Selecting Power-Efficient Signal Features for a Low-Power Fall Detector.

PubMed

Wang, Changhong; Redmond, Stephen J; Lu, Wei; Stevens, Michael C; Lord, Stephen R; Lovell, Nigel H

2017-11-01

Falls are a serious threat to the health of older people. A wearable fall detector can automatically detect the occurrence of a fall and alert a caregiver or an emergency response service so they may deliver immediate assistance, improving the chances of recovering from fall-related injuries. One constraint of such a wearable technology is its limited battery life. Thus, minimization of power consumption is an important design concern, all the while maintaining satisfactory accuracy of the fall detection algorithms implemented on the wearable device. This paper proposes an approach for selecting power-efficient signal features such that the minimum desirable fall detection accuracy is assured. Using data collected in simulated falls, simulated activities of daily living, and real free-living trials, all using young volunteers, the proposed approach selects four features from a set of ten commonly used features, providing a power saving of 75.3%, while limiting the error rate of a binary classification decision tree fall detection algorithm to 7.1%.Falls are a serious threat to the health of older people. A wearable fall detector can automatically detect the occurrence of a fall and alert a caregiver or an emergency response service so they may deliver immediate assistance, improving the chances of recovering from fall-related injuries. One constraint of such a wearable technology is its limited battery life. Thus, minimization of power consumption is an important design concern, all the while maintaining satisfactory accuracy of the fall detection algorithms implemented on the wearable device. This paper proposes an approach for selecting power-efficient signal features such that the minimum desirable fall detection accuracy is assured. Using data collected in simulated falls, simulated activities of daily living, and real free-living trials, all using young volunteers, the proposed approach selects four features from a set of ten commonly used features, providing a power saving of 75.3%, while limiting the error rate of a binary classification decision tree fall detection algorithm to 7.1%.

Improved performance comparisons of radioxenon systems for low level releases in nuclear explosion monitoring.

PubMed

Haas, Derek A; Eslinger, Paul W; Bowyer, Theodore W; Cameron, Ian M; Hayes, James C; Lowrey, Justin D; Miley, Harry S

2017-11-01

The Comprehensive Nuclear-Test-Ban Treaty bans all nuclear tests and mandates development of verification measures to detect treaty violations. One verification measure is detection of radioactive xenon isotopes produced in the fission of actinides. The International Monitoring System (IMS) currently deploys automated radioxenon systems that can detect four radioxenon isotopes. Radioxenon systems with lower detection limits are currently in development. Historically, the sensitivity of radioxenon systems was measured by the minimum detectable concentration for each isotope. In this paper we analyze the response of radioxenon systems using rigorous metrics in conjunction with hypothetical representative releases indicative of an underground nuclear explosion instead of using only minimum detectable concentrations. Our analyses incorporate the impact of potential spectral interferences on detection limits and the importance of measuring isotopic ratios of the relevant radioxenon isotopes in order to improve discrimination from background sources particularly for low-level releases. To provide a sufficient data set for analysis, hypothetical representative releases are simulated every day from the same location for an entire year. The performance of three types of samplers are evaluated assuming they are located at 15 IMS radionuclide stations in the region of the release point. The performance of two IMS-deployed samplers and a next-generation system is compared with proposed metrics for detection and discrimination using representative releases from the nuclear test site used by the Democratic People's Republic of Korea. Copyright © 2017 Elsevier Ltd. All rights reserved.

High sensitivity detection of trace gases at atmospheric pressure using tunable diode lasers

NASA Technical Reports Server (NTRS)

Reid, J.; Sinclair, R. L.; Grant, W. B.; Menzies, R. T.

1985-01-01

A detailed study of the detection of trace gases at atmospheric pressure using tunable diode lasers is described. The influence of multipass cells, retroreflectors and topographical targets is examined. The minimum detectable infrared absorption ranges from 0.1 percent for a pathlength of 1.2 km to 0.01 percent over short pathlengths. The factors which limit this sensitivity are discussed, and the techniques are illustrated by monitoring atmospehric CO2 and CH4.

Ammonia Optical Sensing by Microring Resonators.

PubMed

Passaro, Vittorio M N; Dell'Olio, Francesco; De Leonardis, Francesco

2007-11-15

A very compact (device area around 40 μm²) optical ammonia sensor based on amicroring resonator is presented in this work. Silicon-on-insulator technology is used insensor design and a dye doped polymer is adopted as sensing material. The sensor exhibitsa very good linearity and a minimum detectable refractive index shift of sensing materialas low as 8x10 -5 , with a detection limit around 4 ‰.

Improved detection limits for electrospray ionization on a magnetic sector mass spectrometer by using an array detector.

PubMed

Cody, R B; Tamura, J; Finch, J W; Musselman, B D

1994-03-01

Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2∶1 for a 500 amol/μL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/μL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.

Synthesis of correlation filters: a generalized space-domain approach for improved filter characteristics

NASA Astrophysics Data System (ADS)

Sudharsanan, Subramania I.; Mahalanobis, Abhijit; Sundareshan, Malur K.

1990-12-01

Discrete frequency domain design of Minimum Average Correlation Energy filters for optical pattern recognition introduces an implementational limitation of circular correlation. An alternative methodology which uses space domain computations to overcome this problem is presented. The technique is generalized to construct an improved synthetic discriminant function which satisfies the conflicting requirements of reduced noise variance and sharp correlation peaks to facilitate ease of detection. A quantitative evaluation of the performance characteristics of the new filter is conducted and is shown to compare favorably with the well known Minimum Variance Synthetic Discriminant Function and the space domain Minimum Average Correlation Energy filter, which are special cases of the present design.

Probe-Specific Procedure to Estimate Sensitivity and Detection Limits for 19F Magnetic Resonance Imaging.

PubMed

Taylor, Alexander J; Granwehr, Josef; Lesbats, Clémentine; Krupa, James L; Six, Joseph S; Pavlovskaya, Galina E; Thomas, Neil R; Auer, Dorothee P; Meersmann, Thomas; Faas, Henryk M

2016-01-01

Due to low fluorine background signal in vivo, 19F is a good marker to study the fate of exogenous molecules by magnetic resonance imaging (MRI) using equilibrium nuclear spin polarization schemes. Since 19F MRI applications require high sensitivity, it can be important to assess experimental feasibility during the design stage already by estimating the minimum detectable fluorine concentration. Here we propose a simple method for the calibration of MRI hardware, providing sensitivity estimates for a given scanner and coil configuration. An experimental "calibration factor" to account for variations in coil configuration and hardware set-up is specified. Once it has been determined in a calibration experiment, the sensitivity of an experiment or, alternatively, the minimum number of required spins or the minimum marker concentration can be estimated without the need for a pilot experiment. The definition of this calibration factor is derived based on standard equations for the sensitivity in magnetic resonance, yet the method is not restricted by the limited validity of these equations, since additional instrument-dependent factors are implicitly included during calibration. The method is demonstrated using MR spectroscopy and imaging experiments with different 19F samples, both paramagnetically and susceptibility broadened, to approximate a range of realistic environments.

Improvements in an in vivo neutron activation analysis (NAA) method for the measurement of fluorine in human bone.

PubMed

Mostafaei, F; McNeill, F E; Chettle, D R; Prestwich, W V

2013-10-01

We previously published a method for the in vivo measurement of bone fluoride using neutron activation analysis (NAA) and demonstrated the utility of the technique in a pilot study of environmentally exposed people. The method involved activation of the hand in an irradiation cavity at the McMaster University Accelerator Laboratory and acquisition of the resultant γ-ray signals in a '4π' NaI(Tl) detector array of nine detectors. In this paper we describe a series of improvements to the method. This was investigated via measurement of hand simulating phantoms doped with varying levels of fluorine and fixed amounts of sodium, chlorine and calcium. Four improvements to the technique were tested since our first publication. The previously published detection limit for phantom measurements using this system was 0.66 mg F/g Ca. The accelerator irradiation and detection facilities were relocated to a new section of the laboratory and one more detector was added to the detection system. This was found to reduce the detection limit (possibly because of better detection shielding and additional detector) to 0.59 mg F/g Ca, a factor of 1.12. A new set of phantoms was developed and in this work we show that they improved the minimum detectable limit for fluoride in phantoms irradiated using neutrons produced by 2.15 MeV protons on lithium by a factor of 1.55. We compared the detection limits previously obtained using a summed signal from the nine detectors with the detection limit obtained by acquiring the spectra in anticoincidence mode for reduction of the disturbing signal from chlorine in bone. This was found to improve the ratio of the detection of fluorine to chlorine (an interfering signal) by a factor of 2.8 and the resultant minimum detection limit was found to be reduced by a factor of 1.2. We studied the effects of changing the timing of γ-ray acquisition. Our previously published data used a series of three 10 s acquisitions followed by a 300 s count. Changing the acquisition to a series of six 5 s acquisitions was found to further improve the detection limit by a factor of 1.4. We also present data showing that if the neutron dose is delivered to the phantom in a shorter time period, i.e. the dose rate is increased and irradiation shortened then the detection limit can be reduced by a further factor of 1.35.The overall improvement in detection limit by employing all of these changes was found to be a factor of 3.9. The technique now has an in phantom detection limit of 0.17 mg F/g Ca compared to a previous detection limit of 0.66 mg F/g Ca. The system can now be tested on human volunteers to see if individuals with diagnosed fluorosis can be distinguished from the general Canadian population using this technique.

Dual Energy Method for Breast Imaging: A Simulation Study.

PubMed

Koukou, V; Martini, N; Michail, C; Sotiropoulou, P; Fountzoula, C; Kalyvas, N; Kandarakis, I; Nikiforidis, G; Fountos, G

2015-01-01

Dual energy methods can suppress the contrast between adipose and glandular tissues in the breast and therefore enhance the visibility of calcifications. In this study, a dual energy method based on analytical modeling was developed for the detection of minimum microcalcification thickness. To this aim, a modified radiographic X-ray unit was considered, in order to overcome the limited kVp range of mammographic units used in previous DE studies, combined with a high resolution CMOS sensor (pixel size of 22.5 μm) for improved resolution. Various filter materials were examined based on their K-absorption edge. Hydroxyapatite (HAp) was used to simulate microcalcifications. The contrast to noise ratio (CNR tc ) of the subtracted images was calculated for both monoenergetic and polyenergetic X-ray beams. The optimum monoenergetic pair was 23/58 keV for the low and high energy, respectively, resulting in a minimum detectable microcalcification thickness of 100 μm. In the polyenergetic X-ray study, the optimal spectral combination was 40/70 kVp filtered with 100 μm cadmium and 1000 μm copper, respectively. In this case, the minimum detectable microcalcification thickness was 150 μm. The proposed dual energy method provides improved microcalcification detectability in breast imaging with mean glandular dose values within acceptable levels.

Dual Energy Method for Breast Imaging: A Simulation Study

PubMed Central

2015-01-01

Dual energy methods can suppress the contrast between adipose and glandular tissues in the breast and therefore enhance the visibility of calcifications. In this study, a dual energy method based on analytical modeling was developed for the detection of minimum microcalcification thickness. To this aim, a modified radiographic X-ray unit was considered, in order to overcome the limited kVp range of mammographic units used in previous DE studies, combined with a high resolution CMOS sensor (pixel size of 22.5 μm) for improved resolution. Various filter materials were examined based on their K-absorption edge. Hydroxyapatite (HAp) was used to simulate microcalcifications. The contrast to noise ratio (CNRtc) of the subtracted images was calculated for both monoenergetic and polyenergetic X-ray beams. The optimum monoenergetic pair was 23/58 keV for the low and high energy, respectively, resulting in a minimum detectable microcalcification thickness of 100 μm. In the polyenergetic X-ray study, the optimal spectral combination was 40/70 kVp filtered with 100 μm cadmium and 1000 μm copper, respectively. In this case, the minimum detectable microcalcification thickness was 150 μm. The proposed dual energy method provides improved microcalcification detectability in breast imaging with mean glandular dose values within acceptable levels. PMID:26246848

Computation of distribution of minimum resolution for log-normal distribution of chromatographic peak heights.

PubMed

Davis, Joe M

2011-10-28

General equations are derived for the distribution of minimum resolution between two chromatographic peaks, when peak heights in a multi-component chromatogram follow a continuous statistical distribution. The derivation draws on published theory by relating the area under the distribution of minimum resolution to the area under the distribution of the ratio of peak heights, which in turn is derived from the peak-height distribution. Two procedures are proposed for the equations' numerical solution. The procedures are applied to the log-normal distribution, which recently was reported to describe the distribution of component concentrations in three complex natural mixtures. For published statistical parameters of these mixtures, the distribution of minimum resolution is similar to that for the commonly assumed exponential distribution of peak heights used in statistical-overlap theory. However, these two distributions of minimum resolution can differ markedly, depending on the scale parameter of the log-normal distribution. Theory for the computation of the distribution of minimum resolution is extended to other cases of interest. With the log-normal distribution of peak heights as an example, the distribution of minimum resolution is computed when small peaks are lost due to noise or detection limits, and when the height of at least one peak is less than an upper limit. The distribution of minimum resolution shifts slightly to lower resolution values in the first case and to markedly larger resolution values in the second one. The theory and numerical procedure are confirmed by Monte Carlo simulation. Copyright © 2011 Elsevier B.V. All rights reserved.

Assessment of Hydrogen Sulfide Minimum Detection Limits of an Open Path Tunable Diode Laser

EPA Science Inventory

During June 2007, U.S. EPA conducted a feasibility study to determine whether the EPA OTM 10 measurement approach, also known as radial plume mapping (RPM), was feasible. A Boreal open-path tunable diode laser (OP-TDL) to collect path-integrated hydrogen sulfide measurements alon...

Ammonia Optical Sensing by Microring Resonators

PubMed Central

Passaro, Vittorio M. N.; Dell'Olio, Francesco; De Leonardis, Francesco

2007-01-01

A very compact (device area around 40 μm2) optical ammonia sensor based on a microring resonator is presented in this work. Silicon-on-insulator technology is used in sensor design and a dye doped polymer is adopted as sensing material. The sensor exhibits a very good linearity and a minimum detectable refractive index shift of sensing material as low as 8×10-5, with a detection limit around 4 ‰. PMID:28903258

Validation of Passive Sampling Devices for Monitoring of Munitions Constituents in Underwater Environments

DTIC Science & Technology

2017-09-01

this project, we launched at Esperanza pier (Figure 5-4), which required a minimum of 2 hours of travel time , including transit from Camp Garcia to the...concentrations of emerging contaminants by providing a time -integrated sample with low detection limits and in situ extraction. PSDs are fairly well...A continuous sampling approach allows detection and quantification of chemicals in an integrated manner, providing time - weighted average (TWA

HST images of the eclipsing pulsar B1957+20

NASA Technical Reports Server (NTRS)

Fruchter, Andrew S.; Bookbinder, Jay; Bailyn, Charles D.

1995-01-01

We have obtained images of the eclipsing pulsar binary PSR B1957+20 using the Planetary Camera of the Hubble Space Telescope (HST). The high spatial resolution of this instrument has allowed us to separate the pulsar system from a nearby background star which has confounded ground-based observations of this system near optical minimum. Our images limit the temperature of the backside of the companion to T less than or approximately = 2800 K, about a factor of 2 less than the average temperature of the side of the companion facing the pulsar, and provide a marginal detection of the companion at optical minimum. The magnitude of this detection is consistent with previous work which suggests that the companion nearly fills its Roche lobe and is supported through tidal dissipation.

Natural gas pipeline leak detector based on NIR diode laser absorption spectroscopy.

PubMed

Gao, Xiaoming; Fan, Hong; Huang, Teng; Wang, Xia; Bao, Jian; Li, Xiaoyun; Huang, Wei; Zhang, Weijun

2006-09-01

The paper reports on the development of an integrated natural gas pipeline leak detector based on diode laser absorption spectroscopy. The detector transmits a 1.653 microm DFB diode laser with 10 mW and detects a fraction of the backscatter reflected from the topographic targets. To eliminate the effect of topographic scatter targets, a ratio detection technique was used. Wavelength modulation and harmonic detection were used to improve the detection sensitivity. The experimental detection limit is 50 ppmm, remote detection for a distance up to 20 m away topographic scatter target is demonstrated. Using a known simulative leak pipe, minimum detectable pipe leak flux is less than 10 ml/min.

A fluorescent immunochromatographic strip test using a quantum dot-antibody probe for rapid and quantitative detection of 1-aminohydantoin in edible animal tissues.

PubMed

Le, Tao; Zhang, Zhihao; Wu, Juan; Shi, Haixing; Cao, Xudong

2018-01-01

A rapid, simple, and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) has been developed to detect 1-aminohydantoin (AHD), a major metabolite of nitrofurantoin in animal tissues. To achieve this, QD-labeled antibody conjugates, which consist of CdSe/ZnS QDs and monoclonal antibodies, were prepared by an activated ester method. Under optimal conditions, with the nitrophenyl derivative of AHD as the target, the ICST had a linear range from 0.1 to 100 ng/mL, with a correlation coefficient of 0.9656 and a 50% inhibitory concentration of 4.51 ng/mL. The limit of detection was 0.14 ng/g, which was below the minimum required performance limit of 1 μg/kg for AHD established by the European Commission. The recoveries for AHD ranged from 81.5% to 108.2%, with coefficients of variation below 13%, based on intraday and interday analysis. Furthermore, for AHD in real samples, the ICST showed high reliability and high correlation with liquid chromatography-tandem mass spectrometry (correlation coefficient of 0.9916). To the best of our knowledge, this is the first report of a novel and sensitive method based on a fluorescent ICST to detect AHD below the minimum required performance limit. The ICST demonstrated high reliability, and could be ideally suited for rapid, simple, and on-site screening of AHD contamination in animal tissues. Graphical abstract A rapid, simple, and sensitive fluorescent immunochromatographic strip test that is based on quantum dots was developed to detect 1-aminohydantoin (AHD), a major metabolite of nitrofurantoin in animal tissues. 2-NBA 2-nitrobenzaldehyde, NP nitrophenyl.

Compact TDLAS based sensor design using interband cascade lasers for mid-IR trace gas sensing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dong, Lei; Tittel, Frank K.; Li, Chunguang

2016-02-25

Two compact TDLAS sensor systems based on different structural optical cores were developed. The two optical cores combine two recent developments, gallium antimonide (GaSb)-based ICL and a compact multipass gas cell (MPGC) with the goal to create compact TDLAS based sensors for the mid-IR gas detection with high detection sensitivity and low power consumption. The sensors achieved minimum detection limits of ~5 ppbv and ~8 ppbv, respectively, for CH 4 and C 2H 6 concentration measurements with a 3.7-W power consumption.

Novel utilisation of a circular multi-reflection cell applied to materials ageing experiments

NASA Astrophysics Data System (ADS)

Knox, D. A.; King, A. K.; McNaghten, E. D.; Brooks, S. J.; Martin, P. A.; Pimblott, S. M.

2015-04-01

We report on the novel utilisation of a circular multi-reflection (CMR) cell applied to materials ageing experiments. This enabled trace gas detection within a narrow interfacial region located between two sample materials and remotely interrogated with near-infrared sources combined with fibre-optic coupling. Tunable diode laser absorption spectroscopy was used to detect water vapour and carbon dioxide at wavelengths near 1,358 and 2,004 nm, respectively, with corresponding detection limits of 7 and 1,139 ppm m Hz-0.5. The minimum detectable absorption was estimated to be 2.82 × 10-3 over a 1-s average. In addition, broadband absorption spectroscopy was carried out for the detection of acetic acid, using a super-luminescent light emitting diode centred around 1,430 nm. The 69 cm measurement pathlength was limited by poor manufacturing tolerances of the spherical CMR mirrors and the consequent difficulty of collecting all the cell output light.

Capacity of optical communications over a lossy bosonic channel with a receiver employing the most general coherent electro-optic feedback control

NASA Astrophysics Data System (ADS)

Chung, Hye Won; Guha, Saikat; Zheng, Lizhong

2017-07-01

We study the problem of designing optical receivers to discriminate between multiple coherent states using coherent processing receivers—i.e., one that uses arbitrary coherent feedback control and quantum-noise-limited direct detection—which was shown by Dolinar to achieve the minimum error probability in discriminating any two coherent states. We first derive and reinterpret Dolinar's binary-hypothesis minimum-probability-of-error receiver as the one that optimizes the information efficiency at each time instant, based on recursive Bayesian updates within the receiver. Using this viewpoint, we propose a natural generalization of Dolinar's receiver design to discriminate M coherent states, each of which could now be a codeword, i.e., a sequence of N coherent states, each drawn from a modulation alphabet. We analyze the channel capacity of the pure-loss optical channel with a general coherent-processing receiver in the low-photon number regime and compare it with the capacity achievable with direct detection and the Holevo limit (achieving the latter would require a quantum joint-detection receiver). We show compelling evidence that despite the optimal performance of Dolinar's receiver for the binary coherent-state hypothesis test (either in error probability or mutual information), the asymptotic communication rate achievable by such a coherent-processing receiver is only as good as direct detection. This suggests that in the infinitely long codeword limit, all potential benefits of coherent processing at the receiver can be obtained by designing a good code and direct detection, with no feedback within the receiver.

Demonstration and Validation of a Regenerated Cellulose Dialysis Membrane Diffusion Sampler for Monitoring Ground Water Quality and Remediation Progress at DoD Sites

DTIC Science & Technology

2007-08-30

ITRC Interstate Technology Regulatory Council LRL Laboratory reporting level LDPE Low-density polyethylene MDL Minimum detection limit MNA...diameter of the well. Another diffusion membrane sampler design consists of a tubular-shaped bag made of flexible low-density polyethylene ( LDPE ...

fM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor

PubMed Central

Huang, Guoliang; Huang, Qin; Ma, Li; Luo, Xianbo; Pang, Biao; Zhang, Zhixin; Wang, Ruliang; Zhang, Junqi; Li, Qi; Fu, Rongxin; Ye, Jiancheng

2014-01-01

A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.3 aM, and a detection limit of three copies of genomic DNA was obtained. This microfluidic bioreactor offers a promising clinically relevant pathogen molecular diagnostic method via the amplification of targets from only a few copies of genomic DNA from a single bacterium. PMID:25475544

Calculation of the detection limit in radiation measurements with systematic uncertainties

NASA Astrophysics Data System (ADS)

Kirkpatrick, J. M.; Russ, W.; Venkataraman, R.; Young, B. M.

2015-06-01

The detection limit (LD) or Minimum Detectable Activity (MDA) is an a priori evaluation of assay sensitivity intended to quantify the suitability of an instrument or measurement arrangement for the needs of a given application. Traditional approaches as pioneered by Currie rely on Gaussian approximations to yield simple, closed-form solutions, and neglect the effects of systematic uncertainties in the instrument calibration. These approximations are applicable over a wide range of applications, but are of limited use in low-count applications, when high confidence values are required, or when systematic uncertainties are significant. One proposed modification to the Currie formulation attempts account for systematic uncertainties within a Gaussian framework. We have previously shown that this approach results in an approximation formula that works best only for small values of the relative systematic uncertainty, for which the modification of Currie's method is the least necessary, and that it significantly overestimates the detection limit or gives infinite or otherwise non-physical results for larger systematic uncertainties where such a correction would be the most useful. We have developed an alternative approach for calculating detection limits based on realistic statistical modeling of the counting distributions which accurately represents statistical and systematic uncertainties. Instead of a closed form solution, numerical and iterative methods are used to evaluate the result. Accurate detection limits can be obtained by this method for the general case.

Quantum cascade laser-based multipass absorption system for hydrogen peroxide detection

NASA Astrophysics Data System (ADS)

Cao, Yingchun; Sanchez, Nancy P.; Jiang, Wenzhe; Ren, Wei; Lewicki, Rafal; Jiang, Dongfang; Griffin, Robert J.; Tittel, Frank K.

2015-01-01

Hydrogen peroxide (H2O2) is a relevant molecular trace gas species, that is related to the oxidative capacity of the atmosphere, the production of radical species such as OH, the generation of sulfate aerosol via oxidation of S(IV) to S(VI), and the formation of acid rain. The detection of atmospheric H2O2 involves specific challenges due to its high reactivity and low concentration (ppbv to sub-ppbv level). Traditional methods for measuring atmospheric H2O2 concentration are often based on wet-chemistry methods that require a transfer from the gas- to liquid-phase for a subsequent determination by techniques such as fluorescence spectroscopy, which can lead to problems such as sampling artifacts and interference by other atmospheric constituents. A quartz-enhanced photoacoustic spectroscopy-based system for the measurement of atmospheric H2O2 with a detection limit of 75 ppb for 1-s integration time was previously reported. In this paper, an updated H2O2 detection system based on long-optical-path-length absorption spectroscopy by using a distributed feedback quantum cascade laser (DFB-QCL) will be described. A 7.73-μm CW-DFB-QCL and a thermoelectrically cooled infrared detector, optimized for a wavelength of 8 μm, are employed for theH2O2 sensor system. A commercial astigmatic Herriott multi-pass cell with an effective optical path-length of 76 m is utilized for the reported QCL multipass absorption system. Wavelength modulation spectroscopy (WMS) with second harmonic detection is used for enhancing the signal-to-noise-ratio. A minimum detection limit of 13.4 ppb is achieved with a 2 s sampling time. Based on an Allan-Werle deviation analysis the minimum detection limit can be improved to 1.5 ppb when using an averaging time of 300 s.

Methods for the preparation and analysis of solids and suspended solids for total mercury

USGS Publications Warehouse

Olund, Shane D.; DeWild, John F.; Olson, Mark L.; Tate, Michael T.

2004-01-01

The methods documented in this report are utilized by the Wisconsin District Mercury Lab for analysis of total mercury in solids (soils and sediments) and suspended solids (isolated on filters). Separate procedures are required for the different sample types. For solids, samples are prepared by room-temperature acid digestion and oxidation with aqua regia. The samples are brought up to volume with a 5 percent bromine monochloride solution to ensure complete oxidation and heated at 50?C in an oven overnight. Samples are then analyzed with an automated flow injection system incorporating a cold vapor atomic fluorescence spectrometer. A method detection limit of 0.3 ng of mercury per digestion bomb was established using multiple analyses of an environmental sample. Based on the range of masses processed, the minimum sample reporting limit varies from 0.6 ng/g to 6 ng/g. Suspended solids samples are oxidized with a 5 percent bromine monochloride solution and held at 50?C in an oven for 5 days. The samples are then analyzed with an automated flow injection system incorporating a cold vapor atomic fluorescence spectrometer. Using a certified reference material as a surrogate for an environmental sample, a method detection limit of 0.059 ng of mercury per filter was established. The minimum sample reporting limit varies from 0.059 ng/L to 1.18 ng/L, depending on the volume of water filtered.

Development of Detectability Limits for On-Orbit Inspection of Space Shuttle Wing Leading Edge

NASA Technical Reports Server (NTRS)

Stephan, Ryan A.; Johnson, David G.; Mastropietro, A. J.; Ancarrow, Walt C.

2005-01-01

At the conclusion of the Columbia Accident Investigation, one of the recommendations of the Columbia Accident Investigation Board (CAIB) was that NASA develop and implement an inspection plan for the Reinforced Carbon-Carbon (RCC) system components of the Space Shuttle. To address these issues, a group of scientists and engineers at NASA Langley Research Center proposed the use of an IR camera to inspect the RCC. Any crack in an RCC panel changes the thermal resistance of the material in the direction perpendicular to the crack. The change in thermal resistance can be made visible by introducing a heat flow across the crack and using an IR camera to image the resulting surface temperature distribution. The temperature difference across the crack depends on the change in the thermal resistance, the length of the crack, the local thermal gradient, and the rate of radiation exchange with the environment. This paper describes how the authors derived the minimum thermal gradient detectability limits for a through crack in an RCC panel. This paper will also show, through the use of a transient, 3-dimensional, finite element model, that these minimum gradients naturally exist on-orbit. The results from the finite element model confirm that there are sufficient thermal gradient to detect a crack on 96% of the RCC leading edge.

High-field modulated ion-selective field-effect-transistor (FET) sensors with sensitivity higher than the ideal Nernst sensitivity.

PubMed

Chen, Yi-Ting; Sarangadharan, Indu; Sukesan, Revathi; Hseih, Ching-Yen; Lee, Geng-Yen; Chyi, Jen-Inn; Wang, Yu-Lin

2018-05-29

Lead ion selective membrane (Pb-ISM) coated AlGaN/GaN high electron mobility transistors (HEMT) was used to demonstrate a whole new methodology for ion-selective FET sensors, which can create ultra-high sensitivity (-36 mV/log [Pb 2+ ]) surpassing the limit of ideal sensitivity (-29.58 mV/log [Pb 2+ ]) in a typical Nernst equation for lead ion. The largely improved sensitivity has tremendously reduced the detection limit (10 -10  M) for several orders of magnitude of lead ion concentration compared to typical ion-selective electrode (ISE) (10 -7  M). The high sensitivity was obtained by creating a strong filed between the gate electrode and the HEMT channel. Systematical investigation was done by measuring different design of the sensor and gate bias, indicating ultra-high sensitivity and ultra-low detection limit obtained only in sufficiently strong field. Theoretical study in the sensitivity consistently agrees with the experimental finding and predicts the maximum and minimum sensitivity. The detection limit of our sensor is comparable to that of Inductively-Coupled-Plasma Mass Spectrum (ICP-MS), which also has detection limit near 10 -10  M.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Wenfang; Du, Jinjin; Wen, Ruijuan

We have investigated the transmission spectra of a Fabry-Perot interferometer (FPI) with squeezed vacuum state injection and non-Gaussian detection, including photon number resolving detection and parity detection. In order to show the suitability of the system, parallel studies were made of the performance of two other light sources: coherent state of light and Fock state of light either with classical mean intensity detection or with non-Gaussian detection. This shows that by using the squeezed vacuum state and non-Gaussian detection simultaneously, the resolution of the FPI can go far beyond the cavity standard bandwidth limit based on the current techniques. Themore » sensitivity of the scheme has also been explored and it shows that the minimum detectable sensitivity is better than that of the other schemes.« less

Verification of Minimum Detectable Activity for Radiological Threat Source Search

NASA Astrophysics Data System (ADS)

Gardiner, Hannah; Myjak, Mitchell; Baciak, James; Detwiler, Rebecca; Seifert, Carolyn

2015-10-01

The Department of Homeland Security's Domestic Nuclear Detection Office is working to develop advanced technologies that will improve the ability to detect, localize, and identify radiological and nuclear sources from airborne platforms. The Airborne Radiological Enhanced-sensor System (ARES) program is developing advanced data fusion algorithms for analyzing data from a helicopter-mounted radiation detector. This detector platform provides a rapid, wide-area assessment of radiological conditions at ground level. The NSCRAD (Nuisance-rejection Spectral Comparison Ratios for Anomaly Detection) algorithm was developed to distinguish low-count sources of interest from benign naturally occurring radiation and irrelevant nuisance sources. It uses a number of broad, overlapping regions of interest to statistically compare each newly measured spectrum with the current estimate for the background to identify anomalies. We recently developed a method to estimate the minimum detectable activity (MDA) of NSCRAD in real time. We present this method here and report on the MDA verification using both laboratory measurements and simulated injects on measured backgrounds at or near the detection limits. This work is supported by the US Department of Homeland Security, Domestic Nuclear Detection Office, under competitively awarded contract/IAA HSHQDC-12-X-00376. This support does not constitute an express or implied endorsement on the part of the Gov't.

Electronic Nose Testing Procedure for the Definition of Minimum Performance Requirements for Environmental Odor Monitoring

PubMed Central

Eusebio, Lidia; Capelli, Laura; Sironi, Selena

2016-01-01

Despite initial enthusiasm towards electronic noses and their possible application in different fields, and quite a lot of promising results, several criticalities emerge from most published research studies, and, as a matter of fact, the diffusion of electronic noses in real-life applications is still very limited. In general, a first step towards large-scale-diffusion of an analysis method, is standardization. The aim of this paper is describing the experimental procedure adopted in order to evaluate electronic nose performances, with the final purpose of establishing minimum performance requirements, which is considered to be a first crucial step towards standardization of the specific case of electronic nose application for environmental odor monitoring at receptors. Based on the experimental results of the performance testing of a commercialized electronic nose type with respect to three criteria (i.e., response invariability to variable atmospheric conditions, instrumental detection limit, and odor classification accuracy), it was possible to hypothesize a logic that could be adopted for the definition of minimum performance requirements, according to the idea that these are technologically achievable. PMID:27657086

Electronic Nose Testing Procedure for the Definition of Minimum Performance Requirements for Environmental Odor Monitoring.

PubMed

Eusebio, Lidia; Capelli, Laura; Sironi, Selena

2016-09-21

Despite initial enthusiasm towards electronic noses and their possible application in different fields, and quite a lot of promising results, several criticalities emerge from most published research studies, and, as a matter of fact, the diffusion of electronic noses in real-life applications is still very limited. In general, a first step towards large-scale-diffusion of an analysis method, is standardization. The aim of this paper is describing the experimental procedure adopted in order to evaluate electronic nose performances, with the final purpose of establishing minimum performance requirements, which is considered to be a first crucial step towards standardization of the specific case of electronic nose application for environmental odor monitoring at receptors. Based on the experimental results of the performance testing of a commercialized electronic nose type with respect to three criteria (i.e., response invariability to variable atmospheric conditions, instrumental detection limit, and odor classification accuracy), it was possible to hypothesize a logic that could be adopted for the definition of minimum performance requirements, according to the idea that these are technologically achievable.

A green, reusable SERS film with high sensitivity for in-situ detection of thiram in apple juice

NASA Astrophysics Data System (ADS)

Sun, Hongbao; Liu, Hai; Wu, Yiyong

2017-09-01

We report a green and reusable surface-enhanced Raman scattering (SERS) film based on PMMA/Ag NPs/graphene. By using this Raman substrate, the SERS signals of R6G were significantly enhanced reaching a minimum detectable concentration of 5 × 10-8 M, due to having lots of hot spots adhered backside to the exposed graphene. The SERS film can be used for in-situ monitoring of trace thiram in apple juice with a detection limit of 1 × 10-6 M (0.24 ppm), which is below the maximal residue limit (MRL) of 7 ppm in fruit prescribed by the U.S. Environmental Protection Agency (EPA). Furthermore, reusability studies show that the SERS film can be used repeatedly. In addition, the graphene-enhanced SERS technique shows great potential applications for the in-situ detection and identification of pesticide residues in environmental water, fruits and vegetables.

A practical approach to determination of laboratory GC-MS limits of detection.

PubMed

Underwood, P J; Kananen, G E; Armitage, E K

1997-01-01

Determination of limit of detection (LOD) values in a forensic laboratory serves a fundamental forensic requirement for assay performance. In addition to demonstrating assay capability, LOD values can also be used to fulfill certification requirements of a high-volume forensic drug laboratory. The LOD was defined as the lowest concentration of drug that the laboratory can detect in a specimen with forensic certainty at a minimum of 85% of the time. Overall batch acceptance criteria included acceptable quantitation of control materials (within 20% of target), acceptable chromatography (symmetry, peak integration, peak shape, peak, and baseline resolution), retention time within +/-1% of the extracted standard, and mass ion ratios within +/-20% of the extracted standard mass ion ratios. Individual specimen acceptance criteria were the same as the batch acceptance criteria excluding the quantitation requirement. Data were collected from all instruments on different runs. A minimum of ten data points was required for each certified instrument, and a minimum of 85% of data points was acceptable. Quantitation within +/-20% of the LOD concentration was not required, but acceptable mass ratios were required. Data points with poor chromatography (internal standard failed mass ratios; interference of the baseline, for example, shoulders; asymmetry; and baseline resolution) was omitted from the acceptable rate calculation. Data points with good chromatography with failed mass ion ratios were included in the acceptable rate calculation. With these criteria, we established the following LODs: 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid, 2 ng/mL; benzoylecgonine, 5 ng/mL; phencyclidine, 2.5 ng/mL; amphetamine, 150 ng/mL; methamphetamine, 100 ng/mL; codeine, 500 ng/mL; and morphine, 1000 ng/mL.

Multipoint Optimal Minimum Entropy Deconvolution and Convolution Fix: Application to vibration fault detection

NASA Astrophysics Data System (ADS)

McDonald, Geoff L.; Zhao, Qing

2017-01-01

Minimum Entropy Deconvolution (MED) has been applied successfully to rotating machine fault detection from vibration data, however this method has limitations. A convolution adjustment to the MED definition and solution is proposed in this paper to address the discontinuity at the start of the signal - in some cases causing spurious impulses to be erroneously deconvolved. A problem with the MED solution is that it is an iterative selection process, and will not necessarily design an optimal filter for the posed problem. Additionally, the problem goal in MED prefers to deconvolve a single-impulse, while in rotating machine faults we expect one impulse-like vibration source per rotational period of the faulty element. Maximum Correlated Kurtosis Deconvolution was proposed to address some of these problems, and although it solves the target goal of multiple periodic impulses, it is still an iterative non-optimal solution to the posed problem and only solves for a limited set of impulses in a row. Ideally, the problem goal should target an impulse train as the output goal, and should directly solve for the optimal filter in a non-iterative manner. To meet these goals, we propose a non-iterative deconvolution approach called Multipoint Optimal Minimum Entropy Deconvolution Adjusted (MOMEDA). MOMEDA proposes a deconvolution problem with an infinite impulse train as the goal and the optimal filter solution can be solved for directly. From experimental data on a gearbox with and without a gear tooth chip, we show that MOMEDA and its deconvolution spectrums according to the period between the impulses can be used to detect faults and study the health of rotating machine elements effectively.

Plasmonic trace sensing below the photon shot noise limit

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pooser, Raphael C.; Lawrie, Benjamin J.

Plasmonic sensors are important detectors of biochemical trace compounds, but those that utilize optical readout are approaching their absolute limits of detection as defined by the Heisenberg uncertainty principle in both differential intensity and phase readout. However, the use of more general minimum uncertainty states in the form of squeezed light can push the noise floor in these sensors below the shot noise limit (SNL) in one analysis variable at the expense of another. Here, we demonstrate a quantum plasmonic sensor whose noise floor is reduced below the SNL in order to perform index of refraction measurements with sensitivities unobtainablemore » with classical plasmonic sensors. The increased signal-to-noise ratio can result in faster detection of analyte concentrations that were previously lost in the noise. As a result, these benefits are the hallmarks of a sensor exploiting quantum readout fields in order to manipulate the limits of the Heisenberg uncertainty principle.« less

Plasmonic trace sensing below the photon shot noise limit

DOE PAGES

Pooser, Raphael C.; Lawrie, Benjamin J.

2015-12-09

Plasmonic sensors are important detectors of biochemical trace compounds, but those that utilize optical readout are approaching their absolute limits of detection as defined by the Heisenberg uncertainty principle in both differential intensity and phase readout. However, the use of more general minimum uncertainty states in the form of squeezed light can push the noise floor in these sensors below the shot noise limit (SNL) in one analysis variable at the expense of another. Here, we demonstrate a quantum plasmonic sensor whose noise floor is reduced below the SNL in order to perform index of refraction measurements with sensitivities unobtainablemore » with classical plasmonic sensors. The increased signal-to-noise ratio can result in faster detection of analyte concentrations that were previously lost in the noise. As a result, these benefits are the hallmarks of a sensor exploiting quantum readout fields in order to manipulate the limits of the Heisenberg uncertainty principle.« less

"PowerUp"!: A Tool for Calculating Minimum Detectable Effect Sizes and Minimum Required Sample Sizes for Experimental and Quasi-Experimental Design Studies

ERIC Educational Resources Information Center

Dong, Nianbo; Maynard, Rebecca

2013-01-01

This paper and the accompanying tool are intended to complement existing supports for conducting power analysis tools by offering a tool based on the framework of Minimum Detectable Effect Sizes (MDES) formulae that can be used in determining sample size requirements and in estimating minimum detectable effect sizes for a range of individual- and…

Low volume flow meter

DOEpatents

Meixler, Lewis D.

1993-01-01

The low flow monitor provides a means for determining if a fluid flow meets a minimum threshold level of flow. The low flow monitor operates with a minimum of intrusion by the flow detection device into the flow. The electrical portion of the monitor is externally located with respect to the fluid stream which allows for repairs to the monitor without disrupting the flow. The electronics provide for the adjustment of the threshold level to meet the required conditions. The apparatus can be modified to provide an upper limit to the flow monitor by providing for a parallel electronic circuit which provides for a bracketing of the desired flow rate.

Cs2AgBiBr6 single-crystal X-ray detectors with a low detection limit

NASA Astrophysics Data System (ADS)

Pan, Weicheng; Wu, Haodi; Luo, Jiajun; Deng, Zhenzhou; Ge, Cong; Chen, Chao; Jiang, Xiaowei; Yin, Wan-Jian; Niu, Guangda; Zhu, Lujun; Yin, Lixiao; Zhou, Ying; Xie, Qingguo; Ke, Xiaoxing; Sui, Manling; Tang, Jiang

2017-11-01

Sensitive X-ray detection is crucial for medical diagnosis, industrial inspection and scientific research. The recently described hybrid lead halide perovskites have demonstrated low-cost fabrication and outstanding performance for direct X-ray detection, but they all contain toxic Pb in a soluble form. Here, we report sensitive X-ray detectors using solution-processed double perovskite Cs2AgBiBr6 single crystals. Through thermal annealing and surface treatment, we largely eliminate Ag+/Bi3+ disordering and improve the crystal resistivity, resulting in a detector with a minimum detectable dose rate as low as 59.7 nGyair s-1, comparable to the latest record of 0.036 μGyair s-1 using CH3NH3PbBr3 single crystals. Suppressed ion migration in Cs2AgBiBr6 permits relatively large external bias, guaranteeing efficient charge collection without a substantial increase in noise current and thus enabling the low detection limit.

Minimum detectable gas concentration performance evaluation method for gas leak infrared imaging detection systems.

PubMed

Zhang, Xu; Jin, Weiqi; Li, Jiakun; Wang, Xia; Li, Shuo

2017-04-01

Thermal imaging technology is an effective means of detecting hazardous gas leaks. Much attention has been paid to evaluation of the performance of gas leak infrared imaging detection systems due to several potential applications. The minimum resolvable temperature difference (MRTD) and the minimum detectable temperature difference (MDTD) are commonly used as the main indicators of thermal imaging system performance. This paper establishes a minimum detectable gas concentration (MDGC) performance evaluation model based on the definition and derivation of MDTD. We proposed the direct calculation and equivalent calculation method of MDGC based on the MDTD measurement system. We build an experimental MDGC measurement system, which indicates the MDGC model can describe the detection performance of a thermal imaging system to typical gases. The direct calculation, equivalent calculation, and direct measurement results are consistent. The MDGC and the minimum resolvable gas concentration (MRGC) model can effectively describe the performance of "detection" and "spatial detail resolution" of thermal imaging systems to gas leak, respectively, and constitute the main performance indicators of gas leak detection systems.

Soil C and N minimum detectable changes and treatment differences in a multi-treatment forest experiment.

Treesearch

P.S. Homann; B.T. Bormann; J.R. Boyle; R.L. Darbyshire; R. Bigley

2008-01-01

Detecting changes in forest soil C and N is vital to the study of global budgets and long-term ecosystem productivity. Identifying differences among land-use practices may guide future management. Our objective was to determine the relation of minimum detectable changes (MDCs) and minimum detectable differences between treatments (MDDs) to soil C and N variability at...

Extension of Gutenberg-Richter distribution to MW -1.3, no lower limit in sight

NASA Astrophysics Data System (ADS)

Boettcher, Margaret S.; McGarr, A.; Johnston, Malcolm

2009-05-01

With twelve years of seismic data from TauTona Gold Mine, South Africa, we show that mining-induced earthquakes follow the Gutenberg-Richter relation with no scale break down to the completeness level of the catalog, at moment magnitude M W -1.3. Events recorded during relatively quiet hours in 2006 indicate that catalog detection limitations, not earthquake source physics, controlled the previously reported minimum magnitude in this mine. Within the Natural Earthquake Laboratory in South African Mines (NELSAM) experiment's dense seismic array, earthquakes that exhibit shear failure at magnitudes as small as M W -3.9 are observed, but we find no evidence that M W -3.9 represents the minimum magnitude. In contrast to previous work, our results imply small nucleation zones and that earthquake processes in the mine can readily be scaled to those in either laboratory experiments or natural faults.

Extension of Gutenberg-Richter distribution to Mw -1.3, no lower limit in sight

USGS Publications Warehouse

Boettcher, M.S.; McGarr, A.; Johnston, M.

2009-01-01

[1] With twelve years of seismic data from TauTona Gold Mine, South Africa, we show that mining-induced earthquakes follow the Gutenberg-Richter relation with no scale break down to the completeness level of the catalog, at moment magnitude Mw -1.3. Events recorded during relatively quiet hours in 2006 indicate that catalog detection limitations, not earthquake source physics, controlled the previously reported minimum magnitude in this mine. Within the Natural Earthquake Laboratory in South African Mines (NELSAM) experiment's dense seismic array, earthquakes that exhibit shear failure at magnitudes as small as Mw -3.9 are observed, but we find no evidence that Mw -3.9 represents the minimum magnitude. In contrast to previous work, our results imply small nucleation zones and that earthquake processes in the mine can readily be scaled to those in either laboratory experiments or natural faults.

An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites.

PubMed

Butterworth, Alice S; Robertson, Alan J; Ho, Mei-Fong; Gatton, Michelle L; McCarthy, James S; Trenholme, Katharine R

2011-04-18

Obtaining single parasite clones is required for many techniques in malaria research. Cloning by limiting dilution using microscopy-based assessment for parasite growth is an arduous and labor-intensive process. An alternative method for the detection of parasite growth in limiting dilution assays is using a commercial ELISA histidine-rich protein II (HRP2) detection kit. Detection of parasite growth was undertaken using HRP2 ELISA and compared to thick film microscopy. An HRP2 protein standard was used to determine the detection threshold of the HRP2 ELISA assay, and a HRP2 release model was used to extrapolate the amount of parasite growth required for a positive result. The HRP2 ELISA was more sensitive than microscopy for detecting parasite growth. The minimum level of HRP2 protein detection of the ELISA was 0.11 ng/ml. Modeling of HRP2 release determined that 2,116 parasites are required to complete a full erythrocytic cycle to produce sufficient HRP2 to be detected by the ELISA. Under standard culture conditions this number of parasites is likely to be reached between 8 to 14 days of culture. This method provides an accurate and simple way for the detection of parasite growth in limiting dilution assays, reducing time and resources required in traditional methods. Furthermore the method uses spent culture media instead of the parasite-infected red blood cells, enabling culture to continue. © 2011 Butterworth et al; licensee BioMed Central Ltd.

Theoretical detection threshold of the proton-acoustic range verification technique.

PubMed

Ahmad, Moiz; Xiang, Liangzhong; Yousefi, Siavash; Xing, Lei

2015-10-01

Range verification in proton therapy using the proton-acoustic signal induced in the Bragg peak was investigated for typical clinical scenarios. The signal generation and detection processes were simulated in order to determine the signal-to-noise limits. An analytical model was used to calculate the dose distribution and local pressure rise (per proton) for beams of different energy (100 and 160 MeV) and spot widths (1, 5, and 10 mm) in a water phantom. In this method, the acoustic waves propagating from the Bragg peak were generated by the general 3D pressure wave equation implemented using a finite element method. Various beam pulse widths (0.1-10 μs) were simulated by convolving the acoustic waves with Gaussian kernels. A realistic PZT ultrasound transducer (5 cm diameter) was simulated with a Butterworth bandpass filter with consideration of random noise based on a model of thermal noise in the transducer. The signal-to-noise ratio on a per-proton basis was calculated, determining the minimum number of protons required to generate a detectable pulse. The maximum spatial resolution of the proton-acoustic imaging modality was also estimated from the signal spectrum. The calculated noise in the transducer was 12-28 mPa, depending on the transducer central frequency (70-380 kHz). The minimum number of protons detectable by the technique was on the order of 3-30 × 10(6) per pulse, with 30-800 mGy dose per pulse at the Bragg peak. Wider pulses produced signal with lower acoustic frequencies, with 10 μs pulses producing signals with frequency less than 100 kHz. The proton-acoustic process was simulated using a realistic model and the minimal detection limit was established for proton-acoustic range validation. These limits correspond to a best case scenario with a single large detector with no losses and detector thermal noise as the sensitivity limiting factor. Our study indicated practical proton-acoustic range verification may be feasible with approximately 5 × 10(6) protons/pulse and beam current.

Theoretical detection threshold of the proton-acoustic range verification technique

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahmad, Moiz; Yousefi, Siavash; Xing, Lei, E-mail: lei@stanford.edu

2015-10-15

Purpose: Range verification in proton therapy using the proton-acoustic signal induced in the Bragg peak was investigated for typical clinical scenarios. The signal generation and detection processes were simulated in order to determine the signal-to-noise limits. Methods: An analytical model was used to calculate the dose distribution and local pressure rise (per proton) for beams of different energy (100 and 160 MeV) and spot widths (1, 5, and 10 mm) in a water phantom. In this method, the acoustic waves propagating from the Bragg peak were generated by the general 3D pressure wave equation implemented using a finite element method.more » Various beam pulse widths (0.1–10 μs) were simulated by convolving the acoustic waves with Gaussian kernels. A realistic PZT ultrasound transducer (5 cm diameter) was simulated with a Butterworth bandpass filter with consideration of random noise based on a model of thermal noise in the transducer. The signal-to-noise ratio on a per-proton basis was calculated, determining the minimum number of protons required to generate a detectable pulse. The maximum spatial resolution of the proton-acoustic imaging modality was also estimated from the signal spectrum. Results: The calculated noise in the transducer was 12–28 mPa, depending on the transducer central frequency (70–380 kHz). The minimum number of protons detectable by the technique was on the order of 3–30 × 10{sup 6} per pulse, with 30–800 mGy dose per pulse at the Bragg peak. Wider pulses produced signal with lower acoustic frequencies, with 10 μs pulses producing signals with frequency less than 100 kHz. Conclusions: The proton-acoustic process was simulated using a realistic model and the minimal detection limit was established for proton-acoustic range validation. These limits correspond to a best case scenario with a single large detector with no losses and detector thermal noise as the sensitivity limiting factor. Our study indicated practical proton-acoustic range verification may be feasible with approximately 5 × 10{sup 6} protons/pulse and beam current.« less

Theoretical detection threshold of the proton-acoustic range verification technique

PubMed Central

Ahmad, Moiz; Xiang, Liangzhong; Yousefi, Siavash; Xing, Lei

2015-01-01

Purpose: Range verification in proton therapy using the proton-acoustic signal induced in the Bragg peak was investigated for typical clinical scenarios. The signal generation and detection processes were simulated in order to determine the signal-to-noise limits. Methods: An analytical model was used to calculate the dose distribution and local pressure rise (per proton) for beams of different energy (100 and 160 MeV) and spot widths (1, 5, and 10 mm) in a water phantom. In this method, the acoustic waves propagating from the Bragg peak were generated by the general 3D pressure wave equation implemented using a finite element method. Various beam pulse widths (0.1–10 μs) were simulated by convolving the acoustic waves with Gaussian kernels. A realistic PZT ultrasound transducer (5 cm diameter) was simulated with a Butterworth bandpass filter with consideration of random noise based on a model of thermal noise in the transducer. The signal-to-noise ratio on a per-proton basis was calculated, determining the minimum number of protons required to generate a detectable pulse. The maximum spatial resolution of the proton-acoustic imaging modality was also estimated from the signal spectrum. Results: The calculated noise in the transducer was 12–28 mPa, depending on the transducer central frequency (70–380 kHz). The minimum number of protons detectable by the technique was on the order of 3–30 × 106 per pulse, with 30–800 mGy dose per pulse at the Bragg peak. Wider pulses produced signal with lower acoustic frequencies, with 10 μs pulses producing signals with frequency less than 100 kHz. Conclusions: The proton-acoustic process was simulated using a realistic model and the minimal detection limit was established for proton-acoustic range validation. These limits correspond to a best case scenario with a single large detector with no losses and detector thermal noise as the sensitivity limiting factor. Our study indicated practical proton-acoustic range verification may be feasible with approximately 5 × 106 protons/pulse and beam current. PMID:26429247

Sensitive detection of formaldehyde using an interband cascade laser near 3.6 μm

DOE PAGES

Ren, Wei; Luo, Longqiang; Tittel, Frank K.

2015-12-31

Here, we report the development of a formaldehyde (H 2CO) trace gas sensor using a continuous wave (CW), thermoelectrically-cooled (TEC), distributed-feedback interband cascade laser (DFB-ICL) at 3.6 μm. Wavelength modulation spectroscopy was used to detect the second harmonic spectra of a strong H 2CO absorption feature centered at 2778.5 cm -1 (3599 nm) in its ν 1 fundamental vibrational band. A compact and novel multipass cell (7.6-cm physical length and 32-ml sampling volume) was implemented to achieve an effective optical path length of 3.75 m. A minimum detection limit of 6 parts per billion (ppb) at an optimum gas pressuremore » of 200 Torr was achieved with a 1-s data acquisition time. An Allan-Werle deviation analysis was performed to investigate the long-term stability of the sensor system and a 1.5 ppb minimum detectable concentration could be achieved by averaging up to 140 s. Absorption interference eeffects from atmospheric H 2O (2%) and CH 4(5 ppm) were also analyzed in this work and proved to be insignificant for the current sensor configuration.« less

Sensitive detection of formaldehyde using an interband cascade laser near 3.6 μm

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ren, Wei; Luo, Longqiang; Tittel, Frank K.

Here, we report the development of a formaldehyde (H 2CO) trace gas sensor using a continuous wave (CW), thermoelectrically-cooled (TEC), distributed-feedback interband cascade laser (DFB-ICL) at 3.6 μm. Wavelength modulation spectroscopy was used to detect the second harmonic spectra of a strong H 2CO absorption feature centered at 2778.5 cm -1 (3599 nm) in its ν 1 fundamental vibrational band. A compact and novel multipass cell (7.6-cm physical length and 32-ml sampling volume) was implemented to achieve an effective optical path length of 3.75 m. A minimum detection limit of 6 parts per billion (ppb) at an optimum gas pressuremore » of 200 Torr was achieved with a 1-s data acquisition time. An Allan-Werle deviation analysis was performed to investigate the long-term stability of the sensor system and a 1.5 ppb minimum detectable concentration could be achieved by averaging up to 140 s. Absorption interference eeffects from atmospheric H 2O (2%) and CH 4(5 ppm) were also analyzed in this work and proved to be insignificant for the current sensor configuration.« less

Using Satellite Imagery to Assess Large-Scale Habitat Characteristics of Adirondack Park, New York, USA

NASA Astrophysics Data System (ADS)

McClain, Bobbi J.; Porter, William F.

2000-11-01

Satellite imagery is a useful tool for large-scale habitat analysis; however, its limitations need to be tested. We tested these limitations by varying the methods of a habitat evaluation for white-tailed deer ( Odocoileus virginianus) in the Adirondack Park, New York, USA, utilizing harvest data to create and validate the assessment models. We used two classified images, one with a large minimum mapping unit but high accuracy and one with no minimum mapping unit but slightly lower accuracy, to test the sensitivity of the evaluation to these differences. We tested the utility of two methods of assessment, habitat suitability index modeling, and pattern recognition modeling. We varied the scale at which the models were applied by using five separate sizes of analysis windows. Results showed that the presence of a large minimum mapping unit eliminates important details of the habitat. Window size is relatively unimportant if the data are averaged to a large resolution (i.e., township), but if the data are used at the smaller resolution, then the window size is an important consideration. In the Adirondacks, the proportion of hardwood and softwood in an area is most important to the spatial dynamics of deer populations. The low occurrence of open area in all parts of the park either limits the effect of this cover type on the population or limits our ability to detect the effect. The arrangement and interspersion of cover types were not significant to deer populations.

Reconnaissance investigations of potential ground-water and sediment contamination at three former underground storage tank locations, Fort Jackson, South Carolina, 1994

USGS Publications Warehouse

Robertson, J.F.; Nagle, Douglas D.; Rhodes, Liesl C.

1994-01-01

Investigations to provide initial qualitative delineation of petroleum hydrocarbon contamination at three former underground storage tank locations at Fort Jackson, South Carolina, were made during March 1994. Ground-water and sediment samples were collected using direct-push technology and analyzed on-site with a gas chromatograph, which provided real-time, semi-quantitative data. In addition, ground-water and sediment samples were collected at selected sites for laboratory analyses to provide a confirmation of the on-site data. These analyses provided qualitative data on the lateral distri- bution of petroleum hydrocarbons. Petroleum hydrocarbons were detected by on-site analysis in ground-water samples from nine locations at Site 1062, suggesting the presence of a contaminant plume. Concentrations ranged from less than the minimum detection limit to 4,511 mg/L (micrograms per liter) for benzene, 15,594 mg/L for toluene, 16,501 mg/L for ethylbenzene, and 19,391 mg/L for total xylenes. Concentrations of Total Petroleum Hydrocarbons-Gasoline Range Organics ranged from 323 mg/L to 3,364 mg/L; Total Petroleum Hydrocarbons-Diesel Range Organics were not detected. Three samples from this site were analyzed for benzene, toluene, ethylbenzene, and total xylenes at a laboratory and results showed concentrations ranging from less than the minimum detection limit to 1,070 mg/L for benzene, 7,930 mg/L for toluene, 6,890 mg/L for ethylbenzene, and 1,524 mg/L for total xylenes. Petroleum hydro- carbons were detected by on-site analysis in only one sample at Site 2438. A concentration of 131,000 micrograms per kilogram Total Petroleum Hydrocarbons-Diesel Range Organics was detected in sample number GP-2-4-13.5. Petroleum hydrocarbons were detected by on-site analysis in only one ground-water sample from Site 2444. A concentration of 3,145 mg/L Total Petroleum Hydrocarbons-Gasoline Range Organics was detected at sampling location GP-3-2.

Determining the minimum ripening time of artisanal Minas cheese, a traditional Brazilian cheese

PubMed Central

Martins, José M.; Galinari, Éder; Pimentel-Filho, Natan J.; Ribeiro, José I.; Furtado, Mauro M.; Ferreira, Célia L.L.F.

2015-01-01

Physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal Minas cheese from eight producers from Serro region (Minas Gerais, Brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by Brazilian legislation. The cheeses were produced between dry season (April–September) and rainy season (October–March); 128 cheeses were ripened at room temperature (25 ± 4 °C), and 128 were ripened under refrigeration (8 ± 1 °C), as a control. No Listeria monocytogenes was found, but one cheese under refrigeration had Salmonella at first 15 days of ripening. However, after 22 days, the pathogen was not detected. Seventeen days was the minimum ripening time at room temperature to reduce at safe limits of total coliforms > 1000 cfu.g −1 ), Escherichia coli and Staphylococcus aureus (> 100 cfu.g −1 ) in both periods of manufacture. Otherwise under refrigeration, as expected, the minimum ripening time was longer, 33 days in the dry season and 63 days in the rainy season. To sum up, we suggest that the ripening of artisanal Minas cheese be done at room temperature, since this condition shortens the time needed to reach the microbiological quality that falls within the safety parameters required by Brazilian law, and at the same time maintain the appearance and flavor characteristics of this traditional cheese. PMID:26221111

Covariance of lucky images: performance analysis

NASA Astrophysics Data System (ADS)

Cagigal, Manuel P.; Valle, Pedro J.; Cagigas, Miguel A.; Villó-Pérez, Isidro; Colodro-Conde, Carlos; Ginski, C.; Mugrauer, M.; Seeliger, M.

2017-01-01

The covariance of ground-based lucky images is a robust and easy-to-use algorithm that allows us to detect faint companions surrounding a host star. In this paper, we analyse the relevance of the number of processed frames, the frames' quality, the atmosphere conditions and the detection noise on the companion detectability. This analysis has been carried out using both experimental and computer-simulated imaging data. Although the technique allows us the detection of faint companions, the camera detection noise and the use of a limited number of frames reduce the minimum detectable companion intensity to around 1000 times fainter than that of the host star when placed at an angular distance corresponding to the few first Airy rings. The reachable contrast could be even larger when detecting companions with the assistance of an adaptive optics system.

Resolution Limits of Nanoimprinted Patterns by Fluorescence Microscopy

NASA Astrophysics Data System (ADS)

Kubo, Shoichi; Tomioka, Tatsuya; Nakagawa, Masaru

2013-06-01

The authors investigated optical resolution limits to identify minimum distances between convex lines of fluorescent dye-doped nanoimprinted resist patterns by fluorescence microscopy. Fluorescent ultraviolet (UV)-curable resin and thermoplastic resin films were transformed into line-and-space patterns by UV nanoimprinting and thermal nanoimprinting, respectively. Fluorescence immersion observation needed an immersion medium immiscible to the resist films, and an ionic liquid of triisobutyl methylphosphonium tosylate was appropriate for soluble thermoplastic polystyrene patterns. Observation with various numerical aperture (NA) values and two detection wavelength ranges showed that the resolution limits were smaller than the values estimated by the Sparrow criterion. The space width to identify line patterns became narrower as the line width increased. The space width of 100 nm was demonstrated to be sufficient to resolve 300-nm-wide lines in the detection wavelength range of 575-625 nm using an objective lens of NA= 1.40.

Mini-column assay for rapid detection of malachite green in fish.

PubMed

Shalaby, Ali R; Emam, Wafaa H; Anwar, Mervat M

2017-07-01

A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples. Copyright © 2017 Elsevier Ltd. All rights reserved.

Highly sensitive detection of naphthalene in solvent vapor using a functionalized PBG refractive index sensor.

PubMed

Girschikofsky, Maiko; Rosenberger, Manuel; Belle, Stefan; Brutschy, Malte; Waldvogel, Siegfried R; Hellmann, Ralf

2012-01-01

We report an optical refractive index sensor system based on a planar Bragg grating which is functionalized by substituted γ-cyclodextrin to determine low concentrations of naphthalene in solvent vapor. The sensor system exhibits a quasi-instantaneous shift of the Bragg wavelength and is therefore capable for online detection. The overall shift of the Bragg wavelength reveals a linear relationship to the analyte concentration with a gradient of 12.5 ± 1.5 pm/ppm. Due to the spectral resolution and repeatability of the interrogation system, this corresponds to acquisition steps of 80 ppb. Taking into account the experimentally detected signal noise a minimum detection limit of 0.48 ± 0.05 ppm is deduced.

22 CFR 151.4 - Minimum limits for motor vehicle insurance.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Minimum limits for motor vehicle insurance. 151.4 Section 151.4 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.4 Minimum limits for motor vehicle...

22 CFR 151.4 - Minimum limits for motor vehicle insurance.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Minimum limits for motor vehicle insurance. 151.4 Section 151.4 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.4 Minimum limits for motor vehicle...

22 CFR 151.4 - Minimum limits for motor vehicle insurance.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Minimum limits for motor vehicle insurance. 151.4 Section 151.4 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.4 Minimum limits for motor vehicle...

22 CFR 151.4 - Minimum limits for motor vehicle insurance.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Minimum limits for motor vehicle insurance. 151.4 Section 151.4 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.4 Minimum limits for motor vehicle...

22 CFR 151.4 - Minimum limits for motor vehicle insurance.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Minimum limits for motor vehicle insurance. 151.4 Section 151.4 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.4 Minimum limits for motor vehicle...

Fundamentals and practice for ultrasensitive laser-induced fluorescence detection in microanalytical systems.

PubMed

Johnson, Mitchell E; Landers, James P

2004-11-01

Laser-induced fluorescence is an extremely sensitive method for detection in chemical separations. In addition, it is well-suited to detection in small volumes, and as such is widely used for capillary electrophoresis and microchip-based separations. This review explores the detailed instrumental conditions required for sub-zeptomole, sub-picomolar detection limits. The key to achieving the best sensitivity is to use an excitation and emission volume that is matched to the separation system and that, simultaneously, will keep scattering and luminescence background to a minimum. We discuss how this is accomplished with confocal detection, 90 degrees on-capillary detection, and sheath-flow detection. It is shown that each of these methods have their advantages and disadvantages, but that all can be used to produce extremely sensitive detectors for capillary- or microchip-based separations. Analysis of these capabilities allows prediction of the optimal means of achieving ultrasensitive detection on microchips.

Microbial mineralization of dichloroethene and vinyl chloride under hypoxic conditions

USGS Publications Warehouse

Bradley, Paul M.; Chapelle, Francis H.

2011-01-01

Mineralization of 14C-radiolabled vinyl chloride ([1,2-14C] VC) and cis-dichloroethene ([1,2-14C] cis-DCE) under hypoxic (initial dissolved oxygen (DO) concentrations about 0.1 mg/L) and nominally anoxic (DO minimum detection limit = 0.01 mg/L) was examined in chloroethene-exposed sediments from two groundwater and two surface water sites. The results show significant VC and dichloroethene (DCE) mineralization under hypoxic conditions. All the sample treatments exhibited pseudo-first-order kinetics for DCE and VC mineralization over an extended range of substrate concentrations. First-order rates for VC mineralization were approximately 1 to 2 orders of magnitude higher in hypoxic groundwater sediment treatments and at least three times higher in hypoxic surface water sediment treatments than in the respective anoxic treatments. For VC, oxygen-linked processes accounted for 65 to 85% of mineralization at DO concentrations below 0.1 mg/L, and 14CO2 was the only degradation product observed in VC treatments under hypoxic conditions. Because the lower detection limit for DO concentrations measured in the field is typically 0.1 to 0.5 mg/L, these results indicate that oxygen-linked VC and DCE biodegradation can be significant under field conditions that appear anoxic. Furthermore, because rates of VC mineralization exceeded rates of DCE mineralization under hypoxic conditions, DCE accumulation without concomitant accumulation of VC may not be evidence of a DCE degradative “stall” in chloroethene plumes. Significantly, mineralization of VC above the level that could reasonably be attributed to residual DO contamination was also observed in several nominally anoxic (DO minimum detection limit = 0.01 mg/L) microcosm treatments.

Multipass open-path Fourier-transform infrared measurements for nonintrusive monitoring of gas turbine exhaust composition.

PubMed

Schäfer, Klaus; Brockmann, Klaus; Heland, Jörg; Wiesen, Peter; Jahn, Carsten; Legras, Olivier

2005-04-10

The detection limits for NO and NO2 in turbine exhausts by nonintrusive monitoring have to be improved. Multipass mode Fourier-transform infrared (FTIR) absorption spectrometry and use of a White mirror system were found from a sensitivity study with spectra simulations in the mid-infrared to be essential for the retrieval of NO2 abundances. A new White mirror system with a parallel infrared beam was developed and tested successfully with a commercial FTIR spectrometer in different turbine test beds. The minimum detection limits for a typical turbine plume of 50 cm in diameter are approximately 6 parts per million (ppm) for NO and 9 ppm for NO2 (as well 100 ppm for CO2 and 4 ppm for CO).

A System for Multiplexed Direct Electrical Detection of DNA Synthesis.

PubMed

Anderson, Erik P; Daniels, Jonathan S; Yu, Heng; Karhanek, Miloslav; Lee, Thomas H; Davis, Ronald W; Pourmand, Nader

2008-01-29

An electronic system for the multiplexed detection of DNA polymerization is designed and characterized. DNA polymerization is detected by the measurement of small transient currents arising from ion diffusion during polymerization. A transimpedance amplifier is used to detect these small currents; we implemented a twenty-four channel recording system on a single printed circuit board. Various contributions to the input-referred current noise are analyzed and characterized, as it limits the minimum detectable current and thus the biological limit of detection. We obtained 8.5 pA RMS mean noise current (averaged over all 24 channels) over the recording bandwidth (DC to 2 kHz). With digital filtering, the input-referred current noise of the acquisition system is reduced to 2.4 pA, which is much lower than the biological noise. Electrical crosstalk between channels is measured, and a model for the crosstalk is presented. Minimizing the crosstalk is critical because it can lead to erroneous microarray data. With proper precautions, crosstalk is reduced to a negligible value (less than 1.4%). Using a micro-fabricated array of 24 gold electrodes, we demonstrated system functionality by detecting the presence of a target DNA oligonucleotide which hybridized onto its corresponding target.

Three-Dimensional Distribution of Larval Fish Habitats in the Shallow Oxygen Minimum Zone in the Eastern Tropical Pacific Ocean off Mexico

NASA Astrophysics Data System (ADS)

Davies, S.; Sanchez Velasco, L.; Beier, E.; Godinez, V. M.; Barton, E. D.; Tamayo, A.

2016-02-01

Three-dimensional distribution of larval fish habitats was analyzed, from the upper limit of the shallow oxygen minimum zone ( 0.2 mL/L) to the sea surface, in the eastern tropical Pacific Ocean off Mexico in February 2010.The upper limit rises from 250 m depth in the entrance of the Gulf of California to 80 m depth off Cabo Corrientes. Three larval fish habitats were defined statistically: (i) a Gulf of California habitat dominated by Anchoa spp. larvae (epipelagic species), constrained to the oxygenated surface layer (>3.5 mL/L) in and above the thermocline ( 60 m depth), and separated by a salinity front from the Tropical Pacific habitat; (ii) a Tropical Pacific habitat, dominated by Vinciguerria lucetia larvae (mesopelagic species), located throughout the sampled water column, but with the highest abundance in the oxygenated upper layer above the thermocline; (iii) an Oxygen Minimum habitat defined mostly below the thermocline in hypoxic (<1 mL/L; 70 m depth) and anoxic (<0.2 mL/L; 80 m depth) water off Cabo Corrientes. This subsurface hypoxic habitat had the highest species richness and larval abundance, with dominance of Bregmaceros bathymaster, an endemic neritic pelagic species; which was an unexpected result. This maybe associated with the shoaling of the upper limit of the shallow oxygen minimum zone near the coast, a result of the strong costal upwelling detected by the Bakun Index. In this region of strong and semi-continuous coastal upwelling in the eastern tropical Pacific off Mexico, the shallow hypoxic water does not have dramatic effects on the total larval fish abundance but appears to affect species composition.

22 CFR 151.10 - Minimum limits of insurance for aircraft and/or vessels.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Minimum limits of insurance for aircraft and/or vessels. 151.10 Section 151.10 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.10 Minimum limits of insurance for...

22 CFR 151.10 - Minimum limits of insurance for aircraft and/or vessels.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Minimum limits of insurance for aircraft and/or vessels. 151.10 Section 151.10 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.10 Minimum limits of insurance for...

22 CFR 151.10 - Minimum limits of insurance for aircraft and/or vessels.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Minimum limits of insurance for aircraft and/or vessels. 151.10 Section 151.10 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.10 Minimum limits of insurance for...

22 CFR 151.10 - Minimum limits of insurance for aircraft and/or vessels.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Minimum limits of insurance for aircraft and/or vessels. 151.10 Section 151.10 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.10 Minimum limits of insurance for...

22 CFR 151.10 - Minimum limits of insurance for aircraft and/or vessels.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Minimum limits of insurance for aircraft and/or vessels. 151.10 Section 151.10 Foreign Relations DEPARTMENT OF STATE DIPLOMATIC PRIVILEGES AND IMMUNITIES COMPULSORY LIABILITY INSURANCE FOR DIPLOMATIC MISSIONS AND PERSONNEL § 151.10 Minimum limits of insurance for...

50 CFR 622.492 - Minimum size limit.

Code of Federal Regulations, 2013 CFR

2013-10-01

... ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Queen Conch Resources of Puerto Rico and the U.S. Virgin Islands § 622.492 Minimum size limit. (a) The minimum size...

50 CFR 622.492 - Minimum size limit.

Code of Federal Regulations, 2014 CFR

2014-10-01

... ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Queen Conch Resources of Puerto Rico and the U.S. Virgin Islands § 622.492 Minimum size limit. (a) The minimum size...

50 CFR 622.454 - Minimum size limit.

Code of Federal Regulations, 2013 CFR

2013-10-01

... ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Spiny Lobster Fishery of Puerto Rico and the U.S. Virgin Islands § 622.454 Minimum size limit. (a) The minimum...

50 CFR 622.454 - Minimum size limit.

Code of Federal Regulations, 2014 CFR

2014-10-01

... ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Spiny Lobster Fishery of Puerto Rico and the U.S. Virgin Islands § 622.454 Minimum size limit. (a) The minimum...

Muskellunge growth potential in northern Wisconsin: implications for trophy management

USGS Publications Warehouse

Faust, Matthew D.; Isermann, Daniel A.; Luehring, Mark A.; Hansen, Michael J.

2015-01-01

The growth potential of Muskellunge Esox masquinongy was evaluated by back-calculating growth histories from cleithra removed from 305 fish collected during 1995–2011 to determine whether it was consistent with trophy management goals in northern Wisconsin. Female Muskellunge had a larger mean asymptotic length (49.8 in) than did males (43.4 in). Minimum ultimate size of female Muskellunge (45.0 in) equaled the 45.0-in minimum length limit, but was less than the 50.0-in minimum length limit used on Wisconsin's trophy waters, while the minimum ultimate size of male Muskellunge (34.0 in) was less than the statewide minimum length limit. Minimum reproductive sizes for both sexes were less than Wisconsin's trophy minimum length limits. Mean growth potential of female Muskellunge in northern Wisconsin appears to be sufficient for meeting trophy management objectives and angler expectations. Muskellunge in northern Wisconsin had similar growth potential to those in Ontario populations, but lower growth potential than Minnesota's populations, perhaps because of genetic and environmental differences.

Multispecies breath analysis faster than a single respiratory cycle by optical-feedback cavity-enhanced absorption spectroscopy

NASA Astrophysics Data System (ADS)

Ventrillard-Courtillot, Irene; Gonthiez, Thierry; Clerici, Christine; Romanini, Daniel

2009-11-01

We demonstrate a first application, of optical-feedback cavity-enhanced absorption spectroscopy (OF-CEAS) to breath analysis in a medical environment. Noninvasive monitoring of trace species in exhaled air was performed simultaneous to spirometric measurements on patients at Bichat Hospital (Paris). The high selectivity of the OF-CEAS spectrometer and a time response of 0.3 s (limited by sample flow rate) allowed following the evolution of carbon monoxide and methane concentrations during individual respiratory cycles, and resolving variations among different ventilatory patterns. The minimum detectable absorption on this time scale is about 3×10-10 cm-1. At the working wavelength of the instrument (2.326 μm), this translates to concentration detection limits of ~1 ppbv (45 picomolar, or ~1.25 μg/m3) for CO and 25 ppbv for CH4, well below concentration values found in exhaled air. This same instrument is also able to provide measurement of NH3 concentrations with a detection limit of ~10 ppbv however, at present, memory effects do not allow its measurement on fast time scales.

Time and frequency constrained sonar signal design for optimal detection of elastic objects.

PubMed

Hamschin, Brandon; Loughlin, Patrick J

2013-04-01

In this paper, the task of model-based transmit signal design for optimizing detection is considered. Building on past work that designs the spectral magnitude for optimizing detection, two methods for synthesizing minimum duration signals with this spectral magnitude are developed. The methods are applied to the design of signals that are optimal for detecting elastic objects in the presence of additive noise and self-noise. Elastic objects are modeled as linear time-invariant systems with known impulse responses, while additive noise (e.g., ocean noise or receiver noise) and acoustic self-noise (e.g., reverberation or clutter) are modeled as stationary Gaussian random processes with known power spectral densities. The first approach finds the waveform that preserves the optimal spectral magnitude while achieving the minimum temporal duration. The second approach yields a finite-length time-domain sequence by maximizing temporal energy concentration, subject to the constraint that the spectral magnitude is close (in a least-squares sense) to the optimal spectral magnitude. The two approaches are then connected analytically, showing the former is a limiting case of the latter. Simulation examples that illustrate the theory are accompanied by discussions that address practical applicability and how one might satisfy the need for target and environmental models in the real-world.

Reflective measurement of water concentration using millimeter wave illumination

NASA Astrophysics Data System (ADS)

Sung, Shijun; Bennett, David; Taylor, Zachary; Bajwa, Neha; Tewari, Priyamvada; Maccabi, Ashkan; Culjat, Martin; Singh, Rahul; Grundfest, Warren

2011-04-01

THz and millimeter wave technology have shown the potential to become a valuable medical imaging tool because of its sensitivity to water and safe, non-ionizing photon energy. Using the high dielectric constant of water in these frequency bands, reflectionmode THz sensing systems can be employed to measure water content in a target with high sensitivity. This phenomenology may lead to the development of clinical systems to measure the hydration state of biological targets. Such measurements may be useful in fast and convenient diagnosis of conditions whose symptoms can be characterized by changes in water concentration such as skin burns, dehydration, or chemical exposure. To explore millimeter wave sensitivity to hydration, a reflectometry system is constructed to make water concentration measurements at 100 GHz, and the minimum detectable water concentration difference is measured. This system employs a 100 GHz Gunn diode source and Golay cell detector to perform point reflectivity measurements of a wetted polypropylene towel as it dries on a mass balance. A noise limited, minimum detectable concentration difference of less than 0.5% by mass can be detected in water concentrations ranging from 70% to 80%. This sensitivity is sufficient to detect hydration changes caused by many diseases and pathologies and may be useful in the future as a diagnostic tool for the assessment of burns and other surface pathologies.

Rare Earth Optical Temperature Sensor

NASA Technical Reports Server (NTRS)

Chubb, Donald L.; Wolford, David S.

2000-01-01

A new optical temperature sensor suitable for high temperatures (greater than 1700 K) and harsh environments is introduced. The key component of the sensor is the rare earth material contained at the end of a sensor that is in contact with the sample being measured. The measured narrow wavelength band emission from the rare earth is used to deduce the sample temperature. A simplified relation between the temperature and measured radiation was verified experimentally. The upper temperature limit of the sensor is determined by material limits to be approximately 2000 C. The lower limit, determined by the minimum detectable radiation, is found to be approximately 700 K. At high temperatures 1 K resolution is predicted. Also, millisecond response times are calculated.

Use of FTA sampling cards for molecular detection of avian influenza virus in wild birds.

PubMed

Keeler, Shamus P; Ferro, Pamela J; Brown, Justin D; Fang, Xingwang; El-Attrache, John; Poulson, Rebecca; Jackwood, Mark W; Stallknecht, David E

2012-03-01

Current avian influenza (AI) virus surveillance programs involving wild birds rely on sample collection methods that require refrigeration or low temperature freezing to maintain sample integrity for virus isolation and/or reverse-transcriptase (RT) PCR. Maintaining the cold chain is critical for the success of these diagnostic assays but is not always possible under field conditions. The aim of this study was to test the utility of Finders Technology Associates (FTA) cards for reliable detection of AI virus from cloacal and oropharyngeal swabs of wild birds. The minimum detectable titer was determined, and the effect of room temperature storage was evaluated experimentally using multiple egg-propagated stock viruses (n = 6). Using real time RT-PCR, we compared results from paired cloacal swab and samples collected on FTA cards from both experimentally infected mallards (Anasplatyrhynchos) and hunter-harvested waterfowl sampled along the Texas Gulf Coast. Based on the laboratory trials, the average minimal detectable viral titer was determined to be 1 x 10(4.7) median embryo infectious dose (EID50)/ml (range: 1 x 10(4.3) to 1 x 10(5.4) EID50/ml), and viral RNA was consistently detectable on the FTA cards for a minimum of 20 days and up to 30 days for most subtypes at room temperature (23 C) storage. Real-time RT-PCR of samples collected using the FTA cards showed fair to good agreement in live birds when compared with both real-time RT-PCR and virus isolation of swabs. AI virus detection rates in samples from several wild bird species were higher when samples were collected using the FTA cards compared with cloacal swabs. These results suggest that FTA cards can be used as an alternative sample collection method when traditional surveillance methods are not possible, especially in avian populations that have historically received limited testing or situations in which field conditions limit the ability to properly store or ship swab samples.

Enhancing interferometer phase estimation, sensing sensitivity, and resolution using robust entangled states

NASA Astrophysics Data System (ADS)

Smith, James F.

2017-11-01

With the goal of designing interferometers and interferometer sensors, e.g., LADARs with enhanced sensitivity, resolution, and phase estimation, states using quantum entanglement are discussed. These states include N00N states, plain M and M states (PMMSs), and linear combinations of M and M states (LCMMS). Closed form expressions for the optimal detection operators; visibility, a measure of the state's robustness to loss and noise; a resolution measure; and phase estimate error, are provided in closed form. The optimal resolution for the maximum visibility and minimum phase error are found. For the visibility, comparisons between PMMSs, LCMMS, and N00N states are provided. For the minimum phase error, comparisons between LCMMS, PMMSs, N00N states, separate photon states (SPSs), the shot noise limit (SNL), and the Heisenberg limit (HL) are provided. A representative collection of computational results illustrating the superiority of LCMMS when compared to PMMSs and N00N states is given. It is found that for a resolution 12 times the classical result LCMMS has visibility 11 times that of N00N states and 4 times that of PMMSs. For the same case, the minimum phase error for LCMMS is 10.7 times smaller than that of PMMS and 29.7 times smaller than that of N00N states.

Optimization and validation of moving average quality control procedures using bias detection curves and moving average validation charts.

PubMed

van Rossum, Huub H; Kemperman, Hans

2017-02-01

To date, no practical tools are available to obtain optimal settings for moving average (MA) as a continuous analytical quality control instrument. Also, there is no knowledge of the true bias detection properties of applied MA. We describe the use of bias detection curves for MA optimization and MA validation charts for validation of MA. MA optimization was performed on a data set of previously obtained consecutive assay results. Bias introduction and MA bias detection were simulated for multiple MA procedures (combination of truncation limits, calculation algorithms and control limits) and performed for various biases. Bias detection curves were generated by plotting the median number of test results needed for bias detection against the simulated introduced bias. In MA validation charts the minimum, median, and maximum numbers of assay results required for MA bias detection are shown for various bias. Their use was demonstrated for sodium, potassium, and albumin. Bias detection curves allowed optimization of MA settings by graphical comparison of bias detection properties of multiple MA. The optimal MA was selected based on the bias detection characteristics obtained. MA validation charts were generated for selected optimal MA and provided insight into the range of results required for MA bias detection. Bias detection curves and MA validation charts are useful tools for optimization and validation of MA procedures.

Minimum Detectable Activity for Tomographic Gamma Scanning System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Venkataraman, Ram; Smith, Susan; Kirkpatrick, J. M.

2015-01-01

For any radiation measurement system, it is useful to explore and establish the detection limits and a minimum detectable activity (MDA) for the radionuclides of interest, even if the system is to be used at far higher values. The MDA serves as an important figure of merit, and often a system is optimized and configured so that it can meet the MDA requirements of a measurement campaign. The non-destructive assay (NDA) systems based on gamma ray analysis are no exception and well established conventions, such the Currie method, exist for estimating the detection limits and the MDA. However, the Tomographicmore » Gamma Scanning (TGS) technique poses some challenges for the estimation of detection limits and MDAs. The TGS combines high resolution gamma ray spectrometry (HRGS) with low spatial resolution image reconstruction techniques. In non-imaging gamma ray based NDA techniques measured counts in a full energy peak can be used to estimate the activity of a radionuclide, independently of other counting trials. However, in the case of the TGS each “view” is a full spectral grab (each a counting trial), and each scan consists of 150 spectral grabs in the transmission and emission scans per vertical layer of the item. The set of views in a complete scan are then used to solve for the radionuclide activities on a voxel by voxel basis, over 16 layers of a 10x10 voxel grid. Thus, the raw count data are not independent trials any more, but rather constitute input to a matrix solution for the emission image values at the various locations inside the item volume used in the reconstruction. So, the validity of the methods used to estimate MDA for an imaging technique such as TGS warrant a close scrutiny, because the pair-counting concept of Currie is not directly applicable. One can also raise questions as to whether the TGS, along with other image reconstruction techniques which heavily intertwine data, is a suitable method if one expects to measure samples whose activities are at or just above MDA levels. The paper examines methods used to estimate MDAs for a TGS system, and explores possible solutions that can be rigorously defended.« less

Patterns of Song across Natural and Anthropogenic Soundscapes Suggest That White-Crowned Sparrows Minimize Acoustic Masking and Maximize Signal Content.

PubMed

Derryberry, Elizabeth P; Danner, Raymond M; Danner, Julie E; Derryberry, Graham E; Phillips, Jennifer N; Lipshutz, Sara E; Gentry, Katherine; Luther, David A

2016-01-01

Soundscapes pose both evolutionarily recent and long-standing sources of selection on acoustic communication. We currently know more about the impact of evolutionarily recent human-generated noise on communication than we do about how natural sounds such as pounding surf have shaped communication signals over evolutionary time. Based on signal detection theory, we hypothesized that acoustic phenotypes will vary with both anthropogenic and natural background noise levels and that similar mechanisms of cultural evolution and/or behavioral flexibility may underlie this variation. We studied song characteristics of white-crowned sparrows (Zonotrichia leucophrys nuttalli) across a noise gradient that includes both anthropogenic and natural sources of noise in San Francisco and Marin counties, California, USA. Both anthropogenic and natural soundscapes contain high amplitude low frequency noise (traffic or surf, respectively), so we predicted that birds would produce songs with higher minimum frequencies in areas with higher amplitude background noise to avoid auditory masking. We also anticipated that song minimum frequencies would be higher than the projected lower frequency limit of hearing based on site-specific masking profiles. Background noise was a strong predictor of song minimum frequency, both within a local noise gradient of three urban sites with the same song dialect and cultural evolutionary history, and across the regional noise gradient, which encompasses 11 urban and rural sites, several dialects, and several anthropogenic and natural sources of noise. Among rural sites alone, background noise tended to predict song minimum frequency, indicating that urban sites were not solely responsible for driving the regional pattern. These findings support the hypothesis that songs vary with local and regional soundscapes regardless of the source of noise. Song minimum frequency from five core study sites was also higher than the lower frequency limit of hearing at each site, further supporting the hypothesis that songs vary to transmit through noise in local soundscapes. Minimum frequencies leveled off at noisier sites, suggesting that minimum frequencies are constrained to an upper limit, possibly to retain the information content of wider bandwidths. We found evidence that site noise was a better predictor of song minimum frequency than territory noise in both anthropogenic and natural soundscapes, suggesting that cultural evolution rather than immediate behavioral flexibility is responsible for local song variation. Taken together, these results indicate that soundscapes shape song phenotype across both evolutionarily recent and long-standing soundscapes.

Patterns of Song across Natural and Anthropogenic Soundscapes Suggest That White-Crowned Sparrows Minimize Acoustic Masking and Maximize Signal Content

PubMed Central

Derryberry, Graham E.; Phillips, Jennifer N.; Lipshutz, Sara E.; Gentry, Katherine; Luther, David A.

2016-01-01

Soundscapes pose both evolutionarily recent and long-standing sources of selection on acoustic communication. We currently know more about the impact of evolutionarily recent human-generated noise on communication than we do about how natural sounds such as pounding surf have shaped communication signals over evolutionary time. Based on signal detection theory, we hypothesized that acoustic phenotypes will vary with both anthropogenic and natural background noise levels and that similar mechanisms of cultural evolution and/or behavioral flexibility may underlie this variation. We studied song characteristics of white-crowned sparrows (Zonotrichia leucophrys nuttalli) across a noise gradient that includes both anthropogenic and natural sources of noise in San Francisco and Marin counties, California, USA. Both anthropogenic and natural soundscapes contain high amplitude low frequency noise (traffic or surf, respectively), so we predicted that birds would produce songs with higher minimum frequencies in areas with higher amplitude background noise to avoid auditory masking. We also anticipated that song minimum frequencies would be higher than the projected lower frequency limit of hearing based on site-specific masking profiles. Background noise was a strong predictor of song minimum frequency, both within a local noise gradient of three urban sites with the same song dialect and cultural evolutionary history, and across the regional noise gradient, which encompasses 11 urban and rural sites, several dialects, and several anthropogenic and natural sources of noise. Among rural sites alone, background noise tended to predict song minimum frequency, indicating that urban sites were not solely responsible for driving the regional pattern. These findings support the hypothesis that songs vary with local and regional soundscapes regardless of the source of noise. Song minimum frequency from five core study sites was also higher than the lower frequency limit of hearing at each site, further supporting the hypothesis that songs vary to transmit through noise in local soundscapes. Minimum frequencies leveled off at noisier sites, suggesting that minimum frequencies are constrained to an upper limit, possibly to retain the information content of wider bandwidths. We found evidence that site noise was a better predictor of song minimum frequency than territory noise in both anthropogenic and natural soundscapes, suggesting that cultural evolution rather than immediate behavioral flexibility is responsible for local song variation. Taken together, these results indicate that soundscapes shape song phenotype across both evolutionarily recent and long-standing soundscapes. PMID:27128443

GIS-based niche modeling for mapping species' habitats

USGS Publications Warehouse

Rotenberry, J.T.; Preston, K.L.; Knick, S.

2006-01-01

Ecological a??niche modelinga?? using presence-only locality data and large-scale environmental variables provides a powerful tool for identifying and mapping suitable habitat for species over large spatial extents. We describe a niche modeling approach that identifies a minimum (rather than an optimum) set of basic habitat requirements for a species, based on the assumption that constant environmental relationships in a species' distribution (i.e., variables that maintain a consistent value where the species occurs) are most likely to be associated with limiting factors. Environmental variables that take on a wide range of values where a species occurs are less informative because they do not limit a species' distribution, at least over the range of variation sampled. This approach is operationalized by partitioning Mahalanobis D2 (standardized difference between values of a set of environmental variables for any point and mean values for those same variables calculated from all points at which a species was detected) into independent components. The smallest of these components represents the linear combination of variables with minimum variance; increasingly larger components represent larger variances and are increasingly less limiting. We illustrate this approach using the California Gnatcatcher (Polioptila californica Brewster) and provide SAS code to implement it.

Entanglement of 3000 atoms by detecting one photon

NASA Astrophysics Data System (ADS)

Vuletic, Vladan

2016-05-01

Quantum-mechanically correlated (entangled) states of many particles are of interest in quantum information, quantum computing and quantum metrology. In particular, entangled states of many particles can be used to overcome limits on measurements performed with ensembles of independent atoms (standard quantum limit). Metrologically useful entangled states of large atomic ensembles (spin squeezed states) have been experimentally realized. These states display Gaussian spin distribution functions with a non-negative Wigner quasiprobability distribution function. We report the generation of entanglement in a large atomic ensemble via an interaction with a very weak laser pulse; remarkably, the detection of a single photon prepares several thousand atoms in an entangled state. We reconstruct a negative-valued Wigner function, and verify an entanglement depth (the minimum number of mutually entangled atoms) that comprises 90% of the atomic ensemble containing 3100 atoms. Further technical improvement should allow the generation of more complex Schrödinger cat states, and of states the overcome the standard quantum limit.

A recoil-proton spectrometer based on a p-i-n diode implementing pulse-shape discrimination.

PubMed

Agosteo, S; D'Angelo, G; Fazzi, A; Foglio Para, A; Pola, A; Ventura, L; Zotto, P

2004-01-01

A recoil-proton spectrometer was created by coupling a p-i-n diode with a polyethylene converter. The maximum detectable energy, imposed by the thickness of the totally depleted layer, is approximately 6 MeV. The minimum detectable energy is limited by the contribution of secondary electrons generated by photons in the detector assembly. This limit is approximately 1.5 MeV at full-depletion voltage and was decreased using pulse-shape discrimination. The diode was set up in the 'reverse-injection' configuration (i.e. with the N+ layer adjacent to the converter). This configuration provides longer collection times for the electron-hole pairs generated by the recoil-protons. The pulse-shape discrimination was based on the zero-crossing time of bipolar signals from a (CR)2-(RC)2 filter. The detector was characterised using monoenergetic neutrons generated in the Van De Graaff CN accelerator at the INFN-Laboratori Nazionali di Legnaro. The energy limit for discrimination proved to be approximately 900 keV.

Multidetection of antibiotics in liver tissue by ultra-high-pressure-liquid-chromatography-tandem mass spectrometry.

PubMed

Freitas, Andreia; Barbosa, Jorge; Ramos, Fernando

2015-01-22

A multiresidue quantitative screening method covering 39 antibiotics from 7 different families by ultra-high-pressure-liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. A simple sample treatment method consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA) followed by solid-phase extraction (SPE) with a hydrophilic-lipophilic balanced (HLB) cartridge was developed. The methodology was validated, in accordance with Decision 2002/657/EC, by evaluating the following required parameters: decision limit (CCα), detection capability (CCβ), specificity, repeatability and reproducibility. The precision, in terms of the relative standard deviation, was under 22% for all of the compounds, and the recoveries were between 80% and 110%. The CCα and CCβ were determined according to the maximum residue limit (MRL) or the minimum required performance limit (MRPL), when established. Copyright © 2014 Elsevier B.V. All rights reserved.

Zinc phthalocyanine nanowires based flexible sensor for room temperature Cl2 detection

NASA Astrophysics Data System (ADS)

Devi, Pooja; Saini, Rajan; Singh, Rajinder; Mahajan, A.; Bedi, R. K.; Aswal, D. K.; Debnath, A. K.

2018-04-01

We have fabricated highly sensitive and Cl2 selective flexible sensor by depositing solution processed zinc phthalocyanine nanowires onto the flexible PET substrate and studied its Cl2 sensing characteristics in Cl2 concentration range 5-1500 ppb. The flexible sensor has a minimum detection limit as low as 5 ppb of Cl2 and response as high as 550% within 10 seconds. Interestingly, the sensor exhibited enhanced and faster response kinetics under bending conditions. The gas sensing mechanism of sensor has been discussed on the basis of XPS and Raman spectroscopic studies which revealed that zinc ions were the preferred sites for Cl2 interactions.

Two-IMU FDI performance of the sequential probability ratio test during shuttle entry

NASA Technical Reports Server (NTRS)

Rich, T. M.

1976-01-01

Performance data for the sequential probability ratio test (SPRT) during shuttle entry are presented. Current modeling constants and failure thresholds are included for the full mission 3B from entry through landing trajectory. Minimum 100 percent detection/isolation failure levels and a discussion of the effects of failure direction are presented. Finally, a limited comparison of failures introduced at trajectory initiation shows that the SPRT algorithm performs slightly worse than the data tracking test.

Quartz-enhanced photoacoustic detection of ethylene using a 10.5 μm quantum cascade laser.

PubMed

Wang, Zhen; Li, Zhili; Ren, Wei

2016-02-22

A quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor has been developed for the sensitive detection of ethylene (C2H4) at 10.5 µm using a continuous-wave distributed-feedback quantum cascade laser. At this long-wavelength infrared, the key acoustic elements of quartz tuning fork and micro-resonators were optimized to improve the detection signal-to-noise ratio by a factor of >4. The sensor calibration demonstrated an excellent linear response (R2>0.999) to C2H4 concentration at the selected operating pressure of 500 and 760 Torr. With a minimum detection limit of 50 parts per billion (ppb) achieved at an averaging time of 70 s, the sensor has been deployed for measuring the C2H4 efflux during the respiration of biological samples in an agronomic environment.

Seasonal temperature variation around the mesopause inferred from a VHF meteor radar at King Sejong Station (62S, 59W), Antarctica

NASA Astrophysics Data System (ADS)

Kim, Yongha; Kim, Jeong-Han; Lee, Changsup; Jee, Gun-Hwa

A VHF meteor radar, installed at King Sejong Station in March, 2007, has been detecting echoes from more than 20,000 meteors per day. Meteor echoes are decayed typically within seconds as meteors spread away by atmospheric diffusion. The diffusion coefficients can thus be obtained from decay times of meteor echo signals, providing with information on the atmospheric temperatures and pressures at meteor altitudes from 70 to 100 km. In this study, we present altitude profiles of 15-min averaged diffusion coefficients in each month, which clearly show a minimum at 80 - 85 km. The minimum appears at higher altitude during austral summer than winter, and seems to be near the lower level of two temperature minimum structure around the mesopause seen by TIMED/SABER data at high latitudes. The higher mesopause level (95-100 km) of the SABER data does not appear in our diffusion profiles probably because it is too close the limit of meaningful diffusion coefficients that can be derived from meteor decay detection. In order to understand temperature variation around the mesopause more directly, we will discuss various methods to extract temperature profiles from the diffusion profiles. We will also present monthly averaged OH and O2 airglow temperatures observed at the same site, and compare them with those derived from the meteor radar observation.

Heterogeneity, histological features and DNA ploidy in oral carcinoma by image-based analysis.

PubMed

Diwakar, N; Sperandio, M; Sherriff, M; Brown, A; Odell, E W

2005-04-01

Oral squamous carcinomas appear heterogeneous on DNA ploidy analysis. However, this may be partly a result of sample dilution or the detection limit of techniques. The aim of this study was to determine whether oral squamous carcinomas are heterogeneous for ploidy status using image-based ploidy analysis and to determine whether ploidy status correlates with histological parameters. Multiple samples from 42 oral squamous carcinomas were analysed for DNA ploidy using an image-based system and scored for histological parameters. 22 were uniformly aneuploid, 1 uniformly tetraploid and 3 uniformly diploid. 16 appeared heterogeneous but only 8 appeared to be genuinely heterogeneous when minor ploidy histogram peaks were taken into account. Ploidy was closely related to nuclear pleomorphism but not differentiation. Sample variation, detection limits and diagnostic criteria account for much of the ploidy heterogeneity observed. Confident diagnosis of diploid status in an oral squamous cell carcinoma requires a minimum of 5 samples.

Colors of extreme exo-Earth environments.

PubMed

Hegde, Siddharth; Kaltenegger, Lisa

2013-01-01

The search for extrasolar planets has already detected rocky planets and several planetary candidates with minimum masses that are consistent with rocky planets in the habitable zone of their host stars. A low-resolution spectrum in the form of a color-color diagram of an exoplanet is likely to be one of the first post-detection quantities to be measured for the case of direct detection. In this paper, we explore potentially detectable surface features on rocky exoplanets and their connection to, and importance as, a habitat for extremophiles, as known on Earth. Extremophiles provide us with the minimum known envelope of environmental limits for life on our planet. The color of a planet reveals information on its properties, especially for surface features of rocky planets with clear atmospheres. We use filter photometry in the visible as a first step in the characterization of rocky exoplanets to prioritize targets for follow-up spectroscopy. Many surface environments on Earth have characteristic albedos and occupy a different color space in the visible waveband (0.4-0.9 μm) that can be distinguished remotely. These detectable surface features can be linked to the extreme niches that support extremophiles on Earth and provide a link between geomicrobiology and observational astronomy. This paper explores how filter photometry can serve as a first step in characterizing Earth-like exoplanets for an aerobic as well as an anaerobic atmosphere, thereby prioritizing targets to search for atmospheric biosignatures.

A reversed-phase compatible thin-layer chromatography autography for the detection of acetylcholinesterase inhibitors.

PubMed

Ramallo, I Ayelen; García, Paula; Furlan, Ricardo L E

2015-11-01

A dual readout autographic assay to detect acetylcholinesterase inhibitors present in complex matrices adsorbed on reversed-phase or normal-phase thin-layer chromatography plates is described. Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The effects of substrate and enzyme concentrations, pH, incubation time, and incubation temperature on the sensitivity and the detection limit of the assay were evaluated. Experimental design and response surface methodology were used to optimize conditions with a minimum number of experiments. The assay allowed the detection of 0.01% w/w of physostigmine in both a spiked Sonchus oleraceus L. extract chromatographed on normal phase and a spiked Pimenta racemosa (Mill.) J.W. Moore leaf essential oil chromatographed on reversed phase. Finally, the reversed-phase thin-layer chromatography assay was applied to reveal the presence of an inhibitor in the Cymbopogon citratus (DC.) Stapf essential oil. The developed assay is able to detect acetylcholinesterase inhibitors present in complex matrixes that were chromatographed in normal phase or reversed-phase thin-layer chromatography. The detection limit for physostigmine on both normal and reversed phase was of 1×10(-4) μg. The results can be read by a change in color and/or a change in fluorescence. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Exploration of the association rules mining technique for the signal detection of adverse drug events in spontaneous reporting systems.

PubMed

Wang, Chao; Guo, Xiao-Jing; Xu, Jin-Fang; Wu, Cheng; Sun, Ya-Lin; Ye, Xiao-Fei; Qian, Wei; Ma, Xiu-Qiang; Du, Wen-Min; He, Jia

2012-01-01

The detection of signals of adverse drug events (ADEs) has increased because of the use of data mining algorithms in spontaneous reporting systems (SRSs). However, different data mining algorithms have different traits and conditions for application. The objective of our study was to explore the application of association rule (AR) mining in ADE signal detection and to compare its performance with that of other algorithms. Monte Carlo simulation was applied to generate drug-ADE reports randomly according to the characteristics of SRS datasets. Thousand simulated datasets were mined by AR and other algorithms. On average, 108,337 reports were generated by the Monte Carlo simulation. Based on the predefined criterion that 10% of the drug-ADE combinations were true signals, with RR equaling to 10, 4.9, 1.5, and 1.2, AR detected, on average, 284 suspected associations with a minimum support of 3 and a minimum lift of 1.2. The area under the receiver operating characteristic (ROC) curve of the AR was 0.788, which was equivalent to that shown for other algorithms. Additionally, AR was applied to reports submitted to the Shanghai SRS in 2009. Five hundred seventy combinations were detected using AR from 24,297 SRS reports, and they were compared with recognized ADEs identified by clinical experts and various other sources. AR appears to be an effective method for ADE signal detection, both in simulated and real SRS datasets. The limitations of this method exposed in our study, i.e., a non-uniform thresholds setting and redundant rules, require further research.

Demonstration and Validation of a Regenerated Cellulose Dialysis Membrane Diffusion Sampler for Monitoring Ground Water Quality and Remediation Progress at DoD Sites for Perchlorate and Explosives Compounds

DTIC Science & Technology

2010-09-01

Regulatory Council LRL Laboratory reporting level LDPE Low-density polyethylene MDL Minimum detection limit MNA Monitored natural attenuation...consists of a tubular-shaped bag made of flexible low-density polyethylene ( LDPE ) (Vroblesky, 2001a, 2001b). The LDPE tube is heat-sealed on one end...be constructed from small- diameter LDPE tubing that fits into small-diameter wells. These polyethylene diffusion bag (PDB) samplers have been

Demonstration and Validation of a Regenerated Cellulose Dialysis Membrane Diffusion Sampler for Monitoring Ground-Water Quality and Remediation Progress at DoD Site: Perchlorate and Ordnance Compounds

DTIC Science & Technology

2011-10-01

Regulatory Council LDPE low-density polyethylene MDL minimum detection limit NAVFAC ESC Naval Facilities Engineering Command Engineering Service...membrane sampler design consists of a tubular-shaped bag made of flexible low-density polyethylene ( LDPE ) (Vroblesky, 2001a, 2001b). The LDPE tube is...requirements, and can be constructed from small-diameter LDPE tubing that fits into small- 4 diameter wells. These polyethylene diffusion bag

Demonstration and Validation of a Regenerated Cellulose Dialysis Membrane Diffusion Sampler for Monitoring Ground-Water Quality and Remediation Progress at DoD Sites

DTIC Science & Technology

2010-04-01

LDPE low-density polyethylene LF low-flow purging LRL laboratory reporting level MDL minimum detection limit MNA monitored natural attenuation...shaped bag made of flexible low-density polyethylene ( LDPE ) (Vroblesky, 2001a, 2001b). The LDPE tube is heat-sealed on one end, filled with high...from small- diameter LDPE tubing that fits into small-diameter wells. These PDB samplers have been shown to be useful only for collection of VOCs

Mid-Infrared Trace Gas Sensor Technology Based on Intracavity Quartz-Enhanced Photoacoustic Spectroscopy.

PubMed

Wojtas, Jacek; Gluszek, Aleksander; Hudzikowski, Arkadiusz; Tittel, Frank K

2017-03-04

The application of compact inexpensive trace gas sensor technology to a mid-infrared nitric oxide (NO) detectoion using intracavity quartz-enhanced photoacoustic spectroscopy (I-QEPAS) is reported. A minimum detection limit of 4.8 ppbv within a 30 ms integration time was demonstrated by using a room-temperature, continuous-wave, distributed-feedback quantum cascade laser (QCL) emitting at 5.263 µm (1900.08 cm -1 ) and a new compact design of a high-finesse bow-tie optical cavity with an integrated resonant quartz tuning fork (QTF). The optimum configuration of the bow-tie cavity was simulated using custom software. Measurements were performed with a wavelength modulation scheme (WM) using a 2f detection procedure.

Water-quality data for the Talkeetna River and four streams in National Parks, Cook Inlet basin, Alaska, 1998

USGS Publications Warehouse

Frenzel, Steven A.; Dorava, Joseph M.

1999-01-01

Five streams in the Cook Inlet Basin, Alaska, were sampled in 1998 to provide the National Park Service with baseline information on water quality. Four of these streams drain National Park Service land: Costello and Colorado Creeks in Denali National Park and Preserve, Johnson River in Lake Clark National Park and Preserve, and Kamishak River in Katmai National Park and Preserve. The fifth site was on the Talkeetna River, outside of national park boundaries. Samples of stream water, streambed sediments, and fish tissues were collected for chemical analyses. Biological and geomorphic information was also collected at each site. Nutrient concentrations in stream water were low and commonly were less than analytical detection limits. Analyses of fish tissues for 28 organochlorine compounds at Talkeetna River and Costello Creek produced just one detection. Hexachlorobenzene was detected at a concentration of 5.70 micrograms per kilogram in slimy sculpin from the Talkeetna River. Streambed sediment samples from the Talkeetna River had three organochlorine compounds at detectable levels; hexachlorobenzene was measured at 13 micrograms per kilogram and two other compounds were below the minimum reporting levels. At Colorado Creek, Johnson River, and Kamishak River, where fish samples were not collected, no organochlorine compounds were detected in streambed sediment samples. Several semivolatile organic compounds were detected at Colorado Creek and Costello Creek. Only one compound, dibenzothiophene, detected at Costello Creek at a concentration of 85 micrograms per kilogram was above the minimum reporting limit. No semivolatile organic compounds were detected at the Talkeetna, Kamishak, or Johnson Rivers. Trace elements were detected in both fish tissues and streambed sediments. Macroinvertebrate and fish samples contained few taxa at all sites. Total numbers of macroinvertebrate taxa ranged from 19 at the Johnson River to 38 at the Talkeetna River. Diptera were the most abundant and diverse order of macroinvertebrates at all sites. Total numbers of diptera taxa ranged from 8 at the Kamishak River to 19 at the Talkeetna River. Fish communities were represented by a maximum of nine taxa at the Talkeetna River and were absent at Colorado Creek. The Johnson River sampling site produced small numbers of juvenile Dolly Varden, and Costello Creek produced small numbers of both juvenile Dolly Varden and slimy sculpin.

Evaluation of Beckman Coulter DxI 800 immunoassay system using clinically oriented performance goals.

PubMed

Akbas, Neval; Schryver, Patricia G; Algeciras-Schimnich, Alicia; Baumann, Nikola A; Block, Darci R; Budd, Jeffrey R; Gaston, S J Stephen; Klee, George G

2014-11-01

We evaluated the analytical performance of 24 immunoassays using the Beckman Coulter DxI 800 immunoassay systems at Mayo Clinic, Rochester, MN for trueness, precision, detection limits, linearity, and consistency (across instruments and reagent lots). Clinically oriented performance goals were defined using the following methods: trueness-published desirable accuracy limits, precision-published desirable biologic variation; detection limits - 0.1 percentile of patient test values, linearity - 50% of total error, and consistency-percentage test values crossing key decision points. Local data were collected for precision, linearity, and consistency. Data were provided by Beckman Coulter, Inc. for trueness and detection limits. All evaluated assays except total thyroxine were within the proposed goals for trueness. Most of the assays met the proposed goals for precision (86% of intra-assay results and 75% of inter-assay results). Five assays had more than 15% of the test results below the minimum detection limits. Carcinoembryonic antigen, total thyroxine and free triiodothyronine exceeded the proposed goals of ±6.3%, ±5% and ±5.7% for dilution linearity. All evaluated assays were within the proposed goals for instrument consistency. Lot-to-lot consistency results for cortisol, ferritin and total thyroxine exceeded the proposed goals of 3.3%, 11.4% and 7% at one medical decision level, while vitamin B12 exceeded the proposed goals of 5.2% and 3.8% at two decision levels. The Beckman Coulter DxI 800 immunoassay system meets most of these proposed goals, even though these clinically focused performance goals represent relatively stringent limits. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Development of an X-ray surface analyzer for planetary exploration

NASA Technical Reports Server (NTRS)

Clark, B. C.

1972-01-01

An ultraminiature X-ray fluorescence spectrometer was developed which can obtain data on element composition not provided by present spacecraft instrumentation. The apparatus employs two radioisotope sources (Fe-55 and Cd-109) which irradiate adjacent areas on a soil sample. Fluorescent X-rays emitted by the sample are detected by four thin-window proportional counters. Using pulse-height discrimination, the energy spectra are determined. Virtually all elements above sodium in the periodic table are detected if present at sufficient levels. Minimum detection limits range from 30 ppm to several percent, depending upon the element and the matrix. For most elements, they are below 0.5 percent. Accuracies likewise depend upon the matrix, but are generally better than plus or minus 0.5 percent for all elements of atomic number greater than 14. Elements below sodium are also detected, but as a single group.

Quantum-statistical theory of microwave detection using superconducting tunnel junctions

NASA Astrophysics Data System (ADS)

Deviatov, I. A.; Kuzmin, L. S.; Likharev, K. K.; Migulin, V. V.; Zorin, A. B.

1986-09-01

A quantum-statistical theory of microwave and millimeter-wave detection using superconducting tunnel junctions is developed, with a rigorous account of quantum, thermal, and shot noise arising from fluctuation sources associated with the junctions, signal source, and matching circuits. The problem of the noise characterization in the quantum sensitivity range is considered and a general noise parameter Theta(N) is introduced. This parameter is shown to be an adequate figure of merit for most receivers of interest while some devices can require a more complex characterization. Analytical expressions and/or numerically calculated plots for Theta(N) are presented for the most promising detection modes including the parametric amplification, heterodyne mixing, and quadratic videodetection, using both the quasiparticle-current and the Cooper-pair-current nonlinearities. Ultimate minimum values of Theta(N) for each detection mode are compared and found to be in agreement with limitations imposed by the quantum-mechanical uncertainty principle.

Of Detection Limits and Effective Mitigation: The Use of Infrared Cameras for Methane Leak Detection

NASA Astrophysics Data System (ADS)

Ravikumar, A. P.; Wang, J.; McGuire, M.; Bell, C.; Brandt, A. R.

2017-12-01

Mitigating methane emissions, a short-lived and potent greenhouse gas, is critical to limiting global temperature rise to two degree Celsius as outlined in the Paris Agreement. A major source of anthropogenic methane emissions in the United States is the oil and gas sector. To this effect, state and federal governments have recommended the use of optical gas imaging systems in periodic leak detection and repair (LDAR) surveys to detect for fugitive emissions or leaks. The most commonly used optical gas imaging systems (OGI) are infrared cameras. In this work, we systematically evaluate the limits of infrared (IR) camera based OGI system for use in methane leak detection programs. We analyze the effect of various parameters that influence the minimum detectable leak rates of infrared cameras. Blind leak detection tests were carried out at the Department of Energy's MONITOR natural gas test-facility in Fort Collins, CO. Leak sources included natural gas wellheads, separators, and tanks. With an EPA mandated 60 g/hr leak detection threshold for IR cameras, we test leak rates ranging from 4 g/hr to over 350 g/hr at imaging distances between 5 ft and 70 ft from the leak source. We perform these experiments over the course of a week, encompassing a wide range of wind and weather conditions. Using repeated measurements at a given leak rate and imaging distance, we generate detection probability curves as a function of leak-size for various imaging distances, and measurement conditions. In addition, we estimate the median detection threshold - leak-size at which the probability of detection is 50% - under various scenarios to reduce uncertainty in mitigation effectiveness. Preliminary analysis shows that the median detection threshold varies from 3 g/hr at an imaging distance of 5 ft to over 150 g/hr at 50 ft (ambient temperature: 80 F, winds < 4 m/s). Results from this study can be directly used to improve OGI based LDAR protocols and reduce uncertainty in estimated mitigation effectiveness. Furthermore, detection limits determined in this study can be used as standards to compare new detection technologies.

Predicting subtle behavioral responses of invertebrates to soil contaminants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Donkin, S.G.

1995-12-31

At concentration levels well below those which cause death and injury to soil invertebrates, a toxic chemical plume may yet effectively damage a soil ecosystem by triggering avoidance behavior among sensitive invertebrates as they move along the concentration gradient. The result may be a soil ecosystem lacking the benefits of effective nutrient cycling and mineralization which a thriving invertebrate population provides. While determining actual detection limits of invertebrates for chemical gradients in soils is experimentally difficult, theoretical calculations have suggested that such limits may be extremely low, and hence many organisms may sense and avoid concentrations of chemicals far belowmore » levels commonly considered acceptable. The minimum gradient (G) that can be detected by a receptor depends on the receptor radius (R), the chemical concentration (C), the diffusion constant of the chemical (D), the velocity of the organism (v), and the time over which the receptor integrates the chemical signal (t). In addition, the characteristics of that gradient are determined by interactions between the chemical and the soil particles (sorption/desorption), and advection through the pore spaces. The example of lead (Pb), a neurotoxic metal with demonstrated behavioral effects on the free-living nematode Caenorhabditis elegans, is used to model a chemical migrating through a soil. Based on experimentally determined Pb concentrations which elicited avoidance behavior in nematodes, and sorption characteristics of defined Pb-soil systems, the minimum detectable gradient (G) produced by a solubilized Pb plume in several soils was modeled. The results predict maximum allowable Pb levels in a soil if a healthy invertebrate community is desired, and suggest areas for further research into the subtle behavioral effects of environmental toxicants ore sensitive invertebrates.« less

VCSEL based Faraday rotation spectroscopy at 762nm for battery powered trace molecular oxygen detection

NASA Astrophysics Data System (ADS)

So, Stephen; Wysocki, Gerard

2010-02-01

Faraday Rotation Spectroscopy (FRS) is a polarization based spectroscopic technique which can provide higher sensitivity concentration measurements of paramagnetic gases and free radicals than direct absorption spectroscopic techniques. We have developed sensor systems which require only 0.2W to perform TDLAS (tunable diode laser absorption spectroscopy), and can additionally be quickly duty cycled, enabling operation in wireless sensor networks of laser-based trace gas sensors We adapted our integrated TDLAS electronics to perform FRS in a compact and more sensitive system for quantification of molecular oxygen (O2) using a 762.3nm VCSEL in the A band. Using an AC magnetic field, we demonstrate detector noise dominated performance, achieving 2.1×10-6/Hz1/2 equivalent detectable fractional absorption and a minimum detection limit of 462 ppmv O2 in 1 second in a 15cm path. At longer paths and integration times, such a sensor will enable oxygen measurements at biotic respiration levels (<1ppmv) to measure CO2 - O2 exchange for mapping natural exchange of greenhouse gases. Potential improvement of detection limits by increasing various system performance parameters is described.

Determination of plutonium isotopes (238,239,240Pu) and strontium (90Sr) in seafood using alpha spectrometry and liquid scintillation spectrometry.

PubMed

Shin, Choonshik; Choi, Hoon; Kwon, Hye-Min; Jo, Hye-Jin; Kim, Hye-Jeong; Yoon, Hae-Jung; Kim, Dong-Sul; Kang, Gil-Jin

2017-10-01

The present study was carried out to survey the levels of plutonium isotopes ( 238 , 239 , 240 Pu) and strontium ( 90 Sr) in domestic seafood in Korea. In current, regulatory authorities have analyzed radionuclides, such as 134 Cs, 137 Cs and 131 I, in domestic and imported food. However, people are concerned about contamination of other radionuclides, such as plutonium and strontium, in food. Furthermore, people who live in Korea have much concern about safety of seafood. Accordingly, in this study, we have investigated the activity concentrations of plutonium and strontium in seafood. For the analysis of plutonium isotopes and strontium, a rapid and reliable method developed from previous study was used. Applicability of the test method was verified by examining recovery, minimum detectable activity (MDA), analytical time, etc. Total 40 seafood samples were analyzed in 2014-2015. As a result, plutonium isotopes ( 238 , 239 , 240 Pu) and strontium ( 90 Sr) were not detected or below detection limits in seafood. The detection limits of plutonium isotopes and strontium-90 were 0.01 and 1 Bq/kg, respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

Internal validation of the GlobalFiler™ Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow.

PubMed

Flores, Shahida; Sun, Jie; King, Jonathan; Budowle, Bruce

2014-05-01

The GlobalFiler™ Express PCR Amplification Kit uses 6-dye fluorescent chemistry to enable multiplexing of 21 autosomal STRs, 1 Y-STR, 1 Y-indel and the sex-determining marker amelogenin. The kit is specifically designed for processing reference DNA samples in a high throughput manner. Validation studies were conducted to assess the performance and define the limitations of this direct amplification kit for typing blood and buccal reference DNA samples on various punchable collection media. Studies included thermal cycling sensitivity, reproducibility, precision, sensitivity of detection, minimum detection threshold, system contamination, stochastic threshold and concordance. Results showed that optimal amplification and injection parameters for a 1.2mm punch from blood and buccal samples were 27 and 28 cycles, respectively, combined with a 12s injection on an ABI 3500xL Genetic Analyzer. Minimum detection thresholds were set at 100 and 120RFUs for 27 and 28 cycles, respectively, and it was suggested that data from positive amplification controls provided a better threshold representation. Stochastic thresholds were set at 250 and 400RFUs for 27 and 28 cycles, respectively, as stochastic effects increased with cycle number. The minimum amount of input DNA resulting in a full profile was 0.5ng, however, the optimum range determined was 2.5-10ng. Profile quality from the GlobalFiler™ Express Kit and the previously validated AmpFlSTR(®) Identifiler(®) Direct Kit was comparable. The validation data support that reliable DNA typing results from reference DNA samples can be obtained using the GlobalFiler™ Express PCR Amplification Kit. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

The impact of registration accuracy on imaging validation study design: A novel statistical power calculation.

PubMed

Gibson, Eli; Fenster, Aaron; Ward, Aaron D

2013-10-01

Novel imaging modalities are pushing the boundaries of what is possible in medical imaging, but their signal properties are not always well understood. The evaluation of these novel imaging modalities is critical to achieving their research and clinical potential. Image registration of novel modalities to accepted reference standard modalities is an important part of characterizing the modalities and elucidating the effect of underlying focal disease on the imaging signal. The strengths of the conclusions drawn from these analyses are limited by statistical power. Based on the observation that in this context, statistical power depends in part on uncertainty arising from registration error, we derive a power calculation formula relating registration error, number of subjects, and the minimum detectable difference between normal and pathologic regions on imaging, for an imaging validation study design that accommodates signal correlations within image regions. Monte Carlo simulations were used to evaluate the derived models and test the strength of their assumptions, showing that the model yielded predictions of the power, the number of subjects, and the minimum detectable difference of simulated experiments accurate to within a maximum error of 1% when the assumptions of the derivation were met, and characterizing sensitivities of the model to violations of the assumptions. The use of these formulae is illustrated through a calculation of the number of subjects required for a case study, modeled closely after a prostate cancer imaging validation study currently taking place at our institution. The power calculation formulae address three central questions in the design of imaging validation studies: (1) What is the maximum acceptable registration error? (2) How many subjects are needed? (3) What is the minimum detectable difference between normal and pathologic image regions? Copyright © 2013 Elsevier B.V. All rights reserved.

A One ppm NDIR Methane Gas Sensor with Single Frequency Filter Denoising Algorithm

PubMed Central

Zhu, Zipeng; Xu, Yuhui; Jiang, Binqing

2012-01-01

A non-dispersive infrared (NDIR) methane gas sensor prototype has achieved a minimum detection limit of 1 parts per million by volume (ppm). The central idea of the design of the sensor is to decrease the detection limit by increasing the signal to noise ratio (SNR) of the system. In order to decrease the noise level, a single frequency filter algorithm based on fast Fourier transform (FFT) is adopted for signal processing. Through simulation and experiment, it is found that the full width at half maximum (FWHM) of the filter narrows with the extension of sampling period and the increase of lamp modulation frequency, and at some optimum sampling period and modulation frequency, the filtered signal maintains a noise to signal ratio of below 1/10,000. The sensor prototype provides the key techniques for a hand-held methane detector that has a low cost and a high resolution. Such a detector may facilitate the detection of leakage of city natural gas pipelines buried underground, the monitoring of landfill gas, the monitoring of air quality and so on.

Analytical methods for multiresidue determination of sulfonamides and trimethoprim in meat and ground water samples by CE-MS and CE-MS/MS.

PubMed

Soto-Chinchilla, Jorge J; García-Campaña, Ana M; Gámiz-Gracia, Laura

2007-11-01

This paper presents two methods based on CZE-MS detection and CZE-MS/MS detection developed for the multiresidue determination of ten sulfonamides (sulfapyridine, sulfadoxin, sulfamethazine, sulfadimethoxine, sulfameter, sulfamerazine, sulfachlorpyridazine, sulfadiazine, sulfamethoxazole, and sulfamethizole) and a potentiator, trimethoprim (TMP), whose contents are regulated by the EU Council Regulation no. 2377/90 in animal edible tissues. Experimental designs were employed to optimize the electrospray conditions. MS/MS experiments using an IT as analyzer operating in multiple reaction monitoring (MRM) mode were carried out to achieve the minimum number of points according to the 2002/657/EC European Decision for unambiguous identification. The proposed procedures have been compared in terms of the performance characteristics and trueness. The limits of detection and quantification were in all cases lower than the maximum residue limits legislated for these compounds and the recoveries were satisfactory, being possible the application for their monitoring in foodstuff of animal origin and in environmental samples, allowing the determination of sulfonamides and TMP residues in meat and in superficial water in the low microg/L range.

Molecular interferometric imaging study of molecular interactions

NASA Astrophysics Data System (ADS)

Zhao, Ming; Wang, Xuefeng; Nolte, David

2008-02-01

Molecular Interferometric Imaging (MI2) is a sensitive detection platform for direct optical detection of immobilized biomolecules. It is based on inline common-path interferometry combined with far-field optical imaging. The substrate is a simple thermal oxide on a silicon surface with a thickness at or near the quadrature condition that produces a π/2 phase shift between the normal-incident wave reflected from the top oxide surface and the bottom silicon surface. The presence of immobilized or bound biomolecules on the surface produces a relative phase shift that is converted to a far-field intensity shift and is imaged by a reflective microscope onto a CCD camera. Shearing interferometry is used to remove the spatial 1/f noise from the illumination to achieve shot-noise-limited detection of surface dipole density profiles. The lateral resolution of this technique is diffraction limited at 0.4 micron, and the best longitudinal resolution is 10 picometers. The minimum detectable mass at the metrology limit is 2 attogram, which is 8 antibody molecules of size 150 kDa. The corresponding scaling mass sensitivity is 5 fg/mm compared with 1 pg/mm for typical SPR sensitivity. We have applied MI2 to immunoassay applications, and real-time binding kinetics has been measured for antibody-antigen reactions. The simplicity of the substrate and optical read-out make MI2 a promising analytical assay tool for high-throughput screening and diagnostics.

Dip-strip method for monitoring environmental contamination of aflatoxin in food and feed: use of a portable aflatoxin detection kit.

PubMed

Sashidhar, R B

1993-10-01

Aflatoxin contamination of food and feed have gained global significance due to its deleterious effect on human and animal health and its importance in the international trade. The potential of aflatoxin as a carcinogen, mutagen, teratogen, and immunosuppressive agent is well documented. The problem of aflatoxin contamination of food and feed has led to the enactment of various legislation. However, meaningful strategies for implementation of this legislation is limited by nonavailability of simple, cost-effective method for screening and detection of aflatoxin under field conditions. Keeping in mind the analytical constraints in developing countries, a simple-to-operate, rapid, reliable, and cost-effective portable aflatoxin detection kit has been developed. The important components of the kit include a hand-held UV lamp (365 nm, 4 W output), a solvent blender (12,000 rpm) for toxin extraction, and adsorbent-coated dip-strips (polyester film) for detecting and quantifying aflatoxin. Analysis of variance indicates that there were no significant differences between various batches of dip-strips (p > 0.05). The minimum detection limit for aflatoxin B1 was 10 ppb per spot. The kit may find wide application as a research tool in public health laboratories, environmental monitoring agencies, and in the poultry industry.

Dip-strip method for monitoring environmental contamination of aflatoxin in food and feed: use of a portable aflatoxin detection kit.

PubMed Central

Sashidhar, R B

1993-01-01

Aflatoxin contamination of food and feed have gained global significance due to its deleterious effect on human and animal health and its importance in the international trade. The potential of aflatoxin as a carcinogen, mutagen, teratogen, and immunosuppressive agent is well documented. The problem of aflatoxin contamination of food and feed has led to the enactment of various legislation. However, meaningful strategies for implementation of this legislation is limited by nonavailability of simple, cost-effective method for screening and detection of aflatoxin under field conditions. Keeping in mind the analytical constraints in developing countries, a simple-to-operate, rapid, reliable, and cost-effective portable aflatoxin detection kit has been developed. The important components of the kit include a hand-held UV lamp (365 nm, 4 W output), a solvent blender (12,000 rpm) for toxin extraction, and adsorbent-coated dip-strips (polyester film) for detecting and quantifying aflatoxin. Analysis of variance indicates that there were no significant differences between various batches of dip-strips (p > 0.05). The minimum detection limit for aflatoxin B1 was 10 ppb per spot. The kit may find wide application as a research tool in public health laboratories, environmental monitoring agencies, and in the poultry industry. Images FIGURE 1. PMID:8143644

Aflatoxin M1 Concentration in Various Dairy Products: Evidence for Biologically Reduced Amount of AFM1 in Yoghurt

PubMed Central

RAHIMIRAD, Amir; MAALEKINEJAD, Hassan; OSTADI, Araz; YEGANEH, Samal; FAHIMI, Samira

2014-01-01

Abstract Background Aflatoxin M1 (AFM1), a carcinogenic substance is found in milk and dairy products. The effect of season and type of dairy products on AFMi level in northern Iran was investigated in this study. Methods Three hundred samples (each season 75 samples) including raw and pasteurized milk, yoghurt, cheese, and cream samples were collected from three distinct milk producing farms. The samples were subjected to chemical and solid phase extractions and were analyzed by using HPLC technique. Recovery percentages, limit of detection and limit of quantification values were determined. Results Seventy percent and 98% were the minimum and maximum recoveries for cheese and raw milk, respectively and 0.021 and 0.063 ppb were the limit of detection and limit of quantification values for AFM1. We found that in autumn and winter the highest level (0.121 ppb) of AFM1 in cheese and cream samples and failed to detect any AFM1 in spring samples. Interestingly, our data showed that the yoghurt samples had the lowest level of AFM1 in all seasons. Conclusion There are significant differences between the AFM1 levels in dairy products in various seasons and also various types of products, suggesting spring and summer yoghurt samples as the safest products from AFM1 level point of view. PMID:25927044

A Limit on the Warm Dark Matter Particle Mass from the Redshifted 21 cm Absorption Line

NASA Astrophysics Data System (ADS)

Safarzadeh, Mohammadtaher; Scannapieco, Evan; Babul, Arif

2018-06-01

The recent Experiment to Detect the Global Epoch of Reionization Signature (EDGES) collaboration detection of an absorption signal at a central frequency of ν = 78 ± 1 MHz points to the presence of a significant Lyα background by a redshift of z = 18. The timing of this signal constrains the dark matter particle mass (m χ ) in the warm dark matter (WDM) cosmological model. WDM delays the formation of small-scale structures, and therefore a stringent lower limit can be placed on m χ based on the presence of a sufficiently strong Lyα background due to star formation at z = 18. Our results show that coupling the spin temperature to the gas through Lyα pumping requires a minimum mass of m χ > 3 keV if atomic cooling halos dominate the star formation rate at z = 18, and m χ > 2 keV if {{{H}}}2 cooling halos also form stars efficiently at this redshift. These limits match or exceed the most stringent limits cited to date in the literature, even in the face of the many uncertainties regarding star formation at high redshift.

Minimum length from quantum mechanics and classical general relativity.

PubMed

Calmet, Xavier; Graesser, Michael; Hsu, Stephen D H

2004-11-19

We derive fundamental limits on measurements of position, arising from quantum mechanics and classical general relativity. First, we show that any primitive probe or target used in an experiment must be larger than the Planck length lP. This suggests a Planck-size minimum ball of uncertainty in any measurement. Next, we study interferometers (such as LIGO) whose precision is much finer than the size of any individual components and hence are not obviously limited by the minimum ball. Nevertheless, we deduce a fundamental limit on their accuracy of order lP. Our results imply a device independent limit on possible position measurements.

Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) Project: Detect and Avoid Display Evaluations in Support of SC-228 Minimum Operational Performance Standards Development

NASA Technical Reports Server (NTRS)

Fern, Lisa Carolynn

2017-01-01

The primary activity for the UAS-NAS Human Systems Integration (HSI) sub-project in Phase 1 was support of RTCA Special Committee 228 Minimum Operational Performance Standards (MOPS). We provide data on the effect of various Detect and Avoid (DAA) display features with respect to pilot performance of the remain well clear function in order to determine the minimum requirements for DAA displays.

X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

PubMed Central

de Jonge, Martin D.; Ryan, Christopher G.; Jacobsen, Chris J.

2014-01-01

X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer. PMID:25177992

Ultrasensitive detection of nitric oxide at 5.33 μm by using external cavity quantum cascade laser-based Faraday rotation spectroscopy

PubMed Central

Lewicki, Rafał; Doty, James H.; Curl, Robert F.; Tittel, Frank K.; Wysocki, Gerard

2009-01-01

A transportable prototype Faraday rotation spectroscopic system based on a tunable external cavity quantum cascade laser has been developed for ultrasensitive detection of nitric oxide (NO). A broadly tunable laser source allows targeting the optimum Q3/2(3/2) molecular transition at 1875.81 cm−1 of the NO fundamental band. For an active optical path of 44 cm and 1-s lock-in time constant minimum NO detection limits (1σ) of 4.3 parts per billion by volume (ppbv) and 0.38 ppbv are obtained by using a thermoelectrically cooled mercury–cadmium–telluride photodetector and liquid nitrogen-cooled indium–antimonide photodetector, respectively. Laboratory performance evaluation and results of continuous, unattended monitoring of atmospheric NO concentration levels are reported. PMID:19625625

Intracavity Faraday modulation spectroscopy (INFAMOS): A tool for radical detection

NASA Astrophysics Data System (ADS)

Gianella, Michele; Pinto, Tomas H. P.; Wu, Xia; Ritchie, Grant A. D.

2017-08-01

We present the intra-cavity Faraday modulation spectroscopy technique, whereby optical feedback cavity-enhanced spectroscopy is coupled with Faraday modulation spectroscopy to greatly enhance the interaction path length of a laser beam with a paramagnetic sample in a magnetic field. We describe a first prototype based upon a cw quantum cascade laser targeting a selection of fundamental rovibrational R-branch transitions of nitric oxide (1890 cm-1), consisting of a linear cavity (finesse F =6300 ) and a water-cooled solenoid. We demonstrate a minimum detectable Verdet constant of Vmin=4.7 ×10-14 rad cm-1 G-1 H z-1/2 (at SNR = 1), corresponding to a single-pass rotation angle of 1.6 ×10-10 rad Hz-1/2 and a limit of detection of 0.21 ppbv Hz-1/2 NO.

THE NASA-UC ETA-EARTH PROGRAM. II. A PLANET ORBITING HD 156668 WITH A MINIMUM MASS OF FOUR EARTH MASSES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Howard, Andrew W.; Marcy, Geoffrey W.; Isaacson, Howard

2011-01-10

We report the discovery of HD 156668 b, an extrasolar planet with a minimum mass of M{sub P} sin i = 4.15 M{sub +}. This planet was discovered through Keplerian modeling of precise radial velocities from Keck-HIRES and is the second super-Earth to emerge from the NASA-UC Eta-Earth Survey. The best-fit orbit is consistent with circular and has a period of P = 4.6455 days. The Doppler semi-amplitude of this planet, K = 1.89 m s{sup -1}, is among the lowest ever detected, on par with the detection of GJ 581 e using HARPS. A longer period (P {approx} 2.3more » years), low-amplitude signal of unknown origin was also detected in the radial velocities and was filtered out of the data while fitting the short-period planet. Additional data are required to determine if the long-period signal is due to a second planet, stellar activity, or another source. Photometric observations using the Automated Photometric Telescopes at Fairborn Observatory show that HD 156668 (an old, quiet K3 dwarf) is photometrically constant over the radial velocity period to 0.1 mmag, supporting the existence of the planet. No transits were detected down to a photometric limit of {approx}3 mmag, ruling out transiting planets dominated by extremely bloated atmospheres, but not precluding a transiting solid/liquid planet with a modest atmosphere.« less

Paralarvae of the complex Sthenoteuthis oualaniensis-Dosidicus gigas (Cephalopoda: Ommastrephidae) in the northern limit of the shallow oxygen minimum zone of the Eastern Tropical Pacific Ocean (April 2012)

NASA Astrophysics Data System (ADS)

Sánchez-Velasco, Laura; Ruvalcaba-Aroche, Erick D.; Beier, Emilio; Godínez, Victor M.; Barton, Eric D.; Díaz-Viloria, Noe; Pacheco, María. R.

2016-03-01

The three-dimensional distribution of the paralarvae of the complex Sthenoteuthis oualaniensis-Dosidicus gigas (Cephalopoda: Ommastrephidae) was analyzed at the northern limit of the shallow oxygen minimum zone in the Eastern Tropical Pacific in April 2012. The upper limit of the oxygen minimum water (˜44 µmol/kg or 1 mL/L) rises from ˜100 m depth in the entrance of the Gulf of California to ˜20 m depth off Cabo Corrientes. Most of the paralarvae of this complex, dominated by D. gigas, were concentrated in the Gulf entrance, between the thermocline (˜20 to ˜50 m depth) and the sea surface, in the warmest (>19°C) oxygenated (>176 µmol/kg) layer. The highest abundance of paralarvae was detected in an anticyclonic eddy (˜120 km diameter and >500 m deep), which contained lower-salinity water (<35 g/kg), consistent with formation in the California Current. Lower paralarvae abundance was recorded further south off Cabo Corrientes, where hypoxic layers were elevated as water shoaled nearshore. Almost no paralarvae were found in the north of the study area beyond the strong salinity front (˜34.8-35.4 g/kg) that bounded the anticyclone. These results showed an affinity of the paralarvae for lower-salinity, oxygenated water, illustrated by the influence of the mesoscale anticyclonic eddy and the salinity front in their distribution. Based on this study, it can be concluded that the expansion of the depth range of hypoxic water observed in the Eastern Tropical Pacific may be increasing environmental stress on the paralarvae by vertically restricting their habitat, and so affecting their survival.

Improvement in QEPAS system utilizing a second harmonic based wavelength calibration technique

NASA Astrophysics Data System (ADS)

Zhang, Qinduan; Chang, Jun; Wang, Fupeng; Wang, Zongliang; Xie, Yulei; Gong, Weihua

2018-05-01

A simple laser wavelength calibration technique, based on second harmonic signal, is demonstrated in this paper to improve the performance of quartz enhanced photoacoustic spectroscopy (QEPAS) gas sensing system, e.g. improving the signal to noise ratio (SNR), detection limit and long-term stability. Constant current, corresponding to the gas absorption line, combining f/2 frequency sinusoidal signal are used to drive the laser (constant driving mode), a software based real-time wavelength calibration technique is developed to eliminate the wavelength drift due to ambient fluctuations. Compared to conventional wavelength modulation spectroscopy (WMS), this method allows lower filtering bandwidth and averaging algorithm applied to QEPAS system, improving SNR and detection limit. In addition, the real-time wavelength calibration technique guarantees the laser output is modulated steadily at gas absorption line. Water vapor is chosen as an objective gas to evaluate its performance compared to constant driving mode and conventional WMS system. The water vapor sensor was designed insensitive to the incoherent external acoustic noise by the numerical averaging technique. As a result, the SNR increases 12.87 times in wavelength calibration technique based system compared to conventional WMS system. The new system achieved a better linear response (R2 = 0 . 9995) in concentration range from 300 to 2000 ppmv, and achieved a minimum detection limit (MDL) of 630 ppbv.

Swept-source based, single-shot, multi-detectable velocity range Doppler optical coherence tomography

PubMed Central

Meemon, Panomsak; Rolland, Jannick P.

2010-01-01

Phase-Resolved Doppler Optical Coherence Tomography (PR-DOCT) allows visualization and characterization of the location, direction, velocity, and profile of flow activity embedded in a static sample structure. The detectable Velocity Dynamic Range (VDR) of each particular PR-DOCT system is governed by a detectable Doppler phase shift, a flow angle, and an acquisition time interval used to determine the Doppler phase shift. In general, the lower boundary of the detectable Doppler phase shift is limited by the phase stability of the system, while the upper boundary is limited by the π phase ambiguity. For a given range of detectable Doppler phase shift, shortening the acquisition duration will increase not only the maximum detectable velocity but unfortunately also the minimum detectable velocity, which may lead to the invisibility of a slow flow. In this paper, we present an alternative acquisition scheme for PR-DOCT that extends the lower limit of the velocity dynamic range, while maintaining the maximum detectable velocity, hence increasing the overall VDR of PR-DOCT system. The essence of the approach is to implement a technique of multi-scale measurement to simultaneously acquire multiple VDRs in a single measurement. We demonstrate an example of implementation of the technique in a dual VDR DOCT, where two Doppler maps having different detectable VDRs were simultaneously detected, processed, and displayed in real time. One was a fixed VDR DOCT capable of measuring axial velocity of up to 10.9 mm/s without phase unwrapping. The other was a variable VDR DOCT capable of adjusting its detectable VDR to reveal slow flow information down to 11.3 μm/s. The technique is shown to effectively extend the overall detectable VDR of the PR-DOCT system. Examples of real time Doppler imaging of an African frog tadpole are demonstrated using the dual-VDR DOCT system. PMID:21258521

Detection of cow milk adulteration in yak milk by ELISA.

PubMed

Ren, Q R; Zhang, H; Guo, H Y; Jiang, L; Tian, M; Ren, F Z

2014-10-01

In the current study, a simple, sensitive, and specific ELISA assay using a high-affinity anti-bovine β-casein monoclonal antibody was developed for the rapid detection of cow milk in adulterated yak milk. The developed ELISA was highly specific and could be applied to detect bovine β-casein (10-8,000 μg/mL) and cow milk (1:1,300 to 1:2 dilution) in yak milk. Cross-reactivity was <1% when tested against yak milk. The linear range of adulterant concentration was 1 to 80% (vol/vol) and the minimum detection limit was 1% (vol/vol) cow milk in yak milk. Different treatments, including heating, acidification, and rennet addition, did not interfere with the assay. Moreover, the results were highly reproducible (coefficient of variation <10%) and we detected no significant differences between known and estimated values. Therefore, this assay is appropriate for the routine analysis of yak milk adulterated with cow milk. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Detection and persistence of environmental DNA from an invasive, terrestrial mammal.

PubMed

Williams, Kelly E; Huyvaert, Kathryn P; Vercauteren, Kurt C; Davis, Amy J; Piaggio, Antoinette J

2018-01-01

Invasive Sus scrofa , a species commonly referred to as wild pig or feral swine, is a destructive invasive species with a rapidly expanding distribution across the United States. We used artificial wallows and small waterers to determine the minimum amount of time needed for pig eDNA to accumulate in the water source to a detectable level. We removed water from the artificial wallows and tested eDNA detection over the course of 2 weeks to understand eDNA persistence. We show that our method is sensitive enough to detect very low quantities of eDNA shed by a terrestrial mammal that has limited interaction with water. Our experiments suggest that the number of individuals shedding into a water system can affect persistence of eDNA. Use of an eDNA detection technique can benefit management efforts by providing a sensitive method for finding even small numbers of individuals that may be elusive using other methods.

Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription–Loop-Mediated Isothermal Amplification

PubMed Central

Zhang, Jinhai; Feng, Youjun; Hu, Dan; Lv, Heng; Zhu, Jing; Cao, Min; Zheng, Feng; Zhu, Jin; Gong, Xiufang; Hao, Lina; Srinivas, Swaminath; Ren, Hao; Qi, Zhongtian

2013-01-01

An epidemic of human H7N9 influenza virus infection recently emerged in China whose clinical features include high mortality and which has also resulted in serious economic loss. The novel reassortant avian-origin influenza A (H7N9) virus which was the causative agent of this epidemic raised the possibility of triggering a large-scale influenza pandemic worldwide. It seemed likely that fast molecular detection assays specific for this virus would be in great demand. Here, we report a one-step reverse transcription–loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of the hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 virus, the minimum detection limit of which was evaluated using in vitro RNA transcription templates. In total, 135 samples from clinical specimens (from either patients or poultry) were tested using this method in comparison with the real-time PCR recommended by the World Health Organization (WHO). Our results showed that (i) RT-LAMP-based trials can be completed in approximately 12 to 23 min and (ii) the detection limit for the H7 gene is around 10 copies per reaction, similar to that of the real-time PCR, whereas the detection limit for its counterpart the N9 gene is 5 copies per reaction, a 100-fold-higher sensitivity than the WHO-recommended method. Indeed, this excellent performance of our method was also validated by the results for a series of clinical specimens. Therefore, we believe that the simple, fast, and sensitive method of RT-LAMP might be widely applied for detection of H7N9 infections and may play a role in prevention of an influenza pandemic. PMID:24006004

Effects of Phasor Measurement Uncertainty on Power Line Outage Detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Chen; Wang, Jianhui; Zhu, Hao

2014-12-01

Phasor measurement unit (PMU) technology provides an effective tool to enhance the wide-area monitoring systems (WAMSs) in power grids. Although extensive studies have been conducted to develop several PMU applications in power systems (e.g., state estimation, oscillation detection and control, voltage stability analysis, and line outage detection), the uncertainty aspects of PMUs have not been adequately investigated. This paper focuses on quantifying the impact of PMU uncertainty on power line outage detection and identification, in which a limited number of PMUs installed at a subset of buses are utilized to detect and identify the line outage events. Specifically, the linemore » outage detection problem is formulated as a multi-hypothesis test, and a general Bayesian criterion is used for the detection procedure, in which the PMU uncertainty is analytically characterized. We further apply the minimum detection error criterion for the multi-hypothesis test and derive the expected detection error probability in terms of PMU uncertainty. The framework proposed provides fundamental guidance for quantifying the effects of PMU uncertainty on power line outage detection. Case studies are provided to validate our analysis and show how PMU uncertainty influences power line outage detection.« less

DMSK: A practical 2400-bps receiver for the mobile satellite service: An MSAT-X Report

NASA Technical Reports Server (NTRS)

Davarian, F.; Simon, M. K.; Sumida, J.

1985-01-01

The partical aspects of a 2400-bps differential detection minimum-shift-keying (DMSK) receiver are investigated. Fundamental issues relating to hardware precision, Doppler shift, fading, and frequency offset are examined, and it is concluded that the receiver's implementation at baseband is more advantageous both in cost and simplicity than its IF implementation. The DMSK receiver has been fabricated and tested under simulated mobile satellite environment conditions. The measured receiver performance in the presence of anomalies pertinent to the link is presented in this report. Furthermore, the receiver behavior in a band-limited channel (GMSK) is also investigated. The DMSK receiver performs substantially better than a coherent minimum-shift-keying (MSK) receiver in a heavily fading environment. The DMSK radio is simple and robust, and results in a lower error floor than its coherent counterpart. Moreover, this receiver is suitable for burst-type signals, and its recovery from deep fades is fast.

A portable detection system for in vivo monitoring of 131I in routine and emergency situations

NASA Astrophysics Data System (ADS)

Lucena, EA; Dantas, ALA; Dantas, BM

2018-03-01

In vivo monitoring of 131I in human thyroid is often used to evaluate occupational exposure in nuclear medicine facilities and in the case of accidental intakes in nuclear power plants for the monitoring of workers and population. The device presented in this work consists on a Pb-collimated NaI(Tl)3”x3” scintillation detector assembled on a tripod and connected to a portable PC. The evaluation of the applicability and limitations of the system is based on the estimation of the committed effective doses associated to the minimum detectable activities in different facilities. It has been demonstrated that the system is suitable for use in routine and accidental situations.

Mid-Infrared Trace Gas Sensor Technology Based on Intracavity Quartz-Enhanced Photoacoustic Spectroscopy

PubMed Central

Wojtas, Jacek; Gluszek, Aleksander; Hudzikowski, Arkadiusz; Tittel, Frank K.

2017-01-01

The application of compact inexpensive trace gas sensor technology to a mid-infrared nitric oxide (NO) detectoion using intracavity quartz-enhanced photoacoustic spectroscopy (I-QEPAS) is reported. A minimum detection limit of 4.8 ppbv within a 30 ms integration time was demonstrated by using a room-temperature, continuous-wave, distributed-feedback quantum cascade laser (QCL) emitting at 5.263 µm (1900.08 cm−1) and a new compact design of a high-finesse bow-tie optical cavity with an integrated resonant quartz tuning fork (QTF). The optimum configuration of the bow-tie cavity was simulated using custom software. Measurements were performed with a wavelength modulation scheme (WM) using a 2f detection procedure. PMID:28273836

Microbial Mineralization of cis-Dichloroethene and Vinyl Chloride as a Component of Natural Attenuation of Chloroethene Contaminants under Conditions Identified in the Field as Anoxic

DTIC Science & Technology

2012-01-01

oxygen. If a study site is predominated by highly reducing conditions, then laboratory experimental procedures that establish such conditions are...less than the 0.01 mg/L minimum detection limit for the gas chromatographic method employed in this study . Given the uncertainty in O2 concentrations... CO2 , 14CO2, ethene, 14C-ethene, ethane, 14C-ethane, and 14C-VC (in 14C-DCE treatments only) were monitored using packed column gas

14 CFR 25.1523 - Minimum flight crew.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Minimum flight crew. 25.1523 Section 25.1523 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... Limitations § 25.1523 Minimum flight crew. The minimum flight crew must be established so that it is...

14 CFR 29.1523 - Minimum flight crew.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Minimum flight crew. 29.1523 Section 29.1523 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... Limitations § 29.1523 Minimum flight crew. The minimum flight crew must be established so that it is...

Optical filter requirements in an EML-based single-sideband PAM4 intensity-modulation and direct-detection transmission system.

PubMed

Chen, Hsing-Yu; Kaneda, Noriaki; Lee, Jeffrey; Chen, Jyehong; Chen, Young-Kai

2017-03-20

The feasibility of a single sideband (SSB) PAM4 intensity-modulation and direct-detection (IM/DD) transmission based on a CMOS ADC and DAC is experimentally demonstrated in this work. To cost effectively build a >50 Gb/s system as well as to extend the transmission distance, a low cost EML and a passive optical filter are utilized to generate the SSB signal. However, the EML-induced chirp and dispersion-induced power fading limit the requirements of the SSB filter. To separate the effect of signal-signal beating interference, filters with different roll-off factors are employed to demonstrate the performance tolerance at different transmission distance. Moreover, a high resolution spectrum analysis is proposed to depict the system limitation. Experimental results show that a minimum roll-off factor of 7 dB/10GHz is required to achieve a 51.84Gb/s 40-km transmission with only linear feed-forward equalization.

Challenges in miniaturized automotive long-range lidar system design

NASA Astrophysics Data System (ADS)

Fersch, Thomas; Weigel, Robert; Koelpin, Alexander

2017-05-01

This paper discusses the current technical limitations posed on endeavors to miniaturize lidar systems for use in automotive applications and how to possibly extend those limits. The focus is set on long-range scanning direct time of flight LiDAR systems using APD photodetectors. Miniaturization evokes severe problems in ensuring absolute laser safety while maintaining the systems' performance in terms of maximum range, signal-to-noise ratio, detection probability, pixel density, or frame rate. Based on hypothetical but realistic specifications for an exemplary system the complete lidar signal path is calculated. The maximum range of the system is used as a general performance indicator. It is determined with the minimum signal-to-noise ratio required to detect an object. Various system parameters are varied to find their impact on the system's range. The reduction of the laser's pulse width and the right choice for the transimpedance amplifier's amplification have shown to be practicable measures to double the system's range.

Oxygen measurements at high pressures with vertical cavity surface-emitting lasers

NASA Astrophysics Data System (ADS)

Wang, J.; Sanders, S. T.; Jeffries, J. B.; Hanson, R. K.

Measurements of oxygen concentration at high pressures (to 10.9 bar) were made using diode-laser absorption of oxygen A-band transitions near 760 nm. The wide current-tuning frequency range (>30 cm-1) of vertical cavity surface-emitting lasers (VCSELs) was exploited to enable the first scanned-wavelength demonstration of diode-laser absorption at high pressures; this strategy is more robust than fixed-wavelength strategies, particularly in hostile environments. The wide tuning range and rapid frequency response of the current tuning were further exploited to demonstrate wavelength-modulation absorption spectroscopy in a high-pressure environment. The minimum detectable absorbance demonstrated, 1×10-4, corresponds to 800 ppm-m oxygen detectivity at room temperature and is limited by etalon noise. The rapid- and wide-frequency tunability of VCSELs should significantly expand the application domain of absorption-based sensors limited in the past by the small current-tuning frequency range (typically <2 cm-1) of conventional edge-emitting diode lasers.

Feedback damping of a microcantilever at room temperature to the minimum vibration amplitude limited by the noise level

PubMed Central

Kawamura, Y.; Kanegae, R.

2016-01-01

Cooling the vibration amplitude of a microcantilever as low as possible is important to improve the sensitivity and resolutions of various types of scanning type microscopes and sensors making use of it. When the vibration amplitude is controlled to be smaller using a feed back control system, it is known that the obtainable minimum amplitude of the vibration is limited by the floor noise level of the detection system. In this study, we demonstrated that the amplitude of the thermal vibration of a microcantilever was suppressed to be about 0.15 pmHz−1/2, which is the same value with the floor noise level, without the assistance of external cryogenic cooling. We think that one of the reason why we could reach the smaller amplitude at room temperature is due to stiffer spring constant of the lever, which leads to higher natural frequency and consequently lower floor noise level. The other reason is considered to be due to the increase in the laser power for the diagnostics, which lead to the decrease in the signal to noise ratio determined by the optical shot noise. PMID:27312284

14 CFR 25.1513 - Minimum control speed.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Minimum control speed. 25.1513 Section 25.1513 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... Limitations § 25.1513 Minimum control speed. The minimum control speed V MC determined under § 25.149 must be...

14 CFR 25.1513 - Minimum control speed.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Minimum control speed. 25.1513 Section 25.1513 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... Limitations § 25.1513 Minimum control speed. The minimum control speed V MC determined under § 25.149 must be...

Temporal modulation transfer functions in auditory receptor fibres of the locust ( Locusta migratoria L.).

PubMed

Prinz, P; Ronacher, B

2002-08-01

The temporal resolution of auditory receptors of locusts was investigated by applying noise stimuli with sinusoidal amplitude modulations and by computing temporal modulation transfer functions. These transfer functions showed mostly bandpass characteristics, which are rarely found in other species at the level of receptors. From the upper cut-off frequencies of the modulation transfer functions the minimum integration times were calculated. Minimum integration times showed no significant correlation to the receptor spike rates but depended strongly on the body temperature. At 20 degrees C the average minimum integration time was 1.7 ms, dropping to 0.95 ms at 30 degrees C. The values found in this study correspond well to the range of minimum integration times found in birds and mammals. Gap detection is another standard paradigm to investigate temporal resolution. In locusts and other grasshoppers application of this paradigm yielded values of the minimum detectable gap widths that are approximately twice as large than the minimum integration times reported here.

NO2 trace measurements by optical-feedback cavity-enhanced absorption spectroscopy

NASA Astrophysics Data System (ADS)

Ventrillard-Courtillot, I.; Foldes, T.; Romanini, D.

2009-04-01

In order to reach the sub-ppb NO2 detection level required for environmental applications in remote areas, we are developing a spectrometer that exploits a technique that we introduced several years ago, named Optical-Feedback Cavity-Enhanced Absorption Spectroscopy (OF-CEAS) [1]. It allows very sensitive and selective measurements, together with the realization of compact and robust set-ups as was subsequently demonstrated during measurements campaigns in harsh environments [2,3]. OF-CEAS benefits from the optical feedback (OF) to efficiently inject a cw-laser in a high finesse cavity (typically F >10 000). Absorption spectra are acquired on a small spectral region (~1 cm-1) that enables selective and quantitative measurements at a fast acquisition rate (~10 Hz) with a detection limit of several 10-10 cm-1 as reported in this paper. Spectra are obtained with high spectral resolution (~150 MHz) and are self calibrated by cavity rind-down measurements regularly performed (typically every second). Therefore, OF-CEAS appears very attractive for NO2 trace detection. This work is performed in the blue spectral region where NO2 has intense electronic transitions. Our setup involves a commercial extended cavity diode laser (ECDL) working at room temperature around 411nm. A first setup was developed [4] to demonstrate that OF sensitivity of ECDL is fully consistent with this technique, initially introduced with distributed feedback diode lasers in the near infrared region. In this paper we will report on a new set-up developed for in-situ measurements with proper mechanical, acoustic and thermal insulation. Additionally, new data processing was implemented allowing real time concentration measurements. It is based on a reference spectra recorded under controlled conditions by OF-CEAS and used later to fit the observed spectra. We will present measurements performed with calibrated NO2 reference samples demonstrating a good linearity of the apparatus. The minimum detectable absorption loss is estimated by considering the standard deviation of the spectra. We achieved better than 2x10-10 cm-1 for a single spectrum recorded in less than 100ms at 100mbar. This limit constitutes an improved of more than one order of magnitude as compare to the previous measurements reported in [4]. It leads to a detection limit of 3x108 molecules/cm3, corresponding to about 150pptv at 100mbar. At atmospheric pressure the same measurement would yield a detection limit of 15pptv assuming we can maintain the same level of sensitivity. But currently, works are under development to transfer the low minimum detectable absorption limit, already obtained for one spectrum, to a set of real time measurements. These are now limited by amplitude fluctuations of a few ppb from one spectrum to another one. [1] J. Morville, S. Kassi, M. Chenevier, and D. Romanini, Appl. Phys. B, 80, 1027 (2005). [2] D. Romanini, M. Chenevrier, S. Kassi, M. Schmidt, C. Valant, M. Ramonet, J. Lopez, and H.-J. Jost, Appl. Phys. B, 83, 659 (2006). [2] E.R.T. Kerstel, R.Q. Iannone, M. Chenevrier, S. Kassi, H.-J. Jost and D. Romanini, Appl. Phys. B, 84, 343 (2006). [4] I. Courtillot, J. Morville, V. Motto-Ros, and D. Romanini, Appl. Phys. B, 85, 407 (2006).

A highly sensitive and selective aptamer-based colorimetric sensor for the rapid detection of PCB 77.

PubMed

Cheng, Ruojie; Liu, Siyao; Shi, Huijie; Zhao, Guohua

2018-01-05

A highly sensitive, specific and simple colorimetric sensor based on aptamer was established for the detection of polychlorinated biphenyls (PCB 77). The use of unmodified gold nanoparticles as a colorimetric probe for aptamer sensors enabled the highly sensitive and selective detection of polychlorinated biphenyls (PCB 77). A linear range of 0.5nM to 900nM was obtained for the colorimetric assay with a minimum detection limit of 0.05nM. In addition, by the methods of circular dichroism, UV and naked eyes, we found that the 35 base fragments retained after cutting 5 bases from the 5 'end of aptamer plays the most significant role in the PCB 77 specific recognition process. We found a novel way to truncated nucleotides to optimize the detection of PCB 77, and the selected nucleotides also could achieve high affinity with PCB 77. At the same time, the efficient detection of the PCB 77 by our colorimetric sensor in the complex environmental water samples was realized, which shows a good application prospect. Copyright © 2017 Elsevier B.V. All rights reserved.

Simultaneous atmospheric nitrous oxide, methane and water vapor detection with a single continuous wave quantum cascade laser.

PubMed

Cao, Yingchun; Sanchez, Nancy P; Jiang, Wenzhe; Griffin, Robert J; Xie, Feng; Hughes, Lawrence C; Zah, Chung-en; Tittel, Frank K

2015-02-09

A continuous wave (CW) quantum cascade laser (QCL) based absorption sensor system was demonstrated and developed for simultaneous detection of atmospheric nitrous oxide (N(2)O), methane (CH(4)), and water vapor (H(2)O). A 7.73-µm CW QCL with its wavelength scanned over a spectral range of 1296.9-1297.6 cm(-1) was used to simultaneously target three neighboring strong absorption lines, N(2)O at 1297.05 cm(-1), CH(4) at 1297.486 cm(-1), and H(2)O at 1297.184 cm(-1). An astigmatic multipass Herriott cell with a 76-m path length was utilized for laser based gas absorption spectroscopy at an optimum pressure of 100 Torr. Wavelength modulation and second harmonic detection was employed for data processing. Minimum detection limits (MDLs) of 1.7 ppb for N(2)O, 8.5 ppb for CH(4), and 11 ppm for H(2)O were achieved with a 2-s integration time for individual gas detection. This single QCL based multi-gas detection system possesses applications in environmental monitoring and breath analysis.

High-resolution all-optical photoacoustic imaging system for remote interrogation of biological specimens

NASA Astrophysics Data System (ADS)

Sampathkumar, Ashwin

2014-05-01

Conventional photoacoustic imaging (PAI) employs light pulses to produce a photoacoustic (PA) effect and detects the resulting acoustic waves using an ultrasound transducer acoustically coupled to the target tissue. The resolution of conventional PAI is limited by the sensitivity and bandwidth of the ultrasound transducer. We have developed an all-optical versatile PAI system for characterizing ex vivo and in vivo biological specimens. The system employs noncontact interferometric detection of the acoustic signals that overcomes limitations of conventional PAI. A 532-nm pump laser with a pulse duration of 5 ns excited the PA effect in tissue. Resulting acoustic waves produced surface displacements that were sensed using a 532-nm continuous-wave (CW) probe laser in a Michelson interferometer with a GHz bandwidth. The pump and probe beams were coaxially focused using a 50X objective giving a diffraction-limited spot size of 0.48 μm. The phase-encoded probe beam was demodulated using a homodyne interferometer. The detected time-domain signal was time reversed using k-space wave-propagation methods to produce a spatial distribution of PA sources in the target tissue. Performance was assessed using PA images of ex vivo rabbit lymph node specimens and human tooth samples. A minimum peak surface displacement sensitivity of 0.19 pm was measured. The all-optical PAI (AOPAI) system is well suited for assessment of retinal diseases, caries lesion detection, skin burns, section less histology and pressure or friction ulcers.

DIF Detection Using Multiple-Group Categorical CFA with Minimum Free Baseline Approach

ERIC Educational Resources Information Center

Chang, Yu-Wei; Huang, Wei-Kang; Tsai, Rung-Ching

2015-01-01

The aim of this study is to assess the efficiency of using the multiple-group categorical confirmatory factor analysis (MCCFA) and the robust chi-square difference test in differential item functioning (DIF) detection for polytomous items under the minimum free baseline strategy. While testing for DIF items, despite the strong assumption that all…

Support vector machine as a binary classifier for automated object detection in remotely sensed data

NASA Astrophysics Data System (ADS)

Wardaya, P. D.

2014-02-01

In the present paper, author proposes the application of Support Vector Machine (SVM) for the analysis of satellite imagery. One of the advantages of SVM is that, with limited training data, it may generate comparable or even better results than the other methods. The SVM algorithm is used for automated object detection and characterization. Specifically, the SVM is applied in its basic nature as a binary classifier where it classifies two classes namely, object and background. The algorithm aims at effectively detecting an object from its background with the minimum training data. The synthetic image containing noises is used for algorithm testing. Furthermore, it is implemented to perform remote sensing image analysis such as identification of Island vegetation, water body, and oil spill from the satellite imagery. It is indicated that SVM provides the fast and accurate analysis with the acceptable result.

Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin

PubMed Central

Ismail, Fatma; Adeloju, Samuel B.

2010-01-01

A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3, and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 μM for penicillin and achieved a minimum detectable concentration of 0.3 μM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm. PMID:22319276

Acousto-Optic Q-Switched Fiber Laser-Based Intra-Cavity Photoacoustic Spectroscopy for Trace Gas Detection

PubMed Central

Zhang, Qinduan; Chang, Jun; Wang, Zongliang; Wang, Fupeng; Qin, Zengguang

2017-01-01

We proposed a new method for gas detection in photoacoustic spectroscopy based on acousto-optic Q-switched fiber laser by merging a transmission PAS cell (resonant frequency f0 = 5.3 kHz) inside the fiber laser cavity. The Q-switching was achieved by an acousto-optic modulator, achieving a peak pulse power of ~679 mW in the case of the acousto-optic modulation signal with an optimized duty ratio of 10%. We used a custom-made fiber Bragg grating with a central wavelength of 1530.37 nm (the absorption peak of C2H2) to select the laser wavelength. The system achieved a linear response (R2 = 0.9941) in a concentration range from 400 to 7000 ppmv, and the minimum detection limit compared to that of a conventional intensity modulation system was enhanced by 94.2 times. PMID:29295599

High-performance electrochemical glucose sensing enabled by Cu(TCNQ) nanorod array

NASA Astrophysics Data System (ADS)

Wu, Xiufeng; Lu, Wenbo

2018-04-01

It is highly attractive to construct stable enzyme-free glucose sensors based on three-dimensional direct electrochemical detection of glucose. In this paper, a copper 7,7,8,8-tetracyanoquinodimethane (Cu(TCNQ)) nanorod array on Cu foam (Cu(TCNQ) NA/CF) is proposed as an efficient catalyst for electrochemical glucose oxidation in alkaline conditions. When Cu(TCNQ) NA/CF was used as the enzyme-free sensory of glucose, the sensor showed a response time within 3 s, a wide linear detection in the range 0.001-10.0 mM, the minimum limit of detection was as low as 10 nM (S/N = 3), and it had a high sensitivity of 26 987 μA mM-1 cm-2. Moreover, this sensor also possesses long-term stability, high selectivity, reproducibility, and actual applications for fresh human serum sample analysis is also successfully accepted.

Highly Sensitive and Multifunctional Tactile Sensor Using Free-standing ZnO/PVDF Thin Film with Graphene Electrodes for Pressure and Temperature Monitoring

PubMed Central

Lee, James S.; Shin, Keun-Young; Cheong, Oug Jae; Kim, Jae Hyun; Jang, Jyongsik

2015-01-01

We demonstrate an 80-μm-thick film (which is around 15% of the thickness of the human epidermis), which is a highly sensitive hybrid functional gauge sensor, and was fabricated from poly(vinylidene fluoride) (PVDF) and ZnO nanostructures with graphene electrodes. Using this film, we were able to simultaneously measure pressure and temperature in real time. The pressure was monitored from the change in the electrical resistance via the piezoresistance of the material, and the temperature was inferred based on the recovery time of the signal. Our thin film system enabled us to detect changes in pressure as small as 10 Pa which is pressure detection limit was 103-fold lower than the minimum level required for artificial skin, and to detect temperatures in the range 20–120°C. PMID:25601479

Comparison of randomly cloned and whole genomic DNA probes for the detection of Porphyromonas gingivalis and Bacteroides forsythus

PubMed Central

Wong, M.; DiRienzo, J.M.; Lai, C.-H.; Listgarten, M. A.

2012-01-01

Whole genomic and randomly-cloned DNA probes for two fastidious periodontal pathogens, Porphyromonas gingivalis and Bacteroides forsythus were labeled with digoxigenin and detected by a colorimetric method. The specificity and sensitivity of the whole genomic and cloned probes were compared. The cloned probes were highly specific compared to the whole genomic probes. A significant degree of cross-reactivity with Bacteroides species. Capnocytophaga sp. and Prevotella sp. was observed with the whole genomic probes. The cloned probes were less sensitive than the whole genomic probes and required at least 106 target cells or a minimum of 10 ng of target DNA to be detected during hybridization. Although a ten-fold increase in sensitivity was obtained with the whole genomic probes, cross-hybridization to closely related species limits their reliability in identifying target bacteria in subgingival plaque samples. PMID:8636873

Dual quantum cascade laser-based sensor for simultaneous NO and NO2 detection using a wavelength modulation-division multiplexing technique

NASA Astrophysics Data System (ADS)

Yu, Yajun; Sanchez, Nancy P.; Yi, Fan; Zheng, Chuantao; Ye, Weilin; Wu, Hongpeng; Griffin, Robert J.; Tittel, Frank K.

2017-05-01

A sensor system capable of simultaneous measurements of NO and NO2 was developed using a wavelength modulation-division multiplexing (WMDM) scheme and multi-pass absorption spectroscopy. A continuous wave (CW), distributed-feedback (DFB) quantum cascade laser (QCL) and a CW external-cavity (EC) QCL were employed for targeting a NO absorption doublet at 1900.075 cm-1 and a NO2 absorption line at 1630.33 cm-1, respectively. Simultaneous detection was realized by modulating both QCLs independently at different frequencies and demodulating the detector signals with LabView-programmed lock-in amplifiers. The sensor operated at a reduced pressure of 40 Torr and a data sampling rate of 1 Hz. An Allan-Werle deviation analysis indicated that the minimum detection limits of NO and NO2 can reach sub-ppbv concentration levels with averaging times of 100 and 200 s, respectively.

Highly sensitive and multifunctional tactile sensor using free-standing ZnO/PVDF thin film with graphene electrodes for pressure and temperature monitoring.

PubMed

Lee, James S; Shin, Keun-Young; Cheong, Oug Jae; Kim, Jae Hyun; Jang, Jyongsik

2015-01-20

We demonstrate an 80-μm-thick film (which is around 15% of the thickness of the human epidermis), which is a highly sensitive hybrid functional gauge sensor, and was fabricated from poly(vinylidene fluoride) (PVDF) and ZnO nanostructures with graphene electrodes. Using this film, we were able to simultaneously measure pressure and temperature in real time. The pressure was monitored from the change in the electrical resistance via the piezoresistance of the material, and the temperature was inferred based on the recovery time of the signal. Our thin film system enabled us to detect changes in pressure as small as 10 Pa which is pressure detection limit was 10(3)-fold lower than the minimum level required for artificial skin, and to detect temperatures in the range 20-120 °C.

Polarimetric Analysis of the Long Duration Gamma-Ray Burst GRB 160530A With the Balloon Borne Compton Spectrometer and Imager

NASA Astrophysics Data System (ADS)

Lowell, A. W.; Boggs, S. E.; Chiu, C. L.; Kierans, C. A.; Sleator, C.; Tomsick, J. A.; Zoglauer, A. C.; Chang, H.-K.; Tseng, C.-H.; Yang, C.-Y.; Jean, P.; von Ballmoos, P.; Lin, C.-H.; Amman, M.

2017-10-01

A long duration gamma-ray burst, GRB 160530A, was detected by the Compton Spectrometer and Imager (COSI) during the 2016 COSI Super Pressure Balloon campaign. As a Compton telescope, COSI is inherently sensitive to the polarization of gamma-ray sources in the energy range 0.2-5.0 MeV. We measured the polarization of GRB 160530A using (1) a standard method (SM) based on fitting the distribution of azimuthal scattering angles with a modulation curve and (2) an unbinned, maximum likelihood method (MLM). In both cases, the measured polarization level was below the 99% confidence minimum detectable polarization levels of 72.3% ± 0.8% (SM) and 57.5% ± 0.8% (MLM). Therefore, COSI did not detect polarized gamma-ray emission from this burst. Our most constraining 90% confidence upper limit on the polarization level was 46% (MLM).

Acousto-Optic Q-Switched Fiber Laser-Based Intra-Cavity Photoacoustic Spectroscopy for Trace Gas Detection.

PubMed

Zhang, Qinduan; Chang, Jun; Wang, Qiang; Wang, Zongliang; Wang, Fupeng; Qin, Zengguang

2017-12-25

We proposed a new method for gas detection in photoacoustic spectroscopy based on acousto-optic Q-switched fiber laser by merging a transmission PAS cell (resonant frequency f ₀ = 5.3 kHz) inside the fiber laser cavity. The Q-switching was achieved by an acousto-optic modulator, achieving a peak pulse power of ~679 mW in the case of the acousto-optic modulation signal with an optimized duty ratio of 10%. We used a custom-made fiber Bragg grating with a central wavelength of 1530.37 nm (the absorption peak of C₂H₂) to select the laser wavelength. The system achieved a linear response (R² = 0.9941) in a concentration range from 400 to 7000 ppmv, and the minimum detection limit compared to that of a conventional intensity modulation system was enhanced by 94.2 times.

Intracavity Faraday modulation spectroscopy (INFAMOS): A tool for radical detection.

PubMed

Gianella, Michele; Pinto, Tomas H P; Wu, Xia; Ritchie, Grant A D

2017-08-07

We present the intra-cavity Faraday modulation spectroscopy technique, whereby optical feedback cavity-enhanced spectroscopy is coupled with Faraday modulation spectroscopy to greatly enhance the interaction path length of a laser beam with a paramagnetic sample in a magnetic field. We describe a first prototype based upon a cw quantum cascade laser targeting a selection of fundamental rovibrational R-branch transitions of nitric oxide (1890 cm -1 ), consisting of a linear cavity (finesse F=6300) and a water-cooled solenoid. We demonstrate a minimum detectable Verdet constant of V min =4.7×10 -14  rad cm -1  G -1  Hz -1/2 (at SNR = 1), corresponding to a single-pass rotation angle of 1.6×10 -10  rad Hz -1/2 and a limit of detection of 0.21 ppbv Hz -1/2 NO.

Development and evaluation of an off-the-slide genotyping technique for identifying Giardia cysts and Cryptosporidium oocysts directly from US EPA Method 1623 slides.

PubMed

Ware, M W; Keely, S P; Villegas, E N

2013-07-01

This study developed and systematically evaluated performance and limit of detection of an off-the-slide genotyping procedure for both Cryptosporidium oocysts and Giardia cysts. Slide standards containing flow-sorted (oo)cysts were used to evaluate the off-the-slide genotyping procedure by microscopy and PCR. Results show approximately 20% of cysts and oocysts are lost during staining. Although transfer efficiency from the slide to the PCR tube could not be determined by microscopy, it was observed that the transfer process aided in the physical lysis of the (oo)cysts likely releasing DNA. PCR detection rates for a single event on a slide were 44% for Giardia and 27% for Cryptosporidium, and a minimum of five cysts and 20 oocysts are required to achieve a 90% PCR detection rate. A Poisson distribution analysis estimated the relative PCR target densities and limits of detection, it showed that 18 Cryptosporidium and five Giardia replicates are required for a 95% probability of detecting a single (oo)cyst on a slide. This study successfully developed and evaluated recovery rates and limits of detection of an off-the-slide genotyping procedure for both Cryptosporidium and Giardia (oo)cysts from the same slide. This off-the-slide genotyping technique is a simple and low cost tool that expands the applications of US EPA Method 1623 results by identifying the genotypes and assemblages of the enumerated Cryptosporidium and Giardia. This additional information will be useful for microbial risk assessment models and watershed management decisions. Journal of Applied Microbiology Published [2013]. This article is a U.S. Government work and is in the public domain in the USA.

High-Field Quench Behavior and Protection of $$Bi_2 Sr_2 Ca Cu_2 O_x$$ Coils: Minimum and Maximum Quench Detection Voltages

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shen, Tengming; Ye, Liyang; Turrioni, Daniele

Small insert coils have been built using a multifilamentary Bi2Sr2CaCu2Ox round wire, and characterized in background fields to explore the quench behaviors and limits of Bi2Sr2CaCu2Ox superconducting magnets, with an emphasis on assessing the impact of slow normal zone propagation on quench detection. Using heaters of various lengths to initiate a small normal zone, a coil was quenched safely more than 70 times without degradation, with the maximum coil temperature reaching 280 K. Coils withstood a resistive voltage of tens of mV for seconds without quenching, showing the high stability of these coils and suggesting that the quench detection voltagemore » shall be greater than 50 mV to not to falsely trigger protection. The hot spot temperature for the resistive voltage of the normal zone to reach 100 mV increases from ~40 K to ~80 K with increasing the operating wire current density Jo from 89 A/mm2 to 354 A/mm2 whereas for the voltage to reach 1 V, it increases from ~60 K to ~140 K, showing the increasing negative impact of slow normal zone propagation on quench detection with increasing Jo and the need to limit the quench detection voltage to < 1 V. These measurements, coupled with an analytical quench model, were used to access the impact of the maximum allowable voltage and temperature upon quench detection on the quench protection, assuming to limit the hot spot temperature to <300 K.« less

A Validation of Remotely Sensed Fires Using Ground Reports

NASA Astrophysics Data System (ADS)

Ruminski, M. G.; Hanna, J.

2007-12-01

A satellite based analysis of fire detections and smoke emissions for North America is produced daily by NOAA/NESDIS. The analysis incorporates data from the MODIS (Terra and Aqua) and AVHRR (NOAA-15/16/17) polar orbiting instruments and GOES East and West geostationary spacecraft with nominal resolutions of 1km and 4 km for the polar and geostationary platforms respectively. Automated fire detection algorithms are utilized for each of the sensors. Analysts perform a quality control procedure on the automated detects by deleting points that are deemed to be false detects and adding points that the algorithms did not detect. A limited validation of the final quality controlled product was performed using high resolution (30 m) ASTER data in the summer of 2006. Some limitations in using ASTER data are that each scene is only approximately 3600 square km, the data acquisition time is relatively constant at around 1030 local solar time and ASTER is another remotely sensed data source. This study expands on the ASTER validation by using ground reports of prescribed burns in Montana and Idaho for 2003 and 2004. It provides a non-remote sensing data source for comparison. While the ground data do not have the limitations noted above for ASTER there are still limitations. For example, even though the data set covers a much larger area (nearly 600,000 square km) than even several ASTER scenes, it still represents a single region of North America. And while the ground data are not restricted to a narrow time window, only a date is provided with each report, limiting the ability to make detailed conclusions about the detection capabilities for specific instruments, especially for the less temporally frequent polar orbiting MODIS and AVHRR sensors. Comparison of the ground data reports to the quality controlled fire analysis revealed a low rate of overall detection of 23.00% over the entire study period. Examination of the daily detection rates revealed a wide variation, with some days resulting in as little as 5 detects out of 107 reported fires while other days had as many as 84 detections out of 160 reports. Inspection of the satellite imagery from the days with very low detection rates revealed that extensive cloud cover prohibited satellite fire detection. On days when cloud cover was at a minimum, detection rates were substantially higher. An estimate of the fire size was also provided with the ground data set. Statistics will be presented for days with minimal cloud cover which will indicate the probability of detection for fires of various sizes.

[Long-term outcome of vitrectomy combined with internal limiting membrane peeling for idiopathic macular holes].

PubMed

Yamamoto, Kaori; Hori, Sadao

2011-01-01

To elucidate the long-term outcome of internal limiting membrane (ILM) peeling on visual function during vitrectomy for idiopathic macular holes using scanning laser ophthalmoscope (SLO) microperimetry. Prospective uncontrolled study. We studied 31 eyes (29 patients) with idiopathic macular holes. All patients underwent vitrectomy with ILM peeling. The SLO microperimetry was performed preoperatively, and once a year for 3 years postoperatively to detect scotomas in and around the macular holes, and both within and in close vicinity to the areas of ILM peeling. Closure of macular holes after one surgery was confirmed in all cases except for 2 with second surgery. The visual acuity by logarithmic minimum angle of resolution (logMAR) averaged 0.71 +/- 0.36 before surgery, 0.23 +/- 0.31 one year, 0.14 +/- 0.27 two years and 0.12 +/- 0.26 three years after surgery. There was significant improvement up to 2 years after the surgery. All scotomas detected before surgery in the holes, and 77.4% of those detected around the holes decreased gradually. No scotomas were detected in or around the area of ILM peeling either before or after surgery. ILM peeling in vitrectomy for idiopathic macular holes successfully improved visual acuity and did not influence retinal sensitivity in and around the area of ILM peeling. The scotomas detected in and around the holes before surgery gradually reduced or disappeared.

Soil carbon inventories under a bioenergy crop (switchgrass): Measurement limitations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garten, C.T. Jr.; Wullschleger, S.D.

Approximately 5 yr after planting, coarse root carbon (C) and soil organic C (SOC) inventories were compared under different types of plant cover at four switchgrass (Panicum virgatum L.) production field trials in the southeastern USA. There was significantly more coarse root C under switchgrass (Alamo variety) and forest cover than tall fescue (Festuca arundinacea Schreb.), corn (Zea mays L.), or native pastures of mixed grasses. Inventories of SOC under switchgrass were not significantly greater than SOC inventories under other plant covers. At some locations the statistical power associated with ANOVA of SOC inventories was low, which raised questions aboutmore » whether differences in SOC could be detected statistically. A minimum detectable difference (MDD) for SOC inventories was calculated. The MDD is the smallest detectable difference between treatment means once the variation, significance level, statistical power, and sample size are specified. The analysis indicated that a difference of {approx}50 mg SOC/cm{sup 2} or 5 Mg SOC/ha, which is {approx}10 to 15% of existing SOC, could be detected with reasonable sample sizes and good statistical power. The smallest difference in SOC inventories that can be detected, and only with exceedingly large sample sizes, is {approx}2 to 3%. These measurement limitations have implications for monitoring and verification of proposals to ameliorate increasing global atmospheric CO{sub 2} concentrations by sequestering C in soils.« less

Experimental demonstration of all-optical weak magnetic field detection using beam-deflection of single-mode fiber coated with cobalt-doped nickel ferrite nanoparticles.

PubMed

Pradhan, Somarpita; Chaudhuri, Partha Roy

2015-07-10

We experimentally demonstrate single-mode optical-fiber-beam-deflection configuration for weak magnetic-field-detection using an optimized (low coercive-field) composition of cobalt-doped nickel ferrite nanoparticles. Devising a fiber-double-slit type experiment, we measure the surrounding magnetic field through precisely measuring interference-fringe yielding a minimum detectable field ∼100  mT and we procure magnetization data of the sample that fairly predicts SQUID measurement. To improve sensitivity, we incorporate etched single-mode fiber in double-slit arrangement and recorded a minimum detectable field, ∼30  mT. To further improve, we redefine the experiment as modulating fiber-to-fiber light-transmission and demonstrate the minimum field as 2.0 mT. The device will be uniquely suited for electrical or otherwise hazardous environments.

Caracterización de un sistema de telescopios Cherenkov para la detección de rayos gamma de energías del TeV desde el CASLEO

NASA Astrophysics Data System (ADS)

Melo, D.; Yelós, L. D.; Garcia, B.; Rovero, A. C.

2017-10-01

Gamma-ray astronomy opened the universe of the more energetic electromagnetic radiation using ground and orbiting instruments, which provide information for the understanding of sources of different types. Ground-based telescope arrays use Cherenkov light produced by the charged particles from extensive air showers generated in the Earth's atmosphere to identify gamma rays. This imposes a minimum energy threshold on the gamma rays to be detected. Towards the high-energy end of the spectrum, however, the amount of Cherenkov radiation produced by a gamma-ray photon guarantees its detectability, the limiting factor being the low flux of the sources. For this reason, the detection strategy consists in using arrays of small telescopes. In this work, we investigate the feasibility of detecting gamma-ray cascades using Cherenkov telescopes, in the range of 100 GeV to 2 TeV, at the CASLEO site, characterizing the response of a system of three Cherenkov telescopes.

Effect of censoring trace-level water-quality data on trend-detection capability

USGS Publications Warehouse

Gilliom, R.J.; Hirsch, R.M.; Gilroy, E.J.

1984-01-01

Monte Carlo experiments were used to evaluate whether trace-level water-quality data that are routinely censored (not reported) contain valuable information for trend detection. Measurements are commonly censored if they fall below a level associated with some minimum acceptable level of reliability (detection limit). Trace-level organic data were simulated with best- and worst-case estimates of measurement uncertainty, various concentrations and degrees of linear trend, and different censoring rules. The resulting classes of data were subjected to a nonparametric statistical test for trend. For all classes of data evaluated, trends were most effectively detected in uncensored data as compared to censored data even when the data censored were highly unreliable. Thus, censoring data at any concentration level may eliminate valuable information. Whether or not valuable information for trend analysis is, in fact, eliminated by censoring of actual rather than simulated data depends on whether the analytical process is in statistical control and bias is predictable for a particular type of chemical analyses.

Acetylene measurement in flames by chirp-based quantum cascade laser spectrometry.

PubMed

Quine, Zachary R; McNesby, Kevin L

2009-06-01

We have designed and characterized a mid-IR spectrometer built around a pulsed distributed-feedback quantum cascade laser using the characteristic frequency down-chirp to scan through the spectral region 6.5 cm(-1) spectral region. The behavior of this chirp is extensively measured. The accuracy and detection limits of the system as an absorption spectrometer are demonstrated first by measuring spectra of acetylene through a single pass 16 cm absorption cell in real time at low concentrations and atmospheric pressure. The smallest detectable peak is measured to be approximately 1.5 x 10(-4) absorbance units, yielding a minimum detectable concentration length product of 2.4 parts per million meter at standard temperature and pressure. This system is then used to detect acetylene within an ethylene-air opposed flow flame. Measurements of acetylene content as a function of height above the fuel source are presented, as well as measurements of acetylene produced in fuel breakdown as a function of preinjection fuel temperature.

Optimization of data analysis for the in vivo neutron activation analysis of aluminum in bone.

PubMed

Mohseni, H K; Matysiak, W; Chettle, D R; Byun, S H; Priest, N; Atanackovic, J; Prestwich, W V

2016-10-01

An existing system at McMaster University has been used for the in vivo measurement of aluminum in human bone. Precise and detailed analysis approaches are necessary to determine the aluminum concentration because of the low levels of aluminum found in the bone and the challenges associated with its detection. Phantoms resembling the composition of the human hand with varying concentrations of aluminum were made for testing the system prior to the application to human studies. A spectral decomposition model and a photopeak fitting model involving the inverse-variance weighted mean and a time-dependent analysis were explored to analyze the results and determine the model with the best performance and lowest minimum detection limit. The results showed that the spectral decomposition and the photopeak fitting model with the inverse-variance weighted mean both provided better results compared to the other methods tested. The spectral decomposition method resulted in a marginally lower detection limit (5μg Al/g Ca) compared to the inverse-variance weighted mean (5.2μg Al/g Ca), rendering both equally applicable to human measurements. Copyright © 2016 Elsevier Ltd. All rights reserved.

Sensitive detection of chemical agents and toxic industrial chemicals using active open-path FTIRs

NASA Astrophysics Data System (ADS)

Walter, William T.

2004-03-01

Active open-path FTIR sensors provide more sensitive detection of chemical agents than passive FTIRs, such as the M21 RSCAAL and JSLSCAD, and at the same time identify and quantify toxic industrial chemicals (TIC). Passive FTIRs are bistatic sensors relying on infrared sources of opportunity. Utilization of earth-based sources of opportunity limits the source temperatures available for passive chemical-agent FTIR sensors to 300° K. Active FTIR chemical-agent sensors utilize silicon carbide sources, which can be operated at 1500° K. The higher source temperature provides more than an 80-times increase in the infrared radiant flux emitted per unit area in the 7 to 14 micron spectral fingerprint region. Minimum detection limits are better than 5 μgm/m3 for GA, GB, GD, GF and VX. Active FTIR sensors can (1) assist first responders and emergency response teams in their assessment of and reaction to a terrorist threat, (2) provide information on the identification of the TIC present and their concentrations and (3) contribute to the understanding and prevention of debilitating disorders analogous to the Gulf War Syndrome for military and civilian personnel.

The combination design for open and endoscopic surgery using fluorescence molecular imaging technology

NASA Astrophysics Data System (ADS)

Mao, Yamin; Jiang, Shixin; Ye, Jinzuo; An, Yu; Yang, Xin; Chi, Chongwei; Tian, Jie

2015-03-01

For clinical surgery, it is still a challenge to objectively determine tumor margins during surgery. With the development of medical imaging technology, fluorescence molecular imaging (FMI) method can provide real-time intraoperative tumor margin information. Furthermore, surgical navigation system based on FMI technology plays an important role for the aid of surgeons' precise tumor margin decision. However, detection depth is the most limitation exists in the FMI technique and the method convenient for either macro superficial detection or micro deep tissue detection is needed. In this study, we combined advantages of both open surgery and endoscopic imaging systems with FMI technology. Indocyanine green (ICG) experiments were performed to confirm the feasibility of fluorescence detection in our system. Then, the ICG signal was photographed in the detection area with our system. When the system connected with endoscope lens, the minimum quantity of ICG detected by our system was 0.195 ug. For aspect of C mount lens, the sensitivity of ICG detection with our system was 0.195ug. Our experiments results proved that it was feasible to detect fluorescence images with this combination method. Our system shows great potential in the clinical applications of precise dissection of various tumors

49 CFR Appendix A to Part 172 - Office of Hazardous Materials Transportation Color Tolerance Charts and Tables

Code of Federal Regulations, 2010 CFR

2010-10-01

... .3308 .2328 Dark 10.0P 4.0/10 12.00 .3306 .2162 1 Maximum chroma is not limited. 2 For the colors green and purple, the minimum saturation (chroma) limits for porcelain enamel on metal are lower than for most other surface coatings. Therefore, the minimum chroma limits of these two colors as displayed on...

40 CFR Table 11 to Subpart Xxxx of... - Minimum Data for Continuous Compliance With the Emission Limits for Tire Cord Production Affected...

Code of Federal Regulations, 2010 CFR

2010-07-01

... With the Emission Limits for Tire Cord Production Affected Sources 11 Table 11 to Subpart XXXX of Part... for Hazardous Air Pollutants: Rubber Tire Manufacturing Pt. 63, Subpt. XXXX, Table 11 Table 11 to Subpart XXXX of Part 63—Minimum Data for Continuous Compliance With the Emission Limits for Tire Cord...

Determination of the Minimum Use Level of Fuel System Icing Inhibitor (FSII) in JP-8 That Will Provide Adquate Icing Inhibition and Biostatic Protection for Air Force Aircraft

DTIC Science & Technology

2013-12-01

experimental studies and analyses performed and the resulting recommendations. Results from the present effort indicated that a minimum use limit of... experimental studies performed and the resulting recommendations regarding the minimum on-board use limit of FSII while maintaining safe operability...sumping. A detailed summary of the experimental efforts and results are provided in a separate report (Balster et al., 2010). For the ATCC

Backscatter particle image velocimetry via optical time-of-flight sectioning

DOE PAGES

Paciaroni, Megan E.; Chen, Yi; Lynch, Kyle Patrick; ...

2018-01-11

Conventional particle image velocimetry (PIV) configurations require a minimum of two optical access ports, inherently restricting the technique to a limited class of flows. Here, the development and application of a novel method of backscattered time-gated PIV requiring a single-optical-access port is described along with preliminary results. The light backscattered from a seeded flow is imaged over a narrow optical depth selected by an optical Kerr effect (OKE) time gate. The picosecond duration of the OKE time gate essentially replicates the width of the laser sheet of conventional PIV by limiting detected photons to a narrow time-of-flight within the flow.more » Thus, scattering noise from outside the measurement volume is eliminated. In conclusion, this PIV via the optical time-of-flight sectioning technique can be useful in systems with limited optical access and in flows near walls or other scattering surfaces.« less

Backscatter particle image velocimetry via optical time-of-flight sectioning

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paciaroni, Megan E.; Chen, Yi; Lynch, Kyle Patrick

Conventional particle image velocimetry (PIV) configurations require a minimum of two optical access ports, inherently restricting the technique to a limited class of flows. Here, the development and application of a novel method of backscattered time-gated PIV requiring a single-optical-access port is described along with preliminary results. The light backscattered from a seeded flow is imaged over a narrow optical depth selected by an optical Kerr effect (OKE) time gate. The picosecond duration of the OKE time gate essentially replicates the width of the laser sheet of conventional PIV by limiting detected photons to a narrow time-of-flight within the flow.more » Thus, scattering noise from outside the measurement volume is eliminated. In conclusion, this PIV via the optical time-of-flight sectioning technique can be useful in systems with limited optical access and in flows near walls or other scattering surfaces.« less

Cloud-to-Ground Lightning Estimates Derived from SSMI Microwave Remote Sensing and NLDN

NASA Technical Reports Server (NTRS)

Winesett, Thomas; Magi, Brian; Cecil, Daniel

2015-01-01

Lightning observations are collected using ground-based and satellite-based sensors. The National Lightning Detection Network (NLDN) in the United States uses multiple ground sensors to triangulate the electromagnetic signals created when lightning strikes the Earth's surface. Satellite-based lightning observations have been made from 1998 to present using the Lightning Imaging Sensor (LIS) on the NASA Tropical Rainfall Measuring Mission (TRMM) satellite, and from 1995 to 2000 using the Optical Transient Detector (OTD) on the Microlab-1 satellite. Both LIS and OTD are staring imagers that detect lightning as momentary changes in an optical scene. Passive microwave remote sensing (85 and 37 GHz brightness temperatures) from the TRMM Microwave Imager (TMI) has also been used to quantify characteristics of thunderstorms related to lightning. Each lightning detection system has fundamental limitations. TRMM satellite coverage is limited to the tropics and subtropics between 38 deg N and 38 deg S, so lightning at the higher latitudes of the northern and southern hemispheres is not observed. The detection efficiency of NLDN sensors exceeds 95%, but the sensors are only located in the USA. Even if data from other ground-based lightning sensors (World Wide Lightning Location Network, the European Cooperation for Lightning Detection, and Canadian Lightning Detection Network) were combined with TRMM and NLDN, there would be enormous spatial gaps in present-day coverage of lightning. In addition, a globally-complete time history of observed lightning activity is currently not available either, with network coverage and detection efficiencies varying through the years. Previous research using the TRMM LIS and Microwave Imager (TMI) showed that there is a statistically significant correlation between lightning flash rates and passive microwave brightness temperatures. The physical basis for this correlation emerges because lightning in a thunderstorm occurs where ice is first present in the cloud and electric charge separation occurs. These ice particles efficiently scatter the microwave radiation at the 85 and 37 GHz frequencies, thus leading to large brightness temperature depressions. Lightning flash rate is related to the total amount of ice passing through the convective updraft regions of thunderstorms. Confirmation of this relationship using TRMM LIS and TMI data, however, remains constrained to TRMM observational limits of the tropics and subtropics. Satellites from the Defense Meteorology Satellite Program (DMSP) have global coverage and are equipped with passive microwave imagers that, like TMI, observe brightness temperatures at 85 and 37 GHz. Unlike the TRMM satellite, however, DMSP satellites do not have a lightning sensor, and the DMSP microwave data has never been used to derive global lightning. In this presentation, a relationship between DMSP Special Sensor Microwave Imager (SSMI) data and ground-based cloud-to-ground (CG) lightning data from NLDN is investigated to derive a spatially complete time history of CG lightning for the USA study area. This relationship is analogous to the established using TRMM LIS and TMI data. NLDN has the most spatially and temporally complete CG lightning data for the USA, and therefore provides the best opportunity to find geospatially coincident observations with SSMI sensors. The strongest thunderstorms generally have minimum 85 GHz Polarized Corrected brightness Temperatures (PCT) less than 150 K. Archived radar data was used to resolve the spatial extent of the individual storms. NLDN data for that storm spatial extent defined by radar data was used to calculate the CG flash rate for the storm. Similar to results using TRMM sensors, a linear model best explained the relationship between storm-specific CG flash rates and minimum 85 GHz PCT. However, the results in this study apply only to CG lightning. To extend the results to weaker storms, the probability of CG lightning (instead of the flash rate) was calculated for storms having 85 GHz PCT greater than 150 K. NLDN data was used to determine if a CG strike occurred for a storm. This probability of CG lightning was plotted as a function of minimum 85 GHz PCT and minimum 37 GHz PCT. These probabilities were used in conjunction with the linear model to estimate the CG flash rate for weaker storms with minimum 85 GHz PCTs greater than 150 K. Results from the investigation of CG lightning and passive microwave radiation signals agree with the previous research investigating total lightning and brightness temperature. Future work will take the established relationships and apply them to the decades of available DMSP data for the USA to derive a map of CG lightning flash rates. Validation of this method and uncertainty analysis will be done by comparing the derived maps of CG lightning flash rates against existing NLDN maps of CG lightning flash rates.

Development and validation of an automated unit for the extraction of radiocaesium from seawater.

PubMed

Bokor, Ilonka; Sdraulig, Sandra; Jenkinson, Peter; Madamperuma, Janaka; Martin, Paul

2016-01-01

An automated unit was developed for the in-situ extraction of radiocaesium ((137)Cs and (134)Cs) from large volumes of seawater to achieve very low detection limits. The unit was designed for monitoring of Australian ocean and coastal waters, including at ports visited by nuclear-powered warships. The unit is housed within a robust case, and is easily transported and operated. It contains four filter cartridges connected in series. The first two cartridges are used to remove any suspended material that may be present in the seawater, while the last two cartridges are coated with potassium copper hexacyanoferrate for caesium extraction. Once the extraction is completed the coated cartridges are ashed. The ash is transferred to a small petri dish for counting of (137)Cs and (134)Cs by high resolution gamma spectrometry for a minimum of 24 h. The extraction method was validated for the following criteria: selectivity, trueness, precision, linearity, limit of detection and traceability. The validation showed the unit to be fit for purpose with the method capable of achieving low detection limits required for environmental samples. The results for the environmental measurements in Australian seawater correlate well with those reported in the Worldwide Marine Radioactivity Study (WOMARS). The cost of preparation and running the system is low and waste generation is minimal. Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

Day, night and all-weather security surveillance automation synergy from combining two powerful technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morellas, Vassilios; Johnson, Andrew; Johnston, Chris

2006-07-01

Thermal imaging is rightfully a real-world technology proven to bring confidence to daytime, night-time and all weather security surveillance. Automatic image processing intrusion detection algorithms are also a real world technology proven to bring confidence to system surveillance security solutions. Together, day, night and all weather video imagery sensors and automated intrusion detection software systems create the real power to protect early against crime, providing real-time global homeland protection, rather than simply being able to monitor and record activities for post event analysis. These solutions, whether providing automatic security system surveillance at airports (to automatically detect unauthorized aircraft takeoff andmore » landing activities) or at high risk private, public or government facilities (to automatically detect unauthorized people or vehicle intrusion activities) are on the move to provide end users the power to protect people, capital equipment and intellectual property against acts of vandalism and terrorism. As with any technology, infrared sensors and automatic image intrusion detection systems for global homeland security protection have clear technological strengths and limitations compared to other more common day and night vision technologies or more traditional manual man-in-the-loop intrusion detection security systems. This paper addresses these strength and limitation capabilities. False Alarm (FAR) and False Positive Rate (FPR) is an example of some of the key customer system acceptability metrics and Noise Equivalent Temperature Difference (NETD) and Minimum Resolvable Temperature are examples of some of the sensor level performance acceptability metrics. (authors)« less

First NuSTAR Limits on Quiet Sun Hard X-Ray Transient Events

NASA Astrophysics Data System (ADS)

Marsh, Andrew J.; Smith, David M.; Glesener, Lindsay; Hannah, Iain G.; Grefenstette, Brian W.; Caspi, Amir; Krucker, Säm; Hudson, Hugh S.; Madsen, Kristin K.; White, Stephen M.; Kuhar, Matej; Wright, Paul J.; Boggs, Steven E.; Christensen, Finn E.; Craig, William W.; Hailey, Charles J.; Harrison, Fiona A.; Stern, Daniel; Zhang, William W.

2017-11-01

We present the first results of a search for transient hard X-ray (HXR) emission in the quiet solar corona with the Nuclear Spectroscopic Telescope Array (NuSTAR) satellite. While NuSTAR was designed as an astrophysics mission, it can observe the Sun above 2 keV with unprecedented sensitivity due to its pioneering use of focusing optics. NuSTAR first observed quiet-Sun regions on 2014 November 1, although out-of-view active regions contributed a notable amount of background in the form of single-bounce (unfocused) X-rays. We conducted a search for quiet-Sun transient brightenings on timescales of 100 s and set upper limits on emission in two energy bands. We set 2.5-4 keV limits on brightenings with timescales of 100 s, expressed as the temperature T and emission measure EM of a thermal plasma. We also set 10-20 keV limits on brightenings with timescales of 30, 60, and 100 s, expressed as model-independent photon fluxes. The limits in both bands are well below previous HXR microflare detections, though not low enough to detect events of equivalent T and EM as quiet-Sun brightenings seen in soft X-ray observations. We expect future observations during solar minimum to increase the NuSTAR sensitivity by over two orders of magnitude due to higher instrument livetime and reduced solar background.

50 CFR 622.50 - Caribbean spiny lobster import prohibitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Caribbean spiny lobster import... ATLANTIC Management Measures § 622.50 Caribbean spiny lobster import prohibitions. (a) Minimum size limits for imported spiny lobster. There are two minimum size limits that apply to importation of spiny...

50 CFR 622.50 - Caribbean spiny lobster import prohibitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Caribbean spiny lobster import... ATLANTIC Management Measures § 622.50 Caribbean spiny lobster import prohibitions. (a) Minimum size limits for imported spiny lobster. There are two minimum size limits that apply to importation of spiny...

20 CFR 229.48 - Family maximum.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Family maximum. 229.48 Section 229.48... OVERALL MINIMUM GUARANTEE Computation of the Overall Minimum Rate § 229.48 Family maximum. (a) Family... month on one person's earnings record is limited. This limited amount is called the family maximum. The...

Determination of antibacterial flomoxef in serum by capillary electrophoresis.

PubMed

Kitahashi, Toshihiro; Furuta, Itaru

2003-04-01

A determination method of flomoxef (FMOX) concentration in serum by capillary electrophoresis is developed. Serum samples are extracted with acetonitrile. After pretreatment, they are separated in a fused-silica capillary tube with a 25 mM borate buffer (pH 10.0) as a running buffer that contains 50mM sodium dodecyl sulfate. The FMOX and acetaminophen (internal standard) are detected by UV absorbance at 200 nm. Linearity (0-200 mg/L) is good, and the minimum limit of detection is 1.0 mg/L (S/N = 3). The relative standard deviations of intra- and interassay variability are 1.60-4.78% and 2.10-3.31%, respectively, and the recovery rate is 84-98%. This method can be used for determination of FMOX concentration in serum.

Sagnac-interferometer-based fresnel flow probe.

PubMed

Tselikov, A; Blake, J

1998-10-01

We used a near-diffraction-limited flow or light-wave-interaction pipe to produce a Sagnac-interferometer-based Fresnel drag fluid flowmeter capable of detecting extremely small flow rates. An optimized design of the pipe along with the use of a state-of-the-art Sagnac interferometer results in a minimum-detectable water flow rate of 2.4 nl/s [1 drop/(5 h)]. The flowmeter's capability of measuring the water consumption by a small plant in real time has been demonstrated. We then designed an automated alignment system that finds and maintains the optimum fiber-coupling regime, which makes the applications of the Fresnel-drag-based flowmeters practical, especially if the length of the interaction pipe is long. Finally, we have applied the automatic alignment technique to an air flowmeter.

Ultimate detectability of volatile organic compounds: how much further can we reduce their ambient air sample volumes for analysis?

PubMed

Kim, Yong-Hyun; Kim, Ki-Hyun

2012-10-02

To understand the ultimately lowest detection range of volatile organic compounds (VOCs) in air, application of a high sensitivity analytical system was investigated by coupling thermal desorption (TD) technique with gas chromatography (GC) and time-of-flight (TOF) mass spectrometry (MS). The performance of the TD-GC/TOF MS system was evaluated using liquid standards of 19 target VOCs prepared in the range of 35 pg to 2.79 ng per μL. Studies were carried out using both total ion chromatogram (TIC) and extracted ion chromatogram (EIC) mode. EIC mode was used for calibration to reduce background and to improve signal-to-noise. The detectability of 19 target VOCs, if assessed in terms of method detection limit (MDL, per US EPA definition) and limit of detection (LOD), averaged 5.90 pg and 0.122 pg, respectively, with the mean coefficient of correlation (R(2)) of 0.9975. The minimum quantifiable mass of target analytes, when determined using real air samples by the TD-GC/TOF MS, is highly comparable to the detection limits determined experimentally by standard. In fact, volumes for the actual detection of the major aromatic VOCs like benzene, toluene, and xylene (BTX) in ambient air samples were as low as 1.0 mL in the 0.11-2.25 ppb range. It was thus possible to demonstrate that most target compounds including those in low abundance could be reliably quantified at concentrations down to 0.1 ppb at sample volumes of less than 10 mL. The unique sensitivity of this advanced analytical system can ultimately lead to a shift in field sampling strategy with smaller air sample volumes facilitating faster, simpler air sampling (e.g., use of gas syringes rather than the relative complexity of pumps or bags/canisters), with greatly reduced risk of analyte breakthrough and minimal interference, e.g., from atmospheric humidity. The improved detection limits offered by this system can also enhance accuracy and measurement precision.

Automated intelligent video surveillance system for ships

NASA Astrophysics Data System (ADS)

Wei, Hai; Nguyen, Hieu; Ramu, Prakash; Raju, Chaitanya; Liu, Xiaoqing; Yadegar, Jacob

2009-05-01

To protect naval and commercial ships from attack by terrorists and pirates, it is important to have automatic surveillance systems able to detect, identify, track and alert the crew on small watercrafts that might pursue malicious intentions, while ruling out non-threat entities. Radar systems have limitations on the minimum detectable range and lack high-level classification power. In this paper, we present an innovative Automated Intelligent Video Surveillance System for Ships (AIVS3) as a vision-based solution for ship security. Capitalizing on advanced computer vision algorithms and practical machine learning methodologies, the developed AIVS3 is not only capable of efficiently and robustly detecting, classifying, and tracking various maritime targets, but also able to fuse heterogeneous target information to interpret scene activities, associate targets with levels of threat, and issue the corresponding alerts/recommendations to the man-in- the-loop (MITL). AIVS3 has been tested in various maritime scenarios and shown accurate and effective threat detection performance. By reducing the reliance on human eyes to monitor cluttered scenes, AIVS3 will save the manpower while increasing the accuracy in detection and identification of asymmetric attacks for ship protection.

Ultimate Limit to the Spatial Resolution in Magnetic Imaging

NASA Astrophysics Data System (ADS)

Matthews, John; Wellstood, Frederick C.; Chatraphorn, Sojiphong

2003-03-01

Motivated by the continual improvement in the spatial resolution of source currents detected by magnetic field imaging, in particular scanning SQUID microscopy, we have determined a theoretical limit to the spatial resolution for a given set of parameters. The guiding principle here is that by adding known information (e.g. CAD diagram) about the source currents into the inversion algorithm, we reduce the number of unknown parameters and hence lower the uncertainty in the remaining parameters. We consider the ultimate limit to be the case where all the information about the system is known, except for a single parameter, e.g. the separation w of two long, straight wires each carrying a current I/2. For this particular example we find that for a current I=100;μA, with magnetic field noise Δ B=10 pT, at a standoff z=100;μm, the minimum resolvable separation is 2;μm, about an order of magnitude less than the present limit.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, D.A.; Keller, R.A.

1982-06-08

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be rlated to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10/sup -5/ cm/sup -1/ has been demonstrated using this technique.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, D.A.; Keller, R.A.

1985-10-01

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be related to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10[sup [minus]5] cm[sup [minus]1] has been demonstrated using this technique. 6 figs.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, David A.; Keller, Richard A.

1985-01-01

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be related to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10.sup.-5 cm.sup.-1 has been demonstrated using this technique.

Natural radionuclide and radiological assessment of building materials in high background radiation areas of Ramsar, Iran.

PubMed

Bavarnegin, Elham; Moghaddam, Masoud Vahabi; Fathabadi, Nasrin

2013-04-01

Building materials, collected from different sites in Ramsar, a northern coastal city in Iran, were analyzed for their natural radionuclide contents. The measurements were carried out using a high resolution high purity Germanium (HPGe) gamma-ray spectrometer system. The activity concentration of (226)Ra, (232)Th, and (40)K content varied from below the minimum detection limit up to 86,400 Bqkg(-1), 187 Bqkg(-1), and 1350 Bqkg(-1), respectively. The radiological hazards incurred from the use of these building materials were estimated through various radiation hazard indices. The result of this survey shows that values obtained for some samples are more than the internationally accepted maximum limits and as such, the use of them as a building material pose significant radiation hazard to individuals.

Searches for cosmic-ray electron anisotropies with the Fermi Large Area Telescope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ackermann, M.; Ajello, M.; Bechtol, K.

The Large Area Telescope on board the Fermi satellite (Fermi LAT) detected more than 1.6x10{sup 6} cosmic-ray electrons/positrons with energies above 60 GeV during its first year of operation. The arrival directions of these events were searched for anisotropies of angular scale extending from {approx}10 deg. up to 90 deg., and of minimum energy extending from 60 GeV up to 480 GeV. Two independent techniques were used to search for anisotropies, both resulting in null results. Upper limits on the degree of the anisotropy were set that depended on the analyzed energy range and on the anisotropy's angular scale. Themore » upper limits for a dipole anisotropy ranged from {approx}0.5% to {approx}10%.« less

Searches for cosmic-ray electron anisotropies with the Fermi Large Area Telescope

DOE PAGES

Ackermann, M.

2010-11-01

The Large Area Telescope on board the Fermi satellite (Fermi LAT) detected more than 1.6 × 10 6 cosmic-ray electrons/positrons with energies above 60 GeV during its first year of operation. The arrival directions of these events were searched for anisotropies of angular scale extending from ~ 10 ° up to 90°, and of minimum energy extending from 60 GeV up to 480 GeV. Two independent techniques were used to search for anisotropies, both resulting in null results. Upper limits on the degree of the anisotropy were set that depended on the analyzed energy range and on the anisotropy’s angularmore » scale. The upper limits for a dipole anisotropy ranged from ~ 0.5 % to ~ 10 % .« less

Cassini Radio Occultation by Enceladus Plume

NASA Astrophysics Data System (ADS)

Kliore, A.; Armstrong, J.; Flasar, F.; French, R.; Marouf, E.; Nagy, A.; Rappaport, N.; McGhee, C.; Schinder, P.; Anabtawi, A.; Asmar, S.; Barbinis, E.; Fleischman, D.; Goltz, G.; Aguilar, R.; Rochblatt, D.

2006-12-01

A fortuitous Cassini radio occultation by Enceladus plume occurs on September 15, 2006. The occultation track (the spacecraft trajectory in the plane of the sky as viewed from the Earth) has been designed to pass behind the plume (to pass above the south polar region of Enceladus) in a roughly symmetrical geometry centered on a minimum altitude above the surface of about 20 km. The minimum altitude was selected primarily to ensure probing much of the plume with good confidence given the uncertainty in the spacecraft trajectory. Three nearly-pure sinusoidal signals of 0.94, 3.6, and 13 cm-wavelength (Ka-, X-, and S-band, respectively) are simultaneously transmitted from Cassini and are monitored at two 34-m Earth receiving stations of the Deep Space Network (DSN) in Madrid, Spain (DSS-55 and DSS-65). The occultation of the visible plume is extremely fast, lasting less than about two minutes. The actual observation time extends over a much longer time interval, however, to provide a good reference baseline for potential detection of signal perturbations introduced by the tenuous neutral and ionized plume environment. Given the likely very small fraction of optical depth due to neutral particles of sizes larger than about 1 mm, detectable changes in signal intensity is perhaps unlikely. Detection of plume plasma along the radio path as perturbations in the signals frequency/phase is more likely and the magnitude will depend on the electron columnar density probed. The occultation time occurs not far from solar conjunction time (Sun-Earth-probe angle of about 33 degrees), causing phase scintillations due to the solar wind to be the primary limiting noise source. We estimate a delectability limit of about 1 to 3E16 electrons per square meter columnar density assuming about 100 seconds integration time. Potential measurement of the profile of electron columnar density along the occultation track is an exciting prospect at this time.

Determination of selected anions in water by ion chromatography

USGS Publications Warehouse

Fishman, Marvin J.; Pyen, Grace

1979-01-01

Ion chromatography is a rapid, sensitive, precise, and accurate method for the determination of major anions in rain water and surface waters. Simultaneous analyses of a single sample for bromide, chloride, fluoride, nitrate, nitrite, orthophosphate, and sulfate require approximately 20 minutes to obtain a chromatogram.Minimum detection limits range from 0.01 milligrams per liter for fluoride to 0.20 milligrams per liter for chloride and sulfate. Percent relative standard deviations were less than nine percent for all anions except nitrite in Standard Reference Water Samples. Only one reference sample contained nitrite and its concentration was near the minimum level of detection. Similar precision was found for chloride, nitrate, and sulfate at concentrations less than 5 milligrams per liter in rainfall samples. Precision for fluoride ranged from 12 to 22 percent, but is attributed to the low concentrations in these samples. The other anions were not detected.To determine accuracy of results, several samples were spiked with known concentrations of fluoride, chloride, nitrate, and sulfate; recoveries ranged from 96 to 103 percent. Known amounts of bromide and phosphate were added, separately, to several other waters, which contained bromide or phosphate. Recovery of added bromide and phosphate ranged from approximately 95 to 104 percent. No recovery data were obtained for nitrite.Chloride, nitrate, nitrite, orthophosphate, and sulfate, in several samples, were also determined independently by automated colorimetric procedures. An automated ion-selective electrode method was used to determine fluoride. Results are in agreement with results obtained by ion chromatography.

A comparison of six software packages for evaluation of solid lung nodules using semi-automated volumetry: what is the minimum increase in size to detect growth in repeated CT examinations.

PubMed

de Hoop, Bartjan; Gietema, Hester; van Ginneken, Bram; Zanen, Pieter; Groenewegen, Gerard; Prokop, Mathias

2009-04-01

We compared interexamination variability of CT lung nodule volumetry with six currently available semi-automated software packages to determine the minimum change needed to detect the growth of solid lung nodules. We had ethics committee approval. To simulate a follow-up examination with zero growth, we performed two low-dose unenhanced CT scans in 20 patients referred for pulmonary metastases. Between examinations, patients got off and on the table. Volumes of all pulmonary nodules were determined on both examinations using six nodule evaluation software packages. Variability (upper limit of the 95% confidence interval of the Bland-Altman plot) was calculated for nodules for which segmentation was visually rated as adequate. We evaluated 214 nodules (mean diameter 10.9 mm, range 3.3 mm-30.0 mm). Software packages provided adequate segmentation in 71% to 86% of nodules (p < 0.001). In case of adequate segmentation, variability in volumetry between scans ranged from 16.4% to 22.3% for the various software packages. Variability with five to six software packages was significantly less for nodules >or=8 mm in diameter (range 12.9%-17.1%) than for nodules <8 mm (range 18.5%-25.6%). Segmented volumes of each package were compared to each of the other packages. Systematic volume differences were detected in 11/15 comparisons. This hampers comparison of nodule volumes between software packages.

Improved Time-Lapsed Angular Scattering Microscopy of Single Cells

NASA Astrophysics Data System (ADS)

Cannaday, Ashley E.

By measuring angular scattering patterns from biological samples and fitting them with a Mie theory model, one can estimate the organelle size distribution within many cells. Quantitative organelle sizing of ensembles of cells using this method has been well established. Our goal is to develop the methodology to extend this approach to the single cell level, measuring the angular scattering at multiple time points and estimating the non-nuclear organelle size distribution parameters. The diameters of individual organelle-size beads were successfully extracted using scattering measurements with a minimum deflection angle of 20 degrees. However, the accuracy of size estimates can be limited by the angular range detected. In particular, simulations by our group suggest that, for cell organelle populations with a broader size distribution, the accuracy of size prediction improves substantially if the minimum angle of detection angle is 15 degrees or less. The system was therefore modified to collect scattering angles down to 10 degrees. To confirm experimentally that size predictions will become more stable when lower scattering angles are detected, initial validations were performed on individual polystyrene beads ranging in diameter from 1 to 5 microns. We found that the lower minimum angle enabled the width of this delta-function size distribution to be predicted more accurately. Scattering patterns were then acquired and analyzed from single mouse squamous cell carcinoma cells at multiple time points. The scattering patterns exhibit angular dependencies that look unlike those of any single sphere size, but are well-fit by a broad distribution of sizes, as expected. To determine the fluctuation level in the estimated size distribution due to measurement imperfections alone, formaldehyde-fixed cells were measured. Subsequent measurements on live (non-fixed) cells revealed an order of magnitude greater fluctuation in the estimated sizes compared to fixed cells. With our improved and better-understood approach to single cell angular scattering, we are now capable of reliably detecting changes in organelle size predictions due to biological causes above our measurement error of 20 nm, which enables us to apply our system to future studies of the investigation of various single cell biological processes.

How long is enough to detect terrestrial animals? Estimating the minimum trapping effort on camera traps

PubMed Central

Si, Xingfeng; Kays, Roland

2014-01-01

Camera traps is an important wildlife inventory tool for estimating species diversity at a site. Knowing what minimum trapping effort is needed to detect target species is also important to designing efficient studies, considering both the number of camera locations, and survey length. Here, we take advantage of a two-year camera trapping dataset from a small (24-ha) study plot in Gutianshan National Nature Reserve, eastern China to estimate the minimum trapping effort actually needed to sample the wildlife community. We also evaluated the relative value of adding new camera sites or running cameras for a longer period at one site. The full dataset includes 1727 independent photographs captured during 13,824 camera days, documenting 10 resident terrestrial species of birds and mammals. Our rarefaction analysis shows that a minimum of 931 camera days would be needed to detect the resident species sufficiently in the plot, and c. 8700 camera days to detect all 10 resident species. In terms of detecting a diversity of species, the optimal sampling period for one camera site was c. 40, or long enough to record about 20 independent photographs. Our analysis of evaluating the increasing number of additional camera sites shows that rotating cameras to new sites would be more efficient for measuring species richness than leaving cameras at fewer sites for a longer period. PMID:24868493

Physiological minimum temperatures for root growth in seven common European broad-leaved tree species.

PubMed

Schenker, Gabriela; Lenz, Armando; Körner, Christian; Hoch, Günter

2014-03-01

Temperature is the most important factor driving the cold edge distribution limit of temperate trees. Here, we identified the minimum temperatures for root growth in seven broad-leaved tree species, compared them with the species' natural elevational limits and identified morphological changes in roots produced near their physiological cold limit. Seedlings were exposed to a vertical soil-temperature gradient from 20 to 2 °C along the rooting zone for 18 weeks. In all species, the bulk of roots was produced at temperatures above 5 °C. However, the absolute minimum temperatures for root growth differed among species between 2.3 and 4.2 °C, with those species that reach their natural distribution limits at higher elevations also tending to have lower thermal limits for root tissue formation. In all investigated species, the roots produced at temperatures close to the thermal limit were pale, thick, unbranched and of reduced mechanical strength. Across species, the specific root length (m g(-1) root) was reduced by, on average, 60% at temperatures below 7 °C. A significant correlation of minimum temperatures for root growth with the natural high elevation limits of the investigated species indicates species-specific thermal requirements for basic physiological processes. Although these limits are not necessarily directly causative for the upper distribution limit of a species, they seem to belong to a syndrome of adaptive processes for life at low temperatures. The anatomical changes at the cold limit likely hint at the mechanisms impeding meristematic activity at low temperatures.

Detection of land mines by amplified fluorescence quenching of polymer films: a man-portable chemical sniffer for detection of ultratrace concentrations of explosives emanating from land mines

NASA Astrophysics Data System (ADS)

la Grone, Marcus J.; Cumming, Colin J.; Fisher, Mark E.; Fox, Michael J.; Jacob, Sheena; Reust, Dennis; Rockley, Mark G.; Towers, Eric

2000-08-01

The explosive charge within a landmine is the source for a mixture of chemical vapors that form a distinctive 'chemical signature' indicative of a landmine. The concentration of these compounds in the air over landmines is extremely low, well below the minimum detection limits of most field- portable chemical sensors. Described in this paper is a man- portable landmine detection system that has for the first time demonstrated the ability to detect landmines by direct sensing of the vapors of signature compounds in the air over landmines. The system utilizes fluorescent polymers developed by collaborators at the MIT. The sensor can detect ultra-trace concentrations of TNT vapor and other nitroaromatic compounds found in many landmine explosives. Thin films of the polymers exhibit intense fluorescence, but when exposed to vapors of nitroaromatic explosives the intensity of the light emitted from the films decreases. A single molecule of TNT binding to a receptor site quenches the fluorescence from many polymer repeat units, increasing the sensitivity by orders of magnitude. A sensor prototype has been develop that response in near real-time to low femtogram quantities of nitroaromatic explosives. The prototype is portable, lightweight, has low power consumption, is simple to operate, and is relatively inexpensive. Simultaneous field testing of the sensor and experienced canine landmine detection teams was recently completed. Although the testing was limited in scope, the performance of the senor met or exceeded that of the canines against buried landmines.

14 CFR 135.203 - VFR: Minimum altitudes.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false VFR: Minimum altitudes. 135.203 Section 135.203 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... Operating Limitations and Weather Requirements § 135.203 VFR: Minimum altitudes. Except when necessary for...

14 CFR 135.203 - VFR: Minimum altitudes.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false VFR: Minimum altitudes. 135.203 Section 135.203 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... Operating Limitations and Weather Requirements § 135.203 VFR: Minimum altitudes. Except when necessary for...

14 CFR 135.203 - VFR: Minimum altitudes.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false VFR: Minimum altitudes. 135.203 Section 135.203 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... Operating Limitations and Weather Requirements § 135.203 VFR: Minimum altitudes. Except when necessary for...

26 CFR 1.514(c)-2 - Permitted allocations under section 514(c)(9)(E).

Code of Federal Regulations, 2013 CFR

2013-04-01

.... (ii) Limitation on chargebacks of partial allocations. (3) Minimum gain chargebacks attributable to nonrecourse deductions. (4) Minimum gain chargebacks attributable to distribution of nonrecourse debt proceeds. (i) Chargebacks disregarded until allocations made. (ii) Certain minimum gain chargebacks related to...

26 CFR 1.514(c)-2 - Permitted allocations under section 514(c)(9)(E).

Code of Federal Regulations, 2011 CFR

2011-04-01

.... (ii) Limitation on chargebacks of partial allocations. (3) Minimum gain chargebacks attributable to nonrecourse deductions. (4) Minimum gain chargebacks attributable to distribution of nonrecourse debt proceeds. (i) Chargebacks disregarded until allocations made. (ii) Certain minimum gain chargebacks related to...

26 CFR 1.514(c)-2 - Permitted allocations under section 514(c)(9)(E).

Code of Federal Regulations, 2014 CFR

2014-04-01

.... (ii) Limitation on chargebacks of partial allocations. (3) Minimum gain chargebacks attributable to nonrecourse deductions. (4) Minimum gain chargebacks attributable to distribution of nonrecourse debt proceeds. (i) Chargebacks disregarded until allocations made. (ii) Certain minimum gain chargebacks related to...

26 CFR 1.514(c)-2 - Permitted allocations under section 514(c)(9)(E).

Code of Federal Regulations, 2012 CFR

2012-04-01

.... (ii) Limitation on chargebacks of partial allocations. (3) Minimum gain chargebacks attributable to nonrecourse deductions. (4) Minimum gain chargebacks attributable to distribution of nonrecourse debt proceeds. (i) Chargebacks disregarded until allocations made. (ii) Certain minimum gain chargebacks related to...

Constraining the High-Energy Emission from Gamma-Ray Bursts with Fermi

NASA Technical Reports Server (NTRS)

Gehrels, Neil; Harding, A. K.; Hays, E.; Racusin, J. L.; Sonbas, E.; Stamatikos, M.; Guirec, S.

2012-01-01

We examine 288 GRBs detected by the Fermi Gamma-ray Space Telescope's Gamma-ray Burst Monitor (GBM) that fell within the field-of-view of Fermi's Large Area Telescope (LAT) during the first 2.5 years of observations, which showed no evidence for emission above 100 MeV. We report the photon flux upper limits in the 0.1-10 GeV range during the prompt emission phase as well as for fixed 30 s and 100 s integrations starting from the trigger time for each burst. We compare these limits with the fluxes that would be expected from extrapolations of spectral fits presented in the first GBM spectral catalog and infer that roughly half of the GBM-detected bursts either require spectral breaks between the GBM and LAT energy bands or have intrinsically steeper spectra above the peak of the nuF(sub v) spectra (E(sub pk)). In order to distinguish between these two scenarios, we perform joint GBM and LAT spectral fits to the 30 brightest GBM-detected bursts and find that a majority of these bursts are indeed softer above E(sub pk) than would be inferred from fitting the GBM data alone. Approximately 20% of this spectroscopic subsample show statistically significant evidence for a cut-off in their high-energy spectra, which if assumed to be due to gamma gamma attenuation, places limits on the maximum Lorentz factor associated with the relativistic outflow producing this emission. All of these latter bursts have maximum Lorentz factor estimates that are well below the minimum Lorentz factors calculated for LAT-detected GRBs, revealing a wide distribution in the bulk Lorentz factor of GRB outflows and indicating that LAT-detected bursts may represent the high end of this distribution.

Constraining the high-energy emission from gamma-ray bursts with Fermi

DOE PAGES

Ackermann, M.; Ajello, M.; Baldini, L.; ...

2012-07-17

Here, we examine 288 gamma-ray bursts (GRBs) detected by the Fermi Gamma-ray Space Telescope's Gamma-ray Burst Monitor (GBM) that fell within the field of view of Fermi's Large Area Telescope (LAT) during the first 2.5 years of observations, which showed no evidence for emission above 100 MeV. We report the photon flux upper limits in the 0.1-10 GeV range during the prompt emission phase as well as for fixed 30 s and 100 s integrations starting from the trigger time for each burst. We also compare these limits with the fluxes that would be expected from extrapolations of spectral fitsmore » presented in the first GBM spectral catalog and infer that roughly half of the GBM-detected bursts either require spectral breaks between the GBM and LAT energy bands or have intrinsically steeper spectra above the peak of the νF ν spectra (E pk). In order to distinguish between these two scenarios, we perform joint GBM and LAT spectral fits to the 30 brightest GBM-detected bursts and find that a majority of these bursts are indeed softer above E pk than would be inferred from fitting the GBM data alone. Approximately 20% of this spectroscopic subsample show statistically significant evidence for a cutoff in their high-energy spectra, which if assumed to be due to γγ attenuation, places limits on the maximum Lorentz factor associated with the relativistic outflow producing this emission. Furthermore, all of these latter bursts have maximum Lorentz factor estimates that are well below the minimum Lorentz factors calculated for LAT-detected GRBs, revealing a wide distribution in the bulk Lorentz factor of GRB outflows and indicating that LAT-detected bursts may represent the high end of this distribution.« less

Constraining the High-energy Emission from Gamma-Ray Bursts with Fermi

NASA Astrophysics Data System (ADS)

Fermi Large Area Telescope Team; Ackermann, M.; Ajello, M.; Baldini, L.; Barbiellini, G.; Baring, M. G.; Bechtol, K.; Bellazzini, R.; Blandford, R. D.; Bloom, E. D.; Bonamente, E.; Borgland, A. W.; Bottacini, E.; Bouvier, A.; Brigida, M.; Buehler, R.; Buson, S.; Caliandro, G. A.; Cameron, R. A.; Cecchi, C.; Charles, E.; Chekhtman, A.; Chiang, J.; Ciprini, S.; Claus, R.; Cohen-Tanugi, J.; Cutini, S.; D'Ammando, F.; de Palma, F.; Dermer, C. D.; Silva, E. do Couto e.; Drell, P. S.; Drlica-Wagner, A.; Favuzzi, C.; Fukazawa, Y.; Fusco, P.; Gargano, F.; Gasparrini, D.; Gehrels, N.; Germani, S.; Giglietto, N.; Giordano, F.; Giroletti, M.; Glanzman, T.; Granot, J.; Grenier, I. A.; Grove, J. E.; Hadasch, D.; Hanabata, Y.; Harding, A. K.; Hays, E.; Horan, D.; Jóhannesson, G.; Kataoka, J.; Knödlseder, J.; Kocevski, D.; Kuss, M.; Lande, J.; Longo, F.; Loparco, F.; Lovellette, M. N.; Lubrano, P.; Mazziotta, M. N.; McEnery, J.; McGlynn, S.; Michelson, P. F.; Mitthumsiri, W.; Monzani, M. E.; Moretti, E.; Morselli, A.; Moskalenko, I. V.; Murgia, S.; Naumann-Godo, M.; Norris, J. P.; Nuss, E.; Nymark, T.; Ohsugi, T.; Okumura, A.; Omodei, N.; Orlando, E.; Panetta, J. H.; Parent, D.; Pelassa, V.; Pesce-Rollins, M.; Piron, F.; Pivato, G.; Racusin, J. L.; Rainò, S.; Rando, R.; Razzaque, S.; Reimer, A.; Reimer, O.; Ritz, S.; Ryde, F.; Sgrò, C.; Siskind, E. J.; Sonbas, E.; Spandre, G.; Spinelli, P.; Stamatikos, M.; Stawarz, Łukasz; Suson, D. J.; Takahashi, H.; Tanaka, T.; Thayer, J. G.; Thayer, J. B.; Tibaldo, L.; Tinivella, M.; Tosti, G.; Uehara, T.; Vandenbroucke, J.; Vasileiou, V.; Vianello, G.; Vitale, V.; Waite, A. P.; Fermi Gamma-ray Burst Monitor Team; Connaughton, V.; Briggs, M. S.; Guirec, S.; Goldstein, A.; Burgess, J. M.; Bhat, P. N.; Bissaldi, E.; Camero-Arranz, A.; Fishman, J.; Fitzpatrick, G.; Foley, S.; Gruber, D.; Jenke, P.; Kippen, R. M.; Kouveliotou, C.; McBreen, S.; Meegan, C.; Paciesas, W. S.; Preece, R.; Rau, A.; Tierney, D.; van der Horst, A. J.; von Kienlin, A.; Wilson-Hodge, C.; Xiong, S.

2012-08-01

We examine 288 gamma-ray bursts (GRBs) detected by the Fermi Gamma-ray Space Telescope's Gamma-ray Burst Monitor (GBM) that fell within the field of view of Fermi's Large Area Telescope (LAT) during the first 2.5 years of observations, which showed no evidence for emission above 100 MeV. We report the photon flux upper limits in the 0.1-10 GeV range during the prompt emission phase as well as for fixed 30 s and 100 s integrations starting from the trigger time for each burst. We compare these limits with the fluxes that would be expected from extrapolations of spectral fits presented in the first GBM spectral catalog and infer that roughly half of the GBM-detected bursts either require spectral breaks between the GBM and LAT energy bands or have intrinsically steeper spectra above the peak of the νF ν spectra (E pk). In order to distinguish between these two scenarios, we perform joint GBM and LAT spectral fits to the 30 brightest GBM-detected bursts and find that a majority of these bursts are indeed softer above E pk than would be inferred from fitting the GBM data alone. Approximately 20% of this spectroscopic subsample show statistically significant evidence for a cutoff in their high-energy spectra, which if assumed to be due to γγ attenuation, places limits on the maximum Lorentz factor associated with the relativistic outflow producing this emission. All of these latter bursts have maximum Lorentz factor estimates that are well below the minimum Lorentz factors calculated for LAT-detected GRBs, revealing a wide distribution in the bulk Lorentz factor of GRB outflows and indicating that LAT-detected bursts may represent the high end of this distribution.

All-optical photoacoustic microscopy (AOPAM) system for remote characterization of biological tissues

NASA Astrophysics Data System (ADS)

Sampathkumar, Ashwin; Chitnis, Parag V.; Silverman, Ronald H.

2014-03-01

Conventional photoacoustic microscopy (PAM) employs light pulses to produce a photoacoustic (PA) effect and detects the resulting acoustic waves using an ultrasound transducer acoustically coupled to the target. The resolution of conventional PAM is limited by the sensitivity and bandwidth of the ultrasound transducer. We investigated a versatile, all-optical PAM (AOPAM) system for characterizing in vivo as well as ex vivo biological specimens. The system employs non-contact interferometric detection of PA signals that overcomes limitations of conventional PAM. A 532-nm pump laser with a pulse duration of 5 ns excites the PA effect in tissue. Resulting acoustic waves produce surface displacements that are sensed using a 532-nm continuous-wave (CW) probe laser in a Michelson interferometer with a 1- GHz bandwidth. The pump and probe beams are coaxially focused using a 50X objective giving a diffraction-limited spot size of 0.48 μm. The phase-encoded probe beam is demodulated using homodyne methods. The detected timedomain signal is time reversed using k-space wave-propagation methods to produce a spatial distribution of PA sources in the target tissue. A minimum surface-displacement sensitivity of 0.19 pm was measured. PA-induced surface displacements are very small; therefore, they impose stringent detection requirements and determine the feasibility of implementing an all-optical PAM in biomedical applications. 3D PA images of ex vivo porcine retina specimens were generated successfully. We believe the AOPAM system potentially is well suited for assessing retinal diseases and other near-surface biomedical applications such as sectionless histology and evaluation of skin burns and pressure or friction ulcers.

Extreme Brightness Temperatures and Refractive Substructure in 3C273 with RadioAstron

NASA Astrophysics Data System (ADS)

Johnson, Michael D.; Kovalev, Yuri Y.; Gwinn, Carl R.; Gurvits, Leonid I.; Narayan, Ramesh; Macquart, Jean-Pierre; Jauncey, David L.; Voitsik, Peter A.; Anderson, James M.; Sokolovsky, Kirill V.; Lisakov, Mikhail M.

2016-03-01

Earth-space interferometry with RadioAstron provides the highest direct angular resolution ever achieved in astronomy at any wavelength. RadioAstron detections of the classic quasar 3C 273 on interferometric baselines up to 171,000 km suggest brightness temperatures exceeding expected limits from the “inverse-Compton catastrophe” by two orders of magnitude. We show that at 18 cm, these estimates most likely arise from refractive substructure introduced by scattering in the interstellar medium. We use the scattering properties to estimate an intrinsic brightness temperature of 7× {10}12 {{K}}, which is consistent with expected theoretical limits, but which is ˜15 times lower than estimates that neglect substructure. At 6.2 cm, the substructure influences the measured values appreciably but gives an estimated brightness temperature that is comparable to models that do not account for the substructure. At 1.35 {{cm}}, the substructure does not affect the extremely high inferred brightness temperatures, in excess of {10}13 {{K}}. We also demonstrate that for a source having a Gaussian surface brightness profile, a single long-baseline estimate of refractive substructure determines an absolute minimum brightness temperature, if the scattering properties along a given line of sight are known, and that this minimum accurately approximates the apparent brightness temperature over a wide range of total flux densities.

Ultrasensitive measurement of microcantilever displacement below the shot-noise limit

DOE PAGES

Pooser, Raphael C.; Lawrie, Benjamin J.

2015-04-23

The displacement of micro-electro-mechanical-systems (MEMs) cantilevers is used to measure a variety of phe- nomena in devices ranging from force microscopes for single spin detection[1] to biochemical sensors[2] to un- cooled thermal imaging systems[3]. The displacement readout is often performed optically with segmented de- tectors or interference measurements. Until recently, var- ious noise sources have limited the minimum detectable displacement in MEMs systems, but it is now possible to minimize all other sources[4] so that the noise level of the coherent light eld, called the shot noise limit (SNL), becomes the dominant source. Light sources dis- playing quantum-enhanced statistics belowmore » this limit are available[5, 6], with applications in gravitational wave astronomy[7] and bioimaging[8], but direct displacement measurements of MEMS cantilevers below the SNL have been impossible until now. Here, we demonstrate the rst direct measurement of a MEMs cantilever displace- ment with sub-SNL sensitivity, thus enabling ultratrace sensing, imaging, and microscopy applications. By com- bining multi-spatial-mode quantum light sources with a simple dierential measurement, we show that sub-SNL MEMs displacement sensitivity is highly accessible com- pared to previous eorts that measured the displacement of macroscopic mirrors with very distinct spatial struc- tures crafted with multiple optical parametric ampliers and locking loops[9]. We apply this technique to a com- mercially available microcantilever in order to detect dis- placements 60% below the SNL at frequencies where the microcantilever is shot-noise-limited. These results sup- port a new class of quantum MEMS sensor whose ulti- mate signal to noise ratio is determined by the correla- tions possible in quantum optics systems.« less

Ultrasensitive measurement of microcantilever displacement below the shot-noise limit

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pooser, Raphael C.; Lawrie, Benjamin J.

The displacement of micro-electro-mechanical-systems (MEMs) cantilevers is used to measure a variety of phe- nomena in devices ranging from force microscopes for single spin detection[1] to biochemical sensors[2] to un- cooled thermal imaging systems[3]. The displacement readout is often performed optically with segmented de- tectors or interference measurements. Until recently, var- ious noise sources have limited the minimum detectable displacement in MEMs systems, but it is now possible to minimize all other sources[4] so that the noise level of the coherent light eld, called the shot noise limit (SNL), becomes the dominant source. Light sources dis- playing quantum-enhanced statistics belowmore » this limit are available[5, 6], with applications in gravitational wave astronomy[7] and bioimaging[8], but direct displacement measurements of MEMS cantilevers below the SNL have been impossible until now. Here, we demonstrate the rst direct measurement of a MEMs cantilever displace- ment with sub-SNL sensitivity, thus enabling ultratrace sensing, imaging, and microscopy applications. By com- bining multi-spatial-mode quantum light sources with a simple dierential measurement, we show that sub-SNL MEMs displacement sensitivity is highly accessible com- pared to previous eorts that measured the displacement of macroscopic mirrors with very distinct spatial struc- tures crafted with multiple optical parametric ampliers and locking loops[9]. We apply this technique to a com- mercially available microcantilever in order to detect dis- placements 60% below the SNL at frequencies where the microcantilever is shot-noise-limited. These results sup- port a new class of quantum MEMS sensor whose ulti- mate signal to noise ratio is determined by the correla- tions possible in quantum optics systems.« less

Culture-free, highly sensitive, quantitative detection of bacteria from minimally processed samples using fluorescence imaging by smartphone.

PubMed

Shrivastava, Sajal; Lee, Won-Il; Lee, Nae-Eung

2018-06-30

A critical unmet need in the diagnosis of bacterial infections, which remain a major cause of human morbidity and mortality, is the detection of scarce bacterial pathogens in a variety of samples in a rapid and quantitative manner. Herein, we demonstrate smartphone-based detection of Staphylococcus aureus in a culture-free, rapid, quantitative manner from minimally processed liquid samples using aptamer-functionalized fluorescent magnetic nanoparticles. The tagged S. aureus cells were magnetically captured in a detection cassette, and then fluorescence was imaged using a smartphone camera with a light-emitting diode as the excitation source. Our results showed quantitative detection capability with a minimum detectable concentration as low as 10 cfu/ml by counting individual bacteria cells, efficiently capturing S. aureus cells directly from a peanut milk sample within 10 min. When the selectivity of detection was investigated using samples spiked with other pathogenic bacteria, no significant non-specific detection occurred. Furthermore, strains of S. aureus from various origins showed comparable results, ensuring that the approach can be widely adopted. Therefore, the quantitative fluorescence imaging platform on a smartphone could allow on-site detection of bacteria, providing great potential assistance during major infectious disease outbreaks in remote and resource-limited settings. Copyright © 2018 Elsevier B.V. All rights reserved.

Design for minimum energy in interstellar communication

NASA Astrophysics Data System (ADS)

Messerschmitt, David G.

2015-02-01

Microwave digital communication at interstellar distances is the foundation of extraterrestrial civilization (SETI and METI) communication of information-bearing signals. Large distances demand large transmitted power and/or large antennas, while the propagation is transparent over a wide bandwidth. Recognizing a fundamental tradeoff, reduced energy delivered to the receiver at the expense of wide bandwidth (the opposite of terrestrial objectives) is advantageous. Wide bandwidth also results in simpler design and implementation, allowing circumvention of dispersion and scattering arising in the interstellar medium and motion effects and obviating any related processing. The minimum energy delivered to the receiver per bit of information is determined by cosmic microwave background alone. By mapping a single bit onto a carrier burst, the Morse code invented for the telegraph in 1836 comes closer to this minimum energy than approaches used in modern terrestrial radio. Rather than the terrestrial approach of adding phases and amplitudes increases information capacity while minimizing bandwidth, adding multiple time-frequency locations for carrier bursts increases capacity while minimizing energy per information bit. The resulting location code is simple and yet can approach the minimum energy as bandwidth is expanded. It is consistent with easy discovery, since carrier bursts are energetic and straightforward modifications to post-detection pattern recognition can identify burst patterns. Time and frequency coherence constraints leading to simple signal discovery are addressed, and observations of the interstellar medium by transmitter and receiver constrain the burst parameters and limit the search scope.

Detecting Hardware-assisted Hypervisor Rootkits within Nested Virtualized Environments

DTIC Science & Technology

2012-06-14

least the minimum required for the guest OS and click “Next”. For 64-bit Windows 7 the minimum required is 2048 MB (Figure 66). Figure 66. Memory...prompted for Memory, allocate at least the minimum required for the guest OS, for 64-bit Windows 7 the minimum required is 2048 MB (Figure 79...130 21. Within the virtual disk creation wizard, select VDI for the file type (Figure 81). Figure 81. Select File Type 22. Select Dynamically

High Tensile Strength Amalgams for In-Space Fabrication and Repair

NASA Technical Reports Server (NTRS)

Grugel, Richard N.

2006-01-01

Amalgams are well known for their use in dental practice as a tooth filling material. They have a number of useful attributes that include room temperature fabrication, corrosion resistance, dimensional stability, and very good compressive strength. These properties well serve dental needs but, unfortunately, amalgams have extremely poor tensile strength, a feature that severely limits other potential applications. Improved material properties (strength and temperature) of amalgams may have application to the freeform fabrication of repairs or parts that might be necessary during an extended space mission. Advantages would include, but are not limited to: the ability to produce complex parts, a minimum number of processing steps, minimum crew interaction, high yield - minimum wasted material, reduced gravity compatibility, minimum final finishing, safety, and minimum power consumption. The work presented here shows how the properties of amalgams can be improved by changing particle geometries in conjunction with novel engineering metals.

Radon exhalation rate and natural radionuclide content in building materials of high background areas of Ramsar, Iran.

PubMed

Bavarnegin, E; Fathabadi, N; Vahabi Moghaddam, M; Vasheghani Farahani, M; Moradi, M; Babakhni, A

2013-03-01

Radon exhalation rates from building materials used in high background radiation areas (HBRA) of Ramsar were measured using an active radon gas analyzer with an emanation container. Radon exhalation rates from these samples varied from below the lower detection limit up to 384 Bq.m(-2) h(-1). The (226)Ra, (232)Th and (40)K contents were also measured using a high resolution HPGe gamma- ray spectrometer system. The activity concentration of (226)Ra, (232)Th and (40)K content varied from below the minimum detection limit up to 86,400 Bq kg(-1), 187 Bq kg(-1) and 1350 Bq kg(-1), respectively. The linear correlation coefficient between radon exhalation rate and radium concentration was 0.90. The result of this survey shows that radon exhalation rate and radium content in some local stones used as basements are extremely high and these samples are main sources of indoor radon emanation as well as external gamma radiation from uranium series. Copyright © 2012 Elsevier Ltd. All rights reserved.

Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples.

PubMed

Pikkemaat, M G; Rapallini, M L B A; Karp, M T; Elferink, J W A

2010-08-01

Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 microg kg(-1), while residue levels in most of the suspect samples were very low (<10 microg kg(-1)). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum.

Evaluation of Novel Broad-Range Real-Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens▿

PubMed Central

Vollmer, Tanja; Störmer, Melanie; Kleesiek, Knut; Dreier, Jens

2008-01-01

In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cryptococcus, Mucor, Penicillium, Pichia, Microsporum, Trichophyton, and Scopulariopsis. Species were identified in PCR-positive reactions by direct DNA sequencing. A noncompetitive internal control was applied to prevent false-negative results due to PCR inhibition. The minimum detection limit for the PCR was determined to be one 28S rDNA copy per PCR, and the 95% detection limit was calculated to 15 copies per PCR. To assess the clinical applicability of the PCR method, intensive-care patients with artificial respiration and patients with infective endocarditis were investigated. For this purpose, 76 tracheal secretion samples and 70 heart valve tissues were analyzed in parallel by real-time PCR and cultivation. No discrepancies in results were observed between PCR analysis and cultivation methods. Furthermore, the application of the PCR method was investigated for other clinical specimens, including cervical swabs, nail and horny skin scrapings, and serum, blood, and urine samples. The combination of a broad-range real-time PCR and direct sequencing facilitates rapid screening for fungal infection in various clinical specimens. PMID:18385440

Evaluation of novel broad-range real-time PCR assay for rapid detection of human pathogenic fungi in various clinical specimens.

PubMed

Vollmer, Tanja; Störmer, Melanie; Kleesiek, Knut; Dreier, Jens

2008-06-01

In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cryptococcus, Mucor, Penicillium, Pichia, Microsporum, Trichophyton, and Scopulariopsis. Species were identified in PCR-positive reactions by direct DNA sequencing. A noncompetitive internal control was applied to prevent false-negative results due to PCR inhibition. The minimum detection limit for the PCR was determined to be one 28S rDNA copy per PCR, and the 95% detection limit was calculated to 15 copies per PCR. To assess the clinical applicability of the PCR method, intensive-care patients with artificial respiration and patients with infective endocarditis were investigated. For this purpose, 76 tracheal secretion samples and 70 heart valve tissues were analyzed in parallel by real-time PCR and cultivation. No discrepancies in results were observed between PCR analysis and cultivation methods. Furthermore, the application of the PCR method was investigated for other clinical specimens, including cervical swabs, nail and horny skin scrapings, and serum, blood, and urine samples. The combination of a broad-range real-time PCR and direct sequencing facilitates rapid screening for fungal infection in various clinical specimens.

26 CFR 1.383-2 - Limitations on certain capital losses and excess credits in computing alternative minimum tax...

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Limitations on certain capital losses and excess credits in computing alternative minimum tax. [Reserved] 1.383-2 Section 1.383-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Insolvency...

40 CFR Table 3 to Subpart Jjjjjj... - Operating Limits for Boilers With Emission Limits

Code of Federal Regulations, 2013 CFR

2013-07-01

... as defined in § 63.11237. 4. Dry sorbent or activated carbon injection control Maintain the 30-day rolling average sorbent or activated carbon injection rate at or above the minimum sorbent injection rate or minimum activated carbon injection rate as defined in § 63.11237. When your boiler operates at...

Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction.

PubMed

Than, Leslie Thian Lung; Chong, Pei Pei; Ng, Kee Peng; Seow, Heng Fong

2015-01-01

The number of invasive candidiasis cases has risen especially with an increase in the number of immunosuppressed and immunocom promised patients. The early detection of Candida species which is specific and sensitive is important in determining the correct administration of antifungal drugs to patients. This study aims to develop a method for the detection, identification and quantitation of medically important Candida species through quantitative polymerase chain reaction (qPCR). The isocitrate lyase (ICL) gene which is not found in mammals was chosen as the target gene of real-time PCR. Absolute quantitation of the gene copy number was achieved by constructing the plasmid containing the ICL gene which is used to generate standard curve. Twenty fungal species, two bacterial species and human DNA were tested to check the specificity of the detection method. All eight Candida species were successfully detected, identified and quantitated based on the ICL gene. A seven-log range of the gene copy number and a minimum detection limit of 10(3) copies were achieved. A one-tube absolute quantification real-time PCR that differentiates medically important Candida species via individual unique melting temperature was achieved. Analytical sensitivity and specificity were not compromised.

Nonlinear optical detection of terahertz-wave radiation from resonant tunneling diodes.

PubMed

Takida, Yuma; Nawata, Kouji; Suzuki, Safumi; Asada, Masahiro; Minamide, Hiroaki

2017-03-06

The sensitive detection of terahertz (THz)-wave radiation from compact sources at room temperature is crucial for real-world THz-wave applications. Here, we demonstrate the nonlinear optical detection of THz-wave radiation from continuous-wave (CW) resonant tunneling diodes (RTDs) at 0.58, 0.78, and 1.14 THz. The up-conversion process in a MgO:LiNbO₃ crystal under the noncollinear phase-matching condition offers efficient wavelength conversion from a THz wave to a near-infrared (NIR) wave that is detected using a commercial NIR photodetector. The minimum detection limit of CW THz-wave power is as low as 5 nW at 1.14 THz, corresponding to 2-aJ energy and 2.7 × 10³ photons within the time window of a 0.31-ns pump pulse. Our results show that the input frequency and power of RTD devices can be calibrated by measuring the output wavelength and energy of up-converted waves, respectively. This optical detection technique for compact electronic THz-wave sources will open up a new opportunity for the realization of real-world THz-wave applications.

Gasdynamic Inlet Isolation in Rotating Detonation Engine

DTIC Science & Technology

2010-12-01

2D Total Variation Diminishing (TVD): Continuous Riemann Solver Minimum Dissipation: LHS & RHS Activate pressure switch : Supersonic Activate...Total Variation Diminishing (TVD) limiter: Continuous Riemann Solver Minimum Dissipation: LHS & RHS Activate pressure switch : Supersonic Activate...Continuous 94 Riemann Solver Minimum Dissipation: LHS & RHS Activate pressure switch : Supersonic Activate pressure gradient switch: Normal

14 CFR 135.221 - IFR: Alternate airport weather minimums.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false IFR: Alternate airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.221 IFR: Alternate airport weather minimums. No person may designate an alternate airport unless the weather reports or forecasts, or any...

14 CFR 135.221 - IFR: Alternate airport weather minimums.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false IFR: Alternate airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.221 IFR: Alternate airport weather minimums. No person may designate an alternate airport unless the weather reports or forecasts, or any...

14 CFR 135.221 - IFR: Alternate airport weather minimums.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false IFR: Alternate airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.221 IFR: Alternate airport weather minimums. No person may designate an alternate airport unless the weather reports or forecasts, or any...

14 CFR 135.221 - IFR: Alternate airport weather minimums.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false IFR: Alternate airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.221 IFR: Alternate airport weather minimums. No person may designate an alternate airport unless the weather reports or forecasts, or any...

14 CFR 135.221 - IFR: Alternate airport weather minimums.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false IFR: Alternate airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.221 IFR: Alternate airport weather minimums. No person may designate an alternate airport unless the weather reports or forecasts, or any...

Evaluation of a Dust Control for a Small Slab-Riding Dowel Drill for Concrete Pavement

PubMed Central

Echt, Alan; Mead, Kenneth

2016-01-01

Purpose To assess the effectiveness of local exhaust ventilation to control respirable crystalline silica exposures to acceptable levels during concrete dowel drilling. Approach Personal breathing zone samples for respirable dust and crystalline silica were collected while laborers drilled holes 3.5 cm diameter by 36 cm deep in a concrete slab using a single-drill slab-riding dowel drill equipped with local exhaust ventilation. Data were collected on air flow, weather, and productivity. Results All respirable dust samples were below the 90 µg detection limit which, when combined with the largest sample volume, resulted in a minimum detectable concentration of 0.31 mg m−3. This occurred in a 32-min sample collected when 27 holes were drilled. Quartz was only detected in one air sample; 0.09 mg m−3 of quartz was found on an 8-min sample collected during a drill maintenance task. The minimum detectable concentration for quartz in personal air samples collected while drilling was performed was 0.02 mg m−3. The average number of holes drilled during each drilling sample was 23. Over the course of the 2-day study, air flow measured at the dust collector decreased from 2.2 to 1.7 m3 s−1. Conclusions The dust control performed well under the conditions of this test. The initial duct velocity with a clean filter was sufficient to prevent settling, but gradually fell below the recommended value to prevent dust from settling in the duct. The practice of raising the drill between each hole may have prevented the dust from settling in the duct. A slightly higher flow rate and an improved duct design would prevent settling without regard to the position of the drill. PMID:26826033

Evaluation of a Dust Control for a Small Slab-Riding Dowel Drill for Concrete Pavement.

PubMed

Echt, Alan; Mead, Kenneth

2016-05-01

To assess the effectiveness of local exhaust ventilation to control respirable crystalline silica exposures to acceptable levels during concrete dowel drilling. Personal breathing zone samples for respirable dust and crystalline silica were collected while laborers drilled holes 3.5 cm diameter by 36 cm deep in a concrete slab using a single-drill slab-riding dowel drill equipped with local exhaust ventilation. Data were collected on air flow, weather, and productivity. All respirable dust samples were below the 90 µg detection limit which, when combined with the largest sample volume, resulted in a minimum detectable concentration of 0.31 mg m(-3). This occurred in a 32-min sample collected when 27 holes were drilled. Quartz was only detected in one air sample; 0.09 mg m(-3) of quartz was found on an 8-min sample collected during a drill maintenance task. The minimum detectable concentration for quartz in personal air samples collected while drilling was performed was 0.02 mg m(-3). The average number of holes drilled during each drilling sample was 23. Over the course of the 2-day study, air flow measured at the dust collector decreased from 2.2 to 1.7 m(3) s(-1). The dust control performed well under the conditions of this test. The initial duct velocity with a clean filter was sufficient to prevent settling, but gradually fell below the recommended value to prevent dust from settling in the duct. The practice of raising the drill between each hole may have prevented the dust from settling in the duct. A slightly higher flow rate and an improved duct design would prevent settling without regard to the position of the drill. Published by Oxford University Press on behalf of the British Occupational Hygiene Society 2016.

Magnetic resonance imaging for the detection, localisation, and characterisation of prostate cancer: recommendations from a European consensus meeting.

PubMed

Dickinson, Louise; Ahmed, Hashim U; Allen, Clare; Barentsz, Jelle O; Carey, Brendan; Futterer, Jurgen J; Heijmink, Stijn W; Hoskin, Peter J; Kirkham, Alex; Padhani, Anwar R; Persad, Raj; Puech, Philippe; Punwani, Shonit; Sohaib, Aslam S; Tombal, Bertrand; Villers, Arnauld; van der Meulen, Jan; Emberton, Mark

2011-04-01

Multiparametric magnetic resonance imaging (mpMRI) may have a role in detecting clinically significant prostate cancer in men with raised serum prostate-specific antigen levels. Variations in technique and the interpretation of images have contributed to inconsistency in its reported performance characteristics. Our aim was to make recommendations on a standardised method for the conduct, interpretation, and reporting of prostate mpMRI for prostate cancer detection and localisation. A consensus meeting of 16 European prostate cancer experts was held that followed the UCLA-RAND Appropriateness Method and facilitated by an independent chair. Before the meeting, 520 items were scored for "appropriateness" by panel members, discussed face to face, and rescored. Agreement was reached in 67% of 260 items related to imaging sequence parameters. T2-weighted, dynamic contrast-enhanced, and diffusion-weighted MRI were the key sequences incorporated into the minimum requirements. Consensus was also reached on 54% of 260 items related to image interpretation and reporting, including features of malignancy on individual sequences. A 5-point scale was agreed on for communicating the probability of malignancy, with a minimum of 16 prostatic regions of interest, to include a pictorial representation of suspicious foci. Limitations relate to consensus methodology. Dominant personalities are known to affect the opinions of the group and were countered by a neutral chairperson. Consensus was reached on a number of areas related to the conduct, interpretation, and reporting of mpMRI for the detection, localisation, and characterisation of prostate cancer. Before optimal dissemination of this technology, these outcomes will require formal validation in prospective trials. Copyright © 2010 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Measurement of nitrous acid (HONO) by external-cavity quantum cascade laser based quartz-enhanced photoacoustic absorption spectroscopy

NASA Astrophysics Data System (ADS)

Yi, Hongming; Maamary, Rabih; Gao, Xiaoming; Sigrist, Markus W.; Fertein, Eric; Chen, Weidong

2016-04-01

Spectroscopic detection of short-lived gaseous nitrous acid (HONO) at 1254.85 cm-1 was realized by off-beam coupled quartz-enhanced photoacoustic spectroscopy (QEPAS) in conjunction with an external cavity quantum cascade lasers (EC-QCL). High sensitivity monitoring of HONO was performed within a very small gas-sample volume (of ~40 mm3) allowing a significant reduction (of about 4 orders of magnitude) of air sampling residence time which is highly desired for accurate quantification of chemically reactive short-lived species. Calibration of the developed QEPAS-based HONO sensor was carried out by means of lab-generated HONO samples whose concentrations were determined by simultaneous measurements of direct HONO absorption spectra in a 109.5 m multipass cell using a distributed feedback (DBF) QCL. A minimum detection limit (MDL @ SNR=1) of 66 ppbv HONO was achieved at 70 mbar using a laser output power of 50 mW and 1 s integration time, which corresponded to a normalized noise equivalent absorption coefficient of 3.6×10-8 cm-1.W/Hz1/2. This MDL was down to 7 ppbv at the optimal integration time of 150 s. The corresponding minimum detected absorption coefficient (SNR=1) is ~1.1×10-7 cm-1 (MDL: ~3 ppbv) in 1 s and ~1.1×10-8 cm-1 (MDL~330 pptv) in 150 s, respectively, with 1 W laser power. Acknowledgements The authors acknowledge financial supports from the CaPPA project (ANR-10-LABX-005) and the CPER CLIMIBIO program. References H. Yi, R. Maamary, X. Gao, M. W. Sigrist, E. Fertein, W. Chen, "Short-lived species detection of nitrous acid by external-cavity quantum cascade laser based quartz-enhanced photoacoustic absorption spectroscopy", Appl. Phys. Lett. 106 (2015) 101109

Short-lived species detection of nitrous acid by external-cavity quantum cascade laser based quartz-enhanced photoacoustic absorption spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yi, Hongming; Laboratory of Atmospheric Physico-Chemistry, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, P.O. Box 1125, 350 Shushanhu Road, Hefei, Anhui 230031; Maamary, Rabih

2015-03-09

Spectroscopic detection of short-lived gaseous nitrous acid (HONO) at 1254.85 cm{sup −1} was realized by off-beam coupled quartz-enhanced photoacoustic spectroscopy (QEPAS) in conjunction with an external cavity quantum cascade lasers (EC-QCL). High sensitivity monitoring of HONO was performed within a very small gas-sample volume (of ∼40 mm{sup 3}) allowing a significant reduction (of about 4 orders of magnitude) of air sampling residence time which is highly desired for accurate quantification of chemically reactive short-lived species. Calibration of the developed QEPAS-based HONO sensor was carried out by means of lab-generated HONO samples whose concentrations were determined by direct absorption spectroscopy involving a ∼109.5 mmore » multipass cell and a distributed feedback QCL. A minimum detection limit (MDL) of 66 ppbv (1 σ) HONO was achieved at 70 mbar using a laser output power of 50 mW and 1 s integration time, which corresponded to a normalized noise equivalent absorption coefficient of 3.6 × 10{sup −8 }cm{sup −1} W/Hz{sup 1/2}. This MDL was down to 7 ppbv at the optimal integration time of 150 s. The corresponding 1σ minimum detected absorption coefficient is ∼1.1 × 10{sup −7 }cm{sup −1} (MDL ∼ 3 ppbv) in 1 s and ∼1.1 × 10{sup −8 }cm{sup −1} (MDL ∼ 330 pptv) in 150 s, respectively, with 1 W laser power.« less

Anti-D immunoglobulin preparations: the stability of anti-D concentrations and the error of the assay of anti-D.

PubMed

Hughes-Jones, N C; Hunt, V A; Maycock, W D; Wesley, E D; Vallet, L

1978-01-01

An analysis of the assay of 28 preparations of anti-D immunoglobulin using a radioisotope method carried out at 6-montly intervals for 2--4.5 years showed an average fall in anti-D concentration of 10.6% each year, with 99% confidence limits of 6.8--14.7%. The fall in anti-D concentration after storage at 37 degrees C for 1 month was less than 8%, the minimum change that could be detected. No significant change in physical characteristics of the immunoglobulin were detected. The error of a single estimate of anti-D by the radioisotope method (125I-labelled anti-IgG) used here was calculated to be such that the true value probably (p = 0.95) lay between 66 and 150% of the estimated value.

Sulfur speciation and sulfide oxidation in the water column of the Black Sea

NASA Astrophysics Data System (ADS)

Luther, George W., III; Church, Thomas M.; Powell, David

We have applied sulfur speciation techniques to understand the chemistry and cycling of sulfur in Black Sea waters. The only reduced dissolved inorganic sulfur species detected (above the low minimum detection limits of the voltammetric methods employed) in the water column was hydrogen sulfide. The maximum concentration of sulfide (423 μM) is similar to previous reports. Using a cathodic stripping square wave voltammetry (CSSWV) method for nanomolar levels of sulfide, we determined the precise boundary between the "free" hydrogen sulfide (sulfidic) zone and the upper (oxic/suboxic) water column at the two stations studied. This boundary has apparently moved up by about 50 m in the past 20 years. Our results help demonstrate three chemically distinct zones of water in the central basin of the Black Sea: (1) the oxic [0-65 m], (2) the anoxic/nonsulfidic [65-100 m] and (3) the sulfidic [>100 m]. Sulfide bound to metals ("complexed" sulfide) is observed in both the oxic and anoxic/nonsulfidic zones of the water column. This supports previous studies on metal sulfide forms. From the electrochemical data, it is possible to estimate the strength of the complexation of sulfide to metals (log K = 10 to 11). Thiosulfate and sulfite were below our minimum detectable limit (MDL) of 50 nM using CSSWV. Elemental sulfur (MDL 5 nM) was detected below the onset of the hydrogen sulfide zone (90-100 m) with a maximum of 30-60 nM near 120 m. The sulfur speciation results for the Black Sea are lower by one order of magnitude or more than other marine systems such as the Cariaco Trench and salt marshes. New HPLC techniques were applied to detect thiols at submicromolar levels. The presence of thiols (2-mercaptoethylamine, 2-mercaptoethanol, N-acetylcysteine and glutathione) is correlated with the remineralization of organic matter at the oxic and anoxic/nonsulfidic interface. Water samples collected from the upper 50 m of the sulfidic zone showed significant sulfide oxidation on storage onboard ship even though they were filtered (0.2 μm) and handled to exclude oxygen contamination. Chemical additives such as formaldehyde, glutaraldehyde, hydroxylamine and ascorbic acid prevented or retarded the sulfide loss. Thiosulfate and azide did not inhibit sulfide loss. These studies suggest an anaerobic chemical oxidation of sulfide rather than a biological oxidation on stored and filtered samples.

Physiological and biochemical responses of Prorocentrum minimum to high light stress

NASA Astrophysics Data System (ADS)

Park, So Yun; Choi, Eun Seok; Hwang, Jinik; Kim, Donggiun; Ryu, Tae Kwon; Lee, Taek-Kyun

2009-12-01

Prorocentrum minimum is a common bloomforming photosynthetic dinoflagellate found along the southern coast of Korea. To investigate the adaptive responses of P. minimum to high light stress, we measured growth rate, and generation of reactive oxidative species (ROS), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in cultures exposed to normal (NL) and high light levels (HL). The results showed that HL (800 μmol m-2 s-1) inhibited growth of P. minimum, with maximal inhibition after 7-9 days. HL also increased the amount of ROS and MDA, suggesting that HL stress leads to oxidative damage and lipid peroxidation in this species. Under HL, we first detected superoxide on day 4 and H2O2 on day 5. We also detected SOD activity on day 5 and CAT activity on day 6. The level of lipid peroxidation, an indicator of cell death, was high on day 8. Addition of diphenyleneiodonium (DPI), an NAD(P)H inhibitor, decreased the levels of superoxide generation and lipid peroxidation. Our results indicate that the production of ROS which results from HL stress in P. minimum also induces antioxidative enzymes that counteract oxidative damage and allow P. minimum to survive.

Optimizing occupancy surveys by maximizing detection probability: application to amphibian monitoring in the Mediterranean region.

PubMed

Petitot, Maud; Manceau, Nicolas; Geniez, Philippe; Besnard, Aurélien

2014-09-01

Setting up effective conservation strategies requires the precise determination of the targeted species' distribution area and, if possible, its local abundance. However, detection issues make these objectives complex for most vertebrates. The detection probability is usually <1 and is highly dependent on species phenology and other environmental variables. The aim of this study was to define an optimized survey protocol for the Mediterranean amphibian community, that is, to determine the most favorable periods and the most effective sampling techniques for detecting all species present on a site in a minimum number of field sessions and a minimum amount of prospecting effort. We visited 49 ponds located in the Languedoc region of southern France on four occasions between February and June 2011. Amphibians were detected using three methods: nighttime call count, nighttime visual encounter, and daytime netting. The detection nondetection data obtained was then modeled using site-occupancy models. The detection probability of amphibians sharply differed between species, the survey method used and the date of the survey. These three covariates also interacted. Thus, a minimum of three visits spread over the breeding season, using a combination of all three survey methods, is needed to reach a 95% detection level for all species in the Mediterranean region. Synthesis and applications: detection nondetection surveys combined to site occupancy modeling approach are powerful methods that can be used to estimate the detection probability and to determine the prospecting effort necessary to assert that a species is absent from a site.

Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR.

PubMed

Decraene, Charles; Silveira, Amanda B; Bidard, François-Clément; Vallée, Audrey; Michel, Marc; Melaabi, Samia; Vincent-Salomon, Anne; Saliou, Adrien; Houy, Alexandre; Milder, Maud; Lantz, Olivier; Ychou, Marc; Denis, Marc G; Pierga, Jean-Yves; Stern, Marc-Henri; Proudhon, Charlotte

2018-02-01

Progress in the liquid biopsy field, combined with the development of droplet digital PCR (ddPCR), has enabled noninvasive monitoring of mutations with high detection accuracy. However, current assays detect a restricted number of mutations per reaction. ddPCR is a recognized method for detecting alterations previously characterized in tumor tissues, but its use as a discovery tool when the mutation is unknown a priori remains limited. We established 2 ddPCR assays detecting all genomic alterations within KRAS exon 2 and EGFR exon 19 mutation hotspots, which are of clinical importance in colorectal and lung cancer, with use of a unique pair of TaqMan ® oligoprobes. The KRAS assay scanned for the 7 most common mutations in codons 12/13 but also all other mutations found in that region. The EGFR assay screened for all in-frame deletions of exon 19, which are frequent EGFR-activating events. The KRAS and EGFR assays were highly specific and both reached a limit of detection of <0.1% in mutant allele frequency. We further validated their performance on multiple plasma and formalin-fixed and paraffin-embedded tumor samples harboring a panel of different KRAS or EGFR mutations. This method presents the advantage of detecting a higher number of mutations with single-reaction ddPCRs while consuming a minimum of patient sample. This is particularly useful in the context of liquid biopsy because the amount of circulating tumor DNA is often low. This method should be useful as a discovery tool when the tumor tissue is unavailable or to monitor disease during therapy. © 2017 American Association for Clinical Chemistry.

Analysis of Aircraft Fuels and Related Materials

DTIC Science & Technology

1982-09-01

content by the Karl Fischer method . Each 2040 solvent sample represented a different step in a clean-up procedure conducted by Aero Propulsion...izes a potentiometric titration with alcoholic silver nitrate. This method has a minimum detectability of 1 ppm. It has a re- peatability of 0.1 ppm... Method 163-80, which util- izes a potentiometric titration with alcoholic silver nitrate. This method has a minimum detectability of 1 ppm and has a

Flexible gas sensor based on graphene/ethyl cellulose nanocomposite with ultra-low strain response for volatile organic compounds rapid detection

NASA Astrophysics Data System (ADS)

Zhang, Qiankun; An, Chunhua; Fan, Shuangqing; Shi, Sigang; Zhang, Rongjie; Zhang, Jing; Li, Quanning; Zhang, Daihua; Hu, Xiaodong; Liu, Jing

2018-07-01

Minimizing the strain-induced undesirable effects is one of the major efforts to be made for flexible electronics. This work demonstrates a highly sensitive flexible gas sensor with ultra-low strain response, which is potentially suitable for wearable electronics applications. The gas sensing material is a free-standing and flexible thin film made of graphene/ethyl cellulose (EC) nanocomposite, which is then integrated with flexible substrate of polyethylene terephthalate. The sensor exhibits relative resistance change within 0.3% at a minimum bending radius of 3.18 mm and 0.2% at the bending radius of 5 mm after 400 bending cycles. The limited strain response attributes to several applied strategies, including using EC with high Young’s modulus as the matrix material, maintaining high graphene concentration and adopting suspended device structure. In contrast to the almost negligible strain sensitivity, the sensor presents large and rapid responses toward volatile organic compounds (VOCs) at room temperature. Specifically, the sensor resistance rapidly increases upon the exposure to VOCs with detection limits ranging from 37 to 167 ppm. A preliminary demo of wearable gas sensing capability is also implemented by wearing the sensor on human hand, which successfully detects several VOCs, instead of normal hand gestures.

Flexible gas sensor based on graphene/ethyl cellulose nanocomposite with ultra-low strain response for volatile organic compounds rapid detection.

PubMed

Zhang, Qiankun; An, Chunhua; Fan, Shuangqing; Shi, Sigang; Zhang, Rongjie; Zhang, Jing; Li, Quanning; Zhang, Daihua; Hu, Xiaodong; Liu, Jing

2018-04-18

Minimizing the strain-induced undesirable effects is one of the major efforts to be made for flexible electronics. This work demonstrates a highly sensitive flexible gas sensor with ultra-low strain response, which is potentially suitable for wearable electronics applications. The gas sensing material is a free-standing and flexible thin film made of graphene/ethyl cellulose (EC) nanocomposite, which is then integrated with flexible substrate of polyethylene terephthalate. The sensor exhibits relative resistance change within 0.3% at a minimum bending radius of 3.18 mm and 0.2% at the bending radius of 5 mm after 400 bending cycles. The limited strain response attributes to several applied strategies, including using EC with high Young's modulus as the matrix material, maintaining high graphene concentration and adopting suspended device structure. In contrast to the almost negligible strain sensitivity, the sensor presents large and rapid responses toward volatile organic compounds (VOCs) at room temperature. Specifically, the sensor resistance rapidly increases upon the exposure to VOCs with detection limits ranging from 37 to 167 ppm. A preliminary demo of wearable gas sensing capability is also implemented by wearing the sensor on human hand, which successfully detects several VOCs, instead of normal hand gestures.

Sub-micron Hard X-ray Fluorescence Imaging of Synthetic Elements

PubMed Central

Jensen, Mark P.; Aryal, Baikuntha P.; Gorman-Lewis, Drew; Paunesku, Tatjana; Lai, Barry; Vogt, Stefan; Woloschak, Gayle E.

2013-01-01

Synchrotron-based X-ray fluorescence microscopy (SXFM) using hard X-rays focused into sub-micron spots is a powerful technique for elemental quantification and mapping, as well as microspectroscopic measurement such as μ-XANES (X-ray absorption near edge structure). We have used SXFM to image and simultaneously quantify the transuranic element plutonium at the L3 or L2 edge as well as lighter biologically essential elements in individual rat pheochromocytoma (PC12) cells after exposure to the long-lived plutonium isotope 242Pu. Elemental maps reveal that plutonium localizes principally in the cytoplasm of the cells and avoids the cell nucleus, which is marked by the highest concentrations of phosphorus and zinc, under the conditions of our experiments. The minimum detection limit under typical acquisition conditions for an average 202 μm2 cell is 1.4 fg Pu/cell or 2.9 × 10−20 moles Pu/μm2, which is similar to the detection limit of K-edge SXFM of transition metals at 10 keV. Copper electron microscopy grids were used to avoid interference from gold X-ray emissions, but traces of strontium present in naturally occurring calcium can still interfere with plutonium detection using its Lα X-ray emission. PMID:22444530

A new tactics for the detection of S. aureus via paper based geno-interface incorporated with graphene nano dots and zeolites.

PubMed

Mathur, Ashish; Gupta, Rathin; Kondal, Sidharth; Wadhwa, Shikha; Pudake, Ramesh N; Shivani; Kansal, Ruby; Pundir, C S; Narang, Jagriti

2018-06-01

Staphylococcus aureus (S. aureus) is a pathogenic bacteria which causes infectious diseases and food poisoning. Current diagnostic methods for infectious disease require sophisticated instruments, long analysis time and expensive reagents which restrict their application in resource-limited settings. Electrochemical paper based analytical device (EPAD) was developed by integrating graphene nano dots (GNDs) and zeolite (Zeo) using specific DNA probe. The ssDNA/GNDs-Zeo modified paper based analytical device (PAD) was characterized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The genosensor was optimized at pH7.4 and incubation temperature of 30°C. A linear current response with respect to target DNA concentrations was obtained. The limit of detection (LOD) of the proposed sensor was found out to be 0.1nM. The specificity was confirmed by introducing non-complimentary target DNA to ssDNA/GNDs-Zeo modified PAD. The suitability of the proposed EPAD genosensor was demonstrated with fruit juice samples mixed with S. aureus. The proposed EPAD genosensor is a low cost, highly specific, easy to fabricate diagnostic device for detection of S. aureus bacteria which requires very low sample volume and minimum analysis time of 10s. Copyright © 2018 Elsevier B.V. All rights reserved.

On the impacts of computing daily temperatures as the average of the daily minimum and maximum temperatures

NASA Astrophysics Data System (ADS)

Villarini, Gabriele; Khouakhi, Abdou; Cunningham, Evan

2017-12-01

Daily temperature values are generally computed as the average of the daily minimum and maximum observations, which can lead to biases in the estimation of daily averaged values. This study examines the impacts of these biases on the calculation of climatology and trends in temperature extremes at 409 sites in North America with at least 25 years of complete hourly records. Our results show that the calculation of daily temperature based on the average of minimum and maximum daily readings leads to an overestimation of the daily values of 10+ % when focusing on extremes and values above (below) high (low) thresholds. Moreover, the effects of the data processing method on trend estimation are generally small, even though the use of the daily minimum and maximum readings reduces the power of trend detection ( 5-10% fewer trends detected in comparison with the reference data).

Evaluation of Precipitation Detection over Various Surfaces from Passive Microwave Imagers and Sounders

NASA Technical Reports Server (NTRS)

Munchak, S. Joseph; Skofronick-Jackson, Gail

2012-01-01

During the middle part of this decade a wide variety of passive microwave imagers and sounders will be unified in the Global Precipitation Measurement (GPM) mission to provide a common basis for frequent (3 hr), global precipitation monitoring. The ability of these sensors to detect precipitation by discerning it from non-precipitating background depends upon the channels available and characteristics of the surface and atmosphere. This study quantifies the minimum detectable precipitation rate and fraction of precipitation detected for four representative instruments (TMI, GMI, AMSU-A, and AMSU-B) that will be part of the GPM constellation. Observations for these instruments were constructed from equivalent channels on the SSMIS instrument on DMSP satellites F16 and F17 and matched to precipitation data from NOAA's National Mosaic and QPE (NMQ) during 2009 over the continuous United States. A variational optimal estimation retrieval of non-precipitation surface and atmosphere parameters was used to determine the consistency between the observed brightness temperatures and these parameters, with high cost function values shown to be related to precipitation. The minimum detectable precipitation rate, defined as the lowest rate for which probability of detection exceeds 50%, and the detected fraction of precipitation, are reported for each sensor, surface type (ocean, coast, bare land, snow cover) and precipitation type (rain, mix, snow). The best sensors over ocean and bare land were GMI (0.22 mm/hr minimum threshold and 90% of precipitation detected) and AMSU (0.26 mm/hr minimum threshold and 81% of precipitation detected), respectively. Over coasts (0.74 mm/hr threshold and 12% detected) and snow-covered surfaces (0.44 mm/hr threshold and 23% detected), AMSU again performed best but with much lower detection skill, whereas TMI had no skill over these surfaces. The sounders (particularly over water) benefited from the use of re-analysis data (vs. climatology) to set the a-priori atmospheric state and all instruments benefit from the use of a conditional snow cover emissivity database over land. It is recommended that real-time sources of these data be used in the operational GPM precipitation algorithms.

Comparison of natural estrogen removal efficiency in the conventional activated sludge process and the oxidation ditch process.

PubMed

Hashimoto, T; Onda, K; Nakamura, Y; Tada, K; Miya, A; Murakami, T

2007-05-01

The presence of natural estrogens, 17beta-estradiol (E2), estrone (E1) and estriol (E3), as well as estrogenic activity in wastewater influents and secondary effluents were investigated in 20 full-scale wastewater treatment plants in Japan. In all of the influent samples, natural estrogens were detected at concentrations above the minimum limits of detection (0.5ng/L). The concentrations of natural estrogens detected in the effluent of oxidation ditch plants were generally lower than previously reported values. On the other hand, in the conventional activated sludge plants, increments of E1 during biological treatment were frequently observed although E2 and E3 were removed effectively in the process. The removal rates of natural estrogens or estrogenic activity show no observed statistical relationship with the solids retention time (SRT) and the hydraulic retention time (HRT). However, the plants with high SRT or HRT generally showed high and stable removal of both natural estrogens and estrogenic activity.

Determination of the occurrence of gold in an unoxidized Carlin-type ore sample using synchrotron radiation

USGS Publications Warehouse

Chen, J.R.; Chao, E.C.T.; Minkin, J.A.; Back, J.M.; Bagby, W.C.; Rivers, M.L.; Sutton, S.R.; Gordon, B.M.; Hanson, A.L.; Jones, K.W.

1987-01-01

The occurrence of the so-called invisible gold in two unoxidized Carlin-type gold samples from Nevada has been determined using synchrotron X-ray fluorescence (SXRF) analysis at the National Synchrotron Light Source, Brookhaven National Laboratory. A bedded sample from the East ore zone of the Carlin deposit and a breccia sample from Horse Canyon were analyzed. Preliminary results show that gold is found only in the Horse Canyon breccia sample. Experimental details including other X-ray line and diffraction peak interferences, standards used, and minimum detection limits (MDLs) are discussed. Gold, with a MDL range of 0.8 to 3 ppm, was not detected in euhedral pyrite crystals except in the interior porous portion of one grain. Gold was detected in some parts of the matrix. The phase which contains gold has not yet been identified. The highest content of gold so far analyzed is about 40 ppm. There are interesting implications of these new findings. ?? 1987.

Evaluation of back scatter interferometry, a method for detecting protein binding in solution.

PubMed

Jepsen, S T; Jørgensen, T M; Zong, W; Trydal, T; Kristensen, S R; Sørensen, H S

2015-02-07

Back Scatter Interferometry (BSI) has been proposed to be a highly sensitive and versatile refractive index sensor usable for analytical detection of biomarker and protein interactions in solution. However the existing literature on BSI lacks a physical explanation of why protein interactions in general should contribute to the BSI signal. We have established a BSI system to investigate this subject in further detail. We contribute with a thorough analysis of the robustness of the sensor including unwanted contributions to the interferometric signal caused by temperature variation and dissolved gasses. We report a limit of the effective minimum detectability of refractive index at the 10(-7) level. Long term stability was examined by simultaneously monitoring the temperature inside the capillary revealing an average drift of 2.0 × 10(-7) per hour. Finally we show that measurements on protein A incubated with immunoglobulin G do not result in a signal that can be attributed to binding affinities as otherwise claimed in literature.

Polarimetric Analysis of the Long Duration Gamma-Ray Burst GRB 160530A With the Balloon Borne Compton Spectrometer and Imager

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lowell, A. W.; Boggs, S. E; Chiu, C. L.

2017-10-20

A long duration gamma-ray burst, GRB 160530A, was detected by the Compton Spectrometer and Imager (COSI) during the 2016 COSI Super Pressure Balloon campaign. As a Compton telescope, COSI is inherently sensitive to the polarization of gamma-ray sources in the energy range 0.2–5.0 MeV. We measured the polarization of GRB 160530A using (1) a standard method (SM) based on fitting the distribution of azimuthal scattering angles with a modulation curve and (2) an unbinned, maximum likelihood method (MLM). In both cases, the measured polarization level was below the 99% confidence minimum detectable polarization levels of 72.3% ± 0.8% (SM) andmore » 57.5% ± 0.8% (MLM). Therefore, COSI did not detect polarized gamma-ray emission from this burst. Our most constraining 90% confidence upper limit on the polarization level was 46% (MLM).« less

Fiber-ring laser-based intracavity photoacoustic spectroscopy for trace gas sensing.

PubMed

Wang, Qiang; Wang, Zhen; Chang, Jun; Ren, Wei

2017-06-01

We demonstrated a novel trace gas sensing method based on fiber-ring laser intracavity photoacoustic spectroscopy. This spectroscopic technique is a merging of photoacoustic spectroscopy (PAS) with a fiber-ring cavity for sensitive and all-fiber gas detection. A transmission-type PAS gas cell (resonant frequency f₀=2.68  kHz) was placed inside the fiber-ring laser to fully utilize the intracavity laser power. The PAS signal was excited by modulating the laser wavelength at f₀/2 using a custom-made fiber Bragg grating-based modulator. We used this spectroscopic technique to detect acetylene (C₂H₂) at 1531.6 nm as a proof of principle. With a low Q-factor (4.9) of the PAS cell, our sensor achieved a good linear response (R²=0.996) to C₂H₂ concentration and a minimum detection limit of 390 ppbv at 2-s response time.

Multiplex real-time PCR assay for detection of pathogenic Vibrio parahaemolyticus strains.

PubMed

He, Peiyan; Chen, Zhongwen; Luo, Jianyong; Wang, Henghui; Yan, Yong; Chen, Lixia; Gao, Wenjie

2014-01-01

Foodborne disease caused by pathogenic Vibrio parahaemolyticus has become a serious public health problem in many countries. Rapid diagnosis and the identification of pathogenic V. parahaemolyticus are very important in the context of public health. In this study, an EvaGreen-based multiplex real-time PCR assay was established for the detection of pathogenic V. parahaemolyticus. This assay targeted three genetic markers of V. parahaemolyticus (species-specific gene toxR and virulence genes tdh and trh). The assay could unambiguously identify pathogenic V. parahaemolyticus with a minimum detection limit of 1.4 pg genomic DNA per reaction (concentration giving a positive multiplex real-time PCR result in 95% of samples). The specificity of the assay was evaluated using 72 strains of V. parahaemolyticus and other bacteria. A validation of the assay with clinical samples confirmed its sensitivity and specificity. Our data suggest the newly established multiplex real-time PCR assay is practical, cost-effective, specific, sensitive and capable of high-throughput detection of pathogenic V. parahaemolyticus. Copyright © 2014. Published by Elsevier Ltd.

Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA.

PubMed

Miyake, Mayo; Nakajima, Hizuru; Hemmi, Akihide; Yahiro, Masayuki; Adachi, Chihaya; Soh, Nobuaki; Ishimatsu, Ryoichi; Nakano, Koji; Uchiyama, Katsumi; Imato, Toshihiko

2012-07-15

The performance of an organic thin film photodiode (OPD), fabricated from a hetero-junction comprised of two layers of C(60) and a phthalocyanine-Cu(II) complex was evaluated by detecting the chemiluminescence generated from the reaction of luminol with horseradish peroxidase in the presence of H(2)O(2), and the fluorescence from resorufin, as an optical detector. The photocurrent of the OPD was linear with respect to the power of light from a commercial LED. The sensitivity of the OPD was sufficient for detecting chemiluminescence with a power 0.1μW/cm(2). The OPD was successfully used in a flow-immunoassay for IgA, a marker of human stress, in which a sandwich immunoassay was carried out on the microchip and the fluorescence from resorufin, produced by the enzymatic reaction, was detected. The detection limits for resorufin and IgA were 5.0μM and 16ng/mL, respectively. The photosensitivity of the OPD remained relatively constant for a minimum of one year. Copyright © 2012 Elsevier B.V. All rights reserved.

[Trace detection of ammonia at 1.531 microm].

PubMed

Jia, Hui; Guo, Xiao-Yong; Cai, Ting-Dong; Zhao, Wei-Xiong; Wang, Lei; Tan, Tu; Zhang, Wei-Jun; Gao, Xiao-Ming

2009-12-01

A compact instrument based on the off-axis integrated-cavity output spectroscopy (ICOS) technology was developed for sensitive measurements of gas mixing ratios (ammonia in air) at room temperature by using fiber-coupled distributed feedback (DFB) diode laser operating at 1.531 microm. The absorption line of ammonia at 6 528.764 cm(-1) was chosen for trace detection. The mirrors' effective reflectivity R2 of 0.996 9 was first calibrated by carbon dioxide under this condition, and the cavity 35.8 cm in length as an absorption cell could yield an optical path of presumably 115.46 m. As a result, a minimum detectable concentration of approximately 2.66 ppmv (S/N-3) at the total pressure of 100 torr was obtained. Then the lock-in amplifier was added in the system to acquire the second harmonic signal by combination of wavelength modulation technology, which could better suppress background noise and improve the signal-to-noise ratio, and a detection limit of 0.293 ppmv (S/N-3) was achieved eventually. This work demonstrated the potential of the system for a range of atmospheric species sensing in the future.

The Effect of Minimum Wage Rates on High School Completion

ERIC Educational Resources Information Center

Warren, John Robert; Hamrock, Caitlin

2010-01-01

Does increasing the minimum wage reduce the high school completion rate? Previous research has suffered from (1. narrow time horizons, (2. potentially inadequate measures of states' high school completion rates, and (3. potentially inadequate measures of minimum wage rates. Overcoming each of these limitations, we analyze the impact of changes in…

The absolute threshold of cone vision

PubMed Central

Koeing, Darran; Hofer, Heidi

2013-01-01

We report measurements of the absolute threshold of cone vision, which has been previously underestimated due to sub-optimal conditions or overly strict subjective response criteria. We avoided these limitations by using optimized stimuli and experimental conditions while having subjects respond within a rating scale framework. Small (1′ fwhm), brief (34 msec), monochromatic (550 nm) stimuli were foveally presented at multiple intensities in dark-adapted retina for 5 subjects. For comparison, 4 subjects underwent similar testing with rod-optimized stimuli. Cone absolute threshold, that is, the minimum light energy for which subjects were just able to detect a visual stimulus with any response criterion, was 203 ± 38 photons at the cornea, ∼0.47 log units lower than previously reported. Two-alternative forced-choice measurements in a subset of subjects yielded consistent results. Cone thresholds were less responsive to criterion changes than rod thresholds, suggesting a limit to the stimulus information recoverable from the cone mosaic in addition to the limit imposed by Poisson noise. Results were consistent with expectations for detection in the face of stimulus uncertainty. We discuss implications of these findings for modeling the first stages of human cone vision and interpreting psychophysical data acquired with adaptive optics at the spatial scale of the receptor mosaic. PMID:21270115

The minimum control authority of a system of actuators with applications to Gravity Probe-B

NASA Technical Reports Server (NTRS)

Wiktor, Peter; Debra, Dan

1991-01-01

The forcing capabilities of systems composed of many actuators are analyzed in this paper. Multiactuator systems can generate higher forces in some directions than in others. Techniques are developed to find the force in the weakest direction. This corresponds to the worst-case output and is defined as the 'minimum control authority'. The minimum control authority is a function of three things: the actuator configuration, the actuator controller and the way in which the output of the system is limited. Three output limits are studied: (1) fuel-flow rate, (2) power, and (3) actuator output. The three corresponding actuator controllers are derived. These controllers generate the desired force while minimizing either fuel flow rate, power or actuator output. It is shown that using the optimal controller can substantially increase the minimum control authority. The techniques for calculating the minimum control authority are applied to the Gravity Probe-B spacecraft thruster system. This example shows that the minimum control authority can be used to design the individual actuators, choose actuator configuration, actuator controller, and study redundancy.

Detection of actinides and rare earths in natural matrices with the AGLAE new, high sensitivity detection set-up

NASA Astrophysics Data System (ADS)

Zucchiatti, Alessandro; Alonso, Ursula; Lemasson, Quentin; Missana, Tiziana; Moignard, Brice; Pacheco, Claire; Pichon, Laurent; Camarena de la Mora, Sandra

2014-08-01

A series of granite samples (Grimsel and Äspö) enriched by sorption with natU (10-3 M, 10-4 M, 10-5 M in solution) and La (10-3 M, 10-4 M in solution) has been scanned by PIXE over a surface of 1920 × 1920 mm2 together with non-enriched Grimsel and Äspö granites and a glass standard. An assessment of minimum detection limits, MDL's, for several elements has been performed with the use of standard materials. Due to mapping and the high sensitivity of the new AGLAE detection system, U levels around 30 ppm can be detected from the whole PIXE spectrum (one low energy detector and four summed filtered detectors) while U reach grains, inhomogeneously distributed over the surface can be clearly identified through the multi elemental maps and analyzed separately. Even the nominally enriched samples have La levels below the MDL, probably because precipitation of the element (and not adsorption) mostly took place, and precipitates were eliminated after surface cleaning carried out before PIXE analyses. A multi detector system that implies a PIXE detection solid angle much wider than in any other similar set-up (a factor of 2-5); a higher events selectivity, given by the possibility of filtering individually up to 4 PIXE detectors; a double RBS detector, the new Ion Beam Induced Luminescence (IBIL) spectrometry and gamma spectrometry. Full mapping capability in air, assisted by a powerful event by event reconstruction software. These features allow lower Minimum Detection Limits (MDL) which are highly beneficial to the analysis of cultural heritage objects, meaning generally a reduction of irradiation time. Paintings will then be studied without any damage to the pigments that have color change tendencies which is a major drawback of the previous system. Alternatively they could allow an increase in information collected at equal time, particularly considering the detector's fast response and therefore the potential for high beam currents when sample damage can be tolerated.This kind of set-up should be advantageous for the detection of elements that are present in a geological, archaeological or artistic samples to the level of a few tens ppm. This is true in particular for the rare earths which are relevant to the provenance attribution of various classes of cultural heritage objects (clays, glasses, …) and the actinides which are relevant in very specific and highly impacting dating problems and, more generally, critical environmental elements with special reference to the radionuclide mobility in deep geological formations hosting radioactive waste [2]. Geological materials are highly heterogeneous and consequently their retention of contaminants is heterogeneous as well. In this frame, the capabilities of the AGLAE set-up would allow an improved characterization of natural heterogeneous rock, detecting the presence of the elements of interest (actinides and rare earth) at concentration levels of tens of ppm. This provides a better definition of the initial system, avoiding biased interpretation of the retention properties of the material for the analysis of possible contamination. Additionally, if lower detection limits were achieved, new perspectives to evaluate retention of low solubility contaminants in a wider range of geochemical conditions would be opened.A glass standard and a series of reference granite samples (Grimsel and Äspö), either enriched by sorption with natU and La or kept natural, have been scanned by PIXE at the New-AGLAE detection system, to test measurement protocols and assess the MDL's allowed by the five detectors system.

2-µm wavelength-range low-loss inhibited-coupling hollow-core PCF

NASA Astrophysics Data System (ADS)

Maurel, M.; Chafer, M.; Delahaye, F.; Amrani, F.; Debord, B.; Gerome, F.; Benabid, F.

2018-02-01

We report on the design and fabrication of inhibited-coupling guiding hollow-core photonic crystal fiber with a transmission band optimized for low loss guidance around 2 μm. Two fibers design based on a Kagome-lattice cladding have been studied to demonstrate a minimum loss figure of 25 dB/km at 2 μm associated to an ultra-broad transmission band spanning from the visible to our detection limit of 3.4 μm. Such fibers could be an excellent tool to deliver and compress ultra-short pulse laser systems, especially for the emerging 2-3 μm spectral region.

Electrochemiluminescent DNA sensor based on controlled Zn-mediated grafting of diazonium precursors.

PubMed

Torréns, Mabel; Ortiz, Mayreli; Bejarano-Nosas, Diego; O'Sullivan, Ciara K

2015-07-01

Controlled Zn-mediated grafting of a thin layer of a diazonium salt was used to functionalise a carbon electrode with ruthenium(II)-tris-bipyridine (Ru)-labelled DNA for use as a capture probe in an electrochemiluminescent genosensor. A secondary reporter probe was labelled with a ferrocene (Fc) molecule, and in the presence of the single-stranded DNA target a genocomplex formed, where the Fc-label effectively quenched the electrochemiluminescence of the signal emitted from the Ru-label. The spacing of the labels for maximum sensitivity and minimum detection limit was optimised, and the signal reproducibility and stability of the method was established.

Ion-neutral Coupling During Deep Solar Minimum

NASA Technical Reports Server (NTRS)

Huang, Cheryl Y.; Roddy, Patrick A.; Sutton, Eric K.; Stoneback, Russell; Pfaff, Robert F.; Gentile, Louise C.; Delay, Susan H.

2013-01-01

The equatorial ionosphere under conditions of deep solar minimum exhibits structuring due to tidal forces. Data from instruments carried by the Communication Navigation Outage Forecasting System (CNOFS) which was launched in April 2008 have been analyzed for the first 2 years following launch. The Planar Langmuir Probe (PLP), Ion Velocity Meter (IVM) and Vector Electric Field Investigation (VEFI) all detect periodic structures during the 20082010 period which appear to be tides. However when the tidal features detected by these instruments are compared, there are distinctive and significant differences between the observations. Tides in neutral densities measured by the Gravity Recovery and Climate Experiment (GRACE) satellite were also observed during June 2008. In addition, Broad Plasma Decreases (BPDs) appear as a deep absolute minimum in the plasma and neutral density tidal pattern. These are co-located with regions of large downward-directed ion meridional velocities and minima in the zonal drifts, all on the nightside. The region in which BPDs occur coincides with a peak in occurrence rate of dawn depletions in plasma density observed on the Defense Meterological Satellite Program (DMSP) spacecraft, as well as a minimum in radiance detected by UV imagers on the Thermosphere Ionosphere Mesosphere Energetics and Dynamics (TIMED) and IMAGE satellites

Characterization of Terahertz Bi-Material Sensors with Integrated Metamaterial Absorbers

DTIC Science & Technology

2013-09-01

Kumar, Qing Hu, and J. L. Reno, “Real-time imaging using a 4.3-THz quantum cascade laser and a 320x240 microbolometer focal-plane array ,” IEEE...responsivity, the speed of operation and the minimum detected incident power were measured using a quantum cascade laser (QCL), operating at 3.8 THz...of operation and the minimum detected incident power were measured using a quantum cascade laser (QCL), operating at 3.8 THz. The measured

Minimum Error Bounded Efficient L1 Tracker with Occlusion Detection (PREPRINT)

DTIC Science & Technology

2011-01-01

Minimum Error Bounded Efficient `1 Tracker with Occlusion Detection Xue Mei\\ ∗ Haibin Ling† Yi Wu†[ Erik Blasch‡ Li Bai] \\Assembly Test Technology...proposed BPR-L1 tracker is tested on several challenging benchmark sequences involving chal- lenges such as occlusion and illumination changes. In all...point method de - pends on the value of the regularization parameter λ. In the experiments, we found that the total number of PCG is a few hundred. The

How Low Can You Go? The Importance of Quantifying Minimum Generation Levels for Renewable Integration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Denholm, Paul L; Brinkman, Gregory L; Mai, Trieu T

One of the significant limitations of solar and wind deployment is declining value caused by the limited correlation of renewable energy supply and electricity demand as well as limited flexibility of the power system. Limited flexibility can result from thermal and hydro plants that cannot turn off or reduce output due to technical or economic limits. These limits include the operating range of conventional thermal power plants, the need for process heat from combined heat and power plants, and restrictions on hydro unit operation. To appropriately analyze regional and national energy policies related to renewable deployment, these limits must bemore » accurately captured in grid planning models. In this work, we summarize data sources and methods for U.S. power plants that can be used to capture minimum generation levels in grid planning tools, such as production cost models. We also provide case studies for two locations in the U.S. (California and Texas) that demonstrate the sensitivity of variable generation (VG) curtailment to grid flexibility assumptions which shows the importance of analyzing (and documenting) minimum generation levels in studies of increased VG penetration.« less

Improvements of low-level radioxenon detection sensitivity by a state-of-the art coincidence setup.

PubMed

Cagniant, A; Le Petit, G; Gross, P; Douysset, G; Richard-Bressand, H; Fontaine, J-P

2014-05-01

The ability to quantify isotopic ratios of 135, 133 m, 133 and 131 m radioxenon is essential for the verification of the Comprehensive Nuclear-Test Ban Treaty (CTBT). In order to improve detection limits, CEA has developed a new on-site setup using photon/electron coincidence (Le Petit et al., 2013. J. Radioanal. Nucl. Chem., DOI : 10.1007/s 10697-013-2525-8.). Alternatively, the electron detection cell equipped with large silicon chips (PIPS) can be used with HPGe detector for laboratory analysis purpose. This setup allows the measurement of β/γ coincidences for the detection of (133)Xe and (135)Xe; and K-shell Conversion Electrons (K-CE)/X-ray coincidences for the detection of (131m)Xe, (133m)Xe and (133)Xe as well. Good energy resolution of 11 keV at 130 keV and low energy threshold of 29 keV for the electron detection were obtained. This provides direct discrimination between K-CE from (133)Xe, (133m)Xe and (131m)Xe. Estimation of Minimum Detectable Activity (MDA) for (131m)Xe is in the order of 1mBq over a 4 day measurement. An analysis of an environmental radioxenon sample using this method is shown. © 2013 The Authors. Published by Elsevier Ltd All rights reserved.

Physical requirements and milestones for the HIT-PoP Experiment

NASA Astrophysics Data System (ADS)

Jarboe, Thomas

2011-10-01

Recent success with HIT-SI demonstrates the viability of steady inductive helicity injection (SIHI) as a spheromak formation and sustainment method. Results include the sustainment of toroidal current of over 50 kA, up to 40 kA of plasma current that is separate from the injectors, toroidal flux up to 6 times the peak injected flux, and j/n > 1014Am. All were achieved with 10MW or less applied power. This paper explores the requirements for a confinement test of the concept using a larger proof of principle experiment. The confinement experiment must not exceed the beta limit, the drift parameter limit, or the wall loading limit, where the drift parameter is (drift of electrons relative to ions to produce current)/(ion thermal speed). It must also exceed a minimum j/n, a minimum n a, and a minimum electron temperature, where a is the minor radius. The drift parameter limit and beta limit appear to play defining roles in spheromak performance leading to a very favorable scaling of wall loading with size. The milestones sequence suggested is the following: 1. Startup at drift parameter and beta limit minimum density. 2. Raise current until j/n exceeds 10-14Am. 3. Raise the current and temperature until T ~ 50 eV for good ionization. 4. Raise the current and density until n a > 2x1019 m-2 for neutral screening. 5. Raise current and temperature until T > 200eV so magnetic confinement can be studied.

Simulation study of melanoma detection in human skin tissues by laser-generated surface acoustic waves

NASA Astrophysics Data System (ADS)

Chen, Kun; Fu, Xing; Dorantes-Gonzalez, Dante J.; Lu, Zimo; Li, Tingting; Li, Yanning; Wu, Sen; Hu, Xiaotang

2014-07-01

Air pollution has been correlated to an increasing number of cases of human skin diseases in recent years. However, the investigation of human skin tissues has received only limited attention, to the point that there are not yet satisfactory modern detection technologies to accurately, noninvasively, and rapidly diagnose human skin at epidermis and dermis levels. In order to detect and analyze severe skin diseases such as melanoma, a finite element method (FEM) simulation study of the application of the laser-generated surface acoustic wave (LSAW) technique is developed. A three-layer human skin model is built, where LSAW's are generated and propagated, and their effects in the skin medium with melanoma are analyzed. Frequency domain analysis is used as a main tool to investigate such issues as minimum detectable size of melanoma, filtering spectra from noise and from computational irregularities, as well as on how the FEM model meshing size and computational capabilities influence the accuracy of the results. Based on the aforementioned aspects, the analysis of the signals under the scrutiny of the phase velocity dispersion curve is verified to be a reliable, a sensitive, and a promising approach for detecting and characterizing melanoma in human skin.

Simulation study of melanoma detection in human skin tissues by laser-generated surface acoustic waves.

PubMed

Chen, Kun; Fu, Xing; Dorantes-Gonzalez, Dante J; Lu, Zimo; Li, Tingting; Li, Yanning; Wu, Sen; Hu, Xiaotang

2014-01-01

Air pollution has been correlated to an increasing number of cases of human skin diseases in recent years. However, the investigation of human skin tissues has received only limited attention, to the point that there are not yet satisfactory modern detection technologies to accurately, noninvasively, and rapidly diagnose human skin at epidermis and dermis levels. In order to detect and analyze severe skin diseases such as melanoma, a finite element method (FEM) simulation study of the application of the laser-generated surface acoustic wave (LSAW) technique is developed. A three-layer human skin model is built, where LSAW’s are generated and propagated, and their effects in the skin medium with melanoma are analyzed. Frequency domain analysis is used as a main tool to investigate such issues as minimum detectable size of melanoma, filtering spectra from noise and from computational irregularities, as well as on how the FEM model meshing size and computational capabilities influence the accuracy of the results. Based on the aforementioned aspects, the analysis of the signals under the scrutiny of the phase velocity dispersion curve is verified to be a reliable, a sensitive, and a promising approach for detecting and characterizing melanoma in human skin.

Peptide-activated gold nanoparticles for selective visual sensing of virus

NASA Astrophysics Data System (ADS)

Sajjanar, Basavaraj; Kakodia, Bhuvna; Bisht, Deepika; Saxena, Shikha; Singh, Arvind Kumar; Joshi, Vinay; Tiwari, Ashok Kumar; Kumar, Satish

2015-05-01

In this study, we report peptide-gold nanoparticles (AuNP)-based visual sensor for viruses. Citrate-stabilized AuNP (20 ± 1.9 nm) were functionalized with strong sulfur-gold interface using cysteinylated virus-specific peptide. Peptide-Cys-AuNP formed complexes with the viruses which made them to aggregate. The aggregation can be observed with naked eye and also with UV-Vis spectrophotometer as a color change from bright red to purple. The test allows for fast and selective detection of specific viruses. Spectroscopic measurements showed high linear correlation ( R 2 = 0.995) between the changes in optical density ratio (OD610/OD520) with the different concentrations of virus. The new method was compared with the hemagglutinating (HA) test for Newcastle disease virus (NDV). The results indicated that peptide-Cys-AuNP was more sensitive and can visually detect minimum number of virus particles present in the biological samples. The limit of detection for the NDV was 0.125 HA units of the virus. The method allows for selective detection and quantification of the NDV, and requires no isolation of viral RNA and PCR experiments. This strategy may be utilized for detection of other important human and animal viral pathogens.

Minimum requirements for adequate nighttime conspicuity of highway signs

DOT National Transportation Integrated Search

1988-02-01

A laboratory and field study were conducted to assess the minimum luminance levels of signs to ensure that they will be detected and identified at adequate distances under nighttime driving conditions. A total of 30 subjects participated in the field...

25 CFR 542.42 - What are the minimum internal control standards for internal audit for Tier C gaming operations?

Code of Federal Regulations, 2010 CFR

2010-04-01

... reconciliation process; (ii) Pull tabs, including but not limited to, statistical records, winner verification... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum internal control standards for... COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM INTERNAL CONTROL STANDARDS § 542.42 What are...

Nonuniform distribution of phase noise in distributed acoustic sensing based on phase-sensitive OTDR

NASA Astrophysics Data System (ADS)

Yu, Zhijie; Lu, Yang; Meng, Zhou

2017-10-01

A phase-sensitive optical time-domain reflectometry (∅-OTDR) implements distributed acoustic sensing (DAS) due to its ability for high sensitivity vibration measurement. Phase information of acoustic vibration events can be acquired by interrogation of the vibration-induced phase change between coherent Rayleigh scattering light from two points of the sensing fiber. And DAS can be realized when applying phase generated carrier (PGC) algorithm to the whole sensing fiber while the sensing fiber is transformed into a series of virtual sensing channels. Minimum detectable vibration of a ∅-OTDR is limited by phase noise level. In this paper, nonuniform distribution of phase noise of virtual sensing channels in a ∅-OTDR is investigated theoretically and experimentally. Correspondence between the intensity of Rayleigh scattering light and interference fading as well as polarization fading is analyzed considering inner interference of coherent Rayleigh light scattered from a multitude of scatters within pulse duration, and intensity noise related to the intensity of Rayleigh scattering light can be converted to phase noise while measuring vibration-induced phase change. Experiments are performed and the results confirm the predictions of the theoretical analysis. This study is essential for acquiring insight into nonuniformity of phase noise in DAS based on a ∅-OTDR, and would put forward some feasible methods to eliminate the effect of interference fading and polarization fading and optimize the minimum detectable vibration of a ∅-OTDR.

Estimating occupancy and predicting numbers of gray wolf packs in Montana using hunter surveys

USGS Publications Warehouse

Rich, Lindsey N.; Russell, Robin E.; Glenn, Elizabeth M.; Mitchell, Michael S.; Gude, Justin A.; Podruzny, Kevin M.; Sime, Carolyn A.; Laudon, Kent; Ausband, David E.; Nichols, James D.

2013-01-01

Reliable knowledge of the status and trend of carnivore populations is critical to their conservation and management. Methods for monitoring carnivores, however, are challenging to conduct across large spatial scales. In the Northern Rocky Mountains, wildlife managers need a time- and cost-efficient method for monitoring gray wolf (Canis lupus) populations. Montana Fish, Wildlife and Parks (MFWP) conducts annual telephone surveys of >50,000 deer and elk hunters. We explored how survey data on hunters' sightings of wolves could be used to estimate the occupancy and distribution of wolf packs and predict their abundance in Montana for 2007–2009. We assessed model utility by comparing our predictions to MFWP minimum known number of wolf packs. We minimized false positive detections by identifying a patch as occupied if 2–25 wolves were detected by ≥3 hunters. Overall, estimates of the occupancy and distribution of wolf packs were generally consistent with known distributions. Our predictions of the total area occupied increased from 2007 to 2009 and predicted numbers of wolf packs were approximately 1.34–1.46 times the MFWP minimum counts for each year of the survey. Our results indicate that multi-season occupancy models based on public sightings can be used to monitor populations and changes in the spatial distribution of territorial carnivores across large areas where alternative methods may be limited by personnel, time, accessibility, and budget constraints.

Frost damage in citric and olive production as the result of climate degradation

NASA Astrophysics Data System (ADS)

Saa Requejo, A.; Díaz Alvarez, M. C.; Tarquis, A. M.; Burgaz Moreno, F.; Garcia Moreno, R.

2009-04-01

Low temperature is one of the chief limiting factors in plant distribution. Freezing temperature shortens the growing season and may lower the yield and quality of any number of fruit crops. Minimum temperatures records for the Spanish region of Murcia were studied as limiting factor in fruit production. An analysis of temperature series since 1935 showed that the range of the absolute minimum temperatures (Tmin) on frost days in the target year, namely -0.5 °C to -4.0°C, was statistically similar to the range recorded in 1993, while the mean minimum temperatures (tmin) were found to have risen. The historical series also showed the mean minimum temperatures (tmin) to have increased, however. Through 1985, tmin ranged from 4.0 to -2.0 °C, depending on the area, while these limits shifted in more recent years to 7.0 - 0.5 °C. This increase in mean temperature produced that the frost episodes in March 2004 was considered by lemon, mandarin and olive producers as the worst in many years for frost damage since the minimum temperature was reached in a more sensitive phenological stage, despite the statistical evidence that similar freezing temperatures had been reached on similar dates in other years.

Tritium concentrations in flow from selected springs that discharge to the Snake River, Twin Falls-Hagerman area, Idaho

USGS Publications Warehouse

Mann, L.J.

1989-01-01

Concern has been expressed that some of the approximately 30,900 curies of tritium disposed to the Snake River Plain aquifer from 1952 to 1988 at the INEL (Idaho National Engineering Laboratory) have migrated to springs discharging to the Snake River in the Twin Falls-Hagerman area. To document tritium concentrations in springflow, 17 springs were sampled in November 1988 and 19 springs were sampled in March 1989. Tritium concentrations were less than the minimum detectable concentration of 0.5 pCi/mL (picocuries/mL) in November 1988 and less than the minimum detectable concentration of 0.2 pCi/mL in March 1989; the minimum detectable concentration was smaller in March 1989 owing to a longer counting time in the liquid scintillation system. The maximum contaminant level of tritium in drinking water as established by the U.S. Environmental Protection Agency is 20 pCi/mL. U.S. Environmental Protection Agency sample analyses indicate that the tritium concentration has decreased in the Snake River near Buhl since the 1970's. In 1974-79, tritium concentrations were less than 0.3 +/-0.2 pCi/mL in 3 of 20 samples; in 1983-88, 17 of 23 samples contained less than 0.3 +/-0.2 pCi/mL of tritium; the minimum detectable concentration is 0.2 pCi/mL. On the basis of decreasing tritium concentrations in the Snake River, their correlation to cessation of atmospheric weapons tests tritium concentrations in springflow less than the minimum detectable concentration, and the distribution of tritium in groundwater at the INEL, aqueous disposal of tritium at the INEL has had no measurable effect on tritium concentrations in springflow from the Snake River Plain aquifer and in the Snake River near Buhl. (USGS)

Indirect detection constraints on s- and t-channel simplified models of dark matter

NASA Astrophysics Data System (ADS)

Carpenter, Linda M.; Colburn, Russell; Goodman, Jessica; Linden, Tim

2016-09-01

Recent Fermi-LAT observations of dwarf spheroidal galaxies in the Milky Way have placed strong limits on the gamma-ray flux from dark matter annihilation. In order to produce the strongest limit on the dark matter annihilation cross section, the observations of each dwarf galaxy have typically been "stacked" in a joint-likelihood analysis, utilizing optical observations to constrain the dark matter density profile in each dwarf. These limits have typically been computed only for singular annihilation final states, such as b b ¯ or τ+τ- . In this paper, we generalize this approach by producing an independent joint-likelihood analysis to set constraints on models where the dark matter particle annihilates to multiple final-state fermions. We interpret these results in the context of the most popular simplified models, including those with s- and t-channel dark matter annihilation through scalar and vector mediators. We present our results as constraints on the minimum dark matter mass and the mediator sector parameters. Additionally, we compare our simplified model results to those of effective field theory contact interactions in the high-mass limit.

Absorption measurements of the second overtone band of NO in ambient and combustion gases with a 1.8-mum room-temperature diode laser.

PubMed

Sonnenfroh, D M; Allen, M G

1997-10-20

We describe the development of a room-temperature diode sensor for in situ monitoring of combustion-generated NO. The sensor is based on a near-IR diode laser operating near 1.8 mum, which probes isolated transitions in the second overtone (3, 0) absorption band of NO. Based on absorption cell data, the sensitivity for ambient atmospheric pressure conditions is of the order of 30 parts in 10(6) by volume for a meter path (ppmv-m), assuming a minimum measurable absorbance of 10(-5). Initial H(2) -air flame measurements are complicated by strong water vapor absorption features that constrain the available gain and dynamic range of the present detection system. Preliminary results suggest that detection limits in this environment of the order of 140 ppmv-m could be achieved with optimum baseline correction.

Absorption measurements of the second overtone band of NO in ambient and combustion gases with a 1.8- m room-temperature diode laser

NASA Astrophysics Data System (ADS)

Sonnenfroh, David M.; Allen, Mark G.

1997-10-01

We describe the development of a room-temperature diode sensor for in situ monitoring of combustion-generated NO. The sensor is based on a near-IR diode laser operating near 1.8 m, which probes isolated transitions in the second overtone (3,0) absorption band of NO. Based on absorption cell data, the sensitivity for ambient atmospheric pressure conditions is of the order of 30 parts in 10 6 by volume for a meter path (ppmv m), assuming a minimum measurable absorbance of 10 5 . Initial H 2 air flame measurements are complicated by strong water vapor absorption features that constrain the available gain and dynamic range of the present detection system. Preliminary results suggest that detection limits in this environment of the order of 140 ppmv m could be achieved with optimum baseline correction.

Carboxylated multiwalled carbon nanotubes/polydimethylsiloxane, a new coating for 96-blade solid-phase microextraction for determination of phenolic compounds in water.

PubMed

Kueseng, Pamornrat; Pawliszyn, Janusz

2013-11-22

A new thin-film, carboxylated multiwalled carbon nanotubes/polydimethylsiloxane (MWCNTs-COOH/PDMS) coating was developed for 96-blade solid-phase microextraction (SPME) system followed by high performance liquid chromatography with ultraviolet detection (HPLC-UV). The method provided good extraction efficiency (64-90%) for three spiked levels, with relative standard deviations (RSD)≤6%, and detection limits between 1 and 2 μg/L for three phenolic compounds. The MWCNTs-COOH/PDMS 96-blade SPME system presents advantages over traditional methods due to its simplicity of use, easy coating preparation, low cost and high sample throughput (2.1 min per sample). The developed coating is reusable for a minimum of 110 extractions with good extraction efficiency. The coating provided higher extraction efficiency (3-8 times greater) than pure PDMS coatings. Copyright © 2013 Elsevier B.V. All rights reserved.

The study of in vivo quantification of aluminum (Al) in human bone with a compact DD generator-based neutron activation analysis (NAA) system.

PubMed

Byrne, Patrick; Mostafaei, Farshad; Liu, Yingzi; Blake, Scott P; Koltick, David; Nie, Linda H

2016-05-01

The feasibility and methodology of using a compact DD generator-based neutron activation analysis system to measure aluminum in hand bone has been investigated. Monte Carlo simulations were used to simulate the moderator, reflector, and shielding assembly and to estimate the radiation dose. A high purity germanium (HPGe) detector was used to detect the Al gamma ray signals. The minimum detectable limit (MDL) was found to be 11.13 μg g(-1) dry bone (ppm). An additional HPGe detector would improve the MDL by a factor of 1.4, to 7.9 ppm. The equivalent dose delivered to the irradiated hand was calculated by Monte Carlo to be 11.9 mSv. In vivo bone aluminum measurement with the DD generator was found to be feasible among general population with an acceptable dose to the subject.

Performance measures for lower gastrointestinal endoscopy: a European Society of Gastrointestinal Endoscopy (ESGE) quality improvement initiative

PubMed Central

Thomas-Gibson, Siwan; Bugajski, Marek; Bretthauer, Michael; Rees, Colin J; Dekker, Evelien; Hoff, Geir; Jover, Rodrigo; Suchanek, Stepan; Ferlitsch, Monika; Anderson, John; Roesch, Thomas; Hultcranz, Rolf; Racz, Istvan; Kuipers, Ernst J; Garborg, Kjetil; East, James E; Rupinski, Maciej; Seip, Birgitte; Bennett, Cathy; Senore, Carlo; Minozzi, Silvia; Bisschops, Raf; Domagk, Dirk; Valori, Roland; Spada, Cristiano; Hassan, Cesare; Dinis-Ribeiro, Mario; Rutter, Matthew D

2017-01-01

The European Society of Gastrointestinal Endoscopy and United European Gastroenterology present a short list of key performance measures for lower gastrointestinal endoscopy. We recommend that endoscopy services across Europe adopt the following seven key performance measures for lower gastrointestinal endoscopy for measurement and evaluation in daily practice at a center and endoscopist level: 1 rate of adequate bowel preparation (minimum standard 90%); 2 cecal intubation rate (minimum standard 90%); 3 adenoma detection rate (minimum standard 25%); 4 appropriate polypectomy technique (minimum standard 80%); 5 complication rate (minimum standard not set); 6 patient experience (minimum standard not set); 7 appropriate post-polypectomy surveillance recommendations (minimum standard not set). Other identified performance measures have been listed as less relevant based on an assessment of their importance, scientific acceptability, feasibility, usability, and comparison to competing measures. PMID:28507745

Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis.

PubMed

Choi, Yeonim; Jeon, Bo-Young; Shim, Tae Sun; Jin, Hyunwoo; Cho, Sang-Nae; Lee, Hyeyoung

2014-12-01

Rapid, accurate detection of Mycobacterium tuberculosis is crucial in the diagnosis of tuberculosis (TB), but conventional diagnostic methods have limited sensitivity and specificity or are time consuming. A new highly sensitive nucleic acid amplification test, combined nested and real-time polymerase chain reaction (PCR) in a single tube (one-tube nested real-time PCR), was developed for detecting M. tuberculosis, which takes advantage of two PCR techniques, i.e., nested PCR and real-time PCR. One-tube nested real-time PCR was designed to have two sequential reactions with two sets of primers and dual probes for the insertion sequence (IS) 6110 sequence of M. tuberculosis in a single closed tube. The minimum limits of detection of IS6110 real-time PCR and IS6110 one-tube nested real-time PCR were 100 fg/μL and 1 fg/μL of M. tuberculosis DNA, respectively. AdvanSure TB/non-tuberculous mycobacteria (NTM) real-time PCR, IS6110 real-time PCR, and two-tube nested real-time PCR showed 100% sensitivity and 100% specificity for clinical M. tuberculosis isolates and NTM isolates. In comparison, the sensitivities of AdvanSure TB/NTM real-time PCR, single IS6110 real-time PCR, and one-tube nested real-time PCR were 91% (152/167), 94.6% (158/167), and 100% (167/167) for sputum specimens, respectively. In conclusion, IS6110 one-tube nested real-time PCR is useful for detecting M. tuberculosis due to its high sensitivity and simple manipulation. Copyright © 2014 Elsevier Inc. All rights reserved.

Analysis of chemical warfare agents in food products by atmospheric pressure ionization-high field asymmetric waveform ion mobility spectrometry-mass spectrometry.

PubMed

Kolakowski, Beata M; D'Agostino, Paul A; Chenier, Claude; Mester, Zoltán

2007-11-01

Flow injection high field asymmetric waveform ion mobility spectrometry (FAIMS)-mass spectrometry (MS) methodology was developed for the detection and identification of chemical warfare (CW) agents in spiked food products. The CW agents, soman (GD), sarin (GB), tabun (GA), cyclohexyl sarin (GF), and four hydrolysis products, ethylphosphonic acid (EPA), methylphosphonic acid (MPA), pinacolyl methylphosphonic acid (Pin MPA), and isopropyl methylphosphonic acid (IMPA) were separated and detected by positive ion and negative ion atmospheric pressure ionization-FAIMS-MS. Under optimized conditions, the compensation voltages were 7.2 V for GD, 8.0 V for GA, 7.2 V for GF, 7.6 V for GB, 18.2 V for EPA, 25.9 V for MPA, -1.9 V for PinMPA, and +6.8 V for IMPA. Sample preparation was kept to a minimum, resulting in analysis times of 3 min or less per sample. The developed methodology was evaluated by spiking bottled water, canola oil, cornmeal, and honey samples at low microgram per gram (or microg/mL) levels with the CW agents or CW agent hydrolysis products. The detection limits observed for the CW agents in the spiked food samples ranged from 3 to 15 ng/mL in bottled water, 1-33 ng/mL in canola oil, 1-34 ng/g in cornmeal, and 13-18 ng/g in honey. Detection limits were much higher for the CW agent hydrolysis products, with only MPA being detected in spiked honey samples.

The effect of warmer winters on the demography of an outbreak insect is hidden by intraspecific competition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goodsman, Devin W.; Grosklos, Guenchik; Aukema, Brian H.

Warmer climates are predicted to increase bark beetle outbreak frequency, severity, and range. Even in favorable climates, however, outbreaks can decelerate due to resource limitation, which necessitates the inclusion of competition for limited resources in analyses of climatic effects on populations. We evaluated several hypotheses of how climate impacts mountain pine beetle reproduction using an extensive 9-year dataset, in which nearly 10,000 trees were sampled across a region of approximately 90,000 km 2, that was recently invaded by the mountain pine beetle in Alberta, Canada. Our analysis supports the hypothesis of a positive effect of warmer winter temperatures on mountainmore » pine beetle overwinter survival and provides evidence that the increasing trend in minimum winter temperatures over time in North America is an important driver of increased mountain pine beetle reproduction across the region. Although we demonstrate a consistent effect of warmer minimum winter temperatures on mountain pine beetle reproductive rates that is evident at the landscape and regional scales, this effect is overwhelmed by the effect of competition for resources within trees at the site level. Our results suggest that detection of the effects of a warming climate on bark beetle populations at small spatial scales may be difficult without accounting for negative density dependence due to competition for resources.« less

The effect of warmer winters on the demography of an outbreak insect is hidden by intraspecific competition.

PubMed

Goodsman, Devin W; Grosklos, Guenchik; Aukema, Brian H; Whitehouse, Caroline; Bleiker, Katherine P; McDowell, Nate G; Middleton, Richard S; Xu, Chonggang

2018-05-29

Warmer climates are predicted to increase bark beetle outbreak frequency, severity, and range. Even in favorable climates, however, outbreaks can decelerate due to resource limitation, which necessitates the inclusion of competition for limited resources in analyses of climatic effects on populations. We evaluated several hypotheses of how climate impacts mountain pine beetle reproduction using an extensive 9-year dataset, in which nearly 10,000 trees were sampled across a region of approximately 90,000 km 2 , that was recently invaded by the mountain pine beetle in Alberta, Canada. Our analysis supports the hypothesis of a positive effect of warmer winter temperatures on mountain pine beetle overwinter survival and provides evidence that the increasing trend in minimum winter temperatures over time in North America is an important driver of increased mountain pine beetle reproduction across the region. Although we demonstrate a consistent effect of warmer minimum winter temperatures on mountain pine beetle reproductive rates that is evident at the landscape and regional scales, this effect is overwhelmed by the effect of competition for resources within trees at the site level. Our results suggest that detection of the effects of a warming climate on bark beetle populations at small spatial scales may be difficult without accounting for negative density dependence due to competition for resources. © 2018 The Authors. Global Change Biology Published by John Wiley & Sons Ltd.

The effect of warmer winters on the demography of an outbreak insect is hidden by intraspecific competition

DOE PAGES

Goodsman, Devin W.; Grosklos, Guenchik; Aukema, Brian H.; ...

2018-05-29

Warmer climates are predicted to increase bark beetle outbreak frequency, severity, and range. Even in favorable climates, however, outbreaks can decelerate due to resource limitation, which necessitates the inclusion of competition for limited resources in analyses of climatic effects on populations. We evaluated several hypotheses of how climate impacts mountain pine beetle reproduction using an extensive 9-year dataset, in which nearly 10,000 trees were sampled across a region of approximately 90,000 km 2, that was recently invaded by the mountain pine beetle in Alberta, Canada. Our analysis supports the hypothesis of a positive effect of warmer winter temperatures on mountainmore » pine beetle overwinter survival and provides evidence that the increasing trend in minimum winter temperatures over time in North America is an important driver of increased mountain pine beetle reproduction across the region. Although we demonstrate a consistent effect of warmer minimum winter temperatures on mountain pine beetle reproductive rates that is evident at the landscape and regional scales, this effect is overwhelmed by the effect of competition for resources within trees at the site level. Our results suggest that detection of the effects of a warming climate on bark beetle populations at small spatial scales may be difficult without accounting for negative density dependence due to competition for resources.« less

A Simple and Rapid Identification Method for Mycobacterium bovis BCG with Loop-Mediated Isothermal Amplification.

PubMed

Kouzaki, Yuji; Maeda, Takuya; Sasaki, Hiroaki; Tamura, Shinsuke; Hamamoto, Takaaki; Yuki, Atsushi; Sato, Akinori; Miyahira, Yasushi; Kawana, Akihiko

2015-01-01

Bacillus Calmette-Guérin (BCG) is widely used as a live attenuated vaccine against Mycobacterium tuberculosis and is an agent for standard prophylaxis against the recurrence of bladder cancer. Unfortunately, it can cause severe infectious diseases, especially in immunocompromised patients, and the ability to immediately distinguish BCG from other M. tuberculosis complexes is therefore important. In this study, we developed a simple and easy-to-perform identification procedure using loop-mediated amplification (LAMP) to detect deletions within the region of difference, which is deleted specifically in all M. bovis BCG strains. Reactions were performed at 64 °C for 30 min and successful targeted gene amplifications were detected by real-time turbidity using a turbidimeter and visual inspection of color change. The assay had an equivalent detection limit of 1.0 pg of genomic DNA using a turbidimeter whereas it was 10 pg with visual inspection, and it showed specificity against 49 strains of 44 pathogens, including M. tuberculosis complex. The expected LAMP products were confirmed through identical melting curves in real-time LAMP procedures. We employed the Procedure for Ultra Rapid Extraction (PURE) kit to isolate mycobacterial DNA and found that the highest sensitivity limit with a minimum total cell count of mycobacterium (including DNA purification with PURE) was up to 1 × 10(3) cells/reaction, based on color changes under natural light with FDA reagents. The detection limit of this procedure when applied to artificial serum, urine, cerebrospinal fluid, and bronchoalveolar lavage fluid samples was also about 1 × 10(3) cells/reaction. Therefore, this substitute method using conventional culture or clinical specimens followed by LAMP combined with PURE could be a powerful tool to enable the rapid identification of M. bovis BCG as point-of-care testing. It is suitable for practical use not only in resource-limited situations, but also in any clinical situation involving immunocompromised patients because of its convenience, rapidity, and cost effectiveness.

A Simple and Rapid Identification Method for Mycobacterium bovis BCG with Loop-Mediated Isothermal Amplification

PubMed Central

Kouzaki, Yuji; Maeda, Takuya; Sasaki, Hiroaki; Tamura, Shinsuke; Hamamoto, Takaaki; Yuki, Atsushi; Sato, Akinori; Miyahira, Yasushi; Kawana, Akihiko

2015-01-01

Bacillus Calmette-Guérin (BCG) is widely used as a live attenuated vaccine against Mycobacterium tuberculosis and is an agent for standard prophylaxis against the recurrence of bladder cancer. Unfortunately, it can cause severe infectious diseases, especially in immunocompromised patients, and the ability to immediately distinguish BCG from other M. tuberculosis complexes is therefore important. In this study, we developed a simple and easy-to-perform identification procedure using loop-mediated amplification (LAMP) to detect deletions within the region of difference, which is deleted specifically in all M. bovis BCG strains. Reactions were performed at 64°C for 30 min and successful targeted gene amplifications were detected by real-time turbidity using a turbidimeter and visual inspection of color change. The assay had an equivalent detection limit of 1.0 pg of genomic DNA using a turbidimeter whereas it was 10 pg with visual inspection, and it showed specificity against 49 strains of 44 pathogens, including M. tuberculosis complex. The expected LAMP products were confirmed through identical melting curves in real-time LAMP procedures. We employed the Procedure for Ultra Rapid Extraction (PURE) kit to isolate mycobacterial DNA and found that the highest sensitivity limit with a minimum total cell count of mycobacterium (including DNA purification with PURE) was up to 1 × 103 cells/reaction, based on color changes under natural light with FDA reagents. The detection limit of this procedure when applied to artificial serum, urine, cerebrospinal fluid, and bronchoalveolar lavage fluid samples was also about 1 × 103 cells/reaction. Therefore, this substitute method using conventional culture or clinical specimens followed by LAMP combined with PURE could be a powerful tool to enable the rapid identification of M. bovis BCG as point-of-care testing. It is suitable for practical use not only in resource-limited situations, but also in any clinical situation involving immunocompromised patients because of its convenience, rapidity, and cost effectiveness. PMID:26208001

25 CFR 542.22 - What are the minimum internal control standards for internal audit for Tier A gaming operations?

Code of Federal Regulations, 2010 CFR

2010-04-01

... but not limited to, bingo card control, payout procedures, and cash reconciliation process; (ii) Pull... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum internal control standards for... COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM INTERNAL CONTROL STANDARDS § 542.22 What are...

25 CFR 542.32 - What are the minimum internal control standards for internal audit for Tier B gaming operations?

Code of Federal Regulations, 2010 CFR

2010-04-01

... but not limited to, bingo card control, payout procedures, and cash reconciliation process; (ii) Pull... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum internal control standards for... COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM INTERNAL CONTROL STANDARDS § 542.32 What are...

The Age 21 Minimum Legal Drinking Age Law. Prevention Update

ERIC Educational Resources Information Center

Higher Education Center for Alcohol, Drug Abuse, and Violence Prevention, 2011

2011-01-01

Currently, all 50 states limit alcohol purchases to people aged 21 and over. But that hasn't always been the case. In fact, it was July 17, 1984, when President Ronald Reagan signed the national 21 minimum legal drinking age (MLDA) legislation into law. At that time, only 23 states had minimum alcohol purchasing ages of 21 years old. The…

Nearest Neighbor Averaging and its Effect on the Critical Level and Minimum Detectable Concentration for Scanning Radiological Survey Instruments that Perform Facility Release Surveys.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fournier, Sean Donovan; Beall, Patrick S; Miller, Mark L

2014-08-01

Through the SNL New Mexico Small Business Assistance (NMSBA) program, several Sandia engineers worked with the Environmental Restoration Group (ERG) Inc. to verify and validate a novel algorithm used to determine the scanning Critical Level (L c ) and Minimum Detectable Concentration (MDC) (or Minimum Detectable Areal Activity) for the 102F scanning system. Through the use of Monte Carlo statistical simulations the algorithm mathematically demonstrates accuracy in determining the L c and MDC when a nearest-neighbor averaging (NNA) technique was used. To empirically validate this approach, SNL prepared several spiked sources and ran a test with the ERG 102F instrumentmore » on a bare concrete floor known to have no radiological contamination other than background naturally occurring radioactive material (NORM). The tests conclude that the NNA technique increases the sensitivity (decreases the L c and MDC) for high-density data maps that are obtained by scanning radiological survey instruments.« less

EARLY SCIENCE WITH THE KOREAN VLBI NETWORK: THE QCAL-1 43 GHz CALIBRATOR SURVEY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Petrov, Leonid; Lee, Sang-Sung; Kim, Jongsoo

2012-11-01

This paper presents the catalog of correlated flux densities in three ranges of baseline projection lengths of 637 sources from a 43 GHz (Q band) survey observed with the Korean VLBI Network. Of them, 14 objects used as calibrators were previously observed, but 623 sources have not been observed before in the Q band with very long baseline interferometry (VLBI). The goal of this work in the early science phase of the new VLBI array is twofold: to evaluate the performance of the new instrument that operates in a frequency range of 22-129 GHz and to build a list ofmore » objects that can be used as targets and as calibrators. We have observed the list of 799 target sources with declinations down to -40 Degree-Sign . Among them, 724 were observed before with VLBI at 22 GHz and had correlated flux densities greater than 200 mJy. The overall detection rate is 78%. The detection limit, defined as the minimum flux density for a source to be detected with 90% probability in a single observation, was in the range of 115-180 mJy depending on declination. However, some sources as weak as 70 mJy have been detected. Of 623 detected sources, 33 objects are detected for the first time in VLBI mode. We determined their coordinates with a median formal uncertainty of 20 mas. The results of this work set the basis for future efforts to build the complete flux-limited sample of extragalactic sources at frequencies of 22 GHz and higher at 3/4 of the celestial sphere.« less

Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia

PubMed Central

Pérez-Castro, Rosalía; Castellanos, Jaime E; Olano, Víctor A; Matiz, María Inés; Jaramillo, Juan F; Vargas, Sandra L; Sarmiento, Diana M; Stenström, Thor Axel; Overgaard, Hans J

2016-01-01

The Aedes aegypti vector for dengue virus (DENV) has been reported in urban and periurban areas. The information about DENV circulation in mosquitoes in Colombian rural areas is limited, so we aimed to evaluate the presence of DENV in Ae. aegypti females caught in rural locations of two Colombian municipalities, Anapoima and La Mesa. Mosquitoes from 497 rural households in 44 different rural settlements were collected. Pools of about 20 Ae. aegypti females were processed for DENV serotype detection. DENV in mosquitoes was detected in 74% of the analysed settlements with a pool positivity rate of 62%. The estimated individual mosquito infection rate was 4.12% and the minimum infection rate was 33.3/1,000 mosquitoes. All four serotypes were detected; the most frequent being DENV-2 (50%) and DENV-1 (35%). Two-three serotypes were detected simultaneously in separate pools. This is the first report on the co-occurrence of natural DENV infection of mosquitoes in Colombian rural areas. The findings are important for understanding dengue transmission and planning control strategies. A potential latent virus reservoir in rural areas could spill over to urban areas during population movements. Detecting DENV in wild-caught adult mosquitoes should be included in the development of dengue epidemic forecasting models. PMID:27074252

A prototype detection system for atmospheric monitoring of xenon radioisotopes

NASA Astrophysics Data System (ADS)

Czyz, Steven A.; Farsoni, Abi T.; Ranjbar, Lily

2018-03-01

The design of a radioxenon detection system utilizing a CdZeTe crystal and a plastic scintillator coupled to an array of SiPMs to conduct beta-gamma coincidence detection for atmospheric radioxenon monitoring, as well as the measurement of 135Xe and 133/133mXe, have been detailed previously. This paper presents recent measurements of 133/133mXe and 131mXe and the observation of conversion electrons in their coincidence spectra, as well as a 48-hour background measurement to calculate the Minimum Detectable Concentration (MDC) of radioxenon isotopes in the system. The identification of Regions of Interest (ROIs) in the coincidence spectra yielded from the radioxenon measurements, and the subsequent calculation of the MDCs of the system for 135Xe, 133/133mXe, and 131mXe, are also discussed. Calculated MDCs show that the detection system preforms respectably when compared to other state of the art radioxenon detection systems and achieved an MDC of less than 1 mBq/m3 for 131mXe, 133Xe, and 133mXe, in accordance with limits set by the Comprehensive Nuclear-Test-Ban Treaty (CTBTO). The system also provides the advantage of room temperature operation, compactness, low noise operation and having simple readout electronics.

Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia.

PubMed

Pérez-Castro, Rosalía; Castellanos, Jaime E; Olano, Víctor A; Matiz, María Inés; Jaramillo, Juan F; Vargas, Sandra L; Sarmiento, Diana M; Stenström, Thor Axel; Overgaard, Hans J

2016-04-01

The Aedes aegypti vector for dengue virus (DENV) has been reported in urban and periurban areas. The information about DENV circulation in mosquitoes in Colombian rural areas is limited, so we aimed to evaluate the presence of DENV in Ae. aegypti females caught in rural locations of two Colombian municipalities, Anapoima and La Mesa. Mosquitoes from 497 rural households in 44 different rural settlements were collected. Pools of about 20 Ae. aegypti females were processed for DENV serotype detection. DENV in mosquitoes was detected in 74% of the analysed settlements with a pool positivity rate of 62%. The estimated individual mosquito infection rate was 4.12% and the minimum infection rate was 33.3/1,000 mosquitoes. All four serotypes were detected; the most frequent being DENV-2 (50%) and DENV-1 (35%). Two-three serotypes were detected simultaneously in separate pools. This is the first report on the co-occurrence of natural DENV infection of mosquitoes in Colombian rural areas. The findings are important for understanding dengue transmission and planning control strategies. A potential latent virus reservoir in rural areas could spill over to urban areas during population movements. Detecting DENV in wild-caught adult mosquitoes should be included in the development of dengue epidemic forecasting models.

Colorimetric Method of Loop-Mediated Isothermal Amplification with the Pre-Addition of Calcein for Detecting Flavobacterium columnare and its Assessment in Tilapia Farms.

PubMed

Suebsing, Rungkarn; Kampeera, Jantana; Sirithammajak, Sarawut; Withyachumnarnkul, Boonsirm; Turner, Warren; Kiatpathomchai, Wansika

2015-03-01

Flavobacterium columnare, the causative agent of columnaris disease in fish, affects many economically important freshwater fish species. A colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein (LAMP-calcein) was developed and used to detect the presence of F. columnare in farmed tilapia (Nile Tilapia Oreochromis niloticus and red tilapia [Nile Tilapia × Mozambique Tilapia O. mossambicus]) and rearing water. The detection method, based on a change in color from orange to green, could be performed within 45 min at 63°C. The method was highly specific, as it had no cross-detections with 14 other bacterial species, including other fish pathogens and two Flavobacterium species. The method has a minimum detection limit of 2.2 × 10(2) F. columnare CFU; thus, it is about 10 times more sensitive than conventional PCR. With this method, F. columnare was detected in gonad, gill, and blood samples from apparently healthy tilapia broodstock as well as in samples of fertilized eggs, newly hatched fry, and rearing water. The bacteria isolated from the blood were further characterized biochemically and found to be phenotypically identical to F. columnare. The amplified products from the LAMP-calcein method had 97% homology with the DNA sequence of F. columnare.

Development of a reverse transcription loop-mediated isothermal amplification method for the rapid detection of avian influenza virus subtype H7.

PubMed

Bao, Hongmei; Wang, Xiurong; Zhao, Yuhui; Sun, Xiaodong; Li, Yanbing; Xiong, Yongzhong; Chen, Hualan

2012-01-01

A rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7 avian influenza virus (H7 AIV) isotype was developed. The minimum detection limit of the RT-LAMP assay was 0.1-0.01 PFU per reaction for H7 AIV RNA, making this assay 100-fold more sensitive than the conventional RT-PCR method. This RT-LAMP assay also has the capacity to detect both high- and low-pathogenic H7 AIV strains. Using a pool of RNAs extracted from influenza viruses corresponding to all 15 HA subtypes (in addition to other avian pathogenic viruses), the RT-LAMP system was confirmed to amplify only H7 AIV RNA. Furthermore, specific pathogen free (SPF) chickens were infected artificially with H7 AIV, throat and cloacal swabs were collected, and viral shedding was examined using viral isolation, RT-PCR and RT-LAMP. Shedding was detected following viral isolation and RT-LAMP one day after infection, whereas viral detection using RT-PCR was effective only on day 3 post-infection. These results indicate that the RT-LAMP method could facilitate epidemiological surveillance and the rapid diagnosis of the avian influenza subtype H7. Copyright © 2011 Elsevier B.V. All rights reserved.

Passive blast pressure sensor

DOEpatents

King, Michael J.; Sanchez, Roberto J.; Moss, William C.

2013-03-19

A passive blast pressure sensor for detecting blast overpressures of at least a predetermined minimum threshold pressure. The blast pressure sensor includes a piston-cylinder arrangement with one end of the piston having a detection surface exposed to a blast event monitored medium through one end of the cylinder and the other end of the piston having a striker surface positioned to impact a contact stress sensitive film that is positioned against a strike surface of a rigid body, such as a backing plate. The contact stress sensitive film is of a type which changes color in response to at least a predetermined minimum contact stress which is defined as a product of the predetermined minimum threshold pressure and an amplification factor of the piston. In this manner, a color change in the film arising from impact of the piston accelerated by a blast event provides visual indication that a blast overpressure encountered from the blast event was not less than the predetermined minimum threshold pressure.

Novel Spectrophotometric Method for the Assay of Captopril in Dosage Forms using 2,6-Dichloroquinone-4-Chlorimide

PubMed Central

El-Enany, Nahed; Belal, Fathalla; Rizk, Mohamed

2008-01-01

A simple spectrophotometric method was developed for the determination of captopril (CPL) in pharmaceutical preparations. The method is based on coupling captopril with 2,6-dichloroquinone-4-chlorimide (DCQ) in dimethylsulphoxide. The yellow reaction product was measured at 443 nm. The absorbance–concentration plot was rectilinear over the range of 10-50 μg/mL with minimum detection limit (LOD) of 0.66 μg/mL and a quantification limit (LOQ) of 2.0 μg/mL. The different experimental parameters affecting the development and stability of the color were carefully studied and optimized. The proposed method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained using official and reference spectrophotometric methods. Hydrochlorothiazide which is frequently co-formulated with CPL did not interfere with the assay. A proposal of the reaction pathway was presented. PMID:23675082

Development of a Pneumatic Robot for MRI-guided Transperineal Prostate Biopsy and Brachytherapy: New Approaches

PubMed Central

Song, Sang-Eun; Cho, Nathan B.; Fischer, Gregory; Hata, Nobuhito; Tempany, Clare; Fichtinger, Gabor; Iordachita, Iulian

2011-01-01

Magnetic Resonance Imaging (MRI) guided prostate biopsy and brachytherapy has been introduced in order to enhance the cancer detection and treatment. For the accurate needle positioning, a number of robotic assistants have been developed. However, problems exist due to the strong magnetic field and limited workspace. Pneumatically actuated robots have shown the minimum distraction in the environment but the confined workspace limits optimal robot design and thus controllability is often poor. To overcome the problem, a simple external damping mechanism using timing belts was sought and a 1-DOF mechanism test result indicated sufficient positioning accuracy. Based on the damping mechanism and modular system design approach, a new workspace-optimized 4-DOF parallel robot was developed for the MRI-guided prostate biopsy and brachytherapy. A preliminary evaluation of the robot was conducted using previously developed pneumatic controller and satisfying results were obtained. PMID:21399734

Linear Mode HgCdTe Avalanche Photodiodes for Photon Counting Applications

NASA Technical Reports Server (NTRS)

Sullivan, William, III; Beck, Jeffrey; Scritchfield, Richard; Skokan, Mark; Mitra, Pradip; Sun, Xiaoli; Abshire, James; Carpenter, Darren; Lane, Barry

2015-01-01

An overview of recent improvements in the understanding and maturity of linear mode photon counting with HgCdTe electron-initiated avalanche photodiodes is presented. The first HgCdTe LMPC 2x8 format array fabricated in 2011 with 64 micron pitch was a remarkable success in terms of demonstrating a high single photon signal to noise ratio of 13.7 with an excess noise factor of 1.3-1.4, a 7 ns minimum time between events, and a broad spectral response extending from 0.4 micron to 4.2 micron. The main limitations were a greater than 10x higher false event rate than expected of greater than 1 MHz, a 5-7x lower than expected APD gain, and a photon detection efficiency of only 50% when greater than 60% was expected. This paper discusses the reasons behind these limitations and the implementation of their mitigations with new results.

Analysis of Soft Drinks: UV Spectrophotometry, Liquid Chromatography, and Capillary Electrophoresis

NASA Astrophysics Data System (ADS)

McDevitt, Valerie L.; Rodriguez, Alejandra; Williams, Kathryn R.

1998-05-01

Instrumental analysis students analyze commercial soft drinks in three successive laboratory experiments. First, UV multicomponent analysis is used to determine caffeine and benzoic acid in Mello YelloTM using the spectrophotometer's software and manually by the simultaneous equations method. The following week, caffeine, benzoic acid and aspartame are determined in a variety of soft drinks by reversed-phase liquid chromatography using 45% methanol/55% aqueous phosphate, pH 3.0, as the mobile phase. In the third experiment, the same samples are analyzed by capillary electrophoresis using a pH 9.4 borate buffer. Students also determine the minimum detection limits for all three compounds by both LC and CE. The experiments demonstrate the analytical use and limitations of the three instruments. The reports and prelab quizzes also stress the importance of the chemistry of the three compounds, especially the relationships of acid/base behavior and polarity to the LC and CE separations.

Rapid Inspection of Aerospace Structures - Is It Autonomous Yet?

NASA Technical Reports Server (NTRS)

Bar-Cohen, Yoseph; Backes, Paul; Joffe, Benjamin

1996-01-01

The trend to increase the usage of aging aircraft added a great deal of urgency to the ongoing need for low-cost, rapid, simple-to-operate, reliable and efficient NDE methods for detection and characterization of flaws in aircraft structures. In many cases, the problem of inspection is complex due to the limitation of current technology and the need to disassemble aircraft structures and testing them in lab conditions. To overcome these limitations, reliable field inspection tools are being developed for rapid NDE of large and complex-shape structures, that can operate at harsh, hostal and remote conditions with minimum human interface. In recent years, to address the need for rapid inspection in field conditions, numerous portable scanners were developed using NDE methods, including ultrasonics, shearography, thermography. This paper is written with emphasis on ultrasonic NDE scanners, their evolution and the expected direction of growth.

Improvement in operational characteristics of KEPCO’s line-commutation-type superconducting hybrid fault current limiter

NASA Astrophysics Data System (ADS)

Yim, S.-W.; Park, B.-C.; Jeong, Y.-T.; Kim, Y.-J.; Yang, S.-E.; Kim, W.-S.; Kim, H.-R.; Du, H.-I.

2013-01-01

A 22.9 kV class hybrid fault current limiter (FCL) developed by Korea Electric Power Corporation and LS Industrial Systems in 2006 operates using the line commutation mechanism and begins to limit the fault current after the first half-cycle. The first peak of the fault current is available for protective coordination in the power system. However, it also produces a large electromagnetic force and imposes a huge stress on power facilities such as the main transformer and gas-insulated switchgear. In this study, we improved the operational characteristics of the hybrid FCL in order to reduce the first peak of the fault current. While maintaining the structure of the hybrid FCL system, we developed a superconducting module that detects and limits the fault current during the first half-cycle. To maintain the protective coordination capacity, the hybrid FCL was designed to reduce the first peak value of the fault current by up to approximately 30%. The superconducting module was also designed to produce a minimum AC loss, generating a small, uniform magnetic field distribution during normal operation. Performance tests confirmed that when applied to the hybrid FCL, the superconducting module showed successful current limiting operation without any damage.

Whistle description of Irrawaddy dolphins (Orcaella brevirostris) in Bay of Brunei, Sarawak, Malaysia.

PubMed

Muhamad, Hairul Masrini; Xu, Xiaomei; Zhang, Xuelei; Jaaman, Saifullah Arifin; Muda, Azmi Marzuki

2018-05-01

Studies of Irrawaddy dolphins' acoustics assist in understanding the behaviour of the species and thereby conservation of this species. Whistle signals emitted by Irrawaddy dolphin within the Bay of Brunei in Malaysian waters were characterized. A total of 199 whistles were analysed from seven sightings between January and April 2016. Six types of whistles contours named constant, upsweep, downsweep, concave, convex, and sine were detected when the dolphins engaged in traveling, foraging, and socializing activities. The whistle durations ranged between 0.06 and 3.86 s. The minimum frequency recorded was 443 Hz [Mean = 6000 Hz, standard deviation (SD) = 2320 Hz] and the maximum frequency recorded was 16 071 Hz (Mean = 7139 Hz, SD = 2522 Hz). The mean frequency range (F.R.) for the whistles was 1148 Hz (Minimum F.R. = 0 Hz, Maximum F.R. = 4446 Hz; SD = 876 Hz). Whistles in the Bay of Brunei were compared with population recorded from the waters of Matang and Kalimantan. The comparisons showed differences in whistle duration, minimum frequency, start frequency, and number of inflection point. Variation in whistle occurrence and frequency may be associated with surface behaviour, ambient noise, and recording limitation. This will be an important element when planning a monitoring program.

37 CFR 385.13 - Minimum royalty rates and subscriber-based royalty floors for specific types of services.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., and Copyrights COPYRIGHT ROYALTY BOARD, LIBRARY OF CONGRESS RATES AND TERMS FOR STATUTORY LICENSES... PHYSICAL AND DIGITAL PHONORECORDS Interactive Streaming and Limited Downloads § 385.13 Minimum royalty...

Some Improvements in Utilization of Flash Memory Devices

NASA Technical Reports Server (NTRS)

Gender, Thomas K.; Chow, James; Ott, William E.

2009-01-01

Two developments improve the utilization of flash memory devices in the face of the following limitations: (1) a flash write element (page) differs in size from a flash erase element (block), (2) a block must be erased before its is rewritten, (3) lifetime of a flash memory is typically limited to about 1,000,000 erases, (4) as many as 2 percent of the blocks of a given device may fail before the expected end of its life, and (5) to ensure reliability of reading and writing, power must not be interrupted during minimum specified reading and writing times. The first development comprises interrelated software components that regulate reading, writing, and erasure operations to minimize migration of data and unevenness in wear; perform erasures during idle times; quickly make erased blocks available for writing; detect and report failed blocks; maintain the overall state of a flash memory to satisfy real-time performance requirements; and detect and initialize a new flash memory device. The second development is a combination of hardware and software that senses the failure of a main power supply and draws power from a capacitive storage circuit designed to hold enough energy to sustain operation until reading or writing is completed.

Development and Validation of GC-ECD Method for the Determination of Metamitron in Soil

PubMed Central

Tandon, Shishir; Kumar, Satyendra; Sand, N. K.

2015-01-01

This paper aims at developing and validating a convenient, rapid, and sensitive method for estimation of metamitron from soil samples.Determination andquantification was carried out by Gas Chromatography on microcapillary column with an Electron Capture Detector source. The compound was extracted from soil using methanol and cleanup by C-18 SPE. After optimization, the method was validated by evaluating the analytical curves, linearity, limits of detection, and quantification, precision (repeatability and intermediate precision), and accuracy (recovery). Recovery values ranged from 89 to 93.5% within 0.05- 2.0 µg L−1 with average RSD 1.80%. The precision (repeatability) ranged from 1.7034 to 1.9144% and intermediate precision from 1.5685 to 2.1323%. Retention time was 6.3 minutes, and minimum detectable and quantifiable limits were 0.02 ng mL−1 and 0.05 ng g−1, respectively. Good linearity (R 2 = 0.998) of the calibration curves was obtained over the range from 0.05 to 2.0 µg L−1. Results indicated that the developed method is rapid and easy to perform, making it applicable for analysis in large pesticide monitoring programmes. PMID:25733978

High Surface-Enhanced Raman Scattering (SERS) Amplification Factor Obtained with Silver Printed Circuit Boards and the Influence of Phenolic Resins for the Characterization of the Pesticide Thiram.

PubMed

Silva de Almeida, Francylaine; Bussler, Larissa; Marcio Lima, Sandro; Fiorucci, Antonio Rogério; da Cunha Andrade, Luis Humberto

2016-07-01

In this work, low-cost substrates with rough silver surfaces were prepared from commercial copper foil-covered phenolic board (CPB) and an aqueous solution of AgNO3, and were used for surface-enhanced Raman scattering (SERS) and surface-enhanced resonance Raman scattering (SERRS) measurements. A maximum SERS amplification factor of 1.2 × 10(7) was obtained for Rhodamine 6G (R6G), and use of the CPB resulted in a detection limit for Thiram pesticide of 0.5 µmol L(-1) The minimum detection level was limited by residual traces of phenolic groups that originated from the substrate resin, which became solubilized in the aqueous Ag(+) solution. It was found that the bands corresponding to the impurities had less influence in the Thiram analysis, which could be explained by the high affinity of sulfur for Ag surfaces. The influence of impurities in the SERS analyses therefore depended on the linkage between the rough silver surface and the analyte. The findings demonstrated the ease and effectiveness of using CPB to prepare a nanostructured surface for SERS. © The Author(s) 2016.

The Physics of Extrasolar Gaseous Planets : from Theory to Observable Signatures

NASA Astrophysics Data System (ADS)

Chabrier, G.; Allard, F.; Baraffe, I.; Barman, T.; Hauschildt, P. H.

2004-12-01

We review our present understanding of the physical properties of substellar objects, brown dwarfs and irradiated or non-irradiated gaseous exoplanets. This includes a description of their internal properties, mechanical structure and heat content, their atmospheric properties, thermal profile and emergent spectrum, and their evolution, in particular as irradiated companions of a close parent star. The general theory can be used to make predictions in term of detectability for the future observational projects. Special attention is devoted to the evolution of the two presently detected transit planets, HD 209458b and OGLE-TR-56B. For this latter, we present a consistent evolution for its recently revised mass and show that we reproduce the observed radius within its error bars. We briefly discuss differences between brown dwarfs and gaseous planets, both in terms of mass function and formation process. We outline several arguments to show that the minimum mass for deuterium burning, recently adopted officially as the limit to distinguish the two types of objects, is unlikely to play any specific role in star formation, so that such a limit is of purely semantic nature and is not supported by a physical justification.

The human, primate and rabbit ultraviolet action spectra

NASA Technical Reports Server (NTRS)

Pitts, D. G.; Gibbons, W. D.

1972-01-01

A 5000 watt xenon-mercury high pressure lamp was used to produce a continuous ultraviolet spectrum. Human and animal exposures were made to establish the photokeratitis threshold and abiotic action spectrum. The lower limit of the abiotic action spectrum was 220 nm while the upper limit was 310 nm. The radiant exposure threshold at 270 nm was 0.005 watts/sq cm for the rabbit, 0.004 watts/sq cm for the primate, and 0.004 watts/ sq cm for the human. The rabbit curve was bi-peaked with minimums at 220 nm, 240 nm and 270 nm. The primate curve was tri-peaked with minimums at 220 nm, 240 nm and 270 nm. The human data showed a rather shallow curve with a minimum at 270 nm. Formulas and calculations are given to predict minimum exposure times for ocular damage to man in outer space, to establish valid safety criteria, and to establish protective design criteria.

A new design approach to achieve a minimum impulse limit cycle in the presence of significant measurement uncertainties

NASA Technical Reports Server (NTRS)

Martin, M. W.; Kubiak, E. T.

1982-01-01

A new design was developed for the Space Shuttle Transition Phase Digital Autopilot to reduce the impact of large measurement uncertainties in the rate signal during attitude control. The signal source, which was dictated by early computer constraints, is characterized by large quantization, noise, bias, and transport lag which produce a measurement uncertainty larger than the minimum impulse rate change. To ensure convergence to a minimum impulse limit cycle, the design employed bias and transport lag compensation and a switching logic with hysteresis, rate deadzone, and 'walking' switching line. The design background, the rate measurement uncertainties, and the design solution are documented.

Kuiper belt structure around nearby super-Earth host stars

NASA Astrophysics Data System (ADS)

Kennedy, Grant M.; Matrà, Luca; Marmier, Maxime; Greaves, Jane S.; Wyatt, Mark C.; Bryden, Geoffrey; Holland, Wayne; Lovis, Christophe; Matthews, Brenda C.; Pepe, Francesco; Sibthorpe, Bruce; Udry, Stéphane

2015-05-01

We present new observations of the Kuiper belt analogues around HD 38858 and HD 20794, hosts of super-Earth mass planets within 1 au. As two of the four nearby G-type stars (with HD 69830 and 61 Vir) that form the basis of a possible correlation between low-mass planets and debris disc brightness, these systems are of particular interest. The disc around HD 38858 is well resolved with Herschel and we constrain the disc geometry and radial structure. We also present a probable James Clerk Maxwell Telescope sub-mm continuum detection of the disc and a CO J = 2-1 upper limit. The disc around HD 20794 is much fainter and appears marginally resolved with Herschel, and is constrained to be less extended than the discs around 61 Vir and HD 38858. We also set limits on the radial location of hot dust recently detected around HD 20794 with near-IR interferometry. We present High Accuracy Radial velocity Planet Searcher upper limits on unseen planets in these four systems, ruling out additional super-Earths within a few au, and Saturn-mass planets within 10 au. We consider the disc structure in the three systems with Kuiper belt analogues (HD 69830 has only a warm dust detection), concluding that 61 Vir and HD 38858 have greater radial disc extent than HD 20794. We speculate that the greater width is related to the greater minimum planet masses (10-20 M⊕ versus 3-5 M⊕), arising from an eccentric planetesimal population analogous to the Solar system's scattered disc. We discuss alternative scenarios and possible means to distinguish among them.

Limits to Open Class Performance?

NASA Technical Reports Server (NTRS)

Bowers, Albion H.

2008-01-01

This presentation discusses open or unlimited class aircraft performance limitations and design solutions. Limitations in this class of aircraft include slow climbing flight which requires low wing loading, high cruise speed which requires high wing loading, gains in induced or viscous drag alone which result in only half the gain overall and other structural problems (yaw inertia and spins, flutter and static loads integrity). Design solutions include introducing minimum induced drag for a given span (elliptical span load or winglets) and introducing minimum induced drag for a bell shaped span load. It is concluded that open class performance limits (under current rules and technologies) is very close to absolute limits, though some gains remain to be made from unexplored areas and new technologies.

77 FR 51807 - Agency Forms Undergoing Paperwork Reduction Act Review

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-27

... Minimum Data Elements (MDEs) for the National Breast and Cervical Cancer Early Detection Program (NBCCEDP... screening and early detection tests for breast and cervical cancer. Mammography is extremely valuable as an early detection tool because it can detect breast cancer well before the woman can feel the lump, when...

Synthetic wastewaters treatment by electrocoagulation to remove silver nanoparticles produced by different routes.

PubMed

Matias, M S; Melegari, S P; Vicentini, D S; Matias, W G; Ricordel, C; Hauchard, D

2015-08-15

Nanoscience is a field that has stood out in recent years. The accurate long-term health and environmental risks associated with these emerging materials are unknown. Therefore, this work investigated how to eliminate silver nanoparticles (AgNPs) from synthetic effluents by electrocoagulation (EC) due to the widespread use of this type of nanoparticle (NP) in industry and its potential inhibition power over microorganisms responsible for biological treatment in effluent treatment plants. Synthesized AgNPs were studied via four different routes by chemical reduction in aqueous solutions to simulate the chemical variations of a hypothetical industrial effluent, and efficiency conditions of the EC treatment were determined. All routes used silver nitrate as the source of silver ions, and two synthesis routes were studied with sodium citrate as a stabilizer. In route I, sodium citrate functioned simultaneously as the reducing agent and stabilizing agent, whereas route II used sodium borohydride as a reducing agent. Route III used D-glucose as the reducing agent and sodium pyrophosphate as the stabilizer; route IV used sodium pyrophosphate as the stabilizing agent and sodium borohydride as the reducing agent. The efficiency of the EC process of the different synthesized solutions was studied. For route I, after 85 min of treatment, a significant decrease in the plasmon resonance peak of the sample was observed, which reflects the efficiency in the mass reduction of AgNPs in the solution by 98.6%. In route II, after 12 min of EC, the absorbance results reached the detection limit of the measurement instrument, which indicates a minimum reduction of 99.9% of AgNPs in the solution. During the 4 min of treatment in route III, the absorbance intensities again reached the detection limit, which indicates a minimum reduction of 99.8%. In route IV, after 10 min of treatment, a minimum AgNP reduction of 99.9% was observed. Based on these results, it was possible to verify that the solutions containing citrate considerably increased the necessary times required to eliminate AgNPs from the synthesized effluent, whereas solutions free of this reagent showed better results on floc formation and, therefore, are best for the treatment. The elimination of AgNPs from effluents by EC proved effective for the studied routes. Copyright © 2015 Elsevier Ltd. All rights reserved.

Submicron hard X-ray fluorescence imaging of synthetic elements.

PubMed

Jensen, Mark P; Aryal, Baikuntha P; Gorman-Lewis, Drew; Paunesku, Tatjana; Lai, Barry; Vogt, Stefan; Woloschak, Gayle E

2012-04-13

Synchrotron-based X-ray fluorescence microscopy (XFM) using hard X-rays focused into sub-micron spots is a powerful technique for elemental quantification and mapping, as well as microspectroscopic measurements such as μ-XANES (X-ray absorption near edge structure). We have used XFM to image and simultaneously quantify the transuranic element plutonium at the L(3) or L(2)-edge as well as Th and lighter biologically essential elements in individual rat pheochromocytoma (PC12) cells after exposure to the long-lived plutonium isotope (242)Pu. Elemental maps demonstrate that plutonium localizes principally in the cytoplasm of the cells and avoids the cell nucleus, which is marked by the highest concentrations of phosphorus and zinc, under the conditions of our experiments. The minimum detection limit under typical acquisition conditions with an incident X-ray energy of 18 keV for an average 202 μm(2) cell is 1.4 fg Pu or 2.9×10(-20) moles Pu μm(-2), which is similar to the detection limit of K-edge XFM of transition metals at 10 keV. Copper electron microscopy grids were used to avoid interference from gold X-ray emissions, but traces of strontium present in naturally occurring calcium can still interfere with plutonium detection using its L(α) X-ray emission. Copyright © 2012 Elsevier B.V. All rights reserved.

Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy using a targeted imaging probe

NASA Astrophysics Data System (ADS)

Waterhouse, Dale J.; Joseph, James; Neves, Andre A.; di Pietro, Massimiliano; Brindle, Kevin M.; Fitzgerald, Rebecca C.; Bohndiek, Sarah E.

2016-03-01

Barrett's esophagus is a condition that predisposes patients to esophageal cancer. Early detection of cancer in these patients can be curative, but is confounded by a lack of contrast in white light endoscopy (WLE). Application of fluorescently-labeled lectins to the esophagus during endoscopy can more accurately delineate dysplasia emerging within Barrett's than WLE1, but strong tissue autofluorescence has limited sensitivity and dynamic range of this approach. To overcome this challenge, we synthesized a near-infrared (NIR) fluorescent lectin and have constructed a clinically translatable endoscope for simultaneous WLE and NIR imaging. An imaging fiber bundle, shielded from patient contact using a disposable catheter, relays collected light into an optical path that splits the WL reflectance and NIR emission onto two cameras for simultaneous video-rate recording. The captured images are co-registered and the honeycomb artifact arising from the fiber bundle is removed using interpolation between image points derived from individual fibers. A minimum detectable concentration of 110 nM was determined using a dilution series of IRDye800CW-lectin in black well plates. We have demonstrated the ability to use our endoscope to distinguish between different tissue types in ex vivo mouse stomachs. Future work using human ex vivo tissue specimens will determine safe illumination limits and sensitivity for dysplasia and adenocarcinoma in Barrett's esophagus, prior to commencing clinical trials.

The investigation of solar activity signals by analyzing of tree ring chronological scales

NASA Astrophysics Data System (ADS)

Nickiforov, M. G.

2017-07-01

The present study examines the ability of detecting short-cycles and global minima of solar activity by analyzing dendrochronologies. Starting with the study of Douglass, which was devoted to the question of climatic cycles and the growth of trees, it is believed that the analysis of dendrochronologies allows to detect the cycle of Wolf-Schwabe. According to his results, the cycle was absent during Maunder's minimum and appeared after its completion. Having checked Douglass's conclusions by using 10 dendrochronologies of yellow pines from Arizona, which cover the time period from 1600 to 1900, we have come to the opposite results. The verification shows that: a) none of the considered dendroscale allows to detect an 11-year cycle; 2) the behaviour of a short peroid-signal does not undergo significant changes before, during or after Maunder's minimum. A similar attempt to detect global minima of solar activity by using five dendrochronologies from different areas has not led to positive results. On the one hand, the signal of global extremum is not always recorded in dendrochronology, on the other hand, the deep depression of annual rings allows to suppose the existence of a global minimum of solar activity, which is actually absent.

Real-time diagnostics of a jet engine exhaust using an intra-pulse quantum cascade laser spectrometer

NASA Astrophysics Data System (ADS)

Duxbury, Geoffrey; Hay, Kenneth G.; Langford, Nigel; Johnson, Mark P.; Black, John D.

2011-09-01

It has been demonstrated that an intra-pulse scanned quantum cascade laser spectrometer may be used to obtain real-time diagnostics of the amounts of carbon monoxide, carbon dioxide, and water, in the exhaust of an aero gas turbine (turbojet) engine operated in a sea level test cell. Measurements have been made of the rapid changes in composition following ignition, the composition under steady state operating conditions, and the composition changes across the exhaust plume. The minimum detection limit for CO in a double pass through a typical gas turbine plume of 50 cm in diameter, with 0.4 seconds integration time, is approximately 2 ppm.

An 'X-banded' Tidbinbilla interferometer

NASA Technical Reports Server (NTRS)

Batty, Michael J.; Gardyne, R. G.; Gay, G. J.; Jauncy, David L.; Gulkis, S.; Kirk, A.

1986-01-01

The recent upgrading of the Tidbinbilla two-element interferometer to simultaneous S-band (2.3 GHz) and X-band (8.4 GHz) operation has provided a powerful new astronomical facility for weak radio source measurement in the Southern Hemisphere. The new X-band system has a minimum fringe spacing of 38 arcsec, and about the same positional measurement capability (approximately 2 arcsec) and sensitivity (1 s rms noise of 10 mJy) as the previous S-band system. However, the far lower confusion limit will allow detection and accurate positional measurements for sources as weak as a few millijanskys. This capability will be invaluable for observations of radio stars, X-ray sources and other weak, compact radio sources.

50 CFR 218.174 - Requirements for monitoring and reporting.

Code of Federal Regulations, 2011 CFR

2011-10-01

...-based surveys shall be designed to maximize detections of marine mammals near mission activity event. (2... Navy to implement, at a minimum, the monitoring activities summarized below: (1) Visual Surveys: (i) The Holder of this Authorization shall conduct a minimum of 2 special visual surveys per year to...

Performance of a neutron spectrometer based on a PIN diode.

PubMed

Agosteo, S; D'Angelo, G; Fazzi, A; Para, A Foglio; Pola, A; Ventura, L; Zotto, P

2005-01-01

The neutron spectrometer discussed in this work consists of a PIN diode coupled with a polyethylene converter. Neutrons are detected through the energy deposited by recoil-protons in silicon. The maximum detectable energy is -6 MeV and is imposed by the thickness of the fully depleted layer (300 microm for the present device). The minimum detectable energy which can be assessed with pulse-shape discrimination (PSD) is -0.9 MeV. PSD is performed with a crossover method and setting the diode in the 'reverse-injection' configuration (i.e. with the N+ layer adjacent to the converter). This configuration provides longer collection times for the electron-hole pairs generated by the recoil-protons. The limited interval of detectable energies restricts the application of this spectrometer to low-energy neutron fields, such as the ones which can be produced at facilities hosting low-energy ion accelerators. The capacity to reproduce continuous neutron spectra was investigated by optimising the electronic chain for pulse-shape discrimination. In particular, the spectrometer was irradiated with neutrons that were generated by striking a thick beryllium target with protons of several energies and the measured spectra were compared with data taken from the literature.

Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus

PubMed Central

Bao, Hongmei; Zhao, Yuhui; Wang, Yunhe; Xu, Xiaolong; Shi, Jianzhong; Zeng, Xianying; Wang, Xiurong; Chen, Hualan

2014-01-01

A novel influenza A (H7N9) virus has emerged in China. To rapidly detect this virus from clinical samples, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7N9 virus. The minimum detection limit of the RT-LAMP assay was 0.01 PFU H7N9 virus, making this method 100-fold more sensitive to the detection of the H7N9 virus than conventional RT-PCR. The H7N9 virus RT-LAMP assays can efficiently detect different sources of H7N9 influenza virus RNA (from chickens, pigeons, the environment, and humans). No cross-reactive amplification with the RNA of other subtype influenza viruses or of other avian respiratory viruses was observed. The assays can effectively detect H7N9 influenza virus RNA in drinking water, soil, cloacal swab, and tracheal swab samples that were collected from live poultry markets, as well as human H7N9 virus, in less than 30 min. These results suggest that the H7N9 virus RT-LAMP assays were efficient, practical, and rapid diagnostic methods for the epidemiological surveillance and diagnosis of influenza A (H7N9) virus from different resource samples. PMID:24689044

Toward in vivo detection of hydrogen peroxide with ultrasound molecular imaging

PubMed Central

Olson, Emilia S.; Orozco, Jahir; Wu, Zhe; Malone, Christopher D.; Yi, Boemha; Gao, Wei; Eghtedari, Mohammad; Wang, Joseph; Mattrey, Robert F.

2013-01-01

We present a new class of ultrasound molecular imaging agents that extend upon the design of micromotors that are designed to move through fluids by catalyzing hydrogen peroxide (H2O2) and propelling forward by escaping oxygen microbubbles. Micromotor converters require 62 mm of H2O2 to move – 1000-fold higher than is expected in vivo. Here, we aim to prove that ultrasound can detect the expelled microbubbles, to determine the minimum H2O2 concentration needed for microbubble detection, explore alternate designs to detect the H2O2 produced by activated neutrophils and perform preliminary in vivo testing. Oxygen microbubbles were detected by ultrasound at 2.5 mm H2O2. Best results were achieved with a 400–500 nm spherical design with alternating surface coatings of catalase and PSS over a silica core. The lowest detection limit of 10–100 µm was achieved when assays were done in plasma. Using this design, we detected the H2O2 produced by freshly isolated PMA-activated neutrophils allowing their distinction from naïve neutrophils. Finally, we were also able to show that direct injection of these nanospheres into an abscess in vivo enhanced ultrasound signal only when they contained catalase, and only when injected into an abscess, likely because of the elevated levels of H2O2 produced by inflammatory mediators. PMID:23958028

Demonstration of a portable near-infrared CH4 detection sensor based on tunable diode laser absorption spectroscopy

NASA Astrophysics Data System (ADS)

Zheng, Chuan-Tao; Huang, Jian-Qiang; Ye, Wei-Lin; Lv, Mo; Dang, Jing-Min; Cao, Tian-Shu; Chen, Chen; Wang, Yi-Ding

2013-11-01

A portable near-infrared (NIR) CH4 detection sensor based on a distributed feedback (DFB) laser modulated at 1.654 μm is experimentally demonstrated. Intelligent temperature controller with an accuracy of -0.07 to +0.09 °C as well as a scan and modulation module generating saw-wave and cosine-wave signals are developed to drive the DFB laser, and a cost effective lock-in amplifier used to extract the second harmonic signal is integrated. Thorough experiments are carried out to obtain detection performances, including detection range, accuracy, stability and the minimum detection limit (MDL). Measurement results show that the absolute detection error relative to the standard value is less than 7% within the range of 0-100%, and the MDL is estimated to be about 11 ppm under an absorption length of 0.2 m and a noise level of 2 mVpp. Twenty-four hours monitoring on two gas samples (0.1% and 20%) indicates that the absolute errors are less than 7% and 2.5%, respectively, suggesting good long term stability. The sensor reveals competitive characteristics compared with other reported portable or handheld sensors. The developed sensor can also be used for the detection of other gases by adopting other DFB lasers with different center-wavelength using the same hardware and slightly modified software.

Portable GMR Handheld Platform for the Detection of Influenza A Virus.

PubMed

Wu, Kai; Klein, Todd; Krishna, Venkatramana D; Su, Diqing; Perez, Andres M; Wang, Jian-Ping

2017-11-22

Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID 50 /mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.

Laser ablation molecular isotopic spectroscopy (LAMIS) towards the determination of multivariate LODs via PLS calibration model of 10B and 11B Boric acid mixtures

NASA Astrophysics Data System (ADS)

Harris, C. D.; Profeta, Luisa T. M.; Akpovo, Codjo A.; Johnson, Lewis; Stowe, Ashley C.

2017-05-01

A calibration model was created to illustrate the detection capabilities of laser ablation molecular isotopic spectroscopy (LAMIS) discrimination in isotopic analysis. The sample set contained boric acid pellets that varied in isotopic concentrations of 10B and 11B. Each sample set was interrogated with a Q-switched Nd:YAG ablation laser operating at 532 nm. A minimum of four band heads of the β system B2∑ -> Χ2∑transitions were identified and verified with previous literature on BO molecular emission lines. Isotopic shifts were observed in the spectra for each transition and used as the predictors in the calibration model. The spectra along with their respective 10/11B isotopic ratios were analyzed using Partial Least Squares Regression (PLSR). An IUPAC novel approach for determining a multivariate Limit of Detection (LOD) interval was used to predict the detection of the desired isotopic ratios. The predicted multivariate LOD is dependent on the variation of the instrumental signal and other composites in the calibration model space.

Development of microcontroller-based acquisition and processing unit for fiber optic vibration sensor

NASA Astrophysics Data System (ADS)

Suryadi; Puranto, P.; Adinanta, H.; Waluyo, T. B.; Priambodo, P. S.

2017-04-01

Microcontroller based acquisition and processing unit (MAPU) has been developed to measure vibration signal from fiber optic vibration sensor. The MAPU utilizes a 32-bit ARM microcontroller to perform acquisition and processing of the input signal. The input signal is acquired with 12 bit ADC and processed using FFT method to extract frequency information. Stability of MAPU is characterized by supplying a constant input signal at 500 Hz for 29 hours and shows a stable operation. To characterize the frequency response, input signal is swapped from 20 to 1000 Hz with 20 Hz interval. The characterization result shows that MAPU can detect input signal from 20 to 1000 Hz with minimum signal of 4 mV RMS. The experiment has been set that utilizes the MAPU with singlemode-multimode-singlemode (SMS) fiber optic sensor to detect vibration which is induced by a transducer in a wooden platform. The experimental result indicates that vibration signal from 20 to 600 Hz has been successfully detected. Due to the limitation of the vibration source used in the experiment, vibration signal above 600 Hz is undetected.

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequencemore » (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.« less

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

DOE PAGES

Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas; ...

2017-08-08

Here, we present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a MetagenomeAssembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Genemore » Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.« less

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas

Here, we present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a MetagenomeAssembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Genemore » Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.« less

50 CFR 622.275 - Size limits.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.275 Size limits. All size limits in this section are minimum size...

50 CFR 622.275 - Size limits.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.275 Size limits. All size limits in this section are minimum size...

Fully invariant wavelet enhanced minimum average correlation energy filter for object recognition in cluttered and occluded environments

NASA Astrophysics Data System (ADS)

Tehsin, Sara; Rehman, Saad; Riaz, Farhan; Saeed, Omer; Hassan, Ali; Khan, Muazzam; Alam, Muhammad S.

2017-05-01

A fully invariant system helps in resolving difficulties in object detection when camera or object orientation and position are unknown. In this paper, the proposed correlation filter based mechanism provides the capability to suppress noise, clutter and occlusion. Minimum Average Correlation Energy (MACE) filter yields sharp correlation peaks while considering the controlled correlation peak value. Difference of Gaussian (DOG) Wavelet has been added at the preprocessing stage in proposed filter design that facilitates target detection in orientation variant cluttered environment. Logarithmic transformation is combined with a DOG composite minimum average correlation energy filter (WMACE), capable of producing sharp correlation peaks despite any kind of geometric distortion of target object. The proposed filter has shown improved performance over some of the other variant correlation filters which are discussed in the result section.

Implications of Liebig’s law of the minimum for tree-ring reconstructions of climate

NASA Astrophysics Data System (ADS)

Stine, A. R.; Huybers, P.

2017-11-01

A basic principle of ecology, known as Liebig’s Law of the Minimum, is that plant growth reflects the strongest limiting environmental factor. This principle implies that a limiting environmental factor can be inferred from historical growth and, in dendrochronology, such reconstruction is generally achieved by averaging collections of standardized tree-ring records. Averaging is optimal if growth reflects a single limiting factor and noise but not if growth also reflects locally variable stresses that intermittently limit growth. In this study a collection of Arctic tree ring records is shown to follow scaling relationships that are inconsistent with the signal-plus-noise model of tree growth but consistent with Liebig’s Law acting at the local level. Also consistent with law-of-the-minimum behavior is that reconstructions based on the least-stressed trees in a given year better-follow variations in temperature than typical approaches where all tree-ring records are averaged. Improvements in reconstruction skill occur across all frequencies, with the greatest increase at the lowest frequencies. More comprehensive statistical-ecological models of tree growth may offer further improvement in reconstruction skill.

Relation between Streamflow of Swiftcurrent Creek, Montana, and the Geometry of Passage for Bull Trout (Salvelinus confluentus)

USGS Publications Warehouse

Auble, Gregor T.; Holmquist-Johnson, Christopher L.; Mogen, Jim T.; Kaeding, Lynn R.; Bowen, Zachary H.

2009-01-01

Operation of Sherburne Dam in northcentral Montana has typically reduced winter streamflow in Swiftcurrent Creek downstream of the dam and resulted in passage limitations for bull trout (Salvelinus confluentus). We defined an empirical relation between discharge in Swiftcurrent Creek between Sherburne Dam and the downstream confluence with Boulder Creek and fish passage geometry by considering how the cross-sectional area of water changed as a function of discharge at a set of cross sections likely to limit fish passage. With a minimum passage window of 15 x 45 cm, passage at the cross sections increased strongly with discharge over the range of 1.2 to 24 cfs. Most cross sections did not satisfy the minimum criteria at 1.2 cfs, 25 percent had no passage at 12.7 cfs, whereas at 24 cfs all but one of 26 cross sections had some passage and 90 percent had more than 3 m of width satisfying the minimum criteria. Sensitivity analysis suggests that the overall results are not highly dependent on exact dimensions of the minimum passage window. Combining these results with estimates of natural streamflow in the study reach further suggests that natural streamflow provided adequate passage at some times in most months and locations in the study reach, although not for all individual days and locations. Limitations of our analysis include assumptions about minimum passage geometry, measurement error, limitations of the cross-sectional model we used to characterize passage, the relation of Sherburne Dam releases to streamflow in the downstream study reach in the presence of ephemeral accretions, and the relation of passage geometry as we have measured it to fish responses of movement, stranding, and mortality, especially in the presence of ice cover.

Automatic Ship Detection in Remote Sensing Images from Google Earth of Complex Scenes Based on Multiscale Rotation Dense Feature Pyramid Networks

NASA Astrophysics Data System (ADS)

Yang, Xue; Sun, Hao; Fu, Kun; Yang, Jirui; Sun, Xian; Yan, Menglong; Guo, Zhi

2018-01-01

Ship detection has been playing a significant role in the field of remote sensing for a long time but it is still full of challenges. The main limitations of traditional ship detection methods usually lie in the complexity of application scenarios, the difficulty of intensive object detection and the redundancy of detection region. In order to solve such problems above, we propose a framework called Rotation Dense Feature Pyramid Networks (R-DFPN) which can effectively detect ship in different scenes including ocean and port. Specifically, we put forward the Dense Feature Pyramid Network (DFPN), which is aimed at solving the problem resulted from the narrow width of the ship. Compared with previous multi-scale detectors such as Feature Pyramid Network (FPN), DFPN builds the high-level semantic feature-maps for all scales by means of dense connections, through which enhances the feature propagation and encourages the feature reuse. Additionally, in the case of ship rotation and dense arrangement, we design a rotation anchor strategy to predict the minimum circumscribed rectangle of the object so as to reduce the redundant detection region and improve the recall. Furthermore, we also propose multi-scale ROI Align for the purpose of maintaining the completeness of semantic and spatial information. Experiments based on remote sensing images from Google Earth for ship detection show that our detection method based on R-DFPN representation has a state-of-the-art performance.

Highly sensitive and selective lateral flow immunoassay based on magnetic nanoparticles for quantitative detection of carcinoembryonic antigen.

PubMed

Liu, Fangming; Zhang, Honglian; Wu, Zhenhua; Dong, Haidao; Zhou, Lin; Yang, Dawei; Ge, Yuqing; Jia, Chunping; Liu, Huiying; Jin, Qinghui; Zhao, Jianlong; Zhang, Qiqing; Mao, Hongju

2016-12-01

Carcinoembryonic antigen (CEA) is an important biomarker in cancer diagnosis. Here, we present an efficient, selective lateral-flow immunoassay (LFIA) based on magnetic nanoparticles (MNPs) for in situ sensitive and accurate point-of-care detection of CEA. Signal amplification mechanism involved linking of detection MNPs with signal MNPs through biotin-modified single-stranded DNA (ssDNA) and streptavidin. To verify the effectiveness of this modified LFIA system, the sensitivity and specificity were evaluated. Sensitivity evaluation showed a broad detection range of 0.25-1000ng/ml for CEA protein by the modified LFIA, and the limit of detection (LOD) of the modified LFIA was 0.25ng/ml, thus producing significant increase in detection threshold compared with the traditional LFIA. The modified LFIA could selectively recognize CEA in presence of several interfering proteins. In addition, this newly developed assay was applied for quantitative detection of CEA in human serum specimens collected from 10 randomly selected patients. The modified LFIA system detected minimum 0.27ng/ml of CEA concentration in serum samples. The results were consistent with the clinical data obtained using commercial electrochemiluminescence immunoassay (ECLIA) (p<0.01). In conclusion, the MNPs based LFIA system not only demonstrated enhanced signal to noise ratio, it also detected CEA with higher sensitivity and selectivity, and thus has great potential to be commercially applied as a sensitive tumor marker filtration system. Copyright © 2016 Elsevier B.V. All rights reserved.

Assessing Seasonal and Inter-Annual Variations of Lake Surface Areas in Mongolia during 2000-2011 Using Minimum Composite MODIS NDVI

PubMed Central

Kang, Sinkyu; Hong, Suk Young

2016-01-01

A minimum composite method was applied to produce a 15-day interval normalized difference vegetation index (NDVI) dataset from Moderate Resolution Imaging Spectroradiometer (MODIS) daily 250 m reflectance in the red and near-infrared bands. This dataset was applied to determine lake surface areas in Mongolia. A total of 73 lakes greater than 6.25 km2in area were selected, and 28 of these lakes were used to evaluate detection errors. The minimum composite NDVI showed a better detection performance on lake water pixels than did the official MODIS 16-day 250 m NDVI based on a maximum composite method. The overall lake area detection performance based on the 15-day minimum composite NDVI showed -2.5% error relative to the Landsat-derived lake area for the 28 evaluated lakes. The errors increased with increases in the perimeter-to-area ratio but decreased with lake size over 10 km2. The lake area decreased by -9.3% at an annual rate of -53.7 km2 yr-1 during 2000 to 2011 for the 73 lakes. However, considerable spatial variations, such as slight-to-moderate lake area reductions in semi-arid regions and rapid lake area reductions in arid regions, were also detected. This study demonstrated applicability of MODIS 250 m reflectance data for biweekly monitoring of lake area change and diagnosed considerable lake area reduction and its spatial variability in arid and semi-arid regions of Mongolia. Future studies are required for explaining reasons of lake area changes and their spatial variability. PMID:27007233

Assessing Seasonal and Inter-Annual Variations of Lake Surface Areas in Mongolia during 2000-2011 Using Minimum Composite MODIS NDVI.

PubMed

Kang, Sinkyu; Hong, Suk Young

2016-01-01

A minimum composite method was applied to produce a 15-day interval normalized difference vegetation index (NDVI) dataset from Moderate Resolution Imaging Spectroradiometer (MODIS) daily 250 m reflectance in the red and near-infrared bands. This dataset was applied to determine lake surface areas in Mongolia. A total of 73 lakes greater than 6.25 km2in area were selected, and 28 of these lakes were used to evaluate detection errors. The minimum composite NDVI showed a better detection performance on lake water pixels than did the official MODIS 16-day 250 m NDVI based on a maximum composite method. The overall lake area detection performance based on the 15-day minimum composite NDVI showed -2.5% error relative to the Landsat-derived lake area for the 28 evaluated lakes. The errors increased with increases in the perimeter-to-area ratio but decreased with lake size over 10 km(2). The lake area decreased by -9.3% at an annual rate of -53.7 km(2) yr(-1) during 2000 to 2011 for the 73 lakes. However, considerable spatial variations, such as slight-to-moderate lake area reductions in semi-arid regions and rapid lake area reductions in arid regions, were also detected. This study demonstrated applicability of MODIS 250 m reflectance data for biweekly monitoring of lake area change and diagnosed considerable lake area reduction and its spatial variability in arid and semi-arid regions of Mongolia. Future studies are required for explaining reasons of lake area changes and their spatial variability.

Differential detection of Gaussian MSK in a mobile radio environment

NASA Technical Reports Server (NTRS)

Simon, M. K.; Wang, C. C.

1984-01-01

Minimum shift keying with Gaussian shaped transmit pulses is a strong candidate for a modulation technique that satisfies the stringent out-of-band radiated power requirements of the mobil radio application. Numerous studies and field experiments have been conducted by the Japanese on urban and suburban mobile radio channels with systems employing Gaussian minimum-shift keying (GMSK) transmission and differentially coherent reception. A comprehensive analytical treatment is presented of the performance of such systems emphasizing the important trade-offs among the various system design parameters such as transmit and receiver filter bandwidths and detection threshold level. It is shown that two-bit differential detection of GMSK is capable of offering far superior performance to the more conventional one-bit detection method both in the presence of an additive Gaussian noise background and Rician fading.

Differential detection of Gaussian MSK in a mobile radio environment

NASA Astrophysics Data System (ADS)

Simon, M. K.; Wang, C. C.

1984-11-01

Minimum shift keying with Gaussian shaped transmit pulses is a strong candidate for a modulation technique that satisfies the stringent out-of-band radiated power requirements of the mobil radio application. Numerous studies and field experiments have been conducted by the Japanese on urban and suburban mobile radio channels with systems employing Gaussian minimum-shift keying (GMSK) transmission and differentially coherent reception. A comprehensive analytical treatment is presented of the performance of such systems emphasizing the important trade-offs among the various system design parameters such as transmit and receiver filter bandwidths and detection threshold level. It is shown that two-bit differential detection of GMSK is capable of offering far superior performance to the more conventional one-bit detection method both in the presence of an additive Gaussian noise background and Rician fading.

Net analyte signal-based simultaneous determination of ethanol and water by quartz crystal nanobalance sensor.

PubMed

Mirmohseni, A; Abdollahi, H; Rostamizadeh, K

2007-02-28

Net analyte signal (NAS)-based method called HLA/GO was applied for the selectively determination of binary mixture of ethanol and water by quartz crystal nanobalance (QCN) sensor. A full factorial design was applied for the formation of calibration and prediction sets in the concentration ranges 5.5-22.2 microg mL(-1) for ethanol and 7.01-28.07 microg mL(-1) for water. An optimal time range was selected by procedure which was based on the calculation of the net analyte signal regression plot in any considered time window for each test sample. A moving window strategy was used for searching the region with maximum linearity of NAS regression plot (minimum error indicator) and minimum of PRESS value. On the base of obtained results, the differences on the adsorption profiles in the time range between 1 and 600 s were used to determine mixtures of both compounds by HLA/GO method. The calculation of the net analytical signal using HLA/GO method allows determination of several figures of merit like selectivity, sensitivity, analytical sensitivity and limit of detection, for each component. To check the ability of the proposed method in the selection of linear regions of adsorption profile, a test for detecting non-linear regions of adsorption profile data in the presence of methanol was also described. The results showed that the method was successfully applied for the determination of ethanol and water.

Reliability and convergent validity of the five-step test in people with chronic stroke.

PubMed

Ng, Shamay S M; Tse, Mimi M Y; Tam, Eric W C; Lai, Cynthia Y Y

2018-01-10

(i) To estimate the intra-rater, inter-rater and test-retest reliabilities of the Five-Step Test (FST), as well as the minimum detectable change in FST completion times in people with stroke. (ii) To estimate the convergent validity of the FST with other measures of stroke-specific impairments. (iii) To identify the best cut-off times for distinguishing FST performance in people with stroke from that of healthy older adults. A cross-sectional study. University-based rehabilitation centre. Forty-eight people with stroke and 39 healthy controls. None. The FST, along with (for the stroke survivors only) scores on the Fugl-Meyer Lower Extremity Assessment (FMA-LE), the Berg Balance Scale (BBS), Limits of Stability (LOS) tests, and Activities-specific Balance Confidence (ABC) scale were tested. The FST showed excellent intra-rater (intra-class correlation coefficient; ICC = 0.866-0.905), inter-rater (ICC = 0.998), and test-retest (ICC = 0.838-0.842) reliabilities. A minimum detectable change of 9.16 s was found for the FST in people with stroke. The FST correlated significantly with the FMA-LE, BBS, and LOS results in the forward and sideways directions (r = -0.411 to -0.716, p < 0.004). The FST completion time of 13.35 s was shown to discriminate reliably between people with stroke and healthy older adults. The FST is a reliable, easy-to-administer clinical test for assessing stroke survivors' ability to negotiate steps and stairs.

USING SUSTAINABILITY CRITERIA TO GUIDE LAND-USE DECISIONS

EPA Science Inventory

Human society and our economic systems are limited by the capacities of natural systems, and the sustainability of natural systems will be dependent upon the attainment of undetermined but minimum conditions of social and economic systems. These minimum conditions of intact econo...

41 CFR 101-25.301 - General.

Code of Federal Regulations, 2010 CFR

2010-07-01

... which shall be applied by all executive agencies. Additional criteria above these minimum standards shall be established by each executive agency, limiting its property to the minimum requirements necessary for the efficient functioning of the particular office concerned. This subpart does not apply to...

Survey of Occupational Noise Exposure in CF Personnel in Selected High-Risk Trades

DTIC Science & Technology

2003-11-01

peak, maximum level , minimum level , average sound level , time weighted average, dose, projected 8-hour dose, and upper limit time were measured for...10 4.4.2 Maximum Sound Level ...11 4.4.3 Minimum Sound Level

High-volume extraction of nucleic acids by magnetic bead technology for ultrasensitive detection of bacteria in blood components.

PubMed

Störmer, Melanie; Kleesiek, Knut; Dreier, Jens

2007-01-01

Nucleic acid isolation, the most technically demanding and laborious procedure performed in molecular diagnostics, harbors the potential for improvements in automation. A recent development is the use of magnetic beads covered with nucleic acid-binding matrices. We adapted this technology with a broad-range 23S rRNA real-time reverse transcription (RT)-PCR assay for fast and sensitive detection of bacterial contamination of blood products. We investigated different protocols for an automated high-volume extraction method based on magnetic-separation technology for the extraction of bacterial nucleic acids from platelet concentrates (PCs). We added 2 model bacteria, Staphylococcus epidermidis and Escherichia coli, to a single pool of apheresis-derived, single-donor platelets and assayed the PCs by real-time RT-PCR analysis with an improved primer-probe system and locked nucleic acid technology. Co-amplification of human beta(2)-microglobulin mRNA served as an internal control (IC). We used probit analysis to calculate the minimum concentration of bacteria that would be detected with 95% confidence. For automated magnetic bead-based extraction technology with the real-time RT-PCR, the 95% detection limit was 29 x 10(3) colony-forming units (CFU)/L for S. epidermidis and 22 x 10(3) CFU/L for E. coli. No false-positive results occurred, either due to nucleic acid contamination of reagents or externally during testing of 1030 PCs. High-volume nucleic acid extraction improved the detection limit of the assay. The improvement of the primer-probe system and the integration of an IC make the RT-PCR assay appropriate for bacteria screening of platelets.

Colonial waterbird predation on Lost River and Shortnose suckers in the Upper Klamath Basin

USGS Publications Warehouse

Evans, Allen F.; Hewitt, David A.; Payton, Quinn; Cramer, Bradley M.; Collis, Ken; Roby, Daniel D.

2016-01-01

We evaluated predation on Lost River Suckers Deltistes luxatus and Shortnose Suckers Chasmistes brevirostris by American white pelicans Pelecanus erythrorhynchos and double-crested cormorants Phalacrocorax auritus nesting at mixed-species colonies in the Upper Klamath Basin of Oregon and California during 2009–2014. Predation was evaluated by recovering (detecting) PIT tags from tagged fish on bird colonies and calculating minimum predation rates, as the percentage of available suckers consumed, adjusted for PIT tag detection probabilities but not deposition probabilities (i.e., probability an egested tag was deposited on- or off-colony). Results indicate that impacts of avian predation varied by sucker species, age-class (adult, juvenile), bird colony location, and year, demonstrating dynamic predator–prey interactions. Tagged suckers ranging in size from 72 to 730 mm were susceptible to cormorant or pelican predation; all but the largest Lost River Suckers were susceptible to bird predation. Minimum predation rate estimates ranged annually from <0.1% to 4.6% of the available PIT-tagged Lost River Suckers and from <0.1% to 4.2% of the available Shortnose Suckers, and predation rates were consistently higher on suckers in Clear Lake Reservoir, California, than on suckers in Upper Klamath Lake, Oregon. There was evidence that bird predation on juvenile suckers (species unknown) in Upper Klamath Lake was higher than on adult suckers in Upper Klamath Lake, where minimum predation rates ranged annually from 5.7% to 8.4% of available juveniles. Results suggest that avian predation is a factor limiting the recovery of populations of Lost River and Shortnose suckers, particularly juvenile suckers in Upper Klamath Lake and adult suckers in Clear Lake Reservoir. Additional research is needed to measure predator-specific PIT tag deposition probabilities (which, based on other published studies, could increase predation rates presented herein by a factor of roughly 2.0) and to better understand biotic and abiotic factors that regulate sucker susceptibility to bird predation.

Some Observations on Damage Tolerance Analyses in Pressure Vessels

NASA Technical Reports Server (NTRS)

Raju, Ivatury S.; Dawicke, David S.; Hampton, Roy W.

2017-01-01

AIAA standards S080 and S081 are applicable for certification of metallic pressure vessels (PV) and composite overwrap pressure vessels (COPV), respectively. These standards require damage tolerance analyses with a minimum reliable detectible flaw/crack and demonstration of safe life four times the service life with these cracks at the worst-case location in the PVs and oriented perpendicular to the maximum principal tensile stress. The standards require consideration of semi-elliptical surface cracks in the range of aspect ratios (crack depth a to half of the surface length c, i.e., (a/c) of 0.2 to 1). NASA-STD-5009 provides the minimum reliably detectible standard crack sizes (90/95 probability of detection (POD) for several non-destructive evaluation (NDE) methods (eddy current (ET), penetrant (PT), radiography (RT) and ultrasonic (UT)) for the two limits of the aspect ratio range required by the AIAA standards. This paper tries to answer the questions: can the safe life analysis consider only the life for the crack sizes at the two required limits, or endpoints, of the (a/c) range for the NDE method used or does the analysis need to consider values within that range? What would be an appropriate method to interpolate 90/95 POD crack sizes at intermediate (a/c) values? Several procedures to develop combinations of a and c within the specified range are explored. A simple linear relationship between a and c is chosen to compare the effects of seven different approaches to determine combinations of aj and cj that are between the (a/c) endpoints. Two of the seven are selected for evaluation: Approach I, the simple linear relationship, and a more conservative option, Approach III. For each of these two Approaches, the lives are computed for initial semi-elliptic crack configurations in a plate subjected to remote tensile fatigue loading with an R-ratio of 0.1, for an assumed material evaluated using NASGRO (registered 4) version 8.1. These calculations demonstrate that for this loading, using Approach I and the initial detectable crack sizes at the (a/c) endpoints in 5009 specified for the ET and UT NDE methods, the smallest life is not at the two required limits of the (a/c) range, but rather is at an intermediate configuration in the range (a/c) of 0.4 to 0.6. Similar analyses using both Approach I and III with the initial detectable crack size at the (a/c) endpoints in 5009 for PT NDE showed the smallest life may be at an (a/c) endpoint or an intermediate (a/c), depending upon which Approach is used. As such, analyses that interrogate only the two (a/c) values of 0.2 and 1 may result in unconservative life predictions. The standard practice may need to be revised based on these results.

BACKGROUND TRACK DENSITY REDUCTION OF 50-HZ-HV ECE-PROCESSED THICK POLYCARBONATE DETECTORS TO IMPROVE LOWER DETECTION LIMIT.

PubMed

Sohrabi, M; Hakimi, A; Soltani, Z

2016-12-01

A recent novel development of 50-Hz-HV ECE of 1-mm-thick and 250-µm-thick polycarbonate track detectors (PCTDs) has proved some promising results for some health physics, dosimetry and ion-beam-related applications. The method while proved having some good characteristics for some applications provided a relatively higher background track density (BGTD) in particular when very high voltages are applied to the PCTDs. In order to decrease the minimum detection limit (MDL) of the PCTDs and to further promote its applications for low dose, the BGTD was reduced by applying a layer removal methodology applying ethylendiamine (EDA). The effects of EDA concentrations (50, 60, 65, 70, 75, 80, 85 and 90 %) in water at room temperature (26°C) and soaking durations up to 100 min at different EDA concentration on BGTD reduction were studied. The thickness of the layer removed from the surface of a PCTD highly depends on the soaking time and EDA concentration; it increases as the EDA concentration increases up to for example 700 µm after 2 h of soaking in the EDA solution. After ∼10 min of soaking duration at any of the above-stated concentrations, the BGTD reaches its minimum value, a value which differs from concentration to concentration. An EDA concentration of 85 % in water provided the lowest BGTD of 64.06 ± 3.12 tracks cm - 2 ; ∼6 times lower than that of its original value. It is shown that the layer removal process does not change the registration characteristics of the PCTD and its appearance significantly. The MDL of the PCTDs depends strongly on the BGTD. The MDL values for a desired confidence level were also studied by three calculation methods. The results of the BGTD and the MDL studies under different conditions applied are presented and discussed. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Development and validation of microsatellite markers for Brachiaria ruziziensis obtained by partial genome assembly of Illumina single-end reads

PubMed Central

2013-01-01

Background Brachiaria ruziziensis is one of the most important forage species planted in the tropics. The application of genomic tools to aid the selection of superior genotypes can provide support to B. ruziziensis breeding programs. However, there is a complete lack of information about the B. ruziziensis genome. Also, the availability of genomic tools, such as molecular markers, to support B. ruziziensis breeding programs is rather limited. Recently, next-generation sequencing technologies have been applied to generate sequence data for the identification of microsatellite regions and primer design. In this study, we present a first validated set of SSR markers for Brachiaria ruziziensis, selected from a de novo partial genome assembly of single-end Illumina reads. Results A total of 85,567 perfect microsatellite loci were detected in contigs with a minimum 10X coverage. We selected a set of 500 microsatellite loci identified in contigs with minimum 100X coverage for primer design and synthesis, and tested a subset of 269 primer pairs, 198 of which were polymorphic on 11 representative B. ruziziensis accessions. Descriptive statistics for these primer pairs are presented, as well as estimates of marker transferability to other relevant brachiaria species. Finally, a set of 11 multiplex panels containing the 30 most informative markers was validated and proposed for B. ruziziensis genetic analysis. Conclusions We show that the detection and development of microsatellite markers from genome assembled Illumina single-end DNA sequences is highly efficient. The developed markers are readily suitable for genetic analysis and marker assisted selection of Brachiaria ruziziensis. The use of this approach for microsatellite marker development is promising for species with limited genomic information, whose breeding programs would benefit from the use of genomic tools. To our knowledge, this is the first set of microsatellite markers developed for this important species. PMID:23324172

40 CFR Figure C-4 to Subpart C of... - Illustration of the Minimum Limits for Correlation Coefficient for PM 2.5 and PM 10-2,5 Class II...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 6 2013-07-01 2013-07-01 false Illustration of the Minimum Limits for Correlation Coefficient for PM 2.5 and PM 10-2,5 Class II and III Methods C Figure C-4 to Subpart C of Part 53... Methods and Reference Methods Pt. 53, Subpt. C, Fig. C-4 Figure C-4 to Subpart C of Part 53—Illustration...

40 CFR Figure C-4 to Subpart C of... - Illustration of the Minimum Limits for Correlation Coefficient for PM 2.5 and PM 10-2.5 Class II...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 6 2014-07-01 2014-07-01 false Illustration of the Minimum Limits for Correlation Coefficient for PM 2.5 and PM 10-2.5 Class II and III Methods C Figure C-4 to Subpart C of Part 53... Methods and Reference Methods Pt. 53, Subpt. C, Fig. C-4 Figure C-4 to Subpart C of Part 53—Illustration...

[Determination a variety of acidic gas in air of workplace by Ion Chromatography].

PubMed

Li, Shiyong

2014-10-01

To establish a method for determination of a variety of acid gas in the workplace air by Ion Chromatography. (hydrofluoric acid, hydrogen chloride or hydrochloric acid, sulfur anhydride or sulfuric acid, phosphoric acid, oxalic acid). The sample in workplace air was collected by the porous glass plate absorption tube containing 5 ml leacheate. (Sulfuric acid fog, phosphoric acid aerosol microporous membrane after collection, eluted with 5 ml of eluent.) To separated by AS14+AG14 chromatography column, by carbonate (2.0+1.0) mmol/L (Na(2)CO(3)-NaHCO(3)) as eluent, flow rate of 1 ml/min, then analyzed by electrical conductivity detector. The retain time was used for qualitative and the peak area was used for quantitation. The each ion of a variety of acid gas in the air of workplace were excellent in carbonate eluent separation. The linear range of working curve of 0∼20 mg/L. The correlation coefficient r>0.999; lower detection limit of 3.6∼115 µg/L; quantitative limit of 0.012∼0.53 mg/L; acquisition of 15L air were measured, the minimum detection concentration is 0.004 0∼0.13 mg/m(3). The recovery rate is 99.7%∼101.1%. In the sample without mutual interference ions. Samples stored at room temperature for 7 days. The same analysis method, the detection of various acidic gases in the air of workplace, simple operation, good separation effect, high sensitivity, high detection efficiency, easy popularization and application.

Analysis of small-bowel capsule endoscopy reading by using Quickview mode: training assistants for reading may produce a high diagnostic yield and save time for physicians.

PubMed

Shiotani, Akiko; Honda, Keisuke; Kawakami, Makiko; Kimura, Yoshiki; Yamanaka, Yoshiyuki; Fujita, Minoru; Matsumoto, Hiroshi; Tarumi, Ken-ichi; Manabe, Noriaki; Haruma, Ken

2012-01-01

The aim was to investigate the clinical utility of RAPID Access 6.5 Quickview software and to evaluate whether preview of the capsule endoscopy video by a trained nurse could detect significant lesions accurately compared with endoscopists. As reading capsule endoscopy is time consuming, one possible cost-effective strategy could be the use of trained nonphysicians or newly available software to preread and identify potentially important capsule images. The 100 capsule images of a variety of significant lesions from 87 patients were investigated. The minimum percentages for settings of sensitivity that could pick up the selected images and the detection rate for significant lesions by a well-trained nurse, two endoscopists with limited experience in reading, and one well-trained physician were examined. The frequency of the selected lesions picked up by Quickview mode using percentages for sensitivity settings of 5%, 15%, 25%, and 35% were 61%, 74%, 93%, and 98%, respectively. The percentages for sensitivity significantly correlated (r=0.78, P<0.001) with the reading time. The detection rate by the nurse or the well-trained physician was significantly higher than that by the physician with limited capsule experience (87% and 84.1% vs. 62.7%; P<0.01). The clinical use of Quickview at 25% did not significantly improve the detection rate. Quickview mode can reduce reading time but has an unacceptably miss rate for potentially important lesions. Use of a trained nonphysician assistant can reduce physician's time and improve diagnostic yield.

Rate based failure detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Brett Emery Trabun; Gamage, Thoshitha Thanushka; Bakken, David Edward

This disclosure describes, in part, a system management component and failure detection component for use in a power grid data network to identify anomalies within the network and systematically adjust the quality of service of data published by publishers and subscribed to by subscribers within the network. In one implementation, subscribers may identify a desired data rate, a minimum acceptable data rate, desired latency, minimum acceptable latency and a priority for each subscription. The failure detection component may identify an anomaly within the network and a source of the anomaly. Based on the identified anomaly, data rates and or datamore » paths may be adjusted in real-time to ensure that the power grid data network does not become overloaded and/or fail.« less

Residual toxicity of Cypermethrin in the larvae of coconut pest Oryctes rhinoceros (Coleoptera: Scarabaeidae).

PubMed

Venkatarajappa, P

2001-01-01

The toxic effect of Cypermethrin 10 EC (0.125, 0.25 and 0.5%) was estimated in the bodywall and digestive system of the larvae of Oryctes rhinoceros by HPLC after exposing them to different concentrations (0.125, 0.25 and 0.5%). Among the various concentrations used maximum residues were detected in bodywall (0.25%), whereas at higher concentration (0.5%) the residue detected was minimum. The treatment of Cypermethrin was found to be highly toxic upto 12 h of treatment, after which it declined reaching the minimum by 24 h. The residue of Cypermethrin could not be detected in digestive system. The experiments indicate the pesticide get concentrated in the bodywall to a maximum extent.

Efficiency calibration and minimum detectable activity concentration of a real-time UAV airborne sensor system with two gamma spectrometers.

PubMed

Tang, Xiao-Bin; Meng, Jia; Wang, Peng; Cao, Ye; Huang, Xi; Wen, Liang-Sheng; Chen, Da

2016-04-01

A small-sized UAV (NH-UAV) airborne system with two gamma spectrometers (LaBr3 detector and HPGe detector) was developed to monitor activity concentration in serious nuclear accidents, such as the Fukushima nuclear accident. The efficiency calibration and determination of minimum detectable activity concentration (MDAC) of the specific system were studied by MC simulations at different flight altitudes, different horizontal distances from the detection position to the source term center and different source term sizes. Both air and ground radiation were considered in the models. The results obtained may provide instructive suggestions for in-situ radioactivity measurements of NH-UAV. Copyright © 2016 Elsevier Ltd. All rights reserved.

40 CFR Table 6 to Subpart Dddd of... - Model Rule-Emission Limitations That Apply to Incinerators on and After [Date to be specified in...

Code of Federal Regulations, 2012 CFR

2012-07-01

... per million dry volume 3-run average (1 hour minimum sample time per run) Performance test (Method 10... (Reapproved 2008) c. Oxides of nitrogen 53 parts per million dry volume 3-run average (1 hour minimum sample... average (1 hour minimum sample time per run) Performance test (Method 6 or 6c at 40 CFR part 60, appendix...

40 CFR Table 6 to Subpart Dddd of... - Model Rule-Emission Limitations That Apply to Incinerators on and After [Date to be specified in...

Code of Federal Regulations, 2011 CFR

2011-07-01

... per million dry volume 3-run average (1 hour minimum sample time per run) Performance test (Method 10... (Reapproved 2008) c. Oxides of nitrogen 53 parts per million dry volume 3-run average (1 hour minimum sample... average (1 hour minimum sample time per run) Performance test (Method 6 or 6c at 40 CFR part 60, appendix...

40 CFR Table 2 to Subpart Dddd of... - Model Rule-Emission Limitations That Apply to Incinerators Before [Date to be specified in state...

Code of Federal Regulations, 2014 CFR

2014-07-01

... parts per million by dry volume 3-run average (1 hour minimum sample time per run) Performance test..., appendix A-4). Oxides of nitrogen 388 parts per million by dry volume 3-run average (1 hour minimum sample... (1 hour minimum sample time per run) Performance test (Method 6 or 6c of appendix A of this part) a...

40 CFR Table 2 to Subpart Dddd of... - Model Rule-Emission Limitations That Apply to Incinerators Before [Date to be specified in state...

Code of Federal Regulations, 2013 CFR

2013-07-01

... parts per million by dry volume 3-run average (1 hour minimum sample time per run) Performance test..., appendix A-4). Oxides of nitrogen 388 parts per million by dry volume 3-run average (1 hour minimum sample... (1 hour minimum sample time per run) Performance test (Method 6 or 6c of appendix A of this part) a...

Brief Report: Investigating Uncertainty in the Minimum Mortality Temperature: Methods and Application to 52 Spanish Cities.

PubMed

Tobías, Aurelio; Armstrong, Ben; Gasparrini, Antonio

2017-01-01

The minimum mortality temperature from J- or U-shaped curves varies across cities with different climates. This variation conveys information on adaptation, but ability to characterize is limited by the absence of a method to describe uncertainty in estimated minimum mortality temperatures. We propose an approximate parametric bootstrap estimator of confidence interval (CI) and standard error (SE) for the minimum mortality temperature from a temperature-mortality shape estimated by splines. The coverage of the estimated CIs was close to nominal value (95%) in the datasets simulated, although SEs were slightly high. Applying the method to 52 Spanish provincial capital cities showed larger minimum mortality temperatures in hotter cities, rising almost exactly at the same rate as annual mean temperature. The method proposed for computing CIs and SEs for minimums from spline curves allows comparing minimum mortality temperatures in different cities and investigating their associations with climate properly, allowing for estimation uncertainty.

Capacities of quantum amplifier channels

NASA Astrophysics Data System (ADS)

Qi, Haoyu; Wilde, Mark M.

2017-01-01

Quantum amplifier channels are at the core of several physical processes. Not only do they model the optical process of spontaneous parametric down-conversion, but the transformation corresponding to an amplifier channel also describes the physics of the dynamical Casimir effect in superconducting circuits, the Unruh effect, and Hawking radiation. Here we study the communication capabilities of quantum amplifier channels. Invoking a recently established minimum output-entropy theorem for single-mode phase-insensitive Gaussian channels, we determine capacities of quantum-limited amplifier channels in three different scenarios. First, we establish the capacities of quantum-limited amplifier channels for one of the most general communication tasks, characterized by the trade-off between classical communication, quantum communication, and entanglement generation or consumption. Second, we establish capacities of quantum-limited amplifier channels for the trade-off between public classical communication, private classical communication, and secret key generation. Third, we determine the capacity region for a broadcast channel induced by the quantum-limited amplifier channel, and we also show that a fully quantum strategy outperforms those achieved by classical coherent-detection strategies. In all three scenarios, we find that the capacities significantly outperform communication rates achieved with a naive time-sharing strategy.

The effect of recording and analysis bandwidth on acoustic identification of delphinid species.

PubMed

Oswald, Julie N; Rankin, Shannon; Barlow, Jay

2004-11-01

Because many cetacean species produce characteristic calls that propagate well under water, acoustic techniques can be used to detect and identify them. The ability to identify cetaceans to species using acoustic methods varies and may be affected by recording and analysis bandwidth. To examine the effect of bandwidth on species identification, whistles were recorded from four delphinid species (Delphinus delphis, Stenella attenuata, S. coeruleoalba, and S. longirostris) in the eastern tropical Pacific ocean. Four spectrograms, each with a different upper frequency limit (20, 24, 30, and 40 kHz), were created for each whistle (n = 484). Eight variables (beginning, ending, minimum, and maximum frequency; duration; number of inflection points; number of steps; and presence/absence of harmonics) were measured from the fundamental frequency of each whistle. The whistle repertoires of all four species contained fundamental frequencies extending above 20 kHz. Overall correct classification using discriminant function analysis ranged from 30% for the 20-kHz upper frequency limit data to 37% for the 40-kHz upper frequency limit data. For the four species included in this study, an upper bandwidth limit of at least 24 kHz is required for an accurate representation of fundamental whistle contours.

Pre-main Sequence Evolution and the Hydrogen-Burning Minimum Mass

NASA Astrophysics Data System (ADS)

Nakano, Takenori

There is a lower limit to the mass of the main-sequence stars (the hydrogen-burning minimum mass) below which the stars cannot replenish the energy lost from their surfaces with the energy released by the hydrogen burning in their cores. This is caused by the electron degeneracy in the stars which suppresses the increase of the central temperature with contraction. To find out the lower limit we need the accurate knowledge of the pre-main sequence evolution of very low-mass stars in which the effect of electron degeneracy is important. We review how Hayashi and Nakano (1963) carried out the first determination of this limit.

Modeling of the minimum variable blank holder force based on forming limit diagram (FLD) in deep drawing process

NASA Astrophysics Data System (ADS)

Candra, S.; Batan, I. M. L.; Berata, W.; Pramono, A. S.

2017-11-01

This paper presents the mathematical approach of minimum blank holder force to prevent wrinkling in deep drawing process of the cylindrical cup. Based on the maximum of minor-major strain ratio, the slab method was applied to determine the modeling of minimum variable blank holder force (VBHF) and it compared to FE simulation. The Tin steel sheet of T4-CA grade, with the thickness of 0.2 mm was used in this study. The modeling of minimum VBHF can be used as a simple reference to prevent wrinkling in deep drawing.

Detection of atrial fibrillation after ischemic stroke or transient ischemic attack: a systematic review and meta-analysis.

PubMed

Kishore, Amit; Vail, Andy; Majid, Arshad; Dawson, Jesse; Lees, Kennedy R; Tyrrell, Pippa J; Smith, Craig J

2014-02-01

Atrial fibrillation (AF) confers a high risk of recurrent stroke, although detection methods and definitions of paroxysmal AF during screening vary. We therefore undertook a systematic review and meta-analysis to determine the frequency of newly detected AF using noninvasive or invasive cardiac monitoring after ischemic stroke or transient ischemic attack. Prospective observational studies or randomized controlled trials of patients with ischemic stroke, transient ischemic attack, or both, who underwent any cardiac monitoring for a minimum of 12 hours, were included after electronic searches of multiple databases. The primary outcome was detection of any new AF during the monitoring period. We prespecified subgroup analysis of selected (prescreened or cryptogenic) versus unselected patients and according to duration of monitoring. A total of 32 studies were analyzed. The overall detection rate of any AF was 11.5% (95% confidence interval, 8.9%-14.3%), although the timing, duration, method of monitoring, and reporting of diagnostic criteria used for paroxysmal AF varied. Detection rates were higher in selected (13.4%; 95% confidence interval, 9.0%-18.4%) than in unselected patients (6.2%; 95% confidence interval, 4.4%-8.3%). There was substantial heterogeneity even within specified subgroups. Detection of AF was highly variable, and the review was limited by small sample sizes and marked heterogeneity. Further studies are required to inform patient selection, optimal timing, methods, and duration of monitoring for detection of AF/paroxysmal AF.

Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano- and micro-sized magnetic beads.

PubMed

Uddin, Rokon; Burger, Robert; Donolato, Marco; Fock, Jeppe; Creagh, Michael; Hansen, Mikkel Fougt; Boisen, Anja

2016-11-15

We present a biosensing platform for the detection of proteins based on agglutination of aptamer coated magnetic nano- or microbeads. The assay, from sample to answer, is integrated on an automated, low-cost microfluidic disc platform. This ensures fast and reliable results due to a minimum of manual steps involved. The detection of the target protein was achieved in two ways: (1) optomagnetic readout using magnetic nanobeads (MNBs); (2) optical imaging using magnetic microbeads (MMBs). The optomagnetic readout of agglutination is based on optical measurement of the dynamics of MNB aggregates whereas the imaging method is based on direct visualization and quantification of the average size of MMB aggregates. By enhancing magnetic particle agglutination via application of strong magnetic field pulses, we obtained identical limits of detection of 25pM with the same sample-to-answer time (15min 30s) using the two differently sized beads for the two detection methods. In both cases a sample volume of only 10µl is required. The demonstrated automation, low sample-to-answer time and portability of both detection instruments as well as integration of the assay on a low-cost disc are important steps for the implementation of these as portable tools in an out-of-lab setting. Copyright © 2016 Elsevier B.V. All rights reserved.

Methodological Considerations When Quantifying High-Intensity Efforts in Team Sport Using Global Positioning System Technology.

PubMed

Varley, Matthew C; Jaspers, Arne; Helsen, Werner F; Malone, James J

2017-09-01

Sprints and accelerations are popular performance indicators in applied sport. The methods used to define these efforts using athlete-tracking technology could affect the number of efforts reported. This study aimed to determine the influence of different techniques and settings for detecting high-intensity efforts using global positioning system (GPS) data. Velocity and acceleration data from a professional soccer match were recorded via 10-Hz GPS. Velocity data were filtered using either a median or an exponential filter. Acceleration data were derived from velocity data over a 0.2-s time interval (with and without an exponential filter applied) and a 0.3-second time interval. High-speed-running (≥4.17 m/s 2 ), sprint (≥7.00 m/s 2 ), and acceleration (≥2.78 m/s 2 ) efforts were then identified using minimum-effort durations (0.1-0.9 s) to assess differences in the total number of efforts reported. Different velocity-filtering methods resulted in small to moderate differences (effect size [ES] 0.28-1.09) in the number of high-speed-running and sprint efforts detected when minimum duration was <0.5 s and small to very large differences (ES -5.69 to 0.26) in the number of accelerations when minimum duration was <0.7 s. There was an exponential decline in the number of all efforts as minimum duration increased, regardless of filtering method, with the largest declines in acceleration efforts. Filtering techniques and minimum durations substantially affect the number of high-speed-running, sprint, and acceleration efforts detected with GPS. Changes to how high-intensity efforts are defined affect reported data. Therefore, consistency in data processing is advised.

Method to improve reliability of a fuel cell system using low performance cell detection at low power operation

DOEpatents

Choi, Tayoung; Ganapathy, Sriram; Jung, Jaehak; Savage, David R.; Lakshmanan, Balasubramanian; Vecasey, Pamela M.

2013-04-16

A system and method for detecting a low performing cell in a fuel cell stack using measured cell voltages. The method includes determining that the fuel cell stack is running, the stack coolant temperature is above a certain temperature and the stack current density is within a relatively low power range. The method further includes calculating the average cell voltage, and determining whether the difference between the average cell voltage and the minimum cell voltage is greater than a predetermined threshold. If the difference between the average cell voltage and the minimum cell voltage is greater than the predetermined threshold and the minimum cell voltage is less than another predetermined threshold, then the method increments a low performing cell timer. A ratio of the low performing cell timer and a system run timer is calculated to identify a low performing cell.

28 CFR 544.74 - Work assignment limitations.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Work assignment limitations. 544.74... EDUCATION Literacy Program § 544.74 Work assignment limitations. These limitations on work assignment... order to be considered for a commissary work assignment above minimum pay level, an institution work...

Degree Day Requirements for Kudzu Bug (Hemiptera: Plataspidae), a Pest of Soybeans.

PubMed

Grant, Jessica I; Lamp, William O

2018-04-02

Understanding the phenology of a new potential pest is fundamental for the development of a management program. Megacopta cribraria Fabricius (Hemiptera: Plataspidae), kudzu bug, is a pest of soybeans first detected in the United States in 2009 and in Maryland in 2013. We observed the phenology of kudzu bug life stages in Maryland, created a Celsius degree-day (CDD) model for development, and characterized the difference between microhabitat and ambient temperatures of both kudzu, Pueraria montana (Lour.) Merr. (Fabales: Fabaceae) and soybeans, Glycine max (L.) Merrill (Fabales: Fabaceae). In 2014, low population numbers yielded limited resolution from field phenology observations. We observed kudzu bug populations persisting within Maryland; but between 2013 and 2016, populations were low compared to populations in the southeastern United States. Based on the degree-day model, kudzu bug eggs require 80 CDD at a minimum temperature of 14°C to hatch. Nymphs require 545 CDD with a minimum temperature of 16°C for development. The CDD model matches field observations when factoring a biofix date of April 1 and a minimum preoviposition period of 17 d. The model suggests two full generations per year in Maryland. Standard air temperature monitors do not affect model predictions for pest management, as microhabitat temperature differences did not show a clear trend between kudzu and soybeans. Ultimately, producers can predict the timing of kudzu bug life stages with the CDD model for the use of timing management plans in soybean fields.

Possible space weather influence on the Earth wheat prices

NASA Astrophysics Data System (ADS)

Pustil'Nik, L.; Yom Din, G.; Dorman, L.

We present development of our study of possible influence of space weather modulated by cycle of solar activity on the price bursts in the Earth markets In our previous works 1 2 we showed that correspondent response may have place in the specific locations characterized by a high sensitivity of the weather cloudiness in particular to cosmic ray variation b risk zone agriculture c isolated wheat market with limited external supply of agriculture production We showed that in this situation we may wait specific price burst reaction on unfavorable phase of solar activity and space weather what lead to corresponding abnormalities in the local weather and next crop failure We showed that main types of manifestation of this connection are a Distribution of intervals between price bursts must be like to the distribution of intervals between correspondent extremes of solar activity minimums or maximums b price asymmetry between opposite states of solar activity price in the one type of activity state is systematically higher then in the opposite one We showed in our previous publications that this influence in interval distribution is detected with high reliability in Mediaeval England 1250-1700 both for wheat prices and price of consumables basket We showed that for period of Maunder Minimum price asymmetry of wheat prices observed all prices in minimum state of solar activity was higher the prices in the next maximum state We showed later that this price asymmetry had place in 20-th century in USA durum prices too In

A six hundred-year annual minimum temperature history for the central Tibetan Plateau derived from tree-ring width series

NASA Astrophysics Data System (ADS)

He, Minhui; Yang, Bao; Datsenko, Nina M.

2014-08-01

The recent unprecedented warming found in different regions has aroused much attention in the past years. How temperature has really changed on the Tibetan Plateau (TP) remains unknown since very limited high-resolution temperature series can be found over this region, where large areas of snow and ice exist. Herein, we develop two Juniperus tibetica Kom. tree-ring width chronologies from different elevations. We found that the two tree-ring series only share high-frequency variability. Correlation, response function and partial correlation analysis indicate that prior year annual (January-December) minimum temperature is most responsible for the higher belt juniper radial growth, while more or less precipitation signal is contained by the tree-ring width chronology at the lower belt and is thus excluded from further analysis. The tree growth-climate model accounted for 40 % of the total variance in actual temperature during the common period 1957-2010. The detected temperature signal is further robustly verified by other results. Consequently, a six century long annual minimum temperature history was firstly recovered for the Yushu region, central TP. Interestingly, the rapid warming trend during the past five decades is identified as a significant cold phase in the context of the past 600 years. The recovered temperature series reflects low-frequency variability consistent with other temperature reconstructions over the whole TP region. Furthermore, the present recovered temperature series is associated with the Asian monsoon strength on decadal to multidecadal scales over the past 600 years.

Target Coverage in Wireless Sensor Networks with Probabilistic Sensors

PubMed Central

Shan, Anxing; Xu, Xianghua; Cheng, Zongmao

2016-01-01

Sensing coverage is a fundamental problem in wireless sensor networks (WSNs), which has attracted considerable attention. Conventional research on this topic focuses on the 0/1 coverage model, which is only a coarse approximation to the practical sensing model. In this paper, we study the target coverage problem, where the objective is to find the least number of sensor nodes in randomly-deployed WSNs based on the probabilistic sensing model. We analyze the joint detection probability of target with multiple sensors. Based on the theoretical analysis of the detection probability, we formulate the minimum ϵ-detection coverage problem. We prove that the minimum ϵ-detection coverage problem is NP-hard and present an approximation algorithm called the Probabilistic Sensor Coverage Algorithm (PSCA) with provable approximation ratios. To evaluate our design, we analyze the performance of PSCA theoretically and also perform extensive simulations to demonstrate the effectiveness of our proposed algorithm. PMID:27618902

A Methodology to Assess the Capability of Engine Designs to Meet Closed-Loop Performance and Operability Requirements

NASA Technical Reports Server (NTRS)

Zinnecker, Alicia M.; Csank, Jeffrey

2015-01-01

Designing a closed-loop controller for an engine requires balancing trade-offs between performance and operability of the system. One such trade-off is the relationship between the 95 percent response time and minimum high-pressure compressor (HPC) surge margin (SM) attained during acceleration from idle to takeoff power. Assuming a controller has been designed to meet some specification on response time and minimum HPC SM for a mid-life (nominal) engine, there is no guarantee that these limits will not be violated as the engine ages, particularly as it reaches the end of its life. A characterization for the uncertainty in this closed-loop system due to aging is proposed that defines elliptical boundaries to estimate worst-case performance levels for a given control design point. The results of this characterization can be used to identify limiting design points that bound the possible controller designs yielding transient results that do not exceed specified limits in response time or minimum HPC SM. This characterization involves performing Monte Carlo simulation of the closed-loop system with controller constructed for a set of trial design points and developing curve fits to describe the size and orientation of each ellipse; a binary search procedure is then employed that uses these fits to identify the limiting design point. The method is demonstrated through application to a generic turbofan engine model in closed-loop with a simplified controller; it is found that the limit for which each controller was designed was exceeded by less than 4.76 percent. Extension of the characterization to another trade-off, that between the maximum high-pressure turbine (HPT) entrance temperature and minimum HPC SM, showed even better results: the maximum HPT temperature was estimated within 0.76 percent. Because of the accuracy in this estimation, this suggests another limit that may be taken into consideration during design and analysis. It also demonstrates the extension of the characterization to other attributes that contribute to the performance or operability of the engine. Metrics are proposed that, together, provide information on the shape of the trade-off between response time and minimum HPC SM, and how much each varies throughout the life cycle, at the limiting design points. These metrics also facilitate comparison of the expected transient behavior for multiple engine models.

A Methodology to Assess the Capability of Engine Designs to Meet Closed-loop Performance and Operability Requirements

NASA Technical Reports Server (NTRS)

Zinnecker, Alicia M.; Csank, Jeffrey T.

2015-01-01

Designing a closed-loop controller for an engine requires balancing trade-offs between performance and operability of the system. One such trade-off is the relationship between the 95% response time and minimum high-pressure compressor (HPC) surge margin (SM) attained during acceleration from idle to takeoff power. Assuming a controller has been designed to meet some specification on response time and minimum HPC SM for a mid-life (nominal) engine, there is no guarantee that these limits will not be violated as the engine ages, particularly as it reaches the end of its life. A characterization for the uncertainty in this closed-loop system due to aging is proposed that defines elliptical boundaries to estimate worst-case performance levels for a given control design point. The results of this characterization can be used to identify limiting design points that bound the possible con- troller designs yielding transient results that do not exceed specified limits in response time or minimum HPC SM. This characterization involves performing Monte Carlo simulation of the closed-loop system with controller constructed for a set of trial design points and developing curve fits to describe the size and orientation of each ellipse; a binary search procedure is then employed that uses these fits to identify the limiting design point. The method is demonstrated through application to a generic turbofan engine model in closed- loop with a simplified controller; it is found that the limit for which each controller was designed was exceeded by less than 4.76%. Extension of the characterization to another trade-off, that between the maximum high-pressure turbine (HPT) entrance temperature and minimum HPC SM, showed even better results: the maximum HPT temperature was estimated within 0.76%. Because of the accuracy in this estimation, this suggests another limit that may be taken into consideration during design and analysis. It also demonstrates the extension of the characterization to other attributes that contribute to the performance or operability of the engine. Metrics are proposed that, together, provide information on the shape of the trade-off between response time and minimum HPC SM, and how much each varies throughout the life cycle, at the limiting design points. These metrics also facilitate comparison of the expected transient behavior for multiple engine models.

A Pair Production Telescope for Medium-Energy Gamma-Ray Polarimetry

NASA Technical Reports Server (NTRS)

Hunter, Stanley D.; Bloser, Peter F.; Depaola, Gerardo; Dion, Michael P.; DeNolfo, Georgia A.; Hanu, Andrei; Iparraguirre, Marcos; Legere, Jason; Longo, Francesco; McConnell, Mark L.;

Compact SOI optimized slot microring coupled phase-shifted Bragg grating resonator for sensing

NASA Astrophysics Data System (ADS)

Zhao, Chao Ying; Zhang, Lei; Zhang, Cheng Mei

2018-05-01

We propose a novel sensor structure composed of a slot microring and a phase-shifted sidewall Bragg gratings in a slot waveguide. We first present a theoretical analysis of transmission by using the transfer matrix. Then, the mode-field distributions of transmission spectrum obtained from 3D simulations based on FDTD method demonstrates that our sensor exhibit theoretical sensitivity of 297 . 13 nm / RIU, a minimum detection limit of 1 . 1 × 10-4 RIU, the maximum extinction ratio of 20 dB, the quality factor of 2 × 103 and a compact dimension-theoretical structure of 15 μm × 8 . 5 μm. Finally, the sensor's performance is simulated for NaCl solution.

The uses of synchrotron radiation sources for elemental and chemical microanalysis

USGS Publications Warehouse

Chen, J.R.; Chao, E.C.T.; Minkin, J.A.; Back, J.M.; Jones, K.W.; Rivers, M.L.; Sutton, S.R.

1990-01-01

Synchrotron radiation sources offer important features for the analysis of a material. Among these features is the ability to determine both the elemental composition of the material and the chemical state of its elements. For microscopic analysis synchrotron X-ray fluorescence (SXRF) microprobes now offer spatial resolutions of 10 ??m with minimum detection limits in the 1-10 ppm range depending on the nature of the sample and the synchrotron source used. This paper describes the properties of synchrotron radiation and their importance for elemental analysis, existing synchrotron facilities and those under construction that are optimum for SXRF microanalysis, and a number of applications including the high energy excitation of the K lines of heavy elements, microtomography, and XANES and EXAFS spectroscopies. ?? 1990.

Supercritical fluid extraction and direct fluid injection mass spectrometry for the determination of trichothecene mycotoxins in wheat samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kalinoski, H.T.; Udseth, H.R.; Wright, B.W.

1986-10-01

The application of on-line supercritical fluid extraction with chemical ionization mass spectrometry and collision induced dissociation tandem mass spectrometry for the rapid identification of parts-per-million levels of several trichothecene mycotoxins is demonstrated. Supercritical carbon dioxide is shown to allow identification of mycotoxins with minimum sample handling in complex natural matrices (e.g., wheat). Tandem mass spectrometry techniques are employed for unambiguous identification of compounds of varying polarity, and false positives from isobaric compounds are avoided. Capillary column supercritical fluid chromatography-mass spectrometry of a supercritical fluid extract of the same sample was also performed and detection limits in the parts-per-billion range appearmore » feasible.« less

First Results of the LUX Dark Matter Experiment

NASA Astrophysics Data System (ADS)

Carmona-Benitez, M. C.; Akerib, D. S.; Araújo, H. M.; Bai, X.; Bailey, A. J.; Balajthy, J.; Beltrame, P.; Bernard, E.; Bernstein, A.; Bradley, A.; Byram, D.; Cahn, S. B.; Chan, C.; Chapman, J. J.; Chiller, A. A.; Chiller, C.; Currie, A.; de Viveiros, L.; Dobi, A.; Dobson, J.; Druszkiewicz, E.; Edwards, B.; Faham, C. H.; Fiorucci, S.; Flores, C.; Gaitskell, R. J.; Gehman, V. M.; Ghag, C.; Gibson, K. R.; Gilchriese, M. G. D.; Hall, C.; Hanhardt, M.; Haselschwardt, S.; Hertel, S. A.; Horn, M.; Huang, D. Q.; Ihm, M.; Jacobsen, R. G.; Kazkaz, K.; Knoche, R.; Larsen, N. A.; Lee, C.; Lenardo, B.; Lesko, K. T.; Lindote, A.; Lopes, M. I.; Malling, D. C.; Manalaysay, A.; Mannino, R.; McKinsey, D. N.; Mei, D.-M.; Mock, J.; Moongweluwan, M.; Morad, J.; Murphy, A. St. J.; Nehrkorn, C.; Nelson, H.; Neves, F.; Ott, R. A.; Pangilinan, M.; Parker, P. D.; Pease, E. K.; Pech, K.; Phelps, P.; Reichhart, L.; Shutt, T.; Silva, C.; Solovov, V. N.; Sorensen, P.; O'Sullivan, K.; Sumner, T. J.; Szydagis, M.; Taylor, D.; Tennyson, B.; Tiedt, D. R.; Tripathi, M.; Tvrznikova, L.; Uvarov, S.; Verbus, J. R.; Walsh, N.; Webb, R.; White, J. T.; Witherell, M. S.; Wolfs, F. L. H.; Woods, M.; Zhang, C.; LUX Collaboration

2016-04-01

LUX (Large Underground Xenon) is a dark matter direct detection experiment deployed at the 4850' level of the Sanford Underground Research Facility (SURF) in Lead, SD, operating a 370 kg dual-phase xenon TPC. Results of the first WIMP search run were presented in late 2013, for the analysis of 85.3 live-days with a fiducial volume of 118 kg, taken during the period of April to August 2013. The experiment exhibited a sensitivity to spin-independent WIMP-nucleon elastic scattering with a minimum upper limit on the cross section of 7.6 ×10-46cm2 at a WIMP mass of 33 GeV/c2, becoming the world's leading WIMP search result, in conflict with several previous claimed hints of discovery.

Minimizing Higgs potentials via numerical polynomial homotopy continuation

NASA Astrophysics Data System (ADS)

Maniatis, M.; Mehta, D.

2012-08-01

The study of models with extended Higgs sectors requires to minimize the corresponding Higgs potentials, which is in general very difficult. Here, we apply a recently developed method, called numerical polynomial homotopy continuation (NPHC), which guarantees to find all the stationary points of the Higgs potentials with polynomial-like non-linearity. The detection of all stationary points reveals the structure of the potential with maxima, metastable minima, saddle points besides the global minimum. We apply the NPHC method to the most general Higgs potential having two complex Higgs-boson doublets and up to five real Higgs-boson singlets. Moreover the method is applicable to even more involved potentials. Hence the NPHC method allows to go far beyond the limits of the Gröbner basis approach.

Primeval galaxies in the sub-mm and mm

NASA Technical Reports Server (NTRS)

Bond, J. Richard; Myers, Steven T.

1993-01-01

Although the results of COBE's FIRAS experiment 1 constrain the deviation in energy from the CMB blackbody in the 500-5000 micron range to be delta E/E, sub cmb less than 0.005, primeval galaxies can still lead to a brilliant sub-mm sky of non-Gaussian sources that are detectable at 10 inch resolution from planned arrays such as SCUBA on the James Clerk Maxwell Telescope and, quite plausibly, at sub-arcsecond resolution in planned mm and sub-mm interferometers. Here, we apply our hierarchical peaks method to a CDM model to construct sub-mm and mm maps of bursting PG's appropriate for these instruments with minimum contours chosen to correspond to realistic observational parameters for them and which pass the FIRAS limits.

The Amsterdam quintuplet nuclear microprobe

NASA Astrophysics Data System (ADS)

van den Putte, M. J. J.; van den Brand, J. F. J.; Jamieson, D. N.; Rout, B.; Szymanski, R.

2003-09-01

A new nuclear microprobe comprising of a quintuplet lens system is being constructed at the Ion Beam Facility of the "Vrije Universiteit" Amsterdam in collaboration with the Microanalytical Research Centre of the University of Melbourne. An overview of the Amsterdam set-up will be presented. Detailed characterisation of the individual lenses was performed with the grid shadow method using a 2000 mesh Cu grid mounted at a relative angle of 0.5° to the vertical lens line focus. The lenses were found to have very low parasitic aberrations equal or below the minimum detectable limit for the method, which was approximately 0.1% for the sextupole component and 0.2% for the octupole component. We present experimental and theoretical grid shadow patterns, showing results for all five lenses.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lam, Jessica; Rennick, Christopher J.; Softley, Timothy P.

A chopper wheel construct is used to shorten the duration of a molecular beam to 13 μs. Molecular beams seeded with NO or with Br{sub 2} and an initial pulse width of ≥200 μs were passed through a spinning chopper wheel, which was driven by a brushless DC in vacuo motor at a range of speeds, from 3000 rpm to 80 000 rpm. The resulting duration of the molecular-beam pulses measured at the laser detection volume ranged from 80 μs to 13 μs and was the same for both NO and Br{sub 2}. The duration is consistent with a simple analyticalmore » model, and the minimum pulse width measured is limited by the spreading of the beam between the chopper and the detection point as a consequence of the longitudinal velocity distribution of the beam. The setup adopted here effectively eliminates buildup of background gas without the use of a differential pumping stage, and a clean narrow pulse is obtained with low rotational temperature.« less

Direct Imaging Of Long Period Radial Velocity Targets With NICI

NASA Astrophysics Data System (ADS)

Salter, Graeme S.; Tinney, Chris G.; Wittenmyer, Robert A.; Jenkins, James S.; Jones, Hugh R. A.; O'Toole, Simon J.

2014-01-01

We are finally entering an era where radial velocity and direct imaging parameter spaces are starting to overlap. Radial velocity measurements provide us with a minimum mass for an orbiting companion (the mass as a function of the inclination of the system). By following up these long period radial velocity detections with direct imaging we can determine whether a trend seen is due to an orbiting planet at low inclination or an orbiting brown dwarf at high inclination. In the event of a non-detection we are still able to put a limit on the maximum mass of the orbiting body. The Anglo-Australian Planet Search is one of the longest baseline radial velocity planet searches in existence, amongst its targets are many that show long period trends in the data. Here we present our direct imaging survey of these objects with our results to date. ADI Observations have been made using NICI (Near Infrared Coronagraphic Imager) on Gemini South and analysed using an in house, LOCI-like, post processing.

A Herschel-Resolved Debris Disk Around the Nearby G Star HIP 32480

NASA Technical Reports Server (NTRS)

Stapelfeldt, K.

2011-01-01

The Herschel Space Observatory is providing unprecedented sensitivity and angular resolution in the far-infrared. The DUNES Key Project (DUst around NEarby Stars, PI Carlos Eiroa) has finished its survey of 133 FGK stars within 25 pc of the Sun using the PACS photometer at 100 and 160 microns. We report the detection of a resolved debris ring around HIP 32480, a G0 star 16.5 parsecs distant. The ring is almost 300 AU in diameter and inclined 30 degrees from edge-on. We present a thermal emission model for the system that fits the Spitzer spectroscopy and Herschel images of the system. We find a minimum grainsize of approximately 4 microns in the main ring and a distinct warm dust population interior to it. Faint detached emission features just outside the ring may trace a separate, more distant ring in the system. The non-detection of the ring in archival HST/ACS coronagraphic images limits the dust grain albedo in the ring to be no more than 10%.

A Resolved Debris Disk Around the Nearby G Star HIP 32480

NASA Technical Reports Server (NTRS)

Stapelfeldt, K. R.; Bryden, G. C.; Marshall, J.; Eiroa, C.; Absil, O.; Mora, A.; Krist, J. E.; Su, K. Y. L.

2012-01-01

The Herschel Space Observatory is providing unprecedented sensitivity and angular resolution in the far-infrared. The DUNES Key Project (DUst around NEarby Stars, PI Carlos Eiroa) has finished its survey of 133 FGK stars within 25 pc of the Sun using the PACS photometer at 100 and 160 microns. We report the detection of a resolved debris ring around HIP 32480, a GO star 16.5 parsecs distant. The ring is almost 300 AU in diameter and inclined 30 degrees from edge-on. We present a thermal emission model for the system that fits the Spitzer spectroscopy and Herschel images of the system. We find a minimum grain-size of 4 microns in the main ring and a distinct warm dust population interior to it. Faint detached emission features just outside the ring may trace a separate, more distant ring in the system. The non-detection of the ring in archival HST/ACS coronagraphic images limits the dust grain albedo in the ring to be no more than 10%.

Residue depletion of tilmicosin in chicken tissues.

PubMed

Zhang, Yue; Jiang, Haiyang; Jin, Xingjun; Shen, Zhangqi; Shen, Jianzhong; Fu, Caixia; Guo, Junlin

2004-05-05

A high-performance liquid chromatography (HPLC) method with detection at 290 nm was modified and validated for the determination of tilmicosin residues in broiler chicken tissues. The limits of detection (LOD) of the method were 0.01 microg/g for muscle and 0.025 microg/g for liver and kidney. Average recoveries ranged from 80.4 to 88.3%. Relative standard deviation values ranged from 5.2 to 12.1%. Residue depletion of tilmicosin in broiler chickens was examined after dosing over a 5-day period by incorporation of the drug into drinking water at 37.5 and 75.0 mg/L. Tilmicosin concentrations in liver and kidney were highest on day 3 of medication and on day 5 in muscle, in both low- and high-dose groups. The residue levels in both groups were significantly higher in liver than in kidney or muscle. A minimum withdrawal time of 9 days was indicated for residue levels in muscle, liver, and kidney tissues below the maximum residue level (MRL).

An electrochemical sensor based on nitrogen doped carbon material prepared from nitrogen-containing precursors

NASA Astrophysics Data System (ADS)

Cui, G. Y.; Wang, C. Y.; Xiang, G. Q.; Zhou, B.

2018-01-01

In this work, a nitrogen doped carbon material (NDC) was prepared by using a copper adenine complex as precursor and applied to electrochemical sensing of Vitamin B2 (VB2). The experimental results show that the nitrogen doped carbon material is obtained after calcination at 650 °C under argon atmosphere, afterwards, which were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), fourier transform infrared (FT-IR), and electrochemical method. According to the XRD data, the product was a carbon material, and infrared data demonstrates that there are two kinds of carbonyl nitrogen combination, respectively, C-N and C = N combination of ways. Importantly, we used NDC to construct electrochemical biosensor to detect VB2 by differential pulse voltammetry (DPV). The linear range was 6 × 10-6 - 3.5 × 10-4 M (R=0.9962), the minimum detection limit was 5.4 × 10-6 M, and the sensitivity is better. Consequently, it has better sensing performance.

WDR-PK-AK-018

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hollister, R

2009-08-26

Method - CES SOP-HW-P556 'Field and Bulk Gamma Analysis'. Detector - High-purity germanium, 40% relative efficiency. Calibration - The detector was calibrated on February 8, 2006 using a NIST-traceable sealed source, and the calibration was verified using an independent sealed source. Count Time and Geometry - The sample was counted for 20 minutes at 72 inches from the detector. A lead collimator was used to limit the field-of-view to the region of the sample. The drum was rotated 180 degrees halfway through the count time. Date and Location of Scans - June 1,2006 in Building 235 Room 1136. Spectral Analysismore » Spectra were analyzed with ORTEC GammaVision software. Matrix and geometry corrections were calculated using OR TEC Isotopic software. A background spectrum was measured at the counting location. No man-made radioactivity was observed in the background. Results were determined from the sample spectra without background subtraction. Minimum detectable activities were calculated by the Nureg 4.16 method. Results - Detected Pu-238, Pu-239, Am-241 and Am-243.« less

Optical feedback cavity-enhanced absorption spectroscopy with a 3.24 μm interband cascade laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Manfred, K. M.; Ritchie, G. A. D.; Lang, N.

2015-06-01

The development of interband cascade lasers (ICLs) has made the strong C-H transitions in the 3 μm spectral region increasingly accessible. We present the demonstration of a single mode distributed feedback ICL coupled to a V-shaped optical cavity in an optical feedback cavity-enhanced absorption spectroscopy (OF-CEAS) experiment. We achieved a minimum detectable absorption coefficient, α{sub min}, of (7.1±0.2)×10{sup −8} cm{sup −1} for a spectrum of CH{sub 4} at 3.24 μm with a two second acquisition time (100 scans averaged). This corresponds to a detection limit of 3 ppb CH{sub 4} at atmospheric pressure, which is comparable to previously reported OF-CEAS instruments with diodemore » lasers or quantum cascade lasers. The ability to frequency lock an ICL source in the important 3 μm region to an optical cavity holds great promise for future spectroscopic applications.« less

Occurrence of emerging contaminants in water and bed material in the Missouri River, North Dakota, 2007

USGS Publications Warehouse

Damschen, William C.; Lundgren, Robert F.

2009-01-01

The U.S. Geological Survey (USGS), in cooperation with the Standing Rock Sioux Tribe, conducted a reconnaissance study to determine the occurrence of emerging contaminants in water and bed sediment within the Missouri River upstream and downstream from the cities of Bismarck and Mandan, North Dakota, and upstream from the city of Fort Yates, North Dakota, during September-October 2007. At each site, water samples were collected twice and bed-sediment samples were collected once. Samples were analyzed for more than 200 emerging contaminants grouped into four compound classes - wastewater compounds, human-health pharmaceutical compounds, hormones, and antibiotics. Only sulfamethoxazole, an antibiotic, was present at a concentration higher than minimum detection limits. It was detected in a water sample collected downstream from the cities of Bismarck and Mandan, and in bed-sediment samples collected at the two sites downstream from the cities of Bismarck and Mandan and upstream from Fort Yates. Sulfamethoxazole is an antibiotic commonly used for treating bacterial infections in humans and animals.

Diode laser sensor to monitor HCL in a plasma etch reactor

NASA Astrophysics Data System (ADS)

Kim, Suhong; Klimecky, Pete; Chou, Shang-I.; Jeffries, Jay B.; Terry, Fred L., Jr.; Hanson, Ronald K.

2002-09-01

Absorption measurements of HCl during plasma etching of poly-silicon are made using the P(4) transition in the first vibrational overtone band near 1.79 μm. Single path absorption provides a real-time HCl monitor during etching of six-inch wafers in a commercial Lam Research 9400SE reactor at the University of Michigan. Wavelength modulation at 10.7 MHz is used to distinguish the absorption signal from the strong plasma emission. The laser center frequency is ramp-tuned at 500 Hz providing an HCl measurement every 2ms. Direct absorption measurements without the plasma are used to calibrate the wavelength modulation signal. The minimum detectable absorbance was 5x(10)-6 with 50 ms averaging, leading to an HCl detection limit of ~(10)12cm-3. For a given ratio of the feedstock HBr/Cl2, the measured HCl concentration tracks the average etch rate. These measurements demonstrate the feasibility of a real-time diode laser-based etch rate sensor.

Transducer-based evaluation of tremor

PubMed Central

Haubenberger, Dietrich; Abbruzzese, Giovanni; Bain, Peter G; Bajaj, Nin; Benito-León, Julián; Bhatia, Kailash P; Deuschl, Günther; Forjaz, Maria João; Hallett, Mark; Louis, Elan D; Lyons, Kelly E; Mestre, Tiago A; Raethjen, Jan; Stamelou, Maria; Tan, Eng-King; Testa, Claudia M; Elble, Rodger J

2016-01-01

The Movement Disorder Society (MDS) established a task force on tremor that reviewed the use of transducer-based measures in the quantification and characterization of tremor. Studies of accelerometry, electromyography, activity monitoring, gyroscopy, digitizing tablet-based measures, vocal acoustic analysis, and several other transducer-based methods were identified by searching PubMed.gov. The availability, use, acceptability, reliability, validity, and responsiveness were reviewed for each measure using the following criteria: 1) used in the assessment of tremor, 2) used in published studies by people other than the developers, and 3) adequate clinimetric testing. Accelerometry, gyroscopy, electromyography, and digitizing tablet-based measures fulfilled all three criteria. Compared to rating scales, transducers are far more sensitive to changes in tremor amplitude and frequency, but they do not appear to be more capable of detecting a change that exceeds random variability in tremor amplitude (minimum detectable change). The use of transducer-based measures requires careful attention to their limitations and validity in a particular clinical or research setting. PMID:27273470

Occurrence of perchlorate in drinking water and seawater in South Korea.

PubMed

Her, Namguk; Jeong, Hyunchan; Kim, Jongsung; Yoon, Yeomin

2011-08-01

Concentrations of perchlorate were determined by both liquid-chromatography-mass spectrometry (LC-MS) and ion chromatography tandem mass spectrometry (IC-MS/MS) in 520 tap-water, 48 bottled-water, and 9 seawater samples obtained or purchased from >100 different locations in South Korea. The method detection limits were 0.013 μg/L for LC-MS and 0.005 μg/L for IC-MS/MS, and the limits of quantification (LOQs) were 0.10 μg/L for LC-MS and 0.032 μg/L for IC-MS/MS. Perchlorate was detected in most (80%) of the tap-water samples, with concentrations higher than the LOQ; the concentrations ranged from <1.0 to 6.1 μg/L (mean 0.56). Perchlorate was detected by IC-MS/MS in many (n = 23) of the bottled-water samples, with concentrations higher then the LOQ, ranging from 0.04 to 0.29 μg/L (mean 0.07 ± 0.01). The concentrations of perchlorate in all seawater samples collected from the various locations were higher than the LOQ, with a mean concentration of 1.15 ± 0.01 μg/L (maximum 6.11 and minimum 0.11). This study provides further evidence that drinking-water sources have been contaminated by perchlorate. To the best of our knowledge, this is the first comprehensive study on perchlorate assessment in drinking water and seawater in South Korea.

Minimum viewing angle for visually guided ground speed control in bumblebees.

PubMed

Baird, Emily; Kornfeldt, Torill; Dacke, Marie

2010-05-01

To control flight, flying insects extract information from the pattern of visual motion generated during flight, known as optic flow. To regulate their ground speed, insects such as honeybees and Drosophila hold the rate of optic flow in the axial direction (front-to-back) constant. A consequence of this strategy is that its performance varies with the minimum viewing angle (the deviation from the frontal direction of the longitudinal axis of the insect) at which changes in axial optic flow are detected. The greater this angle, the later changes in the rate of optic flow, caused by changes in the density of the environment, will be detected. The aim of the present study is to examine the mechanisms of ground speed control in bumblebees and to identify the extent of the visual range over which optic flow for ground speed control is measured. Bumblebees were trained to fly through an experimental tunnel consisting of parallel vertical walls. Flights were recorded when (1) the distance between the tunnel walls was either 15 or 30 cm, (2) the visual texture on the tunnel walls provided either strong or weak optic flow cues and (3) the distance between the walls changed abruptly halfway along the tunnel's length. The results reveal that bumblebees regulate ground speed using optic flow cues and that changes in the rate of optic flow are detected at a minimum viewing angle of 23-30 deg., with a visual field that extends to approximately 155 deg. By measuring optic flow over a visual field that has a low minimum viewing angle, bumblebees are able to detect and respond to changes in the proximity of the environment well before they are encountered.

Multi-species sensing using multi-mode absorption spectroscopy with mid-infrared interband cascade lasers

NASA Astrophysics Data System (ADS)

O'Hagan, S.; Northern, J. H.; Gras, B.; Ewart, P.; Kim, C. S.; Kim, M.; Merritt, C. D.; Bewley, W. W.; Canedy, C. L.; Vurgaftman, I.; Meyer, J. R.

2016-06-01

The application of an interband cascade laser, ICL, to multi-mode absorption spectroscopy, MUMAS, in the mid-infrared region is reported. Measurements of individual mode linewidths of the ICL, derived from the pressure dependence of lineshapes in MUMAS signatures of single, isolated, lines in the spectrum of HCl, were found to be in the range 10-80 MHz. Multi-line spectra of methane were recorded using spectrally limited bandwidths, of approximate width 27 cm-1, defined by an interference filter, and consist of approximately 80 modes at spectral locations spanning the 100 cm-1 bandwidth of the ICL output. Calibration of the methane pressures derived from MUMAS data using a capacitance manometer provided measurements with an uncertainty of 1.1 %. Multi-species sensing is demonstrated by the simultaneous detection of methane, acetylene and formaldehyde in a gas mixture. Individual partial pressures of the three gases are derived from best fits of model MUMAS signatures to the data with an experimental error of 10 %. Using an ICL, with an inter-mode interval of ~10 GHz, MUMAS spectra were recorded at pressures in the range 1-10 mbar, and, based on the data, a potential minimum detection limit of the order of 100 ppmv is estimated for MUMAS at atmospheric pressure using an inter-mode interval of 80 GHz.

46 CFR 11.705 - Route familiarization requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... limitations specified in this section, the number of round trips required to qualify an applicant for a... endorsement as first-class pilot shall furnish evidence of having completed a minimum number of round trips... sought. Evidence of having completed a minimum number of round trips while serving as an observer...

46 CFR 11.705 - Route familiarization requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... limitations specified in this section, the number of round trips required to qualify an applicant for a... endorsement as first-class pilot shall furnish evidence of having completed a minimum number of round trips... sought. Evidence of having completed a minimum number of round trips while serving as an observer...

46 CFR 11.705 - Route familiarization requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... limitations specified in this section, the number of round trips required to qualify an applicant for a... endorsement as first-class pilot shall furnish evidence of having completed a minimum number of round trips... sought. Evidence of having completed a minimum number of round trips while serving as an observer...

14 CFR 135.219 - IFR: Destination airport weather minimums.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false IFR: Destination airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.219 IFR: Destination airport weather... latest weather reports or forecasts, or any combination of them, indicate that weather conditions at the...

14 CFR 135.219 - IFR: Destination airport weather minimums.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false IFR: Destination airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.219 IFR: Destination airport weather... latest weather reports or forecasts, or any combination of them, indicate that weather conditions at the...

14 CFR 135.219 - IFR: Destination airport weather minimums.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false IFR: Destination airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.219 IFR: Destination airport weather... latest weather reports or forecasts, or any combination of them, indicate that weather conditions at the...

14 CFR 135.219 - IFR: Destination airport weather minimums.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false IFR: Destination airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.219 IFR: Destination airport weather... latest weather reports or forecasts, or any combination of them, indicate that weather conditions at the...

14 CFR 135.219 - IFR: Destination airport weather minimums.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false IFR: Destination airport weather minimums... VFR/IFR Operating Limitations and Weather Requirements § 135.219 IFR: Destination airport weather... latest weather reports or forecasts, or any combination of them, indicate that weather conditions at the...

40 CFR 146.62 - Minimum criteria for siting.

Code of Federal Regulations, 2014 CFR

2014-07-01

... structural and stratigraphic geology, the hydrogeology, and the seismicity of the region; (2) An analysis of the local geology and hydrogeology of the well site, including, at a minimum, detailed information...) A determination that the geology of the area can be described confidently and that limits of waste...

40 CFR 146.62 - Minimum criteria for siting.

Code of Federal Regulations, 2012 CFR

2012-07-01

... structural and stratigraphic geology, the hydrogeology, and the seismicity of the region; (2) An analysis of the local geology and hydrogeology of the well site, including, at a minimum, detailed information...) A determination that the geology of the area can be described confidently and that limits of waste...

40 CFR 146.62 - Minimum criteria for siting.

Code of Federal Regulations, 2013 CFR

2013-07-01

... structural and stratigraphic geology, the hydrogeology, and the seismicity of the region; (2) An analysis of the local geology and hydrogeology of the well site, including, at a minimum, detailed information...) A determination that the geology of the area can be described confidently and that limits of waste...

14 CFR 135.225 - IFR: Takeoff, approach and landing minimums.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false IFR: Takeoff, approach and landing minimums. 135.225 Section 135.225 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... VFR/IFR Operating Limitations and Weather Requirements § 135.225 IFR: Takeoff, approach and landing...

14 CFR 135.225 - IFR: Takeoff, approach and landing minimums.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false IFR: Takeoff, approach and landing minimums. 135.225 Section 135.225 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... VFR/IFR Operating Limitations and Weather Requirements § 135.225 IFR: Takeoff, approach and landing...

14 CFR 135.225 - IFR: Takeoff, approach and landing minimums.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false IFR: Takeoff, approach and landing minimums. 135.225 Section 135.225 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... VFR/IFR Operating Limitations and Weather Requirements § 135.225 IFR: Takeoff, approach and landing...

2D tilting MEMS micro mirror integrating a piezoresistive sensor position feedback

NASA Astrophysics Data System (ADS)

Lani, S.; Bayat, D.; Despont, M.

2015-02-01

An integrated position sensor for a dual-axis electromagnetic tilting mirror is presented. This tilting mirror is composed of a silicon based mirror directly assembled on a silicon membrane supported by flexible beams. The position sensors are constituted by 4 Wheatstone bridges of piezoresistors which are fabricated by doping locally the flexible beams. A permanent magnet is attached to the membrane and the scanner is mounted above planar coils deposited on a ceramic substrate to achieve electromagnetic actuation. The performances of the piezoresistive sensors are evaluated by measuring the output signal of the piezoresistors as a function of the tilt of the mirror and the temperature. White light interferometry was performed for all measurement to measure the exact tilt angle. The minimum detectable angle with such sensors was 30µrad (around 13bits) in the range of the minimum resolution of the interferometer. The tilt reproducibility was 0.0186%, obtained by measuring the tilt after repeated actuations with a coil current of 50mA during 30 min and the stability over time was 0.05% in 1h without actuation. The maximum measured tilt angle was 6° (mechanical) limited by nonlinearity of the MEMS system.

Statistical detection of patterns in unidimensional distributions by continuous wavelet transforms

NASA Astrophysics Data System (ADS)

Baluev, R. V.

2018-04-01

Objective detection of specific patterns in statistical distributions, like groupings or gaps or abrupt transitions between different subsets, is a task with a rich range of applications in astronomy: Milky Way stellar population analysis, investigations of the exoplanets diversity, Solar System minor bodies statistics, extragalactic studies, etc. We adapt the powerful technique of the wavelet transforms to this generalized task, making a strong emphasis on the assessment of the patterns detection significance. Among other things, our method also involves optimal minimum-noise wavelets and minimum-noise reconstruction of the distribution density function. Based on this development, we construct a self-closed algorithmic pipeline aimed to process statistical samples. It is currently applicable to single-dimensional distributions only, but it is flexible enough to undergo further generalizations and development.

Determination of limonin in grapefruit juice and other citrus juices by high-performance liquid chromatography.

PubMed

Van Beek, T A; Blaakmeer, A

1989-03-03

A method has been developed for the quantitation of the bitter component limonin in grapefruit juice and other citrus juices. The sample clean-up consisted of centrifugation, filtration and a selective, rapid and reproducible purification with a C2 solid-phase extraction column. The limonin concentration was determined by high-performance liquid chromatography on a C18 column with UV detection at 210 nm. A linear response was obtained from 0.0 to 45 ppm limonin. The minimum detectable amount was 2 ng. The minimum concentration which was detected without concentration with good precision was 0.1 ppm. The method was also used for the determination of limonin in different types of oranges, including navel oranges, mandarins, lemons, limes, pomelos and uglis.

Detection of large scale geomagnetic pulsations by MAGDAS-egypt stations during the solar minimum of the solar cycle 24

NASA Astrophysics Data System (ADS)

Fathy, Ibrahim

2016-07-01

This paper presents a statistical study of different types of large-scale geomagnetic pulsation (Pc3, Pc4, Pc5 and Pi2) detected simultaneously by two MAGDAS stations located at Fayum (Geo. Coordinates 29.18 N and 30.50 E) and Aswan (Geo. Coordinates 23.59 N and 32.51 E) in Egypt. The second order butter-worth band-pass filter has been used to filter and analyze the horizontal H-component of the geomagnetic field in one-second data. The data was collected during the solar minimum of the current solar cycle 24. We list the most energetic pulsations detected by the two stations instantaneously, in addition; the average amplitude of the pulsation signals was calculated.

Annual INTEC Groundwater Monitoring Report for Group 5 - Snake River Plain Aquifer (2001)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roddy, Michael Scott

2002-02-01

This report describes the monitoring activities conducted and presents the results of groundwater sampling and water-level measurements from October 2000 to September 2001. Groundwater samples were initially collected from 41 wells from the Idaho Nuclear Technology and Engineering Center and the Central Facilities Area and analyzed for iodine-129, strontium-90, tritium, gross alpha, gross beta, technetium-99, uranium isotopes, plutonium isotopes, neptunium-237, americium-241, gamma spectrometry, and mercury. Samples from 41 wells were collected in April and May 2001. Additional sampling was conducted in August 2001 and included the two CFA production wells, the CFA point of compliance for the production wells, onemore » well that was previously sampled and five additional monitoring wells. Iodine-129 and strontium-90 were the only analytes above their respective maximum contaminant levels. Iodine-129 was detected just above its maximum contaminant level of 1 pCi/L at two of the Central Facilities Area landfill wells. Iodine-129 was detected in the CFA production wells at 0.35±0.083 pCi/L in CFA-1, but was below detectable activity in CFA-2. Strontium-90 was above its maximum contaminant level of 8 pCi/L in several wells near the Idaho Nuclear Technology and Engineering Center but was below its maximum contaminant level in the downgradient wells at the Central Facilities Area landfills. Sr-90 was not detected in the CFA production wells. Gross beta results generally mirrored the results for strontium-90 and technetium-99. Plutonium isotopes and neptunium-237 were not detected. Uranium-233/234 and uranium-238 isotopes were detected in all samples. Concentrations of background and site wells were similar and are within background limits for total uranium determined by the USGS, suggesting that the concentrations are background. Uranium-235/236 was detected in 11 samples, but all the detected concentrations were similar and near the minimum detectable activity. Americium-241 was detected at three locations near the minimum detectable activity of approximately 0.07 pCi/L. The gamma spectrometry results detected cesium-137 in three samples, potassium-40 at eight locations, and radium-226 at one location. Mercury was below its maximum contaminant level of 2 µg/L in all samples. Gamma spectrometry results for the CFA production wells did not detect any analytes. Water-level measurements were taken from wells in the Idaho Nuclear Technology and Engineering Center, Central Facilities Area, and the area south of Central Facilities Area to evaluate groundwater flow directions. Water-level measurements indicated groundwater flow to the south-southwest from the Idaho Nuclear Technology and Engineering Center.« less

Simulating future uncertainty to guide the selection of survey designs for long-term monitoring

USGS Publications Warehouse

Garman, Steven L.; Schweiger, E. William; Manier, Daniel J.; Gitzen, Robert A.; Millspaugh, Joshua J.; Cooper, Andrew B.; Licht, Daniel S.

2012-01-01

A goal of environmental monitoring is to provide sound information on the status and trends of natural resources (Messer et al. 1991, Theobald et al. 2007, Fancy et al. 2009). When monitoring observations are acquired by measuring a subset of the population of interest, probability sampling as part of a well-constructed survey design provides the most reliable and legally defensible approach to achieve this goal (Cochran 1977, Olsen et al. 1999, Schreuder et al. 2004; see Chapters 2, 5, 6, 7). Previous works have described the fundamentals of sample surveys (e.g. Hansen et al. 1953, Kish 1965). Interest in survey designs and monitoring over the past 15 years has led to extensive evaluations and new developments of sample selection methods (Stevens and Olsen 2004), of strategies for allocating sample units in space and time (Urquhart et al. 1993, Overton and Stehman 1996, Urquhart and Kincaid 1999), and of estimation (Lesser and Overton 1994, Overton and Stehman 1995) and variance properties (Larsen et al. 1995, Stevens and Olsen 2003) of survey designs. Carefully planned, “scientific” (Chapter 5) survey designs have become a standard in contemporary monitoring of natural resources. Based on our experience with the long-term monitoring program of the US National Park Service (NPS; Fancy et al. 2009; Chapters 16, 22), operational survey designs tend to be selected using the following procedures. For a monitoring indicator (i.e. variable or response), a minimum detectable trend requirement is specified, based on the minimum level of change that would result in meaningful change (e.g. degradation). A probability of detecting this trend (statistical power) and an acceptable level of uncertainty (Type I error; see Chapter 2) within a specified time frame (e.g. 10 years) are specified to ensure timely detection. Explicit statements of the minimum detectable trend, the time frame for detecting the minimum trend, power, and acceptable probability of Type I error (α) collectively form the quantitative sampling objective.

Identification of SSR and retrotransposon-based molecular markers linked to morphological characters in oily sunfl ower (Helianthus annuus L.) under natural and water-limited states.

PubMed

Ali, Soleimani Gezeljeh; Darvishzadeh, Reza; Ebrahimi, Asa; Bihamta, Mohammad Reza

2018-03-01

Sunflower is an important source of edible oil. Drought is known as an important factor limiting the growth and productivity of field crops in most parts of the world. Agricultural biotechnology mainly aims at developing crops with higher tolerance to the challenging environmental conditions, such as drought. This study examined a number of morphological characters, along with relative water content (RWC) in 100 inbred sunflower lines. A 10 × 10 simple lattice design with two replications was employed to measure the mentioned parameters under natural and water-limited states during two successive years. In molecular trial, 30 simple sequence repeat (SSR) primer pairs, as well as 14 inter-retrotransposon amplified polymorphism (IRAP) and 14 retrotransposon-microsatellite amplified polymorphism (REMAP) primer combinations were used for DNA fingerprinting of the lines. Most of the examined characters had lower average values under water-limited than natural states. Maximum and minimum reductions were observed in the cases of yield and oil percentage, respectively. The broad-sense heritabilities for all the examined characters were 0.20-0.73 and 0.10-0.34 under natural and water-limited states, respectively. In the studied samples, 8.97% of the 435 possible locus pairs of the SSRs represented significant linkage disequilibrium (LD) levels. In the association analysis using SSR markers, 22 and 21 markers were identified (P ≤ 0.05) for the studied characters under natural and water-limited states, respectively. The corresponding values were 50 and 37 using retrotransposon-based molecular markers. Some detected markers were communal between the characters under water-limited and natural states. This was in line with the phenotypic correlations detected between the characters. Communal markers facilitate the simultaneous selection of several characters and can thus improve the efficacy of selection based on markers in the plant-breeding activities.

Binomial Test Method for Determining Probability of Detection Capability for Fracture Critical Applications

NASA Technical Reports Server (NTRS)

Generazio, Edward R.

2011-01-01

The capability of an inspection system is established by applications of various methodologies to determine the probability of detection (POD). One accepted metric of an adequate inspection system is that for a minimum flaw size and all greater flaw sizes, there is 0.90 probability of detection with 95% confidence (90/95 POD). Directed design of experiments for probability of detection (DOEPOD) has been developed to provide an efficient and accurate methodology that yields estimates of POD and confidence bounds for both Hit-Miss or signal amplitude testing, where signal amplitudes are reduced to Hit-Miss by using a signal threshold Directed DOEPOD uses a nonparametric approach for the analysis or inspection data that does require any assumptions about the particular functional form of a POD function. The DOEPOD procedure identifies, for a given sample set whether or not the minimum requirement of 0.90 probability of detection with 95% confidence is demonstrated for a minimum flaw size and for all greater flaw sizes (90/95 POD). The DOEPOD procedures are sequentially executed in order to minimize the number of samples needed to demonstrate that there is a 90/95 POD lower confidence bound at a given flaw size and that the POD is monotonic for flaw sizes exceeding that 90/95 POD flaw size. The conservativeness of the DOEPOD methodology results is discussed. Validated guidelines for binomial estimation of POD for fracture critical inspection are established.

40 CFR Figure C-4 to Subpart C of... - Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10−2.5 Class II...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 6 2012-07-01 2012-07-01 false Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10â2.5 Class II and III Methods C Figure C-4 to Subpart C of Part 53... Methods and Reference Methods Pt. 53, Subpt. C, Fig. C-4 Figure C-4 to Subpart C of Part 53—Illustration...

40 CFR Figure C-4 to Subpart C of... - Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10−2.5 Class II...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 5 2011-07-01 2011-07-01 false Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10â2.5 Class II and III Methods C Figure C-4 to Subpart C of Part 53... Methods and Reference Methods Pt. 53, Subpt. C, Fig. C-4 Figure C-4 to Subpart C of Part 53—Illustration...

40 CFR Figure C-4 to Subpart C of... - Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10−2.5 Class II...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 5 2010-07-01 2010-07-01 false Illustration of the Minimum Limits for Correlation Coefficient for PM2.5 and PM10â2.5 Class II and III Methods C Figure C-4 to Subpart C of Part 53... Methods and Reference Methods Pt. 53, Subpt. C, Fig. C-4 Figure C-4 to Subpart C of Part 53—Illustration...

Robust high-contrast companion detection from interferometric observations. The CANDID algorithm and an application to six binary Cepheids

NASA Astrophysics Data System (ADS)

Gallenne, A.; Mérand, A.; Kervella, P.; Monnier, J. D.; Schaefer, G. H.; Baron, F.; Breitfelder, J.; Le Bouquin, J. B.; Roettenbacher, R. M.; Gieren, W.; Pietrzyński, G.; McAlister, H.; ten Brummelaar, T.; Sturmann, J.; Sturmann, L.; Turner, N.; Ridgway, S.; Kraus, S.

2015-07-01

Context. Long-baseline interferometry is an important technique to spatially resolve binary or multiple systems in close orbits. By combining several telescopes together and spectrally dispersing the light, it is possible to detect faint components around bright stars in a few hours of observations. Aims: We provide a rigorous and detailed method to search for high-contrast companions around stars, determine the detection level, and estimate the dynamic range from interferometric observations. Methods: We developed the code CANDID (Companion Analysis and Non-Detection in Interferometric Data), a set of Python tools that allows us to search systematically for point-source, high-contrast companions and estimate the detection limit using all interferometric observables, i.e., the squared visibilities, closure phases and bispectrum amplitudes. The search procedure is made on a N × N grid of fit, whose minimum needed resolution is estimated a posteriori. It includes a tool to estimate the detection level of the companion in the number of sigmas. The code CANDID also incorporates a robust method to set a 3σ detection limit on the flux ratio, which is based on an analytical injection of a fake companion at each point in the grid. Our injection method also allows us to analytically remove a detected component to 1) search for a second companion; and 2) set an unbiased detection limit. Results: We used CANDID to search for the companions around the binary Cepheids V1334 Cyg, AX Cir, RT Aur, AW Per, SU Cas, and T Vul. First, we showed that our previous discoveries of the components orbiting V1334 Cyg and AX Cir were detected at >25σ and >13σ, respectively. The astrometric positions and flux ratios provided by CANDID for these two stars are in good agreement with our previously published values. The companion around AW Per is detected at more than 15σ with a flux ratio of f = 1.22 ± 0.30%, and it is located at ρ = 32.16 ± 0.29 mas and PA = 67.1 ± 0.3°. We made a possible detection of the companion orbiting RT Aur with f = 0.22 ± 0.11%, and at ρ = 2.10 ± 0.23 mas and PA = -136 ± 6°. It was detected at 3.8σ using the closure phases only, and so more observations are needed to confirm the dectection. No companions were detected around SU Cas and T Vul. We also set the detection limit for possible undetected companions around these stars. We found that there is no companion with a spectral type earlier than B7V, A5V, F0V, B9V, A0V, and B9V orbiting the Cepheids V1334 Cyg, AX Cir, RT Aur, AW Per, SU Cas, and T Vul, respectively. This work also demonstrates the capabilities of the MIRC and PIONIER instruments, which can reach a dynamic range of 1:200, depending on the angular distance of the companion and the (u,v) plane coverage. In the future, we plan to work on improving the sensitivity limits for realistic data through better handling of the correlations. The current version of the code is available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/579/A68

Research on Abnormal Detection Based on Improved Combination of K - means and SVDD

NASA Astrophysics Data System (ADS)

Hao, Xiaohong; Zhang, Xiaofeng

2018-01-01

In order to improve the efficiency of network intrusion detection and reduce the false alarm rate, this paper proposes an anomaly detection algorithm based on improved K-means and SVDD. The algorithm first uses the improved K-means algorithm to cluster the training samples of each class, so that each class is independent and compact in class; Then, according to the training samples, the SVDD algorithm is used to construct the minimum superspheres. The subordinate relationship of the samples is determined by calculating the distance of the minimum superspheres constructed by SVDD. If the test sample is less than the center of the hypersphere, the test sample belongs to this class, otherwise it does not belong to this class, after several comparisons, the final test of the effective detection of the test sample.In this paper, we use KDD CUP99 data set to simulate the proposed anomaly detection algorithm. The results show that the algorithm has high detection rate and low false alarm rate, which is an effective network security protection method.

Automation of an ion chromatograph for precipitation analysis with computerized data reduction

USGS Publications Warehouse

Hedley, Arthur G.; Fishman, Marvin J.

1982-01-01

Interconnection of an ion chromatograph, an autosampler, and a computing integrator to form an analytical system for simultaneous determination of fluoride, chloride, orthophosphate, bromide, nitrate, and sulfate in precipitation samples is described. Computer programs provided with the integrator are modified to implement ionchromatographic data reduction and data storage. The liquid-flow scheme for the ion chromatograph is changed by addition of a second suppressor column for greater analytical capacity. An additional vave enables selection of either suppressor column for analysis, as the other column is regenerated and stabilized with concentrated eluent.Minimum limits of detection and quantitation for each anion are calculated; these limits are a function of suppressor exhaustion. Precision for replicate analyses of six precipitation samples for fluoride, chloride, orthophosphate, nitrate, and sulfate ranged from 0.003 to 0.027 milligrams per liter. To determine accuracy of results, the same samples were spiked with known concentrations of the above mentioned anions. Average recovery was 108 percent.

First Results from the LUX Dark Matter Experiment at the Sanford Underground Research Facility

NASA Astrophysics Data System (ADS)

Akerib, D. S.; Araújo, H. M.; Bai, X.; Bailey, A. J.; Balajthy, J.; Bedikian, S.; Bernard, E.; Bernstein, A.; Bolozdynya, A.; Bradley, A.; Byram, D.; Cahn, S. B.; Carmona-Benitez, M. C.; Chan, C.; Chapman, J. J.; Chiller, A. A.; Chiller, C.; Clark, K.; Coffey, T.; Currie, A.; Curioni, A.; Dazeley, S.; de Viveiros, L.; Dobi, A.; Dobson, J.; Dragowsky, E. M.; Druszkiewicz, E.; Edwards, B.; Faham, C. H.; Fiorucci, S.; Flores, C.; Gaitskell, R. J.; Gehman, V. M.; Ghag, C.; Gibson, K. R.; Gilchriese, M. G. D.; Hall, C.; Hanhardt, M.; Hertel, S. A.; Horn, M.; Huang, D. Q.; Ihm, M.; Jacobsen, R. G.; Kastens, L.; Kazkaz, K.; Knoche, R.; Kyre, S.; Lander, R.; Larsen, N. A.; Lee, C.; Leonard, D. S.; Lesko, K. T.; Lindote, A.; Lopes, M. I.; Lyashenko, A.; Malling, D. C.; Mannino, R.; McKinsey, D. N.; Mei, D.-M.; Mock, J.; Moongweluwan, M.; Morad, J.; Morii, M.; Murphy, A. St. J.; Nehrkorn, C.; Nelson, H.; Neves, F.; Nikkel, J. A.; Ott, R. A.; Pangilinan, M.; Parker, P. D.; Pease, E. K.; Pech, K.; Phelps, P.; Reichhart, L.; Shutt, T.; Silva, C.; Skulski, W.; Sofka, C. J.; Solovov, V. N.; Sorensen, P.; Stiegler, T.; O'Sullivan, K.; Sumner, T. J.; Svoboda, R.; Sweany, M.; Szydagis, M.; Taylor, D.; Tennyson, B.; Tiedt, D. R.; Tripathi, M.; Uvarov, S.; Verbus, J. R.; Walsh, N.; Webb, R.; White, J. T.; White, D.; Witherell, M. S.; Wlasenko, M.; Wolfs, F. L. H.; Woods, M.; Zhang, C.; LUX Collaboration

2014-03-01

The Large Underground Xenon (LUX) experiment is a dual-phase xenon time-projection chamber operating at the Sanford Underground Research Facility (Lead, South Dakota). The LUX cryostat was filled for the first time in the underground laboratory in February 2013. We report results of the first WIMP search data set, taken during the period from April to August 2013, presenting the analysis of 85.3 live days of data with a fiducial volume of 118 kg. A profile-likelihood analysis technique shows our data to be consistent with the background-only hypothesis, allowing 90% confidence limits to be set on spin-independent WIMP-nucleon elastic scattering with a minimum upper limit on the cross section of 7.6×10-46 cm2 at a WIMP mass of 33 GeV/c2. We find that the LUX data are in disagreement with low-mass WIMP signal interpretations of the results from several recent direct detection experiments.

First results from the LUX dark matter experiment at the Sanford underground research facility.

PubMed

Akerib, D S; Araújo, H M; Bai, X; Bailey, A J; Balajthy, J; Bedikian, S; Bernard, E; Bernstein, A; Bolozdynya, A; Bradley, A; Byram, D; Cahn, S B; Carmona-Benitez, M C; Chan, C; Chapman, J J; Chiller, A A; Chiller, C; Clark, K; Coffey, T; Currie, A; Curioni, A; Dazeley, S; de Viveiros, L; Dobi, A; Dobson, J; Dragowsky, E M; Druszkiewicz, E; Edwards, B; Faham, C H; Fiorucci, S; Flores, C; Gaitskell, R J; Gehman, V M; Ghag, C; Gibson, K R; Gilchriese, M G D; Hall, C; Hanhardt, M; Hertel, S A; Horn, M; Huang, D Q; Ihm, M; Jacobsen, R G; Kastens, L; Kazkaz, K; Knoche, R; Kyre, S; Lander, R; Larsen, N A; Lee, C; Leonard, D S; Lesko, K T; Lindote, A; Lopes, M I; Lyashenko, A; Malling, D C; Mannino, R; McKinsey, D N; Mei, D-M; Mock, J; Moongweluwan, M; Morad, J; Morii, M; Murphy, A St J; Nehrkorn, C; Nelson, H; Neves, F; Nikkel, J A; Ott, R A; Pangilinan, M; Parker, P D; Pease, E K; Pech, K; Phelps, P; Reichhart, L; Shutt, T; Silva, C; Skulski, W; Sofka, C J; Solovov, V N; Sorensen, P; Stiegler, T; O'Sullivan, K; Sumner, T J; Svoboda, R; Sweany, M; Szydagis, M; Taylor, D; Tennyson, B; Tiedt, D R; Tripathi, M; Uvarov, S; Verbus, J R; Walsh, N; Webb, R; White, J T; White, D; Witherell, M S; Wlasenko, M; Wolfs, F L H; Woods, M; Zhang, C

2014-03-07

The Large Underground Xenon (LUX) experiment is a dual-phase xenon time-projection chamber operating at the Sanford Underground Research Facility (Lead, South Dakota). The LUX cryostat was filled for the first time in the underground laboratory in February 2013. We report results of the first WIMP search data set, taken during the period from April to August 2013, presenting the analysis of 85.3 live days of data with a fiducial volume of 118 kg. A profile-likelihood analysis technique shows our data to be consistent with the background-only hypothesis, allowing 90% confidence limits to be set on spin-independent WIMP-nucleon elastic scattering with a minimum upper limit on the cross section of 7.6 × 10(-46) cm(2) at a WIMP mass of 33 GeV/c(2). We find that the LUX data are in disagreement with low-mass WIMP signal interpretations of the results from several recent direct detection experiments.

Determination of semicarbazide in fish by molecularly imprinted stir bar sorptive extraction coupled with high performance liquid chromatography.

PubMed

Tang, Tang; Wei, Fangdi; Wang, Xu; Ma, Yujie; Song, Yueyue; Ma, Yunsu; Song, Quan; Xu, Guanhong; Cen, Yao; Hu, Qin

2018-02-15

A novel molecularly imprinted stir bar (MI-SB) for sorptive extraction of semicarbazide (SEM) was prepared in present paper. The coating of the stir bar was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, dynamic adsorption and static adsorption tests. The saturated adsorption of MI-SB was about 4 times over that of non-imprinted stir bar (NI-SB). The selectivity of MI-SB for SEM was much better than NI-SB. A method to determine SEM was established by coupling MI-SB sorptive extraction with HPLC-UV. The liner range was 1-100ng/mL for SEM with a correlation coefficient of 0.9985. The limit of detection was about 0.59ng/mL, which was below the minimum required performance limit of SEM in meat products regulated by European Union. The method was applied to the determination of SEM in fish samples with satisfactory results. Copyright © 2018 Elsevier B.V. All rights reserved.

Isolating contour information from arbitrary images

NASA Technical Reports Server (NTRS)

Jobson, Daniel J.

1989-01-01

Aspects of natural vision (physiological and perceptual) serve as a basis for attempting the development of a general processing scheme for contour extraction. Contour information is assumed to be central to visual recognition skills. While the scheme must be regarded as highly preliminary, initial results do compare favorably with the visual perception of structure. The scheme pays special attention to the construction of a smallest scale circular difference-of-Gaussian (DOG) convolution, calibration of multiscale edge detection thresholds with the visual perception of grayscale boundaries, and contour/texture discrimination methods derived from fundamental assumptions of connectivity and the characteristics of printed text. Contour information is required to fall between a minimum connectivity limit and maximum regional spatial density limit at each scale. Results support the idea that contour information, in images possessing good image quality, is (centered at about 10 cyc/deg and 30 cyc/deg). Further, lower spatial frequency channels appear to play a major role only in contour extraction from images with serious global image defects.

A new hybrid double divisor ratio spectra method for the analysis of ternary mixtures

NASA Astrophysics Data System (ADS)

Youssef, Rasha M.; Maher, Hadir M.

2008-10-01

A new spectrophotometric method was developed for the simultaneous determination of ternary mixtures, without prior separation steps. This method is based on convolution of the double divisor ratio spectra, obtained by dividing the absorption spectrum of the ternary mixture by a standard spectrum of two of the three compounds in the mixture, using combined trigonometric Fourier functions. The magnitude of the Fourier function coefficients, at either maximum or minimum points, is related to the concentration of each drug in the mixture. The mathematical explanation of the procedure is illustrated. The method was applied for the assay of a model mixture consisting of isoniazid (ISN), rifampicin (RIF) and pyrazinamide (PYZ) in synthetic mixtures, commercial tablets and human urine samples. The developed method was compared with the double divisor ratio spectra derivative method (DDRD) and derivative ratio spectra-zero-crossing method (DRSZ). Linearity, validation, accuracy, precision, limits of detection, limits of quantitation, and other aspects of analytical validation are included in the text.

Enhancing the sensitivity of mid-IR quantum cascade laser-based cavity-enhanced absorption spectroscopy using RF current perturbation.

PubMed

Manfred, Katherine M; Kirkbride, James M R; Ciaffoni, Luca; Peverall, Robert; Ritchie, Grant A D

2014-12-15

The sensitivity of mid-IR quantum cascade laser (QCL) off-axis cavity-enhanced absorption spectroscopy (CEAS), often limited by cavity mode structure and diffraction losses, was enhanced by applying a broadband RF noise to the laser current. A pump-probe measurement demonstrated that the addition of bandwidth-limited white noise effectively increased the laser linewidth, thereby reducing mode structure associated with CEAS. The broadband noise source offers a more sensitive, more robust alternative to applying single-frequency noise to the laser. Analysis of CEAS measurements of a CO(2) absorption feature at 1890  cm(-1) averaged over 100 ms yielded a minimum detectable absorption of 5.5×10(-3)  Hz(-1/2) in the presence of broadband RF perturbation, nearly a tenfold improvement over the unperturbed regime. The short acquisition time makes this technique suitable for breath applications requiring breath-by-breath gas concentration information.

Relation between inflammables and ignition sources in aircraft environments

NASA Technical Reports Server (NTRS)

Scull, Wilfred E

1951-01-01

A literature survey was conducted to determine the relation between aircraft ignition sources and inflammables. Available literature applicable to the problem of aircraft fire hazards is analyzed and discussed. Data pertaining to the effect of many variables on ignition temperatures, minimum ignition pressures, minimum spark-ignition energies of inflammables, quenching distances of electrode configurations, and size of openings through which flame will not propagate are presented and discussed. Ignition temperatures and limits of inflammability of gasoline in air in different test environments, and the minimum ignition pressures and minimum size of opening for flame propagation in gasoline-air mixtures are included; inerting of gasoline-air mixtures is discussed.

50 CFR 622.56 - Size limits.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Shrimp Fishery of the Gulf of Mexico § 622.56 Size limits. Shrimp not in compliance with the applicable size limit as... shrimp harvested in the Gulf EEZ are subject to the minimum-size landing and possession limits of...

50 CFR 622.56 - Size limits.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Shrimp Fishery of the Gulf of Mexico § 622.56 Size limits. Shrimp not in compliance with the applicable size limit as... shrimp harvested in the Gulf EEZ are subject to the minimum-size landing and possession limits of...

Advancements in dynamic kill calculations for blowout wells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kouba, G.E.; MacDougall, G.R.; Schumacher, B.W.

1993-09-01

This paper addresses the development, interpretation, and use of dynamic kill equations. To this end, three simple calculation techniques are developed for determining the minimum dynamic kill rate. Two techniques contain only single-phase calculations and are independent of reservoir inflow performance. Despite these limitations, these two methods are useful for bracketing the minimum flow rates necessary to kill a blowing well. For the third technique, a simplified mechanistic multiphase-flow model is used to determine a most-probable minimum kill rate.

Airborne Collision Detection and Avoidance for Small UAS Sense and Avoid Systems

NASA Astrophysics Data System (ADS)

Sahawneh, Laith Rasmi

The increasing demand to integrate unmanned aircraft systems (UAS) into the national airspace is motivated by the rapid growth of the UAS industry, especially small UAS weighing less than 55 pounds. Their use however has been limited by the Federal Aviation Administration regulations due to collision risk they pose, safety and regulatory concerns. Therefore, before civil aviation authorities can approve routine UAS flight operations, UAS must be equipped with sense-and-avoid technology comparable to the see-and-avoid requirements for manned aircraft. The sense-and-avoid problem includes several important aspects including regulatory and system-level requirements, design specifications and performance standards, intruder detecting and tracking, collision risk assessment, and finally path planning and collision avoidance. In this dissertation, our primary focus is on developing an collision detection, risk assessment and avoidance framework that is computationally affordable and suitable to run on-board small UAS. To begin with, we address the minimum sensing range for the sense-and-avoid (SAA) system. We present an approximate close form analytical solution to compute the minimum sensing range to safely avoid an imminent collision. The approach is then demonstrated using a radar sensor prototype that achieves the required minimum sensing range. In the area of collision risk assessment and collision prediction, we present two approaches to estimate the collision risk of an encounter scenario. The first is a deterministic approach similar to those been developed for Traffic Alert and Collision Avoidance (TCAS) in manned aviation. We extend the approach to account for uncertainties of state estimates by deriving an analytic expression to propagate the error variance using Taylor series approximation. To address unanticipated intruders maneuvers, we propose an innovative probabilistic approach to quantify likely intruder trajectories and estimate the probability of collision risk using the uncorrelated encounter model (UEM) developed by MIT Lincoln Laboratory. We evaluate the proposed approach using Monte Carlo simulations and compare the performance with linearly extrapolated collision detection logic. For the path planning and collision avoidance part, we present multiple reactive path planning algorithms. We first propose a collision avoidance algorithm based on a simulated chain that responds to a virtual force field produced by encountering intruders. The key feature of the proposed approach is to model the future motion of both the intruder and the ownship using a chain of waypoints that are equally spaced in time. This timing information is used to continuously re-plan paths that minimize the probability of collision. Second, we present an innovative collision avoidance logic using an ownship centered coordinate system. The technique builds a graph in the local-level frame and uses the Dijkstra's algorithm to find the least cost path. An advantage of this approach is that collision avoidance is inherently a local phenomenon and can be more naturally represented in the local coordinates than the global coordinates. Finally, we propose a two step path planner for ground-based SAA systems. In the first step, an initial suboptimal path is generated using A* search. In the second step, using the A* solution as an initial condition, a chain of unit masses connected by springs and dampers evolves in a simulated force field. The chain is described by a set of ordinary differential equations that is driven by virtual forces to find the steady-state equilibrium. The simulation results show that the proposed approach produces collision-free plans while minimizing the path length. To move towards a deployable system, we apply collision detection and avoidance techniques to a variety of simulation and sensor modalities including camera, radar and ADS-B along with suitable tracking schemes. Keywords: unmanned aircraft system, small UAS, sense and avoid, minimum sensing range, airborne collision detection and avoidance, collision detection, collision risk assessment, collision avoidance, conflict detection, conflict avoidance, path planning.

Minimum depth of investigation for grounded-wire TEM due to self-transients

NASA Astrophysics Data System (ADS)

Zhou, Nannan; Xue, Guoqiang

2018-05-01

The grounded-wire transient electromagnetic method (TEM) has been widely used for near-surface metalliferous prospecting, oil and gas exploration, and hydrogeological surveying in the subsurface. However, it is commonly observed that such TEM signal is contaminated by the self-transient process occurred at the early stage of data acquisition. Correspondingly, there exists a minimum depth of investigation, above which the observed signal is not applicable for reliable data processing and interpretation. Therefore, for achieving a more comprehensive understanding of the TEM method, it is necessary to perform research on the self-transient process and moreover develop an approach for quantifying the minimum detection depth. In this paper, we first analyze the temporal procedure of the equivalent circuit of the TEM method and present a theoretical equation for estimating the self-induction voltage based on the inductor of the transmitting wire. Then, numerical modeling is applied for building the relationship between the minimum depth of investigation and various properties, including resistivity of the earth, offset, and source length. It is guide for the design of survey parameters when the grounded-wire TEM is applied to the shallow detection. Finally, it is verified through applications to a coal field in China.

Sensitive Precise p H Measurement with Large-Area Graphene Field-Effect Transistors at the Quantum-Capacitance Limit

NASA Astrophysics Data System (ADS)

Fakih, Ibrahim; Mahvash, Farzaneh; Siaj, Mohamed; Szkopek, Thomas

2017-10-01

A challenge for p H sensing is decreasing the minimum measurable p H per unit bandwidth in an economical fashion. Minimizing noise to reach the inherent limit imposed by charge fluctuation remains an obstacle. We demonstrate here graphene-based ion-sensing field-effect transistors that saturate the physical limit of sensitivity, defined here as the change in electrical response with respect to p H , and achieve a precision limited by charge-fluctuation noise at the sensing layer. We present a model outlining the necessity for maximizing the device carrier mobility, active sensing area, and capacitive coupling in order to minimize noise. We encapsulate large-area graphene with an ultrathin layer of parylene, a hydrophobic polymer, and deposit an ultrathin, stoichiometric p H -sensing layer of either aluminum oxide or tantalum pentoxide. With these structures, we achieve gate capacitances ˜0.6 μ F /cm2 , approaching the quantum-capacitance limit inherent to graphene, along with a near-Nernstian p H response of ˜55 ±2 mV /p H . We observe field-effect mobilities as high as 7000 cm2 V-1 s-1 with minimal hysteresis as a result of the parylene encapsulation. A detection limit of 0.1 m p H in a 60-Hz electrical bandwidth is observed in optimized graphene transistors.

CW EC-QCL-based sensor for simultaneous detection of H 2O, HDO, N 2O and CH 4 using multi-pass absorption spectroscopy

DOE PAGES

Yu, Yajun; Sanchez, Nancy P.; Griffin, Robert J.; ...

2016-05-03

A sensor system based on a continuous wave, external-cavity quantum-cascade laser (CW EC-QCL) was demonstrated for simultaneous detection of atmospheric H 2O, HDO, N 2O and CH 4 using a compact, dense pattern multi-pass gas cell with an effective path-length of 57.6 m. The EC-QCL with a mode-hop-free spectral range of 1225-1285 cm -1 operating at similar to 7.8 mu m was scanned covering four neighboring absorption lines, for H 2O at 1281.161 cm -1, HDO at 1281.455 cm -1, N 2O at 1281.53 cm -1 and CH 4 at 1281.61 cm -1. A first-harmonic-normalized wavelength modulation spectroscopy with second-harmonicmore » detection (WMS-2f/1f) strategy was employed for data processing. An Allan-Werle deviation analysis indicated that minimum detection limits of 1.77 ppmv for H 2O, 3.92 ppbv for HDO, 1.43 ppbv for N 2O, and 2.2 ppbv for CH 4 were achieved with integration times of 50-s, 50-s, 100-s and 129-s, respectively. In conclusion, experimental measurements of ambient air are also reported.« less

Empirical assessment of the detection efficiency of CR-39 at high proton fluence and a compact, proton detector for high-fluence applications

DOE PAGES

Rosenberg, M. J.; Séguin, F. H.; Waugh, C. J.; ...

2014-04-14

CR-39 solid-state nuclear track detectors are widely used in physics and in many inertial confinement fusion (ICF) experiments, and under ideal conditions these detectors have 100% detection efficiency for ~0.5–8 MeV protons. When the fluence of incident particles becomes too high, the overlap of particle tracks leads to under-counting at typical processing conditions (5h etch in 6N NaOH at 80°C). Short etch times required to avoid overlap can cause under-counting as well, as tracks are not fully developed. Experiments have determined the minimum etch times for 100% detection of 1.7–4.3-MeV protons and established that for 2.4-MeV protons, relevant for detectionmore » of DD protons, the maximum fluence that can be detected using normal processing techniques is ≲3 ×10 6 cm -2. A CR-39-based proton detector has been developed to mitigate issues related to high particle fluences on ICF facilities. Using a pinhole and scattering foil several mm in front of the CR-39, proton fluences at the CR-39 are reduced by more than a factor of ~50, increasing the operating yield upper limit by a comparable amount.« less

Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy

NASA Astrophysics Data System (ADS)

Waterhouse, Dale J.; Joseph, James; Neves, André A.; di Pietro, Massimiliano; Brindle, Kevin M.; Fitzgerald, Rebecca C.; Bohndiek, Sarah E.

2016-08-01

Barrett's esophagus is a known precursor lesion to esophageal adenocarcinoma. In these patients, early detection of premalignant disease, known as dysplasia, allows curative minimally invasive endoscopic therapy, but is confounded by a lack of contrast in white light endoscopy. Imaging fluorescently labeled lectins applied topically to the tissue has the potential to more accurately delineate dysplasia, but tissue autofluorescence limits both sensitivity and contrast when operating in the visible region. To overcome this challenge, we synthesized near-infrared (NIR) fluorescent wheat germ agglutinin (WGA-IR800CW) and constructed a clinically translatable bimodal NIR and white light endoscope. Images of NIR and white light with a field of view of 63 deg and an image resolution of 182 μm are coregistered and the honeycomb artifact arising from the fiber bundle is removed. A minimum detectable concentration of 110 nM was determined using a dilution series of WGA-IR800CW. We demonstrated ex vivo that this system can distinguish between gastric and squamous tissue types in mouse stomachs (p=0.0005) and accurately detect WGA-IR800CW fluorescence in human esophageal resections (compared with a gold standard imaging system, rs>0.90). Based on these findings, future work will optimize the bimodal endoscopic system for clinical trials in Barrett's surveillance.

Quantum cascade laser-based sensor system for nitric oxide detection

NASA Astrophysics Data System (ADS)

Tittel, Frank K.; Allred, James J.; Cao, Yingchun; Sanchez, Nancy P.; Ren, Wei; Jiang, Wenzhe; Jiang, Dongfang; Griffin, Robert J.

2015-01-01

Sensitive detection of nitric oxide (NO) at ppbv concentration levels has an important impact in diverse fields of applications including environmental monitoring, industrial process control and medical diagnostics. For example, NO can be used as a biomarker of asthma and inflammatory lung diseases such as chronic obstructive pulmonary disease. Trace gas sensor systems capable of high sensitivity require the targeting of strong rotational-vibrational bands in the mid-IR spectral range. These bands are accessible using state-of-the-art high heat load (HHL) packaged, continuous wave (CW), distributed feedback (DFB) quantum cascade lasers (QCLs). Quartz-enhanced photoacoustic spectroscopy (QEPAS) permits the design of fast, sensitive, selective, and compact sensor systems. A QEPAS sensor was developed employing a room-temperature CW DFB-QCL emitting at 5.26 μm with an optical excitation power of 60 mW. High sensitivity is achieved by targeting a NO absorption line at 1900.08 cm-1 free of interference by H2O and CO2. The minimum detection limit of the sensor is 7.5 and 1 ppbv of NO with 1and 100 second averaging time respectively . The sensitivity of the sensor system is sufficient for detecting NO in exhaled human breath, with typical concentration levels ranging from 24.0 ppbv to 54.0 ppbv.

DMSO Assisted Electrospray Ionization for the Detection of Small Peptide Hormones in Urine by Dilute-and-Shoot-Liquid-Chromatography-High Resolution Mass Spectrometry

NASA Astrophysics Data System (ADS)

Judák, Péter; Grainger, Janelle; Goebel, Catrin; Van Eenoo, Peter; Deventer, Koen

2017-08-01

The mobile phase additive (DMSO) has been described as a useful tool to enhance electrospray ionization (ESI) of peptides and proteins. So far, this technique has mainly been used in proteomic/peptide research, and its applicability in a routine clinical laboratory setting (i.e., doping control analysis) has not been described yet. This work provides a simple, easy to implement screening method for the detection of doping relevant small peptides (GHRPs, GnRHs, GHS, and vasopressin-analogues) with molecular weight less than 2 kDa applying DMSO in the mobile phase. The gain in sensitivity was sufficient to inject the urine samples after a 2-fold dilution step omitting a time consuming sample preparation. The employed analytical procedure was validated for the qualitative determination of 36 compounds, including 13 metabolites. The detection limits (LODs) ranged between 50 and 1000 pg/mL and were compliant with the 2 ng/mL minimum detection level required by the World Anti-Doping Agency (WADA) for all the target peptides. To demonstrate the feasibility of the work, urine samples obtained from patients who have been treated with desmopressin or leuprolide and urine samples that have been declared as adverse analytical findings were analyzed.

Biological monitoring of 2,4,5-trichlorophenol (I): preparation of antibodies and development of an immunoassay using theoretical models.

PubMed

Nichkova, Mikaela; Galve, Roger; Marco, M-Pilar

2002-11-01

Antibodies against 2,4,5-trichlorophenol have been prepared after theoretical and molecular modeling chemical studies of three potential immunizing haptens with the aim to find out the one mimicking best the target analyte. Competitive direct and indirect ELISAs have been developed after screening a battery of haptenized enzyme tracers and coating antigens, respectively. The relation between the degree of heterology of the competitor and the resulting immunoassay detectability has been investigated according to the electronic similarities of the competitor haptens with the target analyte taking in consideration their pK(a) values. These studies have been performed using theoretical and molecular modeling tools to find out their electronic distribution at their minimum energetic levels. The results suggest that the competitors should have a high homology to produced assays with good detectability values. On the other hand detectability improves when lowering the hapten density of the competitors. An indirect competitive ELISA has been finally selected for further investigation. The immunoassay has an IC(50) value of 0.6 microg L(-)(1) and a limit of detection of 0.084 microg L(-)(1). The selectivity of the assay is high in relation to other chlorophenols frequently present in real samples. In contrast, the brominated analogues may also be recognized with this assay.

Analysis of UAS DAA Surveillance in Fast-Time Simulations without DAA Mitigation

NASA Technical Reports Server (NTRS)

Thipphavong, David P.; Santiago, Confesor; Isaacson, David R.; Lee, Seung Man; Refai, Mohamad Said; Snow, James William

2015-01-01

Realization of the expected proliferation of Unmanned Aircraft System (UAS) operations in the National Airspace System (NAS) depends on the development and validation of performance standards for UAS Detect and Avoid (DAA) Systems. The RTCA Special Committee 228 is charged with leading the development of draft Minimum Operational Performance Standards (MOPS) for UAS DAA Systems. NASA, as a participating member of RTCA SC-228 is committed to supporting the development and validation of draft requirements for DAA surveillance system performance. A recent study conducted using NASA's ACES (Airspace Concept Evaluation System) simulation capability begins to address questions surrounding the development of draft MOPS for DAA surveillance systems. ACES simulations were conducted to study the performance of sensor systems proposed by the SC-228 DAA Surveillance sub-group. Analysis included but was not limited to: 1) number of intruders (both IFR and VFR) detected by all sensors as a function of UAS flight time, 2) number of intruders (both IFR and VFR) detected by radar alone as a function of UAS flight time, and 3) number of VFR intruders detected by all sensors as a function of UAS flight time. The results will be used by SC-228 to inform decisions about the surveillance standards of UAS DAA systems and future requirements development and validation efforts.

Sensitive and rapid detection of Chlamydia trachomatis by recombinase polymerase amplification directly from urine samples.

PubMed

Krõlov, Katrin; Frolova, Jekaterina; Tudoran, Oana; Suhorutsenko, Julia; Lehto, Taavi; Sibul, Hiljar; Mäger, Imre; Laanpere, Made; Tulp, Indrek; Langel, Ülo

2014-01-01

Chlamydia trachomatis is the most common sexually transmitted human pathogen. Infection results in minimal to no symptoms in approximately two-thirds of women and therefore often goes undiagnosed. C. trachomatis infections are a major public health concern because of the potential severe long-term consequences, including an increased risk of ectopic pregnancy, chronic pelvic pain, and infertility. To date, several point-of-care tests have been developed for C. trachomatis diagnostics. Although many of them are fast and specific, they lack the required sensitivity for large-scale application. We describe a rapid and sensitive form of detection directly from urine samples. The assay uses recombinase polymerase amplification and has a minimum detection limit of 5 to 12 pathogens per test. Furthermore, it enables detection within 20 minutes directly from urine samples without DNA purification before the amplification reaction. Initial analysis of the assay from clinical patient samples had a specificity of 100% (95% CI, 92%-100%) and a sensitivity of 83% (95% CI, 51%-97%). The whole procedure is fairly simple and does not require specific machinery, making it potentially applicable in point-of-care settings. Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

Automatic derivation of natural and artificial lineaments from ALS point clouds in floodplains

NASA Astrophysics Data System (ADS)

Mandlburger, G.; Briese, C.

2009-04-01

Water flow is one of the most important driving forces in geomorphology and river systems have ever since formed our landscapes. With increasing urbanisation fertile flood plains were more and more cultivated and the defence of valuable settlement areas by dikes and dams became an important issue. Today, we are dealing with landscapes built up by natural as well as man-made artificial forces. In either case the general shape of the terrain can be portrayed by lineaments representing discontinuities of the terrain slope. Our contribution, therefore, presents an automatic method for delineating natural and artificial structure lines based on randomly distributed point data with high density of more than one point/m2. Preferably, the last echoes of airborne laser scanning (ALS) point clouds are used, since the laser signal is able to penetrate vegetation through small gaps in the foliage. Alternatively, point clouds from (multi) image matching can be employed, but poor ground point coverage in vegetated areas is often the limiting factor. Our approach is divided into three main steps: First, potential 2D start segments are detected by analyzing the surface curvature in the vicinity of each data point, second, the detailed 3D progression of each structure line is modelled patch-wise by intersecting surface pairs (e.g. planar patch pairs) based on the detected start segments and by performing line growing and, finally, post-processing like line cleaning, smoothing and networking is carried out in a last step. For the initial detection of start segments a best fitting two dimensional polynomial surface (quadric) is computed in each data point based on a set of neighbouring points, from which the minimum and maximum curvature is derived. Patches showing high maximum and low minimum curvatures indicate linear discontinuities in the surface slope and serve as start segments for the subsequent 3D modelling. Based on the 2D location and orientation of the start segments, surface patches can be identified as to the left or the right of the structure line. For each patch pair the intersection line is determined by least squares adjustment. The stochastic model considers the planimetric accuracy of the start segments, and the vertical measurement errors in the data points. A robust estimation approach is embedded in the patch adjustment for elimination of off-terrain ALS last echo points. Starting from an initial patch pair, structure line modelling is continued in forward and backward direction as long as certain thresholds (e.g. minimum surface intersection angles) are fulfilled. In the final post-processing step the resulting line set is cleaned by connecting corresponding line parts, by removing short line strings of minor relevance, and by thinning the resulting line set with respect to a certain approximation tolerance in order to reduce the amount of line data. Thus, interactive human verification and editing is limited to a minimum. In a real-world example structure lines were computed for a section of the river Main (ALS, last echoes, 4 points/m2) demonstrating the high potential of the proposed method with respect to accuracy and completeness. Terrestrial control measurements have confirmed the high accuracy expectations both in planimetry (<0.4m) and height (<0.2m).

Development and evaluation of a technique for in vivo monitoring of 60Co in human lungs

NASA Astrophysics Data System (ADS)

de Mello, J. Q.; Lucena, E. A.; Dantas, A. L. A.; Dantas, B. M.

2016-07-01

60Co is a fission product of 235U and represents a risk of internal exposure of workers in nuclear power plants, especially those involved in the maintenance of potentially contaminated parts and equipment. The control of 60Co intake by inhalation can be performed through in vivo monitoring. This work describes the evaluation of a technique through the minimum detectable activity and the corresponding minimum detectable effective doses, based on biokinetic and dosimetric models of 60Co in the human body. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intake.

THREE PLANETS ORBITING WOLF 1061

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wright, D. J.; Wittenmyer, R. A.; Tinney, C. G.

We use archival HARPS spectra to detect three planets orbiting the M3 dwarf Wolf 1061 (GJ 628). We detect a 1.36 M{sub ⊕} minimum-mass planet with an orbital period P = 4.888 days (Wolf 1061b), a 4.25 M{sub ⊕} minimum-mass planet with orbital period P = 17.867 days (Wolf 1061c), and a likely 5.21 M{sub ⊕} minimum-mass planet with orbital period P = 67.274 days (Wolf 1061d). All of the planets are of sufficiently low mass that they may be rocky in nature. The 17.867 day planet falls within the habitable zone for Wolf 1061 and the 67.274 day planetmore » falls just outside the outer boundary of the habitable zone. There are no signs of activity observed in the bisector spans, cross-correlation FWHMs, calcium H and K indices, NaD indices, or Hα indices near the planetary periods. We use custom methods to generate a cross-correlation template tailored to the star. The resulting velocities do not suffer the strong annual variation observed in the HARPS DRS velocities. This differential technique should deliver better exploitation of the archival HARPS data for the detection of planets at extremely low amplitudes.« less

Static magnetic Faraday rotation spectroscopy combined with a differential scheme for OH detection

NASA Astrophysics Data System (ADS)

Zhao, Weixiong; Deng, Lunhua; Qian, Xiaodong; Fang, Bo; Gai, Yanbo; Chen, Weidong; Gao, Xiaoming; Zhang, Weijun

2015-04-01

The hydroxyl (OH) radical plays a critical role in atmospheric chemistry due to its high reactivity with volatile organic compounds (VOCs) and other trace gaseous species. Because of its very short life time and very low concentration in the atmosphere, interference-free high sensitivity in-situ OH monitoring by laser spectroscopy represents a real challenge. Faraday rotation spectroscopy (FRS) relies on the particular magneto-optic effect observed for paramagnetic species, which makes it capable of enhancing the detection sensitivity and mitigation of spectral interferences from diamagnetic species in the atmosphere. When an AC magnetic field is used, the Zeeman splitting of the molecular absorption line (and thus the magnetic circular birefringence) is modulated. This provides an 'internal modulation' of the sample, which permits to suppress the external noise like interference fringes. An alternative FRS detection scheme is to use a static magnetic field (DC-field) associated with laser wavelength modulation to effectively modulate the Zeeman splitting of the absorption lines. In the DC field case, wavelength modulation of the laser frequency can provide excellent performance compared to most of the sensing systems based on direct absorption and wavelength modulation spectroscopy. The dimension of the DC solenoid is not limited by the resonant frequency of the RLC circuit, which makes large dimension solenoid coil achievable and the absorption base length could be further increased. By employing a combination of the environmental photochemical reactor or smog chamber with multipass absorption cell, one can lower the minimum detection limit for high accuracy atmospheric chemistry studies. In this paper, we report on the development of a DC field based FRS in conjunction with a balanced detection scheme for OH radical detection at 2.8 μm and the construction of OH chemistry research platform which combined a large dimension superconducting magnetic coil with the multipass cell and photochemical reactor chamber for real time in-situ measurement of OH radical concentration in the chamber.

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

PubMed Central

2012-01-01

Background Gametocyte carriage is essential for malaria transmission and endemicity of disease; thereby it is a target for malaria control strategies. Malaria-infected individuals may harbour gametocytes below the microscopic detection threshold that can be detected by reverse transcription polymerase chain reaction (RT-PCR) targeting gametocyte-specific mRNA. To date, RT-PCR has mainly been applied to the diagnosis of Plasmodium falciparum gametocytes but very limited for that of Plasmodium vivax. Methods A multiplex-nested RT-PCR targeting Pfs25 and Pvs25 mRNA specific to mature gametocytes of P. falciparum and P. vivax, respectively, was developed. The assay was evaluated using blood samples collected in rainy and dry seasons from febrile patients,in a malaria-endemic area in Thailand. Malaria diagnosis was performed by Giemsa-stained blood smears and 18S rRNA PCR. Results The multiplex-nested RT-PCR detected Pfs25 mRNA in 75 of 86 (87.2%) P. falciparum-infected individuals and Pvs25 mRNA in 82 of 90 (91.1%) P. vivax malaria patients diagnosed by 18S rRNA PCR. Gametocytes were detected in 38 (eight P. falciparum and 30 P. vivax) of 157 microscopy positive samples, implying that a large number of patients harbour sub-microscopic gametocytaemia. No seasonal differences in gametocyte carriage were observed for both malaria species diagnosed by multiplex-nested RT-PCR. With single-nested RT-PCR targeting Pfs25 or Pvs25 mRNA as standard, the multiplex-nested RT-PCR offered sensitivities of 97.4% and 98.9% and specificities of 100% and 98.8% for diagnosing mature gametocytes of P. falciparum and P. vivax, respectively. The minimum detection limit of the multiplex-nested PCR was 10 copies of templates. Conclusions The multiplex-nested RT-PCR developed herein is useful for simultaneous assessment of both P. falciparum and P. vivax gametocyte carriage that is prevalent and generally sympatric in several malaria-endemic areas outside Africa. PMID:22682065

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR.

PubMed

Kuamsab, Napaporn; Putaporntip, Chaturong; Pattanawong, Urassaya; Jongwutiwes, Somchai

2012-06-10

Gametocyte carriage is essential for malaria transmission and endemicity of disease; thereby it is a target for malaria control strategies. Malaria-infected individuals may harbour gametocytes below the microscopic detection threshold that can be detected by reverse transcription polymerase chain reaction (RT-PCR) targeting gametocyte-specific mRNA. To date, RT-PCR has mainly been applied to the diagnosis of Plasmodium falciparum gametocytes but very limited for that of Plasmodium vivax. A multiplex-nested RT-PCR targeting Pfs25 and Pvs25 mRNA specific to mature gametocytes of P. falciparum and P. vivax, respectively, was developed. The assay was evaluated using blood samples collected in rainy and dry seasons from febrile patients,in a malaria-endemic area in Thailand. Malaria diagnosis was performed by Giemsa-stained blood smears and 18S rRNA PCR. The multiplex-nested RT-PCR detected Pfs25 mRNA in 75 of 86 (87.2%) P. falciparum-infected individuals and Pvs25 mRNA in 82 of 90 (91.1%) P. vivax malaria patients diagnosed by 18S rRNA PCR. Gametocytes were detected in 38 (eight P. falciparum and 30 P. vivax) of 157 microscopy positive samples, implying that a large number of patients harbour sub-microscopic gametocytaemia. No seasonal differences in gametocyte carriage were observed for both malaria species diagnosed by multiplex-nested RT-PCR. With single-nested RT-PCR targeting Pfs25 or Pvs25 mRNA as standard, the multiplex-nested RT-PCR offered sensitivities of 97.4% and 98.9% and specificities of 100% and 98.8% for diagnosing mature gametocytes of P. falciparum and P. vivax, respectively. The minimum detection limit of the multiplex-nested PCR was 10 copies of templates. The multiplex-nested RT-PCR developed herein is useful for simultaneous assessment of both P. falciparum and P. vivax gametocyte carriage that is prevalent and generally sympatric in several malaria-endemic areas outside Africa.

77 FR 51939 - Temporary Rule To Establish Management Measures for the Limited Harvest and Possession of South...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-28

... decrease regulatory discards (fish returned to the water because they are below the minimum size limit... Atlantic off Florida, and vermilion snapper. (See Sec. 622.32(c)(2) for limitations on cubera snapper...

Minimum Competency Testing: Guidelines for Policymakers and Citizens.

ERIC Educational Resources Information Center

Gray, Dennis

Intended to help the laity find their way through the confusion and controversy surrounding minimum competency testing (MCT), this paper explains what MCT is, acknowledges its limitations, weighs the criticisms, and shows how MCT may be used constructively for the benefit of students and for the improvement of schools. Highlights from the paper…

29 CFR 570.119 - Fourteen-year minimum.

Code of Federal Regulations, 2011 CFR

2011-07-01

... orders lowering the age minimum to 14 years where he or she finds that such employment is confined to... their health and well-being. Pursuant to this authority, the Secretary has detailed in § 570.34 all... school hours and is confined to other specified limits. The Secretary, in order to provide clarity and...

29 CFR 779.507 - Fourteen-year minimum.

Code of Federal Regulations, 2011 CFR

2011-07-01

... the age minimum to 14 years where he finds that such employment is confined to periods which will not... in a limited number of occupations where the work is performed outside school hours and is confined... through 570.38 of this chapter), employment of minors in this age group is not permitted in the following...

29 CFR 779.507 - Fourteen-year minimum.

Code of Federal Regulations, 2012 CFR

2012-07-01

... the age minimum to 14 years where he finds that such employment is confined to periods which will not... in a limited number of occupations where the work is performed outside school hours and is confined... through 570.38 of this chapter), employment of minors in this age group is not permitted in the following...

29 CFR 779.507 - Fourteen-year minimum.

Code of Federal Regulations, 2014 CFR

2014-07-01

... the age minimum to 14 years where he finds that such employment is confined to periods which will not... in a limited number of occupations where the work is performed outside school hours and is confined... through 570.38 of this chapter), employment of minors in this age group is not permitted in the following...

29 CFR 570.119 - Fourteen-year minimum.

Code of Federal Regulations, 2012 CFR

2012-07-01

... orders lowering the age minimum to 14 years where he or she finds that such employment is confined to... their health and well-being. Pursuant to this authority, the Secretary has detailed in § 570.34 all... school hours and is confined to other specified limits. The Secretary, in order to provide clarity and...

29 CFR 570.119 - Fourteen-year minimum.

Code of Federal Regulations, 2013 CFR

2013-07-01

... orders lowering the age minimum to 14 years where he or she finds that such employment is confined to... their health and well-being. Pursuant to this authority, the Secretary has detailed in § 570.34 all... school hours and is confined to other specified limits. The Secretary, in order to provide clarity and...

29 CFR 779.507 - Fourteen-year minimum.

Code of Federal Regulations, 2013 CFR

2013-07-01

... the age minimum to 14 years where he finds that such employment is confined to periods which will not... in a limited number of occupations where the work is performed outside school hours and is confined... through 570.38 of this chapter), employment of minors in this age group is not permitted in the following...

29 CFR 570.119 - Fourteen-year minimum.

Code of Federal Regulations, 2014 CFR

2014-07-01

... orders lowering the age minimum to 14 years where he or she finds that such employment is confined to... their health and well-being. Pursuant to this authority, the Secretary has detailed in § 570.34 all... school hours and is confined to other specified limits. The Secretary, in order to provide clarity and...

49 CFR 230.112 - Wheels and tires.

Code of Federal Regulations, 2010 CFR

2010-10-01

... wheels mounted on the same axle shall not vary more than 1/4 inch. (d) Tire thickness. Wheels may not have tires with a minimum thickness less than that indicated in the table in this paragraph (d). When... the minimum thickness of tires may be as much below the limits specified earlier in this paragraph (d...

Functionalized graphene oxide quantum dot-PVA hydrogel: a colorimetric sensor for Fe2+, Co2+ and Cu2+ ions

NASA Astrophysics Data System (ADS)

Baruah, Upama; Chowdhury, Devasish

2016-04-01

Functionalized graphene oxide quantum dots (GOQDs)-poly(vinyl alcohol) (PVA) hybrid hydrogels were prepared using a simple, facile and cost-effective strategy. GOQDs bearing different surface functional groups were introduced as the cross-linking agent into the PVA matrix thereby resulting in gelation. The four different types of hybrid hydrogels were prepared using graphene oxide, reduced graphene oxide, ester functionalized graphene oxide and amine functionalized GOQDs as cross-linking agents. It was observed that the hybrid hydrogel prepared with amine functionalized GOQDs was the most stable. The potential applicability of using this solid sensing platform has been subsequently explored in an easy, simple, effective and sensitive method for optical detection of M2+ (Fe2+, Co2+ and Cu2+) in aqueous media involving colorimetric detection. Amine functionalized GOQDs-PVA hybrid hydrogel when put into the corresponding solution of Fe2+, Co2+ and Cu2+ renders brown, orange and blue coloration respectively of the solution detecting the presence of Fe2+, Co2+ and Cu2+ ions in the solution. The minimum detection limit observed was 1 × 10-7 M using UV-visible spectroscopy. Further, the applicability of the sensing material was also tested for a mixture of co-existing ions in solution to demonstrate the practical applicability of the system. Insight into the probable mechanistic pathway involved in the detection process is also being discussed.

A new visible-light-excitable ICT-CHEF-mediated fluorescence 'turn-on' probe for the selective detection of Cd(2+) in a mixed aqueous system with live-cell imaging.

PubMed

Goswami, Shyamaprosad; Aich, Krishnendu; Das, Sangita; Das Mukhopadhyay, Chitrangada; Sarkar, Deblina; Mondal, Tapan Kumar

2015-03-28

A new quinoline based sensor was developed and applied for the selective detection of Cd(2+) both in vitro and in vivo. The designed probe displays a straightforward approach for the selective detection of Cd(2+) with a prominent fluorescence enhancement along with a large red shift (∼38 nm), which may be because of the CHEF (chelation-enhanced fluorescence) and ICT (internal charge transfer) processes after interaction with Cd(2+). The interference from other biologically important competing metal ions, particularly Zn(2+), has not been observed. The visible-light excitability of the probe merits in the viewpoint of its biological application. The probe enables the detection of intracellular Cd(2+) with non-cytotoxic effects, which was demonstrated with the live RAW cells. The experimentally observed change in the structure and electronic properties of the sensor after the addition of Cd(2+) were modelled by the density functional theory (DFT) and time-dependent density functional theory (TDDFT) computational calculations, respectively. Moreover, the test strip experiment with this sensor exhibits both absorption and fluorescence color changes when exposed to Cd(2+) in a mixed aqueous solution, which also makes the probe more useful. The minimum limit of detection of Cd(2+) by the probe was in the range of 9.9 × 10(-8) M level.

Critical considerations for the application of environmental DNA methods to detect aquatic species

USGS Publications Warehouse

Goldberg, Caren S.; Turner, Cameron R.; Deiner, Kristy; Klymus, Katy E.; Thomsen, Philip Francis; Murphy, Melanie A.; Spear, Stephen F.; McKee, Anna; Oyler-McCance, Sara J.; Cornman, Robert S.; Laramie, Matthew B.; Mahon, Andrew R.; Lance, Richard F.; Pilliod, David S.; Strickler, Katherine M.; Waits, Lisette P.; Fremier, Alexander K.; Takahara, Teruhiko; Herder, Jelger E.; Taberlet, Pierre

2016-01-01

Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods, however, requires a heightened awareness and attention to quality assurance and quality control protocols. Additionally, the interpretation of eDNA data demands careful consideration of multiple factors. As eDNA methods have grown in application, diverse approaches have been implemented to address these issues. With interest in eDNA continuing to expand, supportive guidelines for undertaking eDNA studies are greatly needed.Environmental DNA researchers from around the world have collaborated to produce this set of guidelines and considerations for implementing eDNA methods to detect aquatic macroorganisms.Critical considerations for study design include preventing contamination in the field and the laboratory, choosing appropriate sample analysis methods, validating assays, testing for sample inhibition and following minimum reporting guidelines. Critical considerations for inference include temporal and spatial processes, limits of correlation of eDNA with abundance, uncertainty of positive and negative results, and potential sources of allochthonous DNA.We present a synthesis of knowledge at this stage for application of this new and powerful detection method.

Simultaneous detection of molecular oxygen and water vapor in the tissue optical window using tunable diode laser spectroscopy.

PubMed

Persson, Linda; Lewander, Märta; Andersson, Mats; Svanberg, Katarina; Svanberg, Sune

2008-04-20

We report on a dual-diode laser spectroscopic system for simultaneous detection of two gases. The technique is demonstrated by performing gas measurements on absorbing samples such as an air distance, and on absorbing and scattering porous samples such as human tissue. In the latter it is possible to derive the concentration of one gas by normalizing to a second gas of known concentration. This is possible if the scattering and absorption of the bulk material is equal or similar for the two wavelengths used, resulting in a common effective pathlength. Two pigtailed diode lasers are operated in a wavelength modulation scheme to detect molecular oxygen ~760 nm and water vapor ~935 nm within the tissue optical window (600 nm to 1.3 mum). Different modulation frequencies are used to distinguish between the two wavelengths. No crosstalk can be observed between the gas contents measured in the two gas channels. The system is made compact by using a computer board and performing software-based lock-in detection. The noise floor obtained corresponds to an absorption fraction of approximately 6x10(-5) for both oxygen and water vapor, yielding a minimum detection limit of ~2 mm for both gases in ambient air. The power of the technique is illustrated by the preliminary results of a clinical trial, nonintrusively investigating gas in human sinuses.

Monitoring Termite-Mediated Ecosystem Processes Using Moderate and High Resolution Satellite Imagery

NASA Astrophysics Data System (ADS)

Lind, B. M.; Hanan, N. P.

2016-12-01

Termites are considered dominant decomposers and prominent ecosystem engineers in the global tropics and they build some of the largest and architecturally most complex non-human-made structures in the world. Termite mounds significantly alter soil texture, structure, and nutrients, and have major implications for local hydrological dynamics, vegetation characteristics, and biological diversity. An understanding of how these processes change across large scales has been limited by our ability to detect termite mounds at high spatial resolutions. Our research develops methods to detect large termite mounds in savannas across extensive geographic areas using moderate and high resolution satellite imagery. We also investigate the effect of termite mounds on vegetation productivity using Landsat-8 maximum composite NDVI data as a proxy for production. Large termite mounds in arid and semi-arid Senegal generate highly reflective `mound scars' with diameters ranging from 10 m at minimum to greater than 30 m. As Sentinel-2 has several bands with 10 m resolution and Landsat-8 has improved calibration, higher radiometric resolution, 15 m spatial resolution (pansharpened), and improved contrast between vegetated and bare surfaces compared to previous Landsat missions, we found that the largest and most influential mounds in the landscape can be detected. Because mounds as small as 4 m in diameter are easily detected in high resolution imagery we used these data to validate detection results and quantify omission errors for smaller mounds.