In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis x C. dactylon) and their physiological responses to salt and drought stress.

PubMed

Lu, Shaoyun; Peng, Xinxiang; Guo, Zhenfei; Zhang, Gengyun; Wang, Zhongcheng; Wang, Congying; Pang, Chaoshu; Fan, Zhen; Wang, Jihua

2007-08-01

A protocol was established for in vitro selection of salinity tolerant somaclonal variations from suspension cultured calli of triploid bermudagrass cv. TifEagle. To induce somaclonal variations the calli were subcultured for 18 months and were then subject to three-round selections for salt-tolerant calli by placing on solid medium containing 0.3 M NaCl for 10 days followed by a recovery for 2 weeks. The surviving calli were regenerated on regeneration medium containing 0.1 M NaCl. Three somaclonal variant lines (2, 71, and 77) were obtained and analyzed. The selected somaclonal lines showed higher relative growth and less injury than TifEagle under salt stress, indicating that they increased salt tolerance. In addition, they had higher relative water content and lower electrolyte leakage than TifEagle after withholding irrigation, indicating that they also increased drought tolerance. The three somaclonal variant lines had higher proline content than TifEagle under normal growth condition. The line 71 had a higher K(+)/Na(+) ratio, whereas the lines 2 and 77 had higher CAT activity under control and salt stress conditions, indicating that different mechanisms for salt tolerance might exist in these three lines.

Monodispersed magnetite nanoparticles optimized for magnetic fluid hyperthermia: Implications in biological systems

NASA Astrophysics Data System (ADS)

Khandhar, Amit P.; Ferguson, R. Matthew; Krishnan, Kannan M.

2011-04-01

Magnetite (Fe3O4) nanoparticles (MNPs) are suitable materials for Magnetic Fluid Hyperthermia (MFH), provided their size is carefully tailored to the applied alternating magnetic field (AMF) frequency. Since aqueous synthesis routes produce polydisperse MNPs that are not tailored for any specific AMF frequency, we have developed a comprehensive protocol for synthesizing highly monodispersed MNPs in organic solvents, specifically tailored for our field conditions (f = 376 kHz, H0 = 13.4 kA/m) and subsequently transferred them to water using a biocompatible amphiphilic polymer. These MNPs (σavg. = 0.175) show truly size-dependent heating rates, indicated by a sharp peak in the specific loss power (SLP, W/g Fe3O4) for 16 nm (diameter) particles. For broader size distributions (σavg. = 0.266), we observe a 30% drop in overall SLP. Furthermore, heating measurements in biological medium [Dulbecco's modified Eagle medium (DMEM) + 10% fetal bovine serum] show a significant drop for SLP (˜30% reduction in 16 nm MNPs). Dynamic Light Scattering (DLS) measurements show particle hydrodynamic size increases over time once dispersed in DMEM, indicating particle agglomeration. Since the effective magnetic relaxation time of MNPs is determined by fractional contribution of the Neel (independent of hydrodynamic size) and Brownian (dependent on hydrodynamic size) components, we conclude that agglomeration in biological medium modifies the Brownian contribution and thus the net heating capacity of MNPs.

Spatial and temporal patterns in golden eagle diets in the western United States, with implications for conservation planning

USGS Publications Warehouse

Bedrosian, Geoffrey; Watson, James W.; Steenhof, Karen; Kochert, Michael N.; Preston, Charles R.; Woodbridge, Brian; Williams, Gary E.; Keller, Kent R.; Crandall, Ross H.

2017-01-01

Detailed information on diets and predatory ecology of Golden Eagles (Aquila chrysaetos) is essential to prioritize prey species management and to develop landscape-specific conservation strategies, including mitigation of the effects of energy development across the western United States. We compiled published and unpublished data on Golden Eagle diets to (1) summarize available information on Golden Eagle diets in the western U.S., (2) compare diets among biogeographic provinces, and (3) discuss implications for conservation planning and future research. We analyzed 35 studies conducted during the breeding season at 45 locations from 1940–2015. Golden Eagle diet differed among western ecosystems. Lower dietary breadth was associated with desert and shrub-steppe ecosystems and higher breadth with mountain ranges and the Columbia Plateau. Correlations suggest that percentage of leporids in the diet is the factor driving overall diversity of prey and percentage of other prey groups in the diet of Golden Eagles. Leporids were the primary prey of breeding Golden Eagles in 78% of study areas, with sciurids reported as primary prey in 18% of study areas. During the nonbreeding season, Golden Eagles were most frequently recorded feeding on leporids and carrion. Golden Eagles can be described as both generalist and opportunistic predators; they can feed on a wide range of prey species but most frequently feed on abundant medium-sized prey species in a given habitat. Spatial variations in Golden Eagle diet likely reflect regional differences in prey community, whereas temporal trends likely reflect responses to long-term change in prey populations. Evidence suggests dietary shifts from traditional (leporid) prey can have adverse effects on Golden Eagle reproductive rates. Land management practices that support or restore shrub-steppe ecosystem diversity should benefit Golden Eagles. More information is needed on nonbreeding-season diet to determine what food resources, such as carrion, are important for overwinter survival.

Upscaling pore pressure-dependent gas permeability in shales

NASA Astrophysics Data System (ADS)

Ghanbarian, Behzad; Javadpour, Farzam

2017-04-01

Upscaling pore pressure dependence of shale gas permeability is of great importance and interest in the investigation of gas production in unconventional reservoirs. In this study, we apply the Effective Medium Approximation, an upscaling technique from statistical physics, and modify the Doyen model for unconventional rocks. We develop an upscaling model to estimate the pore pressure-dependent gas permeability from pore throat size distribution, pore connectivity, tortuosity, porosity, and gas characteristics. We compare our adapted model with six data sets: three experiments, one pore-network model, and two lattice-Boltzmann simulations. Results showed that the proposed model estimated the gas permeability within a factor of 3 of the measurements/simulations in all data sets except the Eagle Ford experiment for which we discuss plausible sources of discrepancies.

The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism.

PubMed

Long, Lee Hua; Halliwell, Barry

2012-01-06

Several phenolic compounds as well as ascorbate can oxidise in certain cell culture media (especially Dulbecco's modified Eagle's medium (DMEM)) to generate hydrogen peroxide. Addition of oxaloacetate decreased the levels of H(2)O(2) detected and the oxaloacetate was depleted. Oxaloacetate was approximately as effective as pyruvate in decreasing H(2)O(2) levels and more effective than α-ketoglutarate. Our data raise important issues to consider when interpreting the behaviour and metabolism of cells in culture (which are both altered by the oxidative stress of cell culture) and their apparent response to addition of autooxidisable compounds such as ascorbate and epigallocatechin gallate. Copyright © 2011 Elsevier Inc. All rights reserved.

Preparation of non-aggregating aqueous fullerenes in highly saline solutions with a biocompatible non-ionic polymer

NASA Astrophysics Data System (ADS)

Aich, Nirupam; Boateng, Linkel K.; Flora, Joseph R. V.; Saleh, Navid B.

2013-10-01

Size-tunable stable aqueous fullerenes were prepared with different concentrations of biocompatible block-copolymer pluronic (PA) F-127, ranging from 0.001% to 1% (w/v). Size uniformity increased with the increase in PA concentration, yielding optimum 58.8 ± 5.6 and 61.8 ± 5.6 nm nC60s and nC70s, respectively (0.10%w/v PA), as observed using a dynamic light scattering technique. Fullerene aqueous suspensions also manifested enhanced stability in saline solution, Dulbecco’s modified Eagle medium (DMEM), and Roswell Park Memorial Institute (RPMI) culture medium. Transmission electron microscopy was performed to elaborate on the morphology and size specificity of fullerene clusters. Physicochemical characterizations of the suspended fullerenes were performed through UV-vis spectroscopy and electrophoretic mobility measurements. PA molecules showed size restriction by encasement, as observed via molecular dynamics simulations. Such solubilization with controllable size and non-aggregating behavior can facilitate application enhancement and mechanistic environmental and toxicological studies of size-specific fullerenes.

Calcium oxalate syntheses in a solution containing glucose by the atmospheric pressure plasma irradiation

NASA Astrophysics Data System (ADS)

Kurake, Naoyuki; Tanaka, Hiromasa; Ishikawa, Kenji; Nakamura, Kae; Kajiyama, Hiroaki; Kikkawa, Fumitaka; Mizuno, Masaaki; Yamanishi, Yoko; Hori, Masaru

2016-09-01

The non-equilibrium atmospheric pressure plasma (NEAPP) has been attracted attention because of its characteristic high reactivity even in a low temperature so that various phenomena by the NEAPP such as a sterilization, growth promotion and so forth have been reported around the world. Previously, we reported the NEAPP irradiation generated the calcium oxalate crystals in the medium, which contains 31 kinds of organics and inorganics. The Dulbecco's Modified Eagle Medium (DMEM) which was used in previous study is composed of no oxalate. Interestingly, not only crystallization but also synthesis of the oxalate was occurred by the NEAPP irradiation. Also the crystallization details were analyzed with the X-ray diffraction (XRD). In this study, we have clarified the mechanism on the crystallization due that D-glucose, calcium ion and bicarbonate ions are minimum essential components. The oxalate synthesis was proved by the gas chromatography and mass spectrometer (GC-MS). Finally, we conclude that a supersaturation of oxalic acid synthesized in those 3 species by the NEAPP.

A three-dimensional application with the numerical grid generation code: EAGLE (utilizing an externally generated surface)

NASA Technical Reports Server (NTRS)

Houston, Johnny L.

1990-01-01

Program EAGLE (Eglin Arbitrary Geometry Implicit Euler) is a multiblock grid generation and steady-state flow solver system. This system combines a boundary conforming surface generation, a composite block structure grid generation scheme, and a multiblock implicit Euler flow solver algorithm. The three codes are intended to be used sequentially from the definition of the configuration under study to the flow solution about the configuration. EAGLE was specifically designed to aid in the analysis of both freestream and interference flow field configurations. These configurations can be comprised of single or multiple bodies ranging from simple axisymmetric airframes to complex aircraft shapes with external weapons. Each body can be arbitrarily shaped with or without multiple lifting surfaces. Program EAGLE is written to compile and execute efficiently on any CRAY machine with or without Solid State Disk (SSD) devices. Also, the code uses namelist inputs which are supported by all CRAY machines using the FORTRAN Compiler CF177. The use of namelist inputs makes it easier for the user to understand the inputs and to operate Program EAGLE. Recently, the Code was modified to operate on other computers, especially the Sun Spare4 Workstation. Several two-dimensional grid configurations were completely and successfully developed using EAGLE. Currently, EAGLE is being used for three-dimension grid applications.

Eagle's syndrome: treatment by intraoral bilateral resection of the ossified stylohyoid ligament. A review and report of two cases.

PubMed

Martins, Wilson Denis; Ribas, Marina de Oliveira; Bisinelli, Julio; França, Beatriz Helena Sottile; Martins, Guilherme

2013-07-01

The complexities of Eagle's syndrome are examined according to anatomical, historical, clinical, and treatment aspects. There appears to be little correlation between the extent, form, and size of the anomalies of the styloid process and the stylohyoid ligament and the predictability of patients with related symptoms. Surgical treatment and the advantages and disadvantages of each surgical approach (intra- and extraoral) are discussed. The probable causes of enlargement of the styloid process and ossification of the stylohyoid ligament are addressed. Two cases of Eagle's syndrome are presented: one unilateral and the other bilateral. An intraoral modified surgical technique is presented.

Transformation of proanthocyanidin A2 to its isomers under different physiological pH conditions and common cell culture medium.

PubMed

Lu, Wen-Chien; Huang, Wei-Ting; Kumaran, Alaganandam; Ho, Chi-Tang; Hwang, Lucy Sun

2011-06-08

Proanthocyanidins constitute an important class of polyphenols ubiquitously found in plants. They have been extensively studied for their antioxidant capacity and bioactivity in vitro and in animal models. However, their stability under different pH conditions and in cell culture medium has not been well documented. In the present study, it was observed that proanthocyanidin A2 (PA2) was relatively more stable in acidic condition than in weak alkaline condition. PA2 was also quite unstable in basal-Dulbecco's Modified Eagle medium (b-DMEM medium) at 37 °C. The addition of PA2 to the cell culture medium accelerated its epimerization with a half-life of <15 min, and ethylenediaminetetraacetic acid (EDTA) could not stop the reaction. The results also demonstrated that the major isomers transformed in the weak alkaline condition or cell culture medium at 37 °C were identified as epicatechin-(4β→8; 2β→O→7)-ent-catechin (proanthocyanidin A4) and epicatechin-(4β→6; 2β→O→7)-ent-catechin. The rates of transformation were dependent on the pH or the components of the medium. Therefore, the results obtained for PA2 in the cell culture bioassays, which were usually carried out for 24 h, might not represent the true activity of the original PA2. The stability and transformation of PA2 should be considered when the bioactivity of PA2 is evaluated in a given cell culture system.

Stabilizing the cold plasma-stimulated medium by regulating medium’s composition

NASA Astrophysics Data System (ADS)

Yan, Dayun; Nourmohammadi, Niki; Bian, Ka; Murad, Ferid; Sherman, Jonathan H.; Keidar, Michael

2016-05-01

Over past several years, the cold plasma-stimulated medium (PSM) has shown its remarkable anti-cancer capacity in par with the direct cold plasma irradiation on cancer cells or tumor tissues. Independent of the cold plasma device, PSM has noticeable advantage of being a flexible platform in cancer treatment. Currently, the largest disadvantage of PSM is its degradation during the storage over a wide temperature range. So far, to stabilize PSM, it must be remained frozen at -80 °C. In this study, we first reveal that the degradation of PSM is mainly due to the reaction between the reactive species and specific amino acids; mainly cysteine and methionine in medium. Based on this finding, both H2O2 in PSM and the anti-cancer capacity of PSM can be significantly stabilized during the storage at 8 °C and -25 °C for at least 3 days by using phosphate-buffered saline (PBS) and cysteine/methionine-free Dulbecco’s Modified Eagle Medium (DMEM). In addition, we demonstrate that adding a tyrosine derivative, 3-Nitro-L-tyrosine, into DMEM can mitigate the degradation of PSM at 8 °C during 3 days of storage. This study provides a solid foundation for the future anti-cancer application of PSM.

Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model.

PubMed

Krawczyk, Krzysztof M; Matak, Damian; Szymanski, Lukasz; Szczylik, Cezary; Porta, Camillo; Czarnecka, Anna M

2018-04-01

The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial Institute Medium or Dulbecco's Modified Eagle's Medium and Complete Human Kidney Cancer Stem Cell Medium, respectively. Serum-free, animal-component free Human Embryonic Kidney 293 media were tested. Our results revealed that xeno-free embryonal renal cells optimized culture media provide a useful tool in RCC cancer biology research and at the same time enable effective growth of RCC. We propose bio-mimic RCC cell culture model with specific serum-free and xeno-free medium that promote RCC cell viability.

Establishment of a primary hepatocyte culture from the small Indian mongoose (Herpestes auropunctatus) and distribution of mercury in liver tissue.

PubMed

Horai, Sawako; Yanagi, Kumiko; Kaname, Tadashi; Yamamoto, Masatatsu; Watanabe, Izumi; Ogura, Go; Abe, Shintaro; Tanabe, Shinsuke; Furukawa, Tatsuhiko

2014-11-01

The present study established a primary hepatocyte culture for the small Indian mongoose (Herpestes auropunctatus). To determine the suitable medium for growing the primary hepatic cells of this species, we compared the condition of cells cultured in three media that are frequently used for mammalian cell culture: Dulbecco's Modified Eagle's Medium, RPMI-1640, and William's E. Of these, William's E medium was best suited for culturing the hepatic cells of this species. Using periodic acid-Schiff staining and ultrastructural observations, we demonstrated the cells collected from mongoose livers were hepatocytes. To evaluate the distribution of mercury (Hg) in the liver tissue, we carried out autometallography staining. Most of the Hg compounds were found in the central region of hepatic lobules. Smooth endoplasmic reticulum, which plays a role inxenobiotic metabolism, lipid/cholesterol metabolism, and the digestion and detoxification of lipophilic substances is grown in this area. This suggested that Hg colocalized with smooth endoplasmic reticulum. The results of the present study could be useful to identify the detoxification systems of wildlife with high Hg content in the body, and to evaluate the susceptibility of wildlife to Hg toxicity.

Combination of nutrients in a mammalian cell culture medium kills cryptococci.

PubMed

Granger, Donald L; Call, Donna M

2018-06-06

We found that a large inoculum of Cryptococcus gattii cells, when plated on Dulbecco's modified eagle's medium (DMEM) incorporated into agar, died within a few hours provided that DMEM agar plates had been stored in darkness for approximately 3 days after preparation. Standard conditions were developed for quantification of killing. The medium lost its fungicidal activity when exposed to visible light of wave length ∼400 nm. The amount of energy required was estimated at 5.8 × 104 joules @ 550 nm. Liquid DMEM conditioned by incubation over DMEM agar plates stored in darkness was fungicidal. We found that fungicidal activity was heat-stable (100°C). Dialysis tubing with MWC0 < 100 Daltons retained fungicidal activity. Neutral pH was required. Strains of Cryptococcus were uniformly sensitive, but some Candida species were resistant. Components of DMEM required for killing were pyridoxal and cystine. Micromolar amounts of iron shortened the time required for DMEM agar plates to become fungicidal when stored in the dark. Organic and inorganic compounds bearing reduced sulfur atoms at millimolar concentrations inhibited fungicidal activity. Our results point to a light-sensitive antifungal compound formed by reaction of pyridoxal with cystine possibly by Schiff base formation.

Medium Calcium Concentration Determines Keratin Intermediate Filament Density and Distribution in Immortalized Cultured Thymic Epithelial Cells (TECs)

NASA Astrophysics Data System (ADS)

Sands, Sandra S.; Meek, William D.; Hayashi, Jun; Ketchum, Robert J.

2005-08-01

Isolation and culture of thymic epithelial cells (TECs) using conventional primary tissue culture techniques under conditions employing supplemented low calcium medium yielded an immortalized cell line derived from the LDA rat (Lewis [Rt1l] cross DA [Rt1a]) that could be manipulated in vitro. Thymi were harvested from 4 5-day-old neonates, enzymically digested using collagenase (1 mg/ml, 37°C, 1 h) and cultured in low calcium WAJC404A medium containing cholera toxin (20 ng/ml), dexamethasone (10 nM), epidermal growth factor (10 ng/ml), insulin (10 [mu]g/ml), transferrin (10 [mu]g/ml), 2% calf serum, 2.5% Dulbecco's Modified Eagle's Medium (DMEM), and 1% antibiotic/antimycotic. TECs cultured in low calcium displayed round to spindle-shaped morphology, distinct intercellular spaces (even at confluence), and dense reticular-like keratin patterns. In high calcium (0.188 mM), TECs formed cobblestone-like confluent monolayers that were resistant to trypsinization (0.05%) and displayed keratin intermediate filaments concentrated at desmosomal junctions between contiguous cells. Changes in cultured TEC morphology were quantified by an analysis of desmosome/membrane relationships in high and low calcium media. Desmosomes were significantly increased in the high calcium medium. These studies may have value when considering the growth conditions of cultured primary cell lines like TECs.

Evaluation of the potential toxicity of unmodified and modified cyclodextrins on murine blood-brain barrier endothelial cells.

PubMed

Shityakov, Sergey; Salmas, Ramin Ekhteiari; Salvador, Ellaine; Roewer, Norbert; Broscheit, Jens; Förster, Carola

2016-04-01

In this study, we investigated the cytotoxic effects of unmodified α-cyclodextrin (α-CD) and modified cyclodextrins, including trimethyl-β-cyclodextrin (TRIMEB) and hydroxypropyl-β-cyclodextrin (HPβCD), on immortalized murine microvascular endothelial (cEND) cells of the blood-brain barrier (BBB). A CellTiter-Glo viability test, performed on the cEND cells showed significant differences among the different cyclodextrins. After 24 hr of incubation, TRIMEB was the most cytotoxic, and HPβCD was non-toxic. α-CD and TRIMEB exhibited greater cytotoxicity in the Dulbecco's modified Eagle's medium than in heat-inactivated human serum indicating protective properties of the human serum. The predicted dynamic toxicity profiles (Td) for α-CD and TRIMEB indicated higher cytotoxicity for these cyclodextrins compared to the reference compound (dimethylsulfoxide). Molecular dynamics simulation of cholesterol binding to the CDs suggested that not just cholesterol but phospholipids extraction might be involved in the cytotoxicity. Overall, the results demonstrate that HPβCD has the potential to be used as a candidate for drug delivery vector development and signify a correlation between the in vitro cytotoxic effect and cholesterol binding of cyclodextrins.

Catalase protection of neuronal survival in vitro is not directed to the accumulation of peroxides in the culture medium.

PubMed

Martin, E M; Skaper, S D; Varon, S

1987-01-01

Walicke et al. (1986, J. Neurosci. 6, 1114-1121) have shown that catalase can replace the pyruvate requirement for survival of CNS neurons cultured in vitro. Since presently the only known function of catalase is the enzymatic degradation of hydrogen peroxide to water and oxygen, the simplest interpretation of the ability of catalase to support neuronal survival would be that catalase removes from the culture medium hydrogen peroxide. To test this hypothesis 8-day embryonic chick forebrain cells were cultured for 24 hr in a modified Eagle's Basal Medium with the serum-free supplement N1 (HEBM/N1) in the presence or absence of Phenol Red, 20 micrograms/ml catalase, 1 mM pyruvate, and/or 25 mM N-2-hydroxyethylpiperazine-N'-2-ethane-sulfonic acid (HEPES) on a polyornithine-laminin substratum. The various media were then assayed for peroxide content using the potassium iodide method described by Wang and Nixon (1978, In Vitro 14, 714-722). The present data reveal that (1) HEBM/N1 normally contains approximately 50 microM peroxides, little of which is hydrogen peroxide, (2) the organic peroxide levels accumulating in this medium are not reduced by either catalase or pyruvate, and (3) medium modifications can reduce to no longer detectable levels the peroxides accumulating in the medium, but catalase or pyruvate is still required for neuronal survival. We conclude that catalase must exert its survival-promoting action at levels other than peroxides accumulating in the culture medium.

Non-covalent interaction between dietary stilbenoids and human serum albumin: Structure-affinity relationship, and its influence on the stability, free radical scavenging activity and cell uptake of stilbenoids.

PubMed

Cao, Hui; Jia, Xueping; Shi, Jian; Xiao, Jianbo; Chen, Xiaoqing

2016-07-01

Dietary stilbenoids are associated with many benefits for human health, which depend on their bioavailability and bioaccessibility. The stilbenoid-human serum albumin (HSA) interactions are investigated to explore the structure-affinity relationship and influence on the stability, free radical scavenging activity and cell uptake of stilbenoids. The structure-affinity relationship of the stilbenoids-HSA interaction was found as: (1) the methoxylation enhanced the affinity, (2) an additional hydroxyl group increases the affinity and (3) the glycosylation significantly weakened the affinity. HSA obviously masked the free radical scavenging potential of stilbenoids. The stabilities of stilbenoids in different medium were determined as: HSA solution>human plasma>Dulbecco's modified Eagle's medium. It appears that the milk enhanced the cell uptake of stilbenoids with multi-hydroxyl groups and weakened the cell uptake of stilbenoids with methoxyl group on EA.hy 926 endothelial cells. The stilbenoids are hardly absorbed by human umbilical vein endothelial cells in the presence of milk. Copyright © 2016 Elsevier Ltd. All rights reserved.

The utility of point count surveys to predict wildlife interactions with wind energy facilities: An example focused on golden eagles

USGS Publications Warehouse

Sur, Maitreyi; Belthoff, James R.; Bjerre, Emily R.; Millsap, Brian A.; Katzner, Todd

2018-01-01

Wind energy development is rapidly expanding in North America, often accompanied by requirements to survey potential facility locations for existing wildlife. Within the USA, golden eagles (Aquila chrysaetos) are among the most high-profile species of birds that are at risk from wind turbines. To minimize golden eagle fatalities in areas proposed for wind development, modified point count surveys are usually conducted to estimate use by these birds. However, it is not always clear what drives variation in the relationship between on-site point count data and actual use by eagles of a wind energy project footprint. We used existing GPS-GSM telemetry data, collected at 15 min intervals from 13 golden eagles in 2012 and 2013, to explore the relationship between point count data and eagle use of an entire project footprint. To do this, we overlaid the telemetry data on hypothetical project footprints and simulated a variety of point count sampling strategies for those footprints. We compared the time an eagle was found in the sample plots with the time it was found in the project footprint using a metric we called “error due to sampling”. Error due to sampling for individual eagles appeared to be influenced by interactions between the size of the project footprint (20, 40, 90 or 180 km2) and the sampling type (random, systematic or stratified) and was greatest on 90 km2 plots. However, use of random sampling resulted in lowest error due to sampling within intermediate sized plots. In addition sampling intensity and sampling frequency both influenced the effectiveness of point count sampling. Although our work focuses on individual eagles (not the eagle populations typically surveyed in the field), our analysis shows both the utility of simulations to identify specific influences on error and also potential improvements to sampling that consider the context-specific manner that point counts are laid out on the landscape.

Effect of selenium on malignant tumor cells of brain.

PubMed

Zhu, Z; Kimura, M; Itokawa, Y; Nakatsu, S; Oda, Y; Kikuchi, H

1995-07-01

Some reports have demonstrated that selenium can inhibit tumorigenesis in some tissues of animal. However, little is known about the inhibitory effect on malignant tumor cells of brain. The purpose of our study was to determine the biological effect of selenium on growth of rat glioma and human glioblastoma cell lines. Cell lines C6 and A172 were obtained from Japanese Cancer Research Resources Bank, Tokyo, Japan (JCRB). Cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum at 37 degrees C in a humidified atmosphere of air and 5% CO2. Antiproliferative effects of selenium were evaluated using growth rate assay quantifying cell number by MTT assay. An antiproliferative effect of selenium was found in two cell lines, which was more effective on human A172 glioblastoma and less effective on rat C6 glioma.

Soy milk as a storage medium to preserve human fibroblast cell viability: an in vitro study.

PubMed

Moura, Camilla Christian Gomes; Soares, Priscilla Barbosa Ferreira; Reis, Manuella Verdinelli de Paula; Fernandes Neto, Alfredo Júlio; Soares, Carlos José

2012-01-01

Soy milk (SM) is widely consumed worldwide as a substitute for cow milk. It is a source of vitamins, carbohydrates and sugars, but its capacity to preserve cell viability has not been evaluated. The purpose of the present study was to investigate the efficacy of SM to maintain the viability of human fibroblasts at short periods compared with different cow milks. Human mouth fibroblasts were cultured and stored in the following media at room temperature: 10% Dulbecco's Modified Eagle Medium (DMEM) (positive control group); long shelf-life ultra-high temperature whole cow milk (WM); long shelf-life ultra-high temperature skim cow milk (SKM); powdered cow milk (PM); and soy milk (SM). After 5, 15, 30 and 45 min, cell viability was analyzed using the MTT assay. Data were analyzed statistically by the Kruskal-Wallis test with post-analysis using the Dunn's method (α=0.05). SKM showed the lowest capacity to maintain cell viability in all analyzed times (p<0.05). At 30 and 45 min, the absorbance levels in control group (DMEM) and SM were significantly higher than in SKM (p<0.05). Cell viability decreased along the time (5-45 min). The results indicate that SM can be used as a more adequate storage medium for avulsed teeth. SKM was not as effective in preserving cell viability as the cell culture medium and SM.

Eagle's syndrome associated with lingual nerve paresthesia: a case report.

PubMed

Dong, Zhiwei; Bao, Haihong; Zhang, Li; Hua, Zequan

2014-05-01

Eagle's syndrome is characterized by a variety of symptoms, including throat pain, sensation of a foreign body in the pharynx, dysphagia, referred otalgia, and neck and throat pain exacerbated by head rotation. Any styloid process longer than 25 mm should be considered elongated and will usually be responsible for Eagle's syndrome. Surgical resection of the elongated styloid is a routine treatment and can be accomplished using a transoral or an extraoral approach. We report a patient with a rare giant styloid process that was approximately 81.7 mm. He complained of a rare symptom: hemitongue paresthesia. After removal of the elongated styloid process using the extraoral approach, his symptoms, including the hemitongue paresthesia, were alleviated. We concluded that if the styloid process displays medium to severe elongation, the extraoral approach will be appropriate. Copyright © 2014 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

The effect of virtual cross linking on the oxidative stability and lipid uptake of aliphatic poly(urethane urea).

PubMed

Thomas, Vinoy; Jayabalan, Muthu

2002-01-01

In vitro oxidative degradation and lipid sorption of aliphatic, low elastic modulus and virtually cross-linked poly(urethane urea)s based on 4,4' methylene bis(cyclohexyl isocyanate), hydroxy terminated poly butadiene and hexamethylene diamine were evaluated. The aged samples revealed no weight loss in the oxidation medium. The IR spectral analyses revealed the stability of unsaturated double bonds at 964 cm(-1) (characteristic for polybutadiene soft segment) with no change in peak intensity. The poly(tetramethylene glycol) (PTMG)-added poly(ether urethane urea) polymer also revealed no disappearance of IR peaks for ether and unsaturated double bonds in samples aged in vitro oxidation medium. All the polymers have shown increase in weight due to lipid up take in lipid-rich medium (palm oil) but it was rather low in Dulbecco's modified eagle medium (DMEM) cholesterol. The slight change in mechanical properties of the present polymers in oxidation and DMEM is due to the rearrangement of molecular structure with virtual cross links of hydrogen bonding (physical cross linking) without degradation and plasticization effect of lipid. The influence of these media on the rearrangement of virtual cross links has been observed. Higher the virtual cross-link density, lesser is the loss of tensile properties of poly(urethane urea)s in the oxidation medium and vice versa. On the other hand, higher the virtual cross-link density of poly(urethane urea), higher is the loss of ultimate tensile strength and stress at 100% strain and vice versa in DMEM medium.

Chromosome reshuffling in birds of prey: the karyotype of the world's largest eagle (Harpy eagle, Harpia harpyja) compared to that of the chicken (Gallus gallus).

PubMed

de Oliveira, Edivaldo H C; Habermann, Felix A; Lacerda, Oneida; Sbalqueiro, Ives J; Wienberg, Johannes; Müller, Stefan

2005-11-01

Like various other diurnal birds of prey, the world's largest eagle, the Harpy (Harpia harpyja), presents an atypical bird karyotype with 2n=58 chromosomes. There is little knowledge about the dramatic changes in the genomic reorganization of these species compared to other birds. Since recently, the chicken provides a "default map" for various birds including the first genomic DNA sequence of a bird species. Obviously, the gross division of the chicken genome into relatively gene-poor macrochromosomes and predominantly gene-rich microchromosomes has been conserved for more than 150 million years in most bird species. Here, we present classical features of the Harpy eagle karyotype but also chromosomal homologies between H. harpyja and the chicken by chromosome painting and comparison to the chicken genome map. We used two different sets of painting probes: (1) chicken chromosomes were divided into three size categories: (a) macrochromosomes 1-5 and Z, (b) medium-sized chromosomes 6-10, and (c) 19 microchromosomes; (2) combinatorially labeled chicken chromosome paints 1-6 and Z. Both probe sets were visualized on H. harpyja chromosomes by multicolor fluorescence in situ hybridization (FISH). Our data show how the organization into micro- and macrochromosomes has been lost in the Harpy eagle, seemingly without any preference or constraints.

A simple and effective protocol for fast isolation of human Tenon's fibroblasts from a single trabeculectomy biopsy - a comparison of cell behaviour in different culture media.

PubMed

Przekora, Agata; Zarnowski, Tomasz; Ginalska, Grazyna

2017-01-01

Human Tenon's fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an effective and fast protocol for HTF isolation from trabeculectomy biopsies. The protocol was compared with the commonly recommended HTF isolation procedure, which uses Dulbecco's modified Eagle's medium (DMEM). We used Eagle's minimum essential medium (EMEM) enriched with fibroblast growth factor (FGF), which selectively promoted the proliferation of HTF cells. A secondary goal was to compare HTF morphology, metabolism and growth during parallel cultivation of the isolated cells in FGF-enriched EMEM and DMEM. Standard procedures for HTF isolation from tissue biopsies require a 20- to 30-day culture of the explants to obtain the first monolayer. Our protocol yielded the first monolayer after approx. 15 days. More importantly, the majority of the cells were fibroblasts with only individual epithelium-derived cells present. Using FGF-enriched EMEM allowed 1.3 × 10 6 vimentin-positive fibroblasts to be obtained from a single biopsy within approx. 25 days. Using DMEM resulted in isolation failure and required exchange to FGF-enriched medium to recover the fibroblast culture. HTFs maintained in FGF-enriched EMEM also showed faster proliferation and a different type I collagen production ability compared to HTFs cultured in DMEM. Thus, FGF-enriched EMEM is recommended for fast propagation of HTFs unless the aim of the study is to assess the effect of a tested agent on proliferation ability or type I collagen production. Our fast protocol for HTF isolation allows easy setup of cell banks by researchers under laboratory conditions and could be very useful during testing of novel ophthalmologic anti-fibrotic agents in vitro. Molecular analysis of HTFs isolated from patients with known treatment histories may provide valuable information on the effects of some medications taken before glaucoma surgery on the subsequent wound-healing process and potential for trabeculectomy failure.

In vitro thermal effects on embryonic cells of endangered hawksbill turtle Eretmochelys imbricata.

PubMed

Takeshita, Satoshi; Matsuda, Naoki; Kodama, Seiji; Suzuki, Keiji; Watanabe, Masami

2013-12-01

The hawksbill turtle is an ectotherm, whose sex is determined by temperature during embryonic development. This study aimed to determine whether embryonic hawksbill turtle cells respond differently to temperature than mammalian cells. Embryonic hawksbill turtle cells were established in culture, and thermal effects on these cells were investigated in vitro. Cells were maintained in Dulbecco's Modified Eagle Medium supplemented with non-essential amino acids, vitamin solution, sodium pyruvate, and 10% fetal bovine serum at 33°C and cell proliferation occurred at 25-33°C. When cells were incubated at 37°C (the temperature of mammalian cell culture) for 24 h, cell growth was completely inhibited. This growth inhibition was evidently recovered by changing the incubation temperature back to 33°C. Expression of heat shock protein was found to increase with elevating culture temperature from 25 to 33°C.

Synthesis, characterization, bioactivity and antibacterial studies of silver doped calcium borosilicate glass-ceramics

NASA Astrophysics Data System (ADS)

Kumar, Alesh; Mariappan, C. R.

2018-04-01

Bioactive glass-ceramics 45.8 mol% SiO- 45.8 CaO - 8.4 B2O3 doped with Ag2O were synthesized by sol-gel method. The glass-ceramic nature of samples was confirmed by X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis. Fourier transform infrared (FT-IR) spectra reveal the probable stretching and bending vibration modes of silicate and borate groups. UV-Visible spectra reveal the presence of Ag+ ions and metallic Ag in the glass matrix for Ag2O doped ceramic sample. Biocompatibility of the glass nature of samples was studied by soaking of samples in Dulbecco's Modified Eagle's Medium (DMEM) with subsequent XRD studies. It was found that bone-like apatite formation on the glasses after soaked in DMEM. Antibacterial studies of glass ceramics powder against gram positive and negative microorganisms were carried out.

In vitro generation of type-II pneumocytes can be initiated in human CD34(+) stem cells.

PubMed

Srikanth, Lokanathan; Venkatesh, Katari; Sunitha, Manne Mudhu; Kumar, Pasupuleti Santhosh; Chandrasekhar, Chodimella; Vengamma, Bhuma; Sarma, Potukuchi Venkata Gurunadha Krishna

2016-02-01

Human CD34(+) stem cells differentiated into type-II pneumocytes in Dulbecco's modified Eagle medium (DMEM) having hydrocortisone, insulin, fibroblast growth factor (FGF), epidermal growth factor (EGF) and bovine serum albumin (BSA), expressing surfactant proteins-B (SP-B) and C (SP-C), alkaline phosphatase (ALP) and lysozyme. FACS-enumerated pure CD34(+) cells, isolated from human peripheral blood, were cultured in DMEM and showed positive reaction with anti-human CD34 monoclonal antibodies in immunocytochemistry. These cells were cultured in DMEM having hydrocortisone, insulin, FGF, EGF and BSA (HIFEB-D) medium having an air-liquid interface. They differentiated into type-II pneumocytes with expression of SP-B and SP-C genes and disappearance of CD34 expression as assessed using real-time PCR. In reverse transcription-PCR amplicons showed 208 and 907 bp confirming SP-B and SP-C expressions. These cells expressed ALP with an activity of 1.05 ± 0.09 mM ml(-1) min(-1) and lysozyme that killed E. coli. The successful differentiation of human CD34(+) stem cells into type-II pneumocytes, and transplantation of such cells obtained from the patient's stem cell could be the futuristic approach to regenerate diseased lung alveoli.

Magnesium degradation influenced by buffering salts in concentrations typical of in vitro and in vivo models.

PubMed

Agha, Nezha Ahmad; Feyerabend, Frank; Mihailova, Boriana; Heidrich, Stefanie; Bismayer, Ulrich; Willumeit-Römer, Regine

2016-01-01

Magnesium and its alloys have considerable potential for orthopedic applications. During the degradation process the interface between material and tissue is continuously changing. Moreover, too fast or uncontrolled degradation is detrimental for the outcome in vivo. Therefore in vitro setups utilizing physiological conditions are promising for the material/degradation analysis prior to animal experiments. The aim of this study is to elucidate the influence of inorganic salts contributing to the blood buffering capacity on degradation. Extruded pure magnesium samples were immersed under cell culture conditions for 3 and 10 days. Hank's balanced salt solution without calcium and magnesium (HBSS) plus 10% of fetal bovine serum (FBS) was used as the basic immersion medium. Additionally, different inorganic salts were added with respect to concentration in Dulbecco's modified Eagle's medium (DMEM, in vitro model) and human plasma (in vivo model) to form 12 different immersion media. Influences on the surrounding environment were observed by measuring pH and osmolality. The degradation interface was analyzed by electron-induced X-ray emission (EIXE) spectroscopy, including chemical-element mappings and electron microprobe analysis, as well as Fourier transform infrared reflection micro-spectroscopy (FTIR). Copyright © 2015 Elsevier B.V. All rights reserved.

Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells.

PubMed

Arslan, Belkis Atasever; Isik, Fatma Busra; Gur, Hazal; Ozen, Fatih; Catal, Tunc

2017-10-01

Nigella sativa is from botanical Ranunculaceae family and commonly known as black seed. Apoptotic effect of N. sativa and its apoptotic signaling pathways on U937 lymphoma cells are unknown. In this study, we investigated selective cytotoxic and apoptotic effects of N. sativa extract and its apoptotic mechanisms on U937 cells. In addition, we also studied selective cytotoxic activity of thymoquinone that is the most active essential oil of N. sativa . Our results showed that N. sativa extract has selective cytotoxicity and apoptotic effects on U937 cells but not ECV304 control cells. However, thymoquinone had no significant cytotoxicity against on both cells. N. sativa extract increased significantly caspase-3, BAD, and p53 gene expressions in U937 cells. N. sativa may have anticancer drug potential and trigger p53-induced apoptosis in U937 lymphoma cells. This is the first study showing the apoptotic effect of Nigella sativa extract on U937 cells. Abbreviations used: CI: Cytotoxicity index, DMEM: Dulbecco's Modified Eagle Medium, HL: Hodgkin's lymphoma, MTT: 3-(4,5-dimethy lthiazol-2yl)-2,5-diphenyl tetrazolium bromide, RPMI: Roswell Park Memorial Institute medium.

Increasing efficiency of human mesenchymal stromal cell culture by optimization of microcarrier concentration and design of medium feed.

PubMed

Chen, Allen Kuan-Liang; Chew, Yi Kong; Tan, Hong Yu; Reuveny, Shaul; Weng Oh, Steve Kah

2015-02-01

Large amounts of human mesenchymal stromal cells (MSCs) are needed for clinical cellular therapy. In a previous publication, we described a microcarrier-based process for expansion of MSCs. The present study optimized this process by selecting suitable basal media, microcarrier concentration and feeding regime to achieve higher cell yields and more efficient medium utilization. MSCs were expanded in stirred cultures on Cytodex 3 microcarriers with media containing 10% fetal bovine serum. Process optimization was carried out in spinner flasks. A 2-L bioreactor with an automated feeding system was used to validate the optimized parameters explored in spinner flask cultures. Minimum essential medium-α-based medium supported faster MSC growth on microcarriers than did Dulbecco's modified Eagle's medium (doubling time, 31.6 ± 1.4 vs 42 ± 1.7 h) and shortened the process time. At microcarrier concentration of 8 mg/mL, a high cell concentration of 1.08 × 10(6) cells/mL with confluent cell concentration of 4.7 × 10(4)cells/cm(2) was achieved. Instead of 50% medium exchange every 2 days, we have designed a full medium feed that is based on glucose consumption rate. The optimal medium feed that consisted of 1.5 g/L glucose supported MSC growth to full confluency while achieving the low medium usage efficiency of 3.29 mL/10(6)cells. Finally, a controlled bioreactor with the optimized parameters achieved maximal confluent cell concentration with 16-fold expansion and a further improved medium usage efficiency of 1.68 mL/10(6)cells. We have optimized the microcarrier-based platform for expansion of MSCs that generated high cell yields in a more efficient and cost-effective manner. This study highlighted the critical parameters in the optimization of MSC production process. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

PubMed

Sidney, Laura E; Branch, Matthew J; Dua, Harminder S; Hopkinson, Andrew

2015-12-01

The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Primary human corneal stroma-derived stem cells (CSSCs) were extracted from corneoscleral rims. Culture in seven different media types was compared: Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS); M199 with 20% FBS; DMEM-F12 with 20% serum replacement, basic fibroblast growth factor and leukemia inhibitory factor (SCM); endothelial growth medium (EGM); semi-solid MethoCult; serum-free keratinocyte medium (K-SFM); and StemPro-34. Effects on proliferation, morphology, protein and messenger RNA expression were evaluated. All media supported proliferation of CSSCs with the exception of K-SFM and StemPro-34. Morphology differed between media: DMEM produced large cells, whereas EGM produced very small cells. Culture in M199 produced a typical mesenchymal stromal cell phenotype with high expression of CD105, CD90 and CD73 but not CD34. Culture in SCM produced a phenotype more reminiscent of a progenitor cell type with expression of CD34, ABCG2, SSEA-4 and PAX6. Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we consider it to be the most appropriate for development as a clinical-grade medium for the production of CSSC phenotypes suitable for cell therapy. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

[Marrow stromal cells cultured in N2-supplemented medium: implications on the generation of neural cells].

PubMed

Castillo-Díaz, L; de la Cuétara-Bernal, K; García-Varona, A Y

Most of the culture system for in vitro maintenance and neural differentiation of marrow stromal cells (MSCs) use synthetic media supplemented with 10 or 20% fetal bovine serum (FBS). Serum, however, is comprised of unknown quantities of undefined substances which could interfere the effect of exogenous substances on neural differentiation of MSCs. AIM. Here we describe survival of MSCs cultured in culture conditions where serum was reduced at 0.5 and 1% using Bottenstein and Sato's N2 formula (1979) and poly-L-lysine (PLL)-coated substrate. Stromal cells isolated from rat femurs were cultivated in Dulbecco's modified Eagle medium at 10, 1, 0.5% FBS or in serum free medium containing N2 formula. In serum free medium or at low serum concentration culture surface was coated with PLL. Cell survival was determined by MTT method or by counting viable cells. Survival of MSCs cultured in N2 supplement was reduced at about 40% of that observed in 10% FBS containing medium. Under these conditions cell morphology was also affected. When N2 containing medium was supplemented with FBS at 0.5 or 1% a significant increase of survival with respect to that observed in N2-supplemented cultures was observed. Cells seeded on PLL-coated surface increased their survival by contrast with their homologous cultures seeded on uncoated surface. The culture system which combines N2 formula with FBS 1% and PLL-coated surface is useful for the maintenance of MSCs. These conditions offer advantages for the study of differentiation of these cells because they reduce the confounding influence of serum. The possible implication of this culture system for the study of neural differentiation by these cells is discussed.

Essential components for ex vivo proliferation of mesenchymal stromal cells.

PubMed

Fekete, Natalie; Rojewski, Markus Thomas; Lotfi, Ramin; Schrezenmeier, Hubert

2014-02-01

Mesenchymal stromal cells (MSCs) are highly interesting candidates for clinical applications in regenerative medicine. Due to their low occurrence in human tissues, extensive in vitro expansion is necessary to obtain sufficient cell numbers applicable as a clinical dose in the context of cellular therapy. Current cell culture media formulations for the isolation and expansion of MSCs include fetal calf serum (FCS), human AB serum (ABS), or human platelet lysate (PL) as a supplement. However, these established supplements are inherently ill-defined formulations that contain a variety of bioactive molecules in varying batch-to-batch compositions and the risk of transmitting pathogens that escape routine screening procedures. In this study, we have comparatively characterized the capacity of commonly used basal media, such as the Minimum Essential Medium alpha (αMEM), Dulbecco's modified Eagle's medium (DMEM), Iscove's Modified Dulbecco's Medium (IMDM), and RPMI 1640 as well as human- and animal-derived supplements, that is, PL, ABS, and FCS to stimulate cell proliferation. MSC proliferation was observed to be optimal in the PL-supplemented αMEM. Using a combinatorial approach, we then assessed a library of soluble factors, including mitogens (TGF-β1, Activin A, bFGF, EGF, IGF-I, PDGF-BB, and VEGF), chemokines (CCL21, CCL25, CXCL12, and RANTES), proteins (human serum albumin), lipids (e.g., oleic acid, linoleic acid, and arachidonic acid), and hormones (dexamethasone, insulin, and TSH), to create a defined medium as well as coating of cell culture surfaces to promote robust MSC proliferation in vitro. A combination of recombinant human factors partially met the nutritional requirements of bone marrow-derived MSCs, and was able to promote cell proliferation comparable to about 5% PL if supplemented with auxiliary 0.6%-1.2% PL. Maximal MSC proliferation was achieved by combining 5% PL with a cocktail of recombinant factors and did not depend on coating of cell culture surfaces.

Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment.

PubMed

Zhang, G; Lu, S; Chen, T A; Funk, C R; Meyer, W A

2003-06-01

A transformation system for triploid bermudagrass ( Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 microM dicamba, 20 microM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase ( hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.

Osteogenic differentiation of immature osteoblasts: Interplay of cell culture media and supplements.

PubMed

Brauer, A; Pohlemann, T; Metzger, W

2016-01-01

Differentiation of immature osteoblasts to mature osteoblasts in vitro initially was induced by supplementing the medium with β-gylcerophosphate and dexamethasone. Later, ascorbic acid, vitamin D3, vitamin K3 and TGFβ1 were used in varying concentrations as supplements to generate a mature osteoblast phenotype. We tested the effects of several combinations of cell culture media, seeding protocols and osteogenic supplements on osteogenic differentiation of human primary osteoblasts. Osteogenic differentiation was analyzed by staining alkaline phosphatase (ALP) with 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT) and by von Kossa staining of deposited calcium phosphate. The combinations of culture media and supplements significantly influenced osteogenic differentiation, but the seeding protocol did not. Staining of ALP and calcium phosphate could be achieved only if our own mix of osteogenic supplements was used in combination with Dulbecco's modified Eagle medium or if a commercial mix of osteogenic supplements was used in combination with osteoblast growth medium. Especially for von Kossa, we observed great variations in the staining intensity. Because osteogenic differentiation is a complex process, the origin of the osteoblasts, cell culture media and osteogenic supplements should be established by preliminary experiments to achieve optimal differentiation. Staining of ALP or deposited calcium phosphate should be supplemented with qRT-PCR studies to learn more about the influence of specific supplements on osteogenic markers.

Establishment and characterization of a new marine fish cell line from ovary of barfin flounder ( Verasper moseri)

NASA Astrophysics Data System (ADS)

Xu, Xiaohui; Fan, Tingjun; Jiang, Guojian; Yang, Xiuxia

2015-12-01

A novel continuous ovary cell line from barfin flounder ( Verasper moseri) (BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22°C in Dulbecco's modified Eagle medium/F12 medium (DMEM/F12, 1:1; pH 7.2) supplemented with 20% fetal bovine serum (FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22°C. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number (46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein (GFP) positively expressed in these cells after being transformed with pcDNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish.

In vivo imaging of brain infarct with the novel fluorescent probe PSVue 794 in a rat middle cerebral artery occlusion-reperfusion model.

PubMed

Chu, Chun; Huang, Xiaofang; Chen, Chiung-Tong; Zhao, Yuanli; Luo, Jin J; Gray, Brian D; Pak, Koon Y; Dun, Nae J

2013-01-01

The utility of PSVue 794 (PS794), a near-infrared fluorescent dye conjugated to a bis[zinc (II)-dipicolylamine] (Zn-DPA) targeting moiety, in imaging brain infarct was assessed in a rat middle cerebral artery occlusion-reperfusion model. Following reperfusion, 1 mM PS794 solution was administered intravenously via a tail vein. Fluorescence images were captured between 6 to 72 hours postinjection using a LI-COR Biosciences Pearl Imaging System. Strong fluorescence signals, which may represent the infarct core, were detected in the right hemisphere, ipsilateral to the injured site, and weaker signals in areas surrounding the core. In ischemia-reperfusion rats injected with a control dye not linked to a targeting agent, fluorescence was distributed diffusely throughout the brain. To address the issue of whether Zn-DPA targets apoptotic/necrotic cells, HT22 mouse hippocampal neurons were cultured in either Dulbecco's Modified Eagle's Medium, serum-deprived medium, Hank's Balanced Salt Solution, or L-glutamate (10 mM)-containing medium for up to 33 hours. Cells were then double-labeled with PSVue 480 (Zn-DPA conjugated to fluorescein isothiocyanate) and propidium iodide, which labels necrotic cells. Microscopic examination revealed that PS480 targeted apoptotic and necrotic cells. The result indicates that PS794 is applicable to in vivo imaging of brain infarct and that Zn-DPA selectively targets apoptotic/necrotic cells.

Rates of in vivo (arterial) and in vitro biocorrosion for pure magnesium.

PubMed

Bowen, Patrick K; Drelich, Adam; Drelich, Jaroslaw; Goldman, Jeremy

2015-01-01

The development of magnesium-based materials for bioabsorbable stents relies heavily on corrosion testing by immersion in pseudophysiological solutions, where magnesium degrades faster than it does in vivo. The quantitative difference in corrosion kinetics in vitro and in vivo is largely unknown, but, if determined, would help reduce dependence on animal models. In order to create a quantitative in vitro-in vivo correlation based on an accepted measure of corrosion (penetration rate), commercially pure magnesium wires were corroded in vivo in the abdominal aortas of rats for 5-32 days, and in vitro for up to 14 days using Dulbecco's modified eagle medium. Cross-sectioning, scanning electron microscopy, image analysis, a modified penetration rate tailored to degraded wires, and empirical modeling were used to analyze the corroded specimens. In vitro penetration rates were consistently higher than comparable in vivo rates by a factor of 1.2-1.9× (±0.2×). For a sample <20% corroded, an approximate in vitro-in vivo multiplier of 1.3 ± 0.2× was applied, whereas a multiplier of 1.8 ± 0.2× became appropriate when the magnesium specimen was 25-35% degraded. © 2014 Wiley Periodicals, Inc.

The release of elements from dental casting alloy into cell-culture medium and artificial saliva.

PubMed

Can, Gülşen; Akpınar, Gül; Aydın, Ahmet

2007-04-01

The biocompatibility of dental casting alloys is a critical issue because these alloys are in long-term intimate contact with oral tissues. Since the biocompatibility of alloys is not completely known; the release of elements from the alloys has been studied. The aim of this study was to compare the elemental release from dental casting alloy during exposure to artificial saliva and cell-culture medium. Twenty specimens made from Ni-Cr alloy were provided in the form of 5 mm diameter discs, 2 mm in thickness with a 7 mm stem attached to one face to facilitate handling. Ten of twenty samples were polished separately using a conventional technique. The remaining ten samples were left sandblasted with 50 mum Al(2)0(3). Ten samples (5 polished, 5 sandblasted) were separately placed into cell-culture wells with Dulbecco's Modified Eagle's Medium. The other ten samples were placed separately into cell-culture wells with artificial saliva. The samples were subjected in contact with these medium for 30 days. These medium were collected every 7 days. The cell-culture medium and artificial saliva without alloy samples were subjected to elemental analyses as a control. At the end of the exposure time, Atomic Absorption Spectrometry (AAS) was used to determine the release of elements from the alloys into all collected medium. Statistical analyses were assessed with two-way ANOVA. In general, the elemental release occurred with in all medium. The elemental releases of sandblasted alloys were higher than polished alloys. Artificial saliva was found to cause more release from the samples. In both media, Ni released from polished and sandblasted alloys were higher than Cr and Mo. The results suggest that the release of elements from the alloys might have correlated with the environments and the surface of dental alloy.

Correlation of Immunomodulatory and Therapeutic Activities of Interferon and Interferon Inducers in Metastatic Disease

DTIC Science & Technology

1988-01-01

Eagle’s minimum essential medium with Earle’s salts, supplemented with 5% fetal bovine serum, L-glutamine, sodium pyruvate, nonessential amino acids...LC (iv., tiw) was initiated 1 day lacer . The statistical significance of the differences in survival was analyzed with the Kruskal-Wallis test

[Differentiation of mesenchymal stem cells into cardiomyocytes induced by cardiomyocytes].

PubMed

Wang, Ting-Zhong; Ma, Ai-Qun; Xu, Zheng-Yun; Jiang, Wen-Hui; Du, Yuan

2005-06-01

To investigate the role of adult cardiomyocytes in the differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes. Rat MSCs were isolated by a Percoll's gradient solution and cultured in low-glucose Dulbecco' s modified Eagle' s medium (DMEM). After 2 passages, cell-surface antigen CD34, CD71 and CD90 for rat MSCs were determined by flow cytometry, and these MSCs were transfected with pEGFP-N3 by Lipofectamine2000. Then those MSCs labeled with GFP, were cultured in contacted, nocontacted and conditioned with adult rat myocardiocytes. Immunofluorescence staining against alpha-actin, desmin, and troponin-T were performed after 1 week. Immunofluorescence staining was positive against alpha-actin, desmin, and troponin-T on MSCs in contacted culture group. In contrast, no alpha-actin, desmin, and troponin-T expression on MSCs were observed in the noncontacted culture group and the conditioned culture group. Direct cell-to-cell contact between MSCs and adult cardiomyocytes may induce differentiation of MSCs into cardiomyocytes.

Comparison of soymilk, powdered milk, Hank's balanced salt solution and tap water on periodontal ligament cell survival.

PubMed

Moazami, Fariborz; Mirhadi, Hosein; Geramizadeh, Bita; Sahebi, Safoura

2012-04-01

The purpose of this study was to evaluate the ability of soymilk, powdered milk, and Hank's balanced salt solution (HBSS) to maintain human periodontal ligament (PDL) cell viability in vitro. PDL cells were obtained from extracted healthy third molars and cultured in Dulbecco's modified Eagles medium (DMEM). The cultures were exposed for 1, 2, 4, and 8 h to experimental solutions (tap water served as negative control and DMEM as positive control) at 37°C. The viable cells were then counted using the trypan blue exclusion technique. Data were analyzed by using one-way anova, post hoc Scheffe and two-way anova test. Statistical analysis showed that HBSS, powdered baby formula, and soymilk maintain cell viability equally well in different periods of times. Tap water cannot keep cells viable as well as other solutions. Soymilk and powdered baby formula can be recommended as suitable storage media for avulsed teeth for up to 8 h. © 2011 John Wiley & Sons A/S.

Effects of Ti and TiB2 Nanoparticulates on Room Temperature Mechanical Properties and In Vitro Degradation of Pure Mg

NASA Astrophysics Data System (ADS)

Meenashisundaram, Ganesh Kumar; Nai, Mui Hoon; Gupta, Manoj

Mg 1 vol.% Ti and Mg 1 vol.% TiB2 composites containing Ti (30-50 nm) and TiB2 ( 60 nm) nanoparticulates were successfully synthesized using disintegrated melt deposition technique followed by hot extrusion. In vitro degradation of synthesized pure magnesium and composites were assessed by immersion testing in Dulbecco's Modified Eagle's Medium (DMEM) + 10% Fetal Bovine Serum (FBS) solution for a maximum duration of 28 days. Determination of corrosion rates by weight loss technique reveals that after 28 days of immersion testing, Mg 1 vol.% Ti exhibited the best corrosion resistance followed by pure magnesium and finally by Mg 1 vol.% TiB2 composite. The room temperature mechanical properties of the synthesized composites were found to surpass those of pure magnesium. On tensile and compressive loading, substantial strengthening of pure magnesium was observed with 1 vol.% Ti addition whereas appreciable increase in tensile and compressive fracture strains of pure magnesium was observed with 1 vol.% TiB2 addition.

Persistent Effectivity of Gas Plasma-Treated, Long Time-Stored Liquid on Epithelial Cell Adhesion Capacity and Membrane Morphology

PubMed Central

Hoentsch, Maxi; Bussiahn, René; Rebl, Henrike; Bergemann, Claudia; Eggert, Martin; Frank, Marcus; von Woedtke, Thomas; Nebe, Barbara

2014-01-01

Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics. PMID:25170906

The Performance of the NAS HSPs in 1st Half of 1994

NASA Technical Reports Server (NTRS)

Bergeron, Robert J.; Walter, Howard (Technical Monitor)

1995-01-01

During the first six months of 1994, the NAS (National Airspace System) 16-CPU Y-MP C90 Von Neumann (VN) delivered an average throughput of 4.045 GFLOPS while the ACSF (Aeronautics Consolidated Supercomputer Facility) 8-CPU Y-MP C90 Eagle averaged 1.658 GFLOPS. The VN rate represents a machine efficiency of 26.3% whereas the Eagle rate corresponds to a machine efficiency of 21.6%. VN displayed a greater efficiency than Eagle primarily because the stronger workload demand for its CPU cycles allowed it to devote more time to user programs and less time to idle. An additional factor increasing VN efficiency was the ability of the UNICOS 8.0 Operating System to deliver a larger fraction of CPU time to user programs. Although measurements indicate increasing vector length for both workloads, insufficient vector lengths continue to hinder HSP (High Speed Processor) performance. To improve HSP performance, NAS should continue to encourage the HSP users to modify their codes to increase program vector length.

Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase.

PubMed

Pektaş, Mehtap; Kurt, Akif Hakan; Ün, İsmail; Tiftik, Rukiye Nalan; Büyükafşar, Kansu

2015-04-01

Effect of female sex hormones on the production/release of adipocyte-derived cytokines has been debatable. Furthermore, whether the cellular signaling triggered by these hormones involve Rho-kinase has not been investigated yet. Therefore, in this study, effects of 17β-estradiol and progesterone as well as the Rho-kinase inhibitor, Y-27632 on the level of adipokines such as resistin, adiponectin, leptin, TNF-α and IL-6 were investigated in 3T3-L1-derived adipocytes. Differentiation was induced in the post-confluent preadipocytes by the standard differentiation medium (Dulbecco's modified Eagle's medium with 10% fetal bovine serum together with the mixture of isobutylmethylxanthine, dexamethasone and insulin) in the presence of 17β-estradiol (10(-8)-10(-7)M), progesterone (10(-6)-10(-5)M), the Rho-kinase inhibitor, Y-27632 (10(-5)M) and their combination for 8days. Measurements of the adipokines were performed in the culturing medium by ELISA kits using specific monoclonal antibodies. 17β-estradiol elevated resistin but decreased adiponectin and IL-6 levels; however, it did not alter the concentration of leptin and TNF-α. Y-27632 pretreatment inhibited the rise of resistin and the fall of adiponectin by 17β-estradiol without any effects by its own. Progesterone did not change resistin, leptin and TNF-α level; however, it elevated adiponectin and decreased IL-6 production. Neither 17β-estradiol nor Y-27632 was able to antagonize the increase of adiponectin and the reduction of IL-6 levels by progesterone. While Y-27632 alone lowered IL-6 level, it increased leptin and TNF-α concentration without altering resistin and adiponectin. In conclusion, 17β-estradiol could modify adipokine production in 3T3-L1 adipocytes with the actions some of which involve Rho-kinase mediation. Copyright © 2015 Elsevier Ltd. All rights reserved.

Producing progeny from endangered birds of prey: Treatment of urine-contaminated semen and a novel intramagnal insemination approach

USGS Publications Warehouse

Blanco, J.M.; Gee, G.F.; Wildt, D.E.; Donoghue, A.M.

2002-01-01

Wild raptors brought into an ex situ environment often have poor semen quality that is further compromised by urine contamination. Generally, it is believed that in birds, artificial insemination into the cloaca or caudal vagina of females requires large doses of high-quality spermatozoa to maximize fertility. In an effort to define and overcome some of the challenges associated with reproduction in wild raptors, the objectives of this study were to 1) evaluate the frequency, impact, and remediation of urine contamination in fresh ejaculates for the purpose of maintaining sperm motility and viability in vitro, and 2) develop a deep insemination method that allows low numbers of washed sperm to be placed directly into the magnum to increase the probability of producing fertilized eggs. The species evaluated include golden eagle (Aquila chrysoetos), imperial eagle (A. adalberti), Bonelli's eagle (Hiernaetus fasciatus), and peregrine, falcon (Falco peregrinus). Semen samples were collected and pooled by species, and a minimum of 25 pooled ejaculates per species were evaluated for urine contamination, pH, sperm viability, and sperm motility; the samples were either unwashed or washed in neutral (pH 7.0) or alkaline (pH 8.0) modified Lake's diluent. Female golden eagles and peregrine falcons were inseminated via transjunctional, intramagnal insemination with washed spermatozoa from urine-contaminated samples. Urine contamination occurred in 36.8 +/- 12.8% (mean +/- SEM) golden eagle, 43.1 +/- 9.1% imperial eagle, 28.7 +/- 16.1% Bonelli's eagle, and 48.2 +/- 17.3% peregrine falcon ejaculates. The pH in urine-contaminated semen samples ranged from 6.48 +/- 0.3 to 6.86 +/- 0.2, and in noncontaminated samples it ranged from from 7.17 +/- 0.1 to 7.56 +/- 0.1. Sperm viability and motility were reduced (P < 0.05) in all species for unwashed vs. washed sperm after 30 min incubation at room temperature. Two peregrine falcon chicks and one golden eagle chick hatched after intramagnal insemination. This study demonstrates that urine contamination, a common and lethal acidifier in manually collected raptor ejaculates, can be circumvented by immediate, gentle seminal washing. Furthermore, these processed sperm, when deposited by transjunctional intramagnal insemination, can produce live young.

Interaction of carbohydrate modified boron nitride nanotubes with living cells.

PubMed

Emanet, Melis; Şen, Özlem; Çobandede, Zehra; Çulha, Mustafa

2015-10-01

Boron nitride nanotubes (BNNTs) are composed of boron and nitrogen atoms and they show significantly different properties from their carbon analogues (carbon nanotubes, CNTs). Due to their unique properties including low electrical conductivity, and imaging contrast and neutron capture properties; they can be used in biomedical applications. When their use in biological fields is considered, the route of their toxic effect should be clarified. Therefore, the study of interactions between BNNTs and living systems is important in envisaging biological applications at both cellular and sub-cellular levels to fully gain insights of their potential adverse effects. In this study, BNNTs were modified with lactose, glucose and starch and tested for their cytotoxicity. First, the interactions and the behavior of BNNTs with bovine serum albumin (BSA), Dulbecco's Modified Eagle's Medium (DMEM) and DMEM/Nutrient Mixture F-12Ham were investigated. Thereafter, their cellular uptake and the cyto- and genotoxicity on human dermal fibroblasts (HDFs) and adenocarcinoma human alveolar basal epithelial cells (A549) were evaluated. HDFs and A549 cells internalized the modified and unmodified BNNTs, and BNNTs were found to not cause significant viability change and DNA damage. A higher uptake rate of BNNTs by A549 cells compared to HDFs was observed. Moreover, a concentration-dependent cytotoxicity was observed on A549 cells while they were safer for HDFs in the same concentration range. Based on these findings, it can be concluded that BNNTs and their derivatives made with biomacromolecules might be good candidates for several applications in medicine and biomedical applications. Copyright © 2015 Elsevier B.V. All rights reserved.

Platelet lysate enhances synovial fluid multipotential stromal cells functions: Implications for therapeutic use.

PubMed

Altaie, Ala; Baboolal, Thomas G; Wall, Owen; Jones, Elena; McGonagle, Dennis

2018-03-01

Although intra-articular injection of platelet products is increasingly used for joint regenerative approaches, there are few data on their biological effects on joint-resident multipotential stromal cells (MSCs), which are directly exposed to the effects of these therapeutic strategies. Therefore, this study investigated the effect of platelet lysate (PL) on synovial fluid-derived MSCs (SF-MSCs), which in vivo have direct access to sites of cartilage injury. SF-MSCs were obtained during knee arthroscopic procedures (N = 7). Colony forming unit-fibroblast (CFU-F), flow-cytometric phenotyping, carboxyfluorescein succinimidyl ester-based immunomodulation for T-cell and trilineage differentiation assays were performed using PL and compared with standard conditions. PL-enhanced SF-MSC (PL-MSC) proliferation as CFU-F colonies was 1.4-fold larger, and growing cultures had shorter population-doubling times. PL-MSCs and fetal calf serum (FCS)-MSCs had the same immunophenotype and similar immunomodulation activities. In chondrogenic and osteogenic differentiation assays, PL-MSCs produced 10% more sulfated-glycosaminoglycan (sGAG) and 45% less Ca ++ compared with FCS-MSCs, respectively. Replacing chondrogenic medium transforming growth factor-β3 with 20% or 50% PL further increased sGAG production of PL-MSCs by 69% and 95%, respectively, compared with complete chondrogenic medium. Also, Dulbecco's Modified Eagle's Medium high glucose (HG-DMEM) plus 50% PL induced more chondrogenesis compared with HG-DMEM plus 10% FCS and was comparable to complete chondrogenic medium. This is the first study to assess SF-MSC responses to PL and provides biological support to the hypothesis that PL may be capable of modulating multiple functional aspects of joint resident MSCs with direct access to injured cartilage. Copyright © 2017 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

Induction of different activated phenotypes of mouse peritoneal macrophages grown in different tissue culture media.

PubMed

Kawakami, Tomoya; Koike, Atsushi; Amano, Fumio

2017-08-01

The role of activated macrophages in the host defense against pathogens or tumor cells has been investigated extensively. Many researchers have been using various culture media in in vitro experiments using macrophages. We previously reported that J774.1/JA-4 macrophage-like cells showed great differences in their activated macrophage phenotypes, such as production of reactive oxygen, nitric oxide (NO) or cytokines depending on the culture medium used, either F-12 (Ham's F-12 nutrient mixture) or Dulbecco modified Eagle's medium (DMEM). To examine whether a difference in the culture medium would influence the functions of primary macrophages, we used BALB/c mouse peritoneal macrophages in this study. Among the activated macrophage phenotypes, the expression of inducible NO synthase in LPS- and/or IFN-γ-treated peritoneal macrophages showed the most remarkable differences between F-12 and DMEM; i.e., NO production by LPS- and/or IFN-γ-treated cells was far lower in DMEM than in F-12. Similar results were obtained with C57BL mouse peritoneal macrophages. Besides, dilution of F-12 medium with saline resulted in a slight decrease in NO production, whereas that of DMEM with saline resulted in a significant increase, suggesting the possibility that DMEM contained some inhibitory factor(s) for NO production. However, such a difference in NO production was not observed when macrophage-like cell lines were examined. These results suggest that phenotypes of primary macrophages could be changed significantly with respect to host inflammatory responses by the surrounding environment including nutritional factors and that these altered macrophage phenotypes might influence the biological host defense.

Modulation of chondrogenic differentiation of human mesenchymal stem cells in jellyfish collagen scaffolds by cell density and culture medium.

PubMed

Pustlauk, W; Paul, B; Brueggemeier, S; Gelinsky, M; Bernhardt, A

2017-06-01

Studies on tissue-engineering approaches for the regeneration of traumatized cartilage focus increasingly on multipotent human mesenchymal stem cells (hMSCs) as an alternative to autologous chondrocytes. The present study applied porous scaffolds made of collagen from the jellyfish Rhopilema esculentum for the in vitro chondrogenic differentiation of hMSCs. Culture conditions in those scaffolds differ from conditions in high-density pellet cultures, making a re-examination of these data necessary. We systematically investigated the influence of seeding density, basic culture media [Dulbecco's modified Eagle's medium (DMEM), α-minimum essential medium (α-MEM)] with varying glucose content and supplementation with fetal calf serum (FCS) or bovine serum albumin (BSA) on the chondrogenic differentiation of hMSCs. Gene expression analyses of selected markers for chondrogenic differentiation and hypertrophic development were conducted. Furthermore, the production of cartilage extracellular matrix (ECM) was analysed by quantification of sulphated glycosaminoglycan and collagen type II contents. The strongest upregulation of chondrogenic markers, along with the highest ECM deposition was observed in scaffolds seeded with 2.4 × 10 6 cells/cm 3 after cultivation in high-glucose DMEM and 0.125% BSA. Lower seeding densities compared to high-density pellet cultures were sufficient to induce in vitro chondrogenic differentiation of hMSCs in collagen scaffolds, which reduces the amount of cells required for the seeding of scaffolds and thus the monolayer expansion period. Furthermore, examination of the impact of FCS and α-MEM on chondrogenic MSC differentiation is an important prerequisite for the development of an osteochondral medium for simultaneous osteogenic and chondrogenic differentiation in biphasic scaffolds for osteochondral tissue regeneration. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

77 FR 13592 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-07

...; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land Resources, LLC; Notice... 24, 2012, AER NY-Gen, LLC (transferor), Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources.... Cherry, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources...

75 FR 27332 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-14

... 9690-106] AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek... Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC (transferees) filed an.... Paul Ho, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources...

77 FR 42714 - Eagle Creek Hydropower, LLC, Eagle Creek Land Resources, LLC, Eagle Creek Water Resources, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... Hydropower, LLC, Eagle Creek Land Resources, LLC, Eagle Creek Water Resources, LLC; Notice of Application...: Eagle Creek Hydropower, LLC; Eagle Creek Land Resources, LLC; and Eagle Creek Water Resources, LLC. e... Contact: Robert Gates, Senior Vice President-- Operations, Eagle Creek Hydropower, LLC, Eagle Creek Water...

FTIR characterization of animal lung cells: normal and precancerous modified e10 cell line

NASA Astrophysics Data System (ADS)

Zezell, D. M.; Pereira, T. M.; Mennecier, G.; Bachmann, L.; Govone, A. B.; Dagli, M. L. Z.

2012-06-01

The chemical carcinogens from tobacco are related to over 90% of lung cancers around the world. The risk of death of this kind of cancer is high because the diagnosis usually is made only in advanced stages. Therefore, it is necessary to develop new diagnostic methods for detecting the lung cancer in earlier stages. The Fourier Transform Infrared Spectroscopy (FTIR) can offer high sensibility and accuracy to detect the minimal chemical changes into the biological sample. The aim of this study is to evaluate the differences on infrared spectra between normal lung cells and precancerous lung cells transformed by NNK. Non-cancerous lung cell line e10 (ATCC) and NNK-transformed e10 cell lines were maintained in complete culture medium (1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 [DMEM/Ham's F12], supplemented with 100 ng/ml cholera enterotoxin, 10 lg/ml insulin, 0.5 lg/ml. hydrocortisol, 20 ng/ml epidermal growth factor, and 5% horse serum. The cultures were maintained in alcohol 70%. The infrared spectra were acquired on ATR-FTIR Nicolet 6700 spectrophotometer at 4 cm-1 resolution, 30 scans, in the 1800-900 cm-1 spectral range. Each sample had 3 spectra recorded, 30 infrared spectra were obtained from each cell line. The second derivate of spectra indicates that there are displacement in 1646 cm-1 (amine I) and 1255 cm-1(DNA), allowing the possibility to differentiate the two king of cells, with accuracy of 89,9%. These preliminary results indicate that ATR-FTIR is useful to differentiate normal e10 lung cells from precancerous e10 transformed by NNK.

Airmobile Shelter Analysis. Volume 2

DTIC Science & Technology

1994-02-01

effort are: 10 Shelter functions: billets, command and control, administration, maintenance shops, warehousing, medical, kitchens , dining halls...military inventory. The Harvest Eagle family of shelters contains three pole-supported tents - the GP Medium and GP Large tents, and the M-1948 kitchen I...Supported i...brids -PassiveT -Load-Bearing Active Panel Walls Figure 2. Hierarchy of Shelter Categories. vIV]. The M-1948 kitchen has been replaced by

Radiolabeling of infective third-stage larvae of Strongyloides stercoralis by feeding ( sup 75 Se)selenomethionine-labeled Escherichia coli to first- and second-stage larvae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aikens, L.M.; Schad, G.A.

1989-10-01

A technique is described for radiolabeling Strongyloides stercoralis larvae with ({sup 75}Se)selenomethionine. Cultures of an auxotrophic methionine-dependent stain of Escherichia coli were grown in a medium containing Dulbecco's modified Eagle's medium supplemented with 5% nutrient broth, amino acids, and ({sup 75}Se)selenomethionine. When the {sup 75}Se-labeled bacterial populations were in the stationary phase of growth, cultures were harvested and the bacteria dispersed on agar plates to serve as food for S. stercoralis larvae. Use of nondividing bacteria is important for successful labeling because the isotope is not diluted by cell division and death of larvae attributable to overgrowth by bacteria ismore » prevented. First-stage S. stercoralis larvae were recovered from feces of infected dogs and reared in humid air at 30 C on agar plates seeded with bacteria. After 7 days, infective third-stage larvae were harvested. The mean specific activity of 6 different batches of larvae ranged from 75 to 330 counts per min/larva with 91.8 +/- 9.5% of the population labeled sufficiently to produce an autoradiographic focus during a practicable, 6-wk period of exposure. Labeled infective larvae penetrated the skin of 10-day-old puppies and migrated to the small intestine, where the developed to adulthood.« less

Defined xenogeneic-free and hypoxic environment provides superior conditions for long-term expansion of human adipose-derived stem cells.

PubMed

Yang, Sufang; Pilgaard, Linda; Chase, Lucas G; Boucher, Shayne; Vemuri, Mohan C; Fink, Trine; Zachar, Vladimir

2012-08-01

Development and implementation of therapeutic protocols based on stem cells or tissue-engineered products relies on methods that enable the production of substantial numbers of cells while complying with stringent quality and safety demands. In the current study, we aimed to assess the benefits of maintaining cultures of adipose-derived stem cells (ASCs) in a defined culture system devoid of xenogeneic components (xeno-free) and hypoxia over a 49-day growth period. Our data provide evidence that conditions involving StemPro mesenchymal stem cells serum-free medium (SFM) Xeno-Free and hypoxia (5% oxygen concentration) in the culture atmosphere provide a superior proliferation rate compared to a standard growth environment comprised of alpha-modified Eagle medium (A-MEM) supplemented with fetal calf serum (FCS) and ambient air (20% oxygen concentration) or that of A-MEM supplemented with FCS and hypoxia. Furthermore, a flow cytometric analysis and in vitro differentiation assays confirmed the immunophenotype stability and maintained multipotency of ASCs when expanded under xeno-free conditions and hypoxia. In conclusion, our data demonstrate that growth conditions utilizing a xeno-free and hypoxic environment not only provide an improved environment for the expansion of ASCs, but also set the stage as a culture system with the potential broad spectrum utility for regenerative medicine and tissue engineering applications.

Magnesium degradation observed in situ under flow by synchrotron radiation based microtomography

NASA Astrophysics Data System (ADS)

Feyerabend, Frank; Dose, Thomas; Xu, Yuling; Beckmann, Felix; Stekker, Michael; Willumeit-Römer, Regine; Schreyer, Andreas; Wilde, Fabian; Hammel, Jörg U.

2016-10-01

The use of degradable magnesium based implants is becoming clinically relevant, e.g. for the use as bone screws. Still there is a lack of analyzing techniques to characterize the in vitro degradation behavior of implant prototypes. The aim of this study was to design an in situ environment to continuously monitor the degradation processes under physiological conditions by time-lapse SRμCT. The use of physiological conditions was chosen to get a better approach to the in vivo situation, as it could be shown by many studies, that these conditions change on the one hand the degradation rate and on the other hand also the formed degradation products. The resulting in situ environment contains a closed bioreactor system to control and monitor the relevant parameters (37°C, 5 % O2, 20 % CO2) and to grant sterility of the setup. A flow cell was designed and manufactured from polyether etherketone (PEEK), which was chosen because of the good mechanical properties, high thermal and chemical resistance and radiographic translucency. Sterilization of the system including the sample was reached by a transient flush with 70 % ethanol and subsequent replacement by physiological medium (Modified Eagle Medium alpha). As proof of principle it could be shown that the system remained sterile during a beamtime of several days and that the continuous SRμCT imaging was feasible.

Influence of artificial biological fluid composition on the biocorrosion of potential orthopedic Mg-Ca, AZ31, AZ91 alloys.

PubMed

Gu, X N; Zheng, Y F; Chen, L J

2009-12-01

The electrochemical behavior of potential orthopedic Mg-Ca, AZ31 and AZ91 alloys was studied in Hank's solution, Dulbecco's Modified Eagle's Medium (DMEM) and serum-containing medium (DMEM adding 10% fetal bovine serum (DMEM+FBS)) over a 7 day immersion period. The biocorrosion of the above three alloys for various immersion time intervals was investigated by linear polarization and electrochemical impedance spectroscopy (EIS). After 7 day immersion, potentiodynamic polarization tests were carried out and the surface morphologies of experimental samples were examined by scanning electron microscopy (SEM) observation complemented by energy-disperse spectrometer (EDS) analysis. It was shown that the corrosion of magnesium alloys was influenced by the composition of the solution. The results indicated that chloride ion could reduce the corrosion resistance and the hydrocarbonate ions could induce rapid surface passivation. The adsorbed amino acid on the experimental magnesium alloys' surface increased their polarization resistance and reduced current densities. The influence of the serum protein on corrosion was found to be associated with the magnesium alloy compositions. A Mg-Ca alloy exhibited an increased corrosion rate in the presence of serum protein. An AZ31 alloy showed an increased corrosion rate in DMEM+FBS in the initial 3 day immersion and the corrosion rate decreased thereafter. An AZ91 alloy, with high Al content, showed a reduced corrosion rate with the addition of FBS into DMEM.

Study of the interaction of inorganic and organic compounds of cell culture medium with a Ti surface.

PubMed

Burgos-Asperilla, L; García-Alonso, M C; Escudero, M L; Alonso, C

2010-02-01

The interaction between Ti and each component of Dulbecco's modified Eagle's medium was studied in depth using different techniques, such as the measurement of the corrosion potential, electrochemical impedance spectroscopy and polarization curves. The characterization of metal surfaces was carried out by scanning electron microscopy and X-ray photoelectron spectroscopy (XPS). The adsorption process of each component was studied using the quartz crystal balance (QCM). The QCM and XPS results reveal that the adsorption kinetics for phosphate and calcium ions is slow. However, the bovine serum albumin (BSA) totally covers the Ti surface rapidly. Because the passive film (titanium oxide) has acidic hydroxyl groups, the calcium ions would have a bridging effect on the electrostatic adsorption of phosphate ions as well as that of BSA. The polarization curves reveal that the adsorbed glucose permits the ionic diffusion of the oxygen to the electrode, while the BSA and fetal bovine serum (FBS) adsorbed after 7 days of immersion act as a diffusive barrier. The impedance measurement and data fitting to the electrical equivalent circuit model show that the resistance of the proteins/TiO(2) interface, for Ti immersed in FBS, is higher than those obtained for BSA, due to the proteins present in the solution as well as the fact that the adsorbed proteins on the surface are greater.

Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts

PubMed Central

Yamada, Takuya; Onuma, Kenji; Kuzuno, Jun; Ujihira, Masanobu; Kurokawa, Ryosuke; Sakai, Rina; Takaso, Masashi

2014-01-01

Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco's Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting. PMID:25506061

L-Cysteine-induced up-regulation of the low-density lipoprotein receptor is mediated via a transforming growth factor-alpha signalling pathway.

PubMed

Tanaka, Yuma; Shimada, Masaya; Nagaoka, Satoshi

2014-02-14

Sulphur-containing amino acids regulate plasma cholesterol levels in animals and humans. However, their mechanism of action remains unclear. Low-density lipoprotein receptor (LDLR) plays an important role in cholesterol metabolism. We therefore investigated the effects of sulphur-containing amino acids on the expression of LDLR in hepatocytes. HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium with or without sulphur-containing amino acids and cysteine-containing compounds. We found that L-cysteine increased LDLR mRNA and enhanced LDLR gene promoter activity through the extracellular-signal-related kinase and p38 mitogen-activated protein kinase signalling pathways in HepG2 cells. Moreover, we observed that L-cysteine stimulated the release of transforming growth factor-alpha (TGF-α) and that TGF-α increased the LDLR mRNA levels. This study provides a report of the L-cysteine mediated up-regulation of the LDLR expression via TGF-α signalling pathway. Our findings provide insights into cholesterol homeostasis and amino acid signalling. Copyright © 2014 Elsevier Inc. All rights reserved.

Influence of culture media on the physical and chemical properties of Ag-TiCN coatings

NASA Astrophysics Data System (ADS)

Carvalho, I.; Escobar Galindo, R.; Henriques, M.; Palacio, C.; Carvalho, S.

2014-08-01

The aim of this study was to verify the possible physical and chemical changes that may occur on the surface of Ag-TiCN coatings after exposure to the culture media used in microbiological and cytotoxic assays, respectively tryptic soy broth (TSB) and Dulbecco's modified eagle's medium (DMEM). After sample immersion for 24 h in the media, analyses were performed by glow discharge optical emission spectroscopy discharge radiation (GDOES), Rutherford backscattering spectroscopy (RBS) and x-ray photoelectron spectroscopy (XPS). The results of GDOES profile, RBS and XPS spectra, of samples immersed in TSB, demonstrated the formation of a thin layer of carbon, oxygen and nitrogen that could be due to the presence of proteins in TSB. After 24 h of immersion in DMEM, the results showed the formation of a thin layer of calcium phosphates on the surface, since the coatings displayed a highly oxidized surface in which calcium and phosphorus were detected. All these results suggested that the formation of a layer on the coating surface prevented the release of silver ions in concentrations that allow antibacterial activity.

Retinal pigment epithelium culture;a potential source of retinal stem cells.

PubMed

Akrami, Hassan; Soheili, Zahra-Soheila; Khalooghi, Keynoush; Ahmadieh, Hamid; Rezaie-Kanavi, Mojgan; Samiei, Shahram; Davari, Malihe; Ghaderi, Shima; Sanie-Jahromi, Fatemeh

2009-07-01

To establish human retinal pigment epithelial (RPE) cell culture as a source for cell replacement therapy in ocular diseases. Human cadaver globes were used to isolate RPE cells. Each globe was cut into several pieces of a few millimeters in size. After removing the sclera and choroid, remaining tissues were washed in phosphate buffer saline and RPE cells were isolated using dispase enzyme solution and cultured in Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 supplemented with 10% fetal calf serum. Primary cultures of RPE cells were established and spheroid colonies related to progenitor/stem cells developed in a number of cultures. The colonies included purely pigmented or mixed pigmented and non-pigmented cells. After multiple cellular passages, several types of photoreceptors and neural-like cells were detected morphologically. Cellular plasticity in RPE cell cultures revealed promising results in terms of generation of stem/progenitor cells from human RPE cells. Whether the spheroids and neural-like retinal cells were directly derived from retinal stem cells or offspring of trans-differentiating or de-differentiating RPE cells remains to be answered.

Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media.

PubMed

Kleinsimlinghaus, Karolina; Marx, Romy; Serdar, Meray; Bendix, Ivo; Dietzel, Irmgard D

2013-01-01

The aim of the present study has been to obtain high yields of oligodendrocyte precursor cells (OPCs) in culture. This is a first step in facilitation of myelin repair. We show that, in addition to factors, known to promote proliferation, such as basic fibroblast growth factor (FGF-2) and platelet derived growth factor (PDGF) the choice of the basal medium exerts a significant influence on the yield of OPCs in cultures from newborn rats. During a culture period of up to 9 days we observed larger numbers of surviving cells in Dulbecco's Modified Eagle Medium (DMEM), and Roswell Park Memorial Institute Medium (RPMI) compared with Neurobasal Medium (NB). A larger number of A2B5-positive OPCs was found after 6 days in RPMI based media compared with NB. The percentage of bromodeoxyuridine (BrdU)-positive cells was largest in cultures maintained in DMEM and RPMI. The percentage of caspase-3 positive cells was largest in NB, suggesting that this medium inhibits OPC proliferation and favors apoptosis. A difference between NB and DMEM as well as RPMI is the reduced Na(+)-content. The addition of equiosmolar supplements of mannitol or NaCl to NB medium rescued the BrdU-incorporation rate. This suggested that the osmolarity influences the proliferation of OPCs. Plating density as well as residual microglia influence OPC survival, BrdU incorporation, and caspase-3 expression. We found, that high density cultures secrete factors that inhibit BrdU incorporation whereas the presence of additional microglia induces an increase in caspase-3 positive cells, indicative of enhanced apoptosis. An enhanced number of microglia could thus also explain the stronger inhibition of OPC differentiation observed in high density cultures in response to treatment with the cytokines TNF-α and IFN-γ. We conclude that a maximal yield of OPCs is obtained in a medium of an osmolarity higher than 280 mOsm plated at a relatively low density in the presence of as little microglia as technically achievable.

Analytical nanosphere sensors using quantum dot-enzyme conjugates for urea and creatinine.

PubMed

Ruedas-Rama, Maria J; Hall, Elizabeth A H

2010-11-01

An enzyme-linked analytical nanosphere sensor (ANSor) is described, responding to enzyme-substrate turnover in the vicinity of a quantum dot (QD) due to coimmobilized enzyme and pH sensitive ligand. QD capping by mercapto-alkanoic acids were rejected as a pH sensitive ligand, but with the use of a layer-by-layer assembly on mercaptopropionic capped QDs and an intermediate poly(allylamine hydrochloride) layer, anthraquinone sulfonate (calcium red, CaR) was introduced to modify the pKa in the immobilized system > 8. QD-CaR absorption shows spectral overlap with QD530 emission at all pHs and gives a complex pH dependent fluorescence resonance energy transfer (FRET) efficiency, due to excited state proton transfer (λ(ex) = 540 nm; λ(em) = 585 nm). In contrast QD615-CaR with spectral overlap between the QD and CaR gave a strong and reproducible pH response. QD-urease and QD-creatinine deiminase conjugates could be linked with pH changes produced by enzyme degradation of urea and creatinine, respectively. Close coupling between the pH sensitive QD and enzyme conjugate maximized signal compared with solution based assays: QD-urease and QD-CD bioconjugates were tested in model biological media (Dulbecco's modified Eagle's Medium and fetal calf serum) and in urine, showing a response in 3-4 min.

Surface Wave Dispersion Measurements and Tomography From Ambient Seismic Noise in China

DTIC Science & Technology

2007-12-20

Recovering the Green’s function from field - field correlations in an open scattering medium (L), J. Acoust. Soc. Amer. 113 (6), 2973- 2976, 2003. Eagle, D...The basic approach can be traced back much earlier studies of random fields in seismology (Aki, 1957; Toksoz, 1964; Claerbout, 1968), in...Seismic Network (CNDSN), Center of China Digital Seismic Network (CCDSN) stations, and China Seismic Network ( CSN ). We refer here as China National

Response of potted northern red oak and hay-scented fern to additions of calcium, magnesium, potassium, and phosphorus

Treesearch

David H. Hart; William E. Sharpe

1997-01-01

The objective of this study was to determine if addition of Ca, Mg, K, and P to an extremely acidic forest soil would increase early northern red oak (Quercus rubra L.) seedling growth. The O through B2 horizon of a Dekalb soil from Pennsylvania's Bald Eagle State Forest was placed in plastic cores and utilized as a growth medium for northern...

Survey for hemoparasites in imperial eagles (Aquila heliaca), steppe eagles (Aquila nipalensis), and white-tailed sea eagles (Haliaeetus albicilla) from Kazakhstan.

PubMed

Leppert, Lynda L; Layman, Seth; Bragin, Evgeny A; Katzner, Todd

2004-04-01

Prevalence of hemoparasites has been investigated in many avian species throughout Europe and North America. Basic hematologic surveys are the first step toward evaluating whether host-parasite prevalences observed in North America and Europe occur elsewhere in the world. We collected blood smears from 94 nestling imperial eagles (Aquila heliaca), five nestling steppe eagles (Aquila nipalensis), and 14 nestling white-tailed sea eagles (Haliaeetus albicilla) at Naurzum Zapovednik (Naurzum National Nature Reserve) in Kazakhstan during the summers of 1999 and 2000. In 1999, six of 29 imperial eagles were infected with Lencocytozoon toddi. Five of 65 imperial eagles and one of 14 white-tailed sea eagle were infected with L. toddi in 2000. Furthermore, in 2000, one of 65 imperial eagles was infected with Haemoproteus sp. We found no parasites in steppe eagles in either year, and no bird had multiple-species infections. These data are important because few hematologic studies of these eagle species have been conducted.

Optimized method of dispersion of titanium dioxide nanoparticles for evaluation of safety aspects in cosmetics

NASA Astrophysics Data System (ADS)

Carvalho, Karina Penedo; Martins, Nathalia Balthazar; Ribeiro, Ana Rosa Lopes Pereira; Lopes, Taliria Silva; de Sena, Rodrigo Caciano; Sommer, Pascal; Granjeiro, José Mauro

2016-08-01

Nanoparticles agglomerate when in contact with biological solutions, depending on the solutions' nature. The agglomeration state will directly influence cellular response, since free nanoparticles are prone to interact with cells and get absorbed into them. In sunscreens, titanium dioxide nanoparticles (TiO2-NPs) form mainly aggregates between 30 and 150 nm. Until now, no toxicological study with skin cells has reached this range of size distribution. Therefore, in order to reliably evaluate their safety, it is essential to prepare suspensions with reproducibility, irrespective of the biological solution used, representing the above particle size distribution range of NPs (30-150 nm) found on sunscreens. Thus, the aim of this study was to develop a unique protocol of TiO2 dispersion, combining these features after dilution in different skin cell culture media, for in vitro tests. This new protocol was based on physicochemical characteristics of TiO2, which led to the choice of the optimal pH condition for ultrasonication. The next step consisted of stabilization of protein capping with acidified bovine serum albumin, followed by an adjustment of pH to 7.0. At each step, the solutions were analyzed by dynamic light scattering and transmission electron microscopy. The final concentration of NPs was determined by inductively coupled plasma-optical emission spectroscopy. Finally, when diluted in dulbecco's modified eagle medium, melanocytes growth medium, or keratinocytes growth medium, TiO2-NPs displayed a highly reproducible size distribution, within the desired size range and without significant differences among the media. Together, these results demonstrate the consistency achieved by this new methodology and its suitability for in vitro tests involving skin cell cultures.

Study of the effectiveness of propolis extract as a storage medium for avulsed teeth.

PubMed

Casaroto, Ana Regina; Hidalgo, Mirian Marubayashi; Sell, Ana Maria; Franco, Selma Lucy; Cuman, Roberto Kenji Nakamura; Moreschi, Eduardo; Victorino, Fausto Rodrigo; Steffens, Vânia Antunes; Bersani-Amado, Ciomar Aparecida

2010-08-01

The purpose of the present study was to evaluate the efficacy of propolis extract in maintaining the viability of human periodontal ligament (PDL) cells, and to radiographically analyze tooth replantation and the adjacent periodontium in dogs after storage in this extract. Human PDL cells were incubated with the experimental media propolis, milk, saliva, Hank's balanced salt solution (HBSS), and Dulbecco's modified Eagles medium (DMEM, positive controls), and distilled water (negative control). Cell viability was determined 0, 1, 3, 6, 12, and 24 h later by colorimetric MTT assay. Thirty incisors from dogs were divided into two storage time blocks (1 and 3 h) and were maintained in the experimental media. HBSS served as a positive control, and dry teeth (on gauze) as a negative control. The replanted teeth were radiographed once per month for 6 months. The radiographic images were standardized by the shortening/lengthening factor, and were both qualitatively and quantitatively analyzed. The in vitro results showed that the efficacy of propolis in maintaining functional viability of PDL cells was similar to that of milk. Propolis and milk were significantly better than controls from the 6-h time period. The in vivo results showed that teeth maintained in propolis medium exhibited replacement resorption with significant reduction in tooth length, similar to teeth maintained in saliva and dried teeth. This resorption was less intense with the 3-h storage time than the 1-h storage time. Conditions close to normal were found in teeth maintained in milk, similar to the HBSS control. Therefore, although propolis was effective in maintaining the viability of human PDL cells, resorption of the tooth replantation in dogs occurred under these experimental conditions.

Short communication: Arginase inhibition reduces the synthesis of casein in bovine mammary epithelial cells.

PubMed

Wang, M Z; Ding, L Y; Wang, C; Chen, L M; Loor, J J; Wang, H R

2017-05-01

The main purpose of this work was to determine the effect of arginase inhibition via N ω -hydroxy-nor-l-arginine (nor-NOHA) on casein synthesis in bovine mammary epithelial cells (BMEC). Passage 2 BMEC isolated from dairy cows were seeded to 6-well plates and randomly divided into 4 treatments: (1) control [Dulbecco's modified Eagle medium:Nutrient Mixture F-12 medium (DMEM/F12)]; (2) nor-NOHA (DMEM/F12 + 1 mmol/L nor-NOHA); (3) nor-NOHA + arginine (DMEM/F12 + 1 mmol/L nor-NOHA + 3.2 mmol/L Arg); and (4) nor-NOHA + ornithine (DMEM/F12+ 1 mmol/L nor-NOHA + 1 mmol/L Orn). Then, we determined the activity of enzymes related to Arg metabolism and casein synthesis in BMEC and the proliferation of cells. The addition of nor-NOHA reduced the activity of arginase and ornithine decarboxylase but had no effect on the activity of nitric oxide synthase, and these responses were the same at the gene expression level. In addition, supplementation of nor-NOHA in BMEC reduced cellular proliferation and casein synthesis. Addition of Arg to nor-NOHA resulted in cellular proliferation and casein synthesis similar to that of nor-NOHA alone. In contrast, addition of Orn to the medium with nor-NOHA increased the synthesis of casein and cellular proliferation compared with Nor-NOHA. In conclusion, suppression of the Arg-arginase-Orn pathway reduced casein synthesis and cellular proliferation, which indicated that this pathway is an important regulator of the synthesis of casein in BMEC. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

78 FR 65238 - Proposed Establishment of Class E Airspace; Eagle, AK

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

...-0777; Airspace Docket No. 12-AAL-16] Proposed Establishment of Class E Airspace; Eagle, AK AGENCY... action proposes to establish Class E airspace at Eagle Airport, Eagle, AK. Controlled airspace is... management of aircraft operations at Eagle Airport, Eagle, AK. DATES: Comments must be received on or before...

CHOLESTEROL REQUIREMENT OF PRIMARY DIPLOID HUMAN FIBROBLASTS

PubMed Central

Holmes, Richard; Helms, Judy; Mercer, Gretchen

1969-01-01

Primary cultures of fibroblast-like cells were obtained from skin and articular cartilage of human donors in the age bracket of 1 to 15 years. For growth these cultures required 1 mg/liter of cholesterol added to Medium A2 plus acetyl choline, Na pyruvate, and D-galactosamine HCl (APG) containing 10% lipoprotein-free human serum. Established cell lines did not require cholesterol for growth. Eagle's medium could be used in place of Medium A2 plus APG with the same results. Desmosterol could replace cholesterol but lansterol or 7 dehydrocholesterol could not. Other cholesterol precursors were tested and found to be inactive. With the proviso that cholesterol precursors entered the cell and had to be converted to cholesterol to function, it was concluded that the particular primaries studied lacked a functional enzyme system to reduce the double bond at carbon 7. PMID:5786984

Seropositivity and Risk Factors Associated with Toxoplasma gondii Infection in Wild Birds from Spain

PubMed Central

Cabezón, Oscar; García-Bocanegra, Ignacio; Molina-López, Rafael; Marco, Ignasi; Blanco, Juan M.; Höfle, Ursula; Margalida, Antoni; Bach-Raich, Esther; Darwich, Laila; Echeverría, Israel; Obón, Elena; Hernández, Mauro; Lavín, Santiago; Dubey, Jitender P.; Almería, Sonia

2011-01-01

Toxoplasma gondii is a zoonotic intracellular protozoan parasite of worldwide distribution that infects many species of warm-blooded animals, including birds. To date, there is scant information about the seropositivity of T. gondii and the risk factors associated with T. gondii infection in wild bird populations. In the present study, T. gondii infection was evaluated on sera obtained from 1079 wild birds belonging to 56 species (including Falconiformes (n = 610), Strigiformes (n = 260), Ciconiiformes (n = 156), Gruiformes (n = 21), and other orders (n = 32), from different areas of Spain. Antibodies to T. gondii (modified agglutination test, MAT titer ≥1∶25) were found in 282 (26.1%, IC95%:23.5–28.7) of the 1079 birds. This study constitute the first extensive survey in wild birds species in Spain and reports for the first time T. gondii antibodies in the griffon vulture (Gyps fulvus), short-toed snake-eagle (Circaetus gallicus), Bonelli's eagle (Aquila fasciata), golden eagle (Aquila chrysaetos), bearded vulture (Gypaetus barbatus), osprey (Pandion haliaetus), Montagu's harrier (Circus pygargus), Western marsh-harrier (Circus aeruginosus), peregrine falcon (Falco peregrinus), long-eared owl (Asio otus), common scops owl (Otus scops), Eurasian spoonbill (Platalea leucorodia), white stork (Ciconia ciconia), grey heron (Ardea cinerea), common moorhen (Gallinula chloropus); in the International Union for Conservation of Nature (IUCN) “vulnerable” Spanish imperial eagle (Aquila adalberti), lesser kestrel (Falco naumanni) and great bustard (Otis tarda); and in the IUCN “near threatened” red kite (Milvus milvus). The highest seropositivity by species was observed in the Eurasian eagle owl (Bubo bubo) (68.1%, 98 of 144). The main risk factors associated with T. gondii seropositivity in wild birds were age and diet, with the highest exposure in older animals and in carnivorous wild birds. The results showed that T. gondii infection is widespread and can be at a high level in many wild birds in Spain, most likely related to their feeding behaviour. PMID:22216311

l-Glutamine Attenuates Apoptosis Induced by Endoplasmic Reticulum Stress by Activating the IRE1α-XBP1 Axis in IPEC-J2: A Novel Mechanism of l-Glutamine in Promoting Intestinal Health

PubMed Central

Chen, Jiashun; Liu, Shaojuan; Yao, Kang; Yin, Yulong

2017-01-01

Intestinal absorption and barrier malfunctions are associated with endoplasmic reticulum stress (ERS) in the intestine. We induced ERS by exposing the intestinal porcine epithelial cell line J2 (IPEC-J2) to tunicamycin (TUNI) to explore the potential of l-glutamine to reduce ERS-induced apoptosis. Our experiments demonstrated that exposing cells to TUNI results in spontaneous ERS and encourages the upregulation of glucose-regulated protein 78 (GRP78). Prolonged TUNI-induced ERS was found to increase apoptosis mediated by C/enhancer binding protein homologous protein (CHOP), accompanied by GRP78 downregulation. Treatment with l-glutamine was found to promote cell proliferation within the growth medium but to have little effect in basic Dulbecco’s modified Eagle medium. Finally, in the milieu of TUNI-induced ERS, l-glutamine was found to maintain a high level of GRP78, alleviate CHOP-mediated apoptosis and activate the inositol requiring enzyme 1α (IRE1α)-X-box binding protein 1 (XBP1) axis. A specific inhibitor of the IRE1α-XBP1 axis reversed the protective effect of l-glutamine by blocking the expression of IRE1α/XBP1s. We propose that the functional effect of l-glutamine on intestinal health may be partly due to its modulation of ERS and CHOP-mediated apoptosis. PMID:29206200

Effects of keratinocyte growth factor on skin epithelial differentiation of human amnion epithelial cells.

PubMed

Fatimah, Simat Siti; Tan, Geok Chin; Chua, Kienhui; Tan, Ay Eeng; Nur Azurah, Abdul Ghani; Hayati, Abdul Rahman

2013-08-01

The aim of the present study was to determine the effects of KGF on the differentiation of cultured human amnion epithelial cells (HAECs) towards skin keratinocyte. HAECs at passage 1 were cultured in medium HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of KGF (0, 5, 10, 20, 30 and 50 ng/ml KGF). Dose-response of KGF on HAECs was determined by morphological assessment; growth kinetic evaluation; immunocytochemical analysis; stemness and epithelial gene expression quantification with two step real time RT-PCR. KGF promotes the proliferation of HAECs with maximal effect observed at 10 ng/ml KGF. However, KGF decreased the stemness genes expression: Oct-3/4, Sox-2, Nanog3, Rex-1, FGF-4, FZD-9 and BST-1. KGF also down-regulates epithelial genes expression: CK3, CK18, CK19, Integrin-β1, p63 and involucrin in cultured HAECs. No significant difference on the gene expression was detected for each Nestin, ABCG-2, CK1 and CK14 in KGF-treated HAECs. Immunocytochemical analysis for both control and KGF-treated HAECs demonstrated positive staining against CK14 and CK18 but negative staining against involucrin. The results suggested that KGF stimulates an early differentiation of HAECs towards epidermal cells. Differentiation of KGF-treated HAECs to corneal lineage is unfavourable. Therefore, further studies are needed to elucidate the roles of KGF in the differentiation of HAECs towards skin keratinocytes. Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.

Survival of spumavirus, a primate retrovirus, in laboratory media and water.

PubMed

Lotlikar, Madhavi S; Lipson, Steven M

2002-06-04

The persistence of a previously characterized spumavirus strain (strain SV-522) was investigated utilizing various laboratory media and waters, including Eagle's minimal essential medium (EMEM) plus 0% fetal bovine serum (EMEM-0%), EMEM-2%, EMEM-10%, Chlamydia transport medium (CTM), phosphate-buffered saline, distilled, estuarine, and marine water, human serum, and the germicides, ethyl alcohol (70%) and sodium hypochlorite (10%). Experiments were performed at 4 degrees C and/or 23 degrees C. Infectivity endpoints were determined in stock aliquots upon initiation of testing and then after 3, 5, 7, and 10 days. The virus was reisolated from all diluents after 5 days at 23 degrees C and in EMEM-10% after 7 days. The virus was detected in CTM, EMEM-2%, EMEM-10%, and estuarine and marine waters after 7 days at 4 degrees C. Differences in the persistence of the virus may be ascribed to temperature and organic load. Water ionic strengths (e.g., estuarine vs. marine water) had no effect on modifying persistence of viral particles. Infectivity of spumavirus was undetectable after 30 s in 70% ethanol or 10% sodium hypochlorite. After 30 min at 23 degrees C, spumavirus infectivity in normal but not heat-inactivated human serum increased by almost 100-fold. Persistence of infectivity of primate spumavirus after 7 days in media and waters, and the agent's infectious potential in the human host, emphasize a need for cautious recognition during the manipulation of primate cells/organs and in the handling of primates themselves.

In vitro approaches to evaluate placental drug transport by using differentiating JEG-3 human choriocarcinoma cells.

PubMed

Ikeda, Kenji; Utoguchi, Naoki; Tsutsui, Hidenobu; Yamaue, Satoko; Homemoto, Manami; Nakao, Erina; Hukunaga, Yumi; Yamasaki, Kyohei; Myotoku, Michiaki; Hirotani, Yoshihiko

2011-02-01

Human choriocarcinoma cells have been used as models for studying transcellular drug transport through placental trophoblasts. However, these models allow the transport of low-molecular-weight drugs through intercellular gap junctions. This study aimed at investigating the differentiation patterns of JEG-3 choriocarcinoma cells under different culture conditions and establishing the appropriate model of in vitro syncytiotrophoblast drug transport. Paracellular permeability was estimated by measuring the transepithelial electrical resistance (TEER) across JEG-3 cell layers. The mRNA expression levels of non-expressed in choriocarcinoma clone 1 (NECC1) and breast cancer resistance protein (BCRP), and those of E-cadherin (ECAD) and cadherin-11 (CDH11), which are adherens junction-associated proteins related to fusogenic ability of syncytiotrophoblasts differentiated from cytotrophoblasts, protein expression levels were considered as the differentiation signals. The highest TEER values were obtained in the JEG-3 cells cultured in the Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 (1:1) mixed medium (CS-C(®) ; Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan). By comparing the TEER values and the differentiation signals, the authors identified at least five JEG-3 cell-differentiation patterns. The differentiation pattern of JEG-3 cultured in CS-C resembled the syncytiotrophoblast-like differentiation signal characterizations in vivo. In conclusion, the syncytiotrophoblast-like models of differentiating JEG-3 cells cultured in CS-C might be appropriate for evaluating drug transport across the placental trophoblast. © 2010 The Authors. Basic & Clinical Pharmacology & Toxicology © 2010 Nordic Pharmacological Society.

Establishment of a hepatocyte line for studying biosynthesis of long-chain polyunsaturated fatty acids from a marine teleost, the white-spotted spinefoot Siganus canaliculatus.

PubMed

Liu, Y; Zhang, Q H; Dong, Y W; You, C H; Wang, S Q; Li, Y Q; Li, Y Y

2017-08-01

A hepatocyte line was established from the liver of white-spotted spinefoot Siganus canaliculatus to study the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA). The cells from the line, designated S. canaliculatus hepatocyte line (SCHL), grew and multiplied well in Dulbecco's modified Eagle's medium (DMEM)-F12 medium supplemented with 20 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulphonic acid (HEPES), 10% foetal bovine serum (FBS) and 0·5% rainbow trout Oncorhychus mykiss serum at 28° C, showing an epithelial-like morphology and the normal chromosome number of 48 (2n) and have been subcultured for over 60 passages. The identity of the hepatocytes was confirmed by periodic acid Schiff (PAS) staining. The mRNA expression of all genes encoding the key enzymes for LC-PUFA biosynthesis including two desaturases (Δ4 Fad and Δ6-Δ5 Fad) and two elongases (Elovl4 and Elovl5), were detected in all cells from passages 5 to 60 and their expression levels became stable after passage 35 and showed responses to various PUFA incubation. This is similar to the situation determined in the liver of S. canaliculatus that were fed diets containing different fatty acids. These results indicated that SCHL was successfully established and can provide an in vitro tool to investigate lipid metabolism and regulatory mechanisms of LC-PUFA biosynthesis in teleosts, especially marine species. © 2017 The Fisheries Society of the British Isles.

In vitro biocompatibility of nickel-titanium esthetic orthodontic archwires.

PubMed

Rongo, Roberto; Valletta, Rosa; Bucci, Rosaria; Rivieccio, Virginia; Galeotti, Angela; Michelotti, Ambrosina; D'Antò, Vincenzo

2016-09-01

To investigate the cytotoxicity of nickel-titanium (NiTi) esthetic orthodontic archwires with different surface coatings. Three fully coated, tooth-colored NiTi wires (BioCosmetic, Titanol Cosmetic, EverWhite), two ion-implanted wires (TMA Purple, Sentalloy High Aesthetic), five uncoated NiTi wires (BioStarter, BioTorque, Titanol Superelastic, Memory Wire Superelastic, and Sentalloy), one β-titanium wire (TMA), and one stainless steel wire (Stainless Steel) were considered for this study. The wire samples were placed at 37°C in airtight test tubes containing Dulbecco's Modified Eagle's Medium (0.1 mg/mL) for 1, 7, 14, and 30 days. The cell viability of human gingival fibroblasts (HGFs) cultured with this medium was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data were analyzed by a two-way analysis of variance (α  =  .05). The highest cytotoxic effect was reached on day 30 for all samples. The archwires exhibited a cytotoxicity on HGFs ranging from "none" to "slight," with the exception of the BioTorque, which resulted in moderate cytotoxicity on day 30. Significant differences were found between esthetic archwires and their uncoated pairs only for BioCosmetic (P  =  .001) and EverWhite (P < .001). Under the experimental conditions, all of the NiTi esthetic archwires resulted in slight cytotoxicity, as did the respective uncoated wires. For this reason their clinical use may be considered to have similar risks to the uncoated archwires.

Agonistic asymmetries and the foraging ecology of Bald Eagles

USGS Publications Warehouse

Knight, Richard L.; Skagen, Susan Knight

1988-01-01

We investigated the effects of both asymmetries and differing food levels on contest outcomes of wintering Bald Eagles (Haliaeetus leucocephalus) feeding on chum salmon (Oncorhynchus keta) carcasses. Large eagles, regardless of age, were more successful in pirating than smaller eagles. Small pirating eagles were usually unsuccessful unless they were adults attempting to supplant other small eagles. Feeding eagles were more successful in defeating pirating eagles according to (1) whether their heads were up to prior to a pirating attempt, (2) how long their heads had been up, and (3) whether they displayed. During periods of food scarcity pirating eagles were less successful, a fact attributed in a proximate sense to the increase incidence of retaliation by feeding birds. When food was scarce and eagles had a choice between scavenging the pirating, they chose to scavenge more often. Body size appears to be an important factor in determining social dominance and influencing differences in foraging modes of wintering Bald Eagles.

Cell Growth Arrest Mediated by STAT Proteins in Breast Cancer Cells

DTIC Science & Technology

1998-07-01

serum, PC 12 rat adrenal pheochromocytoma cells (ATCC, CRL-1721) in DMEM containing 10 % fetal bovine serum, 10 % horse serum, and 1 raM L-glutamine... unusual . Additionally, we have mostly presented the biochemical data showing this inhibitor activity. What is in vivo implication of the STAT...PC12 rat adrenal pheochromocytoma cells (ATCC, CRL-1721) in Dulbecco’s mod- ified Eagle’s medium containing 10% fetal bovine serum, 10% horse

Effect of 1,25-dihydroxyvitamin D3 on human keratinocytes grown under different culture conditions.

PubMed

McLane, J A; Katz, M; Abdelkader, N

1990-04-01

1,25-Dihydroxyvitamin D3 (1,25-(OH)2-D3) is known to decrease the proliferation and increase the differentiation of different cell types including human keratinocytes. The growth and differentiation of keratinocytes in the presence of 1,25-(OH)2-D3 using serum-free media formulations has been described previously. This investigation extends these studies to describe various culture conditions with human foreskin keratinocytes to determine the optimal antiproliferative activity of 1,25-(OH)2-D3. Keratinocytes were plated onto tissue culture dishes using one of three basic serum-free media protocols; a) with no feeder layer in keratinocyte growth medium (KGM); b) onto mitomycin C-treated 3T3 mouse embryo fibroblasts; or c) onto mitomycin C-treated dermal human fibroblasts. The last two protocols utilized Dulbecco's modified Eagle's Medium (DMEM) supplemented with growth factors. Keratinocyte cell growth was greatest in the KGM medium. Although the growth of keratinocytes on either feeder layer was similar, there were differences in the ability of the cells to form envelopes in the presence of 1,25-(OH)2-D3. The addition of hydrocortisone and cholera toxin to the medium also affected the response of the keratinocytes to 1,25-(OH)2-D3. The antiproliferative effect of 1,25-(OH)2-D3 was not altered by varying the extracellular calcium levels from 0.25 to 3 mM. The antiproliferative activity of 1,25-(OH)2-D3 is attenuated in cells at low density. Our results suggest that an optimal condition to investigate the ability of 1,25-(OH)2-D3 to inhibit keratinocyte proliferation is at preconfluent cell density in the presence of KGM supplemented with 1.5 mM calcium without a feeder layer. These conditions are not appropriate for investigating the enhancement of differentiation by 1,25-(OH)2-D3, but can be used to assay other agents that modulate keratinocyte proliferation.

Establishment of a rat hepatoma-derived cell line proliferating in D-phenylalanine medium and expressing D-amino-acid oxidase.

PubMed

Yoda, N; Konno, R; Nagashima, S

2001-01-01

A cell line (R-Y121B.DF) has been established from a cell line (R-Y121B) derived from a rat hepatoma line (H4-II-E). The R-Y121B.DF cells have been continuously cultured in a serum-free modified Eagle's minimum essential medium in which L-phenylalanine was replaced by D-phenylalanine. They had D-amino-acid oxidase (DAO) activity which is essential for the growth in the medium containing D-amino acids. The enzyme activity of the R-Y121B.DF cells was approximately one-fourth of that of the rat liver. Northern hybridization using a DAO cDNA probe detected a hybridizing signal in the R-Y121B.DF cells and the rat liver but not in the parental R-Y121B and H4-II-E cells. Reverse transcription-polymerase chain reaction using DAO-specific primers amplified a DNA fragment of the expected size in the R-Y121B.DF cells but not in the R-Y121B and H4-II-E cells. This fragment was confirmed to be DAO cDNA by nucleotide sequencing. Western blotting showed that DAO protein was present in the R-Y121B.DF cells and the rat liver but not in the R-Y121B and H4-II-E cells. Southern hybridization showed that the DAO gene structure was not different among the R-Y121B.DF cells, R-Y121B cells, H4-II-E cells, and the rat liver. These results indicate that the R-Y121B.DF is a unique cell line which proliferates in the medium containing D-phenylalanine and explicitly expresses DAO. This line is useful for the study of DAO in vitro.

Lead and eagles: demographic and pathological characteristics of poisoning, and exposure levels associated with other causes of mortality

USGS Publications Warehouse

Franson, J. Christian; Russell, Robin E.

2014-01-01

We conducted a retrospective analysis to evaluate demographic and pathologic characteristics in 484 bald eagles (Haliaeetus leucocephalus) and 68 golden eagles (Aquila chrysaetos) diagnosed with lead poisoning at the U.S. Geological Survey National Wildlife Health Center. As part of our analysis, we compared characteristics of lead poisoned eagles with those that died of other causes. Odds of lead poisoning were greater for bald eagles versus golden eagles, females versus males, adults versus juveniles, and eagles from the Mississippi and Central flyways versus the Atlantic and Pacific flyways. In addition to spatial, species, and demographic associations, we detected a distinct temporal trend in the collection date of lead poisoned bald eagle carcasses. These carcasses were found at greater frequency in late autumn and winter than spring and summer. Lesions in lead poisoned birds included emaciation, evidence of bile stasis, myocardial degeneration and necrosis, and renal tubular nephrosis and necrosis. Ingested lead ammunition or fragments were found in 14.2 % of bald eagles and 11.8 % of golden eagles. The overall mean liver lead concentration (wet weight basis) for eagles diagnosed with lead poisoning was 28.9 ± 0.69 SE mg/kg in bald eagles and 19.4 ± 1.84 SE mg/kg in golden eagles. In eagles diagnosed with collision trauma, electrocution, poisoning (other than lead), emaciation, infectious disease, trapping death, other, and undetermined causes, average liver lead concentrations were low (<1 mg/kg) and did not differ among causes of mortality. Thus, based on our data, we found no evidence that lead exposure of eagles predisposed them to other causes of mortality.

Baseflow recession analysis across the Eagle Ford shale play (Texas, USA)

NASA Astrophysics Data System (ADS)

Arciniega, Saul; Brena-Naranjo, Agustin; Hernandez-Espriu, Jose Antonio; Pedrozo-Acuña, Adrian

2016-04-01

Baseflow is an important process of the hydrological cycle as it can be related to aquatic ecosystem health and groundwater recharge. The temporal and spatial dynamics of baseflow are typically governed by fluctuations in the water table of shallow aquifers hence groundwater pumping and return flow can greatly modify baseflow patterns. More recently, in some regions of the world the exploitation of gas trapped in shale formations by means of hydraulic fracturing (fracking) has raised major concerns on the quantitative and qualitative groundwater impacts. Although fracking implies massive amounts of groundwater withdrawals, its contribution on baseflow decline has not yet been fully investigated. Furthermore, its impact with respect to other human activities or climate extremes such as irrigation or extreme droughts, respectively, remain largely unknown. This work analyzes baseflow recession time-space patterns for a set of watersheds located across the largest shale producer in the world, the Eagle Ford shale play in Texas (USA). The period of study (1985-2014) includes a pre-development and post-development period. The dataset includes 56 hydrometric time series located inside and outside the shale play. Results show that during the development and expansion of the Eagle Ford play, around 70 % of the time series displayed a significant decline wheras no decline was observed during the pre-development)

Investigations on in vitro anti-carcinogenic potential of L-carnosine in liver cancer cells.

PubMed

Ding, Minghui; Jiao, Guihua; Shi, Haizhou; Chen, Yanrong

2018-02-01

This study was carried out to investigate the anti-carcinogenic effect of L-carnosine in human carcinoma cells (SNU-423). The SNU-423 cancer cells were cultured at a density of 2 × 10 4 cells/well in Dulbecco modified Eagle medium. After 24 h of adherence, the cells were treated with L-carnosine (0.2 and 1 mg/mL) for 48 h. Then, cell viability was assessed by sulforhodamine assay, while mitochondrial dysfunction was measured by fluorescence microscopy using chromatin-specific dye Hoechst 33258. Intracellular levels of ROS were assayed by fluorescence spectroscopy with 2',7'-dichlorofluorescein diacetate (DCFDA). L-Carnosine significantly inhibited the growth of the SNU-423 cells (p < 0.05). The inhibitory effect of L-carnosine was confirmed by results from mitochondrial fragmentation assay. The relative fluorescent unit was increased in a dose-dependent manner by L-carnosine, with values of 79.43, 186.87 and 400.89 for 0.6, 0.8 and 1 mg/mL of L-carnosine, respectively (p < 0.05). These results demonstrate that L-carnosine exerts anti-carcinogenic effects in human liver cancer cells.

Highly sensitive three-dimensional interdigitated microelectrode for microparticle detection using electrical impedance spectroscopy

NASA Astrophysics Data System (ADS)

Chang, Fu-Yu; Chen, Ming-Kun; Wang, Min-Haw; Jang, Ling-Sheng

2016-02-01

Cell impedance analysis is widely used for monitoring biological and medical reactions. In this study, a highly sensitive three-dimensional (3D) interdigitated microelectrode (IME) with a high aspect ratio on a polyimide (PI) flexible substrate was fabricated for microparticle detection (e.g. cell quantity detection) using electroforming and lithography technology. 3D finite element simulations were performed to compare the performance of the 3D IME (in terms of sensitivity and signal-to-noise ratio) to that of a planar IME for particles in the sensing area. Various quantities of particles were captured in Dulbecco’s modified Eagle medium and their impedances were measured. With the 3D IME, the particles were arranged in the gap, not on the electrode, avoiding the noise due to particle position. For the maximum particle quantities, the results show that the 3D IME has at least 5-fold higher sensitivity than that of the planar IME. The trends of impedance magnitude and phase due to particle quantity were verified using the equivalent circuit model. The impedance (1269 Ω) of 69 particles was used to estimate the particle quantity (68 particles) with 98.6% accuracy using a parabolic regression curve at 500 kHz.

Enhanced corrosion resistance and hemocompatibility of biomedical NiTi alloy by atmospheric-pressure plasma polymerized fluorine-rich coating

NASA Astrophysics Data System (ADS)

Li, Penghui; Li, Limin; Wang, Wenhao; Jin, Weihong; Liu, Xiangmei; Yeung, Kelvin W. K.; Chu, Paul K.

2014-04-01

To improve the corrosion resistance and hemocompatibility of biomedical NiTi alloy, hydrophobic polymer coatings are deposited by plasma polymerization in the presence of a fluorine-containing precursor using an atmospheric-pressure plasma jet. This process takes place at a low temperature in air and can be used to deposit fluoropolymer films using organic compounds that cannot be achieved by conventional polymerization techniques. The composition and chemical states of the polymer coatings are characterized by fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The corrosion behavior of the coated and bare NiTi samples is assessed and compared by polarization tests and electrochemical impedance spectroscopy (EIS) in physiological solutions including simulated body fluids (SBF) and Dulbecco's Modified Eagle's medium (DMEM). The corrosion resistance of the coated NiTi alloy is evidently improved. Protein adsorption and platelet adhesion tests reveal that the adsorption ratio of albumin to fibrinogen is increased and the number of adherent platelets on the coating is greatly reduced. The plasma polymerized coating renders NiTi better in vitro hemocompatibility and is promising as a protective and hemocompatible coating on cardiovascular implants.

Anionic clay as the drug delivery vehicle: tumor targeting function of layered double hydroxide-methotrexate nanohybrid in C33A orthotopic cervical cancer model.

PubMed

Choi, Goeun; Piao, Huiyan; Alothman, Zeid A; Vinu, Ajayan; Yun, Chae-Ok; Choy, Jin-Ho

2016-01-01

Methotrexate (MTX), an anticancer agent, was successfully intercalated into the anionic clay, layered double hydroxides to form a new nanohybrid drug. The coprecipitation and subsequent hydrothermal method were used to prepare chemically, structurally, and morphologically well-defined two-dimensional drug-clay nanohybrid. The resulting two-dimensional drug-clay nanohybrid showed excellent colloidal stability not only in deionized water but also in an electrolyte solution of Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum, in which the average particle size in colloid and the polydispersity index were determined to be around 100 and 0.250 nm, respectively. The targeting property of the nanohybrid drug was confirmed by evaluating the tumor-to-blood and tumor-to-liver ratios of the MTX with anionic clay carrier, and these ratios were compared to those of free MTX in the C33A orthotopic cervical cancer model. The biodistribution studies indicated that the mice treated with the former showed 3.5-fold higher tumor-to-liver ratio and fivefold higher tumor-to-blood ratio of MTX than those treated with the latter at 30 minutes postinjection.

77 FR 27174 - Eagle Permits; Changes in the Regulations Governing Eagle Permitting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

..., proposed rule to revise the regulations for permits for nonpurposeful take of golden eagles (Aquila... will post all comments on http:/www.regulations.gov. This generally means that we will post any... of golden eagles (Aquila chrysaetos) and bald eagles (Haliaeetus leucocephalus), where the take is...

77 FR 47628 - Eagle Mountain Pumped Storage Hydroelectric Project; Eagle Crest Energy; Notice of Meeting...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-13123-002-CA] Eagle Mountain Pumped Storage Hydroelectric Project; Eagle Crest Energy; Notice of Meeting Postponement On July 17, 2012, the...), on the Eagle Mountain Pumped Storage Hydroelectric Project. However, the meeting has been postponed...

Staining of retinal neurons in the isolated eyecup by extracellular horseradish peroxidase injection.

PubMed

Amthor, F R; Jackson, C A

1986-01-01

A reliable method has been developed for the staining of mammalian retinal neurons in the isolated eyecup. Small injections of horseradish peroxidase are made at a number of places on the retinal surface while the hemisected eyecup preparation floats in Eagle's medium. After 30 min, the retinas are removed, fixed, and reacted with diaminobenzidine. Golgi-like staining of fine dendrites, spines and axons of ganglion cells is achieved. The method also stains amacrine, horizontal and bipolar cells.

Golden eagle records from the Midwinter Bald Eagle Survey: information for wind energy management and planning

USGS Publications Warehouse

Eakle, Wade; Haggerty, Patti; Fuller, Mark; Phillips, Susan L.

2013-01-01

The purpose of this Data Series report is to provide the occasions, locations, and counts when golden eagles were recorded during the annual Midwinter Bald Eagle Surveys. Golden eagles (Aquila chrysaetos) are protected by Federal statutes including the Bald and Golden Eagle Protection Act (BGEPA) (16 USC 668-668c) and the Migratory Bird Treaty Act (MBTA) (16 USC 703-12). The U.S. Fish and Wildlife Service (Service) manages golden eagles with the goal of maintaining stable or increasing breeding populations (U.S. Fish and Wildlife Service, 2009). Development for the generation of electricity from wind turbines is occurring in much of the range of the golden eagle in the western United States. Development could threaten population stability because golden eagles might be disturbed by construction and operation of facilities and they are vulnerable to mortality from collisions with wind turbines (Smallwood and Thelander, 2008). Therefore, the Service has proposed a process by which wind energy developers can collect information that could lead to Eagle Conservation Plans (ECP), mitigation, and permitting that allow for golden eagle management in areas of wind energy development (U.S. Fish and Wildlife Service, 2011). The Service recommends that ECP be developed in stages, and the first stage is to learn if golden eagles occur at the landscape level where potential wind facilities might be located. Information about where eagles occur can be obtained from technical literature, agency files, and other sources of information including on-line biological databases. The broad North American distribution of golden eagles is known, but there is a paucity of readily available information about intermediate geographic scales and site-specific scales, especially during the winter season (Kochert and others, 2002).

Relationship of diets and environmental contaminants in wintering bald eagles. [Haliaeetus leucocephalus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Frenzel, R.W.; Anthony, R.G.

1989-07-01

We investigated the relationship between diets and potential hazards in contaminants of wintering bald eagles (Haliaeetus leucocephalus) in the Klamath Basin of northern California and southern Oregon. We studied diets by identifying remains of 913 prey items found at perches, examining 341 castings collected from communal night roots, and observing foraging eagles. We determined residues of organochlorine compounds, lead (Pb), and mercury (Hg) in bald eagles and their prey by analyzing eagle blood samples and carcasses and 8 major prey species. Bald eagles fed largely on waterfowl by scavenging cholera-killed ducks and geese and on microtine rodents during mid- tomore » late winter. Residues of organochlorine pesticides and Hg in prey were low, and polychlorinated biphenyls (PCB's) were detected in low concentrations in 9% of prey samples. Means Pb concentrations in prey ranged from 0.15 to 4.79 ppm. Mercury was detected in all eagle blood samples, and Pb was detected in 41% of the bald eagle blood samples. Mean Pb concentration in livers of dead eagles was 2.09 ppm and ranged as high as 27 ppm in an eagle that died of Pb poisoning. Prey of the eagles were relatively free of contaminants with the possible exception of embedded Pb shot in waterfowl, which may present a potential for Pb poisoning of eagles.« less

76 FR 65507 - Notice of Petition for Rate Approval; Eagle Ford Midstream, LP

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... Petition for Rate Approval; Eagle Ford Midstream, LP Take notice that on October 11, 2011, (Eagle Ford..., and its initial baseline Statement of Operating Conditions. Eagle Ford states that it is an existing..., currently providing intrastate services to its customers. Eagle Ford proposed rates for Section 311...

36 CFR 71.5 - Golden Eagle Passport.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 1 2013-07-01 2013-07-01 false Golden Eagle Passport. 71.5... RECREATION FEES § 71.5 Golden Eagle Passport. (a) The Golden Eagle Passport is an annual permit, valid on a... Passport shall be $10. The annual Golden Eagle Passport shall be nontransferable and the unlawful use...

36 CFR 71.5 - Golden Eagle Passport.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Golden Eagle Passport. 71.5... RECREATION FEES § 71.5 Golden Eagle Passport. (a) The Golden Eagle Passport is an annual permit, valid on a... Passport shall be $10. The annual Golden Eagle Passport shall be nontransferable and the unlawful use...

36 CFR 71.5 - Golden Eagle Passport.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 1 2014-07-01 2014-07-01 false Golden Eagle Passport. 71.5... RECREATION FEES § 71.5 Golden Eagle Passport. (a) The Golden Eagle Passport is an annual permit, valid on a... Passport shall be $10. The annual Golden Eagle Passport shall be nontransferable and the unlawful use...

36 CFR 71.5 - Golden Eagle Passport.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 36 Parks, Forests, and Public Property 1 2012-07-01 2012-07-01 false Golden Eagle Passport. 71.5... RECREATION FEES § 71.5 Golden Eagle Passport. (a) The Golden Eagle Passport is an annual permit, valid on a... Passport shall be $10. The annual Golden Eagle Passport shall be nontransferable and the unlawful use...

36 CFR 71.5 - Golden Eagle Passport.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 36 Parks, Forests, and Public Property 1 2011-07-01 2011-07-01 false Golden Eagle Passport. 71.5... RECREATION FEES § 71.5 Golden Eagle Passport. (a) The Golden Eagle Passport is an annual permit, valid on a... Passport shall be $10. The annual Golden Eagle Passport shall be nontransferable and the unlawful use...

Lead exposure in bald eagles from big game hunting, the continental implications and successful mitigation efforts.

PubMed

Bedrosian, Bryan; Craighead, Derek; Crandall, Ross

2012-01-01

Studies suggest hunter discarded viscera of big game animals (i.e., offal) is a source of lead available to scavengers. We investigated the incidence of lead exposure in bald eagles in Wyoming during the big game hunting season, the influx of eagles into our study area during the hunt, the geographic origins of eagles exposed to lead, and the efficacy of using non-lead rifle ammunition to reduce lead in eagles. We tested 81 blood samples from bald eagles before, during and after the big game hunting seasons in 2005-2010, excluding 2008, and found eagles had significantly higher lead levels during the hunt. We found 24% of eagles tested had levels indicating at least clinical exposure (>60 ug/dL) during the hunt while no birds did during the non-hunting seasons. We performed driving surveys from 2009-2010 to measure eagle abundance and found evidence to suggest that eagles are attracted to the study area during the hunt. We fitted 10 eagles with satellite transmitters captured during the hunt and all migrated south after the cessation of the hunt. One returned to our study area while the remaining nine traveled north to summer/breed in Canada. The following fall, 80% returned to our study area for the hunting season, indicating that offal provides a seasonal attractant for eagles. We fitted three local breeding eagles with satellite transmitters and none left their breeding territories to feed on offal during the hunt, indicating that lead ingestion may be affecting migrants to a greater degree. During the 2009 and 2010 hunting seasons we provided non-lead rifle ammunition to local hunters and recorded that 24% and 31% of successful hunters used non-lead ammunition, respectively. We found the use of non-lead ammunition significantly reduced lead exposure in eagles, suggesting this is a viable solution to reduce lead exposure in eagles.

Lead Exposure in Bald Eagles from Big Game Hunting, the Continental Implications and Successful Mitigation Efforts

PubMed Central

Bedrosian, Bryan; Craighead, Derek; Crandall, Ross

2012-01-01

Studies suggest hunter discarded viscera of big game animals (i.e., offal) is a source of lead available to scavengers. We investigated the incidence of lead exposure in bald eagles in Wyoming during the big game hunting season, the influx of eagles into our study area during the hunt, the geographic origins of eagles exposed to lead, and the efficacy of using non-lead rifle ammunition to reduce lead in eagles. We tested 81 blood samples from bald eagles before, during and after the big game hunting seasons in 2005–2010, excluding 2008, and found eagles had significantly higher lead levels during the hunt. We found 24% of eagles tested had levels indicating at least clinical exposure (>60 ug/dL) during the hunt while no birds did during the non-hunting seasons. We performed driving surveys from 2009–2010 to measure eagle abundance and found evidence to suggest that eagles are attracted to the study area during the hunt. We fitted 10 eagles with satellite transmitters captured during the hunt and all migrated south after the cessation of the hunt. One returned to our study area while the remaining nine traveled north to summer/breed in Canada. The following fall, 80% returned to our study area for the hunting season, indicating that offal provides a seasonal attractant for eagles. We fitted three local breeding eagles with satellite transmitters and none left their breeding territories to feed on offal during the hunt, indicating that lead ingestion may be affecting migrants to a greater degree. During the 2009 and 2010 hunting seasons we provided non-lead rifle ammunition to local hunters and recorded that 24% and 31% of successful hunters used non-lead ammunition, respectively. We found the use of non-lead ammunition significantly reduced lead exposure in eagles, suggesting this is a viable solution to reduce lead exposure in eagles. PMID:23284837

MQ-1C Gray Eagle Unmanned Aircraft System (MQ-1C Gray Eagle)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-420 MQ-1C Gray Eagle Unmanned Aircraft System (MQ-1C Gray Eagle) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 21, 2016 17:33:19 UNCLASSIFIED MQ-1C Gray Eagle December 2015 SAR March 21...Gray Eagle December 2015 SAR March 21, 2016 17:33:19 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM

76 FR 15971 - Eagle Mountain Pumped Storage Hydroelectric Project; Eagle Crest Energy; Notice of Teleconference

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-22

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-13123-002-CA] Eagle Mountain Pumped Storage Hydroelectric Project; Eagle Crest Energy; Notice of Teleconference a. Date and Time of Meeting... staff of the U.S. Fish and Wildlife Service and Eagle Crest Energy as part of its on-going Section 7...

76 FR 22699 - Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Teleconference

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-22

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-13123-002--CA] Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Teleconference a. Date and Time of Meeting... staff of the U.S. Fish and Wildlife Service and Eagle Crest Energy as part of its on-going Section 7...

77 FR 61661 - Price for the American Eagle Silver Proof and Uncirculated Coins and the America the Beautiful...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... DEPARTMENT OF THE TREASURY United States Mint Price for the American Eagle Silver Proof and... price of silver, the United States Mint is raising the price of its American Eagle Silver Proof and... price 2012 American Eagle Silver Proof $59.95 2012 American Eagle Silver Uncirculated 50.95 2011...

Biotelemetery data for golden eagles (Aquila chrysaetos) captured in coastal southern California, February 2016–February 2017

USGS Publications Warehouse

Tracey, Jeff A.; Madden, Melanie C.; Sebes, Jeremy B.; Bloom, Peter H.; Katzner, Todd E.; Fisher, Robert N.

2017-05-12

Because of a lack of clarity about the status of golden eagles (Aquila chrysaetos) in coastal southern California, the USGS, in collaboration with local, State, and other Federal agencies, began a multi-year survey and tracking program of golden eagles to address questions regarding habitat use, movement behavior, nest occupancy, genetic population structure, and human impacts on eagles. Golden eagle trapping and tracking efforts began in September 2014. During trapping efforts from September 29, 2014, to February 23, 2016, 27 golden eagles were captured. During trapping efforts from February 24, 2016, to February 23, 2017, an additional 10 golden eagles (7 females and 3 males) were captured in San Diego, Orange, and western Riverside Counties. Biotelemetry data for 26 of the 37 golden eagles that were transmitting data from February 24, 2016, to February 23, 2017 are presented. These eagles ranged as far north as northern Nevada and southern Wyoming, and as far south as La Paz, Baja California, Mexico.

Socio-Culturally Oriented Plan Discovery Environment (SCOPE)

DTIC Science & Technology

2005-05-01

U.S. intelligence methods (Dr. George Friedman ( 2003 ) Saddam Hussein and the Dollar War. THE STRATFOR WEEKLY 18 December) 8 2.2. Evidence... 2003 ). In the EAGLE setting, we are using a modified version of the fuzzy segmentation algorithm developed by Udupa and his associates to...based (Fu, et al, 2003 ) and a cognitive model based (Eilbert, et al., 2002) algorithms, and a method for combining the results. (The method for

75 FR 56093 - Eagle Rock Desoto Pipeline, LP; Notice of Motion for Extension of Rate Case Filing Deadline

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-15

... pipelines to extend the cycle for such reviews from three to five years.\\1\\ Therefore, Eagle Rock requests... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR09-1-002] Eagle Rock... notice that on September 8, 2010, Eagle Rock Desoto Pipeline, L.P. (Eagle Rock) filed a request to extend...

Annual movements of a steppe eagle (Aquila nipalensis) summering in Mongolia and wintering in Tibet

USGS Publications Warehouse

Ellis, D.H.; Moon, S.L.; Robinson, J.W.

2001-01-01

An adult female steppe eagle (Aquila nipalensis Hodgson) was captured and fitted with a satellite transmitter in June 1995 in southeastern Mongolia. In fall, it traveled southwest towards India as expected, but stopped in southeastern Tibet and wintered in a restricted zone within the breeding range of the steppe eagle. In spring, the bird returned to the same area of Mongolia where it was captured. These observations, though derived from the movements of a single bird, suggest three things that are contrary to what is generally believed about steppe eagle biology. First, not all steppe eagles move to warmer climes in winter. Second, not all steppe eagles are nomadic in winter. Finally, because our bird wintered at the periphery of the steppe eagle breeding range in Tibet, perhaps birds that breed in this same area also winter there. If so, not all steppe eagles are migratory.

Organochlorine pesticide, PCB, and PBB residues and necropsy data for bald eagles from 29 states - 1975-77

USGS Publications Warehouse

Kaiser, T. Earl; Reichel, William L.; Locke, Louis N.; Cromartie, Eugene; Krynitsky, Alexander J.; Lamont, Thair; Mulhern, Bernard M.; Prouty, Richard M.; Stafford, Charles J.; Swineford, Douglas M.

1980-01-01

During 1975-77, 168 bald eagles (Haliaeetus leucocephalus) found moribund or dead in 29 states were analyzed for organochlorine pesticides and polychlorinated biphenyls (PCBs); 32 specimens from 13 states were analyzed for polybrominated biphenyls (PBBs). PCBs were present in 166 bald eagle carcasses and DDE was found in 165. TDE and dieldrin were identified in 137 samples, trans-nonachlor in 118, and oxychlordane in 90. Brains of five eagles contained possible lethal levels of dieldrin, and two eagles possibly died of endrin poisoning. Nine eagle livers, analyzed because of suspected lead poisoning, contained high levels of lead. Twenty percent of the eagles died from shooting, the most common cause of death; this cause of death, however, has declined.

The Investigation of ADAMTS16 in Insulin-Induced Human Chondrosarcoma Cells.

PubMed

Cakmak, Ozlem; Comertoglu, Ismail; Firat, Ridvan; Erdemli, Haci Kemal; Kursunlu, S Fatih; Akyol, Sumeyya; Ugurcu, Veli; Altuntas, Aynur; Adam, Bahattin; Demircan, Kadir

2015-08-01

A disintegrin-like metalloproteinase with thrombospondin motifs (ADAMTS) is a group of proteins that have enzymatic activity secreted by cells to the outside extracellular matrix. Insulin induces proteoglycan biosynthesis in chondrosarcoma chondrocytes. The purpose of the present in vitro study is to assess the time course effects of insulin on ADAMTS16 expression in OUMS-27 (human chondrosarcoma) cell line to examine whether insulin regulates ADAMTS16 expression as well as proteoglycan biosynthesis with multifaceted properties or not. Chondrosarcoma cells were cultured in Dulbecco's modified Eagle's medium having either 10 μg/mL insulin or not. While the experiment was going on, the medium containing insulin had been changed every other day. Cells were harvested at 1st, 3rd, 7th, and 11th days; subsequently, RNA and proteins were isolated in every experimental group according to their time interval. RNA expression of ADAMTS was estimated by quantitative real-time polymerase chain reaction (qRT-PCR) by using primers. Immunoreactive protein levels were encountered by the western blot protein detection technique by using proper anti-ADAMTS16 antibodies. ADAMTS16 mRNA expression level of chondrosarcoma cells was found to be insignificantly decreased in chondrosarcoma cells induced by insulin detected by the qRT-PCR instrument. On the other hand, there was a gradual decrease in immune-reactant ADAMTS16 protein amount by the time course in insulin-treated cell groups when compared with control cells. It has been suggested that insulin might possibly regulate ADAMTS16 levels/activities in OUMS-27 chondrosarcoma cells taking a role in extracellular matrix turnover.

Cisplatin loaded PMMA: mechanical properties, surface analysis and effects on Saos-2 cell culture.

PubMed

Özben, Hakan; Eralp, Levent; Baysal, Gökhan; Cort, Ayşegül; Sarkalkan, Nazli; Özben, Tomris

2013-01-01

Despite wide resection and systemic chemotherapy, bone tumors may present with local recurrences, metastases and pathological fractures. Application of bone cement containing antineoplastic drug to fill the defect after resection of metastatic lesions and to support implants has been suggested to prevent local tumor growth and implant failures. In this study, we aimed to demonstrate the effects of the addition of cisplatin which is a widely used antineoplastic drug for osteosarcoma, on the mechanical properties of bone cement, and to evaluate the cytotoxic effects of eluted cisplatin on Saos-2 cell culture. Two cement samples were prepared by mixing 100 mg and 300 mg of cisplatin powder with 40 g cement powder. The bone cement of the control group did not contain cisplatin. Mechanical analyses included 4-point bending, compression and shear testing. For cytotoxicity analysis, samples were incubated in Dulbecco's Modified Eagle's medium for 15 days. Mediums were applied to Saos-2 cell culture and cell viability was measured. Surface analyses were performed by scanning electron microscope (SEM). The addition of cisplatin did not alter the mechanical properties of bone cement. It was observed that the eluted cisplatin had cytotoxic effects on Saos-2 cells. SEM analyses demonstrated cisplatin granules on the surface of cement samples. Cisplatin maintains its cytotoxic property when released from bone cement without compromising the mechanical stability. Application of cisplatin loaded bone cement may help local control of tumor growth. We believe that our study will shed light on to these new practices for the treatment of bone cancers and will encourage future studies.

Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li Hongzhen; Zhou Jianjun; Miki, Jun

2008-01-01

Understanding prostate stem cells may provide insight into the origin of prostate cancer. Primary cells have been cultured from human prostate tissue but they usually survive only 15-20 population doublings before undergoing senescence. We report here that RC-170N/h/clone 7 cells, a clonal cell line from hTERT-immortalized primary non-malignant tissue-derived human prostate epithelial cell line (RC170N/h), retain multipotent stem cell properties. The RC-170N/h/clone 7 cells expressed a human embryonic stem cell marker, Oct-4, and potential prostate epithelial stem cell markers, CD133, integrin {alpha}2{beta}1{sup hi} and CD44. The RC-170N/h/clone 7 cells proliferated in KGM and Dulbecco's Modified Eagle Medium with 10% fetalmore » bovine serum and 5 {mu}g/ml insulin (DMEM + 10% FBS + Ins.) medium, and differentiated into epithelial stem cells that expressed epithelial cell markers, including CK5/14, CD44, p63 and cytokeratin 18 (CK18); as well as the mesenchymal cell markers, vimentin, desmin; the neuron and neuroendocrine cell marker, chromogranin A. Furthermore the RC170 N/h/clone 7 cells differentiated into multi tissues when transplanted into the sub-renal capsule and subcutaneously of NOD-SCID mice. The results indicate that RC170N/h/clone 7 cells retain the properties of multipotent stem cells and will be useful as a novel cell model for studying the mechanisms of human prostate stem cell differentiation and transformation.« less

Genetics of the connective tissue proteins: Assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybrids*

PubMed Central

Raj, Cholappadi V. Sundar; Church, Robert L.; Klobutcher, Lawrence A.; Ruddle, Frank H.

1977-01-01

Somatic cell hybrids between mouse and human cell lines have been used to identify the specific chromosome that governs the synthesis of type I procollagen. Fourteen hybrid clones and subclones were derived independently from crosses between mouse parents [LM (thymidine kinase-negative) or A9 (hypoxanthine phosphoribosyltransferase-negative)] and human cells (human diploid lung fibroblasts WI-38 or diploid skin fibroblasts GM5, GM17, and GM9). The cultures were labeled with [3H]proline in modified Eagle's medium without serum. Radioactive procollagens were purified from the medium by the method of Church et al. [(1974) J. Mol. Biol. 86, 785-799]. DEAE-cellulose chromatography was used to separate collagen and type I and type III procollagen. Human type I procollagen was assayed by double immunodiffusion analysis with type I procollagen antibodies prepared by immunizing rabbits with purified human type I procollagen. These analyses combined with karyology and isozyme analyses of each hybrid line have produced evidence for the assignment of the gene for human type I procollagen to chromosome 17. A human microcell-mouse hybrid cell line containing only human chromosome 17 was positive for human type I procollagen, lending further support to the assignment of the human type I procollagen gene to chromosome 17. Finally, by using a hybrid line containing only the long arm of human chromosome 17 translocated onto a mouse chromosome, the type I procollagen gene can be assigned more specifically to the long arm of chromosome 17. Images PMID:412188

N-acetylcysteine stimulates protein synthesis in enterocytes independently of glutathione synthesis.

PubMed

Yi, Dan; Hou, Yongqing; Wang, Lei; Long, Minhui; Hu, Shengdi; Mei, Huimin; Yan, Liqiong; Hu, Chien-An Andy; Wu, Guoyao

2016-02-01

Dietary supplementation with N-acetylcysteine (NAC) has been reported to improve intestinal health and treat gastrointestinal diseases. However, the underlying mechanisms are not fully understood. According to previous reports, NAC was thought to exert its effect through glutathione synthesis. This study tested the hypothesis that NAC enhances enterocyte growth and protein synthesis independently of cellular glutathione synthesis. Intestinal porcine epithelial cells were cultured for 3 days in Dulbecco's modified Eagle medium containing 0 or 100 μM NAC. To determine a possible role for GSH (the reduced form of glutathione) in mediating the effect of NAC on cell growth and protein synthesis, additional experiments were conducted using culture medium containing 100 μM GSH, 100 μM GSH ethyl ester (GSHee), diethylmaleate (a GSH-depletion agent; 10 μM), or a GSH-synthesis inhibitor (buthionine sulfoximine, BSO; 20 μM). NAC increased cell proliferation, GSH concentration, and protein synthesis, while inhibiting proteolysis. GSHee enhanced cell proliferation and GSH concentration without affecting protein synthesis but inhibited proteolysis. Conversely, BSO or diethylmaleate reduced cell proliferation and GSH concentration without affecting protein synthesis, while promoting protein degradation. At the signaling level, NAC augmented the protein abundance of total mTOR, phosphorylated mTOR, and phosphorylated 70S6 kinase as well as mRNA levels for mTOR and p70S6 kinase in IPEC-1 cells. Collectively, these results indicate that NAC upregulates expression of mTOR signaling proteins to stimulate protein synthesis in enterocytes independently of GSH generation. Our findings provide a hitherto unrecognized biochemical mechanism for beneficial effects of NAC in intestinal cells.

3. EAGLE ROCK CONTROL CENTER, OPERATIONS CONTROL. AS SYSTEM BECOMES ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. EAGLE ROCK CONTROL CENTER, OPERATIONS CONTROL. AS SYSTEM BECOMES INCREASINGLY AUTOMATED, EAGLE ROCK WILL BECOME MORE AND MORE THE CENTRAL CONTROL SYSTEM OF THE METROPOLITAN WATER DISTRICT. - Eagle Rock Operations Control Center, Pasadena, Los Angeles County, CA

Final Report Bald and Golden Eagle Territory Surveys for the Lawrence Livermore National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fratanduono, M. L.

2014-11-25

Garcia and Associates (GANDA) was contracted by the Lawrence Livermore National Laboratory (LLNL) to conduct surveys for bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) at Site 300 and in the surrounding area out to 10-miles. The survey effort was intended to document the boundaries of eagle territories by careful observation of eagle behavior from selected viewing locations throughout the study area.

The influence of weather on Golden Eagle migration in northwestern Montana

USGS Publications Warehouse

Yates, R.E.; McClelland, B.R.; Mcclelland, P.T.; Key, C.H.; Bennetts, R.E.

2001-01-01

We analyzed the influence of 17 weather factors on migrating Golden Eagles (Aquila chrysaetos) near the Continental Divide in Glacier National Park, Montana, U.S.A. Local weather measurements were recorded at automated stations on the flanks of two peaks within the migration path. During a total of 506 hr of observation, the yearly number of Golden Eagles in autumn counts (1994-96) averaged 1973; spring counts (1995 and 1996) averaged 605 eagles. Mean passage rates (eagles/hr) were 16.5 in autumn and 8.2 in spring. Maximum rates were 137 in autumn and 67 in spring. Using generalized linear modeling, we tested for the effects of weather factors on the number of eagles counted. In the autumn model, the number of eagles increased with increasing air temperature, rising barometric pressure, decreasing relative humidity, and interactions among those factors. In the spring model, the number of eagles increased with increasing wind speed, barometric pressure, and the interaction between these factors. Our data suggest that a complex interaction among weather factors influenced the number of eagles passing on a given day. We hypothesize that in complex landscapes with high topographic relief, such as Glacier National Park, numerous weather factors produce different daily combinations to which migrating eagles respond opportunistically. ?? 2001 The Raptor Research Foundation, Inc.

Causes of mortality in eagles submitted to the National Wildlife Health Center 1975-2013

USGS Publications Warehouse

Russell, Robin E.; Franson, J. Christian

2014-01-01

We summarized the cause of death for 2,980 bald eagles (Haliaeetus leucocephalus) and 1,427 golden eagles (Aquila chrysaetos) submitted to the National Wildlife Health Center in Madison, Wisconsin, USA, for diagnosis between 1975 and the beginning of 2013. We compared the proportion of eagles with a primary diagnosis as electrocuted, emaciated, traumatized, shot or trapped, diseased, poisoned, other, and undetermined among the 4 migratory bird flyways of the United States (Atlantic, Mississippi, Central, and Pacific). Additionally, we compared the proportion of lead-poisoned bald eagles submitted before and after the autumn 1991 ban on lead shot for waterfowl hunting. Trauma and poisonings (including lead poisoning) were the leading causes of death for bald eagles throughout the study period, and a greater proportion of bald eagles versus golden eagles were diagnosed as poisoned. For golden eagles, the major causes of mortality were trauma and electrocution. The proportion of lead poisoning diagnoses for bald eagles submitted to the National Wildlife Health Center displayed a statistically significant increase in all flyways after the autumn 1991 ban on the use of lead shot for waterfowl hunting. Thus, lead poisoning was a significant cause of mortality in our necropsied eagles, suggesting a continued need to evaluate the trade-offs of lead ammunition for use on game other than waterfowl versus the impacts of lead on wildlife populations. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Polybrominated diphenyl ethers in bald (Haliaeetus leucocephalus) and golden (Aquila chrysaetos) eagles from Washington and Idaho, USA.

PubMed

Spears, Brian Lee; Isanhart, John

2014-12-01

Little is known about the exposure and accumulation of polybrominated diphenyl ethers (PBDEs) in the 2 species of eagles inhabiting North America. The authors analyzed the livers of 33 bald eagles and 7 golden eagles collected throughout Washington and Idaho, USA, for 51 PBDE congeners. Total PBDEs ranged from 2.4 ng/g to 9920 ng/g wet weight. Bald eagles and eagles associated with large urban areas had the highest PBDE concentrations; golden eagles and eagles from more sparsely populated areas had the lowest concentrations. Congener patterns in the present study (brominated diphenyl ether [BDE]-47, BDE-99, BDE-100, BDE-153, and BDE-154 dominating concentrations) were similar to those reported for other bird species, especially raptors. However, the authors also found elevated contributions of BDE-209 in golden eagles and BDE-77 in both species. Patterns in bald eagle samples reflected those in fillets of fish collected from the same general locations throughout Washington, suggesting local exposure to runoff-based contamination, whereas patterns in golden eagle samples suggest a difference in food chain uptake facilitated by atmospheric transport and deposition of BDE-209 and its degradation products into the terrestrial system. Data from the present study represent some of the first reported on burdens of PBDEs in juvenile and adult eagles from North America. The high PBDE liver concentrations associated with large metropolitan areas and accumulation of deca-BDE congeners are a cause for concern. Published 2014 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and as such, is in the public domain in the United States of America.

An adaptive-management framework for optimal control of hiking near golden eagle nests in Denali National Park

USGS Publications Warehouse

Martin, Julien; Fackler, Paul L.; Nichols, James D.; Runge, Michael C.; McIntyre, Carol L.; Lubow, Bruce L.; McCluskie, Maggie C.; Schmutz, Joel A.

2011-01-01

Unintended effects of recreational activities in protected areas are of growing concern. We used an adaptive-management framework to develop guidelines for optimally managing hiking activities to maintain desired levels of territory occupancy and reproductive success of Golden Eagles (Aquila chrysaetos) in Denali National Park (Alaska, U.S.A.). The management decision was to restrict human access (hikers) to particular nesting territories to reduce disturbance. The management objective was to minimize restrictions on hikers while maintaining reproductive performance of eagles above some specified level. We based our decision analysis on predictive models of site occupancy of eagles developed using a combination of expert opinion and data collected from 93 eagle territories over 20 years. The best predictive model showed that restricting human access to eagle territories had little effect on occupancy dynamics. However, when considering important sources of uncertainty in the models, including environmental stochasticity, imperfect detection of hares on which eagles prey, and model uncertainty, restricting access of territories to hikers improved eagle reproduction substantially. An adaptive management framework such as ours may help reduce uncertainty of the effects of hiking activities on Golden Eagles

Modeling climate change impacts on overwintering bald eagles.

PubMed

Harvey, Chris J; Moriarty, Pamela E; Salathé, Eric P

2012-03-01

Bald eagles (Haliaeetus leucocephalus) are recovering from severe population declines, and are exerting pressure on food resources in some areas. Thousands of bald eagles overwinter near Puget Sound, primarily to feed on chum salmon (Oncorhynchus keta) carcasses. We used modeling techniques to examine how anticipated climate changes will affect energetic demands of overwintering bald eagles. We applied a regional downscaling method to two global climate change models to obtain hourly temperature, precipitation, wind, and longwave radiation estimates at the mouths of three Puget Sound tributaries (the Skagit, Hamma Hamma, and Nisqually rivers) in two decades, the 1970s and the 2050s. Climate data were used to drive bald eagle bioenergetics models from December to February for each river, year, and decade. Bald eagle bioenergetics were insensitive to climate change: despite warmer winters in the 2050s, particularly near the Nisqually River, bald eagle food requirements declined only slightly (<1%). However, the warming climate caused salmon carcasses to decompose more rapidly, resulting in 11% to 14% less annual carcass biomass available to eagles in the 2050s. That estimate is likely conservative, as it does not account for decreased availability of carcasses due to anticipated increases in winter stream flow. Future climate-driven declines in winter food availability, coupled with a growing bald eagle population, may force eagles to seek alternate prey in the Puget Sound area or in more remote ecosystems.

Modeling climate change impacts on overwintering bald eagles

PubMed Central

Harvey, Chris J; Moriarty, Pamela E; Salathé Jr, Eric P

2012-01-01

Bald eagles (Haliaeetus leucocephalus) are recovering from severe population declines, and are exerting pressure on food resources in some areas. Thousands of bald eagles overwinter near Puget Sound, primarily to feed on chum salmon (Oncorhynchus keta) carcasses. We used modeling techniques to examine how anticipated climate changes will affect energetic demands of overwintering bald eagles. We applied a regional downscaling method to two global climate change models to obtain hourly temperature, precipitation, wind, and longwave radiation estimates at the mouths of three Puget Sound tributaries (the Skagit, Hamma Hamma, and Nisqually rivers) in two decades, the 1970s and the 2050s. Climate data were used to drive bald eagle bioenergetics models from December to February for each river, year, and decade. Bald eagle bioenergetics were insensitive to climate change: despite warmer winters in the 2050s, particularly near the Nisqually River, bald eagle food requirements declined only slightly (<1%). However, the warming climate caused salmon carcasses to decompose more rapidly, resulting in 11% to 14% less annual carcass biomass available to eagles in the 2050s. That estimate is likely conservative, as it does not account for decreased availability of carcasses due to anticipated increases in winter stream flow. Future climate-driven declines in winter food availability, coupled with a growing bald eagle population, may force eagles to seek alternate prey in the Puget Sound area or in more remote ecosystems. PMID:22822430

Use of noninvasive genetics to assess nest and space use by white-tailed eagles

USGS Publications Warehouse

Bulut, Zafer; Bragin, Evgeny A.; DeWoody, J. Andrew; Braham, Melissa A.; Katzner, Todd E.; Doyle, Jacqueline M.

2016-01-01

Movement and space use are important components of animal interactions with the environment. However, for hard-to-monitor raptor species, there are substantial gaps in our understanding of these key determinants. We used noninvasive genetic tools to evaluate the details of space use over a 3-yr period by White-tailed Eagles (Haliaeetus albicilla) at the Naurzum Zapovednik in northern Kazakhstan. We genotyped, at 10 microsatellite markers and one mitochondrial marker, 859 eagle feathers and assigned naturally shed feathers to individuals. We identified 124 White-tailed Eagles, including both members of 5–10 pairs per year, and were able to monitor birds across years. Distances between eagle nests and hunting perches were always greater than nearest neighbor distances, eagles never used the closest available hunting perch, and hunting perches were always shared with other eagles. When eagles switched nests between years, the nests they chose were almost always well outside the space that theory predicted they defended the prior year. Our data are inconsistent with classical territorial and colonial models of resource use; they more closely resemble semi-colonial behavior. It is unlikely that standard methods of animal tracking (e.g., marking and telemetry), would have provided a similarly cost-effective mechanism to gain these insights into spatial and temporal aspects of eagle behavior. When combined with existing information on space use of other local species, these data suggest that partitioning of spatial resources among White-tailed Eagles and other eagles at the Zapovednik may be facilitated by the alternative strategies of space use they employ.

76 FR 22393 - Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Cancellation...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-21

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-13123-002-CA] Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Cancellation of Teleconference On March 15... Mountain Pumped Storage Hydroelectric Project. This meeting has been cancelled. We will reschedule this...

The Northern Bald Eagle (Haliaeetus leucocephalus alascanus).

DTIC Science & Technology

1979-01-01

finally lured out of the nest with a show of food. Kussman (1976, cited in Diss. Abst. Intern. 38(3):1033-D) studied post- fledging behavior of eagles...Frenzel, L. D., G. Juenemann, and J. Kussman 1973 Behavioral Aspects of Eagle Nest Surveys. pp 33-36 in: Bald Eagle Nest Survey Workshop, 15 Aug. U.S...J. Ligas, and W. B. Robertson, Jr. 1970 Organochlorine and Heavy Metal Residues in Bald Eagle Eggs. Pestic. Monit. Jour., 3(3): 136-140. Kussman , J

Residues of organochlorine pesticides and polychlorinated biphenyls and autopsy data for bald eagles, 1971-72

USGS Publications Warehouse

Cromartie, E.; Reichel, W.L.; Locke, L.N.; Belisle, A.A.; Kaiser, T.E.; Lamont, T.G.; Mulhern, B.M.; Prouty, R.M.; Swineford, D.M.

1975-01-01

Thirty-seven bald eagles found sick or dead in 18 States during 1971-72 were analyzed for organochlorine pesticides and polychlorinated biphenyls (PCB's). DDE and PCB's were detected in all bald eagle carcasses; 30 carcasses contained DDD and 28 contained dieldrin. Four eagles contained possibly lethal levels of dieldrin and nine eagles had been poisoned by thallium. Autopsies revealed that illegal shooting was the most common cause of mortality. Since 1964 when data were first collected, 8 of the 17 eagles obtained from Maryland, Virginia, South Carolina, and Florida possibly died from dieldrin poisoning; all four specimens from Maryland and Virginia were from the Chesapeake Bay Tidewater area.

Mercury contamination in Idaho bald eagles, Haliaeetus leucocephalus.

PubMed

Bechard, Marc J; Perkins, Dusty N; Kaltenecker, Gregory S; Alsup, Steve

2009-11-01

Because mercury contamination is potentially threatening to bald eagle (Haliaeetus leucocephalus) populations, we collected molted feathers at nests to determine the level of contamination in bald eagles in the state of Idaho, USA. Eagle feathers contained measurable amounts of cadmium (Cd), chromium (Cr), selenium (Se), lead (Pb), as well as mercury (Hg). Cadmium, Cr, Se, and Pb levels averaged 0.17, 4.68, 2.02, and 1.29 mg/kg dry weight, respectively, and were at or below concentrations indicated as causing reproductive failure in bald eagles. Mercury contamination was found to be the highest averaging 18.74 mg/kg dry weight. Although a concentration of only 7.5 mg/kg dry weight Hg in bird feathers can cause reduced productivity and even sterility, all of the eagles we sampled bred successfully and the population of bald eagles continues to grow annually throughout the state.

Evidence of Bald Eagles feeding on freshwater mussels

Treesearch

Teryl G. Grubb; Michael A. Coffey

1982-01-01

A 1978 study of the winter habitat of the Bald Eagle (Haliaeetus leucocephalus) in the Coconino National Forest, Arizona, indicated repeated and potentially heavy use of a freshwater mussel (Anodonta corpulenta) in the eagles’ diet. As many as 10 eagles (five adults and five immatures) were observed at Upper Lake Mary near...

77 FR 43280 - Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Meeting With...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-24

... Storage Hydroelectric Project, Eagle Crest Energy; Notice of Meeting With the Bureau of Land Management a... Bureau of Land Management to improve agency coordination and discuss the agencies' overlapping jurisdictions (pursuant to the Federal Land Policy and Management Act and the Federal Power Act), on the Eagle...

78 FR 57444 - Eagle Fund III, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-18

... SMALL BUSINESS ADMINISTRATION [License No. 07/07-0116] Eagle Fund III, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle... Constitute Conflicts of Interest, of the Small Business Administration (``SBA'') Rules and Regulations. Eagle...

American Indian Self-Image Workshop Manual.

ERIC Educational Resources Information Center

Rainer, Howard T.

A self-image workshop manual for American Indians aims to help Indian people set goals and excel in whatever they plan in life. A section entitled "Are You an Eagle?" tells of the significance of eagles in traditional American Indian Culture, discusses those who merit an eagle feather for accomplishment, and lists characteristics of eagles (and…

Full-thickness skin wound healing using autologous keratinocytes and dermal fibroblasts with fibrin: bilayered versus single-layered substitute.

PubMed

Idrus, Ruszymah Bt Hj; Rameli, Mohd Adha bin P; Low, Kiat Cheong; Law, Jia Xian; Chua, Kien Hui; Latiff, Mazlyzam Bin Abdul; Saim, Aminuddin Bin

2014-04-01

Split-skin grafting (SSG) is the gold standard treatment for full-thickness skin defects. For certain patients, however, an extensive skin lesion resulted in inadequacies of the donor site. Tissue engineering offers an alternative approach by using a very small portion of an individual's skin to harvest cells for propagation and biomaterials to support the cells for implantation. The objective of this study was to determine the effectiveness of autologous bilayered tissue-engineered skin (BTES) and single-layer tissue-engineered skin composed of only keratinocytes (SLTES-K) or fibroblasts (SLTES-F) as alternatives for full-thickness wound healing in a sheep model. Full-thickness skin biopsies were harvested from adult sheep. Isolated fibroblasts were cultured using medium Ham's F12: Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum, whereas the keratinocytes were cultured using Define Keratinocytes Serum Free Medium. The BTES, SLTES-K, and SLTES-F were constructed using autologous fibrin as a biomaterial. Eight full-thickness wounds were created on the dorsum of the body of the sheep. On 4 wounds, polyvinyl chloride rings were used as chambers to prevent cell migration at the edge. The wounds were observed at days 7, 14, and 21. After 3 weeks of implantation, the sheep were euthanized and the skins were harvested. The excised tissues were fixed in formalin for histological examination via hematoxylin-eosin, Masson trichrome, and elastin van Gieson staining. The results showed that BTES, SLTES-K, and SLTES-F promote wound healing in nonchambered and chambered wounds, and BTES demonstrated the best healing potential. In conclusion, BTES proved to be an effective tissue-engineered construct that can promote the healing of full-thickness skin lesions. With the support of further clinical trials, this procedure could be an alternative to SSG for patients with partial- and full-thickness burns.

Behavior of a Spontaneously Arising Human Retinal Pigment Epithelial Cell Line Cultivated on Thin Alginate Film.

PubMed

Najafabadi, Hoda Shams; Soheili, Zahra-Soheila; Ganji, Shahla Mohammad

2015-01-01

A cell line spontaneously derived from human retinal pigment epithelium (hRPE) was cultured on alginate film gelatinized with different concentrations of neurobasal cell culture medium (NCCM) to assess its growth and morphological behavior on this naturally occurring polysaccharide. Neonatal human globes were used to isolate hRPE cells. They were cultured in Dulbecco's modified Eagle's-medium-and-Ham's-F12-medium-(DMEM/F12) supplemented with 10% fetal bovine serum (FBS). Cultures were continuously studied using phase contrast microscopy. After the nineth passage, cells were characterized through immunocytochemical analysis for Oct4, Chx10, and Pax6 and Ki67 markers. In each well of a 6-well microplate, 1 and 2% weight/volume (w/v) alginate in deionized water was added and gelatinized using 1× and 10× NCCM. hRPE cells were cultured at a density of 2 × 105 cells/well in alginate-coated microplates. After 5 days, hRPE colonies were harvested and re-plated on polystyrene substrates. Morphology and growth of hRPE cultures were determined during the next 2 weeks. The first few passages of the cultures were purely hRPE cells that revealed typical morphological features of the pigmented epithelium. They made spaces, devoid of cells, between hRPE cell monolayer and fill in the unoccupied spaces. They grew faster than native RPE cells and rapidly overgrew. Immunocytochemical test revealed that the founded cells expressed Chx10, Pax6, Ki67 and Oct4. The hRPE cells survived unlimitedly on alginate film and formed giant adjoining colonies. After re-plating, hRPE colonies adhered quickly on polystyrene and displayed native hRPE morphological features. Alginate film can support the survival and growth of hRPE cells and induce the cells to re-organize in tissue-like structures.

The mechanistic target of rapamycin (mTOR) pathway and S6 Kinase mediate diazoxide preconditioning in primary rat cortical neurons.

PubMed

Dutta, Somhrita; Rutkai, Ibolya; Katakam, Prasad V G; Busija, David W

2015-09-01

We examined the role of the mechanistic target of rapamycin (mTOR) pathway in delayed diazoxide (DZ)-induced preconditioning of cultured rat primary cortical neurons. Neurons were treated for 3 days with 500 μM DZ or feeding medium and then exposed to 3 h of continuous normoxia in Dulbecco's modified eagle medium with glucose or with 3 h of oxygen-glucose deprivation (OGD) followed by normoxia and feeding medium. The OGD decreased viability by 50%, depolarized mitochondria, and reduced mitochondrial respiration, whereas DZ treatment improved viability and mitochondrial respiration, and suppressed reactive oxygen species production, but did not restore mitochondrial membrane potential after OGD. Neuroprotection by DZ was associated with increased phosphorylation of protein kinase B (Akt), mTOR, and the major mTOR downstream substrate, S6 Kinase (S6K). The mTOR inhibitors rapamycin and Torin-1, as well as S6K-targeted siRNA abolished the protective effects of DZ. The effects of DZ on mitochondrial membrane potential and reactive oxygen species production were not affected by rapamycin. Preconditioning with DZ also changed mitochondrial and non-mitochondrial oxygen consumption rates. We conclude that in addition to reducing reactive oxygen species (ROS) production and mitochondrial membrane depolarization, DZ protects against OGD by activation of the Akt-mTOR-S6K pathway and by changes in mitochondrial respiration. Ischemic strokes have limited therapeutic options. Diazoxide (DZ) preconditioning can reduce neuronal damage. Using oxygen-glucose deprivation (OGD), we studied Akt/mTOR/S6K signaling and mitochondrial respiration in neuronal preconditioning. We found DZ protects neurons against OGD via the Akt/mTOR/S6K pathway and alters the mitochondrial and non-mitochondrial oxygen consumption rate. This suggests that the Akt/mTOR/S6k pathway and mitochondria are novel stroke targets. © 2015 International Society for Neurochemistry.

Biotelemetry data for golden eagles (Aquila chrysaetos) captured in coastal southern California, November 2014–February 2016

USGS Publications Warehouse

Tracey, Jeff A.; Madden, Melanie C.; Sebes, Jeremy B.; Bloom, Peter H.; Katzner, Todd E.; Fisher, Robert N.

2016-04-21

The status of golden eagles (Aquila chrysaetos) in coastal southern California is unclear. To address this knowledge gap, the U.S. Geological Survey (USGS) in collaboration with local, State, and other Federal agencies began a multi-year survey and tracking program of golden eagles to address questions regarding habitat use, movement behavior, nest occupancy, genetic population structure, and human impacts on eagles. Golden eagle trapping and tracking efforts began in October 2014 and continued until early March 2015. During the first trapping season that focused on San Diego County, we captured 13 golden eagles (8 females and 5 males). During the second trapping season that began in November 2015, we focused on trapping sites in San Diego, Orange, and western Riverside Counties. By February 23, 2016, we captured an additional 14 golden eagles (7 females and 7 males). In this report, biotelemetry data were collected between November 22, 2014, and February 23, 2016. The location data for eagles ranged as far north as San Luis Obispo, California, and as far south as La Paz, Baja California, Mexico.

Sub-basaltic Imaging of Ethiopian Mesozoic Sediments Using Surface Wave Dispersion

NASA Astrophysics Data System (ADS)

Mammo, T.; Maguire, P.; Denton, P.; Cornwell, D.

2003-12-01

The Ethiopia Afar Geoscientific Lithospheric Experiment (EAGLE) involved the deployment of a 400km NW-SE cross-rift profile across the Main Ethiopian Rift. The profile extended to about 150km on either side of the rift over the uplifted Ethiopian plateau characterized by voluminous Tertiary flood basalts covering a thick sequence of Mesozoic sediments. These consist of three major stratigraphic units, the Cretaceous Upper Sandstone (medium grained, friable and moderately to well-sorted) overlying the Jurassic Antalo limestone (with intercalations of marl, shale, mudstone and gypsum) above the Triassic Adigrat sandstone. These sediments are suggested to be approximately 1.5km thick at the north-western end of the profile, thickening to the south-east. They are considered a possible hydrocarbon reservoir and therefore crucial to the economy of Ethiopia. The EAGLE cross-rift profile included the deployment of 97 Guralp 6TD seismometers (30sec - 80Hz bandwidth) at a nominal 5km spacing. A 5.75 tonne explosion from the Muger quarry detonated specifically for the EAGLE project generated the surface waves used in this study. Preliminary processing involving the multiple filter technique has enabled the production of group velocity dispersion curves. These curves have been inverted to provide 1-D shear wave models, with the intention of providing an in-line cross-rift profile of Mesozoic sediment thickness. Preliminary results suggest that the sediments can be distinguished from both overlying plateau basalt and underlying basement, with their internal S-wave velocity structure possibly indicating that the three sediment units described above can be separately identified.

The Eagle Nebula: a spectral template for star forming regions

NASA Astrophysics Data System (ADS)

Flagey, Nicolas; Boulanger, Francois; Carey, Sean; Compiegne, Mathieu; Dwek, Eli; Habart, Emilie; Indebetouw, Remy; Montmerle, Thierry; Noriega-Crespo, Alberto

2008-03-01

IRAC and MIPS have revealed spectacular images of massive star forming regions in the Galaxy. These vivid illustrations of the interaction between the stars, through their winds and radiation, and their environment, made of gas and dust, still needs to be explained. The large scale picture of layered shells of gas components, is affected by the small scale interaction of stars with the clumpy medium that surrounds them. To understand spatial variations of physical conditions and dust properties on small scales, spectroscopic imaging observations are required on a nearby object. The iconic Eagle Nebula (M16) is one of the nearest and most observed star forming region of our Galaxy and as such, is a well suited template to obtain this missing data set. We thus propose a complete spectral map of the Eagle Nebula (M16) with the IRS/Long Low module (15-38 microns) and MIPS/SED mode (55-95 microns). Analysis of the dust emission, spectral features and continuum, and of the H2 and fine-structure gas lines within our models will provide us with constraints on the physical conditions (gas ionization state, pressure, radiation field) and dust properties (temperature, size distribution) at each position within the nebula. Only such a spatially and spectrally complete map will allow us to characterize small scale structure and dust evolution within the global context and understand the impact of small scale structure on the evolution of dusty star forming regions. This project takes advantage of the unique ability of IRS at obtaining sensitive spectral maps covering large areas.

Lead and mercury in fall migrant golden eagles from western North America.

PubMed

Langner, Heiko W; Domenech, Robert; Slabe, Vincent A; Sullivan, Sean P

2015-07-01

Lead exposure from ingestion of bullet fragments is a serious environmental hazard to eagles. We determined blood lead levels (BLL) in 178 golden eagles (Aquila chrysaetos) captured during fall migration along a major North American flyway. These eagles spent the breeding season distributed over a large range and are the best currently available representation of free flying golden eagles on the continent. We found 58 % of these eagles containing increased BLL > 0.1 mg/L; 10 % were clinically lead poisoned with BLL > 0.6 mg/L; and 4 % were lethally exposed with BLL > 1.2 mg/L. No statistical difference in BLL existed between golden and bald eagles (Haliaeetus leucocephalus). Golden eagles captured on carrion had higher BLL than those captured using live bait suggesting differences in feeding habits among individuals. Median BLL increased with age class. We propose a conceptual model for the long-term increase in BLL after ingestion of lead particles. The mean blood mercury level in golden eagles was 0.023 mg/L. We evaluate a field test for BLL that is based on anodic stripping voltammetry. This cost-effective and immediate method correlated well with results from inductively coupled plasma-mass spectrometry, although results needed to be corrected for each calibration of the test kit.

Movements and landscape use of Eastern Imperial Eagles Aquila heliaca in Central Asia

USGS Publications Warehouse

Poessel, Sharon; Bragin, Evgeny A.; Sharpe, Peter B.; Garcelon, David K.; Bartoszuk, Kordian; Katzner, Todd E.

2018-01-01

Capsule: We describe ecological factors associated with movements of a globally declining raptor species, the Eastern Imperial Eagle Aquila heliaca.Aims: To describe the movements, habitat associations and resource selection of Eastern Imperial Eagles marked in Central Asia.Methods: We used global positioning system (GPS) data sent via satellite telemetry devices deployed on Eastern Imperial Eagles captured in Kazakhstan to calculate distances travelled and to associate habitat and weather variables with eagle locations collected throughout the annual cycle. We also used resource selection models to evaluate habitat use of tracked birds during autumn migration. Separately, we used wing-tagging recovery data to broaden our understanding of wintering locations of eagles.Results: Eagles tagged in Kazakhstan wintered in most countries on the Arabian Peninsula, as well as Iran and India. The adult eagle we tracked travelled more efficiently than did the four pre-adults. During autumn migration, telemetered eagles used a mixture of vegetation types, but during winter and summer, they primarily used bare and sparsely vegetated areas. Finally, telemetered birds used orographic updrafts to subsidize their autumn migration flight, but they relied on thermal updrafts during spring migration.Conclusion: Our study is the first to use GPS telemetry to describe year-round movements and habitat associations of Eastern Imperial Eagles in Central Asia. Our findings provide insight into the ecology of this vulnerable raptor species that can contribute to conservation efforts on its behalf.

Wintering Golden Eagles on the coastal plain of South Carolina

DOE PAGES

Vukovich, Mark; Turner, Kelsey L.; Grazia, Tracy E.; ...

2015-10-01

Golden Eagles (Aquila chrysaetos) are rare winter residents in eastern North America, with most found along the Appalachian Mountains and few reported on the coastal plain of the Carolinas. We used remote cameras baited with wild pig (Sus scrofa) and white-tailed deer (Odocoileus virginianus) carcasses to detect, age, and individually identify Golden Eagles on the U.S. Department of Energy’s Savannah River Site on the coastal plain of South Carolina. We identified eight individual Golden Eagles during the winters of 2013–2014 and 2014–2015, with one detected during both winters. We detected eagles for 19 and 66 calendar days during the wintersmore » of 2013–2014 and 2014–2015, respectively, with two adult eagles detected for 30 and 31 calendar days in 2014–2015. Eagles typically scavenged on carcasses for a few days, left, and then returned when cameras were baited with another carcass, suggesting they had remained in the area. These observations suggest that large tracts of forests on the coastal plain may be important wintering areas for some Golden Eagles and, further, that other areas in the coastal plain of the southeastern United States may also harbor wintering eagles. Identification of wintering areas of Golden Eagles in the east will be an important step in the conservation of this protected species, and camera traps baited with carcasses can be an effective tool for such work.« less

Wintering Golden Eagles on the coastal plain of South Carolina

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vukovich, Mark; Turner, Kelsey L.; Grazia, Tracy E.

Golden Eagles (Aquila chrysaetos) are rare winter residents in eastern North America, with most found along the Appalachian Mountains and few reported on the coastal plain of the Carolinas. We used remote cameras baited with wild pig (Sus scrofa) and white-tailed deer (Odocoileus virginianus) carcasses to detect, age, and individually identify Golden Eagles on the U.S. Department of Energy’s Savannah River Site on the coastal plain of South Carolina. We identified eight individual Golden Eagles during the winters of 2013–2014 and 2014–2015, with one detected during both winters. We detected eagles for 19 and 66 calendar days during the wintersmore » of 2013–2014 and 2014–2015, respectively, with two adult eagles detected for 30 and 31 calendar days in 2014–2015. Eagles typically scavenged on carcasses for a few days, left, and then returned when cameras were baited with another carcass, suggesting they had remained in the area. These observations suggest that large tracts of forests on the coastal plain may be important wintering areas for some Golden Eagles and, further, that other areas in the coastal plain of the southeastern United States may also harbor wintering eagles. Identification of wintering areas of Golden Eagles in the east will be an important step in the conservation of this protected species, and camera traps baited with carcasses can be an effective tool for such work.« less

Interactive effects of prey and weather on golden eagle reproduction

USGS Publications Warehouse

Steenhof, Karen; Kochert, Michael N.; McDonald, T.L.

1997-01-01

1. The reproduction of the golden eagle Aquila chrysaetos was studied in southwestern Idaho for 23 years, and the relationship between eagle reproduction and jackrabbit Lepus californicus abundance, weather factors, and their interactions, was modelled using general linear models. Backward elimination procedures were used to arrive at parsimonious models.2. The number of golden eagle pairs occupying nesting territories each year showed a significant decline through time that was unrelated to either annual rabbit abundance or winter severity. However, eagle hatching dates were significantly related to both winter severity and jackrabbit abundance. Eagles hatched earlier when jackrabbits were abundant, and they hatched later after severe winters.3. Jackrabbit abundance influenced the proportion of pairs that laid eggs, the proportion of pairs that were successful, mean brood size at fledging, and the number of young fledged per pair. Weather interacted with prey to influence eagle reproductive rates.4. Both jackrabbit abundance and winter severity were important in predicting the percentage of eagle pairs that laid eggs. Percentage laying was related positively to jackrabbit abundance and inversely related to winter severity.5. The variables most useful in predicting percentage of laying pairs successful were rabbit abundance and the number of extremely hot days during brood-rearing. The number of hot days and rabbit abundance were also significant in a model predicting eagle brood size at fledging. Both success and brood size were positively related to jackrabbit abundance and inversely related to the frequency of hot days in spring.6. Eagle reproduction was limited by rabbit abundance during approximately twothirds of the years studied. Weather influenced how severely eagle reproduction declined in those years.7. This study demonstrates that prey and weather can interact to limit a large raptor population's productivity. Smaller raptors could be affected more strongly, especially in colder or wetter climates.

Groundwater Quality, Age, and Probability of Contamination, Eagle River Watershed Valley-Fill Aquifer, North-Central Colorado, 2006-2007

USGS Publications Warehouse

Rupert, Michael G.; Plummer, Niel

2009-01-01

The Eagle River watershed is located near the destination resort town of Vail, Colorado. The area has a fastgrowing permanent population, and the resort industry is rapidly expanding. A large percentage of the land undergoing development to support that growth overlies the Eagle River watershed valley-fill aquifer (ERWVFA), which likely has a high predisposition to groundwater contamination. As development continues, local organizations need tools to evaluate potential land-development effects on ground- and surface-water resources so that informed land-use and water management decisions can be made. To help develop these tools, the U.S. Geological Survey (USGS), in cooperation with Eagle County, the Eagle River Water and Sanitation District, the Town of Eagle, the Town of Gypsum, and the Upper Eagle Regional Water Authority, conducted a study in 2006-2007 of the groundwater quality, age, and probability of contamination in the ERWVFA, north-central Colorado. Ground- and surface-water quality samples were analyzed for major ions, nutrients, stable isotopes of hydrogen and oxygen in water, tritium, dissolved gases, chlorofluorocarbons (CFCs), and volatile organic compounds (VOCs) determined with very low-level laboratory methods. The major-ion data indicate that groundwaters in the ERWVFA can be classified into two major groups: groundwater that was recharged by infiltration of surface water, and groundwater that had less immediate recharge from surface water and had elevated sulfate concentrations. Sulfate concentrations exceeded the USEPA National Secondary Drinking Water Regulations (250 milligrams per liter) in many wells near Eagle, Gypsum, and Dotsero. The predominant source of sulfate to groundwater in the Eagle River watershed is the Eagle Valley Evaporite, which is a gypsum deposit of Pennsylvanian age located predominantly in the western one-half of Eagle County.

Optimal conditions for peripheral nerve storage in green tea polyphenol: an experimental study in animals.

PubMed

Matsumoto, Taiichi; Kakinoki, Ryosuke; Ikeguchi, Ryosuke; Hyon, Suong-Hyu; Nakamura, Takashi

2005-06-30

Our previous study demonstrated successful peripheral nerve storage for 1 month using polyphenol solution. We here report two studies to solve residual problems in using polyphenols as a storage solution for peripheral nerves. Study 1 was designed to determine the optimal concentration of the polyphenol solution and the optimal immersion period for nerve storage. Rat sciatic nerve segments were immersed in polyphenol solution at three different concentrations (2.5, 1.0, and 0.5 mg/ml) for three different periods (1, 7, and 26 days). Electrophysiological and morphological studies demonstrated that nerve regeneration from nerve segments that had been immersed in 1mg/ml polyphenol solution for 1 week and in Dulbecco's modified Eagle's medium (DMEM) for the subsequent 3 weeks was superior to the regeneration in other treatment groups. In study 2, the permeability of nerve tissue to polyphenol solution was investigated using canine sciatic nerve segments stored in 1.0mg/ml polyphenol solution for 1 week and in DMEM for the subsequent 3 weeks. Electron microscopy revealed that the Schwann cell structure within 500-700 microm of the perineurium was preserved, but cells deeper than 500-700 microm were badly damaged or had disappeared. The infiltration limit for polyphenol solution into neural tissue is inferred to be 500-700 microm.

Bovine lactotroph cultures for the study of prolactin synthesis functions.

PubMed

Wang, Jianfa; Yang, Zhanqing; Fu, Shoupeng; Liu, Bingrun; Wu, Dianjun; Wang, Wei; Sun, Dongbo; Wu, Rui; Liu, Juxiong

2016-03-01

The aim of this study was to establish a bovine anterior pituitary-derived lactotroph (BAPDL) line that expresses prolactin (PRL) in vitro to study the mechanisms of bovine PRL synthesis and secretion. Immunohistochemistry assay of PRL in the newborn calves' anterior pituitary glands showed that most lactotrophs were located within the superior border of the lateral wings of the anterior pituitary. Tissues of the superior border of the lateral wings of the anterior pituitary were dispersed and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The limiting dilution method was used to establish BAPDL from single cell clone. BAPDL cells constantly expressed mRNAs for PRL and pituitary-specific transcription factor 1 (Pit-1) gene and grew steadily and rapidly in the DMEM supplemented with 10% FBS. PRL immunoreactivity was present in BAPDL at passage 20. The concentration of bovine PRL in BAPDL at passage 20 culture supernatant was decreased to below 35% compared with that in BAPDL at passage 1. The effects of human epidermal growth factor (hEGF) and dopamine (DA) on the expression and secretion of PRL in BAPDL at passage 4 were also investigated. The results are consistent with those of previous studies. Thus, it can be used successfully for studying the mechanisms of stimuli regulating PRL synthesis and release.

Stability of dietary polyphenols under the cell culture conditions: avoiding erroneous conclusions.

PubMed

Xiao, Jianbo; Högger, Petra

2015-02-11

Most data of bioactivity from dietary polyphenols have been derived from in vitro cell culture experiments. In this context, little attention is paid to potential artifacts due to chemical instability of these natural antioxidants. An early degradation time ((C)T10) and half-degradation time ((C)T50) were defined to characterize the stability of 53 natural antioxidants incubated in Dulbecco's modified Eagle's medium (DMEM) at 37 °C. The degree of hydroxylation of flavones and flavonols significantly influenced the stability in order resorcinol-type > catechol-type > pyrogallol-type, with the pyrogallol-type being least stable. In contrast, any glycosylation of polyphenols obviously enhanced their stability. However, the glycosylation was less important compared to the substitution pattern of the nucleus rings. Methoxylation of flavonoids with more than three hydroxyl groups typically improved their stability as did the hydrogenation of the C2═C3 double bond of flavonoids to corresponding flavanoids. There was no significant correlation between the antioxidant potential of polyphenols and their stability. Notably, the polyphenols were clearly more stable in human plasma than in DMEM, which may be caused by polyphenol-protein interactions. It is strongly suggested to carry out stability tests in parallel with cell culture experiments for dietary antioxidants with catechol or pyrogallol structures and pyrogallol-type glycosides in order to avoid artifacts.

Pulsed electrodeposition for the synthesis of strontium-substituted calcium phosphate coatings with improved dissolution properties.

PubMed

Drevet, Richard; Benhayoune, Hicham

2013-10-01

Strontium-substituted calcium phosphate coatings are synthesized by pulsed electrodeposition on titanium alloy (Ti6Al4V) substrates. Experimental conditions of the process are optimized in order to obtain a coating with a 5% atomic substitution of calcium by strontium which corresponds to the best observations on the osteoblast cells activity and on the osteoclast cells proliferation. The physical and chemical characterizations of the obtained coating are carried out by scanning electron microscopy associated to energy dispersive X-ray spectroscopy (EDXS) for X-ray microanalysis and the structural characterization of the coating is carried out by X-ray diffraction. The in vitro dissolution/precipitation properties of the coated substrates are investigated by immersion into Dulbecco's Modified Eagle Medium (DMEM) from 1h to 14 days. The calcium, phosphorus and strontium concentrations variations in the biological liquid are assessed by Induced Coupled Plasma - Atomic Emission Spectroscopy for each immersion time. The results show that under specific experimental conditions, the electrodeposition process is suitable to synthesize strontium-substituted calcium phosphate coatings. Moreover, the addition of hydrogen peroxide (H2O2) into the electrolytic solution used in the process allows us to observe a control of the strontium release during the immersion of the prosthetic materials into DMEM. © 2013.

Bioactivity and mineralization of natural hydroxyapatite from cuttlefish bone and Bioglass® co-sintered bioceramics.

PubMed

Cozza, Natascia; Monte, Felipe; Bonani, Walter; Aswath, Pranesh; Motta, Antonella; Migliaresi, Claudio

2018-02-01

In this study, bioactive hydroxyapatite (HAP)-based bioceramics starting from cuttlefish bone powders have been prepared and characterized. In particular, fragmented cuttlefish bone was co-sintered with 30 wt% of Bioglass ® -45S5 to synthesize HAP-based powders with enhanced mechanical properties and bioactivity. Commercial synthetic HAP was treated following the same procedure and used as a reference. The structure and composition of the bioceramics formulations were characterized using Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. After the thermal treatment of cuttlefish bone powder added with 30 wt% Bioglass, new phases with compositions of sodium calcium phosphate [Na 3 Ca 6 (PO 4 ) 5 ], β-tricalcium phosphate [Ca 3 (PO 4 )] and amorphous silica were detected. In vitro cell culture studies were performed by evaluating proliferation, metabolic activity and differentiation of human osteoblast-like cells (MG63). Scaffolds made with cuttlefish bone powder exhibited increased apatite deposition, alkaline phosphatase activity and cell proliferation compared with commercial synthetic HAP. In addition, the ceramic compositions obtained after the combination with Bioglass ® further enhanced the metabolic activity of MG63 cell and promoted the formation of a well-developed apatite layer after 7 days of incubation in Dulbecco's modified Eagle's medium. Copyright © 2017 John Wiley & Sons, Ltd.

Apoptotic induction of skin cancer cell death by plant extracts.

PubMed

Thuncharoen, Walairat; Chulasiri, Malin; Nilwarangkoon, Sirinun; Nakamura, Yukio; Watanapokasin, Ramida

2013-01-01

The aim of the present study was to investigate the effects of plant extracts on cancer apoptotic induction. Human epidermoid carcinoma A431 cell line, obtained from the American Type Culture Collection (ATCC, Manassas, VA), was maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 degrees C, 5% carbon dioxide (CO2). Plant extract solutions were obtained from S & J international enterprises public company limited. These plant extracts include 50% hydroglycol extracts from Etlingera elatior (Jack) R.M.Smith (torch ginger; EE), Rosa damascene (damask rose; DR) and Rafflesia kerrii Meijer (bua phut; RM). The cell viability, time and dose dependency were determined by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. A431 cells were treated with the plant extracts and stained with Hoechst 33342 fluorescent staining dye. Cell viability was demonstrated by the inhibitory concentration 50% (IC50). The anti-proliferative effects were shown to be dependent on time and dose. Typical characteristics of apoptosis which are cell morphological changes and chromatin condensation were clearly observed. The plant extracts was shown to be effective for anti-proliferation and induction of apoptosis cell death in skin cancer cells. Therefore, mechanisms underlying the cell death and its potential use for treatment of skin cancer will be further studied.

Protective layer formation on magnesium in cell culture medium.

PubMed

Wagener, V; Virtanen, S

2016-06-01

In the past, different studies showed that hydroxyapatite (HA) or similar calcium phosphates can be precipitated on Mg during immersion in simulated body fluids. However, at the same time, in most cases a dark grey or black layer is built under the white HA crystals. This layer seems to consist as well of calcium phosphates. Until now, neither the morphology nor its influence on Mg corrosion have been investigated in detail. In this work commercially pure magnesium (cp) was immersed in cell culture medium for one, three and five days at room temperature and in the incubator (37 °C, 5% CO2). In addition, the influence of proteins on the formation of a corrosion layer was investigated by adding 20% of fetal calf serum (FCS) to the cell culture medium in the incubator. In order to analyze the formed layers, SEM images of cross sections, X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transformed Infrared Spectroscopy (FTIR) measurements were carried out. Characterization of the corrosion behavior was achieved by electrochemical impedance spectroscopy (EIS) and by potentio-dynamic polarization in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C. Surface analysis showed that all formed layers consist mainly of amorphous calcium phosphate compounds. For the immersion at room temperature the Ca/P ratio indicates the formation of HA, while in the incubator probably pre-stages to HA are formed. The different immersion conditions lead to a variation in layer thicknesses. However, electrochemical characterization shows that the layer thickness does not influence the corrosion resistance of magnesium. The main influencing factor for the corrosion behavior is the layer morphology. Thus, immersion at room temperature leads to the highest corrosion protection due to the formation of a compact outer layer. Layers formed in the incubator show much worse performances due to completely porous structures. The existence of proteins in DMEM seems to hinder the formation of a corrosion layer. However, protein adsorption leads to similar results as concerns corrosion protection as the formed calcium phosphate layer. Copyright © 2016 Elsevier B.V. All rights reserved.

Alternative Fuels Data Center: Golden Eagle Distributors Inc. to Convert

Science.gov Websites

several years. Golden Eagle will convert all fleet vehicles to CNG in their six branch operations Entire Fleet to CNG Golden Eagle Distributors Inc. to Convert Entire Fleet to CNG to someone by E-mail Share Alternative Fuels Data Center: Golden Eagle Distributors Inc. to Convert Entire Fleet

78 FR 25263 - Eagle Mountain Pumped Storage Hydroelectric Project; Eagle Crest Energy; Notice of Meeting With...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

... Storage Hydroelectric Project; Eagle Crest Energy; Notice of Meeting With the Bureau of Land Management a.... Purpose of the Meeting: Commission staff will meet with the staff of the Bureau of Land Management to... Land Policy and Management Act and the Federal Power Act), on the Eagle Mountain Pumped Storage...

78 FR 26358 - Eagle Mountain Pumped Storage Hydroelectric Project, Eagle Crest Energy; Notice of Meeting With...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-06

... Storage Hydroelectric Project, Eagle Crest Energy; Notice of Meeting With the Bureau of Land Management a... of the Meeting: Commission staff will meet with the staff of the Bureau of Land Management to improve... Policy and Management Act and the Federal Power Act), on the Eagle Mountain Pumped Storage Hydroelectric...

From the inside out: Eagle Rock School Producing a New Generation of CES Teachers

ERIC Educational Resources Information Center

Condon, Dan

2008-01-01

In this article, the author gives an overview of Eagle Rock School's Teaching Fellowship Program which he founded in collaboration with Public Allies, Inc. and under the auspices of Eagle Rock's Professional Development Center. Eagle Rock's Teaching Fellowship has two perspectives: (1) local; and (2) global. Locally, Fellows contribute skills,…

76 FR 34103 - In the Matter of Areva Enrichment Services, LLC (Eagle Rock Enrichment Facility); Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-10

.... 10-899-02-ML-BD01] In the Matter of Areva Enrichment Services, LLC (Eagle Rock Enrichment Facility... gas centrifuge uranium enrichment facility--denoted as the Eagle Rock Enrichment Facility (EREF)--in... Information for Contention Preparation; In the Matter of Areva Enrichment Services, LLC (Eagle Rock Enrichment...

Geologic map of the Vail West quadrangle, Eagle County, Colorado

USGS Publications Warehouse

Scott, Robert B.; Lidke, David J.; Grunwald, Daniel J.

2002-01-01

This new 1:24,000-scale geologic map of the Vail West 7.5' quadrangle, as part of the USGS Western Colorado I-70 Corridor Cooperative Geologic Mapping Project, provides new interpretations of the stratigraphy, structure, and geologic hazards in the area on the southwest flank of the Gore Range. Bedrock strata include Miocene tuffaceous sedimentary rocks, Mesozoic and upper Paleozoic sedimentary rocks, and undivided Early(?) Proterozoic metasedimentary and igneous rocks. Tuffaceous rocks are found in fault-tilted blocks. Only small outliers of the Dakota Sandstone, Morrison Formation, Entrada Sandstone, and Chinle Formation exist above the redbeds of the Permian-Pennsylvanian Maroon Formation and Pennsylvanian Minturn Formation, which were derived during erosion of the Ancestral Front Range east of the Gore fault zone. In the southwestern area of the map, the proximal Minturn facies change to distal Eagle Valley Formation and the Eagle Valley Evaporite basin facies. The Jacque Mountain Limestone Member, previously defined as the top of the Minturn Formation, cannot be traced to the facies change to the southwest. Abundant surficial deposits include Pinedale and Bull Lake Tills, periglacial deposits, earth-flow deposits, common diamicton deposits, common Quaternary landslide deposits, and an extensive, possibly late Pliocene landslide deposit. Landscaping has so extensively modified the land surface in the town of Vail that a modified land-surface unit was created to represent the surface unit. Laramide movement renewed activity along the Gore fault zone, producing a series of northwest-trending open anticlines and synclines in Paleozoic and Mesozoic strata, parallel to the trend of the fault zone. Tertiary down-to-the-northeast normal faults are evident and are parallel to similar faults in both the Gore Range and the Blue River valley to the northeast; presumably these are related to extensional deformation that occurred during formation of the northern end of the Rio Grande rift system in Colorado. In the southwestern part of the map area, a diapiric(?) exposure of the Eagle Valley Evaporite exists and chaotic faults and folds suggest extensive dissolution and collapse of overlying bedrock, indicating the presence of a geologic hazard. Quaternary landslides are common and indicate that landslide hazards are widespread in the area, particularly where old slide deposits are disturbed by construction. The late Pliocene(?) landslide that consists largely of a smectitic upper Morrison Formation matrix and boulders of Dakota Sandstone is readily reactivated. Debris flows are likely to invade low-standing areas within the towns of Vail and West Vail where tributaries of Gore Creek issue from the mountains on the north side of the valley.

Estimated ages of some large giant sequoias: General Sherman keeps getting younger

USGS Publications Warehouse

Stephenson, N.L.

2000-01-01

During 1975-77, 168 bald eagles (Haliaeetus leucocephalus) found moribund or dead in 29 states were analyzed for organochlorine pesticides and polychlorinated biphenyls (PCBs); 32 specimens from 13 states were analyzed for polybrominated biphenyls (PBBs). PCBs were present in 166 bald eagle carcasses and DDE was found in 165. TDE and dieldrin were identified in 137 samples, trans-nonachlor in 118, and oxychlordane in 90. Brains of five eagles contained possible lethal levels of dieldrin, and two eagles possibly died of endrin poisoning. Nine eagle livers, analyzed because of suspected lead poisoning, contained high levels of lead. Twenty percent of the eagles died from shooting, the most common cause of death; this cause of death, however, has declined.

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells.

PubMed

Healy, G M; Teleki, S; von Seefried, A; Walton, M J; Macmorine, H G

1971-01-01

An improved tissue culture basal medium, CMRL-1969, supplemented with serum, has been evaluated by measuring the growth responses of primary cultures of trypsin-dispersed monkey kidney cells (PMKC) and of an established culture of a human diploid cell strain (HDCS). Medium H597, an early modification of medium 199 which has been used successfully in the preparation of poliomyelitis vaccine for 15 years, was used for comparison. In addition, parallel testing was done with Basal Medium Eagle (BME) widely used for the growth of HDCS. The improvements in basal medium CMRL-1969 are attributed to changes in amino acid concentrations, in vitamin composition, and, in particular, to enhanced buffering capacity. The latter has been achieved by the use of free-base amino acids and by increasing the dibasic sodium phosphate. The new medium has already been used to advantage for the production of polioviruses in PMKC where equivalent titers were obtained from cultures initiated with 70% of the number of cells required with earlier media. The population-doubling time was reduced in this system. Also, with small inocula of HDCS, the time required to obtain maximum cell yield was shorter with CMRL-1969 than with BME. Both media were supplemented with 10% calf serum. Maximum cell yields after repeated subcultivation in the new basal medium were greatly increased and the stability of the strain, as shown by chromosomal analysis, was not affected. Basal medium CMRL-1969 can be prepared easily in liquid or powdered form.

Wintering bald eagle trends in northern Arizona, 1975-2000

Treesearch

Teryl G. Grubb

2003-01-01

Between 1975 and 2000, 4,525 sightings of wintering bald eagles (Haliaeetus leucocephalus) were recorded at Mormon Lake in northern Arizona. Numbers of wintering eagles fluctuated little in the 20 years from 1975 through 1994 (5.5 ± 3.0 mean sightings per day). However, during the winters of 1995 through 1997 local record highs of 59 to 118 eagles...

World Eagle, The Monthly Social Studies Resource: Data, Maps, Graphs. 1990-1991.

ERIC Educational Resources Information Center

World Eagle, 1991

1991-01-01

This document consists of the 10 issues of "World Eagle" issued during the 1990-1991 school year. World Eagle is a monthly social studies resource in which demographic and geographic information is presented in the forms of maps, graphs, charts, and text. Each issue of World Eagle has a section that focuses on a particular topic, along with other…

Macroinvertebrate and algal community sample collection methods and data collected at selected sites in the Eagle River watershed, Colorado, 2000-07

USGS Publications Warehouse

Zuellig, Robert E.; Bruce, James F.

2010-01-01

State and local agencies are concerned about the effects of increasing urban development and human population growth on water quality and the biological condition of regional streams in the Eagle River watershed. In response to these needs, the U.S. Geological Survey initiated a study in cooperation with the Colorado River Water Conservation District, Eagle County, Eagle River Water and Sanitation District, Upper Eagle Regional Water Authority, Colorado Department of Transportation, City of Aurora, Town of Eagle, Town of Gypsum, Town of Minturn, Town of Vail, Vail Resorts, Colorado Springs Utilities, Denver Water, and the U.S. Department of Agriculture Forest Service. As part of this study, previously collected macroinvertebrate and algal data from the Eagle River watershed were compiled. This report includes macroinvertebrate data collected by the U.S. Geological Survey and(or) the U.S. Department of Agriculture Forest Service from 73 sites from 2000 to 2007 and algal data collected from up to 26 sites between 2000 and 2001 in the Eagle River watershed. Additionally, a brief description of the sample collection methods and data processing procedures are presented.

Characterizing Golden Eagle risk to lead and anticoagulant rodenticide exposure: A review

USGS Publications Warehouse

Herring, Garth; Eagles-Smith, Collin A.; Buck, Jeremy A.

2017-01-01

Contaminant exposure is among the many threats to Golden Eagle (Aquila chrysaetos) populations throughout North America, particularly lead poisoning and anticoagulant rodenticides (AR). These threats may act in concert with others (e.g., lead poisoning and trauma associated with striking objects) to exacerbate risk. Golden Eagles are skilled hunters but also exploit scavenging opportunities, making them particularly susceptible to contaminant exposure from ingesting tissues of poisoned or shot animals. Lead poisoning has long been recognized as an important source of mortality for Golden Eagles throughout North America. More recently, ARs have been associated with both sublethal and lethal effects in raptor species worldwide. In this review, we examine the current state of knowledge for lead and AR exposure in Golden Eagles, drawing from the broader raptor contaminant ecology literature. We examine lead and AR sources within Golden Eagle habitats, exposure routes and toxicity, effects on individuals and populations, synergistic effects, and data and information needs. Continued research addressing data needs and information gaps will help with Golden Eagle conservation planning.

Integrated Ground-based Hyperspectral Imaging and Geochemical Study of the Eagle Ford Group in West Texas

NASA Astrophysics Data System (ADS)

Sun, L.; Khan, S.; Godet, A.

2017-12-01

This study used ground-based hyperspectral imaging to map an outcrop of the Eagle Ford Group in west Texas. The Eagle Ford Group consists of alternating layers of mudstone - wackestone, grainstone - packstone facies and volcanic ash deposits with high total organic carbon content deposited during the Late Cenomanian - Turonian time period. It is one of the few unconventional source rock and reservoirs that have surface representations. Ground-based hyperspectral imaging scanned an outcrop and hand samples at close ranges with very fine spatial resolution (centimeter to sub-millimeter). Spectral absorption modeling of clay minerals and calcite with the modified Gaussian model (MGM) allowed quantification of variations of mineral abundances. Petrographic analysis confirmed mineral identifications and shed light on sedimentary textures. Major element geochemistry confirmed the mineral quantification. Enrichment of molybdenum (Mo) and uranium (U) indicated "unrestricted marine" paleo-hydrogeology and anoxic to euxinic paleo-redox bottom water conditions. Mineral quantification resulted in mapping of mudstone - wackestone, grainstone - packstone facies and claystones (volcanic ash beds). The lack of spatial associations between the grainstones and claystones on the outcrop calls into question the hypothesis that the primary productivity is controlled by iron availability from volcanic ash beds. Hyperspectral remote sensing data also helped in creating a virtual outcrop model with detailed mineralogical compositions, and provided reservoir analog to extract compositional and geo-mechanical characteristics and variations. The utilization of these new techniques in geo-statistical analysis provides a workflow for employing remote sensing in resource exploration and exploitation.

Integrated ground-based hyperspectral imaging and geochemical study of the Eagle Ford Group in West Texas

NASA Astrophysics Data System (ADS)

Sun, Lei; Khan, Shuhab; Godet, Alexis

2018-01-01

This study used ground-based hyperspectral imaging to map an outcrop of the Eagle Ford Group in west Texas. The Eagle Ford Group consists of alternating layers of mudstone - wackestone, grainstone - packstone facies and volcanic ash deposits with high total organic content deposited during the Cenomanian - Turonian time period. It is one of the few unconventional source rock and reservoirs that have surface representations. Ground-based hyperspectral imaging scanned an outcrop and hand samples at close ranges with very fine spatial resolution (centimeter to sub-millimeter). Spectral absorption modeling of clay minerals and calcite with the modified Gaussian model (MGM) allowed quantification of variations of mineral abundances. Petrographic analysis confirmed mineral identifications and shed light on sedimentary textures, and major element geochemistry supported the mineral quantification. Mineral quantification resulted in mapping of mudstone - wackestone, grainstone - packstone facies and bentonites (volcanic ash beds). The lack of spatial associations between the grainstones and bentonites on the outcrop calls into question the hypothesis that the primary productivity is controlled by iron availability from volcanic ash beds. Enrichment of molybdenum (Mo) and uranium (U) indicated "unrestricted marine" paleo-hydrogeology and anoxic to euxinic paleo-redox bottom water conditions. Hyperspectral remote sensing data also helped in creating a virtual outcrop model with detailed mineralogical compositions, and provided reservoir analog to extract compositional and geo-mechanical characteristics and variations. The utilization of these new techniques in geo-statistical analysis provides a workflow for employing remote sensing in resource exploration and exploitation.

Cold physical plasma treated buffered saline solution as effective agent against pancreatic cancer cells.

PubMed

Bekeschus, Sander; Kading, Andre; Schroder, Tim; Wende, Kristian; Hackbarth, Christine; Liedtke, Kim Rouven; van der Linde, Julia; von Woedtke, Thomas; Heidecke, Claus-Dieter; Partecke, Lars-Ivo

2018-05-07

Cold physical plasma has been suggested as a new anticancer tool recently. However, direct use of plasma is limited to visible tumors and in some clinical situations not feasible. This includes repetitive treatment of peritoneal metastases which commonly occur in advanced gastrointestinal cancer and in pancreatic cancer in particular. In case of diffuse intraperitoneal metastatic spread Hyperthermic Intraperitoneal Intraoperative Chemotherapy (HIPEC) is used as therapeutic approach. Plasma treated solutions may combine their suspected systemic non-toxic characteristics with the anticancer effects of HIPEC. Previous work has provided evidence for an anti-cancer efficacy of plasma treated cell culture medium but the clinical relevance of such an approach is low due to its complex formulation and lack of medical accreditation. Therefore, plasma treated phosphate-buffered saline (PBS) which closely resembles medically certified solutions was investigated for its cytotoxic effect on 2D monolayer murine pancreatic cancer cells in vitro. It significantly decreased cancer cell metabolisms and proliferation whereas plasma treated Dulbecco's Modified Eagle Medium had no effect. Moreover, tumor cell growth attenuation was significantly higher when compared to syngeneic primary murine fibroblasts. Both results were confirmed in a human pancreatic cancer cell line. Finally, plasma treated PBS also decreased tumor sizes of pancreatic tumors in the TUM-CAM model in a three-dimensional manner, and induction of apoptosis was found to be responsible for all anticancer effects identified. Altogether, plasma treated PBS inhibited cell growth in 2D and 3D models of cancer. These results may help facilitating the development of new plasma derived anticancer agent with clinical relevance in the future. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study.

PubMed

Pillai, Mamatha M; Elakkiya, V; Gopinathan, J; Sabarinath, C; Shanthakumari, S; Sahanand, K Santosh; Dinakar Rai, B K; Bhattacharyya, Amitava; Selvakumar, R

2016-10-01

The present study investigates the impact of biomolecules (biotin, glucose, chondroitin sulphate, proline) as supplement, (individual and in combination) on primary human meniscus cell proliferation. Primary human meniscus cells isolated from patients undergoing meniscectomy were maintained in Dulbecco's Modified Eagle's Medium (DMEM). The isolated cells were treated with above mentioned biomolecules as individual (0-100 µg/ml) and in combinations, as a supplement to DMEM. Based on the individual biomolecule study, a unique combination of biomolecules (UCM) was finalized using one way ANOVA analysis. With the addition of UCM as supplement to DMEM, meniscal cells reached 100 % confluency within 4 days in 60 mm culture plate; whereas the cells in medium devoid of UCM, required 36 days for reaching confluency. The impact of UCM on cell viability, doubling time, histology, gene expression, biomarkers expression, extra cellular matrix synthesis, meniscus cell proliferation with respect to passages and donor's age were investigated. The gene expression studies for E-cadherin and peroxisome proliferator-activated receptor (PPAR∆) using RT-qPCR and immunohistochemical analysis for Ki67, CD34 and Vimentin confirmed that UCM has significant impact on cell proliferation. The extracellular collagen and glycosaminoglycan secretion in cells supplemented with UCM were found to increase by 31 and 37 fold respectively, when compared to control on the 4th day. The cell doubling time was reduced significantly when supplemented with UCM. The addition of UCM showed positive influence on different passages and age groups. Hence, this optimized UCM can be used as an effective supplement for meniscal tissue engineering.

Platelet lysate 3D scaffold supports mesenchymal stem cell chondrogenesis: an improved approach in cartilage tissue engineering.

PubMed

Moroz, Andrei; Bittencourt, Renata Aparecida Camargo; Almeida, Renan Padron; Felisbino, Sérgio Luis; Deffune, Elenice

2013-01-01

Articular lesions are still a major challenge in orthopedics because of cartilage's poor healing properties. A major improvement in therapeutics was the development of autologous chondrocytes implantation (ACI), a biotechnology-derived technique that delivers healthy autologous chondrocytes after in vitro expansion. To obtain cartilage-like tissue, 3D scaffolds are essential to maintain chondrocyte differentiated status. Currently, bioactive 3D scaffolds are promising as they can deliver growth factors, cytokines, and hormones to the cells, giving them a boost to attach, proliferate, induce protein synthesis, and differentiate. Using mesenchymal stem cells (MSCs) differentiated into chondrocytes, one can avoid cartilage harvesting. Thus, we investigated the potential use of a platelet-lysate-based 3D bioactive scaffold to support chondrogenic differentiation and maintenance of MSCs. The MSCs from adult rabbit bone marrow (n = 5) were cultivated and characterized using three antibodies by flow cytometry. MSCs (1 × 10(5)) were than encapsulated inside 60 µl of a rabbit platelet-lysate clot scaffold and maintained in Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 supplemented with chondrogenic inductors. After 21 days, the MSCs-seeded scaffolds were processed for histological analysis and stained with toluidine blue. This scaffold was able to maintain round-shaped cells, typical chondrocyte metachromatic extracellular matrix deposition, and isogenous group formation. Cells accumulated inside lacunae and cytoplasm lipid droplets were other observed typical chondrocyte features. In conclusion, the usage of a platelet-lysate bioactive scaffold, associated with a suitable chondrogenic culture medium, supports MSCs chondrogenesis. As such, it offers an alternative tool for cartilage engineering research and ACI.

The effect of nicotine and cotinine on human gingival fibroblasts attachment to root surfaces.

PubMed

Esfahrood, Zeinab Rezaei; Zamanian, Amirhosein; Torshabi, Maryam; Abrishami, Maryam

2015-09-01

Different compounds of smoking (e.g., nicotine and cotinine) are risk factors for various diseases such as oral cancer and periodontal diseases. Some studies reported the negative effects of nicotine on cell proliferation and differentiation. The present in vitro study assessed the effects of nicotine and cotinine (long-acting metabolite of nicotine) on the attachment and viability of human gingival fibroblast (HGF) cells to tooth root surfaces. A total of 70 teeth specimens were placed into 48-well culture plates and covered with HGF cell suspension, in complete Dulbecco's modified Eagle's medium culture medium containing 1 nM, 1 μm, 1 mM, and 5 mM of nicotine and cotinine concentrations. Cellular attachment and viability measured using an MTT assay and a scanning electron microscope were used for cell morphological evaluation. After 24 h, low (nanomolar and micromolar) and high concentrations (millimolar) of nicotine and cotinine caused a significant reduction in the initial cell adhesion in comparison with the control group, but no significant difference was observed between the nicotine and the cotinine groups (p<0.05). Dentally attached cells with low concentrations of nicotine and cotinine proliferated 48 h after exposure, the same as the control group. However, dentally attached cells with high concentrations of nicotine and cotinine (especially 5 mM) did not proliferate 24 h after exposure (p<0.05). Low concentrations of nicotine and cotinine caused a reduction in the initial cell adhesion. However, no significant adverse effects on the proliferation of attached cells were seen in the longer period. High concentrations of nicotine and cotinine have adverse effects on the cell adhesion and proliferation of HGF cells.

Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response.

PubMed

Maiorano, Gabriele; Sabella, Stefania; Sorce, Barbara; Brunetti, Virgilio; Malvindi, Maria Ada; Cingolani, Roberto; Pompa, Pier Paolo

2010-12-28

The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV-visible, plasmon resonance light scattering), that proteins-NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle's medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the two media, in the presence of 15 nm AuNPs. We observed that proteins/NP complexes formed in RPMI are more abundantly internalized in cells as compared to DMEM, overall exerting higher cytotoxic effects. These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests.

Nitrate salts suppress sporulation and production of enterotoxin in Clostridium perfringens strain NCTC8239.

PubMed

Yasugi, Mayo; Otsuka, Keisuke; Miyake, Masami

2016-10-01

Clostridium perfringens type A is a common source of food-borne illness in humans. Ingested vegetative cells sporulate in the small intestinal tract and in the process produce C. perfringens enterotoxin (CPE). Although sporulation plays a critical role in the pathogenesis of food-borne illness, the molecules triggering/inhibiting sporulation are still largely unknown. It has previously been reported by our group that sporulation is induced in C. perfringens strain NCTC8239 co-cultured with Caco-2 cells in Dulbecco's Modified Eagle Medium (DMEM). In contrast, an equivalent amount of spores was not observed when bacteria were co-cultured in Roswell Park Memorial Institute-1640 medium (RPMI). In the present study it was found that, when these two media are mixed, RPMI inhibits sporulation and CPE production induced in DMEM. When a component of RPMI was added to DMEM, it was found that calcium nitrate (Ca[NO 3 ] 2 ) significantly inhibits sporulation and CPE production. The number of spores increased when Ca(NO 3 ) 2 -deficient RPMI was used. The other nitrate salts significantly suppressed sporulation, whereas the calcium salts used did not. qPCR revealed that nitrate salts increased expression of bacterial nitrate/nitrite reductase. Furthermore, it was found that nitrite and nitric oxide suppress sporulation. In the sporulation stages, Ca(NO 3 ) 2 down-regulated the genes controlled by Spo0A, a master regulator of sporulation, but not spo0A itself. Collectively, these results indicate that nitrate salts suppress sporulation and CPE production by down-regulating Spo0A-regulated genes in C. perfringens strain NCTC8239. Nitrate reduction may be associated with inhibition of sporulation. © 2016 The Societies and John Wiley & Sons Australia, Ltd.

Cryopreservation of amniotic membrane with and without glycerol additive.

PubMed

Wagner, Malina; Walter, Peter; Salla, Sabine; Johnen, Sandra; Plange, Niklas; Rütten, Stephan; Goecke, Tamme W; Fuest, Matthias

2018-06-01

Amniotic membrane (AM) is an essential tool in ocular surface reconstruction. In this study, we analyzed the differential effects of glycerol and straight storage at - 80 °C for up to 6 months on the structural, biological, and mechanical properties of amniotic membrane (AM). Human placentae of 11 different subjects were analyzed. AMs were stored at - 80 °C, either with a 1:1 mixture of Dulbecco's modified Eagle medium and glycerol (glycerol) or without any medium or additives (straight). Histological image analysis, tensile strength, cell viability, and basic fibroblast growth factor (bFGF) secretion were evaluated at 0.5, 1, 3, and 6 months. Histologically, neither glycerol nor straight storage significantly altered the epithelial or stromal structure of the AM. However, the cell number of the stroma was significantly reduced during the freezing process, independently of the storage method (p = 0.05-0.001). Tensile strength and Young's modulus were not influenced by the storage method, but longer storage periods significantly increased the tensile strength of the AMs (p = 0.028). Cell viability was higher in glycerol rather than straight AM samples for up to 3 months of storage (p = 0.047-0.03). Secretion of bFGF at 3 months of storage was significantly higher in glycerol versus straight frozen AM samples (p = 0.04). Glycerol led to higher cell viability and higher bFGF secretion for up to 3 months of AM storage. However, no significant differences between the two methods were observed at 6 months of storage at - 80 °C.

Effects of TiO2 nanoparticles on the NO2 - levels in cell culture media analysed by Griess colorimetric methods

NASA Astrophysics Data System (ADS)

Popescu, Traian; Lupu, Andreea R.; Diamandescu, Lucian; Tarabasanu-Mihaila, Doina; Teodorescu, Valentin S.; Raditoiu, Valentin; Purcar, Violeta; Vlaicu, Aurel M.

2013-02-01

The Griess assay has been used to determine the possible changes in the measured NO2 - concentrations induced by TiO2 nanoparticles in three types of nitrite-containing samples: aqueous NaNO2 solutions with known concentrations, and two types of cell culture media—Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco's Modified Eagle Medium (DMEM-F12) used either as delivered or enriched in NO2 - by NaNO2 addition. We have used three types of titania with average particle sizes between 10 and 30 nm: Degussa P25 and two other samples (undoped and Fe3+-doped anatase TiO2) synthesised by a hydrothermal route in our laboratory. The structural, morphological, optical and physicochemical characteristics of the used materials have been studied by X-ray diffraction, transmission electron microscopy (EDX), Mössbauer spectroscopy, Brunauer-Emmett-Teller nitrogen adsorption, UV-Vis reflectance spectroscopy, dynamic light scattering and diffuse reflectance infrared Fourier transform spectroscopy. The opacity and sedimentation behaviour of the studied TiO2 suspensions have been investigated by photometric attenuance measurements at 540 nm. To account for the photocatalytic properties of titania in a biologically relevant context, multiple Griess tests have been performed under controlled exposure to laboratory natural daylight illumination. The results show significant variations of light attenuance (associated with NO2 - concentrations in the Griess test) depending on the opacity, sedimentation behaviour, NO2 - adsorption and photocatalytic properties of the tested TiO2 nanomaterials. These findings identify material characteristics recommended to be considered when analysing the results of Griess tests performed in biological studies involving TiO2 nanoparticles.

Tumor Necrosis Factor-α and Interleukin (IL)-1β, IL-6, and IL-8 Impair In Vitro Migration and Induce Apoptosis of Gingival Fibroblasts and Epithelial Cells, Delaying Wound Healing.

PubMed

Basso, Fernanda G; Pansani, Taisa N; Turrioni, Ana Paula S; Soares, Diana G; de Souza Costa, Carlos Alberto; Hebling, Josimeri

2016-08-01

Multiple factors affect oral mucosal healing, such as the persistence of an inflammatory reaction. The present study evaluates effects of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, IL-6, and IL-8 on epithelial cells (ECs) and human gingival fibroblasts (GFs) in vitro. GFs and ECs were seeded in 96-well plates (1 × 10(4) cells/well) in plain culture medium (Dulbecco's modified Eagle's medium [DMEM]) containing 1% antibiotic/antimycotic solution and 10% fetal bovine serum, and incubated for 24 hours. Both cell lines were exposed for 24 hours to the following cytokines: 1) TNF-α (100 ng/mL); 2) IL-1β (1 ng/mL); 3) IL-6 (10 ng/mL); and 4) IL-8 (10 ng/mL). All cytokines were diluted in serum-free DMEM. Control cultures were exposed only to serum-free DMEM. Effects of exposure to inflammatory cytokines were determined by means of: 1) apoptosis (anexin V); 2) cell migration (wound healing assay); 3) inflammatory cytokine synthesis (enzyme-linked immunosorbent assay). Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests (α = 0.05). Increased apoptosis rates were noted when cells were exposed to inflammatory cytokines, except ECs exposed to IL-1β. Cell migration was negatively affected by all inflammatory cytokines for both cell lines. ECs and GFs exposed to IL-6 and IL-8 significantly increased synthesis of TNF-α and IL-1β. Demonstrated results indicate negative effects of tested inflammatory cytokines on ECs and GFs, inducing apoptosis and impairing cell migration. These results can justify delayed oral mucosa healing in the presence of inflammatory reaction.

Advanced glycation end products of bovine serum albumin-induced endothelial-to-mesenchymal transition in cultured human and monkey endothelial cells via protein kinase B signaling cascades.

PubMed

Ma, Jianli; Liu, Ting; Dong, Xiaoguang

2010-12-09

Advanced glycation end products of BSA (AGE-BSA) participate in the pathogenesis of diabetic vascular disease. However, the role of AGE-BSA in diabetic retinopathy, especially in retinal neovascularization, remains incomplete. This study aimed to determine the contributions of AGE-BSA in the endothelial-to-mesenchymal transition (EnMT) of cultured human and monkey endothelial cell lines and the mechanism that may be related with the transition. Monkey choroid-retinal endothelial cells (RF/6A) and human umbilical vein endothelial cells (HUVEC) were cultured in Dulbecco's modified Eagle's Medium (DMEM) and Ham's F12 medium containing 200 mg/l AGE-BSA. The expression of VE-cadherin, β-catenin, vimentin, N-cadherin, and protein kinase B (AKT2) was observed by immunocytochemistry and flow cytometry. Cell motility was determined by migration assays; the endothelial function of the formatting tube was measured by tube formation assays, while the change in the polarity was measured using resistance instruments. The characteristics of EnMT included loss of endothelial markers of VE-cadherin and β-catenin, which were replaced by mesenchymal markers of vimentin and N-cadherin, enhanced migration and tube formation, and diminished polarity. AGE-BSA contributed to upregulation of the protein expression of VE-cadherin and β-catenin and downregulation of protein expression of vimentin and N-cadherin, leading to enhanced migration and tube formation and diminished polarity. During this process, expression of AKT2 was upregulated. AGE-BSA can induce EnMT of cultured human and monkey endothelial cells. The signal pathway involving AKT2 may play a role in this process.

Bald Eagles at Bay

ERIC Educational Resources Information Center

Laycock, George

1974-01-01

Describes the process of transplanting eggs from one nest to another in an attempt to aid in the strengthening of the eagle population. Discusses pressures exerted on eagles by hunting, trapping and pesticides. (SLH)

Modified PEHPS medium as an alternative for the in vitro culture of Giardia lamblia.

PubMed

Vargas-Villarreal, Javier; Mata-Cárdenas, Benito D; Hernández-García, Magda E; Garza-González, Jesús N; De La Garza-Salinas, Laura H; González-Salazar, Francisco

2014-01-01

Commercial culture media present interlot variations in biological activity. We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The objective of this work was to modify PEHPS medium to support the in vitro growth of Giardia lamblia. Inocula of 5 × 10(3) trophozoites/mL of G. lamblia were incubated at 36.5°C in modified PEHPS or TYI-S-33 medium. Then, the growths of the three Giardia strains in both media were compared. The logarithmic growth phase lasted 72 h; the mean yield of the strains ranged from 10.06 to 11.43 × 10(5) Giardia trophozoites/mL, and the range of duplication time in the three strains was from 5.67 to 6.06 in modified PEHPS medium. These growth characteristics were not significantly different from those obtained with TYI-S-33 medium. We conclude that modified PEHPS medium might be used for the axenic cultivation of G. lamblia.

Golden Eagle Monitoring Plan for the Desert Renewable Energy Conservation Plan

USGS Publications Warehouse

Wiens, David; Kolar, Patrick; Katzner, Todd

2018-01-01

This report describes options for monitoring the status and population trends of the golden eagle (Aquila chrysaetos) within the Desert Renewable Energy Conservation Plan (DRECP) area of Southern California in maintaining stable or increasing population in the planning area. The report profiles the ecology of golden eagles in the region and provides a range of potential sampling options to address monitoring needs and objectives. This approach also focused on links between changes in human land-use, golden eagle nesting and foraging habitat conditions, and population dynamics. The report outlines how monitoring data from demographic, prey, and habitat studies were used to develop a predictive demographic model for golden eagles in the DRECP area. Results from the model simulations suggest increases in renewable energy development could have negative consequences for population trajectories. Results also suggest site-specific conservation actions could reduce the magnitude of negative impacts to the local population of eagles. A monitoring framework is proposed including: (1) annual assessments of site-occupancy and reproduction by territorial pairs of golden eagles (including rates at which sites become colonized or vacated over time); (2) estimates of survival, movements, and intensity of use of landscapes by breeding and non-breeding golden eagles; (3) periodic (conducted every two to four years) assessments of nesting and foraging habitats, prey populations, and associations with land-use and management activities; and (4) updating the predictive demographic model with new information obtained on eagles and associated population stressors. The results of this research were published in the Journal of Rapture Research, Wiens, David,Inman, Rich D., Esque, Todd C., Longshore, Kathleen M. and Nussear, Kenneth (2017). Spatial Demographic Models to Inform Conservation Planning of Golden Eagles in Renewable Energy Landscapes. 51(3):234-257.

Does the order of invasive species removal matter? The case of the eagle and the pig.

PubMed

Collins, Paul W; Latta, Brian C; Roemer, Gary W

2009-09-14

Invasive species are recognized as a primary driver of native species endangerment and their removal is often a key component of a conservation strategy. Removing invasive species is not always a straightforward task, however, especially when they interact with other species in complex ways to negatively influence native species. Because unintended consequences may arise if all invasive species cannot be removed simultaneously, the order of their removal is of paramount importance to ecological restoration. In the mid-1990s, three subspecies of the island fox Urocyon littoralis were driven to near extinction on the northern California Channel Islands owing to heightened predation by golden eagles Aquila chrysaetos. Eagles were lured to the islands by an abundant supply of feral pigs Sus scrofa and through the process of apparent competition pigs indirectly facilitated the decline in foxes. As a consequence, both pigs and eagles had to be removed to recover the critically endangered fox. Complete removal of pigs was problematic: removing pigs first could force eagles to concentrate on the remaining foxes, increasing their probability of extinction. Removing eagles first was difficult: eagles are not easily captured and lethal removal was politically distasteful. Using prey remains collected from eagle nests both before and after the eradication of pigs, we show that one pair of eagles that eluded capture did indeed focus more on foxes. These results support the premise that if the threat of eagle predation had not been mitigated prior to pig removal, fox extinction would have been a more likely outcome. If complete eradication of all interacting invasive species is not possible, the order in which they are removed requires careful consideration. If overlooked, unexpected consequences may result that could impede restoration.

A pilot golden eagle population study in the Altamont Pass Wind Resource Area, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunt, G.

1995-05-01

Orloff and Flannery (1992) estimated that several hundred reports are annually killed by turbine collisions, wire strikes, and electrocutions at the Altamont Pass Wind Resource Area (WRA). The most common fatalities were those of red-tailed hawks (Buteo jamaicensis), American kestrels (Falco sparvatius), and golden eagles (Aquila chrysaetos), with lesser numbers of turkey vultures (Cathartes aura), common ravens (Corvus corax), bam owls (Tyto alba), and others. Among the species of raptors killed at Altamont Pass, the one whose local population is most likely to be impacted is the golden eagle. Besides its being less abundant than the others, the breeding andmore » recruitment rates of golden eagles are naturally slow, increasing their susceptibility to decline as a result of mortality influences. The golden eagle is a species afforded special federal protection because of its inclusion within the Bald Eagle Protection Act as amended in 1963. There are no provisions within the Act which would allow the killing ``taking`` of golden eagles by WRA structures. This report details the results of field studies conducted during 19941. The primary purpose of the investigation is to lay the groundwork for determining whether or not turbine strikes and other hazards related to energy at Altamont Pass may be expected to affect golden eagles on a population basis. We also seek an understanding of the physical and biotic circumstances which attract golden eagles to the WRA within the context of the surrounding landscape and the conditions under which they are killed by wind turbines. Such knowledge may suggest turbine-related or habitat modifications that would result in a lower incidence of eagle mortality.« less

Spatial structure in the diet of imperial eagles Aquila heliaca in Kazakhstan

USGS Publications Warehouse

Katzner, T.E.; Bragin, E.A.; Knick, S.T.; Smith, A.T.

2006-01-01

We evaluated the relationship between spatial variability in prey and food habits of eastern imperial eagles Aquila heliaca at a 90,000 ha national nature reserve in north-central Kazakhstan. Eagle diet varied greatly within the population and the spatial structure of eagle diet within the population varied according to the scale of measurement. Patterns in dietary response were inconsistent with expectations if either ontogenetic imprinting or competition determined diet choice, but they met expectations if functional response determined diet. Eagles nesting near a high-density prey resource used that resource almost exclusively. In contrast, in locations with no single high-density prey species, eagles' diet was more diverse. Our results demonstrate that spatial structuring of diet of vertebrate predators can provide important insight into the mechanisms that drive dietary decisions. ?? OIKOS.

Golden eagle (Aquila chrysaetos) habitat selection as a function of land use and terrain, San Diego County, California

USGS Publications Warehouse

Tracey, Jeff A.; Madden, Melanie C.; Bloom, Peter H.; Katzner, Todd E.; Fisher, Robert N.

2018-04-16

Beginning in 2014, the U.S. Geological Survey, in collaboration with Bloom Biological, Inc., began telemetry research on golden eagles (Aquila chrysaetos) captured in the San Diego, Orange, and western Riverside Counties of southern California. This work was supported by the San Diego Association of Governments, California Department of Fish and Wildlife, the U.S. Fish and Wildlife Service, the Bureau of Land Management, and the U.S. Geological Survey. Since 2014, we have tracked more than 40 eagles, although this report focuses only on San Diego County eagles.An important objective of this research is to develop habitat selection models for golden eagles. Here we provide predictions of population-level habitat selection for golden eagles in San Diego County based on environmental covariates related to land use and terrain.

Kleptoparasitism by bald eagles wintering in south-central Nebraska

USGS Publications Warehouse

Jorde, Dennis G.; Lingle, G.R.

1988-01-01

Kleptoparasitism on other raptors was one means by which Bald Eagles (Haliaeetus leucocephalus) secured food along the North Platte and Platte rivers during the winters of 1978-1980. Species kelptoparasitized were Ferruginous Hawk (Buteo regalis), Red-tailed Hawk (B. jamaicensis), Rough-legged Hawk (B. lagopus), Golden Eagle (Aquila chrysaetos), and Bald Eagle. Stealing of prey occurred more often during the severe winter of 1978-1979 when ice cover restricted eagles from feeding on fish than during the milder winter of 1979-1980. Kleptoparasitism occurred principally in agricultural habitats where large numbers of Mallards (Anas platyrhynchos) were foraging. Subadults watched adults steal food and participated in food-stealing with adults, which indicated interspecific kleptoparasitism may be a learned behavior. We suggest factors that may favor interspecific kleptoparasitism as a foraging strategy of Bald Eagles in obtaining waterfowl during severe winters.

Monitoring of raptors and their contamination levels in Norway.

PubMed

Gjershaug, Jan Ove; Kålås, John Atle; Nygård, Torgeir; Herzke, Dorte; Folkestad, Alv Ottar

2008-09-01

This article summarizes results from raptor monitoring and contamination studies in Norway of the golden eagle, gyrfalcon, white-tailed sea eagle, osprey, peregrine, and merlin. Golden eagle and gyrfalcon populations have been monitored since 1990 as part of the "Monitoring Programme for Terrestrial Ecosystems" (TOV). No long-term trend in the population size or productivity of golden eagle has been shown in any of the 5 study areas. The reproductive output of gyrfalcon is monitored in 3 areas. It is positively correlated with the populations of its main prey species, the rock ptarmigan and the willow ptarmigan. The white-tailed sea eagle population has been monitored since 1974 by the Norwegian Ornithological Society, and the population is increasing. The levels of pesticides and polychlorinated biphenyls are low in the eggs of both the golden eagle and the gyrfalcon, but elevated levels and effects on reproduction have been indicated for a coastal subpopulation of golden eagle. The pollutant levels in white-tailed sea eagle are lower than in the Baltic population of sea eagles, and shell thinning was never severe overall, but individual eggs have contained pollutant concentrations above critical levels. The levels of pollutants in the bird-eating falcons, peregrine, and merlin were higher than in other species. New emerging pollutants, like brominated diphenylethers and perfluorinated organic compounds, could be detected in all species. By incorporating available published and unpublished data, we were able to produce time trends for pollutants and shell thickness over 4 decades.

Using nestling feathers to assess spatial and temporal concentrations of mercury in bald eagles at Voyageurs National Park, Minnesota, USA

Treesearch

H. T. Pittman; W. W. Bowerman; L. H. Grim; Teryl Grubb; W. C. Bridges

2011-01-01

Bald eagles (Haliaeetus leucocephalus) have been utilized as a biosentinel of aquatic ecosystem health in the Great Lakes Region since the early 1960s. Bald eagle populations have been monitored at Voyageurs National Park (VNP), Minnesota, since 1973. For the past 20 years, researchers have collected feathers from nestling bald eagles to assess their dietary exposure...

KSC-06pd0912

NASA Image and Video Library

2006-05-19

KENNEDY SPACE CENTER, FLA. -- A female bald eagle looks over her surroundings from the perspective of a tall pine tree near S.R. 3 on Kennedy Space Center. There are a dozen active nests of bald eagles throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles that live along the coast and on major lakes and rivers feed mainly on fish. Photo credit: NASA/George Shelton

Residues of organochlorine pesticides and polychlorinated biphenyls and autopsy data for bald eagles, 1973-74

USGS Publications Warehouse

Prouty, R.M.; Reichel, W.L.; Locke, L.N.; Belisle, A.A.; Cromartie, E.; Kaiser, T.E.; Lamont, T.G.; Mulhern, B.M.; Swineford, D.M.

1977-01-01

Thirty-nine bald eagles found sick or dead in 13 States during 1969 and 1970 were analyzed for pesticide residues. Residues of DDE, dieldrin, polychlorinated biphenyls (PCB's), and mercury were detected in all bald eagle carcasses; DDD residues were detected in 38; DDT, heptachlor epoxide, and dichlorobenzophenone (DCBP) were detected less frequently. Six eagles contained possible lethal levels of dieldrin in the brain, and one contained a lethal concentration of DDE (385 ppm) in the brain together with 235 ppm of PCB's. Autopsy revealed that 18 bald eagles were illegally shot; other causes of death were impact injuries, electrocution, emaciation, and infectious diseases.

Electrochemical study of Type 304 and 316L stainless steels in simulated body fluids and cell cultures.

PubMed

Tang, Yee-Chin; Katsuma, Shoji; Fujimoto, Shinji; Hiromoto, Sachiko

2006-11-01

The electrochemical corrosion behaviour of Type 304 and 316L stainless steels was studied in Hanks' solution, Eagle's minimum essential medium (MEM), serum containing medium (MEM with 10% of fetal bovine serum) without cells, and serum containing medium with cells over a 1-week period. Polarization resistance measurements indicated that the stainless steels were resistant to Hanks' and MEM solutions. Type 304 was more susceptible to pitting corrosion than Type 316L in Hanks' and MEM solutions. The uniform corrosion resistance of stainless steels, determined by R(p), was lower in culturing medium than in Hanks' and MEM. The low corrosion resistance was due to surface passive film with less protective to reveal high anodic dissolution rate. When cells were present, the initial corrosion resistance was low, but gradually increased after 3 days, consistent with the trend of cell coverage. The presence of cells was found to suppress the cathodic reaction, that is, oxygen reduction, and increase the uniform corrosion resistance as a consequence. On the other hand, both Type 304 and 316L stainless steels became more susceptible to pitting corrosion when they were covered with cells.

The environmental dependence of H I in galaxies in the EAGLE simulations

NASA Astrophysics Data System (ADS)

Marasco, Antonino; Crain, Robert A.; Schaye, Joop; Bahé, Yannick M.; van der Hulst, Thijs; Theuns, Tom; Bower, Richard G.

2016-09-01

We use the EAGLE suite of cosmological hydrodynamical simulations to study how the H I content of present-day galaxies depends on their environment. We show that EAGLE reproduces observed H I mass-environment trends very well, while semi-analytic models typically overpredict the average H I masses in dense environments. The environmental processes act primarily as an on/off switch for the H I content of satellites with M* > 109 M⊙. At a fixed M*, the fraction of H I-depleted satellites increase with increasing host halo mass M200 in response to stronger environmental effects, while at a fixed M200 it decreases with increasing satellite M* as the gas is confined by deeper gravitational potentials. H I-depleted satellites reside mostly, but not exclusively, within the virial radius r200 of their host halo. We investigate the origin of these trends by focusing on three environmental mechanisms: ram pressure stripping by the intragroup medium, tidal stripping by the host halo and satellite-satellite encounters. By tracking back in time the evolution of the H I-depleted satellites, we find that the most common cause of H I removal is satellite encounters. The time-scale for H I removal is typically less than 0.5 Gyr. Tidal stripping occurs in haloes of M200 < 1014 M⊙ within 0.5 × r200, while the other processes act also in more massive haloes, generally within r200. Conversely, we find that ram pressure stripping is the most common mechanism that disturbs the H I morphology of galaxies at redshift z = 0. This implies that H I removal due to satellite-satellite interactions occurs on shorter time-scales than the other processes.

4. Historic American Buildings Survey, Henry Pleasants, History of the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Historic American Buildings Survey, Henry Pleasants, History of the Old Eagle School, John C. Winston Co., 1909 PHOTOCOPY. - Eagle School, Old Eagle School Road (State Route 543) (Tredyffrin Township), Strafford, Chester County, PA

3. Historic American Buildings Survey, Henry Pleasants, History of the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Historic American Buildings Survey, Henry Pleasants, History of the Old Eagle School, John C. Winston Co., 1909 PHOTOCOPY. - Eagle School, Old Eagle School Road (State Route 543) (Tredyffrin Township), Strafford, Chester County, PA

EAGLE: 'EAGLE'Is an' Algorithmic Graph Library for Exploration

DOE Office of Scientific and Technical Information (OSTI.GOV)

2015-01-16

The Resource Description Framework (RDF) and SPARQL Protocol and RDF Query Language (SPARQL) were introduced about a decade ago to enable flexible schema-free data interchange on the Semantic Web. Today data scientists use the framework as a scalable graph representation for integrating, querying, exploring and analyzing data sets hosted at different sources. With increasing adoption, the need for graph mining capabilities for the Semantic Web has emerged. Today there is no tools to conduct "graph mining" on RDF standard data sets. We address that need through implementation of popular iterative Graph Mining algorithms (Triangle count, Connected component analysis, degree distribution,more » diversity degree, PageRank, etc.). We implement these algorithms as SPARQL queries, wrapped within Python scripts and call our software tool as EAGLE. In RDF style, EAGLE stands for "EAGLE 'Is an' algorithmic graph library for exploration. EAGLE is like 'MATLAB' for 'Linked Data.'« less

KSC-06pd0473

NASA Image and Video Library

2006-03-11

KENNEDY SPACE CENTER, FLA. - A pair of mating bald eagles are spotted in their pine-tree nest near S.R. 3 on Kennedy Space Center. The nest, decades old, measures about 12 feet deep. The nest is one of a dozen active nests throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles which live along the coast and on major lakes and rivers feed mainly on fish. Bald eagles fish in both fresh and salt water. Because of the energy expended during hunting, an eagle has to spend a lot of time resting quietly. It's estimated that only one out of eighteen attacks are successful. Photo credit: NASA/Jim Grossmann

Lead Levels in Utah Eagles

NASA Astrophysics Data System (ADS)

Arnold, Michelle

2006-10-01

Lead is a health hazard to most animals, causing adverse effects to the nervous and reproductive systems if in sufficient quantity. Found in most fishing jigs and sinkers, as well as some ammunition used in hunting, this metal can poison wildlife such as eagles. Eagles are raptors, or predatory birds, and their lead exposure would most likely comes from their food -- a fish which has swallowed a sinker or lead shot in carrion (dead animal matter). As part of an ongoing project to investigate the environment lead levels in Utah, the bone lead levels in the wing bones of eagles have been measured for eagle carcasses found throughout Utah. The noninvasive technique of x-ray fluorescence was used, consisting of a Cd-109 radioactive source to activate lead atoms and a HPGe detector with digital electronics to collect the gamma spectra. Preliminary results for the eagles measured to date will be presented.

KSC-06pd0475

NASA Image and Video Library

2006-03-11

KENNEDY SPACE CENTER, FLA. - A bald eagle, spotted near S.R. 3 on Kennedy Space Center, begins its flight, perhaps scouting for food or heading for its nearby nest. There are a dozen active nests throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles which live along the coast and on major lakes and rivers feed mainly on fish. Bald eagles fish in both fresh and salt water. Because of the energy expended during hunting, an eagle has to spend a lot of time resting quietly. It's estimated that only one out of eighteen attacks are successful. Photo credit: NASA/Jim Grossmann

KSC-06pd0474

NASA Image and Video Library

2006-03-11

KENNEDY SPACE CENTER, FLA. - A bald eagle on the ground may be protecting its food or resting after a hunt for food. This one was spotted near S.R. 3 on Kennedy Space Center, close to its nest. The nest is one of a dozen active nests throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles which live along the coast and on major lakes and rivers feed mainly on fish. Bald eagles fish in both fresh and salt water. Because of the energy expended during hunting, an eagle has to spend a lot of time resting quietly. It's estimated that only one out of eighteen attacks are successful. Photo credit: NASA/Jim Grossmann

Long-term survival despite low genetic diversity in the critically endangered Madagascar fish-eagle

USGS Publications Warehouse

Johnson, J.A.; Tingay, R.E.; Culver, M.; Hailer, F.; Clarke, M.L.; Mindell, D.P.

2009-01-01

The critically endangered Madagascar fish-eagle (Haliaeetus vociferoides) is considered to be one of the rarest birds of prey globally and at significant risk of extinction. In the most recent census, only 222 adult individuals were recorded with an estimated total breeding population of no more than 100-120 pairs. Here, levels of Madagascar fish-eagle population genetic diversity based on 47 microsatellite loci were compared with its sister species, the African fish-eagle (Haliaeetus vocifer), and 16 of these loci were also characterized in the white-tailed eagle (Haliaeetus albicilla) and the bald eagle (Haliaeetus leucocephalus). Overall, extremely low genetic diversity was observed in the Madagascar fish-eagle compared to other surveyed Haliaeetus species. Determining whether this low diversity is the result of a recent bottleneck or a more historic event has important implications for their conservation. Using a Bayesian coalescent-based method, we show that Madagascar fish-eagles have maintained a small effective population size for hundreds to thousands of years and that its low level of neutral genetic diversity is not the result of a recent bottleneck. Therefore, efforts made to prevent Madagascar fish-eagle extinction should place high priority on maintenance of habitat requirements and reducing direct and indirect human persecution. Given the current rate of deforestation in Madagascar, we further recommend that the population be expanded to occupy a larger geographical distribution. This will help the population persist when exposed to stochastic factors (e.g. climate and disease) that may threaten a species consisting of only 200 adult individuals while inhabiting a rapidly changing landscape. ?? 2008 The Authors.

Pleistocene to historic shifts in bald eagle diets on the Channel Islands, California.

PubMed

Newsome, Seth D; Collins, Paul W; Rick, Torben C; Guthrie, Daniel A; Erlandson, Jon M; Fogel, Marilyn L

2010-05-18

Studies of current interactions among species, their prey, and environmental factors are essential for mitigating immediate threats to population viability, but the true range of behavioral and ecological flexibility can be determined only through research on deeper timescales. Ecological data spanning centuries to millennia provide important contextual information for long-term management strategies, especially for species that now are living in relict populations. Here we use a variety of methods to reconstruct bald eagle diets and local abundance of their potential prey on the Channel Islands from the late Pleistocene to the time when the last breeding pairs disappeared from the islands in the mid-20th century. Faunal and isotopic analysis of bald eagles shows that seabirds were important prey for immature/adult eagles for millennia before the eagles' local extirpation. In historic times (A.D. 1850-1950), however, isotopic and faunal data show that breeding bald eagles provisioned their chicks with introduced ungulates (e.g., sheep), which were locally present in high densities. Today, bald eagles are the focus of an extensive conservation program designed to restore a stable breeding population to the Channel Islands, but native and nonnative prey sources that were important for bald eagles in the past are either diminished (e.g., seabirds) or have been eradicated (e.g., introduced ungulates). In the absence of sufficient resources, a growing bald eagle population on the Channel Islands could expand its prey base to include carrion from local pinniped colonies, exert predation pressure on a recovering seabird population, and possibly prey on endangered island foxes.

Long-term survival despite low genetic diversity in the critically endangered Madagascar fish-eagle.

PubMed

Johnson, Jeff A; Tingay, Ruth E; Culver, Melanie; Hailer, Frank; Clarke, Michèle L; Mindell, David P

2009-01-01

The critically endangered Madagascar fish-eagle (Haliaeetus vociferoides) is considered to be one of the rarest birds of prey globally and at significant risk of extinction. In the most recent census, only 222 adult individuals were recorded with an estimated total breeding population of no more than 100-120 pairs. Here, levels of Madagascar fish-eagle population genetic diversity based on 47 microsatellite loci were compared with its sister species, the African fish-eagle (Haliaeetus vocifer), and 16 of these loci were also characterized in the white-tailed eagle (Haliaeetus albicilla) and the bald eagle (Haliaeetus leucocephalus). Overall, extremely low genetic diversity was observed in the Madagascar fish-eagle compared to other surveyed Haliaeetus species. Determining whether this low diversity is the result of a recent bottleneck or a more historic event has important implications for their conservation. Using a Bayesian coalescent-based method, we show that Madagascar fish-eagles have maintained a small effective population size for hundreds to thousands of years and that its low level of neutral genetic diversity is not the result of a recent bottleneck. Therefore, efforts made to prevent Madagascar fish-eagle extinction should place high priority on maintenance of habitat requirements and reducing direct and indirect human persecution. Given the current rate of deforestation in Madagascar, we further recommend that the population be expanded to occupy a larger geographical distribution. This will help the population persist when exposed to stochastic factors (e.g. climate and disease) that may threaten a species consisting of only 200 adult individuals while inhabiting a rapidly changing landscape.

Improved Chemically Defined Basal Medium (CMRL-1969) for Primary Monkey Kidney and Human Diploid Cells 1

PubMed Central

Healy, G. M.; Teleki, S.; Seefried, A. V.; Walton, M. J.; Macmorine, H. G.

1971-01-01

An improved tissue culture basal medium, CMRL-1969, supplemented with serum, has been evaluated by measuring the growth responses of primary cultures of trypsin-dispersed monkey kidney cells (PMKC) and of an established culture of a human diploid cell strain (HDCS). Medium H597, an early modification of medium 199 which has been used successfully in the preparation of poliomyelitis vaccine for 15 years, was used for comparison. In addition, parallel testing was done with Basal Medium Eagle (BME) widely used for the growth of HDCS. The improvements in basal medium CMRL-1969 are attributed to changes in amino acid concentrations, in vitamin composition, and, in particular, to enhanced buffering capacity. The latter has been achieved by the use of free-base amino acids and by increasing the dibasic sodium phosphate. The new medium has already been used to advantage for the production of polioviruses in PMKC where equivalent titers were obtained from cultures initiated with 70% of the number of cells required with earlier media. The population-doubling time was reduced in this system. Also, with small inocula of HDCS, the time required to obtain maximum cell yield was shorter with CMRL-1969 than with BME. Both media were supplemented with 10% calf serum. Maximum cell yields after repeated subcultivation in the new basal medium were greatly increased and the stability of the strain, as shown by chromosomal analysis, was not affected. Basal medium CMRL-1969 can be prepared easily in liquid or powdered form. PMID:4322279

KSC-06pd0913

NASA Image and Video Library

2006-05-19

KENNEDY SPACE CENTER, FLA. -- From a branch in a tall pine tree near S.R. 3 on Kennedy Space Center, a female bald eagle gives a wary look toward the photographer. There are a dozen active nests of bald eagles throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles that live along the coast and on major lakes and rivers feed mainly on fish. Photo credit: NASA/George Shelton

KSC-06pd0911

NASA Image and Video Library

2006-05-19

KENNEDY SPACE CENTER, FLA. -- From a branch in a tall pine tree near S.R. 3 on Kennedy Space Center , a female bald eagle strikes a familiar pose as she surveys her surroundings. There are a dozen active nests of bald eagles throughout the Merritt Island National Wildlife Refuge, which shares a boundary with the Center. Eagles' habitats are near lakes, rivers, marshes and seacoasts. Nests are masses of sticks, usually in the top of a tall tree. Even though they are fish eaters, bald eagles will take whatever prey is available and easiest to obtain. Bald eagles that live along the coast and on major lakes and rivers feed mainly on fish. Photo credit: NASA/George Shelton

Residues of organochlorine pesticides, polychlorinated biphenyls, and mercury and autopsy data for bald eagles, 1969 and 1970

USGS Publications Warehouse

Belisle, Andre A.; Reichel, William L.; Locke, Louis N.; Lamont, Thair; Mulhern, Bernard M.; Prouty, Richard M.; DeWolf, Robert B.; Cromartie, Eugene

1972-01-01

Thirty-nine bald eagles found sick or dead in 13 States during 1969 and 1970 were analyzed for pesticide residues. Residues of DDE, dieldrin, polychlorinated biphenyls (PCB's), and mercury were detected in all bald eagle carcasses; DDD residues were detected in 38; DDT, heptachlor epoxide, and dichlorobenzophenone (DCBP) were detected less frequently. Six eagles contained possible lethal levels of dieldrin in the brain, and one contained a lethal concentration of DDE (385 ppm) in the brain together with 235 ppm of PCB's. Autopsy revealed that 18 bald eagles were illegally shot; other causes of death were impact injuries, electrocution, emaciation, and infectious diseases.

Can the Λ CDM model reproduce MOND-like behavior?

NASA Astrophysics Data System (ADS)

Dai, De-Chang; Lu, Chunyu

2017-12-01

It is usually believed that MOND can describe the galactic rotational curves with only baryonic matter and without any dark matter very well, while the Λ CDM model is expected to have difficulty in reproducing MOND-like behavior. Here, we use EAGLE's data to learn whether the Λ CDM model can reproduce MOND-like behavior. EAGLE's simulation result clearly reproduces the MOND-like behavior for ab⪆10-12 m/s 2 at z =0 , although the acceleration constant, a0, is a little larger than the observational data indicate. We find that a0 increases with the redshift in a way different from what Milgrom proposed (a0∝H ). Therefore, while galaxy rotation curves can be fitted by MOND's empirical function in the Λ CDM model, there is no clear connection between a0 and the Hubble constant. We also find that a0 at z ⪆1 is well separated from a0 at z =0 . Once we have enough galaxies observed at high redshifts, we will be able to rule out the modified gravity model based on MOND-like empirical function with a z -independent a0.

Disease-mediated bottom-up regulation: An emergent virus affects a keystone prey, and alters the dynamics of trophic webs

PubMed Central

Monterroso, Pedro; Garrote, Germán; Serronha, Ana; Santos, Emídio; Delibes-Mateos, Miguel; Abrantes, Joana; Perez de Ayala, Ramón; Silvestre, Fernando; Carvalho, João; Vasco, Inês; Lopes, Ana M.; Maio, Elisa; Magalhães, Maria J.; Mills, L. Scott; Esteves, Pedro J.; Simón, Miguel Ángel; Alves, Paulo C.

2016-01-01

Emergent diseases may alter the structure and functioning of ecosystems by creating new biotic interactions and modifying existing ones, producing cascading processes along trophic webs. Recently, a new variant of the rabbit haemorrhagic disease virus (RHDV2 or RHDVb) arguably caused widespread declines in a keystone prey in Mediterranean ecosystems - the European rabbit (Oryctolagus cuniculus). We quantitatively assess the impact of RHDV2 on natural rabbit populations and in two endangered apex predator populations: the Iberian lynx (Lynx pardinus) and the Spanish Imperial eagle (Aquila adalberti). We found 60–70% declines in rabbit populations, followed by decreases of 65.7% in Iberian lynx and 45.5% in Spanish Imperial eagle fecundities. A revision of the web of trophic interactions among rabbits and their dependent predators suggests that RHDV2 acts as a keystone species, and may steer Mediterranean ecosystems to management-dependent alternative states, dominated by simplified mesopredator communities. This model system stresses the importance of diseases as functional players in the dynamics of trophic webs. PMID:27796353

Landscapes for Energy and Wildlife: Conservation Prioritization for Golden Eagles across Large Spatial Scales

PubMed Central

Tack, Jason D.; Fedy, Bradley C.

2015-01-01

Proactive conservation planning for species requires the identification of important spatial attributes across ecologically relevant scales in a model-based framework. However, it is often difficult to develop predictive models, as the explanatory data required for model development across regional management scales is rarely available. Golden eagles are a large-ranging predator of conservation concern in the United States that may be negatively affected by wind energy development. Thus, identifying landscapes least likely to pose conflict between eagles and wind development via shared space prior to development will be critical for conserving populations in the face of imposing development. We used publically available data on golden eagle nests to generate predictive models of golden eagle nesting sites in Wyoming, USA, using a suite of environmental and anthropogenic variables. By overlaying predictive models of golden eagle nesting habitat with wind energy resource maps, we highlight areas of potential conflict among eagle nesting habitat and wind development. However, our results suggest that wind potential and the relative probability of golden eagle nesting are not necessarily spatially correlated. Indeed, the majority of our sample frame includes areas with disparate predictions between suitable nesting habitat and potential for developing wind energy resources. Map predictions cannot replace on-the-ground monitoring for potential risk of wind turbines on wildlife populations, though they provide industry and managers a useful framework to first assess potential development. PMID:26262876

Landscapes for energy and wildlife: conservation prioritization for golden eagles across large spatial scales

USGS Publications Warehouse

Tack, Jason D.; Fedy, Bradley C.

2015-01-01

Proactive conservation planning for species requires the identification of important spatial attributes across ecologically relevant scales in a model-based framework. However, it is often difficult to develop predictive models, as the explanatory data required for model development across regional management scales is rarely available. Golden eagles are a large-ranging predator of conservation concern in the United States that may be negatively affected by wind energy development. Thus, identifying landscapes least likely to pose conflict between eagles and wind development via shared space prior to development will be critical for conserving populations in the face of imposing development. We used publically available data on golden eagle nests to generate predictive models of golden eagle nesting sites in Wyoming, USA, using a suite of environmental and anthropogenic variables. By overlaying predictive models of golden eagle nesting habitat with wind energy resource maps, we highlight areas of potential conflict among eagle nesting habitat and wind development. However, our results suggest that wind potential and the relative probability of golden eagle nesting are not necessarily spatially correlated. Indeed, the majority of our sample frame includes areas with disparate predictions between suitable nesting habitat and potential for developing wind energy resources. Map predictions cannot replace on-the-ground monitoring for potential risk of wind turbines on wildlife populations, though they provide industry and managers a useful framework to first assess potential development.

Lead exposure in raptors from Japan and source identification using Pb stable isotope ratios.

PubMed

Ishii, Chihiro; Nakayama, Shouta M M; Ikenaka, Yoshinori; Nakata, Hokuto; Saito, Keisuke; Watanabe, Yukiko; Mizukawa, Hazuki; Tanabe, Shinsuke; Nomiyama, Kei; Hayashi, Terutake; Ishizuka, Mayumi

2017-11-01

Lead (Pb) poisoning is widespread among raptors and water birds. In Japan, fragments of Pb ammunition are still found in endangered eagles although more than 10 years have passed since legislation regarding use of Pb ammunition was introduced. This study was performed to investigate Pb exposure in raptors from various locations in Japan. We measured hepatic and renal Pb concentrations and hepatic Pb isotope ratios of Steller's sea eagles (Haliaeetus pelagicus), white-tailed sea eagles (Haliaeetus albicilla), golden eagles (Aquila chrysaetos), and 13 other species (total 177 individuals) that were found dead, as well as blood samples from three eagles found in a weakened state during 1993-2015 from Hokkaido (northern part), Honshu (the main island), and Shikoku (a southern island) of Japan. In the present study in Hokkaido, one quarter of the sea eagles showed a high Pb concentration, suggesting exposure to abnormally high Pb levels and Pb poisoning. Pb isotope ratios indicated that endangered Steller's sea eagle and white-tailed sea eagle were poisoned by Pb ammunition that was used illegally in Hokkaido. In other areas of Japan, both surveillance and regulations were less extensive than in Hokkaido, but Pb poisoning in raptors was also noted. Therefore, Pb poisoning is still a serious problem in raptors in various areas of Japan due to accidental ingestion of materials containing Pb, especially Pb ammunition. Copyright © 2017. Published by Elsevier Ltd.

Landscapes for Energy and Wildlife: Conservation Prioritization for Golden Eagles across Large Spatial Scales.

PubMed

Tack, Jason D; Fedy, Bradley C

2015-01-01

Proactive conservation planning for species requires the identification of important spatial attributes across ecologically relevant scales in a model-based framework. However, it is often difficult to develop predictive models, as the explanatory data required for model development across regional management scales is rarely available. Golden eagles are a large-ranging predator of conservation concern in the United States that may be negatively affected by wind energy development. Thus, identifying landscapes least likely to pose conflict between eagles and wind development via shared space prior to development will be critical for conserving populations in the face of imposing development. We used publically available data on golden eagle nests to generate predictive models of golden eagle nesting sites in Wyoming, USA, using a suite of environmental and anthropogenic variables. By overlaying predictive models of golden eagle nesting habitat with wind energy resource maps, we highlight areas of potential conflict among eagle nesting habitat and wind development. However, our results suggest that wind potential and the relative probability of golden eagle nesting are not necessarily spatially correlated. Indeed, the majority of our sample frame includes areas with disparate predictions between suitable nesting habitat and potential for developing wind energy resources. Map predictions cannot replace on-the-ground monitoring for potential risk of wind turbines on wildlife populations, though they provide industry and managers a useful framework to first assess potential development.

Decellularized amnion scaffold with activated PRP: a new paradigm dressing material for burn wound healing.

PubMed

Kshersagar, Jeevitaa; Kshirsagar, Ravi; Desai, Shashikant; Bohara, Raghvendra; Joshi, Meghnad

2018-03-05

Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD). Decellularized amnion samples were analyzed by haematoxylin-eosin staining (HE), Alcian blue pH 1 (AB-pH-1), 4,6-diamnionidino-2-phenylindol (DAPI), Massion's trichrome stain, DNA quantification, mechanical testing and scanning electron microscopy (SEM). Histological analysis showed complete removal of cellular components and the histoarchitecture of scaffold remained intact. Amnion scaffold activated with platelet rich plasma (PRP) and calcium chloride composition supported better adherence to the wound than amnion alone. Only single application showed good healing. In vivo assessment of activated amnion revealed stable dressing. It has good promising outcome. At day 7, histologically the wounds treated with activated amnion were almost closed without scarring and showed well differentiated epidermis, proliferation of keratinocytes, hair follicles and basement membrane as compared to controls and silver nitrate gel dressings in a mouse (Mus musculus). Cryopreservation had no adverse effect on the mechanical properties of the amnion scaffold. Cryopreservation of decellularized amnion by Dulbecco's modified eagle medium (DMEM) was expected to prepare off-the-shelf skin substitutes and preserve them to be immediately available upon request of patients' needs.

Ethanol exposure induces oxidative stress and impairs nitric oxide availability in the human placental villi: a possible mechanism of toxicity.

PubMed

Kay, H H; Grindle, K M; Magness, R R

2000-03-01

We undertook this investigation to explore the effects of ethanol exposure on nitric oxide synthase levels and nitric oxide release. Our hypothesis was that ethanol exposure modifies nitric oxide activity within the placenta as a result of oxidative stress. Four 10-g samples of term normal human placental villous tissue were perifused with nonrecirculating Dulbecco's modified Eagle's medium and 25-mmol/L N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid] with 0-, 50-, 100-, or 200-mmol/L ethanol. After 2 hours of exposure, tissue was removed, fixed, and frozen for analysis. Immunohistochemical analysis was performed for subtype I or neuronal nitric oxide synthase (nNOS), subtype II or inducible nitric oxide synthase (iNOS), and subtype III or endothelial nitric oxide synthase (eNOS) localization. Western blot analysis was performed for eNOS quantitation. Cyclic guanosine monophosphate and copper-zinc superoxide dismutase levels were measured by electroimmunoassay and kinetic assay, respectively. Nitric oxide release was analyzed by a Sievers nitric oxide analyzer. Immunohistochemical examination confirmed that only eNOS was localized to the syncytiotrophoblasts. After ethanol exposure, eNOS protein expression increased 2.5- to 3.0-fold over that of the control. Tissue cyclic guanosine monophosphate content and nitric oxide release into the effluent were decreased, whereas superoxide dismutase levels were increased at higher ethanol levels (P <.05). Ethanol exposure appears to induce oxidative stress, which may account for the decreased nitric oxide release, because nitric oxide may be shunted toward scavenging free radicals. Increased eNOS protein expression may be a response to the increased demand for nitric oxide. Decreased nitric oxide availability could adversely affect placental blood flow regulation, which could, in turn, account for the growth restriction seen in ethanol-exposed fetuses.

Geology and sequence stratigraphy of undiscovered oil and gas resources in conventional and continuous petroleum systems in the Upper Cretaceous Eagle Ford Group and related strata, U.S. Gulf Coast Region

USGS Publications Warehouse

Dubiel, Russell F.; Pearson, Ofori N.; Pitman, Janet K.; Pearson, Krystal M.; Kinney, Scott A.

2012-01-01

The U.S. Geological Survey (USGS) recently assessed the technically recoverable undiscovered oil and gas onshore and in State waters of the Gulf Coast region of the United States. The USGS defined three assessment units (AUs) with potential undiscovered conventional and continuous oil and gas resources in Upper Cretaceous (Cenomanian to Turonian) strata of the Eagle Ford Group and correlative rocks. The assessment is based on geologic elements of a total petroleum system, including hydrocarbon source rocks (source rock maturation, hydrocarbon generation and migration), reservoir rocks (sequence stratigraphy and petrophysical properties), and traps (formation, timing, and seals). Conventional oil and gas undiscovered resources are in updip sandstone reservoirs in the Upper Cretaceous Tuscaloosa and Woodbine Formations (or Groups) in Louisiana and Texas, respectively, whereas continuous oil and continuous gas undiscovered resources reside in the middip and downdip Upper Cretaceous Eagle Ford Shale in Texas and the Tuscaloosa marine shale in Louisiana. Conventional resources in the Tuscaloosa and Woodbine are included in the Eagle Ford Updip Sandstone Oil and Gas AU, in an area where the Eagle Ford Shale and Tuscaloosa marine shale display vitrinite reflectance (Ro) values less than 0.6%. The continuous Eagle Ford Shale Oil AU lies generally south of the conventional AU, is primarily updip of the Lower Cretaceous shelf edge, and is defined by thermal maturity values within shales of the Eagle Ford and Tuscaloosa that range from 0.6 to 1.2% Ro. Similarly, the Eagle Ford Shale Gas AU is defined downdip of the shelf edge where source rocks have Ro values greater than 1.2%. For undiscovered oil and gas resources, the USGS assessed means of: 1) 141 million barrels of oil (MMBO), 502 billion cubic feet of natural gas (BCFG), and 4 million barrels of natural gas liquids (MMBNGL) in the Eagle Ford Updip Sandstone Oil and Gas AU; 2) 853 MMBO, 1707 BCFG, and 34 MMBNGL in the Eagle Ford Shale Oil AU; and 3) 50,219 BCFG and 2009 MMBNGL in the Eagle Ford Shale Gas AU.

50 CFR 22.26 - Permits for eagle take that is associated with, but not the purpose of, an activity.

Code of Federal Regulations, 2011 CFR

2011-10-01

... of the Region in which the disturbance would occur—Attention: Migratory Bird Permit Office. You can... the eagle's nest, roost, or foraging perches; and (iii) Whether alternative suitable eagle nesting...

Tuberous sclerosis: aberrant metabolism of ornithine, proline and glutamate in cultured fibroblasts.

PubMed

Tanaka, H; Nakazawa, K; Arima, M; Hayashi, A

1987-01-01

To investigate aberrant metabolism of proline (Pro) and its precursors in tuberous sclerosis (TS), 6, 7 and 5 strains of control, TS (normal skin) and TS (tumor) fibroblasts, respectively, were cultured in Eagle's MEM containing dialyzed fetal bovine serum with or without 0.1 mM ornithine (Orn). Ornithine aminotransferase (OAT) activity was decreased in TS, especially in TS (tumor) after mild sonication treatment. The yield of the OAT protein was inhibited in TS (tumor) when cultured without Orn. Free glutamate (Glu) in the medium was significantly increased in TS (tumor). Free proline (Pro) in cells was significantly decreased in TS (tumor) when cultured with Orn, but protein-bound Pro was not. The relative concentration of free Glu to glutamine (Gln) in the medium and that of free Glu to Pro in cells cultured with Orn were increased in TS (tumor). These results suggest that the requirement for Orn, increased turnover of Pro to Glu and increased elimination of Glu into the medium occur in TS (tumor). Aberrant regulation or turnover of Pro and Glu metabolism may occur in TS, especially in tumor cells.

Application of the isotope-dilution principle to the analysis of factors affecting the incorporation of [3H]uridine and [3H]cytidine into cultured lymphocytes. Evaluation of pools in serum and culture media

PubMed Central

Forsdyke, D. R.

1971-01-01

1. Rat lymph-node cells were incubated in serum and medium 199 with [5-3H]uridine or [5-3H]cytidine and acid-precipitable radioactivity was measured. Results were interpreted in terms of an isotope-dilution model. 2. Both serum and medium 199 contained pools that inhibited radioactive labelling in a competitive manner. The serum activity was diffusible and inhibited labelling with [3H]cytidine more than with [3H]uridine; in these respects the activity resembled cytidine (14μm). 3. The pools in serum and plasma were the same size; however, the rate of labelling was greater in plasma, owing to a diffusible factor. 4. Paradoxically, relatively simple media (Earle's salts and Eagle's minimum essential) appeared to have a larger pool than the more complex pyrimidine-containing medium 199; this suggests a contribution to the pool by cells in the simple media. 5. In the absence of pools the average cell was capable of incorporating 2000 radioactive nucleoside molecules/s. PMID:4947658

Spizaetus hawk-eagles as predators of arboreal colobines.

PubMed

Fam, S D; Nijman, V

2011-04-01

The predation pressure put on primates by diurnal birds of prey differs greatly between continents. Africa and South America have specialist raptors (e.g. crowned hawk-eagle Stephanoaetus coronatus and harpy eagle Harpia harpyja) whereas in Asia the only such specialist's (Philippine eagle Pithecophaga jefferyi) distribution is largely allopatric with primates. The almost universal absence of polyspecific groups in Asia (common in Africa and South America) may indicate reduced predation pressure. As such there is almost no information on predation pressures on primates in Asia by raptors. Here we report successful predation of a juvenile banded langur Presbytis femoralis (~2 kg) by a changeable hawk-eagle Spizaetus cirrhatus. The troop that was attacked displayed no signs of being alarmed, and no calls were made before the event. We argue that in insular Southeast Asia, especially, large Spizaetus hawk-eagles (~2 kg) are significant predators of arboreal colobines. Using data on the relative size of sympatric Spizaetus hawk-eagles and colobines we make predictions on where geographically we can expect the highest predation pressure (Thai-Malay Peninsula) and which colobines are least (Nasalis larvatus, Trachypithecus auratus, P. thomasi) and most (P. femoralis, T. cristatus) affected.

Patterns of spatial distribution of golden eagles across North America: How do they fit into existing landscape-scale mapping systems?

USGS Publications Warehouse

Brown, Jessi L.; Bedrosian, Bryan; Bell, Douglas A.; Braham, Melissa A.; Cooper, Jeff; Crandall, Ross H.; DiDonato, Joe; Domenech, Robert; Duerr, Adam E.; Katzner, Todd; Lanzone, Michael J.; LaPlante, David W.; McIntyre, Carol L.; Miller, Tricia A.; Murphy, Robert K.; Shreading, Adam; Slater, Steven J.; Smith, Jeff P.; Smith, Brian W.; Watson, James W.; Woodbridge, Brian

2017-01-01

Conserving wide-ranging animals requires knowledge about their year-round movements and resource use. Golden Eagles (Aquila chrysaetos) exhibit a wide range of movement patterns across North America. We combined tracking data from 571 Golden Eagles from multiple independent satellite-telemetry projects from North America to provide a comprehensive look at the magnitude and extent of these movements on a continental scale. We compared patterns of use relative to four alternative administrative and ecological mapping systems, namely Bird Conservation Regions (BCRs), U.S. administrative migratory bird flyways, Migratory Bird Joint Ventures, and Landscape Conservation Cooperatives. Our analyses suggested that eagles initially captured in eastern North America used space differently than those captured in western North America. Other groups of eagles that exhibited distinct patterns in space use included long-distance migrants from northern latitudes, and southwestern and Californian desert residents. There were also several groupings of eagles in the Intermountain West. Using this collaborative approach, we have identified large-scale movement patterns that may not have been possible with individual studies. These results will support landscape-scale conservation measures for Golden Eagles across North America.

DefenseLink.mil - Special Report - Symbols of America

Science.gov Websites

United States and the selection of the golden eagle as the national bird. Over the years, other symbols Liberty Bald Eagle Bald Eagle Uncle Sam Uncle Sam The Capitol The Capitol The Great Seal The Great Seal

Caribou antlers as nest materials for golden eagles in northwestern Alaska

USGS Publications Warehouse

Ellis, D.H.; Bunn, R.L.

1998-01-01

There are few published records of antlers in golden eagle (Aquila chrysaetos) nests. This note reports extensive use of caribou (Rangifer tarandus) antlers in three golden eagle nests in the Cape Kruzenstern region of northwestern Alaska. The importance of antlers to this population of eagles can be explained at least in part by (1) the lack of suitable woody vegetation on the open tundra, (2) the similarity of antlers to sticks, and (3) the abundance of antlers, especially cow caribou antlers, in the region.

Modeling Late-Summer Distribution of Golden Eagles (Aquila chrysaetos) in the Western United States

PubMed Central

Gardner, Grant

2016-01-01

Increasing development across the western United States (USA) elevates concerns about effects on wildlife resources; the golden eagle (Aquila chrysaetos) is of special concern in this regard. Knowledge of golden eagle abundance and distribution across the western USA must be improved to help identify and conserve areas of major importance to the species. We used distance sampling and visual mark-recapture procedures to estimate golden eagle abundance from aerial line-transect surveys conducted across four Bird Conservation Regions in the western USA between 15 August and 15 September in 2006–2010, 2012, and 2013. To assess golden eagle-habitat relationships at this scale, we modeled counts of golden eagles seen during surveys in 2006–2010, adjusted for probability of detection, and used land cover and other environmental factors as predictor variables within 20-km2 sampling units randomly selected from survey transects. We found evidence of positive relationships between intensity of use by golden eagles and elevation, solar radiation, and mean wind speed, and of negative relationships with the proportion of landscape classified as forest or as developed. The model accurately predicted habitat use observed during surveys conducted in 2012 and 2013. We used the model to construct a map predicting intensity of use by golden eagles during late summer across our ~2 million-km2 study area. The map can be used to help prioritize landscapes for conservation efforts, identify areas where mitigation efforts may be most effective, and identify regions for additional research and monitoring. In addition, our map can be used to develop region-specific (e.g., state-level) density estimates based on the latest information on golden eagle abundance from a late-summer survey and aid designation of geographic management units for the species. PMID:27556735

Modeling Late-Summer Distribution of Golden Eagles (Aquila chrysaetos) in the Western United States.

PubMed

Nielson, Ryan M; Murphy, Robert K; Millsap, Brian A; Howe, William H; Gardner, Grant

2016-01-01

Increasing development across the western United States (USA) elevates concerns about effects on wildlife resources; the golden eagle (Aquila chrysaetos) is of special concern in this regard. Knowledge of golden eagle abundance and distribution across the western USA must be improved to help identify and conserve areas of major importance to the species. We used distance sampling and visual mark-recapture procedures to estimate golden eagle abundance from aerial line-transect surveys conducted across four Bird Conservation Regions in the western USA between 15 August and 15 September in 2006-2010, 2012, and 2013. To assess golden eagle-habitat relationships at this scale, we modeled counts of golden eagles seen during surveys in 2006-2010, adjusted for probability of detection, and used land cover and other environmental factors as predictor variables within 20-km2 sampling units randomly selected from survey transects. We found evidence of positive relationships between intensity of use by golden eagles and elevation, solar radiation, and mean wind speed, and of negative relationships with the proportion of landscape classified as forest or as developed. The model accurately predicted habitat use observed during surveys conducted in 2012 and 2013. We used the model to construct a map predicting intensity of use by golden eagles during late summer across our ~2 million-km2 study area. The map can be used to help prioritize landscapes for conservation efforts, identify areas where mitigation efforts may be most effective, and identify regions for additional research and monitoring. In addition, our map can be used to develop region-specific (e.g., state-level) density estimates based on the latest information on golden eagle abundance from a late-summer survey and aid designation of geographic management units for the species.

Comparison of arsenic, cadmium, chromium, lead, manganese, mercury and selenium in feathers in bald eagle (Haliaeetus leucocephalus), and comparison with common eider (Somateria mollissima), glaucous-winged gull (Larus glaucescens), pigeon guillemot (Cepphus columba), and tufted puffin (Fratercula cirrhata) from the Aleutian Chain of Alaska

PubMed Central

Burger, Joanna; Gochfeld, Michael

2014-01-01

There is an abundance of field data for levels of metals from a range of places, but relatively few from the North Pacific Ocean and Bering Sea. In this paper we examine the levels of arsenic, cadmium, chromium, lead, manganese, mercury and selenium in feathers from common eiders (Somateria mollissima), glaucous-winged gulls (Larus glaucescens), pigeon guillemots (Cepphus columba), tufted puffins (Fratercula cirrhata) and bald eagles (Haliaeetus leucocephalus) from the Aleutian Chain of Alaska. Our primary objective was to test the hypothesis that there are no trophic levels relationships for arsenic, cadmium, chromium, lead, manganese, mercury and selenium among these five species of birds breeding in the marine environment of the Aleutians. There were significant interspecific differences in all metal levels. As predicted bald eagles had the highest levels of arsenic, chromium, lead, and manganese, but puffins had the highest levels of selenium, and pigeon guillemot had higher levels of mercury than eagles (although the differences were not significant). Common eiders, at the lowest trophic level had the lowest levels of some metals (chromium, mercury and selenium). However, eiders had higher levels than all other species (except eagles) for arsenic, cadmium, lead, and manganese. Levels of lead were higher in breast than in wing feathers of bald eagles. Except for lead, there were no significant differences in metal levels in feathers of bald eagles nesting on Adak and Amchitka Island; lead was higher on Adak than Amchitka. Eagle chicks tended to have lower levels of manganese than older eagles. PMID:18521716

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sinclair, Karin; DeGeorge, Elise

The Bald and Golden Eagle Protection Act (BGEPA) prohibits the 'take' of these birds. The act defines take as to 'pursue, shoot, shoot at, poison, wound, kill, capture, trap, collect, destroy, molest or disturb.' The 2009 Eagle Permit Rule (74 FR 46836) authorizes the U.S. Fish and Wildlife Service (USFWS) to issue nonpurposeful (i.e., incidental) take permits, and the USFWS 2013 Eagle Conservation Plan Guidance provides a voluntary framework for issuing programmatic take permits to wind facilities that incorporate scientifically supportable advanced conservation practices (ACPs). Under these rules, the Service can issue permits that authorize individual instances of take ofmore » bald and golden eagles when the take is associated with, but not the purpose of, an otherwise lawful activity, and cannot practicably be avoided. To date, the USFWS has not approved any ACPs, citing the lack of evidence for 'scientifically supportable measures.' The Eagle Detection and Deterrents Research Gaps and Solutions Workshop was convened at the National Renewable Energy Laboratory in December 2015 with a goal to comprehensively assess the current state of technologies to detect and deter eagles from wind energy sites and the key gaps concerning reducing eagle fatalities and facilitating permitting under the BGEPA. During the workshop, presentations and discussions focused primarily on existing knowledge (and limitations) about the biology of eagles as well as technologies and emerging or novel ideas, including innovative applications of tools developed for use in other sectors, such as the U.S. Department of Defense and aviation. The main activity of the workshop was the breakout sessions, which focused on the current state of detection and deterrent technologies and novel concepts/applications for detecting and minimizing eagle collisions with wind turbines. Following the breakout sessions, participants were asked about their individual impressions of the relative priority of each of the existing and novel ideas.« less

Reproductive characteristics of migratory golden eagles in Denali National Park, Alaska

USGS Publications Warehouse

McIntyre, Carol L.; Adams, Layne G.

1999-01-01

We describe reproductive characteristics of Golden Eagles (Aquila chrysaetos) breeding in Denali National Park, Alaska during an entire snowshoe hare (Lepus americanus) cycle, 1988-1997. Data on nesting eagles were collected at 58 to 72 nesting areas annually using two aerial surveys. Surveys were conducted during the incubation period to determine occupancy and nesting activities and late in the nestling period to count nestlings and determine nesting success. Annual occupancy rates of nesting areas did not vary significantly, whereas laying rates, success rates, and mean brood size varied significantly over the study period. Fledgling production for the study population varied sevenfold during the ten-year period. Laying rates, mean brood size, and overall population productivity were significantly correlated with abundance of cyclic snowshoe hare and Willow Ptarmigan (Lugopus lagopus) populations. Reproductive rates of Golden Eagles in Denali were similar to those of Golden Eagles from other high latitude study areas in North America, but lower than for Golden Eagles from temperate zone study areas in North America.

Nesting by Golden Eagles on the North Slope of the Brooks Range in Northeastern Alaska

USGS Publications Warehouse

Young, Donald D.; McIntyre, Carol L.; Bente, Peter J.; McCabe, Thomas R.; Ambrose, Robert E.

1995-01-01

Twenty-two Golden Eagle (Aquila chrysaetos) nesting territories and 31 occupied eagle nests were documented on the north slope of the Brooks Range in northeastern Alaska, 1988-1990, in an area previously thought to be marginal breeding habitat for eagles. The mean number of young/successful nest was 1.25 in 1988, 1.27 in 1989, and 1.13 in 1990; means did not differ significantly among years. Eighty percent (20/25) of the nestlings for which age was estimated were assumed to have successfully fledged. Nesting success was 79% (11/14) in 1989, the only year nesting success could be determined. Laying dates ranged from 23 March (1990) to 11 May (1989) with mean estimated laying dates differing significantly among years. Annual variation in nesting phenology coincided with annual differences in snow accumulations during spring. These results indicate that Golden Eagles consistently and successfully breed at the northern extent of their range in Alaska, although, productivity may be lower than that for eagles at more southern latitudes.

Flight Testing of Guidance, Navigation and Control Systems on the Mighty Eagle Robotic Lander Testbed

NASA Technical Reports Server (NTRS)

Hannan, Mike; Rickman, Doug; Chavers, Greg; Adam, Jason; Becker, Chris; Eliser, Joshua; Gunter, Dan; Kennedy, Logan; O'Leary, Patrick

2015-01-01

During 2011 a series of progressively more challenging flight tests of the Mighty Eagle autonomous terrestrial lander testbed were conducted primarily to validate the GNC system for a proposed lunar lander. With the successful completion of this GNC validation objective the opportunity existed to utilize the Mighty Eagle as a flying testbed for a variety of technologies. In 2012 an Autonomous Rendezvous and Capture (AR&C) algorithm was implemented in flight software and demonstrated in a series of flight tests. In 2012 a hazard avoidance system was developed and flight tested on the Mighty Eagle. Additionally, GNC algorithms from Moon Express and a MEMs IMU were tested in 2012. All of the testing described herein was above and beyond the original charter for the Mighty Eagle. In addition to being an excellent testbed for a wide variety of systems the Mighty Eagle also provided a great learning opportunity for many engineers and technicians to work a flight program.

KSC-07pd3396

NASA Image and Video Library

2007-11-19

KENNEDY SPACE CENTER, FLA. -- A bald eagle perches on a pole on Launch Pad 39A, seemingly monitoring the instruction of the space shuttle Atlantis STS-122 crew on slidewire basket operation, part of the emergency exit system on the fixed service structure of the pad. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Its natural domain is from Alaska to Baja, California, and from Maine to Florida. Bald eagles have a presence in every U. S. state except Hawaii. There are a dozen eagle nests in both Kennedy Space Center and the Merritt Island National Wildlife Refuge, which surrounds Kennedy. Bald eagles live near large bodies of open water such as lakes, marshes, seacoasts and rivers, where there are plenty of fish to eat and tall trees for nesting and roosting. Bald eagles feed primarily on fish, but also eat small animals (ducks, coots, muskrats, turtles, rabbits, snakes, etc.). Photo credit: NASA/Kim Shiflett

Bald eagle predation on common loon egg

USGS Publications Warehouse

DeStefano, Stephen; McCarthy, Kyle P.; Laskowski, Tom

2010-01-01

The Common Loon (Gavia immer) must defend against many potential egg predators during incubation, including corvids, Herring Gulls (Larus argentatus), raccoons (Procyon lotor), striped skunk (Mephitis mephitis), fisher (Martes pennanti), and mink (Neovison vison) (McIntyre 1988, Evers 2004, McCann et al. 2005). Bald Eagles (Haliaeetus leucocephalus) have been documented as predators of both adult Common Loons and their chicks (Vliestra and Paruk 1997, Paruk et al. 1999, Erlandson et al. 2007, Piper et al. 2008). In Wisconsin, where nesting Bald Eagles are abundant (>1200 nesting pairs, >1 young/pair/year), field biologists observed four instances of eagle predation of eggs in loon nests during the period 2002–2004 (M. Meyer pers. comm.). In addition, four cases of eagle predation of incubating adult loons were inferred from evidence found at the loon nest (dozens of plucked adult loon feathers, no carcass remains) and/or loon leg, neck, and skull bones beneath two active eagle nests, including leg bones containing the bands of the nearby (<25 m) incubating adult loon. However, although loon egg predation has been associated with Bald Eagles, predation events have yet to be described in peer-reviewed literature. Here we describe a photographic observation of predation on a Common Loon egg by an immature Bald Eagle as captured by a nest surveillance video camera on Lake Umbagog, a large lake (32 km2) at Umbagog National Wildlife Refuge (UNWR) in Maine.

Boundary of the Eagle River Watershed Valley-Fill Aquifer, Eagle County, North-Central Colorado, 2006-2007

USGS Publications Warehouse

Rupert, Michael G.; Plummer, Niel

2009-01-01

This vector data set delineates the approximate boundary of the Eagle River watershed valley-fill aquifer (ERWVFA). This data set was developed by a cooperative project between the U.S. Geological Survey, Eagle County, the Eagle River Water and Sanitation District, the Town of Eagle, the Town of Gypsum, and the Upper Eagle Regional Water Authority. This project was designed to evaluate potential land-development effects on groundwater and surface-water resources so that informed land-use and water management decisions can be made. The boundary of the ERWVFA was developed by combining information from two data sources. The first data source was a 1:250,000-scale geologic map of the Leadville quadrangle developed by Day and others (1999). The location of Quaternary sediments was used as a first approximation of the ERWVFA. The boundary of the ERWVFA was further refined by overlaying the geologic map with Digital Raster Graphic (DRG) scanned images of 1:24,000 topographic maps (U.S. Geological Survey, 2001). Where appropriate, the boundary of the ERWVFA was remapped to correspond with the edge of the valley-fill aquifer marked by an abrupt change in topography at the edge of the valley floor throughout the Eagle River watershed. The boundary of the ERWVFA more closely resembles a hydrogeomorphic region presented by Rupert (2003, p. 8) because it is based upon general geographic extents of geologic materials and not on an actual aquifer location as would be determined through a rigorous hydrogeologic investigation.

Probability of Elevated Volatile Organic Compound (VOC) Concentrations in Groundwater in the Eagle River Watershed Valley-Fill Aquifer, Eagle County, North-Central Colorado, 2006-2007

USGS Publications Warehouse

Rupert, Michael G.; Plummer, Niel

2009-01-01

This raster data set delineates the predicted probability of elevated volatile organic compound (VOC) concentrations in groundwater in the Eagle River watershed valley-fill aquifer, Eagle County, North-Central Colorado, 2006-2007. This data set was developed by a cooperative project between the U.S. Geological Survey, Eagle County, the Eagle River Water and Sanitation District, the Town of Eagle, the Town of Gypsum, and the Upper Eagle Regional Water Authority. This project was designed to evaluate potential land-development effects on groundwater and surface-water resources so that informed land-use and water management decisions can be made. This groundwater probability map and its associated probability maps was developed as follows: (1) A point data set of wells with groundwater quality and groundwater age data was overlaid with thematic layers of anthropogenic (related to human activities) and hydrogeologic data by using a geographic information system to assign each well values for depth to groundwater, distance to major streams and canals, distance to gypsum beds, precipitation, soils, and well depth. These data then were downloaded to a statistical software package for analysis by logistic regression. (2) Statistical models predicting the probability of elevated nitrate concentrations, the probability of unmixed young water (using chlorofluorocarbon-11 concentrations and tritium activities), and the probability of elevated volatile organic compound concentrations were developed using logistic regression techniques. (3) The statistical models were entered into a GIS and the probability map was constructed.

Probability of Elevated Nitrate Concentrations in Groundwater in the Eagle River Watershed Valley-Fill Aquifer, Eagle County, North-Central Colorado, 2006-2007

USGS Publications Warehouse

Rupert, Michael G.; Plummer, Niel

2009-01-01

This raster data set delineates the predicted probability of elevated nitrate concentrations in groundwater in the Eagle River watershed valley-fill aquifer, Eagle County, North-Central Colorado, 2006-2007. This data set was developed by a cooperative project between the U.S. Geological Survey, Eagle County, the Eagle River Water and Sanitation District, the Town of Eagle, the Town of Gypsum, and the Upper Eagle Regional Water Authority. This project was designed to evaluate potential land-development effects on groundwater and surface-water resources so that informed land-use and water management decisions can be made. This groundwater probability map and its associated probability maps was developed as follows: (1) A point data set of wells with groundwater quality and groundwater age data was overlaid with thematic layers of anthropogenic (related to human activities) and hydrogeologic data by using a geographic information system to assign each well values for depth to groundwater, distance to major streams and canals, distance to gypsum beds, precipitation, soils, and well depth. These data then were downloaded to a statistical software package for analysis by logistic regression. (2) Statistical models predicting the probability of elevated nitrate concentrations, the probability of unmixed young water (using chlorofluorocarbon-11 concentrations and tritium activities), and the probability of elevated volatile organic compound concentrations were developed using logistic regression techniques. (3) The statistical models were entered into a GIS and the probability map was constructed.

Mercury concentrations in tissues of Florida bald eagles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wood, P.B.; Wood, J.M.; White, J.H.

1996-01-01

We collected 48 blood and 61 feather samples from nestling bald eagles at 42 nests and adult feather samples from 20 nests in north and central Florida during 1991-93. We obtained 32 liver, 10 feather, and 5 blood samples from 33 eagle carcasses recovered in Florida during 1987-93. For nestlings, mercury concentrations in blood (GM = 0.16 ppm wet wt) and feather (GM = 3.23 ppm) samples were correlated (r = 0.69, P = 0.0001). Although nestlings had lower mercury concentrations in feathers than did adults (GM = 6.03 ppm), the feather mercury levels in nestlings and adults from themore » same nest were correlated (r = 0.63, P < 0.02). Mercury concentrations in blood of captive adult eagles (GM = 0.23 ppm) was similar to Florida nestlings but some Florida nestlings had blood mercury concentrations up to 0.61 ppm, more than twice as high as captive adults. Feather mercury concentrations in both nestlings and adults exceeded those in captive eagles, but concentrations in all tissues were similar to, or lower than, those in bald eagles from other wild populations. Although mercury concentrations in Florida eagles are below those that cause mortality, they are in the range of concentrations that can cause behavioral changes or reduce reproduction. We recommend periodic monitoring of mercury in Florida bald eagles for early detection of mercury increases before negative effects on reproduction occur. 26 refs., 5 figs., 2 tabs.« less

75 FR 74038 - Twin Eagle Resource Management, LLC; Supplemental Notice That Initial Market-Based Rate Filing...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-30

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER11-2154-000] Twin Eagle Resource Management, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for... proceeding, of Twin Eagle Resource Management, LLC's [[Page 74039

Assessment of exposure of fish to emerging contaminants in the Eagle Creek Watershed

EPA Science Inventory

The Eagle Creek Watershed (ECW) encompasses 162 square miles in central Indiana upstream of the Eagle Creek Reservoir, a public drinking water source for the city of Indianapolis. The dominant land-cover is agriculture, although some portions are undergoing urbanization, with th...

The Role of Pyruvate in Protecting 661W Photoreceptor-Like Cells Against Light-Induced Cell Death.

PubMed

Natoli, Riccardo; Rutar, Matt; Lu, Yen-Zhen; Chu-Tan, Joshua A; Chen, Yuwei; Saxena, Kartik; Madigan, Michele; Valter, Krisztina; Provis, Jan M

2016-11-01

Light is a requirement for the function of photoreceptors in visual processing. However, prolonged light exposure can be toxic to photoreceptors, leading to increased reactive oxygen species (ROS), lipid peroxidation, and photoreceptor cell death. We used the 661W mouse cone photoreceptor-like cell line to study the effects of pyruvate in protecting these cells from light-induced toxicity. 661W cells were exposed to 15,000 lux continuous bright light for 5 hours and incubated in Dulbecco's modified eagle medium (DMEM) with various concentrations of pyruvate. Following light damage, cells were assessed for changes in morphology, cell toxicity, viability, and ROS production. Mitochondrial respiration and anaerobic glycolysis were also assessed using a Seahorse Xfe96 extracellular flux analyzer. We found that cell death caused by light damage in 661W cells was dramatically reduced in the presence of pyruvate. Cells with pyruvate-supplemented media also showed attenuation of oxidative stress and maintained normal levels of ATP. We also found that alterations in the concentrations of pyruvate had no effect on mitochondrial respiration or glycolysis in light-damaged cells. Taken together, the results show that pyruvate is protective against light damage but does not alter the metabolic output of the cells, indicating an alternative role for pyruvate in reducing oxidative stress. Thus, sodium pyruvate is a possible candidate for the treatment against the oxidative stress component of retinal degenerations.

Cu-releasing bioactive glass/polycaprolactone coating on Mg with antibacterial and anticorrosive properties for bone tissue engineering.

PubMed

Yang, Yuyun; Zheng, Kai; Liang, Ruifang; Mainka, Astrid; Taccardi, Nicola; Roether, Judith A; Detsch, Rainer; Goldmann, Wolfgang H; Virtanen, Sannakaisa; Boccaccini, Aldo R

2017-10-26

Bioactive glass nanoparticles containing copper (Cu-BGNs) were introduced into polycaprolactone (PCL) coating systems to improve the bioactivity, antibacterial properties, and corrosion resistance of vulnerable magnesium matrices under physiological conditions. The influence of different amounts of Cu-BGNs in PCL coatings was thoroughly investigated in determining the wettability, electrochemical properties, and antibacterial effects against Staphylococcus carnosus and Escherichia coli, as well as their cyto-compatibility. Cu-BGNs were observed randomly scattered in PCL coatings. Increasing the concentration of Cu-BGNs resulted in a slight decrease of the water contact angle, and a reduction in anticorrosion properties of the Cu-BGN composite coatings. Yet higher Cu-BGN content in coatings led to more calcium phosphate formation on the surface after 7 days of immersion in Dulbecco's modified Eagle's medium, which was confirmed by Fourier-transform infrared spectroscopy and x-ray photoelectron spectroscopy. The growth of S. carnosus and E. coli was inhibited by Cu 2+ ions released from the Cu-BGN coatings. In addition, both direct and indirect cyto-compatibility experiments showed that the viability and proliferation of MG-63 cells on Cu-BGN coatings were highly increased compared to pure magnesium; however, an additional increase of Cu-BGN concentration showed a slight decrease of cell proliferation and cell activity. In summary, Cu-BGN/PCL composite coatings impart magnesium-based biomaterials with antibacterial and anticorrosive properties for clinical applications.

Leucine increases mucin 2 and occludin production in LS174T cells partially via PI3K-Akt-mTOR pathway.

PubMed

Mao, Xiangbing; Hu, Haiyan; Tang, Jun; Chen, Daiwen; Yu, Bing

2016-09-01

Mucin 2 and occludin play a crucial role in preserving the intestinal mucosal integrity. However, the role for leucine mediating intestinal mucin 2 and occludin expression has little been investigated. The current study was conducted to test the hypothesis that leucine treatment could increase mucin 2 and occludin levels in LS174T cells. The LS174T cells were incubated in the Dulbecco's Modified Eagle Medium (DMEM) supplementing 0, 0.5 and 5 mmol/L L-leucine for the various durations. Two hours after the leucine treatment, the inhibitor of mammalian target of rapamycin (mTOR) and protein kinase B (Akt) phosphorylation in LS174T cells were significantly increased ( P  < 0.05), and the mucin 2 and occludin levels were also significantly enhanced ( P  < 0.05). However, the pretreatment of 10 nmol/L rapamycin, which was an mTOR inhibitor, or 1 μmol/L wortmanin, which was an inhibitor of phosphatidylinositol 3-kinase (PI3K), completely inhibited leucine-induced mTOR or Akt phosphorylation ( P  < 0.05), and significantly reduced leucine-stimulated mucin 2 and occludin levels ( P  < 0.05). These results suggest that leucine treatment promotes the mucin 2 and occludin levels in LS174T cells partially through the PI3K-Akt-mTOR signaling pathway.

Isolation of circulating tumor cells by a magnesium-embedded filter

NASA Astrophysics Data System (ADS)

Liu, Yang; Xu, Tong; Xu, Yucheng; Kang, Dongyang; Xu, Lei; Park, Jungwook; Han-Chieh Chang, Jay; Zhang, Xiaoxiao; Goldkorn, Amir; Tai, Yu-Chong

2015-10-01

Circulating tumor cells (CTCs) are rare cancer cells that are shed by tumors into the bloodstream and that can be valuable biomarkers for various types of cancers. However, CTCs captured on the filter could not be released easily using the existing CTC analysis platforms based on size. To address this limitation, we have developed a novel magnesium (Mg)-embedded cell filter for capture, release and isolation of CTCs. The CTC-filter consists of a thin Ebeam-deposited Mg layer embedded between two parylene-C (PA-C) layers with designed slots for filtration and CTC capture. Thin Mg film has proved highly biocompatible and can be etched in saline, PBS and Dulbecco’s modified eagle medium (DMEM) etc, properties that are of great benefit to help dissociate the filter and thus release the cells. The finite element method (FEM) analysis was performed on the Mg etching process in DMEM for the structure design. After the filtration process, the filter was submerged in DMEM to facilitate Mg etching. The top PA-C filter pieces break apart from the bottom after Mg completely dissolves, enabling captured CTCs to detach. The released CTC can be easily aspirated into a micropipette for further analysis. Thus, the Mg-embedded cell filter provides a new and effective approach for CTCs isolation from the filter, making this a promising new strategy for cancer detection.

Albumin coatings by alternating current electrophoretic deposition for improving corrosion resistance and bioactivity of titanium implants.

PubMed

Höhn, Sarah; Braem, Annabel; Neirinck, Bram; Virtanen, Sannakaisa

2017-04-01

Although Ti alloys are generally regarded to be highly corrosion resistant, inflammatory conditions following surgery can instigate breakdown of the TiO 2 passivation layer leading to an increased metal ion release. Furthermore proteins present in the surrounding tissue will readily adsorb on a titanium surface after implantation. In this paper alternating current electrophoretic deposition (AC-EPD) of bovine serum albumin (BSA) on Ti6Al4V was investigated in order to increase the corrosion resistance and control the protein adsorption capability of the implant surface. The Ti6Al4V surface was characterized with SEM, XPS and ToF-SIMS after long-term immersion tests under physiological conditions and simulated inflammatory conditions either in Dulbecco's Modified Eagle Medium (DMEM) or DMEM supplemented with fetal calf serum (FCS). The analysis showed an increased adsorption of amino acids and proteins from the different immersion solutions. The BSA coating was shown to prevent selective dissolution of the vanadium (V) rich β-phase, thus effectively limiting metal ion release to the environment. Electrochemical impedance spectroscopy measurements confirmed an increase of the corrosion resistance for BSA coated surfaces as a function of immersion time due to the time-dependent adsorption of the different amino acids (from DMEM) and proteins (from FCS) as observed by ToF-SIMS analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

Infectivity of RNA from inactivated poliovirus.

PubMed

Nuanualsuwan, Suphachai; Cliver, Dean O

2003-03-01

During inactivation of poliovirus type 1 (PV-1) by exposure to UV, hypochlorite, and heat (72 degrees C), the infectivity of the virus was compared with that of its RNA. DEAE-dextran (1-mg/ml concentration in Dulbecco's modified Eagle medium buffered with 0.05 M Tris, pH 7.4) was used to facilitate transfecting PV-1 RNA into FRhK-4 host cells. After interaction of PV-1 RNA with cell monolayer at room temperature (21 to 22 degrees C) for 20 min, the monolayers were washed with 5 ml of Hanks balanced salt solution. The remainder of the procedure was the same as that for the conventional plaque technique, which was also used for quantifying the PV-1 whole-particle infectivity. Plaque formation by extracted RNA was approximately 100,000-fold less efficient than that by whole virions. The slopes of best-fit regression lines of inactivation curves for virion infectivity and RNA infectivity were compared to determine the target of inactivation. For UV and hypochlorite inactivation the slopes of inactivation curves of virion infectivity and RNA infectivity were not statistically different. However, the difference of slopes of inactivation curves of virion infectivity and RNA infectivity was statistically significant for thermal inactivation. The results of these experiments indicate that viral RNA is a primary target of UV and hypochlorite inactivations but that the sole target of thermal inactivation is the viral capsid.

Infectivity of RNA from Inactivated Poliovirus

PubMed Central

Nuanualsuwan, Suphachai; Cliver, Dean O.

2003-01-01

During inactivation of poliovirus type 1 (PV-1) by exposure to UV, hypochlorite, and heat (72°C), the infectivity of the virus was compared with that of its RNA. DEAE-dextran (1-mg/ml concentration in Dulbecco's modified Eagle medium buffered with 0.05 M Tris, pH 7.4) was used to facilitate transfecting PV-1 RNA into FRhK-4 host cells. After interaction of PV-1 RNA with cell monolayer at room temperature (21 to 22°C) for 20 min, the monolayers were washed with 5 ml of Hanks balanced salt solution. The remainder of the procedure was the same as that for the conventional plaque technique, which was also used for quantifying the PV-1 whole-particle infectivity. Plaque formation by extracted RNA was approximately 100,000-fold less efficient than that by whole virions. The slopes of best-fit regression lines of inactivation curves for virion infectivity and RNA infectivity were compared to determine the target of inactivation. For UV and hypochlorite inactivation the slopes of inactivation curves of virion infectivity and RNA infectivity were not statistically different. However, the difference of slopes of inactivation curves of virion infectivity and RNA infectivity was statistically significant for thermal inactivation. The results of these experiments indicate that viral RNA is a primary target of UV and hypochlorite inactivations but that the sole target of thermal inactivation is the viral capsid. PMID:12620852

Electrochemical detection of copper ions leached from CuO nanoparticles in saline buffers and biological media using a gold wire working electrode

NASA Astrophysics Data System (ADS)

Baldisserri, Carlo; Costa, Anna Luisa

2016-04-01

We performed explorative cyclic voltammetry in phosphate-buffered saline buffers, Dulbecco's modified Eagle's medium (DMEM), and fetal bovine serum-added DMEM using Au wire as working electrode, both in the absence and in the presence of known nominal concentrations of Cu2+ ions or 15 nm CuO nanoparticles. Addition of either Cu2+ ions or aqueous suspension of CuO nanoparticles caused a single anodic peak to appear in the double-layer region of all three pristine media. The height of the anodic peak was found to increase in a monotonic fashion vs. Cu2+ concentration in Cu2+-added media, and versus time since CuO addition in CuO-added media. Stepwise addition of glycine to Cu2+-added phosphate-buffered saline buffer caused an increasing cathodic shift of the anodic peak accompanied by decreasing peak currents. Results indicate that preparing Cu2+-free suspensions of CuO nanoparticles in such media is difficult, owing to the presence of leached copper ions. The implications on results of experiments in which CuO nanoparticle-added biological media are used as cell culture substrates are discussed. Literature data on the interactions between Cu2+ ions, dissolved carbon dioxide in aqueous CuO suspensions, and amino acids present in such media are compared to our results.

Alginate as a cell culture substrate for growth and differentiation of human retinal pigment epithelial cells.

PubMed

Heidari, Razeih; Soheili, Zahra-Soheila; Samiei, Shahram; Ahmadieh, Hamid; Davari, Maliheh; Nazemroaya, Fatemeh; Bagheri, Abouzar; Deezagi, Abdolkhalegh

2015-03-01

The purpose of this study was to evaluate retinal pigment epithelium (RPE) cells' behavior in alginate beads that establish 3D environment for cellular growth and mimic extracellular matrix versus the conventional 2D monolayer culture. RPE cells were encapsulated in alginate beads by dripping alginate cell suspension into CaCl2 solution. Beads were suspended in three different media including Dulbecco's modified Eagle's medium (DMEM)/F12 alone, DMEM/F12 supplemented with 10 % fetal bovine serum (FBS), and DMEM/F12 supplemented with 30 % human amniotic fluid (HAF). RPE cells were cultivated on polystyrene under the same conditions as controls. Cell phenotype, cell proliferation, cell death, and MTT assay, immunocytochemistry, and real-time RT-PCR were performed to evaluate the effect of alginate on RPE cells characteristics and integrity. RPE cells can survive and proliferate in alginate matrixes. Immunocytochemistry analysis exhibited Nestin, RPE65, and cytokeratin expressions in a reasonable number of cultured cells in alginate beads. Real-time PCR data demonstrated high levels of Nestin, CHX10, RPE65, and tyrosinase gene expressions in RPE cells immobilized in alginate when compared to 2D monolayer culture systems. The results suggest that alginate can be used as a reliable scaffold for maintenance of RPE cells' integrity and in vitro propagation of human retinal progenitor cells for cell replacement therapies in retinal diseases.

Experimental Calcium Silicate-Based Cement with and without Zirconium Oxide Modulates Fibroblasts Viability.

PubMed

Slompo, Camila; Peres-Buzalaf, Camila; Gasque, Kellen Cristina da Silva; Damante, Carla Andreotti; Ordinola-Zapata, Ronald; Duarte, Marco Antonio Hungaro; de Oliveira, Rodrigo Cardoso

2015-01-01

The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco's modified Eagle's medium (DMEM) for periods of 12, 24, and 48 h. Groups tested were white Portland cement (WPC), white Portland cement with zirconium oxide (WPCZO), and white mineral trioxide aggregate Angelus (MTA). Control group cells were not treated. The cytotoxicity was evaluated through mitochondrial-activity (MTT) and cell-density (crystal violet) assays. All cements showed low cytotoxicity. In general, at the concentration of 10 mg/mL there was an increase in viability of those groups treated with WPC and WPCZO when compared to the control group (p<0.05). A similar profile for the absorbance values was noted among the groups: 10 mg/mL presented an increase in viability compared to the control group. On the other hand, smaller concentrations presented a similar or lower viability compared to the control group, in general. A new dental material composed of calcium silicate-based cement with 20% zirconium oxide as the radiopacifier showed low cytotoxicity as a promising material to be exploited for root-end filling.

Establishment and characterization of a brain cell line from sea perch, Lateolabrax japonicus.

PubMed

Le, Yao; Li, Yunlong; Jin, Yilin; Jia, Peng; Jia, Kuntong; Yi, Meisheng

2017-10-01

A continuous cell line, designated LJB, derived from the brain of sea perch (Lateolabrax japonicus) was established. LJB cells have been subcultured for more than 60 times in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum (FBS) since the initial primary culture. LJB cells exhibited maximum growth rate at 28°C in DMEM supplemented with 20% FBS. Cytogenetic analysis indicated that the modal chromosome number was 48, which was identical with the chromosome number of embryonic stem-like cells of sea perch. Comparison of the 18S ribosomal RNA gene sequences of LJB cells and sea perch confirmed that LJB cells originated from sea perch. After transfected with pEGFP-N3 plasmid, LJB cells showed a transfection efficiency of about 40% which was indicated by the percentage of cells expressing green fluorescence protein, indicating the potential application of LJB cells in gene expression studies. Cytopathic effect was clearly observed, and RNA-dependent RNA polymerase gene was also detected in LJB cells post red-spotted grouper nervous necrosis virus (RGNNV) infection. Furthermore, virus replication was confirmed by quantitative RT-PCR, virus titer, and transmission electron microscopy assay in RGNNV-infected LJB cells. The LJB cell line might be used as an ideal in vitro tool for analyzing and understanding the mechanisms of nervous necrosis virus-host interaction.

In vitro activities of plant extracts on human Loa loa isolates and cytotoxicity for eukaryotic cells.

PubMed

Mengome, Line-Edwige; Akue, Jean Paul; Souza, Alain; Feuya Tchoua, Guy Raymond; Nsi Emvo, Edouard

2010-08-01

Loa loa, a filarial worm, can cause fatal encephalitis in humans. In an attempt to find alternatives to the standard treatments (ivermectin and diethylcarbamazine citrate), we tested 12 methanolic extracts of nine traditional plant remedies. The extracts (100-0.09 microg/ml) were incubated with 20 Loa loa microfilariae isolated from patients at 37 degrees C with 5% CO(2) in modified Eagle's medium supplemented with 10% fetal serum and antibiotics. Activity was evaluated 120 h later by counting live microfilariae under a microscope. Cytotoxicity for eukaryotic cells was estimated by measuring 3-[4,5-dimethylthiazol-2-yl]-2-5 diphenyl tetrazolium bromide transformation to formazan at 450 nM in a spectrophotometer. The plants tested were Lophira alata, Greenwayodendron suaveolens, Uapaca togoensis, Zanthoxylum heitzii, Peperomia pellucida, Piptadeniastrum africanum, Petersianthus macrocarpus, Vernonia conferta, and Vernonia hymenolepis. Chemical screening showed that most of the extracts contained reducing sugars, tannin or polyphenols, sterols or triterpenes, saponosides, and alkaloids. None contained carotinoids and few contained flavonoids. The 50% lethal concentration ranged from 0.22 to 70.28 microg/ml, while the 50% inhibitory concentration for eukaryotic cells (IC(50)) ranged from 8.52 to 119.52 microg/ml. Extracts of P. macrocarpus (selectivity index = 72.16), P. africanum (13.69), Z. heitzii (12.11), and L. alata (9.26) were highly selective for L. loa.

Optimisation of isolation of richly pure and homogeneous primary human colonic smooth muscle cells.

PubMed

Tattoli, I; Corleto, V D; Taffuri, M; Campanini, N; Rindi, G; Caprilli, R; Delle Fave, G; Severi, C

2004-11-01

Inherent properties of gastrointestinal smooth muscle can be assessed using isolated cell suspensions. Currently available isolation techniques, based on short 2-h enzymatic digestion, however, present the disadvantage of low cellular yield with brief viability. These features are an important limiting factor especially in studies in humans in which tissue may not be available daily and mixing of samples is not recommended. To optimise the isolation procedure of cells from human colon to obtain a richly pure primary smooth muscle cell preparation. Slices of circular muscle layer, obtained from surgical specimens of human colon, were incubated overnight in Dulbecco's modified eagle's medium supplemented with antibiotics, foetal bovine serum, an ATP-regenerating system and collagenase. On the following day, digested muscle strips were suspended in HEPES buffer, and spontaneously dissociated smooth muscle cells were harvested and used either immediately or maintained in suspension for up to 72 h. Cell yield, purity, viability, contractile responses, associated intracellular calcium signals and RNA and protein extraction were evaluated and compared to cell suspensions obtained with the current short digestion protocol. The overnight isolation protocol offers the advantage of obtaining a pure, homogeneous, long-life viable cell suspension that maintains a fully differentiated smooth muscle phenotype unchanged for at least 72 h and that allows multiple functional/biochemical studies and efficient RNA extraction from a single human specimen.

Factors Ruling the Uptake of Silica Nanoparticles by Mesenchymal Stem Cells: Agglomeration Versus Dispersions, Absence Versus Presence of Serum Proteins.

PubMed

Catalano, Federico; Accomasso, Lisa; Alberto, Gabriele; Gallina, Clara; Raimondo, Stefania; Geuna, Stefano; Giachino, Claudia; Martra, Gianmario

2015-06-24

The results of a systematic investigation of the role of serum proteins on the interaction of silica nanoparticles (NP) doped in their bulk with fluorescent molecules (IRIS Dots, 50 nm in size), with human mesenchymal stem cells (hMSCs) are reported. The suspension of IRIS Dots in bare Dulbecco-modified Eagle's medium results in the formation of large agglomerates (≈1.5 μm, by dynamic light scattering), which become progressively smaller, down to ≈300 nm in size, by progressively increasing the fetal bovine serum (FBS) content of the solutions along the series 1.0%, 2.5%, 6.0%, and 10.0% v/v. Such difference in NP dispersion is maintained in the external cellular microenvironment, as observed by confocal microscopy and transmission electron microscopy. As a consequence of the limited diffusion of proteins in the inter-NP spaces, the surface of NP agglomerates is coated by a protein corona independently of the agglomerate size/FBS concentration conditions (ζ-potential and UV circular dichroism measurements). The protein corona appears not to be particularly relevant for the uptake of IRIS Dots by hMSCs, whereas the main role in determining the internalization rate is played by the absence/presence of serum proteins in the extracellular media. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A serum factor that recalcifies demineralized bone is conserved in bony fish and sharks but is not found in invertebrates.

PubMed

Hamlin, N J; Ong, K G; Price, P A

2006-05-01

We investigated the evolutionary origin of a serum activity that induces calcification within a type I collagen matrix, an activity previously described in rat and bovine serum. Serum was obtained from vertebrates with calcified tissues (bony fish and shark), vertebrates without calcified tissues (lamprey and hagfish), and three invertebrates (marine worm, crab, and sea urchin). Serum from the bony fish and shark proved to contain a potent nucleator of collagen calcification; like the previously described calcifying activity in rat serum, the fish and shark activities are both able to recalcify a demineralized rat tibia when tested in Dulbecco's modified Eagle medium containing as little as 1.5% of the respective serum and have an apparent molecular weight of 50-150 kDa. No calcifying activity could be detected in any of several experimental tests of invertebrate or hagfish serum. Weak calcifying activity could be detected in lamprey serum, but calcification was restricted to the growth plate of the decalcified tibia, with no detectable calcification in the type I collagen of the midshaft. These studies reveal a correlation between the evolutionary timing of the appearance of calcified tissues in vertebrates and the appearance of the serum activity that initiates calcification within collagen and, therefore, support the hypothesis that this serum activity may play a role in normal calcification of bone.

75 FR 34530 - Nittany & Bald Eagle Railroad Company-Temporary Trackage Rights Exemption-Norfolk Southern...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-17

... Eagle Railroad Company--Temporary Trackage Rights Exemption--Norfolk Southern Railway Company Norfolk Southern Railway Company (NSR), pursuant to a written trackage rights agreement dated April 13, 2010, has agreed to grant nonexclusive overhead temporary trackage rights to Nittany & Bald Eagle Railroad Company...

77 FR 18881 - Nittany & Bald Eagle Railroad Company-Temporary Trackage Rights Exemption-Norfolk Southern...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-28

... Eagle Railroad Company--Temporary Trackage Rights Exemption--Norfolk Southern Railway Company Norfolk Southern Railway Company (NSR), pursuant to a written trackage rights agreement dated February 3, 2012, has agreed to grant nonexclusive overhead temporary trackage rights to Nittany & Bald Eagle Railroad Company...

75 FR 62184 - Notification of United States Mint Silver Eagle Bullion Coin Premium Increase

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-07

... Bullion Coin Premium Increase ACTION: Notification of United States Mint Silver Eagle Bullion Coin Premium Increase. SUMMARY: The United States Mint is increasing the premium charged to Authorized Purchasers for... will increase the premium charged to Authorized Purchasers for American Eagle Silver Bullion Coins...

77 FR 5505 - Eagle Crest Energy Company; Notice of Availability of the Final Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-03

... Energy Projects has reviewed the application for license for the Eagle Mountain Pumped Storage... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13123-002--California] Eagle Crest Energy Company; Notice of Availability of the Final Environmental Impact Statement for the...

76 FR 1149 - Eagle Crest Energy Company; Notice of Availability of the Draft Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-07

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13123-002--California] Eagle Crest Energy Company; Notice of Availability of the Draft Environmental Impact Statement for the Eagle Mountain Pumped Storage Hydroelectric Project and Notice of Public Meetings December 23, 2010. In...

50 CFR 22.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... remaining take is unavoidable. Area nesting population means the number of pairs of golden eagles known to.... Communal roost site means an area where eagles gather repeatedly in the course of a season and shelter.... Foraging area means an area where eagles regularly feed during one or more seasons. Import for the purpose...

50 CFR 22.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... remaining take is unavoidable. Area nesting population means the number of pairs of golden eagles known to.... Communal roost site means an area where eagles gather repeatedly in the course of a season and shelter.... Foraging area means an area where eagles regularly feed during one or more seasons. Import for the purpose...

50 CFR 22.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... remaining take is unavoidable. Area nesting population means the number of pairs of golden eagles known to.... Communal roost site means an area where eagles gather repeatedly in the course of a season and shelter.... Foraging area means an area where eagles regularly feed during one or more seasons. Import for the purpose...

77 FR 15395 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-Cooperative...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-15

... Production Act of 1993--Cooperative Research Group on Mechanical Stratigraphy and Natural Deformation in... Research Group on Mechanical Stratigraphy and Natural Deformation in Eagle Ford Formation and Equivalent... Eagle Ford's planned activity is to understand mechanical stratigraphy and natural deformation in Eagle...

77 FR 22267 - Eagle Permits; Changes in the Regulations Governing Eagle Permitting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-13

... with rotating wind turbines. Permit Duration and Transferability In February 2011, we published draft... permit applicants, because of the known risk to eagles from collisions with wind turbines and electric..., shoot at, poison, wound, kill, capture, trap, collect, destroy, molest, or disturb'' (Sec. 22.3). The...

77 FR 839 - Pricing for 2011 American Eagle Silver Uncirculated Coins

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-06

... DEPARTMENT OF THE TREASURY United States Mint Pricing for 2011 American Eagle Silver Uncirculated Coins Agency: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the re-pricing of the 2011 American Eagle Silver Uncirculated Coins. The price of...

76 FR 27182 - Pricing for American Eagle and American Buffalo Bullion Presentation Cases

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-10

... DEPARTMENT OF THE TREASURY United States Mint Pricing for American Eagle and American Buffalo Bullion Presentation Cases AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the price increase of the American Eagle/Buffalo Bullion...

76 FR 53717 - Pricing for the 2011 American Eagle Silver Uncirculated Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-29

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the 2011 American Eagle Silver Uncirculated Coin AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the pricing of the 2011 American Eagle Silver Uncirculated Coin. The price of...

76 FR 27753 - American Eagle Savings Bank, Boothwyn, PA; Approval of Conversion Application

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-12

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [AC-61: OTS Nos. 07212] American Eagle Savings Bank, Boothwyn, PA; Approval of Conversion Application Notice is hereby given that on May 3, 2011, the Office of Thrift Supervision approved the application of American Eagle Savings Bank, Boothwyn...

ASBO Eagle Institute: A Leadership Opportunity

ERIC Educational Resources Information Center

Scharff, James

2012-01-01

Each summer, ASBO International conducts an Eagle Institute leadership session in the Washington, D.C., area that provides a group of about 25 participants, including Eagle Award recipients, an opportunity to network with and learn from exemplary leaders inside and outside the field of school business management. Each year, the focus of the…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fratanduono, M.

Garcia and Associates (GANDA) was contracted by the Lawrence Livermore National Laboratory (LLNL) to collect information on golden eagle (Aquila chrysaetos) use of Site 300. During 2014, we conducted surveys at Site 300 and for an area including a 10-mile radius of Site 300. Those surveys documented 42 golden eagle territories including two territories that overlapped with Site 300. These were named ‘Tesla’ and ‘Linac Road’. In 2015, we conducted surveys to refine the territory boundaries of golden eagle territories that overlapped with Site 300 and to document eagle activity at Site 300.

Predatory threat of harpy eagles for yellow-breasted capuchin monkeys in the Atlantic Forest.

PubMed

Suscke, Priscila; Verderane, Michele; de Oliveira, Robson Santos; Delval, Irene; Fernández-Bolaños, Marcelo; Izar, Patrícia

2017-01-01

We describe seven encounters between different harpy eagle individuals (Harpia harpyja) and a group of yellow-breasted capuchin monkeys (Sapajus xanthosternos) in Una Biological Reserve. These interactions lasted 58 min on average. In each of those encounters, the capuchin monkeys used particular behavioral strategies against the harpy eagle that were not employed in reaction to other aerial predators. We did not observe any successful predation events, but after one of those encounters an infant disappeared from the capuchin group. As a whole, these observations indicate that the presence of harpy eagles in the group's home range increases predation risk for capuchin monkeys. The present report also suggests a reoccupation by H. harpyja of this area, as no previous recent records identify harpy eagle occurrence in Una Biological Reserve.

Ancient DNA provides new insights into the evolutionary history of New Zealand's extinct giant eagle.

PubMed

Bunce, Michael; Szulkin, Marta; Lerner, Heather R L; Barnes, Ian; Shapiro, Beth; Cooper, Alan; Holdaway, Richard N

2005-01-01

Prior to human settlement 700 years ago New Zealand had no terrestrial mammals--apart from three species of bats--instead, approximately 250 avian species dominated the ecosystem. At the top of the food chain was the extinct Haast's eagle, Harpagornis moorei. H. moorei (10-15 kg; 2-3 m wingspan) was 30%-40% heavier than the largest extant eagle (the harpy eagle, Harpia harpyja), and hunted moa up to 15 times its weight. In a dramatic example of morphological plasticity and rapid size increase, we show that the H. moorei was very closely related to one of the world's smallest extant eagles, which is one-tenth its mass. This spectacular evolutionary change illustrates the potential speed of size alteration within lineages of vertebrates, especially in island ecosystems.

Common Raven (Corvus corax) kleptoparasitism at a Golden Eagle (Aquila chyrsaetos) nest in southern Nevada

USGS Publications Warehouse

Simes, Matthew; Johnson, Diego R.; Streit, Justin; Longshore, Kathleen M.; Nussear, Kenneth E.; Esque, Todd C.

2017-01-01

The Common Raven (Corvus corax) is a ubiquitous species in the Mojave Desert of southern Nevada and California. From 5 to 24 May 2014, using remote trail cameras, we observed ravens repeatedly kleptoparasitizing food resources from the nest of a pair of Golden Eagles (Aquila chyrsaetos) in the Spring Mountains of southern Nevada. The ravens fed on nine (30%) of the 30 prey items delivered to the nest during the chick rearing period. Kleptoparasitic behavior by the ravens decreased as the eagle nestling matured to seven weeks of age, suggesting a narrow temporal window in which ravens can successfully engage in kleptoparasitic behavior at eagle nests. The observation of kleptoparasitism by Common Ravens at the nest suggests potential risks to young Golden Eagles from Common Ravens.

Correlates of immune defenses in golden eagle nestlings

USGS Publications Warehouse

MacColl, Elisabeth; Vanesky, Kris; Buck, Jeremy A.; Dudek, Benjamin; Eagles-Smith, Collin A.; Heath, Julie A.; Herring, Garth; Vennum, Chris; Downs, Cynthia J.

2017-01-01

An individual's investment in constitutive immune defenses depends on both intrinsic and extrinsic factors. We examined how Leucocytozoon parasite presence, body condition (scaled mass), heterophil-to-lymphocyte (H:L) ratio, sex, and age affected immune defenses in golden eagle (Aquila chrysaetos) nestlings from three regions: California, Oregon, and Idaho. We quantified hemolytic-complement activity and bacterial killing ability, two measures of constitutive immunity. Body condition and age did not affect immune defenses. However, eagles with lower H:L ratios had lower complement activity, corroborating other findings that animals in better condition sometimes invest less in constitutive immunity. In addition, eagles with Leucocytozoon infections had higher concentrations of circulating complement proteins but not elevated opsonizing proteins for all microbes, and eagles from Oregon had significantly higher constitutive immunity than those from California or Idaho. We posit that Oregon eagles might have elevated immune defenses because they are exposed to more endoparasites than eagles from California or Idaho, and our results confirmed that the OR region has the highest rate of Leucocytozoon infections. Our study examined immune function in a free-living, long-lived raptor species, whereas most avian ecoimmunological research focuses on passerines. Thus, our research informs a broad perspective regarding the evolutionary and environmental pressures on immune function in birds.

Migration of Florida sub-adult Bald Eagles

USGS Publications Warehouse

Mojica, E.K.; Meyers, J.M.; Millsap, B.A.; Haley, K.L.

2008-01-01

We used satellite telemetry locations accurate within 1 km to identify migration routes and stopover sites of 54 migratory sub-adult Bald Eagles (Haliaeetus leucocephalus) hatched in Florida from 1997 to 2001. We measured number of days traveled during migration, path of migration, stopover time and locations, and distance traveled to and from winter and summer areas for each eagle (1?5 years old). Eagles used both Coastal Plain (n = 24) and Appalachian Mountain (n = 26) routes on their first migration north. Mountain migrants traveled farther (X = 2,112 km; 95% CI: 1,815-2,410) than coastal migrants (X = 1,397 km; 95% CI: 1,087?1,706). Eagles changed between migration routes less often on northbound and southbound movements as they matured (X2 = 13.22, df = 2, P < 0.001). One-year-old eagles changed routes between yearly spring and fall migrations 57% of the time, 2-year-olds 30%, and 3-5-year-olds changed only 17% of the time. About half (n = 25, 46%) used stopovers during migration and stayed 6-31 days (X = 14.8 days; 95% CI: 12.8-16.8). We recommend that migratory stopover site locations be added to GIS data bases for improving conservation of Bald Eagles in the eastern United States.

Sagan Lecture: Spirit, Opportunity, and the Exploration of the Red Planet

NASA Astrophysics Data System (ADS)

Squyres, S.

2005-12-01

In January of 2004, twin robotic explorers named Spirit and Opportunity landed on Mars. Expected to last for 90 days, the two rovers have now been exploring the martian surface for more than a year and a half. Their objective is to search for evidence of past water on Mars, and to determine if Mars ever had conditions that would have been suitable for life. Spirit landed in Gusev Crater, a large impact crater in the southern highlands of Mars. Finding only basaltic lava on the crater floor, Spirit drove almost three kilometers to the base of the Columbia Hills, a small mountain range to the east of the landing site. There Spirit has ascended Husband Hill, the highest summit in the range, and has found strong evidence that the rocks there were modified long ago by water. Opportunity landed on Meridiani Planum, a smooth plateau near the martian equator, coming to rest in a small impact feature named Eagle Crater. Within Eagle Crater, Opportunity found compelling evidence for long-ago water on Mars. This evidence included 'blueberries': small concretions rich in hematite that precipitated from liquid water. It also included rocks that are made largely of sulfate salts, deposited when water evaporated away, and rocks that preserve ancient ripples that formed billions of years ago as water flowed over sand on Mars. The conditions long ago at Eagle Crater may have been suitable for some simple forms of life. Whether life could have developed there, however, is a much more difficult question. After leaving Eagle Crater, Opportunity drove eastward to Endurance Crater, a much larger crater that allowed access to deeper and older rocks which also proved to be blueberry-laden sulfate-rich sediments. Since leaving Endurance Crater, Opportunity has explored southward, driving more than five kilometers across the martian surface. The talk will provide an up-to-date summary of the mission of Spirit and Opportunity, from their initial conception through their launch, landing, and operations on the surface of Mars.

Pseudoscalar D and B mesons in the hot dense and nonstrange symmetric medium

NASA Astrophysics Data System (ADS)

Chhabra, Rahul; Kumar, Arvind

2017-01-01

We investigate the effect of temperature and density on the shift in the masses and decay constants of the pseudoscalar D and B mesons in the nonstrange symmetric medium. We use chiral SU(3) model to calculate the medium modified scalar and isoscalar fields σ, ζ, δ and χ. We use these modified fields to calculate the in-medium quark and gluon condensates by solving the coupled equations of motions in the chiral SU(3) model. We obtain the medium modified mass and decay constant through these medium modified condensates using the QCD sum rules. Further we use the 3P0 model by taking the internal structure of the mesons to calculate the in-medium decay width of the higher charmonium states χ(3556) , ψ(3686) and ψ(3770) to the D D pairs, through the in-medium mass of D meson and neglecting the mass modification of higher charmonium states. We also compare the present data with the previous results. These results of present investigation may be important to explain the possible outcomes of the experiments like CBM, Panda at GSI.

Increased Flight Altitudes among Migrating Golden Eagles Suggest Turbine Avoidance at a Rocky Mountain Wind Installation

PubMed Central

Johnston, Naira N.; Bradley, James E.; Otter, Ken A.

2014-01-01

Potential wind-energy development in the eastern Rocky Mountain foothills of British Columbia, Canada, raises concerns due to its overlap with a golden eagle (Aquila chrysaetos) migration corridor. The Dokie 1 Wind Energy Project is the first development in this area and stands as a model for other projects in the area because of regional consistency in topographic orientation and weather patterns. We visually tracked golden eagles over three fall migration seasons (2009–2011), one pre- and two post-construction, to document eagle flight behaviour in relation to a ridge-top wind energy development. We estimated three-dimensional positions of eagles in space as they migrated through our study site. Flight tracks were then incorporated into GIS to ascertain flight altitudes for eagles that flew over the ridge-top area (or turbine string). Individual flight paths were designated to a category of collision-risk based on flight altitude (e.g. flights within rotor-swept height; ≤150 m above ground) and wind speed (winds sufficient for the spinning of turbines; >6.8 km/h at ground level). Eagles were less likely to fly over the ridge-top area within rotor-swept height (risk zone) as wind speed increased, but were more likely to make such crosses under headwinds and tailwinds compared to western crosswinds. Most importantly, we observed a smaller proportion of flights within the risk zone at wind speeds sufficient for the spinning of turbines (higher-risk flights) during post-construction compared to pre-construction, suggesting that eagles showed detection and avoidance of turbines during migration. PMID:24671199

Minimising mortality in endangered raptors due to power lines: the importance of spatial aggregation to optimize the application of mitigation measures.

PubMed

Guil, Francisco; Fernández-Olalla, Mariana; Moreno-Opo, Rubén; Mosqueda, Ignacio; Gómez, María Elena; Aranda, Antonio; Arredondo, Angel; Guzmán, José; Oria, Javier; González, Luis Mariano; Margalida, Antoni

2011-01-01

Electrocution by power lines is one of the main causes of non-natural mortality in birds of prey. In an area in central Spain, we surveyed 6304 pylons from 333 power lines to determine electrocution rates, environmental and design factors that may influence electrocution and the efficacy of mitigation measures used to minimise electrocution cases. A total of 952 electrocuted raptors, representing 14 different species, were observed. Electrocuted raptors were concentrated in certain areas and the environmental factors associated with increased electrocution events were: greater numbers of prey animals; greater vegetation cover; and shorter distance to roads. The structural elements associated with electrocutions were shorter strings of insulators, one or more phases over the crossarm, cross-shaped design and pylon function. Of the 952 carcasses found, 148 were eagles, including golden eagle (Aquila chrysaetos), Spanish imperial eagle (Aquila adalberti) and Bonelli's eagle (Aquila fasciata). Electrocuted eagles were clustered in smaller areas than other electrocuted raptors. The factors associated with increased eagle electrocution events were: pylons function, shorter strings of insulators, higher slopes surrounding the pylon, and more numerous potential prey animals. Pylons with increased string of insulators had lower raptor electrocution rates than unimproved pylons, although this technique was unsuccessful for eagles. Pylons with cable insulation showed higher electrocution rates than unimproved pylons, both for raptors and eagles, despite this is the most widely used and recommended mitigation measure in several countries. To optimize the application of mitigation measures, our results recommend the substitution of pin-type insulators to suspended ones and elongating the strings of insulators.

Probability of Unmixed Young Groundwater (defined using chlorofluorocarbon-11 concentrations and tritium activities) in the Eagle River Watershed Valley-Fill Aquifer, Eagle County, North-Central Colorado, 2006-2007

USGS Publications Warehouse

Rupert, Michael G.; Plummer, Niel

2009-01-01

This raster data set delineates the predicted probability of unmixed young groundwater (defined using chlorofluorocarbon-11 concentrations and tritium activities) in groundwater in the Eagle River watershed valley-fill aquifer, Eagle County, North-Central Colorado, 2006-2007. This data set was developed by a cooperative project between the U.S. Geological Survey, Eagle County, the Eagle River Water and Sanitation District, the Town of Eagle, the Town of Gypsum, and the Upper Eagle Regional Water Authority. This project was designed to evaluate potential land-development effects on groundwater and surface-water resources so that informed land-use and water management decisions can be made. This groundwater probability map and its associated probability maps were developed as follows: (1) A point data set of wells with groundwater quality and groundwater age data was overlaid with thematic layers of anthropogenic (related to human activities) and hydrogeologic data by using a geographic information system to assign each well values for depth to groundwater, distance to major streams and canals, distance to gypsum beds, precipitation, soils, and well depth. These data then were downloaded to a statistical software package for analysis by logistic regression. (2) Statistical models predicting the probability of elevated nitrate concentrations, the probability of unmixed young water (using chlorofluorocarbon-11 concentrations and tritium activities), and the probability of elevated volatile organic compound concentrations were developed using logistic regression techniques. (3) The statistical models were entered into a GIS and the probability map was constructed.

Increased flight altitudes among migrating golden eagles suggest turbine avoidance at a Rocky Mountain wind installation.

PubMed

Johnston, Naira N; Bradley, James E; Otter, Ken A

2014-01-01

Potential wind-energy development in the eastern Rocky Mountain foothills of British Columbia, Canada, raises concerns due to its overlap with a golden eagle (Aquila chrysaetos) migration corridor. The Dokie 1 Wind Energy Project is the first development in this area and stands as a model for other projects in the area because of regional consistency in topographic orientation and weather patterns. We visually tracked golden eagles over three fall migration seasons (2009-2011), one pre- and two post-construction, to document eagle flight behaviour in relation to a ridge-top wind energy development. We estimated three-dimensional positions of eagles in space as they migrated through our study site. Flight tracks were then incorporated into GIS to ascertain flight altitudes for eagles that flew over the ridge-top area (or turbine string). Individual flight paths were designated to a category of collision-risk based on flight altitude (e.g. flights within rotor-swept height; ≤150 m above ground) and wind speed (winds sufficient for the spinning of turbines; >6.8 km/h at ground level). Eagles were less likely to fly over the ridge-top area within rotor-swept height (risk zone) as wind speed increased, but were more likely to make such crosses under headwinds and tailwinds compared to western crosswinds. Most importantly, we observed a smaller proportion of flights within the risk zone at wind speeds sufficient for the spinning of turbines (higher-risk flights) during post-construction compared to pre-construction, suggesting that eagles showed detection and avoidance of turbines during migration.

Evaluation of Cariogenic Bacteria

PubMed Central

Nishikawara, Fusao; Nomura, Yoshiaki; Imai, Susumu; Senda, Akira; Hanada, Nobuhiro

2007-01-01

Objectives The evaluation of Mutans streptococci (MS) is one of the index for caries risk. DentocultTM and CRTTM are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar. Methods The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA. Results The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRTTM, (P< .01) and Dentocult SMTM (P<.05). Conclusion The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor. PMID:19212495

75 FR 11937 - Eagle Sportswear, Inc.; New York, NY; Notice of Termination of Investigation

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-73,023] Eagle Sportswear, Inc.; New York, NY; Notice of Termination of Investigation Pursuant to Section 223 of the Trade Act of 1974... company official on behalf of workers of Eagle Sportswear, Inc., New York, New York. The petitioner has...

Publications - PIR 2002-1A | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2002-1A Publication Details Title: Geologic map of the Eagle A-1 Quadrangle, Fortymile publication sales page for more information. Quadrangle(s): Eagle Bibliographic Reference Szumigala, D.J ., 2002, Geologic map of the Eagle A-1 Quadrangle, Fortymile mining district: Alaska Division of

77 FR 40704 - Price for the 2012 American Eagle Silver Uncirculated Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... DEPARTMENT OF THE TREASURY United States Mint Price for the 2012 American Eagle Silver... States Mint is announcing the price of the 2012 American Eagle Silver Uncirculated Coin. The coin will be offered for sale at a price of $45.95. FOR FURTHER INFORMATION CONTACT: B. B. Craig, Associate Director...

76 FR 20971 - Eagle Rock Desoto Pipeline, L.P.; Notice of Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-14

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-120-001] Eagle Rock Desoto Pipeline, L.P.; Notice of Filing Take notice that on April 7, 2011, Eagle Rock Desoto Pipeline, L.P. filed a revised Statement of Operating Conditions to comply with an unpublished delegated letter...

76 FR 67799 - Pricing for the American Eagle 25th Anniversary Silver Coin Set

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-02

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the American Eagle 25th Anniversary Silver Coin Set AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the price of the American Eagle 25th Anniversary Silver Coin Set. The...

77 FR 15457 - Pricing for the 2012 American Eagle Silver Proof Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-15

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the 2012 American Eagle Silver Proof Coin AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the price of the 2012 American Eagle Silver Proof Coin. The coins will be offered...

76 FR 65563 - Pricing for 2011 American Eagle Silver Proof and Uncirculated Coins

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... DEPARTMENT OF THE TREASURY United States Mint Pricing for 2011 American Eagle Silver Proof and Uncirculated Coins AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the re-pricing of the 2011 American Eagle Silver Proof and Uncirculated Coins...

76 FR 53717 - Pricing for the 2011 American Eagle Silver Proof Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-29

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the 2011 American Eagle Silver Proof Coin AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the re-pricing of the 2011 American Eagle Silver Proof Coin. The price of the coins...

76 FR 33026 - Pricing for the 2011 American Eagle Silver Proof Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-07

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the 2011 American Eagle Silver Proof Coin AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the price of the 2011 American Eagle Silver Proof Coin. The coin will be offered for...

50 CFR 22.26 - Permits for eagle take that is associated with, but not the purpose of, an activity.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Other interests. (5) Any additional factors that may be relevant to our decision whether to issue the..., SALE, PURCHASE, BARTER, EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) EAGLE PERMITS... cumulative effects of other permitted take and other additional factors affecting eagle populations; (ii...

77 FR 25164 - Adequacy Status of the Eagle River, Alaska Particulate Matter Limited Maintenance Plan for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

... ENVIRONMENTAL PROTECTION AGENCY [EPA-R10-OAR-2010-1914; FRL-9664-7] Adequacy Status of the Eagle River, Alaska Particulate Matter Limited Maintenance Plan for Transportation Conformity Purposes AGENCY... notifying the public of its finding that the Eagle River, Alaska, Particulate Matter (PM 10 ) Limited...

Nature Photography - Bald Eagle

NASA Image and Video Library

2016-12-12

An American bald eagle perches in a tree at NASA's Kennedy Space Center in Florida. Several eagles call the center home. The center shares a boundary with the Merritt Island National Wildlife Refuge. The refuge is home to more than 65 amphibian and reptile species, along with 330 native and migratory bird species, 25 mammal and 117 fish species.

76 FR 387 - Atomic Safety and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-04

... and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility) December 17, 2010... construction and operation of a gas centrifuge uranium enrichment facility--denoted as the Eagle Rock... site at http://www.nrc.gov/materials/fuel-cycle-fac/arevanc.html . These and other documents relating...

Ancient DNA Provides New Insights into the Evolutionary History of New Zealand's Extinct Giant Eagle

PubMed Central

Szulkin, Marta; Lerner, Heather R. L; Barnes, Ian; Shapiro, Beth; Cooper, Alan; Holdaway, Richard N

2005-01-01

Prior to human settlement 700 years ago New Zealand had no terrestrial mammals—apart from three species of bats—instead, approximately 250 avian species dominated the ecosystem. At the top of the food chain was the extinct Haast's eagle, Harpagornis moorei. H. moorei (10–15 kg; 2–3 m wingspan) was 30%–40% heavier than the largest extant eagle (the harpy eagle, Harpia harpyja), and hunted moa up to 15 times its weight. In a dramatic example of morphological plasticity and rapid size increase, we show that the H. moorei was very closely related to one of the world's smallest extant eagles, which is one-tenth its mass. This spectacular evolutionary change illustrates the potential speed of size alteration within lineages of vertebrates, especially in island ecosystems. PMID:15660162

Adaptive EAGLE dynamic solution adaptation and grid quality enhancement

NASA Technical Reports Server (NTRS)

Luong, Phu Vinh; Thompson, J. F.; Gatlin, B.; Mastin, C. W.; Kim, H. J.

1992-01-01

In the effort described here, the elliptic grid generation procedure in the EAGLE grid code was separated from the main code into a subroutine, and a new subroutine which evaluates several grid quality measures at each grid point was added. The elliptic grid routine can now be called, either by a computational fluid dynamics (CFD) code to generate a new adaptive grid based on flow variables and quality measures through multiple adaptation, or by the EAGLE main code to generate a grid based on quality measure variables through static adaptation. Arrays of flow variables can be read into the EAGLE grid code for use in static adaptation as well. These major changes in the EAGLE adaptive grid system make it easier to convert any CFD code that operates on a block-structured grid (or single-block grid) into a multiple adaptive code.

EAGLE Monitors by Collecting Facts and Generating Obligations

NASA Technical Reports Server (NTRS)

Barrnger, Howard; Goldberg, Allen; Havelund, Klaus; Sen, Koushik

2003-01-01

We present a rule-based framework, called EAGLE, that has been shown to be capable of defining and implementing a range of finite trace monitoring logics, including future and past time temporal logic, extended regular expressions, real-time and metric temporal logics, interval logics, forms of quantified temporal logics, and so on. A monitor for an EAGLE formula checks if a finite trace of states satisfies the given formula. We present, in details, an algorithm for the synthesis of monitors for EAGLE. The algorithm is implemented as a Java application and involves novel techniques for rule definition, manipulation and execution. Monitoring is achieved on a state-by-state basis avoiding any need to store the input trace of states. Our initial experiments have been successful as EAGLE detected a previously unknown bug while testing a planetary rover controller.

Utilization Probability Map for Migrating Bald Eagles in Northeastern North America: A Tool for Siting Wind Energy Facilities and Other Flight Hazards

PubMed Central

Mojica, Elizabeth K.; Watts, Bryan D.; Turrin, Courtney L.

2016-01-01

Collisions with anthropogenic structures are a significant and well documented source of mortality for avian species worldwide. The bald eagle (Haliaeetus leucocephalus) is known to be vulnerable to collision with wind turbines and federal wind energy guidelines include an eagle risk assessment for new projects. To address the need for risk assessment, in this study, we 1) identified areas of northeastern North America utilized by migrating bald eagles, and 2) compared these with high wind-potential areas to identify potential risk of bald eagle collision with wind turbines. We captured and marked 17 resident and migrant bald eagles in the northern Chesapeake Bay between August 2007 and May 2009. We produced utilization distribution (UD) surfaces for 132 individual migration tracks using a dynamic Brownian bridge movement model and combined these to create a population wide UD surface with a 1 km cell size. We found eagle migration movements were concentrated within two main corridors along the Appalachian Mountains and the Atlantic Coast. Of the 3,123 wind turbines ≥100 m in height in the study area, 38% were located in UD 20, and 31% in UD 40. In the United States portion of the study area, commercially viable wind power classes overlapped with only 2% of the UD category 20 (i.e., the areas of highest use by migrating eagles) and 4% of UD category 40. This is encouraging because it suggests that wind energy development can still occur in the study area at sites that are most viable from a wind power perspective and are unlikely to cause significant mortality of migrating eagles. In siting new turbines, wind energy developers should avoid the high-use migration corridors (UD categories 20 & 40) and focus new wind energy projects on lower-risk areas (UD categories 60–100). PMID:27336482

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

Since January 1994, the Predatory Bird Research Group, University of California, Santa Cruz, has been conducting a field investigation of the ecology of golden eagles (Aquila chrysaetos) in the vicinity of the Altamont Pass Wind Resource Area (WRA). The 190 km{sup 2} facility lies just east of San Francisco Bay in California and contains about 6,500 wind turbines. Grassland and oak savanna habitats surrounding the WRA support a substantial resident population of golden eagles. Each year, the U.S. Fish and Wildlife Service receivers reports from the wind industry of about 30 golden eagle casualties occurring at the WRA, and itmore » is probable that many more carcasses go unnoticed. Over 90 percent of the casualties are attributed to collisions with wind turbines. The main purpose of this study is to estimate the effect of turbine-related mortality on the golden eagle population of the area. Assessing the impact of the WRA kills on the population requires quantification of both survival and reproduction. To estimate survival rates of both territorial and non-territorial golden eagles, we tagged 179 individuals with radio-telemetry transmitters expected to function for about four years and equipped with mortality sensors. Population segments represented in the tagged sample include 79 juveniles, 45 subadults, 17n floaters (non-territorial adults), and 38 breeders. Effective sample sizes in the older segments increase as younger eagles mature or become territorial. Since the beginning of the study, we have conducted weekly roll-call surveys by airplane to locate the tagged eagles in relation to the WRA and to monitor their survival. The surveyed area extends from the Oakland Hills southeast through the Diablo Mountain Range to San Luis Reservoir about 75 km southeast of the WRA. The surveys show that breeding eagles rarely enter the WRA while the non-territorial eagles tend to move about freely throughout the study area and often visit the WRA.« less

Bald eagles and sea otters in the Aleutian Archipelago: indirect effects of trophic cascades.

USGS Publications Warehouse

Anthony, R.G.; Estes, J.A.; Ricca, M.A.; Miles, A.K.; Forsman, E.D.

2008-01-01

Because sea otters (Enhydra lutris) exert a wide array of direct and indirect effects on coastal marine ecosystems throughout their geographic range, we investigated the potential influence of sea otters on the ecology of Bald Eagles (Haliaeetus leucocephalus) in the Aleutian Islands, Alaska, USA. We studied the diets, productivity, and density of breeding Bald Eagles on four islands during 1993–1994 and 2000–2002, when sea otters were abundant and scarce, respectively. Bald Eagles depend on nearshore marine communities for most of their prey in this ecosystem, so we predicted that the recent decline in otter populations would have an indirect negative effect on diets and demography of Bald Eagles. Contrary to our predictions, we found no effects on density of breeding pairs on four islands from 1993–1994 to 2000–2002. In contrast, diets and diet diversity of Bald Eagles changed considerably between the two time periods, likely reflecting a change in prey availability resulting from the increase and subsequent decline in sea otter populations. The frequency of sea otter pups, rock greenling (Hexagammus lagocephalus), and smooth lumpsuckers (Aptocyclus ventricosus) in the eagle's diet declined with corresponding increases in Rock Ptarmigan (Lagopus mutus), Glaucous-winged Gulls (Larus glaucescens), Atka mackerel (Pleurogrammus monopterygius), and various species of seabirds during the period of the recent otter population decline. Breeding success and productivity of Bald Eagles also increased during this time period, which may be due to the higher nutritional quality of avian prey consumed in later years. Our results provide further evidence of the wide-ranging indirect effects of sea otter predation on nearshore marine communities and another apex predator, the Bald Eagle. Although the indirect effects of sea otters are widely known, this example is unique because the food-web pathway transcended five species and several trophic levels in linking one apex predator to another.

West Nile Virus transmission in winter: the 2013 Great Salt Lake Bald Eagle and Eared Grebes Mortality event

USGS Publications Warehouse

Ip, Hon S.; Van Wettere, Arnaud J.; McFarlan, Leslie; Shearn-Bochsler, Valerie I.; Dickson, Sammie L.; Baker, JoDee; Hatch, Gary; Cavender, Kimberly; Long, Renee Romaine; Bodenstein, Barbara L.

2014-01-01

West Nile Virus (WNV) infection has been reported in over 300 species of birds and mammals. Raptors such as eagles, hawks and falcons are remarkably susceptible, but reports of WNV infection in Bald Eagles (Haliaeetus leucocephalus) are rare and reports of WNV infection in grebes (Podicipediformes) even rarer. We report an unusually large wild bird mortality event involving between 15,000-20,000 Eared Grebes (Podiceps nigricollis) and over 40 Bald Eagles around the Great Salt Lake, Utah, in November-December 2013. Mortality in grebes was first reported in early November during a period when the area was unseasonably warm and the grebes were beginning to gather and stage prior to migration. Ten out of ten Eared Grebes collected during this period were WNV RT-PCR and/or isolation positive. This is the first report of WNV infection in Eared Grebes and the associated mortality event is matched in scale only by the combined outbreaks in American White Pelican (Pelecanus erythrorhynchos) colonies in the north central states in 2002-2003. We cannot be sure that all of the grebes were infected by mosquito transmission; some may have become infected through contact with WNV shed orally or cloacally from other infected grebes. Beginning in early December, Bald Eagles in the Great Salt Lake area were observed to display neurological signs such as body tremors, limb paralysis and lethargy. At least 43 Bald Eagles had died by the end of the month. Nine of nine Bald Eagles examined were infected with WNV. To the best of our knowledge, this is the largest single raptor mortality event since WNV became endemic in the USA. Because the majority of the eagles affected were found after onset of below-freezing temperatures, we suggest at least some of the Bald Eagles were infected with WNV via consumption of infected Eared Grebes or horizontal transmission at roost sites.

Geochemical and mineralogical characterization of the Eagle Ford Shale: Results from the USGS Gulf Coast #1 West Woodway core

USGS Publications Warehouse

Birdwell, Justin E.; Boehlke, Adam; Paxton, Stanley T.; Whidden, Katherine J.; Pearson, Ofori N.

2017-01-01

The Eagle Ford shale is a major continuous oil and gas resource play in southcentral Texas and a source for other oil accumulations in the East Texas Basin. As part of the U.S. Geological Survey’s (USGS) petroleum system assessment and research efforts, a coring program to obtain several immature, shallow cores from near the outcrop belt in central Texas has been undertaken. The first of these cores, USGS Gulf Coast #1 West Woodway, was collected near Waco, Texas, in September 2015 and has undergone extensive geochemical and mineralogical characterization using routine methods to ascertain variations in the lithologies and chemofacies present in the Eagle Ford at this locale. Approximately 270 ft of core was examined for this study, focusing on the Eagle Ford Group interval between the overlying Austin Chalk and underlying Buda Limestone (~20 ft of each). Based on previous work to identify the stratigraphy of the Eagle Ford Group in the Waco area and elsewhere (Liro et al., 1994; Robison, 1997; Ratcliffe et al., 2012; Boling and Dworkin, 2015; Fairbanks et al., 2016, and references therein), several lithological units were expected to be present, including the Pepper Shale (or Woodbine), the Lake Waco Formation (or Lower Eagle Ford, including the Bluebonnet, Cloice, and Bouldin or Flaggy Cloice members), and the South Bosque Member (Upper Eagle Ford). The results presented here indicate that there are three major chemofacies present in the cored interval, which are generally consistent with previous descriptions of the Eagle Ford Group in this area. The relatively high-resolution sampling (every two ft above the Buda, 432.8 ft depth, and below the Austin Chalk, 163.5 ft depth) provides great detail in terms of geochemical and mineralogical properties supplementing previous work on immature Eagle Ford Shale near the outcrop belt.

Count trends for migratory Bald Eagles reveal differences between two populations at a spring site along the Lake Ontario shoreline

PubMed Central

2016-01-01

The recovery of Bald Eagles (Haliaeetus leucophalus), after DDT and other organochlorine insecticides were banned in the United States, can be regarded as one of the most iconic success stories resulting from the Endangered Species Act. Interest remains high in the recovery and growth of the Bald Eagle population. Common to evaluating growth and recovery rates are counts at nesting sites and analyses of individuals fledged per season. But this is merely one snapshot that ignores survival rates as eagles grow to maturity. By analyzing indices from migration counts, we get a different snapshot better reflecting the survival of young birds. Different populations of Bald Eagles breed at different sites at different times of the year. Typical migration count analyses do not separate the populations. A separation of two distinct populations can be achieved at spring count sites by taking advantage of the tendency for northern summer breeding birds to migrate north in spring earlier than southern winter breeding birds who disperse north later in spring. In this paper I analyze migratory indices at a spring site along Lake Ontario. The analysis shows that eagles considered to be primarily of the northern summer breeding population showed an estimated growth rate of 5.3 ± 0.85% (SE) per year with 49% of eagles tallied in adult plumage, whereas the migrants considered to be primarily of the southern breeding population had an estimated growth rate of 14.0 ± 1.79% with only 22% in adult plumage. Together these results argue that the populations of southern breeding Bald Eagles are growing at a substantially higher rate than northern breeding eagles. These findings suggest that aggregate population indices for a species at migration counting sites can sometimes obscure important differences among separate populations at any given site and that separating counts by time period can be a useful way to check for differences among sub-populations. PMID:27231647

West Nile Virus Transmission in Winter: The 2013 Great Salt Lake Bald Eagle and Eared Grebes Mortality Event

PubMed Central

Ip, Hon S.; Van Wettere, Arnaud J.; McFarlane, Leslie; Shearn-Bochsler, Valerie; Dickson, Sammie Lee; Baker, JoDee; Hatch, Gary; Cavender, Kimberly; Long, Renee; Bodenstein, Barbara

2014-01-01

West Nile Virus (WNV) infection has been reported in over 300 species of birds and mammals. Raptors such as eagles, hawks and falcons are remarkably susceptible, but reports of WNV infection in Bald Eagles (Haliaeetus leucocephalus) are rare and reports of WNV infection in grebes (Podicipediformes) even rarer. We report an unusually large wild bird mortality event involving between 15,000-20,000 Eared Grebes (Podiceps nigricollis) and over 40 Bald Eagles around the Great Salt Lake, Utah, in November-December 2013. Mortality in grebes was first reported in early November during a period when the area was unseasonably warm and the grebes were beginning to gather and stage prior to migration. Ten out of ten Eared Grebes collected during this period were WNV RT-PCR and/or isolation positive. This is the first report of WNV infection in Eared Grebes and the associated mortality event is matched in scale only by the combined outbreaks in American White Pelican (Pelecanus erythrorhynchos) colonies in the north central states in 2002-2003. We cannot be sure that all of the grebes were infected by mosquito transmission; some may have become infected through contact with WNV shed orally or cloacally from other infected grebes. Beginning in early December, Bald Eagles in the Great Salt Lake area were observed to display neurological signs such as body tremors, limb paralysis and lethargy. At least 43 Bald Eagles had died by the end of the month. Nine of nine Bald Eagles examined were infected with WNV. To the best of our knowledge, this is the largest single raptor mortality event since WNV became endemic in the USA. Because the majority of the eagles affected were found after onset of below-freezing temperatures, we suggest at least some of the Bald Eagles were infected with WNV via consumption of infected Eared Grebes or horizontal transmission at roost sites. PMID:24761310

Utilization Probability Map for Migrating Bald Eagles in Northeastern North America: A Tool for Siting Wind Energy Facilities and Other Flight Hazards.

PubMed

Mojica, Elizabeth K; Watts, Bryan D; Turrin, Courtney L

2016-01-01

Collisions with anthropogenic structures are a significant and well documented source of mortality for avian species worldwide. The bald eagle (Haliaeetus leucocephalus) is known to be vulnerable to collision with wind turbines and federal wind energy guidelines include an eagle risk assessment for new projects. To address the need for risk assessment, in this study, we 1) identified areas of northeastern North America utilized by migrating bald eagles, and 2) compared these with high wind-potential areas to identify potential risk of bald eagle collision with wind turbines. We captured and marked 17 resident and migrant bald eagles in the northern Chesapeake Bay between August 2007 and May 2009. We produced utilization distribution (UD) surfaces for 132 individual migration tracks using a dynamic Brownian bridge movement model and combined these to create a population wide UD surface with a 1 km cell size. We found eagle migration movements were concentrated within two main corridors along the Appalachian Mountains and the Atlantic Coast. Of the 3,123 wind turbines ≥100 m in height in the study area, 38% were located in UD 20, and 31% in UD 40. In the United States portion of the study area, commercially viable wind power classes overlapped with only 2% of the UD category 20 (i.e., the areas of highest use by migrating eagles) and 4% of UD category 40. This is encouraging because it suggests that wind energy development can still occur in the study area at sites that are most viable from a wind power perspective and are unlikely to cause significant mortality of migrating eagles. In siting new turbines, wind energy developers should avoid the high-use migration corridors (UD categories 20 & 40) and focus new wind energy projects on lower-risk areas (UD categories 60-100).

Removal of transmissible spongiform encephalopathy prion from large volumes of cell culture media supplemented with fetal bovine serum by using hollow fiber anion-exchange membrane chromatography.

PubMed

Chou, Ming Li; Bailey, Andy; Avory, Tiffany; Tanimoto, Junji; Burnouf, Thierry

2015-01-01

Cases of variant Creutzfeldt-Jakob disease in people who had consumed contaminated meat products from cattle with bovine spongiform encephalopathy emphasize the need for measures aimed at preventing the transmission of the pathogenic prion protein (PrPSc) from materials derived from cattle. Highly stringent scrutiny is required for fetal bovine serum (FBS), a growth-medium supplement used in the production of parenteral vaccines and therapeutic recombinant proteins and in the ex vivo expansion of stem cells for transplantation. One such approach is the implementation of manufacturing steps dedicated to removing PrPSc from materials containing FBS. We evaluated the use of the QyuSpeed D (QSD) adsorbent hollow-fiber anion-exchange chromatographic column (Asahi Kasei Medical, Tokyo, Japan) for the removal of PrPSc from cell culture media supplemented with FBS. We first established that QSD filtration had no adverse effect on the chemical composition of various types of culture media supplemented with 10% FBS or the growth and viability characteristics of human embryonic kidney (HEK293) cells, baby hamster kidney (BHK-21) cells, African green monkey kidney (Vero) cells, and Chinese hamster ovary (CHO-k1) cells propagated in the various culture-medium filtrates. We used a 0.6-mL QSD column for removing PrPSc from up to 1000 mL of Dulbecco's modified Eagle's medium containing 10% FBS previously spiked with the 263K strain of hamster-adapted scrapie. The Western blot analysis, validated alongside an infectivity assay, revealed that the level of PrPSc in the initial 200mL flow-through was reduced by 2.5 to > 3 log10, compared with that of the starting material. These results indicate that QSD filtration removes PrPSc from cell culture media containing 10% FBS, and demonstrate the ease with which QSD filtration can be implemented in at industrial-scale to improve the safety of vaccines, therapeutic recombinant proteins, and ex vivo expanded stem cells produced using growth media supplemented with FBS.

Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.

PubMed

Liu, Shuchang; Ruban, Ludmila; Wang, Yaohe; Zhou, Yuhong; Nesbeth, Darren N

2017-02-01

Vaccinia virus (VACV) is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits of the VACV genome. Here we take first steps toward establishing a scalable synthetic biology platform for VACV production with CV-1 cells featuring standardised biological tools and serum free cell cultivation. We propose a new BioBrick™ plasmid backbone format for inserting transgenes into VACV. We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS) Dulbecco's Modified Eagle Medium (DMEM) were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h -1 and 0.044 h -1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h -1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. Virus productivity in static culture, measured as plaque forming units (PFU) per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was observed during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies.

Reproduction and distribution of bald eagles in Voyageurs National Park, Minnesota, 1973-1993

USGS Publications Warehouse

Grim, Leland H.; Kallemeyn, Larry W.

1995-01-01

The bald eagle (Haliaeetus leucocephalus) is classified as a threatened species in Minnesota. In 1973, the National Park Service began monitoring the distribution and reproduction of bald eagles in and immediately adjacent to Voyageurs National Park to obtain data that park management could use to protect bald eagles from the effects of use of the park by visitors and from the expansion of park facilities. Thirty-seven breeding areas were identified during 1973-93. Annual productivity ranged from 0.00 to 1.42 fledglings/occupied nest and averaged 0.68 during the 21 breeding seasons. The annual number of breeding pairs tripled, the mean number of fledged eaglets increased 5 times, and reproductive success doubled during the study. However, in more than 15 of the breeding seasons, the mean productivity and the annual reproductive success in Voyageurs National Park were below the 1 fledgling/occupied nest and the 70% reproductive success that are representative of healthy bald eagle populations. We suspect that toxic substances, human disturbance, severe weather, and lack of food in early spring may have kept bald eagles in Voyageurs National Park from achieving a breeding success that was similar to that of conspecifics in the nearby Chippewa National Forest. The cumulative effect of these variables on reproduction and on habitat of bald eagles in Voyageurs National Park is unknown and should be determined.

Known breeding distribution and abundance of golden eagles in Eastern North America

Treesearch

Francois Morneau; Junior A. Tremblay; Charles Todd; Tony E. Chubbs; Charles Maisonneuve; Jerome Lemaitre; Todd Katzner

2015-01-01

Aquila chrysaetos (Golden Eagle) breeds in both eastern and western North America. However, the former population has received much less attention than the latter. The purpose of this paper is to document the known distribution and abundance of eastern Golden Eagles within their breeding range and to identify gaps in knowledge for future studies....

Publications - PIR 2001-3C | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2001-3C Publication Details Title: Surficial-geologic map of the Eagle A-2 Quadrangle publication sales page for more information. Quadrangle(s): Eagle Bibliographic Reference Pinney, D.S., 2001 , Surficial-geologic map of the Eagle A-2 Quadrangle, Fortymile mining district, Alaska: Alaska Division of

Publications - PIR 2001-3A | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2001-3A Publication Details Title: Geologic map of the Eagle A-2 Quadrangle, Fortymile ): Eagle Bibliographic Reference Werdon, M.B., Newberry, R.J., Szumigala, D.J., and Pinney, D.S., 2001 , Geologic map of the Eagle A-2 Quadrangle, Fortymile mining district, Alaska: Alaska Division of Geological

Publications - PIR 2001-3B | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2001-3B Publication Details Title: Bedrock geologic map of the Eagle A-2 Quadrangle more information. Quadrangle(s): Eagle Bibliographic Reference Werdon, M.B., Newberry, R.J., and Szumigala, D.J., 2001, Bedrock geologic map of the Eagle A-2 Quadrangle, Fortymile mining district, Alaska

Publications - PIR 2002-1D | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2002-1D Publication Details Title: Engineering - geologic map of the Eagle A-1 Quadrangle please see our publication sales page for more information. Quadrangle(s): Eagle Bibliographic Reference Stevens, D.S.P., 2012, Engineering - geologic map of the Eagle A-1 Quadrangle, Fortymile mining district

77 FR 43662 - Price for the 2012 American Eagle Silver Proof Coin

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-25

... DEPARTMENT OF THE TREASURY United States Mint Price for the 2012 American Eagle Silver Proof Coin... is lowering the price of the 2012 American Eagle Silver Proof Coin. The product will now be offered for sale at a price of $54.95. FOR FURTHER INFORMATION CONTACT: B. B. Craig, Associate Director for...

77 FR 28375 - Eagle Rock Desoto Pipeline, L.P.; Notice of Petition for Rate Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR12-25-000] Eagle Rock Desoto Pipeline, L.P.; Notice of Petition for Rate Approval Take notice that on May 1, 2012, Eagle Rock Desoto Pipeline, L.P. (Desoto) filed a Rate Election pursuant to 284.123(b)(1) of the Commissions...

50 CFR 22.2 - What activities does this part apply to?

Code of Federal Regulations, 2012 CFR

2012-10-01

...: (i) Any live or dead bald eagles, or their parts, nests, or eggs that were lawfully acquired before June 8, 1940; and (ii) Any live or dead golden eagles, or their parts, nests, or eggs that were... transport into or out of the United States any lawfully acquired dead bald or golden eagles, their parts...

50 CFR 22.2 - What activities does this part apply to?

Code of Federal Regulations, 2010 CFR

2010-10-01

...: (i) Any live or dead bald eagles, or their parts, nests, or eggs that were lawfully acquired before June 8, 1940; and (ii) Any live or dead golden eagles, or their parts, nests, or eggs that were... transport into or out of the United States any lawfully acquired dead bald or golden eagles, their parts...

Nature Photography - Bald Eagle

NASA Image and Video Library

2016-12-12

An American bald eagle soars from its perch in a tree at NASA's Kennedy Space Center in Florida. Several eagles call the center home. The center shares a boundary with the Merritt Island National Wildlife Refuge. The refuge is home to more than 65 amphibian and reptile species, along with 330 native and migratory bird species, 25 mammal and 117 fish species.

50 CFR 22.2 - What activities does this part apply to?

Code of Federal Regulations, 2013 CFR

2013-10-01

...: (i) Any live or dead bald eagles, or their parts, nests, or eggs that were lawfully acquired before June 8, 1940; and (ii) Any live or dead golden eagles, or their parts, nests, or eggs that were... transport into or out of the United States any lawfully acquired dead bald or golden eagles, their parts...

50 CFR 22.2 - What activities does this part apply to?

Code of Federal Regulations, 2011 CFR

2011-10-01

...: (i) Any live or dead bald eagles, or their parts, nests, or eggs that were lawfully acquired before June 8, 1940; and (ii) Any live or dead golden eagles, or their parts, nests, or eggs that were... transport into or out of the United States any lawfully acquired dead bald or golden eagles, their parts...

50 CFR 22.2 - What activities does this part apply to?

Code of Federal Regulations, 2014 CFR

2014-10-01

...: (i) Any live or dead bald eagles, or their parts, nests, or eggs that were lawfully acquired before June 8, 1940; and (ii) Any live or dead golden eagles, or their parts, nests, or eggs that were... transport into or out of the United States any lawfully acquired dead bald or golden eagles, their parts...

Leaders Hit the Battlefield for Education's Future: 2009 Eagle Institute

ERIC Educational Resources Information Center

Verardi, Nicole

2010-01-01

More than 40 esteemed school business officials traveled to Washington, D.C., for the 2009 Eagle Institute which was held on July 14-17. They examined the past and the future to uncover leadership insights. Eagle Institute participants shared a powerful experience of camaraderie, reflection, and optimism for the future. This article describes the…

78 FR 24816 - Pricing for the 2013 American Eagle West Point Two-Coin Silver Set

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-26

... DEPARTMENT OF THE TREASURY United States Mint Pricing for the 2013 American Eagle West Point Two-Coin Silver Set AGENCY: United States Mint, Department of the Treasury. ACTION: Notice. SUMMARY: The United States Mint is announcing the price of the 2013 American Eagle West Point Two-Coin Silver Set. The...

78 FR 57444 - Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-18

... SMALL BUSINESS ADMINISTRATION [License No. 07/07-0117] Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle..., Financings which Constitute Conflicts of Interest, of the Small Business Administration (``SBA'') Rules and...

50 CFR 22.11 - What is the relationship to other permit requirements?

Code of Federal Regulations, 2011 CFR

2011-10-01

... leucocephalus) or any golden eagle (Aquila chrysaetos), or the parts, nests, or eggs of such birds, except as... covers take of bald eagles or golden eagles under 50 CFR part 17 for purposes of providing prospective or... 21 of this subchapter for any activity that also involves migratory birds other than bald and golden...

The genome sequence of a widespread apex Predator, the golden eagle (Aquila chrysaetos)

Treesearch

Jacqueline M. Doyle; Todd E. Katzner; Peter H. Bloom; Yanzhu Ji; Bhagya K. Wijayawardena; J. Andrew DeWoody; Ludovic Orlando

2014-01-01

Biologists routinely use molecular markers to identify conservation units, to quantify genetic connectivity, to estimate population sizes, and to identify targets of selection. Many imperiled eagle populations require such efforts and would benefit from enhanced genomic resources. We sequenced, assembled, and annotated the first eagle genome using DNA from a male...

Growing Leaders in Native American Communities: An Interview with Gerald Eagle Bear

ERIC Educational Resources Information Center

Hill, Sara

2006-01-01

In the summer of 2005, I interviewed Gerald Eagle Bear about his work to promote civic and cultural engagement among Native American youth. Eagle Bear is program manager of the Oyate Networking Project, an affiliate of Christian Children's Fund, in Mission, South Dakota. The organization focuses on early childhood education, youth violence…

27. Historic American Buildings Survey Thomas Paine, Photographer 1969 PHOTOCOPY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. Historic American Buildings Survey Thomas Paine, Photographer 1969 PHOTOCOPY OF ORIGINAL PHOTOGRAPH, TAKEN BETWEEN 1850 AND 1860, SHOWING THE NORTH ELEVATION OF THE CROWN AND EAGLE MILLS From the Crown and Eagle Mills Collection c/o Mr. Kent Robinson, North Uxbridge, Massachusetts - Crown & Eagle Mills, 123 Hartford Avenue East, North Uxbridge, Worcester County, MA

Nature Photography - Bald Eagles

NASA Image and Video Library

2017-01-04

One American bald eagle sits in its nest, while another eagle perches on a branch in tree at NASA's Kennedy Space Center in Florida. The center shares a border with the Merritt Island National Wildlife Refuge. More than 330 native and migratory bird species, 25 mammals, 117 fishes and 65 amphibians and reptiles call Kennedy and the wildlife refuge home.

Research note: isolation of a herpesvirus from a bald eagle nestling

USGS Publications Warehouse

Docherty, D.E.; Romaine, R.I.; Knight, R.L.

1983-01-01

Cloacal swabs collected from wild bald eagle nestlings (Haliaeetus leucocephalus) were tested for viruses. A virus isolated from one of these samples had a lipid coat and contained DNA. Electron microscopy confirmed that it was a herpesvirus. This appears to be the first report of a herpesvirus isolation from a wild bald eagle.

Constructing bald eagle nests with natural materials

Treesearch

T. G. Grubb

1995-01-01

A technique for using natural materials to build artificial nests for bald eagles (Haliaeetus leucocephalus) and other raptors is detailed. Properly constructed nests are as permanently secured to the nest tree or cliff substrate as any eagle-built nest or human-made platform. Construction normally requires about three hours and at least two people. This technique is...

Eagle Feathers, the Highest Honor.

ERIC Educational Resources Information Center

Beaverhead, Pete

Following his own advice that elders of the tribe share their knowledge so that "the way of the Indians would come back to the children of today," Pete Beaverhead (1899-1975) tells of the traditions of respect and honor surrounding the eagle feather in a booklet illustrated with black and white drawings. The eagle is an Indian symbol of…

Bottom-up processes drive reproductive success in an apex predator.

PubMed

Schmidt, Joshua H; McIntyre, Carol L; Roland, Carl A; MacCluskie, Margaret C; Flamme, Melanie J

2018-02-01

One of the central goals of the field of population ecology is to identify the drivers of population dynamics, particularly in the context of predator-prey relationships. Understanding the relative role of top-down versus bottom-up drivers is of particular interest in understanding ecosystem dynamics. Our goal was to explore predator-prey relationships in a boreal ecosystem in interior Alaska through the use of multispecies long-term monitoring data. We used 29 years of field data and a dynamic multistate site occupancy modeling approach to explore the trophic relationships between an apex predator, the golden eagle, and cyclic populations of the two primary prey species available to eagles early in the breeding season, snowshoe hare and willow ptarmigan. We found that golden eagle reproductive success was reliant on prey numbers, but also responded prior to changes in the phase of the snowshoe hare population cycle and failed to respond to variation in hare cycle amplitude. There was no lagged response to ptarmigan populations, and ptarmigan populations recovered quickly from the low phase. Together, these results suggested that eagle reproduction is largely driven by bottom-up processes, with little evidence of top-down control of either ptarmigan or hare populations. Although the relationship between golden eagle reproductive success and prey abundance had been previously established, here we established prey populations are likely driving eagle dynamics through bottom-up processes. The key to this insight was our focus on golden eagle reproductive parameters rather than overall abundance. Although our inference is limited to the golden eagle-hare-ptarmigan relationships we studied, our results suggest caution in interpreting predator-prey abundance patterns among other species as strong evidence for top-down control.

Minimising Mortality in Endangered Raptors Due to Power Lines: The Importance of Spatial Aggregation to Optimize the Application of Mitigation Measures

PubMed Central

Guil, Francisco; Fernández-Olalla, Mariana; Moreno-Opo, Rubén; Mosqueda, Ignacio; Gómez, María Elena; Aranda, Antonio; Arredondo, Ángel; Guzmán, José; Oria, Javier; González, Luis Mariano; Margalida, Antoni

2011-01-01

Electrocution by power lines is one of the main causes of non-natural mortality in birds of prey. In an area in central Spain, we surveyed 6304 pylons from 333 power lines to determine electrocution rates, environmental and design factors that may influence electrocution and the efficacy of mitigation measures used to minimise electrocution cases. A total of 952 electrocuted raptors, representing 14 different species, were observed. Electrocuted raptors were concentrated in certain areas and the environmental factors associated with increased electrocution events were: greater numbers of prey animals; greater vegetation cover; and shorter distance to roads. The structural elements associated with electrocutions were shorter strings of insulators, one or more phases over the crossarm, cross-shaped design and pylon function. Of the 952 carcasses found, 148 were eagles, including golden eagle (Aquila chrysaetos), Spanish imperial eagle (Aquila adalberti) and Bonelli's eagle (Aquila fasciata). Electrocuted eagles were clustered in smaller areas than other electrocuted raptors. The factors associated with increased eagle electrocution events were: pylons function, shorter strings of insulators, higher slopes surrounding the pylon, and more numerous potential prey animals. Pylons with increased string of insulators had lower raptor electrocution rates than unimproved pylons, although this technique was unsuccessful for eagles. Pylons with cable insulation showed higher electrocution rates than unimproved pylons, both for raptors and eagles, despite this is the most widely used and recommended mitigation measure in several countries. To optimize the application of mitigation measures, our results recommend the substitution of pin-type insulators to suspended ones and elongating the strings of insulators. PMID:22140549

Laying the foundations for a human-predator conflict solution: assessing the impact of Bonelli's eagle on rabbits and partridges.

PubMed

Moleón, Marcos; Sánchez-Zapata, José A; Gil-Sánchez, José M; Barea-Azcón, José M; Ballesteros-Duperón, Elena; Virgós, Emilio

2011-01-01

Predation may potentially lead to negative effects on both prey (directly via predators) and predators (indirectly via human persecution). Predation pressure studies are, therefore, of major interest in the fields of theoretical knowledge and conservation of prey or predator species, with wide ramifications and profound implications in human-wildlife conflicts. However, detailed works on this issue in highly valuable--in conservation terms--Mediterranean ecosystems are virtually absent. This paper explores the predator-hunting conflict by examining a paradigmatic, Mediterranean-wide (endangered) predator-two prey (small game) system. We estimated the predation impact ('kill rate' and 'predation rate', i.e., number of prey and proportion of the prey population eaten, respectively) of Bonelli's eagle Aquila fasciata on rabbit Oryctolagus cuniculus and red-legged partridge Alectoris rufa populations in two seasons (the eagle's breeding and non-breeding periods, 100 days each) in SE Spain. The mean estimated kill rate by the seven eagle reproductive units in the study area was c. 304 rabbits and c. 262 partridges in the breeding season, and c. 237 rabbits and c. 121 partridges in the non-breeding period. This resulted in very low predation rates (range: 0.3-2.5%) for both prey and seasons. The potential role of Bonelli's eagles as a limiting factor for rabbits and partridges at the population scale was very poor. The conflict between game profitability and conservation interest of either prey or predators is apparently very localised, and eagles, quarry species and game interests seem compatible in most of the study area. Currently, both the persecution and negative perception of Bonelli's eagle (the 'partridge-eating eagle' in Spanish) have a null theoretical basis in most of this area.

Abundance of Harpy and Crested Eagles from a reservoir-impact area in the Low- and Mid-Xingu River.

PubMed

Sanaiotti, T M; Junqueira, T G; Palhares, V; Aguiar-Silva, F H; Henriques, L M P; Oliveira, G; Guimarães, V Y; Castro, V; Mota, D; Trombin, D F; Villar, D N A; Lara, K M; Fernandes, D; Castilho, L; Yosheno, E; Alencar, R M; Cesca, L; Dantas, S M; Laranjeiras, T O; Mathias, P C; Mendonça, C V

2015-08-01

In the Brazilian Amazon, two monospecific genera, the Harpy Eagle and Crested Eagle have low densities and are classified by IUCN as Near Threatened due to habitat loss, deforestation, habitat degradation and hunting. In this study, we evaluate occurrence of these large raptors using the environmental surveys database from Belo Monte Hydroelectric Power Plant. Integrating the dataset from two methods, we plotted a distribution map along the Xingu River, including records over a 276-km stretch of river. Terrestrial surveys (RAPELD method) were more efficient for detecting large raptors than standardized aquatic surveys, although the latter were complementary in areas without modules. About 53% of the records were obtained during activities of wildlife rescue/flushing, vegetation suppression or in transit. Between 2012 and 2014, four Harpy Eagles were removed from the wild; two shooting victims, one injured by collision with power lines and one hit by a vehicle. Also, seven nests were mapped. The mean distance between Harpy Eagle records was 15 km along the river channel, with a mean of 20 km between nests near the channel, which allowed us to estimate 20 possible pairs using the alluvial forest, riverine forest and forest fragments. Territories of another ten pairs will probably be affected by inundation of the Volta Grande channel, which is far from the main river. The average distance between Crested Eagle records was 16 km along the river channel. The only nest found was 1.3 km away from a Harpy Eagle nest. The remnant forests are under threat of being replaced by cattle pastures, so we recommend that permanently protected riparian vegetation borders (APP) be guaranteed, and that forest fragments within 5 km of the river be conserved to maintain eagle populations.

Assessing risk to birds from industrial wind energy development via paired resource selection models.

PubMed

Miller, Tricia A; Brooks, Robert P; Lanzone, Michael; Brandes, David; Cooper, Jeff; O'Malley, Kieran; Maisonneuve, Charles; Tremblay, Junior; Duerr, Adam; Katzner, Todd

2014-06-01

When wildlife habitat overlaps with industrial development animals may be harmed. Because wildlife and people select resources to maximize biological fitness and economic return, respectively, we estimated risk, the probability of eagles encountering and being affected by turbines, by overlaying models of resource selection for each entity. This conceptual framework can be applied across multiple spatial scales to understand and mitigate impacts of industry on wildlife. We estimated risk to Golden Eagles (Aquila chrysaetos) from wind energy development in 3 topographically distinct regions of the central Appalachian Mountains of Pennsylvania (United States) based on models of resource selection of wind facilities (n = 43) and of northbound migrating eagles (n = 30). Risk to eagles from wind energy was greatest in the Ridge and Valley region; all 24 eagles that passed through that region used the highest risk landscapes at least once during low altitude flight. In contrast, only half of the birds that entered the Allegheny Plateau region used highest risk landscapes and none did in the Allegheny Mountains. Likewise, in the Allegheny Mountains, the majority of wind turbines (56%) were situated in poor eagle habitat; thus, risk to eagles is lower there than in the Ridge and Valley, where only 1% of turbines are in poor eagle habitat. Risk within individual facilities was extremely variable; on average, facilities had 11% (SD 23; range = 0-100%) of turbines in highest risk landscapes and 26% (SD 30; range = 0-85%) of turbines in the lowest risk landscapes. Our results provide a mechanism for relocating high-risk turbines, and they show the feasibility of this novel and highly adaptable framework for managing risk of harm to wildlife from industrial development. © 2014 Society for Conservation Biology.

High efficiency induction of callus and regeneration of sporophytes of Laminaria japonica (Phaeophyta)

NASA Astrophysics Data System (ADS)

Wang, Xi-Hua; Qin, Song; Li, Xin-Ping; Jiang, Peng; Zeng, Cheng-Kui; Qin, Mei

1998-03-01

Four media (PESI solid, MS liquid, MS solid and ASP-C-I solid medium) were used to induce callus from excised tissues of the kelp Laminaria japonica. Only PESI solid medium and MS solid medium produced calli. Modified MS solid medium supplemented with mannitol (3%,W/V), yeast extract (0.1%, W/V), VB2 (0.5 mg/ml), VB12 (0.5 mg/ml), kinetin (0.108 μg/ml) and NAA (1.860μg/ml) showed much better effect on callus induction than non-modified MS solid medium. After 24 days of induction 75.5% of tissues in PESI solid medium showed callus formation. For modified MS solid medium, after three months of induction 67.3% of tissues dedifferentiated into calli. No callus could be found after five months of induction in either MS liquid or ASP-C-I solid medium. When calli were squashed and cultured in N-P enriched autoclaved seawater, MS liquid medium and ASP12-NTA liquid medium (both modified with kelp extract), differentiation of cells and regeneration of sporophytes were only observed in ASP12-NTA medium supplemented with kelp extract. Gametophyte-like filaments formed first, then eggs were released. It was suggested that sporophyte formation could be a process of parthenogenesis. Sterilization techniques in tissue culture of L. japonica were also tested in this study.

Hemograms for and nutritional condition of migrant bald eagles tested for exposure to lead.

PubMed

Miller, M J; Wayland, M E; Bortolotti, G R

2001-07-01

Plasma proteins, hematocrit, differential blood counts were examined and nutritional condition was estimated for bald eagles (Haliaeetus leucocephalus) trapped (n = 66) during antumn migration, 1994-95 at Galloway Bay (Saskatchewan, Canada), for the purposes of estimating prevalence of exposure to lead. Sex and age differences in hematocrit and plasma proteins were not observed; however, female eagles exhibited larger median absolute heterophil counts than males. Hematologic values were similar to those previously reported from eagles in captivity. Departures from expected hematological values from a healthy population of eagles were not observed in birds with elevated levels of blood lead (> or =0.200 microg/ml). Similarly, nutritional condition was not related to blood-lead concentrations. Therefore, it appears that lead exposure in this population was below a threshold required to indicate toxicological alteration in the hematological values and index of nutritional condition that we measured.

Age of the youngest volcanism at Eagle Lake, northeastern California—40Ar/39Ar and paleomagnetic results

USGS Publications Warehouse

Clynne, Michael A.; Calvert, Andrew T.; Champion, Duane E.; Muffler, L.J.P.; Sawlan, Michael G.; Downs, Drew T.

2017-03-22

The age of the youngest volcanism at Eagle Lake, California, was investigated using stratigraphic, paleomagnetic, and 40Ar/39Ar techniques. The three youngest volcanic lava flows at Eagle Lake yielded ages of 130.0±5.1, 127.5±3.2 and 123.6±18.7 ka, and are statistically indistinguishable. Paleomagnetic results demonstrate that two of the lava flows are very closely spaced in time, whereas the third is different by centuries to at most a few millennia. These results indicate that the basalt lava flows at Eagle Lake are not Holocene in age, and were erupted during an episode of volcanism at about 130–125 ka that is unlikely to have spanned more than a few thousand years. Thus, the short-term potential for subsequent volcanism at Eagle Lake is considered low. 

Hornbills can distinguish between primate alarm calls.

PubMed Central

Rainey, Hugo J.; Zuberbühler, Klaus; Slater, Peter J. B.

2004-01-01

Some mammals distinguish between and respond appropriately to the alarm calls of other mammal and bird species. However, the ability of birds to distinguish between mammal alarm calls has not been investigated. Diana monkeys (Cercopithecus diana) produce different alarm calls to two predators: crowned eagles (Stephanoaetus coronatus) and leopards (Panthera pardus). Yellow-casqued hornbills (Ceratogymna elata) are vulnerable to predation by crowned eagles but are not preyed on by leopards and might therefore be expected to respond to the Diana monkey eagle alarm call but not to the leopard alarm call. We compared responses of hornbills to playback of eagle shrieks, leopard growls, Diana monkey eagle alarm calls and Diana monkey leopard alarm calls and found that they distinguished appropriately between the two predator vocalizations as well as between the two Diana monkey alarm calls. We discuss possible mechanisms leading to these responses. PMID:15209110

Process for modifying the metal ion sorption capacity of a medium

DOEpatents

Lundquist, Susan H.

2002-01-01

A process for modifying a medium is disclosed that includes treating a medium having a metal ion sorption capacity with a solution that includes: A) an agent capable of forming a complex with metal ions; and B) ions selected from the group consisting of sodium ions, potassium ions, magnesium ions, and combinations thereof, to create a medium having an increased capacity to sorb metal ions relative to the untreated medium.

76 FR 13446 - Nittany Bald and Eagle Railroad Company-Operation Exemption-SEDA-COG Joint Rail Authority

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-11

... Eagle Railroad Company-Operation Exemption-SEDA- COG Joint Rail Authority Nittany Bald and Eagle... 0.0 and milepost 1.8 in Castanea, Clinton County, Pa. The line is owned or leased by SEDA-COG Joint Rail Authority (SEDA-COG). N&BE states that the line it proposes to operate is an extension of its...

Publications - PIR 2002-1B | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2002-1B Publication Details Title: Bedrock geologic map of the Eagle A-1 Quadrangle for more information. Quadrangle(s): Eagle Bibliographic Reference Szumigala, D.J., Newberry, R.J ., Werdon, M.B., Athey, J.E., Flynn, R.L., and Clautice, K.H., 2002, Bedrock geologic map of the Eagle A-1

77 FR 32716 - Price for the 2012 American Eagle San Francisco Two-Coin Silver Proof Set

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-01

... DEPARTMENT OF THE TREASURY United States Mint Price for the 2012 American Eagle San Francisco Two...: The United States Mint is announcing the price of the 2012 American Eagle San Francisco Two-Coin Silver Proof Set. The coin set will be offered for sale at a price of $149.95. FOR FURTHER INFORMATION...

75 FR 27774 - Eagle Rock Desoto Pipeline, L.P.; Notice of Rate Election

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-18

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-22-000] Eagle Rock Desoto Pipeline, L.P.; Notice of Rate Election May 11, 2010. Take notice that on May 3, 2010, Eagle Rock Desoto Pipeline, L.P., (Desoto) filed a Notice of Rate Election pursuant to section 284.123(b)(1)(ii) of...

Wintering Golden Eagles on the coastal plain of South Carolina

Treesearch

Mark Vukovich; K.L. Turner; T.E. Grazia; T. Mims; J.C. Beasley; John Kilgo

2015-01-01

Golden Eagles (Aquila chrysaetos) are rare winter residents in eastern North America, with most found along the Appalachian Mountains and few reported on the coastal plain of the Carolinas. We used remote cameras baited with wild pig (Sus scrofa) and white-tailed deer (Odocoileus virginianus) carcasses to detect, age, and individually identify Golden Eagles on the U.S...

Conservation significance of alternative nests of golden eagles

Treesearch

Brian A. Millsap; Teryl G. Grubb; Robert K. Murphy; Ted Swem; James W. Watson

2015-01-01

Golden eagles (Aquila chrysaetos) are long-lived raptors that maintain nesting territories that may be occupied for a century or longer. Within occupied nesting territories there is one nest in which eagles lay their eggs in a given year (i.e., the used nest), but there are usually other nests (i.e., alternative nests). Conservation plans often protect used nests, but...

75 FR 20879 - Notice of Intent To Rule on Request To Release Airport Property at the Eagle County Regional...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... To Release Airport Property at the Eagle County Regional Airport, Eagle, CO AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of Request to Release Airport Property. SUMMARY: The FAA proposes to... provisions of section 125 of the Wendell H. Ford Aviation Investment Reform Act for the 21st Century (AIR 21...

75 FR 31811 - Endangered and Threatened Wildlife and Plants; Post-Delisting Monitoring Plan for Bald Eagle...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

... Species Act (ESA) requires that we implement a system, in cooperation with the States, to monitor... cooperation with the States, to effectively monitor for not less than 5 years the status of all species that... stratified sampling based on density of identified bald eagle nest sites. Our Bald Eagle Monitoring Team will...

50 CFR 22.23 - What are the requirements for permits to take depredating eagles and eagles that pose a risk to...

Code of Federal Regulations, 2011 CFR

2011-10-01

... appropriate Regional Director—Attention: Migratory Bird Permit Office. You can find addresses for the... Bird Permit Office within 10 days following completion of the taking operations or the expiration of... be covered by the permit and the injury complained of is substantial, or that bald or golden eagles...

50 CFR 22.23 - What are the requirements for permits to take depredating eagles and eagles that pose a risk to...

Code of Federal Regulations, 2010 CFR

2010-10-01

... appropriate Regional Director—Attention: Migratory Bird Permit Office. You can find addresses for the... Bird Permit Office within 10 days following completion of the taking operations or the expiration of... be covered by the permit and the injury complained of is substantial, or that bald or golden eagles...

Nature Photography - Bald Eagle

NASA Image and Video Library

2016-12-12

An American bald eagle begins to soar from its perch in a tree at NASA's Kennedy Space Center in Florida. Several eagles call the center home. The center shares a boundary with the Merritt Island National Wildlife Refuge. The refuge is home to more than 65 amphibian and reptile species, along with 330 native and migratory bird species, 25 mammal and 117 fish species.

Organochlorine residues and autopsy data from bald eagles 1966-68

USGS Publications Warehouse

Mulhern, B.M.; Reichel, W.L.; Locke, L.N.; Lamont, T.G.; Belisle, A.A.; Cromartie, E.; Bagley, George E.; Prouty, R.M.

1970-01-01

Sixty-nine bald eagles found moribund or dead in 25 States during 1966-68 were analyzed for pesticide residues. Residues of polychlorinated biphenyls and DDE were detected in all samples of eagle carcasses; residues of dieldrin were detected in 68 and residues of DDD in 64; DDT, heptachlor epoxide, and DCBP were detected less frequently. Eight specimens had levels of dieldrin in the brain within the lethal range, and another probably died of DDT poisoning. Autopsy revealed that illegal shooting was the most frequent cause of mortality of these eagles; electrocution, impact injuries, probable lead poisoning, and infectious avian diseases were other causes of mortality.

Eagle RTS: A design for a regional transport aircraft

NASA Technical Reports Server (NTRS)

Bryer, Paul; Buckles, Jon; Lemke, Paul; Peake, Kirk

1992-01-01

This university design project concerns the Eagle RTS (Regional Transport System), a 66 passenger, twin turboprop aircraft with a range of 836 nautical miles. It will operate with a crew of two pilots and two flight attendents. This aircraft will employ the use of aluminum alloys and composite materials to reduce the aircraft weight and increase aerodynamic efficiency. The Eagle RTS will use narrow body aerodynamics with a canard configuration to improve performance. Leading edge technology will be used in the cockpit to improve flight handling and safety. The Eagle RTS propulsion system will consist of two turboprop engines with a total thrust of approximately 6300 pounds, 3150 pounds thrust per engine, for the cruise configuration. The engines will be mounted on the aft section of the aircraft to increase passenger safety in the event of a propeller failure. Aft mounted engines will also increase the overall efficiency of the aircraft by reducing the aircraft's drag. The Eagle RTS is projected to have a takeoff distance of approximately 4700 feet and a landing distance of 6100 feet. These distances will allow the Eagle RTS to land at the relatively short runways of regional airports.

Bald eagle and osprey

USGS Publications Warehouse

Henny, C.J.; Anthony, R.G.; Pendleton, Beth Giron

1989-01-01

Bald eagles nested in all nine western states during recent years (about 19% of known U.S. population in 1982). The known numbers of nesting pairs in the west increased substantially in the last 10 years and totaled 584 in 1986. Much of the increase was due to more intensive survey efforts, but most biologists cite examples of new palrs establishing nesting territories. In contrast, productivity was relatively stable at 0.9 young produced per occupied territory with small annual fluctuations, a level slightly below the requirement for delisting (1.0 young per occupied territory) by the Pacific States Bald Eagle Recovery Plan. About 4,500 to 6,000 (minimum estimate) bald eagles winter throughout the western United States, which is about 50% of the surveyed population in the contiguous 48 states. Osprey range expansion and population increases have been documented in the West since 1981, when the population was estimated at 1,472 palrs (i.e., about 18% of the U.S. population). Monitoring efforts in the 1980s were not as intensive for ospreys as for bald eagles, but productivity was usually at the upper end of 0.95 to 13 young per occupied territory (a rate generally believed adequate for population stability). Although bald eagle and osprey nesting populations and productivity show cause for optimism, organochlorine contaminants remain a problem in a few individual birds and in some localized areas (e.g., lower Columbia River). DDE residues high enough to reduce productivity have been documented in eggs of both species during the 1980s. In addition, the bald eagle, which also forages on sick or dead prey, has been exposed to lead shot and the organophosphorus insecticide famphur. These contaminants have killed numbers of them in the West in recent years. Nesting ospreys appear more tolerant than nesting bald eagles of man and his disturbance; thus, more restrictions are required at bald eagle nest sites. Furthermore, bald eagles winter within the United States and their specific wintering requirements are generally known and considered in the species' management, whereas western ospreys winter in Mexico and Central America where knowledge of their requirements is nearly nonexistent. Reintroduction worked successfully for both species elsewhere but is not a high ,priority for western localities because few populations were extirpated and most populations have increased.

Estimation of occupancy, breeding success, and predicted abundance of golden eagles (Aquila chrysaetos) in the Diablo Range, California, 2014

USGS Publications Warehouse

Wiens, J. David; Kolar, Patrick S.; Fuller, Mark R.; Hunt, W. Grainger; Hunt, Teresa

2015-01-01

We used a multistate occupancy sampling design to estimate occupancy, breeding success, and abundance of territorial pairs of golden eagles (Aquila chrysaetos) in the Diablo Range, California, in 2014. This method uses the spatial pattern of detections and non-detections over repeated visits to survey sites to estimate probabilities of occupancy and successful reproduction while accounting for imperfect detection of golden eagles and their young during surveys. The estimated probability of detecting territorial pairs of golden eagles and their young was less than 1 and varied with time of the breeding season, as did the probability of correctly classifying a pair’s breeding status. Imperfect detection and breeding classification led to a sizeable difference between the uncorrected, naïve estimate of the proportion of occupied sites where successful reproduction was observed (0.20) and the model-based estimate (0.30). The analysis further indicated a relatively high overall probability of landscape occupancy by pairs of golden eagles (0.67, standard error = 0.06), but that areas with the greatest occupancy and reproductive potential were patchily distributed. We documented a total of 138 territorial pairs of golden eagles during surveys completed in the 2014 breeding season, which represented about one-half of the 280 pairs we estimated to occur in the broader 5,169-square kilometer region sampled. The study results emphasize the importance of accounting for imperfect detection and spatial heterogeneity in studies of site occupancy, breeding success, and abundance of golden eagles.

Assessment of frequency and duration of point counts when surveying for golden eagle presence

USGS Publications Warehouse

Skipper, Ben R.; Boal, Clint W.; Tsai, Jo-Szu; Fuller, Mark R.

2017-01-01

We assessed the utility of the recommended golden eagle (Aquila chrysaetos) survey methodology in the U.S. Fish and Wildlife Service 2013 Eagle Conservation Plan Guidance. We conducted 800-m radius, 1-hr point-count surveys broken into 20-min segments, during 2 sampling periods in 3 areas within the Intermountain West of the United States over 2 consecutive breeding seasons during 2012 and 2013. Our goal was to measure the influence of different survey time intervals and sampling periods on detectability and use estimates of golden eagles among different locations. Our results suggest that a less intensive effort (i.e., survey duration shorter than 1 hr and point-count survey radii smaller than 800 m) would likely be inadequate for rigorous documentation of golden eagle occurrence pre- or postconstruction of wind energy facilities. Results from a simulation analysis of detection probabilities and survey effort suggest that greater temporal and spatial effort could make point-count surveys more applicable for evaluating golden eagle occurrence in survey areas; however, increased effort would increase financial costs associated with additional person-hours and logistics (e.g., fuel, lodging). Future surveys can benefit from a pilot study and careful consideration of prior information about counts or densities of golden eagles in the survey area before developing a survey design. If information is lacking, survey planning may be best served by assuming low detection rates and increasing the temporal and spatial effort.

A pair of bald eagles on a utility pole at KSC.

NASA Technical Reports Server (NTRS)

2000-01-01

A pair of nesting bald eagles share a utility pole on Kennedy Parkway North. Nearby is their 11-foot-deep nest, in a pine tree, which has been home to one or more pairs of eagles for two dozen years. It is one of a dozen eagle nests in the Merritt Island National Wildlife Refuge, which shares a boundary with Kennedy Space Center. The Southern Bald Eagle ranges throughout Florida and along the coasts of California, Texas, Louisiana, and the south Atlantic states. Bald Eagles are listed as endangered in the U.S., except in five states where they are listed as threatened. The number of nesting pairs of the southern race once numbered several thousand; recent estimates are only 350-375. Most of the southern race nests in Florida Eagles arrive at KSC during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. The Refuge encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle, and Peregrine Falcon spermatozoa

USGS Publications Warehouse

Blanco, J.M.; Gee, G.; Wildt, D.E.; Donoghue, A.M.

2000-01-01

Potential factors influencing spermatozoa survival to cryopreservation and thawing were analyzed across a range of the following avian species: domestic chicken (Gallus domesticus), domestic turkey (Meleagris gallopavo), golden eagle (Aquila chrysaetos), Bonelli's eagle (Hieraaetus fasciatus), imperial eagle (Aquila adalberti), and peregrine falcon (Falco peregrinus). Studies focused on spermatozoa tolerance to the following: 1) osmotic stress, 2) different extracellular concentrations of the cryoprotectant dimethylacetamide (DMA), 3) equilibration times of 1 versus 4 h, 4) equilibration temperature of 4 versus 21 degrees C, and 5) rapid versus slow cooling before cryopreservation and standard thawing. Sperm viability was assessed with the live/dead stain (SYBR14/ propidium iodine). Sperm viability at osmolalities >/=800 mOsm was higher (P: /=2.06 M), experienced decreased (P: < 0.05) spermatozoa survival in all species, except the golden eagle and peregrine falcon. Number of surviving spermatozoa diminished progressively with increasing DMA concentrations in all species. Increased equilibration temperature (from 4 to 21 degrees C) markedly reduced (P: < 0.05) spermatozoa survival in all species except the Bonelli's eagle and turkey. Rapid cooling was detrimental (P: < 0.05) to spermatozoa from all species except the imperial eagle and the chicken. These results demonstrate that avian spermatozoa differ remarkably in response to osmotic changes, DMA concentrations, equilibration time, temperature, and survival after fast or slow freezing. These differences emphasize the need for species-specific studies in the development and enhancement of assisted breeding for poultry and endangered species.

Golden eagle population trends in the western United States: 1968-2010

USGS Publications Warehouse

Millsap, Brian A.; Zimmerman, Guthrie S.; Sauer, John R.; Nielson, Ryan M.; Otto, Mark; Bjerre, Emily; Murphy, Robert K.

2013-01-01

In 2009, the United States Fish and Wildlife Service promulgated permit regulations for the unintentional lethal take (anthropogenic mortality) and disturbance of golden eagles (Aquila chrysaetos). Accurate population trend and size information for golden eagles are needed so agency biologists can make informed decisions when eagle take permits are requested. To address this need with available data, we used a log-linear hierarchical model to average data from a late-summer aerial-line-transect distance-sampling survey (WGES) of golden eagles in the United States portions of Bird Conservation Region (BCR) 9 (Great Basin), BCR 10 (Northern Rockies), BCR 16 (Southern Rockies/Colorado Plateau), and BCR 17 (Badlands and Prairies) from 2006 to 2010 with late-spring, early summer Breeding Bird Survey (BBS) data for the same BCRs and years to estimate summer golden eagle population size and trends in these BCRs. We used the ratio of the density estimates from the WGES to the BBS index to calculate a BCR-specific adjustment factor that scaled the BBS index (i.e., birds per route) to a density estimate. Our results indicated golden eagle populations were generally stable from 2006 to 2010 in the 4 BCRs, with an estimated average rate of population change of −0.41% (95% credible interval [CI]: −4.17% to 3.40%) per year. For the 4 BCRs and years, we estimated annual golden eagle population size to range from 28,220 (95% CI: 23,250–35,110) in 2007 to 26,490 (95% CI: 21,760–32,680) in 2008. We found a general correspondence in trends between WGES and BBS data for these 4 BCRs, which suggested BBS data were providing useful trend information. We used the overall adjustment factor calculated from the 4 BCRs and years to scale BBS golden eagle counts from 1968 to 2005 for the 4 BCRs and for 1968 to 2010 for the 8 other BCRs (without WGES data) to estimate golden eagle population size and trends across the western United States for the period 1968 to 2010. In general, we noted slightly declining trends in southern BCRs and slightly increasing trends in northern BCRs. However, we estimated the average rate of golden eagle population change across all 12 BCRs for the period 1968–2010 as +0.40% per year (95% CI = −0.27% to 1.00%), suggesting a stable population. We also estimated the average rate of population change for the period 1990–2010 was +0.5% per year (95% CI = −0.33% to 1.3%). Our annual estimates of population size for the most recent decade range from 31,370 (95% CI: 25,450–39,310) in 2004 to 33,460 (95% CI: 27,380–41,710) in 2007. Our results clarify that golden eagles are not declining widely in the western United States. © 2013 The Wildlife Society.

NORAD, USNORTHCOM and the Mexican Air Force to participate in AMALGAM EAGLE

Science.gov Websites

. Northern Command (USNORTHCOM), will participate, with the Mexican Air Force, in AMALGAM EAGLE 15, a the Mexican Air Force to participate in AMALGAM EAGLE 15 N-NC Public Affairs PRINT | E-MAIL PETERSON tactical exercise, to be conducted Jun.30 - Jul.2, 2015, in which Mexico and the United States will respond

Using nestling plasma to assess long-term spatial and temporal concentrations of organochlorine compounds in bald eagles within Voyageurs National Park, Minnesota, USA

Treesearch

H. Tyler Pittman; William W. Bowerman; Leland H. Grim; Teryl G. Grubb; William C. Bridges; Michael R. Wierda

2015-01-01

The bald eagle (Haliaeetus leucocephalus) population at Voyageurs National Park (VNP) provides an opportunity to assess long-term temporal and spatial trends of persistent environmental contaminants. Nestling bald eagle plasma samples collected from 1997 to 2010 were analyzed for polychlorinated biphenyls (PCBs) and organochlorine pesticides. Trends of total PCBs,...

Predator functional response and prey survival: Direct and indirect interactions affecting a marked prey population

USGS Publications Warehouse

Miller, David A.; Grand, J.B.; Fondell, T.F.; Anthony, M.

2006-01-01

1. Predation plays an integral role in many community interactions, with the number of predators and the rate at which they consume prey (i.e. their functional response) determining interaction strengths. Owing to the difficulty of directly observing predation events, attempts to determine the functional response of predators in natural systems are limited. Determining the forms that predator functional responses take in complex systems is important in advancing understanding of community interactions. 2. Prey survival has a direct relationship to the functional response of their predators. We employed this relationship to estimate the functional response for bald eagle Haliaeetus leucocepalus predation of Canada goose Branta canadensis nests. We compared models that incorporated eagle abundance, nest abundance and alternative prey presence to determine the form of the functional response that best predicted intra-annual variation in survival of goose nests. 3. Eagle abundance, nest abundance and the availability of alternative prey were all related to predation rates of goose nests by eagles. There was a sigmoidal relationship between predation rate and prey abundance and prey switching occurred when alternative prey was present. In addition, predation by individual eagles increased as eagle abundance increased. 4. A complex set of interactions among the three species examined in this study determined survival rates of goose nests. Results show that eagle predation had both prey- and predator-dependent components with no support for ratio dependence. In addition, indirect interactions resulting from the availability of alternative prey had an important role in mediating the rate at which eagles depredated nests. As a result, much of the within-season variation in nest survival was due to changing availability of alternative prey consumed by eagles. 5. Empirical relationships drawn from ecological theory can be directly integrated into the estimation process to determine the mechanisms responsible for variation in observed survival rates. The relationship between predator functional response and prey survival offers a flexible and robust method to advance our understanding of predator-prey interactions in many complex natural systems where prey populations are marked and regularly visited. ?? 2006 British Ecological Society.

A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples.

PubMed

Lima-Nishimura, N; Quoirin, M; Naddaf, Y G; Wilhelm, H M; Ribas, L L F; Sierakowski, M-R

2003-01-01

Xyloglucan was extracted from seeds of Hymenaea courbaril and mixed with agar to prepare a solid culture medium used for micropropagation of the Marubakaido apple rootstock (Malus prunifolia Borkh) and cv. Jonagored (Malus domestica). The performance on gels created from a blend of 0.4%agar and 0.2% xyloglucan (w/v) was compared with that on media gelled with a standard concentration 0.6% (w/v) of agar. The growth of shoots and the multiplication rate were higher on the modified culture medium than on the agar-gelled medium. The occurrence of hyperhydric shoots was lower on the modified medium. In the absence of auxin, shoot rooting reached 70% (Marubakaido) and 66% (Jonagored) on the agar-xyloglucan medium and 6.7% and 10.4%, respectively, on the agar medium. When 0.25 microM indole-3-butyric acid (IBA) was added to both media, the modified medium gave better results in terms of rooting percentage and quality of roots than the agar-gelled medium.

Reduction of Genetic Diversity of the Harpy Eagle in Brazilian Tropical Forests.

PubMed

Banhos, Aureo; Hrbek, Tomas; Sanaiotti, Tânia M; Farias, Izeni Pires

2016-01-01

Habitat loss and fragmentation intensify the effects of genetic drift and endogamy, reducing genetic variability of populations with serious consequences for wildlife conservation. The Harpy Eagle (Harpia harpyja) is a forest dwelling species that is considered near threatened and suffers from habitat loss in the forests of the Neotropical region. In this study, 72 historical and current samples were assessed using eight autosomal microsatellite markers to investigate the distribution of genetic diversity of the Harpy Eagle of the Amazonian and Atlantic forests in Brazil. The results showed that the genetic diversity of Harpy Eagle decreased in the regions where deforestation is intense in the southern Amazon and Atlantic Forest.

Size dimorphism, molt status, and body mass variation of prairiefalcons nesting in the Snake River Birds of Prey National Conservation Area

USGS Publications Warehouse

Steenhof, Karen; McKinley, J.O.

2006-01-01

Thirty-nine bald eagles found sick or dead in 13 States during 1969 and 1970 were analyzed for pesticide residues. Residues of DDE, dieldrin, polychlorinated biphenyls (PCB's), and mercury were detected in all bald eagle carcasses; DDD residues were detected in 38; DDT, heptachlor epoxide, and dichlorobenzophenone (DCBP) were detected less frequently. Six eagles contained possible lethal levels of dieldrin in the brain, and one contained a lethal concentration of DDE (385 ppm) in the brain together with 235 ppm of PCB's. Autopsy revealed that 18 bald eagles were illegally shot; other causes of death were impact injuries, electrocution, emaciation, and infectious diseases.

Assessment of undiscovered oil and gas resources in conventional and continuous petroleum systems in the Upper Cretaceous Eagle Ford Group, U.S. Gulf Coast region, 2011

USGS Publications Warehouse

Dubiel, Russell F.; Pitman, Janet K.; Pearson, Ofori N.; Pearson, Krystal; Kinney, Scott A.; Lewan, Michael D.; Burke, Lauri; Biewick, Laura; Charpentier, Ronald R.; Cook, Troy A.; Klett, Timothy R.; Pollastro, Richard M.; Schenk, Christopher J.

2012-01-01

Using a geology-based assessment methodology, the U.S. Geological Survey assessed means of (1) 141 million barrels of oil (MMBO), 502 billion cubic feet of natural gas (BCFG), and 16 million barrels of natural gas liquids (MMBNGL) in the conventional Eagle Ford Updip Sandstone Oil and Gas Assessment Unit (AU); (2) 853 MMBO, 1,707 BCFG, and 34 MMBNGL in the continuous Eagle Ford Shale Oil AU; and (3) 50,219 BCFG and 2,009 MMBNGL in the continuous Eagle Ford Shale Gas AU in onshore lands and State waters of the Gulf Coast.

Reduction of Genetic Diversity of the Harpy Eagle in Brazilian Tropical Forests

PubMed Central

2016-01-01

Habitat loss and fragmentation intensify the effects of genetic drift and endogamy, reducing genetic variability of populations with serious consequences for wildlife conservation. The Harpy Eagle (Harpia harpyja) is a forest dwelling species that is considered near threatened and suffers from habitat loss in the forests of the Neotropical region. In this study, 72 historical and current samples were assessed using eight autosomal microsatellite markers to investigate the distribution of genetic diversity of the Harpy Eagle of the Amazonian and Atlantic forests in Brazil. The results showed that the genetic diversity of Harpy Eagle decreased in the regions where deforestation is intense in the southern Amazon and Atlantic Forest. PMID:26871719

Scaled Eagle Nebula Experiments on NIF

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pound, Marc W.

We performed scaled laboratory experiments at the National Ignition Facility laser to assess models for the creation of pillar structures in star-forming clouds of molecular hydrogen, in particular the famous Pillars of the Eagle Nebula. Because pillars typically point towards nearby bright ultraviolet stars, sustained directional illumination appears to be critical to pillar formation. The experiments mock up illumination from a cluster of ultraviolet-emitting stars, using a novel long duration (30--60 ns), directional, laser-driven x-ray source consisting of multiple radiation cavities illuminated in series. Our pillar models are assessed using the morphology of the Eagle Pillars observed with the Hubblemore » Space Telescope, and measurements of column density and velocity in Eagle Pillar II obtained at the BIMA and CARMA millimeter wave facilities. In the first experiments we assess a shielding model for pillar formation. The experimental data suggest that a shielding pillar can match the observed morphology of Eagle Pillar II, and the observed Pillar II column density and velocity, if augmented by late time cometary growth.« less

Golden Eagle fatalities and the continental-scale consequences of local wind-energy generation

USGS Publications Warehouse

Katzner, Todd E.; Nelson, David M.; Braham, Melissa A.; Doyle, Jacqueline M.; Fernandez, Nadia B.; Duerr, Adam E.; Bloom, Peter H.; Fitzpatrick, Matthew C.; Miller, Tricia A.; Culver, Renee C. E.; Braswell, Loan; DeWoody, J. Andrew

2017-01-01

Renewable energy production is expanding rapidly despite mostly unknown environmental effects on wildlife and habitats. We used genetic and stable isotope data collected from Golden Eagles (Aquila chrysaetos) killed at the Altamont Pass Wind Resource Area (APWRA) in California in demographic models to test hypotheses about the geographic extent and demographic consequences of fatalities caused by renewable energy facilities. Geospatial analyses of δ2H values obtained from feathers showed that ≥25% of these APWRA-killed eagles were recent immigrants to the population, most from long distances away (>100 km). Data from nuclear genes indicated this subset of immigrant eagles was genetically similar to birds identified as locals from the δ2H data. Demographic models implied that in the face of this mortality, the apparent stability of the local Golden Eagle population was maintained by continental-scale immigration. These analyses demonstrate that ecosystem management decisions concerning the effects of local-scale renewable energy can have continental-scale consequences.

Golden Eagle fatalities and the continental-scale consequences of local wind-energy generation.

PubMed

Katzner, Todd E; Nelson, David M; Braham, Melissa A; Doyle, Jacqueline M; Fernandez, Nadia B; Duerr, Adam E; Bloom, Peter H; Fitzpatrick, Matthew C; Miller, Tricia A; Culver, Renee C E; Braswell, Loan; DeWoody, J Andrew

2017-04-01

Renewable energy production is expanding rapidly despite mostly unknown environmental effects on wildlife and habitats. We used genetic and stable isotope data collected from Golden Eagles (Aquila chrysaetos) killed at the Altamont Pass Wind Resource Area (APWRA) in California in demographic models to test hypotheses about the geographic extent and demographic consequences of fatalities caused by renewable energy facilities. Geospatial analyses of δ 2 H values obtained from feathers showed that ≥25% of these APWRA-killed eagles were recent immigrants to the population, most from long distances away (>100 km). Data from nuclear genes indicated this subset of immigrant eagles was genetically similar to birds identified as locals from the δ 2 H data. Demographic models implied that in the face of this mortality, the apparent stability of the local Golden Eagle population was maintained by continental-scale immigration. These analyses demonstrate that ecosystem management decisions concerning the effects of local-scale renewable energy can have continental-scale consequences. © 2016 Society for Conservation Biology.

Golden Eagle predation on experimental Sandhill and Whooping Cranes

USGS Publications Warehouse

Ellis, D.H.; Clegg, K.R.; Lewis, J.C.; Spaulding, E.

1999-01-01

There are very few published records of Golden Eagles preying upon cranes, especially in North America. During our experiments to lead cranes on migration behind motorized craft in the western United States, we experienced 15 attacks (four fatal) and believe many more attacks would have occurred (and more would have been fatal) without human intervention. We recognize eagle predation as an important risk to cranes especially during migration.

Wildlife Photography - Eagles

NASA Image and Video Library

2018-03-13

An adult American bald eagle perches in a nest in a tree along State Road 3 at NASA's Kennedy Space Center in Florida. Eagles have built nests in trees at the center for many years. The center shares a border with the 140,000-acre Merritt Island National Wildlife Refuge. More than 330 native and migratory bird species, 25 mammals, 117 fishes and 65 amphibians and reptiles call Kennedy and the wildlife refuge home.

Wildlife Photography - Eagles

NASA Image and Video Library

2018-03-13

An adult American bald eagle perches on a branch in a tree along State Road 3 at NASA's Kennedy Space Center in Florida. Eagles have built nests in trees at the center for many years. The center shares a border with the 140,000-acre Merritt Island National Wildlife Refuge. More than 330 native and migratory bird species, 25 mammals, 117 fishes and 65 amphibians and reptiles call Kennedy and the wildlife refuge home.

Experimental lead poisoning in bald eagles

USGS Publications Warehouse

Pattee, H.; Wiemeyer, S.; Hoffman, P.; Carpenter, J.; Sileo, L.

1979-01-01

Captive, crippled bald eagles unsuitable for release were fed lead shot to determine diagnostic criteria for lead poisoning. The eagles were fluoroscoped and bled periodically to determine shot retention and blood delta--aminolevulinic acid dehydratase activity. Microscopic examination revealed renal tubular degeneration, arterial fibrinoid necrosis and myocardial necrosis. Acid-fast intra-nuclear inclusion bodies were not found in proximal convoluted tubule cells. Analyses of blood and toxicological data are not yet complete.

Experimental lead-shot poisoning in bald eagles

USGS Publications Warehouse

Pattee, O.H.; Wiemeyer, Stanley N.; Mulhern, B.M.; Sileo, L.; Carpenter, J.W.

1981-01-01

Captive, crippled bald eagles unsuitable for release were fed lead shot to determine diagnostic criteria for lead poisoning. The eagles were fluoroscoped and bled periodically to determine shot retention and blood delta--aminolevulinic acid dehydratase activity. Microscopic examination revealed renal tubular degeneration, arterial fibrinoid necrosis and myocardial necrosis. Acid-fast intra-nuclear inclusion bodies were not found in proximal convoluted tubule cells. Analyses of blood and toxicological data are not yet complete.

Quest for safer skies: Modeling golden eagles and wind energy to reduce turbine risk

Treesearch

Todd Katzner; Tricia Miller; Scott Stoleson

2014-01-01

In a patch of sky above Pennsylvania, a golden eagle moves languidly, never flapping but passing quickly as it cruises southward on a cushion of air. It is migrating to its wintering grounds after a season of breeding in Quebec. As part of a team studying eagles on a daily basis—a project supported by the U.S. Forest Service (USFS), West Virginia University,...

A Southern Bald Eagle perches on a pole at KSC.

NASA Technical Reports Server (NTRS)

2000-01-01

A Southern Bald Eagle perched on top of a utility pole searches the area. About a dozen bald eagles live in the Merritt Island National Wildlife Refuge, which shares a boundary with Kennedy Space Center. The Southern Bald Eagle ranges throughout Florida and along the coasts of California, Texas, Louisiana, and the south Atlantic states. Bald Eagles are listed as endangered in the U.S., except in five states where they are listed as threatened. The number of nesting pairs of the southern race once numbered several thousand; recent estimates are only 350-375. Most of the southern race nest in Florida. Eagles arrive at KSC during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. The Refuge encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

A Southern Bald Eagle perches on a pole at KSC.

NASA Technical Reports Server (NTRS)

2000-01-01

A Southern Bald Eagle perches on top of a utility pole at Kennedy Space Center. About a dozen bald eagles live in the Merritt Island National Wildlife Refuge, which shares a boundary with Kennedy Space Center. The Southern Bald Eagle ranges throughout Florida and along the coasts of California, Texas, Louisiana, and the south Atlantic states. Bald Eagles are listed as endangered in the U.S., except in five states where they are listed as threatened. The number of nesting pairs of the southern race once numbered several thousand; recent estimates are only 350-375. Most of the southern race nest in Florida. Eagles arrive at KSC during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. The Refuge encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

KSC-07pd0926

NASA Image and Video Library

2007-04-20

KENNEDY SPACE CENTER, FLA. -- This adult bald eagle rests on the ground near a pond close to S.R. 3 in NASA's Kennedy Space Center. Bald eagles live near large bodies of open water such as lakes, marshes, seacoasts and rivers, where there are plenty of fish to eat and tall trees for nesting and roosting. Bald eagles feed primarily on fish, but also eat small animals (ducks, coots, muskrats, turtles, rabbits, snakes, etc.) and occasional carrion (dead animals). They are sometimes seen among a gathering of vultures at the site of a fresh meal. Bald eagles have a presence in every U. S. state except Hawaii. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Its natural domain is from Alaska to Baja, California, and from Maine to Florida. There are a dozen eagle nests both in KSC and in the Merritt Island National Wildlife Refuge, which surrounds KSC. The refuge includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered. Photo credit: NASA/George Shelton

KSC-07pd0925

NASA Image and Video Library

2007-04-20

KENNEDY SPACE CENTER, FLA. -- This adult bald eagle rests on the ground near a pond close to S.R. 3 in NASA's Kennedy Space Center. Bald eagles live near large bodies of open water such as lakes, marshes, seacoasts and rivers, where there are plenty of fish to eat and tall trees for nesting and roosting. Bald eagles feed primarily on fish, but also eat small animals (ducks, coots, muskrats, turtles, rabbits, snakes, etc.) and occasional carrion (dead animals). They are sometimes seen among a gathering of vultures at the site of a fresh meal. Bald eagles have a presence in every U. S. state except Hawaii. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Its natural domain is from Alaska to Baja, California, and from Maine to Florida. There are a dozen eagle nests both in KSC and in the Merritt Island National Wildlife Refuge, which surrounds KSC. The refuge includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered. Photo credit: NASA/George Shelton

Eagle RTS: A design of a regional transport

NASA Technical Reports Server (NTRS)

Bryer, Paul; Buckles, Jon; Lemke, Paul; Peake, Kirk

1992-01-01

The Eagle RTS (Regional Transport System) is a 66-passenger aircraft designed to satisfy the need for accessible and economical regional travel. The first design objective for the Eagle RTS is safety. Safety results primarily from avoidance of the hub airport air traffic, implementation of anti-stall characteristics by tailoring the canard, and proper positioning of the engines for blade shedding. To provide the most economical aircraft, the Eagle RTS will use existing technology to lower production and maintenance costs by decreasing the amount of new training required. In selecting the propulsion system, the effects on the environment were a main consideration. Two advantages of turbo-prop engines are the high fuel efficiency and low noise levels produced by this type of engine. This ensures the aircraft's usage during times of rising fuel costs and growing aircraft noise restrictions. The design of the Eagle RTS is for spoke-to-spoke transportation. It must be capable of landing on shorter runways and have speeds comparable to that of the larger aircraft to make its service beneficial to the airlines. With the use of turbo-prop engines and high lift devices, the Eagle RTS is highly adaptable to regional airports. The design topics discussed include: aerodynamics, stability, structures and materials, propulsion, and cost.

Solar Eagle 2

NASA Technical Reports Server (NTRS)

Roberto, Richard D.

1995-01-01

During a 22-month period from February 1991 to December 1993, a dedicated group of students, faculty, and staff at California State University, Los Angeles completed a project to design, build, and race their second world class solar-powered electric vehicle, the Solar Eagle 2. This is the final report of that project. As a continuation of the momentum created by the success of the GM-sponsored Sunrayce USA in 1990, the U.S. Department of Energy (DOE) picked up the banner from General Motors as sponsors of Sunrayce 93. In February 1991, the DOE sent a request for proposals to all universities in North America inviting them to submit a proposal outlining how they would design, build, and test a solar-powered electric vehicle for a seven-day race from Arlington, Texas to Minneapolis, Minnesota, to be held in June 1993. Some 70 universities responded. At the end of a proposal evaluation process, 36 universities including CSLA were chosen to compete. This report documents the Solar Eagle 2 project--the approaches take, what was learned, and how our experience from the first Solar Eagle was incorporated into Solar Eagle 2. The intent is to provide a document that would assist those who may wish to take up the challenge to build Solar Eagle 3.

Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine.

PubMed

Vouzara, T; Koulaouzidou, E; Ziouti, F; Economides, N

2016-08-01

To evaluate the capacity of commonly used root canal irrigants to induce cytotoxic effects, when applied singly or in combination. The hypothesis tested was that the irrigants were less cytotoxic when applied in combination than independently. MRC5 cells were grown as monolayer cultures at 37 °C in an atmosphere containing 5% CO2 in air and 100% relative humidity. Cells were exposed to sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), chlorhexidine (CHX) and their combinations (NaOCl/EDTA, NaOCl/CHX, EDTA/CHX) in serial dilutions. Growth medium was Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum and antibiotics and was used as control. The effect on cell survival was estimated after 6 and 24 h of exposure by means of the sulforhodamine B assay, in reference to controls. Dose-response curves were plotted, and 50% inhibitory doses (IC50 ) were subjected to statistical analysis (anova and post hoc comparison test; P < 0.05). Analysis of cell survival and interaction of the irrigants was performed using CalcuSyn dose effect analyzer software to calculate a combination index (CI). The tested irrigants were cytotoxic in dose- and time-dependent manner. CHX was the most cytotoxic irrigant tested, followed by NaOCl, whilst EDTA was the least cytotoxic irrigant tested. The difference between CHX and NaOCl was significant (P < 0.05) as well as between NaOCl and EDTA (P < 0.05). Based on CalcuSyn modelling, a mainly antagonistic effect was recorded with NaOCl/CHX and NaOCl/EDTA combinations. The combination EDTA/CHX had an additive to antagonistic effect. CHX was significantly more cytotoxic than NaOCl and EDTA. NaOCl was significantly more cytotoxic than EDTA. The combined action of irrigants did not produce a significant increase in their cytotoxicity. © 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Pooled human platelet lysate versus fetal bovine serum-investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use.

PubMed

Trojahn Kølle, Stig-Frederik; Oliveri, Roberto S; Glovinski, Peter V; Kirchhoff, Maria; Mathiasen, Anders Bruun; Elberg, Jens Jørgen; Andersen, Peter Stemann; Drzewiecki, Krzysztof Tadeusz; Fischer-Nielsen, Anne

2013-09-01

Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEM(pHPL) versus DMEM(FBS)). ASCs from four healthy donors were cultured in either DMEM(pHPL) or DMEM(FBS), and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEM(pHPL) or DMEM(FBS) and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells. The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor. We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity. Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

Metabolic activity of odontoblast-like cells irradiated with blue LED (455 nm).

PubMed

de Almeida, Leopoldina Fátima Dantas; Basso, Fernanda Gonçalves; Turrioni, Ana Paula Silveira; de-Souza-Costa, Carlos Alberto; Hebling, Josimeri

2016-01-01

Blue light emitting diodes (LEDs) are frequently used in dentistry for light activation of resin-based materials; however, their photobiostimulatory effects have not yet been fully investigated. This study aimed to investigate the effect of blue LED (455 nm) on the metabolism of odontoblast-like cells MDPC-23. Energy doses of 2 and 4 J/cm(2) were used at 20 mW/cm(2) fixed power density. MDPC-23 cells were seeded at 10,000 cells/cm(2) density in Dulbecco's modified Eagle's medium (DMEM) containing 10 % fetal bovine serum (FBS). After 12 h, the culture medium was replaced with new DMEM supplemented with 0.5 % of FBS, and the cells were incubated for further 12 h. After that, single irradiation was performed to the culture, under selected parameters. Cell viability evaluations (Alamar Blue Assay, n = 12), number of viable cells (Trypan Blue Assay, n = 12), morphological analysis by scanning electron microscopy (SEM, n = 2), gene expression (n = 6) of alkaline phosphatase (Alp), collagen (Col-1a1), and dental matrix protein (Dmp-1) (quantitative polymerase chain reaction (qPCR)) were performed 72 h after irradiation. Data were analyzed by Kruskal-Wallis, ANOVA, and Tukey tests (p < 0.05). Direct light application at 4 J/cm(2) energy dose had no negative effects on cell viability, while irradiation with 2 J/cm(2) reduced cell metabolism. None of doses affected the number of viable cells compared with the control group. The two energy doses downregulated the expression of Alp; however, expression of Col-1a1 and Dmp-1 had no alteration. Cells presented change in the cytoskeleton only when irradiated with 2 J/cm(2). In conclusion, the blue LED (455 nm) irradiation, under the evaluated parameters, had no biostimulatory effects on MDPC-23 cells.

Insulin stimulates synthesis and release of human chorionic gonadotropin by choriocarcinoma cell lines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ren, S.G.; Braunstein, G.D.

1991-03-01

Recent studies have shown that insulin regulates placental lactogen, progesterone, and estrogen production from human trophoblast cells. This study was performed to examine whether insulin also regulates the production of hCG by this type of cell. After 24-36 h of preincubation, JEG-3 and JAR cells (2-3 x 10(5) cells/ml.well) or human term trophoblast cells (1 x 10(6) cells/ml.well) were exposed to the test hormone in serum-free Dulbecco's Modified Eagle's Medium for 24-96 h. Secretion of hCG from JEG-3 cells was stimulated by human insulin, human proinsulin, or porcine insulin in a dose-dependent manner, with lowest effective doses of 6.7, 96,more » and 53 mg/L, respectively. Time-course studies showed that hCG secretion peaked at 72-96 h with insulin exposure; in contrast, no decernable peak was seen without insulin in serum-free media. Exposure of JEG-3 cells for 24 h to 209 mg/liter insulin stimulated hCG synthesis, with 40 +/- 3% more immunoreactive intracellular hCG (P less than 0.05). Cells grown in the presence of insulin and (35S)methionine had 47 +/- 21% more labeled intracellular hCG and 56 +/- 13% more immunoprecipitable (35S)methionine-hCG secreted into the medium than the control cultures (P less than 0.05). During this time period, human placental lactogen release and total trichloroacetice acid-precipitable (35S)methionine protein were not increased. The insulin-induced stimulation of hCG synthesis was inhibited by cycloheximide. Additionally, insulin did not significantly affect total intracellular protein during 24-96 h of incubation. Insulin also increased hCG release from JAR cells, but not from human term trophoblast cells. A mouse monoclonal antibody to the IGF-I receptor inhibited the stimulation of insulin in JEG-3 cells.« less

Mesenchymal Stem Cells Derived from Human Limbal Niche Cells

PubMed Central

Li, Gui-Gang; Zhu, Ying-Ting; Xie, Hua-Tao; Chen, Szu-Yu; Tseng, Scheffer C. G.

2012-01-01

Purpose. We investigated whether human limbal niche cells generate mesenchymal stem cells. Methods. Limbal niche cells were isolated from the limbal stroma by collagenase alone or following dispase removal of the limbal epithelium (D/C), and cultured on plastic in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), or coated or three-dimensional Matrigel in embryonic stem cell medium with leukemia inhibitory factor and basic fibroblast growth factor. Expression of cell markers, colony-forming units-fibroblast, tri-lineage differentiation, and ability of supporting limbal epithelial stem/progenitor cells were compared to limbal residual stromal cells. Results. Stromal cells expressing angiogenesis markers were found perivascularly, subjacent to limbal basal epithelial cells, and in D/C and limbal residual stromal cells. When seeded in three-dimensional Matrigel, D/C but not limbal residual stromal cells yielded spheres of angiogenesis progenitors that stabilized vascular networks. Similar to collagenase-isolated cells, D/C cells could be expanded on coated Matrigel for more than 12 passages, yielding spindle cells expressing angiogenesis and mesenchymal stem cells markers, and possessing significantly higher colony-forming units-fibroblast and more efficient tri-lineage differentiation than D/C and limbal residual stromal cells expanded on plastic in DMEM with 10% FBS, of which both lost the pericyte phenotype while limbal residual stromal cells turned into myofibroblasts. Upon reunion with limbal epithelial stem/progenitor cells to form spheres, D/C cells expanded on coated Matrigel maintained higher expression of p63α and lower expression of cytokeratin 12 than those expanded on plastic in DMEM with 10% FBS, while spheres formed with human corneal fibroblasts expressed cytokeratin 12 without p63α. Conclusions. In the limbal stroma, cells subjacent to limbal basal epithelial cells serve as niche cells, and generate progenitors with angiogenesis and mesenchymal stem cells potentials. They might partake in angiogenesis and regeneration during corneal wound healing. PMID:22836771

Nandrolone, an anabolic steroid, stabilizes Numb protein through inhibition of mdm2 in C2C12 myoblasts.

PubMed

Liu, Xin-Hua; Yao, Shen; Levine, Alice C; Kirschenbaum, Alexander; Pan, Jiangping; Wu, Yong; Qin, Weiping; Collier, Lauren; Bauman, William A; Cardozo, Christopher P

2012-01-01

Nandrolone, an anabolic steroid, slows denervation atrophy of rat muscle, prevents denervation-induced nuclear accumulation of intracellular domain of the Notch receptor, and elevates expression of Numb. Numb acts as an inhibitor of Notch signaling and promotes myogenic differentiation of satellite cells. Turnover of Numb is regulated by mdm2, an E3 ubiquitin ligase. With these considerations in mind, we investigated the effects of nandrolone on the expression of Numb and mdm2 proteins and determined the effect of mdm2 on nandrolone-induced alterations in Numb protein in C2C12 myoblasts. When C2C12 cells were cultured in a medium favoring differentiation (Dulbecco modified Eagle medium containing 2% horse serum), nandrolone up-regulated Numb protein levels in a time-dependent manner and prolonged Numb protein half-life from 10 to 18 hours. In contrast, nandrolone reduced the expression of mdm2 protein. To determine whether the decreased mdm2 expression induced by nandrolone was responsible for the increased levels and prolonged half-life of Numb protein in this cell line, mdm2-small interfering RNA (siRNA) was employed to inhibit mdm2 expression. Compared to cells transfected with scrambled siRNA (negative control), transfection with mdm2-siRNA increased basal Numb protein expression but abolished the further increase in Numb protein levels by nandrolone. In addition, transfection of mdm2-siRNA mimicked the effect of nandrolone to prolong the half-life of Numb protein. Moreover, when C2C12 cells were forced to overexpress mdm2, there was a significant decline in the expression of both basal and inducible Numb protein. Our data suggest that nandrolone, by a novel mechanism for this agent in a muscle cell type, increases Numb protein levels in C2C12 myoblasts by stabilizing Numb protein against degradation, at least in part, via suppression of mdm2 expression.

Far-infrared and dust properties of present-day galaxies in the EAGLE simulations

NASA Astrophysics Data System (ADS)

Camps, Peter; Trayford, James W.; Baes, Maarten; Theuns, Tom; Schaller, Matthieu; Schaye, Joop

2016-10-01

The Evolution and Assembly of GaLaxies and their Environments (EAGLE) cosmological simulations reproduce the observed galaxy stellar mass function and many galaxy properties. In this work, we study the dust-related properties of present-day EAGLE galaxies through mock observations in the far-infrared and submm wavelength ranges obtained with the 3D dust radiative transfer code SKIRT. To prepare an EAGLE galaxy for radiative transfer processing, we derive a diffuse dust distribution from the gas particles and we re-sample the star-forming gas particles and the youngest star particles into star-forming regions that are assigned dedicated emission templates. We select a set of redshift-zero EAGLE galaxies that matches the K-band luminosity distribution of the galaxies in the Herschel Reference Survey (HRS), a volume-limited sample of about 300 normal galaxies in the Local Universe. We find overall agreement of the EAGLE dust scaling relations with those observed in the HRS, such as the dust-to-stellar mass ratio versus stellar mass and versus NUV-r colour relations. A discrepancy in the f250/f350 versus f350/f500 submm colour-colour relation implies that part of the simulated dust is insufficiently heated, likely because of limitations in our sub-grid model for star-forming regions. We also investigate the effect of adjusting the metal-to-dust ratio and the covering factor of the photodissociation regions surrounding the star-forming cores. We are able to constrain the important dust-related parameters in our method, informing the calculation of dust attenuation for EAGLE galaxies in the UV and optical domain.

Using bald eagles to track spatial (1999-2008) and temporal (1987-1992, 1999-2003, and 2004-2008) trends of contaminants in Michigan's aquatic ecosystems.

PubMed

Wierda, Michael R; Leith, Katherine F; Roe, Amy S; Grubb, Teryl G; Sikarskie, James G; Best, David A; Pittman, H Tyler; Fuentes, Latice; Simon, Kendall L; Bowerman, William

2016-08-01

The bald eagle (Haliaeetus leucocephalus) is an extensively researched tertiary predator. Studies have delineated information about its life history and the influences of various stressors on its reproduction. Due to the bald eagle's position at the top of the food web, it is susceptible to biomagnification of xenobiotics. The Michigan Department of Environmental Quality implemented a program in 1999 to monitor persistent chemicals including polychlorinated biphenols (PCBs) and dichlorodiphenyltrichloroethane (DDE). The objectives of the present study were to evaluate spatial and temporal trends of PCBs and organochlorine pesticides in nestling bald eagles of Michigan. The authors' study found that concentrations of PCBs and DDE were higher in Great Lakes areas with Lakes Michigan and Lake Huron having the highest concentrations of DDE and Lake Erie having the highest concentrations of PCBs. Temporally (1987-1992, 1999-2003, and 2004-2008) the present study found declines in PCB and DDE concentrations with a few exceptions. Continued monitoring of Michigan bald eagle populations is suggested for a couple of reasons. First, nestling blood contaminant levels are an appropriate method to monitor ecosystem contaminant levels. Second, from 1999 to 2008 PCB and DDE concentrations for 30% and 40%, respectively, of the nestling eagles sampled were above the no observable adverse effect level (NOAEL) for bald eagles. Lastly, with the continued development and deployment of new chemistries a continuous long term monitoring program is an invaluable resource. Environ Toxicol Chem 2016;35:1995-2002. © 2016 SETAC. © 2016 SETAC.

Satellite tracking of two lesser spotted eagles Aquila pomarina, migrating from Namibia

USGS Publications Warehouse

Meyburg, B.-U.; Ellis, D.H.; Meyburg, C.; Mendelsohn, J.; Scheller, W.

2001-01-01

One immature and one subadult Lesser Spotted Eagle, Aquila pomarina, were followed by satellite telemetry from their non-breeding areas in Namibia. Both birds were fitted with transmitters (PTTs) in February 1994 and tracked, the immature for six months and two weeks, over distances of 10084 and 16773 km, respectively. During their time in Namibia both birds? movements were in response to good local rainfall. The immature eagle left Namibia at the end of February, the subadult at the end of March. They flew to their respective summer quarters in Hungary and the Ukraine, arriving there 2.5 and 1.5 months later than the breeding adults. The immature eagle took over two months longer on the homeward journey than a breeding male followed by telemetry in a previous study. On returning, the immature eagle followed the narrow flightpath through Africa used by other Lesser Spotted Eagles on their outward migration. It reached this corridor, which runs roughly between longitudes 31? and 36? East from Suez to Lake Tanganyika, veering from the shortest route in a direction east-northeast through Angola and Zambia to the southern end of Lake Tanganyika. The route taken by the subadult bird on its return migration differed markedly from that of all Lesser Spotted Eagles tracked to date, running further west through the Democratic Republic of Congo where, level with the equator, it flew over the eastern rainforest of that country. The outward migration, however, followed the same corridor and coincided in time with the migration of adults.

Movements by juvenile and immature Steller's Sea Eagles Haliaeetus pelagicus tracked by satellite

USGS Publications Warehouse

McGrady, M.J.; Ueta, M.; Potapov, E.R.; Utekhina, I.; Marterov, V.; Ladyguine, A.; Zykov, V.; Cibor, J.; Fuller, Mark R.; Seegar, J.K.

2003-01-01

Twenty-four juvenile Steller's Sea Eagles Haliaeetus pelagicus were tracked via satellite from natal areas in Magadan, Kabarovsk, Amur, Sakhalin and Kamchatka. Nestling dispersal occurred between 9 September and 6 December (n = 24), mostly 14 September-21 October, and did not differ among regions or years. Most eagles made stopovers of 4-28 days during migration. Migration occurred 9 September-18 January, mostly along previously described routes, taking 4-116 days to complete (n = 18). Eagles averaged 47.8 km/day excluding stopovers; 22.9 km/day including stopovers. The mean degrees of latitude spanned during migration was: Kamchatka, 2.1; Magadan, 11.6; Amur, 7.3; and Sakhalin, 1.1. Eagle winter range sizes varied. Eagles concentrated in 1-3 subareas within overall winter ranges. The mean size of the first wintering subareas was 274 km2, the second 529 km2, and the third 1181 km2. Second wintering areas were south of first wintering areas. Spring migration started between 2 February and 31 March. Two eagles from Magadan were tracked onto summering grounds, well south of their natal areas. Both had early and late summering areas. One bird was followed for 25 months. It initiated its second autumn migration in the first half of October and arrived on its wintering grounds on 26 December. The second autumn migration covered 1839 km (20.9-22.4 km/day). Unlike its first winter when it used two subareas, this bird used only one subarea in 1998-99, but this was located near wintering areas used in 1997-98. It left its wintering ground between 13 April and 13 May, and arrived on its summering grounds between 7 June and 8 July. Unlike most satellite radiotracking studies, data are presented from a relatively large number of birds from across their breeding range, including new information on eagle movements on the wintering grounds and during the second year

Factors that influence mercury concentrations in nestling Eagle Owls (Bubo bubo).

PubMed

Espín, Silvia; Martínez-López, Emma; León-Ortega, Mario; Calvo, José F; García-Fernández, Antonio Juan

2014-02-01

Mercury (Hg) is a global pollutant that bioaccumulates and biomagnifies in food chains, and is associated with adverse effects in both humans and wildlife. The Hg levels detected in blood obtained from Eagle Owl (Bubo bubo) chicks in Southeast Spain (Murcia) can be considered low (mean Hg concentration in blood from 2006 to 2012 was 36.83 ± 145.58 μg/l wet weigh, n=600), and it is therefore unlikely that Hg pollution can negatively affect their breeding. Positive correlation (r=0.339, p<0.001, n=229) was found to exist between the Hg concentrations in the blood and back feathers of the chicks. We provide a regression equation that could be helpful to estimate blood Hg levels when analyzing Hg concentrations in back feathers. Blood Hg concentrations in Eagle Owls have shown positive correlations with Hg levels in rabbit muscles, more evident in nests from the Northern area (r=0.600, p=0.014, n=16), where rabbits are the main prey of Eagle Owls. The best Linear Mixed Model to explain variations in blood Hg concentrations in nestling Eagle Owls includes year and location within the mining area as variables. The variable year is assigned the largest value of relative importance, followed by the location in the ancient mining sites and then the zone. Rainfalls may have an effect on the temporal differences in the blood Hg concentrations of nestling Eagle Owls. Although the studied region is not considered Hg polluted, the Hg levels were higher in Eagle Owls and European Rabbits from areas within the ancient mining sites as compared to those in the entire region. This result shows that spatial differences in Hg concentrations in Eagle Owls may be affected by local contamination, and that the role of diet composition may be less significant. © 2013.

Viability of human fibroblasts in coconut water as a storage medium.

PubMed

Moreira-Neto, J J S; Gondim, J O; Raddi, M S G; Pansani, C A

2009-09-01

To evaluate the effectiveness of a new storage medium for avulsed teeth, coconut water, in maintaining the viability of human fibroblasts. Cell viability after different time periods was evaluated in the following storage media: coconut water, coconut water with sodium bicarbonate, milk, saline and still mineral water. Human fibroblasts were seeded in Eagle's minimal essential medium (EMEM) supplemented with 7.5% foetal calf serum. After trypsinisation, 100 microL of culture medium containing approximately 10(4) cells mL(-1) were collected and pipetted into the wells of 96-well plates, which were incubated overnight in 5% CO(2) and 95% air mixture at 37 degrees C. EMEM was then replaced by the storage media and the plates were incubated at 37 degrees C for 1, 2 and 4 h. Cell viability was determined using the neutral red assay. The proportions of viable cells after exposure to the storage media were analysed statistically by anova and the least significant difference (LSD) test (alpha = 5%). Milk had the greatest capacity to maintain cell viability (P < 0.05), followed by coconut water with sodium bicarbonate and saline. Coconut water was significantly worse at maintaining cell viability compared to milk, coconut water with sodium bicarbonate and saline. The smallest number of viable cells was observed for mineral water (P < 0.05). Coconut water was worse than milk in maintaining human fibroblast cell viability.

Influence of cell culture medium composition on in vitro dissolution behavior of a fluoride-containing bioactive glass.

PubMed

Shah, Furqan A; Brauer, Delia S; Wilson, Rory M; Hill, Robert G; Hing, Karin A

2014-03-01

Bioactive glasses are used clinically for bone regeneration, and their bioactivity and cell compatibility are often characterized in vitro, using physiologically relevant test solutions. The aim of this study was to show the influence of varying medium characteristics (pH, composition, presence of proteins) on glass dissolution and apatite formation. The dissolution behavior of a fluoride-containing bioactive glass (BG) was investigated over a period of one week in Eagle's Minimal Essential Medium with Earle's Salts (MEM), supplemented with either, (a) acetate buffer, (b) 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, (c) HEPES + carbonate, or (d) HEPES + carbonate + fetal bovine serum. Results show pronounced differences in pH, ion release, and apatite formation over 1 week: Despite its acidic pH (pH 5.8 after BG immersion, as compared to pH 7.4-8.3 for HEPES-containing media), apatite formation was fastest in acetate buffered (HEPES-free) MEM. Presence of carbonate resulted in formation of calcite (calcium carbonate). Presence of serum proteins, on the other hand, delayed apatite formation significantly. These results confirm that the composition and properties of a tissue culture medium are important factors during in vitro experiments and need to be taken into consideration when interpreting results from dissolution or cell culture studies. Copyright © 2013 Wiley Periodicals, Inc.

An efficient recovery method for enteric viral particles from agricultural soils.

PubMed

Brassard, Julie; Gagné, Marie-Josée

2018-06-24

Enteric viruses have been recognized as the leading cause of non-bacterial gastroenteritis and hepatitis outbreaks around the world. Understanding their prevalence and persistence in the environment is important for the effective control of these infections. The aim of this study was to develop an efficient recovery procedure for viral infectious particles from agricultural soils. Samples (25 g) of soil (black earth soil, loamy soil, and sandy soil) were spiked with murine norovirus (MNV) and feline calicivirus (FCV), mixed with five different buffers and viral genetic material was extracted by 3 commercial kits. The combination consisted by the modified Eagle's medium buffer followed by Dynabeads nucleic acid extraction kit, when the detection is conducted by molecular biology, has been identified as being the most effective procedure to preserve the viral particle infectivity and also to remove PCR inhibitors.The recovery percentages of infectious MNV for the 3 types of soils were 54.3%, 54.4%, and 56.9%. In contrast, the titres of the FCV varied depending on the type of soil, and the recovery percentages were 47.8% in the black soil, 15.6% in the loamy soil, and 17.7% in the sandy soil. Also, the results presented in this study highlight the importance of using an internal process control such as artificial inoculation with MNV at known concentrations during detection by molecular methods, in order to avoid the occurrence of false negative reactions. Copyright © 2018. Published by Elsevier B.V.

In Vitro Cytotoxicity Assessment of an Orthodontic Composite Containing Titanium-dioxide Nano-particles.

PubMed

Heravi, Farzin; Ramezani, Mohammad; Poosti, Maryam; Hosseini, Mohsen; Shajiei, Arezoo; Ahrari, Farzaneh

2013-01-01

Background and aims. Incorporation of nano-particles to orthodontic bonding systems has been considered to prevent enamel demineralization around appliances. This study investigated cytotoxicity of Transbond XT adhesive containing 1 wt% titanium dioxide (TiO2) nano-particles. Materials and methods. Ten composite disks were prepared from each of the conventional and TiO2-containg composites and aged for 1, 3, 5, 7 and 14 days in Dulbecco's Modified Eagle's Medium (DMEM). The extracts were obtained and exposed to culture media of human gingival fibroblasts (HGF) and mouse L929 fibroblasts. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results. Both adhesives were moderately toxic for HGF cells on the first day of the experiment, but the TiO2-containing adhesive produced significantly lower toxicity than the pure adhesive (P<0.05). No significant differences were found in cell viability percentages between the two groups on the other days (P>0.05). There was a significant reduction in cell toxicity with increasing pre-incubation time (P<0.001). L929 cells showed similar toxicity trends, but lower sensitivity to detect cytotoxicity of dental composites. Conclusion. The orthodontic adhesive containing TiO2 nano-particles indicated comparable or even lower toxicity than its nano-particle-free counterpart, indicating that incorporation of 1 wt% TiO2 nano-particles to the composite structure does not result in additional health hazards compared to that occurring with the pure adhesive.

Identification of adequate vehicles to carry nerve regeneration inducers using tubulisation.

PubMed

do Nascimento-Elias, Adriana Helena; Fresnesdas, Bruno César; Schiavoni, Maria Cristina Lopes; de Almeida, Natália Fernanda Gaspar; Santos, Ana Paula; de Oliveira Ramos, Jean; Junior, Wilson Marques; Barreira, Amilton Antunes

2012-08-14

Axonal regeneration depends on many factors, such as the type of injury and repair, age, distance from the cell body and distance of the denervated muscle, loss of surrounding tissue and the type of injured nerve. Experimental models use tubulisation with a silicone tube to research regenerative factors and substances to induce regeneration. Agarose, collagen and DMEM (Dulbecco's modified Eagle's medium) can be used as vehicles. In this study, we compared the ability of these vehicles to induce rat sciatic nerve regeneration with the intent of finding the least active or inert substance. The experiment used 47 female Wistar rats, which were divided into four experimental groups (agarose 4%, agarose 0.4%, collagen, DMEM) and one normal control group. The right sciatic nerve was exposed, and an incision was made that created a 10 mm gap between the distal and proximal stumps. A silicone tube was grafted onto each stump, and the tubes were filled with the respective media. After 70 days, the sciatic nerve was removed. We evaluated the formation of a regeneration cable, nerve fibre growth, and the functional viability of the regenerated fibres. Comparison among the three vehicles showed that 0.4% agarose gels had almost no effect on provoking the regeneration of peripheral nerves and that 4% agarose gels completely prevented fibre growth. The others substances were associated with profuse nerve fibre growth. In the appropriate concentration, agarose gel may be an important vehicle for testing factors that induce regeneration without interfering with nerve growth.

In vitro study of the adverse effect of nicotine and physical strain on human gingival fibroblasts as a model of the healing of wounds commonly found in the military.

PubMed

Dinos, Michael E; Borke, James L; Swiec, Gary D; McPherson, James C; Goodin, Jeremy L; Chuang, Augustine H

2015-03-01

Significant adverse effects on fibroblast growth and metabolism are observed with nicotine. We investigated the synergistic effects of nicotine and cyclical mechanical strain (CMS) on human gingival fibroblasts (HGFs) in a wound-healing model. HGFs were isolated and grown in Dulbecco's modified Eagle's medium. Three-millimeter wounds were created on a confluent cell monolayer grown in a media containing 0, 1, 2, or 4 mM nicotine, with or without CMS. The applied deformation regimen remains constant for 6 days. On days 1, 2, 4, and 6, the cells were stained with hematoxylin and eosin Y for the evaluation of wound repopulation. The application of CMS alone demonstrates a biphasic response, with an initial stimulatory effect on wound repopulation (days 1-2) and less repopulation during the later phase (days 4-6). The addition of nicotine clearly demonstrated a time and inverse dose-dependent relationship on wound repopulation, with no effect during the early phase and reduced wound repopulation during the later phase. Initial treatment of HGF wounds with CMS resulted in faster wound repopulation regardless of nicotine presence. By day 6, wound healing of HGF exposed to both nicotine and CMS is delayed. These findings suggest that CMS and nicotine may affect fibroblasts and delay wound healing at other sites in the body as well. Reprint & Copyright © 2015 Association of Military Surgeons of the U.S.

In vitro cytotoxicity of maxillofacial silicone elastomers: effect of accelerated aging.

PubMed

Bal, Bilge Turhan; Yilmaz, Handan; Aydin, Cemal; Karakoca, Seçil; Yilmaz, Sükran

2009-04-01

The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L-929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc-shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05).

Warrior Model for Human Performance and Injury Prevention: Eagle Tactical Athlete Program (ETAP) Part II.

PubMed

Sell, Timothy C; Abt, John P; Crawford, Kim; Lovalekar, Mita; Nagai, Takashi; Deluzio, Jennifer B; Smalley, Brain W; McGrail, Mark A; Rowe, Russell S; Cardin, Sylvain; Lephart, Scott M

2010-01-01

Physical training for United States military personnel requires a combination of injury prevention and performance optimization to counter unintentional musculoskeletal injuries and maximize warrior capabilities. Determining the most effective activities and tasks to meet these goals requires a systematic, research-based approach that is population specific based on the tasks and demands of the Warrior. The authors have modified the traditional approach to injury prevention to implement a comprehensive injury prevention and performance optimization research program with the 101st Airborne Division (Air Assault) at Fort Campbell, KY. This is second of two companion papers and presents the last three steps of the research model and includes Design and Validation of the Interventions, Program Integration and Implementation, and Monitor and Determine the Effectiveness of the Program. An 8-week trial was performed to validate the Eagle Tactical Athlete Program (ETAP) to improve modifiable suboptimal characteristics identified in Part I. The experimental group participated in ETAP under the direction of a ETAP Strength and Conditioning Specialist while the control group performed the current physical training at Fort Campbell under the direction of a Physical Training Leader and as governed by FM 21-20 for the 8-week study period. Soldiers performing ETAP demonstrated improvements in several tests for strength, flexibility, performance, physiology, and the APFT compared to current physical training performed at Fort Campbell. ETAP was proven valid to improve certain suboptimal characteristics within the 8-week trial as compared to the current training performed at Fort Campbell. ETAP has long-term implications and with expected greater improvements when implemented into a Division pre-deployment cycle of 10-12 months which will result in further systemic adaptations for each variable.

Direct Demonstration of the Concept of Unrestricted Effective-Medium Approximation

NASA Technical Reports Server (NTRS)

Mishchenko, Michael I.; Dlugach, Zhanna M.; Zakharova, Nadezhda T.

2014-01-01

The modified unrestricted effective-medium refractive index is defined as one that yields accurate values of a representative set of far-field scattering characteristics (including the scattering matrix) for an object made of randomly heterogeneous materials. We validate the concept of the modified unrestricted effective-medium refractive index by comparing numerically exact superposition T-matrix results for a spherical host randomly filled with a large number of identical small inclusions and Lorenz-Mie results for a homogeneous spherical counterpart. A remarkable quantitative agreement between the superposition T-matrix and Lorenz-Mie scattering matrices over the entire range of scattering angles demonstrates unequivocally that the modified unrestricted effective-medium refractive index is a sound (albeit still phenomenological) concept provided that the size parameter of the inclusions is sufficiently small and their number is sufficiently large. Furthermore, it appears that in cases when the concept of the modified unrestricted effective-medium refractive index works, its actual value is close to that predicted by the Maxwell-Garnett mixing rule.

Double-survey estimates of bald eagle populations in Oregon

USGS Publications Warehouse

Anthony, R.G.; Garrett, Monte G.; Isaacs, F.B.

1999-01-01

The literature on abundance of birds of prey is almost devoid of population estimates with statistical rigor. Therefore, we surveyed bald eagle (Haliaeetus leucocephalus) populations on the Crooked and lower Columbia rivers of Oregon and used the double-survey method to estimate populations and sighting probabilities for different survey methods (aerial, boat, vehicle) and bald eagle ages (adults vs. subadults). Sighting probabilities were consistently 20%. The results revealed variable and negative bias (percent relative bias = -9 to -70%) of direct counts and emphasized the importance of estimating populations where some measure of precision and ability to conduct inference tests are available. We recommend use of the double-survey method to estimate abundance of bald eagle populations and other raptors in open habitats.

Bald eagles nesting in Baja California

USGS Publications Warehouse

Henny, Charles J.; Anderson, Daniel W.; Knoder, C.E.

1978-01-01

Published records of Bald Eagles (Haliaeetus leucocephalus) nesting in Baja California during the last 50 years are nonexistent to our knowledge, and few records exist prior to that time. Friedmann et al. (1950:61, Pac. Coast Avifauna 29) describe the distribution of Bald Eagles in Baja California as "a scattering of pairs on both the Pacific and Gulf Sides." Nesting Bald Eagles were first reported by Bryant (1889, Proc. Calif. Acad. Sci. 2: 237-320), who found a pair on Isla Santa Margarita (24°25'N, 111°50'W; hereafter abbreviated as 2425-11150) and saw an adult on the "estero" north from Magdalena Bay (the region where one pair was seen in 1977). Other records were reviewed by Grinnell (1928, Univ. Calif. Publ. Zool. 32).

Corrosion resistance, mechanical properties, corrosion fatigue strength and cytocompatibility of new Ti alloys without Al and V.

PubMed

Okazaki, Y; Rao, S; Ito, Y; Tateishi, T

1998-07-01

The effects of various metallic ions using various metallic powders on the relative growth ratio of fibroblasts L929 and osteoblasts MC3T3-E1 cells were carried out. Ti, Zr, Sn, Nb and Ta had evidently no effect on the relative growth ratios of cells. Otherwise, Al and V ions exhibit cytotoxicity from a concentration of > or = 0.2 ppm. This Al effect on cells tend to be stronger in medium containing small quantity of V ions (< or = 0.03 ppm). The new Ti-15%Zr-4%Nb-4%Ta-0.2%Pd alloy exhibited a higher corrosion resistance in physiological saline solution. The addition of 0.02%O and 0.05%N to Ti-Zr alloy improved the mechanical properties at room temperature and corrosion fatigue strength. The relative growth ratios for the new Ti alloy plate and the alloy block extraction were unity. Further, the relative growth ratios were almost unity for the new Ti alloy against apatite ceramic pins up to 10(5) wear cycles in Eagle's MEM solution. However, there was a sharp decrease for Ti-6%Al-4%V ELI alloy from 3 x 10(4) wear cycles as V ion was released during wear into the wear test solution since the pH of the Eagle's MEM increases with increasing wear cycles.

Optimization of the Synthesis of Structured Phosphatidylcholine with Medium Chain Fatty Acid.

PubMed

Ochoa-Flores, Angélica A; Hernández-Becerra, Josafat A; Cavazos-Garduño, Adriana; Vernon-Carter, Eduardo J; García, Hugo S

2017-11-01

Structured phosphatidylcholine was successfully produced by acidolysis between phosphatidylcholine and free medium chain fatty acid, using phospholipase A 1 immobilized on Duolite A568. Response surface methodology was applied to optimize the reaction system using three process parameters: molar ratio of substrates (phosphatidylcholine to free medium chain fatty acid), enzyme loading, and reaction temperature. All parameters evaluated showed linear and quadratic significant effects on the production of modified phosphatidylcholine; molar ratio of substrates contributed positively, but temperature influenced negatively. Increased enzyme loading also led to increased production of modified phosphatidylcholine but only during the first 9 hours of the acidolysis reaction. Optimal conditions obtained from the model were a ratio of phosphatidylcholine to free medium chain fatty acid of 1:15, an enzyme loading of 12%, and a temperature of 45°C. Under these conditions a production of modified phosphatidylcholine of 52.98 % were obtained after 24 h of reaction. The prediction was confirmed from the verification experiments; the production of modified phosphatidylcholine was 53.02%, the total yield of phosphatidylcholine 64.28% and the molar incorporation of medium chain fatty acid was 42.31%. The acidolysis reaction was scaled-up in a batch reactor with a similar production of modified phosphatidylcholine, total yield of phosphatidylcholine and molar incorporation of medium chain fatty acid. Purification by column chromatography of the structured phosphatidylcholine yielded 62.53% of phosphatidylcholine enriched with 42.52% of medium chain fatty acid.

Ranging behaviour and habitat preferences of the Martial Eagle: Implications for the conservation of a declining apex predator

PubMed Central

van Eeden, Rowen; Whitfield, D. Philip; Botha, Andre; Amar, Arjun

2017-01-01

Understanding the ranging behaviours of species can be helpful in effective conservation planning. However, for many species that are rare, occur at low densities, or occupy challenging environments, this information is often lacking. The Martial Eagle (Polemaetus bellicosus) is a low density apex predator declining in both non-protected and protected areas in southern Africa, and little is known about its ranging behaviour. We use GPS tags fitted to Martial Eagles (n = 8) in Kruger National Park (KNP), South Africa to describe their ranging behaviour and habitat preference. This represents the first time that such movements have been quantified in adult Martial Eagles. Territorial eagles (n = 6) held home ranges averaging ca. 108 km2. Home range estimates were similar to expectations based on inter-nest distances, and these large home range sizes could constrain the carrying capacity of even the largest conservation areas. Two tagged individuals classed as adults on plumage apparently did not hold a territory, and accordingly ranged more widely (ca. 44,000 km2), and beyond KNP boundaries as floaters. Another two territorial individuals abandoned their territories and joined the ‘floater’ population, and so ranged widely after leaving their territories. These unexpected movements after territory abandonment could indicate underlying environmental degradation. Relatively high mortality of these wide-ranging ‘floaters’ due to anthropogenic causes (three of four) raises further concerns for the species’ persistence. Habitat preference models suggested Martial Eagles used areas preferentially that were closer to rivers, had higher tree cover, and were classed as dense bush rather than open bush or grassland. These results can be used by conservation managers to help guide actions to preserve breeding Martial Eagles at an appropriate spatial scale. PMID:28306744

Summer and winter space use and home range characteristics of Golden Eagles (Aquila chrysaetos) in eastern North America

USGS Publications Warehouse

Miller, Tricia A.; Brooks, Robert P.; Lanzone, Michael J.; Cooper, Jeff; O'Malley, Kieran; Brandes, David; Duerr, Adam E.; Katzner, Todd

2017-01-01

Movement behavior and its relationship to habitat provide critical information toward understanding the effects of changing environments on birds. The eastern North American population of Golden Eagles (Aquila chrysaetos) is a genetically distinct and small population of conservation concern. To evaluate the potential responses of this population to changing landscapes, we calculated the home range and core area sizes of 52 eagles of 6 age–sex classes during the summer and winter seasons. Variability in range size was related to variation in topography and open cover, and to age and sex. In summer, eagle ranges that were smaller had higher proportions of ridge tops and open cover and had greater topographic roughness than did larger ranges. In winter, smaller ranges had higher proportions of ridge tops, hillsides and cliffs, and open cover than did larger ranges. All age and sex classes responded similarly to topography and open cover in both seasons. Not surprisingly, adult eagles occupied the smallest ranges in both seasons. Young birds used larger ranges than adults, and subadults in summer used the largest ranges (>9,000 km2). Eastern adult home ranges in summer were 2–10 times larger than those reported for other populations in any season. Golden Eagles in eastern North America may need to compensate for generally lower-quality habitat in the region by using larger ranges that support access to adequate quantities of resources (prey, updrafts, and nesting, perching, and roosting sites) associated with open cover and diverse topography. Our results suggest that climate change–induced afforestation on the breeding grounds and ongoing land cover change from timber harvest and energy development on the wintering grounds may affect the amount of suitable habitat for Golden Eagles in eastern North America.

Spatial demographic models to inform conservation planning of golden eagles in renewable energy landscapes

USGS Publications Warehouse

Wiens, J. David; Schumaker, Nathan H.; Inman, Richard D.; Esque, Todd C.; Longshore, Kathleen M.; Nussear, Kenneth E

2017-01-01

Spatial demographic models can help guide monitoring and management activities targeting at-risk species, even in cases where baseline data are lacking. Here, we provide an example of how site-specific changes in land use and anthropogenic stressors can be incorporated into a spatial demographic model to investigate effects on population dynamics of Golden Eagles (Aquila chrysaetos). Our study focused on a population of Golden Eagles exposed to risks associated with rapid increases in renewable energy development in southern California, U.S.A. We developed a spatially explicit, individual-based simulation model that integrated empirical data on demography of Golden Eagles with spatial data on the arrangement of nesting habitats, prey resources, and planned renewable energy development sites. Our model permitted simulated eagles of different stage-classes to disperse, establish home ranges, acquire prey resources, prospect for breeding sites, and reproduce. The distribution of nesting habitats, prey resources, and threats within each individual's home range influenced movement, reproduction, and survival. We used our model to explore potential effects of alternative disturbance scenarios, and proposed conservation strategies, on the future distribution and abundance of Golden Eagles in the study region. Results from our simulations suggest that probable increases in mortality associated with renewable energy infrastructure (e.g., collisions with wind turbines and vehicles, electrocution on power poles) could have negative consequences for population trajectories, but that site-specific conservation actions could reduce the magnitude of negative effects. Our study demonstrates the use of a flexible and expandable modeling framework to incorporate spatially dependent processes when determining relative effects of proposed management options to Golden Eagles and their habitats.

Laying the Foundations for a Human-Predator Conflict Solution: Assessing the Impact of Bonelli's Eagle on Rabbits and Partridges

PubMed Central

Moleón, Marcos; Sánchez-Zapata, José A.; Gil-Sánchez, José M.; Barea-Azcón, José M.; Ballesteros-Duperón, Elena; Virgós, Emilio

2011-01-01

Background Predation may potentially lead to negative effects on both prey (directly via predators) and predators (indirectly via human persecution). Predation pressure studies are, therefore, of major interest in the fields of theoretical knowledge and conservation of prey or predator species, with wide ramifications and profound implications in human-wildlife conflicts. However, detailed works on this issue in highly valuable –in conservation terms– Mediterranean ecosystems are virtually absent. This paper explores the predator-hunting conflict by examining a paradigmatic, Mediterranean-wide (endangered) predator-two prey (small game) system. Methodology/Principal Findings We estimated the predation impact (‘kill rate’ and ‘predation rate’, i.e., number of prey and proportion of the prey population eaten, respectively) of Bonelli's eagle Aquila fasciata on rabbit Oryctolagus cuniculus and red-legged partridge Alectoris rufa populations in two seasons (the eagle's breeding and non-breeding periods, 100 days each) in SE Spain. The mean estimated kill rate by the seven eagle reproductive units in the study area was c. 304 rabbits and c. 262 partridges in the breeding season, and c. 237 rabbits and c. 121 partridges in the non-breeding period. This resulted in very low predation rates (range: 0.3–2.5%) for both prey and seasons. Conclusions/Significance The potential role of Bonelli's eagles as a limiting factor for rabbits and partridges at the population scale was very poor. The conflict between game profitability and conservation interest of either prey or predators is apparently very localised, and eagles, quarry species and game interests seem compatible in most of the study area. Currently, both the persecution and negative perception of Bonelli's eagle (the ‘partridge-eating eagle’ in Spanish) have a null theoretical basis in most of this area. PMID:21818399

Satellite tracking of two Lesser Spotted Eagles, Aquila pomarina, migrating from Namibia

USGS Publications Warehouse

Meyburg, B.-U.; Ellis, D.H.; Meyburg, C.; Mendelsohn, J.M.; Scheller, W.

2001-01-01

One immature and one subadult Lesser Spotted Eagle, Aquila pomarina, were followed by satellite telemetry from their nonbreeding areas in Namibia. Both birds were fitted with transmitters (PTTs) in February 1994 and tracked, the immature for six months and three weeks, the subadult for eight months and two weeks, over distances of 10 084 and 16 773 km, respectively. During their time in Namibia both birds' movements were in response to good local rainfall. The immature eagle left Namibia at the end of February, the subadult at the end of March. They flew to their respective summer quarters in Hungary and the Ukraine, arriving there 2.5 and 1.5 months later than the breeding adults. The immature eagle took over two months longer on the homeward journey than a breeding male followed by telemetry in a previous study. On returning, the immature eagle followed the narrow flightpath through Africa used by other Lesser Spotted Eagles on their outward migration. It reached this corridor, which runs roughly between longitudes 31?? and 36?? East from Suez to Lake Tanganyika, veering from the shortest route in a direction east-northeast through Angola and Zambia to the southern end of Lake Tanganyika. The route taken by the subadult bird on its return migration differed markedly from that of all Lesser Spotted Eagles tracked to date, running further west through the Democratic Republic of Congo where, level with the equator, it flew over the eastern rainforest of that country. The outward migration, however, followed the same corridor and coincided in time with the migration of adults. [A German translation of the abstract is provided on p. 40.].

EAGLE can do Efficient LTL Monitoring

NASA Technical Reports Server (NTRS)

Barringer, Howard; Goldberg, Allen; Havelund, Klaus; Sen, Koushik

2003-01-01

We briefly present a rule-based framework, called EAGLE, that has been shown to be capable of defining and implementing finite trace monitoring logics, including future and past time temporal logic, extended regular expressions, real-time logics, interval logics, forms of quantified temporal logics, and so on. In this paper we show how EAGLE can do linear temporal logic (LTL) monitoring in an efficient way. We give an upper bound on the space and time complexity of this monitoring.

Functions of perch relocations in a communal night roost of wintering bald eagles

USGS Publications Warehouse

Yackel Adams, A.A.; Skagen, S.K.; Knight, R.L.

2000-01-01

We investigated the functions of perch relocations within a communal night roost of wintering bald eagles (Haliaeetus leucocephalus) along the Nooksack River, Washington, during two winters. We tested seven predictions of two nonexclusive hypotheses: (1) bald eagles relocate within roosts to assess foraging success of conspecifics and (2) bald eagles relocate to obtain thermoregulatory benefits from an improved microclimate. Additionally, we gathered descriptive information to allow refinement of further alternative hypotheses. We rejected the hypothesis that relocations are a means of assessing foraging success. Contrary to our expectations, immature eagles did not relocate to be closer to adults, and relocations were less frequent when food was less abundant. Our data support the hypothesis that eagles relocate within night roosts to obtain a favorable microclimate during winters when they are subjected to cold stress and food stress. In both winters, relocations were more frequent in the evening than in the morning. In both winters, most evening relocations were to the center of the roost rather than to its edge, and the frequency of relocation to the center was greater when temperatures were low. The microclimate hypothesis, however, explains only a limited number of relocations. Based on our findings, it is likely that relocation has multiple functions, including establishing and (or) maintaining foraging associations, establishing and (or) maintaining social-dominance hierarchies when food is less abundant, and nonsocial activities.

Bald eagles view their territory from high tower at KSC

NASA Technical Reports Server (NTRS)

2000-01-01

A pair of Florida bald eagles take advantage of a tower to rest and view the landscape near the intersection of the NASA Causeway and Kennedy Parkway North at Kennedy Space Center. This pair of eagles nests near Kennedy Parkway and is seen frequently by KSC commuters and visitors. The Southern Bald Eagle ranges throughout Florida and along the coasts of California, Texas, Louisiana and the south Atlantic states. Bald Eagles are listed as endangered in the U.S., except in five states where they are listed as threatened. The number of nesting pairs of the southern race once numbered several thousand; recent estimates are only 350-375. Most southern Florida eagles nesting at KSC arrive during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. . Kennedy Space Center shares a boundary with the Merritt Island National Wildlife Refuge, which encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

A Population Study of Golden Eagles in the Altamont Pass Wind Resource Area: Population Trend Analysis, 1994-1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunt, W. G.; Jackman, R. E.; Hunt, T. L.

1999-07-20

The wind industry has annually reported 28-43 turbine blade strike casualties of golden eagles in the Altamont Pass Wind Resource Area, and many more carcasses have doubtless gone unnoticed. Because this species is especially sensitive to adult survival rate changes, we focused upon estimating the demographic trend of the population. In aerial surveys, we monitored survival within a sample of 179 radio-tagged eagles over a four-year period. We also obtained data on territory occupancy and reproduction of about 65 eagle pairs residing in the area. Of 61 recorded deaths of radio-tagged eagles during the four-year investigation, 23 (38%) were causedmore » by wind turbine blade strikes. Additional fatalities were unrecorded because blade strikes sometimes destroy radio transmitters. Annual survival was estimated at 0.7867 (SE=0.0263) for non-territorial eagles and 0.8964 (SE=0.0371) for territorial ones. Annual reproduction was 0.64 (SE=0.08) young per territorial pair (0.25 per female). These parameters were used to estimate population growth rates under different modeling frameworks. At present, there are indications that a reserve of non-breeding adults still exists, i.e., there is an annual territorial reoccupancy rate of 100% and a low incidence (3%) of subadults as members of breeding pairs.« less

Reference intervals, longitudinal analyses, and index of individuality of commonly measured laboratory variables in captive bald eagles (Haliaeetus leucocephalus).

PubMed

Jones, Michael P; Arheart, Kristopher L; Cray, Carolyn

2014-06-01

The objectives of this study were to determine reference intervals, perform longitudinal analyses, and determine the index of individuality (IoI) of 8 hematologic, and 13 biochemical and electrophoretic variables for a group of captive bald eagles (Haliaeetus leucocephalus). Reference intervals were determined from blood samples collected during annual wellness examinations for 41 eagles (23 male and 18 female) with ages ranging between 6 and 43 years (18.7 +/- 7.4, mean +/- SD) at the time of sample collection. Longitudinal analyses and IoI were determined for measured hematologic, biochemical, and protein electrophoretic variables, both individually and as a group, for a subset of 16 eagles (10 male and 6 female) during a 12-year period. This smaller group of eagles ranged in age between 2 and 20 years at the start of the study period, and between 14 and 32 years (21.9 +/- 5.0, mean +/- SD) at the end of the study period. Significant increases with age within the group of 16 eagles were observed only for red blood cells, percent heterophils, total protein, and beta-globulin protein fraction, while albumin:globulin decreased significantly with age. A low IoI (> or = 1.4) was determined for all hematologic and biochemical variables except gamma globulins, which had high IoI (< or = 0.6) for 3 individuals within the subset of 16.

Genetic structure and viability selection in the golden eagle (Aquila chrysaetos), a vagile raptor with a Holarctic distribution

USGS Publications Warehouse

Doyle, Jacqueline M.; Katzner, Todd E.; Roemer, Gary; Cain, James W.; Millsap, Brian; McIntyre, Carol; Sonsthagen, Sarah A.; Fernandez, Nadia B.; Wheeler, Maria; Bulut, Zafer; Bloom, Peter; DeWoody, J. Andrew

2016-01-01

Molecular markers can reveal interesting aspects of organismal ecology and evolution, especially when surveyed in rare or elusive species. Herein, we provide a preliminary assessment of golden eagle (Aquila chrysaetos) population structure in North America using novel single nucleotide polymorphisms (SNPs). These SNPs included one molecular sexing marker, two mitochondrial markers, 85 putatively neutral markers that were derived from noncoding regions within large intergenic intervals, and 74 putatively nonneutral markers found in or very near protein-coding genes. We genotyped 523 eagle samples at these 162 SNPs and quantified genotyping error rates and variability at each marker. Our samples corresponded to 344 individual golden eagles as assessed by unique multilocus genotypes. Observed heterozygosity of known adults was significantly higher than of chicks, as was the number of heterozygous loci, indicating that mean zygosity measured across all 159 autosomal markers was an indicator of fitness as it is associated with eagle survival to adulthood. Finally, we used chick samples of known provenance to test for population differentiation across portions of North America and found pronounced structure among geographic sampling sites. These data indicate that cryptic genetic population structure is likely widespread in the golden eagle gene pool, and that extensive field sampling and genotyping will be required to more clearly delineate management units within North America and elsewhere.

Bald eagle winter roost characteristics in Lava Beds National Monument, California

USGS Publications Warehouse

Stohlgren, Thomas J.

1993-01-01

This study provided a survey of bald eagle (Haliaeetus leucocephalus) winter roost habitat (in 4 km2 of potential roost areas) in southern Lava Beds National Monument, California. A systematic-clustered sampling design (n=381 plots) was used to compare forest stand characteristics in two primary roost areas (Caldwell Butte and Eagle Nest Butte) and two potential roost areas (Hidden Valley and Island Butte). A 100 percent inventory of roost trees in Caldwell Butte (n=103 trees) and Eagle Nest Butte (n=44 trees) showed they were spatially clumped and restricted to 12.7 percent and 2.8 percent, respectively, of the study areas. Roost trees, primarily ponderosa pine (Pinus ponderosa), averaged 81.1 ± 1.3 cm dbh (mean ± 1 S.E.) compared to non-roost trees (>35 cm dbh) that averaged 52.2 ± 1.0 cm dbh. Roost trees were generally taller and more open-structured than non-roost trees. All four study sites had adequate numbers of mid-sized trees (10 to 50 cm dbh) to replace the current stock of older, larger roost trees. However, seedling and small trees (<10 cm dbh) in the roost areas were spatially clumped and few, suggesting that maintaining a continuous population of roost trees may be a problem in the distant future. Long-term studies of changing winter roost habitat and eagle use are essential to protect the bald eagle in the northwestern US.

Monitoring bald eagles using lists of nests: Response to Watts and Duerr

USGS Publications Warehouse

Sauer, John R.; Otto, Mark C.; Kendall, William L.; Zimmerman, Guthrie S.

2011-01-01

The post-delisting monitoring plan for bald eagles (Haliaeetus leucocephalus) roposed use of a dual-frame sample design, in which sampling of known nest sites in combination with additional area-based sampling is used to estimate total number of nesting bald eagle pairs. Watts and Duerr (2010) used data from repeated observations of bald eagle nests in Virginia, USA to estimate a nest turnover rate and used this rate to simulate decline in number of occupied nests in list nests over time. Results of Watts and Duerr suggest that, given the rates of loss of nests from the list of known nest sites in Virginia, the list information will be of little value to sampling unless lists are constantly updated. Those authors criticize the plan for not placing sufficient emphasis on updating and maintaining lists of bald eagle nests. Watts and Duerr's metric of turnover rate does not distinguish detectability or temporary nonuse of nests from permanent loss of nests and likely overestimates turnover rate. We describe a multi-state capture–recapture model that allows appropriate estimation of rates of loss of nests, and we use the model to estimate rates of loss from a sample of nests from Maine, USA. The post-delisting monitoring plan addresses the need to maintain and update the lists of nests, and we show that dual frame sampling is an effective approach for sampling nesting bald eagle populations.

Anesthesia with isoflurane and sevoflurane in the crested serpent eagle (Spilornis cheela hoya): minimum anesthetic concentration, physiological effects, hematocrit, plasma chemistry and behavioral effects.

PubMed

Chan, Fang-Tse; Chang, Geng-Ruei; Wang, Hsien-Chi; Hsu, Tien-Huan

2013-12-30

The initial goal of this study was to determine the minimum anesthetic concentration (MAC) for isoflurane (ISO) and sevoflurane (SEVO) for the crested serpent eagle. Next, we compared the anesthetic effects of each on the physiological effects, hematocrit, plasma chemistry values and behavior in spontaneously breathing captive adult crested serpent eagles. Sixteen eagles were randomly allocated to two groups for anesthesia with ISO (n=8) or SEVO (n=8). First, we measured the MAC values of ISO and SEVO, and four weeks later, we investigated the effect of each on the physiological effects, hematocrit (HCT) and plasma chemistry values. The MAC values of ISO and SEVO for crested serpent eagles were 1.46 ± 0.30 and 2.03 ± 0.32%, respectively. The results revealed no significant differences between the two anesthetics in induction time, while time of extubation to recovery was significantly shorter with SEVO. A time-related increase in end-tidal CO₂ and decreases in body temperature and respiratory rates were observed during anesthesia with each anesthetic. There were no significant differences between the effect of the two anesthetics on heart rate, hematocrit, plasma chemistry values or respiration, although each caused minor respiration depression. We concluded that SEVO is a more effective inhalant agent than ISO for use in eagles, showing the most rapidest induction and recovery from anesthesia.

Assessing cumulative impacts to wintering Bald Eagles and their habitats in western Washington

DOE Office of Scientific and Technical Information (OSTI.GOV)

Witmer, G.W.; O'Neil, T.A.

Bald Eagles (Haliaeetus leucocephalus) of Washington, the largest wintering population in the lower 48 states, are subject to numerous pressures and impacts from human activites. An evaluative method potential cumulative impacts of multiple hydroelectric development and logging activities on known and potential eagle use areas. Four resource components include food supply, roost sites, mature riparian forest, and disturbance. In addition to actual estimates of losses in food supply (fish biomass in kg) and habitat (km/sup 2/) in one river basin, impact levels from 0 (none) to 4 (high) were assigned for each development and for each component based on themore » impacts anticipated and the estimated value of the site to eagles. Midwinter eagle surveys, aerial photography, topographic and forest stand maps, and site visits were used in the analysis. Impacts were considered additive for all but the disturbance component, which was adjusted for potential synergism between developments. Adjustments were made for mitigation before the impacts were aggregated into a single, dimensionless cumulative impact score. 50 refs., 1 fig., 1 tab.« less

The use of lead isotope analysis to identify potential sources of lead toxicosis in a juvenile bald eagle (Haliaeetus leucocephalus) with ventricular foreign bodies

USGS Publications Warehouse

Franzen-Klein, Dana; McRuer, David; Slabe, Vincent; Katzner, Todd

2018-01-01

A male juvenile bald eagle (Haliaeetus leucocephalus) was admitted to the Wildlife Center of Virginia with a left humeral fracture a large quantity of anthropogenic debris in the ventriculus, a blood lead level of 0.616 ppm, and clinical signs consistent with chronic lead toxicosis. Because of the poor prognosis for recovery and release, the eagle was euthanatized. Lead isotope analysis was performed to identify potential anthropogenic sources of lead in this bird. The lead isotope ratios in the eagle's femur (0.8773), liver (0.8761), and kidneys (0.8686) were most closely related to lead paint (0.8925), leaded gasoline (0.8450), and zinc smelting (0.8240). The lead isotope ratios were dissimilar to lead ammunition (0.8179) and the anthropogenic debris in the ventriculus. This case report documents foreign body ingestion in a free-ranging bald eagle and demonstrates the clinical utility of lead isotope analysis to potentially identify or exclude anthropogenic sources of lead poisoning in wildlife patients.

Eagle's syndrome veiling as pain of odontogenic origin: Report of two cases with cone beam computed tomography illustration

PubMed Central

Ranjan, Vikash; Rai, Shalu; Misra, Deepankar; Panjwani, Sapna

2015-01-01

Eagle's syndrome, also known as an elongated styloid process, is a condition that may be the source of craniofacial and cervical pain. It is infrequently reported but is probably more common than generally considered. The symptoms related to Eagle's syndrome can be confused with those attributed to a wide variety of facial neuralgia and or oral, dental, and temporomandibular joint diseases. In this paper, there are two cases, which reported to the Department of Oral Medicine, Diagnosis, and Maxillofacial Radiology, with a chief complaint of radiating pain in the preauricular region of the face. After radiographic investigation, these cases are considered as a case of Eagle's syndrome because of increase in the size of the styloid process. PMID:27390501

EAGLEView: A surface and grid generation program and its data management

NASA Technical Reports Server (NTRS)

Remotigue, M. G.; Hart, E. T.; Stokes, M. L.

1992-01-01

An old and proven grid generation code, the EAGLE grid generation package, is given an added dimension of a graphical interface and a real time data base manager. The Numerical Aerodynamic Simulation (NAS) Panel Library is used for the graphical user interface. Through the panels, EAGLEView constructs the EAGLE script command and sends it to EAGLE to be processed. After the object is created, the script is saved in a mini-buffer which can be edited and/or saved and reinterpreted. The graphical objects are set-up in a linked-list and can be selected or queried by pointing and clicking the mouse. The added graphical enhancement to the EAGLE system emphasizes the unique capability to construct field points around complex geometry and visualize the construction every step of the way.

Effect of prolonged incubation with copper on endothelium-dependent relaxation in rat isolated aorta

PubMed Central

Chiarugi, Alberto; Pitari, Giovanni Mario; Costa, Rosa; Ferrante, Margherita; Villari, Loredana; Amico-Roxas, Matilde; Godfraind, Théophile; Bianchi, Alfredo; Salomone, Salvatore

2002-01-01

We investigated the effects of prolonged exposure to copper (Cu2+) on vascular functioning of isolated rat aorta. Aortic rings were exposed to CuSO4 (3–24 h) in Dulbecco's modified Eagle medium with or without 10% foetal bovine serum (FBS) and then challenged with vasoconstrictors or vasodilators in the absence of Cu2+. Exposure to 2 μM Cu2+ in the absence of FBS did not modify the response to phenylephrine (PE) or acetylcholine (ACh) in aortic rings incubated for 24 h. Identical exposure in the presence of FBS increased the contractile response to 1 μM PE by 30% (P<0.05) and impaired the relaxant response to 3 μM ACh or 1 μM A23187 (ACh, from 65.7±7.1 to 6.2±1.1%, n=8; A23187, from 74.6±8.2 to 12.0±0.8%, n=6; P<0.01 for both). Cu2+ exposure did not affect the relaxant response to NO-donors. Impairment of vasorelaxation appeared 3 h after incubation with 2 μM Cu2+ and required 12 h to attain a steady state. Vasorelaxation to ACh was partially restored by 1 mM tiron (intracellular scavenger of superoxide ions; maximum relaxation 34.2±6.4%, n=10, P<0.01 vs Cu2+ alone), whereas catalase, superoxide dismutase or cycloheximide were ineffective. Twenty-four hour-exposure to 2 μM Cu2+ did not affect endothelium integrity or eNOS expression, and increased the Cu content in arterial rings from 6.8±1.1 to 18.9±2.9 ng mg−1 wet weight, n=8; P<0.01. Our results show that, in the presence of FBS, prolonged exposure to submicromolar concentrations of Cu2+ impaired endothelium-dependent vasorelaxation in aortic rings, probably through an intracellular generation of superoxide ions. PMID:12163352

Establishment and characterization of a normal melanocyte cell line derived from pig skin.

PubMed

Julé, Sophia; Bossé, Philippe; Egidy, Giorgia; Panthier, Jean-Jacques

2003-08-01

Several minipig strains develop spontaneous malignant melanoma. As a first step toward the analysis of genes involved in the tumoral progression of melanoma in these animal models, we developed culture conditions for pig melanocytes whereby melanocytes from normal epidermis can be isolated directly onto mitotically inactivated keratinocytes in Eagle's minimal essential medium supplemented with fetal calf serum, tetradecanoyl phorbol acetate (TPA) and cholera toxin. We also derived an immortal line of pigmented melanocytes from the epidermis of a healthy Meishan pig. This cell line, designated PigMel, retains differentiation function in culture, dependence on TPA and cholera toxin and a diploid chromosome number. PigMel melanocytes exhibit morphological and molecular characteristics common to normal mammalian skin melanocytes.

Suspected lead toxicosis in a bald eagle

USGS Publications Warehouse

Jacobson, E.; Carpenter, J.W.; Novilla, M.

1977-01-01

An immature bald eagle (Haliaeetus leucocephalus) was submitted to the University of Maryland, College Park, for clinical examination. The bird was thin, had green watery feces, and was unable to maintain itself in upright posture. Following radiography, the bird went into respiratory distress and died. Numerous lead shot were recovered from the gizzard, and chemical analysis of liver and kidney tissue revealed 22.9 and 11.3 ppm lead, respectively. The clinical signs, necropsy findings, and chemical analysis of the eagle were compatible with lead toxicosis.

Rover Landing Hardware at Eagle Crater, Mars

NASA Image and Video Library

2017-04-21

The bright landing platform left behind by NASA's Mars Exploration Rover Opportunity in 2004 is visible inside Eagle Crater, at upper right in this April 8, 2017, observation by NASA's Mars Reconnaissance Orbiter. Mars Reconnaissance Orbiter arrived at Mars in March 2006, more than two years after Opportunity's landing on Jan. 25, 2004, Universal Time (Jan. 24, PDT). This is the first image of Eagle Crater from the orbiter's High Resolution Imaging Science Experiment (HiRISE) camera, which has optics that include the most powerful telescope ever sent to Mars. Eagle Crater is about 72 feet (22 meters) in diameter, at 1.95 degrees south latitude, 354.47 degrees east longitude, in the Meridiani Planum region of Mars. The airbag-cushioned lander, with Opportunity folded-up inside, first hit Martian ground near the crater, then bounced and rolled right into the crater. The lander structure was four triangles, folded into a tetrahedron until after the airbags deflated. The triangular petals then opened, exposing the rover. A week later, the rover drove off (see PIA05214), and the landing platform's job was done. The spacecraft's backshell and parachute, jettisoned during final descent, are visible near the lower left corner of this scene. The blue tint of the backshell is an effect of exaggerated color, because HiRISE combines color information from red, blue-green and infrared portions of the spectrum, rather than three different visible-light colors, so its color images are not true color. Opportunity examined Eagle Crater for more than half of the rover's originally planned three-month mission, before driving east and south to larger craters. At Eagle, it found headline-making evidence that water once flowed over the surface and soaked the subsurface of the area. By the time this orbital image of the landing site was taken, about 13 years after the rover departed Eagle, Opportunity had driven more than 27 miles (44 kilometers) and was actively exploring the rim of Endeavour Crater, which is about 1,000 times as wide as Eagle. https://photojournal.jpl.nasa.gov/catalog/PIA21494

Primary culture of human thyrocytes in Transwell bicameral chamber: thyrotropin promotes polarization and epithelial barrier function.

PubMed

Nilsson, M; Husmark, J; Nilsson, B; Tisell, L E; Ericson, L E

1996-10-01

Epithelial properties of thyrocytes are difficult to maintain in conventional cell culture systems. We used bicameral chambers (Transwell) in attempts to establish a functional epithelium of thyrocytes of human origin. Thyroid follicle segments were isolated by collagenase digestion of paradenomatous tissue obtained at surgery for follicular adenoma and of tissue from glands with Graves' disease. After careful separation from connective tissue and single cells by centrifugation, the follicles were plated at high density on the collagen-coated filter of the chambers and cultured in Eagle's essential medium (EMEM) containing 10% fetal calf serum (FCS) or Coon's modified Hams medium enriched with five or six factors (5H, 6H); the latter media contained 5% FCS without (5H) or with (6H) thyrotropin (TSH). The follicles were converted into a confluent cell layer, which had similar DNA content irrespective of type of medium, after 4-6 days. Cells grown in EMEM or 5H established a transepithelial electrical resistance (R) of 200-500 omega.cm2 and was impermeable to [3H]inulin, indicating the formation of epithelial junctions. Addition of 6H to confluent cells initially cultured in EMEM or 5H caused a further increase of R, maximally to 1500 omega.cm2, along with a rise of the transepithelial potential difference; 6H promoted the monolayer formation of cells, increased the number of apical microvilli and reinforced the junctional distribution of actin, cadherin and ZO-1; 6H also enhanced the polarized secretion of [3H]leucine-labeled thyroglobulin into the apical medium. Cells from Graves' thyroid tissue established an epithelium on the filter with similar characteristics to that of normal thyrocytes; some platings contained in addition large numbers of HLA-DR positive cells with a dendritic shape. HLA-DR expression was generally absent in EMEM-or 5H-grown thyrocytes, but appeared in limited areas of the cell layer after 6H and was expressed by all epithelial cells after interferon-gamma stimulation for 48 h. We conclude that human thyrocytes form a tight and polarized epithelium when cultured on permeable filters. The polarized structure and function of the cells are positively regulated by TSH. The culture system may be useful in studies addressing the role of the epithelial phenotype (cell polarity and tight barrier) in normal thyroid function as well as in pathological processes in the thyroid, such as autoimmunity, cell transformation and tumor progression.

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2012 CFR

2012-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2013 CFR

2013-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2014 CFR

2014-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2011 CFR

2011-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

Publications - GMC 131 | Alaska Division of Geological & Geophysical

Science.gov Websites

and core from the Chevron USA Inc. Eagle Creek #1 well Authors: Unknown Publication Date: 1989 Inc. Eagle Creek #1 well: Alaska Division of Geological & Geophysical Surveys Geologic Materials

KSC-2009-2136

NASA Image and Video Library

2009-03-16

CAPE CANAVERAL, Fla. – A mature eagle stands watch near its nest, out of sight, which holds a young eagle. The nest is in a pine tree on S.R. 3 that runs through NASA's Kennedy Space Center in Florida. There are a dozen eagle nests within Kennedy and in the surrounding Merritt Island National Wildlife Refuge. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Its natural domain is from Alaska to Baja, California, and from Maine to Florida. The Merritt Island refuge also includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered. Photo credit: NASA/Ben Smegelsky

Influence of boat noises on escape behaviour of white-spotted eagle ray Aetobatus ocellatus at Moorea Island (French Polynesia).

PubMed

Berthe, Cecile; Lecchini, David

2016-02-01

The present study tested different sounds that could disturb eagle rays (Aetobatus ocellatus) during their foraging activities at Moorea, French Polynesia. Results showed that artificial white sound and single-frequency tones (40 Hz, 600 Hz or 1 kHz) did not have an effect on rays (at least 90% of rays continued to forage over sand), while playbacks of boat motor sound significantly disturbed rays during foraging activity (60% exhibited an escape behaviour). Overall, our study highlighted the negative effect of boat noises on the foraging activity of eagle rays. These noises produced by boat traffic could, however, have some positive effects for marine aquaculture if they could be used as a deterrent to repel the eagle rays, main predators of the pearl oysters. Copyright © 2016. Published by Elsevier SAS.

KSC-2009-3153

NASA Image and Video Library

2009-04-28

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, a bald eagle flies across the road in front of vehicles on the road. There are a dozen eagle nests within Kennedy and in the surrounding Merritt Island National Wildlife Refuge, some of them close to roads through the center. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Eagles' natural domain is from Alaska to Baja, California, and from Maine to Florida. The Merritt Island refuge also includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered. Photo credit: NASA/Ben Smegelsky

KSC-2009-2134

NASA Image and Video Library

2009-03-16

CAPE CANAVERAL, Fla. – On a road through NASA's Kennedy Space Center in Florida, an eagle's nest in a pine tree holds a young eagle while its parent watches on a nearby branch. There are a dozen eagle nests within Kennedy and in the surrounding Merritt Island National Wildlife Refuge. Bald eagles use a specific territory for nesting (they mate for life), winter feeding or a year-round residence. Its natural domain is from Alaska to Baja, California, and from Maine to Florida. The Merritt Island refuge also includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered. Photo credit: NASA/Ben Smegelsky

Trigeminal neuralgia post-styloidectomy in Eagle syndrome: a case report

PubMed Central

2012-01-01

Introduction Eagle syndrome is a condition characterized by an elongated (>3cm) styloid process with associated symptoms of recurrent facial or throat pain. In this report we present a case of Eagle syndrome exhibiting the typical findings of glossopharyngeal nerve involvement, as well as unusual involvement of the trigeminal nerve. Notably, this patient developed a classical trigeminal neuralgia post-styloidectomy. Case presentation A 68-year-old Caucasian woman presented with a 25-year history of dull pain along the right side of her throat, lateral neck, and jaw. Her symptoms were poorly controlled with medication until 15 years ago when she was diagnosed with Eagle syndrome, and underwent a manual fracture of her styloid process. This provided symptomatic relief until 5 years ago when the pain recurred and progressed. She underwent a styloidectomy via a lateral neck approach, which resolved the pain once again. However, 6 months ago a new onset of triggerable, electric shock-like facial pain began within the right V1 and V2 distributions. Conclusions Eagle syndrome is distressing to patients and often difficult to diagnose due to its wide variability in symptoms. It is easily confused with dental pain or temporomandibular joint disorder, leading to missed diagnoses and unnecessary procedures. Pain along the jaw and temple is an unusual but possible consequence of Eagle syndrome. An elongated styloid process should be considered a possible etiology of dull facial pain in the trigeminal distributions, in particular V3. PMID:23031688

Anesthesia with Isoflurane and Sevoflurane in the Crested Serpent Eagle (Spilornis cheela hoya): Minimum Anesthetic Concentration, Physiological Effects, Hematocrit, Plasma Chemistry and Behavioral Effects

PubMed Central

CHAN, Fang-Tse; CHANG, Geng-Ruei; WANG, Hsien-Chi; HSU, Tien-Huan

2013-01-01

ABSTRACT The initial goal of this study was to determine the minimum anesthetic concentration (MAC) for isoflurane (ISO) and sevoflurane (SEVO) for the crested serpent eagle. Next, we compared the anesthetic effects of each on the physiological effects, hematocrit, plasma chemistry values and behavior in spontaneously breathing captive adult crested serpent eagles. Sixteen eagles were randomly allocated to two groups for anesthesia with ISO (n=8) or SEVO (n=8). First, we measured the MAC values of ISO and SEVO, and four weeks later, we investigated the effect of each on the physiological effects, hematocrit (HCT) and plasma chemistry values. The MAC values of ISO and SEVO for crested serpent eagles were 1.46 ± 0.30 and 2.03 ± 0.32%, respectively. The results revealed no significant differences between the two anesthetics in induction time, while time of extubation to recovery was significantly shorter with SEVO. A time-related increase in end-tidal CO2 and decreases in body temperature and respiratory rates were observed during anesthesia with each anesthetic. There were no significant differences between the effect of the two anesthetics on heart rate, hematocrit, plasma chemistry values or respiration, although each caused minor respiration depression. We concluded that SEVO is a more effective inhalant agent than ISO for use in eagles, showing the most rapidest induction and recovery from anesthesia. PMID:23955396

Home in the heat: Dramatic seasonal variation in home range of desert golden eagles informs management for renewable energy development

USGS Publications Warehouse

Braham, Melissa A.; Miller, Tricia A.; Duerr, Adam E.; Lanzone, Michael J.; Fesnock, Amy; LaPre, Larry; Driscoll, Daniel; Katzner, Todd E.

2015-01-01

Renewable energy is expanding quickly with sometimes dramatic impacts to species and ecosystems. To understand the degree to which sensitive species may be impacted by renewable energy projects, it is informative to know how much space individuals use and how that space may overlap with planned development. We used global positioning system–global system for mobile communications (GPS-GSM) telemetry to measure year-round movements of golden eagles (Aquila chrysaetos) from the Mojave Desert of California, USA. We estimated monthly space use with adaptive local convex hulls to identify the temporal and spatial scales at which eagles may encounter renewable energy projects in the Desert Renewable Energy Conservation Plan area. Mean size of home ranges was lowest and least variable from November through January and greatest in February–March and May–August. These monthly home range patterns coincided with seasonal variation in breeding ecology, habitat associations, and temperature. The expanded home ranges in hot summer months included movements to cooler, prey-dense, mountainous areas characterized by forest, grasslands, and scrublands. Breeding-season home ranges (October–May) included more lowland semi-desert and rock vegetation. Overlap of eagle home ranges and focus areas for renewable energy development was greatest when eagle home ranges were smallest, during the breeding season. Golden eagles in the Mojave Desert used more space and a wider range of habitat types than expected and renewable energy projects could affect a larger section of the regional population than was previously thought.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Powell, D.R.; Hutchinson, J.L.

Eagle 11 is a prototype analytic model derived from the integration of the low resolution Eagle model with the high resolution SIMNET model. This integration promises a new capability to allow for a more effective examination of proposed or existing combat systems that could not be easily evaluated using either Eagle or SIMNET alone. In essence, Eagle II becomes a multi-resolution combat model in which simulated combat units can exhibit both high and low fidelity behavior at different times during model execution. This capability allows a unit to behave in a highly manner only when required, thereby reducing the overallmore » computational and manpower requirements for a given study. In this framework, the SIMNET portion enables a highly credible assessment of the performance of individual combat systems under consideration, encompassing both engineering performance and crew capabilities. However, when the assessment being conducted goes beyond system performance and extends to questions of force structure balance and sustainment, then SISMNET results can be used to ``calibrate`` the Eagle attrition process appropriate to the study at hand. Advancing technologies, changes in the world-wide threat, requirements for flexible response, declining defense budgets, and down-sizing of military forces motivate the development of manpower-efficient, low-cost, responsive tools for combat development studies. Eagle and SIMNET both serve as credible and useful tools. The integration of these two models promises enhanced capabilities to examine the broader, deeper, more complex battlefield of the future with higher fidelity, greater responsiveness and low overall cost.« less

Questa baseline and premining ground-water quality investigation. 8. Lake-sediment geochemical record from 1960 to 2002, Eagle Rock and Fawn Lakes, Taos County, New Mexico

USGS Publications Warehouse

Church, S.E.; Fey, D.L.; Marot, M.E.

2005-01-01

Geochemical studies of lake sediment from Eagle Rock Lake and upper Fawn Lake were conducted to evaluate the effect of mining at the Molycorp Questa porphyry molybdenum deposit located immediately north of the Red River. Two cores were taken, one from each lake near the outlet where the sediment was thinnest, and they were sampled at 1-cm intervals to provide geochemical data at less than 1-year resolution. Samples from the core intervals were digested and analyzed for 34 elements using ICP-AES (inductively coupled plasma-atomic emission spectrometry). The activity of 137Cs has been used to establish the beginning of sedimentation in the two lakes. Correlation of the geochemistry of heavy-mineral suites in the cores from both Fawn and Eagle Rock Lakes has been used to develop a sedimentation model to date the intervals sampled. The core from upper Fawn Lake, located upstream of the deposit, provided an annual sedimentary record of the geochemical baseline for material being transported in the Red River, whereas the core from Eagle Rock Lake, located downstream of the deposit, provided an annual record of the effect of mining at the Questa mine on the sediment in the Red River. Abrupt changes in the concentrations of many lithophile and deposit-related metals occur in the middle of the Eagle Rock Lake core, which we correlate with the major flood-of-record recorded at the Questa gage at Eagle Rock Lake in 1979. Sediment from the Red River collected at low flow in 2002 is a poor match for the geochemical data from the sediment core in Eagle Rock Lake. The change in sediment geochemistry in Eagle Rock Lake in the post-1979 interval is dramatic and requires that a new source of sediment be identified that has substantially different geochemistry from that in the pre-1979 core interval. Loss of mill tailings from pipeline breaks are most likely responsible for some of the spikes in trace-element concentrations in the Eagle Rock Lake core. Enrichment of Al2O3, Cu, and Zn occurred as a result of chemical precipitation of these metals from ground water upstream in the Red River. Comparisons of the geochemistry of the post-1979 sediment core with both mine wastes and with premining sediment from the vicinity of the Questa mine indicate that both are possible sources for this new component of sediment. Existing data have not resolved this enigma.

A bald eagle at KSC

NASA Technical Reports Server (NTRS)

2000-01-01

High in a pine tree on the grounds of the Kennedy Space Center, a bald eagle perches on a branch. The Southern Bald Eagle ranges throughout Florida and along the coasts of California, Texas, Louisiana, and the south Atlantic states. Bald eagles are listed as endangered in the U.S., except in five states where they are listed as threatened. The number of nesting pairs of the southern race once numbered several thousand; recent estimates are only 350-375. Most of the southern race nests in Florida. Eagles arrive at KSC during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. KSC shares a boundary with the Merritt Island National Wildlife Refuge, which encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

KSC-2011-7693

NASA Image and Video Library

2011-10-24

CAPE CANAVERAL, Fla. – On State Road 3 near the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, two bald eagles scout the terrain from their vantage point atop a pole. There are 18 active eagle nests within Kennedy's boundaries, including several in the vicinity of the landing strip. Bald eagles mate for life, choosing the tops of large trees to build nests, which they typically use and enlarge each year. Nests may reach 10 feet across and weigh half a ton. The birds travel great distances, but usually return to breeding grounds within 100 miles of the place where they were raised. Bald eagles may live 15 to 25 years in the wild. The Merritt Island National Wildlife Refuge coexists with Kennedy Space Center and provides a habitat for 330 species of birds including the bald eagle. A variety of other wildlife - 117 kinds of fish, 65 types of amphibians and reptiles, 31 different mammals, and 1,045 species of plants - also inhabit the refuge. For information on the refuge, visit http://www.fws.gov/merrittisland/Index.html. For information on Kennedy Space Center, visit http://www.nasa.gov/kennedy. Photo credit: NASA/Ken Thornsley

KSC-2011-7694

NASA Image and Video Library

2011-10-24

CAPE CANAVERAL, Fla. – On State Road 3 near the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, two bald eagles scout the terrain from their vantage point atop a pole. There are 18 active eagle nests within Kennedy's boundaries, including several in the vicinity of the landing strip. Bald eagles mate for life, choosing the tops of large trees to build nests, which they typically use and enlarge each year. Nests may reach 10 feet across and weigh half a ton. The birds travel great distances, but usually return to breeding grounds within 100 miles of the place where they were raised. Bald eagles may live 15 to 25 years in the wild. The Merritt Island National Wildlife Refuge coexists with Kennedy Space Center and provides a habitat for 330 species of birds including the bald eagle. A variety of other wildlife - 117 kinds of fish, 65 types of amphibians and reptiles, 31 different mammals, and 1,045 species of plants - also inhabit the refuge. For information on the refuge, visit http://www.fws.gov/merrittisland/Index.html. For information on Kennedy Space Center, visit http://www.nasa.gov/kennedy. Photo credit: NASA/Ken Thornsley

To migrate, stay put, or wander? Varied movement strategies in bald eagles (Haliaeetus leucocephalus).

PubMed

Wheat, Rachel E; Lewis, Stephen B; Wang, Yiwei; Levi, Taal; Wilmers, Christopher C

2017-01-01

Quantifying individual variability in movement behavior is critical to understanding population-level patterns in animals. Here, we explore intraspecific variation in movement strategies of bald eagles ( Haliaeetus leucocephalus ) in the north Pacific, where there is high spatiotemporal resource variability. We tracked 28 bald eagles (five immature, 23 adult) using GPS transmitters between May 2010 and January 2016. We found evidence of four movement strategies among bald eagles in southeastern Alaska and western Canada: breeding individuals that were largely sedentary and remained near nest sites year-round, non-breeding migratory individuals that made regular seasonal travel between northern summer and southern winter ranges, non-breeding localized individuals that displayed fidelity to foraging sites, and non-breeding nomadic individuals with irregular movement. On average, males traveled farther per day than females. Most nomadic individuals were immature, and all residential individuals (i.e. breeders and localized birds) were adults. Alternative movement strategies among north Pacific eagles are likely associated with the age and sex class, as well as breeding status, of an individual. Intraspecific variation in movement strategies within the population results in different space use patterns among contingents, which has important implications for conservation and management.

Bald eagles view their territory atop a utility pole at KSC

NASA Technical Reports Server (NTRS)

2000-01-01

This pair of bald eagles appear unhappy as the focus of the camera while they perch together on the top of a utility pole at Kennedy Space Center. The Center counts more than half a dozen bald eagles among the denizens of its site, especially since KSC shares a boundary with the Merritt Island National Wildlife Refuge. The Southern bald eagle is no stranger to Florida as it ranges throughout the state and along the coasts of California, Texas, Louisiana and south Atlantic states. Most southern Florida eagles nesting at KSC arrive during late summer and leave for the north in late spring. They move to nest sites in October and November and lay one to three eggs. The young fledge from February to April. The Refuge encompasses 92,000 acres that are a habitat for more than 331 species of birds, 31 mammals, 117 fishes, and 65 amphibians and reptiles. The marshes and open water of the refuge provide wintering areas for 23 species of migratory waterfowl, as well as a year-round home for great blue herons, great egrets, wood storks, cormorants, brown pelicans and other species of marsh and shore birds, as well as a variety of insects.

Detail view of bracket, arched window and eagle from building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view of bracket, arched window and eagle from building 18 section. Jet Lowe, Haer staff photographer, summer 1995 - Naval Base Philadelphia-Philadelphia Naval Shipyard, Machine Shops, League Island, Philadelphia, Philadelphia County, PA

50 CFR 22.27 - Removal of eagle nests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... when the Service determines it is necessary to prevent eagles from re-nesting in the vicinity. (7) You... Migratory Bird Permit Office (http://www.fws.gov/permits/mbpermits/addresses.html) at the earliest possible...

50 CFR 22.27 - Removal of eagle nests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... when the Service determines it is necessary to prevent eagles from re-nesting in the vicinity. (7) You... Migratory Bird Permit Office (http://www.fws.gov/permits/mbpermits/addresses.html) at the earliest possible...

50 CFR 22.27 - Removal of eagle nests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... when the Service determines it is necessary to prevent eagles from re-nesting in the vicinity. (7) You... Migratory Bird Permit Office (http://www.fws.gov/permits/mbpermits/addresses.html) at the earliest possible...

50 CFR 22.27 - Removal of eagle nests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... when the Service determines it is necessary to prevent eagles from re-nesting in the vicinity. (7) You... Migratory Bird Permit Office (http://www.fws.gov/permits/mbpermits/addresses.html) at the earliest possible...

The biologic role of ganglioside in neuronal differentiation--effects of GM1 ganglioside on human neuroblastoma SH-SY5Y cells.

PubMed Central

Lee, M. C.; Lee, W. S.; Park, C. S.; Juhng, S. W.

1994-01-01

Human neuroblastoma SH-SY5Y cell is a cloned cell line which has many attractive features for the study of neuronal proliferation and neurite outgrowth, because it has receptors for insulin, IGF-I and PDGF. Gangliosides are sialic acid containing glycosphingolipids which form an integral part of the plasma membrane of many mammalian cells. They inhibit cell growth mediated by tyrosine kinase receptors and ligand-stimulated tyrosine kinase activity, and autophosphorylation of EGF(epidermal growth factor) and PDGF receptors. The experiment was designed to study the effects of GM1 ganglioside on growth of human neuroblastoma SH-SY5Y cells stimulated with trophic factor in vitro. The cells were plated in Eagle's minimum essential medium without serum. The number and morphologic change of SH-SY5Y cells were evaluated in the serum free medium added GM1 ganglioside with insulin or PDGF. SH-SY5Y cells were maintained for six days in serum-free medium, and then cultured for over two weeks in serum-free medium containing either insulin or PDGF. The effect of insulin on cell proliferation developed earlier and was more potent than that of PDGF. These proliferative effects were inhibited by GM1 ganglioside, and the cells showed prominent neurites outgrowth. These findings suggest that GM1 ganglioside inhibits the cell proliferation mediated by tyrosine kinase receptors and directly induces neuritogenesis as one of the neurotrophic factors. PMID:7986393

DDE poisoning in an adult bald eagle

USGS Publications Warehouse

Garcelon, D.K.; Thomas, N.J.

1997-01-01

A 12-year-old female bald eagle (Haliaeetus leucocephalus) was found in May 1993 on Santa Catalina Island, California (USA), in a debilitated condition, exhibiting ataxia and tremors; it died within hours. On necropsy, the bird was emaciated but had no evidence of disease or physical injury. Chemical analyses were negative for organophosphorus pesticides and lead poisoning. High concentrations of DDE (wet weight basis) were found in the brain (212 ppm), liver (838 ppm), and serum (53 ppm). Mobilization of DDE, from depleted fat deposits, probably resulted in the lethal concentration in the eagle's brain.

Wildlife - Bald Eagle

NASA Image and Video Library

2007-03-20

High in a pine tree at NASA's Kennedy Space Center, an adult bald eagle (right) and a fledgling keep watch from their nest. There are approximately a dozen active bald eagle nests both in KSC and in the Merritt Island National Wildlife Refuge, which surrounds KSC. The refuge includes several wading bird rookeries, many osprey nests, up to 400 manatees during the spring, and approximately 2,500 Florida scrub jays. It also is a major wintering area for migratory birds. More than 500 species of wildlife inhabit the refuge, with 15 considered federally threatened or endangered.

Whooping crane preyed upon by golden eagle

USGS Publications Warehouse

Windingstad, Ronald M.; Stiles, Harry E.; Drewien, Roderick C.

1981-01-01

The Golden Eagle (Aquila chrysaetos) is the largest predatory bird in North America and is well known for its predatory abilities. Attacks have been reported on mammals such as whitetail jackrabbits (Lepus townsendi) (McGahan 1967, J. Wildl. Mgmt. 31: 496), pronghorn antelope (Antilocapra americana) (Bruhns 1970, Can. Field-Natur. 84: 301), Mallards (Anas platyrhynchos) (Kelleher and O'Malia 1971, Auk 88: 186), and Great Blue Herons (Ardea herodias) (Carnie 1954, Condor 56: 3). This communication describes an attack on an immature Whooping Crane (Grus americana) by a Golden Eagle and the subsequent necropsy findings.

2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in extracts of Baltic white-tailed sea eagles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koistinen, J.; Giesy, J.P.; Koivusaari, J.

Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin-(TCDD) equivalents were measured in extracts of Baltic white-tailed sea eagle tissues. Extracts of salmon, ringed seal, and grey seal were analyzed as other predatory species of the same area. Concentrations in eagle and seal tissues were greater than those in salmon. Concentrations of TCDD equivalents (TCDD-EQs) determined by the H4IIE bioassay were compared with toxic equivalents (TEQs) derived from instrumental chemical analyses in fractions containing polychlorinated dibenzo-P-dioxins and polychlorinated dibenzofurans (PCDD/PCDF) or coplanar polychlorinated biphenyls (PCBs). Toxic equivalents were calculated by use of an additive model in which the product of the concentrations of instrumentally measured individualmore » congeners were multiplied by their TCDD equivalency factors and were summed to give a total concentration of TEQs. The TCDD-EQs were compared with TEQs to develop a mass balance to determine whether all the TCDD-like activity was accounted for. The TEQs determined by chemical analyses for coplanar PCBs was 770 pg/g fw, and that of PCDD/PCDFs was 270 pg/g fw in this eagle. Thus, concentrations of TCDD-EQs were approx. 20% greater than those of TEQs. The true difference in activities is probably greater because of lower recoveries and infra-additivities among congeners in the bioassay. This indicates that there are compounds present in the extracts that can contribute to the total concentrations of TCDD-EQs in white-tailed sea eagle eggs to the no-observable-adverse-effect concentration, ranged from 7.3 to 141. This indicates that current concentrations of TCDD-EQs in these eggs are likely causing adverse effects in the Baltic populations of white-tailed sea eagles. This study indicated that the H4IIE bioassay is useful for monitoring the presence and biological activity of TCDD-like compounds in environmental samples like white-tailed sea eagles.« less

Relationships between bald eagle productivity and dynamics of fish populations and fisheries in the Wisconsin waters of Lake Superior, 1983-1999

USGS Publications Warehouse

Hoff, Michael H.; Meyer, Michael W.; Van Stappen, Julie; Fratt, Thomas W.

2004-01-01

Bald eagle (Haliaeetus leucocephalus) abundance declined in the 1950s and 1960s along the Wisconsin waters of Lake Superior, and were nearly absent along Wisconsin's Lake Superior shoreline. The population began to increase again between 1980 and 1983, and since then bald eagles nesting on islands along Wisconsin's Lake Superior shoreline (i.e., Apostle Islands) reproduced at a lower rate than have those nesting along the mainland shoreline of the lake and inland. Recent research indicated that bioaccumulation of toxic chemicals in the aquatic food chain no longer limits bald eagle reproduction there, and that productivity at island nests was lower than at mainland nests and inland nests as the result of low food availability. Management agencies have sought models that accurately predict productivity and explain ecological relationships, but no satisfactory models had previously been developed. Modeling was conducted here to determine which factors best explained productivity variability. The Ricker stock-recruitment model derived from only the bivariate breeding pair and productivity data explained only 1% of the variability in productivity data. The functional relationship that explained the greatest amount of productivity variation (83%) included the number of breeding pairs, burbot (Lota lota) biomass, longnose sucker (Catostomus catostomus) biomass, and commercial harvest of nontarget fishes. Model results were interpreted to mean that productivity was positively affected by populations of burbot and longnose sucker, which are important prey items, and by commercial fishermen feeding nontarget fish to bald eagles. Harvest of nontarget fishes by tribal fishermen and burbot and longnose sucker populations have not tended to change during the entire study period, although the burbot population has declined since 1991. Therefore, bald eagle productivity is not predicted to increase unless burbot, longnose sucker, or other preferred prey of bald eagles increases in the Apostle Islands.

Care, food consumption, and behavior of bald eagles used in DDT tests

USGS Publications Warehouse

Chura, N.J.; Stewart, P.A.

1967-01-01

Twenty-seven Bald Eagles captured in southeastern Alaska were used in feeding tests to determine the effects of DDT in the diet.....Trapping and housing of eagles are discussed. Various aspects of eagle behavior and handling techniques are also presented. Recommendations are made for preventing injuries and increasing the comfort of captive birds.....The 1962 test birds consumed an average of 274 grams per bird day with a range of 109 to 401 grams per day between birds. Average food intake was 254 grams per bird day for the 1963 test birds with a range of 194 to 324 grams per day between birds.....Weight losses varied from 23 to 49 per cent of normal body weight for the 7 birds which died in the 1962 tests. Tremors and death occurred first for birds on the highest dosage and progressively later for birds on the lower dosages.

Program Monitoring with LTL in EAGLE

NASA Technical Reports Server (NTRS)

Barringer, Howard; Goldberg, Allen; Havelund, Klaus; Sen, Koushik

2004-01-01

We briefly present a rule-based framework called EAGLE, shown to be capable of defining and implementing finite trace monitoring logics, including future and past time temporal logic, extended regular expressions, real-time and metric temporal logics (MTL), interval logics, forms of quantified temporal logics, and so on. In this paper we focus on a linear temporal logic (LTL) specialization of EAGLE. For an initial formula of size m, we establish upper bounds of O(m(sup 2)2(sup m)log m) and O(m(sup 4)2(sup 2m)log(sup 2) m) for the space and time complexity, respectively, of single step evaluation over an input trace. This bound is close to the lower bound O(2(sup square root m) for future-time LTL presented. EAGLE has been successfully used, in both LTL and metric LTL forms, to test a real-time controller of an experimental NASA planetary rover.

Sublethal Lead Exposure Alters Movement Behavior in Free-Ranging Golden Eagles.

PubMed

Ecke, Frauke; Singh, Navinder J; Arnemo, Jon M; Bignert, Anders; Helander, Björn; Berglund, Åsa M M; Borg, Hans; Bröjer, Caroline; Holm, Karin; Lanzone, Michael; Miller, Tricia; Nordström, Åke; Räikkönen, Jannikke; Rodushkin, Ilia; Ågren, Erik; Hörnfeldt, Birger

2017-05-16

Lead poisoning of animals due to ingestion of fragments from lead-based ammunition in carcasses and offal of shot wildlife is acknowledged globally and raises great concerns about potential behavioral effects leading to increased mortality risks. Lead levels in blood were correlated with progress of the moose hunting season. Based on analyses of tracking data, we found that even sublethal lead concentrations in blood (25 ppb, wet weight), can likely negatively affect movement behavior (flight height and movement rate) of free-ranging scavenging Golden Eagles (Aquila chrysaetos). Lead levels in liver of recovered post-mortem analyzed eagles suggested that sublethal exposure increases the risk of mortality in eagles. Such adverse effects on animals are probably common worldwide and across species, where game hunting with lead-based ammunition is widespread. Our study highlights lead exposure as a considerably more serious threat to wildlife conservation than previously realized and suggests implementation of bans of lead ammunition for hunting.

The massive star binary fraction in young open clusters - II. NGC6611 (Eagle Nebula)

NASA Astrophysics Data System (ADS)

Sana, H.; Gosset, E.; Evans, C. J.

2009-12-01

Based on a set of over 100 medium- to high-resolution optical spectra collected from 2003 to 2009, we investigate the properties of the O-type star population in NGC6611 in the core of the Eagle Nebula (M16). Using a much more extended data set than previously available, we revise the spectral classification and multiplicity status of the nine O-type stars in our sample. We confirm two suspected binaries and derive the first SB2 orbital solutions for two systems. We further report that two other objects are displaying a composite spectrum, suggesting possible long-period binaries. Our analysis is supported by a set of Monte Carlo simulations, allowing us to estimate the detection biases of our campaign and showing that the latter do not affect our conclusions. The absolute minimal binary fraction in our sample is fmin = 0.44 but could be as high as 0.67 if all the binary candidates are confirmed. As in NGC6231 (see Paper I), up to 75 per cent of the O star population in NGC6611 are found in an O+OB system, thus implicitly excluding random pairing from a classical IMF as a process to describe the companion association in massive binaries. No statistical difference could be further identified in the binary fraction, mass-ratio and period distributions between NGC6231 and NGC 6611, despite the difference in age and environment of the two clusters.

Physiological responses of somaclonal variants of triploid bermudagrass (Cynodon transvaalensis x Cynodon dactylon) to drought stress.

PubMed

Lu, Shaoyun; Chen, Chuanhao; Wang, Zhongcheng; Guo, Zhenfei; Li, Haihang

2009-03-01

Eight somaclonal variants with enhanced drought tolerance were isolated from regenerated plants of triploid bermudagrass (Cynodon dactylon x Cynodon transvaalensis cv., TifEagle). Three of them (10-17, 89-02, 117-08) with strong drought tolerance were selected for investigations of physiological responses to drought stress. Compared to the parent control, TifEagle, the somaclonal variants had higher relative water contents and relative growth, and lower ion leakages in the greenhouse tests, while no difference in evapotranspirational water losses and soil water contents was observed between the variants and TifEagle. The variants also had less leaf firing in the field tests under drought stress. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities decreased gradually in responses to drought stress in all plants and exhibited negative correlations with ion leakage, indicating that the declined activities of these antioxidant enzymes were associated with drought injury in the triploid bermudagrass. However, CAT activities were significantly higher in all three variants than in TifEagle during drought stress. Two variants, 10-17 and 89-02, also had significantly higher APX activities than TifEagle before and during the first 4 days of drought treatments. These two lines also showed higher SOD activities after prolonged drought stress. Proline, total soluble sugars and sucrose were accumulated under drought stress in all plants and exhibited positive correlations with ion leakage. More proline and sugars were accumulated in TifEagle than in the variants. The results indicated that higher activities of the antioxidant enzymes in the variants during drought stress are associated with their increased drought tolerance.

Eagle's Syndrome

PubMed Central

Pinheiro, Thaís Gonçalves; Soares, Vítor Yamashiro Rocha; Ferreira, Denise Bastos Lage; Raymundo, Igor Teixeira; Nascimento, Luiz Augusto; Oliveira, Carlos Augusto Costa Pires de

2013-01-01

Summary Introduction: Eagle's syndrome is characterized by cervicopharyngeal signs and symptoms associated with elongation of the styloid apophysis. This elongation may occur through ossification of the stylohyoid ligament, or through growth of the apophysis due to osteogenesis triggered by a factor such as trauma. Elongation of the styloid apophysis may give rise to intense facial pain, headache, dysphagia, otalgia, buzzing sensations, and trismus. Precise diagnosis of the syndrome is difficult, and it is generally confounded by other manifestations of cervicopharyngeal pain. Objective: To describe a case of Eagle's syndrome. Case Report: A 53-year-old man reported lateral pain in his neck that had been present for 30 years. Computed tomography (CT) of the neck showed elongation and ossification of the styloid processes of the temporal bone, which was compatible with Eagle's syndrome. Surgery was performed for bilateral resection of the stylohyoid ligament by using a transoral and endoscopic access route. The patient continued to present pain laterally in the neck, predominantly on his left side. CT was performed again, which showed elongation of the styloid processes. The patient then underwent lateral cervicotomy with resection of the stylohyoid process, which partially resolved his painful condition. Final Comments: Patients with Eagle's syndrome generally have a history of chronic pain. Appropriate knowledge of this disease is necessary for adequate treatment to be provided. The importance of diagnosing this uncommon and often unsuspected disease should be emphasized, given that correct clinical-surgical treatment is frequently delayed. The diagnosis of Eagle's syndrome is clinical and radiographic, and the definitive treatment in cases of difficult-to-control pain is surgical. PMID:25992033

Golden Eagle Migratory Behaviors in Response to Arctic Warming

NASA Astrophysics Data System (ADS)

LaPoint, S.; Bohrer, G.; Davidson, S. C.; Gurarie, E.; Mahoney, P.; Boelman, N.

2017-12-01

Understanding how animals adapt to climate change is a conservation priority, particularly in arctic landscapes where these changes are accelerated. Doing so however, remains challenging because animal behavior datasets are typically conducted at site- or population-specific scales and are often short term (e.g., 2-3 years). We have overcome this challenge by compiling a long-term (25 years), large-scale (northwestern North America) dataset of > 0.5 million locations collected via 86 adult-aged golden eagles (Aquila chrysaetos) fitted with satellite and GPS data loggers. We used mechanistic range shift analyses to identify the locations and dates when each eagle performed a behavioral switch from a stationary phase (e.g., over-wintering or breeding) to migration and vice-versa. We annotated these spatio-temporal data with a suite of environmental data, including: %snow cover, time-to snow cover, time-to greening, air temperature, and wind direction and magnitude. Preliminary generalized additive mixed-models suggest these eagles have performed significant shifts in their departure dates, yet their arrival dates have remained relatively consistent. We will use a survival analysis (e.g., Cox proportional-hazard regression model) to quantify the influence of the environmental variables on these dates. It appears golden eagles migrating across northwestern North America are adapting to changes in the timing and duration of artic winters, by arriving to their northern breeding grounds earlier every spring, presumably to extend their breeding and chick rearing phases. Golden eagles exhibit some resiliency to changes in the arctic climate, but further work is warranted across other taxa and populations.

Energy Intensity and Greenhouse Gas Emissions from Oil Production in the Eagle Ford Shale

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yeh, Sonia; Ghandi, Abbas; Scanlon, Bridget R.

A rapid increase in horizontal drilling and hydraulic fracturing in shale and “tight” formations that began around 2000 has resulted in record increases in oil and natural gas production in the U.S. This study examines energy consumption and greenhouse gas (GHG) emissions from crude oil and natural gas produced from ~8,200 wells in the Eagle Ford Shale in southern Texas from 2009 to 2013. Our system boundary includes processes from primary exploration wells to the refinery entrance gate (henceforth well-to-refinery or WTR). The Eagle Ford includes four distinct production zones—black oil (BO), volatile oil (VO), condensate (C), and dry gasmore » (G) zones—with average monthly gas-to-liquids ratios (thousand cubic feet per barrel—Mcf/bbl) varying from 0.91 in the BO zone to 13.9 in the G zone. Total energy consumed in drilling, extracting, processing, and operating an Eagle Ford well is ~1.5% of the energy content of the produced crude and gas in the BO and VO zones, compared with 2.2% in the C and G zones. On average, the WTR GHG emissions of gasoline, diesel, and jet fuel derived from crude oil produced in the BO and VO zones in the Eagle Ford play are 4.3, 5.0, and 5.1 gCO2e/MJ, respectively. Comparing with other known conventional and unconventional crude production where upstream GHG emissions are in the range 5.9–30 gCO2e/MJ, oil production in the Eagle Ford has lower WTR GHG emissions.« less

Enhancing Effects of Carbon Dioxide on the Primary Isolation of Acid-Fast Bacilli in a Modified Lowenstein-Jensen Medium

PubMed Central

Gruft, Howard; Loder, Arthur

1971-01-01

Specimens inoculated on modified Lowenstein-Jensen medium were incubated aerobically and in an atmosphere of 8% CO2. Sixteen per cent of the positive specimens grew Mycobacterium tuberculosis only after incubation in CO2. PMID:5002315

Publications - GMC 148 | Alaska Division of Geological & Geophysical

Science.gov Websites

feet) from the Chevron USA Eagle Creek #1 well Authors: Weber, F.F. Publication Date: 1989 Publisher ., 1989, Visual kerogen and TAI data of select cuttings (1530 - 12000 feet) from the Chevron USA Eagle

Publications - GMC 75 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

DGGS GMC 75 Publication Details Title: Vitrinite reflectance data for the Chevron U.S.A. Inc. Eagle Chevron U.S.A. Inc. Eagle Creek #1 well: Alaska Division of Geological & Geophysical Surveys Geologic

77 FR 33765 - Proposed Information Collection; Bald Eagle Post-delisting Monitoring

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-07

... this IC, contact Hope Grey at [email protected] (email) or 703-358- 2482 (telephone). SUPPLEMENTARY... of recovered species be conducted for not less than 5 years, the life history of bald eagles is such...

17. Historic American Buildings Survey R. Randolph Langenbach, Photographer August ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Historic American Buildings Survey R. Randolph Langenbach, Photographer August 28, 1967 EAGLE MILL, BELT DRIVEN SHAFT FOR IMPARTING POWER TO THE TEXTILE MACHINERY - Crown & Eagle Mills, 123 Hartford Avenue East, North Uxbridge, Worcester County, MA

Epizootic vacuolar myelinopathy of the central nervous system of bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana)

USGS Publications Warehouse

Thomas, N.J.; Meteyer, C.U.; Sileo, L.

1998-01-01

Unprecedented mortality occurred in bald eagles (Haliaeetus leucocephalus) at DeGray Lake, Arkansas, during the winters of 1994-1995 and 1996-1997. The first eagles were found dead during November, soon after arrival from fall migration, and deaths continued into January during both episodes. In total, 29 eagles died at or near DeGray Lake in the winter of 1994-1995 and 26 died in the winter of 1996-1997; no eagle mortality was noted during the same months of the intervening winter or in the earlier history of the lake. During the mortality events, sick eagles were observed overflying perches or colliding with rock walls. Signs of incoordination and limb paresis were also observed in American coots (Fulica americana) during the episodes of eagle mortality, but mortality in coots was minimal. No consistent abnormalities were seen on gross necropsy of either species. No microscopic findings in organs other than the central nervous system (CNS) could explain the cause of death. By light microscopy, all 26 eagles examined and 62/77 (81%) coots had striking, diffuse, spongy degeneration of the white matter of the CNS. Vacuolation occurred in all myelinated CNS tissue, including the cerebellar folia and medulla oblongata, but was most prominent in the optic tectum. In the spinal cord, vacuoles were concentrated near the gray matter, and occasional swollen axons were seen. Vacuoles were uniformly present in optic nerves but were not evident in the retina or peripheral or autonomic nerves. Cellular inflammatory response to the lesion was distinctly lacking. Vacuoles were 8-50 microns in diameter and occurred individually, in clusters, or in rows. In sections stained by luxol fast blue/periodic acid-Schiff stain, the vacuoles were delimited and transected by myelin strands. Transmission electron microscopy revealed intramyelinic vacuoles formed in the myelin sheaths by splitting of one or more myelin lamellae at the intraperiodic line. This lesion is characteristic of toxicity from hexachlorophene, triethyltin, bromethalin, isonicotinic acid hydrazide, and certain exotic plant toxins; however, despite exhaustive testing, no etiology was determined for the DeGray Lake mortality events. This is the first report of vacuolar myelinopathy associated with spontaneous mortality in wild birds.

Shikonin Production by Callus Culture of Onosma bulbotrichom as Active Pharmaceutical Ingredient

PubMed Central

Bagheri, Fereshteh; Tahvilian, Reza; Karimi, Naser; Chalabi, Maryam; Azami, Mahsa

2018-01-01

The objective of this research was in-vitro germination and callus induction of Onosma bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family. For germination, the seeds were cultured on growth regulator-free MS medium and for callus induction, seeds were sown on modified MS medium containing different concentrations of kinetin (kn)- Indole-3-acetic acid (IAA) and kn- 2,4-D (2,4-dichlorophenoxyacetic acid), respectively. The plates were maintained in the dark at growth chamber. After 7 days seed germination on hormone-free medium and after 10 days callus initiation on modified medium in the presence of hormones was occurred. The maximum pigmented callus (100%) was observed on modified MS medium with a combination of 0.2 mg.L-1 IAA + 2.10 mg.L-1 kn. Shikonin determination was performed by HPLC method. In addition, total hydroxynaphtoquinons as polyphenols in sum of callus and culture medium were measured by spectrophotometric method and revealed that total naphtoquinones content at IAA was more than 2, 4-D. PMID:29881407

Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions

PubMed Central

Nydam, Seth D.; Shah, Devendra H.; Call, Douglas R.

2014-01-01

Vibrio parahaemolyticus is an emerging bacterial pathogen capable of causing inflammatory gastroenteritis, wound infections, and septicemia. As a food-borne illness, infection is most frequently associated with the consumption of raw or undercooked seafood, particularly shellfish. It is the primary cause of Vibrio-associated food-borne illness in the United States and the leading cause of food-borne illness in Japan. The larger of its two chromosomes harbors a set of genes encoding type III section system 1 (T3SS1), a virulence factor present in all V. parahaemolyticus strains that is similar to the Yersinia ysc T3SS. T3SS1 translocates effector proteins into eukaryotic cells where they induce changes to cellular physiology and modulate host-pathogen interactions. T3SS1 is also responsible for cytotoxicity toward several different cultured cell lines as well as mortality in a mouse model. Herein we used RNA-seq to obtain global transcriptome patterns of V. parahaemolyticus under conditions that either induce [growth in Dulbecco's Modified Eagle Medium (DMEM) media, in trans expression of transcriptional regulator exsA] or repress T3SS1 expression (growth in LB-S media, in trans exsD expression) and during infection of HeLa cells over time. Comparative transcriptomic analysis demonstrated notable differences in the expression patterns under inducing conditions and was also used to generate an expression profile of V. parahaemolyticus during infection of HeLa cells. In addition, we identified several new genes that are associated with T3SS1 expression and may warrant further study. PMID:24478989

Antiviral activity of Quercus persica L.: High efficacy and low toxicity

PubMed Central

Karimi, Ali; Moradi, Mohammad-Taghi; Saeedi, Mojtaba; Asgari, Sedigheh; Rafieian-kopaei, Mahmoud

2013-01-01

Background: Drug-resistant strain of Herpes simplex virus type 1 (HSV-I) has increased the interest in the use of natural substances. Aims: This study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of a traditionally used herbal plant, Quercus persica L., on HSV-1 replication on baby hamster kidney (BHK) cells. Setting: The study was conducted in Shahrekord University of Medical Sciences, Iran. Design: This was an experimental study. Materials and Methods: BHK cells were grown in monolayer culture with Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum and plated onto 48-well culture plates. Fifty percent cytotoxic concentration (CC50%) of Q. persica L. on BHK cells was determined. Subsequently, 50% inhibitory concentration (IC50%) of the extract on replication of HSV-1 both in interacellular and exteracellular cases was assessed. Statistical Analysis: Statistic Probit model was used for statistical analysis. The dose-dependent effect of antiviral activity of the extracts was determined by linear regression. Results: Q. persica L. had no cytotoxic effect on this cell line. There was significant relationship between the concentration of the extract and cell death (P<0.01). IC50s of Q. persica L. on HSV-1, before and after attachment to BHK cells were 1.02 and 0.257 μg/mL, respectively. There was significant relationship between the concentration of this extract and inhibition of cytopathic effect (CPE) (P<0.05). Antioxidant capacity of the extract was 67.5%. Conclusions: The hydroalchoholic extract of Q. persica L. is potentially an appropriate and promising anti herpetic herbal medicine. PMID:24516836

Sensitivity of cultured skin fibroblasts from patients with neurofibromatosis to DNA-damaging agents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woods, W.G.; McKenzie, B.; Letourneau, M.A.

Neurofibromatosis (NF) is an autosomal dominant disorder associated with various constitutional abnormalities as well as a striking predisposition for malignant and nonmalignant neoplasms, both in cells originating in and not originating in the neural crest. We have examined the sensitivity of cultured skin fibroblasts from patients with neurofibromatosis to several types of DNA damage. Fibroblasts in Dulbecco's modified Eagle's medium were plated at 10(2) to 2 X 10(4) cells per 75 cm2 tissue culture plates, and exposed to various doses of gamma radiation (leads to DNA scission), actinomycin D, or mitomycin C. Cells were reincubated for 15 to 40 daysmore » until surviving colonies exhibited greater than 30-50 cells. Plates were then stained with 1% methylene blue and the colonies counted, with surviving fraction determined relative to plating efficiency. Nine skin fibroblast cell strains from normal individuals were studied as controls. One neurofibromatosis (NF) cell strain, SB23, exhibited normal sensitivity to all three DNA-damaging agents studied in early (7-8) and middle (12-13) in vitro passage. Strain GM0622, on the other hand, exhibited normal sensitivity to the three DNA-damaging agents studied at early passage, but showed a significant decrease in survival after exposure to both gamma radiation (D0 = 106 rad) and actinomycin D (D0 = 0.024 mcg/ml) with increasing passage. Strain GM1639 exhibited decreased survival after actinomycin D exposure at early passage (D0 = 0.017 mcg/ml), with normal survival after exposure to gamma radiation and mitomycin C at the same passage.« less