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Sample records for microscope phase plate

  1. Ponderomotive phase plate for transmission electron microscopes

    DOEpatents

    Reed, Bryan W [Livermore, CA

    2012-07-10

    A ponderomotive phase plate system and method for controllably producing highly tunable phase contrast transfer functions in a transmission electron microscope (TEM) for high resolution and biological phase contrast imaging. The system and method includes a laser source and a beam transport system to produce a focused laser crossover as a phase plate, so that a ponderomotive potential of the focused laser crossover produces a scattering-angle-dependent phase shift in the electrons of the post-sample electron beam corresponding to a desired phase contrast transfer function.

  2. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

    SciTech Connect

    Iketaki, Yoshinori; Kitagawa, Katsuichi; Hidaka, Kohjiro; Kato, Naoki; Hirabayashi, Akira; Bokor, Nandor

    2014-07-15

    In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

  3. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope.

    PubMed

    Iketaki, Yoshinori; Kitagawa, Katsuichi; Hidaka, Kohjiro; Kato, Naoki; Hirabayashi, Akira; Bokor, Nandor

    2014-07-01

    In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

  4. Design of an electron microscope phase plate using a focused continuous-wave laser

    PubMed Central

    Müller, H; Jin, Jian; Danev, R; Spence, J; Padmore, H; Glaeser, R M

    2010-01-01

    We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser–electron interaction, study resonant cavities for enhancing the laser intensity and discuss applications in biology, soft-materials science and atomic and molecular physics. PMID:20808709

  5. Design of an electron microscope phase plate using a focused continuous-wave laser

    SciTech Connect

    Spence, J.; Muller, H; Jin, Jian; Danev, R; Padmore, H; Glaeser, R.M

    2010-07-01

    We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser–electron interaction, study resonant cavities for enhancing the laser intensity and discuss applications in biology, soft-materials science and atomic and molecular physics.

  6. Electron microscope phase enhancement

    DOEpatents

    Jin, Jian; Glaeser, Robert M.

    2010-06-15

    A microfabricated electron phase shift element is used for modifying the phase characteristics of an electron beam passing though its center aperture, while not affecting the more divergent portion of an incident beam to selectively provide a ninety-degree phase shift to the unscattered beam in the back focal plan of the objective lens, in order to realize Zernike-type, in-focus phase contrast in an electron microscope. One application of the element is to increase the contrast of an electron microscope for viewing weakly scattering samples while in focus. Typical weakly scattering samples include biological samples such as macromolecules, or perhaps cells. Preliminary experimental images demonstrate that these devices do apply a ninety degree phase shift as expected. Electrostatic calculations have been used to determine that fringing fields in the region of the scattered electron beams will cause a negligible phase shift as long as the ratio of electrode length to the transverse feature-size aperture is about 5:1. Calculations are underway to determine the feasibility of aspect smaller aspect ratios of about 3:1 and about 2:1.

  7. Theoretical model of the helium zone plate microscope

    NASA Astrophysics Data System (ADS)

    Salvador Palau, Adrià; Bracco, Gianangelo; Holst, Bodil

    2017-01-01

    Neutral helium microscopy is a new technique currently under development. Its advantages are the low energy, charge neutrality, and inertness of the helium atoms, a potential large depth of field, and the fact that at thermal energies the helium atoms do not penetrate into any solid material. This opens the possibility, among others, for the creation of an instrument that can measure surface topology on the nanoscale, even on surfaces with high aspect ratios. One of the most promising designs for helium microscopy is the zone plate microscope. It consists of a supersonic expansion helium beam collimated by an aperture (skimmer) focused by a Fresnel zone plate onto a sample. The resolution is determined by the focal spot size, which depends on the size of the skimmer, the optics of the system, and the velocity spread of the beam through the chromatic aberrations of the zone plate. An important factor for the optics of the zone plate is the width of the outermost zone, corresponding to the smallest opening in the zone plate. The width of the outermost zone is fabrication limited to around 10 nm with present-day state-of-the-art technology. Due to the high ionization potential of neutral helium atoms, it is difficult to build efficient helium detectors. Therefore, it is crucial to optimize the microscope design to maximize the intensity for a given resolution and width of the outermost zone. Here we present an optimization model for the helium zone plate microscope. Assuming constant resolution and width of the outermost zone, we are able to reduce the problem to a two-variable problem (zone plate radius and object distance) and we show that for a given beam temperature and pressure, there is always a single intensity maximum. We compare our model with the highest-resolution zone plate focusing images published and show that the intensity can be increased seven times. Reducing the width of the outermost zone to 10 nm leads to an increase in intensity of more than 8000

  8. Microscopic Description of Nuclear Quantum Phase Transitions

    SciTech Connect

    Niksic, T.; Vretenar, D.; Lalazissis, G. A.; Ring, P.

    2007-08-31

    The relativistic mean-field framework, extended to include correlations related to restoration of broken symmetries and to fluctuations of the quadrupole deformation, is applied to a study of shape transitions in Nd isotopes. It is demonstrated that the microscopic self-consistent approach, based on global effective interactions, can describe not only general features of transitions between spherical and deformed nuclei, but also the singular properties of excitation spectra and transition rates at the critical point of quantum shape phase transition.

  9. Quantitative phase-contrast confocal microscope

    PubMed Central

    Liu, Changgeng; Marchesini, Stefano; Kim, Myung K.

    2014-01-01

    We present a quantitative phase-contrast confocal microscope (QPCCM) by combining a line-scanning confocal system with digital holography (DH). This combination can merge the merits of these two different imaging modalities. High-contrast intensity images with low coherent noise, and the optical sectioning capability are made available due to the confocality. Phase profiles of the samples become accessible thanks to DH. QPCCM is able to quantitatively measure the phase variations of optical sections of the opaque samples and has the potential to take high-quality intensity and phase images of non-opaque samples such as many biological samples. Because each line scan is recorded by a hologram that may contain the optical aberrations of the system, it opens avenues for a variety of numerical aberration compensation methods and development of full digital adaptive optics confocal system to emulate current hardware-based adaptive optics system for biomedical imaging, especially ophthalmic imaging. Preliminary experiments with a microscope objective of NA 0.65 and 40 × on opaque samples are presented to demonstrate this idea. The measured lateral and axial resolutions of the intensity images from the current system are ~0.64μm and ~2.70μm respectively. The noise level of the phase profile by QPCCM is ~2.4nm which is better than the result by DH. PMID:25089404

  10. Scanning Photoelectron Microscope (SPEM) with a zone plate generated microprobe

    SciTech Connect

    Ade, H.; Kirz, J.; Hulbert, S.; Johnson, E.; Anderson, E.; Kern, D. . Dept. of Physics; Brookhaven National Lab., Upton, NY; Lawrence Berkeley Lab., CA . Center for X-Ray Optics; International Business Machines Corp., Yorktown Heights, NY . Thomas J. Watson Research Center)

    1989-01-01

    We describe instrumentation of a scanning photoelectron microscope (SPEM), which we are presently developing and commissioning at the X1A beamline of the National Synchrotron Light Source (NSLS). This instrument is designed to use the Soft X-ray Undulator (SXU) at the NSLS as a high brightness source to illuminate a Fresnel zone plate, thus forming a finely focused probe, {le} 0.2{mu}m in size, on the specimen surface. A grating monochromator selects the photon energy in the 400-800 eV range with an energy resolution better than 1 eV. The expected flux in the focus is in the 5 {times} 10{sup 7} {minus} 10{sup 9} photons/s range. A single pass Cylindrical Mirror Analyzer (CMA) is used to record photoemission spectra, or to form an image within a fixed electron energy bandwidth as the specimen is mechanically scanned. As a first test, a 1000 mesh Au grid was successfully imaged with Au 4 f primary photoelectrons, achieving a resolution of about 1{mu}m. 10 refs., 5 figs., 1 tab.

  11. Active limited-angle tomographic phase microscope.

    PubMed

    Kus, Arkadiusz; Krauze, Wojciech; Kujawinska, Malgorzata

    2015-01-01

    We demonstrate an active, holographic tomography system, working with limited angle of projections, realized by optical-only, diffraction-based beam steering. The system created for this purpose is a Mach–Zehnder interferometer modified to serve as a digital holographic microscope with a high numerical aperture illumination module and a spatial light modulator (SLM). Such a solution is fast and robust. Apart from providing an elegant solution to viewing angle shifting, it also adds new capabilities of the holographic microscope system. SLM, being an active optical element, allows wavefront correction in order to improve measurement accuracy. Integrated phase data captured with different illumination scenarios within a highly limited angular range are processed by a new tomographic reconstruction algorithm based on the compressed sensing technique: total variation minimization, which is applied here to reconstruct nonpiecewise constant samples. Finally, the accuracy of full measurement and the proposed processing path is tested for a calibrated three-dimensional micro-object as well as a biological object--C2C12 myoblast cell.

  12. Active limited-angle tomographic phase microscope

    NASA Astrophysics Data System (ADS)

    Kuś, Arkadiusz; Krauze, Wojciech; Kujawińska, Małgorzata

    2015-11-01

    We demonstrate an active, holographic tomography system, working with limited angle of projections, realized by optical-only, diffraction-based beam steering. The system created for this purpose is a Mach-Zehnder interferometer modified to serve as a digital holographic microscope with a high numerical aperture illumination module and a spatial light modulator (SLM). Such a solution is fast and robust. Apart from providing an elegant solution to viewing angle shifting, it also adds new capabilities of the holographic microscope system. SLM, being an active optical element, allows wavefront correction in order to improve measurement accuracy. Integrated phase data captured with different illumination scenarios within a highly limited angular range are processed by a new tomographic reconstruction algorithm based on the compressed sensing technique: total variation minimization, which is applied here to reconstruct nonpiecewise constant samples. Finally, the accuracy of full measurement and the proposed processing path is tested for a calibrated three-dimensional micro-object as well as a biological object-C2C12 myoblast cell.

  13. Variable Phase for Fresnel Zone Plates

    NASA Astrophysics Data System (ADS)

    Webb, George W.

    2004-03-01

    It is not widely known that there is a free parameter in the usual design of zone plates. An earlier work treated the radius of the central Fresnel zone as the free parameter and investigated the effects of its variation numerically [1]. It is possible instead to treat the choice of reference phase in the design of a zone plate as the free parameter [2]. The standard zone plate construction assumes a specific choice for this phase which, however, can be chosen to have any value between 0^o and 360^o. Here we present analysis and measurements on zone plates for 39 GHz radiation with reference phase varied from 0^o to 360^o. When the reference phase is varied, measurements show that the phase of the focused beam is varied in a nearly linear fashion through 360^o with only small changes in beam amplitude. It is concluded that reference phase is an inherent and useful property of zone plates. 1) I.V. Minin and O.V. Minin, Sov. J. Quantum Electron. derline 20, 198 (1990). I thank I.V. Minin for calling this work to my attention. 2) G.W. Webb, Proc. 2003 Antenna Applications Symposium, Allerton Park, Monticello, IL, September 15-17, 2003 and arXiv:physics/0303002 28 Feb 2003.

  14. Phase imaging results of phase defect using micro-coherent extreme ultraviolet scatterometry microscope

    NASA Astrophysics Data System (ADS)

    Harada, Tetsuo; Hashimoto, Hiraku; Amano, Tsuyoshi; Kinoshita, Hiroo; Watanabe, Takeo

    2016-04-01

    To evaluate defects on extreme ultraviolet (EUV) masks at the blank state of manufacturing, we developed a micro-coherent EUV scatterometry microscope (micro-CSM). The illumination source is coherent EUV light with a 140 nm focus diameter on the defect using a Fresnel zone plate. This system directly observes the reflection and diffraction signals from a phase defect. The phase and the intensity image of the defect are reconstructed with the diffraction images using ptychography, which is an algorithm of the coherent diffraction imaging. We observed programmed phase defect on a blank EUV mask. Phase distributions of these programmed defects were well reconstructed quantitatively. The micro-CSM is a very powerful tool to review an EUV phase defect.

  15. Observation of a Soft Tissue by a Zernike Phase Contrast Hard X-ray Microscope

    NASA Astrophysics Data System (ADS)

    Aoki, Sadao; Namikawa, Tadahiro; Hoshino, Masato; Watanabe, Norio

    2007-01-01

    A Zernike-type phase contrast hard X-ray microscope was constructed at the Photon Factory BL3C2 (KEK). A white beam from a bending magnet was monochromatized by a silicon double crystal monochromator. Monochromatic parallel X-ray beam illuminated a sample, and transmitted and diffracted X-ray beams were imaged by a Fresnel zone plate (FZP) which had the outer zone width of 100 nm. A phase plate made of a thin aluminum foil with a pinhole was set at the back focal plane of the FZP. The phase plate modulated the diffraction beam from the FZP, whereas a direct beam passed through the pinhole. The resolution of the microscope was measured by observing a tantalum test pattern at an X-ray energy of 9 keV. A 100nm line-and-space pattern could be resolved. X-ray montage pictures of growing eggs of artemia (plankton) were obtained.

  16. Observation of a Soft Tissue by a Zernike Phase Contrast Hard X-ray Microscope

    SciTech Connect

    Aoki, Sadao; Namikawa, Tadahiro; Hoshino, Masato; Watanabe, Norio

    2007-01-19

    A Zernike-type phase contrast hard X-ray microscope was constructed at the Photon Factory BL3C2 (KEK). A white beam from a bending magnet was monochromatized by a silicon double crystal monochromator. Monochromatic parallel X-ray beam illuminated a sample, and transmitted and diffracted X-ray beams were imaged by a Fresnel zone plate (FZP) which had the outer zone width of 100 nm. A phase plate made of a thin aluminum foil with a pinhole was set at the back focal plane of the FZP. The phase plate modulated the diffraction beam from the FZP, whereas a direct beam passed through the pinhole. The resolution of the microscope was measured by observing a tantalum test pattern at an X-ray energy of 9 keV. A 100nm line-and-space pattern could be resolved. X-ray montage pictures of growing eggs of artemia (plankton) were obtained.

  17. Volta potential phase plate for in-focus phase contrast transmission electron microscopy

    PubMed Central

    Danev, Radostin; Buijsse, Bart; Khoshouei, Maryam; Plitzko, Jürgen M.; Baumeister, Wolfgang

    2014-01-01

    We describe a phase plate for transmission electron microscopy taking advantage of a hitherto-unknown phenomenon, namely a beam-induced Volta potential on the surface of a continuous thin film. The Volta potential is negative, indicating that it is not caused by beam-induced electrostatic charging. The film must be heated to ∼200 °C to prevent contamination and enable the Volta potential effect. The phase shift is created “on the fly” by the central diffraction beam eliminating the need for precise phase plate alignment. Images acquired with the Volta phase plate (VPP) show higher contrast and unlike Zernike phase plate images no fringing artifacts. Following installation into the microscope, the VPP has an initial settling time of about a week after which the phase shift behavior becomes stable. The VPP has a long service life and has been used for more than 6 mo without noticeable degradation in performance. The mechanism underlying the VPP is the same as the one responsible for the degradation over time of the performance of thin-film Zernike phase plates, but in the VPP it is used in a constructive way. The exact physics and/or chemistry behind the process causing the Volta potential are not fully understood, but experimental evidence suggests that radiation-induced surface modification combined with a chemical equilibrium between the surface and residual gases in the vacuum play an important role. PMID:25331897

  18. Phase-Scrambler Plate Spreads Point Image

    NASA Technical Reports Server (NTRS)

    Edwards, Oliver J.; Arild, Tor

    1992-01-01

    Array of small prisms retrofit to imaging lens. Phase-scrambler plate essentially planar array of small prisms partitioning aperture of lens into many subapertures, and prism at each subaperture designed to divert relatively large diffraction spot formed by that subaperture to different, specific point on focal plane.

  19. Carbon contamination in scanning transmission electron microscopy and its impact on phase-plate applications.

    PubMed

    Hettler, Simon; Dries, Manuel; Hermann, Peter; Obermair, Martin; Gerthsen, Dagmar; Malac, Marek

    2017-05-01

    We analyze electron-beam induced carbon contamination in a transmission electron microscope. The study is performed on thin films potentially suitable as phase plates for phase-contrast transmission electron microscopy. Electron energy-loss spectroscopy and phase-plate imaging is utilized to analyze the contamination. The deposited contamination layer is identified as a graphitic carbon layer which is not prone to electrostatic charging whereas a non-conductive underlying substrate charges. Several methods that inhibit contamination are evaluated and the impact of carbon contamination on phase-plate imaging is discussed. The findings are in general interesting for scanning transmission electron microscopy applications.

  20. In-focus electron microscopy of frozen-hydrated biological samples with a Boersch phase plate.

    PubMed

    Barton, B; Rhinow, D; Walter, A; Schröder, R; Benner, G; Majorovits, E; Matijevic, M; Niebel, H; Müller, H; Haider, M; Lacher, M; Schmitz, S; Holik, P; Kühlbrandt, W

    2011-12-01

    We report the implementation of an electrostatic Einzel lens (Boersch) phase plate in a prototype transmission electron microscope dedicated to aberration-corrected cryo-EM. The combination of phase plate, C(s) corrector and Diffraction Magnification Unit (DMU) as a new electron-optical element ensures minimal information loss due to obstruction by the phase plate and enables in-focus phase contrast imaging of large macromolecular assemblies. As no defocussing is necessary and the spherical aberration is corrected, maximal, non-oscillating phase contrast transfer can be achieved up to the information limit of the instrument. A microchip produced by a scalable micro-fabrication process has 10 phase plates, which are positioned in a conjugate, magnified diffraction plane generated by the DMU. Phase plates remained fully functional for weeks or months. The large distance between phase plate and the cryo sample permits the use of an effective anti-contaminator, resulting in ice contamination rates of <0.6 nm/h at the specimen. Maximal in-focus phase contrast was obtained by applying voltages between 80 and 700 mV to the phase plate electrode. The phase plate allows for in-focus imaging of biological objects with a signal-to-noise of 5-10 at a resolution of 2-3 nm, as demonstrated for frozen-hydrated virus particles and purple membrane at liquid-nitrogen temperature.

  1. Interference Confocal Microscope Integrated with Spatial Phase Shifter

    PubMed Central

    Wang, Weibo; Gu, Kang; You, Xiaoyu; Tan, Jiubin; Liu, Jian

    2016-01-01

    We present an interference confocal microscope (ICM) with a new single-body four-step simultaneous phase-shifter device designed to obtain high immunity to vibration. The proposed ICM combines the respective advantages of simultaneous phase shifting interferometry and bipolar differential confocal microscopy to obtain high axis resolution, large dynamic range, and reduce the sensitivity to vibration and reflectance disturbance seamlessly. A compact single body spatial phase shifter is added to capture four phase-shifted interference signals simultaneously without time delay and construct a stable and space-saving simplified interference confocal microscope system. The test result can be obtained by combining the interference phase response and the bipolar property of differential confocal microscopy without phase unwrapping. Experiments prove that the proposed microscope is capable of providing stable measurements with 1 nm of axial depth resolution for either low- or high-numerical aperture objective lenses. PMID:27563909

  2. Interference Confocal Microscope Integrated with Spatial Phase Shifter.

    PubMed

    Wang, Weibo; Gu, Kang; You, Xiaoyu; Tan, Jiubin; Liu, Jian

    2016-08-24

    We present an interference confocal microscope (ICM) with a new single-body four-step simultaneous phase-shifter device designed to obtain high immunity to vibration. The proposed ICM combines the respective advantages of simultaneous phase shifting interferometry and bipolar differential confocal microscopy to obtain high axis resolution, large dynamic range, and reduce the sensitivity to vibration and reflectance disturbance seamlessly. A compact single body spatial phase shifter is added to capture four phase-shifted interference signals simultaneously without time delay and construct a stable and space-saving simplified interference confocal microscope system. The test result can be obtained by combining the interference phase response and the bipolar property of differential confocal microscopy without phase unwrapping. Experiments prove that the proposed microscope is capable of providing stable measurements with 1 nm of axial depth resolution for either low- or high-numerical aperture objective lenses.

  3. Observation of Phase Objects by Using an X-ray Microscope with a Foucault Knife-Edge

    SciTech Connect

    Watanabe, N.; Sasaya, T.; Imai, Y.; Iwata, S.; Zama, K.; Aoki, S.

    2011-09-09

    An x-ray microscope with a zone plate was assembled at the synchrotron radiation source of BL3C, Photon Factory. A Foucault knife-edge was set at the back focal plate of the objective zone plate and phase retrieval was tested by scanning the knife-edge. A preliminary result shows that scanning the knife-edge during exposure was effective for phase retrieval. Phase-contrast tomography was investigated using differential projection images calculated from two Schlieren images with the oppositely oriented knife-edges. Fairly good reconstruction images of polystyrene beads and spores could be obtained.

  4. Observation of Phase Objects by Using an X-ray Microscope with a Foucault Knife-Edge

    NASA Astrophysics Data System (ADS)

    Watanabe, N.; Sasaya, T.; Imai, Y.; Iwata, S.; Zama, K.; Aoki, S.

    2011-09-01

    An x-ray microscope with a zone plate was assembled at the synchrotron radiation source of BL3C, Photon Factory. A Foucault knife-edge was set at the back focal plate of the objective zone plate and phase retrieval was tested by scanning the knife-edge. A preliminary result shows that scanning the knife-edge during exposure was effective for phase retrieval. Phase-contrast tomography was investigated using differential projection images calculated from two Schlieren images with the oppositely oriented knife-edges. Fairly good reconstruction images of polystyrene beads and spores could be obtained.

  5. Intracellular dynamics with the phase microscope Airyscan

    NASA Astrophysics Data System (ADS)

    Tychinsky, Vladimir P.; Perevedentseva, Elena V.; Vyshenskaia, Tatiana V.; Kufal, Georgy E.

    1997-12-01

    Investigation of intracellular dynamics of Allium cepa inner epidermal cells are described. The applicability of the method for quantitative estimation of spatio-temporal phase fluctuations and the effect due to external factors is discussed. The analysis of time-sampled series allows one to detect the regions of various motility in cytoplasm. The intense Fourier-spectra harmonics in 0.2 - 8 Hz interval were observed inside a cell wall and cytoplasm. Regularly spaced 2- to 4-s long batches of 100-ms pulses at cell-wall sites are recorded. The phase-fluctuation intensity decreased and the frequencies of certain harmonics were shifted with lowering temperature. The advantages and specific features of the method are discussed.

  6. Smartphone based hand-held quantitative phase microscope using the transport of intensity equation method.

    PubMed

    Meng, Xin; Huang, Huachuan; Yan, Keding; Tian, Xiaolin; Yu, Wei; Cui, Haoyang; Kong, Yan; Xue, Liang; Liu, Cheng; Wang, Shouyu

    2016-12-20

    In order to realize high contrast imaging with portable devices for potential mobile healthcare, we demonstrate a hand-held smartphone based quantitative phase microscope using the transport of intensity equation method. With a cost-effective illumination source and compact microscope system, multi-focal images of samples can be captured by the smartphone's camera via manual focusing. Phase retrieval is performed using a self-developed Android application, which calculates sample phases from multi-plane intensities via solving the Poisson equation. We test the portable microscope using a random phase plate with known phases, and to further demonstrate its performance, a red blood cell smear, a Pap smear and monocot root and broad bean epidermis sections are also successfully imaged. Considering its advantages as an accurate, high-contrast, cost-effective and field-portable device, the smartphone based hand-held quantitative phase microscope is a promising tool which can be adopted in the future in remote healthcare and medical diagnosis.

  7. Acoustic microscope based on magneto-elastic wave phase conjugator

    NASA Astrophysics Data System (ADS)

    Brysev, A.; Krutyansky, L.; Pernod, P.; Preobrazhensky, V.

    2000-05-01

    Acoustic C-scan imaging (acoustic microscopy) by means of supercritical parametric wave phase conjugation (WPC) is studied experimentally. A phase conjugator based on a magneto-acoustic active material is used for compensating phase distortions introduced by solid and polymer aberration layers covering objects (electronic integrated circuits as examples). Improvement of images is demonstrated on an acoustic microscope, operating at a frequency of 10 MHz.

  8. Differential phase acoustic microscope for micro-NDE

    NASA Technical Reports Server (NTRS)

    Waters, David D.; Pusateri, T. L.; Huang, S. R.

    1992-01-01

    A differential phase scanning acoustic microscope (DP-SAM) was developed, fabricated, and tested in this project. This includes the acoustic lens and transducers, driving and receiving electronics, scanning stage, scanning software, and display software. This DP-SAM can produce mechanically raster-scanned acoustic microscopic images of differential phase, differential amplitude, or amplitude of the time gated returned echoes of the samples. The differential phase and differential amplitude images provide better image contrast over the conventional amplitude images. A specially designed miniature dual beam lens was used to form two foci to obtain the differential phase and amplitude information of the echoes. High image resolution (1 micron) was achieved by applying high frequency (around 1 GHz) acoustic signals to the samples and placing two foci close to each other (1 micron). Tone burst was used in this system to obtain a good estimation of the phase differences between echoes from the two adjacent foci. The system can also be used to extract the V(z) acoustic signature. Since two acoustic beams and four receiving modes are available, there are 12 possible combinations to produce an image or a V(z) scan. This provides a unique feature of this system that none of the existing acoustic microscopic systems can provide for the micro-nondestructive evaluation applications. The entire system, including the lens, electronics, and scanning control software, has made a competitive industrial product for nondestructive material inspection and evaluation and has attracted interest from existing acoustic microscope manufacturers.

  9. Comparative study viruses with computer-aided phase microscope AIRYSCAN

    NASA Astrophysics Data System (ADS)

    Tychinsky, Vladimir P.; Koufal, Georgy E.; Perevedentseva, Elena V.; Vyshenskaia, Tatiana V.

    1996-12-01

    Traditionally viruses are studied with scanning electron microscopy (SEM) after complicated procedure of sample preparation without the possibility to study it under natural conditions. We obtained images of viruses (Vaccinia virus, Rotavirus) and rickettsias (Rickettsia provazekii, Coxiella burnetti) in native state with computer-aided phase microscope airyscan -- the interference microscope of Linnik layout with phase modulation of the reference wave with dissector image tube as coordinate-sensitive photodetector and computer processing of phase image. A light source was the He-Ne laser. The main result is coincidence of dimensions and shape of phase images with available information concerning their morphology obtained with SEM and other methods. The fine structure of surface and nuclei is observed. This method may be applied for virus recognition and express identification, investigation of virus structure and the analysis of cell-virus interaction.

  10. Plate-to-plate fluorous solid-phase extraction for solution-phase parallel synthesis.

    PubMed

    Zhang, Wei; Lu, Yimin; Nagashima, Tadamichi

    2005-01-01

    A commercially available Argonaut VacMaster-96 plate-to-plate solid-phase extraction (SPE) station equipped with 24 FluoroFlash cartridges is employed for parallel purification of fluorous reaction mixtures. Each cartridge charged with 3 g of fluorous silica gel has the capability to produce up to 100 mg of purified small molecules. The 24-well receiving plate has a standard footprint that can be directly concentrated in a Genevac vacuum centrifuge. Important issues such as sample loading, product cross-contamination, cartridge reuse, and reproducibility are investigated. The SPE system has been demonstrated in the purification of three small libraries that were produced involving amine scavenging reactions with fluorous isatoic anhydride, amide coupling reactions with 2-chloro-4,6-bis[(perfluorohexyl)propyloxy]-1,3,5-triazine (fluorous CDMT), and amide coupling reactions with a newly developed fluorous Mukaiyama condensation reagent.

  11. Observation results of actual phase defects using micro coherent EUV scatterometry microscope

    NASA Astrophysics Data System (ADS)

    Hashimoto, Hiraku; Harada, Tetsuo; Watanabe, Takeo

    2016-10-01

    One of the critical issue of EUV lithography is fabrication of defect-free mask. The origin of the defect is a particle inside the multilayer and bump or pit on glass substrate. This type of defect is called a phase defect. If there is a phase defect, the reflection phase is disordered. As a result, the phase structure is printed as a defect on a wafer. Thus, we have developed micro coherent EUV scatterometry microscope (we called micro-CSM) for phase defect characterization. Micro-CSM records scattering signal from a defect directly exposed by focused coherent EUV having a spot size of φ140-nm in diameter. An off-axis-type Fresnel zone plate was employed as a focusing optics. Phase distribution of the defect is reconstructed with the scattering image by the coherent-diffraction-imaging method. We observed actual phase defects in this work. Actual phase defects were on a mask blanks which was the same grade of the pre-production mask of the semiconductor devices. The positions of actual phase defects have been already inspected by the actinic blank inspection tool. And, the actual phase defects have been already observed using an atomic force microscope. A purpose of this work is observation of these actual defects using micro-CSM and comparison of the results.

  12. Quantitative phase imaging by wide field lensless digital holographic microscope

    NASA Astrophysics Data System (ADS)

    Adinda-Ougba, A.; Koukourakis, N.; Essaidi, A.; Ger­hardt, N. C.; Hofmann, M. R.

    2015-05-01

    Wide field, lensless microscopes have been developed for telemedicine and for resource limited setting [1]. They are based on in-line digital holography which is capable to provide amplitude and phase information resulting from numerical reconstruction. The phase information enables achieving axial resolution in the nanometer range. Hence, such microscopes provide a powerful tool to determine three-dimensional topologies of microstructures. In this contribution, a compact, low-cost, wide field, lensless microscope is presented, which is capable of providing topological profiles of microstructures in transparent material. Our setup consist only of two main components: a CMOSsensor chip and a laser diode without any need of a pinhole. We use this very simple setup to record holograms of microobjects. A wide field of view of ~24 mm², and a lateral resolution of ~2 μm are achieved. Moreover, amplitude and phase information are obtained from the numerical reconstruction of the holograms using a phase retrieval algorithm together with the angular spectrum propagation method. Topographic information of highly transparent micro-objects is obtained from the phase data. We evaluate our system by recording holograms of lines with different depths written by a focused laser beam. A reliable characterization of laser written microstructures is crucial for their functionality. Our results show that this system is valuable for determination of topological profiles of microstructures in transparent material.

  13. Microscopic imaging of residual stress using a scanning phase-measuring acoustic microscope

    NASA Astrophysics Data System (ADS)

    Meeks, Steven W.; Peter, D.; Horne, D.; Young, K.; Novotny, V.

    1989-10-01

    A high-resolution scanning phase-measuring acoustic microscope (SPAM) has been developed and used to image the near-surface residual stress field around features etched in sputtered alumina via the acoustoelastic effect. This microscope operates at 670 MHz and has a resolution of 5-10 microns, depending upon the amount of defocus. Relative velocity changes of sample surface waves as small as 50 ppm are resolved. Images of the stress field at the tip of a 400-micron-wide slot etched in alumina are presented and compared with a finite element simulation. The SPAM uses an unconventional acoustic lens with an anisotropic illumination pattern which can measure anisotropic effects and map residual stress fields with several-micron resolution and a stress sensitivity of 1/3 MPa in an alumina film.

  14. Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope

    PubMed Central

    Han, Chao; Lee, Lap Man; Yang, Changhuei

    2013-01-01

    We report the implementation of an on-chip microscope system, termed fluorescence optofluidic microscope (FOFM), which is capable of fluorescence microscopy imaging of samples in fluid media. The FOFM employs an array of Fresnel zone plates (FZP) to generate an array of focused light spots within a microfluidic channel. As a sample flows through the channel and across the array of focused light spots, the fluorescence emissions are collected by a filter-coated CMOS sensor, which serves as the channel's floor. The collected data can then be processed to render fluorescence microscopy images at a resolution determined by the focused light spot size (experimentally measured as 0.65 μm FWHM). In our experiments, our established resolution was 1.0 μm due to Nyquist criterion consideration. As a demonstration, we show that such a system can be used to image the cell nuclei stained by Acridine Orange and cytoplasm labeled by Qtracker®. PMID:21935556

  15. Phases and phase transitions in the algebraic microscopic shell model

    NASA Astrophysics Data System (ADS)

    Georgieva, A. I.; Drumev, K. P.

    2016-01-01

    We explore the dynamical symmetries of the shell model number conserving algebra, which define three types of pairing and quadrupole phases, with the aim to obtain the prevailing phase or phase transition for the real nuclear systems in a single shell. This is achieved by establishing a correspondence between each of the pairing bases with the Elliott's SU(3) basis that describes collective rotation of nuclear systems. This allows for a complete classification of the basis states of different number of particles in all the limiting cases. The probability distribution of the SU(3) basis states within theirs corresponding pairing states is also obtained. The relative strengths of dynamically symmetric quadrupole-quadrupole interaction in respect to the isoscalar, isovector and total pairing interactions define a control parameter, which estimates the importance of each term of the Hamiltonian in the correct reproduction of the experimental data for the considered nuclei.

  16. Microscopic analysis of order parameters in nuclear quantum phase transitions

    SciTech Connect

    Li, Z. P.; Niksic, T.; Vretenar, D.; Meng, J.

    2009-12-15

    Microscopic signatures of nuclear ground-state shape phase transitions in Nd isotopes are studied using excitation spectra and collective wave functions obtained by diagonalization of a five-dimensional Hamiltonian for quadrupole vibrational and rotational degrees of freedom, with parameters determined by constrained self-consistent relativistic mean-field calculations for triaxial shapes. As a function of the physical control parameter, the number of nucleons, energy gaps between the ground state and the excited vibrational states with zero angular momentum, isomer shifts, and monopole transition strengths exhibit sharp discontinuities at neutron number N=90, which is characteristic of a first-order quantum phase transition.

  17. The North American Astronomical Photographic Plate Center: Phase I.

    NASA Astrophysics Data System (ADS)

    Cline, J. D.; Castelaz, M. W.; Crowley, T.; Griffin, E.; Osborn, W.

    2004-05-01

    Astronomical photographic plates constitute an important and, for the large part, unrepeatable resource for research. International pressure is mounting to preserve and catalog scientifically valuable plate collections and capture their information through digitization. At the same time, many institutions holding plates now lack the space, funds and expertise to adequately preserve this important material. In response, the Pisgah Astronomical Research Institute has established the North American Photographic Plate Center (NAPPC). NAPPC is intended as a long-term repository for direct and objective prism plate collections currently stored in North America. PARI is a natural location for such a center. It offers physically secure and abundant environmentally controlled space for plate storage as well as Internet 2 infrastructure and instrument space necessary for the eventual digitization and Internet distribution of images. Phase I of this initiative is to collect unwanted plate collections, store them in an appropriate manner, prepare catalogues of their relevant information and establish a laboratory for on-site examination or measurement of the plates. This is currently underway. Phase II will be the eventual digitization and development of a public web accessible database of images. We will describe the procedures for placing plate collections in NAPPC, the infrastructure in place for plate storage and measurement, and our preliminary plans for making the plate archive a public image library with Internet access.

  18. High precision refractometry based on Fresnel diffraction from phase plates.

    PubMed

    Tavassoly, M Taghi; Naraghi, Roxana Rezvani; Nahal, Arashmid; Hassani, Khosrow

    2012-05-01

    When a transparent plane-parallel plate is illuminated at a boundary region by a monochromatic parallel beam of light, Fresnel diffraction occurs because of the abrupt change in phase imposed by the finite change in refractive index at the plate boundary. The visibility of the diffraction fringes varies periodically with changes in incident angle. The visibility period depends on the plate thickness and the refractive indices of the plate and the surrounding medium. Plotting the phase change versus incident angle or counting the visibility repetition in an incident-angle interval provides, for a given plate thickness, the refractive index of the plate very accurately. It is shown here that the refractive index of a plate can be determined without knowing the plate thickness. Therefore, the technique can be utilized for measuring plate thickness with high precision. In addition, by installing a plate with known refractive index in a rectangular cell filled with a liquid and following the described procedures, the refractive index of the liquid is obtained. The technique is applied to measure the refractive indices of a glass slide, distilled water, and ethanol. The potential and merits of the technique are also discussed.

  19. Testing and Calibration of Phase Plates for JWST Optical Simulator

    NASA Technical Reports Server (NTRS)

    Gong, Qian; Chu, Jenny; Tournois, Severine; Eichhorn, William; Kubalak, David

    2011-01-01

    Three phase plates were designed to simulate the JWST segmented primary mirror wavefront at three on-orbit alignment stages: coarse phasing, intermediate phasing, and fine phasing. The purpose is to verify JWST's on-orbit wavefront sensing capability. Amongst the three stages, coarse alignment is defined to have piston error between adjacent segments being 30 m to 300 m, intermediate being 0.4 m to 10 m, and fine is below 0.4 m. The phase plates were made of fused silica, and were assembled in JWST Optical Simulator (OSIM). The piston difference was realized by the thickness difference of two adjacent segments. The two important parameters to phase plates are piston and wavefront errors. Dispersed Fringe Sensor (DFS) method was used for initial coarse piston evaluation, which is the emphasis of this paper. Point Diffraction Interferometer (PDI) is used for fine piston and wavefront error. In order to remove piston's 2 pi uncertainty with PDI, three laser wavelengths, 640nm, 660nm, and 780nm, are used for the measurement. The DHS test setup, analysis algorithm and results are presented. The phase plate design concept and its application (i.e. verifying the JWST on-orbit alignment algorithm) are described. The layout of JWST OSIM and the function of phase plates in OSIM are also addressed briefly.

  20. Multi-pore carbon phase plate for phase-contrast transmission electron microscopy.

    PubMed

    Sannomiya, Takumi; Junesch, Juliane; Hosokawa, Fumio; Nagayama, Kuniaki; Arai, Yoshihiro; Kayama, Yoko

    2014-11-01

    A new fabrication method of carbon based phase plates for phase-contrast transmission electron microscopy is presented. This method utilizes colloidal masks to produce pores as well as disks on thin carbon membranes for phase modulation. Since no serial process is involved, carbon phase plate membranes containing hundreds of pores can be mass-produced on a large scale, which allows "disposal" of contaminated or degraded phase modulating objects after use. Due to the spherical shape of the mask colloid particles, the produced pores are perfectly circular. The pore size and distribution can be easily tuned by the mask colloid size and deposition condition. By using the stencil method, disk type phase plates can also be fabricated on a pore type phase plate. Both pore and disk type phase plates were tested by measuring amorphous samples and confirmed to convert the sinus phase contrast transfer function to the cosine shape.

  1. Dynamic phase imaging utilizing a 4-dimensional microscope system

    PubMed Central

    Creath, Katherine

    2011-01-01

    This paper describes a new, novel interference Linnik microscope system and presents images and data of live biological samples. The specially designed optical system enables instantaneous 4-dimensional video measurements of dynamic motions within and among live cells without the need for contrast agents. This "label-free", vibration insensitive imaging system enables measurement of biological objects in reflection using harmless light levels with a variety of magnifications and wavelengths with fields of view from several hundred microns up to a millimeter. At the core of the instrument is a phase measurement camera (PMC) enabling simultaneous measurement of multiple interference patterns utilizing a pixelated phase mask taking advantage of the polarization properties of light. Utilizing this technology enables the creation of phase image movies in real time at video rates so that dynamic motions and volumetric changes can be tracked. Objects are placed on a reflective surface in liquid under a coverslip. Phase values are converted to optical thickness data enabling volumetric, motion and morphological studies. Data from a number of different organisms such as flagellates and rotifers will be presented, as will measurements of human breast cancer cells with the addition of various agents that break down the cells. These data highlight examples of monitoring different biological processes and motions. PMID:24357901

  2. Understanding oxide interfaces: From microscopic imaging to electronic phases

    NASA Astrophysics Data System (ADS)

    Ilani, Shahal

    2014-03-01

    In the last decade, the advent of complex oxide interfaces has unleashed a wealth of new possibilities to create materials with unexpected functionalities. A notable example is the two-dimensional electron system formed at the interface between LaAlO3 and SrTiO3 (LAO/STO), which exhibits ferromagnetism, superconductivity, and a wide range of unique magneto-transport properties. A key challenge is to find the microscopic mechanisms that underlie these emergent phenomena. While there is a growing understanding that these phenomena might reflect rich structures at the micro-scale, experimental progress toward microscopic imaging of this system has been so far rather limited due to the buried nature of its interface. In this talk I will discuss our experiments that study this system on microscopic and macroscopic scales. Using a newly-developed nanotube-based scanning electrometer we image on the nanoscale the electrostatics and mechanics of this buried interface. We reveal the dynamics of structural domains in STO, their role in generating the contested anomalous piezoelectricity of this substrate, and their direct effects on the physics of the interface electrons. Using macroscopic magneto-transport experiments we demonstrate that a universal Lifshitz transition between the population of d-orbitals with different symmetries underlies many of the transport phenomena observed to date. We further show that the interactions between the itinerant electrons and localized spins leads to an unusual, gate-tunable magnetic phase diagram. These measurements highlight the unique physical settings that can be realized within this new class of low dimensional systems.

  3. Virus structure using the computer-aided phase microscope Airyscan

    NASA Astrophysics Data System (ADS)

    Tychinsky, Vladimir P.; Kaverin, Nikolai V.; Perevedentseva, Elena V.; Vyshenskaia, Tatiana V.; Kufal, Georgy E.

    1997-04-01

    Investigation of features and functions of some small biological objects (smaller than 500 nm), in particular, viruses, with conventional optical microscopy is practically impossible. Usually their images are obtained with methods of scanning electron microscopy (SEM), which precludes work with samples in a native state. We obtained images of different viruses including influenza A virus in native state with computer-aided phase microscope (CPM) Airyscan, in which an He-Ne laser is used as a light source. The main purpose of this work was to show the possibility to obtain adequate structure images of influenza viruses with diameter about 100 nm in conditions quite close to native and to investigate different stages of influenza virus budding. We suppose that these results may be considered as a basis for further studies of cell-virus interaction.

  4. Guided wave phased array beamforming and imaging in composite plates.

    PubMed

    Yu, Lingyu; Tian, Zhenhua

    2016-05-01

    This paper describes phased array beamforming using guided waves in anisotropic composite plates. A generic phased array algorithm is presented, in which direction dependent guided wave parameters and the energy skew effect are considered. This beamforming at an angular direction is achieved based on the classic delay-and-sum principle by applying phase delays to signals received at array elements and adding up the delayed signals. The phase delays are determined with the goal to maximize the array output at the desired direction and minimize it otherwise. For array characterization, the beam pattern of rectangular grid arrays in composite plates is derived. In addition to the beam pattern, the beamforming factor in terms of wavenumber distribution is defined to provide intrinsic explanations for phased array beamforming. The beamforming and damage detection in a composite plate are demonstrated using rectangular grid arrays made by a non-contact scanning laser Doppler vibrometer. Detection images of the composite plate with multiple surface defects at various directions are obtained. The results show that the guided wave phased array method is a potential effective method for rapid inspection of large composite structures.

  5. Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics.

    PubMed

    Marko, Michael; Meng, Xing; Hsieh, Chyongere; Roussie, James; Striemer, Christopher

    2013-11-01

    Imaging with Zernike phase plates is increasingly being used in cryo-TEM tomography and cryo-EM single-particle applications. However, rapid ageing of the phase plates, together with the cost and effort in producing them, present serious obstacles to widespread adoption. We are experimenting with phase plates based on silicon chips that have thin windows; such phase plates could be mass-produced and made available at moderate cost. The windows are coated with conductive layers to reduce charging, and this considerably extends the useful life of the phase plates compared to traditional pure-carbon phase plates. However, a compromise must be reached between robustness and transmission through the phase-plate film. Details are given on testing phase-plate performance by means of imaging an amorphous thin film and evaluating the power spectra of the images.

  6. Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics

    PubMed Central

    Marko, Michael; Meng, Xing; Hsieh, Chyongere; Roussie, James; Striemer, Christopher

    2013-01-01

    Imaging with Zernike phase plates is increasingly being used in cryo-TEM tomography and cryo-EM single-particle applications. However, rapid ageing of the phase plates, together with the cost and effort in producing them, present serious obstacles to widespread adoption. We are experimenting with phase plates based on silicon chips that have thin windows; such phase plates could be mass-produced and made available at moderate cost. The windows are coated with conductive layers to reduce charging, and this considerably extends the useful life of the phase plates compared to traditional pure-carbon phase plates. However, a compromise must be reached between robustness and transmission through the phase-plate film. Details are given on testing phase-plate performance by means of imaging an amorphous thin film and evaluating the power spectra of the images. PMID:23994351

  7. Application of optical diffraction method in designing phase plates

    NASA Astrophysics Data System (ADS)

    Lei, Ze-Min; Sun, Xiao-Yan; Lv, Feng-Nian; Zhang, Zhen; Lu, Xing-Qiang

    2016-11-01

    Continuous phase plate (CPP), which has a function of beam shaping in laser systems, is one kind of important diffractive optics. Based on the Fourier transform of the Gerchberg-Saxton (G-S) algorithm for designing CPP, we proposed an optical diffraction method according to the real system conditions. A thin lens can complete the Fourier transform of the input signal and the inverse propagation of light can be implemented in a program. Using both of the two functions can realize the iteration process to calculate the near-field distribution of light and the far-field repeatedly, which is similar to the G-S algorithm. The results show that using the optical diffraction method can design a CPP for a complicated laser system, and make the CPP have abilities of beam shaping and phase compensation for the phase aberration of the system. The method can improve the adaptation of the phase plate in systems with phase aberrations.

  8. Brewster Angle Microscope Investigations of Two Dimensional Phase Transitions

    NASA Astrophysics Data System (ADS)

    Schuman, Adam William

    The liquid-liquid interface is investigated by microscopic and thermodynamic means to image and measure interfacial properties when the system undergoes a two-dimensional (2D) phase transition of a Gibbs monolayer by varying the sample temperature. An in-house Brewster angle microscope (BAM) is constructed to visualize the interface during this transition while a quasi-elastic light scattering technique is used to determine the interfacial tension. These results complement x-ray investigations of the same systems. Evidence of interfacial micro-separated structure, microphases, comes from observations across a hexane-water interface with the inclusion of a long-chain fluorinated alcohol surfactant into the bulk hexane. Microphases take the form of spatially modulated structure to the density of the surfactant as it spans laterally across the interface. The surfactant monolayer exhibits microphase morphology over a range of a couple degrees as the temperature of the system is scanned through the 2D gas-solid phase transition. Microphase structure was observed for heating and cooling the hexane-water system and structural comparisons are given when the temperature step and quench depth of the cooling process is varied. A complete sequence of morphological structure was observed from 2D gas to cluster to labyrinthine stripe to a 2D solid mosaic pattern. Two characteristic length scales emerge giving rise to speculation of an elastic contribution to the standard repulsive and attractive competitive forces stabilizing the microphase. The benefit of BAM to laterally image very thin films across the surface of an interface on the micrometer length scale nicely complements x-ray reflectivity methods that average structural data transverse to the liquid interface on a molecular scale. To properly analyze x-ray reflectivity data, the interface is required to be laterally homogeneous. BAM can sufficiently characterize the interface for this purpose as is done for a Langmuir

  9. Fraunhofer diffraction of a partially blocked spiral phase plate.

    PubMed

    Cottrell, Don M; Davis, Jeffrey A; Hernandez, Travis J

    2011-07-04

    The Fraunhofer diffraction pattern from a partially blocked spiral phase plate (SPP) produces a partial vortex output pattern that is rotated by 90 degrees compared with the input. The rotation direction depends on whether the angular phase pattern increases in the clockwise or counterclockwise direction. In this work, we present an explanation of this effect based on careful examination of classical diffraction theory and show new experimental results. This approach is very convenient for easily determining the sign of the vortex charge.

  10. Phase plate technology for laser marking of magnetic discs

    DOEpatents

    Neuman, Bill; Honig, John; Hackel, Lloyd; Dane, C. Brent; Dixit, Shamasundar

    1998-01-01

    An advanced design for a phase plate enables the distribution of spots in arbitrarily shaped patterns with very high uniformity and with a continuously or near-continuously varying phase pattern. A continuous phase pattern eliminates large phase jumps typically expected in a grating that provides arbitrary shapes. Large phase jumps increase scattered light outside of the desired pattern, reduce efficiency and can make the grating difficult to manufacture. When manufacturing capabilities preclude producing a fully continuous grating, the present design can be easily adapted to minimize manufacturing errors and maintain high efficiencies. This continuous grating is significantly more efficient than previously described Dammann gratings, offers much more flexibility in generating spot patterns and is easier to manufacture and replicate than a multi-level phase grating.

  11. Phase plate technology for laser marking of magnetic discs

    DOEpatents

    Neuman, B.; Honig, J.; Hackel, L.; Dane, C.B.; Dixit, S.

    1998-10-27

    An advanced design for a phase plate enables the distribution of spots in arbitrarily shaped patterns with very high uniformity and with a continuously or near-continuously varying phase pattern. A continuous phase pattern eliminates large phase jumps typically expected in a grating that provides arbitrary shapes. Large phase jumps increase scattered light outside of the desired pattern, reduce efficiency and can make the grating difficult to manufacture. When manufacturing capabilities preclude producing a fully continuous grating, the present design can be easily adapted to minimize manufacturing errors and maintain high efficiencies. This continuous grating is significantly more efficient than previously described Dammann gratings, offers much more flexibility in generating spot patterns and is easier to manufacture and replicate than a multi-level phase grating. 3 figs.

  12. Kinoform phase plates for focal plane irradiance profile control

    SciTech Connect

    Dixit, S.N.; Lawson, J.K.; Manes, K.R.; Powell, H.T. ); Nugent, K.A. )

    1994-03-15

    A versatile, rapidly convergent, iterative algorithm is presented for the construction of kinoform phase plates for tailoring the far-field intensity distribution of laser beams. The method consists of repeated Fourier transforming between the near-field and the far-field planes with constraints imposed in each plane. For application to inertial confinement fusion, the converged far-field pattern contains more than 95% of the incident energy inside a desired region and is relatively insensitive to beam aberrations.

  13. Improvement of reconstructed phase distribution of fast moving phase object in digital holographic microscope

    NASA Astrophysics Data System (ADS)

    Xia, Peng; Nagahama, Naoya; Quan, Xiangyu; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2016-03-01

    For defect detection or undesired object in commercial products, it is required to develop a fast measurement system that can obtain three-dimensional distribution of surface of the opaque medium such as metal or inside of the transparent medium. For this purpose, we fabricated a digital holographic microscope using a fast image sensor when the phase object is put on a fast movable stage. In the fabricated system, an image sensor operated at maximum frame rate of 2000 fps and a movable stage operated at maximum speed of 300 mm/s are introduced. Under the continuous wave illumination, motion-blurred phase object is reconstructed. By using numerical processing such as deconvolution filter, the reconstructed phase distribution is much improved. Numerical results are presented.

  14. Conversion of tribasic lead sulfate to lead dioxide in lead/acid battery plates. 1: Relationship between the phase compositions of plates in the cured and formed states

    NASA Astrophysics Data System (ADS)

    Zerroual, L.; Chelali, N.; Tedjar, F.; Guitton, J.

    1994-10-01

    The influence of the initial amount of H2SO4 added to lead powder on the phase compositions of plates in the cured and formed states has been studied. IR spectra, x-ray diffraction, scanning electron microscope observations, and wet-chemical analysis are used as techniques of investigation. It was found that the phase composition of the paste depends on the H2SO4:oxidized lead powder ratio. In addition, it is found that alpha- and beta-PbO2 are formed in the lead/acid battery positive plate from 3PbO center-dot PbSO4 center-dot H2O. The amounts of these two compounds were strongly affected when varying the quantity of H2SO4 with respect to the lead powder.

  15. Microstructure and Phase Constitution Near the Interface of Explosively Welded Aluminum/Copper Plates

    NASA Astrophysics Data System (ADS)

    Paul, Henryk; Lityńska-Dobrzyńska, Lidia; Prażmowski, Mariusz

    2013-08-01

    The microstructure changes and the phase constitution within the layers close to the bonding interface strongly influence the properties of bimetallic strips. In this work, the layers near the interface of explosively welded aluminum and copper plates were investigated by means of microscopic observations, mostly with the use of transmission electron microscopy (TEM) equipped with energy dispersive spectrometry (EDX). The study was focused on the identification of the intermetallic phases, the possible interdiffusion between the copper and the aluminum, and the changes in the dislocation structure of the parent plates. In macro-/mesoscale, the interfaces were outlined by a characteristic sharp transition indicating that there was no mechanical mixing between the welded metals in the solid state. In micro-/nanoscale, the layers adhering to the interface show typical deformed microstructure features, i.e., structure refinement, elongated dislocation cells, slip bands, and microtwins (in copper plate). The internal microstructure of the intermetallic inclusion is composed mostly of dendrites. The electron diffractions and TEM/EDX chemical composition measurements revealed three crystalline equilibrium phases of the γ-Al4Cu9, η-AlCu, and Θ-Al2Cu type (the last one was dominant). However, most of the observed phases of the general Cu m Al n type (also crystalline) do not appear in the equilibrium Al-Cu phase diagram. Inside the intermetallic inclusions, no significant regularity in the phase distribution with respect to the parent sheets was observed. Therefore, it was concluded that the processes occurring in the melt determined their local chemical composition.

  16. Coherent x-ray diffraction imaging of paint pigmentparticles by scanning a phase plate modulator

    SciTech Connect

    Chu Y. S.; Chen B.; Zhang F.; Berenguer F.; Bean R.; Kewish C.; Vila-Comamala J.; Rodenburg J.; Robinson I.

    2011-10-19

    We have implemented a coherent x-ray diffraction imaging technique that scans a phase plate to modulate wave-fronts of the x-ray beam transmitted by samples. The method was applied to measure a decorative alkyd paint containing iron oxide red pigment particles. By employing an iterative algorithm for wave-front modulation phase retrieval, we obtained an image of the paint sample that shows the distribution of the pigment particles and is consistent with the result obtained from a transmission x-ray microscope. The technique has been experimentally proven to be a feasible coherent x-ray imaging method with about 120 nm spatial resolution and was shown to work well with industrially relevant specimens.

  17. Quantitative phase tomography by using x-ray microscope with Foucault knife-edge scanning filter

    NASA Astrophysics Data System (ADS)

    Watanabe, Norio; Tsuburaya, Yuji; Shimada, Akihiro; Aoki, Sadao

    2016-01-01

    Quantitative phase tomography was evaluated by using a differential phase microscope with a Foucault knife-edge scanning filter. A 3D x-ray phase image of polystyrene beads was obtained at 5.4 keV. The reconstructed refractive index was fairly good agreement with the Henke's tabulated data.

  18. Quantitative phase tomography by using x-ray microscope with Foucault knife-edge scanning filter

    SciTech Connect

    Watanabe, Norio; Tsuburaya, Yuji; Shimada, Akihiro; Aoki, Sadao

    2016-01-28

    Quantitative phase tomography was evaluated by using a differential phase microscope with a Foucault knife-edge scanning filter. A 3D x-ray phase image of polystyrene beads was obtained at 5.4 keV. The reconstructed refractive index was fairly good agreement with the Henke’s tabulated data.

  19. Tomographic imaging of transparent biological samples using the pyramid phase microscope.

    PubMed

    Iglesias, Ignacio

    2016-08-01

    We show how a pyramid phase microscope can be used to obtain tomographic information of the spatial variation of refractive index in biological samples using the Radon transform. A method that uses the information provided by the phase microscope for axial and lateral repositioning of the sample when it rotates is also described. Its application to the reconstruction of mouse embryos in the blastocyst stage is demonstrated.

  20. Tomographic imaging of transparent biological samples using the pyramid phase microscope

    PubMed Central

    Iglesias, Ignacio

    2016-01-01

    We show how a pyramid phase microscope can be used to obtain tomographic information of the spatial variation of refractive index in biological samples using the Radon transform. A method that uses the information provided by the phase microscope for axial and lateral repositioning of the sample when it rotates is also described. Its application to the reconstruction of mouse embryos in the blastocyst stage is demonstrated. PMID:27570696

  1. Histomorphometric and electron microscopic analyses of tibial epiphyseal plates from Cosmos 1887 rats

    NASA Technical Reports Server (NTRS)

    Duke, P. J.; Durnova, G.; Montufar-Solis, D.

    1990-01-01

    Previous studies have shown that the changes seen in the bones of growing rats exposed to microgravity are due in part to changes that occur in the growth plate during spaceflight. In this study, growth plates of rats flown aboard Cosmos 1887 (12.5-day flight plus 53.5-h recovery at 1 g) were analyzed using light and electron microscopy and computerized planimetry. The proliferative zone of flight animals was found to be significantly (P less than or equal to 0.01) larger than that of controls, while the reserve and hypertrophic/calcification zones were significantly reduced. Flight animals also had more cells per column in the proliferative zone than did controls and less in the hypertrophic/calcification region. The total number of cells, however, was significantly greater in flight animals. No difference was found in perimeter or in shape factor, but area was significantly less in flight animals. Electron microscopy showed that collagen fibrils in flight animals were wider than in controls. Since the time required for a cell to cycle through the growth plate is 2-3 days at 1 g, the results reported here represent both the effects of exposure to microgravity and the initial stages of recovery from that exposure.

  2. Differential phase microscope and micro-tomography with a Foucault knife-edge scanning filter

    NASA Astrophysics Data System (ADS)

    Watanabe, N.; Hashizume, J.; Goto, M.; Yamaguchi, M.; Tsujimura, T.; Aoki, S.

    2013-10-01

    An x-ray differential phase microscope with a Foucault knife-edge scanning filter was set up at the bending magnet source BL3C, Photon Factory. A reconstructed phase profile from the differential phase image of an aluminium wire at 5.36 keV was fairly good agreement with the numerical simulation. Phase tomography of a biological specimen, such as an Artemia cyst, could be successfully demonstrated.

  3. Quantitative Phase Imaging with a Scanning Transmission X-Ray Microscope

    PubMed Central

    de Jonge, M. D.; Hornberger, B.; Holzner, C.; Legnini, D.; Paterson, D.; McNulty, I.; Jacobsen, C.; Vogt, S.

    2010-01-01

    We obtain quantitative phase reconstructions from differential phase contrast images obtained with a scanning transmission x-ray microscope and 2.5 keV x rays. The theoretical basis of the technique is presented along with measurements and their interpretation. PMID:18518198

  4. Calculation of confocal microscope images of cholesteric blue phases

    NASA Astrophysics Data System (ADS)

    Fukuda, Jun-ichi; Okumura, Yasushi; Kikuchi, Hirotsugu

    2016-03-01

    Real-space images of bulk cholesteric blue phases (BPs) have been successfully obtained by confocal microscopy observations using structural color without doping fluorescent dye. However, theoretical interpretation of these images (for example, the understanding of the relation between intensity distribution and the ordering of BPs) remains challenging because typical lattice spacing of BPs is of the order of the wavelength of visible light, and therefore geometrical optics is entirely useless. In this work, we present a numerical approach to calculate the confocal images of BPs by solving the Maxwell equations. Calculated confocal images are consistent with experimental observations in terms of in-plane symmetry.

  5. Design of an advanced two-phase capillary cold plate

    NASA Technical Reports Server (NTRS)

    Chalmers, D. R.; Kroliczek, E. J.; Ku, J.

    1986-01-01

    The functional principles and implementation of capillary pumped loop (CPL) two phase heat transport system for various elements of the Space Station program are described. Circulation of the working fluid by the surface-tension forces in a fine-pore capillary wick is the core principle of CPL systems. The liquid, usually NH3 at the moment, is changed into a vapor by heat absorption at one end of the loop, and the vapor is carrried back along the wick by the surface tension within the wick. NASA specifications and the results of mechanical and thermal tests for prototype cold plate and the capillary pump designs are outlined. The CPL is targeted for installation on free-flying platforms, attached payloads, and power subsystem thermal control systems.

  6. Development of background reduced Fresnel phase zone plate

    SciTech Connect

    Tamari, Yohei; Azechi, Hiroshi

    2004-10-01

    In study of hot and dense plasma, a high spatial resolution (a few microns) x-ray imaging is very important to observe these plasmas. The Fresnel phase zone plate (FPZP) consists of alternately material and transparent circular annuli placed concentrically, which image x rays using diffraction x rays from all annuli. FPZP have imaged 4.7-4.77 keV x rays with 2.2 {mu}m spatial resolution. However FPZP has a problem that background level is comparable to signal level. In subtraction of background, the error of 10% is caused. For the accurate background subtraction, we designed new FPZP, which consist of three {beta} layers of a transparent zone and two material zones. The new design FPZP parameters (thickness of material zones, each zone width) have been optimized, and in that optimum design signal-to-background ratio is 4 times better than conventional two layers FPZP.

  7. Retrofit implementation of Zernike phase plate imaging for cryo-TEM

    PubMed Central

    Marko, Michael; Leith, ArDean; Hsieh, Chyongere; Danev, Radostin

    2011-01-01

    In-focus phase-plate imaging is particularly beneficial for cryo-TEM because it offers a substantial overall increase in image contrast, without an electron dose penalty, and it simplifies image interpretation. We show how phase-plate cryo-TEM can be implemented with an appropriate existing TEM, and provide a basic practical introduction to use of thin-film (carbon) phase plates. We point out potential pitfalls of phase-plate operation, and discuss solutions. We provide information on evaluating a particular TEM for its suitability. PMID:21272647

  8. Effects of low-spatial-frequency response of phase plates on TEM imaging

    NASA Astrophysics Data System (ADS)

    Edgcombe, C. J.

    2015-10-01

    Images of simple objects produced by a perfect lens and a phase plate have been calculated by use of Abbe theory for Foucault, Hilbert and Zernike phase plates. The results show that with a Zernike plate, white outlines and ringing like those observed previously can be caused by the beam hole, which limits the low-spatial-frequency response of the system even when the lens behaves perfectly. When the change of phase added by the phase plate is distributed over a range of radius rather than a simple step, the unwanted effects are substantially reduced.

  9. High-frequency Pn,Sn phases recorded by ocean bottom seismometers on the Cocos plate

    SciTech Connect

    McCreery, C.S.

    1981-05-01

    Data from ocean bottom seismometers located on the Cocos plate indicate that high-frequency Pn,Sn phases are generated by earthquakes along the subducting margin of that plate and are propagated across the plate. The Sn phase appears to be severely attenuated as it approaches the ridge crest. Estimates of Pn velocity are lower than previous extimates for western Pacific paths, which may indicate a relationship between Pn,Sn velocity and lithospheric age. High frequencies found in these phases suggest that Q for Pn,Sn propagation across the Cocos plate is similar to that for the western Pacific.

  10. Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

    NASA Astrophysics Data System (ADS)

    Patel, Nimit R.; Chhaniwal, Vani K.; Javidi, Bahram; Anand, Arun

    2015-07-01

    Development of devices for automatic identification of diseases is desired especially in developing countries. In the case of malaria, even today the gold standard is the inspection of chemically treated blood smears through a microscope. This requires a trained technician/microscopist to identify the cells in the field of view, with which the labeling chemicals gets attached. Bright field microscopes provide only low contrast 2D images of red blood cells and cell thickness distribution cannot be obtained. Quantitative phase contrast microscopes can provide both intensity and phase profiles of the cells under study. The phase information can be used to determine thickness profile of the cell. Since cell morphology is available, many parameters pertaining to the 3D shape of the cell can be computed. These parameters in turn could be used to decide about the state of health of the cell leading to disease diagnosis. Here the investigations done on digital holographic microscope, which provides quantitative phase images, for comparison of parameters obtained from the 3D shape profile of objects leading to identification of diseased samples is described.

  11. Measurement of relative phase distribution of onion epidermal cells by using the polarization microscope

    NASA Astrophysics Data System (ADS)

    Shin, In Hee; Lee, Ji Yong; Lee, Seungrag; Lee, Dong Ju; Kim, Dug Young

    2007-02-01

    Bio-cells and tissues have intrinsic polarization characteristics, which are changed by external stimulus and internal metamorphosis in cells and tissues and some of the bio-cells and tissues have intrinsic birefringence characteristics, which are also changed by external stimulus and internal metamorphosis in cells and tissues. In this paper, we have developed the polarization microscope for measurement of relative phase which results from birefringence characteristics of materials with improved linear polarizing method and have measured relative phase distribution of onion epidermal cells. From the measurement of the relative phase distribution of onion epidermal cells, decrease of relative phase distribution of onion epidermal cells was investigated as the elapse of time. In decrease of relative phase distribution, relative phase of cell membrane in onion epidermal cells decreased radically as compared with that of cytoplasm because decline of function in cell membrane that takes charge of matter transfer in onion epidermal cells has occurred.

  12. Quantitative photothermal phase imaging of red blood cells using digital holographic photothermal microscope.

    PubMed

    Vasudevan, Srivathsan; Chen, George C K; Lin, Zhiping; Ng, Beng Koon

    2015-05-10

    Photothermal microscopy (PTM), a noninvasive pump-probe high-resolution microscopy, has been applied as a bioimaging tool in many biomedical studies. PTM utilizes a conventional phase contrast microscope to obtain highly resolved photothermal images. However, phase information cannot be extracted from these photothermal images, as they are not quantitative. Moreover, the problem of halos inherent in conventional phase contrast microscopy needs to be tackled. Hence, a digital holographic photothermal microscopy technique is proposed as a solution to obtain quantitative phase images. The proposed technique is demonstrated by extracting phase values of red blood cells from their photothermal images. These phase values can potentially be used to determine the temperature distribution of the photothermal images, which is an important study in live cell monitoring applications.

  13. Change dynamics of RBC morphology after injection glucose for diabetes by diffraction phase microscope

    NASA Astrophysics Data System (ADS)

    Talaykova, N. A.; Kalyanov, A. L.; Lychagov, V. V.; Ryabukho, V. P.; Malinova, L. I.

    2013-11-01

    Experimental setup of diffraction phase microscope (DPM) with double low-coherence lighting system is presented in the paper. Algorithm of interference picture processing and optical thickness, height, volume and mean cells volume (MCV) of RBC calculating is shown. We demonstrate results of experiments with blood smears and ability of the method to calculate 3D model of the biological cells shape. Investigation change dynamics of RBC morphology after injection glucose for diabetes by DPM is shown in the paper.

  14. Digital holographic phase imaging of particles embedded in microscopic structures in three dimensions

    NASA Astrophysics Data System (ADS)

    Park, Jun Yong; Desta, Habben; Maloney, Maxwell C.; Sharikova, Anna; Khmaladze, Alexander

    2016-05-01

    We present a three-dimensional microscopic technique based on digital holographic imaging, which allows highly accurate axial localization of features inside of a three dimensional sample. When a light wave is propagating through, or reflecting from, a microscopic object, the phase changes can be converted into intensity variations using the existing digital microscopic techniques. The phase change indicates the change in the optical path length, which can be then converted to physical thickness, providing the sample height information. This property of holograms is used in phasecontrast techniques, and can also be used for quantitative 3D imaging. However, if the sample contains features with different indices of refraction, this method can only provide the overall optical thickness, and cannot determine where in the axial direction the particular feature is located. As a result, the application of Digital Holographic Microscopy to imaging of organelles within live cells, or defects within semiconductor substrates, is limited to overall morphology of the sample. To determine the axial location of features inside of a three dimensional sample, we developed a phase image processing method based on analyzing images taken from non-zero incident angles. When compared, these images can discriminate between various axial depths of features, while still retaining the information about the overall thickness profile of the sample.

  15. X-ray Phase Imaging Microscopy using a Fresnel Zone Plate and a Transmission Grating

    SciTech Connect

    Yashiro, Wataru; Momose, Atsushi; Takeuchi, Akihisa; Suzuki, Yoshio

    2010-06-23

    We report on a hard X-ray phase imaging microscopy (a phase-difference microscopy) that consists of an objective and a transmission grating. The simple optical system provides a quantitative phase image, and does not need a wave field mostly coherent on the objective. Our method has a spatial resolution almost same as that of the absorption contrast microscope image obtained by removing the grating. We demonstrate how our approach provides a phase image from experimentally obtained images. Our approach is attractive for easily appending a quantitative phase-sensitive mode to normal X-ray microscopes, and has potentially broad applications in biology and material sciences.

  16. Laser scanning confocal microscope with programmable amplitude, phase, and polarization of the illumination beam.

    PubMed

    Boruah, B R; Neil, M A A

    2009-01-01

    We describe the design and construction of a laser scanning confocal microscope with programmable beam forming optics. The amplitude, phase, and polarization of the laser beam used in the microscope can be controlled in real time with the help of a liquid crystal spatial light modulator, acting as a computer generated hologram, in conjunction with a polarizing beam splitter and two right angled prisms assembly. Two scan mirrors, comprising an on-axis fast moving scan mirror for line scanning and an off-axis slow moving scan mirror for frame scanning, configured in a way to minimize the movement of the scanned beam over the pupil plane of the microscope objective, form the XY scan unit. The confocal system, that incorporates the programmable beam forming unit and the scan unit, has been implemented to image in both reflected and fluorescence light from the specimen. Efficiency of the system to programmably generate custom defined vector beams has been demonstrated by generating a bottle structured focal volume, which in fact is the overlap of two cross polarized beams, that can simultaneously improve both the lateral and axial resolutions if used as the de-excitation beam in a stimulated emission depletion confocal microscope.

  17. Phase-gradient contrast in thick tissue with a scanning microscope

    PubMed Central

    Mertz, J.; Gasecka, A.; Daradich, A.; Davison, I.; Coté, D.

    2014-01-01

    It is well known that the principle of reciprocity is valid for light traveling even through scattering or absorptive media. This principle has been used to establish an equivalence between conventional widefield microscopes and scanning microscopes. We make use of this principle to introduce a scanning version of oblique back-illumination microscopy, or sOBM. This technique provides sub-surface phase-gradient and amplitude images from unlabeled tissue, in an epi-detection geometry. That is, it may be applied to arbitrarily thick tissue. sOBM may be implemented as a simple, cost-effective add-on with any scanning microscope, requiring only the availability of an extra input channel in the microscope electronics. We demonstrate here its implementation in combination with two-photon excited fluorescence (TPEF) microscopy and with coherent anti-Stokes Raman scattering (CARS) microscopy, applied to brain or spinal cord tissue imaging. In both cases, sOBM provides information on tissue morphology complementary to TPEF or CARS contrast. This information is obtained simultaneously and is automatically co-registered. Finally, we show that sOBM can be operated at video rate. PMID:24575336

  18. Digital phase-shifting atomic force microscope Moiré method

    NASA Astrophysics Data System (ADS)

    Liu, Chia-Ming; Chen, Lien-Wen

    2005-04-01

    In this study, the digital atomic force microscope (AFM) Moiré method with phase-shifting technology is established to measure the in-plane displacement and strain fields. The Moiré pattern is generated by the interference between the specimen grating and the virtual reference grating formed by digital image processes. The overlapped image is filtered by two-dimensional wavelet transformation to obtain the clear interference Moiré patterns. The four-step phase-shifting method is realized by translating the phase of the virtual reference grating from 0 to 2π. The principle of the digital AFM Moiré method and the phase-shifting technology are described in detail. Experimental results show that this method is convenient to use and efficient in realizing the microscale measurement.

  19. Light-Weight, Single-Phase, Liquid-Cooled Cold Plate (Presentation)

    SciTech Connect

    Narumanchi, S.

    2013-07-01

    This presentation, 'Light-Weight, Low-Cost, Single-Phase Liquid-Cooled Cold Plate,' directly addresses program goals of increased power density, specific power, and lower cost of power electronics components through improved thermal management.

  20. Simple phase-shifting method in a wedge-plate lateral-shearing interferometer.

    PubMed

    Song, Jae Bong; Lee, Yun Woo; Lee, In Won; Lee, Yong-Hee

    2004-07-10

    A simple phase-shifting method in a wedge-plate lateral shearing interferometer is described. Simply moving the wedge plate in an in-plane parallel direction gives the amount of phase shift required for phase-shifting interferometry because the thickness of a wedge plate is not constant and varies along the wedge direction. This method requires only one additional linear translator to move the wedge plate. The required moving distance for a phase shift of the wave front with this method is of the order of a millimeter, whereas the typical moving distance for another method that uses a piezoelectric transducer is of the order of a wavelength. This method yields better precision in controlling the moving distance than do the other methods.

  1. Cryo-EM single particle analysis with the Volta phase plate

    PubMed Central

    Danev, Radostin; Baumeister, Wolfgang

    2016-01-01

    We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required precision. With this approach we obtained a 3.2 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome. The phase plate matches or slightly exceeds the performance of the conventional defocus approach. Spherical aberration becomes a limiting factor for achieving resolutions below 3 Å with in-focus phase plate images. The phase plate could enable single particle analysis of challenging samples in terms of small size, heterogeneity and flexibility that are difficult to solve by the conventional defocus approach. DOI: http://dx.doi.org/10.7554/eLife.13046.001 PMID:26949259

  2. Light-Weight, Low-Cost, Single-Phase, Liquid-Cooled Cold Plate (Presentation)

    SciTech Connect

    Narumanchi, S.

    2013-07-01

    This presentation, 'Light-Weight, Low-Cost, Single-Phase Liquid-Cooled Cold Plate,' directly addresses program goals of increased power density, specific power, and lower cost of power electronics components through improved thermal management.

  3. Characterisation of ferromagnetic rings for Zernike phase plates using the Aharonov-Bohm effect.

    PubMed

    Edgcombe, C J; Ionescu, A; Loudon, J C; Blackburn, A M; Kurebayashi, H; Barnes, C H W

    2012-09-01

    Holographic measurements on magnetised thin-film cobalt rings have demonstrated both onion and vortex states of magnetisation. For a ring in the vortex state, the difference between phases of electron paths that pass through the ring and those that travel outside it was found to agree very well with Aharonov-Bohm theory within measurement error. Thus the magnetic flux in thin-film rings of ferromagnetic material can provide the phase shift required for phase plates in transmission electron microscopy. When a ring of this type is used as a phase plate, scattered electrons will be intercepted over a radial range similar to the ring width. A cobalt ring of thickness 20 nm can produce a phase difference of π/2 from a width of just under 30 nm, suggesting that the range of radial interception for this type of phase plate can be correspondingly small.

  4. Two-phase flow patterns in adiabatic and diabatic corrugated plate gaps

    NASA Astrophysics Data System (ADS)

    Polzin, A.-E.; Kabelac, S.; de Vries, B.

    2016-09-01

    Correlations for two-phase heat transfer and pressure drop can be improved considerably, when they are adapted to specific flow patterns. As plate heat exchangers find increasing application as evaporators and condensers, there is a need for flow pattern maps for corrugated plate gaps. This contribution presents experimental results on flow pattern investigations for such a plate heat exchanger background, using an adiabatic visualisation setup as well as a diabatic setup. Three characteristic flow patterns were observed in the considered range of two-phase flow: bubbly flow, film flow and slug flow. The occurrence of these flow patterns is a function of mass flux, void fraction, fluid properties and plate geometry. Two different plate geometries having a corrugation angle of 27° and 63°, respectively and two different fluids (water/air and R365mfc liquid/vapor) have been analysed. A flow pattern map using the momentum flux is presented.

  5. Chromatic characterization of ion-exchanged glass binary phase plates for mode-division multiplexing.

    PubMed

    Prieto-Blanco, Xesús; Montero-Orille, Carlos; Moreno, Vicente; Mateo, Eduardo F; Liñares, Jesús

    2015-04-10

    Mode-division multiplexing (MDM) in few-mode fibers is regarded as a promising candidate to increase optical network capacity. A fundamental element for MDM is a modal transformer to LP modes which can be implemented in a free-space basis by using multiregion phase plates, that is, LP plates. Likewise, several wavelengths have to be used due to wavelength multiplexing purposes, optical amplification tasks, and so on. In this work we show that efficient monolithic binary phase plates for different wavelengths can be fabricated by ion-exchange in glass and used for MDM tasks. We introduce an optical characterization method of the chromatic properties of such phase plates which combines the inverse Wentzel-Kramers-Brillouin (IWKB) together with Mach-Zehnder and Michelson-based interferometric techniques. The interferometric method provides a measurement of the phase step for several wavelengths, which characterizes the chromatic properties of the phase plate. Consequently, it is shown that the IWKB method allows us to design and characterize the phase plates in an easy and fast way.

  6. An Acoustic Plate Mode Sensor for Biowarfare Toxins, Phase II

    DTIC Science & Technology

    1997-10-01

    Biological agents -- such as bacteria , bacterial toxins and viruses -- must be detected rapidly to allow their neutralization or the quick treatment of...Mode Sensor for Biowarfare Toxins PRINCIPAL INVESTIGATOR: Douglas J. McAllister, Ph.D. CONTRACTING ORGANIZATION: Biode, Incorporated Bangor, Maine...OF PAGES Acoustic Plate Mode, Biowarfare Toxins 54 16. PRICE CODE 17. SECURITY CLASSIFICATION 18. SECURITY CLASSIFICATION 19. SECURITY CLASSIFICATION

  7. 96-well plate-to-plate gravity fluorous solid-phase extraction (F-SPE) for solution-phase library purification.

    PubMed

    Zhang, Wei; Lu, Yimin

    2007-01-01

    Large particle size (125_210 microm) fluorous silica gel bonded with a -SiCH2CH2C8F17 stationary phase has been employed for gravity-driven fluorous solid-phase extraction (F-SPE) on two types of 96-well plates. A 1 or 0.75 g portion of fluorous silica is packed to each well of the 3.5-mL Ex-Blok and the 2.2-mL deep-well filtration plates, respectively. Up to 50 mg of reaction mixture is loaded and then eluted with a fluorophobic solvent (DMSO, DMF, or 85:15 DMF-H2O). Products collected in 96-well receiving plates are directly concentrated on a GeneVac vacuum centrifuge. This simple and highly efficient plate-to-plate F-SPE technique has been demonstrated in the purification of four 96-compound libraries produced by scavenging reactions with 1-(perfluoroctyl)propyl isatoic anhydride (F-IA), amide coupling reactions with 2-chloro-4,6-bis[(perfluorooctyl)propyloxy]-1,3,5-triazine (F-CDMT) or 2,4-dichloro-6-(perfluorooctyl)propyloxy-1,3,5-triazine (F-DCT), and Mitsunobu reactions with fluorous diethyl azodicarboxylate (F-DEAD) and triphenylphosphine (F-TPP). Approximately 80% of products in each library have greater than 85% purity after F-SPE without conducting chromatography.

  8. Two-phase gas-liquid flow characteristics inside a plate heat exchanger

    SciTech Connect

    Nilpueng, Kitti; Wongwises, Somchai

    2010-11-15

    In the present study, the air-water two-phase flow characteristics including flow pattern and pressure drop inside a plate heat exchanger are experimentally investigated. A plate heat exchanger with single pass under the condition of counter flow is operated for the experiment. Three stainless steel commercial plates with a corrugated sinusoidal shape of unsymmetrical chevron angles of 55 and 10 are utilized for the pressure drop measurement. A transparent plate having the same configuration as the stainless steel plates is cast and used as a cover plate in order to observe the flow pattern inside the plate heat exchanger. The air-water mixture flow which is used as a cold stream is tested in vertical downward and upward flow. The results from the present experiment show that the annular-liquid bridge flow pattern appeared in both upward and downward flows. However, the bubbly flow pattern and the slug flow pattern are only found in upward flow and downward flow, respectively. The variation of the water and air velocity has a significant effect on the two-phase pressure drop. Based on the present data, a two-phase multiplier correlation is proposed for practical application. (author)

  9. Programmable phase plate for tool modification in laser machining applications

    DOEpatents

    Thompson Jr., Charles A.; Kartz, Michael W.; Brase, James M.; Pennington, Deanna; Perry, Michael D.

    2004-04-06

    A system for laser machining includes a laser source for propagating a laser beam toward a target location, and a spatial light modulator having individual controllable elements capable of modifying a phase profile of the laser beam to produce a corresponding irradiance pattern on the target location. The system also includes a controller operably connected to the spatial light modulator for controlling the individual controllable elements. By controlling the individual controllable elements, the phase profile of the laser beam may be modified into a desired phase profile so as to produce a corresponding desired irradiance pattern on the target location capable of performing a machining operation on the target location.

  10. Scattering properties of normal and cancerous tissues from human stomach based on phase-contrast microscope

    NASA Astrophysics Data System (ADS)

    Zhang, Hui; Li, Zhifang; Li, Hui

    2012-12-01

    In order to study scattering properties of normal and cancerous tissues from human stomach, we collect images for human gastric specimens by using phase-contrast microscope. The images were processed by the way of mathematics morphology. The equivalent particle size distribution of tissues can be obtained. Combining with Mie scattering theory, the scattering properties of tissues can be calculated. Assume scattering of light in biological tissue can be seen as separate scattering events by different particles, total scattering properties can be equivalent to as scattering sum of particles with different diameters. The results suggest that scattering coefficient of the cancerous tissue is significantly higher than that of normal tissue. The scattering phase function is different especially in the backscattering area. Those are significant clinical benefits to diagnosis cancerous tissue

  11. Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography

    PubMed Central

    1993-01-01

    High voltage electron microscopic tomography was used to determine the organization of the kinetochore plate and its attachment to the underlying chromosome. Six reconstructions were computed from thick sections of Colcemid-treated PtK1 cells and analyzed by a number of computer graphics methods including extensive thin slicing, three- dimensional masking, and volume rendering. When viewed en-face the kinetochore plate appeared to be constructed from a scaffold of numerous 10-20-nm thick fibers or rods. Although the fibers exhibited regions of parallel alignment and hints of a lattice, they were highly variable in length, orientation and spacing. When viewed in stereo, groups of these fibers were often seen oriented in different directions at different depths to give an overall matted appearance to the structure. When viewed "on edge," the plate was 35-40 nm thick, and in thin slices many regions were tripartite with electron-opaque domains, separated by a more translucent middle layer, forming the inner and outer plate boundaries. These domains were joined at irregular intervals. In some slices, each domain appeared as a linear array of 10- 20-nm dots or rods embedded in a less electron-opaque matrix, and adjacent dots within or between domains often appeared fused to form larger blocks. The plate was connected to the underlying chromosome by less densely arrayed 10-20-nm thick fibers that contacted the chromosome-facing (i.e., inner) surface of the plate in numerous patches. These patches tended to be arrayed in parallel rows perpendicular to the long axis of the chromosome. In contrast to connecting fibers, corona fibers were more uniformly distributed over the cytoplasmic-facing (i.e., outer) surface of the plate. When large portions of the reconstructions were viewed, either en-face or in successive slices parallel to the long axis of the chromosome, the edges of the plate appeared splayed into multiple "fingers" that partly encircled the primary constriction

  12. Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography.

    PubMed

    McEwen, B F; Arena, J T; Frank, J; Rieder, C L

    1993-01-01

    High voltage electron microscopic tomography was used to determine the organization of the kinetochore plate and its attachment to the underlying chromosome. Six reconstructions were computed from thick sections of Colcemid-treated PtK1 cells and analyzed by a number of computer graphics methods including extensive thin slicing, three-dimensional masking, and volume rendering. When viewed en-face the kinetochore plate appeared to be constructed from a scaffold of numerous 10-20-nm thick fibers or rods. Although the fibers exhibited regions of parallel alignment and hints of a lattice, they were highly variable in length, orientation and spacing. When viewed in stereo, groups of these fibers were often seen oriented in different directions at different depths to give an overall matted appearance to the structure. When viewed "on edge," the plate was 35-40 nm thick, and in thin slices many regions were tripartite with electron-opaque domains, separated by a more translucent middle layer, forming the inner and outer plate boundaries. These domains were joined at irregular intervals. In some slices, each domain appeared as a linear array of 10-20-nm dots or rods embedded in a less electron-opaque matrix, and adjacent dots within or between domains often appeared fused to form larger blocks. The plate was connected to the underlying chromosome by less densely arrayed 10-20-nm thick fibers that contacted the chromosome-facing (i.e., inner) surface of the plate in numerous patches. These patches tended to be arrayed in parallel rows perpendicular to the long axis of the chromosome. In contrast to connecting fibers, corona fibers were more uniformly distributed over the cytoplasmic-facing (i.e., outer) surface of the plate. When large portions of the reconstructions were viewed, either en-face or in successive slices parallel to the long axis of the chromosome, the edges of the plate appeared splayed into multiple "fingers" that partly encircled the primary constriction

  13. Fabrication of a new substrate for atomic force microscopic observation of DNA molecules from an ultrasmooth sapphire plate.

    PubMed

    Yoshida, K; Yoshimoto, M; Sasaki, K; Ohnishi, T; Ushiki, T; Hitomi, J; Yamamoto, S; Sigeno, M

    1998-04-01

    A new stable substrate applicable to the observation of DNA molecules by atomic force microscopy (AFM) was fabricated from a ultrasmooth sapphire (alpha-Al2O3 single crystal) plate. The atomically ultrasmooth sapphire as obtained by high-temperature annealing has hydrophobic surfaces and could not be used for the AFM observation of DNA. However, sapphire treated with Na3PO4 aqueous solution exhibited a hydrophilic character while maintaining a smooth surface structure. The surface of the wet-treated sapphire was found by x-ray photoelectron spectroscopy and AFM to be approximately 0.3 nm. The hydrophilic surface character of the ultrasmooth sapphire plate made it easy for DNA molecules to adhere to the plate. Circular molecules of the plasmid DNA could be imaged by AFM on the hydrophilic ultrasmooth sapphire plate.

  14. Fabrication of a new substrate for atomic force microscopic observation of DNA molecules from an ultrasmooth sapphire plate.

    PubMed Central

    Yoshida, K; Yoshimoto, M; Sasaki, K; Ohnishi, T; Ushiki, T; Hitomi, J; Yamamoto, S; Sigeno, M

    1998-01-01

    A new stable substrate applicable to the observation of DNA molecules by atomic force microscopy (AFM) was fabricated from a ultrasmooth sapphire (alpha-Al2O3 single crystal) plate. The atomically ultrasmooth sapphire as obtained by high-temperature annealing has hydrophobic surfaces and could not be used for the AFM observation of DNA. However, sapphire treated with Na3PO4 aqueous solution exhibited a hydrophilic character while maintaining a smooth surface structure. The surface of the wet-treated sapphire was found by x-ray photoelectron spectroscopy and AFM to be approximately 0.3 nm. The hydrophilic surface character of the ultrasmooth sapphire plate made it easy for DNA molecules to adhere to the plate. Circular molecules of the plasmid DNA could be imaged by AFM on the hydrophilic ultrasmooth sapphire plate. PMID:9545030

  15. Measurement of displacement using phase shifted wedge plate lateral shearing interferometry

    NASA Astrophysics Data System (ADS)

    Disawal, Reena; Prakash, Shashi

    2016-03-01

    In present communication, a simple technique for measurement of displacement using phase shifted wedge plate lateral shearing interferometry is described. The light beam from laser is expanded and illuminates a wedge plate of relatively large angle. Light transmitted through the wedge plate is converged onto a reflecting specimen using a focusing lens. Back-reflected wavefront from the specimen is incident on the wedge plate. Because of the tilt and shear of the wavefront reflected from the wedge plate, typical straight line fringes appear. These fringes are superimposed onto a sinusoidal grating forming a moiré pattern. The orientation of the moiré fringes is a function of specimen displacement. Four step phase shifting test procedure has been incorporated by translating the grating in phase steps of π/2. Necessary mathematical formulation to establish correlation between the 'difference phase' and the displacement of the specimen surface is undertaken. The technique is automatic and provides resolution and expanded uncertainty of 1 μm and 0.246 μm, respectively. Detailed uncertainty analysis is also reported.

  16. The Biological Sensor for Detection of Bacterial Cells in Liquid Phase Based on Plate Acoustic Wave

    NASA Astrophysics Data System (ADS)

    Borodina, Irina; Zaitsev, Boris; Shikhabudinov, Alexander; Guliy, Olga; Ignatov, Oleg; Teplykh, Andrey

    The interactions "bacterial cells - bacteriophages", "bacterial cells - antibodies" and "bacterial cells - mini- antibodies" directly in liquid phase were experimentally investigated with a help of acoustic sensor. The acoustic sensor under study represents two-channel delay line based on the plate of Y-X lithium niobate. One channel of delay line was electrically shorted, the second channel was electrically open. The liquid container was glued on plate surface between transducers of delay line. The dependencies of the change in phase and insertion loss on concentration of bacteriophages, antibodies, and mini- antibodies were obtained for both channels of delay line.

  17. Volta phase plate cryo-EM of the small protein complex Prx3

    NASA Astrophysics Data System (ADS)

    Khoshouei, Maryam; Radjainia, Mazdak; Phillips, Amy J.; Gerrard, Juliet A.; Mitra, Alok K.; Plitzko, Jürgen M.; Baumeister, Wolfgang; Danev, Radostin

    2016-01-01

    Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

  18. Motion detection and pattern tracking in microscopical images using phase correlation approach

    NASA Astrophysics Data System (ADS)

    Gladilin, Evgeny; Kappel, Constantin; Eils, Roland

    2007-03-01

    High-throughput live-cell imaging is one of the important tools for the investigation of cellular structure and functions in modern experimental biology. Automatic processing of time series of microscopic images is hampered by a number of technical and natural factors such as permanent movements of cells in the optical field, alteration of optical cell appearance and high level of noise. Detection and compensation of global motion of groups of cells or relocation of a single cell within a dynamical multi-cell environment is the first indispensable step in the image analysis chain. This article presents an approach for detection of global image motion and single cell tracking in time series of confocal laser scanning microscopy images using an extended Fourier-phase correlation technique, which allows for analysis of non-uniform multi-body motion in partially-similar images. Our experimental results have shown that the developed approach is capable to perform cell tracking and registration in dynamical and noisy scenes, and provides a robust tool for fully-automatic registration of time-series of microscopic images.

  19. Phase-contrast versus off-axis illumination: is a more complex microscope always more powerful?

    PubMed

    Hostounský, Zdenek; Pelc, Radek

    2007-06-01

    In this article, a practical demonstration suitable for any biology college classroom is presented. With the examples of a complex biological specimen (slug's radula) and a simple reference specimen (electron microscopical grid imprint in gelatin), both of which can be easily prepared, the capabilities of two imaging modes commonly used in optical microscopy are demonstrated. The results obtained under phase contrast (a rather sophisticated method, 1953 Nobel Prize to Zernike) and off-axis illumination (a very simple method) are compared. The off-axis illumination setup is capable of delivering noticeably better microscopic images of these two particular specimens, yet it can be easily assembled in a laboratory classroom. The outcome of such a demonstration is expected to be the realization on the part of the students that one needs to carefully choose the apparatus to address a given biological problem, with the "bottom line" being that a more complex one may not necessarily yield better results. An attempt to explain this "paradox" is presented, in the particular case presented here, partly from the physiology of vision perspective (the shape-from-shading problem). The overall aim of the present article is to induce in students critical thinking about the capabilities of a laboratory equipment in general and about data interpretation.

  20. Phase Velocity Method for Guided Wave Measurements in Composite Plates

    NASA Astrophysics Data System (ADS)

    Moreno, E.; Galarza, N.; Rubio, B.; Otero, J. A.

    Carbon Fiber Reinforced Polymer is a well-recognized material for aeronautic applications. Its plane structure has been widely used where anisotropic characteristics should be evaluated with flaw detection. A phase velocity method of ultrasonic guided waves based on a pitch-catch configuration is presented for this purpose. Both shear vertical (SV) and shear horizontal (SH) have been studied. For SV (Lamb waves) the measurements were done at different frequencies in order to evaluate the geometrical dispersion and elastic constants. The results for SV are discussed with an orthotropic elastic model. Finally experiments with lamination flaws are presented.

  1. Quantum coherent optical phase modulation in an ultrafast transmission electron microscope.

    PubMed

    Feist, Armin; Echternkamp, Katharina E; Schauss, Jakob; Yalunin, Sergey V; Schäfer, Sascha; Ropers, Claus

    2015-05-14

    Coherent manipulation of quantum systems with light is expected to be a cornerstone of future information and communication technology, including quantum computation and cryptography. The transfer of an optical phase onto a quantum wavefunction is a defining aspect of coherent interactions and forms the basis of quantum state preparation, synchronization and metrology. Light-phase-modulated electron states near atoms and molecules are essential for the techniques of attosecond science, including the generation of extreme-ultraviolet pulses and orbital tomography. In contrast, the quantum-coherent phase-modulation of energetic free-electron beams has not been demonstrated, although it promises direct access to ultrafast imaging and spectroscopy with tailored electron pulses on the attosecond scale. Here we demonstrate the coherent quantum state manipulation of free-electron populations in an electron microscope beam. We employ the interaction of ultrashort electron pulses with optical near-fields to induce Rabi oscillations in the populations of electron momentum states, observed as a function of the optical driving field. Excellent agreement with the scaling of an equal-Rabi multilevel quantum ladder is obtained, representing the observation of a light-driven 'quantum walk' coherently reshaping electron density in momentum space. We note that, after the interaction, the optically generated superposition of momentum states evolves into a train of attosecond electron pulses. Our results reveal the potential of quantum control for the precision structuring of electron densities, with possible applications ranging from ultrafast electron spectroscopy and microscopy to accelerator science and free-electron lasers.

  2. Quantum coherent optical phase modulation in an ultrafast transmission electron microscope

    NASA Astrophysics Data System (ADS)

    Feist, Armin; Echternkamp, Katharina E.; Schauss, Jakob; Yalunin, Sergey V.; Schäfer, Sascha; Ropers, Claus

    2015-05-01

    Coherent manipulation of quantum systems with light is expected to be a cornerstone of future information and communication technology, including quantum computation and cryptography. The transfer of an optical phase onto a quantum wavefunction is a defining aspect of coherent interactions and forms the basis of quantum state preparation, synchronization and metrology. Light-phase-modulated electron states near atoms and molecules are essential for the techniques of attosecond science, including the generation of extreme-ultraviolet pulses and orbital tomography. In contrast, the quantum-coherent phase-modulation of energetic free-electron beams has not been demonstrated, although it promises direct access to ultrafast imaging and spectroscopy with tailored electron pulses on the attosecond scale. Here we demonstrate the coherent quantum state manipulation of free-electron populations in an electron microscope beam. We employ the interaction of ultrashort electron pulses with optical near-fields to induce Rabi oscillations in the populations of electron momentum states, observed as a function of the optical driving field. Excellent agreement with the scaling of an equal-Rabi multilevel quantum ladder is obtained, representing the observation of a light-driven `quantum walk' coherently reshaping electron density in momentum space. We note that, after the interaction, the optically generated superposition of momentum states evolves into a train of attosecond electron pulses. Our results reveal the potential of quantum control for the precision structuring of electron densities, with possible applications ranging from ultrafast electron spectroscopy and microscopy to accelerator science and free-electron lasers.

  3. Wavefront-guided correction of ocular aberrations: Are phase plate and refractive surgery solutions equal?

    NASA Astrophysics Data System (ADS)

    Marchese, Linda E.; Munger, Rejean; Priest, David

    2005-08-01

    Wavefront-guided laser eye surgery has been recently introduced and holds the promise of correcting not only defocus and astigmatism in patients but also higher-order aberrations. Research is just beginning on the implementation of wavefront-guided methods in optical solutions, such as phase-plate-based spectacles, as alternatives to surgery. We investigate the theoretical differences between the implementation of wavefront-guided surgical and phase plate corrections. The residual aberrations of 43 model eyes are calculated after simulated refractive surgery and also after a phase plate is placed in front of the untreated eye. In each case, the current wavefront-guided paradigm that applies a direct map of the ocular aberrations to the correction zone is used. The simulation results demonstrate that an ablation map that is a Zernike fit of a direct transform of the ocular wavefront phase error is not as efficient in correcting refractive errors of sphere, cylinder, spherical aberration, and coma as when the same Zernike coefficients are applied to a phase plate, with statistically significant improvements from 2% to 6%.

  4. Design and test of a pumped two-phase mounting plate. [for spacecraft thermal control systems

    NASA Technical Reports Server (NTRS)

    Grote, M. G.; Swanson, T. D.

    1985-01-01

    The design, fabrication, and testing of the full-scale development unit of a pumped two-phase mounting plate (TPMP) used in advanced two-phase spacecraft thermal control systems are described. The mounting plate is tested with R-11 in the evaporator mode for total heat loads of over 3000 watts and local heat fluxes over 4 W/sq cm, and in the condenser mode with condenser loads from 60 to 400 watts and inlet qualities from 8 to 94 percent. The calculated heat-transfer coefficients are between 0.66 and 1.0 W/sq cm/C and are nearly independent of the flow rate and heat load except at very low heat loads. It is shown that the TPMP can be run with inlet conditions down to 22 C subcooling without any significant gradients in the plate and that it performs well with nonuniform heat fluxes.

  5. An automatic system for measurement of retardation of wave plates based on phase-shifted method

    NASA Astrophysics Data System (ADS)

    Gao, Zhishan; Yan, Ming

    2005-02-01

    A practical system is described to measure the retardation of wave plates with phase-shifted method. The tested wave plate is put in and the original angle between the axis of it and the analyzer is random, not 45 degree. For the measurement is made rapidly and automatically, a standard wave plate act as a compensator, the stepping motor is used to drive the analyzer to realize phase shifting and a grating encoder is used to measure its rotating angle. At the same time, while the beam comes out from the analyzer, the photoelectric detector gets its intensity, and then the signals is magnified, filtered and sent to computer through its serial port. The results show the system has the advantages of costing little time and high accuracy.

  6. Biaxial nematic phase stability and demixing behaviour in monolayers of rod-plate mixtures.

    PubMed

    Martínez-Ratón, Yuri; González-Pinto, Miguel; Velasco, Enrique

    2016-09-21

    We theoretically study the phase behaviour of monolayers of hard rod-plate mixtures using a fundamental-measure density functional in the restricted-orientation (Zwanzig) approximation. Particles can rotate in 3D but their centres of mass are constrained to be on a flat surface. In addition, we consider both species to be subject to an attractive potential proportional to the particle contact area on the surface and with adsorption strengths that depend on the species type. Particles have board-like shape, with sizes chosen using a symmetry criterion: same volume and same aspect ratio κ. Phase diagrams were calculated for κ = 10, 20 and 40 and different values of adsorption strengths. For small adsorption strengths the mixtures exhibit a second-order uniaxial nematic-biaxial nematic transition for molar fraction of rods 0 ≤x≲ 0.9. In the uniaxial nematic phase the particle axes of rods and plates are aligned perpendicular and parallel to the monolayer, respectively. At the transition, the orientational symmetry of the plate axes is broken, and they orient parallel to a director lying on the surface. For large and equal adsorption strengths the mixture demixes at low pressures into a uniaxial nematic phase, rich in plates, and a biaxial nematic phase, rich in rods. The demixing transition is located between two tricritical points. Also, at higher pressures and in the plate-rich part of the phase diagram, the system exhibits a strong first-order uniaxial nematic-biaxial nematic phase transition with a large density coexistence gap. When rod adsorption is considerably large while that of plates is small, the transition to the biaxial nematic phase is always of second order, and its region of stability in the phase diagram considerably widens. At very high pressures the mixture can effectively be identified as a two-dimensional mixture of squares and rectangles which again demixes above a certain critical point. We also studied the relative stability of uniform

  7. Magnetorheological Finishing for Imprinting Continuous Phase Plate Structure onto Optical Surfaces

    SciTech Connect

    Menapace, J A; Dixit, S N; Genin, F Y; Brocious, W F

    2004-01-05

    Magnetorheological finishing (MRF) techniques have been developed to manufacture continuous phase plates (CPP's) and custom phase corrective structures on polished fused silica surfaces. These phase structures are important for laser applications requiring precise manipulation and control of beam-shape, energy distribution, and wavefront profile. The MRF's unique deterministic-sub-aperture polishing characteristics make it possible to imprint complex topographical information onto optical surfaces at spatial scale-lengths approaching 1 mm. In this study, we present the results of experiments and model calculations that explore imprinting two-dimensional sinusoidal structures. Results show how the MRF removal function impacts and limits imprint fidelity and what must be done to arrive at a high quality surface. We also present several examples of this imprinting technology for fabrication of phase correction plates and CPPs for use at high fluences.

  8. Planar gas chromatography column on aluminum plate with multi-walled carbon nanotubes as stationary phase

    NASA Astrophysics Data System (ADS)

    Platonov, I. A.; Platonov, V. I.; Pavelyev, V. S.

    2016-04-01

    The high selectivity of the adsorption layer for low-boiling alkanes is shown, the separation factor (α) couple iso-butane / butane is 1.9 at a column temperature of 50 °C.The paper presents sorption and selective properties of planar gas chromatography column on aluminum plate with multi-walled carbon nanotubes as the stationary phase.

  9. Diffraction of a plane, finite-radius wave by a spiral phase plate.

    PubMed

    Kotlyar, V V; Khonina, S N; Kovalev, A A; Soifer, V A; Elfstrom, H; Turunen, J

    2006-06-01

    We derive analytical expressions containing a hypergeometric function to describe the Fresnel and Fraunhofer diffraction of a plane wave of circular and ringlike cross section by a spiral phase plate (SPP) of an arbitrary integer order. Experimental diffraction patterns generated by an SPP fabricated in resist through direct e-beam writing are in good agreement with the theoretical intensity distribution.

  10. A compact "water-window" microscope with 60-nm spatial resolution based on a double stream gas-puff target and Fresnel zone plate optics

    NASA Astrophysics Data System (ADS)

    Wachulak, Przemyslaw; Torrisi, Alfio; Nawaz, Muhammad F.; Adjei, Daniel; Bartnik, Andrzej; Kostecki, Jerzy; Wegrzynski, Łukasz; Vondrová, Šárka; Turňová, Jana; Fok, Tomasz; Jančarek, Alexandr; Fiedorowicz, Henryk

    2015-05-01

    Radiation with shorter illumination wavelength allows for extension of the diffraction limit towards nanometer scale, which is a straightforward way to significantly improve a spatial resolution in photon based microscopes. Soft X-ray (SXR) radiation, from the so called "water window" spectral range, λ=2.3-4.4 nm, which is particularly suitable for biological imaging due to natural optical contrast, providing much better spatial resolution than one obtained with visible light microscopes. The high contrast is obtained because of selective absorption of radiation by carbon and water, being constituents of the biological samples. We present a desk-top system, capable of resolving 60 nm features in few seconds exposure time. We exploit the advantages of a compact, laser-plasma SXR source, based on a double stream nitrogen gas puff target, developed at the Institute of Optoelectronics, Military University of Technology. The source, emitting quasi-monochromatic, incoherent radiation, in the "water widow" spectral range at λ = 2.88 nm, is coupled with ellipsoidal, grazing incidence condenser and Fresnel zone plate objective. The construction of the microscope with some recent images of test and real samples will be presented and discussed.

  11. Application of phase stretch transform to plate license identification under blur and noise conditions (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Asghari, Hossein; Hadar, Ofer; Jalali, Bahram

    2016-09-01

    This paper deals with implementing a new algorithm for edge detection based on the Phase Stretch Transform (PST) for purposes of car plate license recognition. In PST edge detection algorithm, the image is first filtered with a spatial kernel followed by application of a nonlinear frequency-dependent phase. The output of the transform is the phase in the spatial domain. The main step is the 2-D phase function which is typically applied in the frequency domain. The amount of phase applied to the image is frequency dependent with higher amount of phase applied to higher frequency features of the image. Since sharp transitions, such as edges and corners, contain higher frequencies, PST emphasizes the edge information. Features can be further enhanced by applying thresholding and morphological operations. Here we investigate the influence of noise and blur on the ability to recognize the characters in the plate license, by comparison of our suggested algorithm with the well known Canny algorithm. We use several types of noise distributions among them, Gaussian noise, salt and paper noise and uniform distributed noise, with several levels of noise variances. The simulated blur is related to the car velocity and we applied several filters representing different velocities of the car. Another interesting degradation that we intend to investigate is the cases that Laser shield license plate cover is used to distort the image taken by the authorities. Our comparison results are presented in terms of True positive, False positive and False negative probabilities.

  12. Single-Phase Drive Ultrasonic Linear Motor Using a Linked Twin Square Plate Vibrator

    NASA Astrophysics Data System (ADS)

    Yokoyama, Keiji; Tamura, Hideki; Masuda, Kentaro; Takano, Takehiro

    2013-07-01

    A novel linear motion ultrasonic motor, which uses a single resonance mode driven by a single phase and has the same motor characteristics for operation in reverse directions, is developed. An in-plane breathing mode in the square plate is strongly driven by the transverse effect of a piezoelectric ceramic. A stator resonator consists of twin square plates linked by V-shaped beams. Only one side of the square plate can be excited by the resonance of the breathing mode, when the other passive side plate is electrically opened so that the effective elasticities and the resonant frequencies between both plates are different; as a result, the friction edge of the resonator vibrates in a slant locus to move a load slider. The reverse operation is easily obtained by switching the driving side of the square plates. We designed the stator resonator by FEM analysis and fabricated a prototype for our experiment. The prototype motor showed good characteristics, for example, a moving slider velocity of 100 mm/s, a thrust force of 3.5 N, and an efficiency of 30% when the preload was 10 N, the input effective voltage was 5 V, and the input power was 1.2 W.

  13. Method for preparing surfaces of metal composites having a brittle phase for plating. [Patent application

    DOEpatents

    Coates, C.W.; Wilson, T.J.

    1982-05-19

    The present invention is directed to a method for preparing surfaces of two-phase metal composites having relatively brittle and malleable components for plating with corrosion-resistant material. In practice of the present invention, the surfaces of the composite are etched to remove a major portion or fraction of the brittle component. The etched surface is then peened with particulates for breaking the brittle component from the surfaces and for spreading or smearing the malleable component over the surfaces. The peened surface is then chemically cleaned of residual traces of the brittle component to which the corrosion-resistant material may be plated thereon in an adherent manner.

  14. Combined spectral estimator for phase velocities of multimode Lamb waves in multilayer plates.

    PubMed

    Ta, De-an; Liu, Zhen-qing; Liu, Xiao

    2006-12-22

    A novel combined spectral estimate (CSE) method for differentiation and estimation the phase velocities of multimode Lamb waves whose wave numbers are much close or overlap one another in multiplayer plates is presented in this paper, which based on auto-regressive (AR) model and 2-D FFT. Simulated signals in brass plate were processed by 2-D FFT and CSE. And experiments are performed by using two conventional angle probes as emitter and receiver on the same surface of three-layered aluminum/xpoxy/aluminum plates, which include symmetrical and unsymmetrical plates. The multimode Lamb waves are excited in these laminates, and the received signal is processed by 2-D FFT and CSE, respectively. The results showed that the phase velocities of multimode signals whose wave numbers are much closed cannot be differentiated by 2-D FFT, but CSE has strong spatial resolution. Compared the measured phase velocities with the theoretical values, the error is smaller than 2% on the whole. It promises to be a useful method in experimental signals processing of multimode Lamb waves.

  15. Influence of Two-Phase Thermocapillary Flow on Liquid Retention in Microscopic Pores

    NASA Technical Reports Server (NTRS)

    Schmidt, George R.; Nadarajah, Arun; Chung, T. J.; Karr, Gerald R.

    1995-01-01

    An important feature of screened propellant acquisition devices is the retention capability or maximum maintainable pressure difference across the porous barrier separating the liquid and gas. Previous experiments with liquid hydrogen showed a marked reduction in retention when the tank containing the device was pressurized with hydrogen vapor. These tests, however did not indicate any appreciable degradation in retention with helium pressurization or direct heating through the screen. The objective of this article is to determine if the thermocapillary convection arising from phase change in the microscopic pores of such screens could cause these disparities in performance. A numerical model of flow in a single pore suggests that the thermocapillary-induced gradient in liquid pressure along the surface can strongly affect surface morphology. In an evaporative environment, this gradient exerts a stabilizing influence on surface curvature, and preserves the momentum balance between the liquid and gas. With condensation, it causes a force imbalance and a destabilizing suction in the middle of the pore that reduces retention. Results also indicate that introducing an inert gas, such as helium, suppresses this retention loss mechanism by lowering thermocapillary circulation and its associated interfacial pressure gradient.

  16. Direct-write liquid phase transformations with a scanning transmission electron microscope

    DOE PAGES

    Unocic, Raymond R.; Lupini, Andrew R.; Borisevich, Albina Y.; ...

    2016-08-03

    The highly energetic electron beam from a scanning transmission electron microscope (STEM) can induce local changes in the state of matter, ranging from local knock-out and atomic movement, to amorphization/crystallization, and chemical/electrochemical reactions occuring at localized liquid-solid and gas-solid interfaces. To date, fundamental studies of e-beam induced phenomena and practical applications have been limited by conventional e-beam rastering modes that allow only for uniform e-beam exposures. Here we develop an automated liquid phase nanolithography method that is capable of directly writing nanometer scaled features within silicon nitride encapsulated liquid cells. An external beam control system, connected to the scan coilsmore » of an aberration-corrected STEM, is used to precisely control the position, dwell time, and scan velocity of a sub-nanometer STEM probe. Site-specific locations in a sealed liquid cell containing an aqueous solution of H2PdCl4 are irradiated to controllably deposit palladium onto silicon nitride membranes. We determine the threshold electron dose required for the radiolytic deposition of metallic palladium, explore the influence of electron dose on the feature size and morphology of nanolithographically patterned nanostructures, and propose a feedback-controlled monitoring method for active control of the nanofabricated structures through STEM detector signal monitoring. As a result, this approach enables both fundamental studies of electron beam induced interactions with matter, as well as opens a pathway to fabricate nanostructures with tailored architectures and chemistries via shape-controlled nanolithographic patterning from liquid phase precursors.« less

  17. Direct-write liquid phase transformations with a scanning transmission electron microscope

    SciTech Connect

    Unocic, Raymond R.; Lupini, Andrew R.; Borisevich, Albina Y.; Cullen, David A.; Kalinin, Sergei V.; Jesse, Stephen

    2016-08-03

    The highly energetic electron beam from a scanning transmission electron microscope (STEM) can induce local changes in the state of matter, ranging from local knock-out and atomic movement, to amorphization/crystallization, and chemical/electrochemical reactions occuring at localized liquid-solid and gas-solid interfaces. To date, fundamental studies of e-beam induced phenomena and practical applications have been limited by conventional e-beam rastering modes that allow only for uniform e-beam exposures. Here we develop an automated liquid phase nanolithography method that is capable of directly writing nanometer scaled features within silicon nitride encapsulated liquid cells. An external beam control system, connected to the scan coils of an aberration-corrected STEM, is used to precisely control the position, dwell time, and scan velocity of a sub-nanometer STEM probe. Site-specific locations in a sealed liquid cell containing an aqueous solution of H2PdCl4 are irradiated to controllably deposit palladium onto silicon nitride membranes. We determine the threshold electron dose required for the radiolytic deposition of metallic palladium, explore the influence of electron dose on the feature size and morphology of nanolithographically patterned nanostructures, and propose a feedback-controlled monitoring method for active control of the nanofabricated structures through STEM detector signal monitoring. As a result, this approach enables both fundamental studies of electron beam induced interactions with matter, as well as opens a pathway to fabricate nanostructures with tailored architectures and chemistries via shape-controlled nanolithographic patterning from liquid phase precursors.

  18. A real-time pseudocolor encoding technique for the phase rate-of-change in the imaging system of microscope

    NASA Astrophysics Data System (ADS)

    Chen, Guanying; Duan, Wenshan

    1999-05-01

    A real-time white light stereo pseudocolor encoding technique for the phase rate-of-change in the imaging system of microscope is presented. It is analyzed by the theory of partially coherent light. The analytic results showed that the information about phase rate-of-change of the input object function is reflected in the output stereo pseudocolor image under given conditions and illuminated by incoherent source.

  19. Toward structural/chemical cotailoring of phase-change Ge-Sb-Te in a transmission electron microscope.

    PubMed

    Zhang, W; Kim, J-G; Zheng, W T; Cui, X Q; Kim, Y-J; Song, S A

    2015-03-01

    Ge2Sb2Te5, as the prototype material for phase-change memory, can be transformed from amorphous phase into nanoscale rocksalt-type GeTe provided with an electron irradiation assisted by heating to 520°C in a 1250 kV transmission electron microscope. This sheds a new light into structural and chemical cotailoring of materials through coupling of thermal and electrical fields.

  20. Switchable Ultrathin Quarter-wave Plate in Terahertz Using Active Phase-change Metasurface

    PubMed Central

    Wang, Dacheng; Zhang, Lingchao; Gu, Yinghong; Mehmood, M. Q.; Gong, Yandong; Srivastava, Amar; Jian, Linke; Venkatesan, T.; Qiu, Cheng-Wei; Hong, Minghui

    2015-01-01

    Metamaterials open up various exotic means to control electromagnetic waves and among them polarization manipulations with metamaterials have attracted intense attention. As of today, static responses of resonators in metamaterials lead to a narrow-band and single-function operation. Extension of the working frequency relies on multilayer metamaterials or different unit cells, which hinder the development of ultra-compact optical systems. In this work, we demonstrate a switchable ultrathin terahertz quarter-wave plate by hybridizing a phase change material, vanadium dioxide (VO2), with a metasurface. Before the phase transition, VO2 behaves as a semiconductor and the metasurface operates as a quarter-wave plate at 0.468 THz. After the transition to metal phase, the quarter-wave plate operates at 0.502 THz. At the corresponding operating frequencies, the metasurface converts a linearly polarized light into a circularly polarized light. This work reveals the feasibility to realize tunable/active and extremely low-profile polarization manipulation devices in the terahertz regime through the incorporation of such phase-change metasurfaces, enabling novel applications of ultrathin terahertz meta-devices. PMID:26442614

  1. Tandem-Phase Zone-Plate Optics for High-Energy X-ray Focusing

    NASA Astrophysics Data System (ADS)

    Kagoshima, Yasushi; Takano, Hidekazu; Koyama, Takahisa; Tsusaka, Yoshiyuki; Saikubo, Akihiko

    2011-02-01

    An optical system consisting of two phase zone plates closely arranged in tandem was constructed for focusing high-energy X-rays. The phase zone plates were made from tantalum and their combined thickness was 4.8 µm. An ideal diffraction efficiency of 30% is expected at 30 keV, which is about 3 times higher than that of a single zone plate. The focusing properties at 30 keV were studied both numerically and experimentally. The coaxial tandem arrangement was precisely achieved by observing Young's interference patterns in the far-field produced by the two point foci. A focus size of ˜4 µm was obtained. The photon flux density was 2.2 ×1013 photons/s/mm2, which is 2.4 and 85 times higher than that obtained with a single zone plate and without focusing, respectively. The focused beam was used for scanning X-ray fluorescence microscopy and the residual tin distribution on a float glass surface was imaged.

  2. Axisymmetric deformation of plates and shells with phase trasformations under thermal cycling

    NASA Astrophysics Data System (ADS)

    Shkutin, L. I.

    2008-03-01

    A mathematical formulation is given of nonlinear axisymmetric buckling problems for plates and shells in the two-phase zones of austenite-to-martensite transformation. Numerical solutions of the direct-and inverse-transformation problems are used to construct hysteresis loops for thermomechanically cycled, pressure-loaded circular plates and shallow spherical domes of titanium nickelide (NiTi) alloy. It is shown that dynamic instability of the dome deformation process can occur during transformation under loads notably lower than the upper critical values for the isothermal states of the material outside the transformation zone. A theoretical analysis gives external loads below which the dome remains stable in the thermally cycled material with phase transformations.

  3. Fraunhofer diffraction of the plane wave by a multilevel (quantized) spiral phase plate.

    PubMed

    Kotlyar, Victor V; Kovalev, Alexey A

    2008-01-15

    We obtain an analytical expression in the form of a finite sum of plane waves that describes the paraxial scalar Fraunhofer diffraction of a limited plane wave by a multilevel (quantized) spiral phase plate (SPP) bounded by a polygonal aperture. For several topological charges of the SPP we numerically obtain the minimal number of SPP sectors for which the RMS between the Fraunhofer diffraction patterns for multilevel and continuous SPP does not exceed 2%.

  4. Surface modes in "photonic cholesteric liquid crystal-phase plate-metal" structure.

    PubMed

    Vetrov, S Ya; Pyatnov, M V; Timofeev, I V

    2014-05-01

    The light transmission spectrum has been calculated for a "cholesteric liquid crystal-phase plate-metal" structure. It is shown that the system can have an isolated waveguide surface mode with characteristics efficiently controllable by external fields acting on the cholesteric. The degree of localization of surface modes and the transmission coefficients have been found to differ considerably for the light of different polarizations.

  5. Insight into the microscopic structure of an AdS black hole from a thermodynamical phase transition.

    PubMed

    Wei, Shao-Wen; Liu, Yu-Xiao

    2015-09-11

    Comparing with an ordinary thermodynamic system, we investigate the possible microscopic structure of a charged anti-de Sitter black hole completely from the thermodynamic viewpoint. The number density of the black hole molecules is introduced to measure the microscopic degrees of freedom of the black hole. We found that the number density suffers a sudden change accompanied by a latent heat when the black hole system crosses the small-large black hole coexistence curve, while when the system passes the critical point, it encounters a second-order phase transition with a vanishing latent heat due to the continuous change of the number density. Moreover, the thermodynamic scalar curvature suggests that there is a weak attractive interaction between two black hole molecules. These phenomena might cast new insight into the underlying microscopic structure of a charged anti-de Sitter black hole.

  6. Diffraction theory of high numerical aperture subwavelength circular binary phase Fresnel zone plate.

    PubMed

    Zhang, Yaoju; An, Hongchang; Zhang, Dong; Cui, Guihua; Ruan, Xiukai

    2014-11-03

    An analytical model of vector formalism is proposed to investigate the diffraction of high numerical aperture subwavelength circular binary phase Fresnel zone plate (FZP). In the proposed model, the scattering on the FZP's surface, reflection and refraction within groove zones are considered and diffraction fields are calculated using the vector Rayleigh-Sommerfeld integral. The numerical results obtained by the proposed phase thick FZP (TFZP) model show a good agreement with those obtained by the finite-difference time-domain (FDTD) method within the effective extent of etch depth. The optimal etch depths predicted by both methods are approximately equal. The analytical TFZP model is very useful for designing a phase and hybrid amplitude-phase FZP with high-NA and short focal length.

  7. Evidence of lower-mantle slab penetration phases in plate motions.

    PubMed

    Goes, Saskia; Capitanio, Fabio A; Morra, Gabriele

    2008-02-21

    It is well accepted that subduction of the cold lithosphere is a crucial component of the Earth's plate tectonic style of mantle convection. But whether and how subducting plates penetrate into the lower mantle is the subject of continuing debate, which has substantial implications for the chemical and thermal evolution of the mantle. Here we identify lower-mantle slab penetration events by comparing Cenozoic plate motions at the Earth's main subduction zones with motions predicted by fully dynamic models of the upper-mantle phase of subduction, driven solely by downgoing plate density. Whereas subduction of older, intrinsically denser, lithosphere occurs at rates consistent with the model, younger lithosphere (of ages less than about 60 Myr) often subducts up to two times faster, while trench motions are very low. We conclude that the most likely explanation is that older lithosphere, subducting under significant trench retreat, tends to lie down flat above the transition to the high-viscosity lower mantle, whereas younger lithosphere, which is less able to drive trench retreat and deforms more readily, buckles and thickens. Slab thickening enhances buoyancy (volume times density) and thereby Stokes sinking velocity, thus facilitating fast lower-mantle penetration. Such an interpretation is consistent with seismic images of the distribution of subducted material in upper and lower mantle. Thus we identify a direct expression of time-dependent flow between the upper and lower mantle.

  8. Three-dimensional phase-contrast X-ray microtomography with scanning-imaging X-ray microscope optics.

    PubMed

    Takeuchi, Akihisa; Uesugi, Kentaro; Suzuki, Yoshio

    2013-09-01

    A three-dimensional (3D) X-ray tomographic micro-imaging system has been developed. The optical system is based on a scanning-imaging X-ray microscope (SIXM) optics, which is a hybrid system consisting of a scanning microscope optics with a one-dimensional (1D) focusing (line-focusing) device and an imaging microscope optics with a 1D objective. In the SIXM system, each 1D dataset of a two-dimensional (2D) image is recorded independently. An object is illuminated with a line-focused beam. Positional information of the region illuminated by the line-focused beam is recorded with the 1D imaging microscope optics as line-profile data. By scanning the object with the line focus, 2D image data are obtained. In the same manner as for a scanning microscope optics with a multi-pixel detector, imaging modes such as phase contrast and absorption contrast can be arbitrarily configured after the image data acquisition. By combining a tomographic scan method and the SIXM system, quantitative 3D imaging is performed. Results of a feasibility study of the SIXM for 3D imaging are shown.

  9. The Cell Cycle: An Activity Using Paper Plates to Represent Time Spent in Phases of the Cell Cycle

    ERIC Educational Resources Information Center

    Scherer, Yvette D.

    2014-01-01

    In this activity, students are given the opportunity to combine skills in math and geometry for a biology lesson in the cell cycle. Students utilize the data they collect and analyze from an online onion-root-tip activity to create a paper-plate time clock representing a 24-hour cell cycle. By dividing the paper plate into appropriate phases of…

  10. Method for preparing surfaces of metal composites having a brittle phase for plating

    DOEpatents

    Coates, Cameron W.; Wilson, Thomas J.

    1984-01-01

    The present invention is directed to a method for preparing surfaces of two-phase metal composites having relatively brittle and malleable components for plating with corrosion-resistant material. In practice of the present invention, the surfaces of the composites are etched to remove a major portion or fraction of the brittle component. The etched surface is then peened with particulates for breaking the brittle component from the surfaces and for spreading or smearing the malleable component over the surfaces. The peened surface is then chemically cleaned of residual traces of the brittle component so as to provide a surface of essentially the malleable component to which the corrosion-resistant material may be plated thereon in an adherent manner.

  11. Low-cost, broadband static phase plate for generating atmosphericlike turbulence.

    PubMed

    Rhoadarmer, T A; Angel, J R

    2001-06-20

    Over the past decade adaptive optics (AO) has proved its worth as AO systems have been used successfully on several telescopes to improve image resolution. As scientists and engineers push the technological state of the art in an effort to make bigger, faster, and better systems, it has become more and more important to test and verify the operation of these systems in a controlled laboratory setting. To perform full-system tests in the presence of atmospheric turbulence, some sort of turbulence generator is needed. We describe a simple, low-cost approach to making static phase plates that generate atmosphericlike wave-front aberrations. These plates have several advantages over traditional heated-air turbulence generators and, as such, are better suited for well-controlled, detailed testing of an AO system.

  12. Method and apparatus for acoustic plate mode liquid-solid phase transition detection

    DOEpatents

    Blair, Dianna S.; Freye, Gregory C.; Hughes, Robert C.; Martin, Stephen J.; Ricco, Antonio J.

    1993-01-01

    A method and apparatus for sensing a liquid-solid phase transition event is provided which comprises an acoustic plate mode detecting element placed in contact with a liquid or solid material which generates a high-frequency acoustic wave that is attenuated to an extent based on the physical state of the material is contact with the detecting element. The attenuation caused by the material in contact with the acoustic plate mode detecting element is used to determine the physical state of the material being detected. The method and device are particularly suited for detecting conditions such as the icing and deicing of wings of an aircraft. In another aspect of the present invention, a method is provided wherein the adhesion of a solid material to the detecting element can be measured using the apparatus of the invention.

  13. Method and apparatus for acoustic plate mode liquid-solid phase transition detection

    NASA Astrophysics Data System (ADS)

    Blair, D. S.; Frye, G. C.; Hughes, R. C.; Martin, S. J.; Ricco, A. J.

    1990-05-01

    A method and apparatus for sensing a liquid-solid phase transition event is provided which comprises an acoustic plate mode detecting element placed in contact with a liquid or solid material which generates a high-frequency acoustic wave that is attenuated to an extent based on the physical state of the material in contact with the detecting element. The attenuation caused by the material in contact with the acoustic plate mode detecting element is used to determine the physical state of the material being detected. The method and device are particularly suited for detecting conditions such as the icing and deicing of wings of an aircraft. In another aspect of the present invention, a method is provided wherein the adhesion of a solid material to the detecting element can be measured using the apparatus of the invention.

  14. Phase equilibria and plate-fluid interfacial tensions for associating hard sphere fluids confined in slit pores.

    PubMed

    Fu, Dong; Li, Xiao-Sen

    2006-08-28

    The excess Helmholtz free energy functional for associating hard sphere fluid is formulated by using a modified fundamental measure theory [Y. X. Yu and J. Z. Wu, J. Chem. Phys. 117, 10156 (2002)]. Within the framework of density functional theory, the thermodynamic properties including phase equilibria for both molecules and monomers, equilibrium plate-fluid interfacial tensions and isotherms of excess adsorption, average molecule density, average monomer density, and plate-fluid interfacial tension for four-site associating hard sphere fluids confined in slit pores are investigated. The phase equilibria inside the hard slit pores and attractive slit pores are determined according to the requirement that temperature, chemical potential, and grand potential in coexistence phases should be equal and the plate-fluid interfacial tensions at equilibrium states are predicted consequently. The influences of association energy, fluid-solid interaction, and pore width on phase equilibria and equilibrium plate-fluid interfacial tensions are discussed.

  15. Generation of cylindrical vector beams based on common-path interferometer with a vortex phase plate

    NASA Astrophysics Data System (ADS)

    Liang, Yansheng; Yan, Shaohui; Yao, Baoli; Lei, Ming; Min, Junwei; Yu, Xianghua

    2016-04-01

    Cylindrical vector (CV) beams have found increasing applications in physics, biology, and chemistry. To generate CV beams, interferometric technique is popularly adopted due to its flexibility. However, most interferometric configurations for the generation of CV beams are faced with system instability arising from external disturbance, limiting their practical applications. A common-path interferometer for the generation of radially and azimuthally polarized beams is proposed to improve the system stability. The optical configuration consists of a vortex phase plate acting to tailor the phase profile and a cube nonpolarizing beamsplitter to split the input beam into two components with mirror-like spiral phase distribution. The generated CV beams show a high quality in polarization and exhibit a better stability of beam profile than those obtained by noncommon-path interferometric configurations.

  16. Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

    NASA Astrophysics Data System (ADS)

    Bon, Pierre; Aknoun, Shérazade; Savatier, Julien; Wattellier, Benoit; Monneret, Serge

    2013-02-01

    In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.

  17. Phase-Contrast versus Off-Axis Illumination: Is a More Complex Microscope Always More Powerful?

    ERIC Educational Resources Information Center

    Hostounsky, Zdenek; Pelc, Radek

    2007-01-01

    In this article, a practical demonstration suitable for any biology college classroom is presented. With the examples of a complex biological specimen (slug's radula) and a simple reference specimen (electron microscopical grid imprint in gelatin), both of which can be easily prepared, the capabilities of two imaging modes commonly used in optical…

  18. Using the Volta phase plate with defocus for cryo-EM single particle analysis

    PubMed Central

    Danev, Radostin; Tegunov, Dimitry; Baumeister, Wolfgang

    2017-01-01

    Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å. DOI: http://dx.doi.org/10.7554/eLife.23006.001 PMID:28109158

  19. Study of polarization properties of fiber-optics probes with use of a binary phase plate.

    PubMed

    Alferov, S V; Khonina, S N; Karpeev, S V

    2014-04-01

    We conduct a theoretical and experimental study of the distribution of the electric field components in the sharp focal domain when rotating a zone plate with a π-phase jump placed in the focused beam. Comparing the theoretical and experimental results for several kinds of near-field probes, an analysis of the polarization sensitivity of different types of metal-coated aperture probes is conducted. It is demonstrated that with increasing diameter of the non-metal-coated tip part there occurs an essential redistribution of sensitivity in favor of the transverse electric field components and an increase of the probe's energy throughput.

  20. Subtractive two-frame three-beam phase-stepping interferometry for testing surface shape of quasi-parallel plates.

    PubMed

    Sunderland, Zofia; Patorski, Krzysztof; Trusiak, Maciej

    2016-12-26

    We present an effective method of testing the surface shape of quasi-parallel plates which requires only two phase-shifted three-beam interferograms. We derive a general formula for difference of two three-beam interferograms as a function of the phase shift value. The phase shift does not have to be precisely determined and uniform in the image domain. We show and compare results of extracting the fringe set and corresponding phase distribution related to the plate front surface shape using the two dimensional continuous wavelet transform, Hilbert-Huang transform and Fourier transform methods. Simulated and experimental data is used to verify the algorithm performance and robustness.

  1. Inexpensive read-out for coincident electron spectroscopy with a transmission electron microscope at nanometer scale using micro channel plates and multistrip anodes

    NASA Astrophysics Data System (ADS)

    Hollander, R. W.; Bom, V. R.; van Eijk, C. W. E.; Faber, J. S.; Hoevers, H.; Kruit, P.

    1994-09-01

    The elemental composition of a sample at nanometer scale is determined by measurement of the characteristic energy of Auger electrons, emitted in coincidence with incoming primary electrons from a microbeam in a scanning transmission electron microscope (STEM). Single electrons are detected with position sensitive detectors, consisting of MicroChannel Plates (MCP) and MultiStrip Anodes (MSA), one for the energy of the Auger electrons (Auger-detector) and one for the energy loss of primary electrons (EELS-detector). The MSAs are sensed with LeCroy 2735DC preamplifiers. The fast readout is based on LeCroy's PCOS III system. On the detection of a coincidence (Event) energy data of Auger and EELS are combined with timing data to an Event word. Event words are stored in list mode in a VME memory module. Blocks of Event words are scanned by transputers in VME and two-dimensional energy histograms are filled using the timing information to obtain a maximal true/accidental ratio. The resulting histograms are stored on disk of a PC-386, which also controls data taking. The system is designed to handle 10 5 Events per second, 90% of which are accidental. In the histograms the "true" to "accidental" ratio will be 5. The dead time is 15%.

  2. Highly intense monocycle terahertz vortex generation by utilizing a Tsurupica spiral phase plate

    PubMed Central

    Miyamoto, Katsuhiko; Kang, Bong Joo; Kim, Won Tae; Sasaki, Yuta; Niinomi, Hiromasa; Suizu, Koji; Rotermund, Fabian; Omatsu, Takashige

    2016-01-01

    Optical vortex, possessing an annular intensity profile and an orbital angular momentum (characterized by an integer termed a topological charge) associated with a helical wavefront, has attracted great attention for diverse applications due to its unique properties. In particular for terahertz (THz) frequency range, several approaches for THz vortex generation, including molded phase plates consisting of metal slit antennas, achromatic polarization elements and binary-diffractive optical elements, have been recently proposed, however, they are typically designed for a specific frequency. Here, we demonstrate highly intense broadband monocycle vortex generation near 0.6 THz by utilizing a polymeric Tsurupica spiral phase plate in combination with tilted-pulse-front optical rectification in a prism-cut LiNbO3 crystal. A maximum peak power of 2.3 MW was obtained for THz vortex output with an expected topological charge of 1.15. Furthermore, we applied the highly intense THz vortex beam for studying unique nonlinear behaviors in bilayer graphene towards the development of nonlinear super-resolution THz microscopy and imaging system. PMID:27966595

  3. Focal length measurement based on Fresnel diffraction from a phase plate.

    PubMed

    Dashtdar, Masoomeh; Mohammad-Ali Hosseini-Saber, S

    2016-09-10

    A method based on the Fresnel diffraction of light from the phase step is introduced for measuring effective focal length (EFL) and back focal length (BFL) of optical imaging systems. It is shown that, as a transparent plane-parallel plate is illuminated at a boundary region by a monochromatic beam of light, Fresnel diffraction occurs because of the abrupt change in phase imposed by the finite change in refractive index at the plate boundary. Variation of the incident angle in a convergent (or divergent) beam of light causes the periodic intensity along the central fringe of the diffraction pattern. The measurement of the extrema position of the intensity distribution accurately provides the EFL and BFL. The technique is easy to apply and can measure a wide range of both positive and negative focal lengths. The measuring setup can be very compact with low mechanical and optical noises. As examples of this technique, the EFLs of five different lenses are experimentally obtained. The results are quite consistent with the values indicated by the lens manufacturer.

  4. Highly intense monocycle terahertz vortex generation by utilizing a Tsurupica spiral phase plate

    NASA Astrophysics Data System (ADS)

    Miyamoto, Katsuhiko; Kang, Bong Joo; Kim, Won Tae; Sasaki, Yuta; Niinomi, Hiromasa; Suizu, Koji; Rotermund, Fabian; Omatsu, Takashige

    2016-12-01

    Optical vortex, possessing an annular intensity profile and an orbital angular momentum (characterized by an integer termed a topological charge) associated with a helical wavefront, has attracted great attention for diverse applications due to its unique properties. In particular for terahertz (THz) frequency range, several approaches for THz vortex generation, including molded phase plates consisting of metal slit antennas, achromatic polarization elements and binary-diffractive optical elements, have been recently proposed, however, they are typically designed for a specific frequency. Here, we demonstrate highly intense broadband monocycle vortex generation near 0.6 THz by utilizing a polymeric Tsurupica spiral phase plate in combination with tilted-pulse-front optical rectification in a prism-cut LiNbO3 crystal. A maximum peak power of 2.3 MW was obtained for THz vortex output with an expected topological charge of 1.15. Furthermore, we applied the highly intense THz vortex beam for studying unique nonlinear behaviors in bilayer graphene towards the development of nonlinear super-resolution THz microscopy and imaging system.

  5. Diffraction of plane waves by finite-radius spiral phase plates of integer and fractional topological charge.

    PubMed

    Garcia-Gracia, Hipolito; Gutiérrez-Vega, Julio C

    2009-04-01

    A detailed analysis of the plane-wave diffraction by a finite-radius circular spiral phase plate (SPP) with integer and fractional topological charge and with variable transmission coefficients inside and outside of the plate edge is presented. We characterize the effect of varying the transmission coefficients and the parameters of the SPP on the propagated field. The vortex structure for integer and fractional phase step of the SPPs with and without phase apodization at the plate edge is also analyzed. The consideration of the interference between the light crossing the SPP and the light that undergoes no phase alteration at the aperture plane reveals new and interesting phenomena associated to this classical problem.

  6. An alternative view of phospholipid phase behavior at the air-water interface. Microscope and film balance studies.

    PubMed Central

    von Tscharner, V; McConnell, H M

    1981-01-01

    Pure-lipid films at the water interface have surface-pressures vs. area isotherms that are often interpreted as involving first-order phase transitions from a condensed region to a liquid-expanded region. Two phases are presumed to coexist in the intermediate part of the isotherm. We constructed a film balance that could be placed on the stage of an epifluorescence microscope. A dipalmitoyl phosphatidylcholine film containing a low concentration of a fluorescent lipid probe showed an inhomogeneous fluorescence distribution in the so-called liquid-expanded region of the isotherm. Only the intermediate and condensed regions could be prepared so as to be optically homogeneous below 25 degrees C. We investigated membrane flow and lateral lipid diffusion in the membrane on the trough. The isotherms and isochores were measured. The results require, at least, a modified description of the monolayer structure in various regions of the isotherms. The solid-condensed region corresponds to a gel phase of the lipids where there is no flow in the membrane, lateral diffusion is low, the compressibility is low, and the membrane is optically homogeneous. The "liquid-condensed/liquid-expanded" region appears to be a homogeneous membrane where lateral diffusion and membrane flow are both rapid. This is a region of high compressibility. The "liquid-expanded" region is not homogeneous as seen under the microscope, and the flow of the surface layer can be very fast. PMID:6895478

  7. Noncontact Atomic Force Microscope Dissipation Reveals a Central Peak of SrTiO_{3} Structural Phase Transition.

    PubMed

    Kisiel, M; Pellegrini, F; Santoro, G E; Samadashvili, M; Pawlak, R; Benassi, A; Gysin, U; Buzio, R; Gerbi, A; Meyer, E; Tosatti, E

    2015-07-24

    The critical fluctuations at second order structural transitions in a bulk crystal may affect the dissipation of mechanical probes even if completely external to the crystal surface. Here, we show that noncontact force microscope dissipation bears clear evidence of the antiferrodistortive phase transition of SrTiO_{3}, known for a long time to exhibit a unique, extremely narrow neutron scattering "central peak." The noncontact geometry suggests a central peak linear response coupling connected with strain. The detailed temperature dependence reveals for the first time the intrinsic central peak width of order 80 kHz, 2 orders of magnitude below the established neutron upper bound.

  8. Microscopic Modeling of Tribochemical Processes Vapor-Phase Lubrication and Nanotribology in MEMS

    DTIC Science & Technology

    2003-05-01

    INJECTORS, NAVIER STOKES EQUATIONS , JET FLOW, ADHESIVES, FLUID FLOW, LIQUID JETS, MOLECULAR DYNAMICS....VAPOR PHASES, *LUBRICATION, *TRIBOLOGY, * NANOTECHNOLOGY , *MICROELECTROMECHANICAL SYSTEMS, SURFACE ROUGHNESS, ELECTROMECHANICAL DEVICES, NOZZLES

  9. Fabrication of large-scale multilevel phase-type Fresnel zone plate arrays by femtosecond laser direct writing

    NASA Astrophysics Data System (ADS)

    Yu, Yan-Hao; Tian, Zhen-Nan; Jiang, Tong; Niu, Li-Gang; Gao, Bing-Rong

    2016-03-01

    We report on the fabrication of large-scale eight-level phase-type Fresnel zone plate arrays (FZPAs) by femtosecond-laser direct writing technology. A high-speed galvanometer scanning system was used to fabricate each Fresnel zone plate to realize high fabrication efficiency. To overcome the limited fabrication scale in the case of galvanometer scanning, inter-plate movements were controlled by multi-axis air-bearing precise positioning stages. With the system, FZPAs whose fill-factor was designed to be 100% realized a diffraction efficiency of 89%. The focusing and imaging properties of the FZPAs were also evaluated, and the FZPAs showed high fidelity.

  10. Microscopic description of octupole shape-phase transitions in light actinide and rare-earth nuclei

    NASA Astrophysics Data System (ADS)

    Nomura, K.; Vretenar, D.; Nikšić, T.; Lu, Bing-Nan

    2014-02-01

    A systematic analysis of low-lying quadrupole and octupole collective states is presented based on the microscopic energy density functional framework. By mapping the deformation constrained self-consistent axially symmetric mean-field energy surfaces onto the equivalent Hamiltonian of the sdf interacting boson model (IBM), that is, onto the energy expectation value in the boson condensate state, the Hamiltonian parameters are determined. The study is based on the global relativistic energy density functional DD-PC1. The resulting IBM Hamiltonian is used to calculate excitation spectra and transition rates for the positive- and negative-parity collective states in four isotopic chains characteristic for two regions of octupole deformation and collectivity: Th, Ra, Sm, and Ba. Consistent with the empirical trend, the microscopic calculation based on the systematics of β2-β3 energy maps, the resulting low-lying negative-parity bands and transition rates show evidence of a shape transition between stable octupole deformation and octupole vibrations characteristic for β3-soft potentials.

  11. Macroscopic and microscopic investigations on uniaxial ratchetting of two-phase Ti–6Al–4V alloy

    SciTech Connect

    Kang, Guozheng; Dong, Yawei; Liu, Yujie; Jiang, Han

    2014-06-01

    The uniaxial ratchetting of Ti–6Al–4V alloy with two phases (i.e., primary hexagonal close packed (HCP) α and secondary body-centered cubic (BCC) β phases) was investigated by macroscopic and microscopic experiments at room temperature. Firstly, the effects of cyclic softening/hardening feature, applied mean stress and stress amplitude on the uniaxial ratchetting of the alloy were discussed. The macroscopic investigation of Ti–6Al–4V alloy presents obvious strain-amplitude-dependent cyclic softening, as well as a three-staged evolution curve with regard to the ratchetting strain rate. The ratchetting depends greatly on the applied mean stress and stress amplitude while the ratchetting strain increases with the increasing applied mean stress and stress amplitude. Then, the evolution of dislocation patterns and deformation twinning during the uniaxial ratchetting of two-phase Ti–6Al–4V alloy were observed using transmission electron microscopy (TEM). The microscopic observation shows that deformation twinning occurs in the primary α phase and its amount increases gradually during the uniaxial ratchetting. Simultaneously, the planar dislocation evolves from discrete lines to some dislocation nets and parallel lines with the increasing number of cycles. The deformation twinning in the primary α phase is one of main contributions to the uniaxial ratchetting of Ti–6Al–4V alloy, and should be considered in the construction of corresponding constitutive model. - Highlights: • A three-staged ratchetting occurs in the stress-controlled cyclic tests of Ti–6Al–4V alloy. • Dislocation patterns change from discrete lines to nets and parallel lines. • Deformation twinning occurs during the uniaxial ratchetting. • Both dislocation slipping and twinning are the causes of ratchetting.

  12. A novel silver-coated solid-phase microextraction metal fiber based on electroless plating technique.

    PubMed

    Feng, Juanjuan; Sun, Min; Li, Jubai; Liu, Xia; Jiang, Shengxiang

    2011-09-09

    A novel silver-coated solid-phase microextraction fiber was prepared based on electroless plating technique. Good extraction performance of the fiber for model compounds including phthalate esters (dibutyl phthalate, dioctyl phthalate, dicyclohexyl phthalate and diallyl phthalate) and polycyclic aromatic hydrocarbons (naphthalene, fluorene, phenanthrene, fluoranthene) in aqueous solution was obtained. Under the optimized conditions (extraction temperature, extraction time, ionic strength and desorption temperature), the proposed SPME-GC method showed wide linear ranges with correlation coefficients (R(2)) ranging from 0.9745 to 0.9984. The limits of detection were at the range of 0.02 to 0.1 μg L(-1). Single fiber repeatability and fiber-to-fiber reproducibility as well as stability to acid, alkali and high temperature were studied and the results were all satisfactory. The method was applied successfully to the aqueous extracts of disposable paper cup and instant noodle barrel. Several kinds of analytes were detected and quantified.

  13. Cascades of pi-phase plates: a transparent diffractive focusing system.

    PubMed

    Cagniot, Emmanuel; Fromager, Michael; Godin, Thomas; Traïche, Mohamed; Passilly, Nicolas; Päivänranta, Birgit; Ait-Ameur, Kamel

    2010-07-01

    Typically, refractive lenses are used to focus rays of light, but an alternative way can be found by exploiting diffraction of light. It is well known that cascades of hard-edge apertures are able to focus light but with the great drawback of absorption losses. In this paper, we demonstrate that replacing hard-edge apertures with pi-phase plates within a cascade greatly improves the focusing of collimated Gaussian beams. In addition, we propose a simple model to design this cascade, in particular to find the locations and the radii of the different optics once the focal length has been chosen. This model deduced from numerical simulation is useful for sizing cascades consisting of a high number of components and characterized by a strong focusing ability, without requiring a time-consuming optimization process.

  14. SEARCHING FOR PLANETS IN HOLEY DEBRIS DISKS WITH THE APODIZING PHASE PLATE

    SciTech Connect

    Meshkat, Tiffany; Kenworthy, Matthew A.; Bailey, Vanessa P.; Su, Kate Y. L.; Hinz, Philip M.; Smith, Paul S.; Mamajek, Eric E.

    2015-02-10

    We present our first results from a high-contrast imaging search for planetary mass companions around stars with gapped debris disks, as inferred from the stars' bright infrared excesses. For the six considered stars, we model the disks' unresolved infrared spectral energy distributions in order to derive the temperature and location of the disk components. With VLT/NaCo Apodizing Phase Plate coronagraphic L'-band imaging, we search for planetary mass companions that may be sculpting the disks. We detect neither disks nor companions in this sample, confirmed by comparing plausible point sources with archival data. In order to calculate our mass sensitivity limit, we revisit the stellar age estimates. One target, HD 17848, at 540 ± 100 Myr old is significantly older than previously estimated. We then discuss our high-contrast imaging results with respect to the disk properties.

  15. Test results of a pumped two-phase mounting plate with ammonia. [designed for spacecraft thermal control

    NASA Technical Reports Server (NTRS)

    Swanson, T. D.; Mccabe, M. E., Jr.; Grote, M. G.

    1987-01-01

    The design, fabrication, and testing of full-scale prototype units of a two-phase mounting plate (TPMP), which will be used in a two-phase ammonia-based thermal control system for a large spacecraft, are described. The mounting plate uses an evaporator design in which liquid is mechanically pumped through porous feed tubes within the plate. The prototype TPMPs were tested with ammonia at heat loads over 3000 W (3.2 W/sq cm) and local heat fluxes of up to 4 W/sq cm. Calculated total heat transfer coefficients from these tests were between 0.8 and 1.0 W/sq cm per C. This represents a better than twenty-fold improvement over comparable single-phase heat transfer coefficients. Design diagrams are included.

  16. Macroscopic rates, microscopic observations, and molecular models of the dissolution of carbonate phases.

    SciTech Connect

    Duckworth, Owen W.; Cygan, Randall Timothy; Martin, Scot T.

    2004-05-01

    Bulk and surface energies are calculated for endmembers of the isostructural rhombohedral carbonate mineral family, including Ca, Cd, Co, Fe, Mg, Mn, Ni, and Zn compositions. The calculations for the bulk agree with the densities, bond distances, bond angles, and lattice enthalpies reported in the literature. The calculated energies also correlate with measured dissolution rates: the lattice energies show a log-linear relationship to the macroscopic dissolution rates at circumneutral pH. Moreover, the energies of ion pairs translated along surface steps are calculated and found to predict experimentally observed microscopic step retreat velocities. Finally, pit formation excess energies decrease with increasing pit size, which is consistent with the nonlinear dissolution kinetics hypothesized for the initial stages of pit formation.

  17. MicroRaman, PXRD, EDS and microscopic investigation of magnesium calcite biomineral phases. The case of sea urchin biominerals

    NASA Astrophysics Data System (ADS)

    Borzęcka-Prokop, B.; Wesełucha-Birczyńska, A.; Koszowska, E.

    2007-02-01

    This study concerns Mg-calcite characterization (and in particular molecular structure and microstructural studies of mineral phases) of a sea urchin mineralised test and spines. Sea urchins are spiny sea animals (kingdom Animalia, phylum Echinodermata, class Echinoidea). Microscopic observations, SEM, EDS, PXRD and spectroscopic microRaman methods have been applied to characterize the biomineral parts of the sea urchin. The latter technique is very useful in research of biological systems and especially suitable for monitoring differences within biomineral phases exhibiting varieties of morphological forms. Crystalline magnesium calcium carbonate, Mg xCa 1- xCO 3 (magnesian calcite; space group R-3 cH; a = 4.9594(8) Å, c = 16.886(6) Å), has been identified as the predominant biomineral component.

  18. Nonanalytic microscopic phase transitions and temperature oscillations in the microcanonical ensemble: An exactly solvable one-dimensional model for evaporation

    NASA Astrophysics Data System (ADS)

    Hilbert, Stefan; Dunkel, Jörn

    2006-07-01

    We calculate exactly both the microcanonical and canonical thermodynamic functions (TDFs) for a one-dimensional model system with piecewise constant Lennard-Jones type pair interactions. In the case of an isolated N -particle system, the microcanonical TDFs exhibit (N-1) singular (nonanalytic) microscopic phase transitions of the formal order N/2 , separating N energetically different evaporation (dissociation) states. In a suitably designed evaporation experiment, these types of phase transitions should manifest themselves in the form of pressure and temperature oscillations, indicating cooling by evaporation. In the presence of a heat bath (thermostat), such oscillations are absent, but the canonical heat capacity shows a characteristic peak, indicating the temperature-induced dissociation of the one-dimensional chain. The distribution of complex zeros of the canonical partition may be used to identify different degrees of dissociation in the canonical ensemble.

  19. Thin-Film Phase Plates for Transmission Electron Microscopy Fabricated from Metallic Glasses.

    PubMed

    Dries, Manuel; Hettler, Simon; Schulze, Tina; Send, Winfried; Müller, Erich; Schneider, Reinhard; Gerthsen, Dagmar; Luo, Yuansu; Samwer, Konrad

    2016-10-01

    Thin-film phase plates (PPs) have become an interesting tool to enhance the contrast of weak-phase objects in transmission electron microscopy (TEM). The thin film usually consists of amorphous carbon, which suffers from quick degeneration under the intense electron-beam illumination. Recent investigations have focused on the search for alternative materials with an improved material stability. This work presents thin-film PPs fabricated from metallic glass alloys, which are characterized by a high electrical conductivity and an amorphous structure. Thin films of the zirconium-based alloy Zr65.0Al7.5Cu27.5 (ZAC) were fabricated and their phase-shifting properties were evaluated. The ZAC film was investigated by different TEM techniques, which reveal beneficial properties compared with amorphous carbon PPs. Particularly favorable is the small probability for inelastic plasmon scattering, which results from the combined effect of a moderate inelastic mean free path and a reduced film thickness due to a high mean inner potential. Small probability plasmon scattering improves contrast transfer at high spatial frequencies, which makes the ZAC alloy a promising material for PP fabrication.

  20. Coherence-controlled holographic microscopy principle embodiment into Q-PHASE microscope: story of a successful technology transfer

    NASA Astrophysics Data System (ADS)

    Lostak, M.; Chmelik, R.

    2016-03-01

    Curiously, the coherence-controlled holographic microscopy (CCHM) was brought into the world owing to the endeavor of Chmelik's team at Brno University of Technology (BUT) to avoid scanning in confocal microscopy. As coherence gating seemed to be the way, the Leith & Upatnieks proposal of incoherent holography had been considered attractive. Their method made interference system free from strict dependence on both spatial and temporal coherence. Off axis holographic system proposed on such basis has been proved capable of coherence based depth discrimination in single wide-field shot in reflected-light arrangement. Consequently, extremely low-coherence holographic imaging had been found highly contributive also to the image quality depriving it from coherence artefacts and improving its transversal resolution. This is why CCHM promised high precision of quantitative phase imaging (QPI) in transmitted light set up that was realized for cell biology. However the cost of necessarily complicated optical design and need of very precise mechanics forced the team of prof Chmelik at BUT to search for a company capable of mastering the instrument. It was TESCAN ORSAY the highly successful scanning electron microscopes producer that finally took charge of the commercial design. Long-term collaboration of the company with BUT made possible both the CCHM technology successful transfer up to Q-PHASE microscope production as well as the company Light microscopy division reinforcement. This contribution merges views of CCHM technology author and the TESCAN development team.

  1. Estimation of visibility of phase contrast with extraction voltages for field emission gun electron microscopes.

    PubMed

    Meng, Xing

    2017-02-01

    Estimation was made for visibility of phase contrast with varying extraction voltages. The resulting decay rates of visibility show that images with low image contrast from cryo EM will be seriously impacted with high extraction voltages.

  2. Microscopic description of the polyamorphic phases of triphenyl phosphite by means of multidimensional solid-state NMR spectroscopy.

    PubMed

    Senker, Jürgen; Sehnert, Jan; Correll, Sascha

    2005-01-12

    The structural properties of a second, apparently amorphous phase (aII) of the molecular glass former triphenyl phosphite were studied by means of multidimensional solid-state NMR spectroscopy and X-ray diffraction. Phase aII was prepared by annealing the supercooled liquid in the temperature range 210 K microscopic description of phase aII. For T(a) > 223 K a nano- or microcrystalline material is formed, whereas for T(a) < 223 K phase aII is homogeneous and disordered. Our data strongly suggest that some of the TPP molecules in phase aII tend toward a parallel alignment. The regions, where the molecules preferentially align appear to be spatially separated and consist of only a few molecules. Whereas the mean cubic expansion of an individual region does not change within the experimental error, the percentage of correlated molecules increases with rising T(a). Based on our results, phase aII can consistently be described as a second liquid, where a part of the molecules exhibit structural correlations. The transformation of the supercooled liquid into phase aII is therefore considered as a liquid-liquid phase transition.

  3. A microscopic frictional theory for reactions in condensed phases: Influence of nonlinear couplings

    NASA Astrophysics Data System (ADS)

    Nagaoka, Masataka; Yoshida, Naoto; Yamabe, Tokio

    1996-10-01

    On the assumption of external bath equilibrium, a set of simultaneous linear generalized Langevin equations (GLE) for a microscopic Hamiltonian is derived, whose potential function includes cubic (i.e., nonlinear) coupling terms, which are linear in internal coordinates but quadratic in external bath coordinates. Furthermore, on the linear GLE treatment, a closed expression of time-dependent friction coefficient and a rate constant in the Grote-Hynes theory (GHT) are derived microscopically, reflecting the reactant and solvent structures. By comparing the rate constant of GHT with that of the multidimensional transition-state theory (TST) for the whole solution system, we conclude that these rate expressions are different from each other and the deviation is due to the dynamic effect via the nonlinear coupling among the reaction, internal, and external normal coordinates. Moreover, the friction coefficient depends on temperature and the deviation becomes larger with temperature increasing. By the second-order perturbation theory, we have estimated the deviation which is approximately equal to a transmission coefficient κ, for a real cluster reaction system: the formic acid-water-water system. We have obtained κ of 0.92, which is smaller than unity. A mode analysis shows that two hindered translational motions of the solvent with low frequencies prevent the reaction from proceeding. Besides, we have investigated the isotope effect of a medium water molecule and found that the dynamic isotope effect for the reaction is quite large, i.e., κ for heavy water is much smaller than that for light water. Not the change of the reactive frequency on the free energy surface but that of the frictional effect in the deuterium substitution mainly contributes to the isotope effect. Further, the temperature dependence of κ for the reaction has been estimated and it is found that κ becomes smaller with temperature increasing and the change of the frictional effect in

  4. Extracting Constitutive Stress-Strain Behavior of Microscopic Phases by Micropillar Compression

    NASA Astrophysics Data System (ADS)

    Williams, J. J.; Walters, J. L.; Wang, M. Y.; Chawla, N.; Rohatgi, A.

    2013-02-01

    The macroscopic behavior of metallic materials is a complex function of microstructure. The size, morphology, volume fraction, crystallography, and distribution of a 2nd phase within a surrounding matrix all control the mechanical properties. Understanding the contributions of the individual microconstituents to the mechanical behavior of multiphase materials has proven difficult due to the inability to obtain accurate constitutive relationships of each individual constituent. In dual-phase steels, for example, the properties of martensite or ferrite in bulk form are not representative of their behavior at the microscale. In this study, micropillar compression was employed to determine the mechanical properties of individual microconstituents in metallic materials with "composite" microstructures, consisting of two distinct microconstituents: (I) a Mg-Al alloy with pure Mg dendrites and eutectic regions and (II) a powder metallurgy steel with ferrite and martensite constituents. The approach is first demonstrated in a Mg-Al directionally solidified alloy where the representative stress-strain behavior of the matrix and eutectic phases was obtained. The work is then extended to a dual-phase steel where the constitutive behavior of the ferrite and martensite were obtained. Here, the results were also incorporated into a modified rule-of-mixtures approach to predict the composite behavior of the steel. The constitutive behavior of the ferrite and martensite phases developed from micropillar compression was coupled with existing strength-porosity models from the literature to predict the ultimate tensile strength of the steel. Direct comparisons of the predictions with tensile tests of the bulk dual-phase steel were conducted and the correlations were quite good.

  5. Imaging performance of attenuated phase-shift mask using coherent scattering microscope

    NASA Astrophysics Data System (ADS)

    Lee, Jae Uk; Jeong, SeeJun; Hong, Seong Chul; Lee, Seung Min; Ahn, Jinho

    2014-03-01

    The half-tone phase shift mask (PSM) has been suggested for better imaging performances like image contrast, NILS and H-V bias compared to the binary mask (BIM) in EUV lithography. In this paper, we measured imaging performance of a fabricated half-tone attenuated PSM with Coherent Scattering Microscopy (CSM) and the results were compared with simulation data obtained by EM-suite tool. We prepared a half-tone attenuated PSM which has 12.7% reflectivity and 180° phase shift with absorber stack of 16.5mn-thick TaN absorber and 24nm-thick Mo phase shifter. With CSM, an actinic inspection tool, we measured the imaging properties of PSM. The diffraction efficiencies of BIM were measured as 31%, 36%, and 44% for 88 nm, 100 nm, and 128 nm mask CD, respectively, while those of PSM were measured as 45%, 62%, and 81%. Also the aerial image at wafer level obtained by CSM with high volume manufacturing tool's (HVM) illumination condition (NA=0.33, σ=0.9) showed higher image contrast and NILS with phase shift effect. And the measured data were consistent with the simulation data.

  6. Neutron Diffraction Residual Strain Tensor Measurements Within The Phase IA Weld Mock-up Plate P-5

    SciTech Connect

    Hubbard, Camden R

    2011-09-01

    Oak Ridge National Laboratory (ORNL) has worked with NRC and EPRI to apply neutron and X-ray diffraction methods to characterize the residual stresses in a number of dissimilar metal weld mockups and samples. The design of the Phase IA specimens aimed to enable stress measurements by several methods and computational modeling of the weld residual stresses. The partial groove in the 304L stainless steel plate was filled with weld beads of Alloy 82. A summary of the weld conditions for each plate is provided in Table 1. The plates were constrained along the long edges during and after welding by bolts with spring-loaded washers attached to the 1-inch thick Al backing plate. The purpose was to avoid stress relief due to bending of the welded stainless steel plate. The neutron diffraction method was one of the methods selected by EPRI for non-destructive through thickness strain and stress measurement. Four different plates (P-3 to P-6) were studied by neutron diffraction strain mapping, representing four different welding conditions. Through thickness neutron diffraction strain mappings at NRSF2 for the four plates and associated strain-free d-zero specimens involved measurement along seven lines across the weld and at six to seven depths. The mountings of each plate for neutron diffraction measurements were such that the diffraction vector was parallel to each of the three primary orthogonal directions of the plate: two in-plane directions, longitudinal and transverse, and the direction normal to the plate (shown in left figure within Table 1). From the three orthogonal strains for each location, the residual stresses along the three plate directions were calculated. The principal axes of the strain and stress tensors, however, need not necessarily align with the plate coordinate system. To explore this, plate P-5 was selected for examination of the possibility that the principal axes of strain are not along the sample coordinate system axes. If adequate data could

  7. Piezoelectric phased array acousto-ultrasonic interrogation of damage in thin plates

    NASA Astrophysics Data System (ADS)

    Purekar, Ashish S.

    Structural Health Monitoring (SHM) and Condition Based Maintenance (CBM) systems can provide substantial benefits for aging aerospace systems as well as newer systems still in the design process. In aging aerospace systems, a retrofitted SHM system would alert users of incipient damage preventing catastrophic failure. For newer systems, incorporating a SHM approach and using CBM techniques can reduce life-cycle costs. Central to such SHM and CBM systems is the ability to detect damage in a structure. Traditional approaches to damage detection in structures involve one of two methods. In the modal dynamics approach, the natural frequencies and modeshapes of a structure shift when damage occurs. The location, type, and amount of damage is determined by the shifts in the modal properties due to damage. Alternately, in an Ultrasonics approach, the structure is scanned with a specialized transducer which induces high frequency vibrations in the structure. Damage in the structure is inferred when these vibrations are altered. In the same vein as Ultrasonics, Acoustic Emission based methods listen for energy release in the structure upon defect growth. All of these techniques have limitations which hinder their usage in a practical system. This thesis attempts to develop a methodology with the benefits of the modal approach as well as the Ultrasonics/Acoustic Emission approach. The methodology is commonly referred to as an Acousto-Ultrasonic technique for damage detection. The structural dynamics of plate structures is described as wavelike in nature where the plate is a medium for wave propagation. For thin plates, bulk wave propagation is described using Lamb wave modes. The two fundamental modes of wave propagation are the in-plane acoustic mode and the transverse bending mode. The interaction of these waves with a discontinuity or damaged region changes the way the waves propagate. Part of the incident wavefront is reflected back while the rest is transmitted through

  8. Surface wave phase velocities from 2-D surface wave tomography studies in the Anatolian plate

    NASA Astrophysics Data System (ADS)

    Arif Kutlu, Yusuf; Erduran, Murat; Çakır, Özcan; Vinnik, Lev; Kosarev, Grigoriy; Oreshin, Sergey

    2014-05-01

    We study the Rayleigh and Love surface wave fundamental mode propagation beneath the Anatolian plate. To examine the inter-station phase velocities a two-station method is used along with the Multiple Filter Technique (MFT) in the Computer Programs in Seismology (Herrmann and Ammon, 2004). The near-station waveform is deconvolved from the far-station waveform removing the propagation effects between the source and the station. This method requires that the near and far stations are aligned with the epicentre on a great circle path. The azimuthal difference of the earthquake to the two-stations and the azimuthal difference between the earthquake and the station are restricted to be smaller than 5o. We selected 3378 teleseismic events (Mw >= 5.7) recorded by 394 broadband local stations with high signal-to-noise ratio within the years 1999-2013. Corrected for the instrument response suitable seismogram pairs are analyzed with the two-station method yielding a collection of phase velocity curves in various period ranges (mainly in the range 25-185 sec). Diffraction from lateral heterogeneities, multipathing, interference of Rayleigh and Love waves can alter the dispersion measurements. In order to obtain quality measurements, we select only smooth portions of the phase velocity curves, remove outliers and average over many measurements. We discard these average phase velocity curves suspected of suffering from phase wrapping errors by comparing them with a reference Earth model (IASP91 by Kennett and Engdahl, 1991). The outlined analysis procedure yields 3035 Rayleigh and 1637 Love individual phase velocity curves. To obtain Rayleigh and Love wave travel times for a given region we performed 2-D tomographic inversion for which the Fast Marching Surface Tomography (FMST) code developed by N. Rawlinson at the Australian National University was utilized. This software package is based on the multistage fast marching method by Rawlinson and Sambridge (2004a, 2004b). The

  9. Decoding the pair correlations and properties of equilibrium microscopic cluster phases

    NASA Astrophysics Data System (ADS)

    Bollinger, Jonathan; Jadrich, Ryan; Truskett, Thomas

    Due to competing interactions acting between particles, dispersed colloidal suspensions can reversibly transition to phases comprising aggregate clusters. Cluster phases have been reported for both 'model' colloidal particles and complex monomers (e.g., proteins); however, many questions remain regarding how to detect and characterize cluster phases given only pair structural correlations (the information most accessible across diverse systems) and how to relate clustering susceptibility and behavior to underlying monomer-monomer interactions. Using molecular simulations and liquid-state theory across a wide survey of conditions, we decode the widely-observed intermediate range order pre-peak in the structure factor by: (1) validating a physically-intuitive rule for detecting clustering based on the pre-peak thermal correlation length; and (2) relating pre-peak position to cluster size and bulk monomer density. We further demonstrate how clustering transitions and resultant properties relate to monomer interactions along coordinates tunable in experiments. These trends are suitable for comparing against clustering systems that can be directly visualized (via, e.g., confocal microscopy), which should aid in assessing the realism of commonly-adopted monomer interaction potentials.

  10. Translational Symmetry and Microscopic Constraints on Symmetry-Enriched Topological Phases: A View from the Surface

    NASA Astrophysics Data System (ADS)

    Cheng, Meng; Zaletel, Michael; Barkeshli, Maissam; Vishwanath, Ashvin; Bonderson, Parsa

    2016-10-01

    The Lieb-Schultz-Mattis theorem and its higher-dimensional generalizations by Oshikawa and Hastings require that translationally invariant 2D spin systems with a half-integer spin per unit cell must either have a continuum of low energy excitations, spontaneously break some symmetries, or exhibit topological order with anyonic excitations. We establish a connection between these constraints and a remarkably similar set of constraints at the surface of a 3D interacting topological insulator. This, combined with recent work on symmetry-enriched topological phases with on-site unitary symmetries, enables us to develop a framework for understanding the structure of symmetry-enriched topological phases with both translational and on-site unitary symmetries, including the effective theory of symmetry defects. This framework places stringent constraints on the possible types of symmetry fractionalization that can occur in 2D systems whose unit cell contains fractional spin, fractional charge, or a projective representation of the symmetry group. As a concrete application, we determine when a topological phase must possess a "spinon" excitation, even in cases when spin rotational invariance is broken down to a discrete subgroup by the crystal structure. We also describe the phenomena of "anyonic spin-orbit coupling," which may arise from the interplay of translational and on-site symmetries. These include the possibility of on-site symmetry defect branch lines carrying topological charge per unit length and lattice dislocations inducing degeneracies protected by on-site symmetry.

  11. Laves phase in alloy 718 fusion zone — microscopic and calorimetric studies

    SciTech Connect

    Manikandan, S.G.K.; Sivakumar, D.; Prasad Rao, K.; Kamaraj, M.

    2015-02-15

    Microstructural characterization of alloy 718 fusion zone welded with both solid solution and age hardenable filler metal has been done. The microsegregation and the aging response were studied by employing three levels of weld cooling rate. Gas Tungsten Arc welding process was used. The fusion zone of solid solution filler metal has been responding to the aging treatment due to the weld process conditions and weld metal chemistry. However the weld metal composition was modified due to the higher molybdenum (Mo) content in solid solution filler metal. The effect of this modification on the phase reaction temperatures was studied and the same was compared with the conventional filler metal. - Graphical abstract: Display Omitted - Highlights: • Interdendritic segregation has been controlled by weld cooling rate. • Laves phase formation has been studied with cooling rate and weld metal chemistry. • Aging response with solid solution filler metal has been demonstrated. • Reduction in Laves phase and alloying element segregation has been confirmed. • Reaction temperatures were found modified because of Mo addition.

  12. 3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM

    PubMed Central

    Chua, Eugene Y.D.; Vogirala, Vinod K.; Inian, Oviya; Wong, Andrew S.W.; Nordenskiöld, Lars; Plitzko, Juergen M.; Danev, Radostin; Sandin, Sara

    2016-01-01

    The Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device that enables contrast enhancement of biological samples. Here we have evaluated the potential of combining phase-plate imaging and single particle analysis to determine the structure of a small protein–DNA complex. To test the method, we made use of a 200 kDa Nucleosome Core Particle (NCP) reconstituted with 601 DNA for which a high-resolution X-ray crystal structure is known. We find that the phase plate provides a significant contrast enhancement that permits individual NCPs and DNA to be clearly identified in amorphous ice. The refined structure from 26,060 particles has an overall resolution of 3.9 Å and the density map exhibits structural features consistent with the estimated resolution, including clear density for amino acid side chains and DNA features such as the phosphate backbone. Our results demonstrate that phase-plate cryo-EM promises to become an important method to determine novel near-atomic resolution structures of small and challenging samples, such as nucleosomes in complex with nucleosome-binding factors. PMID:27563056

  13. Real-time monitoring of the cell physiological and pathological processes using computer-aided phase microscope

    NASA Astrophysics Data System (ADS)

    Vasilenko, Irina A.; Shabalin, Vladimir; Tychinsky, Vladimir P.; Kufal, Georgy E.; Konradov, A.; Slinchenko, O. I.

    1996-01-01

    Living cells investigation and cell response to external factors are of great interest for practical medicine and biology. The main advantages of computer aided phase microscope (CPM) Cytoscan which permits us to observe the cell surface and internal structure consists in superresolution and the possibility of the dynamic processes registering. We attempt to characterize some aspects of the morphofunctional status of human lymphocytes determining the dynamics of the selected points in normal or pathological cells. To evaluate the lymphocyte homeostasis donors and persons of autoimmune diseases were analyzed and the changes of optical and geometrical cell parameters registered by CPM Cytoscan. The dynamic process registration allowed us to perform the real-time quantitative analysis of the living lymphocyte activity in norm and pathology.

  14. Electron microscopic evidence for a tribologically induced phase transformation as the origin of wear in diamond

    SciTech Connect

    Zhang, Xinyi; Schneider, Reinhard; Müller, Erich; Gerthsen, Dagmar; Mee, Manuel; Meier, Sven; Gumbsch, Peter

    2014-02-14

    Tribological testing of a coarse-grained diamond layer, deposited by plasma-enhanced chemical vapor deposition, was performed on a ring-on-ring tribometer with a diamond counterpart. The origin of the wear of diamond and of the low friction coefficient of 0.15 was studied by analyzing the microstructure of worn and unworn regions by transmission and scanning electron microscopy. In the worn regions, the formation of an amorphous carbon layer with a thickness below 100 nm is observed. Electron energy loss spectroscopy of the C-K ionization edge reveals the transition from sp{sup 3}-hybridized C-atoms in crystalline diamond to a high fraction of sp{sup 2}-hybridized C-atoms in the tribo-induced amorphous C-layer within a transition region of less than 5 nm thickness. The mechanically induced phase transformation from diamond to the amorphous phase is found to be highly anisotropic which is clearly seen at a grain boundary, where the thickness of the amorphous layer above the two differently oriented grains abruptly changes.

  15. Kinetics of phase transition in protein solutions on microscopic and mesoscopic length scales

    NASA Astrophysics Data System (ADS)

    Filobelo, Luis F.

    2005-11-01

    Phase transformations in solutions of macromolecules are fundamental for all living things, and of great importance in science and industry. For instance, insulin is biosynthesized in the beta cells of the pancreas and stored in crystalline form, which protects it form cleavage, until it is needed. Certain diseases such as Alzheimer, sickle cell anemia, and eye cataract are produced by the polymerization of protein molecules, which loose their functionality after the phase transition. Additionally, separation operations in manufacturing of pharmaceuticals can be eliminated if the crystals produced have a narrow size distribution. The nucleation and growth of crystals can be adequately controlled only if the mechanisms that govern these processes are well understood. Here we have investigated several facets of the kinetics controlling the behavior of phase transition in protein solutions. We performed experiments to determine the homogenous nucleation rate for lysozyme and insulin crystals and the contribution of heterogeneously nucleated crystals. In the first segment of this work we discuss the existence of a solution-to-crystal spinodal boundary derived from these determinations, and showed that the formation of crystalline nuclei from solution occur in two steps for lysozyme: the formation of quasi-droplets of a disordered intermediate, followed by the nucleation of ordered crystalline embryos within these droplets in which the rate of each step depends on a respective free energy barrier and on the growth rate of its near-critical clusters. We addressed experimentally the relative significance of the free-energy barriers and the kinetic factors for the nucleation of crystals from solution. Using dynamic and static light scattering along with differential refractometry, we also characterized the appearance of dense liquid droplets and the magnitude of the second osmotic virial coefficient B2 for insulin in both aqueous solution and in solution containing 15% (v

  16. Microscopic analysis of the compressibility in the spinel phase of Si3N4

    NASA Astrophysics Data System (ADS)

    Mori-Sánchez, P.; Franco, R.; Martín Pendás, A.; Luaña, V.; Recio, J. M.

    2001-06-01

    Quantum-mechanical static simulations of the high-pressure spinel phase of Si3N4 have been performed to determine the unit cell geometry and the equation of state from 0 to 50 GPa. The application of the Bader theory to the ab initio electron density leads to quantum consistent atomic charges and volumes, the crystalline bonding graph, and the prediction of a highly ionic bonding. The computed thermodynamic properties have been interpreted in terms of the local behaviour of the Si and N quantum subgroups and constitutive unit cell polyhedra. We show that: i) the bulk compressibility can be estimated from the average of the tetrahedral and octahedral compressibilities, and ii) the N response to pressure controls the macroscopic behaviour. Our conclusions can be useful in the search of very hard, N-based materials.

  17. Microscopic analysis of nuclear quantum phase transitions in the N{approx_equal}90 region

    SciTech Connect

    Li, Z. P.; Niksic, T.; Vretenar, D.; Meng, J.; Lalazissis, G. A.; Ring, P.

    2009-05-15

    The analysis of shape transitions in Nd isotopes, based on the framework of relativistic energy-density functionals and restricted to axially symmetric shapes in T. Niksic, D. Vretenar, G. A. Lalazissis, and P. Ring [Phys. Rev. Lett. 99, 092502 (2007)], is extended to the region Z=60,62,64 with N{approx_equal}90 and includes both {beta} and {gamma} deformations. Collective excitation spectra and transition probabilities are calculated starting from a five-dimensional Hamiltonian for quadrupole vibrational and rotational degrees of freedom, with parameters determined by constrained self-consistent relativistic mean-field calculations for triaxial shapes. The results reproduce available data and show that there is an abrupt change of structure at N=90 that can be approximately characterized by the X(5) analytic solution at the critical point of the first-order quantum phase transition between spherical and axially deformed shapes.

  18. Pulmonary vascular response during phases of canine heartworm disease: scanning electron microscopic study.

    PubMed

    Schaub, R G; Rawlings, C A

    1980-07-01

    Pulmonary arteries and veins of 14 dogs in phases of heartworm disease (Dirofilaria immitis infection) were examined by scanning electron microscopy. Two dogs were infected with D immitis microfilaria only, whereas 12 dogs were infected with adult D immitis. Seven of the dogs infected with adult worms were untreated. Two of these 7 dogs had natural infections of unknown duration introduced by mosquito bite, whereas 5 were experimentally infected for 30 days. The remaining five dogs were experimentally infected for 1 year and had worms removed by drug therapy. These five dogs were maintained 12 months after treatment. Arteries and veins from dogs infected with microfilaria had a continuous sheet of endothelial cells. Arterial endothelium from the seven nontreated dogs infected with adult heartworms exhibited swirling patterns, pore formation, and separation of intercellular junctions. Arteries from all dogs had numerous endothelialized villus protrusions; veins had similar, less extensive changes. Arteries and veins from experimentally infected dogs were similar to naturally infected dogs, indicating the infection procedure produced lesions similar to that normally seen in heartworm disease. The extent of vascular lesions was reduced in four of the five treated dogs that had been infected with adult worms. Adult worms, not microfilaria, may produce the vascular lesions seen in heartworm disease. Lesions will regress if worms are removed from the circulation. Lesions may be caused by generation of humoral factors initiated by the presence of adult worms.

  19. Influence of Two-Phase Thermocapillary Flow on Cryogenic Liquid Retention in Microscopic Pores

    NASA Technical Reports Server (NTRS)

    Schmidt, G. R.; Nadarajah, A.; Chung, T. J.; Karr, G. R.

    1994-01-01

    Previous experiments indicate that the bubble point pressure of spacecraft liquid hydrogen acquisition devices is reduced substantially when the ullage is pressurized with heated hydrogen vapor. The objective is to determine whether the two-phase thermocapillary convection arising from thermodynamic non-equilibrium along the porous surfaces of such devices could lead to this observed degradation in retention performance. We also examine why retention capability appears to be unaffected by pressurization with heated helium or direct heating through the porous structure. Computational assessments based on coupled solution of the flowfield and liquid free surface indicate that for highly wetting fluids in small pores, dynamic pressure and vapor recoil dictate surface morphology and drive meniscus deformation. With superheating, the two terms exert the same influence on curvature and promote mechanical equilibrium, but with subcooling, the pressure distribution produces a suction about the pore center-line that degrades retention. This result points to thermocapillary-induced deformation arising from condensation as the cause for retention loss. It also indicates that increasing the level of non-equilibrium by reducing accommodation coefficient restricts deformation and explains why retention failure does not occur with direct screen heating or helium pressurization.

  20. High-throughput 3D tracking of bacteria on a standard phase contrast microscope

    NASA Astrophysics Data System (ADS)

    Taute, K. M.; Gude, S.; Tans, S. J.; Shimizu, T. S.

    2015-11-01

    Bacteria employ diverse motility patterns in traversing complex three-dimensional (3D) natural habitats. 2D microscopy misses crucial features of 3D behaviour, but the applicability of existing 3D tracking techniques is constrained by their performance or ease of use. Here we present a simple, broadly applicable, high-throughput 3D bacterial tracking method for use in standard phase contrast microscopy. Bacteria are localized at micron-scale resolution over a range of 350 × 300 × 200 μm by maximizing image cross-correlations between their observed diffraction patterns and a reference library. We demonstrate the applicability of our technique to a range of bacterial species and exploit its high throughput to expose hidden contributions of bacterial individuality to population-level variability in motile behaviour. The simplicity of this powerful new tool for bacterial motility research renders 3D tracking accessible to a wider community and paves the way for investigations of bacterial motility in complex 3D environments.

  1. Triggering of leukocytes by phase contrast in imaging cytometry with scanning fluorescence microscope (SFM)

    NASA Astrophysics Data System (ADS)

    Bocsi, József; Pierzchalski, Arkadiusz; Marecka, Monika; Malkusch, Wolf; Tárnok, Attila

    2009-02-01

    Slide-based cytometry (SBC) leads to breakthrough in cytometry of cells in tissues, culture and suspension. Carl Zeiss Imaging Solutions' new automated SFM combines imaging with cytometry. A critical step in image analysis is selection of appropriate triggering signal to detect all objects. Without correct target cell definition analysis is hampered. DNA-staining is among the most common triggering signals. However, the majority of DNA-dyes yield massive spillover into other fluorescence channels limiting their application. By microscopy objects of >5μm diameter can be easily detected by phase-contrast signal (PCS) without any staining. Aim was to establish PCS - triggering for cell identification. Axio Imager.Z1 motorized SFM was used (high-resolution digital camera, AxioCam MRm; AxioVision software: automatic multi-channel scanning, analysis). Leukocytes were stained with FITC (CD4, CD8) and APC (CD3) labelled antibodies in combinations using whole blood method. Samples were scanned in three channels (PCS/FITC/APC). Exposition-times for PCS were set as low as possible; the detection efficiency was verified by fluorescence. CD45-stained leukocytes were counted and compared to the number of PCS detected events. Leukocyte subtyping was compared with other cytometers. In focus the PCS of cells showed ring-form that was not optimal for cell definition. Out of focus PCS allows more effective qualitative and quantitative cell analyses. PCS was an accurate triggering signal for leukocytes enabling cell counting and discrimination of leukocytes from platelets. Leukocyte subpopulation frequencies were comparable to those obtained by other cytometers. In conclusion PCS is a suitable trigger-signal not interfering with fluorescence detection.

  2. Testing Single Phase IGBT H-Bridge Switch Plates for the High Voltage Converter Modulator at the Spallation Neutron Source

    SciTech Connect

    Peplov, Vladimir V; Anderson, David E; Solley, Dennis J

    2014-01-01

    Three IGBT H-bridge switching networks are used in each High Voltage Converter Modulator (HVCM) system at the Spallation Neutron Source (SNS) to generate drive currents to three boost transformer primaries switching between positive and negative bus voltages at 20 kHz. Every switch plate assembly is tested before installing it into an operational HVCM. A Single Phase Test Stand has been built for this purpose, and it is used for adjustment, measurement and testing of different configurations of switch plates. This paper will present a description of the Test Stand configuration and discuss the results of testing switch plates with two different types of IGBT gate drivers currently in use on the HVCM systems. Comparison of timing characteristics of the original and new drivers and the resulting performance reinforces the necessity to replace the original H-bridge network drivers with the upgraded units.

  3. 3D tracking and phase-contrast imaging by twin-beams digital holographic microscope in microfluidics

    NASA Astrophysics Data System (ADS)

    Miccio, L.; Memmolo, P.; Finizio, A.; Paturzo, M.; Merola, F.; Grilli, S.; Ferraro, P.

    2012-06-01

    A compact twin-beam interferometer that can be adopted as a flexible diagnostic tool in microfluidic platforms is presented. The devise has two functionalities, as explained in the follow, and can be easily integrated in microfluidic chip. The configuration allows 3D tracking of micro-particles and, at same time, furnishes Quantitative Phase-Contrast maps of tracked micro-objects by interference microscopy. Experimental demonstration of its effectiveness and compatibility with biological field is given on for in vitro cells in microfluidic environment. Nowadays, several microfluidic configuration exist and many of them are commercially available, their development is due to the possibility for manipulating droplets, handling micro and nano-objects, visualize and quantify processes occurring in small volumes and, clearly, for direct applications on lab-on-a chip devices. In microfluidic research field, optical/photonics approaches are the more suitable ones because they have various advantages as to be non-contact, full-field, non-invasive and can be packaged thanks to the development of integrable optics. Moreover, phase contrast approaches, adapted to a lab-on-a-chip configurations, give the possibility to get quantitative information with remarkable lateral and vertical resolution directly in situ without the need to dye and/or kill cells. Furthermore, numerical techniques for tracking of micro-objects needs to be developed for measuring velocity fields, trajectories patterns, motility of cancer cell and so on. Here, we present a compact holographic microscope that can ensure, by the same configuration and simultaneously, accurate 3D tracking and quantitative phase-contrast analysis. The system, simple and solid, is based on twin laser beams coming from a single laser source. Through a easy conceptual design, we show how these two different functionalities can be accomplished by the same optical setup. The working principle, the optical setup and the mathematical

  4. Microscopic identification of Chinese medicinal materials based on X-ray phase contrast imaging: from qualitative to quantitative

    NASA Astrophysics Data System (ADS)

    Xue, Y.; Liang, Z.; Tan, H.; Ni, L.; Zhao, Z.; Xiao, T.; Xu, H.

    2016-07-01

    Although a variety of methods, ranging from simple morphological examination to physical and chemical analysis, and DNA molecular biology, exist for authenticating Chinese medicinal materials(CMMs), no methods can achieve both the source species identification and quality evaluation of CMMs simultaneously. Furthermore, the methods that are currently available for the identification of CMMs, including both optical and electronic microscopy, usually entail strict requirements for sample preparation or testing environment, such as the slicing of super-thin sections, or processing with specific chemical reagents. These treatments not only damage the CMMs but may also cause some of the original microstructures to be missed. Additionally, they may even yield false results. Owing to the unique penetrating character of X-rays, X-ray phase contrast imaging(XPCI) can be used to realize the inner microstructures of CMMs through nondestructive imaging. With the higher flux and luminance of the third generation of synchrotron radiation facility, XPCI can provides clearer and finer microstructures of CMMs, which are mainly composed of C, H, O, and N elements, with better spatial and density resolutions. For more than ten years, the X-ray imaging group at the Shanghai Institute of Applied Physics has investigated the microstructures of CMMs by XPCI and they have established and developed a quantitative X-ray phase contrast micro-CT for investigating the characteristic microstructures of CMMs. During this period, a variety of typical CMMs have been investigated, from two-dimensional (2D) radiography to three-dimensional (3D) micro-CT, from qualitative to quantitative. Taken together, these results verify that quantitative X-ray phase contrast micro-CT is a practical tool for the microscopic investigation of CMMs. Additionally, further efforts are being made to find the relationship between the microstructures' quantitative factors and active chemical components. At present

  5. A simple and cost-effective solid-phase protein nano-assay using polyacrylamide-coated glass plates.

    PubMed

    Krajewski, Wladyslaw A

    2015-02-01

    A new solid-phase protein nano-assay is suggested for simple and sensitive estimation of protein content in sample buffers (a 1-μl sample is sufficient for analysis). The assay is different from conventional "on-filter" assays in that it uses inexpensive fully transparent polyacrylamide gel (PAAG)-coated glass plates as solid support and, thus, combines the convenience of "on-membrane" staining with the sensitivity and ease of documentation of "in-gel" staining (and, therefore, is especially suited for standard lab gel documentation systems). The PAAG plates assay is compatible with all dyes for in-gel protein staining. Depending on the sensitivity of the staining protocol, the assay can be used in macro-, micro-, and nano-assay formats. We also describe a low-cost two-component colloidal Coomassie brilliant blue G-250 (CBB G-250) staining protocol for fast quantitative visualization of proteins spotted on a PAAG plate (the detection limit is up to 2 ng of proteins even when using a Nikon CoolPix digital camera and white light transilluminator instead of a gel scanner). The suggested colloidal CBB G-250 protocol could also be used for visualizing nano-amounts of proteins in polyacrylamide gels. The PAAG plate assay could be useful for proteomic applications and, in general, for all cases where a fast, sensitive, and easily documentable cost-effective solid-phase protein assay is required.

  6. Fluid-structure interaction study of the splitter plate in a TBCC exhaust system during mode transition phase

    NASA Astrophysics Data System (ADS)

    Guo, Shuai; Xu, Jinglei; Mo, Jianwei; Gu, Rui; Pang, Lina

    2015-07-01

    Splitter plate plays an important role in a turbine-based combined-cycle (TBCC) exhaust system during the mode transition phase when turbojet engine and ramjet engine operate simultaneously. Dissimilar pressure distribution on both sides of the plate has a potential origin in the aeroelastic coupling, which is an interesting topic while few research works have devoted to that aspect. To better understand the aeroelastic behavior of the plate and the corresponding dynamic flow features, an integrated fluid-structure interaction simulation is conducted under one particular operation condition during mode transition phase in the TBCC exhaust system. A finite-volume-based CFD solver FLUENT is adopted to solve the unsteady Reynolds average Navier-Stokes equations. ABAQUS, a finite-element-method-based CSD solver, is employed to compute the plate elastic deformation. A two-way interaction between the fluid and the structure is accomplished by the mesh-based parallel-code coupling interface (MpCCI) in a loosely-coupled manner. The accuracy of the coupling procedure is validated for the flutter of a flat plate in supersonic flow. Then, features of steady flow field of the TBCC exhaust system are discussed, followed by the investigation of the aeroelastic phenomenon of the splitter plate and the evolution process of the flow field pattern. Finally, performances variation of the exhaust system is obtained and discussed. The results show that the plate vibrates with decaying amplitude and reaches a dynamic stable state eventually. The thrust, lift and pitch moment of the TBCC exhaust system are increased by 0.68%, 2.82% and 5.86%, respectively, compared with the corresponding values in steady state which does not take into account the fluid-structure interaction effects. The analysis reveals the importance of considering the fluid-structure interaction effects in designing the splitter plate in the TBCC exhaust system and demonstrates the availability of the present coupled

  7. Skeletal growth phases of the cold-water coral Lophelia pertusa shown by scanning electron microscope and electron backscatter diffraction

    NASA Astrophysics Data System (ADS)

    Mouchi, Vincent; Vonlanthen, Pierre; Verrecchia, Eric P.; Crowley, Quentin G.

    2016-04-01

    Lophelia pertusa is a cold-water coral, which may form reefs by the association of multiple coralites within which a polyp lives. Each individual polyp builds an aragonite skeleton by an initial phase of early mineralization (traditionally referred to as centres of calcification) from which aragonite fibres grow in thickening deposits. The skeleton wall features successive optically opaque and translucent bands previously attributed to different regimes of growth as either uniform in crystal orientation (translucent bands) or with a chaotic organization (opaque bands). The processes involved in any organizational changes are still unknown. Microlayers in the coral wall, which represent separate periods of skeletal growth, have been recently identified and described. These growth patterns are readily visible under scanning electron microscope (SEM) after etching in dilute formic acid, but they do not necessarily form continuously visible structures. Here we present high quality SEM images and electron backscatter diffraction (EBSD) maps to study aragonite fibre orientation across the wall of L. pertusa. Both microlayers and opaque and translucent bands are compared to the crystallographic orientation of the aragonite fibres. EBSD maps and SEM images indicate that aragonite fibres do not exhibit a chaotic orientation, even in opaque bands. The absence of continuity of microlayers is partially explained by an association of multiple crystallographic preferred orientations of aragonite fibres. In the case of L. pertusa, careful textural characterisation is necessary prior to elemental or isotope analysis in order to select a skeletal transect representing a linear and continuous time period.

  8. Effect of fringe-artifact correction on sub-tomogram averaging from Zernike phase-plate cryo-TEM

    PubMed Central

    Kishchenko, Gregory P.; Danev, Radostin; Fisher, Rebecca; He, Jie; Hsieh, Chyongere; Marko, Michael; Sui, Haixin

    2015-01-01

    Zernike phase-plate (ZPP) imaging greatly increases contrast in cryo-electron microscopy, however fringe artifacts appear in the images. A computational de-fringing method has been proposed, but it has not been widely employed, perhaps because the importance of de-fringing has not been clearly demonstrated. For testing purposes, we employed Zernike phase-plate imaging in a cryo-electron tomographic study of radial-spoke complexes attached to microtubule doublets. We found that the contrast enhancement by ZPP imaging made nonlinear denoising insensitive to the filtering parameters, such that simple low-frequency band-pass filtering made the same improvement in map quality. We employed sub-tomogram averaging, which compensates for the effect of the “missing wedge” and considerably improves map quality. We found that fringes (caused by the abrupt cut-on of the central hole in the phase plate) can lead to incorrect representation of a structure that is well-known from the literature. The expected structure was restored by amplitude scaling, as proposed in the literature. Our results show that de-fringing is an important part of image-processing for cryo-electron tomography of macromolecular complexes with ZPP imaging. PMID:26210582

  9. Effect of fringe-artifact correction on sub-tomogram averaging from Zernike phase-plate cryo-TEM.

    PubMed

    Kishchenko, Gregory P; Danev, Radostin; Fisher, Rebecca; He, Jie; Hsieh, Chyongere; Marko, Michael; Sui, Haixin

    2015-09-01

    Zernike phase-plate (ZPP) imaging greatly increases contrast in cryo-electron microscopy, however fringe artifacts appear in the images. A computational de-fringing method has been proposed, but it has not been widely employed, perhaps because the importance of de-fringing has not been clearly demonstrated. For testing purposes, we employed Zernike phase-plate imaging in a cryo-electron tomographic study of radial-spoke complexes attached to microtubule doublets. We found that the contrast enhancement by ZPP imaging made nonlinear denoising insensitive to the filtering parameters, such that simple low-frequency band-pass filtering made the same improvement in map quality. We employed sub-tomogram averaging, which compensates for the effect of the "missing wedge" and considerably improves map quality. We found that fringes (caused by the abrupt cut-on of the central hole in the phase plate) can lead to incorrect representation of a structure that is well-known from the literature. The expected structure was restored by amplitude scaling, as proposed in the literature. Our results show that de-fringing is an important part of image-processing for cryo-electron tomography of macromolecular complexes with ZPP imaging.

  10. On-sky Performance Analysis of the Vector Apodizing Phase Plate Coronagraph on MagAO/Clio2

    NASA Astrophysics Data System (ADS)

    Otten, Gilles P. P. L.; Snik, Frans; Kenworthy, Matthew A.; Keller, Christoph U.; Males, Jared R.; Morzinski, Katie M.; Close, Laird M.; Codona, Johanan L.; Hinz, Philip M.; Hornburg, Kathryn J.; Brickson, Leandra L.; Escuti, Michael J.

    2017-01-01

    We report on the performance of a vector apodizing phase plate coronagraph that operates over a wavelength range of 2–5 μm and is installed in MagAO/Clio2 at the 6.5 m Magellan Clay telescope at Las Campanas Observatory, Chile. The coronagraph manipulates the phase in the pupil to produce three beams yielding two coronagraphic point-spread functions (PSFs) and one faint leakage PSF. The phase pattern is imposed through the inherently achromatic geometric phase, enabled by liquid crystal technology and polarization techniques. The coronagraphic optic is manufactured using a direct-write technique for precise control of the liquid crystal pattern and multitwist retarders for achromatization. By integrating a linear phase ramp to the coronagraphic phase pattern, two separated coronagraphic PSFs are created with a single pupil-plane optic, which makes it robust and easy to install in existing telescopes. The two coronagraphic PSFs contain a 180° dark hole on each side of a star, and these complementary copies of the star are used to correct the seeing halo close to the star. To characterize the coronagraph, we collected a data set of a bright (mL = 0–1) nearby star with ∼1.5 hr of observing time. By rotating and optimally scaling one PSF and subtracting it from the other PSF, we see a contrast improvement by 1.46 magnitudes at 3.5 λ /D. With regular angular differential imaging at 3.9 μm, the MagAO vector apodizing phase plate coronagraph delivers a 5σ {{Δ }}{mag} contrast of 8.3 (={10}-3.3) at 2 λ /D and 12.2 (={10}-4.8) at 3.5 λ /D.

  11. Characterization of second-phase plates in a Gd5Ge3 intermetallic compound

    SciTech Connect

    Cao, Qing; Chumbley, Leonard S.

    2013-05-16

    Rare-earth compounds based on the stoichiometry R5(SixGe1-x)4 (R = rare-earth elements) exhibit many unusual features, including possessing R5(SixGe1-x)3 thin plates which always precipitate from the matrix despite efforts to suppress their formation. In an effort to better understand the unique relationship between these two intermetallic alloy systems, the bulk microstructure of the compound Gd5Ge3 was examined using scanning (SEM) and transmission electron microscopy (TEM) and optical microscopy. Surprisingly, SEM examination revealed a series of thin plates present in the Gd5Ge3 matrix similar to what is seen in Gd5Ge4. TEM observation revealed that a role reversal had occurred, with the thin plates possessing the orthorhombic structure and composition of Gd5Ge4. The orientation relationship between Gd5Ge4 thin plates and the Gd5Ge3 matrix was determined to be Graphic the same relationship reported for Gd5Ge3 plates precipitating from a Gd5Ge4 matrix. However, by exchanging the respective roles of the phases as regards matrix vs. precipitate, the total number of precipitation variants seen can be increased from two to six. The persistence with which these two intermetallic systems co-exist is truly unique. However, understanding exactly the kinetic and thermodynamic conditions that lead to their unique relationship is hampered by the high formation temperatures at which the observed reaction occurs.

  12. Planar gas chromatography column on glass plate with nanodispersed silica as the stationary phase

    NASA Astrophysics Data System (ADS)

    Platonov, I. A.; Platonov, V. I.; Pavelyev, V. S.; Agafonov, A. N.

    2016-04-01

    The paper presents the GC column in the plane of the glass plate with the adsorption layer nanodispersed silica. Created gas chromatographic column allows to separate a mixture of five alkanes from pentane to nonane in isothermal (90 ° C) mode less than one minute.

  13. Research of beam conditioning technologies using continuous phase plate, Multi-FM smoothing by spectral dispersion and polarization smoothing

    NASA Astrophysics Data System (ADS)

    Zhang, Rui; Jia, Huaiting; Tian, Xiaocheng; Yuan, Haoyu; Zhu, Na; Su, Jingqin; Hu, Dongxia; Zhu, Qihua; Zheng, Wanguo

    2016-10-01

    In the research of inertial confinement fusion, laser plasma interaction (LPI) is becoming a key problem that affects ignition. Here, multi-frequency modulation (Multi-FM) smoothing by spectral dispersion (SSD), continuous phase plate (CPP) and polarization smoothing (PS) were experimentally studied and implemented on the SG-III laser facility. After using these techniques, the far field distribution of SG-Ⅲ laser facility can be adjusted, controlled and repeated accurately. The output spectrums of the cascade phase modulators used for Multi-FM SSD were stable and the FM-to-AM effect can be restrained. Experiments on SG-III laser facility indicate that when the number of color cycles adopts 1, imposing SSD with 3.3 times diffraction limit (TDL) did not lead to pinhole closure in the spatial filters of preamplifier and main amplifiers with 30-TDL pinhole size. The nonuniformity of the focal spots using Multi-FM SSD, CPP and PS drops to 0.18, comparing to 0.26 with CPP+SSD, 0.57 with CPP+PS and 0.84 with only CPP and wedged lens. Polarization smoothing using flat birefringent plate in the convergent beam of final optics assembly (FOA) was studied. The PS plates were manufactured and equipped on SG-III laser facility for LPI research. Combined beam smoothing and polarization manipulation were also studied to solve the LPI problem. Results indicate that through adjusting dispersion directions of SSD beams in a quad, two dimensional SSD can be obtained. Using polarization control plate (PCP), polarization on the near field and far field can be manipulated, providing new method to solve LPI problem in indirect drive laser fusion.

  14. Vortex characteristics of Fraunhofer diffractions of a plane wave by a spiral phase plate limited by pseudoring polygonal apertures.

    PubMed

    Tang, Huiqin; Wang, Taofen; Zhu, Kaicheng

    2008-08-15

    We introduce a multilevel spiral phase plate (SPP) limited by a pseudoring polygonal aperture (PRPA). Such an SPP has the advantages of easier fabrication and greater suppression of the sidelobes of the diffraction field over that generated with a polygonal aperture (PA). The Fraunhofer diffraction fields generated by an SPP with a PRPA or with a PA have the same topological charge features and a similar diffraction pattern. Numerical evaluations show that the maximum bright annular-intensity difference between the diffraction patterns for the SPP with a PRPA and that of a PA does not exceed 3% under optimal design parameters.

  15. Quantitative X-ray wavefront measurements of Fresnel zone plate and K-B mirrors using phase retrieval.

    PubMed

    Huang, Xiaojing; Wojcik, Michael; Burdet, Nicolas; Peterson, Isaac; Morrison, Graeme R; Vine, David J; Legnini, Daniel; Harder, Ross; Chu, Yong S; Robinson, Ian K

    2012-10-08

    A scanning coherent diffraction imaging method was used to reconstruct the X-ray wavefronts produced by a Fresnel zone plate (FZP) and by Kirkpatrick-Baez (KB) focusing mirrors. The ptychographical measurement was conducted repeatedly by placing a lithographed test sample at different defocused planes. The wavefronts, recovered by phase-retrieval at well-separated planes, show good consistency with numerical propagation results, which provides a self-verification. The validity of the obtained FZP wavefront was further confirmed with theoretical predictions.

  16. Microscopic Derivation of the Ginzburg-Landau Equations for the Periodic Anderson Model in the Coexistence Phase of Superconductivity and Antiferromagnetism

    NASA Astrophysics Data System (ADS)

    Val'kov, V. V.; Zlotnikov, A. O.

    2016-12-01

    On the basis of the periodic Anderson model, the microscopic Ginzburg-Landau equations for heavy-fermion superconductors in the coexistence phase of superconductivity and antiferromagnetism have been derived. The obtained expressions are valid in the vicinity of quantum critical point of heavy-fermion superconductors when the onset temperatures of antiferromagnetism and superconductivity are sufficiently close to each other. It is shown that the formation of antiferromagnetic ordering causes a decrease of the critical temperature of superconducting transition and order parameter in the phase of coexisting superconductivity and antiferromagnetism.

  17. Anisotropic contrast optical microscope.

    PubMed

    Peev, D; Hofmann, T; Kananizadeh, N; Beeram, S; Rodriguez, E; Wimer, S; Rodenhausen, K B; Herzinger, C M; Kasputis, T; Pfaunmiller, E; Nguyen, A; Korlacki, R; Pannier, A; Li, Y; Schubert, E; Hage, D; Schubert, M

    2016-11-01

    An optical microscope is described that reveals contrast in the Mueller matrix images of a thin, transparent, or semi-transparent specimen located within an anisotropic object plane (anisotropic filter). The specimen changes the anisotropy of the filter and thereby produces contrast within the Mueller matrix images. Here we use an anisotropic filter composed of a semi-transparent, nanostructured thin film with sub-wavelength thickness placed within the object plane. The sample is illuminated as in common optical microscopy but the light is modulated in its polarization using combinations of linear polarizers and phase plate (compensator) to control and analyze the state of polarization. Direct generalized ellipsometry data analysis approaches permit extraction of fundamental Mueller matrix object plane images dispensing with the need of Fourier expansion methods. Generalized ellipsometry model approaches are used for quantitative image analyses. These images are obtained from sets of multiple images obtained under various polarizer, analyzer, and compensator settings. Up to 16 independent Mueller matrix images can be obtained, while our current setup is limited to 11 images normalized by the unpolarized intensity. We demonstrate the anisotropic contrast optical microscope by measuring lithographically defined micro-patterned anisotropic filters, and we quantify the adsorption of an organic self-assembled monolayer film onto the anisotropic filter. Comparison with an isotropic glass slide demonstrates the image enhancement obtained by our method over microscopy without the use of an anisotropic filter. In our current instrument, we estimate the limit of detection for organic volumetric mass within the object plane of ≈49 fg within ≈7 × 7 μm(2) object surface area. Compared to a quartz crystal microbalance with dissipation instrumentation, where contemporary limits require a total load of ≈500 pg for detection, the instrumentation demonstrated here improves

  18. Anisotropic contrast optical microscope

    NASA Astrophysics Data System (ADS)

    Peev, D.; Hofmann, T.; Kananizadeh, N.; Beeram, S.; Rodriguez, E.; Wimer, S.; Rodenhausen, K. B.; Herzinger, C. M.; Kasputis, T.; Pfaunmiller, E.; Nguyen, A.; Korlacki, R.; Pannier, A.; Li, Y.; Schubert, E.; Hage, D.; Schubert, M.

    2016-11-01

    An optical microscope is described that reveals contrast in the Mueller matrix images of a thin, transparent, or semi-transparent specimen located within an anisotropic object plane (anisotropic filter). The specimen changes the anisotropy of the filter and thereby produces contrast within the Mueller matrix images. Here we use an anisotropic filter composed of a semi-transparent, nanostructured thin film with sub-wavelength thickness placed within the object plane. The sample is illuminated as in common optical microscopy but the light is modulated in its polarization using combinations of linear polarizers and phase plate (compensator) to control and analyze the state of polarization. Direct generalized ellipsometry data analysis approaches permit extraction of fundamental Mueller matrix object plane images dispensing with the need of Fourier expansion methods. Generalized ellipsometry model approaches are used for quantitative image analyses. These images are obtained from sets of multiple images obtained under various polarizer, analyzer, and compensator settings. Up to 16 independent Mueller matrix images can be obtained, while our current setup is limited to 11 images normalized by the unpolarized intensity. We demonstrate the anisotropic contrast optical microscope by measuring lithographically defined micro-patterned anisotropic filters, and we quantify the adsorption of an organic self-assembled monolayer film onto the anisotropic filter. Comparison with an isotropic glass slide demonstrates the image enhancement obtained by our method over microscopy without the use of an anisotropic filter. In our current instrument, we estimate the limit of detection for organic volumetric mass within the object plane of ≈49 fg within ≈7 × 7 μm2 object surface area. Compared to a quartz crystal microbalance with dissipation instrumentation, where contemporary limits require a total load of ≈500 pg for detection, the instrumentation demonstrated here improves

  19. Numerical analysis on performance of naphthalene phase change thermal storage system in aluminum plate-fin unit

    NASA Astrophysics Data System (ADS)

    Yang, Lin; Peng, Hao; Ling, Xiang; Dong, Huihua

    2015-02-01

    This paper presents a numerical simulation for the prediction of time, temperature and liquid fraction during the melting and solidification process in the latent heat storage of phase changed materials (PCM). The mathematical model, regarding the solid-liquid phase change heat transfer based on the enthalpy formulation, has been proposed. Naphthalene was used as the PCM which is contained in up layer of plate-fin thermal storage. Transient simulations were performed using a commercial computational fluid dynamics package, Fluent, based on the finite volume method. This computational model endeavored to describe both the melting and solidification processes of the PCM. A series of numerical calculations have been done in order to analyses the influence of several heat transfer fluid operating conditions and several fin geometric parameters on the thermal storage unit. Numerical results, which could be used for operating conditions and geometry optimization, provide guideline for the design of the latent thermal energy storage system.

  20. Numerical Simulation of Temperature Controlled Solid Phase Forming Process of Polymeric Plate

    NASA Astrophysics Data System (ADS)

    Liu, Haipeng; Hu, Ping; Fu, Zhengchun; Yan, Yue; Zhang, Guanli

    2005-08-01

    In this paper, a new forming process of polymeric plate called stretch-forming was simulated by adopting the thermo-elasto-visco-plastic large deformation finite element formulation based on Updated Lagrangian method. Some details of this forming process were also studied. It was shown that temperature has important effect on the forming process. Slide contact was assumed in the simulation. Friction force was ignored due to good lubrication. The adopted algorithm is verified by analyzing a thermoforming problem provided in a NUMIFORM'98 paper and comparing simulation results with experimental measures in that paper.

  1. Bleached phase holograms exposed on Agfa-Gevaert 10E75 NAH plates

    NASA Astrophysics Data System (ADS)

    Kumar, S.; Singh, K.

    1991-02-01

    An experimental study has been made of the diffraction efficiency, scattering and stability against printout effect of bleached, photographically recorded two- beam interference gratings using Agfa-Gevaert 10E75 NAH plates. The efficiency is increased by using bleaching processes that convert the silver image into a dielectric image. Plots of the diffraction efficiency and scattering against exposure, and variation of the maximum diffraction efficiency as a function of departure from the Bragg angle, and exposure to white light, for various bleaching processes, are given. A maximum diffraction efficiency of approximately 54% has been achieved by using a potassium iodide and iodine bleach process.

  2. Effect of sodium dodecylbenzene sulfonate on the motion of three-phase contact lines on the Wilhelmy plate surface.

    PubMed

    Karakashev, Stoyan I; Phan, Chi M; Nguyen, Anh V

    2005-11-15

    The combined approach of the molecular-kinetic and hydrodynamic theories for description of the motion of three-phase gas-liquid-solid contact lines has been examined using the Wilhelmy plate method. The whole dynamic meniscus has been divided into molecular, hydrodynamic, and static-like regions. The Young-Laplace equation and the molecular-kinetic and hydrodynamic dewetting theories have been applied to describe the meniscus profiles and contact angle. The dissipative forces accompanying the dynamic dewetting have also been investigated. The experiments with a Wilhelmy plate made from an acrylic polymer sheet were carried out using a computerized apparatus for contact angle analysis (OCA 20, DataPhysics, Germany). The extrapolated dynamic contact angle versus velocity of the three-phase contact line for Milli-Q water and 5x10(-4) M SDBS solution was experimentally obtained and compared with the combined MHD models with low and moderate Reynolds numbers. The models predict similar results for the extrapolated contact angle. SDBS decreases the equilibrium contact angle and increases the molecular jumping length but does not affect the molecular frequency significantly. The hydrodynamic deformation of the meniscus, viscous dissipation, and friction were also influenced by the SDBS surfactant.

  3. Oxidation of ammonium sulfite by a multi-needle-to-plate gas phase pulsed corona discharge reactor

    NASA Astrophysics Data System (ADS)

    Ren, Hua; Lu, Na; Shang, Kefeng; Li, Jie; Wu, Yan

    2013-03-01

    The oxidation of ammonium sulfite in the ammonia-based flue gas desulfurization (FGD) process was investigated in a multi-needle-to-plate gas phase pulsed corona discharge reactor in this paper. The effect of several parameters, including capacitance and peak pulse voltage of discharge system, electrode gap and bubbling gas flow rate on the oxidation rate of ammonium sulfite was reviewed. The oxidation rate of ammonium sulfite could reach 47.2% at the capacitance, the peak pulse voltage, electrode gap and bubbling gas flow rate equal to 2 nF, -24.6 k V, 35 mm and 4 L min-1 within treatment time of 40 min The experimental results indicate that the gas phase pulsed discharge system with a multi-needle-to-plate electrode can oxide the ammonium sulfite. The oxidation rate increased with the applied capacitance and peak pulse voltage and decreased with the electrode gap. As the bubbling gas flow rate increased, the oxidation rate increased first and then tended to reach a stationary value. These results would be important for the process optimization of the (NH4)2SO3 to (NH4)2SO4 oxidation.

  4. Multiview holographic 3D dynamic display by combining a nano-grating patterned phase plate and LCD.

    PubMed

    Wan, Wenqiang; Qiao, Wen; Huang, Wenbin; Zhu, Ming; Ye, Yan; Chen, Xiangyu; Chen, Linsen

    2017-01-23

    Limited by the refreshable data volume of commercial spatial light modulator (SLM), electronic holography can hardly provide satisfactory 3D live video. Here we propose a holography based multiview 3D display by separating the phase information of a lightfield from the amplitude information. In this paper, the phase information was recorded by a 5.5-inch 4-view phase plate with a full coverage of pixelated nano-grating arrays. Because only amplitude information need to be updated, the refreshing data volume in a 3D video display was significantly reduced. A 5.5 inch TFT-LCD with a pixel size of 95 μm was used to modulate the amplitude information of a lightfield at a rate of 20 frames per second. To avoid crosstalk between viewing points, the spatial frequency and orientation of each nano-grating in the phase plate was fine tuned. As a result, the transmission light converged to the viewing points. The angular divergence was measured to be 1.02 degrees (FWHM) by average, slightly larger than the diffraction limit of 0.94 degrees. By refreshing the LCD, a series of animated sequential 3D images were dynamically presented at 4 viewing points. The resolution of each view was 640 × 360. Images for each viewing point were well separated and no ghost images were observed. The resolution of the image and the refreshing rate in the 3D dynamic display can be easily improved by employing another SLM. The recoded 3D videos showed the great potential of the proposed holographic 3D display to be used in mobile electronics.

  5. Microscope basics.

    PubMed

    Sluder, Greenfield; Nordberg, Joshua J

    2013-01-01

    This chapter provides information on how microscopes work and discusses some of the microscope issues to be considered in using a video camera on the microscope. There are two types of microscopes in use today for research in cell biology-the older finite tube-length (typically 160mm mechanical tube length) microscopes and the infinity optics microscopes that are now produced. The objective lens forms a magnified, real image of the specimen at a specific distance from the objective known as the intermediate image plane. All objectives are designed to be used with the specimen at a defined distance from the front lens element of the objective (the working distance) so that the image formed is located at a specific location in the microscope. Infinity optics microscopes differ from the finite tube-length microscopes in that the objectives are designed to project the image of the specimen to infinity and do not, on their own, form a real image of the specimen. Three types of objectives are in common use today-plan achromats, plan apochromats, and plan fluorite lenses. The concept of mounting video cameras on the microscope is also presented in the chapter.

  6. Analytical Round Robin for Elastic-Plastic Analysis of Surface Cracked Plates: Phase I Results

    NASA Technical Reports Server (NTRS)

    Wells, D. N.; Allen, P. A.

    2012-01-01

    An analytical round robin for the elastic-plastic analysis of surface cracks in flat plates was conducted with 15 participants. Experimental results from a surface crack tension test in 2219-T8 aluminum plate provided the basis for the inter-laboratory study (ILS). The study proceeded in a blind fashion given that the analysis methodology was not specified to the participants, and key experimental results were withheld. This approach allowed the ILS to serve as a current measure of the state of the art for elastic-plastic fracture mechanics analysis. The analytical results and the associated methodologies were collected for comparison, and sources of variability were studied and isolated. The results of the study revealed that the J-integral analysis methodology using the domain integral method is robust, providing reliable J-integral values without being overly sensitive to modeling details. General modeling choices such as analysis code, model size (mesh density), crack tip meshing, or boundary conditions, were not found to be sources of significant variability. For analyses controlled only by far-field boundary conditions, the greatest source of variability in the J-integral assessment is introduced through the constitutive model. This variability can be substantially reduced by using crack mouth opening displacements to anchor the assessment. Conclusions provide recommendations for analysis standardization.

  7. [Microscopic colitis].

    PubMed

    Bohr, Johan

    2002-02-11

    Microscopic colitis is an umbrella term for a newly described group of colitides, belonging to the inflammatory bowel diseases, which are only diagnosable by microscopic evaluation of a macroscopically normal colon mucosa. Collagenous colitis and lymphocytic colitis are the most common of these colitides. Microscopic colitis is characterised clinically by chronic non-bloody watery diarrhoea. Crampy abdominal pain, nocturnal diarrhoea, urgency, and initial weight loss are usual. Concomitant diseases of autoimmune origin and arthralgia are commonly seen. Treatment of microscopic colitis follows the guidelines for treatment of other inflammatory bowel diseases, but a substantial part of the patients with microscopic colitis enter spontaneous remission after some years. A minor part, however, have very troublesome symptoms and are almost refractory to treatment. Microscopic colitis has apparently no malignant potential.

  8. Wind loads on flat plate photovoltaic array fields. Phase III, final report

    SciTech Connect

    Miller, R.D.; Zimmerman, D.K.

    1981-04-01

    The results of an experimental analysis (boundary layer wind tunnel test) of the aerodynamic forces resulting from winds acting on flat plate photovoltaic arrays are presented. Local pressure coefficient distributions and normal force coefficients on the arrays are shown and compared to theoretical results. Parameters that were varied when determining the aerodynamic forces included tilt angle, array separation, ground clearance, protective wind barriers, and the effect of the wind velocity profile. Recommended design wind forces and pressures are presented, which envelop the test results for winds perpendicular to the array's longitudinal axis. This wind direction produces the maximum wind loads on the arrays except at the array edge where oblique winds produce larger edge pressure loads.

  9. Plate Tectonics Constrained by Evidence-Based Magmatic Temperatures and Phase Relations of Fertile Lherzolite (Invited)

    NASA Astrophysics Data System (ADS)

    Green, D. H.; Falloon, T.

    2010-12-01

    In order to understand Earth’s plate tectonics we must interpret the most direct probes for mantle composition and temperature distribution i.e. the primitive basaltic magmas and peridotites representing partial melts and mantle residues. An evidence-based approach to identification of parental magmas and determination of their temperatures requires glass and phenocryst compositions and experimentally calibrated Fe/Mg partitioning between olivine and melt. We have compared magmatic crystallization temperatures between ‘hot-spot’(proposed to be plume-related) and normal mid-ocean ridge basalt (MORB) parental liquids, by examining three representative magmatic suites from both ocean island (Hawaii, Iceland, and Réunion) and mid-ocean ridge settings (Cocos-Nazca, East Pacific Rise, and Mid-Atlantic Ridge). We have glass and olivine phenocryst compositions, including volatile (H2O) contents, and have calculated parental liquid compositions at 0.2GPa by incrementally adding olivine back into the glass compositions until a liquid in equilibrium with the most-magnesian olivine phenocryst composition is obtained. The results of these calculations demonstrate that there is very little difference (maximum of ~20°C) between the ranges of crystallization temperatures of the parental liquids (MORB:1243-1351°C versus OIB:1286-1372°C) when volatile contents are taken into account. However while lacking temperature contrast, the source regions for ‘hot-spot’ parental magmas contain geochemical signatures of old subducted crust/lithosphere. The mantle depths of origin determined for both the MORB and OIB suites are similar (MORB:1-2 GPa; OIB:1-2.5 GPa). Calculations of mantle potential temperatures (Tp) are model dependent, particularly to melt fraction from an inferred source. Assuming similar fertile lherzolite sources, the differences in Tp values between the hottest MORB and the hottest ocean island tholeiite sources are ~80°C. These differences disappear if the

  10. Phase transformations during the Ag-In plating and bonding of vertical diode elements of multijunction solar cells

    SciTech Connect

    Klochko, N. P. Khrypunov, G. S.; Volkova, N. D.; Kopach, V. R.; Lyubov, V. N.; Kirichenko, M. V.; Momotenko, A. V.; Kharchenko, N. M.; Nikitin, V. A.

    2013-06-15

    The conditions of the bonding of silicon multijunction solar cells with vertical p-n junctions using Ag-In solder are studied. The compositions of electrodeposited indium films on silicon wafers silver plated by screen printing and silver and indium films fabricated by layer-by-layer electrochemical deposition onto the surface of silicon vertical diode cells silver plated in vacuum are studied. Studying the electrochemical-deposition conditions, structure, and surface morphology of the grown layers showed that guaranteed bonding is provided by 8-min heat treatment at 400 Degree-Sign C under the pressure of a stack of metallized silicon wafers; however, the ratio of the indium and silver layer thicknesses should not exceed 1: 3. As this condition is satisfied, the solder after wafer bonding has the InAg{sub 3} structure (or InAg{sub 3} with an Ag phase admixture), due to which the junction melting point exceeds 700 Degree-Sign C, which guarantees the functioning of such solar cells under concentrated illumination.

  11. TLC of alkaloids on cyanopropyl bonded stationary phases. Part II. Connection with RP18 and silica plates.

    PubMed

    Petruczynik, Anna; Waksmundzka-Hajnos, Monika; Plech, Tomasz; Tuzimski, Tomasz; Hajnos, Michał Ł; Jóźwiak, Grzegorz; Gadzikowska, Maria; Rompała, Anna

    2008-04-01

    Some standards of the alkaloids and synthetic or natural mixtures are separated by two-dimensional thin-layer chromatography (TLC) on different adsorbent layers. Normal- and reversed-phase systems are used to obtain significant differences in the separation selectivity. Optimization of the one-dimensional TLC separation of the alkaloids' standards is performed on cyanopropyl-silica, RP18W, and silica layers in various eluents containing (besides diluent and modifier) silanol blockers, such as diethyl amine or ammonia. The most selective systems are used for the separation of the alkaloids' mixtures by two-dimensional TLC with an adsorbent gradient method. The mixtures of alkaloids or plant extracts (Chelidonium majus, Fumaria officinalis, or Glaucium flavum) are chromatographed in system I; the plates are connected with the plate pre-coated with various adsorbent, and partly separated fractions are transferred to the second layer and developed in system II. CN-silica-RP18W and CN-silica-silica are used as the connected layers. The alkaloids are identified by R(F) values of standards, and the components of plant extracts are identified in both systems, and by the comparison of UV spectra obtained in diode array detector densitometry.

  12. Hard X-ray Microscopic Imaging Of Human Breast Tissues

    NASA Astrophysics Data System (ADS)

    Park, Sung H.; Kim, Hong T.; Kim, Jong K.; Jheon, Sang H.; Youn, Hwa S.

    2007-01-01

    X-ray microscopy with synchrotron radiation will be a useful tool for innovation of x-ray imaging in clinical and laboratory settings. It helps us observe detailed internal structure of material samples non-invasively in air. And, it also has the potential to solve some tough problems of conventional breast imaging if it could evaluate various conditions of breast tissue effectively. A new hard x-ray microscope with a spatial resolution better than 100 nm was installed at Pohang Light Source, a third generation synchrotron radiation facility in Pohang, Korea. The x-ray energy was set at 6.95 keV, and the x-ray beam was monochromatized by W/B4C monochromator. Condenser and objective zone plates were used as x-ray lenses. Zernike phase plate next to condenser zone plate was introduced for improved contrast imaging. The image of a sample was magnified 30 times by objective zone plate and 20 times by microscope objective, respectively. After additional 10 times digital magnification, the total magnifying power was up to 6000 times in the end. Phase contrast synchrotron images of 10-μm-thick female breast tissue of the normal, fibroadenoma, fibrocystic change and carcinoma cases were obtained. By phase contrast imaging, hard x-rays enable us to observe many structures of breast tissue without sample preparations such as staining or fixation.

  13. Thickness measurement of transparent glass plates using a lateral shearing cyclic path optical configuration setup and polarization phase shifting interferometry.

    PubMed

    Kumar, Y Pavan; Chatterjee, Sanjib

    2010-11-20

    We present a measurement technique to determine the thickness of a transparent glass plate (GP) by using a lateral shearing cyclic path optical configuration (CPOC) setup and polarization phase shifting interferometry (PPSI). In the technique, the GP introduces a longitudinal shift in the focus of the beam and, as a result, a spherical wavefront emerges from the lens, which is otherwise set for producing a collimated beam. Using CPOC, two laterally sheared orthogonally polarized beams are generated from the incident spherical wavefront. By applying PPSI, the slope of the optical path difference variation between the laterally sheared interfering beams is evaluated, and the radius of the spherical wavefront and the longitudinal shift of the beam focus are calculated. The thickness of the GP is determined from the standard relation between the longitudinal shift of the focus introduced by the GP and the thickness of the GP. Results obtained for a GP of 9.810mm thickness are presented.

  14. Measurement of Acoustic Intensity Distribution and Radiation Power of Flat-Plate Phased-Array Sound Source

    NASA Astrophysics Data System (ADS)

    Yokoyama, Tomoki; Takahashi, Kumiko; Seki, Daizaburou; Hasegawa, Akio

    2002-05-01

    The acoustic intensity distribution and radiation power of a flat-plate phased-array sound source consisting of Tonpilz-type transducers were measured. This study shows that the active acoustic intensity is skewed in the direction of wave propagation. In addition, it clarifies that if the measurement is carried out in the immediate vicinity of the sound source, the reactive acoustic intensity distribution is effective for identifying the positions of the individual sound source elements. Experimental values of active radiation power agree well with theoretical values. Conversely, experimental values of reactive radiation power do not agree with theoretical values; it is clear that they fluctuate significantly with distance from the radiating surface. The reason for this is explained in the case of a point sound source.

  15. CMS Resistive Plate Chamber overview, from the present system to the upgrade phase I

    NASA Astrophysics Data System (ADS)

    Paolucci, P.; Hadjiiska, R.; Litov, L.; Pavlov, B.; Petkov, P.; Dimitrov, A.; Beernaert, K.; Cimmino, A.; Costantini, S.; Guillaume, G.; Lellouch, J.; Marinov, A.; Ocampo, A.; Strobbe, N.; Thyssen, F.; Tytgat, M.; Verwilligen, P.; Yazgan, E.; Zaganidis, N.; Aleksandrov, A.; Genchev, V.; Iaydjiev, P.; Rodozov, M.; Shopova, M.; Sultanov, G.; Ban, Y.; Cai, J.; Xue, Z.; Ge, Y.; Li, Q.; Qian, S.; Avila, C.; Chaparro, L. F.; Gomez, J. P.; Gomez Moreno, B.; Osorio Oliveros, A. F.; Sanabria, J. C.; Assran, Y.; Sharma, A.; Abbrescia, M.; Colaleo, A.; Pugliese, G.; Loddo, F.; Calabria, C.; Maggi, M.; Benussi, L.; Bianco, S.; Colafranceschi, S.; Piccolo, D.; Carrillo, C.; Iorio, O.; Buontempo, S.; Vitulo, P.; Berzano, U.; Gabusi, M.; Kang, M.; Lee, K. S.; Park, S. K.; Shin, S.; Kim, M. S.; Seo, H. K.; Goh, J.; Choi, Y.-I.

    2013-04-01

    Resistive Plate Chambers have been chosen as dedicated trigger muon detector for the Compact Muon Solenoid experiment [1] at the Large Hadron Collider [2] at CERN. The system consists of about 3000 m2 of double gap RPC chambers placed in both the barrel and endcap muon regions. About 5.6 fb-1 (2010-2011) of proton-proton collision data have been used to study the performance of the RPC detector and trigger. A full high voltage scan of all the RPC chambers has been done at beginning of 2011 data taking to evaluate the working point chamber by chamber and to eventually spot aging effects. The excellent behaviour of the RPC detector can be summarized with an average detector efficiency of about 97%, an average cluster size of 1.8 and an intrinsic noise rate of 0.1 Hz/cm2. This is a clear fulfilment of all the requirements decided 18 years ago in the CMS TDR document [3].

  16. Using a short-pulse diffraction-limited laser beam to probe filamentation of a random phase plate smoothed beam.

    PubMed

    Kline, J L; Montgomery, D S; Flippo, K A; Johnson, R P; Rose, H A; Shimada, T; Williams, E A

    2008-10-01

    A short pulse (few picoseconds) laser probe provides high temporal resolution measurements to elucidate details of fast dynamic phenomena not observable with typical longer laser pulse probes and gated diagnostics. Such a short pulse laser probe (SPLP) has been used to measure filamentation of a random phase plate (RPP) smoothed laser beam in a gas-jet plasma. The plasma index of refraction due to driven density and temperature fluctuations by the RPP beam perturbs the phase front of a SPLP propagating at a 90 degree angle with respect to the RPP interaction beam. The density and temperature fluctuations are quasistatic on the time scale of the SPLP (approximately 2 ps). The transmitted near-field intensity distribution from the SPLP provides a measure of the phase front perturbation. At low plasma densities, the transmitted intensity pattern is asymmetric with striations across the entire probe beam in the direction of the RPP smoothed beam. As the plasma density increases, the striations break up into smaller sizes along the direction of the RPP beam propagation. The breakup of the intensity pattern is consistent with self-focusing of the RPP smoothed interaction beam. Simulations of the experiment using the wave propagation code, PF3D, are in qualitative agreement demonstrating that the asymmetric striations can be attributed to the RPP driven density fluctuations. Quantification of the beam breakup measured by the transmitted SPLP could lead to a new method for measuring self-focusing of lasers in underdense plasmas.

  17. Engineering of apodizer filters in the optical imaging using a set of phase plates

    NASA Astrophysics Data System (ADS)

    Palillero-Sandoval, Omar; Márquez-Aguilar, Pedro Antonio; Berriel-Valdos, Luis Raul

    2016-10-01

    A numerical study of the performance of a set of phase masks and apodizer filters, which are able to extend the depth of field (DOF) in the imaging system, are presented using a test object with different levels of gray. The ambiguity function is used to display which of these filters, placed in the pupil function, can increase the DOF. The intensity quasi-point spread functions (IQPSFs) show invariance in the central spot of the IQPSF when the apodizer is placed in the optical system. This approach minimizes the replicas of the object that appears in the background of the restored images produced by the numerical processing when in the pupil function these phase filters are used simultaneously. The correlation coefficient criteria and minimum squares differences were used to evaluate the quality of the restoration process.

  18. Relocating Seismicity on the Arctic Plate Boundary Using Teleseismic and Regional Phases and a Bayesian Multiple Event Locator

    NASA Astrophysics Data System (ADS)

    Gibbons, Steven J.; Dahl-Jensen, Trine; Kværna, Tormod; Larsen, Tine B.; Paulsen, Berit; Voss, Peter

    2016-04-01

    The tectonophysics of plate boundaries are illuminated by the pattern of seismicity - and the ability to locate seismic events accurately depends upon the number and quality of observations, the distribution of recording stations, and how well the traveltimes of seismic phases are modelled. The boundary between the Eurasian and North American plates between 70 and 84 degrees North hosts large seismic events which are well recorded teleseismically and many more events at far lower magnitudes that are well recorded only at regional distances. Existing seismic bulletins have considerable spread and bias resulting from limited station coverage and deficiencies in the velocity models applied; this is particularly acute for the lower magnitude events which may only be constrained by a small number of Pn and Sn arrivals. Over the past 15 years, there has been a significant improvement in the seismic network in the Arctic - a difficult region to instrument due to the harsh climate, a sparsity of quiet and accessible sites, and the expense and difficult logistics of deploying and maintaining stations. New deployments and upgrades to stations on Greenland, Svalbard, and the islands Jan Mayen, Hopen, and Bjørnøya have resulted in a sparse but stable regional seismic network which results in events down to magnitudes below 3 generating high quality Pn and Sn signals on multiple stations. A catalog of over 1000 events in the region since 1998 has been generated using many new phase readings on stations on both sides of the spreading ridge in addition to teleseismic P phases. The Bayesloc program, a Bayesian hierarchical multiple event location algorithm, has been used to relocate the full set of events iteratively and this has resulted in a significant reduction in the spread in hypocenter estimates for both large and small events. Whereas single event location algorithms minimize the vector of time residuals on an event-by-event basis, Bayesloc favours the hypocenters which

  19. Microscopic dynamics of AC{sub 60} compounds in the plastic, polymer, and dimer phases investigated by inelastic neutron scattering

    SciTech Connect

    Schober, H.; Toelle, A.; Renker, B.; Heid, R.; Gompf, F.

    1997-09-01

    We present inelastic neutron-scattering results for AC{sub 60} (A=K,Rb,Cs) compounds. The spectra of the high-temperature fcc phases strongly resemble the ones of pristine C{sub 60} in the plastic phase. At equal temperatures we find identical rotational diffusion constants for pristine C{sub 60} and Rb{sub 1}C{sub 60} (D{sub r}=2.4 10{sup 10} s{sup {minus}1} at 400 K). The changes taking place in the inelastic part of the spectra on cooling AC{sub 60} indicate the formation of strong intermolecular bonds. The buildup of intensities in the gap region separating internal and external vibrations in pure C{sub 60} is the most prominent signature of this transition. The spectra of the low-temperature phases depend on their thermal history. The differences can be explained by the formation of a polymer phase (upon slow cooling from the fcc phase) and a dimer phase (upon fast cooling), respectively. The experimental data are analyzed on the basis of lattice dynamical calculations. The density-of-states are well modeled assuming a [2+2] bond for the polymer and a single intercage bond for the dimer. Indications for different intercage bonding are also found in the internal mode spectra, which, on the other hand, react only weakly to the charge transfer. The dimer phase is metastable and converts into the polymer phase with a strongly temperature-dependent time constant. The transition from the polymer to the fcc phase is accompanied by inelastic precursor effects which are interpreted as the signature of inhomogeneities arising from plastic monomer regions embedded in the polymer phase. In the polymer phase AC{sub 60} compounds show strong anharmonic behavior in the low-temperature region. The possible connection with the metal-to-insulator transition is discussed. {copyright} {ital 1997} {ital The American Physical Society}

  20. Origin of phase shift in atomic force microscopic investigation of the surface morphology of NR/NBR blend film.

    PubMed

    Thanawan, S; Radabutra, S; Thamasirianunt, P; Amornsakchai, T; Suchiva, K

    2009-01-01

    Atomic force microscopy (AFM) was used to study the morphology and surface properties of NR/NBR blend. Blends at 1/3, 1/1 and 3/1 weight ratios were prepared in benzene and formed film by casting. AFM phase images of these blends in tapping mode displayed islands in the sea morphology or matrix-dispersed structures. For blend 1/3, NR formed dispersed phase while in blends 1/1 and 3/1 phase inversion was observed. NR showed higher phase shift angle in AFM phase imaging for all blends. This circumstance was governed by adhesion energy hysteresis between the device tip and the rubber surface rather than surface stiffness of the materials, as proved by force distance measurements in the AFM contact mode.

  1. Direct observation of electronic-liquid-crystal phase transitions and their microscopic origin in La1/3Ca2/3MnO3

    PubMed Central

    Tao, J.; Sun, K.; Yin, W.-G.; Wu, L.; Xin, H.; Wen, J. G.; Luo, W.; Pennycook, S. J.; Tranquada, J. M.; Zhu, Y.

    2016-01-01

    The ground-state electronic order in doped manganites is frequently associated with a lattice modulation, contributing to their many interesting properties. However, measuring the thermal evolution of the lattice superstructure with reciprocal-space probes alone can lead to ambiguous results with competing interpretations. Here we provide direct observations of the evolution of the superstructure in La1/3Ca2/3MnO3 in real space, as well as reciprocal space, using transmission electron microscopic (TEM) techniques. We show that the transitions are the consequence of a proliferation of dislocations plus electronic phase separation. The resulting states are well described by the symmetries associated with electronic-liquid-crystal (ELC) phases. Moreover, our results resolve the long-standing controversy over the origin of the incommensurate superstructure and suggest a new structural model that is consistent with recent theoretical calculations. PMID:27874069

  2. Direct observation of electronic-liquid-crystal phase transitions and their microscopic origin in La1/3Ca2/3MnO3

    DOE PAGES

    Tao, J.; Sun, K.; Yin, W. -G.; ...

    2016-11-22

    The ground-state electronic order in doped manganites is frequently associated with a lattice modulation, contributing to their many interesting properties. However, measuring the thermal evolution of the lattice superstructure with reciprocal-space probes alone can lead to ambiguous results with competing interpretations. Here, we provide direct observations of the evolution of the superstructure in La1/3Ca2/3MnO3 in real space, as well as reciprocal space, using transmission electron microscopic (TEM) techniques. We show that the transitions are the consequence of a proliferation of dislocations plus electronic phase separation. The resulting states are well described by the symmetries associated with electronic-liquid-crystal (ELC) phases. Furthermore,more » our results resolve the long-standing controversy over the origin of the incommensurate superstructure and suggest a new structural model that is consistent with recent theoretical calculations.« less

  3. Direct observation of electronic-liquid-crystal phase transitions and their microscopic origin in La1/3Ca2/3MnO3

    NASA Astrophysics Data System (ADS)

    Tao, J.; Sun, K.; Yin, W.-G.; Wu, L.; Xin, H.; Wen, J. G.; Luo, W.; Pennycook, S. J.; Tranquada, J. M.; Zhu, Y.

    2016-11-01

    The ground-state electronic order in doped manganites is frequently associated with a lattice modulation, contributing to their many interesting properties. However, measuring the thermal evolution of the lattice superstructure with reciprocal-space probes alone can lead to ambiguous results with competing interpretations. Here we provide direct observations of the evolution of the superstructure in La1/3Ca2/3MnO3 in real space, as well as reciprocal space, using transmission electron microscopic (TEM) techniques. We show that the transitions are the consequence of a proliferation of dislocations plus electronic phase separation. The resulting states are well described by the symmetries associated with electronic-liquid-crystal (ELC) phases. Moreover, our results resolve the long-standing controversy over the origin of the incommensurate superstructure and suggest a new structural model that is consistent with recent theoretical calculations.

  4. Direct observation of electronic-liquid-crystal phase transitions and their microscopic origin in La1/3Ca2/3MnO3.

    PubMed

    Tao, J; Sun, K; Yin, W-G; Wu, L; Xin, H; Wen, J G; Luo, W; Pennycook, S J; Tranquada, J M; Zhu, Y

    2016-11-22

    The ground-state electronic order in doped manganites is frequently associated with a lattice modulation, contributing to their many interesting properties. However, measuring the thermal evolution of the lattice superstructure with reciprocal-space probes alone can lead to ambiguous results with competing interpretations. Here we provide direct observations of the evolution of the superstructure in La1/3Ca2/3MnO3 in real space, as well as reciprocal space, using transmission electron microscopic (TEM) techniques. We show that the transitions are the consequence of a proliferation of dislocations plus electronic phase separation. The resulting states are well described by the symmetries associated with electronic-liquid-crystal (ELC) phases. Moreover, our results resolve the long-standing controversy over the origin of the incommensurate superstructure and suggest a new structural model that is consistent with recent theoretical calculations.

  5. Shearing interference microscope for step-height measurements.

    PubMed

    Trịnh, Hưng-Xuân; Lin, Shyh-Tsong; Chen, Liang-Chia; Yeh, Sheng-Lih; Chen, Chin-Sheng; Hoang, Hong-Hai

    2017-03-07

    A shearing interference microscope using a Savart prism as the shear plate is proposed for inspecting step-heights. Where the light beam propagates through the Savart prism and microscopic system to illuminate the sample, it then turns back to re-pass through the Savart prism and microscopic system to generate a shearing interference pattern on the camera. Two measurement modes, phase-shifting and phase-scanning, can be utilized to determine the depths of the step-heights on the sample. The first mode, which employs a narrowband source, is based on the five-step phase-shifting algorithm and has a measurement range of a quarter-wavelength. The second mode, which adopts a broadband source, is based on peak-intensity identification technology and has a measurement range up to a few micrometres. This paper is to introduce the configuration and measurement theory of this microscope, perform a setup used to implement it, and present the experimental results from the uses of the setup. The results not only verify the validity but also confirm the high measurement repeatability of the proposed microscope.

  6. Tensile Residual Stress Mitigation Using Low Temperature Phase Transformation Filler Wire in Welded Armor Plates

    SciTech Connect

    Feng, Zhili; Bunn, Jeffrey R; Tzelepis, Demetrios A; Payzant, E Andrew; Yu, Xinghua

    2016-01-01

    Hydrogen induced cracking (HIC) has been a persistent issue in welding of high-strength steels. Mitigating residual stresses is one of the most efficient ways to control HIC. The current study develops a proactive in-process weld residual stress mitigation technique, which manipulates the thermal expansion and contraction sequence in the weldments during welding process. When the steel weld is cooled after welding, martensitic transformation will occur at a temperature below 400 C. Volume expansion in the weld due to the martensitic transformation will reduce tensile stresses in the weld and heat affected zone and in some cases produce compressive residual stresses in the weld. Based on this concept, a customized filler wire which undergoes a martensitic phase transformation during cooling was developed. The new filler wire shows significant improvement in terms of reducing the tendency of HIC in high strength steels. Bulk residual stress mapping using neutron diffraction revealed reduced tensile and compressive residual stresses in the welds made by the new filler wire.

  7. Two-dimensional X-ray focusing by off-axis grazing incidence phase Fresnel zone plate on the laboratory X-ray source

    NASA Astrophysics Data System (ADS)

    Grigoriev, Maxim; Fakhrtdinov, Rashid; Irzhak, Dmitry; Firsov, Alexander; Firsov, Anatoly; Svintsov, Alexander; Erko, Alexey; Roshchupkin, Dmitry

    2017-02-01

    The results of studying a two-dimensional X-ray focusing by an off-axis grazing incidence phase Fresnel zone plate on the laboratory X-ray source are presented. This optics enables obtaining a focal spot of 2 μm on the laboratory X-ray source with a focusing efficiency of 30% at a high signal/noise ratio.

  8. Microscopic origin of resistance drift in the amorphous state of the phase-change compound GeTe

    NASA Astrophysics Data System (ADS)

    Gabardi, S.; Caravati, S.; Sosso, G. C.; Behler, J.; Bernasconi, M.

    2015-08-01

    Aging is a common feature of the glassy state. In the case of phase-change chalcogenide alloys the aging of the amorphous state is responsible for an increase of the electrical resistance with time. This phenomenon called drift is detrimental in the application of these materials in phase-change nonvolatile memories, which are emerging as promising candidates for storage class memories. By means of combined molecular dynamics and electronic structure calculations based on density functional theory, we have unraveled the atomistic origin of the resistance drift in the prototypical phase-change compound GeTe. The drift results from a widening of the band gap and a reduction of Urbach tails due to structural relaxations leading to the removal of chains of Ge-Ge homopolar bonds. The same structural features are actually responsible for the high mobility above the glass transition which boosts the crystallization speed exploited in the device.

  9. Relative permeability and the microscopic distribution of wetting and nonwetting phases in the pore space of Berea sandstone

    SciTech Connect

    Schlueter, E.M.; Cook, N.G.W. |; Witherspoon, P.A.; Myer, L.R.

    1994-04-01

    Experiments to study relative permeabilities of a partially saturated rock have been carried out in Berea sandstone using fluids that can be solidified in place. The effective permeability of the spaces not occupied by the wetting fluid (paraffin wax) or the nonwetting fluid (Wood`s metal), have been measured at various saturations after solidifying each of the phases. The tests were conducted on Berea sandstone samples that had an absolute permeability of about 600 md. The shape of the laboratory-derived relative permeability vs. saturation curves measured with the other phase solidified conforms well with typical curves obtained using conventional experimental methods. The corresponding wetting and nonwetting fluid distributions at different saturations are presented and analyzed in light of the role of the pore structure in the invasion process, and their impact on relative permeability and capillary pressure. Irreducible wetting and nonwetting phase fluid distributions are studied. The effect of clay minerals on permeability is also assessed.

  10. Desorption atmospheric pressure photoionization with polydimethylsiloxane as extraction phase and sample plate material.

    PubMed

    Vaikkinen, A; Kotiaho, T; Kostiainen, R; Kauppila, T J

    2010-12-03

    Desorption atmospheric pressure photoionization (DAPPI) is an ambient ionization technique for mass spectrometry (MS) that can be used to ionize polar as well as neutral and completely non-polar analytes. In this study polydimethylsiloxane (PDMS) was used as a solid phase extraction sorbent for DAPPI-MS analysis. Pieces of PDMS polymer were soaked in an aqueous sample, where the analytes were sorbed from the sample solution to PDMS. After this, the extracted analytes were desorbed directly from the polymer by the hot DAPPI spray solvent plume, without an elution step. Swelling and extracting the PDMS with a cleaning solvent prior to extraction diminished the high background in the DAPPI mass spectrum caused by PDMS oligomers. Acetone, hexane, pentane, toluene, diisopropylamine and triethylamine were tested for this purpose. The amines were most efficient in reducing the PDMS background, but they also suppressed the signals of low proton affinity analytes. Toluene was chosen as the optimum cleaning solvent, since it reduced the PDMS background efficiently and gave intensive signals of most of the studied analytes. The effects of DAPPI spray solvents toluene, acetone and anisole on the PDMS background and the ionization of analytes were also compared and extraction conditions were optimized. Anisole gave a low background for native PDMS, but toluene ionized the widest range of analytes. Analysis of verapamil, testosterone and anthracene from purified, spiked wastewater was performed to demonstrate that the method is suited for in-situ analysis of water streams. In addition, urine spiked with several analytes was analyzed by the PDMS method and compared to the conventional DAPPI procedure, where sample droplets are applied on PMMA surface. With the PDMS method the background ion signals caused by the urine matrix were lower, the S/N ratios of analytes were 2-10 times higher, and testosterone, anthracene and benzo[a]pyrene that were not detected from PMMA in urine

  11. Prediction of CO/sub 2/ flood performance: Interaction of phase behavior with microscopic pore structure heterogeneity

    SciTech Connect

    Dui, K.K.; Orr, F.M.

    1984-09-01

    This paper examines the effects of microscopic heterogeneity, present in rock pore structures or resulting from high water saturations, on the performance of one-dimensional CO/sub 2/ floods. A one-dimensional simulator, shown previously to model slim tube displacements quantitatively, was modified to include effects of an isolated or trapped fraction, unavailable for mixing with injected fluids, and a dendritic fraction, which exchanges material with the flowing fraction by mass transfer. Model formulation, numerical solution and validation tests are described. Results of simulations with no water present indicate that performance of a one-dimensional CO/sub 2/ flood is sensitive to restrictions to local mixing. Calculated oil recovery decreases as the flowing fraction, Peclet number and Damkohler number decrease. Comparisons of calculated and measured residual oil saturations in CO/sub 2/ core floods support this observation. Comparison of secondary and tertiary displacements by continuous CO/sub 2/ injection and alternate and simultaneous injection of CO/sub 2/ and water indicates that performance of secondary displacements is not strongly affected by restricted local mixing. In tertiary displacements, however, total oil recovery and the rate of recovery are reduced if effects of trapped and dendritic saturations are included.

  12. Microscopic Polyangiitis

    PubMed Central

    Chung, Sharon A.; Seo, Philip

    2010-01-01

    Synopsis In 1923, Friedrich Wohlwill described two patients with a “microscopic form of periarteritis nodosa”, which was distinct from classical polyarteritis nodosa. This disease, now known as microscopic polyangiitis (MPA), is a primary systemic vasculitis characterized by inflammation of the small-caliber blood vessels and the presence of circulating antineutrophil cytoplasmic antibodies (ANCA). Typically, microscopic polyangiitis presents with glomerulonephritis and pulmonary capillaritis, although involvement of the skin, nerves, and gastrointestinal tract is not uncommon. Treatment of MPA generally requires use of a cytotoxic agent (such as cyclophosphamide) in addition to high-dose glucocorticoids. Recent research has focused on identifying alternate treatment strategies that minimize or eliminate exposure to cytotoxic agents. This article will review the history, pathogenesis, clinical manifestations, and treatment of MPA. PMID:20688249

  13. μCT-Based Analysis of the Solid Phase in Foams: Cell Wall Corrugation and other Microscopic Features.

    PubMed

    Pardo-Alonso, Samuel; Solórzano, Eusebio; Vicente, Jerome; Brabant, Loes; Dierick, Manuel L; Manke, Ingo; Hilger, Andr; Laguna, Ester; Rodriguez-Perez, Miguel Angel

    2015-10-01

    This work presents a series of three-dimensional computational methods with the objective of analyzing and quantifying some important structural characteristics in a collection of low-density polyolefin-based foams. First, the solid phase tortuosity, local thickness, and surface curvature, have been determined over the solid phase of the foam. These parameters were used to quantify the presence of wrinkles located at the cell walls of the foams under study. In addition, a novel segmentation technique has been applied to the continuous solid phase. This novel method allows performing a separate analysis of the constituting elements of this phase, that is, cell struts and cell walls. The methodology is based on a solid classification algorithm and evaluates the local topological dissimilarities existing between these elements. Thanks to this method it was possible to perform a separate analysis of curvature, local thickness, and corrugation ratio in the solid constituents that reveals additional differences that were not detected in the first analysis of the continuous structure. The methods developed in this work are applicable to other types of porous materials in fields such as geoscience or biomedicine.

  14. Molecular orientation behavior of chiral nematic liquid crystals based on the presence of blue phases using polarized microscopic FT-IR spectroscopy

    NASA Astrophysics Data System (ADS)

    Matsumura, Masanori; Katayama, Norihisa

    2016-07-01

    Study on molecular orientation behavior of highly twisted chiral nematic liquid crystals (N∗LCs) expressing blue phases (BPs) is important for developing new devices. This study examines the change of molecular orientation of N∗LCs due to the presence of BPs. Polarized microscopic FT-IR spectroscopy was used to study the in- and out-of-plane molecular orientations of N∗LCs that undergo a phase transition involving BPs. The band intensity ratio of CN to CH2 stretching modes (CN/CH2) in the IR spectra was used to determine the orientation of N∗LC molecules. The measured spectra indicated that the helical axis of N∗LC molecules was perpendicular to the substrate before heating and inclined on the substrate after cooling the sample which has phase transition from BP I to chiral nematic (N∗). The N∗LC molecule in the cell of rubbed orientation film exhibited the in-plane anisotropy after a heating-cooling ramp only in samples that passed through BP I. These results indicate that the changes of molecular orientation of N∗LC by phase transition are affected by BP I.

  15. Martian Microscope

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The microscopic imager (circular device in center) is in clear view above the surface at Meridiani Planum, Mars, in this approximate true-color image taken by the panoramic camera on the Mars Exploration Rover Opportunity. The image was taken on the 9th sol of the rover's journey. The microscopic imager is located on the rover's instrument deployment device, or arm. The arrow is pointing to the lens of the instrument. Note the dust cover, which flips out to the left of the lens, is open. This approximated color image was created using the camera's violet and infrared filters as blue and red.

  16. Near Fault Observatories (NFO) services and integration plan for European Plate Observing System (EPOS) Implementation Phase

    NASA Astrophysics Data System (ADS)

    Chiaraluce, Lauro

    2016-04-01

    the services provided by other Thematic Core Services for the standard data (e.g. seismic and geodetic) and on the direct access to the e-infrastructures of individual NFOs via the Integrated Core Services web services for access and distribution of non standard data (e.g. strain- and tilt-meters, geochemical and electro- magneto-telluric data). We will collaborate with the other groups possessing the same data on data harmonization in terms of both format and metadata description to optimise and facilitate the integration and interoperability processes. The services will include a Virtual Laboratory, novel visualization tools for data and products describing the anatomy of active faults and the physical processes governing earthquake generation. VL is an online engagement and knowledge sharing initiative for communicating to the other scientists, stockholders and the public the state of scientific knowledge concerning earthquake source and tectonic processes generating catastrophic events. The availability of real-time data provides the unique opportunity of observing all phases of the earthquake rupture. It is thus of crucial importance developing methodologies to follow in real-time the evolution of the event (e.g. Earthquake Early Warning systems). NFOs are ideal infrastructures for hosting testing centers where a variety of scientific algorithms for real-time monitoring can be independently evaluated. Besides the interest for fundamental science, such developments have a societal impact and can attract new stakeholders such as industry partners who are interested in adopting in such (e.g. EEW) technologies.

  17. Phase diagrams and microscopic structures of (Hg,Cd)Te, (Hg,Zn)Te, and (Cd,Zn)Te alloys

    NASA Technical Reports Server (NTRS)

    Patrick, R. S.; Chen, A.-B.; Sher, A.; Berding, M. A.

    1988-01-01

    A cluster theory based on the quasi-chemical approximation has been applied to study the local correlation bond-length distribution, and phase diagrams of the II-VI pseudobinary alloys Hg(1 - x)Cd(x)Te, Hg(1 - x)Zn(x)Te, and Cd(1 - x)Zn(x)Te. The cluster energy is calculated by letting it relax in some effective alloy medium and then considering the contributions from the strain and chemical energies. Two different models are presented to simulate the alloy medium. While both models show that all three alloys have nearly random distributions, the signs of the local correlation prove to be sensitive to the alloy medium chosen for the energy calculation. Good agreement is found between experiment and the bond lengths and phase diagrams in both models.

  18. Microscopic colitis

    PubMed Central

    Ianiro, Gianluca; Cammarota, Giovanni; Valerio, Luca; Annicchiarico, Brigida Eleonora; Milani, Alessandro; Siciliano, Massimo; Gasbarrini, Antonio

    2012-01-01

    Microscopic colitis may be defined as a clinical syndrome, of unknown etiology, consisting of chronic watery diarrhea, with no alterations in the large bowel at the endoscopic and radiologic evaluation. Therefore, a definitive diagnosis is only possible by histological analysis. The epidemiological impact of this disease has become increasingly clear in the last years, with most data coming from Western countries. Microscopic colitis includes two histological subtypes [collagenous colitis (CC) and lymphocytic colitis (LC)] with no differences in clinical presentation and management. Collagenous colitis is characterized by a thickening of the subepithelial collagen layer that is absent in LC. The main feature of LC is an increase of the density of intra-epithelial lymphocytes in the surface epithelium. A number of pathogenetic theories have been proposed over the years, involving the role of luminal agents, autoimmunity, eosinophils, genetics (human leukocyte antigen), biliary acids, infections, alterations of pericryptal fibroblasts, and drug intake; drugs like ticlopidine, carbamazepine or ranitidine are especially associated with the development of LC, while CC is more frequently linked to cimetidine, non-steroidal antiinflammatory drugs and lansoprazole. Microscopic colitis typically presents as chronic or intermittent watery diarrhea, that may be accompanied by symptoms such as abdominal pain, weight loss and incontinence. Recent evidence has added new pharmacological options for the treatment of microscopic colitis: the role of steroidal therapy, especially oral budesonide, has gained relevance, as well as immunosuppressive agents such as azathioprine and 6-mercaptopurine. The use of anti-tumor necrosis factor-α agents, infliximab and adalimumab, constitutes a new, interesting tool for the treatment of microscopic colitis, but larger, adequately designed studies are needed to confirm existing data. PMID:23180940

  19. Search for microscopic and macroscopic biaxiality in the cybotactic nematic phase of new oxadiazole bent-core mesogens

    NASA Astrophysics Data System (ADS)

    Kim, Young-Ki; Cukrov, Greta; Vita, Francesco; Scharrer, Eric; Samulski, Edward T.; Francescangeli, Oriano; Lavrentovich, Oleg D.

    2016-06-01

    The possibility of biaxial orientational order in nematic liquid crystals is a subject of intense current interest. We explore the tendencies toward local and global biaxial ordering in the recently synthesized trimethylated oxadiazole-based bent-core mesogens with a pronounced asymmetric (bow-type) shape of molecules. The combination of x-ray diffraction and optical studies suggests that the biaxial order is expressed differently at the short- and long-range scales. Locally, at the scale of a few molecules, x-ray-diffraction data demonstrate biaxial packing. However, above the mesoscopic scale, the global orientational order in all three compounds is uniaxial, as evidenced by uniform homeotropic alignment of the nematic phase which is optically tested over the entire temperature range and by the observations of topological defects induced by individual and aggregated colloidal spheres in the nematic bulk.

  20. Acoustic imaging microscope

    DOEpatents

    Deason, Vance A.; Telschow, Kenneth L.

    2006-10-17

    An imaging system includes: an object wavefront source and an optical microscope objective all positioned to direct an object wavefront onto an area of a vibrating subject surface encompassed by a field of view of the microscope objective, and to direct a modulated object wavefront reflected from the encompassed surface area through a photorefractive material; and a reference wavefront source and at least one phase modulator all positioned to direct a reference wavefront through the phase modulator and to direct a modulated reference wavefront from the phase modulator through the photorefractive material to interfere with the modulated object wavefront. The photorefractive material has a composition and a position such that interference of the modulated object wavefront and modulated reference wavefront occurs within the photorefractive material, providing a full-field, real-time image signal of the encompassed surface area.

  1. Atomic Force Microscope Mediated Chromatography

    NASA Technical Reports Server (NTRS)

    Anderson, Mark S.

    2013-01-01

    The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a

  2. High-resolution quasi-monochromatic X-ray imaging using a Fresnel phase zone plate and a multilayer mirror.

    PubMed

    Do, A; Troussel, Ph; Baton, S D; Dervieux, V; Gontier, D; Lecherbourg, L; Loupias, B; Obst, L; Pérez, F; Renaudin, P; Reverdin, Ch; Rubbelynck, C; Stemmler, Ph; Soullié, G

    2017-01-01

    High-resolution, high-sensitivity X-ray imaging is a real challenge in laser plasma diagnostic to attain reliable data in high-energy density plasma experiments. In this context, ultra-high-intensity lasers generate hot and dense plasma but only in a small volume. An experiment has been performed at the LULI2000 laser facility to diagnose such plasma conditions from thermal spectroscopic data. To image the emission zone plasma's Al Heβ, a Fresnel-lens-based X-ray imager has been developed. It features a 846 μm-diameter Fresnel Phase Zone Plate (FPZP) and a Pd/B4C multilayer mirror (thickness d = 5.1 nm). This association can be used between 1500 eV and 2100 eV. The FPZP's efficiency was measured on a synchrotron facility (SOLEIL) and its spatial resolution in a laser facility (EQUINOX). The mirror reflectivity was measured on the synchrotron facility BESSY II. With experimental conditions, the system resolution reaches 3.8 ± 0.6 μm with an adequate efficiency in the 1800 eV-1900 eV energy range with a solid angle of 9 × 10(-6) sr. Consequently, a FPZP is an excellent optics setup for high-resolution quasi-monochromatic X-ray imaging and provides a good collection angle. Bragg-Fresnel lenses, based on the principle of FPZP and mirrors, are currently designed for an X-ray imager at the Laser MégaJoule facility.

  3. Real-time monitoring of the functional status of platelets treated by Infukoll using a computer-aided laser phase microscope

    NASA Astrophysics Data System (ADS)

    Vasilenko, Irina A.; Babakova, Svetlana; Kiseleva, Elena; Dyugeev, Adyan; Konradov, Alexander A.; Shabalin, Vladimir

    1999-02-01

    Combined analysis of optic-geometrical characteristics makes it possible to comprehensively evaluate the morphological and functional state of the cytological object, which can not be done during visual observation. The technique is discussed for real-time monitoring of the functional status of platelets using computer-aided phase microscope (CPM) 'Cytoscan'. High accuracy and sensitivity of CPM with respect to determination of local temporal phase make it possible to register the dynamic processes in the voluntarily chosen points and sections of micro-objects, to obtain the Fourier's spectra and other characteristics suitable for statistical analysis. Human platelets were prepared from venous blood of healthy donors and pregnant women by standard methods, suspended in culture medium 199 and treated by different doses of 6% Infukoll HES. Nonfixating and nonstaining cells were studied with CPM: height accuracy 0.5 nm, magnification 1000, acquisition time 4 - 30 s. In our experiments we used time resolution about 0.03 s and 30 x lens with numerical aperture 0.65. During investigations of temporal processes a certain section was chosen in the topogram of cell image and local values for the phase of scattered wave in each of the points of the chosen cell's profile were measured. On the basis of the results of automated phase image analysis of optic-geometrical characteristics of living cells, the new quantitative express-method for evaluating of the functional status of human platelets was developed and tested. The structural changes of cells were visualized in alteration of 3-D images, phase profiles, in the decrease of mean cell phase diameters, heights, volumes, in disturbance of histograms of phase heights distribution by cell image points. New data on the behavior of platelets treated by Infukoll in vitro and in vivo were obtained. Analysis of intracellular dynamics was allowed to characterize the cell's regions of maximal activity, but the intensity of processes

  4. Phase decomposition of γ-U (bcc) in U-10 wt% Mo fuel alloy during hot isostatic pressing of monolithic fuel plate

    NASA Astrophysics Data System (ADS)

    Park, Y.; Eriksson, N.; Newell, R.; Keiser, D. D.; Sohn, Y. H.

    2016-11-01

    Eutectoid decomposition of γ-phase (cI2) into α-phase (oC4) and γ‧-phase (tI6) during the hot isostatic pressing (HIP) of the U-10 wt% Mo (U10Mo) alloy was investigated using monolithic fuel plate samples consisting of U10Mo fuel alloy, Zr diffusion barrier and AA6061 cladding. The decomposition of the γ-phase was observed because the HIP process is carried out near the eutectoid temperature, 555 °C. Initially, a cellular structure, consisting of γ‧-phase surrounded by α-phase, developed from the destabilization of the γ-phase. The cellular structure further developed into an alternating lamellar structure of α- and γ‧-phases. Using scanning electron microscopy and transmission electron microscopy, qualitative and quantitative microstructural analyses were carried out to identify the phase constituents, and elucidate the microstructural development based on time-temperature-transformation diagram of the U10Mo alloy. The destabilization of γ -phase into α- and γ‧-phases would be minimized when HIP process was carried out with rapid ramping/cooling rate and dwell temperature higher than 560 °C.

  5. Single-phase driven ultrasonic motor using two orthogonal bending modes of sandwiching piezo-ceramic plates.

    PubMed

    Ma, Yuting; Choi, Minkyu; Uchino, Kenji

    2016-11-01

    A compact ultrasonic motor using sandwiching piezo-ceramic plates was developed, having advantages of low manufacturing costs, simple driving circuit, and high scalability. The stator is composed of two piezoelectric plates attached to a T-shaped steel body. Two orthogonal bending modes can be excited by driving one piezoelectric plate and the reversed motion of the rotor can be obtained by driving the piezoelectric plate on the opposite side. The prototype stator with a size of 15 mm × 2.44 mm × 2 mm, operated at 44.8 kHz, was experimentally characterized, and a maximum torque of 2 mN m was obtained. Maximum power of 2.3 mW and efficiency of 9% were produced with a load of 0.8 mN m at a rotation speed of 27 rpm.

  6. Single-phase driven ultrasonic motor using two orthogonal bending modes of sandwiching piezo-ceramic plates

    NASA Astrophysics Data System (ADS)

    Ma, Yuting; Choi, Minkyu; Uchino, Kenji

    2016-11-01

    A compact ultrasonic motor using sandwiching piezo-ceramic plates was developed, having advantages of low manufacturing costs, simple driving circuit, and high scalability. The stator is composed of two piezoelectric plates attached to a T-shaped steel body. Two orthogonal bending modes can be excited by driving one piezoelectric plate and the reversed motion of the rotor can be obtained by driving the piezoelectric plate on the opposite side. The prototype stator with a size of 15 mm × 2.44 mm × 2 mm, operated at 44.8 kHz, was experimentally characterized, and a maximum torque of 2 mN m was obtained. Maximum power of 2.3 mW and efficiency of 9% were produced with a load of 0.8 mN m at a rotation speed of 27 rpm.

  7. Electrosorption-enhanced solid-phase microextraction of trace anions using a platinum plate coated with single-walled carbon nanotubes.

    PubMed

    Li, Quanlong; Ding, Yujing; Yuan, Dongxing

    2011-08-15

    A platinum plate coated with single-walled carbon nanotubes (SWCNTs@Pt) was prepared by means of electrophoretic deposition. Using the SWCNTs@Pt plate, an electrosorption-enhanced solid-phase microextraction (EE-SPME) technique was proposed for the extraction of trace anions in water, described as follows: a positive potential was applied to the SWCNTs@Pt plate to extract F(-), Cl(-), Br(-), NO(3)(-) and SO(4)(2-) from water using electrosorption, and then a negative potential was applied to the plate placed in ultra-pure water for the desorption of the absorbed anions, and finally the desorbed anions were analyzed using ion chromatography (IC). The EE-SPME parameters, including extraction potential and time as well as desorption potential and time, were investigated. An analytical method based on the above procedures, i.e., EE-SPME-IC, was established and used for the analysis of trace anions in water. The results showed that the application of potential on the SWCNTs@Pt plate significantly enhanced the ion extraction efficiency, and an enrichment factor of 15-38 was achieved. The SWCNTs@Pt plate could be used more than 50 times without significant decay. The linear range, the limit of detection (S/N=3), the limit of quantification (S/N=10) and repeatability (n=7) of our EE-SPME-IC method were 1.0-150.0 μg/L, 0.06-0.26 μg/L, 0.19-0.85 μg/L and 2.1-8.0%, respectively. The proposed method was successfully applied for the analysis of trace anions in deionized water, and acceptable recoveries between 65.3 and 121.1% were obtained for the spiked deionized water samples.

  8. Differential interference contrast microscopy using Savart plates

    NASA Astrophysics Data System (ADS)

    Trịnh, Hưng-Xuân; Lin, Shyh-Tsong; Chen, Liang-Chia; Yeh, Sheng-Lih; Chen, Chin-Sheng

    2017-04-01

    A new differential interference contrast microscopy (DICM), which uses Savart prisms as a shearing plate and a phase-shifting device, is proposed. The system consists of a phase-shifting module (PSM) and a DICM module (DICMM). The PSM has two Savart prisms: the first prism separates the incident beam into two parallel beams, and the second prism recombines these two beams. The optical path difference (OPD) of the two beams, which is represented by a biased OPD, can be adjusted by rotating the angle of the normal surface of the second prism. In the DICMM, the other Savart prism is used to replace the Nomarski prism (NP) in conventional DICM. It combines with an afocal microscopic system (AMS) to produce a Savart-DICM system, which is able to perform a phase-shifting technique by changing the biased OPD to produce a phase shift of π/2 for each step. This paper describes the configuration and measurement theory of the microscope. The experimental results confirm the validity and capability of the proposed microscope.

  9. Plate-mantle interaction through time explains two-phase uplift history of the eastern Australian passive margin

    NASA Astrophysics Data System (ADS)

    Dietmar Müller, R.; Flament, Nicolas; Matthews, Kara J.; Williams, Simon E.; Gurnis, Michael

    2015-04-01

    The origin of passive margin mountains is a hotly debated topic in geodynamics. The Eastern Highlands of Australia are a type example whose uplift history has been investigated for several decades, with suggested mechanisms ranging from flexural rift shoulder uplift, volcanism and underplating to mantle-convection driven dynamic topography. Most of the highlands have experienced a distinct two-phase uplift history, with the first phase being Late Cretaceous in age, followed by a mid-late Cenozoic renewal in uplift, but the timing and magnitude of uplift differs along strike. We investigate the origin of the Eastern Highlands with a coupled plate-mantle model, using a thorough parameter space analysis, including two alternative subduction boundary evolution models. The first model includes a large (~1000 km width at its maximum extent) Early Cretaceous (140-120 Ma) back-arc basin east of the Lord Howe Rise, representing the now subducted South Loyalty Basin which may have formed due to eastward rollback of the long-lived west-dipping eastern Gondwanaland subduction zone; the alternative scenario is based on the premise that west-dipping subduction is continuous to the East of the Lord Howe Rise between 140-85 Ma, without a large back-arc basin, and the South Loyalty Basin opening as a back arc basin from 85-55 Ma, which is subsequently consumed by subduction. We further investigate the influence of a low-viscosity asthenosphere and of the viscosity profile of the lower mantle on dynamic topography, as well as the effect of changing the buoyancy of the basal dense layer (LLSVP) that contributes to the long-wavelength Pacific superswell. Our best-fit model produces a total uplift up to ~400 m in the interval between 120 and 90-70 Ma, well-matched with recent published estimates from river profile inversion for the Snowy Mountains, New England and the Central Highlands. The driving mechanism is rebound from the eastwards motion of Australia over a sinking slab, first

  10. Microscopic analysis of the superconducting quantum critical point: Finite-temperature crossovers in transport near a pair-breaking quantum phase transition

    NASA Astrophysics Data System (ADS)

    Shah, Nayana; Lopatin, Andrei

    2007-09-01

    A microscopic analysis of the superconducting quantum critical point realized via a pair-breaking quantum phase transition is presented. Finite-temperature crossovers are derived for the electrical conductivity, which is a key probe of superconducting fluctuations. By using the diagrammatic formalism for disordered systems, we are able to incorporate the interplay between fluctuating Cooper pairs and electrons, that is outside the scope of a time-dependent Ginzburg-Landau or effective bosonic action formalism. It is essential to go beyond the standard approximation in order to capture the zero-temperature correction which results purely from the (dynamic) quantum fluctuations and dictates the behavior of the conductivity in an entire low-temperature quantum regime. All dynamic contributions are of the same order and conspire to add up to a negative total, thereby inhibiting the conductivity as a result of superconducting fluctuations. On the contrary, the classical and the intermediate regimes are dominated by the positive bosonic channel. Our theory is applicable in one, two, and three dimensions and is relevant for experiments on superconducting nanowires, doubly connected cylinders, thin films, and bulk in the presence of magnetic impurities, magnetic field, or other pair breakers. A window of nonmonotonic behavior is predicted to exist as either the temperature or the pair-breaking parameter is swept.

  11. Deformation characteristics of {delta} phase in the delta-processed Inconel 718 alloy

    SciTech Connect

    Zhang, H.Y.; Zhang, S.H.; Cheng, M.; Li, Z.X.

    2010-01-15

    The hot working characteristics of {delta} phase in the delta-processed Inconel 718 alloy during isothermal compression deformation at temperature of 950 deg. C and strain rate of 0.005 s{sup -1}, were studied by using optical microscope, scanning electron microscope and quantitative X-ray diffraction technique. The results showed that the dissolution of plate-like {delta} phase and the precipitation of spherical {delta} phase particles coexisted during the deformation, and the content of {delta} phase decreased from 7.05 wt.% to 5.14 wt.%. As a result of deformation breakage and dissolution breakage, the plate-like {delta} phase was spheroidized and transferred to spherical {delta} phase particles. In the center with largest strain, the plate-like {delta} phase disappeared and spherical {delta} phase appeared in the interior of grains and grain boundaries.

  12. Activation of carbon dioxide by a terminal uranium-nitrogen bond in the gas-phase: a demonstration of the principle of microscopic reversibility.

    PubMed

    Dau, Phuong D; Armentrout, P B; Michelini, Maria C; Gibson, John K

    2016-03-14

    Activation of CO2 is demonstrated by its spontaneous dissociative reaction with the gas-phase anion complex NUOCl2(-), which can be considered as NUO(+) coordinated by two chloride anion ligands. This reaction was previously predicted by density functional theory to occur exothermically, without barriers above the reactant energy. The present results demonstrate the validity of the prediction of microscopic reversibility, and provide a rare case of spontaneous dissociative addition of CO2 to a gas-phase complex. The activation of CO2 by NUOCl2(-) proceeds by conversion of a U[triple bond, length as m-dash]N bond to a U[double bond, length as m-dash]O bond and creation of an isocyanate ligand to yield the complex UO2(NCO)Cl2(-), in which uranyl, UO2(2+), is coordinated by one isocyanate and two chloride anion ligands. This activation of CO2 by a uranium(vi) nitride complex is distinctive from previous reports of oxidative insertion of CO2 into lower oxidation state U(iii) or U(iv) solid complexes, during which both C-O bonds remain intact. This unusual observation of spontaneous addition and activation of CO2 by NUOCl2(-) is a result of the high oxophilicity of uranium. If the computed Gibbs free energy of the reaction pathway, rather than the energy, is considered, there are barriers above the reactant asymptotes such that the observed reaction should not proceed under thermal conditions. This result provides a demonstration that energy rather than Gibbs free energy determines reactivity under low-pressure bimolecular conditions.

  13. Formation of III–V ternary solid solutions on GaAs and GaSb plates via solid-phase substitution reactions

    SciTech Connect

    Vasil’ev, V. I.; Gagis, G. S. Kuchinskii, V. I.; Danil’chenko, V. G.

    2015-07-15

    Processes are considered in which ultrathin layers of III–V ternary solid solutions are formed via the delivery of Group-V element vapors to GaAs and GaSb semiconductor plates, with solid-phase substitution reactions occurring in the surface layers of these plates. This method can form defect-free GaAs{sup 1–x}P{sup x}, GaAs{sup x}Sb{sup 1–x}, and GaP{sup x}Sb{sup 1–x} layers with thicknesses of 10–20 nm and a content x of the embedded components of up to 0.04.

  14. Plate motion

    SciTech Connect

    Gordon, R.G. )

    1991-01-01

    The motion of tectonic plates on the earth is characterized in a critical review of U.S. research from the period 1987-1990. Topics addressed include the NUVEL-1 global model of current plate motions, diffuse plate boundaries and the oceanic lithosphere, the relation between plate motions and distributed deformations, accelerations and the steadiness of plate motions, the distribution of current Pacific-North America motion across western North America and its margin, plate reconstructions and their uncertainties, hotspots, and plate dynamics. A comprehensive bibliography is provided. 126 refs.

  15. Densitometric HPTLC method for qualitative, quantitative analysis and stability study of Coenzyme Q10 in pharmaceutical formulations utilizing normal and reversed-phase silica gel plates.

    PubMed

    Abdel-Kader, Maged Saad; Alam, Prawez; Alqasoumi, Saleh Ibrahim

    2016-03-01

    Two simple, precise and stability-indicating densitometric HPTLC method were developed and validated for qualitative and quantitative analysis of Coenzyme Q10 in pharmaceutical formulations using normal-phase (Method I) and reversed phase (Method II) silica gel TLC plates. Both methods were developed and validated with 10×20 cm glass-backed plates coated with 0.2 mm layers of either silica gel 60 F254 (E-Merck, Germany) using hexane-ethyl acetate (8.5:1.5 v/v) as developing system (Method I) or RP-18 silica gel 60 F254 (E-Merck, Germany) using methanol-acetone (4:6 v/v) as mobile phase (Method II). Both analyses were scanned with a densitometer at 282 nm. Linearity was found in the ranges 50-800 ng/spot (r(2)=0.9989) and 50-800 ng/spot (r(2)=0.9987) for Method I and Method II respectively. Stability of Coenzyme Q10 was explored by the two methods using acid, base, hydrogen peroxide, temperature and different solvents. Due to the efficiency of the method in separating Coenzyme Q10 from other ingredients including its degradation products, it can be applied for quality control, standardization of different pharmaceutical formulations and stability study.

  16. On thermodynamic and microscopic reversibility

    SciTech Connect

    Crooks, Gavin E.

    2011-07-12

    The word 'reversible' has two (apparently) distinct applications in statistical thermodynamics. A thermodynamically reversible process indicates an experimental protocol for which the entropy change is zero, whereas the principle of microscopic reversibility asserts that the probability of any trajectory of a system through phase space equals that of the time reversed trajectory. However, these two terms are actually synonymous: a thermodynamically reversible process is microscopically reversible, and vice versa.

  17. Ultra-thin optical vortex phase plate based on the L-shaped nanoantenna for both linear and circular polarized incidences

    NASA Astrophysics Data System (ADS)

    Wang, Wei; Guo, Zhongyi; Sun, Yongxuan; Shen, Fei; Li, Yan; Liu, Yi; Wang, Xinshun; Qu, Shiliang

    2015-11-01

    Based on the L-shaped gold nanoantennas, the ultra-thin optical vortex phase plates (VPPs) have been designed to generate the optical vortex beams with different topological charges, which are independent of the incident polarization states and suitable for both X/Y linear and circular polarization incidences simultaneously. The phase and amplitude of transmitted cross-polarization light can be simultaneously manipulated by changing two degrees of freedom (the length and the width) in the L-shaped nanoantenna unit. Evolution properties of the generated vortex beam are demonstrated and analyzed. The different interactions of angular momentums between light and the VPP in the different incident polarization states have also been investigated fully. The designed VPP shows a superior broadband characteristics in near-infrared wavelength ranging from 750 nm to 1200 nm, which enable a potential implication for integrated optics and vortex optics.

  18. Quasi-phase-matching high harmonic generation using trains of pulses produced using an array of birefringent plates.

    PubMed

    O'Keeffe, Kevin; Robinson, Tom; Hooker, Simon M

    2012-03-12

    Quasi-phase-matched high harmonic generation using trains of up to 8 counter-propagating pulses is explored. For trains of up to 4 pulses the measured enhancement of the harmonic signal scales with the number of pulses N as (N + 1)², as expected. However, for trains with N > 4, no further enhancement of the harmonic signal is observed. This effect is ascribed to changes of the coherence length Lc within the generating medium. Techniques for overcoming the variation of Lc are discussed. The pressure dependence of quasi-phase-matching is investigated and the switch from true-phase-matching to quasi-phase-matching is observed.

  19. Thermal Lens Microscope

    NASA Astrophysics Data System (ADS)

    Uchiyama, Kenji; Hibara, Akihide; Kimura, Hiroko; Sawada, Tsuguo; Kitamori, Takehiko

    2000-09-01

    We developed a novel laser microscope based on the thermal lens effect induced by a coaxial beam comprised of excitation and probe beams. The signal generation mechanism was confirmed to be an authentic thermal lens effect from the measurement of signal and phase dependences on optical configurations between the sample and the probe beam focus, and therefore, the thermal lens effect theory could be applied. Two-point spatial resolution was determined by the spot size of the excitation beam, not by the thermal diffusion length. Sensitivity was quite high, and the detection ability, evaluated using a submicron microparticle containing dye molecules, was 0.8 zmol/μm2, hence a distribution image of trace chemical species could be obtained quantitatively. In addition, analytes are not restricted to fluorescent species, therefore, the thermal lens microscope is a promising analytical microscope. A two-dimensional image of a histamine molecule distribution, which was produced in mast cells at the femtomole level in a human nasal mucous polyp, was obtained.

  20. A Full-Field KB-FZP Microscope for Hard X-Ray Imaging with Sub 100 nm Resolution

    SciTech Connect

    Rau, C.; Crecea, V.; Peterson, K.M.; Jemian, P.R.; Richter, C.-P.; Neuhausler, U.; Schmeider, G.; Yu, X.; Braun, P.V.; Robinson, I.K.

    2007-06-28

    A full-field hard X-ray microscope has been built at the UNICAT/APS beamline 34ID-C. A Kirkpatrick-Baez mirror is used for the condenser and a micro-Fresnel Zone Plate (FZP) as the objective lens. The zone plates available give access to 50-85 nm spatial resolution operating the microscope between 6-12keV photon energy. The first tomography experiments have been performed with this device. A KB-FZP microscope has been built for sub-100 nm imaging and tomography. Features of 50 nm have been visualized at 9 keV photon energy. A 40 x 20 microns field of view of can be imaged in a minute. The first tomography experiments have been performed with this device. Further, it is planned to apply phase contrast techniques, such as the Zernike method. Both the efficiency and the resolution of the instrument can be further improved. A more efficient zone plate and an improved detector will reduce the exposure times and the use of the 50x100 times more intense so called 'pink-beam' is possible. To improve the resolution, the zone plates deliver in their third order a resolution of 15 nm. A KB-FZP microscope has been built for sub-100 nm imaging and tomography. Features of 50 nm have been visualized at 9 keV photon energy. A 40 x 20 microns field of view of can be imaged in seconds. Tomography experiments have been performed with this device. Phase objects have been visualized taking image series. Phase contrast techniques, such as the Zernike method will be tested in the future. Both the efficiency and the resolution of the instrument can be further improved. Together with the instrument for In-line phase contrast imaging the nano- and micrometer lenghtscale is covered.

  1. Scanning Microscopes Using X Rays and Microchannels

    NASA Technical Reports Server (NTRS)

    Wang, Yu

    2003-01-01

    Scanning microscopes that would be based on microchannel filters and advanced electronic image sensors and that utilize x-ray illumination have been proposed. Because the finest resolution attainable in a microscope is determined by the wavelength of the illumination, the xray illumination in the proposed microscopes would make it possible, in principle, to achieve resolutions of the order of nanometers about a thousand times as fine as the resolution of a visible-light microscope. Heretofore, it has been necessary to use scanning electron microscopes to obtain such fine resolution. In comparison with scanning electron microscopes, the proposed microscopes would likely be smaller, less massive, and less expensive. Moreover, unlike in scanning electron microscopes, it would not be necessary to place specimens under vacuum. The proposed microscopes are closely related to the ones described in several prior NASA Tech Briefs articles; namely, Miniature Microscope Without Lenses (NPO-20218), NASA Tech Briefs, Vol. 22, No. 8 (August 1998), page 43; and Reflective Variants of Miniature Microscope Without Lenses (NPO-20610), NASA Tech Briefs, Vol. 26, No. 9 (September 2002) page 6a. In all of these microscopes, the basic principle of design and operation is the same: The focusing optics of a conventional visible-light microscope are replaced by a combination of a microchannel filter and a charge-coupled-device (CCD) image detector. A microchannel plate containing parallel, microscopic-cross-section holes much longer than they are wide is placed between a specimen and an image sensor, which is typically the CCD. The microchannel plate must be made of a material that absorbs the illuminating radiation reflected or scattered from the specimen. The microchannels must be positioned and dimensioned so that each one is registered with a pixel on the image sensor. Because most of the radiation incident on the microchannel walls becomes absorbed, the radiation that reaches the

  2. Microscopic Rayleigh Droplet Beams

    NASA Astrophysics Data System (ADS)

    Doak, R. B.

    2005-11-01

    A periodically triggered Rayleigh Droplet Beam (RDB) delivers a perfectly linear and periodic stream of identical, monoenergetic droplets that are phase-locked to the trigger signal. The droplet diameter and spacing are easily adjusted of choice of nozzle diameter and trigger frequency. Any liquid of low viscosity may be emloyed as the beam fluid. Although the field of nanofluidics is expanding rapidly, little effort has yet been devoted to ``external flows'' such as RDB's. At ASU we have generated RDB's of water and methanol down to 2 microns in droplet diameter. Nozzle clogging is the sole impediment to smaller droplets. Microscopic Rayleigh droplet beams offer tremendous potential for fundamental physical measurements, fluid dynamics research, and nanofabrication. This talk will describe the apparatus and techniques used at ASU to generate RDB's (surprisingly simple and inexpensive), discuss the triboelectric phenomena that play a role (surprisingly significant), present some initial experimental fluid dynamics measurements, and briefly survey RDB applications. Our particular interest in RDB's is as microscopic transport systems to deliver hydrated, undenatured proteins into vacuum for structure determination via serial diffraction of x-rays or electrons. This may offer the first general method for structure determination of non-crystallizable proteins.

  3. Soft x-ray laser microscope

    SciTech Connect

    Suckewer, P.I.

    1990-10-01

    The program consisted of two phases (Phase I and Phase II). The goal of the Phase I (first year program) was to design and construct the Soft X-ray Laser Contact Microscope. Such microscope was constructed and adapted to PPL's 18.2nm soft X-ray Laser (SXL), which in turn was modified and prepared for microscopy experiments. Investigation of the photoresist response to 18.2nm laser radiation and transmissivity of 0.1m thick silicion-nitride (Si[sub 3]N[sub 4]) windows were important initial works. The goal of the first year of Phase II was to construct X-ray contact microscope in combination with existing optical phase microscope, already used by biologists. In the second year of Phase II study of dehydrated Horeseshoe Crab and Hela cancer cells were performed with COXRALM. Also during Phase II, the Imaging X-Ray Laser Microscope (IXRALM) was designed and constructed. This paper describes the development of each of the microscopes and their application for research.

  4. Involvement of the German Research Center for GeoSciences (GFZ) in the EPOS Implementation Phase 2015-18 (European Plate Observing System)

    NASA Astrophysics Data System (ADS)

    Hoffmann, T. L.; Lauterjung, J.

    2015-12-01

    Under the Horizon 2020 Programme INFRADEV-3, the European Commission (EC) has awarded a prioritized grant for the establishment of the European Plate Observing System (EPOS) during a four-year Implementation Phase 2015-18. As laid in detail during the EPOS Preparatory Phase 2010-14, the EPOS cyberinfrastructure will be established as an ERIC (European Research Infrastructure Consortium) and it will encompass the implementation of both the EPOS Integrated Core Services (ICS) for solid Earth Science and a multitude of EPOS Thematic Core Services (TCS). As one of the 29 awardees of the EC grant, the German Research Center for Geosciences (GFZ) will play an important role in the implementation of EPOS and its Thematic and Integrated Core Services. The presented poster will give an overview of GFZ's involvement in the different Work Packages, including administrative tasks (WP3 Harmonization) as well as the technical implementation efforts (WP7 ICS Development, WP8 Seismology, WP11 Volcano Observations, WP12 Satellite Data, WP13 Geomagnetic Observations, WP14 Anthropogenic Hazards, WP15 Geological Information and Modelling, WP16 Multi-Scale Laboratories and WP17 Geo Energy Test Beds).

  5. Characterizing cycle-to-cycle variations of the shedding cycle in the turbulent wake of a normal flat plate using generalized phase averages

    NASA Astrophysics Data System (ADS)

    Martinuzzi, Robert

    2016-11-01

    Quasi-periodic vortex shedding in the turbulent wake of a thin-flat plate placed normal to a uniform stream at Reynolds number of 6700 is investigated based on Particle Image Velocimetry experiments. The wake structure and vortex formation are characterized using a generalized phase average (GPA), a refinement of the triple decomposition of Reynolds and Hussain (1970) incorporating elements of mean-field theory (Stuart, 1958). The resulting analysis highlights the importance of cycle-to-cycle variations in characterizing vortex formation, wake topology and the residual turbulent Reynolds Stresses. For example, it is shown that during high-amplitude cycles vorticity is strongly concentrated within the well-organized shed vortices, whereas during low-amplitude cycles the shed vortices are highly distorted resulting in significant modulation of the shedding frequency. It is found that high-amplitude cycles contribute more to the coherent Reynolds stress field while the low-amplitude cycles contribute to the residual stress field. It is further shown that traditional phase-averaging techniques lead to an over-estimation of the residual stress field. Natural Sciences and Engineering Research Council of Canada.

  6. A high-throughput approach for the determination of pesticide residues in cucumber samples using solid-phase microextraction on 96-well plate.

    PubMed

    Bagheri, Habib; Es'haghi, Ali; Es-haghi, Ali; Mesbahi, Noushin

    2012-08-31

    A high-throughput solid-phase microextraction (SPME) on 96-well plate together with gas chromatography-mass spectrometry (GC-MS) was developed for the determination of some selected pesticides in cucumber samples. Pieces with the length of 1.0 cm of silicon tubing were precisely prepared and then coated on the end part of stainless steel wires. The prepared fibers were positioned in a home-made polytetrafluoroethylene (PTFE)-based constructed ninety-six holes block to have the possibility of simultaneous immersion of the SPME fibers into the center of individual wells. Pesticides such as diazinon, penconazol, tebuconazol, bitertanol, malathion, phosalone and chlorpyrifos-methyl were selected for their highly application in cucumber field. The performances of the SPME fibers, such as intra and inter-fibers reproducibility, were evaluated and the results showed a good similarity in extraction yields. A volume of 1 mL of the aquatic supernatant of the cucumber samples was transferred into the 96-well plate and the array of SPME fibers was applied for the extraction of the selected pesticides. The important parameters influencing the whole extraction process including, organic solvent percent, salt addition, dilution factor, stirring rate and extraction time were optimized. The inter- and intra-day RSD% were found to be less than 15.4%. Limits of detection (LOD) and limits of quantification (LOQ) were below 60 and 180 μg kg(-1), respectively. The coefficient of determination was satisfactory (r(2)>0.99) for all the studied analytes. The developed method was successfully applied to the monitoring of several samples gathered from local markets.

  7. Microscope and method of use

    DOEpatents

    Bongianni, Wayne L.

    1984-01-01

    A method and apparatus for electronically focusing and electronically scanning microscopic specimens are given. In the invention, visual images of even moving, living, opaque specimens can be acoustically obtained and viewed with virtually no time needed for processing (i.e., real time processing is used). And planar samples are not required. The specimens (if planar) need not be moved during scanning, although it will be desirable and possible to move or rotate nonplanar specimens (e.g., laser fusion targets) against the lens of the apparatus. No coupling fluid is needed, so specimens need not be wetted. A phase acoustic microscope is also made from the basic microscope components together with electronic mixers.

  8. Microscope and method of use

    DOEpatents

    Bongianni, W.L.

    1984-04-17

    A method and apparatus for electronically focusing and electronically scanning microscopic specimens are given. In the invention, visual images of even moving, living, opaque specimens can be acoustically obtained and viewed with virtually no time needed for processing (i.e., real time processing is used). And planar samples are not required. The specimens (if planar) need not be moved during scanning, although it will be desirable and possible to move or rotate nonplanar specimens (e.g., laser fusion targets) against the lens of the apparatus. No coupling fluid is needed, so specimens need not be wetted. A phase acoustic microscope is also made from the basic microscope components together with electronic mixers. 7 figs.

  9. Microscope and method of use

    SciTech Connect

    Bongianni, W.L.

    1981-08-18

    A method and apparatus for electronically focusing and electronically scanning microscopic specimens are given. In the invention, visual images of even moving, living, opaque specimens can be acoustically obtained and viewed with virtually no time needed for processing (i.e., real time processing is used). And planar samples are not required. The specimens (if planar) need not be moved during scanning, although it will be desirable and possible to move or rotate nonplanar specimens (e.g., laser fusion targets) against the lens of the apparatus. No coupling fluid is needed, so specimens need not be wetted. A phase acoustic microscope is also made from the basic microscope components together with electronic mixers.

  10. Microfluidic device enabled quantitative time-lapse microscopic-photography for phenotyping vegetative and reproductive phases in Fusarium virguliforme, which is pathogenic to soybean

    PubMed Central

    Marshall, Jill; Qiao, Xuan; Baumbach, Jordan; Xie, Jingyu; Dong, Liang; Bhattacharyya, Madan K.

    2017-01-01

    Time-lapse microscopic-photography allows in-depth phenotyping of microorganisms. Here we report development of such a system using a microfluidic device, generated from polydimethylsiloxane and glass slide, placed on a motorized stage of a microscope for conducting time-lapse microphotography of multiple observations in 20 channels simultaneously. We have demonstrated the utility of the device in studying growth, germination and sporulation in Fusarium virguliforme that causes sudden death syndrome in soybean. To measure the growth differences, we developed a polyamine oxidase fvpo1 mutant in this fungus that fails to grow in minimal medium containing polyamines as the sole nitrogen source. Using this system, we demonstrated that the conidiospores of the pathogen take an average of five hours to germinate. During sporulation, it takes an average of 10.5 h for a conidiospore to mature and get detached from its conidiophore for the first time. Conidiospores are developed in a single conidiophore one after another. The microfluidic device enabled quantitative time-lapse microphotography reported here should be suitable for screening compounds, peptides, micro-organisms to identify fungitoxic or antimicrobial agents for controlling serious plant pathogens. The device could also be applied in identifying suitable target genes for host-induced gene silencing in pathogens for generating novel disease resistance in crop plants. PMID:28295054

  11. Microfluidic device enabled quantitative time-lapse microscopic-photography for phenotyping vegetative and reproductive phases in Fusarium virguliforme, which is pathogenic to soybean.

    PubMed

    Marshall, Jill; Qiao, Xuan; Baumbach, Jordan; Xie, Jingyu; Dong, Liang; Bhattacharyya, Madan K

    2017-03-15

    Time-lapse microscopic-photography allows in-depth phenotyping of microorganisms. Here we report development of such a system using a microfluidic device, generated from polydimethylsiloxane and glass slide, placed on a motorized stage of a microscope for conducting time-lapse microphotography of multiple observations in 20 channels simultaneously. We have demonstrated the utility of the device in studying growth, germination and sporulation in Fusarium virguliforme that causes sudden death syndrome in soybean. To measure the growth differences, we developed a polyamine oxidase fvpo1 mutant in this fungus that fails to grow in minimal medium containing polyamines as the sole nitrogen source. Using this system, we demonstrated that the conidiospores of the pathogen take an average of five hours to germinate. During sporulation, it takes an average of 10.5 h for a conidiospore to mature and get detached from its conidiophore for the first time. Conidiospores are developed in a single conidiophore one after another. The microfluidic device enabled quantitative time-lapse microphotography reported here should be suitable for screening compounds, peptides, micro-organisms to identify fungitoxic or antimicrobial agents for controlling serious plant pathogens. The device could also be applied in identifying suitable target genes for host-induced gene silencing in pathogens for generating novel disease resistance in crop plants.

  12. TEAM Electron Microscope Animation

    SciTech Connect

    2012-01-01

    The TEAM Electron Microscope, a device that enables atomic-scale imaging in 3-D, has a rotating stage that can hold and position samples inside electron microscopes with unprecedented stability, position-control accuracy, and range of motion.The TEAM Stage makes one of the world's most powerful electron microscopes even better, and enables previously impossible experiments.

  13. Direct observation of electronic-liquid-crystal phase transitions and their microscopic origin in La1/3Ca2/3MnO3

    SciTech Connect

    Tao, J.; Sun, K.; Yin, W. -G.; Wu, L.; Xin, H.; Wen, J. G.; Luo, W.; Pennycook, S. J.; Tranquada, J. M.; Zhu, Y.

    2016-11-22

    The ground-state electronic order in doped manganites is frequently associated with a lattice modulation, contributing to their many interesting properties. However, measuring the thermal evolution of the lattice superstructure with reciprocal-space probes alone can lead to ambiguous results with competing interpretations. Here, we provide direct observations of the evolution of the superstructure in La1/3Ca2/3MnO3 in real space, as well as reciprocal space, using transmission electron microscopic (TEM) techniques. We show that the transitions are the consequence of a proliferation of dislocations plus electronic phase separation. The resulting states are well described by the symmetries associated with electronic-liquid-crystal (ELC) phases. Furthermore, our results resolve the long-standing controversy over the origin of the incommensurate superstructure and suggest a new structural model that is consistent with recent theoretical calculations.

  14. Peen plating

    NASA Technical Reports Server (NTRS)

    Babecki, A. J. (Inventor); Haehner, C. L.

    1973-01-01

    A process for metal plating which comprises spraying a mixture of metallic powder and small peening particles at high velocity against a surface is described. The velocity must be sufficient to impact and bond metallic powder onto the surface. In the case of metal surfaces, the process has as one of its advantages providing mechanical working (hardening) of the surface simultaneously with the metal plating.

  15. A new screening test for antimitotic compounds using the universal M phase-specific protein kinase, p34cdc2/cyclin Bcdc13, affinity-immobilized on p13suc1-coated microtitration plates.

    PubMed

    Rialet, V; Meijer, L

    1991-01-01

    A universal intracellular factor, the "M phase-Promoting Factor" (MPF), triggers the G2/M transition of the cell cycle in all organisms. This factor displays an easily assayable histone H1 kinase (H1K) activity and is composed of at least two subunits, p34cdc2 (catalytic) and cyclin Bcdc13 (regulatory). We describe here a microtitration plate assay using affinity-immobilized H1K-MPF as a cell cycle-specific target to screen for antimitotic compounds. First, meiotic starfish oocytes were selected as the most convenient and abundant source of M phase extracts containing high levels of H1K. Second, we used the strong and specific interaction between p34cdc2 and p13suc1 to affinity-immobilize H1K-MPF on p13suc1-coated microtitration plates. p13suc1-coated wells specifically retain the M phase kinase, the activity of which is assayed with histone H1 and gamma-32P-ATP. Among 10 microtitration plates, Maxisorp plates (Nunc) proved to be the most efficient at retaining H1K through p13suc1. Experimental conditions to coat the plates with p13suc1, to immobilize and to measure p34cdc2/cyclin Bcdc13 kinase activity, as well as to store p13suc1-precoated plates, have been optimized. Using this system we tested 18 currently used anticancer drugs and S or G2 inhibitors; none of them displayed any inhibitory activity. The microtitration assay has allowed the detection of two H1K inhibitors, isopentenyladenine (I50: 40 microM) and staurosporine (I50: 3.2 nM). This affinity-immobilized H1K-MPF can thus now be used as a simple screening system to detect inhibitors of a major cell cycle-regulating component. This method may prove useful to screen for antimitotic compounds of potential anticancer interest.

  16. Experimental and numerical analyses on a plate heat exchanger with phase change for waste heat recovery at off-design conditions

    NASA Astrophysics Data System (ADS)

    Cipollone, Roberto; Bianchi, Giuseppe; Di Battista, Davide; Fatigati, Fabio

    2015-11-01

    This paper analyzes the performances of an evaporator for small scale waste heat recovery applications based on bottoming Organic Rankine Cycles with net output power in the range 2-5 kW. The heat recovery steam generator is a plate heat exchanger with oil as hot stream and an organic fluid on the cold side. An experimental characterization of the heat exchanger was carried out at different operating points measuring temperatures, pressures and flow rates on both sides. The measurement data further allowed to validate a numerical model of the evaporator whereas heat transfer coefficients were evaluated comparing several literature correlations, especially for the phase-change of the organic fluid. With reference to a waste heat recovery application in industrial compressed air systems, multiple off-design conditions were simulated considering the effects of oil mass flow rate and temperature on the superheating of the organic fluid, a key parameter to ensure a proper operation of the expansion machine, thus of the energy recovery process.

  17. FIRST RESULTS FROM VERY LARGE TELESCOPE NACO APODIZING PHASE PLATE: 4 {mu}m IMAGES OF THE EXOPLANET {beta} PICTORIS b

    SciTech Connect

    Quanz, Sascha P.; Meyer, Michael R.; Kenworthy, Matthew A.; Girard, Julien H. V.; Kasper, Markus; Lagrange, Anne-Marie; Bonnefoy, Mickael; Chauvin, Gael; Apai, Daniel; Boccaletti, Anthony; Hinz, Philip M.; Lenzen, Rainer

    2010-10-10

    Direct imaging of exoplanets requires both high contrast and high spatial resolution. Here, we present the first scientific results obtained with the newly commissioned apodizing phase plate coronagraph (APP) on VLT/NACO. We detected the exoplanet {beta} Pictoris b in the narrowband filter centered at 4.05 {mu}m (NB4.05). The position angle (209.{sup 0}13 {+-} 2.{sup 0}12) and the projected separation to its host star (0.''354 {+-} 0.''012, i.e., 6.8 {+-} 0.2 AU at a distance of 19.3 pc) are in good agreement with the recently presented data from Lagrange et al. Comparing the observed NB4.05 magnitude of 11.20 {+-} 0.23 mag to theoretical atmospheric models, we find a best fit with a 7-10 M {sub Jupiter} object for an age of 12 Myr, again in agreement with previous estimates. Combining our results with published L' photometry, we can compare the planet's [L' - NB4.05] color to that of cool field dwarfs of higher surface gravity suggesting an effective temperature of {approx}1700 K. The best-fit theoretical model predicts an effective temperature of {approx}1470 K, but this difference is not significant given our photometric uncertainties. Our results demonstrate the potential of NACO/APP for future planet searches and provide independent confirmation as well as complementary data for {beta} Pic b.

  18. Diffraction of a finite-radius plane wave and a Gaussian beam by a helical axicon and a spiral phase plate.

    PubMed

    Kotlyar, Victor V; Kovalev, Alexey A; Skidanov, Roman V; Moiseev, Oleg Yu; Soifer, Victor A

    2007-07-01

    We derive what we believe to be new analytical relations to describe the Fraunhofer diffraction of the finite-radius plane wave by a helical axicon (HA) and a spiral phase plate (SPP). The solutions are deduced in the form of a series of the Bessel functions for the HA and a finite sum of the Bessel functions for the SPP. The solution for the HA changes to that for the SPP if the axicon parameter is set equal to zero. We also derive what we believe to be new analytical relations to describe the Fresnel and Fraunhofer diffraction of the Gaussian beam by a HA are derived. The solutions are deduced in the form of a series of the hypergeometric functions. We have fabricated by photolithography a binary diffractive optical element (a HA with number n=10) able to produce in the focal plane of a spherical lens an optical vortex, which was then used to perform rotation of several polystyrene beads of diameter 5 microm.

  19. MRF Applications: On the Road to Making Large-Aperture Ultraviolet Laser Resistant Continuous Phase Plates for High-Power Lasers

    SciTech Connect

    Menapace, J A; Davis, P J; Steele, W A; Hachkowski, M R; Nelson, A; Xin, K

    2006-10-26

    Over the past two years we have developed MRF tools and procedures to manufacture large-aperture (430 X 430 mm) continuous phase plates (CPPs) that are capable of operating in the infrared portion (1053 nm) of high-power laser systems. This is accomplished by polishing prescribed patterns of continuously varying topographical features onto finished plano optics using MRF imprinting techniques. We have been successful in making, testing, and using large-aperture CPPs whose topography possesses spatial periods as low as 4 mm and surface peak-to-valleys as high as 8.6 {micro}m. Combining this application of MRF technology with advanced MRF finishing techniques that focus on ultraviolet laser damage resistance makes it potentially feasible to manufacture large-aperture CPPs that can operate in the ultraviolet (351 nm) without sustaining laser-induced damage. In this paper, we will discuss the CPP manufacturing process and the results of 351-nm/3-nsec equivalent laser performance experiments conducted on large-aperture CPPs manufactured using advanced MRF protocols.

  20. A new approach to the application of solid phase extraction disks with LC-MS/MS for the analysis of drugs on a 96-well plate format.

    PubMed

    Cudjoe, Erasmus; Pawliszyn, Janusz

    2009-11-01

    A new 96-well disk solid phase extraction sample preparation technique which does not involve vacuum pumps integrated with liquid chromatographic tandem mass spectrometric (LC-MS/MS) was developed for high throughput determination of benzodiazepines (nordiazepam, diazepam, lorazepam and oxazepam). In addition, the method completely allows the re-use of the SPE disk membranes for subsequent analyses after re-conditioning. The method utilizes a robotic autosampler for parallel extractions in a 96-well plate format. Results have been presented for independent extractions from three matrices; phosphate buffer solution, urine, and plasma. Factors affecting data reproducibility, extraction kinetics, sample throughput, and reliability of the system were investigated and optimized. A total time required per sample was 0.94 min using 96-well format. Method reproducibility was < or =9% relative standard deviation for all three matrices. Limits of detection and quantitation recorded were respectively in the range 0.02-0.15 and 0.2-2.0 ng/mL with linearity ranging from 0.2 to 500 ng/mL for all matrices.

  1. Determination of ultrasonic wave velocities and phase velocity dispersion curves of an Inconel 600 plate using resonant ultrasound spectroscopy and leaky Lamb waves.

    PubMed

    Kim, Young H; Song, Sung-Jin; Kwon, Sung-Duk; Cheong, Yong-Moo; Jung, Hyun-Kyu

    2004-04-01

    A plate of Inconel 600 was interrogated using the resonant ultrasound spectroscopy (RUS) and the reflected leaky Lamb waves (LLW). It was found that the plate used in the present work has anisotropy in its material properties by the RUS. The longitudinal and the transverse wave velocities of the Inconel 600 plate were determined by the RUS, ultrasonic pulse-echo method and cut-off frequencies of the LLWs. The wave velocities in the direction of thickness determined by the RUS under the assumption of the orthotropic symmetry were quite similar to those obtained by other methods, the pulse-echo method and from cut-off frequencies. The reflected LLW from the plate was measured with varying the incident angle. The dispersion curves obtained from the reflected LLWs show good agreement with the theoretical calculation in general. The mismatches may be caused by anisotropy of the plate.

  2. Cryogenic immersion microscope

    SciTech Connect

    Le Gros, Mark; Larabell, Carolyn A.

    2010-12-14

    A cryogenic immersion microscope whose objective lens is at least partially in contact with a liquid reservoir of a cryogenic liquid, in which reservoir a sample of interest is immersed is disclosed. When the cryogenic liquid has an index of refraction that reduces refraction at interfaces between the lens and the sample, overall resolution and image quality are improved. A combination of an immersion microscope and x-ray microscope, suitable for imaging at cryogenic temperatures is also disclosed.

  3. Thermal-Wave Microscope

    NASA Technical Reports Server (NTRS)

    Jones, Robert E.; Kramarchuk, Ihor; Williams, Wallace D.; Pouch, John J.; Gilbert, Percy

    1989-01-01

    Computer-controlled thermal-wave microscope developed to investigate III-V compound semiconductor devices and materials. Is nondestructive technique providing information on subsurface thermal features of solid samples. Furthermore, because this is subsurface technique, three-dimensional imaging also possible. Microscope uses intensity-modulated electron beam of modified scanning electron microscope to generate thermal waves in sample. Acoustic waves generated by thermal waves received by transducer and processed in computer to form images displayed on video display of microscope or recorded on magnetic disk.

  4. Downgoing plate controls on overriding plate deformation in subduction zones

    NASA Astrophysics Data System (ADS)

    Garel, Fanny; Davies, Rhodri; Goes, Saskia; Davies, Huw; Kramer, Stephan; Wilson, Cian

    2014-05-01

    Although subduction zones are convergent margins, deformation in the upper plate can be extensional or compressional and tends to change through time, sometimes in repeated episodes of strong deformation, e.g, phases of back-arc extension. It is not well understood what factors control this upper plate deformation. We use the code Fluidity, which uses an adaptive mesh and a free-surface formulation, to model a two-plate subduction system in 2-D. The model includes a composite temperature- and stress-dependent rheology, and plates are decoupled by a weak layer, which allows for free trench motion. We investigate the evolution of the state of stress and topography of the overriding plate during the different phases of the subduction process: onset of subduction, free-fall sinking in the upper mantle and interaction of the slab with the transition zone, here represented by a viscosity contrast between upper and lower mantle. We focus on (i) how overriding plate deformation varies with subducting plate age; (ii) how spontaneous and episodic back-arc spreading develops for some subduction settings; (iii) the correlation between overriding plate deformation and slab interaction with the transition zone; (iv) whether these trends resemble observations on Earth.

  5. Simple versatile shearing interferometer suitable for measurements on a microscopic scale

    NASA Astrophysics Data System (ADS)

    Mihaylova, Emilia; Toal, Vincent

    2009-02-01

    Micro-electromechanical systems (MEMS) behave differently from massive samples. Conventional testing and inspection techniques usually fail at the microscale. Recently there has been an increasing interest in the application of optical techniques for microstructure testing, because they are high-resolution, non-contact, full-field, fast and relatively inexpensive. New interferometric systems, which are suitable for microscopic optical metrology, are of interest for engineering and industrial applications. A modified electronic speckle pattern shearing interferometer (ESPSI) with a very simple shearing device has been designed for metrology applications on the microscale. The shearing device consists of two partially reflective glass plates. The reflection coefficients of the coatings are 0.3 and 0.7, respectively. The distance and the tilt between the two glass plates control the size of the shear. A long working distance microscope objective is attached to the CCD camera to form a field of view variable over several millimetres in width. The suitability of the system for microscopic measurements is demonstrated. The capability of the system for phase shifting is also demonstrated. The results obtained are promising for future applications of the ESPSI system for testing and characterisation of MEMS.

  6. Development of x-ray laminography under an x-ray microscopic condition

    SciTech Connect

    Hoshino, Masato; Uesugi, Kentaro; Takeuchi, Akihisa; Suzuki, Yoshio; Yagi, Naoto

    2011-07-15

    An x-ray laminography system under an x-ray microscopic condition was developed to obtain a three-dimensional structure of laterally-extended planar objects which were difficult to observe by x-ray tomography. An x-ray laminography technique was introduced to an x-ray transmission microscope with zone plate optics. Three prototype sample holders were evaluated for x-ray imaging laminography. Layered copper grid sheets were imaged as a laminated sample. Diatomite powder on a silicon nitride membrane was measured to confirm the applicability of this method to non-planar micro-specimens placed on the membrane. The three-dimensional information of diatom shells on the membrane was obtained at a spatial resolution of sub-micron. Images of biological cells on the membrane were also obtained by using a Zernike phase contrast technique.

  7. Phase measurements of EUV mask defects

    DOE PAGES

    Claus, Rene A.; Wang, Yow-Gwo; Wojdyla, Antoine; ...

    2015-02-22

    Extreme Ultraviolet (EUV) Lithography mask defects were examined on the actinic mask imaging system, SHARP, at Lawrence Berkeley National Laboratory. Also, a quantitative phase retrieval algorithm based on the Weak Object Transfer Function was applied to the measured through-focus aerial images to examine the amplitude and phase of the defects. The accuracy of the algorithm was demonstrated by comparing the results of measurements using a phase contrast zone plate and a standard zone plate. Using partially coherent illumination to measure frequencies that would otherwise fall outside the numerical aperture (NA), it was shown that some defects are smaller than themore » conventional resolution of the microscope. We found that the programmed defects of various sizes were measured and shown to have both an amplitude and a phase component that the algorithm is able to recover.« less

  8. Phase measurements of EUV mask defects

    SciTech Connect

    Claus, Rene A.; Wang, Yow-Gwo; Wojdyla, Antoine; Benk, Markus P.; Goldberg, Kenneth A.; Neureuther, Andrew R.; Naulleau, Patrick P.; Waller, Laura

    2015-02-22

    Extreme Ultraviolet (EUV) Lithography mask defects were examined on the actinic mask imaging system, SHARP, at Lawrence Berkeley National Laboratory. Also, a quantitative phase retrieval algorithm based on the Weak Object Transfer Function was applied to the measured through-focus aerial images to examine the amplitude and phase of the defects. The accuracy of the algorithm was demonstrated by comparing the results of measurements using a phase contrast zone plate and a standard zone plate. Using partially coherent illumination to measure frequencies that would otherwise fall outside the numerical aperture (NA), it was shown that some defects are smaller than the conventional resolution of the microscope. We found that the programmed defects of various sizes were measured and shown to have both an amplitude and a phase component that the algorithm is able to recover.

  9. Optical Near-Field Plates

    DTIC Science & Technology

    2015-04-08

    microscopy images of seven plasmonic color filters illuminated by the white microscope light. Scale bar is 10 µm. (b) Experimentally measured transmission... color filtering and spectral imaging ,” Nat. Comm. 1, 59 (2010). 3. H.-F. Shi and L. J. Guo, “Design of Plasmonic Near Field Plate at Opitical...wavelength. Plasmonic filters were developed and transmission color filtering was accomplished. The polarization property and the pitch-dependent

  10. Photography through the Microscope.

    ERIC Educational Resources Information Center

    McNeil, D. W.

    1992-01-01

    Describes how to illuminate and optically stain slides for microscope use and how to interface a 35mm camera with a microscope using an adaptor. Provides equipment descriptions and sources, details about illumination, image formation, darkfield adaptors, centerable filter adaptors, darkfield stops, rheinburg filters, and choosing specimens to…

  11. Mailing microscope slides

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many insects feed agriculturally important crops, trees, and ornamental plants and cause millions of dollars of damage annually. Identification for some of these require the preparation of a microscope slide for examination. There are times when a microscope slide may need to be sent away to a speci...

  12. The Homemade Microscope.

    ERIC Educational Resources Information Center

    Baker, Roger C., Jr.

    1991-01-01

    Directions for the building of a pocket microscope that will make visible the details of insect structure and living bacteria are described. Background information on the history of microscopes and lenses is provided. The procedures for producing various types of lenses are included. (KR)

  13. Optical Analysis of Microscope Images

    NASA Astrophysics Data System (ADS)

    Biles, Jonathan R.

    Microscope images were analyzed with coherent and incoherent light using analog optical techniques. These techniques were found to be useful for analyzing large numbers of nonsymbolic, statistical microscope images. In the first part phase coherent transparencies having 20-100 human multiple myeloma nuclei were simultaneously photographed at 100 power magnification using high resolution holographic film developed to high contrast. An optical transform was obtained by focussing the laser onto each nuclear image and allowing the diffracted light to propagate onto a one dimensional photosensor array. This method reduced the data to the position of the first two intensity minima and the intensity of successive maxima. These values were utilized to estimate the four most important cancer detection clues of nuclear size, shape, darkness, and chromatin texture. In the second part, the geometric and holographic methods of phase incoherent optical processing were investigated for pattern recognition of real-time, diffuse microscope images. The theory and implementation of these processors was discussed in view of their mutual problems of dimness, image bias, and detector resolution. The dimness problem was solved by either using a holographic correlator or a speckle free laser microscope. The latter was built using a spinning tilted mirror which caused the speckle to change so quickly that it averaged out during the exposure. To solve the bias problem low image bias templates were generated by four techniques: microphotography of samples, creation of typical shapes by computer graphics editor, transmission holography of photoplates of samples, and by spatially coherent color image bias removal. The first of these templates was used to perform correlations with bacteria images. The aperture bias was successfully removed from the correlation with a video frame subtractor. To overcome the limited detector resolution it is necessary to discover some analog nonlinear intensity

  14. This-layer chromatography/electrospray ionization triple-quadrupole linear ion trap mass spectrometry system: analysis of rhodamine dyes separated on reversed-phase C8 plates

    SciTech Connect

    Ford, Michael J; Kertesz, Vilmos; Van Berkel, Gary J

    2005-01-01

    The direct analysis of separated rhodamine dyes on reversed-phase C{sub 8} thin-layer chromatography plates using a surface sampling/electrospray emitter probe coupled with a triple-quadrupole linear ion trap mass spectrometer is presented. This report represents continuing work to advance the performance metrics and utility of this basic surface sampling electrospray mass spectrometry system for the analysis of thin-layer chromatography plates. Experimental results examining the role of sampling probe spray end configuration on liquid aspiration rate and gas-phase ion signal generated are discussed. The detection figures-of-merit afforded by full-scan, automated product ion and selected reaction monitoring modes of operation were examined. The effect of different eluting solvents on mass spectrum signal levels with the reversed-phase C{sub 8} plate was investigated. The combined effect of eluting solvent flow-rate and development lane surface scan rate on preservation of chromatographic resolution was also studied. Analysis of chromatographically separated red pen ink extracts from eight different pens using selected reaction monitoring demonstrated the potential of this surface sampling electrospray mass spectrometry system for targeted compound analysis with real samples.

  15. Plate electronics

    NASA Astrophysics Data System (ADS)

    Carlowicz, Michael

    Using a Cray T3D supercomputer and a simple assumption about the physical character of Earth's mantle, a pair of researchers from the University of California at Berkeley have built a computer model that may help explain why the planet's tectonic plates look the way they do.In creating a three-dimensional numerical simulation of convection in the Earth's interior, UC researchers Hans-Peter Bunge and Mark Richards simplified their model to account for just one major physical effect: that the viscosity of the mantle increases with depth. Reviewing some recent—but not yet widely accepted—seismic data, Bunge and Richards assumed for the sake of the model that the viscosity of the mantle increases by a factor of 30 from the lithosphere to the core-mantle boundary. Relying on that assumption, the pair ran the model for nearly three weeks on a supercomputer at Los Alamos National Laboratory and found that the simulation produced an effect similar to what we see on the surface of Earth. The model produced a surface paralleling the actual width of plates and the geometry of the plate boundaries.

  16. Assessment of Petrological Microscopes.

    ERIC Educational Resources Information Center

    Mathison, Charter Innes

    1990-01-01

    Presented is a set of procedures designed to check the design, ergonomics, illumination, function, optics, accessory equipment, and image quality of a microscope being considered for purchase. Functions for use in a petrology or mineralogy laboratory are stressed. (CW)

  17. Microscope collision protection apparatus

    DOEpatents

    DeNure, Charles R.

    2001-10-23

    A microscope collision protection apparatus for a remote control microscope which protects the optical and associated components from damage in the event of an uncontrolled collision with a specimen, regardless of the specimen size or shape. In a preferred embodiment, the apparatus includes a counterbalanced slide for mounting the microscope's optical components. This slide replaces the rigid mounts on conventional upright microscopes with a precision ball bearing slide. As the specimen contacts an optical component, the contacting force will move the slide and the optical components mounted thereon. This movement will protect the optical and associated components from damage as the movement causes a limit switch to be actuated, thereby stopping all motors responsible for the collision.

  18. Microscopic tubes in igneous rocks

    NASA Technical Reports Server (NTRS)

    Richter, D.; Simmons, G.

    1977-01-01

    Microscopic tubes have been observed in several igneous rocks and may be quite common. They occur in single crystals and have either elliptical or circular cross-sections 1 to 5 microns in diameter and are ten to hundreds of microns long. Microtubes may be hollow or partially or completely filled with another phase, but are distinct from acicular crystals of accessory minerals such as rutile. Microtubes can form by at least three processes: (1) the partial annealing of microcracks, (2) the natural etching of dislocations, or (3) the primary inclusion of fluid material during crystal growth.

  19. Hypoxia in Microscopic Tumors

    PubMed Central

    Li, Xiao-Feng; O’Donoghue, Joseph A

    2008-01-01

    Tumor hypoxia has been commonly observed in a broad spectrum of primary solid malignancies. Hypoxia is associated with tumor progression, increased aggressiveness, enhanced metastatic potential and poor prognosis. Hypoxic tumor cells are resistant to radiotherapy and some forms of chemotherapy. Using an animal model, we recently showed that microscopic tumors less than 1 mm diameter were severely hypoxic. In this review, models and techniques for the study of hypoxia in microscopic tumors are discussed. PMID:18384940

  20. Infrared microscope inspection apparatus

    DOEpatents

    Forman, Steven E.; Caunt, James W.

    1985-02-26

    Apparatus and system for inspecting infrared transparents, such as an array of photovoltaic modules containing silicon solar cells, includes an infrared microscope, at least three sources of infrared light placed around and having their axes intersect the center of the object field and means for sending the reflected light through the microscope. The apparatus is adapted to be mounted on an X-Y translator positioned adjacent the object surface.

  1. Infrared microscope inspection apparatus

    DOEpatents

    Forman, S.E.; Caunt, J.W.

    1985-02-26

    Apparatus and system for inspecting infrared transparents, such as an array of photovoltaic modules containing silicon solar cells, includes an infrared microscope, at least three sources of infrared light placed around and having their axes intersect the center of the object field and means for sending the reflected light through the microscope. The apparatus is adapted to be mounted on an X-Y translator positioned adjacent the object surface. 4 figs.

  2. Phase contrast in high resolution electron microscopy

    DOEpatents

    Rose, H.H.

    1975-09-23

    This patent relates to a device for developing a phase contrast signal for a scanning transmission electron microscope. The lens system of the microscope is operated in a condition of defocus so that predictable alternate concentric regions of high and low electron density exist in the cone of illumination. Two phase detectors are placed beneath the object inside the cone of illumination, with the first detector having the form of a zone plate, each of its rings covering alternate regions of either higher or lower electron density. The second detector is so configured that it covers the regions of electron density not covered by the first detector. Each detector measures the number of electrons incident thereon and the signal developed by the first detector is subtracted from the signal developed by the record detector to provide a phase contrast signal. (auth)

  3. An evaluation of the use of a commercial scanner to obtain experimental data produced by gas-phase electron diffraction and recorded on photographic plates

    NASA Astrophysics Data System (ADS)

    Aarset, Kirsten; Hagen, Kolbjørn; Page, Elizabeth M.; Rice, David A.

    1999-03-01

    A commercial scanner (Agfa Arcus II) has been used to retrieve electron-diffraction data from photographic plates. The data thus obtained from five different molecules has been analysed and the results compared with the original published data. Excellent agreement was observed between bond distances and amplitudes obtained from refinements on data collected from this scanner, a similar scanner and a micro-densitometer. It is planned to use the Agfa Arcus II scanner for future measurement of electron-diffraction intensity data from photographic plates.

  4. Plating Waste Sludge Metal Recovery.

    DTIC Science & Technology

    1985-05-30

    l - ~ ’.4 41 *4 7.1 4...34 L €5 ’ ’ . ’ " " r ,*: -: ,., -. . .,,:€ ,, .. € : ....- -1. PHASE I - UERATURE REVIEW Phase I of the project "Plating Waste Sludge Metal Recovery...8217’’,. ’.’.."..-’......’ ...’ , m .m llll- l ..m~ l : j nm~, l ~linn~ . l , b~~h~h , . r - r. -*1 . * , * . . , .d PHASE IV -

  5. Intermittent Plate Tectonics

    NASA Astrophysics Data System (ADS)

    Silver, P. G.; Behn, M. D.

    2006-12-01

    Intermittent Plate Tectonics A basic premise of Earth Science is that plate tectonics has been continuously operating since it began early in Earth's history. Yet, plate-tectonic theory itself, specifically the collisional phase of the Wilson Cycle, constitutes a process that is capable of stopping all plate motion. The plausibility of a plate-tectonic hiatus is most easily illustrated by considering the expected future of the present-day plate-tectonic configuration. Since the opening of the Atlantic at ~200 ma, the area of the Atlantic basin has been growing at the expense of the Pacific. If this trend continues, relative plate motion models predict that in ~350 my, the Pacific Ocean basin will effectively close leading to widespread continent-continent collisions. Since a continent-continent collision represents the termination of subduction locally, the accumulated effect of all collisions is to stop subduction globally. In this scenario, ridges would then stop spreading and young oceanic lithosphere would cool, reaching a steady-state thickness of 100 km in about 80 my, based on the properties of oceanic lithosphere today. This would constitute the stoppage of plate tectonics. The presumption that plate tectonics never stops in the face of continental collisions is equivalent to requiring that subduction flux is approximately constant through time, such that subduction initiation roughly balances subduction termination. Such a balance then raises several questions about the subduction initiation process. When and how does subduction initiate? Is there a detectible relationship between subduction cessation and subduction initiation? We can gain some guidance into these questions by examining the plate motion history over the last 200 my. Subduction initiation has occurred over the last 80 my in three intra- oceanic subduction zones: Aleutians, Marianas-Izu-Bonin and Tonga-Kermadec in the Pacific basin. In these cases, however, subduction initiation would not

  6. The Scanning Optical Microscope: An Overview

    NASA Astrophysics Data System (ADS)

    Kino, G. S.; Corte, T. R.; Xiao, G. Q.

    1988-07-01

    In the last few years there has been a resurgence in research on optical microscopes. One reason stems from the invention of the acoustic microscope by Quate and Lemons,1 and the realization that some of the same principles could be applied to the optical microscope. The acoustic microscope has better transverse definition for the same wavelength than the standard optical microscope and at the same time has far better range definition. Consequently, Kompfner, who was involved with the work on the early acoustic microscope, decided to try out similar scanning microscope principles with optics, and started a group with Wilson and Sheppard to carry out such research at Oxford.2 Sometime earlier, Petran et a13 had invented the tandem scanning microscope which used many of the same principles. Now, in our laboratory at Stanford, these ideas on the tandem scanning microscope and the scanning optical microscope are converging. Another aspect of this work, which stems from the earlier experience with the acoustic microscope, involves measurement of both phase and amplitude of the optical beam. It is also possible to use scanned optical microscopy for other purposes. For instance, an optical beam can be used to excite electrons and holes in semiconductors, and the generated current can be measured. By scanning the optical beam over the semiconductor, an image can be obtained of the regions where there is strong or weak electron hole generation. This type of microscope is called OBIC (Optical Beam Induced Current). A second application involves fluorescent imaging of biological materials. Here we have the excellent range definition of a scanning optical microscope which eliminates unwanted glare from regions of the material where the beam is unfocused.3 A third application is focused on the heating effect of the light beam. With such a system, images can be obtained which are associated with changes in the thermal properties of a material, changes in recombination rates in

  7. Electron microscope studies

    SciTech Connect

    Crewe, A.V.; Kapp, O.H.

    1992-07-01

    This is a report covering the research performed in the Crewe laboratory between 1964 and 1992. Because of limitations of space we have provided relatively brief summaries of the major research directions of the facility during these years. A complete bibliography has been included and we have referenced groups of pertinent publications at the beginning of each section. This report summarizes our efforts to develop better electron microscopes and chronicles many of the experimental programs, in materials science and biology, that acted both as a stimulus to better microscope design and also as a testing ground for many instrumental innovations.

  8. Links between microscopic and macroscopic fluid mechanics

    NASA Astrophysics Data System (ADS)

    Hoover, Wm. G.; Hoover, C. G.

    2003-01-01

    The microscopic and macroscopic versions of fluid mechanics differ qualitatively. Microscopic particles obey time-reversible ordinary differential equations. The resulting particle trajectories {q(t)} may be time-averaged or ensemble-averaged so as to generate field quantities corresponding to macroscopic variables. On the other hand, the macroscopic continuum fields described by fluid mechanics follow irreversible partial differential equations. Smooth particle methods bridge the gap separating these two views of fluids by solving the macroscopic field equations with particle dynamics that resemble molecular dynamics. Recently, nonlinear dynamics have provided some useful tools for understanding the relationship between the microscopic and macroscopic points of view. Chaos and fractals play key roles in this new understanding. Non-equilibrium phase-space averages look very different from their equilibrium counterparts. Away from equilibrium the smooth phase-space distributions are replaced by fractional-dimensional singular distributions that exhibit time irreversibility.

  9. Gain and far-field patterns for phase-correcting Fresnel zone plate antennas at millimeter-wave and terahertz frequencies

    NASA Astrophysics Data System (ADS)

    Wiltse, James C.

    2007-04-01

    The Fresnel zone plate lens antenna, which provides advantages compared to a normal paraboloidal or spherical lens, has been extensively investigated in the millimeter-wave and terahertz regions. The advantages include reduced weight, volume, and attenuation and simplicity of design. The principal disadvantage is that the zone plate sometimes provides reduced gain compared to a true lens. Particularly at high millimeter-wave or terahertz frequencies the low loss of the zone plate more than compensates for the reduced directivity. This paper investigates the gains and far-field patterns for a number of cases and gives both the analysis and numerical results for the examples. These cases have dealt with large-angle designs, where the focal length (F) and diameter (D) are comparable (F/D = 0.3 to 2.5), unlike the typical optical examples. The antenna patterns are found to have beamwidths and first sidelobes that are similar to what one would obtain with a standard lens, given the same aperture illumination. Appropriate feed designs are also described. For best aperture efficiency the illumination taper is about 10 dB, and this gives first sidelobe levels of about -24dB for a circular aperture. Far-out average sidelobes are not as low as for a true lens, and this is where the gain is affected.

  10. Microscope on Mars

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This image taken at Meridiani Planum, Mars by the panoramic camera on the Mars Exploration Rover Opportunity shows the rover's microscopic imager (circular device in center), located on its instrument deployment device, or 'arm.' The image was acquired on the ninth martian day or sol of the rover's mission.

  11. Making Art with Microscopes

    ERIC Educational Resources Information Center

    Benedis-Grab, Gregory

    2011-01-01

    Interdisciplinary teaching is a great way to focus on overarching concepts and help students make connections across disciplines. Historically, art and science have been connected disciplines. The botanical prints of the 18th and 19th centuries and early work with microscopes are two examples of a need for strong artistic skills in the science…

  12. Shock compression dynamics under a microscope

    NASA Astrophysics Data System (ADS)

    Dlott, Dana D.

    2017-01-01

    Our laboratory has developed a tabletop laser miniflyer launcher used for a wide variety of studies in the physical and chemical sciences. The flyers, typically 0.7 mm in diameter, can be used to shock microgram quantities of interesting materials. Frequently 100 shock experiments per day are performed. A microscope objective transmits the photon Doppler velocimeter (PDV) flyer plate diagnostic and various laser beams, and collects signals from the shocked materials that can be transmitted to video cameras, spectrographs, streak cameras, etc. In this paper I describe the flyer plate apparatus and then discuss three recent efforts: (1) Shock dissipation in nanoporous media; (2) Probing micropressures in shocked microstructured media; and (3) Shock initiation of nanotechnology reactive materials.

  13. X-ray laser microscope apparatus

    DOEpatents

    Suckewer, Szymon; DiCicco, Darrell S.; Hirschberg, Joseph G.; Meixler, Lewis D.; Sathre, Robert; Skinner, Charles H.

    1990-01-01

    A microscope consisting of an x-ray contact microscope and an optical microscope. The optical, phase contrast, microscope is used to align a target with respect to a source of soft x-rays. The source of soft x-rays preferably comprises an x-ray laser but could comprise a synchrotron or other pulse source of x-rays. Transparent resist material is used to support the target. The optical microscope is located on the opposite side of the transparent resist material from the target and is employed to align the target with respect to the anticipated soft x-ray laser beam. After alignment with the use of the optical microscope, the target is exposed to the soft x-ray laser beam. The x-ray sensitive transparent resist material whose chemical bonds are altered by the x-ray beam passing through the target mater GOVERNMENT LICENSE RIGHTS This invention was made with government support under Contract No. De-FG02-86ER13609 awarded by the Department of Energy. The Government has certain rights in this invention.

  14. IMIS: An intelligence microscope imaging system

    NASA Technical Reports Server (NTRS)

    Caputo, Michael; Hunter, Norwood; Taylor, Gerald

    1994-01-01

    Until recently microscope users in space relied on traditional microscopy techniques that required manual operation of the microscope and recording of observations in the form of written notes, drawings, or photographs. This method was time consuming and required the return of film and drawings from space for analysis. No real-time data analysis was possible. Advances in digital and video technologies along with recent developments in article intelligence will allow future space microscopists to have a choice of three additional modes of microscopy: remote coaching, remote control, and automation. Remote coaching requires manual operations of the microscope with instructions given by two-way audio/video transmission during critical phases of the experiment. When using the remote mode of microscopy, the Principal Investigator controls the microscope from the ground. The automated mode employs artificial intelligence to control microscope functions and is the only mode that can be operated in the other three modes as well. The purpose of this presentation is to discuss the advantages and disadvantages of the four modes of of microscopy and how the IMIS, a proposed intelligent microscope imaging system, can be used as a model for developing and testing concepts, operating procedures, and equipment design of specifications required to provide a comprehensive microscopy/imaging capability onboard Space Station Freedom.

  15. Zernike Phase Contrast Electron Cryo-Tomography Applied to Marine Cyanobacteria Infected with Cyanophages

    PubMed Central

    Dai, Wei; Fu, Caroline; Khant, Htet A.; Ludtke, Steven J.; Schmid, Michael F.; Chiu, Wah

    2015-01-01

    Advances in electron cryo-tomography have provided a new opportunity to visualize the internal 3D structures of a bacterium. An electron microscope equipped with Zernike phase contrast optics produces images with dramatically increased contrast compared to images obtained by conventional electron microscopy. Here we describe a protocol to apply Zernike phase plate technology for acquiring electron tomographic tilt series of cyanophage-infected cyanobacterial cells embedded in ice, without staining or chemical fixation. We detail the procedures for aligning and assessing phase plates for data collection, and methods to obtain 3D structures of cyanophage assembly intermediates in the host, by subtomogram alignment, classification and averaging. Acquiring three to four tomographic tilt series takes approximately 12 h on a JEM2200FS electron microscope. We expect this time requirement to decrease substantially as the technique matures. Time required for annotation and subtomogram averaging varies widely depending on the project goals and data volume. PMID:25321408

  16. Microscopic features of moving traffic jams

    NASA Astrophysics Data System (ADS)

    Kerner, Boris S.; Klenov, Sergey L.; Hiller, Andreas; Rehborn, Hubert

    2006-04-01

    Empirical and numerical microscopic features of moving traffic jams are presented. Based on a single vehicle data analysis, it is found that within wide moving jams, i.e., between the upstream and downstream jam fronts there is a complex microscopic spatiotemporal structure. This jam structure consists of alternations of regions in which traffic flow is interrupted and flow states of low speeds associated with “moving blanks” within the jam. Moving blanks within a wide moving jam resemble electron holes in the valence band of semiconductors: As the moving blanks that propagate upstream appear due to downstream vehicle motion within the jam, so appearance of electron holes moving with the electric field results from electron motion against the electric field in the valence band of semiconductors. Empirical features of moving blanks are found. Based on microscopic models in the context of the Kerner’s three-phase traffic theory, physical reasons for moving blanks emergence within wide moving jams are disclosed. Microscopic nonlinear effects of moving jam emergence, propagation, and dissolution as well as a diverse variety of hysteresis effects in freeway traffic associated with phase transitions and congested traffic propagation are numerically investigated. Microscopic structure of moving jam fronts is numerically studied and compared with empirical results.

  17. Corrugated cover plate for flat plate collector

    DOEpatents

    Hollands, K. G. Terry; Sibbitt, Bruce

    1978-01-01

    A flat plate radiant energy collector is providing having a transparent cover. The cover has a V-corrugated shape which reduces the amount of energy reflected by the cover away from the flat plate absorber of the collector.

  18. Growth Plate Fractures

    MedlinePlus

    .org Growth Plate Fractures Page ( 1 ) The bones of children and adults share many of the same risks for injury. But because they ... to a unique injury called a growth plate fracture. Growth plates are areas of cartilage located near ...

  19. Ion photon emission microscope

    DOEpatents

    Doyle, Barney L.

    2003-04-22

    An ion beam analysis system that creates microscopic multidimensional image maps of the effects of high energy ions from an unfocussed source upon a sample by correlating the exact entry point of an ion into a sample by projection imaging of the ion-induced photons emitted at that point with a signal from a detector that measures the interaction of that ion within the sample. The emitted photons are collected in the lens system of a conventional optical microscope, and projected on the image plane of a high resolution single photon position sensitive detector. Position signals from this photon detector are then correlated in time with electrical effects, including the malfunction of digital circuits, detected within the sample that were caused by the individual ion that created these photons initially.

  20. Femtosecond scanning tunneling microscope

    SciTech Connect

    Taylor, A.J.; Donati, G.P.; Rodriguez, G.; Gosnell, T.R.; Trugman, S.A.; Some, D.I.

    1998-11-01

    This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). By combining scanning tunneling microscopy with ultrafast optical techniques we have developed a novel tool to probe phenomena on atomic time and length scales. We have built and characterized an ultrafast scanning tunneling microscope in terms of temporal resolution, sensitivity and dynamic range. Using a novel photoconductive low-temperature-grown GaAs tip, we have achieved a temporal resolution of 1.5 picoseconds and a spatial resolution of 10 nanometers. This scanning tunneling microscope has both cryogenic and ultra-high vacuum capabilities, enabling the study of a wide range of important scientific problems.

  1. Soft x-ray laser microscope. Final report

    SciTech Connect

    Suckewer, P.I.

    1990-10-01

    The program consisted of two phases (Phase I and Phase II). The goal of the Phase I (first year program) was to design and construct the Soft X-ray Laser Contact Microscope. Such microscope was constructed and adapted to PPL`s 18.2nm soft X-ray Laser (SXL), which in turn was modified and prepared for microscopy experiments. Investigation of the photoresist response to 18.2nm laser radiation and transmissivity of 0.1m thick silicion-nitride (Si{sub 3}N{sub 4}) windows were important initial works. The goal of the first year of Phase II was to construct X-ray contact microscope in combination with existing optical phase microscope, already used by biologists. In the second year of Phase II study of dehydrated Horeseshoe Crab and Hela cancer cells were performed with COXRALM. Also during Phase II, the Imaging X-Ray Laser Microscope (IXRALM) was designed and constructed. This paper describes the development of each of the microscopes and their application for research.

  2. Color Laser Microscope

    NASA Astrophysics Data System (ADS)

    Awamura, D.; Ode, T.; Yonezawa, M.

    1987-04-01

    A color laser microscope utilizing a new color laser imaging system has been developed for the visual inspection of semiconductors. The light source, produced by three lasers (Red; He-Ne, Green; Ar, Blue; He-Cd), is deflected horizontally by an AOD (Acoustic Optical Deflector) and vertically by a vibration mirror. The laser beam is focused in a small spot which is scanned over the sample at high speed. The light reflected back from the sample is reformed to contain linear information by returning to the original vibration mirror. The linear light is guided to the CCD image sensor where it is converted into a video signal. Individual CCD image sensors are used for each of the three R, G, or B color image signals. The confocal optical system with its laser light source yields a color TV monitor image with no flaring and a much sharper resolution than that of the conventional optical microscope. The AOD makes possible a high speed laser scan and a NTSC or PAL TV video signal is produced in real time without any video memory. Since the light source is composed of R, G, and B laser beams, color separation superior to that of white light illumination is achieved. Because of the photometric linearity of the image detector, the R, G, and B outputs of the system are most suitably used for hue analysis. The CCD linear image sensors in the optical system produce no geometrical distortion, and good color registration is available principally. The output signal can be used for high accuracy line width measuring. The many features of the color laser microscope make it ideally suited for the visual inspection of semiconductor processing. A number of these systems have already been installed in such a capacity. The Color Laser Microscope can also be a very useful tool for the fields of material engineering and biotechnology.

  3. Microscopic enteritis: Bucharest consensus.

    PubMed

    Rostami, Kamran; Aldulaimi, David; Holmes, Geoffrey; Johnson, Matt W; Robert, Marie; Srivastava, Amitabh; Fléjou, Jean-François; Sanders, David S; Volta, Umberto; Derakhshan, Mohammad H; Going, James J; Becheanu, Gabriel; Catassi, Carlo; Danciu, Mihai; Materacki, Luke; Ghafarzadegan, Kamran; Ishaq, Sauid; Rostami-Nejad, Mohammad; Peña, A Salvador; Bassotti, Gabrio; Marsh, Michael N; Villanacci, Vincenzo

    2015-03-07

    Microscopic enteritis (ME) is an inflammatory condition of the small bowel that leads to gastrointestinal symptoms, nutrient and micronutrient deficiency. It is characterised by microscopic or sub-microscopic abnormalities such as microvillus changes and enterocytic alterations in the absence of definite macroscopic changes using standard modern endoscopy. This work recognises a need to characterize disorders with microscopic and submicroscopic features, currently regarded as functional or non-specific entities, to obtain further understanding of their clinical relevance. The consensus working party reviewed statements about the aetiology, diagnosis and symptoms associated with ME and proposes an algorithm for its investigation and treatment. Following the 5(th) International Course in Digestive Pathology in Bucharest in November 2012, an international group of 21 interested pathologists and gastroenterologists formed a working party with a view to formulating a consensus statement on ME. A five-step agreement scale (from strong agreement to strong disagreement) was used to score 21 statements, independently. There was strong agreement on all statements about ME histology (95%-100%). Statements concerning diagnosis achieved 85% to 100% agreement. A statement on the management of ME elicited agreement from the lowest rate (60%) up to 100%. The remaining two categories showed general agreement between experts on clinical presentation (75%-95%) and pathogenesis (80%-90%) of ME. There was strong agreement on the histological definition of ME. Weaker agreement on management indicates a need for further investigations, better definitions and clinical trials to produce quality guidelines for management. This ME consensus is a step toward greater recognition of a significant entity affecting symptomatic patients previously labelled as non-specific or functional enteropathy.

  4. Virtual pinhole confocal microscope

    SciTech Connect

    George, J.S.; Rector, D.M.; Ranken, D.M.; Peterson, B.; Kesteron, J.

    1999-06-01

    Scanned confocal microscopes enhance imaging capabilities, providing improved contrast and image resolution in 3-D, but existing systems have significant technical shortcomings and are expensive. Researchers at Los Alamos National Laboratory have developed a novel approach--virtual pinhole confocal microscopy--that uses state of the art illumination, detection, and data processing technologies to produce an imager with a number of advantages: reduced cost, faster imaging, improved efficiency and sensitivity, improved reliability and much greater flexibility. Work at Los Alamos demonstrated proof of principle; prototype hardware and software have been used to demonstrate technical feasibility of several implementation strategies. The system uses high performance illumination, patterned in time and space. The authors have built functional confocal imagers using video display technologies (LCD or DLP) and novel scanner based on a micro-lens array. They have developed a prototype system for high performance data acquisition and processing, designed to support realtime confocal imaging. They have developed algorithms to reconstruct confocal images from a time series of spatially sub-sampled images; software development remains an area of active development. These advances allow the collection of high quality confocal images (in fluorescence, reflectance and transmission modes) with equipment that can inexpensively retrofit to existing microscopes. Planned future extensions to these technologies will significantly enhance capabilities for microscopic imaging in a variety of applications, including confocal endoscopy, and confocal spectral imaging.

  5. Thimble microscope system

    NASA Astrophysics Data System (ADS)

    Kamal, Tahseen; Rubinstein, Jaden; Watkins, Rachel; Cen, Zijian; Kong, Gary; Lee, W. M.

    2016-12-01

    Wearable computing devices, e.g. Google Glass, Smart watch, embodies the new human design frontier, where technology interfaces seamlessly with human gestures. During examination of any subject in the field (clinic, surgery, agriculture, field survey, water collection), our sensory peripherals (touch and vision) often go hand-in-hand. The sensitivity and maneuverability of the human fingers are guided with tight distribution of biological nerve cells, which perform fine motor manipulation over a range of complex surfaces that is often out of sight. Our sight (or naked vision), on the other hand, is generally restricted to line of sight that is ill-suited to view around corner. Hence, conventional imaging methods are often resort to complex light guide designs (periscope, endoscopes etc) to navigate over obstructed surfaces. Using modular design strategies, we constructed a prototype miniature microscope system that is incorporated onto a wearable fixture (thimble). This unique platform allows users to maneuver around a sample and take high resolution microscopic images. In this paper, we provide an exposition of methods to achieve a thimble microscopy; microscope lens fabrication, thimble design, integration of miniature camera and liquid crystal display.

  6. Ultra high frequency imaging acoustic microscope

    DOEpatents

    Deason, Vance A.; Telschow, Kenneth L.

    2006-05-23

    An imaging system includes: an object wavefront source and an optical microscope objective all positioned to direct an object wavefront onto an area of a vibrating subject surface encompassed by a field of view of the microscope objective, and to direct a modulated object wavefront reflected from the encompassed surface area through a photorefractive material; and a reference wavefront source and at least one phase modulator all positioned to direct a reference wavefront through the phase modulator and to direct a modulated reference wavefront from the phase modulator through the photorefractive material to interfere with the modulated object wavefront. The photorefractive material has a composition and a position such that interference of the modulated object wavefront and modulated reference wavefront occurs within the photorefractive material, providing a full-field, real-time image signal of the encompassed surface area.

  7. Autophagy: a new phase in the maturation of growth plate chondrocytes is regulated by HIF, mTOR and AMP kinase.

    PubMed

    Srinivas, Vickram; Bohensky, Jolene; Shapiro, Irving M

    2009-01-01

    The overall goal of the investigation was to examine autophagy in the growth plate and to ascertain how this process was regulated. Herein, we show that in the postmitotic maturing zone of the growth plate, chondrocytes express an autophagic phenotype. This robust and particulate immunohistochemical response provides direct evidence that autophagy is a new and transient stage in the chondrocyte maturation pathway. We found that induction of autophagy was regulated by mTOR, a sensor of cellular metabolism. When mTOR was inhibited, changes in LC3 fluorescence indicated that this kinase regulated development of the autophagic state. To determine if AMP kinase was required for chondrocyte autophagy, we suppressed its expression in N1511 cells using siRNA technology. When these cells were serum starved, a condition that triggers autophagy, there was no change in LC3 distribution. This result confirmed that AMP kinase was required for the induction of the autophagic response. Based on the 2 studies described above, and our previous observation that HIF-1 is required for the induction of autophagy, we put forward the hypothesis that autophagy is regulated by the activities of AMP kinase and mTOR in a HIF-1-dependent manner. Once autophagy is activated, the postmitotic chondrocytes would be expected to remain viable in their unique microenvironment and complete their life cycle.

  8. Wave-Based Inversion & Imaging for the Optical Quadrature Microscope

    SciTech Connect

    Lehman, S K

    2005-10-27

    The Center for Subsurface Sensing & Imaging System's (CenSSIS) Optical Quadrature Microscope (OQM) is a narrow band visible light microscope capable of measuring both amplitude and phase of a scattered field. We develop a diffraction tomography, that is, wave-based, scattered field inversion and imaging algorithm, for reconstructing the refractive index of the scattering object.

  9. Q: How do Microscopes Work?

    ERIC Educational Resources Information Center

    Zimov, Sarah

    2004-01-01

    Microscopes allow scientists to examine everyday objects in extraordinary ways. They provide high-resolution images that show objects in fine detail. This brief article describes the many types of microscopes and how they are used in different scientific venues.

  10. Constructing a multi-scan synchrotron X-ray microscope to study the function of osteocyte canaliculi in mouse bone

    SciTech Connect

    Nango, Nobuhito; Kubota, Shogo; Yashiro, Wataru; Momose, Atsushi; Takada, Yasunari; Matsuo, Koichi

    2012-07-31

    Formulating a multi-scan method applied to an X-ray microscope CT with synchrotron radiation, we attempted to analyze the 3D functional structure of osteocyte canaliculi inside the cortical bone of a mouse tibia. We employed a two-method combination to scan the same position of the specimen. To extract the internal bone canalicular structure, we first combined a Talbot interferometer with an X-ray microscope, and applied a differential phase imaging method to measure the absolute value of bone mineral around the canaliculi. Next, we used the X-ray microscope without the Talbot interferometer under a defocus condition, moving the specimen toward the zone plate by 6 mm. This defocus contrast method visualizes the canaliculi by emphasizing the edges of the bone. We performed CT scans by the two configurations and precisely aligned resultant 3D images so that the same position in the specimen is compared. We could extract the osteocyte canaliculi and evaluate the mineral density of their surroundings. The degree of mineralization varied for each osteocyte lacuna and canaliculus. The multi-scan microscopic X-ray CT is a powerful tool for analyzing bone mineralization.

  11. Constructing a multi-scan synchrotron X-ray microscope to study the function of osteocyte canaliculi in mouse bone

    NASA Astrophysics Data System (ADS)

    Nango, Nobuhito; Kubota, Shogo; Yashiro, Wataru; Momose, Atsushi; Takada, Yasunari; Matsuo, Koichi

    2012-07-01

    Formulating a multi-scan method applied to an X-ray microscope CT with synchrotron radiation, we attempted to analyze the 3D functional structure of osteocyte canaliculi inside the cortical bone of a mouse tibia. We employed a two-method combination to scan the same position of the specimen. To extract the internal bone canalicular structure, we first combined a Talbot interferometer with an X-ray microscope, and applied a differential phase imaging method to measure the absolute value of bone mineral around the canaliculi. Next, we used the X-ray microscope without the Talbot interferometer under a defocus condition, moving the specimen toward the zone plate by 6 mm. This defocus contrast method visualizes the canaliculi by emphasizing the edges of the bone. We performed CT scans by the two configurations and precisely aligned resultant 3D images so that the same position in the specimen is compared. We could extract the osteocyte canaliculi and evaluate the mineral density of their surroundings. The degree of mineralization varied for each osteocyte lacuna and canaliculus. The multi-scan microscopic X-ray CT is a powerful tool for analyzing bone mineralization.

  12. Adirondack Under the Microscope

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This image was taken by the Mars Exploration Rover Spirit front hazard-identification camera after the rover's first post-egress drive on Mars Sunday, Jan. 15, 2004. Engineers drove the rover approximately 3 meters (10 feet) from the Columbia Memorial Station toward the first rock target, seen in the foreground. The football-sized rock was dubbed Adirondack because of its mountain-shaped appearance. Scientists have begun using the microscopic imager instrument at the end of the rover's robotic arm to examine the rock and understand how it formed.

  13. Solid state optical microscope

    SciTech Connect

    Young, Ian T.

    1983-01-01

    A solid state optical microscope wherein wide-field and high-resolution images of an object are produced at a rapid rate by utilizing conventional optics with a charge-coupled photodiode array. A galvanometer scanning mirror, for scanning in one of two orthogonal directions is provided, while the charge-coupled photodiode array scans in the other orthogonal direction. Illumination light from the object is incident upon the photodiodes, creating packets of electrons (signals) which are representative of the illuminated object. The signals are then processed, stored in a memory, and finally displayed as a video signal.

  14. Solid state optical microscope

    DOEpatents

    Young, I.T.

    1983-08-09

    A solid state optical microscope wherein wide-field and high-resolution images of an object are produced at a rapid rate by utilizing conventional optics with a charge-coupled photodiode array. A galvanometer scanning mirror, for scanning in one of two orthogonal directions is provided, while the charge-coupled photodiode array scans in the other orthogonal direction. Illumination light from the object is incident upon the photodiodes, creating packets of electrons (signals) which are representative of the illuminated object. The signals are then processed, stored in a memory, and finally displayed as a video signal. 2 figs.

  15. Low frequency acoustic microscope

    DOEpatents

    Khuri-Yakub, Butrus T.

    1986-11-04

    A scanning acoustic microscope is disclosed for the detection and location of near surface flaws, inclusions or voids in a solid sample material. A focused beam of acoustic energy is directed at the sample with its focal plane at the subsurface flaw, inclusion or void location. The sample is scanned with the beam. Detected acoustic energy specularly reflected and mode converted at the surface of the sample and acoustic energy reflected by subsurface flaws, inclusions or voids at the focal plane are used for generating an interference signal which is processed and forms a signal indicative of the subsurface flaws, inclusions or voids.

  16. Atomic Force Microscope

    SciTech Connect

    Day, R.D.; Russell, P.E.

    1988-12-01

    The Atomic Force Microscope (AFM) is a recently developed instrument that has achieved atomic resolution imaging of both conducting and non- conducting surfaces. Because the AFM is in the early stages of development, and because of the difficulty of building the instrument, it is currently in use in fewer than ten laboratories worldwide. It promises to be a valuable tool for obtaining information about engineering surfaces and aiding the .study of precision fabrication processes. This paper gives an overview of AFM technology and presents plans to build an instrument designed to look at engineering surfaces.

  17. Sputtering and ion plating

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The proceedings of a conference on sputtering and ion plating are presented. Subjects discussed are: (1) concepts and applications of ion plating, (2) sputtering for deposition of solid film lubricants, (3) commercial ion plating equipment, (4) industrial potential for ion plating and sputtering, and (5) fundamentals of RF and DC sputtering.

  18. Comparison of turbulent-flow chromatography with automated solid-phase extraction in 96-well plates and liquid-liquid extraction used as plasma sample preparation techniques for liquid chromatography-tandem mass spectrometry.

    PubMed

    Zimmer, D; Pickard, V; Czembor, W; Müller, C

    1999-08-27

    Turbulent flow chromatography (TFC) combined with the high selectivity and sensitivity of tandem mass spectrometry (MS-MS) is a new technique for the fast direct analysis of drugs from crude plasma. TFC in the 96-well plate format reduces significantly the time required for sample clean-up in the laboratory. For example, for 100 samples the workload for a technician is reduced from about 8 h by a manual liquid-liquid extraction (LLE) assay to about 1 h in the case of TFC. Sample clean-up and analysis are performed on-line on the same column. Similar chromatographic performance and validation results were achieved using HTLC Turbo-C18 columns (Cohesive Technologies) and Oasis HLB extraction columns (Waters). One 96-well plate with 96 plasma samples is analyzed within 5.25 h, corresponding to 3.3 min per sample. Compared to this LLE and analysis of 96 samples takes about 16 h. Two structurally different and highly protein bound compounds, drug A and drug B, were analyzed under identical TFC conditions and the assays were fully validated for the application to toxicokinetics studies (compliant with Good Laboratory Practices-GLP). The limit of quantitation was 1.00 microg/l and the linear working range covered three orders of magnitude for both drugs. In the case of drug A the quality of analysis by TFC was similar to the reference LLE assay and slightly better than automated solid-phase extraction in 96-well plates. The accuracy was -3.1 to 6.7% and the precision was 3.1 to 6.8% in the case of drug A determined for dog plasma by TFC-MS-MS. For drug B the accuracy was -3.7 to 3.5% and the precision was 1.6 to 5.4% for rat plasma, which is even slightly better than what was achieved with the validated protein precipitation assay.

  19. Battery plate containing filler with conductive coating

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor)

    1985-01-01

    The plate (10) comprises a matrix or binder resin phase (12) in which is dispersed particulate, conductive tin oxide such as tin oxide coated glass fibers (14). A monopolar plate (11) is prepared by coating a layer (18) of electrolytically active material onto a surface of the plate (10). Tin oxide is prevented from reduction by coating a surface of the plate (10) with a conductive, impervious layer resistant to reduction such as a thin film (22) of lead adhered to the plate with a layer (21) of conductive adhesive. The plate (10) can be formed by casting a molten dispersion from mixer (36) onto a sheet (30) of lead foil or by passing an assembly of a sheet (41) of resin, a sheet (43) of fiberglass and a sheet (45) of lead between the nip of heated rollers (48, 50).

  20. Battery plate containing filler with conductive coating

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor)

    1986-01-01

    The plate (10) comprises a matrix or binder resin phase (12) in which is dispersed particulate, conductive tin oxide such as tin oxide coated glass fibers (14). A monopolar plate (11) is prepared by coating a layer (18) of electrolytically active material onto a surface of the plate (10). Tin oxide is prevented from reduction by coating a surface of the plate (10) with a conductive, impervious layer resistant to reduction such as a thin film (22) of lead adhered to the plate with a layer (21) of conductive adhesive. The plate (10) can be formed by casting a molten dispersion from mixer (36) onto a sheet (30) of lead foil or by passing an assembly of a sheet (41) of resin, a sheet (43) of fiberglass and a sheet (45) of lead between the nip of heated rollers (48, 50).

  1. Forensic Scanning Electron Microscope

    NASA Astrophysics Data System (ADS)

    Keeley, R. H.

    1983-03-01

    The scanning electron microscope equipped with an x-ray spectrometer is a versatile instrument which has many uses in the investigation of crime and preparation of scientific evidence for the courts. Major applications include microscopy and analysis of very small fragments of paint, glass and other materials which may link an individual with a scene of crime, identification of firearms residues and examination of questioned documents. Although simultaneous observation and chemical analysis of the sample is the most important feature of the instrument, other modes of operation such as cathodoluminescence spectrometry, backscattered electron imaging and direct x-ray excitation are also exploited. Marks on two bullets or cartridge cases can be compared directly by sequential scanning with a single beam or electronic linkage of two instruments. Particles of primer residue deposited on the skin and clothing when a gun is fired can be collected on adhesive tape and identified by their morphology and elemental composition. It is also possible to differentiate between the primer residues of different types of ammunition. Bullets may be identified from the small fragments left behind as they pass through the body tissues. In the examination of questioned documents the scanning electron microscope is used to establish the order in which two intersecting ink lines were written and to detect traces of chemical markers added to the security inks on official documents.

  2. Microscopic Tribotactic Walkers

    NASA Astrophysics Data System (ADS)

    Steimel, Joshua; Aragones, Juan; Alexander-Katz, Alfredo

    2014-03-01

    The translational motion of a rotating object near a surface is strongly dependent on the friction between the object and the surface. The process of friction is inherently directional and the friction coefficient can be anisotropic even in the absence of a net friction coefficient gradient. This is macroscopically observed in the ordering motif of some animal hair or scales and a microscopic analog can be imagined where the friction coefficient is determined by the strength and density of reversible bonds between a rotating object and the substrate. For high friction coefficients most of the rotational motion is converted into translational motion; conversely for low friction coefficients the object primarily rotates in place. We exploited this property to design and test a new class of motile system that displays tribotaxis, which is the process by which an object detects differences in the local friction coefficient and moves accordingly either to regions of higher or lower friction. These synthetic tribotactic microscopic walkers, composed of a pair of functionalized superparamagnetic beads, detect gradients in the spatial friction coefficient and migrate towards high friction areas when actuated in a random fashion. The effective friction between the walkers and the substrate is controlled by the local density of active receptors in the substrate. The tribotactic walkers also displayed trapping in high friction areas where the density of free receptors is higher.

  3. Plate mode velocities in graphite/epoxy plates

    NASA Technical Reports Server (NTRS)

    Prosser, W. H.; Gorman, M. R.

    1994-01-01

    Measurements of the velocities of the extensional and flexural plate modes were made along three directions of propagation in four graphite/epoxy composite plates. The acoustic signals were generated by simulated acoustic emission events (pencil lead breaks or Hsu-Neilson sources) and detected by by broadband ultrasonic transducers. The first arrival of the extensional plate mode, which is nondispersive at low frequencies, was measured at a number of different distances from the source along the propagation direction of interest. The velocity was determined by plotting the distance versus arrival time and computing its slope. Because of the large dispersion of the flexural mode, a Fourier phase velocity technique was used to characterize this mode. The velocity was measured up to a frequency of 160 kHz. Theoretical predictions of the velocities of these modes were also made and compared with experimental observations. Classical plate theory yields good agreement with the measured extensional velocities. For predictions of the dispersion of the flexural mode, Mindlin plates theory, which includes the effects of shear deformation and rotatory inertia was shown to give better agreement with the experimental measurements.

  4. Embryos, microscopes, and society.

    PubMed

    Maienschein, Jane

    2016-06-01

    Embryos have different meanings for different people and in different contexts. Seen under the microscope, the biological embryo starts out as one cell and then becomes a bunch of cells. Gradually these divide and differentiate to make up the embryo, which in humans becomes a fetus at eight weeks, and then eventually a baby. At least, that happens in those cases that carry through normally and successfully. Yet a popular public perception imagines the embryo as already a little person in the very earliest stages of development, as if it were predictably to become an adult. In actuality, cells can combine, pull apart, and recombine in a variety of ways and still produce embryos, whereas most embryos never develop into adults at all. Biological embryos and popular imaginations of embryos diverge. This paper looks at some of the historical reasons for and social implications of that divergence.

  5. Mars Under the Microscope

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This magnified look at the martian soil near the Mars Exploration Rover Opportunity's landing site, Meridiani Planum, shows coarse grains sprinkled over a fine layer of sand. The image was captured by the rover's microscopic imager on the 10th day, or sol, of its mission. Scientists are intrigued by the spherical rocks, which can be formed by a variety of geologic processes, including cooling of molten lava droplets and accretion of concentric layers of material around a particle or 'seed.'

    The examined patch of soil is 3 centimeters (1.2 inches) across. The circular grain in the lower left corner is approximately 3 millimeters (.12 inches) across, or about the size of a sunflower seed.

  6. Imaging arrangement and microscope

    SciTech Connect

    Pertsinidis, Alexandros; Chu, Steven

    2015-12-15

    An embodiment of the present invention is an imaging arrangement that includes imaging optics, a fiducial light source, and a control system. In operation, the imaging optics separate light into first and second tight by wavelength and project the first and second light onto first and second areas within first and second detector regions, respectively. The imaging optics separate fiducial light from the fiducial light source into first and second fiducial light and project the first and second fiducial light onto third and fourth areas within the first and second detector regions, respectively. The control system adjusts alignment of the imaging optics so that the first and second fiducial light projected onto the first and second detector regions maintain relatively constant positions within the first and second detector regions, respectively. Another embodiment of the present invention is a microscope that includes the imaging arrangement.

  7. Atomic Force Microscope Operation

    NASA Technical Reports Server (NTRS)

    2008-01-01

    [figure removed for brevity, see original site] Click on image for animation (large file)

    This animation is a scientific illustration of the operation of NASA's Phoenix Mars Lander's Atomic Force Microscope, or AFM. The AFM is part of Phoenix's Microscopy, Electrochemistry, and Conductivity Analyzer, or MECA.

    The AFM is used to image the smallest Martian particles using a very sharp tip at the end of one of eight beams.

    The beam of the AFM is set into vibration and brought up to the surface of a micromachined silicon substrate. The substrate has etched in it a series of pits, 5 micrometers deep, designed to hold the Martian dust particles.

    The microscope then maps the shape of particles in three dimensions by scanning them with the tip.

    At the end of the animation is a 3D representation of the AFM image of a particle that was part of a sample informally called 'Sorceress.' The sample was delivered to the AFM on the 38th Martian day, or sol, of the mission (July 2, 2008).

    The image shows four round pits, only 5 microns in depth, that were micromachined into the silicon substrate.

    A Martian particle only one micrometer, or one millionth of a meter, across is held in the upper left pit.

    The rounded particle shown at the highest magnification ever seen from another world is a particle of the dust that cloaks Mars. Such dust particles color the Martian sky pink, feed storms that regularly envelop the planet and produce Mars' distinctive red soil.

    The AFM was developed by a Swiss-led consortium, with Imperial College London producing the silicon substrate that holds sampled particles.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  8. Acoustic microscope surface inspection system and method

    DOEpatents

    Khuri-Yakub, Butrus T.; Parent, Philippe; Reinholdtsen, Paul A.

    1991-01-01

    An acoustic microscope surface inspection system and method in which pulses of high frequency electrical energy are applied to a transducer which forms and focuses acoustic energy onto a selected location on the surface of an object and receives energy from the location and generates electrical pulses. The phase of the high frequency electrical signal pulses are stepped with respected to the phase of a reference signal at said location. An output signal is generated which is indicative of the surface of said selected location. The object is scanned to provide output signals representative of the surface at a plurality of surface locations.

  9. Acoustic microscope surface inspection system and method

    DOEpatents

    Khuri-Yakub, B.T.; Parent, P.; Reinholdtsen, P.A.

    1991-02-26

    An acoustic microscope surface inspection system and method are described in which pulses of high frequency electrical energy are applied to a transducer which forms and focuses acoustic energy onto a selected location on the surface of an object and receives energy from the location and generates electrical pulses. The phase of the high frequency electrical signal pulses are stepped with respect to the phase of a reference signal at said location. An output signal is generated which is indicative of the surface of said selected location. The object is scanned to provide output signals representative of the surface at a plurality of surface locations. 7 figures.

  10. High thermal capacity cold plate/hot plate

    NASA Technical Reports Server (NTRS)

    Hwangbo, H.; Mcever, W. S.

    1985-01-01

    The results of an analytical study to determine the feasibility of a novel two-phase cold plate/hot plate (CPHP) are presented. A key feature of the CPHP is the use of capillary forces to separate the liquid and vapor phases and distribute the liquid over the evaporating/condensing surface. The liquid phase is held by capillary forces in a reservoir and is carried to the evaporating surface by a wick. The reservoir is replenished at intervals from a valved external liquid supply line. In the hot plate mode, liquid accumulates in the reservoir and is removed by an external condensate line. Performance requirements for the device were capability of handling a power density of 4 watts/sq cm, an outlet quality (percentage of vapor flow to total flow) greater than 90 percent, and operation in a 0-g environment. An analytical model of CPHP operation was developed which concentrated on the liquid and vapor flows. It was found that the liquid carrying capillary grooves of rectangular cross-section gave significantly better predicted performance than V-shaped grooves. Using the analytical model, capillary groove width and length, vapor channel dimensions, and other parameters were selected to meet the performance requirements with a 100-percent margin.

  11. Femtosecond photoelectron point projection microscope

    SciTech Connect

    Quinonez, Erik; Handali, Jonathan; Barwick, Brett

    2013-10-15

    By utilizing a nanometer ultrafast electron source in a point projection microscope we demonstrate that images of nanoparticles with spatial resolutions of the order of 100 nanometers can be obtained. The duration of the emission process of the photoemitted electrons used to make images is shown to be of the order of 100 fs using an autocorrelation technique. The compact geometry of this photoelectron point projection microscope does not preclude its use as a simple ultrafast electron microscope, and we use simple analytic models to estimate temporal resolutions that can be expected when using it as a pump-probe ultrafast electron microscope. These models show a significant increase in temporal resolution when comparing to ultrafast electron microscopes based on conventional designs. We also model the microscopes spectroscopic abilities to capture ultrafast phenomena such as the photon induced near field effect.

  12. Proper alignment of the microscope.

    PubMed

    Rottenfusser, Rudi

    2013-01-01

    The light microscope is merely the first element of an imaging system in a research facility. Such a system may include high-speed and/or high-resolution image acquisition capabilities, confocal technologies, and super-resolution methods of various types. Yet more than ever, the proverb "garbage in-garbage out" remains a fact. Image manipulations may be used to conceal a suboptimal microscope setup, but an artifact-free image can only be obtained when the microscope is optimally aligned, both mechanically and optically. Something else is often overlooked in the quest to get the best image out of the microscope: Proper sample preparation! The microscope optics can only do its job when its design criteria are matched to the specimen or vice versa. The specimen itself, the mounting medium, the cover slip, and the type of immersion medium (if applicable) are all part of the total optical makeup. To get the best results out of a microscope, understanding the functions of all of its variable components is important. Only then one knows how to optimize these components for the intended application. Different approaches might be chosen to discuss all of the microscope's components. We decided to follow the light path which starts with the light source and ends at the camera or the eyepieces. To add more transparency to this sequence, the section up to the microscope stage was called the "Illuminating Section", to be followed by the "Imaging Section" which starts with the microscope objective. After understanding the various components, we can start "working with the microscope." To get the best resolution and contrast from the microscope, the practice of "Koehler Illumination" should be understood and followed by every serious microscopist. Step-by-step instructions as well as illustrations of the beam path in an upright and inverted microscope are included in this chapter. A few practical considerations are listed in Section 3.

  13. Transmission electron microscope CCD camera

    DOEpatents

    Downing, Kenneth H.

    1999-01-01

    In order to improve the performance of a CCD camera on a high voltage electron microscope, an electron decelerator is inserted between the microscope column and the CCD. This arrangement optimizes the interaction of the electron beam with the scintillator of the CCD camera while retaining optimization of the microscope optics and of the interaction of the beam with the specimen. Changing the electron beam energy between the specimen and camera allows both to be optimized.

  14. Earth's Decelerating Tectonic Plates

    SciTech Connect

    Forte, A M; Moucha, R; Rowley, D B; Quere, S; Mitrovica, J X; Simmons, N A; Grand, S P

    2008-08-22

    Space geodetic and oceanic magnetic anomaly constraints on tectonic plate motions are employed to determine a new global map of present-day rates of change of plate velocities. This map shows that Earth's largest plate, the Pacific, is presently decelerating along with several other plates in the Pacific and Indo-Atlantic hemispheres. These plate decelerations contribute to an overall, globally averaged slowdown in tectonic plate speeds. The map of plate decelerations provides new and unique constraints on the dynamics of time-dependent convection in Earth's mantle. We employ a recently developed convection model constrained by seismic, geodynamic and mineral physics data to show that time-dependent changes in mantle buoyancy forces can explain the deceleration of the major plates in the Pacific and Indo-Atlantic hemispheres.

  15. Desktop water window microscope using a double-stream gas puff target source

    NASA Astrophysics Data System (ADS)

    Wachulak, Przemyslaw W.; Torrisi, Alfio; Bartnik, Andrzej; Adjei, Daniel; Kostecki, Jerzy; Wegrzynski, Lukasz; Jarocki, Roman; Szczurek, Mirosław; Fiedorowicz, Henryk

    2015-03-01

    A compact, desktop size, microscope, based on nitrogen double-stream gas puff target soft X-ray source, which emits radiation in water window spectral range at the wavelength of λ = 2.88 nm, is demonstrated. The microscope employs ellipsoidal grazing incidence condenser mirror for sample illumination and Fresnel zone plate objective. The microscope is capable of capturing magnified images of objects with 60 nm spatial resolution and exposure time as low as a few seconds. Details about the source and the microscope as well as a few examples of different applications are presented and discussed.

  16. Spectral Interferometry with Electron Microscopes.

    PubMed

    Talebi, Nahid

    2016-09-21

    Interference patterns are not only a defining characteristic of waves, but also have several applications; characterization of coherent processes and holography. Spatial holography with electron waves, has paved the way towards space-resolved characterization of magnetic domains and electrostatic potentials with angstrom spatial resolution. Another impetus in electron microscopy has been introduced by ultrafast electron microscopy which uses pulses of sub-picosecond durations for probing a laser induced excitation of the sample. However, attosecond temporal resolution has not yet been reported, merely due to the statistical distribution of arrival times of electrons at the sample, with respect to the laser time reference. This is however, the very time resolution which will be needed for performing time-frequency analysis. These difficulties are addressed here by proposing a new methodology to improve the synchronization between electron and optical excitations through introducing an efficient electron-driven photon source. We use focused transition radiation of the electron as a pump for the sample. Due to the nature of transition radiation, the process is coherent. This technique allows us to perform spectral interferometry with electron microscopes, with applications in retrieving the phase of electron-induced polarizations and reconstructing dynamics of the induced vector potential.

  17. Microscopic theory of rubber elasticity.

    PubMed

    Oyerokun, Folusho T; Schweizer, Kenneth S

    2004-05-15

    A microscopic integral equation theory of elasticity in polymer liquids and networks is developed which addresses the nonclassical problem of the consequences of interchain repulsive interactions and packing correlations on mechanical response. The theory predicts strain induced softening, and a nonclassical intermolecular contribution to the linear modulus. The latter is of the same magnitude as the classical single chain entropy contribution at low polymer concentrations, but becomes much more important in the melt state, and dominant as the isotropic-nematic liquid crystal phase transition is approached. Comparison of the calculated stress-strain curve and induced nematic order parameter with computer simulations show good agreement. A nearly quadratic dependence of the linear elastic modulus on segmental concentration is found, as well as a novel fractional power law dependence on degree of polymerization. Quantitative comparison of the theory with experiments on polydimethylsiloxane networks are presented and good agreement is found. However, a nonzero modulus in the long chain limit is not predicted since quenched chemical crosslinks and trapped entanglements are not explicitly taken into account. The theory is generalizable to treat the structure, thermodynamics and mechanical response of nematic elastomers.

  18. Spectral Interferometry with Electron Microscopes

    PubMed Central

    Talebi, Nahid

    2016-01-01

    Interference patterns are not only a defining characteristic of waves, but also have several applications; characterization of coherent processes and holography. Spatial holography with electron waves, has paved the way towards space-resolved characterization of magnetic domains and electrostatic potentials with angstrom spatial resolution. Another impetus in electron microscopy has been introduced by ultrafast electron microscopy which uses pulses of sub-picosecond durations for probing a laser induced excitation of the sample. However, attosecond temporal resolution has not yet been reported, merely due to the statistical distribution of arrival times of electrons at the sample, with respect to the laser time reference. This is however, the very time resolution which will be needed for performing time-frequency analysis. These difficulties are addressed here by proposing a new methodology to improve the synchronization between electron and optical excitations through introducing an efficient electron-driven photon source. We use focused transition radiation of the electron as a pump for the sample. Due to the nature of transition radiation, the process is coherent. This technique allows us to perform spectral interferometry with electron microscopes, with applications in retrieving the phase of electron-induced polarizations and reconstructing dynamics of the induced vector potential. PMID:27649932

  19. Accelerated plate tectonics.

    PubMed

    Anderson, D L

    1975-03-21

    The concept of a stressed elastic lithospheric plate riding on a viscous asthenosphere is used to calculate the recurrence interval of great earthquakes at convergent plate boundaries, the separation of decoupling and lithospheric earthquakes, and the migration pattern of large earthquakes along an arc. It is proposed that plate motions accelerate after great decoupling earthquakes and that most of the observed plate motions occur during short periods of time, separated by periods of relative quiescence.

  20. Athena microscopic Imager investigation

    USGS Publications Warehouse

    Herkenhoff, K. E.; Squyres, S. W.; Bell, J.F.; Maki, J.N.; Arneson, H.M.; Bertelsen, P.; Brown, D.I.; Collins, S.A.; Dingizian, A.; Elliott, S.T.; Goetz, W.; Hagerott, E.C.; Hayes, A.G.; Johnson, M.J.; Kirk, R.L.; McLennan, S.; Morris, R.V.; Scherr, L.M.; Schwochert, M.A.; Shiraishi, L.R.; Smith, G.H.; Soderblom, L.A.; Sohl-Dickstein, J. N.; Wadsworth, M.V.

    2003-01-01

    The Athena science payload on the Mars Exploration Rovers (MER) includes the Microscopic Imager (MI). The MI is a fixed-focus camera mounted on the end of an extendable instrument arm, the Instrument Deployment Device (IDD). The MI was designed to acquire images at a spatial resolution of 30 microns/pixel over a broad spectral range (400-700 nm). The MI uses the same electronics design as the other MER cameras but has optics that yield a field of view of 31 ?? 31 mm across a 1024 ?? 1024 pixel CCD image. The MI acquires images using only solar or skylight illumination of the target surface. A contact sensor is used to place the MI slightly closer to the target surface than its best focus distance (about 66 mm), allowing concave surfaces to be imaged in good focus. Coarse focusing (???2 mm precision) is achieved by moving the IDD away from a rock target after the contact sensor has been activated. The MI optics are protected from the Martian environment by a retractable dust cover. The dust cover includes a Kapton window that is tinted orange to restrict the spectral bandpass to 500-700 nm, allowing color information to be obtained by taking images with the dust cover open and closed. MI data will be used to place other MER instrument data in context and to aid in petrologic and geologic interpretations of rocks and soils on Mars. Copyright 2003 by the American Geophysical Union.

  1. Abrupt plate accelerations shape rifted continental margins.

    PubMed

    Brune, Sascha; Williams, Simon E; Butterworth, Nathaniel P; Müller, R Dietmar

    2016-08-11

    Rifted margins are formed by persistent stretching of continental lithosphere until breakup is achieved. It is well known that strain-rate-dependent processes control rift evolution, yet quantified extension histories of Earth's major passive margins have become available only recently. Here we investigate rift kinematics globally by applying a new geotectonic analysis technique to revised global plate reconstructions. We find that rifted margins feature an initial, slow rift phase (less than ten millimetres per year, full rate) and that an abrupt increase of plate divergence introduces a fast rift phase. Plate acceleration takes place before continental rupture and considerable margin area is created during each phase. We reproduce the rapid transition from slow to fast extension using analytical and numerical modelling with constant force boundary conditions. The extension models suggest that the two-phase velocity behaviour is caused by a rift-intrinsic strength--velocity feedback, which can be robustly inferred for diverse lithosphere configurations and rheologies. Our results explain differences between proximal and distal margin areas and demonstrate that abrupt plate acceleration during continental rifting is controlled by the nonlinear decay of the resistive rift strength force. This mechanism provides an explanation for several previously unexplained rapid absolute plate motion changes, offering new insights into the balance of plate driving forces through time.

  2. Obliquity along plate boundaries

    NASA Astrophysics Data System (ADS)

    Philippon, Mélody; Corti, Giacomo

    2016-12-01

    Most of the plate boundaries are activated obliquely with respect to the direction of far field stresses, as roughly only 8% of the plate boundaries total length shows a very low obliquity (ranging from 0 to 10°, sub-orthogonal to the plate displacement). The obliquity along plate boundaries is controlled by (i) lateral rheological variations within the lithosphere and (ii) consistency with the global plate circuit. Indeed, plate tectonics and magmatism drive rheological changes within the lithosphere and consequently influence strain localization. Geodynamical evolution controls large-scale mantle convection and plate formation, consumption, and re-organization, thus triggering plate kinematics variations, and the adjustment and re-orientation of far field stresses. These geological processes may thus result in plate boundaries that are not perpendicular but oblique to the direction of far field stresses. This paper reviews the global patterns of obliquity along plate boundaries. Using GPlate, we provide a statistical analysis of present-day obliquity along plate boundaries. Within this framework, by comparing natural examples and geological models, we discuss deformation patterns and kinematics recorded along oblique plate boundaries.

  3. Plating Tank Control Software

    SciTech Connect

    Krafcik, John

    1998-03-01

    The Plating Tank Control Software is a graphical user interface that controls and records plating process conditions for plating in high aspect ratio channels that require use of low current and long times. The software is written for a Pentium II PC with an 8 channel data acquisition card, and the necessary shunt resistors for measuring currents in the millampere range.

  4. Rotatable shear plate interferometer

    DOEpatents

    Duffus, Richard C.

    1988-01-01

    A rotatable shear plate interferometer comprises a transparent shear plate mounted obliquely in a tubular supporting member at 45.degree. with respect to its horizontal center axis. This tubular supporting member is supported rotatably around its center axis and a collimated laser beam is made incident on the shear plate along this center axis such that defocus in different directions can be easily measured.

  5. Cenozoic faults and faulting phases in the western Tarim Basin (NW China): Effects of the collisions on the southern margin of the Eurasian Plate

    NASA Astrophysics Data System (ADS)

    Li, Yue-Jun; Zhang, Qiang; Zhang, Guang-Ya; Tian, Zuo-Ji; Peng, Geng-Xin; Qiu, Bin; Huang, Zhi-Bin; Luo, Jun-Cheng; Wen, Lei; Zhao, Yan; Jia, Tie-Gan

    2016-12-01

    The Bachu Rise in the western Tarim Basin is the fore-bulge of the Kunlun late Cenozoic intra-continental foreland basin system formed under the far-field effect of India-Asia collision. Cenozoic faults and faulting are abnormally developed in the Bachu Rise and its adjacent area. Taking the Niaoshan-Gudongshan area on the southern boundary of the Bachu Rise as the key study area, 5 Cenozoic faulting phases were identified in the Bachu Rise and its adjacent area after careful seismic interpretation. They are end Cretaceous ∼ beginning Paleogene (ca. 65 Ma) décollement-thrusting, end Paleogene ∼ beginning Neogene (ca. 23 Ma) décollement-thrusting, end Miocene ∼ beginning Pliocene (ca. 5 Ma) basement-involved thrusting, late Pliocene ∼ early Pleistocene (ca. 3-2 Ma) normal faulting, middle Pleistocene ∼ Holocene (ca. <1.5 Ma) décollement-thrusting and strike-slip faulting. The Middle Cambrian and Paleogene gypsum-salt layers serve as the two main décollement layers in the study area. Thrusting of ca. 65 Ma was under the far-field effect of the collision between Lhasa (part of the Cimmerian Continent) and Asia; and the other 4 Cenozoic faulting phases were all under the far-field effect of the India-Asia collision. The late Cenozoic faulting is characterized by pulse thrust. There is one tectonic pause between each two successive thrust pulses. The compressive tectonic stress is weaker and even evolved into a slight tensional tectonic stress and forms normal fault in the tectonic pauses.

  6. Development of a new type of optical microscope

    NASA Astrophysics Data System (ADS)

    Luo, Ji-jun; Hou, Su-xia; Wang, Lian-feng; Sun, Hong-hui

    2007-12-01

    Along with the development and application of nanometer technology, a machine that allows deterministic position and manufacturing at the atomic level is required. An optical microscope of the machine was designed particularly to locate line features on a grid plate. It was designed to achieve nanometer level repeatability. The microscope uses a modulated light-emitting diode as its light source. It has two channels for optical imaging: one consists of two slits for precision measurement of line positions and the other of a camera for direct visual observation. A lock-in amplifier is used for demodulating the electrical signal from the photodiodes behind the slits. Initial experiments locating crosses on a grid plate indicate a repeatability of approximately 1 nm, one standard deviation. It was found that the measured line position is related not only to the focus but also to the illumination. There exists a particular orientation of the illumination where the measured line position is relatively insensitive to the focus, because of which the microscope is able to achieve nanometer level repeatability. Repeated measurements were performed on both an uncalibrated and a calibrated plate. Repeatabilities of better than 1 nm were obtained over time periods of several days; however, the ultimate accuracy of the machine still has to be demonstrated.

  7. Kinetics of phase separation and coarsening in dilute surfactant pentaethylene glycol monododecyl ether solutions.

    PubMed

    Tanaka, S; Kubo, Y; Yokoyama, Y; Toda, A; Taguchi, K; Kajioka, H

    2011-12-21

    We investigated the phase separation phenomena in dilute surfactant pentaethylene glycol monodedecyl ether (C(12)E(5)) solutions focusing on the growth law of separated domains. The solutions confined between two glass plates were found to exhibit the phase inversion, characteristic of the viscoelastic phase separation; the majority phase (water-rich phase) nucleated as droplets and the minority phase (micelle-rich phase) formed a network temporarily, then they collapsed into an usual sea-island pattern where minority phase formed islands. We found from the real-space microscopic imaging that the dynamic scaling hypothesis did not hold throughout the coarsening process. The power law growth of the domains with the exponent close to 1/3 was observed even though the coarsening was induced mainly by hydrodynamic flow, which was explained by Darcy's law of laminar flow.

  8. Malachite green photosensitive plates.

    PubMed

    Solano, C

    1989-08-15

    An experimental study of the behavior of malachite green sensitized plates was carried out. The transmittance variation of the irradiated plates was taken as a parameter. It has been observed that photoreduction in the malachite green plates is present only when ammonium dichromate is added to the plates. The introduction of external electron donors does not improve the photochemical reaction. It has been determined that malachite green molecules form a weak complex with the dichromate molecules and this complex can only be destroyed photochemically. This effect can explain the limited response of the malachite green dichromated plates.

  9. An improved plating process

    NASA Technical Reports Server (NTRS)

    Askew, John C.

    1994-01-01

    An alternative to the immersion process for the electrodeposition of chromium from aqueous solutions on the inside diameter (ID) of long tubes is described. The Vessel Plating Process eliminates the need for deep processing tanks, large volumes of solutions, and associated safety and environmental concerns. Vessel Plating allows the process to be monitored and controlled by computer thus increasing reliability, flexibility and quality. Elimination of the trivalent chromium accumulation normally associated with ID plating is intrinsic to the Vessel Plating Process. The construction and operation of a prototype Vessel Plating Facility with emphasis on materials of construction, engineered and operational safety and a unique system for rinse water recovery are described.

  10. The Latest in Handheld Microscopes

    ERIC Educational Resources Information Center

    Wighting, Mervyn J.; Lucking, Robert A.; Christmann, Edwin P.

    2004-01-01

    Around 1590, Zacharias Jansenn of Holland invented the microscope. Jansenn, an eyeglass maker by trade, experimented with lenses and discovered that things appeared closer with combinations of lenses. Over the past 400 years, several refinements to microscopes have occurred, making it possible to magnify objects between 200 and 1,500 times their…

  11. Scientists View Battery Under Microscope

    SciTech Connect

    2015-04-10

    PNNL researchers use a special microscope setup that shows the inside of a battery as it charges and discharges. This battery-watching microscope is located at EMSL, DOE's Environmental Molecular Sciences Laboratory that resides at PNNL. Researchers the world over can visit EMSL and use special instruments like this, many of which are the only one of their kind available to scientists.

  12. Scientists View Battery Under Microscope

    ScienceCinema

    None

    2016-07-12

    PNNL researchers use a special microscope setup that shows the inside of a battery as it charges and discharges. This battery-watching microscope is located at EMSL, DOE's Environmental Molecular Sciences Laboratory that resides at PNNL. Researchers the world over can visit EMSL and use special instruments like this, many of which are the only one of their kind available to scientists.

  13. High-throughput and sensitive quantitation of plasma catecholamines by ultraperformance liquid chromatography-tandem mass spectrometry using a solid phase microwell extraction plate.

    PubMed

    Dunand, Marielle; Gubian, Danilo; Stauffer, Maxime; Abid, Karim; Grouzmann, Eric

    2013-04-02

    Plasma catecholamines provide a reliable biomarker of sympathetic activity. The low circulating concentrations of catecholamines and analytical interferences require tedious sample preparation and long chromatographic runs to ensure their accurate quantification by HPLC with electrochemical detection. Published or commercially available methods relying on solid phase extraction technology lack sensitivity or require derivatization of catecholamine by hazardous reagents prior to tandem mass spectrometry (MS) analysis. Here, we manufactured a novel 96-well microplate device specifically designed to extract plasma catecholamines prior to their quantification by a new and highly sensitive ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Processing time, which included sample purification on activated aluminum oxide and elution, is less than 1 h per 96-well microplate. The UPLC-MS/MS analysis run time is 2.0 min per sample. This UPLC-MS/MS method does not require a derivatization step, reduces the turnaround time by 10-fold compared to conventional methods used for routine application, and allows catecholamine quantification in reduced plasma sample volumes (50-250 μL, e.g., from children and mice).

  14. Multicolor printing plate joining

    NASA Technical Reports Server (NTRS)

    Waters, W. J. (Inventor)

    1984-01-01

    An upper plate having ink flow channels and a lower plate having a multicolored pattern are joined. The joining is accomplished without clogging any ink flow paths. A pattern having different colored parts and apertures is formed in a lower plate. Ink flow channels each having respective ink input ports are formed in an upper plate. The ink flow channels are coated with solder mask and the bottom of the upper plate is then coated with solder. The upper and lower plates are pressed together at from 2 to 5 psi and heated to a temperature of from 295 F to 750 F or enough to melt the solder. After the plates have cooled and the pressure is released, the solder mask is removed from the interior passageways by means of a liquid solvent.

  15. A STED-FLIM microscope applied to imaging the natural killer cell immune synapse

    NASA Astrophysics Data System (ADS)

    Lenz, M. O.; Brown, A. C. N.; Auksorius, E.; Davis, D. M.; Dunsby, C.; Neil, M. A. A.; French, P. M. W.

    2011-03-01

    We present a stimulated emission depletion (STED) fluorescence lifetime imaging (FLIM) microscope, excited by a microstructured optical fibre supercontinuum source that is pumped by a femtosecond Ti:Sapphire-laser, which is also used for depletion. Implemented using a piezo-scanning stage on a laser scanning confocal fluorescence microscope system with FLIM realised using time correlated single photon counting (TCSPC), this provides convenient switching between confocal and STED-FLIM with spatial resolution down to below 60 nm. We will present our design considerations to make a robust instrument for biological applications including a comparison between fixed phase plate and spatial light modulator (SLM) approaches to shape the STED beam and the correlation of STED and confocal FLIM microscopy. Following our previous application of FLIM-FRET to study intercellular signalling at the immunological synapse (IS), we are employing STED microscopy to characterize the spatial distribution of cellular molecules with subdiffraction resolution at the IS. In particular, we are imaging cytoskeletal structure at the Natural Killer cell activated immune synapse. We will also present our progress towards multilabel STED microscopy to determine how relative spatial molecular organization, previously undetectable by conventional microscopy techniques, is important for NK cell cytotoxic function. Keywords: STED, Stimulated Emission Depletion Microscopy, Natural Killer (NK) cell, Fluorescence lifetime imaging, FLIM, Super resolution microscopy.

  16. The head-mounted microscope.

    PubMed

    Chen, Ting; Dailey, Seth H; Naze, Sawyer A; Jiang, Jack J

    2012-04-01

    Microsurgical equipment has greatly advanced since the inception of the microscope into the operating room. These advancements have allowed for superior surgical precision and better post-operative results. This study focuses on the use of the Leica HM500 head-mounted microscope for the operating phonosurgeon. The head-mounted microscope has an optical zoom from 2× to 9× and provides a working distance from 300 mm to 700 mm. The headpiece, with its articulated eyepieces, adjusts easily to head shape and circumference, and offers a focus function, which is either automatic or manually controlled. We performed five microlaryngoscopic operations utilizing the head-mounted microscope with successful results. By creating a more ergonomically favorable operating posture, a surgeon may be able to obtain greater precision and success in phonomicrosurgery. Phonomicrosurgery requires the precise manipulation of long-handled cantilevered instruments through the narrow bore of a laryngoscope. The head-mounted microscope shortens the working distance compared with a stand microscope, thereby increasing arm stability, which may improve surgical precision. Also, the head-mounted design permits flexibility in head position, enabling operator comfort, and delaying musculoskeletal fatigue. A head-mounted microscope decreases the working distance and provides better ergonomics in laryngoscopic microsurgery. These advances provide the potential to promote precision in phonomicrosurgery.

  17. Force-plate analyses of balance following a balance exercise program during acute post-operative phase in individuals with total hip and knee arthroplasty: A randomized clinical trial

    PubMed Central

    Jogi, Pankaj; Zecevic, Aleksandra; Overend, Tom J; Spaulding, Sandi J; Kramer, John F

    2016-01-01

    Objectives: Typical rehabilitation programs following total hip arthroplasty and total knee arthroplasty include joint range of motion and muscle-strengthening exercises. Balance and balance exercises following total hip arthroplasty and total knee arthroplasty have not received much attention. The purpose of this study was to determine whether an intervention of balance exercises added to a typical rehabilitation program positively affects patients’ balance. Methods: A total of 63 patients were provided with outpatient physical therapy at their home. Patients were randomly assigned to either typical (n = 33) or balance (n = 30) exercise group. The typical group completed seven typical surgery-specific joint range of motion and muscle-strengthening exercises, while the balance group completed the typical exercises plus three balance exercises. After 5 weeks of administering the rehabilitation program, patients’ balance was assessed on a force plate using 95% ellipse area of the center of pressure amplitude. Results: Patients in the balance group demonstrated significant reduction in the 95% ellipse area for the anterior and posterior lean standing conditions (p < 0.01). Conclusion: Balance exercises added to the typical outpatient physical therapy program resulted in significantly greater improvements in balance for participants with total hip arthroplasty or total knee arthroplasty, compared to the typical exercise program alone. Physical therapists might consider the use of balance exercises to improve balance in individuals in the acute post-operative phase following total hip arthroplasty or total knee arthroplasty. PMID:27867519

  18. Large-Area Zone Plate Fabrication with Optical Lithography

    SciTech Connect

    Denbeaux, G.

    2011-09-09

    Zone plates as condenser optics for x-ray microscopes offer simple optical designs for both illumination and spectral resolution when used as a linear monochromator. However, due to the long write times for electron beam lithography, both the availability and the size of zone plates for condensers have been limited. Since the resolution provided by the linear monochromator scales almost linearly with the diameter of the zone plate, the full potential for zone plate monochromators as illumination systems for x-ray microscopes has not been achieved. For example, the 10-mm-diameter zone plate has demonstrated a spectral resolution of E/{Delta}E = 700[1], but with a 26-mm-diameter zone plate, the calculated spectral resolution is higher than E/{Delta}E = 3000. These large-area zone plates are possible to fabricate with the leading edge semiconductor lithography tools such as those available at the College of Nanoscale Science and Engineering at the University at Albany. One of the lithography tools available is the ASML TWINSCAN XT: 1950i with 37-nm resolution [2]. A single 300-mm wafer can contain more than 60 fields, each with a large area condenser, and the throughput of the tool can be more than one wafer every minute.

  19. GOLD PLATING PROCESS

    DOEpatents

    Seegmiller, R.

    1957-08-01

    An improved bath is reported for plating gold on other metals. The composition of the plating bath is as follows: Gold cyanide from about 15 to about 50 grams, potassium cyanide from about 70 to about 125 grams, and sulfonated castor oil from about 0.1 to about 10 cc. The gold plate produced from this bath is smooth, semi-hard, and nonporous.

  20. Plating methods, a survey

    NASA Technical Reports Server (NTRS)

    Berkowitz, J. B.; Emerson, N. H.

    1972-01-01

    Results are presented of a comprehensive search of the literature available, much of which has been generated by the research centers of NASA and its contractors, on plating and coating methods and techniques. Methods covered included: (1) electroplating from aqueous solutions; (2) electroplating from nonaqueous solutions; (3) electroplating from fused-salt baths; (4) electroforming; (5) electroless plating, immersion plating, and mirroring; (6) electroplating from gaseous plasmas; and (7) anodized films and conversion coatings.

  1. Adirondack Under the Microscope-2

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This overhead look at the martian rock dubbed Adirondack was captured by the Mars Exploration Rover Spirit's panoramic camera. It shows the approximate region where the rover's microscopic imager began its first close-up inspection.

  2. Magnetic Resonance Force Microscope Development

    SciTech Connect

    Hammel, P.C.; Zhang, Z.; Suh, B.J.; Roukes, M.L.; Midzor, M.; Wigen, P.E.; Childress, J.R.

    1999-06-03

    Our objectives were to develop the Magnetic Resonance Force Microscope (MRFM) into an instrument capable of scientific studies of buried structures in technologically and scientifically important electronic materials such as magnetic multilayer materials. This work resulted in the successful demonstration of MRFM-detected ferromagnetic resonance (FMR) as a microscopic characterization tool for thin magnetic films. Strong FMR spectra obtained from microscopic Co thin films (500 and 1000 angstroms thick and 40 x 200 microns in lateral extent) allowed us to observe variations in sample inhomogeneity and magnetic anisotropy field. We demonstrated lateral imaging in microscopic FMR for the first time using a novel approach employing a spatially selective local field generated by a small magnetically polarized spherical crystallite of yttrium iron garnet. These successful applications of the MRFM in materials studies provided the basis for our successful proposal to DOE/BES to employ the MRF M in studies of buried interfaces in magnetic materials.

  3. Microscopic derivation of discrete hydrodynamics.

    PubMed

    Español, Pep; Anero, Jesús G; Zúñiga, Ignacio

    2009-12-28

    By using the standard theory of coarse graining based on Zwanzig's projection operator, we derive the dynamic equations for discrete hydrodynamic variables. These hydrodynamic variables are defined in terms of the Delaunay triangulation. The resulting microscopically derived equations can be understood, a posteriori, as a discretization on an arbitrary irregular grid of the Navier-Stokes equations. The microscopic derivation provides a set of discrete equations that exactly conserves mass, momentum, and energy and the dissipative part of the dynamics produces strict entropy increase. In addition, the microscopic derivation provides a practical implementation of thermal fluctuations in a way that the fluctuation-dissipation theorem is satisfied exactly. This paper points toward a close connection between coarse-graining procedures from microscopic dynamics and discretization schemes for partial differential equations.

  4. Microscopic Procedures for Plant Meiosis.

    ERIC Educational Resources Information Center

    Braselton, James P.

    1997-01-01

    Describes laboratory techniques designed to familiarize students with meiosis and how microscopic preparations of meiosis are made. These techniques require the use of fresh or fixed flowers. Contains 18 references. (DDR)

  5. Recent Athena Microscopic Imager Results

    NASA Astrophysics Data System (ADS)

    Herkenhoff, K. E.; Ashley, J. W.; Johnson, J. R.; Parker, T. J.; Athena Science Team

    2012-03-01

    The Mars Exploration Rover Opportunity arrived at the rim of Endeavour Crater in August 2011. This presentation summarizes Opportunity Microscopic Imager observations of ejecta, bedrock, a gypsum vein, and other materials in the crater rim rocks.

  6. (Center of excellence: Microlaser microscope)

    SciTech Connect

    Webb, R.H.

    1992-01-01

    This Center-of-Excellence grant has two components: development of an imaging system based on microlaser arrays forms a central project among a group of laser diagnostic and therapeutic efforts primarily funded outside the grant. In these first 8 months we have set up the Microlaser Microscope using small microlaser arrays. We have emphasized the basics of microlaser handling and electronic addressing and the optics of the microscope. Details of electronics and optics given here will be used in the larger arrays which should be available soon. After a description of the central Microlaser Microscope project, we touch briefly on the other projects of the Center, which have been outstandingly fruitful this year. Publications are necessarily concerned with the smaller projects, since the Microlaser Microscope is in its early stages.

  7. A portable laser photostimulation and imaging microscope.

    PubMed

    Nikolenko, Volodymyr; Peterka, Darcy S; Yuste, Rafael

    2010-08-01

    We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light. The flexibility of SLMs, which can mimic virtually any optical transfer function, enables the experimenter to create, in software, arbitrary spatio-temporal light patterns, including focusing and beam scanning, simply by calculating the appropriate phase mask. Our prototype, a scan-less device with no moving parts, can be used for laser imaging or photostimulation, supplanting the need for an elaborate optical setup. As a proof of principle, we generate complex excitation patterns on fluorescent samples and also perform functional imaging of neuronal activity in living brain slices.

  8. PLATES WITH OXIDE INSERTS

    DOEpatents

    West, J.M.; Schumar, J.F.

    1958-06-10

    Planar-type fuel assemblies for nuclear reactors are described, particularly those comprising fuel in the oxide form such as thoria and urania. The fuel assembly consists of a plurality of parallel spaced fuel plate mennbers having their longitudinal side edges attached to two parallel supporting side plates, thereby providing coolant flow channels between the opposite faces of adjacent fuel plates. The fuel plates are comprised of a plurality of longitudinally extending tubular sections connected by web portions, the tubular sections being filled with a plurality of pellets of the fuel material and the pellets being thermally bonded to the inside of the tubular section by lead.

  9. CALUTRON FACE PLATE

    DOEpatents

    Brobeck, W.M.

    1959-08-25

    The construction of a removable cover plate for a calutron tank is described. The plate is fabricated of a rectangular frame member to which is welded a bowed or dished plate of thin steel, reinforced with transverse stiffening ribs. When the tank is placed between the poles of a magnet, the plate may be pivoted away from the tank and magnet and is adapted to support the ion separation mechanism secured to its inner side as well as the vacuum load within the tank.

  10. Electroless Nickel Phosphorus Plating on AZ31

    NASA Astrophysics Data System (ADS)

    Shartal, Kh. M.; Kipouros, G. J.

    2009-04-01

    One of the major drawbacks to using magnesium parts in automotive applications is poor corrosion resistance, which can be improved with a nickel-boron coating placed on a nickel-phosphorus coating, which, in turn, is placed on a phosphate-permanganate conversion-coating layer produced on the magnesium alloy AZ31. This work reports on the determination of the optimum kinetic parameters for producing a coherent nickel-phosphorus coating using an electroless-procedure phosphate-permanganate conversion-coating layer and for studying the effects of the experimental variables of the electroless plating process on the phosphorus content, surface morphology, and structure of the electroless nickel-phosphorus (EN-P) coatings produced. Measurements of the plating rate as a function of experimental variables such as the compositions of the plating bath constituents, temperature, and pH were implemented using the weight-gain method; the phosphorus content of the EN-P coatings was measured using energy-dispersive spectroscopy (EDS) analysis. The surface morphology of the coating was examined using a scanning electron microscope (SEM); X-ray diffraction (XRD) was used to characterize the structure of each coating. An empirical rate law was determined for EN-P plating on a phosphate-permanganate conversion coating. It is found that the deposition rate of the EN-P coating increases by increasing the deposition temperature, the concentration of free nickel ions, and the concentration of hypophosphite ions in the plating bath. In addition, the deposition rate decreases by increasing both the plating bath pH and the concentration of citric acid in the plating bath.

  11. HIGH TEMPERATURE MICROSCOPE AND FURNACE

    DOEpatents

    Olson, D.M.

    1961-01-31

    A high-temperature microscope is offered. It has a reflecting optic situated above a molten specimen in a furnace and reflecting the image of the same downward through an inert optic member in the floor of the furnace, a plurality of spaced reflecting plane mirrors defining a reflecting path around the furnace, a standard microscope supported in the path of and forming the end terminus of the light path.

  12. Fluorescence Talbot microscope using incoherent source

    NASA Astrophysics Data System (ADS)

    Sun, Yangyang; Pang, Shuo

    2016-08-01

    Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15 mm×15 mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence of the illumination source, limiting their potential applications in low-resource setting environment. A more cost-effective setup using a light-emitting diode, which has an area of 4 mm2 and a full width at half maximum of 16 nm in wavelength, is demonstrated. Compared to the illumination that is spatially filtered by a single pinhole, our system has achieved an illumination intensity that is 357 times higher. The reconstructed image quality is comparable to that of a 10× microscope objective. Various samples, such as fluorescent beads, green fluorescence protein-labeled HeLa cells, and a mouse kidney slide, were reconstructed by the system.

  13. STM-SQUID probe microscope

    NASA Astrophysics Data System (ADS)

    Hayashi, Tadayuki; Tachiki, Minoru; Itozaki, Hideo

    2007-11-01

    We have developed a STM-SQUID probe microscope. A high TC SQUID probe microscope was combined with a scanning tunneling microscope for investigation of samples at room temperature in air. A high permeability probe needle was used as a magnetic flux guide to improve the spatial resolution. The probe with tip radius of less than 100 nm was prepared by microelectropolishing. The probe was also used as a scanning tunneling microscope tip. Topography of the sample surface could be measured by the scanning tunneling microscope with high spatial resolution prior to observation by SQUID microscopy. The SQUID probe microscope image could be observed while keeping the distance from the sample surface to the probe tip constant. We observed a topographic image and a magnetic image of Ni fine pattern and also a magnetically recorded hard disk. Furthermore we have investigated a sample vibration method of the static magnetic field emanating from a sample with the aim of achieving a higher signal-to-noise (S/N) ratio.

  14. Single-wavelength STED microscope

    NASA Astrophysics Data System (ADS)

    Baer, Stephen C.

    2011-03-01

    The zero-point STED microscope (US Pat. 5,866,911)1 was the first far-field microscope to overcome the diffraction limit, but optimally it requires two expensive synchronized short-pulsed lasers. Replacing the synchronized pulsed lasers with CW lasers had been proposed to reduce costs1, but this seriously reduced resolution compared to a similarly powered pulsed STED microscope. A recent theoretical and experimental study (Nat. Methods 4, 915 (2007))3 argued that CW STED has better resolution than previously believed, but there appear to be flaws in the theory sufficient to raise questions about its reported experimental confirmation. We describe an alternative approach to reducing cost of the STED microscope while preserving resolution. A portion of the beam from a femtosecond pulsed laser of a wavelength able to excite fluorescence by multiphoton absorption, is passed through a long optical fiber to stretch the pulses to reduce their peak power so they can no longer excite but can quench by stimulated emission. The stretched pulses are shaped into a doughnut profile and then recombined with the first beam for interaction with the specimen. With suitable fluorophores, this instrument should be able to match the resolution performance of the pulsed laser STED microscope using separate lasers. Particularly when added to an existing multiphoton microscope, such performance should be achievable at extremely low added cost.

  15. Gold nanowires fabricated by immersion plating.

    PubMed

    Hsu, Chih-Chieh; Shen, Fang-Yee; Huang, Fon-Shan

    2008-05-14

    The growth mechanism of oriented Au nanowires fabricated by immersion plating was investigated. Both n-type crystal Si (c-Si) and amorphous Si (a-Si) with an electron-beam (E-beam) patterned resist nanotrench were immersed into the plating bath HAuCl(4)/HF. For the Au nanowires fabricated on c-Si, voids, nanograins, and clusters were observed at various plating conditions, time and temperature. The voids were often found in the center of the Au nanowires due to there being fewer nucleation sites on the c-Si surface. However, Au can easily nucleate on the surface of a-Si and form continuous Au nanowires with grain sizes about 10-50 nm. The resistivities of Au nanowires with width 105 nm fabricated on a-Si are about 4.4-6.5 µΩ cm. After annealing at 200 °C for 30 min in N(2) ambient, the resistivities are lowered to about 3.0-3.9 µΩ cm, measured in an atomic force microscope (AFM) in contact mode. The grain size of Au is in the range of ∼50-100 nm. A scanning electron microscope (SEM) examination and grazing incident x-ray diffraction (GIXRD) analysis were also carried out to study the morphology and crystalline structure of the Au nanowires.

  16. Development of flat - plate solar plate collector - evaporator. Summary report

    SciTech Connect

    Abramzon, B.; Yaron, I.

    1981-11-01

    In the present study the thermal performance of a flat plate solar collector is analyzed theoretically for the case in which the working fluid may undergo a phase change within the tubes of the collector. In addition to the common domestic applications, such a collector - evaporator may be used as a generator of vapors for the production of mechanical or electrical energy, e.g. solar water pumps, solar power stations, etc., as well as for solar - powered absorption refrigeration machines, distillation installations, etc.

  17. A mini-microscope for in situ monitoring of cells.

    PubMed

    Kim, Sang Bok; Koo, Kyo-in; Bae, Hojae; Dokmeci, Mehmet R; Hamilton, Geraldine A; Bahinski, Anthony; Kim, Sun Min; Ingber, Donald E; Khademhosseini, Ali

    2012-10-21

    A mini-microscope was developed for in situ monitoring of cells by modifying off-the-shelf components of a commercial webcam. The mini-microscope consists of a CMOS imaging module, a small plastic lens and a white LED illumination source. The CMOS imaging module was connected to a laptop computer through a USB port for image acquisition and analysis. Due to its compact size, 8 × 10 × 9 cm, the present microscope is portable and can easily fit inside a conventional incubator, and enables real-time monitoring of cellular behaviour. Moreover, the mini-microscope can be used for imaging cells in conventional cell culture flasks, such as Petri dishes and multi-well plates. To demonstrate the operation of the mini-microscope, we monitored the cellular migration of mouse 3T3 fibroblasts in a scratch assay in medium containing three different concentrations of fetal bovine serum (5, 10, and 20%) and demonstrated differential responses depending on serum levels. In addition, we seeded embryonic stem cells inside poly(ethylene glycol) microwells and monitored the formation of stem cell aggregates in real time using the mini-microscope. Furthermore, we also combined a lab-on-a-chip microfluidic device for microdroplet generation and analysis with the mini-microscope and observed the formation of droplets under different flow conditions. Given its cost effectiveness, robust imaging and portability, the presented platform may be useful for a range of applications for real-time cellular imaging using lab-on-a-chip devices at low cost.

  18. Development of grating-based x-ray phase tomography under the ERATO project

    NASA Astrophysics Data System (ADS)

    Momose, Atsushi; Takano, Hidekazu; Hoshino, Masato; Yashiro, Wataru; Wu, Yanlin

    2016-10-01

    We have launched a project to promote grating-based X-ray phase imaging/tomography extensively. Here, two main activities are presented for enabling dynamic, or four-dimensional, X-ray phase tomography and nanoscopic X-ray phase tomography by grating interferometry. For the former, while some demonstrations in this direction were performed with white synchrotron radiation, improvement in image quality by spectrum tuning is described. A preliminary result by a total reflection mirror is presented, and as a next step, preparation of a 10% bandpass filter by a multilayer mirror is reported. For the latter, X-ray microscopes available both at synchrotron radiation facilities and laboratories equipped with a Fresnel zone plate are combined with grating interferometry. Here, a preliminary result with a combination of a Lau interferometer and a laboratory-based X-ray microscope is presented.

  19. Characterization of Al-Cu-Mg-Ag Alloy RX226-T8 Plate

    NASA Technical Reports Server (NTRS)

    Lach, Cynthia L.; Domack, Marcia S.

    2003-01-01

    Aluminum-copper-magnesium-silver (Al-Cu-Mg-Ag) alloys that were developed for thermal stability also offer attractive ambient temperature strength-toughness combinations, and therefore, can be considered for a broad range of airframe structural applications. The current study evaluated Al-Cu-Mg-Ag alloy RX226-T8 in plate gages and compared performance with sheet gage alloys of similar composition. Uniaxial tensile properties, plane strain initiation fracture toughness, and plane stress tearing resistance of RX226-T8 were examined at ambient temperature as a function of orientation and thickness location in the plate. Properties were measured near the surface and at the mid-plane of the plate. Tensile strengths were essentially isotropic, with variations in yield and ultimate tensile strengths of less than 2% as a function of orientation and through-thickness location. However, ductility varied by more than 15% with orientation. Fracture toughness was generally higher at the mid-plane and greater for the L-T orientation, although the differences were small near the surface of the plate. Metallurgical analysis indicated that the microstructure was primarily recrystallized with weak texture and was uniform through the plate with the exception of a fine-grained layer near the surface of the plate. Scanning electron microscope analysis revealed Al-Cu-Mg second phase particles which varied in composition and were primarily located on grain boundaries parallel to the rolling direction. Fractography of toughness specimens for both plate locations and orientations revealed that fracture occurred predominantly by transgranular microvoid coalescence. Introduction High-strength, low-density Al-Cu-Mg-Ag alloys were initially developed to replace conventional 2000 (Al-Cu-Mg) and 7000 (Al-Zn-Cu-Mg) series aluminum alloys for aircraft structural applications [1]. During the High Speed Civil Transport (HSCT) program, improvements in thermal stability were demonstrated for candidate

  20. Scanning Miniature Microscopes without Lenses

    NASA Technical Reports Server (NTRS)

    Wang, Yu

    2009-01-01

    The figure schematically depicts some alternative designs of proposed compact, lightweight optoelectronic microscopes that would contain no lenses and would generate magnified video images of specimens. Microscopes of this type were described previously in Miniature Microscope Without Lenses (NPO - 20218), NASA Tech Briefs, Vol. 22, No. 8 (August 1998), page 43 and Reflective Variants of Miniature Microscope Without Lenses (NPO 20610), NASA Tech Briefs, Vol. 26, No. 9 (September 1999), page 6a. To recapitulate: In the design and construction of a microscope of this type, the focusing optics of a conventional microscope are replaced by a combination of a microchannel filter and a charge-coupled-device (CCD) image detector. Elimination of focusing optics reduces the size and weight of the instrument and eliminates the need for the time-consuming focusing operation. The microscopes described in the cited prior articles contained two-dimensional CCDs registered with two-dimensional arrays of microchannels and, as such, were designed to produce full two-dimensional images, without need for scanning. The microscopes of the present proposal would contain one-dimensional (line image) CCDs registered with linear arrays of microchannels. In the operation of such a microscope, one would scan a specimen along a line perpendicular to the array axis (in other words, one would scan in pushbroom fashion). One could then synthesize a full two-dimensional image of the specimen from the line-image data acquired at one-pixel increments of position along the scan. In one of the proposed microscopes, a beam of unpolarized light for illuminating the specimen would enter from the side. This light would be reflected down onto the specimen by a nonpolarizing beam splitter attached to the microchannels at their lower ends. A portion of the light incident on the specimen would be reflected upward, through the beam splitter and along the microchannels, to form an image on the CCD. If the

  1. Blue Willow Story Plates

    ERIC Educational Resources Information Center

    Fontes, Kris

    2009-01-01

    In the December 1997 issue of "SchoolArts" is a lesson titled "Blue Willow Story Plates" by Susan Striker. In this article, the author shares how she used this lesson with her middle-school students many times over the years. Here, she describes a Blue Willow plate painting project that her students made.

  2. Earthquakes and plate tectonics

    USGS Publications Warehouse

    Spall, H.

    1977-01-01

    An explanation is to be found in plate tectonics, a concept which has revolutionized thinking in the Earth sciences in the last 10 years. The theory of plate tectonics combines many of the ideas about continental drift (originally proposed in 1912 by Alfred Wegener in Germany) and sea-floor spreading (suggested originally by Harry Hess of Princeton University). 

  3. A modified agar plate method for detection of Strongyloides stercoralis.

    PubMed

    Koga, K; Kasuya, S; Khamboonruang, C; Sukhavat, K; Ieda, M; Takatsuka, N; Kita, K; Ohtomo, H

    1991-10-01

    The agar plate method is a new technique with high detection rates for coprological diagnosis of human strongyloidiasis. This report details modifications of the technique and establishes a standardized procedure. We recommend that all plates should be carefully observed using a microscope because macroscopic observation can lead to false negative results. It is also advisable to pour formalin solution directly into microscopically positive dishes to collect worms by sedimentation. This procedure enables one to observe worms otherwise hidden. Sealing dishes with adhesive tape prevents larvae from crawling out of the dishes, eliminating any possibility in the reduction of detection rates, and greatly improves the safety conditions for the technician performing the procedure. We consider the agar plate method to be superior to the filter paper method in detecting Strongyloides, and we believe that it will eventually become the technique of choice.

  4. Turbine vane plate assembly

    DOEpatents

    Schiavo Jr., Anthony L.

    2006-01-10

    A turbine vane assembly includes a turbine vane having first and second shrouds with an elongated airfoil extending between. Each end of the airfoil transitions into a shroud at a respective junction. Each of the shrouds has a plurality of cooling passages, and the airfoil has a plurality of cooling passages extending between the first and second shrouds. A substantially flat inner plate and an outer plate are coupled to each of the first and second shrouds so as to form inner and outer plenums. Each inner plenum is defined between at least the junction and the substantially flat inner plate; each outer plenum is defined between at least the substantially flat inner plate and the outer plate. Each inner plenum is in fluid communication with a respective outer plenum through at least one of the cooling passages in the respective shroud.

  5. Performance of hard X-ray zone plates at the Advanced Photon Source

    SciTech Connect

    Maser, J.; Lai, B.; Cai, Z.; Rodrigues, W.; Legnini, D.; Ilinski, P.; Yun, W.; Chen, Z.; Krasnoperova, A.A.; Vladimirsky, Y.; Cerrina, F.; Di, E.; Fabrizio, E.; Gentili, M.

    1999-12-20

    Fresnel zone plates have been highly successful as focusing and imaging optics for soft x-ray microscopes and microprobe. More recently, with the advent of third-generation high-energy storage rings, zone plates for the hard x-ray regime have been put to use as well. The performance of zone plates manufactured using a combination of electron-beam lithography and x-ray lithography is described.

  6. Soft X-ray microscope with nanometer spatial resolution and its applications

    NASA Astrophysics Data System (ADS)

    Wachulak, P. W.; Torrisi, A.; Bartnik, A.; Wegrzynski, L.; Fok, T.; Patron, Z.; Fiedorowicz, H.

    2016-12-01

    A compact size microscope based on nitrogen double stream gas puff target soft X-ray source, which emits radiation in water-window spectral range at the wavelength of λ = 2.88 nm is presented. The microscope employs ellipsoidal grazing incidence condenser mirror for sample illumination and silicon nitride Fresnel zone plate objective for object magnification and imaging. The microscope is capable of capturing water-window images of objects with 60 nm spatial resolution and exposure time as low as a few seconds. Details about the microscopy system as well as some examples of different applications from various fields of science, are presented and discussed.

  7. Thermal Casimir interaction between two magnetodielectric plates

    SciTech Connect

    Geyer, B.; Klimchitskaya, G. L.; Mostepanenko, V. M.

    2010-03-01

    We investigate the thermal Casimir interaction between two magnetodielectric plates made of real materials. On the basis of the Lifshitz theory, it is shown that for diamagnets and for paramagnets in the broad sense (with exception of ferromagnets) the magnetic properties do not influence the magnitude of the Casimir force. For ferromagnets, taking into account the realistic dependence of magnetic permeability on frequency, we conclude that the impact of magnetic properties on the Casimir interaction arises entirely from the contribution of the zero-frequency term in the Lifshitz formula. The computations of the Casimir free energy and pressure are performed for the configurations of two plates made of ferromagnetic metals (Co and Fe), for one plate made of ferromagnetic metal and the other of nonmagnetic metal (Au), for two ferromagnetic dielectric plates (on the basis of polystyrene), and for a ferromagnetic dielectric plate near a nonmagnetic metal plate. The dielectric permittivity of metals is described using both the Drude and the plasma model approaches. It is shown that the Casimir repulsion through the vacuum gap can be realized in the configuration of a ferromagnetic dielectric plate near a nonmagnetic metal plate described by the plasma model. In all cases considered, the respective analytical results in the asymptotic limit of large separations between the plates are obtained. The impact of the magnetic phase transition through the Curie temperature on the Casimir interaction is considered. In conclusion, we propose several experiments allowing to determine whether the magnetic properties really influence the Casimir interaction and to independently verify the Drude and plasma model approaches to the thermal Casimir force.

  8. Comparison of assessment of pigeon sperm viability by contrast-phase microscope (eosin-nigrosin staining) and flow cytometry (SYBR-14/propidium iodide (PI) staining) [evaluation of pigeon sperm viability].

    PubMed

    Klimowicz-Bodys, M D; Batkowski, F; Ochrem, A S; Savič, M A

    2012-02-01

    The aim of these experiments was to compare the conventional, microscopic method of evaluating pigeon sperm viability to sperm assessed by flow cytometry. Semen was collected twice a week from two groups of pigeons. In every group were 20 males (Group I: meat-type breed; Group II: fancy pigeon breed). Semen was collected using the lumbosacral and cloacal region massage method. Ejaculates collected from each group were pooled and diluted to 10 × 10(6) sperm/ml in BPSE solution. Samples were divided into three equal parts and estimated after collection as well as after in vitro storage for 3, 6 and 24 h. The first part was using for semen motility evaluation. The proportion of motile spermatozoa (MOT) and progressive movement (PMOT) of fresh and stored semen were evaluated using the CASA-system. The second part was examined subjectively by microscope (eosin-nigrosin (EN), eosin-nigrosin staining), the third one was assessed using dual fluorescence SYBR-14/propidium iodide (PI) and flow cytometry (FC). There were not any significant differences in sperm viability and motility between the groups at 0, 3, 6, and 24 h post collection. The percentage of viable spermatozoa in fresh semen determined by EN and FC was not different in Groups I and II (I - 88.71 ± 5.42 and 84.01 ± 3.19, respectively; II-90.87 ± 6.01 and 87.38 ± 5.57, respectively). Significantly lower percentages of viable spermatozoa were detected by FC compared to the EN method in both groups after 6 h (P ≤ 0.05) as well as 24 h (P ≤ 0.01) of storage. Moreover, the dual fluorescent SYBR-14/PI staining allowed for the identification a third population of double stained, moribund spermatozoa. High positive correlations in percentage of live spermatozoa were noted between EN and FC methods in both groups of birds. Evaluation of sperm viability by FC is a rapid, accurate, sensitive, and objective method for the assessment of pigeon sperm viability in fresh as well as stored semen.

  9. Scanning thermal-conductivity microscope

    NASA Astrophysics Data System (ADS)

    Sarid, Dror; McCarthy, Brendan; Grover, Ranjan

    2006-02-01

    This article describes a novel implementation of an atomic force microscope that can map thermal-conductivity features across a sample with a high spatial resolution. The microscope employs a single-sided, metal-coated cantilever, which acts as a bimetallic strip together with a heating laser whose beam is focused on the cantilever's free end, on the opposite side of its tip. Subtracting the topography obtained by the unheated and heated cantilevers yields a map of thermal conductivity across the surface of a sample. The article presents (a) the theory of operation of the microscope and (b) the experimental results obtained on a silicon sample with oxide features, showing good agreement between the two.

  10. Mosaic of Commemorative Microscope Substrate

    NASA Technical Reports Server (NTRS)

    2008-01-01

    Written by electron beam lithography in the Microdevices Laboratory of NASA's Jet Propulsion Laboratory, this Optical Microscope substrate helps the Phoenix Mars Mission science team learn how to assemble individual microscope images into a mosaic by aligning rows of text.

    Each line is about 0.1 millimeter tall, the average thickness of a human hair. Except for the Mogensen twins, the names are of babies born and team members lost during the original development of MECA (the Microscopy, Electrochemistry and Conductivity Analyzer) for the canceled 2001 Mars lander mission. The plaque also acknowledges the MECA 2001 principal investigator, now retired.

    This image was taken by the MECA Optical Microscope on Sol 111, or the 111th day of the Phoenix mission (Sept. 16, 2008).

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by JPL, Pasadena, Calif. Spacecraft development was by Lockheed Martin Space Systems, Denver.

  11. Microscopic characterization of peptide nanostructures.

    PubMed

    Mammadov, Rashad; Tekinay, Ayse B; Dana, Aykutlu; Guler, Mustafa O

    2012-02-01

    Peptide-based nanomaterials have been utilized for various applications from regenerative medicine to electronics since they provide several advantages including easy synthesis methods, numerous routes for functionalization and biomimicry of secondary structures of proteins which leads to design of self-assembling peptide molecules to form nanostructures. Microscopic characterization at nanoscale is critical to understand processes directing peptide molecules to self-assemble and identify structure-function relationship of the nanostructures. Here, fundamental studies in microscopic characterization of peptide nanostructures are discussed to provide insights in widely used microscopy tools. In this review, we will encompass characterization studies of peptide nanostructures with modern microscopes, such as TEM, SEM, AFM, and advanced optical microscopy techniques. We will also mention specimen preparation methods and describe interpretation of the images.

  12. Plate Boundary Observatory in Taiwan

    NASA Astrophysics Data System (ADS)

    Yu, S.; Tsai, C.

    2003-12-01

    The island of Taiwan is situated in the plate boundary zone between the Eurasian and the Philippine Sea plates. The Philippine Sea plate is subducting northwestward underneath the Eurasian plate along the Ryukyu Trench in the north, while the Eurasian plate underthrusts the Philippine Sea plate along the Manila Trench in the south. Taking advantage of the extremely high strain rate in the Taiwan area, an integrated National Science Council project, Plate Boundary Observatory in Taiwan (PBOT), was initiated following the idea of US PBO. The scientific goal of PBOT is to observe the crustal deformation on various temporal and spatial scales in the Taiwan plate boundary zone employing available state of the art techniques for measuring crustal strain. The techniques include seismology, Global Positioning System (GPS), Interferometric Synthetic Aperture Radar (InSAR), borehole strainmeter, and earthquake geology. They are complementary to each other and form a complete spectrum of measuring various periods of crustal strain. The process of crustal deformation is generally quite slow. To obtain a reliable result, we usually need to persist in the observations for several years or even decades. Thus the PBOT should be a long-term project. In the first phase of 3 years period from 2003 to 2006, we will focus on the two areas, i.e. the plate suture zone in the Longitudinal Valley area and the western Taiwan where the higher seismic hazard is expected. A five-year national program, entitled ­Program for Earthquake and Active-fault Research (PEAR)­" was initiated after the disastrous 1999 Chi-Chi earthquake (Mw 7.6). As part of the PEAR, a dense continuous GPS array consisting of 150 new and about 50 pre-existing stations will be completed in the Taiwan area by the end of 2005 through a joint effort by the Central Weather Bureau and the Institute of Earth Sciences, Academia Sinica. The 50 new stations are going to be evenly distributed around the Taiwan Island. The other

  13. Earthquakes and plate tectonics.

    USGS Publications Warehouse

    Spall, H.

    1982-01-01

    Earthquakes occur at the following three kinds of plate boundary: ocean ridges where the plates are pulled apart, margins where the plates scrape past one another, and margins where one plate is thrust under the other. Thus, we can predict the general regions on the earth's surface where we can expect large earthquakes in the future. We know that each year about 140 earthquakes of magnitude 6 or greater will occur within this area which is 10% of the earth's surface. But on a worldwide basis we cannot say with much accuracy when these events will occur. The reason is that the processes in plate tectonics have been going on for millions of years. Averaged over this interval, plate motions amount to several mm per year. But at any instant in geologic time, for example the year 1982, we do not know, exactly where we are in the worldwide cycle of strain build-up and strain release. Only by monitoring the stress and strain in small areas, for instance, the San Andreas fault, in great detail can we hope to predict when renewed activity in that part of the plate tectonics arena is likely to take place. -from Author

  14. Pixelated neutron image plates

    NASA Astrophysics Data System (ADS)

    Schlapp, M.; Conrad, H.; von Seggern, H.

    2004-09-01

    Neutron image plates (NIPs) have found widespread application as neutron detectors for single-crystal and powder diffraction, small-angle scattering and tomography. After neutron exposure, the image plate can be read out by scanning with a laser. Commercially available NIPs consist of a powder mixture of BaFBr : Eu2+ and Gd2O3 dispersed in a polymer matrix and supported by a flexible polymer sheet. Since BaFBr : Eu2+ is an excellent x-ray storage phosphor, these NIPs are particularly sensitive to ggr-radiation, which is always present as a background radiation in neutron experiments. In this work we present results on NIPs consisting of KCl : Eu2+ and LiF that were fabricated into ceramic image plates in which the alkali halides act as a self-supporting matrix without the necessity for using a polymeric binder. An advantage of this type of NIP is the significantly reduced ggr-sensitivity. However, the much lower neutron absorption cross section of LiF compared with Gd2O3 demands a thicker image plate for obtaining comparable neutron absorption. The greater thickness of the NIP inevitably leads to a loss in spatial resolution of the image plate. However, this reduction in resolution can be restricted by a novel image plate concept in which a ceramic structure with square cells (referred to as a 'honeycomb') is embedded in the NIP, resulting in a pixelated image plate. In such a NIP the read-out light is confined to the particular illuminated pixel, decoupling the spatial resolution from the optical properties of the image plate material and morphology. In this work, a comparison of experimentally determined and simulated spatial resolutions of pixelated and unstructured image plates for a fixed read-out laser intensity is presented, as well as simulations of the properties of these NIPs at higher laser powers.

  15. Microscopic Materials on a Magnet

    NASA Technical Reports Server (NTRS)

    2008-01-01

    These images show a comparison of the weak magnet OM7 from the Optical Microscope on NASA's Phoenix Mars Lander before (left) and after (right) soil deposition.

    The microscope took the left image during Phoenix's Sol 15 (June 10, 2008) and the right image during Sol 21 (Jun 16, 2008).

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  16. Long working distance interference microscope

    DOEpatents

    Sinclair, Michael B.; DeBoer, Maarten P.; Smith, Norman F.

    2004-04-13

    Disclosed is a long working distance interference microscope suitable for three-dimensional imaging and metrology of MEMS devices and test structures on a standard microelectronics probe station. The long working distance of 10-30 mm allows standard probes or probe cards to be used. This enables nanometer-scale 3-D height profiles of MEMS test structures to be acquired across an entire wafer. A well-matched pair of reference/sample objectives is not required, significantly reducing the cost of this microscope, as compared to a Linnik microinterferometer.

  17. Hydrothermal synthesis of hydroxyapatite plates prepared using low molecular weight heparin (LMWH).

    PubMed

    Rajeswari, A; Kumar, V Ganesh; Karthick, V; Dhas, T Stalin; Potluri, Sri Lakshmi

    2013-11-01

    Materials with enhanced physical and biological properties have been used for biomedical applications and can be developed by functionalizing them using various components. Hydroxyapatite (HAP), among other available synthetic material, serves as one of the best tools in orthopaedics and ceramic coatings. The porous structure of HAP helps in bone cell regeneration, chemical integration of bone and also favours the interaction between bone and tissues. Herein, we have demonstrated a simple procedure for the synthesis of HAP using low molecular weight heparin (LMWH), a structural analogue of bone heparan sulphate proteoglycan. The presence of small sized HAP plates with well-defined structures was revealed using electron microscopic analysis. The phase purity of the synthesized HAP was evaluated using X-ray diffraction pattern obtained before and after immersion in simulated body fluid (SBF).

  18. Recent Opportunity Microscopic Imager Results

    NASA Astrophysics Data System (ADS)

    Herkenhoff, K. E.; Arvidson, R. E.; Jolliff, B. L.; Yingst, R.; Team, A.

    2013-12-01

    Opportunity arrived at exposures of Endeavour crater rim rocks in August 2011, on a hill dubbed 'Cape York.' These rocks have been the goal of exploration by Opportunity for the past few years because spectral evidence for phyllosilicates was observed at this location in orbital remote sensing data. As Opportunity circum¬navigated Cape York, the Microscopic Imager (MI) was used to examine the fine-scale textures of various soils and rocky outcrops. As reported previously, Opportunity discovered multiple bright linear features along the western periphery of Cape York that have been interpreted as veins of Ca sulfate deposited in fractures within the bedrock of Cape York. Opportunity then explored the northern and eastern sides of Cape York, including the area around 'Matijevic Hill' that shows evidence for phyllosilicates in CRISM data acquired from the Mars Reconnaissance Orbiter. One of the first outcrops examined near Matijevic Hill, dubbed 'Kirkwood,' is dominated by millimeter-size spherules. Unlike the hematite-rich concretions observed by Opportunity on Meridiani Planum, the aggregated 'newberries' in the Kirkwood exposure display internal structure and resistant rims. Compositionally, the spherule-rich rock is very similar to a nearby spherule-poor outcrop dubbed 'Whitewater Lake.' Thus these spherules have a more basalt-like composition compared to the hematite-rich concretions of the Burns Formation. The origin of the Kirkwood outcrop is uncertain, but the setting on the rim of the 22-km diameter Endeavour crater suggests that perhaps impact melting was involved in lapilli formation, possibly followed by mobilization and sorting in the ejecta blanket. Alternatively, the newberries may be diagenetic iron oxide concretions that are less well cemented than the 'blueberries' of the younger sulfate-rich Burns Formation. The Whitewater Lake outcrops contain the phyllosilicate phases observed from orbit, and are the oldest materials yet investigated by

  19. Chemical and structural analyses of titanium plates retrieved from patients.

    PubMed

    Pinto, C M S A; Asprino, L; de Moraes, M

    2015-08-01

    The aim of this study was to evaluate the microscopic structure and chemical composition of titanium bone plates and screws retrieved from patients with a clinical indication and to relate the results to the clinical conditions associated with the removal of these devices. Osteosynthesis plates and screws retrieved from 30 patients between January 2010 and September 2013 were studied by metallographic, gas, and energy dispersive X-ray (EDX) analyses and the medical records of these patients were reviewed. Forty-eight plates and 238 screws were retrieved. The time elapsed between plate and screw insertion and removal ranged between 11 days and 10 years. Metallographic analysis revealed that all the plates were manufactured from commercially pure titanium (CP-Ti). The screw samples analyzed consisted of Ti-6Al-4V alloy, except four samples, which consisted of CP-Ti. Titanium plates studied by EDX analysis presented greater than 99.7% titanium by mass. On gas analysis of Ti-6Al-4V screws, three samples were outside the standard values. One CP-Ti screw sample and one plate sample also presented an oxygen analysis value above the standard. The results indicated that the physical properties and chemical compositions of the plates and screws did not correspond with the need to remove these devices or the time of retention.

  20. Laser confocal microscope with wavelet-profiled point spread function

    NASA Astrophysics Data System (ADS)

    Romero, Mary Jacquiline; Bautista, Godofredo; Daria, Vincent Ricardo; Saloma, Caesar

    2010-04-01

    We report a laser-scanning confocal reflectance microscope with a wavelet-profiled point spread function (PSF) for rapid multi-resolution extraction and analysis of microscopic object features. The PSF is generated via holography by encoding a π-phase shifting disk unto a collimated laser beam via a phase-only spatial light modulator (SLM) that is positioned at the pupil plane of the focusing objective lens. Scaling of the transverse PSF distribution is achieved by selecting a suitable ratio of the π-phase shifting disk radius and the pupil aperture radius. With one and the same objective lens and one SLM to control the phase profile of the pupil function, we produce amplitude PSF distributions that are accurate scaled representations of the circularly-symmetric Mexican hat mother wavelet.

  1. Advanced methods of microscope control using μManager software

    PubMed Central

    Edelstein, Arthur D.; Tsuchida, Mark A.; Amodaj, Nenad; Pinkard, Henry; Vale, Ronald D.; Stuurman, Nico

    2014-01-01

    μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging. PMID:25606571

  2. Silver plating ensures reliable diffusion bonding of dissimilar metals

    NASA Technical Reports Server (NTRS)

    1967-01-01

    Dissimilar metals are reliably joined by diffusion bonding when the surfaces are electroplated with silver. The process involves cleaning and etching, anodization, silver striking, and silver plating with a conventional plating bath. It minimizes the formation of detrimental intermetallic phases and provides greater tolerance of processing parameters.

  3. Designing Assemblies Of Plates

    NASA Technical Reports Server (NTRS)

    Williams, F. W.; Kennedy, D.; Butler, R.; Aston, G.; Anderson, M. S.

    1992-01-01

    VICONOPT calculates vibrations and instabilities of assemblies of prismatic plates. Designed for efficient, accurate analysis of buckling and vibration, and for optimum design of panels of composite materials. Written in FORTRAN 77.

  4. Plate tectonics: Metamorphic myth

    NASA Astrophysics Data System (ADS)

    Korenaga, Jun

    2016-01-01

    Clear evidence for subduction-induced metamorphism, and thus the operation of plate tectonics on the ancient Earth has been lacking. Theoretical calculations indicate that we may have been looking for something that cannot exist.

  5. Violin plate modes.

    PubMed

    Gough, Colin

    2015-01-01

    As the first step toward developing a generic model for the acoustically radiating vibrational modes of the violin and related instruments, the modes of both freely supported and edge-constrained top and back plates have been investigated as functions of shape, arching height, elastic anisotropy, the f-holes and associated island area, thickness graduations, and the additional boundary constraints of the ribs, soundpost, and bass-bar present in the assembled instrument. Comsol shell structure finite element software has been used as a quasi-experimental tool, with physical and geometric properties varied smoothly, often over several orders of magnitude, allowing the development of the plate modes to be followed continuously from those of an initially square plate to those of doubly-arched, guitar-shaped, orthotropic plates and their dependence on all the above factors.

  6. Tectonic Plate Movement.

    ERIC Educational Resources Information Center

    Landalf, Helen

    1998-01-01

    Presents an activity that employs movement to enable students to understand concepts related to plate tectonics. Argues that movement brings topics to life in a concrete way and helps children retain knowledge. (DDR)

  7. Reduction of astrometric plates

    NASA Technical Reports Server (NTRS)

    Stock, J.

    1984-01-01

    A rapid and accurate method for the reduction of comet or asteroid plates is described. Projection equations, scale length correction, rotation of coordinates, linearization, the search for additional reference stars, and the final solution are examined.

  8. What's On Your Plate?

    MedlinePlus

    ... Table of Contents What's On Your Plate? Smart Food Choices for Healthy Aging www.nia.nih.gov/health/ ... calories in" and "calories out," and making good food choices as you age. Shopping Tips See how planning ...

  9. Feynman's wobbling plate

    NASA Astrophysics Data System (ADS)

    Tuleja, Slavomir; Gazovic, Boris; Tomori, Alexander; Hanc, Jozef

    2007-03-01

    In the book Surely You Are Joking, Mr. Feynman! Richard Feynman tells a story of a Cornell cafeteria plate being tossed into the air. As the plate spun, it wobbled. Feynman noticed a relation between the two motions. He solved the motion of the plate by using the Lagrangian approach. This solution didn't satisfy him. He wanted to understand the motion of the plate by analyzing the motion of its individual particles and the forces acting on them. He was successful, but he didn't tell us how he did it. We provide an elementary explanation for the two-to-one ratio of wobble to spin frequencies, based on an analysis of the motion of the particles and the forces acting on them. We also demonstrate the power of numerical simulation and computer animation to provide insight into a physical phenomenon and guidance on how to do the analysis.

  10. Flat plate solar oven

    SciTech Connect

    Parikh, M.

    1981-01-01

    The construction of an Indian Rs. 186 (US $20.33) flat-plate solar oven is described. Detailed drawings are provided and relevant information on cooking times and temperature for different foods is given.

  11. The Gray Institute 'open' high-content, fluorescence lifetime microscopes.

    PubMed

    Barber, P R; Tullis, I D C; Pierce, G P; Newman, R G; Prentice, J; Rowley, M I; Matthews, D R; Ameer-Beg, S M; Vojnovic, B

    2013-08-01

    We describe a microscopy design methodology and details of microscopes built to this 'open' design approach. These demonstrate the first implementation of time-domain fluorescence microscopy in a flexible automated platform with the ability to ease the transition of this and other advanced microscopy techniques from development to use in routine biology applications. This approach allows easy expansion and modification of the platform capabilities, as it moves away from the use of a commercial, monolithic, microscope body to small, commercial off-the-shelf and custom made modular components. Drawings and diagrams of our microscopes have been made available under an open license for noncommercial use at http://users.ox.ac.uk/~atdgroup. Several automated high-content fluorescence microscope implementations have been constructed with this design framework and optimized for specific applications with multiwell plates and tissue microarrays. In particular, three platforms incorporate time-domain FLIM via time-correlated single photon counting in an automated fashion. We also present data from experiments performed on these platforms highlighting their automated wide-field and laser scanning capabilities designed for high-content microscopy. Devices using these designs also form radiation-beam 'end-stations' at Oxford and Surrey Universities, showing the versatility and extendibility of this approach.

  12. Switch on Micro*scope!

    ERIC Educational Resources Information Center

    Roland, Sarah; Bahr, Michele; Olendzenski, Lorraine; Patterson, David J.

    2005-01-01

    Scientists at the Marine Biological Laboratory in Woods Hole, Massachusetts, have created micro*scope, a free, searchable knowledge environment for exploring the microbial world. Microbiology can easily be incorporated into the curriculum, because microbial communities are easy to access. Organisms grow quickly, making certain arrays of…

  13. Scanning tunneling microscope nanoetching method

    DOEpatents

    Li, Yun-Zhong; Reifenberger, Ronald G.; Andres, Ronald P.

    1990-01-01

    A method is described for forming uniform nanometer sized depressions on the surface of a conducting substrate. A tunneling tip is used to apply tunneling current density sufficient to vaporize a localized area of the substrate surface. The resulting depressions or craters in the substrate surface can be formed in information encoding patterns readable with a scanning tunneling microscope.

  14. Nature Study with the Microscope.

    ERIC Educational Resources Information Center

    Sollberger, Dwight E.

    1991-01-01

    Identifies specific instruction difficulties, potential problems, solutions, and activities for successful use of microscopes in the classroom. Procedures are outlined for guiding students in creating their own slides with monocotyledon and dicotyledon stems, fern spores, stomata, lichens, and red onions. (MCO)

  15. Curriculum Guidelines for Microscopic Anatomy.

    ERIC Educational Resources Information Center

    Journal of Dental Education, 1993

    1993-01-01

    The American Association of Dental Schools' guidelines for curricula in microscopic anatomy offer an overview of the histology curriculum, note primary educational goals, outline specific content for general and oral histology, suggest prerequisites, and make recommendations for sequencing. Appropriate faculty and facilities are also suggested.…

  16. Chasing Meteors With a Microscope.

    ERIC Educational Resources Information Center

    Jones, Richard C.

    1993-01-01

    Describes types of meteors and micrometeorites that enter the Earth's atmosphere. Presents an activity where students collect micrometeorites with a strip of tape in an undisturbed outdoor area. After 24 hours, they examine the tape by sandwiching it between 2 glass slides and view through a microscope at 100X. (PR)

  17. The Biggest Microscopic Image Ever

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This is a mosaic of four individual frames taken by the microscopic imager that have been very carefully stitched together to reveal the entire 5-centimeter-diameter (almost 2-inch) hole left on the rock dubbed 'Humphrey.' The holes were created by the Mars Exploration Rover Spirit's rock abrasion tool. The mosaic, created on March 7, 2004, is the first of its kind of an abraded surface on Mars, and gave scientists their first ever microscopic imager view of the entire drilled area. While it is easy for the panoramic camera and the navigation cameras to fit an area this size into their field of view, the microscopic imager can only capture a portion of the ground area with each image.

    Scientists are interested in many of the small features on 'Humphrey' uncovered by the rock abrasion tool and made visible by the microscopic imager. The sinuous veins within the rock could be evidence that water was trickling through the material while it was deep underground, whereas the dark 'age spots' in the center of the hole may be crystals of the mineral olivine.

  18. Fractal multifiber microchannel plates

    NASA Technical Reports Server (NTRS)

    Cook, Lee M.; Feller, W. B.; Kenter, Almus T.; Chappell, Jon H.

    1992-01-01

    The construction and performance of microchannel plates (MCPs) made using fractal tiling mehtods are reviewed. MCPs with 40 mm active areas having near-perfect channel ordering were produced. These plates demonstrated electrical performance characteristics equivalent to conventionally constructed MCPs. These apparently are the first MCPs which have a sufficiently high degree of order to permit single channel addressability. Potential applications for these devices and the prospects for further development are discussed.

  19. Positive battery plate

    NASA Technical Reports Server (NTRS)

    Rowlette, John R. (Inventor)

    1985-01-01

    The power characteristics of a lead acid battery are improved by incorporating a dispersion of 1 to 10% by weight of a thermodynamically stable conductivity additive, such as conductive tin oxide coated glass fibers (34) of filamentary glass wool (42) in the positive active layer (32) carried on the grid (30) of the positive plate (16). Positive plate potential must be kept high enough to prevent reduction of the tin oxide to tin by utilizing an oversized, precharged positive paste.

  20. Long-working-distance incoherent-light interference microscope.

    PubMed

    Sinclair, Michael B; de Boer, Maarten P; Corwin, Alex D

    2005-12-20

    We describe the design and operation of a long-working-distance, incoherent light interference microscope that has been developed to address the growing demand for new microsystem characterization tools. The design of the new microscope is similar to that of a Linnik interference microscope and thus preserves the full working distance of the long-working-distance objectives utilized. However, in contrast to a traditional Linnik microscope, the new microscope does not rely on the use of matched objectives in the sample and the reference arms of the interferometer. An adjustable optical configuration has been devised that allows the total optical path length, wavefront curvature, and dispersion of the reference arm to be matched to the sample arm of the interferometer. The reference arm configuration can be adjusted to provide matching for 5x, 10x, and 20x long-working-distance objectives in the sample arm. In addition to retaining the full working distance of the sample arm objectives, the new design allows interference images to be acquired in situations in which intervening windows are necessary, such as occur with packaged microsystems, microfluidic devices, and cryogenic, vacuum, or environmental chamber studies of microsystem performance. The interference microscope is compatible with phase-shifting interferometry, vertical scanning interferometry, and stroboscopic measurement of dynamic processes.

  1. Long working-distance, incoherent light interference microscope

    SciTech Connect

    Sinclair, Michael B.; de Boer, Maarten Pieter; Corwin, Alex David

    2005-06-01

    We describe the design and operation of a long-working-distance, incoherent light interference microscope that has been developed to address the growing demand for new microsystem characterization tools. The design of the new microscope is similar to that of a Linnik interference microscope and thus preserves the full working distance of the long-working-distance objectives utilized. However, in contrast to a traditional Linnik microscope, the new microscope does not rely on the use of matched objectives in the sample and the reference arms of the interferometer. An adjustable optical configuration has been devised that allows the total optical path length, wavefront curvature, and dispersion of the reference arm to be matched to the sample arm of the interferometer. The reference arm configuration can be adjusted to provide matching for 5x, 10x, and 20x long-working-distance objectives in the sample arm. In addition to retaining the full working distance of the sample arm objectives, the new design allows interference images to be acquired in situations in which intervening windows are necessary, such as occur with packaged microsystems, microfluidic devices, and cryogenic, vacuum, or environmental chamber studies of microsystem performance. The interference microscope is compatible with phase-shifting interferometry, vertical scanning interferometry, and stroboscopic measurement of dynamic processes.

  2. The Scanning TMR Microscope for Biosensor Applications

    PubMed Central

    Vyas, Kunal N.; Love, David M.; Ionescu, Adrian; Llandro, Justin; Kollu, Pratap; Mitrelias, Thanos; Holmes, Stuart; Barnes, Crispin H. W.

    2015-01-01

    We present a novel tunnel magnetoresistance (TMR) scanning microscope set-up capable of quantitatively imaging the magnetic stray field patterns of micron-sized elements in 3D. By incorporating an Anderson loop measurement circuit for impedance matching, we are able to detect magnetoresistance changes of as little as 0.006%/Oe. By 3D rastering a mounted TMR sensor over our magnetic barcodes, we are able to characterise the complex domain structures by displaying the real component, the amplitude and the phase of the sensor’s impedance. The modular design, incorporating a TMR sensor with an optical microscope, renders this set-up a versatile platform for studying and imaging immobilised magnetic carriers and barcodes currently employed in biosensor platforms, magnetotactic bacteria and other complex magnetic domain structures of micron-sized entities. The quantitative nature of the instrument and its ability to produce vector maps of magnetic stray fields has the potential to provide significant advantages over other commonly used scanning magnetometry techniques. PMID:25849347

  3. Caribbean plate interactions

    SciTech Connect

    Ball, M. )

    1993-02-01

    Vector analysis of plate motions, derived from studies of Atlantic magnetic lineations and fracture zone trends, indicates the following relative movements between the Caribbean, North American, and South American Plates. (1) During Early Jurassic to Early Cretaceous, the North American Plate moved 1900 km westward and 900 km northward relative to the South American Plate. A broad zone including the Caribbean region, i.e., the zone between the North and South America Plates, was a site of left-lateral shear and north-south extension. (2) During Early Cretaceous to Late Cretaceous, the North American Mate moved an additional 1200 km westward relative to South America across this zone. (3) During Late Cretaceous to the end of the Eocene, the North American Plate moved 200 km westward and 400 km northward relative to the South American Plate. (4) From the end of the Eocene to near the end of the Miocene, North America converged on South America some 200 km and moved 100 km eastward relative to it. Through the Mesozoic and earliest Tertiary history of the Caribbean, the region was a shear zone within which left-lateral displacement exceeded 3000 km and north-south extension exceeded 1300 km. In regard to time, 80% of the history of the Caribbean region is one of north-south extension and left-lateral shear. In terms of space, 97% of the shear is left-lateral and the ratio of divergence versus convergence is 7 to 1. Thus, characterizing the Caribbean region, and the Atlantic to its east, as a zone of north-south extension and left-lateral shear, is a fair generalization.

  4. Spirit Says Goodbye to 'Home Plate'

    NASA Technical Reports Server (NTRS)

    2006-01-01

    For the past several weeks, Spirit has been examining spectacular layered rocks exposed at 'Home Plate.' The rover has been driving around the northern and eastern edges of Home Plate, on the way to 'McCool Hill.' Before departing, Spirit took this image showing some of the most complex layering patterns seen so far at this location.

    The layered nature of these rocks presents new questions for the rover team. In addition to their chemical properties, which scientists can study using Spirit's spectrometers, these rocks record a detailed history of the physical properties that formed them. In the center of this image, one group of layers slopes downward to the right. The layers above and below this group are more nearly horizontal. Where layers of different orientations intersect, other layers are truncated. This indicates that there were complex patterns of alternating erosion and deposition occurring when these layers were being deposited. Similar patterns can be found in some sedimentary rocks on Earth. Physical relationships among the various layers exposed at Home Plate are crucial evidence in understanding how these Martian rocks formed. Scientists suspect that the rocks at Home Plate were formed in the aftermath of a volcanic explosion or impact event, and they are investigating the possibility that wind may also have played a role in redistributing materials after such an event.

    Images like this one from panoramic camera (Pancam), which shows larger-scale layering, as well as those from the microscopic imager, which reveal the individual sand-sized grains that make up these rocks, are essential to understanding the geologic history of Home Plate.

    This view is an approximately true-color rendering that combines separate images taken through the Pancam's 753-nanometer, 535-namometer, and 432-nanometer filters during Spirit's 774th Martian day (March 8, 2006).

  5. Shear Brillouin light scattering microscope

    PubMed Central

    Kim, Moonseok; Besner, Sebastien; Ramier, Antoine; Kwok, Sheldon J. J.; An, Jeesoo; Scarcelli, Giuliano; Yun, Seok Hyun

    2016-01-01

    Brillouin spectroscopy has been used to characterize shear acoustic phonons in materials. However, conventional instruments had slow acquisition times over 10 min per 1 mW of input optical power, and they required two objective lenses to form a 90° scattering geometry necessary for polarization coupling by shear phonons. Here, we demonstrate a confocal Brillouin microscope capable of detecting both shear and longitudinal phonons with improved speeds and with a single objective lens. Brillouin scattering spectra were measured from polycarbonate, fused quartz, and borosilicate in 1-10 s at an optical power level of 10 mW. The elastic constants, phonon mean free path and the ratio of the Pockels coefficients were determined at microscopic resolution. PMID:26832263

  6. Nanocarpets for Trapping Microscopic Particles

    NASA Technical Reports Server (NTRS)

    Noca, Flavio; Chen, Fei; Hunt, Brian; Bronikowski, Michael; Hoenk, Michael; Kowalczyk, Robert; Choi, Daniel

    2004-01-01

    Nanocarpets that is, carpets of carbon nanotubes are undergoing development as means of trapping microscopic particles for scientific analysis. Examples of such particles include inorganic particles, pollen, bacteria, and spores. Nanocarpets can be characterized as scaled-down versions of ordinary macroscopic floor carpets, which trap dust and other particulate matter, albeit not purposefully. Nanocarpets can also be characterized as mimicking both the structure and the particle-trapping behavior of ciliated lung epithelia, the carbon nanotubes being analogous to cilia. Carbon nanotubes can easily be chemically functionalized for selective trapping of specific particles of interest. One could, alternatively, use such other three-dimensionally-structured materials as aerogels and activated carbon for the purposeful trapping of microscopic particles. However, nanocarpets offer important advantages over these alternative materials: (1) Nanocarpets are amenable to nonintrusive probing by optical means; and (2) Nanocarpets offer greater surface-to-volume ratios.

  7. Hyperbaric hydrothermal atomic force microscope

    DOEpatents

    Knauss, Kevin G.; Boro, Carl O.; Higgins, Steven R.; Eggleston, Carrick M.

    2002-01-01

    A hyperbaric hydrothermal atomic force microscope (AFM) is provided to image solid surfaces in fluids, either liquid or gas, at pressures greater than normal atmospheric pressure. The sample can be heated and its surface imaged in aqueous solution at temperatures greater than 100.degree. C. with less than 1 nm vertical resolution. A gas pressurized microscope base chamber houses the stepper motor and piezoelectric scanner. A chemically inert, flexible membrane separates this base chamber from the sample cell environment and constrains a high temperature, pressurized liquid or gas in the sample cell while allowing movement of the scanner. The sample cell is designed for continuous flow of liquid or gas through the sample environment.

  8. Hyperbaric Hydrothermal Atomic Force Microscope

    DOEpatents

    Knauss, Kevin G.; Boro, Carl O.; Higgins, Steven R.; Eggleston, Carrick M.

    2003-07-01

    A hyperbaric hydrothermal atomic force microscope (AFM) is provided to image solid surfaces in fluids, either liquid or gas, at pressures greater than normal atmospheric pressure. The sample can be heated and its surface imaged in aqueous solution at temperatures greater than 100.degree. C. with less than 1 nm vertical resolution. A gas pressurized microscope base chamber houses the stepper motor and piezoelectric scanner. A chemically inert, flexible membrane separates this base chamber from the sample cell environment and constrains a high temperature, pressurized liquid or gas in the sample cell while allowing movement of the scanner. The sample cell is designed for continuous flow of liquid or gas through the sample environment.

  9. Lensless multispectral digital in-line holographic microscope

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; McDonnell, Susan; Sheridan, John T.

    2011-12-01

    An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

  10. Lensless multispectral digital in-line holographic microscope.

    PubMed

    Ryle, James P; McDonnell, Susan; Sheridan, John T

    2011-12-01

    An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

  11. Subsurface Imaging with the Scanning Microwave Microscope

    NASA Astrophysics Data System (ADS)

    Kopanski, Joseph; You, Lin; Michelson, Jonathan; Hitz, Emily; Obeng, Yaw; Back End of the Line Reliability; Metrology Project Team

    2015-03-01

    The scanning microwave microscope (SMM) forms images from the reflected amplitude and phase of an incident RF (~ 2.3 GHz) signal. The reflected signal is a function of the properties of the tip-sample contact, but can also be influenced by buried interfaces and subsurface variations of the sample permittivity. This mechanism allows limited imaging of conductors buried within dielectrics, voids within metal, or multiple metal layers with different permittivity. Subsurface SMM data acquisition modes include passive and various active data acquisition modes. The theory of sub-surface imaging with SMM and COMSOL multi-physics simulations of specific situations will be presented. Measurements of specifically designed test structures and correlation with simulations show the sensitivity and resolution of the technique applied to imaging subsurface metal lines embedded in dielectric. Applications include metrology for back end of the line (BEOL) multi-level metallization and three-dimensional integrated circuits (3D-ICs).

  12. Microscopic Description of Scission Configurations

    SciTech Connect

    Dubray, N.; Goutte, H.; Berger, J. F.

    2007-02-26

    Properties of 226Th, 256Fm, 258Fm and 260Fm nuclei in the scission region are described using a full-microscopic Hartree-Fock-Bogoliubov approach with the effective Gogny nucleon-nucleon interaction. In a first step, the Potential Energy Surfaces are computed in the (q 20, q30) plane, the scission lines are found, fulfilling a given criterion on the density in the nuclear neck. Finally a few properties of the fragments along this line are presented.

  13. Apparatus Would Stain Microscope Slides

    NASA Technical Reports Server (NTRS)

    Breeding, James D.

    1993-01-01

    Proposed apparatus meters specific amounts of fluid out of containers at specific times to stain microscope slides. Intended specifically for semiautomated staining of microbiological and hematological samples in microgravity, leakproof apparatus used in other environments in which technicians have little time to allocate to staining procedures and/or exposure to toxic staining agents or to micro-organisms to be stained hazardous. Apparatus adapted to perform almost any staining procedure and accommodates multiple staining reagents, useful for small or remote clinical laboratories.

  14. Cadmium plating replacements

    SciTech Connect

    Nelson, M.J.; Groshart, E.C.

    1995-03-01

    The Boeing Company has been searching for replacements to cadmium plate. Two alloy plating systems seem close to meeting the needs of a cadmium replacement. The two alloys, zinc-nickel and tin-zinc are from alloy plating baths; both baths are neutral pH. The alloys meet the requirements for salt fog corrosion resistance, and both alloys excel as a paint base. Currently, tests are being performed on standard fasteners to compare zinc-nickel and tin-zinc on threaded hardware where cadmium is heavily used. The Hydrogen embrittlement propensity of the zinc-nickel bath has been tested, and just beginning for the tin-zinc bath. Another area of interest is the electrical properties on aluminum for tin-zinc and will be discussed. The zinc-nickel alloy plating bath is in production in Boeing Commercial Airplane Group for non-critical low strength steels. The outlook is promising that these two coatings will help The Boeing Company significantly reduce its dependence on cadmium plating.

  15. Cadmium plating replacements

    NASA Technical Reports Server (NTRS)

    Nelson, Mary J.; Groshart, Earl C.

    1995-01-01

    The Boeing Company has been searching for replacements to cadmium plate. Two alloy plating systems seem close to meeting the needs of a cadmium replacement. The two alloys, zinc-nickel and tin-zinc are from alloy plating baths; both baths are neutral pH. The alloys meet the requirements for salt fog corrosion resistance, and both alloys excel as a paint base. Currently, tests are being performed on standard fasteners to compare zinc-nickel and tin-zinc on threaded hardware where cadmium is heavily used. The Hydrogen embrittlement propensity of the zinc-nickel bath has been tested, and just beginning for the tin-zinc bath. Another area of interest is the electrical properties on aluminum for tin-zinc and will be discussed. The zinc-nickel alloy plating bath is in production in Boeing Commercial Airplane Group for non-critical low strength steels. The outlook is promising that these two coatings will help The Boeing Company significantly reduce its dependence on cadmium plating.

  16. Acoustic Plate Mode sensing in liquids based on free and electrically shorted plate surfaces.

    PubMed

    Anisimkin, V I; Caliendo, C; Verona, E

    2016-05-01

    The sensing behavior to liquids for Acoustic Plate Modes (APMs) propagating along 64°Y, 90°X LiNbO3 plate was investigated vs. two electric boundary conditions. The changes in the APMs phase velocity and attenuation were measured upon exposure to different liquids wetting one of the surfaces of the plate, either free or electrically shorted by a thin conductive Al layer. The experimental data confirm that the presence of a metallic layer covering one of the plate surfaces affects the viscosity and temperature sensitivity of the device. The differences between the sensor response for various liquids, with free or metalized faces, are interpreted in terms of the APM polarization.

  17. Microscope Image of Scavenged Particles

    NASA Technical Reports Server (NTRS)

    2008-01-01

    This image from NASA's Phoenix Mars Lander's Optical Microscope shows a strongly magnetic surface which has scavenged particles from within the microscope enclosure before a sample delivery from the lander's Robotic Arm. The particles correspond to the larger grains seen in fine orange material that makes up most of the soil at the Phoenix site. They vary in color, but are of similar size, about one-tenth of a millimeter.

    As the microscope's sample wheel moved during operation, these particles also shifted, clearing a thin layer of the finer orange particles that have also been collected. Together with the previous image, this shows that the larger grains are much more magnetic than the fine orange particles with a much larger volume of the grains being collected by the magnet. The image is 2 milimeters across.

    It is speculated that the orange material particles are a weathering product from the larger grains, with the weathering process both causing a color change and a loss of magnetism.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by JPL, Pasadena, Calif. Spacecraft development was by Lockheed Martin Space Systems, Denver.

  18. Duties to Extraterrestrial Microscopic Organisms

    NASA Astrophysics Data System (ADS)

    Cockell, C. S.

    Formulating a normative axiology for the treatment of extraterrestrial microscopic organisms, should they ever be found, requires an extension of environmental ethics to beyond the Earth. Using an ethical framework for the treatment of terrestrial micro-organisms, this paper elaborates a similar ethic for the treatment of extraterrestrial microscopic organisms. An ethic of `teloempathy' allows for the moral considerability of any organism that has `interests', based on rudimentary qualities of conativism, and therefore allows for an identical treatment of all life, related or not related to life on Earth. Although, according to this ethic, individual extraterrestrial microscopic organisms have a good of their own and even `rights', at this level the ethic can only be theoretical, allowing for the inevitable destruction of many individual organisms during the course of human exploratory missions, similarly to the daily destruction of microbes by humans on Earth. A holistic teloempathy, an operative ethic, not only provides a framework for human exploration, but it also has important implications for planetary protection and proposals to implement planetary-scale atmospheric alterations on other bodies. Even prior to the discovery of extraterrestrial life, or the discovery of a complete absence of such life, this exercise yields important insights into the moral philosophy that guides our treatment of terrestrial micro-organisms.

  19. Compact Microscope Imaging System Developed

    NASA Technical Reports Server (NTRS)

    McDowell, Mark

    2001-01-01

    The Compact Microscope Imaging System (CMIS) is a diagnostic tool with intelligent controls for use in space, industrial, medical, and security applications. The CMIS can be used in situ with a minimum amount of user intervention. This system, which was developed at the NASA Glenn Research Center, can scan, find areas of interest, focus, and acquire images automatically. Large numbers of multiple cell experiments require microscopy for in situ observations; this is only feasible with compact microscope systems. CMIS is a miniature machine vision system that combines intelligent image processing with remote control capabilities. The software also has a user-friendly interface that can be used independently of the hardware for post-experiment analysis. CMIS has potential commercial uses in the automated online inspection of precision parts, medical imaging, security industry (examination of currency in automated teller machines and fingerprint identification in secure entry locks), environmental industry (automated examination of soil/water samples), biomedical field (automated blood/cell analysis), and microscopy community. CMIS will improve research in several ways: It will expand the capabilities of MSD experiments utilizing microscope technology. It may be used in lunar and Martian experiments (Rover Robot). Because of its reduced size, it will enable experiments that were not feasible previously. It may be incorporated into existing shuttle orbiter and space station experiments, including glove-box-sized experiments as well as ground-based experiments.

  20. Development of an automated MODS plate reader to detect early growth of Mycobacterium tuberculosis.

    PubMed

    Comina, G; Mendoza, D; Velazco, A; Coronel, J; Sheen, P; Gilman, R H; Moore, D A J; Zimic, M

    2011-06-01

    In this work, an automated microscopic observation drug susceptibility (MODS) plate reader has been developed. The reader automatically handles MODS plates and after autofocussing digital images are acquired of the characteristic microscopic cording structures of Mycobacterium tuberculosis, which are the identification method utilized in the MODS technique to detect tuberculosis and multidrug resistant tuberculosis. In conventional MODS, trained technicians manually move the MODS plate on the stage of an inverted microscope while trying to locate and focus upon the characteristic microscopic cording colonies. In centres with high tuberculosis diagnostic demand, sufficient time may not be available to adequately examine all cultures. An automated reader would reduce labour time and the handling of M. tuberculosis cultures by laboratory personnel. Two hundred MODS culture images (100 from tuberculosis positive and 100 from tuberculosis negative sputum samples confirmed by a standard MODS reading using a commercial microscope) were acquired randomly using the automated MODS plate reader. A specialist analysed these digital images with the help of a personal computer and designated them as M. tuberculosis present or absent. The specialist considered four images insufficiently clear to permit a definitive reading. The readings from the 196 valid images resulted in a 100% agreement with the conventional nonautomated standard reading. The automated MODS plate reader combined with open-source MODS pattern recognition software provides a novel platform for high throughput automated tuberculosis diagnosis.

  1. Scanned probe microscope for biological applications

    NASA Astrophysics Data System (ADS)

    Baiburin, Vil B.; Konnov, Nikolai P.; Shcherbakov, Anatolyi A.; Malakhaeva, Alina N.; Zadnova, Svetlana P.; Volkov, Yuri P.

    1997-12-01

    In our biophysical laboratory has been developed a new scanned probe microscope (SPM) for biological application. The SPM allows to investigate a biological samples' surface by means of three different near field microscopes: scanning tunneling microscope (STM), atomic force microscope (AFM) and near field scanning optical microscope (NSOM). The SPM is very rigid and can be operated in ordinary laboratory without any vibration isolation. The scanning area of the microscope is about 10 by 10 micrometers. Some different biological objects were visualized by means of the SPM viz. bacteria (E. Coli, plague, cholera, staphylococcus), macromolecules (DNA, plague proteins) and phage (T2).

  2. Bipolar battery plate

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor)

    1987-01-01

    A liquid-impermeable plate (10) having through-plate conductivity with essentially zero resistance comprises an insulator sheet (12) having a series of spaced perforations (14) each of which contains a metal element (16) sealingly received into the perforation (14). A low-cost plate can readily be manufactured by punching a thermoplastic sheet (40) such as polypropylene with a punching tool (52), filling the apertures with led spheres (63) having a diameter smaller than the holes (50) but larger than the thickness of the sheet, sweeping excess spheres (62) off the sheet with a doctor blade (60) and then pressing a heated platen (74) onto the sheet to swage the spheres into a cylindrical shape and melt the surrounding resin to form a liquid-impermeable collar (4) sealing the metal into the sheet.

  3. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  4. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  5. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  6. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  7. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  8. Plate-mantle coupling from post-Pangea plate kinematics

    NASA Astrophysics Data System (ADS)

    Zahirovic, Sabin; Dietmar Müller, R.; Seton, Maria; Flament, Nicolas

    2015-04-01

    Convection in the Earth's mantle that involves plates at the surfaces gives rise to plate velocities that vary through time and depend on the balance of plate boundary forces, with the present-day providing a snapshot of this ongoing process. However, present-day plate velocities do not capture plate behaviour over geologically representative timeframes and thus cannot be used to evaluate factors limiting plate velocities. Previous studies investigated the effects of continental keels on plate speeds by either using the present-day snapshot or a limited number of reconstructed plate configurations, often leading to conflicting results. For example, an early assumption was that continental keels (especially cratons) were unlikely to impede fast plate motions because India's velocity approached ~20 cm/yr in the Eocene prior to the collision with Eurasia. We employ a modern plate reconstruction approach with evolving global topological plate boundaries for the post-Pangea timeframe (since 200 Ma) to evaluate factors controlling plate velocities. Plate boundary configurations and plate velocities are extracted from the open-source and cross-platform plate reconstruction package GPlates (www.gplates.org) at 1 Myr intervals. For each plate, at each timestep, the area of continental and cratonic lithosphere is calculated to evaluate the effect on plate velocities. Our results support that oceanic plates tend to be 2-3 times faster than plates with large portion of continental plate area, consistent with predictions of numerical models of mantle convection. The fastest plates (~8.5 cm/yr RMS) are dominated by oceanic plate area and high subducting portion of plate perimeter, while the slowest plates (~2.6-2.8 cm/yr RMS) are dominated by continental plate area and bounded by transforms and mid-oceanic ridge segments. Importantly, increasing cratonic fractions (both Proterozoic and Archean lithosphere) significantly impede plate velocities, suggesting that deep continental

  9. Second-generation zone plate antenna design

    NASA Astrophysics Data System (ADS)

    Wiltse, James C.

    1999-11-01

    A well-designed phase correcting Fresnel zone plate antenna can provide performance superior to a lens or, in some cases, a paraboloid antenna, particularly at millimeter wavelengths. This paper discusses design considerations and includes approaches to give improved characteristics, such as greater efficiency or higher gain. The approaches include the use of quarter-wave or better correction, thickness designs that permit the central zone and other zones to be air dielectric (for lower losses), and the use of low dielectric constant materials to reduce surface reflections and multiple reflections. At higher millimeter-wave or sub- millimeter wavelengths low loss materials are important. More sophisticated zoning is described, as well as the use of a compromise thickness to compensate for the fact that refraction of waves at the surfaces causes the path lengths through the zone plate to be different at different angles of incidence. Multiple-band zone plates are discussed.

  10. Development of Low-Cost Inverted Microscope to Detect Early Growth of Mycobacterium tuberculosis in MODS Culture

    PubMed Central

    Zimic, Mirko; Velazco, Abner; Comina, Germán; Coronel, Jorge; Fuentes, Patricia; Luna, Carmen G.; Sheen, Patricia; Gilman, Robert H.; Moore, David A. J.

    2010-01-01

    Background The microscopic observation drug susceptibility (MODS) assay for rapid, low-cost detection of tuberculosis and multidrug resistant tuberculosis depends upon visualization of the characteristic cording colonies of Mycobacterium tuberculosis in liquid media. This has conventionally required an inverted light microscope in order to inspect the MODS culture plates from below. Few tuberculosis laboratories have this item and the capital cost of $5,000 for a high-end microscope could be a significant obstacle to MODS roll-out. Methodology We hypothesized that the precise definition provided by costly high-specification inverted light microscopes might not be necessary for pattern recognition. Significance In this work we describe the development of a low-cost artesenal inverted microscope that can operate in both a standard or digital mode to effectively replace the expensive commercial inverted light microscope, and an integrated system that could permit a local and remote diagnosis of tuberculosis. PMID:20351778

  11. Reduced Plating Ignitron

    NASA Technical Reports Server (NTRS)

    Polzin, Kurt A (Inventor); Pearson, J Boise (Inventor)

    2014-01-01

    An ignitron apparatus has an airtight tubular housing having a first sealed end and a second sealed end. An anode is connected at the first sealed end, projecting into the housing, and a recess at the second sealed and forms a well which contains a quantity of liquid gallium or gallium alloy making up the cathode. An ignitor projects through the liquid metal and into the housing. The inner surface of the housing includes at least one plating-reduction structure to prevent electrical shorting of the apparatus caused by plating of the liquid metal.

  12. NICKEL PLATING PROCESS

    DOEpatents

    Hoover, T.B.; Zava, T.E.

    1959-05-12

    A simplified process is presented for plating nickel by the vapor decomposition of nickel carbonyl. In a preferred form of the invention a solid surface is nickel plated by subjecting the surface to contact with a mixture containing by volume approximately 20% nickel carbonyl vapor, 2% hydrogen sulfide and .l% water vapor or 1% oxygen and the remainder carbon dioxide at room temperature until the desired thickness of nickel is obtained. The advantage of this composition over others is that the normally explosive nickel carbonyl is greatly stabilized.

  13. License plate detection algorithm

    NASA Astrophysics Data System (ADS)

    Broitman, Michael; Klopovsky, Yuri; Silinskis, Normunds

    2013-12-01

    A novel algorithm for vehicle license plates localization is proposed. The algorithm is based on pixel intensity transition gradient analysis. Near to 2500 natural-scene gray-level vehicle images of different backgrounds and ambient illumination was tested. The best set of algorithm's parameters produces detection rate up to 0.94. Taking into account abnormal camera location during our tests and therefore geometrical distortion and troubles from trees this result could be considered as passable. Correlation between source data, such as license Plate dimensions and texture, cameras location and others, and parameters of algorithm were also defined.

  14. Mass-producible microscopic computer-generated holograms: microtags

    SciTech Connect

    Descour, M.R.; Sweatt, W.C.; Ray-Chaudhuri, A.K.; Krenz, K.D.; Warren, M.E.; Kravitz, S.H.; Tichenor, D.A.; Stulen, R.H.; Love, T.L.

    1996-12-01

    We have developed a method for encoding phase and amplitude in microscopic computer-generated holograms (microtags) for security applications. An 8{times}8 cell phase-only and an 8{times}8 cell phase-and-amplitude microtag design has been exposed in photoresist by the extreme-ultraviolet (13.4-nm) lithography tool developed at Sandia National Laboratories. Each microtag measures 80 {mu}m{times}160 {mu}m and contains features that are 0.2 {mu}m wide. Fraunhofer zone diffraction patterns can be obtained from fabricated microtags without any intervening optics and compare favorably with predicted diffraction patterns. {copyright} {ital 1996 Optical Society of America.}

  15. Unsteady aerodynamics of fluttering and tumbling plates

    NASA Astrophysics Data System (ADS)

    Andersen, A.; Pesavento, U.; Wang, Z. Jane

    2005-10-01

    We investigate the aerodynamics of freely falling plates in a quasi-two-dimensional flow at Reynolds number of 10(3) , which is typical for a leaf or business card falling in air. We quantify the trajectories experimentally using high-speed digital video at sufficient resolution to determine the instantaneous plate accelerations and thus to deduce the instantaneous fluid forces. We compare the measurements with direct numerical solutions of the two-dimensional Navier Stokes equation. Using inviscid theory as a guide, we decompose the fluid forces into contributions due to acceleration, translation, and rotation of the plate. For both fluttering and tumbling we find that the fluid circulation is dominated by a rotational term proportional to the angular velocity of the plate, as opposed to the translational velocity for a glider with fixed angle of attack. We find that the torque on a freely falling plate is small, i.e. the torque is one to two orders of magnitude smaller than the torque on a glider with fixed angle of attack. Based on these results we revise the existing ODE models of freely falling plates. We get access to different kinds of dynamics by exploring the phase diagram spanned by the Reynolds number, the dimensionless moment of inertia, and the thickness-to-width ratio. In agreement with previous experiments, we find fluttering, tumbling, and apparently chaotic motion. We further investigate the dependence on initial conditions and find brief transients followed by periodic fluttering described by simple harmonics and tumbling with a pronounced period-two structure. Near the cusp-like turning points, the plates elevate, a feature which would be absent if the lift depended on the translational velocity alone.

  16. Nuclear reactor alignment plate configuration

    SciTech Connect

    Altman, David A; Forsyth, David R; Smith, Richard E; Singleton, Norman R

    2014-01-28

    An alignment plate that is attached to a core barrel of a pressurized water reactor and fits within slots within a top plate of a lower core shroud and upper core plate to maintain lateral alignment of the reactor internals. The alignment plate is connected to the core barrel through two vertically-spaced dowel pins that extend from the outside surface of the core barrel through a reinforcement pad and into corresponding holes in the alignment plate. Additionally, threaded fasteners are inserted around the perimeter of the reinforcement pad and into the alignment plate to further secure the alignment plate to the core barrel. A fillet weld also is deposited around the perimeter of the reinforcement pad. To accomodate thermal growth between the alignment plate and the core barrel, a gap is left above, below and at both sides of one of the dowel pins in the alignment plate holes through with the dowel pins pass.

  17. A method for fast automated microscope image stitching.

    PubMed

    Yang, Fan; Deng, Zhen-Sheng; Fan, Qiu-Hong

    2013-05-01

    Image stitching is an important technology to produce a panorama or larger image by combining several images with overlapped areas. In many biomedical researches, image stitching is highly desirable to acquire a panoramic image which represents large areas of certain structures or whole sections, while retaining microscopic resolution. In this study, we develop a fast normal light microscope image stitching algorithm based on feature extraction. At first, an algorithm of scale-space reconstruction of speeded-up robust features (SURF) was proposed to extract features from the images to be stitched with a short time and higher repeatability. Then, the histogram equalization (HE) method was employed to preprocess the images to enhance their contrast for extracting more features. Thirdly, the rough overlapping zones of the images preprocessed were calculated by phase correlation, and the improved SURF was used to extract the image features in the rough overlapping areas. Fourthly, the features were corresponded by matching algorithm and the transformation parameters were estimated, then the images were blended seamlessly. Finally, this procedure was applied to stitch normal light microscope images to verify its validity. Our experimental results demonstrate that the improved SURF algorithm is very robust to viewpoint, illumination, blur, rotation and zoom of the images and our method is able to stitch microscope images automatically with high precision and high speed. Also, the method proposed in this paper is applicable to registration and stitching of common images as well as stitching the microscope images in the field of virtual microscope for the purpose of observing, exchanging, saving, and establishing a database of microscope images.

  18. Investigating Students' Mental Models and Knowledge Construction of Microscopic Friction. I. Implications for Curriculum Design and Development

    ERIC Educational Resources Information Center

    Corpuz, Edgar D.; Rebello, N. Sanjay

    2011-01-01

    In this paper, we discuss the first phase of a multiphase study aimed at investigating the dynamics of students' knowledge construction in the context of unfamiliar physical phenomenon--microscopic friction. The first phase of this study involved the investigation of the variations in students' mental models of microscopic friction. Clinical…

  19. The Plate Tectonics Project

    ERIC Educational Resources Information Center

    Hein, Annamae J.

    2011-01-01

    The Plate Tectonics Project is a multiday, inquiry-based unit that facilitates students as self-motivated learners. Reliable Web sites are offered to assist with lessons, and a summative rubric is used to facilitate the holistic nature of the project. After each topic (parts of the Earth, continental drift, etc.) is covered, the students will…

  20. Unitary plate electrode

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor); Clough, Thomas J. (Inventor); Josefowicz, Jack Y. (Inventor); Sibert, John W. (Inventor)

    1985-01-01

    The unitary electrode (10) comprises a porous sheet (12) of fiberglass the strands (14) of which contain a coating (16) of conductive tin oxide. The lower portion of the sheet contains a layer (18) of resin and the upper layer (20) contains lead dioxide forming a positive active electrode on an electrolyte-impervious layer. The strands (14) form a continuous conduction path through both layers (16, 18). Tin oxide is prevented from reduction by coating the surface of the plate facing the negative electrode with a conductive, impervious layer resistant to reduction such as a thin film (130) of lead or graphite filled resin adhered to the plate with a layer (31) of conductive adhesive. The plate (10) can be formed by casting a molten resin from kettle (60) onto a sheet of glass wool (56) overlying a sheet of lead foil and then applying positive active paste from hopper (64) into the upper layer (68). The plate can also be formed by passing an assembly of a sheet ( 80) of resin, a sheet (86) of sintered glass and a sheet (90) of lead between the nip (92) of heated rollers (93, 95) and then filling lead oxide into the pores (116) of the upper layer (118).

  1. Growth Plate Injuries

    MedlinePlus

    ... or crushed, the growth plate may close prematurely, forming a bony bridge or “bar.” The risk of ... this publication: James S. Panagis, M.D., M.P.H., NIAMS/NIH; R. Tracy Ballock, M.D., Case ...

  2. INL HIP Plate Fabrication

    SciTech Connect

    B. H. Park; C. R. Clark; J. F. Jue

    2010-02-01

    This document outlines the process used to bond monolithic fuel plates by Hot Isostatic Pressing (HIP). This method was developed at Idaho National Laboratory (INL) for the Reduced Enrichment for Research and Test Reactors (RERTR) program. These foils have been used in a number of irradiation experiments in support of the United States Global Threat Reduction Initiative (GTRI) program.

  3. A desktop extreme ultraviolet microscope based on a compact laser-plasma light source

    NASA Astrophysics Data System (ADS)

    Wachulak, P. W.; Torrisi, A.; Bartnik, A.; Węgrzyński, Ł.; Fok, T.; Fiedorowicz, H.

    2017-01-01

    A compact, desktop size microscope, based on laser-plasma source and equipped with reflective condenser and diffractive Fresnel zone plate objective, operating in the extreme ultraviolet (EUV) region at the wavelength of 13.8 nm, was developed. The microscope is capable of capturing magnified images of objects with 95-nm full-pitch spatial resolution (48 nm 25-75% KE) and exposure time as low as a few seconds, combining reasonable acquisition conditions with stand-alone desktop footprint. Such EUV microscope can be regarded as a complementary imaging tool to already existing, well-established ones. Details about the microscope, characterization, resolution estimation and real sample images are presented and discussed.

  4. Field-portable pixel super-resolution colour microscope.

    PubMed

    Greenbaum, Alon; Akbari, Najva; Feizi, Alborz; Luo, Wei; Ozcan, Aydogan

    2013-01-01

    Based on partially-coherent digital in-line holography, we report a field-portable microscope that can render lensfree colour images over a wide field-of-view of e.g., >20 mm(2). This computational holographic microscope weighs less than 145 grams with dimensions smaller than 17×6×5 cm, making it especially suitable for field settings and point-of-care use. In this lensfree imaging design, we merged a colorization algorithm with a source shifting based multi-height pixel super-resolution technique to mitigate 'rainbow' like colour artefacts that are typical in holographic imaging. This image processing scheme is based on transforming the colour components of an RGB image into YUV colour space, which separates colour information from brightness component of an image. The resolution of our super-resolution colour microscope was characterized using a USAF test chart to confirm sub-micron spatial resolution, even for reconstructions that employ multi-height phase recovery to handle dense and connected objects. To further demonstrate the performance of this colour microscope Papanicolaou (Pap) smears were also successfully imaged. This field-portable and wide-field computational colour microscope could be useful for tele-medicine applications in resource poor settings.

  5. Field-Portable Pixel Super-Resolution Colour Microscope

    PubMed Central

    Greenbaum, Alon; Akbari, Najva; Feizi, Alborz; Luo, Wei; Ozcan, Aydogan

    2013-01-01

    Based on partially-coherent digital in-line holography, we report a field-portable microscope that can render lensfree colour images over a wide field-of-view of e.g., >20 mm2. This computational holographic microscope weighs less than 145 grams with dimensions smaller than 17×6×5 cm, making it especially suitable for field settings and point-of-care use. In this lensfree imaging design, we merged a colorization algorithm with a source shifting based multi-height pixel super-resolution technique to mitigate ‘rainbow’ like colour artefacts that are typical in holographic imaging. This image processing scheme is based on transforming the colour components of an RGB image into YUV colour space, which separates colour information from brightness component of an image. The resolution of our super-resolution colour microscope was characterized using a USAF test chart to confirm sub-micron spatial resolution, even for reconstructions that employ multi-height phase recovery to handle dense and connected objects. To further demonstrate the performance of this colour microscope Papanicolaou (Pap) smears were also successfully imaged. This field-portable and wide-field computational colour microscope could be useful for tele-medicine applications in resource poor settings. PMID:24086742

  6. Solid-state optical microscope

    DOEpatents

    Young, I.T.

    1981-01-07

    A solid state optical microscope is described wherein wide-field and high-resolution images of an object are produced at a rapid rate by utilizing conventional optics with a charge-coupled photodiode array. Means for scanning in one of two orthogonal directions are provided, while the charge-coupled photodiode array scans in the other orthogonal direction. Illumination light from the object is incident upon the photodiodes, creating packets of electrons (signals) which are representative of the illuminated object. The signals are then processed, stored in a memory, and finally displayed as a video signal.

  7. Stimulated Parametric Emission Microscope Systems

    NASA Astrophysics Data System (ADS)

    Itoh, Kazuyoshi; Isobe, Keisuke

    2006-10-01

    We present a novel microscopy technique based on the fourwave mixing (FWM) process that is enhanced by two-photon electronic resonance induced by a pump pulse along with stimulated emission induced by a dump pulse. A Ti:sapphire laser and an optical parametric oscillator are used as light sources for the pump and dump pulses, respectively. We demonstrate that our FWM technique can be used to obtain two-dimensional microscopic images of an unstained leaf of Camellia sinensis and an unlabeled tobacco BY2 Cell.

  8. Stimulated Brillouin Scattering Microscopic Imaging

    PubMed Central

    Ballmann, Charles W.; Thompson, Jonathan V.; Traverso, Andrew J.; Meng, Zhaokai; Scully, Marlan O.; Yakovlev, Vladislav V.

    2015-01-01

    Two-dimensional stimulated Brillouin scattering microscopy is demonstrated for the first time using low power continuous-wave lasers tunable around 780 nm. Spontaneous Brillouin spectroscopy has much potential for probing viscoelastic properties remotely and non-invasively on a microscopic scale. Nonlinear Brillouin scattering spectroscopy and microscopy may provide a way to tremendously accelerate the data aquisition and improve spatial resolution. This general imaging setup can be easily adapted for specific applications in biology and material science. The low power and optical wavelengths in the water transparency window used in this setup provide a powerful bioimaging technique for probing the mechanical properties of hard and soft tissue. PMID:26691398

  9. Microscopic Analysis of Activated Sludge. Training Manual.

    ERIC Educational Resources Information Center

    Office of Water Program Operations (EPA), Cincinnati, OH. National Training and Operational Technology Center.

    This training manual presents material on the use of a compound microscope to analyze microscope communities, present in wastewater treatment processes, for operational control. Course topics include: sampling techniques, sample handling, laboratory analysis, identification of organisms, data interpretation, and use of the compound microscope.…

  10. A Student-Built Scanning Tunneling Microscope

    ERIC Educational Resources Information Center

    Ekkens, Tom

    2015-01-01

    Many introductory and nanotechnology textbooks discuss the operation of various microscopes including atomic force (AFM), scanning tunneling (STM), and scanning electron microscopes (SEM). In a nanotechnology laboratory class, students frequently utilize microscopes to obtain data without a thought about the detailed operation of the tool itself.…

  11. Inspection and characterization of flexo-printing plates

    NASA Astrophysics Data System (ADS)

    Hahlweg, Cornelius; Pescoller, Lukas; Zhao, Wenjing

    2013-09-01

    In continuation of last year's paper on distorting optics for inspection of 2 1/2D surfaces with convex or elevated elements - like braille paper and other special printing products - the present paper is dedicated to the quality control and characterization of flexo-printing plates. The need for high optical resolution contradicts the need for depth of field. A rugged optical system for gathering a series of microscopic images at various planes of focus is discussed.

  12. Registration of multiple image sets with thin-plate spline

    NASA Astrophysics Data System (ADS)

    He, Liang; Houk, James C.

    1994-09-01

    A thin-plate spline method for spatial warping was used to register multiple image sets during 3D reconstruction of histological sections. In a neuroanatomical study, the same labeling method was applied to several turtle brains. Each case produced a set of microscopic sections. Spatial warping was employed to map data sets from multiple cases onto a template coordinate system. This technique enabled us to produce an anatomical reconstruction of a neural network that controls limb movement.

  13. Inspection with Robotic Microscopic Imaging

    NASA Technical Reports Server (NTRS)

    Pedersen, Liam; Deans, Matthew; Kunz, Clay; Sargent, Randy; Chen, Alan; Mungas, Greg

    2005-01-01

    Future Mars rover missions will require more advanced onboard autonomy for increased scientific productivity and reduced mission operations cost. One such form of autonomy can be achieved by targeting precise science measurements to be made in a single command uplink cycle. In this paper we present an overview of our solution to the subproblems of navigating a rover into place for microscopic imaging, mapping an instrument target point selected by an operator using far away science camera images to close up hazard camera images, verifying the safety of placing a contact instrument on a sample or finding nearby safe points, and analyzing the data that comes back from the rover. The system developed includes portions used in the Multiple Target Single Cycle Instrument Placement demonstration at NASA Ames in October 2004, and portions of the MI Toolkit delivered to the Athena Microscopic Imager Instrument Team for the MER mission still operating on Mars today. Some of the component technologies are also under consideration for MSL mission infusion.

  14. Sensing mode atomic force microscope

    DOEpatents

    Hough, Paul V. C.; Wang, Chengpu

    2006-08-22

    An atomic force microscope is described having a cantilever comprising a base and a probe tip on an end opposite the base; a cantilever drive device connected to the base; a magnetic material coupled to the probe tip, such that when an incrementally increasing magnetic field is applied to the magnetic material an incrementally increasing force will be applied to the probe tip; a moveable specimen base; and a controller constructed to obtain a profile height of a specimen at a point based upon a contact between the probe tip and a specimen, and measure an adhesion force between the probe tip and the specimen by, under control of a program, incrementally increasing an amount of a magnetic field until a release force, sufficient to break the contact, is applied. An imaging method for atomic force microscopy involving measuring a specimen profile height and adhesion force at multiple points within an area and concurrently displaying the profile and adhesion force for each of the points is also described. A microscope controller is also described and is constructed to, for a group of points, calculate a specimen height at a point based upon a cantilever deflection, a cantilever base position and a specimen piezo position; calculate an adhesion force between a probe tip and a specimen at the point by causing an incrementally increasing force to be applied to the probe tip until the probe tip separates from a specimen; and move the probe tip to a new point in the group.

  15. Sensing mode atomic force microscope

    DOEpatents

    Hough, Paul V.; Wang, Chengpu

    2004-11-16

    An atomic force microscope is described having a cantilever comprising a base and a probe tip on an end opposite the base; a cantilever drive device connected to the base; a magnetic material coupled to the probe tip, such that when an incrementally increasing magnetic field is applied to the magnetic material an incrementally increasing force will be applied to the probe tip; a moveable specimen base; and a controller constructed to obtain a profile height of a specimen at a point based upon a contact between the probe tip and a specimen, and measure an adhesion force between the probe tip and the specimen by, under control of a program, incrementally increasing an amount of a magnetic field until a release force, sufficient to break the contact, is applied. An imaging method for atomic force microscopy involving measuring a specimen profile height and adhesion force at multiple points within an area and concurrently displaying the profile and adhesion force for each of the points is also described. A microscope controller is also described and is constructed to, for a group of points, calculate a specimen height at a point based upon a cantilever deflection, a cantilever base position and a specimen piezo position; calculate an adhesion force between a probe tip and a specimen at the point by causing an incrementally increasing force to be applied to the probe tip until the probe tip separates from a specimen; and move the probe tip to a new point in the group.

  16. Sensing mode atomic force microscope

    DOEpatents

    Hough, Paul V. C.; Wang, Chengpu

    2003-01-01

    An atomic force microscope utilizes a pulse release system and improved method of operation to minimize contact forces between a probe tip affixed to a flexible cantilever and a specimen being measured. The pulse release system includes a magnetic particle affixed proximate the probe tip and an electromagnetic coil. When energized, the electromagnetic coil generates a magnetic field which applies a driving force on the magnetic particle sufficient to overcome adhesive forces exhibited between the probe tip and specimen. The atomic force microscope includes two independently displaceable piezo elements operable along a Z-axis. A controller drives the first Z-axis piezo element to provide a controlled approach between the probe tip and specimen up to a point of contact between the probe tip and specimen. The controller then drives the first Z-axis piezo element to withdraw the cantilever from the specimen. The controller also activates the pulse release system which drives the probe tip away from the specimen during withdrawal. Following withdrawal, the controller adjusts the height of the second Z-axis piezo element to maintain a substantially constant approach distance between successive samples.

  17. Martian Magnets Under the Microscope

    NASA Technical Reports Server (NTRS)

    2004-01-01

    NASA's Mars Exploration Rover Spirit acquired this microscopic imager view of its capture magnet on sol 92 (April 6, 2004). Both Spirit and the Mars Exploration Rover Opportunity are equipped with a number of magnets. The capture magnet, as seen here, has a stronger charge than its sidekick, the filter magnet. The lower-powered filter magnet captures only the most magnetic airborne dust with the strongest charges, while the capture magnet picks up all magnetic airborne dust.

    The magnets' primary purpose is to collect the martian magnetic dust so that scientists can analyze it with the rovers' Moessbauer spectrometers. While there is plenty of dust on the surface of Mars, it is difficult to confirm where it came from, and when it was last airborne. Because scientists are interested in learning about the properties of the dust in the atmosphere, they devised this dust-collection experiment.

    The capture magnet is about 4.5 centimeters (1.8 inches) in diameter and is constructed with a central cylinder and three rings, each with alternating orientations of magnetization. Scientists have been monitoring the continual accumulation of dust since the beginning of the mission with panoramic camera and microscopic imager images. They had to wait until enough dust accumulated before they could get a Moessbauer spectrometer analysis. The results of that analysis, performed on sol 92, have not been sent back to Earth yet.

  18. On electrostatically actuated NEMS/MEMS circular plates

    NASA Astrophysics Data System (ADS)

    Caruntu, Dumitru I.; Alvarado, Iris

    2011-04-01

    This paper deals with electrostatically actuated micro and nano-electromechanical (MEMS/NEMS) circular plates. The system under investigation consists of two bodies, a deformable and conductive circular plate placed above a fixed, rigid and conductive ground plate. The deformable circular plate is electrostatically actuated by applying an AC voltage between the two plates. Nonlinear parametric resonance and pull-in occur at certain frequencies and relatively large AC voltage, respectively. Such phenomena are useful for applications such as sensors, actuators, switches, micro-pumps, micro-tweezers, chemical and mass sensing, and micro-mirrors. A mathematical model of clamped circular MEMS/NEMS electrostatically actuated plates has been developed. Since the model is in the micro- and nano-scale, surface forces, van der Waals and/or Casimir, acting on the plate are included. A perturbation method, the Method of Multiple Scales (MMS), is used for investigating the case of weakly nonlinear MEMS/NEMS circular plates. Two time scales, fast and slow, are considered in this work. The amplitude-frequency and phase-frequency response of the plate in the case of primary resonance are obtained and discussed.

  19. High-speed imaging of Raleigh-Taylor instabilities in laser-driven plates

    NASA Astrophysics Data System (ADS)

    Frank, Alan M.; Gillespie, Calvin H.; Trott, Wayne M.

    1997-05-01

    We have previously reported our observations of the dynamic behavior of laser driven plates. Recent improvements and modification of the imaging techniques have identified and provided measurements of Raleigh-Taylor (R-T) instabilities that occur in these events. The microscope system in the LLNL Micro Detonics Facility, was converted to an epi- illuminated polarization configuration. A double pulse nanosecond illuminator and a second independently focusable frame camera were also added to the system. A laser driven plate, that is a dense solid driven by a laser heated, lower density plasma, is inherently R-T unstable. The characteristics and growth of the instability determine whether or not the plate remains intact. In earlier reports we correlated the surface patterning of thin plates with the fiber-optical transmission modes. In subsequent experiments we noted that the plasma burn through patterning in thin plates and the surface patterning of thicker plates did not correspond to the thin plate early time patterning. These observations led to the suspicion of R-T instability. A series of experiments correlating plate thickness and pattern spatial frequency has verified the instability. The plates are aluminum, deposited on the ends of optical fibers. They are launched by a YAG laser pulse traveling down the fiber. Plate velocities are several kilometers per second and characteristic dimensions of the instabilities are a few to tens of microns. Several techniques were used to examine the plates, the most successful being specularly reflecting polarization microscopy looking directly at the plate as it flies toward the camera. These images gave data on the spatial frequencies of the instabilities but could not give the amplitudes. To measure the amplitude of the instability a semi-transparent witness plate was placed a known distance from the plate. As above, the plate was observed using the polarization microscope but using the streak camera as the detector

  20. What Are Growth Plate Injuries?

    MedlinePlus

    ... nih.gov November 2014 What Are Growth Plate Injuries? Fast Facts: An Easy-to-Read Series of Publications ... Some inherited disorders 1 What Are Growth Plate Injuries? Fast Facts: An Easy-to-Read Series of Publications ...