Evaluation of Alternative Splicing Regulators As Targets For Selective Therapy of Triple Negative (Basal) Breast Carcinoma

DTIC Science & Technology

2016-10-01

KHDRBS3 and SRSF12 on tumor progression and metastasis (Task1). We analyzed the effect of KHDRBS3 depletion on the growth and migration properties of the...tumor growth during the second year of this project. Continued analysis of the splicing factor expression in primary tumor samples further supports...depletion on tumor initiation, growth and metastasis. Keywords Pre-mRNA splicing, breast cancer, KHDRBS3, SRPK1, SRSF12, metastasis

Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River—Annual report 2014

USGS Publications Warehouse

Delonay, Aaron J.; Chojnacki, Kimberly A.; Jacobson, Robert B.; Braaten, Patrick J.; Buhl, Kevin J.; Elliott, Caroline M.; Erwin, Susannah O.; Faulkner, Jacob D.A.; Candrl, James S.; Fuller, David B.; Backes, Kenneth M.; Haddix, Tyler M.; Rugg, Matthew L.; Wesolek, Christopher J.; Eder, Brandon L.; Mestl, Gerald E.

2016-03-16

The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with collaborating research partners and in cooperation with the U.S. Army Corps of Engineers, Missouri River Recovery Program–Integrated Science Program. The project research consists of several interdependent and complementary tasks that involve multiple disciplines.The project research tasks in the 2014 scope of work emphasized understanding of reproductive migrations and spawning of adult pallid sturgeon and hatch and drift of larvae. These tasks were addressed in three hydrologically and geomorphologically distinct parts of the Missouri River Basin: the Lower Missouri River downstream from Gavins Point Dam, the Upper Missouri River downstream from Fort Peck Dam and downstream reaches of the Milk River, and the Lower Yellowstone River. The project research is designed to inform management decisions related to channel re-engineering, flow modification, and pallid sturgeon population augmentation on the Missouri River and throughout the range of the species. Research and progress made through this project are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by the Comprehensive Sturgeon Research Project during 2014.

Webinar on PTM with CMS

DTIC Science & Technology

2013-12-04

Coral Reef Dredging Project SAV Migrating Fish Coral Reef Dredging Project SAV Migrating Fish... Coral Reef Dredging Project SAV Migrating Fish Coral Reef Dredging Project Coastal and Hydraulics Laboratory 22 Dredging Materials and...Introduction to CMS Coastal and Hydraulics Laboratory Integrated waves , current, and sediment transport model in the Surface-water Modeling

Bayesian Probabilistic Projection of International Migration.

PubMed

Azose, Jonathan J; Raftery, Adrian E

2015-10-01

We propose a method for obtaining joint probabilistic projections of migration for all countries, broken down by age and sex. Joint trajectories for all countries are constrained to satisfy the requirement of zero global net migration. We evaluate our model using out-of-sample validation and compare point projections to the projected migration rates from a persistence model similar to the method used in the United Nations' World Population Prospects, and also to a state-of-the-art gravity model.

Landbird migration in the American West: Recent progress and future research directions

USGS Publications Warehouse

Carlisle, J.D.; Skagen, S.K.; Kus, B.E.; van Riper, Charles; Paxton, K.L.; Kelly, J.F.

2009-01-01

Our knowledge of avian behaviors during the nonbreeding period still lags behind that of the breeding season, but the last decade has witnessed a proliferation in research that has yielded significant progress in understanding migration patterns of North American birds. And, although historically the great majority of migration research has been conducted in the eastern half of the continent, there has been much recent progress on aspects of avian migration in the West. In particular, expanded use of techniques such as radar, plasma metabolites, mist-netting, count surveys, stable isotopes, genetic data, and animal tracking, coupled with an increase in multi-investigator collaborations, have all contributed to this growth of knowledge. There is increasing recognition that migration is likely the most limiting time of year for migratory birds, increasing the importance of continuing to decipher patterns of stopover ecology, identifying critical stopover habitats, and documenting migration routes in the diverse and changing landscapes of the American West. Here, we review and briefly synthesize the latest findings and advances in avian migration and consider research needs to guide future research on migration in the West. ?? 2009 by The Cooper Ornithological Society. All rights reserved.

Progress of the Enhanced Hanford Single Shell Tank (SST) Integrity Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Venetz, Theodore J.; Washenfelder, Dennis J.; Boomer, Kayle D.

2015-01-07

To improve the understanding of the single-shell tanks (SSTs) integrity, Washington River Protection Solutions, LLC (WRPS), the USDOE Hanford Site tank contractor, developed an enhanced Single-Shell Tank Integrity Project (SSTIP) in 2009. An expert panel on SST integrity, consisting of various subject matters experts in industry and academia, was created to provide recommendations supporting the development of the project. This panel developed 33 recommendations in four main areas of interest: structural integrity, liner degradation, leak integrity and prevention, and mitigation of contamination migration. In late 2010, seventeen of these recommendations were used to develop the basis for the M-45-10-1 Changemore » Package for the Hanford Federal Agreement and Compliance Order, which is also known as the Tri-Party Agreement.« less

CML/CD36 accelerates atherosclerotic progression via inhibiting foam cell migration.

PubMed

Xu, Suining; Li, Lihua; Yan, Jinchuan; Ye, Fei; Shao, Chen; Sun, Zhen; Bao, Zhengyang; Dai, Zhiyin; Zhu, Jie; Jing, Lele; Wang, Zhongqun

2018-01-01

Among the various complications of type 2 diabetes mellitus, atherosclerosis causes the highest disability and morbidity. A multitude of macrophage-derived foam cells are retained in atherosclerotic plaques resulting not only from recruitment of monocytes into lesions but also from a reduced rate of macrophage migration from lesions. Nε-carboxymethyl-Lysine (CML), an advanced glycation end product, is responsible for most complications of diabetes. This study was designed to investigate the mechanism of CML/CD36 accelerating atherosclerotic progression via inhibiting foam cell migration. In vivo study and in vitro study were performed. For the in vivo investigation, CML/CD36 accelerated atherosclerotic progression via promoting the accumulation of macrophage-derived foam cells in aorta and inhibited macrophage-derived foam cells in aorta migrating to the para-aorta lymph node of diabetic apoE -/- mice. For the in vitro investigation, CML/CD36 inhibited RAW264.7-derived foam cell migration through NOX-derived ROS, FAK phosphorylation, Arp2/3 complex activation and F-actin polymerization. Thus, we concluded that CML/CD36 inhibited foam cells of plaque migrating to para-aorta lymph nodes, accelerating atherosclerotic progression. The corresponding mechanism may be via free cholesterol, ROS generation, p-FAK, Arp2/3, F-actin polymerization. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Does Specification Matter? Experiments with Simple Multiregional Probabilistic Population Projections

PubMed Central

Raymer, James; Abel, Guy J.; Rogers, Andrei

2012-01-01

Population projection models that introduce uncertainty are a growing subset of projection models in general. In this paper, we focus on the importance of decisions made with regard to the model specifications adopted. We compare the forecasts and prediction intervals associated with four simple regional population projection models: an overall growth rate model, a component model with net migration, a component model with in-migration and out-migration rates, and a multiregional model with destination-specific out-migration rates. Vector autoregressive models are used to forecast future rates of growth, birth, death, net migration, in-migration and out-migration, and destination-specific out-migration for the North, Midlands and South regions in England. They are also used to forecast different international migration measures. The base data represent a time series of annual data provided by the Office for National Statistics from 1976 to 2008. The results illustrate how both the forecasted subpopulation totals and the corresponding prediction intervals differ for the multiregional model in comparison to other simpler models, as well as for different assumptions about international migration. The paper ends end with a discussion of our results and possible directions for future research. PMID:23236221

Expanding Applications of the Nano Intravital Device as a Platform for Exploring Tumor Microenvironments

NASA Astrophysics Data System (ADS)

Padgen, Michael R.

The tumor microenvironment has been demonstrated to be a key determinant in the progression of cancer. Unfortunately, the mechanisms behind the different microenvironments (cytokine gradients, hypoxia, hypoglycemia, etc) have not been fully elucidated. Identifying these mechanisms can lead to targeted, individualized therapy to prevent metastasis. The Nano Intravital Device (NANIVID) is a microfabricated, implantable device designed to initiate specific microenvironments in vivo so that the time course of the effects can be observed. With both spatial and temporal control over the induced environments, the affected regions of the tumor can be compared to the rest of the tumor. The NANIVID was first used to establish cytokine gradients to monitor the migration of invasive cancer cells. The three projects that comprise this work expand the applications of the NANIVID to establish the device as a robust platform for investigating tumor microenvironment interactions. The first project released chemical mimics from the device to induce the cellular hypoxic response in tumors to determine how hypoxia affects the fate of disseminated tumor cells. The second project used the NANIVID in combination with an atomic force microscope to investigate the altered mechanics of migrating invasive cancer cells. The final project was to develop a cell counter to monitor the isolation of the invasive subpopulation of cells that were drawn into the device using a chemoattractant. These three projects demonstrate the potential of the NANIVID as a platform for investigating the tumor microenvironment.

LHCb migration from Subversion to Git

NASA Astrophysics Data System (ADS)

Clemencic, M.; Couturier, B.; Closier, J.; Cattaneo, M.

2017-10-01

Due to user demand and to support new development workflows based on code review and multiple development streams, LHCb decided to port the source code management from Subversion to Git, using the CERN GitLab hosting service. Although tools exist for this kind of migration, LHCb specificities and development models required careful planning of the migration, development of migration tools, changes to the development model, and redefinition of the release procedures. Moreover we had to support a hybrid situation with some software projects hosted in Git and others still in Subversion, or even branches of one projects hosted in different systems. We present the way we addressed the special LHCb requirements, the technical details of migrating large non standard Subversion repositories, and how we managed to smoothly migrate the software projects following the schedule of each project manager.

Migrating Legacy Systems in the Global Merger & Acquisition Environment

ERIC Educational Resources Information Center

Katerattanakul, Pairin; Kam, Hwee-Joo; Lee, James J.; Hong, Soongoo

2009-01-01

The MetaFrame system migration project at WorldPharma, while driven by merger and acquisition, had faced complexities caused by both technical challenges and organizational issues in the climate of uncertainties. However, WorldPharma still insisted on instigating this post-merger system migration project. This project served to (1) consolidate the…

Leaving A Legacy: Parental Migration and School Outcomes Among Young Children in the Philippines

PubMed Central

Asis, Maruja M.B.; Ruiz-Marave, Cecilia

2014-01-01

This article examines the link between parental migration and young children’s education using data from the Philippine country study of the Child Health and Migrant Parents in South-East Asia (CHAMPSEA) Project. The key research question probed here is: what difference does parental migration make to the school outcomes of young children? Specifically, it looks at factors that explain children’s school progression (school pacing) and academic performance (school achievement) using multiple regression analysis. These questions are explored using CHAMPSEA data gathered from a survey of children under 12 years of age and their households in Laguna and Batangas (n=487). The concern that parental absence due to migration can negatively affect the school performance of children is not supported by the study. If parental migration affects school outcomes, it is associated with positive outcomes, or with outcomes which show that children in transnational households are not doing worse than children living with both parents. Positive school outcomes are best associated with a migrant-carer arrangement where fathers work abroad and mothers stay home as carers –children in these households fare very well when it comes to school pacing and school achievement. The study concludes that families and households need to provide both economic and psychological support to enhance the chances that children are at pace with their schooling and are doing well at school. PMID:24954962

Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River: annual report 2011

USGS Publications Warehouse

DeLonay, Aaron J.; Jacobson, Robert B.; Chojnacki, Kimberly A.; Annis, Mandy L.; Braaten, P. J.; Elliott, Caroline M.; Fuller, D. B.; Haas, Justin D.; Haddix, Tyler M.; Ladd, Hallie L.A.; McElroy, Brandon J.; Mestl, Gerald E.; Papoulias, Diana M.; Rhoten, Jason C.; Wildhaber, Mark L.

2014-01-01

The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with cooperating research partners and in collaboration with the U.S. Army Corps of Engineers, Missouri River Recovery—Integrated Science Program. The research consists of several interdependent and complementary tasks that engage multiple disciplines. The research tasks in the 2011 scope of work emphasized understanding of reproductive migrations and spawning of adult sturgeon, and hatch and drift of larvae. These tasks were addressed in three hydrologically and geomorphologically distinct parts of the Missouri River Basin: the Lower Missouri River downstream from Gavins Point Dam, the Upper Missouri River downstream from Fort Peck Dam and including downstream reaches of the Milk River, and the Lower Yellowstone River. The research is designed to inform management decisions related to channel re-engineering, flow modification, and pallid sturgeon population augmentation on the Missouri River, and throughout the range of the species. Research and progress made through this project are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by the Comprehensive Sturgeon Research Project during 2011.

Mitigating artifacts in back-projection source imaging with implications for frequency-dependent properties of the Tohoku-Oki earthquake

NASA Astrophysics Data System (ADS)

Meng, Lingsen; Ampuero, Jean-Paul; Luo, Yingdi; Wu, Wenbo; Ni, Sidao

2012-12-01

Comparing teleseismic array back-projection source images of the 2011 Tohoku-Oki earthquake with results from static and kinematic finite source inversions has revealed little overlap between the regions of high- and low-frequency slip. Motivated by this interesting observation, back-projection studies extended to intermediate frequencies, down to about 0.1 Hz, have suggested that a progressive transition of rupture properties as a function of frequency is observable. Here, by adapting the concept of array response function to non-stationary signals, we demonstrate that the "swimming artifact", a systematic drift resulting from signal non-stationarity, induces significant bias on beamforming back-projection at low frequencies. We introduce a "reference window strategy" into the multitaper-MUSIC back-projection technique and significantly mitigate the "swimming artifact" at high frequencies (1 s to 4 s). At lower frequencies, this modification yields notable, but significantly smaller, artifacts than time-domain stacking. We perform extensive synthetic tests that include a 3D regional velocity model for Japan. We analyze the recordings of the Tohoku-Oki earthquake at the USArray and at the European array at periods from 1 s to 16 s. The migration of the source location as a function of period, regardless of the back-projection methods, has characteristics that are consistent with the expected effect of the "swimming artifact". In particular, the apparent up-dip migration as a function of frequency obtained with the USArray can be explained by the "swimming artifact". This indicates that the most substantial frequency-dependence of the Tohoku-Oki earthquake source occurs at periods longer than 16 s. Thus, low-frequency back-projection needs to be further tested and validated in order to contribute to the characterization of frequency-dependent rupture properties.

Disentangling fathers’ absences from household remittances in international migration: The case of educational attainment in Guatemala

PubMed Central

Davis, Jason; Brazil, Noli

2016-01-01

Estimating the effects of international migration on left-behind children’s educational attainment is complicated by the potential offsetting effects of fathers’ absences and household remittances. Most research has not separated these aspects of international migration on children’s human capital outcomes. We address this deficiency by using instrumental variables to isolate the effects of fathers’ international migration absences from international household remittances on student enrollment and grade progression in Guatemala. Results indicate that fathers’ absences and household international remittances are negatively related to enrollment, providing evidence for a culture of migration effect. For students who remain in school, household international remittances neutralize the harmful influence of fathers’ absences on grade progression. PMID:27293309

Multigeneration data migration from legacy systems

NASA Astrophysics Data System (ADS)

Ratib, Osman M.; Liu, Brent J.; Kho, Hwa T.; Tao, Wenchao; Wang, Cun; McCoy, J. Michael

2003-05-01

The migration of image data from different generations of legacy archive systems represents a technical challenge and in incremental cost in transitions to newer generations of PACS. UCLA medical center has elected to completely replace the existing PACS infrastructure encompassing several generations of legacy systems by a new commercial system providing enterprise-wide image management and communication. One of the most challenging parts of the project was the migration of large volumes of legacy images into the new system. Planning of the migration required the development of specialized software and hardware, and included different phases of data mediation from existing databases to the new PACS database prior to the migration of the image data. The project plan included a detailed analysis of resources and cost of data migration to optimize the process and minimize the delay of a hybrid operation where the legacy systems need to remain operational. Our analysis and project planning showed that the data migration represents the most critical path in the process of PACS renewal. Careful planning and optimization of the project timeline and resources allocated is critical to minimize the financial impact and the time delays that such migrations can impose on the implementation plan.

Integrated Library System (ILS) Challenges and Opportunities: A Survey of U.S. Academic Libraries with Migration Projects

ERIC Educational Resources Information Center

Wang, Zhonghong

2009-01-01

An online survey was sent to academic libraries and consortia with an integrated library system (ILS) migration project, based on review of press releases from major U.S. ILS vendors. This study takes a systematic approach to provide a snapshot of the academic ILS market and key factors affecting the outcome of an ILS migration project. It reveals…

Differential regulation of microtubule severing by APC underlies distinct patterns of projection neuron and interneuron migration

PubMed Central

Eom, Tae-Yeon; Stanco, Amelia; Guo, Jiami; Wilkins, Gary; Deslauriers, Danielle; Yan, Jessica; Monckton, Chase; Blair, Josh; Oon, Eesim; Perez, Abby; Salas, Eduardo; Oh, Adrianna; Ghukasyan, Vladimir; Snider, William D.; Rubenstein, John L. R.; Anton, E. S.

2014-01-01

Coordinated migration of distinct classes of neurons to appropriate positions leads to the formation of functional neuronal circuitry in the cerebral cortex. Two major classes of cortical neurons, interneurons and projection neurons, utilize distinctly different modes (radial vs. tangential) and routes of migration to arrive at their final positions in the cerebral cortex. Here, we show that adenomatous polyposis coli (APC) modulates microtubule (MT) severing in interneurons to facilitate tangential mode of interneuron migration, but not the glial-guided, radial migration of projection neurons. APC regulates the stability and activity of the MT severing protein p60-katanin in interneurons to promote the rapid remodeling of neuronal processes necessary for interneuron migration. These findings reveal how severing and restructuring of MTs facilitate distinct modes of neuronal migration necessary for laminar organization of neurons in the developing cerebral cortex. PMID:25535916

Minneapolis Multi-Ethnic Curriculum Project--Migration Unit.

ERIC Educational Resources Information Center

Minneapolis Public Schools, Minn. Dept. of Intergroup Education.

The student booklet presents short chapters illustrating the migration unit of the Minneapolis Multi-Ethnic Curriculum Project for secondary schools. Sixteen brief chapters describe migration, immigration, and emigration in the United States. The first six chapters offer first person accounts of immigrants from Norway, Korea, Egypt, Hitler's…

Rural migration in Nevada: Lincoln County. Phase 1, 1992--1993

DOE Office of Scientific and Technical Information (OSTI.GOV)

Soden, D.L.; Carns, D.E.; Mosser, D.

1993-12-31

The principal objective of this project was to develop insight into the scope of migration of working age Nevadans out of their county of birth; including the collection of data on their skill levels, desire to out or in-migrate, interactions between families of migratory persons, and the impact that the proposed high-level nuclear waste repository at Yucca mountain might have on their individual, and collective, decisions to migrate and return. The initial phase of this project reported here was conducted in 1992 and 1993 in Lincoln County, Nevada, one of the counties designated as ``affected`` by the proposed repository program.more » The findings suggest that a serious out-migration problem exists in Lincoln County, and that the Yucca mountain project will likely affect decisions relating to migration patterns in the future.« less

Activated Retinal Pigment Epithelium, an Optical Coherence Tomography Biomarker for Progression in Age-Related Macular Degeneration

PubMed Central

Curcio, Christine A.; Zanzottera, Emma C.; Ach, Thomas; Balaratnasingam, Chandrakumar; Freund, K. Bailey

2017-01-01

Purpose To summarize and contextualize recent histology and clinical imaging publications on retinal pigment epithelium (RPE) fate in advanced age-related macular degeneration (AMD); to support RPE activation and migration as important precursors to atrophy, manifest as intraretinal hyperreflective foci in spectral-domain optical coherence tomography (SDOCT). Methods The Project MACULA online resource for AMD histopathology was surveyed systematically to form a catalog of 15 phenotypes of RPE and RPE-derived cells and layer thicknesses in advanced disease. Phenotypes were also sought in correlations with clinical longitudinal eye-tracked SDOCT and with ex vivo imaging–histopathology correlations in geographic atrophy (GA) and pigment epithelium detachments (PED). Results The morphology catalog suggested two main pathways of RPE fate: basolateral shedding of intracellular organelles (apparent apoptosis in situ) and activation with anterior migration. Acquired vitelliform lesions may represent a third pathway. Migrated cells are packed with RPE organelles and confirmed as hyperreflective on SDOCT. RPE layer thickening due to cellular dysmorphia and thick basal laminar deposit is observed near the border of GA. Drusenoid PED show a life cycle of slow growth and rapid collapse preceded by RPE layer disruption and anterior migration. Conclusions RPE activation and migration comprise an important precursor to atrophy that can be observed at the cellular level in vivo via validated SDOCT. Collapse of large drusen and drusenoid PED appears to occur when RPE death and migration prevent continued production of druse components. Data implicate excessive diffusion distance from choriocapillaris in RPE death as well as support a potential benefit in targeting drusen in GA. PMID:28785769

Progress report for project modeling Arctic barrier island-lagoon system response to projected Arctic warming

USGS Publications Warehouse

Erikson, Li H.; Gibbs, Ann E.; Richmond, Bruce M.; Storlazzi, Curt; B.M. Jones,

2012-01-01

Changes in Arctic coastal ecosystems in response to global warming may be some of the most severe on the planet. A better understanding and analysis of the rates at which these changes are expected to occur over the coming decades is crucial in order to delineate high-priority areas that are likely to be affected by climate changes. In this study we investigate the likelihood of changes to habitat-supporting barrier island – lagoon systems in response to projected changes in atmospheric and oceanographic forcing associated with Arctic warming. To better understand the relative importance of processes responsible for the current and future coastal landscape, key parameters related to increasing arctic temperatures are investigated and used to establish boundary conditions for models that simulate barrier island migration and inundation of deltaic deposits and low-lying tundra. The modeling effort investigates the dominance and relative importance of physical processes shaping the modern Arctic coastline as well as decadal responses due to projected conditions out to the year 2100.

Compilation of annual reports of the Navy ELF (Extremely Low Frequency) communications system ecological monitoring program. Volume 1: Tabs A-E

NASA Astrophysics Data System (ADS)

Anderson, M.; Bruhn, J.; Cattelino, P.; Janke, R.; Jurgensen, M.; Mroz, G.; Reed, E. J.; Trettin, C.

1984-07-01

A long-term program of studying ELF electromagnetic influences on ecosystems in northwestern Wisconsin and the Upper Peninsula of Michigan is being conducted. Selection of study sites, monitoring protocols, and analytical methods were initiated in 1982. Data collection was initiated in 1983. Progress is described for studying the terrestrial, aquatic, and wetland ecosystems for the 10 projects comprising the ecological monitoring program. The 10 projects contain Herbaceous Plant Cover and Tree Studies; Litter Decomposition and Microflora; The Effects of Exposing the Slime Mold Physarum polycephalum; Soil Amoeba; Soil and Litter Arthropoda and Earthworm Studies; Biological Studies on Pollinating Insects (Megachilid Bees); Small Vertebrates (Small Mammals and Nesting Birds); Aquatic Ecosystems; Wetland Studies; and Field Studies of Effects of ELF on Migrating Birds.

47 CFR 400.4 - Application requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... proposed to be funded for the implementation and operation of Phase II E-911 services or migration to an IP... telecommunications services in the implementation and delivery of Phase II E-911 services or for migration to an IP...-911 services or for migration to an IP-enabled emergency network. (2) Project budget. A project budget...

47 CFR 400.4 - Application requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... proposed to be funded for the implementation and operation of Phase II E-911 services or migration to an IP... telecommunications services in the implementation and delivery of Phase II E-911 services or for migration to an IP...-911 services or for migration to an IP-enabled emergency network. (2) Project budget. A project budget...

Navigational Strategies of Migrating Monarch Butterflies

DTIC Science & Technology

2014-11-10

AFRL-OSR-VA-TR-2014-0339 NAVIGATIONAL STRATEGIES OF MIGRATING MONARCH BUTTERFLIES Steven Reppert UNIVERSITY OF MASSACHUSETTS Final Report 11/10/2014...Final Progress Statement to (Dr. Patrick Bradshaw) Contract/Grant Title: Navigational Strategies of Migrating Monarch Butterflies Contract...Grant #: FA9550-10-1-0480 Reporting Period: 01-Sept-10 to 31-Aug-14 Overview of accomplishments: Migrating monarch butterflies (Danaus

The Myth of the Phoenix: Progressive Education, Migration and the Shaping of the Welfare State, 1985-2015

ERIC Educational Resources Information Center

Goossens, Cedric; Van Gorp, Angelo

2016-01-01

Since the 1970s, many western welfare states have been subject both to increased migration and to a renewed interest in progressive education. The present article addresses the question of whether these two phenomena are related and how changing notions of the welfare state shape and are shaped by this relationship. To answer the question, we…

Lamellipodia-based migrations of larval epithelial cells are required for normal closure of the adult epidermis of Drosophila

PubMed Central

Bischoff, Marcus

2012-01-01

Cell migrations are an important feature of animal development. They are, furthermore, essential to wound healing and tumour progression. Despite recent progress, it is still mysterious how cell migration is spatially and temporally regulated during morphogenesis and how cell migration is coordinated with other cellular behaviours to shape tissues and organs. The formation of the abdominal epithelium of Drosophila during metamorphosis provides an attractive system to study morphogenesis. Here, the diploid adult histoblasts replace the polyploid larval epithelial cells (LECs). Using in vivo 4D microscopy, I show that, besides apical constriction and apoptosis, the LECs undergo extensive coordinated migrations. The migrations follow a transition from a stationary (epithelial) to a migratory mode. The migratory behaviour is stimulated by autocrine Dpp signalling. Directed apical lamellipodia-like protrusions propel the cells. Initially, planar cell polarity determines the orientation of LEC migration. While LECs are migrating they also constrict apically, and changes in activity of the small GTPase Rho1 can favour one behaviour over the other. This study shows that the LECs play a more active role in morphogenesis than previously thought, with their migrations contributing to abdominal closure. It furthermore provides insights into how the migratory behaviour of cells is regulated during morphogenesis. PMID:22230614

The Coimbra Community Awareness Project (Portugal). The CDCC's Project No. 7: "The Education and Cultural Development of Migrants."

ERIC Educational Resources Information Center

Rey, Micheline

The Coimbra (Portugal) Community Awareness Project is intended to remedy the social problems caused by migration by helping a local community take responsibility for its own affairs, understand the interactions at work, and enable both residents and emigrants to benefit together from migration. The project operates at several levels under the…

Subsidence of a cementless femoral component influenced by body weight and body mass index.

PubMed

Stihsen, Christoph; Radl, Roman; Keshmiri, Armin; Rehak, Peter; Windhager, Reinhard

2012-05-01

This trial was designed to evaluate the impact of physical characteristics such as body mass index, body weight and height on distal stem migration of a cementless femoral component, as the influence of obesity on the outcome of THA is still debated in literature and conflicting results have been found. In this retrospective cohort study, migration patterns for 102 implants were analysed using the Einzel-Bild-Roentgen-Analyse (EBRA-FCA, femoral component analysis). In all cases the Vision 2000 stem was implanted and combined with the Duraloc acetabular component (DePuy, Warsaw, Indiana). The mean follow-up was 93 months. EBRA-FCA evaluations revealed a mean subsidence of 1.38 mm after two years, 2.06 mm after five and 2.24 mm after seven years. Five stems loosened aseptically. Correlation between increased migration over the whole period and aseptic loosening was highly significant (p < 0.001). Surgical technique had a significant influence on migration and stem stability (p = 0.002) but physical patient characteristics such as body weight over 75 kg and height over 165 cm also significantly influenced stem subsidence towards progressive migration (p = 0.001, p < 0.001). However, a high BMI did not trigger progressive stem migration (p = 0.87). Being of the male gender raised the odds for increased migration (p = 0.03). Physical characteristics such as body weight and height showed significant influence on migration patterns of this cementless femoral component. The operating surgeon should be aware that body weight above 75 kg and height over 165 cm may trigger increased stem migration and the surgeon should aim to fit these prostheses as tightly as possible. However this study demonstrates that a high BMI does not trigger progressive stem migration. Further investigations are needed to confirm our findings.

Spatiotemporal associations between Pacific herring spawn and surf scoter spring migration: evaluating a "silver wave" hypothesis

USGS Publications Warehouse

Lok, E.K.; Esler, Daniel N.; Takekawa, John Y.; De La Cruz, S.W.; Boyd, W.S.; Nysewander, D.R.; Evenson, J.R.; Ward, D.H.

2012-01-01

Surf scoters Melanitta perspicillata are sea ducks that aggregate at spawning events of Pacific herring Clupea pallasi and forage on the eggs, which are deposited in abundance during spring at discrete sites. We evaluated whether migrating scoters followed a ‘silver wave’ of resource availability, analogous to the ‘green wave’ of high-quality foraging conditions that herbivorous waterfowl follow during spring migration. We confirmed that herring spawning activity began later in the year at higher latitudes, creating a northward-progressing wave of short-term localized food availability. Using satellite telemetry and aerial surveys, we documented the chronology of scoter spring migration and the use of stopover locations in relation to herring spawn timing and locations. We found that the migration chronology paralleled the northward progression of herring spawning events. Although there was considerable variability in the timing of both scoter migration and the initiation of herring spawning, the processes were related beyond a coincidental northward progression. During migration, 60% of the tracked scoters visited at least 1 spawn site, and those that used spawn sites were located on spawn sites for approximately one-third of their migration locations. Surf scoters showed close spatiotemporal associations with herring spawning events, confirming that the presence of herring spawn was a factor determining habitat use for many individuals. Surf scoters showed close spatiotemporal associations with herring spawning events, confirming that the presence of herring spawn was a factor determining habitat use for many individuals, a conclusion that is consistent with previous studies which used physiologically based metrics to evaluate the importance of herring spawn.

1980 ASEAN programme roundup: a model in the making.

PubMed

1981-01-01

Association of Southeast Asian Nations (ASEAN) experts and heads of national population programs held their 4th meeting in Singapore from November 24-28, 1980. Program heads resolved to take steps to link their national activities in the population field with those of the ASEAN Population Program and carry out studies and a joint programming exercise in 1981. Progress reports on the following Phase 1 projects were given: 1) integration of population and rural development policies and programs in ASEAN countries including Indonesia, Malaysia, Philippines, Singapore, and Thailand; 2) development of an inter-country modular training program for personnel in population and rural development; 3) multi-media support for population programs in the context of rural development in ASEAN countries; 4) utilization of research findings in population and family planning for policy formulation and program management in ASEAN countries; and 5) migration in relation to rural development. Phase 2 projects approved by ASEAN country participants were also discussed: 1) institutional development and exchange of personnel, 2) women in development, 3) developing and strengthening national population information systems and networks in ASEAN countries, 4) population and development dynamics and the man/resource balance, 5) studies on health and family planning in ASEAN countries, 6) population migration movement and development, and 7) development of ASEAN social indicators.

Area 2: Inexpensive Monitoring and Uncertainty Assessment of CO2 Plume Migration using Injection Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Srinivasan, Sanjay

2014-09-30

In-depth understanding of the long-term fate of CO₂ in the subsurface requires study and analysis of the reservoir formation, the overlaying caprock formation, and adjacent faults. Because there is significant uncertainty in predicting the location and extent of geologic heterogeneity that can impact the future migration of CO₂ in the subsurface, there is a need to develop algorithms that can reliably quantify this uncertainty in plume migration. This project is focused on the development of a model selection algorithm that refines an initial suite of subsurface models representing the prior uncertainty to create a posterior set of subsurface models thatmore » reflect injection performance consistent with that observed. Such posterior models can be used to represent uncertainty in the future migration of the CO₂ plume. Because only injection data is required, the method provides a very inexpensive method to map the migration of the plume and the associated uncertainty in migration paths. The model selection method developed as part of this project mainly consists of assessing the connectivity/dynamic characteristics of a large prior ensemble of models, grouping the models on the basis of their expected dynamic response, selecting the subgroup of models that most closely yield dynamic response closest to the observed dynamic data, and finally quantifying the uncertainty in plume migration using the selected subset of models. The main accomplishment of the project is the development of a software module within the SGEMS earth modeling software package that implements the model selection methodology. This software module was subsequently applied to analyze CO₂ plume migration in two field projects – the In Salah CO₂ Injection project in Algeria and CO₂ injection into the Utsira formation in Norway. These applications of the software revealed that the proxies developed in this project for quickly assessing the dynamic characteristics of the reservoir were highly efficient and yielded accurate grouping of reservoir models. The plume migration paths probabilistically assessed by the method were confirmed by field observations and auxiliary data. The report also documents the application of the software to answer practical questions such as the optimum location of monitoring wells to reliably assess the migration of CO₂ plume, the effect of CO₂-rock interactions on plume migration and the ability to detect the plume under those conditions and the effect of a slow, unresolved leak on the predictions of plume migration.« less

Real-time Implementation of the Waveloc Technique for Monitoring Earthquake Swarms

NASA Astrophysics Data System (ADS)

Maggi, A.; Langet, N.; Michelini, A.

2013-12-01

Monitoring regions with high swarm-type seismicity (e.g. volcanoes, certain tectonic regions) is a challenge for the traditional pick-associate-locate type algorithms that form the basis of most seismicity monitoring software. Over the past few years, new approaches that avoid the association phase by direct migration of some characteristic function of the recorded seismograms have started to be implemented, and have shown great promise (see related abstract on the Waveloc method applied to Piton de la Fournaise volcano). Implementing such methods in real-time is an essential step in proving their usefulness and robustness in swarm-monitoring situations. Here we describe the work in progress on adapting the Waveloc migration technique to real-time operation. The resulting software package, RT-Waveloc, is currently in the prototype stage, and we hope to have a version that can be distributed to the scientific community for beta-testing within a year. The development of RT-Waveloc is financed by the EU NERA project.

Rod Migration Into the Spinal Canal After Posterior Instrumented Fusion Causing Late-Onset Neurological Symptoms.

PubMed

Canavese, Federico; Dmitriev, Petru; Deslandes, Jacques; Samba, Antoine; Dimeglio, Alain; Mansour, Mounira; Rousset, Marie; Dubousset, Jean

2017-01-01

Rod migration into the spinal canal after posterior instrumented fusion is a rare complication causing late-onset neurological symptoms. The purpose of the present study is to report a case of a 13-year-old boy with spastic cerebral palsy and related neuromuscular kyphoscoliosis who developed late-onset neurological deterioration secondary to progressive implant migration into the spinal canal over a 5-year period. A decision was made to remove both rods to achieve decompression. Intraoperative findings were consistent with information gained from preoperative imaging. The rods were found to have an intracanal trajectory at T9-T10 for the right rod and T12-L2 for the left rod. The cause of implant migration, with progressive laminar erosion slow enough to generate a solid mass behind, was progressive kyphosis in a skeletally immature patient with neuromuscular compromise. Fixation type, early surgery, and spasticity management contributed significantly to the presenting condition. Mechanical factors and timing of surgery played a decisive role in this particular presentation. Level IV--Case report and review of the literature.

Enhanced cell migration and apoptosis resistance may underlie the association between high SERPINE1 expression and poor outcome in head and neck carcinoma patients

PubMed Central

Téllez-Gabriel, Marta; León, Xavier; Virós, David; López, Montserrat; Gallardo, Alberto; Céspedes, Maria Virtudes; Casanova, Isolda; López-Pousa, Antonio; Mangues, Maria Antonia; Quer, Miquel; Barnadas, Agustí; Mangues, Ramón

2015-01-01

High SERPINE1 expression is a common event in head and neck squamous cell carcinoma (HNSCC); however, whether it plays a role in determining clinical outcome remains still unknown. We studied SERPINE1 as a prognostic marker in two HNSCC patient cohorts. In a retrospective study (n = 80), high expression of SERPINE1 was associated with poor progression-free (p = 0.022) and cancer-specific (p = 0.040) survival. In a prospective study (n = 190), high SERPINE1 expression was associated with poor local recurrence-free (p = 0.022), progression-free (p = 0.002) and cancer-specific (p = 0.006) survival. SERPINE1 expression was identified as an independent risk factor for progression-free survival in patients treated with chemo-radiotherapy or radiotherapy (p = 0.043). In both patient cohorts, high SERPINE1 expression increased the risk of metastasis spread (p = 0.045; p = 0.029). The association between SERPINE1 expression and survival was confirmed using the HNSCC cohort included in The Cancer Genome Atlas project (n = 507). Once again, patients showing high expression had a poorer survival (p < 0.001). SERPINE1 over-expression in HNSCC cells reduced cell proliferation and enhanced migration. It also protected cells from cisplatin-induced apoptosis, which was accompanied by PI3K/AKT pathway activation. Downregulation of SERPINE1 expression had the opposite effect. We propose SERPINE1 expression as a prognostic marker that could be used to stratify HNSCC patients according to their risk of recurrence. PMID:26359694

On the Ideological Conditions of Canadian Independence

ERIC Educational Resources Information Center

Lawson, Robert F.

1975-01-01

Article focused on the effect of international professional migration as that migration raises critical questions of appropriate human resource utilization for economically progressive and politically automnomous development in Canada. (Author/RK)

Population and labour force projections for 27 European countries, 2002-052: impact of international migration on population ageing: Projections de population et de population active pour 27 pays européens 2002-052: impact de la migration internationale sur le vieillissement de la population.

PubMed

Bijak, Jakub; Kupiszewska, Dorota; Kupiszewski, Marek; Saczuk, Katarzyna; Kicinger, Anna

2007-03-01

Population and labour force projections are made for 27 selected European countries for 2002-052, focussing on the impact of international migration on population and labour force dynamics. Starting from single scenarios for fertility, mortality and economic activity, three sets of assumptions are explored regarding migration flows, taking into account probable policy developments in Europe following the enlargement of the EU. In addition to age structures, various support ratio indicators are analysed. The results indicate that plausible immigration cannot offset the negative effects of population and labour force ageing.

Boundary migration and disappearance of voids in Alpha-Al2O3 at 2000 deg C

NASA Technical Reports Server (NTRS)

Komatsu, M.; Fujita, H.

1984-01-01

A series of photographs taken with Osaka University's high temperature 3MV electron microscope of alpha-A1(z)O(3) at 2000 C is presented. The dynamic study shows grain boundary migration in progress and demonstrates that disappearance of voids is controlled by boundary migration.

Tumor cell migration in complex microenvironments

PubMed Central

Polacheck, William J.; Zervantonakis, Ioannis K.; Kamm, Roger D.

2012-01-01

Tumor cell migration is essential for invasion and dissemination from primary solid tumors and for the establishment of lethal secondary metastases at distant organs. In vivo and in vitro models enabled identification of different factors in the tumor microenvironment that regulate tumor progression and metastasis. However, the mechanisms by which tumor cells integrate these chemical and mechanical signals from multiple sources to navigate the complex microenvironment remain poorly understood. In this review, we discuss the factors that influence tumor cell migration with a focus on the migration of transformed carcinoma cells. We provide an overview of the experimental and computational methods that allow the investigation of tumor cell migration, and we highlight the benefits and shortcomings of the various assays. We emphasize that the chemical and mechanical stimulus paradigms are not independent and that crosstalk between them motivates the development of new assays capable of applying multiple, simultaneous stimuli and imaging the cellular migratory response in real-time. These next-generation assays will more closely mimic the in vivo microenvironment to provide new insights into tumor progression, inform techniques to control tumor cell migration, and render cancer more treatable. PMID:22926411

Recent progress of particle migration in viscoelastic fluids.

PubMed

Yuan, Dan; Zhao, Qianbin; Yan, Sheng; Tang, Shi-Yang; Alici, Gursel; Zhang, Jun; Li, Weihua

2018-02-13

Recently, research on particle migration in non-Newtonian viscoelastic fluids has gained considerable attention. In a viscoelastic fluid, three dimensional (3D) particle focusing can be easily realized in simple channels without the need for any external force fields or complex microchannel structures compared with that in a Newtonian fluid. Due to its promising properties for particle precise focusing and manipulation, this field has been developed rapidly, and research on the field has been shifted from fundamentals to applications. This review will elaborate the recent progress of particle migration in viscoelastic fluids, especially on the aspect of applications. The hydrodynamic forces on the micro/nano particles in viscoelastic fluids are discussed. Next, we elaborate the basic particle migration in viscoelasticity-dominant fluids and elasto-inertial fluids in straight channels. After that, a comprehensive review on the applications of viscoelasticity-induced particle migration (particle separation, cell deformability measurement and alignment, particle solution exchange, rheometry-on-a-chip and others) is presented; finally, we thrash out some perspectives on the future directions of particle migration in viscoelastic fluids.

Investigations into the Early History of Naturally Produced Spring Chinook Salmon in the Grand Ronde Basin : Fish Research Project Oregon : Annual Progress Report Project Period September 1, 1996 to August 31, 1997.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johasson, Brian C.; Tranquilli, J. Vincent; Keefe, MaryLouise

1998-10-28

We have documented two general life history strategies utilized by juvenile spring chinook salmon in the Grande Ronde River basin: (1) juveniles migrate downstream out of summer rearing areas in the fall, overwinter in river valley habitats, and begin their seaward migration in the spring, and (2) juveniles remain in summer rearing areas through the winter and begin seaward migration in the spring. In migration year 96-97, the patterns evident from migrant trap data were similar for the three Grande Ronde River populations studied, with 42% of the Lostine River migrants and 76% of the Catherine Creek migrants leaving uppermore » rearing areas in the fall. Contrary to past years, the majority (98%) of upper Grande Ronde River migrants moved out in the fall. Total trap catch for the upper Grande Ronde River was exceedingly low (29 salmon), indicating that patterns seen this year may be equivocal. As in previous years, approximately 99% of chinook salmon juveniles moved past our trap at the lower end of the Grande Ronde River valley in the spring, reiterating that juvenile chinook salmon overwinter within the Grande Ronde valley section of the river. PIT-tagged fish were recaptured at Grande Ronde River traps and mainstem dams. Recapture data showed that fish that overwintered in valley habitats left as smolts and arrived at Lower Granite Dam earlier than fish that overwintered in upstream rearing areas. Fish from Catherine Creek that overwintered in valley habitats were recaptured at the dams at a higher rate than fish that overwintered upstream. In this first year of data for the Lostine River, fish tagged during the fall migration were detected at a similar rate to fish that overwintered upstream. Abundance estimates for migration year 96-97 were 70 for the upper Grande Ronde River, 4,316 for the Catherine Creek, and 4,323 for the Lostine River populations. Although present in most habitats, juvenile spring chinook salmon were found in the greatest abundance in pool habitats, particularly alcove and backwater pools. These results were consistent for both summer and winter surveys.« less

Steady state acetabular cartilage wear after bipolar hemiarthroplasty: a case series of 10 patients with radiostereometric analysis.

PubMed

Tsukanaka, Masako; Støen, Ragnhild Ø; Figved, Wender; Frihagen, Frede; Nordsletten, Lars; Röhrl, Stephan M

2017-03-31

Cartilage wear is a concern after hemiarthroplasty. The precise pattern of the progression of wear has not been evaluated. We previously reported the application of radiostereonetric analysis (RSA) for the measurement of cartilage wear in patients. The purpose of this study was to report the amount and the orientation of the steady state wear of cartilage between 1 and 3 years after bipolar hemiarthroplasty. 22 patients with a bipolar hemiarthroplasty for displaced femoral neck fracture were included. 10 patients completed the mean follow up of 37 months. The cartilage wear was evaluated by calculating the migration of the bipolar head in reference to the markers in the acetabulum using RSA. The mean age of the patients at the final follow-up was 80 (range 67-91) years. The 3-D migration was -0.02 mm (SD 0.30) between 1 and 3 years. The migration in each direction was 0.03 mm (SD 0.49) in medial, 0.03 mm (SD 0.14) in proximal and 0.11 mm (SD 0.29) in posterior directions. 2 patients showed migration of more than 0.2 mm. The large initial migration seen in some patients up to 1 year did not progress further. Total wear after 37 months was 0.43 mm (SD 0.17). Cartilage wear progressed slowly in 2 of 10 patients from 1 to 3 years. No pelvic penetration was seen. We believe that RSA will give a basic knowledge about the development and the progression of cartilage wear after hemiarthroplasty.

Microglial SMAD4 regulated by microRNA-146a promotes migration of microglia which support tumor progression in a glioma environment

PubMed Central

Karthikeyan, Aparna; Gupta, Neelima; Tang, Carol; Mallilankaraman, Karthik; Silambarasan, Maskomani; Shi, Meng; Lu, Lei; Ang, Beng Ti; Ling, Eng-Ang; Dheen, S. Thameem

2018-01-01

Glioma tumors constitute a significant portion of microglial cells, which are known to support tumor progression. The present study demonstrates that transforming growth factor-β (TGFβ) signaling pathway in microglia in a glioma environment is involved in tumor progression and pathogenesis. It has been shown that the TGFβ level is elevated in higher grades of gliomas and its signaling pathway regulates tumor progression through phosphorylation of SMAD2 and SMAD3, which form a complex with SMAD4 to regulate target gene transcription. In an in vitro cell line-based model increased protein levels of pSMAD2/3, total SMAD2/3 and SMAD4 were observed in murine BV2 microglia cultured in glioma conditioned medium (GCM), indicative of the activated TGFβ signaling pathway in microglia associated with glioma environment. Immunofluorescence labeling further revealed the expression of SMAD4 in microglial and non-microglial cells of human glioblastomas tissue in vivo. Functional analysis through shRNA-mediated stable knockdown of SMAD4 in microglia revealed the downregulation of the expression of matrix metalloproteinase 9 (MMP9), which has been shown to be involved in tumor progression and cell migration. Further, knockdown of SMAD4 in microglia decreased the migration of microglial cells towards GCM, indicating that SMAD4 promotes microglial migration in glioma environment. In addition, SMAD4 has been shown to be post-transcriptionally regulated by microRNA-146a, which was downregulated in microglia treated with GCM. Overexpression of miR-146a resulted in decreased expression of SMAD4 together with tumor supportive gene MMP9 in microglia, and subsequently suppressed microglial migration towards GCM, possibly through regulation of SMAD4. On the other hand, the cell viability assay revealed decreased viability of glioma cells when they were treated with conditioned medium derived from SMAD4 knockdown microglia or miR-146a overexpressed microglia as compared to glioma cells treated with the medium from control microglial cells. Taken together, the present study suggests that microglial SMAD4 which is epigenetically regulated by miR-146a promotes microglial migration in gliomas and glioma cell viability.

Testing an Emerging Paradigm in Migration Ecology Shows Surprising Differences in Efficiency between Flight Modes

PubMed Central

Duerr, Adam E.; Miller, Tricia A.; Lanzone, Michael; Brandes, Dave; Cooper, Jeff; O'Malley, Kieran; Maisonneuve, Charles; Tremblay, Junior; Katzner, Todd

2012-01-01

To maximize fitness, flying animals should maximize flight speed while minimizing energetic expenditure. Soaring speeds of large-bodied birds are determined by flight routes and tradeoffs between minimizing time and energetic costs. Large raptors migrating in eastern North America predominantly glide between thermals that provide lift or soar along slopes or ridgelines using orographic lift (slope soaring). It is usually assumed that slope soaring is faster than thermal gliding because forward progress is constant compared to interrupted progress when birds pause to regain altitude in thermals. We tested this slope-soaring hypothesis using high-frequency GPS-GSM telemetry devices to track golden eagles during northbound migration. In contrast to expectations, flight speed was slower when slope soaring and eagles also were diverted from their migratory path, incurring possible energetic costs and reducing speed of progress towards a migratory endpoint. When gliding between thermals, eagles stayed on track and fast gliding speeds compensated for lack of progress during thermal soaring. When thermals were not available, eagles minimized migration time, not energy, by choosing energetically expensive slope soaring instead of waiting for thermals to develop. Sites suited to slope soaring include ridges preferred for wind-energy generation, thus avian risk of collision with wind turbines is associated with evolutionary trade-offs required to maximize fitness of time-minimizing migratory raptors. PMID:22558166

Testing an emerging paradigm in migration ecology shows surprising differences in efficiency between flight modes.

PubMed

Duerr, Adam E; Miller, Tricia A; Lanzone, Michael; Brandes, Dave; Cooper, Jeff; O'Malley, Kieran; Maisonneuve, Charles; Tremblay, Junior; Katzner, Todd

2012-01-01

To maximize fitness, flying animals should maximize flight speed while minimizing energetic expenditure. Soaring speeds of large-bodied birds are determined by flight routes and tradeoffs between minimizing time and energetic costs. Large raptors migrating in eastern North America predominantly glide between thermals that provide lift or soar along slopes or ridgelines using orographic lift (slope soaring). It is usually assumed that slope soaring is faster than thermal gliding because forward progress is constant compared to interrupted progress when birds pause to regain altitude in thermals. We tested this slope-soaring hypothesis using high-frequency GPS-GSM telemetry devices to track golden eagles during northbound migration. In contrast to expectations, flight speed was slower when slope soaring and eagles also were diverted from their migratory path, incurring possible energetic costs and reducing speed of progress towards a migratory endpoint. When gliding between thermals, eagles stayed on track and fast gliding speeds compensated for lack of progress during thermal soaring. When thermals were not available, eagles minimized migration time, not energy, by choosing energetically expensive slope soaring instead of waiting for thermals to develop. Sites suited to slope soaring include ridges preferred for wind-energy generation, thus avian risk of collision with wind turbines is associated with evolutionary trade-offs required to maximize fitness of time-minimizing migratory raptors.

DOE Office of Scientific and Technical Information (OSTI.GOV)

T. H. Brikowski; D. L. Norton; D. D. Blackwell

Final project report of natural state modeling effort for The Geysers geothermal field, California. Initial models examined the liquid-dominated state of the system, based on geologic constraints and calibrated to match observed whole rock delta-O18 isotope alteration. These models demonstrated that the early system was of generally low permeability (around 10{sup -12} m{sup 2}), with good hydraulic connectivity at depth (along the intrusive contact) and an intact caprock. Later effort in the project was directed at development of a two-phase, supercritical flow simulation package (EOS1sc) to accompany the Tough2 flow simulator. Geysers models made using this package show that ''simmering'',more » or the transient migration of vapor bubbles through the hydrothermal system, is the dominant transition state as the system progresses to vapor-dominated. Such a system is highly variable in space and time, making the rock record more difficult to interpret, since pressure-temperature indicators likely reflect only local, short duration conditions.« less

Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling.

PubMed

Bjorke, Brielle; Shoja-Taheri, Farnaz; Kim, Minkyung; Robinson, G Eric; Fontelonga, Tatiana; Kim, Kyung-Tai; Song, Mi-Ryoung; Mastick, Grant S

2016-10-22

Oculomotor neurons develop initially like typical motor neurons, projecting axons out of the ventral midbrain to their ipsilateral targets, the extraocular muscles. However, in all vertebrates, after the oculomotor nerve (nIII) has reached the extraocular muscle primordia, the cell bodies that innervate the superior rectus migrate to join the contralateral nucleus. This motor neuron migration represents a unique strategy to form a contralateral motor projection. Whether migration is guided by diffusible cues remains unknown. We examined the role of Slit chemorepellent signals in contralateral oculomotor migration by analyzing mutant mouse embryos. We found that the ventral midbrain expresses high levels of both Slit1 and 2, and that oculomotor neurons express the repellent Slit receptors Robo1 and Robo2. Therefore, Slit signals are in a position to influence the migration of oculomotor neurons. In Slit 1/2 or Robo1/2 double mutant embryos, motor neuron cell bodies migrated into the ventral midbrain on E10.5, three days prior to normal migration. These early migrating neurons had leading projections into and across the floor plate. In contrast to the double mutants, embryos which were mutant for single Slit or Robo genes did not have premature migration or outgrowth on E10.5, demonstrating a cooperative requirement of Slit1 and 2, as well as Robo1 and 2. To test how Slit/Robo midline repulsion is modulated, we found that the normal migration did not require the receptors Robo3 and CXCR4, or the chemoattractant, Netrin 1. The signal to initiate contralateral migration is likely autonomous to the midbrain because oculomotor neurons migrate in embryos that lack either nerve outgrowth or extraocular muscles, or in cultured midbrains that lacked peripheral tissue. Overall, our results demonstrate that a migratory subset of motor neurons respond to floor plate-derived Slit repulsion to properly control the timing of contralateral migration.

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

PubMed Central

Cafferty, Patrick; Xie, Xiaojun; Browne, Kristen; Auld, Vanessa J.

2009-01-01

Glial cells of both vertebrate and invertebrate organisms must migrate to final target regions in order to ensheath and support associated neurons. While recent progress has been made to describe the live migration of glial cells in the developing pupal wing (1), studies of Drosophila glial cell migration have typically involved the examination of fixed tissue. Live microscopic analysis of motile cells offers the ability to examine cellular behavior throughout the migratory process, including determining the rate of and changes in direction of growth. Paired with use of genetic tools, live imaging can be used to determine more precise roles for specific genes in the process of development. Previous work by Silies et al. (2) has described the migration of glia originating from the optic stalk, a structure that connects the developing eye and brain, into the eye imaginal disc in fixed tissue. Here we outline a protocol for examining the live migration of glial cells into the Drosophila eye imaginal disc. We take advantage of a Drosophila line that expresses GFP in developing glia to follow glial cell progression in wild type and in mutant animals. PMID:19590493

Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells

PubMed Central

Dai, Jin; Van Wie, Peter G.; Fai, Leonard Yenwong; Kim, Donghern; Wang, Lei; Poyil, Pratheeshkumar; Luo, Jia; Zhang, Zhuo

2018-01-01

Apigenin is a natural flavonoid which possesses multiple anti-cancer properties such as anti-proliferation, anti-inflammation, and anti-metastasis in many types of cancers including colorectal cancer. Neural precursor cell expressed developmentally downregulated 9 (NEDD9) is a multi-domain scaffolding protein of the Cas family which has been shown to correlate with cancer metastasis and progression. The present study investigates the role of NEDD9 in apigenin-inhibited cell migration, invasion, and metastasis of colorectal adenocarcinoma DLD1 and SW480 cells. The results show that knockdown of NEDD9 inhibited cell migration, invasion, and metastasis and that overexpression of NEDD9 promoted cell migration and invasion of DLD1 cells and SW4890 cells. Apigenin treatment attenuated NEDD9 expression at protein level, resulting in reduced phosphorylations of FAK, Src, and Akt, leading to inhibition on cell migration, invasion, and metastasis of both DLD1 and SW480 cells. The present study has demonstrated that apigenin inhibits cell migration, invasion, and metastasis through NEDD9/Src/Akt cascade in colorectal cancer cells. NEDD9 may function as a biomarker for evaluation of cancer aggressiveness and for selection of therapeutic drugs against cancer progression. PMID:27664007

Documenting trans-Himalayan migration through satellite telemetry: A report on capture, deployment, and tracking of bar-headed goose (Anser indicus)

USGS Publications Warehouse

Javed, Sàlim; Takekawa, John Y.; Douglas, David C.; Rahmani, Asad R.; Choudhury, Binod C.; Landfried, Steven L.; Sharma, Shruti

2000-01-01

Animal movement and migration studies have made significant progress with the use of telemetry. Conventional radio telemetry has been used in numerous studies in different regions. However, the use of this technology is restricted to species with limited range of movement. Applying this tool for long distance migrants is usually unsatisfactory. Other challenges such as hilly terrain or dense vegetation, where getting signals and following animals often become major constraints. These problems and the need to track long distance migrants, particularly birds, led to the development of other technologies with greater spatial coverage, accuracy and ease of tracking. Satellite telemetry technology has overcome many of these problems and has become a very useful tool. There is a greater recognition of the use and benefits of this technology among biologists, managers, and various conservation organizations.Satellite tracking technology has been used extensively in the Western Hemisphere. However until recently, in the Indian sub-continent the use of this technology was limited to one study in 1994 when three Eurasian cranes (Grus grus) were fitted with Platform Terminal Transmitter (PTTs) in Keoladco national Park, Bharatpur and tracked to their Siberian breeding grounds (Higuchi et al., 1994). It took almost six more years for the next international collaborative project to emerge within India. This project, started in winter 1998-99, was the first long-term project using satellite tracking in India (Higuchi et al., 1999). Other than these two studies, no effort has been made previously to demonstrate the use of this technology and its application in the Indian subcontinent.

MicroRNA-199 suppresses cell proliferation, migration and invasion by downregulating RGS17 in hepatocellular carcinoma.

PubMed

Zhang, Wei; Qian, Sheng; Yang, Guowei; Zhu, Liang; Zhou, Bo; Wang, Jianhua; Liu, Rong; Yan, Zhiping; Qu, Xudong

2018-06-15

Hepatocellular carcinoma (HCC), the most common primary tumor of the liver, has a poor prognosis and shows rapid progression. MicroRNAs (miRNAs) play important roles in carcinogenesis and tumor progression. Regulators of G-protein signaling (RGS) are critical for defining G-protein-dependent signal fidelity. RGS17 plays an important role in the regulation of cancer cell proliferation, migration and invasion. Here, we showed that miR-199 was downregulated in a hepatocarcinoma cell line. Overexpression of miR-199 significantly suppressed HCC cell proliferation, migration, and invasion in vitro. RGS17 overexpression promoted HCC cell proliferation, migration, and invasion, and reversed the miR-199 mediated inhibition of proliferation, migration, and invasion. Dual-fluorescence reporter experiments confirmed that miR-199 downregulated RGS17 by direct interaction with the 3'-UTR of RGS17 mRNA. In vivo studies showed that miR-199 overexpression significantly inhibited the growth of tumors. Taken together, the results suggested that miR-199 inhibited tumor growth and metastasis by targeting RGS17. Published by Elsevier B.V.

Comparative Study on Migration, Urbanization and Development in the ESCAP Region (RAS/P13/79). Rev. version.

PubMed

1982-01-01

Development of a study project by the UN Economic and Social Commission for Asia and the Pacific (ESCAP) on migration, urbanization, and development in the following countries is described: Indonesia, Malaysia, Pakistan, Philippines, Republic of Korea, Sri Lanka, and Thailand. The project's immediate goal is to assist decision makers in formulating population redistribution policies. It was recommended that ESCAP develop and test a migration questionnaire to assist member countries in undertaking surveys to study the interrelationships of migration and development. Upon completion of survey manuals to assist in the survey implementation, it was suggested that ESCAP run a series of in-country workshops to discuss the applications of survey results for policy formulation. A national migration survey will be taken in each country in the early 1980s in order to discern pattern and type of population mobility, factors that cause people to move or not to move, and the consequences of migration on places of origin and destination. A sample of 14,000 households in each country will be selected and 1 person of age 15-64 will be chosen as the respondent for each household. the following are some items which will be studied: 1) volume of migration streams within and between metropolitan areas and urban-rural areas; 2) decision making factors; 3) interactions between population movement and family structure, chages in fertility levels, employment, and education; 4) impact of agricultural systems on seasonal movements; 5) contributions of migrants to the cities; and 6) implications of international migration to and from the country. Leading family planning agencies will use these results to develop policy relating to population distribution, industry location, migration laws, regional economic planning, modern technology, and rural education. The management framework of the project is presented. After these results are published, government agencies can utilize them by incorporating direct questions on population movement into the national census, conducting demonstration projects to assess the impact of population movement programs, and training personnel.

Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex

PubMed Central

Martinez-Garay, Isabel; Gil-Sanz, Cristina; Franco, Santos J.; Espinosa, Ana; Molnár, Zoltán

2016-01-01

Cadherins are crucial for the radial migration of excitatory projection neurons into the developing neocortical wall. However, the specific cadherins and the signaling pathways that regulate radial migration are not well understood. Here, we show that cadherin 2 (CDH2) and CDH4 cooperate to regulate radial migration in mouse brain via the protein tyrosine phosphatase 1B (PTP1B) and α- and β-catenins. Surprisingly, perturbation of cadherin-mediated signaling does not affect the formation and extension of leading processes of migrating neocortical neurons. Instead, movement of the cell body and nucleus (nucleokinesis) is disrupted. This defect is partially rescued by overexpression of LIS1, a microtubule-associated protein that has previously been shown to regulate nucleokinesis. Taken together, our findings indicate that cadherin-mediated signaling to the cytoskeleton is crucial for nucleokinesis of neocortical projection neurons during their radial migration. PMID:27151949

STRUCTURAL ECONOMIC CHANGE AND INTERNATIONAL MIGRATION FROM MEXICO AND POLAND

PubMed Central

Massey, Douglas S.; Kalter, Frank; Pren, Karen A.

2010-01-01

In this article we use uniquely comparable data sets from two very different settings to examine how exogenous economic transformations affect the likelihood and selectivity of international out-migration. Specifically, we use data from the Mexican Migration Project to construct event history files predicting first U.S. trips from seven communities in the state of Veracruz, which until recently sent very few migrants abroad. Similarly, using data from the Polish Migration Project, we derive comparable event history files predicting first trips to Germany from four Polish communities, which also sent few migrants abroad before the 1980s. Our analyses suggest that the onset of structural adjustment in both places had a significant effect in raising the probability of international migration, even when controlling for a set of standard variables specified by other theories to influence migration propensity, such as the size of the binational income gap and various indicators of human and social capital. PMID:21765550

Gastric cancer-derived MSC-secreted PDGF-DD promotes gastric cancer progression.

PubMed

Huang, Feng; Wang, Mei; Yang, Tingting; Cai, Jie; Zhang, Qiang; Sun, Zixuan; Wu, Xiaodan; Zhang, Xu; Zhu, Wei; Qian, Hui; Xu, Wenrong

2014-11-01

This study was designed to investigate the role of PDGF-DD secreted by gastric cancer-derived mesenchymal stem cells (GC-MSCs) in human gastric cancer progression. Gastric cancer cells were indirectly co-cultured with GC-MSCs in a transwell system. The growth and migration of gastric cancer cells were evaluated by cell colony formation assay and transwell migration assay, respectively. The production of PDGF-DD in GC-MSCs was determined by using Luminex and ELISA. Neutralization of PDGFR-β by su16f and siRNA interference of PDGF-DD in GC-MSCs was used to demonstrate the role of PDGF-DD produced by GC-MSCs in gastric cancer progression. GC-MSC conditioned medium promoted gastric cancer cell proliferation and migration in vitro and in vivo. Co-culture with GC-MSCs increased the phosphorylation of PDGFR-β in SGC-7901 cells. Neutralization of PDGFR-β by su16f blocked the promoting role of GC-MSC conditioned medium in gastric cancer cell proliferation and migration. Recombinant PDGF-DD duplicated the effects of GC-MSC conditioned medium on gastric cancer cells. Knockdown of PDGF-DD in GC-MSCs abolished its effects on gastric cancer cells in vitro and in vivo. PDGF-DD secreted by GC-MSCs is capable of promoting gastric cancer cell progression in vitro and in vivo. Targeting the PDGF-DD/PDGFR-β interaction between MSCs and gastric cancer cells may represent a novel strategy for gastric cancer therapy.

Theories of transporting processes of Cu in Jiaozhou Bay

NASA Astrophysics Data System (ADS)

Yang, Dongfang; Su, Chunhua; Zhu, Sixi; Wu, Yunjie; Zhou, Wei

2018-02-01

Many marine bays have been polluted along with the rapid development of industry and population size, and understanding the transporting progresses of pollutants is essential to pollution control. In order to better understanding the transporting progresses of pollutants in marine, this paper carried on a comprehensive research of the theories of transporting processes of Cu in Jiaozhou Bay. Results showed that the transporting processes of Cu in this bay could be summarized into seven key theories including homogeneous theory, environmental dynamic theory, horizontal loss theory, source to waters transporting theory, sedimentation transporting theory, migration trend theory and vertical transporting theory, respectively. These theories helpful to better understand the migration progress of pollutants in marine bay.

Exogenous hydrogen sulfide exerts proliferation, anti-apoptosis, migration effects and accelerates cell cycle progression in multiple myeloma cells via activating the Akt pathway.

PubMed

Zheng, Dong; Chen, Ziang; Chen, Jingfu; Zhuang, Xiaomin; Feng, Jianqiang; Li, Juan

2016-10-01

Hydrogen sulfide (H2S), regarded as the third gaseous transmitter, mediates and induces various biological effects. The present study investigated the effects of H2S on multiple myeloma cell progression via amplifying the activation of Akt pathway in multiple myeloma cells. The level of H2S produced in multiple myeloma (MM) patients and healthy subjects was measured using enzyme-linked immunosorbent assay (ELISA). MM cells were treated with 500 µmol/l NaHS (a donor of H2S) for 24 h. The expression levels of phosphorylated-Akt (p-Akt), Bcl-2 and caspase-3 were measured by western blot assay. Cell viability was detected by Cell Counting Kit 8 (CCK-8). The cell cycle was analyzed by flow cytometry. Our results show that the concentration of H2S was higher in MM patients and that it increased in parallel with disease progression. Treating MM cells with 500 µmol/l NaHS for 24 h markedly increased the expression level of Bcl-2 and the activation of p-Akt, however, the expression level of caspase-3 was decreased, cell viability was increased, and cell cycle progression was accelerated in MM cells. NaHS also induced migration in MM cells in transwell migration assay. Furthermore, co-treatment of MM cells with 500 µmol/l NaHS and 50 µmol/l LY294002 for 24 h significantly overset these effects. In conclusion, our findings demonstrate that the Akt pathway contributes to NaHS-induced cell proliferation, migration and acceleration of cell cycle progression in MM cells.

Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dai, Jin; Van Wie, Peter G.; Fai, Leonard Yenwong

Apigenin is a natural flavonoid which possesses multiple anti-cancer properties such as anti-proliferation, anti-inflammation, and anti-metastasis in many types of cancers including colorectal cancer. Neural precursor cell expressed developmentally downregulated 9 (NEDD9) is a multi-domain scaffolding protein of the Cas family which has been shown to correlate with cancer metastasis and progression. The present study investigates the role of NEDD9 in apigenin-inhibited cell migration, invasion, and metastasis of colorectal adenocarcinoma DLD1 and SW480 cells. The results show that knockdown of NEDD9 inhibited cell migration, invasion, and metastasis and that overexpression of NEDD9 promoted cell migration and invasion of DLD1 cellsmore » and SW4890 cells. Apigenin treatment attenuated NEDD9 expression at protein level, resulting in reduced phosphorylations of FAK, Src, and Akt, leading to inhibition on cell migration, invasion, and metastasis of both DLD1 and SW480 cells. The present study has demonstrated that apigenin inhibits cell migration, invasion, and metastasis through NEDD9/Src/Akt cascade in colorectal cancer cells. NEDD9 may function as a biomarker for evaluation of cancer aggressiveness and for selection of therapeutic drugs against cancer progression. - Highlights: • Apigenin inhibits migration, invasion, and metastasis of colorectal cancer cells. • Apigenin downregulates NEDD9. • Apigenin decreases phosphorylations of FAK, Src, and Akt. • Apigenin inhibits cell migration, invasion, and metastasis through NEDD9/Src/Akt.« less

Nasal embryonic LHRH factor plays a role in the developmental migration and projection of gonadotropin-releasing hormone 3 neurons in zebrafish.

PubMed

Palevitch, Ori; Abraham, Eytan; Borodovsky, Natalya; Levkowitz, Gil; Zohar, Yonathan; Gothilf, Yoav

2009-01-01

The initiation of puberty and the functioning of the reproductive system depend on proper development of the hypophysiotropic gonadotropin-releasing hormone (GnRH) system. One critical step in this process is the embryonic migration of GnRH neurons from the olfactory area to the hypothalamus. Using a transgenic zebrafish model, Tg(gnrh3:EGFP), in which GnRH3 neurons and axons are fluorescently labeled, we investigated whether zebrafish NELF is essential for the development of GnRH3 neurons. The zebrafish nelf cDNA was cloned and characterized. During embryonic development, nelf is expressed in GnRH3 neurons and in target sites of GnRH3 projections and perikarya, before the initiation of their migration. Nelf knockdown resulted in a disruption of the GnRH3 system which included absence or misguiding of GnRH3 axonal outgrowth and incorrect or arrested migration of GnRH3 perikarya. These results suggest that Nelf is an important factor in the developmental migration and projection of GnRH3 neurons in zebrafish. Copyright (c) 2008 Wiley-Liss, Inc.

Potential Effects of a Scenario Earthquake on the Economy of Southern California: Baseline County-Level Migration Characteristics and Trends 1995-2000 and 2001-2010

USGS Publications Warehouse

Sherrouse, Benson C.; Hester, David J.

2008-01-01

The Multi-Hazards Demonstration Project (MHDP) is a collaboration between the U.S. Geological Survey (USGS) and various partners from the public and private sectors and academia, meant to improve Southern California's resiliency to natural hazards. In support of the MHDP objectives, the ShakeOut Scenario was developed. It describes a magnitude 7.8 earthquake along the southernmost 300 kilometers (200 miles) of the San Andreas Fault, identified by geoscientists as a plausible event that will cause moderate to strong shaking over much of the eight-county (Imperial, Kern, Los Angeles, Orange, Riverside, San Bernardino, San Diego, and Ventura) Southern California region. This report uses historical, estimated, and projected population data from several Federal and State data sources to estimate baseline characteristics and trends of the region's population migration (that is, changes in a person's place of residence over time). The analysis characterizes migration by various demographic, economic, family, and household variables for the period 1995-2000. It also uses existing estimates (beginning in 2001) of the three components of population change - births, deaths, and migration - to extrapolate near-term projections of county-level migration trends through 2010. The 2010 date was chosen to provide baseline projections corresponding to a two-year recovery period following the November 2008 date that was selected for the occurrence of the ShakeOut Scenario earthquake. The baseline characteristics and projections shall assist with evaluating the effects of inflow and outflow migration trends for alternative futures in which the simulated M7.8 earthquake either does or does not occur and the impact of the event on housing and jobs, as well as community composition and regional economy changes based on dispersion of intellectual, physical, economic, and cultural capital.

DEVELOPING AND IMPLEMENTING A BIRD MIGRATION MONITORING, ASSESSMENT AND PUBLIC OUTREACH PROGRAM FOR YOUR COMMUNITY - THE BIRDCAST PROJECT

EPA Science Inventory

The USEPA has developed a technology transfer handbook for the EMPACt BirdCast bird migration monitoring project. The document is essentially a "How-To" Handbook that addresses the planning and implementation steps that were needed to develop, operate and maintain a program simil...

MiR-21 promoted proliferation and migration in hepatocellular carcinoma through negative regulation of Navigator-3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Zhipeng, E-mail: dr_zpwang@163.com; Yang, Huan; Ren, Lei

2015-09-04

MicroRNA-21 (miR-21) has been well-established and found to be over-expressed in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanism of miR-21 involvement in the development and progression of HCC remains to be understood. In the present study, we firstly identified that the Navigator-3 (NAV-3) gene as a novel direct target of miR-21. Knock-down of NAV-3 using shRNA can rescue the effects of anti-miR-21 inhibitor in HCC cell lines, whereas re-expression of miR-21 using transfection with miR-21 mimics phenocopied the NAV-3 knock-down model. Additionally, miR-21 levels inversely correlated with NAV-3 both in HCCmore » cells and tissues. Knock-down of NAV-3 promoted both the proliferation and migration in HCC cells. Together, our findings suggest an important role for miR-21 in the progression of HCC, which negatively regulated Navigator-3 in the migration of HCC. - Highlights: • Navigator-3 (NAV-3) suppresses proliferation, migration and tumorigenesis of HCC cells. • NAV-3 was a novel target of miR-21. • MiR-21 negatively regulates NAV-3 in HCC.« less

Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells.

PubMed

Dai, Jin; Van Wie, Peter G; Fai, Leonard Yenwong; Kim, Donghern; Wang, Lei; Poyil, Pratheeshkumar; Luo, Jia; Zhang, Zhuo

2016-11-15

Apigenin is a natural flavonoid which possesses multiple anti-cancer properties such as anti-proliferation, anti-inflammation, and anti-metastasis in many types of cancers including colorectal cancer. Neural precursor cell expressed developmentally downregulated 9 (NEDD9) is a multi-domain scaffolding protein of the Cas family which has been shown to correlate with cancer metastasis and progression. The present study investigates the role of NEDD9 in apigenin-inhibited cell migration, invasion, and metastasis of colorectal adenocarcinoma DLD1 and SW480 cells. The results show that knockdown of NEDD9 inhibited cell migration, invasion, and metastasis and that overexpression of NEDD9 promoted cell migration and invasion of DLD1 cells and SW4890 cells. Apigenin treatment attenuated NEDD9 expression at protein level, resulting in reduced phosphorylations of FAK, Src, and Akt, leading to inhibition on cell migration, invasion, and metastasis of both DLD1 and SW480 cells. The present study has demonstrated that apigenin inhibits cell migration, invasion, and metastasis through NEDD9/Src/Akt cascade in colorectal cancer cells. NEDD9 may function as a biomarker for evaluation of cancer aggressiveness and for selection of therapeutic drugs against cancer progression. Copyright © 2016 Elsevier Inc. All rights reserved.

Navigator-3, a modulator of cell migration, may act as a suppressor of breast cancer progression

PubMed Central

Cohen-Dvashi, Hadas; Ben-Chetrit, Nir; Russell, Roslin; Carvalho, Silvia; Lauriola, Mattia; Nisani, Sophia; Mancini, Maicol; Nataraj, Nishanth; Kedmi, Merav; Roth, Lee; Köstler, Wolfgang; Zeisel, Amit; Yitzhaky, Assif; Zylberg, Jacques; Tarcic, Gabi; Eilam, Raya; Wigelman, Yoav; Will, Rainer; Lavi, Sara; Porat, Ziv; Wiemann, Stefan; Ricardo, Sara; Schmitt, Fernando; Caldas, Carlos; Yarden, Yosef

2015-01-01

Dissemination of primary tumor cells depends on migratory and invasive attributes. Here, we identify Navigator-3 (NAV3), a gene frequently mutated or deleted in human tumors, as a regulator of epithelial migration and invasion. Following induction by growth factors, NAV3 localizes to the plus ends of microtubules and enhances their polarized growth. Accordingly, NAV3 depletion trimmed microtubule growth, prolonged growth factor signaling, prevented apoptosis and enhanced random cell migration. Mathematical modeling suggested that NAV3-depleted cells acquire an advantage in terms of the way they explore their environment. In animal models, silencing NAV3 increased metastasis, whereas ectopic expression of the wild-type form, unlike expression of two, relatively unstable oncogenic mutants from human tumors, inhibited metastasis. Congruently, analyses of > 2,500 breast and lung cancer patients associated low NAV3 with shorter survival. We propose that NAV3 inhibits breast cancer progression by regulating microtubule dynamics, biasing directionally persistent rather than random migration, and inhibiting locomotion of initiated cells. PMID:25678558

Platelets Inhibit Migration of Canine Osteosarcoma Cells.

PubMed

Bulla, S C; Badial, P R; Silva, R C; Lunsford, K; Bulla, C

2017-01-01

The interaction between platelets and tumour cells is important for tumour growth and metastasis. Thrombocytopenia or antiplatelet treatment negatively impact on cancer metastasis, demonstrating potentially important roles for platelets in tumour progression. To our knowledge, there is no information regarding the role of platelets in cancer progression in dogs. This study was designed to test whether canine platelets affected the migratory behaviour of three canine osteosarcoma cell lines and to give insights of molecular mechanisms. Intact platelets, platelet lysate and platelet releasate inhibited the migration of canine osteosarcoma cell lines. Addition of blood leucocytes to the platelet samples did not alter the inhibitory effect on migration. Platelet treatment also significantly downregulated the transcriptional levels of SNAI2 and TWIST1 genes. The interaction between canine platelets or molecules released during platelet activation and these tumour cell lines inhibits their migration, which suggests that canine platelets might antagonize metastasis of canine osteosarcoma. This effect is probably due to, at least in part, downregulation of genes related to epithelial-mesenchymal transition. Copyright © 2016. Published by Elsevier Ltd.

Programme on the recyclability of food-packaging materials with respect to food safety considerations: polyethylene terephthalate (PET), paper and board, and plastics covered by functional barriers.

PubMed

Franz, R

2002-01-01

Stimulated by new ecology-driven European and national regulations, news routes of recycling waste appear on the market. Since food packages represent a large percentage of the plastics consumption and since they have a short lifetime, an important approach consists in making new packages from post-consumer used packages. On the other hand, food-packaging regulations in Europe require that packaging materials must be safe. Therefore, potential mass transfer (migration) of harmful recycling-related substances to the food must be excluded and test methods to ensure the safety-in-use of recycled materials for food packaging are needled. As a consequence of this situation, a European research project FAIR-CT98-4318, with the acronym 'Recyclability', was initiated. The project consists of three sections each focusing on a different class of recycled materials: polyethylene terephthalate (PET), paper and board, and plastics covered by functional barriers. The project consortium consists of 28 project members from 11 EU countries. In addition, the project is during its lifetime in discussion with the US Food and Drug Administrations (FDA) to consider also US FDA regulatory viewpoints and to aim, as a consequence, to harmonizable conclusions and recommendations. The paper introduces the project and presents an overview of the project work progress.

Short-term international migration trends in England and Wales from 2004 to 2009.

PubMed

Whitworth, Simon; Loukas, Konstantinos; McGregor, Ian

2011-01-01

Short-term migration estimates for England and Wales are the latest addition to the Office for National Statistics (ONS) migration statistics. This article discusses definitions of short-term migration and the methodology that is used to produce the estimates. Some of the estimates and the changes in the estimates over time are then discussed. The article includes previously unpublished short-term migration statistics and therefore helps to give a more complete picture of the size and characteristics of short-term international migration for England and Wales than has previously been possible. ONS have identified a clear user requirement for short-term migration estimates at local authority (LA) level. Consequently, attention is also paid to the progress that has been made and future work that is planned to distribute England and Wales short-term migration estimates to LA level.

Modeling Quasi-Static and Fatigue-Driven Delamination Migration

NASA Technical Reports Server (NTRS)

De Carvalho, N. V.; Ratcliffe, J. G.; Chen, B. Y.; Pinho, S. T.; Baiz, P. M.; Tay, T. E.

2014-01-01

An approach was proposed and assessed for the high-fidelity modeling of progressive damage and failure in composite materials. It combines the Floating Node Method (FNM) and the Virtual Crack Closure Technique (VCCT) to represent multiple interacting failure mechanisms in a mesh-independent fashion. Delamination, matrix cracking, and migration were captured failure and migration criteria based on fracture mechanics. Quasi-static and fatigue loading were modeled within the same overall framework. The methodology proposed was illustrated by simulating the delamination migration test, showing good agreement with the available experimental data.

Floating Node Method and Virtual Crack Closure Technique for Modeling Matrix Cracking-Delamination Migration

NASA Technical Reports Server (NTRS)

DeCarvalho, Nelson V.; Chen, B. Y.; Pinho, Silvestre T.; Baiz, P. M.; Ratcliffe, James G.; Tay, T. E.

2013-01-01

A novel approach is proposed for high-fidelity modeling of progressive damage and failure in composite materials that combines the Floating Node Method (FNM) and the Virtual Crack Closure Technique (VCCT) to represent multiple interacting failure mechanisms in a mesh-independent fashion. In this study, the approach is applied to the modeling of delamination migration in cross-ply tape laminates. Delamination, matrix cracking, and migration are all modeled using fracture mechanics based failure and migration criteria. The methodology proposed shows very good qualitative and quantitative agreement with experiments.

A landscape scale decision support tool for monitoring bird and bat migration across Wisconsin

USGS Publications Warehouse

Suarez, Manuel J.; Heglund, Patricia J.; Kratt, Robert; Kirsch, Eileen

2008-01-01

This project was initiated to begin addressing the question, “Are there patterns in timing, location, and direction among migrating landbirds?” that have been at the forefront of discussion with our Federal, State, and County partners with regard to siting wind energy projects. Our goal was to explore the use of Nexrad weather data to see if examining 5 or more years’ worth of data would provide us with a sense of the general timing, movement patterns and habitat use by migrating landbirds.

[A cutting obstacle to the harmonious development of the country. The migratory phenomenon in Mali].

PubMed

Diarra, S

1991-04-01

Mali has long been affected by migration because of its location between North Africa and sub-Saharan Africa. Around 80% of the population depends on agriculture or herding for its livelihood. During the rainy season, from June-October, most of the population is busy in agricultural work, but during the dry season from November to May seasonal migration in search of wage employment is common. Seasonal migration is also part of the long tradition of commerce with neighboring countries. Economic difficulties of the last several years have encourage migration to urban centers and foreign countries. The drought that has afflicted Mali for over a decade is a further impetus to migration. 2 types of migration can be distinguished in Mali, shortterm movements of nomads and seasonal migration lasting less than 6 months, and definitive movements lasting longer including rural-urban and international migration. 4 further categories of migration include rural-rural movements especially in the south and in areas of industrial development, rural-urban migrations which are 1 of the most important factors in rapid urban growth especially around Bamako,, urban-urban migration essentially reflecting step migration from rural areas to secondary urban centers and finally to large cities, and urban-rural migration which occurs much less frequently. Over 80% of temporary movements take place within Mali. About 80% of those residing temporarily outside Mali are men. Temporary migrants inside Mali are oriented from the north to the region of Sikasso and the District of Bamako in the south and primarily are young men aged 15-29. There has been a progressive decline in the proportion of nomads in the general population from 7% in 1976 to 4% in 1987. There has been an increase in definitive migration from areas less favored ecologically to more desirable areas. A 1985 demographic survey found that 21% of the population aged 15 or over were migrants. 51.4% of migrants were male. 86% cited the drought and search for work as reasons for migrating. In 1987, foreigners accounted for less than 1% of the population of Mali. Most international migrants go to the Ivory Coast, Senegal, Ghana, the Congo, Gabon, Cameroon, or France. The major causes of migration are poverty and lack of employment opportunities, population pressure on arable land, surplus labor at the household level, displacement of population by dams and large construction projects, and miscellaneous socioeconomic and political reasons such as fascination with the city, desire for better health care, and the wish to be liberated from family pressures. Consequences of migration for sending areas include reduced production due to loss of manpower and instability of households due to absence of members. In the areas of destination, problems may include conflicts between migrants and natives or accentuation of desertification due to deforestation and intensive use of lands.

A non-mitotic role for Aurora kinase A as a direct activator of cell migration upon interaction with PLD, FAK and Src

PubMed Central

Mahankali, Madhu; Henkels, Karen M.; Speranza, Francis; Gomez-Cambronero, Julian

2015-01-01

ABSTRACT Timely activation of Aurora kinase A (AURA, also known as AURKA) is vital for centrosome formation and the progression of mitosis. Nonetheless, it is still unclear if and when other cellular functions are activated by AURA. We report here that Src phosphorylates and activates AURA at T288, and AURA also activates focal adhesion kinase (FAK, also known as PTK2), leading to initiation of cell movement. An additional and new way by which AURA is regulated, is by phospholipase D2 (PLD2), which causes AURA activation. In addition, AURA phosphorylates PLD, so both proteins engage in a positive reinforcement loop. AURA and PLD2 form a protein–protein complex and colocalize to cytoplasmic regions in cells. The reason why PLD activates AURA is because of the production of phosphatidic acid by the lipase, which binds directly to AURA, with the region E171–E211 projected to be a phosphatidic-acid-binding pocket. Furthermore, this direct interaction with phosphatidic acid enhances tubulin polymerization and cooperates synergistically with AURA, FAK and Src in yielding a fully effectual cellular migration. Thus, Src and FAK, and PLD and phosphatidic acid are new upstream regulators of AURA that mediate its role in the non-mitotic cellular function of cell migration. PMID:25501815

Migrating Professional Knowledge: Progressions, Regressions, and Dislocations

ERIC Educational Resources Information Center

Slade, Bonnie L.

2015-01-01

Drawing on practice-based learning theory, this chapter examines issues pertaining to the deskilling of immigrant professionals in Canada. It argues that adult educators need to have an awareness of transnational migration dynamics and work in meaningful ways to keep immigrant professionals connected to professional knowledge practices.

Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex.

PubMed

Martinez-Garay, Isabel; Gil-Sanz, Cristina; Franco, Santos J; Espinosa, Ana; Molnár, Zoltán; Mueller, Ulrich

2016-06-15

Cadherins are crucial for the radial migration of excitatory projection neurons into the developing neocortical wall. However, the specific cadherins and the signaling pathways that regulate radial migration are not well understood. Here, we show that cadherin 2 (CDH2) and CDH4 cooperate to regulate radial migration in mouse brain via the protein tyrosine phosphatase 1B (PTP1B) and α- and β-catenins. Surprisingly, perturbation of cadherin-mediated signaling does not affect the formation and extension of leading processes of migrating neocortical neurons. Instead, movement of the cell body and nucleus (nucleokinesis) is disrupted. This defect is partially rescued by overexpression of LIS1, a microtubule-associated protein that has previously been shown to regulate nucleokinesis. Taken together, our findings indicate that cadherin-mediated signaling to the cytoskeleton is crucial for nucleokinesis of neocortical projection neurons during their radial migration. © 2016. Published by The Company of Biologists Ltd.

Trade and migration: the case of NAFTA.

PubMed

Martin, P L

1993-01-01

"This article provides background information on NAFTA [the North American Free Trade Agreement], reviews data on its economic effects, and summarizes studies and projections of NAFTA's likely effects on Mexico-to-U.S. migration. Migration factors (demand-pull, supply-push, and networks) are examined to determine whether NAFTA's effect on economic development particularly in the border areas will accelerate or retard migration. The conclusion is that NAFTA is likely to produce a temporary migration hump, slightly raising already high migration levels in the 1990s, but reducing the volume of Mexico-to-U.S. migration that would otherwise occur over subsequent decades." excerpt

MiR-21 promoted proliferation and migration in hepatocellular carcinoma through negative regulation of Navigator-3.

PubMed

Wang, Zhipeng; Yang, Huan; Ren, Lei

2015-09-04

MicroRNA-21 (miR-21) has been well-established and found to be over-expressed in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanism of miR-21 involvement in the development and progression of HCC remains to be understood. In the present study, we firstly identified that the Navigator-3 (NAV-3) gene as a novel direct target of miR-21. Knock-down of NAV-3 using shRNA can rescue the effects of anti-miR-21 inhibitor in HCC cell lines, whereas re-expression of miR-21 using transfection with miR-21 mimics phenocopied the NAV-3 knock-down model. Additionally, miR-21 levels inversely correlated with NAV-3 both in HCC cells and tissues. Knock-down of NAV-3 promoted both the proliferation and migration in HCC cells. Together, our findings suggest an important role for miR-21 in the progression of HCC, which negatively regulated Navigator-3 in the migration of HCC. Copyright © 2015 Elsevier Inc. All rights reserved.

Suppression of mitochondrial respiration with auraptene inhibits the progression of renal cell carcinoma: involvement of HIF-1α degradation.

PubMed

Jang, Yunseon; Han, Jeongsu; Kim, Soo Jeong; Kim, Jungim; Lee, Min Joung; Jeong, Soyeon; Ryu, Min Jeong; Seo, Kang-Sik; Choi, Song-Yi; Shong, Minho; Lim, Kyu; Heo, Jun Young; Kweon, Gi Ryang

2015-11-10

Renal cell carcinoma (RCC) progression resulting from the uncontrolled migration and enhanced angiogenesis is an obstacle to effective therapeutic intervention. Tumor metabolism has distinctive feature called Warburg effect, which enhances the aerobic glycolysis rapidly supplying the energy for migration of tumor. To manipulate this metabolic change characteristic of aggressive tumors, we utilized the citrus extract, auraptene, known as a mitochondrial inhibitor, testing its anticancer effects against the RCC4 cell line. We found that auraptene impaired RCC4 cell motility through reduction of mitochondrial respiration and glycolytic pathway-related genes. It also strongly disrupted VEGF-induced angiogenesis in vitro and in vivo. Hypoxia-inducible factor 1a (HIF-1a), a key regulator of cancer metabolism, migration and angiogenesis that is stably expressed in RCCs by virtue of a genetic mutation in the von Hippel-Lindau (VHL) tumor-suppressor protein, was impeded by auraptene, which blocked HIF-1a translation initiation without causing cytotoxicity. We suggest that blockade HIF-1a and reforming energy metabolism with auraptene is an effective approach for suspension RCC progression.

Mesenchyme Homeobox 2 Enhances Migration of Endothelial Colony Forming Cells Exposed to Intrauterine Diabetes Mellitus.

PubMed

Gohn, Cassandra R; Blue, Emily K; Sheehan, BreAnn M; Varberg, Kaela M; Haneline, Laura S

2017-07-01

Diabetes mellitus (DM) during pregnancy has long-lasting implications for the fetus, including cardiovascular morbidity. Previously, we showed that endothelial colony forming cells (ECFCs) from DM human pregnancies have decreased vasculogenic potential. Here, we evaluate whether the molecular mechanism responsible for this phenotype involves the transcription factor, Mesenchyme Homeobox 2 (MEOX2). In human umbilical vein endothelial cells, MEOX2 upregulates cyclin-dependent kinase inhibitor expression, resulting in increased senescence and decreased proliferation. We hypothesized that dysregulated MEOX2 expression in neonatal ECFCs from DM pregnancies decreases network formation through increased senescence and altered cell cycle progression. Our studies show that nuclear MEOX2 is increased in ECFCs from DM pregnancies. To determine if MEOX2 is sufficient and/or required to induce impaired network formation, MEOX2 was overexpressed and depleted in ECFCs from control and DM pregnancies, respectively. Surprisingly, MEOX2 overexpression in control ECFCs resulted in increased network formation, altered cell cycle progression, and increased senescence. In contrast, MEOX2 knockdown in ECFCs from DM pregnancies led to decreased network formation, while cell cycle progression and senescence were unaffected. Importantly, migration studies demonstrated that MEOX2 overexpression increased migration, while MEOX2 knockdown decreased migration. Taken together, these data suggest that altered migration may be mediating the impaired vasculogenesis of ECFCs from DM pregnancies. While initially believed to be maladaptive, these data suggest that MEOX2 may serve a protective role, enabling increased vessel formation despite exposure to a DM intrauterine environment. J. Cell. Physiol. 232: 1885-1892, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Preparing for climate change: Forestry and assisted migration

Treesearch

Mary I. Williams; R. Kasten Dumroese

2013-01-01

Although plants have moved across the landscape in response to changing climate for millennia, projections of contemporary climate change suggest that forest tree species and populations will need to migrate faster than their natural ability. Therefore, climate change adaptation strategies, such as assisted migration, have gained attention since 2007. Effective...

Project Integration Architecture: Implementation of the CORBA-Served Application Infrastructure

NASA Technical Reports Server (NTRS)

Jones, William Henry

2005-01-01

The Project Integration Architecture (PIA) has been demonstrated in a single-machine C++ implementation prototype. The architecture is in the process of being migrated to a Common Object Request Broker Architecture (CORBA) implementation. The migration of the Foundation Layer interfaces is fundamentally complete. The implementation of the Application Layer infrastructure for that migration is reported. The Application Layer provides for distributed user identification and authentication, per-user/per-instance access controls, server administration, the formation of mutually-trusting application servers, a server locality protocol, and an ability to search for interface implementations through such trusted server networks.

Progressive migration of broken Kirschner wire into the proximal tibia following tension-band wiring technique of a patellar fracture--case report.

PubMed

Konda, Sanjit R; Dayan, Alan; Egol, Kenneth A

2012-01-01

Wire breakage and migration is a known complication of using a wire tension band construct to treat displaced patella fractures. We report a case of a broken K-wire that migrated from the patella completely into the proximal tibia without complication 9 years after the index surgery. This report highlights the fact that wire migration can occur long after fracture healing and be relatively asymptomatic. But because the complications of wire migration can be deadly, it requires diligence on the part of the physician to educate the patient that new knee pain after operative fixation requires formal evaluation by the treating surgeon.

The movement of people in Asia: internal, intra-regional and international migration.

PubMed

Lim, L L; Abella, M

1994-01-01

"The comprehensive overview of Asian-Pacific migration summarizes early population movements during the colonial period and describes the major types of contemporary Asian population movements: (1) environmental refugees, (2) political refugees, (3) internal population movements, (4) contract labor migration, (5) migration of permanent settlers, (6) business related movements and tourism. Projections of net international migration are given. Population growth, employment absorption and emigration pressures are likely to contribute to a large mobility potential for Asia, with significant implications for Australia." excerpt

Comparing internal migration across the countries of Latin America: A multidimensional approach

PubMed Central

Bernard, Aude; Rowe, Francisco; Bell, Martin; Ueffing, Philipp; Charles-Edwards, Elin

2017-01-01

While considerable progress has been made in understanding the way particular aspects of internal migration, such as its intensity, age profile and spatial impact, vary between countries around the world, little attention to date has been given to establishing how these dimensions of migration interact in different national settings. We use recently developed measures of internal migration that are scale-independent to compare the overall intensity, age composition, spatial impact, and distance profile of internal migration in 19 Latin American countries. Comparisons reveal substantial cross-national variation but cluster analysis suggests the different dimensions of migration evolve systematically to form a broad sequence characterised by low intensities, young ages at migration, unbalanced flows and high friction of distance at lower levels of development, trending to high intensities, an older age profile of migration, more closely balanced flows and lower friction of distance at later stages of development. However, the transition is not linear and local contingencies, such as international migration and political control, often distort the migration-development nexus, leading to unique migration patterns in individual national contexts. PMID:28328932

Analysis of urban-rural population dynamics for China.

PubMed

Shen, J

1991-12-01

The population dynamics of China are presented in a multiregional demographic model using regional estimates or mortality and migration based on the 1% population sample survey in 1987. An open ended population account is generated for period cohort a, gender g of region i (urban) and j (rural) using population, birth, death, and migration. Demographic rates and equations for flows of nonsurviving migrants of period cohort a of gender g are estimated using the forward demographic rate definition. Out-migration rates for period cohort a of gender g are defined by migration flow divided by the initial population. The death rate for period cohort A1 and A are estimated using a single region method. Death and migration rates are simultaneously estimated with an iterative procedure. The population accounts estimates and demographic rates are provided for the period ending 1986-87 for male births, males in period cohorts 10 and 20, female births, and females in period cohorts 10 and 20. The urban and rural population projection model is based on the population accounts concept and assumes fixed rates of mortality, migration, and normal fertility for the base year 1987. The results of this projection are a population of 1090 million that will grow to 1304 million in 2000, 1720 million in 2050, and 1791 million in 2087. Urban population will expand from 44.2% in 1988 to 46.6% in 2000, and 54.7% in 2087. The labor population of males 18-65 years and females 18-60 years will increase from 58.8% in 1988 to 59.7% in 2000 and decline to 58.4% by 2087. The old age population of males 65 years and females 60 years will increase from 6.5% in 1988 to 7.9% in 2000, and 16.3% in 2087. The mean age increased from 28.3 years in 1988 to 37 in 2087. Urban population may be underprojected; migration problems are recognized. Fertility also is likely to decline. An alternative projection (B) is given to account for the U-shape distribution and urban fertility of 1.8 in 2000, increasing to and stabilizing at 2.2 in 2020, such that population estimates for 2000 are 1291 and 1524 for 2087 with a peak in 2048 of 1573. A faster fertility decline is also used to generate projection C. The author's projections A, B, and C, which are based on more recent data and a more realistic model, are than the "objective projection" and than the "warning projection" generated by China's Population Census Office based on 1982 census data.

Migration within China and from China to the USA: the effects of migration networks, selectivity, and the rural political economy in Fujian Province.

PubMed

Liang, Zai; Chunyu, Miao David

2013-07-01

This paper tests a new strategy for simultaneously studying internal migration within, and international migration from, China. Our theoretical discussion draws on ideas from migration-networks theory and studies of the transition to a market-oriented economy. Data collection is modelled on the Mexican Migration Project. We find that education is more important in initiating internal migration than international migration. Second, although the role of migration networks at a community level seems similar to that for Mexico-USA migration, the networks at a family level show a different pattern. Third, there is evidence that internal and international migration are competing options. Finally, we find that individuals with cadres (public officials) in the family are less likely to undertake internal migration, but more likely to participate in international migration, a finding that highlights the continuing significance of the cadres in coastal rural China.

Assessing relationships between cattle egret migration and meteorology in the southwest Pacific: a review

NASA Astrophysics Data System (ADS)

Bridgman, H. A.; Maddock, M.; Geering, D. J.

The evolution of research into meteorological factors affecting the migration of the Cattle Egret (Ardeola ibis coromandus) in the southwestern Pacific region (Australia, New Zealand and the Tasman Sea) - from ground-based studies dependent on volunteer observers to a pilot satellite-tracking project - is reviewed and the results are related to the literature on bird migration. The predominant pattern is a seasonal migration from breeding colonies in southeast Queensland and northern New South Wales which takes place in stages along the east coastal plain under favourable meteorological conditions. Migration outward (southward) occurs in February through April and return to the breeding colonies occurs in October and November. Wintering destinations include Tasmania, southern Victoria and parts of New Zealand. Favourable meteorological conditions for migration southward include:moderate north to northwest airflow behind a high; light and variable winds in a high or col; and light and variable winds over New South Wales with moderate westerlies over Victoria and Tasmania. A satellite-tracking project helped to validate findings from the ground-based studies, provided additional information not otherwise obtainable, and demonstrated the potential of the technique to further clarify the relation between timing and staging of migration, and meteorology.

Potential Roles of GLUT12 for Glucose Sensing and Cellular Migration in MCF-7 Human Breast Cancer Cells Under High Glucose Conditions.

PubMed

Matsui, Chihiro; Takatani-Nakase, Tomoka; Maeda, Sachie; Nakase, Ikuhiko; Takahashi, Koichi

2017-12-01

Recent reports have indicated that hyperglycaemia is associated with breast cancer progression. High glucose conditions corresponding to hyperglycaemia significantly promote migration of MCF-7 human breast cancer cells, however, little is known about the mechanisms of glucose sensing for the acquisition of migratory properties by MCF-7 cells. This study investigated glucose sensing and mediation, which are responsible for the high motility of MCF-7 cells. We evaluated the migration of MCF-7 cells cultured in high glucose-containing medium and essential regulatory factors from the perspective of the glucose transport system. We demonstrated that glucose transporter 12 (GLUT12) protein level increased in MCF-7 cells and co-localized with actin organization under high glucose conditions. Moreover, GLUT12-knockdown completely abrogated high glucose-induced migration, indicating that GLUT12 functionally participates in sensing high glucose concentrations. GLUT12 plays a critical role in the model of breast cancer progression through high glucose concentrations. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Social Capital and International Migration from Latin America

PubMed Central

Massey, Douglas S.; Aysa-Lastra, María

2011-01-01

We combine data from the Latin American Migration Project and the Mexican Migration Project to estimate models predicting the likelihood of taking of first and later trips to the United States from five nations: Mexico, the Dominican Republic, Costa Rica, Nicaragua, and Peru. The models test specific hypotheses about the effects of social capital on international migration and how these effects vary with respect to contextual factors. Our findings confirm the ubiquity of migrant networks and the universality of social capital effects throughout Latin America. They also reveal how the sizes of these effects are not uniform across settings. Social capital operates more powerfully on first as opposed to later trips and interacts with the cost of migration. In addition, effects are somewhat different when considering individual social capital (measuring strong ties) and community social capital (measuring weak ties). On first trips, the effect of strong ties in promoting migration increases with distance whereas the effect of weak ties decreases with distance. On later trips, the direction of effects for both individual and community social capital is negative for long distances but positive for short distances. PMID:21915379

MicroRNA-187 regulates gastric cancer progression by targeting the tumor suppressor CRMP1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ren, Lian; Li, Fang; Di, Maojun

Aberrant expression of microRNAs contributes to the initiation and progression of numerous human cancers. The underlying effects and molecular mechanisms of microRNA-187 (miR-187) in gastric cancer (GC) remain unclear. The present study reports that miR-187 was significantly overexpressed in GC tissues compared to that in non-tumor tissues and was associated with malignant clinical factors such as depth of invasion (P = 0.005), tumor size (P = 0.024), lymph node metastasis (P = 0.048), and TNM stage (P = 0.035). Additionally, miR-187 promoted tumor growth in vivo, and significantly increased migration, invasion, and proliferation, but inhibited apoptosis in GC cells. It was found that collapsin response mediator protein 1 (CRMP1),more » a tumor suppressor, was a direct downstream target of miR-187 in GC. Furthermore, CRMP1 silencing resulted in similar effects on cell proliferation, migration, and apoptosis as those of miR-187 overexpressing GC cells. Additionally, the effects of miR-187 inhibitor on cell migration and cell apoptosis were reversed by CRMP1 downregulation. In summary, miR-187 promotes tumor progression by regulating CRMP1 expression in GC and may thus be a potential prognostic marker and a therapeutic target in GC. - Highlights: • miR-187 was significantly overexpressed in GC tissues and associated with malignant clinical factors. • miR-187 significantly increased migration, invasion, and proliferation, but inhibited apoptosis in GC cells. • CRMP1 tumor suppressor is a direct target of miR-187 in GC. • Overexpression of miR-187 promoted GC progression by targeting tumor suppressor gene CRMP1.« less

Determinants of spring migration departure decision in a bat.

PubMed

Dechmann, Dina K N; Wikelski, M; Ellis-Soto, D; Safi, K; O'Mara, M Teague

2017-09-01

Migratory decisions in birds are closely tied to environmental cues and fat stores, but it remains unknown if the same variables trigger bat migration. To learn more about the rare phenomenon of bat migration, we studied departure decisions of female common noctules ( Nyctalus noctula ) in southern Germany. We did not find the fattening period that modulates departure decisions in birds. Female noctules departed after a regular evening foraging session, uniformly heading northeast. As the day of year increased, migratory decisions were based on the interactions among wind speed, wind direction and air pressure. As the migration season progressed, bats were likely to migrate on nights with higher air pressure and faster tail winds in the direction of travel, and also show high probability of migration on low-pressure nights with slow head winds. Common noctules thus monitor complex environmental conditions to find the optimal migration night. © 2017 The Authors.

Climate change as a driver for future human migration

NASA Astrophysics Data System (ADS)

Chen, M.; Ricke, K.; Caldeira, K.

2016-12-01

Human migration is driven by a multitude of factors, both socioeconomic and environmental. However, as impacts of anthropogenic climate change emerge and grow, it is widely conjectured that climate change will induce migration of human populations from areas that are adversely affected by climate change to areas that are less adversely or positively affected by climate change. Both low- and high-frequency climate changes have been empirically linked to migration in areas across the globe, but there has been little global-scale quantitative analysis projecting the scale and geography of climate-motivated migration. Considering temperature and precipitation in isolation from all other factors, here we project climate-driven impacts on the areal-density of human population. From this, we infer potential destinations and origins for the climate-motivated migration. Our results indicate that tropical and sub-tropical countries are the largest likely sources of migrants, with India being the country with the greatest number of potential climate emigrants. Global warming has the potential to motivate hundreds of millions of people to migrate in the coming decades, largely from warm tropical and subtropical countries to cooler temperate countries. Migration decisions will depend on many factors beyond climate; nevertheless our work establishes a foundation for quantifying future climate-motivated migration that can act as a starting point of more comprehensive assessments. The large number of potential climate migrants indicated by our analyses provides additional incentive to reduce greenhouse gas emissions, take adaptive measures, and carefully consider migration policy.

The MUC4 membrane-bound mucin regulates esophageal cancer cell proliferation and migration properties: Implication for S100A4 protein

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bruyere, Emilie; Jonckheere, Nicolas; Frenois, Frederic

2011-09-23

Highlights: {yields} Loss of MUC4 reduces proliferation of esophageal cancer cells. {yields} MUC4 inhibition impairs migration of esophageal cancer cells but not their invasion. {yields} Loss of MUC4 significantly reduces in vivo tumor growth. {yields} Decrease of S100A4 induced by MUC4 inhibition impairs proliferation and migration. -- Abstract: MUC4 is a membrane-bound mucin known to participate in tumor progression. It has been shown that MUC4 pattern of expression is modified during esophageal carcinogenesis, with a progressive increase from metaplastic lesions to adenocarcinoma. The principal cause of development of esophageal adenocarcinoma is the gastro-esophageal reflux, and MUC4 was previously shown tomore » be upregulated by several bile acids present in reflux. In this report, our aim was thus to determine whether MUC4 plays a role in biological properties of human esophageal cancer cells. For that stable MUC4-deficient cancer cell lines (shMUC4 cells) were established using a shRNA approach. In vitro (proliferation, migration and invasion) and in vivo (tumor growth following subcutaneous xenografts in SCID mice) biological properties of shMUC4 cells were analyzed. Our results show that shMUC4 cells were less proliferative, had decreased migration properties and did not express S100A4 protein when compared with MUC4 expressing cells. Absence of MUC4 did not impair shMUC4 invasiveness. Subcutaneous xenografts showed a significant decrease in tumor size when cells did not express MUC4. Altogether, these data indicate that MUC4 plays a key role in proliferative and migrating properties of esophageal cancer cells as well as is a tumor growth promoter. MUC4 mucin appears thus as a good therapeutic target to slow-down esophageal tumor progression.« less

TGF-β-Induced Transcription Sustains Amoeboid Melanoma Migration and Dissemination

PubMed Central

Cantelli, Gaia; Orgaz, Jose L.; Rodriguez-Hernandez, Irene; Karagiannis, Panagiotis; Maiques, Oscar; Matias-Guiu, Xavier; Nestle, Frank O.; Marti, Rosa M.; Karagiannis, Sophia N.; Sanz-Moreno, Victoria

2015-01-01

Summary Cell migration underlies metastatic dissemination of cancer cells, and fast “amoeboid” migration in the invasive fronts of tumors is controlled by high levels of actomyosin contractility. How amoeboid migration is regulated by extracellular signals and sustained over time by transcriptional changes is not fully understood. Transforming growth factor β (TGF-β) is well known to promote epithelial-to-mesenchymal transition (EMT) and contribute to metastasis, but melanocytes are neural crest derivatives that have undergone EMT during embryonic development. Surprisingly, we find that in melanoma, TGF-β promotes amoeboid features such as cell rounding, membrane blebbing, high levels of contractility, and increased invasion. Using genome-wide transcriptomics, we find that amoeboid melanoma cells are enriched in a TGF-β-driven signature. We observe that downstream of TGF-β, SMAD2 and its adaptor CITED1 control amoeboid behavior by regulating the expression of key genes that activate contractile forces. Moreover, CITED1 is highly upregulated during melanoma progression, and its high expression is associated with poor prognosis. CITED1 is coupled to a contractile-rounded, amoeboid phenotype in a panel of 16 melanoma cell lines, in mouse melanoma xenografts, and in 47 human melanoma patients. Its expression is also enriched in the invasive fronts of lesions. Functionally, we show how the TGF-β-SMAD2-CITED1 axis promotes different steps associated with progression: melanoma detachment from keratinocytes, 2D and 3D migration, attachment to endothelial cells, and in vivo lung metastatic initial colonization and outgrowth. We propose a novel mechanism by which TGF-β-induced transcription sustains actomyosin force in melanoma cells and thereby promotes melanoma progression independently of EMT. PMID:26526369

A pilgrim's progress: Seeking meaning in primordial germ cell migration.

PubMed

Cantú, Andrea V; Laird, Diana J

2017-10-01

Comparative studies of primordial germ cell (PGC) development across organisms in many phyla reveal surprising diversity in the route of migration, timing and underlying molecular mechanisms, suggesting that the process of migration itself is conserved. However, beyond the perfunctory transport of cellular precursors to their later arising home of the gonads, does PGC migration serve a function? Here we propose that the process of migration plays an additional role in quality control, by eliminating PGCs incapable of completing migration as well as through mechanisms that favor PGCs capable of responding appropriately to migration cues. Focusing on PGCs in mice, we explore evidence for a selective capacity of migration, considering the tandem regulation of proliferation and migration, cell-intrinsic and extrinsic control, the potential for tumors derived from failed PGC migrants, the potential mechanisms by which migratory PGCs vary in their cellular behaviors, and corresponding effects on development. We discuss the implications of a selective role of PGC migration for in vitro gametogenesis. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

The Impact of Migrations on the Health Services for Rare Diseases in Europe: The Example of Haemoglobin Disorders

PubMed Central

Angastiniotis, Michalis; Vives Corrons, Joan-Lluis; Soteriades, Elpidoforos S.; Eleftheriou, Androulla

2013-01-01

Migration from different parts of the world to several European countries leads to the introduction of haemoglobinopathy genes into the population, which creates several demanding needs for prevention and treatment services for Hb disorders. In this paper we examined the degree to which European health services have responded to such challenges and in particular to health services necessary to address the needs of patients with thalassaemia and sickle cell disease (SCD). Information on available services was obtained from international organizations, collaborated European project, and the Thalassaemia International Federation (TIF) Databases, which include information from published surveys, registries, field trips, and delegation visits to countries and regions by expert advisors, local associations, and other collaborators' reports. Results show that countries with traditional strong prevention and treatment programs are well prepared to face the above challenges, while others are urgently needed to address these problems in a systematic way. The Thalassaemia International Federation (TIF) is committed to monitor the progress, raise awareness, and support the promotion of more immigrant-oriented health policies to ensure their integration in society and their access to appropriate, adequate, and timely health services. PMID:23576907

Type III TGF-β Receptor Enhances Colon Cancer Cell Migration and Anchorage-Independent Growth12

PubMed Central

Gatza, Catherine E; Holtzhausen, Alisha; Kirkbride, Kellye C; Morton, Allyson; Gatza, Michael L; Datto, Michael B; Blobe, Gerard C

2011-01-01

The type III TGF-β receptor (TβRIII or betagylcan) is a TGF-β superfamily coreceptor with emerging roles in regulating TGF-β superfamily signaling and cancer progression. Alterations in TGF-β superfamily signaling are common in colon cancer; however, the role of TβRIII has not been examined. Although TβRIII expression is frequently lost at the message and protein level in human cancers and suppresses cancer progression in these contexts, here we demonstrate that, in colon cancer, TβRIII messenger RNA expression is not significantly altered and TβRIII expression is more frequently increased at the protein level, suggesting a distinct role for TβRIII in colon cancer. Increasing TβRIII expression in colon cancer model systems enhanced ligand-mediated phosphorylation of p38 and the Smad proteins, while switching TGF-β and BMP-2 from inhibitors to stimulators of colon cancer cell proliferation, inhibiting ligand-induced p21 and p27 expression. In addition, increasing TβRIII expression increased ligand-stimulated anchorage-independent growth, a resistance to ligand- and chemotherapy-induced apoptosis, cell migration and modestly increased tumorigenicity in vivo. In a reciprocal manner, silencing endogenous TβRIII expression decreased colon cancer cell migration. These data support a model whereby TβRIII mediates TGF-β superfamily ligand-induced colon cancer progression and support a context-dependent role for TβRIII in regulating cancer progression. PMID:21847367

APELA promotes tumour growth and cell migration in ovarian cancer in a p53-dependent manner.

PubMed

Yi, Yuyin; Tsai, Shu-Huei; Cheng, Jung-Chien; Wang, Evan Y; Anglesio, Michael S; Cochrane, Dawn R; Fuller, Megan; Gibb, Ewan A; Wei, Wei; Huntsman, David G; Karsan, Aly; Hoodless, Pamela A

2017-12-01

APELA is a small, secreted peptide that can function as a ligand for the G-protein coupled receptor, Apelin Receptor (APLNR, APJ). APELA plays an essential role in endoderm differentiation and cardiac development during embryogenesis. We investigated whether APELA exerts any functions in cancer progression. The Cancer Genome Atlas (TCGA) RNA sequencing datasets, microarray from an OCCC mouse model, and RNA isolated from fresh frozen and FFPE patient tissue were used to assess APELA expression. APELA knockout ovarian clear cell carcinoma (OCCC) cell lines were generated using CRISPR/Cas9. APELA was expressed in various ovarian cancer histotypes and was especially elevated in OCCC. Disruption of APELA expression in OCCC cell lines suppressed cell growth and migration, and altered cell-cycle progression. Moreover, addition of human recombinant APELA peptide to the OCCC cell line OVISE promoted cell growth and migration. Interestingly, OVISE cells do not express APLNR, suggesting that APELA can function through an APLNR-independent pathway. Furthermore, APELA affected cell growth and cell cycle progression in a p53-dependent manner. In addition, APELA knockdown induced p53 expression in cancer cell lines. Our findings uncover a potential oncogenic role for APELA in promoting ovarian tumour progression and provide a possible therapeutic strategy in ovarian cancer by targeting APELA. Copyright © 2017 Elsevier Inc. All rights reserved.

Collagen triple helix repeat containing-1 promotes pancreatic cancer progression by regulating migration and adhesion of tumor cells.

PubMed

Park, Eun Hye; Kim, Seokho; Jo, Ji Yoon; Kim, Su Jin; Hwang, Yeonsil; Kim, Jin-Man; Song, Si Young; Lee, Dong-Ki; Koh, Sang Seok

2013-03-01

Collagen triple helix repeat containing-1 (CTHRC1) is a secreted protein involved in vascular remodeling, bone formation and developmental morphogenesis. CTHRC1 has recently been shown to be expressed in human cancers such as breast cancer and melanoma. In this study, we show that CTHRC1 is highly expressed in human pancreatic cancer tissues and plays a role in the progression and metastasis of the disease. CTHRC1 promoted primary tumor growth and metastatic spread of cancer cells to distant organs in orthotopic xenograft tumor mouse models. Overexpression of CTHRC1 in cancer cells resulted in increased motility and adhesiveness, whereas these cellular activities were diminished by down-regulation of the protein. CTHRC1 activated several key signaling molecules, including Src, focal adhesion kinase, paxillin, mitogen-activated protein kinase kinase (MEK), extracellular signal-regulated kinase and Rac1. Treatment with chemical inhibitors of Src, MEK or Rac1 and expression of dominant-negative Rac1 attenuated CTHRC1-induced cell migration and adhesion. Collectively, our results suggest that CTHRC1 has a role in pancreatic cancer progression and metastasis by regulating migration and adhesion activities of cancer cells.

Interim 2001-based national population projections for the United Kingdom and constituent countries.

PubMed

Shaw, Chris

2003-01-01

This article describes new 2001-based national population projections which were carried out following the publication in September 2002 of the first results of the 2001 Census. These "interim" projections, carried out by the Government Actuary in consultation with the Registrars General, take preliminary account of the results of the Census which showed that the base population used in previous projections was overestimated. The interim projections also incorporate a reduced assumption of net international migration to the United Kingdom, informed by the first results of the 2001 Census and taking account of more recent migration information. The population of the United Kingdom is now projected to increase from an estimated 58.8 million in 2001 to reach 63.2 million by 2026. The projected population at 2026 is about 1.8 million (2.8 per cent) lower than in the previous (2000-based) projections.

Turkey's Progress toward Meeting Refugee Education Needs the Example of Syrian Refugees

ERIC Educational Resources Information Center

Beltekin, Nurettin

2016-01-01

Problem Statement: Historically, Turkey is an immigrant country. It has experienced various migration waves from Asia, Awrupa and Africa. Recently, Turkey has confronted a huge wave of migration. Turkey tries to meet many needs besides the educational needs of refugees, but there is not enough study on refugees in the field of educational sciences…

BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain

PubMed Central

Teoh, Jia-Jie; Iwano, Tomohiko; Kunii, Masataka; Atik, Nur; Avriyanti, Erda; Yoshimura, Shin-ichiro; Moriwaki, Kenta

2017-01-01

BIG1, an activator protein of the small GTPase, Arf, and encoded by the Arfgef1 gene, is one of candidate genes for epileptic encephalopathy. To know the involvement of BIG1 in epileptic encephalopathy, we analyzed BIG1-deficient mice and found that BIG1 regulates neurite outgrowth and brain development in vitro and in vivo. The loss of BIG1 decreased the size of the neocortex and hippocampus. In BIG1-deficient mice, the neuronal progenitor cells (NPCs) and the interneurons were unaffected. However, Tbr1+ and Ctip2+ deep layer (DL) neurons showed spatial-temporal dependent apoptosis. This apoptosis gradually progressed from the piriform cortex (PIR), peaked in the neocortex, and then progressed into the hippocampus from embryonic day 13.5 (E13.5) to E17.5. The upper layer (UL) and DL order in the neocortex was maintained in BIG1-deficient mice, but the excitatory neurons tended to accumulate before their destination layers. Further pulse-chase migration assay showed that the migration defect was non-cell autonomous and secondary to the progression of apoptosis into the BIG1-deficient neocortex after E15.5. In BIG1-deficient mice, we observed an ectopic projection of corticothalamic axons from the primary somatosensory cortex (S1) into the dorsal lateral geniculate nucleus (dLGN). The thalamocortical axons were unable to cross the diencephalon–telencephalon boundary (DTB). In vitro, BIG1-deficient neurons showed a delay in neuronal polarization. BIG1-deficient neurons were also hypersensitive to low dose glutamate (5 μM), and died via apoptosis. This study showed the role of BIG1 in the survival of DL neurons in developing embryonic brain and in the generation of neuronal polarity. PMID:28414797

Knockdown of Tripartite-59 (TRIM59) Inhibits Cellular Proliferation and Migration in Human Cervical Cancer Cells.

PubMed

Aierken, Gulijiahan; Seyiti, Ayinuer; Alifu, Mayinuer; Kuerban, Gulina

2017-03-13

The tripartite motif (TRIM) family of proteins is a class of highly conservative proteins that have been implicated in multiple processes. TRIM59, one member of the TRIM family, has now received recognition as a key regulator in the development and progression of human diseases. However, its role in human tumorigenesis has remained largely unknown. In this study, the effects of TRIM59 expression on cell proliferation and migration were investigated in human cervical cancer cells. The expression of TRIM59 in clinical cervical cancer tissues and cervical cancer cells was initially determined by RT-PCR and Western blot. Specific shRNA against TRIM59 was then employed to knock down the expression of TRIM59 in cervical cancer lines HeLa and SiHa. The effects of TRIM59 knockdown on cell proliferation was assessed by MTT assay and colony formation assay. Transwell assay was conducted to reveal cell migration and invasion abilities before and after TRIM59 knockdown. Our results showed that the expression of TRIM59 was significantly elevated in cervical cancers. Knockdown of TRIM59 significantly inhibited cell proliferation and colony formation as well as cell migration and invasion abilities in cervical cancer HeLa and SiHa cells. Cell cycle progression analysis showed that TRIM59-depleted cells preferred to accumulate in the S phase. These data suggest that TRIM59 is a potential target that promotes the progression of cervical cancer.

Role of Rac1/WAVE2 Signaling in Mediating the Inhibitory Effects of γ-Tocotrienol on Mammary Cancer Cell Migration and Invasion.

PubMed

Algayadh, Ibrahim Gayadh; Dronamraju, Venkateshwararao; Sylvester, Paul William

2016-01-01

The majority of breast cancer deaths result from the progression of this disease to a metastatic phenotype. Rac1 and Cdc42 are Rho family members that together with their downstream effectors, Wiskott-Aldrich Syndrome protein-family verprolin-homologous protein 2 (WAVE2) and Arp2/3, play an important role in cytoskeletal reorganization and the formation of membrane protrusions that promote cancer cell migration and invasion. γ-Tocotrienol, is a natural isoform within the vitamin E family of compounds that inhibits breast cancer cell growth and progression by suppressing various signaling pathways involved in mitogenic signaling and metastatic progression. Studies were conducted to examine the effects of γ-tocotrienol on Rac1/WAVE2 signaling dependent migration and invasion in highly metastatic mouse +SA and human MDA-MB-231 mammary cancer cells. Exposure to γ-tocotrienol resulted in a dose-responsive decrease in Rac1/WAVE2 signaling as characterized by a suppression in the levels of Rac1/Cdc42, phospho-Rac1/Cdc42, WAVE2, Arp2, and Arp3 expression. Additional studies also demonstrated that similar treatment with γ-tocotrienol resulted in a significant reduction in tumor cell migration and invasion. Taken together, these findings indicate that γ-tocotrienol treatment effectively inhibits Rac1/WAVE2 signaling and reduces metastatic phenotypic expression in mammary cancer cells, suggesting that γ-tocotrienol may provide some benefit as a novel therapeutic approach in the treatment of metastatic breast cancer.

Decoupling migration effects from income effects on reproduction in Central American migrant-sending households.

PubMed

Davis, Jason

2011-01-01

Contemporary data for three Central American countries (Costa Rica, Guatemala, and Nicaragua) surveyed by the Latin American Migration Project were analyzed to determine if migration length and remittance transfers had an influence on fertility. The analysis was structured to separate societal influences on fertility attributable to migration from the income effects associated with remittance transfers. At the couple level, the odds that a birth would occur were negatively associated with an increase in U.S. remittance receipts and an increase in a wife’s migration duration. However, no correlation was found between length of male migration and couple fertility.

Prospects for the Comparative Study of International Migration using quasi-longitudinal micro-data

PubMed Central

Liu, Mao-Mei; Creighton, Mathew J.; Riosmena, Fernando; Baizán Mun͂oz, Pau

2017-01-01

BACKGROUND Longitudinal micro-level data about international migration behavior are notoriously difficult to collect, but data collection efforts have become more frequent in recent years. Comparative research of the patterns and processes of international migration, however, remains quite rare, especially that which compares across regions. OBJECTIVE We highlight the promises and difficulties of comparative international migration research, by offering a detailed comparison of two prominent data collection efforts. METHODS We systematically review existing sources of longitudinal and quasi-longitudinal individual-level and household-level data of international migration. We then compare two widely-used data sources: the Mexican Migration Project (MMP) and the Migration between Africa and Europe project (MAFE). RESULTS Data collection efforts are increasingly diverse, yet public accessibility of data remains limited. Also, comparability of data collected across settings can be complicated. In our MMP-MAFE analysis, we show some ways in which comparability can be achieved. CONCLUSIONS A primary roadblock to international comparative research is that, with some exceptions, the public accessibility of data remains low. Even when data is public and surveys are modeled after one another, comparability is not easy due to necessary trade-offs in adapting surveys to local settings and to developments in the field. CONTRIBUTION We demonstrate that, despite great strides in collecting quasi-longitudinal data of international migration, data accessibility still hinders the study of migration. With regards to comparability, our article provides important lessons for future data collection and analysis efforts that could improve comparability and thus advance understanding of the complex dynamics of international migration. PMID:29276429

Mangiferin inhibits cell migration and invasion through Rac1/WAVE2 signalling in breast cancer.

PubMed

Deng, Qing; Tian, Yan-Xiao; Liang, JianJun

2018-04-01

Breast tumour progression results from the advancement of the disease to a metastatic phenotype. Rac1 and Cdc42 belong to the Rho family of genes that, together with their downstream effectors, Wiskott-Aldrich Syndrome protein-family verprolin-homologous protein 2 (WAVE2) and Arp2/3, assume a vital part in cytoskeletal rearrangement and the arrangement of film projections that advance malignant cell relocation and invasion. Mangiferin is a characteristic polyphenolic compound from Mangifera indica L. (Anacardiaceae), ordinarily referred to as mango, that is consumed worldwide as a natural product, including culinary and seasoning applications. Mangiferin delays breast malignancy development and progression by inhibiting different signalling pathways required in mitogenic signalling and metastatic progression. Studies were performed to analyse the impact of mangiferin on Rac1/WAVE2 flagging, relocation and invasion in highly metastatic human MDA-MB-231 mammary cells. Additional studies led to the observation that comparative treatment with mangiferin caused marked reduction in tumour cell movement and invasion. Taken together, these discoveries demonstrate that mangiferin treatment adequately hinders Rac1/WAVE2 flagging and diminishes metastatic phenotypic expression in malignant mammary cells, indicating that mangiferin may provide a benefit as a novel restorative approach in the treatment of metastatic breast cancer.

Skilled craftswomen or cheap labour? Craft-based NGO projects as an alternative to female urban migration in northern Thailand.

PubMed

Humphreys, R

1999-07-01

This article presents a craft-based nongovernmental organization (NGO) project designed as an alternative to female urban migration in northern Thailand. ThaiCraft works with over 60 community-based artisan groups including members of minority and refugee groups. Activities include supporting community groups in their move toward self-reliance, coordination of producers' activities for ensuring fair payment, and maximizing marketing opportunities to increase producers' income. One of the project¿s aims is to form dynamic educational partnerships among producers, volunteers, and the public through the provision of training. Still, it is doubtful whether craft-based NGOs and other organizations can constitute a viable long-term alternative to urban migration. No proposed solution can respond to all issues associated with the emigration of rural Thai women for employment; however, the recognition of women's skills and knowledge is of importance to the fight against gender inequality in development.

Migration of health workers: a challenge for health care system.

PubMed

Afzal, Shaista; Masroor, Imrana; Shafqat, Gulnaz

2012-09-01

The migration of health workers has resulted in a growing apprehension universally because of its impact on health system of the developing countries. Although the choice to migrate is basically a personal one, however, the overall social and economic circumstances have important impact on the decision to migrate. The "push and pull" factors for migration are disparity in working conditions, pay, lack of promotion opportunities, poor living conditions, desire to gain experience, professional development, family background and family wealth. A strategic approach by the government and other agencies is mandatory for regulating the flow of health workers between countries. A range of policies and interventions are needed to deal with the broader health system issue and problems of health workers that influence their recruitment, retention, deployment and progress.

Paternal Absence and International Migration: Stressors and Compensators Associated with the Mental Health of Mexican Teenagers of Rural Origin

ERIC Educational Resources Information Center

Aguilera-Guzman, Rosa Maria; de Snyder, V. Nelly Salgado; Romero, Martha; Medina-Mora, Maria Elena

2004-01-01

Mexico-U.S. migration creates situations that may cause psychological distress. The purpose of this research project was to study the impact of father's physical absence due to international migration (FPAIM) on adolescent offspring of rural immigrants from Zacatecas, Mexico. Stressors and compensators were studied from the adolescent's…

3D printing of biomimetic microstructures for cancer cell migration.

PubMed

Huang, Tina Qing; Qu, Xin; Liu, Justin; Chen, Shaochen

2014-02-01

To understand the physical behavior and migration of cancer cells, a 3D in vitro micro-chip in hydrogel was created using 3D projection printing. The micro-chip has a honeycomb branched structure, aiming to mimic 3D vascular morphology to test, monitor, and analyze differences in the behavior of cancer cells (i.e. HeLa) vs. non-cancerous cell lines (i.e. 10 T1/2). The 3D Projection Printing system can fabricate complex structures in seconds from user-created designs. The fabricated microstructures have three different channel widths of 25, 45, and 120 microns wide to reflect a range of blood vessel diameters. HeLa and 10 T1/2 cells seeded within the micro-chip were then analyzed for morphology and cell migration speed. 10 T1/2 cells exhibited greater changes in morphology due to channel size width than HeLa cells; however, channel width had a limited effect on 10 T1/2 cell migration while HeLa cancer cell migration increased as channel width decreased. This physiologically relevant 3D cancer tissue model has the potential to be a powerful tool for future drug discoveries and cancer migration studies.

Floating Node Method and Virtual Crack Closure Technique for Modeling Matrix Cracking-Delamination Interaction

NASA Technical Reports Server (NTRS)

DeCarvalho, N. V.; Chen, B. Y.; Pinho, S. T.; Baiz, P. M.; Ratcliffe, J. G.; Tay, T. E.

2013-01-01

A novel approach is proposed for high-fidelity modeling of progressive damage and failure in composite materials that combines the Floating Node Method (FNM) and the Virtual Crack Closure Technique (VCCT) to represent multiple interacting failure mechanisms in a mesh-independent fashion. In this study, the approach is applied to the modeling of delamination migration in cross-ply tape laminates. Delamination, matrix cracking, and migration are all modeled using fracture mechanics based failure and migration criteria. The methodology proposed shows very good qualitative and quantitative agreement with experiments.

Long non-coding RNA BCAR4 promotes chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway

PubMed Central

Shui, Xiaolong; Zhou, Chengwei; Lin, Wei; Yu, Yang; Feng, Yongzeng

2017-01-01

Background: Chondrosarcoma is one of the common malignant histologic tumors, very difficult to treat, but the concrete cause and mechanism have not yet been elucidated. The present study aimed to investigate the functional involvement of BCAR4 in chondrosarcoma and its potentially underlying mechanism. QRT-PCR and western blot were used to determine the expression of BCAR4 and mTOR signaling pathway proteins both in chondrosarcoma tissues and cells. Chondrosarcoma cell proliferation and migration were assessed by MTT assay and transwell migration assay, respectively. The expression vectors were constructed and used to modulate the expression of BCAR4 and mTOR. Chondrosarcoma xenograft mouse model was established by subcutaneous injection with chondrosarcoma cell lines. The tumor volume was monitored to evaluate the effect of BCAR4 on chondrosarcoma cell tumorigenicity. The expressions of BCAR4, p-mTOR and p-P70S6K were up-regulated in chondrosarcoma tissues and cell lines. Moreover, BCAR4 overexpression had significant promoting effect on cell proliferation and migration in chondrosarcoma cells. Furthermore, mTOR signaling pathway was epigenetically activated by BCAR4-induced hyperacetylation of histone H3. We also found that mTOR overexpression abolished the decrease of chondrosarcoma cell proliferation and migration induced by BCAR4 knockdown. In vivo experiments confirmed that BCAR4 overexpression significantly accelerated tumor growth, while the knockdown of BCAR4 significantly inhibited tumor growth. BCAR4 promoted chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway, and thus contributed to chondrosarcoma progression. Impact statement LncRNA BCAR4 promoted chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway, and thus contributed to chondrosarcoma progression. PMID:28399646

Long non-coding RNA BCAR4 promotes chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway.

PubMed

Shui, Xiaolong; Zhou, Chengwei; Lin, Wei; Yu, Yang; Feng, Yongzeng; Kong, Jianzhong

2017-05-01

Chondrosarcoma is one of the common malignant histologic tumors, very difficult to treat, but the concrete cause and mechanism have not yet been elucidated. The present study aimed to investigate the functional involvement of BCAR4 in chondrosarcoma and its potentially underlying mechanism. QRT-PCR and western blot were used to determine the expression of BCAR4 and mTOR signaling pathway proteins both in chondrosarcoma tissues and cells. Chondrosarcoma cell proliferation and migration were assessed by MTT assay and transwell migration assay, respectively. The expression vectors were constructed and used to modulate the expression of BCAR4 and mTOR. Chondrosarcoma xenograft mouse model was established by subcutaneous injection with chondrosarcoma cell lines. The tumor volume was monitored to evaluate the effect of BCAR4 on chondrosarcoma cell tumorigenicity. The expressions of BCAR4, p-mTOR and p-P70S6K were up-regulated in chondrosarcoma tissues and cell lines. Moreover, BCAR4 overexpression had significant promoting effect on cell proliferation and migration in chondrosarcoma cells. Furthermore, mTOR signaling pathway was epigenetically activated by BCAR4-induced hyperacetylation of histone H3. We also found that mTOR overexpression abolished the decrease of chondrosarcoma cell proliferation and migration induced by BCAR4 knockdown. In vivo experiments confirmed that BCAR4 overexpression significantly accelerated tumor growth, while the knockdown of BCAR4 significantly inhibited tumor growth. BCAR4 promoted chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway, and thus contributed to chondrosarcoma progression. Impact statement LncRNA BCAR4 promoted chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway, and thus contributed to chondrosarcoma progression.

An in vitro correlation of mechanical forces and metastatic capacity

NASA Astrophysics Data System (ADS)

Indra, Indrajyoti; Undyala, Vishnu; Kandow, Casey; Thirumurthi, Umadevi; Dembo, Micah; Beningo, Karen A.

2011-02-01

Mechanical forces have a major influence on cell migration and are predicted to significantly impact cancer metastasis, yet this idea is currently poorly defined. In this study we have asked if changes in traction stress and migratory properties correlate with the metastatic progression of tumor cells. For this purpose, four murine breast cancer cell lines derived from the same primary tumor, but possessing increasing metastatic capacity, were tested for adhesion strength, traction stress, focal adhesion organization and for differential migration rates in two-dimensional and three-dimensional environments. Using traction force microscopy (TFM), we were surprised to find an inverse relationship between traction stress and metastatic capacity, such that force production decreased as the metastatic capacity increased. Consistent with this observation, adhesion strength exhibited an identical profile to the traction data. A count of adhesions indicated a general reduction in the number as metastatic capacity increased but no difference in the maturation as determined by the ratio of nascent to mature adhesions. These changes correlated well with a reduction in active beta-1 integrin with increasing metastatic ability. Finally, in two dimensions, wound healing, migration and persistence were relatively low in the entire panel, maintaining a downward trend with increasing metastatic capacity. Why metastatic cells would migrate so poorly prompted us to ask if the loss of adhesive parameters in the most metastatic cells indicated a switch to a less adhesive mode of migration that would only be detected in a three-dimensional environment. Indeed, in three-dimensional migration assays, the most metastatic cells now showed the greatest linear speed. We conclude that traction stress, adhesion strength and rate of migration do indeed change as tumor cells progress in metastatic capacity and do so in a dimension-sensitive manner.

Contact guidance is cell cycle-dependent.

PubMed

Pourfarhangi, Kamyar Esmaeili; De La Hoz, Edgar Cardenas; Cohen, Andrew R; Gligorijevic, Bojana

2018-09-01

Cancer cell migration is essential for metastasis, during which cancer cells move through the tumor and reach the blood vessels. In vivo , cancer cells are exposed to contact guidance and chemotactic cues. Depending on the strength of such cues, cells will migrate in a random or directed manner. While similar cues may also stimulate cell proliferation, it is not clear whether cell cycle progression affects migration of cancer cells and whether this effect is different in random versus directed migration. In this study, we tested the effect of cell cycle progression on contact guided migration in 2D and 3D environments, in the breast carcinoma cell line, FUCCI-MDA-MB-231. The results were quantified from live cell microscopy images using the open source lineage editing and validation image analysis tools (LEVER). In 2D, cells were placed inside 10 μ m-wide microchannels to stimulate contact guidance, with or without an additional chemotactic gradient of the soluble epidermal growth factor. In 3D, contact guidance was modeled by aligned collagen fibers. In both 2D and 3D, contact guidance was cell cycle-dependent, while the addition of the chemo-attractant gradient in 2D increased cell velocity and persistence in directionally migrating cells, regardless of their cell cycle phases. In both 2D and 3D contact guidance, cells in the G1 phase of the cell cycle outperformed cells in the S/G2 phase in terms of migration persistence and instantaneous velocity. These data suggest that in the presence of contact guidance cues in vivo , breast carcinoma cells in the G1 phase of the cell cycle may be more efficient in reaching the neighboring vasculature.

Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deng, Xuefeng, E-mail: dengxfdoctor@hotmail.com; Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences; Ma, Qunfeng

Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed to detect MSF mRNA and protein levels in tumor tissues and matched adjacent tumor-free tissues. Furthermore, to examine the effect of MSF on invasiveness, MSF was upregulated in A549 cells. The invasiveness and viability of A549 cells were then determined using a transwell migration assay and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assays, respectively. The expression level ofmore » MSF in NSCLC tissue was markedly higher than in matched adjacent tumor-free tissue. Additionally, the level of MSF protein expression in stage III and IV NSCLC samples was higher than in stage I and II NSCLC samples. More importantly, we also demonstrated that migration and invasion of A549 cells increased substantially after upregulating MSF, although proliferation remained unchanged. Meanwhile, we found no correlation between increasing motility and invasiveness of MSF-overexpressing cells and expression levels and activities of matrix metalloprotease MMP-2 and MMP-9. Our current study shows that MSF plays a role in migration and invasion of A549 cells and suggests that MSF may be a potential biomarker of NSCLC progression. - Highlights: • MSF expression was upregulated in NSCLC and correlated with TNM stages. • MSF may be a new biomarker for NSCLC progression. • MSF promoted migration and invasion in A549 cells, independent of MMP-2/MMP-9 expression.« less

Contribution of seismic processing to put up the scaffolding for the 3-dimensional study of deep sedimentary basins: the fundaments of trans-national 3D modelling in the project GeoMol

NASA Astrophysics Data System (ADS)

Capar, Laure

2013-04-01

Within the framework of the transnational project GeoMol geophysical and geological information on the entire Molasse Basin and on the Po Basin are gathered to build consistent cross-border 3D geological models based on borehole evidence and seismic data. Benefiting from important progress in seismic processing, these new models will provide some answers to various questions regarding the usage of subsurface resources, as there are geothermal energy, CO2 and gas storage, oil and gas production, and support decisions-making to national and local administrations as well as to industries. More than 28 000 km of 2D seismic lines are compiled reprocessed and harmonized. This work faces various problems like the vertical drop of more than 700 meters between West and East of the Molasse Basin and to al lesser extent in the Po Plain, the heterogeneities of the substratum, the large disparities between the period and parameters of seismic acquisition, and depending of their availability, the use of two types of seismic data, raw and processed seismic data. The main challenge is to harmonize all lines at the same reference level, amplitude and step of signal processing from France to Austria, spanning more than 1000 km, to avoid misfits at crossing points between seismic lines and artifacts at the country borders, facilitating the interpretation of the various geological layers in the Molasse Basin and Po Basin. A generalized stratigraphic column for the two basins is set up, representing all geological layers relevant to subsurface usage. This stratigraphy constitutes the harmonized framework for seismic reprocessing. In general, processed seismic data is available on paper at stack stage and the mandatory information to take these seismic lines to the final stage of processing, the migration step, are datum plane and replacement velocity. However several datum planes and replacement velocities were used during previous processing projects. Our processing sequence is to first digitize the data, to have them in SEG-Y format. The second step is to apply some post-stack processing to obtain a good data quality before the final migration step. The third step is the final migration, using optimized migration velocities and the fourth step is the post-migration processing. In case of raw seismic data, the mandatory information for processing is made accessible, like from observer logs, coordinates and field seismic data. The processing sequence in order to obtain the final usable version of the seismic line is based on a pre-stack time migration. A complex processing sequence is applied. One main issue is to deal with the significant changes in the topography along the seismic lines and in the first twenty meter layer, this low velocity zone (LVZ) or weathered zone, where some lateral velocity variations occur and disturb the wave propagation, therefore the seismic signal. In seismic processing, this matter is solved by using the static corrections which allow removing these effects of lateral velocity variations and the effects of topography. Another main item is the good determination of root mean square velocities for migration, to improve the final result of seismic processing. Within GeoMol, generalized 3D velocity models of stack velocities are calculated in order to perform a rapid time-depth conversion. In final, all seismic lines of the project GeoMol will be at the same level of processing, the migration level. But to tie all these lines, a single appropriate datum plane and replacement velocity for the entire Molasse Basin and Po Plain, respectively, have to be carefully set up, to avoid misties at crossing points. The reprocessing and use of these 28 000 km of seismic lines in the project GeoMol provide the pivotal database to build a 3D framework model for regional subsurface information on the Alpine foreland basins (cf. Rupf et al. 2013, EGU2013-8924). The project GeoMol is co-funded by the Alpine Space Program as part of the European Territorial Cooperation 2007-2013. The project integrates partners from Austria, France, Germany, Italy, Slovenia and Switzerland and runs from September 2012 to June 2015. Further information on www.geomol.eu The GeoMol seismic interpretation team: Roland Baumberger (swisstopo), Agnès BRENOT (BRGM), Alessandro CAGNONI (RLB), Renaud COUËFFE (BRGM), Gabriel COURRIOUX (BRGM), Chiara D'Ambrogi (ISPRA), Chrystel Dezayes (BRGM), Charlotte Fehn (LGRB), Sunseare GABALDA (BRGM), Gregor Götzl (GBA), Andrej Lapanje (GeoZS), Stéphane MARC (BRGM), Alberto MARTINI (RER-SGSS), Fabio Carlo Molinari (RER-SGSS), Edgar Nitsch (LGRB), Robert Pamer (LfU BY), Marco PANTALONI (ISPRA), Sebastian Pfleiderer (GBA), Andrea PICCIN (RLB), (Nils Oesterling (swisstopo), Isabel Rupf (LGRB), Uta Schulz (LfU BY), Yves SIMEON (BRGM), Günter SÖKOL (LGRB), Heiko Zumsprekel (LGRB)

How climate, migration ability and habitat fragmentation affect the projected future distribution of European beech.

PubMed

Saltré, Frédérik; Duputié, Anne; Gaucherel, Cédric; Chuine, Isabelle

2015-02-01

Recent efforts to incorporate migration processes into species distribution models (SDMs) are allowing assessments of whether species are likely to be able to track their future climate optimum and the possible causes of failing to do so. Here, we projected the range shift of European beech over the 21st century using a process-based SDM coupled to a phenomenological migration model accounting for population dynamics, according to two climate change scenarios and one land use change scenario. Our model predicts that the climatically suitable habitat for European beech will shift north-eastward and upward mainly because (i) higher temperature and precipitation, at the northern range margins, will increase survival and fruit maturation success, while (ii) lower precipitations and higher winter temperature, at the southern range margins, will increase drought mortality and prevent bud dormancy breaking. Beech colonization rate of newly climatically suitable habitats in 2100 is projected to be very low (1-2% of the newly suitable habitats colonised). Unexpectedly, the projected realized contraction rate was higher than the projected potential contraction rate. As a result, the realized distribution of beech is projected to strongly contract by 2100 (by 36-61%) mainly due to a substantial increase in climate variability after 2050, which generates local extinctions, even at the core of the distribution, the frequency of which prevents beech recolonization during more favourable years. Although European beech will be able to persist in some parts of the trailing edge of its distribution, the combined effects of climate and land use changes, limited migration ability, and a slow life-history are likely to increase its threat status in the near future. © 2014 John Wiley & Sons Ltd.

Domestic and International Climate Migration from Rural Mexico

PubMed Central

Nawrotzki, Raphael J.; Runfola, Daniel M.; Hunter, Lori M.; Riosmena, Fernando

2016-01-01

Evidence is increasing that climate change and variability may influence human migration patterns. However, there is less agreement regarding the type of migration streams most strongly impacted. This study tests whether climate change more strongly impacted international compared to domestic migration from rural Mexico during 1986-99. We employ eight temperature and precipitation-based climate change indices linked to detailed migration histories obtained from the Mexican Migration Project. Results from multilevel discrete-time event-history models challenge the assumption that climate-related migration will be predominantly short distance and domestic, but instead show that climate change more strongly impacted international moves from rural Mexico. The stronger climate impact on international migration may be explained by the self-insurance function of international migration, the presence of strong migrant networks, and climate-related changes in wage difference. While a warming in temperature increased international outmigration, higher levels of precipitation declined the odds of an international move. PMID:28439146

Domestic and International Climate Migration from Rural Mexico.

PubMed

Nawrotzki, Raphael J; Runfola, Daniel M; Hunter, Lori M; Riosmena, Fernando

2016-12-01

Evidence is increasing that climate change and variability may influence human migration patterns. However, there is less agreement regarding the type of migration streams most strongly impacted. This study tests whether climate change more strongly impacted international compared to domestic migration from rural Mexico during 1986-99. We employ eight temperature and precipitation-based climate change indices linked to detailed migration histories obtained from the Mexican Migration Project. Results from multilevel discrete-time event-history models challenge the assumption that climate-related migration will be predominantly short distance and domestic, but instead show that climate change more strongly impacted international moves from rural Mexico. The stronger climate impact on international migration may be explained by the self-insurance function of international migration, the presence of strong migrant networks, and climate-related changes in wage difference. While a warming in temperature increased international outmigration, higher levels of precipitation declined the odds of an international move.

A PDMS Device Coupled with Culture Dish for In Vitro Cell Migration Assay.

PubMed

Lv, Xiaoqing; Geng, Zhaoxin; Fan, Zhiyuan; Wang, Shicai; Pei, WeiHua; Chen, Hongda

2018-04-30

Cell migration and invasion are important factors during tumor progression and metastasis. Wound-healing assay and the Boyden chamber assay are efficient tools to investigate tumor development because both of them could be applied to measure cell migration rate. Therefore, a simple and integrated polydimethylsiloxane (PDMS) device was developed for cell migration assay, which could perform quantitative evaluation of cell migration behaviors, especially for the wound-healing assay. The integrated device was composed of three units, which included cell culture dish, PDMS chamber, and wound generation mold. The PDMS chamber was integrated with cell culture chamber and could perform six experiments under different conditions of stimuli simultaneously. To verify the function of this device, it was utilized to explore the tumor cell migration behaviors under different concentrations of fetal bovine serum (FBS) and transforming growth factor (TGF-β) at different time points. This device has the unique capability to create the "wound" area in parallel during cell migration assay and provides a simple and efficient platform for investigating cell migration assay in biomedical application.

RhoA and RhoC are involved in stromal cell-derived factor-1-induced cell migration by regulating F-actin redistribution and assembly.

PubMed

Luo, Jixian; Li, Dingyun; Wei, Dan; Wang, Xiaoguang; Wang, Lan; Zeng, Xianlu

2017-12-01

Stromal cell-derived factor-1 (SDF-1) signaling is important to the maintenance and progression of T-cell acute lymphoblastic leukemia by inducing chemotaxis migration. To identify the mechanism of SDF-1 signaling in the migration of T-ALL, Jurkat acute lymphoblastic leukemia cells were used. Results showed that SDF-1 induces Jurkat cell migration by F-actin redistribution and assembly, which is dependent on Rho activity. SDF-1 induced RhoA and RhoC activation, as well as reactive oxygen species (ROS) production, which was inhibited by Rho inhibitor. The Rho-dependent ROS production led to subsequent cytoskeleton redistribution and assembly in the process of migration. Additionally, RhoA and RhoC were involved in SDF-1-induced Jurkat cell migration. Taken together, we found a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. This work will contribute to a clearer insight into the migration mechanism of acute lymphoblastic leukemia.

Effect of TRPV2 cation channels on the proliferation, migration and invasion of 5637 bladder cancer cells.

PubMed

Liu, Quanliang; Wang, Xinghuan

2013-11-01

Transient receptor potential vanilloid 2 (TRPV2), a nonselective cation channel, has become an attractive target gene for tumor studies due to its wide range of physiological and pathological functions. However, its specific role in bladder cancer development and progression remains unclear. The aim of the present study was to investigate the effects of TRPV2 on the proliferation, migration and invasion of 5637 bladder cancer cells in vitro . Rat TRPV2 cDNA was transfected into 5637 bladder cancer cells and changes in the behavior of the cells were detected. It was observed that TRPV2 enhanced bladder cancer cell migration and invasion; however, it did not affect cell proliferation in vitro . TRPV2 activity, which may be mediated by direct matrix metalloproteinase 2 (MMP2) regulation, is important in bladder tumor development and progression. The results of this study suggest that TRPV2 channels are a potential therapeutic target for bladder carcinoma.

Effect of TRPV2 cation channels on the proliferation, migration and invasion of 5637 bladder cancer cells

PubMed Central

LIU, QUANLIANG; WANG, XINGHUAN

2013-01-01

Transient receptor potential vanilloid 2 (TRPV2), a nonselective cation channel, has become an attractive target gene for tumor studies due to its wide range of physiological and pathological functions. However, its specific role in bladder cancer development and progression remains unclear. The aim of the present study was to investigate the effects of TRPV2 on the proliferation, migration and invasion of 5637 bladder cancer cells in vitro. Rat TRPV2 cDNA was transfected into 5637 bladder cancer cells and changes in the behavior of the cells were detected. It was observed that TRPV2 enhanced bladder cancer cell migration and invasion; however, it did not affect cell proliferation in vitro. TRPV2 activity, which may be mediated by direct matrix metalloproteinase 2 (MMP2) regulation, is important in bladder tumor development and progression. The results of this study suggest that TRPV2 channels are a potential therapeutic target for bladder carcinoma. PMID:24223658

Oral administration of FAK inhibitor TAE226 inhibits the progression of peritoneal dissemination of colorectal cancer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hao, Hui-fang; Takaoka, Munenori; Bao, Xiao-hong

2012-07-13

Highlights: Black-Right-Pointing-Pointer A novel FAK inhibitor TAE226 suppressed FAK activity in HCT116 colon cancer cells. Black-Right-Pointing-Pointer TAE226 suppressed proliferation and migration, with a modest effect on adhesion. Black-Right-Pointing-Pointer Silencing of FAK by siRNA made no obvious difference on cancer cell attachment. Black-Right-Pointing-Pointer TAE226 treatment suppressed the progression of peritoneal dissemination. Black-Right-Pointing-Pointer Oral administration of TAE226 prolonged the survival of tumor-bearing mice. -- Abstract: Peritoneal dissemination is one of the most terrible types of colorectal cancer progression. Focal adhesion kinase (FAK) plays a crucial role in the biological processes of cancer, such as cell attachment, migration, proliferation and survival, all ofmore » which are essential for the progression of peritoneal dissemination. Since we and other groups have reported that the inhibition of FAK activity exhibited a potent anticancer effect in several cancer models, we hypothesized that TAE226, a novel ATP-competitive tyrosine kinase inhibitor designed to target FAK, can prevent the occurrence and progression of peritoneal dissemination. In vitro, TAE226 greatly inhibited the proliferation and migration of HCT116 colon cancer cells, while their adhesion on the matrix surface was minimally inhibited when FAK activity and expression was suppressed by TAE226 and siRNA. In vivo, when HCT116 cells were intraperitoneally inoculated in mice, the cells could attach to the peritoneum and begin to grow within 24 h regardless of the pretreatment of cells with TAE226 or FAK-siRNA, suggesting that FAK is not essential, at least for the initial integrin-matrix contact. Interestingly, the treatment of mice before and after inoculation significantly suppressed cell attachment to the peritoneum. Furthermore, oral administration of TAE226 greatly reduced the size of disseminated tumors and prolonged survival in tumor-bearing mice. Taken together, a possible strategy for inhibiting peritoneal dissemination by targeting FAK with TAE226 appears to be applicable through anti-proliferative and anti-invasion/anti-migration mechanisms.« less

The MUC4 membrane-bound mucin regulates esophageal cancer cell proliferation and migration properties: Implication for S100A4 protein.

PubMed

Bruyère, Emilie; Jonckheere, Nicolas; Frénois, Frédéric; Mariette, Christophe; Van Seuningen, Isabelle

2011-09-23

MUC4 is a membrane-bound mucin known to participate in tumor progression. It has been shown that MUC4 pattern of expression is modified during esophageal carcinogenesis, with a progressive increase from metaplastic lesions to adenocarcinoma. The principal cause of development of esophageal adenocarcinoma is the gastro-esophageal reflux, and MUC4 was previously shown to be upregulated by several bile acids present in reflux. In this report, our aim was thus to determine whether MUC4 plays a role in biological properties of human esophageal cancer cells. For that stable MUC4-deficient cancer cell lines (shMUC4 cells) were established using a shRNA approach. In vitro (proliferation, migration and invasion) and in vivo (tumor growth following subcutaneous xenografts in SCID mice) biological properties of shMUC4 cells were analyzed. Our results show that shMUC4 cells were less proliferative, had decreased migration properties and did not express S100A4 protein when compared with MUC4 expressing cells. Absence of MUC4 did not impair shMUC4 invasiveness. Subcutaneous xenografts showed a significant decrease in tumor size when cells did not express MUC4. Altogether, these data indicate that MUC4 plays a key role in proliferative and migrating properties of esophageal cancer cells as well as is a tumor growth promoter. MUC4 mucin appears thus as a good therapeutic target to slow-down esophageal tumor progression. Copyright © 2011 Elsevier Inc. All rights reserved.

Polyethylene damage and deformation on fixed-bearing, non-conforming unicondylar knee replacements corresponding to progressive changes in alignment and fixation.

PubMed

Harman, Melinda K; Schmitt, Sabine; Rössing, Sven; Banks, Scott A; Sharf, Hans-Peter; Viceconti, Marco; Hodge, W Andrew

2010-07-01

Deviations from nominal alignment of unicondylar knee replacements impact knee biomechanics, including the load and stress distribution at the articular contact surfaces. This study characterizes relationships between the biomechanical environment, distinguished by progressive changes in alignment and fixation, and articular damage and deformation in a consecutive series of retrieved unicondylar knee replacements. Twenty seven fixed-bearing, non-conforming unicondylar knee replacements of one design were retrieved after 2 to 13 years of in vivo function. The in vivo biomechanical environment was characterized by grading component migration measured from full-length radiographs and grading component fixation based on intraoperative manual palpation. Articular damage patterns and linear deformation on the polyethylene inserts were measured using optical photogrammetry and contact point digitization. Articular damage patterns and surface deformation on the explanted polyethylene inserts corresponded to progressive changes in component alignment and fixation. Component migration produced higher deformation rates, whereas loosening contributed to larger damage areas but lower deformation rates. Migration and loosening of the femoral component, but not the tibial component, were factors contributing to large regions of abrasion concentrated on the articular periphery. Classifying component migration and fixation at revision proved useful for distinguishing common biomechanical conditions associated with the varied polyethylene damage patterns and linear deformation for this fixed-bearing, non-conforming design. Pre-clinical evaluations of unicondylar knee replacements that are capable of reproducing variations in clinical alignment and predicting the observed wear mechanisms are necessary to better understand the impact of knee biomechanics and design on unicondylar knee replacement longevity. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Disruption of Information Technology Projects: The Reactive Decoupling of Project Management Methodologies

ERIC Educational Resources Information Center

Schmitz, Kurt W.

2013-01-01

Information Technology projects have migrated toward two dominant Project Management (PM) methodologies. Plan-driven practices provide organizational control through highly structured plans, schedules, and specifications that facilitate oversight by hierarchical bureaucracies. In contrast, agile practices emphasize empowered teams using flexible…

Pregnancy associated plasma protein-A links pregnancy and melanoma progression by promoting cellular migration and invasion

PubMed Central

Prithviraj, Prashanth; Anaka, Matthew; McKeown, Sonja J; Permezel, Michael; Walkiewicz, Marzena

2015-01-01

Melanoma is the most common cancer diagnosed in pregnant women and an aggressive course with poorer outcomes is commonly described during pregnancy or shortly after childbirth. The underlying mechanisms for this are not understood. Here, we report that melanoma migration, invasiveness and progression are promoted by pregnancy-associated plasma protein-A (PAPPA), a pregnancy-associated metalloproteinase produced by the placenta that increases the bioavailability of IGF1 by cleaving it from a circulating complex formed with IGFBP4. We show that PAPPA is widely expressed by metastatic melanoma tumors and is elevated in melanoma cells exhibiting mesenchymal, invasive and label-retaining phenotypes. Notably, inhibition of PAPPA significantly reduced invasion and migration of melanoma cells in vitro and in vivo within the embryonic chicken neural tube. PAPPA-enriched pregnancy serum treatment enhanced melanoma motility in vitro. Furthermore, we report that IGF1 can induce the phenotypic and functional effects of epithelial-to-mesenchymal transition (EMT) in melanoma cells. In this study, we establish a clear relationship between a pregnancy-associated protein PAPPA, melanoma and functional effects mediated through IGF1 that provides a plausible mechanism for accelerated melanoma progression during pregnancy. This opens the possibility of targeting the PAPPA/IGF1 axis therapeutically. PMID:25940796

Pregnancy associated plasma protein-A links pregnancy and melanoma progression by promoting cellular migration and invasion.

PubMed

Prithviraj, Prashanth; Anaka, Matthew; McKeown, Sonja J; Permezel, Michael; Walkiewicz, Marzena; Cebon, Jonathan; Behren, Andreas; Jayachandran, Aparna

2015-06-30

Melanoma is the most common cancer diagnosed in pregnant women and an aggressive course with poorer outcomes is commonly described during pregnancy or shortly after childbirth. The underlying mechanisms for this are not understood. Here, we report that melanoma migration, invasiveness and progression are promoted by Pregnancy-Associated Plasma Protein-A (PAPPA), a pregnancy-associated metalloproteinase produced by the placenta that increases the bioavailability of IGF1 by cleaving it from a circulating complex formed with IGFBP4. We show that PAPPA is widely expressed by metastatic melanoma tumors and is elevated in melanoma cells exhibiting mesenchymal, invasive and label-retaining phenotypes. Notably, inhibition of PAPPA significantly reduced invasion and migration of melanoma cells in vitro and in vivo within the embryonic chicken neural tube. PAPPA-enriched pregnancy serum treatment enhanced melanoma motility in vitro. Furthermore, we report that IGF1 can induce the phenotypic and functional effects of epithelial-to-mesenchymal transition (EMT) in melanoma cells. In this study, we establish a clear relationship between a pregnancy-associated protein PAPPA, melanoma and functional effects mediated through IGF1 that provides a plausible mechanism for accelerated melanoma progression during pregnancy. This opens the possibility of targeting the PAPPA/IGF1 axis therapeutically.

Relational Data Bases--Are You Ready?

ERIC Educational Resources Information Center

Marshall, Dorothy M.

1989-01-01

Migrating from a traditional to a relational database technology requires more than traditional project management techniques. An overview of what to consider before migrating to relational database technology is presented. Leadership, staffing, vendor support, hardware, software, and application development are discussed. (MLW)

Control of Subsurface Contaminant Migration by Vertical Engineered Barriers

EPA Science Inventory

This Fact Sheet is intended to provide remedial project managers (RPMs), on-scene coordinators (OSCs), contractors, and other remediation stakeholders with a basic overview of hazardous waste containment systems constructed to prevent or limit the migration of contamination in gr...

S100A8 and S100A9 Are Associated with Colorectal Carcinoma Progression and Contribute to Colorectal Carcinoma Cell Survival and Migration via Wnt/β-Catenin Pathway

PubMed Central

Duan, Liang; Wu, Rui; Ye, Liwei; Wang, Haiyan; Yang, Xia; Zhang, Yunyuan; Chen, Xian; Zuo, Guowei; Zhang, Yan; Weng, Yaguang; Luo, Jinyong; Tang, Min; Shi, Qiong; He, Tongchuan; Zhou, Lan

2013-01-01

Background and Objective S100A8 and S100A9, two members of the S100 protein family, have been reported in association with the tumor cell differentiation and tumor progression. Previous study has showed that their expression in stromal cells of colorectal carcinoma (CRC) is associated with tumor size. Here, we investigated the clinical significances of S100A8 and S100A9 in tumor cells of CRC and their underlying molecular mechanisms. Methods Expression of S100A8 and S100A9 in colorectal carcinoma and matching distal normal tissues were measured by reverse transcriptase polymerase chain reaction (RT-PCR), immunohistochemistry and western blot. CRC cell lines treated with the recombinant S100A8 and S100A9 proteins were used to analyze the roles and molecular mechanisms of the two proteins in CRC in vitro. Results S100A8 and S100A9 were elevated in more than 50% of CRC tissues and their expression in tumor cells was associated with differentiation, Dukes stage and lymph node metastasis. The CRC cell lines treatment with recombinant S100A8 and S100A9 proteins promoted the viability and migration of CRC cells. Furthermore, the two recombinant proteins also resulted in the increased levels of β-catenin and its target genes c-myc and MMP7. β-catenin over-expression in CRC cells by Adβ-catenin increased cell viability and migration. β-catenin knock-down by Adsiβ-catenin reduced cell viability and migration. Furthermore, β-catenin knockdown also partially abolished the promotive effects of recombinant S100A8 and S100A9 proteins on the viability and migration of CRC cells. Conclusions Our work demonstrated that S100A8 and S100A9 are linked to the CRC progression, and one of the underlying molecular mechanisms is that extracellular S100A8 and S100A9 proteins contribute to colorectal carcinoma cell survival and migration via Wnt/β-catenin pathway. PMID:23637971

West Valley demonstration project: Implementation of the kerosene mitigation plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blickwedehl, R.R.; Goodman, J.; Valenti, P.J.

1987-05-01

An aggressive program was implemented to mitigate the migration of radioactive kerosene believed to have originated from the West Valley NRC-Licensed Disposal Area (NDA) disposal trenches designated as SH-10 and SH-11 (Special Holes 10 and 11). This report provides a historical background of the events leading to the migration problem, the results of a detailed investigation to determine the location and source of the kerosene migration, the remediation plan to mitigate the migration, and the actions taken to successfully stabilize the kerosene. 7 refs., 19 figs., 1 tab.

Out-migration and depopulation of the Russian North during the 1990s.

PubMed

Heleniak, T

1999-01-01

The large-scale out-migration from Russia's northern regions that has taken place over the course of the 1990s is analyzed. "The study is based on unpublished oblast-level migration data compiled by the Russian Government, field work by the author, as well as two extensive 1998 surveys of recent and potential migrants, respectively. Age, gender, and educational level of migrants are analyzed to determine the extent of change in Northern population structure attributable to migration. A concluding section presents Russian Government projections of the North's population to 2010." excerpt

TAFE Projects in Progress. No. 1.

ERIC Educational Resources Information Center

Skott, Diana, Comp.

1982-01-01

This publication contains a listing of educational research and development projects currently in progress in Australia, which is the first of a planned twice-yearly listing of projects in progress. Projects are listed by territories and are indexed by author and by subject. Information supplied for each project includes a reference number,…

High expression of hexokinase domain containing 1 is associated with poor prognosis and aggressive phenotype in hepatocarcinoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Zijian; Huang, Shanzhou; Wang, Huanyu

Rapid progress and metastasis remain the major treatment failure modes of hepatocarcinoma (HCC). Unfortunately, the underlying molecular mechanisms of hepatoma cell proliferation and migration are poorly understood. Metabolic abnormalities play critical roles in tumorigenesis and progression. Hexokinase domain containing 1 (HKDC1) catalyzes the phosphorylation of glucose. However, the functions and mechanisms of HKDC1 in cancer remain unknown. In this study, real-time RT-PCR and Western blotting assays were used to detect the HKDC1 expression levels in HCC tissues and cell lines. The Oncomine™ Cancer Microarray Database was applied to analysis the correlations between HKDC1 expression and HCC clinical characteristics. MTT andmore » Transwell migration assays were performed to determine the functions of HKDC1 in HCC cells. The effect of HKDC1 on Wnt/β-catenin signaling pathway was assessed using Western blotting assay. In this study, we found that HKDC1 expression levels were elevated in HCC tissues compared with the adjacent tissues. HCC patients with high expression levels of HKDC1 had poor overall survival (OS). Furthermore, higher HKDC1 levels also predicted a worse OS of patients within solitary, elevated pre-operated serum alpha fetoprotein (AFP) level and higher tumor diameter. Moreover, silencing HKDC1 suppressed HCC cells proliferation and migration in vitro. Downregulated HKDC1 expression repressed β-Catenin and c-Myc expression, which indicates that silencing HKDC1 may reduce proliferation and migration via inhibiting the Wnt/β-catenin signaling pathway in HCC. In summary, HKDC1 provides further insight into HCC tumor progression and may provide a novel prognostic biomarker and therapeutic target for HCC treatment. -- Highlights: •HKDC1 is upregulated in HCC. •Patients with high HKDC1 expressions perform worse OS. •Silencing HKDC1 suppresses proliferation and migration. •Silencing HKDC1 represses Wnt/β-catenin signaling pathway.« less

Breaking barriers through collaboration: the example of the Cell Migration Consortium.

PubMed

Horwitz, Alan Rick; Watson, Nikki; Parsons, J Thomas

2002-10-15

Understanding complex integrated biological processes, such as cell migration, requires interdisciplinary approaches. The Cell Migration Consortium, funded by a Large-Scale Collaborative Project Award from the National Institute of General Medical Science, develops and disseminates new technologies, data, reagents, and shared information to a wide audience. The development and operation of this Consortium may provide useful insights for those who plan similarly large-scale, interdisciplinary approaches.

The Old-Age Healthy Dependency Ratio in Europe.

PubMed

Muszyńska, Magdalena M; Rau, Roland

2012-09-01

The aim of this study is to answer the question of whether improvements in the health of the elderly in European countries could compensate for population ageing on the supply side of the labour market. We propose a state-of-health-specific (additive) decomposition of the old-age dependency ratio into an old-age healthy dependency ratio and an old-age unhealthy dependency ratio in order to participate in a discussion of the significance of changes in population health to compensate for the ageing of the labour force. Applying the proposed indicators to the Eurostat's population projection for the years 2010-2050, and assuming there will be equal improvements in life expectancy and healthy life expectancy at birth, we discuss various scenarios concerning future of the European labour force. While improvements in population health are anticipated during the years 2010-2050, the growth in the number of elderly people in Europe may be expected to lead to a rise in both healthy and unhealthy dependency ratios. The healthy dependency ratio is, however, projected to make up the greater part of the old-age dependency ratio. In the European countries in 2006, the value of the old-age dependency ratio was 25. But in the year 2050, with a positive migration balance over the years 2010-2050, there would be 18 elderly people in poor health plus 34 in good health per 100 people in the current working age range of 15-64. In the scenarios developed in this study, we demonstrate that improvements in health and progress in preventing disability will not, by themselves, compensate for the ageing of the workforce. However, coupled with a positive migration balance, at the level and with the age structure assumed in the Eurostat's population projections, these developments could ease the effect of population ageing on the supply side of the European labour market.

Hybrid Cloud Computing Environment for EarthCube and Geoscience Community

NASA Astrophysics Data System (ADS)

Yang, C. P.; Qin, H.

2016-12-01

The NSF EarthCube Integration and Test Environment (ECITE) has built a hybrid cloud computing environment to provides cloud resources from private cloud environments by using cloud system software - OpenStack and Eucalyptus, and also manages public cloud - Amazon Web Service that allow resource synchronizing and bursting between private and public cloud. On ECITE hybrid cloud platform, EarthCube and geoscience community can deploy and manage the applications by using base virtual machine images or customized virtual machines, analyze big datasets by using virtual clusters, and real-time monitor the virtual resource usage on the cloud. Currently, a number of EarthCube projects have deployed or started migrating their projects to this platform, such as CHORDS, BCube, CINERGI, OntoSoft, and some other EarthCube building blocks. To accomplish the deployment or migration, administrator of ECITE hybrid cloud platform prepares the specific needs (e.g. images, port numbers, usable cloud capacity, etc.) of each project in advance base on the communications between ECITE and participant projects, and then the scientists or IT technicians in those projects launch one or multiple virtual machines, access the virtual machine(s) to set up computing environment if need be, and migrate their codes, documents or data without caring about the heterogeneity in structure and operations among different cloud platforms.

Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia.

PubMed

Windus, Louisa C E; Claxton, Christina; Allen, Chelsea L; Key, Brian; St John, James A

2007-12-01

Olfactory ensheathing cells (OECs) are candidates for therapeutic approaches for neural regeneration due to their ability to assist axon regrowth in central nervous system lesion models. However, little is understood about the processes and mechanisms underlying migration of these cells. We report here that novel lamellipodial protrusions, termed lamellipodial waves, are integral to OEC migration. Time-lapse imaging of migrating OECs revealed that these highly dynamic waves progress along the shaft of the cells and are crucial for mediating cell-cell adhesion. Without these waves, cell-cell adhesion does not occur and migrational rates decline. The activity of waves is modulated by both glial cell line-derived neurotrophic factor and inhibitors of the JNK and SRC kinases. Furthermore, the activity of lamellipodial waves can be modulated by Mek1, independently of leading edge activity. The ability to selectively regulate cell migration via lamellipodial waves has implications for manipulating the migratory behavior of OECs during neural repair. (c) 2007 Wiley-Liss, Inc.

Cancer-associated fibroblasts promote directional cancer cell migration by aligning fibronectin.

PubMed

Erdogan, Begum; Ao, Mingfang; White, Lauren M; Means, Anna L; Brewer, Bryson M; Yang, Lijie; Washington, M Kay; Shi, Chanjuan; Franco, Omar E; Weaver, Alissa M; Hayward, Simon W; Li, Deyu; Webb, Donna J

2017-11-06

Cancer-associated fibroblasts (CAFs) are major components of the carcinoma microenvironment that promote tumor progression. However, the mechanisms by which CAFs regulate cancer cell migration are poorly understood. In this study, we show that fibronectin (Fn) assembled by CAFs mediates CAF-cancer cell association and directional migration. Compared with normal fibroblasts, CAFs produce an Fn-rich extracellular matrix with anisotropic fiber orientation, which guides the cancer cells to migrate directionally. CAFs align the Fn matrix by increasing nonmuscle myosin II- and platelet-derived growth factor receptor α-mediated contractility and traction forces, which are transduced to Fn through α5β1 integrin. We further show that prostate cancer cells use αv integrin to migrate efficiently and directionally on CAF-derived matrices. We demonstrate that aligned Fn is a prominent feature of invasion sites in human prostatic and pancreatic carcinoma samples. Collectively, we present a new mechanism by which CAFs organize the Fn matrix and promote directional cancer cell migration. © 2017 Erdogan et al.

Cancer-associated fibroblasts promote directional cancer cell migration by aligning fibronectin

PubMed Central

Ao, Mingfang; White, Lauren M.; Means, Anna L.; Yang, Lijie; Washington, M. Kay; Franco, Omar E.; Li, Deyu; Webb, Donna J.

2017-01-01

Cancer-associated fibroblasts (CAFs) are major components of the carcinoma microenvironment that promote tumor progression. However, the mechanisms by which CAFs regulate cancer cell migration are poorly understood. In this study, we show that fibronectin (Fn) assembled by CAFs mediates CAF–cancer cell association and directional migration. Compared with normal fibroblasts, CAFs produce an Fn-rich extracellular matrix with anisotropic fiber orientation, which guides the cancer cells to migrate directionally. CAFs align the Fn matrix by increasing nonmuscle myosin II- and platelet-derived growth factor receptor α–mediated contractility and traction forces, which are transduced to Fn through α5β1 integrin. We further show that prostate cancer cells use αv integrin to migrate efficiently and directionally on CAF-derived matrices. We demonstrate that aligned Fn is a prominent feature of invasion sites in human prostatic and pancreatic carcinoma samples. Collectively, we present a new mechanism by which CAFs organize the Fn matrix and promote directional cancer cell migration. PMID:29021221

A Glycosylation Mutant of Trypanosoma brucei Links Social Motility Defects In Vitro to Impaired Colonization of Tsetse Flies In Vivo.

PubMed

Imhof, Simon; Vu, Xuan Lan; Bütikofer, Peter; Roditi, Isabel

2015-06-01

Transmission of African trypanosomes by tsetse flies requires that the parasites migrate out of the midgut lumen and colonize the ectoperitrophic space. Early procyclic culture forms correspond to trypanosomes in the lumen; on agarose plates they exhibit social motility, migrating en masse as radial projections from an inoculation site. We show that an Rft1(-/-) mutant needs to reach a greater threshold number before migration begins, and that it forms fewer projections than its wild-type parent. The mutant is also up to 4 times less efficient at establishing midgut infections. Ectopic expression of Rft1 rescues social motility defects and restores the ability to colonize the fly. These results are consistent with social motility reflecting movement to the ectoperitrophic space, implicate N-glycans in the signaling cascades for migration in vivo and in vitro, and provide the first evidence that parasite-parasite interactions determine the success of transmission by the insect host. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Assessment of smolt condition: Biological and environmental interactions -- The impact of prey and predators on juvenile salmonids

USGS Publications Warehouse

Sauter, Sally T.; Schrock, Robin M.; Petersen, James H.; Maule, Alec G.

2004-01-01

The Bonneville Power Administration (BPA) has funded the Assessment of Smolt Condition project since 1987. During that time the project changed frequently to meet the information needs of fish managers by conducting studies throughout the Columbia River basin. Past research has examined the influence of smolt physiological development and health on migration rate; differences in development and migration rates of smolts of hatchery or wild origins; and the impacts of hatchery practices on smolt development. The Smolt Assessment Project will not continue beyond 2004, and here we report on the final study of the project in which we used bioenergetics modeling to investigate predation on juvenile salmonids by northern pikeminnow, smallmouth bass, and walleye in the lower Columbia River reservoirs.

Projected impact of urbanization on cardiovascular disease in China.

PubMed

Chan, Faye; Adamo, Susana; Coxson, Pamela; Goldman, Lee; Gu, Dongfeng; Zhao, Dong; Chen, Chung-Shiuan; He, Jiang; Mara, Valentina; Moran, Andrew

2012-10-01

The Coronary Heart Disease (CHD) Policy Model-China, a national scale cardiovascular disease computer simulation model, was used to project future impact of urbanization. Populations and cardiovascular disease incidence rates were stratified into four submodels: North-Urban, South-Urban, North-Rural, and South-Rural. 2010 was the base year, and high and low urbanization rate scenarios were used to project 2030 populations. Rural-to-urban migration, population growth, and aging were projected to more than double cardiovascular disease events in urban areas and increase events by 27.0-45.6% in rural areas. Urbanization is estimated to raise age-standardized coronary heart disease incidence by 73-81 per 100,000 and stroke incidence only slightly. Rural-to-urban migration will likely be a major demographic driver of the cardiovascular disease epidemic in China.

The Influence of Seal Properties on Pressure Buildup and Leakage of Carbon Dioxide from Sequestration Reservoirs (Invited)

NASA Astrophysics Data System (ADS)

Benson, S. M.; Chabora, E.

2009-12-01

The transport properties of seals, namely permeability, relative permeability, and capillary pressure control both migration of carbon dioxide and brine through the seal. Only recently has the the importance of brine migration emerged as key issue in the environmental performance of carbon dioxide sequestration projects. In this study we use numerical simulation to show that brine migration through the seal can be either advantageous or deleterious to the environmental performance of a carbon dioxide sequestration project. Brine migration through the seal can lower the pressure buildup in the storage reservoir, thereby reducing the risk of leakage or geomechanical stresses on the seal. On the other hand, if the seal is penetrated by a permeable fault it can lead to focused flow up a fault, which could lead to brine migration into drinking water aquifers. We also show that as the carbon dioxide plume grows, brine flow undergoes a complex evolution from upward flow to downward flows driven by countercurrent migration of carbon dioxide and brine in the seal and capillary pressure gradients at the base of the seal. Finally, we discuss desirable attributes seals, taking into account both carbon dioxide and brine migration through the seal. In particular, identifying seals that provide an effective capillary barrier to block the flow of carbon dioxide while allowing some brine migration through the seal can help to control pressure buildup and allow more efficient utilization of a sequestration reservoir. This could be particularly important in those settings that may be limited by the maximum allowable pressure buildup.

Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain.

PubMed

Nakamuta, Shinichi; Yang, Yu-Ting; Wang, Chia-Lin; Gallo, Nicholas B; Yu, Jia-Ray; Tai, Yilin; Van Aelst, Linda

2017-12-04

Throughout life, stem cells in the ventricular-subventricular zone generate neuroblasts that migrate via the rostral migratory stream (RMS) to the olfactory bulb, where they differentiate into local interneurons. Although progress has been made toward identifying extracellular factors that guide the migration of these cells, little is known about the intracellular mechanisms that govern the dynamic reshaping of the neuroblasts' morphology required for their migration along the RMS. In this study, we identify DOCK7, a member of the DOCK180-family, as a molecule essential for tangential neuroblast migration in the postnatal mouse forebrain. DOCK7 regulates the migration of these cells by controlling both leading process (LP) extension and somal translocation via distinct pathways. It controls LP stability/growth via a Rac-dependent pathway, likely by modulating microtubule networks while also regulating F-actin remodeling at the cell rear to promote somal translocation via a previously unrecognized myosin phosphatase-RhoA-interacting protein-dependent pathway. The coordinated action of both pathways is required to ensure efficient neuroblast migration along the RMS. © 2017 Nakamuta et al.

Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain

PubMed Central

Yang, Yu-Ting; Yu, Jia-Ray; Tai, Yilin

2017-01-01

Throughout life, stem cells in the ventricular–subventricular zone generate neuroblasts that migrate via the rostral migratory stream (RMS) to the olfactory bulb, where they differentiate into local interneurons. Although progress has been made toward identifying extracellular factors that guide the migration of these cells, little is known about the intracellular mechanisms that govern the dynamic reshaping of the neuroblasts’ morphology required for their migration along the RMS. In this study, we identify DOCK7, a member of the DOCK180-family, as a molecule essential for tangential neuroblast migration in the postnatal mouse forebrain. DOCK7 regulates the migration of these cells by controlling both leading process (LP) extension and somal translocation via distinct pathways. It controls LP stability/growth via a Rac-dependent pathway, likely by modulating microtubule networks while also regulating F-actin remodeling at the cell rear to promote somal translocation via a previously unrecognized myosin phosphatase–RhoA–interacting protein-dependent pathway. The coordinated action of both pathways is required to ensure efficient neuroblast migration along the RMS. PMID:29089377

Emerging U.S. Immigrant Geographies: Racial Wages and Migration Selectivity*

PubMed Central

Goodwin-White, Jamie

2014-01-01

Objective The maturing of the post-1965 children of immigrants and the recent emergence of immigrant settlement outside of traditional locations have implications for understanding immigrant economic incorporation. This analysis examines how changing immigrant geographies will affect the economic prospects of immigrants and a maturing second generation, and addresses sociological and economic perspectives on internal migration and immigrant progress. Methods Using the 2000 5 percent Public Use Microdata Files (PUMS), I employ endogenous switching regression models in analyzing the selectivity of internal migration and state residence patterns to the wages of immigrant, 1.5 generation, and U.S.-born workers. Results Nonwhite immigrant and 1.5-generation workers evade racial wage penalties through migration, but not through residing in emerging immigrant states. Conclusions Understanding the selectivity of internal migration to wages across racialized labor markets is important in assessing new immigrant geographies and prospects for the second generation. PMID:25364059

Opportunity and Challenges for Migrating Big Data Analytics in Cloud

NASA Astrophysics Data System (ADS)

Amitkumar Manekar, S.; Pradeepini, G., Dr.

2017-08-01

Big Data Analytics is a big word now days. As per demanding and more scalable process data generation capabilities, data acquisition and storage become a crucial issue. Cloud storage is a majorly usable platform; the technology will become crucial to executives handling data powered by analytics. Now a day’s trend towards “big data-as-a-service” is talked everywhere. On one hand, cloud-based big data analytics exactly tackle in progress issues of scale, speed, and cost. But researchers working to solve security and other real-time problem of big data migration on cloud based platform. This article specially focused on finding possible ways to migrate big data to cloud. Technology which support coherent data migration and possibility of doing big data analytics on cloud platform is demanding in natute for new era of growth. This article also gives information about available technology and techniques for migration of big data in cloud.

NOR1 promotes hepatocellular carcinoma cell proliferation and migration through modulating the Notch signaling pathway

DOE Office of Scientific and Technical Information (OSTI.GOV)

You, Kun; Sun, Peisheng; Yue, Zhongyi

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Previous studies have reported that the oxidored-nitro domain containing protein 1 (NOR1) is a novel tumor suppressor in several tumors. Recent evidence suggests that NOR1 is strongly expressed in HCC cells. However, its role and mechanism in HCC are unclear. In the current study, Western blot and qPCR detected strong NOR1 mRNA and protein expression in HepG2 and Hep3B cells. After transfection with NOR1 siRNA or pcDNA3.1-myc-his-NOR1, the proliferation and migration of HepG2 and Hep3B cells were analyzed in vitro. HepG2 or Hep3B cells overexpressing NOR1 showed anmore » increased proliferation and migration, whereas siRNA-mediated silencing of NOR1 showed the opposite effect. Furthermore, NOR1 activated the Notch signaling pathway, indicated by increased levels of Notch1, NICD, Hes1, and Hey1 in protein. Importantly, the Notch inhibitor DAPT downregulated Notch activation and further enhanced siNOR1-induced reduction of cell proliferation and migration in HepG2 and Hep3B cells, whereas DAPT reversed the effect of NOR1 overexpression on cell proliferation and migration. In conclusion, these results indicate that NOR1 may be involved in the progression of HCC and thus may be a potential target for the treatment of liver cancer. - Highlights: • NOR1 expression is up-regulated in HCC cells. • NOR1 promotes the proliferation and migration of HCC cells. • NOR1 promotes the progression of HCC cells by activating Notch pathway.« less

C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment.

PubMed

Urata, Satoko; Izumi, Kouji; Hiratsuka, Kaoru; Maolake, Aerken; Natsagdorj, Ariunbold; Shigehara, Kazuyoshi; Iwamoto, Hiroaki; Kadomoto, Suguru; Makino, Tomoyuki; Naito, Renato; Kadono, Yoshifumi; Lin, Wen-Jye; Wufuer, Guzailinuer; Narimoto, Kazutaka; Mizokami, Atsushi

2018-03-01

Chemokines and their receptors have key roles in cancer progression. The present study investigated chemokine activity in the prostate cancer bone metastasis microenvironment. Growth and migration of human prostate cancer cells were assayed in cocultures with bone stromal cells. The migration of LNCaP cells significantly increased when co-cultured with bone stromal cells isolated from prostate cancer bone metastases. Cytokine array analysis of conditioned medium from bone stromal cell cultures identified CCL5 as a concentration-dependent promoter of LNCaP cell migration. The migration of LNCaP cells was suppressed when C-C motif ligand 5 (CCL5) neutralizing antibody was added to cocultures with bone stromal cells. Knockdown of androgen receptor with small interfering RNA increased the migration of LNCaP cells compared with control cells, and CCL5 did not promote the migration of androgen receptor knockdown LNCaP. Elevated CCL5 secretion in bone stromal cells from metastatic lesions induced prostate cancer cell migration by a mechanism consistent with CCL5 activity upstream of androgen receptor signaling. © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Low junctional adhesion molecule A expression correlates with poor prognosis in gastric cancer.

PubMed

Huang, Jin-Yu; Xu, Ying-Ying; Sun, Zhe; Wang, Zhen-Ning; Zhu, Zhi; Song, Yong-Xi; Luo, Yang; Zhang, Xue; Xu, Hui-Mian

2014-12-01

The aberrant expression of junctional adhesion molecule A (JAM-A), which has a close correlation with the development, progression, metastasis, and prognosis of cancer, has been frequently reported. However, neither JAM-A expression nor its correlation with clinicopathologic variables and patient survival has been defined in gastric cancers. Moreover, little is known about the role of JAM-A in gastric cancer progression. We carried out the present study to investigate the prognostic value of JAM-A expression in gastric cancer patients. Furthermore, the biological roles of JAM-A in gastric cancer progression were also investigated. We determined JAM-A expression in 167 primary gastric cancer tissues and 94 matched adjacent non-tumor tissues by immunohistochemistry. Transwell migration assays and matrigel invasion assays were used to explore the role of JAM-A in gastric cancer cells migration and invasion. CCK-8 assays were used to examine the effect of JAM-A on the proliferation of gastric cancer cells. JAM-A was downregulated in gastric cancer tissues. Low JAM-A expression was significantly associated with tumor size, lymphatic vessel invasion, lymph node metastasis, and TNM stage. Low JAM-A expression was also significantly associated with poor disease-specific survival in gastric cancer patients. Multivariate analysis demonstrated low JAM-A expression as an independent factor predicting poor survival. In addition, JAM-A had the effect on inhibition of gastric cancer cells migration and invasion. However, JAM-A had no significant effects on proliferation of gastric cancer cells. Low JAM-A expression correlates with poor clinical outcome and promotes cell migration and invasion in gastric cancer. Copyright © 2014 Elsevier Inc. All rights reserved.

Exosomes-mediated transfer of long noncoding RNA ZFAS1 promotes gastric cancer progression.

PubMed

Pan, Lei; Liang, Wei; Fu, Min; Huang, Zhen-Hua; Li, Xia; Zhang, Wen; Zhang, Peng; Qian, Hui; Jiang, Peng-Cheng; Xu, Wen-Rong; Zhang, Xu

2017-06-01

ZFAS1 is a newly identified long noncoding RNA (lncRNA) that promotes tumor growth and metastasis. Exosomes mediate cellular communications in cancer by transmitting active molecules. The presence of ZFAS1 in the circulating exosomes and the roles of exosomal ZFAS1 in gastric cancer (GC) remains unknown. The aim of this study was to investigate the potential roles of exosomal ZFAS1 in GC. The expression of ZFAS1 was examined in the tumor tissues, serum samples, serum exosomes of GC patients and cell lines using qRT-PCR. The correlation between ZFAS1 expression and the clinicopathological characteristics was analyzed. The characteristics of exosomes were identified using transmission electron microscope (TEM), Nanoparticle Tracking Analysis (NTA), and western blot. The biological roles of ZFAS1 in GC cell growth and mobility were investigated using cell counting, cell colony formation, and transwell migration assay. The potential mechanism of ZFAS1 was demonstrated using flow cytometry, western blot, and qRT-PCR. ZFAS1 expression was elevated in GC cells, tumor tissues, serum and serum exosomes of GC patients. The increased ZFAS1 expression was significantly correlated with lymphatic metastasis and TNM stage. ZFAS1 knockdown inhibited the proliferation and migration of GC cells by suppressing cell cycle progression, inducing apoptosis, and inhibiting epithelial-mesenchymal transition (EMT). On the contrary, ZFAS1 overexpression promoted the proliferation and migration of GC cells. Moreover, ZFAS1 was present in exosomes and could be transmitted by exosomes to enhance GC cell proliferation and migration. ZFAS1 could be delivered by exosomes to promote GC progression, which suggests that ZFAS1 may serve as a potential diagnostic and prognostic biomarker for GC.

Investigations into the Early Life History of Naturally Produced Spring Chinook Salmon in the Grande Ronde Riiver Basin : Fish Research Project Oregon : Annual Progress Report 1 September 1995 to 1 August 1996.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jonasson, Brian C.; Carmichael, Richard W.; Keefe, MaryLouise

1997-09-01

Historically, the Grande Ronde River produced an abundance of salmonids including stocks of spring, summer and fall chinook salmon, sockeye salmon, coho salmon, and summer steelhead. During the past century, numerous factors have caused the reduction of salmon stocks such that only sustainable stocks of spring chinook salmon and summer steelhead remain. The sizes of spring chinook salmon populations in the Grande Ronde River basin also have been declining steadily and are substantially depressed from estimates of historic levels. In addition to a decline in population abundance, a reduction of spring chinook salmon spawning distribution is evident in the Grandemore » Ronde River basin. Numerous factors are thought to contribute to the decline of spring chinook salmon in the Snake River and its tributaries. These factors include passage problems and increased mortality of juvenile and adult migrants at mainstem Columbia and Snake river dams, overharvest, and habitat degradation associated with timber, agricultural, and land development practices. This study was designed to describe aspects of the life history strategies exhibited by spring chinook salmon in the Grande Ronde River basin. During the past year the focus was on rearing and migration patterns of juveniles in the upper Grande Ronde River and Catherine Creek. The study design included three objectives: (1) document the annual in-basin migration patterns for spring chinook salmon juveniles in the upper Grande Ronde River and Catherine Creek, including the abundance of migrants, migration timing and duration; (2) estimate and compare smolt survival indices to mainstem Columbia and Snake river dams for fall and spring migrating spring chinook salmon; and (3) determine summer and winter habitat utilization and preference of juvenile spring chinook salmon in the upper Grande Ronde River and Catherine Creek.« less

Rainfall Patterns and U.S. Migration from Rural Mexico

PubMed Central

Hunter, Lori M.; Murray, Sheena; Riosmena, Fernando

2014-01-01

In many rural regions of developing countries, natural resource dependency means changes in climate patterns hold tremendous potential to impact livelihoods. When environmentally-based livelihood options are constrained, migration can become an important adaptive strategy. Using data from the Mexican Migration Project, we model U.S. emigration from rural communities as related to community, household and climate factors. The results suggest that households subjected to recent drought conditions are far more likely to send a U.S. migrant, but only in communities with strong migration histories. In regions lacking such social networks, rainfall deficits actually reduce migration propensities, perhaps reflecting constraints in the ability to engage in migration as a coping strategy. Policy implications emphasize diversification of rural Mexican livelihoods in the face of contemporary climate change. PMID:25473143

Socioeconomic context and the association between marriage and Mexico-U.S. migration.

PubMed

Riosmena, Fernando

2009-06-01

In this paper, I analyze how the association between Mexico-U.S. migration and marriage varies across socioeconomic settings in origins. Using Mexican Migration Project data and employing bilevel survival analysis with controls for socioeconomic, migrant network, and marriage market characteristics and family size, I find that single people are most likely to migrate relative to those married in areas of recent industrialization, where the Mexican patriarchal system is weaker and economic opportunities for both men and women make post-marital migration less attractive. Marital status is not significant in agriculture-dependent areas, where the bargaining power of husbands might be higher relative to other settings; their age-profiles of earnings flatter; and remunerated female work scarcer, making migration attractive later in the life course.

Socioeconomic context and the association between marriage and Mexico–U.S. migration

PubMed Central

Riosmena, Fernando

2009-01-01

In this paper, I analyze how the association between Mexico–U.S. migration and marriage varies across socioeconomic settings in origins. Using Mexican Migration Project data and employing bilevel survival analysis with controls for socioeconomic, migrant network, and marriage market characteristics and family size, I find that single people are most likely to migrate relative to those married in areas of recent industrialization, where the Mexican patriarchal system is weaker and economic opportunities for both men and women make post-marital migration less attractive. Marital status is not significant in agriculture-dependent areas, where the bargaining power of husbands might be higher relative to other settings; their age-profiles of earnings flatter; and remunerated female work scarcer, making migration attractive later in the life course. PMID:19756216

Removal of small dams and its influence on physical habitat for salmonids in a Norwegian river

NASA Astrophysics Data System (ADS)

Fjeldstad, Hans-Petter; Barlaup, Bjørn; Stickler, Morten; Alfredsen, Knut; Gabrielsen, Sven-Erik

2010-05-01

While research and implementation of upstream migration solutions is extensive, and indeed often successful, full scale restoration projects and investigations of their influence on fish biology are rare in Norway. Acid deposition in Norwegian catchments peaked in the 1980's and resulted in both chronically and episodically acidified rivers and Salmonids in River Nidelva, one of the largest cathments in southern Norway, where extinct for decades. During this period hydropower development in the river paid limited attention to aquatic ecology. Weirs were constructed for esthetic purposes in the late 1970's and turned a 3 km stretch into a lake habitat, well suited for lake dwelling fish species, but unsuited for migration, spawning and juvenile habitat for salmonids. Since 2005, continuous liming to mitigate acidification has improved the water quality and a program for reintroduction of Atlantic salmon has been implemented. We used hydraulic modeling to plan the removal of two weirs on a bypass reach of the river. The 50 meters wide concrete weirs were blasted and removed in 2007, and ecological monitoring has been carried out in the river to assess the effect of weir removal. Topographic mapping, hydraulic measurements and modeling, in combination with biological surveys before and after the removal of the weirs, has proved to represent a powerful method for design of physical habitat adjustments and assessing their influence on fish biology. The model results also supported a rapid progress of planning and executing of the works. While telemetry studies before weir removal suggested that adult migration past the weirs was delayed with several weeks the fish can now pass the reach with minor obstacles. Spawning sites were discovered in the old bed substrate and were occupied already the first season after water velocities increased to suitable levels for spawning. Accordingly, the densities of Atlantic salmon juveniles have shown a marked increased after the conclusion of the project. Catches of pike and cyprinids on the reach is reduced, indicating that their habitat is no longer suitable, while salmon anglers have found new favorite spots in the restored pools and runs.

IL-17B activated mesenchymal stem cells enhance proliferation and migration of gastric cancer cells.

PubMed

Bie, Qingli; Zhang, Bin; Sun, Caixia; Ji, Xiaoyun; Barnie, Prince Amoah; Qi, Chen; Peng, Jingjing; Zhang, Danyi; Zheng, Dong; Su, Zhaoliang; Wang, Shengjun; Xu, Huaxi

2017-03-21

Mesenchymal stem cells are important cells in tumor microenvironment. We have previously demonstrated that IL-17B/IL-17RB signal promoted progression of gastric cancer. In this study, we further explored the effect of IL-17B on mesenchymal stem cells in tumor microenvironment and its impact on the tumor progression. The results showed that IL-17B induced the expression of stemness-related genes Nanog, Sox2, and Oct4 in mesenchymal stem cells and enhanced its tumor-promoting effect. The supernatant from cultured mesenchymal stem cells after treating with exogenous rIL-17B promoted the proliferation and migration of MGC-803, therefor suggesting that rIL-17B might promote mesenchymal stem cells to produce soluble factors. In addition, rIL-17B also activated the NF-κΒ, STAT3, β-catenin pathway in mesenchymal stem cells. Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells.

miR-132 suppresses the migration and invasion of lung cancer cells by blocking USP9X-induced epithelial-mesenchymal transition

PubMed Central

Guo, Huihui; Zhang, Xilin; Chen, Qiuqiang; Bao, Ying; Dong, Chaohui; Wang, Xiang

2018-01-01

miR-132, a microRNA, has been reported to be down-regulated in several human cancers and is related with tumor progression; however, its function in non-small cell lung cancer (NSCLC) progression remains unclear. This study aimed to investigate the putative role of miR-132 in the metastasis of NSCLC. We determined the function of miR-132 in the migration and invasion of a NSCLC cell line in vitro using a miR-132 inhibitor and mimic. Our results showed overexpression of miR-132 significantly inhibited the migration and invasion of NSCLC cells in vitro. We then identified USP9X as a potential target of miR-132, and demonstrated miR-132 could regulate the expression of USP9X at both the mRNA and protein level. miR-132 could directly bind to the 3’ untranslated region (3’-UTR) of USP9X. Inhibition of USP9X by its inhibitor WP1130 reduced the migration and invasion of NSCLC cells. Furthermore, USP9X inhibition also reversed the increased migration and invasion mediated by miR-132 inhibition. We found USP9X inhibition up-regulated expression of the epithelial-mesenchymal transition (EMT) marker E-cadherin, but down-regulated vimentin expression. A similar effect was seen with miR-132 overexpression, while the opposite effect occurred with miR-132 knockdown. USP9X inhibition reversed the miR-132 inhibitor-induced vimentin up-regulation and E-cadherin down-regulation. Taken together, these results indicate miR-132 prohibits the migration and invasion of NSCLC cells via targeting USP9X-induced EMT. Our data provides further evidence for the critical role of miR-132 and USP9X in regulating cell invasion and migration of NSCLC. PMID:29423007

miR-132 suppresses the migration and invasion of lung cancer cells by blocking USP9X-induced epithelial-mesenchymal transition.

PubMed

Guo, Huihui; Zhang, Xilin; Chen, Qiuqiang; Bao, Ying; Dong, Chaohui; Wang, Xiang

2018-01-01

miR-132, a microRNA, has been reported to be down-regulated in several human cancers and is related with tumor progression; however, its function in non-small cell lung cancer (NSCLC) progression remains unclear. This study aimed to investigate the putative role of miR-132 in the metastasis of NSCLC. We determined the function of miR-132 in the migration and invasion of a NSCLC cell line in vitro using a miR-132 inhibitor and mimic. Our results showed overexpression of miR-132 significantly inhibited the migration and invasion of NSCLC cells in vitro . We then identified USP9X as a potential target of miR-132, and demonstrated miR-132 could regulate the expression of USP9X at both the mRNA and protein level. miR-132 could directly bind to the 3' untranslated region (3'-UTR) of USP9X. Inhibition of USP9X by its inhibitor WP1130 reduced the migration and invasion of NSCLC cells. Furthermore, USP9X inhibition also reversed the increased migration and invasion mediated by miR-132 inhibition. We found USP9X inhibition up-regulated expression of the epithelial-mesenchymal transition (EMT) marker E-cadherin, but down-regulated vimentin expression. A similar effect was seen with miR-132 overexpression, while the opposite effect occurred with miR-132 knockdown. USP9X inhibition reversed the miR-132 inhibitor-induced vimentin up-regulation and E-cadherin down-regulation. Taken together, these results indicate miR-132 prohibits the migration and invasion of NSCLC cells via targeting USP9X-induced EMT. Our data provides further evidence for the critical role of miR-132 and USP9X in regulating cell invasion and migration of NSCLC.

Focus: Asian migration to Canada.

PubMed

Kobayashi, A

1988-01-01

This collection of 5 short essays on Asian migration to Canada focuses on the relationships between individual migrants and their social contexts, both Asian and Canadian. Papers by Anderson and Kobayashi adopt research perspectives of outsider and insider, respectively. Vibert provides a historical overview against which the substantive issues introduced in the other 3 papers can be understood, and he illustrates the links between circumstances of migration and the larger issues by which the course of Canadian social progress has been steered. Mercer provides an introduction to issues that dominate the agenda of contemporary research, to show that Canadian communities of Asian heritage continue to grow in size, diversity, and complexity, as they become more established on the Canadian landscape. This collection is as much about the geography of racism as it is about migration.

Distinctive and selective route of PI3K/PKCα-PKCδ/RhoA-Rac1 signaling in osteoclastic cell migration.

PubMed

Kim, Jin-Man; Kim, Mi Yeong; Lee, Kyunghee; Jeong, Daewon

2016-12-05

Cell migration during specialized stages of osteoclast precursors, mononuclear preosteoclasts, and multinucleated mature osteoclasts remain uncertain. M-CSF- and osteopontin-induced osteoclastic cell migration was inhibited by function-blocking monoclonal antibodies specific to the integrin αv and β3 subunits, suggesting that integrin αvβ3 mediates migratory signaling induced by M-CSF and osteopontin. M-CSF and osteopontin stimulation was shown to regulate two branched signaling processes, PI3K/PKCα/RhoA axis and PI3K/PKCδ/Rac1 axis. Interestingly, inactivation of RhoA or Rac1 blocked preosteoclast and mature osteoclast migration but not osteoclast precursor migration in a transwell-based cell migration assay. Moreover, the inhibitory effect on preosteoclast and mature osteoclast migration induced by Rac1 inactivation was more effective than that by RhoA inactivation. Collectively, our findings suggest that osteoclast precursor migration depends on PI3K/PKCα-PKCδ signaling mediated via integrin αvβ3 bypassing RhoA and Rac1, whereas preosteoclast and mature osteoclast migration relies on PI3K/PKCα-PKCδ/RhoA-Rac1 axis signaling mediated via integrin αvβ3 with increased dependency on PKCδ/Rac1 signaling route as differentiation progresses. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

40 CFR 35.1650-6 - Reports.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Reports. (a) States with Phase 1 projects shall submit semi-annual progress reports (original and one copy... in the next six months. (b) Phase 2. States with Phase 2 projects shall submit progress reports... Phase 2 project progress reports shall be determined by the size and complexity of the project, and...

40 CFR 35.1650-6 - Reports.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Reports. (a) States with Phase 1 projects shall submit semi-annual progress reports (original and one copy... in the next six months. (b) Phase 2. States with Phase 2 projects shall submit progress reports... Phase 2 project progress reports shall be determined by the size and complexity of the project, and...

CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zheng, Jiajia; Zhu, Xi; Zhang, Jie, E-mail: zhangjiebjmu@163.com

2014-03-28

Highlights: • We first demonstrated CXCL5 is highly expressed in human bladder tumor tissues and cells. • CXCL5 knockdown inhibits proliferation, migration and promotes apoptosis in T24 cells. • CXCL5 knockdown inhibits Snail, PI3K-AKT and ERK1/2 signaling pathways in T24 cells. • CXCL5 is critical for bladder tumor growth and progression. - Abstract: CXCL5 (epithelial neutrophil activating peptide-78) which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. To investigate the role of CXCL5 in bladder cancer progression, we examined the CXCL5 expression in bladder cancer tissues by real-time PCRmore » and Western blot, additionally, we used shRNA-mediated silencing to generate stable CXCL5 silenced bladder cancer T24 cells and defined its biological functions. Our results demonstrated that mRNA and protein of CXCL5 is increased in human bladder tumor tissues and cell lines, down-regulation of CXCL5 in T24 cells resulted in significantly decreased cell proliferation, migration and increased cell apoptosis in vitro through Snail, PI3K-AKT and ERK1/2 signaling pathways. These data suggest that CXCL5 is critical for bladder tumor growth and progression, it may represent a potential application in cancer diagnosis and therapy.« less

Tenascin-C, proliferation and subendothelial fibronectin in progressive pulmonary vascular disease.

PubMed Central

Jones, P. L.; Cowan, K. N.; Rabinovitch, M.

1997-01-01

Progressive pulmonary hypertension is characterized by smooth muscle cell proliferation and migration leading to occlusive arterial lesions. Previously, using cultured smooth muscle cells, we demonstrated that epidermal growth factor (EGF)-dependent proliferation and migration are dependent on tenascin-C (Tn) and cellular fibronectin (Fn), respectively. In this study we applied immunohistochemistry to lung biopsy tissue from patients with congenital heart defects and pulmonary hypertension to determine how the distribution and intensity of Tn, EGF, proliferating cell nuclear antigen (PCNA), and Fn expression related to arterial abnormalities. With mildly increased wall thickness, minimal Tn, PCNA, and EGF was evident. With progressive hypertrophy, moderately intense foci of Tn were apparent in the adventitia, periendothelium, and occasionally the media but not consistently co-distributing with EGF and PCNA. With obstructive lesions, intense neointimal Tn expression co-localized with EGF and PCNA. Fn accumulation in the periendothelium increased with medial hypertrophy and became more widespread in a diffuse pattern with neointimal formation. The neointima was predominantly composed of alpha-smooth-muscle-actin-positive cells, occasional inflammatory cells with no evidence of apoptosis. These studies are consistent with Tn modulating EGF-dependent neointimal smooth muscle cell proliferation and Fn providing a gradient for smooth muscle cell migration from media to neointima. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:9094991

The role of migration in the development of depressive symptoms among Latino immigrant parents in the USA.

PubMed

Ornelas, India J; Perreira, Krista M

2011-10-01

Nearly one out of every four children in the US is a child of immigrants. Yet few studies have assessed how factors at various stages of migration contribute to the development of health problems in immigrant populations. Most focus only on post-migration factors influencing health. Using data from the Latino Adolescent Migration, Health, and Adaptation Project, this study assessed the extent to which pre-migration (e.g., major life events, high poverty), migration (e.g., unsafe and stressful migration experiences), post-migration (e.g., discrimination, neighborhood factors, family reunification, linguistic isolation), and social support factors contributed to depressive symptoms among a sample of Latino immigrant parents with children ages 12-18. Results indicated that high poverty levels prior to migration, stressful experiences during migration, as well as racial problems in the neighborhood and racial/ethnic discrimination upon settlement in the US most strongly contribute to the development of depressive symptoms among Latino immigrant parents. Family reunification, social support, and familism reduce the likelihood of depressive symptoms. Copyright © 2011 Elsevier Ltd. All rights reserved.

The Role of Migration in the Development of Depressive Symptoms among Latino Immigrant Parents in the USA

PubMed Central

Ornelas, India J.; Perreira, Krista M.

2011-01-01

Nearly one out of every four children in the US is a child of immigrants. Yet few studies have assessed how factors at various stages of migration contribute to the development of health problems in immigrant populations. Most focus only on post-migration factors influencing health. Using data from the Latino Adolescent Migration, Health, and Adaptation Project, this study assessed the extent to which pre-migration (e.g., major life events, high poverty), migration (e.g., unsafe and stressful migration experiences), post-migration (e.g., discrimination, neighborhood factors, family reunification, linguistic isolation), and social support factors contributed to depressive symptoms among a sample of Latino immigrant parents with children ages 12-18. Results indicated that high poverty levels prior to migration, stressful experiences during migration, as well as racial problems in the neighborhood and racial/ethnic discrimination upon settlement in the US most strongly contribute to the development of depressive symptoms among Latino immigrant parents. Family reunification, social support, and familism reduce the likelihood of depressive symptoms. PMID:21908089

Podoplanin increases migration and angiogenesis in malignant glioma

PubMed Central

Grau, Stefan J; Trillsch, Fabian; Tonn, Joerg-Christian; Goldbrunner, Roland H; Noessner, Elfriede; Nelson, Peter J; von Luettichau, Irene

2015-01-01

Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373Phigh/U87Phigh). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87Phigh, where a VEGFR3 expression was induced. U373Phigh showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from Phigh glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression. PMID:26339454

Post-project geomorphic assessment of a large process-based river restoration project

USGS Publications Warehouse

Erwin, Susannah O.; Schmidt, John C.; Allred, Tyler M.

2016-01-01

This study describes channel changes following completion of the Provo River Restoration Project (PRRP), the largest stream restoration project in Utah and one of the largest projects in the United States in which a gravel-bed river was fully reconstructed. We summarize project objectives and the design process, and we analyze monitoring data collected during the first 7 years after project completion. Post-project channel adjustment during the study period included two phases: (i) an initial phase of rapid, but small-scale, adjustment during the first years after stream flow was introduced to the newly constructed channel and (ii) a subsequent period of more gradual topographic adjustment and channel migration. Analysis of aerial imagery and ground-survey data demonstrate that the channel has been more dynamic in the downstream 4 km where a local source contributes a significant annual supply of bed material. Here, the channel migrates and exhibits channel adjustments that are more consistent with project objectives. The upstream 12 km of the PRRP are sediment starved, the channel has been laterally stable, and this condition may not be consistent with large-scale project objectives.

The development of the nursing profession in a globalised context: A qualitative case study in Kerala, India.

PubMed

Timmons, Stephen; Evans, Catrin; Nair, Sreelekha

2016-10-01

In the paper, we are looking at the relationship between globalisation and the professional project, using nursing in Kerala as an exemplar. Our focus is on the intersection of the professional project, gender and globalisation processes. Included in our analysis are the ways in which gender affects the professional project in the global south, and the development of a professional project which it is closely tied to global markets and global migration, revealing the political-economic, historical, and cultural factors that influence the shape and consequences of nurse migration. The phenomenon that enabled our analysis, by showing these forces at work in a particular time and place, was an outbreak of strikes by nurses working in private hospitals in Kerala in 2011-2012. Copyright © 2016 Elsevier Ltd. All rights reserved.

Next Generation Attenuation Relationships for the Eastern United States (NGA-East)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mahin, Stephen; Bozorgnia, Yousef

2016-04-11

This is a progress report to DOE for project Next Generation Attenuation for Central & Eastern US (NGA-East).This progress report consists of numerous monthly progress segments starting June 1, 2010 until December 31, 2015. Please note: the December 2015 progress report was issued in January 2016 due to the final university financial reporting at the end of this project. For each month, there is a technical progress list, and an update on the financial progress of the project. As you know, this project is jointly funded by the DOE, US Nuclear Regulatory Commission (NRC) and Electric Power Research Institute (EPRI).more » Thus, each segment includes financial progress for these three funding agencies.« less

Towards an optimal adaptation of exposure to NOAA assessment methodology in Multi-Source Industrial Scenarios (MSIS): the challenges and the decision-making process

NASA Astrophysics Data System (ADS)

López de Ipiña, JM; Vaquero, C.; Gutierrez-Cañas, C.

2017-06-01

It is expected a progressive increase of the industrial processes that manufacture of intermediate (iNEPs) and end products incorporating ENMs (eNEPs) to bring about improved properties. Therefore, the assessment of occupational exposure to airborne NOAA will migrate, from the simple and well-controlled exposure scenarios in research laboratories and ENMs production plants using innovative production technologies, to much more complex exposure scenarios located around processes of manufacture of eNEPs that, in many cases, will be modified conventional production processes. Here will be discussed some of the typical challenging situations in the process of risk assessment of inhalation exposure to NOAA in Multi-Source Industrial Scenarios (MSIS), from the basis of the lessons learned when confronted to those scenarios in the frame of some European and Spanish research projects.

The role of organic complexants and microparticulates in the facilitated transport of radionuclides

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schilk, A.J.; Robertson, D.E.; Abel, K.H.

1996-12-01

This progress report describes the results of ongoing radiological and geochemical investigations of the mechanisms of radionuclide transport in groundwater at two low-level waste (LLW) disposal sites within the waste management area of the Chalk River Laboratories (CRL), Ontario, Canada. These sites, the Chemical Pit liquid disposal facility and the Waste Management Area C solid LLW disposal site, have provided valuable 30- to 40-year-old field locations for characterizing the migration of radionuclides and evaluating a number of recent site performance objectives for LLW disposal facilities. This information will aid the NRC and other federal, state, and local regulators, as wellmore » as LLW disposal site developers and waste generators, in maximizing the effectiveness of existing or projected LLW disposal facilities for isolating radionuclides from the general public and thereby improving the health and safety aspects of LLW disposal.« less

Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.

PubMed

Vitriol, Eric A; Zheng, James Q

2012-03-22

Growth cones, found at the tip of axonal projections, are the sensory and motile organelles of developing neurons that enable axon pathfinding and target recognition for precise wiring of the neural circuitry. To date, many families of conserved guidance molecules and their corresponding receptors have been identified that work in space and time to ensure billions of axons to reach their targets. Research in the past two decades has also gained significant insight into the ways in which growth cones translate extracellular signals into directional migration. This review aims to examine new progress toward understanding the cellular mechanisms underlying directional motility of the growth cone and to discuss questions that remain to be addressed. Specifically, we will focus on the cellular ensemble of cytoskeleton, adhesion, and membrane and examine how the intricate interplay between these processes orchestrates the directed movement of growth cones. Copyright © 2012 Elsevier Inc. All rights reserved.

[Mobility of physicians in Europe: health policies and health care provision].

PubMed

Jourdain, Alain; Pham, Tam

2017-03-06

Objective: To define the place of geographical mobility of physicians in medical demography policies in EU countries. Methods: Review of international migration assumptions in national projection models of numbers of physicians by broad categories of social protection systems in the EU. Results: Some countries fail to achieve medium-term projections of the number of physicians and those that do adopt the assumption of net migration, assume that they converge to zero. Migration is not considered to be a solution to the expected shortage of physicians, but rather a problem to be solved. Discussion: Three approaches to labour mobility are discussed: liberal, normative and ethical. The last approach appears to be the most popular by combining preservation of national interests with the World Health Organisation Global Code of Practice on the International Recruitment of Health Personnel.

Orai1 and STIM1 are critical for cell migration and proliferation of clear cell renal cell carcinoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Ji-Hee; Lkhagvadorj, Sayamaa; Lee, Mi-Ra

2014-05-23

Highlights: • Orai1 channel is highly expressed in clear cell renal cell carcinoma (ccRCC) tissues. • Orai1 and STIM1 constitute a native store-operated Ca{sup 2+} entry in ccRCC cells. • Orai1 and STIM1 promote cell migration and proliferation of ccRCC cells. - Abstract: The intracellular Ca{sup 2+} regulation has been implicated in tumorigenesis and tumor progression. Notably, store-operated Ca{sup 2+} entry (SOCE) is a major Ca{sup 2+} entry mechanism in non-excitable cells, being involved in cell proliferation and migration in several types of cancer. However, the expression and biological role of SOCE have not been investigated in clear cell renalmore » cell carcinoma (ccRCC). Here, we demonstrate that Orai1 and STIM1, not Orai3, are crucial components of SOCE in the progression of ccRCC. The expression levels of Orai1 in tumor tissues were significantly higher than those in the adjacent normal parenchymal tissues. In addition, native SOCE was blunted by inhibiting SOCE or by silencing Orai1 and STIM1. Pharmacological blockade or knockdown of Orai1 or STIM1 also significantly inhibited RCC cell migration and proliferative capability. Taken together, Orai1 is highly expressed in ccRCC tissues illuminating that Orai1-mediated SOCE may play an important role in ccRCC development. Indeed, Orai1 and STIM1 constitute a native SOCE pathway in ccRCC by promoting cell proliferation and migration.« less

MITSI project : final local evaluation report

DOT National Transportation Integrated Search

2003-01-01

The mission statement for the MITSI project was facilitating National Standards Compliance migration for NaviGAtor, conducting National Architecture mapping for MARTA and E911, and evaluating CORBA as a methodology for exchanging data. This involved ...

Cell migration in microengineered tumor environments.

PubMed

Um, Eujin; Oh, Jung Min; Granick, Steve; Cho, Yoon-Kyoung

2017-12-05

Recent advances in microengineered cell migration platforms are discussed critically with a focus on how cell migration is influenced by engineered tumor microenvironments, the medical relevance being to understand how tumor microenvironments may promote or suppress the progression of cancer. We first introduce key findings in cancer cell migration under the influence of the physical environment, which is systematically controlled by microengineering technology, followed by multi-cues of physico-chemical factors, which represent the complexity of the tumor environment. Recognizing that cancer cells constantly communicate not only with each other but also with tumor-associated cells such as vascular, fibroblast, and immune cells, and also with non-cellular components, it follows that cell motility in tumor microenvironments, especially metastasis via the invasion of cancer cells into the extracellular matrix and other tissues, is closely related to the malignancy of cancer-related mortality. Medical relevance of forefront research realized in microfabricated devices, such as single cell sorting based on the analysis of cell migration behavior, may assist personalized theragnostics based on the cell migration phenotype. Furthermore, we urge development of theory and numerical understanding of single or collective cell migration in microengineered platforms to gain new insights in cancer metastasis and in therapeutic strategies.

How Tissue Mechanical Properties Affect Enteric Neural Crest Cell Migration

NASA Astrophysics Data System (ADS)

Chevalier, N. R.; Gazguez, E.; Bidault, L.; Guilbert, T.; Vias, C.; Vian, E.; Watanabe, Y.; Muller, L.; Germain, S.; Bondurand, N.; Dufour, S.; Fleury, V.

2016-02-01

Neural crest cells (NCCs) are a population of multipotent cells that migrate extensively during vertebrate development. Alterations to neural crest ontogenesis cause several diseases, including cancers and congenital defects, such as Hirschprung disease, which results from incomplete colonization of the colon by enteric NCCs (ENCCs). We investigated the influence of the stiffness and structure of the environment on ENCC migration in vitro and during colonization of the gastrointestinal tract in chicken and mouse embryos. We showed using tensile stretching and atomic force microscopy (AFM) that the mesenchyme of the gut was initially soft but gradually stiffened during the period of ENCC colonization. Second-harmonic generation (SHG) microscopy revealed that this stiffening was associated with a gradual organization and enrichment of collagen fibers in the developing gut. Ex-vivo 2D cell migration assays showed that ENCCs migrated on substrates with very low levels of stiffness. In 3D collagen gels, the speed of the ENCC migratory front decreased with increasing gel stiffness, whereas no correlation was found between porosity and ENCC migration behavior. Metalloprotease inhibition experiments showed that ENCCs actively degraded collagen in order to progress. These results shed light on the role of the mechanical properties of tissues in ENCC migration during development.

Leupaxin stimulates adhesion and migration of prostate cancer cells through modulation of the phosphorylation status of the actin-binding protein caldesmon

PubMed Central

Schmidt, Thomas; Bremmer, Felix; Burfeind, Peter; Kaulfuß, Silke

2015-01-01

The focal adhesion protein leupaxin (LPXN) is overexpressed in a subset of prostate cancers (PCa) and is involved in the progression of PCa. In the present study, we analyzed the LPXN-mediated adhesive and cytoskeletal changes during PCa progression. We identified an interaction between the actin-binding protein caldesmon (CaD) and LPXN and this interaction is increased during PCa cell migration. Furthermore, knockdown of LPXN did not affect CaD expression but reduced CaD phosphorylation. This is known to destabilize the affinity of CaD to F-actin, leading to dynamic cell structures that enable cell motility. Thus, downregulation of CaD increased migration and invasion of PCa cells. To identify the kinase responsible for the LPXN-mediated phosphorylation of CaD, we used data from an antibody array, which showed decreased expression of TGF-beta-activated kinase 1 (TAK1) after LPXN knockdown in PC-3 PCa cells. Subsequent analyses of the downstream kinases revealed the extracellular signal-regulated kinase (ERK) as an interaction partner of LPXN that facilitates CaD phosphorylation during LPXN-mediated PCa cell migration. In conclusion, we demonstrate that LPXN directly influences cytoskeletal dynamics via interaction with the actin-binding protein CaD and regulates CaD phosphorylation by recruiting ERK to highly dynamic structures within PCa cells. PMID:26079947

Emplacement of the Kodiak batholith and slab-window migration

USGS Publications Warehouse

Farris, David W.; Haeussler, Peter J.; Friedman, Richard; Paterson, Scott R.; Saltus, R.W.; Ayuso, Robert A.

2006-01-01

The Kodiak batholith is one of the largest, most elongate intrusive bodies in the forearc Sanak-Baranof plutonic belt located in southern Alaska. This belt is interpreted to have formed during the subduction of an oceanic spreading center and the associated migration of a slab window. Individual plutons of the Kodiak batholith track the location and evolution of the underlying slab window. Six U/Pb zircon ages from the axis of the batholith exhibit a northeastward-decreasing age progression of 59.2 ± 0.2 Ma at the southwest end to 58.4 ± 0.2 Ma at the northeast tip. The trench-parallel rate of age progression is within error of the average slab-window migration rate for the entire Sanak-Baranof belt (~19 cm/yr). Structural relationships, U/Pb ages, and a model of new gravity data indicate that magma from the Kodiak batholith ascended 5-10 km as a northeastward-younging series of 1-8-km-diameter viscoelastic diapirs. Individual plutons ascended by multiple emplacement mechanisms including downward flow, collapse of wall rock, stoping, and diking. Stokes flow xenolith calculations suggest ascent rates of 5-100 m/yr and an effective magmatic viscosity of 107-108 Pa s. Pre-existing structural or lithologic heterogeneities did not dominantly control the location of the main batholith. Instead, its location was determined by migration of the slab window at depth. 

Projecting population change in the interior Columbia River Basin.

Treesearch

Stephen F. McCool; Richard W. Haynes

1996-01-01

Management of ecosystems requires projecting the human population for a biologically significant timeframe, because the impacts of potential alternative ecosystem management strategies will differ depending on the size, location, and expectations of the human population. Increases since 1990 in the net migration rates are changing the expectations for projections of...

Polarised Clathrin-Mediated Endocytosis of EGFR During Chemotactic Invasion

PubMed Central

Mutch, Laura Jane; Howden, Jake Davey; Jenner, Emma Poppy Louise; Poulter, Natalie Sarah; Rappoport, Joshua Zachary

2014-01-01

Directed cell migration is critical for numerous physiological processes including development and wound healing. However chemotaxis is also exploited during cancer progression. Recent reports have suggested links between vesicle trafficking pathways and directed cell migration. Very little is known about the potential roles of endocytosis pathways during metastasis. Therefore we performed a series of studies employing a previously characterised model for chemotactic invasion of cancer cells to assess specific hypotheses potentially linking endocytosis to directed cell migration. Our results demonstrate that clathrin-mediated endocytosis is indispensable for epidermal growth factor (EGF) directed chemotactic invasion of MDA-MB-231 cells. Conversely, caveolar endocytosis is not required in this mode of migration. We further found that chemoattractant receptor (EGFR) trafficking occurs by clathrin-mediated endocytosis and is polarised towards the front of migrating cells. However, we found no role for clathrin-mediated endocytosis in focal adhesion disassembly in this migration model. Thus, this study has characterised the role of endocytosis during chemotactic invasion and has identified functions mechanistically linking clathrin-mediated endocytosis to directed cell motility. PMID:24921075

Tre1 GPCR initiates germ cell transepithelial migration by regulating Drosophila melanogaster E-cadherin

PubMed Central

Kunwar, Prabhat S.; Sano, Hiroko; Renault, Andrew D.; Barbosa, Vitor; Fuse, Naoyuki; Lehmann, Ruth

2008-01-01

Despite significant progress in identifying the guidance pathways that control cell migration, how a cell starts to move within an intact organism, acquires motility, and loses contact with its neighbors is poorly understood. We show that activation of the G protein–coupled receptor (GPCR) trapped in endoderm 1 (Tre1) directs the redistribution of the G protein Gβ as well as adherens junction proteins and Rho guanosine triphosphatase from the cell periphery to the lagging tail of germ cells at the onset of Drosophila melanogaster germ cell migration. Subsequently, Tre1 activity triggers germ cell dispersal and orients them toward the midgut for directed transepithelial migration. A transition toward invasive migration is also a prerequisite for metastasis formation, which often correlates with down-regulation of adhesion proteins. We show that uniform down-regulation of E-cadherin causes germ cell dispersal but is not sufficient for transepithelial migration in the absence of Tre1. Our findings therefore suggest a new mechanism for GPCR function that links cell polarity, modulation of cell adhesion, and invasion. PMID:18824569

Viability of long range dragonfly migration across the Indian Ocean: An energetics perspective

NASA Astrophysics Data System (ADS)

Saha, Sandeep; Nirwal, Satvik

2016-11-01

Recently Pantala flavescens (dragonflies) have been reported to migrate in millions from India to Eastern Africa on a multigenerational migratory circuit of length 14000-18000 kms. We attempt to understand the ability of dragonflies to perform long range migration by examining the energetics using computer simulations. In absence of a theory for long range insect migrations, we resort to the extensive literature on long range bird migration from the energetics perspective. The flight energetics depends upon instantaneous power and velocity. The mechanical flight power is computed from the power curve which is then converted to mass depletion using Brequet's equation. However, the mechanical flight power itself depends upon the instantaneous velocity which can vary depending upon the current mass. In order to predict the range in our simulations, we assume that the insect progressively tries to achieve the maximum range velocity. The results indicate that the migration range is approximately 1260 kms in 70 hours based on the true airspeed. However, our analysis is restricted by the lack of data and certain caveats in drag prediction and basal metabolism rate.

Claudin-1 promotes TNF-α-induced epithelial-mesenchymal transition and migration in colorectal adenocarcinoma cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhat, Ajaz A.; Ahmad, Rizwan; Uppada, SrijayaPrakash B.

Epithelial-mesenchymal transition (EMT) is an important mechanism in cancer progression and malignancy including colorectal cancer (CRC). Importantly, inflammatory mediators are critical constituents of the local tumor environment and an intimate link between CRC progression and inflammation is now validated. We and others have reported key role of the deregulated claudin-1 expression in colon carcinogenesis including colitis-associated colon cancer (CAC). However, the causal association between claudin-1 expression and inflammation-induced colon cancer progression remains unclear. Here we demonstrate, TNF-α, a pro-inflammatory cytokine, regulates claudin-1 to modulate epithelial to mesenchymal transition (EMT) and migration in colon adenocarcinoma cells. Importantly, colon cancer cells culturedmore » in the presence of TNF-α (10 ng/ml), demonstrated a sharp decrease in E-cadherin expression and an increase in vimentin expression (versus control cells). Interestingly, TNF-α treatment also upregulated (and delocalized) claudin-1 expression in a time-dependent manner accompanied by increase in proliferation and wound healing. Furthermore, similar to our previous observation that claudin-1 overexpression in CRC cells induces ERK1/2 and Src- activation, signaling associated with colon cancer cell survival and transformation, TNF-α-treatment induced upregulation of phospho-ERK1/2 and -Src expression. The shRNA-mediated inhibition of claudin-1 expression largely abrogated the TNF-α-induced changes in EMT, proliferation, migration, p-Erk and p-Src expression. Taken together, our data demonstrate TNF-α mediated regulation of claudin-1 and tumorigenic abilities of colon cancer cells and highlights a key role of deregulated claudin-1 expression in inflammation-induced colorectal cancer growth and progression, through the regulation of the ERK and Src-signaling.« less

Preserving the Whole: A Two-Track Approach to Rescuing Social Science Data and Metadata.

ERIC Educational Resources Information Center

Green, Ann; Dionne, JoAnn; Dennis, Martin

Focusing on the experience of the Yale University (Connecticut) social science data preservation project, this document presents a case study of migration as a preservation strategy, exploring options for migrating data stored in a technically obsolete format and their associated documentation stored on paper. The first section provides background…

Replacement Migration: Is It a Solution to Declining and Ageing Populations?

ERIC Educational Resources Information Center

United Nations, New York, NY. Dept. of Economic and Social Affairs.

The United Nations (UN) Population Division monitors fertility, mortality, and migration trends for all countries as a basis for producing the official UN population estimates and projections. Among recent demographic trends, two are prominent: (1) population decline and (2) population aging. Focusing on these two critical trends, a study…

TRPV2 mediates adrenomedullin stimulation of prostate and urothelial cancer cell adhesion, migration and invasion.

PubMed

Oulidi, Agathe; Bokhobza, Alexandre; Gkika, Dimitra; Vanden Abeele, Fabien; Lehen'kyi, V'yacheslav; Ouafik, L'houcine; Mauroy, Brigitte; Prevarskaya, Natalia

2013-01-01

Adrenomedullin (AM) is a 52-amino acid peptide initially isolated from human pheochromocytoma. AM is expressed in a variety of malignant tissues and cancer cell lines and was shown to be a mitogenic factor capable of stimulating growth of several cancer cell types. In addition, AM is a survival factor for certain cancer cells. Some data suggest that AM might be involved in the progression cancer metastasis via angiogenesis and cell migration and invasion control. The Transient Receptor Potential channel TRPV2 is known to promote in prostate cancer cell migration and invasive phenotype and is correlated with the stage and grade of bladder cancer. In this work we show that AM induces prostate and urothelial cancer cell migration and invasion through TRPV2 translocation to plasma membrane and the subsequent increase in resting calcium level.

Temperature-influenced energetics model for migrating waterfowl

USGS Publications Warehouse

Aagaard, Kevin; Thogmartin, Wayne E.; Lonsdorg, Eric V.

2018-01-01

Climate and weather affect avian migration by influencing when and where birds fly, the energy costs and risks of flight, and the ability to sense cues necessary for proper navigation. We review the literature of the physiology of avian migration and the influence of climate, specifically temperature, on avian migration dynamics. We use waterfowl as a model guild because of the ready availability of empirical physiological data and their enormous economic value, but our discussion and expectations are broadly generalizable to migratory birds in general. We detail potential consequences of an increasingly warm climate on avian migration, including the possibility of the cessation of migration by some populations and species. Our intent is to lay the groundwork for including temperature effects on energetic gains and losses of migratory birds with the expected consequences of increasing temperatures into a predictive modeling framework. To this end, we provide a simulation of migration progression exclusively focused on the influence of temperature on the physiological determinants of migration. This simulation produced comparable results to empirically derived and observed values for different migratory factors (e.g., body fat content, flight range, departure date). By merging knowledge from the arenas of avian physiology and migratory theory we have identified a clear need for research and have developed hypotheses for a path forward.

Controls on drainage divide migration in the northern Sierras Pampeanas assessed through morphometric indicators

NASA Astrophysics Data System (ADS)

Seagren, E. G.; Schoenbohm, L. M.

2017-12-01

Drainage reorganization, primarily through progressive divide migration leading to discrete stream captures, is increasingly recognized as a common phenomenon during mountain-building events. This drainage rearrangement reflects complex interactions between tectonics, climate, and lithology, and can fundamentally change erosion and sedimentation patterns; therefore, determining the spatial extent and potential controls of divide migration is vital to understanding the topographic evolution of orogenic landscapes. Both geomorphic and morphometric evidence can be used to identify such drainage reorganization. The northern Sierras Pampeanas is an ideal location in which to study divide migration as limited glaciation and low out-of-channel erosion rates preserve evidence of reorganization. Additionally, several ranges in the region, such as Sierra de las Planchadas, exhibit geomorphic evidence of drainage rearrangement, including wind gaps and hairpin turns. Using ArcGIS, LSDTopoTools, and TopoToolbox, we conducted a systematic analysis of the spatial distribution of three morphometric indicators of divide migration: χ, Mx, and local headwater relief. Local `hotspots' undergoing drainage divide migration were identified using spatial autocorrelation and clustering methods - Gi* and Moran's I. Using spatial regression analysis, we assessed the potential controls of lithology, modern TRMM precipitation rates, and tectonics over divide migration. Preliminary results suggest broad westward migration of main drainage divides, following both the orographic precipitation gradient and regional slope.

From Confrontation to Cooperation: 8th International Seminar on Nuclear War

NASA Astrophysics Data System (ADS)

Zichichi, A.; Dardo, M.

1992-09-01

The Table of Contents for the full book PDF is as follows: * OPENING SESSION * A. Zichichi: Opening Statements * R. Nicolosi: Opening Statements * MESSAGES * CONTRIBUTIONS * "The Contribution of the Erice Seminars in East-West-North-South Scientific Relations" * 1. LASER TECHNOLOGY * "Progress in laser technology" * "Progress in laboratory high gain ICF: prospects for the future" * "Applications of laser in metallurgy" * "Laser tissue interactions in medicine and surgery" * "Laser fusion" * "Compact X-ray lasers in the laboratory" * "Alternative method for inertial confinement" * "Laser technology in China" * 2. NUCLEAR AND CHEMICAL SAFETY * "Reactor safety and reactor design" * "Thereotical analysis and numerical modelling of heat transfer and fuel migration in underlying soils and constructive elements of nuclear plants during an accident release from the core" * "How really to attain reactor safely" * "The problem of chemical weapons" * "Long terms genetic effects of nuclear and chemical accidents" * "Features of the brain which are of importance in understanding the mode of operation of toxic substances and of radiation" * "CO2 and ultra safe reactors" * 3. USE OF MISSILES * "How to convert INF technology for peaceful scientific purposes" * "Beating words into plowshares: a proposal for the peaceful uses of retired nuclear warheads" * "Some thoughts on the peaceful use of retired nuclear warheads" * "Status of the HEFEST project" * 4. OZONE * "Status of the ozone layer problem" * 5. CONVENTIONAL AND NUCLEAR FORCE RESTRUCTURING IN EUROPE * 6. CONFLICT AVOIDANCE MODEL * 7. GENERAL DISCUSSION OF THE WORLD LAB PROJECTS * "East-West-North-South Collaboration in Subnuclear Physics" * "Status of the World Lab in the USSR" * CLOSING SESSION

United Nations population estimates and projections with special reference to the Arab world.

PubMed

1980-06-01

The United Nations Population Division has been preparing world population estimates and projections by region since 1951, by country since 1958, and by sex and age for each country since 1968. The latest revision of the projections was prepared in 1978. The 2 basic methods of preparing population projections are mathematical and component, and the component methods are most widely used at present, by both national governments and the United Nations. Before projections are prepared, the base data must be evaluated and adjusted. In the UN projections, the assumptions imply that orderly progress will be made and that there will be no catastrophes such as famines and epidemics during the projection period. The projectins are prepared in 4 variants--"medium", "high," "low," and "constant." A major source of uncertainty in populations arises from the problem of estimating future fertility. Changes in fertility affect the age distribution and the total population size more than changes in mortality. At the UN, mortality assumptions are initially made in terms of life expectancy at birth and then in terms of age-sex patterns of probabilities of survival corresponding to different life expectancy levels at birth. Some of the results of the 1978 revision of the medium variant of the estimates and projections are shown in table form. The world total population of 4,033,000,000 in 1975 is projected to reach 6,199,000,000 by the year 2000. Among the major areas and regions of the world, the most rapid population growth for the future is projected for the Arab countries, Africa and Latin America. Of the 2 Arab regions, North Africa and Southwest Asia, Southwest Asia is expected to have the higher rate of growth because of assumed continued immigration. Within the Arab regions, there has been an increasing diversity in the rate of population growth. This divergence is expected to narrow with assumed decreased migration rates during the 1980s.

Cumulative Causation, Market Transition, and Emigration from China

PubMed Central

Liang, Zai; Chunyu, Miao David; Zhuang, Guotu; Ye, Wenzhen

2009-01-01

In this paper, we report findings from a recent survey of international migration from China's Fujian province to the United States. We take advantage of the ethnosurvey approach as used in the Mexican Migration Project. Surveys were done in migrant-sending communities in China as well as in destination communities of New York City. We derive hypotheses from two strands of recent studies-the international migration literature and the market transition debate. Our results are in general consistent with hypotheses derived from cumulative causation of migration. However, because of the geographical location of China as compared to Mexico, there are some differences between the two countries in terms of particular migration patterns to the United States. As expected, at the community level, migration prevalence ratio (measuring migration networks) increases the propensity of migration for other members in the community. In contrast, having a household member migrated previously does not increase the propensity of migration of other household members until debt for previous migration is paid off. Our research clearly demonstrates the value of bringing the case of China into the comparative study of international migration. With respect to market transition theory, we find that political power continues to be an important factor in the order of social stratification in the coastal Fujian province. PMID:19569396

Migration of epoxidised soybean oil from PVC gaskets of commercial lids: simulation of migration under various conditions and screening of food products from Czech markets.

PubMed

Hanušová, Kristýna; Vrbík, Karel; Rajchl, Aleš; Dobiáš, Jaroslav; Sosnovcová, Jitka

2015-01-01

Previous studies have shown that a large number of polyvinylchloride (PVC) lid gaskets exceed the existing migration limits for epoxidised soybean oil (ESBO) and correct prediction of ESBO release into food therefore appears to be a difficult issue. ESBO migration from PVC gaskets of metal closures into food simulants and food products from the Czech market is evaluated during a survey in 2009 and subsequently one in 2012 to assess progress in lid manufacturing and official testing conditions. ESBO migration from lids into various food simulants was studied at various temperatures (25, 40 and 60°C) during storage times up to 12 months. ESBO released into food simulants or food products was transmethylated, derivatised and analysed by GC-MS. The levels of ESBO migration in foodstuffs in 2012 exceeded the specific migration limit (SML) in fewer products in comparison with the previous survey. However, most of the products were analysed at a time far from the expiry date and exceedance of the SML at the end of the product shelf life is not therefore excluded. More severe test conditions (60°C for 10 days) for specific migration given by the current European Union legislation (Regulation (EU) No. 10/2011) still seem to be insufficient for the simulation of ESBO migration during long-term storage.

High-frequency sediment-level oscillations in the swash zone

USGS Publications Warehouse

Sallenger, A.H.; Richmond, B.M.

1984-01-01

Sediment-level oscillations with heights of about 6 cm and shore-normal lengths of order 10 m have been measured in the swash zone of a high-energy, coarse-sand beach. Crests of oscillations were shore parallel and continuous alongshore. The oscillations were of such low steepness (height-to-length ratio approximately 0.006) that they were difficult to detect visually. The period of oscillation ranged between 6 and 15 min and decreased landward across the swash zone. The sediment-level oscillations were progressive landward with an average migration rate in the middle to upper swash zone of 0.8 m min-1. Migration was caused mostly by erosion on the seaward flank of the crest of an oscillation during a period of net seaward sediment transport. Thus, the observed migration was a form migration landward rather than a migration involving net landward sediment transport. The observed sediment-level oscillations were different than sand waves or other swash-zone bedforms previously described. ?? 1984.

An analysis toolbox to explore mesenchymal migration heterogeneity reveals adaptive switching between distinct modes

PubMed Central

Shafqat-Abbasi, Hamdah; Kowalewski, Jacob M; Kiss, Alexa; Gong, Xiaowei; Hernandez-Varas, Pablo; Berge, Ulrich; Jafari-Mamaghani, Mehrdad; Lock, John G; Strömblad, Staffan

2016-01-01

Mesenchymal (lamellipodial) migration is heterogeneous, although whether this reflects progressive variability or discrete, 'switchable' migration modalities, remains unclear. We present an analytical toolbox, based on quantitative single-cell imaging data, to interrogate this heterogeneity. Integrating supervised behavioral classification with multivariate analyses of cell motion, membrane dynamics, cell-matrix adhesion status and F-actin organization, this toolbox here enables the detection and characterization of two quantitatively distinct mesenchymal migration modes, termed 'Continuous' and 'Discontinuous'. Quantitative mode comparisons reveal differences in cell motion, spatiotemporal coordination of membrane protrusion/retraction, and how cells within each mode reorganize with changed cell speed. These modes thus represent distinctive migratory strategies. Additional analyses illuminate the macromolecular- and cellular-scale effects of molecular targeting (fibronectin, talin, ROCK), including 'adaptive switching' between Continuous (favored at high adhesion/full contraction) and Discontinuous (low adhesion/inhibited contraction) modes. Overall, this analytical toolbox now facilitates the exploration of both spontaneous and adaptive heterogeneity in mesenchymal migration. DOI: http://dx.doi.org/10.7554/eLife.11384.001 PMID:26821527

Copper chaperone Atox1 plays role in breast cancer cell migration.

PubMed

Blockhuys, Stéphanie; Wittung-Stafshede, Pernilla

2017-01-29

Copper (Cu) is an essential transition metal ion required as cofactor in many key enzymes. After cell uptake of Cu, the metal is transported by the cytoplasmic Cu chaperone Atox1 to P 1B -type ATPases in the Golgi network for incorporation into Cu-dependent enzymes in the secretory path. Cu is vital for many steps of cancer progression and Atox1 was recently suggested to have additional functionality as a nuclear transcription factor. We here investigated the expression level, cellular localization and role in cell migration of Atox1 in an aggressive breast cancer cell line upon combining immunostaining, microscopy and a wound healing assay. We made the unexpected discovery that Atox1 accumulates at lamellipodia borders of migrating cancer cells and Atox1 silencing resulted in migration defects as evidenced from reduced wound closure. Therefore, we have discovered an unknown role of the Cu chaperone Atox1 in breast cancer cell migration. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

miR-618 Inhibits Prostate Cancer Migration and Invasion by Targeting FOXP2.

PubMed

Song, Xian-Lu; Tang, Yao; Lei, Xiang-Hui; Zhao, Shan-Chao; Wu, Zi-Qing

2017-01-01

miRNAs play critical role in the development and progression of prostate cancer. Here we studied the role of miR-618 in prostate cancer migration and invasion. miR-618 was downregulated in metastatic androgen-independent prostate cancer (AIPC), patients with low miR-618 had poor outcome. Overexpression of miR-618 inhibited migration and invasion and induced mesenchymal to epithelial transition (MET). Conversely, knockdown of miR-618 promoted migration and invasion and induced epithelial to mesenchymal transition (EMT). FOXP2 was the direct target of miR-618, and promoted TGF-β expression, inhibition of TGF-β reversed the effect of miR-618 knockdown. We further analyzed the correlation between miR-618 expression and FOXP2 in human prostate cancer tissues, and found there was a negative correlation between miR-618 expression and FOXP2 levels. In conclusion, we found miR-618 inhibited prostate cancer migration and invasion by targeting FOXP2 and inhibiting TGF-β.

Overexpression of Rac1 in leukemia patients and its role in leukemia cell migration and growth

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Jiying; Rao, Qing, E-mail: raoqing@gmail.com; Wang, Min

2009-09-04

Rac1 belongs to the Rho family that act as critical mediators of signaling pathways controlling cell migration and proliferation and contributes to the interactions of hematopoietic stem cells with their microenvironment. Alteration of Rac1 might result in unbalanced interactions and ultimately lead to leukemogenesis. In this study, we analyze the expression of Rac1 protein in leukemia patients and determine its role in the abnormal behaviours of leukemic cells. Rac1 protein is overexpressed in primary acute myeloid leukemia cells as compared to normal bone marrow mononuclear cells. siRNA-mediated silencing of Rac1 in leukemia cell lines induced inhibition of cell migration, proliferation,more » and colony formation. Additionally, blocking Rac1 activity by an inhibitor of Rac1-GTPase, NSC23766, suppressed cell migration and growth. We conclude that overexpression of Rac1 contributes to the accelerated migration and high proliferation potential of leukemia cells, which could be implicated in leukemia development and progression.« less

Hungry Horse Dam Fisheries Mitigation : Fish Passage and Habitat Improvement in the Upper Flathead River Basin, 1991-1996 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knotek, W.Ladd; Deleray, Mark; Marotz, Brian L.

1997-08-01

In the past 50 years, dramatic changes have occurred in the Flathead Lake and River system. Degradation of fishery resources has been evident, in part due to deterioration of aquatic habitat and introduction of non-endemic fish and invertebrate species. Habitat loss has been attributed to many factors including the construction and operation of Hungry Horse Dam, unsound land use practices, urban development, and other anthropogenic and natural disturbances. Fish migration has also been limited by barriers such as dams and impassible culverts. Cumulatively, these factors have contributed to declines in the distribution and abundance of native fish populations. Recovery ofmore » fish populations requires that a watershed approach be developed that incorporates long-term aquatic habitat needs and promotes sound land use practices and cooperation among natural resource management agencies. In this document, the authors (1) describe completed and ongoing habitat improvement and fish passage activities under the Hungry Horse Fisheries Mitigation Program, (2) describe recently identified projects that are in the planning stage, and (3) develop a framework for identifying prioritizing, implementing, and evaluating future fish habitat improvement and passage projects.« less

COMPARATIVE ECOLOGICAL STUDIES OF ANIMALS AT THE NEVADA TEST SITE. With Specific Reference to Their Reaction to Exposure of Nuclear Effects. Annual Progress Report covering the Period January 1, 1960 to December 31, 1960

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1962-10-31

A conrparative ecologmcal study of the animals at the Nevada Test Site was made in order to determine their reaction to nuclear explosion effects. To make this study the project was subdivmded mnto the following projects: determine the kmnds and populations of native animals in areas disturbed by atomic explosions in comparison with animals in contiguous undisturbed areas; determine seasonal distribution, migration, home range, and other habits of native animals in disturbed and undisturbed areas; and determine whether there are tissue changes in native animals living in radiation contaminated areas. Results of the program are stmll being analyzed, however, somemore » results are presented. The animals studied primarily are grounddwelling animals, rodents, lizards, ants, grasshoppers, crickets, beetles, spiders, and scorpions. These animals were selected because of their distribution and abundance in many plant communities throughout the test site. Description is given of the 26 major study plots and the techniques used for collecting the animals. 75 supporting plots established and investigated are also described. (N.W.R.)« less

Designing and Assessing Restored Meandering River Planform Using RVR Meander

NASA Astrophysics Data System (ADS)

Langendoen, E. J.; Abad, J. D.; Motta, D.; Frias, C. E.; Wong, M.; Barnes, B. J.; Anderson, C. D.; Garcia, M. H.; MacDonald, T. E.

2013-12-01

The ongoing modification and resulting reduction in water quality of U.S. rivers have led to a significant increase in river restoration projects over the last two decades. The increased interest in restoring degraded streams, however, has not necessarily led to improved stream function. Palmer and Allan (2005) found that many restoration projects fail to achieve their objectives due to the lack of policies to support restoration standards, to promote proven methods and to provide basic data needed for planning and implementation. Proven models of in-stream and riparian processes could be used not only to guide the design of restoration projects but also to assess both pre- and post-project indicators of ecological integrity. One of the most difficult types of river restoration projects concern reconstructing a new channel, often with an alignment and channel form different from those of the degraded pre-project channel. Recreating a meandering planform to provide longitudinal and lateral variability of flow and bed morphology to improve in-stream aquatic habitat is often desired. Channel meander planform is controlled by a multitude of variables, for example channel width to depth ratio, radius of curvature to channel width ratio, bankfull discharge, roughness, bed-material physical characteristics, bed material transport, resistance to erosion of the floodplain soils, riparian vegetation, etc. Therefore, current practices that use simple, empirically based relationships or reference reaches have led to failure in several instances, for example a washing out of meander bends or a highly unstable planform, because they fail to address the site-specific conditions. Recently, progress has been made to enhance a physically- and process-based model, RVR Meander, for rapid analysis of meandering river morphodynamics with reduced empiricism. For example, lateral migration is based on measurable physical properties of the floodplain soils and riparian vegetation versus the driving forces of the river hydrodynamics. The model can also be used in a Monte Carlo framework to statistically describe the long-term evolution of the meander planform. RVR Meander has been successfully used to evaluate migration rates of restored meandering streams and bends on the Big Sioux River, SD and Trout Creek, CA at engineering time scales. It has also been used to assess the uncertainty and risk associated with the alignment of the meandering low-flow channel of the planned diversion of the Red River of the North around the metropolitan area of Fargo, ND and Moorhead, MN. Palmer MA, Allan JD. 2005. Restoring rivers, Issues in Science and Technology, Winter 2006, Published by National Academy of Sciences, 22: 40-48.

Return Migration as Failure or Success?: The Determinants of Return Migration Intentions Among Moroccan Migrants in Europe.

PubMed

de Haas, Hein; Fokkema, Tineke; Fihri, Mohamed Fassi

Different migration theories generate competing hypotheses with regard to determinants of return migration. While neoclassical migration theory associates migration to the failure to integrate at the destination, the new economics of labour migration sees return migration as the logical stage after migrants have earned sufficient assets and knowledge and to invest in their origin countries. The projected return is then likely to be postponed for sustained or indefinite periods if integration is unsuccessful. So, from an indication or result of integration failure return is rather seen as a measure of success. Drawing on recent survey data ( N  = 2,832), this article tests these hypotheses by examining the main determinants of return intention among Moroccan migrants across Europe. The results indicate that structural integration through labour market participation, education and the maintenance of economic and social ties with receiving countries do not significantly affect return intentions. At the same time, investments and social ties to Morocco are positively related, and socio-cultural integration in receiving countries is negatively related to return migration intentions. The mixed results corroborate the idea that there is no uniform process of (return) migration and that competing theories might therefore be partly complementary.

Climate change as a migration driver from rural and urban Mexico

NASA Astrophysics Data System (ADS)

Nawrotzki, Raphael J.; Hunter, Lori M.; Runfola, Daniel M.; Riosmena, Fernando

2015-11-01

Studies investigating migration as a response to climate variability have largely focused on rural locations to the exclusion of urban areas. This lack of urban focus is unfortunate given the sheer numbers of urban residents and continuing high levels of urbanization. To begin filling this empirical gap, this study investigates climate change impacts on US-bound migration from rural and urban Mexico, 1986-1999. We employ geostatistical interpolation methods to construct two climate change indices, capturing warm and wet spell duration, based on daily temperature and precipitation readings for 214 weather stations across Mexico. In combination with detailed migration histories obtained from the Mexican Migration Project, we model the influence of climate change on household-level migration from 68 rural and 49 urban municipalities. Results from multilevel event-history models reveal that a temperature warming and excessive precipitation significantly increased international migration during the study period. However, climate change impacts on international migration is only observed for rural areas. Interactions reveal a causal pathway in which temperature (but not precipitation) influences migration patterns through employment in the agricultural sector. As such, climate-related international migration may decline with continued urbanization and the resulting reductions in direct dependence of households on rural agriculture.

Climate Change as Migration Driver from Rural and Urban Mexico.

PubMed

Nawrotzki, Raphael J; Hunter, Lori M; Runfola, Daniel M; Riosmena, Fernando

2015-11-01

Studies investigating migration as a response to climate variability have largely focused on rural locations to the exclusion of urban areas. This lack of urban focus is unfortunate given the sheer numbers of urban residents and continuing high levels of urbanization. To begin filling this empirical gap, this study investigates climate change impacts on U.S.-bound migration from rural and urban Mexico, 1986-1999. We employ geostatistical interpolation methods to construct two climate change indices, capturing warm and wet spell duration, based on daily temperature and precipitation readings for 214 weather stations across Mexico. In combination with detailed migration histories obtained from the Mexican Migration Project, we model the influence of climate change on household-level migration from 68 rural and 49 urban municipalities. Results from multilevel event-history models reveal that a temperature warming and excessive precipitation significantly increased international migration during the study period. However, climate change impacts on international migration is only observed for rural areas. Interactions reveal a causal pathway in which temperature (but not precipitation) influences migration patterns through employment in the agricultural sector. As such, climate-related international migration may decline with continued urbanization and the resulting reductions in direct dependence of households on rural agriculture.

Migratory neighbors and distant invaders: tumor-associated niche cells

PubMed Central

Wels, Jared; Kaplan, Rosandra N.; Rafii, Shahin; Lyden, David

2008-01-01

The cancer environment is comprised of tumor cells as well as a wide network of stromal and vascular cells participating in the cellular and molecular events necessary for invasion and metastasis. Tumor secretory factors can activate the migration of host cells, both near to and far from the primary tumor site, as well as promote the exodus of cells to distant tissues. Thus, the migration of stromal cells and tumor cells among specialized microenvironments takes place throughout tumor and metastatic progression, providing evidence for the systemic nature of a malignancy. Investigations of the tumor–stromal and stromal–stromal cross-talk involved in cellular migration in cancer may lead to the design of novel therapeutic strategies. PMID:18316475

Social Justice and Mobility in Coastal Louisiana, USA.

PubMed

Colten, Craig E; Simms, Jessica R Z; Grismore, Audrey A; Hemmerling, Scott A

2018-02-01

Louisiana faces extensive coastal land loss which threatens the livelihoods of marginalized populations. These groups have endured extreme disruptive events in the past and have survived in the region by relying on several resilient practices, including mobility. Facing environmental changes that will be wrought by deliberate coastal restoration programs, elderly residents are resisting migration while younger residents continue a decades-long inland migration. Interviews and historical records illustrate a complex intersection of resilient practices and environmental migration. The process underway conflicts to some extent with prevailing concepts in environmental migration most notably deviating from established migration patterns. In terms of social justice, selective out-migration of younger adults leaves a more vulnerable population behind, but also provides a supplementary source of income and social links to inland locales. Organized resistance to restoration projects represents a social justice response to programs that threaten the resource-based livelihoods of coastal residents while offering protection to safer inland urban residents.

Predictors of career progression and obstacles and opportunities for non-EU hospital doctors to undertake postgraduate training in Ireland.

PubMed

Tyrrell, Ella; Keegan, Conor; Humphries, Niamh; McAleese, Sara; Thomas, Steve; Normand, Charles; Brugha, Ruairí

2016-06-30

The World Health Organization's Global Code on the International Recruitment of Health Personnel urges Member States to observe fair recruitment practices and ensure equality of treatment of migrant and domestically-trained health personnel. However, international medical graduates (IMGs) have experienced difficulties in accessing postgraduate training and in progressing their careers in several destination countries. Ireland is highly dependent on IMGs, but also employs non-European Union (EU) doctors who qualified as doctors in Ireland. However, little is known regarding the career progression of these doctors. In this context, the present study assesses the determinants of career progression of non-EU doctors with particular focus on whether barriers to progression exist for those graduating outside Ireland compared to those who have graduated within. The study utilises quantitative data from an online survey of non-EU doctors registered with the Medical Council of Ireland undertaken as part of the Doctor Migration Project (2011-2013). Non-EU doctors registered with the Medical Council of Ireland were asked to complete an online survey about their recruitment, training and career experiences in Ireland. Analysis was conducted on the responses of 231 non-EU hospital doctors whose first post in Ireland was not permanent. Career progression was analysed by means of binary logistic regression analysis. While some of the IMGs had succeeded in accessing specialist training, many experienced slow or stagnant career progression when compared with Irish-trained non-EU doctors. Key predictors of career progression for non-EU doctors working in Ireland showed that doctors who qualified outside of Ireland were less likely than Irish-trained non-EU doctors to experience career progression. Length of stay as a qualified doctor in Ireland was strongly associated with career progression. Those working in anaesthesia were significantly more likely to experience career progression than those in other specialities. The present study highlights differences in terms of achieving career progression and training for Irish-trained non-EU doctors, compared to those trained elsewhere. However, the findings herein warrant further attention from a workforce planning and policy development perspective regarding Ireland's obligations under the Global Code of hiring, promoting and remunerating migrant health personnel on the basis of equality of treatment with the domestically-trained health workforce.

Walla Walla River Fish Passage Operations Program, 2000-2001 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zimmerman, Brian C.; Duke, Bill B.

2004-02-01

In the late 1990's, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow measures, and initiating trap and haul efforts. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adultmore » and juvenile salmonids in the basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2000-2001 project year, there were 624 summer steelhead (Oncorhynchus mykiss), 24 bull trout (Salvelinus confluentus), and 47 spring chinook (O. tshawytscha) counted at the Nursery Bridge Dam adult trap between December 27, 2000 and June 7, 2001. The Little Walla Walla River juvenile trap was not operated this year. The project transported 1600 adult spring chinook from Ringold Springs Hatchery to the South Fork Walla Walla Brood Holding Facility and outplanted 1156 for natural spawning in the basin. The project also provided equipment for transportation of juveniles captured during the construction fish salvage at Nursery Bridge Dam.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rutqvist, Jonny; Blanco-Martin, Laura; Molins, Sergi

In this report, we present FY2015 progress by Lawrence Berkeley National Laboratory (LBNL) related to modeling of coupled thermal-hydrological-mechanical-chemical (THMC) processes in salt and their effect on brine migration at high temperatures. This is a combined milestone report related to milestone Salt R&D Milestone “Modeling Coupled THM Processes and Brine Migration in Salt at High Temperatures” (M3FT-15LB0818012) and the Salt Field Testing Milestone (M3FT-15LB0819022) to support the overall objectives of the salt field test planning.

P.E.O.P.L.E. 32: B.C. College Region Population Projections

ERIC Educational Resources Information Center

Ministry of Advanced Education and Labour Market Development, 2007

2007-01-01

Each year BC Stats, Ministry of Labour and Citizens' Services, projects the population of British Columbia (BC) and its regions. These projections are based on assumptions about future fertility, mortality and migration levels. Assumptions are derived from demographic trends modified to include potential impacts of future economic factors. The…

Predicting and Mapping Potential Whooping Crane Stopover Habitat to Guide Site Selection for Wind Energy Projects

EPA Science Inventory

Migration is one of the most poorly understood components of a bird’s life cycle. For that reason, migratory stopover habitats are often not part of conservation planning and may be overlooked when planning new development projects. This project highlights and addresses an overl...

Assisted migration: What it means to nursery managers and tree planters

Treesearch

Mary I. Williams; R. Kasten Dumroese

2014-01-01

Projections indicate that natural plant adaptation and migration may not keep pace with climate changes. This mismatch in rates will pose significant challenges for practitioners that select, grow, and outplant native tree species. Populations of native tree species planted today must be able to meet the climatic challenges they will face during this century. One...

Floating Migration, Education, and Globalization in the US Caribbean

ERIC Educational Resources Information Center

Gonzalez, Mirerza; Rios-Villarini, Nadjah

2012-01-01

This article follows a research project that collects oral histories of bilingual education teachers from Puerto Rico who migrated to the US Virgin Islands in the late twentieth century. The teachers' oral histories are used as a case study that provides in-depth analysis of competing discourses related to education and globalization in these two…

International Students with Dependent Children: The Reproduction of Gender Norms

ERIC Educational Resources Information Center

Brooks, Rachel

2015-01-01

Extant research on family migration for education has focused almost exclusively on the education of children. We thus know very little about family migration when it is driven by the educational projects of parents. To begin to redress this gap, this paper explores the experiences of families who have moved to the United Kingdom primarily to…

Confederated Tribes Umatilla Indian Reservation (CTUIR) Umatilla Anadromous Fisheries Habitat Project : A Columbia River Basin Fish Habitat Project : Annual Report Fiscal Year 2007.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoverson, Eric D.; Amonette, Alexandra

2008-12-02

The Umatilla Anadromous Fisheries Habitat Project (UAFHP) is an ongoing effort to protect, enhance, and restore riparian and instream habitat for the natural production of anadromous salmonids in the Umatilla River Basin, Northeast Oregon. Flow quantity, water temperature, passage, and lack of in-stream channel complexity have been identified as the key limiting factors in the basin. During the 2007 Fiscal Year (FY) reporting period (February 1, 2007-January 31, 2008) primary project activities focused on improving instream and riparian habitat complexity, migrational passage, and restoring natural channel morphology and floodplain function. Eight fisheries habitat enhancement projects were implemented on Meacham Creek,more » Camp Creek, Greasewood Creek, Birch Creek, West Birch Creek, and the Umatilla River. Specific restoration actions included: (1) rectifying five fish passage barriers on four creeks, (2) planting 1,275 saplings and seeding 130 pounds of native grasses, (3) constructing two miles of riparian fencing for livestock exclusion, (4) coordinating activities related to the installation of two off-channel, solar-powered watering areas for livestock, and (5) developing eight water gap access sites to reduce impacts from livestock. Baseline and ongoing monitoring and evaluation activities were also completed on major project areas such as conducting photo point monitoring strategies activities at the Meacham Creek Large Wood Implementation Project site (FY2006) and at all existing easements and planned project sites. Fish surveys and aquatic habitat inventories were conducted at project sites prior to implementation. Monitoring plans will continue throughout the life of each project to oversee progression and inspire timely managerial actions. Twenty-seven conservation easements were maintained with 23 landowners. Permitting applications for planned project activities and biological opinions were written and approved. Project activities were based on a variety of fisheries monitoring techniques and habitat assessments used to determine existing conditions and identify factors limiting anadromous salmonid abundance. Proper selection and implementation of the most effective site-specific habitat restoration plan, taking into consideration the unique characteristics of each project site, and conducted in cooperation with landowners and project partners, was of paramount importance to ensure each project's success.« less

The multiple faces of leukocyte interstitial migration

PubMed Central

Lämmermann, Tim; Germain, Ronald N.

2014-01-01

Spatiotemporal control of leukocyte dynamics within tissues is critical for successful innate and adaptive immune responses. Homeostatic trafficking and coordinated infiltration into and within sites of inflammation and infection rely on signaling in response to extracellular cues that in turn controls a variety of intracellular protein networks regulating leukocyte motility, migration, chemotaxis, positioning, and cell–cell interaction. In contrast to mesenchymal cells, leukocytes migrate in an amoeboid fashion by rapid cycles of actin polymerization and actomyosin contraction, and their migration in tissues is generally referred to as low adhesive and nonproteolytic. The interplay of actin network expansion, contraction, and adhesion shapes the exact mode of amoeboid migration, and in this review, we explore how leukocyte subsets potentially harness the same basic biomechanical mechanisms in a cell-type-specific manner. Most of our detailed understanding of these processes derives from in vitro migration studies in three-dimensional gels and confined spaces that mimic geometrical aspects of physiological tissues. We summarize these in vitro results and then critically compare them to data from intravital imaging of leukocyte interstitial migration in mouse tissues. We outline the technical challenges of obtaining conclusive mechanistic results from intravital studies, discuss leukocyte migration strategies in vivo, and present examples of mode switching during physiological interstitial migration. These findings are also placed in the context of leukocyte migration defects in primary immunodeficiencies. This overview of both in vitro and in vivo studies highlights recent progress in understanding the molecular and biophysical mechanisms that shape robust leukocyte migration responses in physiologically complex and heterogeneous environments. PMID:24573488

Final Project Report: Imaging Fault Zones Using a Novel Elastic Reverse-Time Migration Imaging Technique

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang, Lianjie; Chen, Ting; Tan, Sirui

Imaging fault zones and fractures is crucial for geothermal operators, providing important information for reservoir evaluation and management strategies. However, there are no existing techniques available for directly and clearly imaging fault zones, particularly for steeply dipping faults and fracture zones. In this project, we developed novel acoustic- and elastic-waveform inversion methods for high-resolution velocity model building. In addition, we developed acoustic and elastic reverse-time migration methods for high-resolution subsurface imaging of complex subsurface structures and steeply-dipping fault/fracture zones. We first evaluated and verified the improved capabilities of our newly developed seismic inversion and migration imaging methods using synthetic seismicmore » data. Our numerical tests verified that our new methods directly image subsurface fracture/fault zones using surface seismic reflection data. We then applied our novel seismic inversion and migration imaging methods to a field 3D surface seismic dataset acquired at the Soda Lake geothermal field using Vibroseis sources. Our migration images of the Soda Lake geothermal field obtained using our seismic inversion and migration imaging algorithms revealed several possible fault/fracture zones. AltaRock Energy, Inc. is working with Cyrq Energy, Inc. to refine the geologic interpretation at the Soda Lake geothermal field. Trenton Cladouhos, Senior Vice President R&D of AltaRock, was very interested in our imaging results of 3D surface seismic data from the Soda Lake geothermal field. He planed to perform detailed interpretation of our images in collaboration with James Faulds and Holly McLachlan of University of Nevada at Reno. Using our high-resolution seismic inversion and migration imaging results can help determine the optimal locations to drill wells for geothermal energy production and reduce the risk of geothermal exploration.« less

MiR-26b inhibits hepatocellular carcinoma cell proliferation, migration, and invasion by targeting EphA2.

PubMed

Li, Hesheng; Sun, Qinglei; Han, Bing; Yu, Xingquan; Hu, Baoguang; Hu, Sanyuan

2015-01-01

Deregulated microRNAs (miRNAs) have been shown to play important roles in cancer progression as a result of changes in expression of their target genes. In this study, we investigated the expression of miR-16b in eight hepatocellular carcinoma (HCC) cell lines, revealed the roles of miR-26b on hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion, and confirmed that EphA2 is a direct target of miR-26b. The miR-26b expression was decreased and EphA2 expression was evaluated in HCC cell lines. Luciferase assays revealed that miR-26b inhibited EphA2 expression by targeting the 3'-untranslated region of EphA2 mRNA. Overexpression of miR-26b dramatically inhibited the proliferation, invasion, and migration of HCC cells by targeting EphA2. Moreover, miR-26b down-regulated c-Myc and CyclinD1 expression, which was reversed by overexpressed EphA2. Taken together, our data demonstrated the mechanism of miR-26b contributed to HCC progression and implicated that miR-26b's potential in HCC therapy.

microRNA-625 inhibits tumorigenicity by suppressing proliferation, migration and invasion in malignant melanoma.

PubMed

Fang, Wei; Fan, Yibin; Fa, Zhenzong; Xu, Jinhua; Yu, Hongyu; Li, Pu; Gu, Julin

2017-02-21

Dysregulated microRNA (miR)-625 expression has been observed in several kinds of cancer. MicroRNAs are important factors in the development and progression of malignant melanoma, though the clinical significance and function of miR-625 in human malignant melanoma remain unclear. Levels of miR-625 expression were therefore determined in 36 pairs of malignant melanoma and adjacent non-tumor tissue using qPCR. The effects of miR-625 dysregulation on malignant melanoma cell proliferation, wound healing, migration and invasion in vitro and tumorigenicity in vivo were investigated using CCK-8, transwell assays, and a nude mouse subcutaneous tumor model. Bioinformatics analysis and luciferase reporter system were used to predict and confirm the target gene of miR-625. miR-625 levels were frequently decreased in malignant melanoma. Ectopic expression of miR-625 suppressed proliferation, wound healing, migration, and tumorgenicity in malignant melanoma. Moreover, miR-625 acted, at least in part, by suppressing potential target SOX2. These results show that miR-625 is a tumor suppressor that inhibits the development and progression of malignant melanoma, which suggests miR-625 is potentially a new diagnostic marker and therapeutic target of malignant melanoma.

Interactions between CXCR4 and CXCL12 promote cell migration and invasion of canine hemangiosarcoma.

PubMed

Im, K S; Graef, A J; Breen, M; Lindblad-Toh, K; Modiano, J F; Kim, J-H

2017-06-01

The CXCR4/CXCL12 axis plays an important role in cell locomotion and metastasis in many cancers. In this study, we hypothesized that the CXCR4/CXCL12 axis promotes migration and invasion of canine hemangiosarcoma (HSA) cells. Transcriptomic analysis across 12 HSA cell lines and 58 HSA whole tumour tissues identified heterogeneous expression of CXCR4 and CXCL12, which was associated with cell movement. In vitro, CXCL12 promoted calcium mobilization, cell migration and invasion that were directly proportional to surface expression of CXCR4; furthermore, these responses proved sensitive to the CXCR4 antagonist, AMD3100, in HSA cell lines. These results indicate that CXCL12 potentiates migration and invasion of canine HSA cells through CXCR4 signalling. The direct relationship between these responses in HSA cells suggests that the CXCR4/CXCL12 axis contributes to HSA progression. © 2015 John Wiley & Sons Ltd.

TRPV2 Mediates Adrenomedullin Stimulation of Prostate and Urothelial Cancer Cell Adhesion, Migration and Invasion

PubMed Central

Vanden Abeele, Fabien; Lehen’kyi, V’yacheslav; Ouafik, L’Houcine; Mauroy, Brigitte; Prevarskaya, Natalia

2013-01-01

Adrenomedullin (AM) is a 52-amino acid peptide initially isolated from human pheochromocytoma. AM is expressed in a variety of malignant tissues and cancer cell lines and was shown to be a mitogenic factor capable of stimulating growth of several cancer cell types. In addition, AM is a survival factor for certain cancer cells. Some data suggest that AM might be involved in the progression cancer metastasis via angiogenesis and cell migration and invasion control. The Transient Receptor Potential channel TRPV2 is known to promote in prostate cancer cell migration and invasive phenotype and is correlated with the stage and grade of bladder cancer. In this work we show that AM induces prostate and urothelial cancer cell migration and invasion through TRPV2 translocation to plasma membrane and the subsequent increase in resting calcium level. PMID:23741410

[Phagocyte migration: an overview].

PubMed

Le Cabec, Véronique; Van Goethem, Emeline; Guiet, Romain; Maridonneau-Parini, Isabelle

2011-12-01

Phagocytes are the first line of host defense thanks to their capacity to infiltrate infected and wounded tissues, where they exert their bactericidal and tissue repair functions. However, tissue infiltration of phagocytes also stimulates the progression of pathologies such as cancer and chronic inflammatory diseases. It is therefore necessary to identify the molecular and cellular mechanisms that control this process to identify new therapeutic targets. Phagocytes leave the blood stream by crossing the vascular wall and infiltrate interstitial tissues, a three-dimensional environment. A state-of-the-art of the different steps of phagocyte tissue recruitment in vivo and of the different in vitro models is developed in this synthesis. We focus on recent data concerning the migration of phagocytes in three-dimensional environments. The use of two different migration modes, amoeboid and mesenchymal, by macrophages and the role of podosomes and proteases in the mesenchymal migration are discussed. © 2011 médecine/sciences – Inserm / SRMS.

Aberrant Notch1-dependent effects on glomerular parietal epithelial cells promotes collapsing focal segmental glomerulosclerosis with progressive podocyte loss.

PubMed

Ueno, Toshiharu; Kobayashi, Namiko; Nakayama, Makiko; Takashima, Yasutoshi; Ohse, Takamoto; Pastan, Ira; Pippin, Jeffrey W; Shankland, Stuart J; Uesugi, Noriko; Matsusaka, Taiji; Nagata, Michio

2013-06-01

Collapsing focal segmental glomerulosclerosis (cFSGS) is a progressive kidney disease characterized by glomerular collapse with epithelial hyperplasia. Here we used a transgenic mouse model of cFSGS with immunotoxin-induced podocyte-specific injury to determine the role for Notch signaling in its pathogenesis. The mice exhibited progressive loss of podocytes and severe proteinuria concomitant with histological features of cFSGS. Hyperplastic epithelium was negative for genetic podocyte tags, but positive for the parietal epithelial cell marker claudin-1, and expressed Notch1, Jagged1, and Hes1 mRNA and protein. Enhanced Notch mRNA expression induced by transforming growth factor-β1 in cultured parietal epithelial cells was associated with mesenchymal markers (α-smooth muscle actin, vimentin, and Snail1). Notch inhibition in vitro suppressed these phenotypic transcripts and Notch-dependent cell migration. Moreover, Notch inhibition in vivo significantly decreased parietal epithelial cell lesions but worsened proteinuria and histopathology in our cFSGS model. Thus, aberrant Notch1-mediated parietal epithelial cell migration with phenotypic changes appears to underlie the pathogenesis of cFSGS. Parietal epithelial cell hyperplasia may also represent an adaptive response to compensate for a disrupted filtration barrier with progressive podocyte loss.

White Adipose Tissue Cells Are Recruited by Experimental Tumors and Promote Cancer Progression in Mouse Models

PubMed Central

Zhang, Yan; Daquinag, Alexes; Traktuev, Dmitry O.; Amaya-Manzanares, Felipe; Simmons, Paul J.; March, Keith L.; Pasqualini, Renata; Arap, Wadih; Kolonin, Mikhail G.

2010-01-01

The connection between obesity and accelerated cancer progression has been established, but the mediating mechanisms are not well understood. We have shown that stromal cells from white adipose tissue (WAT) cooperate with the endothelium to promote blood vessel formation through the secretion of soluble trophic factors. Here, we hypothesize that WAT directly mediates cancer progression by serving as a source of cells that migrate to tumors and promote neovascularization. To test this hypothesis, we have evaluated the recruitment of WAT-derived cells by tumors and the effect of their engraftment on tumor growth by integrating a transgenic mouse strain engineered for expansion of traceable cells with established allograft and xenograft cancer models. Our studies show that entry of adipose stromal and endothelial cells into systemic circulation leads to their homing to and engraftment into tumor stroma and vasculature, respectively. We show that recruitment of adipose stromal cells by tumors is sufficient to promote tumor growth. Finally, we show that migration of stromal and vascular progenitor cells from WAT grafts to tumors is also associated with acceleration of cancer progression. These results provide a biological insight for the clinical association between obesity and cancer, thus outlining potential avenues for preventive and therapeutic strategies. PMID:19491274

Migration, social structure and old-age support networks: a comparison of three Indonesian communities

PubMed Central

KREAGER, PHILIP

2007-01-01

Contemporary trends in population ageing and urbanisation in the developing world imply that the extensive out-migration of young people from rural areas coincides with, and is likely to exacerbate, a rise in the older share of the rural population. This paper examines the impact of migration on vulnerability at older ages by drawing on the results of anthropological and demographic field studies in three Indonesian communities. The methodology for identifying vulnerable older people has a progressively sharper focus, beginning first with important differences between the communities, then examining variations by socio-economic strata, and finally the variability of older people's family networks. Comparative analysis indicates considerable heterogeneity in past and present migration patterns, both within and between villages. The migrants' contributions are a normal and important component of older people's support, often in combination with those of local family members. Higher status families are commonly able to reinforce their position by making better use of migration opportunities than the less advantaged. Although family networks in the poorer strata may effect some redistribution of the children's incomes, their social networks are smaller and insufficient to overcome their marked disadvantages. Vulnerability thus arises where several factors, including migration histories, result in unusually small networks, and when the migrations are within rural areas. PMID:23750063

Directional cell migration in an extracellular pH gradient: a model study with an engineered cell line and primary microvascular endothelial cells.

PubMed

Paradise, Ranjani K; Whitfield, Matthew J; Lauffenburger, Douglas A; Van Vliet, Krystyn J

2013-02-15

Extracellular pH (pH(e)) gradients are characteristic of tumor and wound environments. Cell migration in these environments is critical to tumor progression and wound healing. While it has been shown previously that cell migration can be modulated in conditions of spatially invariant acidic pH(e) due to acid-induced activation of cell surface integrin receptors, the effects of pH(e) gradients on cell migration remain unknown. Here, we investigate cell migration in an extracellular pH(e) gradient, using both model α(v)β(3) CHO-B2 cells and primary microvascular endothelial cells. For both cell types, we find that the mean cell position shifts toward the acidic end of the gradient over time, and that cells preferentially polarize toward the acidic end of the gradient during migration. We further demonstrate that cell membrane protrusion stability and actin-integrin adhesion complex formation are increased in acidic pH(e), which could contribute to the preferential polarization toward acidic pH(e) that we observed for cells in pH(e) gradients. These results provide the first demonstration of preferential cell migration toward acid in a pH(e) gradient, with intriguing implications for directed cell migration in the tumor and wound healing environments. Copyright © 2012 Elsevier Inc. All rights reserved.

Overexpression of Lin28 inhibits the proliferation, migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells.

PubMed

Song, Hu; Xu, Wei; Song, Jun; Liang, Yong; Fu, Wei; Zhu, Xiao-Cheng; Li, Chao; Peng, Jun-Sheng; Zheng, Jun-Nian

2015-02-01

Lin28 plays important roles in the development, maintenance of pluripotency and progression of various types of cancers. Lin28 represses the biogenesis of let-7 microRNAs and is implicated in both development and tumorigenesis. Oncogenic regulation of let-7 microRNAs has been demonstrated in several human malignancies, yet their correlation with Lin28 has not yet been studied in gastric cancer. Therefore, in the present study, we explored the possible mechanisms involved in the effects by Lin28 on the proliferation, migration, cell cycle arrest and apoptosis in gastric cancer cells via alteration of let-7 miRNA. The expression levels of Lin28 and let-7 were detected by real-time PCR in gastric cancer cell lines in vitro. Lin28 was overexpressed in the BGC-823 cells via lentiviral transfection, and let-7 expression was assessed. Cell proliferation and migration capabilities were investigated by MTT and Transwell assays, while cell cycle distribution and the apoptosis rate were detected using flow cytometry. The expression of Lin28 was moderately expressed in the GES cells while underexpressed in the BGC-823, SGC-7901 and HGC-27 cells. Let-7a miRNA was highly expressed in the GES, BGC-823, SGC-7901 and HGC-27 cells. Overexpression of Lin28 was inversely correlated with the downregulated expression of let-7a, and markedly suppressed the proliferation, migration, cell cycle progression and induced apoptosis in the BGC-823 cells. These findings demonstrated that overexpression of Lin28 can suppress the biological behavior of gastric cancer in vitro, and let-7 miRNA may play an important role in the process. We suggest that Lin28 may be a candidate predictor or an anticancer therapeutic target for gastric cancer patients.

Fluctuations in Blood Marginal Zone B-Cell Frequencies May Reflect Migratory Patterns Associated with HIV-1 Disease Progression Status

PubMed Central

Poudrier, Johanne; Roger, Michel

2016-01-01

We have previously shown that overexpression of BLyS/BAFF was associated with increased relative frequencies of innate “precursor” marginal zone (MZ)-like B-cells in the blood of HIV-1-infected rapid and classic progressors. However, along with relatively normal BLyS/BAFF expression levels, these cells remain unaltered in elite-controllers (EC), rather, percentages of more mature MZ-like B-cells are decreased in the blood of these individuals. Fluctuations in frequencies of blood MZ-like B-cell populations may reflect migratory patterns associated with disease progression status, suggesting an important role for these cells in HIV-1 pathogenesis. We have therefore longitudinally measured plasma levels of B-tropic chemokines by ELISA-based technology as well as their ligands by flow-cytometry on blood B-cell populations of HIV-1-infected individuals with different rates of disease progression and uninfected controls. Migration potential of B-cell populations from these individuals were determined by chemotaxis assays. We found important modulations of CXCL13-CXCR5, CXCL12-CXCR4/CXCR7, CCL20-CCR6 and CCL25-CCR9 chemokine-axes and increased cell migration patterns in HIV progressors. Interestingly, frequencies of CCR6 expressing cells were significantly elevated within the precursor MZ-like population, consistent with increased migration in response to CCL20. Although we found little modulation of chemokine-axes in EC, cell migration was greater than that observed for uninfected controls, especially for MZ-like B-cells. Overall the immune response against HIV-1 may involve recruitment of MZ-like B-cells to peripheral sites. Moreover, our findings suggest that “regulated” attraction of these cells in a preserved BLyS/BAFF non-inflammatory environment, such as encountered in EC could be beneficial to the battle and even control of HIV. PMID:27203285

Nurse Migration: A Canadian Case Study

PubMed Central

Little, Lisa

2007-01-01

Objective To synthesize information about nurse migration in and out of Canada and analyze its role as a policy lever to address the Canadian nursing shortage. Principal Findings Canada is both a source and a destination country for international nurse migration with an estimated net loss of nurses. The United States is the major beneficiary of Canadian nurse emigration resulting from the reduction of full-time jobs for nurses in Canada due to health system reforms. Canada faces a significant projected shortage of nurses that is too large to be ameliorated by ethical international nurse recruitment and immigration. Conclusions The current and projected shortage of nurses in Canada is a product of health care cost containment policies that failed to take into account long-term consequences for nurse workforce adequacy. An aging nurse workforce, exacerbated by layoffs of younger nurses with less seniority, and increasing demand for nurses contribute to a projection of nurse shortage that is too great to be solved ethically through international nurse recruitment. National policies to increase domestic nurse production and retention are recommended in addition to international collaboration among developed countries to move toward greater national nurse workforce self sufficiency. PMID:17489918

Measuring research progress in photovoltaics

NASA Technical Reports Server (NTRS)

Jackson, B.; Mcguire, P.

1986-01-01

The role and some results of the project analysis and integration function in the Flat-plate Solar Array (FSA) Project are presented. Activities included supporting the decision-making process, preparation of plans for project direction, setting goals for project activities, measuring progress within the project, and the development and maintenance of analytical models.

Chemoattractant signaling between tumor cells and macrophages regulates cancer cell migration, metastasis and neovascularization.

PubMed

Green, Chad E; Liu, Tiffany; Montel, Valerie; Hsiao, Gene; Lester, Robin D; Subramaniam, Shankar; Gonias, Steven L; Klemke, Richard L

2009-08-21

Tumor-associated macrophages are known to influence cancer progression by modulation of immune function, angiogenesis, and cell metastasis, however, little is known about the chemokine signaling networks that regulate this process. Utilizing CT26 colon cancer cells and RAW 264.7 macrophages as a model cellular system, we demonstrate that treatment of CT26 cells with RAW 264.7 conditioned medium induces cell migration, invasion and metastasis. Inflammatory gene microarray analysis indicated CT26-stimulated RAW 264.7 macrophages upregulate SDF-1alpha and VEGF, and that these cytokines contribute to CT26 migration in vitro. RAW 264.7 macrophages also showed a robust chemotactic response towards CT26-derived chemokines. In particular, microarray analysis and functional testing revealed CSF-1 as the major chemoattractant for RAW 264.7 macrophages. Interestingly, in the chick CAM model of cancer progression, RAW 264.7 macrophages localized specifically to the tumor periphery where they were found to increase CT26 tumor growth, microvascular density, vascular disruption, and lung metastasis, suggesting these cells home to actively invading areas of the tumor, but not the hypoxic core of the tumor mass. In support of these findings, hypoxic conditions down regulated CSF-1 production in several tumor cell lines and decreased RAW 264.7 macrophage migration in vitro. Together our findings suggest a model where normoxic tumor cells release CSF-1 to recruit macrophages to the tumor periphery where they secrete motility and angiogenic factors that facilitate tumor cell invasion and metastasis.

Long Non-Coding RNA MALAT1 Interacts With miR-204 to Modulate Human Hilar Cholangiocarcinoma Proliferation, Migration, and Invasion by Targeting CXCR4.

PubMed

Tan, Xinyu; Huang, Zhiguo; Li, Xiaogang

2017-11-01

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is involved in the development and progression of many types of tumors. An aberrant expression of MALAT1 was observed in many kinds of cancers. However, the exact effects and molecular mechanisms of MALAT1 in human hilar cholangiocarcinoma (HCCA) progression are still unknown. Here, we investigated the role of MALAT1 in human HCCA cell lines and clinical tumor samples in order to determine the function of this lncRNA. In our research, lncRNA-MALAT1 was specifically upregulated in HCCA tissues and cell lines, and was associated with pathological T stage, a larger tumor size, and perineural invasion. Knockdown of MALAT1 inhibited the proliferation, migration, and invasion of human HCCA cell. In addition, chemokine receptor-4 (CXCR4) was involved in MALAT1 induced human HCCA growth, migration, and invasion. By using online tools and a series of mechanistic analysis, we also demonstrated that miR-204-dependent CXCR4 regulation was required in MALAT1 modulating HCCA cell growth, migration and invasion. Taken together, our data indicated that MALAT1 might play an oncogenic role in HCCA through miR-204-dependent CXCR4 regulation, and could be regarded as a therapeutic target in HCCA. J. Cell. Biochem. 118: 3643-3653, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Climate Change as Migration Driver from Rural and Urban Mexico

PubMed Central

Hunter, Lori M.; Runfola, Daniel M.; Riosmena, Fernando

2015-01-01

Studies investigating migration as a response to climate variability have largely focused on rural locations to the exclusion of urban areas. This lack of urban focus is unfortunate given the sheer numbers of urban residents and continuing high levels of urbanization. To begin filling this empirical gap, this study investigates climate change impacts on U.S.-bound migration from rural and urban Mexico, 1986–1999. We employ geostatistical interpolation methods to construct two climate change indices, capturing warm and wet spell duration, based on daily temperature and precipitation readings for 214 weather stations across Mexico. In combination with detailed migration histories obtained from the Mexican Migration Project, we model the influence of climate change on household-level migration from 68 rural and 49 urban municipalities. Results from multilevel event-history models reveal that a temperature warming and excessive precipitation significantly increased international migration during the study period. However, climate change impacts on international migration is only observed for rural areas. Interactions reveal a causal pathway in which temperature (but not precipitation) influences migration patterns through employment in the agricultural sector. As such, climate-related international migration may decline with continued urbanization and the resulting reductions in direct dependence of households on rural agriculture. PMID:26692890

Interdisciplinary investigations in support of project DI-MOD

NASA Technical Reports Server (NTRS)

Starks, Scott A.

1991-01-01

Interdisciplinary investigations in support of project DI-MOD are discussed. The following subject areas were covered: (1) potential extensions of Project DI-MOD to additional sites in Central America; (2) human migration patterns and their impact on malaria transmission; and (3) an investigation into possible computer-based approaches to the analysis of remotely sensed multispectral data.

Quantitative image analysis for investigating cell-matrix interactions

NASA Astrophysics Data System (ADS)

Burkel, Brian; Notbohm, Jacob

2017-07-01

The extracellular matrix provides both chemical and physical cues that control cellular processes such as migration, division, differentiation, and cancer progression. Cells can mechanically alter the matrix by applying forces that result in matrix displacements, which in turn may localize to form dense bands along which cells may migrate. To quantify the displacements, we use confocal microscopy and fluorescent labeling to acquire high-contrast images of the fibrous material. Using a technique for quantitative image analysis called digital volume correlation, we then compute the matrix displacements. Our experimental technology offers a means to quantify matrix mechanics and cell-matrix interactions. We are now using these experimental tools to modulate mechanical properties of the matrix to study cell contraction and migration.

The Short-Term Impact of Involuntary Migration in China's Three Gorges: A Prospective Study

ERIC Educational Resources Information Center

Hwang, Sean-Shong; Cao, Yue; Xi, Juan

2011-01-01

The aim of this study is to measure the short-term impact of involuntary migration resulting from China's Three Gorges Dam project on the 1.3 million persons being displaced. We focus on the social, economic, and mental and physical health impact using three sets of indicators. Using a prospective research design, we gathered information about…

Climate of migration? How climate triggered migration from southwest Germany to North America during the 19th century

NASA Astrophysics Data System (ADS)

Glaser, Rüdiger; Himmelsbach, Iso; Bösmeier, Annette

2017-11-01

This paper contributes to the ongoing debate on the extent to which climate and climatic change can have a negative impact on societies by triggering migration, or even contribute to conflict. It summarizes results from the transdisciplinary project Climate of migration (funded 2010-2014), whose innovative title was created by Franz Mauelshagen and Uwe Lübken. The overall goal of this project was to analyze the relation between climatic and socioeconomic parameters and major migration waves from southwest Germany to North America during the 19th century. The article assesses the extent to which climatic conditions triggered these migration waves. The century investigated was in general characterized by the Little Ice Age with three distinct cooling periods, causing major glacier advances in the alpine regions and numerous climatic extremes such as major floods, droughts and severe winter. Societal changes were tremendous, marked by the warfare during the Napoleonic era (until 1815), the abolition of serfdom (1817), the bourgeois revolution (1847/48), economic freedom (1862), the beginning of industrialization accompanied by large-scale rural-urban migration resulting in urban poverty, and finally by the foundation of the German Empire in 1871.

The presented study is based on quantitative data and a qualitative, information-based discourse analysis. It considers climatic conditions as well as socioeconomic and political issues, leading to the hypothesis of a chain of effects ranging from unfavorable climatic conditions to a decrease in crop yields to rising cereal prices and finally to emigration. These circumstances were investigated extensively for the peak emigration years identified with each migration wave. Furthermore, the long-term relations between emigration and the prevailing climatic conditions, crop yields and cereal prices were statistically evaluated with a sequence of linear models which were significant with explanatory power between 22 and 38 %.

Projected Impacts of 21st Century Climate Change on Potential Habitat for Vegetation and Forest Types in Russia

NASA Astrophysics Data System (ADS)

Soja, A. J.; Tchebakova, N. M.; Parfenova, E. I.; Cantin, A.; Conard, S. G.

2015-12-01

Global GCMs have demonstrated profound potential for projections to affect the distribution of terrestrial ecosystems and individual species at all hierarchical levels. We modeled progression of potential Russian ecotones and forest-forming species as the climate changes. Large-scale bioclimatic models were developed to predict Russian zonal vegetation (RuBCliM) and forest types (ForCliM) from three bioclimatic indices (1) growing degree-days above 5 degrees C; (2) negative degree-days below 0 C ; and (3) an annual moisture index (ratio of growing degree days to annual precipitation). The presence or absence of continuous permafrost was explicitly included in the models as limiting the forests and tree species distribution. All simulations to predict vegetation change across Russia were run by coupling our bioclimatic models with bioclimatic indices and the permafrost distribution for the baseline period and for the future 2020, 2050 and 2100 simulated by 3 GCMs (CGCM3.1, HadCM3 and IPSLCM4) and 3 climate change scenarios (A1B, A2 and B1). Under these climate scenarios, it is projected the zonobiomes will shift far northward to reach equilibrium with the change in climate. Under the warmer and drier projected future climate, about half of Russia would be suitable for the forest-steppe ecotone and grasslands, rather than for forests. Water stress tolerant light-needled taiga would have an increased advantage over water-loving dark-needled taiga. Permafrost-tolerant L. dahurica taiga would remain the dominant forest across permafrost. Increases in severe fire weather would lead to increases in large, high-severity fires, especially at boundaries between forest ecotones, which can be expected to facilitate a more rapid progression of vegetation towards a new equilibrium with the climate. Adaptation to climate change may be facilitated by: assisting migration of forests by seed transfers to establish genotypes that may be more ecologically suited as climate changes; and the introduction of suitable agricultural crops that may be potentially adapted to a warmer climate in the expected steppe and forest-steppe.

Emergence of long distance bird migrations: a new model integrating global climate changes

NASA Astrophysics Data System (ADS)

Louchart, Antoine

2008-12-01

During modern birds history, climatic and environmental conditions have evolved on wide scales. In a continuously changing world, landbirds annual migrations emerged and developed. However, models accounting for the origins of these avian migrations were formulated with static ecogeographic perspectives. Here I reviewed Cenozoic paleoclimatic and paleontological data relative to the palearctic paleotropical long distance (LD) migration system. This led to propose a new model for the origin of LD migrations, the ‘shifting home’ model (SHM). It is based on a dynamic perspective of climate evolution and may apply to the origins of most modern migrations. Non-migrant tropical African bird taxa were present at European latitudes during most of the Cenozoic. Their distribution limits shifted progressively toward modern tropical latitudes during periods of global cooling and increasing seasonality. In parallel, decreasing winter temperatures in the western Palearctic drove shifts of population winter ranges toward the equator. I propose that this induced the emergence of most short distance migrations, and in turn LD migrations. This model reconciliates ecologically tropical ancestry of most LD migrants with predominant winter range shifts, in accordance with requirements for heritable homing. In addition, it is more parsimonious than other non-exclusive models. Greater intrinsic plasticity of winter ranges implied by the SHM is supported by recently observed impacts of the present global warming on migrating birds. This may induce particular threats to some LD migrants. The ancestral, breeding homes of LD migrants were not ‘northern’ or ‘southern’ but shifted across high and middle latitudes while migrations emerged through winter range shifts themselves.

Quantifying Process-Based Mitigation Strategies in Historical Context: Separating Multiple Cumulative Effects on River Meander Migration

PubMed Central

Fremier, Alexander K.; Girvetz, Evan H.; Greco, Steven E.; Larsen, Eric W.

2014-01-01

Environmental legislation in the US (i.e. NEPA) requires defining baseline conditions on current rather than historical ecosystem conditions. For ecosystems with long histories of multiple environmental impacts, this baseline method can subsequently lead to a significantly altered environment; this has been termed a ‘sliding baseline’. In river systems, cumulative effects caused by flow regulation, channel revetment and riparian vegetation removal significantly impact floodplain ecosystems by altering channel dynamics and precluding subsequent ecosystem processes, such as primary succession. To quantify these impacts on floodplain development processes, we used a model of river channel meander migration to illustrate the degree to which flow regulation and riprap impact migration rates, independently and synergistically, on the Sacramento River in California, USA. From pre-dam conditions, the cumulative effect of flow regulation alone on channel migration is a reduction by 38%, and 42–44% with four proposed water diversion project scenarios. In terms of depositional area, the proposed water project would reduce channel migration 51–71 ha in 130 years without current riprap in place, and 17–25 ha with riprap. Our results illustrate the utility of a modeling approach for quantifying cumulative impacts. Model-based quantification of environmental impacts allow scientists to separate cumulative and synergistic effects to analytically define mitigation measures. Additionally, by selecting an ecosystem process that is affected by multiple impacts, it is possible to consider process-based mitigation scenarios, such as the removal of riprap, to allow meander migration and create new floodplains and allow for riparian vegetation recruitment. PMID:24964145

Rural development and urban migration: can we keep them down on the farm?

PubMed

Rhoda, R

1983-01-01

This study tests the hypothesis that rural development projects and programs reduce rural-urban migration. The author presents various factors in the social theories of migration, including those relating to origin and destination, intervening obstacles such as distance, and personal factors. 3 economic models of migration are the human capital or cost-benefit approach, the expected income model, and the intersectoral linkage model. Empirical studies of migration indicate that: 1) rural areas with high rates of out-migration tend to have high population densities or high ratios of labor to arable land, 2) distance inhibits migration, 3) rural-urban migration is positively correlated with family income level, and 4) selectivity differences in socioeconomic status between migrants and nonmigrants often are grouped into development packages which might include irrigation, new varieties of seed, subsidized credit, increased extension, and improved marketing arrangements. The migration impacts of some of these efforts are described: 1) land reform usually is expected to slow rural out-migration because it normally increases labor utilization in rural areas, but this is a limited effect, 2) migration effects of the Green Revolution technology are mainly in rural-rural migration, and 3) agricultural mechanization may stimulate rural-urban migration in the long run. Development of rural social services migh have various effects on rural-urban migration. Better rural education, which improves the chances of urban employment, will stimulate rural-urban migration, while successful rural family planning programs will have a negative effect in the long run as there will be reduced population pressure on arable land. Better rural health services might reduce the incentive for rural-urban migration as well. It is suggested that governments reconsider policies which rely on rural development to curb rural-urban migration and alleviate problems of urban poverty and underemployment.

Pathogenic role for macrophage migration inhibitory factor in glioblastoma and its targeting with specific inhibitors as novel tailored therapeutic approach

PubMed Central

Mangano, Katia; Mazzon, Emanuela; Basile, Maria Sofia; Di Marco, Roberto; Bramanti, Placido; Mammana, Santa; Petralia, Maria Cristina; Fagone, Paolo; Nicoletti, Ferdinando

2018-01-01

Macrophage Migration Inhibitory Factor (MIF) is a pro-inflammatory cytokine expressed by a variety of cell types. Although MIF has been primarily studied for its role in the pathogenesis of autoimmune diseases, it has also been shown to promote tumorigenesis and it is over expressed in various malignant tumors. MIF is able to induce angiogenesis, cell cycle progression, and to block apoptosis. As tailored therapeutic approaches for the inhibition of endogenous MIF are being developed, it is important to evaluate the role of MIF in individual neoplastic conditions that may benefit from specific MIF inhibitors. Along with this line, in this paper, we have reviewed the evidence of the involvement of MIF in the etiopathogenesis and progression of glioblastoma and the preclinical data suggesting the possible use of specific MIF inhibition as a potential novel therapeutic strategy for brain tumors. PMID:29707160

Nuclear migration events throughout development

PubMed Central

Bone, Courtney R.

2016-01-01

ABSTRACT Moving the nucleus to a specific position within the cell is an important event during many cell and developmental processes. Several different molecular mechanisms exist to position nuclei in various cell types. In this Commentary, we review the recent progress made in elucidating mechanisms of nuclear migration in a variety of important developmental models. Genetic approaches to identify mutations that disrupt nuclear migration in yeast, filamentous fungi, Caenorhabditis elegans, Drosophila melanogaster and plants led to the identification of microtubule motors, as well as Sad1p, UNC-84 (SUN) domain and Klarsicht, ANC-1, Syne homology (KASH) domain proteins (LINC complex) that function to connect nuclei to the cytoskeleton. We focus on how these proteins and various mechanisms move nuclei during vertebrate development, including processes related to wound healing of fibroblasts, fertilization, developing myotubes and the developing central nervous system. We also describe how nuclear migration is involved in cells that migrate through constricted spaces. On the basis of these findings, it is becoming increasingly clear that defects in nuclear positioning are associated with human diseases, syndromes and disorders. PMID:27182060

Phytochemicals potently inhibit migration of metastatic breast cancer cells†

PubMed Central

Ham, Stephanie Lemmo; Nasrollahi, Samila; Shah, Kush N.; Soltisz, Andrew; Paruchuri, Sailaja; Yun, Yang H.; Luker, Gary D.; Bishayee, Anupam; Tavana, Hossein

2017-01-01

Cell migration is a major process that drives metastatic progression of cancers, the major cause of cancer death. Existing chemotherapeutic drugs have limited efficacy to prevent and/or treat metastasis, emphasizing the need for new treatments. We focus on triple negative breast cancer (TNBC), the subtype of breast cancer with worst prognosis and no standard chemotherapy protocols. Here we demonstrate that a group of natural compounds, known as phytochemicals, effectively block migration of metastatic TNBC cells. Using a novel cell micropatterning technology, we generate consistent migration niches in standard 96-well plates where each well contains a cell-excluded gap within a uniform monolayer of cells. Over time, cells migrate into and occupy the gap. Treating TNBC cells with non-toxic concentrations of phytochemicals significantly blocks motility of cells. Using a molecular analysis approach, we show that anti-migratory property of phytochemicals is partly due to their inhibitory effects on phosphorylation of ERK1/2. This study provides a framework for future studies to understand molecular targets of phytochemicals and evaluate their effectiveness in inhibiting metastasis in animal models of cancer. PMID:26120051

Utilization of design data on conventional system to building information modeling (BIM)

NASA Astrophysics Data System (ADS)

Akbar, Boyke M.; Z. R., Dewi Larasati

2017-11-01

Nowadays infrastructure development becomes one of the main priorities in the developed country such as Indonesia. The use of conventional design system is considered no longer effectively support the infrastructure projects, especially for the high complexity building design, due to its fragmented system issues. BIM comes as one of the solutions in managing projects in an integrated manner. Despite of the all known BIM benefits, there are some obstacles on the migration process to BIM. The two main of the obstacles are; the BIM implementation unpreparedness of some project parties and a concerns to leave behind the existing database and create a new one on the BIM system. This paper discusses the utilization probabilities of the existing CAD data from the conventional design system for BIM system. The existing conventional CAD data's and BIM design system output was studied to examine compatibility issues between two subject and followed by an utilization scheme-strategy probabilities. The goal of this study is to add project parties' eagerness in migrating to BIM by maximizing the existing data utilization and hopefully could also increase BIM based project workflow quality.

Minneapolis Multi-Ethnic Curriculum Project. Unit Overviews.

ERIC Educational Resources Information Center

Skjervold, Christian K.; Tipple, Bruce

The document presents unit overviews describing activities in the Minneapolis Multi-Ethnic Curriculum Project for secondary schools. It is divided into seven sections, each relating to a specific topic. Sections are entitled ethnicity, migration, acculturation, ethnic enclaves, family, prejudice/discrimination, and power. Each section offers from…

Projected changes of the low-latitude north-western Pacific wind-driven circulation under global warming

NASA Astrophysics Data System (ADS)

Duan, Jing; Chen, Zhaohui; Wu, Lixin

2017-05-01

Based on the outputs of 25 models participating in the Coupled Model Intercomparison Project Phase 5, the projected changes of the wind-driven circulation in the low-latitude north-western Pacific are evaluated. Results demonstrate that there will be a decrease in the mean transport of the North Equatorial Current (NEC), Mindanao Current, and Kuroshio Current in the east of the Philippines, accompanied by a northward shift of the NEC bifurcation Latitude (NBL) off the Philippine coast with over 30% increase in its seasonal south-north migration amplitude. Numerical simulations using a 1.5-layer nonlinear reduced-gravity ocean model show that the projected changes of the upper ocean circulation are predominantly determined by the robust weakening of the north-easterly trade winds and the associated wind stress curl under the El Niño-like warming pattern. The changes in the wind forcing and intensified upper ocean stratification are found equally important in amplifying the seasonal migration of the NBL.

Inflammation and cancer

PubMed Central

Coussens, Lisa M.; Werb, Zena

2009-01-01

Recent data have expanded the concept that inflammation is a critical component of tumour progression. Many cancers arise from sites of infection, chronic irritation and inflammation. It is now becoming clear that the tumour microenvironment, which is largely orchestrated by inflammatory cells, is an indispensable participant in the neoplastic process, fostering proliferation, survival and migration. In addition, tumour cells have co-opted some of the signalling molecules of the innate immune system, such as selectins, chemokines and their receptors for invasion, migration and metastasis. These insights are fostering new anti-inflammatory therapeutic approaches to cancer development. PMID:12490959

Emerging migration flows in a changing climate in dryland Africa

NASA Astrophysics Data System (ADS)

Kniveton, Dominic R.; Smith, Christopher D.; Black, Richard

2012-06-01

Fears of the movement of large numbers of people as a result of changes in the environment were first voiced in the 1980s (ref. ). Nearly thirty years later the numbers likely to migrate as a result of the impacts of climate change are still, at best, guesswork. Owing to the high prevalence of rainfed agriculture, many livelihoods in sub-Saharan African drylands are particularly vulnerable to changes in climate. One commonly adopted response strategy used by populations to deal with the resulting livelihood stress is migration. Here, we use an agent-based model developed around the theory of planned behaviour to explore how climate and demographic change, defined by the ENSEMBLES project and the United Nations Statistics Division of the Department of Economic and Social Affairs, combine to influence migration within and from Burkina Faso. The emergent migration patterns modelled support framing the nexus of climate change and migration as a complex adaptive system. Using this conceptual framework, we show that the extent of climate-change-related migration is likely to be highly nonlinear and the extent of this nonlinearity is dependent on population growth; therefore supporting migration policy interventions based on both demographic and climate change adaptation.

Nonlinear permanent migration response to climatic variations but minimal response to disasters

PubMed Central

Bohra-Mishra, Pratikshya; Oppenheimer, Michael; Hsiang, Solomon M.

2014-01-01

We present a microlevel study to simultaneously investigate the effects of variations in temperature and precipitation along with sudden natural disasters to infer their relative influence on migration that is likely permanent. The study is made possible by the availability of household panel data from Indonesia with an exceptional tracking rate combined with frequent occurrence of natural disasters and significant climatic variations, thus providing a quasi-experiment to examine the influence of environment on migration. Using data on 7,185 households followed over 15 y, we analyze whole-household, province-to-province migration, which allows us to understand the effects of environmental factors on permanent moves that may differ from temporary migration. The results suggest that permanent migration is influenced by climatic variations, whereas episodic disasters tend to have much smaller or no impact on such migration. In particular, temperature has a nonlinear effect on migration such that above 25 °C, a rise in temperature is related to an increase in outmigration, potentially through its impact on economic conditions. We use these results to estimate the impact of projected temperature increases on future permanent migration. Though precipitation also has a similar nonlinear effect on migration, the effect is smaller than that of temperature, underscoring the importance of using an expanded set of climatic factors as predictors of migration. These findings on the minimal influence of natural disasters and precipitation on permanent moves supplement previous findings on the significant role of these variables in promoting temporary migration. PMID:24958887

Nonlinear permanent migration response to climatic variations but minimal response to disasters.

PubMed

Bohra-Mishra, Pratikshya; Oppenheimer, Michael; Hsiang, Solomon M

2014-07-08

We present a microlevel study to simultaneously investigate the effects of variations in temperature and precipitation along with sudden natural disasters to infer their relative influence on migration that is likely permanent. The study is made possible by the availability of household panel data from Indonesia with an exceptional tracking rate combined with frequent occurrence of natural disasters and significant climatic variations, thus providing a quasi-experiment to examine the influence of environment on migration. Using data on 7,185 households followed over 15 y, we analyze whole-household, province-to-province migration, which allows us to understand the effects of environmental factors on permanent moves that may differ from temporary migration. The results suggest that permanent migration is influenced by climatic variations, whereas episodic disasters tend to have much smaller or no impact on such migration. In particular, temperature has a nonlinear effect on migration such that above 25 °C, a rise in temperature is related to an increase in outmigration, potentially through its impact on economic conditions. We use these results to estimate the impact of projected temperature increases on future permanent migration. Though precipitation also has a similar nonlinear effect on migration, the effect is smaller than that of temperature, underscoring the importance of using an expanded set of climatic factors as predictors of migration. These findings on the minimal influence of natural disasters and precipitation on permanent moves supplement previous findings on the significant role of these variables in promoting temporary migration.

Mexico: The Ultimate Domino?

DTIC Science & Technology

1988-05-01

five key issues confronting the two countries: foreign debt, trade, migration, illegal drugs, and foreign policy. The outlook for Mexican political ... stability is evaluated. The author concludes that Mexico’s financial condition presents near-term challenges, but its progress toward recovery, economic

Developing high-resolution spatial data of migration corridors for avian species of concern in regions of high potential wind development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Katzner, Todd

2014-06-15

The future of the US economy, our national security, and our environmental quality all depend on decreasing our reliance on foreign oil and on fossil fuels. An essential component of decreasing this reliance is the development of alternative energy sources. Wind power is among the most important alternative energy sources currently available, and the mid-Atlantic region is a primary focus for wind power development. In addition to being important to the development of wind power, the mid-Atlantic region holds a special responsibility for the conservation of the eastern North America's golden eagles (Aquila chrysaetos). This small population breeds in northeasternmore » Canada, winters in the southern Appalachians, and nearly all of these birds pass through the mid-Atlantic region twice each year. Movement of these birds is not random and, particularly during spring and autumn, migrating golden eagles concentrate in a narrow 30-50 mile wide corridor in central Pennsylvania. Thus, because the fate of these rare birds may depend on responsible management of the habitat they use it is critical to use research to identify ways to mitigate prospective impacts on this and similar raptor species. The goal of this project was to develop high-resolution spatial risk maps showing migration corridors of and habitat use by eastern golden eagles in regions of high potential for wind development. To accomplish this, we first expanded existing models of raptor migration for the eastern USA to identify broad-scale migration patterns. We then used data from novel high-resolution tracking devices to discover routes of passage and detailed flight behavior of individual golden eagles throughout the eastern USA. Finally, we integrated these data and models to predict population-level migration patterns and individual eagle flight behavior on migration. We then used this information to build spatially explicit, probabilistic maps showing relative risk to birds from wind development. This project has numerous benefits to people and to wildlife, primarily because it will provide a framework for safer and less controversial development of wind power. Because golden eagles are an important "umbrella" for other raptors, this project benefits a suite of species that may be impacted by wind turbines. Finally this work is a recognized priority for central Appalachian states and it is explicitly called for in, and meets the goals of, numerous state wildlife conservation plans. The final product we created, a region-wide map of relative risk to eagles of development of wind power, has allowed us to make specific recommendations regarding siting and operation of and mitigation at wind facilities. This approach also serves as a model for other projects to protect eagles in other places and to conserve suites of species beyond raptors.« less

United States History: From Community to Society. Unit Five: National Expansion. Grade Six. Project Social Studies.

ERIC Educational Resources Information Center

Minnesota Univ., Minneapolis. Project Social Studies Curriculum Center.

The purpose of this fifth in a series of resource units intended for use at the sixth grade level is to provide concepts on the internal migration of peoples from the Atlantic Seaboard and immigration of foreigners to the Northwest and New South. Students trace migration patterns, noting that culture and materials objects also move with the people…

Transparency in Distributed File Systems

DTIC Science & Technology

1989-01-01

ORGANIZATION NAME AND ADDRESS 10. PROGRAM ELEMENT. PROJECT, TASK Computer Science Department AREA & WORK UNIT NUMBERS 734 Comouter Studies Bldc . University of...sistency control , file and director) placement, and file and directory migration in a way that pro- 3 vides full network transparency. This transparency...areas of naming, replication, con- sistency control , file and directory placement, and file and directory migration in a way that pro- 3 vides full

Migration of the CERN IT Data Centre Support System to ServiceNow

NASA Astrophysics Data System (ADS)

Alvarez Alonso, R.; Arneodo, G.; Barring, O.; Bonfillou, E.; Coelho dos Santos, M.; Dore, V.; Lefebure, V.; Fedorko, I.; Grossir, A.; Hefferman, J.; Mendez Lorenzo, P.; Moller, M.; Pera Mira, O.; Salter, W.; Trevisani, F.; Toteva, Z.

2014-06-01

The large potential and flexibility of the ServiceNow infrastructure based on "best practises" methods is allowing the migration of some of the ticketing systems traditionally used for the monitoring of the servers and services available at the CERN IT Computer Centre. This migration enables the standardization and globalization of the ticketing and control systems implementing a generic system extensible to other departments and users. One of the activities of the Service Management project together with the Computing Facilities group has been the migration of the ITCM structure based on Remedy to ServiceNow within the context of one of the ITIL processes called Event Management. The experience gained during the first months of operation has been instrumental towards the migration to ServiceNow of other service monitoring systems and databases. The usage of this structure is also extended to the service tracking at the Wigner Centre in Budapest.

Border Enforcement and Return Migration by Documented and Undocumented Mexicans

PubMed Central

Massey, Douglas S.; Durand, Jorge; Pren, Karen A.

2014-01-01

Using data from the Mexican Migration Project we compute probabilities of departure and return for first and later trips to the United States in both documented and undocumented status. We then estimate statistical models to analyze the determinants of departure and return according to legal status. Prior to 1986, Mexico-U.S. migration was characterized by great circularity, but since then circularity has declined markedly for undocumented migrants but increased dramatically for documented migrants. Whereas return migration by undocumented migrants dropped in response to the massive increase in border enforcement, that of documented migrants did not. At present, the Mexico-U.S. migration system has reached a new equilibrium in which undocumented migrants are caged in as long term settlers in the United States while documented migrants increasingly range freely and circulate back and forth across the border within rising frequency. PMID:26273210

Migration, Business Formation, and the Informal Economy in Urban Mexico

PubMed Central

Riosmena, Fernando

2013-01-01

Although the informal economy has grown rapidly in several developing nations, and migration and informality may be related to similar types of credit constraints and market failures, previous research has not systematically attempted to identify if migrant households are more likely to start informal and formal businesses alike and if this association varies across local contexts. We examine the relationship between prior U.S. migration and the creation of both formal and informal businesses in urban Mexico using several criteria to indirectly assess sector location. We use data from 56 communities from the Mexican Migration Project to estimate multilevel survival and nonmultilevel competing risk models predicting the likelihood of informal, formal, and no business formation. The recent return migration of the household head is strongly associated with informal business creation, particularly in economically dynamic areas. On the other hand, migrants are only marginally more likely to start formal businesses in highly economically dynamic sending areas. PMID:23721676

Loss of Elp3 Impairs the Acetylation and Distribution of Connexin-43 in the Developing Cerebral Cortex

PubMed Central

Laguesse, Sophie; Close, Pierre; Van Hees, Laura; Chariot, Alain; Malgrange, Brigitte; Nguyen, Laurent

2017-01-01

The Elongator complex is required for proper development of the cerebral cortex. Interfering with its activity in vivo delays the migration of postmitotic projection neurons, at least through a defective α-tubulin acetylation. However, this complex is already expressed by cortical progenitors where it may regulate the early steps of migration by targeting additional proteins. Here we report that connexin-43 (Cx43), which is strongly expressed by cortical progenitors and whose depletion impairs projection neuron migration, requires Elongator expression for its proper acetylation. Indeed, we show that Cx43 acetylation is reduced in the cortex of Elp3cKO embryos, as well as in a neuroblastoma cell line depleted of Elp1 expression, suggesting that Cx43 acetylation requires Elongator in different cellular contexts. Moreover, we show that histones deacetylase 6 (HDAC6) is a deacetylase of Cx43. Finally, we report that acetylation of Cx43 regulates its membrane distribution in apical progenitors of the cerebral cortex. PMID:28507509

Movement between Mexico and Canada: Analysis of a New Migration Stream

PubMed Central

Massey, Douglas; Brown, Amelia E.

2011-01-01

In this analysis we use data from the Mexican Migration Project to contrast processes of Mexican migration to Canada and the United States. All migrants to Canada entered through the Seasonal Agricultural Worker Program and consistent with program criteria, migration there is strongly predicted by marital status and number of dependents, yielding a migrant population that is made up of males of prime labor-force age who are married and have multiple children at home. In contrast, the vast majority of migrants to the United States are undocumented and thus self-selected without regard to marital status or parenthood. Migration to the United States is strongly predicted by age, and migration probabilities display the age curve classically associated with labor migration. Within countries of destination, migrants to Canada enjoy superior labor market outcomes compared with those to the United States, with higher wages and more compact work schedules that yield higher earnings and shorter periods away from families compared with undocumented migrants to the United States. Labor migration to Canada also tends to operate as a circular flow with considerable repeat migration whereas undocumented migrants to the United States do not come and go so regularly, as crossing the Mexico-U.S. border has become increasingly difficult and costly. PMID:24347678

The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase

PubMed Central

Zhu, Zengrong; Bhat, Krishna Moorthi

2011-01-01

In the nervous system, neurons form in different regions, then they migrate and occupy specific positions. We have previously shown that RP2/sib, a well-studied neuronal pair in the Drosophila ventral nerve cord (VNC), has a complex migration route. Here, we show that the Hem protein, via the WAVE complex, regulates migration of GMC-1 and its progeny RP2 neuron. In Hem or WAVE mutants, RP2 neuron either abnormally migrates, crossing the midline from one hemisegment to the contralateral hemisegment, or does not migrate at al and fail to send out its axon projection. We report that Hem regulates neuronal migration through stabilizing WAVE. Since Hem and WAVE normally form a complex, our data argues that in the absence of Hem, WAVE, which is presumably no longer in a complex, becomes susceptible to degradation. We also find that Abelson Tyrosine kinase affects RP2 migration in a similar manner as Hem and WAVE, and appears to operate via WAVE. However, while Abl negatively regulates the levels of WAVE, it regulates migration via regulating the activity of WAVE. Our results also show that during the degradation of WAVE, Hem function is opposite to that of and downstream of Abl. PMID:21726548

Lincoln County nuclear waste project. Quarterly progress report, October 1, 1991--December 31, 1991

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-03-01

This document included the following three progress reports to the Yucca Mountain Project Office on radioactive waste storage in Lincoln County, Nevada: financial status report; federal cash transactions report; and technical progress report.

Lincoln County nuclear waste project. Quarterly progress report, January 1, 1992--March 31, 1992

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-03-01

This document included the following three progress reports to the Yucca Mountain Project Office on radioactive waste storage in Lincoln County, Nevada: financial status report; federal cash transactions report; and technical progress report.

Lincoln County nuclear waste project quarterly progress report, April 1, 1992--June 30, 1992

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-03-01

This document included the following three progress reports to the Yucca Mountain Project Office on radioactive waste storage in Lincoln County, Nevada: financial status report; federal cash transactions report; and technical progress report.

Time versus energy minimization migration strategy varies with body size and season in long-distance migratory shorebirds.

PubMed

Zhao, Meijuan; Christie, Maureen; Coleman, Jonathan; Hassell, Chris; Gosbell, Ken; Lisovski, Simeon; Minton, Clive; Klaassen, Marcel

2017-01-01

Migrants have been hypothesised to use different migration strategies between seasons: a time-minimization strategy during their pre-breeding migration towards the breeding grounds and an energy-minimization strategy during their post-breeding migration towards the wintering grounds. Besides season, we propose body size as a key factor in shaping migratory behaviour. Specifically, given that body size is expected to correlate negatively with maximum migration speed and that large birds tend to use more time to complete their annual life-history events (such as moult, breeding and migration), we hypothesise that large-sized species are time stressed all year round. Consequently, large birds are not only likely to adopt a time-minimization strategy during pre-breeding migration, but also during post-breeding migration, to guarantee a timely arrival at both the non-breeding (i.e. wintering) and breeding grounds. We tested this idea using individual tracks across six long-distance migratory shorebird species (family Scolopacidae) along the East Asian-Australasian Flyway varying in size from 50 g to 750 g lean body mass. Migration performance was compared between pre- and post-breeding migration using four quantifiable migratory behaviours that serve to distinguish between a time- and energy-minimization strategy, including migration speed, number of staging sites, total migration distance and step length from one site to the next. During pre- and post-breeding migration, the shorebirds generally covered similar distances, but they tended to migrate faster, used fewer staging sites, and tended to use longer step lengths during pre-breeding migration. These seasonal differences are consistent with the prediction that a time-minimization strategy is used during pre-breeding migration, whereas an energy-minimization strategy is used during post-breeding migration. However, there was also a tendency for the seasonal difference in migration speed to progressively disappear with an increase in body size, supporting our hypothesis that larger species tend to use time-minimization strategies during both pre- and post-breeding migration. Our study highlights that body size plays an important role in shaping migratory behaviour. Larger migratory bird species are potentially time constrained during not only the pre- but also the post-breeding migration. Conservation of their habitats during both seasons may thus be crucial for averting further population declines.

HARVARD PROJECT PHYSICS PROGRESS REPORT.

ERIC Educational Resources Information Center

Harvard Univ., Cambridge, MA.

THIS REPORT OF HARVARD PROJECT PHYSICS PRESENTS DRAFTS OF THREE SPEECHES DELIVERED TO THE AMERICAN ASSOCIATION OF PHYSICS TEACHERS (AAPT) MEETING, FEBRUARY, 1967. THE ADDRESS BY GERALD HOLTON DEALS WITH THE AIMS AND PROGRESS OF THE PROJECT. DISCUSSED ARE (1) PROJECT PARTICIPANTS, (2) AIMS AND CONTENT, (3) THE NEW EMPHASIS, (4) SURVEY OF COURSE…

Cadherin-11 Mediates Contact Inhibition of Locomotion during Xenopus Neural Crest Cell Migration

PubMed Central

Becker, Sarah F. S.; Mayor, Roberto; Kashef, Jubin

2013-01-01

Collective cell migration is an essential feature both in embryonic development and cancer progression. The molecular mechanisms of these coordinated directional cell movements still need to be elucidated. The migration of cranial neural crest (CNC) cells during embryogenesis is an excellent model for collective cell migration in vivo. These highly motile and multipotent cells migrate directionally on defined routes throughout the embryo. Interestingly, local cell-cell interactions seem to be the key force for directionality. CNC cells can change their migration direction by a repulsive cell response called contact inhibition of locomotion (CIL). Cell protrusions collapse upon homotypic cell-cell contact and internal repolarization leads to formation of new protrusions toward cell-free regions. Wnt/PCP signaling was shown to mediate activation of small RhoGTPase RhoA and inhibition of cell protrusions at the contact side. However, the mechanism how a cell recognizes the contact is poorly understood. Here, we demonstrate that Xenopus cadherin-11 (Xcad-11) mediated cell-cell adhesion is necessary in CIL for directional and collective migration of CNC cells. Reduction of Xcad-11 adhesive function resulted in higher invasiveness of CNC due to loss of CIL. Additionally, transplantation analyses revealed that CNC migratory behaviour in vivo is non-directional and incomplete when Xcad-11 adhesive function is impaired. Blocking Wnt/PCP signaling led to similar results underlining the importance of Xcad-11 in the mechanism of CIL and directional migration of CNC. PMID:24392028

Interaction between migration behaviour and estuarine mortality in cultivated Atlantic salmon Salmo salar smolts.

PubMed

Vollset, K W; Mahlum, S; Davidsen, J G; Skoglund, H; Barlaup, B T

2016-10-01

Migration behaviour and estuarine mortality of cultivated Atlantic salmon Salmo salar smolts in a 16 km long estuary were studied using two methods: (1) acoustic telemetry and (2) group tagging in combination with trap nets. Progression rates of surviving individuals through the estuary were relatively slow using both methods [0·38 L T (total length) s -1 v. 0·25 L T  s -1 ]. In 2012, the progression rate was slow from the river to the estuary (0·55 L T  s -1 ) and the first part of the estuary (0·31 L T  s -1 ), but increased thereafter (1·45-2·21 L T  s -1 ). In 2013, the progression rate was fast from the river to the estuary (4·31 L T  s -1 ) but was slower thereafter (0·18-0·91 L T  s -1 ). Survival to the fjord was higher in 2012 (47%) compared to 2013 (6%). Fast moving individuals were more likely to migrate successfully through the estuary compared to slower moving individuals. Adult recapture of coded-wire-tagged S. salar was generally low (0·00-0·04%). Mortality hot spots were related to topographically distinct areas such as the river outlet (in 2012) or the sill separating the estuary and the fjord (in 2013). At the sill, an aggregation of cod Gadus morhua predating on cultivated smolts was identified. The results indicate that slow progression rates through the estuary decreases the likelihood of smolts being detected outside the estuary. The highly stochastic and site-specific mortality patterns observed in this study highlight the complexity in extrapolating mortality patterns of single release groups to the entire smolt run of wild S. salar. © 2016 The Fisheries Society of the British Isles.

Overexpression of EMMPRIN Isoform 2 Is Associated with Head and Neck Cancer Metastasis

PubMed Central

Guo, Weijie; Wang, Lili; Li, Haigang; Zhang, Tianyu; Liu, Xiaojia; Xu, Qin; Li, Jinsong; Guo, Zhongmin

2014-01-01

Extracellular matrix metalloproteinase inducer (EMMPRIN), a plasma membrane protein of the immunoglobulin (Ig) superfamily, has been reported to promote cancer cell invasion and metastasis in several human malignancies. However, the roles of the different EMMPRIN isoforms and their associated mechanisms in head and neck cancer progression remain unknown. Using quantitative real-time PCR, we found that EMMPRIN isoform 2 (EMMPRIN-2) was the only isoform that was overexpressed in both head and neck cancer tissues and cell lines and that it was associated with head and neck cancer metastasis. To determine the effects of EMMPRIN-2 on head and neck cancer progression, we transfected head and neck cancer cells with an EMMPRIN-2 expression vector and EMMPRIN-2 siRNA to exogenously modulate EMMPRIN-2 expression and examined the functional importance of EMMPRIN-2 in head and neck cancer invasion and metastasis. We found that EMMPRIN-2 promoted head and neck cancer cell invasion, migration, and adhesion in vitro and increased lung metastasis in vivo. Mechanistic studies revealed that EMMPRIN-2 overexpression promoted the secretion of extracellular signaling molecules, including matrix metalloproteinases-2(MMP-2), urokinase-type plasminogen activator(uPA) and Cathepsin B, in head and neck cancer cells. While MMP-2 and uPA have been demonstrated to be important mediators of EMMPRIN signaling, the role of Cathepsin B in EMMPRIN-mediated molecular cascades and tumorigenesis has not been established. We found that EMMPRIN-2 overexpression and Cathepsin B down-regulation significantly inhibited the invasion, migration and adhesion of Tca8133 cells, suggesting that Cathepsin B is required for EMMPRIN-2 enhanced cell migration and invasion in head and neck cancer. The results of our study demonstrate the important role of EMMPRIN-2 in head and neck cancer progression for the first time and reveal that increased extracellular secretion of Cathepsin B may be a novel mechanism underlying EMMPRIN-2 enhanced tumor progression in head and neck cancer. PMID:24705283

Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis.

PubMed

Huang, Zhiquan; Tan, Ning; Guo, Weijie; Wang, Lili; Li, Haigang; Zhang, Tianyu; Liu, Xiaojia; Xu, Qin; Li, Jinsong; Guo, Zhongmin

2014-01-01

Extracellular matrix metalloproteinase inducer (EMMPRIN), a plasma membrane protein of the immunoglobulin (Ig) superfamily, has been reported to promote cancer cell invasion and metastasis in several human malignancies. However, the roles of the different EMMPRIN isoforms and their associated mechanisms in head and neck cancer progression remain unknown. Using quantitative real-time PCR, we found that EMMPRIN isoform 2 (EMMPRIN-2) was the only isoform that was overexpressed in both head and neck cancer tissues and cell lines and that it was associated with head and neck cancer metastasis. To determine the effects of EMMPRIN-2 on head and neck cancer progression, we transfected head and neck cancer cells with an EMMPRIN-2 expression vector and EMMPRIN-2 siRNA to exogenously modulate EMMPRIN-2 expression and examined the functional importance of EMMPRIN-2 in head and neck cancer invasion and metastasis. We found that EMMPRIN-2 promoted head and neck cancer cell invasion, migration, and adhesion in vitro and increased lung metastasis in vivo. Mechanistic studies revealed that EMMPRIN-2 overexpression promoted the secretion of extracellular signaling molecules, including matrix metalloproteinases-2(MMP-2), urokinase-type plasminogen activator(uPA) and Cathepsin B, in head and neck cancer cells. While MMP-2 and uPA have been demonstrated to be important mediators of EMMPRIN signaling, the role of Cathepsin B in EMMPRIN-mediated molecular cascades and tumorigenesis has not been established. We found that EMMPRIN-2 overexpression and Cathepsin B down-regulation significantly inhibited the invasion, migration and adhesion of Tca8133 cells, suggesting that Cathepsin B is required for EMMPRIN-2 enhanced cell migration and invasion in head and neck cancer. The results of our study demonstrate the important role of EMMPRIN-2 in head and neck cancer progression for the first time and reveal that increased extracellular secretion of Cathepsin B may be a novel mechanism underlying EMMPRIN-2 enhanced tumor progression in head and neck cancer.

Heparanase induced by advanced glycation end products (AGEs) promotes macrophage migration involving RAGE and PI3K/AKT pathway

PubMed Central

2013-01-01

Background Advanced glycation end products (AGEs), inflammatory-associated macrophage migration and accumulation are crucial for initiation and progression of diabetic vascular complication. Enzymatic activity of heparanase (HPA) is implicated strongly in dissemination of metastatic tumor cells and cells of the immune system. In addition, HPA enhances the phosphorylation of selected signaling molecules including AKT pathway independent of enzymatic activity. However, virtually nothing is presently known the role of HPA during macrophage migration exposed to AGEs involving signal pathway. Methods These studies were carried out in Ana-1 macrophages. Macrophage viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. HPA and AKT protein expression in macrophages are analysed by Western blotting and HPA mRNA expression by real time quantitative RT-PCR. Release of HPA was determined by ELISA. Macrophage migration was assessed by Transwell assays. Results HPA protein and mRNA were found to be increased significantly in AGEs-treated macrophages. Pretreatment with anti-HPA antibody which recognizes the nonenzymatic terminal of HPA prevented AGEs-induced AKT phosphorylation and macrophage migration. LY294002 (PI3k/AKT inhibitor) inhibited AGEs-induced macrophage migration. Furthermore, pretreatment with anti-receptor for advanced glycation end products (RAGE) antibody attenuated AGEs-induced HPA expression, AKT phosphorylation and macrophage migration. Conclusions These data indicate that AGEs-induced macrophage migration is dependent on HPA involving RAGE-HPA-PI3K/AKT pathway. The nonenzymatic activity of HPA may play a key role in AGEs-induced macrophage migration associated with inflammation in diabetic vascular complication. PMID:23442498

Amplification or suppression: Social networks and the climate change-migration association in rural Mexico.

PubMed

Nawrotzki, Raphael J; Riosmena, Fernando; Hunter, Lori M; Runfola, Daniel M

2015-11-01

Increasing rates of climate migration may be of economic and national concern to sending and destination countries. It has been argued that social networks - the ties connecting an origin and destination - may operate as "migration corridors" with the potential to strongly facilitate climate change-related migration. This study investigates whether social networks at the household and community levels amplify or suppress the impact of climate change on international migration from rural Mexico. A novel set of 15 climate change indices was generated based on daily temperature and precipitation data for 214 weather stations across Mexico. Employing geostatistical interpolation techniques, the climate change values were linked to 68 rural municipalities for which sociodemographic data and detailed migration histories were available from the Mexican Migration Project. Multi-level discrete-time event-history models were used to investigate the effect of climate change on international migration between 1986 and 1999. At the household level, the effect of social networks was approximated by comparing the first to the last move, assuming that through the first move a household establishes internal social capital. At the community level, the impact of social capital was explored through interactions with a measure of the proportion of adults with migration experience. The results show that rather than amplifying , social capital may suppress the sensitivity of migration to climate triggers, suggesting that social networks could facilitate climate change adaptation in place.

Adenosine Stimulate Proliferation and Migration in Triple Negative Breast Cancer Cells

PubMed Central

Fernandez-Gallardo, Miriam; González-Ramírez, Ricardo; Sandoval, Alejandro; Monjaraz, Eduardo

2016-01-01

Emerging evidence suggests that the adenosine (Ado) receptors may play crucial roles in tumor progression. Here, we show that Ado increases proliferation and migration in a triple negative breast cancer model, the MDA-MB 231 cell line. The use of specific agonists and antagonists evidenced that these effects depend on the activation of the A2B receptor, which then triggers an intracellular response mediated by the adenylate cyclase/PKA/cAMP signaling pathway. Ado also increases the expression of NaV1.5 channels, a potential biomarker in breast cancer. Together, these data suggest important roles of the A2B receptors and NaV1.5 channels in the Ado-induced increase in proliferation and migration of the MDA-MB 231 cells. PMID:27911956

IMGui-A Desktop GUI Application for Isolation with Migration Analyses.

PubMed

Knoblauch, Jared; Sethuraman, Arun; Hey, Jody

2017-02-01

The Isolation with Migration (IM) programs (e.g., IMa2) have been utilized extensively by evolutionary biologists for model-based inference of demographic parameters including effective population sizes, migration rates, and divergence times. Here, we describe a graphical user interface for the latest IM program. IMGui provides a comprehensive set of tools for performing demographic analyses, tracking progress of runs, and visualizing results. Developed using node. js and the Electron framework, IMGui is an application that runs on any desktop operating system, and is available for download at https://github.com/jaredgk/IMgui-electron-packages. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Managed Migration: The Caribbean Approach to Addressing Nursing Services Capacity

PubMed Central

Salmon, Marla E; Yan, Jean; Hewitt, Hermi; Guisinger, Victoria

2007-01-01

Objective To (1) provide a contextual analysis of the Caribbean region with respect to forces shaping the current and emerging nursing workforce picture in the region; (2) discuss country-specific case(s) within the Caribbean; and (3) describe the Managed Migration Program as a potential framework for addressing regional and global nurse migration issues. Principal Findings The Caribbean is in the midst of a crisis of shortages of nurses with an average vacancy rate of 42 percent. Low pay, poor career prospects, and lack of education opportunities are among the reasons nurses resign. Many of these nurses look outside the region for job opportunities in the United Kingdom, Canada, the United States, and other countries. Compounding the situation is the lack of resources to train nurses to fill the vacancies. The Managed Migration Program of the Caribbean is a multilateral, cross-sector, multi-interventional, long-term strategy for developing and maintaining an adequate supply of nurses for the region. Conclusions The Managed Migration Program of the Caribbean has made progress in establishing regional support for addressing the nursing shortage crisis and developing a number of interesting initiatives such as training for export and temporary migration. Recommendations to move the Managed Migration Program of the Caribbean forward focus on advocacy, integration of the program into regional policy decisions, and integration of the program with regional health programming. PMID:17489919

Increased dermal collagen bundle alignment in systemic sclerosis is associated with a cell migration signature and role of Arhgdib in directed fibroblast migration on aligned ECMs

PubMed Central

Lafyatis, Robert; Burkly, Linda C.

2017-01-01

Systemic sclerosis (SSc) is a devastating disease affecting the skin and internal organs. Dermal fibrosis manifests early and Modified Rodnan Skin Scores (MRSS) correlate with disease progression. Transcriptomics of SSc skin biopsies suggest the role of the in vivo microenvironment in maintaining the pathological myofibroblasts. Therefore, defining the structural changes in dermal collagen in SSc patients could inform our understanding of fibrosis pathogenesis. Here, we report a method for quantitative whole-slide image analysis of dermal collagen from SSc patients, and our findings of more aligned dermal collagen bundles in diffuse cutaneous SSc (dcSSc) patients. Using the bleomycin-induced mouse model of SSc, we identified a distinct high dermal collagen bundle alignment gene signature, characterized by a concerted upregulation in cell migration, adhesion, and guidance pathways, and downregulation of spindle, replication, and cytokinesis pathways. Furthermore, increased bundle alignment induced a cell migration gene signature in fibroblasts in vitro, and these cells demonstrated increased directed migration on aligned ECM fibers that is dependent on expression of Arhgdib (Rho GDP-dissociation inhibitor 2). Our results indicate that increased cell migration is a cellular response to the increased collagen bundle alignment featured in fibrotic skin. Moreover, many of the cell migration genes identified in our study are shared with human SSc skin and may be new targets for therapeutic intervention. PMID:28662216

Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF

PubMed Central

Liu, Yang; Liu, Yuanyuan; Sun, Chao; Gan, Lu; Zhang, Luwei; Mao, Aihong; Du, Yuting; Zhou, Rong; Zhang, Hong

2014-01-01

This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditioned medium from cultures of glioma cells irradiated at a range of doses and the migration of both cell types, tube formation by endothelial cells, as well as the expression and secretion of migration-related proteins were evaluated. Exposure to X-ray radiation-conditioned medium induced dose-dependent increases in cell migration and tube formation, which were accompanied by an upregulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and -9 expression. However, glioma cells treated with conditioned medium of cells irradiated at a carbon ion dose of 4.0 Gy showed a marked decrease in migratory potential and VEGF secretion relative to non-irradiated cells. The application of recombinant VEGF165 stimulated migration in glioma and endothelial cells, which was associated with increased FAK phosphorylation at Tyr861, suggesting that the suppression of cell migration by carbon ion radiation could be via VEGF-activated FAK signaling. Taken together, these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment. PMID:24893038

Progesterone stimulates the long-distance migration of capacitated ram spermatozoa through viscous media under geotactic condition.

PubMed

Martínez-Rodríguez, Carmen; Anel-López, Luis; Alvarez, Mercedes; Ortega-Ferrusola, Cristina; Boixo, Juan Carlos; Peña, Fernando J; Anel, Luis; de Paz, Paulino

2018-05-15

Forward progressive motility of spermatozoa is an essential prerequisite for reproductive success, and sperm navigation is assisted by guidance mechanisms that may depend on micro-environmental factors. In the present study, we performed an integrated analysis of long-distance ram sperm migration in vitro that combined two environmental factors (10 μM progesterone and a geotactic effect) and the physiological status of the cells (capacitation treatment). A penetration assay was used in which spermatozoa had to travel 20 mm in a viscous medium (two media of differing viscosity: acrylamide and hyaluronic acid) through a tube device. The number of migrating spermatozoa, the physiology of the cells (motility analyzed using a CASA system; acrosomal status, viability and active mitochondria evaluated by flow cytometry; DNA fragmentation index calculated by quantitative PCR) and the morphometry of sperm heads (performed using an image analysis system) were evaluated after long-distance sperm migration. Ram sperm capacitation significantly stimulates cell migration through viscous media under geotactic conditions, and this effect is enhanced by progesterone induction. The rheological characteristics of viscous media have a marked impact on ram sperm migration, and acrylamide more favorably facilitates navigation over a large distance. The migrating spermatozoa are morphologically better adapted (high ellipticity) for displacement in viscous media and exhibit remarkably depleted mitochondrial membrane potential. Copyright © 2018 Elsevier Inc. All rights reserved.

Tornadic storm avoidance behavior in breeding songbirds

USGS Publications Warehouse

Streby, Henry M.; Kramer, Gunnar R.; Peterson, Sean M.; Lehman, Justin A.; Buehler, David A.; Andersen, David E.

2015-01-01

Migration is a common behavior used by animals of many taxa to occupy different habitats during different periods. Migrant birds are categorized as either facultative (i.e., those that are forced to migrate by some proximal cue, often weather) or obligate (i.e., those that migrate on a regular cycle). During migration, obligate migrants can curtail or delay flights in response to inclement weather or until favorable winds prevail, and they can temporarily reorient or reverse direction when ecological or meteorological obstacles are encountered. However, it is not known whether obligate migrants undertake facultative migrations and make large-scale movements in response to proximal cues outside of their regular migration periods. Here, we present the first documentation of obligate long-distance migrant birds undertaking a facultative migration, wherein breeding golden-winged warblers (Vermivora chrysoptera) carrying light-level geolocators performed a >1,500 km 5-day circumvention of a severe tornadic storm. The birds evacuated their breeding territories >24 hr before the arrival of the storm and atmospheric variation associated with it. The probable cue, radiating >1,000 km from tornadic storms, perceived by birds and influencing bird behavior and movements, is infrasound (i.e., sound below the range of human hearing). With the predicted increase in severity and frequency of similar storms as anthropogenic climate change progresses, understanding large-scale behavioral responses of animals to such events will be an important objective of future research.

Regulation of tumor progression via the Snail-RKIP signaling pathway by nicotine exposure in head and neck squamous cell carcinoma.

PubMed

Nieh, Shin; Jao, Shu-Wen; Yang, Chin-Yuh; Lin, Yaoh-Shiang; Tseng, Yi-Han; Liu, Chia-Lin; Lee, Tsai-Yu; Liu, Tsung-Yun; Chu, Yueng-Hsiang; Chen, Su-Feng

2015-12-01

Recent studies suggest that long-term exposure of the carcinogen 4-methylnitrosamino-1-3-pyridyl-1-butanone (NNK) found in tobacco smoke is involved in the progression of head and neck squamous cell carcinoma (HNSCC). The underlying nicotine-mediated mechanism remains unclear. An analysis of SCC-25 and Fadu cells with or without NNK exposure focusing on the evaluation of migration and invasion abilities, the expression of epithelial-mesenchymal transition, drug-resistance-related genes, properties of cancer stem cells (CSCs), and anti-apoptosis was performed. Long-term NNK exposure enhances migration and invasion with morphological alterations in a dose-dependently manner. Furthermore, NNK exposure also upregulates Snail, promotes sphere-forming ability, and overexpresses aldehyde dehydrogenase 1 (ALDH1), Nanog, OCT4, ABCG2, and MDR1. The current study confirmed that long-term NNK exposure plays a role in HNSCC by increasing anti-apoptosis and therapeutic resistance via the Snail-RKIP signaling pathway. Our data also suggest that α7 nicotinic acetylcholine receptor (α7-nAChR) inhibition or targeting Snail may provide a feasible rationale for preventing the progression of HNSCC. © 2015 Wiley Periodicals, Inc.

Circular RNA hsa_circ_0008039 promotes breast cancer cell proliferation and migration by regulating miR-432-5p/E2F3 axis.

PubMed

Liu, Yanhua; Lu, Cuntao; Zhou, Yizhou; Zhang, Zhihong; Sun, Li

2018-07-20

As the development of sequencing technology, more and more circular RNAs (circRNAs) are identified in human cancer tissues. Increasing evidences imply circRNAs are important regulators in tumor progression. Nevertheless, how circRNAs participate in breast cancer development and progression is not well understood. In the present study, we identified a novel circRNA hsa_circ_0008039 with upregulated expression level in breast cancer tissues. By functional experiments, we found that hsa_circ_0008039 depletion significantly suppressed the proliferation, arrested cell-cycle progression and reduced migration in breast cancer. Mechanistic investigations suggested that hsa_circ_0008039 served as a competing endogenous RNA (ceRNA) of miR-432-5p. Subsequently, E2F3 was identified as the functional target of miR-432-5p and overexpression of hsa_circ_0008039 elevated E2F3 expression in breast cancer. On the whole, our study indicated that hsa_circ_0008039 exerted oncogenic roles in breast cancer and suggested the hsa_circ_0008039/miR-432-5p/E2F3 axis might be a potential therapeutic target. Copyright © 2018 Elsevier Inc. All rights reserved.

Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma.

PubMed

Zhang, Dawei; Li, Haiyan; Xie, Juping; Jiang, Decan; Cao, Liangqi; Yang, Xuewei; Xue, Ping; Jiang, Xiaofeng

2018-06-01

The aim of the present study was to elucidate whether, and how, long intergenic non-protein coding RNA 1296 (LINC01296) is involved in the modulation of human cholangiocarcinoma (CCA) development and progression. Microarray data analysis and reverse transcription-quantitative polymerase chain reaction analysis demonstrated that LINC01296 was significantly upregulated in human CCA compared with nontumor tissues. Furthermore, the expression of LINC01296 in human CCA was positively associated with tumor severity and clinical stage. Knockdown of LINC01296 dramatically suppressed the viability, migration and invasion of RBE and CCLP1 cells, and promoted cell apoptosis in vitro. Furthermore, LINC01296 knockdown inhibited tumor growth in a xenograft model. Mechanistically, LINC01296 was demonstrated to sponge microRNA-5095 (miR-5095), which targets MYCN proto-oncogene bHLH transcription factor (MYCN) mRNA in human CCA. By inhibition of miR-5095, LINC01296 overexpression upregulated the expression of MYCN and promoted cell viability, migration and invasion in CCA cells. The results reveal that the axis of LINC01296/miR-5095/MYCN may be a mechanism to regulate CCA development and progression.

Novel insights into a retinoic-acid-induced cleft palate based on Rac1 regulation of the fibronectin arrangement.

PubMed

Tang, Qinghuang; Li, Liwen; Lee, Min-Jung; Ge, Qing; Lee, Jong-Min; Jung, Han-Sung

2016-03-01

Retinoic acid (RA)-induced cleft palate results from both extrinsic obstructions by the tongue and internal factors within the palatal shelves. Our previous study showed that the spatiotemporal expression of Rac1 regulates the fibronectin (FN) arrangement through cell density alterations that play an important role in palate development. In this study, we investigate the involvement of the Rac1 regulation of the FN arrangement in RA-induced cleft palate. Our results demonstrate that RA-induced intrinsic alterations in palatal shelves, including a delayed progress of cell condensation, delay palate development, even after the removal of the tongue. Further analysis shows that RA treatment diminishes the region-distinctive expression of Rac1 within the palatal shelves, which reversely alters the fibrillar arrangement of FN. Furthermore, RA treatment disrupts the formation of lamellipodia, which are indicative structures of cell migration that are regulated by Rac1. These results suggest that the Rac1 regulation of the FN arrangement is involved in RA-induced cleft palate through the regulation of cell migration, which delays the progress of cell condensation and subsequently influences the FN arrangement, inducing a delay in palate development. Our study provides new insights into the RA-induced impairment of palatal shelf elevation based on cell migration dynamics.

Pleiotrophin Exerts Its Migration and Invasion Effect through the Neuropilin-1 Pathway

PubMed Central

Elahouel, Rania; Blanc, Charly; Carpentier, Gilles; Frechault, Sophie; Cascone, Ilaria; Destouches, Damien; Delbé, Jean; Courty, José; Hamma-Kourbali, Yamina

2015-01-01

Pleiotrophin (PTN) is a pleiotropic growth factor that exhibits angiogenic properties and is involved in tumor growth and metastasis. Although it has been shown that PTN is expressed in tumor cells, few studies have investigated its receptors and their involvement in cell migration and invasion. Neuropilin-1 (NRP-1) is a receptor for multiple growth factors that mediates cell motility and plays an important role in angiogenesis and tumor progression. Here we provide evidence for the first time that NRP-1 is crucial for biological activities of PTN. We found that PTN interacted directly with NRP-1 through its thrombospondin type-I repeat domains. Importantly, binding of PTN to NRP-1 stimulated the internalization and recycling of NRP-1 at the cell surface. Invalidation of NRP-1 by RNA interference in human carcinoma cells inhibited PTN-induced intracellular signaling of the serine-threonine kinase, mitogen-activated protein MAP kinase, and focal adhesion kinase pathways. Accordingly, NRP-1 silencing or blocking by antibody inhibited PTN-induced human umbilical vein endothelial cell migration and tumor cell invasion. These results suggest that NRP-1/PTN interaction provides a novel mechanism for controlling the response of endothelial and tumoral cells to PTN and may explain, at least in part, how PTN contributes to tumor angiogenesis and cancer progression. PMID:26408254

Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.

PubMed

Bello, Sanusi M; Millo, Hadas; Rajebhosale, Manisha; Price, Stephen R

2012-01-11

Motor neurons that control limb movements are organized as a neuronal nucleus in the developing ventral horn of the spinal cord called the lateral motor column. Neuronal migration segregates motor neurons into distinct lateral and medial divisions within the lateral motor column that project axons to dorsal or ventral limb targets, respectively. This migratory phase is followed by an aggregation phase whereby motor neurons within a division that project to the same muscle cluster together. These later phases of motor neuron organization depend on limb-regulated differential cadherin expression within motor neurons. Initially, all motor neurons display the same cadherin expression profile, which coincides with the migratory phase of motor neuron segregation. Here, we show that this early, pan-motor neuron cadherin function drives the divisional segregation of spinal motor neurons in the chicken embryo by controlling motor neuron migration. We manipulated pan-motor neuron cadherin function through dissociation of cadherin binding to their intracellular partners. We found that of the major intracellular transducers of cadherin signaling, γ-catenin and α-catenin predominate in the lateral motor column. In vivo manipulations that uncouple cadherin-catenin binding disrupt divisional segregation via deficits in motor neuron migration. Additionally, reduction of the expression of cadherin-7, a cadherin predominantly expressed in motor neurons only during their migration, also perturbs divisional segregation. Our results show that γ-catenin-dependent cadherin function is required for spinal motor neuron migration and divisional segregation and suggest a prolonged role for cadherin expression in all phases of motor neuron organization.

Model study of St. Stephen powerhouse fish passage facilities, Cooper River rediversion project, South Carolina. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hite, J.E.; Murphy, T.E.

1998-09-01

This report documents a model study of the St. Stephen Power Plant, located in Berkely County, South Carolina. A previous model study revealed that the fish lift at the powerhouse could be improved by providing auxiliary attraction flows to the fish entrances. An auxiliary attraction flow (AAF) system was proposed that uses a siphon to obtain the auxiliary attraction water from the reservoir. The model investigations reported herein address the flow conditions at the discharge end of the siphon; the hydraulic aspects of the siphon are not addressed. Three different models were used to evaluate flow conditions at the dischargemore » end of the AAF system. A 1:25-scale model of the St. Stephen powerhouse was used to improve the fish entrance conditions and to evaluate the outlet conditions for the initial AAF system. As the investigations progressed, the design of the siphon discharge system was modified to include downstream fish migration and debris passage.« less

Global warming and extinctions of endemic species from biodiversity hotspots.

PubMed

Malcolm, Jay R; Liu, Canran; Neilson, Ronald P; Hansen, Lara; Hannah, Lee

2006-04-01

Global warming is a key threat to biodiversity, but few researchers have assessed the magnitude of this threat at the global scale. We used major vegetation types (biomes) as proxies for natural habitats and, based on projected future biome distributions under doubled-CO2 climates, calculated changes in habitat areas and associated extinctions of endemic plant and vertebrate species in biodiversity hotspots. Because of numerous uncertainties in this approach, we undertook a sensitivity analysis of multiple factors that included (1) two global vegetation models, (2) different numbers of biome classes in our biome classification schemes, (3) different assumptions about whether species distributions were biome specific or not, and (4) different migration capabilities. Extinctions were calculated using both species-area and endemic-area relationships. In addition, average required migration rates were calculated for each hotspot assuming a doubled-CO2 climate in 100 years. Projected percent extinctions ranged from <1 to 43% of the endemic biota (average 11.6%), with biome specificity having the greatest influence on the estimates, followed by the global vegetation model and then by migration and biome classification assumptions. Bootstrap comparisons indicated that effects on hotpots as a group were not significantly different from effects on random same-biome collections of grid cells with respect to biome change or migration rates; in some scenarios, however, botspots exhibited relatively high biome change and low migration rates. Especially vulnerable hotspots were the Cape Floristic Region, Caribbean, Indo-Burma, Mediterranean Basin, Southwest Australia, and Tropical Andes, where plant extinctions per hotspot sometimes exceeded 2000 species. Under the assumption that projected habitat changes were attained in 100 years, estimated global-warming-induced rates of species extinctions in tropical hotspots in some cases exceeded those due to deforestation, supporting suggestions that global warming is one of the most serious threats to the planet's biodiversity.

FAM13A is associated with non-small cell lung cancer (NSCLC) progression and controls tumor cell proliferation and survival

PubMed Central

Heim, Lisanne; Trump, Sonja; Mittler, Susanne; Sopel, Nina; Andreev, Katerina; Ferrazzi, Fulvia; Ekici, Arif B.; Rieker, Ralf; Springel, Rebekka; Assmann, Vera L.; Lechmann, Matthias; Koch, Sonja; Engelhardt, Marina; Trufa, Denis I.; Sirbu, Horia; Hartmann, Arndt; Finotto, Susetta

2017-01-01

ABSTRACT Genome-wide association studies (GWAS) associated Family with sequence similarity 13, member A (FAM13A) with non-small cell lung cancer (NSCLC) occurrence. Here, we found increased numbers of FAM13A protein expressing cells in the tumoral region of lung tissues from a cohort of patients with NSCLC. Moreover, FAM13A inversely correlated with CTLA4 but directly correlated with HIF1α levels in the control region of these patients. Consistently, FAM13A RhoGAP was found to be associated with T cell effector molecules like HIF1α and Tbet and was downregulated in immunosuppressive CD4+CD25+Foxp3+CTLA4+ T cells. TGFβ, a tumor suppressor factor, as well as siRNA to FAM13A, suppressed both isoforms of FAM13A and inhibited tumor cell proliferation. RNA-Seq analysis confirmed this finding. Moreover, siRNA to FAM13A induced TGFβ levels. Finally, in experimental tumor cell migration, FAM13A was induced and TGFβ accelerated this process by inducing cell migration, HIF1α, and the FAM13A RhoGAP isoform. Furthermore, siRNA to FAM13A inhibited tumor cell proliferation and induced cell migration without affecting HIF1α. In conclusion, FAM13A is involved in tumor cell proliferation and downstream of TGFβ and HIF1α, FAM13A RhoGAP is associated with Th1 gene expression and lung tumor cell migration. These findings identify FAM13A as key regulator of NSCLC growth and progression. PMID:28197372

Trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses.

PubMed

Twu, Olivia; Dessí, Daniele; Vu, Anh; Mercer, Frances; Stevens, Grant C; de Miguel, Natalia; Rappelli, Paola; Cocco, Anna Rita; Clubb, Robert T; Fiori, Pier Luigi; Johnson, Patricia J

2014-06-03

The human-infective parasite Trichomonas vaginalis causes the most prevalent nonviral sexually transmitted infection worldwide. Infections in men may result in colonization of the prostate and are correlated with increased risk of aggressive prostate cancer. We have found that T. vaginalis secretes a protein, T. vaginalis macrophage migration inhibitory factor (TvMIF), that is 47% similar to human macrophage migration inhibitory factor (HuMIF), a proinflammatory cytokine. Because HuMIF is reported to be elevated in prostate cancer and inflammation plays an important role in the initiation and progression of cancers, we have explored a role for TvMIF in prostate cancer. Here, we show that TvMIF has tautomerase activity, inhibits macrophage migration, and is proinflammatory. We also demonstrate that TvMIF binds the human CD74 MIF receptor with high affinity, comparable to that of HuMIF, which triggers activation of ERK, Akt, and Bcl-2-associated death promoter phosphorylation at a physiologically relevant concentration (1 ng/mL, 80 pM). TvMIF increases the in vitro growth and invasion through Matrigel of benign and prostate cancer cells. Sera from patients infected with T. vaginalis are reactive to TvMIF, especially in males. The presence of anti-TvMIF antibodies indicates that TvMIF is released by the parasite and elicits host immune responses during infection. Together, these data indicate that chronic T. vaginalis infections may result in TvMIF-driven inflammation and cell proliferation, thus triggering pathways that contribute to the promotion and progression of prostate cancer.

Projected Influences of Changes in Weather Severity on Autumn-Winter Distributions of Dabbling Ducks in the Mississippi and Atlantic Flyways during the Twenty-First Century

DOE Office of Scientific and Technical Information (OSTI.GOV)

Notaro, Michael; Schummer, Michael; Zhong, Yafang

Projected changes in the relative abundance and timing of autumn-winter migration are assessed for seven dabbling duck species across the Mississippi and Atlantic Flyways for the mid- and late 21 st century. Species-specific observed relationships are established between cumulative weather severity in autumn-winter and duck population rate of change. Dynamically downscaled projections of weather severity are developed using a high-resolution regional climate model, interactively coupled to a one-dimensional lake model to represent the Great Lakes and associated lake-effect snowfall. Based on the observed relationships and downscaled climate projections of rising air temperatures and reduced snow cover, delayed autumn-winter migration ismore » expected for all species, with the least delays for the Northern Pintail and the greatest delays for the Mallard. Indeed, the Mallard, the most common and widespread duck in North America, may overwinter in the Great Lakes region by the late 21 st century. This highlights the importance of protecting and restoring wetlands across the mid-latitudes of North America, including the Great Lakes Basin, because dabbling ducks are likely to spend more time there, which would impact existing wetlands through increased foraging pressure. Furthermore, inconsistency in the timing and intensity of the traditional autumn-winter migration of dabbling ducks in the Mississippi and Atlantic Flyways could have social and economic consequences to communities to the south, where hunting and birdwatching would be affected.« less

Projected Influences of Changes in Weather Severity on Autumn-Winter Distributions of Dabbling Ducks in the Mississippi and Atlantic Flyways during the Twenty-First Century

DOE PAGES

Notaro, Michael; Schummer, Michael; Zhong, Yafang; ...

2016-12-13

Projected changes in the relative abundance and timing of autumn-winter migration are assessed for seven dabbling duck species across the Mississippi and Atlantic Flyways for the mid- and late 21 st century. Species-specific observed relationships are established between cumulative weather severity in autumn-winter and duck population rate of change. Dynamically downscaled projections of weather severity are developed using a high-resolution regional climate model, interactively coupled to a one-dimensional lake model to represent the Great Lakes and associated lake-effect snowfall. Based on the observed relationships and downscaled climate projections of rising air temperatures and reduced snow cover, delayed autumn-winter migration ismore » expected for all species, with the least delays for the Northern Pintail and the greatest delays for the Mallard. Indeed, the Mallard, the most common and widespread duck in North America, may overwinter in the Great Lakes region by the late 21 st century. This highlights the importance of protecting and restoring wetlands across the mid-latitudes of North America, including the Great Lakes Basin, because dabbling ducks are likely to spend more time there, which would impact existing wetlands through increased foraging pressure. Furthermore, inconsistency in the timing and intensity of the traditional autumn-winter migration of dabbling ducks in the Mississippi and Atlantic Flyways could have social and economic consequences to communities to the south, where hunting and birdwatching would be affected.« less

Projected Influences of Changes in Weather Severity on Autumn-Winter Distributions of Dabbling Ducks in the Mississippi and Atlantic Flyways during the Twenty-First Century.

PubMed

Notaro, Michael; Schummer, Michael; Zhong, Yafang; Vavrus, Stephen; Van Den Elsen, Lena; Coluccy, John; Hoving, Christopher

2016-01-01

Projected changes in the relative abundance and timing of autumn-winter migration are assessed for seven dabbling duck species across the Mississippi and Atlantic Flyways for the mid- and late 21st century. Species-specific observed relationships are established between cumulative weather severity in autumn-winter and duck population rate of change. Dynamically downscaled projections of weather severity are developed using a high-resolution regional climate model, interactively coupled to a one-dimensional lake model to represent the Great Lakes and associated lake-effect snowfall. Based on the observed relationships and downscaled climate projections of rising air temperatures and reduced snow cover, delayed autumn-winter migration is expected for all species, with the least delays for the Northern Pintail and the greatest delays for the Mallard. Indeed, the Mallard, the most common and widespread duck in North America, may overwinter in the Great Lakes region by the late 21st century. This highlights the importance of protecting and restoring wetlands across the mid-latitudes of North America, including the Great Lakes Basin, because dabbling ducks are likely to spend more time there, which would impact existing wetlands through increased foraging pressure. Furthermore, inconsistency in the timing and intensity of the traditional autumn-winter migration of dabbling ducks in the Mississippi and Atlantic Flyways could have social and economic consequences to communities to the south, where hunting and birdwatching would be affected.

Four Decades of Andean Timberline Migration and Implications for Biodiversity Loss with Climate Change

PubMed Central

Lutz, David A.; Powell, Rebecca L.; Silman, Miles R.

2013-01-01

Rapid 21st-century climate change may lead to large population decreases and extinction in tropical montane cloud forest species in the Andes. While prior research has focused on species migrations per se, ecotones may respond to different environmental factors than species. Even if species can migrate in response to climate change, if ecotones do not they can function as hard barriers to species migrations, making ecotone migrations central to understanding species persistence under scenarios of climate change. We examined a 42-year span of aerial photographs and high resolution satellite imagery to calculate migration rates of timberline–the grassland-forest ecotone–inside and outside of protected areas in the high Peruvian Andes. We found that timberline in protected areas was more likely to migrate upward in elevation than in areas with frequent cattle grazing and fire. However, rates in both protected (0.24 m yr−1) and unprotected (0.05 m yr−1) areas are only 0.5–2.3% of the rates needed to stay in equilibrium with projected climate by 2100. These ecotone migration rates are 12.5 to 110 times slower than the observed species migration rates within the same forest, suggesting a barrier to migration for mid- and high-elevation species. We anticipate that the ecotone will be a hard barrier to migration under future climate change, leading to drastic population and biodiversity losses in the region unless intensive management steps are taken. PMID:24040260

Four decades of Andean timberline migration and implications for biodiversity loss with climate change.

PubMed

Lutz, David A; Powell, Rebecca L; Silman, Miles R

2013-01-01

Rapid 21st-century climate change may lead to large population decreases and extinction in tropical montane cloud forest species in the Andes. While prior research has focused on species migrations per se, ecotones may respond to different environmental factors than species. Even if species can migrate in response to climate change, if ecotones do not they can function as hard barriers to species migrations, making ecotone migrations central to understanding species persistence under scenarios of climate change. We examined a 42-year span of aerial photographs and high resolution satellite imagery to calculate migration rates of timberline--the grassland-forest ecotone-inside and outside of protected areas in the high Peruvian Andes. We found that timberline in protected areas was more likely to migrate upward in elevation than in areas with frequent cattle grazing and fire. However, rates in both protected (0.24 m yr(-1)) and unprotected (0.05 m yr(-1)) areas are only 0.5-2.3% of the rates needed to stay in equilibrium with projected climate by 2100. These ecotone migration rates are 12.5 to 110 times slower than the observed species migration rates within the same forest, suggesting a barrier to migration for mid- and high-elevation species. We anticipate that the ecotone will be a hard barrier to migration under future climate change, leading to drastic population and biodiversity losses in the region unless intensive management steps are taken.

75 FR 11864 - Proposed Information Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

... Corporation is soliciting comments concerning its proposed renewal of its Senior Corps Project Progress Report... (e.g., permitting electronic submissions of responses). Background The Progress Report (PPR) was... grant requirements; track and measure progress to benefit the local project and its contributions to...

Magnolol Inhibits Human Glioblastoma Cell Migration by Regulating N-Cadherin.

PubMed

Cheng, Yu-Chen; Tsao, Min-Jen; Chiu, Chen-Yang; Kan, Po-Chieh; Chen, Ying

2018-06-01

Glioblastoma is a primary malignant brain tumor with a poor prognosis. An effective treatment for glioblastoma is needed. Magnolol is a natural compound from Magnolia officinalis suggested to have antiproliferative activity. The aim of this research was to investigate the anticancer effects of magnolol in glioma, with an emphasis on migration and the underlying mechanism. Magnolol decreased the expression of focal adhesion-related proteins and inhibited LN229 and U87MG glioma cell migration. The levels of phosphorylated myosin light chain (p-MLC), phosphorylated myosin light chain kinase and myosin phosphatase target subunit 1 were reduced in response to magnolol treatment. In addition, immunostaining and membrane fractionation showed that the distribution of N-cadherin at the glioma cell membrane was decreased by magnolol. In an orthotropic xenograft animal model, magnolol treatment not only inhibited tumor progression but also reduced p-MLC and N-cadherin protein expression. In conclusion, magnolol reduces cell migration, potentially through regulating focal adhesions and N-cadherin in glioma cells. Magnolol is a potential candidate for glioma treatment.

Epilepsy of infancy with migrating focal seizures: three patients treated with the ketogenic diet.

PubMed

Caraballo, Roberto; Noli, Daniel; Cachia, Pedro

2015-06-01

We present three patients with epilepsy of infancy with migrating focal seizures treated with the ketogenic diet. Between February 1, 2012 and January 31, 2014, three patients who met the diagnostic criteria for migrating focal seizures in infancy at our department were placed on the ketogenic diet and followed for a minimum of seven months. Two of the three children responded well to the ketogenic diet. One of these patients became seizure-free and his neuropsychological performance also significantly improved. The other child had a seizure reduction of 75% to 99% with only weekly seizures and moderate psychomotor improvement. For these two patients who responded well to the ketogenic diet, hospital admission was not required. The remaining patient had a seizure reduction of less than 50%. Tolerability of the diet was good in all three patients. Early treatment with the ketogenic diet should be considered for epilepsy of infancy with migrating focal seizures to control seizures and status epilepticus, and avoid progressive cognitive impairment.

MIIP, a cytoskeleton regulator that blocks cell migration and invasion, delays mitosis, and suppresses tumorogenesis.

PubMed

Wang, Yingmei; Wen, Jing; Zhang, Wei

2011-02-01

The migration and invasion inhibitory protein (MIIP) was initially discovered in a yeast two-hybrid screen for proteins that interact and inhibit the migration and invasion-promoting protein insulin-like growth factor binding protein 2 (IGFBP2). Recent studies have shown that MIIP not only modulates IGFBP2 but also regulates microtubule by binding to and inhibiting HDAC6, a class 2 histone deacetylase that deacetylates α-tubulin, heat-shock protein 90 (HSP90), and cortactin. In addition, MIIP also regulates the mitosis checkpoint, another microtubule-associated process. The location of the MIIP gene in chromosomal region 1p36, a commonly deleted region in a broad spectrum of human cancers, and the observation that MIIP attenuates tumorigenesis in a mouse model suggest that it functions as a tumor-suppressor gene. This review summarizes the recent progress in characterizing this novel protein, which regulates cell migration and mitosis, two processes that rely on the highly coordinated dynamics of the microtubule and cytoskeleton systems.

Suppression of fructose-bisphosphate aldolase C expression as a predictor of advanced oral squamous cell carcinoma.

PubMed

Li, Yue-Ju; Huang, Tse-Hung; Hsiao, Michael; Lin, Been-Ren; Cheng, Shih-Jung; Yang, Cheng-Ning; Lai, Wei-Ting; Wu, Tai-Sheng; Fan, Jia-Ruei; Kuo, Mark Yen-Ping; Chang, Cheng-Chi

2016-04-01

Glycolysis machinery regulates cancer cell behavior. However, the roles of these glycolysis enzymes in oral squamous cell carcinoma (OSCC) progression remain unknown. Fructose-bisphosphate aldolase C (ALDOC) expression in OSCC patients and cell lines was detected using quantitative real-time polymerase chain reaction (PCR). The functions of ALDOC in migration and invasion were determined using gain and loss of function approaches. An orthotopic OSCC animal model was performed to investigate the effects of ALDOC on metastasis and tumorigenesis in vivo. ALDOC expression is negatively significantly correlated with clinical outcome and cell migration in vitro and in vivo. ALDOC blocks adenosine triphosphate generation and lactate production, and mutation constructs of Arg42 and Lys146 functionally restore ALDOC-inhibited cell migration and invasion. ALDOC functions as an OSCC prognosis marker clinically, and suppresses migration and invasion by its catalytic domain of Arg42 and Lys146. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1075-E1085, 2016. © 2015 Wiley Periodicals, Inc.

ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion.

PubMed

Chen, Wei-Ta; Ebelt, Nancy D; Stracker, Travis H; Xhemalce, Blerta; Van Den Berg, Carla L; Miller, Kyle M

2015-06-01

Ataxia-telangiectasia mutated (ATM) protein kinase regulates the DNA damage response (DDR) and is associated with cancer suppression. Here we report a cancer-promoting role for ATM. ATM depletion in metastatic cancer cells reduced cell migration and invasion. Transcription analyses identified a gene network, including the chemokine IL-8, regulated by ATM. IL-8 expression required ATM and was regulated by oxidative stress. IL-8 was validated as an ATM target by its ability to rescue cell migration and invasion defects in ATM-depleted cells. Finally, ATM-depletion in human breast cancer cells reduced lung tumors in a mouse xenograft model and clinical data validated IL-8 in lung metastasis. These findings provide insights into how ATM activation by oxidative stress regulates IL-8 to sustain cell migration and invasion in cancer cells to promote metastatic potential. Thus, in addition to well-established roles in tumor suppression, these findings identify a role for ATM in tumor progression.

Erbb2 up-regulation of ADAM12 expression accelerates skin cancer progression.

PubMed

Rao, Velidi H; Vogel, Kristen; Yanagida, Jodi K; Marwaha, Nitin; Kandel, Amrit; Trempus, Carol; Repertinger, Susan K; Hansen, Laura A

2015-10-01

Solar ultraviolet (UV) radiation can cause severe damage to the skin and is the primary cause of most skin cancer. UV radiation causes DNA damage leading to mutations and also activates the Erbb2/HER2 receptor through indirect mechanisms involving reactive oxygen species. We hypothesized that Erbb2 activation accelerates the malignant progression of UV-induced skin cancer. Following the induction of benign squamous papillomas by UV exposure of v-ras(Ha) transgenic Tg.AC mice, mice were treated topically with the Erbb2 inhibitor AG825 and tumor progression monitored. AG825 treatment reduced tumor volume, increased tumor regression, and delayed the development of malignant squamous cell carcinoma (SCC). Progression to malignancy was associated with increased Erbb2 and ADAM12 (A Disintegin And Metalloproteinase 12) transcripts and protein, while inhibition of Erbb2 blocked the increase in ADAM12 message upon malignant progression. Similarly, human SCC and SCC cell lines had increased ADAM12 protein and transcripts when compared to normal controls. To determine whether Erbb2 up-regulation of ADAM12 contributed to malignant progression of skin cancer, Erbb2 expression was modulated in cultured SCC cells using forced over-expression or siRNA targeting, demonstrating up-regulation of ADAM12 by Erbb2. Furthermore, ADAM12 transfection or siRNA targeting revealed that ADAM12 increased both the migration and invasion of cutaneous SCC cells. Collectively, these results suggest Erbb2 up-regulation of ADAM12 as a novel mechanism contributing to the malignant progression of UV-induced skin cancer. Inhibition of Erbb2/HER2 reduced tumor burden, increased tumor regression, and delayed the progression of benign skin tumors to malignant SCC in UV-exposed mice. Inhibition of Erbb2 suppressed the increase in metalloproteinase ADAM12 expression in skin tumors, which in turn increased migration and tumor cell invasiveness. © 2014 Wiley Periodicals, Inc.

MiR-525-3p Enhances the Migration and Invasion of Liver Cancer Cells by Downregulating ZNF395

PubMed Central

Pang, Fei; Zha, Ruopeng; Zhao, Yingjun; Wang, Qifeng; Chen, Di; Zhang, Zhenfeng; Chen, Taoyang; Yao, Ming; Gu, Jianren; He, Xianghuo

2014-01-01

Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395) is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets. PMID:24599008

Latitudinal migration of sunspots based on the ESAI database

NASA Astrophysics Data System (ADS)

Zhang, Juan; Li, Fu-Yu; Feng, Wen

2018-01-01

The latitudinal migration of sunspots toward the equator, which implies there is propagation of the toroidal magnetic flux wave at the base of the solar convection zone, is one of the crucial observational bases for the solar dynamo to generate a magnetic field by shearing of the pre-existing poloidal magnetic field through differential rotation. The Extended time series of Solar Activity Indices (ESAI) elongated the Greenwich observation record of sunspots by several decades in the past. In this study, ESAI’s yearly mean latitude of sunspots in the northern and southern hemispheres during the years 1854 to 1985 is utilized to statistically test whether hemispherical latitudinal migration of sunspots in a solar cycle is linear or nonlinear. It is found that a quadratic function is statistically significantly better at describing hemispherical latitudinal migration of sunspots in a solar cycle than a linear function. In addition, the latitude migration velocity of sunspots in a solar cycle decreases as the cycle progresses, providing a particular constraint for solar dynamo models. Indeed, the butterfly wing pattern with a faster latitudinal migration rate should present stronger solar activity with a shorter cycle period, and it is located at higher latitudinal position, giving evidence to support the Babcock-Leighton dynamo mechanism.

[Health and Prevention Behaviour of Persons with and without Migration Background].

PubMed

Walter, U; Bisson, S; Gerken, U; Machleidt, W; Krauth, C

2015-09-01

Based on a sample of the Hannover registration office this project analyses the health and prevention behaviour of Russian- and Turkish-language migrants compared to natives. The project analysis revealed considerable differences, particularly concerning addiction behaviour and physical activities. © Georg Thieme Verlag KG Stuttgart · New York.

Assessing the Energy and Emissions Implications of Alternative Population Scenarios Using a State-Level Integrated Assessment Model

EPA Science Inventory

We use GCAM-USA to examine the sensitivity of energy demands and resulting pollutant emissions and health impacts to differing population projections. The population projections are based on future fertility, mortality, migration and education assumptions consistent with the five...

Numerical Modeling Tools for the Prediction of Solution Migration Applicable to Mining Site

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martell, M.; Vaughn, P.

1999-01-06

Mining has always had an important influence on cultures and traditions of communities around the globe and throughout history. Today, because mining legislation places heavy emphasis on environmental protection, there is great interest in having a comprehensive understanding of ancient mining and mining sites. Multi-disciplinary approaches (i.e., Pb isotopes as tracers) are being used to explore the distribution of metals in natural environments. Another successful approach is to model solution migration numerically. A proven method to simulate solution migration in natural rock salt has been applied to project through time for 10,000 years the system performance and solution concentrations surroundingmore » a proposed nuclear waste repository. This capability is readily adaptable to simulate solution migration around mining.« less

An overview of some concepts, potentials, issues, and realities of assisted migration for climate change adaptation in forests

Treesearch

Louis R. Iverson; Matthew P. Peters; Stephen Matthews; Anantha Prasad

2013-01-01

The climate has always been changing, but the rapid rate of climate change, as projected by the IPCC (2007) will likely place unique stresses on plant communities. In addition, anthropogenic barriers (e.g., fragmented land use) present a significant modern constraint that will limit the ability of species migration in responses to a changing climate. As such, managers...

Agricultural development and emigration: rhetoric and reality.

PubMed

Thompson, G; Amon, R; Martin, P L

1986-01-01

"The untested premise of trade liberalizing U.S. development programs such as the Caribbean Basin Initiative is that commodity trade can substitute for international labor migration. Analysis of U.S. tomato producing regions in Sinaloa, Mexico and Florida suggests that the effect of trade liberalization on international labor migration is uncertain." The emphasis is on how such development projects might affect the flow of illegal migrants to the United States. excerpt

Tracking from the tropics reveals behaviour of juvenile songbirds on their first spring migration.

PubMed

McKinnon, Emily A; Fraser, Kevin C; Stanley, Calandra Q; Stutchbury, Bridget J M

2014-01-01

Juvenile songbirds on spring migration travel from tropical wintering sites to temperate breeding destinations thousands of kilometres away with no prior experience to guide them. We provide a first glimpse at the migration timing, routes, and stopover behaviour of juvenile wood thrushes (Hylocichla mustelina) on their inaugural spring migration by using miniaturized archival geolocators to track them from Central America to the U.S. and Canada. We found significant differences between the timing of juvenile migration and that of more experienced adults: juveniles not only departed later from tropical wintering sites relative to adults, they also became progressively later as they moved northward. The increasing delay was driven by more frequent short stops by juveniles along their migration route, particularly in the U.S. as they got closer to breeding sites. Surprisingly, juveniles were just as likely as adults to cross the Gulf of Mexico, an open-water crossing of 800-1000 km, and migration route at the Gulf was not significantly different for juveniles relative to adults. To determine if the later departure of juveniles was related to poor body condition in winter relative to adults, we examined percent lean body mass, fat scores, and pectoral muscle scores of juvenile versus adult birds at a wintering site in Belize. We found no age-related differences in body condition. Later migration timing of juveniles relative to adults could be an adaptive strategy (as opposed to condition-dependent) to avoid the high costs of fast migration and competition for breeding territories with experienced and larger adults. We did find significant differences in wing size between adults and juveniles, which could contribute to lower flight efficiency of juveniles and thus slower overall migration speed. We provide the first step toward understanding the "black box" of juvenile songbird migration by documenting their migration timing and en route performance.

Tracking from the Tropics Reveals Behaviour of Juvenile Songbirds on Their First Spring Migration

PubMed Central

McKinnon, Emily A.; Fraser, Kevin C.; Stanley, Calandra Q.; Stutchbury, Bridget J. M.

2014-01-01

Juvenile songbirds on spring migration travel from tropical wintering sites to temperate breeding destinations thousands of kilometres away with no prior experience to guide them. We provide a first glimpse at the migration timing, routes, and stopover behaviour of juvenile wood thrushes (Hylocichla mustelina) on their inaugural spring migration by using miniaturized archival geolocators to track them from Central America to the U.S. and Canada. We found significant differences between the timing of juvenile migration and that of more experienced adults: juveniles not only departed later from tropical wintering sites relative to adults, they also became progressively later as they moved northward. The increasing delay was driven by more frequent short stops by juveniles along their migration route, particularly in the U.S. as they got closer to breeding sites. Surprisingly, juveniles were just as likely as adults to cross the Gulf of Mexico, an open-water crossing of 800–1000 km, and migration route at the Gulf was not significantly different for juveniles relative to adults. To determine if the later departure of juveniles was related to poor body condition in winter relative to adults, we examined percent lean body mass, fat scores, and pectoral muscle scores of juvenile versus adult birds at a wintering site in Belize. We found no age-related differences in body condition. Later migration timing of juveniles relative to adults could be an adaptive strategy (as opposed to condition-dependent) to avoid the high costs of fast migration and competition for breeding territories with experienced and larger adults. We did find significant differences in wing size between adults and juveniles, which could contribute to lower flight efficiency of juveniles and thus slower overall migration speed. We provide the first step toward understanding the “black box” of juvenile songbird migration by documenting their migration timing and en route performance. PMID:25141193

Epitaxially grown collagen fibrils reveal diversity in contact guidance behavior among cancer cells.

PubMed

Wang, Juan; Petefish, Joseph W; Hillier, Andrew C; Schneider, Ian C

2015-01-01

Invasion of cancer cells into the surrounding tissue is an important step during cancer progression and is driven by cell migration. Cell migration can be random, but often it is directed by various cues such as aligned fibers composed of extracellular matrix (ECM), a process called contact guidance. During contact guidance, aligned fibers bias migration along the long axis of the fibers. These aligned fibers of ECM are commonly composed of type I collagen, an abundant structural protein around tumors. In this paper, we epitaxially grew several different patterns of organized type I collagen on mica and compared the morphology and contact guidance behavior of two invasive breast cancer cell lines (MDA-MB-231 and MTLn3 cells). Others have shown that these cells randomly migrate in qualitatively different ways. MDA-MB-231 cells exert large traction forces, tightly adhere to the ECM, and migrate with spindle-shaped morphology and thus adopt a mesenchymal mode of migration. MTLn3 cells exert small traction forces, loosely adhere to the ECM, and migrate with a more rounded morphology and thus adopt an amoeboid mode of migration. As the degree of alignment of type I collagen fibrils increases, cells become more elongated and engage in more directed contact guidance. MDA-MB-231 cells perceive the directional signal of highly aligned type I collagen fibrils with high fidelity, elongating to large extents and migrating directionally. Interestingly, behavior in MTLn3 cells differs. While highly aligned type I collagen fibril patterns facilitate spreading and random migration of MTLn3 cells, they do not support elongation or directed migration. Thus, different contact guidance cues bias cell migration differently and the fidelity of contact guidance is cell type dependent, suggesting that ECM alignment is a permissive cue for contact guidance, but requires a cell to have certain properties to interpret that cue.

SANTA MONICA BAY RESTORATION PROJECT IMPLEMENTATION REVIEW 2002

EPA Science Inventory

The Santa Monica Bay Restoration Project Implementation Review (IR) summarizes the progress and challenges ahead for the Project through examination of it activities in relation to the CCMP. Contents of the IR include: implementation progress with a financing for plan implementat...

BridgeUP: STEM. Creating Opportunities for Women through Tiered Mentorship

NASA Astrophysics Data System (ADS)

Secunda, Amy; Cornelis, Juliette; Ferreira, Denelis; Gomez, Anay; Khan, Ariba; Li, Anna; Soo, Audrey; Mac Low, Mordecai

2018-01-01

BridgeUP: STEM is an ambitious, and exciting initiative responding to the extensive gender and opportunity gaps that exist in the STEM pipeline for women, girls, and under-resourced youth. BridgeUP: STEM has developed a distinct identity in the landscape of computer science education by embedding programming in the context of scientific research. One of the ways in which this is accomplished is through a tiered mentorship program. Five Helen Fellows are chosen from a pool of female, postbaccalaureate applicants to be mentored by researchers at the American Museum of Natural History in a computational research project. The Helen Fellows then act as mentors to six high school women (Brown Scholars), guiding them through a computational project aligned with their own research. This year, three of the Helen Fellows, and by extension, eighteen Brown Scholars, are performing computational astrophysics research. This poster presents one example of a tiered mentorship working on modeling the migration of stellar mass black holes (BH) in active galactic nucleus (AGN) disks. Making an analogy from the well-studied migration and formation of planets in protoplanetary disks to the newer field of migration and formation of binary BH in AGN disks, the Helen Fellow is working with her mentors to make the necessary adaptations of an N-body code incorporating migration torques from the protoplanetary disk case to the AGN disk case to model how binary BH form. This is in order to better understand and make predictions for gravitational wave observations from the Laser Interferometer Gravitational-Wave Observatory (LIGO). The Brown Scholars then implement the Helen Fellow’s code for a variety of different distributions of initial stellar mass BH populations that they generate using python, and produce visualizations of the output to be used in a published paper. Over the course of the project, students will develop a basic understanding of the physics related to their project and develop their practical computational skills.

Utilization Probability Map for Migrating Bald Eagles in Northeastern North America: A Tool for Siting Wind Energy Facilities and Other Flight Hazards

PubMed Central

Mojica, Elizabeth K.; Watts, Bryan D.; Turrin, Courtney L.

2016-01-01

Collisions with anthropogenic structures are a significant and well documented source of mortality for avian species worldwide. The bald eagle (Haliaeetus leucocephalus) is known to be vulnerable to collision with wind turbines and federal wind energy guidelines include an eagle risk assessment for new projects. To address the need for risk assessment, in this study, we 1) identified areas of northeastern North America utilized by migrating bald eagles, and 2) compared these with high wind-potential areas to identify potential risk of bald eagle collision with wind turbines. We captured and marked 17 resident and migrant bald eagles in the northern Chesapeake Bay between August 2007 and May 2009. We produced utilization distribution (UD) surfaces for 132 individual migration tracks using a dynamic Brownian bridge movement model and combined these to create a population wide UD surface with a 1 km cell size. We found eagle migration movements were concentrated within two main corridors along the Appalachian Mountains and the Atlantic Coast. Of the 3,123 wind turbines ≥100 m in height in the study area, 38% were located in UD 20, and 31% in UD 40. In the United States portion of the study area, commercially viable wind power classes overlapped with only 2% of the UD category 20 (i.e., the areas of highest use by migrating eagles) and 4% of UD category 40. This is encouraging because it suggests that wind energy development can still occur in the study area at sites that are most viable from a wind power perspective and are unlikely to cause significant mortality of migrating eagles. In siting new turbines, wind energy developers should avoid the high-use migration corridors (UD categories 20 & 40) and focus new wind energy projects on lower-risk areas (UD categories 60–100). PMID:27336482

Borders as membranes :metaphors and models for improved policy in border regions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Malczynski, Leonard A.; Passell, Howard David; Forster, Craig B.

Political borders are controversial and contested spaces. In an attempt to better understand movement along and through political borders, this project applied the metaphor of a membrane to look at how people, ideas, and things ''move'' through a border. More specifically, the research team employed this metaphor in a system dynamics framework to construct a computer model to assess legal and illegal migration on the US-Mexico border. Employing a metaphor can be helpful, as it was in this project, to gain different perspectives on a complex system. In addition to the metaphor, the multidisciplinary team utilized an array of methodsmore » to gather data including traditional literature searches, an experts workshop, a focus group, interviews, and culling expertise from the individuals on the research team. Results from the qualitative efforts revealed strong social as well as economic drivers that motivate individuals to cross the border legally. Based on the information gathered, the team concluded that legal migration dynamics were of a scope we did not want to consider hence, available demographic models sufficiently capture migration at the local level. Results from both the quantitative and qualitative data searches were used to modify a 1977 border model to demonstrate the dynamic nature of illegal migration. Model runs reveal that current US-policies based on neo-classic economic theory have proven ineffective in curbing illegal migration, and that proposed enforcement policies are also likely to be ineffective. We suggest, based on model results, that improvement in economic conditions within Mexico may have the biggest impact on illegal migration to the U.S. The modeling also supports the views expressed in the current literature suggesting that demographic and economic changes within Mexico are likely to slow illegal migration by 2060 with no special interventions made by either government.« less

Correlation of foodstuffs with ethanol-water mixtures with regard to the solubility of migrants from food contact materials.

PubMed

Seiler, Annika; Bach, Aurélie; Driffield, Malcolm; Paseiro Losada, Perfecto; Mercea, Peter; Tosa, Valer; Franz, Roland

2014-01-01

Today most foods are available in a packed form. During storage, the migration of chemical substances from food packaging materials into food may occur and may therefore be a potential source of consumer exposure. To protect the consumer, standard migration tests are laid down in Regulation (EU) No. 10/2011. When using those migration tests and applying additional conservative conventions, estimated exposure is linked with large uncertainties including a certain margin of safety. Thus the research project FACET was initiated within the 7th Framework Programme of the European Commission with the aim of developing a probabilistic migration modelling framework which allows one (1) to calculate migration into foods under real conditions of use; and (2) to deliver realistic concentration estimates for consumer exposure modelling for complex packaging materials (including multi-material multilayer structures). The aim was to carry out within the framework of the FACET project a comprehensive systematic study on the solubility behaviour of foodstuffs for potentially migrating organic chemicals. Therefore a rapid and convenient method was established to obtain partition coefficients between polymer and food, KP/F. With this method approximately 700 time-dependent kinetic experiments from spiked polyethylene films were performed using model migrants, foods and ethanol-water mixtures. The partition coefficients of migrants between polymer and food (KP/F) were compared with those obtained using ethanol-water mixtures (KP/F's) to investigate whether an allocation of food groups with common migration behaviour to certain ethanol-water mixtures could be made. These studies have confirmed that the solubility of a migrant is mainly dependent on the fat content in the food and on the ethanol concentration of ethanol-water mixtures. Therefore dissolution properties of generic food groups for migrants can be assigned to those of ethanol-water mixtures. All foodstuffs (including dry foods) when allocated to FACET model food group codes can be classified into a reduced number of food categories each represented by a corresponding ethanol-water equivalency.

Utilization Probability Map for Migrating Bald Eagles in Northeastern North America: A Tool for Siting Wind Energy Facilities and Other Flight Hazards.

PubMed

Mojica, Elizabeth K; Watts, Bryan D; Turrin, Courtney L

2016-01-01

Collisions with anthropogenic structures are a significant and well documented source of mortality for avian species worldwide. The bald eagle (Haliaeetus leucocephalus) is known to be vulnerable to collision with wind turbines and federal wind energy guidelines include an eagle risk assessment for new projects. To address the need for risk assessment, in this study, we 1) identified areas of northeastern North America utilized by migrating bald eagles, and 2) compared these with high wind-potential areas to identify potential risk of bald eagle collision with wind turbines. We captured and marked 17 resident and migrant bald eagles in the northern Chesapeake Bay between August 2007 and May 2009. We produced utilization distribution (UD) surfaces for 132 individual migration tracks using a dynamic Brownian bridge movement model and combined these to create a population wide UD surface with a 1 km cell size. We found eagle migration movements were concentrated within two main corridors along the Appalachian Mountains and the Atlantic Coast. Of the 3,123 wind turbines ≥100 m in height in the study area, 38% were located in UD 20, and 31% in UD 40. In the United States portion of the study area, commercially viable wind power classes overlapped with only 2% of the UD category 20 (i.e., the areas of highest use by migrating eagles) and 4% of UD category 40. This is encouraging because it suggests that wind energy development can still occur in the study area at sites that are most viable from a wind power perspective and are unlikely to cause significant mortality of migrating eagles. In siting new turbines, wind energy developers should avoid the high-use migration corridors (UD categories 20 & 40) and focus new wind energy projects on lower-risk areas (UD categories 60-100).

7 CFR 3402.23 - Documentation of progress on funded projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

...: Graduates; is officially terminated from the Fellowship or the academic program due to unsatisfactory academic progress; or voluntarily withdraws from the Fellowship or the academic program. If a Fellow has... Research Information System (CRIS). The CRIS database contains narrative project information, progress...

Gas-controlled seafloor doming on Opouawe Bank, offshore New Zealand

NASA Astrophysics Data System (ADS)

Koch, Stephanie; Berndt, Christian; Bialas, Joerg; Haeckel, Matthias; Crutchley, Gareth; Papenberg, Cord; Klaeschen, Dirk; Greinert, Jens

2015-04-01

The process of gas accumulation and subsequent sediment doming appears to be a precursory process in the development of methane seep sites on Opouawe Bank and might be a common characteristic for gas seeps in general. Seabed domes appear as unimpressive topographic highs with diameters ranging from 10-1000 m and exhibit small vertical displacements and layer thickness in comparison to their width. The dome-like uplift of the sediments results from an increase in pore pressure caused by gas accumulation in near-seabed sediments. In this context sediment doming is widely discussed to be a precursor of pockmark formation. Our results suggest that by breaching of domed seafloor sediments a new seep site can develop and contrary to ongoing discussion does not necessarily lead to the formation of pockmarks. There are clear differences in individual gas migration structures that indicate a progression through different evolutionary stages, which range from channeled gas flow and associated seismic blanking, to gas trapping beneath relatively low-permeability horizons, and finally overpressure accumulation and doming. We present high resolution sub-bottom profiler (Parasound) and 2D multichannel seismic data from Opouawe Bank, an accretionary ridge at the Hikurangi Margin, offshore New Zealand's North Island. Beneath this bank, methane migrates along stratigraphic pathways from a maximum source depth of 1500-2100 mbsf (meter below seafloor) towards active cold seeps at the seafloor. We show that, in the shallow sediment of the upper 100 mbsf, this primary migration mechanism changes into a process of gas accumulation leading to sediment doming. Modeling the height of the gas column necessary to create different dome geometries, shows that doming due to gas accumulation is feasible and consistent with field observations. The well-stratified, sub-horizontal strata that exist beneath Opouawe Bank provide favorable conditions for this type of seep development because shallow sub-vertical gas migration is forced to traverse sedimentary layering in the absence of faults that might otherwise have provided more efficient gas migration pathways. Thus, gas has to generate its own migration pathways through the progressive process of doming and breaking through the strata. The data from offshore New Zealand document that shallow sediment doming does not have to be associated with seafloor pockmarks and that models in which fluid migration through soft sediments necessarily culminates in pockmark formations are not applicable everywhere.

Health and demographic surveillance systems: contributing to an understanding of the dynamics in migration and health

PubMed Central

Gerritsen, Annette; Bocquier, Philippe; White, Michael; Mbacké, Cheikh; Alam, Nurul; Beguy, Donatien; Odhiambo, Frank; Sacoor, Charfudin; Phuc, Ho Dang; Punpuing, Sureeporn; Collinson, Mark A.

2013-01-01

Background Migration is difficult to measure because it is highly repeatable. Health and Demographic Surveillance Systems (HDSSs) provide a unique opportunity to study migration as multiple episodes of migration are captured over time. A conceptual framework is needed to show the public health implications of migration. Objective/design Research conducted in seven HDSS centres [International Network for the Demographic Evaluation of Populations and Their Health (INDEPTH) Network], published in a peer-reviewed volume in 2009, is summarised focussing on the age–sex profile of migrants, the relation between migration and livelihoods, and the impact of migration on health. This illustrates the conceptual structure of the implications of migration. The next phase is described, the Multi-centre Analysis of the Dynamics In Migration And Health (MADIMAH) project, consisting of workshops focussed on preparing data and conducting the analyses for comparative studies amongst HDSS centres in Africa and Asia. The focus here is on the (standardisation of) determinants of migration and the impact of migration on adult mortality. Results The findings in the volume showed a relatively regular age structure for migration among all HDSS centres. Furthermore, migration generally contributes to improved living conditions at the place of origin. However, there are potential negative consequences of migration on health. It was concluded that there is a need to compare results from multiple centres using uniform covariate definitions as well as longitudinal analysis techniques. This was the starting point for the on-going MADIMAH initiative, which has increased capacity at the participating HDSS centres to produce the required datasets and conduct the analyses. Conclusions HDSS centres brought together within INDEPTH Network have already provided strong evidence of the potential negative consequences of migration on health, which contrast with the beneficial impacts of migration on livelihoods. Future comparative evidence using standardised tools will help design policies for mitigating the negative effects, and enhancing the positive effects, of migration on health. PMID:23849188

Practical aspects of prestack depth migration with finite differences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ober, C.C.; Oldfield, R.A.; Womble, D.E.

1997-07-01

Finite-difference, prestack, depth migrations offers significant improvements over Kirchhoff methods in imaging near or under salt structures. The authors have implemented a finite-difference prestack depth migration algorithm for use on massively parallel computers which is discussed. The image quality of the finite-difference scheme has been investigated and suggested improvements are discussed. In this presentation, the authors discuss an implicit finite difference migration code, called Salvo, that has been developed through an ACTI (Advanced Computational Technology Initiative) joint project. This code is designed to be efficient on a variety of massively parallel computers. It takes advantage of both frequency and spatialmore » parallelism as well as the use of nodes dedicated to data input/output (I/O). Besides giving an overview of the finite-difference algorithm and some of the parallelism techniques used, migration results using both Kirchhoff and finite-difference migration will be presented and compared. The authors start out with a very simple Cartoon model where one can intuitively see the multiple travel paths and some of the potential problems that will be encountered with Kirchhoff migration. More complex synthetic models as well as results from actual seismic data from the Gulf of Mexico will be shown.« less

The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration.

PubMed

Wang, Xiangming; Liu, Jianhong; Zhu, Zhiwen; Ou, Guangshuo

2015-03-15

Directional cell migration is fundamental for neural development, and extracellular factors are pivotal for this process. Heparan sulfate proteoglycans (HSPGs) that carry long chains of differentially modified sugar residues contribute to extracellular matrix; however, the functions of HSPG in guiding cell migration remain elusive. Here, we used the Caenorhabditis elegans mutant pool from the Million Mutation Project and isolated a mutant allele of the heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5. Loss-of-function of this enzyme resulted in defective Q neuroblast migration. We showed that hse-5 controlled Q cell migration in a cell non-autonomous manner. By performing live cell imaging in hse-5 mutant animals, we found that hse-5 controlled initial polarization during Q neuroblast migration. Furthermore, our genetic epistasis analysis demonstrated that lon-2 might act downstream of hse-5. Finally, rescue of the hse-5 mutant phenotype by expression of human and mouse hse-5 homologs suggested a conserved function for this gene in neural development. Taken together, our results indicated that proper HSPG modification in the extracellular matrix by HSE-5 is essential for neuroblast polarity during migration. Copyright © 2015 Elsevier Inc. All rights reserved.

The fertility of internal migrants to Kinshasa.

PubMed

Anglewicz, Philip; Corker, Jamaica; Kayembe, Patrick

2017-01-01

The rapid population growth of many African cities has important implications for population health, yet little is known about factors contributing to increasing population, such as the fertility of internal migrants. We examine whether in-migrants to Kinshasa have different fertility patterns than lifetime Kinshasa residents, and identify characteristics of migrants that may explain differences in fertility. We also use detailed migration histories to examine whether fertility differs by features of migration. We use representative data from the PMA2020 Project for 2197 women in Kinshasa, including 340 women who moved to Kinshasa. We examine differences between migrants and non-migrants in fertility and other fertility-related characteristics. We also examine whether fertility differs by duration of residence in Kinshasa, number of lifetime moves, age at first migration, urban/rural classification of birthplace, and the distinction between intra-Kinshasa migration and migration to Kinshasa.. Migrants have significantly higher fertility than permanent Kinshasa residents, but the difference is relatively small in magnitude. This higher fertility appears due in part to patterns of contraceptive use among migrants. There is noteworthy heterogeneity among migrants: higher fertility among migrants is associated with longer duration in Kinshasa, more lifetime moves, urban-Kinshasa migration, older age at first migration, and moving to Kinshasa from outside (as opposed to intra-Kinshasa migration).

7 CFR 3402.23 - Documentation of progress on funded projects.

Code of Federal Regulations, 2011 CFR

2011-01-01

... the academic program due to unsatisfactory academic progress; or voluntarily withdraws from the Fellowship or the academic program. If a Fellow has not completed all degree requirements at the end of the... database contains narrative project information, progress/impact statements, and final technical reports...

7 CFR 3402.23 - Documentation of progress on funded projects.

Code of Federal Regulations, 2014 CFR

2014-01-01

... the academic program due to unsatisfactory academic progress; or voluntarily withdraws from the Fellowship or the academic program. If a Fellow has not completed all degree requirements at the end of the... database contains narrative project information, progress/impact statements, and final technical reports...

7 CFR 3402.23 - Documentation of progress on funded projects.

Code of Federal Regulations, 2012 CFR

2012-01-01

... the academic program due to unsatisfactory academic progress; or voluntarily withdraws from the Fellowship or the academic program. If a Fellow has not completed all degree requirements at the end of the... database contains narrative project information, progress/impact statements, and final technical reports...

7 CFR 3402.23 - Documentation of progress on funded projects.

Code of Federal Regulations, 2013 CFR

2013-01-01

... the academic program due to unsatisfactory academic progress; or voluntarily withdraws from the Fellowship or the academic program. If a Fellow has not completed all degree requirements at the end of the... database contains narrative project information, progress/impact statements, and final technical reports...

Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2003-2004 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, Steven G.; Muir, William D.; Zabel, Richard W.

2004-01-01

For juvenile chinook salmon Oncorhynchus tshawytscha, sockeye salmon O. nerka, and steelhead O. mykiss that migrate through reservoirs, hydroelectric projects, and free-flowing sections of the Snake and Columbia Rivers, survival estimates are essential to develop effective strategies for recovering depressed stocks. Many management strategies were based on estimates of system survival (Raymond 1979; Sims and Ossiander 1981) derived in a river system considerably different from today's (Williams and Matthews 1995; Williams et al. 2001). Knowledge of the magnitude, locations, and causes of smolt mortality under present passage conditions, and under conditions projected for the future, are necessary to develop strategiesmore » that will optimize smolt survival during migration. From 1993 through 2002, the National Marine Fisheries Service (NMFS) and the University of Washington (UW) demonstrated the feasibility of using three statistical models to estimate survival of PIT-tagged (Prentice et al. 1990a) juvenile salmonids passing through Snake River dams and reservoirs (Iwamoto et al. 1994; Muir et al. 1995, 1996, 2001a, 2003; Smith et al. 1998, 2000a,b; Hockersmith et al. 1999; Zabel et al. 2001, 2002). Evaluation of assumptions for these models indicated that all were generally satisfied, and accurate and precise survival estimates were obtained. In 2003, NMFS and UW completed the eleventh year of the study. Flow levels during the early portion of the 2003 spring migration were similar to 2002, and only slightly higher than in the drought conditions during 2001. However, flow levels were much greater during the later part of the migration in 2003. Spill levels were similar to 2002, much higher than in 2001. Research objectives were to: (1) estimate reach survival and travel time in the Snake and Columbia Rivers throughout the yearling chinook salmon and steelhead migrations; (2) evaluate relationships between survival estimates and migration conditions; and (3) evaluate the performance of the survival-estimation models under prevailing operational and environmental conditions. Additionally, as adult return information becomes available, as part of this study we will evaluate relationships between juvenile survival and subsequent adult returns for fish with different juvenile migration histories.« less

Polycyclic aromatic hydrocarbon (PAH)-mediated upregulation of hepatic microRNA-181 family promotes cancer cell migration by targeting MAPK phosphatase-5, regulating the activation of p38 MAPK

DOE Office of Scientific and Technical Information (OSTI.GOV)

Song, Mi-Kyung; School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seoungbuk-Gu, Seoul 136-701; Park, Yong-Keun

2013-11-15

Growing evidence indicates that changes in microRNA (miRNA) expression in cancer induced by chemical carcinogens play an important role in cancer development and progression by regulating related genes. However, the mechanisms underlying miRNA involvement in hepatocarcinogenesis induced by polycyclic aromatic hydrocarbons (PAHs) remain unclear. Thus, the identification of aberrant miRNA expression during PAH-induced cancer cell migration will lead to a better understanding of the substantial role of miRNAs in cancer progression. In the present study, miRNA expression profiling showed significant upregulation of miR-181a, -181b, and -181d in human hepatocellular carcinoma cells (HepG2 line) exposed to benzo[a]anthracene (BA) and benzo[k]fluoranthene (BF).more » MAPK phosphatase-5 (MKP-5), a validated miR-181 target that deactivates MAPKs, was markedly suppressed while phosphorylation of p38 MAPK was increased after BA and BF exposure. The migration of HepG2 cells, observed using the scratch wound-healing assay, also increased in a dose-dependent manner. Depletion of miR-181 family members by miRNA inhibitors enhanced the expression of MKP-5 and suppressed the phosphorylation of p38 MAPK. Furthermore, the depletion of the miR-181 family inhibited cancer cell migration. Based on these results, we conclude that the miR-181 family plays a critical role in PAH-induced hepatocarcinogenesis by targeting MKP-5, resulting in the regulation of p38 MAPK activation. - Highlights: • We found significant upregulation of miR-181 family in HCC exposed to BA and BF. • We identified the MKP-5 as a putative target of miR-181 family. • MKP-5 was suppressed while p-P38 was increased after BA and BF exposure. • The migration of HepG2 cells increased in a dose-dependent manner.« less

Association of Wnt1-inducible signaling pathway protein-1 with the proliferation, migration and invasion in gastric cancer cells.

PubMed

Jia, Shuqin; Qu, Tingting; Feng, Mengmeng; Ji, Ke; Li, Ziyu; Jiang, Wenguo; Ji, Jiafu

2017-06-01

Wnt1-inducible signaling pathway protein-1 is a cysteine-rich protein that belongs to the CCN family, which has been implicated in mediating the occurrence and progression through distinct molecular mechanisms in several tumor types. However, the association of Wnt1-inducible signaling pathway protein-1 with gastric cancer and the related molecular mechanisms remain to be elucidated. Therefore, this study aimed to clarify the biological role of Wnt1-inducible signaling pathway protein-1 in the proliferation, migration, and invasion in gastric cancer cells and further investigated the associated molecular mechanism on these biological functions. We first detected the expression level of Wnt1-inducible signaling pathway protein-1 in gastric cancer, and the reverse transcription polymerase chain reaction have shown that Wnt1-inducible signaling pathway protein-1 expression levels were upregulated in gastric cancer tissues. The expression of Wnt1-inducible signaling pathway protein-1 in gastric cancer cell lines was also detected by quantitative real-time polymerase chain reaction and Western blotting. Furthermore, two gastric cancer cell lines with high expression of Wnt1-inducible signaling pathway protein-1 were selected to explore the biological function of Wnt1-inducible signaling pathway protein-1 in gastric cancer. Function assays indicated that knockdown of Wnt1-inducible signaling pathway protein-1 suppressed cell proliferation, migration, and invasion in BGC-823 and AGS gastric cancer cells. Further investigation of mechanisms suggested that cyclinD1 was identified as one of Wnt1-inducible signaling pathway protein-1 related genes to accelerate proliferation in gastric cancer cells. In addition, one pathway of Wnt1-inducible signaling pathway protein-1 induced migration and invasion was mainly through the enhancement of epithelial-to-mesenchymal transition progression. Taken together, our findings presented the first evidence that Wnt1-inducible signaling pathway protein-1 was upregulated in gastric cancer and acted as an oncogene by promoting proliferation, migration, and invasion in gastric cancer cells.

MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Xin; Wei, Min; Wang, Wei, E-mail: rjwangwei@126.com

2013-08-09

Highlights: •miR-340 is downregulated in OS cell lines and tissues. •miR-340 suppresses OS cell proliferation, migration and invasion. •miR-340 suppresses tumor growth and metastasis of OS cells in nude mice. •ROCK1 is a target gene of miR-340. •ROCK1 is involved in miR-340-induced suppression of OS cell proliferation, migration and invasion. -- Abstract: MicroRNAs (miRNAs) play key roles in cancer development and progression. In the present study, we investigated the role of miR-340 in the progression and metastasis of osteosarcoma (OS). Our results showed that miR-340 was frequently downregulated in OS tumors and cell lines. Overexpression of miR-340 in OS cellmore » lines significantly inhibited cell proliferation, migration, and invasion in vitro, and tumor growth and metastasis in a xenograft mouse model. ROCK1 was identified as a target of miR-340, and ectopic expression of miR-340 downregulated ROCK1 by direct binding to its 3′ untranslated region. siRNA-mediated silencing of ROCK1 phenocopied the effects of miR-340 overexpression, whereas restoration of ROCK1 in miR-340-overexpressing OS cells reversed the suppressive effects of miR-340. Together, these findings indicate that miR-340 acts as a tumor suppressor and its downregulation in tumor tissues may contribute to the progression and metastasis of OS through a mechanism involving ROCK1, suggesting miR-340 as a potential new diagnostic and therapeutic target for the treatment of OS.« less

Bladder cancer exosomes contain EDIL-3/Del1 and facilitate cancer progression.

PubMed

Beckham, Carla J; Olsen, Jayme; Yin, Peng-Nien; Wu, Chia-Hao; Ting, Huei-Ju; Hagen, Fred K; Scosyrev, Emelian; Messing, Edward M; Lee, Yi-Fen

2014-08-01

High grade bladder cancer is an extremely aggressive malignancy associated with high rates of morbidity and mortality. Understanding how exosomes may affect bladder cancer progression could reveal novel therapeutic targets. Exosomes derived from human bladder cancer cell lines and the urine of patients with high grade bladder cancer were assessed for the ability to promote cancer progression in standard assays. Exosomes purified from the high grade bladder cancer cell line TCC-SUP and the nonmalignant urothelial cell line SV-HUC were submitted for mass spectrometry analysis. EDIL-3 was identified and selected for further analysis. Western blot was done to determine EDIL-3 levels in urinary exosomes from patients with high grade bladder cancer. shRNA gene knockdown and recombinant EDIL-3 were applied to study EDIL-3 function. Exosomes isolated from high grade bladder cancer cells and the urine of patients with high grade bladder cancer promoted angiogenesis and migration of bladder cancer cells and endothelial cells. We silenced EDIL-3 expression and found that shEDIL-3 exosomes did not facilitate angiogenesis, and urothelial and endothelial cell migration. Moreover, exosomes purified from the urine of patients with high grade bladder cancer contained significantly higher EDIL-3 levels than exosomes from the urine of healthy controls. EDIL-3 activated epidermal growth factor receptor signaling while blockade of epidermal growth factor receptor signaling abrogated this EDIL-3 induced bladder cell migration. Exosomes derived from the urine of patients with bladder cancer contains bioactive molecules such as EDIL-3. Identifying these components and their associated oncogenic pathways could lead to novel therapeutic targets and treatment strategies. Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

'Decoy' and 'non-decoy' functions of DcR3 promote malignant potential in human malignant fibrous histiocytoma cells.

PubMed

Toda, Mitsunori; Kawamoto, Teruya; Ueha, Takeshi; Kishimoto, Kenta; Hara, Hitomi; Fukase, Naomasa; Onishi, Yasuo; Harada, Risa; Minoda, Masaya; Kurosaka, Masahiro; Akisue, Toshihiro

2013-09-01

Decoy receptor 3 (DcR3) is a soluble secreted protein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. DcR3 inhibits the Fas ligand (FasL)/Fas apoptotic pathway by binding to FasL, competitively with Fas receptor. Previous studies have reported that overexpression of DcR3 has been detected in various human malignancies and that DcR3 functions as a 'decoy' for FasL to inhibit FasL-induced apoptosis. In addition, recent studies have revealed that DcR3 has 'non-decoy' functions to promote tumor cell migration and invasion, suggesting that DcR3 may play important roles in tumor progression by decoy and non-decoy functions. We have previously reported that overexpression of DcR3 was observed in human malignant fibrous histiocytoma (MFH), however, the roles of DcR3 in MFH have not been studied. In the present study, to elucidate the roles of DcR3 in tumor progression of MFH, we examined the effects of DcR3 inhibition on cell apoptosis, migration and invasion in human MFH cells. siRNA knockdown of DcR3 enhanced the FasL-induced apoptotic activity and significantly decreased cell migration and invasion with a decrease in the activation of phosphatidylinositol 3 kinase (PI3K)/Akt and matrix metalloproteinase (MMP)-2. The findings in this study strongly suggest that DcR3 plays important roles in tumor progression of human MFH by decoy as well as non-decoy functions and that DcR3 may serve as a potent therapeutic target for human MFH.

Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients

PubMed Central

Zhu, Junyan; Trillsch, Fabian; Mayr, Doris; Kuhn, Christina; Rahmeh, Martina; Hofmann, Simone; Vogel, Marianne; Mahner, Sven; Jeschke, Udo; von Schönfeldt, Viktoria

2018-01-01

Background Prostaglandin E2 (PGE2) receptor 3 (EP3) regulates tumor cell proliferation, migration, and invasion in numerous cancers. The role of EP3 as a prognostic biomarker in endometrial cancer remains unclear. The primary aim of this study was to analyze the prognostic significance of EP3 expression in endometrial cancer. Methods We analyzed the EP3 expression of 140 endometrial carcinoma patients by immunohistochemistry. RL95-2 endometrial cancer cell line was chosen from four endometrial cancer cell lines (RL95-2, Ishikawa, HEC-1-A, and HEC-1-B) according to EP3 expression level. Treated with PGE2 and EP3 antagonist, RL95-2 cells were investigated by MTT, BrdU, and wound healing assay for functional assessment of EP3. Results EP3 staining differed significantly according to WHO tumor grading in both whole cohort (p = 0.01) and the subgroup of endometrioid carcinoma (p = 0.01). Patients with high EP3 expression in their respective tumors had impaired progression-free survival as well as overall survival in both cohorts above. EP3 expression in the overall cohort was identified as an independent prognostic marker for progression-free survival (HR 1.014, 95%CI 1.003-1.024, p = 0.01) when adjusted for age, stage, grading, and recurrence. Treatment with EP3 antagonists induced upregulation of estrogen receptor β and decreased activity of Ras and led to attenuated proliferation and migration of RL95-2 cells. Conclusions EP3 seems to play a crucial role in endometrial cancer progression. In the context of limited systemic treatment options for endometrial cancer, this explorative analysis identifies EP3 as a potential target for diagnostic workup and therapy. PMID:29416671

Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients.

PubMed

Zhu, Junyan; Trillsch, Fabian; Mayr, Doris; Kuhn, Christina; Rahmeh, Martina; Hofmann, Simone; Vogel, Marianne; Mahner, Sven; Jeschke, Udo; von Schönfeldt, Viktoria

2018-01-02

Prostaglandin E2 (PGE2) receptor 3 (EP3) regulates tumor cell proliferation, migration, and invasion in numerous cancers. The role of EP3 as a prognostic biomarker in endometrial cancer remains unclear. The primary aim of this study was to analyze the prognostic significance of EP3 expression in endometrial cancer. We analyzed the EP3 expression of 140 endometrial carcinoma patients by immunohistochemistry. RL95-2 endometrial cancer cell line was chosen from four endometrial cancer cell lines (RL95-2, Ishikawa, HEC-1-A, and HEC-1-B) according to EP3 expression level. Treated with PGE2 and EP3 antagonist, RL95-2 cells were investigated by MTT, BrdU, and wound healing assay for functional assessment of EP3. EP3 staining differed significantly according to WHO tumor grading in both whole cohort (p = 0.01) and the subgroup of endometrioid carcinoma (p = 0.01). Patients with high EP3 expression in their respective tumors had impaired progression-free survival as well as overall survival in both cohorts above. EP3 expression in the overall cohort was identified as an independent prognostic marker for progression-free survival (HR 1.014, 95%CI 1.003-1.024, p = 0.01) when adjusted for age, stage, grading, and recurrence. Treatment with EP3 antagonists induced upregulation of estrogen receptor β and decreased activity of Ras and led to attenuated proliferation and migration of RL95-2 cells. EP3 seems to play a crucial role in endometrial cancer progression. In the context of limited systemic treatment options for endometrial cancer, this explorative analysis identifies EP3 as a potential target for diagnostic workup and therapy.

Security and skills: the two key issues in health worker migration

PubMed Central

Bidwell, Posy; Laxmikanth, Pallavi; Blacklock, Claire; Hayward, Gail; Willcox, Merlin; Peersman, Wim; Moosa, Shabir; Mant, David

2014-01-01

Background Migration of health workers from Africa continues to undermine the universal provision of quality health care. South Africa is an epicentre for migration – it exports more health workers to high-income countries than any other African country and imports health workers from its lower-income neighbours to fill the gap. Although an inter-governmental agreement in 2003 reduced the very high numbers migrating from South Africa to the United Kingdom, migration continues to other high-income English-speaking countries and few workers seem to return although the financial incentive to work abroad has lessened. A deeper understanding of reasons for migration from South Africa and post-migration experiences is therefore needed to underpin policy which is developed in order to improve retention within source countries and encourage return. Methods Semi-structured interviews were conducted with 16 South African doctors and nurses who had migrated to the United Kingdom. Interviews explored factors influencing the decision to migrate and post-migration experiences. Results Salary, career progression, and poor working conditions were not major push factors for migration. Many health workers reported that they had previously overcome these issues within the South African healthcare system by migrating to the private sector. Overwhelmingly, the major push factors were insecurity, high levels of crime, and racial tension. Although the wish to work and train in what was perceived to be a first-class care system was a pull factor to migrate to the United Kingdom, many were disappointed by the experience. Instead of obtaining new skills, many (particularly nurses) felt they had become ‘de-skilled’. Many also felt that working conditions and opportunities for them in the UK National Health Service (NHS) compared unfavourably with the private sector in South Africa. Conclusions Migration from South Africa seems unlikely to diminish until the major concerns over security, crime, and racial tensions are resolved. However, good working conditions in the private sector in South Africa provide an occupational incentive to return if security did improve. Potential migrants should be made more aware of the risks of losing skills while working abroad that might prejudice return. In addition, re-skilling initiatives should be encouraged. PMID:25079286

Security and skills: the two key issues in health worker migration.

PubMed

Bidwell, Posy; Laxmikanth, Pallavi; Blacklock, Claire; Hayward, Gail; Willcox, Merlin; Peersman, Wim; Moosa, Shabir; Mant, David

2014-01-01

Migration of health workers from Africa continues to undermine the universal provision of quality health care. South Africa is an epicentre for migration--it exports more health workers to high-income countries than any other African country and imports health workers from its lower-income neighbours to fill the gap. Although an inter-governmental agreement in 2003 reduced the very high numbers migrating from South Africa to the United Kingdom, migration continues to other high-income English-speaking countries and few workers seem to return although the financial incentive to work abroad has lessened. A deeper understanding of reasons for migration from South Africa and post-migration experiences is therefore needed to underpin policy which is developed in order to improve retention within source countries and encourage return. Semi-structured interviews were conducted with 16 South African doctors and nurses who had migrated to the United Kingdom. Interviews explored factors influencing the decision to migrate and post-migration experiences. Salary, career progression, and poor working conditions were not major push factors for migration. Many health workers reported that they had previously overcome these issues within the South African healthcare system by migrating to the private sector. Overwhelmingly, the major push factors were insecurity, high levels of crime, and racial tension. Although the wish to work and train in what was perceived to be a first-class care system was a pull factor to migrate to the United Kingdom, many were disappointed by the experience. Instead of obtaining new skills, many (particularly nurses) felt they had become 'de-skilled'. Many also felt that working conditions and opportunities for them in the UK National Health Service (NHS) compared unfavourably with the private sector in South Africa. Migration from South Africa seems unlikely to diminish until the major concerns over security, crime, and racial tensions are resolved. However, good working conditions in the private sector in South Africa provide an occupational incentive to return if security did improve. Potential migrants should be made more aware of the risks of losing skills while working abroad that might prejudice return. In addition, re-skilling initiatives should be encouraged.

Hippo kinases maintain polarity during directional cell migration in Caenorhabditis elegans.

PubMed

Feng, Guoxin; Zhu, Zhiwen; Li, Wen-Jun; Lin, Qirong; Chai, Yongping; Dong, Meng-Qiu; Ou, Guangshuo

2017-02-01

Precise positioning of cells is crucial for metazoan development. Despite immense progress in the elucidation of the attractive cues of cell migration, the repulsive mechanisms that prevent the formation of secondary leading edges remain less investigated. Here, we demonstrate that Caenorhabditis elegans Hippo kinases promote cell migration along the anterior-posterior body axis via the inhibition of dorsal-ventral (DV) migration. Ectopic DV polarization was also demonstrated in gain-of-function mutant animals for C. elegans RhoG MIG-2. We identified serine 139 of MIG-2 as a novel conserved Hippo kinase phosphorylation site and demonstrated that purified Hippo kinases directly phosphorylate MIG-2 S139 Live imaging analysis of genome-edited animals indicates that MIG-2 S139 phosphorylation impedes actin assembly in migrating cells. Intriguingly, Hippo kinases are excluded from the leading edge in wild-type cells, while MIG-2 loss induces uniform distribution of Hippo kinases. We provide evidence that Hippo kinases inhibit RhoG activity locally and are in turn restricted to the cell body by RhoG-mediated polarization. Therefore, we propose that the Hippo-RhoG feedback regulation maintains cell polarity during directional cell motility. © 2016 The Authors.

Hypoxia-inducible factor regulates alphavbeta3 integrin cell surface expression.

PubMed

Cowden Dahl, Karen D; Robertson, Sarah E; Weaver, Valerie M; Simon, M Celeste

2005-04-01

Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of alpha and beta aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt(-/-) and Hifalpha(-/-) TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin alphavbeta3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5% oxygen (O(2)). Culturing B16F0 melanoma cells at 1.5% O(2) resulted in increased alphavbeta3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O(2) tension influence placental invasion and tumor migration by increasing cell surface expression of alphavbeta3 integrin.

Hypoxia-inducible Factor Regulates αvβ3 Integrin Cell Surface Expression

PubMed Central

Cowden Dahl, Karen D.; Robertson, Sarah E.; Weaver, Valerie M.; Simon, M. Celeste

2005-01-01

Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of α and β aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt-/- and Hifα-/- TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin αvβ3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5% oxygen (O2). Culturing B16F0 melanoma cells at 1.5% O2 resulted in increased αvβ3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O2 tension influence placental invasion and tumor migration by increasing cell surface expression of αvβ3 integrin. PMID:15689487

Amplification or suppression: Social networks and the climate change—migration association in rural Mexico

PubMed Central

Riosmena, Fernando; Hunter, Lori M.; Runfola, Daniel M.

2015-01-01

Increasing rates of climate migration may be of economic and national concern to sending and destination countries. It has been argued that social networks – the ties connecting an origin and destination – may operate as “migration corridors” with the potential to strongly facilitate climate change-related migration. This study investigates whether social networks at the household and community levels amplify or suppress the impact of climate change on international migration from rural Mexico. A novel set of 15 climate change indices was generated based on daily temperature and precipitation data for 214 weather stations across Mexico. Employing geostatistical interpolation techniques, the climate change values were linked to 68 rural municipalities for which sociodemographic data and detailed migration histories were available from the Mexican Migration Project. Multi-level discrete-time event-history models were used to investigate the effect of climate change on international migration between 1986 and 1999. At the household level, the effect of social networks was approximated by comparing the first to the last move, assuming that through the first move a household establishes internal social capital. At the community level, the impact of social capital was explored through interactions with a measure of the proportion of adults with migration experience. The results show that rather than amplifying, social capital may suppress the sensitivity of migration to climate triggers, suggesting that social networks could facilitate climate change adaptation in place. PMID:26692656

Patterns in diurnal airspace use by migratory landbirds along an ecological barrier.

PubMed

Peterson, Anna C; Niemi, Gerald J; Johnson, Douglas H

2015-04-01

Migratory bird populations and survival are affected by conditions experienced during migration. While many studies and conservation and management efforts focus on terrestrial stoppage and staging areas, the aerial environment through which migrants move also is subjected to anthropogenic impacts with potential consequences to migratory movement and survival. During autumn migration, the northern coastline of Lake Superior acts as an ecological barrier for many landbirds migrating out of the boreal forests of North America. From 24 observation points, we assessed the diurnal movements of birds throughout autumn migration, 2008-2010, within a 210 × 10 km coastal region along the northern coast of Lake Superior. Several raptor species showed patterns in airspace associated with topographic features such as proximity to the coastline and presence of ridgelines. Funneling movement, commonly used to describe the concentration of raptors along a migratory diversion line that either prevents or enhances migration progress, occurred only for Bald and Golden Eagles. This suggests a "leaky" migration funnel for most migratory raptors (e.g., migrating birds exiting the purported migration corridor). Passerines migrating during the late season showed more spatial and temporal structure in airspace distribution than raptors did, including funneling and an association with airspace near the coast. We conclude that (1) the diurnal use of airspace by many migratory landbirds is patterned in space and time, (2) autumn count sites situated along ecological barriers substantially underestimate the number of raptors due to "leakage" out of these concentration areas, and (3) the magnitude and structure of diurnal passerine movements in airspace have been overlooked. The heavy and structured use of airspace by migratory landbirds, especially the airspace associated with anthropogenic development (e.g., buildings, towers, turbines) necessitates a shift in focus to airspace management and conservation attention for these animals.

Causes, trends, and policy of population migration and the floating population.

PubMed

Cai, F

1996-01-01

This study provides a discussion of migration theory, a description of the main characteristics of migrants and floating population in China, and a migration impact assessment and potential social policy directions. It is argued that the impetus for migration in China was the population distribution pattern and an uneven industrial structure that favored heavy industry. Another factor affecting migration is the gap in income between urban and rural areas, which has widened since reforms in the mid-1980s. The author finds the Todaro or the Harris-Todaro theories inappropriate for understanding migration in developing countries and flawed. Evidence about migrants' characteristics suggest that migration in China was part of a process governed by the laws of economic growth and market development. Legal migrants are defined as those who legally migrated according to the household registration system. China's development strategy during the 1950s relied on growth of capital-intensive heavy industry. The cost was underwritten by adoption of a price system that shifted the price of products in order to lower the cost of heavy industrial development. During 1952-78, agricultural output dropped sharply, but the employment structure changed very little and the rate of urbanization changed slightly. Regional inequality was obvious by 1978. As reform progressed, patterns emerged that favored eastern coastal development. Microlevel reform outdistanced macrolevel reform. Obstacles to migration were reduced: the breakup of the commune system and changes in the urban food supply system and housing. In 1990 there were 34.128 million migrants, of which 32.42% were interprovincial and 42.99% involved job-related shifts. In 1992, 8.1% of urban population were not registered; 94.909 million were floating population. Cities have established policies to match the size of the floating population to the current carrying capacity.

Patterns in diurnal airspace use by migratory landbirds along an ecological barrier

USGS Publications Warehouse

Peterson, Anna C.; Niemi, Gerald J.; Johnson, Douglas H.

2015-01-01

Migratory bird populations and survival are affected by conditions experienced during migration. While many studies and conservation and management efforts focus on terrestrial stoppage and staging areas, the aerial environment through which migrants move also is subjected to anthropogenic impacts with potential consequences to migratory movement and survival. During autumn migration, the northern coastline of Lake Superior acts as an ecological barrier for many landbirds migrating out of the boreal forests of North America. From 24 observation points, we assessed the diurnal movements of birds throughout autumn migration, 2008-2010, within a 210 km by 10 km coastal region along the northern coast of Lake Superior. Several raptor species showed patterns in airspace associated with topographic features such as proximity to the coastline and presence of ridgelines. Funneling movement, commonly used to describe the concentration of raptors along a migratory diversion line that either prevents or enhances migration progress, occurred only for Bald and Golden Eagles. This suggests a "leaky" migration funnel for most migratory raptors (e.g., migrating birds exiting the purported migration corridor). Passerines migrating during the late season showed more spatial and temporal structure in airspace distribution than raptors, including funneling and an association with airspace near the coast. We conclude that a) the diurnal use of airspace by many migratory landbirds is patterned in space and time, b) autumn count sites situated along ecological barriers substantially underestimate the number of raptors due to 'leakage' out of these concentration areas, and c) the magnitude and structure of diurnal passerine movements in airspace have been overlooked. The heavy and structured use of airspace by migratory landbirds, especially the airspace associated with anthropogenic development (e.g., buildings, towers, turbines) necessitates a shift in focus to airspace management and conservation attention for these animals.

Steelhead Supplementation in Idaho Rivers : 2001 Project Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Byrne, Alan

In 2001, Idaho Department of Fish and Game (IDFG) continued an assessment of the Sawtooth Hatchery steelhead Oncorhynchus mykiss stock to reestablish natural populations in Beaver and Frenchman creeks in the upper Salmon River. Crews stocked both streams with 20 pair of hatchery adults, and I estimated the potential smolt production from the 2000 adult outplants. n the Red River drainage, IDFG stocked Dworshak hatchery stock fingerlings and smolts from 1993 to 1999 to assess which life stage produces more progeny when the adults return to spawn. In 2001, IDFG operated the Red River weir to trap adults that returnedmore » from these stockings, but none were caught from either group. Wild steelhead populations in the Lochsa and Selway river drainages were assessed and the chinook salmon Oncorhynchus tshawytscha escapement was enumerated in Fish Creek. I estimated that 75 wild adult steelhead and 122 adult chinook salmon returned to Fish Creek in 2001. I estimated that slightly more than 30,000 juvenile steelhead migrated out of Fish Creek. This is the largest number of steelhead to migrate out of Fish Creek in a single year since I began estimating the yearly migration in 1994. Juvenile steelhead densities in Lochsa and Selway tributaries were somewhat higher in 2001 than those observed in 2000. Crews from IDFG collected over 4,800 fin samples from wild steelhead in 74 streams of the Clearwater, Snake, and Salmon river drainages and from five hatchery stocks during the summer of 2000 for a DNA analysis to assess Idaho's steelhead stock structure. The DNA analysis was subcontracted to Dr. Jennifer Nielsen, Alaska Biological Science Center, Anchorage. Her lab developed protocols to use for the analysis in 2001 and is continuing to analyze the samples. Dr. Nielsen plans to have the complete set of wild and hatchery stocks analyzed in 2002.« less

Climate shocks and rural-urban migration in Mexico: Exploring nonlinearities and thresholds.

PubMed

Nawrotzki, Raphael J; DeWaard, Jack; Bakhtsiyarava, Maryia; Ha, Jasmine Trang

2017-01-01

Adverse climatic conditions may differentially drive human migration patterns between rural and urban areas, with implications for changes in population composition and density, access to infrastructure and resources, and the delivery of essential goods and services. However, there is little empirical evidence to support this notion. In this study, we investigate the relationship between climate shocks and migration between rural and urban areas within Mexico. We combine individual records from the 2000 and 2010 Mexican censuses (n=683,518) with high-resolution climate data from Terra Populus that are linked to census data at the municipality level (n=2,321). We measure climate shocks as monthly deviation from a 30-year (1961-1990) long-term climate normal period, and uncover important nonlinearities using quadratic and cubic specifications. Satellite-based measures of urban extents allow us to classify migrant-sending and migrant-receiving municipalities as rural or urban to examine four internal migration patterns: rural-urban, rural-rural, urban-urban, and urban-rural. Among our key findings, results from multilevel models reveal that each additional drought month increases the odds of rural-urban migration by 3.6%. In contrast, the relationship between heat months and rural-urban migration is nonlinear. After a threshold of ~34 heat months is surpassed, the relationship between heat months and rural-urban migration becomes positive and progressively increases in strength. Policy and programmatic interventions may therefore reduce climate induced rural-urban migration in Mexico through rural climate change adaptation initiatives, while also assisting rural migrants in finding employment and housing in urban areas to offset population impacts.

Climate shocks and rural-urban migration in Mexico: Exploring nonlinearities and thresholds

PubMed Central

Nawrotzki, Raphael J.; DeWaard, Jack; Bakhtsiyarava, Maryia; Ha, Jasmine Trang

2016-01-01

Adverse climatic conditions may differentially drive human migration patterns between rural and urban areas, with implications for changes in population composition and density, access to infrastructure and resources, and the delivery of essential goods and services. However, there is little empirical evidence to support this notion. In this study, we investigate the relationship between climate shocks and migration between rural and urban areas within Mexico. We combine individual records from the 2000 and 2010 Mexican censuses (n=683,518) with high-resolution climate data from Terra Populus that are linked to census data at the municipality level (n=2,321). We measure climate shocks as monthly deviation from a 30-year (1961-1990) long-term climate normal period, and uncover important nonlinearities using quadratic and cubic specifications. Satellite-based measures of urban extents allow us to classify migrant-sending and migrant-receiving municipalities as rural or urban to examine four internal migration patterns: rural-urban, rural-rural, urban-urban, and urban-rural. Among our key findings, results from multilevel models reveal that each additional drought month increases the odds of rural-urban migration by 3.6%. In contrast, the relationship between heat months and rural-urban migration is nonlinear. After a threshold of ~34 heat months is surpassed, the relationship between heat months and rural-urban migration becomes positive and progressively increases in strength. Policy and programmatic interventions may therefore reduce climate induced rural-urban migration in Mexico through rural climate change adaptation initiatives, while also assisting rural migrants in finding employment and housing in urban areas to offset population impacts. PMID:28435176

FixO3 project results, legacy and module migration to EMSO

NASA Astrophysics Data System (ADS)

Lampitt, Richard

2017-04-01

The fixed point open ocean observatory network (FixO3) project is an international project aimed at integrating in a single network all fixed point open ocean observatories operated by European organisations and to harmonise and coordinate technological, procedural and data management across the stations. The project is running for four years since September 2013 with 29 partners across Europe and a budget of 7M Euros and is now coming to its final phase. In contrast to several past programmes, the opportunity has arisen to ensure that many of the project achievements can migrate into the newly formed European Multidisciplinary Seafloor and water column Observatory (EMSO) research infrastructure. The final phase of the project will focus on developing a strategy to transfer the results in an efficient way to maintain their relevance and maximise their use. In this presentation, we will highlight the significant achievements of FixO3 over the past three years focussing on the modules which will be transferred to EMSO in the coming 9 months. These include: 1. Handbook of best practices for operating fixed point observatories 2. Metadata catalogue 3. Earth Virtual Observatory (EarthVO) for data visualisation and comparison 4. Open Ocean Observatory Yellow Pages (O3YP) 5. Training material for hardware, data and data products used

MicroRNA-494-3p targets CXCR4 to suppress the proliferation, invasion, and migration of prostate cancer.

PubMed

Shen, Peng-fei; Chen, Xue-qin; Liao, Yong-chuan; Chen, Ni; Zhou, Qiao; Wei, Qiang; Li, Xiang; Wang, Jia; Zeng, Hao

2014-05-01

Although SDF-1/CXCR4 pathway is a potential mechanism of tumor proliferation and progression, the mechanism of controlling CXCR4 expression is not fully understood. This study was to confirm that miR-494-3p might be a potentially post-transcriptional regulator of CXCR4 and over-expression of miR-494 might suppress prostate cancer progression and metastasis. We firstly postulated the post-transcriptional regulation of CXCR4 by miR-494-3p through bioinformatics analysis, and then it was demonstrated that miR-494-3p could regulate the CXCR4 mRNA post-transcriptionally by binding to the predicted site by dual reporter gene assays. The biological effect of miR-494-3p on prostate cancer cells proliferation, apoptosis, migration, and invasion was measured by MTT, TUNEL, flow cytometry, migration, and invasion assays. It was shown that the mRNA and protein expression levels of CXCR4 were significantly up-regulated in PC-3 and DU145, whereas barely detected in LNCaP and RWPE-1. However, the CXCR4 protein levels were inversely related to the mature miR-494-3p expression levels in RWPE-1 and prostate cancer cells. The constitutive over-expression of miR-494-3p could down-regulate the protein level of CXCR4 in PC-3 and DU145. MiR-494-3p also could bind to the seed sequences in the 3'-UTR of the CXCR4 gene. Artificial over-expression of miR-494-3p could inhibit the growth, promote the apoptosis, and inhibit the migration and invasion of PC-3 and DU145 cells in vivo. Our results suggested that miR-494-3p might play crucial role in prostate cancer by post-transcriptional regulation to CXCR4 mRNA. MiR-494-3p/CXCR4 pathway may be a potential therapeutic target to prevent prostate cancer progression and metastasis. © 2014 Wiley Periodicals, Inc.

Cellular and molecular mechanisms of pomegranate juice-induced anti-metastatic effect on prostate cancer cells.

PubMed

Wang, Lei; Alcon, Andre; Yuan, Hongwei; Ho, Jeffrey; Li, Qi-Jing; Martins-Green, M

2011-07-01

Prostate cancer is the second leading cause of cancer-related deaths among US males. Pomegranate juice (PJ), a natural product, was shown in a clinical trial to inhibit progression of this disease. However, the underlying mechanisms involved in the anti-progression effects of PJ on prostate cancer remain unclear. Here we show that, in addition to causing cell death of hormone-refractory prostate cancer cells, PJ also increases cell adhesion and decreases cell migration of the cells that do not die. We hypothesized that PJ does so by stimulating the expression and/or activation of molecules that alter the cytoskeleton and the adhesion machinery of prostate cancer cells, resulting in enhanced cell adhesion and reduced cell migration. We took an integrative approach to these studies by using Affimetrix gene arrays to study gene expression, microRNA arrays to study the non-coding RNAs, molecules known to be disregulated in cancer cells, and Luminex Multiplex array assays to study the level of secreted pro-inflammatory cytokines/chemokines. PJ up-regulates genes involved in cell adhesion such as E-cadherin, intercellular adhesion molecule 1 (ICAM-1) and down-regulates genes involved in cell migration such as hyaluranan-mediated motility receptor (HMMR) and type I collagen. In addition, anti-invasive microRNAs such as miR-335, miR-205, miR-200, and miR-126, were up-regulated, whereas pro-invasive microRNA such as miR-21 and miR-373, were down-regulated. Moreover, PJ significantly reduced the level of secreted pro-inflammatory cytokines/chemokines such as IL-6, IL-12p40, IL-1β and RANTES, thereby having the potential to decrease inflammation and its impact on cancer progression. PJ also inhibits the ability of the chemokine SDF1α to chemoattract these cancer cells. SDF1α and its receptor CXCR4 are important in metastasis of cancer cells to the bone. Discovery of the mechanisms by which this enhanced adhesion and reduced migration are accomplished can lead to sophisticated and effective prevention of metastasis in prostate and potentially other cancers. This journal is © The Royal Society of Chemistry 2011

Soluble L1CAM promotes breast cancer cell adhesion and migration in vitro, but not invasion.

PubMed

Li, Yupei; Galileo, Deni S

2010-09-15

Neural recognition molecule L1CAM, which is a key protein involved in early nervous system development, is known to be abnormally expressed and shed in several types of cancers where it participates in metastasis and progression. The distinction of L1CAM presence in cancerous vs. normal tissues has suggested it to be a new target for cancer treatment. Our current study focused on the potential role of soluble L1CAM in breast cancer cell adhesion to extracellular matrix proteins, migration, and invasion. We found L1 expression levels were correlated with breast cancer stage of progression in established data sets of clinical samples, and also were high in more metastatic breast cancer cell lines MDA-MB-231 and MDA-MB-435, but low in less migratory MDA-MB-468 cells. Proteolysis of L1 into its soluble form (sL1) was detected in cell culture medium from all three above cell lines, and can be induced by PMA activation. Over-expression of the L1 ectodomain in MDA-MB-468 cells by using a lentiviral vector greatly increased the amount of sL1 released by those cells. Concomitantly, cell adhesion to extracellular matrix and cell transmigration ability were significantly promoted, while cell invasion ability through Matrigel™ remained unaffected. On the other hand, attenuating L1 expression in MDA-MB-231 cells by using a shRNA lentiviral vector resulted in reduced cell-matrix adhesion and transmigration. Similar effects were also shown by monoclonal antibody blocking of the L1 extracellular region. Moreover, sL1 in conditioned cell culture medium induced a directional migration of MDA-MB-468 cells, which could be neutralized by antibody treatment. Our data provides new evidence for the function of L1CAM and its soluble form in promoting cancer cell adhesion to ECM and cell migration. Thus, L1CAM is validated further to be a potential early diagnostic marker in breast cancer progression and a target for breast cancer therapy.

Soluble L1CAM promotes breast cancer cell adhesion and migration in vitro, but not invasion

PubMed Central

2010-01-01

Background Neural recognition molecule L1CAM, which is a key protein involved in early nervous system development, is known to be abnormally expressed and shed in several types of cancers where it participates in metastasis and progression. The distinction of L1CAM presence in cancerous vs. normal tissues has suggested it to be a new target for cancer treatment. Our current study focused on the potential role of soluble L1CAM in breast cancer cell adhesion to extracellular matrix proteins, migration, and invasion. Results We found L1 expression levels were correlated with breast cancer stage of progression in established data sets of clinical samples, and also were high in more metastatic breast cancer cell lines MDA-MB-231 and MDA-MB-435, but low in less migratory MDA-MB-468 cells. Proteolysis of L1 into its soluble form (sL1) was detected in cell culture medium from all three above cell lines, and can be induced by PMA activation. Over-expression of the L1 ectodomain in MDA-MB-468 cells by using a lentiviral vector greatly increased the amount of sL1 released by those cells. Concomitantly, cell adhesion to extracellular matrix and cell transmigration ability were significantly promoted, while cell invasion ability through Matrigel™ remained unaffected. On the other hand, attenuating L1 expression in MDA-MB-231 cells by using a shRNA lentiviral vector resulted in reduced cell-matrix adhesion and transmigration. Similar effects were also shown by monoclonal antibody blocking of the L1 extracellular region. Moreover, sL1 in conditioned cell culture medium induced a directional migration of MDA-MB-468 cells, which could be neutralized by antibody treatment. Conclusions Our data provides new evidence for the function of L1CAM and its soluble form in promoting cancer cell adhesion to ECM and cell migration. Thus, L1CAM is validated further to be a potential early diagnostic marker in breast cancer progression and a target for breast cancer therapy. PMID:20840789

Monitoring of Downstream Salmon and Steelhead at Federal Hydroelectric Facilities, 1991 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hawkes, Lynette A.; Martinson, Rick D.; Smith, W. William

1992-04-01

The 1991 smolt monitoring project of the National Marine Fisheries Service provided data on the seaward migration of juvenile salmon and steelhead at John Day, The Dalles and Bonneville Dams. All pertinent fish capture and condition data as well as dam operations and river flow data were provided to Fish Passage Center for use in developing fish passage indices and migration timing, and for water budget and spill management.

Derivation of persistent time for anisotropic migration of cells

NASA Astrophysics Data System (ADS)

Liu, Yan-Ping; Zhang, Xiao-Cui; Wu, Yu-Ling; Liu, Wen; Li, Xiang; Liu, Ru-Chuan; Liu, Li-Yu; Shuai, Jian-Wei

2017-12-01

Not Available Project supported by the National Natural Science Foundation of China (Grant Nos. 31370830, 11675134, 11474345, and 11604030), the State Key Development Program for Basic Research of China (Grant No. 2013CB837200), the 111 Project, China (Grant No. B16029), and the China Postdoctoral Science Foundation (Grant No. 2016M602071).

System and Campus Level Enrollment Projections and Environmental Scan, 1988-1998.

ERIC Educational Resources Information Center

Gaylord, Thomas A.; And Others

Alaska's first long-range, system-wide enrollment projection study since the inception of the higher education system's restructuring plan is reported. The study was undertaken in response to statewide economic distress, a decline in state funding of higher education, and a pattern of out-migration by the college-age population, particularly in…

Numerical modeling of dune progression in a high amplitude meandering channel

USDA-ARS?s Scientific Manuscript database

Laboratory experiments carried out by Abad and Garcia (2009) in a high-amplitude Kinoshita meandering channel show bed morphodynamics to comprise steady (local scour and deposition) and unsteady (migrating bedforms) components. The experiments are replicated with a numerical model. The sediment tran...

International migration beyond gravity: A statistical model for use in population projections

PubMed Central

Cohen, Joel E.; Roig, Marta; Reuman, Daniel C.; GoGwilt, Cai

2008-01-01

International migration will play an increasing role in the demographic future of most nations if fertility continues to decline globally. We developed an algorithm to project future numbers of international migrants from any country or region to any other. The proposed generalized linear model (GLM) used geographic and demographic independent variables only (the population and area of origins and destinations of migrants, the distance between origin and destination, the calendar year, and indicator variables to quantify nonrandom characteristics of individual countries). The dependent variable, yearly numbers of migrants, was quantified by 43653 reports from 11 countries of migration from 228 origins and to 195 destinations during 1960–2004. The final GLM based on all data was selected by the Bayesian information criterion. The number of migrants per year from origin to destination was proportional to (population of origin)0.86(area of origin)−0.21(population of destination)0.36(distance)−0.97, multiplied by functions of year and country-specific indicator variables. The number of emigrants from an origin depended on both its population and its population density. For a variable initial year and a fixed terminal year 2004, the parameter estimates appeared stable. Multiple R2, the fraction of variation in log numbers of migrants accounted for by the starting model, improved gradually with recentness of the data: R2 = 0.57 for data from 1960 to 2004, R2 = 0.59 for 1985–2004, R2 = 0.61 for 1995–2004, and R2 = 0.64 for 2000–2004. The migration estimates generated by the model may be embedded in deterministic or stochastic population projections. PMID:18824693

[Research progress of intervertebral disc endogenous stem cells for intervertebral disc regeneration].

PubMed

Liang, Hang; Deng, Xiangyu; Shao, Zengwu

2017-10-01

To summarize the research progress of intervertebral disc endogenous stem cells for intervertebral disc regeneration and deduce the therapeutic potential of endogenous repair for intervertebral disc degeneration. The original articles about intervertebral disc endogenous stem cells for intervertebral disc regeneration were extensively reviewed; the reparative potential in vivo and the extraction and identification in vitro of intervertebral disc endogenous stem cells were analyzed; the prospect of endogenous stem cells for intervertebral disc regeneration was predicted. Stem cell niche present in the intervertebral discs, from which stem cells migrate to injured tissues and contribute to tissues regeneration under certain specific microenvironment. Moreover, the migration of stem cells is regulated by chemokines system. Tissue specific progenitor cells have been identified and successfully extracted and isolated. The findings provide the basis for biological therapy of intervertebral disc endogenous stem cells. Intervertebral disc endogenous stem cells play a crucial role in intervertebral disc regeneration. Therapeutic strategy of intervertebral disc endogenous stem cells is proven to be a promising biological approach for intervertebral disc regeneration.

Multifaceted Leptin network: the molecular connection between obesity and breast cancer

PubMed Central

Saxena, Neeraj K.; Sharma, Dipali

2016-01-01

High plasma levels of leptin, a major adipocytokine produced by adipocytes, are correlated with increased fat mass in obese state. Leptin is emerging as a key candidate molecule linking obesity with breast cancer. Acting via endocrine, paracrine, and autocrine manner, leptin impacts various stages of breast tumorigenesis from initiation and primary tumor growth to metastatic progression. Leptin also modulates the tumor microenvironment mainly through supporting migration of endothelial cells, neo-angiogenesis and sustaining recruitment of macrophage and monocytes. Various studies have shown that hyperactive leptin-signaling network leads to concurrent activation of multiple oncogenic pathways resulting in enhanced proliferation, decreased apoptosis, acquisition of mesenchymal phenotype, potentiated migration and enhanced invasion potential of tumor cells. Furthermore, the capability of leptin to interact with other molecular effectors of obese state including, estrogen, IGF-1, insulin, VEGF and inflammatory cytokines further increases its impact on breast tumor progression in obese state. This article presents an overview of the studies investigating the involvement of leptin in breast cancer. PMID:24214584

SU-E-J-115: Using Markov Chain Modeling to Elucidate Patterns in Breast Cancer Metastasis Over Time and Space

DOE Office of Scientific and Technical Information (OSTI.GOV)

Comen, E; Mason, J; Kuhn, P

2014-06-01

Purpose: Traditionally, breast cancer metastasis is described as a process wherein cancer cells spread from the breast to multiple organ systems via hematogenous and lymphatic routes. Mapping organ specific patterns of cancer spread over time is essential to understanding metastatic progression. In order to better predict sites of metastases, here we demonstrate modeling of the patterned migration of metastasis. Methods: We reviewed the clinical history of 453 breast cancer patients from Memorial Sloan Kettering Cancer Center who were non-metastatic at diagnosis but developed metastasis over time. We used the variables of organ site of metastases as well as time tomore » create a Markov chain model of metastasis. We illustrate the probabilities of metastasis occurring at a given anatomic site together with the probability of spread to additional sites. Results: Based on the clinical histories of 453 breast cancer patients who developed metastasis, we have learned (i) how to create the Markov transition matrix governing the probabilities of cancer progression from site to site; (ii) how to create a systemic network diagram governing disease progression modeled as a random walk on a directed graph; (iii) how to classify metastatic sites as ‘sponges’ that tend to only receive cancer cells or ‘spreaders’ that receive and release them; (iv) how to model the time-scales of disease progression as a Weibull probability distribution function; (v) how to perform Monte Carlo simulations of disease progression; and (vi) how to interpret disease progression as an entropy-increasing stochastic process. Conclusion: Based on our modeling, metastatic spread may follow predictable pathways. Mapping metastasis not simply by organ site, but by function as either a ‘spreader’ or ‘sponge’ fundamentally reframes our understanding of metastatic processes. This model serves as a novel platform from which we may integrate the evolving genomic landscape that drives cancer metastasis. PS-OC Trans-Network Project Grant Award for “Data Assimilation and ensemble statistical forecasting methods applied to the MSKCC longitudinal metastatic breast cancer cohort.”.« less

Nectin-like molecule 1 inhibits the migration and invasion of U251 glioma cells by regulating the expression of an extracellular matrix protein osteopontin.

PubMed

Yin, Bin; Li, Ke-han; An, Tai; Chen, Tao; Peng, Xiao-zhong

2010-06-01

To investigate the molecular mechanism of nectin-like molecule 1 (NECL1) inhibiting the migration and invasion of U251 glioma cells. We infected U251 glioma cells with adeno-nectin-like molecule 1 (Ad-NECL1) or empty adenovirus (Ad). Transwell and wound healing assays were performed to observe the migration of U251 cells incubated with the cell supernatant from Ad-NECL1 or Ad infected U251 cells. DNA microarray was applied to screen the gene expression profile after the restoration of NECL1 in U251 glioma cell lines. The differential expression of osteopontin (OPN), a gene related to migration and invasion, was further analyzed with semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry. The restoration of NECL1 inhibited migration of U251 cells significantly (P<0.05). Altogether 195 genes were found differentially expressed by microarray, in which 175 were up-regulated and 20 down-regulated, including 9 extracellular matrix proteins involved in the migration of cells. Both mRNA and protein expressions of OPN, the most markedly reduced extracellular matrix protein, were found decreased in U251 cells after restoration of NECL1. Immunohistochemical assay also detected an increase of OPN in glioma tissues, related with the progressing of malignant grade. A link might exist between NECL1 and the extracellular matrix protein OPN in inhibiting the migration and invasion of U251 glioma cells.

Tornadic storm avoidance behavior in breeding songbirds.

PubMed

Streby, Henry M; Kramer, Gunnar R; Peterson, Sean M; Lehman, Justin A; Buehler, David A; Andersen, David E

2015-01-05

Migration is a common behavior used by animals of many taxa to occupy different habitats during different periods. Migrant birds are categorized as either facultative (i.e., those that are forced to migrate by some proximal cue, often weather) or obligate (i.e., those that migrate on a regular cycle). During migration, obligate migrants can curtail or delay flights in response to inclement weather or until favorable winds prevail, and they can temporarily reorient or reverse direction when ecological or meteorological obstacles are encountered. However, it is not known whether obligate migrants undertake facultative migrations and make large-scale movements in response to proximal cues outside of their regular migration periods. Here, we present the first documentation of obligate long-distance migrant birds undertaking a facultative migration, wherein breeding golden-winged warblers (Vermivora chrysoptera) carrying light-level geolocators performed a >1,500 km 5-day circumvention of a severe tornadic storm. The birds evacuated their breeding territories >24 hr before the arrival of the storm and atmospheric variation associated with it. The probable cue, radiating >1,000 km from tornadic storms, perceived by birds and influencing bird behavior and movements, is infrasound (i.e., sound below the range of human hearing). With the predicted increase in severity and frequency of similar storms as anthropogenic climate change progresses, understanding large-scale behavioral responses of animals to such events will be an important objective of future research. Copyright © 2015 Elsevier Ltd. All rights reserved.

A model for migration and accumulation of hydrocarbons in the Thamama and Arab reservoirs in Abu Dhabi, U.A.E.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hawas, M.F.; Takezaki, H.

1995-08-01

The distribution of hydrocarbons in the Lower Cretaceous Thamama Group and Upper Jurassic Arab Formation in Abu Dhabi is influenced by the development of the intervening Hith anhydrites. The geochemical analysis of Thamama and Arab hydrocarbons indicate that they were generated from a common source rock: the Upper Jurassic Diyab Formation. Studies carried out on the Miocene sabkha anhydrites in the coastal flat west of Abu Dhabi supported a model for vertical migration through the Hith anhydrites under certain conditions. The established model implies that the Diyab oil and gas had migrated essentially vertically and individually which means that themore » oil migrated prior to the gas and their distribution is controlled by the differential sealing potential of the anhydrites at each migration phase: a Hith anhydrite bed of more than 30 feet (ft.) thick was a perfect seal for hydrocarbon migration into the Arab reservoirs. In this case, oils could not break through to the overlying Thamama group. But where the anhydride bed thicknesses dropped below 30 ft. thick, this permitted oil migration through to the overlying Thamama reservoirs during the oil generation phase in the Turonian time. At a later stage, with additional depth of burial and progressive diagenesis anhydrite beds as thin as 8 ft. thick became effective seals. These controlled the distribution of the gas during the gas generation phase in the Eocene time.« less

Weather conditions associated with autumn migration by mule deer in Wyoming.

PubMed

Rittenhouse, Chadwick D; Mong, Tony W; Hart, Thomas

2015-01-01

Maintaining ecological integrity necessitates a proactive approach of identifying and acquiring lands to conserve unfragmented landscapes, as well as evaluating existing mitigation strategies to increase connectivity in fragmented landscapes. The increased use of highway underpasses and overpasses to restore connectivity for wildlife species offers clear conservation benefits, yet also presents a unique opportunity to understand how weather conditions may impact movement of wildlife species. We used remote camera observations (19,480) from an existing wildlife highway underpass in Wyoming and daily meteorological observations to quantify weather conditions associated with autumn migration of mule deer in 2009 and 2010. We identified minimal daily temperature and snow depth as proximate cues associated with mule deer migration to winter range. These weather cues were consistent across does and bucks, but differed slightly by year. Additionally, extreme early season snow depth or cold temperature events appear to be associated with onset of migration. This information will assist wildlife managers and transportation officials as they plan future projects to maintain and enhance migration routes for mule deer.

Final Project Memorandum: Ecological implications of mangrove forest migration in the southeastern U.S.

USGS Publications Warehouse

Osland, Michael J.; Day, Richard H.; Krauss, Ken W.; From, Andrew S.; Larriviere, Jack C.; Hester, Mark W.; Yando, Erik S.; Willis, Jonathan A

2014-01-01

Winter climate change has the potential to have a large impact on coastal wetlands in the southeastern United States. Warmer winter temperatures and reductions in the intensity of freeze events would likely lead to mangrove forest range expansion and salt marsh displacement in parts of the U.S. Gulf of Mexico and Atlantic coast. The objective of this research was to better evaluate the ecological implications of mangrove forest migration and salt marsh displacement. The potential ecological impacts of mangrove migration are diverse ranging from important biotic impacts (e.g., coastal fisheries, land bird migration; colonial-nesting wading birds) to ecosystem stability (e.g., response to sea level rise and drought; habitat loss; coastal protection) to biogeochemical processes (e.g., carbon storage; water quality). This research specifically investigated the impact of mangrove forest migration on coastal wetland soil processes and the consequent implications for coastal wetland responses to sea level rise and carbon storage.

Migration, business formation, and the informal economy in urban Mexico.

PubMed

Sheehan, Connor M; Riosmena, Fernando

2013-07-01

Although the informal economy has grown rapidly in several developing nations, and migration and informality may be related to similar types of credit constraints and market failures, previous research has not systematically attempted to identify if migrant households are more likely to start informal and formal businesses alike and if this association varies across local contexts. We examine the relationship between prior US migration and the creation of both formal and informal businesses in urban Mexico using several criteria to indirectly assess sector location. We use data from 56 communities from the Mexican Migration Project to estimate multilevel survival and nonmultilevel competing risk models predicting the likelihood of informal, formal, and no business formation. The recent return migration of the household head is strongly associated with informal business creation, particularly in economically dynamic areas. On the other hand, migrants are only marginally more likely to start formal businesses in highly economically dynamic sending areas. Published by Elsevier Inc.

Migration from new-accession countries and duration expectancy in the EU-15: 2002–2008

PubMed Central

DeWaard, Jack; Ha, Jasmine Trang; Raymer, James; Wiśniowski, Arkadiusz

2016-01-01

European Union (EU) enlargements in 2004 and 2007 were accompanied by increased migration from new-accession to established-member (EU-15) countries. The impacts of these flows depend, in part, on the amount of time that persons from the former countries live in the latter over the life course. In this paper, we develop period estimates of duration expectancy in EU-15 countries among persons from new-accession countries. Using a newly developed set of harmonised Bayesian estimates of migration flows each year from 2002 to 2008 from the Integrated Modelling of European Migration (IMEM) Project, we exploit period age patterns of country-to-country migration and mortality to summarize the average number of years that persons from new-accession countries could be expected to live in EU-15 countries over the life course. In general, the results show that the amount of time that persons from new-accession countries could be expected to live in the EU-15 nearly doubled after 2004. PMID:28286353

News & Views Evaluation of Projects for Basic Research of Scientific Instruments in 2008 Completed NSFC Former President Tang Ao-qing Passed Away Professor Tang Aoqing and NSFC Carbon Nanotube Film-Based Speaker Developed in Tsinghua University Dinosaur Footprint Fossils Discovered in Xinjiang New Method for Early Cancer Diagnosis New Progress Achieved by NSFC Project in Basic Research of Black Hole Physics New progress in Organic FET 67 NSFC-RFBR Cooperative Projects Approved for 2008 Spin Configuration and Super-exchange Mechanism in Molecular Magnets Observed NSFC Strengthens its Funding in Wenchuan Concerns Go to Disaster's Impact on Economy and Emergency Response Thirty-seven NSFC-KOSEF Cooperative Projects Approved

NASA Astrophysics Data System (ADS)

2008-01-01

Evaluation of Projects for Basic Research of Scientific Instruments in 2008 Completed NSFC Former President Tang Ao-qing Passed Away Professor Tang Aoqing and NSFC Carbon Nanotube Film-Based Speaker Developed in Tsinghua University Dinosaur Footprint Fossils Discovered in Xinjiang New Method for Early Cancer Diagnosis New Progress Achieved by NSFC Project in Basic Research of Black Hole Physics New progress in Organic FET 67 NSFC-RFBR Cooperative Projects Approved for 2008 Spin Configuration and Super-exchange Mechanism in Molecular Magnets Observed NSFC Strengthens its Funding in Wenchuan Concerns Go to Disaster's Impact on Economy and Emergency Response Thirty-seven NSFC-KOSEF Cooperative Projects Approved

Increased Flight Altitudes among Migrating Golden Eagles Suggest Turbine Avoidance at a Rocky Mountain Wind Installation

PubMed Central

Johnston, Naira N.; Bradley, James E.; Otter, Ken A.

2014-01-01

Potential wind-energy development in the eastern Rocky Mountain foothills of British Columbia, Canada, raises concerns due to its overlap with a golden eagle (Aquila chrysaetos) migration corridor. The Dokie 1 Wind Energy Project is the first development in this area and stands as a model for other projects in the area because of regional consistency in topographic orientation and weather patterns. We visually tracked golden eagles over three fall migration seasons (2009–2011), one pre- and two post-construction, to document eagle flight behaviour in relation to a ridge-top wind energy development. We estimated three-dimensional positions of eagles in space as they migrated through our study site. Flight tracks were then incorporated into GIS to ascertain flight altitudes for eagles that flew over the ridge-top area (or turbine string). Individual flight paths were designated to a category of collision-risk based on flight altitude (e.g. flights within rotor-swept height; ≤150 m above ground) and wind speed (winds sufficient for the spinning of turbines; >6.8 km/h at ground level). Eagles were less likely to fly over the ridge-top area within rotor-swept height (risk zone) as wind speed increased, but were more likely to make such crosses under headwinds and tailwinds compared to western crosswinds. Most importantly, we observed a smaller proportion of flights within the risk zone at wind speeds sufficient for the spinning of turbines (higher-risk flights) during post-construction compared to pre-construction, suggesting that eagles showed detection and avoidance of turbines during migration. PMID:24671199

Increased flight altitudes among migrating golden eagles suggest turbine avoidance at a Rocky Mountain wind installation.

PubMed

Johnston, Naira N; Bradley, James E; Otter, Ken A

2014-01-01

Potential wind-energy development in the eastern Rocky Mountain foothills of British Columbia, Canada, raises concerns due to its overlap with a golden eagle (Aquila chrysaetos) migration corridor. The Dokie 1 Wind Energy Project is the first development in this area and stands as a model for other projects in the area because of regional consistency in topographic orientation and weather patterns. We visually tracked golden eagles over three fall migration seasons (2009-2011), one pre- and two post-construction, to document eagle flight behaviour in relation to a ridge-top wind energy development. We estimated three-dimensional positions of eagles in space as they migrated through our study site. Flight tracks were then incorporated into GIS to ascertain flight altitudes for eagles that flew over the ridge-top area (or turbine string). Individual flight paths were designated to a category of collision-risk based on flight altitude (e.g. flights within rotor-swept height; ≤150 m above ground) and wind speed (winds sufficient for the spinning of turbines; >6.8 km/h at ground level). Eagles were less likely to fly over the ridge-top area within rotor-swept height (risk zone) as wind speed increased, but were more likely to make such crosses under headwinds and tailwinds compared to western crosswinds. Most importantly, we observed a smaller proportion of flights within the risk zone at wind speeds sufficient for the spinning of turbines (higher-risk flights) during post-construction compared to pre-construction, suggesting that eagles showed detection and avoidance of turbines during migration.

Sustaining control: lessons from the Lubombo spatial development initiative in southern Africa.

PubMed

Maharaj, Rajendra; Moonasar, Devanand; Baltazar, Candrinho; Kunene, Simon; Morris, Natashia

2016-08-12

The Lubombo Spatial Development Initiative (LSDI) was a tri-country project between South Africa, Swaziland and Mozambique with the aim of accelerating socio-economic development in the region. The malaria component of the project was introduced to decrease the transmission of malaria in the region. This goal was met but with termination of this project resulted in an upsurge of malaria cases in the sub-region mainly as a result of migration from high transmission areas to low transmission ones. The movement of people across borders in southern Africa remains a challenge in sustaining malaria control and elimination. Malaria case data for Swaziland and South Africa were obtained from their respective national Malaria Information Systems. Data for Mozambique was obtained from the Mozambican Ministry of Health. Data obtained during the course of the LSDI project was compared to the case data post the termination of the LSDI. The 12-year period of the LSDI showed a substantial decrease in disease burden amongst the three countries involved when compared to the baseline year of 2000. The decrease in malaria cases was 99 % in South Africa and 98 % in Swaziland. Malaria prevalence in Mozambique decreased by 85 % over the same period. However, after the LSDI ended, between 2012 and 2014, there was an upward trend in case data that was counter to the goal of elimination. South Africa and Swaziland benefitted from the LSDI and were able to sustain malaria control and progress to the stage of elimination. Mozambique could not sustain the gains made during the LSDI and case numbers increased. Technical and financial resources are key challenges for malaria control and elimination interventions.

Human renal adipose tissue induces the invasion and progression of renal cell carcinoma.

PubMed

Campo-Verde-Arbocco, Fiorella; López-Laur, José D; Romeo, Leonardo R; Giorlando, Noelia; Bruna, Flavia A; Contador, David E; López-Fontana, Gastón; Santiano, Flavia E; Sasso, Corina V; Zyla, Leila E; López-Fontana, Constanza M; Calvo, Juan C; Carón, Rubén W; Creydt, Virginia Pistone

2017-11-07

We evaluated the effects of conditioned media (CMs) of human adipose tissue from renal cell carcinoma located near the tumor (hRATnT) or farther away from the tumor (hRATfT), on proliferation, adhesion and migration of tumor (786-O and ACHN) and non-tumor (HK-2) human renal epithelial cell lines. Human adipose tissues were obtained from patients with renal cell carcinoma (RCC) and CMs from hRATnT and hRATfT incubation. Proliferation, adhesion and migration were quantified in 786-O, ACHN and HK-2 cell lines incubated with hRATnT-, hRATfT- or control-CMs. We evaluated versican, adiponectin and leptin expression in CMs from hRATnT and hRATfT. We evaluated AdipoR1/2, ObR, pERK, pAkt y pPI3K expression on cell lines incubated with CMs. No differences in proliferation of cell lines was found after 24 h of treatment with CMs. All cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRATnT-CMs vs. hRATfT- or control-CMs. hRATnT-CMs showed increased levels of versican and leptin, compared to hRATfT-CMs. AdipoR2 in 786-O and ACHN cells decreased significantly after incubation with hRATfT- and hRATnT-CMs vs. control-CMs. We observed a decrease in the expression of pAkt in HK-2, 786-O and ACHN incubated with hRATnT-CMs. This result could partially explain the observed changes in migration and cell adhesion. We conclude that hRATnT released factors, such as leptin and versican, could enhance the invasive potential of renal epithelial cell lines and could modulate the progression of the disease.

Interleukin-23 promotes the migration and invasion of gastric cancer cells by inducing epithelial-to-mesenchymal transition via the STAT3 pathway.

PubMed

Xu, Xianhui; Yang, Changdong; Chen, Jun; Liu, Junyan; Li, Ping'ang; Shi, Yan; Yu, Peiwu

2018-05-05

Chronic inflammation is associated with all stages of cancer development. Moreover, a proinflammatory microenvironment resulted from chronic inflammation is considered to be an essential component of cancer. Interleukin-23 (IL-23) is a general proinflammatory factor; and is involved in tumor-associated inflammation in gastric cancer (GC). However, the direct effect of IL-23 on GC cells has been rarely reported. The aim of the study was to clarify the direct role of IL-23 in regulating GC progression, and to identify the underlying mechanism. In this study, Positive expression of IL-23R was observed in GC tissues and cell lines by using immunohistochemistry or immunofluorescence. In western blots, the expression of IL-23R was higher in GC tissues compared with adjacent normal tissues. Furthermore, IL-23R positive GC tissues were closely related with larger tumor size and worse T stage and clinical stage. By performing in vitro experiments, we found that IL-23 binding to its receptor promoted the migration and invasion of BGC-823 cells in vitro. Moreover, IL-23 induced the activation of STAT3 and epithelial-to-mesenchymal transition (EMT) in BGC-823 cells. Knocking down STAT3 in BGC-823 cells attenuated the effect of IL-23 on EMT and cell migration and invasion. Taken together, our study has firstly demonstrated the positive expression of IL-23R in human GC tissues and cell lines. IL-23 binding to its receptor promotes the migration and invasion of GC cells by inducing EMT through the STAT3 signaling pathway. This work provides a new mechanism for the oncogenic role of IL-23 on GC progression. Copyright © 2018. Published by Elsevier Inc.

Expression of breast cancer metastasis suppressor-1, BRMS-1, in human breast cancer and the biological impact of BRMS-1 on the migration of breast cancer cells.

PubMed

Zhang, Yulu; Ye, Lin; Tan, Yuxia; Sun, Pinghui; Ji, Ke; Jiang, Wen G

2014-03-01

Breast cancer metastasis suppressor-1 (BRMS1) is a candidate metastasis-suppressing gene and has been shown to potentially inhibit tumor progression without blocking the growth of orthotopic tumors, in different tumor types including non-small cell lung cancer, ovarian, melanoma and breast cancers. BRMS-1 gene transcript was quantified in breast cancer sample tissues and analyzed against histological and clinical patient outcome. Human breast cancer cell lines, MDA MB-231 and MCF-7 were used to genetically-modify the expression of BRMS-1 and test for biological responses following BRMS-1 modifications. Key candidate signal pathways, influenced by BRMS-1 were also explored. BRMS1 was present in MDA MB-231 and MCF-7 cell lines. Using anti-BRMS1 transgenes, we knocked-down the transcripts of BRMS1 in both cells at the mRNA and protein levels. Knockdown of BRMS1 gave both cells a faster cell growth rate, rapid pace of cellular migration and invasion, compared to respective wild-type and control cells (p<0.05). Blocking phospholipase-Cγ (PLCγ) had a significant influence on the BRMS-1-induced cell migration. Finally, significantly low levels of BRMS1 were observed in patients with high-grade tumors (p=0.12), in patients with distant metastasis (p=0.05) and those who died of breast cancer (p=0.0037). In addition, patients with low levels of BRMS1 had a significantly shorter overall survival (p=0.035). BRMS-1 is aberrantly expressed in human breast cancer and is inversely-correlated with disease progression and patient survival. This is likely to be occurring via its influence on invasion and migration of breast cancer cells.

Knockdown of Uba2 inhibits colorectal cancer cell invasion and migration through downregulation of the Wnt/β-catenin signaling pathway.

PubMed

Cheng, Hongjing; Sun, Xun; Li, Ji; He, Ping; Liu, Wanqi; Meng, Xiangwei

2018-05-10

Colorectal cancer is a serious threat to human health, and has a high mortality rate. There is currently no effective therapy for end-stage colorectal cancer. In recent years, molecular targeted therapy has received increasing attention for cancer treatment. In particular, the role of Uba2, a vital component of SUMO-activating enzyme, has been highlighted, which plays important roles in the progression of certain cancers; however, its role in colorectal cancer remains unclear. Accordingly, the aim of this study was to evaluate the relationship between Uba2 and colorectal cancer. Uba2 expression was knocked down in two colorectal cancer cell lines, and gene microarray analysis was conducted, followed by proliferation, migration, and invasion assays. Uba2 knockdown influenced the expression of several genes, and significantly inhibited the proliferation, migration, and invasion of cancer cells. To determine the underlying mechanism, the expression of related signaling pathways and molecules was evaluated in the knockdown cell lines. Overall, the results suggest that Uba2 participates in the progression, invasion, and metastasis of colorectal cancer, and the possible mechanism is via regulating the Wnt signaling pathway and enhancing epithelial-mesenchymal transition behaviors of colorectal cancer cells. Therefore, Uba2 is expected to be an important oncoprotein and potential therapeutic target in colorectal cancer. © 2018 Wiley Periodicals, Inc.

Surface Modeling to Support Small-Body Spacecraft Exploration and Proximity Operations

NASA Technical Reports Server (NTRS)

Riedel, Joseph E.; Mastrodemos, Nickolaos; Gaskell, Robert W.

2011-01-01

In order to simulate physically plausible surfaces that represent geologically evolved surfaces, demonstrating demanding surface-relative guidance navigation and control (GN&C) actions, such surfaces must be made to mimic the geological processes themselves. A report describes how, using software and algorithms to model body surfaces as a series of digital terrain maps, a series of processes was put in place that evolve the surface from some assumed nominal starting condition. The physical processes modeled in this algorithmic technique include fractal regolith substrate texturing, fractally textured rocks (of empirically derived size and distribution power laws), cratering, and regolith migration under potential energy gradient. Starting with a global model that may be determined observationally or created ad hoc, the surface evolution is begun. First, material of some assumed strength is layered on the global model in a fractally random pattern. Then, rocks are distributed according to power laws measured on the Moon. Cratering then takes place in a temporal fashion, including modeling of ejecta blankets and taking into account the gravity of the object (which determines how much of the ejecta blanket falls back to the surface), and causing the observed phenomena of older craters being progressively buried by the ejecta of earlier impacts. Finally, regolith migration occurs which stratifies finer materials from coarser, as the fine material progressively migrates to regions of lower potential energy.

Role of cationic channel TRPV2 in promoting prostate cancer migration and progression to androgen resistance.

PubMed

Monet, Michaël; Lehen'kyi, V'yacheslav; Gackiere, Florian; Firlej, Virginie; Vandenberghe, Matthieu; Roudbaraki, Morad; Gkika, Dimitra; Pourtier, Albin; Bidaux, Gabriel; Slomianny, Christian; Delcourt, Philippe; Rassendren, François; Bergerat, Jean-Pierre; Ceraline, Jocelyn; Cabon, Florence; Humez, Sandrine; Prevarskaya, Natalia

2010-02-01

Castration resistance in prostate cancer (PCa) constitutes an advanced, aggressive disease with poor prognosis, associated with uncontrolled cell proliferation, resistance to apoptosis, and enhanced invasive potential. The molecular mechanisms involved in the transition of PCa to castration resistance are obscure. Here, we report that the nonselective cationic channel transient receptor potential vanilloid 2 (TRPV2) is a distinctive feature of castration-resistant PCa. TRPV2 transcript levels were higher in patients with metastatic cancer (stage M1) compared with primary solid tumors (stages T2a and T2b). Previous studies of the TRPV2 channel indicated that it is primarily involved in cancer cell migration and not in cell growth. Introducing TRPV2 into androgen-dependent LNCaP cells enhanced cell migration along with expression of invasion markers matrix metalloproteinase (MMP) 9 and cathepsin B. Consistent with the likelihood that TRPV2 may affect cancer cell aggressiveness by influencing basal intracellular calcium levels, small interfering RNA-mediated silencing of TRPV2 reduced the growth and invasive properties of PC3 prostate tumors established in nude mice xenografts, and diminished expression of invasive enzymes MMP2, MMP9, and cathepsin B. Our findings establish a role for TRPV2 in PCa progression to the aggressive castration-resistant stage, prompting evaluation of TRPV2 as a potential prognostic marker and therapeutic target in the setting of advanced PCa.

APPL1-Mediating Leptin Signaling Contributes to Proliferation and Migration of Cancer Cells.

PubMed

Ding, Youming; Cao, Yingkang; Wang, Bin; Wang, Lei; Zhang, Yemin; Zhang, Deling; Chen, Xiaoyan; Li, Mingxin; Wang, Changhua

2016-01-01

Leptin has been implicated in tumorigenesis and tumor progression, particularly in obese patients. As a multifunctional adaptor protein, APPL1 (containing pleckstrin homology domain, phosphotyrosine binding domain, and a leucine zipper motif 1) plays a critical role in regulating adiponectin and insulin signaling pathways. Currently, high APPL1 level has been suggested to be related to metastases and progression of some types of cancer. However, the intercourse between leptin signaling pathway and APPL1 remains poorly understood. Here, we show that the protein levels and phosphorylation statues of APPL1were highly expressed in tissues from human hepatocellular carcinoma and triple-positive breast cancer. Leptin stimulated APPL1 phosphorylation in a time-dependent manner in both human hepatocellular carcinoma HepG2 cell and breast cancer MCF-7 cell. Overexpression or suppression of APPL1 promoted or attenuated, respectively, leptin-induced phosphorylation of STAT3, ERK1/2, and Akt in the cancer cells, accompanied with enhanced or mitigated cell proliferation and migration. In addition, we identified that APPL1 directly bound to both leptin receptor and STAT3. This interaction was significantly enhanced by leptin stimulation. Our results suggested that APPL1 positively mediated leptin signaling and promoted leptin-induced proliferation and migration of cancer cells. This finding reveals a novel mechanism by which leptin promotes the motility and growth of cancer cells.

Signal regulatory protein α associated with the progression of oral leukoplakia and oral squamous cell carcinoma regulates phenotype switch of macrophages.

PubMed

Ye, Xiaojing; Zhang, Jing; Lu, Rui; Zhou, Gang

2016-12-06

Signal regulatory protein α (SIRPα) is a cell-surface protein expressed on macrophages that are regarded as an important component of the tumor microenvironment. The expression of SIRPα in oral leukoplakia (OLK) and oral squamous cell carcinoma (OSCC), and further explored the role of SIRPα on the phenotype, phagocytosis ability, migration, and invasion of macrophages in OSCC were investigated. The expression of SIRPα in OLK was higher than in OSCC, correlating with the expression of CD68 and CD163 on macrophages. After cultured with the conditioned media of oral cancer cells, the expression of SIRPα on THP-1 cells was decreased gradually. In co-culture system, macrophages were induced into M2 phenotype by oral cancer cells. Blockade of SIRPα inhibited phagocytosis ability and IL-6, TNF-α productions of macrophages. In addition, the proliferation, migration, and IL-10, TGF-β productions of macrophages were upregulated after blockade of SIRPα. Macrophages upregulated the expression of SIRPα and phagocytosis ability, and inhibited the migration and invasion when the activation of NF-κB was inhibited by pyrrolidine dithiocarbamate ammonium (PDTC). Hence, SIRPα might play an important role in the progression of OLK and oral cancer, and could be a pivotal therapeutic target in OSCC by regulating the phenotype of macrophages via targeting NF-κB.

Progranulin expression in advanced human atherosclerotic plaque.

PubMed

Kojima, Yoji; Ono, Koh; Inoue, Katsumi; Takagi, Yasushi; Kikuta, Ken-ichiro; Nishimura, Masaki; Yoshida, Yoshinori; Nakashima, Yasuhiro; Matsumae, Hironobu; Furukawa, Yutaka; Mikuni, Nobuhiro; Nobuyoshi, Masakiyo; Kimura, Takeshi; Kita, Toru; Tanaka, Makoto

2009-09-01

Progranulin (PGRN) is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. However, when it is degraded to granulin peptides (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. Since injury, inflammation and repair are common features in the progression of atherosclerosis, it is conceivable that PGRN plays a role in atherogenesis. Immunohistochemical analysis of human carotid endoatherectomy specimens indicated that vascular smooth muscle cells (vSMCs) in the intima expressed PGRN. Some macrophages in the plaque also expressed PGRN. We assessed the effect of PGRN on a human monocytic leukemia cell line (THP-1) and human aortic smooth muscle cells (HASMCs). PGRN alone had no effect on HASMC or THP-1 proliferation or migration. However, when THP-1 cells were stimulated with MCP-1, the number of migrated cells decreased in a PGRN-dose-dependent manner. TNF-alpha-induced HASMC migration was enhanced only at 10nM of PGRN. Interleukin-8 (IL-8) secretion from HASMCs was reduced by forced expression of PGRN and increased by RNAi-mediated knockdown of PGRN. While exogenous treatment with recombinant PGRN decreased IL-8 secretion, degraded recombinant GRNs increased IL-8 secretion from HASMCs. The expression of PGRN mainly reduces inflammation and its degradation into GRNs enhances inflammation in atherosclerotic plaque and may contribute to the progression of atherosclerosis.

Knockdown of long noncoding RNA CCAT1 inhibits cell growth, invasion and peritoneal metastasis via downregulation of Bmi-1 in gastric cancer.

PubMed

Li, N; Jiang, K; Fang, L P; Yao, L L; Yu, Z

2018-06-26

Long noncoding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in gastric cancer (GC) tissues compared with normal counterparts and CCAT1 upregulation can promote proliferation and migration of GC cells in vitro. B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) expression is positively correlated with tumor progression. The present study aimed to investigate the biological functions of CCAT1 and the relationships between CCAT1 and Bmi-1 in GC progression. In the present study, CCAT1 was knocked down by specific shRNA transfection in two human GC cell lines (MGC-803 and SGC-7901). The effects of CCAT1 knockdown on GC cell proliferation, cell cycle, migration and invasion were investigated in vitro. The effect of CCAT1 knockdown on peritoneal metastasis was assessed in nude mice. Bmi-1 expression levels were examined both in vitro and in vivo. The results showed that CCAT1 knockdown markedly inhibited cell proliferation, migration and invasion, arrested the cell cycle at G0/G1 phase in vitro, and inhibited peritoneal metastasis in nude mice, along with the downregulation of Bmi-1. Taken together, CCAT1 is functionally involved in growth and metastasis of GC cells and it may be a potential target for GC therapy.

Pleiotrophin exerts its migration and invasion effect through the neuropilin-1 pathway.

PubMed

Elahouel, Rania; Blanc, Charly; Carpentier, Gilles; Frechault, Sophie; Cascone, Ilaria; Destouches, Damien; Delbé, Jean; Courty, José; Hamma-Kourbali, Yamina

2015-08-01

Pleiotrophin (PTN) is a pleiotropic growth factor that exhibits angiogenic properties and is involved in tumor growth and metastasis. Although it has been shown that PTN is expressed in tumor cells, few studies have investigated its receptors and their involvement in cell migration and invasion. Neuropilin-1 (NRP-1) is a receptor for multiple growth factors that mediates cell motility and plays an important role in angiogenesis and tumor progression. Here we provide evidence for the first time that NRP-1 is crucial for biological activities of PTN. We found that PTN interacted directly with NRP-1 through its thrombospondin type-I repeat domains. Importantly, binding of PTN to NRP-1 stimulated the internalization and recycling of NRP-1 at the cell surface. Invalidation of NRP-1 by RNA interference in human carcinoma cells inhibited PTN-induced intracellular signaling of the serine-threonine kinase, mitogen-activated protein MAP kinase, and focal adhesion kinase pathways. Accordingly, NRP-1 silencing or blocking by antibody inhibited PTN-induced human umbilical vein endothelial cell migration and tumor cell invasion. These results suggest that NRP-1/PTN interaction provides a novel mechanism for controlling the response of endothelial and tumoral cells to PTN and may explain, at least in part, how PTN contributes to tumor angiogenesis and cancer progression. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Multiple intracerebroventricular injections of human umbilical cord mesenchymal stem cells delay motor neurons loss but not disease progression of SOD1G93A mice.

PubMed

Sironi, Francesca; Vallarola, Antonio; Violatto, Martina Bruna; Talamini, Laura; Freschi, Mattia; De Gioia, Roberta; Capelli, Chiara; Agostini, Azzurra; Moscatelli, Davide; Tortarolo, Massimo; Bigini, Paolo; Introna, Martino; Bendotti, Caterina

2017-12-01

Stem cell therapy is considered a promising approach in the treatment of amyotrophic lateral sclerosis (ALS) and mesenchymal stem cells (MSCs) seem to be the most effective in ALS animal models. The umbilical cord (UC) is a source of highly proliferating fetal MSCs, more easily collectable than other MSCs. Recently we demonstrated that human (h) UC-MSCs, double labeled with fluorescent nanoparticles and Hoechst-33258 and transplanted intracerebroventricularly (ICV) into SOD1G93A transgenic mice, partially migrated into the spinal cord after a single injection. This prompted us to assess the effect of repeated ICV injections of hUC-MSCs on disease progression in SOD1G93A mice. Although no transplanted cells migrated to the spinal cord, a partial but significant protection of motor neurons (MNs) was found in the lumbar spinal cord of hUC-MSCs-treated SOD1G93A mice, accompanied by a shift from a pro-inflammatory (IL-6, IL-1β) to anti-inflammatory (IL-4, IL-10) and neuroprotective (IGF-1) environment in the lumbar spinal cord, probably linked to the activation of p-Akt survival pathway in both motor neurons and reactive astrocytes. However, this treatment neither prevented the muscle denervation nor delayed the disease progression of mice, emphasizing the growing evidence that protecting the motor neuron perikarya is not sufficient to delay the ALS progression. Copyright © 2017. Published by Elsevier B.V.

Forecasting of Radiation Belts: Results From the PROGRESS Project.

NASA Astrophysics Data System (ADS)

Balikhin, M. A.; Arber, T. D.; Ganushkina, N. Y.; Walker, S. N.

2017-12-01

Forecasting of Radiation Belts: Results from the PROGRESS Project. The overall goal of the PROGRESS project, funded in frame of EU Horizon2020 programme, is to combine first principles based models with the systems science methodologies to achieve reliable forecasts of the geo-space particle radiation environment.The PROGRESS incorporates three themes : The propagation of the solar wind to L1, Forecast of geomagnetic indices, and forecast of fluxes of energetic electrons within the magnetosphere. One of the important aspects of the PROGRESS project is the development of statistical wave models for magnetospheric waves that affect the dynamics of energetic electrons such as lower band chorus, hiss and equatorial noise. The error reduction ratio (ERR) concept has been used to optimise the set of solar wind and geomagnetic parameters for organisation of statistical wave models for these emissions. The resulting sets of parameters and statistical wave models will be presented and discussed. However the ERR analysis also indicates that the combination of solar wind and geomagnetic parameters accounts for only part of the variance of the emissions under investigation (lower band chorus, hiss and equatorial noise). In addition, advances in the forecast of fluxes of energetic electrons, exploiting empirical models and the first principles IMPTAM model achieved by the PROGRESS project is presented.

MeCP2 overexpression inhibits proliferation, migration and invasion of C6 glioma by modulating ERK signaling and gene expression.

PubMed

Sharma, Kedarlal; Singh, Juhi; Frost, Emma E; Pillai, Prakash P

2018-05-01

MethylCpG binding protein-2 (MeCP2) is an epigenetic regulator and essential for brain development. MeCP2 mutations are associated with a spectrum of neuro-developmental disorders that vary depending on the patient gender, most notably Rett Syndrome. MeCP2 is essential for normal neuronal maturation, and glial cell function in the brain. Besides, its role in neurodevelopmental disorders, MeCP2 is involved in many cancers such as breast, colorectal, lung, liver, and prostate cancer. Glioma is the most lethal form of brain cancer. Studies have shown that dysfunctional epigenetic regulation plays a crucial role in glioma progression. Further, previous studies have suggested a role for MeCP2 in glioma pathogenesis. In this study, we show that MeCP2 may play a critical role in the suppression of glioma progression. Stable overexpression of MeCP2in C6 glioma cells inhibits proliferation, migration, invasion, and adhesion. Moreover, MeCP2 overexpression inhibits pERKand BDNF expression while inducing GFAP expression in C6 glioma. These findings suggest that MeCP2 may play a crucial role in suppression of glioma progression. Copyright © 2018 Elsevier B.V. All rights reserved.

Monocarboxylate transporter 1 contributes to growth factor-induced tumor cell migration independent of transporter activity

PubMed Central

Gray, Alana L.; Coleman, David T.; Shi, Runhua; Cardelli, James A.

2016-01-01

Tumor progression to metastatic disease contributes to the vast majority of incurable cancer. Understanding the processes leading to advanced stage cancer is important for the development of future therapeutic strategies. Here, we establish a connection between tumor cell migration, a prerequisite to metastasis, and monocarboxylate transporter 1 (MCT1). MCT1 transporter activity is known to regulate aspects of tumor progression and, as such, is a clinically relevant target for treating cancer. Knockdown of MCT1 expression caused decreased hepatocyte growth factor (HGF)-induced as well as epidermal growth factor (EGF)-induced tumor cell scattering and wound healing. Western blot analysis suggested that MCT1 knockdown (KD) hinders signaling through the HGF receptor (c-Met) but not the EGF receptor. Exogenous, membrane-permeable MCT1 substrates were not able to rescue motility in MCT1 KD cells, nor was pharmacologic inhibition of MCT1 able to recapitulate decreased cell motility as seen with MCT1 KD cells, indicating transporter activity of MCT1 was dispensable for EGF- and HGF-induced motility. These results indicate MCT1 expression, independent of transporter activity, is required for growth factor-induced tumor cell motility. The findings presented herein suggest a novel function for MCT1 in tumor progression independent of its role as a monocarboxylate transporter. PMID:27127175

Exosomes from human colorectal cancer induce a tumor-like behavior in colonic mesenchymal stromal cells

PubMed Central

Lugini, Luana; Valtieri, Mauro; Federici, Cristina; Cecchetti, Serena; Meschini, Stefania; Condello, Maria; Signore, Michele; Fais, Stefano

2016-01-01

Background Cancer cells, including colorectal cancer ones (CRC), release high amounts of nanovesicles (exosomes), delivering biochemical messages for paracrine or systemic crosstalk. Mesenchymal stromal cells (MSCs) have been shown to play contradicting roles in tumor progression. Results CRC exosomes induce in cMSCs: i) atypical morphology, higher proliferation, migration and invasion; ii) formation of spheroids; iii) an acidic extracellular environment associated with iv) a plasma membrane redistribution of vacuolar H+-ATPase and increased expression of CEA. Colon cancer derived MSCs, which were isolated from tumor masses, produce umbilicated spheroids, a future frequently observed in the inner core of rapidly growing tumors and recapitulate the changes observed in normal colonic MSCs exposed to CRC exosomes. Materials and Methods Tissue specific colonic (c)MSCs were exposed to primary or metastatic CRC exosomes and analysed by light and electron microscopy, proliferation in 2D and 3D cultures, migration and invasion assays, Western blot and confocal microscopy for vacuolar H+-ATPase expression. Conclusions CRC exosomes are able to induce morphological and functional changes in colonic MSCs, which may favour tumor growth and its malignant progression. Our results suggest that exosomes are actively involved in cancer progression and that inhibiting tumor exosome release may represent a way to interfere with cancer. PMID:27418137

Exosomes from human colorectal cancer induce a tumor-like behavior in colonic mesenchymal stromal cells.

PubMed

Lugini, Luana; Valtieri, Mauro; Federici, Cristina; Cecchetti, Serena; Meschini, Stefania; Condello, Maria; Signore, Michele; Fais, Stefano

2016-08-02

Cancer cells, including colorectal cancer ones (CRC), release high amounts of nanovesicles (exosomes), delivering biochemical messages for paracrine or systemic crosstalk. Mesenchymal stromal cells (MSCs) have been shown to play contradicting roles in tumor progression. CRC exosomes induce in cMSCs: i) atypical morphology, higher proliferation, migration and invasion; ii) formation of spheroids; iii) an acidic extracellular environment associated with iv) a plasma membrane redistribution of vacuolar H+-ATPase and increased expression of CEA. Colon cancer derived MSCs, which were isolated from tumor masses, produce umbilicated spheroids, a future frequently observed in the inner core of rapidly growing tumors and recapitulate the changes observed in normal colonic MSCs exposed to CRC exosomes. Tissue specific colonic (c)MSCs were exposed to primary or metastatic CRC exosomes and analysed by light and electron microscopy, proliferation in 2D and 3D cultures, migration and invasion assays, Western blot and confocal microscopy for vacuolar H+-ATPase expression. CRC exosomes are able to induce morphological and functional changes in colonic MSCs, which may favour tumor growth and its malignant progression. Our results suggest that exosomes are actively involved in cancer progression and that inhibiting tumor exosome release may represent a way to interfere with cancer.

miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gao, Yong, E-mail: gaoyongunion@163.com; Luo, Ling-hui; Li, Shuai

2014-02-07

Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensinmore » homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.« less

Macrophage/epithelium cross-talk regulates cell cycle progression and migration in pancreatic progenitors.

PubMed

Mussar, Kristin; Tucker, Andrew; McLennan, Linsey; Gearhart, Addie; Jimenez-Caliani, Antonio J; Cirulli, Vincenzo; Crisa, Laura

2014-01-01

Macrophages populate the mesenchymal compartment of all organs during embryogenesis and have been shown to support tissue organogenesis and regeneration by regulating remodeling of the extracellular microenvironment. Whether this mesenchymal component can also dictate select developmental decisions in epithelia is unknown. Here, using the embryonic pancreatic epithelium as model system, we show that macrophages drive the epithelium to execute two developmentally important choices, i.e. the exit from cell cycle and the acquisition of a migratory phenotype. We demonstrate that these developmental decisions are effectively imparted by macrophages activated toward an M2 fetal-like functional state, and involve modulation of the adhesion receptor NCAM and an uncommon "paired-less" isoform of the transcription factor PAX6 in the epithelium. Over-expression of this PAX6 variant in pancreatic epithelia controls both cell motility and cell cycle progression in a gene-dosage dependent fashion. Importantly, induction of these phenotypes in embryonic pancreatic transplants by M2 macrophages in vivo is associated with an increased frequency of endocrine-committed cells emerging from ductal progenitor pools. These results identify M2 macrophages as key effectors capable of coordinating epithelial cell cycle withdrawal and cell migration, two events critical to pancreatic progenitors' delamination and progression toward their differentiated fates.

The limits to cumulative causation: international migration from Mexican urban areas.

PubMed

Fussell, Elizabeth; Massey, Douglas S

2004-02-01

We present theoretical arguments and empirical research to suggest that the principal mechanisms of cumulative causation do not function in large urban settings. Using data from the Mexican Migration Project, we found evidence of cumulative causation in small cities, rural towns and villages, but not in large urban areas. With event-history models, we found little positive effect of community-level social capital and a strong deterrent effect of urban labor markets on the likelihood of first and later U.S. trips for residents of urban areas in Mexico, suggesting that the social process of migration from urban areas is distinct from that in the more widely studied rural migrant-sending communities of Mexico.

Overcoming the Problems of Inconsistent International Migration data: A New Method Applied to Flows in Europe

PubMed Central

Raymer, James; van der Erf, Rob; van Wissen, Leo

2010-01-01

Due to differences in definitions and measurement methods, cross-country comparisons of international migration patterns are difficult and confusing. Emigration numbers reported by sending countries tend to differ from the corresponding immigration numbers reported by receiving countries. In this paper, a methodology is presented to achieve harmonised estimates of migration flows benchmarked to a specific definition of duration. This methodology accounts for both differences in definitions and the effects of measurement error due to, for example, under reporting and sampling fluctuations. More specifically, the differences between the two sets of reported data are overcome by estimating a set of adjustment factors for each country’s immigration and emigration data. The adjusted data take into account any special cases where the origin–destination patterns do not match the overall patterns. The new method for harmonising migration flows that we present is based on earlier efforts by Poulain (European Journal of Population, 9(4): 353–381 1993, Working Paper 12, joint ECE-Eurostat Work Session on Migration Statistics, Geneva, Switzerland 1999) and is illustrated for movements between 19 European countries from 2002 to 2007. The results represent a reliable and consistent set of international migration flows that can be used for understanding recent changes in migration patterns, as inputs into population projections and for developing evidence-based migration policies. PMID:21124647

Overcoming the Problems of Inconsistent International Migration data: A New Method Applied to Flows in Europe.

PubMed

de Beer, Joop; Raymer, James; van der Erf, Rob; van Wissen, Leo

2010-11-01

Due to differences in definitions and measurement methods, cross-country comparisons of international migration patterns are difficult and confusing. Emigration numbers reported by sending countries tend to differ from the corresponding immigration numbers reported by receiving countries. In this paper, a methodology is presented to achieve harmonised estimates of migration flows benchmarked to a specific definition of duration. This methodology accounts for both differences in definitions and the effects of measurement error due to, for example, under reporting and sampling fluctuations. More specifically, the differences between the two sets of reported data are overcome by estimating a set of adjustment factors for each country's immigration and emigration data. The adjusted data take into account any special cases where the origin-destination patterns do not match the overall patterns. The new method for harmonising migration flows that we present is based on earlier efforts by Poulain (European Journal of Population, 9(4): 353-381 1993, Working Paper 12, joint ECE-Eurostat Work Session on Migration Statistics, Geneva, Switzerland 1999) and is illustrated for movements between 19 European countries from 2002 to 2007. The results represent a reliable and consistent set of international migration flows that can be used for understanding recent changes in migration patterns, as inputs into population projections and for developing evidence-based migration policies.

Application of Geographic Information System (GIS) to Model the Hydrocarbon Migration: Case Study from North-East Malay Basin, Malaysia

NASA Astrophysics Data System (ADS)

Rudini; Nasir Matori, Abd; Talib, Jasmi Ab; Balogun, Abdul-Lateef

2018-03-01

The purpose of this study is to model the migration of hydrocarbon using Geographic Information System (GIS). Understanding hydrocarbon migration is important since it can mean the difference between success and failure in oil and gas exploration project. The hydrocarbon migration modeling using geophysical method is still not accurate due to the limitations of available data. In recent years, GIS has emerged as a powerful tool for subsurface mapping and analysis. Recent studies have been carried out about the abilities of GIS to model hydrocarbon migration. Recent advances in GIS support the establishment and monitoring of prediction hydrocarbon migration. The concept, model, and calculation are based on the current geological situation. The spatial data of hydrocarbon reservoirs is determined by its geometry of lithology and geophysical attributes. Top of Group E horizon of north-east Malay basin was selected as the study area due to the occurrence of hydrocarbon migration. Spatial data and attributes data such as seismic data, wells log data and lithology were acquired and processed. Digital Elevation Model (DEM) was constructed from the selected horizon as a result of seismic interpretation using the Petrel software. Furthermore, DEM was processed in ArcGIS as a base map to shown hydrocarbon migration in north-east Malay Basin. Finally, all the data layers were overlaid to produce a map of hydrocarbon migration. A good data was imported to verify the model is correct.

β4-Integrin/PI3K Signaling Promotes Tumor Progression through the Galectin-3-N-Glycan Complex.

PubMed

Kariya, Yukiko; Oyama, Midori; Hashimoto, Yasuhiro; Gu, Jianguo; Kariya, Yoshinobu

2018-06-01

Malignant transformation is associated with aberrant N -glycosylation, but the role of protein N -glycosylation in cancer progression remains poorly defined. β4-integrin is a major carrier of N -glycans and is associated with poor prognosis, tumorigenesis, and metastasis. Here, N -glycosylation of β4-integrin contributes to the activation of signaling pathways that promote β4-dependent tumor development and progression. Increased expression of β1,6GlcNAc-branched N -glycans was found to be colocalized with β4-integrin in human cutaneous squamous cell carcinoma tissues, and that the β1,6GlcNAc residue was abundant on β4-integrin in transformed keratinocytes. Interruption of β1,6GlcNAc-branching formation on β4-integrin with the introduction of bisecting GlcNAc by N -acetylglucosaminyltransferase III overexpression was correlated with suppression of cancer cell migration and tumorigenesis. N -Glycan deletion on β4-integrin impaired β4-dependent cancer cell migration, invasion, and growth in vitro and diminished tumorigenesis and proliferation in vivo The reduced abilities of β4-integrin were accompanied with decreased phosphoinositol-3 kinase (PI3K)/Akt signals and were restored by the overexpression of the constitutively active p110 PI3K subunit. Binding of galectin-3 to β4-integrin via β1,6GlcNAc-branched N -glycans promoted β4-integrin-mediated cancer cell adhesion and migration. In contrast, a neutralizing antibody against galectin-3 attenuated β4-integrin N -glycan-mediated PI3K activation and inhibited the ability of β4-integrin to promote cell motility. Furthermore, galectin-3 knockdown by shRNA suppressed β4-integrin N -glycan-mediated tumorigenesis. These findings provide a novel role for N -glycosylation of β4-integrin in tumor development and progression, and the regulatory mechanism for β4-integrin/PI3K signaling via the galectin-3- N -glycan complex. Implications: N -Glycosylation of β4-integrin plays a functional role in promoting tumor development and progression through PI3K activation via the galectin-3- N -glycan complex. Mol Cancer Res; 16(6); 1024-34. ©2018 AACR . ©2018 American Association for Cancer Research.

The influence of age-specific migration on housing growth in the rural Midwest (USA)

Treesearch

Yohan Lee; Claire A. Montgomery; Jeff Kline

2016-01-01

Natural resource policymakers and planners increasingly rely on regional and national-level spatial data describing projections of future housing growth, to anticipate development impacts on natural resources and identify policy and planning needs. Such projections have not always been well-grounded in demographic and other factors that influence population and thus...

A Longitudinal Analysis of Family Migration and the Gender Gap in Earnings in the United States and Great Britain

PubMed Central

COOKE, THOMAS J.; BOYLE, PAUL; COUCH, KENNETH; FEIJTEN, PETEKE

2009-01-01

This article uses longitudinal data for the United States and Great Britain to examine the impact of residential mobility and childbirth on the earnings of women, their family earnings, and the related division of earnings by gender. This project is the first to compare explicitly the impact of childbirth and family migration on women’s earnings, and it extends prior cross-sectional and longitudinal studies on isolated countries by providing a direct contrast between two major industrialized nations, using comparable measures. The results indicate that families respond in similar ways in both countries to migration and childbirth. In response to both migration and childbirth, women’s earnings fall at the time of the event and recover slowly afterward, but the magnitude of the impact is roughly twice as large for childbirth as for migration. However, migration—but not the birth of a child—is also associated with a significant increase in total family earnings because of increased husbands’ earnings. As a result, the effect of migration on the relative earnings of wives to husbands is similar to the effect of childbirth. These results suggest that family migration should be given consideration in the literature on the gender earnings gap. PMID:19348113

An expert panel process to evaluate habitat restoration actions in the Columbia River estuary.

PubMed

Krueger, Kirk L; Bottom, Daniel L; Hood, W Gregory; Johnson, Gary E; Jones, Kim K; Thom, Ronald M

2017-03-01

We describe a process for evaluating proposed ecosystem restoration projects intended to improve survival of juvenile salmon in the Columbia River estuary (CRE). Changes in the Columbia River basin (northwestern USA), including hydropower development, have contributed to the listing of 13 salmon stocks as endangered or threatened under the U.S. Endangered Species Act. Habitat restoration in the CRE, from Bonneville Dam to the ocean, is part of a basin-wide, legally mandated effort to mitigate federal hydropower impacts on salmon survival. An Expert Regional Technical Group (ERTG) was established in 2009 to improve and implement a process for assessing and assigning "survival benefit units" (SBUs) to restoration actions. The SBU concept assumes site-specific restoration projects will increase juvenile salmon survival during migration through the 234 km CRE. Assigned SBUs are used to inform selection of restoration projects and gauge mitigation progress. The ERTG standardized the SBU assessment process to improve its scientific integrity, repeatability, and transparency. In lieu of experimental data to quantify the survival benefits of individual restoration actions, the ERTG adopted a conceptual model composed of three assessment criteria-certainty of success, fish opportunity improvements, and habitat capacity improvements-to evaluate restoration projects. Based on these criteria, an algorithm assigned SBUs by integrating potential fish density as an indicator of salmon performance. Between 2009 and 2014, the ERTG assessed SBUs for 55 proposed projects involving a total of 181 restoration actions located across 8 of 9 reaches of the CRE, largely relying on information provided in a project template based on the conceptual model, presentations, discussions with project sponsors, and site visits. Most projects restored tidal inundation to emergent wetlands, improved riparian function, and removed invasive vegetation. The scientific relationship of geomorphic and salmonid responses to restoration actions remains the foremost concern. Although not designed to establish a broad strategy for estuary restoration, the scoring process has adaptively influenced the types, designs, and locations of restoration proposals. The ERTG process may be a useful model for others who have unique ecosystem restoration goals and share some of our common challenges. Copyright © 2016 Elsevier Ltd. All rights reserved.

A family affair: A Ral-exocyst-centered network links Ras, Rac, Rho signaling to control cell migration.

PubMed

Zago, Giulia; Biondini, Marco; Camonis, Jacques; Parrini, Maria Carla

2017-05-12

Cell migration is central to many developmental, physiologic and pathological processes, including cancer progression. The Ral GTPases (RalA and RalB) which act down-stream the Ras oncogenes, are key players in the coordination between membrane trafficking and actin polymerization. A major direct effector of Ral, the exocyst complex, works in polarized exocytosis and is at the center of multiple protein-protein interactions that support cell migration by promoting protrusion formation, front-rear polarization, and extra-cellular matrix degradation. In this review we describe the recent advancements in deciphering the molecular mechanisms underlying this role of Ral via exocyst on cell migration. Among others, we will discuss the recently identified cross-talk between Ral and Rac1 pathways: exocyst binds to a negative regulator (the RacGAP SH3BP1) and to the major effector (the Wave Regulatory Complex, WRC) of Rac1, the master regulator of protrusions. Next challenge will be to better characterize the dynamics in space and in time of these molecular interplays, to better understand the pleiotropic functions of Ral in both normal and cancer cells.

ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion

PubMed Central

Chen, Wei-Ta; Ebelt, Nancy D; Stracker, Travis H; Xhemalce, Blerta; Van Den Berg, Carla L; Miller, Kyle M

2015-01-01

Ataxia-telangiectasia mutated (ATM) protein kinase regulates the DNA damage response (DDR) and is associated with cancer suppression. Here we report a cancer-promoting role for ATM. ATM depletion in metastatic cancer cells reduced cell migration and invasion. Transcription analyses identified a gene network, including the chemokine IL-8, regulated by ATM. IL-8 expression required ATM and was regulated by oxidative stress. IL-8 was validated as an ATM target by its ability to rescue cell migration and invasion defects in ATM-depleted cells. Finally, ATM-depletion in human breast cancer cells reduced lung tumors in a mouse xenograft model and clinical data validated IL-8 in lung metastasis. These findings provide insights into how ATM activation by oxidative stress regulates IL-8 to sustain cell migration and invasion in cancer cells to promote metastatic potential. Thus, in addition to well-established roles in tumor suppression, these findings identify a role for ATM in tumor progression. DOI: http://dx.doi.org/10.7554/eLife.07270.001 PMID:26030852

Learning by Doing: the Progressive Novella Project.

ERIC Educational Resources Information Center

Conroy, Michael G.

1996-01-01

States that the Progressive Novella Project for high school students involves the collaborative writing of a 35-50 page novella. Explains that prior to the actual writing process, students are educated in the basic elements of fiction writing. Describes the division of labor into groups. Comments that the results of the project are invariably…

Progress Report 18 for the Period February to July 1981 and Proceeidngs of the 18th Project Integration Meeting

NASA Technical Reports Server (NTRS)

1981-01-01

Progress in the low cost solar array project during the period February to July 1981 is reported. Included are: (1) project analysis and integration; (2) technology development in silicon material, large area silicon sheer and encapsulation; (3) process development; (4) engineering, and operations.

Extracellular Acidic pH Inhibits Oligodendrocyte Precursor Viability, Migration, and Differentiation

PubMed Central

Jagielska, Anna; Wilhite, Kristen D.; Van Vliet, Krystyn J.

2013-01-01

Axon remyelination in the central nervous system requires oligodendrocytes that produce myelin. Failure of this repair process is characteristic of neurodegeneration in demyelinating diseases such as multiple sclerosis, and it remains unclear how the lesion microenvironment contributes to decreased remyelination potential of oligodendrocytes. Here, we show that acidic extracellular pH, which is characteristic of demyelinating lesions, decreases the migration, proliferation, and survival of oligodendrocyte precursor cells (OPCs), and reduces their differentiation into oligodendrocytes. Further, OPCs exhibit directional migration along pH gradients toward acidic pH. These in vitro findings support a possible in vivo scenario whereby pH gradients attract OPCs toward acidic lesions, but resulting reduction in OPC survival and motility in acid decreases progress toward demyelinated axons and is further compounded by decreased differentiation into myelin-producing oligodendrocytes. As these processes are integral to OPC response to nerve demyelination, our results suggest that lesion acidity could contribute to decreased remyelination. PMID:24098762

The "Yin" and "Yang" of Cell Cycle Progression and Differentiation in the Oligodendroglial Lineage

ERIC Educational Resources Information Center

Nguyen, Laurent; Borgs, Laurence; Vandenbosch, Renaud; Mangin, Jean-Marie; Beukelaers, Pierre; Moonen, Gustave; Gallo, Vittorio; Malgrange, Brigitte; Belachew, Shibeshih

2006-01-01

In white matter disorders such as leukodystrophies (LD), periventricular leucomalacia (PVL), or multiple sclerosis (MS), the hypomyelination or the remyelination failure by oligodendrocyte progenitor cells involves errors in the sequence of events that normally occur during development when progenitors proliferate, migrate through the white…

"Lesbian Migrants in the Gentrified Valley" and "Other" Geographies of Rural Gentrification

ERIC Educational Resources Information Center

Smith, Darren P.; Holt, Louise

2005-01-01

This paper explores the migration and cultural consumption practices of lesbian households within processes of rural change. Taking forward Phillips' (2004. Progress in Human Geography 28, 5-30) discussion of neglected geographies of rural gentrification, and building upon Halfacree's (2001. International Journal of Population Geography 7,…

AS Migration and Optimization of the Power Integrated Data Network

NASA Astrophysics Data System (ADS)

Zhou, Junjie; Ke, Yue

2018-03-01

In the transformation process of data integration network, the impact on the business has always been the most important reference factor to measure the quality of network transformation. With the importance of the data network carrying business, we must put forward specific design proposals during the transformation, and conduct a large number of demonstration and practice to ensure that the transformation program meets the requirements of the enterprise data network. This paper mainly demonstrates the scheme of over-migrating point-to-point access equipment in the reconstruction project of power data comprehensive network to migrate the BGP autonomous domain to the specified domain defined in the industrial standard, and to smooth the intranet OSPF protocol Migration into ISIS agreement. Through the optimization design, eventually making electric power data network performance was improved on traffic forwarding, traffic forwarding path optimized, extensibility, get larger, lower risk of potential loop, the network stability was improved, and operational cost savings, etc.

FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis.

PubMed

Knüppel, Larissa; Heinzelmann, Katharina; Lindner, Michael; Hatz, Rudolf; Behr, Jürgen; Eickelberg, Oliver; Staab-Weijnitz, Claudia A

2018-04-19

In idiopathic pulmonary fibrosis (IPF), fibroblasts gain a more migratory phenotype and excessively secrete extracellular matrix (ECM), ultimately leading to alveolar scarring and progressive dyspnea. Here, we analyzed the effects of deficiency of FK506-binding protein 10 (FKBP10), a potential IPF drug target, on primary human lung fibroblast (phLF) adhesion and migration. Using siRNA, FKBP10 expression was inhibited in phLF in absence or presence of 2ng/ml transforming growth factor-β1 (TGF-β1) and 0.1mM 2-phosphoascorbate. Effects on cell adhesion and migration were monitored by an immunofluorescence (IF)-based attachment assay, a conventional scratch assay, and single cell tracking by time-lapse microscopy. Effects on expression of key players in adhesion dynamics and migration were analyzed by qPCR and Western Blot. Colocalization was evaluated by IF microscopy and by proximity ligation assays. FKBP10 knockdown significantly attenuated adhesion and migration of phLF. Expression of collagen VI was decreased, while expression of key components of the focal adhesion complex was mostly upregulated. The effects on migration were 2-phosphoascorbate-dependent, suggesting collagen synthesis as the underlying mechanism. FKBP10 colocalized with collagen VI and coating culture dishes with collagen VI, and to a lesser extent with collagen I, abolished the effect of FKBP10 deficiency on migration. These findings show, to our knowledge for the first time, that FKBP10 interacts with collagen VI and that deficiency of FKBP10 reduces phLF migration mainly by downregulation of collagen VI synthesis. The results strengthen FKBP10 as an important intracellular regulator of ECM remodeling and support the concept of FKBP10 as drug target in IPF.

Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells.

PubMed

Yan, Lin-Lin; Huang, Yuan-Jiao; Yi, Xiang; Yan, Xue-Min; Cai, Yan; He, Qin; Han, Zi-Jian

2015-06-01

The calcium-binding S100 proteins are involved in functions such as cell growth, differentiation, migration, adhesion and signal transduction. S100A8 and S100A9 are highly expressed in a variety of tumor cells, and are implicated in tumor development and progression. However, the role of S100A8 and S100A9 in nasopharyngeal carcinoma (NPC) cell migration is unclear. The present study investigated the effect of S100A8 and S100A9 on migration using a NPC cell line, CNE1. The CNE1 cells were transfected with S100A8 or S100A9 small interfering RNA (siRNA). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect S100A8 and S100A9 gene expression. Following the downregulation of S100A8 or S100A9, the effects on cell migration were determined using wound-healing assays. The expression of matrix metalloproteinase-7 (MMP7), a member of the MMP family that is associated with tumor cell invasion and migration, was also detected by RT-qPCR. S100A8 and S100A9 siRNAs effectively suppressed S100A8 and S100A9 gene expression, and substantially inhibited the migration of the CNE1 cells. In addition, MMP7 expression was reduced to varying extents in S100A8 and S100A9 siRNA-treated cells compared with controls. Thus, S100A8 and S100A9 promoted the migration of CNE1 NPC cells.

Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells

PubMed Central

YAN, LIN-LIN; HUANG, YUAN-JIAO; YI, XIANG; YAN, XUE-MIN; CAI, YAN; HE, QIN; HAN, ZI-JIAN

2015-01-01

The calcium-binding S100 proteins are involved in functions such as cell growth, differentiation, migration, adhesion and signal transduction. S100A8 and S100A9 are highly expressed in a variety of tumor cells, and are implicated in tumor development and progression. However, the role of S100A8 and S100A9 in nasopharyngeal carcinoma (NPC) cell migration is unclear. The present study investigated the effect of S100A8 and S100A9 on migration using a NPC cell line, CNE1. The CNE1 cells were transfected with S100A8 or S100A9 small interfering RNA (siRNA). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect S100A8 and S100A9 gene expression. Following the downregulation of S100A8 or S100A9, the effects on cell migration were determined using wound-healing assays. The expression of matrix metalloproteinase-7 (MMP7), a member of the MMP family that is associated with tumor cell invasion and migration, was also detected by RT-qPCR. S100A8 and S100A9 siRNAs effectively suppressed S100A8 and S100A9 gene expression, and substantially inhibited the migration of the CNE1 cells. In addition, MMP7 expression was reduced to varying extents in S100A8 and S100A9 siRNA-treated cells compared with controls. Thus, S100A8 and S100A9 promoted the migration of CNE1 NPC cells. PMID:26137102

R-Ras Regulates Migration through an Interaction with Filamin A in Melanoma Cells

PubMed Central

Gawecka, Joanna E.; Griffiths, Genevieve S.; Ek-Rylander, Barbro; Ramos, Joe W.; Matter, Michelle L.

2010-01-01

Background Changes in cell adhesion and migration in the tumor microenvironment are key in the initiation and progression of metastasis. R-Ras is one of several small GTPases that regulate cell adhesion and migration on the extracellular matrix, however the mechanism has not been completely elucidated. Using a yeast two-hybrid approach we sought to identify novel R-Ras binding proteins that might mediate its effects on integrins. Methods and Findings We identified Filamin A (FLNa) as a candidate interacting protein. FLNa is an actin-binding scaffold protein that also binds to integrin β1, β2 and β7 tails and is associated with diverse cell processes including cell migration. Indeed, M2 melanoma cells require FLNa for motility. We further show that R-Ras and FLNa interact in co-immunoprecipitations and pull-down assays. Deletion of FLNa repeat 3 (FLNaΔ3) abrogated this interaction. In M2 melanoma cells active R-Ras co-localized with FLNa but did not co-localize with FLNa lacking repeat 3. Thus, activated R-Ras binds repeat 3 of FLNa. The functional consequence of this interaction was that active R-Ras and FLNa coordinately increased cell migration. In contrast, co-expression of R-Ras and FLNaΔ3 had a significantly reduced effect on migration. While there was enhancement of integrin activation and fibronectin matrix assembly, cell adhesion was not altered. Finally, siRNA knockdown of endogenous R-Ras impaired FLNa-dependent fibronectin matrix assembly. Conclusions These data support a model in which R-Ras functionally associates with FLNa and thereby regulates integrin-dependent migration. Thus in melanoma cells R-Ras and FLNa may cooperatively promote metastasis by enhancing cell migration. PMID:20585650

TLR4 induces CREB-mediated IL-6 production via upregulation of F-spondin to promote vascular smooth muscle cell migration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Guan-Lin; Graduate Institutes of Life Sciences, National Defense Medical Center, Taipei, Taiwan; Wu, Jing-Yiing

Toll-like receptor 4 (TLR4) is important in promoting inflammation and vascular smooth muscle cell (VSMC) migration, both of which contribute to atherosclerosis development and progression. But the mechanism underlying the regulation of TLR4 in VSMC migration remains unclear. Stimulation of VSMCs with LPS increased the cellular level of F-spondin which is associated with the regulation of proinflammatory cytokine production. The LPS-induced F-spondin expression depended on TLR4-mediated PI3K/Akt pathway. Suppression of F-spondin level by siRNA inhibited not only F-spondin expression but also LPS-induced phosphorylation of cAMP response element binding protein (CREB) and IL-6 expression, VSMC migration and proliferation as well asmore » MMP9 expression. Moreover, suppression of CREB level by siRNA inhibited TLR4-induced IL-6 production and VSMC migration. Inhibition of F-spondin siRNA on LPS-induced migration was restored by addition of exogenous recombinant mouse IL-6. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. -- Highlights: •LPS-induced F-spondin expression of VSMCs is via a TLR4/PI3K/Akt signaling. •F-spondin is pivotal for LPS-induced CREB-mediated IL-6 production. •F-spondin is required for LPS-induced VSMC migration and proliferation.« less

Labour migration and rural transformation in post-colonial Swaziland.

PubMed

Simelane, H S

1995-07-01

This article describes the effects of labor migration in rural southern Swaziland. It is argued that wages from rural migration to mining areas were invested in agricultural implements such as tractors, in education of children, and in cattle. These effects are improvements in rural quality of life and involve women in agricultural production. Male labor migration in southern Swaziland contributed to progress and rural development for rich and poor families at the individual level. Community level changes from labor migration were not evident. The author does not suggest that all changes were due to labor migration or unique. Negative effects are recognized, such as life style changes or changes in social customs. It is stated that southern Swaziland benefited little post-independence from national development. The Lowveld benefitted from sugar and citrus plantations. The central region experienced development of industry and commerce. The northern region had sugar plantations and mining development. The western region established commercial forests. The south only had export labor, which was attracted to higher wages in South Africa. Data and arguments in this article pertain only to male labor migration to mining areas. The literature on development tends to associate male labor migration with underdevelopment. Women are left to plough the fields in traditional agricultural production. Evidence is presented for southern Swaziland, which showed periods of low agricultural production and poor health conditions. During the 1940s this was the case and male absence was very high. Although women had limitations, it is argued that the most important factor was unavailability of new technology. By the 1970s, women were able to hire private or government tractors for tilling the soil.

Walla Walla River Fish Passage Operations Program, 2003-2004 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bronson, James P.

2004-12-01

In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survivalmore » of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2003-2004 project year, there were 379 adult summer steelhead (Oncorhynchus mykiss), 36 adult bull trout (Salvelinus confluentus); 108 adult and 3 jack spring chinook (O. tshawytscha) enumerated at the Nursery Bridge Dam fishway video counting window between December 21, 2003, and June 30, 2004. Summer steelhead and spring chinook were observed moving upstream while bull trout were observed moving both upstream and downstream of the facility. In addition, the old ladder trap was operated by the WWBNPME project in order to radio tag spring chinook adults. A total of 2 adult summer steelhead, 4 bull trout, and 23 adult spring chinook were enumerated at the west ladder at Nursery Bridge Dam during the trapping operations between May 6 and May 23, 2004. Operation of the Little Walla Walla River juvenile trap for trap and haul purposes was not necessary this year. The project transported adult spring chinook from Threemile Dam to the South Fork Walla Walla Brood Holding Facility. A total of 239 spring chinook were outplanted in August for natural spawning in the basin.« less

Vertical migration of municipal wastewater in deep injection well systems, South Florida, USA

NASA Astrophysics Data System (ADS)

Maliva, Robert G.; Guo, Weixing; Missimer, Thomas

2007-11-01

Deep well injection is widely used in South Florida, USA for wastewater disposal largely because of the presence of an injection zone (“boulder zone” of Floridan Aquifer System) that is capable of accepting very large quantities of fluids, in some wells over 75,000 m3/day. The greatest potential risk to public health associated with deep injection wells in South Florida is vertical migration of wastewater, containing pathogenic microorganisms and pollutants, into brackish-water aquifer zones that are being used for alternative water-supply projects such as aquifer storage and recovery. Upwards migration of municipal wastewater has occurred in a minority of South Florida injection systems. The results of solute-transport modeling using the SEAWAT program indicate that the measured vertical hydraulic conductivities of the rock matrix would allow for only minimal vertical migration. Fracturing at some sites increased the equivalent average vertical hydraulic conductivity of confining zone strata by approximately four orders of magnitude and allowed for vertical migration rates of up 80 m/year. Even where vertical migration was rapid, the documented transit times are likely long enough for the inactivation of pathogenic microorganisms.

Increasing the realism of projected tree species ranges by incorporating migration potential: an eastern US case study

NASA Astrophysics Data System (ADS)

Rogers, B. M.; Jantz, P.; Goetz, S. J.

2015-12-01

Models of vegetation distributions are used for a wide variety of purposes, from global assessments of biome shifts and biogeochemical feedbacks to local management planning. Dynamic vegetation models, mostly mechanistic in origin, are valuable for regional to global studies but remain limited for more local-scale applications, especially those that require species-specific responses to climate change. Species distribution models (SDMs) are broadly used for such applications, but these too have several outstanding limitations, one of the most prominent being a lack of dispersal and migration. Several hybrid models have recently been developed, but these generally require detailed parameterization of species-level attributes that may not be known. Here we present an approach to couple migration potential with SDM output for a large number of species in order to more realistically project future range shifts. We focus on 40 tree species in the eastern US of potential management concern, either because of their canopy dominance, ecosystem functions, or potential for utilizing future climates. Future climates were taken from a CMIP5 model ensemble average using RCP 4.5 and 8.5 scenarios. We used Random Forests to characterize current and future environmental suitability, and modeled migration as a negative exponential kernel that is affected by forest fragmentation and the density of current seed sources. We present results in a vulnerability framework relevant for a number of ongoing management activities in the region. We find an overarching pattern of northward and eastward range shifts, with high-elevation and northern species being the most adversely impacted. Because of limitations to migration, many newly suitable areas could not be utilized without active intervention. Only a few areas exhibited consistently favorable conditions that could be utilized by the relevant species, including the central Appalachian foothills and the Florida panhandle. We suggest that a continued effort to include migration potential into vegetation models can lead to more realistic results and management-relevant products.

Seismic Reflectivity of the Crust in the Northern Salton Trough

NASA Astrophysics Data System (ADS)

Bauer, K.; Fuis, G. S.; Goldman, M.; Persaud, P.; Ryberg, T.; Langenheim, V. E.; Scheirer, D. S.; Rymer, M. J.; Hole, J. A.; Stock, J. M.; Catchings, R.

2015-12-01

The Salton Trough in southern California is a tectonically active pull-apart basin that was formed by migrating step-overs between strike-slip faults, of which the San Andreas Fault (SAF) and the Imperial Fault are the current, northernmost examples. The Salton Seismic Imaging Project (SSIP) was undertaken to improve our knowledge of fault geometry and seismic velocities within the sedimentary basins and underlying crystalline crust around the SAF. Such data are useful as input for modeling scenarios of strong ground shaking in the surrounding high-population areas. We used pre-stack depth migration of line segments from shot gathers in several seismic profiles that were acquired in the northern part of the SSIP study area (Lines 4 - 7). Our migration approach can be considered as an infinite-frequency approximation of the Fresnel volume pre-stack depth migration method. We use line segments instead of the original waveform data. We demonstrate the method using synthetic data and analyze real data from Lines 4 - 7 to illustrate the relationship between distinct phases in the time domain and their resulting image at depth. We show both normal-moveout reflections from sub-horizontal interfaces and reverse-moveout reflections from steep interfaces, such as faults. Migrated images of dipping faults, such as the SAF and the Pinto Mountain Fault, are presented in this way. The SAF is imaged along Line 4, through the Mecca Hills, as a number of steeply dipping fault segments that collectively form a flower structure, above 5 km depth, that sole into a moderately NE-dipping fault below that depth. The individual migrated reflection packages correlate with mapped surface fault traces in the Mecca Hills. A similar geometry is seen on Line 6, from Palm Springs through Yucca Valley, where fault splays sole or project into a moderately dipping SAF below 10-km depth. We also show and discuss the reflectivity pattern of the middle and lower crust for Lines 4 - 7.

Inhibition of AQP1 Hampers Osteosarcoma and Hepatocellular Carcinoma Progression Mediated by Bone Marrow-Derived Mesenchymal Stem Cells.

PubMed

Pelagalli, Alessandra; Nardelli, Anna; Fontanella, Raffaela; Zannetti, Antonella

2016-07-11

The complex cross-talk between tumor cells and their surrounding stromal environment plays a key role in the pathogenesis of cancer. Among several cell types that constitute the tumor stroma, bone marrow-derived mesenchymal stem cells (BM-MSCs) selectively migrate toward the tumor microenvironment and contribute to the active formation of tumor-associated stroma. Therefore, here we elucidate the involvement of BM-MSCs to promote osteosarcoma (OS) and hepatocellular carcinoma (HCC) cells migration and invasion and deepening the role of specific pathways. We analyzed the function of aquaporin 1 (AQP1), a water channel known to promote metastasis and neoangiogenes. AQP1 protein levels were analyzed in OS (U2OS) and HCC (SNU-398) cells exposed to conditioned medium from BM-MSCs. Tumor cell migration and invasion in response to BM-MSC conditioned medium were evaluated through a wound healing assay and Boyden chamber, respectively. The results showed that the AQP1 level was increased in both tumor cell lines after treatment with BM-MSC conditioned medium. Moreover, BM-MSCs-mediated tumor cell migration and invasion were hampered after treatment with AQP1 inhibitor. These data suggest that the recruitment of human BM-MSCs into the tumor microenvironment might cause OS and HCC cell migration and invasion through involvement of AQP1.

MicroRNA-146a promote cell migration and invasion in human colorectal cancer via carboxypeptidase M/src-FAK pathway

PubMed Central

Zhan, Cheng; Le-Meng, Zhang; Liu, Hongchun; Cai, Yu; Tu, Chuantao; Li, Xi; Zou, Yanting; Zhang, Shuncai

2017-01-01

Colorectal cancer (CRC) is one of the most common cancers worldwide, and microRNAs play important roles in CRC progression. This study aimed to investigate the roles of miR-146a-5p in human CRC and their molecular mechanisms. First, we found that miR-146a-5p was significantly upregulated in CRC tissues and promoted the migration of CRC cells. Then, we identified carboxypeptidase M (CPM) as a direct target of miR-146a-5p, and found that it inhibited the migration and invasion of CRC cells. Our results also showed that CPM expression was positively correlated with overall survival and negatively correlated with recurrence, lymph node invasion, and N stage. Furthermore, we demonstrated that both miR-146a-5p and CPM regulated Src and FAK expression, while the Src-FAK signaling pathway is widely known to be associated with the migration and invasion of multiple tumor cells. This study is the first to demonstrate the functional and mechanistic relationship of the miR-146a-5p/CPM/Src-FAK axis and its effect on the migration and invasion of CRC cells. Thus, miR-146a-5p represents potential targets for CRC diagnosis and therapy. PMID:28186967

Recent and rapid population growth and range expansion of the Lyme disease tick vector, Ixodes scapularis, in North America.

PubMed

Khatchikian, Camilo E; Prusinski, Melissa A; Stone, Melissa; Backenson, Peter Bryon; Wang, Ing-Nang; Foley, Erica; Seifert, Stephanie N; Levy, Michael Z; Brisson, Dustin

2015-07-01

Migration is a primary force of biological evolution that alters allele frequencies and introduces novel genetic variants into populations. Recent migration has been proposed as the cause of the emergence of many infectious diseases, including those carried by blacklegged ticks in North America. Populations of blacklegged ticks have established and flourished in areas of North America previously thought to be devoid of this species. The recent discovery of these populations of blacklegged ticks may have resulted from either in situ growth of long-established populations that were maintained at very low densities or by migration and colonization from established populations. These alternative evolutionary hypotheses were investigated using Bayesian phylogeographic approaches to infer the origin and migratory history of recently detected blacklegged tick populations in the Northeastern United States. The data and results indicate that newly detected tick populations are not the product of in situ population growth from a previously established population but from recent colonization resulting in a geographic range expansion. This expansion in the geographic range proceeded primarily through progressive and local migration events from southern populations to proximate northern locations although long-distance migration events were also detected. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - β1 (TGF-β1) and potential effects on migration and invasion.

PubMed

Magnussen, Synnove Norvoll; Hadler-Olsen, Elin; Costea, Daniela Elena; Berg, Eli; Jacobsen, Cristiane Cavalcanti; Mortensen, Bente; Salo, Tuula; Martinez-Zubiaurre, Inigo; Winberg, Jan-Olof; Uhlin-Hansen, Lars; Svineng, Gunbjorg

2017-05-19

Urokinase plasminogen activator (uPA) receptor (uPAR) is up-regulated at the invasive tumour front of human oral squamous cell carcinoma (OSCC), indicating a role for uPAR in tumour progression. We previously observed elevated expression of uPAR at the tumour-stroma interface in a mouse model for OSCC, which was associated with increased proteolytic activity. The tumour microenvironment regulated uPAR expression, as well as its glycosylation and cleavage. Both full-length- and cleaved uPAR (uPAR (II-III)) are involved in highly regulated processes such as cell signalling, proliferation, migration, stem cell mobilization and invasion. The aim of the current study was to analyse tumour associated factors and their effect on uPAR cleavage, and the potential implications for cell proliferation, migration and invasion. Mouse uPAR was stably overexpressed in the mouse OSCC cell line AT84. The ratio of full-length versus cleaved uPAR as analysed by Western blotting and its regulation was assessed by addition of different protease inhibitors and transforming growth factor - β1 (TGF-β1). The role of uPAR cleavage in cell proliferation and migration was analysed using real-time cell analysis and invasion was assessed using the myoma invasion model. We found that when uPAR was overexpressed a proportion of the receptor was cleaved, thus the cells presented both full-length uPAR and uPAR (II-III). Cleavage was mainly performed by serine proteases and urokinase plasminogen activator (uPA) in particular. When the OSCC cells were stimulated with TGF-β1, the production of the uPA inhibitor PAI-1 was increased, resulting in a reduction of uPAR cleavage. By inhibiting cleavage of uPAR, cell migration was reduced, and by inhibiting uPA activity, invasion was reduced. We could also show that medium containing soluble uPAR (suPAR), and cleaved soluble uPAR (suPAR (II-III)), induced migration in OSCC cells with low endogenous levels of uPAR. These results show that soluble factors in the tumour microenvironment, such as TGF-β1, PAI-1 and uPA, can influence the ratio of full length and uPAR (II-III) and thereby potentially effect cell migration and invasion. Resolving how uPAR cleavage is controlled is therefore vital for understanding how OSCC progresses and potentially provides new targets for therapy.

Annual DOE active solar heating and cooling contractors' review meeting. Premeeting proceedings and project summaries

DOE Office of Scientific and Technical Information (OSTI.GOV)

None,

1981-09-01

Ninety-three project summaries are presented which discuss the following aspects of active solar heating and cooling: Rankine solar cooling systems; absorption solar cooling systems; desiccant solar cooling systems; solar heat pump systems; solar hot water systems; special projects (such as the National Solar Data Network, hybrid solar thermal/photovoltaic applications, and heat transfer and water migration in soils); administrative/management support; and solar collector, storage, controls, analysis, and materials technology. (LEW)

Effect of perineoplasm perinephric adipose tissues on migration of clear cell renal cell carcinoma cells: a potential role of WNT signaling.

PubMed

Zi, Xiaolin; Lusch, Achim; Blair, Christopher A; Okhunov, Zhamshid; Yokoyama, Noriko N; Liu, Shuman; Baker, Molly; Huynh, Victor; Landman, Jaime

2016-08-16

To investigate the cellular and molecular interactions between clear-cell renal cell carcinoma (ccRCC) and perinephric adipose tissue (PAT), perineoplasm PAT, PAT away from the neoplasm, renal sinus and subcutaneous adipose tissues were collected at the time of renal surgery for renal masses and conditioned medium (CM) was generated from 62 patients. Perineoplasm PAT CMs from 44 out of 62 (about 71%) of patients with ccRCC or benign renal diseases (e.g. oncocytomas, angiomyolipomas, multicystic kidney, interstitial fibrosis, etc.) enhanced the migration of CaKi-2 cells. Perineoplasm PAT CMs from ccRCC significantly increased migration of ACHN and CaKi-2 cells by ~8.2 and ~2.4 folds, respectively, relative to those from benign renal diseases, whereas there is no significant difference in migration between ccRCC and benign renal diseases in CMs collected from culturing PAT away from neoplasm, renal sinus and subcutaneous adipose tissues. High Fuhrman Grade was associated with increased migration of Caki-2 cells by perineoplasm PAT CMs. Perineoplasm PATs from pT3 RCCs overexpressed multiple WNTs and their CMs exhibited higher WNT/ß-catenin activity and increased the migration of Caki-2 cells compared to CMs from benign neoplasms. Addition of secreted WNT inhibitory factor-1 recombinant protein into perineoplasm PAT CMs completely blocked the cell migration. These results indicate that WNT related factors from perineoplasm PAT may promote progression of local ccRCC to locally advanced (pT3) disease by increasing ccRCC cell mobility.

Caribbean nurses migrating to the UK: a gender-focused literature review.

PubMed

Jones, A D; Bifulco, A; Gabe, J

2009-09-01

International nurse recruitment is an integral part of government health care strategy in many countries. However, the gendered implications of nurse migration have been little explored despite the fact that the nursing workforce is predominantly made up of women. Based on the migration of nurses from the English-speaking Caribbean region to the UK, this paper explores the significance of gender at both the macro and micro levels. Four strands of inquiry were explored: nurse migration, impact on development, work experiences and family life. Key terms were used to search the electronic databases SSCI, EBSCO and JSTOR. An interpretative framework based on the feminist theory of intersectionality was used to systematically review the 15 studies that met the inclusion criteria. Gender issues are significant across all aspects of the migratory process. Migrant nurses contribute to social progress through remittances and knowledge gained abroad although overall, nurse migration negatively impacts development and there are hidden implications for women. For some Caribbean nurses, migration reflects increased economic freedom; however, for others, gender inequality lies at the centre of the decision to relocate. Gender inequality also permeates the lives of many migrant nurses even in countries where economic and work conditions are improved. The ramifications of nurse migration cannot be fully understood without attention to gender inequalities and the specific socio-economic contexts in which they exist. There is need for a gender-centred approach to international nursing recruitment policy that takes account not only of the impact on developing countries, but also of the well-being of migrant nurses themselves.

MicroRNA-9 regulates non-small cell lung cancer cell invasion and migration by targeting eukaryotic translation initiation factor 5A2.

PubMed

Xu, Guodong; Shao, Guofeng; Pan, Qiaoling; Sun, Lebo; Zheng, Dawei; Li, Minghui; Li, Ni; Shi, Huoshun; Ni, Yiming

2017-01-01

MicroRNAs (miRNAs) play a critical role in cancer development and progression. Bioinformatics analyses has identified eukaryotic translation initiation factor 5A2 (eIF5A2) as a target of miR-9. In this study, we attempted to determine whether miR-9 regulates non-small cell lung cancer (NSCLC) cell invasion and migration by targeting eIF5A2 We examined eIF5A2 expression using reverse transcription-quantitative PCR (RT-qPCR) and subsequently transfected A549 and NCI-H1299 NSCLC cells with a miR-9 mimic or miR-9 inhibitor to determine the migration and invasive capability of the cells via wound healing assay and Transwell invasion assay, respectively. E-cadherin and vimentin expression was detected with western blotting. The miR-9 mimic significantly reduced NSCLC cell invasive and metastatic ability, and the miR-9 inhibitor enhanced NSCLC cell migration activity, increasing the number of migrated cells. There was no significant difference between the negative control siRNA and miR-9 mimic groups after knockdown of eIF5A2; western blotting showed that miR-9 regulated E-cadherin and vimentin expression. These data show that miR-9 regulates NSCLC cell invasion and migration through regulating eIF5A2 expression. Taken together, our findings suggest that the mechanism of miR-9-regulated NSCLC cell invasion and migration may be related to epithelial-mesenchymal transition.

Radionuclide Migation Project 1984 progress report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buddemeier, R.W.; Isherwood, D.

1985-04-01

The report discusses the hydrogeologic settings and histories of studies associated with the Cheshire (U20n), Cambric (U5e), Nash (UE2ce), Bilby (U3cn), Bourbon (U7n), and Faultless (UC1) Events. Radionuclide and some chemical data are presented for water samples from cavity or chimney wells associated with the Cheshire, Cambric, and Bilby Events, and from satellite wells at the Cambric, Nash, Bibly, Bourbon, and Faultless Event sites. The report also gives the results of studies of specific sampling or analytical methodologies. These studies demonstrated that the apparent migration of /sup 155/Eu is an artfact of spectrometric misidentification of gamma- and x-ray peaks frommore » other constituents. A potential problem with atmospheric contamination of samples collected with evacuated thief samples was also identified. Ultrafiltration techniques were applied to some of the Cheshire cavity samples collected, and preliminary results suggest that substantial amounts of activity may be associated with colloidal particles in the size range of 0.006 to 0.45 ..mu..m. A study has begun of the recharge of effluent water from RNM-2S (Cambric satellite well) into the desert floor as a result of nine years of continuous pumping. This report gives the initial results of unsaturated zone studies showing the propagation of moisture and tritium fronts through the shallow soil. Geochemical modeling of the behavior of ruthenium and technetium was carried out, with particular emphasis on the identification of ionic species that would be potentially mobile under NTS ground-water conditions. The report compares the results with observations of ruthenium migration to the Cambric satellite well.« less

FY2017 Electrification Annual Progress Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

During fiscal year 2017 (FY 2017), the U.S. Department of Energy (DOE) Vehicle Technologies Office (VTO) funded early stage research & development (R&D) projects that address Batteries and Electrification of the U.S. transportation sector. The VTO Electrification Sub-Program is composed of Electric Drive Technologies, and Grid Integration activities. The Electric Drive Technologies group conducts R&D projects that advance Electric Motors and Power Electronics technologies. The Grid and Charging Infrastructure group conducts R&D projects that advance Grid Modernization and Electric Vehicle Charging technologies. This document presents a brief overview of the Electrification Sub-Program and progress reports for its R&D projects. Eachmore » of the progress reports provide a project overview and highlights of the technical results that were accomplished in FY 2017.« less

FLORIDA MIGRANT HEALTH PROJECT. FOURTH ANNUAL PROGRESS REPORT, 1966-1967.

ERIC Educational Resources Information Center

Florida State Board of Health, Jacksonville.

THE FOURTH ANNUAL PROGRESS REPORT OF THE FLORIDA MIGRANT HEALTH PROJECT INDICATES THAT IN 1966-67 THERE WAS AN APPRECIABLE INCREASE IN THE AMOUNT AND VARIETY OF MIGRANT HEALTH SERVICES RENDERED, THE NUMBER OF MIGRANTS CONTACTED, AND THE ACTIVITIES PERFORMED BY PROJECT PERSONNEL. MIGRANT HEALTH SERVICE REFERRALS INCREASED BY 1,222 OVER THE SAME…

Solar Thermal Power Systems parabolic dish project

NASA Technical Reports Server (NTRS)

Truscello, V. C.

1981-01-01

The status of the Solar Thermal Power Systems Project for FY 1980 is summarized. Included is: a discussion of the project's goals, program structure, and progress in parabolic dish technology. Analyses and test results of concentrators, receivers, and power converters are discussed. Progress toward the objectives of technology feasibility, technology readiness, system feasibility, and system readiness are covered.

105. View of tracking radome replacement project, progress photograph, official ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

105. View of tracking radome replacement project, progress photograph, official photograph BMEWS Project by unknown photographer, 11 August 1981, clear as negative no. A-18562. - Clear Air Force Station, Ballistic Missile Early Warning System Site II, One mile west of mile marker 293.5 on Parks Highway, 5 miles southwest of Anderson, Anderson, Denali Borough, AK

Observing Migration and Burial of Unexploded Ordnance in the Nearshore Environment with Instrumented Surrogates

NASA Astrophysics Data System (ADS)

Bruder, B. L.; Cristaudo, D.; Puleo, J. A.

2016-12-01

Prior to 1972, it was legal and common practice to unload unexploded ordnance (UXO) into the ocean. Only 60-100 miles off the US coast alone there are 72 dumping sites where it is estimated 31 million pounds of UXO lie. As recently as 2015, UXO have been found not only in the nearshore environment, but on populated beaches. Thus, understanding the migration and burial of these objects is not only of oceanographic interest, but a matter of public safety. The presented project evaluates the efficacy of instrumented UXO surrogates for observing munition migration and burial. Instrumented surrogates were exposed to near prototype scale wave conditions over a mobile bed at the Littoral Warfare Environment at Aberdeen Test Center, MD. Surrogates were deployed in the swash zone, inner and outer surf zones. Dependent on munition size, surrogates housed multiple suites of self-logging sensors. Sensor suites included different combinations of inertial motion units, ultra-wideband tracking tags, pressure transducers, shock recorders, and photocells. Preliminary results show sensor suites can resolve various types of surrogate movement. Pressure transducers accurately record ambient wave conditions as well as changes in mean depth due to surrogate migration. Inertial motion units resolve munition accelerations for rolling and translational motion. Inertial motion unit data is used to estimate trajectory as well when coupled with mean depth and bathymetric data. Photocells, which measure ambient light, resolve munition burial as well as serve as proxies for surrounding environmental conditions such as suspended sediment and water depth. The presented project will continue to utilize and couple surrogate sensor data to resolve munition movement and burial under different conditions. Knowledge of munition migration helps focus UXO detection and recovery, conserving US military and coastal resources.

Making Progress: The Use of Multiple Progress Reports to Enhance Advertising Students' Media Plan Term Projects

ERIC Educational Resources Information Center

Kritz, Gary H.; Lozada, Hector R.; Long, Mary M.

2007-01-01

Since the AACSB mandates that students demonstrate effective oral and written communication skills, it is imperative that business professors do what is necessary to improve such skills. The authors investigate whether the use of using multiple progress reports in an Advertising class project improves the final product. The data results show that…

Hydrologic Resources Management Program and Underground Tests Area Project FY 2003 Progress Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

J., B C; F., E G; K., E B

This report describes FY 2003 technical studies conducted by the Chemical Biology and Nuclear Science Division (CBND) at Lawrence Livermore National Laboratory (LLNL) in support of the Hydrologic Resources Management Program (HRMP) and the Underground Test Area (UGTA) Project. These programs are administered by the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) through the Defense Programs and Environmental Restoration Divisions, respectively. HRMP-sponsored work is directed toward the responsible management of the natural resources at the Nevada Test Site (NTS), enabling its continued use as a staging area for strategic operations in support of national security.more » UGTA-funded work emphasizes the development of an integrated set of groundwater flow and contaminant transport models to predict the extent of radionuclide migration from underground nuclear testing areas at the NTS. The present report is organized on a topical basis and contains five chapters that reflect the range of technical work performed by LLNL-CBND during FY 2003. Although we have emphasized investigations that were led by CBND, we also participated in a variety of collaborative studies with other UGTA and HRMP contract organizations including the Energy and Environment Directorate at LLNL (LLNL-E&E), Los Alamos National Laboratory (LANL), the Desert Research Institute (DRI), the U.S. Geological Survey (USGS), Stoller-Navarro Joint Venture (SNJV), and Bechtel Nevada (BN).« less

Applying remote sensing expertise to crop improvement: progress and challenges to scale up high throughput field phenotyping from research to industry

NASA Astrophysics Data System (ADS)

Gouache, David; Beauchêne, Katia; Mini, Agathe; Fournier, Antoine; de Solan, Benoit; Baret, Fred; Comar, Alexis

2016-05-01

Digital and image analysis technologies in greenhouses have become commonplace in plant science research and started to move into the plant breeding industry. However, the core of plant breeding work takes place in fields. We will present successive technological developments that have allowed the migration and application of remote sensing approaches at large into the field of crop genetics and physiology research, with a number of projects that have taken place in France. These projects have allowed us to develop combined sensor plus vector systems, from tractor mounted and UAV (unmanned aerial vehicle) mounted spectroradiometry to autonomous vehicle mounted spectroradiometry, RGB (red-green-blue) imagery and Lidar. We have tested these systems for deciphering the genetics of complex plant improvement targets such as the robustness to nitrogen and water deficiency of wheat and maize. Our results from wheat experiments indicate that these systems can be used both to screen genetic diversity for nitrogen stress tolerance and to decipher the genetics behind this diversity. We will present our view on the next critical steps in terms of technology and data analysis that will be required to reach cost effective implementation in industrial plant breeding programs. If this can be achieved, these technologies will largely contribute to resolving the equation of increasing food supply in the resource limited world that lies ahead.

Demographic models and IPCC climate projections predict the decline of an emperor penguin population.

PubMed

Jenouvrier, Stéphanie; Caswell, Hal; Barbraud, Christophe; Holland, Marika; Stroeve, Julienne; Weimerskirch, Henri

2009-02-10

Studies have reported important effects of recent climate change on Antarctic species, but there has been to our knowledge no attempt to explicitly link those results to forecasted population responses to climate change. Antarctic sea ice extent (SIE) is projected to shrink as concentrations of atmospheric greenhouse gases (GHGs) increase, and emperor penguins (Aptenodytes forsteri) are extremely sensitive to these changes because they use sea ice as a breeding, foraging and molting habitat. We project emperor penguin population responses to future sea ice changes, using a stochastic population model that combines a unique long-term demographic dataset (1962-2005) from a colony in Terre Adélie, Antarctica and projections of SIE from General Circulation Models (GCM) of Earth's climate included in the most recent Intergovernmental Panel on Climate Change (IPCC) assessment report. We show that the increased frequency of warm events associated with projected decreases in SIE will reduce the population viability. The probability of quasi-extinction (a decline of 95% or more) is at least 36% by 2100. The median population size is projected to decline from approximately 6,000 to approximately 400 breeding pairs over this period. To avoid extinction, emperor penguins will have to adapt, migrate or change the timing of their growth stages. However, given the future projected increases in GHGs and its effect on Antarctic climate, evolution or migration seem unlikely for such long lived species at the remote southern end of the Earth.

Demographic models and IPCC climate projections predict the decline of an emperor penguin population

PubMed Central

Jenouvrier, Stéphanie; Caswell, Hal; Barbraud, Christophe; Holland, Marika; Strœve, Julienne; Weimerskirch, Henri

2009-01-01

Studies have reported important effects of recent climate change on Antarctic species, but there has been to our knowledge no attempt to explicitly link those results to forecasted population responses to climate change. Antarctic sea ice extent (SIE) is projected to shrink as concentrations of atmospheric greenhouse gases (GHGs) increase, and emperor penguins (Aptenodytes forsteri) are extremely sensitive to these changes because they use sea ice as a breeding, foraging and molting habitat. We project emperor penguin population responses to future sea ice changes, using a stochastic population model that combines a unique long-term demographic dataset (1962–2005) from a colony in Terre Adélie, Antarctica and projections of SIE from General Circulation Models (GCM) of Earth's climate included in the most recent Intergovernmental Panel on Climate Change (IPCC) assessment report. We show that the increased frequency of warm events associated with projected decreases in SIE will reduce the population viability. The probability of quasi-extinction (a decline of 95% or more) is at least 36% by 2100. The median population size is projected to decline from ≈6,000 to ≈400 breeding pairs over this period. To avoid extinction, emperor penguins will have to adapt, migrate or change the timing of their growth stages. However, given the future projected increases in GHGs and its effect on Antarctic climate, evolution or migration seem unlikely for such long lived species at the remote southern end of the Earth. PMID:19171908

New Jersey Land-Use Planning Techniques and Legislation. Agricultural Experiment Station Bulletin AE-338.

ERIC Educational Resources Information Center

Schneider, Lee D.

In response to recent urban to rural migration trends and the development of rather piecemeal land use policies and practices by local, state, and Federal decision makers, the U.S. Department of Agriculture has established a regional project (NE-78) and this report reflects the first of three major project objectives (to describe and appraise…

Simulating the Effects of Sea Level Rise on the Resilience and Migration of Tidal Wetlands along the Hudson River

PubMed Central

Tabak, Nava M.; Laba, Magdeline; Spector, Sacha

2016-01-01

Sea Level Rise (SLR) caused by climate change is impacting coastal wetlands around the globe. Due to their distinctive biophysical characteristics and unique plant communities, freshwater tidal wetlands are expected to exhibit a different response to SLR as compared with the better studied salt marshes. In this study we employed the Sea Level Affecting Marshes Model (SLAMM), which simulates regional- or local-scale changes in tidal wetland habitats in response to SLR, and adapted it for application in a freshwater-dominated tidal river system, the Hudson River Estuary. Using regionally-specific estimated ranges of SLR and accretion rates, we produced simulations for a spectrum of possible future wetland distributions and quantified the projected wetland resilience, migration or loss in the HRE through the end of the 21st century. Projections of total wetland extent and migration were more strongly determined by the rate of SLR than the rate of accretion. Surprisingly, an increase in net tidal wetland area was projected under all scenarios, with newly-formed tidal wetlands expected to comprise at least 33% of the HRE’s wetland area by year 2100. Model simulations with high rates of SLR and/or low rates of accretion resulted in broad shifts in wetland composition with widespread conversion of high marsh habitat to low marsh, tidal flat or permanent inundation. Wetland expansion and resilience were not equally distributed through the estuary, with just three of 48 primary wetland areas encompassing >50% of projected new wetland by the year 2100. Our results open an avenue for improving predictive models of the response of freshwater tidal wetlands to sea level rise, and broadly inform the planning of conservation measures of this critical resource in the Hudson River Estuary. PMID:27043136

Simulating the Effects of Sea Level Rise on the Resilience and Migration of Tidal Wetlands along the Hudson River.

PubMed

Tabak, Nava M; Laba, Magdeline; Spector, Sacha

2016-01-01

Sea Level Rise (SLR) caused by climate change is impacting coastal wetlands around the globe. Due to their distinctive biophysical characteristics and unique plant communities, freshwater tidal wetlands are expected to exhibit a different response to SLR as compared with the better studied salt marshes. In this study we employed the Sea Level Affecting Marshes Model (SLAMM), which simulates regional- or local-scale changes in tidal wetland habitats in response to SLR, and adapted it for application in a freshwater-dominated tidal river system, the Hudson River Estuary. Using regionally-specific estimated ranges of SLR and accretion rates, we produced simulations for a spectrum of possible future wetland distributions and quantified the projected wetland resilience, migration or loss in the HRE through the end of the 21st century. Projections of total wetland extent and migration were more strongly determined by the rate of SLR than the rate of accretion. Surprisingly, an increase in net tidal wetland area was projected under all scenarios, with newly-formed tidal wetlands expected to comprise at least 33% of the HRE's wetland area by year 2100. Model simulations with high rates of SLR and/or low rates of accretion resulted in broad shifts in wetland composition with widespread conversion of high marsh habitat to low marsh, tidal flat or permanent inundation. Wetland expansion and resilience were not equally distributed through the estuary, with just three of 48 primary wetland areas encompassing >50% of projected new wetland by the year 2100. Our results open an avenue for improving predictive models of the response of freshwater tidal wetlands to sea level rise, and broadly inform the planning of conservation measures of this critical resource in the Hudson River Estuary.

Healthier before they migrate, less healthy when they return? The health of returned migrants in Mexico

PubMed Central

Ullmann, S. Heidi; Goldman, Noreen; Massey, Douglas S.

2011-01-01

Over the course of the 20th century, Mexico-U.S. migration has emerged as an important facet of both countries, with far reaching economic and social impacts. The health of Mexican immigrants in the U.S. has been well studied, but relatively less is known about the health of returned migrants to Mexico. The objectives of this paper are twofold. Relying on health data pertaining to two stages of the life course, early life health (pre-migration) and adult health (post-migration) from the Mexican Migration Project gathered between 2007 and 2009, we aim to assess disparities in adult health status between male returned migrants and male non-migrants in Mexico, accounting for their potentially different early life health profiles. While we find evidence that returned migrants had more favorable early life health, the results for adult health are more complex. Returned migrants have a higher prevalence of heart disease, emotional/psychiatric disorders, obesity, and smoking than non-migrants but no differences are found in self-rated health, diabetes, or hypertension. PMID:21729820

Health, migration and border management: analysis and capacity-building at Europe's borders.

PubMed

Hollings, Jennifer; Samuilova, Mariya; Petrova-Benedict, Roumyana

2012-04-01

Three key elements were analysed in Hungary, Poland and Slovakia as a basis for strengthening the capacity of staff and structures related to health, migration and border management: public health concerns linked to migration, health needs and rights of migrants and the occupational health of staff. This IOM project was implemented through an in-depth situation analysis as well as the development of training modules and public health guidelines. Findings indicate a paucity of existing data, gaps in the health care for migrants and few existing tools for border officials and health professionals. Sets of training modules were developed for each of these groups, including common modules on migration and the right to health and intercultural communication, as well as targeted health modules. The guidelines promote good practices in the context of border management and detention. The EU is working towards a common immigration policy and integrated border management; however, a harmonized approach to migration and health is still lacking. Further research and piloting of the developed materials is needed to fully establish an adaptable, common toolkit.

INTERNATIONAL MIGRATION AND THE EDUCATION OF CHILDREN: EVIDENCE FROM LIMA, PERU

PubMed Central

Robles, Verónica Frisancho; Oropesa, R.S.

2012-01-01

The issue of whether emigration has consequences for the education of children who remain behind in the country of origin occupies an increasingly prominent place in the agendas of both scholars and policy makers. The conventional wisdom is that the emigration of family members may benefit children by relaxing budget constraints through remittances that can be used to cover educational expenses. However, the empirical evidence on the overall effect of migration is inconclusive. This is due in part to a substantive emphasis on remittances in the literature, as well as the inability of some studies to deal satisfactorily with the endogeneity of household migration decisions in comparing outcomes across migrant and non-migrant households. Using Peruvian data from the Latin American Migration Project (LAMP), we apply an innovative instrumental variable technique to evaluate the overall effect of migration on educational attainment and schooling disruption among the children of immigrants. In contrast to conventional wisdom, our results suggest that a higher household risk of immigration has deleterious consequences for the education of children who remain behind. PMID:22581990

Inhibition or ablation of transglutaminase 2 impairs astrocyte migration.

PubMed

Monteagudo, Alina; Ji, Changyi; Akbar, Abdullah; Keillor, Jeffrey W; Johnson, Gail V W

2017-01-22

Astrocytes play numerous complex roles that support and facilitate the function of neurons. Further, when there is an injury to the central nervous system (CNS) they can both facilitate or ameliorate functional recovery depending on the location and severity of the injury. When a CNS injury is relatively severe a glial scar is formed, which is primarily composed of astrocytes. The glial scar can be both beneficial, by limiting inflammation, and detrimental, by preventing neuronal projections, to functional recovery. Thus, understanding the processes and proteins that regulate astrocyte migration in response to injury is still of fundamental importance. One protein that is likely involved in astrocyte migration is transglutaminase 2 (TG2); a multifunctional protein expressed ubiquitously throughout the brain. Its functions include transamidation and GTPase activity, among others, and previous studies have implicated TG2 as a regulator of migration. Therefore, we examined the role of TG2 in primary astrocyte migration subsequent to injury. Using wild type or TG2 -/- astrocytes, we manipulated the different functions and conformation of TG2 with novel irreversible inhibitors or mutant versions of the protein. Results showed that both inhibition and ablation of TG2 in primary astrocytes significantly inhibit migration. Additionally, we show that the deficiency in migration caused by deletion of TG2 can only be rescued with the native protein and not with mutants. Finally, the addition of TGFβ rescued the migration deficiency independent of TG2. Taken together, our study shows that transamidation and GTP/GDP-binding are necessary for inhibiting astrocyte migration and it is TGFβ independent. Copyright © 2016 Elsevier Inc. All rights reserved.

Hox paralog group 2 genes control the migration of mouse pontine neurons through slit-robo signaling.

PubMed

Geisen, Marc J; Di Meglio, Thomas; Pasqualetti, Massimo; Ducret, Sebastien; Brunet, Jean-François; Chedotal, Alain; Rijli, Filippo M

2008-06-10

The pontine neurons (PN) represent a major source of mossy fiber projections to the cerebellum. During mouse hindbrain development, PN migrate tangentially and sequentially along both the anteroposterior (AP) and dorsoventral (DV) axes. Unlike DV migration, which is controlled by the Netrin-1/Dcc attractive pathway, little is known about the molecular mechanisms guiding PN migration along the AP axis. Here, we show that Hoxa2 and Hoxb2 are required both intrinsically and extrinsically to maintain normal AP migration of subsets of PN, by preventing their premature ventral attraction towards the midline. Moreover, the migration defects observed in Hoxa2 and Hoxb2 mutant mice were phenocopied in compound Robo1;Robo2, Slit1;Slit2, and Robo2;Slit2 knockout animals, indicating that these guidance molecules act downstream of Hox genes to control PN migration. Indeed, using chromatin immunoprecipitation assays, we further demonstrated that Robo2 is a direct target of Hoxa2 in vivo and that maintenance of high Robo and Slit expression levels was impaired in Hoxa2 mutant mice. Lastly, the analysis of Phox2b-deficient mice indicated that the facial motor nucleus is a major Slit signaling source required to prevent premature ventral migration of PN. These findings provide novel insights into the molecular control of neuronal migration from transcription factor to regulation of guidance receptor and ligand expression. Specifically, they address the question of how exposure to multiple guidance cues along the AP and DV axes is regulated at the transcriptional level and in turn translated into stereotyped migratory responses during tangential migration of neurons in the developing mammalian brain.

Hox Paralog Group 2 Genes Control the Migration of Mouse Pontine Neurons through Slit-Robo Signaling

PubMed Central

Pasqualetti, Massimo; Ducret, Sebastien; Brunet, Jean-François; Chedotal, Alain; Rijli, Filippo M

2008-01-01

The pontine neurons (PN) represent a major source of mossy fiber projections to the cerebellum. During mouse hindbrain development, PN migrate tangentially and sequentially along both the anteroposterior (AP) and dorsoventral (DV) axes. Unlike DV migration, which is controlled by the Netrin-1/Dcc attractive pathway, little is known about the molecular mechanisms guiding PN migration along the AP axis. Here, we show that Hoxa2 and Hoxb2 are required both intrinsically and extrinsically to maintain normal AP migration of subsets of PN, by preventing their premature ventral attraction towards the midline. Moreover, the migration defects observed in Hoxa2 and Hoxb2 mutant mice were phenocopied in compound Robo1;Robo2, Slit1;Slit2, and Robo2;Slit2 knockout animals, indicating that these guidance molecules act downstream of Hox genes to control PN migration. Indeed, using chromatin immunoprecipitation assays, we further demonstrated that Robo2 is a direct target of Hoxa2 in vivo and that maintenance of high Robo and Slit expression levels was impaired in Hoxa2 mutant mice. Lastly, the analysis of Phox2b-deficient mice indicated that the facial motor nucleus is a major Slit signaling source required to prevent premature ventral migration of PN. These findings provide novel insights into the molecular control of neuronal migration from transcription factor to regulation of guidance receptor and ligand expression. Specifically, they address the question of how exposure to multiple guidance cues along the AP and DV axes is regulated at the transcriptional level and in turn translated into stereotyped migratory responses during tangential migration of neurons in the developing mammalian brain. PMID:18547144

Quantitative cell polarity imaging defines leader-to-follower transitions during collective migration and the key role of microtubule-dependent adherens junction formation.

PubMed

Revenu, Céline; Streichan, Sebastian; Donà, Erika; Lecaudey, Virginie; Hufnagel, Lars; Gilmour, Darren

2014-03-01

The directed migration of cell collectives drives the formation of complex organ systems. A characteristic feature of many migrating collectives is a 'tissue-scale' polarity, whereby 'leader' cells at the edge of the tissue guide trailing 'followers' that become assembled into polarised epithelial tissues en route. Here, we combine quantitative imaging and perturbation approaches to investigate epithelial cell state transitions during collective migration and organogenesis, using the zebrafish lateral line primordium as an in vivo model. A readout of three-dimensional cell polarity, based on centrosomal-nucleus axes, allows the transition from migrating leaders to assembled followers to be quantitatively resolved for the first time in vivo. Using live reporters and a novel fluorescent protein timer approach, we investigate changes in cell-cell adhesion underlying this transition by monitoring cadherin receptor localisation and stability. This reveals that while cadherin 2 is expressed across the entire tissue, functional apical junctions are first assembled in the transition zone and become progressively more stable across the leader-follower axis of the tissue. Perturbation experiments demonstrate that the formation of these apical adherens junctions requires dynamic microtubules. However, once stabilised, adherens junction maintenance is microtubule independent. Combined, these data identify a mechanism for regulating leader-to-follower transitions within migrating collectives, based on the relocation and stabilisation of cadherins, and reveal a key role for dynamic microtubules in this process.

Intravascular migration of a broken cerclage wire into the left heart.

PubMed

Leonardi, Francesco; Rivera, Fabrizio

2014-10-01

This article describes a patient in whom a broken cerclage wire migrated from the left hip into the left ventricle. A 71-year-old woman was admitted to the authors' hospital for preoperative examination before femoral hernia repair. Chest radiograph showed a metallic wire in the left ventricle. Twenty-four years earlier, she had a revision arthroplasty. During revision surgery, fragments of the osteotomy were fixed to the femur with multiple cerclage wires. During the past 5 years, radiographic follow-up showed progressive multiple ruptures of cerclage wires. The cerclage wiring was not removed because the patient had no related clinical symptoms. Radiograph of the left hip showed a well-fixed cemented acetabular ring and an uncemented femoral stem with a healed trochanteric osteotomy. All cerclage wires were broken into multiple parts, and it was very difficult to determine which part had migrated into the heart. Thoracic computed tomography scan showed wire that had migrated into the anterior left ventricular myocardial wall at the atrioventricular level. The patient had no clinical symptoms. Electrocardiogram showed a normal sinus rhythm and right bundle branch block. Because of the high risk of surgical left ventriculotomy associated with searching for wire that had migrated into the myocardial wall, patient monitoring was planned. Definitive management of this complication constitutes a dilemma. Although this complication is highly unusual, the possibility of intracardiac migration of broken wire should be considered when deciding on prophylactic surgical removal of hardware after fracture or osteotomy healing. Copyright 2014, SLACK Incorporated.

Quarterly Progress Report (January 1 to March 31, 1950)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brookhaven National Laboratory

This is the first of a series of Quarterly Reports. These reports will deal primarily with the progress made in our scientific program during a three months period. Those interested in matters pertaining to organization, administration, complete scientific program, personnel and other matters not directly involved in current scientific progress are referred to our Annual Progress Report which is issued in January. We have attempted to describe new information that appears significant, or of interest, to other scientists within the Atomic Energy Commission Laboratories. No effort has been made, however, to detail progress in each and every research project. Littlemore » or no reference will therefore be found to the projects in which progress during the current period is considered too inconclusive. Since our organizational structure is departmental, the work described herein is arranged in the following sequence: (1) Accelerator Project; (2) Biology Department; (3) Chemistry Department; (4) Instrumentation and Health Physic8 Department; (5) Medical Department; (6) Physics Department; and (7) Reactor Science and Engineering Department.« less

Walla Walla River Fish Passage Operations Program, 2004-2005 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bronson, James P.; Duke, Bill B.

2006-02-01

In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survivalmore » of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2004-2005 project year, there were 590 adult summer steelhead, 31 summer steelhead kelts (Oncorhynchus mykiss), 70 adult bull trout (Salvelinus confluentus); 80 adult and 1 jack spring Chinook (O. tshawytscha) enumerated at the Nursery Bridge Dam fishway video counting window between December 13, 2004, and June 16, 2005. Summer steelhead and spring chinook were observed moving upstream while bull trout were observed moving both upstream and downstream of the facility. In addition, the old ladder trap was operated by ODFW in order to enumerate fish passage. Of the total, 143 adult summer steelhead and 15 summer steelhead kelts were enumerated at the west ladder at Nursery Bridge Dam during the video efforts between February 4 and May 23, 2005. Operation of the Little Walla Walla River juvenile trap for trap and haul purposes was not necessary this year.« less

Cell Migration in 1D and 2D Nanofiber Microenvironments.

PubMed

Estabridis, Horacio M; Jana, Aniket; Nain, Amrinder; Odde, David J

2018-03-01

Understanding how cells migrate in fibrous environments is important in wound healing, immune function, and cancer progression. A key question is how fiber orientation and network geometry influence cell movement. Here we describe a quantitative, modeling-based approach toward identifying the mechanisms by which cells migrate in fibrous geometries having well controlled orientation. Specifically, U251 glioblastoma cells were seeded onto non-electrospinning Spinneret based tunable engineering parameters fiber substrates that consist of networks of suspended 400 nm diameter nanofibers. Cells were classified based on the local fiber geometry and cell migration dynamics observed by light microscopy. Cells were found in three distinct geometries: adhering two a single fiber, adhering to two parallel fibers, and adhering to a network of orthogonal fibers. Cells adhering to a single fiber or two parallel fibers can only move in one dimension along the fiber axis, whereas cells on a network of orthogonal fibers can move in two dimensions. We found that cells move faster and more persistently in 1D geometries than in 2D, with cell migration being faster on parallel fibers than on single fibers. To explain these behaviors mechanistically, we simulated cell migration in the three different geometries using a motor-clutch based model for cell traction forces. Using nearly identical parameter sets for each of the three cases, we found that the simulated cells naturally replicated the reduced migration in 2D relative to 1D geometries. In addition, the modestly faster 1D migration on parallel fibers relative to single fibers was captured using a correspondingly modest increase in the number of clutches to reflect increased surface area of adhesion on parallel fibers. Overall, the integrated modeling and experimental analysis shows that cell migration in response to varying fibrous geometries can be explained by a simple mechanical readout of geometry via a motor-clutch mechanism.

Communication and cultural interaction in health promotion strategies to migrant populations in Italy: the cross-cultural phone counselling experience.

PubMed

Taglieri, Filippo Maria; Colucci, Anna; Barbina, Donatella; Fanales-Belasio, Emanuele; Luzi, Anna Maria

2013-01-01

In the last 10 years migration processes have progressively increased worldwide and in Italy about 5 millions of residing migrants are estimated. To meet health needs of these new residents, effective relational and communication tools, which allow a reciprocal intercultural interaction within health care structures, are therefore necessary. This article faces the main features of the relational-communication processes associated with health promotion and care in the migrant population in Italy to the aim of identifying the key and critical points within the interaction between different cultures, focusing on the role of specific professional figures, including cultural mediators and health educators. Within the activity of HIV phone counselling operated by Psycho-sociobehavioural, Communication and Training Operating Unit of National Institute of Health in Italy, an intercultural approach was successfully experienced in a project targeted to migrants (2007-2008). Specifically, the presence of cultural mediators answering in the languages of main migrants' groups allowed the increase of calls from migrant people and of the information provided.

CENTRAL PLATEAU REMEDIATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

ROMINE, L.D.

2006-02-01

A systematic approach to closure planning is being implemented at the Hanford Site's Central Plateau to help achieve the goal of closure by the year 2035. The overall objective of Central Plateau remediation is to protect human health and the environment from the significant quantity of contaminated material that resulted from decades of plutonium production in support of the nation's defense. This goal will be achieved either by removing contaminants or placing the residual contaminated materials in a secure configuration that minimizes further migration to the groundwater and reduces the potential for inadvertent intrusion into contaminated sites. The approach tomore » Central Plateau cleanup used three key concepts--closure zones, closure elements, and closure process steps--to create an organized picture of actions required to complete remediation. These actions were merged with logic ties, constraints, and required resources to produce an integrated time-phased schedule and cost profile for Central Plateau closure. Programmatic risks associated with implementation of Central Plateau closure were identified and analyzed. Actions to mitigate the most significant risks are underway while high priority remediation projects continue to make progress.« less

Theoretical studies on the mechanical behavior of granular materials under very low intergranular stresses

NASA Technical Reports Server (NTRS)

French, Kenneth W., Jr.

1986-01-01

The salient aspects of the theoretical modeling of a conventional triaxial test (CTC) of a cohesionless granular medium with stress and strain rate loading are described. Included are a controllable gravitational body force and provision for low confining pressure and/or very low intergranular stress. The modeling includes rational, analytic, and numerical phases, all in various stages of development. The numerical evolutions of theoretical models will be used in final design stages and in the analysis of the experimental data. In this the experimental design stage, it is of special interest to include in the candidate considerations every anomaly found in preliminary terrestrial experimentation. Most of the anomalies will be eliminated by design or enhanced for measurement as the project progresses. The main aspect of design being not the physical apparatus but the type and trajectories of loading elected. The major considerations that have been treated are: appearance and growth of local surface aberrations, stress-power coefficients, strain types, optical strain, radial bead migration, and measures of rotation for the proper stress flux.

MIGRESIVES: a research project on migration from adhesives in food-packaging materials in support of European legislation and standardization.

PubMed

Störmer, A; Franz, R

2009-12-01

Most food packages and food-contact materials are manufactured using adhesives. The European Union regulates all food-contact materials, as their constituents may not contaminate food and endanger consumers' health. In contrast to plastics which are regulated by positive lists of authorized ingredients, adhesives have not yet a specific regulation. The MIGRESIVES project aimed to elaborate a scientific global risk-assessment approach to meet current general European Union regulatory requirements and as a basis for future specific European Union legislation as well as to provide the industry, especially small and medium-sized enterprises, a tool to ensure that migration from adhesives is in compliance with the regulatory requirements. The idea was to demonstrate that consumers' exposure to chemicals released by adhesives is in many cases below levels of concern. Technical/scientific knowledge from industry and research institutes will be merged into a collective research endeavour gathering all stakeholders. The major milestones are (1) the classification of adhesives according to chemistry and uses, (2) the test strategies based on physico-chemical behaviour of adhesives, (3) modelling migration/exposure from adhesives, (4) providing guidelines to integrate the risk-assessment approach into the daily life of companies, (5) the feasibility of applying the toxicological approach from the European Union BIOSAFEPAPER project, and (6) extensive training/education to small and medium-sized enterprises (SMEs) and large dissemination for general adoption of the concept in Europe.

Still on the Margins: Migration, English Language Learning, and Mental Health in Immigrant Psychiatric Patients

ERIC Educational Resources Information Center

Eamer, Allyson; Fernando, Shanti; King, Alyson E.

2017-01-01

This qualitative study explores the reflexive relationships among mental illness, acculturation, and progress toward English proficiency in five adult immigrants being treated at a Canadian psychiatric hospital. The research explores the additional challenges faced by mentally ill individuals when learning a new language and the extent to which…

Apo-10'-lycopenoic acid inhibits cancer cell migration and angiogenesis and induces peroxisome proliferator-activated receptor gamma

USDA-ARS?s Scientific Manuscript database

Scope: We have previously shown that apo-10'-lycopenoic acid (ALA), a derivative of lycopene through cleavage by carotene-9',10'-oxygenase, inhibits tumor progression and metastasis in both liver and lung cancer animal models. The underlying mechanism remains unknown. We hypothesized that ALA inhibi...

Asian migration to Australia: food and health consequences.

PubMed

Wahlqvist, Mark L

2002-01-01

Australia's food and health patterns are inextricably and increasingly linked with Asia. Indigenous Australians arrived in the continent via Asia and have linguistic connections with people who settled in south India; there was interaction and food trade between both South-East Asia and China and northern indigenous Australians over thousands of years. After European settlement in 1788, there have been several and increasing (apart from the period of the infamous White Australian Policy following the Colonial period and Independence, with Federation, in 1901) waves of Asian migration, notably during the gold rush (Chinese), the building of the overland Telegraph (Afghans), the Colombo Plan and Asian student education in Australia from the 1950s onwards (South-Eeast Asians), and with refugees (Vietnamese and mainland Chinese), and business (late twentieth century) and progressive family reunion. Each wave has injected additional food cultural elements and caused a measure of health change for migrants and host citizens. Of principal advantage to Australia has been the progressive diversification of the food supply and associated health protection. This has increased food security and sustainability. The process of Australian eating patterns becoming Asianized is evident through market garden development (and the introduction of new foods), fresh food markets and groceries, restaurants and the development of household cooking skills (often taught by student boarders). Most of the diversification has been with grain (rice), legumes (soy), greens, root vegetables, and various 'exotic fruits'. Food acculturation with migration is generally bi-directional. Thus, for Asians in Australia, there has been a decrease in energy expenditure (and a lower plane of energy throughput), an increase in food energy density (through increased fat and sugary drink intakes), and a decrease in certain health protective foods (lentils, soy, greens) and beverages (tea). This sets the stage for 'eco-diseases'. In a population probably genetically programmed (but modifiably) in utero to abdominal obesity, diabetes (type II and gestational) and cardiovascular disease, these conditions may be rapidly acquired on migration, along with certain cancers (breast, colo-rectal and prostate). Thus, whilst Asian migration to Australia has provided health opportunities for host citizens, there have been threats to migrant citizens in regard to nutrition-related health.

Overexpression of SAMD9 suppresses tumorigenesis and progression during non small cell lung cancer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, Qing; Yu, Tao; Ren, Yao-Yao

2014-11-07

Highlights: • SAMD9 is down-regulated in human non-small cell lung cancer (NSCLC). • Knockdown of SAMD9 expression is increased the invasion, migration and proliferation in H1299 cells in vitro. • Overexpression of SAMD9 suppressed proliferation and invasion in A549 cells in vitro. • Depletion of SAMD9 increases tumor formation in vivo. - Abstract: The Sterile Alpha Motif Domain-containing 9 (SAMD9) gene has been recently emphasized during the discovery that it is expressed at a lower level in aggressive fibromatosis and some cases of breast and colon cancer, however, the underlying mechanisms are poorly understood. Here, we found that SAMD9 ismore » down-regulated in human non-small cell lung cancer (NSCLC). Furthermore, knockdown of SAMD9 expression is increased the invasion, migration and proliferation in H1299 cells in vitro and overexpression of SAMD9 suppressed proliferation and invasion in A549 cells. Finally, depletion of SAMD9 increases tumor formation in vivo. Our results may provide a strategy for blocking NSCLC tumorigenesis and progression.« less

Cerebral Toxocariasis: Silent Progression to Neurodegenerative Disorders?

PubMed Central

Holland, Celia V.; Loxton, Karen; Barghouth, Ursula

2015-01-01

SUMMARY Toxocara canis and T. cati are highly prevalent nematode infections of the intestines of dogs and cats. In paratenic hosts, larvae do not mature in the intestine but instead migrate through the somatic tissues and organs of the body. The presence of these migrating larvae can contribute to pathology. Toxocara larvae can invade the brains of humans, and while case descriptions of cerebral toxocariasis are historically rare, improved diagnosis and greater awareness have contributed to increased detection. Despite this, cerebral or neurological toxocariasis (NT) remains a poorly understood phenomenon. Furthermore, our understanding of cognitive deficits due to toxocariasis in human populations remains particularly deficient. Recent data describe an enhanced expression of biomarkers associated with brain injury, such as GFAP, AβPP, transforming growth factor β1 (TGF-β1), NF-L, S100B, tTG, and p-tau, in mice receiving even low doses of Toxocara ova. Finally, this review outlines a hypothesis to explore the relationship between the presence of T. canis larvae in the brain and the progression of Alzheimer's disease (AD) due to enhanced AD-associated neurodegenerative biomarker expression. PMID:26062575

Gamma-enolase: a well-known tumour marker, with a less-known role in cancer

PubMed Central

Vizin, Tjasa; Kos, Janko

2015-01-01

Background Gamma-enolase, known also as neuron-specific enolase (NSE), is an enzyme of the glycolytic pathway, which is expressed predominantly in neurons and cells of the neuroendocrine system. As a tumour marker it is used in diagnosis and prognosis of cancer; however, the mechanisms enrolling it in malignant progression remain elusive. As a cytoplasmic enzyme gamma-enolase is involved in increased aerobic glycolysis, the main source of energy in cancer cells, supporting cell proliferation. However, different cellular localisation at pathophysiological conditions, proposes other cellular engagements. Conclusions The C-terminal part of the molecule, which is not related to glycolytic pathway, was shown to promote survival of neuronal cells by regulating neuronal growth factor receptor dependent signalling pathways, resulting also in extensive actin cytoskeleton remodelling. This additional function could be important also in cancer cells either to protect cells from stressful conditions and therapeutic agents or to promote tumour cell migration and invasion. Gamma-enolase might therefore have a multifunctional role in cancer progression: it supports increased tumour cell metabolic demands, protects tumour cells from stressful conditions and promotes their invasion and migration. PMID:26401126

Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway

PubMed Central

Jeong, Ae Lee; Han, Sora; Lee, Sunyi; Su Park, Jeong; Lu, Yiling; Yu, Shuangxing; Li, Jane; Chun, Kyung-Hee; Mills, Gordon B.; Yang, Young

2016-01-01

Mutation of PPP2R1A has been observed at high frequency in endometrial serous carcinomas but at low frequency in ovarian clear cell carcinoma. However, the biological role of mutation of PPP2R1A in ovarian and endometrial cancer progression remains unclear. In this study, we found that PPP2R1A expression is elevated in high-grade primary tumor patients with papillary serous tumors of the ovary. To determine whether increased levels or mutation of PPP2R1A might contribute to cancer progression, the effects of overexpression or mutation of PPP2R1A on cell proliferation, migration, and PP2A phosphatase activity were investigated using ovarian and endometrial cancer cell lines. Among the mutations, PPP2R1A-W257G enhanced cell migration in vitro through activating SRC-JNK-c-Jun pathway. Overexpression of wild type (WT) PPP2R1A increased its binding ability with B56 regulatory subunits, whereas PPP2R1A-mutations lost the ability to bind to most B56 subunits except B56δ. Total PP2A activity and PPP2R1A-associated PP2Ac activity were significantly increased in cells overexpressing PPP2R1A-WT. In addition, overexpression of PPP2R1A-WT increased cell proliferation in vitro and tumor growth in vivo. PMID:27272709

STIM1 Overexpression Promotes Colorectal Cancer Progression, Cell Motility and COX-2 Expression

PubMed Central

Wang, Jaw-Yuan; Sun, Jianwei; Huang, Ming-Yii; Wang, Yu-Shiuan; Hou, Ming-Feng; Sun, Yan; He, Huifang; Krishna, Niveditha; Chiu, Siou-Jin; Lin, Shengchen; Yang, Shengyu; Chang, Wei-Chiao

2014-01-01

Tumor metastasis is the major cause of death among cancer patients, with more than 90% of cancer-related death attributable to the spreading of metastatic cells to secondary organs. Store-operated Ca2+ entry (SOCE) is the predominant Ca2+ entry mechanism in most cancer cells, and STIM1 is the endoplasmic reticulum (ER) Ca2+ sensor for store-operated channels (SOC). Here we reported that the STIM1 was overexpressed in colorectal cancer (CRC) patients. STIM1 overexpression in CRC was significantly associated with tumor size, depth of invasion, lymphnode metastasis status and serum levels of carcinoembryonic antigen. Furthermore, ectopic expression of STIM1 promoted CRC cell motility, while depletion of STIM1 with shRNA inhibited CRC cell migration. Our data further suggested that STIM1 promoted CRC cell migration through increasing the expression of cyclooxygenase-2 (COX-2) and production of prostaglandin E2 (PGE2). Importantly, ectopically expressed COX-2 or exogenous PGE2 were able to rescue migration defect in STIM1 knockdown CRC cells, and inhibition of COX-2 with ibuprofen and indomethacin abrogated STIM1-mediated CRC cell motility. In short, our data provided clinicopathological significance for STIM1 and store-operated Ca2+ entry in CRC progression, and implicated a role for COX-2 in STIM1-mediated CRC metastasis. Our studies also suggested a new approach to inhibit STIM1-mediated metastasis with COX-2 inhibitors. PMID:25381814

Matrix metalloproteinase-9 is up-regulated by CCL21/CCR7 interaction via extracellular signal-regulated kinase-1/2 signaling and is involved in CCL21-driven B-cell chronic lymphocytic leukemia cell invasion and migration.

PubMed

Redondo-Muñoz, Javier; José Terol, María; García-Marco, José A; García-Pardo, Angeles

2008-01-01

B-cell chronic lymphocytic leukemia (B-CLL) progression is frequently accompanied by clinical lymphadenopathy, and the CCL21 chemokine may play an important role in this process. Indeed, CCR7 (the CCL21 receptor), as well as matrix metalloproteinase-9 (MMP-9), are overexpressed in infiltrating B-CLL cells. We have studied whether MMP-9 is regulated by CCL21 and participates in CCL21-dependent migration. CCL21 significantly increased B-CLL MMP-9 production, measured by gelatin zymography. This was inhibited by blocking extracellular signal-regulated kinase-1/2 (ERK1/2) activity or by cell transfection with CCR7-siRNA. Accordingly, CCL21/CCR7 interaction activated the ERK1/2/c-Fos pathway and increased MMP-9 mRNA. CCL21-driven B-CLL cell migration through Matrigel or human umbilical vein endothelial cells (HUVEC) was blocked by anti-CCR7 antibodies, CCR7-siRNA transfection, or the ERK1/2 inhibitor U0126, as well as by anti-MMP-9 antibodies or tissue inhibitor of metalloproteinase 1 (TIMP-1). These results strongly suggest that MMP-9 is involved in B-CLL nodal infiltration and expand the roles of MMP-9 and CCR7 in B-CLL progression. Both molecules could thus constitute therapeutic targets for this disease.

Cutaneous antigen-stimulating lymphokine production by lymphocytes of patients with progressive systemic sclerosis (scleroderma).

PubMed Central

Kondo, H; Rabin, B S; Rodnan, G P

1976-01-01

Cell-mediated immunity to skin extracts was studied by the macrophage migration inhibition test, lymphocyte transformation, and direct cytotoxicity to skin fibroblasts, in normal individuals and patients with progressive systemic sclerosis. The latter included 18 individuals with diffuse scleroderma and 12 with the CREST syndrome, a variant form of systemic sclerosis in which there is more limited involvement of the skin. Controls consisted of 13 patients with other connective tissue diseases and 16 normal individuals. Phosphate-buffered saline and 3 M KCl extracts of both normal and sclerodermatous skin were used as antigens. No evidence of lymphocyte reactivity was found by the lymphocyte transformation and direct cytotoxicity test procedures. However, the lymphocytes of patients with diffuse scleroderma did respond to extracts of both normal and sclerodermatous skin in the migration inhibition assay. 10 of 16 patients (62.5%) had migration indices below 2 SD of the normal range, 1 of 10 CREST patients and 1 of 13 patients with other connective tissue diseases showed similar reactivity. Antisera specific for immunoglobulin-bearing lymphocytes (B lymphocytes) and T lymphocytes were used to characterize the lymphocytes found in skin biopsies of patients with diffuse scleroderma. T lymphocytes made up the majority of lymphocytes in the skin infiltrates. These findings suggest that lymphocytes sensitized to skin extracts are present in patients with diffuse scleroderma. The cell-mediated immune reaction to skin antigens may be a factor in the pathogenesis of diffuse scleroderma. Images PMID:791970

Processing and thermodynamics research, Volume II. Monthly progress report, November 1984

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Data assembly is in progress on the Venezuelan Cerro Negro, California, Wilmington and Mexican Mayan crudes. A draft of a proposal is in preparation that might join this correlational work with complementary correlational work at the University of Oklahoma (Project BPT1). Cerro Negro 200 to 425/sup 0/C and 425 to 550/sup 0/C saturate fractions were analyzed with the CEC 21-103 mass spectrometer using ASTM method D2786, and experiments were devised to establish reproducibility of analyses with the KRATOS MS-50 (Project BPT2). Thermodynamic property measurements on organic nitrogen compounds included a liquid phase heat capacity study of 2,5-dimethylpyridine, and the oxygenmore » sensitivity of phenanthridine was checked prior to bomb calorimetry (Project BPT3A). Heat capacity measurements on chroman are progressing well, and vapor pressure measurements of tetrahydrophenanthrene are in progress (Project BPT3B). Work was begun on the design and procurement of equipment necessary for construction of a bench scale catalytic cracking unit. The supercritical extraction unit is being assembled (Project OPT1). Thiopene separations were begun on Cerro Negro 425 to 550/sup 0/C neutrals, and separation of sulfides from Cerro Negro 550 to 700/sup 0/C and 700+/sup 0/C neutrals was completed (Project OPT2). Preparations for PVT studies of methanol are nearing completion, and a list of critically needed PVT measurements was reviewed to help select the next compound for study. A possible candidate is hydrogen sulfide. (Project OPT3). Good progress continues in the treatment of the RP feed (Project OPT4) to recover Milspec F-76. It is anticipated that 85% recovery is possible by the chemical treatment/distillation process.« less

Long non-coding RNA AFAP1-antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival.

PubMed

Zhao, Huazhou; Zhang, Kecheng; Wang, Ting; Cui, Jianxin; Xi, Hongqing; Wang, Yi; Song, Yanjing; Zhao, Xudong; Wei, Bo; Chen, Lin

2018-06-01

Gastric cancer (GC) is the second-leading cause of cancer-associated mortality worldwide. AFAP1-antisense RNA 1 (AFAP1-AS1), a long non-coding RNA (lncRNA), is believed to promote the aggressive progression of cancer; however, its role in GC remains largely unknown. In the present study, the expression of AFAP1-AS1 in GC tissues and cell lines was measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Knockdown of AFAP1-AS1 was performed using a lentiviral vector containing a short hairpin RNA. The proliferation of GC cells was measured using Cell Counting kit-8. The migration and invasion of GC cells were analyzed using a QCM Laminin Migration Assay kit and a Cell Invasion Assay kit. The levels of epithelial-mesenchymal transition (EMT)-associated proteins were detected by western blot analysis. The cut-off value of the expression of AFAP1-AS1 was evaluated using receiver operating characteristic (ROC) curves and patient survival rate was analyzed using Kaplan-Meier. The expression of AFAP1-AS1 was significantly increased in the primary tumor tissues of GC patients with lymph node metastasis or tumor node metastasis stage (stage III or IV; P<0.01). ROC curve analysis revealed that the expression of AFAP-AS1, at a cut-off value of 0.5040, could distinguish GC tissues from the matched normal tissues, with an AUC of 0.8802, sensitivity of 81.25% and specificity of 83.75%. The overexpression of AFAP1-AS1 was positively associated with the poor survival rates of GC patients. Furthermore, the downregulation of AFAP1-AS1 significantly inhibited the proliferation, migration and invasion of GC cells in vitro (P<0.01). The decrease in AFAP1-AS1 expression significantly suppressed the expression level of N-cadherin protein in GC cells and increased that of E-cadherin. The present study demonstrated that the expression signature of AFAP1-AS1 may serve as a biomarker for the diagnosis and prognosis of GC, and its downregulation may repress the aggressive progression of GC, partially through inhibiting the EMT progress.

Transneuronal pathways to the vestibulocerebellum

NASA Technical Reports Server (NTRS)

Kaufman, G. D.; Mustari, M. J.; Miselis, R. R.; Perachio, A. A.

1996-01-01

The alpha-herpes virus (pseudorabies, PRV) was used to observe central nervous system (CNS) pathways associated with the vestibulocerebellar system. Retrograde transneuronal migration of alpha-herpes virions from specific lobules of the gerbil and rat vestibulo-cerebellar cortex was detected immunohistochemically. Using a time series analysis, progression of infection along polyneuronal cerebellar afferent pathways was examined. Pressure injections of > 20 nanoliters of a 10(8) plaque forming units (pfu) per ml solution of virus were sufficient to initiate an infectious locus which resulted in labeled neurons in the inferior olivary subnuclei, vestibular nuclei, and their afferent cell groups in a progressive temporal fashion and in growing complexity with increasing incubation time. We show that climbing fibers and some other cerebellar afferent fibers transported the virus retrogradely from the cerebellum within 24 hours. One to three days after cerebellar infection discrete cell groups were labeled and appropriate laterality within crossed projections was preserved. Subsequent nuclei labeled with PRV after infection of the flocculus/paraflocculus, or nodulus/uvula, included the following: vestibular (e.g., z) and inferior olivary nuclei (e.g., dorsal cap), accessory oculomotor (e.g., Darkschewitsch n.) and accessory optic related nuclei, (e.g., the nucleus of the optic tract, and the medial terminal nucleus); noradrenergic, raphe, and reticular cell groups (e.g., locus coeruleus, dorsal raphe, raphe pontis, and the lateral reticular tract); other vestibulocerebellum sites, the periaqueductal gray, substantia nigra, hippocampus, thalamus and hypothalamus, amygdala, septal nuclei, and the frontal, cingulate, entorhinal, perirhinal, and insular cortices. However, there were differences in the resulting labeling between infection in either region. Double-labeling experiments revealed that vestibular efferent neurons are located adjacent to, but are not included among, flocculus-projecting supragenual neurons. PRV transport from the vestibular labyrinth and cervical muscles also resulted in CNS infections. Virus propagation in situ provides specific connectivity information based on the functional transport across synapses. The findings support and extend anatomical data regarding vestibulo-olivo-cerebellar pathways.

THE PROJECT: an Observatory / Transport Spaceship for Discovering and Populating Habitable Extrasolar Terrestrial Planets

NASA Astrophysics Data System (ADS)

Kilston, S.

1998-12-01

Recent extrasolar planet discoveries and related progress in astrophysics have refined our knowledge of the implications of the Drake equation. The Space Interferometry Mission and the planned Terrestrial Planet Finder will deepen this understanding, and begin pointing the way to places we need to explore at closer range. If the correct resolution of the Fermi paradox regarding intelligent extraterrestrials (``where are they?") is found to lie in the actual scarcity of such beings, it may turn out that we are more advanced than most other life-forms in our galaxy. In this case, a main purpose in finding planets may be to find places for us to go: astronomy will once again play a major role in human navigation and migration. We describe a strawman design concept for an astronomical observatory ship designed for launch beyond our solar system within several hundred years. This ship design would employ plausible physics, biology, technology, sociology, and economics to carry one million passengers in a one-G environment shielded from space radiation. A cruising speed under 0.01 c, slower than in many science-fiction concepts, minimizes power requirements and the danger from collisional impacts. The ship would contain all subsystems needed to sustain multi-generational life on a voyage of thousands of years, as well as the observatories to identify for human settlement a habitable extrasolar planet. Even the modestly advanced technology described here could spread intelligent life throughout our galaxy within 40 million years, a very small fraction of the galaxy's age. Motivation for such an ambitious project is three-fold: expanding our knowledge of the universe, enlisting the efforts and enthusiasms of humankind toward a very grand goal which will stimulate progress in all aspects of our cultures and technologies, and participating in the process of spreading life so its survivability and fruition are enhanced.

GPER, IGF-IR, and EGFR transduction signaling are involved in stimulatory effects of zinc in breast cancer cells and cancer-associated fibroblasts.

PubMed

Pisano, Assunta; Santolla, Maria Francesca; De Francesco, Ernestina Marianna; De Marco, Paola; Rigiracciolo, Damiano Cosimo; Perri, Maria Grazia; Vivacqua, Adele; Abonante, Sergio; Cappello, Anna Rita; Dolce, Vincenza; Belfiore, Antonino; Maggiolini, Marcello; Lappano, Rosamaria

2017-02-01

Zinc (Zn) is an essential trace mineral that contributes to the regulation of several cellular functions; however, it may be also implicated in the progression of breast cancer through different mechanisms. It has been largely reported that the classical estrogen receptor (ER), as well as the G protein estrogen receptor (GPER, previously known as GPR30) can exert a main role in the development of breast tumors. In the present study, we demonstrate that zinc chloride (ZnCl 2 ) involves GPER in the activation of insulin-like growth factor receptor I (IGF-IR)/epidermal growth factor receptor (EGFR)-mediated signaling, which in turn triggers downstream pathways like ERK and AKT in breast cancer cells, and main components of the tumor microenvironment namely cancer-associated fibroblasts (CAFs). Further corroborating these findings, ZnCl 2 stimulates a functional crosstalk of GPER with IGF-IR and EGFR toward the transcription of diverse GPER target genes. Then, we show that GPER contributes to the stimulatory effects induced by ZnCl 2 on cell-cycle progression, proliferation, and migration of breast cancer cells as well as migration of CAFs. Together, our data provide novel insights into the molecular mechanisms through which zinc may exert stimulatory effects in breast cancer cells and CAFs toward tumor progression. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Progressive Design of Plasmonic Metal-Semiconductor Ensemble toward Regulated Charge Flow and Improved Vis-NIR-Driven Solar-to-Chemical Conversion.

PubMed

Han, Chuang; Quan, Quan; Chen, Hao Ming; Sun, Yugang; Xu, Yi-Jun

2017-04-01

Surface plasmon resonance (SPR)-mediated photocatalysis without the bandgap limitations of traditional semiconductor has aroused significant attention in solar-to-chemical energy conversion. However, the photocatalytic efficiency barely initiated by the SPR effects is still challenged by the low concentration and ineffective extraction of energetic hot electrons, slow charge migration rates, random charge diffusion directions, and the lack of highly active sites for redox reactions. Here, the tunable, progressive harvesting of visible-to-near infrared light (vis-NIR, λ > 570 nm) by designing plasmonic Au nanorods and metal (Au, Ag, or Pt) nanoparticle codecorated 1D CdS nanowire (1D CdS NW) ensemble is reported. The intimate integration of these metal nanostructures with 1D CdS NWs promotes the extraction and manipulated directional separation and migration of hot charge carriers in a more effective manner. Such cooperative synergy with tunable control of interfacial interaction, morphology optimization, and cocatalyst strategy results in the distinctly boosted performance for vis-NIR-driven plasmonic photocatalysis. This work highlights the significance of rationally progressive design of plasmonic metal-semiconductor-based composite system for boosting the regulated directional flow of hot charge carrier and thus the more efficient use of broad-spectrum solar energy conversion. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Connective tissue growth factor (CTGF) and cancer progression.

PubMed

Chu, Chia-Yu; Chang, Cheng-Chi; Prakash, Ekambaranellore; Kuo, Min-Liang

2008-11-01

Connective tissue growth factor (CTGF) is a member of the CCN family of secreted, matrix-associated proteins encoded by immediate early genes that play various roles in angiogenesis and tumor growth. CCN family proteins share uniform modular structure which mediates various cellular functions such as regulation of cell division, chemotaxis, apoptosis, adhesion, motility, angiogenesis, neoplastic transformation, and ion transport. Recently, CTGF expression has been shown to be associated with tumor development and progression. There is growing body of evidence that CTGF may regulate cancer cell migration, invasion, angiogenesis, and anoikis. In this review, we will highlight the influence of CTGF expression on the biological behavior and progression of various cancer cells, as well as its regulation on various types of protein signals and their mechanisms.

LPA Induces Colon Cancer Cell Proliferation through a Cooperation between the ROCK and STAT-3 Pathways.

PubMed

Leve, Fernanda; Peres-Moreira, Rubem J; Binato, Renata; Abdelhay, Eliana; Morgado-Díaz, José A

2015-01-01

Lysophosphatidic acid (LPA) plays a critical role in the proliferation and migration of colon cancer cells; however, the downstream signaling events underlying these processes remain poorly characterized. The aim of this study was to investigate the signaling pathways triggered by LPA to regulate the mechanisms involved in the progression of colorectal cancer (CRC). We have used three cell line models of CRC, and initially analyzed the expression profile of LPA receptors (LPAR). Then, we treated the cells with LPA and events related to their tumorigenic potential, such as migration, invasion, anchorage-independent growth, proliferation as well as apoptosis and cell cycle were evaluated. We used the Chip array technique to analyze the global gene expression profiling that occurs after LPA treatment, and we identified cell signaling pathways related to the cell cycle. The inhibition of these pathways verified the conclusions of the transcriptomic analysis. We found that the cell lines expressed LPAR1, -2 and -3 in a differential manner and that 10 μM LPA did not affect cell migration, invasion and anchorage-independent growth, but it did induce proliferation and cell cycle progression in HCT-116 cells. Although LPA in this concentration did not induce transcriptional activity of β-catenin, it promoted the activation of Rho and STAT-3. Moreover, ROCK and STAT-3 inhibitors prevented LPA-induced proliferation, but ROCK inhibition did not prevent STAT-3 activation. Finally, we observed that LPA regulates the expression of genes related to the cell cycle and that the combined inhibition of ROCK and STAT-3 prevented cell cycle progression and increased the LPA-induced expression of cyclins E1, A2 and B1 to a greater degree than either inhibitor alone. Overall, these results demonstrate that LPA increases the proliferative potential of colon adenocarcinoma HCT-116 cells through a mechanism involving cooperation between the Rho-ROCK and STAT3 pathways involved in cell cycle control.

Long non-coding RNA BRAF-regulated lncRNA 1 promotes lymph node invasion, metastasis and proliferation, and predicts poor prognosis in breast cancer.

PubMed

Jiang, Jing; Shi, Sheng-Hong; Li, Xu-Jun; Sun, Long; Ge, Qi-Dong; Li, Chao; Zhang, Wei

2018-06-01

Long non-coding RNAs (lncRNAs) are primary regulators of cancer development via their involvement in almost every aspect of cell biology. Recent studies have indicated that lncRNAs serve pivotal roles in breast cancer (BC) progression; however, to the best of our knowledge, the role of the lncRNA BRAF-regulated lncRNA 1 (BANCR) in BC has not yet been elucidated. The present study revealed that BANCR was overexpressed in BC cell lines and tissues, and could promote the clinical progression of disease, including increases in tumor size, lymph node metastasis and Tumor-Node-Metastasis stage. Furthermore, high BANCR expression was demonstrated to be associated with poor overall survival rates and early recurrence of BC in patients. Additionally, univariate and multivariate COX regression analyses identified high BANCR expression as an independent risk factor of poor prognosis of patients with BC. In addition, to verify the function of BANCR in BC cell lines, BANCR expression was silenced using short hairpin RNAs in MDA-MB-231 cells and overexpressed in MDA-MB-468 cells. An MTT assay and colony formation assay indicated that BANCR knockdown could suppress the proliferation of BC cells, whereas BANCR upregulation induced the proliferation of BC cells. Furthermore, BANCR silencing also reduced the migration and invasion of BC cells, as demonstrated via transwell migration and invasion assays. Consistently, the migration and invasion of BC cells increased upon BANCR ectopic overexpression in MDA-MB-468 cells. Mechanistically, matrix metallopeptidase 2/9 and epithelial-mesenchymal transition markers may be the potential targets of BANCR in regulating BC metastasis. In conclusion, BANCR overexpression could promote the clinical progression, metastasis and proliferation of BC and indicate poor prognosis of patients with BC. BANCR may therefore be a potential prognostic marker and therapeutic target of patients with BC.

Verminous (Strongylus vulgaris) myelitis in a donkey.

PubMed

Mayhew, I G; Brewer, B D; Reinhard, M K; Greiner, E C

1984-01-01

A fifth stage Strongylus vulgaris migrated through the spinal cord of a 2-year-old, male donkey resulting in progressive paraparesis and then tetraplegia. A profound neutrophilic pleocytosis was detected on analysis of cerebrospinal fluid. The parasite appeared to have entered the mid-lumbar spinal cord, migrated to the cranial thoracic segments, exited, then re-entered the spinal cord a few segments craniad. It then traveled further cranially and was found in the third cervical spinal cord segment. Some parts of the lesion were remarkably free from tissue necrosis, hemorrhage and inflammation. Severe granulomatous myelitis with hemorrhage and necrosis were seen at other sites. The latter were quite similar to lesions seen in equine protozoal myeloencephalitis.