Surfactant effects on contact line alteration of a liquid drop in a capillary tube

NASA Astrophysics Data System (ADS)

Yulianti, K.; Marwati, R.

2018-05-01

In this paper, the effect of an insoluble surfactant on the moving contact line of an interface between two fluids filling a capillary tube is studied. The governing equations are the incompressible Navier-Stokes equations with the couple of Eulerian fluid variables and Lagrangian interfacial markers. In our model, capillary force plays a role in the fluids motion. Here, we propose that besides lowering the interfacial tension which affects the capillary force, the surfactant also decreases the surface tension between fluids and a solid surface. That condition is applied to the unbalanced Young condition at the contact line. The front-tracking method is used to solve numerically the free boundary motion of the interface. Results show that the surfactant has a significant effect on the motion of the contact line.

Filling of single-walled carbon nanotubes by CuI nanocrystals via capillary technique

NASA Astrophysics Data System (ADS)

Chernysheva, M. V.; Eliseev, A. A.; Lukashin, A. V.; Tretyakov, Yu. D.; Savilov, S. V.; Kiselev, N. A.; Zhigalina, O. M.; Kumskov, A. S.; Krestinin, A. V.; Hutchison, J. L.

2007-03-01

The present study is focused on the synthesis and investigation of the nanocomposite CuI@SWNT obtained by the filling of metallic single-walled carbon nanotubes (SWNTs) (inner diameter 1-1.4 nm) by wide-gap semiconducting CuI nanocrystals using so-called capillary technique. The method is based on the impregnation of pre-opened SWNTs by molten CuI in vacuum with subsequent slow cooling to room temperature. SWNTs and CuI@SWNT nanocomposites were studied by nitrogen capillary adsorption method, EDX microanalysis, HRTEM microscopy and Raman spectroscopy. The changing of electronic properties of CuI@SWNT as compare to row nanotubes was observed.

Modeling of Transient Nectar Flow in Hummingbird Tongues

NASA Astrophysics Data System (ADS)

Rico-Guevara, Alejandro; Fan, Tai-Hsi; Rubega, Margaret

2015-11-01

We demonstrate that hummingbirds do not pick up floral nectar via capillary action. The long believed capillary rise models were mistaken and unable to predict the dynamic nectar intake process. Instead, hummingbird's tongue acts as an elastic micropump. Nectar is drawn into the tongue grooves during elastic expansion after the grooves are squeezed flat by the beak. The new model is compared with experimental data from high-speed videos of 18 species and tens of individuals of wild hummingbirds. Self-similarity and transitions of short-to-long time behaviours have been resolved for the nectar flow driven by expansive filling. The transient dynamics is characterized by the relative contributions of negative excess pressure and the apparent area modulus of the tongue grooves.

Effect of wave action on near-well zone cleaning

NASA Astrophysics Data System (ADS)

Pen'kovskii, V. I.; Korsakova, N. K.

2017-10-01

Drilling filtrate invasion into the producing formation and native water accumulating of the near-well zone in well operation reduce the well productivity. As a result of that, depending on characteristic capillary pressure scale and differential pressure drawdown, oil production rate may become lower than expected one. In this paper, it is considered the hysteresis effects of capillary pressure after reversion of displacement. As applied to laboratory experiment conditions, the solution of problem of oil flow in formation model with a pressure drop on the model sides harmonically varied with time is presented. It was estimated a range of fluid vibration effective action on the near-well zone cleaning from capillary locking water. The plant simulating extraction of oil from formation using widely practised sucker-rod pump has been created. Formation model is presented as a slot filled with broken glass between two plates. In the process, natural oil and sodium chloride solution were used as working fluids. The experiments qualitatively confirm a positive effect of jack pumps on the near-well zone cleaning.

Wettability and Flow Rate Impacts on Immiscible Displacement: A Theoretical Model

NASA Astrophysics Data System (ADS)

Hu, Ran; Wan, Jiamin; Yang, Zhibing; Chen, Yi-Feng; Tokunaga, Tetsu

2018-04-01

When a more viscous fluid displaces a less viscous one in porous media, viscous pressure drop stabilizes the displacement front against capillary pressure fluctuation. For this favorable viscous ratio conditions, previous studies focused on the front instability under slow flow conditions but did not address competing effects of wettability and flow rate. Here we study how this competition controls displacement patterns. We propose a theoretical model that describes the crossover from fingering to stable flow as a function of invading fluid contact angle θ and capillary number Ca. The phase diagram predicted by the model shows that decreasing θ stabilizes the displacement for θ≥45° and the critical contact angle θc increases with Ca. The boundary between corner flow and cooperative filling for θ < 45° is also described. This work extends the classic phase diagram and has potential applications in predicting CO2 capillary trapping and manipulating wettability to enhance gas/oil displacement efficiency.

Dynamic Effective Mass of Granular Media

NASA Astrophysics Data System (ADS)

Hsu, Chaur-Jian; Johnson, David L.; Ingale, Rohit A.; Valenza, John J.; Gland, Nicolas; Makse, Hernán A.

2009-02-01

We develop the concept of frequency dependent effective mass, Mtilde (ω), of jammed granular materials which occupy a rigid cavity to a filling fraction of 48%, the remaining volume being air of normal room condition or controlled humidity. The dominant features of Mtilde (ω) provide signatures of the dissipation of acoustic modes, elasticity, and aging effects in the granular medium. We perform humidity controlled experiments and interpret the data in terms of a continuum model and a “trap” model of thermally activated capillary bridges at the contact points. The results suggest that attenuation of acoustic waves in granular materials can be influenced significantly by the kinetics of capillary condensation between the asperities at the contacts.

Investigation of the Dynamic Contact Angle Using a Direct Numerical Simulation Method.

PubMed

Zhu, Guangpu; Yao, Jun; Zhang, Lei; Sun, Hai; Li, Aifen; Shams, Bilal

2016-11-15

A large amount of residual oil, which exists as isolated oil slugs, remains trapped in reservoirs after water flooding. Numerous numerical studies are performed to investigate the fundamental flow mechanism of oil slugs to improve flooding efficiency. Dynamic contact angle models are usually introduced to simulate an accurate contact angle and meniscus displacement of oil slugs under a high capillary number. Nevertheless, in the oil slug flow simulation process, it is unnecessary to introduce the dynamic contact angle model because of a negligible change in the meniscus displacement after using the dynamic contact angle model when the capillary number is small. Therefore, a critical capillary number should be introduced to judge whether the dynamic contact model should be incorporated into simulations. In this study, a direct numerical simulation method is employed to simulate the oil slug flow in a capillary tube at the pore scale. The position of the interface between water and the oil slug is determined using the phase-field method. The capacity and accuracy of the model are validated using a classical benchmark: a dynamic capillary filling process. Then, different dynamic contact angle models and the factors that affect the dynamic contact angle are analyzed. The meniscus displacements of oil slugs with a dynamic contact angle and a static contact angle (SCA) are obtained during simulations, and the relative error between them is calculated automatically. The relative error limit has been defined to be 5%, beyond which the dynamic contact angle model needs to be incorporated into the simulation to approach the realistic displacement. Thus, the desired critical capillary number can be determined. A three-dimensional universal chart of critical capillary number, which functions as static contact angle and viscosity ratio, is given to provide a guideline for oil slug simulation. Also, a fitting formula is presented for ease of use.

Nanoscale Capillary Flows in Alumina: Testing the Limits of Classical Theory.

PubMed

Lei, Wenwen; McKenzie, David R

2016-07-21

Anodic aluminum oxide (AAO) membranes have well-formed cylindrical channels, as small as 10 nm in diameter, in a close packed hexagonal array. The channels in AAO membranes simulate very small leaks that may be present for example in an aluminum oxide device encapsulation. The 10 nm alumina channel is the smallest that has been studied to date for its moisture flow properties and provides a stringent test of classical capillary theory. We measure the rate at which moisture penetrates channels with diameters in the range of 10 to 120 nm with moist air present at 1 atm on one side and dry air at the same total pressure on the other. We extend classical theory for water leak rates at high humidities by allowing for variable meniscus curvature at the entrance and show that the extended theory explains why the flow increases greatly when capillary filling occurs and enables the contact angle to be determined. At low humidities our measurements for air-filled channels agree well with theory for the interdiffusive flow of water vapor in air. The flow rate of water-filled channels is one order of magnitude less than expected from classical capillary filling theory and is coincidentally equal to the helium flow rate, validating the use of helium leak testing for evaluating moisture flows in aluminum oxide leaks.

Influence of granulometry in the Hurst exponent of air liquid interfaces formed during capillary rising in a granular media

NASA Astrophysics Data System (ADS)

Gontijo, Guilherme L.; Souza, Flávia B.; Braga, Rafael M. L.; Silva, Pedro H. E.; Correia, Maury D.; Atman, A. P. F.

2017-06-01

We report results concerning the fractal dimension of a air/fluid interface formed during the capillary rising of a fluid into a dense granular media. The system consists in a modified Hele-Shaw cell filled with grains at different granulometries and confined in a narrow gap between the glass plates. The system is then placed onto a water reservoir, and the liquid penetrates the medium due to capillary forces. We measure the Hurst exponent of the liquid/air interface with help of image processing, and follow the temporal evolution of the profiles. We observe that the Hurst exponent can be related with the granulometry, but the range of values are odd to the predicted values from models or theory.

Properties of pendular liquid bridges determined on Delaunay's roulettes

NASA Astrophysics Data System (ADS)

Mielniczuk, Boleslaw; Millet, Olivier; Gagneux, Gérard; El Youssoufi, Moulay Said

2017-06-01

This work addresses the study of capillary bridge properties between two grains, with use of recent analytical model, based on solutions of Young-Laplace equation from an inverse problem. A simple explicit criterion allows to classify the profile of capillary bridge as a surface of revolution with constant mean curvature (Delaunay roulette) using its measured geometrical parameters (gorge radius, contact angle, half-filling angle). Necessary data are obtained from experimental tests, realized on liquid bridges between two equal spherical grains. Sequences of images are recorded at several (fixed) volumes of liquid and different separations distances between the spheres (from contact to rupture), in laboratory and in micro-gravity conditions. For each configuration, an exact parametric representation of the meridian is revealed. Mean bridge curvature, internal pressure and intergranular capillary force are also determined.

Early regimes of water capillary flow in slit silica nanochannels.

PubMed

Oyarzua, Elton; Walther, Jens H; Mejía, Andrés; Zambrano, Harvey A

2015-06-14

Molecular dynamics simulations are conducted to investigate the initial stages of spontaneous imbibition of water in slit silica nanochannels surrounded by air. An analysis is performed for the effects of nanoscopic confinement, initial conditions of liquid uptake and air pressurization on the dynamics of capillary filling. The results indicate that the nanoscale imbibition process is divided into three main flow regimes: an initial regime where the capillary force is balanced only by the inertial drag and characterized by a constant velocity and a plug flow profile. In this regime, the meniscus formation process plays a central role in the imbibition rate. Thereafter, a transitional regime takes place, in which, the force balance has significant contributions from both inertia and viscous friction. Subsequently, a regime wherein viscous forces dominate the capillary force balance is attained. Flow velocity profiles identify the passage from an inviscid flow to a developing Poiseuille flow. Gas density profiles ahead of the capillary front indicate a transient accumulation of air on the advancing meniscus. Furthermore, slower capillary filling rates computed for higher air pressures reveal a significant retarding effect of the gas displaced by the advancing meniscus.

Electrically modulated capillary filling imbibition of nematic liquid crystals

NASA Astrophysics Data System (ADS)

Dhar, Jayabrata; Chakraborty, Suman

2018-04-01

The flow of nematic liquid crystals (NLCs) in the presence of an electric field is typically characterized by the variation in its rheological properties due to transition in its molecular arrangements. Here, we bring out a nontrivial interplay of a consequent alteration in the resistive viscous effects and driving electrocapillary interactions, toward maneuvering the capillary filling dynamics over miniaturized scales. Considering a dynamic interplay of the relevant bulk and interfacial forces acting in tandem, our results converge nicely to previously reported experimental data. Finally, we attempt a scaling analysis to bring forth further insight to the reported observations. Our analysis paves the way for the development of microfluidic strategies with previously unexplored paradigms of interaction between electrical and fluidic phenomenon, providing with an augmented controllability on capillary filling as compared to tthose reported to be achievable by the existing strategies. This, in turn, holds utilitarian scopes in improved designs of functional capillarities in electro-optical systems, electrorheological utilities, electrokinetic flow control, as well as in interfacing and imaging systems for biomedical microdevices.

Condensation in Nanoporous Packed Beds.

PubMed

Ally, Javed; Molla, Shahnawaz; Mostowfi, Farshid

2016-05-10

In materials with tiny, nanometer-scale pores, liquid condensation is shifted from the bulk saturation pressure observed at larger scales. This effect is called capillary condensation and can block pores, which has major consequences in hydrocarbon production, as well as in fuel cells, catalysis, and powder adhesion. In this study, high pressure nanofluidic condensation studies are performed using propane and carbon dioxide in a colloidal crystal packed bed. Direct visualization allows the extent of condensation to be observed, as well as inference of the pore geometry from Bragg diffraction. We show experimentally that capillary condensation depends on pore geometry and wettability because these factors determine the shape of the menisci that coalesce when pore filling occurs, contrary to the typical assumption that all pore structures can be modeled as cylindrical and perfectly wetting. We also observe capillary condensation at higher pressures than has been done previously, which is important because many applications involving this phenomenon occur well above atmospheric pressure, and there is little, if any, experimental validation of capillary condensation at such pressures, particularly with direct visualization.

Demonstration of a high repetition rate capillary discharge waveguide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gonsalves, A. J., E-mail: ajgonsalves@lbl.gov; Pieronek, C.; Daniels, J.

2016-01-21

A hydrogen-filled capillary discharge waveguide operating at kHz repetition rates is presented for parameters relevant to laser plasma acceleration (LPA). The discharge current pulse was optimized for erosion mitigation with laser guiding experiments and MHD simulation. Heat flow simulations and measurements showed modest temperature rise at the capillary wall due to the average heat load at kHz repetition rates with water-cooled capillaries, which is promising for applications of LPAs such as high average power radiation sources.

Synthesis of capillary pressure curves from post-stack seismic data with the use of intelligent estimators: A case study from the Iranian part of the South Pars gas field, Persian Gulf Basin

NASA Astrophysics Data System (ADS)

Golsanami, Naser; Kadkhodaie-Ilkhchi, Ali; Erfani, Amir

2015-01-01

Capillary pressure curves are important data for reservoir rock typing, analyzing pore throat distribution, determining height above free water level, and reservoir simulation. Laboratory experiments provide accurate data, however they are expensive, time-consuming and discontinuous through the reservoir intervals. The current study focuses on synthesizing artificial capillary pressure (Pc) curves from seismic attributes with the use of artificial intelligent systems including Artificial Neural Networks (ANNs), Fuzzy logic (FL) and Adaptive Neuro-Fuzzy Inference Systems (ANFISs). The synthetic capillary pressure curves were achieved by estimating pressure values at six mercury saturation points. These points correspond to mercury filled pore volumes of core samples (Hg-saturation) at 5%, 20%, 35%, 65%, 80%, and 90% saturations. To predict the synthetic Pc curve at each saturation point, various FL, ANFIS and ANN models were constructed. The varying neural network models differ in their training algorithm. Based on the performance function, the most accurately functioning models were selected as the final solvers to do the prediction process at each of the above-mentioned mercury saturation points. The constructed models were then tested at six depth points of the studied well which were already unforeseen by the models. The results show that the Fuzzy logic and neuro-fuzzy models were not capable of making reliable estimations, while the predictions from the ANN models were satisfyingly trustworthy. The obtained results showed a good agreement between the laboratory derived and synthetic capillary pressure curves. Finally, a 3D seismic cube was captured for which the required attributes were extracted and the capillary pressure cube was estimated by using the developed models. In the next step, the synthesized Pc cube was compared with the seismic cube and an acceptable correspondence was observed.

Particle sizer and DNA sequencer

DOEpatents

Olivares, Jose A.; Stark, Peter C.

2005-09-13

An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

Effective slip over partially filled microcavities and its possible failure

NASA Astrophysics Data System (ADS)

Ge, Zhouyang; Holmgren, Hanna; Kronbichler, Martin; Brandt, Luca; Kreiss, Gunilla

2018-05-01

Motivated by the emerging applications of liquid-infused surfaces (LIS), we study the drag reduction and robustness of transverse flows over two-dimensional microcavities partially filled with an oily lubricant. Using separate simulations at different scales, characteristic contact line velocities at the fluid-solid intersection are first extracted from nanoscale phase field simulations and then applied to micronscale two-phase flows, thus introducing a multiscale numerical framework to model the interface displacement and deformation within the cavities. As we explore the various effects of the lubricant-to-outer-fluid viscosity ratio μ˜2/μ˜1 , the capillary number Ca, the static contact angle θs, and the filling fraction of the cavity δ , we find that the effective slip is most sensitive to the parameter δ . The effects of μ˜2/μ˜1 and θs are generally intertwined but weakened if δ <1 . Moreover, for an initial filling fraction δ =0.94 , our results show that the effective slip is nearly independent of the capillary number when it is small. Further increasing Ca to about 0.01 μ˜1/μ˜2 , we identify a possible failure mode, associated with lubricants draining from the LIS, for μ˜2/μ˜1≲0.1 . Very viscous lubricants (e.g., μ˜2/μ˜1>1 ), however, are immune to such failure due to their generally larger contact line velocity.

Transmission in situ and operando high temperature X-ray powder diffraction in variable gaseous environments

NASA Astrophysics Data System (ADS)

Schlicker, Lukas; Doran, Andrew; Schneppmüller, Peter; Gili, Albert; Czasny, Mathias; Penner, Simon; Gurlo, Aleksander

2018-03-01

This work describes a device for time-resolved synchrotron-based in situ and operando X-ray powder diffraction measurements at elevated temperatures under controllable gaseous environments. The respective gaseous sample environment is realized via a gas-tight capillary-in-capillary design, where the gas flow is achieved through an open-end 0.5 mm capillary located inside a 0.7 mm capillary filled with a sample powder. Thermal mass flow controllers provide appropriate gas flows and computer-controlled on-the-fly gas mixing capabilities. The capillary system is centered inside an infrared heated, proportional integral differential-controlled capillary furnace allowing access to temperatures up to 1000 °C.

A novel model for simulating the racing effect in capillary-driven underfill process in flip chip

NASA Astrophysics Data System (ADS)

Zhu, Wenhui; Wang, Kanglun; Wang, Yan

2018-04-01

Underfill is typically applied in flip chips to increase the reliability of the electronic packagings. In this paper, the evolution of the melt-front shape of the capillary-driven underfill flow is studied through 3D numerical analysis. Two different models, the prevailing surface force model and the capillary model based on the wetted wall boundary condition, are introduced to test their applicability, where level set method is used to track the interface of the two phase flow. The comparison between the simulation results and experimental data indicates that, the surface force model produces better prediction on the melt-front shape, especially in the central area of the flip chip. Nevertheless, the two above models cannot simulate properly the racing effect phenomenon that appears during underfill encapsulation. A novel ‘dynamic pressure boundary condition’ method is proposed based on the validated surface force model. Utilizing this approach, the racing effect phenomenon is simulated with high precision. In addition, a linear relationship is derived from this model between the flow front location at the edge of the flip chip and the filling time. Using the proposed approach, the impact of the underfill-dispensing length on the melt-front shape is also studied.

Field-Scale Modeling of Local Capillary Trapping During CO2 Injection into a Saline Aquifer

NASA Astrophysics Data System (ADS)

Ren, B.; Lake, L. W.; Bryant, S. L.

2015-12-01

Local capillary trapping is the small-scale (10-2 to 10+1 m) CO2 trapping that is caused by the capillary pressure heterogeneity. The benefit of LCT, applied specially to CO2 sequestration, is that saturation of stored CO2 is larger than the residual gas, yet these CO2 are not susceptible to leakage through failed seals. Thus quantifying the extent of local capillary trapping is valuable in design and risk assessment of geologic storage projects. Modeling local capillary trapping is computationally expensive and may even be intractable using a conventional reservoir simulator. In this paper, we propose a novel method to model local capillary trapping by combining geologic criteria and connectivity analysis. The connectivity analysis originally developed for characterizing well-to-reservoir connectivity is adapted to this problem by means of a newly defined edge weight property between neighboring grid blocks, which accounts for the multiphase flow properties, injection rate, and gravity effect. Then the connectivity is estimated from shortest path algorithm to predict the CO2 migration behavior and plume shape during injection. A geologic criteria algorithm is developed to estimate the potential local capillary traps based only on the entry capillary pressure field. The latter is correlated to a geostatistical realization of permeability field. The extended connectivity analysis shows a good match of CO2 plume computed by the full-physics simulation. We then incorporate it into the geologic algorithm to quantify the amount of LCT structures identified within the entry capillary pressure field that can be filled during CO2 injection. Several simulations are conducted in the reservoirs with different level of heterogeneity (measured by the Dykstra-Parsons coefficient) under various injection scenarios. We find that there exists a threshold Dykstra-Parsons coefficient, below which low injection rate gives rise to more LCT; whereas higher injection rate increases LCT in heterogeneous reservoirs. Both the geologic algorithm and connectivity analysis are very fast; therefore, the integrated methodology can be used as a quick tool to estimate local capillary trapping. It can also be used as a potential complement to the full-physics simulation to evaluate safe storage capacity.

Lennard-Jones fluids in two-dimensional nano-pores. Multi-phase coexistence and fluid structure

NASA Astrophysics Data System (ADS)

Yatsyshin, Petr; Savva, Nikos; Kalliadasis, Serafim

2014-03-01

We present a number of fundamental findings on the wetting behaviour of nano-pores. A popular model for fluid confinement is a one-dimensional (1D) slit pore formed by two parallel planar walls and it exhibits capillary condensation (CC): a first-order phase transition from vapour to capillary-liquid (Kelvin shift). Capping such a pore at one end by a third orthogonal wall forms a prototypical two-dimensional (2D) pore. We show that 2D pores possess a wetting temperature such that below this temperature CC remains of first order, above it becomes a continuous phase transition manifested by a slab of capillary-liquid filling the pore from the capping wall. Continuous CC exhibits hysteresis and can be preceded by a first-order capillary prewetting transition. Additionally, liquid drops can form in the corners of the 2D pore (remnant of 2D wedge prewetting). The three fluid phases, vapour, capillary-liquid slab and corner drops, can coexist at the pore triple point. Our model is based on the statistical mechanics of fluids in the density functional formulation. The fluid-fluid and fluid-substrate interactions are dispersive. We analyze in detail the microscopic fluid structure, isotherms and full phase diagrams. Our findings also suggest novel ways to control wetting of nano-pores. We are grateful to the European Research Council via Advanced Grant No. 247031 for support.

Impact of polymer film thickness and cavity size on polymer flow during embossing : towards process design rules for nanoimprint lithography.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schunk, Peter Randall; King, William P.; Sun, Amy Cha-Tien

2006-08-01

This paper presents continuum simulations of polymer flow during nanoimprint lithography (NIL). The simulations capture the underlying physics of polymer flow from the nanometer to millimeter length scale and examine geometry and thermophysical process quantities affecting cavity filling. Variations in embossing tool geometry and polymer film thickness during viscous flow distinguish different flow driving mechanisms. Three parameters can predict polymer deformation mode: cavity width to polymer thickness ratio, polymer supply ratio, and Capillary number. The ratio of cavity width to initial polymer film thickness determines vertically or laterally dominant deformation. The ratio of indenter width to residual film thickness measuresmore » polymer supply beneath the indenter which determines Stokes or squeeze flow. The local geometry ratios can predict a fill time based on laminar flow between plates, Stokes flow, or squeeze flow. Characteristic NIL capillary number based on geometry-dependent fill time distinguishes between capillary or viscous driven flows. The three parameters predict filling modes observed in published studies of NIL deformation over nanometer to millimeter length scales. The work seeks to establish process design rules for NIL and to provide tools for the rational design of NIL master templates, resist polymers, and process parameters.« less

Proof Of Concept of Integrated Load Measurement in 3D Printed Structures

DOE PAGES

Hinderdael, Michael; Strantza, Maria; De Baere, Dieter; ...

2017-02-09

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externallymore » mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain).« less

Proof Of Concept of Integrated Load Measurement in 3D Printed Structures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hinderdael, Michael; Strantza, Maria; De Baere, Dieter

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externallymore » mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain).« less

Proof of Concept of Integrated Load Measurement in 3D Printed Structures

PubMed Central

Hinderdael, Michaël; Jardon, Zoé; Lison, Margot; De Baere, Dieter; Devesse, Wim; Strantza, Maria; Guillaume, Patrick

2017-01-01

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externally mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain). PMID:28208779

Crossover from capillary fingering to viscous fingering in a rough fracture

NASA Astrophysics Data System (ADS)

Hu, R.; Chen, Y.; Wu, D. S.

2017-12-01

Controlled by the competition between capillary and viscous forces, the displacement patterns of one fluid displacing another more viscous one exhibit capillary fingering, viscous fingering, and the crossover between the two. Although extensive studies have investigated viscous and capillary fingerings in porous and fractured media, a few studies focused on the crossover in rough fractures, and how viscous and capillary forces affect the crossover remains unclear. Using a transparent fracture visualization system, we studied how the competition impacts the crossover in a horizontal rough fracture. Drainage experiments of water displacing oil were conducted at seven flow rates (capillary number log10Ca ranging from -7.07 to -3.07) and four viscosity ratios (M = 1/1000, 1/500, 1/100 and 1/50). We consistently observed lower invading fluid saturations in the crossover zone. In addition, we proposed a phase diagram for the displacement patterns in a rough fracture that is consistent with similar studies in porous media. Based on real-time imaging and statistical analysis of the invasion morphology, we showed that the competition between the capillary and viscous forces is responsible for the saturation reduction in the crossover zone. In this zone, finger propagation toward the outlet (characteristic of viscous fingering) as well as void-filling in the transverse and backward directions (characteristic of capillary fingering), are both suppressed. Therefore, the invading fluid tends to occupy larger apertures with higher characteristic front velocity, promoting void-filling toward the outlet with thinner finger growth and resulting in a larger volume of defending fluid left behind.

Investigation of capillary nanosecond discharges in air at moderate pressure: comparison of experiments and 2D numerical modelling

NASA Astrophysics Data System (ADS)

Klochko, Andrei V.; Starikovskaia, Svetlana M.; Xiong, Zhongmin; Kushner, Mark J.

2014-09-01

Nanosecond electrical discharges in the form of ionization waves are of interest for rapidly ionizing and exciting complex gas mixtures to initiate chemical reactions. Operating with a small discharge tube diameter can significantly increase the specific energy deposition and so enable optimization of the initiation process. Analysis of the uniformity of energy release in small diameter capillary tubes will aid in this optimization. In this paper, results for the experimentally derived characteristics of nanosecond capillary discharges in air at moderate pressure are presented and compared with results from a two-dimensional model. The quartz capillary tube, having inner and outer diameters of 1.5 and 3.4 mm, is about 80 mm long and filled with synthetic dry air at 27 mbar. The capillary tube with two electrodes at the ends is inserted into a break of the central wire of a long coaxial cable. A metal screen around the tube is connected to the cable ground shield. The discharge is driven by a 19 kV 35 ns voltage pulse applied to the powered electrode. The experimental measurements are conducted primarily by using a calibrated capacitive probe and back current shunts. The numerical modelling focuses on the fast ionization wave (FIW) and the plasma properties in the immediate afterglow after the conductive plasma channel has been established between the two electrodes. The FIW produces a highly focused region of electric field on the tube axis that sustains the ionization wave that eventually bridges the electrode gap. Results from the model predict FIW propagation speed and current rise time that agree with the experiment.

Confinement-Driven Phase Separation of Quantum Liquid Mixtures

NASA Astrophysics Data System (ADS)

Prisk, T. R.; Pantalei, C.; Kaiser, H.; Sokol, P. E.

2012-08-01

We report small-angle neutron scattering studies of liquid helium mixtures confined in Mobil Crystalline Material-41 (MCM-41), a porous silica glass with narrow cylindrical nanopores (d=3.4nm). MCM-41 is an ideal model adsorbent for fundamental studies of gas sorption in porous media because its monodisperse pores are arranged in a 2D triangular lattice. The small-angle scattering consists of a series of diffraction peaks whose intensities are determined by how the imbibed liquid fills the pores. Pure He4 adsorbed in the pores show classic, layer-by-layer film growth as a function of pore filling, leaving the long range symmetry of the system intact. In contrast, the adsorption of He3-He4 mixtures produces a structure incommensurate with the pore lattice. Neither capillary condensation nor preferential adsorption of one helium isotope to the pore walls can provide the symmetry-breaking mechanism. The scattering is consistent with the formation of randomly distributed liquid-liquid microdomains ˜2.3nm in size, providing evidence that confinement in a nanometer scale capillary can drive local phase separation in quantum liquid mixtures.

Capillary pumping independent of the liquid surface energy and viscosity

NASA Astrophysics Data System (ADS)

Guo, Weijin; Hansson, Jonas; van der Wijngaart, Wouter

2018-03-01

Capillary pumping is an attractive means of liquid actuation because it is a passive mechanism, i.e., it does not rely on an external energy supply during operation. The capillary flow rate generally depends on the liquid sample viscosity and surface energy. This poses a problem for capillary-driven systems that rely on a predictable flow rate and for which the sample viscosity or surface energy are not precisely known. Here, we introduce the capillary pumping of sample liquids with a flow rate that is constant in time and independent of the sample viscosity and sample surface energy. These features are enabled by a design in which a well-characterized pump liquid is capillarily imbibed into the downstream section of the pump and thereby pulls the unknown sample liquid into the upstream pump section. The downstream pump geometry is designed to exert a Laplace pressure and fluidic resistance that are substantially larger than those exerted by the upstream pump geometry on the sample liquid. Hence, the influence of the unknown sample liquid on the flow rate is negligible. We experimentally tested pumps of the new design with a variety of sample liquids, including water, different samples of whole blood, different samples of urine, isopropanol, mineral oil, and glycerol. The capillary filling speeds of these liquids vary by more than a factor 1000 when imbibed to a standard constant cross-section glass capillary. In our new pump design, 20 filling tests involving these liquid samples with vastly different properties resulted in a constant volumetric flow rate in the range of 20.96-24.76 μL/min. We expect this novel capillary design to have immediate applications in lab-on-a-chip systems and diagnostic devices.

Visualizing and quantifying the crossover from capillary fingering to viscous fingering in a rough fracture

NASA Astrophysics Data System (ADS)

Chen, Yi-Feng; Fang, Shu; Wu, Dong-Sheng; Hu, Ran

2017-09-01

Immiscible fluid-fluid displacement in permeable media is important in many subsurface processes, including enhanced oil recovery and geological CO2 sequestration. Controlled by capillary and viscous forces, displacement patterns of one fluid displacing another more viscous one exhibit capillary and viscous fingering, and crossover between the two. Although extensive studies investigated viscous and capillary fingering in porous media, a few studies focused on the crossover in rough fractures, and how viscous and capillary forces affect the crossover remains unclear. Using a transparent fracture-visualization system, we studied how the two forces impact the crossover in a horizontal rough fracture. Drainage experiments of water displacing oil were conducted at seven flow rates (capillary number log10Ca ranging from -7.07 to -3.07) and four viscosity ratios (M=1/1000,1/500,1/100 and 1/50). We consistently observed lower invading fluid saturations in the crossover zone. We also proposed a phase diagram for the displacement patterns in a rough fracture that is consistent with similar studies in porous media. Based on real-time imaging and statistical analysis of the invasion morphology, we showed that the competition between capillary and viscous forces is responsible for the saturation reduction in the crossover zone. In this zone, finger propagation toward the outlet (characteristic of viscous fingering) as well as void-filling in the transverse/backward directions (characteristic of capillary fingering), are both suppressed. Therefore, the invading fluid tends to occupy larger apertures with higher characteristic front velocity, promoting void-filling toward the outlet with thinner finger growth and resulting in a larger volume of defending fluid left behind.

Fabrication of self-enclosed nanochannels based on capillary-pressure balance mechanism

NASA Astrophysics Data System (ADS)

Kou, Yu; Sang, Aixia; Li, Xin; Wang, Xudi

2017-10-01

Polymer-based micro/nano fluidic devices are becoming increasingly important to biological applications and fluidic control. In this paper, we propose a self-enclosure method for the fabrication of large-area nanochannels without external force by using a capillary-pressure balance mechanism. The melt polymer coated on the nanogrooves fills into the trenches inevitably and the air in the trenches is not excluded but compressed, which leads to an equilibrium state between pressure of the trapped air and capillary force of melt polymer eventually, resulting in the channels’ formation. A pressure balance model was proposed to elucidate the unique self-sealing phenomenon and the criteria for the design and construction of sealed channels was discussed. According to the bonding mechanism investigated using the volume of fluid (VOF) simulation and experiments, we can control the dimension of sealed channels by varying the baking condition. This fabrication technique has great potential for low-cost and mass production of polymeric-based micro/nano fluidic devices.

An Amorphous Network Model for Capillary Flow and Dispersion in a Partially Saturated Porous Medium

NASA Astrophysics Data System (ADS)

Simmons, C. S.; Rockhold, M. L.

2013-12-01

Network models of capillary flow are commonly used to represent conduction of fluids at pore scales. Typically, a flow system is described by a regular geometric lattice of interconnected tubes. Tubes constitute the pore throats, while connection junctions (nodes) are pore bodies. Such conceptualization of the geometry, however, is questionable for the pore scale, where irregularity clearly prevails, although prior published models using a regular lattice have demonstrated successful descriptions of the flow in the bulk medium. Here a network is allowed to be amorphous, and is not subject to any particular lattice structure. Few network flow models have treated partially saturated or even multiphase conditions. The research trend is toward using capillary tubes with triangular or square cross sections that have corners and always retain some fluid by capillarity when drained. In contrast, this model uses only circular capillaries, whose filled state is controlled by a capillary pressure rule for the junctions. The rule determines which capillary participate in the flow under an imposed matric potential gradient during steady flow conditions. Poiseuille's Law and Laplace equation are used to describe flow and water retention in the capillary units of the model. A modified conjugate gradient solution for steady flow that tracks which capillary in an amorphous network contribute to fluid conduction was devised for partially saturated conditions. The model thus retains the features of classical capillary models for determining hydraulic flow properties under unsaturated conditions based on distribution of non-interacting tubes, but now accounts for flow exchange at junctions. Continuity of the flow balance at every junction is solved simultaneously. The effective water retention relationship and unsaturated permeability are evaluated for an extensive enough network to represent a small bulk sample of porous medium. The model is applied for both a hypothetically randomly generate network and for a directly measured porous medium structure, by means of xray-CT scan. A randomly generated network has the benefit of providing ensemble averages for sample replicates of a medium's properties, whereas network structure measurements are expected to be more predictive. Dispersion of solute in a network flow is calculate by using particle tracking to determine the travel time breakthrough between inflow and outflow boundaries. The travel time distribution can exhibit substantial skewness that reflects both network velocity variability and mixing dilution at junctions. When local diffusion is not included, and transport is strictly advective, then the skew breakthrough is not due to mobile-immobile flow region behavior. The approach of dispersivity to its asymptotic value with sample size is examined, and may be only an indicator of particular stochastic flow variation. It is not proven that a simplified network flow model can accurately predict the hydraulic properties of a sufficiently large-size medium sample, but such a model can at least demonstrate macroscopic flow resulting from the interaction of physical processes at pore scales.

[Is the use of plastic capillary tubes justified for blood gases analysis?].

PubMed

Daurès, Marie-Françoise; Bozonnat, Marie-Cécile; Cristol, Jean-Paul

2011-01-01

Some clinical units, such as neonatal or maternity units, preferentially use capillary tubes when analysing blood gases. Using glass tubes is delicate and nurses must recollect blood when breaking. In order to eliminate this problem, we tested flexible, plastic capillary tubes in both the above mentionned units and in our biochemistry laboratory. Each unit, where glass tubes were habitually used, tested 200 flexible, plastic capillary tubes. In addition, the nursing staffed filled out a questionnaire concerned tube usage. Both units clearly preferred using the flexible tubes. In the laboratory, results for blood gas analyses were compared between rigid glass and flexible plastic capillary tubes for 112 patients. Concordance tests did not showed significant differences between the two tube types, except for hematocrit and total haemoglobin. A questionnaire was also presented to the lab technician, who confirmed the easier usability of plastic capillary tubes.

Cerebral Microcirculation and Oxygen Tension in the Human Secondary Cortex

PubMed Central

Linninger, A. A.; Gould, I. G.; Marinnan, T.; Hsu, C.-Y.; Chojecki, M.; Alaraj, A.

2013-01-01

The three-dimensional spatial arrangement of the cortical microcirculatory system is critical for understanding oxygen exchange between blood vessels and brain cells. A three-dimensional computer model of a 3 × 3 × 3 mm3 subsection of the human secondary cortex was constructed to quantify oxygen advection in the microcirculation, tissue oxygen perfusion, and consumption in the human cortex. This computer model accounts for all arterial, capillary and venous blood vessels of the cerebral microvascular bed as well as brain tissue occupying the extravascular space. Microvessels were assembled with optimization algorithms emulating angiogenic growth; a realistic capillary bed was built with space filling procedures. The extravascular tissue was modeled as a porous medium supplied with oxygen by advection–diffusion to match normal metabolic oxygen demand. The resulting synthetic computer generated network matches prior measured morphometrics and fractal patterns of the cortical microvasculature. This morphologically accurate, physiologically consistent, multi-scale computer network of the cerebral microcirculation predicts the oxygen exchange of cortical blood vessels with the surrounding gray matter. Oxygen tension subject to blood pressure and flow conditions were computed and validated for the blood as well as brain tissue. Oxygen gradients along arterioles, capillaries and veins agreed with in vivo trends observed recently in imaging studies within experimental tolerances and uncertainty. PMID:23842693

Silicon deposition in nanopores using a liquid precursor.

PubMed

Masuda, Takashi; Tatsuda, Narihito; Yano, Kazuhisa; Shimoda, Tatsuya

2016-11-22

Techniques for depositing silicon into nanosized spaces are vital for the further scaling down of next-generation devices in the semiconductor industry. In this study, we filled silicon into 3.5-nm-diameter nanopores with an aspect ratio of 70 by exploiting thermodynamic behaviour based on the van der Waals energy of vaporized cyclopentasilane (CPS). We originally synthesized CPS as a liquid precursor for semiconducting silicon. Here we used CPS as a gas source in thermal chemical vapour deposition under atmospheric pressure because vaporized CPS can fill nanopores spontaneously. Our estimation of the free energy of CPS based on Lifshitz van der Waals theory clarified the filling mechanism, where CPS vapour in the nanopores readily undergoes capillary condensation because of its large molar volume compared to those of other vapours such as water, toluene, silane, and disilane. Consequently, a liquid-specific feature was observed during the deposition process; specifically, condensed CPS penetrated into the nanopores spontaneously via capillary force. The CPS that filled the nanopores was then transformed into solid silicon by thermal decomposition at 400 °C. The developed method is expected to be used as a nanoscale silicon filling technology, which is critical for the fabrication of future quantum scale silicon devices.

Silicon deposition in nanopores using a liquid precursor

NASA Astrophysics Data System (ADS)

Masuda, Takashi; Tatsuda, Narihito; Yano, Kazuhisa; Shimoda, Tatsuya

2016-11-01

Techniques for depositing silicon into nanosized spaces are vital for the further scaling down of next-generation devices in the semiconductor industry. In this study, we filled silicon into 3.5-nm-diameter nanopores with an aspect ratio of 70 by exploiting thermodynamic behaviour based on the van der Waals energy of vaporized cyclopentasilane (CPS). We originally synthesized CPS as a liquid precursor for semiconducting silicon. Here we used CPS as a gas source in thermal chemical vapour deposition under atmospheric pressure because vaporized CPS can fill nanopores spontaneously. Our estimation of the free energy of CPS based on Lifshitz van der Waals theory clarified the filling mechanism, where CPS vapour in the nanopores readily undergoes capillary condensation because of its large molar volume compared to those of other vapours such as water, toluene, silane, and disilane. Consequently, a liquid-specific feature was observed during the deposition process; specifically, condensed CPS penetrated into the nanopores spontaneously via capillary force. The CPS that filled the nanopores was then transformed into solid silicon by thermal decomposition at 400 °C. The developed method is expected to be used as a nanoscale silicon filling technology, which is critical for the fabrication of future quantum scale silicon devices.

Capillary electrochromatography and capillary electrochromatography-electrospray mass spectrometry for the separation of non-steroidal anti-inflammatory drugs.

PubMed

Desiderio, C; Fanali, S

2000-10-20

In this study capillary electrochromatography (CEC) was utilized for the separation of ten non-steroidal anti-inflammatory drugs (NSAIDs). Experiments were carried out in a commercially available CE instrument using a packed capillary with RP-18 silica particles where the stationary phase completely filled the capillary. The mobile phase consisted of a mixture of ammonium formate buffer pH 2.5 and acetonitrile. Selectivity and resolution were studied changing the pH and the concentration of the buffer, the acetonitrile content mobile phase and the capillary temperature. The optimum experimental conditions for CEC separation of the studied drug mixture were found using 50 mM ammonium formate pH 2.5-acetonitrile (40:60) at 25 degrees C. The CEC capillary was coupled to an electrospray mass spectrometer for the characterization of the NSAIDs. A mobile phase composed by the same buffer but with a higher concentration of acetonitrile (90%) was used in order to speed up the separation of analytes.

Capillary reference half-cell

DOEpatents

Hall, Stephen H.

1996-01-01

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods.

Capillary reference half-cell

DOEpatents

Hall, S.H.

1996-02-13

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods. 11 figs.

Fetal villosity and microvasculature of the bovine placentome in the second half of gestation

PubMed Central

LEISER, R.; KREBS, C.; KLISCH, K.; EBERT, B.; DANTZER, V.; SCHULER, G.; HOFFMANN, B.

1997-01-01

The architecture of the fetal villous tree and its vasculature in the bovine placentome were studied in the second half of gestation using both conventional histology and histology of ink-filled blood vessels. These were compared with corrosion casts of plastic fillings of the vasculature, prepared for scanning electron microscopy. This combination of morphological methods allows perception of the villous tree throughout gestation from broad-conical to tall-conical form where branch ramification occurs mainly at right angles to the stem. The stem villus typically contains a single central artery and several peripheral veins arranged in parallel. The proximal branches to the stem, the intermediate villi, contain a central arteriole and accompanying venules. The distal branches, the terminal villi, enclose capillary convolutions which consist of an afferent arterial capillary limb, capillary loops and efferent venous capillary limbs. Vascular interconnections exist within the terminal villi, as capillaries or venules between the capillary convolutions, serially bridging them in up to 5 places, and as capillary anastomoses between the capillary loops. Coiling and sinusoidal dilatations of these loops develop near the end of gestation. The intraplacentomal rearrangement of villous trees with progressive gestation and their morphological vascular adaptations are discussed in relation to placental function, including the ever increasing need for transplacental substance exchange. This adaptation allows the blood to traverse the shortest possible arterioarteriolar route to the periphery of the trees where exchange takes place. The need for an increasing blood flow stimulates capillary growth and at the same time optimises the blood flow reaching the placental barrier represented by the vessel cast surface. The capillaries also carry the blood back into the very voluminous system of venules and veins where back diffusion may occur. The total volume of terminal villi of bovine placentome, the ‘working part’ of villous trees, hence distinctly increases with respect to the stem and intermediate villi, the ‘supplying part’ of the villous tree. In morphological terms the efficiency of the bovine transplacental diffusional exchange is higher than in the closely related ‘co-ruminants’ sheep and goats and distinctly higher when compared with the human placenta. PMID:9449071

Method of filling a microchannel separation column

DOEpatents

Arnold, Don W.

2002-01-01

A method for packing a stationary phase into a small diameter fluid passageway or flow channel. Capillary action is employed to distribute a stationary phase uniformly along both the length and diameter of the flow channel. The method disclosed here: 1) eliminates the need for high pressure pumps and fittings and the safety hazards associated therewith; 2) allows the use of readily available commercial microparticles, either coated or uncoated, as the stationary phase; 3) provides for different types of particles, different particle sizes, and different particle size distributions to be packed in sequence, or simultaneously; 4) eliminates the need for plugging the flow channel prior to adding the stationary phase to retain the packing particles; and 5) many capillaries can be filled simultaneously.

Capillary-Effect Root-Environment System

NASA Technical Reports Server (NTRS)

Wright, Bruce D.

1991-01-01

Capillary-effect root-environment system (CERES) is experimental apparatus for growing plants in nutrient solutions. Solution circulated at slight tension in cavity filled with plastic screen and covered by porous plastic membrane. By adsorptive attraction, root draws solution through membrane. Conceived for use in microgravity of space, also finds terrestrial application in germinating seedlings, because it protects them from extremes of temperature, moisture, and soil pH and from overexposure to fertilizers and herbicides.

Into the theory of the partial-filling affinity capillary electrophoresis and the determination of apparent stability constants of analyte-ligand complexes.

PubMed

Ansorge, Martin; Dubský, Pavel; Ušelová, Kateřina

2018-03-01

The partial-filling affinity capillary electrophoresis (pf-ACE) works with a ligand present in a background electrolyte that forms a weak complex with an analyte. In contrast to a more popular mobility-shift affinity capillary electrophoresis, only a short plug of the ligand is introduced into a capillary in the pf-ACE. Both methods can serve for determining apparent stability constants of the formed complexes but this task is hindered in the pf-ACE by the fact that the analyte spends only a part of its migration time in a contact with the ligand. In 1998, Amini and Westerlund published a linearization strategy that allows for extracting an effective mobility of an analyte in the presence of a neutral ligand out of the pf-ACE data. The main purpose of this paper is to show that the original formula is only approximate. We derive a new formula and demonstrate its applicability by means of computer simulations. We further inspect several strategies of data processing in the pf-ACE regarding a risk of an error propagation. This establishes a good practice of determining apparent stability constants of analyte-ligand complexes by means of the pf-ACE. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Method development of enantiomer separations by affinity capillary electrophoresis, cyclodextrin electrokinetic chromatography and capillary electrophoresis-mass spectrometry.

PubMed

Tanaka, Yoshihide

2002-07-01

Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated from our study in this review article. In the enantiomer separations by affinity CE, the deterioration of detection sensitivity was observed under high concentration of the protein in running solutions. The partial filling technique was practically useful to solve the serious problem. It allowed operation at high protein concentrations, such as 500 mumol/L, without the detection problem. Charged CDs had several advantages for the enantiomer separations over neutral ones. Strong electrostatic interactions between a charged CD and oppositely charged analytes should be effective for the formation of the complex. A large difference in electrophoretic mobility between the free analyte and the inclusion complex should also enhance the enantiomeric resolution. In CE-mass spectrometry (CE-MS), the partial filling technique was applied to avoid the introduction of nonvolatile chiral selectors into the CE-MS interface. By replacing the nonvolatile electrolytes in the running buffer by volatile ones, the separation conditions employed in CE with the UV detection method could be transferred to CE-MS.

Behavior of CO2/water flow in porous media for CO2 geological storage.

PubMed

Jiang, Lanlan; Yu, Minghao; Liu, Yu; Yang, Mingjun; Zhang, Yi; Xue, Ziqiu; Suekane, Tetsuya; Song, Yongchen

2017-04-01

A clear understanding of two-phase fluid flow properties in porous media is of importance to CO 2 geological storage. The study visually measured the immiscible and miscible displacement of water by CO 2 using MRI (magnetic resonance imaging), and investigated the factor influencing the displacement process in porous media which were filled with quartz glass beads. For immiscible displacement at slow flow rates, the MR signal intensity of images increased because of CO 2 dissolution; before the dissolution phenomenon became inconspicuous at flow rate of 0.8mLmin -1 . For miscible displacement, the MR signal intensity decreased gradually independent of flow rates, because supercritical CO 2 and water became miscible in the beginning of CO 2 injection. CO 2 channeling or fingering phenomena were more obviously observed with lower permeable porous media. Capillary force decreases with increasing particle size, which would increase permeability and allow CO 2 and water to invade into small pore spaces more easily. The study also showed CO 2 flow patterns were dominated by dimensionless capillary number, changing from capillary finger to stable flow. The relative permeability curve was calculated using Brooks-Corey model, while the results showed the relative permeability of CO 2 slightly decreases with the increase of capillary number. Copyright © 2016 Elsevier Inc. All rights reserved.

Pulmonary diffusing capacity, capillary blood volume, and cardiac output during sustained microgravity

NASA Technical Reports Server (NTRS)

Prisk, G. K.; Guy, Harold J. B.; Elliott, Ann R.; Deutschman, Robert A., III; West, John B.

1993-01-01

We measured pulmonary diffusing capacity (DL), diffusing capacity per unit lung volume, pulmonary capillary blood volume (Vc), membrane diffusing capacity (Dm), pulmonary capillary blood flow or cardiac output (Qc), and cardiac stroke volume (SV) in four subjects exposed to nine days of microgravity. DL in microgravity was elevated compared with preflight standing values and was higher than preflight supine because of the elevation of both Vc and Dm. The elevation in Vc was comparable to that measured supine in 1 G, but the increase in Dm was in sharp contrast to the supine value. We postulate that, in 0 G, pulmonary capillary blood is evenly distributed throughout the lung, providing for uniform capillary filling, leading to an increase in the surface area available for diffusion. By contrast, in the supine 1-G state, the capillaries are less evenly filled, and although a similar increase in blood volume is observed, the corresponding increase in surface area does not occur. DL and its subdivisions showed no adaptive changes from the first measurement 24 h after the start of 0 G to eight days later. Similarly, there were no trends in the postflight data, suggesting that the principal mechanism of these changes was gravitational. The increase in Dm suggests that subclinical pulmonary edema did not result from exposure to 0 G. Qc was modestly increased inflight and decreased postflight compared with preflight standing. Compared with preflight standing, SV was increased 46 percent inflight and decreased 14 percent in the 1st week postflight. There were temporal changes in Qc and SV during 0 G, with the highest values recorded at the first measurement, 24 h into the flight. The lowest values of Qc and SV occurred on the day of return.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe

NASA Astrophysics Data System (ADS)

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H.

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp = 200…600 μm, porosity ε = 0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol) = 0 after t = 6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe.

PubMed

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp=200...600 μm, porosity ε=0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol)=0 after t=6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest. Copyright © 2015 Elsevier B.V. All rights reserved.

Intermittent KoldBlue cryotherapy of 3x10 min changes mid-portion Achilles tendon microcirculation.

PubMed

Knobloch, Karsten; Grasemann, Ruth; Spies, Marcus; Vogt, Peter M

2007-06-01

Neovascularisation and microcirculatory changes have been reported in Achilles tendinopathy. Cryotherapy and compression, as part of a rest, ice, compression and elevation regimen, are shown to decrease pain and improve function. However, the microcirculatory changes following a given dosage of cryotherapy on mid-portion Achilles tendon remain unclear. Prospective clinical cohort study, level of evidence 2. 30 people (12 males, 33 (SD 12) years, body mass index 25.6 (5.3) kg/m2) were included in the cohort. 3x10 min KoldBlue ankle-cooling bandages were applied and microcirculation of Achilles tendon mid-portion was real-time and continuously assessed using a laser-Doppler-spectrophotometry system (O2C, Germany). Superficial capillary blood flow was reduced from 42 to 6, 5 and 3 relative units (rU) in the first, second and third cryotherapy periods, respectively (-65%, p = 0.001), with no significant capillary hyperaemia. Deep capillary tendon blood flow was reduced from 180 to 82, 53 and 52 rU (-71%, p = 0.001) within 6-9 min of application without hyperaemia. Superficial tendon oxygen saturation dropped significantly from 43% to 26%, 18% and 11% (p = 0.001) after repetitive cryotherapy, with persisting increase of tendon oxygenation during rewarming (51%, 49% and 54%, p = 0.077) up to 27% of the baseline level. At 8 mm tendon depth, cryotherapy preserved local oxygenation. Relative postcapillary venous tendon filling pressures were favourably reduced from 41 (11) to 31, 28 and 26 rU (-36%, p = 0.001) superficially and from 56 (11) to 45, 46 and 48 rU (-18%, p = 0.001) in deep capillary blood flow during cryotherapy, facilitating capillary venous clearance. Intermittent cryotherapy of 3x10 min significantly decreases local Achilles tendon mid-portion capillary blood flow by 71%. Within 2 min of rewarming, tendon oxygen saturation is re-established following cryotherapy. Postcapillary venous filling pressures are reduced during cryotherapy, favouring capillary venous outflow of the healthy Achilles tendon.

Optoelectronic Capillary Sensors in Microfluidic and Point-of-Care Instrumentation

PubMed Central

Borecki, Michał; Korwin-Pawlowski, Michael L.; Beblowska, Maria; Szmidt, Jan; Jakubowski, Andrzej

2010-01-01

This paper presents a review, based on the published literature and on the authors’ own research, of the current state of the art of fiber-optic capillary sensors and related instrumentation as well as their applications, with special emphasis on point-of-care chemical and biochemical sensors, systematizing the various types of sensors from the point of view of the principles of their construction and operation. Unlike classical fiber-optic sensors which rely on changes in light propagation inside the fiber as affected by outside conditions, optical capillary sensors rely on changes of light transmission in capillaries filled with the analyzed liquid, which opens the possibility of interesting new applications, while raising specific issues relating to the construction, materials and instrumentation of those sensors. PMID:22319325

Capillary Tube and Thermostatic Expansion Valve Comparative Analysis in Water Chiller Air Conditioning

NASA Astrophysics Data System (ADS)

Wijaya Sunu, Putu; Made Rasta, I.; Anakottapary, Daud Simon; Made Suarta, I.; Cipta Santosa, I. D. M.

2018-01-01

The aims of this study to compares the performance characteristics of a water chiller air conditioning simulation equipped with thermostatic expansion valve (TEV) with those of a capillary tube. Water chiller system filled with the same charge of refrigerant. Comparative analyses were performed based on coefficient of performance (COP) and performance parameter of the refrigeration system, carried out at medium cooling load level with the ambient temperature of 29-31°C, constant compressor speed and fixed chilled water volume flowrate at 15 lpm. It was shown that the TEV system showed better energy consumption compared to that of capillary tube. From the coefficient of performance perspective, the thermostatic expansion valve system showed higher COP (± 21.4%) compared to that of capillary tube system.

Control of electroosmosis in coated quartz capillaries

NASA Technical Reports Server (NTRS)

Herren, Blair J.; Van Alstine, James; Snyder, Robert S.; Shafer, Steven G.; Harris, J. Milton

1987-01-01

The effectiveness of various coatings for controlling the electroosmotic fluid flow that hinders electrophoretic processes is studied using analytical particle microelectrophoresis. The mobilities of 2-micron diameter glass and polystyrene latex spheres (exhibiting both negative and zero effective surface charge) were measured in 2-mm diameter quartz capillaries filled with NaCl solutions within the 3.5-7.8 pH range. It is found that capillary inner surface coatings using 5000 molecular weight (or higher) poly(ethylene glycol): significantly reduced electroosmosis within the selected pH range, were stable for long time periods, and appeared to be more effective than dextran, methylcellulose, or silane coatings.

Wettability Control on Fluid-Fluid Displacements in Patterned Microfluidics

NASA Astrophysics Data System (ADS)

Zhao, B.; Trojer, M.; Cueto-Felgueroso, L.; Juanes, R.

2014-12-01

Two-phase flow in porous media is important in many natural and industrial processes like geologic CO2 sequestration, enhanced oil recovery, and water infiltration in soil. While it is well known that the wetting properties of porous media can vary drastically depending on the type of media and the pore fluids, the effect of wettability on fluid displacement continues to challenge our microscopic and macroscopic descriptions. Here we study this problem experimentally, starting with the classic experiment of two-phase flow in a capillary tube. We image the shape of the meniscus and measure the associated capillary pressure for a wide range of capillary numbers. We confirm that wettability exerts a fundamental control on meniscus deformation, and synthesize new observations on the dependence of the dynamic capillary pressure on wetting properties (contact angle) and flow conditions (viscosity contrast and capillary number). We compare our experiments to a macroscopic phase-field model of two-phase flow. We use the insights gained from the capillary tube experiments to explore the viscous fingering instability in the Hele-Shaw geometry in the partial-wetting regime. A key difference between a Hele-Shaw cell and a porous medium is the existence of micro-structures (i.e. pores and pore throats). To investigate how these micro-structrues impact fluid-fluid displacement, we conduct experiments on a planar microfluidic device patterned with vertical posts. We track the evolution of the fluid-fluid interface and elucidate the impact of wetting on the cooperative nature of fluid displacement during pore invasion events. We use the insights gained from the capillary tube and patterned microfluidics experiments to elucidate the effect of wetting properties on viscous fingering and capillary fingering in a Hele-Shaw cell filled with glass beads, where we observe a contact-angle-dependent stabilizing behavior for the emerging flow instabilities, as the system transitions from drainage to imbibition.

A novel pneumatic micropipette aspiration method using a balance pressure model.

PubMed

Zhao, Qili; Wu, Ming; Cui, Maosheng; Qin, Yanding; Yu, Jin; Sun, Mingzhu; Zhao, Xin; Feng, Xizeng

2013-12-01

This paper presents a novel micropipette aspiration (MA) method based on a common pneumatic micro-injection system. This method is the first to quantify the influence of capillary effect on aspiration pressure using a balance pressure model, and in return, uses the capillary effect to quantify the aspiration pressure. Subsequently, the seal between the cell and the micropipette is detected to judge and exclude the ineffective MA attempts. The rationality of the balance pressure model is validated by the designed micropipette-filling experiments. Through applied to elasticity-determination of the cells with different sizes, the feasibility and versatility of this MA method are proved. With abilities to quantify aspiration pressures and detect the seam between the cell and the micropipette, our method is expected to advance the application of the commercial pneumatic injector in the MA of cells. Moreover, with the quantified volume of the liquid entering into the micropipette during MA process, our method also has a potential applicability to the study of the permeability of the cell membrane in the future.

New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide

PubMed Central

Janković, Bojan; Janković, Marija; Nikolić, Bogdan; Dimkić, Ivica; Lalević, Blažo; Raičević, Vera

2017-01-01

Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant. PMID:28644899

New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide.

PubMed

Waisi, Hadi; Janković, Bojan; Janković, Marija; Nikolić, Bogdan; Dimkić, Ivica; Lalević, Blažo; Raičević, Vera

2017-01-01

Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant.

Characterizing the interaction between enantiomers of eight psychoactive drugs and highly sulfated-β-cyclodextrin by counter-current capillary electrophoresis.

PubMed

Asensi-Bernardi, Lucía; Escuder-Gilabert, Laura; Martín-Biosca, Yolanda; Sagrado, Salvador; Medina-Hernández, María José

2014-01-01

The estimation of apparent binding constants and limit mobilities of the complexes of the enantiomers that characterize the interaction of enantiomers with chiral selectors, in this case highly sulfated β-cyclodextrin, was approached using a simple and economic electrophoretic modality, the complete filling technique (CFT) in counter-current mode. The enantiomers of eight psychoactive drugs, four antihistamines (dimethindene, promethazine, orphenadrine and terfenadine) and four antidepressants (bupropion, fluoxetine, nomifensine and viloxazine) were separated for the first time for this cyclodextrin (CD). Estimations of thermodynamic and electrophoretic enantioselectivies were also performed. Results indicate that, in general, thermodynamic enantioselectivity is the main component explaining the high resolution found, but also one case suggests that electrophoretic enantioselectivity itself is enough to obtain a satisfactory resolution. CFT results advantageous compared with conventional capillary electrophoresis (CE) and partial filling technique (PFT) for the study of the interaction between drugs and chiral selectors. It combines the use of a simple fitting model (as in CE), when the enantiomers do not exit the chiral selector plug during the separation (i.e. mobility of electroosmotic flow larger than mobility of CD), and drastic reduction of the consumption (and cost; ~99.7%) of the CD reagent (as in PFT) compared with the conventional CE. Copyright © 2013 John Wiley & Sons, Ltd.

Wettability control on fluid-fluid displacements in patterned microfluidics and porous media

NASA Astrophysics Data System (ADS)

Juanes, Ruben; Trojer, Mathias; Zhao, Benzhong

2014-11-01

While it is well known that the wetting properties are critical in two-phase flows in porous media, the effect of wettability on fluid displacement continues to challenge our microscopic and macroscopic descriptions. Here we study this problem experimentally, starting with the classic experiment of two-phase flow in a capillary tube. We image the shape of the meniscus and measure the associated capillary pressure for a wide range of capillary numbers. We synthesize new observations on the dependence of the dynamic capillary pressure on wetting properties (contact angle) and flow conditions (viscosity contrast and capillary number). We then conduct experiments on a planar microfluidic device patterned with vertical posts. We track the evolution of the fluid-fluid interface and elucidate the impact of wetting on the cooperative nature of fluid displacement during pore invasion events. We use the insights gained from the capillary tube and patterned microfluidics experiments to elucidate the effect of wetting properties on viscous fingering and capillary fingering in a Hele-Shaw cell filled with glass beads, where we observe a contact-angle-dependent stabilizing behavior for the emerging flow instabilities, as the system transitions from drainage to imbibition.

Hydraulic droplet coarsening in open-channel capillaries

NASA Astrophysics Data System (ADS)

Warren, Patrick B.

2016-11-01

Over a range of liquid-solid contact angles, an open-channel capillary with curved or angled sides can show a maximum in the Laplace pressure as a function of the filling state. Examples include double-angle wedges, grooves scored into flat surfaces, steps on surfaces, and the groove between touching parallel cylinders. The liquid in such a channel exhibits a beading instability if the channel is filled beyond the Laplace pressure maximum. The subsequent droplet coarsening takes place by hydraulic transport through the connecting liquid columns that remain in the groove. A mean-field scaling argument predicts the characteristic droplet radius R ˜t1 /7 , as a function of time t . This is confirmed by one-dimensional simulations of the coarsening kinetics. Some remarks are also made on the spreading kinetics of an isolated drop deposited in such a channel.

Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation

NASA Astrophysics Data System (ADS)

Desrochers, Johanne; Vermette, Patrick; Fontaine, Réjean; Bérubé-Lauzière, Yves

2009-02-01

Current efforts in tissue engineering target the growth of 3D volumes of tissue cultures in bioreactor conditions. Fluorescence optical tomography has the potential to monitor cells viability and tissue growth non-destructively directly within the bioreactor via bio-molecular fluorescent labelling strategies. We currently work on developing the imaging instrumentation for tissue cultures in bioreactor conditions. Previously, we localized in 3D thin fluorescent-labelled capillaries in a cylindrically shaped bioreactor phantom containing a diffusive medium with our time-of-flight localization technique. Here, we present our first reconstruction results of the spatial distribution of fluorophore concentrations for labelled capillaries embedded in a bioreactor phantom.

Primary drainage in geological fractures: Effects of aperture variability and wettability

NASA Astrophysics Data System (ADS)

Yang, Z.; Méheust, Y.; Neuweiler, I.

2017-12-01

Understanding and controlling fluid-fluid displacement in porous and fractured media is a key asset for many practical applications, such as the geological storage of CO2, hydrocarbon recovery, groundwater remediation, etc. We numerically investigate fluid-fluid displacement in rough-walled fractures with a focus on the combined effect of wettability, the viscous contrast between the two fluids, and fracture surface topography on drainage patterns and interface growth. A model has been developed to simulate the dynamic displacement of one fluid by another immiscible one in a rough geological fracture; the model takes both capillary and viscous forces into account. Capillary pressures at the fluid-fluid interface are calculated based on the Young-Laplace equation using the two principal curvatures (aperture-induced curvature and in-plane curvature) [1], while viscous forces are calculated by continuously solving the fluid pressure field in the fracture. The aperture field of a fracture is represented by a spatially correlated random field, with a power spectral density of the fracture wall topographies scaling as a power law, and a cutoff wave-length above which the Fourier modes of the two walls are identical [2]. We consider flow scenarios with both rectangular and radial configurations. Results show that the model is able to produce displacement patterns of compact displacement, capillary fingering, and viscous fingering, as well as the transitions between them. Both reducing the aperture variability and increasing the contact angle (from drainage to weak imbibition) can stabilize the displacement due to the influence of the in-plane curvature, an effect analogous to that of the cooperative pore filling in porous media. These results suggest that for geometries typical of geological fractures we can extend the phase diagram in the parameter space of capillary number and mobility ratio by another dimension to take into account the combined effect of wettability and fracture aperture topography. References: [1] Yang, Z. et al. (2012), A generalized approach for estimation of in-plane curvature in invasion percolation models for drainage in fractures. Wat. Resour. Res., 48(9), W09507. [2] Yang, Z. et al. (2016), Fluid trapping during capillary displacement in fractures. Adv. Water Resour., 95, 264-275.

Drainage and impregnation capillary pressure curves calculated by the X-ray CT model of Berea sandstone using Lattice Boltzmann's method

NASA Astrophysics Data System (ADS)

Zakirov, T.; Galeev, A.; Khramchenkov, M.

2018-05-01

The study deals with the features of the technique for simulating the capillary pressure curves of porous media on their X-ray microtomographic images. The results of a computational experiment on the immiscible displacement of an incompressible fluid by another in the pore space represented by a digital image of the Berea sandstone are presented. For the mathematical description of two-phase fluid flow we use Lattice Boltzmann Equation (LBM), and phenomena at the fluids interface are described by the color-gradient model. Compared with laboratory studies, the evaluation of capillary pressure based on the results of a computational filtration experiment is a non-destructive method and has a number of advantages: the absence of labor for preparation of fluids and core; the possibility of modeling on the scale of very small core fragments (several mm), which is difficult to realize under experimental conditions; three-dimensional visualization of the dynamics of filling the pore space with a displacing fluid during drainage and impregnation; the possibility of carrying out multivariate calculations for specified parameters of multiphase flow (density and viscosity of fluids, surface tension, wetting contact angle). A satisfactory agreement of the capillary pressure curves during drainage with experimental results was obtained. It is revealed that with the increase in the volume of the digital image, the relative deviation of the calculated and laboratory data decreases and for cubic digital cores larger than 1 mm it does not exceed 5%. The behavior of the non-wetting fluid flow during drainage is illustrated. It is shown that flow regimes under which computational and laboratory experiments are performed the distribution of the injected phase in directions different from the gradient of the hydrodynamic drop, including the opposite ones, is characteristic. Experimentally confirmed regularities are obtained when carrying out calculations for drainage and imbibition at different values of interfacial tension. There is a close coincidence in the average diameters of permeable channels, estimated by capillary curves for different interfacial tension and pore network model. The differences do not exceed 15%.

Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer

DOEpatents

Burtis, Carl A.; Johnson, Wayne F.; Walker, William A.

1988-01-01

A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises (1) a whole blood sample disc, (2) a serum sample disc, (3) a sample preparation rotor, and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc in capillary tubes filled by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analytical rotor for analysis by conventional methods.

Optical fiber nanoprobe preparation for near-field optical microscopy by chemical etching under surface tension and capillary action.

PubMed

Mondal, Samir K; Mitra, Anupam; Singh, Nahar; Sarkar, S N; Kapur, Pawan

2009-10-26

We propose a technique of chemical etching for fabrication of near perfect optical fiber nanoprobe (NNP). It uses photosensitive single mode optical fiber to etch in hydro fluoric (HF) acid solution. The difference in etching rate for cladding and photosensitive core in HF acid solution creates capillary ring along core-cladding boundary under a given condition. The capillary ring is filled with acid solution due to surface tension and capillary action. Finally it creates near perfect symmetric tip at the apex of the fiber as the height of the acid level in capillary ring decreases while width of the ring increases with continuous etching. Typical tip features are short taper length (approximately 4 microm), large cone angle (approximately 38 degrees ), and small probe tip dimension (<100 nm). A finite difference time domain (FDTD) analysis is also presented to compare near field optics of the NNP with conventional nanoprobe (CNP). The probe may be ideal for near field optical imaging and sensor applications.

Drop Tower Experiments concerning Fluid Management under Microgravity

NASA Astrophysics Data System (ADS)

Gaulke, Diana; Dreyer, Michael

2012-07-01

Transport and positioning of liquid under microgravity is done utilizing capillary forces. Therefore, capillary transport processes have to be understood for a wide variety of space applications, ranging from propellant management in tanks of space transportation systems to eating and drinking devices for astronauts. There are two types of liquid transportation in microgravity using capillary forces. First, the driven liquid flow in open channels where the capillary forces at free surfaces ensure a gas and vapor free flow. Here it is important to know the limiting flow rate through such an open channel before the free surface collapses and gas is sucked into the channel. A number of different experiments at the drop tower Bremen, on sounding rockets and at the ISS have been conducted to analyse this phenomenon within different geometries. As result a geometry dependent theory for calculating the maximum flow rate has been found. On the other hand liquid positioning and transportation requires the capillary pressure of curved surfaces to achieve a liquid flow to a desired area. Especially for space applications the weight of structure has to be taken into account for development. For example liquid positioning in tanks can be achieved via a complicated set of structure filling the whole tank resulting in heavy devices not reasonable in space applications. Astrium developed in cooperation with ZARM a propellant management device much smaller than the tank volume and ensuring a gas and vapour free supply of propellant to the propulsion system. In the drop tower Bremen a model of this device was tested concerning different microgravity scenarios. To further decrease weight and ensure functionality within different scenarios structure elements are designed as perforated geometries. Capillary transport between perforated plates has been analyzed concerning the influence of geometrical pattern of perforations. The conducted experiments at the drop tower Bremen show the remarkable influence of perforations on the capillary transport capability.

Collisional radiative model of an argon atmospheric capillary surface-wave discharge

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yanguas-Gil, A.; Cotrino, J.; Gonzalez-Elipe, A.R.

2004-12-01

The characteristics of a microwave surface-wave sustained plasma operated at atmospheric pressure in an open-ended dielectric tube are investigated theoretically as a first step in the development of a self-consistent model for these discharges. The plasma column is sustained in flowing argon. A surface-wave discharge that fills the whole radial cross section of the discharge tube is considered. With experimental electron temperature profiles [Garcia et al., Spectrochim. Acta, Part B 55, 1733 (2000)] the numerical model is used to test the validity of the different approximations and to study the influence of the different kinetic processes and power loss mechanismsmore » on the discharge.« less

Stability of Buoyancy-Driven Gas Flow: Visualization of Coherent and Incoherent Gas Flow Patterns and Capillary Trapping

NASA Astrophysics Data System (ADS)

Geistlinger, H. W.; Samani, S.; Pohlert, M.; Jia, R.; Lazik, D.

2009-12-01

There are several mechanisms by which the CO2 can be stored: (1) In hydrodynamic trapping, the buoyant CO2 remains as a mobile fluid but is prevented from flowing back to the surface by an impermeable cap rock. (2) In solution trapping, CO2 dissolves into the brine, possibly enhanced by gravity instabilities due to the larger density of the brine-CO2 liquid mixture. (3) In mineral trapping, geochemical binding to the rock due to mineral precipitation. (4) In capillary trapping, the CO2 phase is disconnected into a coherent, mobile phase and an incoherent, immobile (trapped) phase. Recent analytical and numerical investigations [Juanes et al., 2006, 2009; Hesse et al., 2007 ] of buoyant-driven CO2-plume along a sloped aquifer are based on the following conceptual process model: (1) During the injection period, the less wetting CO2 displaces the more wetting brine in a drainage-like process. It is assumed that no capillary trapping occurs and that the CO2-network is coherent and driven both by the injection pressure and the buoyant pressure. Because of this coherence assumption a generalized Darcy-law can be used for the dynamics of the mobile, gaseous CO2-phase. (2) After injection the buoyant CO2 migrates laterally and upward, and water displaces CO2 at the trailing edge of the plume in an imbibition-like process. During this process, there are several physical mechanisms by which the water can displace the CO2 [Lenormand et al., 1983]. In addition to piston-type displacement, core-annular flow (also called: cooperative pore-body filling) may occur, i.e. the wetting phase moves along the walls and under certain conditions the CO2-core flow becomes unstable (snap-off). For water wet rocks, snap-off is the dominant mechanism [Al-Futaisi and Patzek, 2003; Valvatne and Blunt, 2004]. There seems to be consensus that the capillary trapping mechanism has a huge impact on the migration and distribution of CO2 which, in turn, affects the effectiveness of the other sequestration mechanisms. In order to investigate the stability of buoyancy-driven gas flow and the transition between coherent flow, incoherent flow, and their correlation to capillary trapping, we conducted high-resolution optical bench scale experiments. We observed a grain-size (dk) - and flow-rate (Q) dependent transition from incoherent to coherent flow. Based on core-annular flow (= cooperative pore-body filling), we propose a dynamic stability criterion that could describe our experimental results. Our experimental results for vertical gas flow support the experimental results by Lenormand et al. [1983] obtained for horizontal flow, if one takes into account that gravity leads to more unstable flow conditions. Our main results, which are in strong contradiction to the accepted conceptual model of the sloped aquifer, are: (1) Capillary Trapping can already occur during injection and at the front of the plume [Lazik et al., 2008] (2) Gas clusters or bubbles can be mobile (incoherent gas flow) and immobile (capillary trapping), and (3) Incoherent gas flow can not be described by a generalized Darcy law [Geistlinger et al., 2006, 2009].

A new model for studying the revascularization of skin grafts in vivo: the role of angiogenesis.

PubMed

Lindenblatt, Nicole; Calcagni, Maurizio; Contaldo, Claudio; Menger, Michael D; Giovanoli, Pietro; Vollmar, Brigitte

2008-12-01

Models of skin graft revascularization are based mostly on histologic evaluations but lack the possibility of analyzing the vascular biology in vivo. The aim of the present study was therefore to develop an animal model that allows continuous monitoring of the microcirculation during skin graft healing. Skin and subcutaneous tissue were removed from the back of dorsal skinfold chamber preparations in mice, leaving one layer of striated muscle and subcutaneous tissue as a wound bed (n = 5). A corresponding full-thickness skin graft was harvested from the groin and sutured into the defect in the back of the chamber. To study graft healing, repetitive intravital microscopy was performed during the first 10 days after engraftment. Capillary widening in the wound bed appeared at day 1 after grafting and increased until day 4. Capillary buds and sprouts first appeared at day 2. Blood filling of autochthonous graft capillaries occurred at day 3, resulting in almost complete restoration of the original skin microcirculation on day 5. This was achieved by interconnections between the microvasculature of the wound bed and the skin graft through a temporary angiogenic response. In principle, angiogenic blood vessel growth originated in the wound bed and was directed toward the graft. This new model allows for repetitive analysis of the microcirculation during skin graft healing. It provides ideal in vivo conditions to further delineate the exact mechanisms of blood vessel interconnection during the complex process of angiogenesis, and may also allow study of the vascularization of tissue-engineered skin substitutes.

Electron density measurement in gas discharge plasmas by optical and acoustic methods

NASA Astrophysics Data System (ADS)

Biagioni, A.; Anania, M. P.; Bellaveglia, M.; Chiadroni, E.; Cianchi, A.; Di Giovenale, D.; Di Pirro, G.; Ferrario, M.; Filippi, F.; Mostacci, A.; Pompili, R.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

2016-08-01

Plasma density represents a very important parameter for both laser wakefield and plasma wakefield acceleration, which use a gas-filled capillary plasma source. Several techniques can be used to measure the plasma density within a capillary discharge, which are mainly based on optical diagnostic methods, as for example the well-known spectroscopic method using the Stark broadening effect. In this work, we introduce a preliminary study on an alternative way to detect the plasma density, based on the shock waves produced by gas discharge in a capillary. Firstly, the measurements of the acoustic spectral content relative to the laser-induced plasmas by a solid target allowed us to understand the main properties of the acoustic waves produced during this kind of plasma generation; afterwards, we have extended such acoustic technique to the capillary plasma source in order to calibrate it by comparison with the stark broadening method.

Intermittent KoldBlue cryotherapy of 3×10 min changes mid‐portion Achilles tendon microcirculation

PubMed Central

Knobloch, Karsten; Grasemann, Ruth; Spies, Marcus; Vogt, Peter M

2007-01-01

Background Neovascularisation and microcirculatory changes have been reported in Achilles tendinopathy. Cryotherapy and compression, as part of a rest, ice, compression and elevation regimen, are shown to decrease pain and improve function. However, the microcirculatory changes following a given dosage of cryotherapy on mid‐portion Achilles tendon remain unclear. Study design Prospective clinical cohort study, level of evidence 2. Methods 30 people (12 males, 33 (SD 12) years, body mass index 25.6 (5.3) kg/m2) were included in the cohort. 3×10 min KoldBlue ankle‐cooling bandages were applied and microcirculation of Achilles tendon mid‐portion was real‐time and continuously assessed using a laser‐Doppler‐spectrophotometry system (O2C, Germany). Results Superficial capillary blood flow was reduced from 42 to 6, 5 and 3 relative units (rU) in the first, second and third cryotherapy periods, respectively (−65%, p = 0.001), with no significant capillary hyperaemia. Deep capillary tendon blood flow was reduced from 180 to 82, 53 and 52 rU (−71%, p = 0.001) within 6–9 min of application without hyperaemia. Superficial tendon oxygen saturation dropped significantly from 43% to 26%, 18% and 11% (p = 0.001) after repetitive cryotherapy, with persisting increase of tendon oxygenation during rewarming (51%, 49% and 54%, p = 0.077) up to 27% of the baseline level. At 8 mm tendon depth, cryotherapy preserved local oxygenation. Relative postcapillary venous tendon filling pressures were favourably reduced from 41 (11) to 31, 28 and 26 rU (−36%, p = 0.001) superficially and from 56 (11) to 45, 46 and 48 rU (−18%, p = 0.001) in deep capillary blood flow during cryotherapy, facilitating capillary venous clearance. Conclusion Intermittent cryotherapy of 3×10 min significantly decreases local Achilles tendon mid‐portion capillary blood flow by 71%. Within 2 min of rewarming, tendon oxygen saturation is re‐established following cryotherapy. Postcapillary venous filling pressures are reduced during cryotherapy, favouring capillary venous outflow of the healthy Achilles tendon. PMID:17138636

Three-dimensional spatial localization of thin fluorophore-filled capillaries in thick scattering media

NASA Astrophysics Data System (ADS)

Desrochers, Johanne; Vermette, Patrick; Fontaine, Réjean; Bérubé-Lauzière, Yves

2008-06-01

Fluorescence optical diffuse tomography (fDOT) is of much interest in molecular imaging to retrieve information from fluorescence signals emitted from specifically targeted bioprocesses deep within living tissues. An exciting application of fDOT is in the growing field of tissue engineering, where 3D non-invasive imaging techniques are required to ultimately grow 3D engineered tissues. Via appropriate labelling strategies and fluorescent probes, fDOT has the potential to monitor culture environment and cells viability non-destructively directly within the bioreactor environment where tissues are to be grown. Our ultimate objective is to image the formation of blood vessels in bioreactor conditions. Herein, we use a non-contact setup for small animal fDOT imaging designed for 3D light collection around the sample. We previously presented a time of flight approach using a numerical constant fraction discrimination technique to assign an early photons arrival time to every fluorescence time point-spread function collected around the sample. Towards bioreactor in-situ imaging, we have shown the capability of our approach to localize a fluorophore-filled 500 μm capillary immersed coaxially in a cylindrically shaped bioreactor phantom containing an absorbing/scattering medium representative of experiments on real tissue cultures. Here, we go one step further, and present results for the 3D localization of thinner indocyanine green labelled capillaries (250 μm and 360 μm inner diameter) immersed in the same phantom conditions and geometry but with different spatial configurations (10° and 30° capillary inclination).

Using Neutron Radiography to Quantify Water Transport and the Degree of Saturation in Entrained Air Cement Based Mortar

NASA Astrophysics Data System (ADS)

Lucero, Catherine L.; Bentz, Dale P.; Hussey, Daniel S.; Jacobson, David L.; Weiss, W. Jason

Air entrainment is commonly added to concrete to help in reducing the potential for freeze thaw damage. It is hypothesized that the entrained air voids remain unsaturated or partially saturated long after the smaller pores fill with water. Small gel and capillary pores in the cement matrix fill quickly on exposure to water, but larger pores (entrapped and entrained air voids) require longer times or other methods to achieve saturation. As such, it is important to quantitatively determine the water content and degree of saturation in air entrained cementitious materials. In order to further investigate properties of cement-based mortar, a model based on Beer's Law has been developed to interpret neutron radiographs. This model is a powerful tool for analyzing images acquired from neutron radiography. A mortar with a known volume of aggregate, water to cement ratio and degree of hydration can be imaged and the degree of saturation can be estimated.

Determination of blood oxygenation in the brain by time-resolved reflectance spectroscopy: influence of the skin, skull, and meninges

NASA Astrophysics Data System (ADS)

Hielscher, Andreas H.; Liu, Hanli; Wang, Lihong V.; Tittel, Frank K.; Chance, Britton; Jacques, Steven L.

1994-07-01

Near infrared light has been used for the determination of blood oxygenation in the brain but little attention has been paid to the fact that the states of blood oxygenation in arteries, veins, and capillaries differ substantially. In this study, Monte Carlo simulations for a heterogeneous system were conducted, and near infrared time-resolved reflectance measurements were performed on a heterogeneous tissue phantom model. The model was made of a solid polyester resin, which simulates the tissue background. A network of tubes was distributed uniformly through the resin to simulate the blood vessels. The time-resolved reflectance spectra were taken with different absorbing solutions filled in the network. Based on the simulation and experimental results, we investigated the dependence of the absorption coefficient obtained from the heterogeneous system on the absorption of the actual absorbing solution filled in the tubes. We show that light absorption by the brain should result from the combination of blood and blood-free tissue background.

Apparent dynamic contact angle of an advancing gas--liquid meniscus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kalliadasis, S.; Chang, H.

1994-01-01

The steady motion of an advancing meniscus in a gas-filled capillary tube involves a delicate balance of capillary, viscous, and intermolecular forces. The limit of small capillary numbers Ca (dimensionless speeds) is analyzed here with a matched asymptotic analysis that links the outer capillary region to the precursor film in front of the meniscus through a lubricating film. The meniscus shape in the outer region is constructed and the apparent dynamic contact angle [Theta] that the meniscus forms with the solid surface is derived as a function of the capillary number, the capillary radius, and the Hamaker's constant for intermolecularmore » forces, under conditions of weak gas--solid interaction, which lead to fast spreading of the precursor film and weak intermolecular forces relative to viscous forces within the lubricating film. The dependence on intermolecular forces is very weak and the contact angle expression has a tight upper bound tan [Theta]=7.48 Ca[sup 1/3] for thick films, which is independent of the Hamaker constant. This upper bound is in very good agreement with existing experimental data for wetting fluids in any capillary and for partially wetting fluids in a prewetted capillary. Significant correction to the Ca[sup 1/3] dependence occurs only at very low Ca, where the intermolecular forces become more important and tan [Theta] diverges slightly from the above asymptotic behavior toward lower values.« less

Advances in Spacecraft Brine Water Recovery: Development of a Radial Vaned Capillary Drying Tray

NASA Technical Reports Server (NTRS)

Callahan, Michael R.; Sargusingh, Miriam J.; Pickering, Karen D.; Weislogel, Mark M.

2014-01-01

Technology improvements in the recovery of water from brine are critical to establishing closed-loop water recovery systems, enabling long-duration missions, and achieving a sustained human presence in space. A genre of 'in-place drying' brine water recovery concepts, collectively referred to herein as Brine Residual In-Containment, are under development. These brine water recovery concepts aim to increase the overall robustness and reliability of the brine recovery process by performing drying inside the container used for final disposal of the solid residual waste. Implementation of in-place drying techniques have been demonstrated for applications where gravity is present and phase separation occurs naturally by buoyancy-induced effects. In this work, a microgravity-compatible analogue of the gravity-driven phase separation process is considered by exploiting capillarity in the form of surface wetting, surface tension, and container geometry. The proposed design consists of a series of planar radial vanes aligned about a central slotted core. Preliminary testing of the fundamental geometry in a reduced gravity environment has shown the device to spontaneously fill and saturate rapidly, thereby creating a free surface from which evaporation and phase separation can occur similar to a terrestrial-like 'cylindrical pool' of fluid. Mathematical modeling and analysis of the design suggest predictable rates of filling and stability of fluid containment as a function of relevant system dimensions; e.g., number of vanes, vane length, width, and thickness. A description of the proposed capillary design solution is presented along with preliminary results from testing, modeling, and analysis of the system.

Advancements in Spacecraft Brine Water Recovery: Development of a Radial Vaned Capillary Drying Tray

NASA Technical Reports Server (NTRS)

Callahan, Michael R.; Sargusingh, Miriam J.; Pickerin, Karen D.; Weislogel, Mark M.

2013-01-01

Technology improvements in the recovery of water from brine are critical to establishing closedloop water recovery systems, enabling long duration missions, and achieving a sustained human presence in space. A genre of 'in-place drying' brine water recovery concepts, collectively referred to herein as Brine Residual In-Containment (BRIC), are under development which aim to increase the overall robustness and reliability of the brine recovery process by performing drying inside the container used for final disposal of the solid residual waste. Implementation of in-place drying techniques have been demonstrated for applications where gravity is present and phase separation occurs naturally by buoyancy induced effects. In this work, a microgravity compatible analogue of the gravity-driven phase separation process is considered by exploiting capillarity in the form of surface wetting, surface tension, and container geometry. The proposed design consists of a series of planar radial vanes aligned about a central slotted core. Preliminary testing of the fundamental geometry in a reduced gravity environment has shown the device to spontaneously fill and saturate rapidly creating a free surface from which evaporation and phase separation can occur similar to a 1-g like 'cylindrical pool' of fluid. Mathematical modeling and analysis of the design suggest predictable rates of filling and stability of fluid containment as a function of relevant system dimensions, e.g., number of vanes, vane length, width, and thickness. A description of the proposed capillary design solution is presented along with preliminary results from testing, modeling and analysis of the system.

A Galinstan-Filled Capillary Probe for Thermal Conductivity Measurements and Its Application to Molten Eutectic {KNO}_3-{NaNO}_3-{NO}_2 (HTS) up to 700 K

NASA Astrophysics Data System (ADS)

Le Brun, Niccolò; Markides, Christos N.

2015-11-01

The successful measurement of the thermal conductivity of molten salts is a challenging undertaking due to the electrically conducting and possibly also aggressive nature of the materials, as well as the elevated temperatures at which these data are required. For accurate and reproducible measurements, it is important to develop a suitable experimental apparatus and methodology. In this study, we explore a modified version of the transient hot-wire method, which employs a molten-metal-filled capillary in order to circumvent some of the issues encountered in previous studies. Specifically, by using a novel flexible U-shaped quartz-capillary, filled with a eutectic mixture of gallium, indium and tin, commercially known as Galinstan, we proceed to measure the thermal conductivity of molten eutectic {KNO}_3-{NaNO}_3-{NaNO}_2. The new probe is demonstrated as being able to measure the thermal conductivity of this molten salt, which is found to range from 0.48 {W}{\\cdot }{m}^{-1}{\\cdot }{K}^{-1} at 500 K to 0.47 {W}{\\cdot }{m}^{-1}{\\cdot }{K}^{-1} at close to 700 K, with an overall expanded uncertainty (95 % confidence) of 3.1 %. The quartz is found to retain its electrically insulating properties and no current leakage is detected in the sample over the investigated temperature range. The thermal conductivity data reported in the present study are also used to elucidate a partial disagreement found in the literature for this material.

Liquid Acquisition Device Design Sensitivity Study

NASA Technical Reports Server (NTRS)

VanDyke, M. K.; Hastings, L. J.

2012-01-01

In-space propulsion often necessitates the use of a capillary liquid acquisition device (LAD) to assure that gas-free liquid propellant is available to support engine restarts in microgravity. If a capillary screen-channel device is chosen, then the designer must determine the appropriate combination screen mesh and channel geometry. A screen mesh selection which results in the smallest LAD width when compared to any other screen candidate (for a constant length) is desirable; however, no best screen exists for all LAD design requirements. Flow rate, percent fill, and acceleration are the most influential drivers for determining screen widths. Increased flow rates and reduced percent fills increase the through-the-screen flow pressure losses, which drive the LAD to increased widths regardless of screen choice. Similarly, increased acceleration levels and corresponding liquid head pressures drive the screen mesh selection toward a higher bubble point (liquid retention capability). After ruling out some screens on the basis of acceleration requirements alone, candidates can be identified by examining screens with small flow-loss-to-bubble point ratios for a given condition (i.e., comparing screens at certain flow rates and fill levels). Within the same flow rate and fill level, the screen constants inertia resistance coefficient, void fraction, screen pore or opening diameter, and bubble point can become the driving forces in identifying the smaller flow-loss-to-bubble point ratios.

Investigation of Coronal Leakage of Root Fillings after Smear Layer Removal with EDTA or Er,Cr:YSGG Laser through Capillary Flow Porometry

PubMed Central

Vergauwen, Tom Edgard Maria; Michiels, Rafaël; Torbeyns, Dries; Meire, Maarten; De Moor, Roeland Jozef Gentil

2014-01-01

No studies have been performed evaluating the marginal seal of root fillings after direct exposure of root canal (RC) walls to Er,Cr:YSGG laser irradiation. Therefore, 75 root filled teeth (5 × 15–cold lateral condensation) were analyzed for through-and-through leakage (TTL) using capillary flow porometry (CFP). The cleaning protocol determined the experimental groups: (1) irrigation with NaOCl 2.5% and EDTA 17% or standard protocol (SP), (2) SP + Er,Cr:YSGG lasing (dried RC), (3) NaOCl 2.5% + Er,Cr:YSGG lasing (dried RC), (4) SP + Er,Cr:YSGG lasing (wet RC), and (5) NaOCl 2.5% + Er,Cr:YSGG lasing (wet RC). Groups 6 to 10 consisted of the same filled teeth with resected apices. Resection was performed after the first CFP measurement. CFP was used to assess minimum, mean flow, and maximum pore diameters after 48 h. Statistics were performed using nonparametric tests (P > 0.05). Additional three roots per group were submitted to SEM of the RC walls. TTL was observed in all groups without statistically significant differences between the different groups for minimum, mean, and maximum pore diameter (P > 0.05). In this study, the use of EDTA and/or Er,Cr:YSGG laser did not reduce through-and-through leakage in nonresected and resected roots. PMID:24696685

Fractal modeling of fluidic leakage through metal sealing surfaces

NASA Astrophysics Data System (ADS)

Zhang, Qiang; Chen, Xiaoqian; Huang, Yiyong; Chen, Yong

2018-04-01

This paper investigates the fluidic leak rate through metal sealing surfaces by developing fractal models for the contact process and leakage process. An improved model is established to describe the seal-contact interface of two metal rough surface. The contact model divides the deformed regions by classifying the asperities of different characteristic lengths into the elastic, elastic-plastic and plastic regimes. Using the improved contact model, the leakage channel under the contact surface is mathematically modeled based on the fractal theory. The leakage model obtains the leak rate using the fluid transport theory in porous media, considering that the pores-forming percolation channels can be treated as a combination of filled tortuous capillaries. The effects of fractal structure, surface material and gasket size on the contact process and leakage process are analyzed through numerical simulations for sealed ring gaskets.

Genotyping of alpha-thalassemia deletions using multiplex polymerase chain reactions and gold nanoparticle-filled capillary electrophoresis.

PubMed

Chen, Yen-Ling; Shih, Chi-Jen; Ferrance, Jerome; Chang, Ya-Sian; Chang, Jan-Gowth; Wu, Shou-Mei

2009-02-13

A gold nanoparticle-filled capillary electrophoresis method combined with three multiplex polymerase chain reactions (PCRs) was established for simultaneous diagnosis of five common alpha-thalassemia deletions, including the -alpha(3.7) deletion, -alpha(4.2) deletion, Southeast Asian (--(SEA)), Filipino (--(FIL)) and Thai (--(THAI)) deletions. Gold nanoparticles (GNPs) were used as a pseudostationary phase to improve the resolution between DNA fragments in a low-viscosity polymer. To achieve the best CE separation, several parameters were evaluated for optimizing the separation conditions, including the capillary coating, the concentrations of polymer sieving matrix, the sizes and concentrations of GNPs, the buffer concentrations, and the pH. The final CE method for separating a 200-base pair (bp) DNA ladder and alpha-thalassemia deletions used a DB-17 capillary, 0.6% poly(ethylene oxide) (PEO) prepared in a mixture of GNP(32nm) solution and glycine buffer (25mM, pH 9.0) (80:20, v/v) as the sieving matrix with 1microM YO-PRO-1 for fluorescence detection; the applied voltage was -10kV (detector at anode side) and the separation temperature was 25 degrees C. Under these optimal conditions, 15 DNA fragments with sizes ranging from 0.2kb to 3.0kb were resolved within 11.5min. The RSDs of migration times were less than 2.81%. A total of 21 patients with alpha-thalassemia deletions were analyzed using this method, and all results showed good agreement with those obtained by gel electrophoresis.

Fully packed capillary electrochromatographic microchip with self-assembly colloidal silica beads.

PubMed

Park, Jongman; Lee, Dami; Kim, Won; Horiike, Shigeyoshi; Nishimoto, Takahiro; Lee, Se Hwan; Ahn, Chong H

2007-04-15

A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.

Quantification in an Introductory Diffusion Experiment.

ERIC Educational Resources Information Center

Snow, George E.

1991-01-01

Describes a take-home experiment in which students measure the diffusion of acid through acid-filled capillary tubes immersed into base solutions and vice versa. Students represent and analyze the effects of ambient temperature, molecular weight, and concentrations of the solutions on that movement. (MDH)

Capillary Flow in Containers of Polygonal Section: Theory and Experiment

NASA Technical Reports Server (NTRS)

Weislogel, Mark M.; Rame, Enrique (Technical Monitor)

2001-01-01

An improved understanding of the large-length-scale capillary flows arising in a low-gravity environment is critical to that engineering community concerned with the design and analysis of spacecraft fluids management systems. Because a significant portion of liquid behavior in spacecraft is capillary dominated it is natural to consider designs that best exploit the spontaneous character of such flows. In the present work, a recently verified asymptotic analysis is extended to approximate spontaneous capillary flows in a large class of cylindrical containers of irregular polygonal section experiencing a step reduction in gravitational acceleration. Drop tower tests are conducted using partially-filled irregular triangular containers for comparison with the theoretical predictions. The degree to which the experimental data agree with the theory is a testament to the robustness of the basic analytical assumption of predominantly parallel flow. As a result, the closed form analytical expressions presented serve as simple, accurate tools for predicting bulk flow characteristics essential to practical low-g system design and analysis. Equations for predicting corner wetting rates, total container flow rates, and transient surfaces shapes are provided that are relevant also to terrestrial applications such as capillary flow in porous media.

Conceptual design of a pre-loaded liquid lithium divertor target for NSTX-U

DOE PAGES

Rindt, P.; Lopes Cardozo, N. J.; van Dommelen, J. A. W.; ...

2016-09-03

In this study, a conceptual design for a pre-filled liquid lithium divertor target for the National Spherical Torus Experiment Upgrade (NSTX-U) is presented. The design is aimed at facilitating experiments with high lithium flux from the plasma facing components (PFCs) in NSTX-U and investigating the potential of capillary based liquid lithium components. In the design, lithium is supplied from a reservoir in the PFC to the plasma facing surface via capillary action in a wicking structure. This working principle is also demonstrated experimentally. Next, a titanium zirconium molybdenum (TZM) prototype design is presented, required to withstand a steady state heatmore » flux peaking at 10 MW m –2 for 5 s and edge localized modes depositing (130 kJ in 2 ms at 10 Hz). The main challenge is to sufficiently reduce the thermal stresses. This is achieved by dividing the surface into brushes and filling the slots in between with liquid lithium. The principle of using this liquid “interlayer” allows for thermal expansion and simultaneously heat conduction, and could be used to significantly reduce the demands to solids in future PFCs. Lithium flow to the surface is analyzed using a novel analytical model, ideally suited for design purposes. Thermal stresses in the PFC are analyzed using the finite element method. As a result, the requirements are met, and thus a prototype will be manufactured for physical testing.« less

Conceptual design of a pre-loaded liquid lithium divertor target for NSTX-U

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rindt, P.; Lopes Cardozo, N. J.; van Dommelen, J. A. W.

In this study, a conceptual design for a pre-filled liquid lithium divertor target for the National Spherical Torus Experiment Upgrade (NSTX-U) is presented. The design is aimed at facilitating experiments with high lithium flux from the plasma facing components (PFCs) in NSTX-U and investigating the potential of capillary based liquid lithium components. In the design, lithium is supplied from a reservoir in the PFC to the plasma facing surface via capillary action in a wicking structure. This working principle is also demonstrated experimentally. Next, a titanium zirconium molybdenum (TZM) prototype design is presented, required to withstand a steady state heatmore » flux peaking at 10 MW m –2 for 5 s and edge localized modes depositing (130 kJ in 2 ms at 10 Hz). The main challenge is to sufficiently reduce the thermal stresses. This is achieved by dividing the surface into brushes and filling the slots in between with liquid lithium. The principle of using this liquid “interlayer” allows for thermal expansion and simultaneously heat conduction, and could be used to significantly reduce the demands to solids in future PFCs. Lithium flow to the surface is analyzed using a novel analytical model, ideally suited for design purposes. Thermal stresses in the PFC are analyzed using the finite element method. As a result, the requirements are met, and thus a prototype will be manufactured for physical testing.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewers, Thomas; Heath, Jason E.; Leigh, Christi D.

The nature of geologic disposal of nuclear waste in salt formations requires validated and verified two-phase flow models of transport of brine and gas through intact, damaged, and consolidating crushed salt. Such models exist in other realms of subsurface engineering for other lithologic classes (oil and gas, carbon sequestration etc. for clastics and carbonates) but have never been experimentally validated and parameterized for salt repository scenarios or performance assessment. Models for waste release scenarios in salt back-fill require phenomenological expressions for capillary pressure and relative permeability that are expected to change with degree of consolidation, and require experimental measurement tomore » parameterize and validate. This report describes a preliminary assessment of the influence of consolidation (i.e. volume strain or porosity) on capillary entry pressure in two phase systems using mercury injection capillary pressure (MICP). This is to both determine the potential usefulness of the mercury intrusion porosimetry method, but also to enable a better experimental design for these tests. Salt consolidation experiments are performed using novel titanium oedometers, or uniaxial compression cells often used in soil mechanics, using sieved run-of-mine salt from the Waste Isolation Pilot Plant (WIPP) as starting material. Twelve tests are performed with various starting amounts of brine pore saturation, with axial stresses up to 6.2 MPa (~900 psi) and temperatures to 90°C. This corresponds to UFD Work Package 15SN08180211 milestone “FY:15 Transport Properties of Run-of-Mine Salt Backfill – Unconsolidated to Consolidated”. Samples exposed to uniaxial compression undergo time-dependent consolidation, or creep, to various degrees. Creep volume strain-time relations obey simple log-time behavior through the range of porosities (~50 to 2% as measured); creep strain rate increases with temperature and applied stress as expected. Mercury porosimetry is used to determine characteristic capillary pressure curves from a series of consolidation tests and show characteristic saturation-capillary pressure curves that follow the common van Genuchten (1978, 1980) formulation at low stresses. Higher capillary pressure data are suspect due to the large potential for sample damage, including fluid inclusion decrepitation and pore collapse. Data are supportive of use of the Leverett “J” function (Leverett, 1941) to use for scaling characteristic curves at different degrees of consolidation, but better permeability determinations are needed to support this hypothesis. Recommendations for further and refined testing are made with the goal of developing a self- consistent set of constitutive laws for granular salt consolidation and multiphase (brine-air) flow.« less

Progression of Diabetic Capillary Occlusion: A Model

PubMed Central

Gens, John Scott; Glazier, James A.; Burns, Stephen A.; Gast, Thomas J.

2016-01-01

An explanatory computational model is developed of the contiguous areas of retinal capillary loss which play a large role in diabetic maculapathy and diabetic retinal neovascularization. Strictly random leukocyte mediated capillary occlusion cannot explain the occurrence of large contiguous areas of retinal ischemia. Therefore occlusion of an individual capillary must increase the probability of occlusion of surrounding capillaries. A retinal perifoveal vascular sector as well as a peripheral retinal capillary network and a deleted hexagonal capillary network are modelled using Compucell3D. The perifoveal modelling produces a pattern of spreading capillary loss with associated macular edema. In the peripheral network, spreading ischemia results from the progressive loss of the ladder capillaries which connect peripheral arterioles and venules. System blood flow was elevated in the macular model before a later reduction in flow in cases with progression of capillary occlusions. Simulations differing only in initial vascular network structures but with identical dynamics for oxygen, growth factors and vascular occlusions, replicate key clinical observations of ischemia and macular edema in the posterior pole and ischemia in the retinal periphery. The simulation results also seem consistent with quantitative data on macular blood flow and qualitative data on venous oxygenation. One computational model applied to distinct capillary networks in different retinal regions yielded results comparable to clinical observations in those regions. PMID:27300722

Pore-scale modeling of Capillary Penetration of Wetting Liquid into 3D Fibrous Media: A Critical Examination of Equivalent Capillary Concept

NASA Astrophysics Data System (ADS)

Palakurthi, Nikhil Kumar; Ghia, Urmila; Comer, Ken

2013-11-01

Capillary penetration of liquid through fibrous porous media is important in many applications such as printing, drug delivery patches, sanitary wipes, and performance fabrics. Historically, capillary transport (with a distinct liquid propagating front) in porous media is modeled using capillary-bundle theory. However, it is not clear if the capillary model (Washburn equation) describes the fluid transport in porous media accurately, as it assumes uniformity of pore sizes in the porous medium. The present work investigates the limitations of the applicability of the capillary model by studying liquid penetration through virtual fibrous media with uniform and non-uniform pore-sizes. For the non-uniform-pore fibrous medium, the effective capillary radius of the fibrous medium was estimated from the pore-size distribution curve. Liquid penetration into the 3D virtual fibrous medium at micro-scale was simulated using OpenFOAM, and the numerical results were compared with the Washburn-equation capillary-model predictions. Preliminary results show that the Washburn equation over-predicts the height rise in the early stages (purely inertial and visco-inertial stages) of capillary transport.

Capillarity-induced disassembly of virions in carbon nanotubes

NASA Astrophysics Data System (ADS)

Fan, Xiaobin; Barclay, J. Elaine; Peng, Wenchao; Li, Yang; Li, Xianyu; Zhang, Guoliang; Evans, David J.; Zhang, Fengbao

2008-04-01

Studying the transport and fate of viruses through nanochannels is of great importance. By using the nanochannel of a carbon nanotube (CNT) as an ideal model, we evaluated the possibility of capillarity-induced viral transport through a closely fitting nanochannel and explored the mechanisms involved. It is shown both experimentally and theoretically that Cowpea mosaic virus can enter CNTs by capillarity. However, when introduced into a nanotube the protein capsid may disassemble. During the initial capillary filling stage, anomalous needle-shaped high pressure exists in the centre of the nanotube's entrance. This high pressure, combining with the significant negative pressure within the nanotube, may account for the disassembly of the virions.

Microbial growth and transport in saturated and unsaturated porous media

NASA Astrophysics Data System (ADS)

Hron, Pavel; Jost, Daniel; Bastian, Peter; Ippisch, Olaf

2014-05-01

There is a considerable ongoing effort aimed at understanding the behavior of microorganisms in porous media. Microbial activity is of significant interest in various environmental applications such as in situ bioremediation, protection of drinking water supplies and for subsurface geochemistry in general. The main limiting factors for bacterial growth are the availability of electron acceptors, nutrients and bio-available water. The capillary fringe, defined - in a wider sense than usual - as the region of the subsurface above the groundwater table, but still dominated by capillary rise, is a region where all these factors are abundantly available. It is thus a region where high microbial activity is to be expected. In a research unit 'Dynamic Capillary Fringes - A Multidisciplinary Approach (DyCap)' founded by the German Research Foundation (DFG), the growth of microorganisms in the capillary fringe was studied experimentally and with numerical simulations. Processes like component transport and diffusion, exchange between the liquid phase and the gas phase, microbial growth and cell attachment and detachment were incorporated into a numerical simulator. The growth of the facultative anaerobic Escherichia coli as a function of nutrient availability and oxygen concentration in the liquid phase is modeled with modified Monod-type models and modifications for the switch between aerobic and anaerobic growth. Laboratory batch experiments with aqueous solutions of bacteria have been carried out under various combinations of oxygen concentrations in the gas phase and added amounts of dissolved organic carbon to determine the growth model parameters by solution of a parameter estimation problem. For the transport of bacteria the adhesion to phase boundaries is also very important. As microorganisms are transported through porous media, they are removed from the pore fluid by physicochemical filtration (attachment to sediment grain surfaces) or are adhering to gas-water interface. The cell attachment and detachment model was based on flow-through experiments and the parameters were obtained by fitting the model to measured bacteria breakthrough curves. Experiments on bacterial growth in porous media with and without groundwater flow were performed in Hele-Shaw cells filled with quartz sands. The cell density was determined by the fluorescence of a special protein produced by the genetically modified strain of E. coli. The simulation results are compared to experimental data and different modeling approaches are discussed.

Capillary flow weld-bonding

NASA Technical Reports Server (NTRS)

Vaughan, R. W.; Jones, R. J. (Inventor)

1976-01-01

The invention of a weld-bonding technique for titanium plates was described. This involves fastening at least two plates of titanium together using spot-welding and applying a bead of adhesive along the edge of the resistance spot-welded joint which upon heating, flows and fills the separation between the joint components.

An experimental investigation of bubble splitting through multiple bifurcations

NASA Astrophysics Data System (ADS)

Bull, Joseph L.; Eshpuniyani, Brijesh; Fowlkes, J. Brian

2004-11-01

A bench top vascular bifurcation model is used to investigate the splitting of long bubbles in a series of liquid-filled bifurcations. These experiments are motivated by a gas embolotherapy technique for the potential treatment of cancer by using gas emboli to infarct tumors. The gas bubbles originate as perfluorocarbon droplets that are small enough to pass through capillaries and are injected into the bloodstream. Low intensity ultrasound is used to track their motion, and they are vaporized at the desired location for treatment via high intensity ultrasound to produce gas bubbles whose volumes are approximately 125 to 150 times the droplet volume. Achieving complete tumor necrosis requires infarction of most of the tumor. Understanding the transport and splitting of the gas bubbles, which can be long enough to extend through more than one bifurcation, is necessary to design delivery strategies. The current experiments investigate the behavior of a bubble as it passes through a series of two geometrically symmetric bifurcations, for different values of effective Bond number, which depends on gravity and the positioning of the bifurcation, capillary number, and bubble volume. The experiments are designed to match the Reynolds, Bond and capillary numbers to the physiological values for arterioles, and to provide guidance in achieving uniform tumor infarction. This work is supported by NSF grant BES-0301278 and NIH grant EB003541-01.

Effects of aperture variability and wettability on immiscible displacement in fractures

NASA Astrophysics Data System (ADS)

Yang, Zhibing; Méheust, Yves; Neuweiler, Insa

2017-04-01

Fluid-fluid displacement in porous and fractured media is an important process. Understanding and controlling this process is key to many practical applications, such as hydrocarbon recovery, geological storage of CO2, groundwater remediation, etc. Here, we numerically study fluid-fluid displacement in rough-walled fractures. We focus on the combined effect of wettability and fracture surface topography on displacement patterns and interface growth. We develop a novel numerical model to simulate dynamic fluid invasion under the influence of capillary and viscous forces. The capillary force is calculated using the two principal curvatures (aperture-induced curvature and in-plane curvature) at the fluid-fluid interface, and the viscous force is taken into account by solving the fluid pressure distribution. The aperture field of a fracture is represented by a spatially correlated random field, which is described by a power spectrum for the fracture wall topography and a cutoff wave-length. We numerically produce displacement patterns ranging from stable displacement, capillary fingering, and viscous fingering, as well as the transitions between them. We show that both reducing the aperture variability and increasing the contact angle (from drainage to weak imbibition) stabilize the displacement due to the influence of the in-plane curvature, an effect analogous to that of the cooperative pore filling in porous media. Implications of these results will be discussed.

Automated one-step DNA sequencing based on nanoliter reaction volumes and capillary electrophoresis.

PubMed

Pang, H M; Yeung, E S

2000-08-01

An integrated system with a nano-reactor for cycle-sequencing reaction coupled to on-line purification and capillary gel electrophoresis has been demonstrated. Fifty nanoliters of reagent solution, which includes dye-labeled terminators, polymerase, BSA and template, was aspirated and mixed with the template inside the nano-reactor followed by cycle-sequencing reaction. The reaction products were then purified by a size-exclusion chromatographic column operated at 50 degrees C followed by room temperature on-line injection of the DNA fragments into a capillary for gel electrophoresis. Over 450 bases of DNA can be separated and identified. As little as 25 nl reagent solution can be used for the cycle-sequencing reaction with a slightly shorter read length. Significant savings on reagent cost is achieved because the remaining stock solution can be reused without contamination. The steps of cycle sequencing, on-line purification, injection, DNA separation, capillary regeneration, gel-filling and fluidic manipulation were performed with complete automation. This system can be readily multiplexed for high-throughput DNA sequencing or PCR analysis directly from templates or even biological materials.

Time resolved EUV spectra from Zpinching capillary discharge plasma

NASA Astrophysics Data System (ADS)

Jancarek, Alexandr; Nevrkla, Michal; Nawaz, Fahad

2015-09-01

We developed symmetrically charged driver to obtain high voltage, high current Z-pinching capillary discharge. Plasma is created by up to 70 kA, 29 ns risetime current pulse passing through a 5 mm inner diameter, 224 mm long capillary filled with gas to initial pressure in the range of 1 kPa. Due to the low inductance design of the driver, the pinch is observable directly from the measured current curve. Time-integrated and time-resolved spectra of discharge plasma radiation are recorded together with the capillary current and analyzed. The most encouraging spectra were captured in the wavelength range 8.3 ÷ 14 nm. This spectral region contains nitrogen Balmer series lines including potentially lasing NVII 2 - 3 transition. Spectral lines are identified in the NIST database using the FLY kinetic code. The line of 13.38 nm wavelength, transition NVII 2 - 3, was observed in gated, and also in time-integrated spectra for currents >60 kA. This work has been supported by the Ministry of Education, Youth and Sports of the Czech Republic grants LG13029.

Mechanism of metabolic stroke and spontaneous cerebral hemorrhage in glutaric aciduria type I

PubMed Central

2014-01-01

Background Metabolic stroke is the rapid onset of lasting central neurological deficit associated with decompensation of an underlying metabolic disorder. Glutaric aciduria type I (GA1) is an inherited disorder of lysine and tryptophan metabolism presenting with metabolic stroke in infancy. The clinical presentation includes bilateral striatal necrosis and spontaneous subdural and retinal hemorrhages, which has been frequently misdiagnosed as non-accidental head trauma. The mechanisms underlying metabolic stroke and spontaneous hemorrhage are poorly understood. Results Using a mouse model of GA1, we show that metabolic stroke progresses in the opposite sequence of ischemic stroke, with initial neuronal swelling and vacuole formation leading to cerebral capillary occlusion. Focal regions of cortical followed by striatal capillaries are occluded with shunting to larger non-exchange vessels leading to early filling and dilation of deep cerebral veins. Blood–brain barrier breakdown was associated with displacement of tight-junction protein Occludin. Conclusion Together the current findings illuminate the pathophysiology of metabolic stroke and vascular compromise in GA1, which may translate to other neurometabolic disorders presenting with stroke. PMID:24468193

Mechanism of metabolic stroke and spontaneous cerebral hemorrhage in glutaric aciduria type I.

PubMed

Zinnanti, William J; Lazovic, Jelena; Housman, Cathy; Antonetti, David A; Koeller, David M; Connor, James R; Steinman, Lawrence

2014-01-27

Metabolic stroke is the rapid onset of lasting central neurological deficit associated with decompensation of an underlying metabolic disorder. Glutaric aciduria type I (GA1) is an inherited disorder of lysine and tryptophan metabolism presenting with metabolic stroke in infancy. The clinical presentation includes bilateral striatal necrosis and spontaneous subdural and retinal hemorrhages, which has been frequently misdiagnosed as non-accidental head trauma. The mechanisms underlying metabolic stroke and spontaneous hemorrhage are poorly understood. Using a mouse model of GA1, we show that metabolic stroke progresses in the opposite sequence of ischemic stroke, with initial neuronal swelling and vacuole formation leading to cerebral capillary occlusion. Focal regions of cortical followed by striatal capillaries are occluded with shunting to larger non-exchange vessels leading to early filling and dilation of deep cerebral veins. Blood-brain barrier breakdown was associated with displacement of tight-junction protein Occludin. Together the current findings illuminate the pathophysiology of metabolic stroke and vascular compromise in GA1, which may translate to other neurometabolic disorders presenting with stroke.

Design of Capillary Flows with Spatially Graded Porous Films

NASA Astrophysics Data System (ADS)

Joung, Young Soo; Figliuzzi, Bruno Michel; Buie, Cullen

2013-11-01

We have developed a new capillary tube model, consisting of multi-layered capillary tubes oriented in the direction of flow, to predict capillary speeds on spatially graded porous films. Capillary flows through thin porous media have been widely utilized for small size liquid transport systems. However, for most media it is challenging to realize arbitrary shapes and spatially functionalized micro-structures with variable flow properties. Therefore, conventional media can only be used for capillary flows obeying Washburn's equation and the modifications thereof. Given this background, we recently developed a method called breakdown anodization (BDA) to produce highly wetting porous films. The resulting surfaces show nearly zero contact angles and fast water spreading speed. Furthermore, capillary pressure and spreading diffusivity can be expressed as functions of capillary height when customized electric fields are used in BDA. From the capillary tube model, we derived a general capillary flow equation of motion in terms of capillary pressure and spreading diffusivity. The theoretical model shows good agreement with experimental capillary flows. The study will provide novel design methodologies for paper-based microfluidic devices.

Shifts in pore connectivity from precipitation versus groundwater rewetting increases soil carbon loss after drought

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, A. Peyton; Bond-Lamberty, Ben; Benscoter, Brian W.

Droughts and other extreme precipitation events are predicted to increase in intensity, duration and extent, with uncertain implications for terrestrial carbon (C) sequestration. Soil wetting from above (precipitation) results in a characteristically different pattern of pore-filling than wetting from below (groundwater), with larger, well-connected pores filling before finer pore spaces, unlike groundwater rise in which capillary forces saturate the finest pores first. Here we demonstrate that pore-scale wetting patterns interact with antecedent soil moisture conditions to alter pore-, core- and field-scale C dynamics. Drought legacy and wetting direction are perhaps more important determinants of short-term C mineralization than current soilmore » moisture content in these soils. Our results highlight that microbial access to C is not solely limited by physical protection, but also by drought or wetting-induced shifts in hydrologic connectivity. We argue that models should treat soil moisture within a three-dimensional framework emphasizing hydrologic conduits for C and resource diffusion.« less

Comparison of influence of incorporated 3d-, 4d- and 4f-metal chlorides on electronic properties of single-walled carbon nanotubes

NASA Astrophysics Data System (ADS)

Kharlamova, M. V.

2013-06-01

In the present work, the channels of single-walled carbon nanotubes were filled with melts of ZnCl2, CdCl2, and TbCl3 by a capillary method with subsequent slow cooling. The detailed study of electronic structure of filled nanotubes was performed using Raman, optical absorption, and X-ray photoelectron spectroscopy. The obtained data are in mutual agreement and it proves that the filling of carbon nanotube channels with all these salts leads to the charge transfer from nanotube walls to the incorporated compounds, thus acceptor doping of nanotubes takes place. It was found out that encapsulated terbium chloride has the largest influence on the electronic properties of carbon nanotubes.

Automatic cryogenic liquid level controller is safe for use near combustible substances

NASA Technical Reports Server (NTRS)

Krejsa, M.

1966-01-01

Automatic mechanical liquid level controller that is independent of any external power sources is used with safety in the presence of combustibles. A gas filled capillary tube which leads from a pressurized chamber, is inserted into the cryogenic liquid reservoir and becomes a liquid level sensing element or probe.

Capillary Rise: Validity of the Dynamic Contact Angle Models.

PubMed

Wu, Pingkeng; Nikolov, Alex D; Wasan, Darsh T

2017-08-15

The classical Lucas-Washburn-Rideal (LWR) equation, using the equilibrium contact angle, predicts a faster capillary rise process than experiments in many cases. The major contributor to the faster prediction is believed to be the velocity dependent dynamic contact angle. In this work, we investigated the dynamic contact angle models for their ability to correct the dynamic contact angle effect in the capillary rise process. We conducted capillary rise experiments of various wetting liquids in borosilicate glass capillaries and compared the model predictions with our experimental data. The results show that the LWR equations modified by the molecular kinetic theory and hydrodynamic model provide good predictions on the capillary rise of all the testing liquids with fitting parameters, while the one modified by Joos' empirical equation works for specific liquids, such as silicone oils. The LWR equation modified by molecular self-layering model predicts well the capillary rise of carbon tetrachloride, octamethylcyclotetrasiloxane, and n-alkanes with the molecular diameter or measured solvation force data. The molecular self-layering model modified LWR equation also has good predictions on the capillary rise of silicone oils covering a wide range of bulk viscosities with the same key parameter W(0), which results from the molecular self-layering. The advantage of the molecular self-layering model over the other models reveals the importance of the layered molecularly thin wetting film ahead of the main meniscus in the energy dissipation associated with dynamic contact angle. The analysis of the capillary rise of silicone oils with a wide range of bulk viscosities provides new insights into the capillary dynamics of polymer melts.

On Thermocapillary Mechanism of Spatial Separation of Metal Melts

NASA Astrophysics Data System (ADS)

Demin, V. A.; Mizev, A. I.; Petukhov, M. I.

2018-02-01

Theoretical research has been devoted to the study of binary metal melts behavior in a thin capillary. Earlier it has been found experimentally that unusually significant and quick redistribution of melts components takes place along capillary after the cooling. Numerical simulation of concentration-induced convection has been carried out to explain these experimental data. Two-component melt of both liquid metals filling vertical thin capillary with non-uniform temperature distribution on the boundaries is considered. It is assumed that the condition of absolute non-wetting is valid on the sidewalls. Because of this effect there is a free surface on vertical boundaries, where thermocapillary force is appeared due to the external longitudinal temperature gradient. It makes to move liquid elements at a big distance, compared with axial size of capillary. Effects of adsorption-desorption on the surface, thermal and concentration-capillary forces, convective motion in a volume and diffusion generate the large-scale circulation. This process includes the admixture carrying-out on the surface in the more hot higher part of the channel, its following transfer down along the boundary due to the thermocapillary force and its return in the volume over the desorption in the lower part of capillary. Intensity of motion and processes of adsorption-desorption on the free boundary have the decisive influence upon the formation of concentration fields and speed of components redistribution. Thus, one of the possible mechanisms of longitudinal division on components of liquid binary mixtures in thin channels has been demonstrated.

Rare-earth metal halogenide encapsulation-induced modifications in Raman spectra of single-walled carbon nanotubes

NASA Astrophysics Data System (ADS)

Kharlamova, M. V.

2015-01-01

In the present work, a detailed Raman spectroscopy investigation on the single-walled carbon nanotubes (SWCNTs) filled with praseodymium chloride, terbium chloride and thulium chloride was performed. The salts were incorporated inside the SWCNTs by a capillary filling method using melts, and the high-resolution transmission electron microscopy data proved the high filling degree of the nanotube channels. A thorough analysis of the radial breathing mode and G-band of the Raman spectra of the pristine and filled SWCNTs showed that the encapsulated salts cause acceptor doping of the host nanotubes, and the doping efficiency depends on the compound. The incorporated thulium chloride has the strongest doping effect on the SWCNTs, whereas praseodymium chloride has the weakest effect. It was found that the encapsulated salts modify more significantly the electronic structure of metallic nanotubes than semiconducting SWCNTs.

Filling carbon nanotubes with particles.

PubMed

Kim, Byong M; Qian, Shizhi; Bau, Haim H

2005-05-01

The filling of carbon nanotubes (CNTs) with fluorescent particles was studied experimentally and theoretically. The fluorescent signals emitted by the particles were visible through the walls of the nanotubes, and the particles inside the tubes were observable with an electron microscope. Taking advantage of the template-grown carbon nanotubes' transparency to fluorescent light, we measured the filling rate of the tubes with particles at room conditions. Liquids such as ethylene glycol, water, and ethylene glycol/water mixtures, laden with 50 nm diameter fluorescent particles, were brought into contact with 500 nm diameter CNTs. The liquid and the particles' transport were observed, respectively, with optical and fluorescence microscopy. The CNTs were filled controllably with particles by the complementary action of capillary forces and the evaporation of the liquid. The experimental results were compared and favorably agreed with theoretical predictions. This is the first report on fluorescence studies of particle transport in carbon nanotubes.

A model for capillary rise in micro-tube restrained by a sticky layer

NASA Astrophysics Data System (ADS)

Shen, Anqi; Xu, Yun; Liu, Yikun; Cai, Bo; Liang, Shuang; Wang, Fengjiao

2018-06-01

Fluid transport in a microscopic capillary under the effects of a sticky layer was theoretically investigated. A model based on the classical Lucas-Washburn (LW) model is proposed for the meniscus rise with the sticky layer present. The sticky layer consists of two parts: a fixed (located at the wall) and a movable part (located on the inside of the capillary), affecting the micro-capillary flow in different ways. Within our model, the movable layer is defined by the capillary radius and pressure gradient. From the model it follows that the fixed sticky layer leads to a decrease of capillary radius, while the movable sticky layer increases flow resistance. The movable layer thickness varies with the pressure gradient, which in turn varies with the rising of the meniscus. The results of our theoretical calculation also prove that the capillary radius has a greater effect on the meniscus height, rather than the additional resistance caused by the movable layer. Moreover, the fixed sticky layer, which affects the capillary radius, has a greater influence than the movable sticky layer. We conclude that the sticky layer causes a lower imbibition height than the LW model predicts.

The effect of concentration in the patterning of silica particles by the soft lithographic technique

NASA Astrophysics Data System (ADS)

Singh, Akanksha; Malek, Chantal Khan; Kulkarni, Sulabha K.

2008-12-01

Soft lithography provides remarkable surface patterning techniques to organize colloidal particles for a wide variety of applications. In particular, micromolding in capillaries (MIMIC) has emerged as a patterning method in the nanometer to micrometer scale in a single step by using templating and directing nanoparticles via capillary forces in the channel. The present work reports the results of the micropatterning of monodispersed silica particles of ~338 ± 2 nm size in ethanol medium, using MIMIC on silicon substrates. The effect of the concentration of silica particles on the patterning has been investigated. The patterns are well aligned and completely filled at 2 wt% concentration of silica particles.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewers, Thomas; Heath, Jason E.; Leigh, Christi D.

The nature of geologic disposal of nuclear waste in salt formations requires validated and verified two - phase flow models of transport of brine and gas through intact, damaged, and consolidating crushed salt. Such models exist in oth er realms of subsurface engineering for other lithologic classes (oil and gas, carbon sequestration etc. for clastics and carbonates) but have never been experimentally validated and parameterized for salt repository scenarios or performance assessment. Mo dels for waste release scenarios in salt back - fill require phenomenological expressions for capillary pressure and relative permeability that are expected to change with degree ofmore » consolidation, and require experimental measurement to parameterize and vali date. This report describes a preliminary assessment of the influence of consolidation (i.e. volume strain or porosity) on capillary entry pressure in two phase systems using mercury injection capillary pressure (MICP). This is to both determine the potent ial usefulness of the mercury intrusion porosimetry method, but also to enable a better experimental design for these tests. Salt consolidation experiments are performed using novel titanium oedometers, or uniaxial compression cells often used in soil mech anics, using sieved run - of - mine salt from the Waste Isolation Pilot Plant (WIPP) as starting material. Twelve tests are performed with various starting amounts of brine pore saturation, with axial stresses up to 6.2 MPa (%7E900 psi) and temperatures to 90 o C. This corresponds to UFD Work Package 15SN08180211 milestone "FY:15 Transport Properties of Run - of - Mine Salt Backfill - Unconsolidated to Consolidated". Samples exposed to uniaxial compression undergo time - dependent consolidation, or creep, to various deg rees. Creep volume strain - time relations obey simple log - time behavior through the range of porosities (%7E50 to 2% as measured); creep strain rate increases with temperature and applied stress as expected. Mercury porosimetry is used to determine characteri stic capillary pressure curves from a series of consolidation tests and show characteristic saturation - capillary pressure curves that follow the common van Genuchten (1978, 1980) formulation at low stresses. Higher capillary pressure data are suspect due t o the large potential for sample damage, including fluid inclusion decrepitation and pore collapse. Data are supportive of use of the Leverett "J" function (Leverett, 1941) to use for scaling characteristic curves at different degrees of consolidation, but better permeability determinations are needed to support this hypothesis. Recommendations for further and refined testing are made with the goal of developing a self - consistent set of constitutive laws for granular salt consolidation and multiphase (brin e - air) flow.« less

Integrated microfluidic chip for rapid DNA digestion and time-resolved capillary electrophoresis analysis

PubMed Central

Lin, Che-Hsin; Wang, Yao-Nan; Fu, Lung-Ming

2012-01-01

An integrated microfluidic chip is proposed for rapid DNA digestion and time-resolved capillary electrophoresis (CE) analysis. The chip comprises two gel-filled chambers for DNA enrichment and purification, respectively, a T-form micromixer for DNA/restriction enzyme mixing, a serpentine channel for DNA digestion reaction, and a CE channel for on-line capillary electrophoresis analysis. The DNA and restriction enzyme are mixed electroomostically using a pinched-switching DC field. The experimental and numerical results show that a mixing performance of 97% is achieved within a distance of 1 mm from the T-junction when a driving voltage of 90 V/cm and a switching frequency of 4 Hz are applied. Successive mixing digestion and capillary electrophoresis operation clearly present the changes on digesting φx-174 DNA in different CE runs. The time-resolved electropherograms show that the proposed device enables a φx-174 DNA sample comprising 11 fragments to be concentrated and analyzed within 24 min. Overall, the results presented in this study show that the proposed microfluidic chip provides a rapid and effective tool for DNA digestion and CE analysis applications. PMID:22662085

Influence of steric hindrance on enantioseparation of Dns-amino acids and pesticides on terguride based chiral selectors in capillary electrophoresis.

PubMed

Honzátko, Ales; Cvak, Jan; Vaingátová, Silvie; Flieger, Miroslav

2005-05-01

Three urea derivatives of ergoline-based chiral selectors (CSs), differing in the size of the urea side chain, i.e. dimethyl- (CSI), diethyl- (CSII), and diisopropylurea (CSIII), were used to study the effect of steric hindrance on the enantioseparation of dansyl amino acids (Dns-AAs), pesticides, and mandelic acid under condition of capillary electrophoresis (CE) in linear polyacrylamide coated capillaries. A mixture of organic modifiers (MeOH/THF, 4:1 v/v) in a BGE consisting of 100 mM beta-alanine-acetate was used to increase the solubility of CSs up to 25 mM. The capillary was filled with CS (high UV absorption), and the inlet and outlet vials contained buffer solutions only. The best enantioseparation of Dns-AAs was achieved on CSI. Increased steric hindrance of the chiral binding site led to reduction of both enantioselectivity and resolution. The opposite pattern was observed for the separation of mandelic acid enantiomers, where the best enantioseparation and resolution was obtained with CSIII. Most of the pesticides studied reached maximum selectivity on the diethylurea ergoline derivative (CSII). Enantioseparation of fenoxaprop was found to be independent of steric hindrance.

Analysis of Cement-Based Pastes Mixed with Waste Tire Rubber

NASA Astrophysics Data System (ADS)

Sola, O. C.; Ozyazgan, C.; Sayin, B.

2017-03-01

Using the methods of thermal gravimetry, differential thermal analysis, Furier transform infrared analysis, and capillary absorption, the properties of a cement composite produced by introducing waste tyre rubber into a cement mixture were investigated. It was found that the composite filled with the rubber had a much lower water absorption ability than the unfilled one.

Liquid-Vapor Coexistence at a Mesoporous Substrate

NASA Astrophysics Data System (ADS)

Kityk, A. V.; Hofmann, T.; Knorr, K.

2008-01-01

The condensation of hexane vapor onto a mesoporous Si substrate with a pore radius of 3.5 nm has been studied by means of volumetry and ellipsometry. The filling fraction of the pores and the coverage of the substrate have been determined. The coverage of the regime after the completion of capillary condensation has been compared to recent theoretical work.

Improvement of chromatographic performances of in-situ synthesized hybrid C8 silica monoliths by reduction of structural radial heterogeneities.

PubMed

Roux, R; Abi Jaoudé, M; Demesmay, C

2009-05-01

Several modifications of a previously described protocol are proposed to improve the performances of in-situ synthesized C(8) hybrid silica monoliths. Our attention was focused on reducing the sources of radial heterogeneity that may be responsible for the poor efficiencies observed in the hydrodynamic elution mode. It was demonstrated that a decrease in the temperature of the capillary during the filling step equally to that of the polymerization mixture (0 degrees C), associated with a decrease of the gelation temperature to 20 degrees C along with a new pre-treatment of the capillary's internal walls [with a mixture of tetraethoxysilane (TEOS)/EtOH (1/3, v/v)] allows (i) increasing the radial homogeneity of the monolith, thus further enhancing the performances in the nano-liquid chromatography (nano-LC) mode, (ii) improving the capillary to capillary reproducibility in terms of permeability and efficiencies. In fact, the average minimum plate height H(min) was lowered from 24 to 14 microm and the capillary-to-capillary reproducibility of the synthesis was widely improved by factors two and three of reduction on the calculated standard deviation, respectively for both the efficiency in the nano-LC mode and the permeability. At last, the improved radial homogeneity and anchoring of the synthesized monoliths allowed increasing the inner diameter of the capillary (up to 150 microm) without any significant loss in efficiency. Finally, long term stability of the as-obtained monolithic stationary phases in terms of retention and efficiency was studied. In addition, the evaluation of their chromatographic behaviour was also achieved with the Tanaka test and the results were compared to those already published for commercial monoliths (Chromolith) as well as for particulate stationary phases.

Nonlinear model and attitude dynamics of flexible spacecraft with large amplitude slosh

NASA Astrophysics Data System (ADS)

Deng, Mingle; Yue, Baozeng

2017-04-01

This paper is focused on the nonlinearly modelling and attitude dynamics of spacecraft coupled with large amplitude liquid sloshing dynamics and flexible appendage vibration. The large amplitude fuel slosh dynamics is included by using an improved moving pulsating ball model. The moving pulsating ball model is an equivalent mechanical model that is capable of imitating the whole liquid reorientation process. A modification is introduced in the capillary force computation in order to more precisely estimate the settling location of liquid in microgravity or zero-g environment. The flexible appendage is modelled as a three dimensional Bernoulli-Euler beam and the assumed modal method is employed to derive the nonlinear mechanical model for the overall coupled system of liquid filled spacecraft with appendage. The attitude maneuver is implemented by the momentum transfer technique, and a feedback controller is designed. The simulation results show that the liquid sloshing can always result in nutation behavior, but the effect of flexible deformation of appendage depends on the amplitude and direction of attitude maneuver performed by spacecraft. Moreover, it is found that the liquid sloshing and the vibration of flexible appendage are coupled with each other, and the coupling becomes more significant with more rapid motion of spacecraft. This study reveals that the appendage's flexibility has influence on the liquid's location and settling time in microgravity. The presented nonlinear system model can provide an important reference for the overall design of the modern spacecraft composed of rigid platform, liquid filled tank and flexible appendage.

Adsorption and movement of water by skin of the Australian thorny devil (Agamidae: Moloch horridus)

NASA Astrophysics Data System (ADS)

Comanns, Philipp; Esser, Falk J.; Kappel, Peter H.; Baumgartner, Werner; Shaw, Jeremy; Withers, Philip C.

2017-09-01

Moisture-harvesting lizards, such as the Australian thorny devil Moloch horridus, have remarkable adaptations for inhabiting arid regions. Their microstructured skin surface, with channels in between overlapping scales, enables them to collect water by capillarity and passively transport it to the mouth for ingestion. We characterized this capillary water transport for live thorny devils using high-speed video analyses. Comparison with preserved specimens showed that live lizards are required for detailed studies of skin water transport. For thorny devils, there was no directionality in cutaneous water transport (unlike Phrynosoma) as 7 µl water droplets applied to the skin were transported radially over more than 9.2 mm. We calculated the total capillary volume as 5.76 µl cm-2 (dorsal) and 4.45 µl cm-2 (ventral), which is reduced to 50% filling by the time transportation ceases. Using micro-computed tomography and scanning electron microscopy of shed skin to investigate capillary morphology, we found that the channels are hierarchically structured as a large channel between the scales that is sub-divided by protrusions into smaller sub-capillaries. The large channel quickly absorbs water whereas the sub-capillary structure extends the transport distance by about 39% and potentially reduces the water volume required for drinking. An adapted dynamics function, which closely reflects the channel morphology, includes that ecological role.

Adsorption and movement of water by skin of the Australian thorny devil (Agamidae: Moloch horridus).

PubMed

Comanns, Philipp; Esser, Falk J; Kappel, Peter H; Baumgartner, Werner; Shaw, Jeremy; Withers, Philip C

2017-09-01

Moisture-harvesting lizards, such as the Australian thorny devil Moloch horridus , have remarkable adaptations for inhabiting arid regions. Their microstructured skin surface, with channels in between overlapping scales, enables them to collect water by capillarity and passively transport it to the mouth for ingestion. We characterized this capillary water transport for live thorny devils using high-speed video analyses. Comparison with preserved specimens showed that live lizards are required for detailed studies of skin water transport. For thorny devils, there was no directionality in cutaneous water transport (unlike Phrynosoma ) as 7 µl water droplets applied to the skin were transported radially over more than 9.2 mm. We calculated the total capillary volume as 5.76 µl cm -2 (dorsal) and 4.45 µl cm -2 (ventral), which is reduced to 50% filling by the time transportation ceases. Using micro-computed tomography and scanning electron microscopy of shed skin to investigate capillary morphology, we found that the channels are hierarchically structured as a large channel between the scales that is sub-divided by protrusions into smaller sub-capillaries. The large channel quickly absorbs water whereas the sub-capillary structure extends the transport distance by about 39% and potentially reduces the water volume required for drinking. An adapted dynamics function, which closely reflects the channel morphology, includes that ecological role.

Preparation and evaluation of molecularly imprinted polymers based on 9-ethyladenine for the recognition of nucleotide bases in capillary electrochromatography.

PubMed

Huang, Yi-Chen; Lin, Chun-Chi; Liu, Chuen-Ying

2004-02-01

A molecularly imprinted polymer (MIP) comprising 9-ethyladenine was polymerized in situ inside the capillary for the electrochromatographic separation of nucleotide bases. The capillary wall was first functionalized with 3-trimethoxysilylpropyl methacrylate (10% v/v) and 1,1-diphenyl-2-picrylhydrazyl (0.01% w/v) in toluene. Following this treatment, the capillary was filled with acetonitrile containing 9-ethyladenine, methacrylic acid, ethylene glycol dimethacrylate, and initiator. After polymerization, the MIP was shrunk into a film against the inner wall of the capillary with the syringe pump. The template was then removed with methanol under nitrogen flow. For evaluation the feasibility of the MIP column for the separation of nucleotide bases, some parameters including the pH, concentration of the background electrolyte, the applied voltage as well as the effect of organic modifier were studied. The migration behavior of nucleotide bases on the MIP column was also compared with that on the bare fused-silica column. The results indicated that the MIP columns demonstrated better recognition properties at a pH range of 6-8. The efficiency (plates/m) at pH 8 for the nonimprinted analyte was 75,300 for cytosine, 50,200 for thymine, and 14,800 for guanine. However, the efficiency for the imprinted analyte, adenine, was quite low. This was evidenced by the broad peak, yielding only 2600 plates/m.

Shapes and dynamics of miscible liquid/liquid interfaces in horizontal capillary tubes.

PubMed

Stevar, M S P; Vorobev, A

2012-10-01

We report optical observations of the dissolution behaviour of glycerol/water, soybean oil/hexane, and isobutyric acid (IBA)/water binary mixtures within horizontal capillary tubes. Tubes with diameters as small as 0.2mm were initially filled with one component of the binary mixture (solute) and then immersed into a solvent-filled thermostatic bath. Both ends of the tubes were open, and no pressure difference was applied between the ends. In the case of glycerol/water and soybean oil/hexane mixtures, we managed to isolate the dissolution (the interfacial mass transfer) from the hydrodynamic motion. Two phase boundaries moving from the ends into the middle section of the tube with the speeds v∼D(1/3)t(-2/3)d(2) (D,t and d are the coefficient of diffusion, time and the diameter of the tube, respectively) were observed. The boundaries slowly smeared but their smearing occurred considerably slower than their motion. The motion of the phase boundaries cannot be explained by the dependency of the diffusion coefficient on concentration, and should be explained by the effect of barodiffusion. The shapes of the solute/solvent boundaries are defined by the balance between gravity and surface tension effects. The contact line moved together with the bulk interface: no visible solute remained on the walls after the interface passage. Changes in temperature and in the ratio between gravity and capillary forces altered the apparent contact angles. The IBA/water system had different behaviour. Below the critical (consolute) point, no dissolution was observed: IBA and water behaved like two immiscible liquids, with the IBA phase being displaced from the tube by capillary pressure (the spontaneous imbibition process). Above the critical point, two IBA/water interfaces could be identified, however the interfaces did not penetrate much into the tube. Copyright © 2012 Elsevier Inc. All rights reserved.

Modeling capillary bridge dynamics and crack healing between surfaces of nanoscale roughness

NASA Astrophysics Data System (ADS)

Soylemez, Emrecan; de Boer, Maarten P.

2017-12-01

Capillary bridge formation between adjacent surfaces in humid environments is a ubiquitous phenomenon. It strongly influences tribological performance with respect to adhesion, friction and wear. Only a few studies, however, assess effects due to capillary dynamics. Here we focus on how capillary bridge evolution influences crack healing rates. Experimental results indicated a logarithmic decrease in average crack healing velocity as the energy release rate increases. Our objective is to model that trend. We assume that capillary dynamics involve two mechanisms: capillary bridge growth and subsequently nucleation followed by growth. We show that by incorporating interface roughness details and the presence of an adsorbed water layer, the behavior of capillary force dynamics can be understood quantitatively. We identify three important regimes that control the healing process, namely bridge growth, combined bridge growth and nucleation, and finally bridge nucleation. To fully capture the results, however, the theoretical model for nucleation time required an empirical modification. Our model enables significant insight into capillary bridge dynamics, with a goal of attaining a predictive capability for this important microelectromechanical systems (MEMS) reliability failure mechanism.

A Zero Dimensional Time-Dependent Model of High-Pressure Ablative Capillary Discharge (Preprint)

DTIC Science & Technology

2008-06-01

comprehensive model of capillary discharge is important to understand the physics and engineering aspects of the capillary discharge thruster. A schematic...investigators since the mid-1980s see 1-11 and references therein, satisfy both of these conditions well. These studies investigated the dynamics of high...is a comprehensive description of the radiative heat transfer in the capillary discharge. It is worth noting that in other types of capillary

Finite volume solution for two-phase flow in a straight capillary

NASA Astrophysics Data System (ADS)

Yelkhovsky, Alexander; Pinczewski, W. Val

2018-04-01

The problem of two-phase flow in straight capillaries of polygonal cross section displays many of the dynamic characteristics of rapid interfacial motions associated with pore-scale displacements in porous media. Fluid inertia is known to be important in these displacements but is usually ignored in network models commonly used to predict macroscopic flow properties. This study presents a numerical model for two-phase flow which describes the spatial and temporal evolution of the interface between the fluids. The model is based on an averaged Navier-Stokes equation and is shown to be successful in predicting the complex dynamics of both capillary rise in round capillaries and imbibition along the corners of polygonal capillaries. The model can form the basis for more realistic network models which capture the effect of capillary, viscous, and inertial forces on pore-scale interfacial dynamics and consequent macroscopic flow properties.

Sol-gel approach to in situ creation of high pH-resistant surface-bonded organic-inorganic hybrid zirconia coating for capillary microextraction (in-tube SPME).

PubMed

Alhooshani, Khalid; Kim, Tae-Young; Kabir, Abuzar; Malik, Abdul

2005-01-07

A novel zirconia-based hybrid organic-inorganic sol-gel coating was developed for capillary microextraction (CME) (in-tube SPME). High degree of chemical inertness inherent in zirconia makes it very difficult to covalently bind a suitable organic ligand to its surface. In the present work, this problem was addressed from a sol-gel chemistry point of view. Principles of sol-gel chemistry were employed to chemically bind a hydroxy-terminated silicone polymer (polydimethyldiphenylsiloxane, PDMDPS) to a sol-gel zirconia network in the course of its evolution from a highly reactive alkoxide precursor undergoing controlled hydrolytic polycondensation reactions. A fused silica capillary was filled with a properly designed sol solution to allow for the sol-gel reactions to take place within the capillary for a predetermined period of time (typically 15-30 min). In the course of this process, a layer of the evolving hybrid organic-inorganic sol-gel polymer got chemically anchored to the silanol groups on the capillary inner walls via condensation reaction. At the end of this in-capillary residence time, the unbonded part of the sol solution was expelled from the capillary under helium pressure, leaving behind a chemically bonded sol-gel zirconia-PDMDPS coating on the inner walls. Polycyclic aromatic hydrocarbons, ketones, and aldehydes were efficiently extracted and preconcentrated from dilute aqueous samples using sol-gel zirconia-PDMDPS coated capillaries followed by thermal desorption and GC analysis of the extracted solutes. The newly developed sol-gel hybrid zirconia coatings demonstrated excellent pH stability, and retained the extraction characteristics intact even after continuous rinsing with a 0.1 M NaOH solution for 24 h. To our knowledge, this is the first report on the use of a sol-gel zirconia-based hybrid organic-inorganic coating as an extraction medium in solid phase microextraction (SPME).

Applications of liquid-based separation in conjunction with mass spectrometry to the analysis of forensic evidence.

PubMed

Moini, Mehdi

2018-05-01

In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives. To this end, the application of separation techniques in conjunction with mass spectrometry has played an important role in the analysis of forensic evidence. Moreover, in the past few years the role of liquid separation techniques, such as liquid chromatography and capillary electrophoresis in conjunction with mass spectrometry, has gained significant tractions and have been applied to a wide range of chemicals, from small molecules such as drugs and explosives, to large molecules such as proteins. For example, proteomics and peptidomics have been used for identification of humans, organs, and bodily fluids. A wide range of HPLC techniques including reversed phase, hydrophilic interaction, mixed-mode, supercritical fluid, multidimensional chromatography, and nanoLC, as well as several modes of capillary electrophoresis mass spectrometry, including capillary zone electrophoresis, partial filling, full filling, and micellar electrokenetic chromatography have been applied to the analysis drugs, explosives, and questioned documents. In this article, we review recent (2015-2017) applications of liquid separation in conjunction with mass spectrometry to the analysis of forensic evidence. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The direct determination of dose-to-water using a water calorimeter.

PubMed

Schulz, R J; Wuu, C S; Weinhous, M S

1987-01-01

A flexible, temperature-regulated, water calorimeter has been constructed which consists of three nested cylinders. The innermost "core" is a 10 X 10 cm right cylinder made of glass, the contents of which are isolated from the environment. It has two Teflon-washered glass valves for filling, and two thermistors are supported at the center by glass capillary tubes. Surrounding the core is a "jacket" that provides approximately 2 cm of air insulation between the core and the "shield." The shield surrounds the jacket with a 2.5-cm layer of temperature-regulated water flowing at 51/min. The core is filled with highly purified water the gas content of which is established prior to filling. Convection currents, which may be induced by dose gradients or thermistor power dissipation, are eliminated by operating the calorimeter at 4 degrees C. Depending upon the power level of the thermistors, 15-200 microW, and the insulation provided by the glass capillary tubing, the temperature of the thermistors is higher than that of the surrounding water. To minimize potential errors caused by differences between calibration curves obtained at finite power levels, the zero-power-level calibration curve obtained by extrapolation is employed. Also the calorimeter response is corrected for the change in power level, and therefore thermistor temperature, that follows the resistance change caused by irradiation. The response of the calorimeter to 4-MV x rays has been compared to that of an ionization chamber irradiated in an identical geometry.(ABSTRACT TRUNCATED AT 250 WORDS)

Surfactants non-monotonically modify the onset of Faraday waves

NASA Astrophysics Data System (ADS)

Strickland, Stephen; Shearer, Michael; Daniels, Karen

2017-11-01

When a water-filled container is vertically vibrated, subharmonic Faraday waves emerge once the driving from the vibrations exceeds viscous dissipation. In the presence of an insoluble surfactant, a viscous boundary layer forms at the contaminated surface to balance the Marangoni and Boussinesq stresses. For linear gravity-capillary waves in an undriven fluid, the surfactant-induced boundary layer increases the amount of viscous dissipation. In our analysis and experiments, we consider whether similar effects occur for nonlinear Faraday (gravity-capillary) waves. Assuming a finite-depth, infinite-breadth, low-viscosity fluid, we derive an analytic expression for the onset acceleration up to second order in ɛ =√{ 1 / Re } . This expression allows us to include fluid depth and driving frequency as parameters, in addition to the Marangoni and Boussinesq numbers. For millimetric fluid depths and driving frequencies of 30 to 120 Hz, our analysis recovers prior numerical results and agrees with our measurements of NBD-PC surfactant on DI water. In both case, the onset acceleration increases non-monotonically as a function of Marangoni and Boussinesq numbers. For shallower systems, our model predicts that surfactants could decrease the onset acceleration. DMS-0968258.

Influence of laser wavelength and pulse duration on gas bubble formation in blood filled glass capillaries.

PubMed

Kimel, Sol; Choi, Bernard; Svaasand, Lars O; Lotfi, Justin; Viator, John A; Nelson, J Stuart

2005-04-01

Hypervascular skin lesions (HVSL) are treated with medical lasers characterized by a variety of parameters such as wavelength lambda, pulse duration t(p), and radiant exposure E that can be adjusted for different pathology and blood vessel size. Treatment parameters have been optimized assuming constant optical properties of blood during laser photocoagulation. However, recent studies suggest that this assumption may not always be true. Our objective was to quantify thermally induced changes in blood that occur during irradiation using standard laser parameters. Glass capillary tubes (diameter D = 100, 200, and 337 microm) filled with fresh or hemolyzed rabbit blood were irradiated once at lambda = 585, 595, or 600 nm, t(p) = 1.5 milliseconds; and also at lambda = 585 nm, t(p) = 0.45 milliseconds. E was increased until blood ablation caused formation of permanent gas bubbles. In a corroborative study, human blood was heated at 50 degrees C and absorbance spectra were measured as a function of time. Threshold radiant exposure, E(thresh), for gas bubble formation was found not to depend on lambda, which might be surprising in view of the 10-fold lower absorption coefficient at 600 nm as compared to 585 nm. The spectroscopic study revealed heat-induced changes in blood constituent composition of hemoglobins (Hb) from initially 100% oxyhemoglobin (HbO2) to deoxyhemoglobin (HHb) and, ultimately, methemoglobin (metHb) as the major constituent. Model calculations of E(thresh)(lambda,D) based on changing constituent blood composition during heating with milliseconds lasers were found to correlate with experimental results. For laser treatment of HVSL it appears that lambda is of secondary importance and that the choice of t(p) is a more important factor. Copyright 2005 Wiley-Liss, Inc.

Experimental investigations on sodium-filled heat pipes

NASA Technical Reports Server (NTRS)

Dorner, S.; Reiss, F.; Schretzmann, K.

1977-01-01

The possibilities of producing heat pipes and, especially, the necessary capillary structures are discussed. Several types of heat pipes were made from stainless steel and tested at temperatures between 400 and 1055 deg C. The thermal power was determined by a calorimeter. Results indicate: bubble-free evaporation of sodium from rectangular open chennels is possible with a heat flux of more than 1,940 W/sq cm at 1055 C. The temperature drop along the tube could be measured only at low temperatures. A subdivided heat pipe worked against the gravitational field. A heat pipe with a capillary structure made of a rolled screen was supported by rings and bars operated at 250 W/sq cm heat flux in the evaporating region.

Powerless tunable photonic crystal with bistable color and millisecond switching.

PubMed

Chan, Chia-Tsung; Yeh, J Andrew

2011-07-04

This study demonstrated a tunable photonic crystal (PhC) with 70 nm-wide spectral tuning (535 nm to 605 nm) and 3 ms of response time. The tunable PhC is based on reciprocal capillary action of liquid in the nanoscale PhC voids. By wetting the porous silicon PhC with ethanol and water, the PhC can be bistably switched respectively between liquid-filled state (orange color) and vapor-filled state (yellow color). Owing to the energy barrier between the two wetting states, the tunable PhC can remain at either of the two states with no external power consumption.

Jet pump assisted artery

NASA Technical Reports Server (NTRS)

1975-01-01

A procedure for priming an arterial heat pump is reported; the procedure also has a means for maintaining the pump in a primed state. This concept utilizes a capillary driven jet pump to create the necessary suction to fill the artery. Basically, the jet pump consists of a venturi or nozzle-diffuser type constriction in the vapor passage. The throat of this venturi is connected to the artery. Thus vapor, gas, liquid, or a combination of the above is pumped continuously out of the artery. As a result, the artery is always filled with liquid and an adequate supply of working fluid is provided to the evaporator of the heat pipe.

A computational DFT study of structural transitions in textured solid-fluid interfaces

NASA Astrophysics Data System (ADS)

Yatsyshin, Petr; Parry, Andrew O.; Kalliadasis, Serafim

2015-11-01

Fluids adsorbed at walls, in capillary pores and slits, and in more exotic, sculpted geometries such as grooves and wedges can exhibit many new phase transitions, including wetting, pre-wetting, capillary-condensation and filling, compared to their bulk counterparts. As well as being of fundamental interest to the modern statistical mechanical theory of inhomogeneous fluids, these are also relevant to nanofluidics, chemical- and bioengineering. In this talk we will show using a microscopic Density Functional Theory (DFT) for fluids how novel, continuous, interfacial transitions associated with the first-order prewetting line, can occur on steps, in grooves and in wedges, that are sensitive to both the range of the intermolecular forces and interfacial fluctuation effects. These transitions compete with wetting, filling and condensation producing very rich phase diagrams even for relatively simple geometries. We will also discuss practical aspects of DFT calculations, and demonstrate how this statistical-mechanical framework is capable of yielding complex fluid structure, interfacial tensions, and regions of thermodynamic stability of various fluid configurations. As a side note, this demonstrates that DFT is an excellent tool for the investigations of complex multiphase systems. We acknowledge financial support from the European Research Council via Advanced Grant No. 247031.

Effect of Capillary Tube’s Shape on Capillary Rising Regime for Viscos Fluids

NASA Astrophysics Data System (ADS)

Soroush, F.; Moosavi, A.

2018-05-01

When properties of the displacing fluid are considered, the rising profile of the penetrating fluid in a capillary tube deviates from its classical Lucas-Washburn profile. Also, shape of capillary tube can affect the rising profile in different aspects. In this article, effect of capillary tube’s shape on the vertical capillary motion in presence of gravity is investigated by considering the properties of the displacing fluid. According to the fact that the differential equation of the capillary rising for a non-simple wall type is very difficult to solve analytically, a finite element simulation model is used for this study. After validation of the simulation model with an experiment that has been done with a simple capillary tube, shape of the capillary tube’s wall is changed in order to understand its effects on the capillary rising and different motion regimes that may appear according to different geometries. The main focus of this article is on the sinusoidal wall shapes and comparing them with a simple wall.

A simulation model of the oxygen alveolo-capillary exchange in normal and pathological conditions.

PubMed

Brighenti, Chiara; Gnudi, Gianni; Avanzolini, Guido

2003-05-01

This paper presents a mathematical model of the oxygen alveolo-capillary exchange to provide the capillary oxygen partial pressure profile in normal and pathological conditions. In fact, a thickening of the blood-gas barrier, heavy exercise or a low oxygen partial pressure (PO2) in the alveolar space can reduce the O2 alveolo-capillary exchange. Since the reversible binding between haemoglobin and oxygen makes it impossible to determine the closed form for the mathematical description of the PO2 profile along the pulmonary capillaries, an approximate analytical solution of the capillary PO2 profile is proposed. Simulation results are compared with the capillary PO2 profile obtained by numerical integration and by a piecewise linear interpolation of the oxyhaemoglobin dissociation curve. Finally, the proposed model is evaluated in a large range of physiopathological diffusive conditions. The good fit to numerical solutions in all experimental conditions seems to represent a substantial improvement with respect to the approach based on a linear approximation of the oxyhaemoglobin dissociation curve, and makes this model a candidate to be incorporated into the integrated descriptions of the entire respiratory system, where the datum of primary interest is the value of end capillary PO2.

Capillary rise in a textured channel

NASA Astrophysics Data System (ADS)

Beilharz, Daniel; Clanet, Christophe; Quere, David

2016-11-01

A wetting liquid can invade a textured material, for example a forest of micropillars. The driving and the viscous forces of this motion are determined by the texture parameters and the influence of shape, height and spacing of posts has been widely studied for the last decade. In this work, we build a channel with textured walls. Brought into contact with a reservoir of wetting liquid, we observe in some cases two advancing fronts. A first one ahead invading the forest of micropillars, and a second one behind filling the remaining gap. We study and model the conditions of existence and the dynamics of these two fronts as a function of the characteristics of both microstructure and gap of this elementary porous medium.

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips.

PubMed

Deng, Yongbo; Fan, Jianhua; Zhou, Song; Zhou, Teng; Wu, Junfeng; Li, Yin; Liu, Zhenyu; Xuan, Ming; Wu, Yihui

2014-03-01

Based on the Euler force induced by the acceleration of compact disk (CD)-like microfluidic chip, this paper presents a novel actuation mechanism for siphon valving. At the preliminary stage of acceleration, the Euler force in the tangential direction of CD-like chip takes the primary place compared with the centrifugal force to function as the actuation of the flow, which fills the siphon and actuates the siphon valving. The Euler force actuation mechanism is demonstrated by the numerical solution of the phase-field based mathematical model for the flow in siphon valve. In addition, experimental validation is implemented in the polymethylmethacrylate-based CD-like microfluidic chip manufactured using CO2 laser engraving technique. To prove the application of the proposed Euler force actuation mechanism, whole blood separation and plasma extraction has been conducted using the Euler force actuated siphon valving. The newly introduced actuation mechanism overcomes the dependence on hydrophilic capillary filling of siphon by avoiding external manipulation or surface treatments of polymeric material. The sacrifice for highly integrated processing in pneumatic pumping technique is also prevented by excluding the volume-occupied compressed air chamber.

Invasion of gas into mica nanopores: a molecular dynamics study

NASA Astrophysics Data System (ADS)

Fang, Chao; Zhang, Fei; Qiao, Rui

2018-06-01

The invasion of gas into liquid-filled nanopores is encountered in many engineering problems but is not yet well understood. We report molecular dynamics simulations of the invasion of methane gas into water-filled mica pores with widths of 2–6 nm. Gas invades into a pore only when the pressure exceeds a breakthrough pressure and a thin residual water film is left on the mica wall as the gas phase moves deeper into the pore. The gas breakthrough pressure of pores as narrow as 2 nm can be modeled reasonably well by the capillary pressure if the finite thickness of residual liquid water film and the liquid–gas interface are taken into account. The movement of the front of the liquid meniscus during gas invasion can be quantitatively described using the classical hydrodynamics when the negative slip length on the strongly hydrophilic mica walls is taken into account. Understanding the molecular mechanisms underlying the gas invasion in the system studied here will form the foundation for quantitative prediction of gas invasion in practical porous media.

Effect of using acetone and distilled water on the performance of open loop pulsating heat pipe (OLPHP) with different filling ratios

NASA Astrophysics Data System (ADS)

Rahman, Md. Lutfor; Afrose, Tonima; Tahmina, Halima Khatun; Rinky, Rumana Parvin; Ali, Mohammad

2016-07-01

Pulsating heat pipe (PHP) is a new innovation in the modern era of miniaturizes thermal management system for its higher heating and cooling capacity. The objective of this experiment is to observe the performance of open loop pulsating heat pipe using two fluids at different filling ratios. This OLPHP is a copper capillary tube of 2.5mm outer diameter and 2mm inner diameter. It consists of 8 loops where the evaporative section is 50mm, adiabatic section is 120mm and condensation section is 80mm. The experiment is conducted with distilled water and acetone at 40%, 50%, 60%, and 70% filling ratios where 0° (vertical) is considered as definite angle of inclination. Distilled water and acetone are selected as working fluids considering their different latent heat of vaporization and surface tension. It is found that acetone shows lower thermal resistance than water at all heat inputs. Best performance of acetone is attained at 70% filling ratio. Water displays better heat transfer capability at 50% filling ratio.

Monogroove liquid heat exchanger

NASA Technical Reports Server (NTRS)

Brown, Richard F. (Inventor); Edelstein, Fred (Inventor)

1990-01-01

A liquid supply control is disclosed for a heat transfer system which transports heat by liquid-vapor phase change of a working fluid. An assembly (10) of monogroove heat pipe legs (15) can be operated automatically as either heat acquisition devices or heat discharge sources. The liquid channels (27) of the heat pipe legs (15) are connected to a reservoir (35) which is filled and drained by respective filling and draining valves (30, 32). Information from liquid level sensors (50, 51) on the reservoir (35) is combined (60) with temperature information (55) from the liquid heat exchanger (12) and temperature information (56) from the assembly vapor conduit (42) to regulate filling and draining of the reservoir (35), so that the reservoir (35) in turn serves the liquid supply/drain needs of the heat pipe legs (15), on demand, by passive capillary action (20, 28).

A composite smeared finite element for mass transport in capillary systems and biological tissue.

PubMed

Kojic, M; Milosevic, M; Simic, V; Koay, E J; Fleming, J B; Nizzero, S; Kojic, N; Ziemys, A; Ferrari, M

2017-09-01

One of the key processes in living organisms is mass transport occurring from blood vessels to tissues for supplying tissues with oxygen, nutrients, drugs, immune cells, and - in the reverse direction - transport of waste products of cell metabolism to blood vessels. The mass exchange from blood vessels to tissue and vice versa occurs through blood vessel walls. This vital process has been investigated experimentally over centuries, and also in the last decades by the use of computational methods. Due to geometrical and functional complexity and heterogeneity of capillary systems, it is however not feasible to model in silico individual capillaries (including transport through the walls and coupling to tissue) within whole organ models. Hence, there is a need for simplified and robust computational models that address mass transport in capillary-tissue systems. We here introduce a smeared modeling concept for gradient-driven mass transport and formulate a new composite smeared finite element (CSFE). The transport from capillary system is first smeared to continuous mass sources within tissue, under the assumption of uniform concentration within capillaries. Here, the fundamental relation between capillary surface area and volumetric fraction is derived as the basis for modeling transport through capillary walls. Further, we formulate the CSFE which relies on the transformation of the one-dimensional (1D) constitutive relations (for transport within capillaries) into the continuum form expressed by Darcy's and diffusion tensors. The introduced CSFE is composed of two volumetric parts - capillary and tissue domains, and has four nodal degrees of freedom (DOF): pressure and concentration for each of the two domains. The domains are coupled by connectivity elements at each node. The fictitious connectivity elements take into account the surface area of capillary walls which belongs to each node, as well as the wall material properties (permeability and partitioning). The overall FE model contains geometrical and material characteristics of the entire capillary-tissue system, with physiologically measurable parameters assigned to each FE node within the model. The smeared concept is implemented into our implicit-iterative FE scheme and into FE package PAK. The first three examples illustrate accuracy of the CSFE element, while the liver and pancreas models demonstrate robustness of the introduced methodology and its applicability to real physiological conditions.

[Key factors in the control of electroosmosis with external radial electric field in CE].

PubMed

Zhu, Y; Chen, Y

1999-11-01

Direct control of electroosmosis flow (EOF) by external radial electric field was performed at room temperature using a home-made field-modulated capillary electrophoresis (CE) system. The EOF was monitored at 206 nm by using DMSO as a probe. To apply a radial electric field across the CE capillary wall, the capillary was cased with a wide column. Both of the concentric space and the capillary bore were then filled with an identical running buffer and applied with an axial electric field of 150 V/cm but starting from different levels. All of the tubes used were made of fused silica with polyimide over-coating (from the Yongnian Optical Fiber Work, Hebei, P. R. China). The size of the CE capillaries adopted was 25-100 microns i.d. (375 microns o.d.) x 28.5/45 cm (effective/total length), and that of the casing column 400 microns i.d. x 32 cm. To investigate the fundamentals of the external EOF control when using the flexible fused silica capillaries, various parameters have been inspected such as pH, buffer composition, additives and capillary wall feature etc.. As expected, to well control both of the magnitude and direction of the electroosmosis, the buffer pH should be kept below 4 and the buffer concentration below 50 mmol/L. However, buffers below 1 mmol/L should be avoided because such a diluted running buffer may result in poor CE separation. Weak electrolytes like citric acid, tartaric acid and acetic acid were found to be capable of generating better EOF control than the strong electrolytes such as phosphate and chlorides. This is possibly due to the formation of looser electric double layer with the weak rather than the strong electrolytes. Some wall coatings like calix arene and its derivatives can evidently improve the EOF control even at pH 5. This reveals an exciting way to expend the controllable pH range. In addition, narrow-bore capillaries were demonstrated to be better than wide-bore tubes. Other conditions such as buffer additives and capillary rinse procedure were shown to have only negligible influence on the control.

Current characteristics of λ-DNA molecules/polystyrene nanoparticles in TBE buffer solution through micro/nanofluidic capillaries under DC electric field

NASA Astrophysics Data System (ADS)

Duan, Yifei; Zhao, Wei; Xue, Jing; Sun, Dan; Wang, Kaige; Wang, Guiren; Li, Junjie; Bai, Jintao; Gu, Changzhi

2017-03-01

In practical applications of biochips and bio-sensors, electrokinetic mechanisms are commonly employed to manipulate single bio-molecules and analyze their characteristics. To accurately and flexibly control the movement of single-molecule within micro/nanofluidic channels which are the basic components of Lab-chips, the current signals in micro/nanocapillaries filled with solutions of DNA molecules or polystyrene (PS) nanoparticles are systematically studied. Experimental results indicate that the current response along the micro/nanocapillaries can be significantly influenced by the diameter of the capillaries and the pH value of the solutions. Specifically, when there is only a pure (TBE) solution, the electric conductance does not monotonically decrease with decreasing the diameter of the capillaries, but slightly increases with decreasing the capillary diameter. When λ-DNA molecules or PS nanoparticles are added into the TBE buffer, the size effect on the electric conductance of the solutions are quite different. Although in the former, the electric conductance behaves differently from that in the pure TBE solution and decreases with the decreasing diameter, in the latter, the change is similar to that in the pure TBE solution. Besides, an abnormal ‘falling’ of the electric conductance is observed in a capillary with diameter of 200 nm. The investigation will significantly enhance the understanding on the electric properties of the solutions of biomolecules and particles in micro/nanofluidics. This is especially helpful for designing functional Lab-chip devices.

Micro-channel filling flow considering surface tension effect

NASA Astrophysics Data System (ADS)

Kim, Dong Sung; Lee, Kwang-Cheol; Kwon, Tai Hun; Lee, Seung S.

2002-05-01

Understanding filling flow into micro-channels is important in designing micro-injection molding, micro-fluidic devices and an MIMIC (micromolding in capillaries) process. In this paper, we investigated, both experimentally and numerically, 'transient filling' flow into micro-channels, which differs from steady-state completely 'filled' flow in micro-channels. An experimental flow visualization system was devised to facilitate observation of flow characteristics in filling into micro-channels. Three sets of micro-channels of various widths of different thicknesses (20, 30, and 40 μm) were fabricated using SU-8 on the silicon substrate to find a geometric effect with regard to pressure gradient, viscous force and, in particular, surface tension. A numerical analysis system has also been developed taking into account the surface tension effect with a contact angle concept. Experimental observations indicate that surface tension significantly affects the filling flow to such an extent that even a flow blockage phenomenon was observed at channels of small width and thickness. A numerical analysis system also confirms that the flow blockage phenomenon could take place due to the flow hindrance effect of surface tension, which is consistent with experimental observation. For proper numerical simulations, two correction factors have also been proposed to correct the conventional hydraulic radius for the filling flow in rectangular cross-sectioned channels.

Sol-gel chemistry-based Ucon-coated columns for capillary electrophoresis.

PubMed

Hayes, J D; Malik, A

1997-07-18

A sol-gel chemistry-based novel approach for the preparation of a Ucon-coated fused-silica capillary column in capillary electrophoresis is presented. In this approach the sol-gel process is carried out inside 25 microm I.D. fused-silica capillaries. The sol solution contained appropriate quantities of an alkoxide-based sol-gel precursor, a polymeric coating material (Ucon), a crosslinking reagent, a surface derivatizing reagent, controlled amounts of water and a catalyst dissolved in a suitable solvent system. The coating procedure involves filling a capillary with the sol solution and allowing the sol-gel process to proceed for an optimum period. Hydrolysis of the alkoxide precursor and polycondensation of the hydrolyzed products with the surface silanol groups and the hydroxy-terminated Ucon molecules lead to the formation of a surface-bonded sol-gel coating on the inner walls of the capillary. The thickness of the coated film can be controlled by varying the reaction time, coating solution composition and experimental conditions. Commercial availability of high purity sol-gel precursors (e.g., TEOS 99.999%), the ease of coating, run-to-run and column-to-column reproducibility, and long column lifetimes make sol-gel coating chemistry very much suitable for being applied in analytical microseparations column technology. Test samples of basic proteins and nucleotides were used to evaluate the column performance. These results show that the sol-gel coating scheme has allowed for the generation of bio-compatible surfaces characterized by high separation efficiencies in CE. For different types of solutes, the sol-gel coated Ucon column consistently provided migration time R.S.D. values of the order of 0.5%.

Terrestrial Testing of the CapiBRIC, a Microgravity Optimized Brine Processor

NASA Technical Reports Server (NTRS)

Sargusingh, Miriam J.; Callahan, Michael R.; Weislogel, Mark M.

2016-01-01

Utilizing geometry based static phase separation exhibited in the radial vaned capillary drying tray, a system was conceived to recover water from brine. This technology has been named the Capillary BRIC; abbreviated CapiBRIC. The CapiBRIC utilizes a capillary drying tray within a drying chamber. Water is recovered from clean water vapor evaporating from the free surface leaving waste brine solids behind. A novel approach of optimizing the containment geometry to support passive capillary flow and static phase separation provides the opportunity for a low power system that is not as susceptible to fouling as membranes or other technologies employing physical barriers across the free brine surface to achieve phase separation in microgravity. Having been optimized for operation in microgravity, full-scale testing of the CapiBRIC as designed cannot be performed on the ground as the force of gravity would dominate over the capillary forces. However, subscale units relevant to full-scale design were used to characterize fill rates, containment stability, and interaction with a variable volume reservoir in the PSU Dryden Drop Tower (DDT) facility. PSU also using tested units scaled such that capillary forces dominated in a 1-g environment to characterize evaporation from a free-surface in 1-g upward, sideways and downward orientations. In order to augment the subscale testing performed by PSU, a full scale 1-g analogue of the CapiBRIC drying unit was initiated to help validate performance predictions regarding expected water recovery ratio, estimated processing time, and interface definitions for inlets, outlets, and internal processes, including vent gas composition. This paper describes the design, development and test of the terrestrial CapiBRIC prototypes.

Continuous condensation in nanogrooves

NASA Astrophysics Data System (ADS)

Malijevský, Alexandr

2018-05-01

We consider condensation in a capillary groove of width L and depth D , formed by walls that are completely wet (contact angle θ =0 ), which is in a contact with a gas reservoir of the chemical potential μ . On a mesoscopic level, the condensation process can be described in terms of the midpoint height ℓ of a meniscus formed at the liquid-gas interface. For macroscopically deep grooves (D →∞ ), and in the presence of long-range (dispersion) forces, the condensation corresponds to a second-order phase transition, such that ℓ ˜(μcc-μ ) -1 /4 as μ →μcc - where μc c is the chemical potential pertinent to capillary condensation in a slit pore of width L . For finite values of D , the transition becomes rounded and the groove becomes filled with liquid at a chemical potential higher than μc c with a difference of the order of D-3. For sufficiently deep grooves, the meniscus growth initially follows the power law ℓ ˜(μcc-μ ) -1 /4 , but this behavior eventually crosses over to ℓ ˜D -(μ-μc c) -1 /3 above μc c, with a gap between the two regimes shown to be δ ¯μ ˜D-3 . Right at μ =μc c , when the groove is only partially filled with liquid, the height of the meniscus scales as ℓ*˜(D3L) 1 /4 . Moreover, the chemical potential (or pressure) at which the groove is half-filled with liquid exhibits a nonmonotonic dependence on D with a maximum at D ≈3 L /2 and coincides with μc c when L ≈D . Finally, we show that condensation in finite grooves can be mapped on the condensation in capillary slits formed by two asymmetric (competing) walls a distance D apart with potential strengths depending on L . All these predictions, based on mesoscopic arguments, are confirmed by fully microscopic Rosenfeld's density functional theory with a reasonable agreement down to surprisingly small values of both L and D .

Analysis of Tank PMD Rewetting Following Thrust Resettling

NASA Astrophysics Data System (ADS)

Weislogel, M. M.; Sala, M. A.; Collicott, S. H.

2002-10-01

Recent investigations have successfully demonstrated closed-form analytical solutions of spontaneous capillary flows in idealized cylindrical containers with interior corners. In this report, the theory is extended and applied to complex containers modeling spacecraft fuel tanks employing propellant management devices (PMDs). The specific problem investigated is one of spontaneous rewetting of a typical partially filled liquid fuel/cryogen tank with PMD after thrust resettling. The transients of this flow impact the logistics of orbital maneuvers and potentially tank thermal control. The general procedure to compute the initial condition (mean radius of curvature for the interface) for the closed-form transient flows is first outlined then solved for several 'complex' cylindrical tanks exhibiting symmetry. The utility and limitations of the technique as a design tool are discussed in a summary, which also highlights comparisons with NASA flight data of a model propellant tank with PMD.

Analysis of Tank PMD Rewetting Following Thrust Resettling

NASA Technical Reports Server (NTRS)

Weislogel, M. M.; Sala, M. A.; Collicott, S. H.; Rame, Enrique (Technical Monitor)

2002-01-01

Recent investigations have successfully demonstrated closed-form analytical solutions of spontaneous capillary flows in idealized cylindrical containers with interior corners. In this report, the theory is extended and applied to complex containers modeling spacecraft fuel tanks employing propellant management devices (PMDs). The specific problem investigated is one of spontaneous rewetting of a typical partially filled liquid fuel/cryogen tank with PMD after thrust resettling. The transients of this flow impact the logistics of orbital maneuvers and potentially tank thermal control. The general procedure to compute the initial condition (mean radius of curvature for the interface) for the closed-form transient flows is first outlined then solved for several 'complex' cylindrical tanks exhibiting symmetry. The utility and limitations of the technique as a design tool are discussed in a summary, which also highlights comparisons with NASA flight data of a model propellant tank with PMD.

Multicomponent micropatterned sol-gel materials by capillary molding

NASA Astrophysics Data System (ADS)

Lochhead, Michael J.; Yager, Paul

1997-10-01

A physically and chemically benign method for patterning multiple sol-gel materials onto a single substrate is described. Structures are demonstrated for potential micro- optical chemical sensor, biosensor, and waveguiding applications. Fabrication is based on the micro molding in capillaries (MIMIC) approach. A novel mold design allows several sols to be cast simultaneously. Closely spaced, organically modified silica ridges containing fluorescent dyes are demonstrated. Ridges have cross sectional dimensions from one to 50 micrometers and are centimeters in length. Processing issues, particularly those related to mold filling, are discussed in detail. Because sol-gel MIMIC avoids the harsh physical and chemical environments normally associated with patterning, the approach allows full exploitation of sol- gel processing advantages, such as the ability to entrap sensitive organic dopant molecules in the sol-gel matrix.

Copolymers For Capillary Gel Electrophoresis

DOEpatents

Liu, Changsheng; Li, Qingbo

2005-08-09

This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.

SPH-DEM approach to numerically simulate the deformation of three-dimensional RBCs in non-uniform capillaries.

PubMed

Polwaththe-Gallage, Hasitha-Nayanajith; Saha, Suvash C; Sauret, Emilie; Flower, Robert; Senadeera, Wijitha; Gu, YuanTong

2016-12-28

Blood continuously flows through the blood vessels in the human body. When blood flows through the smallest blood vessels, red blood cells (RBCs) in the blood exhibit various types of motion and deformed shapes. Computational modelling techniques can be used to successfully predict the behaviour of the RBCs in capillaries. In this study, we report the application of a meshfree particle approach to model and predict the motion and deformation of three-dimensional RBCs in capillaries. An elastic spring network based on the discrete element method (DEM) is employed to model the three-dimensional RBC membrane. The haemoglobin in the RBC and the plasma in the blood are modelled as smoothed particle hydrodynamics (SPH) particles. For validation purposes, the behaviour of a single RBC in a simple shear flow is examined and compared against experimental results. Then simulations are carried out to predict the behaviour of RBCs in a capillary; (i) the motion of five identical RBCs in a uniform capillary, (ii) the motion of five identical RBCs with different bending stiffness (K b ) values in a stenosed capillary, (iii) the motion of three RBCs in a narrow capillary. Finally five identical RBCs are employed to determine the critical diameter of a stenosed capillary. Validation results showed a good agreement with less than 10% difference. From the above simulations, the following results are obtained; (i) RBCs exhibit different deformation behaviours due to the hydrodynamic interaction between them. (ii) Asymmetrical deformation behaviours of the RBCs are clearly observed when the bending stiffness (K b ) of the RBCs is changed. (iii) The model predicts the ability of the RBCs to squeeze through smaller blood vessels. Finally, from the simulations, the critical diameter of the stenosed section to stop the motion of blood flow is predicted. A three-dimensional spring network model based on DEM in combination with the SPH method is successfully used to model the motion and deformation of RBCs in capillaries. Simulation results reveal that the condition of blood flow stopping depends on the pressure gradient of the capillary and the severity of stenosis of the capillary. In addition, this model is capable of predicting the critical diameter which prevents motion of RBCs for different blood pressures.

Modeling of the capillary wicking of flax fibers by considering the effects of fiber swelling and liquid absorption.

PubMed

Testoni, Guilherme Apolinario; Kim, Sihwan; Pisupati, Anurag; Park, Chung Hae

2018-09-01

We propose a new model for the capillary rise of liquid in flax fibers whose diameter is changed by liquid absorption. Liquid absorption into the flax fibers is taken into account in a new modified Washburn equation by considering the mass of the liquid absorbed inside the fibers as well as that imbibed between the fibers. The change of permeability and hydraulic radius of pores in a fibrous medium due to the fiber swelling is modeled by a statistical approach considering a non-uniform distribution of flax fiber diameter. By comparisons between capillary rise test results and modeling results, we prove the validity of the proposed modified Washburn model to take into account the effects from fiber swelling and liquid absorption on the decrease of capillary rise velocity. The experimental observation of long-term capillary rise tests show that the swelling behavior of the fibers highly packed in a closed volume and its influence on the capillary wicking are different from those of an individual single fiber in a free space. The current approach was useful to characterize the swelling of fibers highly packed in a closed volume and its influence of the long-term behavior of capillary wicking. Copyright © 2018 Elsevier Inc. All rights reserved.

Analysis of microdialysate monoamines, including noradrenaline, dopamine and serotonin, using capillary ultra-high performance liquid chromatography and electrochemical detection.

PubMed

Ferry, Barbara; Gifu, Elena-Patricia; Sandu, Ioana; Denoroy, Luc; Parrot, Sandrine

2014-03-01

Electrochemical methods are very often used to detect catecholamine and indolamine neurotransmitters separated by conventional reverse-phase high performance liquid chromatography (HPLC). The present paper presents the development of a chromatographic method to detect monoamines present in low-volume brain dialysis samples using a capillary column filled with sub-2μm particles. Several parameters (repeatability, linearity, accuracy, limit of detection) for this new ultrahigh performance liquid chromatography (UHPLC) method with electrochemical detection were examined after optimization of the analytical conditions. Noradrenaline, adrenaline, serotonin, dopamine and its metabolite 3-methoxytyramine were separated in 1μL of injected sample volume; they were detected above concentrations of 0.5-1nmol/L, with 2.1-9.5% accuracy and intra-assay repeatability equal to or less than 6%. The final method was applied to very low volume dialysates from rat brain containing monoamine traces. The study demonstrates that capillary UHPLC with electrochemical detection is suitable for monitoring dialysate monoamines collected at high sampling rate. Copyright © 2014 Elsevier B.V. All rights reserved.

Squeezing and de-wetting of a shear thinning fluid drop between plane parallel surfaces: capillary adhesion phenomenon

NASA Astrophysics Data System (ADS)

Ward, Thomas

2017-11-01

The radial squeezing and de-wetting of a thin film of viscous shear thinning fluid filling the gap between parallel plane walls is examined both experimentally and theoretically for gap spacing much smaller than the capillary length. The interaction between motion of fluid in the gap driven by squeezing or de-wetting and surface tension is parameterized by a dimensionless variable, F, that is the ratio of the constant force supplied by the top plate (either positive or negative) to surface tension at the drop's circumference. Furthermore, the dimensionless form of the rate equation for the gap's motion reveals a time scale that is dependent on the drop volume when analyzed for a power law shear thinning fluid. In the de-wetting problem the analytical solution reveals the formation of a singularity, leading to capillary adhesion, as the gap spacing approaches a critical value that depends on F and the contact angle. Experiments are performed to test the analytical predictions for both squeezing, and de-wetting in the vicinity of the singularity.

Water imbibition by mica pores: what happens when capillary flow is suppressed?

NASA Astrophysics Data System (ADS)

Fang, Chao; Qiao, Rui

2017-11-01

The imbibition of liquids into porous media plays a critical role in numerous applications. Most prior studies focused on imbibition driven by capillary flows. In this work, we study the imbibition of water into slit-shaped mica pores filled with pressurized methane using molecular simulations. Despite that capillary flow is suppressed by the high gas pressure, water is imbibed into the pore as monolayer liquid films. Since the classical hydrodynamic flow is not readily applicable for the monolayer water film propagating on the mica wall and the imbibition is driven by the strong affinity of water molecules to the mica walls, the observed imbibition is best taken as surface hydration. We show that the dynamics of water's imbibition front follows a simple diffusive scaling law. The effective diffusion coefficient of the imbibition front, however, is more than ten times larger than the diffusion coefficient of the water molecules in the water film adsorbed on the mica walls. Using a molecular theory originally developed for the spreading of monolayer films on solid substrates, we clarify the mechanism underlying the rapid water imbibition observed here.

Microjet formation in a capillary by laser-induced cavitation

NASA Astrophysics Data System (ADS)

Peters, Ivo R.; Tagawa, Yoshiyuki; van der Meer, Devaraj; Prosperetti, Andrea; Sun, Chao; Lohse, Detlef

2010-11-01

A vapor bubble is created by focusing a laser pulse inside a capillary that is partially filled with water. Upon creation of the bubble, a shock wave travels through the capillary. When this shock wave meets the meniscus of the air-water interface, a thin jet is created that travels at very high speeds. A crucial ingredient for the creation of the jet is the shape of the meniscus, which is responsible for focusing the energy provided by the shock wave. We examine the formation of this jet numerically using a boundary integral method, where we prepare an initial interface at rest inside a tube with a diameter ranging from 50 to 500 μm. To simulate the effect of the bubble we then apply a short, strong pressure pulse, after which the jet forms. We investigate the influence of the shape of the meniscus, and pressure amplitude and duration on the jet formation. The jet shape and velocity obtained by the simulation compare well with experimental data, and provides good insight in the origin of the jet.

Superparamagnetic properties of carbon nanotubes filled with NiFe{sub 2}O{sub 4} nanoparticles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stojak Repa, K.; Israel, D.; Phan, M. H., E-mail: phanm@usf.edu, E-mail: sharihar@usf.edu

2015-05-07

Multi walled carbon nanotubes (MWCNTs) were successfully synthesized using custom-made 80 nm pore-size alumina templates, and were uniformly filled with nickel ferrite (NFO) nanoparticles of 7.4 ± 1.7 nm diameter using a novel magnetically assisted capillary action method. X-ray diffraction confirmed the inverse spinel phase for the synthesized NFO. Transmission electron microscopy confirms spherical NFO nanoparticles with an average diameter of 7.4 nm inside MWCNTs. Magnetometry indicates that both NFO and NFO-filled MWCNTs present a blocking temperature around 52 K, with similar superparamagnetic-like behavior, and weak dipolar interactions, giving rise to a super-spin-glass-like behavior at low temperatures. These properties along with the uniformity of sub-100 nm structuresmore » and the possibility of tunable magnetic response in variable diameter carbon nanotubes make them ideal for advanced biomedical and microwave applications.« less

The inclusion of capillary distribution in the adiabatic tissue homogeneity model of blood flow

NASA Astrophysics Data System (ADS)

Koh, T. S.; Zeman, V.; Darko, J.; Lee, T.-Y.; Milosevic, M. F.; Haider, M.; Warde, P.; Yeung, I. W. T.

2001-05-01

We have developed a non-invasive imaging tracer kinetic model for blood flow which takes into account the distribution of capillaries in tissue. Each individual capillary is assumed to follow the adiabatic tissue homogeneity model. The main strength of our new model is in its ability to quantify the functional distribution of capillaries by the standard deviation in the time taken by blood to pass through the tissue. We have applied our model to the human prostate and have tested two different types of distribution functions. Both distribution functions yielded very similar predictions for the various model parameters, and in particular for the standard deviation in transit time. Our motivation for developing this model is the fact that the capillary distribution in cancerous tissue is drastically different from in normal tissue. We believe that there is great potential for our model to be used as a prognostic tool in cancer treatment. For example, an accurate knowledge of the distribution in transit times might result in an accurate estimate of the degree of tumour hypoxia, which is crucial to the success of radiation therapy.

Thermal performance analysis of a flat heat pipe working with carbon nanotube-water nanofluid for cooling of a high heat flux heater

NASA Astrophysics Data System (ADS)

Arya, A.; Sarafraz, M. M.; Shahmiri, S.; Madani, S. A. H.; Nikkhah, V.; Nakhjavani, S. M.

2018-04-01

Experimental investigation on the thermal performance of a flat heat pipe working with carbon nanotube nanofluid is conducted. It is used for cooling a heater working at high heat flux conditions up to 190 kW/m2. The heat pipe is fabricated from aluminium and is equipped with rectangular fin for efficient cooling of condenser section. Inside the heat pipe, a screen mesh was inserted as a wick structure to facilitate the capillary action of working fluid. Influence of different operating parameters such as heat flux, mass concentration of carbon nanotubes and filling ratio of working fluid on thermal performance of heat pipe and its thermal resistance are investigated. Results showed that with an increase in heat flux, the heat transfer coefficient in evaporator section of the heat pipe increases. For filling ratio, however, there is an optimum value, which was 0.8 for the test heat pipe. In addition, CNT/water enhanced the heat transfer coefficient up to 40% over the deionized water. Carbon nanotubes intensified the thermal performance of wick structure by creating a fouling layer on screen mesh structure, which changes the contact angle of liquid with the surface, intensifying the capillary forces.

A novel liquid-Xenon detector concept for combined fast-neutrons and gamma imaging and spectroscopy

NASA Astrophysics Data System (ADS)

Breskin, A.; Israelashvili, I.; Cortesi, M.; Arazi, L.; Shchemelinin, S.; Chechik, R.; Dangendorf, V.; Bromberger, B.; Vartsky, D.

2012-06-01

A new detector concept is presented for combined imaging and spectroscopy of fast-neutrons and gamma rays. It comprises a liquid-Xenon (LXe) converter and scintillator coupled to a UV-sensitive gaseous imaging photomultiplier (GPM). Radiation imaging is obtained by localization of the scintillation-light from LXe with the position-sensitive GPM. The latter comprises a cascade of Thick Gas Electron Multipliers (THGEM), where the first element is coated with a CsI UV-photocathode. We present the concept and provide first model-simulation results of the processes involved and the expected performances of a detector having a LXe-filled capillaries converter. The new detector concept has potential applications in combined fast-neutron and gamma-ray screening of hidden explosives and fissile materials with pulsed sources.

Global patterns of groundwater table depth.

PubMed

Fan, Y; Li, H; Miguez-Macho, G

2013-02-22

Shallow groundwater affects terrestrial ecosystems by sustaining river base-flow and root-zone soil water in the absence of rain, but little is known about the global patterns of water table depth and where it provides vital support for land ecosystems. We present global observations of water table depth compiled from government archives and literature, and fill in data gaps and infer patterns and processes using a groundwater model forced by modern climate, terrain, and sea level. Patterns in water table depth explain patterns in wetlands at the global scale and vegetation gradients at regional and local scales. Overall, shallow groundwater influences 22 to 32% of global land area, including ~15% as groundwater-fed surface water features and 7 to 17% with the water table or its capillary fringe within plant rooting depths.

Blood Perfusion in Microfluidic Models of Pulmonary Capillary Networks: Role of Geometry and Hematocrit

NASA Astrophysics Data System (ADS)

Stauber, Hagit; Waisman, Dan; Sznitman, Josue; Technion-IIT Team; Department of Neonatology Carmel Medical Center; Faculty of Medicine-Technion IIT Collaboration

2015-11-01

Microfluidic platforms are increasingly used to study blood microflows at true physiological scale due to their ability to overcome manufacturing obstacle of complex anatomical morphologies, such as the organ-specific architectures of the microcirculation. In the present work, we utilize microfluidic platforms to devise in vitro models of the underlying pulmonary capillary networks (PCN), where capillary lengths and diameters are similar to the size of RBCs (~ 5-10 μm). To better understand flow characteristics and dispersion of red blood cells (RBCs) in PCNs, we have designed microfluidic models of alveolar capillary beds inspired by the seminal ``sheet flow'' model of Fung and Sobin (1969). Our microfluidic PCNs feature confined arrays of staggered pillars with diameters of ~ 5,7 and 10 μm, mimicking the dense structure of pulmonary capillary meshes. The devices are perfused with suspensions of RBCs at varying hematocrit levels under different flow rates. Whole-field velocity patterns using micro-PIV and single-cell tracking using PTV are obtained with fluorescently-labelled RBCs and discussed. Our experiments deliver a real-scale quantitative description of RBC perfusion characteristics across the pulmonary capillary microcirculation.

Transport through a network of capillaries from ultrametric diffusion equation with quadratic nonlinearity

NASA Astrophysics Data System (ADS)

Oleschko, K.; Khrennikov, A.

2017-10-01

This paper is about a novel mathematical framework to model transport (of, e.g., fluid or gas) through networks of capillaries. This framework takes into account the tree structure of the networks of capillaries. (Roughly speaking, we use the tree-like system of coordinates.) As is well known, tree-geometry can be topologically described as the geometry of an ultrametric space, i.e., a metric space in which the metric satisfies the strong triangle inequality: in each triangle, the third side is less than or equal to the maximum of two other sides. Thus transport (e.g., of oil or emulsion of oil and water in porous media, or blood and air in biological organisms) through networks of capillaries can be mathematically modelled as ultrametric diffusion. Such modelling was performed in a series of recently published papers of the authors. However, the process of transport through capillaries can be only approximately described by the linear diffusion, because the concentration of, e.g., oil droplets, in a capillary can essentially modify the dynamics. Therefore nonlinear dynamical equations provide a more adequate model of transport in a network of capillaries. We consider a nonlinear ultrametric diffusion equation with quadratic nonlinearity - to model transport in such a network. Here, as in the linear case, we apply the theory of ultrametric wavelets. The paper also contains a simple introduction to theory of ultrametric spaces and analysis on them.

Study on a neon cryogenic oscillating heat pipe with long heat transport distance

NASA Astrophysics Data System (ADS)

Liang, Qing; Li, Yi; Wang, Qiuliang

2018-06-01

An experimental study is carried out to study the heat transfer characteristics of a cryogenic oscillating heat pipe (OHP) with long heat transport distance. The OHP is made up of a capillary tube with an inner diameter of 1.0 mm and an outer diameter of 2.0 mm. The working fluid is neon, and the length of the adiabatic section is 480 mm. Tests are performed with the different heat inputs, liquid filling ratios and condenser temperature. For the cryogenic OHP with a liquid filling ratio of 30.7% at the condenser temperature of 28 K, the effective thermal conductivity is 3466-30,854 W/m K, and the maximum transfer power is 35.60 W. With the increment of the heat input, the effective thermal conductivity of the cryogenic OHP increases at the liquid filling ratios of 30.7% and 38.5%, while it first increases and then decreases at the liquid filling ratios of 15.2% and 23.3%. Moreover, the effective thermal conductivity increases with decreasing liquid filling ratio at the small heat input, and the maximum transfer power first increases and then decreases with increasing liquid filling ratio. Finally, it is found that the thermal performance of the cryogenic OHP can be improved by increasing the condenser temperature.

Waterspout as a special type of atmospheric aerosol dusty plasma

NASA Astrophysics Data System (ADS)

Rantsev-Kartinov, Valentin A.

2004-11-01

An analysis of databases of photographic images of oceanic surface revealed the presence of oceanic skeletal structures (OSS) [1] Rantsev-Kartinov V.A., Preprint . The OSSs presumably differ from the formerly found skeletal structures (SS) (Phys. Lett. A 306 (2002) 175) only by the fact that OSS are filled in with the closely packed blocks of a smaller size, up to thin, tens of microns-sized capillaries. The SSs in the Earth atmosphere were suggested [1] to be produced during atmospheric electricity activity by the volcanic-born dust. The fall-out of such SSs on the oceanic surface is a material source of OSS. Here we suggest that an OSS block [1] in the form of vertically oriented floating cylinder may be a stimulator of waterspout (WS). The main body of WS may be interpreted as a special type of atmospheric aerosol dusty plasma, and WS column - as a long-lived filament, being formed in the process of electric breakdown between the cloud and oceanic surface. The charged water drops aerosol may behave similar to microdust and lift upward to the cloud by the electrostatic force. With such a capillary&;electrostatic model of WS, it appears possible to interpret many effects related to WS.

Modeling the liquid filling in capillary well microplates for analyte preconcentration.

PubMed

Yu, Yang; Wang, Xuewei; Ng, Tuck Wah

2012-06-15

An attractive advantage of the capillary well microplate approach is the ability to conduct evaporative analyte preconcentration. We advance the use of hydrophobic materials for the wells which apart from reducing material loss through wetting also affords self entry into the well when the droplet size reduces below a critical value. Using Surface Evolver simulation without gravity, we find the critical diameters D(c) fitting very well with theoretical results. When simulating the critical diameters D(c)(G) with gravity included, the gravitational effect could only be ignored when the liquid volumes were small (difference of 5.7% with 5 μL of liquid), but not when the liquid volumes were large (differences of more than 22% with 50 μL of liquid). From this, we developed a modifying equation from a series of simulation results made to describe the gravitational effect. This modifying equation fitted the simulation results well in our simulation range (100°≤θ≤135° and 1 μL≤V≤200 μL). In simulating the condition of multiple wells underneath each droplet, we found that having more holes did not alter the critical diameters significantly. Consequently, the modifying relation should also generally express the critical diameter for multiple wells under a droplet. Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

Numerical Simulation of Passage of a Neutrophil through a Rectangular Channel with a Moderate Constriction

PubMed Central

Shirai, Atsushi; Masuda, Sunao

2013-01-01

The authors have previously presented a mathematical model to predict transit time of a neutrophil through an alveolar capillary segment which was modeled as an axisymmetric arc-shaped constriction settled in a cylindrical straight pipe to investigate the influence of entrance curvature of a capillary on passage of the cell. The axially asymmetric cross section of a capillary also influences the transit time because it requires three-dimensional deformation of a cell when it passes through the capillary and could lead to plasma leakage between the cell surface and the capillary wall. In this study, a rectangular channel was introduced, the side walls of which were moderately constricted, as a representative of axially asymmetric capillaries. Dependence of transit time of a neutrophil passing through the constriction on the constriction geometry, i.e., channel height, throat width and curvature radius of the constriction, was numerically investigated, the transit time being compared with that through the axisymmetric model. It was found that the transit time is dominated by the throat hydraulic diameter and curvature radius of the constriction and that the throat aspect ratio little affects the transit time with a certain limitation, indicating that if an appropriate curvature radius is chosen, such a rectangular channel model can be substituted for an axisymmetric capillary model having the same throat hydraulic diameter in terms of the transit time by choosing an appropriate curvature radius. Thus, microchannels fabricated by the photolithography technique, whose cross section is generally rectangular, are expected to be applicable to in vitro model experiments of neutrophil retention and passage in the alveolar capillaries. PMID:23527190

Development and demonstration of a water-window soft x-ray microscope using a Z-pinching capillary discharge source

NASA Astrophysics Data System (ADS)

Nawaz, M. F.; Jancarek, Alexandr; Nevrkla, Michal; Duda, Martin Jakub; Pina, Ladislav

2017-05-01

The development and demonstration of a soft X-ray (SXR) microscope, based on a Z-pinching capillary discharge source has been realized. The Z-pinching plasma acts as a source of SXR radiation. A ceramic capacitor bank is pulsed charged up to 80 kV, and discharged through a pre- ionized nitrogen filled ceramic capillary. The discharge current has an amplitude of 25 kA. Working within the water-window spectral region (λ = 2.88 nm), corresponding to the 1s2-1s2p quantum transition of helium-like nitrogen (N5+), the microscope has a potential in exploiting the natural contrast existing between the K-absorption edges of carbon and oxygen as the main constituents of biological materials, and hence imaging them with high spatial resolution. The SXR microscope uses the grazing incidence ellipsoidal condenser mirror for the illumination, and the Fresnel zone plate optics for the imaging of samples onto a BI-CCD camera. The half- pitch spatial resolution of 100 nm [1] was achieved, as demonstrated by the knife-edge test. In order to enhance the photon-flux at the sample plane, a new scheme for focusing the radiation, from multiple capillary sources has been investigated. Details about the source, and the construction of the microscope are presented and discussed.

Polymer microfluidic device replacing fluids using only capillary force

NASA Astrophysics Data System (ADS)

Chung, Kwang Hyo; Lee, Dae Sik; Yang, Haesik; Kim, Sung Jin; Pyo, Hyun Bong

2005-02-01

A novel polymer microfluidic device for self-wash using only capillary force is presented. A liquid filled in a reaction chamber is replaced by another liquid with no external actuation. All the fluidic actuations in the device is pre-programmed about time and sequence, and accomplished by capillary force naturally. Careful design is necessary for exact actions. The fluidic conduits were designed by the newly derived theoretical equations about the capillary stop pressure and flow time. Simulations using CFD-ACE+ were conducted to check the validity of theory and the performance of the chip. These analytic results were consistent with experimental ones. The chip was made of polymers for the purpose of single use and low price. It was fabricated by sealing the hot-embossed PMMA substrate with a PET film. For simpler fabrication, the chip was of a single height. The embossing master was produced from a nickel-electroplating on a SU8-patterned Ni-plate followed by CMP. The contact angles of liquids on substrates were manipulated through the mixing of surfactants, and the temporal variations were monitored for a more exact design. The real actuation steps in experiment revealed the stable performance of selfwash, and coincided well with the designed ones. The presented microfluidic method can be applicable to other LOCs of special purposes through simple modification. For example, array or serial types would be possible for multiple selfwashes.

Influence of local capillary trapping on containment system effectiveness

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bryant, Steven

2014-03-31

Immobilization of CO 2 injected into deep subsurface storage reservoirs is a critical component of risk assessment for geologic CO 2 storage (GCS). Local capillary trapping (LCT) is a recently established mode of immobilization that arises when CO 2 migrates due to buoyancy through heterogeneous storage reservoirs. This project sought to assess the amount and extent of LCT expected in storage formations under a range of injection conditions, and to confirm the persistence of LCT if the seal overlying the reservoir were to lose its integrity. Numerical simulation using commercial reservoir simulation software was conducted to assess the influence ofmore » injection. Laboratory experiments, modeling and numerical simulation were conducted to assess the effect of compromised seal integrity. Bench-scale (0.6 m by 0.6 m by 0.03 m) experiments with surrogate fluids provided the first empirical confirmation of the key concepts underlying LCT: accumulation of buoyant nonwetting phase at above residual saturations beneath capillary barriers in a variety of structures, which remains immobile under normal capillary pressure gradients. Immobilization of above-residual saturations is a critical distinction between LCT and the more familiar “residual saturation trapping.” To estimate the possible extent of LCT in a storage reservoir an algorithm was developed to identify all potential local traps, given the spatial distribution of capillary entry pressure in the reservoir. The algorithm assumes that the driving force for CO 2 migration can be represented as a single value of “critical capillary entry pressure” P c,entry crit, such that cells with capillary entry pressure greater/less than P c,entry crit act as barriers/potential traps during CO 2 migration. At intermediate values of P c,entry crit, the barrier regions become more laterally extensive in the reservoir, approaching a percolation threshold while non-barrier regions remain numerous. The maximum possible extent of LCT thus occurs at P c,entry crit near this threshold. Testing predictions of this simple algorithm against full-physics simulations of buoyancy-driven CO 2 migration support the concept of critical capillary entry pressure. However, further research is needed to determine whether a single value of critical capillary entry pressure always applies and how that value can be determined a priori. Simulations of injection into high-resolution (cells 0.3 m on a side) 2D and 3D heterogeneous domains show two characteristic behaviors. At small gravity numbers (vertical flow velocity much less than horizontal flow velocity) the CO 2 fills local traps as well as regions that would act as local barriers if CO 2 were moving only due to buoyancy. When injection ceases, the CO 2 migrates vertically to establish large saturations within local traps and residual saturation elsewhere. At large gravity numbers, the CO 2 invades a smaller portion of the perforated interval. Within this smaller swept zone the local barriers are not invaded, but local traps are filled to large saturation during injection and remain during post-injection gravity-driven migration. The small gravity number behavior is expected in the region within 100 m of a vertical injection well at anticipated rates of injection for commercial GCS. Simulations of leakage scenarios (through-going region of large permeability imposed in overlying seal) indicate that LCT persists (i.e. CO 2 remains held in a large fraction of the local iv traps) and the persistence is independent of injection rate during storage. Simulations of leakage for the limiting case of CO 2 migrating vertically from an areally extensive emplacement in the lower portion of a reservoir showed similar strong persistence of LCT. This research has two broad implications for GCS. The first is that LCT can retain a significant fraction of the CO 2 stored in a reservoir – above and beyond the residual saturation -- if the overlying seal were to fail. Thus frameworks for risk assessment should be extended to account for LCT. The second implication is that compared to pressure driven flow in reservoirs, CO 2 migration and trapping behave in a qualitatively different manner in heterogeneous reservoirs when buoyancy is the dominant driving force for flow. Thus simulations of GCS that neglect capillary heterogeneity will fail to capture important features of the CO 2 plume. While commercial reservoir simulation software can account for fine scale capillary heterogeneity, it has not been designed to work efficiently with such domains, and no simulators can handle fine-scale resolution throughout the reservoir. A possible way to upscale the migration and trapping is to apply an “effective residual saturation” to coarse-scale grids. While the extent of overall immobilization can be correlated in this way, all coarser grids failed to capture the distance traveled by the migrating CO 2 for large gravity number. Thus it remains unclear how best to account for LCT in the routine simulation work-flow that will be needed for large-scale GCS. Alternatives meriting investigation include streamline methods, reduced-physics proxies (e.g. particle tracking), and biased invasion percolation algorithms, which are based on precisely the capillary heterogeneity essential for LCT.« less

Effect of ultrasonic capillary dynamics on the mechanics of thermosonic ball bonding.

PubMed

Huang, Yan; Shah, Aashish; Mayer, Michael; Zhou, Norman Y; Persic, John

2010-01-01

Microelectronic wire bonding is an essential step in today's microchip production. It is used to weld (bond) microwires to metallized pads of integrated circuits using ultrasound with hundreds of thousands of vibration cycles. Thermosonic ball bonding is the most popular variant of the wire bonding process and frequently investigated using finite element (FE) models that simplify the ultrasonic dynamics of the process with static or quasistatic boundary conditions. In this study, the ultrasonic dynamics of the bonding tool (capillary), made from Al(2)O(3), is included in a FE model. For more accuracy of the FE model, the main material parameters are measured. The density of the capillary was measured to be rho(cap) = 3552 +/- 100 kg/m(3). The elastic modulus of the capillary, E(cap) = 389 +/- 11 GPa, is found by comparing an auxiliary FE model of the free vibrating capillary with measured values. A capillary "nodding effect" is identified and found to be essential when describing the ultrasonic vibration shape. A main FE model builds on these results and adds bonded ball, pad, chip, and die attach components. There is excellent agreement between the main model and the ultrasonic force measured at the interface on a test chip with stress microsensors. Bonded ball and underpad stress results are reported. When adjusted to the same ultrasonic force, a simplified model without ultrasonic dynamics and with an infinitely stiff capillary tip is substantially off target by -40% for the maximum underpad stress. The compliance of the capillary causes a substantial inclination effect at the bonding interface between wire and pad. This oscillating inclination effect massively influences the stress fields under the pad and is studied in more detail. For more accurate results, it is therefore recommended to include ultrasonic dynamics of the bonding tool in mechanical FE models of wire bonding.

Image-Based Modeling of Blood Flow and Oxygen Transfer in Feto-Placental Capillaries

PubMed Central

Brownbill, Paul; Janáček, Jiří; Jirkovská, Marie; Kubínová, Lucie; Chernyavsky, Igor L.; Jensen, Oliver E.

2016-01-01

During pregnancy, oxygen diffuses from maternal to fetal blood through villous trees in the placenta. In this paper, we simulate blood flow and oxygen transfer in feto-placental capillaries by converting three-dimensional representations of villous and capillary surfaces, reconstructed from confocal laser scanning microscopy, to finite-element meshes, and calculating values of vascular flow resistance and total oxygen transfer. The relationship between the total oxygen transfer rate and the pressure drop through the capillary is shown to be captured across a wide range of pressure drops by physical scaling laws and an upper bound on the oxygen transfer rate. A regression equation is introduced that can be used to estimate the oxygen transfer in a capillary using the vascular resistance. Two techniques for quantifying the effects of statistical variability, experimental uncertainty and pathological placental structure on the calculated properties are then introduced. First, scaling arguments are used to quantify the sensitivity of the model to uncertainties in the geometry and the parameters. Second, the effects of localized dilations in fetal capillaries are investigated using an idealized axisymmetric model, to quantify the possible effect of pathological placental structure on oxygen transfer. The model predicts how, for a fixed pressure drop through a capillary, oxygen transfer is maximized by an optimal width of the dilation. The results could explain the prevalence of fetal hypoxia in cases of delayed villous maturation, a pathology characterized by a lack of the vasculo-syncytial membranes often seen in conjunction with localized capillary dilations. PMID:27788214

The Relation Between Capillary Transit Times and Hemoglobin Saturation Heterogeneity. Part 1: Theoretical Models

PubMed Central

Lücker, Adrien; Secomb, Timothy W.; Weber, Bruno; Jenny, Patrick

2018-01-01

Capillary dysfunction impairs oxygen supply to parenchymal cells and often occurs in Alzheimer's disease, diabetes and aging. Disturbed capillary flow patterns have been shown to limit the efficacy of oxygen extraction and can be quantified using capillary transit time heterogeneity (CTH). However, the transit time of red blood cells (RBCs) through the microvasculature is not a direct measure of their capacity for oxygen delivery. Here we examine the relation between CTH and capillary outflow saturation heterogeneity (COSH), which is the heterogeneity of blood oxygen content at the venous end of capillaries. Models for the evolution of hemoglobin saturation heterogeneity (HSH) in capillary networks were developed and validated using a computational model with moving RBCs. Two representative situations were selected: a Krogh cylinder geometry with heterogeneous hemoglobin saturation (HS) at the inflow, and a parallel array of four capillaries. The heterogeneity of HS after converging capillary bifurcations was found to exponentially decrease with a time scale of 0.15–0.21 s due to diffusive interaction between RBCs. Similarly, the HS difference between parallel capillaries also drops exponentially with a time scale of 0.12–0.19 s. These decay times are substantially smaller than measured RBC transit times and only weakly depend on the distance between microvessels. This work shows that diffusive interaction strongly reduces COSH on a small spatial scale. Therefore, we conclude that CTH influences COSH yet does not determine it. The second part of this study will focus on simulations in microvascular networks from the rodent cerebral cortex. Actual estimates of COSH and CTH will then be given. PMID:29755365

The Relation Between Capillary Transit Times and Hemoglobin Saturation Heterogeneity. Part 1: Theoretical Models.

PubMed

Lücker, Adrien; Secomb, Timothy W; Weber, Bruno; Jenny, Patrick

2018-01-01

Capillary dysfunction impairs oxygen supply to parenchymal cells and often occurs in Alzheimer's disease, diabetes and aging. Disturbed capillary flow patterns have been shown to limit the efficacy of oxygen extraction and can be quantified using capillary transit time heterogeneity (CTH). However, the transit time of red blood cells (RBCs) through the microvasculature is not a direct measure of their capacity for oxygen delivery. Here we examine the relation between CTH and capillary outflow saturation heterogeneity (COSH), which is the heterogeneity of blood oxygen content at the venous end of capillaries. Models for the evolution of hemoglobin saturation heterogeneity (HSH) in capillary networks were developed and validated using a computational model with moving RBCs. Two representative situations were selected: a Krogh cylinder geometry with heterogeneous hemoglobin saturation (HS) at the inflow, and a parallel array of four capillaries. The heterogeneity of HS after converging capillary bifurcations was found to exponentially decrease with a time scale of 0.15-0.21 s due to diffusive interaction between RBCs. Similarly, the HS difference between parallel capillaries also drops exponentially with a time scale of 0.12-0.19 s. These decay times are substantially smaller than measured RBC transit times and only weakly depend on the distance between microvessels. This work shows that diffusive interaction strongly reduces COSH on a small spatial scale. Therefore, we conclude that CTH influences COSH yet does not determine it. The second part of this study will focus on simulations in microvascular networks from the rodent cerebral cortex. Actual estimates of COSH and CTH will then be given.

A Simple Double-Source Model for Interference of Capillaries

ERIC Educational Resources Information Center

Hou, Zhibo; Zhao, Xiaohong; Xiao, Jinghua

2012-01-01

A simple but physically intuitive double-source model is proposed to explain the interferogram of a laser-capillary system, where two effective virtual sources are used to describe the rays reflected by and transmitted through the capillary. The locations of the two virtual sources are functions of the observing positions on the target screen. An…

A theoretical model for optical oximetry at the capillary-level by optical coherence tomography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Liu, Rongrong; Spicer, Graham; Chen, Siyu; Zhang, Hao F.; Yi, Ji; Backman, Vadim

2017-02-01

Oxygen saturation (sO2) of RBCs in capillaries can indirectly assess local tissue oxygenation and metabolic function. For example, the altered retinal oxygenation in diabetic retinopathy and local hypoxia during tumor development in cancer are reflected by abnormal sO2 of local capillary networks. However, it is far from clear whether accurate label-free optical oximetry (i.e. measuring hemoglobin sO2) is feasible from dispersed red blood cells (RBCs) at the single-capillary level. The sO2-dependent hemoglobin absorption contrast present in optical scattering signal is complicated by geometry-dependent scattering from RBCs. Here we provide a theoretical model to calculate the backscattering spectra of single RBCs based on the first-order Born approximation, considering the orientation, size variation, and deformation of RBCs. We show that the oscillatory spectral behavior of RBC geometries is smoothed by variations in cell size and orientation, resulting in clear sO2-dependent spectral contrast. In addition, this spectral contrast persists with different deformations of RBCs, allowing the sO2 of individual RBCs in capillaries to be characterized. The theoretical model is verified by Mie theory and experiments using visible light optical coherence tomography (vis-OCT). Thus, this study shows for the first time the feasibility of, and provides a theoretical model for, label-free optical oximetry at the single-capillary level by backscattering-based imaging modalities, challenging the popular view that such measurements are impossible at the single-capillary level. This is promising for in vivo backscattering-based optical oximetry at the single-capillary level, to measure local capillary sO2 for early diagnosis, progression monitoring, and treatment evaluation of diabetic retinopathy and cancer.

Investigation of pressure drop in capillary tube for mixed refrigerant Joule-Thomson cryocooler

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ardhapurkar, P. M.; Sridharan, Arunkumar; Atrey, M. D.

2014-01-29

A capillary tube is commonly used in small capacity refrigeration and air-conditioning systems. It is also a preferred expansion device in mixed refrigerant Joule-Thomson (MR J-T) cryocoolers, since it is inexpensive and simple in configuration. However, the flow inside a capillary tube is complex, since flashing process that occurs in case of refrigeration and air-conditioning systems is metastable. A mixture of refrigerants such as nitrogen, methane, ethane, propane and iso-butane expands below its inversion temperature in the capillary tube of MR J-T cryocooler and reaches cryogenic temperature. The mass flow rate of refrigerant mixture circulating through capillary tube depends onmore » the pressure difference across it. There are many empirical correlations which predict pressure drop across the capillary tube. However, they have not been tested for refrigerant mixtures and for operating conditions of the cryocooler. The present paper assesses the existing empirical correlations for predicting overall pressure drop across the capillary tube for the MR J-T cryocooler. The empirical correlations refer to homogeneous as well as separated flow models. Experiments are carried out to measure the overall pressure drop across the capillary tube for the cooler. Three different compositions of refrigerant mixture are used to study the pressure drop variations. The predicted overall pressure drop across the capillary tube is compared with the experimentally obtained value. The predictions obtained using homogeneous model show better match with the experimental results compared to separated flow models.« less

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

PubMed Central

Deng, Yongbo; Fan, Jianhua; Zhou, Song; Zhou, Teng; Wu, Junfeng; Li, Yin; Liu, Zhenyu; Xuan, Ming; Wu, Yihui

2014-01-01

Based on the Euler force induced by the acceleration of compact disk (CD)-like microfluidic chip, this paper presents a novel actuation mechanism for siphon valving. At the preliminary stage of acceleration, the Euler force in the tangential direction of CD-like chip takes the primary place compared with the centrifugal force to function as the actuation of the flow, which fills the siphon and actuates the siphon valving. The Euler force actuation mechanism is demonstrated by the numerical solution of the phase-field based mathematical model for the flow in siphon valve. In addition, experimental validation is implemented in the polymethylmethacrylate-based CD-like microfluidic chip manufactured using CO2 laser engraving technique. To prove the application of the proposed Euler force actuation mechanism, whole blood separation and plasma extraction has been conducted using the Euler force actuated siphon valving. The newly introduced actuation mechanism overcomes the dependence on hydrophilic capillary filling of siphon by avoiding external manipulation or surface treatments of polymeric material. The sacrifice for highly integrated processing in pneumatic pumping technique is also prevented by excluding the volume-occupied compressed air chamber. PMID:24753736

Numerical simulation of flow for viscoelastic neutrophil models in a rectangular capillary network: effects of capillary shape and cell stiffness on transit time.

PubMed

Shirai, Atsushi; Fujita, Ryo; Hayase, Toshiyuki

2007-01-01

The concentration of neutrophils in the pulmonary microvasculature is higher than in large systemic vessels. It is thought that the high concentration of neutrophils facilitates their effective recruitment to sites of inflammation. Thus, in order to understand the role of neutrophils in the immune system, it is important to clarify their flow characteristics in the pulmonary microvasculature. In a previous study, we developed a model to simulate the flow of neutrophils in a capillary network, in which the cells were modeled as spheres of a Maxwell material with a cortical tension and the capillary segments were modeled as arc-shaped constrictions in straight pipes. In the present paper, the flow of neutrophils in a simplified alveolar capillary network model is investigated for various constriction shapes and cell stiffnesses. Finally, it is shown that both the coefficient of variation of the transit time of the cells, which is the standard deviation divided by the mean transit time, and the mean transit time increase as the capillary segments become steep or tight, or when the cells become hard. The mean value of the transit time exceeds the median for all of the conditions that occur in real lungs, although the difference between them is small.

Circumventing Imprecise Geometric Information and Development of a Unified Modeling Technique for Various Flow Regimes in Capillary Tubes

NASA Astrophysics Data System (ADS)

Abbasi, Bahman

2012-11-01

Owing to their manufacturability and reliability, capillary tubes are the most common expansion devices in household refrigerators. Therefore, investigating flow properties in the capillary tubes is of immense appeal in the said business. The models to predict pressure drop in two-phase internal flows invariably rely upon highly precise geometric information. The manner in which capillary tubes are manufactured makes them highly susceptible to geometric imprecisions, which renders geometry-based models unreliable to the point of obsoleteness. Aware of the issue, manufacturers categorize capillary tubes based on Nitrogen flow rate through them. This categorization method presents an opportunity to substitute geometric details with Nitrogen flow data as the basis for customized models. The simulation tools developed by implementation of this technique have the singular advantage of being applicable across flow regimes. Thus the error-prone process of identifying compatible correlations is eliminated. Equally importantly, compressibility and chocking effects can be incorporated in the same model. The outcome is a standalone correlation that provides accurate predictions, regardless of any particular fluid or flow regime. Thereby, exploratory investigations for capillary tube design and optimization are greatly simplified. Bahman Abbasi, Ph.D., is Lead Advanced Systems Engineer at General Electric Appliances in Louisville, KY. He conducts research projects across disciplines in the household refrigeration industry.

Controlling retention, selectivity and magnitude of EOF by segmented monolithic columns consisting of octadecyl and naphthyl monolithic segments--applications to RP-CEC of both neutral and charged solutes.

PubMed

Karenga, Samuel; El Rassi, Ziad

2011-04-01

Monolithic capillaries made of two adjoining segments each filled with a different monolith were introduced for the control and manipulation of the electroosmotic flow (EOF), retention and selectivity in reversed phase-capillary electrochromatography (RP-CEC). These columns were called segmented monolithic columns (SMCs) where one segment was filled with a naphthyl methacrylate monolith (NMM) to provide hydrophobic and π-interactions, while the other segment was filled with an octadecyl acrylate monolith (ODM) to provide solely hydrophobic interaction. The ODM segment not only provided hydrophobic interactions but also functioned as the EOF accelerator segment. The average EOF of the SMC increased linearly with increasing the fractional length of the ODM segment. The neutral SMC provided a convenient way for tuning EOF, selectivity and retention in the absence of annoying electrostatic interactions and irreversible solute adsorption. The SMCs allowed the separation of a wide range of neutral solutes including polycyclic aromatic hydrocarbons (PAHs) that are difficult to separate using conventional alkyl-bonded stationary phases. In all cases, the k' of a given solute was a linear function of the fractional length of the ODM or NMM segment in the SMCs, thus facilitating the tailoring of a given SMC to solve a given separation problem. At some ODM fractional length, the fabricated SMC allowed the separation of charged solutes such as peptides and proteins that could not otherwise be achieved on a monolithic column made from NMM as an isotropic stationary phase due to the lower EOF exhibited by this monolith. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A Gibbs point field model for the spatial pattern of coronary capillaries

NASA Astrophysics Data System (ADS)

Karch, R.; Neumann, M.; Neumann, F.; Ullrich, R.; Neumüller, J.; Schreiner, W.

2006-09-01

We propose a Gibbs point field model for the pattern of coronary capillaries in transverse histologic sections from human hearts, based on the physiology of oxygen supply from capillaries to tissue. To specify the potential energy function of the Gibbs point field, we draw on an analogy between the equation of steady-state oxygen diffusion from an array of parallel capillaries to the surrounding tissue and Poisson's equation for the electrostatic potential of a two-dimensional distribution of identical point charges. The influence of factors other than diffusion is treated as a thermal disturbance. On this basis, we arrive at the well-known two-dimensional one-component plasma, a system of identical point charges exhibiting a weak (logarithmic) repulsive interaction that is completely characterized by a single dimensionless parameter. By variation of this parameter, the model is able to reproduce many characteristics of real capillary patterns.

A coupling modulation model of capillary waves from gravity waves: Theoretical analysis and experimental validation

NASA Astrophysics Data System (ADS)

Chen, Pengzhen; Wang, Xiaoqing; Liu, Li; Chong, Jinsong

2016-06-01

According to Bragg theory, capillary waves are the predominant scatterers of high-frequency band (such as Ka-band) microwave radiation from the surface of the ocean. Therefore, understanding the modulation mechanism of capillary waves is an important foundation for interpreting high-frequency microwave remote sensing images of the surface of the sea. In our experiments, we discovered that modulations of capillary waves are significantly larger than the values predicted by the classical theory. Further, analysis shows that the difference in restoring force results in an inflection point while the phase velocity changes from gravity waves region to capillary waves region, and this results in the capillary waves being able to resonate with gravity waves when the phase velocity of the gravity waves is equal to the group velocity of the capillary waves. Consequently, we propose a coupling modulation model in which the current modulates the capillary wave indirectly by modulating the resonant gravity waves, and the modulation of the former is approximated by that of the latter. This model very effectively explains the results discovered in our experiments. Further, based on Bragg scattering theory and this coupling modulation model, we simulate the modulation of normalized radar cross section (NRCS) of typical internal waves and show that the high-frequency bands are superior to the low-frequency bands because of their greater modulation of NRCS and better radiometric resolution. This result provides new support for choice of radar band for observation of wave-current modulation oceanic phenomena such as internal waves, fronts, and shears.

A novel method of creation capillary structures in metal parts based on using selective laser melting methid of 3D printing technology and surface roughness

NASA Astrophysics Data System (ADS)

Ivanov, Roman A.; Melkikh, Alexey V.

2017-09-01

It has been experimentally proved that it is possible to produce a metal capillary structure with significant capillary action and free shape configuration using selective laser melting. Capillaries are created by dividing the solid detail volume into micro-sized parallel walls with roughness as a result of SLM 3D printing. Experiments are conducted on aluminum powder with particle size in the range of 10-40 µm (,) and distances in 3D model between surfaces incapillary generation zone in the range of 50-200 µm. It is showed that products produced from model with 100 µm gaps have the greatest efficiency of fluid lifting as a result of obtaining stable arrays of capillaries of 20-40 µm in size. Change in the direction of (growing) printingthe product doesn't significantly influence on capillary geometry, but it affects on safety of the structure.

Microgravity Investigation of Capillary Driven Imbibition

NASA Astrophysics Data System (ADS)

Dushin, V. R.; Nikitin, V. F.; Smirnov, N. N.; Skryleva, E. I.; Tyurenkova, V. V.

2018-05-01

The goal of the present paper is to investigate the capillary driven filtration in porous media under microgravity conditions. New mathematical model that allows taking into account the blurring of the front due to the instability of the displacement that is developing at the front is proposed. The constants in the mathematical model were selected on the basis of the experimental data on imbibition into unsaturated porous media under microgravity conditions. The flow under the action of a combination of capillary forces and a constant pressure drop or a constant flux is considered. The effect of capillary forces and the type of wettability of the medium on the displacement process is studied. A criterion in which case the capillary effects are insignificant and can be neglected is established.

Mathematical Model of Bubble Sloshing Dynamics for Cryogenic Liquid Helium in Orbital Spacecraft Dewar Container

NASA Technical Reports Server (NTRS)

Hung, R. J.; Pan, H. L.

1995-01-01

A generalized mathematical model is investigated of sloshing dynamics for dewar containers, partially filled with a liquid of cryogenic superfluid helium 2, driven by both gravity gradient and jitter accelerations applicable to two types of scientific spacecrafts, which are eligible to carry out spinning motion and/or slew motion to perform scientific observations during normal spacecraft operation. Two examples are given for the Gravity Probe-B (GP-B) with spinning motion, and the Advanced X-Ray Astrophysics Facility-Spectroscopy (AXAF-S) with slew motion, which are responsible for the sloshing dynamics. Explicit mathematical expressions for the modelling of sloshing dynamics to cover these forces acting on the spacecraft fluid systems are derived. The numerical computation of sloshing dynamics will be based on the noninertial frame spacecraft bound coordinate, and we will solve the time-dependent three-dimensional formulations of partial differential equations subject to initial and boundary conditions. Explicit mathematical expressions of boundary conditions lo cover capillary force effects on the liquid-vapor interface in microgravity environments are also derived. Results of the simulations of the mathematical model are illustrated.

Living cell manipulation, manageable sampling, and shotgun picoliter electrospray mass spectrometry for profiling metabolites.

PubMed

Gholipour, Yousef; Erra-Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi

2013-02-01

A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions. Copyright © 2012 Elsevier Inc. All rights reserved.

Nanokit for single-cell electrochemical analyses.

PubMed

Pan, Rongrong; Xu, Mingchen; Jiang, Dechen; Burgess, Jame D; Chen, Hong-Yuan

2016-10-11

The development of more intricate devices for the analysis of small molecules and protein activity in single cells would advance our knowledge of cellular heterogeneity and signaling cascades. Therefore, in this study, a nanokit was produced by filling a nanometer-sized capillary with a ring electrode at the tip with components from traditional kits, which could be egressed outside the capillary by electrochemical pumping. At the tip, femtoliter amounts of the kit components were reacted with the analyte to generate hydrogen peroxide for the electrochemical measurement by the ring electrode. Taking advantage of the nanotip and small volume injection, the nanokit was easily inserted into a single cell to determine the intracellular glucose levels and sphingomyelinase (SMase) activity, which had rarely been achieved. High cellular heterogeneities of these two molecules were observed, showing the significance of the nanokit. Compared with the current methods that use a complicated structural design or surface functionalization for the recognition of the analytes, the nanokit has adapted features of the well-established kits and integrated the kit components and detector in one nanometer-sized capillary, which provides a specific device to characterize the reactivity and concentrations of cellular compounds in single cells.

Mechanism of Kinetically Controlled Capillary Condensation in Nanopores: A Combined Experimental and Monte Carlo Approach.

PubMed

Hiratsuka, Tatsumasa; Tanaka, Hideki; Miyahara, Minoru T

2017-01-24

We find the rule of capillary condensation from the metastable state in nanoscale pores based on the transition state theory. The conventional thermodynamic theories cannot achieve it because the metastable capillary condensation inherently includes an activated process. We thus compute argon adsorption isotherms on cylindrical pore models and atomistic silica pore models mimicking the MCM-41 materials by the grand canonical Monte Carlo and the gauge cell Monte Carlo methods and evaluate the rate constant for the capillary condensation by the transition state theory. The results reveal that the rate drastically increases with a small increase in the chemical potential of the system, and the metastable capillary condensation occurs for any mesopores when the rate constant reaches a universal critical value. Furthermore, a careful comparison between experimental adsorption isotherms and the simulated ones on the atomistic silica pore models reveals that the rate constant of the real system also has a universal value. With this finding, we can successfully estimate the experimental capillary condensation pressure over a wide range of temperatures and pore sizes by simply applying the critical rate constant.

Performance model of a recirculating stack nickel hydrogen cell

NASA Technical Reports Server (NTRS)

Zimmerman, Albert H.

1994-01-01

A theoretical model of the nickel hydrogen battery cell has been utilized to describe the chemical and physical changes during charge and overcharge in a recirculating stack nickel hydrogen cell. In particular, the movement of gas and electrolyte have been examined as a function of the amount of electrolyte put into the cell stack during cell activation, and as a function of flooding in regions of the gas screen in this cell design. Additionally, a two-dimensional variation on this model has been utilized to describe the effects of non-uniform loading in the nickel-electrode on the movement of gas and electrolyte within the recirculating stack nickel hydrogen cell. The type of nonuniform loading that has been examined here is that associated with higher than average loading near the surface of the sintered nickel electrode, a condition present to some degree in many nickel electrodes made by electrochemical impregnation methods. The effects of high surface loading were examined primarily under conditions of overcharge, since the movement of gas and electrolyte in the overcharging condition was typically where the greatest effects of non-uniform loading were found. The results indicate that significant changes in the capillary forces between cell components occur as the percentage of free volume in the stack filled by electrolyte becomes very high. These changes create large gradients in gas-filled space and oxygen concentrations near the boundary between the separator and the hydrogen electrode when the electrolyte fill is much greater than about 95 percent of the stack free volume. At lower electrolyte fill levels, these gaseous and electrolyte gradients become less extreme, and shift through the separator towards the nickel electrode. Similarly, flooding of areas in the gas screen cause higher concentrations of oxygen gas to approach the platinum/hydrogen electrode that is opposite the back side of the nickel electrode. These results illustrate the need for appropriate pore size distributions, and the maintenance of both convective electrolyte and gas flow paths through the stack, if the recirculating stack nickel hydrogen cell design is to work properly.

A boundary-integral representation for biphasic mixture theory, with application to the post-capillary glycocalyx

PubMed Central

Sumets, P. P.; Cater, J. E.; Long, D. S.; Clarke, R. J.

2015-01-01

We describe a new boundary-integral representation for biphasic mixture theory, which allows us to efficiently solve certain elastohydrodynamic–mobility problems using boundary element methods. We apply this formulation to model the motion of a rigid particle through a microtube which has non-uniform wall shape, is filled with a viscous Newtonian fluid, and is lined with a thin poroelastic layer. This is relevant to scenarios such as the transport of small rigid cells (such as neutrophils) through microvessels that are lined with an endothelial glycocalyx layer (EGL). In this context, we examine the impact of geometry upon some recently reported phenomena, including the creation of viscous eddies, fluid flux into the EGL, as well as the role of the EGL in transmitting mechanical signals to the underlying endothelial cells. PMID:26345494

Immediate Dose-Response Effect of High-Energy Versus Low-Energy Extracorporeal Shock Wave Therapy on Cutaneous Microcirculation.

PubMed

Kraemer, Robert; Sorg, Heiko; Forstmeier, Vinzent; Knobloch, Karsten; Liodaki, Eirini; Stang, Felix Hagen; Mailaender, Peter; Kisch, Tobias

2016-12-01

Elucidation of the precise mechanisms and therapeutic options of extracorporeal shock wave therapy (ESWT) is only at the beginning. Although immediate real-time effects of ESWT on cutaneous hemodynamics have recently been described, the dose response to different ESWT energies in cutaneous microcirculation has never been examined. Thirty-nine Sprague-Dawley rats were randomly assigned to three groups that received either focused high-energy shock waves (group A: total of 1000 impulses, 10 J) to the lower leg of the hind limb, focused low-energy shock waves (group B: total of 300 impulses, 1 J) or placebo shock wave treatment (group C: 0 impulses, 0 J) using a multimodality shock wave delivery system (Duolith SD-1 T-Top, Storz Medical, Tägerwilen, Switzerland). Immediate microcirculatory effects were assessed with the O2C (oxygen to see) system (LEA Medizintechnik, Giessen, Germany) before and for 20 min after application of ESWT. Cutaneous tissue oxygen saturation increased significantly higher after high-energy ESWT than after low-energy and placebo ESWT (A: 29.4% vs. B: 17.3% vs. C: 3.3%; p = 0.003). Capillary blood velocity was significantly higher after high-energy ESWT and lower after low-energy ESWT versus placebo ESWT (group A: 17.8% vs. group B: -22.1% vs. group C: -5.0%, p = 0.045). Post-capillary venous filling pressure was significantly enhanced in the high-energy ESWT group in contrast to the low-energy ESWT and placebo groups (group A: 25% vs. group B: 2% vs. group C: -4%, p = 0.001). Both high-energy and low-energy ESWT affect cutaneous hemodynamics in a standard rat model. High-energy ESWT significantly increases parameters of cutaneous microcirculation immediately after application, resulting in higher tissue oxygen saturation, venous filling pressure and blood velocity, which suggests higher tissue perfusion with enhanced oxygen saturation, in contrast to low-energy as well as placebo ESWT. Low-energy ESWT also increased tissue oxygen saturation, albeit to a lower extent, and decreases both blood velocity and venous filling pressure. Low-energy ESWT reduced tissue perfusion, but improved oxygen saturation immediately after the application. Copyright © 2016 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip.

PubMed

Liu, Vincent; Song, Yong-Ak; Han, Jongyoon

2010-06-07

In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchannels with an ion-selective material such as Nafion resin; subsequent curing results in a high aspect-ratio membrane for use in electrokinetic sample preconcentration. To demonstrate the concentration performance of this high-aspect-ratio, ion-selective membrane, we integrated the preconcentrator with a surface-based immunoassay for R-Phycoerythrin (RPE). Using a 1x PBS buffer system, the preconcentrator-enhanced immunoassay showed an approximately 100x improvement in sensitivity within 30 min. This is the first time that an electrokinetic microfluidic preconcentrator based on ion concentration polarization (ICP) has been used in high ionic strength buffer solutions to enhance the sensitivity of a surface-based immunoassay.

Magnetic properties of permalloy wires in vycor capillaries

NASA Astrophysics Data System (ADS)

Lubitz, P.; Ayers, J. D.; Davis, A.

1991-11-01

Thin wires of NiFe alloys with compositions near 80% Ni were prepared by melting the alloy in vycor tubes and drawing fibers from the softened glass. The resulting fibers consist of relatively thick-walled vycor capillaries containing permalloy wires filling a few percent of the volume. The wires are continuous over considerable lengths, uniform in circular cross section, nearly free of contact with the walls and can be drawn to have diameters less than 1 μm. Their magnetic properties are generally similar to bulk permalloy, but show a variety of magnetic switching behaviors for fields along the wire axis, depending on composition, wire diameter, and thermal history. As pulled, the wires can show sharp switching, reversible rotation or mixed behavior. This method can produce NiFe alloy wires suitable for use in applications as sensor, memory or inductive elements; other alloys, such as supermalloy and sendust, also can be fabricated as fine wires by this method.

Properties of Cement Mortar Produced from Mixed Waste Materials with Pozzolanic Characteristics.

PubMed

Yen, Chi-Liang; Tseng, Dyi-Hwa; Wu, Yue-Ze

2012-07-01

Waste materials with pozzolanic characteristics, such as sewage sludge ash (SSA), coal combustion fly ash (FA), and granulated blast furnace slag (GBS), were reused as partial cement replacements for making cement mortar in this study. Experimental results revealed that with dual replacement of cement by SSA and GBS and triple replacement by SSA, FA, and GBS at 50% of total cement replacement, the compressive strength (Sc) of the blended cement mortars at 56 days was 93.7% and 92.9% of the control cement mortar, respectively. GBS had the highest strength activity index value and could produce large amounts of CaO to enhance the pozzolanic activity of SSA/FA and form calcium silicate hydrate gels to fill the capillary pores of the cement mortar. Consequently, the Sc development of cement mortar with GBS replacement was better than that without GBS, and the total pore volume of blended cement mortars with GBS/SSA replacement was less than that with FA/SSA replacement. In the cement mortar with modified SSA and GBS at 70% of total cement replacement, the Sc at 56 days was 92.4% of the control mortar. Modifying the content of calcium in SSA also increased its pozzolanic reaction. CaCl(2) accelerated the pozzolanic activity of SSA better than lime did. Moreover, blending cement mortars with GBS/SSA replacement could generate more monosulfoaluminate to fill capillary pores.

Properties of Cement Mortar Produced from Mixed Waste Materials with Pozzolanic Characteristics

PubMed Central

Yen, Chi-Liang; Tseng, Dyi-Hwa; Wu, Yue-Ze

2012-01-01

Abstract Waste materials with pozzolanic characteristics, such as sewage sludge ash (SSA), coal combustion fly ash (FA), and granulated blast furnace slag (GBS), were reused as partial cement replacements for making cement mortar in this study. Experimental results revealed that with dual replacement of cement by SSA and GBS and triple replacement by SSA, FA, and GBS at 50% of total cement replacement, the compressive strength (Sc) of the blended cement mortars at 56 days was 93.7% and 92.9% of the control cement mortar, respectively. GBS had the highest strength activity index value and could produce large amounts of CaO to enhance the pozzolanic activity of SSA/FA and form calcium silicate hydrate gels to fill the capillary pores of the cement mortar. Consequently, the Sc development of cement mortar with GBS replacement was better than that without GBS, and the total pore volume of blended cement mortars with GBS/SSA replacement was less than that with FA/SSA replacement. In the cement mortar with modified SSA and GBS at 70% of total cement replacement, the Sc at 56 days was 92.4% of the control mortar. Modifying the content of calcium in SSA also increased its pozzolanic reaction. CaCl2 accelerated the pozzolanic activity of SSA better than lime did. Moreover, blending cement mortars with GBS/SSA replacement could generate more monosulfoaluminate to fill capillary pores. PMID:22783062

Wettability impact on supercritical CO2 capillary trapping: Pore-scale visualization and quantification

NASA Astrophysics Data System (ADS)

Hu, Ran; Wan, Jiamin; Kim, Yongman; Tokunaga, Tetsu K.

2017-08-01

How the wettability of pore surfaces affects supercritical (sc) CO2 capillary trapping in geologic carbon sequestration (GCS) is not well understood, and available evidence appears inconsistent. Using a high-pressure micromodel-microscopy system with image analysis, we studied the impact of wettability on scCO2 capillary trapping during short-term brine flooding (80 s, 8-667 pore volumes). Experiments on brine displacing scCO2 were conducted at 8.5 MPa and 45°C in water-wet (static contact angle θ = 20° ± 8°) and intermediate-wet (θ = 94° ± 13°) homogeneous micromodels under four different flow rates (capillary number Ca ranging from 9 × 10-6 to 8 × 10-4) with a total of eight conditions (four replicates for each). Brine invasion processes were recorded and statistical analysis was performed for over 2000 images of scCO2 saturations, and scCO2 cluster characteristics. The trapped scCO2 saturation under intermediate-wet conditions is 15% higher than under water-wet conditions under the slowest flow rate (Ca ˜ 9 × 10-6). Based on the visualization and scCO2 cluster analysis, we show that the scCO2 trapping process in our micromodels is governed by bypass trapping that is enhanced by the larger contact angle. Smaller contact angles enhance cooperative pore filling and widen brine fingers (or channels), leading to smaller volumes of scCO2 being bypassed. Increased flow rates suppress this wettability effect.

Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates

PubMed Central

Chen, Jin; Venugopal, Vivek; Intes, Xavier

2011-01-01

Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full fluorescence time measurements, a light propagation model that accurately simulates weakly diffused and multiple scattered photons is required. In this article, we derive a computationally efficient Monte Carlo based method to compute time-gated fluorescence Jacobians for the simultaneous imaging of two fluorophores with lifetime contrast. The Monte Carlo based formulation is validated on a synthetic murine model simulating the uptake in the kidneys of two distinct fluorophores with lifetime contrast. Experimentally, the method is validated using capillaries filled with 2.5nmol of ICG and IRDye™800CW respectively embedded in a diffuse media mimicking the average optical properties of mice. Combining multiple time gates in one inverse problem allows the simultaneous reconstruction of multiple fluorophores with increased resolution and minimal crosstalk using the proposed formulation. PMID:21483610

TOPICAL REVIEW Recent developments in inorganically filled carbon nanotubes: successes and challenges

NASA Astrophysics Data System (ADS)

Gautam, Ujjal K.; Costa, Pedro M. F. J.; Bando, Yoshio; Fang, Xiaosheng; Li, Liang; Imura, Masataka; Golberg, Dmitri

2010-10-01

Carbon nanotubes (CNTs) are a unique class of nanomaterials that can be imagined as rolled graphene sheets. The inner hollow of a CNT provides an extremely small, one-dimensional space for storage of materials. In the last decade, enormous effort has been spent to produce filled CNTs that combine the properties of both the host CNT and the guest filling material. CNTs filled with various inorganic materials such as metals, alloys, semiconductors and insulators have been obtained using different synthesis approaches including capillary filling and chemical vapor deposition. Recently, several potential applications have emerged for these materials, such as the measurement of temperature at the nanoscale, nano-spot welding, and the storage and delivery of extremely small quantities of materials. A clear distinction between this class of materials and other nanostructures is the existence of an enormous interfacial area between the CNT and the filling matter. Theoretical investigations have shown that the lattice mismatch and strong exchange interaction of CNTs with the guest material across the interface should result in reordering of the guest crystal structure and passivation of the surface dangling bonds and thus yielding new and interesting physical properties. Despite preliminary successes, there remain many challenges in realizing applications of CNTs filled with inorganic materials, such as a comprehensive understanding of their growth and physical properties and control of their structural parameters. In this article, we overview research on filled CNT nanomaterials with special emphasis on recent progress and key achievements. We also discuss the future scope and the key challenges emerging out of a decade of intensive research on these fascinating materials.

Left atrial strain: a new parameter for assessment of left ventricular filling pressure.

PubMed

Cameli, Matteo; Mandoli, Giulia Elena; Loiacono, Ferdinando; Dini, Frank Lloyd; Henein, Michael; Mondillo, Sergio

2016-01-01

In order to obtain accurate diagnosis, treatment and prognostication in many cardiac conditions, there is a need for assessment of left ventricular (LV) filling pressure. While systole depends on ejection function of LV, diastole and its disturbances influence filling function and pressures. The commonest condition that represents the latter is heart failure with preserved ejection fraction in which LV ejection is maintained, but diastole is disturbed and hence filling pressures are raised. Significant diastolic dysfunction results in raised LV end-diastolic pressure, mean left atrial (LA) pressure and pulmonary capillary wedge pressure, all referred to as LV filling pressures. Left and right heart catheterization has traditionally been used as the gold standard investigation for assessing these pressures. More recently, Doppler echocardiography has taken over such application because of its noninvasive nature and for being patient friendly. A number of indices are used to achieve accurate assessment of filling pressures including: LV pulsed-wave filling velocities (E/A ratio, E wave deceleration time), pulmonary venous flow (S wave and D wave), tissue Doppler imaging (E' wave and E/E' ratio) and LA volume index. LA longitudinal strain derived from speckle tracking echocardiography (STE) is also sensitive in estimating intracavitary pressures. It is angle-independent, thus overcomes Doppler limitations and provides highly reproducible measures of LA deformation. This review examines the application of various Doppler echocardiographic techniques in assessing LV filling pressures, in particular the emerging role of STE in assessing LA pressures in various conditions, e.g., HF, arterial hypertension and atrial fibrillation.

Comparison of HPLC and CE for the analysis of dichlorprop in a case of intoxication.

PubMed

West, A; Frost, M; Köhler, H

1997-01-01

A 49-year-old white male was found lying unconscious at home. He had vomited, his mouth was filled with a white foam and a pungent odour filled the room. After emergency treatment blood, urine and stomach contents were screened for drugs after acid and alkaline extraction with subsequent derivatisation and GC-MS analysis. Large quantites of 2,4-dichlorophenoxypropionic acid (dichlorprop, 2,4-DP) were found in all acid extracts. The man died 3 h later in hospital. Body fluids and tissues obtained at autopsy were analysed for 2,4-DP by high-performance liquid chromatography and capillary electrophoresis. The concentrations of 2,4-DP in cardiac blood, stomach contents, bile, liver, spleen, kidney and brain found by both methods were very similar.

Depicting mass flow rate of R134a /LPG refrigerant through straight and helical coiled adiabatic capillary tubes of vapor compression refrigeration system using artificial neural network approach

NASA Astrophysics Data System (ADS)

Gill, Jatinder; Singh, Jagdev

2018-07-01

In this work, an experimental investigation is carried out with R134a and LPG refrigerant mixture for depicting mass flow rate through straight and helical coil adiabatic capillary tubes in a vapor compression refrigeration system. Various experiments were conducted under steady-state conditions, by changing capillary tube length, inner diameter, coil diameter and degree of subcooling. The results showed that mass flow rate through helical coil capillary tube was found lower than straight capillary tube by about 5-16%. Dimensionless correlation and Artificial Neural Network (ANN) models were developed to predict mass flow rate. It was found that dimensionless correlation and ANN model predictions agreed well with experimental results and brought out an absolute fraction of variance of 0.961 and 0.988, root mean square error of 0.489 and 0.275 and mean absolute percentage error of 4.75% and 2.31% respectively. The results suggested that ANN model shows better statistical prediction than dimensionless correlation model.

Influence of Capillary Force and Buoyancy on CO2 Migration During CO2 Injection in a Sandstone Reservoir

NASA Astrophysics Data System (ADS)

Wu, H.; Pollyea, R.

2017-12-01

Carbon capture and sequestration (CCS) is one component of a broad carbon management portfolio designed to mitigate adverse effects of anthropogenic CO2 emissions. During CCS, capillary trapping is an important mechanism for CO2 isolation in the disposal reservoir, and, as a result, the distribution of capillary force is an important factor affecting CO2 migration. Moreover, the movement of CO2 being injected to the reservoir is also affected by buoyancy, which results from the density difference between CO2 and brine. In order to understand interactions between capillary force and buoyancy, we implement a parametric modeling experiment of CO2 injections in a sandstone reservoir for combinations of the van Genuchten capillary pressure model that bound the range of capillary pressure-saturation curves measured in laboratory experiments. We simulate ten years supercritical CO2 (scCO2) injections within a 2-D radially symmetric sandstone reservoir for five combinations of the van Genuchten model parameters λ and entry pressure (P0). Results are analyzed on the basis of a modified dimensionless ratio, ω, which is similar to the Bond number and defines the relationship between buoyancy pressure and capillary pressure. We show how parametric variability affects the relationship between buoyancy and capillary force, and thus controls CO2 plume geometry. These results indicate that when ω >1, then buoyancy governs the system and CO2 plume geometry is governed by upward flow. In contrast, when ω <1, then buoyancy is smaller than capillary force and lateral flow governs CO2 plume geometry. As a result, we show that the ω ratio is an easily implemented screening tool for qualitative assessment of reservoir performance.

Numerical Modeling of Interstitial Fluid Flow Coupled with Blood Flow through a Remodeled Solid Tumor Microvascular Network

PubMed Central

Soltani, M.; Chen, P.

2013-01-01

Modeling of interstitial fluid flow involves processes such as fluid diffusion, convective transport in extracellular matrix, and extravasation from blood vessels. To date, majority of microvascular flow modeling has been done at different levels and scales mostly on simple tumor shapes with their capillaries. However, with our proposed numerical model, more complex and realistic tumor shapes and capillary networks can be studied. Both blood flow through a capillary network, which is induced by a solid tumor, and fluid flow in tumor’s surrounding tissue are formulated. First, governing equations of angiogenesis are implemented to specify the different domains for the network and interstitium. Then, governing equations for flow modeling are introduced for different domains. The conservation laws for mass and momentum (including continuity equation, Darcy’s law for tissue, and simplified Navier–Stokes equation for blood flow through capillaries) are used for simulating interstitial and intravascular flows and Starling’s law is used for closing this system of equations and coupling the intravascular and extravascular flows. This is the first study of flow modeling in solid tumors to naturalistically couple intravascular and extravascular flow through a network. This network is generated by sprouting angiogenesis and consisting of one parent vessel connected to the network while taking into account the non-continuous behavior of blood, adaptability of capillary diameter to hemodynamics and metabolic stimuli, non-Newtonian blood flow, and phase separation of blood flow in capillary bifurcation. The incorporation of the outlined components beyond the previous models provides a more realistic prediction of interstitial fluid flow pattern in solid tumors and surrounding tissues. Results predict higher interstitial pressure, almost two times, for realistic model compared to the simplified model. PMID:23840579

Capillary-Driven Flow in Liquid Filaments Connecting Orthogonal Channels

NASA Technical Reports Server (NTRS)

Allen, Jeffrey S.

2005-01-01

Capillary phenomena plays an important role in the management of product water in PEM fuel cells because of the length scales associated with the porous layers and the gas flow channels. The distribution of liquid water within the network of gas flow channels can be dramatically altered by capillary flow. We experimentally demonstrate the rapid movement of significant volumes of liquid via capillarity through thin liquid films which connect orthogonal channels. The microfluidic experiments discussed provide a good benchmark against which the proper modeling of capillarity by computational models may be tested. The effect of surface wettability, as expressed through the contact angle, on capillary flow will also be discussed.

Drag reducing polymers improve coronary flow reserve through modulation of capillary resistance.

PubMed

Pacella, John J; Kameneva, Marina V; Villanueva, Flordeliza S

2009-01-01

We have shown that drag-reducing polymers (DRP) reduce microvascular resistance and improve myocardial perfusion during coronary stenosis. We used myocardial contrast echocardiography (MCE) and mathematical modeling to define the DRP microvascular effects. A non-flow-limiting left anterior descending (LAD) stenosis was created in 8 dogs. Intramyocardial blood volume, RBC velocity and flow in the LAD and circumflex (CX) beds were obtained from MCE at baseline, and in hyperemia, stenosis, hyperemia + stenosis, and hyperemia + stenosis + DRP. Microvascular resistances were calculated from a lumped-parameter model. During stenosis + hyperemia, LAD bed microvascular resistance increased (p<0.015), and capillary volume (p<0.002) and red cell velocity (p<0.0004) decreased relative to baseline. With DRP, during stenosis and hyperemia, LAD bed microvascular resistance decreased (p<0.04); there was an increase in capillary volume (p<0.007), RBC velocity (p<0.006), and flow (p<0.05). Decreased model-computed capillary resistance accounted for the reduction in LAD bed resistance after DRP. We conclude that DRP improve flow reserve during coronary stenosis by modulating capillary resistance. Primary modification of the rheological properties of blood to affect capillary resistance is a novel approach for the treatment of acute coronary syndromes.

Analytical model for describing ion guiding through capillaries in insulating polymers

NASA Astrophysics Data System (ADS)

Liu, Shi-Dong; Zhao, Yong-Tao; Wang, Yu-Yu; N, Stolterfoht; Cheng, Rui; Zhou, Xian-Ming; Xu, Hu-Shan; Xiao, Guo-Qing

2015-08-01

An analytical description for guiding of ions through nanocapillaries is given on the basis of previous work. The current entering into the capillary is assumed to be divided into a current fraction transmitted through the capillary, a current fraction flowing away via the capillary conductivity and a current fraction remaining within the capillary, which is responsible for its charge-up. The discharging current is assumed to be governed by the Frenkel-Poole process. At higher conductivities the analytical model shows a blocking of the ion transmission, which is in agreement with recent simulations. Also, it is shown that ion blocking observed in experiments is well reproduced by the analytical formula. Furthermore, the asymptotic fraction of transmitted ions is determined. Apart from the key controlling parameter (charge-to-energy ratio), the ratio of the capillary conductivity to the incident current is included in the model. Differences resulting from the nonlinear and linear limits of the Frenkel-Poole discharge are pointed out. Project supported by the Major State Basic Research Development Program of China (Grant No. 2010CB832902) and the National Natural Science Foundation of China (Grant Nos. 11275241, 11275238, 11105192, and 11375034).

Waterspout as a result of the ocean skeletal structures

NASA Astrophysics Data System (ADS)

Rantsev-Kartinov, Valentin A.

2004-11-01

An analysis of databases of photographic images of oceanic surface, taken from various altitudes and for various types of rough ocean surface, reduced to a revealing the presence of oceanic skeletal structures (OSS) = http://www.arxiv.org/ftp/physics/papers/0401/0401139.pdf [1] Rantsev-Kartinov V.A., Preprint. The OSSs presumably differ from the formerly found skeletal structures (SS) (Phys. Lett. A 306 (2002) 175) only by the fact that OSS are filled in with the closely packed blocks of a smaller size, up to capillary sizes. It is suggested [1] the dust produced by the volcanic activity forms the SS of powerful clouds due to of atmospheric electricity. The fall-out of such SSs on the oceanic surface is a material source of OSS. It is suggested that an OSS block [1] in the form of vertically oriented floating cylinder may be a stimulator of waterspout (WS). The main body of the WS may be interpreted as a special type of atmospheric aerosol dusty plasma, and its column - as a long-lived filament, being formed in the process of electric breakdown between the cloud and oceanic surface. The charged water drops aerosol may behave similar to microdust and lift upward to the cloud by the electrostatic force. Suggested a capillary-electrostatic model of the WS permits to interpret many effects connected with the WS.

Dynamical clustering of red blood cells in capillary vessels.

PubMed

Boryczko, Krzysztof; Dzwinel, Witold; Yuen, David A

2003-02-01

We have modeled the dynamics of a 3-D system consisting of red blood cells (RBCs), plasma and capillary walls using a discrete-particle approach. The blood cells and capillary walls are composed of a mesh of particles interacting with harmonic forces between nearest neighbors. We employ classical mechanics to mimic the elastic properties of RBCs with a biconcave disk composed of a mesh of spring-like particles. The fluid particle method allows for modeling the plasma as a particle ensemble, where each particle represents a collective unit of fluid, which is defined by its mass, moment of inertia, translational and angular momenta. Realistic behavior of blood cells is modeled by considering RBCs and plasma flowing through capillaries of various shapes. Three types of vessels are employed: a pipe with a choking point, a curved vessel and bifurcating capillaries. There is a strong tendency to produce RBC clusters in capillaries. The choking points and other irregularities in geometry influence both the flow and RBC shapes, considerably increasing the clotting effect. We also discuss other clotting factors coming from the physical properties of blood, such as the viscosity of the plasma and the elasticity of the RBCs. Modeling has been carried out with adequate resolution by using 1 to 10 million particles. Discrete particle simulations open a new pathway for modeling the dynamics of complex, viscoelastic fluids at the microscale, where both liquid and solid phases are treated with discrete particles. Figure A snapshot from fluid particle simulation of RBCs flowing along a curved capillary. The red color corresponds to the highest velocity. We can observe aggregation of RBCs at places with the most stagnant plasma flow.

Nonequilibrium capillarity effects in multiphase flow through small volume fractured porous media

NASA Astrophysics Data System (ADS)

Tang, M.; Zhan, H.; Lu, S.

2017-12-01

Analyzing and understanding the capillary pressure curves in fractured porous media is a crucial subject in a number of industrial applications, such as crude oil recovery in the fractured reservoir, CO2 sequestration in fractured brine aquifers and shale gas development. Many studies have observed the significant nonequilibrium capillarity effects in multiphase flow through porous media and proposed that conventional equilibrium capillary pressure may not accurately describe transient two-phase flow behavior under dynamical conditions. To date, only several laboratory experiments and numerical models have been conducted into investigating the characteristic of nonequilibrium capillary pressure in unfractured porous media, a clear picture of the effects of fractures on the dynamic capillary pressure in fractured porous media remains elusive. In this study, four digital porous models were built based on CT image data from ZEISS Xradia 520 Versa CT scanning, a series of direct simulations of multiphase flow in fractured porous media were carried out based on lattice Boltzmann method and three-dimensional porous models. The results show that both the aperture and orientation of the fractures have significant effects on the nonequilibrium capillary pressure coefficients and multiphase flow behaviors. The nonequilibrium capillary pressure coefficients in fractured porous media are one to two orders of magnitude lower than unfractured porous media. This study presents a new direct simulation based methodology for the detailed analysis of nonequilibrium capillary pressure in fractured porous media.

An analytical model for enantioseparation process in capillary electrophoresis

NASA Astrophysics Data System (ADS)

Ranzuglia, G. A.; Manzi, S. J.; Gomez, M. R.; Belardinelli, R. E.; Pereyra, V. D.

2017-12-01

An analytical model to explain the mobilities of enantiomer binary mixture in capillary electrophoresis experiment is proposed. The model consists in a set of kinetic equations describing the evolution of the populations of molecules involved in the enantioseparation process in capillary electrophoresis (CE) is proposed. These equations take into account the asymmetric driven migration of enantiomer molecules, chiral selector and the temporary diastomeric complexes, which are the products of the reversible reaction between the enantiomers and the chiral selector. The solution of these equations gives the spatial and temporal distribution of each species in the capillary, reproducing a typical signal of the electropherogram. The mobility, μ, of each specie is obtained by the position of the maximum (main peak) of their respective distributions. Thereby, the apparent electrophoretic mobility difference, Δμ, as a function of chiral selector concentration, [ C ] , can be measured. The behaviour of Δμ versus [ C ] is compared with the phenomenological model introduced by Wren and Rowe in J. Chromatography 1992, 603, 235. To test the analytical model, a capillary electrophoresis experiment for the enantiomeric separation of the (±)-chlorpheniramine β-cyclodextrin (β-CD) system is used. These data, as well as, other obtained from literature are in closed agreement with those obtained by the model. All these results are also corroborate by kinetic Monte Carlo simulation.

Simulation of the Flow Through Porous Layers Composed of Converging-Diverging Capillary Fissures or Tubes

NASA Astrophysics Data System (ADS)

Walicka, A.

2018-02-01

In this paper, a porous medium is modelled by a network of converging-diverging capillaries which may be considered as fissures or tubes. This model makes it necessary to consider flows through capillary fissures or tubes. Therefore an analytical method for deriving the relationships between pressure drops, volumetric flow rates and velocities for the following fluids: Newtonian, polar, power-law, pseudoplastic (DeHaven and Sisko types) and Shulmanian, was developed. Next, considerations on the models of pore network for Newtonian and non-Newtonian fluids were presented. The models, similar to the schemes of central finite differences may provide a good basis for transforming the governing equations of a flow through the porous medium into a set of linear or quasi-linear algebraic equations. It was shown that the some coefficients in these algebraic equations depend on the kind of the capillary convergence.

Laser-driven acceleration of electrons in a partially ionized plasma channel.

PubMed

Rowlands-Rees, T P; Kamperidis, C; Kneip, S; Gonsalves, A J; Mangles, S P D; Gallacher, J G; Brunetti, E; Ibbotson, T; Murphy, C D; Foster, P S; Streeter, M J V; Budde, F; Norreys, P A; Jaroszynski, D A; Krushelnick, K; Najmudin, Z; Hooker, S M

2008-03-14

The generation of quasimonoenergetic electron beams, with energies up to 200 MeV, by a laser-plasma accelerator driven in a hydrogen-filled capillary discharge waveguide is investigated. Injection and acceleration of electrons is found to depend sensitively on the delay between the onset of the discharge current and the arrival of the laser pulse. A comparison of spectroscopic and interferometric measurements suggests that injection is assisted by laser ionization of atoms or ions within the channel.

Multifunctional Poro-Vascular Composites for UAV Performance Enhancement

DTIC Science & Technology

2012-07-31

structural “skin” materials with surface pores and internal vascular channels filled with an ionic- liquid whose height and shape at the pore exits is...2 V t e e q q          Intrinsic contact angle (zero voltage) Applied potential Interfacial tension (IFT) of ionic liquid Lippmann...Characterization flat plates  single (capillary) pore  PV pore arrays FTA 1000 Drop-Shape Characterization • Microscope lens : 0.5 to 12x magnification • Side

Realizing A Mid-Infrared Optically Pumped Molecular Gas Laser Inside Hollow-Core Photonic Crystal Fiber

DTIC Science & Technology

2012-01-01

54  Figure 5.2 External transmission of (a) BK7 glass , (b) CaF2, and (c) Germanium substrates of various thicknesses. The transmission values...a hollow, air-filled core surrounded by cladding structure which is comprised of a periodic array of smaller air holes. Unlike glass capillaries...region of the fiber’s core. The low overlap of the guided light with the HC-PCF’s glass structure together with a higher threshold for the onset of

A detailed study of CO 2-brine capillary trapping mechanisms as applied to geologic carbon storage. Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wildenschild, Dorthe

2017-04-06

The proposed research focuses on improved fundamental understanding of the efficiency of physical trapping mechanisms, and as such will provide the basis for subsequent upscaling efforts. The overarching hypothesis of the proposed research is that capillary pressure plays a significant role in capillary trapping of CO 2, especially during the water imbibition stage of the sequestration process. We posit that the relevant physics of the sequestration process is more complex than is currently captured in relative permeability models, which are often based on so-called trapping models to represent relative permeability hysteresis. Our 4 main questions, guiding the 4 main tasksmore » of the proposed research, are as follows: (1) What is the morphology of capillary trapped CO 2 at the pore scale as a function of temperature, pressure, brine concentration, interfacial tension, and pore-space morphology under injection and subsequent imbibition? (2) Is it possible to describe the capillary trapping process using formation-dependent, but otherwise unique continuum-scale functions in permeability-capillary pressure, interfacial area and saturation space, rather than hysteretic functions in permeability-saturation or capillary pressure-saturation space? (3) How do continuum-scale relationships between kr-Pc-S-Anw developed based on pore-scale observations compare with traditional models incorporating relative permeability hysteresis (such as Land’s and other models,) and with observations at the core (5-10cm) scale? (4) How can trapped CO 2 volume be optimized via engineered injection and sweep strategies, and as a function of formation type (incl. heterogeneity)?« less

On the Asymmetric Focusing of Low-Emittance Electron Bunches via Active Lensing by Using Capillary Discharges

NASA Astrophysics Data System (ADS)

Bulanov, Stepan; Bagdasarov, Gennadiy; Bobrova, Nadezhda; Boldarev, Alexey; Olkhovskaya, Olga; Sasorov, Pavel; Gasilov, Vladimir; Barber, Samuel; Gonsalves, Anthony; Schroeder, Carl; van Tilborg, Jeroen; Esarey, Eric; Leemans, Wim; Levato, Tadzio; Margarone, Daniele; Korn, Georg; Kando, Masaki; Bulanov, Sergei

2017-10-01

A novel method for asymmetric focusing of electron beams is proposed. The scheme is based on the active lensing technique, which takes advantage of the strong inhomogeneous magnetic field generated inside the capillary discharge plasma to focus the ultrarelativistic electrons. The plasma and magnetic field parameters inside a capillary discharge are described theoretically and modeled with dissipative MHD simulations to enable analysis of capillaries of oblong rectangle cross-sections implying that large aspect ratio rectangular capillaries can be used to form flat electron bunches. The effect of the capillary cross-section on the electron beam focusing properties were studied using the analytical methods and simulation- derived magnetic field map showing the range of the capillary discharge parameters required for producing the high quality flat electron beams.

An Earth-based Model of Microgravity Pulmonary Physiology

NASA Technical Reports Server (NTRS)

Hirschl, Ronald B.; Bull, Joseph L.; Grotberg, James B.

2004-01-01

There are currently only two practical methods of achieving microgravity for experimentation: parabolic flight in an aircraft or space flight, both of which have limitations. As a result, there are many important aspects of pulmonary physiology that have not been investigated in microgravity. We propose to develop an earth-based animal model of microgravity by using liquid ventilation, which will allow us to fill the lungs with perfluorocarbon, and submersing the animal in water such that the density of the lungs is the same as the surrounding environment. By so doing, we will eliminate the effects of gravity on respiration. We will first validate the model by comparing measures of pulmonary mechanics, to previous space flight and parabolic flight measurements. After validating the model, we will investigate the impact of microgravity on aspects of lung physiology that have not been previously measured. These will include pulmonary blood flow distribution, ventillation distribution, pulmonary capillary wedge pressure, ventilation-perfusion matching and pleural pressures and flows. We expect that this earth-based model of microgravity will enhance our knowledge and understanding of lung physiology in space which will increase in importance as space flights increase in time and distance.

Water infiltration in prewetted porous media: dynamic capillary pressure and Green-Ampt modeling

NASA Astrophysics Data System (ADS)

Hsu, S.; Hilpert, M.

2013-12-01

Recently, an experimental study has shown that the modified Green-Ampt (GA) model, which accounts for a velocity-dependent capillary pressure, can describe water infiltration in dry sand columns better than the classical GA model. Studies have also shown that the initial water content of prewetted porous media affects the dynamic capillary pressure during infiltration. In this study, we performed a series of downward water infiltration experiments in prewetted sand columns for four different initial water contents: 0%, 3.3%, 6.5%, and 13.8%. We also used three different ponding heights: 10 cm, 20 cm, and 40 cm. As expected, an increase in ponding height resulted in a monotonic increase in cumulative infiltration. However, we found anomalous behavior, in that the cumulative infiltration did not monotonically decrease as the initial water content increased. When modeling the experiments with the modified GA approach, we linked this anomalous behavior to the reduction factor in the model for dynamic capillary pressure that is a function of initial water content.

Competition between anisotropic viscous fingers

NASA Astrophysics Data System (ADS)

Pecelerowicz, M.; Budek, A.; Szymczak, P.

2014-09-01

We consider viscous fingers created by injection of low viscosity fluid into the network of capillaries initially filled with a more viscous fluid (motor oil). Due to the anisotropy of the system and its geometry, such a setup promotes the formation of long-and-thin fingers which then grow and compete for the available flow, interacting through the pressure field. The interaction between the fingers is analyzed using the branched growth formalism of Halsey and Leibig (Phys. Rev. A 46, 7723, 1992) using a number of simple, analytically tractable models. It is shown that as soon as the fingers are allowed to capture the flow from one another, the fixed point appears in the phase space, corresponding to the asymptotic state in which the growth of one of the fingers in hindered by the other. The properties of phase space flows in such systems are shown to be remarkably insensitive to the details of the dynamics.

Chiral analysis of UV nonabsorbing compounds by capillary electrophoresis using macrocyclic antibiotics: 1. Separation of aspartic and glutamic acid enantiomers.

PubMed

Bednar, P; Aturki, Z; Stransky, Z; Fanali, S

2001-07-01

Glycopeptide antibiotics, namely vancomycin or teicoplanin, were evaluated in capillary electrophoresis for the analysis of UV nonabsorbing compounds such as aspartic and glutamic acid enantiomers. Electrophoretic runs were performed in laboratory-made polyacrylamide-coated capillaries using the partial filling-counter current method in order to avoid the presence on the detector path of the absorbing chiral selector. The background electrolyte consisted of an aqueous or aqueous-organic buffer in the pH range of 4.5-6.5 of sorbic acid/histidine and the appropriate concentration of chiral selector. Several experimental parameters such as antibiotic concentration and type, buffer pH, organic modifier, type and concentration of absorbing co-ion (for the indirect UV detection) were studied in order to find the optimum conditions for the chiral resolution of the two underivatized amino acids in their enantiomers. Among the two investigated chiral selectors, vancomycin resulted to be the most useful chiral selector allowing relatively high chiral resolution of the studied compounds even at low concentration. The optimized method (10 mM sorbic acid/histidine, pH 5, and 10 mM of vancomycin) was used for the analysis of real samples such as teeth dentine and beer.

Liquid-metal plasma-facing component research on the National Spherical Torus Experiment

NASA Astrophysics Data System (ADS)

Jaworski, M. A.; Khodak, A.; Kaita, R.

2013-12-01

Liquid metal plasma-facing components (PFCs) have been proposed as a means of solving several problems facing the creation of economically viable fusion power reactors. Liquid metals face critical issues in three key areas: free-surface stability, material migration and demonstration of integrated scenarios. To date, few demonstrations exist of this approach in a diverted tokamak and we here provide an overview of such work on the National Spherical Torus Experiment (NSTX). The liquid lithium divertor (LLD) was installed and operated for the 2010 run campaign using evaporated coatings as the filling method. Despite a nominal liquid level exceeding the capillary structure and peak current densities into the PFCs exceeding 100 kA m-2, no macroscopic ejection events were observed. The stability can be understood from a Rayleigh-Taylor instability analysis. Capillary restraint and thermal-hydraulic considerations lead to a proposed liquid-metal PFCs scheme of actively-supplied, capillary-restrained systems. Even with state-of-the-art cooling techniques, design studies indicate that the surface temperature with divertor-relevant heat fluxes will still reach temperatures above 700 °C. At this point, one would expect significant vapor production from a liquid leading to a continuously vapor-shielded regime. Such high-temperature liquid lithium PFCs may be possible on the basis of momentum-balance arguments.

Modeling contribution of shallow groundwater to evapotranspiration and yield of maize in an arid area

NASA Astrophysics Data System (ADS)

Gao, Xiaoyu; Huo, Zailin; Qu, Zhongyi; Xu, Xu; Huang, Guanhua; Steenhuis, Tammo S.

2017-02-01

Capillary rise from shallow groundwater can decrease the need for irrigation water. However, simple techniques do not exist to quantify the contribution of capillary flux to crop water use. In this study we develop the Agricultural Water Productivity Model for Shallow Groundwater (AWPM-SG) for calculating capillary fluxes from shallow groundwater using readily available data. The model combines an analytical solution of upward flux from groundwater with the EPIC crop growth model. AWPM-SG was calibrated and validated with 2-year lysimetric experiment with maize. Predicted soil moisture, groundwater depth and leaf area index agreed with the observations. To investigate the response of model, various scenarios were run in which the irrigation amount and groundwater depth were varied. Simulations shows that at groundwater depth of 1 m capillary upward supplied 41% of the evapotranspiration. This reduced to 6% at groundwater depth of 2 m. The yield per unit water consumed (water productivity) was nearly constant for 2.3 kg/m3. The yield per unit water applied (irrigation water productivity) increased with decreasing irrigation water because capillary rise made up in part for the lack of irrigation water. Consequently, using AWPM-SG in irrigation scheduling will be beneficial to save more water in areas with shallow groundwater.

Investigation of the capillary flow through open surface microfluidic structures

NASA Astrophysics Data System (ADS)

Taher, Ahmed; Jones, Benjamin; Fiorini, Paolo; Lagae, Liesbet

2017-02-01

The passive nature of capillary microfluidics for pumping and actuation of fluids is attractive for many applications including point of care medical diagnostics. For such applications, there is often the need to spot dried chemical reagents in the bottom of microfluidic channels after device fabrication; it is often more practical to have open surface devices (i.e., without a cover or lid). However, the dynamics of capillary driven flow in open surface devices have not been well studied for many geometries of interest. In this paper, we investigate capillary flow in an open surface microchannel with a backward facing step. An analytical model is developed to calculate the capillary pressure as the liquid-vapor interface traverses a backward facing step in an open microchannel. The developed model is validated against results from Surface Evolver liquid-vapor surface simulations and ANSYS Fluent two-phase flow simulations using the volume of fluid approach. Three different aspect ratios (inlet channel height by channel width) were studied. The analytical model shows good agreement with the simulation results from both modeling methods for all geometries. The analytical model is used to derive an expression for the critical aspect ratio (the minimum channel aspect ratio for flow to proceed across the backward facing step) as a function of contact angle.

Modeling contribution of shallow groundwater to evapotranspiration and yield of maize in an arid area

PubMed Central

Gao, Xiaoyu; Huo, Zailin; Qu, Zhongyi; Xu, Xu; Huang, Guanhua; Steenhuis, Tammo S.

2017-01-01

Capillary rise from shallow groundwater can decrease the need for irrigation water. However, simple techniques do not exist to quantify the contribution of capillary flux to crop water use. In this study we develop the Agricultural Water Productivity Model for Shallow Groundwater (AWPM-SG) for calculating capillary fluxes from shallow groundwater using readily available data. The model combines an analytical solution of upward flux from groundwater with the EPIC crop growth model. AWPM-SG was calibrated and validated with 2-year lysimetric experiment with maize. Predicted soil moisture, groundwater depth and leaf area index agreed with the observations. To investigate the response of model, various scenarios were run in which the irrigation amount and groundwater depth were varied. Simulations shows that at groundwater depth of 1 m capillary upward supplied 41% of the evapotranspiration. This reduced to 6% at groundwater depth of 2 m. The yield per unit water consumed (water productivity) was nearly constant for 2.3 kg/m3. The yield per unit water applied (irrigation water productivity) increased with decreasing irrigation water because capillary rise made up in part for the lack of irrigation water. Consequently, using AWPM-SG in irrigation scheduling will be beneficial to save more water in areas with shallow groundwater. PMID:28220874

Modeling contribution of shallow groundwater to evapotranspiration and yield of maize in an arid area.

PubMed

Gao, Xiaoyu; Huo, Zailin; Qu, Zhongyi; Xu, Xu; Huang, Guanhua; Steenhuis, Tammo S

2017-02-21

Capillary rise from shallow groundwater can decrease the need for irrigation water. However, simple techniques do not exist to quantify the contribution of capillary flux to crop water use. In this study we develop the Agricultural Water Productivity Model for Shallow Groundwater (AWPM-SG) for calculating capillary fluxes from shallow groundwater using readily available data. The model combines an analytical solution of upward flux from groundwater with the EPIC crop growth model. AWPM-SG was calibrated and validated with 2-year lysimetric experiment with maize. Predicted soil moisture, groundwater depth and leaf area index agreed with the observations. To investigate the response of model, various scenarios were run in which the irrigation amount and groundwater depth were varied. Simulations shows that at groundwater depth of 1 m capillary upward supplied 41% of the evapotranspiration. This reduced to 6% at groundwater depth of 2 m. The yield per unit water consumed (water productivity) was nearly constant for 2.3 kg/m 3 . The yield per unit water applied (irrigation water productivity) increased with decreasing irrigation water because capillary rise made up in part for the lack of irrigation water. Consequently, using AWPM-SG in irrigation scheduling will be beneficial to save more water in areas with shallow groundwater.

Thermodynamic stability of nanosized multicomponent bubbles/droplets: the square gradient theory and the capillary approach.

PubMed

Wilhelmsen, Øivind; Bedeaux, Dick; Kjelstrup, Signe; Reguera, David

2014-01-14

Formation of nanosized droplets/bubbles from a metastable bulk phase is connected to many unresolved scientific questions. We analyze the properties and stability of multicomponent droplets and bubbles in the canonical ensemble, and compare with single-component systems. The bubbles/droplets are described on the mesoscopic level by square gradient theory. Furthermore, we compare the results to a capillary model which gives a macroscopic description. Remarkably, the solutions of the square gradient model, representing bubbles and droplets, are accurately reproduced by the capillary model except in the vicinity of the spinodals. The solutions of the square gradient model form closed loops, which shows the inherent symmetry and connected nature of bubbles and droplets. A thermodynamic stability analysis is carried out, where the second variation of the square gradient description is compared to the eigenvalues of the Hessian matrix in the capillary description. The analysis shows that it is impossible to stabilize arbitrarily small bubbles or droplets in closed systems and gives insight into metastable regions close to the minimum bubble/droplet radii. Despite the large difference in complexity, the square gradient and the capillary model predict the same finite threshold sizes and very similar stability limits for bubbles and droplets, both for single-component and two-component systems.

Thermodynamic stability of nanosized multicomponent bubbles/droplets: The square gradient theory and the capillary approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilhelmsen, Øivind, E-mail: oivind.wilhelmsen@ntnu.no; Bedeaux, Dick; Kjelstrup, Signe

Formation of nanosized droplets/bubbles from a metastable bulk phase is connected to many unresolved scientific questions. We analyze the properties and stability of multicomponent droplets and bubbles in the canonical ensemble, and compare with single-component systems. The bubbles/droplets are described on the mesoscopic level by square gradient theory. Furthermore, we compare the results to a capillary model which gives a macroscopic description. Remarkably, the solutions of the square gradient model, representing bubbles and droplets, are accurately reproduced by the capillary model except in the vicinity of the spinodals. The solutions of the square gradient model form closed loops, which showsmore » the inherent symmetry and connected nature of bubbles and droplets. A thermodynamic stability analysis is carried out, where the second variation of the square gradient description is compared to the eigenvalues of the Hessian matrix in the capillary description. The analysis shows that it is impossible to stabilize arbitrarily small bubbles or droplets in closed systems and gives insight into metastable regions close to the minimum bubble/droplet radii. Despite the large difference in complexity, the square gradient and the capillary model predict the same finite threshold sizes and very similar stability limits for bubbles and droplets, both for single-component and two-component systems.« less

Study of the water transportation characteristics of marsh saline soil in the Yellow River Delta.

PubMed

He, Fuhong; Pan, Yinghua; Tan, Lili; Zhang, Zhenhua; Li, Peng; Liu, Jia; Ji, Shuxin; Qin, Zhaohua; Shao, Hongbo; Song, Xueyan

2017-01-01

One-dimensional soil column water infiltration and capillary adsorption water tests were conducted in the laboratory to study the water transportation characteristics of marsh saline soil in the Yellow River Delta, providing a theoretical basis for the improvement, utilization and conservation of marsh saline soil. The results indicated the following: (1) For soils with different vegetation covers, the cumulative infiltration capacity increased with the depth of the soil layers. The initial infiltration rate of soils covered by Suaeda and Tamarix chinensis increased with depth of the soil layers, but that of bare soil decreased with soil depth. (2) The initial rate of capillary rise of soils with different vegetation covers showed an increasing trend from the surface toward the deeper layers, but this pattern with respect to soil depth was relatively weak. (3) The initial rates of capillary rise were lower than the initial infiltration rates, but infiltration rate decreased more rapidly than capillary water adsorption rate. (4) The two-parameter Kostiakov model can very well-simulate the changes in the infiltration and capillary rise rates of wetland saline soil. The model simulated the capillary rise rate better than it simulated the infiltration rate. (5) There were strong linear relationships between accumulative infiltration capacity, wetting front, accumulative capillary adsorbed water volume and capillary height. Copyright © 2016 Elsevier B.V. All rights reserved.

Modelling the hydraulic conductivity of porous media using physical-statistical model

NASA Astrophysics Data System (ADS)

Usowicz, B.; Usowicz, L. B.; Lipiec, J.

2009-04-01

Soils and other porous media can be represented by a pattern (net) of more or less cylindrically interconnected channels. The capillary radius, r can represent an elementary capillary formed in between soil particles in one case, and in another case it can represent a mean hydrodynamic radius. When we view a porous medium as a net of interconnected capillaries, we can apply a statistical approach for the description of the liquid or gas flow. A soil phase is included in the porous medium and its configuration is decisive for pore distribution in this medium and hence, it conditions the course of the water retention curve of this medium. In this work method of estimating hydraulic conductivity of porous media based on physical-statistical model proposed by B. Usowicz is presented. The physical-statistical model considers the pore space as the capillary net. The net of capillary connections is represented by parallel and serial connections of hydraulic resistors in the layer and between the layers, respectively. The polynomial distribution was used in this model to determine probability of the occurrence of a given capillary configuration. The model was calibrated using measured water retention curve and two values of hydraulic conductivity saturated and unsaturated and model parameters were determined. The model was used for predicting hydraulic conductivity as a function of soil water content K(theta). The model was validated by comparing the measured and predicted K data for various soils and other porous media (e.g. sandstone). A good agreement between measured and predicted data was reasonable as indicated by values R2 (>0.9). It was also confirmed that the random variables used for the calculations and model parameters were chosen and selected correctly. The study was funded in part by the Polish Ministry of Science and Higher Education by Grant No. N305 046 31/1707).

Modeling steady state SO2-dependent changes in capillary ATP concentration using novel O2 micro-delivery methods

PubMed Central

Ghonaim, Nour W.; Fraser, Graham M.; Ellis, Christopher G.; Yang, Jun; Goldman, Daniel

2013-01-01

Adenosine triphosphate (ATP) is known to be released from the erythrocyte in an oxygen (O2) dependent manner. Since ATP is a potent vasodilator, it is proposed to be a key regulator in the pathway that mediates micro-vascular response to varying tissue O2 demand. We propose that ATP signaling mainly originates in the capillaries due to the relatively long erythrocyte transit times in the capillary and the short ATP diffusion distance to the electrically coupled endothelium. We have developed a computational model to investigate the effect of delivering or removing O2 to limited areas at the surface of a tissue with an idealized parallel capillary array on total ATP concentration. Simulations were conducted when exposing full surface to perturbations in tissue O2 tension (PO2) or locally using a circular micro-outlet (~100 μm in diameter), a square micro-slit (200 × 200 μm), or a rectangular micro-slit (1000 μm wide × 200 μm long). Results indicated the rectangular micro-slit has the optimal dimensions for altering hemoglobin saturations (SO2) in sufficient number capillaries to generate effective changes in total [ATP]. This suggests a threshold for the minimum number of capillaries that need to be stimulated in vivo by imposed tissue hypoxia to induce a conducted micro-vascular response. SO2 and corresponding [ATP] changes were also modeled in a terminal arteriole (9 μm in diameter) that replaces 4 surface capillaries in the idealized network geometry. Based on the results, the contribution of terminal arterioles to the net change in [ATP] in the micro-vascular network is minimal although they would participate as O2 sources thus influencing the O2 distribution. The modeling data presented here provide important insights into designing a novel micro-delivery device for studying micro-vascular O2 regulation in the capillaries in vivo. PMID:24069001

Subtle exchange model of flow depended on the blood cell shape to enhance the micro-circulation in capillary

NASA Astrophysics Data System (ADS)

Chan, Iatneng

2012-02-01

In general the exchange of gases or other material in capillary system is conceptualized by the diffusion effect. But in this model, we investigate a micro-flow pattern by simulation and computation on a micro-exchange model in which the blood cell is a considered factor, especially on its shape. It shows that the cell benefits the circulation while it is moving in the capillary. In the study, the flow detail near the cell surface is mathematically analyzed, such that the Navier-Stokes equations are applied and the viscous factor is also briefly considered. For having a driven force to the motion of micro-circulation, a breathing mode is suggested to approximately compute on the flow rate in the blood capillary during the transfer of cell. The rate is also used to estimate the enhancement to the circulation in additional to the outcome of diffusion. Moreover in the research, the shape change of capillary wall under pressure influence is another element in the beginning calculation for the effect in the assistance to cell motion.

In Situ Determination of Illegal Drugs in Oral Fluid by Portable Capillary Electrophoresis with Deep UV Excited Fluorescence Detection.

PubMed

Saar-Reismaa, Piret; Erme, Enn; Vaher, Merike; Kulp, Maria; Kaljurand, Mihkel; Mazina-Šinkar, Jekaterina

2018-05-15

The present study demonstrates the potential of a portable capillary electrophoresis (CE) instrument, coupled to deep UV fluorescence detector (FD) with a 230-255 nm excitation wavelength range, for the determination of the abuse of illegal drugs in oral fluids in situ. CE was introduced in this study due its exceptional power of separation and resolution, short analysis time, and ability for miniaturization for on-site assessment of different substances. The deep UV fluorescence detector was equipped with five interchangeable emission filters, in the emission wavelength range from 278 to 600 nm, and was successfully employed for determination of natively fluorescing illegal drugs, such as cocaine, cocaethylene, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxeamphetamine (MDA), 3,4-methylenedioxy- N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxy- N-methylamphetamine (PMMA), amphetamine (AMP), methamphetamine (METH), tetrahydrocannabinol (THC) and cannabidiol (CBD). The developed FD showed impressive sensitivity. The instrumental detection limit was 0.5 μg/L for MDMA. It also showed broad linearity, up to 50 mg/L for MDMA. The noise CV% was 1.1% for an empty capillary and 0.6% for a capillary filled with acetonitrile. The portable CE-FD with developed electrophoretic methodologies was successfully utilized for the determination of illegal abuse of drugs during "Weekend" 2016 and 2017 Music Festivals (Estonia). Moreover, CE-FD can be employed for detection of other natively fluorescing compounds in the proposed range (e.g., for different phenolic compounds, BTEX, naphthalene derivatives, and others), significantly widening the applicability of developed CE-FD instrument.

A visualization study on two-phase gravity drainage in porous media by using magnetic resonance imaging.

PubMed

Teng, Ying; Liu, Yu; Jiang, Lanlan; Song, Yongchen; Zhao, Jiafei; Zhang, Yi; Wang, Dayong

2016-09-01

Gravity drainage characteristics are important to improve our understanding of gas-liquid or liquid-liquid two-phase flow in porous media. Stable or unstable displacement fronts that controlled by the capillary force, viscous force, gravitational force, etc., are relevant features of immiscible two-phase flow. In this paper, three dimensionless parameters, namely, the gravity number, the capillary number and the Bond number, were used to describe the effect of the above mentioned forces on two-phase drainage features, including the displacement front and final displacing-phase saturation. A series of experiments on the downward displacement of a viscous fluid by a less viscous fluid in a vertical vessel that is filled with quartz beads are performed by using magnetic resonance imaging (MRI). The experimental results indicate that the wetting properties at both high and low capillary numbers exert remarkable control on the fluid displacement. When the contact angle is lower than 90°, i.e., the displaced phase is the wetting phase, the average velocity Vf of the interface of the two phases (displacement front velocity) is observably lower than when the displaced phase is the non-wetting phase (contact angle higher than 90°). The results show that a fingering phenomenon occurs when the gravity number G is less than the critical gravity number G'=Δμ/μg. Moreover, the higher Bond number results in higher final displacing-phase saturation, whereas the capillary number has an opposite effect. Copyright © 2016 Elsevier Inc. All rights reserved.

Construction of stable capillary networks using a microfluidic device.

PubMed

Sudo, Ryo

2015-01-01

Construction of stable capillary networks is required to provide sufficient oxygen and nutrients to the deep region of thick tissues, which is important in the context of 3D tissue engineering. Although conventional in vitro culture models have been used to investigate the mechanism of capillary formation, recent advances in microfluidics technologies allowed us to control biophysical and biochemical culture environments more precisely, which led to the construction of functional and stable capillary networks. In this study, endothelial cells and mesenchymal stem cells were co-cultured in microfluidic devices to construct stable capillary networks, which resulted in the construction of luminal structures covered by pericytes. Interactions between endothelial cells and mesenchymal stem cells are also discussed in the context of capillary formation.

Water movement in glass bead porous media: 1. Experiments of capillary rise and hysteresis

NASA Astrophysics Data System (ADS)

Lu, T. X.; Biggar, J. W.; Nielsen, D. R.

1994-12-01

Experimental observations of capillary rise and hysteresis of water or ethanol in glass beads are presented to improve our understanding of those physical processes in porous media. The results provide evidence that capillary rise into porous media cannot be fully explained by a model of cylinders. They further demonstrate that the "Ink bottle" model does not provide an adequate explanation of hysteresis. Glass beads serving as a model for ideal soil are enclosed in a rectangular glass chamber model. A TV camera associated with a microscope was used to record the processes of capillary rise and drainage. It is clearly shown during capillary rise that the fluid exhibits a "jump" behavior at the neck of the pores in an initially dry profile or at the bottom of the water film in an initially wet profile. Under an initially dry condition, the jump initiates at the particle with smallest diameter. The jump process continues to higher elevations until at equilibrium the surface tensile force is balanced by the hydrostatic force. The wetting front at that time is readily observed as flat and saturated. Under an initially wet condition, capillary rise occurs as a water film thickening process associated with the jump process. Trapped air behind the wetting front renders the wetting front irregular and unsaturated. The capillary rise into an initially wet porous medium can be higher than that into an initially dry profile. During the drying process, large surface areas associated with the gas-liquid interface develop, allowing the porous medium to retain more water than during the wetting process at the same pressure. That mechanism explains better the hysteresis phenomenon in porous media in contrast to other mechanisms that now prevail.

Self-interference fluorescence microscopy with three-phase detection for depth-resolved confocal epi-fluorescence imaging.

PubMed

Braaf, Boy; de Boer, Johannes F

2017-03-20

Three-dimensional confocal fluorescence imaging of in vivo tissues is challenging due to sample motion and limited imaging speeds. In this paper a novel method is therefore presented for scanning confocal epi-fluorescence microscopy with instantaneous depth-sensing based on self-interference fluorescence microscopy (SIFM). A tabletop epi-fluorescence SIFM setup was constructed with an annular phase plate in the emission path to create a spectral self-interference signal that is phase-dependent on the axial position of a fluorescent sample. A Mach-Zehnder interferometer based on a 3 × 3 fiber-coupler was developed for a sensitive phase analysis of the SIFM signal with three photon-counter detectors instead of a spectrometer. The Mach-Zehnder interferometer created three intensity signals that alternately oscillated as a function of the SIFM spectral phase and therefore encoded directly for the axial sample position. Controlled axial translation of fluorescent microsphere layers showed a linear dependence of the SIFM spectral phase with sample depth over axial image ranges of 500 µm and 80 µm (3.9 × Rayleigh range) for 4 × and 10 × microscope objectives respectively. In addition, SIFM was in good agreement with optical coherence tomography depth measurements on a sample with indocyanine green dye filled capillaries placed at multiple depths. High-resolution SIFM imaging applications are demonstrated for fluorescence angiography on a dye-filled capillary blood vessel phantom and for autofluorescence imaging on an ex vivo fly eye.

Relationship of echocardiographic indices to pulmonary capillary wedge pressures in healthy volunteers

NASA Technical Reports Server (NTRS)

Firstenberg, M. S.; Levine, B. D.; Garcia, M. J.; Greenberg, N. L.; Cardon, L.; Morehead, A. J.; Zuckerman, J.; Thomas, J. D.

2000-01-01

OBJECTIVES: We sought to determine the relationship between different echocardiographic indices and pulmonary capillary wedge pressures (PCWP) in normal volunteers. BACKGROUND: Indices based on tissue Doppler (TDE) and color M-mode (CMM) echocardiography have been proposed to reflect left (LV) ventricular filling pressures. These include the ratio of early diastolic transmitral velocity (E) to early myocardial velocity measured by TDE (E') and the ratio of E to the wave propagation velocity (Vp) measured from CMM images. These indices, however, have not been validated in normal individuals. METHODS: We studied seven volunteers during two phases of preload altering maneuvers, baseline, with two stages of lower body negative pressure, and repeat baseline with two stages of volume loading. The PCWP obtained from right heart catheterization was compared with diastolic indices using pulsed Doppler, TDE and CMM echocardiography. RESULTS: The PCWP ranged from 2.2 to 23.5 mm Hg. During preload alterations, significant changes in E and septal E' (both p < 0.05) but not lateral E' or Vp were observed. Furthermore, E, septal E' and E/Vp correlated with PCWP (all r > 0.80) but not combined E and TDE indices (both r < 0.15). Within individuals, a similar linear relationship was observed among E/Vp, E and septal E' (average r > 0.80). CONCLUSIONS: In subjects without heart disease, E, septal E' and E/Vp correlate with PCWP. Because the influence of ventricular relaxation is minimized, the ratio E/Vp may be the best overall index of LV filling pressures.

Interstitial distribution of charged macromolecules in the dog lung: a kinetic model.

PubMed

Parker, J C; Miniati, M; Pitt, R; Taylor, A E

1987-01-01

A mathematic model was constructed to investigate conflicting physiologic data concerning the charge effect of continuous capillaries to macromolecules in the lung. We simulated the equilibration kinetics of lactate dehydrogenase (MR 4.2 nM) isozymes LDH 1 (pI = 5.0) and LDH 5 (pI = 7.9) between plasma and lymph using previously measured permeability coefficients, lung tissue distribution volumes (VA) and plasma concentrations (CP) in lung tissue. Our hypothesis is that the fixed anionic charges in interstitium, basement membrane, and cell surfaces determine equilibration rather than charged membrane effects at the capillary barrier, so the same capillary permeability coefficients were used for both isozymes. Capillary filtration rates and protein fluxes were calculated using conventional flux equations. Initial conditions at baseline and increased left atrial pressures (PLA) were those measured in animal studies. Simulated equilibration of isozymes over 30 h in the model at baseline capillary pressures accurately predicted the observed differences in lymph/plasma concentration ratios (CL/CP) between isotopes at 4 h and equilibration of these ratios at 24 h. Quantitative prediction of isozyme CL/CP ratios was also obtained at increased PLA. However, an additional cation selective compartment representing the surface glycocalyx was required to accurately simulate the initial higher transcapillary clearances of cationic LDH 5. Thus experimental data supporting the negative barrier, positive barrier, and no charge barrier hypotheses were accurately reproduced by the model using only the observed differences in interstitial partitioning of isozymes without differences in capillary selectivity.

Capillary device refilling. [liquid rocket propellant tank tests

NASA Technical Reports Server (NTRS)

Blatt, M. H.; Merino, F.; Symons, E. P.

1980-01-01

An analytical and experimental study was conducted dealing with refilling start baskets (capillary devices) with settled fluid. A computer program was written to include dynamic pressure, screen wicking, multiple-screen barriers, standpipe screens, variable vehicle mass for computing vehicle acceleration, and calculation of tank outflow rate and vapor pullthrough height. An experimental apparatus was fabricated and tested to provide data for correlation with the analytical model; the test program was conducted in normal gravity using a scale-model capillary device and ethanol as the test fluid. The test data correlated with the analytical model; the model is a versatile and apparently accurate tool for predicting start basket refilling under actual mission conditions.

Capillary transit time heterogeneity and flow-metabolism coupling after traumatic brain injury

PubMed Central

Østergaard, Leif; Engedal, Thorbjørn S; Aamand, Rasmus; Mikkelsen, Ronni; Iversen, Nina K; Anzabi, Maryam; Næss-Schmidt, Erhard T; Drasbek, Kim R; Bay, Vibeke; Blicher, Jakob U; Tietze, Anna; Mikkelsen, Irene K; Hansen, Brian; Jespersen, Sune N; Juul, Niels; Sørensen, Jens CH; Rasmussen, Mads

2014-01-01

Most patients who die after traumatic brain injury (TBI) show evidence of ischemic brain damage. Nevertheless, it has proven difficult to demonstrate cerebral ischemia in TBI patients. After TBI, both global and localized changes in cerebral blood flow (CBF) are observed, depending on the extent of diffuse brain swelling and the size and location of contusions and hematoma. These changes vary considerably over time, with most TBI patients showing reduced CBF during the first 12 hours after injury, then hyperperfusion, and in some patients vasospasms before CBF eventually normalizes. This apparent neurovascular uncoupling has been ascribed to mitochondrial dysfunction, hindered oxygen diffusion into tissue, or microthrombosis. Capillary compression by astrocytic endfeet swelling is observed in biopsies acquired from TBI patients. In animal models, elevated intracranial pressure compresses capillaries, causing redistribution of capillary flows into patterns argued to cause functional shunting of oxygenated blood through the capillary bed. We used a biophysical model of oxygen transport in tissue to examine how capillary flow disturbances may contribute to the profound changes in CBF after TBI. The analysis suggests that elevated capillary transit time heterogeneity can cause critical reductions in oxygen availability in the absence of ‘classic' ischemia. We discuss diagnostic and therapeutic consequences of these predictions. PMID:25052556

On production and asymmetric focusing of flat electron beams using rectangular capillary discharge plasmas

DOE PAGES

Bagdasarov, G. A.; Bobrova, N. A.; Boldarev, A. S.; ...

2017-12-27

A method for the asymmetric focusing of electron bunches, based on the active plasma lensing technique is proposed. Our method takes advantage of the strong inhomogeneous magnetic field generated inside the capillary discharge plasma to focus the ultrarelativistic electrons. The plasma and magnetic field parameters inside the capillary discharge are described theoretically and modeled with dissipative magnetohydrodynamic computer simulations enabling analysis of the capillaries of rectangle cross-sections. We could use large aspect ratio rectangular capillaries to transport electron beams with high emittance asymmetries, as well as assist in forming spatially flat electron bunches for final focusing before the interaction point.

On production and asymmetric focusing of flat electron beams using rectangular capillary discharge plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bagdasarov, G. A.; Bobrova, N. A.; Boldarev, A. S.

A method for the asymmetric focusing of electron bunches, based on the active plasma lensing technique is proposed. Our method takes advantage of the strong inhomogeneous magnetic field generated inside the capillary discharge plasma to focus the ultrarelativistic electrons. The plasma and magnetic field parameters inside the capillary discharge are described theoretically and modeled with dissipative magnetohydrodynamic computer simulations enabling analysis of the capillaries of rectangle cross-sections. We could use large aspect ratio rectangular capillaries to transport electron beams with high emittance asymmetries, as well as assist in forming spatially flat electron bunches for final focusing before the interaction point.

On production and asymmetric focusing of flat electron beams using rectangular capillary discharge plasmas

NASA Astrophysics Data System (ADS)

Bagdasarov, G. A.; Bobrova, N. A.; Boldarev, A. S.; Olkhovskaya, O. G.; Sasorov, P. V.; Gasilov, V. A.; Barber, S. K.; Bulanov, S. S.; Gonsalves, A. J.; Schroeder, C. B.; van Tilborg, J.; Esarey, E.; Leemans, W. P.; Levato, T.; Margarone, D.; Korn, G.; Kando, M.; Bulanov, S. V.

2017-12-01

A method for the asymmetric focusing of electron bunches, based on the active plasma lensing technique, is proposed. This method takes advantage of the strong inhomogeneous magnetic field generated inside the capillary discharge plasma to focus on the ultrarelativistic electrons. The plasma and magnetic field parameters inside the capillary discharge are described theoretically and modeled with dissipative magnetohydrodynamic computer simulations enabling analysis of the capillaries of rectangle cross-sections. Large aspect ratio rectangular capillaries might be used to transport electron beams with high emittance asymmetries, as well as assist in forming spatially flat electron bunches for final focusing before the interaction point.

Mechanism of oil bank formation, coalescence in porous media and emulsion and foam stability. Quarterly research progress report, July 1, 1984-September 30, 1984

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wasan, D.T.

The relative permeability model for two phase flow in porous media (Wasan 1983; Ramakrishnan and Wasan 1984) provides the necessary fractional flow curves at a given capillary number. These curves can be utilized in modeling both enhanced secondary and tertiary recovery processes. Important parameters in the fractional flow curves of our relative permeability model are the residual wetting and nonwetting phase saturations in a low capillary number flooding process. To understand, what constitutes the residual saturations, this quarter we have studied the displacement of one incompressible fluid by another in a porous medium using the network representation. The Bernoulli percolationmore » model for an infinite lattice graph is utilized in the interpretation of the capillary behavior of the medium, which ultimately determines residual saturations. The calculated capillary pressure-saturation relationship using Bethe lattice results agrees qualitatively with experimental data. 4 references, 2 figures.« less

Easy-to-use microfluidic chip for long-term 3D-cell cultures

NASA Astrophysics Data System (ADS)

Bunge, Frank; van den Driesche, Sander; Vellekoop, Michael J.

2017-05-01

We present a microfluidic chip for an easy setup of a 3D-culture of mammalian cells. The chip contains feeding structures and gas supply for long-term cultivation of mammalian cells. The device is fabricated out of hard materials like silicon and glass that are all highly biocompatible. The chip uses the concept of surficial phaseguides that allows the partial filling of a microfluidic chip with liquids based on hydrophobic and hydrophilic surfaces. Here, a suspension of mammalian cells and melted agarose is filled into the chip and is pulled by the capillary pressure on the hydrophilic areas but not on the hydrophobic phaseguides. Consequently, only a part of the chip is filled with the agarose which gels by cooling a form the 3D-cell culture. The unfilled areas are used as supply structures for nutrition and gases. So the supply is based on diffusion and the supply of nutrition and gases is controlled independently. We cultured HaCaT-cells over 24 hours in our device and achieve a good viability.

Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions.

PubMed

Masum, Md Abdul; Ichii, Osamu; Elewa, Yaser Hosny Ali; Nakamura, Teppei; Kon, Yasuhiro

2017-09-04

The renal vasculature plays important roles in both homeostasis and pathology. In this study, we examined pathological changes in the renal microvascular in mouse models of kidney diseases. Glomerular lesions (GLs) in autoimmune disease-prone male BXSB/MpJ-Yaa (Yaa) mice and tubulointerstitial lesions (TILs) in male C57BL/6 mice subjected to unilateral ureteral obstruction (UUO) for 7 days were studied. Collected kidneys were examined using histopathological techniques. A nonparametric Mann-Whitney U test (P < 0.05) was performed to compare healthy controls and the experimental mice. The Kruskal-Wallis test was used to compare three or more groups, and multiple comparisons were performed using Scheffe's method when significant differences were observed (P < 0.05). Yaa mice developed severe autoimmune glomerulonephritis, and the number of CD34 + glomerular capillaries decreased significantly in GLs compared to that in control mice. However, UUO-treated mice showed severe TILs only, and CD34 + tubulointerstitial capillaries were decreased significantly in TILs with the progression of tubulointerstitial fibrosis compared to those in untreated control kidneys. Infiltrations of B-cells, T-cells, and macrophages increased significantly in the respective lesions of both disease models (P < 0.05). In observations of vascular corrosion casts by scanning electron microscopy and of microfil rubber-perfused thick kidney sections by fluorescence microscopy, segmental absences of capillaries were observed in the GLs and TILs of Yaa and UUO-treated mice, respectively. Further, transmission electron microscopy revealed capillary endothelial injury in the respective lesions of both models. The numbers of CD34 + glomerular and tubulointerstitial capillaries were negatively correlated with all examined parameters in GLs (P < 0.05) and TILs (P < 0.01), respectively. From the analysis of mouse models, we identified inverse pathological correlations between the number of local capillaries in GLs and TILs and the severity of kidney diseases.

Surface Deformation by Thermo-capillary Convection -Sounding Rocket COMPERE Experiment SOURCE

NASA Astrophysics Data System (ADS)

Fuhrmann, Eckart; Dreyer, Michael E.

The sounding rocket COMPERE experiment SOURCE was successfully flown on MASER 11, launched in Kiruna (ESRANGE), May 15th, 2008. SOURCE has been intended to partly ful-fill the scientific objectives of the European Space Agency (ESA) Microgravity Applications Program (MAP) project AO-2004-111 (Convective boiling and condensation). Three parties of principle investigators have been involved to design the experiment set-up: ZARM for thermo-capillary flows, IMFT (Toulouse, France) for boiling studies, EADS Astrium (Bremen, Ger-many) for depressurization. The scientific aims are to study the effect of wall heat flux on the contact line of the free liquid surface and to obtain a correlation for a convective heat transfer coefficient. The experiment has been conducted along a predefined time line. A preheating sequence at ground was the first operation to achieve a well defined temperature evolution within the test cell and its environment inside the rocket. Nearly one minute after launch, the pressurized test cell was filled with the test liquid HFE-7000 until a certain fill level was reached. Then the free surface could be observed for 120 s without distortion. Afterwards, the first depressurization was started to induce subcooled boiling, the second one to start saturated boiling. The data from the flight consists of video images and temperature measurements in the liquid, the solid, and the gaseous phase. Data analysis provides the surface shape versus time and the corresponding apparent contact angle. Computational analysis provides information for the determination of the heat transfer coefficient in a compensated gravity environment where a flow is caused by the temperature difference between the hot wall and the cold liquid. Correlations for the effective contact angle and the heat transfer coefficient shall be delivered as a function of the relevant dimensionsless parameters. The data will be used for benchmarking of commercial CFD codes and the tank design (using these codes) of future cryogenic upper stages.

An air-pressure-free elastomeric valve for integrated nucleic acid analysis by capillary electrophoresis

NASA Astrophysics Data System (ADS)

Jung, Wooseok; Barrett, Matthew; Brooks, Carla; Rivera, Andrew; Birdsell, Dawn N.; Wagner, David M.; Zenhausern, Frederic

2015-12-01

We present a new elastomeric valve for integrated nucleic acid analysis by capillary electrophoresis. The valve functions include metering to capture a designated volume of biological sample into a polymerase chain reaction (PCR) chamber, sealing to preserve the sample during PCR cycling, and transfer of the PCR-products and on-chip formamide post-processing for the analysis of DNA fragments by capillary gel electrophoresis. This new valve differs from prior art polydimethylsiloxane (PDMS) valves in that the valve is not actuated externally by air-pressure or vacuum so that it simplifies a DNA analysis system by eliminating the need for an air-pressure or vacuum source, and off-cartridge solenoid valves, control circuit boards and software. Instead, the new valve is actuated by a thermal cycling peltier assembly integrated within the hardware instrument that tightly comes in contact with a microfluidic cartridge for thermal activation during PCR, so that it spontaneously closes the valve without an additional actuator system. The valve has bumps in the designated locations so that it has a self-alignment that does not require precise alignment of a valve actuator. Moreover, the thickness of the new valve is around 600 μm with an additional bump height of 400 μm so that it is easy to handle and very feasible to fabricate by injection molding compared to other PDMS valves whose thicknesses are around 30-100 μm. The new valve provided over 95% of metering performance in filling the fixed volume of the PCR chamber, preserved over 97% of the sample volume during PCR, and showed very comparable capillary electrophoresis peak heights to the benchtop assay tube controls with very consistent transfer volume of the PCR-product and on-chip formamide. The new valve can perform a core function for integrated nucleic acid analysis by capillary electrophoresis.

Risk factors of recurrence of macular oedema associated with branch retinal vein occlusion after intravitreal bevacizumab injection.

PubMed

Yoo, Jun Ho; Ahn, Jaemoon; Oh, Jaeryung; Cha, Jaehyung; Kim, Seong-Woo

2017-10-01

To identify risk factors of recurrence of macular oedema in branch retinal vein occlusion (BRVO) after intravitreal bevacizumab (IVB) injection. The records of 63 patients who underwent IVB injection for macular oedema secondary to BRVO with at least 6 months of follow-up were reviewed. Patients were evaluated at baseline with fluorescein angiography (FA), optical coherence tomography (OCT) and ultra-wide-field fundus photography (WFP). During follow-up, OCT and WFP were repeated. The area of retinal haemorrhage, central retinal thickness (CRT), area (mm 2 ) of capillary non-perfusion within the 1 mm (NPA1), 1-3 mm and 6 mm zones of the ETDRS circle, foveal capillary filling time, degree (°) of foveal capillary network destruction and FA pattern were analysed. Macular oedema recurred in 41 of 63 (65.1%) eyes after initial IVB injection. A binary logistic regression model showed that NPA1 (OR=434.97; 95% CI=5.52 to 34262.12, p=0.006) and initial CRT (OR=1.004; 95% CI=1.000 to 1.008, p=0.015) were significantly associated with the recurrence of macular oedema. Receiver operating characteristic curve analysis identified an NPA1 of 0.36 mm 2 (AUC: 0.735, sensitivity: 70.7%; specificity: 63.6%) and an initial CRT of 570 µm (AUC: 0.745, sensitivity: 63.4%; specificity: 77.3%) as cut-off values for predicting recurrence of macular oedema. Patients with BRVO with non-perfusion of more than half of the 1 mm zone of the ETDRS circle or with an initial CRT >570 µm should be closely monitored for macular oedema recurrence within 6 months of IVB injection. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Capillary flow of amorphous metal for high performance electrode

PubMed Central

Kim, Se Yun; Kim, Suk Jun; Jee, Sang Soo; Park, Jin Man; Park, Keum Hwan; Park, Sung Chan; Cho, Eun Ae; Lee, Jun Ho; Song, In Yong; Lee, Sang Mock; Han, In Taek; Lim, Ka Ram; Kim, Won Tae; Park, Ju Cheol; Eckert, Jürgen; Kim, Do Hyang; Lee, Eun-Sung

2013-01-01

Metallic glass (MG) assists electrical contact of screen-printed silver electrodes and leads to comparable electrode performance to that of electroplated electrodes. For high electrode performance, MG needs to be infiltrated into nanometer-scale cavities between Ag particles and reacts with them. Here, we show that the MG in the supercooled state can fill the gap between Ag particles within a remarkably short time due to capillary effect. The flow behavior of the MG is revealed by computational fluid dynamics and density funtional theory simulation. Also, we suggest the formation mechanism of the Ag electrodes, and demonstrate the criteria of MG for higher electrode performance. Consequently, when Al85Ni5Y8Co2 MG is added in the Ag electrodes, cell efficiency is enhanced up to 20.30% which is the highest efficiency reported so far for screen-printed interdigitated back contact solar cells. These results show the possibility for the replacement of electroplating process to screen-printing process. PMID:23851671

Extreme degree of ionization in homogenous micro-capillary plasma columns heated by ultrafast current pulses.

PubMed

Avaria, G; Grisham, M; Li, J; Tomasel, F G; Shlyaptsev, V N; Busquet, M; Woolston, M; Rocca, J J

2015-03-06

Homogeneous plasma columns with ionization levels typical of megaampere discharges are created by rapidly heating gas-filled 520-μm-diameter channels with nanosecond rise time current pulses of 40 kA. Current densities of up to 0.3  GA cm^{-2} greatly increase Joule heating with respect to conventional capillary discharge Z pinches, reaching unprecedented degrees of ionization for a high-Z plasma column heated by a current pulse of remarkably low amplitude. Dense xenon plasmas are ionized to Xe^{28+}, while xenon impurities in hydrogen discharges reach Xe^{30+}. The unique characteristics of these hot, ∼300:1 length-to-diameter aspect ratio plasmas allow the observation of unexpected spectroscopic phenomena. Axial spectra show the unusual dominance of the intercombination line over the resonance line of He-like Al by nearly an order of magnitude, caused by differences in opacities in the axial and radial directions. These plasma columns could enable the development of sub-10-nm x-ray lasers.

Counting polymers moving through a single ion channel

NASA Astrophysics Data System (ADS)

Bezrukov, Sergey M.; Vodyanoy, Igor; Parsegian, V. Adrian

1994-07-01

THE change in conductance of a small electrolyte-filled capillary owing to the passage of sub-micrometre-sized particles has long been used for particle counting and sizing. A commercial device for such measurements, the Coulter counter, is able to detect particles of sizes down to several tenths of a micrometre1-3. Nuclepore technology (in which pores are etched particle tracks) has extended the lower limit of size detection to 60-nm particles by using a capillary of diameter 0.45 μm (ref. 4). Here we show that natural channel-forming peptides incorporated into a bilayer lipid membrane can be used to detect the passage of single molecules with gyration radii as small as 5-15 Å. From our experiments with alamethicin pores we infer both the average number and the diffusion coefficients of poly(ethylene glycol) molecules in the pore. Our approach provides a means of observing the statistics and mechanics of flexible polymers moving within the confines of precisely defined single-molecule structures.

Modeling of the anode of a liquid-feed DMFC: Inhomogeneous compression effects and two-phase transport phenomena

NASA Astrophysics Data System (ADS)

García-Salaberri, Pablo A.; Vera, Marcos; Iglesias, Immaculada

2014-01-01

An isothermal two-phase 2D/1D across-the-channel model for the anode of a liquid-feed Direct Methanol Fuel Cell (DMFC) is presented. The model takes into account the effects of the inhomogeneous assembly compression of the Gas Diffusion Layer (GDL), including the spatial variations of porosity, diffusivity, permeability, capillary pressure, and electrical conductivity. The effective anisotropic properties of the GDL are evaluated from empirical data reported in the literature corresponding to Toray carbon paper TGP-H series. Multiphase transport is modeled according to the classical theory of porous media (two-fluid model), considering the effect of non-equilibrium evaporation and condensation of methanol and water. The numerical results evidence that the hydrophobic Leverett J-function approach is physically inconsistent to describe capillary transport in the anode of a DMFC when assembly compression effects are considered. In contrast, more realistic results are obtained when GDL-specific capillary pressure curves reflecting the mixed-wettability characteristics of GDLs are taken into account. The gas coverage factor at the GDL/channel interface also exhibits a strong influence on the gas-void fraction distribution in the GDL, which in turn depends on the relative importance between the capillary resistance induced by the inhomogeneous compression, Rc(∝ ∂pc / ∂ ε) , and the capillary diffusivity, Dbarc(∝ ∂pc / ∂ s) .

Using passive capillary lysimeter water flux measurements to improve flow predictions in variably saturated soils.

USDA-ARS?s Scientific Manuscript database

Passive capillary lysimeters (PCLs) are uniquely suited for measuring water fluxes in variably-saturated soils. The objective of this work was to compare PCL flux measurements with simulated fluxes obtained with a calibrated unsaturated flow model. The Richards equation-based model was calibrated us...

Quantifying water flow and retention in an unsaturated fracture-facial domain

USGS Publications Warehouse

Nimmo, John R.; Malek-Mohammadi, Siamak

2015-01-01

Hydrologically significant flow and storage of water occur in macropores and fractures that are only partially filled. To accommodate such processes in flow models, we propose a three-domain framework. Two of the domains correspond to water flow and water storage in a fracture-facial region, in addition to the third domain of matrix water. The fracture-facial region, typically within a fraction of a millimeter of the fracture wall, includes a flowing phase whose fullness is determined by the availability and flux of preferentially flowing water, and a static storage portion whose fullness is determined by the local matric potential. The flow domain can be modeled with the source-responsive preferential flow model, and the roughness-storage domain can be modeled with capillary relations applied on the fracture-facial area. The matrix domain is treated using traditional unsaturated flow theory. We tested the model with application to the hydrology of the Chalk formation in southern England, coherently linking hydrologic information including recharge estimates, streamflow, water table fluctuation, imaging by electron microscopy, and surface roughness. The quantitative consistency of the three-domain matrix-microcavity-film model with this body of diverse data supports the hypothesized distinctions and active mechanisms of the three domains and establishes the usefulness of this framework.

Modeling the interactions between compliant microcapsules and pillars in microchannels

NASA Astrophysics Data System (ADS)

Zhu, Guangdong; Alexeev, Alexander; Kumacheva, Eugenia; Balazs, Anna C.

2007-07-01

Using a computational model, we investigate the motion of microcapsules inside a microchannel that encompasses a narrow constriction. The microcapsules are composed of a compliant, elastic shell and an encapsulated fluid; these fluid-filled shells model synthetic polymeric microcapsules or biological cells (e.g., leukocytes). Driven by an imposed flow, the capsules are propelled along the microchannel and through the constricted region, which is formed by two pillars that lie in registry, extending from the top and bottom walls of the channels. The tops of these pillars (facing into the microchannel) are modified to exhibit either a neutral or an attractive interaction with the microcapsules. The pillars (and constriction) model topological features that can be introduced into microfluidic devices or the physical and chemical heterogeneities that are inherently present in biological vessels. To simulate the behavior of this complex system, we employ a hybrid method that integrates the lattice Boltzmann model (LBM) for fluid dynamics and the lattice spring model (LSM) for the micromechanics of elastic solids. Through this LBM/LSM technique, we probe how the capsule's stiffness and interaction with the pillars affect its passage through the chambers. The results yield guidelines for regulating the movement of microcarriers in microfluidic systems and provide insight into the flow properties of biological cells in capillaries.

Development of an Uncooled Photomechanic Infrared Sensor Based on the IR Organ of the Pyrophilous Jewel Beetle Melanophila Acuminata

DTIC Science & Technology

2007-01-04

been found to be a suitable means for depositing thin films of chitosan onto the surface of a silicon wafer. A small amount of liquid solution is...response of the system to changes of the meniscus position in the capillary. This was done by gradually filling the working volume with an ionic liquid ...from the wetting of the electrodes by the ionic liquid . From this data we have calculated a signal to noise ratio of 100 for the read out mechanism

Paramecium swimming in capillary tube

NASA Astrophysics Data System (ADS)

Jana, Saikat; Um, Soong Ho; Jung, Sunghwan

2012-04-01

Swimming organisms in their natural habitat need to navigate through a wide range of geometries and chemical environments. Interaction with boundaries in such situations is ubiquitous and can significantly modify the swimming characteristics of the organism when compared to ideal laboratory conditions. We study the different patterns of ciliary locomotion in glass capillaries of varying diameter and characterize the effect of the solid boundaries on the velocities of the organism. Experimental observations show that Paramecium executes helical trajectories that slowly transition to straight lines as the diameter of the capillary tubes decreases. We predict the swimming velocity in capillaries by modeling the system as a confined cylinder propagating longitudinal metachronal waves that create a finite pressure gradient. Comparing with experiments, we find that such pressure gradient considerations are necessary for modeling finite sized ciliary organisms in restrictive geometries.

A Theoretical Analysis of the Influence of Electroosmosis on the Effective Ionic Mobility in Capillary Zone Electrophoresis

ERIC Educational Resources Information Center

Hijnen, Hens

2009-01-01

A theoretical description of the influence of electroosmosis on the effective mobility of simple ions in capillary zone electrophoresis is presented. The mathematical equations derived from the space-charge model contain the pK[subscript a] value and the density of the weak acid surface groups as parameters characterizing the capillary. It is…

Nonlinear extension of a hemodynamic linear model for coherent hemodynamics spectroscopy.

PubMed

Sassaroli, Angelo; Kainerstorfer, Jana M; Fantini, Sergio

2016-01-21

In this work, we are proposing an extension of a recent hemodynamic model (Fantini, 2014a), which was developed within the framework of a novel approach to the study of tissue hemodynamics, named coherent hemodynamics spectroscopy (CHS). The previous hemodynamic model, from a signal processing viewpoint, treats the tissue microvasculature as a linear time-invariant system, and considers changes of blood volume, capillary blood flow velocity and the rate of oxygen diffusion as inputs, and the changes of oxy-, deoxy-, and total hemoglobin concentrations (measured in near infrared spectroscopy) as outputs. The model has been used also as a forward solver in an inversion procedure to retrieve quantitative parameters that assess physiological and biological processes such as microcirculation, cerebral autoregulation, tissue metabolic rate of oxygen, and oxygen extraction fraction. Within the assumption of "small" capillary blood flow velocity oscillations the model showed that the capillary and venous compartments "respond" to this input as low pass filters, characterized by two distinct impulse response functions. In this work, we do not make the assumption of "small" perturbations of capillary blood flow velocity by solving without approximations the partial differential equation that governs the spatio-temporal behavior of hemoglobin saturation in capillary and venous blood. Preliminary comparison between the linear time-invariant model and the extended model (here identified as nonlinear model) are shown for the relevant parameters measured in CHS as a function of the oscillation frequency (CHS spectra). We have found that for capillary blood flow velocity oscillations with amplitudes up to 10% of the baseline value (which reflect typical scenarios in CHS), the discrepancies between CHS spectra obtained with the linear and nonlinear models are negligible. For larger oscillations (~50%) the linear and nonlinear models yield CHS spectra with differences within typical experimental errors, but further investigation is needed to assess the effect of these differences. Flow oscillations larger than 10-20% are not typically induced in CHS; therefore, the results presented in this work indicate that a linear hemodynamic model, combined with a method to elicit controlled hemodynamic oscillations (as done for CHS), is appropriate for the quantitative assessment of cerebral microcirculation. Copyright © 2015 Elsevier Ltd. All rights reserved.

The role of the cerebral capillaries in acute ischemic stroke: the extended penumbra model.

PubMed

Østergaard, Leif; Jespersen, Sune Nørhøj; Mouridsen, Kim; Mikkelsen, Irene Klærke; Jonsdottír, Kristjana Ýr; Tietze, Anna; Blicher, Jakob Udby; Aamand, Rasmus; Hjort, Niels; Iversen, Nina Kerting; Cai, Changsi; Hougaard, Kristina Dupont; Simonsen, Claus Z; Von Weitzel-Mudersbach, Paul; Modrau, Boris; Nagenthiraja, Kartheeban; Riisgaard Ribe, Lars; Hansen, Mikkel Bo; Bekke, Susanne Lise; Dahlman, Martin Gervais; Puig, Josep; Pedraza, Salvador; Serena, Joaquín; Cho, Tae-Hee; Siemonsen, Susanne; Thomalla, Götz; Fiehler, Jens; Nighoghossian, Norbert; Andersen, Grethe

2013-05-01

The pathophysiology of cerebral ischemia is traditionally understood in relation to reductions in cerebral blood flow (CBF). However, a recent reanalysis of the flow-diffusion equation shows that increased capillary transit time heterogeneity (CTTH) can reduce the oxygen extraction efficacy in brain tissue for a given CBF. Changes in capillary morphology are typical of conditions predisposing to stroke and of experimental ischemia. Changes in capillary flow patterns have been observed by direct microscopy in animal models of ischemia and by indirect methods in humans stroke, but their metabolic significance remain unclear. We modeled the effects of progressive increases in CTTH on the way in which brain tissue can secure sufficient oxygen to meet its metabolic needs. Our analysis predicts that as CTTH increases, CBF responses to functional activation and to vasodilators must be suppressed to maintain sufficient tissue oxygenation. Reductions in CBF, increases in CTTH, and combinations thereof can seemingly trigger a critical lack of oxygen in brain tissue, and the restoration of capillary perfusion patterns therefore appears to be crucial for the restoration of the tissue oxygenation after ischemic episodes. In this review, we discuss the possible implications of these findings for the prevention, diagnosis, and treatment of acute stroke.

Laws of physics help explain capillary non-perfusion in diabetic retinopathy.

PubMed

Stefánsson, E; Chan, Y K; Bek, T; Hardarson, S H; Wong, D; Wilson, D I

2018-02-01

The purpose is to use laws of physics to elucidate the mechanisms behind capillary non-perfusion in diabetic retinopathy. In diabetic retinopathy, loss of pericytes weakens capillary walls and the vessel dilates. A dilated capillary has reduced resistance to flow, therefore increased flow in that vessel and decreased in adjoining capillaries. A preferential shunt vessel is thus formed from the dilated capillary and the adjacent capillaries become non-perfused. We apply the laws of Laplace and Hagen-Poiseuille to better understand the phenomena that lead to capillary non-perfusion. These laws of physics can give a foundation for physical or mathematical models to further elucidate this field of study. The law of Laplace predicts that a weaker vessel wall will dilate, assuming constant transmural pressure. The Hagen-Poiseuille equation for flow and the Ostwald-de Waele relationship for viscosity predict that a dilated vessel will receive a higher portion of the fluid flow than the adjoining capillaries. Viscosity will decrease in the dilated vessel, furthering the imbalance and resulting in a patch of non-perfused capillaries next to the dilated 'preferential' shunt vessel. Physical principles support or inspire novel hypotheses to explain poorly understood phenomena in ophthalmology. This thesis of pericyte death and capillary remodelling, which was first proposed by Cogan and Kuwabara, already agrees with histological and angiographical observations in diabetic retinopathy. We have shown that it is also supported by classical laws of physics.

Computer-aided design of microvasculature systems for use in vascular scaffold production.

PubMed

Mondy, William Lafayette; Cameron, Don; Timmermans, Jean-Pierre; De Clerck, Nora; Sasov, Alexander; Casteleyn, Christophe; Piegl, Les A

2009-09-01

In vitro biomedical engineering of intact, functional vascular networks, which include capillary structures, is a prerequisite for adequate vascular scaffold production. Capillary structures are necessary since they provide the elements and compounds for the growth, function and maintenance of 3D tissue structures. Computer-aided modeling of stereolithographic (STL) micro-computer tomographic (micro-CT) 3D models is a technique that enables us to mimic the design of vascular tree systems containing capillary beds, found in tissues. In our first paper (Mondy et al 2009 Tissue Eng. at press), using micro-CT, we studied the possibility of using vascular tissues to produce data capable of aiding the design of vascular tree scaffolding, which would help in the reverse engineering of a complete vascular tree system including capillary bed structures. In this paper, we used STL models of large datasets of computer-aided design (CAD) data of vascular structures which contained capillary structures that mimic those in the dermal layers of rabbit skin. Using CAD software we created from 3D STL models a bio-CAD design for the development of capillary-containing vascular tree scaffolding for skin. This method is designed to enhance a variety of therapeutic protocols including, but not limited to, organ and tissue repair, systemic disease mediation and cell/tissue transplantation therapy. Our successful approach to in vitro vasculogenesis will allow the bioengineering of various other types of 3D tissue structures, and as such greatly expands the potential applications of biomedical engineering technology into the fields of biomedical research and medicine.

Flow Analysis of Isobutane (R-600A) Inside AN Adiabatic Capillary Tube

NASA Astrophysics Data System (ADS)

Alok, Praveen; Sahu, Debjyoti

2018-02-01

Capillary tubes are simple narrow tubes but the phase change which occurs inside the capillary tubes is complex to analyze. In the present investigation, an attempt is made to analyze the flow of Isobutane (R-600a) inside the coiled capillary tubes for different load conditions by Homogeneous Equilibrium Model. The Length and diameter of the capillary tube not only depend on the pressure and temperature of the condenser and evaporator but also on the cooling load. The present paper investigates the change in dimensions of the coil capillary tube with respect to the change in cooling load on the system for the constant condenser and evaporator conditions. ANSYS CFX (Central Florida Expressway) software is used to study the flow characteristics of the refrigerant. Appropriate helical coil is selected for this analysis.

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers

NASA Astrophysics Data System (ADS)

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. [Figure not available: see fulltext.

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers.

PubMed

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. Graphical Abstract ᅟ.

Exploring the effect of nested capillaries on core-cladding mode resonances in hollow-core antiresonant fibers

NASA Astrophysics Data System (ADS)

Provino, Laurent; Taunay, Thierry

2018-02-01

Optimal suppression of higher-order modes (HOMs) in hollow-core antiresonant fibers comprising a single ring of thin-walled capillaries was previously studied, and can be achieved when the condition on the capillary-tocore diameter ratio is satisfied (d/D ≍ 0.68). Here we report on the conditions for maximizing the leakage losses of HOMs in hollow-core nested antiresonant node-less fibers, while preserving low confinement loss for the fundamental mode. Using an analytical model based on coupled capillary waveguides, as well as full-vector finite element modeling, we show that optimal d/D value leading to high leakage losses of HOMs, is strongly correlated to the size of nested capillaries. We also show that extremely high value of degree of HOM suppression (˜1200) at the resonant coupling is almost unchanged on a wide range of nested capillary diameter dN ested values. These results thus suggest the possibility of designing antiresonant fibers with nested elements, which show optimal guiding performances in terms of the HOM loss compared to that of the fundamental mode, for clearly defined paired values of the ratios dN ested/d and d/D. These can also tend towards a single-mode behavior only when the dimensionless parameter dN ested/d is less than 0.30, with identical wall thicknesses for all of the capillaries.

Pleural pressure theory revisited: a role for capillary equilibrium.

PubMed

Casha, Aaron R; Caruana-Gauci, Roberto; Manche, Alexander; Gauci, Marilyn; Chetcuti, Stanley; Bertolaccini, Luca; Scarci, Marco

2017-04-01

Theories elucidating pleural pressures should explain all observations including the equal and opposite recoil of the chest wall and lungs, the less than expected pleural hydrostatic gradient and its variation at lobar margins, why pleural pressures are negative and how pleural fluid circulation functions. A theoretical model describing equilibrium between buoyancy, hydrostatic forces, and capillary forces is proposed. The capillary equilibrium model described depends on control of pleural fluid volume and protein content, powered by an active pleural pump. The interaction between buoyancy forces, hydrostatic pressure and capillary pressure was calculated, and values for pleural thickness and pressure were determined using values for surface tension, contact angle, pleural fluid and lung densities found in the literature. Modelling can explain the issue of the differing hydrostatic vertical pleural pressure gradient at the lobar margins for buoyancy forces between the pleural fluid and the lung floating in the pleural fluid according to Archimedes' hydrostatic paradox. The capillary equilibrium model satisfies all salient requirements for a pleural pressure model, with negative pressures maximal at the apex, equal and opposite forces in the lung and chest wall, and circulatory pump action. This model predicts that pleural effusions cannot occur in emphysema unless concomitant heart failure increases lung density. This model also explains how the non-confluence of the lung with the chest wall (e.g., lobar margins) makes the pleural pressure more negative, and why pleural pressures would be higher after an upper lobectomy compared to a lower lobectomy. Pathological changes in pleural fluid composition and lung density alter the equilibrium between capillarity and buoyancy hydrostatic pressure to promote pleural effusion formation.

Effect of surrounding vasculature on intravoxel BOLD signal.

PubMed

Chen, Zikuan; Caprihan, Arvind; Calhoun, Vince

2010-04-01

The nonlocal influence from distant magnetization will affect the magnetic field at a voxel in question. Existing reports on BOLD simulation only consider vasculature inside a single voxel, thus omitting the contribution from the surrounding regions. In this article, the authors study the effect of the surrounding vasculature on the magnetic field and the BOLD signal at a cortical voxel by numerical simulation. A cortical voxel is generated as a cubic bin filled with randomly networked capillary vessels. First, the authors generate a cortical voxel with a random vessel network and embed it in a greater voxel by filling its surrounding region with vasculatures by different strategies. Next, they calculate the blood-susceptibility-induced magnetic field (BOLD field) at the voxel of interest (VOI) by a Fourier transform technique for different surrounding scenarios and varying surrounding extent. The BOLD field inhomogeneity is described by a radial distribution with a collection of cubic shell masks. The surrounding extent is defined by a collection of concentric cubes, which encase the VOI. Given a BOLD field in the presence of surrounding vasculature, they calculate BOLD signals by intravoxel dephasing. The influence from the surroundings on the BOLD field at a voxel in question mainly happens at the boundary. The most influence to the BOLD signal is from the inner surroundings. For a 160 x 160 x 160 microm3 voxel embedded in a 480 x 480 x 480 microm3 greater region, the surroundings could disturb the magnetic field by an amount in the range of [-0.002, 0.010] ppmT and could change the BOLD signal ratio in the range of [2.5%, 10%]. (These results were generated from the setting of delta(chi b)B0 = 3 ppmT, capillary = {2.5,6,9} microm, and relaxation time = 60 ms). The surrounding vasculature will impose a magnetic field disturbance at the voxel in question due to the nonlocal influence of magnetization. Simulation results show that the surrounding vasculature significantly alters the magnetic field (up to 0.01 ppmT) and BOLD signal (typically no more than 10%) at the central voxel and thus should be considered in accurate BOLD modeling.

A model and numerical method for compressible flows with capillary effects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmidmayer, Kevin, E-mail: kevin.schmidmayer@univ-amu.fr; Petitpas, Fabien, E-mail: fabien.petitpas@univ-amu.fr; Daniel, Eric, E-mail: eric.daniel@univ-amu.fr

2017-04-01

A new model for interface problems with capillary effects in compressible fluids is presented together with a specific numerical method to treat capillary flows and pressure waves propagation. This new multiphase model is in agreement with physical principles of conservation and respects the second law of thermodynamics. A new numerical method is also proposed where the global system of equations is split into several submodels. Each submodel is hyperbolic or weakly hyperbolic and can be solved with an adequate numerical method. This method is tested and validated thanks to comparisons with analytical solutions (Laplace law) and with experimental results onmore » droplet breakup induced by a shock wave.« less

Analysis of anabolic androgenic steroids in urine by full-capillary sample injection combined with a sweeping CE stacking method.

PubMed

Wang, Chun-Chi; Cheng, Shu-Fang; Cheng, Hui-Ling; Chen, Yen-Ling

2013-02-01

This study describes an on-line stacking CE approach by sweeping with whole capillary sample filling for analyzing five anabolic androgenic steroids in urine samples. The five anabolic steroids for detection were androstenedione, testosterone, epitestosterone, boldenone, and clostebol. Anabolic androgenic steroids are abused in sport doping because they can promote muscle growth. Therefore, a sensitive detection method is imperatively required for monitoring the urine samples of athletes. In this research, an interesting and reliable stacking capillary electrophoresis method was established for analysis of anabolic steroids in urine. After liquid-liquid extraction by n-hexane, the supernatant was dried and reconstituted with 30 mM phosphate buffer (pH 5.00) and loaded into the capillary by hydrodynamic injection (10 psi, 99.9 s). The stacking and separation were simultaneously accomplished at -20 kV in phosphate buffer (30 mM, pH 5.0) containing 100 mM sodium dodecyl sulfate and 40 % methanol. During the method validation, calibration curves were linear (r≥0.990) over a range of 50-1,000 ng/mL for the five analytes. In the evaluation of precision and accuracy for this method, the absolute values of the RSD and the RE in the intra-day (n=3) and inter-day (n=5) analyses were all less than 6.6 %. The limit of detection for the five analytes was 30 ng/mL (S/N=5, sampling 99.9 s at 10 psi). Compared with simple MECK, this stacking method possessed a 108- to 175-fold increase in sensitivity. This simple and sensitive stacking method could be used as a powerful tool for monitoring the illegal use of doping.

Determining bruise etiology in muscle tissue using finite element analysis.

PubMed

Tang, Kevin; Sharpe, Wyatt; Schulz, Alexandra; Tam, Edric; Grosse, Ian; Tis, John; Cullinane, Dennis

2014-03-01

Bruising, the result of capillary failure, is a common physical exam finding due to blunt trauma and, depending on location and severity, a potential indicator of abuse. Despite its clinical relevance, few studies have investigated the etiology of capillary failure. The goal of this study was to determine whether capillaries primarily fail under shear stress or hydraulic-induced tensile stress. An arteriole bifurcating into four capillaries was modeled using ANSYS 14.0 (®) . The capillaries were embedded in muscle tissue and a pressure of 20.4 kPa was applied. Any tensile stress exceeding 8.4 × 10(4)  Pa was considered failure. Results showed that failure occurred directly under the impact zone and where capillaries bifurcated, rather than along the line of greatest shear stress, indicating that internal tensile stress is likely the primary mode of capillary failure in bruising. These results are supported by the concept that bruising can occur via blunt trauma in which no shearing lacerations occur. © 2013 American Academy of Forensic Sciences.

Effects of anesthesia on the cerebral capillary blood flow in young and old mice

NASA Astrophysics Data System (ADS)

Moeini, Mohammad; Tabatabaei, Maryam S.; Bélanger, Samuel; Avti, Pramod; Castonguay, Alexandre; Pouliot, Philippe; Lesage, Frédéric

2015-03-01

Despite recent findings on the possible role of age-related cerebral microvasculature changes in cognition decline, previous studies of capillary blood flow in aging (using animal models) are scarce and limited to anesthetized conditions. Since anesthesia can have different effects in young and old animals, it may introduce a confounding effect in aging studies. The present study aimed to eliminate the potential confound introduced by anesthesia by measuring capillary blood flow parameters in both awake conditions and under isoflurane anesthesia. We used 2-photon laser scanning fluorescence microscopy to measure capillary diameter, red blood cell velocity and flux, hematocrit and capillary volumetric flow in individual capillaries in the barrel cortex of 6- and 24-month old C57Bl/6 mice. It was observed that microvascular properties are significantly affected by anesthesia leading to different trends in capillary blood flow parameters with aging when measured under awake or anesthetized conditions. The findings in this study suggest taking extra care in interpreting aging studies from anesthetized animals.

Effect of anti-vertigo granule on the opening number and blood flow of mouse ear capillary network

NASA Astrophysics Data System (ADS)

Li, Chongxian; Liu, Xiaobin; Li, Jun; Hao, Shaojun; Wang, Xidong; Li, Wenjun; Zhang, Zhengchen

2018-04-01

To observe the effects of anti-glare particles on the open number and blood flow in the auricle of mice with microcirculation disturbance model. Sixty mice, half male and half female, were randomly divided into 6 groups. The mice were given Kangxuan granule suspension, serum brain granule suspension and normal saline of the same volume, respectively, once a day. The mice were anesthetized by intraperitoneal injection of chloral hydrate at 1 hour after the last administration. The mouse was fixed on the observation platform and the auricle was placed on the transmission stage. BZ-2000 microcirculation microscope and microcirculation analysis system were used to observe the changes of blood velocity and capillary opening volume in auricle of mice before administration. The changes of blood velocity and capillaries opening volume of mouse auricle were observed 2 min after epinephrine injection into tail vein of mice. Bear fruit: Compared with those before epinephrine, the opening number of capillary reticulum of auricle in large dose Kangxuan granule group was significantly decreased (P<0.05), and in normal saline group and middle group. In the small dose Kangxuan granule group, the opening number of capillary network of auricle decreased significantly (P<0.01). Compared with the model group, the large dose Kangxuan granule group could significantly increase the opening number of the auricle capillary network in mice (P<0.01). Yangxuannao granule group could significantly increase the opening number of auricle capillary reticulum in mice (P<0.05), compared with the model group by Ridit test. Both Kangxuan granule group and Yangxuannao granule group could significantly improve the auricle hair of mice with microcirculation disorder. The blood flow in fine blood vessels (P<0.01). Kangxuan granule has a good effect on the opening number of capillary network of auricle and blood flow in mice with microcirculation disorder.

Screening HIV-1 fusion inhibitors based on capillary electrophoresis head-end microreactor targeting to the core structure of gp41.

PubMed

Liu, Lihong; Xu, Xiaoying; Liu, Yanhui; Zhang, Xuanxuan; Li, Lin; Jia, Zhimin

2016-02-20

In this paper, we design a microreactor based on electrophoretically mediated microanalysis (EMMA) with capillary electrophoresis (CE) for screening HIV-1 inhibitors that bind to the N-terminal heptad repeat (NHR, N36) region. Initially, a test sample plug is loaded into a capillary filled with buffer solution followed by N36 peptide solution, and the two solutions simultaneously mix by diffusion. Then, voltage is applied, and the sample molecules pass through the N36 peptide zone. The active compounds combine with N36, leading to a loss in the peak height of the active compound. More than 100 traditional Chinese medicine extracts (TCME) were screened, and an extract of Pheretima aspergillum (E. Perrier) (L5) was identified as having potent inhibitory activity. The results showed that L5 could significantly inhibit the HIV-1JR-FL pseudotyped virus infection; the 50% effective concentration (EC50) of L5 was approximately 32.1±1.2μg/mL, and the 50% cytotoxicity concentration (CC50) value of L5 was 146.9±4.4μg/mL, suggesting that L5 had low in vitro cytotoxicity on U87-CD4-CCR5 cells. The new method is simple and rapid, is free of antibodies, and does not require tedious processes. Copyright © 2015 Elsevier B.V. All rights reserved.

Inert gas clearance from tissue by co-currently and counter-currently arranged microvessels

PubMed Central

Lu, Y.; Michel, C. C.

2012-01-01

To elucidate the clearance of dissolved inert gas from tissues, we have developed numerical models of gas transport in a cylindrical block of tissue supplied by one or two capillaries. With two capillaries, attention is given to the effects of co-current and counter-current flow on tissue gas clearance. Clearance by counter-current flow is compared with clearance by a single capillary or by two co-currently arranged capillaries. Effects of the blood velocity, solubility, and diffusivity of the gas in the tissue are investigated using parameters with physiological values. It is found that under the conditions investigated, almost identical clearances are achieved by a single capillary as by a co-current pair when the total flow per tissue volume in each unit is the same (i.e., flow velocity in the single capillary is twice that in each co-current vessel). For both co-current and counter-current arrangements, approximate linear relations exist between the tissue gas clearance rate and tissue blood perfusion rate. However, the counter-current arrangement of capillaries results in less-efficient clearance of the inert gas from tissues. Furthermore, this difference in efficiency increases at higher blood flow rates. At a given blood flow, the simple conduction-capacitance model, which has been used to estimate tissue blood perfusion rate from inert gas clearance, underestimates gas clearance rates predicted by the numerical models for single vessel or for two vessels with co-current flow. This difference is accounted for in discussion, which also considers the choice of parameters and possible effects of microvascular architecture on the interpretation of tissue inert gas clearance. PMID:22604885

Development of a flow controller for long-term sampling of gases and vapors using evacuated canisters.

PubMed

Rossner, Alan; Farant, Jean Pierre; Simon, Philippe; Wick, David P

2002-11-15

Anthropogenic activities contribute to the release of a wide variety of volatile organic compounds (VOC) into microenvironments. Developing and implementing new air sampling technologies that allow for the characterization of exposures to VOC can be useful for evaluating environmental and health concerns arising from such occurrences. A novel air sampler based on the use of a capillary flow controller connected to evacuated canisters (300 mL, 1 and 6 L) was designed and tested. The capillary tube, used to control the flow of air, is a variation on a sharp-edge orifice flow controller. It essentially controls the velocity of the fluid (air) as a function of the properties of the fluid, tube diameter and length. A model to predict flow rate in this dynamic system was developed. The mathematical model presented here was developed using the Hagen-Poiseuille equation and the ideal gas law to predict flow into the canisters used to sample for long periods of time. The Hagen-Poiseuille equation shows the relationship between flow rate, pressure gradient, capillary resistance, fluid viscosity, capillary length and diameter. The flow rates evaluated were extremely low, ranging from 0.05 to 1 mL min(-1). The model was compared with experimental results and was shown to overestimate the flow rate. Empirical equations were developed to more accurately predict flow for the 300 mL, 1 and 6 L canisters used for sampling periods ranging from several hours to one month. The theoretical and observed flow rates for different capillary geometries were evaluated. Each capillary flow controller geometry that was tested was found to generate very reproducible results, RSD < 2%. Also, the empirical formulas developed to predict flow rate given a specified diameter and capillary length were found to predict flow rate within 6% of the experimental data. The samplers were exposed to a variety of airborne vapors that allowed for comparison of the effectiveness of capillary flow controllers to sorbent samplers and to an online gas chromatograph. The capillary flow controller was found to exceed the performance of the sorbent samplers in this comparison.

Computation of three-phase capillary entry pressures and arc menisci configurations in pore geometries from 2D rock images: A combinatorial approach

NASA Astrophysics Data System (ADS)

Zhou, Yingfang; Helland, Johan Olav; Hatzignatiou, Dimitrios G.

2014-07-01

We present a semi-analytical, combinatorial approach to compute three-phase capillary entry pressures for gas invasion into pore throats with constant cross-sections of arbitrary shapes that are occupied by oil and/or water. For a specific set of three-phase capillary pressures, geometrically allowed gas/oil, oil/water and gas/water arc menisci are determined by moving two circles in opposite directions along the pore/solid boundary for each fluid pair such that the contact angle is defined at the front circular arcs. Intersections of the two circles determine the geometrically allowed arc menisci for each fluid pair. The resulting interfaces are combined systematically to allow for all geometrically possible three-phase configuration changes. The three-phase extension of the Mayer and Stowe - Princen method is adopted to calculate capillary entry pressures for all determined configuration candidates, from which the most favorable gas invasion configuration is determined. The model is validated by comparing computed three-phase capillary entry pressures and corresponding fluid configurations with analytical solutions in idealized triangular star-shaped pores. It is demonstrated that the model accounts for all scenarios that have been analyzed previously in these shapes. Finally, three-phase capillary entry pressures and associated fluid configurations are computed in throat cross-sections extracted from segmented SEM images of Bentheim sandstone. The computed gas/oil capillary entry pressures account for the expected dependence of oil/water capillary pressure in spreading and non-spreading fluid systems at the considered wetting conditions. Because these geometries are irregular and include constrictions, we introduce three-phase displacements that have not been identified previously in pore-network models that are based on idealized pore shapes. However, in the limited number of pore geometries considered in this work, we find that the favorable displacements are not generically different from those already encountered in network models previously, except that the size and shape of oil layers that are surrounded by gas and water are described more realistically. The significance of the results for describing oil connectivity in porous media accurately can only be evaluated by including throats with more complex cross-sections in three-phase pore-network models.

Experiment K-7-28: Lung Morphology Study

NASA Technical Reports Server (NTRS)

West, J. B.; Elliott, A. R.; Mathieu-Costello, O.; Kaplansky, A. S.

1994-01-01

There are no previous studies investigating the effect of microgravity exposure during spaceflight on lung tissue. We examined the ultrastructure of the left lungs of 5 Czechoslovakian Wistar rats flown on the 13 day, 19+ hr. Cosmos 2044 mission, and compared them to 5 vivarium and 5 synchronous controls at 1-g conditions, and 5 rats exposed to 14 days of tail-suspension. Within 10 minutes of sacrifice by decapitation, the lungs were removed and immersed in 3% glutaraldehyde in 0.1M phosphate buffer (total osmolarity of the fixative: 560 mOsm; pH = 7.4). The tissue stored at 5 C was transported to our laboratory where it was processed for light and electron microscopy. No significant perivascular cuffing caused by interstitial edema was present in the tissue samples. Some of the flight, tail-suspended, and synchronous control rats showed alveolar edema, while vivarium controls did not. The pulmonary capillaries appeared to be more congested in the flight animals than in the other groups. This could be related to the increased hematocrit due to the microgravity exposure. In all 5 flight, 4 tail-suspended, and 3 synchronous rats, red blood cells (RBC) were present in the alveolar spaces. The RBC were either suspended free in the alveoli or observed lining the alveolar wall. The frequency of RBC lining the alveolar walls appeared greater in the dorsal (gravity non-dependent) than in ventral (gravity dependent) regions of the lung in these three animal groups. In 3 of the vivarium controls, a few RBC were found in the alveolar spaces. Intra-capillary fluid-filled vesicles were observed in the flight, tail-suspended and synchronous animals, but not in the vivarium controls. The formation of intra-capillary fluid-filled vesicles has been previously associated with pulmonary hypertension induced by high altitude exposure and mitral stenosis. In conclusion, pulmonary hemorrhage and alveolar edema of unknown origin occurred to a greater extent in the flight, tail-suspended, and synchronous control animals, and in the dorsal regions of the lung when compared to the vivarium controls. The etiology of these changes, which are possibly due to an increase in pulmonary vascular pressure, requires further investigation.

Effect of filling ratio and orientation on the thermal performance of closed loop pulsating heat pipe using ethanol

NASA Astrophysics Data System (ADS)

Rahman, Md. Lutfor; Chowdhury, Mehrin; Islam, Nawshad Arslan; Mufti, Sayed Muhammad; Ali, Mohammad

2016-07-01

Pulsating heat pipe (PHP) is a new, promising yet ambiguous technology for effective heat transfer of microelectronic devices where heat is carried by the vapor plugs and liquid slugs of the working fluid. The aim of this research paper is to better understand the operation of PHP through experimental investigations and obtain comparative results for different parameters. A series of experiments are conducted on a closed loop PHP (CLPHP) with 8 loops made of copper capillary tube of 2 mm inner diameter. Ethanol is taken as the working fluid. The operating characteristics are studied for the variation of heat input, filling ratio (FR) and orientation. The filling ratios are 40%, 50%, 60% and 70% based on its total volume. The orientations are 0° (vertical), 30°, 45° and 60°. The results clearly demonstrate the effect of filling ratio and inclination angle on the performance, operational stability and heat transfer capability of ethanol as working fluid of CLPHP. Important insight of the operational characteristics of CLPHP is obtained and optimum performance of CLPHP using ethanol is thus identified. Ethanol works best at 50-60%FR at wide range of heat inputs. At very low heat inputs, 40%FR can be used for attaining a good performance. Filling ratio below 40%FR is not suitable for using in CLPHP as it gives a low performance. The optimum performance of the device can be obtained at vertical position.

An Earth-Based Model of Microgravity Pulmonary Physiology

NASA Technical Reports Server (NTRS)

Hirschl, Ronald B.; Bull, Joseph L.; Grothberg, James B.

2004-01-01

There are currently only two practical methods of achieving micro G for experimentation: parabolic flight in an aircraft or space flight, both of which have limitations. As a result, there are many important aspects of pulmonary physiology that have not been investigated in micro G. We propose to develop an earth-based animal model of micro G by using liquid ventilation, which will allow us to fill the lungs with perfluorocarbon, and submersing the animal in water such that the density of the lungs is the same as the surrounding environment. By so doing, we will eliminate the effects of gravity on respiration. We will first validate the model by comparing measures of pulmonary physiology, including cardiac output, central venous pressures, lung volumes, and pulmonary mechanics, to previous space flight and parabolic flight measurements. After validating the model, we will investigate the impact of micro G on aspects of lung physiology that have not been previously measured. These will include pulmonary blood flow distribution, ventilation distribution, pulmonary capillary wedge pressure, ventilation-perfusion matching, and pleural pressures and flows. We expect that this earth-based model of micro G will enhance our knowledge and understanding of lung physiology in space which will increase in importance as space flights increase in time and distance.

Process-morphology scaling relations quantify self-organization in capillary densified nanofiber arrays.

PubMed

Kaiser, Ashley L; Stein, Itai Y; Cui, Kehang; Wardle, Brian L

2018-02-07

Capillary-mediated densification is an inexpensive and versatile approach to tune the application-specific properties and packing morphology of bulk nanofiber (NF) arrays, such as aligned carbon nanotubes. While NF length governs elasto-capillary self-assembly, the geometry of cellular patterns formed by capillary densified NFs cannot be precisely predicted by existing theories. This originates from the recently quantified orders of magnitude lower than expected NF array effective axial elastic modulus (E), and here we show via parametric experimentation and modeling that E determines the width, area, and wall thickness of the resulting cellular pattern. Both experiments and models show that further tuning of the cellular pattern is possible by altering the NF-substrate adhesion strength, which could enable the broad use of this facile approach to predictably pattern NF arrays for high value applications.

Numerical Simulation of Sickle Cell Blood Flow in the Microcirculation

NASA Astrophysics Data System (ADS)

Berger, Stanley A.; Carlson, Brian E.

2001-11-01

A numerical simulation of normal and sickle cell blood flow through the transverse arteriole-capillary microcirculation is carried out to model the dominant mechanisms involved in the onset of vascular stasis in sickle cell disease. The transverse arteriole-capillary network is described by Strahler's network branching method, and the oxygen and blood transport in the capillaries is modeled by a Krogh cylinder analysis utilizing Lighthill's lubrication theory, as developed by Berger and King. Poiseuille's law is used to represent blood flow in the arterioles. Applying this flow and transport model and utilizing volumetric flow continuity at each network bifurcation, a nonlinear system of equations is obtained, which is solved iteratively using a steepest descent algorithm coupled with a Newton solver. Ten different networks are generated and flow results are calculated for normal blood and sickle cell blood without and with precapillary oxygen loss. We find that total volumetric blood flow through the network is greater in the two sickle cell blood simulations than for normal blood owing to the anemia associated with sickle cell disease. The percentage of capillary blockage in the network increases dramatically with decreasing pressure drop across the network in the sickle cell cases while there is no blockage when normal blood flows through simulated networks. It is concluded that, in sickle cell disease, without any vasomotor dilation response to decreasing oxygen concentrations in the blood, capillary blockage will occur in the microvasculature even at average pressure drops across the transverse arteriole-capillary networks.

Fast separation of enantiomers by capillary electrophoresis using a combination of two capillaries with different internal diameters.

PubMed

Šebestová, Andrea; Petr, Jan

2017-12-01

The combination of capillaries with different internal diameters was used to accelerate the separation of enantiomers in capillary electrophoresis. Separation of R,S-1,1'-binaphthalene-2,2'-diyl hydrogen phosphate using isopropyl derivative of cyclofructan 6 was studied as a model system. The best separation conditions included 500 mM sodium borate pH 9.5 with 60 mM concentration of the chiral selector. Separation lasted approx. 1.5 min using the combination of 50 and 100 μm id capillaries of 9.7 cm and 22.9 cm, respectively. It allowed approx. 12-fold acceleration in comparison to the traditional long-end separation mainly due to the higher electroosmotic flow generated in the connected capillaries. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Texas horned lizard as model for robust capillary structures for passive directional transport of cooling lubricants

NASA Astrophysics Data System (ADS)

Comanns, Philipp; Winands, Kai; Pothen, Mario; Bott, Raya A.; Wagner, Hermann; Baumgartner, Werner

2016-04-01

Moisture-harvesting lizards, such as the Texas horned lizard Phrynosoma cornutum, have remarkable adaptations for inhabiting arid regions. Special skin structures, in particular capillary channels in between imbricate overlapping scales, enable the lizard to collect water by capillarity and to transport it to the snout for ingestion. This fluid transport is passive and directional towards the lizard's snout. The directionality is based on geometric principles, namely on a periodic pattern of interconnected half-open capillary channels that narrow and widen. Following a biomimetic approach, these principles were transferred to technical prototype design and manufacturing. Capillary structures, 50 μm to 300 μm wide and approx. 70 μm deep, were realized by use of a pulsed picosecond laser in hot working tool steel, hardened to 52 HRC. In order to achieve highest functionality, strategies were developed to minimize potential structural inaccuracies, which can occur at the bottom of the capillary structures caused by the laser process. Such inaccuracies are in the range of 10 μm to 15 μm and form sub-capillary structures with greater capillary forces than the main channels. Hence, an Acceleration Compensation Algorithm was developed for the laser process to minimize or even avoid these inaccuracies. The capillary design was also identified to have substantial influence; by a hexagonal capillary network of non-parallel capillaries potential influences of sub-capillaries on the functionality were reduced to realize a robust passive directional capillary transport. Such smart surface structures can lead to improvements of technical systems by decreasing energy consumption and increasing the resource efficiency.

Heat transfer within a flat micro heat pipe with extra liquid

NASA Astrophysics Data System (ADS)

Sprinceana, Silviu; Mihai, Ioan

2016-12-01

In the real functioning of flat micro heat pipe (FMHP), there can appear cases when the temperature from the vaporization zone can exceed a critical value caused by a sudden increase of the thermal flow. The heat transfer which is completed conductively through the copper wall of a FMHP vaporizer causes the vaporization of the work fluid. On the condenser, the condensation of the fluid vapors and the transfer of the condenser to the vaporizer can no longer be achieved. The solution proposed for enhancing heat transfer in the event of blockage phenomenon FMHP, it is the injection of a certain amount of working fluid in the vaporization zone. By this process the working fluid injected into the evaporator passes suddenly in the vapor, producing a cooling zone. The new product additional mass of vapor will leave the vaporization zone and will condense in condensation zone, thereby supplementing the amount of condensation. Thus resumes normal operating cycle of FMHP. For the experimental measurements made for the transfer of heat through the FMHP working fluid demineralized water, they were made two micro-capillary tubes of sintered copper layer. The first was filled with 1ml of demineralized water was dropped under vacuum until the internal pressure has reached a level of 1•104Pa. The second FMHP was filled with the same amount of working fluid was used and the same capillary inner layer over which was laid a polysynthetic material that will accrue an additional amount of fluid. In this case, the internal pressure was reduced to 1•104Pa.

Dynamic indocyanine green angiography measurements

NASA Astrophysics Data System (ADS)

Holmes, Timothy; Invernizzi, Alessandro; Larkin, Sean; Staurenghi, Giovanni

2012-11-01

Dynamic indocyanine green imaging uses a scanning laser ophthalmoscope and a fluorescent dye to produce movies of the dye-filling pattern in the retina and choroid of the eye. It is used for evaluating choroidal neovascularization. Movies are examined to identify the anatomy of the pathology for planning treatment and to evaluate progression or response to treatment. The popularity of this approach is affected by the complexity and difficulty in interpreting the movies. Software algorithms were developed to produce images from the movies that are easy to interpret. A mathematical model is formulated of the flow dynamics, and a fitting algorithm is designed that solves for the flow parameters. The images provide information about flow and perfusion, including regions of change between examinations. Imaged measures include the dye fill-time, temporal dispersion, and magnitude of the dye dilution temporal curves associated with image pixels. Cases show how the software can help to identify clinically relevant anatomy such as feeder vessels, drain vessels, capillary networks, and normal choroidal draining vessels. As a potential tool for research into the character of neovascular conditions and treatments, it reveals the flow dynamics and character of the lesion. Future varieties of this methodology may be used for evaluating the success of engineered tissue transplants, surgical flaps, reconstructive surgery, breast surgery, and many other surgical applications where flow, perfusion, and vascularity of tissue are important.

A study of hearing function and histopathologic changes in the cochlea of the type 2 diabetes model Tsumura Suzuki obese diabetes mouse.

PubMed

Tsuda, Junko; Sugahara, Kazuma; Hori, Takeshi; Kanagawa, Eiju; Takaki, Eiichi; Fujimoto, Mitsuaki; Nakai, Akira; Yamashita, Hiroshi

2016-11-01

This study used Tsumura Suzuki Obese Diabetes (TSOD) mice as a spontaneous type 2 diabetes model and Tsumura Suzuki Non-obesity (TSNO) mice as controls to investigate factors involved in the onset of hearing impairment. Body weight, blood glucose levels, and auditory brainstem responses (ABRs) were measured. The cochleae were excised and evaluated histopathologically. The TSOD mice showed significant hyperglycemia at 2-7 months and severe obesity at 5-10 months; significantly elevated ABR thresholds at 8-10 months; and the capillary lumens in the cochlea stria vascularis were narrower in the TSOD mice than in the TSNO mice. At 17 months, India ink vascular staining of the TSOD mice's cochleae revealed decreased capillary density in the stria vascularis. The vascular area of capillaries in the stria vascularis and the vascular area were significantly smaller in TSOD mice. Histopathological analysis showed vessel wall thickening in the modiolus and narrowed capillaries in the stria vascularis, suggesting reduced blood flow to the inner ear. The diabetes mice model used in our study showed early age-associated hearing loss, and histopathology showed findings of vessel wall thickening in the modiolus, narrowing of capillaries in the stria vascularis, and chronically reduced blood flow in the cochlea.

The role of the cerebral capillaries in acute ischemic stroke: the extended penumbra model

PubMed Central

Østergaard, Leif; Jespersen, Sune Nørhøj; Mouridsen, Kim; Mikkelsen, Irene Klærke; Jonsdottír, Kristjana Ýr; Tietze, Anna; Blicher, Jakob Udby; Aamand, Rasmus; Hjort, Niels; Iversen, Nina Kerting; Cai, Changsi; Hougaard, Kristina Dupont; Simonsen, Claus Z; Von Weitzel-Mudersbach, Paul; Modrau, Boris; Nagenthiraja, Kartheeban; Riisgaard Ribe, Lars; Hansen, Mikkel Bo; Bekke, Susanne Lise; Dahlman, Martin Gervais; Puig, Josep; Pedraza, Salvador; Serena, Joaquín; Cho, Tae-Hee; Siemonsen, Susanne; Thomalla, Götz; Fiehler, Jens; Nighoghossian, Norbert; Andersen, Grethe

2013-01-01

The pathophysiology of cerebral ischemia is traditionally understood in relation to reductions in cerebral blood flow (CBF). However, a recent reanalysis of the flow-diffusion equation shows that increased capillary transit time heterogeneity (CTTH) can reduce the oxygen extraction efficacy in brain tissue for a given CBF. Changes in capillary morphology are typical of conditions predisposing to stroke and of experimental ischemia. Changes in capillary flow patterns have been observed by direct microscopy in animal models of ischemia and by indirect methods in humans stroke, but their metabolic significance remain unclear. We modeled the effects of progressive increases in CTTH on the way in which brain tissue can secure sufficient oxygen to meet its metabolic needs. Our analysis predicts that as CTTH increases, CBF responses to functional activation and to vasodilators must be suppressed to maintain sufficient tissue oxygenation. Reductions in CBF, increases in CTTH, and combinations thereof can seemingly trigger a critical lack of oxygen in brain tissue, and the restoration of capillary perfusion patterns therefore appears to be crucial for the restoration of the tissue oxygenation after ischemic episodes. In this review, we discuss the possible implications of these findings for the prevention, diagnosis, and treatment of acute stroke. PMID:23443173

C-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal.

PubMed

Feng, Hua; Wang, Fang; Wang, Changmiao

2016-07-01

To study the c-Kit expression in the gallbladder of cholesterol lithogenic guinea pig model and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal (ICCs). A total of 45 guinea pigs were randomly assigned into three groups: the control group (guinea pigs fed a standard diet, normal group); the model group (guinea pigs fed a cholesterol gallstone-inducing diet); and the Chinese medicine group (guinea pigs fed the cholesterol gallstone-inducing diet and treated with A. capillaris through intragastric administration, therapy group). Each group had 15 guinea pigs. The gallbladders of the guinea pigs were harvested after 8 weeks. C-Kit expression was detected using an immunohistochemistry staining, real-time PCR, and Western blot analyses. The effect of A. capillaris on ICCs was evaluated by muscle strip contraction experiments. C-Kit expression significantly decreased in the gallbladder of model group, but increased in the Chinese medicine group. The Contractility of guinea pig gallbladder muscle strip significantly improved in the Chinese medicine group. Our results indicated that A. capillaris improves gallbladder impairment by up-regulating c-Kit expression, and it also can improve the contractile response of in vitro guinea pig gallbladder muscle strips.

Hardening of particle/oil/water suspensions due to capillary bridges: Experimental yield stress and theoretical interpretation.

PubMed

Danov, Krassimir D; Georgiev, Mihail T; Kralchevsky, Peter A; Radulova, Gergana M; Gurkov, Theodor D; Stoyanov, Simeon D; Pelan, Eddie G

2018-01-01

Suspensions of colloid particles possess the remarkable property to solidify upon the addition of minimal amount of a second liquid that preferentially wets the particles. The hardening is due to the formation of capillary bridges (pendular rings), which connect the particles. Here, we review works on the mechanical properties of such suspensions and related works on the capillary-bridge force, and present new rheological data for the weakly studied concentration range 30-55 vol% particles. The mechanical strength of the solidified capillary suspensions, characterized by the yield stress Y, is measured at the elastic limit for various volume fractions of the particles and the preferentially wetting liquid. A quantitative theoretical model is developed, which relates Y with the maximum of the capillary-bridge force, projected on the shear plane. A semi-empirical expression for the mean number of capillary bridges per particle is proposed. The model agrees very well with the experimental data and gives a quantitative description of the yield stress, which increases with the rise of interfacial tension and with the volume fractions of particles and capillary bridges, but decreases with the rise of particle radius and contact angle. The quantitative description of capillary force is based on the exact theory and numerical calculation of the capillary bridge profile at various bridge volumes and contact angles. An analytical formula for Y is also derived. The comparison of the theoretical and experimental strain at the elastic limit reveals that the fluidization of the capillary suspension takes place only in a deformation zone of thickness up to several hundred particle diameters, which is adjacent to the rheometer's mobile plate. The reported experimental results refer to water-continuous suspension with hydrophobic particles and oily capillary bridges. The comparison of data for bridges from soybean oil and hexadecane surprisingly indicate that the yield strength is greater for the suspension with soybean oil despite its lower interfacial tension against water. The result can be explained with the different contact angles of the two oils in agreement with the theoretical predictions. The results could contribute for a better understanding, quantitative prediction and control of the mechanical properties of three-phase capillary suspensions solid/liquid/liquid. Copyright © 2017 Elsevier B.V. All rights reserved.

Limitations of the permeability-limited compartment model in estimating vascular permeability and interstitial volume fraction in DCE-MRI.

PubMed

Carreira, Guido Correia; Gemeinhardt, Ole; Gorenflo, Rudolf; Beyersdorff, Dirk; Franiel, Tobias; Plendl, Johanna; Lüdemann, Lutz

2011-06-01

Dynamic contrast-enhanced magnetic resonance imaging commonly uses compartment models to estimate tissue parameters in general and perfusion parameters in particular. Compartment models assume a homogeneous distribution of the injected tracer throughout the compartment volume. Since tracer distribution within a compartment cannot be assessed, the parameters obtained by means of a compartment model might differ from the actual physical values. This work systematically examines the widely used permeability-surface-limited one-compartment model to determine the reliability of the parameters obtained by comparing them with their actual values. A computer simulation was used to model spatial tracer distribution within the interstitial volume using diffusion of contrast agent in tissue. Vascular parameters were varied as well as tissue parameters. The vascular parameters used were capillary radius (4 and 12 μm), capillary permeability (from 0.03 to 3.3 μm/s) and intercapillary distances from 30 to 300 μm. The tissue parameters used were tortuosity (λ), porosity (α) and interstitial volume fraction (v(e)). Our results suggest that the permeability-surface-limited compartment model generally underestimates capillary permeability for capillaries with a radius of 4 μm by factors from ≈0.03 for α=0.04, to ≈ 0.1 for α=0.2, to ≈ 0.5 for α=1.0. An overestimation of actual capillary permeability for capillaries with a radius of 12 μm by a factor of ≥1.3 was found for α=1.0, while α=0.2 yielded an underestimation by a factor of ≈0.3 and α=0.04 by a factor of ≈ 0.03. The interstitial volume fraction, v(e), obtained by the compartment model differed with increasing intercapillary distances and for low vessel permeability, whereas v(e) was found to be estimated approximately accurately for P=0.3 μm/s and P=3.3 μm/s for vessel distances <100 μm. Copyright © 2011 Elsevier Inc. All rights reserved.

Pleural pressure theory revisited: a role for capillary equilibrium

PubMed Central

Caruana-Gauci, Roberto; Manche, Alexander; Gauci, Marilyn; Chetcuti, Stanley; Bertolaccini, Luca

2017-01-01

Background Theories elucidating pleural pressures should explain all observations including the equal and opposite recoil of the chest wall and lungs, the less than expected pleural hydrostatic gradient and its variation at lobar margins, why pleural pressures are negative and how pleural fluid circulation functions. Methods A theoretical model describing equilibrium between buoyancy, hydrostatic forces, and capillary forces is proposed. The capillary equilibrium model described depends on control of pleural fluid volume and protein content, powered by an active pleural pump. Results The interaction between buoyancy forces, hydrostatic pressure and capillary pressure was calculated, and values for pleural thickness and pressure were determined using values for surface tension, contact angle, pleural fluid and lung densities found in the literature. Modelling can explain the issue of the differing hydrostatic vertical pleural pressure gradient at the lobar margins for buoyancy forces between the pleural fluid and the lung floating in the pleural fluid according to Archimedes’ hydrostatic paradox. The capillary equilibrium model satisfies all salient requirements for a pleural pressure model, with negative pressures maximal at the apex, equal and opposite forces in the lung and chest wall, and circulatory pump action. Conclusions This model predicts that pleural effusions cannot occur in emphysema unless concomitant heart failure increases lung density. This model also explains how the non-confluence of the lung with the chest wall (e.g., lobar margins) makes the pleural pressure more negative, and why pleural pressures would be higher after an upper lobectomy compared to a lower lobectomy. Pathological changes in pleural fluid composition and lung density alter the equilibrium between capillarity and buoyancy hydrostatic pressure to promote pleural effusion formation. PMID:28523153

Capillary Contact Angle in a Completely Wet Groove

NASA Astrophysics Data System (ADS)

Parry, A. O.; Malijevský, A.; Rascón, C.

2014-10-01

We consider the phase equilibria of a fluid confined in a deep capillary groove of width L with identical side walls and a bottom made of a different material. All walls are completely wet by the liquid. Using density functional theory and interfacial models, we show that the meniscus separating liquid and gas phases at two phase capillary coexistence meets the bottom capped end of the groove at a capillary contact angle θcap(L) which depends on the difference between the Hamaker constants. If the bottom wall has a weaker wall-fluid attraction than the side walls, then θcap>0 even though all the isolated walls are themselves completely wet. This alters the capillary condensation transition which is now first order; this would be continuous in a capped capillary made wholly of either type of material. We show that the capillary contact angle θcap(L) vanishes in two limits, corresponding to different capillary wetting transitions. These occur as the width (i) becomes macroscopically large, and (ii) is reduced to a microscopic value determined by the difference in Hamaker constants. This second wetting transition is characterized by large scale fluctuations and essential critical singularities arising from marginal interfacial interactions.

Capillary Flow of Liquid Metals in Brazing

NASA Astrophysics Data System (ADS)

Dehsara, Mohammad

Capillary flow is driven or controlled by capillary forces, exerted at the triple line where the fluid phases meet the solid boundary. Phase field (PF) models naturally accommodate diffusive triple line motion with variable contact angle, thus allowing for the no-slip boundary condition without the stress singularities. Moreover, they are uniquely suited for modeling of topological discontinuities which often arise during capillary flows. In this study, we consider diffusive triple line motion within two PF models: the compositionally compressible (CC) and the incompressible (IC) models. We derive the IC model as a systematic approximation to the CC model, based on a suitable choice of continuum velocity field. The CC model, applied to the fluids of dissimilar mass densities, exhibits a computational instability at the triple line. The IC model perfectly represents the analytic equilibria. We develop the parameter identification procedure and show that the triple line kinetics can be well represented by the IC model's diffusive boundary condition. The IC model is first tested by benchmarking the phase-field and experimental kinetics of water, and silicone oil spreading over the glass plates in which two systems do not interact with the substrate. Then, two high-temperature physical settings involving spreading of the molten Al-Si alloy: one over a rough wetting substrate, the other over a non-wetting substrate are modeled in a T-joint structure which is a typical geometric configuration for many brazing and soldering applications. Surface roughness directly influences the spreading of the molten metal by causing break-ups of the liquid film and trapping the liquid away from the joint. In the early stages of capillary flow over non-wetting surface, the melting and flow are concurrent, so that the kinetics of wetting is strongly affected by the variations in effective viscosity of the partially molten metal. We define adequate time-dependent functions for the variations of Al-Si alloy viscosity and triple line mobility to describe the wetting kinetics.

Numerical simulation of oxygen delivery to muscle tissue in the presence of hemoglobin-based oxygen carriers.

PubMed

Patton, Jaqunda N; Palmer, Andre F

2006-01-01

This work represents a culmination of research on oxygen transport to muscle tissue, which takes into account oxygen transport due to convection, diffusion, and the kinetics of simultaneous reactions between oxygen and hemoglobin and myoglobin. The effect of adding hemoglobin-based oxygen carriers (HBOCs) to the plasma layer of blood in a single capillary surrounded by muscle tissue based on the geometry of the Krogh tissue cylinder is examined for a range of HBOC oxygen affinity, HBOC concentration, capillary inlet oxygen tension (pO(2)), and hematocrit. The full capillary length of the hamster retractor muscle was modeled under resting (V(max) = 1.57 x 10(-4) mLO(2) mL(-1) s(-1), cell velocity (v(c)) = 0.015 cm/s) and working (V(max) = 1.57 x 10(-3) mLO(2) mL(-1) s(-1), v(c) = 0.075 cm/s) conditions. Two spacings between the red blood cell (RBC) and the capillary wall were examined, corresponding to a capillary with and without an endothelial surface layer. Simulations led to the following conclusions, which lend physiological insight into oxygen transport to muscle tissue in the presence of HBOCs: (1) The reaction kinetics between oxygen and myoglobin in the tissue region, oxygen and HBOCs in the plasma, and oxygen and RBCs in the capillary lumen should not be neglected. (2) Simulation results yielded new insight into possible mechanisms of oxygen transport in the presence of HBOCs. (3) HBOCs may act as a source or sink for oxygen in the capillary and may compete with RBCs for oxygen. (4) HBOCs return oxygen delivery to muscle tissue to normal for varying degrees of hypoxia (inlet capillary pO(2) < 30 mmHg) and anemia (hematocrit < 46%) for the hamster model.

Defect reduction of high-density full-field patterns in jet and flash imprint lithography

NASA Astrophysics Data System (ADS)

Singh, Lovejeet; Luo, Kang; Ye, Zhengmao; Xu, Frank; Haase, Gaddi; Curran, David; LaBrake, Dwayne; Resnick, Douglas; Sreenivasan, S. V.

2011-04-01

Imprint lithography has been shown to be an effective technique for replication of nano-scale features. Jet and Flash Imprint Lithography (J-FIL) involves the field-by-field deposition and exposure of a low viscosity resist deposited by jetting technology onto the substrate. The patterned mask is lowered into the fluid which then quickly flows into the relief patterns in the mask by capillary action. Following this filling step, the resist is crosslinked under UV radiation, and then the mask is removed leaving a patterned resist on the substrate. Acceptance of imprint lithography for manufacturing will require demonstration that it can attain defect levels commensurate with the defect specifications of high end memory devices. Typical defectivity targets are on the order of 0.10/cm2. This work summarizes the results of defect inspections focusing on two key defect types; random non-fill defects occurring during the resist filling process and repeater defects caused by interactions with particles on the substrate. Non-fill defectivity must always be considered within the context of process throughput. The key limiting throughput step in an imprint process is resist filling time. As a result, it is critical to characterize the filling process by measuring non-fill defectivity as a function of fill time. Repeater defects typically have two main sources; mask defects and particle related defects. Previous studies have indicated that soft particles tend to cause non-repeating defects. Hard particles, on the other hand, can cause either resist plugging or mask damage. In this work, an Imprio 500 twenty wafer per hour (wph) development tool was used to study both defect types. By carefully controlling the volume of inkjetted resist, optimizing the drop pattern and controlling the resist fluid front during spreading, fill times of 1.5 seconds were achieved with non-fill defect levels of approximately 1.2/cm2. Longevity runs were used to study repeater defects and a nickel contamination was identified as the key source of particle induced repeater defects.

Theoretical research of helium pulsating heat pipe under steady state conditions

NASA Astrophysics Data System (ADS)

Xu, D.; Liu, H. M.; Li, L. F.; Huang, R. J.; Wang, W.

2015-12-01

As a new-type heat pipe, pulsating heat pipe (PHP) has several outstanding features, such as great heat transport ability, strong adjustability, small size and simple construction. PHP is a complex two-phase flow system associated with many physical subjects and parameters, which utilizes the pressure and temperature changes in volume expansion and contraction during phase changes to excite the pulsation motion of liquid plugs and vapor bubbles in the capillary tube between the evaporator and the condenser. At present time, some experimental investigation of helium PHP have been done. However, theoretical research of helium PHP is rare. In this paper, the physical and mathematical models of operating mechanism for helium PHP under steady state are established based on the conservation of mass, momentum, and energy. Several important parameters are correlated and solved, including the liquid filling ratio, flow velocity, heat power, temperature, etc. Based on the results, the operational driving force and flow resistances of helium PHP are analysed, and the flow and heat transfer is further studied.

Alignment-enhancing feed-through conductors for stackable silicon-on-sapphire wafers

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1983-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Tunable multimode-interference bandpass fiber filter.

PubMed

Antonio-Lopez, J E; Castillo-Guzman, A; May-Arrioja, D A; Selvas-Aguilar, R; Likamwa, P

2010-02-01

We report on a wavelength-tunable filter based on multimode interference (MMI) effects. A typical MMI filter consists of a multimode fiber (MMF) spliced between two single-mode fibers (SMF). The peak wavelength response of the filter exhibits a linear dependence when the length of the MMF is modified. Therefore a capillary tube filled with refractive-index-matching liquid is used to effectively increase the length of the MMF, and thus wavelength tuning is achieved. Using this filter a ring-based tunable erbium-doped fiber laser is demonstrated with a tunability of 30 nm, covering the full C-band.

Development of 3D out-of-plane SU-8 microlenses using modified micromolding in capillaries (MIMIC) technology

NASA Astrophysics Data System (ADS)

Llobera, A.; Wilke, R.; Johnson, D. W.; Büttgenbach, S.

2006-04-01

This paper describes a modification of the standard MIMIC technology, solving its main drawbacks, to define arrays of spherical or ellipsoidal microlenses. Perfectly symmetrical meniscuses have been obtained by using a XP SU-8 NO-2 layer beneath the PDMS mold. Moreover, the photostructurable properties of this polymer allow obtaining self-alignment structures for adequate fiber optics positioning. Microchannels ended with these meniscuses have been filled with standard SU-8 to obtain 3D microlenses. Agreement between theory and experimental results allows confirming the validity of the proposed technology.

Analysis of electrolyte transport through charged nanopores.

PubMed

Peters, P B; van Roij, R; Bazant, M Z; Biesheuvel, P M

2016-05-01

We revisit the classical problem of flow of electrolyte solutions through charged capillary nanopores or nanotubes as described by the capillary pore model (also called "space charge" theory). This theory assumes very long and thin pores and uses a one-dimensional flux-force formalism which relates fluxes (electrical current, salt flux, and fluid velocity) and driving forces (difference in electric potential, salt concentration, and pressure). We analyze the general case with overlapping electric double layers in the pore and a nonzero axial salt concentration gradient. The 3×3 matrix relating these quantities exhibits Onsager symmetry and we report a significant new simplification for the diagonal element relating axial salt flux to the gradient in chemical potential. We prove that Onsager symmetry is preserved under changes of variables, which we illustrate by transformation to a different flux-force matrix given by Gross and Osterle [J. Chem. Phys. 49, 228 (1968)JCPSA60021-960610.1063/1.1669814]. The capillary pore model is well suited to describe the nonlinear response of charged membranes or nanofluidic devices for electrokinetic energy conversion and water desalination, as long as the transverse ion profiles remain in local quasiequilibrium. As an example, we evaluate electrical power production from a salt concentration difference by reverse electrodialysis, using an efficiency versus power diagram. We show that since the capillary pore model allows for axial gradients in salt concentration, partial loops in current, salt flux, or fluid flow can develop in the pore. Predictions for macroscopic transport properties using a reduced model, where the potential and concentration are assumed to be invariant with radial coordinate ("uniform potential" or "fine capillary pore" model), are close to results of the full model.

A many-body dissipative particle dynamics study of forced water-oil displacement in capillary.

PubMed

Chen, Chen; Zhuang, Lin; Li, Xuefeng; Dong, Jinfeng; Lu, Juntao

2012-01-17

The forced water-oil displacement in capillary is a model that has important applications such as the groundwater remediation and the oil recovery. Whereas it is difficult for experimental studies to observe the displacement process in a capillary at nanoscale, the computational simulation is a unique approach in this regard. In the present work, the many-body dissipative particle dynamics (MDPD) method is employed to simulate the process of water-oil displacement in capillary with external force applied by a piston. As the property of all interfaces involved in this system can be manipulated independently, the dynamic displacement process is studied systematically under various conditions of distinct wettability of water in capillary and miscibility between water and oil as well as of different external forces. By analyzing the dependence of the starting force on the properties of water/capillary and water/oil interfaces, we find that there exist two different modes of the water-oil displacement. In the case of stronger water-oil interaction, the water particles cannot displace those oil particles sticking to the capillary wall, leaving a low oil recovery efficiency. To minimize the residual oil content in capillary, enhancing the wettability of water and reducing the external force will be beneficial. This simulation study provides microscopic insights into the water-oil displacement process in capillary and guiding information for relevant applications. © 2011 American Chemical Society

Toward direct pore-scale modeling of three-phase displacements

NASA Astrophysics Data System (ADS)

Mohammadmoradi, Peyman; Kantzas, Apostolos

2017-12-01

A stable spreading film between water and gas can extract a significant amount of bypassed non-aqueous phase liquid (NAPL) through immiscible three-phase gas/water injection cycles. In this study, the pore-scale displacement mechanisms by which NAPL is mobilized are incorporated into a three-dimensional pore morphology-based model under water-wet and capillary equilibrium conditions. The approach is pixel-based and the sequence of invasions is determined by the fluids' connectivity and the threshold capillary pressure of the advancing interfaces. In addition to the determination of three-phase spatial saturation profiles, residuals, and capillary pressure curves, dynamic finite element simulations are utilized to predict the effective permeabilities of the rock microtomographic images as reasonable representations of the geological formations under study. All the influential features during immiscible fluid flow in pore-level domains including wetting and spreading films, saturation hysteresis, capillary trapping, connectivity, and interface development strategies are taken into account. The capabilities of the model are demonstrated by the successful prediction of saturation functions for Berea sandstone and the accurate reconstruction of three-phase fluid occupancies through a micromodel.

Two-phase computed tomography study of warthin tumor of parotid gland: differentiation from other parotid gland tumors and its pathologic explanation.

PubMed

Woo, Seung Hoon; Choi, Dae-Seob; Kim, Jin-pyeong; Park, Jung Je; Joo, Yeon Hee; Chung, Phil-Sang; Kim, Bo-Young; Ko, Young-Hyeh; Jeong, Han-Sin; Kim, Hyung-Jin

2013-01-01

The objective of this study was to define the radiological characteristics of 2-phase computed tomography (CT) of parotid gland Warthin tumors (WTs) with a pathologic basis for these findings. We prospectively enrolled 116 patients with parotid gland tumor who underwent preoperative 2-phase CT scans(scanning delays of 30 and 120 seconds). The attenuation changes and enhancement patterns were analyzed according to pathology. We also evaluated size-matched samples of WTs and pleomorphic adenoma by staining CD31, vascular endothelial growth factor-receptor 2, collagen IV, and smooth muscle actin. Computed tomography numbers in WTs were significantly higher than those in other tumors in early-phase scans and lower in delayed scans. Pathologically, CD31(+) blood vessel area was significantly higher in WTs than in pleomorphic adenomas. In addition, WTs had an extensive capillary network and many leaky blood vessels. The enhancement pattern of early fill-in and early washout is the typical finding of WTs on 2-phase CT scans, which may be attributed pathologically to abundant blood vessel and extensive capillary network.

Ultrastructural changes in lung tissue after acute lead intoxication in the rat.

PubMed

Kaczynska, Katarzyna; Walski, Michał; Szereda-Przestaszewska, Małgorzata

2011-01-01

Pulmonary toxicity of lead was studied in rats after an intraperitoneal administration of lead acetate at a dose of 25 mg/kg. Three consecutive days of treatment increased lead content in the whole blood to 2.1 µg/dl and in lung homogenate it attained 9.62 µg/g w.w. versus control values of 0.17 µg/dl and 0.78 µg/g w.w., respectively. At the ultrastructural level, the effects of lead toxicity were observed in lung capillaries, interstitium, epithelial cells and alveolar lining layer. Accumulation of aggregated platelets, leucocytic elements and monocytes was found within capillaries. Interstitium comprised a substantial number of collagen, elastin filaments and lipofibroblasts. Lamellar bodies of type II pneumocytes contained phospolipid lamellae, which stratified into an irregular arrangement. Pulmonary alveoli were filled with macrophages. The extracellular lining layer of lung alveoli was partially destroyed. This study provided evidence that acute lead intoxication affects the whole lung parenchyma and by impairing production of the surfactant might disturb the regular respiratory function.

Development of an on-column enrichment technique based on C18-functionalized magnetic silica nanoparticles for the determination of lidocaine in rat plasma by high performance liquid chromatography.

PubMed

Chu, Bin; Lou, Dujuan; Yu, Panfeng; Hu, Shaonan; Shen, Shun

2011-10-14

In this study, a novel on-column enrichment technique filled with C(18)-functionalized magnetic silica nanoparticles was successfully developed for the determination of lidocaine in rat plasma by high performance liquid chromatography (HPLC). The synthesized Fe(3)O(4)@SiO(2)-C(18) nanoparticles were locally packed into the capillary by the application of magnets. Lidocaine in the sample solutions pumped into the capillary tube could be easily adsorbed by Fe(3)O(4)@SiO(2)-C(18) through hydrophobic interaction by the interior C(18) groups, and eluted by acetonitrile solution. Different extraction conditions were investigated. Method validations including linear range, quantification limit, detection limit, precision, accuracy and recovery were also studied. The results showed that the proposed method based on on-column enrichment by Fe(3)O(4)@SiO(2)-C(18) was a novel, little solvent and efficient approach for the determination of lidocaine in the complex plasma samples. Copyright © 2011 Elsevier B.V. All rights reserved.

Sequence requirements of oligonucleotide chiral selectors for the capillary electrophoresis resolution of low-affinity DNA binders.

PubMed

Tohala, Luma; Oukacine, Farid; Ravelet, Corinne; Peyrin, Eric

2017-05-01

We recently reported that a great variety of DNA oligonucleotides (ONs) used as chiral selectors in partial-filling capillary electrophoresis (CE) exhibited interesting enantioresolution properties toward low-affinity DNA binders. Herein, the sequence prerequisites of ONs for the CE enantioseparation process were studied. First, the chiral resolution properties of a series of homopolymeric sequences (Poly-dT) of different lengths (from 5 to 60-mer) were investigated. It was shown that the size increase-dependent random coil-like conformation of Poly-dT favorably acted on the apparent selectivity and resolution. The base-unpairing state constituted also an important factor in the chiral resolution ability of ONs as the switch from the single-stranded to double-stranded structure was responsible for a significant decrease in the analyte selectivity range. Finally, the chemical diversity enhanced the enantioresolution ability of single-stranded ONs. The present work could lay the foundation for the design of performant ON chiral selectors for the CE separation of weak DNA binder enantiomers. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Influence of tissue metabolism and capillary oxygen supply on arteriolar oxygen transport: a computational model.

PubMed

Moschandreou, T E; Ellis, C G; Goldman, D

2011-07-01

We present a theoretical model for steady-state radial and longitudinal oxygen transport in arterioles containing flowing blood (plasma and red blood cells) and surrounded by living tissue. This model combines a detailed description of convective and diffusive oxygen transport inside the arteriole with a novel boundary condition at the arteriolar lumen surface, and the results provide new mass transfer coefficients for computing arteriolar O(2) losses based on far-field tissue O(2) tension and in the presence of spatially distributed capillaries. A numerical procedure is introduced for calculating O(2) diffusion from an arteriole to a continuous capillary-tissue matrix immediately adjacent to the arteriole. The tissue O(2) consumption rate is assumed to be constant and capillaries act as either O(2) sources or sinks depending on the local O(2) environment. Using the model, O(2) saturation (SO(2)) and tension (PO(2)) are determined for the intraluminal region of the arteriole, as well as for the extraluminal region in the neighbouring tissue. Our model gives results that are consistent with available experimental data and previous intraluminal transport models, including appreciable radial decreases in intraluminal PO(2) for all vessel diameters considered (12-100 μm) and slower longitudinal decreases in PO(2) for larger vessels than for smaller ones, and predicts substantially less diffusion of O(2) from arteriolar blood than do models with PO(2) specified at the edge of the lumen. The dependence of the new mass transfer coefficients on vessel diameter, SO(2) and far-field PO(2) is calculated allowing their application to a wide range of physiological situations. This novel arteriolar O(2) transport model will be a vital component of future integrated models of microvascular regulation of O(2) supply to capillary beds and the tissue regions they support. Copyright © 2011 Elsevier Inc. All rights reserved.

The effect of contact angles and capillary dimensions on the burst frequency of super hydrophilic and hydrophilic centrifugal microfluidic platforms, a CFD study.

PubMed

Kazemzadeh, Amin; Ganesan, Poo; Ibrahim, Fatimah; He, Shuisheng; Madou, Marc J

2013-01-01

This paper employs the volume of fluid (VOF) method to numerically investigate the effect of the width, height, and contact angles on burst frequencies of super hydrophilic and hydrophilic capillary valves in centrifugal microfluidic systems. Existing experimental results in the literature have been used to validate the implementation of the numerical method. The performance of capillary valves in the rectangular and the circular microfluidic structures on super hydrophilic centrifugal microfluidic platforms is studied. The numerical results are also compared with the existing theoretical models and the differences are discussed. Our experimental and computed results show a minimum burst frequency occurring at square capillaries and this result is useful for designing and developing more sophisticated networks of capillary valves. It also predicts that in super hydrophilic microfluidics, the fluid leaks consistently from the capillary valve at low pressures which can disrupt the biomedical procedures in centrifugal microfluidic platforms.

The Effect of Contact Angles and Capillary Dimensions on the Burst Frequency of Super Hydrophilic and Hydrophilic Centrifugal Microfluidic Platforms, a CFD Study

PubMed Central

Kazemzadeh, Amin; Ganesan, Poo; Ibrahim, Fatimah; He, Shuisheng; Madou, Marc J.

2013-01-01

This paper employs the volume of fluid (VOF) method to numerically investigate the effect of the width, height, and contact angles on burst frequencies of super hydrophilic and hydrophilic capillary valves in centrifugal microfluidic systems. Existing experimental results in the literature have been used to validate the implementation of the numerical method. The performance of capillary valves in the rectangular and the circular microfluidic structures on super hydrophilic centrifugal microfluidic platforms is studied. The numerical results are also compared with the existing theoretical models and the differences are discussed. Our experimental and computed results show a minimum burst frequency occurring at square capillaries and this result is useful for designing and developing more sophisticated networks of capillary valves. It also predicts that in super hydrophilic microfluidics, the fluid leaks consistently from the capillary valve at low pressures which can disrupt the biomedical procedures in centrifugal microfluidic platforms. PMID:24069169

Van der waals forces on thin liquid films in capillary tubes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Herdt, G.C.; Swanson, L.W.

1993-10-01

A theory of the van der Waals attraction between a thin liquid films and a capillary tube is presented assuming the presence of a vapor-liquid interface. The model is based on the surface mode analysis method of van Kampen et al. Values for the van der Waals interaction energy per unit area were calculated for liquid films of pentane on a gold substrate assuming a thin liquid film. Results indicate that the effect of capillary curvature on the van der Waals interaction increases as the ratio of the liquid film thickness to the capillary radius is increased. This trend ismore » consistent with predictions based on the Hamaker theory. Deviations from results based on the Hamaker theory are easily explained in terms of retardation of the van der Waals interaction. Because the effect of capillary curvature increases in the regime where retardation effects become important, curvature effects constitute a small correction to the van der Waals forces in a capillary tube.« less

Mach-like capillary-gravity wakes.

PubMed

Moisy, Frédéric; Rabaud, Marc

2014-08-01

We determine experimentally the angle α of maximum wave amplitude in the far-field wake behind a vertical surface-piercing cylinder translated at constant velocity U for Bond numbers Bo(D)=D/λ(c) ranging between 0.1 and 4.2, where D is the cylinder diameter and λ(c) the capillary length. In all cases the wake angle is found to follow a Mach-like law at large velocity, α∼U(-1), but with different prefactors depending on the value of Bo(D). For small Bo(D) (large capillary effects), the wake angle approximately follows the law α≃c(g,min)/U, where c(g,min) is the minimum group velocity of capillary-gravity waves. For larger Bo(D) (weak capillary effects), we recover a law α∼√[gD]/U similar to that found for ship wakes at large velocity [Rabaud and Moisy, Phys. Rev. Lett. 110, 214503 (2013)]. Using the general property of dispersive waves that the characteristic wavelength of the wave packet emitted by a disturbance is of order of the disturbance size, we propose a simple model that describes the transition between these two Mach-like regimes as the Bond number is varied. We show that the new capillary law α≃c(g,min)/U originates from the presence of a capillary cusp angle (distinct from the usual gravity cusp angle), along which the energy radiated by the disturbance accumulates for Bond numbers of order of unity. This model, complemented by numerical simulations of the surface elevation induced by a moving Gaussian pressure disturbance, is in qualitative agreement with experimental measurements.

Theoretical model for optical oximetry at the capillary level: exploring hemoglobin oxygen saturation through backscattering of single red blood cells

NASA Astrophysics Data System (ADS)

Liu, Rongrong; Spicer, Graham; Chen, Siyu; Zhang, Hao F.; Yi, Ji; Backman, Vadim

2017-02-01

Oxygen saturation (sO2) of red blood cells (RBCs) in capillaries can indirectly assess local tissue oxygenation and metabolic function. For example, the altered retinal oxygenation in diabetic retinopathy and local hypoxia during tumor development in cancer are reflected by abnormal sO2 of local capillary networks. However, it is far from clear whether accurate label-free optical oximetry (i.e., measuring hemoglobin sO2) is feasible from dispersed RBCs at the single capillary level. The sO2-dependent hemoglobin absorption contrast present in optical scattering signal is complicated by geometry-dependent scattering from RBCs. We present a numerical study of backscattering spectra from single RBCs based on the first-order Born approximation, considering practical factors: RBC orientations, size variation, and deformations. We show that the oscillatory spectral behavior of RBC geometries is smoothed by variations in cell size and orientation, resulting in clear sO2-dependent spectral contrast. In addition, this spectral contrast persists with different mean cellular hemoglobin content and different deformations of RBCs. This study shows for the first time the feasibility of, and provides a theoretical model for, label-free optical oximetry at the single capillary level using backscattering-based imaging modalities, challenging the popular view that such measurements are impossible at the single capillary level.

Stereological Study on the Positive Effect of Running Exercise on the Capillaries in the Hippocampus in a Depression Model.

PubMed

Chen, Linmu; Zhou, Chunni; Tan, Chuanxue; Wang, Feifei; Gao, Yuan; Huang, Chunxia; Zhang, Yi; Jiang, Lin; Tang, Yong

2017-01-01

Running exercise is an effective method to improve depressive symptoms when combined with drugs. However, the underlying mechanisms are not fully clear. Cerebral blood flow perfusion in depressed patients is significantly lower in the hippocampus. Physical activity can achieve cerebrovascular benefits. The purpose of this study was to evaluate the impacts of running exercise on capillaries in the hippocampal CA1 and dentate gyrus (DG) regions. The chronic unpredictable stress (CUS) depression model was used in this study. CUS rats were given 4 weeks of running exercise from the fifth week to the eighth week (20 min every day from Monday to Friday each week). The sucrose consumption test was used to measure anhedonia. Furthermore, stereological methods were used to investigate the capillary changes among the control group, CUS/Standard group and CUS/Running group. Sucrose consumption significantly increased in the CUS/Running group. Running exercise has positive effects on the capillaries parameters in the hippocampal CA1 and DG regions, such as the total volume, total length and total surface area. These results demonstrated that capillaries are protected by running exercise in the hippocampal CA1 and DG might be one of the structural bases for the exercise-induced treatment of depression-like behavior. These results suggest that drugs and behavior influence capillaries and may be considered as a new means for depression treatment in the future.

Running exercise protects the capillaries in white matter in a rat model of depression.

PubMed

Chen, Lin-Mu; Zhang, Ai-Pin; Wang, Fei-Fei; Tan, Chuan-Xue; Gao, Yuan; Huang, Chun-Xia; Zhang, Yi; Jiang, Lin; Zhou, Chun-Ni; Chao, Feng-Lei; Zhang, Lei; Tang, Yong

2016-12-01

Running has been shown to improve depressive symptoms when used as an adjunct to medication. However, the mechanisms underlying the antidepressant effects of running are not fully understood. Changes of capillaries in white matter have been discovered in clinical patients and depression model rats. Considering the important part of white matter in depression, running may cause capillary structural changes in white matter. Chronic unpredictable stress (CUS) rats were provided with a 4-week running exercise (from the fifth week to the eighth week) for 20 minutes each day for 5 consecutive days each week. Anhedonia was measured by a behavior test. Furthermore, capillary changes were investigated in the control group, the CUS/Standard group, and the CUS/Running group using stereological methods. The 4-week running increased sucrose consumption significantly in the CUS/Running group and had significant effects on the total volume, total length, and total surface area of the capillaries in the white matter of depression rats. These results demonstrated that exercise-induced protection of the capillaries in white matter might be one of the structural bases for the exercise-induced treatment of depression. It might provide important parameters for further study of the vascular mechanisms of depression and a new research direction for the development of clinical antidepressant means. J. Comp. Neurol. 524:3577-3586, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Incorporating the Impacts of Small Scale Rock Heterogeneity into Models of Flow and Trapping in Target UK CO2 Storage Systems

NASA Astrophysics Data System (ADS)

Jackson, S. J.; Reynolds, C.; Krevor, S. C.

2017-12-01

Predictions of the flow behaviour and storage capacity of CO2 in subsurface reservoirs are dependent on accurate modelling of multiphase flow and trapping. A number of studies have shown that small scale rock heterogeneities have a significant impact on CO2flow propagating to larger scales. The need to simulate flow in heterogeneous reservoir systems has led to the development of numerical upscaling techniques which are widely used in industry. Less well understood, however, is the best approach for incorporating laboratory characterisations of small scale heterogeneities into models. At small scales, heterogeneity in the capillary pressure characteristic function becomes significant. We present a digital rock workflow that combines core flood experiments with numerical simulations to characterise sub-core scale capillary pressure heterogeneities within rock cores from several target UK storage reservoirs - the Bunter, Captain and Ormskirk sandstone formations. Measured intrinsic properties (permeability, capillary pressure, relative permeability) and 3D saturations maps from steady-state core flood experiments were the primary inputs to construct a 3D digital rock model in CMG IMEX. We used vertical end-point scaling to iteratively update the voxel by voxel capillary pressure curves from the average MICP curve; with each iteration more closely predicting the experimental saturations and pressure drops. Once characterised, the digital rock cores were used to predict equivalent flow functions, such as relative permeability and residual trapping, across the range of flow conditions estimated to prevail in the CO2 storage reservoirs. In the case of the Captain sandstone, rock cores were characterised across an entire 100m vertical transect of the reservoir. This allowed analysis of the upscaled impact of small scale heterogeneity on flow and trapping. Figure 1 shows the varying degree to which heterogeneity impacted flow depending on the capillary number in the Captain sandstone. At low capillary numbers, typical of regions where flow is dominated by buoyancy, fluid flow is impeded and trapping enhanced. At high capillary numbers, typical of the near wellbore environment, the fluid distributed homogeneously and the equivalent relative permeability was higher leading to improved injectivity.

Modeling CO2 Storage in Fractured Reservoirs: Fracture-Matrix Interactions of Free-Phase and Dissolved CO2

NASA Astrophysics Data System (ADS)

Oldenburg, C. M.; Zhou, Q.; Birkholzer, J. T.

2017-12-01

The injection of supercritical CO2 (scCO2) in fractured reservoirs has been conducted at several storage sites. However, no site-specific dual-continuum modeling for fractured reservoirs has been reported and modeling studies have generally underestimated the fracture-matrix interactions. We developed a conceptual model for enhanced CO2 storage to take into account global scCO2 migration in the fracture continuum, local storage of scCO2 and dissolved CO2 (dsCO2) in the matrix continuum, and driving forces for scCO2 invasion and dsCO2 diffusion from fractures. High-resolution discrete fracture-matrix models were developed for a column of idealized matrix blocks bounded by vertical and horizontal fractures and for a km-scale fractured reservoir. The column-scale simulation results show that equilibrium storage efficiency strongly depends on matrix entry capillary pressure and matrix-matrix connectivity while the time scale to reach equilibrium is sensitive to fracture spacing and matrix flow properties. The reservoir-scale modeling results shows that the preferential migration of scCO2 through fractures is coupled with bulk storage in the rock matrix that in turn retards the fracture scCO2 plume. We also developed unified-form diffusive flux equations to account for dsCO2 storage in brine-filled matrix blocks and found solubility trapping is significant in fractured reservoirs with low-permeability matrix.

Computational modeling of the structure-function relationship in human placental terminal villi.

PubMed

Plitman Mayo, R; Olsthoorn, J; Charnock-Jones, D S; Burton, G J; Oyen, M L

2016-12-08

Placental oxygen transport takes place at the final branches of the villous tree and is dictated by the relative arrangement of the maternal and fetal circulations. Modeling techniques have failed to accurately assess the structure-function relationship in the terminal villi due to the geometrical complexity. Three-dimensional blood flow and oxygen transport was modeled in four terminal villi reconstructed from confocal image stacks. The blood flow was analyzed along the center lines of capillary segments and the effect of the variability in capillary diameter, tortuosity and branching was investigated. Additionally, a validation study was performed to corroborate the simulation results. The results show how capillary variations impact motion of the fetal blood, and how their bends and dilatations can decelerate the flow by up to 80%. Vortical flow is also demonstrated not to develop in the fetal capillaries. The different geometries are shown to dictate the transport of gases with differences of over 100% in the oxygen flux between samples. Capillary variations are key for efficient oxygen uptake by the fetus; they allow the blood to decelerate where the villous membrane is thinnest allowing for a better oxygenation, but also by reducing the vessel diameter they carry the oxygenated blood away fast. The methodology employed herein could become a platform to simulate complicated in-vivo and in-vitro scenarios of pregnancy complications. Copyright © 2016 Elsevier Ltd. All rights reserved.

The relative influence of hematocrit and red blood cell velocity on oxygen transport from capillaries to tissue.

PubMed

Lücker, Adrien; Secomb, Timothy W; Weber, Bruno; Jenny, Patrick

2017-04-01

Oxygen transport to parenchymal cells occurs mainly at the microvascular level and depends on convective RBC flux, which is proportional in an individual capillary to the product of capillary hematocrit and RBC velocity. This study investigates the relative influence of these two factors on tissue PO 2 . A simple analytical model is used to quantify the respective influences of hematocrit, RBC velocity, and RBC flow on tissue oxygenation around capillaries. Predicted tissue PO 2 levels are compared with a detailed computational model. Hematocrit is shown to have a larger influence on tissue PO 2 than RBC velocity. The effect of RBC velocity increases with distance from the arterioles. Good agreement between analytical and numerical results is obtained, and the discrepancies are explained. Significant dependence of MTCs on RBC velocity at low hematocrit is demonstrated. For a given RBC flux in a capillary, the PO 2 in the surrounding tissue increases with increasing hematocrit, as a consequence of decreasing IVR to diffusive oxygen transport from RBCs to tissue. These results contribute to understanding the effects of blood flow changes on oxygen transport, such as those that occur in functional hyperemia in the brain. © 2016 John Wiley & Sons Ltd.

3D printed Lego®-like modular microfluidic devices based on capillary driving.

PubMed

Nie, Jing; Gao, Qing; Qiu, Jing-Jiang; Sun, Miao; Liu, An; Shao, Lei; Fu, Jian-Zhong; Zhao, Peng; He, Yong

2018-03-12

The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however, extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. The key rationale for this method is to print different functional modules with a low-cost three-dimensional (3D) printer, then fill the channels with capillary materials and assemble them with plugs like Lego ® bricks. This rapidly reconstructed modular microfluidic device consists of a variety of common functional modules and other personalized modules, each module having a unified standard interface for easy assembly. As it can be printed by a desktop 3D printer, the manufacturing process is simple and efficient, with controllable regulation of the flow channel scale. Through diverse combinations of different modules, a variety of different functions can be achieved, without duplicating the manufacturing process. A single module can also be taken out for testing and analysis. What's more, combined with basic circuit components, it can serve as a low-cost Lego ® -like modular microfluidic circuits. As a proof of concept, the modular microfluidic device has been successfully demonstrated and used for stent degradation and cell cultures, revealing the potential use of this method in both chemical and biological research.

Automatic detection of recoil-proton tracks and background rejection criteria in liquid scintillator-micro-capillary-array fast neutron spectrometer

NASA Astrophysics Data System (ADS)

Mor, Ilan; Vartsky, David; Dangendorf, Volker; Tittelmeier, Kai.; Weierganz, Mathias; Goldberg, Mark Benjamin; Bar, Doron; Brandis, Michal

2018-06-01

We describe an analysis procedure for automatic unambiguous detection of fast-neutron-induced recoil proton tracks in a micro-capillary array filled with organic liquid scintillator. The detector is viewed by an intensified CCD camera. This imaging neutron detector possesses the capability to perform high position-resolution (few tens of μm), energy-dispersive transmission-imaging using ns-pulsed beams. However, when operated with CW or DC beams, it also features medium-quality spectroscopic capabilities for incident neutrons in the energy range 2-20 MeV. In addition to the recoil proton events which display a continuous extended track structure, the raw images exhibit complex ion-tracks from nuclear interactions of fast-neutrons in the scintillator, capillaries quartz-matrix and CCD. Moreover, as expected, one also observes a multitude of isolated scintillation spots of varying intensity (henceforth denoted "blobs") that originate from several different sources, such as: fragmented proton tracks, gamma-rays, heavy-ion reactions as well as events and noise that occur in the image-intensifier and CCD. In order to identify the continuous-track recoil proton events and distinguish them from all these background events, a rapid, computerized and automatic track-recognition-procedure was developed. Based on an appropriately weighted analysis of track parameters such as: length, width, area and overall light intensity, the method is capable of distinguishing a single continuous-track recoil proton from typically surrounding several thousands of background events that are found in each CCD frame.

Modeling oxygen transport in human placental terminal villi.

PubMed

Gill, J S; Salafia, C M; Grebenkov, D; Vvedensky, D D

2011-12-21

Oxygen transport from maternal blood to fetal blood is a primary function of the placenta. Quantifying the effectiveness of this exchange remains key in identifying healthy placentas because of the great variability in capillary number, caliber and position within the villus-even in placentas deemed clinically "normal". By considering villous membrane to capillary membrane transport, stationary oxygen diffusion can be numerically solved in terminal villi represented by digital photomicrographs. We aim to provide a method to determine whether and if so to what extent diffusional screening may operate in placental villi. Segmented digital photomicrographs of terminal villi from the Pregnancy, Infection and Nutrition study in North Carolina 2002 are used as a geometric basis for solving the stationary diffusion equation. Constant maternal villous oxygen concentration and perfect fetal capillary membrane absorption are assumed. System efficiency is defined as the ratio of oxygen flux into a villus and the sum of the capillary areas contained within. Diffusion screening is quantified by comparing numerical and theoretical maximum oxygen fluxes. A strong link between various measures of villous oxygen transport efficiency and the number of capillaries within a villus is established. The strength of diffusional screening is also related to the number of capillaries within a villus. Our measures of diffusional efficiency are shown to decrease as a function of the number of capillaries per villus. This low efficiency, high capillary number relationship supports our hypothesis that diffusional screening is present in this system. Oxygen transport per capillary is reduced when multiple capillaries compete for diffusing oxygen. A complete picture of oxygen fluxes, capillary and villus areas is obtainable and presents an opportunity for future work. Copyright © 2011 Elsevier Ltd. All rights reserved.

A comparison of capillary hydraulic conductivities in postural and locomotor muscle.

PubMed

McDonagh, P F; Gore, R W

1982-09-01

In a comparative skeletal muscle study Folkow and Halicka (Microvasc. Res. 1: 1-14, 1968) reported that the capillary filtration coefficient (CFC) of postural (red) muscle was two times the CFC of locomotor (white) muscle. It was concluded that the twofold difference in CFC was due solely to a difference in the perfused capillary surface areas (Sf) of red vs. white muscle. However, CFC is the product of capillary hydraulic conductivity (LP) and Sf. Hence their conclusion assumed that the average LP of red muscle capillaries is exactly equal to the average LP of white muscle capillaries. The following study was undertaken to test the validity of this assumption. The microocclusion procedures and analytical model described by Lee et al. (Circ. Res. 28: 358-370, 1971) and Gore [Am. J. Physiol. 242 (Heart Circ. Physiol. 11): H268-H287, 1982] were used to determine LP. Independent measurements of LP were recorded from single capillaries in red, anterior latissimus dorsi (ALD) and white, posterior latissimus dorsi (PLD) muscles of chickens anesthetized with L.A. Thesia. We found that the mean capillary hydraulic conductivity in postural muscle [(LP)ALD = 0.20 +/- 0.06 (SE) micrometers . s-1 . cmH2O-1 (n = 11)] was significantly different from the mean capillary hydraulic conductivity in locomotor muscle [(LP)PLD = 0.061 +/- 0.01 micrometers . s-1 . cmH2O-1 (n = 14)] (P less than 0.05). These results provide direct evidence that observed differences in red vs. white muscle CFC's may not be due solely to different perfused capillary surface areas but may also be due to differences in capillary hydraulic conductivity.

Multiscale modeling of fluid transport in tumors.

PubMed

Chapman, S Jonathan; Shipley, Rebecca J; Jawad, Rossa

2008-11-01

A model for fluid flow through the leaky neovasculature and porous interstitium of a solid tumor is developed. A network of isolated capillaries is analyzed in the limit of small capillary radius, and analytical expressions for the hydraulic conductivities and fractional leakage coefficients derived. This model is then homogenized to give a continuum description in terms of the vascular density. The resulting equations comprise a double porous medium with coupled Darcy flow through the interstitium and vasculature.

Heat Transfer by Thermo-capillary Convection -Sounding Rocket COMPERE Experiment SOURCE

NASA Astrophysics Data System (ADS)

Dreyer, Michael; Fuhrmann, Eckart

The sounding rocket COMPERE experiment SOURCE was successfully flown on MASER 11, launched in Kiruna (ESRANGE), May 15th, 2008. SOURCE has been intended to partly ful-fill the scientific objectives of the European Space Agency (ESA) Microgravity Applications Program (MAP) project AO-2004-111 (Convective boiling and condensation). Three parties of principle investigators have been involved to design the experiment set-up: ZARM for thermo-capillary flows, IMFT (Toulouse, France) for boiling studies, EADS Astrium (Bremen, Ger-many) for depressurization. The topic of this paper is to study the effect of wall heat flux on the contact line of the free liquid surface and to obtain a correlation for a convective heat trans-fer coefficient. The experiment has been conducted along a predefined time line. A preheating sequence at ground was the first operation to achieve a well defined temperature evolution within the test cell and its environment inside the rocket. Nearly one minute after launch, the pressurized test cell was filled with the test liquid HFE-7000 until a certain fill level was reached. Then the free surface could be observed for 120 s without distortion. Afterwards, the first depressurization was started to induce subcooled boiling, the second one to start saturated boiling. The data from the flight consists of video images and temperature measurements in the liquid, the solid, and the gaseous phase. Data analysis provides the surface shape versus time and the corresponding apparent contact angle. Computational analysis provides information for the determination of the heat transfer coefficient in a compensated gravity environment where a flow is caused by the temperature difference between the hot wall and the cold liquid. The paper will deliver correlations for the effective contact angle and the heat transfer coefficient as a function of the relevant dimensionsless parameters as well as physical explanations for the observed behavior. The data will be used for benchmarking of commercial CFD codes and the tank design (using these codes) of future cryogenic upper stages. References Eckart Fuhrmann, Michael E. Dreyer, Description of the Sounding Rocket Experiment SOURCE, Microgravity sci. technol., 20/3-4, 206 (2008)

High throughput nanoimprint lithography for semiconductor memory applications

NASA Astrophysics Data System (ADS)

Ye, Zhengmao; Zhang, Wei; Khusnatdinov, Niyaz; Stachowiak, Tim; Irving, J. W.; Longsine, Whitney; Traub, Matthew; Fletcher, Brian; Liu, Weijun

2017-03-01

Imprint lithography is a promising technology for replication of nano-scale features. For semiconductor device applications, Canon deposits a low viscosity resist on a field by field basis using jetting technology. A patterned mask is lowered into the resist fluid which then quickly flows into the relief patterns in the mask by capillary action. Following this filling step, the resist is crosslinked under UV radiation, and then the mask is removed, leaving a patterned resist on the substrate. There are two critical components to meeting throughput requirements for imprint lithography. Using a similar approach to what is already done for many deposition and etch processes, imprint stations can be clustered to enhance throughput. The FPA-1200NZ2C is a four station cluster system designed for high volume manufacturing. For a single station, throughput includes overhead, resist dispense, resist fill time (or spread time), exposure and separation. Resist exposure time and mask/wafer separation are well understood processing steps with typical durations on the order of 0.10 to 0.20 seconds. To achieve a total process throughput of 17 wafers per hour (wph) for a single station, it is necessary to complete the fluid fill step in 1.2 seconds. For a throughput of 20 wph, fill time must be reduced to only one 1.1 seconds. There are several parameters that can impact resist filling. Key parameters include resist drop volume (smaller is better), system controls (which address drop spreading after jetting), Design for Imprint or DFI (to accelerate drop spreading) and material engineering (to promote wetting between the resist and underlying adhesion layer). In addition, it is mandatory to maintain fast filling, even for edge field imprinting. In this paper, we address the improvements made in all of these parameters to first enable a 1.20 second filling process for a device like pattern and have demonstrated this capability for both full fields and edge fields. Non-fill defectivity is well under 1.0 defects/cm2 for both field types. Next, by further reducing drop volume and optimizing drop patterns, a fill time of 1.1 seconds was demonstrated.

Modification of narrow ablating capillaries under the influence of multiple femtosecond laser pulses

NASA Astrophysics Data System (ADS)

Gubin, K. V.; Lotov, K. V.; Trunov, V. I.; Pestryakov, E. V.

2016-09-01

Powerful femtosecond laser pulses that propagate through narrow ablating capillaries cause modification of capillary walls, which is studied experimentally and theoretically. At low intensities, the laser-induced periodic surface structures and porous coating composed of sub-micron particles appear on the walls. At higher intensities, the surface is covered by deposited droplets of the size up to 10 μm. In both cases, the ablated material forms a solid plug that completely blocks the capillary after several hundreds or thousands of pulses. The suggested theoretical model indicates that the plug formation is a universal effect. It must take place in any narrow tube subject to ablation under the action of short laser pulses.

Phenotypic heterogeneity in lung capillary and extra-alveolar endothelial cells. Increased extra-alveolar endothelial permeability is sufficient to decrease compliance.

PubMed

Lowe, Kevin; Alvarez, Diego; King, Judy; Stevens, Troy

2007-11-01

In acute respiratory distress syndrome, pulmonary vascular permeability increases, causing intravascular fluid and protein to move into the lung's interstitium. The classic model describing the formation of pulmonary edema suggests that fluid crossing the capillary endothelium is drawn by negative interstitial pressure into the potential space surrounding extra-alveolar vessels and, as interstitial pressure builds, is forced into the alveolar air space. However, the validity of this model is challenged by animal models of acute lung injury in which extra-alveolar vessels are more permeable than capillaries under a variety of conditions. In the current study, we sought to determine whether extravascular fluid accumulation can be produced because of increased permeability of either the capillary or extra-alveolar endothelium, and whether different pathophysiology results from such site-specific increases in permeability. We perfused isolated lungs with either the plant alkaloid thapsigargin, which increases extra-alveolar endothelial permeability, or with 4alpha-phorbol 12, 13-didecanoate, which increases capillary endothelial permeability. Both treatments produced equal increases in whole lung vascular permeability, but caused fluid accumulations in separate anatomical compartments. Light microscopy of isolated lungs showed that thapsigargin caused fluid cuffing of large vessels, while 4alpha-phorbol 12, 13-didecanoate caused alveolar flooding. Dynamic compliance was reduced in lungs with cuffing of large vessels, but not in lungs with alveolar flooding. Phenotypic differences between vascular segments resulted in site-specific increases in permeability, which have different pathophysiological outcomes. Our findings suggest that insults leading to acute respiratory distress syndrome may increase permeability in extra-alveolar or capillary vascular segments, resulting in different pathophysiological sequela.

In vivo flow speed measurement of capillaries by photoacoustic correlation spectroscopy.

PubMed

Chen, Sung-Liang; Xie, Zhixing; Carson, Paul L; Wang, Xueding; Guo, L Jay

2011-10-15

We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and high sensitivity, enabling sufficient signals from single red blood cells. The probe beam size is calibrated by a blood-mimicking phantom. The results indicate the feasibility of using PACS to study flow speeds in capillaries.

Design and Operation of a Cryogenic Nitrogen Pulsating Heat Pipe

NASA Astrophysics Data System (ADS)

Diego Fonseca, Luis; Miller, Franklin; Pfotenhauer, John

2015-12-01

We report the design, experimental setup and successful test results using an innovative passive cooling system called a “Pulsating Heat Pipe” (PHP) operating at temperatures ranging from 77 K to 80 K and using nitrogen as the working fluid. PHPs, which transfer heat by two phase flow mechanisms through a closed loop tubing have the advantage that no electrical pumps are needed to drive the fluid flow. In addition, PHPs have an advantage over copper straps and thermal conductors since they are lighter in weight, exhibit lower temperature gradients and have higher heat transfer rates. PHPs consist of an evaporator section, thermally anchored to a solid, where heat is received at the saturation temperature where the liquid portion of the two-phase flow evaporates, and a condenser where heat is rejected at the saturation temperature where the vapor is condensed. The condenser section in our experiment has been thermally interfaced to a CT cryocooler from SunPower that has a cooling capacity of 10 W at 77 K. Alternating regions of liquid slugs and small vapor plugs fill the capillary tubing, with the vapor regions contracting in the condenser section and expanding in the evaporator section due to an electric heater that will generate heat loads up to 10 W. This volumetric expansion and contraction provides the oscillatory flow of the fluid throughout the capillary tubing thereby transferring heat from one end to the other. The thermal performance and temperature characteristics of the PHP will be correlated as a function of average condenser temperature, PHP fill liquid ratio, and evaporator heat load. The experimental data show that the heat transfer between the evaporator and condenser sections can produce an effective thermal conductivity up to 35000 W/m-K at a 3.5 W heat load.

Drop deformation and breakup in a partially filled horizontal rotating cylinder

NASA Astrophysics Data System (ADS)

White, Andrew; Pereira, Caroline; Hyacinthe, Hyaquino; Ward, Thomas

2014-11-01

Drop deformation and breakup due to shear flow has been studied extensively in Couette devices as well as in gravity-driven flows. In these cases shear is generated either by the moving wall or the drop's motion. For such flows the drop shape remains unperturbed at low capillary number (Ca), deforms at moderate Ca , and can experience breakup as Ca --> 1 and larger. Here single drops of NaOH(aq) will be placed in a horizontal cylindrical rotating tank partially filled with vegetable oil resulting in 10-2 < Ca <101 . It will be shown that the reactive vegetable oil-NaOH(aq) system, where surfactants are produced in situ by saponification, can yield lower minimum surface tensions and faster adsorption than non-reactive surfactant systems. Oil films between the wall and drop as well as drop shape will be observed as rotation rates and NaOH(aq) concentration are varied. Results will be presented in the context of previous work on bubble and drop shapes and breakup. NSF CBET #1262718.

Experimental analysis for fabrication of high-aspect-ratio piezoelectric ceramic structure by micro-powder injection molding process

NASA Astrophysics Data System (ADS)

Han, Jun Sae; Gal, Chang Woo; Park, Jae Man; Kim, Jong Hyun; Park, Seong Jin

2018-04-01

Aspect ratio effects in the micro-powder injection molding process were experimentally analyzed for fabrication of high-aspect-ratio piezoelectric ceramic structure. The mechanisms of critical defects have been studied according to individual manufacturing steps. In the molding process, incomplete filling phenomenon determines the critical aspect ratios of a micro pattern. According to mold temperature, an incomplete filling phenomenon has been analyzed with respect to different pattern sizes and aspect ratio. In demolding and drying process, the capillary behavior of sacrificial polymeric mold insert determines the critical aspect ratio of a micro pattern. With respect to pattern dimensions, slumping behavior has been analyzed. Based on our current systems, micro PZT feature has stability when it has lower aspect ratio than 5. Under optimized processing conditions, 20 μm and 40 μm ceramic rod array feature which has 5 of aspect ratio were successfully fabricated by the developed process. Further modification points to fabricate the smaller and higher feature were specifically addressed.

A two-angle model of dynamic wetting in microscale capillaries under low capillary numbers with experiments.

PubMed

Lei, Da; Lin, Mian; Li, Yun; Jiang, Wenbin

2018-06-15

An accurate model of the dynamic contact angle θ d is critical for the calculation of capillary force in applications like enhanced oil recovery, where the capillary number Ca ranges from 10 -10 to 10 -5 and the Bond number Bo is less than 10 -4 . The rate-dependence of the dynamic contact angle under such conditions remains blurred, and is the main target of this study. Featuring with pressure control and interface tracking, the innovative experimental system presented in this work achieves the desired ranges of Ca and Bo, and enables the direct optical measurement of dynamic contact angles in capillaries as tiny as 40 × 20 (width × height) μm and 80 × 20 μm. The advancing and receding processes of wetting and nonwetting liquids were tested. The dynamic contact angle was confirmed velocity-independent with 10 -9  < Ca < 10 -5 (contact line velocity V = 0.135-490 μm/s) and it can be described by a two-angle model with desirable accuracy. A modified two-angle model was developed and an empirical form was obtained from experiments. For different liquids contacting the same surface, the advancing angle θ adv approximately equals the static contact angle θ o . The receding angle θ rec was found to be a linear function of θ adv , in good agreement with our and other experiments from the literature. Copyright © 2018 Elsevier Inc. All rights reserved.

Interior Temperature Measurement Using Curved Mercury Capillary Sensor Based on X-ray Radiography

NASA Astrophysics Data System (ADS)

Chen, Shuyue; Jiang, Xing; Lu, Guirong

2017-07-01

A method was presented for measuring the interior temperature of objects using a curved mercury capillary sensor based on X-ray radiography. The sensor is composed of a mercury bubble, a capillary and a fixed support. X-ray digital radiography was employed to capture image of the mercury column in the capillary, and a temperature control system was designed for the sensor calibration. We adopted livewire algorithms and mathematical morphology to calculate the mercury length. A measurement model relating mercury length to temperature was established, and the measurement uncertainty associated with the mercury column length and the linear model fitted by least-square method were analyzed. To verify the system, the interior temperature measurement of an autoclave, which is totally closed, was taken from 29.53°C to 67.34°C. The experiment results show that the response of the system is approximately linear with an uncertainty of maximum 0.79°C. This technique provides a new approach to measure interior temperature of objects.

Gas Flow and Ion Transfer in Heated ESI Capillary Interfaces

NASA Astrophysics Data System (ADS)

Bernier, Laurent; Pinfold, Harry; Pauly, Matthias; Rauschenbach, Stephan; Reiss, Julius

2018-02-01

Transfer capillaries are the preferred means to transport ions, generated by electrospray ionization, from ambient conditions to vacuum. During the transfer of ions through the narrow, long tubes into vacuum, substantial losses are typical. However, recently it was demonstrated that these losses can be avoided altogether. To understand the experimental observation and provide a general model for the ion transport, here, we investigate the ion transport through capillaries by numerical simulation of interacting ions. The simulation encompasses all relevant factors, such as space charge, diffusion, gas flow, and heating. Special attention is paid to the influence of the gas flow on the transmission and especially the change imposed by heating. The gas flow is modeled by a one-dimensional gas dynamics description. A large number of ions are treated as point particles in this gas flow. This allows to investigate the influence of the capillary heating on the gas flow and by this on the ion transport. The results are compared with experimental findings. [Figure not available: see fulltext.

Capillary-induced crack healing between surfaces of nanoscale roughness.

PubMed

Soylemez, Emrecan; de Boer, Maarten P

2014-10-07

Capillary forces are important in nature (granular materials, insect locomotion) and in technology (disk drives, adhesion). Although well studied in equilibrium state, the dynamics of capillary formation merit further investigation. Here, we show that microcantilever crack healing experiments are a viable experimental technique for investigating the influence of capillary nucleation on crack healing between rough surfaces. The average crack healing velocity, v̅, between clean hydrophilic polycrystalline silicon surfaces of nanoscale roughness is measured. A plot of v̅ versus energy release rate, G, reveals log-linear behavior, while the slope |d[log(v̅)]/dG| decreases with increasing relative humidity. A simplified interface model that accounts for the nucleation time of water bridges by an activated process is developed to gain insight into the crack healing trends. This methodology enables us to gain insight into capillary bridge dynamics, with a goal of attaining a predictive capability for this important microelectromechanical systems (MEMS) reliability failure mechanism.

Modelling of capillary-driven flow for closed paper-based microfluidic channels

NASA Astrophysics Data System (ADS)

Songok, Joel; Toivakka, Martti

2017-06-01

Paper-based microfluidics is an emerging field focused on creating inexpensive devices, with simple fabrication methods for applications in various fields including healthcare, environmental monitoring and veterinary medicine. Understanding the flow of liquid is important in achieving consistent operation of the devices. This paper proposes capillary models to predict flow in paper-based microfluidic channels, which include a flow accelerating hydrophobic top cover. The models, which consider both non-absorbing and absorbing substrates, are in good agreement with the experimental results.

In vitro models for evaluation of periodontal wound healing/regeneration.

PubMed

Weinreb, Miron; Nemcovsky, Carlos E

2015-06-01

Periodontal wound healing and regeneration are highly complex processes, involving cells, matrices, molecules and genes that must be properly choreographed and orchestrated. As we attempt to understand and influence these clinical entities, we need experimental models to mimic the various aspects of human wound healing and regeneration. In vivo animal models that simulate clinical situations of humans can be costly and cumbersome. In vitro models have been devised to dissect wound healing/regeneration processes into discrete, analyzable steps. For soft tissue (e.g. gingival) healing, in vitro models range from simple culture of cells grown in monolayers and exposed to biological modulators or physical effectors and materials, to models in which cells are 'injured' by scraping and subsequently the 'wound' is filled with new or migrating cells, to three-dimensional models of epithelial-mesenchymal recombination or tissue explants. The cells employed are gingival keratinocytes, fibroblasts or endothelial cells, and their proliferation, migration, attachment, differentiation, survival, gene expression, matrix production or capillary formation are measured. Studies of periodontal regeneration also include periodontal ligament fibroblasts or progenitors, osteoblasts or osteoprogenitors, and cementoblasts. Regeneration models measure cellular proliferation, attachment and migration, as well as gene expression, transfer and differentiation into a mineralizing phenotype and biomineralization. Only by integrating data from models on all levels (i.e. a single cell to the whole organism) can various critical aspects of periodontal wound healing/regeneration be fully evaluated. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Effect of buoyancy on the motion of long bubbles in horizontal tubes

NASA Astrophysics Data System (ADS)

Atasi, Omer; Khodaparast, Sepideh; Scheid, Benoit; Stone, Howard A.

2017-09-01

As a confined long bubble translates along a horizontal liquid-filled tube, a thin film of liquid is formed on the tube wall. For negligible inertial and buoyancy effects, respectively, small Reynolds (Re) and Bond (Bo) numbers, the thickness of the liquid film depends only on the flow capillary number (Ca). However, buoyancy effects are no longer negligible as the diameter of the tube reaches millimeter length scales, which corresponds to finite values of Bo. We perform experiments and theoretical analysis for a long bubble in a horizontal tube to investigate the effect of Bond number (0.05

A computational model of oxygen transport in the cerebrocapillary levels for normal and pathologic brain function.

PubMed

Safaeian, Navid; David, Tim

2013-10-01

The oxygen exchange and correlation between the cerebral blood flow (CBF) and cerebral metabolic rate of oxygen consumption (CMRO2) in the cortical capillary levels for normal and pathologic brain functions remain the subject of debate. A 3D realistic mesoscale model of the cortical capillary network (non-tree like) is constructed using a random Voronoi tessellation in which each edge represents a capillary segment. The hemodynamics and oxygen transport are numerically simulated in the model, which involves rheological laws in the capillaries, oxygen diffusion, and non-linear binding of oxygen to hemoglobin, respectively. The findings show that the cerebral hypoxia due to a significant decreased perfusion (as can occur in stroke) can be avoided by a moderate reduction in oxygen demand. Oxygen extraction fraction (OEF) can be an important indicator for the brain oxygen metabolism under normal perfusion and misery-perfusion syndrome (leading to ischemia). The results demonstrated that a disproportionately large increase in blood supply is required for a small increase in the oxygen demand, which, in turn, is strongly dependent on the resting OEF. The predicted flow-metabolism coupling in the model supports the experimental studies of spatiotemporal stimulations in humans by positron emission tomography and functional magnetic resonance imaging.

A computational model of oxygen transport in the cerebrocapillary levels for normal and pathologic brain function

PubMed Central

Safaeian, Navid; David, Tim

2013-01-01

The oxygen exchange and correlation between the cerebral blood flow (CBF) and cerebral metabolic rate of oxygen consumption (CMRO2) in the cortical capillary levels for normal and pathologic brain functions remain the subject of debate. A 3D realistic mesoscale model of the cortical capillary network (non-tree like) is constructed using a random Voronoi tessellation in which each edge represents a capillary segment. The hemodynamics and oxygen transport are numerically simulated in the model, which involves rheological laws in the capillaries, oxygen diffusion, and non-linear binding of oxygen to hemoglobin, respectively. The findings show that the cerebral hypoxia due to a significant decreased perfusion (as can occur in stroke) can be avoided by a moderate reduction in oxygen demand. Oxygen extraction fraction (OEF) can be an important indicator for the brain oxygen metabolism under normal perfusion and misery-perfusion syndrome (leading to ischemia). The results demonstrated that a disproportionately large increase in blood supply is required for a small increase in the oxygen demand, which, in turn, is strongly dependent on the resting OEF. The predicted flow-metabolism coupling in the model supports the experimental studies of spatiotemporal stimulations in humans by positron emission tomography and functional magnetic resonance imaging. PMID:23921901

A simple expression for quantifying bacterial chemotaxis using capillary assay data: application to the analysis of enhanced chemotactic responses from growth-limited cultures.

PubMed

Ford, R M; Lauffenburger, D A

1992-05-01

An individual cell-based mathematical model of Rivero et al. provides a framework for determining values of the chemotactic sensitivity coefficient chi 0, an intrinsic cell population parameter that characterizes the chemotactic response of bacterial populations. This coefficient can theoretically relate the swimming behavior of individual cells to the resulting migration of a bacterial population. When this model is applied to the commonly used capillary assay, an approximate solution can be obtained for a particular range of chemotactic strengths yielding a very simple analytical expression for estimating the value of chi 0, [formula: see text] from measurements of cell accumulation in the capillary, N, when attractant uptake is negligible. A0 and A infinity are the dimensionless attractant concentrations initially present at the mouth of the capillary and far into the capillary, respectively, which are scaled by Kd, the effective dissociation constant for receptor-attractant binding. D is the attractant diffusivity, and mu is the cell random motility coefficient. NRM is the cell accumulation in the capillary in the absence of an attractant gradient, from which mu can be determined independently as mu = (pi/4t)(NRM/pi r2bc)2, with r the capillary tube radius and bc the bacterial density initially in the chamber. When attractant uptake is significant, a slightly more involved procedure requiring a simple numerical integration becomes necessary. As an example, we apply this approach to quantitatively characterize, in terms of the chemotactic sensitivity coefficient chi 0, data from Terracciano indicating enhanced chemotactic responses of Escherichia coli to galactose when cultured under growth-limiting galactose levels in a chemostat.

A Concurrent Flow Model for Extraction during Transcapillary Passage

PubMed Central

Bassingthwaighte, James B.

2010-01-01

A model for capillary-tissue exchange in a uniformly perfused organ with uniform capillary transit times and no diffusional capillary interactions was designed to permit the exploration of the influences of various parameters on the interpretation of indicator-dilution curves obtained at the venous outflow following the simultaneous injection of tracers into the arterial inflow. These parameters include tissue geometric factors, longitudinal diffusion and volumes of distribution of tracers in blood and tissue, hematocrit, volumes of nonexchanging vessels and the sampling system, capillary permeability, P. capillary surface area, S, and flow of blood- or solute-containing fluid, Fs′. An assumption of instantaneous radial diffusion in the extravascular region is appropriate when intercapillary distances are small, as they are in the heart, or permeabilities are low, as they are for lipophobic solutes. Numerical solutions were obtained for dispersed input functions similar to normal intravascular dye-dilution curves. Axial extravascular diffusion showed a negligible influence at low permeabilities. The “instantaneous extraction” of a permeating solute can provide an estimate of PS/Fs′, the ratio of the capillary permeability–surface area product to the flow, when PS/Fs′ lies between approximately 0.05 and 3.0; the limits of the range depend on the extravascular volume of distribution and the influences of intravascular dispersion. The most accurate estimates were obtained when experiments were designed so that PS/Fs′ was between 0.2 and 1.0 or peak extractions were between 0.1 and 0.6. PMID:4608628

Verification of an analytic modeler for capillary pump loop thermal control systems

NASA Technical Reports Server (NTRS)

Schweickart, R. B.; Neiswanger, L.; Ku, J.

1987-01-01

A number of computer programs have been written to model two-phase heat transfer systems for space use. These programs support the design of thermal control systems and provide a method of predicting their performance in the wide range of thermal environments of space. Predicting the performance of one such system known as the capillary pump loop (CPL) is the intent of the CPL Modeler. By modeling two developed CPL systems and comparing the results with actual test data, the CPL Modeler has proven useful in simulating CPL operation. Results of the modeling effort are discussed, together with plans for refinements to the modeler.

CO2 Capillary-Trapping Processes in Deep Saline Aquifers

NASA Astrophysics Data System (ADS)

Gershenzon, Naum I.; Soltanian, Mohamadreza; Ritzi, Robert W., Jr.; Dominic, David F.

2014-05-01

The idea of reducing the Earth's greenhouse effect by sequestration of CO2 into the Earth's crust has been discussed and evaluated for more than two decades. Deep saline aquifers are the primary candidate formations for realization of this idea. Evaluation of reservoir capacity and the risk of CO2 leakage require a detailed modeling of the migration and distribution of CO2 in the subsurface structure. There is a finite risk that structural (or hydrodynamic) trapping by caprock may be compromised (e.g. by improperly abandoned wells, stratigraphic discontinuities, faults, etc.). Therefore, other trapping mechanisms (capillary trapping, dissolution, and mineralization) must be considered. Capillary trapping may be very important in providing a "secondary-seal", and is the focus of our investigation. The physical mechanism of CO2 trapping in porous media by capillary trapping incorporates three related processes, i.e. residual trapping, trapping due to hysteresis of the relative permeability, and trapping due to hysteresis of the capillary pressure. Additionally CO2 may be trapped in heterogeneous media due to difference in capillary pressure entry points for different materials. The amount of CO2 trapped by these processes is a complicated nonlinear function of the spatial distribution of permeability, permeability anisotropy, capillary pressure, relative permeability of brine and CO2, permeability hysteresis and residual gas saturation (as well as the rate, total amount and placement of injected CO2). Geological heterogeneities essentially affect the dynamics of a CO2 plume in subsurface environments. Recent studies have led to new conceptual and quantitative models for sedimentary architecture in fluvial deposits over a range of scales that are relevant to the performance of some deep saline reservoirs [1, 2]. We investigated how the dynamics of a CO2 plume, during and after injection, is influenced by the hierarchical and multi-scale stratal architecture in such reservoirs. The results strongly suggest that representing these small scales features, and representing how they are organized within a hierarchy of larger-scale features, is critical to understanding capillary trapping processes. References [1] Bridge, J.S. (2006), Fluvial facies models: Recent developments, in Facies Models Revisited, SEPM Spec. Publ., 84, edited by H. W. Posamentier and R. G. Walker, pp. 85-170, Soc. for Sediment. Geol. (SEPM), Tulsa, Okla [2] Ramanathan, R., A. Guin, R.W. Ritzi, D.F. Dominic, V.L. Freedman, T.D. Scheibe, and I.A. Lunt (2010), Simulating the heterogeneity in channel belt deposits: Part 1. A geometric-based methodology and code, Water Resources Research, v. 46, W04515.

Failure of Ibuprofen to prevent progressive dermal ischemia after burning in guinea pigs.

PubMed

Tan, Qian; Lin, Zihao; Ma, Wenxi; Chen, Huairen; Wang, Lei; Ning, Guansen; Zhou, Xu

2002-08-01

It is controversial whether the use of prostaglandin inhibitors could prevent progressive dermal ischemia in the postburn stasis zone. This study evaluated the effect of Ibuprofen on preventing postburn dermal ischemia using an animal model of India ink perfusion and skin transparent preparation techniques. The closely clipped backs of the guinea pigs were bathed in 75 degrees C water for 10s. Ibuprofen-treated groups were fed intragastrically with Ibuprofen (12.5mg/kg) every 6h. All animals were perfused with 70% India ink via a cervical artery cannula at 16 kPa constant pressure at 0, 8, 16, 24h postburn. Skin transparent preparations were made, and 6-keto-PGF(1 alpha) and T x B(2) levels in skin tissue were assessed. India ink filling rates in skin capillary plexuses decreased gradually with postburn time elapsing (P<0.01). 6-keto-PGF(1 alpha) and T x B(2) levels in two groups increased. The increase of T x B(2) was dominant, which was related to postburn dermal ischemia (r=0.742, P<0.01). Though levels of 6-keto-PGF(1 alpha) and T x B(2) decreased in Ibuprofen-treated groups, India ink filling rates showed no significant difference between controls and experimental groups (P>0.05). The results were also confirmed by observation of skin transparent preparations. This study suggests that Ibuprofen has no preventive effect on progressive dermal ischemia after burning.

Numerical investigations of two-phase flow with dynamic capillary pressure in porous media via a moving mesh method

NASA Astrophysics Data System (ADS)

Zhang, Hong; Zegeling, Paul Andries

2017-09-01

Motivated by observations of saturation overshoot, this paper investigates numerical modeling of two-phase flow in porous media incorporating dynamic capillary pressure. The effects of the dynamic capillary coefficient, the infiltrating flux rate and the initial and boundary values are systematically studied using a traveling wave ansatz and efficient numerical methods. The traveling wave solutions may exhibit monotonic, non-monotonic or plateau-shaped behavior. Special attention is paid to the non-monotonic profiles. The traveling wave results are confirmed by numerically solving the partial differential equation using an accurate adaptive moving mesh solver. Comparisons between the computed solutions using the Brooks-Corey model and the laboratory measurements of saturation overshoot verify the effectiveness of our approach.

Analysis of flow dynamics through small diameter gas sampling systems

NASA Technical Reports Server (NTRS)

Brown, K. G.

1984-01-01

The removal of gas material through a capillary opening in a surface is analyzed. The gas, from which the sample is removed, is moving past the surface at supersonic velocities. A variety of possible conditions of temperature, pressure and composition are discussed in an effort to emulate conditions that might be found at the surface of a vehicle traversing the altitude range 100-50 km, or might exist at the surface of a model in the stream of a high enthalpy wind tunnel. Aspects discussed include: (1) the throughput of the capillary for conditions of different lengths and different L/a (length/radius) ratios; (2) the total throughput when the surface in question contains many hundreds of these capillaries; (3) the effect of the capillaries upon the composition of the analyzed gas; (4) the effect of the capillary or capillaries upon the gas stream itself; and (5) the implications of the calculations upon the possible implementation of this type of device as an inlet for a mass spectrometer to be developed for analyzing the upper atmosphere.

Capillary pressure heterogeneity and hysteresis for the supercritical CO2/water system in a sandstone

NASA Astrophysics Data System (ADS)

Pini, Ronny; Benson, Sally M.

2017-10-01

We report results from an experimental investigation on the hysteretic behaviour of the capillary pressure curve for the supercritical CO2-water system in a Berea Sandstone core. Previous observations have highlighted the importance of subcore-scale capillary heterogeneity in developing local saturations during drainage; we show in this study that the same is true for the imbibition process. Spatially distributed drainage and imbibition scanning curves were obtained for mm-scale subsets of the rock sample non-invasively using X-ray CT imagery. Core- and subcore-scale measurements are well described using the Brooks-Corey formalism, which uses a linear trapping model to compute mobile saturations during imbibition. Capillary scaling yields two separate universal drainage and imbibition curves that are representative of the full subcore-scale data set. This enables accurate parameterisation of rock properties at the subcore-scale in terms of capillary scaling factors and permeability, which in turn serve as effective indicators of heterogeneity at the same scale even when hysteresis is a factor. As such, the proposed core-analysis workflow is quite general and provides the required information to populate numerical models that can be used to extend core-flooding experiments to conditions prevalent in the subsurface, which would be otherwise not attainable in the laboratory.

The relative influence of hematocrit and red blood cell velocity on oxygen transport from capillaries to tissue

PubMed Central

Lücker, Adrien; Secomb, Timothy W.; Weber, Bruno; Jenny, Patrick

2016-01-01

Objective Oxygen transport to parenchymal cells occurs mainly at the microvascular level, and depends on convective red blood cell (RBC) flux, which is proportional in an individual capillary to the product of capillary hematocrit and red blood cell velocity. This study investigates the relative influence of these two factors on tissue oxygen partial pressure (Po2). Methods A simple analytical model is used to quantify the respective influences of hematocrit, RBC velocity and flow on tissue oxygenation around capillaries. Predicted tissue Po2 levels are compared with a detailed computational model. Results Hematocrit is shown to have a larger influence on tissue Po2 than RBC velocity. The effect of RBC velocity increases with distance from the arterioles. Good agreement between analytical and numerical results is obtained and the discrepancies are explained. Significant dependence of mass transfer coefficients on RBC velocity at low hematocrit is demonstrated. Conclusions For a given RBC flux in a capillary, the Po2 in the surrounding tissue increases with increasing hematocrit, as a consequence of decreasing intravascular resistance to diffusive oxygen transport from RBCs to tissue. These results contribute to understanding the effects of blood flow changes on oxygen transport, such as occur in functional hyperemia in the brain. PMID:27893186

Method for making alignment-enhancing feed-through conductors for stackable silicon-on-sapphire

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1985-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process of this invention involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Experimental characterization of active plasma lensing for electron beams

NASA Astrophysics Data System (ADS)

Pompili, R.; Anania, M. P.; Bellaveglia, M.; Biagioni, A.; Bini, S.; Bisesto, F.; Brentegani, E.; Castorina, G.; Chiadroni, E.; Cianchi, A.; Croia, M.; Di Giovenale, D.; Ferrario, M.; Filippi, F.; Giribono, A.; Lollo, V.; Marocchino, A.; Marongiu, M.; Mostacci, A.; Di Pirro, G.; Romeo, S.; Rossi, A. R.; Scifo, J.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

2017-03-01

The active plasma lens represents a compact and affordable tool with radially symmetric focusing and field gradients up to several kT/m. In order to be used as a focusing device, its effects on the particle beam distribution must be well characterized. Here, we present the experimental results obtained by focusing an high-brightness electron beam by means of a 3 cm-long discharge-capillary pre-filled with Hydrogen gas. We achieved minimum spot sizes of 24 μ m (rms) showing that, during plasma lensing, the beam emittance increases due to nonlinearities in the focusing field. The results have been cross-checked with numerical simulations, showing an excellent agreement.

Fast-acting self-healing metallic fuse.

NASA Technical Reports Server (NTRS)

Schwartz, F. C.; Renton, C. A.; Rabinovici, B.

1971-01-01

Description of a fast-acting nonmechanical self-healing mercury fuse capable of protecting a high current circuit or device from overcurrent fault damages. Basically the self-healing fuse consists of two enclosed mercury reservoirs connected by a fine capillary tube filled with mercury that serves as the fusing element. It is pointed out that a better understanding of the energy conversion process involved in the operation of the device could help explore other device configurations (such as a tapering geometry and use of magnetic field to drive the arc into the fuse wall on inductive loads, etc.) and thus extend the range of capabilities for this type of protective device.

Hemoglobin-based O2 carrier O2 affinity and capillary inlet pO2 are important factors that influence O2 transport in a capillary.

PubMed

Dimino, Michael L; Palmer, Andre F

2007-01-01

Hemopure (Biopure; Cambridge, MA) and PolyHeme (Northfield Laboratories; Evanston, IL) are two acellular hemoglobin-based O2 carriers (HBOCs) currently in phase III clinical trials for use as red blood cell substitutes. The most common adverse side effect that these HBOCs exhibit is increased vasoconstriction. Autoregulatory theory has been presented as a possible explanation for this physiological effect, where it is hypothesized that low-affinity HBOCs over-deliver O2 to tissues surrounding arterioles, thereby eliciting vasoconstriction. In this paper, we wanted to investigate HBOC oxygenation of tissue surrounding a capillary, which is the smallest element of the circulatory system. An a priori model has been developed in which the performance of mixtures of acellular HBOCs (synthesized by our group and others) and human red blood cells (hRBCs) has been simulated using a Krogh tissue cylinder model (KTCM) comprising a capillary surrounded by a capillary membrane and skeletal muscle tissue in cylindrical coordinates with specified tissue O2 consumption rates and Michaelis-Menten kinetics. In this study, the total hemoglobin (hRBCs and HBOCs) concentration was kept constant. The HBOCs studied possessed O2 affinities that were higher and lower compared to hRBCs (P50's spanned 5-55 mmHg), and the equilibrium binding/release of oxygen to/from the HBOCs was modeled using the Adair equation. At normoxic inlet pO2's, there was no correlation between O2 flux out of the capillary and the O2 affinity of the HBOC. However, a correlation was found between the average pO2 tension in the capillary and the O2 affinity of the HBOC. Additionally, we studied the change in the O2 equilibrium curve of HBOCs with different O2 affinities over a wide range of inlet pO2's and found that changing the inlet pO2 greatly affected which HBOC, having a unique O2 affinity, best delivered O2 to the surrounding tissue. The analysis of oxygen transport presented could lead to a better prediction of which acellular HBOC is best suited for a specific transfusion application that many times depends on the capillary inlet pO2 tension.

The fabrication of highly ordered block copolymer micellar arrays: control of the separation distances of silicon oxide dots

NASA Astrophysics Data System (ADS)

Yoo, Hana; Park, Soojin

2010-06-01

We demonstrate the fabrication of highly ordered silicon oxide dotted arrays prepared from polydimethylsiloxane (PDMS) filled nanoporous block copolymer (BCP) films and the preparation of nanoporous, flexible Teflon or polyimide films. Polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP) films were annealed in toluene vapor to enhance the lateral order of micellar arrays and were subsequently immersed in alcohol to produce nano-sized pores, which can be used as templates for filling a thin layer of PDMS. When a thin layer of PDMS was spin-coated onto nanoporous BCP films and thermally annealed at a certain temperature, the PDMS was drawn into the pores by capillary action. PDMS filled BCP templates were exposed to oxygen plasma environments in order to fabricate silicon oxide dotted arrays. By addition of PS homopolymer to PS-b-P2VP copolymer, the separation distances of micellar arrays were tuned. As-prepared silicon oxide dotted arrays were used as a hard master for fabricating nanoporous Teflon or polyimide films by spin-coating polymer precursor solutions onto silicon patterns and peeling off. This simple process enables us to fabricate highly ordered nanoporous BCP templates, silicon oxide dots, and flexible nanoporous polymer patterns with feature size of sub-20 nm over 5 cm × 5 cm.

The fabrication of highly ordered block copolymer micellar arrays: control of the separation distances of silicon oxide dots.

PubMed

Yoo, Hana; Park, Soojin

2010-06-18

We demonstrate the fabrication of highly ordered silicon oxide dotted arrays prepared from polydimethylsiloxane (PDMS) filled nanoporous block copolymer (BCP) films and the preparation of nanoporous, flexible Teflon or polyimide films. Polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP) films were annealed in toluene vapor to enhance the lateral order of micellar arrays and were subsequently immersed in alcohol to produce nano-sized pores, which can be used as templates for filling a thin layer of PDMS. When a thin layer of PDMS was spin-coated onto nanoporous BCP films and thermally annealed at a certain temperature, the PDMS was drawn into the pores by capillary action. PDMS filled BCP templates were exposed to oxygen plasma environments in order to fabricate silicon oxide dotted arrays. By addition of PS homopolymer to PS-b-P2VP copolymer, the separation distances of micellar arrays were tuned. As-prepared silicon oxide dotted arrays were used as a hard master for fabricating nanoporous Teflon or polyimide films by spin-coating polymer precursor solutions onto silicon patterns and peeling off. This simple process enables us to fabricate highly ordered nanoporous BCP templates, silicon oxide dots, and flexible nanoporous polymer patterns with feature size of sub-20 nm over 5 cm x 5 cm.

Abnormalities of capillary microarchitecture in a rat model of coronary ischemic congestive heart failure

PubMed Central

Chen, Jiqiu; Yaniz-Galende, Elisa; Kagan, Heather J.; Liang, Lifan; Hekmaty, Saboor; Giannarelli, Chiara

2015-01-01

The aim of the present study is to explore the role of capillary disorder in coronary ischemic congestive heart failure (CHF). CHF was induced in rats by aortic banding plus ischemia-reperfusion followed by aortic debanding. Coronary arteries were perfused with plastic polymer containing fluorescent dye. Multiple fluorescent images of casted heart sections and scanning electric microscope of coronary vessels were obtained to characterize changes in the heart. Cardiac function was assessed by echocardiography and in vivo hemodynamics. Stenosis was found in all levels of the coronary arteries in CHF. Coronary vasculature volume and capillary density in remote myocardium were significantly increased in CHF compared with control. This occurred largely in microvessels with a diameter of ≤3 μm. Capillaries in CHF had a tortuous structure, while normal capillaries were linear. Capillaries in CHF had inconsistent diameters, with assortments of narrowed and bulged segments. Their surfaces appeared rough, potentially indicating endothelial dysfunction in CHF. Segments of main capillaries between bifurcations were significantly shorter in length in CHF than in control. Transiently increasing preload by injecting 50 μl of 30% NaCl demonstrated that the CHF heart had lower functional reserve; this may be associated with congestion in coronary microcirculation. Ischemic coronary vascular disorder is not limited to the main coronary arteries, as it occurs in arterioles and capillaries. Capillary disorder in CHF included stenosis, deformed structure, proliferation, and roughened surfaces. This disorder in the coronary artery architecture may contribute to the reduction in myocyte contractility in the setting of heart failure. PMID:25659485

Multianalyte detection using a capillary-based flow immunosensor.

PubMed

Narang, U; Gauger, P R; Kusterbeck, A W; Ligler, F S

1998-01-01

A highly sensitive, dual-analyte detection system using capillary-based immunosensors has been designed for explosive detection. This model system consists of two capillaries, one coated with antibodies specific for 2,4,6-trinitrotoluene (TNT) and the other specific for hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) combined into a single device. The fused silica capillaries are prepared by coating anti-TNT and anti-RDX antibodies onto the silanized inner walls using a hetero-bifunctional crosslinker. After immobilization, the antibodies are saturated with a suitable fluorophorelabeled antigen. A "T" connector is used to continuously flow the buffer solution through the individual capillaries. To perform the assay, an aliquot of TNT or RDX or a mixture of the two analytes is injected into the continuous flow stream. In each capillary, the target analyte displaces the fluorophore-labeled antigen from the binding pocket of the antibody. The labeled antigen displaced from either capillary is detected downstream using two portable spectrofluorometers. The limits of detection for TNT and RDX in the multi-analyte formate are 44 fmol (100 microliters of 0.1 ng/ml TNT solution) and 224 fmol (100 microliters of 0.5 ng/ml RDX solution), respectively. The entire assay for both analytes can be performed in less than 3 min.

Cell adhesion during bullet motion in capillaries.

PubMed

Takeishi, Naoki; Imai, Yohsuke; Ishida, Shunichi; Omori, Toshihiro; Kamm, Roger D; Ishikawa, Takuji

2016-08-01

A numerical analysis is presented of cell adhesion in capillaries whose diameter is comparable to or smaller than that of the cell. In contrast to a large number of previous efforts on leukocyte and tumor cell rolling, much is still unknown about cell motion in capillaries. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to "bullet-like" motion, with a consequent decrease in the velocity of the cell. A state diagram was obtained for various values of capillary diameter and receptor density. We found that bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis. Copyright © 2016 the American Physiological Society.

A three-dimensional neural spheroid model for capillary-like network formation.

PubMed

Boutin, Molly E; Kramer, Liana L; Livi, Liane L; Brown, Tyler; Moore, Christopher; Hoffman-Kim, Diane

2018-04-01

In vitro three-dimensional neural spheroid models have an in vivo-like cell density, and have the potential to reduce animal usage and increase experimental throughput. The aim of this study was to establish a spheroid model to study the formation of capillary-like networks in a three-dimensional environment that incorporates both neuronal and glial cell types, and does not require exogenous vasculogenic growth factors. We created self-assembled, scaffold-free cellular spheroids using primary-derived postnatal rodent cortex as a cell source. The interactions between relevant neural cell types, basement membrane proteins, and endothelial cells were characterized by immunohistochemistry. Transmission electron microscopy was used to determine if endothelial network structures had lumens. Endothelial cells within cortical spheroids assembled into capillary-like networks with lumens. Networks were surrounded by basement membrane proteins, including laminin, fibronectin and collagen IV, as well as key neurovascular cell types. Existing in vitro models of the cortical neurovascular environment study monolayers of endothelial cells, either on transwell inserts or coating cellular spheroids. These models are not well suited to study vasculogenesis, a process hallmarked by endothelial cell cord formation and subsequent lumenization. The neural spheroid is a new model to study the formation of endothelial cell capillary-like structures in vitro within a high cell density three-dimensional environment that contains both neuronal and glial populations. This model can be applied to investigate vascular assembly in healthy or disease states, such as stroke, traumatic brain injury, or neurodegenerative disorders. Copyright © 2017 Elsevier B.V. All rights reserved.

A mixture theory model of fluid and solute transport in the microvasculature of normal and malignant tissues. I. Theory.

PubMed

Schuff, M M; Gore, J P; Nauman, E A

2013-05-01

In order to better understand the mechanisms governing transport of drugs, nanoparticle-based treatments, and therapeutic biomolecules, and the role of the various physiological parameters, a number of mathematical models have previously been proposed. The limitations of the existing transport models indicate the need for a comprehensive model that includes transport in the vessel lumen, the vessel wall, and the interstitial space and considers the effects of the solute concentration on fluid flow. In this study, a general model to describe the transient distribution of fluid and multiple solutes at the microvascular level was developed using mixture theory. The model captures the experimentally observed dependence of the hydraulic permeability coefficient of the capillary wall on the concentration of solutes present in the capillary wall and the surrounding tissue. Additionally, the model demonstrates that transport phenomena across the capillary wall and in the interstitium are related to the solute concentration as well as the hydrostatic pressure. The model is used in a companion paper to examine fluid and solute transport for the simplified case of an axisymmetric geometry with no solid deformation or interconversion of mass.

A two scale modeling and computational framework for vibration-induced Raynaud syndrome.

PubMed

Hua, Yue; Lemerle, Pierre; Ganghoffer, Jean-François

2017-07-01

Hand-Arm Vibration syndrome (HAVS), usually caused by long-term use of hand-held power tools, can in certain manifestations alter the peripheral blood circulation in the hand-arm region. HAVS typically occurs after exposure to cold, causing an abnormally strong vasoconstriction of blood vessels. A pathoanatomical mechanism suggests that a reduction of the lumen of the blood vessels in VWF (Vibration White Finger) subjects, due to either hypertrophy or thickening of the vessel wall, may be at the origin of the disease. However, the direct and indirect effects of the load of the hand-held tools on the structure of blood vessels remain controversial:.one hypothesis is the mechanical action of vibration on the local acral dysregulation and/or on the vessel histomorphological modifications. Another hypothesis is the participation of the sympathetic nervous system to this dysregulation. In this paper, we assume the modifications as mechanobiological growth and the load-effect relationship may be interpreted as directly or indirectly induced. This work is the first attempt to model the effect of vibration through soft tissues onto the distal capillaries, addressing the double paradigm of multi space-time scales, i.e. low period vibration versus high time constant of the growth phenomenon as well as vibrations propagating in the macroscopic tissue including the microscopic capillary structures subjected to a pathological microstructural evolution. The objective is to lay down the theoretical basis of growth modeling for the small distal artery, with the ability to predict the geometrical and structural changes of the arterial walls caused by vibration exposure. We adopt the key idea of splitting the problem into one global vibration problem at the macroscopic scale and one local growth problem at the micro level. The macroscopic hyperelastic viscous dynamic model of the fingertip cross-section is validated by fitting experimental data. It is then used in steady-state vibration conditions to predict the mechanical fields in the close vicinity of capillaries. The space scale transfer from macroscopic to microscopic levels is ensured by considering a representative volume element (RVE) embedding a single capillary in its center. The vibrations emitted by the hand held power tool are next linked to the capillary growth through the adopted biomechanical growth model at the capillary level. The obtained results show that vibrations induce an increase of the thickness of the capillary's wall, thereby confirming the scenario of vibrations induced reduction of the lumen of blood vessels. Copyright © 2017 Elsevier Ltd. All rights reserved.

Numerical modeling of fluid and oxygen exchanges through microcirculation for the assessment of microcirculation alterations caused by type 2 diabetes.

PubMed

Tang, Yuanliang; He, Ying

2018-05-01

Type 2 diabetes mellitus (DM2) is frequently accompanied by microcirculation complications, including structural and functional alterations, which may have serious effects on substance exchanges between blood and interstitial tissue and the health of organs. In this paper, we aim to study the influence of microcirculation alterations in DM2 patients on fluid and oxygen exchanges through a model analysis. A fluid flow and oxygen transport model were developed by considering the interplay between blood in capillary network and interstitial tissue. The two regions were separately represented by 1D network model and 3D volume model, and the immersed boundary method (IBM) was adopted to solve fluid and mass transfer between these two regions. By using the model, the steady flow field and the distributions of oxygen in capillary network and surrounding tissue were firstly simulated. In the interstitial volume, fluid pressure and oxygen tension decreased with the increase of distance from the network; in the network, oxygen tension in blood plasma dropped from 100 mm Hg at the entrance to about 40 mm Hg at the exit. We further tested several structural and functional disorders related to diabetic pathological conditions. Simulated results show that the impaired connectivity of the network could result in poor robustness in maintaining blood flow and perfused surface; under high fluid permeability conditions of capillary walls, the pressure gradient was much larger around the capillary bed, and this alteration led to a saturation level of the interstitial pressure when lymphatic flow drainage can't work effectively; the variations in network connectivity and permeability of capillary wall also had unfavorable influence on oxygen distributions in interstitial tissue. In addition, when the oxygen releasing capacity of hemoglobin was confined by glycosylated hemoglobin (HbA1) in the case of diabetes, the plasma could not be complemented with adequate oxygen and thus the hypoxic tissue range will be extended. This study illustrates that when microcirculation disturbances, including the structure of capillary network, the wall osmosis property and the capacity of blood binding oxygen occur in DM2, some negative impacts are raised on microvascular hemodynamics and metabolism circumstance of interstitial tissue. Copyright © 2018 Elsevier Inc. All rights reserved.

Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification.

PubMed

Horká, Marie; Karásek, Pavel; Salplachta, Jiří; Růžička, Filip; Vykydalová, Marie; Kubesová, Anna; Dráb, Vladimír; Roth, Michal; Slais, Karel

2013-07-25

In this study, combination of capillary isoelectric focusing (CIEF) in tapered fused silica (FS) capillary with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is presented as an efficient approach for unambiguous identification of probiotic bacteria in real sample. For this purpose, bacteria within genus Lactobacillus were selected as model bioanalytes and cow's milk was selected as a biological sample. CIEF analysis of both the cultivated bacteria and the bacteria in the milk was optimized and isoelectric points characterizing the examined bacteria were subsequently determined independently of the bacterial sample origin. The use of tapered FS capillary significantly enhanced the separation capacity and efficiency of the CIEF analyses performed. In addition, the cell number injected into the tapered FS capillary was quantified and an excellent linearity of the calibration curves was achieved which enabled quantitative analysis of the bacteria by CIEF with UV detection. The minimum detectable number of bacterial cells was 2×10(6) mL(-1). Finally, cow's milk spiked with the selected bacterium was analyzed by CIEF in tapered FS capillary, the focused and detected bacterial cells were collected from the capillary, deposited onto the cultivation medium, and identified using MALDI-TOF MS afterward. Our results have revealed that the proposed procedure can be advantageously used for unambiguous identification of probiotic bacteria in a real sample. Copyright © 2013 Elsevier B.V. All rights reserved.

Combination of ICP-MS, capillary electrophoresis, and their hyphenation for probing Ru(III) metallodrug-DNA interactions.

PubMed

Foteeva, Lidia S; Matczuk, Magdalena; Pawlak, Katarzyna; Aleksenko, Svetlana S; Nosenko, Sergey V; Karandashev, Vasily K; Jarosz, Maciej; Timerbaev, Andrei R

2017-03-01

Determination of the DNA-binding reactivity and affinity is an important part of a successful program for the selection of metallodrug candidates. For such assaying, a range of complementary analytical techniques was proposed and tested here using one of few anticancer metal-based drugs that are currently in clinical trials, indazolium trans-[tetrachloridobis(1H-indazole)ruthenate(III), and a DNA oligonucleotide. A high reactivity of the Ru drug was confirmed in affinity capillary electrophoresis (CE) mode, where adduct formation takes place in situ (i.e., in the capillary filled with an oligonucleotide-containing electrolyte). To further characterize the binding kinetics, a drug-oligonucleotide mixture was incubated for a different period of time, followed by ultrafiltration separation into two different in molecular weight fractions (>3 and <3 kDa). The time-dependent distribution profiles of the Ru drug were then assessed by CE-inductively coupled plasma mass spectrometry (ICP-MS), revealing that at least two DNA adducts exist at equilibrium conditions. Using standalone ICP-MS, dominant equilibrium amount of the bound ruthenium was found to occur in a fraction of 5-10 kDa, which includes the oligonucleotide (ca. 6 kDa). Importantly, in all three assays, the drug was used for the first time in in-vitro studies, not in the intact form but as its active species released from the transferrin adduct at simulated cancer cytosolic conditions. This circumstance makes the established analytical platform promising to provide a detailed view on metallodrug targeting, including other possible biomolecules and ex vivo samples.

DNA migration mechanism analyses for applications in capillary and microchip electrophoresis

PubMed Central

Forster, Ryan E.; Hert, Daniel G.; Chiesl, Thomas N.; Fredlake, Christopher P.; Barron, Annelise E.

2009-01-01

In 2009, electrophoretically driven DNA separations in slab gels and capillaries have the sepia tones of an old-fashioned technology in the eyes of many, even while they remain ubiquitously used, fill a unique niche, and arguably have yet to reach their full potential. For comic relief, what is old becomes new again: agarose slab gel separations are used to prepare DNA samples for “next-gen” sequencing platforms (e.g., the Illumina and 454 machines)—dsDNA molecules within a certain size range are “cut out” of a gel and recovered for subsequent “massively parallel” pyrosequencing. In this review, we give a Barron lab perspective on how our comprehension of DNA migration mechanisms in electrophoresis has evolved, since the first reports of DNA separations by CE (∼1989) until now, 20 years later. Fused silica capillaries, and borosilicate glass and plastic microchips, quietly offer increasing capacities for fast (and even “ultra-fast”), efficient DNA separations. While the channel-by-channel scaling of both old and new electrophoresis platforms provides key flexibility, it requires each unique DNA sample to be prepared in its own micro- or nanovolume. This Achille's heel of electrophoresis technologies left an opening through which pooled-sample, next-gen DNA sequencing technologies rushed. We shall see, over time, whether sharpening understanding of transitions in DNA migration modes in crosslinked gels, nanogel solutions, and uncrosslinked polymer solutions will allow electrophoretic DNA analysis technologies to flower again. Microchannel electrophoresis, after a quiet period of metamorphosis, may emerge sleeker and more powerful, to claim its own important niche applications. PMID:19582705

Capillary waves with surface viscosity

NASA Astrophysics Data System (ADS)

Shen, Li; Denner, Fabian; Morgan, Neal; van Wachem, Berend; Dini, Daniele

2017-11-01

Experiments over the last 50 years have suggested a correlation between the surface (shear) viscosity and the stability of a foam or emulsion. With recent techniques allowing more accurate measurements of the elusive surface viscosity, we examine this link theoretically using small-amplitude capillary waves in the presence of the Marangoni effect and surface viscosity modelled via the Boussinesq-Scriven model. The surface viscosity effect is found to contribute a damping effect on the amplitude of the capillary wave with subtle differences to the effect of the convective-diffusive Marangoni transport. The general wave dispersion is augmented to take into account the Marangoni and surface viscosity effects, and a first-order correction to the critical damping wavelength is derived. The authors acknowledge the financial support of the Shell University Technology Centre for fuels and lubricants.

Hydrophilic solute transport across the rat blood-brain barrier

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lucchesi, K.J.

1987-01-01

Brain capillary permeability-surface area products (PS) of hydrophilic solutes ranging in size from 180 to 5,500 Daltons were measured in rats according to the method of Ohno, Pettigrew and Rapoport. The distribution volume of 70 KD dextran at 10 minutes after i.v. injection was also measured to determine the residual volume of blood in brain tissue at the time of sacrifice. Small test solutes were injected in pairs in order to elucidate whether their transfer into the brain proceeds by diffusion through water- or lipid-filled channels or by vesicular transport. This issue was examined in rats whose blood-brain barrier (BBB)more » was presumed to be intact (untreated) and in rats that received intracarotid infusions to open the BBB (isosmotic salt (ISS) and hyperosmolar arabinose). Ohno PS values of {sup 3}H-inulin and {sup 14}C-L-glucose in untreated rats were found to decrease as the labelling time was lengthened. This was evidence that a rapidly equilibrating compartment exists between blood and brain that renders the Ohno two-compartment model inadequate for computing true transfer rate constants. When the data were reanalyzed using a multi-compartment graphical analysis, solutes with different molecular radii were found to enter the brain at approximately equal rates. Furthermore, unidirectional transport is likely to be initiated by solute adsorption to a glycocalyx coat on the luminal surface of brain capillary endothelium. Apparently, more inulin than L-glucose was adsorbed, which may account for its slightly faster transfer across the BBB. After rats were treated with intracarotid infusions of ISS or hyperosmolar arabinose, solute PS values were significantly increased, but the ratio of PS for each of the solute pairs approached that of their free-diffusion coefficients.« less

Impact of Incremental Perfusion Loss on Oxygen Transport in a Capillary Network Mathematical Model.

PubMed

Fraser, Graham M; Sharpe, Michael D; Goldman, Daniel; Ellis, Christopher G

2015-07-01

To quantify how incremental capillary PL, such as that seen in experimental models of sepsis, affects tissue oxygenation using a computation model of oxygen transport. A computational model was applied to capillary networks with dimensions 84 × 168 × 342 (NI) and 70 × 157 × 268 (NII) μm, reconstructed in vivo from rat skeletal muscle. FCD loss was applied incrementally up to ~40% and combined with high tissue oxygen consumption to simulate severe sepsis. A loss of ~40% FCD loss decreased median tissue PO2 to 22.9 and 20.1 mmHg in NI and NII compared to 28.1 and 27.5 mmHg under resting conditions. Increasing RBC SR to baseline levels returned tissue PO2 to within 5% of baseline. HC combined with a 40% FCD loss, resulted in tissue anoxia in both network volumes and median tissue PO2 of 11.5 and 8.9 mmHg in NI and NII respectively; median tissue PO2 was recovered to baseline levels by increasing total SR 3-4 fold. These results suggest a substantial increase in total SR is required in order to compensate for impaired oxygen delivery as a result of loss of capillary perfusion and increased oxygen consumption during sepsis. © 2015 John Wiley & Sons Ltd.

Occlusion and rupture of ex vivo capillary bifurcation due to acoustic droplet vaporization

NASA Astrophysics Data System (ADS)

Feng, Yi; Qin, Dui; Zhang, Jun; Zhang, Lei; Bouakaz, Ayache; Wan, Mingxi

2018-06-01

Gas embolotherapy (GE) consists in the occlusion of tumor blood vessels using gas emboli induced by acoustic droplet vaporization (ADV), to create tumor starvation and localized drug delivery. Therefore, the occlusion and rupture of capillary bifurcation due to ADV was investigated in an ex vivo rat mesentery model using a confocal acousto-optical high-speed microscope system. Following ADV bubble formation, coalescence, and translational movement, the growing bubbles lodged in and then occluded two different capillary bifurcations. Capillary rupture was induced at the bubble lodging area, immediately followed by gas extravasation and bubble dislodging. Before and after bubble lodgment/occlusion, a local microvessel invagination was observed due to the interactions between ADV bubbles and the microvessel itself, indicating a contribution to the capillary rupture. Understanding the transient dynamics of ADV bubble, the bubble-microvessel interaction and the consequent mechanical bio-effects in GE is of the paramount importance for developing and applying this approach in clinical practice.

A replaceable microreactor for on-line protein digestion in a two-dimensional capillary electrophoresis system with tandem mass spectrometry detection

PubMed Central

Li, Yihan; Wojcik, Roza; Dovichi, Norman J.

2010-01-01

We describe a two-dimensional capillary electrophoresis system that incorporates a replaceable enzymatic microreactor for on-line protein digestion. In this system, trypsin is immobilized on magnetic beads. At the start of each experiment, old beads are flushed to waste and replaced with a fresh plug of beads, which is captured by a pair of magnets at the distal tip of the first capillary. For analysis, proteins are separated in the first capillary. A fraction is then parked in the reactor to create peptides. Digested peptides are periodically transferred to the second capillary for separation; a fresh protein fraction is simultaneously moved to the reactor for digestion. An electrospray interface is used to introduce peptides into a mass spectrometer for analysis. This procedure is repeated for several dozen fractions under computer control. The system was demonstrated by the separation and digestion of insulin chain b oxidized and β-casein as model proteins. PMID:21030030

A FORTRAN program for interpretation of relative permeability from unsteady-state displacements with capillary pressure included

USGS Publications Warehouse

Udegbunam, E.O.

1991-01-01

This paper presents a FORTRAN program for the determination of two-phase relative permeabilities from unsteady-state displacement data with capillary pressure terms included. The interpretative model employed in this program combines the simultaneous solution of a variant of the fractional flow equation which includes a capillary pressure term and an integro-differential equation derived from Darcy's law without assuming the simplified Buckley-Leverett flow. The incorporation of capillary pressure in the governing equations dispenses with the high flowrate experimental requirements normally employed to overcome capillarity effects. An illustrative example is presented herein which implements this program for the determination of oil/water relative permeabilities from a sandstone core sample. Results obtained compares favorably with results previously given in the literature. ?? 1991.

Gas adsorption and capillary condensation in nanoporous alumina films.

PubMed

Casanova, Fèlix; Chiang, Casey E; Li, Chang-Peng; Roshchin, Igor V; Ruminski, Anne M; Sailor, Michael J; Schuller, Ivan K

2008-08-06

Gas adsorption and capillary condensation of organic vapors are studied by optical interferometry, using anodized nanoporous alumina films with controlled geometry (cylindrical pores with diameters in the range of 10-60 nm). The optical response of the film is optimized with respect to the geometric parameters of the pores, for potential performance as a gas sensor device. The average thickness of the adsorbed film at low relative pressures is not affected by the pore size. Capillary evaporation of the liquid from the nanopores occurs at the liquid-vapor equilibrium described by the classical Kelvin equation with a hemispherical meniscus. Due to the almost complete wetting, we can quantitatively describe the condensation for isopropanol using the Cohan model with a cylindrical meniscus in the Kelvin equation. This model describes the observed hysteresis and allows us to use the adsorption branch of the isotherm to calculate the pore size distribution of the sample in good agreement with independent structural measurements. The condensation for toluene lacks reproducibility due to incomplete surface wetting. This exemplifies the relevant role of the fluid-solid (van der Waals) interactions in the hysteretic behavior of capillary condensation.

Modelling mass and heat transfer in nano-based cancer hyperthermia.

PubMed

Nabil, M; Decuzzi, P; Zunino, P

2015-10-01

We derive a sophisticated mathematical model for coupled heat and mass transport in the tumour microenvironment and we apply it to study nanoparticle delivery and hyperthermic treatment of cancer. The model has the unique ability of combining the following features: (i) realistic vasculature; (ii) coupled capillary and interstitial flow; (iii) coupled capillary and interstitial mass transfer applied to nanoparticles; and (iv) coupled capillary and interstitial heat transfer, which are the fundamental mechanisms governing nano-based hyperthermic treatment. This is an improvement with respect to previous modelling approaches, where the effect of blood perfusion on heat transfer is modelled in a spatially averaged form. We analyse the time evolution and the spatial distribution of particles and temperature in a tumour mass treated with superparamagnetic nanoparticles excited by an alternating magnetic field. By means of numerical experiments, we synthesize scaling laws that illustrate how nano-based hyperthermia depends on tumour size and vascularity. In particular, we identify two distinct mechanisms that regulate the distribution of particle and temperature, which are characterized by perfusion and diffusion, respectively.

Effect of Marangoni Convection on Surfactant Transfer Between the Drop Connected to the Reservoir and Surrounding Liquid

NASA Astrophysics Data System (ADS)

Kostarev, K.; Denisova, M.; Shmyrov, A.

2018-03-01

The paper presents the results of comparative investigation of the interaction between the capillary and buoyant mechanisms of motion in a problem of surfactant mass transfer between an insoluble drop and surrounding fluid under different gravity conditions. The research was performed for the drop that is coupled with the reservoir filled with a source mixture through a long thin tube (needle). Visualization of the flow patterns and concentration fields has shown that surfactant diffusion from the needle at normal gravity leads to the onset of the oscillatory mode of the capillary convection in the drop. It has been found that the frequency of the Marangoni convection outbursts, the lifetime of the oscillatory flow modes and the amount of the source mixture involved in the process of mass transfer depend on the drop size and initial concentration of the surfactant. The obtained results are compared with the cases of surfactant diffusion from the isolated drop under terrestrial conditions and from the drop coupled with reservoir in microgravity. Additionally, a series of experiments were performed to investigate diffusion of a surfactant from the surrounding solution into a drop.

Sorptivity of rocks and soils of the van Genuchten-Mualem type

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zimmerman, R.W.; Bodvarsson, G.S.

1991-06-01

One hydrological process that will have great relevance to the performance of the proposed underground radioactive waste repository at Yucca Mountain, Nevada, is that of the absorption of water from a water-filled fracture into the adjacent unsaturated rock formation. The rate at which water is imbibed by a rock depends on the hydrological properties of the rock and on the initial saturation (or initial capillary suction) of the formation. The hydrological properties that affect imbibition are the relative permeability function and the capillary pressure function. These functions are often collectively referred to as the `characteristic functions` of the porous medium.more » For one-dimensional absorption, it can be shown that, regardless of the details of the characteristic functions, the total amount of water imbibed by the formation, per unit surface area, will be proportional to the square root of the elapsed time. Hence the ability of a rock or soil to imbibe water can be quantified by a parameter known as the sorptivity S, which is defined such that the cumulative volumetric liquid influx per unit area is given by Q = S{radical}t. The paper discusses the simplification of these characteristic functions of porous medium.« less

Stabilization of a capillary bridge far beyond the Rayleigh--Plateau limit using active feedback and acoustic radiation pressure.

NASA Astrophysics Data System (ADS)

Marr-Lyon, Mark J.; Thiessen, David B.; Marston, Philip L.

1997-11-01

A liquid bridge between two solid surfaces is known as a capillary bridge. For a cylindrical bridge in low gravity of radius R and length L, the slenderness S=L/2R has a natural (Rayleigh--Plateau) limit of π beyond which the bridge breaks. Using the radiation pressure of an ultrasonic standing wave to control the shape of the bridge and an optical sensor to detect the shape of the bridge, an active feedback system was constructed that stabilized bridges significantly beyond the Rayleigh limit in simulated low gravity(Marr--Lyon, M. J., phet al., J. Fluid Mech.), accepted for publication.. The Plateau tank which contained the bridge was a dual frequency ultrasonic resonator and the spatial distribution of the radiation pressure was controlled by adjusting the ultrasonic frequency. Bridges have been extended with S as large as 4.3. To be useful in low gravity, modifications for liquid bridges in air are needed. Acoustic resonators in air having the required property that the sound amplitude can be spatially redistributed rapidly are being investigated using gas-filled soap-film bridges. Work supported by NASA.

Functional integration of PCR amplification and capillary eletrophoresis in a microfabricated DNA analysis device

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woolley, A.T.; deMello, A.J.; Mathies, R.A.

Microfabricated silicon PCR reactors and glass capillary electrophoresis (CE) chips have been successfully coupled to form an integrated DNA analysis system. This construct combines the rapid thermal cycling capabilities of microfabricated PCR devices (10{degree}C/s heating, 2.5{degree}C/s cooling) with the high-speed (<120 s) DNA separations provided by microfabricated CE chips. The PCR chamber and the CE chip were directly linked through a photolithographically fabricated channel filled with hydroxyethylcellulose sieving matrix. Electrophoretic injection directly from the PCR chamber through the cross injection channel was used as an `electrophoretic valve` to couple the PCR and CE devices on-chip. To demonstrate the functionality ofmore » this system, a 15 min PCR amplification of a {Beta}-globin target cloned in m13 was immediately followed by high-speed CE chip separation in under 120 s, providing a rapid PCR-CE analysis in under 20 min. A rapid assay for genomic Salmonella DNA was performed in under 45 min, demonstrating that challenging amplifications of diagnostically interesting targets can also be performed. Real-time monitoring of PCR target amplification in these integrated PCR-CE devices is also feasible. 33 refs., 6 figs.« less

A Study of Crystalline Mechanism of Penetration Sealer Materials.

PubMed

Teng, Li-Wei; Huang, Ran; Chen, Jie; Cheng, An; Hsu, Hui-Mi

2014-01-14

It is quite common to dispense a topping material like crystalline penetration sealer materials (CPSM) onto the surface of a plastic substance such as concrete to extend its service life span by surface protections from outside breakthrough. The CPSM can penetrate into the existing pores or possible cracks in such a way that it may form crystals to block the potential paths which provide breakthrough for any unknown materials. This study investigated the crystalline mechanism formed in the part of concrete penetrated by the CPSM. We analyzed the chemical composites, in order to identify the mechanism of CPSM and to evaluate the penetrated depth. As shown in the results, SEM observes the acicular-structured crystals filling capillary pores for mortar substrate of the internal microstructure beneath the concrete surface; meanwhile, XRD and FT-IR showed the main hydration products of CPSM to be C-S-H gel and CaCO₃. Besides, MIP also shows CPSM with the ability to clog capillary pores of mortar substrate; thus, it reduces porosity, and appears to benefit in sealing pores or cracks. The depth of CPSM penetration capability indicated by TGA shows 0-10 mm of sealer layer beneath the concrete surface.

The influence of surfactant on the propagation of a semi-infinite bubble through a liquid-filled compliant channel

PubMed Central

Halpern, David; Gaver, Donald P.

2012-01-01

We investigate the influence of a soluble surfactant on the steady-state motion of a finger of air through a compliant channel. This study provides a basic model from which to understand the fluid–structure interactions and physicochemical hydrodynamics of pulmonary airway reopening. Airway closure occurs in lung diseases such as respiratory distress syndrome and acute respiratory distress syndrome as a result of fluid accumulation and surfactant insufficiency. This results in ‘compliant collapse’ with the airway walls buckled and held in apposition by a liquid occlusion that blocks the passage of air. Airway reopening is essential to the recovery of adequate ventilation, but has been associated with ventilator-induced lung injury because of the exposure of airway epithelial cells to large interfacial flow-induced pressure gradients. Surfactant replacement is helpful in modulating this deleterious mechanical stimulus, but is limited in its effectiveness owing to slow surfactant adsorption. We investigate the effect of surfactant on micro-scale models of reopening by computationally modelling the steady two-dimensional motion of a semi-infinite bubble propagating through a liquid-filled compliant channel doped with soluble surfactant. Many dimensionless parameters affect reopening, but we primarily investigate how the reopening pressure pb depends upon the capillary number Ca (the ratio of viscous to surface tension forces), the adsorption depth parameter λ (a bulk concentration parameter) and the bulk Péclet number Peb (the ratio of bulk convection to diffusion). These studies demonstrate a dependence of pb on λ, and suggest that a critical bulk concentration must be exceeded to operate as a low-surface-tension system. Normal and tangential stress gradients remain largely unaffected by physicochemical interactions – for this reason, further biological studies are suggested that will clarify the role of wall flexibility and surfactant on the protection of the lung from atelectrauma. PMID:22997476

On the performance of capillary barriers as landfill cover

NASA Astrophysics Data System (ADS)

Kämpf, M.; Montenegro, H.

Landfills and waste heaps require an engineered surface cover upon closure. The capping system can vary from a simple soil cover to multiple layers of earth and geosynthetic materials. Conventional design features a compacted soil layer, which suffers from drying out and cracking, as well as root and animal intrusion. Capillary barriers consisting of inclined fine-over-coarse soil layers are investigated as an alternative cover system. Under unsaturated conditions, the textural contrast delays vertical drainage by capillary forces. The moisture that builds up above the contact will flow downdip along the interface of the layers. Theoretical studies of capillary barriers have identified the hydraulic properties of the layers, the inclination angle, the length of the field and the infiltration rate as the fundamental characteristics of the system. However, it is unclear how these findings can lead to design criteria for capillary barriers. To assess the uncertainty involved in such approaches, experiments have been carried out in a 8 m long flume and on large scale test sites (40 m x 15 m). In addition, the ability of a numerical model to represent the relevant flow processes in capillary barriers has been examined.

On-line wall-free cell for laser-induced fluorescence detection in capillary electrophoresis.

PubMed

Yu, Chang-Zhu; He, You-Zhao; Xie, Hai-Yang; Gao, Yong; Gan, Wu-Er; Li, Jun

2009-05-15

A wall-free detection method based on liquid junction in a capillary gap was proposed for laser-induced fluorescence (LIF) of capillary electrophoresis (CE). The capillary gap of the wall-free cell was fabricated by etching a 10-mm x 50-microm I.D. fused-silica capillary to obtain a polyimide coating sleeve, decoating about 6mm at one end of both 50 microm I.D. separation and liquid junction capillary, inserting the treated capillary ends into the coating sleeve oppositely, fixing the capillaries with a gap distance of 140 microm by epoxy glue and removing the coating sleeve by burning. The theoretical model, experimental results and wall-free cell images indicated that the gap distance and applied voltage were main influence factors on the wall-free detection. Since the wall-free cell increased the absorption light path and avoided the stray light from the capillary wall, it improved the ratio of signal to noise and limit of detection (LOD) of CE-LIF. Three flavin compounds of riboflavin (RF), flavin mononucleotide sodium (FMN) and flavin adenine dinucleotide disodium (FAD) were used to evaluate the wall-free detection method. Compared with on-column cell, the LODs of the wall-free cell were improved 15-, 6- and 9-fold for RF, FMN and FAD, respectively. The linear calibration concentrations of the flavins ranged from 0.005 to 5.0 micromol/L. The column efficiency was in the range from 1.0 x 10(5) to 2.5 x 10(5) plates. The wall-free detection of CE-LIF was applied to the analysis of the flavins in spinach and lettuce leaves.

Combined contactless conductometric, photometric, and fluorimetric single point detector for capillary separation methods.

PubMed

Ryvolová, Markéta; Preisler, Jan; Foret, Frantisek; Hauser, Peter C; Krásenský, Pavel; Paull, Brett; Macka, Mirek

2010-01-01

This work for the first time combines three on-capillary detection methods, namely, capacitively coupled contactless conductometric (C(4)D), photometric (PD), and fluorimetric (FD), in a single (identical) point of detection cell, allowing concurrent measurements at a single point of detection for use in capillary electrophoresis, capillary electrochromatography, and capillary/nanoliquid chromatography. The novel design is based on a standard 6.3 mm i.d. fiber-optic SMA adapter with a drilled opening for the separation capillary to go through, to which two concentrically positioned C(4)D detection electrodes with a detection gap of 7 mm were added on each side acting simultaneously as capillary guides. The optical fibers in the SMA adapter were used for the photometric signal (absorbance), and another optical fiber at a 45 degrees angle to the capillary was applied to collect the emitted light for FD. Light emitting diodes (255 and 470 nm) were used as light sources for the PD and FD detection modes. LOD values were determined under flow-injection conditions to exclude any stacking effects: For the 470 nm LED limits of detection (LODs) for FD and PD were for fluorescein (1 x 10(-8) mol/L) and tartrazine (6 x 10(-6) mol/L), respectively, and the LOD for the C(4)D was for magnesium chloride (5 x 10(-7) mol/L). The advantage of the three different detection signals in a single point is demonstrated in capillary electrophoresis using model mixtures and samples including a mixture of fluorescent and nonfluorescent dyes and common ions, underivatized amino acids, and a fluorescently labeled digest of bovine serum albumin.

Scale-dependent coupling of hysteretic capillary pressure, trapping, and fluid mobilities

NASA Astrophysics Data System (ADS)

Doster, F.; Celia, M. A.; Nordbotten, J. M.

2012-12-01

Many applications of multiphase flow in porous media, including CO2-storage and enhanced oil recovery, require mathematical models that span a large range of length scales. In the context of numerical simulations, practical grid sizes are often on the order of tens of meters, thereby de facto defining a coarse model scale. Under particular conditions, it is possible to approximate the sub-grid-scale distribution of the fluid saturation within a grid cell; that reconstructed saturation can then be used to compute effective properties at the coarse scale. If both the density difference between the fluids and the vertical extend of the grid cell are large, and buoyant segregation within the cell on a sufficiently shorte time scale, then the phase pressure distributions are essentially hydrostatic and the saturation profile can be reconstructed from the inferred capillary pressures. However, the saturation reconstruction may not be unique because the parameters and parameter functions of classical formulations of two-phase flow in porous media - the relative permeability functions, the capillary pressure -saturation relationship, and the residual saturations - show path dependence, i.e. their values depend not only on the state variables but also on their drainage and imbibition histories. In this study we focus on capillary pressure hysteresis and trapping and show that the contribution of hysteresis to effective quantities is dependent on the vertical length scale. By studying the transition from the two extreme cases - the homogeneous saturation distribution for small vertical extents and the completely segregated distribution for large extents - we identify how hysteretic capillary pressure at the local scale induces hysteresis in all coarse-scale quantities for medium vertical extents and finally vanishes for large vertical extents. Our results allow for more accurate vertically integrated modeling while improving our understanding of the coupling of capillary pressure and relative permeabilities over larger length scales.

Agglomeration of Non-metallic Inclusions at Steel/Ar Interface: In- Situ Observation Experiments and Model Validation

NASA Astrophysics Data System (ADS)

Mu, Wangzhong; Dogan, Neslihan; Coley, Kenneth S.

2017-10-01

Better understanding of agglomeration behavior of nonmetallic inclusions in the steelmaking process is important to control the cleanliness of the steel. In this work, a revision on the Paunov simplified model has been made according to the original Kralchevsky-Paunov model. Thus, this model has been applied to quantitatively calculate the attractive capillary force on inclusions agglomerating at the liquid steel/gas interface. Moreover, the agglomeration behavior of Al2O3 inclusions at a low carbon steel/Ar interface has been observed in situ by high-temperature confocal laser scanning microscopy (CLSM). The velocity and acceleration of inclusions and attractive forces between Al2O3 inclusions of various sizes were calculated based on the CLSM video. The results calculated using the revised model offered a reasonable fit with the present experimental data for different inclusion sizes. Moreover, a quantitative comparison was made between calculations using the equivalent radius of a circle and those using the effective radius. It was found that the calculated capillary force using equivalent radius offered a better fit with the present experimental data because of the inclusion characteristics. Comparing these results with other studies in the literature allowed the authors to conclude that when applied in capillary force calculations, the equivalent radius is more suitable for inclusions with large size and irregular shape, and the effective radius is more appropriate for inclusions with small size or a large shape factor. Using this model, the effect of inclusion size on attractive capillary force has been investigated, demonstrating that larger inclusions are more strongly attracted.

Estimation of blood flow heterogeneity in human skeletal muscle using intravascular tracer data: importance for modeling transcapillary exchange.

PubMed

Vicini, P; Bonadonna, R C; Lehtovirta, M; Groop, L C; Cobelli, C

1998-01-01

Distributed models of blood-tissue exchange are widely used to measure kinetic events of various solutes from multiple tracer dilution experiments. Their use requires, however, a careful description of blood flow heterogeneity along the capillary bed. Since they have mostly been applied in animal studies, direct measurement of the heterogeneity distribution was possible, e.g., with the invasive microsphere method. Here we apply distributed modeling to a dual tracer experiment in humans, performed using an intravascular (indocyanine green dye, subject to distribution along the vascular tree and confined to the capillary bed) and an extracellular ([3H]-D-mannitol, tracing passive transcapillary transfer across the capillary membrane in the interstitial fluid) tracer. The goal is to measure relevant parameters of transcapillary exchange in human skeletal muscle. We show that assuming an accurate description of blood flow heterogeneity is crucial for modeling, and in particular that assuming for skeletal muscle the well-studied cardiac muscle blood flow heterogeneity is inappropriate. The same reason prevents the use of the common method of estimating the input function of the distributed model via deconvolution, which assumes a known blood flow heterogeneity, either defined from literature or measured, when possible. We present a novel approach for the estimation of blood flow heterogeneity in each individual from the intravascular tracer data. When this newly estimated blood flow heterogeneity is used, a more satisfactory model fit is obtained and it is possible to reliably measure parameters of capillary membrane permeability-surface product and interstitial fluid volume describing transcapillary transfer in vivo.

Monte Carlo calculation of the maximum therapeutic gain of tumor antivascular alpha therapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang, Chen-Yu; Oborn, Bradley M.; Guatelli, Susanna

Purpose: Metastatic melanoma lesions experienced marked regression after systemic targeted alpha therapy in a phase 1 clinical trial. This unexpected response was ascribed to tumor antivascular alpha therapy (TAVAT), in which effective tumor regression is achieved by killing endothelial cells (ECs) in tumor capillaries and, thus, depriving cancer cells of nutrition and oxygen. The purpose of this paper is to quantitatively analyze the therapeutic efficacy and safety of TAVAT by building up the testing Monte Carlo microdosimetric models. Methods: Geant4 was adapted to simulate the spatial nonuniform distribution of the alpha emitter {sup 213}Bi. The intraluminal model was designed tomore » simulate the background dose to normal tissue capillary ECs from the nontargeted activity in the blood. The perivascular model calculates the EC dose from the activity bound to the perivascular cancer cells. The key parameters are the probability of an alpha particle traversing an EC nucleus, the energy deposition, the lineal energy transfer, and the specific energy. These results were then applied to interpret the clinical trial. Cell survival rate and therapeutic gain were determined. Results: The specific energy for an alpha particle hitting an EC nucleus in the intraluminal and perivascular models is 0.35 and 0.37 Gy, respectively. As the average probability of traversal in these models is 2.7% and 1.1%, the mean specific energy per decay drops to 1.0 cGy and 0.4 cGy, which demonstrates that the source distribution has a significant impact on the dose. Using the melanoma clinical trial activity of 25 mCi, the dose to tumor EC nucleus is found to be 3.2 Gy and to a normal capillary EC nucleus to be 1.8 cGy. These data give a maximum therapeutic gain of about 180 and validate the TAVAT concept. Conclusions: TAVAT can deliver a cytotoxic dose to tumor capillaries without being toxic to normal tissue capillaries.« less

Diffusive oxygen shunting between vessels in the preglomerular renal vasculature: anatomic observations and computational modeling.

PubMed

Gardiner, Bruce S; Thompson, Sarah L; Ngo, Jennifer P; Smith, David W; Abdelkader, Amany; Broughton, Brad R S; Bertram, John F; Evans, Roger G

2012-09-01

To understand how geometric factors affect arterial-to-venous (AV) oxygen shunting, a mathematical model of diffusive oxygen transport in the renal cortex was developed. Preglomerular vascular geometry was investigated using light microscopy (providing vein shape, AV separation, and capillary density near arteries) and published micro-computed tomography (CT) data (providing vessel size and AV separation; Nordsletten DA, Blackett S, Bentley MD, Ritman EL, Smith NP. IUPS Physiome Project. http://www.physiome.org.nz/publications/nordsletten_blackett_ritman_bentley_smith_2005/folder_contents). A "U-shaped" relationship was observed between the arterial radius and the distance between the arterial and venous lumens. Veins were found to partially wrap around the artery more consistently for larger rather than smaller arteries. Intrarenal arteries were surrounded by an area of fibrous tissue, lacking capillaries, the thickness of which increased from ∼5 μm for the smallest arteries (<16-μm diameter) to ∼20 μm for the largest arteries (>200-μm diameter). Capillary density was greater near smaller arteries than larger arteries. No capillaries were observed between wrapped AV vessel pairs. The computational model comprised a single AV pair in cross section. Geometric parameters critical in renal oxygen transport were altered according to variations observed by CT and light microscopy. Lumen separation and wrapping of the vein around the artery were found to be the critical geometric factors determining the amount of oxygen shunted between AV pairs. AV oxygen shunting increases both as lumen separation decreases and as the degree of wrapping increases. The model also predicts that capillaries not only deliver oxygen, but can also remove oxygen from the cortical parenchyma close to an AV pair. Thus the presence of oxygen sinks (capillaries or tubules) near arteries would reduce the effectiveness of AV oxygen shunting. Collectively, these data suggest that AV oxygen shunting would be favored in larger vessels common to the cortical and medullary circulations (i.e., arcuate and proximal interlobular arteries) rather than the smaller vessels specific to the cortical circulation (distal interlobular arteries and afferent arterioles).

Core analysis of heterogeneous rocks using experimental observations and digital whole core simulation

NASA Astrophysics Data System (ADS)

Jackson, S. J.; Krevor, S. C.; Agada, S.

2017-12-01

A number of studies have demonstrated the prevalent impact that small-scale rock heterogeneity can have on larger scale flow in multiphase flow systems including petroleum production and CO2sequestration. Larger scale modeling has shown that this has a significant impact on fluid flow and is possibly a significant source of inaccuracy in reservoir simulation. Yet no core analysis protocol has been developed that faithfully represents the impact of these heterogeneities on flow functions used in modeling. Relative permeability is derived from core floods performed at conditions with high flow potential in which the impact of capillary heterogeneity is voided. A more accurate representation would be obtained if measurements were made at flow conditions where the impact of capillary heterogeneity on flow is scaled to be representative of the reservoir system. This, however, is generally impractical due to laboratory constraints and the role of the orientation of the rock heterogeneity. We demonstrate a workflow of combined observations and simulations, in which the impact of capillary heterogeneity may be faithfully represented in the derivation of upscaled flow properties. Laboratory measurements that are a variation of conventional protocols are used for the parameterization of an accurate digital rock model for simulation. The relative permeability at the range of capillary numbers relevant to flow in the reservoir is derived primarily from numerical simulations of core floods that include capillary pressure heterogeneity. This allows flexibility in the orientation of the heterogeneity and in the range of flow rates considered. We demonstrate the approach in which digital rock models have been developed alongside core flood observations for three applications: (1) A Bentheimer sandstone with a simple axial heterogeneity to demonstrate the validity and limitations of the approach, (2) a set of reservoir rocks from the Captain sandstone in the UK North Sea targeted for CO2 storage, and for which the use of capillary pressure hysteresis is necessary, and (3) a secondary CO2-EOR production of residual oil from a Berea sandstone with layered heterogeneities. In all cases the incorporation of heterogeneity is shown to be key to the ultimate derivation of flow properties representative of the reservoir system.

Numerical analysis of cell adhesion in capillary flow

NASA Astrophysics Data System (ADS)

Takeishi, Naoki; Imai, Yohsuke; Ishida, Shunichi; Omori, Toshihiro; Kamm, Roger; Ishikawa, Takuji

2016-11-01

Numerical simulation of cell adhesion was performed for capillaries whose diameter is comparable to or smaller than that of the cell. Despite a lot of works about leukocyte and tumor cell rolling, cell motion in capillaries has remained unclear. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to "bullet-like" motion, with a consequent decrease in the velocity of the cell. A state diagram is obtained for various values of capillary diameter and receptor density. According to our numerical results, bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between PSGL-1 and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis. This research was supported by JSPS KAKENHI Grant Numbers 25000008, 26107703, 14J03967. We also acknowledge support from the Tohoku University Division for International Advanced Research and Education Organization.

Viscosity of particulate soap films: approaching the jamming of 2D capillary suspensions.

PubMed

Timounay, Yousra; Rouyer, Florence

2017-05-14

We compute the effective viscosity of particulate soap films thanks to local velocity fields obtained by Particle Image Velocimetry (PIV) during film retraction experiments. We identify the jamming of these 2D capillary suspensions at a critical particle surface fraction (≃0.84) where effective viscosity diverges. Pair correlation function and number of neighbors in contact or close to contact reveal the cohesive nature of this 2D capillary granular media. The experimental 2D dynamic viscosities can be predicted by a model considering viscous dissipation at the liquid interfaces induced by the motion of individual particles.

On the consistency of scale among experiments, theory, and simulation

DOE PAGES

McClure, James E.; Dye, Amanda L.; Miller, Cass T.; ...

2017-02-20

As a tool for addressing problems of scale, we consider an evolving approach known as the thermodynamically constrained averaging theory (TCAT), which has broad applicability to hydrology. We consider the case of modeling of two-fluid-phase flow in porous media, and we focus on issues of scale as they relate to various measures of pressure, capillary pressure, and state equations needed to produce solvable models. We apply TCAT to perform physics-based data assimilation to understand how the internal behavior influences the macroscale state of two-fluid porous medium systems. A microfluidic experimental method and a lattice Boltzmann simulation method are used to examinemore » a key deficiency associated with standard approaches. In a hydrologic process such as evaporation, the water content will ultimately be reduced below the irreducible wetting-phase saturation determined from experiments. This is problematic since the derived closure relationships cannot predict the associated capillary pressures for these states. Here, we demonstrate that the irreducible wetting-phase saturation is an artifact of the experimental design, caused by the fact that the boundary pressure difference does not approximate the true capillary pressure. Using averaging methods, we compute the true capillary pressure for fluid configurations at and below the irreducible wetting-phase saturation. Results of our analysis include a state function for the capillary pressure expressed as a function of fluid saturation and interfacial area.« less

On the consistency of scale among experiments, theory, and simulation

NASA Astrophysics Data System (ADS)

McClure, James E.; Dye, Amanda L.; Miller, Cass T.; Gray, William G.

2017-02-01

As a tool for addressing problems of scale, we consider an evolving approach known as the thermodynamically constrained averaging theory (TCAT), which has broad applicability to hydrology. We consider the case of modeling of two-fluid-phase flow in porous media, and we focus on issues of scale as they relate to various measures of pressure, capillary pressure, and state equations needed to produce solvable models. We apply TCAT to perform physics-based data assimilation to understand how the internal behavior influences the macroscale state of two-fluid porous medium systems. A microfluidic experimental method and a lattice Boltzmann simulation method are used to examine a key deficiency associated with standard approaches. In a hydrologic process such as evaporation, the water content will ultimately be reduced below the irreducible wetting-phase saturation determined from experiments. This is problematic since the derived closure relationships cannot predict the associated capillary pressures for these states. We demonstrate that the irreducible wetting-phase saturation is an artifact of the experimental design, caused by the fact that the boundary pressure difference does not approximate the true capillary pressure. Using averaging methods, we compute the true capillary pressure for fluid configurations at and below the irreducible wetting-phase saturation. Results of our analysis include a state function for the capillary pressure expressed as a function of fluid saturation and interfacial area.

Modeling the Capillary Pressure for the Migration of the Liquid Phase in Granular Solid-Liquid-Vapor Systems: Application to the Control of the Composition Profile in W-Cu FGM Materials

NASA Astrophysics Data System (ADS)

Missiaen, Jean-Michel; Raharijaona, Jean-Joël; Delannay, Francis

2016-11-01

A model is developed to compute the capillary pressure for the migration of the liquid phase out or into a uniform solid-liquid-vapor system. The capillary pressure is defined as the reduction of the overall interface energy per volume increment of the transferred fluid phase. The model takes into account the particle size of the solid particle aggregate, the packing configuration (coordination number, porosity), the volume fractions of the different phases, and the values of the interface energies in the system. The model is used for analyzing the stability of the composition profile during processing of W-Cu functionally graded materials combining a composition gradient with a particle size gradient. The migration pressure is computed with the model in two stages: (1) just after the melting of copper, i.e., when sintering and shape accommodation of the W particle aggregate can still be neglected and (2) at high temperature, when the system is close to full density with equilibrium particle shape. The model predicts well the different stages of liquid-phase migration observed experimentally.

Improvements to water vapor transmission and capillary absorption measurements in porous materials

Treesearch

Samuel L. Zelinka; Samuel V. Glass; Charles R. Boardman

2016-01-01

The vapor permeability (or equivalently the vapor diffusion resistance factor) and the capillary absorption coefficient are frequently used as inputs to hygrothermal or heat, air, and moisture (HAM) models. However, it has been well documented that the methods used to determine these properties are sensitive to the operator, and wide variations in the properties have...

Modeling Wettability Alteration using Chemical EOR Processes in Naturally Fractured Reservoirs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mojdeh Delshad; Gary A. Pope; Kamy Sepehrnoori

2007-09-30

The objective of our search is to develop a mechanistic simulation tool by adapting UTCHEM to model the wettability alteration in both conventional and naturally fractured reservoirs. This will be a unique simulator that can model surfactant floods in naturally fractured reservoir with coupling of wettability effects on relative permeabilities, capillary pressure, and capillary desaturation curves. The capability of wettability alteration will help us and others to better understand and predict the oil recovery mechanisms as a function of wettability in naturally fractured reservoirs. The lack of a reliable simulator for wettability alteration means that either the concept that hasmore » already been proven to be effective in the laboratory scale may never be applied commercially to increase oil production or the process must be tested in the field by trial and error and at large expense in time and money. The objective of Task 1 is to perform a literature survey to compile published data on relative permeability, capillary pressure, dispersion, interfacial tension, and capillary desaturation curve as a function of wettability to aid in the development of petrophysical property models as a function of wettability. The new models and correlations will be tested against published data. The models will then be implemented in the compositional chemical flooding reservoir simulator, UTCHEM. The objective of Task 2 is to understand the mechanisms and develop a correlation for the degree of wettability alteration based on published data. The objective of Task 3 is to validate the models and implementation against published data and to perform 3-D field-scale simulations to evaluate the impact of uncertainties in the fracture and matrix properties on surfactant alkaline and hot water floods.« less

Model of formation of droplets during electric arc surfacing of functional coatings

NASA Astrophysics Data System (ADS)

Sarychev, Vladimir D.; Granovskii, Alexei Yu; Nevskii, Sergey A.; Gromov, Victor E.

2016-01-01

The mathematical model was developed for the initial stage of formation of an electrode metal droplet in the process of arc welding. Its essence lies in the fact that the presence of a temperature gradient in the boundary layer of the molten metal causes thermo-capillary instability, which leads to the formation of electrode metal droplets. A system of equations including Navier-Stokes equations, heat conduction and Maxwell's equations was solved as well as the boundary conditions for the system electrodes-plasma. Dispersion equation for thermo-capillary waves in the linear approximation for the plane layer was received and analyzed. The values of critical wavelengths, at which thermo-capillary instability appears in the nanometer wavelength range, were found. The parameters at which the mode of a fine-droplet transfer of the material takes place were theoretically defined.

Interaction between blood and solid particles propagating through a capillary with slip effects.

PubMed

Zeeshan, A; Fatima, A; Khalid, F; Bhatti, M M

2018-04-18

This article describes the interaction between solid particles and blood propagating through a capillary. A slip condition is considered on the walls of the capillary. The rheological features of the blood are discussed by considering as a two-phase Newtonian fluid model, i.e., the suspension of cells in plasma. A perturbation method is successfully applied to obtain the series solution of the governing coupled differential equations. The series solution for both fluid and particle phase are presented up to second order approximation. The expressions for the velocity and pressure distributions under slip effects are determined within a tube. Furthermore, the current results are beneficial to understand the rheological features of blood which will be helpful to interpret and analyze more complex blood flow models. Copyright © 2018 Elsevier Inc. All rights reserved.

Characterization of heterogeneity in the Heletz sandstone from core to pore scale and quantification of its impact on multi-phase flow

DOE PAGES

Hingerl, Ferdinand F.; Yang, Feifei; Pini, Ronny; ...

2016-02-02

In this paper we present the results of an extensive multiscale characterization of the flow properties and structural and capillary heterogeneities of the Heletz sandstone. We performed petrographic, porosity and capillary pressure measurements on several subsamples. We quantified mm-scale heterogeneity in saturation distributions in a rock core during multi-phase flow using conventional X-ray CT scanning. Core-flooding experiments were conducted under reservoirs conditions (9 MPa, 50 °C) to obtain primary drainage and secondary imbibition relative permeabilities and residual trapping was analyzed and quantified. We provide parameters for relative permeability, capillary pressure and trapping models for further modeling studies. A synchrotron-based microtomographymore » study complements our cm- to mm-scale investigation by providing links between the micromorphology and mm-scale saturation heterogeneities.« less

Migration And Entrapment Of Mercury In The Subsurface

NASA Astrophysics Data System (ADS)

M, D.; Nambi, I. M.

2009-12-01

Elemental mercury is an immiscible liquid with high density and high surface tension. The movement of mercury in the saturated subsurface region is therefore considered a case of two phase flow involving mercury and water and is expected to be governed by gravity, viscous and capillary forces. Fundamental investigation into the migration and capillary entrapment of mercury in the subsurface was done by controlled laboratory capillary pressure saturation experiments using mercury and water as non wetting and wetting fluid respectively. Residual mercury saturation and van Genuchten’s capillary entrapment parameters were determined independently for different sizes of porous media. Based on the experimental data, theoretical investigations were done on the role of the three predominant forces and their influence on mercury migration and entrapment. The effects of fluid density and interfacial tension and the influence of Capillary and Bond number on mercury entrapment were analyzed with the help of similar capillary pressure - saturation experiments using Tetrachloroethylene (PCE)-water fluid pair. Mercury-water systems exhibited a low residual saturation of 0.02 and 0.07 as compared to 0.16 and 0.27 for PCE-water systems. Less residual mercury saturation, lack of apparent hysteresis in capillary pressure saturation curves and large variation in van Genuchten’s parameters 'α'(inverse of displacement pressure) and ‘n’ (pore size distribution index) for mercury-water systems compared to PCE-water systems were observed. These anomalies between the two systems elucidate that the capillary trapping is equally dependent on the fluid characteristics especially for high density immiscible fluids. Gravity force nevertheless a predominant controlling factor in the migration of highly dense mercury, is counteracted by not less trivial capillary force which was 1.22x104 times higher than gravity force. The capillary forces thus surmount the gravity forces and cause entrapment of mercury in the soil pores even in homogeneous porous medium system. Bond number (Bond number relates gravity and capillary forces) for mercury-water system was found to 2.5 times higher than PCE-water systems. Large density differences between mercury and water lead to high Bond number and thus less residual saturation. Capillary number (Capillary number relates viscous and capillary forces) was found to be less for mercury-water systems. Literature review unveils that low Capillary number does not influence non wetting residual saturation. But for high density mercury with natural infiltration, even low Capillary number influences residual saturation. With the alarming increase in number of mercury spill sites, results of this study showed a better understanding of the capillary entrapment phenomena and the extent of influence of each predominant force during displacement of highly dense mercury. The fundamental inputs to NAPL entrapment models were generated in this study for mercury for the first time. This data will be used to assess the distribution of mercury in contaminated sites and design suitable remedial alternatives.

Water Dynamics and Dewetting Transitions in the Small Mechanosensitive Channel MscS

PubMed Central

Anishkin, Andriy; Sukharev, Sergei

2004-01-01

The dynamics of confined water in capillaries and nanotubes suggests that gating of ion channels may involve not only changes of the pore geometry, but also transitions between water-filled and empty states in certain locations. The recently solved heptameric structure of the small mechanosensitive channel of Escherichia coli, MscS, has revealed a relatively wide (7–15 Å) yet highly hydrophobic transmembrane pore. Continuum estimations based on the properties of pore surface suggest low conductance and a thermodynamic possibility of dewetting. To test the predictions we performed molecular dynamics simulations of MscS filled with flexible TIP3P water. Irrespective to the initial conditions, several independent 6-ns simulations converged to the same stable state with the pore water-filled in the wider part, but predominantly empty in the narrow hydrophobic part, displaying intermittent vapor-liquid transitions. The polar gain-of-function substitution L109S in the constriction resulted in a stable hydration of the entire pore. Steered passages of Cl− ions through the narrow part of the pore consistently produced partial ion dehydration and required a force of 200–400 pN to overcome an estimated barrier of 10–20 kcal/mole, implying negligibly low conductance. We conclude that the crystal structure of MscS does not represent an open state. We infer that MscS gate, which is similar to that of the nicotinic ACh receptor, involves a vapor-lock mechanism where limited changes of geometry or surface polarity can locally switch the regime between water-filled (conducting) and empty (nonconducting) states. PMID:15111405

Directional, passive liquid transport: the Texas horned lizard as a model for a biomimetic ‘liquid diode’

PubMed Central

Comanns, Philipp; Buchberger, Gerda; Buchsbaum, Andreas; Baumgartner, Richard; Kogler, Alexander; Bauer, Siegfried; Baumgartner, Werner

2015-01-01

Moisture-harvesting lizards such as the Texas horned lizard (Iguanidae: Phrynosoma cornutum) live in arid regions. Special skin adaptations enable them to access water sources such as moist sand and dew: their skin is capable of collecting and transporting water directionally by means of a capillary system between the scales. This fluid transport is passive, i.e. requires no external energy, and directs water preferentially towards the lizard's snout. We show that this phenomenon is based on geometric principles, namely on a periodic pattern of interconnected half-open capillary channels that narrow and widen. Following a biomimetic approach, we used these principles to develop a technical prototype design. Building upon the Young–Laplace equation, we derived a theoretical model for the local behaviour of the liquid in such capillaries. We present a global model for the penetration velocity validated by experimental data. Artificial surfaces designed in accordance with this model prevent liquid flow in one direction while sustaining it in the other. Such passive directional liquid transport could lead to process improvements and reduction of resources in many technical applications. PMID:26202685

[Relevance of contrast ultrasound with microbubbles in vascular medecine].

PubMed

Erdmann, Andreas; Ney, Barbara; Alatri, Adriano; Calanca, Luca; Mazzolai, Lucia

2016-12-07

Application of ultrasound contrast media has become a standard in diagnostic imaging in cardiology and in the characterization of focal lesions in multiple organs, especially of the liver. In the past years there was a growing body of evidence for their usefulness in vascular medicine. The development of contrast media, microbubbles with a stabilizing envelope and filled with gaz, small enough to pass through pulmonary capillaries made real-time imaging of organ perfusion possible. Ultrasound contrast media are rapidly eliminated by exhalation and can safely be administered to patients with renal failure. The objective of this review is to describe the basic principles of ultrasound contrast imaging and to inform about vascular applications of contrast ultrasound.

The fabrication of integrated carbon pipes with sub-micron diameters

NASA Astrophysics Data System (ADS)

Kim, B. M.; Murray, T.; Bau, H. H.

2005-08-01

A method for fabricating integrated carbon pipes (nanopipettes) of sub-micron diameters and tens of microns in length is demonstrated. The carbon pipes are formed from a template consisting of the tip of a pulled alumino-silicate glass capillary coated with carbon deposited from a vapour phase. This method renders carbon nanopipettes without the need for ex situ assembly and facilitates parallel production of multiple carbon-pipe devices. An electric-field-driven transfer of ions in a KCl solution through the integrated carbon pipes exhibits nonlinear current-voltage (I-V) curves, markedly different from the Ohmic I-V curves observed in glass pipettes under similar conditions. The filling of the nanopipette with fluorescent suspension is also demonstrated.

Adsorption of nicotine and tar from the mainstream smoke of cigarettes by oxidized carbon nanotubes

NASA Astrophysics Data System (ADS)

Chen, Zhigang; Zhang, Lisha; Tang, Yiwen; Jia, Zhijie

2006-02-01

The adsorption of nicotine and tar from the mainstream smoke (MS) by the filter tips filled respectively with oxidized carbon nanotubes (O-CNTs), activated carbon and zeolite (NaY) has been investigated. O-CNTs show exceptional removal efficiency and their adsorption mechanism is investigated. Capillary condensation of some ingredients from MS in the inner hole of O-CNTs is observed and may be the primary reason for their superior removal efficiency. The effect of O-CNTs mass on the removal efficiencies is also studied and the results show that about 20-30 mg O-CNTs per cigarette can effectively remove most of nicotine and tar.

Method of forming and assembly of parts

DOEpatents

Ripley, Edward B.

2010-12-28

A method of assembling two or more parts together that may be metal, ceramic, metal and ceramic parts, or parts that have different CTE. Individual parts are formed and sintered from particles that leave a network of interconnecting porosity in each sintered part. The separate parts are assembled together and then a fill material is infiltrated into the assembled, sintered parts using a method such as capillary action, gravity, and/or pressure. The assembly is then cured to yield a bonded and fully or near-fully dense part that has the desired physical and mechanical properties for the part's intended purpose. Structural strength may be added to the parts by the inclusion of fibrous materials.

[The use of white and yellow turpentine baths with diabetic patients].

PubMed

Davydova, O B; Turova, E A; Golovach, A V

1998-01-01

In patients with insulin-dependent diabetes mellitus while and yellow turpentine baths produced a positive effect on carbohydrate metabolism. White baths were more effective in respect to lipid metabolism, blood viscosity, produced a good effect on plasmic hemocoagulation factors. Both while and yellow turpentine baths were beneficial for capillary blood flow: initially high distal blood flow in patients with prevailing distal polyneuropathy decreased while in patients with macroangiopathy initially subnormal blood flow increased. Both white and yellow turpentine baths promoted better pulse blood filling of the lower limbs and weaker peripheral resistance of large vessels. In patients with non-insulin-dependent diabetes mellitus white and yellow turpentine baths contributed to normalization of carbohydrate metabolism. Yellow baths were more effective in lowering lipids. White baths induced inhibition of platelet aggregation but had no effect on coagulation, yellow baths promoted a reduction of fibrinogen but had no effect on platelet aggregation. Yellow baths produced more pronounced effect than white ones on blood viscosity and microcirculation. Both yellow and white baths stimulated pulse blood filling, corrected peripheral resistance of large and small vessels of the lower limbs.

Investigation of Episodic Flow from Unsaturated Porous Media into a Macropore

DOE Office of Scientific and Technical Information (OSTI.GOV)

R. K. Podgorney; J. P. Fairley

Th e recent literature contains numerous observations of episodic or intermittent fl ow in unsaturated flow systems under both constant fl ux and ponded boundary conditions. Flow systems composed of a heterogeneous porous media, as well as discrete fracture networks, have been cited as examples of systems that can exhibit episodic fl ow. Episodic outfl ow events are significant because relatively large volumes of water can move rapidly through an unsaturated system, carrying water and contaminants to depth greatly ahead of a wetting front predicted by a one-dimensional, gravity-driven diff usive infiltration model. In this study, we model the behaviormore » of water flow through a sand column underlain by an impermeable-walled macropore. Relative permeability and capillary pressure relationships were developed that capture the complex interrelationships between the macropore and the overlying porous media that control fl ow out of the system. The potential for episodic flow is assessed and compared to results of conventional modeling approaches and experimental data from the literature. Model results using coupled matrix–macropore relative permeability and capillary pressure relationships capture the behavior observed in laboratory experiments remarkably well, while simulations using conventional relative permeability and capillary pressure functions fail to capture some of the observed fl ow dynamics. Capturing the rapid downward movement of water suggests that the matrix-macropore capillary pressure and relative permeability functions developed have the potential to improve descriptions of fl ow and transport processes in heterogeneous, variably saturated media.« less

On-line coupling of immobilized cytochrome P450 microreactor and capillary electrophoresis: A promising tool for drug development.

PubMed

Schejbal, Jan; Řemínek, Roman; Zeman, Lukáš; Mádr, Aleš; Glatz, Zdeněk

2016-03-11

In this work, the combination of an immobilized enzyme microreactor (IMER) based on the clinically important isoform cytochrome P450 2C9 (CYP2C9) with capillary electrophoresis (CE) is presented. The CYP2C9 was attached to magnetic SiMAG-carboxyl microparticles using the carbodiimide method. The formation of an IMER in the inlet part of the separation capillary was ensured by two permanent magnets fixed in a cassette from the CE apparatus in the repulsive arrangement. The resulting on-line system provides an integration of enzyme reaction mixing and incubation, reaction products separation, detection and quantification into a single fully automated procedure with the possibility of repetitive use of the enzyme and minuscule amounts of reactant consumption. The on-line kinetic and inhibition studies of CYP2C9's reaction with diclofenac as a model substrate and sulfaphenazole as a model inhibitor were conducted in order to demonstrate its practical applicability. Values of the apparent Michalis-Menten constant, apparent maximum reaction velocity, Hill coefficient, apparent inhibition constant and half-maximal inhibition concentration were determined on the basis of the calculation of the effective substrate and inhibitor concentrations inside the capillary IMER using a model described by the Hagen-Poisseulle law and a novel enhanced model that reflects the influence of the reactants' diffusion during the injection process. Copyright © 2016 Elsevier B.V. All rights reserved.

P-gp Protein Expression and Transport Activity in Rodent Seizure Models and Human Epilepsy.

PubMed

Hartz, Anika M S; Pekcec, Anton; Soldner, Emma L B; Zhong, Yu; Schlichtiger, Juli; Bauer, Bjoern

2017-04-03

A cure for epilepsy is currently not available, and seizure genesis, seizure recurrence, and resistance to antiseizure drugs remain serious clinical problems. Studies show that the blood-brain barrier is altered in animal models of epilepsy and in epileptic patients. In this regard, seizures increase expression of blood-brain barrier efflux transporters such as P-glycoprotein (P-gp), which is thought to reduce brain uptake of antiseizure drugs, and thus, contribute to antiseizure drug resistance. The goal of the current study was to assess the viability of combining in vivo and ex vivo preparations of isolated brain capillaries from animal models of seizures and epilepsy as well as from patients with epilepsy to study P-gp at the blood-brain barrier. Exposing isolated rat brain capillaries to glutamate ex vivo upregulated P-gp expression to levels that were similar to those in capillaries isolated from rats that had status epilepticus or chronic epilepsy. Moreover, the fold-increase in P-gp protein expression seen in animal models is consistent with the fold-increase in P-gp observed in human brain capillaries isolated from patients with epilepsy compared to age-matched control individuals. Overall, the in vivo/ex vivo approach presented here allows detailed analysis of the mechanisms underlying seizure-induced changes of P-gp expression and transport activity at the blood-brain barrier. This approach can be extended to other blood-brain barrier proteins that might contribute to drug-resistant epilepsy or other CNS disorders as well.

Development of a design space and predictive statistical model for capsule filling of low-fill-weight inhalation products.

PubMed

Faulhammer, E; Llusa, M; Wahl, P R; Paudel, A; Lawrence, S; Biserni, S; Calzolari, V; Khinast, J G

2016-01-01

The objectives of this study were to develop a predictive statistical model for low-fill-weight capsule filling of inhalation products with dosator nozzles via the quality by design (QbD) approach and based on that to create refined models that include quadratic terms for significant parameters. Various controllable process parameters and uncontrolled material attributes of 12 powders were initially screened using a linear model with partial least square (PLS) regression to determine their effect on the critical quality attributes (CQA; fill weight and weight variability). After identifying critical material attributes (CMAs) and critical process parameters (CPPs) that influenced the CQA, model refinement was performed to study if interactions or quadratic terms influence the model. Based on the assessment of the effects of the CPPs and CMAs on fill weight and weight variability for low-fill-weight inhalation products, we developed an excellent linear predictive model for fill weight (R(2 )= 0.96, Q(2 )= 0.96 for powders with good flow properties and R(2 )= 0.94, Q(2 )= 0.93 for cohesive powders) and a model that provides a good approximation of the fill weight variability for each powder group. We validated the model, established a design space for the performance of different types of inhalation grade lactose on low-fill weight capsule filling and successfully used the CMAs and CPPs to predict fill weight of powders that were not included in the development set.

Enantioresolution in electrokinetic chromatography-complete filling technique using sulfated gamma-cyclodextrin. Software-free topological anticipation.

PubMed

Escuder-Gilabert, Laura; Martín-Biosca, Yolanda; Medina-Hernández, María José; Sagrado, Salvador

2016-10-07

Few papers have tried to predict the resolution ability of chiral selectors in capillary electrophoresis for the separation of the enantiomers of chiral compounds. In a previous work, we have used molecular information available on-line to establish enantioresolution levels of basic compounds using highly sulfated β-CD (HS-β-CD) as chiral selector in electrokinetic chromatography-complete filling technique (EKC-CFT). The present study is a continuation of this previous work, introducing some novelties. In this work, the ability of sulfated γ-cyclodextrin (S-γ-CD) as chiral selector in EKC-CFT is modelled for the first time. Thirty-three structurally unrelated cationic and neutral compounds (drugs and pesticides) are studied. Categorical enantioresolution levels (RsC, 0 or 1) are assigned from experimental enantioresolution values obtained at different S-γ-CD concentrations. Novel topological parameters connected to the chiral carbon (C * -parameters) are introduced. Four C * -parameters and a topological parameter of the whole molecule (aromatic atom count) are the most important variables according to a discriminant partial least squares-variable selection process. It suggests the preponderance of the topology adjacent to the chiral carbon to anticipate the RsC levels. A software-free anticipation protocol for new molecules is proposed. Over the current set of molecules evaluated, 100% of correct anticipations (resolved and non-resolved compounds) are obtained, while anticipation of some compounds remains undetermined. A criterion is introduced to alert on compounds which should not be anticipated. Copyright © 2016 Elsevier B.V. All rights reserved.

Prototype of low thermal expansion materials: fabrication of mesoporous silica/polymer composites with densely filled polymer inside mesopore space.

PubMed

Kiba, Shosuke; Suzuki, Norihiro; Okawauchi, Yoshinori; Yamauchi, Yusuke

2010-09-03

A prototype of novel low thermal expansion materials using mesoporous silica particles is demonstrated. Mesoporous silica/polymer composites with densely filled polymer inside the mesopore space are fabricated by mechanically mixing both organically modified mesoporous silica and epoxy polymer. The mesopores are easily penetrated by polymers as a result of the capillary force during the mechanical composite processing. Furthermore, we propose a new model of polymer mobility restriction using mesoporous silica with a large pore space. The robust inorganic frameworks covering the polymer effectively restrict the polymer mobility against thermal energy. As a result, the degree of total thermal expansion of the composites is drastically decreased. From the mass-normalized thermal mechanical analysis (TMA) charts of various composites with different amounts of mesoporous silica particles, it is observed that the coefficient of thermal expansion (CTE) values gradually increase with an increase of the polymer amount outside the mesopores. It is proven that the CTE values in the range over the glass-transition temperatures (T(g)) are perfectly proportional to the outside polymer amounts. Importantly, the Y-intercept of the relation equation obtained by a least-square method is the CTE value and is almost zero. This means that thermal expansion does not occur if no polymers are outside the mesopores. Through such a quantative discussion, we clarify that only the outside polymer affects the thermal expansion of the composites, that is, the embedded polymers inside the mesopores do not expand at all during the thermal treatment.

Enabling two-phase microfluidic thermal transport systems using a novel thermal-flux degassing and fluid charging approach

NASA Astrophysics Data System (ADS)

Singh Dhillon, Navdeep; Pisano, Albert P.

2014-03-01

A novel two-port thermal-flux method has been proposed and demonstrated for degassing and charging two-phase microfluidic thermal transport systems with a degassed working fluid. In microscale heat pipes and loop heat pipes (mLHPs), small device volumes and large capillary forces associated with smaller feature sizes render conventional vacuum pump-based degassing methods quite impractical. Instead, we employ a thermally generated pressure differential to purge non-condensable gases from these devices before charging them with a degassed working fluid in a two-step process. Based on the results of preliminary experiments studying the effectiveness and reliability of three different high temperature-compatible device packaging approaches, an optimized compression packaging technique was developed to degas and charge a mLHP device using the thermal-flux method. An induction heating-based noninvasive hermetic sealing approach for permanently sealing the degassed and charged mLHP devices has also been proposed. To demonstrate the efficacy of this approach, induction heating experiments were performed to noninvasively seal 1 mm square silicon fill-hole samples with donut-shaped solder preforms. The results show that the minimum hole sealing induction heating time is heat flux limited and can be estimated using a lumped capacitance thermal model. However, further continued heating of the solder uncovers the hole due to surface tension-induced contact line dynamics of the molten solder. It was found that an optimum mass of the solder preform is required to ensure a wide enough induction-heating time window for successful sealing of a fill-hole.

Myocardial serotonin exchange: negligible uptake by capillary endothelium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moffett, T.C.; Chan, I.S.; Bassingthwaighte, J.B.

1988-03-01

The extraction of serotonin from the blood during transorgan passage through the heart was studied using Langendorff-perfused rabbit hearts. Outflow dilution curves of /sup 131/I- or /sup 125/I-labeled albumin, (/sup 14/C)sucrose, and (3H)serotonin injected simultaneously into the inflow were fitted with an axially distributed blood-tissue exchange model to examine the extraction process. The model fits of the albumin and sucrose outflow dilution curves were used to define flow heterogeneity, intravascular dispersion, capillary permeability, and the volume of the interstitial space, which reduced the degrees of freedom in fitting the model to the serotonin curves. Serotonin extractions, measured against albumin, duringmore » single transcapillary passage, ranged from 24 to 64%. The ratio of the capillary permeability-surface area products for serotonin and sucrose, based on the maximum instantaneous extraction, was 1.37 +/- 0.2 (n = 18), very close to the predicted value of 1.39, the ratio of free diffusion coefficients calculated from the molecular weights. This result shows that the observed uptake of serotonin can be accounted for solely on the basis of diffusion between endothelial cells into the interstitial space. Thus it appears that the permeability of the luminal surface of the endothelial cell is negligible in comparison to diffusion through the clefts between endothelial cells. In 18 sets of dilution curves, with and without receptor and transport blockers or competitors (ketanserin, desipramine, imipramine, serotonin), the extractions and estimates of the capillary permeability-surface area product were not reduced, nor were the volumes of distribution. The apparent absence of transporters and receptors in rabbit myocardial capillary endothelium contrasts with their known abundance in the pulmonary vasculature.« less

Interfacial spreading effects on one-dimensional organic liquid imbibition in water-wetted porous media

NASA Astrophysics Data System (ADS)

McBride, J. F.; Simmons, C. S.; Cary, J. W.

1992-10-01

The spreading coefficient, Csp, determines whether an organic immiscible liquid, OIL, will form a lens ( Csp < 0) or will spread spontaneously ( Csp > 0) on a water surface. An OIL that forms a lens does not perfectly wet the water surface and therefore has a contact angle greater than 0°. The one-dimensional rate at which an OIL spreads spontaneously on a water surface is proportional to the square root of Csp. Of the OIL's that pose a contaminant threat to the subsurface, the majority has a non-zero Csp. To test the influence of such interfacial spreading phenomena on OIL infiltration in a pristine vadose zone, upward OIL and water imbibition infiltration experiments were performed in glass-bead columns, moistened with water, by using OIL's with different Csp. An analytical model for saturated liquid front rise was used to inversely estimate the effective capillary pressure head at the front and the average liquid conductivity. A nonspreading OIL ( Csp ≪ 0) exhibited a reduced capillary pressure head in the water-wetted glass beads. A spontaneously spreading OIL ( Csp ≫0) manifested an enhanced capillary pressure head. Reduced capillary pressure head was associated with an increase in average conductivity, and enhanced capillary pressure head was associated with a decrease in average conductivity when compared to the average water conductivity during water imbibition. The employed experimental method and mathematical analysis of dynamic flow, subject to interfacial spreading phenomena, are practical for quantifying parameters for use in sharp-front OIL infiltration models, but more research is needed to determine how to incorporate the spreading coefficient in numerical multiphase flow models.

Moist air state above counterflow wet-cooling tower fill based on Merkel, generalised Merkel and Klimanek & Białecky models

NASA Astrophysics Data System (ADS)

Hyhlík, Tomáš

2017-09-01

The article deals with an evaluation of moist air state above counterflow wet-cooling tower fill. The results based on Klimanek & Białecky model are compared with results of Merkel model and generalised Merkel model. Based on the numerical simulation it is shown that temperature is predicted correctly by using generalised Merkel model in the case of saturated or super-saturated air above the fill, but the temperature is underpredicted in the case of unsaturated moist air above the fill. The classical Merkel model always under predicts temperature above the fill. The density of moist air above the fill, which is calculated using generalised Merkel model, is strongly over predicted in the case of unsaturated moist air above the fill.

A microvascular compartment model validated using 11C-methylglucose liver PET in pigs

NASA Astrophysics Data System (ADS)

Munk, Ole L.; Keiding, Susanne; Baker, Charles; Bass, Ludvik

2018-01-01

The standard compartment model (CM) is widely used to analyse dynamic PET data. The CM is fitted to time-activity curves to estimate rate constants that describe the transport of a tracer between well-mixed compartments. The aim of this study was to develop and validate a more realistic microvascular compartment model (MCM) that includes capillary tracer concentration gradients, backflux from cells into the perfused capillaries and multiple re-uptakes during the passage through a capillary. The MCM incorporates only parameters with clear physiological meaning, it is easy to implement, and it does not require numerical solution. We compared the MCM and CM for the analysis of 3 min dynamic PET data of pig livers (N  =  5) following injection of 11C-methylglucose. During PET scans, the tracer concentrations in blood were measured in the abdominal aorta, portal vein and liver vein by manual sampling. We found that the MCM outperformed the CM and that dynamic PET data include information which cannot be extracted using standard CM. The MCM fitted dynamic PET data better than the CM (Akaike values were 46  ±  4 for best MCM fits, and 82  ±  8 for best CM fits; mean  ±  standard deviation) and extracted physiologically reasonable parameter estimates such as blood perfusion that were in agreement with independent measurements. The difference between model-independent perfusion estimates and the best MCM perfusion estimates was  -0.01  ±  0.05 ml/ml/min, whereas the difference was 0.30  ±  0.13 ml/ml/min using the CM. In addition, the MCM predicted the time course of concentrations in the liver vein, a prediction fundamentally unobtainable using the CM as it does not return tracer backflux from cells to capillary blood. The results demonstrate the benefit of using models that include more physiology and that models including concentration gradients should be preferred when analysing the blood-cell exchange of any tracer in any capillary bed.

Computational Analyses of Complex Flows with Chemical Reactions

NASA Astrophysics Data System (ADS)

Bae, Kang-Sik

The heat and mass transfer phenomena in micro-scale for the mass transfer phenomena on drug in cylindrical matrix system, the simulation of oxygen/drug diffusion in a three dimensional capillary network, and a reduced chemical kinetic modeling of gas turbine combustion for Jet propellant-10 have been studied numerically. For the numerical analysis of the mass transfer phenomena on drug in cylindrical matrix system, the governing equations are derived from the cylindrical matrix systems, Krogh cylinder model, which modeling system is comprised of a capillary to a surrounding cylinder tissue along with the arterial distance to veins. ADI (Alternative Direction Implicit) scheme and Thomas algorithm are applied to solve the nonlinear partial differential equations (PDEs). This study shows that the important factors which have an effect on the drug penetration depth to the tissue are the mass diffusivity and the consumption of relevant species during the time allowed for diffusion to the brain tissue. Also, a computational fluid dynamics (CFD) model has been developed to simulate the blood flow and oxygen/drug diffusion in a three dimensional capillary network, which are satisfied in the physiological range of a typical capillary. A three dimensional geometry has been constructed to replicate the one studied by Secomb et al. (2000), and the computational framework features a non-Newtonian viscosity model for blood, the oxygen transport model including in oxygen-hemoglobin dissociation and wall flux due to tissue absorption, as well as an ability to study the diffusion of drugs and other materials in the capillary streams. Finally, a chemical kinetic mechanism of JP-10 has been compiled and validated for a wide range of combustion regimes, covering pressures of 1atm to 40atm with temperature ranges of 1,200 K--1,700 K, which is being studied as a possible Jet propellant for the Pulse Detonation Engine (PDE) and other high-speed flight applications such as hypersonic missiles. The comprehensive skeletal mechanism consists of 58 species and 315 reactions including in CPD, Benzene formation process by the theory for polycyclic aromatic hydrocarbons (PAH) and soot formation process on the constant volume combustor, premixed flame characteristics.

The impact of capillary backpressure on spontaneous counter-current imbibition in porous media

NASA Astrophysics Data System (ADS)

Foley, Amir Y.; Nooruddin, Hasan A.; Blunt, Martin J.

2017-09-01

We investigate the impact of capillary backpressure on spontaneous counter-current imbibition. For such displacements in strongly water-wet systems, the non-wetting phase is forced out through the inlet boundary as the wetting phase imbibes into the rock, creating a finite capillary backpressure. Under the assumption that capillary backpressure depends on the water saturation applied at the inlet boundary of the porous medium, its impact is determined using the continuum modelling approach by varying the imposed inlet saturation in the analytical solution. We present analytical solutions for the one-dimensional incompressible horizontal displacement of a non-wetting phase by a wetting phase in a porous medium. There exists an inlet saturation value above which any change in capillary backpressure has a negligible impact on the solutions. Above this threshold value, imbibition rates and front positions are largely invariant. A method for identifying this inlet saturation is proposed using an analytical procedure and we explore how varying multiphase flow properties affects the analytical solutions and this threshold saturation. We show the value of this analytical approach through the analysis of previously published experimental data.

Harvesting liquid from unsaturated vapor - nanoflows induced by capillary condensation

NASA Astrophysics Data System (ADS)

Vincent, Olivier; Marguet, Bastien; Stroock, Abraham

2016-11-01

A vapor, even subsaturated, can spontaneously form liquid in nanoscale spaces. This process, known as capillary condensation, plays a fundamental role in various contexts, such as the formation of clouds or the dynamics of hydrocarbons in the geological subsurface. However, large uncertainties remain on the thermodynamics and fluid mechanics of the phenomenon, due to experimental challenges as well as outstanding questions about the validity of macroscale physics at the nanometer scale. We studied experimentally the spatio-temporal dynamics of water condensation in a model nanoporous medium (pore radius 2 nm), taking advantage of the color change of the material upon hydration. We found that at low relative humidities (< 60 % RH), capillary condensation progressed in a diffusive fashion, while it occurred through a well-defined capillary-viscous imbibition front at > 60 % RH, driven by a balance between the pore capillary pressure and the condensation stress given by Kelvin equation. Further analyzing the imbibition dynamics as a function of saturation allowed us to extract detailed information about the physics of nano-confined fluids. Our results suggest excellent extension of macroscale fluid dynamics and thermodynamics even in pores 10 molecules in diameter.

Modeling of FMISO [F18] nanoparticle PET tracer in normal-cancerous tissue based on real clinical image.

PubMed

Asgari, Hanie; Soltani, M; Sefidgar, Mostafa

2018-07-01

Hypoxia as one of the principal properties of tumor cells is a reaction to the deprivation of oxygen. The location of tumor cells could be identified by assessment of oxygen and nutrient level in human body. Positron emission tomography (PET) is a well-known non-invasive method that is able to measure hypoxia based on the FMISO (Fluoromisonidazole) tracer dynamic. This paper aims to study the PET tracer concentration through convection-diffusion-reaction equations in a real human capillary-like network. A non-uniform oxygen pressure along the capillary path and convection mechanism for FMISO transport are taken into account to accurately model the characteristics of the tracer. To this end, a multi-scale model consists of laminar blood flow through the capillary network, interstitial pressure, oxygen pressure, FMISO diffusion and FMISO convection transport in the extravascular region is developed. The present model considers both normal and tumor tissue regions in computational domain. The accuracy of numerical model is verified with the experimental results available in the literature. The convection and diffusion types of transport mechanism are employed in order to calculate the concentration of FMISO in the normal and tumor sub-domain. The influences of intravascular oxygen pressure, FMISO transport mechanisms, capillary density and different types of tissue on the FMISO concentration have been investigated. According to result (Table 4) the convection mechanism of FMISO molecules transportation is negligible, but it causes more accuracy of the proposed model. The approach of present study can be employed in order to investigate the effects of various parameters, such as tumor shape, on the dynamic behavior of different PET tracers, such as FDG, can be extended to different case study problems, such as drug delivery. Copyright © 2018 Elsevier Inc. All rights reserved.

The Pathway for Oxygen: Tutorial Modelling on Oxygen Transport from Air to Mitochondrion: The Pathway for Oxygen.

PubMed

Bassingthwaighte, James B; Raymond, Gary M; Dash, Ranjan K; Beard, Daniel A; Nolan, Margaret

2016-01-01

The 'Pathway for Oxygen' is captured in a set of models describing quantitative relationships between fluxes and driving forces for the flux of oxygen from the external air source to the mitochondrial sink at cytochrome oxidase. The intervening processes involve convection, membrane permeation, diffusion of free and heme-bound O2 and enzymatic reactions. While this system's basic elements are simple: ventilation, alveolar gas exchange with blood, circulation of the blood, perfusion of an organ, uptake by tissue, and consumption by chemical reaction, integration of these pieces quickly becomes complex. This complexity led us to construct a tutorial on the ideas and principles; these first PathwayO2 models are simple but quantitative and cover: (1) a 'one-alveolus lung' with airway resistance, lung volume compliance, (2) bidirectional transport of solute gasses like O2 and CO2, (3) gas exchange between alveolar air and lung capillary blood, (4) gas solubility in blood, and circulation of blood through the capillary syncytium and back to the lung, and (5) blood-tissue gas exchange in capillaries. These open-source models are at Physiome.org and provide background for the many respiratory models there.

The Pathway for Oxygen: Tutorial Modelling on Oxygen Transport from Air to Mitochondrion

PubMed Central

Bassingthwaighte, James B.; Raymond, Gary M.; Dash, Ranjan K.; Beard, Daniel A.; Nolan, Margaret

2016-01-01

The ‘Pathway for Oxygen’ is captured in a set of models describing quantitative relationships between fluxes and driving forces for the flux of oxygen from the external air source to the mitochondrial sink at cytochrome oxidase. The intervening processes involve convection, membrane permeation, diffusion of free and heme-bound O2 and enzymatic reactions. While this system’s basic elements are simple: ventilation, alveolar gas exchange with blood, circulation of the blood, perfusion of an organ, uptake by tissue, and consumption by chemical reaction, integration of these pieces quickly becomes complex. This complexity led us to construct a tutorial on the ideas and principles; these first PathwayO2 models are simple but quantitative and cover: 1) a ‘one-alveolus lung’ with airway resistance, lung volume compliance, 2) bidirectional transport of solute gasses like O2 and CO2, 3) gas exchange between alveolar air and lung capillary blood, 4) gas solubility in blood, and circulation of blood through the capillary syncytium and back to the lung, and 5) blood-tissue gas exchange in capillaries. These open-source models are at Physiome.org and provide background for the many respiratory models there. PMID:26782201

Fast plasma discharge capillary design as a high power throughput soft x-ray emission source.

PubMed

Wyndham, E S; Favre, M; Valdivia, M P; Valenzuela, J C; Chuaqui, H; Bhuyan, H

2010-09-01

We present the experimental details and results from a low energy but high repetition rate compact plasma capillary source for extreme ultraviolet and soft x-ray research and applications. Two lengths of capillary are mounted in two versions of a closely related design. The discharge operates in 1.6 and 3.2 mm inner diameter alumina capillaries of lengths 21 and 36 mm. The use of water both as dielectric and as coolant simplifies the compact low inductance design with nanosecond discharge periods. The stored electrical energy of the discharge is approximately 0.5 J and is provided by directly charging the capacitor plates from an inexpensive insulated-gate bipolar transistor in 1 μs or less. We present characteristic argon spectra from plasma between 30 and 300 Å as well as temporally resolved x-ray energy fluence in discrete bands on axis. The spectra also allow the level of ablated wall material to be gauged and associated with useful capillary lifetime according to the chosen configuration and energy storage. The connection between the electron beams associated with the transient hollow cathode mechanism, soft x-ray output, capillary geometry, and capillary lifetime is reported. The role of these e-beams and the plasma as measured on-axis is discussed. The relation of the electron temperature and the ionization stages observed is discussed in the context of some model results of ionization in a non-Maxwellian plasma.

A diffusivity model for predicting VOC diffusion in porous building materials based on fractal theory.

PubMed

Liu, Yanfeng; Zhou, Xiaojun; Wang, Dengjia; Song, Cong; Liu, Jiaping

2015-12-15

Most building materials are porous media, and the internal diffusion coefficients of such materials have an important influences on the emission characteristics of volatile organic compounds (VOCs). The pore structure of porous building materials has a significant impact on the diffusion coefficient. However, the complex structural characteristics bring great difficulties to the model development. The existing prediction models of the diffusion coefficient are flawed and need to be improved. Using scanning electron microscope (SEM) observations and mercury intrusion porosimetry (MIP) tests of typical porous building materials, this study developed a new diffusivity model: the multistage series-connection fractal capillary-bundle (MSFC) model. The model considers the variable-diameter capillaries formed by macropores connected in series as the main mass transfer paths, and the diameter distribution of the capillary bundles obeys a fractal power law in the cross section. In addition, the tortuosity of the macrocapillary segments with different diameters is obtained by the fractal theory. Mesopores serve as the connections between the macrocapillary segments rather than as the main mass transfer paths. The theoretical results obtained using the MSFC model yielded a highly accurate prediction of the diffusion coefficients and were in a good agreement with the VOC concentration measurements in the environmental test chamber. Copyright © 2015 Elsevier B.V. All rights reserved.

Infiltration of MHD liquid into a deformable porous material

NASA Astrophysics Data System (ADS)

Naseem, Anum; Mahmood, Asif; Siddique, J. I.; Zhao, Lifeng

2018-03-01

We analyze the capillary rise dynamics for magnetohydrodynamics (MHD) fluid flow through deformable porous material in the presence of gravity effects. The modeling is performed using mixture theory approach and mathematical manipulation yields a nonlinear free boundary problem. Due to the capillary rise action, the pressure gradient in the liquid generates a stress gradient that results in the deformation of porous substrate. The capillary rise process for MHD fluid slows down as compared to Newtonian fluid case. Numerical solutions are obtained using a method of lines approach. The graphical results are presented for important physical parameters, and comparison is presented with Newtonian fluid case.

Capillary fluctuations of surface steps: An atomistic simulation study for the model Cu(111) system

NASA Astrophysics Data System (ADS)

Freitas, Rodrigo; Frolov, Timofey; Asta, Mark

2017-10-01

Molecular dynamics (MD) simulations are employed to investigate the capillary fluctuations of steps on the surface of a model metal system. The fluctuation spectrum, characterized by the wave number (k ) dependence of the mean squared capillary-wave amplitudes and associated relaxation times, is calculated for 〈110 〉 and 〈112 〉 steps on the {111 } surface of elemental copper near the melting temperature of the classical potential model considered. Step stiffnesses are derived from the MD results, yielding values from the largest system sizes of (37 ±1 ) meV/A ˚ for the different line orientations, implying that the stiffness is isotropic within the statistical precision of the calculations. The fluctuation lifetimes are found to vary by approximately four orders of magnitude over the range of wave numbers investigated, displaying a k dependence consistent with kinetics governed by step-edge mediated diffusion. The values for step stiffness derived from these simulations are compared to step free energies for the same system and temperature obtained in a recent MD-based thermodynamic-integration (TI) study [Freitas, Frolov, and Asta, Phys. Rev. B 95, 155444 (2017), 10.1103/PhysRevB.95.155444]. Results from the capillary-fluctuation analysis and TI calculations yield statistically significant differences that are discussed within the framework of statistical-mechanical theories for configurational contributions to step free energies.

Electrochemistry and capillary condensation theory reveal the mechanism of corrosion in dense porous media.

PubMed

Stefanoni, Matteo; Angst, Ueli M; Elsener, Bernhard

2018-05-09

Corrosion in carbonated concrete is an example of corrosion in dense porous media of tremendous socio-economic and scientific relevance. The widespread research endeavors to develop novel, environmentally friendly cements raise questions regarding their ability to protect the embedded steel from corrosion. Here, we propose a fundamentally new approach to explain the scientific mechanism of corrosion kinetics in dense porous media. The main strength of our model lies in its simplicity and in combining the capillary condensation theory with electrochemistry. This reveals that capillary condensation in the pore structure defines the electrochemically active steel surface, whose variability upon changes in exposure relative humidity is accountable for the wide variability in measured corrosion rates. We performed experiments that quantify this effect and find good agreement with the theory. Our findings are essential to devise predictive models for the corrosion performance, needed to guarantee the safety and sustainability of traditional and future cements.

Blood Vessel Adaptation with Fluctuations in Capillary Flow Distribution

PubMed Central

Hu, Dan; Cai, David; Rangan, Aaditya V.

2012-01-01

Throughout the life of animals and human beings, blood vessel systems are continuously adapting their structures – the diameter of vessel lumina, the thickness of vessel walls, and the number of micro-vessels – to meet the changing metabolic demand of the tissue. The competition between an ever decreasing tendency of luminal diameters and an increasing stimulus from the wall shear stress plays a key role in the adaptation of luminal diameters. However, it has been shown in previous studies that the adaptation dynamics based only on these two effects is unstable. In this work, we propose a minimal adaptation model of vessel luminal diameters, in which we take into account the effects of metabolic flow regulation in addition to wall shear stresses and the decreasing tendency of luminal diameters. In particular, we study the role, in the adaptation process, of fluctuations in capillary flow distribution which is an important means of metabolic flow regulation. The fluctuation in the flow of a capillary group is idealized as a switch between two states, i.e., an open-state and a close-state. Using this model, we show that the adaptation of blood vessel system driven by wall shear stress can be efficiently stabilized when the open time ratio responds sensitively to capillary flows. As micro-vessel rarefaction is observed in our simulations with a uniformly decreased open time ratio of capillary flows, our results point to a possible origin of micro-vessel rarefaction, which is believed to induce hypertension. PMID:23029014

Enantiomeric separation of some demethylated analogues of clofibric acid by capillary zone electrophoresis and nano-liquid chromatography.

PubMed

Fantacuzzi, Marialuigia; Bettoni, Giancarlo; D'Orazio, Giovanni; Fanali, Salvatore

2006-03-01

The enantiomeric separation of some demethylated analogues of clofibric acid, namely 2-(6-chloro-benzothiazol-2-ylsulfanyl)-, 2-(6-methoxy-benzothiazol-2-ylsulfanyl)-, 2-(quinolin-2-yloxy)-, 2-(6-chloro-quinolin-2-yloxy)-, 2-(7-chloro-quinolin-4-yloxy)-propionic acid (compounds A-E, respectively), has been studied by CZE and nano-LC using for the first technique two beta-CD derivatives and vancomycin added to the BGE and vancomycin-modified silica particles for the second one, with the aim to find the optimum experimental conditions for the baseline resolution. The type and the concentration of the chiral selector added to the BGE, the buffer pH, the type of organic modifier and its concentration, the capillary temperature and the applied voltage played a very important role in the enantioresolution of the analysed compounds. The use of 6-monodeoxy-6-monoamino-beta-CD allowed to achieve baseline resolution of four of five clofibric acid derivatives in less than 10 min while heptakis-(2,3,6-tri-O-methyl)-beta-CD partially resolved the same compounds in their enantiomers. Employing vancomycin as the chiral selector in CZE, the counter-current partial filling method was chosen achieving baseline resolution of four analytes. All the studied compounds were enantioresolved employing a capillary column packed with vancomycin stationary phase by nano-LC, and the resolution was strongly influenced by the concentration of the organic modifier and by the pH of the mobile phase. The best results were achieved at pH 4.5 in presence of 60% of methanol (MeOH). However, longer analysis times were observed in the experiments carried out by nano-LC.

PARACENTRAL ACUTE MIDDLE MACULOPATHY IN A PERIVENULAR FERN-LIKE DISTRIBUTION WITH EN FACE OPTICAL COHERENCE TOMOGRAPHY.

PubMed

Garrity, Sean T; Tseng, Victoria L; Sarraf, David

2017-11-22

To report a case of central retinal vein occlusion resulting in a perivenular pattern of paracentral acute middle maculopathy lesions best identified with en face optical coherence tomography (OCT). Retrospective case report. Optos ultra-widefield fluorescein angiography, spectral domain OCT, en face OCT, and OCT angiography were performed. A 41-year-old man presented with decreased vision in the right eye for 2 weeks. Funduscopic examination of the affected right eye was notable for subtle retinal whitening in the macula, mild retinal venous dilation and tortuosity, and few scattered retinal dot and blot hemorrhages consistent with an acute central retinal vein occlusion. Widefield fluorescein angiography demonstrated delayed arterial and venous filling but no evidence of significant peripheral retinal vascular ischemia. En face OCT segmented at the inner nuclear layer illustrated a remarkable and precise perivenular distribution of fern-like paracentral acute middle maculopathy with periarterial sparing, whereas en face OCT segmented at the outer nuclear layer demonstrated florid cystoid macular edema. At 6-week follow-up, OCT demonstrated patchy areas of atrophic inner nuclear layer and spontaneous resolution of the cystoid macular edema. Optical coherence tomography angiography at the level of the deep capillary plexus illustrated remarkable flow reduction of the deep capillary plexus in mainly a perivenular distribution. The authors report a case of a central retinal vein occlusion with mild retinal findings associated with a remarkable perivenular pattern of paracentral acute middle maculopathy with en face OCT. Follow-up OCT angiography demonstrated significant flow reduction of the deep capillary plexus in a perivenular pattern. The perivenular pattern of paracentral acute middle maculopathy lesions with en face OCT can be an important finding suggestive of a central retinal vein occlusion.

A lattice Boltzmann investigation of steady-state fluid distribution, capillary pressure and relative permeability of a porous medium: Effects of fluid and geometrical properties

NASA Astrophysics Data System (ADS)

Li, Zi; Galindo-Torres, Sergio; Yan, Guanxi; Scheuermann, Alexander; Li, Ling

2018-06-01

Simulations of simultaneous steady-state two-phase flow in the capillary force-dominated regime were conducted using the state-of-the-art Shan-Chen multi-component lattice Boltzmann model (SCMC-LBM) based on two-dimensional porous media. We focused on analyzing the fluid distribution (i.e., WP fluid-solid, NP fluid-solid and fluid-fluid interfacial areas) as well as the capillary pressure versus saturation curve which was affected by fluid and geometrical properties (i.e., wettability, adhesive strength, pore size distribution and specific surface area). How these properties influenced the relative permeability versus saturation relation through apparent effective permeability and threshold pressure gradient was also explored. The SCMC-LBM simulations showed that, a thin WP fluid film formed around the solid surface due to the adhesive fluid-solid interaction, resulting in discrete WP fluid distributions and reduction of the WP fluid mobility. Also, the adhesive interaction provided another source of capillary pressure in addition to capillary force, which, however, did not affect the mobility of the NP fluid. The film fluid effect could be enhanced by large adhesive strength and fine pores in heterogeneous porous media. In the steady-state infiltration, not only the NP fluid but also the WP fluid were subjected to the capillary resistance. The capillary pressure effect could be alleviated by decreased wettability, large average pore radius and improved fluid connectivity in heterogeneous porous media. The present work based on the SCMC-LBM investigations elucidated the role of film fluid as well as capillary pressure in the two-phase flow system. The findings have implications for ways to improve the macroscopic flow equation based on balance of force for the steady-state infiltration.

Effect of hypothermic pulmonary artery flushing on capillary filtration coefficient.

PubMed

Andrade, R S; Wangensteen, O D; Jo, J K; Tsai, M Y; Bolman, R M

2000-07-27

We previously demonstrated that surfactant dilution and inhibition occur immediately after pulmonary artery flushing with hypothermic modified Euro-Collins solution. Consequently, we speculated that increased capillary permeability contributed to these surfactant changes. To test this hypothesis, we evaluated the effects of hypothermic pulmonary artery flushing on the pulmonary capillary filtration coefficient (Kfc), and additionally performed a biochemical analysis of surfactant. We used a murine isolated, perfused lung model to measure the pulmonary capillary filtration coefficient and hemodynamic parameters, to determine the wet to dry weight ratio, and to evaluate surfactant by biochemical analysis of lung lavage fluid. We defined three study groups. In group I (controls), we harvested lungs without hypothermic pulmonary artery flushing, and measured Kfc immediately. In group II (in situ flush), we harvested lungs after hypothermic pulmonary artery flushing with modified Euro-Collins solution, and then measured Kfc. Experiments in groups I and II were designed to evaluate persistent changes in Kfc after pulmonary artery flushing. In group III (ex vivo flush), we flushed lungs ex vivo to evaluate transient changes in Kfc during hypothermic pulmonary artery flushing. Groups I and II did not differ significantly in capillary filtration coefficient and hemodynamics. Group II showed significant alterations on biochemical surfactant analysis and a significant increase in wet-to-dry weight ratio, when compared with group I. In group III, we observed a significant transient increase in capillary filtration coefficient during pulmonary artery flushing. Hypothermic pulmonary artery flushing transiently increases the capillary filtration coefficient, leads to an increase in the wet to dry weight ratio, and induces biochemical surfactant changes. These findings could be explained by the effects of hypothermic modified Euro-Collins solution on pulmonary capillary permeability.

Intrinsic advantages of packed capillaries over narrow-bore columns in very high-pressure gradient liquid chromatography.

PubMed

Gritti, Fabrice; McDonald, Thomas; Gilar, Martin

2016-06-17

250μm×100mm fused silica glass capillaries were packed with 1.8μm high-strength silica (HSS) fully porous particles. They were prepared without bulky stainless steel endfittings and metal frits, which both generate significant sample dispersion. The isocratic efficiencies and gradient peak capacities of these prototype capillary columns were measured for small molecules (n-alkanophenones) using a home-made ultra-low dispersive micro-HPLC instrument. Their resolution power was compared to that of standard 2.1mm×100mm very high-pressure liquid chromatography (vHPLC) narrow-bore columns packed with the same particles. The results show that, for the same column efficiency (25000 plates) and gradient steepness (0.04min(-1)), the peak capacity of the 250μm i.d. capillary columns is systematically 15-20% higher than that of the 2.1mm i.d. narrow-bore columns. A validated model of gradient chromatography enabled one to predict accurately the observed peak capacities of the capillary columns for non-linear solvation strength retention behavior and under isothermal conditions. Thermodynamics applied to the eluent quantified the temperature difference for the thermal gradients in both capillary and narrow-bore columns. Experimental data revealed that the gradient peak capacity is more affected by viscous heating than the column efficiency. Unlike across 2.1mm i.d. columns, the changes in eluent composition across the 250μm i.d. columns during the gradient is rapidly relaxed by transverse dispersion. The combination of (1) the absence of viscous heating and (2) the high uniformity of the eluent composition across the diameter of capillary columns explains the intrinsic advantage of capillary over narrow-bore columns in gradient vHPLC. Copyright © 2016 Elsevier B.V. All rights reserved.

Lower capillary density but no difference in VEGF expression in obese vs. lean young skeletal muscle in humans.

PubMed

Gavin, Timothy P; Stallings, Howard W; Zwetsloot, Kevin A; Westerkamp, Lenna M; Ryan, Nicholas A; Moore, Rebecca A; Pofahl, Walter E; Hickner, Robert C

2005-01-01

Obesity is associated with lower skeletal muscle capillarization and lower insulin sensitivity. Vascular endothelial growth factor (VEGF) is important for the maintenance of the skeletal muscle capillaries. To investigate whether VEGF and VEGF receptor [kinase insert domain-containing receptor (KDR) and Flt-1] expression are lower with obesity, vastus lateralis muscle biopsies were obtained from eight obese and eight lean young sedentary men before and 2 h after a 1-h submaximal aerobic exercise bout for the measurement of VEGF, KDR, Flt-1, and skeletal muscle fiber and capillary characteristics. There were no differences in VEGF or VEGF receptor mRNA at rest between lean and obese muscle. Exercise increased VEGF (10-fold), KDR (3-fold), and Flt-1 (5-fold) mRNA independent of group. There were no differences in VEGF, KDR, or Flt-1 protein between groups. Compared with lean skeletal muscle, the number of capillary contacts per fiber was the same, but lower capillary density (CD), greater muscle cross sectional area, and lower capillary-to-fiber area ratio were observed in both type I and II fibers in obese muscle. Multiple linear regression revealed that 49% of the variance in insulin sensitivity (homeostasis model assessment) could be explained by percentage of body fat (35%) and maximal oxygen uptake per kilogram of fat-free mass (14%). Linear regression revealed significant relationships between maximal oxygen uptake and both CD and capillary-to-fiber perimeter exchange. Although differences may exist in CD and capillary-to-fiber area ratio between lean and obese skeletal muscle, the present results provide evidence that VEGF and VEGF receptor expression are not different between lean and obese muscle.

Vibration isolation technology: Sensitivity of selected classes of space experiments to residual accelerations

NASA Technical Reports Server (NTRS)

Alexander, J. Iwan D.; Zhang, Y. Q.; Adebiyi, Adebimpe

1989-01-01

Progress performed on each task is described. Order of magnitude analyses related to liquid zone sensitivity and thermo-capillary flow sensitivity are covered. Progress with numerical models of the sensitivity of isothermal liquid zones is described. Progress towards a numerical model of coupled buoyancy-driven and thermo-capillary convection experiments is also described. Interaction with NASA personnel is covered. Results to date are summarized and they are discussed in terms of the predicted space station acceleration environment. Work planned for the second year is also discussed.

Quantifying moisture transport in cementitious materials using neutron radiography

NASA Astrophysics Data System (ADS)

Lucero, Catherine L.

A portion of the concrete pavements in the US have recently been observed to have premature joint deterioration. This damage is caused in part by the ingress of fluids, like water, salt water, or deicing salts. The ingress of these fluids can damage concrete when they freeze and expand or can react with the cementitious matrix causing damage. To determine the quality of concrete for assessing potential service life it is often necessary to measure the rate of fluid ingress, or sorptivity. Neutron imaging is a powerful method for quantifying fluid penetration since it can describe where water has penetrated, how quickly it has penetrated and the volume of water in the concrete or mortar. Neutrons are sensitive to light atoms such as hydrogen and thus clearly detect water at high spatial and temporal resolution. It can be used to detect small changes in moisture content and is ideal for monitoring wetting and drying in mortar exposed to various fluids. This study aimed at developing a method to accurately estimate moisture content in mortar. The common practice is to image the material dry as a reference before exposing to fluid and normalizing subsequent images to the reference. The volume of water can then be computed using the Beer-Lambert law. This method can be limiting because it requires exact image alignment between the reference image and all subsequent images. A model of neutron attenuation in a multi-phase cementitious composite was developed to be used in cases where a reference image is not available. The attenuation coefficients for water, un-hydrated cement, and sand were directly calculated from the neutron images. The attenuation coefficient for the hydration products was then back-calculated. The model can estimate the degree of saturation in a mortar with known mixture proportions without using a reference image for calculation. Absorption in mortars exposed to various fluids (i.e., deionized water and calcium chloride solutions) were investigated. It has been found through this study that small pores, namely voids created by chemical shrinkage, gel pores, and capillary pores, ranging from 0.5 nm to 50 microm, fill quickly through capillary action. However, large entrapped and entrained air voids ranging from 0.05 to 1.25 mm remain empty during the initial filling process. In mortar exposed to calcium chloride solution, a decrease in sorptivity was observed due to an increase in viscosity and surface tension of the solution as proposed by Spragg et al 2011. This work however also noted a decrease in the rate of absorption due to a reaction between the salt and matrix which results in the filling of the pores in the concrete. The results from neutron imaging can help in the interpretation of standard absorption tests. ASTM C1585 test results can be further analyzed in several ways that could give an accurate indication of the durability of the concrete. Results can be reported in depth of penetration versus the square root of time rather than mm3 of fluid per mm2 of exposed surface area. Since a known fraction of pores are initially filling before reaching the edge of the sample, the actual depth of penetration can be calculated. This work is compared with an 'intrinsic sorptivity' that can be used to interpret mass measurements. Furthermore, the influence of shrinkage reducing admixtures (SRAs) on drying was studied. Neutron radiographs showed that systems saturated in water remain "wetter" than systems saturated in 5% SRA solution. The SRA in the system reduces the moisture diffusion coefficient due an increase in viscosity and decrease in surface tension. Neutron radiography provided spatial information of the drying front that cannot be achieved using other methods.

Steady displacement of long gas bubbles in channels and tubes filled by a Bingham fluid

NASA Astrophysics Data System (ADS)

Zamankhan, Parsa; Takayama, Shuichi; Grotberg, James B.

2018-01-01

Bingham fluids behave like solids below a von Mises stress threshold, the yield stress, while above it they behave like Newtonian fluids. They are characterized by a dimensionless parameter, Bingham number (Bn), which is the ratio of the yield stress to a characteristic viscous stress. In this study, the noninertial steady motion of a finite-size gas bubble in both a plane two-dimensional (2D) channel and an axisymmetric tube filled by a Bingham fluid has been studied numerically. The Bingham number, Bn, is in the range 0 ≤Bn ≤3 , where Bn =0 is the Newtonian case, while the capillary number, which is the ratio of a characteristic viscous force to the surface tension, has values Ca =0.05 ,0.10 , and 0.25. The volume of all axisymmetric and 2D bubbles has been chosen to be identical for all parameter choices and large enough for the bubbles to be long compared to the channel, tube height, and diameter. The Bingham fluid constitutive equation is approximated by a regularized equation. During the motion, the bubble interface is separated from the wall by a static liquid film. The film thickness scaled by the tube radius (axisymmetric) and half of the channel height (2D) is the dimensionless film thickness, h . The results show that increasing Bn initially leads to an increase in h ; however, the profile h versus Bn can be monotonic or nonmonotonic depending on Ca values and 2D or axisymmetric configurations. The yield stress also alters the shape of the front and rear of the bubble and suppresses the capillary waves at the rear of the bubble. The yield stress increases the magnitude of the wall shear stress and its gradient and therefore increases the potential for epithelial cell injuries in applications to lung airway mucus plugs. The topology of the yield surfaces as well as the flow pattern in the bubble frame of reference varies significantly by Ca and Bn.

Soil water retention and maximum capillary drive from saturation to oven dryness

USGS Publications Warehouse

Morel-Seytoux, Hubert J.; Nimmo, John R.

1999-01-01

This paper provides an alternative method to describe the water retention curve over a range of water contents from saturation to oven dryness. It makes two modifications to the standard Brooks and Corey [1964] (B-C) description, one at each end of the suction range. One expression proposed by Rossi and Nimmo [1994] is used in the high-suction range to a zero residual water content. (This Rossi-Nimmo modification to the Brooks-Corey model provides a more realistic description of the retention curve at low water contents.) Near zero suction the second modification eliminates the region where there is a change in suction with no change in water content. Tests on seven soil data sets, using three distinct analytical expressions for the high-, medium-, and low-suction ranges, show that the experimental water retention curves are well fitted by this composite procedure. The high-suction range of saturation contributes little to the maximum capillary drive, defined with a good approximation for a soil water and air system as HcM = ∫0∞ Krwdhc , where krw is relative permeability (or conductivity) to water and hc is capillary suction, a positive quantity in unsaturated soils. As a result, the modification suggested to describe the high-suction range does not significantly affect the equivalence between Brooks-Corey (B-C) and van Genuchten [1980] parameters presented earlier. However, the shape of the retention curve near “natural saturation” has a significant impact on the value of the capillary drive. The estimate using the Brooks-Corey power law, extended to zero suction, will exceed that obtained with the new procedure by 25 to 30%. It is not possible to tell which procedure is appropriate. Tests on another data set, for which relative conductivity data are available, support the view of the authors that measurements of a retention curve coupled with a speculative curve of relative permeability as from a capillary model are not sufficient to accurately determine the (maximum) capillary drive. The capillary drive is a dynamic scalar, whereas the retention curve is of a static character. Only measurements of infiltration rates with time can determine the capillary drive with precision for a given soil.

A compartment model of alveolar-capillary oxygen diffusion with ventilation-perfusion gradient and dynamics of air transport through the respiratory tract.

PubMed

Jaworski, Jacek; Redlarski, Grzegorz

2014-08-01

This paper presents a model of alveolar-capillary oxygen diffusion with dynamics of air transport through the respiratory tract. For this purpose electrical model representing the respiratory tract mechanics and differential equations representing oxygen membrane diffusion are combined. Relevant thermodynamic relations describing the mass of oxygen transported into the human body are proposed as the connection between these models, as well as the influence of ventilation-perfusion mismatch on the oxygen diffusion. The model is verified based on simulation results of varying exercise intensities and statistical calculations of the results obtained during various clinical trials. The benefit of the approach proposed is its application in simulation-based research aimed to generate quantitative data of normal and pathological conditions. Based on the model presented, taking into account many essential physiological processes and air transport dynamics, comprehensive and combined studies of the respiratory efficiency can be performed. The impact of physical exercise, precise changes in respiratory tract mechanics and alterations in breathing pattern can be analyzed together with the impact of various changes in alveolar-capillary oxygen diffusion. This may be useful in simulation of effects of many severe medical conditions and increased activity level. Copyright © 2014 Elsevier Ltd. All rights reserved.

Generalized network modeling of capillary-dominated two-phase flow

NASA Astrophysics Data System (ADS)

Raeini, Ali Q.; Bijeljic, Branko; Blunt, Martin J.

2018-02-01

We present a generalized network model for simulating capillary-dominated two-phase flow through porous media at the pore scale. Three-dimensional images of the pore space are discretized using a generalized network—described in a companion paper [A. Q. Raeini, B. Bijeljic, and M. J. Blunt, Phys. Rev. E 96, 013312 (2017), 10.1103/PhysRevE.96.013312]—which comprises pores that are divided into smaller elements called half-throats and subsequently into corners. Half-throats define the connectivity of the network at the coarsest level, connecting each pore to half-throats of its neighboring pores from their narrower ends, while corners define the connectivity of pore crevices. The corners are discretized at different levels for accurate calculation of entry pressures, fluid volumes, and flow conductivities that are obtained using direct simulation of flow on the underlying image. This paper discusses the two-phase flow model that is used to compute the averaged flow properties of the generalized network, including relative permeability and capillary pressure. We validate the model using direct finite-volume two-phase flow simulations on synthetic geometries, and then present a comparison of the model predictions with a conventional pore-network model and experimental measurements of relative permeability in the literature.

Comparison of CO2 trapping in highly heterogeneous reservoirs with Brooks-Corey and van Genuchten capillary pressure curves

NASA Astrophysics Data System (ADS)

Gershenzon, Naum; Soltanian, Mohamadreza; Ritzi, Robert, Jr.; Dominic, David

2015-04-01

Geological heterogeneities essentially affect the dynamics of a CO2 plume in subsurface environments. Recent studies have led to new conceptual and quantitative models for sedimentary architecture in fluvial deposits over a range of scales that are relevant to the performance of some deep saline reservoirs [1, 2]. Previously we showed how the dynamics of a CO2 plume, during and after injection, is influenced by the hierarchical and multi-scale stratal architecture in such reservoirs [3]. The results strongly suggest that representing these small scales (few cm in vertical direction and few meters in horizontal direction) features and representing how they are organized within a hierarchy of larger-scale features, is critical to understanding capillary trapping processes. The results also demonstrated the importance of using separate capillary pressure and relative permeability relationships for different textural facies types. Here we present the result of simulation of CO2 trapping in deep saline aquifers using two different conventional approaches, i.e. Brooks-Corey and van Genuchten, to capillary pressure. We showed that capillary trapping as well as dissolution rates are very different for the Brooks-Corey and van Genuchten approaches if reservoir consists from various species with different capillary pressure and relative permeability curves. We also found a dramatic difference in simulation time; using the van Genuchten approach improves convergence and thus reduces calculation time by one-two orders of magnitude. [1] Bridge, J.S. (2006), Fluvial facies models: Recent developments, in Facies Models Revisited, SEPM Spec. Publ., 84, edited by H. W. Posamentier and R. G. Walker, pp. 85-170, Soc. for Sediment. Geol. (SEPM), Tulsa, Okla [2] Ramanathan, R., A. Guin, R.W. Ritzi, D.F. Dominic, V.L. Freedman, T.D. Scheibe, and I.A. Lunt (2010), Simulating the heterogeneity in channel belt deposits: Part 1. A geometric-based methodology and code, Water Resources Research, v. 46, W04515. [3] Gershenzon N.I., M. Soltanian, R.W. Ritzi Jr., and D.F. Dominic (2014) Influence of small scale heterogeneity on CO2 trapping processes in deep saline aquifers, Energy Procedia, 59, 166 - 173.

Motion of liquid plugs between vapor bubbles in capillary tubes: a comparison between fluids

NASA Astrophysics Data System (ADS)

Bertossi, Rémi; Ayel, Vincent; Mehta, Balkrishna; Romestant, Cyril; Bertin, Yves; Khandekar, Sameer

2017-11-01

Pulsating heat pipes (PHP) are now well-known devices in which liquid/vapor slug flow oscillates in a capillary tube wound between hot and cold sources. In this context, this paper focuses on the motion of the liquid plug, trapped between vapor bubbles, moving in capillary tubes, to try to better understand the thermo-physical phenomena involved in such devices. This study is divided into three parts. In the first part, an experimental study presents the evolution of the vapor pressure during the evaporation process of a liquid thin film deposited from a liquid plug flowing in a heated capillary tube: it is found that the behavior of the generated and removed vapor can be very different, according to the thermophysical properties of the fluids. In the second part, a transient model allows to compare, in terms of pressure and duration, the motion of a constant-length liquid plug trapped between two bubbles subjected to a constant difference of vapor pressure: the results highlight that the performances of the four fluids are also very different. Finally, a third model that can be considered as an improvement of the second one, is also presented: here, the liquid slug is surrounded by two vapor bubbles, one subjected to evaporation, the pressure in both bubbles is now a result of the calculation. This model still allows comparing the behaviors of the fluid. Even if our models are quite far from a complete model of a real PHP, results do indicate towards the applicability of different fluids as suitable working fluids for PHPs, particularly in terms of the flow instabilities which they generate.

Modeling diffusion and reaction in soils: 9. The Buckingham-Burdine-Campbell equation for gas diffusivity in undisturbed soil

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moldrup, P.; Olesen, T.; Yamaguchi, T.

1999-08-01

Accurate description of gas diffusivity (ratio of gas diffusion coefficients in soil and free air, D{sub s}/D{sub 0}) in undisturbed soils is a prerequisite for predicting in situ transport and fate of volatile organic chemicals and greenhouse gases. Reference point gas diffusivities (R{sub p}) in completely dry soil were estimated for 20 undisturbed soils by assuming a power function relation between gas diffusivity and air-filled porosity ({epsilon}). Among the classical gas diffusivity models, the Buckingham (1904) expression, equal to the soil total porosity squared, best described R{sub p}. Inasmuch, as their previous works implied a soil-type dependency of D{sub s}/D{submore » 0}({epsilon}) in undisturbed soils, the Buckingham R{sub p} expression was inserted in two soil-type-dependent D{sub s}/D{sub 0}({epsilon}) models. One D{sub s}/D{sub 0}({epsilon}) model is a function of pore-size distribution (the Campbell water retention parameter used in a modified Burdine capillary tube model), and the other is a calibrated, empirical function of soil texture (silt + sand fraction). Both the Buckingham-Burdine-Campbell (BBC) and the Buckingham/soil texture-based D{sub s}/D{sub 0}({epsilon}) models described well the observed soil type effects on gas diffusivity and gave improved predictions compared with soil type independent models when tested against an independent data set for six undisturbed surface soils. This study emphasizes that simple but soil-type-dependent power function D{sub s}/D{sub 0}({epsilon}) models can adequately describe and predict gas diffusivity in undisturbed soil. The authors recommend the new BBC model as basis for modeling gas transport and reactions in undisturbed soil systems.« less

Vadose zone microbiology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kieft, Thomas L.; Brockman, Fred J.

2001-01-17

The vadose zone is defined as the portion of the terrestrial subsurface that extends from the land surface downward to the water table. As such, it comprises the surface soil (the rooting zone), the underlying subsoil, and the capillary fringe that directly overlies the water table. The unsaturated zone between the rooting zone and the capillary fringe is termed the "intermediate zone" (Chapelle, 1993). The vadose zone has also been defined as the unsaturated zone, since the sediment pores and/or rock fractures are generally not completely water filled, but instead contain both water and air. The latter characteristic results inmore » the term "zone of aeration" to describe the vadose zone. The terms "vadose zone," "unsaturated zone", and "zone of aeration" are nearly synonymous, except that the vadose zone may contain regions of perched water that are actually saturated. The term "subsoil" has also been used for studies of shallow areas of the subsurface immediately below the rooting zone. This review focuses almost exclusively on the unsaturated region beneath the soil layer since there is already an extensive body of literature on surface soil microbial communities and process, e.g., Paul and Clark (1989), Metting (1993), Richter and Markowitz, (1995), and Sylvia et al. (1998); whereas the deeper strata of the unsaturated zone have only recently come under scrutiny for their microbiological properties.« less

Fast-neutron and gamma-ray imaging with a capillary liquid xenon converter coupled to a gaseous photomultiplier

NASA Astrophysics Data System (ADS)

Israelashvili, I.; Coimbra, A. E. C.; Vartsky, D.; Arazi, L.; Shchemelinin, S.; Caspi, E. N.; Breskin, A.

2017-09-01

Gamma-ray and fast-neutron imaging was performed with a novel liquid xenon (LXe) scintillation detector read out by a Gaseous Photomultiplier (GPM). The 100 mm diameter detector prototype comprised a capillary-filled LXe converter/scintillator, coupled to a triple-THGEM imaging-GPM, with its first electrode coated by a CsI UV-photocathode, operated in Ne/5%CH4 at cryogenic temperatures. Radiation localization in 2D was derived from scintillation-induced photoelectron avalanches, measured on the GPM's segmented anode. The localization properties of 60Co gamma-rays and a mixed fast-neutron/gamma-ray field from an AmBe neutron source were derived from irradiation of a Pb edge absorber. Spatial resolutions of 12± 2 mm and 10± 2 mm (FWHM) were reached with 60Co and AmBe sources, respectively. The experimental results are in good agreement with GEANT4 simulations. The calculated ultimate expected resolutions for our application-relevant 4.4 and 15.1 MeV gamma-rays and 1-15 MeV neutrons are 2-4 mm and ~ 2 mm (FWHM), respectively. These results indicate the potential applicability of the new detector concept to Fast-Neutron Resonance Radiography (FNRR) and Dual-Discrete-Energy Gamma Radiography (DDEGR) of large objects.

Steady boiling of vapor bubbles in rectangular channels

NASA Astrophysics Data System (ADS)

Ajaev, Vladimir S.; Homsy, George M.

2000-11-01

We consider vapor bubbles in microchannels in which the vapor is produced by a heater element and condenses in cooler parts of the interface. The free boundary problem is formulated for a long steady-state bubble in a rectangular channel with a heated bottom. The shape of the liquid-vapor interface is described using lubrication-type equations in the regime in which the vapor phase fills most of the cross-section. Contact lines may be present, marking the transitions between molecularly thin films and macroscopic ones. The main parameters are the differences between heater, saturation, and top wall temperatures. The equations are solved numerically over a range of parameter values with an integral condition requiring the evaporation near the heater to balance condensation in colder areas of the interface. Depending on the temperature, the side walls can be either dry or covered with a liquid film; we identify criteria for these two different regimes. The asymptotic method breaks down in the limit when capillary condensation becomes important near the bubble top and a different approach is used to determine the shape of the bubble in this limit. Solutions here involve localized regions of large mass fluxes, which are asymptotically matched to capillary-statics regions where the heat transfer is negligible.

Ionization of Xenon to the Nickel-Like Stage and Beyond in Micro-Capillary Plasma Columns Heated by Ultrafast Current Pulses

NASA Astrophysics Data System (ADS)

Avaria, G.; Grisham, M.; Li, J.; Tomasel, F. G.; Shlyapstsev, V. N.; Busquet, M.; Woolston, M.; Rocca, J. J.

Homogeneous plasma columns with ionization levels typical of MA discharges were created by rapidly heating gas-filled 520 µm diameter channels with ns rise-time current pulses of unusually low amplitude, 40 kA. These conditions allow the generation of high aspect ratio (eg. > 300:1) plasma columns with very high degrees of ionization (e.g. Ni-like Xenon) of interest for soft x-ray lasers below λ = 10 nm. Spectra and simulations of plasmas generated in 520 µm diameter alumina capillaries driven by 35-40 kA current pulses with 4 ns rise time were obtained for discharges in Xenon and Neon discharges. The first shows the presence of lines corresponding to ionization stages up to Fe-like Xe. The latter show that Al impurities from the walls are ionized to the H-like and He-like stages. He-like Al spectra containing the resonance line significantly broaden by opacity, the intercombination line, and Li-like satellites are analyzed. For Xenon discharges, the spectral lines from the Ni-like transitions the 3d94d (3/2, 3/2)J = 0 to the 3d94p(5/2, 3/2)J = 1 and to 3d94p(3/2, 1/2)J = 1 are observed.

Capillary pressure – saturation relationships for gas shales measured using a water activity meter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Donnelly, B.; Perfect, E.; McKay, L. D.

Hydraulic fracturing of gas shale formations involves pumping a large volume of fracking fluid into a hydrocarbon reservoir to fracture the rock and thus increase its permeability. The majority of the fracking fluid introduced is never recovered and the fate of this lost fluid, often called “leak off,” has become the source of much debate. Information on the capillary pressure – saturation relationship for each wetting phase is needed to simulate leak off using numerical reservoir models. The petroleum industry commonly employs air – water capillary pressure – saturation curves to predict these relationships for mixed wet reservoirs. Traditional methodsmore » of measuring this curve are unsuitable for gas shales due to high capillary pressures associated with the small pores present. Still, a possible alternative method is the water activity meter which is used widely in the soil sciences for such measurements. However, its application to lithified material has been limited. Here, this study utilized a water activity meter to measure air – water capillary pressures (ranging from 1.3 to 219.6 MPa) at several water saturation levels in both the wetting and drying directions. Water contents were measured gravimetrically. Seven types of gas producing shale with different porosities (2.5–13.6%) and total organic carbon contents (0.4–13.5%) were investigated. Nonlinear regression was used to fit the resulting capillary pressure – water saturation data pairs for each shale type to the Brooks and Corey equation. Data for six of the seven shale types investigated were successfully fitted (median R 2 = 0.93), indicating this may be a viable method for parameterizing capillary pressure – saturation relationships for inclusion in numerical reservoir models. As expected, the different shale types had statistically different Brooks and Corey parameters. However, there were no significant differences between the Brooks and Corey parameters for the wetting and drying measurements, suggesting that hysteresis may not need to be taken into account in leak off simulations.« less

Capillary pressure – saturation relationships for gas shales measured using a water activity meter

DOE PAGES

Donnelly, B.; Perfect, E.; McKay, L. D.; ...

2016-05-10

Hydraulic fracturing of gas shale formations involves pumping a large volume of fracking fluid into a hydrocarbon reservoir to fracture the rock and thus increase its permeability. The majority of the fracking fluid introduced is never recovered and the fate of this lost fluid, often called “leak off,” has become the source of much debate. Information on the capillary pressure – saturation relationship for each wetting phase is needed to simulate leak off using numerical reservoir models. The petroleum industry commonly employs air – water capillary pressure – saturation curves to predict these relationships for mixed wet reservoirs. Traditional methodsmore » of measuring this curve are unsuitable for gas shales due to high capillary pressures associated with the small pores present. Still, a possible alternative method is the water activity meter which is used widely in the soil sciences for such measurements. However, its application to lithified material has been limited. Here, this study utilized a water activity meter to measure air – water capillary pressures (ranging from 1.3 to 219.6 MPa) at several water saturation levels in both the wetting and drying directions. Water contents were measured gravimetrically. Seven types of gas producing shale with different porosities (2.5–13.6%) and total organic carbon contents (0.4–13.5%) were investigated. Nonlinear regression was used to fit the resulting capillary pressure – water saturation data pairs for each shale type to the Brooks and Corey equation. Data for six of the seven shale types investigated were successfully fitted (median R 2 = 0.93), indicating this may be a viable method for parameterizing capillary pressure – saturation relationships for inclusion in numerical reservoir models. As expected, the different shale types had statistically different Brooks and Corey parameters. However, there were no significant differences between the Brooks and Corey parameters for the wetting and drying measurements, suggesting that hysteresis may not need to be taken into account in leak off simulations.« less

Rough surface adhesion in the presence of capillary condensation

DOE PAGES

DelRio, Frank W.; Dunn, Martin L.; Phinney, Leslie M.; ...

2007-04-17

Capillary condensation of water can have a significant effect on rough surface adhesion. Here, to explore this phenomenon between micromachined surfaces, the authors perform microcantilever experiments as a function of surface roughness and relative humidity (RH). Below a threshold RH, the adhesion is mainly due to van der Waals forces across extensive noncontacting areas. Above the threshold RH, the adhesion jumps due to capillary condensation and increases towards the upper limit of Γ=144mJ/m 2. Lastly, a detailed model based on the measured surface topography qualitatively agrees with the experimental data only when the topographic correlations between the upper and lowermore » surfaces are considered.« less

Lysosomal Trapping Is Present in Retinal Capillary Endothelial Cells: Insight into Its Influence on Cationic Drug Transport at the Inner Blood-Retinal Barrier.

PubMed

Kubo, Yoshiyuki; Seko, Narumi; Usui, Takuya; Akanuma, Shin-Ichi; Hosoya, Ken-Ichi

2016-01-01

Lysosomal trapping was investigated in the retinal capillary endothelial cells that are responsible for the inner blood-retinal barrier (BRB) using LysoTracker(®) Red (LTR). Using confocal microscopy on TR-iBRB2 cells, an in vitro model of the inner BRB, the presence of lysosomal trapping in retinal capillary endothelial cells was suggested since TR-iBRB2 cells exhibited punctuate intracellular localization of LTR that was attenuated by NH4Cl treatment. The study confirmed that LTR uptake by retinal capillary endothelial cells took place in a time- and temperature-dependent manner, and exhibited the 1.58-fold greater uptake at pH 8.4 than that at pH 7.4 while there was no change in uptake between pH 6.4 and pH 7.4, suggesting that passive diffusion is not enough to explain LTR uptake. The inhibition study showed the possible influence of lysosomal trapping on cationic drug transport by retinal capillary endothelial cells since LTR uptake was significantly inhibited by cationic amphiphilic drugs. Inhibition profiling and the estimation of IC50 suggested the influence of lysosomal trapping on propranolol and low-affinity pyrilamine transport while lysosomal trapping had only a partial effect on verapamil, clonidine, nicotine and high-affinity pyrilamine transport in retinal capillary endothelial cells.

Capillary spreading of contact line over a sinking sphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Seong Jin; Fezzaa, Kamel; An, Jim

The contact line dynamics over a sinking solid sphere are investigated in comparison with classical spreading theories. Experimentally, high-speed imaging systems with optical light or x-ray illumination are employed to accurately measure the spreading motion and dynamic contact angle of the contact line. Millimetric spheres are controlled to descend with a constant speed ranging from 7.3 × 10-5 to 0.79 m/s. We observed three different spreading stages over a sinking sphere, which depends on the contact line velocity and contact angle. These stages consistently showed the characteristics of capillarity-driven spreading as the contact line spreads faster with a higher contactmore » angle. The contact line velocity is observed to follow a classical capillary-viscous model at a high Ohnesorge number (> 0.02). For the cases with a relatively low Ohnesorge number (< 0.02), the contact line velocity is significantly lower than the speed predicted by the capillary-viscous balance. This indicates the existence of an additional opposing force (inertia) for a decreasing Ohnesorge number. The capillary-inertial balance is only observed at the very beginning of the capillary rise, in which the maximum velocity is independent of the sphere’s sinking speed. Additionally, we observed the linear relation between the contact line velocity and the sphere sinking speed during the second stage, which represents capillary adjustment by dynamic contact angle.« less

Surface Properties of PEMFC Gas Diffusion Layers

DOE Office of Scientific and Technical Information (OSTI.GOV)

WoodIII, David L; Rulison, Christopher; Borup, Rodney

2010-01-01

The wetting properties of PEMFC Gas Diffusion Layers (GDLs) were quantified by surface characterization measurements and modeling of material properties. Single-fiber contact-angle and surface energy (both Zisman and Owens-Wendt) data of a wide spectrum of GDL types is presented to delineate the effects of hydrophobic post-processing treatments. Modeling of the basic sessile-drop contact angle demonstrates that this value only gives a fraction of the total picture of interfacial wetting physics. Polar forces are shown to contribute 10-20 less than dispersive forces to the composite wetting of GDLs. Internal water contact angles obtained from Owens-Wendt analysis were measured at 13-19 highermore » than their single-fiber counterparts. An inverse relationship was found between internal contact angle and both Owens-Wendt surface energy and % polarity of the GDL. The most sophisticated PEMFC mathematical models use either experimentally measured capillary pressures or the standard Young-Laplace capillary-pressure equation. Based on the results of the Owens-Wendt analysis, an advancement to the Young-Laplace equation is proposed for use in these mathematical models, which utilizes only solid surface energies and fractional surface coverage of fluoropolymer. Capillary constants for the spectrum of analyzed GDLs are presented for the same purpose.« less

Directional, passive liquid transport: the Texas horned lizard as a model for a biomimetic 'liquid diode'.

PubMed

Comanns, Philipp; Buchberger, Gerda; Buchsbaum, Andreas; Baumgartner, Richard; Kogler, Alexander; Bauer, Siegfried; Baumgartner, Werner

2015-08-06

Moisture-harvesting lizards such as the Texas horned lizard (Iguanidae: Phrynosoma cornutum) live in arid regions. Special skin adaptations enable them to access water sources such as moist sand and dew: their skin is capable of collecting and transporting water directionally by means of a capillary system between the scales. This fluid transport is passive, i.e. requires no external energy, and directs water preferentially towards the lizard's snout. We show that this phenomenon is based on geometric principles, namely on a periodic pattern of interconnected half-open capillary channels that narrow and widen. Following a biomimetic approach, we used these principles to develop a technical prototype design. Building upon the Young-Laplace equation, we derived a theoretical model for the local behaviour of the liquid in such capillaries. We present a global model for the penetration velocity validated by experimental data. Artificial surfaces designed in accordance with this model prevent liquid flow in one direction while sustaining it in the other. Such passive directional liquid transport could lead to process improvements and reduction of resources in many technical applications. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

Velocity dependence of biphasic flow structuration: steady-state and oscillating flow effects

NASA Astrophysics Data System (ADS)

Tore Tallakstad, Ken; Jankov, Mihailo; Løvoll, Grunde; Toussaint, Renaud; Jørgen Mâløy, Knut; Grude Flekkøy, Eirik; Schmittbuhl, Jean; Schäfer, Gerhard; Méheust, Yves; Arendt Knudsen, Henning

2010-05-01

We study various types of biphasic flows in quasi-two-dimensional transparent porous models. These flows imply a viscous wetting fluid, and a lowly viscous one. The models are transparent, allowing the displacement process and structure to be monitored in space and time. Three different aspects will be presented: 1. In stationary biphasic flows, we study the relationship between the macroscopic pressure drop (related to relative permeability) and the average flow rate, and how this arises from the cluster size distribution of the lowly viscous fluid [1]. 2. In drainage situations, we study how the geometry of the invader can be explained, and how it gives rise to apparent dynamic capillary effects. We show how these can be explained by viscous effects on evolving geometries of invading fluid [2]. 3. We study the impact of oscillating pressure fields superimposed to a background flow over the flow regimes patterns [3]. Steady-State Two-Phase Flow in Porous Media: Statistics and Transport Properties. First, in stationary flow with a control of the flux of both fluids, we show how the pressure drop depends on the flow rate. We will show that the dynamics is dominated by the interplay between a viscous pressure field from the wetting fluid and bubble transport of a less viscous, nonwetting phase. In contrast with more studied displacement front systems, steady-state flow is in equilibrium, statistically speaking. The corresponding theoretical simplicity allows us to explain a data collapse in the cluster size distribution of lowly viscous fluid in the system, as well as the relation |?P|∞√Ca--. This allows to explain so called relative permeability effects by the morphological changes of the cluster size distribution. Influence of viscous fingering on dynamic saturation-pressure curves in porous media. Next, we study drainage in such models, and investigate the relationship between the pressure field and the morphology of the invading fluid. This allows to model the impact of the saturation changes in the system over the pressure difference between the wetting and non wetting phase. We show that the so-called dynamic effects referred in the hydrology literature of experimentally measured capillary pressure curves might be explained by the combined effect of capillary pressure along the invasion front of the gaseous phase and pressure changes caused by viscous effects. A detailed study of the structure optically followed shows that the geometry of the invader is self-similar with two different behaviors at small and large scales: the structure corresponds to the ones of invasion percolation models at small scales (capillary fingering structures with fractal dimension D=1.83), whereas at large scales, viscous pressure drops dominate over the capillary threshold variations, and the structures are self-similar fingering structures with a fractal dimension corresponding to Dielectric Breakdown Models (variants of the DLA model), with D ≠ƒ 1.5. The cross-over scale is set by the scale at which capillary fluctuations are of the order of the viscous pressure drops. This leads physically to the fact that cross-over scale between the two fingering dimensions, goes like the inverse of the capillary number. This study utilizes these geometrical characteristics of the viscous fingers forming in dynamic drainage, to obtain a meaningfull scaling law for the saturation-pressure curve at finite speed, i.e. the so-called dynamic capillary pressure relations. We thus show how the micromechanical interplay between viscous and capillary forces leads to some pattern formation, which results in a general form of dynamic capillary pressure relations. By combining these detailed informations on the displacement structure with global measures of pressure, saturation and controlling the capillary number Ca, a scaling relation relating pressure, saturation, system size and capillary number is developed. By applying this scaling relation, pressure-saturation curves for a wide range of capillary numbers can be collapsed. Effects of pressure oscillations on drainage in an elastic porous medium: The effects of seismic stimulation on the flow of two immiscible fluids in an elastic synthetic porous medium is experimentally investigated. A wetting fluid is slowly evacuated from the medium, while a pressure oscillation is applied on the injected non-wetting fluid. The amplitude and frequency of the pressure oscillations as well as the evacuation speed are kept constant throughout an experiment. The resulting morphology of the invading structure is found to be strongly dependent on the interplay between the amplitude and the frequency of the applied pressure oscillations and the elasticity of the porous medium. Different combinations of these properties yield morphologically similar structures, allowing a classification of structures that is found to depend on a proposed dimensionless number. [1] Tallakstad, K.T., H.A. Knudsen, T. Ramstad, G. Løvoll, K.J. Maløy, R. Toussaint and E.G. Flekkøy , Steady-state two-phase flow in porous media: statistics and transport properties, Phys. Rev. Lett. 102, 074502 (2009). doi:10.1103/PhysRevLett.102.074502 [2] Løvoll, G., M. Jankov, K.J. Maløy, R. Toussaint, J. Schmittbuhl, G. Schaefer and Y. Ḿ eheust, Influence of viscous fingering on dynamic saturation-pressure curves in porous media, submitted to Transport In Porous Media, (2010) [3] Jankov, M., G. Løvoll, H.A. Knudsen, K.J. Maløy, R. Planet, R. Toussaint and E.G. Flekkøy; Effects of pressure oscillations on drainage in an elastic porous medium, Transport In Porous Media, in press (2010).

Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study.

PubMed

Cheng, Mengxia; Chen, Zilin

2017-08-01

A trypsin immobilized enzyme microreactor was successfully prepared in capillary for studying enzyme kinetics of trypsin and online screening of trypsin inhibitors from traditional Chinese medicine through capillary electrophoresis. Trypsin was immobilized on the inner wall at the inlet of the capillary treated with polydopamine. The rest of the capillary was used as a separation channel. The parameters including the separation efficiency and the activity of immobilized trypsin were comprehensively evaluated. Under the optimal conditions, online screening of trypsin inhibitors each time can be carried out within 6 min. The Michaelis-Menten constant of immobilized trypsin was calculated to be 0.50 mM, which indicated high affinity of the immobilized trypsin for the substrate. The half-maximal inhibitory concentration of known inhibitor of benzamidine hydrochloride hydrate as a model inhibitor was 13.32 mM. The proposed method was successfully applied to screen trypsin inhibitors from 15 compounds of traditional Chinese medicine. It has been found that baicalin showed inhibitory potency. Molecular docking study well supported the experimental result by exhibiting molecular interaction between enzyme and inhibitors. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Control of evaporation by geometry in capillary structures. From confined pillar arrays in a gap radial gradient to phyllotaxy-inspired geometry.

PubMed

Chen, Chen; Duru, Paul; Joseph, Pierre; Geoffroy, Sandrine; Prat, Marc

2017-11-08

Evaporation is a key phenomenon in the natural environment and in many technological systems involving capillary structures. Understanding the evaporation front dynamics enables the evaporation rate from microfluidic devices and porous media to be finely controlled. Of particular interest is the ability to control the position of the front through suitable design of the capillary structure. Here, we show how to design model capillary structures in microfluidic devices so as to control the drying kinetics. This is achieved by acting on the spatial organization of the constrictions that influence the invasion of the structure by the gas phase. Two types of control are demonstrated. The first is intended to control the sequence of primary invasions through the pore space, while the second aims to control the secondary liquid structures: films, bridges, etc., that can form in the region of pore space invaded by the gas phase. It is shown how the latter can be obtained from phyllotaxy-inspired geometry. Our study thus opens up a route toward the control of the evaporation kinetics by means of tailored capillary structures.

Fractal Analysis of Permeability of Unsaturated Fractured Rocks

PubMed Central

Jiang, Guoping; Shi, Wei; Huang, Lili

2013-01-01

A physical conceptual model for water retention in fractured rocks is derived while taking into account the effect of pore size distribution and tortuosity of capillaries. The formula of calculating relative hydraulic conductivity of fractured rock is given based on fractal theory. It is an issue to choose an appropriate capillary pressure-saturation curve in the research of unsaturated fractured mass. The geometric pattern of the fracture bulk is described based on the fractal distribution of tortuosity. The resulting water content expression is then used to estimate the unsaturated hydraulic conductivity of the fractured medium based on the well-known model of Burdine. It is found that for large enough ranges of fracture apertures the new constitutive model converges to the empirical Brooks-Corey model. PMID:23690746

Fractal analysis of permeability of unsaturated fractured rocks.

PubMed

Jiang, Guoping; Shi, Wei; Huang, Lili

2013-01-01

A physical conceptual model for water retention in fractured rocks is derived while taking into account the effect of pore size distribution and tortuosity of capillaries. The formula of calculating relative hydraulic conductivity of fractured rock is given based on fractal theory. It is an issue to choose an appropriate capillary pressure-saturation curve in the research of unsaturated fractured mass. The geometric pattern of the fracture bulk is described based on the fractal distribution of tortuosity. The resulting water content expression is then used to estimate the unsaturated hydraulic conductivity of the fractured medium based on the well-known model of Burdine. It is found that for large enough ranges of fracture apertures the new constitutive model converges to the empirical Brooks-Corey model.

Wet powder seal for gas containment

DOEpatents

Stang, Louis G.

1982-01-01

A gas seal is formed by a compact layer of an insoluble powder and liquid filling the fine interstices of that layer. The smaller the particle size of the selected powder, such as sand or talc, the finer will be the interstices or capillary spaces in the layer and the greater will be the resulting sealing capacity, i.e., the gas pressure differential which the wet powder layer can withstand. Such wet powder seal is useful in constructing underground gas reservoirs or storage cavities for nuclear wastes as well as stopping leaks in gas mains buried under ground or situated under water. The sealing capacity of the wet powder seal can be augmented by the hydrostatic head of a liquid body established over the seal.

Wet powder seal for gas containment

DOEpatents

Stang, L.G.

1979-08-29

A gas seal is formed by a compact layer of an insoluble powder and liquid filling the fine interstices of that layer. The smaller the particle size of the selected powder, such as sand or talc, the finer will be the interstices or capillary spaces in the layer and the greater will be the resulting sealing capacity, i.e., the gas pressure differential which the wet powder layer can withstand. Such wet powder seal is useful in constructing underground gas reservoirs or storage cavities for nuclear wastes as well as stopping leaks in gas mains buried under ground or situated under water. The sealing capacity of the wet powder seal can be augmented by the hydrostatic head of a liquid body established over the seal.

Method of forming and assembly of metal and ceramic parts

DOEpatents

Ripley, Edward B

2014-04-22

A method of forming and assembling at least two parts together that may be metal, ceramic, or a combination of metal and ceramic parts. Such parts may have different CTE. Individual parts that are formed and sintered from particles leave a network of interconnecting porosity in each sintered part. The separate parts are assembled together and then a fill material is infiltrated into the assembled parts using a method such as capillary action, gravity, and/or pressure. The assembly is then cured to yield a bonded and fully or near-fully dense part that has the desired physical and mechanical properties for the part's intended purpose. Structural strength may be added to the parts by the inclusion of fibrous materials.

Method of forming and assembly of metal parts and ceramic parts

DOEpatents

Ripley, Edward B [Knoxville, TN

2011-11-22

A method of forming and assembling at least two parts together that may be metal, ceramic, or a combination of metal and ceramic parts. Such parts may have different CTE. Individual parts that are formed and sintered from particles leave a network of interconnecting porosity in each sintered part. The separate parts are assembled together and then a fill material is infiltrated into the assembled parts using a method such as capillary action, gravity, and/or pressure. The assembly is then cured to yield a bonded and fully or near-fully dense part that has the desired physical and mechanical properties for the part's intended purpose. Structural strength may be added to the parts by the inclusion of fibrous materials.

A three-compartment model of osmotic water exchange in the lung microvasculature.

PubMed

Seale, K T; Harris, T R

2000-08-01

A bolus injection of hypertonic NaCl into the pulmonary arterial circulation of an isolated perfused dog lung causes the osmotic movement of water first into, and then out of the capillary. The associated changes in blood constituent concentrations and density are referred to as the osmotic transient (OT). Measurement of the sound conduction velocity of effluent blood during an OT is a highly sensitive way to monitor water movement between the vascular and extravascular spaces. It was our objective to develop a mathematical model that adequately describes this transient change in the sound conduction velocity and evaluate its application under conditions of homogeneous and heterogeneous capillary flow distributions. The model accounts for osmotic water exchange between the capillary and two parallel extravascular compartments, and includes as parameters the osmotic conductances (sigmaK1 ,sigmaK2) of the two compartments. The osmotic conductance parameters incorporate the filtration coefficient for water and reflection coefficient for salt for the two pathways of water exchange. The partition of total extravascular lung water (EVLW) between the two extravascular compartments is a third parameter of the model. The homogeneous model parameter estimates (per gram wet lung weight +/-95% confidence limits) from the best-fit analysis of a typical curve were sigmaK1=2.15 +/-0.07, sigmaK2 = 0.03 + 0.00 [ml h(-1) (mosmol/liter)(-1) g(-1)] and V1 = 23.83+/-0.12 ml, with a coefficient of variation (CV) of 0.08. The heterogeneous parameter estimates for a capillary transit time distribution with mean transit time (MTTc) = 1.72 s, and relative dispersion (RDc) = 0.35 were KI = 2.38+/-0.05, or K2 = 0.03+/-0.00 [ml h(-1) (mosmol/liter)(-1) g(-1)], V1 = 23.91+/-0.08 ml, and CV=0.05. EVLW was 42.1 ml for both models. We conclude that the three-compartment mathematical model adequately describes a typical OT under both homogeneous and heterogeneous blood flow assumptions.

The roles of cerebral blood flow, capillary transit time heterogeneity, and oxygen tension in brain oxygenation and metabolism

PubMed Central

Jespersen, Sune N; Østergaard, Leif

2012-01-01

Normal brain function depends critically on moment-to-moment regulation of oxygen supply by the bloodstream to meet changing metabolic needs. Neurovascular coupling, a range of mechanisms that converge on arterioles to adjust local cerebral blood flow (CBF), represents our current framework for understanding this regulation. We modeled the combined effects of CBF and capillary transit time heterogeneity (CTTH) on the maximum oxygen extraction fraction (OEFmax) and metabolic rate of oxygen that can biophysically be supported, for a given tissue oxygen tension. Red blood cell velocity recordings in rat brain support close hemodynamic–metabolic coupling by means of CBF and CTTH across a range of physiological conditions. The CTTH reduction improves tissue oxygenation by counteracting inherent reductions in OEFmax as CBF increases, and seemingly secures sufficient oxygenation during episodes of hyperemia resulting from cortical activation or hypoxemia. In hypoperfusion and states of blocked CBF, both lower oxygen tension and CTTH may secure tissue oxygenation. Our model predicts that disturbed capillary flows may cause a condition of malignant CTTH, in which states of higher CBF display lower oxygen availability. We propose that conditions with altered capillary morphology, such as amyloid, diabetic or hypertensive microangiopathy, and ischemia–reperfusion, may disturb CTTH and thereby flow-metabolism coupling and cerebral oxygen metabolism. PMID:22044867

Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels.

PubMed

Wang, Xiaolin; Phan, Duc T T; Sobrino, Agua; George, Steven C; Hughes, Christopher C W; Lee, Abraham P

2016-01-21

This paper reports a method for generating an intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This platform incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, sprouting angiogenesis, and anastomosis in sequential order. After formation of a capillary network inside the tissue chamber via vasculogenesis, the adjacent microfluidic channels are lined with a monolayer of ECs, which then serve as the high-pressure input ("artery") and low pressure output ("vein") conduits. To promote a tight interconnection between the artery/vein and the capillary network, sprouting angiogenesis is induced, which promotes anastomosis of the vasculature inside the tissue chamber with the EC lining along the microfluidic channels. Flow of fluorescent microparticles confirms the perfusability of the lumenized microvascular network, and minimal leakage of 70 kDa FITC-dextran confirms physiologic tightness of the EC junctions and completeness of the interconnections between artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological transport model of interconnected perfused vessels from artery to vascularized tissue to vein. The system has utility in a wide range of organ-on-a-chip applications as it enables the physiological vascular interconnection of multiple on-chip tissue constructs that can serve as disease models for drug screening.

US-SOMO HPLC-SAXS module: dealing with capillary fouling and extraction of pure component patterns from poorly resolved SEC-SAXS data

PubMed Central

Brookes, Emre; Vachette, Patrice; Rocco, Mattia; Pérez, Javier

2016-01-01

Size-exclusion chromatography coupled with SAXS (small-angle X-ray scattering), often performed using a flow-through capillary, should allow direct collection of monodisperse sample data. However, capillary fouling issues and non-baseline-resolved peaks can hamper its efficacy. The UltraScan solution modeler (US-SOMO) HPLC-SAXS (high-performance liquid chromatography coupled with SAXS) module provides a comprehensive framework to analyze such data, starting with a simple linear baseline correction and symmetrical Gaussian decomposition tools [Brookes, Pérez, Cardinali, Profumo, Vachette & Rocco (2013 ▸). J. Appl. Cryst. 46, 1823–1833]. In addition to several new features, substantial improvements to both routines have now been implemented, comprising the evaluation of outcomes by advanced statistical tools. The novel integral baseline-correction procedure is based on the more sound assumption that the effect of capillary fouling on scattering increases monotonically with the intensity scattered by the material within the X-ray beam. Overlapping peaks, often skewed because of sample interaction with the column matrix, can now be accurately decomposed using non-symmetrical modified Gaussian functions. As an example, the case of a polydisperse solution of aldolase is analyzed: from heavily convoluted peaks, individual SAXS profiles of tetramers, octamers and dodecamers are extracted and reliably modeled. PMID:27738419

Transport of volatile organic compounds across the capillary fringe

USGS Publications Warehouse

McCarthy, Kathleen A.; Johnson, Richard L.

1993-01-01

Physical experiments were conducted to investigate the transport of a dissolved volatile organic compound (trichloroethylene, TCE) from shallow groundwater to the unsaturated zone under a variety of conditions including changes in the soil moisture profile and water table position. Experimental data indicated that at moderate groundwater velocities (0.1 m/d), vertical mechanical dispersion was negligible and molecular diffusion was the dominant vertical transport mechanism. Under these conditions, TCE concentrations decreased nearly 3 orders of magnitude across the capillary fringe and soil gas concentrations remained low relative to those of underlying groundwater. Data collected during a water table drop showed a short-term increase in concentrations throughout most of the unsaturated zone, but these concentrations quickly declined and approached initial values after the water table was returned to its original level. In the deep part of the unsaturated zone, the water table drop resulted in a long-term decrease in concentrations, illustrating the effects of hysteresis in the soil moisture profile. A two-dimensional random walk advection-diffusion model was developed to simulate the experimental conditions, and numerical simulations agreed well with experimental data. A simpler, one-dimensional finite-difference diffusion-dispersion model was also developed. One-dimensional simulations based on molecular diffusion also agreed well with experimental data. Simulations which incorporated mechanical dispersion tended to overestimate flux across the capillary fringe. Good agreement between the one- and two-dimensional models suggested that a simple, one-dimensional approximation of vertical transport across the capillary fringe can be useful when conditions are appropriate.

Thermal Integration of a Liquid Acquisition Device into a Cryogenic Feed System

NASA Technical Reports Server (NTRS)

Hastings, L. J.; Bolshinskiy, L. G.; Schunk, R. G.; Martin, A. K.; Eskridge, R. H.; Frenkel, A.; Grayson, G.; Pendleton, M. L.

2011-01-01

Primary objectives of this effort were to define the following: (1) Approaches for quantification of the accumulation of thermal energy within a capillary screen liquid acquisition device (LAD) for a lunar lander upper stage during periods of up to 210 days on the lunar surface, (2) techniques for mitigating heat entrapment, and (3) perform initial testing, data evaluation. The technical effort was divided into the following categories: (1) Detailed thermal modeling of the LAD/feed system interactions using both COMSOL computational fluid device and standard codes, (2) FLOW-3D modeling of bulk liquid to provide interfacing conditions for the LAD thermal modeling, (3) condensation conditioning of capillary screens to stabilize surface tension retention capability, and (4) subscale testing of an integrated LAD/feed system. Substantial progress was achieved in the following technical areas: (1) Thermal modeling and experimental approaches for evaluating integrated cryogen LAD/feed systems, at both the system and component levels, (2) reduced gravity pressure control analyses, (3) analytical modeling and testing for capillary screen conditioning using condensation and wicking, and (4) development of rapid turnaround testing techniques for evaluating LAD/feed system thermal and fluid integration. A comprehensive effort, participants included a diverse cross section of representatives from academia, contractors, and multiple Marshall Space Flight Center organizations.

Kinetics of gravity-driven slug flow in partially wettable capillaries of varying cross section

NASA Astrophysics Data System (ADS)

Nissan, Alon; Wang, Qiuling; Wallach, Rony

2016-11-01

A mathematical model for slug (finite liquid volume) motion in not-fully-wettable capillary tubes with sinusoidally varying cross-sectional areas was developed. The model, based on the Navier-Stokes equation, accounts for the full viscous terms due to nonuniform geometry, the inertial term, the slug's front and rear meniscus hysteresis effect, and dependence of contact angle on flow velocity (dynamic contact angle). The model includes a velocity-dependent film that is left behind the advancing slug, reducing its mass. The model was successfully verified experimentally by recording slug movement in uniform and sinusoidal capillary tubes with a gray-scale high-speed camera. Simulation showed that tube nonuniformity has a substantial effect on slug flow pattern: in a uniform tube it is monotonic and depends mainly on the slug's momentary mass/length; an undulating tube radius results in nonmonotonic flow characteristics. The static nonzero contact angle varies locally in nonuniform tubes owing to the additional effect of wall slope. Moreover, the nonuniform cross-sectional area induces slug acceleration, deceleration, blockage, and metastable-equilibrium locations. Increasing contact angle further amplifies the geometry effect on slug propagation. The developed model provides a modified means of emulating slug flow in differently wettable porous media for intermittent inlet water supply (e.g., raindrops on the soil surface).

Paramecium swimming in a capillary tube

NASA Astrophysics Data System (ADS)

Jana, Saikat; Jung, Sunghwan

2010-03-01

Micro-organisms exhibit different strategies for swimming in complex environments. Many micro-swimmers such as paramecium congregate and tend to live near wall. We investigate how paramecium moves in a confined space as compared to its motion in an unbounded fluid. A new theoretical model based on Taylor's sheet is developed, to study such boundary effects. In experiments, paramecia are put inside capillary tubes and their swimming behavior is observed. The data obtained from experiments is used to test the validity of our theoretical model and understand how the cilia influence the locomotion of paramecia in confined geometries.

A poroelastic medium saturated by a two-phase capillary fluid

NASA Astrophysics Data System (ADS)

Shelukhin, V. V.

2014-09-01

By Landau's approach developed for description of superfluidity of 2He, we derive a mathematical model for a poroelastic medium saturated with a two-phase capillary fluid. The model describes a three-velocity continuum with conservation laws which obey the basic principles of thermodynamics and which are consistent with the Galilean transformations. In contrast to Biot' linear theory, the equations derived allow for finite deformations. As the acoustic analysis reveals, there is one more longitudinal wave in comparison with the poroelastic medium saturated with a one-phase fluid. We prove that such a result is due to surface tension.

Modelling of hydrothermal instabilities in a capillary bridge

NASA Astrophysics Data System (ADS)

Pillai, Dipin; Wray, Alex; Narayanan, Ranga

2017-11-01

We examine the behaviour of a capillary bridge/boat suspended between two heated plates. Such systems are common in many physical situations such as crystal growth processes. However, as shown experimentally by Messmer et al., the system exhibits a complex array of behaviours driven by a Marangoni instability. While qualitative arguments have been advanced for these behaviours in the past, we develop a complete low-order model to elucidate the mechanisms at work. The model takes into account viscosity, surface tension, Marangoni stress and inertia as well as a full convection-diffusion equation for the thermal effects. Detailed comparisons of flow fields and thermal distributions are made with experiments. NASA NNX17AL27G and NSF 0968313.

Evaluation of the effects of insufficient blood volume samples on the performance of blood glucose self-test meters.

PubMed

Pfützner, Andreas; Schipper, Christina; Ramljak, Sanja; Flacke, Frank; Sieber, Jochen; Forst, Thomas; Musholt, Petra B

2013-11-01

Accuracy of blood glucose readings is (among other things) dependent on the test strip being completely filled with sufficient sample volume. The devices are supposed to display an error message in case of incomplete filling. This laboratory study was performed to test the performance of 31 commercially available devices in case of incomplete strip filling. Samples with two different glucose levels (60-90 and 300-350 mg/dl) were used to generate three different sample volumes: 0.20 µl (too low volume for any device), 0.32 µl (borderline volume), and 1.20 µl (low but supposedly sufficient volume for all devices). After a point-of-care capillary reference measurement (StatStrip, NovaBiomedical), the meter strip was filled (6x) with the respective volume, and the response of the meters (two devices) was documented (72 determinations/meter type). Correct response was defined as either an error message indicating incomplete filling or a correct reading (±20% compared with reference reading). Only five meters showed 100% correct responses [BGStar and iBGStar (both Sanofi), ACCU-CHEK Compact+ and ACCU-CHEK Mobile (both Roche Diagnostics), OneTouch Verio (LifeScan)]. The majority of the meters (17) had up to 10% incorrect reactions [predominantly incorrect readings with sufficient volume; Precision Xceed and Xtra, FreeStyle Lite, and Freedom Lite (all Abbott); GlucoCard+ and GlucoMen GM (both Menarini); Contour, Contour USB, and Breeze2 (all Bayer); OneTouch Ultra Easy, Ultra 2, and Ultra Smart (all LifeScan); Wellion Dialog and Premium (both MedTrust); FineTouch (Terumo); ACCU-CHEK Aviva (Roche); and GlucoTalk (Axis-Shield)]. Ten percent to 20% incorrect reactions were seen with OneTouch Vita (LifeScan), ACCU-CHEK Aviva Nano (Roche), OmniTest+ (BBraun), and AlphaChek+ (Berger Med). More than 20% incorrect reactions were obtained with Pura (Ypsomed), GlucoCard Meter and GlucoMen LX (both Menarini), Elite (Bayer), and MediTouch (Medisana). In summary, partial and incomplete blood filling of glucose meter strips is often associated with inaccurate reading. These findings underline the importance of appropriate patient education on this aspect of blood glucose self-monitoring. © 2013 Diabetes Technology Society.

Investigation of representing hysteresis in macroscopic models of two-phase flow in porous media using intermediate scale experimental data

NASA Astrophysics Data System (ADS)

Cihan, Abdullah; Birkholzer, Jens; Trevisan, Luca; Gonzalez-Nicolas, Ana; Illangasekare, Tissa

2017-01-01

Incorporating hysteresis into models is important to accurately capture the two phase flow behavior when porous media systems undergo cycles of drainage and imbibition such as in the cases of injection and post-injection redistribution of CO2 during geological CO2 storage (GCS). In the traditional model of two-phase flow, existing constitutive models that parameterize the hysteresis associated with these processes are generally based on the empirical relationships. This manuscript presents development and testing of mathematical hysteretic capillary pressure—saturation—relative permeability models with the objective of more accurately representing the redistribution of the fluids after injection. The constitutive models are developed by relating macroscopic variables to basic physics of two-phase capillary displacements at pore-scale and void space distribution properties. The modeling approach with the developed constitutive models with and without hysteresis as input is tested against some intermediate-scale flow cell experiments to test the ability of the models to represent movement and capillary trapping of immiscible fluids under macroscopically homogeneous and heterogeneous conditions. The hysteretic two-phase flow model predicted the overall plume migration and distribution during and post injection reasonably well and represented the postinjection behavior of the plume more accurately than the nonhysteretic models. Based on the results in this study, neglecting hysteresis in the constitutive models of the traditional two-phase flow theory can seriously overpredict or underpredict the injected fluid distribution during post-injection under both homogeneous and heterogeneous conditions, depending on the selected value of the residual saturation in the nonhysteretic models.

Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay.

PubMed

Yang, Zhanjun; Zong, Chen; Ju, Huangxian; Yan, Feng

2011-11-07

A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using α-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL(-1) and a low detection limit of 0.1 ng mL(-1). The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay. Copyright © 2011 Elsevier B.V. All rights reserved.

Extreme ultraviolet spectroscopy diagnostics of low-temperature plasmas based on a sliced multilayer grating and glass capillary optics.

PubMed

Kantsyrev, V L; Safronova, A S; Williamson, K M; Wilcox, P; Ouart, N D; Yilmaz, M F; Struve, K W; Voronov, D L; Feshchenko, R M; Artyukov, I A; Vinogradov, A V

2008-10-01

New extreme ultraviolet (EUV) spectroscopic diagnostics of relatively low-temperature plasmas based on the application of an EUV spectrometer and fast EUV diodes combined with glass capillary optics is described. An advanced high resolution dispersive element sliced multilayer grating was used in the compact EUV spectrometer. For monitoring of the time history of radiation, filtered fast EUV diodes were used in the same spectral region (>13 nm) as the EUV spectrometer. The radiation from the plasma was captured by using a single inexpensive glass capillary that was transported onto the spectrometer entrance slit and EUV diode. The use of glass capillary optics allowed placement of the spectrometer and diodes behind the thick radiation shield outside the direction of a possible hard x-ray radiation beam and debris from the plasma source. The results of the testing and application of this diagnostic for a compact laser plasma source are presented. Examples of modeling with parameters of plasmas are discussed.