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Sample records for modules m-7 m-15

  1. The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production.

    PubMed

    Tang, Wenxin; Tu, Lili; Yang, Xiyan; Tan, Jiafu; Deng, Fenglin; Hao, Juan; Guo, Kai; Lindsey, Keith; Zhang, Xianlong

    2014-04-01

    Fiber elongation is the key determinant of fiber quality and output in cotton (Gossypium hirsutum). Although expression profiling and functional genomics provide some data, the mechanism of fiber development is still not well understood. Here, a gene encoding a calcium sensor, GhCaM7, was isolated based on its high expression level relative to other GhCaMs in fiber cells at the fast elongation stage. The level of expression of GhCaM7 in the wild-type and the fuzzless/lintless mutant correspond to the presence and absence, respectively, of fiber initials. Overexpressing GhCaM7 promotes early fiber elongation, whereas GhCaM7 suppression by RNAi delays fiber initiation and inhibits fiber elongation. Reactive oxygen species (ROS) play important roles in early fiber development. ROS induced by exogenous hydrogen peroxide (H2 O2 ) and Ca(2+) starvation promotes early fiber elongation. GhCaM7 overexpression fiber cells show increased ROS concentrations compared with the wild-type, while GhCaM7 RNAi fiber cells have reduced concentrations. Furthermore, we show that H2 O2 enhances Ca(2+) influx into the fiber and feedback-regulates the expression of GhCaM7. We conclude that GhCaM7, Ca(2+) and ROS are three important regulators involved in early fiber elongation. GhCaM7 might modulate ROS production and act as a molecular link between Ca(2+) and ROS signal pathways in early fiber development.

  2. CCD Photometry of M15

    NASA Astrophysics Data System (ADS)

    Ruelas, A.; Sánchez, L. J.; Herrera, G.; Nigoche-Netro, A.

    2011-10-01

    We present CCD observations of the galactic globular cluster M15, in the B and V filters. The cluster was reasonably covered, except in its northern region where our observations present a gap. We obtained a Hertszprung-Russell (HR) diagram for each region observed, and later we produced a combined HR diagram containing more than 3000 stars. We generate a clean Colour Magnitude Diagram (CMD) and a Super Fiducial Line (SFL). Application of several methods and isochrone fitting leads us to obtain values for the metallicity [Fe/H]_{M15} ˜ -2.16±0.10, the reddening E(B-V)_{M15} ˜ 0.11±0.03, and a distance modulus of [(m-M)_0]_{M15}˜ 15.03.

  3. Hepatic Differentiation from Human Ips Cells Using M15 Cells.

    PubMed

    Umeda, Kahoko; Shiraki, Nobuaki; Kume, Shoen

    2016-01-01

    Here, we describe a procedure of human iPS cells differentiation into the definitive endoderm, further into albumin-expressing and albumin-secreting hepatocyte, using M15, a mesonephros- derived cell line. Approximately 90 % of human iPS cells differentiated into SOX17-positive definitive endoderm then approximately 50 % of cells became albumin-positive cells, and secreted ALB protein. This M15 feeder system for endoderm and hepatic differentiation is a simple and efficient method, and useful for elucidating molecular mechanisms for hepatic fate decision, and could represent an attractive approach for a surrogate cell source for pharmaceutical studies.

  4. Deep CCD photometry in globular clusters III. M15

    SciTech Connect

    Fahlman, G.G.; Richer, H.B.; Vandenberg, D.A.

    1985-06-01

    CCD photometry in U, B, and V is presented for a 5' x 3' field in the globular cluster M15. The location of the main sequence in the color-magnitude diagram is found here to be significantly bluer than previous studies have indicated. The luminosity function of the cluster is studied down to V = 22.8 (Mroughly-equal7.5) and shown to be consistent with a power-law mass function, n(M) = QM/sup -alpha/ with ..cap alpha.. = 2.5 +- 1.0, to the limit of our data. The field star population brighter than V = 21.5, is examined in some detail. There appears to be about 50% more stars belonging to the disk in the field as compared with the Bahcall-Soneira standard galaxy model. The reddening to the cluster is found to be E(B-V) = 0.11 +- 0.04 from nine bright field stars. A new value for the ultraviolet excess of the cluster main-sequence stars is obtained, delta(0.6) = 0.25 +- 0.02, and confirms the well-known fact that M15 is among the metal poorest of the globular clusters.

  5. Biotransformation studies of cresol red by Absidia spinosa M15.

    PubMed

    Kristanti, Risky Ayu; Fikri Ahmad Zubir, Meor Mohd; Hadibarata, Tony

    2016-05-01

    Cresol Red, a commercial dye that used widely to color nylon, wool, cotton, and polyacrylonitrile-modified nylon in the massive textile manufacture is toxic recalcitrant. Absidia spinosa M15, a novel fungal strain isolated from a tropical rain forest, was found to decolorize Cresol Red 65% within 30 d under agitation condition. UV-Vis spectroscopy, TLC analysis and mass spectra of samples after decolorization process in culture medium confirmed final decolorization of Cresol Red. Two metabolites were identified in the treated medium: benzeneacetic acid (tR 9.6 min and m/z 136) and benzoic acid (tR 5.7 min and m/z 122). Laccase showed the significant activity (133.8 U/L) in biomass obtained at the end of experiment demonstrates role of the enzyme in the decolorization process. PMID:26922501

  6. Biotransformation studies of cresol red by Absidia spinosa M15.

    PubMed

    Kristanti, Risky Ayu; Fikri Ahmad Zubir, Meor Mohd; Hadibarata, Tony

    2016-05-01

    Cresol Red, a commercial dye that used widely to color nylon, wool, cotton, and polyacrylonitrile-modified nylon in the massive textile manufacture is toxic recalcitrant. Absidia spinosa M15, a novel fungal strain isolated from a tropical rain forest, was found to decolorize Cresol Red 65% within 30 d under agitation condition. UV-Vis spectroscopy, TLC analysis and mass spectra of samples after decolorization process in culture medium confirmed final decolorization of Cresol Red. Two metabolites were identified in the treated medium: benzeneacetic acid (tR 9.6 min and m/z 136) and benzoic acid (tR 5.7 min and m/z 122). Laccase showed the significant activity (133.8 U/L) in biomass obtained at the end of experiment demonstrates role of the enzyme in the decolorization process.

  7. The stellar population in the core of M15

    NASA Technical Reports Server (NTRS)

    De Marchi, Guido; Paresce, Francesco

    1994-01-01

    The inner core of the globular cluster M15 within approximately 2 sec of the geometrical center has been explored with high-resolution images taken through several broad-band UV filter with the Faint Object Camera (FOC) on board the Hubble Space Telescope (HST). Approximately 210 stars in this region down to a 5 sigma detection limit of m(sub 220) = 21.5 were reliably identified and located on a UV - U color magnitude diagram for the first time. A majority of stars (about 70% of the total) observed this way lie above the expected main-sequence turn-off of this cluster and below the sparsely populated horizontal branch. The extension of the main sequence above the turn-off separates this population in two roughly equal components situated to the right and left of this line. Most of the former must be classical blue stragglers while the rest belong to a new, as yet unidentified, population of very blue stars. Possibilities include, but are not restricted to, well-mixed single stars, subdwarfs, and helium white dwarfs. Similar objects are also found just outside the core out to approximately 6 sec from the center, but the brighter, presumably more massive ones, are sharply confined to the core itself. The measured excess of bright blue stars and the relative deficiency of bright red giants in the core are consistent with the blue inward color gradient measured from the ground and imply that dynamical evolution can significantly affect the stellar population in the very dense central regions of a high-concentration globular cluster like M15.

  8. Epidemic Plasmid Carrying blaCTX-M-15 in Klebsiella penumoniae in China

    PubMed Central

    Zhuo, Chao; Li, Xiao-qiang; Zong, Zhi-yong; Zhong, Nan-Shan

    2013-01-01

    Objective To investigate the local epidemiology of Klebsiella penumoniae carrying blaCTX-M-15 in southern China and to characterize the genetic environment of blaCTX-M-15. Methods PCR and DNA sequencing were used to detect and characterize the genetic contexts of blaCTX-M-15. The clonal relatedness of isolates carrying blaCTX-M-15 was determined by pulse-field gel electrophoresis. Conjugative plasmids carrying blaCTX-M-15 were obtained by mating and were further subject to restriction analysis and replicon typing. Results A total of 47CTX-M-15 ESBL-producing isolates of K. pneumoniae were collected from nine hospitals in China from October 2007 to October 2008. Isolates were clustered into various clonal groups. The local spread of blaCTX-M-15 was mainly mediated by one major conjugative plasmid as determined by S1-PFGE and restriction analysis. A 90-kb plasmid belonging to incompatible group FII was the major carrier of blaCTX-M-15 in K. pneumoniae. Except blaTEM-1, the resistance genes such as blaSHV, blaDHA-1, blaOXA-1, qnrB, qnrS, aac(3)-II, and aac(6′)-Ib were not found in the plasmid. In the comparing of conjugative gene sequence, it is 100% identical with the plasmid pKF3–94, which was found in K. pneumonia from Zhejiang province of china previously. Conclusions blaCTX-M-15 was prevalent in K. pneumonia of southern China. The dissemination of blaCTX-M-15 appeared to be due to the horizontal transfer of a 90-kb epidemic plasmid. PMID:23382815

  9. Phosphate-modified analogues of m(7)GTP and m(7)Gppppm(7)G-Synthesis and biochemical properties.

    PubMed

    Ziemniak, Marcin; Kowalska, Joanna; Lukaszewicz, Maciej; Zuberek, Joanna; Wnek, Katarzyna; Darzynkiewicz, Edward; Jemielity, Jacek

    2015-09-01

    The synthesis and biochemical properties of 17 new mRNA cap analogues are reported. Six of these nucleotides are m(7)GTP derivatives, whereas 11 are 'two headed' tetraphosphate dinucleotides based on a m(7)Gppppm(7)G structure. The compounds contain either a boranophosphate or phosphorothioate moiety in the nucleoside neighbouring position(s) and some of them possess an additional methylene group between β and γ phosphorus atoms. The compounds were prepared by divalent metal chloride-mediated coupling of an appropriate m(7)GMP analogue with a given P(1),P(2)-di(1-imidazolyl) derivative. The analogues were evaluated as tools for studying cap-dependent processes in a number of biochemical assays, including determination of affinity to eukaryotic initiation factor eIF4E, susceptibility to enzymatic hydrolysis, and translational efficiency in vitro. The results indicate that modification in the phosphate chain can increase binding to cap-interacting proteins and provides higher resistance to degradation. Furthermore, modified derivatives of m(7)GTP were found to be potent inhibitors of cap-dependent translation in cell free systems.

  10. Genetic features of CTX-M-15-producing Acinetobacter baumannii from Haiti.

    PubMed

    Potron, Anaïs; Munoz-Price, L Silvia; Nordmann, Patrice; Cleary, Timothy; Poirel, Laurent

    2011-12-01

    Acinetobacter baumannii isolates T23, W35, and H1 were isolated from three patients who had been injured in the Haiti earthquake in January 2010. Those isolates, corresponding to two distinct clones, were identified as extended-spectrum β-lactamase (ESBL) producers and found to be bla(CTX-M-15)-positive. That ESBL gene was associated with ISEcp1, involved in its acquisition by a one-ended transposition mechanism. In all isolates, the ISEcp1-bla(CTX-M-15) compound transposon was apparently chromosomally located.

  11. German field test results on methanol fuels m100 and m15

    SciTech Connect

    Wolff, H.C.; Seiden, G.H.; Bandel, J.P.

    1983-01-01

    From 1979 to 1982, 22 industrial companies (including 8 oil companies and 8 engine manufacturers) and 15 scientific institutes investigated the use of methanol fuels m15 and m100 in field tests within the scope of a 110-million-Deutsche-mark program financially supported by the federal ministry for research and technology of the Federal Republic of Germany. Thirty stations for m15 and 15 for m100 were set up across the country, and 989 vehicles were tested in day-to-day driving. One result may be stated definitely: the production and operation of m15 vehicles are technically possible. A prerequisite would be setting up a comprehensive m15 station network. The technical preconditions are known. M100 demonstrated a high degree of thermal efficiency in private automobile engines and favorable exhaust gas emission values. Optimization of the engines is currently in progress. The lubricating problems (wear in cylinders and deposits in the inlets systems) have not been satisfactorily solved yet. The m100 engines for commercial vehicles and buses were developed from diesel engines. They have attained the same high degree of thermal efficiency as diesel engines. The exhaust gas emission is free of soot and is favorable with respect to nitrogen oxides content. The wear problems with buses in city traffic have been overcome.

  12. CTX-M-15-producing Morganella morganii from Hôpital Principal de Dakar, Senegal.

    PubMed

    Diene, S M; Fenollar, F; Fall, B; Sow, K; Niang, B; Samba Ba, P; Wade, B; Raoult, D; Rolain, J-M

    2014-03-01

    We report the detection and molecular characterization of extended spectrum β-lactamases in a series of 112 clinical isolates of Enterobacteriaceae from the Hôpital Principal de Dakar, Senegal, including five CTX-M-15-producing Morganella morganii isolates, which are reported for the first time in this country.

  13. The central mass and mass-to-light profile of the Galactic globular cluster M15

    NASA Astrophysics Data System (ADS)

    den Brok, Mark; van de Ven, Glenn; van den Bosch, Remco; Watkins, Laura

    2014-02-01

    We analyse line-of-sight velocity and proper motion data of stars in the Galactic globular cluster M15 using a new method to fit dynamical models to discrete kinematic data. Our fitting method maximizes the likelihood for individual stars and, as such, does not suffer the same loss of spatial and velocity information incurred when spatially binning data or measuring velocity moments. In this paper, we show that the radial variation in M15 of the mass-to-light ratio is consistent with previous estimates and theoretical predictions, which verifies our method. Our best-fitting axisymmetric Jeans models do include a central dark mass of ˜2 ± 1 × 103 M⊙, which can be explained by a high concentration of stellar remnants at the cluster centre. This paper shows that, from a technical point of view and with current computing power, spatial binning of data is no longer necessary. This not only leads to more accurate fits, but also avoids biased mass estimates due to the loss of resolution. Furthermore, we find that the mass concentration in M15 is significantly higher than previously measured, and is in close agreement with theoretical predictions for core-collapsed globular clusters without a central intermediate-mass black hole.

  14. Statement of Basis: Building M7-505 Treatment Tank SWMU 039

    NASA Technical Reports Server (NTRS)

    Starr, Andrew Scott

    2015-01-01

    The Statement of Basis (SB) has been developed to inform and give the public an opportunity to comment on a proposed remedy to address contamination at the Building M7-505 Treatment Tank (M7-505) site.

  15. Fabry-Perot Velocities For Globular Cluster Stars: M15, M3, and M4

    NASA Astrophysics Data System (ADS)

    Gebhardt, Karl; Pryor, Carlton; Williams, T. B.; Hesser, James E.

    1994-12-01

    We are engaged in a continuing project that uses an imaging Fabry-Perot spectrophotometer on the 3.6-m CFHT and the 4 m and 1.5 m telescopes at CTIO to measure both absorption-line integrated-light velocity maps for globular clusters and velocities for large samples of cluster stars (Gebhardt, K., Pryor, C., Williams, T.B., & Hesser, J.E. 1994, AJ, 107, 2067; Gebhardt, et al. 1994, submitted). Our goals are to study such kinematic properties as rotation and the number of high-velocity stars, and to use the velocity dispersion profiles to determine non-parametric mass profiles using the technique of Gebhardt & Fischer (1995, January AJ). Here we present radial velocities with accuracies of 1--5 \\kms\\ for about 600 stars in the cusp cluster M15, 400 stars in the centrally-concentrated cluster M3, and 1900 stars in the nearby cluster M4. The M15 dataset contains three times more stellar velocities throughout the whole cluster and five times more in the inner 10'' than the previous study of Gebhardt & Fischer. This larger sample continues to show no evidence for a central cusp in the velocity dispersion profile. The M4 data constitute one of the largest radial velocity samples yet obtained for a globular cluster. The stars in the sample range from magnitude V=10 to V=18.5. V=18.5 is 2.5 magnitudes below the main-sequence turn-off, so these data are valuable first-epoch information for determining the main-sequence binary fraction. We present two-dimensional velocity maps, velocity dispersion profiles, and radial mass profiles for the three clusters.

  16. THE SWIFT UVOT STARS SURVEY. II. RR LYRAE STARS IN M3 AND M15

    SciTech Connect

    Siegel, Michael H.; Porterfield, Blair L.; Balzer, Benjamin G.; Hagen, Lea M. Z. E-mail: blp14@psu.edu E-mail: lea.zernow.hagen@gmail.com

    2015-10-15

    We present the first results of a near-ultraviolet (NUV) survey of RR Lyrae stars from the Ultraviolet Optical Telescope (UVOT) on board the Swift Gamma-ray Burst Mission. It is well-established that RR Lyrae stars have large amplitudes in the far- and near-ultraviolet. We have used UVOT’s unique wide-field NUV imaging capability to perform the first systematic NUV survey of variable stars in the Galactic globular clusters M3 and M15. We identify 280 variable stars, comprised of 275 RR Lyrae, 2 anomalous Cepheids, 1 classical Cepheid, 1 SX Phoenicis star, and 1 possible long-period or irregular variable. Only two of these are new discoveries. We compare our results to previous investigations and find excellent agreement in the periods with significantly larger amplitudes in the NUV. We map out, for the first time, an NUV Bailey diagram from globular clusters, showing the usual loci for fundamental mode RRab and first overtone RRc pulsators. We show the unique sensitivity of NUV photometry to both the temperatures and the surface gravities of RR Lyrae stars. Finally, we show evidence of an NUV period–metallicity–luminosity relationship. Future investigations will further examine the dependence of NUV pulsation parameters on metallicity and Oosterhoff classification.

  17. Rapid Emergence and Clonal Dissemination of CTX-M-15-Producing Salmonella enterica Serotype Virchow, South Korea.

    PubMed

    Kim, Jin Seok; Yun, Young-Sun; Kim, Soo Jin; Jeon, Se-Eun; Lee, Deog-yong; Chung, Gyung Tae; Yoo, Cheon-Kwon; Kim, Junyoung

    2016-01-01

    The prevalence of cefotaxime-resistant Salmonella enterica serotype Virchow has dramatically increased in South Korea since the first isolation in 2011. Of 68 isolates collected over 10 years, 28 cefotaxime-resistant isolates harbored the bla(CTX-M-15) extended-spectrum β-lactamase gene and were closely related genetically, demonstrating the clonal dissemination of CTX-M-15-producing Salmonella Virchow in South Korea.

  18. Growth characteristics of primary M7C3 carbide in hypereutectic Fe-Cr-C alloy.

    PubMed

    Liu, Sha; Zhou, Yefei; Xing, Xiaolei; Wang, Jibo; Ren, Xuejun; Yang, Qingxiang

    2016-01-01

    The microstructure of the hypereutectic Fe-Cr-C alloy is observed by optical microscopy (OM). The initial growth morphology, the crystallographic structure, the semi-molten morphology and the stacking faults of the primary M7C3 carbide are observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in-suit growth process of the primary M7C3 carbide was observed by confocal laser microscope (CLM). It is found that the primary M7C3 carbide in hypereutectic Fe-Cr-C alloy is irregular polygonal shape with several hollows in the center and gaps on the edge. Some primary M7C3 carbides are formed by layers of shell or/and consist of multiple parts. In the initial growth period, the primary M7C3 carbide forms protrusion parallel to {} crystal planes. The extending and revolving protrusion forms the carbide shell. The electron backscattered diffraction (EBSD) maps show that the primary M7C3 carbide consists of multiple parts. The semi-molten M7C3 carbide contains unmelted shell and several small-scale carbides inside, which further proves that the primary M7C3 carbide is not an overall block. It is believed that the coalescence of the primary M7C3 carbides is ascribed to the growing condition of the protrusion and the gap filling process. PMID:27596718

  19. Growth characteristics of primary M7C3 carbide in hypereutectic Fe-Cr-C alloy.

    PubMed

    Liu, Sha; Zhou, Yefei; Xing, Xiaolei; Wang, Jibo; Ren, Xuejun; Yang, Qingxiang

    2016-09-06

    The microstructure of the hypereutectic Fe-Cr-C alloy is observed by optical microscopy (OM). The initial growth morphology, the crystallographic structure, the semi-molten morphology and the stacking faults of the primary M7C3 carbide are observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in-suit growth process of the primary M7C3 carbide was observed by confocal laser microscope (CLM). It is found that the primary M7C3 carbide in hypereutectic Fe-Cr-C alloy is irregular polygonal shape with several hollows in the center and gaps on the edge. Some primary M7C3 carbides are formed by layers of shell or/and consist of multiple parts. In the initial growth period, the primary M7C3 carbide forms protrusion parallel to {} crystal planes. The extending and revolving protrusion forms the carbide shell. The electron backscattered diffraction (EBSD) maps show that the primary M7C3 carbide consists of multiple parts. The semi-molten M7C3 carbide contains unmelted shell and several small-scale carbides inside, which further proves that the primary M7C3 carbide is not an overall block. It is believed that the coalescence of the primary M7C3 carbides is ascribed to the growing condition of the protrusion and the gap filling process.

  20. Growth characteristics of primary M7C3 carbide in hypereutectic Fe-Cr-C alloy

    NASA Astrophysics Data System (ADS)

    Liu, Sha; Zhou, Yefei; Xing, Xiaolei; Wang, Jibo; Ren, Xuejun; Yang, Qingxiang

    2016-09-01

    The microstructure of the hypereutectic Fe-Cr-C alloy is observed by optical microscopy (OM). The initial growth morphology, the crystallographic structure, the semi-molten morphology and the stacking faults of the primary M7C3 carbide are observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in-suit growth process of the primary M7C3 carbide was observed by confocal laser microscope (CLM). It is found that the primary M7C3 carbide in hypereutectic Fe-Cr-C alloy is irregular polygonal shape with several hollows in the center and gaps on the edge. Some primary M7C3 carbides are formed by layers of shell or/and consist of multiple parts. In the initial growth period, the primary M7C3 carbide forms protrusion parallel to {} crystal planes. The extending and revolving protrusion forms the carbide shell. The electron backscattered diffraction (EBSD) maps show that the primary M7C3 carbide consists of multiple parts. The semi-molten M7C3 carbide contains unmelted shell and several small-scale carbides inside, which further proves that the primary M7C3 carbide is not an overall block. It is believed that the coalescence of the primary M7C3 carbides is ascribed to the growing condition of the protrusion and the gap filling process.

  1. Growth characteristics of primary M7C3 carbide in hypereutectic Fe-Cr-C alloy

    PubMed Central

    Liu, Sha; Zhou, Yefei; Xing, Xiaolei; Wang, Jibo; Ren, Xuejun; Yang, Qingxiang

    2016-01-01

    The microstructure of the hypereutectic Fe-Cr-C alloy is observed by optical microscopy (OM). The initial growth morphology, the crystallographic structure, the semi-molten morphology and the stacking faults of the primary M7C3 carbide are observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in-suit growth process of the primary M7C3 carbide was observed by confocal laser microscope (CLM). It is found that the primary M7C3 carbide in hypereutectic Fe-Cr-C alloy is irregular polygonal shape with several hollows in the center and gaps on the edge. Some primary M7C3 carbides are formed by layers of shell or/and consist of multiple parts. In the initial growth period, the primary M7C3 carbide forms protrusion parallel to {} crystal planes. The extending and revolving protrusion forms the carbide shell. The electron backscattered diffraction (EBSD) maps show that the primary M7C3 carbide consists of multiple parts. The semi-molten M7C3 carbide contains unmelted shell and several small-scale carbides inside, which further proves that the primary M7C3 carbide is not an overall block. It is believed that the coalescence of the primary M7C3 carbides is ascribed to the growing condition of the protrusion and the gap filling process. PMID:27596718

  2. The Discovery of a Second Luminous Low Mass X-ray Binary in the Globular Cluster M15

    NASA Technical Reports Server (NTRS)

    White, Nicholas E.; Angelini, Lorella

    2001-01-01

    We report an observation by the Chandra X-ray Observatory of 4U2127+119, the X-ray source identified with the globular cluster M15. The Chandra observation reveals that 4U2127+119 is in fact two bright sources, separated by 2.7". One source is associated with AC21 1, the previously identified optical counterpart to 4U2127+119, a low mass X-ray binary (LMXB). The second source, M15-X2, is coincident with a 19th U magnitude blue star that is 3.3" from the cluster core. The Chandra count rate of M15-X2 is 2.5 times higher than that of AC211. Prior to the 0.5" imaging capability of Chandra the presence of two so closely separated bright sources would not have been resolved, The optical counterpart, X-ray luminosity and spectrum of M15-X2 are consistent with it also being an LMXB system. This is the first time that two LMXBS have been seen to be simultaneously active in a globular cluster. The discovery of a second active LMXB in M15 solves a long standing puzzle where the properties of AC211 appear consistent with it being dominated by an extended accretion disk corona, and yet 4U2127+119 also shows luminous X-ray bursts requiring that the neutron star be directly visible. The resolution of 4U2127+119 into two sources suggests that the X-ray bursts did not come from AC211, but rather from M15X2. We discuss the implications of this discovery for understanding the origin and evolution of LMXBs in GCs as well as X-ray observations of globular clusters in nearby galaxies.

  3. Replicon typing of plasmids carrying blaCTX-M-15 among Enterobacteriaceae isolated at the environment, livestock and human interface.

    PubMed

    Zurfluh, Katrin; Glier, Melinda; Hächler, Herbert; Stephan, Roger

    2015-07-15

    One of the currently most important antibiotic resistance mechanisms in Enterobacteriaceae is based on the production of ESBL enzymes that inactivate β-lactam antibiotics including cephalosporins and monobactams by hydrolyzing their β-lactam ring. In humans, the most prevalent ESBL enzyme type is encoded by blaCTX-M-15. CTX-M-15 producing enterobacterial strains were also frequently isolated from environmental samples including surface water and freshwater fish. Plasmids, which can be grouped in different plasmid incompatibility types, play a key role in the horizontal spread of these multidrug resistance genes. The purpose of this study was to investigate the diversity of plasmids that carry blaCTX-M-15 genes among Enterobacteriaceae isolated at the environment, livestock and human interface. In total, 81 blaCTX-M-15-harboring isolates collected between 2009 and 2014 were tested for its ability to transfer blaCTX-M-15 by conjugation. These plasmids were further typed. Transfer of a single blaCTX-M-15-harboring plasmid was observed in 32 (39.5%) of the isolates. The most frequent replicon types detected among these plasmids are IncF-type plasmids (n=12) (mostly multi replicon plasmids with a combination of following replicons: IncFII, IncFIA and IncFIB), followed by IncI1 (n=8), IncK (n=3) and IncR (n=1). A noticeable number of plasmids (n=8) could not be assigned to any of the tested replicon types. Knowledge about the plasmid types circulating in bacterial populations is indispensable for understanding epidemiological dynamics and for establishing intervention strategies to stop further dissemination of particular plasmids.

  4. The Discovery of a Second Luminous Low-Mass X-Ray Binary in the Globular Cluster M15

    NASA Technical Reports Server (NTRS)

    White, Nicholas E.; Angelini, Lorella

    2001-01-01

    We report an observation by the Chandra X-Ray Observatory of 4U 2127+119, the X-ray source identified with the globular cluster M15. The Chandra observation reveals that 4U 2127+119 is in fact two bright sources, separated by 2.7 arcsec. One source is associated with AC 211, the previously identified optical counterpart to 4U 2127+119, a low-mass X-ray binary (LMXB). The second source, M15 X-2, is coincident with a 19th U magnitude blue star that is 3.3 arcsec from the cluster core. The Chandra count rate of M15 X-2 is 2.5 times higher than that of AC 211. Prior to the 0.5 arcsec imaging capability of Chandra, the presence of two so closely separated bright sources would not have been resolved. The optical counterpart, X-ray luminosity, and spectrum of M15 X-2 are consistent with it also being an LMXB system. This is the first time that two LMXBs have been seen to be simultaneously active in a globular cluster. The discovery of a second active LMXB in M15 solves a long-standing puzzle where the properties of AC 211 appear consistent with it being dominated by an extended accretion disk corona, and yet 4U 2127+119 also shows luminous X-ray bursts requiring that the neutron star be directly visible. The resolution of 4U 2127+119 into two sources suggests that the X-ray bursts did not come from AC 211 but rather from M15 X-2. We discuss the implications of this discovery for understanding the origin and evolution of LMXBs in globular clusters as well as X-ray observations of globular clusters in nearby galaxies.

  5. Changing Epidemiology of Extended-Spectrum β-Lactamases in Argentina: Emergence of CTX-M-15

    PubMed Central

    Sennati, S.; Santella, G.; Di Conza, J.; Pallecchi, L.; Pino, M.; Ghiglione, B.; Rossolini, G. M.; Radice, M.

    2012-01-01

    A multicenter survey, carried out in 2010 in Argentina, showed an increased prevalence of extended-spectrum β-lactamase (ESBL)-producing enterobacteria, with some changes in the molecular epidemiology of circulating ESBLs. While enzymes of the CTX-M-2 group remain endemic, the emergence of CTX-M-15 and of enzymes of the CTX-M-8 and CTX-M-9 groups was observed. The CTX-M-15-positive isolates represented 40% of CTX-M producers and included representatives of Escherichia coli ST131 and Klebsiella pneumoniae ST11. PMID:22908156

  6. Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy.

    PubMed

    Cerquetti, M; Giufrè, M; García-Fernández, A; Accogli, M; Fortini, D; Luzzi, I; Carattoli, A

    2010-10-01

    Quinolone and β-lactam resistance mechanisms and clonal relationships were characterized among Escherichia coli isolates resistant to ciprofloxacin and extended-spectrum cephalosporins associated with human extra-intestinal infections in Rome. The E. coli. ST131 clone was found to be prevalent. This clone invariably carried a specific pattern of substitutions in the topoisomerase genes and all isolates but one produced CTX-M-15. One ST131 isolate produced SHV-12. The new ST131 variant described here is of particular concern because it combines fluoroquinolone resistance and chromosomally encoded CTX-M-15.

  7. The Discovery of a Second Luminous Low Mass X-Ray Binary System in the Globular Cluster M15

    NASA Technical Reports Server (NTRS)

    White, Nicholas E.; Angelini, Lorella

    2001-01-01

    Using the Chandra X-ray Observatory we have discovered a second bright X-ray source in the globular cluster M15 that is 2.7" to the west of AC211, the previously known low mass X-ray binary (LMXB) in this system. Prior to the 0.5" imaging capability of Chandra this second source could not have been resolved from AC211. The luminosity and spectrum of this new source, which we call M15-X2, are consistent with it also being a LMXB system. This is the first time that two LMXBs have been seen to be simultaneously active in a globular cluster. The new source, M15-X2, is coincident with a 18th U magnitude very blue star. The discovery of a second LMXB in M15 clears up a long standing puzzle where the X-ray and optical properties of AC211 appear consistent with the central source being hidden behind an accretion disk corona, and yet also showed a luminous X-ray burst suggesting the neutron star is directly visible. This discovery suggests instead that the X-ray burst did not come from AC211, but rather from the newly discovered X-ray source. We discuss the implications of this discovery for X-ray observations of globular clusters in nearby galaxies.

  8. An insight into the biophysical characterization of different states of cefotaxime hydrolyzing β-lactamase 15 (CTX-M-15).

    PubMed

    Rehman, Md Tabish; Faheem, Mohd; Khan, Asad U

    2015-01-01

    Cefotaxime hydrolyzing β-lactamase-15 (CTX-M-15) is encoded by blaCTX-M-15 gene present on plasmid of various Gram-negative bacteria, such as E. coli, E. cloacae, K. pneumoniae, etc. The widespread dissemination of CTX-M-15 harboring bacteria in hospital as well as community settings is a universal threat as they are resistant to various clinically significant antibiotics. In order to gain an insight into the folding mechanism of CTX-M-15, we carried out pH-induced denaturation study by monitoring Trp fluorescence, far-UV circular dichroism (CD), and ANS fluorescence. We found that the pH-induced denaturation of CTX-M-15 was a three-step process with the accumulation of two stable folding intermediates (XI at pH 2.5 and XII at pH 1.5) in the folding pathway. The intermediates were further characterized by far-UV and near-UV CD analysis, Trp fluorescence, ANS fluorescence, three-dimensional fluorescence, acrylamide quenching, dynamic light scattering, and thermal denaturation studies. We found that XI state lacked tertiary structure but retained most of the secondary structure, its Trp residues were partially exposed to the solvent and its hydrophobic patches were highly accessible to ANS. On the other hand, a complete disruption of tertiary structure along with more than 50% loss in secondary structure was observed in XII state. We conclude that the XI state of CTX-M-15 at pH 2.5 had all the characteristics of a molten globule (MG) state, while its XII state at pH 1.5 was more similar to pre-molten globule (PMG) state. ANS fluorescence also showed that the binding of ANS in XII state was lower than that in the XI state. We propose that the accumulation of MG- and PMG-states was due to separation (at pH 2.5) and then unfolding (at pH 1.5) of the αβα-fold of CTX-M-15, respectively. PMID:24650131

  9. 20. Public Works Department Drawing 461M7 (1943), 'Sulphuric Acid Storage ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Public Works Department Drawing 461-M-7 (1943), 'Sulphuric Acid Storage System-Building 463 Details' - Mare Island Naval Shipyard, Acid Mixing Facility, California Avenue & E Street, Vallejo, Solano County, CA

  10. High prevalence of CTX-M-15-producing O25b-ST131 Escherichia coli clone in Bulgarian hospitals.

    PubMed

    Markovska, Rumyana; Schneider, Ines; Ivanova, Dobrinka; Keuleyan, Emma; Stoeva, Temenuga; Sredkova, Mariya; Markova, Boyka; Bojkova, Kalina; Gergova, Raina; Bauernfeind, Adolf; Mitov, Ivan

    2012-08-01

    According to the European Antimicrobial Resistance Surveillance System project results, Bulgaria has become one of the European countries with dramatically increasing rates of extended-spectrum beta-lactamase (ESBL) producers. The aim of this work was to investigate the epidemiology of ESBL-producing Escherichia coli clinical isolates in Bulgaria, collected from seven clinical centers in three towns, during two study periods: 2002-2003 and 2006-2009. For 193 ESBL-producing E. coli isolates random amplified polymorphic DNA (RAPD) analyses, phylogenetic typing, and screening for O25b-ST131 isolates were carried out. Antimicrobial susceptibility, ESBL-type and transferability of resistance determinants were analyzed. Four different ESBL-types, namely TEM-139, SHV-12, CTX-M-3, and CTX-M-15 were found. CTX-M-15 dominated, being found in 88% of the isolates. RAPD-typing revealed 35 types, among which type A dominated, comprising 65% of the isolates. Sixty-eight percent of the 193 isolates belonged to the O25b-ST131 clone, to the phylogenetic group B2, mostly showed RAPD-type A (92%) and were found in all participating hospitals. O25b-ST131 isolates predominantly produced CTX-M-15 (96%), and less SHV-12 (n=3) or TEM-139 (n=2). In conclusion, this study demonstrated for the first time the country-wide dissemination of a highly resistant B2 O25b-ST131 CTX-M-15 producing E. coli clone in Bulgaria.

  11. Characterization of Salmonella Isangi possessing a CTX-M15 ESBL associated with an outbreak in a US Hospital.

    PubMed

    Suleyman, Geehan; Perri, Mary; Vager, Dora; Samuel, Linoj; Zervos, Marcos J; Alangaden, George; Tibbetts, Robert J

    2016-07-01

    Over an approximately 50-day period in 2015, an outbreak of CTX-M-15 extended spectrum β-lactamase-(ESBL)-possessing Salmonella Isangi occurred among 19 adult surgical patients and one healthcare worker (HCW) at a large urban tertiary care hospital in the United States. A total of 45 S. Isangi isolates were isolated from stool (35), blood (4), urine (3), respiratory (2), and wound (1) cultures. Phenotypically, all but three isolates demonstrated resistance to ampicillin, ampicillin/sulbactam, ceftriaxone, and cefepime, and one isolate was resistant to ertapenem. Genotypically, a single CTX-M-15 ESBL was identified in all but three isolates by real-time PCR. Interestingly, two of the CTX-M-15 negative, susceptible isolates were isolated from a single patient who initially had a CTX-M positive, resistant strain. Isolates were clonally related, including both resistant and susceptible strains, as confirmed by pulse field gel electrophoresis (PFGE). This is the first case of a novel Salmonella outbreak at this hospital, and we believe it to be the first case of an S. Isangi serotype outbreak in the United States. PMID:27130476

  12. Low intestinal colonization of Escherichia coli clone ST131 producing CTX-M-15 in Jordanian infants.

    PubMed

    Badran, E F; Qamer Din, R A; Shehabi, A A

    2016-02-01

    Over a period of 3 years' study (2012-2014), a total of 518 faecal samples were collected and cultured to isolate Escherichia coli. Of these, 338 (65.3%) E. coli isolates were recovered from infants, and 142/338 (42%) were multidrug-resistant (MDR) to ≥ 3 drug classes using the antimicrobial susceptibility disc diffusion method. A total of 125/142 (88%) of E. coli isolates were extended-spectrum β-lactamase (ESBL) producers. blaCTX-M-15 types were observed in 80/125 (64%) of the isolates, and 60/80 (75%) were positive for blaCTX-M-15. Out of 338 E. coli isolates, 9 (2.6%) were positive for ST131/O25b clone and each isolate was associated with several plasmids of different sizes (1-21.2 kb). The identities of these nine isolates were confirmed by sequencing for presence of pabB (347 bp) and trpA (427 bp) genes. This study demonstrates low prevalence rate of the highly virulent E. coli ST131 clone producing blaCTX-M-15 in the intestines of Jordanian infants.

  13. blaCTX-M-15-carrying F2:A-:B- plasmid in Escherichia coli from cattle milk in Tunisia.

    PubMed

    Grami, Raoudha; Dahmen, Safia; Mansour, Wejdene; Mehri, Wahib; Haenni, Marisa; Aouni, Mahjoub; Madec, Jean-Yves

    2014-08-01

    Extended-spectrum β-lactamases (ESBL) are widespread enzymes in animals, and the risk of transmission of ESBL genes to humans has become a major issue. In Tunisia, recent data showed a high prevalence of ESBL-producing Escherichia coli isolates in healthy animals, mostly in chickens. In this study, we report the first data on ESBL in diseased Tunisian animals (chickens and cattle), highlighting a major difference in ESBL prevalence in the infectious versus noninfectious E. coli flora. Interestingly, the only ESBL producer was an ST10 E. coli from a cattle, and not from chicken. Moreover, this E. coli isolate harbored the bla(CTX-M-15) gene on an F2:A-:B- plasmid, a combination frequently found in humans. This plasmid was also highly similar to a bla(CTX-M-15) F2:A-:B- plasmid recently reported in cattle in France. Altogether, this study is also the first report of the bla(CTX-M-15) gene in food animals in Tunisia, and, to our best knowledge, the first report of an ESBL producer in cattle in Africa. Since this plasmid was recognized in cattle in France and worldwide in humans, the question of its origin in Tunisian cattle is open. The detection of ESBL producers in milk in Tunisia may also constitute a risk of ESBL transmission from animals to humans through food consumption.

  14. Significant foreshock activities of M>7.5 earthquakes in the Kuril subduction zone

    NASA Astrophysics Data System (ADS)

    Harada, T.; Yokoi, S.; Satake, K.

    2014-12-01

    In the Kuril subduction zone, some M>7.5 earthquakes are accompanied by significant foreshock activities, providing a good opportunity to understand the characteristics of foreshocks for large interplate events such as occur along the Japan Trench and Nankai Trough etc. Some preliminary results from our examination of the foreshock sequences are as follows. Relocated foreshocks tend to migrate with time toward the trench axis. Foreshock distributions of the interplate earthquakes do not overlap with the large coseismic slips (asperities) of the mainshocks. Foreshocks of the 2007 northern Kuril outer-rise event, however, were distributed on the entire rupture area. Foreshock sequences seem to be limited in the regions where the background seismicity rates are relatively high. The foreshock activities were found in the examination of the space-time pattern of M>7 events along the northern Japan to Kuril trench since 1913 (e.g. Harada, Satake, and Ishibashi, 2011:AGU, 2012:AOGS). The large earthquakes preceded by active foreshock sequences are: the 2006 (M8.3), 2007 (M8.1) offshore Simushir earthquakes, the 1963 (M8.5), 1991 (M7.6), 1995 (M7.9) offshore Urup events, the 1978 (M7.8) offshore Iturup events, the 1969 (M8.2) offshore Shikotan event. In contrast, M>7.5 interplate earthquakes offshore Hokkaido (1952 (M8.1), 1973 (M7.8), 2003 (M8.1)) and intraslab earthquakes (1958 (M8.3), 1978 (M7.8), 1993 (M7.6), 1994 (M8.3)) had few or no foreshocks. In the examination of the active foreshocks, we relocated foreshocks by the Modified JHD method (Hurukawa, 1995), compared relocated foreshock areas with mainshock coseismic slip distributions estimated by the teleseismic body-wave inversion (Kikuchi and Kanamori, 2003), and examined the relation between active foreshock sequences and regional background seismicity. This study was supported by the MEXT's "New disaster mitigation research project on Mega thrust earthquakes around Nankai/Ryukyu subduction zones".

  15. Dosimetric characterization of the M-15 high-dose-rate Iridium-192 brachytherapy source using the AAPM and ESTRO formalism.

    PubMed

    Ho Than, Minh-Tri; Munro Iii, John J; Medich, David C

    2015-05-08

    The Source Production & Equipment Co. (SPEC) model M-15 is a new Iridium-192 brachytherapy source model intended for use as a temporary high-dose-rate (HDR) brachytherapy source for the Nucletron microSelectron Classic afterloading system. The purpose of this study is to characterize this HDR source for clinical application by obtaining a complete set of Monte Carlo calculated dosimetric parameters for the M-15, as recommended by AAPM and ESTRO, for isotopes with average energies greater than 50 keV. This was accomplished by using the MCNP6 Monte Carlo code to simulate the resulting source dosimetry at various points within a pseudoinfinite water phantom. These dosimetric values next were converted into the AAPM and ESTRO dosimetry parameters and the respective statistical uncertainty in each parameter also calculated and presented. The M-15 source was modeled in an MCNP6 Monte Carlo environment using the physical source specifications provided by the manufacturer. Iridium-192 photons were uniformly generated inside the iridium core of the model M-15 with photon and secondary electron transport replicated using photoatomic cross-sectional tables supplied with MCNP6. Simulations were performed for both water and air/vacuum computer models with a total of 4 × 109 sources photon history for each simulation and the in-air photon spectrum filtered to remove low-energy photons belowδ = 10 keV. Dosimetric data, including D·(r,θ), gL(r), F(r,θ), φan(r), and φ-an, and their statistical uncertainty were calculated from the output of an MCNP model consisting of an M-15 source placed at the center of a spherical water phantom of 100 cm diameter. The air kerma strength in free space, SK, and dose rate constant, Λ, also was computed from a MCNP model with M-15 Iridium-192 source, was centered at the origin of an evacuated phantom in which a critical volume containing air at STP was added 100 cm from the source center. The reference dose rate, D·(r0,θ0) ≡ D· (1cm

  16. Systematic monitoring and evaluation of M7 scanner performance and data quality

    NASA Technical Reports Server (NTRS)

    Stewart, S.; Christenson, D.; Larsen, L.

    1974-01-01

    An investigation was conducted to provide the information required to maintain data quality of the Michigan M7 Multispectral scanner by systematic checks on specific system performance characteristics. Data processing techniques which use calibration data gathered routinely every mission have been developed to assess current data quality. Significant changes from past data quality are thus identified and attempts made to discover their causes. Procedures for systematic monitoring of scanner data quality are discussed. In the solar reflective region, calculations of Noise Equivalent Change in Radiance on a permission basis are compared to theoretical tape-recorder limits to provide an estimate of overall scanner performance. M7 signal/noise characteristics are examined.

  17. 78 FR 22269 - International Conference on Harmonisation; Draft Guidance on M7 Assessment and Control of DNA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-15

    ... M7 Assessment and Control of DNA Reactive (Mutagenic) Impurities in Pharmaceuticals To Limit... ``M7 Assessment and Control of DNA Reactive (Mutagenic) Impurities in Pharmaceuticals to Limit... and Control of DNA Reactive (Mutagenic) Impurities in Pharmaceuticals to Limit Potential...

  18. The Absolute Age of the Globular Cluster M15 Using Near-infrared Adaptive Optics Images from PISCES/LBT.

    NASA Astrophysics Data System (ADS)

    Monelli, M.; Testa, V.; Bono, G.; Ferraro, I.; Iannicola, G.; Fiorentino, G.; Arcidiacono, C.; Massari, D.; Boutsia, K.; Briguglio, R.; Busoni, L.; Carini, R.; Close, L.; Cresci, G.; Esposito, S.; Fini, L.; Fumana, M.; Guerra, J. C.; Hill, J.; Kulesa, C.; Mannucci, F.; McCarthy, D.; Pinna, E.; Puglisi, A.; Quiros-Pacheco, F.; Ragazzoni, R.; Riccardi, A.; Skemer, A.; Xompero, M.

    2015-10-01

    We present deep near-infrared J, {K}{{s}} photometry of the old, metal-poor Galactic globular cluster M15 obtained with images collected with the LUCI1 and PISCES cameras available at the Large Binocular Telescope (LBT). We show how the use of First Light Adaptive Optics (FLAO) system coupled with the PISCES camera allows us to improve the limiting magnitude by ˜2 mag in {K}{{s}}. By analyzing archival Hubble Space Telescope data, we demonstrate that the quality of the LBT/PISCES color-magnitude diagram is fully comparable with analogous space-based data. The smaller field of view is balanced by the shorter exposure time required to reach a similar photometric limit. We investigated the absolute age of M15 by means of two methods: (i) by determining the age from the position of the main-sequence turnoff (MSTO), and (ii) by the magnitude difference between the MSTO and the well-defined knee detected along the faint portion of the MS. We derive consistent values of the absolute age of M15, that is, 12.9 ± 2.6 Gyr and 13.3 ± 1.1 Gyr, respectively. Observations were carried out using the Large Binocular Telescope at Mount Graham, AZ. The LBT is an international collaboration among institutions in the United States, Italy, and Germany. LBT Corporation partners are the University of Arizona on behalf of the Arizona university system; Istituto Nazionale di Astrofisica, Italy; LBT Beteiligungsgesellschaft, Germany, representing the Max-Planck Society, the Astrophysical Institute Potsdam, and Heidelberg University; the Ohio State University; and the Research Corporation, on behalf of the University of Notre Dame, University of Minnesota, and University of Virginia.

  19. CTX-M-15-type extended-spectrum beta-lactamases-producing Escherichia coli from wild birds in Germany.

    PubMed

    Guenther, Sebastian; Grobbel, Mirjam; Beutlich, Janine; Bethe, Astrid; Friedrich, Nicole D; Goedecke, Andreas; Lübke-Becker, Antina; Guerra, Beatriz; Wieler, Lothar H; Ewers, Christa

    2010-10-01

    The isolation of Escherichia coli from wild birds in Germany revealed the occurrence of four CTX-M-15-producing strains from four different birds (2.3% of 172 isolates). CTX-M producers were recovered from two Eurasian Blackbirds, one Rock Pigeon and a Greater White-fronted Goose. All CTX-M-producing E. coli revealed a clonal relationship as determined by pulsed-field gel electrophoresis (PFGE) and were assigned to multilocus sequence type (ST) 648. Our findings suggest the emergence of a new clone with epidemiological importance and strengthen the role of wild bird species other than waterfowl as possible reservoirs of ESBL-producing Enterobacteriaceae. PMID:23766249

  20. 18. PWD Drawing 1123915 (814M7) (1945), 'Machinery Cleaning Bldg., Bldg ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. PWD Drawing 11239-15 (814-M-7) (1945), 'Machinery Cleaning Bldg., Bldg No. 814, Fire Protection Automatic CO2 and Tank Cover Control' - Mare Island Naval Shipyard, Chemical Cleaning Facility, North of Fourteenth Street, between California & Railroad Avenue, Vallejo, Solano County, CA

  1. CATS-ISS_L1B_N-M7.1-V2-07

    Atmospheric Science Data Center

    2016-10-24

    CATS-ISS_L1B_N-M7.1-V2-07 The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive ... Injection Heights Cloud Vertical Extent Aerosol Vertical Extent Volume Depolarization Ratio Profiles Attenuated ...

  2. CATS-ISS_L1B_D-M7.1-V2-07

    Atmospheric Science Data Center

    2016-10-25

    CATS-ISS_L1B_D-M7.1-V2-07 The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive ... Injection Heights Cloud Vertical Extent Aerosol Vertical Extent Volume Depolarization Ratio Profiles Attenuated ...

  3. CATS-ISS_L1B_N-M7.2-V2-07

    Atmospheric Science Data Center

    2016-10-24

    CATS-ISS_L1B_N-M7.2-V2-07 The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive lidar that provides ... in the Earth's atmosphere. Project Title:  CATS Discipline:  Clouds Aerosols Version:  ...

  4. CATS-ISS_L1B_D-M7.2-V2-07

    Atmospheric Science Data Center

    2016-10-24

    CATS-ISS_L1B_D-M7.2-V2-07 The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive lidar that provides ... in the Earth's atmosphere. Project Title:  CATS Discipline:  Clouds Aerosols Version:  ...

  5. CATS-ISS_L1B_D-M7.2-V2-05

    Atmospheric Science Data Center

    2016-05-03

    CATS-ISS_L1B_D-M7.2-V2-05 The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive lidar that provides ... in the Earth's atmosphere. Project Title:  CATS Discipline:  Clouds Aerosols Version:  ...

  6. Characterization of a CTX-M-15 Producing Klebsiella Pneumoniae Outbreak Strain Assigned to a Novel Sequence Type (1427).

    PubMed

    Zhou, Kai; Lokate, Mariëtte; Deurenberg, Ruud H; Arends, Jan; Lo-Ten Foe, Jerome; Grundmann, Hajo; Rossen, John W A; Friedrich, Alexander W

    2015-01-01

    Extended-spectrum -lactamase producing Klebsiella pneumoniae have emerged as one of the major nosocomial pathogens. Between July and September 2012, a CTX-M-15 producing K. pneumoniae caused an outbreak in a university hospital in the Netherlands. The outbreak isolates were characterized and assigned to a novel sequence type (ST1427). An epidemiological link between affected patients was supported by patient contact tracing and whole-genome phylogenetic analysis. Intra-strain polymorphism was detected among multiple isolates obtained from different body sites of the index patient, which may relate to antibiotic treatment and/or host adaptation. Environmental contamination caused by the outbreak clone was found in the patient rooms even on medical equipment. The novel clone was not closely related to any known endemic/epidemic clone, but carried a set of a plasmid-borne resistance genes [bla CTX-M-15, bla TEM-1, bla OXA-1, aac(6')-Ib-cr, qnrB1, tetA(A), aac(3)-II]. Analysis of its virulence factors revealed a previously uncharacterized capsular biosynthesis region and two uncharacterized fimbriae gene clusters, and suggested that the new clone was not hypervirulent. To our knowledge, this is the first outbreak report of K. pneumoniae ST1427, and our study could be of help to understand the features of this newly emerging clone. PMID:26617589

  7. First Characterization of CTX-M-15-Producing Escherichia coli ST131 and ST405 Clones Causing Community-Onset Infections in South America▿

    PubMed Central

    Ruiz, Sory J.; Montealegre, Maria Camila; Ruiz-Garbajosa, Patricia; Correa, Adriana; Briceño, David F.; Martinez, Ernesto; Rosso, Fernando; Muñoz, Martin; Quinn, John P.; Cantón, Rafael; Villegas, Maria Virginia

    2011-01-01

    CTX-M-15-producing Escherichia coli has emerged worldwide as an important pathogen associated with community-onset infections, but in South America reports are scarce. We document the presence of CTX-M-15-producing E. coli of the international ST131 and ST405 clones in Colombia and present the first molecular characterization of these isolates in South America. PMID:21325548

  8. Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital.

    PubMed

    Melegh, Szilvia; Schneider, György; Horváth, Marianna; Jakab, Ferenc; Emődy, Levente; Tigyi, Zoltán

    2015-09-01

    We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period 2004-2008. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla(CTX-M), bla(TEM) and bla(SHV)) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofloxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identified, and were shown to harbour the following β-lactamase genes: six clones carried bla(CTX-M), four clones harboured bla(SHV-5) and one clone possessed both bla(CTX-M) and ESBL type bla(SHV). Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profile were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofloxacin, minor clone isolates displayed significantly lower MIC values for ciprofloxacin suggesting a role for fluoroquinolones in the dissemination of the major K. pneumoniae clones. This is the first description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary. PMID:26551567

  9. Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital.

    PubMed

    Melegh, Szilvia; Schneider, György; Horváth, Marianna; Jakab, Ferenc; Emődy, Levente; Tigyi, Zoltán

    2015-09-01

    We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period 2004-2008. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla(CTX-M), bla(TEM) and bla(SHV)) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofloxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identified, and were shown to harbour the following β-lactamase genes: six clones carried bla(CTX-M), four clones harboured bla(SHV-5) and one clone possessed both bla(CTX-M) and ESBL type bla(SHV). Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profile were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofloxacin, minor clone isolates displayed significantly lower MIC values for ciprofloxacin suggesting a role for fluoroquinolones in the dissemination of the major K. pneumoniae clones. This is the first description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary.

  10. Detection of chromosomal blaCTX-M-15 in Escherichia coli O25b-B2-ST131 isolates from the Kinki region of Japan.

    PubMed

    Hirai, Itaru; Fukui, Naoki; Taguchi, Masumi; Yamauchi, Kou; Nakamura, Tatsuya; Okano, Sho; Yamamoto, Yoshimasa

    2013-12-01

    Escherichia coli O25b-B2-ST131 isolates harbouring bla(CTX-M-15) are distributed worldwide. The bla(CTX-M-15) transposition unit has often been found on plasmids and the genetic contexts have been examined; however, less is known about the frequency and contexts of the bla(CTX-M-15) transposition unit on the chromosome. This study was performed to determine the chromosomal location of the bla(CTX-M-15) transposition unit and to analyse the molecular structure of the region surrounding the bla(CTX-M-15) transposition unit in E. coli O25b-B2-ST131 isolates. Twenty-two E. coli O25b-B2-ST131 strains harbouring bla(CTX-M-15) that had been isolated from university hospital patients and nursing home residents in the Kinki region of Japan were examined. Inverse PCR (iPCR) targeting bla(CTX-M-15) was performed to classify the molecular structure of the region surrounding the bla(CTX-M-15) transposition unit. The isolates were classified into nine types (types A-I) considering the iPCR results; type A was the most prevalent type (13/22 isolates). Sequences of the iPCR-amplified DNA fragments showed that the bla(CTX-M-15) transposition unit consisted of ISEcp1, bla(CTX-M-15) and orf477Δ. A homology search of the obtained sequences showed that the bla(CTX-M-15) transposition unit was inserted into different chromosomal regions in eight of the nine classified types. Although 21 of the 22 E. coli isolates possessed chromosomally located bla(CTX-M-15) transposition units, clonal spread was not evident on pulsed-field gel electrophoresis (PFGE) analysis. Taken together, these data indicate that certain E. coli O25b-B2-ST131 strains harbouring chromosomal bla(CTX-M-15) have emerged and spread in the Kinki region of Japan. PMID:24091130

  11. Predictability of Great Earthquakes: The 25 April 2015 M7.9 Gorkha (Nepal)

    NASA Astrophysics Data System (ADS)

    Kossobokov, V. G.

    2015-12-01

    Understanding of seismic process in terms of non-linear dynamics of a hierarchical system of blocks-and-faults and deterministic chaos, has already led to reproducible intermediate-term middle-range prediction of the great and significant earthquakes. The technique based on monitoring charcteristics of seismic static in an area proportional to source size of incipient earthquake is confirmed at the confidence level above 99% by statistics of Global Testing in forward application from 1992 to the present. The semi-annual predictions determined for the next half-year by the algorithm M8 aimed (i) at magnitude 8+ earthquakes in 262 circles of investigation, CI's, each of 667-km radius and (ii) at magnitude 7.5+ earthquakes in 180 CI's, each of 427-km radius are communicated each January and July to the Global Test Observers (about 150 today). The pre-fixed location of CI's cover all seismic regions where the M8 algorithm could run in its original version that requires annual rate of activity of 16 or more main shocks. According to predictions released in January 2015 for the first half of 2015, the 25 April 2015 Nepal MwGCMT = 7.9 earthquake falls outside the Test area for M7.5+, while its epicenter is within the accuracy limits of the alarm area for M8.0+ that spread along 1300 km of Himalayas. We note that (i) the earthquake confirms identification of areas prone to strong earthquakes in Himalayas by pattern recognition (Bhatia et al. 1992) and (ii) it would have been predicted by the modified version of the M8 algorithm aimed at M7.5+. The modified version is adjusted to a low level of earthquake detection, about 10 main shocks per year, and is tested successfully by Mojarab et al. (2015) in application to the recent earthquakes in Eastern Anatolia (23 October 2011, M7.3 Van earthquake) and Iranian Plateau (16 April 2013, M7.7 Saravan and the 24 September 2013, M7.7 Awaran earthquakes).

  12. A Plasmid Bearing the bla(CTX-M-15) Gene and Phage P1-Like Sequences from a Sequence Type 11 Klebsiella pneumoniae Isolate.

    PubMed

    Shin, Juyoun; Ko, Kwan Soo

    2015-10-01

    Plasmid pKP12226 was extracted and analyzed from a CTX-M-15-producing Klebsiella pneumoniae sequence type 11 (ST11) isolate collected in South Korea. The plasmid represents chimeric characteristics consisting of a pIP1206-like backbone and lysogenized phage P1-like sequences. It bears a resistance region that includes resistance genes to several antibiotics and is different from previously characterized plasmids from South Korea bearing blaCTX-M-15. It may have resulted from recombination between an Escherichia coli plasmid backbone, a blaCTX-M-15-bearing resistance region, and lysogenized phage P1-like sequences. PMID:26195513

  13. Tropospheric aerosol size distributions simulated by three online global aerosol models using the M7 microphysics module

    SciTech Connect

    Zhang, Kai; Wan, Hui; Wang, Bin; Zhang, Meigen; Feichter, J.; Liu, Xiaohong

    2010-07-14

    Tropospheric aerosol size distributions are simulated by three online global models that employ exactly the same modal approach but differ in many aspects such as model meteorology, natural aerosol emissions, sulfur chemistry, and the parameterization of deposition processes. The main purpose of this study is to identify where the largest inter-model discrepancies occur and what the main reasons are. The number concentrations of different aerosol size ranges are compared among the three models and against observations. Overall all the three models can capture the basic features of the observed aerosol number spatial distributions. The magnitude of the number concentration of each mode is consistent among the three models. Quantitative differences are also clearly detectable. For the soluble and insoluble coarse mode and accumulation mode, inter-model discrepancies mainly result from differences in the sea salt and dust emissions, as well as the different strengths of the convective transport in the meteorological models. For the nucleation mode and the soluble Aitken mode, the spread of the model results is largest in the tropics and in the middle and upper troposphere. Diagnostics and sensitivity experiments suggest that this large spread is closely related to the sulfur cycle in the models, which is strongly affected by the choice of sulfur chemistry scheme, its coupling with the convective transport and wet deposition calculation, and the related meteorological fields such as cloud cover, cloud water content, and precipitation. The aerosol size distributions simulated by the three models are compared to observations in the boundary layer. The characteristic shape and magnitude of the distribution functions are reasonably reproduced in typical conditions (i.e., clean, polluted and transition areas). Biases in the mode parameters over the remote oceans and the China adjacent seas are probably caused by the fixed mode variance in the mathematical formulations used in the modal approach in the three models, as well as some of the prescribed size distribution parameters of the natural and anthropogenic emissions.

  14. Establishing best practise in the application of expert review of mutagenicity under ICH M7.

    PubMed

    Barber, Chris; Amberg, Alexander; Custer, Laura; Dobo, Krista L; Glowienke, Susanne; Van Gompel, Jacky; Gutsell, Steve; Harvey, Jim; Honma, Masamitsu; Kenyon, Michelle O; Kruhlak, Naomi; Muster, Wolfgang; Stavitskaya, Lidiya; Teasdale, Andrew; Vessey, Jonathan; Wichard, Joerg

    2015-10-01

    The ICH M7 guidelines for the assessment and control of DNA reactive (mutagenic) impurities in pharmaceuticals allows for the consideration of in silico predictions in place of in vitro studies. This represents a significant advance in the acceptance of (Q)SAR models and has resulted from positive interactions between modellers, regulatory agencies and industry with a shared purpose of developing effective processes to minimise risk. This paper discusses key scientific principles that should be applied when evaluating in silico predictions with a focus on accuracy and scientific rigour that will support a consistent and practical route to regulatory submission.

  15. Characterization of a CTX-M-15 Producing Klebsiella Pneumoniae Outbreak Strain Assigned to a Novel Sequence Type (1427)

    PubMed Central

    Zhou, Kai; Lokate, Mariëtte; Deurenberg, Ruud H.; Arends, Jan; Lo-Ten Foe, Jerome; Grundmann, Hajo; Rossen, John W. A.; Friedrich, Alexander W.

    2015-01-01

    Extended-spectrum -lactamase producing Klebsiella pneumoniae have emerged as one of the major nosocomial pathogens. Between July and September 2012, a CTX-M-15 producing K. pneumoniae caused an outbreak in a university hospital in the Netherlands. The outbreak isolates were characterized and assigned to a novel sequence type (ST1427). An epidemiological link between affected patients was supported by patient contact tracing and whole-genome phylogenetic analysis. Intra-strain polymorphism was detected among multiple isolates obtained from different body sites of the index patient, which may relate to antibiotic treatment and/or host adaptation. Environmental contamination caused by the outbreak clone was found in the patient rooms even on medical equipment. The novel clone was not closely related to any known endemic/epidemic clone, but carried a set of a plasmid-borne resistance genes [blaCTX−M−15, blaTEM−1, blaOXA−1, aac(6′)-Ib-cr, qnrB1, tetA(A), aac(3)-II]. Analysis of its virulence factors revealed a previously uncharacterized capsular biosynthesis region and two uncharacterized fimbriae gene clusters, and suggested that the new clone was not hypervirulent. To our knowledge, this is the first outbreak report of K. pneumoniae ST1427, and our study could be of help to understand the features of this newly emerging clone. PMID:26617589

  16. Discovery of m7G-cap in eukaryotic mRNAs

    PubMed Central

    FURUICHI, Yasuhiro

    2015-01-01

    Terminal structure analysis of an insect cytoplasmic polyhedrosis virus (CPV) genome RNA in the early 1970s at the National Institute of Genetics in Japan yielded a 2′-O-methylated nucleotide in the 5′ end of double-stranded RNA genome. This finding prompted me to add S-adenosyl-L-methionine, a natural methylation donor, to the in vitro transcription reaction of viruses that contain RNA polymerase. This effort resulted in unprecedented mRNA synthesis that generates a unique blocked and methylated 5′ terminal structure (referred later to as “cap” or “m7G-cap”) in the transcription of silkworm CPV and human reovirus and vaccinia viruses that contain RNA polymerase in virus particles. Initial studies with viruses paved the way to discover the 5′-cap m7GpppNm structure present generally in cellular mRNAs of eukaryotes. I participated in those studies and was able to explain the pathway of cap synthesis and the significance of the 5′ cap (and capping) in gene expression processes, including transcription and protein synthesis. In this review article I concentrate on the description of these initial studies that eventually led us to a new paradigm of mRNA capping. PMID:26460318

  17. Earthquake nucleation by transient deformations caused by the M = 7.9 Denali, Alaska, earthquake

    USGS Publications Warehouse

    Gomberg, J.; Bodin, P.; Larson, K.; Dragert, H.

    2004-01-01

    The permanent and dynamic (transient) stress changes inferred to trigger earthquakes are usually orders of magnitude smaller than the stresses relaxed by the earthquakes themselves, implying that triggering occurs on critically stressed faults. Triggered seismicity rate increases may therefore be most likely to occur in areas where loading rates are highest and elevated pore pressures, perhaps facilitated by high-temperature fluids, reduce frictional stresses and promote failure. Here we show that the 2002 magnitude M = 7.9 Denali, Alaska, earthquake triggered wide-spread seismicity rate increases throughout British Columbia and into the western United States. Dynamic triggering by seismic waves should be enhanced in directions where rupture directivity focuses radiated energy, and we verify this using seismic and new high-sample GPS recordings of the Denali mainshock. These observations are comparable in scale only to the triggering caused by the 1992 M = 7.4 Landers, California, earthquake, and demonstrate that Landers triggering did not reflect some peculiarity of the region or the earthquake. However, the rate increases triggered by the Denali earthquake occurred in areas not obviously tectonically active, implying that even in areas of low ambient stressing rates, faults may still be critically stressed and that dynamic triggering may be ubiquitous and unpredictable.

  18. The 2008 M7.9 Wenchuan earthquake - Result of Local and Abnormal Mass Imbalances?

    NASA Astrophysics Data System (ADS)

    Klose, C. D.

    2008-12-01

    The May 12, 2008 M7.9 Wenchuan earthquake occurred along the Longmen Shan margin of the eastern Tibetan plateau in the Sichuan province of the People's Republic of China. A complex and NNW dipping reverse fault system including the Beichuan fault ruptured 250-300 km parallel to the Longmen Shan thrust belt. This region has been tectonically loaded for >10kyr. It has low deformation rates of less than 1.0±1.0 mm yr-1 resulting in no major seismic activity during the Quaternary period. Several geophysical observations suggest that this M7.9 earthquake was triggered by local and abnormal mass imbalances on the surface of the Earth's crust. These observations include (1) elastostatic response of the crust to the mass changes (2) slip distribution of the main rupture, and (3) aftershock distribution. Initially, approximately 2 years prior the nucleation of the mainshock, at least 320 million tonnes of water accumulated within the upper Min river valley. It enters the Chengdu plain of the Sichuan basin, a stable continental region (SCR). The water volume amplified the strain energy on the Earth's crust. Shear stresses increased by >1kPa on the Beichuan fault at the nucleation point in about 20km depth. Normal stresses decreased by <-4kPa and weakened the fault strength. Pore pressure increases might have additionally destabilized the fault locally due to pore pressure diffusion. This effect, however, might be minor in 20km depth, because of low lateral fracture connectivity and permeability between the area of water accumulation and the Beichuan fault. Overall, the stress alterations within a 120±70km2 large area resulted in the Beichuan fault coming closer to failure. Such an area ruptured would account for a M7.2±0.1 earthquake assuming only 10 MPa stress drop. Secondly, a reverse fault focal mechanism dominated, in particular, during the first 50 seconds of the main M7.9 rupture. The Beichuan fault slipped up to 7m upward peaking at shallow depth (<7km) (Nishimura

  19. Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG

    PubMed Central

    Gunawardana, Dilantha; Domashevskiy, Artem V; Gayler, Ken R; Goss, Dixie J

    2015-01-01

    A method has been developed for synthesising fluorescently labeled capped mRNA. The method incorporates a single fluorescent molecule as part of the 5′-mRNA or oligonucleotide cap site. The fluorescent molecule, Ant-m7GTP is specifically incorporated into the cap site to yield Ant-m7GpppG-capped mRNA or oligonucleotide. Efficient capping was observed with 60–100% of the RNA transcripts capped with the fluorescent molecule. The Ant-m7G derivative, which has been previously shown to interact with the eukaryotic cap binding protein eIF4E, is shown in this paper to be a substrate for the Vaccinia capping enzyme and the DCP2 decapping enzyme from Arabidopsis. Further, the Ant-m7GTP-capped RNA is readily translated. This Ant-m7GTP-capped RNA provides an important tool for monitoring capping reactions, translation, and biophysical studies. PMID:26779415

  20. Different IncI1 plasmids from Escherichia coli carry ISEcp1-blaCTX-M-15 associated with different Tn2-derived elements.

    PubMed

    Zong, Zhiyong; Ginn, Andrew N; Dobiasova, Hana; Iredell, Jonathan R; Partridge, Sally R

    2015-07-01

    The bla(CTX-M-15) gene, encoding the globally dominant CTX-M-15 extended-spectrum β-lactamase, has generally been found in a 2.971-kb ISEcp1-bla(CTX-M-15)-orf477Δ transposition unit, with ISEcp1 providing a promoter. In available IncF plasmid sequences from Escherichia coli, this transposition unit interrupts a truncated copy of transposon Tn2 that lies within larger multiresistance regions. In E. coli, bla(CTX-M-15) is also commonly associated with IncI1 plasmids and here three such plasmids from E. coli clinical isolates from western Sydney 2006-2007 have been sequenced. The plasmid backbones are organised similarly to those of other IncI1 plasmids, but have insertions and/or deletions and sequence differences. Each plasmid also has a different insertion carrying bla(CTX-M-15). pJIE113 (IncI1 sequence type ST31) is almost identical to plasmids isolated from the 2011 E. coli O104:H4 outbreak in Europe, where the typical bla(CTX-M-15) transposition unit interrupts a complete Tn2 inserted directly in the plasmid backbone. In the novel plasmid pJIE139 (ST88), ISEcp1-blaC(TX-M-15)-orf477Δ lies within a Tn2/3 hybrid transposon. Homologous recombination could explain movement of ISEcp1-bla(CTX-M-15)-orf477Δ between copies of Tn2 on IncF and IncI1 plasmids and generation of the Tn2/3 hybrid. pJIE174 (ST37) is almost identical to pESBL-12 from the Netherlands and in these plasmids bla(CTX-M-15) is flanked by two copies of IS26 that truncate the transposition unit within a larger region bounded by the ends of Tn2. bla(CTX-M-15) and the associated ISEcp1-derived promoter may be able to move from this structure by the actions of IS26, independently of both ISEcp1 and Tn2.

  1. Camera trap records of animal activity prior to a M=7 earthquake in Northern Peru

    NASA Astrophysics Data System (ADS)

    Grant, R.; Raulin, J.; Freund, F.

    2013-12-01

    Earthquake (EQ) preparation is associated with geophysical changes occurring over many scales. Some pre-earthquake (pre-EQ) processes affect the ionosphere, others leave their mark on biota. We report (i) on ionospheric anomalies recorded prior to the M=7 Contamana EQ [1] in North-Eastern Peru, 134 km deep, associated with the subduction of the Nazca plate underneath the Northern Andes, (ii) on changes in animal activity recorded in the Yanachaga National Park, about 320 km from the EQ epicentre, over a 30 day period leading up to the M=7 seismic event. Night-time Very Low Frequency (VLF) phase data were analyzed for the period 01 June to 31 Oct. 2011 using propagation paths passing close to the Yanachaga Park from the NAA emitter (USA) to receivers PIU in Piura and PLO in Lima (Peru). Ionospheric phase perturbations were observed starting 2 weeks before the EQ with periodicities from few tens of secs to few minutes. Animal activity data were obtained by evaluating the images of a cluster of 10 motion-triggered cameras of the Tropical Ecology Assessment and Monitoring Network www.teamnetwork.org. We analyzed 1359 photographic records for the pre-EQ period and 1491 photographic records for a control period with low seismicity. Animal activity started to noticeably decline 3 weeks before the EQ. Different animal species were found to react differently. The number of rodents declined to zero about one week before the EQ and so did the number of tapirs. Armadillos, a burrowing animal, were recorded in larger numbers. Though the armadillos were presumably also flushed out of their holes, they apparently did not hide like the rodents. We discuss the results in the context of recent advances in solid state physics, which provide plausible mechanisms for pre-EQ ionospheric anomalies and for changes in animal behavior. [1] Tavera, H. (2012), Report on the 24 Aug. 2011 M 7.0 Contamana, Peru, Intermediate Depth Earthquake Seismological Research Letters, 83, 1007-1013, doi: 10.1785/0220120005

  2. Surface Rupture and Slip Distribution Resulting from the 2013 M7.7 Balochistan, Pakistan Earthquake

    NASA Astrophysics Data System (ADS)

    Reitman, N. G.; Gold, R. D.; Briggs, R. W.; Barnhart, W. D.; Hayes, G. P.

    2014-12-01

    The 24 September 2013 M7.7 earthquake in Balochistan, Pakistan, produced a ~200 km long left-lateral strike-slip surface rupture along a portion of the Hoshab fault, a moderately dipping (45-75º) structure in the Makran accretionary prism. The rupture is remarkably continuous and crosses only two (0.7 and 1.5 km wide) step-overs along its arcuate path through southern Pakistan. Displacements are dominantly strike-slip, with a minor component of reverse motion. We remotely mapped the surface rupture at 1:5,000 scale and measured displacements using high resolution (0.5 m) pre- and post-event satellite imagery. We mapped 295 laterally faulted stream channels, terrace margins, and roads to quantify near-field displacement proximal (±10 m) to the rupture trace. The maximum near-field left-lateral offset is 15±2 m (average of ~7 m). Additionally, we used pre-event imagery to digitize 254 unique landforms in the "medium-field" (~100-200 m from the rupture) and then measured their displacements compared to the post-event imagery. At this scale, maximum left-lateral offset approaches 17 m (average of ~8.5 m). The width (extent of observed surface faulting) of the rupture zone varies from ~1 m to 3.7 km. Near- and medium-field offsets show similar slip distributions that are inversely correlated with the width of the fault zone at the surface (larger offsets correspond to narrow fault zones). The medium-field offset is usually greater than the near-field offset. The along-strike surface slip distribution is highly variable, similar to the slip distributions documented for the 2002 Denali M7.9 earthquake and 2001 Kunlun M7.8 earthquake, although the Pakistan offsets are larger in magnitude. The 2013 Pakistan earthquake ranks among the largest documented continental strike-slip displacements, possibly second only to the 18+ m surface displacements attributed to the 1855 Wairarapa M~8.1 earthquake.

  3. Static stress change from the 8 October, 2005 M = 7.6 Kashmir earthquake

    USGS Publications Warehouse

    Parsons, T.; Yeats, R.S.; Yagi, Y.; Hussain, A.

    2006-01-01

    We calculated static stress changes from the devastating M = 7.6 earthquake that shook Kashmir on 8 October, 2005. We mapped Coulomb stress change on target fault planes oriented by assuming a regional compressional stress regime with greatest principal stress directed orthogonally to the mainshock strike. We tested calculation sensitivity by varying assumed stress orientations, target-fault friction, and depth. Our results showed no impact on the active Salt Range thrust southwest of the rupture. Active faults north of the Main Boundary thrust near Peshawar fall in a calculated stress-decreased zone, as does the Raikot fault zone to the northeast. We calculated increased stress near the rupture where most aftershocks occurred. The greatest increase to seismic hazard is in the Indus-Kohistan seismic zone near the Indus River northwest of the rupture termination, and southeast of the rupture termination near the Kashmir basin.

  4. Examining the ages of M7-L8 dwarfs with the BOSS Ultracool Dwarf sample

    NASA Astrophysics Data System (ADS)

    Schmidt, Sarah J.; Hawley, Suzanne L.; West, Andrew A.; Bochanski, John J.

    2016-01-01

    We present the latest results from the BOSS Ultracool Dwarfs (BUD) sample of 12998 M7-L8 dwarfs, identified from a combination of photometry and spectroscopy from the Sloan Digital Sky Survey (SDSS). Using a cross-match of the BUD sample to the Two Micron All-Sky Survey (2MASS) and the Wide-field Infrared Survey Explorer (WISE) catalogs, we measure both colors and proper motions for the majority of the sample. The proper motions, combined with radial velocities from SDSS spectra and updated distance estimates based on i-Ks colors, yield three-dimensional velocities for 9121 ultracool dwarfs. We usethese velocities as statistical proxies for age to identify and test other potential age indicators, including Hα emission, atomic line strengths, molecular band depths, and broad-band colors.

  5. Building M7-0505 Treatment Tank (SWMU 039) Annual Performance Monitoring Report

    NASA Technical Reports Server (NTRS)

    2015-01-01

    This Annual Performance Monitoring Report presents a summary of Interim Measure (IM) activities and an evaluation of data collected during the third year (June 2014 to September 2015) of operation, maintenance, and monitoring (OM&M) conducted at the Building M7-505 (M505) Treatment Tank area, Kennedy Space Center (KSC), Florida ("the Site"). Under KSC's Resource Conservation and Recovery Act Corrective Action Program, the M505 Treatment Tank area was designated Solid Waste Management Unit 039. Arcadis U.S., Inc. (Arcadis) began IM activities on January 10, 2012, after completion of construction of an in situ air sparge (IAS) system to remediate volatile organic compounds (VOCs) in groundwater at concentrations exceeding applicable Florida Department of Environmental Protection (FDEP) Chapter 62-777, Florida Administrative Code, Natural Attenuation Default Concentrations (NADCs). This report presents a summary of the third year of OM&M activities conducted between June 2014 and September 2015.

  6. CCD photometry of globular cluster core structure. I - NGC 6388, NGC 6624, and M15: One flat core and two cusps

    NASA Technical Reports Server (NTRS)

    Lugger, Phyllis M.; Cohn, Haldan; Grindlay, Jonathan E.; Bailyn, Charles D.; Hertz, Paul

    1987-01-01

    The first results of a major survey of globular cluster core structure are reported. Surface brightness profiles for three clusters are presented: one with a normal flat core profile (NGC 6388), and two with central cusps (NGC 6624 and M15). The profiles are fitted with both seeing-convolved King models and seeing-convolved power laws. A King model provides a reasonable fit to the entire profile of NGC 6388, but there are no acceptable King profiles for NGC 6624 and M15. For these two clusters, a seeing-convolved power law provides a good fit to the inner part of the profile, while a King model gives a good fit to the outer part of the profile. The interpretation of the measured power-law slopes of the central surface brightness cusps of NGC 6624 and M15 are discussed in terms of models for core collapse in a multicomponent cluster.

  7. Earthquake Forecasts for Gorkha Immediately Following the 25th April, M=7.8 Mainshock

    NASA Astrophysics Data System (ADS)

    Segkou, M.; Parsons, T.

    2015-12-01

    The M-7.8 Gorkha (Nepal) earthquake on the 25th April, 2015 has shaken the central Himalayan front and immediately raised concerns for the severity of future triggered earthquakes. Here, we implement standard and innovative forecast models to predict the spatio-temporal distribution of triggered events. Key challenges addressed are: 1) the limited information on early aftershocks, 2) the low-productivity aftershock sequence in the near-source area, 3) the off-fault (>250 km) triggered events exemplified by the M=5.4 Xegar event, 3 hrs after the mainshock. We apply short-term empirical/statistical ETAS and physical forecast models, the latter based on the combination of rate/state friction law and Coulomb stresses. Within the physics-based model implementation we seek to evaluate the uncertainty related with the rupture style of triggered events by considering: 1) the geometry of active structures, 2) optimally oriented for failure faults and 3) all-potential faults described by the total stress field. The latter is represented by the full stress tensor before and after the mainshock and our analysis suggests that the preseismic stress magnitudes are still sufficient to cause earthquakes even after modification by the mainshock. The above remark reveals that there are no "stress shadows" affecting the spatial distribution of near-field aftershocks. It is also noted that the method allows for an a-priori determination of the rupture plan of the M=7.3 event, within the limit of uncertainty (20˚). The results show that: (1) ETAS models underestimate the number of observed events, since they heavily base their good performance in small magnitude earthquakes, not available in the first few weeks after the mainshock, (2) far field triggered events are captured only by physics-based forecasts, and (3) the total stress method improves the predictability of larger magnitude events. We conclude that frontier regions benefit from the implementation of physics-based models

  8. Evidence of shallow fault zone strengthening after the 1992 M7.5 landers, california, earthquake

    PubMed

    Li; Vidale; Aki; Xu; Burdette

    1998-01-01

    Repeated seismic surveys of the Landers, California, fault zone that ruptured in the magnitude (M) 7.5 earthquake of 1992 reveal an increase in seismic velocity with time. P, S, and fault zone trapped waves were excited by near-surface explosions in two locations in 1994 and 1996, and were recorded on two linear, three-component seismic arrays deployed across the Johnson Valley fault trace. The travel times of P and S waves for identical shot-receiver pairs decreased by 0.5 to 1.5 percent from 1994 to 1996, with the larger changes at stations located within the fault zone. These observations indicate that the shallow Johnson Valley fault is strengthening after the main shock, most likely because of closure of cracks that were opened by the 1992 earthquake. The increase in velocity is consistent with the prevalence of dry over wet cracks and with a reduction in the apparent crack density near the fault zone by approximately 1.0 percent from 1994 to 1996.

  9. Seismotectonic environment of occurring the February 3, 1996 Lijiang M=7.0 earthquake, Yunnan province

    NASA Astrophysics Data System (ADS)

    Han, Zhu-Jun; Guo, Shun-Min; Xiang, Hong-Fa; Zhang, Jia-Sheng; Ran, Yong-Kang

    2004-07-01

    Lijiang-Daju fault, the seismogenic fault of the 1996 Lijiang M=7.0 earthquake, can be divided into Lijiang-Yuhu segment in the south and Yuhu-Daju segment in the north. The two segments show clear difference in geological tectonics, but have the similar dynamic features. Both normal dip-slip and sinistral strike-slip coexist on the fault plane. This kind of movement started at the beginning of the Quaternary (2.4 2.5 Ma B.P.). As to the tectonic types, the detachment fault with low angle was developed in the Early Pleistocene and the normal fault with high angle only after the Mid-Pleistocene (0.8 Ma B.P.). Based on the horizontal displacements of gullies and the vertical variance of planation surfaces cross the Lijiang-Daju fault at east piedmont of Yulong-Haba range, the average horizontal and vertical slip rates are calculated. They are 0.84 mm/a and 0.70 mm/a since the Quaternary and 1.56 mm/a and 1.69 mm/a since the Mid-Pleistocene. The movements of the nearly N-S-trending Lijiang-Daju fault are controlled not only by the regional stress field, but also by the variant movement between the Yulong-Haba range and Lijiang basin. The two kinds of dynamic processes form the characteristics of seismotectonic environment of occurring the 1996 Lijiang earthquake.

  10. Evidence of shallow fault zone strengthening after the 1992 M7.5 Landers, California, earthquake

    USGS Publications Warehouse

    Li, Y.-G.; Vidale, J.E.; Aki, K.; Xu, Fei; Burdette, T.

    1998-01-01

    Repeated seismic surveys of the Landers, California, fault zone that ruptured in the magnitude (M) 7.5 earthquake of 1992 reveal an increase in seismic velocity with time. P, S, and fault zone trapped waves were excited by near-surface explosions in two locations in 1994 and 1996, and were recorded on two linear, three-component seismic arrays deployed across the Johnson Valley fault trace. The travel times of P and S waves for identical shot-receiver pairs decreased by 0.5 to 1.5 percent from 1994 to 1996, with the larger changes at stations located within the fault zone. These observations indicate that the shallow Johnson Valley fault is strengthening after the main shock, most likely because of closure of cracks that were opened by the 1992 earthquake. The increase in velocity is consistent with the prevalence of dry over wet cracks and with a reduction in the apparent crack density near the fault zone by approximately 1.0 percent from 1994 to 1996.

  11. Simulations of Orographic Mixed-Phase Clouds at Mountain Range Site using COSMO-ART-M7

    NASA Astrophysics Data System (ADS)

    Henneberg, Olga; Henneberger, Jan; Lohmann, Ulrike

    2015-04-01

    -moment microphysics scheme, show the occurrence of MPCs in accordance with the measurements with mass concentration of liquid and ice phase on the same order of magnitude as observed but the high ice number concentration observed at JFJ can not be captured by the simulations with the current setup. As the processes leading to such high ice concentration are not well understood yet it is not clear if they are considered by the model. The correlation between updraft velocities and occurrence of liquid water content found in the measurement results can also be seen in the first simulations with COSMO for some chosen days in February and April 2013 based on the measurements. Nevertheless the simulations also confirm that occurrence of MPC can not exclusively be explained by updraft velocities. Further simulations with modified aerosol concentrations and coupled to the ART-M7 module will explore influences of aerosols on clouds at JFJ.

  12. ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT, REMOVAL OF ARSENIC IN DRINKING WATER: WATTS PREMIER M-SERIES M-15,000 REVERSE OSMOSIS TREATMENT SYSTEM

    EPA Science Inventory

    Verification testing of the Watts Premier M-Series M-15,000 RO Treatment System was conducted over a 31-day period from April 26, 2004, through May 26, 2004. This test was conducted at the Coachella Valley Water District (CVWD) Well 7802 in Thermal, California. The source water...

  13. H.E.S.S. OBSERVATIONS OF THE GLOBULAR CLUSTERS NGC 6388 AND M15 AND SEARCH FOR A DARK MATTER SIGNAL

    SciTech Connect

    Abramowski, A.; Acero, F.; Akhperjanian, A. G.; Anton, G.; Balzer, A.; Brucker, J.; Barnacka, A.; Barres de Almeida, U.; Bazer-Bachi, A. R.; Borrel, V.; Becker, J.; Behera, B.; Boisson, C.; Bolmont, J.

    2011-07-01

    Observations of the globular clusters (GCs) NGC 6388 and M15 were carried out by the High Energy Stereoscopic System array of Cherenkov telescopes for a live time of 27.2 and 15.2 hr, respectively. No gamma-ray signal is found at the nominal target position of NGC 6388 and M15. In the primordial formation scenario, GCs are formed in a dark matter (DM) halo and DM could still be present in the baryon-dominated environment of GCs. This opens the possibility of observing a DM self-annihilation signal. The DM content of the GCs NGC 6388 and M15 is modeled taking into account the astrophysical processes that can be expected to influence the DM distribution during the evolution of the GC: the adiabatic contraction of DM by baryons, the adiabatic growth of a black hole in the DM halo, and the kinetic heating of DM by stars. Ninety-five percent confidence level exclusion limits on the DM particle velocity-weighted annihilation cross section are derived for these DM halos. In the TeV range, the limits on the velocity-weighted annihilation cross section are derived at the 10{sup -25} cm{sup 3} s{sup -1} level and a few 10{sup -24} cm{sup 3} s{sup -1} for NGC 6388 and M15, respectively.

  14. Detection and molecular characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-lactamases from bovine mastitis isolates in the United Kingdom.

    PubMed

    Timofte, Dorina; Maciuca, Iuliana E; Evans, Nicholas J; Williams, Helen; Wattret, Andrew; Fick, Jenny C; Williams, Nicola J

    2014-01-01

    Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried blaCTX-M-15 and blaTEM-1, while K. pneumoniae subsp. pneumoniae isolates carried blaSHV-12 and blaTEM-1. Conjugation experiments demonstrated that blaCTX-M-15 and blaTEM-1 were carried on a conjugative plasmid in E. coli, and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable in K. pneumoniae subsp. pneumoniae isolates. Moreover, in the E. coli isolates, an association of ISEcp1 and IS26 with blaCTX-M-15 was found where the IS26 element was inserted upstream of both ISEcp1 and the blaCTX-M promoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due to E. coli CTX-M-15 and also of bovine mastitis due to K. pneumoniae subsp. pneumoniae SHV-12 β-lactamases in the United Kingdom. We also describe the genetic environment of blaCTX-M-15 and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates.

  15. Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom

    PubMed Central

    Maciuca, Iuliana E.; Evans, Nicholas J.; Williams, Helen; Wattret, Andrew; Fick, Jenny C.; Williams, Nicola J.

    2014-01-01

    Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried blaCTX-M-15 and blaTEM-1, while K. pneumoniae subsp. pneumoniae isolates carried blaSHV-12 and blaTEM-1. Conjugation experiments demonstrated that blaCTX-M-15 and blaTEM-1 were carried on a conjugative plasmid in E. coli, and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable in K. pneumoniae subsp. pneumoniae isolates. Moreover, in the E. coli isolates, an association of ISEcp1 and IS26 with blaCTX-M-15 was found where the IS26 element was inserted upstream of both ISEcp1 and the blaCTX-M promoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due to E. coli CTX-M-15 and also of bovine mastitis due to K. pneumoniae subsp. pneumoniae SHV-12 β-lactamases in the United Kingdom. We also describe the genetic environment of blaCTX-M-15 and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates. PMID:24247146

  16. Genetic Environment of Plasmid Mediated CTX-M-15 Extended Spectrum Beta-Lactamases from Clinical and Food Borne Bacteria in North-Eastern India

    PubMed Central

    Upadhyay, Supriya; Hussain, Abbas; Mishra, Shweta; Maurya, Anand Prakash; Bhattacharjee, Amitabha; Joshi, Santa Ram

    2015-01-01

    Background The study investigated the presence of CTX-M-15 type extended spectrum beta-lactamases (ESBL), compared their genetic arrangements and plasmid types in gram negative isolates of hospital and food origin in north-east India. From September 2013 to April 2014, a total of 252 consecutive, non-duplicate clinical isolates and 88 gram negative food isolates were selected. Phenotypic and molecular characterization of ESBL genes was performed. Presence of integrons and gene cassettes were analyzed by integrase and 59 base-element PCR respectively. The molecular environments surrounding blaCTX-M and plasmid types were investigated by PCR and PCR-based replicon typing respectively. Transformation was carried out to assess plasmid transfer. Southern blotting was conducted to localize the blaCTX-M-15 genes. DNA fingerprinting was performed by ERIC-PCR. Results Prevalence of ESBL was found to be 40.8% (103/252) in clinical and 31.8% (28/88) in food-borne isolates. Molecular characterization revealed the presence of 56.3% (58/103) and 53.5% (15/28) blaCTX-M-15 in clinical and food isolates respectively. Strains of clinical and food origin were non-clonal. Replicon typing revealed that IncI1 and IncFII plasmid were carrying blaCTX-M-15 in clinical and food isolates and were horizontally transferable. The ISEcp1 element was associated with blaCTX-M-15 in both clinical and food isolates. Conclusions The simultaneous presence of resistance determinants in non-clonal isolates of two different groups thus suggests that the microbiota of common food products consumed may serve as a reservoir for some of the drug resistance genes prevalent in human pathogens. PMID:26361395

  17. Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane.

    PubMed

    Aparicio, JuanDaniel; Solá, María Zoleica Simón; Benimeli, Claudia Susana; Amoroso, María Julia; Polti, Marta Alejandra

    2015-06-01

    The aim of this work was to study the impact of environmental factors on the bioremediation of Cr(VI) and lindane contaminated soil, by an actinobacterium, Streptomyces sp. M7, in order to optimize the process. Soil samples were contaminated with 25 µg kg(-1) of lindane and 50 mg kg(-1) of Cr(VI) and inoculated with Streptomyces sp. M7. The lowest inoculum concentration which simultaneously produced highest removal of Cr(VI) and lindane was 1 g kg(-1). The influence of physical and chemical parameters was assessed using a full factorial design. The factors and levels tested were: Temperature: 25, 30, 35°C; Humidity: 10%, 20%, 30%; Initial Cr(VI) concentration: 20, 50, 80 mg kg(-1); Initial lindane concentration: 10, 25, 40 µg kg(-1). Streptomyces sp. M7 exhibited strong versatility, showing the ability to bioremediate co-contaminated soil samples at several physicochemical conditions. Streptomyces sp. M7 inoculum size was optimized. Also, it was fitted a model to study this process, and it was possible to predict the system performance, knowing the initial conditions. Moreover, optimum temperature and humidity conditions for the bioremediation of soil with different concentrations of Cr(VI) and lindane were determined. Lettuce seedlings were a suitable biomarker to evaluate the contaminants mixture toxicity. Streptomyces sp. M7 carried out a successful bioremediation, which was demonstrated through ecotoxicity test with Lactuca sativa. PMID:25749405

  18. Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane.

    PubMed

    Aparicio, JuanDaniel; Solá, María Zoleica Simón; Benimeli, Claudia Susana; Amoroso, María Julia; Polti, Marta Alejandra

    2015-06-01

    The aim of this work was to study the impact of environmental factors on the bioremediation of Cr(VI) and lindane contaminated soil, by an actinobacterium, Streptomyces sp. M7, in order to optimize the process. Soil samples were contaminated with 25 µg kg(-1) of lindane and 50 mg kg(-1) of Cr(VI) and inoculated with Streptomyces sp. M7. The lowest inoculum concentration which simultaneously produced highest removal of Cr(VI) and lindane was 1 g kg(-1). The influence of physical and chemical parameters was assessed using a full factorial design. The factors and levels tested were: Temperature: 25, 30, 35°C; Humidity: 10%, 20%, 30%; Initial Cr(VI) concentration: 20, 50, 80 mg kg(-1); Initial lindane concentration: 10, 25, 40 µg kg(-1). Streptomyces sp. M7 exhibited strong versatility, showing the ability to bioremediate co-contaminated soil samples at several physicochemical conditions. Streptomyces sp. M7 inoculum size was optimized. Also, it was fitted a model to study this process, and it was possible to predict the system performance, knowing the initial conditions. Moreover, optimum temperature and humidity conditions for the bioremediation of soil with different concentrations of Cr(VI) and lindane were determined. Lettuce seedlings were a suitable biomarker to evaluate the contaminants mixture toxicity. Streptomyces sp. M7 carried out a successful bioremediation, which was demonstrated through ecotoxicity test with Lactuca sativa.

  19. Macroscopic anomalies before the September 2010 M = 7.1 earthquake in Christchurch, New Zealand

    NASA Astrophysics Data System (ADS)

    Whitehead, N. E.; Ulusoy, Ü.

    2013-01-01

    Previous published work after the Kobe and İzmit earthquakes (1995 and 1999, respectively) demonstrated some reported meteorological and animal behaviour precursors were valid. Predictions were freshly tested for the Christchurch earthquake (M = 7.1, 4 September 2010). An internet survey with nearly 400 valid replies showed relative numbers of reports in precursor categories the day before the quake, were statistically significantly different from those in the preceding three days (excess meteorological events and animal behaviour). The day before the quake, there was also altered relative precursor class occurrence within 56 km compared with further away. Both these confirmed the earlier published work. Owners were woken up by unique pet behaviour 12 times as often in the hour before the quake compared with other hours immediately before (statistically highly significant). Lost and Found pet reports were double normal the week before, and 4.5 times normal both the day before the quake, and 9 days before. (Results were again statistically significant). Unique animal behaviour before the quake was often repeated before the numerous aftershocks. These pet owners claimed an approximate 80% prediction reliability. However, a preliminary telephone survey suggested that animals showing any precursor response are a minority. Some precursors seem real, but usefulness seemed mostly restricted to 7 cases where owners were in, or near, a place of safety through disruptive pet behaviour, and one in which owners were diverted by a pet from being struck by falling fixtures. For a later 22 February 2011 M = 6.3 quake no reports of escape through warning by pets were recorded, which raises serious questions whether such prediction is practically useful, because lives claimed saved are extremely low compared with fatalities. It is shown the lost-pet statistics dates, correspond to ionospheric anomalies recorded using the GPS satellite system and geomagnetic disturbance data, and

  20. GPS-Observed Displacements for the M7.7 October 27, 2012, Haida Gwaii Earthquake

    NASA Astrophysics Data System (ADS)

    Nykolaishen, L.; Dragert, H.; Wang, K.; Schmidt, M.; Lu, Y.; Schofield, B.

    2013-12-01

    On October 27, 2012, Canada's second largest (M7.7) instrumentally-recorded earthquake occurred off the west coast of southern Haida Gwaii. Unlike the 1949 M8.1 earthquake on the Queen Charlotte Fault, which involved characteristic strike-slip motion between the Pacific and North America plates, this earthquake involved low-angle thrust almost perpendicular to the coastal margin and generated large tsunami waves on the local coast. Here, the age of the underthrusting Pacific plate is only about 8 Ma, similar to that of the subducting Juan de Fuca plate along the Cascadia subduction zone, resulting in a very warm thermal environment. As a shallow rupture along a 'warm megathrust', the Haida Gwaii event can be viewed as a small-scale analogy of a great Cascadia earthquake. To refine regional source models, we investigate the associated co- and post-seismic displacements using a small, high precision GPS network installed in the weeks following the event. Co-seismic offsets at four re-occupied stations were estimated using historical data sets, with pre-earthquake positions determined by extrapolating linear velocities derived from earlier repeated campaigns. Preliminary results indicate movement at the coastal station closest (~30 km) to the epicentre of 1.2 m to the SSW, with 30 cm of subsidence. Operated by the Province of British Columbia, the only continuously recording station on Haida Gwaii, ~80 km from the epicentre, provided a more robust co-seismic estimate of 23 cm to the SSW. To date, estimates of cumulative post-seismic displacements from seven sites indicate up to 4 cm of motion, with a systematic along-strike variation in azimuthal directions between SSW and SE. The co-seismic results are consistent with a shallow-dipping thrust rupture underlying the Queen Charlotte Terrace which is located immediately seaward of the Queen Charlotte Fault; however, the observed horizontal and vertical displacements are not fully explained by rupture models based on

  1. Structural Distinction of Diacyl-, Alkylacyl, and Alk-1-Enylacyl Glycerophosphocholines as [M - 15]- Ions by Multiple-Stage Linear Ion-Trap Mass Spectrometry with Electrospray Ionization

    NASA Astrophysics Data System (ADS)

    Hsu, Fong-Fu; Lodhi, Irfan J.; Turk, John; Semenkovich, Clay F.

    2014-08-01

    We describe a linear ion-trap (LIT) multiple-stage (MSn) mass spectrometric approach towards differentiation of alkylacyl, alk-1-enylacyl- and diacyl-glycerophoscholines (PCs) as the [M - 15]- ions desorbed by electrospray ionization (ESI) in the negative-ion mode. The MS4 mass spectra of the [M - 15 - R2'CH = CO]- ions originated from the three PC subfamilies are readily distinguishable, resulting in unambiguous distinction of the lipid classes. This method is applied to two alkyl ether rich PC mixtures isolated from murine bone marrow neutrophils and kidney, respectively, to explore its utility in the characterization of complex PC mixture of biological origin, resulting in the realization of the detailed structures of the PC species, including various classes and many minor isobaric isomers.

  2. Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase–Producing Escherichia coli ST410 in the United Kingdom

    PubMed Central

    Maciuca, Iuliana Elena; Williams, Nicola J.; Wattret, Andrew; Schmidt, Vanessa

    2016-01-01

    We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-M to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15 and blaCTX-M-14, respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15–producing E. coli ST410. PMID:27314838

  3. The M7 October 21, 1868 Hayward Earthquake, Northern California-140 Years Later

    NASA Astrophysics Data System (ADS)

    Brocher, T. M.; Boatwright, J.; Lienkaemper, J. J.; Schwartz, D. P.; Garcia, S.

    2007-12-01

    October 21, 2008 marks the 140th anniversary of the M7 1868 Hayward earthquake. This large earthquake, which occurred slightly before 8 AM, caused extensive damage to San Francisco Bay Area and remains the nation's 12th most lethal earthquake. Property loss was extensive and about 30 people were killed. This earthquake culminated a decade-long series of earthquakes in the Bay Area which started with an M~6 earthquake in the southern Peninsula in 1856, followed by a series of four M5.8 to M6.1 sized earthquakes along the northern Calaveras fault, and ended with a M~6.5 earthquake in the Santa Cruz Mountains in 1865. Despite this flurry of quakes, the shaking from the 1868 earthquake was the strongest that the new towns and growing cities of the Bay Area had ever experienced. The effect on the brick buildings of the time was devastating: walls collapsed in San Francisco, Oakland, and San Jose, and buildings cracked as far away as Napa, Santa Rosa, and Hollister. The area that was strongly shaken (at Modified Mercalli Intensity VII or higher) encompassed about 2,300 km2. Aftershocks continued into November 1868. Surface cracking of the ground along the southern end of the Hayward Fault was traced from Warm Springs in Fremont northward 32 km to San Leandro. As Lawson (1908) reports, "the evidence to the northward of San Leandro is not very satisfactory. The country was then unsettled, and the information consisted of reports of cow- boys riding on the range". Analysis of historical triangulation data suggest that the fault moved as far north as Berkeley, and from these data the average slip along the fault is inferred to be about 1.9 ± 0.4 meters. The paleoseismic record from the southern end of the Hayward Fault provides evidence for 10 earthquakes before 1868. The average interval between these earthquakes is 170 ± 80 years, but the last five earthquakes have had an average interval of only 140 ± 50 years. The 1868 Hayward earthquake and more recent analogs such

  4. Dissemination of multidrug-resistant blaCTX-M-15/IncFIIk plasmids in Klebsiella pneumoniae isolates from hospital- and community-acquired human infections in Tunisia.

    PubMed

    Mansour, Wejdene; Grami, Raoudha; Ben Haj Khalifa, Anis; Dahmen, Safia; Châtre, Pierre; Haenni, Marisa; Aouni, Mahjoub; Madec, Jean-Yves

    2015-11-01

    This study investigated the molecular features of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae from hospital- and community-acquired (HA/CA) infections in the region of Mahdia, Tunisia. Among 336 K. pneumoniae isolates recovered from both clinical contexts between July 2009 and December 2011, 49 and 15 were ESBL producers and originated from clinical and community sources, respectively. All isolates produced the CTX-M-15 enzyme. As shown by Southern blot on S1 nuclease treatment followed by pulsed-field gel electrophoresis (PFGE) gels, the blaCTX-M-15 gene was carried on IncFII (n=4), IncFIIk (n=25), IncL/M (n=4), IncK (n=1), or untypeable (n=15) plasmids in HA isolates. In CA isolates, the blaCTX-M-15 gene was carried on IncFIIk (n=6), IncFII (n=1), IncHI1 (n=1), or untypeable (n=7) plasmids. In all, 23 and 11 PFGE types were found among the HA and CA isolates. Multilocus sequence typing on representative isolates shows diverse sequence types (STs), such as ST307, ST101, ST39, ST4, ST140, ST15, and ST307 in HA isolates and ST101, ST664, and ST323 in CA isolates. This study is the first comprehensive report of ESBL plasmids in K. pneumoniae from HA and CA infections in Tunisia.

  5. CATS-ISS_L2O_N-M7.2-V1-05_05kmLay

    Atmospheric Science Data Center

    2016-10-24

    CATS-ISS_L2O_N-M7.2-V1-05_05kmLay Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  6. CATS-ISS_L2O_D-M7.1-V1-05_05kmPro

    Atmospheric Science Data Center

    2016-10-25

    CATS-ISS_L2O_D-M7.1-V1-05_05kmPro Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  7. CATS-ISS_L2O_N-M7.2-V1-05_05kmPro

    Atmospheric Science Data Center

    2016-10-24

    CATS-ISS_L2O_N-M7.2-V1-05_05kmPro Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  8. An inter-laboratory comparison study of the ANSI/BIFMA standard test method M7.1 for furniture

    EPA Science Inventory

    Five laboratories using five different test chambers participated in the study to quantify within- and between-laboratory variability in the measurement of emissions of volatile organic compounds (VOCs) from new commercial furniture test items following ANSI/BIFMA M7.1. Test item...

  9. CATS-ISS_L2O_N-M7.2-V1-03_05kmPro

    Atmospheric Science Data Center

    2016-04-27

    CATS-ISS_L2O_N-M7.2-V1-03_05kmPro Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  10. CATS-ISS_L2O_N-M7.2-V1-04_05kmPro

    Atmospheric Science Data Center

    2016-06-30

    CATS-ISS_L2O_N-M7.2-V1-04_05kmPro Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  11. CATS-ISS_L2O_D-M7.2-V1-04_05kmLay

    Atmospheric Science Data Center

    2016-06-30

    CATS-ISS_L2O_D-M7.2-V1-04_05kmLay Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  12. CATS-ISS_L2O_N-M7.2-V1-03_05kmLay

    Atmospheric Science Data Center

    2016-04-27

    CATS-ISS_L2O_N-M7.2-V1-03_05kmLay Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  13. CATS-ISS_L2O_D-M7.2-V1-03_05kmLay

    Atmospheric Science Data Center

    2016-04-27

    CATS-ISS_L2O_D-M7.2-V1-03_05kmLay The Cloud-Aerosol Transport System (CATS) is a three wavelength, polarization-sensitive lidar that provides ... in the Earth's atmosphere. Project Title:  CATS Discipline:  Clouds Aerosols Version:  ...

  14. CATS-ISS_L2O_N-M7.2-V1-04_05kmLay

    Atmospheric Science Data Center

    2016-06-30

    CATS-ISS_L2O_N-M7.2-V1-04_05kmLay Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  15. CATS-ISS_L2O_D-M7.2-V1-04_05kmPro

    Atmospheric Science Data Center

    2016-06-30

    CATS-ISS_L2O_D-M7.2-V1-04_05kmPro Project Title:  CATS Discipline:  Clouds Aerosols Version:  ... and Order:  ASDC Order Tool Product Browse Tool:  CATS Order Tool OPeNDAP Access:  OPeNDAP Parameters:  ...

  16. Rupture process of the 2005 M7.2 Miyagi-Oki, NE Japan, earthquake inferred from coseismic slip and aftershock distributions, and its comparison with the 1978 M7.4 Miyagi-Oki earthquake

    NASA Astrophysics Data System (ADS)

    Okada, T.; Yaginuma, T.; Umino, N.; Kono, T.; Matsuzawa, T.; Kita, S.; Hasegawa, A.

    2005-12-01

    A large (M7.2) interplate earthquake occurred on 16 August 2005 in the Miyagi-Oki region, NE Japan. In this area, large interplate earthquakes with magnitude of ~M7.5 occurred repeatedly at a recurrence interval of ~37 yrs. Base on this recurrence of large earthquakes, the Headquarters of Earthquake Research Promotion of the Japanese government released the information of high probability (~50 percents within 10 years from now) of the impending earthquake to the public. To compare the aftershock distribution of the 2005 Miyagi-Oki earthquake with that of the previous (1978) Miyagi-Oki earthquake (M7.4), we relocated hypocenters of the aftershocks using double-difference method (Waldhauser and Ellsworth, 2000). Spatial extent of the aftershock area of the 2005 event is 80km and 40km in dip and strike directions, respectively. High aftershock activity was observed in the central part of the aftershock area, which is located to north-west of the mainshock hypocenter. The aftershock area of the 2005 event is overlapped with the southern/south-eastern part of that of the 1978 event. Locations of the aftershock clusters of the 2005 event correspond with those of the 1978 event and those of the background seismicity. We estimated coseismic slip distribution of the 2005 event by seismic waveform inversion by a method of Yagi et al. (2004). Obtained coseismic slipped area of the 2005 event extended mainly to the dip (northwest) direction, which is almost correspond with the area with high aftershock activity, and also partly overlapped with that of the 1978 event (Yamanaka & Kikuchi, 2004). These observations suggest that the spatial seismicity pattern on the plate boundary in subduction zone persists in time and the 2005 event have ruptured one of the asperities which caused the 1978 M7.4 event.

  17. Crystallization and preliminary crystallographic analysis of tRNA (m(7)G46) methyltransferase from Escherichia coli.

    PubMed

    Liu, Qi; Gao, Yang; Yang, Weili; Zhou, Huihao; Gao, Yongxiang; Zhang, Xiao; Teng, Maikun; Niu, Liwen

    2008-08-01

    Transfer RNA (tRNA) (m(7)G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-L-methionine (SAM) as the methyl-group donor to catalyze the formation of N(7)-methylguanosine (m(7)G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His(6) tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P2(1).

  18. Crystallization and preliminary crystallographic analysis of tRNA (m7G46) methyltransferase from Escherichia coli

    PubMed Central

    Liu, Qi; Gao, Yang; Yang, Weili; Zhou, Huihao; Gao, Yongxiang; Zhang, Xiao; Teng, Maikun; Niu, Liwen

    2008-01-01

    Transfer RNA (tRNA) (m7G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-l-methionine (SAM) as the methyl-group donor to catalyze the formation of N 7-­methylguanosine (m7G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His6 tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P21. PMID:18678947

  19. Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m(7)GpppG.

    PubMed

    Domashevskiy, Artem V; Rodriguez, David J; Gunawardana, Dilantha; Goss, Dixie J

    2016-01-01

    Fluorescent mRNA molecules offer a wide range of applications for studying capping/decapping reactions, translation, and other biophysical studies. Furthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce anthranioyl-m(7)GpppG-capped RNA. PMID:27236792

  20. Evidence of α-, β- and γ-HCH mixture aerobic degradation by the native actinobacteria Streptomyces sp. M7.

    PubMed

    Sineli, P E; Tortella, G; Dávila Costa, J S; Benimeli, C S; Cuozzo, S A

    2016-05-01

    The organochlorine insecticide γ-hexachlorocyclohexane (γ-HCH, lindane) and its non-insecticidal α- and β-isomers continue to pose serious environmental and health concerns, although their use has been restricted or completely banned for decades. In this study we report the first evidence of the growth ability of a Streptomyces strain in a mineral salt medium containing high doses of α- and β-HCH (16.6 mg l(-1)) as a carbon source. Degradation of HCH isomers by Streptomyces sp. M7 was investigated after 1, 4, and 7 days of incubation, determining chloride ion release, and residues in the supernatants by GC with µECD detection. The results show that both the α- and β-HCH isomers were effectively metabolized by Streptomyces sp. M7, with 80 and 78 % degradation respectively, after 7 days of incubation. Moreover, pentachlorocyclohexenes and tetrachlorocyclohexenes were detected as metabolites. In addition, the formation of possible persistent compounds such as chlorobenzenes and chlorophenols were studied by GC-MS, while no phenolic compounds were detected. In conclusion, we have demonstrated for the first time that Streptomyces sp. M7 can degrade α- and β-isomers individually or combined with γ-HCH and could be considered as a potential agent for bioremediation of environments contaminated by organochlorine isomers. PMID:27038951

  1. Evidence of α-, β- and γ-HCH mixture aerobic degradation by the native actinobacteria Streptomyces sp. M7.

    PubMed

    Sineli, P E; Tortella, G; Dávila Costa, J S; Benimeli, C S; Cuozzo, S A

    2016-05-01

    The organochlorine insecticide γ-hexachlorocyclohexane (γ-HCH, lindane) and its non-insecticidal α- and β-isomers continue to pose serious environmental and health concerns, although their use has been restricted or completely banned for decades. In this study we report the first evidence of the growth ability of a Streptomyces strain in a mineral salt medium containing high doses of α- and β-HCH (16.6 mg l(-1)) as a carbon source. Degradation of HCH isomers by Streptomyces sp. M7 was investigated after 1, 4, and 7 days of incubation, determining chloride ion release, and residues in the supernatants by GC with µECD detection. The results show that both the α- and β-HCH isomers were effectively metabolized by Streptomyces sp. M7, with 80 and 78 % degradation respectively, after 7 days of incubation. Moreover, pentachlorocyclohexenes and tetrachlorocyclohexenes were detected as metabolites. In addition, the formation of possible persistent compounds such as chlorobenzenes and chlorophenols were studied by GC-MS, while no phenolic compounds were detected. In conclusion, we have demonstrated for the first time that Streptomyces sp. M7 can degrade α- and β-isomers individually or combined with γ-HCH and could be considered as a potential agent for bioremediation of environments contaminated by organochlorine isomers.

  2. 7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity.

    PubMed

    Wypijewska, Anna; Bojarska, Elzbieta; Lukaszewicz, Maciej; Stepinski, Janusz; Jemielity, Jacek; Davis, Richard E; Darzynkiewicz, Edward

    2012-10-01

    Decapping scavenger (DcpS) enzymes catalyze the cleavage of a residual cap structure following 3' → 5' mRNA decay. Some previous studies suggested that both m(7)GpppG and m(7)GDP were substrates for DcpS hydrolysis. Herein, we show that mononucleoside diphosphates, m(7)GDP (7-methylguanosine diphosphate) and m(3)(2,2,7)GDP (2,2,7-trimethylguanosine diphosphate), resulting from mRNA decapping by the Dcp1/2 complex in the 5' → 3' mRNA decay, are not degraded by recombinant DcpS proteins (human, nematode, and yeast). Furthermore, whereas mononucleoside diphosphates (m(7)GDP and m(3)(2,2,7)GDP) are not hydrolyzed by DcpS, mononucleoside triphosphates (m(7)GTP and m(3)(2,2,7)GTP) are, demonstrating the importance of a triphosphate chain for DcpS hydrolytic activity. m(7)GTP and m(3)(2,2,7)GTP are cleaved at a slower rate than their corresponding dinucleotides (m(7)GpppG and m(3)(2,2,7)GpppG, respectively), indicating an involvement of the second nucleoside for efficient DcpS-mediated digestion. Although DcpS enzymes cannot hydrolyze m(7)GDP, they have a high binding affinity for m(7)GDP and m(7)GDP potently inhibits DcpS hydrolysis of m(7)GpppG, suggesting that m(7)GDP may function as an efficient DcpS inhibitor. Our data have important implications for the regulatory role of m(7)GDP in mRNA metabolic pathways due to its possible interactions with different cap-binding proteins, such as DcpS or eIF4E.

  3. Raman spectroscopic analysis of the clonal and horizontal spread of CTX-M-15-producing Klebsiella pneumoniae in a neonatal intensive care unit.

    PubMed

    Guyot, K; Biran, V; Doit, C; Moissenet, D; Guillard, T; Brasme, L; Courroux, C; Maquelin, K; van Leeuwen, W; Vuthien, H; Aujard, Y; De Champs, C; Bingen, E

    2012-10-01

    Nosocomial outbreaks of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae are an increasing concern in neonatal intensive care units (NICUs). We describe an outbreak of ESBL-producing K. pneumoniae that lasted 5 months and affected 23 neonates in our NICU. Proton pump inhibitor and extended-spectrum cephalosporin exposure were significantly associated with the risk of ESBL-producing K. pneumoniae colonisation and/or infection. Thirty isolates recovered from clinical, screening and environmental samples in the NICU were studied by means of Raman spectroscopy, pulsed-field gel electrophoresis and repetitive extragenic palindromic polymerase chain reaction (rep-PCR). The Raman clustering was in good agreement with the results of the other two molecular methods. Fourteen isolates belonged to the Raman clone 1 and 16 to the Raman clone 3. Molecular analysis showed that all the strains expressed SHV-1 chromosomal resistance, plasmid-encoded TEM-1 and CTX-M-15 β-lactamases. Incompatibility groups of plasmid content identified by PCR-based replicon typing indicated that resistance dissemination was due to the clonal spread of K. pneumoniae and horizontal CTX-M-15 gene transfer between the two clones.

  4. blaCTX-M-1/9/1 Hybrid Genes May Have Been Generated from blaCTX-M-15 on an IncI2 Plasmid

    PubMed Central

    Liu, Lanping; He, Dandan; Lv, Luchao; Liu, Wuling; Chen, Xiaojie; Zeng, Zhenling

    2015-01-01

    Three hybrid CTX-M β-lactamases, CTX-M-64, CTX-M-123, and CTX-M-132, with N and C termini matching CTX-M-1 group enzymes and centers matching CTX-M-9 group enzymes, have been identified. The hybrid gene sequences suggested recombination between blaCTX-M-15 and blaCTX-M-14, the two most common blaCTX-M variants worldwide. However, blaCTX-M-64 and blaCTX-M-123 are found in an ISEcp1-blaCTX-M transposition unit with a 45-bp “spacer,” rather than the 48 bp usually associated with blaCTX-M-15, and 112 bp of IncA/C plasmid backbone. This is closer to the context of blaCTX-M-55, which has one nucleotide difference from blaCTX-M-15, on IncI2 plasmid pHN1122-1. Here, we characterized an IncI2 plasmid carrying blaCTX-M-15 with a 45-bp spacer (pHNY2-1) by complete sequencing and also sequenced IncI2 plasmids carrying blaCTX-M-64 (pHNAH46-1) or blaCTX-M-132 (pHNLDH19) and an IncI1 plasmid carrying blaCTX-M-123 (pHNAH4-1). pHNY2-1 has the same ISEcp1-blaCTX-M-IncA/C insertion as pHN1122-1, pHNAH46-1, and pHNLDH19, and all four plasmid backbones are almost identical. pHNAH4-1 (IncI1 sequence type 108 [ST108]) carries a transposition unit that includes a 2,720-bp fragment of the IncI2 backbone, suggesting ISEcp1-mediated transfer of blaCTX-M-IncA/C-IncI2 to an IncI1 plasmid. All three hybrid blaCTX-M genes may have resulted from recombination between blaCTX-M-14 and blaCTX-M-15 with a 45-bp spacer on an IncI2 plasmid. Five additional Escherichia coli isolates of different sequence types from different provinces, farms, and/or animals had blaCTX-M-64 on a pHNAH46-1-like IncI2 plasmid and 9 had blaCTX-M-123 on a pHNAH4-1-like IncI1 ST108 plasmid. Thus, epidemic IncI plasmids may be responsible for the spread of blaCTX-M-64 and blaCTX-M-123 between different animals and different locations in China. PMID:25987615

  5. Molecular Epidemiological Analysis of Escherichia coli Sequence Type ST131 (O25:H4) and blaCTX-M-15 among Extended-Spectrum-β-Lactamase-Producing E. coli from the United States, 2000 to 2009

    PubMed Central

    Urban, Carl; Weissman, Scott J.; Jorgensen, James H.; Lewis, James S.; Hansen, Glen; Edelstein, Paul H.; Robicsek, Ari; Cleary, Timothy; Adachi, Javier; Paterson, David; Quinn, John; Hanson, Nancy D.; Johnston, Brian D.; Clabots, Connie; Kuskowski, Michael A.

    2012-01-01

    Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene blaCTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and blaCTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) blaCTX-M-15 negative non-ST131, (ii) blaCTX-M-15 positive non-ST131, (iii) blaCTX-M-15 negative ST131, or (iv) blaCTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited blaCTX-M-15, whereas 165 (47%) were ST131. ST131 accounted for 56% of blaCTX-M-15-positive- versus 35% of blaCTX-M-15-negative isolates (P < 0.001). Whereas ST131 accounted for 94% of the 175 total group B2 isolates, non-ST131 isolates were phylogenetically distributed by blaCTX-M-15 status, with groups A (blaCTX-M-15-positive isolates) and D (blaCTX-M-15-negative isolates) predominating. Both blaCTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non-ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. blaCTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and blaCTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health

  6. Coseismic Slip Model of the M 7.8 2015 Nepal Earthquake and its M 7.2 Aftershock from Joint Inversion of InSAR and GPS Data

    NASA Astrophysics Data System (ADS)

    Cheloni, D.; Tolomei, C.; Bignami, C.; D'Agostino, N.; Atzori, S.

    2015-12-01

    We derived a coseismic slip model for the M 7.8 2015 Nepal earthquake on the basis of radar line-of-sight displacements retrieved from RADARSAT, ALOS and SENTINEL interferograms and GPS data. We use the surface trace of the Main Himalayan Thrust (MHT) and moment tensor solution to approximate the earthquake rupture plane with a planar thrust fault having a strike of ~295° and a dip of ~10°. Our rupture model suggests that the slip area is about 120 x 50 km, with the most of the moment release limited to a depth between 10-20 km. Therefore the earthquake did not reach the surface suggesting a marked shallow slip deficit in the slip depth distribution. The earthquake released a seismic moment of 7.88E+20 Nm, corresponding to a Mw 7.88. Adopting the same fault geometry we also estimated the slip distribution related to the M 7.2 aftershock that occurred near the eastern end of the main coseismic asperity. The aftershock rupture model shows a more compact slip area of about 30 x 30 km, releasing a moment of 5.49E+19 Nm and corresponding to a Mw 7.13. We calculated the static stress changes on the assumed fault plane due to the mainshock. The result suggests loading of the fault around the main coseismic patch where indeed most of the aftershocks, comprising the M 7.2 event, were primarily distributed. Future researches will be focused on the up-dip locked portions of the MHT that did not break during the 25 April 2015 Nepal earthquake and the monitoring of the post-seismic phase by means of InSAR and GPS data.

  7. High Prevalence of Escherichia coli-Producing CTX-M-15 Extended-Spectrum Beta-Lactamases in Poultry and Human Clinical Isolates in Romania.

    PubMed

    Maciuca, Iuliana E; Williams, Nicola J; Tuchilus, Cristina; Dorneanu, Olivia; Guguianu, Eleonora; Carp-Carare, Catalin; Rimbu, Cristina; Timofte, Dorina

    2015-12-01

    Use of antibiotics in food animals may contribute to development and spread of resistant organisms, particularly so in some countries. The aim of this study was two-fold; first, to establish the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in chicken production in a region within Romania. Second, to study the relatedness of ESBL-producing E. coli isolates recovered from broilers, abattoir workers where the chickens were slaughtered and from the human clinical specimens from two regional hospitals. The results indicated a very high (69%) rate of carriage of ESBL and AmpC-producing E. coli in chickens with 36% CTX-M producers. Sequencing showed that chickens in Romania have the highest worldwide prevalence (53%) of blaCTX-M-15 reported in poultry E. coli isolates. The majority (53%) of the extended-spectrum cephalosporin-resistant E. coli carried plasmid-mediated blaampC genes, mostly blaCMY-2 type, one of the highest prevalences reported in Europe. The predominant CTX-M type found in the human clinical E. coli isolates was blaCTX-M-15 and most isolates coharbored blaOXA-1, blaTEM, and aac(6')-ib-cr. The majority (60%) of the human clinical isolates belonged to the pandemic virulent clone B2-ST131. The clonal relationship between broiler and the human CTX-M-producing E. coli isolates was assessed by macrorestriction pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), which indicated strain diversity with no common STs found between human and poultry isolates. Moreover, IncI1 was the most prevalent replicon found in broiler ESBL-producing E. coli isolates and also in transconjugants, indicating that plasmids and not clonal spread may play a role in the transfer of blaCTX-M genes. This study identifies a high prevalence of ESBL-producing E. coli from broiler chickens in Romania with a high occurrence incidence of blaCTX-M-15, which reflects the main ESBL type found in human E. coli infections in this

  8. First report of a Klebsiella pneumoniae ST466 strain causing neonatal sepsis harbouring the blaCTX-M-15 gene in Rabat, Morocco.

    PubMed

    Ballén, Victoria; Sáez, Emma; Benmessaoud, Rachid; Houssain, Tligui; Alami, Hassan; Barkat, Amina; Kabiri, Meryem; Moraleda, Cinta; Bezad, Rachid; Vila, Jordi; Bosch, Jordi; Bassat, Quique; Soto, Sara M

    2015-01-01

    Klebsiella pneumoniae is one of the Gram-negative bacilli most commonly found in urine of pregnant women and causing neonatal sepsis. The aim of this study was to analyse in terms of epidemiology and antimicrobial resistance of 23 K. pneumoniae isolates collected from vaginal swabs or urine of pregnant women, from pharyngeal and ear swabs of apparently healthy newborns and from peripheral cultures and hemocultures of newborns with suspected invasive neonatal infection in Rabat, Morocco. The prevalence of K. pneumoniae was 0.6 and 0.9% among pregnant women and neonates, respectively. These strains showed lower antimicrobial resistance levels regarding the developed countries. Thus, only one strain from a neonate presented an ESBL. This is the first report of a K. pneumoniae strain causing neonatal sepsis harbouring the blaCTX-M-15 gene in an IncFII plasmid and belonging to ST466 in this area.

  9. Outbreak caused by an ertapenem-resistant, CTX-M-15-producing Klebsiella pneumoniae sequence type 101 clone carrying an OmpK36 porin variant.

    PubMed

    Poulou, Aggeliki; Voulgari, Evangelia; Vrioni, Georgia; Koumaki, Vasiliki; Xidopoulos, Grigorios; Chatzipantazi, Vasiliki; Markou, Fani; Tsakris, Athanassios

    2013-10-01

    Although numerous studies have documented outbreaks of carbapenem-resistant Klebsiella pneumoniae (CRKP) possessing various carbapenemases, reports on outbreaks due to CRKP possessing extended-spectrum β-lactamases (ESBLs) and/or AmpCs with porin lesions have been limited. Here, we describe an outbreak caused by an ertapenem-resistant, CTX-M-15-producing clonal K. pneumoniae strain expressing an OmpK36 porin variant. From May 2012 to November 2012, 37 ertapenem-resistant K. pneumoniae isolates phenotypically negative for carbapenemase production were recovered from 19 patients hospitalized in the intensive care unit of a Greek hospital. The isolates were either susceptible or intermediate to other carbapenems and resistant to all remaining β-lactams but cefotetan. Phenotypic and molecular analysis revealed the presence in all isolates of the blaCTX-M-15 gene on a conjugative 100-kb plasmid, disruption in the expression of the ompK35 gene, and the production of an Ompk36 porin variant. The index case was a patient admitted from another hospital. Active surveillance upon admission and on a weekly basis was immediately initiated; environmental samples were also periodically tested. Molecular typing showed that all clinical isolates as well as two ertapenem-resistant environmental K. pneumoniae isolates belonged to the same clonal type and were assigned to multilocus sequence typing (MLST) sequence type 101 (ST101). As all colonized/infected patients were hospitalized during overlapping periods, cross-infection was considered the main route for the dissemination of the outbreak strain. Despite reinforcement of infection control measures and active surveillance, the outbreak lasted approximately 7 months. Identification of hidden carriers upon admission and by screening on a weekly basis was found valuable for early recognition and subsequent successful management of the outbreak.

  10. Outbreak Caused by an Ertapenem-Resistant, CTX-M-15-Producing Klebsiella pneumoniae Sequence Type 101 Clone Carrying an OmpK36 Porin Variant

    PubMed Central

    Poulou, Aggeliki; Voulgari, Evangelia; Vrioni, Georgia; Koumaki, Vasiliki; Xidopoulos, Grigorios; Chatzipantazi, Vasiliki; Markou, Fani

    2013-01-01

    Although numerous studies have documented outbreaks of carbapenem-resistant Klebsiella pneumoniae (CRKP) possessing various carbapenemases, reports on outbreaks due to CRKP possessing extended-spectrum β-lactamases (ESBLs) and/or AmpCs with porin lesions have been limited. Here, we describe an outbreak caused by an ertapenem-resistant, CTX-M-15-producing clonal K. pneumoniae strain expressing an OmpK36 porin variant. From May 2012 to November 2012, 37 ertapenem-resistant K. pneumoniae isolates phenotypically negative for carbapenemase production were recovered from 19 patients hospitalized in the intensive care unit of a Greek hospital. The isolates were either susceptible or intermediate to other carbapenems and resistant to all remaining β-lactams but cefotetan. Phenotypic and molecular analysis revealed the presence in all isolates of the blaCTX-M-15 gene on a conjugative 100-kb plasmid, disruption in the expression of the ompK35 gene, and the production of an Ompk36 porin variant. The index case was a patient admitted from another hospital. Active surveillance upon admission and on a weekly basis was immediately initiated; environmental samples were also periodically tested. Molecular typing showed that all clinical isolates as well as two ertapenem-resistant environmental K. pneumoniae isolates belonged to the same clonal type and were assigned to multilocus sequence typing (MLST) sequence type 101 (ST101). As all colonized/infected patients were hospitalized during overlapping periods, cross-infection was considered the main route for the dissemination of the outbreak strain. Despite reinforcement of infection control measures and active surveillance, the outbreak lasted approximately 7 months. Identification of hidden carriers upon admission and by screening on a weekly basis was found valuable for early recognition and subsequent successful management of the outbreak. PMID:23850951

  11. High-resolution imaging and crowded-field photometry of the stellar populations in the cores of the Globular Clusters M15 and M4

    NASA Astrophysics Data System (ADS)

    Butler, R. F.

    1999-02-01

    This thesis presents work performed at the Department of Physics, University College Galway from 1992 to 1997. It is concerned with ground- and space-based high-resolution optical imaging of globular cluster cores, and the subsequent application of image-restoration and crowded-field photometry techniques; thus we may gain an improved understanding of the nature of their stellar populations, by either monitoring their temporal behaviour over moderate periods for the first time, or by obtaining a more precise "static" picture than was hitherto possible. These goals can be achieved by the development of innovative instrumentation and data analysis techniques. The particularly unique aspect of this work is that it deals with the first application of two-dimensional photon-counting detectors (2D-PCDs) and post-exposure image sharpening (PEIS) for crowded-field photometry. The thesis starts by introducing some basic concepts and characteristics of globular clusters and the diverse stellar species which they contain, in particular those predicted to have formed as a result of dynamical processes in the cluster cores, and those which exhibit variability in emission over time. It then reviews the fields of high-resolution imaging through the turbulent atmosphere & image deconvolution, optical stellar photometry, and Hubble Space Telescope observing and data reduction, each concluded with a description of the systems used in the work reported here (for the HST chapter this involves photometry of WFPC2 (Wide Field & Planetary Camera 2) observations of M15 (NGC 7078) released into the archives in 1995). The core of the thesis begins with a review of the observations to date of the objects with which this thesis is chiefly concerned, M15 and M4 (NGC 6121). In the following sections we describe the observations of these clusters which were made using the TRIFFID camera between 1992 and 1995, the image sharpening and calibration steps performed, and the photometric techniques

  12. High-resolution imaging and crowded-field photometry of the stellar populations in the cores of the Globular Clusters M15 and M4

    NASA Astrophysics Data System (ADS)

    Butler, R. F.

    1999-02-01

    This thesis presents work performed at the Department of Physics, University College Galway from 1992 to 1997. It is concerned with ground- and space-based high-resolution optical imaging of globular cluster cores, and the subsequent application of image-restoration and crowded-field photometry techniques; thus we may gain an improved understanding of the nature of their stellar populations, by either monitoring their temporal behaviour over moderate periods for the first time, or by obtaining a more precise "static" picture than was hitherto possible. These goals can be achieved by the development of innovative instrumentation and data analysis techniques. The particularly unique aspect of this work is that it deals with the first application of two-dimensional photon-counting detectors (2D-PCDs) and post-exposure image sharpening (PEIS) for crowded-field photometry. The thesis starts by introducing some basic concepts and characteristics of globular clusters and the diverse stellar species which they contain, in particular those predicted to have formed as a result of dynamical processes in the cluster cores, and those which exhibit variability in emission over time. It then reviews the fields of high-resolution imaging through the turbulent atmosphere & image deconvolution, optical stellar photometry, and Hubble Space Telescope observing and data reduction, each concluded with a description of the systems used in the work reported here (for the HST chapter this involves photometry of WFPC2 (Wide Field & Planetary Camera 2) observations of M15 (NGC 7078) released into the archives in 1995). The core of the thesis begins with a review of the observations to date of the objects with which this thesis is chiefly concerned, M15 and M4 (NGC 6121). In the following sections we describe the observations of these clusters which were made using the TRIFFID camera between 1992 and 1995, the image sharpening and calibration steps performed, and the photometric techniques

  13. The divergent eukaryote Trichomonas vaginalis has an m7G cap methyltransferase capable of a single N2 methylation.

    PubMed

    Simoes-Barbosa, Augusto; Louly, Camila; Franco, Octávio L; Rubio, Mary A; Alfonzo, Juan D; Johnson, Patricia J

    2008-12-01

    Eukaryotic RNAs typically contain 5' cap structures that have been primarily studied in yeast and metazoa. The only known RNA cap structure in unicellular protists is the unusual Cap4 on Trypanosoma brucei mRNAs. We have found that T. vaginalis mRNAs are protected by a 5' cap structure, however, contrary to that typical for eukaryotes, T. vaginalis spliceosomal snRNAs lack a cap and may contain 5' monophophates. The distinctive 2,2,7-trimethylguanosine (TMG) cap structure usually found on snRNAs and snoRNAs is produced by hypermethylation of an m(7)G cap catalyzed by the enzyme trimethylguanosine synthase (Tgs). Here, we biochemically characterize the single T. vaginalis Tgs (TvTgs) encoded in its genome and demonstrate that TvTgs exhibits substrate specificity and amino acid requirements typical of an RNA cap-specific, m(7)G-dependent N2 methyltransferase. However, recombinant TvTgs is capable of catalysing only a single round of N2 methylation forming a 2,7-dimethylguanosine cap (DMG) as observed previously for Giardia lamblia. In contrast, recombinant Entamoeba histolytica and Trypanosoma brucei Tgs are capable of catalysing the formation of a TMG cap. These data suggest the presence of RNAs with a distinctive 5' DMG cap in Trichomonas and Giardia lineages that are absent in other protist lineages.

  14. The M=7.9 Alaska Earthquake of 3 November 2002: felt reports and unusual effects across western Canada

    NASA Astrophysics Data System (ADS)

    Bird, A. L.; Cassidy, J. F.; Rogers, G. C.; Mulder, T. L.

    2003-04-01

    The 3~November 2002, M=7.9 Alaska earthquake was one of the largest earthquakes recorded in North America during the past 100 years. This earthquake was located 330~km west of the Yukon-Alaska border; surface rupture and aftershocks extended to within about 100~km of the border. More than 250 ``felt'' reports were submitted to the Geological Survey of Canda website (http://www.pgc.nrcan.gc.ca) within a few days of the earthquake. We summarize those reports from across western Canada. Accounts include typical high-frequency shaking effects to distances of approximately 1500~km, as well as numerous long-period effects to distances of nearly 3500~km: humans experiencing nausea, swaying high-rise buildings, telephone poles and chandeliers, seiches in lakes, inlets and swimming pools, instances of dirty well-water.

  15. Acoustic Emission Precursors of M6.0 2004 Parkfield and M7.0 1989Loma Prieta Earthquakes

    SciTech Connect

    Korneev, Valeri

    2005-02-01

    Two recent strike-slip earthquakes on the San Andreas Fault(SAF) in California, the M6.0 2004 Parkfield and M7.0 1989 Loma Prietaevents, revealed peaks in the acoustic emission (AE) activity in thesurrounding crust several months prior to the main events. Earthquakesdirectly within the SAF zone were intentionally excluded from theanalysis. The observed increase in AE is assumed to be a signature of theincreasing stress level in the surrounding crust, while the peak andsubsequent decrease in AE starting several months prior to the mainevents is attributed to damage-induced softening processes as discussedherein. Further, distinctive zones of low seismic activity surroundingthe epicentral regions in the pre-event time period are present for thetwo studied events. Both AE increases in the crust surrounding apotential future event and the development of a low-seismicity epicentralzone can be regarded as promising precursory information that could helpsignal the arrival of large earthquakes.

  16. Coseismic and postseismic vertical movements associated with the 1940 M7.1 Imperial Valley, California, earthquake

    NASA Technical Reports Server (NTRS)

    Reilinger, R.

    1984-01-01

    Leveling surveys conducted along two routes that cross the Imperial fault in southern California indicate spatially coherent elevation changes attributable to coseismic and postseismic effects of the 1940, M7.1 Imperial Valley earthquake. The 1931-1941 elevation changes are consistent with theoretical models of vertical deformation of an elastic half space for a finite length strike-slip fault, using fault parameters that are consistent with the observed surface offsets following the 1940 earthquake. The elevation changes suggest an earthquake scenario consisting of a large coeismic slip in the southern half of the fault which transferred stress to the northern part as well as to the Brawley fault to the northeast.

  17. Escherichia coli-producing extended-spectrum beta-lactamase CTX-M-15 in a captive South American tapir (Tapirus terrestris).

    PubMed

    Klimes, Jiri; Machalkova, Marketa; Dolejska, Monika; Cizek, Alois; Janoszowska, Dagmar; Alexa, Pavel; Albrechtova, Katerina; Vojtech, Jiri; Literak, Ivan

    2013-03-01

    Only a few reports exist on the occurrence of resistant bacteria in zoo animals. Therefore, an isolation of multiresistant Escherichia coli from the lungs of a captive South American tapir (Tapirus terrestris) lead to its characterization and further investigation of samples from animals inhabiting the same paddock and from the shared environment. The tapir suffered from an intermandibular abscess and pneumonia and was euthanatized after unsuccessful therapy, including administration of antibiotics. The authors performed selective isolation of extended-spectrum beta-lactamase (ESBL)-positive E. coli strains and identification of resistance genes using polymerase chain reaction. Seven multiresistant, ESBL-producing E. coli isolates were obtained, all belonging to the B2 phylogenetic group and showing identical profile on pulsed-field gel electrophoresis. These isolates carried several resistance genes, including the gene bla(CTX-M-15). This case demonstrates the transmission of related epidemiologically important E. coli isolates whose potential transmission to other animals and zoo staff can be assumed. PMID:23505722

  18. Successful international clones of blaCTX-M-15-producing Klebsiella pneumoniae with coexpression of plasmid-mediated quinolone resistance (PMQR) determinants in Tehran hospitals.

    PubMed

    Nematzadeh, Shoeib; Shahcheraghi, Fereshteh; Iversen, Aina; Giske, Christian G

    2015-12-01

    The dissemination of plasmid-mediated multidrug resistance in Enterobacteriaceae is a major public health concern. We investigated the prevalence of plasmid-mediated quinolone resistance (PMQR), 16S rRNA methylases, CTX-M, and acquired AmpC enzymes in ESBL-producing Klebsiella pneumoniae (n=40) from Tehran hospitals. Plasmid replicon typing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were carried out for typing. CTX-M group 1 (confirmed as bla(CTX-M-15) in selected isolates) was found in 35/40 isolates. Thirty-two isolates hosted PMQR genes. Twenty isolates featured aac(6')-Ib-CR only; 9 isolates had aac(6')-Ib-CR and qnrB; 2 isolates had aac(6')-Ib-CR and qnrS; and 1 isolate had aac(6')-Ib-CR, qnrS, and qepA. The 16S rRNA methylase RmtB was found in 1 isolate; and acquired AmpC enzymes, in 6 isolates. PFGE detected 7 pulsotypes, the largest corresponded to sequence type 16. The successful clone ST101 was also found. The emergence of successful clones of K. pneumoniae in Tehran hospitals is concerning. PMID:26458278

  19. Escherichia coli-producing extended-spectrum beta-lactamase CTX-M-15 in a captive South American tapir (Tapirus terrestris).

    PubMed

    Klimes, Jiri; Machalkova, Marketa; Dolejska, Monika; Cizek, Alois; Janoszowska, Dagmar; Alexa, Pavel; Albrechtova, Katerina; Vojtech, Jiri; Literak, Ivan

    2013-03-01

    Only a few reports exist on the occurrence of resistant bacteria in zoo animals. Therefore, an isolation of multiresistant Escherichia coli from the lungs of a captive South American tapir (Tapirus terrestris) lead to its characterization and further investigation of samples from animals inhabiting the same paddock and from the shared environment. The tapir suffered from an intermandibular abscess and pneumonia and was euthanatized after unsuccessful therapy, including administration of antibiotics. The authors performed selective isolation of extended-spectrum beta-lactamase (ESBL)-positive E. coli strains and identification of resistance genes using polymerase chain reaction. Seven multiresistant, ESBL-producing E. coli isolates were obtained, all belonging to the B2 phylogenetic group and showing identical profile on pulsed-field gel electrophoresis. These isolates carried several resistance genes, including the gene bla(CTX-M-15). This case demonstrates the transmission of related epidemiologically important E. coli isolates whose potential transmission to other animals and zoo staff can be assumed.

  20. Successful international clones of blaCTX-M-15-producing Klebsiella pneumoniae with coexpression of plasmid-mediated quinolone resistance (PMQR) determinants in Tehran hospitals.

    PubMed

    Nematzadeh, Shoeib; Shahcheraghi, Fereshteh; Iversen, Aina; Giske, Christian G

    2015-12-01

    The dissemination of plasmid-mediated multidrug resistance in Enterobacteriaceae is a major public health concern. We investigated the prevalence of plasmid-mediated quinolone resistance (PMQR), 16S rRNA methylases, CTX-M, and acquired AmpC enzymes in ESBL-producing Klebsiella pneumoniae (n=40) from Tehran hospitals. Plasmid replicon typing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were carried out for typing. CTX-M group 1 (confirmed as bla(CTX-M-15) in selected isolates) was found in 35/40 isolates. Thirty-two isolates hosted PMQR genes. Twenty isolates featured aac(6')-Ib-CR only; 9 isolates had aac(6')-Ib-CR and qnrB; 2 isolates had aac(6')-Ib-CR and qnrS; and 1 isolate had aac(6')-Ib-CR, qnrS, and qepA. The 16S rRNA methylase RmtB was found in 1 isolate; and acquired AmpC enzymes, in 6 isolates. PFGE detected 7 pulsotypes, the largest corresponded to sequence type 16. The successful clone ST101 was also found. The emergence of successful clones of K. pneumoniae in Tehran hospitals is concerning.

  1. Antimicrobial susceptibility to parenteral and oral agents in a largely polyclonal collection of CTX-M-14 and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae.

    PubMed

    Titelman, Emilia; Iversen, Aina; Kahlmeter, Gunnar; Giske, Christian G

    2011-12-01

    Activity of oral and parenteral antimicrobials against consecutively isolated extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (n = 149) and Klebsiella pneumoniae (n = 20) was determined, and susceptibility test methods were compared for parenteral β-lactams. Polymerase chain reaction (PCR) targeting bla(CTX-M), bla(SHV) and bla(TEM), and DNA sequencing and epidemiological typing with pulsed-field gel electrophoresis were performed. PCR targeting pabB was screened for E. coli O25b-ST131. Minimum inhibitory concentrations (MICs) were determined using Etest and broth microdilution. Disc diffusion was performed according to European Committee on Antimicrobial Susceptibility Testing (EUCAST). Dominating genotypes were bla(CTX-M-15) (75%) and bla(CTX-M-14) (23%). Four E. coli clusters (7-18 isolates) were found. Forty-two per cent of E. coli belonged to O25b-ST131. Ciprofloxacin resistance was 72%, trimethoprim resistance was 70%. Among E. coli, resistance to mecillinam (13%), nitrofurantoin (7%) and fosfomycin (3%) was low, although resistance was high in K. pneumoniae (25%, 60%, 85%). Susceptibility to ertapenem was 99%, piperacillin-tazobactam 91%, tigecycline 96% and temocillin 76%. Susceptibility rates obtained with broth microdilution and Etest were in agreement for cefotaxime (2 vs 1%) and ceftazidime (9 vs 11%), but not for piperacillin-tazobactam (59 vs 91%). With disc diffusion major errors occurred with piperacillin-tazobactam (18/169). Several therapeutic alternatives exist for ESBL-producing E. coli, but few exist for K. pneumoniae. Disc diffusion and Etest can accurately predict susceptibility to cefotaxime and ceftazidime, but not to piperacillin-tazobactam with the present breakpoints. PMID:22085361

  2. Dissemination of IncFII(K)-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from children in hospital paediatric oncology wards.

    PubMed

    Dolejska, Monika; Brhelova, Eva; Dobiasova, Hana; Krivdova, Jana; Jurankova, Jana; Sevcikova, Alena; Dubska, Lenka; Literak, Ivan; Cizek, Alois; Vavrina, Martin; Kutnikova, Lucia; Sterba, Jaroslav

    2012-12-01

    In this study, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolates in children with malignancies hospitalised at a paediatric oncology department in the Czech Republic were investigated. From June 2009 to January 2010, a total of 50 ESBL-producing faecal isolates of Enterobacteriaceae were obtained from 28 patients. These isolates were characterised with regard to ESBL enzymes, plasmid-mediated quinolone resistance genes, multilocus sequence typing (MLST) and plasmids conferring resistance to cephalosporins and fluoroquinolones. ESBL-producing isolates included Klebsiella pneumoniae (n=36), Escherichia coli (n=7), Klebsiella oxytoca (n=3), Enterobacter cloacae (n=2) and Citrobacter freundii (n=2). Klebsiella pneumoniae isolates belonged to 7 MLST types, including sequence types ST280, ST321, ST323 and ST416 as well as the novel types ST626, ST627 and ST628. The multiresistant epidemic clone E. coli B2-O25b-ST131 was detected in one patient. The gene bla(CTX-M-15) was found on large conjugative IncFII(K) plasmids along with bla(TEM-1), bla(OXA-1), qnrB1, aac(6')-Ib-cr, strA, sul2, aac(3')-II and tet(A) genes in most isolates. Dissemination of IncFII(K) plasmids among various Enterobacteriaceae isolates was considered an important aspect of nosocomial colonisation in the wards by Enterobacteriaceae species producing ESBLs. This is the first study documenting multiple antibiotic resistance elements, including qnr genes, in IncFII(K) plasmids in various bacterial species isolated in a single hospital department. The results highlight the evolution of IncFII(K) plasmids into new variants containing novel antibiotic resistance elements and their important role in spreading ESBL-producing bacteria among hospitalised patients.

  3. Genomic and Functional Portrait of a Highly Virulent, CTX-M-15-Producing H30-Rx Subclone of Escherichia coli Sequence Type 131

    PubMed Central

    Ranjan, Amit; Shaik, Sabiha; Hussain, Arif; Nandanwar, Nishant; Semmler, Torsten; Jadhav, Savita; Wieler, Lothar H.

    2015-01-01

    Escherichia coli sequence type 131 (ST131) is a pandemic clone associated with multidrug-resistant, extraintestinal infections, attributable to the presence of the CTX-M-15 extended-spectrum β-lactamase gene and mutations entailing fluoroquinolone resistance. Studies on subclones within E. coli ST131 are critically required for targeting and implementation of successful control efforts. Our study comprehensively analyzed the genomic and functional attributes of the H30-Rx subclonal strains NA097 and NA114, belonging to the ST131 lineage. We carried out whole-genome sequencing, comparative analysis, phenotypic virulence assays, and profiling of the antibacterial responses of THP1 cells infected with these subclones. Phylogenomic analysis suggested that the strains were clonal in nature and confined entirely to a single clade. Comparative genomic analysis revealed that the virulence and resistance repertoires were comparable among the H30-Rx ST131 strains except for the commensal ST131 strain SE15. Similarly, seven phage-specific regions were found to be strongly associated with the H30-Rx strains but were largely absent in the genome of SE15. Phenotypic analysis confirmed the virulence and resistance similarities between the two strains. However, NA097 was found to be more robust than NA114 in terms of virulence gene carriage (dra operon), invasion ability (P < 0.05), and antimicrobial resistance (streptomycin resistance). RT2 gene expression profiling revealed generic upregulation of key proinflammatory responses in THP1 cells, irrespective of ST131 lineage status. In conclusion, our study provides comprehensive, genome-inferred insights into the biology and immunological properties of ST131 strains and suggests clonal diversification of genomic and phenotypic features within the H30-Rx subclone of E. coli ST131. PMID:26195517

  4. Outbreak of Serratia marcescens Coproducing ArmA and CTX-M-15 Mediated High Levels of Resistance to Aminoglycoside and Extended-Spectrum Beta-Lactamases, Algeria.

    PubMed

    Batah, Rima; Loucif, Lotfi; Olaitan, Abiola Olumuyiwa; Boutefnouchet, Nafissa; Allag, Hamoudi; Rolain, Jean-Marc

    2015-08-01

    Serratia marcescens is one of the most important pathogens responsible for nosocomial infections worldwide. Here, we have investigated the molecular support of antibiotic resistance and genetic relationships in a series of 54 S. marcescens clinical isolates collected from Eastern Algeria between December 2011 and July 2013. The 54 isolates were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Antibiotic susceptibility testing was performed by disc diffusion and E-test methods. Antibiotic resistance genes were detected by polymerase chain reaction (PCR). The genetic transfer of antibiotic resistance was performed by conjugation using azide-resistant Escherichia coli J53 as the recipient strain, and plasmid analysis was done by PCR-based replicon typing. The relatedness of our isolates was determined by phylogenetic analysis based on partial sequences of four protein-encoding genes (gyrB, rpoB, infB, and atpD) and then compared to MALDI-TOF MS clustering. Thirty-five out of 54 isolates yielded an extended-spectrum β-lactamase (ESBL) phenotype and carried bla(CTX-M-15) (n=32), bla(TEM-1) (n=26), bla(TEM-71) (n=1), bla(SHV-1a) (n=1), and bla(PER-2) (n=12). Among these isolates, we identified a cluster of 15 isolates from a urology unit that coharbored ESBL and the 16S rRNA methyltransferase armA. Conjugation was successful for five selected strains, demonstrating the transferability of a conjugative plasmid of incompatibility group incL/M type. Phylogenetic analysis along with MALDI-TOF clustering likely suggested an outbreak of such isolates in the urology unit. In this study, we report for the first time the co-occurrence of armA methyltransferase with ESBL in S. marcescens clinical isolates in Eastern Algeria.

  5. Post-Seismic Fault Healing on the Rupture Zone of the 1999 M7.1 Hector Mine, California Earthquake

    NASA Astrophysics Data System (ADS)

    Li, Y.; Vidale, J. E.; Day, S. M.; Oglesby, D. D.; Cochran, E.; Gross, K.; Burdette, T.; Alvarez, M.

    2002-12-01

    We probed the rupture zone of the October 1999 M7.1 Hector Mine earthquake using repeated near-surface explosions in October, 2000 and November, 2001. Three dense linear seismic arrays were deployed across the north and south Lavic Lake faults (LLF) that broke to the surface in the mainshock, and across the Bullion fault (BF) that experienced minor slip in that event. Two explosions each year were detonated in the rupture zone on the middle and south LLF, respectively. We found that P and S velocities of fault-zone rocks increased by ~0.7 to 1.4% and ~0.5 to 1.0% between 2000 and 2001, respectively. In contrast, the velocities for P and S waves in surrounding rocks increased much less. This trend indicates the Hector Mine rupture zone has been healing by strengthening after the mainshock, which we attribute to the closure of cracks that opened during the 1999 earthquake. The 'crack dilatancy' mechanisms are most likely to operate for fault healing at shallow depth although the healing may be controlled by a combination of mechanical and chemical processes on the fault during the earthquake cycle. The observed fault-zone strength recovery is consistent with an apparent crack density decrease of 1.5% within the rupture zone. The ratio of travel time decrease for P to S waves was 0.72, consistent with partially fluid-filled cracks near the fault zone were. We also find variability in healing rates between the fault segments. The velocity increase with time varies from one fault segment to another at the Hector Mine rupture zone. We see greater changes on the LLF than on the BF, and the greatest change is on the middle LLF at shallow depth. We speculate that greater damage was inflicted, and thus greater healing is observed, in regions with larger slip in the mainshock. This post-seismic restrengthening of the Hector Mine rupture zone is similar to that observed on the Johnson Valley fault which ruptured in the 1992 M7.4 Landers earthquake (Li and Vidale, GRL, 2001

  6. Rupture directivity and local site effects: the M7.3 Honduras earthquake of May 23, 2009

    NASA Astrophysics Data System (ADS)

    Shulman, D.; Mooney, W. D.

    2009-12-01

    On May 28, 2009, at 2:24 AM local time, a M 7.3 earthquake struck off the coast of Honduras on the Motagua-Swan Fault System (MSFS), part of the boundary between the North America and Caribbean plates. This plate boundary has an average slip rate of 20 mm/year. This left-lateral earthquake had an average slip of 1.5 m on a 100-km-long near-vertical fault plane (Hayes and Ji, 2009). The hypocenter depth is estimated at 10 km. The main shock caused 130 structures, including homes and office buildings, to collapse or suffer significant damage in northern Honduras. Seven deaths were reported. Due to a lack of recordings in the area, the available documentation of the local effects of this earthquake are the USGS "Did you feel it?" responses and the data collected during our field seismic intensity investigation. We conducted a field investigation in Honduras between May 30 and June 6, 2009, focused on areas with local reports of damage, including the cities of La Ceiba, El Progresso, San Pedro Sula, Puerto Cortes in northern Honduras and the island of Roatan in the Caribbean Sea. The damage ascertained at these five sites shows that the severity of damage did not decrease with distance from the epicenter as predicted by standard attenuation relations. Instead, a concentration of damage was observed in El Progresso, approximately 75 km directly south from the SW end of the rupture and 160 km from the epicenter. The island of Roatan, just 30 km from the epicenter, was graded as VI on the Modified Mercalli Intensity scale while, El Progresso was graded as VIII (one unit higher than “Did you feel it?”). These intensity anomalies can be explained by two factors: (1) SW-directed rupture propagation and proximity to a localized 3.0m slip pulse (asperity) that occurred near the SW end of the fault (Hayes and Ji, 2009) that focused energy toward the city of El Progress on the mainland and; (2) local site effects, particularly the Precambrian schists and gneisses on the

  7. The 1992 M=7 Cape Mendocino, California, earthquake: Coseismic deformation at the south end of the Cascadia megathrust

    USGS Publications Warehouse

    Murray, M.H.; Marshall, G.A.; Lisowski, M.; Stein, R.S.

    1996-01-01

    We invert geodetic measurements of coseismic surface displacements to determine a dislocation model for the April 25, 1992, M=7 Cape Mendocino, California, earthquake. The orientation of the model slip vector, which nearly parallels North America-Juan de Fuca relative plate convergence, and the location and orientation of the model fault relative to the offshore Cascadia megathrust, suggest that the 1992 Cape Mendocino earthquake is the first well-recorded event to relieve strain associated with the Cascadia subduction zone. We use data from three geodetic techniques: (1) the horizontal and vertical displacements of 13 monuments surveyed with the Global Positioning System, corrected for observed horizontal interseismic strain accumulation, (2) 88 section-elevation differences between leveling monuments, and (3) the uplift of 12 coastal sites observed from the die-off of intertidal marine organisms. Maximum observed displacements are 0.4 m of horizontal movement and 1.5 m of uplift along the coast. We use Monte Carlo techniques to estimate an optimal uniform slip rectangular fault geometry and its uncertainties. The optimal model using all the data resolves 4.9 m of slip on a 14 by 15 km fault that dips 28?? SE. The fault extends from 1.5 to 8.7 km in depth and the main-shock hypocenter is close to the downdip projection of the fault. The shallowly dipping fault plane is consistent with the observed aftershock locations, and the estimated geodetic moment is 3.1??1019 N m, 70% of the seismic moment. Other models that exclude leveling data collected in 1935 and 1942 are more consistent with seismological estimates of the fault geometry. If the earthquake is characteristic for this segment, the estimated horizontal slip vector compared with plate convergence rates suggests a recurrence interval of 140 years, with a 95% confidence range of 100-670 years. The coseismic uplift occurred in a region that also has high Quaternary uplift rates determined from marine terrace

  8. Identification and role analysis of an intermediate produced by a polygenic mutant of Monascus pigments cluster in Monascus ruber M7.

    PubMed

    Liu, Jiao; Zhou, Youxiang; Yi, Tao; Zhao, Mingming; Xie, Nana; Lei, Ming; Liu, Qingpei; Shao, Yanchun; Chen, Fusheng

    2016-08-01

    Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that the mutant named ΔMpigJ-R lost proximate eight genes of the Mps gene cluster in M. ruber M7 through genetic analysis at DNA and RNA levels. The produced light yellow material was identified as a benzaldehyde derivative named as 6-(4-hydroxy-2-oxopentyl)-3-methyl-2, 4-dioxocyclohexane carb-aldehyde (M7PKS-1) by FT-IR, NMR, and MS. The sodium acetate-1-(13)C feeding experiment indicated that M7PKS-1 was a product produced from polyketide pathway. Finally, the feeding of M7PKS-1 helped to induce and regain Mps production of the mutants (ΔMpigA and ΔMpigE) which were previously unable to biosynthesize Mps and proved that M7PKS-1 was an initial intermediate of Mps. The results in this study provide a line of action to unveil Monascus pigments biosynthesis pathway. PMID:26946170

  9. Lateral spread hazard mapping of the northern Salt Lake Valley, Utah, for a M7.0 scenario earthquake

    USGS Publications Warehouse

    Olsen, M.J.; Bartlett, S.F.; Solomon, B.J.

    2007-01-01

    This paper describes the methodology used to develop a lateral spread-displacement hazard map for northern Salt Lake Valley, Utah, using a scenario M7.0 earthquake occurring on the Salt Lake City segment of the Wasatch fault. The mapping effort is supported by a substantial amount of geotechnical, geologic, and topographic data compiled for the Salt Lake Valley, Utah. ArcGIS?? routines created for the mapping project then input this information to perform site-specific lateral spread analyses using methods developed by Bartlett and Youd (1992) and Youd et al. (2002) at individual borehole locations. The distributions of predicted lateral spread displacements from the boreholes located spatially within a geologic unit were subsequently used to map the hazard for that particular unit. The mapped displacement zones consist of low hazard (0-0.1 m), moderate hazard (0.1-0.3 m), high hazard (0.3-1.0 m), and very high hazard (> 1.0 m). As expected, the produced map shows the highest hazard in the alluvial deposits at the center of the valley and in sandy deposits close to the fault. This mapping effort is currently being applied to the southern part of the Salt Lake Valley, Utah, and probabilistic maps are being developed for the entire valley. ?? 2007, Earthquake Engineering Research Institute.

  10. Rapid earthquake characterization using MEMS accelerometers and volunteer hosts following the M 7.2 Darfield, New Zealand, Earthquake

    USGS Publications Warehouse

    Lawrence, J. F.; Cochran, E.S.; Chung, A.; Kaiser, A.; Christensen, C. M.; Allen, R.; Baker, J.W.; Fry, B.; Heaton, T.; Kilb, Debi; Kohler, M.D.; Taufer, M.

    2014-01-01

    We test the feasibility of rapidly detecting and characterizing earthquakes with the Quake‐Catcher Network (QCN) that connects low‐cost microelectromechanical systems accelerometers to a network of volunteer‐owned, Internet‐connected computers. Following the 3 September 2010 M 7.2 Darfield, New Zealand, earthquake we installed over 180 QCN sensors in the Christchurch region to record the aftershock sequence. The sensors are monitored continuously by the host computer and send trigger reports to the central server. The central server correlates incoming triggers to detect when an earthquake has occurred. The location and magnitude are then rapidly estimated from a minimal set of received ground‐motion parameters. Full seismic time series are typically not retrieved for tens of minutes or even hours after an event. We benchmark the QCN real‐time detection performance against the GNS Science GeoNet earthquake catalog. Under normal network operations, QCN detects and characterizes earthquakes within 9.1 s of the earthquake rupture and determines the magnitude within 1 magnitude unit of that reported in the GNS catalog for 90% of the detections.

  11. Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.

    PubMed

    Zegers, Ingrid; Gigot, Daniel; van Vliet, Françoise; Tricot, Catherine; Aymerich, Stéphane; Bujnicki, Janusz M; Kosinski, Jan; Droogmans, Louis

    2006-01-01

    The structure of Bacillus subtilis TrmB (BsTrmB), the tRNA (m7G46) methyltransferase, was determined at a resolution of 2.1 A. This is the first structure of a member of the TrmB family to be determined by X-ray crystallography. It reveals a unique variant of the Rossmann-fold methyltransferase (RFM) structure, with the N-terminal helix folded on the opposite site of the catalytic domain. The architecture of the active site and a computational docking model of BsTrmB in complex with the methyl group donor S-adenosyl-L-methionine and the tRNA substrate provide an explanation for results from mutagenesis studies of an orthologous enzyme from Escherichia coli (EcTrmB). However, unlike EcTrmB, BsTrmB is shown here to be dimeric both in the crystal and in solution. The dimer interface has a hydrophobic core and buries a potassium ion and five water molecules. The evolutionary analysis of the putative interface residues in the TrmB family suggests that homodimerization may be a specific feature of TrmBs from Bacilli, which may represent an early stage of evolution to an obligatory dimer.

  12. Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase

    PubMed Central

    Zegers, Ingrid; Gigot, Daniel; van Vliet, Françoise; Tricot, Catherine; Aymerich, Stéphane; Bujnicki, Janusz M.; Kosinski, Jan; Droogmans, Louis

    2006-01-01

    The structure of Bacillus subtilis TrmB (BsTrmB), the tRNA (m7G46) methyltransferase, was determined at a resolution of 2.1 Å. This is the first structure of a member of the TrmB family to be determined by X-ray crystallography. It reveals a unique variant of the Rossmann-fold methyltransferase (RFM) structure, with the N-terminal helix folded on the opposite site of the catalytic domain. The architecture of the active site and a computational docking model of BsTrmB in complex with the methyl group donor S-adenosyl-l-methionine and the tRNA substrate provide an explanation for results from mutagenesis studies of an orthologous enzyme from Escherichia coli (EcTrmB). However, unlike EcTrmB, BsTrmB is shown here to be dimeric both in the crystal and in solution. The dimer interface has a hydrophobic core and buries a potassium ion and five water molecules. The evolutionary analysis of the putative interface residues in the TrmB family suggests that homodimerization may be a specific feature of TrmBs from Bacilli, which may represent an early stage of evolution to an obligatory dimer. PMID:16600901

  13. Monacyclinones, New Angucyclinone Metabolites Isolated from Streptomyces sp. M7_15 Associated with the Puerto Rican Sponge Scopalina ruetzleri

    PubMed Central

    Vicente, Jan; Stewart, Allison K.; van Wagoner, Ryan M.; Elliott, Elizabeth; Bourdelais, Andrea J.; Wright, Jeffrey L. C.

    2015-01-01

    During an investigation of new actinomycete species from Caribbean sponges for novel bioactive natural products, frigocyclinone (1), dimethyldehydrorabelomycin (3) and six new angucyclinone derivatives were isolated from Streptomyces sp. strain M7_15 associated with the sponge Scopalina ruetzleri. Of these, monacyclinones A–B (4–5) contain the core ring structure of dehydrorabelomycin (2) with the aminodeoxysugar found in frigocyclinone (1). Monacyclinone C (6) is a hydroxylated variant of frigocyclinone (1) and monacyclinone D (7) is a Baeyer Villiger derivative of (6) which also exists as the open chain hydrolysis product monacyclinone E (8). Monacyclinone F (9) contains two unique epoxide rings attached to the angucyclinone moiety and an additional aminodeoxysugar attached through an angular oxygen bond. All structures were confirmed through spectral analyses. Activity against rhabdomycosarcoma cancer cells (SJCRH30) after 48 h of treatment was observed with frigocyclinone (1; EC50 = 5.2 µM), monacyclinone C (6; 160 µM), monacyclinone E (8; 270 µM), and monacyclinone F (9; 0.73 µM). The strongest bioactivity against rhabdomycosarcoma cancer cells and gram-positive bacteria was exhibited by compound 9, suggesting that the extra aminodeoxysugar subunit is important for biological activity. PMID:26230704

  14. Large rock avalanches triggered by the M 7.9 Denali Fault, Alaska, earthquake of 3 November 2002

    USGS Publications Warehouse

    Jibson, R.W.; Harp, E.L.; Schulz, W.; Keefer, D.K.

    2006-01-01

    The moment magnitude (M) 7.9 Denali Fault, Alaska, earthquake of 3 November 2002 triggered thousands of landslides, primarily rock falls and rock slides, that ranged in volume from rock falls of a few cubic meters to rock avalanches having volumes as great as 20 ?? 106 m3. The pattern of landsliding was unusual: the number and concentration of triggered slides was much less than expected for an earthquake of this magnitude, and the landslides were concentrated in a narrow zone about 30-km wide that straddled the fault-rupture zone over its entire 300-km length. Despite the overall sparse landslide concentration, the earthquake triggered several large rock avalanches that clustered along the western third of the rupture zone where acceleration levels and ground-shaking frequencies are thought to have been the highest. Inferences about near-field strong-shaking characteristics drawn from interpretation of the landslide distribution are strikingly consistent with results of recent inversion modeling that indicate that high-frequency energy generation was greatest in the western part of the fault-rupture zone and decreased markedly to the east. ?? 2005 Elsevier B.V. All rights reserved.

  15. Landslides and liquefaction triggered by the M 7.9 denali fault earthquake of 3 November 2002

    USGS Publications Warehouse

    Harp, E.L.; Jibson, R.W.; Kayen, R.E.; Keefer, D.K.; Sherrod, B.L.; Carver, G.A.; Collins, B.D.; Moss, R.E.S.; Sitar, N.

    2003-01-01

    The moment magnitude (M) 7.9 Denali Fault earthquake in Alaska of 3 November 2002 triggered an unusual pattern of landslides and liquefaction effects. The landslides were primarily rock falls and rock slides that ranged in volume from a few cubic meters to the 40 million-cubic-meter rock avalanche that covered much of the McGinnis Glacier. Landslides were concentrated in a narrow zone ???30 km wide that straddled the fault rupture zone over its entire 300 km length. Large rock avalanches all clustered at the western end of the rupture zone where acceleration levels are reported to have been the highest. Liquefaction effects, consisting of sand blows, lateral spreads, and settlement, were widespread within susceptible alluvial deposits extending from Fairbanks eastward several hundred kilometers. The liquefaction effects displayed a pattern of increasing concentration and severity from west to east and extended well beyond the zone of landslides, which is unusual. The contrasting patterns formed by the distributions of landslides and liquefaction effects initially seemed to be inconsistent; however, preliminary analyses of strong-motion records from the earthquake offer a possible explanation for the unusual ground-failure patterns that are related to three subevents that have been discerned from the earthquake records.

  16. Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes: variants M7 and DR1.

    PubMed

    Krangel, M S; Taketani, S; Biddison, W E; Strong, D M; Strominger, J L

    1982-11-23

    Comparative primary structural analyses have begun to elucidate polymorphic residues and segments of the class I antigens of the major histocompatibility complex, at least some of which presumably contribute to determinants important in immune recognition events. HLA-A2 structural variants have been described which are serologically indistinguishable from other HLA-A2 antigens, yet which can be recognized neither by HLA-A2 specific alloimmune nor by HLA-A2 restricted, virus immune cytotoxic T lymphocytes. This study utilizes double-label tryptic peptide comparisons in combination with both conventional and microsequence analyses to investigate the structure of two such variants, M7 and DR1. We find that these variants are identical with each other and differ from the predominant HLA-A2 heavy chain species by a glutamine to arginine substitution at residue 43, by an unidentified substitution in the tryptic peptide spanning residues 147-157, and by an as yet poorly defined alteration in glycosylation. Structural information from these and other variants should be useful in precisely defining functionally important determinants on the molecule. PMID:6983890

  17. Seismicity Precursors of the M6.0 2004 Parkfield and M7.0 1989Loma Prieta Earthquakes

    SciTech Connect

    Korneev, Valeri A.

    2006-03-09

    The M6.0 2004 Parkfield and M7.0 1989 Loma Prietastrike-slip earthquakes on the San Andreas Fault (SAF) were preceded byseismicity peaks occurring several months prior to the main events.Earthquakes directly within the SAF zone were intentionally excluded fromthe analysis because they manifest stress-release processes rather thanstress accumulation. The observed increase in seismicity is interpretedas a signature of the increasing stress level in the surrounding crust,whereas the peaks and the subsequent decrease in seismicity areattributed to damage-induced softening processes. Furthermore, in bothcases there is a distinctive zone of low seismic activity that surroundsthe epicentral region in the pre-event period. The increase of seismicityin the crust surrounding a potential future event and the development ofa low-seismicity epicentral zone can be regarded as promising precursoryinformation that could help signal the arrival of large earthquakes. TheGutenberg-Richter relationship (GRR) should allow extrapolation ofseismicity changes down to seismic noise level magnitudes. Thishypothesis is verified by comparison of seismic noise at 80 Hz with theParkfield M4 1993-1994 series, where noise peaks 5 months before theseries to about twice the background level.

  18. Growth of a Structure Connecting the 2010 M 7.2 El Mayor - Cucapah Rupture with the Elsinore Faul

    NASA Astrophysics Data System (ADS)

    Donnellan, A.; Parker, J. W.

    2015-12-01

    The M 7.2 El Mayor - Cucapah earthquake occurred on 4 April 2010 in the northern part of Baja, Mexico. The rupture extended about 120 km from near the northern tip of the Gulf of California to the US - Mexican border south of the Elsinore fault zone. Most of the aftershocks occurred within days of the main event. On 14 June 2010 a M 5.7 late aftershock occurred 8 km southeast of Ocotillo, CA and is the largest aftershock in the sequence. The right-lateral event occurred in a cluster of aftershocks and was followed by its own aftershock sequence. UAVSAR data were collected for a swath covering the aftershock on 13 April, 2010 just after the El Mayor - Cucapah earthquake and before the earthquake on 21 October 2009. The line was reflown 1 July 2010 after the M 5.7 14 June 2010 aftershock. Data have been continued to be collected semi yearly to yearly since then. Repeat Pass Interferomety (RPI) products spanning the aftershock show the growth of a lineament that with an azimuth of 121.5° or a strike of -58.5°. The interferograms suggest that a stepover develops following the earthquake. The epicenter of the M 5.7 aftershock is proximal to the linear discontinuity in the postseismic interferogram and the mechanism of the event is consistent with slip on this stepover. Inversions for slip on the northeast linear structure that steps west of the mainshock rupture yield a moment magnitude ranging from 5.5 - 5.8, which is consistent with the magnitude of the aftershock. Slip occurs at a depth of 2-10 km on a steeply dipping fault.

  19. Exponential decline of aftershocks of the M7.9 1868 great Kau earthquake, Hawaii, through the 20th century

    USGS Publications Warehouse

    Klein, F.W.; Wright, Tim

    2008-01-01

    The remarkable catalog of Hawaiian earthquakes going back to the 1820s is based on missionary diaries, newspaper accounts, and instrumental records and spans the great M7.9 Kau earthquake of April 1868 and its aftershock sequence. The earthquake record since 1868 defines a smooth curve complete to M5.2 of the declining rate into the 21st century, after five short volcanic swarms are removed. A single aftershock curve fits the earthquake record, even with numerous M6 and 7 main shocks and eruptions. The timing of some moderate earthquakes may be controlled by magmatic stresses, but their overall long-term rate reflects one of aftershocks of the Kau earthquake. The 1868 earthquake is, therefore, the largest and most controlling stress event in the 19th and 20th centuries. We fit both the modified Omori (power law) and stretched exponential (SE) functions to the earthquakes. We found that the modified Omori law is a good fit to the M ??? 5.2 earthquake rate for the first 10 years or so and the more rapidly declining SE function fits better thereafter, as supported by three statistical tests. The switch to exponential decay suggests that a possible change in aftershock physics may occur from rate and state fault friction, with no change in the stress rate, to viscoelastic stress relaxation. The 61-year exponential decay constant is at the upper end of the range of geodetic relaxation times seen after other global earthquakes. Modeling deformation in Hawaii is beyond the scope of this paper, but a simple interpretation of the decay suggests an effective viscosity of 1019 to 1020 Pa s pertains in the volcanic spreading of Hawaii's flanks. The rapid decline in earthquake rate poses questions for seismic hazard estimates in an area that is cited as one of the most hazardous in the United States.

  20. The Abundances of Neutron-capture Species in the Very Metal-poor Globular Cluster M15: A Uniform Analysis of Red Giant Branch and Red Horizontal Branch Stars

    NASA Astrophysics Data System (ADS)

    Sobeck, Jennifer S.; Kraft, Robert P.; Sneden, Christopher; Preston, George W.; Cowan, John J.; Smith, Graeme H.; Thompson, Ian B.; Shectman, Stephen A.; Burley, Gregory S.

    2011-06-01

    The globular cluster M15 is unique in its display of star-to-star variations in the neutron-capture elements. Comprehensive abundance surveys have been previously conducted for handfuls of M15 red giant branch (RGB) and red horizontal branch (RHB) stars. No attempt has been made to perform a single, self-consistent analysis of these stars, which exhibit a wide range in atmospheric parameters. In the current effort, a new comparative abundance derivation is presented for three RGB and six RHB members of the cluster. The analysis employs an updated version of the line transfer code MOOG, which now appropriately treats coherent, isotropic scattering. The apparent discrepancy in the previously reported values for the metallicity of M15 RGB and RHB stars is addressed and a resolute disparity of Δ(RHB - RGB) ≈ 0.1 dex in the iron abundance was found. The anti-correlative behavior of the light neutron-capture elements (Sr, Y, Zr) is clearly demonstrated with both Ba and Eu, standard markers of the s- and r-process, respectively. No conclusive detection of Pb was made in the RGB targets. Consequently for the M15 cluster, this suggests that the main component of the s-process has made a negligible contribution to those elements normally dominated by this process in solar system material. Additionally for the M15 sample, a large Eu abundance spread is confirmed, which is comparable to that of the halo field at the same metallicity. These abundance results are considered in the discussion of the chemical inhomogeneity and nucleosynthetic history of M15.

  1. First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria.

    PubMed

    Loucif, Lotfi; Gacemi-Kirane, Djamila; Cherak, Zineb; Chamlal, Naima; Grainat, Nadia; Rolain, Jean-Marc

    2016-10-01

    Here we report the isolation of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae from German cockroaches caught in the burn unit of Batna University Hospital in Algeria. Nine of 12 isolates harbored the blaCTX-M-15 ESBL gene. One Enterobacter cloacae isolate belonging to sequence type 528 coexpressed the blaOXA-48, blaCTX-M-15, and blaTEM genes. Our findings indicate that cockroaches may be one of the most dangerous reservoirs for ESBL and carbapenemase producers in hospitals.

  2. The M=7.9 Alaska Earthquake of 3 November 2002: Felt Reports and Unusual Effects Across Western Canada

    NASA Astrophysics Data System (ADS)

    Cassidy, J. F.; Rogers, G. C.; Bird, A. L.; Mulder, T. L.

    2002-12-01

    The 3 November 2002 M=7.9 Alaska earthquake was one of the largest earthquakes recorded in North America during the past 100 years. This earthquake occurred at 2:12 p.m. PST (on a Sunday) and was located 330 km to the west of the Yukon-Alaska border. Surface rupture and aftershocks extended to within about 100 km of the Canadian border. More than 250 "felt" reports were submitted to the Geological Survey of Canada website (http://www.pgc.nrcan.gc.ca/seismo/table.htm) within a few days of the earthquake. Here, we summarize those reports which include typical high-frequency shaking effects to distances of about 1500 km, as well as numerous long-period effects, such as human effects (nausea), swaying highrises, telephone poles and chandeliers, seiches in lakes and inlets, water sloshing from swimming pools, and instances of dirty well-water to distances of nearly 3500 km across Western Canada. Felt intensities (MMI)of about IV were observed across the Yukon Territory at distances of 350 km to 750 km. There were a few reports of minor damage in this region, as well as numerous reports of items knocked from shelves and parked vehicles rocking noticeably. The most distant felt reports in western Canada were from southern Alberta (2400 km distance) where people in highrises felt the swaying. More than 30 reports of human effects were received. These ranged from people feeling dizzy, seasick or nauseated (to distances of 2400 km), to difficulty standing and maintaining balance (to distances of 1000 km). Long-period effects of houses "swaying", large signs flexing, and telephone poles and tall trees swaying were reported to distances of more than 1000 km. Swinging of chandeliers, hanging plants and lights were reported to distances of 2400 km. There were more than 30 reports of seiches. Most reports came from southern British Columbia (2200-2400 km) where, although no ground shaking was noticed, water surges up to 1 m were observed. In one case a cabin held by cables near

  3. The first recorded surface rupture in southern Africa: the 22 February 2006 M 7.0 Machaze, Mozambique, earthquake

    NASA Astrophysics Data System (ADS)

    Fenton, C. H.

    2006-12-01

    A major (M 7.0) earthquake occurred on 22 February 2006 at 22:19 UTC in the western province of Manica in Mozambique, southern Africa. The epicenter was located in Machaze, the southernmost district of Manica province, about 45 km due south of the district capital of Chitobe. The earthquake was felt throughout eastern southern Africa but caused surprisingly little damage and a very small number of casualties. Surface rupture was mapped along the NNW-striking Machaze fault and liquefaction was observed throughout the epicentral region. This is the largest earthquake to have occurred in Mozambique in historical time. The surface-rupture along the Machaze fault is generally linear, without significant topographic deviation, indicating that the fault plane is relatively steep. Displacement is normal, down-to-the-west on west-dipping fault plane. The fault rupture is marked by a series of west-facing fault scarps developed in Holocene sandy alluvium. Vertical separation across the fault scarp ranges between 0.4 m and 2.05 m. At one location, pre- existing trails crossing the fault form piercing points that indicate a component of left-lateral strike-slip displacement of up to 0.7 m. To date we have only observed part of the surface rupture along a fault length of 15 km. Time constraints and the presence of minefields have prevented full investigation of the surface rupture. The total rupture length is estimated to be on the order of 30-40 km based on the regression equations between earthquake magnitude and surface rupture length and from the time function duration from preliminary waveform inversion for the mainshock. The surface rupture was developed in a broad, flat alluvial plain, with no apparent pre-existing fault-related geomorphology. However, with the benefit of hindsight, very subdued features, including vegetation lineaments and areas of ponded sediment that appear to define a lineament along part of the trace of the surface rupture are observed on pre

  4. Early post-seismic deformation due to the 2015 M7.8 Gorkha (Nepal) earthquake: Constraints on rheology of the Tibetan lithosphere

    NASA Astrophysics Data System (ADS)

    Fialko, Y. A.; Wang, K.

    2015-12-01

    Over the last decade several major earthquakes have occurred on faultsbounding the Tibetan plateau, including the 2005 M7.6 Kashmir(Pakistan), the 2008 M7.8 Wenchuan (China), and the 2015 M7.8 Gorkha(Nepal) earthquakes. Time-dependent surface deformation followingthese earthquakes provides important constraints on the effectiverheology of the lower crust and upper mantle beneath Tibet. Theepicentral area of the 2015 Gorkha (Nepal) earthquake has beenexceptionally well imaged by several Interferometric SyntheticAperture Radar (InSAR) satellites including ALOS-2 (Japanese SpaceAgency) and Sentinel-1 (European Space Agency) missions. We willpresent observations of surface deformation in the early (severalmonths) postseismic period following the 2015 Gorkha earthquake, andcompare surface deformation data to numerical models of postseismictransients assuming various relaxation mechanisms. We will alsocompare the postseismic deformation data from different earthquakes atthe boundaries of the Tibetan Plateau to evaluate the effects oflateral heterogeneities in rheological properties of the ductilesubstrate.

  5. Expression of M6 and M7 lysin in Mytilus edulis is not restricted to sperm, but occurs also in oocytes and somatic tissue of males and females.

    PubMed

    Heß, Anne-Katrin; Bartel, Manuela; Roth, Karina; Messerschmidt, Katrin; Heilmann, Katja; Kenchington, Ellen; Micheel, Burkhard; Stuckas, Heiko

    2012-08-01

    Sperm proteins of marine sessile invertebrates have been extensively studied to understand the molecular basis of reproductive isolation. Apart from molecules such as bindin of sea urchins or lysin of abalone species, the acrosomal protein M7 lysin of Mytilus edulis has been analyzed. M7 lysin was found to be under positive selection, but mechanisms driving the evolution of this protein are not fully understood. To explore functional aspects, this study investigated the protein expression pattern of M7 and M6 lysin in gametes and somatic tissue of male and female M. edulis. The study employs a previously published monoclonal antibody (G26-AG8) to investigate M6 and M7 lysin protein expression, and explores expression of both genes. It is shown that these proteins and their encoding genes are expressed in gametes and somatic tissue of both sexes. This is in contrast to sea urchin bindin and abalone lysin, in which gene expression is strictly limited to males. Although future studies need to clarify the functional importance of both acrosomal proteins in male and female somatic tissue, new insights into the evolution of sperm proteins in marine sessile invertebrates are possible. This is because proteins with male-specific expression (bindin, lysin) might evolve differently than proteins with expression in both sexes (M6/M7 lysin), and the putative function of both proteins in females opens the possibility that the evolution of M6/M7 lysin is under sexual antagonistic selection, for example, mutations beneficial to the acrosomal function that are less beneficial the function in somatic tissue of females. PMID:22674895

  6. Occurrence of CTX-M-3, CTX-M-15, CTX-M-14, and CTX-M-9 Extended-Spectrum β-Lactamases in Enterobacteriaceae Clinical Isolates in Korea

    PubMed Central

    Kim, Jungmin; Lim, Yu-Mi; Jeong, Young-Sook; Seol, Sung-Yong

    2005-01-01

    Among 603 isolates of Enterobacteriaceae collected between June and November 2003 from three university hospitals within Korea, blaCTX-M-3, blaCTX-M-15, blaCTX-M-14, and blaCTX-M-9 were detected in 41 isolates of species from five different genera of Enterobacteriaceae, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter spp., and Serratia marcescens. PMID:15793142

  7. The 2007 M7.7 Tocopilla northern Chile earthquake sequence - along and across strike rupture segmentation

    NASA Astrophysics Data System (ADS)

    Schurr, B.; Asch, G.; Motagh, M.; Oncken, O.; Chong Diaz, G.; Barrientos, S. E.; Vilotte, J.

    2010-12-01

    In November 2007 a M7.7 earthquake occurred near the coastal town of Tocopilla in the southern part of a presumed seismic gap extending some 500 km along the northern Chile subduction zone. This major segment last broke in a magnitude ≧8.5 earthquake in 1877. Assuming a complete lock of the interface, it has accumulated more than 8 m of slip deficit. The contiguous segments to the north and south broke in M≧8 earthquakes in 2001 and 1995. Teams from Chile (Universidad Católica del Norte and Universidad de Chile), France (IPGP) and Germany (GFZ) started in 2006 to install semi-permanent multi-parameter observatories within the Integrated Plate Boundary Observatory Chile (IPOC) Initiative to monitor deformation at a variety of spatial and temporal scales in the final stage of the seismic cycle. At the time of the Tocopilla earthquake, 12 sites were equipped with seismic broadband and strong-motion sensors recording both the mainshock and its aftershock series. The earthquake rupture extended for about 160 km from the centre of the Mejillones peninsula (MP) to about 20 km north of the town of Tocopilla. Slip was confined to the depth range 30-55 km and concentrated in two patches in the north and south with a maximum of about 2.6 m. Hence the earthquake released only a fraction of the slip deficit and broke only the down-dip part of the plate interface, with the up-dip limit of the rupture approximately following the coastline. This poses the important question why rupture did not extend offshore, where the interface is presumably locked based on models of long-term interseismic deformation. We relocated more than 1000 aftershocks occurring in the week following the mainshock using hand-picked arrival times, cross-correlation based differential travel times and the double-difference algorithm. Despite the sparseness of the network, the aftershocks sharply define the plate interface. Seismicity in the first 24h is congruent to the slip distribution with the area

  8. Stress rotations due to the M6.5 foreshock and M7.3 main shock in the 2016 Kumamoto, SW Japan, earthquake sequence

    NASA Astrophysics Data System (ADS)

    Yoshida, Keisuke; Hasegawa, Akira; Saito, Tatsuhiko; Asano, Youichi; Tanaka, Sachiko; Sawazaki, Kaoru; Urata, Yumi; Fukuyama, Eiichi

    2016-10-01

    A shallow M7.3 event with a M6.5 foreshock occurred along the Futagawa-Hinagu fault zone in Kyushu, SW Japan. We investigated the spatiotemporal variation of the stress orientations in and around the source area of this 2016 Kumamoto earthquake sequence by inverting 1218 focal mechanisms. The results show that the σ3 axis in the vicinity of the fault plane significantly rotated counterclockwise after the M6.5 foreshock and rotated clockwise after the M7.3 main shock in the Hinagu fault segment. This observation indicates that a significant portion of the shear stress was released both by the M6.5 foreshock and M7.3 main shock. It is estimated that the stress release by the M6.5 foreshock occurred in the shallower part of the Hinagu fault segment, which brought the stress concentration in its deeper part. This might have caused the M7.3 main shock rupture mainly along the deeper part of the Hinagu fault segment after 28 h.

  9. Constraints on the Distance Moduli, Helium and Metal Abundances, and Ages of Globular Clusters from their RR Lyrae and Non-variable Horizontal-branch Stars. I. M3, M15, and M92

    NASA Astrophysics Data System (ADS)

    VandenBerg, Don A.; Denissenkov, P. A.; Catelan, Márcio

    2016-08-01

    Up-to-date isochrones, zero-age horizontal-branch (ZAHB) loci, and evolutionary tracks for core He-burning stars are applied to the color-magnitude diagrams of M3, M15, and M92, focusing in particular on their RR Lyrae populations. Periods for the ab- and c-type variables are calculated using the latest theoretical calibrations of {log} {P}{ab} and {log} {P}c as a function of luminosity, mass, effective temperature ({T}{{eff}}), and metallicity. Our models are generally able to reproduce the measured periods to well within the uncertainties implied by the stellar properties on which pulsation periods depend, as well as the mean periods and cluster-to-cluster differences in < {P}{ab}> and < {P}c> , on the assumption of well-supported values of E(B-V), {(m-M)}V, and [Fe/H]. While many of RR Lyrae in M3 lie close to the same ZAHB that fits the faintest horizontal-branch (HB) stars at bluer or redder colors, the M92 variables are all significantly evolved stars from ZAHB locations on the blue side of the instability strip. M15 appears to contain a similar population of HB stars as M92, along with additional helium-enhanced populations not present in the latter which comprise most of its RR Lyrae stars. The large number of variables in M15 and the similarity of the observed values of < {P}{ab}> and < {P}c> in M15 and M92 can be explained by HB models that allow for variations in Y. Similar ages (˜12.5 Gyr) are found for all three clusters, making them significantly younger than the field halo subgiant HD 140283. Our analysis suggests a preference for stellar models that take diffusive processes into account.

  10. Constraints on the Distance Moduli, Helium and Metal Abundances, and Ages of Globular Clusters from their RR Lyrae and Non-variable Horizontal-branch Stars. I. M3, M15, and M92

    NASA Astrophysics Data System (ADS)

    VandenBerg, Don A.; Denissenkov, P. A.; Catelan, Márcio

    2016-08-01

    Up-to-date isochrones, zero-age horizontal-branch (ZAHB) loci, and evolutionary tracks for core He-burning stars are applied to the color–magnitude diagrams of M3, M15, and M92, focusing in particular on their RR Lyrae populations. Periods for the ab- and c-type variables are calculated using the latest theoretical calibrations of {log} {P}{ab} and {log} {P}c as a function of luminosity, mass, effective temperature ({T}{{eff}}), and metallicity. Our models are generally able to reproduce the measured periods to well within the uncertainties implied by the stellar properties on which pulsation periods depend, as well as the mean periods and cluster-to-cluster differences in < {P}{ab}> and < {P}c> , on the assumption of well-supported values of E(B-V), {(m-M)}V, and [Fe/H]. While many of RR Lyrae in M3 lie close to the same ZAHB that fits the faintest horizontal-branch (HB) stars at bluer or redder colors, the M92 variables are all significantly evolved stars from ZAHB locations on the blue side of the instability strip. M15 appears to contain a similar population of HB stars as M92, along with additional helium-enhanced populations not present in the latter which comprise most of its RR Lyrae stars. The large number of variables in M15 and the similarity of the observed values of < {P}{ab}> and < {P}c> in M15 and M92 can be explained by HB models that allow for variations in Y. Similar ages (˜12.5 Gyr) are found for all three clusters, making them significantly younger than the field halo subgiant HD 140283. Our analysis suggests a preference for stellar models that take diffusive processes into account.

  11. Residues Distal to the Active Site Contribute to Enhanced Catalytic Activity of Variant and Hybrid β-Lactamases Derived from CTX-M-14 and CTX-M-15

    PubMed Central

    He, Dandan; Chiou, Jiachi; Zeng, Zhenling; Liu, Lanping; Chen, Xiaojie; Zeng, Li; Chan, Edward Wai Chi

    2015-01-01

    A variety of CTX-M-type extended-spectrum β-lactamases (ESBLs), including hybrid ones, have been reported in China that are uncommon elsewhere. To better characterize the substrate profiles and enzymatic mechanisms of these enzymes, we performed comparative kinetic analyses of both parental and hybrid CTX-M enzymes, including CTX-M-15, -132, -123, -64, -14 and -55, that are known to confer variable levels of β-lactam resistance in the host strains. All tested enzymes were susceptible to serine β-lactamase inhibitors, with sulbactam exhibiting the weakest inhibitory effects. CTX-M-55, which differs from CTX-M-15 by one substitution, A77V, displayed enhanced catalytic activity (kcat/Km) against expanded-spectrum cephalosporins (ESCs). CTX-M-55 exhibits higher structure stability, most likely by forming hydrophobic interactions between A77V and various key residues in different helices, thereby stabilizing the core architecture of the helix cluster, and indirectly contributes to a more stable active site conformation, which in turn shows higher catalytic efficiency and is more tolerant to temperature change. Analyses of the hybrids and their parental prototypes showed that evolution from CTX-M-15 to CTX-M-132, CTX-M-123, and CTX-M-64, characterized by gradual enhancement of catalytic activity to ESCs, was attributed to introduction of different substitutions to amino acids distal to the active site of CTX-M-15. Similarly, the increased hydrolytic activities against cephalosporins and sensitivity to β-lactamase inhibitors, clavulanic acid and sulbactam, of CTX-M-64 were partly due to the amino acids that were different from CTX-M-14 and located at both the C and N termini of CTX-M-64. These data indicate that residues distal to the active site of CTX-Ms contributed to their enhanced catalytic activities to ESCs. PMID:26169409

  12. Full-Genome Sequence of Escherichia coli K-15KW01, a Uropathogenic E. coli B2 Sequence Type 127 Isolate Harboring a Chromosomally Carried blaCTX-M-15 Gene.

    PubMed

    Zurfluh, Katrin; Tasara, Taurai; Stephan, Roger

    2016-01-01

    We present here the full-genome sequence of Escherichia coli K-15KW01, an extended-spectrum-β-lactamase-producing uropathogenic strain. Assembly and annotation of the draft genome resulted in a 5,154,641-bp chromosome and revealed a chromosomally contained blaCTX-M-15 gene embedded at the right-hand extremity of an ISEcp1 element in a plasmid-like structure (36,907 bp). PMID:27587831

  13. Full-Genome Sequence of Escherichia coli K-15KW01, a Uropathogenic E. coli B2 Sequence Type 127 Isolate Harboring a Chromosomally Carried blaCTX-M-15 Gene

    PubMed Central

    Zurfluh, Katrin; Tasara, Taurai

    2016-01-01

    We present here the full-genome sequence of Escherichia coli K-15KW01, an extended-spectrum-β-lactamase-producing uropathogenic strain. Assembly and annotation of the draft genome resulted in a 5,154,641-bp chromosome and revealed a chromosomally contained blaCTX-M-15 gene embedded at the right-hand extremity of an ISEcp1 element in a plasmid-like structure (36,907 bp). PMID:27587831

  14. Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis.

    PubMed

    Purta, Elzbieta; van Vliet, Françoise; Tricot, Catherine; De Bie, Lara G; Feder, Marcin; Skowronek, Krzysztof; Droogmans, Louis; Bujnicki, Janusz M

    2005-05-15

    The Escherichia coli TrmB protein and its Saccharomyces cerevisiae ortholog Trm8p catalyze the S-adenosyl-L-methionine-dependent formation of 7-methylguanosine at position 46 (m7G46) in tRNA. To learn more about the sequence-structure-function relationships of these enzymes we carried out a thorough bioinformatics analysis of the tRNA:m7G methyltransferase (MTase) family to predict sequence regions and individual amino acid residues that may be important for the interactions between the MTase and the tRNA substrate, in particular the target guanosine 46. We used site-directed mutagenesis to construct a series of alanine substitutions and tested the activity of the mutants to elucidate the catalytic and tRNA-recognition mechanism of TrmB. The functional analysis of the mutants, together with the homology model of the TrmB structure and the results of the phylogenetic analysis, revealed the crucial residues for the formation of the substrate-binding site and the catalytic center in tRNA:m7G MTases.

  15. A New View on the Space-Time Pattern of M>7 Earthquakes in the Northern Japan to Kurile Subduction Zones

    NASA Astrophysics Data System (ADS)

    Harada, T.; Satake, K.

    2012-04-01

    The northern Japan to southern Kurile trenches have been regarded as a typical subduction zone with spatially and temporally regular recurrence of great (M>8) interplate earthquakes. The source regions were grouped into six segments by Utsu (1972; 1984). The Headquarters for Earthquake Research Promotion of the Japanese government (2004) divided the southern Kurile subduction zone into four regions and evaluated future probabilities of great interplate earthquakes. Besides great interplate events, however, many large (M>7) interplate, intraslab, outer-rise and tsunami earthquakes have also occurred in this region. First, we depicted the space-time pattern of M>7 earthquakes along the northern Japan to Kuril trench, based on the relocated mainshock-aftershock distributions of all types of earthquakes occurred since 1913. We relocated the hypocenters reported in the ISC, ISS, and BCIS bulletins by using the HYPOSAT (Schweitzer, 2003) and the Modified JHD method (Hurukawa, 1995). Then, in order to examine more detail space pattern, or rupture areas, of M>7 earthquakes since 1963, we estimated coseismic slip distributions by the Kikuchi and Kanamori's (2003) teleseismic body wave inversion method. The WWSSN waveform data were used for earthquakes between 1963 and 1990, and digital teleseismic waveform data compiled by the IRIS were used for events after 1990. Mainshock hypocenters relocated by us were used as initial rupture points. As a result, we found that the more complex feature of earthquake occurrence. Each region has been ruptured by a M8-class interplate earthquake or by multiple M7-class events. Offshore Urup Is. is source region of the 1963 Urup earthquake (M 8.5). Large interplate earthquakes occurred in the eastern and western part of the 1963 source region in 1991 (M 7.6) and 1995 (M 7.9), respectively. Their aftershock areas almost re-occupied the 1963 aftershock area. The 1963, 1991, and 1995 coseismic slip distributions show that the southwestern

  16. Module Configuration

    DOEpatents

    Oweis, Salah; D'Ussel, Louis; Chagnon, Guy; Zuhowski, Michael; Sack, Tim; Laucournet, Gaullume; Jackson, Edward J.

    2002-06-04

    A stand alone battery module including: (a) a mechanical configuration; (b) a thermal management configuration; (c) an electrical connection configuration; and (d) an electronics configuration. Such a module is fully interchangeable in a battery pack assembly, mechanically, from the thermal management point of view, and electrically. With the same hardware, the module can accommodate different cell sizes and, therefore, can easily have different capacities. The module structure is designed to accommodate the electronics monitoring, protection, and printed wiring assembly boards (PWAs), as well as to allow airflow through the module. A plurality of modules may easily be connected together to form a battery pack. The parts of the module are designed to facilitate their manufacture and assembly.

  17. Aeromonas spp. simultaneously harbouring bla(CTX-M-15), bla(SHV-12), bla(PER-1) and bla(FOX-2), in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia.

    PubMed

    Maravić, Ana; Skočibušić, Mirjana; Samanić, Ivica; Fredotović, Zeljana; Cvjetan, Svjetlana; Jutronić, Marinka; Puizina, Jasna

    2013-09-01

    Aeromonas species are becoming renowned as emerging pathogens by increasingly giving rise to a wide spectrum of food and waterborne infections in humans. Another worrisome feature of aeromonads is the growing frequency of antibiotic resistance as a consequence of their prominent diversity in terms of resistance determinants. This study aimed at determining the antimicrobial resistance pattern, prevalence and characterization of acquired β-lactamases, including extended-spectrum-β-lactamases (ESBLs) and AmpC cephalosporinases, as well as the presence of class 1 and 2 integrons, in Aeromonas isolates from wild-growing Mediterranean mussel (Mytilus galloprovincialis) of the eastern coast of Adriatic Sea, Croatia. Isolates were tested for susceptibility to 16 antibiotics and β-lactam/β-lactamase inhibitor combinations. Cephalosporin-resistant isolates were further screened by PCR for genes encoding AmpC (bla(FOX), bla(CMY), bla(MOX), bla(LAT), bla(BIL), bla(DHA), bla(ACC), bla(MIR), bla(ACT)), ESBLs (bla(TEM), bla(SHV), bla(CTX-M), bla(PER), bla(VEB), bla(GES/IBC), bla(OXA)) and integrases (intI1, intI2, intI3). Location of bla genes was characterized by plasmid DNA fingerprinting and Southern blot hybridization. Plasmids carrying ESBL genes were investigated for transferability by conjugation and PCR-based replicon typed. Out of 147 Aeromonas isolates recovered, 30 (20%) demonstrated multiple resistance profile, with co-resistance most frequently detected against penicillins, piperacillin/sulbactam and tetracycline. ESBL-encoding genes were detected in 21 (13 Aeromonas caviae and 8 Aeromonas hydrophila) isolates, with bla(CTX-M-15) gene identified in 19 and bla(SHV-12) in 12 isolates. Among them, 10 isolates simultaneously harboured bla(CTX-M-15) and bla(SHV-12), while 3 isolates additionally carried an AmpC β-lactamase bla(FOX-2) gene. bla(PER-1) gene was identified in a single isolate also harbouring the bla(CTX-M-15) gene. While bla(SHV-12) was chromosomally

  18. Aeromonas spp. simultaneously harbouring bla(CTX-M-15), bla(SHV-12), bla(PER-1) and bla(FOX-2), in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia.

    PubMed

    Maravić, Ana; Skočibušić, Mirjana; Samanić, Ivica; Fredotović, Zeljana; Cvjetan, Svjetlana; Jutronić, Marinka; Puizina, Jasna

    2013-09-01

    Aeromonas species are becoming renowned as emerging pathogens by increasingly giving rise to a wide spectrum of food and waterborne infections in humans. Another worrisome feature of aeromonads is the growing frequency of antibiotic resistance as a consequence of their prominent diversity in terms of resistance determinants. This study aimed at determining the antimicrobial resistance pattern, prevalence and characterization of acquired β-lactamases, including extended-spectrum-β-lactamases (ESBLs) and AmpC cephalosporinases, as well as the presence of class 1 and 2 integrons, in Aeromonas isolates from wild-growing Mediterranean mussel (Mytilus galloprovincialis) of the eastern coast of Adriatic Sea, Croatia. Isolates were tested for susceptibility to 16 antibiotics and β-lactam/β-lactamase inhibitor combinations. Cephalosporin-resistant isolates were further screened by PCR for genes encoding AmpC (bla(FOX), bla(CMY), bla(MOX), bla(LAT), bla(BIL), bla(DHA), bla(ACC), bla(MIR), bla(ACT)), ESBLs (bla(TEM), bla(SHV), bla(CTX-M), bla(PER), bla(VEB), bla(GES/IBC), bla(OXA)) and integrases (intI1, intI2, intI3). Location of bla genes was characterized by plasmid DNA fingerprinting and Southern blot hybridization. Plasmids carrying ESBL genes were investigated for transferability by conjugation and PCR-based replicon typed. Out of 147 Aeromonas isolates recovered, 30 (20%) demonstrated multiple resistance profile, with co-resistance most frequently detected against penicillins, piperacillin/sulbactam and tetracycline. ESBL-encoding genes were detected in 21 (13 Aeromonas caviae and 8 Aeromonas hydrophila) isolates, with bla(CTX-M-15) gene identified in 19 and bla(SHV-12) in 12 isolates. Among them, 10 isolates simultaneously harboured bla(CTX-M-15) and bla(SHV-12), while 3 isolates additionally carried an AmpC β-lactamase bla(FOX-2) gene. bla(PER-1) gene was identified in a single isolate also harbouring the bla(CTX-M-15) gene. While bla(SHV-12) was chromosomally

  19. Complete Nucleotide Sequence of a 92-Kilobase Plasmid Harboring the CTX-M-15 Extended-Spectrum Beta-Lactamase Involved in an Outbreak in Long-Term-Care Facilities in Toronto, Canada

    PubMed Central

    Boyd, David A.; Tyler, Shaun; Christianson, Sara; McGeer, Allison; Muller, Matthew P.; Willey, Barbara M.; Bryce, Elizabeth; Gardam, Michael; Nordmann, Patrice; Mulvey, Michael R.

    2004-01-01

    A major outbreak involving an Escherichia coli strain that was resistant to expanded-spectrum cephalosporins occurred in Toronto and surrounding regions in 2000 to 2002. We report the complete sequence of a plasmid, pC15-1a, that was found associated with the outbreak strain. Plasmid pC15-1a is a circular molecule of 92,353 bp consisting of two distinct regions. The first is a 64-kb region that is essentially homologous to the non-R-determinant region of plasmid R100 except for several point mutations, a few small insertions and deletions, and the absence of Tn10. The second is a 28.4-kb multidrug resistance region (MDR) that has replaced the R-determinant region of the R100 progenitor and consists mostly of transposons or partial transposons and five copies of the insertion element IS26. All drug resistance genes found in pC15-1a, including the beta-lactamase genes blaCTX-M-15, blaOXA-1, and blaTEM-1, the tetracycline resistance gene tetA, and aminoglycoside resistance genes aac(6′)-Ib and aac(3)-II, are located in the MDR. The blaCTX-M-15 gene was found downstream of ISEcp1as part of a transposition unit, as determined from the surrounding sequence. Examination of the plasmids from CTX-M-15-harboring strains isolated from hospitals across Canada showed that pC15-1a was found in several strains isolated from a site in western Canada. Comparison of pC15-1a and pCTX15, found in an E. coli strain isolated in India in 1999, revealed that the plasmids had several features in common, including an R100 backbone and several of the resistance genes, including blaCTX-M-15, blaTEM-1, blaOXA-1, tetA, and aac(6′)-Ib. PMID:15388431

  20. CTX-M-15-non-ST131 Escherichia coli isolates are mainly responsible of faecal carriage with ESBL-producing Enterobacteriaceae in travellers, immigrants and those visiting friends and relatives.

    PubMed

    Valverde, A; Turrientes, M-C; Norman, F; San Martín, E; Moreno, L; Pérez-Molina, J A; López-Vélez, R; Cantón, R

    2015-03-01

    Prevalence of extended-spectrum β-lactamases (ESBL) and/or carbapenemase-producing Enterobacteriaceae (EPE and CPE) in stool samples from 75 travellers, 8 people visiting friends and relatives and 3 immigrants who had travelled or came from tropical or subtropical areas was determined. Thirty-one per cent (27/86) of the subjects were faecal carriers of EPE, and 37 EPE isolates were recovered (36 Escherichia coli, 1 Klebsiella pneumoniae). CTX-M-15 was the most prevalent enzyme (64.8%) mainly associated with E. coli belonging to phylogroup A and sequence type complex 10. Most of the ESBL-positive travellers (50%) had visited countries from Asia.

  1. Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.

    PubMed

    Devarkar, Swapnil C; Wang, Chen; Miller, Matthew T; Ramanathan, Anand; Jiang, Fuguo; Khan, Abdul G; Patel, Smita S; Marcotrigiano, Joseph

    2016-01-19

    RNAs with 5'-triphosphate (ppp) are detected in the cytoplasm principally by the innate immune receptor Retinoic Acid Inducible Gene-I (RIG-I), whose activation triggers a Type I IFN response. It is thought that self RNAs like mRNAs are not recognized by RIG-I because 5'ppp is capped by the addition of a 7-methyl guanosine (m7G) (Cap-0) and a 2'-O-methyl (2'-OMe) group to the 5'-end nucleotide ribose (Cap-1). Here we provide structural and mechanistic basis for exact roles of capping and 2'-O-methylation in evading RIG-I recognition. Surprisingly, Cap-0 and 5'ppp double-stranded (ds) RNAs bind to RIG-I with nearly identical Kd values and activate RIG-I's ATPase and cellular signaling response to similar extents. On the other hand, Cap-0 and 5'ppp single-stranded RNAs did not bind RIG-I and are signaling inactive. Three crystal structures of RIG-I complexes with dsRNAs bearing 5'OH, 5'ppp, and Cap-0 show that RIG-I can accommodate the m7G cap in a cavity created through conformational changes in the helicase-motif IVa without perturbing the ppp interactions. In contrast, Cap-1 modifications abrogate RIG-I signaling through a mechanism involving the H830 residue, which we show is crucial for discriminating between Cap-0 and Cap-1 RNAs. Furthermore, m7G capping works synergistically with 2'-O-methylation to weaken RNA affinity by 200-fold and lower ATPase activity. Interestingly, a single H830A mutation restores both high-affinity binding and signaling activity with 2'-O-methylated dsRNAs. Our work provides new structural insights into the mechanisms of host and viral immune evasion from RIG-I, explaining the complexity of cap structures over evolution. PMID:26733676

  2. Desorption Kinetics of H2O from Cab-O-Sil-M-7D and Hi-Sil-233 Silica Particles

    SciTech Connect

    Dinh, L.; Balooch, M.; LeMay, J.D.

    2000-01-26

    Temperature programmed desorption (TPD) was performed at temperatures up to 850K on Cab-O-Sil-M-7D and Hi-Sil-233 silica particles. Physisorbed water molecules on both types of silica had activation energies in the range of 9-14.5 kcal/mol. However, the activation energies of desorption for chemisorbed water varied from {approx} 19 kcal/mol to > 59 kcal/mol for Cab-O-Sil-M-7D, and {approx} 23-37 kcal/mol for Hi-Sil-233. Our results suggest that physisorbed water can be effectively pumped away at room temperature (or preferably at 320 K) in a matter of hours. Chemisorbed water with high activation energies of desorption (>30 kcal/mol) will not escape the silica surfaces in 100 years even at 320 K, while a significant amount of the chemisorbed water with medium activation energies (19-26 kcal/mol) will leave the silica surfaces in that time span. Most of the chemisorbed water with activation energies < 30 kcal/mol can be pumped away in a matter of days in a good vacuum environment at 500 K. We had previously measured about 0.1-0.4 wt. % of water in M9787 polysiloxane formulations containing {approx} 21% Cab-O-Sil-M-7D and {approx} 4% Hi-Sil-233. Comparing present results with these formulations, we conclude that absorbed H{sub 2}O and Si-OH bonds on the silica surfaces are the major contributors to water outgassing from M97 series silicones.

  3. Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium.

    PubMed

    Whittington, Richard J; Whittington, Ann-Michele; Waldron, Anna; Begg, Douglas J; de Silva, Kumi; Purdie, Auriol C; Plain, Karren M

    2013-12-01

    Liquid culture of Mycobacterium avium subsp. paratuberculosis from clinical samples, such as feces, is the most sensitive antemortem test for the diagnosis of Johne's disease in ruminants. In Australia, New Zealand, the United States, and some other countries, the Bactec 460 system with modified Bactec 12B medium (Becton, Dickinson) has been the most commonly used liquid culture system, but it was discontinued in 2012. In this study, a new liquid culture medium, M7H9C, was developed. It consists of a Middlebrook 7H9 medium base with added Casitone, albumin, dextrose, catalase, egg yolk, mycobactin J, and a cocktail of antibiotics. We found that polyoxyethylene stearate (POES) was not essential for the cultivation of M. avium subsp. paratuberculosis in either the Bactec 12B or the M7H9C medium. The limit of detection determined using pure cultures of the C and S strains of M. avium subsp. paratuberculosis was 7 bacilli per 50 μl inoculum in the two media. The new medium was validated using 784 fecal and tissue samples from sheep and cattle, >25% of which contained viable M. avium subsp. paratuberculosis. Discrepant results for the clinical samples between the two media were mostly associated with samples that contained <10 viable bacilli per gram, but these results were relatively uncommon, and the performances of the two media were not significantly different. M7H9C medium was less than half the cost of the Bactec 12B medium and did not require regular examination during incubation, but a confirmatory IS900 PCR test had to be performed on every culture after the predetermined incubation period.

  4. Structural Insight into Potent Broad-Spectrum Inhibition with Reversible Recyclization Mechanism: Avibactam in Complex with CTX-M-15 and Pseudomonas aeruginosa AmpC β-Lactamases

    PubMed Central

    Mangani, Stefano; Durand-Reville, Thomas; Benvenuti, Manuela; De Luca, Filomena; Sanyal, Gautam

    2013-01-01

    Although β-lactams have been the most effective class of antibacterial agents used in clinical practice for the past half century, their effectiveness on Gram-negative bacteria has been eroded due to the emergence and spread of β-lactamase enzymes that are not affected by currently marketed β-lactam/β-lactamase inhibitor combinations. Avibactam is a novel, covalent, non-β-lactam β-lactamase inhibitor presently in clinical development in combination with either ceftaroline or ceftazidime. In vitro studies show that avibactam may restore the broad-spectrum activity of cephalosporins against class A, class C, and some class D β-lactamases. Here we describe the structures of two clinically important β-lactamase enzymes bound to avibactam, the class A CTX-M-15 extended-spectrum β-lactamase and the class C Pseudomonas aeruginosa AmpC β-lactamase, which together provide insight into the binding modes for the respective enzyme classes. The structures reveal similar binding modes in both enzymes and thus provide a rationale for the broad-spectrum inhibitory activity of avibactam. Identification of the key residues surrounding the binding pocket allows for a better understanding of the potency of this scaffold. Finally, avibactam has recently been shown to be a reversible inhibitor, and the structures provide insights into the mechanism of avibactam recyclization. Analysis of the ultra-high-resolution CTX-M-15 structure suggests how the deacylation mechanism favors recyclization over hydrolysis. PMID:23439634

  5. [Distribution of phylogenetic groups and virulence factors in CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli strains isolated from patients in the community of Mérida, Venezuela].

    PubMed

    Millán, Ysheth; Hernández, Erick; Millán, Beatriz; Araque, María

    2014-01-01

    In this study, the distribution of phylogenetic groups and the genetic detection of virulence factors in CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli (UPEC) strains were analyzed. Twenty eight strains were isolated between January 2009 and July 2011 from patients with urinary tract infection (UTI) who attended the Public Health Laboratory at Mérida, Venezuela. Determination of phylogenetic groups and detection of six virulence genes, fimH, fyuA, kpsMTII, usp, PAI and papAH, were performed by PCR amplification. Fifteen of the 28 isolates were mainly located in the phylogenetic group A, followed by B2 (12/28) and D (1/28). No direct relationship between the severity or recurrence of UTI and the distribution of phylogroups was observed. All studied virulence factors were found in group B2 strains with the highest frequency. The prevalent virulence profile included the combination of three main genes: fimH, kpsMTII and fyuA and, to a lesser extent, the presence of other determinants such as usp, PAI and/or papAH. These results indicate that virulent UPEC incorporated three important properties: adhesion, iron uptake and evasion of phagocytosis, which favored the production of recurrent UTI. This is the first report describing the association of phylogenetic groups with the potential virulence of CTX-M-15 β-lactamase producing UPEC strains in Venezuela.

  6. 12 May 2008 M = 7.9 Wenchuan, China, earthquake calculated to increase failure stress and seismicity rate on three major fault systems

    USGS Publications Warehouse

    Toda, S.; Lin, J.; Meghraoui, M.; Stein, R.S.

    2008-01-01

    The Wenchuan earthquake on the Longmen Shan fault zone devastated cities of Sichuan, claiming at least 69,000 lives. We calculate that the earthquake also brought the Xianshuihe, Kunlun and Min Jiang faults 150-400 km from the mainshock rupture in the eastern Tibetan Plateau 0.2-0.5 bars closer to Coulomb failure. Because some portions of these stressed faults have not ruptured in more than a century, the earthquake could trigger or hasten additional M > 7 earthquakes, potentially subjecting regions from Kangding to Daofu and Maqin to Rangtag to strong shaking. We use the calculated stress changes and the observed background seismicity to forecast the rate and distribution of damaging shocks. The earthquake probability in the region is estimated to be 57-71% for M ??? 6 shocks during the next decade, and 8-12% for M ??? 7 shocks. These are up to twice the probabilities for the decade before the Wenchuan earthquake struck. Copyright 2008 by the American Geophysical Union.

  7. Strong-motion observations of the M 7.8 Gorkha, Nepal, earthquake sequence and development of the N-shake strong-motion network

    USGS Publications Warehouse

    Dixit, Amod; Ringler, Adam; Sumy, Danielle F.; Cochran, Elizabeth S.; Hough, Susan E.; Martin, Stacey; Gibbons, Steven; Luetgert, James H.; Galetzka, John; Shrestha, Surya; Rajaure, Sudhir; McNamara, Daniel E.

    2015-01-01

    We present and describe strong-motion data observations from the 2015 M 7.8 Gorkha, Nepal, earthquake sequence collected using existing and new Quake-Catcher Network (QCN) and U.S. Geological Survey NetQuakes sensors located in the Kathmandu Valley. A comparison of QCN data with waveforms recorded by a conventional strong-motion (NetQuakes) instrument validates the QCN data. We present preliminary analysis of spectral accelerations, and peak ground acceleration and velocity for earthquakes up to M 7.3 from the QCN stations, as well as preliminary analysis of the mainshock recording from the NetQuakes station. We show that mainshock peak accelerations were lower than expected and conclude the Kathmandu Valley experienced a pervasively nonlinear response during the mainshock. Phase picks from the QCN and NetQuakes data are also used to improve aftershock locations. This study confirms the utility of QCN instruments to contribute to ground-motion investigations and aftershock response in regions where conventional instrumentation and open-access seismic data are limited. Initial pilot installations of QCN instruments in 2014 are now being expanded to create the Nepal–Shaking Hazard Assessment for Kathmandu and its Environment (N-SHAKE) network.

  8. Fault-Zone Trapped Waves from Aftershocks of the M7.2 Darfield and M6.3 Christchurch Earthquake Sequence for Document of Subsurface Damage Zones

    NASA Astrophysics Data System (ADS)

    Li, Y.; De Pascale, G. P.; Gravley, D.; Cherrington, J.; Alvarez, M. G.

    2011-12-01

    The M6.3 Christchurch earthquake struck the Canterbury region in NZ's South Island on 22 February 2011, following ~6 months after the Sept. 4, 2010 M7.1 Darfield earthquake in the same region. It has generated a significant series of aftershocks, many of which are considered big for a M6.3 earthquake. It is not know clearly whether the later M6.3 event is technically an aftershock because of its relationship to the ongoing activity since September last year, or it is a separate event, given its location on a separate fault system, a previously unknown blind fault line running 17 km south of Christchurch. In order to study the complicated subsurface structure of the damage zones caused by this sequence of earthquakes in NZ, under the support of NSF-RAPID Program, we deployed 12 PASSCAL seismographs in two ~300-m long seismic lines across the Greendale fault where the horizontal right-lateral slip of 4.5 m and vertical slip of 1.6 m were caused by the 2010 M7.2 Darfield earthquake and the aftershock zone of the M6.3 Christchurch earthquake, respectively, to record fault-zone trapped waves (FZTWs) generated by aftershocks, starting from May 5th, 2011. We have recorded the data for ~300 M>3 aftershocks with good locations and more than ~1000 small events not located yet but with good signal-to-noise ratio at these two arrays, including M5.3, M6, M5.4, M5.1 aftershocks with their clustered events at depths of 10-15 km. Preliminary examination of the waveform data shows FZTWs clearly at stations located within the 50-75-m wide rupture zone with high density of en-echelon cracks on the ground surface along the Greendale fault. 3-D finite-difference simulations of these FZTWs show a distinct low-velocity zone (LVZ) at seismogenic depth, indicating that the Greendale fault has undergone strong dynamic stresses and pervasive cracking during the 2010 M7.2 Darfield earthquake. We interpret this LVZ as being a remnant of damage zone in dynamic ruptures that accumulated damage

  9. Multiresistant Uropathogenic Escherichia coli from a Region in India Where Urinary Tract Infections Are Endemic: Genotypic and Phenotypic Characteristics of Sequence Type 131 Isolates of the CTX-M-15 Extended-Spectrum-β-Lactamase-Producing Lineage

    PubMed Central

    Hussain, Arif; Ewers, Christa; Nandanwar, Nishant; Guenther, Sebastian; Jadhav, Savita; Wieler, Lothar H.

    2012-01-01

    Escherichia coli sequence type 131 (O25b:H4), associated with the CTX-M-15 extended-spectrum beta-lactamases (ESBLs) and linked predominantly to the community-onset antimicrobial-resistant infections, has globally emerged as a public health concern. However, scant attention is given to the understanding of the molecular epidemiology of these strains in high-burden countries such as India. Of the 100 clinical E. coli isolates obtained by us from a setting where urinary tract infections are endemic, 16 ST131 E. coli isolates were identified by multilocus sequence typing (MLST). Further, genotyping and phenotyping methods were employed to characterize their virulence and drug resistance patterns. All the 16 ST131 isolates harbored the CTX-M-15 gene, and half of them also carried TEM-1; 11 of these were positive for blaOXA groups 1 and 12 for aac(6′)-Ib-cr. At least 12 isolates were refractory to four non-beta-lactam antibiotics: ciprofloxacin, gentamicin, sulfamethoxazole-trimethoprim, and tetracycline. Nine isolates carried the class 1 integron. Plasmid analysis indicated a large pool of up to six plasmids per strain with a mean of approximately three plasmids. Conjugation and PCR-based replicon typing (PBRT) revealed that the spread of resistance was associated with the FIA incompatibility group of plasmids. Pulsed-field gel electrophoresis (PFGE) and genotyping of the virulence genes showed a low level of diversity among these strains. The association of ESBL-encoding plasmid with virulence was demonstrated in transconjugants by serum assay. None of the 16 ST131 ESBL-producing E. coli strains were known to synthesize carbapenemase enzymes. In conclusion, our study reports a snapshot of the highly virulent/multiresistant clone ST131 of uropathogenic E. coli from India. This study suggests that the ST131 genotypes from this region are clonally evolved and are strongly associated with the CTX-M-15 enzyme, carry a high antibiotic resistance background, and have

  10. Global study of great (M>= 7) deep focus seismic events having regard to the May 24, 2013 Mw 8.3 earthquake the Sea of Okhotsk, Russia

    NASA Astrophysics Data System (ADS)

    Varga, Peter; Rogozhin, Evgeny; Süle, Bálint; Andreeva, Nadezda

    2014-05-01

    Distribution of great seismic events M >= 7.0 and consequently the released seismic energy along the Earth radius is of bimodal character. 90% of the great seismic events, which are responsible for the most of energy released, occur relatively close to the Earth's surface, at an average depth of 50 km. The vast majority of remaining 10% is associated with seismic events that occur very deep, an average of 580-590 km, above the border between transition zone and lower mantle (660 km). These very deep earthquakes (depth >= 500 km) differ significantly from the shallow events. For the study of the distribution of M >= 7.0 earthquakes and their radiated energy a catalogue was completed for the time-interval between 1900 and 2013. Examination of the source zones in which both shallow and deep M >= 7.0 earthquakes occur shows that linear distribution of deep earthquakes is considerably shorter than that found for the shallow earthquakes, which determine the length of the zone. The position of very deep (≥ 500 km) earthquakes foci show where the down going lithosperic plates conflict with the upper boundary of lower mantle, and where they probably cross it. This passage generates compression - elongation inside the slab. A comparison of temporal distribution of shallow and deep seismic events of a given source zone suggests that there is no direct relationship in the distribution of these two different earthquake activities. The largest of these great deep earthquakes, the May 24, 2013 Mw 8.3 earthquake the Sea of Okhotsk, was preceded by an earthquake swarm, which consists 58 M >= 5 events and occurred between May 15 and 24, 2013 in the higher part of the sinking slab east of Kamchatka. The aftershock activity after the Okhotsk Sea earthquake was moderate: twelve events with magnitudes above M 4 were observed till June 27. These events determine a fault area (2.64x104 km2) similar to the case of a shallow M 8.3 event. The effect of Okhotsk Sea was felt throughout

  11. The C-terminal region of thermophilic tRNA (m7G46) methyltransferase (TrmB) stabilizes the dimer structure and enhances fidelity of methylation.

    PubMed

    Tomikawa, Chie; Ochi, Anna; Hori, Hiroyuki

    2008-05-15

    Transfer RNA (m(7)G46) methyltransferase catalyzes methyl-transfer from S-adenosyl-L-methionine to N(7) atom of the semi-conserved G46 base in tRNA. Aquifex aeolicus is a hyper thermophilic eubacterium that grows at close to 95 degrees C. A. aeolicus tRNA (m(7)G46) methyltransferase [TrmB] has an elongated C-terminal region as compared with mesophilic counterparts. In this study, the authors focused on the functions of this C-terminal region. Analytic gel filtration chromatography and amino acid sequencing reveled that the start point (Glu202) of the C-terminal region is often cleaved by proteases during purification steps and the C-terminal region tightly binds to another subunit even in the presence of 6M urea. Because the C-terminal region contains abundant basic amino acid residues, the authors assumed that some of these residues might be involved in tRNA binding. To address this idea, the authors prepared eight alanine substitution mutant proteins. However, measurements of initial velocities of these mutant proteins suggested that the basic amino acid residues in the C-terminal region are not involved in tRNA binding. The authors investigated effects of the deletion of the C-terminal region. Deletion mutant protein of the C-terminal region (the core protein) was precipitated by incubation at 85 degrees C, while the wild type protein was soluble at that temperature, demonstrating that the C-terminal region contributes to the protein stability at high temperatures. The core protein had a methyl-transfer activity to yeast tRNA(Phe) transcript. Furthermore, the core protein slowly methylated tRNA transcripts, which did not contain G46 base. Moreover, the modified base was identified as m(7)G by two-dimensional thin layer chromatography. Thus, the deletion of the C-terminal region causes nonspecific methylation of N(7) atom of guanine base(s) in tRNA transcripts.

  12. Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

    PubMed Central

    Devarkar, Swapnil C.; Wang, Chen; Miller, Matthew T.; Ramanathan, Anand; Jiang, Fuguo; Khan, Abdul G.; Patel, Smita S.; Marcotrigiano, Joseph

    2016-01-01

    RNAs with 5′-triphosphate (ppp) are detected in the cytoplasm principally by the innate immune receptor Retinoic Acid Inducible Gene-I (RIG-I), whose activation triggers a Type I IFN response. It is thought that self RNAs like mRNAs are not recognized by RIG-I because 5′ppp is capped by the addition of a 7-methyl guanosine (m7G) (Cap-0) and a 2′-O-methyl (2′-OMe) group to the 5′-end nucleotide ribose (Cap-1). Here we provide structural and mechanistic basis for exact roles of capping and 2′-O-methylation in evading RIG-I recognition. Surprisingly, Cap-0 and 5′ppp double-stranded (ds) RNAs bind to RIG-I with nearly identical Kd values and activate RIG-I’s ATPase and cellular signaling response to similar extents. On the other hand, Cap-0 and 5′ppp single-stranded RNAs did not bind RIG-I and are signaling inactive. Three crystal structures of RIG-I complexes with dsRNAs bearing 5′OH, 5′ppp, and Cap-0 show that RIG-I can accommodate the m7G cap in a cavity created through conformational changes in the helicase-motif IVa without perturbing the ppp interactions. In contrast, Cap-1 modifications abrogate RIG-I signaling through a mechanism involving the H830 residue, which we show is crucial for discriminating between Cap-0 and Cap-1 RNAs. Furthermore, m7G capping works synergistically with 2′-O-methylation to weaken RNA affinity by 200-fold and lower ATPase activity. Interestingly, a single H830A mutation restores both high-affinity binding and signaling activity with 2′-O-methylated dsRNAs. Our work provides new structural insights into the mechanisms of host and viral immune evasion from RIG-I, explaining the complexity of cap structures over evolution. PMID:26733676

  13. Rupture process of the M 7.9 Denali fault, Alaska, earthquake: Subevents, directivity, and scaling of high-frequency ground motions

    USGS Publications Warehouse

    Frankel, A.

    2004-01-01

    Displacement waveforms and high-frequency acceleration envelopes from stations at distances of 3-300 km were inverted to determine the source process of the M 7.9 Denali fault earthquake. Fitting the initial portion of the displacement waveforms indicates that the earthquake started with an oblique thrust subevent (subevent # 1) with an east-west-striking, north-dipping nodal plane consistent with the observed surface rupture on the Susitna Glacier fault. Inversion of the remainder of the waveforms (0.02-0.5 Hz) for moment release along the Denali and Totschunda faults shows that rupture proceeded eastward on the Denali fault, with two strike-slip subevents (numbers 2 and 3) centered about 90 and 210 km east of the hypocenter. Subevent 2 was located across from the station at PS 10 (Trans-Alaska Pipeline Pump Station #10) and was very localized in space and time. Subevent 3 extended from 160 to 230 km east of the hypocenter and had the largest moment of the subevents. Based on the timing between subevent 2 and the east end of subevent 3, an average rupture velocity of 3.5 km/sec, close to the shear wave velocity at the average rupture depth, was found. However, the portion of the rupture 130-220 km east of the epicenter appears to have an effective rupture velocity of about 5.0 km/ sec, which is supershear. These two subevents correspond approximately to areas of large surface offsets observed after the earthquake. Using waveforms of the M 6.7 Nenana Mountain earthquake as empirical Green's functions, the high-frequency (1-10 Hz) envelopes of the M 7.9 earthquake were inverted to determine the location of high-frequency energy release along the faults. The initial thrust subevent produced the largest high-frequency energy release per unit fault length. The high-frequency envelopes and acceleration spectra (>0.5 Hz) of the M 7.9 earthquake can be simulated by chaining together rupture zones of the M 6.7 earthquake over distances from 30 to 180 km east of the

  14. Genetic background of novel sequence types of CTX-M-8- and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae from public wastewater treatment plants in São Paulo, Brazil.

    PubMed

    Dropa, Milena; Lincopan, Nilton; Balsalobre, Livia C; Oliveira, Danielle E; Moura, Rodrigo A; Fernandes, Miriam Rodriguez; da Silva, Quézia Moura; Matté, Glavur R; Sato, Maria I Z; Matté, Maria H

    2016-03-01

    The release of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae to the environment is a public health issue worldwide. The aim of this study was to investigate the genetic background of genes encoding ESBLs in wastewater treatment plants (WWTPs) in São Paulo, southeastern Brazil. In 2009, during a local surveillance study, seven ESBL-producing Enterobacteriaceae strains were recovered from five WWTPs and screened for ESBL genes and mobile genetic elements. Multilocus sequence typing (MLST) was carried out, and wild plasmids were transformed into electrocompetent Escherichia coli. S1-PFGE technique was used to verify the presence of high molecular weight plasmids in wild-type strains and in bla ESBL-containing E. coli transformants. Strains harbored bla CTX-M-8, bla CTX-M-15, and/or bla SHV-28. Sequencing results showed that bla CTX-M-8 and bla CTX-M-15 genes were associated with IS26. MLST revealed new sequence types for E. coli (ST4401, ST4402, ST4403, and ST4445) and Klebsiella pneumoniae (ST1574), except for one K. pneumoniae from ST307 and Enterobacter cloacae from ST131. PCR and S1-PFGE results showed CTX-M-producing E. coli transformants carried heavy plasmids sizing 48.5-209 kb, which belonged to IncI1, IncF, and IncM1 incompatibility groups. This is the first report of CTX-M-8 and SHV-28 enzymes in environmental samples, and the present results demonstrate the plasmid-mediated spread of CTX-M-encoding genes through five WWTPs in São Paulo, Brazil, suggesting WWTPs are hotspots for the transfer of ESBL genes and confirming the urgent need to improve the management of sewage in order to minimize the dissemination of resistance genes to the environment. PMID:26782324

  15. Genetic background of novel sequence types of CTX-M-8- and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae from public wastewater treatment plants in São Paulo, Brazil.

    PubMed

    Dropa, Milena; Lincopan, Nilton; Balsalobre, Livia C; Oliveira, Danielle E; Moura, Rodrigo A; Fernandes, Miriam Rodriguez; da Silva, Quézia Moura; Matté, Glavur R; Sato, Maria I Z; Matté, Maria H

    2016-03-01

    The release of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae to the environment is a public health issue worldwide. The aim of this study was to investigate the genetic background of genes encoding ESBLs in wastewater treatment plants (WWTPs) in São Paulo, southeastern Brazil. In 2009, during a local surveillance study, seven ESBL-producing Enterobacteriaceae strains were recovered from five WWTPs and screened for ESBL genes and mobile genetic elements. Multilocus sequence typing (MLST) was carried out, and wild plasmids were transformed into electrocompetent Escherichia coli. S1-PFGE technique was used to verify the presence of high molecular weight plasmids in wild-type strains and in bla ESBL-containing E. coli transformants. Strains harbored bla CTX-M-8, bla CTX-M-15, and/or bla SHV-28. Sequencing results showed that bla CTX-M-8 and bla CTX-M-15 genes were associated with IS26. MLST revealed new sequence types for E. coli (ST4401, ST4402, ST4403, and ST4445) and Klebsiella pneumoniae (ST1574), except for one K. pneumoniae from ST307 and Enterobacter cloacae from ST131. PCR and S1-PFGE results showed CTX-M-producing E. coli transformants carried heavy plasmids sizing 48.5-209 kb, which belonged to IncI1, IncF, and IncM1 incompatibility groups. This is the first report of CTX-M-8 and SHV-28 enzymes in environmental samples, and the present results demonstrate the plasmid-mediated spread of CTX-M-encoding genes through five WWTPs in São Paulo, Brazil, suggesting WWTPs are hotspots for the transfer of ESBL genes and confirming the urgent need to improve the management of sewage in order to minimize the dissemination of resistance genes to the environment.

  16. Phylogenetic Distribution and Prevalence of Genes Encoding Class I Integrons and CTX-M-15 Extended-Spectrum β-Lactamases in Escherichia coli Isolates from Healthy Humans in Chandigarh, India

    PubMed Central

    Dureja, Chetna; Mahajan, Sakshi; Raychaudhuri, Saumya

    2014-01-01

    Escherichia coli is generally considered as a commensal inhabitant of gastrointestinal tract of humans and animals. The aim of this study was to gain insight on the distribution of phylotypes and presence of genes encoding integrons, extended β-lactamases and resistance to other antimicrobials in the commensal E. coli isolates from healthy adults in Chandigarh, India. PCR and DNA sequencing were used for phylogenetic classification, detections of integrase genes, gene cassettes within the integron and extended β-lactamases. The genetic structure of E. coli revealed a non-uniform distribution of isolates among the seven phylogenetic groups with significant representation of group A. Integron-encoded integrases were detected in 25 isolates with class 1 integron-encoded intI1 integrase being in the majority (22 isolates). The gene cassettes identified were those for trimethoprim, streptomycin, spectinomycin and streptothricin. The dfrA12-orfF-aadA2 was the most commonly found gene cassette in intI1 positive isolates. Phenotypic assay for screening the potential ESBL producers suggested 16 isolates to be ESBL producers. PCR detection using gene-specific primers showed that 15 out of these 16 ESBL-producing E. coli harboured the blaCTX-M-15 gene. Furthermore, molecular studies helped in characterizing the genes responsible for tetracycline, chloramphenicol and sulphonamides resistance. Collectively, our study outlines the intra-species phylogenetic structure and highlights the prevalence of class 1 integron and blaCTX-M-15 in commensal E. coli isolates of healthy adults in Chandigarh, India. Our findings further reinforce the relevance of commensal E. coli strains on the growing burden of antimicrobial resistance. PMID:25409321

  17. Frequency of conjugative transfer of plasmid-encoded ISEcp1 - blaCTX-M-15 and aac(6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon - role of transferases

    PubMed Central

    2010-01-01

    Background The frequency of transfer of genes encoding resistance to antimicrobial agents was determined by conjugation in ESBL-producing and/or fluoroquinolone or aminoglycoside resistant Enterobacteriaceae clinical isolates at a tertiary care center in Lebanon. In addition, the role of tra genes encoding transferases in mediating conjugation was assessed. Methods Conjugation experiments were done on 53 ESBL-producing and/or fluoroquinolone resistant E. coli and K. pneumoniae and ESBL-producing S. sonnei isolates. Antimicrobial susceptibility testing on parent and transconjugant isolates, and PCR amplifications on plasmid extracts of the resistance-encoding genes: blaCTX-M-15 with the ISEcp1 insertion sequence, the aac(6')-lb-cr and qnrS genes, as well as tra encoding transferases genes were done. Random amplified polymorphic DNA (RAPD) analysis was performed to demonstrate whether conjugative isolates are clonal and whether they are linked epidemiologically to a particular source. Results Antimicrobial susceptibility testing on transconjugants revealed that 26 out of 53 (49%) ESBL-producing Enterobacteriaceae were able to transfer antimicrobial resistance to the recipients. Transfer of high-level resistance to the transconjugants encoded by the blaCTX-M-15 gene downstream the ISEcp1 insertion sequence against 3rd generation cephalosporins, and of low-level resistance against ciprofloxacin, and variable levels of resistance against aminoglycosides encoded by aac(6')-lb-cr gene, were observed in transconjugants. tra encoding transferase genes were detected exclusively in conjugative isolates. Conclusion In conclusion, the frequency of transfer of antimicrobial resistance in non clonal Enterobacteriaceae at the tertiary care center by conjugation was 49%. Conjugation occurred in isolates expressing the tra encoding transferase genes. Multiple conjugative strains harboring the plasmid encoded antimicrobial resistant genes were circulating in the medical center

  18. Real-Time Science on Social Media: The Example of Twitter in the Minutes, Hours, Days after the 2015 M7.8 Nepal Earthquake

    NASA Astrophysics Data System (ADS)

    Lomax, A.; Bossu, R.; Mazet-Roux, G.

    2015-12-01

    Scientific information on disasters such as earthquakes typically comes firstly from official organizations, news reports and interviews with experts, and later from scientific presentations and peer-reviewed articles. With the advent of the Internet and social media, this information is available in real-time from automated systems and within a dynamic, collaborative interaction between scientific experts, responders and the public. After the 2015 M7.8 Nepal earthquake, Twitter Tweets from earth scientists* included information, analysis, commentary and discussion on earthquake parameters (location, size, mechanism, rupture extent, high-frequency radiation, …), earthquake effects (distribution of felt shaking and damage, triggered seismicity, landslides, …), earthquake rumors (e.g. the imminence of a larger event) and other earthquake information and observations (aftershock forecasts, statistics and maps, source and regional tectonics, seismograms, GPS, InSAR, photos/videos, …).In the future (while taking into account security, false or erroneous information and identity verification), collaborative, real-time science on social media after a disaster will give earlier and better scientific understanding and dissemination of public information, and enable improved emergency response and disaster management.* A sample of scientific Tweets after the 2015 Nepal earthquake: In the first minutes: "mb5.9 Mwp7.4 earthquake Nepal 2015.04.25-06:11:25UTC", "Major earthquake shakes Nepal 8 min ago", "Epicenter between Pokhara and Kathmandu", "Major earthquake shakes Nepal 18 min ago. Effects derived from witnesses' reports". In the first hour: "shallow thrust faulting to North under Himalayas", "a very large and shallow event ... Mw7.6-7.7", "aftershocks extend east and south of Kathmandu, so likely ruptured beneath city", "Valley-blocking landslides must be a very real worry". In the first day: "M7.8 earthquake in Nepal 2hr ago: destructive in Kathmandu Valley and

  19. Multidrug resistance and high virulence genotype in uropathogenic Escherichia coli due to diffusion of ST131 clonal group producing CTX-M-15: an emerging problem in a Tunisian hospital.

    PubMed

    Ferjani, Sana; Saidani, Mabrouka; Ennigrou, Samir; Hsairi, Mohamed; Slim, Amine Faouzi; Ben Boubaker, Ilhem Boutiba

    2014-05-01

    A collection of 201 Escherichia coli strains isolated from urine of patients in a Tunisian hospital between January 2006 and July 2008 was studied. Microbial identification was done by conventional methods, and antibiotic susceptibility with disk diffusion method was performed according to the Clinical Laboratory and Standards Institute guidelines. Detection of extended-spectrum beta-lactamase (ESBL) was performed by double-disk synergy test (DDST) and identification was done by PCR and sequencing. ESBL-producing isolates were subjected to molecular typing by random amplified polymorphic DNA (RAPD) and ST131 detection by PCR. Four phylogenetic groups (A, B1, B2 and D), 18 virulence genes and CTX-M group were individualized using PCR. Statistical analysis was done by Pearson χ2 test and Mann-Whitney U test. The strains were recovered primarily from urology (28%), maternity (19%) and medicine (16%) wards. Antibiotic resistance rates were ampicilin (72.1%), nalidixic acid (41.8%), ciprofloxacin (38.8%), gentamicin (23.9%) and cefotaxime (17.4%). Thirty-one of cefotaxime-resistant isolates (n = 35) had a positive DDST and harboured bla CTX-M-15 gene. Twenty of them (64.5%) belonged to the ST131 clone and showed the same RAPD DNA profile. Ciprofloxacin- and cotrimoxazole-susceptible isolates were significantly associated with phylogenetic group B2, whereas isolates that were resistant to these molecules were associated with B1 and D phylogenetic groups, respectively. Virulence genes were significantly more frequent among ciprofloxacin- and cotrimoxazole-susceptible strains than those resistant to these antibiotics. However, CXT-M-15-producing isolates were associated with many virulence genes. Isolates concomitantly susceptible to the three antimicrobials agents (ciprofloxacin, cefotaxime and cotrimoxazole) were significantly associated with group B2 and high virulence score, whereas isolates with resistance patterns especially those including resistance to

  20. N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network

    PubMed Central

    Tomikawa, Chie; Yokogawa, Takashi; Kanai, Tamotsu; Hori, Hiroyuki

    2010-01-01

    N7-methylguanine at position 46 (m7G46) in tRNA is produced by tRNA (m7G46) methyltransferase (TrmB). To clarify the role of this modification, we made a trmB gene disruptant (ΔtrmB) of Thermus thermophilus, an extreme thermophilic eubacterium. The absence of TrmB activity in cell extract from the ΔtrmB strain and the lack of the m7G46 modification in tRNAPhe were confirmed by enzyme assay, nucleoside analysis and RNA sequencing. When the ΔtrmB strain was cultured at high temperatures, several modified nucleotides in tRNA were hypo-modified in addition to the lack of the m7G46 modification. Assays with tRNA modification enzymes revealed hypo-modifications of Gm18 and m1G37, suggesting that the m7G46 positively affects their formations. Although the lack of the m7G46 modification and the hypo-modifications do not affect the Phe charging activity of tRNAPhe, they cause a decrease in melting temperature of class I tRNA and degradation of tRNAPhe and tRNAIle. 35S-Met incorporation into proteins revealed that protein synthesis in ΔtrmB cells is depressed above 70°C. At 80°C, the ΔtrmB strain exhibits a severe growth defect. Thus, the m7G46 modification is required for cell viability at high temperatures via a tRNA modification network, in which the m7G46 modification supports introduction of other modifications. PMID:19934251

  1. N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network.

    PubMed

    Tomikawa, Chie; Yokogawa, Takashi; Kanai, Tamotsu; Hori, Hiroyuki

    2010-01-01

    N(7)-methylguanine at position 46 (m(7)G46) in tRNA is produced by tRNA (m(7)G46) methyltransferase (TrmB). To clarify the role of this modification, we made a trmB gene disruptant (DeltatrmB) of Thermus thermophilus, an extreme thermophilic eubacterium. The absence of TrmB activity in cell extract from the DeltatrmB strain and the lack of the m(7)G46 modification in tRNA(Phe) were confirmed by enzyme assay, nucleoside analysis and RNA sequencing. When the DeltatrmB strain was cultured at high temperatures, several modified nucleotides in tRNA were hypo-modified in addition to the lack of the m(7)G46 modification. Assays with tRNA modification enzymes revealed hypo-modifications of Gm18 and m(1)G37, suggesting that the m(7)G46 positively affects their formations. Although the lack of the m(7)G46 modification and the hypo-modifications do not affect the Phe charging activity of tRNA(Phe), they cause a decrease in melting temperature of class I tRNA and degradation of tRNA(Phe) and tRNA(Ile). (35)S-Met incorporation into proteins revealed that protein synthesis in DeltatrmB cells is depressed above 70 degrees C. At 80 degrees C, the DeltatrmB strain exhibits a severe growth defect. Thus, the m(7)G46 modification is required for cell viability at high temperatures via a tRNA modification network, in which the m(7)G46 modification supports introduction of other modifications.

  2. Firefighting Module

    NASA Astrophysics Data System (ADS)

    1981-01-01

    Aviation Power Supply's mobile firefighting module called Firefly II is mounted on a trailer pulled by a pickup truck. Trailer unit has two three- inch water cannons, and the pickup carries a six inch cannon. Completely self contained, module pumps 3,000 gallons of water a minute from hydrants or open bodies of water. Stream can go as far as 400 feet or can be employed in a high-loft mode to reach the tops of tall refinery towers. Compact Firefly II weighs only 2,500 pounds when fully fueled. Key component is a specially designed two stage pump. Power for the pump is generated by a gas turbine engine. Module also includes an electronic/pump controller, multiple hose connections, up to 1,500 feet of hose and fuel for four hours operation. Firefly trailer can be backed onto specially-built large fireboat.

  3. Firefighting Module

    NASA Astrophysics Data System (ADS)

    1980-01-01

    Aviation Power Supply's mobile firefighting module called Firefly II is mounted on a trailer pulled by a pickup truck. Trailer unit has two three- inch water cannons, and the pickup carries a six inch cannon. Completely self contained, module pumps 3,000 gallons of water a minute from hydrants or open bodies of water. Stream can go as far as 400 feet or can be employed in a high-loft mode to reach the tops of tall refinery towers. Compact Firefly II weighs only 2,500 pounds when fully fueled. Key component is a specially designed two stage pump. Power for the pump is generated by a gas turbine engine. Module also includes an electronic/pump controller, multiple hose connections, up to 1,500 feet of hose and fuel for four hours operation. Firefly trailer can be backed onto specially-built large fireboat.

  4. Rapid Damage Mapping for the 2015 M7.8 Gorkha Earthquake using Synthetic Aperture Radar Data from COSMO-SkyMed and ALOS-2 Satellites

    NASA Astrophysics Data System (ADS)

    Yun, S. H.; Hudnut, K. W.; Owen, S. E.; Webb, F.; Simons, M.; Macdonald, A.; Sacco, P.; Gurrola, E. M.; Manipon, G.; Liang, C.; Fielding, E. J.; Milillo, P.; Hua, H.; Coletta, A.

    2015-12-01

    The April 25, 2015 M7.8 Gorkha earthquake caused more than 8,000 fatalities and widespread building damage in central Nepal. Four days after the earthquake, the Italian Space Agency's (ASI's) COSMO-SkyMed Synthetic Aperture Radar (SAR) satellite acquired data over Kathmandu area. Nine days after the earthquake, the Japan Aerospace Exploration Agency's (JAXA's) ALOS-2 SAR satellite covered larger area. Using these radar observations, we rapidly produced damage proxy maps derived from temporal changes in Interferometric SAR (InSAR) coherence. These maps were qualitatively validated through comparison with independent damage analyses by National Geospatial-Intelligence Agency (NGA) and the UNITAR's (United Nations Institute for Training and Research's) Operational Satellite Applications Programme (UNOSAT), and based on our own visual inspection of DigitalGlobe's WorldView optical pre- vs. post-event imagery. Our maps were quickly released to responding agencies and the public, and used for damage assessment, determining inspection/imaging priorities, and reconnaissance fieldwork.

  5. Cloning, expression and characterization of a novel cold-active and organic solvent-tolerant esterase from Monascus ruber M7.

    PubMed

    Guo, Hailun; Zhang, Yan; Shao, Yanchun; Chen, Wanping; Chen, Fusheng; Li, Mu

    2016-07-01

    Cold active esterases are a class of important biocatalysts that exhibit high activity at low temperatures. In this study, a search for putative cold-active esterase encoding genes from Monascus ruber M7 was performed. A cold-active esterase, named Lip10, was isolated, cloned, purified, and characterized. Amino acid sequence analysis reveals that Lip10 contained a conserved sequence motif Gly(173)-Xaa-Ser(175)-Xaa-Gly(177) that is also present in the majority of esterases and lipases. Phylogenetic analysis indicated that Lip10 was a novel microbial esterase. The lip10 gene was cloned and heterologously expressed in Escherichia coli BL21(DE3), resulting in the expression of an active and soluble protein that constituted 40 % of the total cell protein content. Lip10 maintained almost 50 % of its maximal activity at 4-10 °C, with optimal activity at 40 °C. Furthermore, Lip10 retained 184-216 % of its original activity, after incubation in 50 % (v/v) hydrophobic organic solvents for 24 h. The enzyme also exhibited high activity under alkaline conditions and good tolerance to metal ions in the reaction mixture. These results indicate that Lip10 may have potential uses in chemical synthesis and food processing industrial applications as an esterase. PMID:27209523

  6. Effect of multiple short highly energetic X-ray pulses on the synthesis of endoglucanase by a mutant strain of Trichoderma reesei-M7

    PubMed Central

    Gemishev, Orlin; Zapryanov, Stanislav; Blagoev, Alexander; Markova, Maya; Savov, Valentin

    2014-01-01

    Bioconversion of cellulose-containing substrate to glucose represents an important area of modern biotechnology. Enzymes for the degradation of the polysaccharide part of biomass have been produced, mostly by fungi belonging to genus Trichoderma. Studies were carried out with the mutant strain Trichoderma reesei-M7, a cellulase producer. Spores of the enzyme producer were irradiated with different doses of characteristic X-ray radiation from metallic tungsten (mainly the W Kα1 and Kα2 lines) with a high dose rate. The latter is a specific property of the dense plasma focus (DPF) device, which has pulsed operation and thus gives short and highly energetic pulses of multiple types of rays and particles. In this case, we focused our study on the influence of hard X-rays. The doses of X-rays absorbed by the spores varied in the range of approximately 5–11,000 mSv measured with thermoluminescent dosimeters (TLD). The influence of the applied doses in combination with exceptionally high dose rates (in the order of tens of millisieverts per microsecond) on the activity of the produced endoglucanase, amount of biomass and extra-cellular protein, was studied in batch cultivation conditions. In the dose range of 200–1200 mSv, some enhancement of endoglucanase activity was obtained: around 18%–32%, despite the drop of the biomass amount, compared with the untreated material. PMID:26019569

  7. Case study of a magnetic transient in NOAA 11429 observed by SDO/HMI during the M7.9 flare on 2012 march 13

    SciTech Connect

    Harker, Brian J.; Pevtsov, Alexei A. E-mail: apevtsov@nso.edu

    2013-12-01

    NOAA 11429 was the source of an M7.9 X-ray flare at the western solar limb (N18° W63°) on 2012 March 13 at 17:12 UT. Observations of the line-of-sight magnetic flux and the Stokes I and V profiles from which it is derived were carried out by the Solar Dynamics Observatory Helioseismic and Magnetic Imager (SDO/HMI) with a 45 s cadence over the full disk, at a spatial sampling of 0.''5. During flare onset, a transient patch of negative flux can be observed in SDO/HMI magnetograms to rapidly appear within the positive polarity penumbra of NOAA 11429. We present here a detailed study of this magnetic transient and offer interpretations as to whether this highly debated phenomenon represents a 'real' change in the structure of the magnetic field at the site of the flare, or is instead a product of instrumental/algorithmic artifacts related to particular SDO/HMI data reduction techniques.

  8. Implications for stress changes along the Motagua fault and other nearby faults using GPS and seismic constraints on the M=7.3 2009 Swan Islands earthquake

    NASA Astrophysics Data System (ADS)

    Graham, S. E.; Rodriguez, M.; Rogers, R. D.; Strauch, W.; Hernandez, D.; Demets, C.

    2010-12-01

    The May 28, 2009 M=7.3 Swan Islands earthquake off the north coast of Honduras caused significant damage in the northern part of the country, including seven deaths. This event, the largest in the region for several decades, ruptured the offshore continuation of the Motagua-Polochic fault system, whose 1976 earthquake (located several hundred kilometers to the southwest of the 2009 epicenter) caused more than 23,000 deaths in Central America and left homeless 20% of Guatemala’s population. We use elastic half-space modeling of coseismic offsets measured at 39 GPS stations in Honduras, El Salvador, and Guatemala to better understand the slip source of the recent Swan Islands earthquake. Measured offsets range from .32 meters at a campaign site near the Motagua fault in northern Honduras to 4 millimeters at five continuous sites in El Salvador. Coulomb stress calculations based on the estimated distribution of coseismic slip will be presented and compared to earthquake focal mechanisms and aftershock locations determined from a portable seismic network that was installed in northern Honduras after the main shock. Implications of the Swan Islands rupture for the seismically hazardous Motagua-Polochic fault system will be described.

  9. GPS and seismic constraints on the M = 7.3 2009 Swan Islands earthquake: implications for stress changes along the Motagua fault and other nearby faults

    NASA Astrophysics Data System (ADS)

    Graham, Shannon E.; DeMets, Charles; DeShon, Heather R.; Rogers, Robert; Maradiaga, Manuel Rodriguez; Strauch, Wilfried; Wiese, Klaus; Hernandez, Douglas

    2012-09-01

    We use measurements at 35 GPS stations in northern Central America and 25 seismometers at teleseismic distances to estimate the distribution of slip, source time function and Coulomb stress changes of the Mw = 7.3 2009 May 28, Swan Islands fault earthquake. This event, the largest in the region for several decades, ruptured the offshore continuation of the seismically hazardous Motagua fault of Guatemala, the site of the destructive Ms = 7.5 earthquake in 1976. Measured GPS offsets range from 308 millimetres at a campaign site in northern Honduras to 6 millimetres at five continuous sites in El Salvador. Separate inversions of geodetic and seismic data both indicate that up to ˜1 m of coseismic slip occurred along a ˜250-km-long rupture zone between the island of Roatan and the eastern limit of the 1976 M = 7.5 Motagua fault earthquake in Guatemala. Evidence for slip ˜250 km west of the epicentre is corroborated independently by aftershocks recorded by a local seismic network and by the high concentration of damage to structures in areas of northern Honduras adjacent to the western limit of the rupture zone. Coulomb stresses determined from the coseismic slip distribution resolve a maximum of 1 bar of stress transferred to the seismically hazardous Motagua fault and further indicate unclamping of normal faults along the northern shore of Honduras, where two M > 5 normal-faulting earthquakes and numerous small earthquakes were triggered by the main shock.

  10. The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA.

    PubMed

    Tomikawa, Chie; Hori, Hiroyuki

    2006-01-01

    Transfer RNA (m(7)G46) methyltransferase [TrmB] catalyses the transfer of methyl groups from S-adenosyl-L-methionine to the N(7)-atom of guanine at position 46 in tRNA. TrmB proteins from thermophilic bacteria such as Aquifex aeolicus have a long C-terminal region as compared to those from mesophilic bacteria. Further, N-terminal region observed in TrmB proteins from mesophiles is missing in A. aeolicus TrmB. Therefore, we considered that this distinct C-terminal region in A. aeolicus TrmB might compensate the N-terminal region in mesophile TrmB and function as a part of tRNA binding site. To confirm this idea, we deleted the C-terminal region by introduction of the stop codon at position 202. To our surprise, methyl-transfer assay using yeast tRNA(Phe) transcript clearly showed that the resultant mutant protein (Glu202Stop) had an enzymatic activity. Thus, the core domain of the A. aeolicus TrmB has a methyl-transfer activity.

  11. Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.

    PubMed

    Muneyoshi, Yuki; Matsumoto, Keisuke; Tomikawa, Chie; Toyooka, Takashi; Ochi, Anna; Masaoka, Takashi; Endo, Yaeta; Hori, Hiroyuki

    2007-01-01

    Yeast tRNA (m(7)G46) methyltransferase contains two protein subunits (Trm8 and Trm82). The enzyme catalyzes a methyl-transfer from S-adenosyl-L-methionine to the N(7) atom of guanine at position 46 in tRNA. We deviced synthesis of active Trm8-Trm82 heterodimer in a wheat germ cell-free translation system. When Trm8 or Trm82 mRNA were used for a synthesis, Trm8 or Trm82 protein could be synthesized. Upon mixing the synthesized Trm8 and Trm82 proteins, no active Trm8-Trm82 heterodimer was produced. Active Trm8-Trm82 heterodimer was only synthesized under conditions, in which both Trm8 and Trm82 mRNAs were co-translated. To address the RNA recognition mechanism of the Trm8-Trm82 complex, we investigated methyl acceptance activities of eight truncated yeast tRNA(Phe) transcripts. In this meeting, we demonstrate that yeast Trm8-Trm82 has stricter recognition requirements for the tRNA molecule as compared to the bacterial enzyme, TrmB.

  12. Earthquake Performance of Structures in the Philippines: A Post-event Assessment of the M7.2 October 2013 Bohol Earthquake

    NASA Astrophysics Data System (ADS)

    Naguit, M.; Cummins, P. R.; Bautista, B.; Edwards, M.; Ryu, H.; Jakab, M.

    2015-12-01

    A M7.2 inland earthquake occurred in Bohol, Philippines on the 15th of October 2013, associated with rupture along a newly-discovered thrust fault called the North Bohol Fault. While secondary hazards including lateral spreading, landslides, sinkholes, liquefaction, coastal uplift and subsidence have been observed, the impact was dominated by strong ground shaking that killed about 230 people and damaged over 70,000 buildings. Because of the extensive damage and the wide spread of intensities inferred to have shaken the island, the Bohol earthquake presents an important opportunity to improve knowledge of building fragility for the Philippines and similar countries. To this end we undertook a statistical building survey of over 18,000 damaged and undamaged structures located in urban and rural settings and at various inferred ground motion intensity levels. Building typology developed by structural engineers in the Philippines has been considered in classifying the structures based on structural materials and era of construction. The vast majority of the buildings are residential houses with wall types made of wooden materials, concrete hollow blocks or confined masonry. Tailored to assess the vulnerability of structures to earthquake loadings, this post-event analysis aims to validate and constrain the building fragility curves for seismic risk assessment. This can lead to more robust impact forecasts and higher priorities on seismic regulations and construction practices, applicable not only in Bohol but in other areas in the Philippines as well.

  13. PLASMOID EJECTIONS AND LOOP CONTRACTIONS IN AN ERUPTIVE M7.7 SOLAR FLARE: EVIDENCE OF PARTICLE ACCELERATION AND HEATING IN MAGNETIC RECONNECTION OUTFLOWS

    SciTech Connect

    Liu Wei; Chen Qingrong; Petrosian, Vahe

    2013-04-20

    Where particle acceleration and plasma heating take place in relation to magnetic reconnection is a fundamental question for solar flares. We report analysis of an M7.7 flare on 2012 July 19 observed by SDO/AIA and RHESSI. Bi-directional outflows in forms of plasmoid ejections and contracting cusp-shaped loops originate between an erupting flux rope and underlying flare loops at speeds of typically 200-300 km s{sup -1} up to 1050 km s{sup -1}. These outflows are associated with spatially separated double coronal X-ray sources with centroid separation decreasing with energy. The highest temperature is located near the nonthermal X-ray loop-top source well below the original heights of contracting cusps near the inferred reconnection site. These observations suggest that the primary loci of particle acceleration and plasma heating are in the reconnection outflow regions, rather than the reconnection site itself. In addition, there is an initial ascent of the X-ray and EUV loop-top source prior to its recently recognized descent, which we ascribe to the interplay among multiple processes including the upward development of reconnection and the downward contractions of reconnected loops. The impulsive phase onset is delayed by 10 minutes from the start of the descent, but coincides with the rapid speed increases of the upward plasmoids, the individual loop shrinkages, and the overall loop-top descent, suggestive of an intimate relation of the energy release rate and reconnection outflow speed.

  14. Strong ground motion synthesis for a M=7.2 earthquake in the Gulf of Corinth, Greece using Empirical Green`s functions

    SciTech Connect

    Hutchings, L.; Stavrakakis, G.N.; Ioannidou, E.; Wu, F.T.; Jarpe, S.; Kasameyer, P.

    1998-01-01

    We synthesize strong ground motion at three sites from a M=7.2 earthquake along the MW-trending Gulf of Cornith seismic zone. We model rupture along an 80 segment of the zone. The entire length of the fault, if activated at one time, can lead to an event comparable to that of the 1995 Kobe earthquake. With the improved digital data now routinely available, it becomes possible to use recordings of small earthquakes as empirical Green`s functions to synthesize potential ground motion for future large earthquakes. We developed a suite of 100 rupture scenarios for the earthquake and computed the commensurate strong ground motion time histories. We synthesized strong ground motion with physics-based solutions of earthquake rupture and applied physical bounds on rupture parameters. The synthesized ground motions obtained are source and site specific. By having a suite of rupture scenarios of hazardous earthquakes for a fixed magnitude and identifying the hazard to a site from the statistical distribution of engineering parameters, we have introduced a probabilistic component to the deterministic hazard calculation. The time histories suggested for engineering design are the ones that most closely match either the average or one standard deviation absolute accelerations response values.

  15. Geophysical investigation of the Denali fault and Alaska Range orogen within the aftershock zone of the October-November 2002, M = 7.9 Denali fault earthquake

    USGS Publications Warehouse

    Fisher, M.A.; Nokleberg, W.J.; Ratchkovski, N.A.; Pellerin, L.; Glen, J.M.; Brocher, T.M.; Booker, J.

    2004-01-01

    The aftershock zone of the 3 November 2002, M = 7.9 earthquake that ruptured along the right-slip Denali fault in south-central Alaska has been investigated by using gravity and magnetic, magnetotelluric, and deep-crustal, seismic reflection data as well as outcrop geology and earthquake seismology. Strong seismic reflections from within the Alaska Range orogen north of the Denali fault dip as steeply as 25°N and extend to depths as great as 20 km. These reflections outline a relict crustal architecture that in the past 20 yr has produced little seismicity. The Denali fault is nonreflective, probably because this fault dips steeply to vertical. The most intriguing finding from geophysical data is that earthquake aftershocks occurred above a rock body, with low electrical resistivity (>10 Ω·m), that is at depths below ∼10 km. Aftershocks of the Denali fault earthquake have mainly occurred shallower than 10 km. A high geothermal gradient may cause the shallow seismicity. Another possibility is that the low resistivity results from fluids, which could have played a role in locating the aftershock zone by reducing rock friction within the middle and lower crust.

  16. Vertical Crustal Stability in 23 Years Since the 1992 Cape Mendocino M 7.1 Earthquake: Benchmark Survey Results, Interpretations and Tectonic Implications

    NASA Astrophysics Data System (ADS)

    Vermeer, J.; Crawford, B.; Hemphill-Haley, M. A.

    2015-12-01

    In 1992, a M 7.1 earthquake occurred near Petrolia, CA within the area of the Mendocino triple junction and the southern terminus of the Cascadia subduction zone (CSZ). This earthquake resulted in ~1.4 m of coastal uplift, accompanied by inland subsidence. The focal mechanism of the earthquake indicated motion along a low-angle, east-dipping thrust plane, consistent with the subduction zone interface or a subsidiary fault. If the earthquake occurred on the megathrust itself, we might expect that high strain rates resulting from Gorda/North American plate convergence would cause measurable vertical interseismic deformation in the 23 years since the event. Along other subduction zones with historic ruptures, large amounts of deformation had occurred within 10-50 years after the mainshock, resulting in crustal recovery in a direction opposite that of the coseismic deformation. However, a subsidiary fault would not accumulate strain as quickly, thus little to no vertical deformation should be observed after just 23 years. Using high resolution static GPS relocation of benchmarks and comparing these positions to the 1992 leveling elevations, we have found that there has been little to no vertical deformation since the leveling in 1992. These results indicate that the 1992 event was likely not a subduction zone rupture but rather occurred along a low strain rate subsidiary fault.

  17. Thermionic modules

    DOEpatents

    King, Donald B.; Sadwick, Laurence P.; Wernsman, Bernard R.

    2002-06-18

    Modules of assembled microminiature thermionic converters (MTCs) having high energy-conversion efficiencies and variable operating temperatures manufactured using MEMS manufacturing techniques including chemical vapor deposition. The MTCs incorporate cathode to anode spacing of about 1 micron or less and use cathode and anode materials having work functions ranging from about 1 eV to about 3 eV. The MTCs also exhibit maximum efficiencies of just under 30%, and thousands of the devices and modules can be fabricated at modest costs.

  18. Ceftriaxone-Resistant Salmonella enterica Serotype Typhimurium Sequence Type 313 from Kenyan Patients Is Associated with the blaCTX-M-15 Gene on a Novel IncHI2 Plasmid

    PubMed Central

    Okoro, Chinyere; Kiiru, John; Omuse, Geoffrey; Langridge, Gemma; Kingsley, Robert A.; Dougan, Gordon; Revathi, Gunturu

    2015-01-01

    Multidrug-resistant bacteria pose a major challenge to the clinical management of infections in resource-poor settings. Although nontyphoidal Salmonella (NTS) bacteria cause predominantly enteric self-limiting illness in developed countries, NTS is responsible for a huge burden of life-threatening bloodstream infections in sub-Saharan Africa. Here, we characterized nine S. Typhimurium isolates from an outbreak involving patients who initially failed to respond to ceftriaxone treatment at a referral hospital in Kenya. These Salmonella enterica serotype Typhimurium isolates were resistant to ampicillin, chloramphenicol, cefuroxime, ceftriaxone, aztreonam, cefepime, sulfamethoxazole-trimethoprim, and cefpodoxime. Resistance to β-lactams, including to ceftriaxone, was associated with carriage of a combination of blaCTX-M-15, blaOXA-1, and blaTEM-1 genes. The genes encoding resistance to heavy-metal ions were borne on the novel IncHI2 plasmid pKST313, which also carried a pair of class 1 integrons. All nine isolates formed a single clade within S. Typhimurium ST313, the major clone of an ongoing invasive NTS epidemic in the region. This emerging ceftriaxone-resistant clone may pose a major challenge in the management of invasive NTS in sub-Saharan Africa. PMID:25779570

  19. Spatio-temporal evolution of the postseismic slip associated with the 2005 Miyagi-Oki earthquake (M7.2) estimated from geodetic and seismological data

    NASA Astrophysics Data System (ADS)

    Iinuma, T.; Miura, S.; Uchida, N.; Sato, M.; Saito, H.; Ishikawa, T.; Hino, R.; Matsuzawa, T.

    2010-12-01

    On August 16, 2005, a M7.2 earthquake occurred along the plate boundary off Miyagi Prefecture, northeastern Japan, where the Pacific plate is subducting beneath the overriding continental plate at a rate of about 80 mm/yr. There are at least three asperities that were ruptured during the 1978 Miyagi-Oki earthquake (M7.4) there, and one or two of them were reruptured during the 2005 earthquake. We estimated spatio-temporal evolution of the postseismic slip associated with the 2005 Miyagi-Oki earthquake using continuous land GPS and campaign ocean bottom GPS/acoustic observation data in order to investigate whether the strain accumulation process at the unruptured asperities are affected by the event in 2005 or not. Daily site coordinates were estimated using a PPP (Precise Point Positioning) strategy of GIPSY-OASISII Software based on the GPS data observed at continuous sites operated by GSI (Geospatial Information Authority of Japan) and Tohoku University. Data from January 2004 to December 2007 have been analyzed. The linear trends with annual and semi-annual variations for the period from January 1, 2004 to August 15, 2005, and co-seismic displacements due to the main shock were estimated by least square modeling and subtracted from the original onshore GPS time series. As to the ocean bottom displacement data, we calculated secular velocities at the offshore site locations from an interplate coupling model based on the linear trends of land GPS sites and subtracted them from the observed time series. We regarded that the detrended time series represented the deformation due to the afterslip of the 2005 earthquake and were inverted them to obtain spatiotemporal slip distribution on the plate boundary by using a time dependent inversion analysis. We applied an inversion method devised by Yagi and Kikuchi (2003) to estimate the evolution of the fault slip in both space and time. We also estimated spatiotemporal evolution of the aseismic slip based on the activities

  20. Are the source models of the M 7.1 1908 Messina Straits earthquake reliable? Insights from a novel inversion and a sensitivity analysis of levelling data

    NASA Astrophysics Data System (ADS)

    Aloisi, M.; Bruno, V.; Cannavò, F.; Ferranti, L.; Mattia, M.; Monaco, C.; Palano, M.

    2013-03-01

    For decades, many authors have attempted to define the location, geometry and kinematics of the causative fault for the 1908 December 28, M 7.1 earthquake that struck the Messina Straits between Sicily and Calabria (southern Italy). The coseismic displacement caused a predominant downwarping of the Straits and small land uplift away from it, which were documented by levelling surveys performed 1 yr before and immediately after the earthquake. Most of the source models based on inversion of levelling data suggested that the earthquake was caused by a low angle, east-dipping blind normal fault, whose upper projection intersects the Earth surface on the Sicilian (west) side of the Messina Straits. An alternative interpretation holds that the causative fault is one of the high-angle, west-dipping faults located in southern Calabria, on the eastern side of the Straits, and may in large part coincide with the mapped Armo Fault. Here, we critically review the levelling data with the aim of defining both their usefulness and limits in modelling the seismogenic fault. We demonstrate that the levelling data alone are not capable of discriminating between the two oppositely dipping fault models, and thus their role as a keystone for modellers is untenable. However, new morphotectonic and geodetic data indicate that the Armo Fault has very recent activity and is accumulating strain. The surface observations, together with appraisal of macroseismic intensity distribution, available seismic tomography and marine geophysical evidence, lends credit to the hypothesis that the Armo and possibly the S. Eufemia faults are part of a major crustal structure that slipped during the 1908 earthquake.

  1. Modelling macroseismic observations for historical earthquakes: the cases of the M = 7.0, 1954 Sofades and M = 6.8, 1957 Velestino events (central Greece)

    NASA Astrophysics Data System (ADS)

    Papazachos, Giannis; Papazachos, Costas; Skarlatoudis, Andreas; Kkallas, Harris; Lekkas, Efthimios

    2016-01-01

    We attempt to model the spatial distribution of the strong ground motion for the large M = 7.0, 1954 Sofades and M = 6.8, 1957 Velestino events (southern Thessaly basin, central Greece), using the macroseismic intensities ( I M M up to 9+) observed within the broader Thessaly area. For this reason, we employ a modified stochastic method realised by the EXSIM algorithm for extended sources, in order to reproduce the damage distribution of these earthquakes, in an attempt to combine existing earthquake information and appropriate scaling relations with surface geology and to investigate the efficiency of the available macroseismic data. For site-effects assessment, we use a new digital geological map of the broader Thessaly basin, where geological formations are grouped by age and mapped on appropriate NEHRP soil classes. Using the previous approach, we estimate synthetic time series for different rupture scenarios and employ various calibrating relations between PGA/PGV and macroseismic intensity, allowing the generation of synthetic (stochastic) isoseismals. Also, different site amplification factors proposed for the broader Aegean area, according to local geology, are tested. Finally, we also perform a sensitivity analysis of the fault location, taking into account the available neotectonic data for the broader southern Thessaly fault zone. The finally determined fault locations are different than previously proposed, in agreement with the available neotectonic information. The observed macroseismic intensities are in good agreement with the ones derived from the synthetic waveforms, verifying both the usefulness of the approach, as well as of the macroseismic data used. Finally, site-effects show clear correlation with the geological classification employed, with constant amplification factors for each soil class generally providing better results than generic transfer functions.

  2. Source properties of earthquakes near the Salton Sea triggered by the 16 October 1999 M 7.1 Hector Mine, California, earthquake

    USGS Publications Warehouse

    Hough, S.E.; Kanamori, H.

    2002-01-01

    We analyze the source properties of a sequence of triggered earthquakes that occurred near the Salton Sea in southern California in the immediate aftermath of the M 7.1 Hector Mine earthquake of 16 October 1999. The sequence produced a number of early events that were not initially located by the regional network, including two moderate earthquakes: the first within 30 sec of the P-wave arrival and a second approximately 10 minutes after the mainshock. We use available amplitude and waveform data from these events to estimate magnitudes to be approximately 4.7 and 4.4, respectively, and to obtain crude estimates of their locations. The sequence of small events following the initial M 4.7 earthquake is clustered and suggestive of a local aftershock sequence. Using both broadband TriNet data and analog data from the Southern California Seismic Network (SCSN), we also investigate the spectral characteristics of the M 4.4 event and other triggered earthquakes using empirical Green's function (EGF) analysis. We find that the source spectra of the events are consistent with expectations for tectonic (brittle shear failure) earthquakes, and infer stress drop values of 0.1 to 6 MPa for six M 2.1 to M 4.4 events. The estimated stress drop values are within the range observed for tectonic earthquakes elsewhere. They are relatively low compared to typically observed stress drop values, which is consistent with expectations for faulting in an extensional, high heat flow regime. The results therefore suggest that, at least in this case, triggered earthquakes are associated with a brittle shear failure mechanism. This further suggests that triggered earthquakes may tend to occur in geothermal-volcanic regions because shear failure occurs at, and can be triggered by, relatively low stresses in extensional regimes.

  3. The 2007 M7.7 Tocopilla northern Chile earthquake sequence: Implications for along-strike and downdip rupture segmentation and megathrust frictional behavior

    NASA Astrophysics Data System (ADS)

    Schurr, B.; Asch, G.; Rosenau, M.; Wang, R.; Oncken, O.; Barrientos, S.; Salazar, P.; Vilotte, J.-P.

    2012-05-01

    In 2007 a M7.7 earthquake occurred near the town of Tocopilla within the northern Chile seismic gap. Main shock slip, derived from coseismic surface deformation, was confined to the depth range between 30 and 55 km. We relocated ˜1100 events during six months before and one week after the main shock. Aftershock seismicity is first congruent to the main shock slip and then it spreads offshore west and northwest of Mejillones Peninsula (MP). Waveform modeling for 38 aftershocks reveals source mechanisms that are in the majority similar to the main shock. However, a few events appear to occur in the upper plate, some with extensional mechanisms. Juxtaposing the Tocopilla aftershocks with those following the neighboring 1995 Antofagasta earthquake produces a striking symmetry across an EW axis in the center of MP. Events seem to skirt around MP, probably due to a shallower Moho there. We suggest that the seismogenic coupling zone in northern Chile changes its frictional behavior in the downdip direction from unstable to mostly conditionally stable. For both earthquake sequences, aftershocks agglomerate in the conditionally stable region, whereas maximum inter-seismic slip deficit and co-seismic slip occurs in the unstable region. The boundary between the unstable and conditionally stable zones parallels the coastline. We identify a similar segmentation for other earthquakes in Chile and Peru, where the offshore segments break in great M > 8 earthquakes, and the onshore segments in smaller M < 8 earthquakes. Using critical taper analysis, we demonstrate a causal relationship between varying slip behavior on the interface and forearc wedge anatomy that can be attributed to spatial variations in the rate-dependency of friction.

  4. Coseismic surface displacements from optical and SAR image offset tracking, fault modeling and geomorphological analysis of the Sept. 24th, 2013 M7.7 Balochistan earthquake

    NASA Astrophysics Data System (ADS)

    Harrington, Jonathan; Wang, Teng; Feng, Guangcai; Akoglu, Ahmet; Jónsson, Sigurjón; Motagh, Mahdi

    2014-05-01

    The M 7.7 earthquake in the Balochistan province of Pakistan on September 24th, 2013 took place along a subsidiary fault in the transition area between the Makran accretionary prism and the Chaman transform fault. This tectonics of the Indian and Arabian plate collisions with Eurasia produce primarily oblique left-lateral strike slip in this region. In this work, measurements of displacement and mapping of the rupture trace are achieved through image correlation of Landsat 8 images and SAR offset tracking of TerraSAR-X data. Horizontal displacements from both methods and derived vertical displacements are used to constrain a fault rupture model for the earthquake. Preliminary results show a surprisingly uniform slip distribution with maximum displacement near the surface. The total fault rupture length is ~210 km, with up to 9 m of left-lateral strike-slip and 3 m of reverse faulting. Additionally, mapping of the rupture trace is made use of for geomorphological observations relating to slip rates and identification of transpressional and transtensional features. Our results indicate a mostly smooth rupture trace, with the presence of two restraining steps, a releasing bend and a 3 km long sliver where the surface rupture jumped from the foot of the range-front into the alluvial fans at their base. A small block at one of the restraining steps shows intermediate displacement in both data sets. At the southern end of the rupture we observe that displacement from the earthquake cuts across a fold-and-thrust belt of the Makran accretionary prism. Preliminary results show a minimum of 12 km of repeated section of the accretionary wedge, and within the southern repeated section we find an offset of 600 m between two parallel ridges across the rupture trace. We relate these observations to conceptual models of fault segmentation and growth.

  5. Crustal structure of the northern margin of the eastern Tien Shan, China, and its tectonic implications for the 1906 M~7.7 Manas earthquake

    USGS Publications Warehouse

    Wang, Chun-Yong; Yang, Zhu-En; Luo, Hai; Mooney, W.D.

    2004-01-01

    The Tien Shan orogenic belt is the most active intracontinental mountain belt in the world. We describe an 86-km-long N–S-trending deep seismic reflection profile (which passes through the southern Junggar basin) located on the northeastern Tien Shan piedmont. Two distinct anticlines beneath the northern margin of the Tien Shan are clearly imaged in the seismic section. In addition, we have imaged two detachment surfaces at depths of ∼7 and ∼16 km. The detachment surface at 16-km depth corresponds to the main detachment that converges with the steep angle reverse fault (the Junggar Southern Marginal Fault) on which the 1906 M~7.7 Manas earthquake occurred. A 12–14-km-thick sedimentary basin is imaged beneath the southern Junggar basin near Shihezi. The crust beneath the northern margin of the Tien Shan is 50–55-km thick, and decreases beneath the Junggar basin to 40–45-km thick. The crustal image of the deep seismic reflection profile is consistent with models derived from nearby seismic refraction data and Bouguer gravity anomalies in the same region. The faulting associated with the 1906 Manas earthquake also fits within the structural framework imaged by the seismic reflection profile. Present-day micro-seismicity shows a hypocentral depth-distribution between 5 and 35 km, with a peak at 20 km. We hypothesize that the 1906 Manas earthquake initiated at a depth of ∼20 km and propagated upwards, causing northward slip on the sub-horizontal detachments beneath the southern Junggar basin. Thus, in accord with regional geological mapping, the current shortening within the eastern Tien Shan is accommodated both by high-angle reverse faulting and detachment faulting that can be clearly imaged at depth in seismic reflection data.

  6. Spread of Enterobacter cloacae carrying blaNDM-1, blaCTX-M-15, blaSHV-12 and plasmid-mediated quinolone resistance genes in a surgical intensive care unit in Croatia.

    PubMed

    Petrosillo, N; Vranić-Ladavac, M; Feudi, C; Villa, L; Fortini, D; Barišić, N; Bedenić, B; Ladavac, R; D'Arezzo, S; Andrašević, A Tambić; Capone, A

    2016-03-01

    The objective of this study was to describe a hospital cluster of NDM-1-producing Enterobacter cloacae infections observed in the surgical intensive care unit (ICU) of a tertiary-care hospital at Pula, Croatia. NDM-1-producing E. cloacae strains isolated from clinical samples were screened by PCR for the presence of carbapenemases. Genetic relatedness of NDM-1-producing E. cloacae strains was determined by multilocus sequence typing (MLST). During the period October 2013 to April 2014, four patients, with overlapping hospital stay in the surgical ICU, developed severe infections caused by E. cloacae demonstrated to produce carbapenemases. According to MLST, all strains belonged to ST133 and were positive by PCR for the blaNDM-1 carbapenemase gene, for blaCTX-M-15 and blaSHV-12 extended-spectrum β-lactamase (ESBL) genes, and for blaTEM-1 and blaOXA-1 narrow-spectrum β-lactamase genes. They were negative for other carbapenemases genes including blaOXA-48, blaVIM and blaKPC as well as for AmpC and the armA and rmtB aminoglycoside resistance genes. All strains were positive for the HI2 replicon, suggesting that an IncHI2 plasmid is likely the plasmid carrying the blaNDM-1 gene. Infection control measures were implemented after the first case although they were not effective in avoiding spread of this organism to other patients in the surgical ICU. In conclusion, the evolving epidemiology of NDM-producing micro-organisms and the interspecies diffusion of this resistance mechanism to emerging pathogens such as E. cloacae necessitate the setting up of strong and urgent joint measures to control the spread of NDM carbapenemase especially in the ICU setting. PMID:27436392

  7. CTX-M-15 in combination with aac(6')-Ib-cr is the most prevalent mechanism of resistance both in Escherichia coli and Klebsiella pneumoniae, including K. pneumoniae ST258, in an ICU in Uruguay.

    PubMed

    Bado, Inés; Gutiérrez, Claudia; García-Fulgueiras, Virginia; Cordeiro, Nicolás F; Araújo Pirez, Lucía; Seija, Verónica; Bazet, Cristina; Rieppi, Gloria; Vignoli, Rafael

    2016-09-01

    The objectives of this study were (i) to determine the extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EcKp) clones circulating in an intensive care unit (ICU) in Uruguay between August 2010 and July 2011, (ii) to characterise the ESBL and plasmid-mediated quinolone resistance (PMQR) genes of the studied isolates and (iii) to determine the virulotype of the clinical isolates. Clinical and gut-colonising ESBL-EcKp from ICU patients were studied. Bacterial identification and antibiotic susceptibility determination were performed using a VITEK(®)2 system. Detection of ESBL, KPC and PMQR genes was performed by PCR and sequencing. Clonality was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In total, 54 ESBL-EcKp isolates (40 K. pneumoniae and 14 E. coli), with or without PMQR genes, were recovered from 30 of 68 inpatients. Forty-seven isolates were CTX-M-15-producers (36 as a single ESBL and 11 together with CTX-M-14). In addition, four isolates produced CTX-M-14, two produced CTX-M-2 and one produced SHV-5. No carbapenemases were detected either in E. coli or K. pneumoniae isolates. Among the ESBL-producing isolates, 42 also harboured PMQR genes: 27 aac(6')-Ib-cr; 14 aac(6')-Ib-cr and qnrB; and a single isolate carrying only qnrB. K. pneumoniae ST258, ST48 and ST16 and E. coli ST10 and ST405 were detected in 46/54 isolates, including 9 clinical isolates. In conclusion, non-KPC-producing K. pneumoniae ST258 harbouring different ESBL and PMQR genes was the main clone disseminated in the ICU. Extensive surveillance measures must be implemented to prevent the emergence of acquired plasmid-encoded blaKPC by ST258 K. pneumoniae.

  8. Firefighting Module

    NASA Astrophysics Data System (ADS)

    1984-01-01

    Firefly II pump module is NASA's Marshall Space Flight Center's commercial offshoot of a NASA/US Coast Guard program involving development of a lightweight, helicopter-transportable firefighting module for a quick response in combating shipboard or harbor fires. Operable on land or water, the Amphib One is equipped with 3 water cannons. When all 3 are operating, unit pumps more than 3,000 gallons a minute. Newly developed model used by U.S. Coast Guard can pump 5,000 gallons per minute. It was designed for applications such as firefighting onboard ship fires, emergency dockside water pumping, dewatering ships in danger of sinking, flood control, and emergency water supply at remote locations.

  9. Thermoelectric module

    DOEpatents

    Kortier, William E.; Mueller, John J.; Eggers, Philip E.

    1980-07-08

    A thermoelectric module containing lead telluride as the thermoelectric mrial is encapsulated as tightly as possible in a stainless steel canister to provide minimum void volume in the canister. The lead telluride thermoelectric elements are pressure-contacted to a tungsten hot strap and metallurgically bonded at the cold junction to iron shoes with a barrier layer of tin telluride between the iron shoe and the p-type lead telluride element.

  10. Linear modulator

    NASA Technical Reports Server (NTRS)

    1972-01-01

    A study of frequency division multiplexing (FDM) systems was made for the purpose of determining the system performance that can be obtained with available state of the art components. System performance was evaluated on the basis of past experience, system analysis, and component evaluation. The system study was specifically directed to the area of FDM systems using subcarrier channel frequencies from 4 kHz to 200 kHz and channel information bandwidths of dc to 1, 2, 4, 8, and 16 kHz. The evaluation also assumes that the demodulation will be from a tape recorder which produces frequency modulation of + or - 1% on the signal due to the tape recorder wow and flutter. For the modulation system it is assumed that the pilot and carrier channel frequencies are stable to within + or - .005% and that the FM on the channel carriers is negligible. The modulator system was evaluated for the temperature range of -20 degree to +85 degree while the demodulator system was evaluated for operation at room temperature.

  11. Earthquake source imaging by high-resolution array analysis at regional distances: the 2010 M7 Haiti earthquake as seen by the Venezuela National Seismic Network

    NASA Astrophysics Data System (ADS)

    Meng, L.; Ampuero, J. P.; Rendon, H.

    2010-12-01

    Back projection of teleseismic waves based on array processing has become a popular technique for earthquake source imaging,in particular to track the areas of the source that generate the strongest high frequency radiation. The technique has been previously applied to study the rupture process of the Sumatra earthquake and the supershear rupture of the Kunlun earthquakes. Here we attempt to image the Haiti earthquake using the data recorded by Venezuela National Seismic Network (VNSN). The network is composed of 22 broad-band stations with an East-West oriented geometry, and is located approximately 10 degrees away from Haiti in the perpendicular direction to the Enriquillo fault strike. This is the first opportunity to exploit the privileged position of the VNSN to study large earthquake ruptures in the Caribbean region. This is also a great opportunity to explore the back projection scheme of the crustal Pn phase at regional distances,which provides unique complementary insights to the teleseismic source inversions. The challenge in the analysis of the 2010 M7.0 Haiti earthquake is its very compact source region, possibly shorter than 30km, which is below the resolution limit of standard back projection techniques based on beamforming. Results of back projection analysis using the teleseismic USarray data reveal little details of the rupture process. To overcome the classical resolution limit we explored the Multiple Signal Classification method (MUSIC), a high-resolution array processing technique based on the signal-noise orthognality in the eigen space of the data covariance, which achieves both enhanced resolution and better ability to resolve closely spaced sources. We experiment with various synthetic earthquake scenarios to test the resolution. We find that MUSIC provides at least 3 times higher resolution than beamforming. We also study the inherent bias due to the interferences of coherent Green’s functions, which leads to a potential quantification

  12. Comparison of the Structurally Controlled Landslides Numerical Model Results to the M 7.2 2013 Bohol Earthquake Co-seismic Landslides

    NASA Astrophysics Data System (ADS)

    Macario Galang, Jan Albert; Narod Eco, Rodrigo; Mahar Francisco Lagmay, Alfredo

    2015-04-01

    The M 7.2 October 15, 2013 Bohol earthquake is the most destructive earthquake to hit the Philippines since 2012. The epicenter was located in Sagbayan municipality, central Bohol and was generated by a previously unmapped reverse fault called the "Inabanga Fault". Its name, taken after the barangay (village) where the fault is best exposed and was first seen. The earthquake resulted in 209 fatalities and over 57 billion USD worth of damages. The earthquake generated co-seismic landslides most of which were related to fault structures. Unlike rainfall induced landslides, the trigger for co-seismic landslides happen without warning. Preparedness against this type of landslide therefore, relies heavily on the identification of fracture-related unstable slopes. To mitigate the impacts of co-seismic landslide hazards, morpho-structural orientations or discontinuity sets were mapped in the field with the aid of a 2012 IFSAR Digital Terrain Model (DTM) with 5-meter pixel resolution and < 0.5 meter vertical accuracy. Coltop 3D software was then used to identify similar structures including measurement of their dip and dip directions. The chosen discontinuity sets were then keyed into Matterocking software to identify potential rock slide zones due to planar or wedged discontinuities. After identifying the structurally-controlled unstable slopes, the rock mass propagation extent of the possible rock slides was simulated using Conefall. The results were compared to a post-earthquake landslide inventory of 456 landslides. Out the total number of landslides identified from post-earthquake high-resolution imagery, 366 or 80% intersect the structural-controlled hazard areas of Bohol. The results show the potential of this method to identify co-seismic landslide hazard areas for disaster mitigation. Along with computer methods to simulate shallow landslides, and debris flow paths, located structurally-controlled unstable zones can be used to mark unsafe areas for settlement. The

  13. In-situ structure of the Nojima fault in the GSJ Hirabayashi hole, and evolution after the 1995 Kobe earthquake (M=7.2)

    NASA Astrophysics Data System (ADS)

    Pezard, P.; Pinettes, P.; Boullier, A.-M.; Hermitte, D.; Ito, H.; Fujimoto, K.; Ohtani, T.; Kiguchi, T.

    2003-04-01

    The Nojima fault located to the SE of Kobe, Japan, was drilled through at shallow depth one year after the 1995 Nanbu earthquake (M=7.2). Cores and downhole measurements collected from the in the Hirabayashi hole by the Geological Survey of Japan (GSJ) have been analyzed to describe the structure of the fault in relation to dynamic parameters such as fluid flow and deformation in the vicinity of the hole. The deformations of the borehole surface point to a 1.2 m-thick zone still deforming one year after the Nanbu earthquake. The 30 cm-thick gouge thought to include the plane of rupture is located at the top of this zone. The gouge itself is constituted with mm-scale structures including pseudo-tachylites. The layered nature of the gouge associated with local changes in grain size may provide the mechanism to store a significant overpressure before or after the earthquake, increasing locally the porosity and explaining the absence of shear resistance of the material. The in-situ physical properties are related, in this transpressional context, to changes in alteration and fracturing intensity of the granodiorite. Core analyses performed on minicores and at varying fluid salinity have provided measurements of porosity (Ø) electrical formation factor (F) and surface conductivity (Cs), both sensitive to deformation and alteration processes, for over 50 samples. Tortuosity (T=F.Ø) values derived from these analyses range for most samples from 10 to 30. Just above the fault gouge, T reaches values as high as 200, in a brecciated rock appearing to be healing after the earthquake and where neoformed crystals are imaged. The comparison of core data to in-situ measurements reveals also the presence of at least two types of pore fluids in the near vicinity of the fault. A low salinity fluid (Cw = 0.06 S/m) sampled in the hole in front of the fault zone, and a more saline pore fluid (Cw = 1.20 S/m) in the fresh granodiorite. This contrast suggests a relationship between

  14. Updated Rupture Model for the M7.8 October 28, 2012, Haida Gwaii Earthquake as Constrained by GPS-Observed Displacements

    NASA Astrophysics Data System (ADS)

    Nykolaishen, L.; Dragert, H.; Wang, K.; James, T. S.; de Lange Boom, B.; Schmidt, M.; Sinnott, D.

    2014-12-01

    The M7.8 low-angle thrust earthquake off the west coast of southern Haida Gwaii on October 28, 2012, provided Canadian scientists the opportunity to study a local large thrust earthquake and has provided important information towards an improved understanding of geohazards in coastal British Columbia. Most large events along the Pacific-North America boundary in this region have involved strike-slip motion, such as the 1949 M8.1 earthquake on the Queen Charlotte Fault. In contrast along the southern portion of Haida Gwaii, the young (~8 Ma) Pacific plate crust also underthrusts North America and has been viewed as a small-scale analogy of the Cascadia Subduction Zone. Initial seismic-based rupture models for this event were improved through inclusion of GPS observed coseismic displacements, which are as large as 115 cm of horizontal motion (SSW) and 30 cm of subsidence. Additional campaign-style GPS surveys have since been repeated by the Canadian Hydrographic Service (CHS) at seven vertical reference benchmarks throughout Haida Gwaii, significantly improving the coverage of coseismic displacement observations in the region. These added offsets were typically calculated by differencing a single occupation before and after the earthquake and preliminary displacement estimates are consistent with previous GPS observations from the Geological Survey of Canada. Addition of the CHS coseismic offset estimates may allow direct inversion of the GPS data to derive a purely GPS-based rupture model. To date, cumulative postseismic displacements at six sites indicate up to 6 cm of motion, varying in azimuth between SSW and SE. Preliminary postseismic timeseries curve fitting to date has utilized a double exponential function characteristic of mantle relaxation. The current postseismic trends also suggest afterslip on the deeper plate interface beneath central Haida Gwaii along with possible induced aseismic slip on a deeper segment of the Queen Charlotte Fault located offshore

  15. Tsunami modelling and source constraints from regional tide-gages and tilt measurements for the 2008 M=7.5, Tocopilla subduction earthquake

    NASA Astrophysics Data System (ADS)

    Hébert, H.; Boudin, F.; Bernard, P.; Peyrat, S.; Bejarpi, M.

    2008-12-01

    We focus on the small tsunami (a few tens of cm) generated by the November 2007, M=7.5 Tocopilla earthquake, which ruptured about 140 km of the southernmost part of the major, 1000 km long seismic gap of the northern Chile subduction. We first use the available regional tide-gage records, within 1000 km, at coastal stations and on nearby DART buoys (source of data: SHOA, Chile; University of Hawai; NOAA/NBDC) , to constrain the offshore, up-dip extension of the rupture, poorly constrained by the on-land records (GPS, InSAR, accelerograms). For this purpose, various source models, compatible with the geodetic/seismological data, are used as input for numerical calculations, including the best-fit, minimal seismological and geodetic inverted models. The vertical static displacement of the ocean floor is calculated using uniform, instantaneous dislocations in an elastic half-space. The tsunami wave is calculated using a finite-difference scheme solving the hydrodynamic equations, under the non linear shallow water assumption. Bathymetric data derived from the 1' GEBCO dataset are used. The time and amplitude of the first oscillations at the closest tide-gages constrain the offshore part of the source. The later part of the signal is persistent during more than one day, with dominant period of 40 to 45 minutes. Numerical simulation correctly reproduces these spectral characteristic as well as the persistence of the waves, which appear to be trapped within a narrow band (50 to 70 km wide) along at least the 800 km long coastline of the model, suggesting a very efficient trapping by the shallow area between the trench and the coast. This characteristic period was already present for previous tsunamis in this area (2001,1995,1877 major earthquakes) . At 200 to 300 km north to the epicentral area, tilt records from a long base tiltmeter and from broad-band seismometers (STS2) of the IPOC array, installed at short distances from the coast (3 to 25 km), show a clear signal

  16. Social vulnerability analysis of earthquake risk using HAZUS-MH losses from a M7.8 scenario earthquake on the San Andreas fault

    NASA Astrophysics Data System (ADS)

    Noriega, G. R.; Grant Ludwig, L.

    2010-12-01

    Natural hazards research indicates earthquake risk is not equitably distributed. Demographic differences are significant in determining the risks people encounter, whether and how they prepare for disasters, and how they fare when disasters occur. In this study, we analyze the distribution of economic and social losses in all 88 cities of Los Angeles County from the 2008 ShakeOut scenario earthquake. The ShakeOut scenario earthquake is a scientifically plausible M 7.8 scenario earthquake on the San Andreas fault that was developed and applied for regional earthquake preparedness planning and risk mitigation from a compilation of collaborative studies and findings by the 2007 Working Group on California Earthquake Probabilities (WGCEP). The scenario involved 1) developing a realistic scenario earthquake using the best available and most recent earthquake research findings, 2) estimation of physical damage, 3) estimation of social impact of the earthquake, and 4) identifying changes that will help to prevent a catastrophe due to an earthquake. Estimated losses from this scenario earthquake include 1,800 deaths and $213 billion dollars in economic losses. We use regression analysis to examine the relationship between potential city losses due to the ShakeOut scenario earthquake and the cities' demographic composition. The dependent variables are economic and social losses calculated in HAZUS-MH methodology for the scenario earthquake. The independent variables -median household income, tenure and race/ethnicity- have been identified as indicators of social vulnerability to natural disasters (Mileti, 1999; Cutter, 2006; Cutter & Finch, 2008). Preliminary Ordinary Least Squares (OLS) regression analysis of economic losses on race/ethnicity, income and tenure, indicates that cities with lower Hispanic population are associated with lower economic losses. Cities with higher Hispanic population are associated with higher economic losses, though this relationship is

  17. Photovoltaic module and module arrays

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Lenox, Carl J. S.; Culligan, Matthew; Danning, Matt

    2013-08-27

    A photovoltaic (PV) module including a PV device and a frame, The PV device has a PV laminate defining a perimeter and a major plane. The frame is assembled to and encases the laminate perimeter, and includes leading, trailing, and side frame members, and an arm that forms a support face opposite the laminate. The support face is adapted for placement against a horizontal installation surface, to support and orient the laminate in a non-parallel or tilted arrangement. Upon final assembly, the laminate and the frame combine to define a unitary structure. The frame can orient the laminate at an angle in the range of 3.degree.-7.degree. from horizontal, and can be entirely formed of a polymeric material. Optionally, the arm incorporates integral feature(s) that facilitate interconnection with corresponding features of a second, identically formed PV module.

  18. Photovoltaic module and module arrays

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Lenox, Carl J. S.; Culligan, Matthew; Danning, Matt

    2012-07-17

    A photovoltaic (PV) module including a PV device and a frame. The PV device has a PV laminate defining a perimeter and a major plane. The frame is assembled to and encases the laminate perimeter, and includes leading, trailing, and side frame members, and an arm that forms a support face opposite the laminate. The support face is adapted for placement against a horizontal installation surface, to support and orient the laminate in a non-parallel or tilted arrangement. Upon final assembly, the laminate and the frame combine to define a unitary structure. The frame can orient the laminate at an angle in the range of 3.degree.-7.degree. from horizontal, and can be entirely formed of a polymeric material. Optionally, the arm incorporates integral feature(s) that facilitate interconnection with corresponding features of a second, identically formed PV module.

  19. Comparative Genomic Analysis Reveals That the 20K and 38K Prophages in Listeria monocytogenes Serovar 4a Strains Lm850658 and M7 Contribute to Genetic Diversity but Not to Virulence.

    PubMed

    Fang, Chun; Cao, Tong; Shan, Ying; Xia, Ye; Xin, Yongping; Cheng, Changyong; Song, Houhui; Bowman, John; Li, Xiaoliang; Zhou, Xiangyang; Fang, Weihuan

    2016-01-01

    Listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. Initially, we found that the strain M7 was far less pathogenic than the strain Lm850658 though both are serovar 4a strains belonging to the lineage III. Comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. There are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% of the total genes in Lm850658 and 92.7% in M7. Lm850658 contains 33 specific genes, including a novel 20K prophage whereas strain M7 has 130 specific genes, including two large prophages (38K and 44K). To examine the roles of these specific prophages in pathogenicity, the 20K and 38K prophages were deleted from their respective strains. There were virtually no differences of pathogenicity between the deletion mutants and their parent strains, although some putative virulent factors like VirB4 are present in the 20K region or holin-lysin in the 38K region. In silico PCR analysis of 29 listeria genomes show that only strain SLCC2540 has the same 18 bp integration hotspot as Lm850658, whereas the sequence identity of their 20K prophages is very low (21.3%). The 38K and 44K prophages are located in two other different hotspots and are conserved in low virulent strains M7, HCC23, and L99. In conclusion, the 20K and 38K prophages of L. monocytogenes serovar 4a strains Lm850658 and M7 are not related to virulence but contribute to genetic diversity. PMID:26464378

  20. Comparative Genomic Analysis Reveals That the 20K and 38K Prophages in Listeria monocytogenes Serovar 4a Strains Lm850658 and M7 Contribute to Genetic Diversity but Not to Virulence.

    PubMed

    Fang, Chun; Cao, Tong; Shan, Ying; Xia, Ye; Xin, Yongping; Cheng, Changyong; Song, Houhui; Bowman, John; Li, Xiaoliang; Zhou, Xiangyang; Fang, Weihuan

    2016-01-01

    Listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. Initially, we found that the strain M7 was far less pathogenic than the strain Lm850658 though both are serovar 4a strains belonging to the lineage III. Comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. There are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% of the total genes in Lm850658 and 92.7% in M7. Lm850658 contains 33 specific genes, including a novel 20K prophage whereas strain M7 has 130 specific genes, including two large prophages (38K and 44K). To examine the roles of these specific prophages in pathogenicity, the 20K and 38K prophages were deleted from their respective strains. There were virtually no differences of pathogenicity between the deletion mutants and their parent strains, although some putative virulent factors like VirB4 are present in the 20K region or holin-lysin in the 38K region. In silico PCR analysis of 29 listeria genomes show that only strain SLCC2540 has the same 18 bp integration hotspot as Lm850658, whereas the sequence identity of their 20K prophages is very low (21.3%). The 38K and 44K prophages are located in two other different hotspots and are conserved in low virulent strains M7, HCC23, and L99. In conclusion, the 20K and 38K prophages of L. monocytogenes serovar 4a strains Lm850658 and M7 are not related to virulence but contribute to genetic diversity.

  1. Ionospheric anomalies related to the (M = 7.3), August 27, 2012, Puerto earthquake, (M = 6.8), August 30, 2012 Jan Mayen Island earthquake, and (M = 7.6), August 31, 2012, Philippines earthquake: two-dimensional principal component analysis.

    PubMed

    Lin, Jyh-Woei

    2013-01-01

    Two-dimensional principal component analysis (2DPCA) and principal component analysis (PCA) are used to examine the ionospheric total electron content (TEC) data during the time period from 00:00 on August 21 to 12: 45 on August 31 (UT), which are 10 days before the M = 7.6 Philippines earthquake at 12:47:34 on August 31, 2012 (UT) with the depth at 34.9 km. From the results by using 2DPCA, a TEC precursor of Philippines earthquake is found during the time period from 4:25 to 4:40 on August 28, 2012 (UT) with the duration time of at least 15 minutes. Another earthquake-related TEC anomaly is detectable for the time period from 04:35 to 04:40 on August 27, 2012 (UT) with the duration time of at least 5 minutes during the Puerto earthquake at 04: 37:20 on August 27, 2012 (UT) (M(w) = 7.3) with the depth at 20.3 km. The precursor of the Puerto earthquake is not detectable. TEC anomaly is not to be found related to the Jan Mayen Island earthquake (M w = 6.8) at 13:43:24 on August 30, 2012 (UT). These earthquake-related TEC anomalies are detectable by using 2DPCA rather than PCA. They are localized nearby the epicenters of the Philippines and Puerto earthquakes.

  2. Supported PV module assembly

    DOEpatents

    Mascolo, Gianluigi; Taggart, David F.; Botkin, Jonathan D.; Edgett, Christopher S.

    2013-10-15

    A supported PV assembly may include a PV module comprising a PV panel and PV module supports including module supports having a support surface supporting the module, a module registration member engaging the PV module to properly position the PV module on the module support, and a mounting element. In some embodiments the PV module registration members engage only the external surfaces of the PV modules at the corners. In some embodiments the assembly includes a wind deflector with ballast secured to a least one of the PV module supports and the wind deflector. An array of the assemblies can be secured to one another at their corners to prevent horizontal separation of the adjacent corners while permitting the PV modules to flex relative to one another so to permit the array of PV modules to follow a contour of the support surface.

  3. Lunar Module Ascent Stage

    NASA Technical Reports Server (NTRS)

    1969-01-01

    The Lunar Module 'Spider' ascent stage is photographed from the Command/Service Module on the fifth day of the Apollo 9 earth-orbital mission. The Lunar Module's descent stage had already been jettisoned.

  4. Static-stress impact of the 1992 Landers earthquake sequence on nucleation and slip at the site of the 1999 M=7.1 Hector Mine earthquake, southern California

    USGS Publications Warehouse

    Parsons, Tom; Dreger, Douglas S.

    2000-01-01

    The proximity in time (∼7 years) and space (∼20 km) between the 1992 M=7.3 Landers earthquake and the 1999 M=7.1 Hector Mine event suggests a possible link between the quakes. We thus calculated the static stress changes following the 1992 Joshua Tree/Landers/Big Bear earthquake sequence on the 1999 M=7.1 Hector Mine rupture plane in southern California. Resolving the stress tensor into rake-parallel and fault-normal components and comparing with changes in the post-Landers seismicity rate allows us to estimate a coefficient of friction on the Hector Mine plane. Seismicity following the 1992 sequence increased at Hector Mine where the fault was unclamped. This increase occurred despite a calculated reduction in right-lateral shear stress. The dependence of seismicity change primarily on normal stress change implies a high coefficient of static friction (µ≥0.8). We calculated the Coulomb stress change using µ=0.8 and found that the Hector Mine hypocenter was mildly encouraged (0.5 bars) by the 1992 earthquake sequence. In addition, the region of peak slip during the Hector Mine quake occurred where Coulomb stress is calculated to have increased by 0.5–1.5 bars. In general, slip was more limited where Coulomb stress was reduced, though there was some slip where the strongest stress decrease was calculated. Interestingly, many smaller earthquakes nucleated at or near the 1999 Hector Mine hypocenter after 1992, but only in 1999 did an event spread to become a M=7.1 earthquake.

  5. The Advanced Rapid Imaging and Analysis (ARIA) Project's Response to the April 25, 2015 M7.8 Nepal Earthquake: Rapid Measurements and Models for Science and Situational Awareness

    NASA Astrophysics Data System (ADS)

    Owen, S. E.; Fielding, E. J.; Yun, S. H.; Yue, H.; Polet, J.; Riel, B. V.; Liang, C.; Huang, M. H.; Webb, F.; Simons, M.; Moore, A. W.; Agram, P. S.; Barnhart, W. D.; Hua, H.; Liu, Z.; Milillo, P.; Sacco, G. F.; Rosen, P. A.; Manipon, G.

    2015-12-01

    On April 25, 2015, the M7.8 Gorkha earthquake struck Nepal and the city of Kathmandu. The quake caused a significant humanitarian crisis and killed more than 8,000 due to widespread building damage and triggered landslides throughout the region. This was the strongest earthquake to occur in the region since the 1934 Nepal-Bihar magnitude 8.0 quake caused more than 10,000 fatalities. In the days following the earthquake, the JPL/Caltech ARIA project produced coseismic GPS and SAR displacements, fault slip models, and damage assessments from SAR coherence change that were helpful in both understanding the event and in the response efforts. The ARIA project produced InSAR observations from two new SAR missions - JAXA's ALOS-2 and ESA's Sentinel 1a. The GPS coseismic displacements showed ~1.8 meters of southward motion and ~1.3 meters of uplift in Kathmandu. InSAR images of the displacement field and fault models show that the rupture extended 135 km southeast of the epicenter. The SAR imagery also confirmed that the fault slip did not extend to the surface, though localized offsets formed due to liquefaction. The GPS and SAR analysis has continued to image the large M7.3 aftershock and postseismic deformation. The damage assessments from coherence change were used by several organizations guiding the response effort, including the NGA, the World Bank, and OFDA/USAID. We will present imaging, modeling, and damage assessment results from the recent April 25, 2015 M7.8 earthquake in Nepal, and its largest aftershock, a M7.3 event on May 12, 2015. We also discuss how these data were used for understanding the event, guiding the response, and for educational outreach.

  6. Ballasted photovoltaic module and module arrays

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Danning, Matt

    2011-11-29

    A photovoltaic (PV) module assembly including a PV module and a ballast tray. The PV module includes a PV device and a frame. A PV laminate is assembled to the frame, and the frame includes an arm. The ballast tray is adapted for containing ballast and is removably associated with the PV module in a ballasting state where the tray is vertically under the PV laminate and vertically over the arm to impede overt displacement of the PV module. The PV module assembly can be installed to a flat commercial rooftop, with the PV module and the ballast tray both resting upon the rooftop. In some embodiments, the ballasting state includes corresponding surfaces of the arm and the tray being spaced from one another under normal (low or no wind) conditions, such that the frame is not continuously subjected to a weight of the tray.

  7. Module flammability research

    NASA Technical Reports Server (NTRS)

    Sugimura, R. S.; Otth, D. H.

    1985-01-01

    New materials were developed which show promise of fabricating modules that can pass the Underwriter Laboratories Class A burning brand test for fire ratable solar cell modules. It is concluded that fire resistant module design require special high temperature materials and constructions to achieve Class B and Class A ratings. Also, synergisms exist between back surface materials and module configuration.

  8. Module utilization committee

    NASA Technical Reports Server (NTRS)

    Volkmer, K.; Praver, G.

    1984-01-01

    Photovoltaic collector modules were declared surplus to the needs of the U.S. Dept. of Energy. The Module Utilization Committee was formed to make appropriate disposition of the surplus modules on a national basis and to act as a broker for requests for these modules originating outside of the National Photovoltaics Program.

  9. Modulational effects in accelerators

    SciTech Connect

    Satogata, T.

    1997-12-01

    We discuss effects of field modulations in accelerators, specifically those that can be used for operational beam diagnostics and beam halo control. In transverse beam dynamics, combined effects of nonlinear resonances and tune modulations influence diffusion rates with applied tune modulation has been demonstrated. In the longitudinal domain, applied RF phase and voltage modulations provide mechanisms for parasitic halo transport, useful in slow crystal extraction. Experimental experiences with transverse tune and RF modulations are also discussed.

  10. Almond brush module cutter

    SciTech Connect

    Zohns, M.A.; Jenkins, B.M.; Mehlschau, J.J.; Morrison, D.

    1983-06-01

    This paper addresses the design, construction, and evaluation of an almond brush module cutter. The module cutter is one link in a system which processes tree prunings for fuel and fiber. This system includes a modified cotton module builder, a module mover, the cutter, and a tub grinder. An economic analysis of the cutter is presented along with the problems involved in cutting brush modules.

  11. A short note on ground-motion recordings from the M 7.9 Wenchuan, China, earthquake and ground-motion prediction equations in the Central and Eastern United States

    USGS Publications Warehouse

    Wang, Z.; Lu, M.

    2011-01-01

    The 12 May 2008 Wenchuan earthquake (M 7.9) occurred along the western edge of the eastern China SCR and was well recorded by modern strong-motion instruments: 93 strong-motion stations within 1.4 to 300 km rupture distance recorded the main event. Preliminary comparisons show some similarities between ground-motion attenuation in the Wenchuan region and the central and eastern United States, suggesting that ground motions from the Wenchuan earthquake could be used as a database providing constraints for developing GMPEs for large earthquakes in the central and eastern United States.

  12. Commerical module test program

    NASA Technical Reports Server (NTRS)

    Smokier, M. I.

    1986-01-01

    The results are presented for a program of qualification testing of commercial crystalline silicon modules from nine manufacturers in five countries. The outcome demonstrated the effective role of the Block 5 Qualification Tests in the development of reliable modules.

  13. Integrated spatial light modulator

    NASA Astrophysics Data System (ADS)

    Yu, Dong X.; Storti, George M.; Wrigley, Charles Y.

    1992-08-01

    We described here an integrated spatial light modulator (SLM) employing Quantex electron trapping (ET) materials. The light modulation is accomplished by emission of ET material, upon incident coherent infrared light, where a pattern is written to by previous visible light excitation. The ET based spatial light modulators offer unique advantages over other SLM devices, such as capability of converting incoherent input to coherent light output and of integrating the modulator, the photodetector, and the memory into a single, rugged unit.

  14. Frequency modulated oscillator

    NASA Technical Reports Server (NTRS)

    Honnell, M. A. (Inventor)

    1977-01-01

    A frequency modulated push-pull oscillator in which the non-linear characteristic of varactors producing frequency modulation is compensated for by an opposite non-linear characteristic of a field effect transistor providing modulating bias to the varactors is described.

  15. Modulating lignin in plants

    SciTech Connect

    Apuya, Nestor; Bobzin, Steven Craig; Okamuro, Jack; Zhang, Ke

    2013-01-29

    Materials and methods for modulating (e.g., increasing or decreasing) lignin content in plants are disclosed. For example, nucleic acids encoding lignin-modulating polypeptides are disclosed as well as methods for using such nucleic acids to generate transgenic plants having a modulated lignin content.

  16. Rescue Manual. Module 5.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Instructional Materials Lab.

    This learner manual for rescuers covers the current techniques or practices required in the rescue service. The fifth of 10 modules contains information on hazardous materials. Key points, an introduction, and conclusion accompany substantive material in this module. In addition, the module contains a Department of Transportation guide chart on…

  17. Integrating Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Provides an overview of the complete National Energy Modeling System (NEMS) model, and includes brief descriptions of the modules with which the Integrating Module interacts. The emphasis and focus, however, is on the structure and function of the Integrating Module of NEMS.

  18. Spatial Light Amplifier Modulators

    NASA Technical Reports Server (NTRS)

    Eng, Sverre T.; Olsson, N. Anders

    1992-01-01

    Spatial light amplifier modulators (SLAM's) are conceptual devices that effect two-dimensional spatial modulation in optical computing and communication systems. Unlike current spatial light modulators, these provide gain. Optical processors incorporating SLAM's designed to operate in reflection or transmission mode. Each element of planar SLAM array is optical amplifier - surface-emitting diode laser. Array addressed electrically with ac modulating signals superimposed on dc bias currents supplied to lasers. SLAM device provides both desired modulation and enough optical gain to enable splitting of output signal into many optical fibers without excessive loss of power.

  19. Extended-spectrum beta-lactamase-producing Shiga toxin gene (stx1)-positive Escherichia coli O91:H14 carrying blaCTX-M-15 on an IncI1-ST31 plasmid isolated from a human patient in Germany.

    PubMed

    Arvand, Mardjan; Bettge-Weller, Gudrun; Fruth, Angelika; Uphoff, Helmut; Pfeifer, Yvonne

    2015-05-01

    In 2011, the Shiga toxin- and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O104:H4 caused a serious outbreak of gastroenteritis in Germany. This strain carried bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid. During screening of individuals at risk for acquisition of the epidemic E. coli O104:H4, we isolated another ESBL-producing and Shiga toxin-positive E. coli belonging to serotype O91:H14 from feces of a human patient. Interestingly, the patient also carried a further ESBL-producing but Shiga toxin-negative E. coli. Both strains harbored bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid, which was indistinguishable regarding size and plasmid restriction pattern from the plasmid of the epidemic E. coli O104:H4 strain. The patient had traveled to India 6 months prior to the isolation of the E. coli strains. This is the first report of an ESBL-producing, Shiga toxin-positive E. coli of serogroup O91. Our data suggest a high propensity of the IncI1-ST31 plasmid to spread in the human and/or animal population.

  20. Loadable Hypervisor Modules

    SciTech Connect

    Naughton, III, Thomas J; Vallee, Geoffroy R; Scott, Stephen L; Aderholdt, Ferrol

    2010-01-01

    This paper discusses the implementation of a new hypervisor mechanism for loading dynamic shared objects (modules) at runtime. These loadable hypervisor modules (LHM) are modeled after the loadable kernel modules used in Linux. We detail the current LHM implementation based on the Xen hypervisor. Potential use cases for this LHM mechanism include dynamic hypervisor instrumentation for debug tracing or performance analysis. We discuss the initial LHM prototype and future plans.

  1. Cavity enhanced terahertz modulation

    SciTech Connect

    Born, N.; Scheller, M.; Moloney, J. V.; Koch, M.

    2014-03-10

    We present a versatile concept for all optical terahertz (THz) amplitude modulators based on a Fabry-Pérot semiconductor cavity design. Employing the high reflectivity of two parallel meta-surfaces allows for trapping selected THz photons within the cavity and thus only a weak optical modulation of the semiconductor absorbance is required to significantly damp the field within the cavity. The optical switching yields to modulation depths of more than 90% with insertion efficiencies of 80%.

  2. FASTBUS Snoop Diagnostic Module

    SciTech Connect

    Walz, H.V.; Downing, R.

    1980-11-01

    Development of the FASTBUS Snoop Module, undertaken as part of the prototype program for the new interlaboratory data bus standard, is described. The Snoop Module resides on a FASTBUS crate segment and provides diagnostic monitoring and testing capability. Communication with a remote host computer is handled independent of FASTBUS through a serial link. The module consists of a high-speed ECL front-end to monitor and single-step FASTBUS cycles, a master-slave interface, and a control microprocessor with serial communication ports. Design details and performance specifications of the prototype module are reported. 9 figures, 1 table.

  3. Bubble memory module

    NASA Technical Reports Server (NTRS)

    Bohning, O. D.; Becker, F. J.

    1980-01-01

    Design, fabrication and test of partially populated prototype recorder using 100 kilobit serial chips is described. Electrical interface, operating modes, and mechanical design of several module configurations are discussed. Fabrication and test of the module demonstrated the practicality of multiplexing resulting in lower power, weight, and volume. This effort resulted in the completion of a module consisting of a fully engineered printed circuit storage board populated with 5 of 8 possible cells and a wire wrapped electronics board. Interface of the module is 16 bits parallel at a maximum of 1.33 megabits per second data rate on either of two interface buses.

  4. Bracket for photovoltaic modules

    SciTech Connect

    Ciasulli, John; Jones, Jason

    2014-06-24

    Brackets for photovoltaic ("PV") modules are described. In one embodiment, a saddle bracket has a mounting surface to support one or more PV modules over a tube, a gusset coupled to the mounting surface, and a mounting feature coupled to the gusset to couple to the tube. The gusset can have a first leg and a second leg extending at an angle relative to the mounting surface. Saddle brackets can be coupled to a torque tube at predetermined locations. PV modules can be coupled to the saddle brackets. The mounting feature can be coupled to the first gusset and configured to stand the one or more PV modules off the tube.

  5. Silicon photonic heater-modulator

    DOEpatents

    Zortman, William A.; Trotter, Douglas Chandler; Watts, Michael R.

    2015-07-14

    Photonic modulators, methods of forming photonic modulators and methods of modulating an input optical signal are provided. A photonic modulator includes a disk resonator having a central axis extending along a thickness direction of the disk resonator. The disk resonator includes a modulator portion and a heater portion. The modulator portion extends in an arc around the central axis. A PN junction of the modulator portion is substantially normal to the central axis.

  6. Membrane module assembly

    DOEpatents

    Kaschemekat, Jurgen

    1994-01-01

    A membrane module assembly adapted to provide a flow path for the incoming feed stream that forces it into prolonged heat-exchanging contact with a heating or cooling mechanism. Membrane separation processes employing the module assembly are also disclosed. The assembly is particularly useful for gas separation or pervaporation.

  7. Cosmetology. Computerized Learning Modules.

    ERIC Educational Resources Information Center

    Finnerty, Kathy, Ed.

    Intended to help reading-limited students meet course objectives, these 11 modules are based on instructional materials in cosmetology that have a higher readability equivalent. Modules cover bacteriology, chemical waving, scalp and hair massage, chemistry, hair shaping, hairstyling, chemical hair relaxing, hair coloring, skin and scalp,…

  8. Logs Perl Module

    2007-04-04

    A perl module designed to read and parse the voluminous set of event or accounting log files produced by a Portable Batch System (PBS) server. This module can filter on date-time and/or record type. The data can be returned in a variety of formats.

  9. Module Safety Issues (Presentation)

    SciTech Connect

    Wohlgemuth, J.

    2012-02-01

    Description of how to make PV modules so that they are less likely to turn into safety hazards. Making modules inherently safer with minimum additional cost is the preferred approach for PV. Safety starts with module design to ensure redundancy within the electrical circuitry to minimize open circuits and proper mounting instructions to prevent installation related ground faults. Module manufacturers must control the raw materials and processes to ensure that that every module is built like those qualified through the safety tests. This is the reason behind the QA task force effort to develop a 'Guideline for PV Module Manufacturing QA'. Periodic accelerated stress testing of production products is critical to validate the safety of the product. Combining safer PV modules with better systems designs is the ultimate goal. This should be especially true for PV arrays on buildings. Use of lower voltage dc circuits - AC modules, DC-DC converters. Use of arc detectors and interrupters to detect arcs and open the circuits to extinguish the arcs.

  10. Lunar Module Communications

    NASA Technical Reports Server (NTRS)

    Interbartolo, Michael A.

    2009-01-01

    This slide presentation reviews the Apollo lunar module communications. It describes several changes in terminology from the Apollo era to more recent terms. It reviews: (1) Lunar Module Antennas and Functions (2). Earth Line of Sight Communications Links (3) No Earth Line of Sight Communications Links (4) Lunar Surface Communications Links (5) Signal-Processing Assembly (6) Instrumentation System (7) Some Communications Problems Encountered

  11. Membrane module assembly

    DOEpatents

    Kaschemekat, J.

    1994-03-15

    A membrane module assembly is described which is adapted to provide a flow path for the incoming feed stream that forces it into prolonged heat-exchanging contact with a heating or cooling mechanism. Membrane separation processes employing the module assembly are also disclosed. The assembly is particularly useful for gas separation or pervaporation. 2 figures.

  12. Principles of Teaching. Module.

    ERIC Educational Resources Information Center

    Rhoades, Joseph W.

    This module on principles of teaching is 1 in a series of 10 modules written for vocational education teacher education programs. It is designed to enable the teacher to do the following: (1) identify subject matter and integrate that subject matter with thought-provoking questions; (2) organize and demonstrate good questioning techniques; and (3)…

  13. Human Development Student Modules.

    ERIC Educational Resources Information Center

    South Carolina State Dept. of Education, Columbia. Office of Vocational Education.

    This set of 61 student learning modules deals with various topics pertaining to human development. The modules, which are designed for use in performance-based vocational education programs, each contain the following components: an introduction for the student, a performance objective, a variety of learning activities, content information, a…

  14. A Search for Coronal Emission at the Bottom of the Main-Sequence: Stars and Brown Dwarf Candidates with Spectral Types Later than M7 and the Rotation-Activity Relation

    NASA Technical Reports Server (NTRS)

    Stringfellow, Guy

    2004-01-01

    This program intended to test whether the lowest mass stars at the bottom end of the main sequence and the lower mass brown dwarfs have coronae. If they have coronae, what are the coronal characteristics and what drives them? In the classical dynamo picture, the closed magnetic loop structure is generated near the boundary of the convective envelope and the radiative core. Stars with mass below 0.30 Msun however are fully convective, and the nature of the dynamo responsible for the generation of the coronae in this regime is poorly understood. Previous results from the ROSAT mission (e.g., Fleming et al. 1993, 1995; Schmitt et al. 1995) had confirmed three very important characteristics of M-star coronae: (1) a very high percentage of all M dwarfs have coronae (of order 85% in the local 7 pc sample), (2) those M dwarfs showing high chromospheric activity, such as having the Balmer series in emission or large/numerous optical flaring, indeed exhibit the highest coronal activity, and (3) that the maximum saturation boundary in X-ray luminosity, which amounts to 0.0001-0.001 for Lx/Lbol for the dMe stars, extends down to the current detection limit, through spectral types M7. It was likely that the incompleteness noted for result (1) above was simply a detection limit problem; for more distant sources, the X-ray fainter dM stars will drop below detection thresholds before the more X-ray luminous dMe stars. The latest stars for which direct detection of the corona had been successful were of spectral type dM7 (e.g., VB8, LHS 3003). This program proposed to obtain ROSAT HRI observations for a large number of the coolest known (at that time) stars at the bottom of the main-sequence, which had spectral types of M9 or later. Three stars were approved for observations with ROSAT-HRI totaling 180 ksec. The goal was to obtain X-ray detections or low upper limits for the three approved stars.

  15. Solar energy modulator

    NASA Technical Reports Server (NTRS)

    Hale, R. R. (Inventor); Mcdougal, A. R.

    1984-01-01

    A module is described with a receiver having a solar energy acceptance opening and supported by a mounting ring along the optic axis of a parabolic mirror in coaxial alignment for receiving solar energy from the mirror, and a solar flux modulator plate for varying the quantity of solar energy flux received by the acceptance opening of the module. The modulator plate is characterized by an annular, plate-like body, the internal diameter of which is equal to or slightly greater than the diameter of the solar energy acceptance opening of the receiver. Slave cylinders are connected to the modulator plate for supporting the plate for axial displacement along the axis of the mirror, therby shading the opening with respect to solar energy flux reflected from the surface of the mirror to the solar energy acceptance opening.

  16. Interpretation of earthquake-induced landslides triggered by the 12 May 2008, M7.9 Wenchuan earthquake in the Beichuan area, Sichuan Province, China using satellite imagery and Google Earth

    USGS Publications Warehouse

    Sato, H.P.; Harp, E.L.

    2009-01-01

    The 12 May 2008 M7.9 Wenchuan earthquake in the People's Republic of China represented a unique opportunity for the international community to use commonly available GIS (Geographic Information System) tools, like Google Earth (GE), to rapidly evaluate and assess landslide hazards triggered by the destructive earthquake and its aftershocks. In order to map earthquake-triggered landslides, we provide details on the applicability and limitations of publicly available 3-day-post- and pre-earthquake imagery provided by GE from the FORMOSAT-2 (formerly ROCSAT-2; Republic of China Satellite 2). We interpreted landslides on the 8-m-resolution FORMOSAT-2 image by GE; as a result, 257 large landslides were mapped with the highest concentration along the Beichuan fault. An estimated density of 0.3 landslides/km2 represents a minimum bound on density given the resolution of available imagery; higher resolution data would have identified more landslides. This is a preliminary study, and further study is needed to understand the landslide characteristics in detail. Although it is best to obtain landslide locations and measurements from satellite imagery having high resolution, it was found that GE is an effective and rapid reconnaissance tool. ?? 2009 Springer-Verlag.

  17. Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases

    PubMed Central

    Husain, Nilofer; Tkaczuk, Karolina L.; Tulsidas, Shenoy Rajesh; Kaminska, Katarzyna H.; Čubrilo, Sonja; Sivaraman, J.

    2010-01-01

    Sgm (Sisomicin-gentamicin methyltransferase) from antibiotic-producing bacterium Micromonospora zionensis is an enzyme that confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA. Sgm belongs to the aminoglycoside resistance methyltransferase (Arm) family of enzymes that have been recently found to spread by horizontal gene transfer among disease-causing bacteria. Structural characterization of Arm enzymes is the key to understand their mechanism of action and to develop inhibitors that would block their activity. Here we report the structure of Sgm in complex with cofactors S-adenosylmethionine (AdoMet) and S-adenosylhomocysteine (AdoHcy) at 2.0 and 2.1 Å resolution, respectively, and results of mutagenesis and rRNA footprinting, and protein-substrate docking. We propose the mechanism of methylation of G1405 by Sgm and compare it with other m7G methyltransferases, revealing a surprising diversity of active sites and binding modes for the same basic reaction of RNA modification. This analysis can serve as a stepping stone towards developing drugs that would specifically block the activity of Arm methyltransferases and thereby re-sensitize pathogenic bacteria to aminoglycoside antibiotics. PMID:20194115

  18. Photovoltaic module and interlocked stack of photovoltaic modules

    DOEpatents

    Wares, Brian S.

    2014-09-02

    One embodiment relates to an arrangement of photovoltaic modules configured for transportation. The arrangement includes a plurality of photovoltaic modules, each photovoltaic module including a frame. A plurality of individual male alignment features and a plurality of individual female alignment features are included on each frame. Adjacent photovoltaic modules are interlocked by multiple individual male alignment features on a first module of the adjacent photovoltaic modules fitting into and being surrounded by corresponding individual female alignment features on a second module of the adjacent photovoltaic modules. Other embodiments, features and aspects are also disclosed.

  19. Optical modulator system

    NASA Technical Reports Server (NTRS)

    Brand, J.

    1972-01-01

    The fabrication, test, and delivery of an optical modulator system which will operate with a mode-locked Nd:YAG laser indicating at either 1.06 or 0.53 micrometers is discussed. The delivered hardware operates at data rates up to 400 Mbps and includes a 0.53 micrometer electrooptic modulator, a 1.06 micrometer electrooptic modulator with power supply and signal processing electronics with power supply. The modulators contain solid state drivers which accept digital signals with MECL logic levels, temperature controllers to maintain a stable thermal environment for the modulator crystals, and automatic electronic compensation to maximize the extinction ratio. The modulators use two lithium tantalate crystals cascaded in a double pass configuration. The signal processing electronics include encoding electronics which are capable of digitizing analog signals between the limit of + or - 0.75 volts at a maximum rate of 80 megasamples per second with 5 bit resolution. The digital samples are serialized and made available as a 400 Mbps serial NRZ data source for the modulators. A pseudorandom (PN) generator is also included in the signal processing electronics. This data source generates PN sequences with lengths between 31 bits and 32,767 bits in a serial NRZ format at rates up to 400 Mbps.

  20. Amplitude Modulator Chassis

    SciTech Connect

    Erbert, G

    2009-09-01

    The Amplitude Modulator Chassis (AMC) is the final component in the MOR system and connects directly to the PAM input through a 100-meter fiber. The 48 AMCs temporally shape the 48 outputs of the MOR using an arbitrary waveform generator coupled to an amplitude modulator. The amplitude modulation element is a two stage, Lithium Niobate waveguide device, where the intensity of the light passing through the device is a function of the electrical drive applied. The first stage of the modulator is connected to a programmable high performance Arbitrary Waveform Generator (AWG) consisting of 140 impulse generators space 250 ps apart. An arbitrary waveform is generated by independently varying the amplitude of each impulse generator and then summing the impulses together. In addition to the AWG a short pulse generator is also connected to the first stage of the modulator to provide a sub 100-ps pulse used for timing experiments. The second stage of the modulator is connect to a square pulse generator used to further attenuate any pre or post pulse light passing through the first stage of the modulator. The fast rise and fall time of the square pulse generator is also used to produce fast rise and fall times of the AWG by clipping the AWG pulse. For maximum extinction, a pulse bias voltage is applied to each stage of the modulator. A pulse voltage is applied as opposed to a DC voltage to prevent charge buildup on the modulator. Each bias voltage is adjustable to provide a minimum of 50-dB extinction. The AMC is controlled through ICCS to generate the desired temporal pulse shape. This process involves a closed-loop control algorithm, which compares the desired temporal waveform to the produced optical pulse, and iterates the programming of the AWG until the two waveforms agree within an allowable tolerance.

  1. Autonomous cotton module forming system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cotton producers often have difficulty finding adequate labor during harvest. Module builder operators are often inexperienced and may build poorly shaped modules. Equipment manufacturers have recently introduced harvesters with on-board module building capabilities to reduce labor requirements; h...

  2. Water heater control module

    DOEpatents

    Hammerstrom, Donald J

    2013-11-26

    An advanced electric water heater control system that interfaces with a high temperature cut-off thermostat and an upper regulating thermostat. The system includes a control module that is electrically connected to the high-temperature cut-off thermostat and the upper regulating thermostat. The control module includes a switch to open or close the high-temperature cut-off thermostat and the upper regulating thermostat. The control module further includes circuitry configured to control said switch in response to a signal selected from the group of an autonomous signal, a communicated signal, and combinations thereof.

  3. Sonication standard laboratory module

    DOEpatents

    Beugelsdijk, Tony; Hollen, Robert M.; Erkkila, Tracy H.; Bronisz, Lawrence E.; Roybal, Jeffrey E.; Clark, Michael Leon

    1999-01-01

    A standard laboratory module for automatically producing a solution of cominants from a soil sample. A sonication tip agitates a solution containing the soil sample in a beaker while a stepper motor rotates the sample. An aspirator tube, connected to a vacuum, draws the upper layer of solution from the beaker through a filter and into another beaker. This beaker can thereafter be removed for analysis of the solution. The standard laboratory module encloses an embedded controller providing process control, status feedback information and maintenance procedures for the equipment and operations within the standard laboratory module.

  4. Optical modulator including grapene

    DOEpatents

    Liu, Ming; Yin, Xiaobo; Zhang, Xiang

    2016-06-07

    The present invention provides for a one or more layer graphene optical modulator. In a first exemplary embodiment the optical modulator includes an optical waveguide, a nanoscale oxide spacer adjacent to a working region of the waveguide, and a monolayer graphene sheet adjacent to the spacer. In a second exemplary embodiment, the optical modulator includes at least one pair of active media, where the pair includes an oxide spacer, a first monolayer graphene sheet adjacent to a first side of the spacer, and a second monolayer graphene sheet adjacent to a second side of the spacer, and at least one optical waveguide adjacent to the pair.

  5. Modulated curvaton decay

    SciTech Connect

    Assadullahi, Hooshyar; Wands, David; Firouzjahi, Hassan; Namjoo, Mohammad Hossein E-mail: firouz@mail.ipm.ir E-mail: david.wands@port.ac.uk

    2013-03-01

    We study primordial density perturbations generated by the late decay of a curvaton field whose decay rate may be modulated by the local value of another isocurvature field, analogous to models of modulated reheating at the end of inflation. We calculate the primordial density perturbation and its local-type non-Gaussianity using the sudden-decay approximation for the curvaton field, recovering standard curvaton and modulated reheating results as limiting cases. We verify the Suyama-Yamaguchi inequality between bispectrum and trispectrum parameters for the primordial density field generated by multiple field fluctuations, and find conditions for the bound to be saturated.

  6. Module bay with directed flow

    DOEpatents

    Torczynski, John R.

    2001-02-27

    A module bay requires less cleanroom airflow. A shaped gas inlet passage can allow cleanroom air into the module bay with flow velocity preferentially directed toward contaminant rich portions of a processing module in the module bay. Preferential gas flow direction can more efficiently purge contaminants from appropriate portions of the module bay, allowing a reduced cleanroom air flow rate for contaminant removal. A shelf extending from an air inlet slit in one wall of a module bay can direct air flowing therethrough toward contaminant-rich portions of the module bay, such as a junction between a lid and base of a processing module.

  7. Basic memory module

    NASA Technical Reports Server (NTRS)

    Tietze, F. C.

    1974-01-01

    Construction and electrical characterization of the 4096 x 2-bit Basic Memory Module (BMM) are reported for the Space Ultrareliable Modular Computer (SUMC) program. The module uses four 2K x 1-bit N-channel FET, random access memory chips, called array chips, and two sense amplifier chips, mounted and interconnected on a ceramic substrate. Four 5% tolerance power supplies are required. At the Module, the address, chip select, and array select lines require a 0-8.5 V MOS signal level. The data output, read-strobe, and write-enable lines operate at TTl levels. Although the module is organized as 4096 x 2 bits, it can be used in a 8196 x 1-bit application with appropriate external connections. A 4096 x 1-bit organization can be obtained by depopulating chips.

  8. GREET Pretreatment Module

    SciTech Connect

    Adom, Felix K.; Dunn, Jennifer B.; Han, Jeongwoo

    2014-09-01

    A wide range of biofuels and biochemicals can be produced from biomass via different pretreatment technologies that yield sugars. This report documents the material and energy flows that occur when fermentable sugars from four lignocellulosic feedstocks (corn stover, miscanthus, switchgrass, and poplar) are produced via dilute acid pretreatment and ammonia fiber expansion. These flows are documented for inclusion in the pretreatment module of the Greenhouses Gases, Regulated Emissions, and Energy Use in Transportation (GREET) model. Process simulations of each pretreatment technology were developed in Aspen Plus. Material and energy consumption data from Aspen Plus were then compiled in the GREET pretreatment module. The module estimates the cradle-to-gate fossil energy consumption (FEC) and greenhouse gas (GHG) emissions associated with producing fermentable sugars. This report documents the data and methodology used to develop this module and the cradle-to-gate FEC and GHG emissions that result from producing fermentable sugars.

  9. Module encapsulation technology

    NASA Technical Reports Server (NTRS)

    Willis, P.

    1986-01-01

    The identification and development techniques for low-cost module encapsulation materials were reviewed. Test results were displayed for a variety of materials. The improved prospects for modeling encapsulation systems for life prediction were reported.

  10. Digital optical conversion module

    DOEpatents

    Kotter, D.K.; Rankin, R.A.

    1988-07-19

    A digital optical conversion module used to convert an analog signal to a computer compatible digital signal including a voltage-to-frequency converter, frequency offset response circuitry, and an electrical-to-optical converter. Also used in conjunction with the digital optical conversion module is an optical link and an interface at the computer for converting the optical signal back to an electrical signal. Suitable for use in hostile environments having high levels of electromagnetic interference, the conversion module retains high resolution of the analog signal while eliminating the potential for errors due to noise and interference. The module can be used to link analog output scientific equipment such as an electrometer used with a mass spectrometer to a computer. 2 figs.

  11. Digital optical conversion module

    DOEpatents

    Kotter, Dale K.; Rankin, Richard A.

    1991-02-26

    A digital optical conversion module used to convert an analog signal to a computer compatible digital signal including a voltage-to-frequency converter, frequency offset response circuitry, and an electrical-to-optical converter. Also used in conjunction with the digital optical conversion module is an optical link and an interface at the computer for converting the optical signal back to an electrical signal. Suitable for use in hostile environments having high levels of electromagnetic interference, the conversion module retains high resolution of the analog signal while eliminating the potential for errors due to noise and interference. The module can be used to link analog output scientific equipment such as an electrometer used with a mass spectrometer to a computer.

  12. Columbus pressurized module verification

    NASA Technical Reports Server (NTRS)

    Messidoro, Piero; Comandatore, Emanuele

    1986-01-01

    The baseline verification approach of the COLUMBUS Pressurized Module was defined during the A and B1 project phases. Peculiarities of the verification program are the testing requirements derived from the permanent manned presence in space. The model philosophy and the test program have been developed in line with the overall verification concept. Such critical areas as meteoroid protections, heat pipe radiators and module seals are identified and tested. Verification problem areas are identified and recommendations for the next development are proposed.

  13. Firefighting module development

    NASA Astrophysics Data System (ADS)

    Burns, R. A.

    1981-10-01

    The firefighting module is a lightweight, compact, self contained, helicopter-transportable unit for fighting harbor and other specialty fires as well as for use in emergency water pumping applications. Units were fabricated and tested. A production type unit is undergoing an inservice evaluation and demonstration program at the port of St Louis. The primary purpose is to promote enhanced harbor fire protection at inland and coastal ports. The module and its development are described.

  14. Photovoltaic module reliability workshop

    SciTech Connect

    Mrig, L.

    1990-01-01

    The paper and presentations compiled in this volume form the Proceedings of the fourth in a series of Workshops sponsored by Solar Energy Research Institute (SERI/DOE) under the general theme of photovoltaic module reliability during the period 1986--1990. The reliability Photo Voltaic (PV) modules/systems is exceedingly important along with the initial cost and efficiency of modules if the PV technology has to make a major impact in the power generation market, and for it to compete with the conventional electricity producing technologies. The reliability of photovoltaic modules has progressed significantly in the last few years as evidenced by warranties available on commercial modules of as long as 12 years. However, there is still need for substantial research and testing required to improve module field reliability to levels of 30 years or more. Several small groups of researchers are involved in this research, development, and monitoring activity around the world. In the US, PV manufacturers, DOE laboratories, electric utilities and others are engaged in the photovoltaic reliability research and testing. This group of researchers and others interested in this field were brought together under SERI/DOE sponsorship to exchange the technical knowledge and field experience as related to current information in this important field. The papers presented here reflect this effort.

  15. Second generation SLAC modulator

    SciTech Connect

    Donaldson, A.R.; Cron, J.C.; Hanselman, R.R.

    1986-06-01

    The Stanford Linear Accelerator Laboratory has undertaken the construction of a single pass electron-positron collider. In order to reach required beam energy 235 new klystrons needed upgraded modulator systems. The collider will use 50 GeV electrons and positrons. The increase in accelerator energy from the present 30 GeV necessitates the replacement of existing 35 MW klystrons with new 67 MW units. The doubling of klystron output power required a redesign of the modulator system. The 67 MW klystron needs a 350 kV beam voltage pulse with a 3.7 ..mu..s pulse width. A new pulse transformer was designed to deliver the increased voltage and pulse width. Pulse cable design was evaluated to obtain increased reliability of that critical element. The modulator, with the exception of its power supply, was rebuilt to produce the required power increase while enhancing reliability and improving maintainability. An investigation of present thyratron switch tube performance under the new operating conditions resulted in agitation and some warranted panic but these conditions were mitigated after several successful experiments and some evolutionary narrowing of the klystron pulse width. The discussion will cover the upgraded modulator system specifications and some details of the new pulse transformer tank, pulse cable, modulator, and modulator switch tube.

  16. Relationship between two Solomon Islands Earthquakes in 2007 (M8.1), 2010 (M7.1), and Seismic Gap along the Subduction Zone, Revealed by ALOS/PALSAR

    NASA Astrophysics Data System (ADS)

    Miyagi, Y.; Ozawa, T.

    2010-12-01

    The Solomon Islands are located in the southwest of the Pacific Ocean. The Australian, Woodlark, and Solomon Sea plates subduct toward the northeast beneath the Pacific plate. Interaction among these four plates cause complicated tectonics around the Solomon Islands, and have caused interplate earthquakes in the subduction zone (e.g. Lay and Kanamori, 1980; Xu and Schwarts, 1993). On April 1, 2007 (UTC), an M8.1 interplate earthquake occurred in the subduction zone between the Pacific Plate and the Australian Plate. This earthquake was accompanied by a large tsunami and caused considerable damage in the area. The Japan Aerospace Exploration Agency (JAXA) carried out emergency observations using the Phased Array type L-band Synthetic Aperture Rader (PALSAR) installed on Advanced Land Observing Satellite (ALOS), and detected more than 2m of maximum displacement using differential interferometric SAR (DInSAR) technique. Miyagi et al. (2009) estimated a slip distribution of the seismic fault mainly from the PALSAR/DInSAR data and suggested that most of a seismic gap was filled by the 2007 events, but a small seismic gap connecting to an Mw7.0-sized earthquake still remained. On January 3, 2010, an M7.1 earthquake occurred in the vicinity of the remnant seismic gap. ALOS/PALSAR observed epicentral area both before and after the event, and detected crustal deformation associated with the earthquake. We inferred fault model using the PALSAR/DInSAR data and concluded that the 2010 event was the supposed thrust earthquake filling the remnant seismic gap. A distribution of coulomb failure stress change in the epicentral area after the 2007 event suggested the possibility that the 2010 event was triggered by the 2007 earthquake.

  17. The July 2009 flurry of 3≤M<6 seismicity across Taiwan, and its possible relationship to coseismic and postseismic stresses imparted by the 1999 M=7.6 Chi-Chi earthquake

    NASA Astrophysics Data System (ADS)

    Stein, R. S.; Chan, C.

    2009-12-01

    An unusually productive sequence of twelve 3≤M<6 earthquakes occurred in central and northeastern Taiwan during 2-28 July 2009. These events have strike-slip and thrust focal mechanisms. We study the progression of these shocks, as well as smaller (M≥2) shocks since the time of the 21 September 1999 M=7.6 Chi-Chi event, and calculate of the impact of stress transferred by Chi-Chi on their occurrence. We estimate the Coulomb stress imparted by the coseismic slip, afterslip and viscoelastic deformation, all of which are important for this ramp-thrust event. Eleven out of the 12 July 2009 shocks sustained a Coulomb stress increase on at least one of their nodal planes caused by the coseismic and postseismic stress imparted by the Chi-Chi event (assuming a friction coefficient of 0.4). The stress increases range from 0.05 to 2.1 bars. Nevertheless, only 7 out of the 12 shocks can be associated with postseismic stress increases resolved onto their nodal planes, so the influence of postseismic stress remains equivocal. In examining the much more abundant M≥2 shocks, we find a good spatial correlation between the seismicity rate change and the calculated postseismic stress imparted 15 months after Chi-Chi. Here, the seismicity rate 15-75 months after the 1999 quake is divided by the rate during the 60 months before the 1999 quake. A very high rate of seismicity is observed just northeast of the northern end of the Chelungpu fault relative to the period immediately after the Chi-Chi shock. This pattern of seismicity appears to be associated with stress imparted by afterslip and viscoelastic deformation. Thus, the recent flurry of earthquakes seems largely consistent with the calculated evolution of stress and seismicity during the past decade, as does the occurrence of smaller events.

  18. "Smart" Sensor Module

    NASA Technical Reports Server (NTRS)

    Mahajan, Ajay

    2007-01-01

    An assembly that contains a sensor, sensor-signal-conditioning circuitry, a sensor-readout analog-to-digital converter (ADC), data-storage circuitry, and a microprocessor that runs special-purpose software and communicates with one or more external computer(s) has been developed as a prototype of "smart" sensor modules for monitoring the integrity and functionality (the "health") of engineering systems. Although these modules are now being designed specifically for use on rocket-engine test stands, it is anticipated that they could also readily be designed to be incorporated into health-monitoring subsystems of such diverse engineering systems as spacecraft, aircraft, land vehicles, bridges, buildings, power plants, oilrigs, and defense installations. The figure is a simplified block diagram of the "smart" sensor module. The analog sensor readout signal is processed by the ADC, the digital output of which is fed to the microprocessor. By means of a standard RS-232 cable, the microprocessor is connected to a local personal computer (PC), from which software is downloaded into a randomaccess memory in the microprocessor. The local PC is also used to debug the software. Once the software is running, the local PC is disconnected and the module is controlled by, and all output data from the module are collected by, a remote PC via an Ethernet bus. Several smart sensor modules like this one could be connected to the same Ethernet bus and controlled by the single remote PC. The software running in the microprocessor includes driver programs for operation of the sensor, programs that implement self-assessment algorithms, programs that implement protocols for communication with the external computer( s), and programs that implement evolutionary methodologies to enable the module to improve its performance over time. The design of the module and of the health-monitoring system of which it is a part reflects the understanding that the main purpose of a health

  19. Universal enveloping crossed module of Leibniz crossed modules and representations

    NASA Astrophysics Data System (ADS)

    Casado, Rafael F.; García-Martínez, Xabier; Ladra, Manuel

    2016-03-01

    The universal enveloping algebra functor UL: Lb → Alg, defined by Loday and Pirashvili [1], is extended to crossed modules. Then we construct an isomorphism between the category of representations of a Leibniz crossed module and the category of left modules over its universal enveloping crossed module of algebras. Note that the procedure followed in the proof for the Lie case cannot be adapted, since the actor in the category of Leibniz crossed modules does not always exist.

  20. Direct spatial antenna modulation

    NASA Astrophysics Data System (ADS)

    Uhl, Brecken H.

    This body of work seeks to define Direct Spatial Antenna Modulation (DSAM) as a new and unique approach to data symbol modulation and phased array control by comparing and contrasting the technique to conventional approaches. A rigorous development of the theoretical and practical implications of the DSAM technique as a general approach are presented. The theoretical development of several DSAM examples are included. Implementation and measurement results for several prototypes based on DSAM principles are analyzed. The work concludes with a summary of the impact of the present DSAM developments and a proposal for additional investigation. Results are included that show equivalent measured bit error rate performance for DSAM as compared to conventional modulation for both two-state and four-state phase modulation. Measured beam control accuracy of a DSAM phased array is included, along with several other example DSAM phased array analyses. Supported by an analysis linking a DSAM technique with complete complex-plane modulation control, the DSAM concept is applied to a commercial antenna and an experiment demonstrates wideband phase control. Analytical and simulation results demonstrate joint beamforming and modulation in a DSAM array. Several implications of the results of the investigation are important to consider: 1. The DSAM approach represents a new way to treat the conventional relationship between modulation and antennas, and has been demonstrated through a significant number and variety of analyses, simulations, and experiments. 2. The DSAM approach takes direct advantage of inherent antenna radiating properties to perform conventionally non-antenna functions; the approach is in this way both enabled and limited. 3. The DSAM approach has been shown in several examples to offer beneficial engineering performance trade-offs with respect to architecture options, as well as important performance parameters such as power consumption, breadth of frequency

  1. Strictly homogeneous laterally complete modules

    NASA Astrophysics Data System (ADS)

    Chilin, V. I.; Karimov, J. A.

    2016-03-01

    Let A be a laterally complete commutative regular algebra and X be a laterally complete A-module. In this paper we introduce a notion of homogeneous and strictly homogeneous A-modules. It is proved that any homogeneous A-module is strictly homogeneous A-module, if the Boolean algebra of all idempotents in A is multi-σ-finite.

  2. Intensity modulated proton therapy.

    PubMed

    Kooy, H M; Grassberger, C

    2015-07-01

    Intensity modulated proton therapy (IMPT) implies the electromagnetic spatial control of well-circumscribed "pencil beams" of protons of variable energy and intensity. Proton pencil beams take advantage of the charged-particle Bragg peak-the characteristic peak of dose at the end of range-combined with the modulation of pencil beam variables to create target-local modulations in dose that achieves the dose objectives. IMPT improves on X-ray intensity modulated beams (intensity modulated radiotherapy or volumetric modulated arc therapy) with dose modulation along the beam axis as well as lateral, in-field, dose modulation. The clinical practice of IMPT further improves the healthy tissue vs target dose differential in comparison with X-rays and thus allows increased target dose with dose reduction elsewhere. In addition, heavy-charged-particle beams allow for the modulation of biological effects, which is of active interest in combination with dose "painting" within a target. The clinical utilization of IMPT is actively pursued but technical, physical and clinical questions remain. Technical questions pertain to control processes for manipulating pencil beams from the creation of the proton beam to delivery within the patient within the accuracy requirement. Physical questions pertain to the interplay between the proton penetration and variations between planned and actual patient anatomical representation and the intrinsic uncertainty in tissue stopping powers (the measure of energy loss per unit distance). Clinical questions remain concerning the impact and management of the technical and physical questions within the context of the daily treatment delivery, the clinical benefit of IMPT and the biological response differential compared with X-rays against which clinical benefit will be judged. It is expected that IMPT will replace other modes of proton field delivery. Proton radiotherapy, since its first practice 50 years ago, always required the highest level of

  3. Intensity modulated proton therapy

    PubMed Central

    Grassberger, C

    2015-01-01

    Intensity modulated proton therapy (IMPT) implies the electromagnetic spatial control of well-circumscribed “pencil beams” of protons of variable energy and intensity. Proton pencil beams take advantage of the charged-particle Bragg peak—the characteristic peak of dose at the end of range—combined with the modulation of pencil beam variables to create target-local modulations in dose that achieves the dose objectives. IMPT improves on X-ray intensity modulated beams (intensity modulated radiotherapy or volumetric modulated arc therapy) with dose modulation along the beam axis as well as lateral, in-field, dose modulation. The clinical practice of IMPT further improves the healthy tissue vs target dose differential in comparison with X-rays and thus allows increased target dose with dose reduction elsewhere. In addition, heavy-charged-particle beams allow for the modulation of biological effects, which is of active interest in combination with dose “painting” within a target. The clinical utilization of IMPT is actively pursued but technical, physical and clinical questions remain. Technical questions pertain to control processes for manipulating pencil beams from the creation of the proton beam to delivery within the patient within the accuracy requirement. Physical questions pertain to the interplay between the proton penetration and variations between planned and actual patient anatomical representation and the intrinsic uncertainty in tissue stopping powers (the measure of energy loss per unit distance). Clinical questions remain concerning the impact and management of the technical and physical questions within the context of the daily treatment delivery, the clinical benefit of IMPT and the biological response differential compared with X-rays against which clinical benefit will be judged. It is expected that IMPT will replace other modes of proton field delivery. Proton radiotherapy, since its first practice 50 years ago, always required the

  4. Spatial Terahertz Modulator

    NASA Astrophysics Data System (ADS)

    Xie, Zhenwei; Wang, Xinke; Ye, Jiasheng; Feng, Shengfei; Sun, Wenfeng; Akalin, Tahsin; Zhang, Yan

    2013-11-01

    Terahertz (THz) technology is a developing and promising candidate for biological imaging, security inspection and communications, due to the low photon energy, the high transparency and the broad band properties of the THz radiation. However, a major encountered bottleneck is lack of efficient devices to manipulate the THz wave, especially to modulate the THz wave front. A wave front modulator should allow the optical or electrical control of the spatial transmission (or reflection) of an input THz wave and hence the ability to encode the information in a wave front. Here we propose a spatial THz modulator (STM) to dynamically control the THz wave front with photo-generated carriers. A computer generated THz hologram is projected onto a silicon wafer by a conventional spatial light modulator (SLM). The corresponding photo-generated carrier spatial distribution will be induced, which forms an amplitude hologram to modulate the wave front of the input THz beam. Some special intensity patterns and vortex beams are generated by using this method. This all-optical controllable STM is structure free, high resolution and broadband. It is expected to be widely used in future THz imaging and communication systems.

  5. Bunch identification module

    SciTech Connect

    Fox, J.D.

    1981-01-01

    This module provides bunch identification and timing signals for the PEP Interaction areas. Timing information is referenced to the PEP master oscillator, and adjusted in phase as a function of region. Identification signals are generated in a manner that allows observers in all interaction regions to agree on an unambiguous bunch identity. The module provides bunch identification signals via NIM level logic, upon CAMAC command, and through LED indicators. A front panel ''region select'' switch allows the same module to be used in all regions. The module has two modes of operation: a bunch identification mode and a calibration mode. In the identification mode, signals indicate which of the three bunches of electrons and positrons are interacting, and timing information about beam crossing is provided. The calibration mode is provided to assist experimenters making time of flight measurements. In the calibration mode, three distinct gating signals are referenced to a selected bunch, allowing three timing systems to be calibrated against a common standard. Physically, the bunch identifier is constructed as a single width CAMAC module. 2 figs., 1 tab.

  6. NREL module energy rating methodology

    SciTech Connect

    Whitaker, C.; Newmiller, J.; Kroposki, B.

    1995-11-01

    The goals of this project were to develop a tool for: evaluating one module in different climates; comparing different modules; provide a Q&D method for estimating periodic energy production; provide an achievable module rating; provide an incentive for manufacturers to optimize modules to non-STC conditions; and to have a consensus-based, NREL-sponsored activity. The approach taken was to simulate module energy for five reference days of various weather conditions. A performance model was developed.

  7. B4 2 After, 3D Deformation Field From Matching Pre- To Post-Event Aerial LiDAR Point Clouds, The 2010 El Mayor-Cucapah M7.2 Earthquake Case

    NASA Astrophysics Data System (ADS)

    Hinojosa-Corona, A.; Nissen, E.; Limon-Tirado, J. F.; Arrowsmith, R.; Krishnan, A.; Saripalli, S.; Oskin, M. E.; Glennie, C. L.; Arregui, S. M.; Fletcher, J. M.; Teran, O. J.

    2013-05-01

    Aerial LiDAR surveys reconstruct with amazing fidelity the sinuosity of terrain relief. In this research we explore the 3D deformation field at the surface after a big earthquake (M7.2) by comparing pre- to post-event aerial LiDAR point clouds. The April 4 2010 earthquake produced a NW-SE surface rupture ~110km long with right-lateral normal slip up to 3m in magnitude over a very favorable target: scarcely vegetated and unaltered desert mountain range, sierras El Mayor and Cucapah, in northern Baja California, close to the US-México border. It is a plate boundary region between the Pacific and North American plates. The pre-event LiDAR with lower point density (0.013-0.033 pts m-2) required filtering and post-processing before comparing with the denser (9-18 pts m-2) more accurate post event dataset. The 3D surface displacement field was determined using an adaptation of the Iterative Closest Point (ICP) algorithm, implemented in the open source Point Cloud Library (PCL). The LiDAR datasets are first split into a grid of windows, and for each one, ICP iteratively converges on the rigid body transformation (comprising translations and rotations) that best aligns the pre- to post-event points. Perturbing the pre- and post-event point clouds independently with a synthetic right lateral inverse displacements of known magnitude along a proposed fault, ICP recovered the synthetically introduced translations. Windows with dimensions of 100-200m gave the best results for datasets with these densities. The simplified surface rupture photo interpreted and mapped in the field, delineates very well the vertical displacements patterns unveiled by ICP. The method revealed block rotations, some with clockwise and others counter clockwise direction along the simplified surface rupture. As ground truth, displacements from ICP have similar values as those measured in the field along the main rupture by Fletcher and collaborators. The vertical component was better estimated than the

  8. Analysis and modelling of tsunami-induced tilt for the 2007, M = 7.6, Tocopilla and the 2010, M = 8.8 Maule earthquakes, Chile, from long-base tiltmeter and broadband seismometer records

    NASA Astrophysics Data System (ADS)

    Boudin, F.; Allgeyer, S.; Bernard, P.; Hébert, H.; Olcay, M.; Madariaga, R.; El-Madani, M.; Vilotte, J.-P.; Peyrat, S.; Nercessian, A.; Schurr, B.; Esnoult, M.-F.; Asch, G.; Nunez, I.; Kammenthaler, M.

    2013-07-01

    We present a detailed study of tsunami-induced tilt at in-land sites, to test the interest and feasibility of such analysis for tsunami detection and modelling. We studied tiltmeter and broadband seismometer records of northern Chile, detecting a clear signature of the tsunamis generated by the 2007 Tocopilla (M = 7.6) and the 2010 Maule (M = 8.8) earthquakes. We find that these records are dominated by the tilt due to the elastic loading of the oceanic floor, with a small effect of the horizontal gravitational attraction. We modelled the Maule tsunami using the seismic source model proposed by Delouis et al. and a bathymetric map, correctly fitting three tide gauge records of the area (Antofagasta, Iquique and Arica). At all the closest stations (7 STS2, 2 long-base tiltmeters), we correctly modelled the first few hours of the tilt signal for the Maule tsunami. The only phase mismatch is for the site that is closer to the ocean. We find a tilt response of 0.005-0.01 μm at 7 km away from the coastline in response to a sea level amplitude change of 10 cm. For the Maule earthquake, we observe a clear tilt signal starting 20 min before the arrival time of the tsunami at the nearest point on the coastline. This capability of tilt or seismic sensors to detect distant tsunamis before they arrive has been successfully tested with a scenario megathrust in the southern Peru-northern Chile seismic gap. However, for large events near the stations, this analysis may no longer be feasible, due to the large amplitude of the long-period seismic signals expected to obscure the loading signal. Inland tilt measurements of tsunamis smooth out short, often unmodelled wavelengths of the sea level perturbation, thus providing robust, large-scale images of the tsunami. Furthermore, tilt measurements are not expected to saturate even for the largest run-ups, nor to suffer from near-coast tsunami damages. Tiltmeters and broadband seismometers are thus valuable instruments for monitoring

  9. Deep frequency modulation interferometry.

    PubMed

    Gerberding, Oliver

    2015-06-01

    Laser interferometry with pm/Hz precision and multi-fringe dynamic range at low frequencies is a core technology to measure the motion of various objects (test masses) in space and ground based experiments for gravitational wave detection and geodesy. Even though available interferometer schemes are well understood, their construction remains complex, often involving, for example, the need to build quasi-monolithic optical benches with dozens of components. In recent years techniques have been investigated that aim to reduce this complexity by combining phase modulation techniques with sophisticated digital readout algorithms. This article presents a new scheme that uses strong laser frequency modulations in combination with the deep phase modulation readout algorithm to construct simpler and easily scalable interferometers. PMID:26072834

  10. Power module assembly

    DOEpatents

    Campbell, Jeremy B.; Newson, Steve

    2011-11-15

    A power module assembly of the type suitable for deployment in a vehicular power inverter, wherein the power inverter has a grounded chassis, is provided. The power module assembly comprises a conductive base layer electrically coupled to the chassis, an insulating layer disposed on the conductive base layer, a first conductive node disposed on the insulating layer, a second conductive node disposed on the insulating layer, wherein the first and second conductive nodes are electrically isolated from each other. The power module assembly also comprises a first capacitor having a first electrode electrically connected to the conductive base layer, and a second electrode electrically connected to the first conductive node, and further comprises a second capacitor having a first electrode electrically connected to the conductive base layer, and a second electrode electrically connected to the second conductive node.

  11. Waveform Sampler CAMAC Module

    SciTech Connect

    Freytag, D.R.; Haller, G.M.; Kang, H.; Wang, J.

    1985-09-01

    A Waveform Sampler Module (WSM) for the measurement of signal shapes coming from the multi-hit drift chambers of the SLAC SLC detector is described. The module uses a high speed, high resolution analog storage device (AMU) developed in collaboration between SLAC and Stanford University. The AMU devices together with high speed TTL clocking circuitry are packaged in a hybrid which is also suitable for mounting on the detector. The module is in CAMAC format and provides eight signal channels, each recording signal amplitude versus time in 512 cells at a sampling rate of up to 360 MHz. Data are digitized by a 12-bit ADC with a 1 ..mu..s conversion time and stored in an on-board memory accessible through CAMAC.

  12. Reliability of photovoltaic modules

    NASA Technical Reports Server (NTRS)

    Ross, R. G., Jr.

    1986-01-01

    In order to assess the reliability of photovoltaic modules, four categories of known array failure and degradation mechanisms are discussed, and target reliability allocations have been developed within each category based on the available technology and the life-cycle-cost requirements of future large-scale terrestrial applications. Cell-level failure mechanisms associated with open-circuiting or short-circuiting of individual solar cells generally arise from cell cracking or the fatigue of cell-to-cell interconnects. Power degradation mechanisms considered include gradual power loss in cells, light-induced effects, and module optical degradation. Module-level failure mechanisms and life-limiting wear-out mechanisms are also explored.

  13. Modulated infrared radiant source

    NASA Astrophysics Data System (ADS)

    Stewart, W. F.; Edwards, S. F.; Vann, D. S.; McCormick, R. F.

    1981-06-01

    A modulated, infrared radiant energy source was developed to calibrate an airborne nadir-viewing pressure modulated radiometer to be used to detect from Earth orbit trace gases in the troposphere. The technique used an 8 cm long, 0.005 cm diameter platinum-iridium wire as an isothermal, thin line radiant energy source maintained at 1200 K. A + or - 20 K signal, oscillating at controllable frequencies from dc to 20 Hz, was superimposed on it. This periodic variation of the line source energy was used to verify the pressure modulated radiometer's capability to distinguish between the signal variations caused by the Earth's background surface and the signal from the atmospheric gases of interest.

  14. Thin film module development

    NASA Technical Reports Server (NTRS)

    Jester, T.

    1985-01-01

    The design of ARCO Solar, Inc.'s Genesis G100 photovoltaic module was driven by several criteria, including environmental stability (both electrical and mechanical), consumer aesthetics, low materials costs, and manufacturing ease. The module circuitry is designed as a 12 volt battery charger, using monolithic patterning techniques on a glass superstrate. This patterning and interconnect method proves amenable to high volume, low cost production throughput, and the use of glass serves the dual role of handling ease and availability. The mechanical design of the module centers on environmental stability. Packaging of the glass superstrate circuit must provide good resistance to thermal and humidity exposure along with hi-pot insulation and hailstone impact resistance. The options considered are given. Ethylene vinyl acetate (EVA) is chosen as the pottant material for its excellent weatherability.

  15. Measuring PV module delamination

    SciTech Connect

    Murphy, E.B.

    1980-09-22

    Delamination of the encapsulating pottant from both substrate and silicon cells in solar photovoltaic modules has been a common occurrence. While the extent of delamination is in some cases minor, there are other cases where appreciably large areas have been affected. At this time, most delaminated areas do not appear to cause electrical degradation of modules; however, keeping track of delamination growth and rate of growth is important and has been difficult. More accurate measurement of delamination has been achieved by using an acoustic digitizer to record the pattern of delamination. With the aid of a computer, software can be generated that shows the exact areas of delamination. By periodic measrement of those types of modules prone to delamination, growth rates can be documented.

  16. Deep frequency modulation interferometry.

    PubMed

    Gerberding, Oliver

    2015-06-01

    Laser interferometry with pm/Hz precision and multi-fringe dynamic range at low frequencies is a core technology to measure the motion of various objects (test masses) in space and ground based experiments for gravitational wave detection and geodesy. Even though available interferometer schemes are well understood, their construction remains complex, often involving, for example, the need to build quasi-monolithic optical benches with dozens of components. In recent years techniques have been investigated that aim to reduce this complexity by combining phase modulation techniques with sophisticated digital readout algorithms. This article presents a new scheme that uses strong laser frequency modulations in combination with the deep phase modulation readout algorithm to construct simpler and easily scalable interferometers.

  17. Modulated infrared radiant source

    NASA Technical Reports Server (NTRS)

    Stewart, W. F.; Edwards, S. F.; Vann, D. S.; Mccormick, R. F.

    1981-01-01

    A modulated, infrared radiant energy source was developed to calibrate an airborne nadir-viewing pressure modulated radiometer to be used to detect from Earth orbit trace gases in the troposphere. The technique used an 8 cm long, 0.005 cm diameter platinum-iridium wire as an isothermal, thin line radiant energy source maintained at 1200 K. A + or - 20 K signal, oscillating at controllable frequencies from dc to 20 Hz, was superimposed on it. This periodic variation of the line source energy was used to verify the pressure modulated radiometer's capability to distinguish between the signal variations caused by the Earth's background surface and the signal from the atmospheric gases of interest.

  18. Space Experiment Module (SEM)

    NASA Technical Reports Server (NTRS)

    Brodell, Charles L.

    1999-01-01

    The Space Experiment Module (SEM) Program is an education initiative sponsored by the National Aeronautics and Space Administration (NASA) Shuttle Small Payloads Project. The program provides nationwide educational access to space for Kindergarten through University level students. The SEM program focuses on the science of zero-gravity and microgravity. Within the program, NASA provides small containers or "modules" for students to fly experiments on the Space Shuttle. The experiments are created, designed, built, and implemented by students with teacher and/or mentor guidance. Student experiment modules are flown in a "carrier" which resides in the cargo bay of the Space Shuttle. The carrier supplies power to, and the means to control and collect data from each experiment.

  19. Quantitative velocity modulation spectroscopy

    NASA Astrophysics Data System (ADS)

    Hodges, James N.; McCall, Benjamin J.

    2016-05-01

    Velocity Modulation Spectroscopy (VMS) is arguably the most important development in the 20th century for spectroscopic study of molecular ions. For decades, interpretation of VMS lineshapes has presented challenges due to the intrinsic covariance of fit parameters including velocity modulation amplitude, linewidth, and intensity. This limitation has stifled the growth of this technique into the quantitative realm. In this work, we show that subtle changes in the lineshape can be used to help address this complexity. This allows for determination of the linewidth, intensity relative to other transitions, velocity modulation amplitude, and electric field strength in the positive column of a glow discharge. Additionally, we explain the large homogeneous component of the linewidth that has been previously described. Using this component, the ion mobility can be determined.

  20. Printed Module Interconnects

    SciTech Connect

    Stockert, Talysa R.; Fields, Jeremy D.; Pach, Gregory F.; Mauger, Scott A.; van Hest, Maikel F. A. M.

    2015-06-14

    Monolithic interconnects in photovoltaic modules connect adjacent cells in series, and are typically formed sequentially involving multiple deposition and scribing steps. Interconnect widths of 500 um every 10 mm result in 5% dead area, which does not contribute to power generation in an interconnected solar panel. This work expands on previous work that introduced an alternative interconnection method capable of producing interconnect widths less than 100 um. The interconnect is added to the module in a single step after deposition of the photovoltaic stack, eliminating the need for scribe alignment. This alternative method can be used for all types of thin film photovoltaic modules. Voltage addition with copper-indium-gallium-diselenide (CIGS) solar cells using a 2-scribe printed interconnect approach is demonstrated. Additionally, interconnect widths of 250 um are shown.

  1. Thin film module development

    NASA Astrophysics Data System (ADS)

    Jester, T.

    1985-10-01

    The design of ARCO Solar, Inc.'s Genesis G100 photovoltaic module was driven by several criteria, including environmental stability (both electrical and mechanical), consumer aesthetics, low materials costs, and manufacturing ease. The module circuitry is designed as a 12 volt battery charger, using monolithic patterning techniques on a glass superstrate. This patterning and interconnect method proves amenable to high volume, low cost production throughput, and the use of glass serves the dual role of handling ease and availability. The mechanical design of the module centers on environmental stability. Packaging of the glass superstrate circuit must provide good resistance to thermal and humidity exposure along with hi-pot insulation and hailstone impact resistance. The options considered are given. Ethylene vinyl acetate (EVA) is chosen as the pottant material for its excellent weatherability.

  2. Flexible programmable logic module

    DOEpatents

    Robertson, Perry J.; Hutchinson, Robert L.; Pierson, Lyndon G.

    2001-01-01

    The circuit module of this invention is a VME board containing a plurality of programmable logic devices (PLDs), a controlled impedance clock tree, and interconnecting buses. The PLDs are arranged to permit systolic processing of a problem by offering wide data buses and a plurality of processing nodes. The board contains a clock reference and clock distribution tree that can drive each of the PLDs with two critically timed clock references. External clock references can be used to drive additional circuit modules all operating from the same synchronous clock reference.

  3. Photovoltaic module and interlocked stack of photovoltaic modules

    DOEpatents

    Wares, Brian S.

    2012-09-04

    One embodiment relates to an arrangement of photovoltaic modules configured for transportation. The arrangement includes a plurality of photovoltaic modules, each photovoltaic module including a frame having at least a top member and a bottom member. A plurality of alignment features are included on the top member of each frame, and a plurality of alignment features are included on the bottom member of each frame. Adjacent photovoltaic modules are interlocked by the alignment features on the top member of a lower module fitting together with the alignment features on the bottom member of an upper module. Other embodiments, features and aspects are also disclosed.

  4. Drupal Contributed Modules

    SciTech Connect

    Fries, Samuel B.; French, Shelane

    2014-10-01

    These Drupal Modules extend the functionality of Drupal by including specific styles for dates and tabs, publishing options for scheduled and immediate publication of content modes, field visibility in content forms, keyword block filters (taxonomy based), adding content nodes to a specified queue for display in views, and status display of workflow settings.

  5. Rescue Manual. Module 8.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Instructional Materials Lab.

    This learner manual for rescuers covers the current techniques or practices required in the rescue service. The eighth of 10 modules contains 6 chapters: (1) trench rescue; (2) shoring and tunneling techniques; (3) farm accident rescue; (4) wilderness search and rescue; (5) aircraft rescue; and (6) helicopter information. Key points, an…

  6. Coast Guard Firefighting Module

    NASA Technical Reports Server (NTRS)

    1977-01-01

    NASA and the U.S. Coast Guard are jointly developing a lightweight, helicopter-transportable, completely self-contained firefighting module for combating shipboard and dockside fires. The project draws upon NASA technology in high-capacity rocket engine pumps, lightweight materials and compact packaging.

  7. Rescue Manual. Module 4.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Instructional Materials Lab.

    This learner manual for rescuers covers the current techniques or practices required in the rescue service. The fourth of 10 modules contains 8 chapters: (1) construction and characteristics of rescue rope; (2) knots, bends, and hitches; (3) critical angles; (4) raising systems; (5) rigging; (6) using the brake-bar rack for rope rescue; (7) rope…

  8. Formed photovoltaic module busbars

    DOEpatents

    Rose, Douglas; Daroczi, Shan; Phu, Thomas

    2015-11-10

    A cell connection piece for a photovoltaic module is disclosed herein. The cell connection piece includes an interconnect bus, a plurality of bus tabs unitarily formed with the interconnect bus, and a terminal bus coupled with the interconnect bus. The plurality of bus tabs extend from the interconnect bus. The terminal bus includes a non-linear portion.

  9. Paratransit: An Instructional Module.

    ERIC Educational Resources Information Center

    Scalici, Anthony

    A concept-based introduction to paratransit is provided in this instructional module for undergraduate and graduate transportation-related courses for disciplines such as engineering, business, marketing, and technology. The concept of paratransit generally refers to modes of transportation other than mass transit and solo-driven automobiles. The…

  10. An Integrated Teaching Module.

    ERIC Educational Resources Information Center

    Samuel, Marie R.; Seiferth, Berniece B.

    This integrated teaching module provides elementary and junior high school teachers with a "hands-on" approach to studying the Anasazi Indian. Emphasis is on creative exploration that focuses on integrating art, music, poetry, writing, geography, dance, history, anthropology, sociology, and archaeology. Replicas of artifacts, contemporary Indian…

  11. Behavior Management: Training Module.

    ERIC Educational Resources Information Center

    Glen, Sally; McCoy, Youlonda

    This publication, the third in a series of modules designed for paraprofessionals working with handicapped children, presents objectives and related activities for three competencies in behavior management. The first competency, on the definition and underlying concepts of behavior management, focuses on the application of behavior management…

  12. Rescue Manual. Module 6.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Instructional Materials Lab.

    This learner manual for rescuers covers the current techniques or practices required in the rescue service. The sixth of 10 modules contains 4 chapters: (1) industrial rescue; (2) rescue from a confined space; (3) extrication from heavy equipment; and (4) rescue operations involving elevators. Key points, an introduction, and conclusion accompany…

  13. Coplanar interconnection module

    NASA Technical Reports Server (NTRS)

    Steward, R. D.; Windsor, H. F.

    1970-01-01

    Module for interconnecting a semiconductor array to external leads or components incorporates a metal external heat sink for cooling the array. Heat sink, extending down from the molded block that supports the array, is immersed in a liquid nitrogen bath which is designed to maintain the desired array temperature.

  14. Rescue Manual. Module 9.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Instructional Materials Lab.

    This learner manual for rescuers covers the current techniques or practices required in the rescue service. The ninth of 10 modules contains 7 chapters: (1) ice characteristics; (2) river characteristics and tactics for rescue; (3) water rescue techniques; (4) water rescue/recovery operations; (5) dive operations; (6) water rescue equipment; and…

  15. Packaging of electronic modules

    NASA Technical Reports Server (NTRS)

    Katzin, L.

    1966-01-01

    Study of design approaches that are taken toward optimizing the packaging of electronic modules with respect to size, shape, component orientation, interconnections, and structural support. The study does not present a solution to specific packaging problems, but rather the factors to be considered to achieve optimum packaging designs.

  16. SPACE: Intermediate Level Modules.

    ERIC Educational Resources Information Center

    Indiana State Dept. of Education, Indianapolis. Center for School Improvement and Performance.

    These modules were developed to assist teachers at the intermediate level to move away from extensive skill practice and toward more meaningful interdisciplinary learning. This packet, to be used by teachers in the summer Extended Learning Program, provides detailed thematic lesson plans matched to the Indiana Curriculum Proficiency Guide. The…

  17. Product Module Rig Test

    NASA Technical Reports Server (NTRS)

    Holdeman, James D. (Technical Monitor); Chiappetta, Louis, Jr.; Hautman, Donald J.; Ols, John T.; Padget, Frederick C., IV; Peschke, William O. T.; Shirley, John A.; Siskind, Kenneth S.

    2004-01-01

    The low emissions potential of a Rich-Quench-Lean (RQL) combustor for use in the High Speed Civil Transport (HSCT) application was evaluated as part of Work Breakdown Structure (WBS) 1.0.2.7 of the NASA Critical Propulsion Components (CPC) Program under Contract NAS3-27235. Combustion testing was conducted in cell 1E of the Jet Burner Test Stand at United Technologies Research Center. Specifically, a Rich-Quench-Lean combustor, utilizing reduced scale quench technology implemented in a quench vane concept in a product-like configuration (Product Module Rig), demonstrated the capability of achieving an emissions index of nitrogen oxides (NOx EI) of 8.5 gm/Kg fuel at the supersonic flight condition (relative to the program goal of 5 gm/Kg fuel). Developmental parametric testing of various quench vane configurations in the more fundamental flametube, Single Module Rig Configuration, demonstrated NOx EI as low as 5.2. All configurations in both the Product Module Rig configuration and the Single Module Rig configuration demonstrated exceptional efficiencies, greater than 99.95 percent, relative to the program goal of 99.9 percent efficiency at supersonic cruise conditions. Sensitivity of emissions to quench orifice design parameters were determined during the parametric quench vane test series in support of the design of the Product Module Rig configuration. For the rectangular quench orifices investigated, an aspect ratio (length/width) of approximately 2 was found to be near optimum. An optimum for orifice spacing was found to exist at approximately 0.167 inches, resulting in 24 orifices per side of a quench vane, for the 0.435 inch quench zone channel height investigated in the Single Module Rig. Smaller quench zone channel heights appeared to be beneficial in reducing emissions. Measurements were also obtained in the Single Module Rig configuration on the sensitivity of emissions to the critical combustor parameters of fuel/air ratio, pressure drop, and residence

  18. Method of monolithic module assembly

    DOEpatents

    Gee, James M.; Garrett, Stephen E.; Morgan, William P.; Worobey, Walter

    1999-01-01

    Methods for "monolithic module assembly" which translate many of the advantages of monolithic module construction of thin-film PV modules to wafered c-Si PV modules. Methods employ using back-contact solar cells positioned atop electrically conductive circuit elements affixed to a planar support so that a circuit capable of generating electric power is created. The modules are encapsulated using encapsulant materials such as EVA which are commonly used in photovoltaic module manufacture. The methods of the invention allow multiple cells to be electrically connected in a single encapsulation step rather than by sequential soldering which characterizes the currently used commercial practices.

  19. Coseismic Deformations Associated with the M=7.2, April 04, 2010, El Mayor-Cucapah Earthquake, Observed from Leveling Survey, Geotechnical Instruments and Water Level Changes in the Mexicali Valley

    NASA Astrophysics Data System (ADS)

    Glowacka, E.; Robles, B.; Vázquez, R.; Sarychikhina, O.; Suárez-Vidal, F.; Ramirez, J.; Nava Pichardo, F. A.; Farfan, F.; Diaz de Cossio, G.

    2010-12-01

    A first order, second class leveling survey in the Mexicali Valley had been just finished in February 2010, for a project carried out by CICESE (Center for Scientific Research and Higher Education of Ensenada), IMTA (Mexican Institute of Water Technology) and CONAGUA (National Water Comission). Immediately after the M=7.2 earthquake the survey was repeated along 240 km of the profiles in the area of the Cerro Prieto pull-apart basin. The leveling started at the LN00 GPS monument in La Puerta. Overall, an uplift of about 30 cm towards the NE, along the 38 km line, in direction SW-NE is observed with larger gradient to the South of the area. Three subsidence bowls differ from this general pattern. One, south from Ejido Saltillo, with the relative subsidence of 19 cm (considering the displacement at LN00 as zero subsidence), probably reflects subsidence of the Saltillo-Guerrero graben; the second, with a subsidence of 23 cm, is situated south from Ejido Nuevo Leon and can be related to the subsidence triggered by the earthquake in the production area of Cerro Prieto IV. For the third one, with relative depth of 36 cm, situated close to Zacamoto, the southeastern limit cannot be determined, so only a comparison with other methods can explain the origin of this anomaly. All the subsidence bowls are associated with liquefaction observed in the area, with more liquefaction observed close to Zacamoto. Since 1996, CICESE has been operating a network of geotechnical instruments (REDECVAM) for continuous recording of deformation related to tectonic (seismic and interseismic) phenomena, as well as anthropogenic deformation caused by the deep fluid extraction at the Cerro Prieto Geothermal Field. The instruments are installed along the faults which limit the Cerro Prieto pull-apart basin at a distance from 8 to 15 km from the epicenter. Coseismic step-like groundwater level changes ranging from 0.4 to 5.0 meters were recorded at 4 wells in the Cerro Prieto Pull apart

  20. Axially Modulated Plasma Waveguides

    SciTech Connect

    Layer, B. D.; York, A. G.; Varma, S.; Chen, Y.-H.; Milchberg, H. M.

    2009-01-22

    We demonstrate two techniques for making periodically modulated plasma waveguides-one with sharp, stable voids as short as 50 {mu}m with a period as small as 200 {mu}m, and another which modulates the waveguide diameter with a corrugation period as short as 35 {mu}m[1]. These features persist as the plasma expands for the full lifetime of the waveguide (>6 ns). The waveguides were made using the hydrodynamic shock method in a cluster jet using hydrogen, nitrogen, and argon. We demonstrate guided propagation at intensities up to 2x10{sup 17} W/cm{sup 2}, limited by our laser energy currently available. This technique is useful for quasi-phase matching to allow efficient coupling of laser energy to acceleration of relativistic electrons or generation of coherent electromagnetic radiation at selected frequencies.

  1. Vapor compression distillation module

    NASA Technical Reports Server (NTRS)

    Nuccio, P. P.

    1975-01-01

    A Vapor Compression Distillation (VCD) module was developed and evaluated as part of a Space Station Prototype (SSP) environmental control and life support system. The VCD module includes the waste tankage, pumps, post-treatment cells, automatic controls and fault detection instrumentation. Development problems were encountered with two components: the liquid pumps, and the waste tank and quantity gauge. Peristaltic pumps were selected instead of gear pumps, and a sub-program of materials and design optimization was undertaken leading to a projected life greater than 10,000 hours of continuous operation. A bladder tank was designed and built to contain the waste liquids and deliver it to the processor. A detrimental pressure pattern imposed upon the bladder by a force-operated quantity gauge was corrected by rearranging the force application, and design goals were achieved. System testing has demonstrated that all performance goals have been fulfilled.

  2. VERDE Analytic Modules

    2008-01-15

    The Verde Analytic Modules permit the user to ingest openly available data feeds about phenomenology (storm tracks, wind, precipitation, earthquake, wildfires, and similar natural and manmade power grid disruptions and forecast power outages, restoration times, customers outaged, and key facilities that will lose power. Damage areas are predicted using historic damage criteria of the affected area. The modules use a cellular automata approach to estimating the distribution circuits assigned to geo-located substations. Population estimates servedmore » within the service areas are located within 1 km grid cells and converted to customer counts by conversion through demographic estimation of households and commercial firms within the population cells. Restoration times are estimated by agent-based simulation of restoration crews working according to utility published prioritization calibrated by historic performance.« less

  3. Modulators of decision making.

    PubMed

    Doya, Kenji

    2008-04-01

    Human and animal decisions are modulated by a variety of environmental and intrinsic contexts. Here I consider computational factors that can affect decision making and review anatomical structures and neurochemical systems that are related to contextual modulation of decision making. Expectation of a high reward can motivate a subject to go for an action despite a large cost, a decision that is influenced by dopamine in the anterior cingulate cortex. Uncertainty of action outcomes can promote risk taking and exploratory choices, in which norepinephrine and the orbitofrontal cortex appear to be involved. Predictable environments should facilitate consideration of longer-delayed rewards, which depends on serotonin in the dorsal striatum and dorsal prefrontal cortex. This article aims to sort out factors that affect the process of decision making from the viewpoint of reinforcement learning theory and to bridge between such computational needs and their neurophysiological substrates.

  4. Modulated Elliptical Slot

    NASA Technical Reports Server (NTRS)

    Abou-Khousa, M. A.

    2009-01-01

    A novel modulated slot design has been proposed and tested. The proposed slot is aimed to replace the inefficient small dipoles used in conventional MST-based imaging systems. The developed slot is very attractive as MST array element due to its small size and high efficiency/modulation depth. In fact, the developed slot has been successfully used to implement the first prototype of a microwave camera operating at 24 GHZ. It is also being used in the design of the second generation of the camera. Finally, the designed elliptical slot can be used as an electronically controlled waveguide iris for many other purposes (for instance in constructing waveguide reflective phase shifters and multiplexers/switches).

  5. Parabolic dish module experiment

    NASA Astrophysics Data System (ADS)

    1986-03-01

    A development test model of the 8-meter Solar Brayton Parabolic Dish Module has been designed, fabricated, and tested. The test model consists of five major subsystems: Sanders ceramic honeycomb solar receiver; LaJet LEC460 solar concentrator; AiRsearch SABC MKIIIA engine, Abacus 8 kW ac inverter; and a Sanders designed and built system controller. Goals of the tests were to integrate subsystem components into a working module, demonstrate the concept, and generate 5 kWe (hybrid) and 4.7 kWe (solar only) input. All subsystem integration goals were successfully achieved, but system performance efficiency was lower than expected. Contributing causes of the lower performance efficiencies have been identified. Modifications needed to restore performance to the required levels and improve the system life cycle cost have been addressed and are the subject of this final report.

  6. Lightweight Trauma Module - LTM

    NASA Technical Reports Server (NTRS)

    Hatfield, Thomas

    2008-01-01

    Current patient movement items (PMI) supporting the military's Critical Care Air Transport Team (CCATT) mission as well as the Crew Health Care System for space (CHeCS) have significant limitations: size, weight, battery duration, and dated clinical technology. The LTM is a small, 20 lb., system integrating diagnostic and therapeutic clinical capabilities along with onboard data management, communication services and automated care algorithms to meet new Aeromedical Evacuation requirements. The Lightweight Trauma Module is an Impact Instrumentation, Inc. project with strong Industry, DoD, NASA, and Academia partnerships aimed at developing the next generation of smart and rugged critical care tools for hazardous environments ranging from the battlefield to space exploration. The LTM is a combination ventilator/critical care monitor/therapeutic system with integrated automatic control systems. Additional capabilities are provided with small external modules.

  7. VERDE Analytic Modules

    SciTech Connect

    2008-01-15

    The Verde Analytic Modules permit the user to ingest openly available data feeds about phenomenology (storm tracks, wind, precipitation, earthquake, wildfires, and similar natural and manmade power grid disruptions and forecast power outages, restoration times, customers outaged, and key facilities that will lose power. Damage areas are predicted using historic damage criteria of the affected area. The modules use a cellular automata approach to estimating the distribution circuits assigned to geo-located substations. Population estimates served within the service areas are located within 1 km grid cells and converted to customer counts by conversion through demographic estimation of households and commercial firms within the population cells. Restoration times are estimated by agent-based simulation of restoration crews working according to utility published prioritization calibrated by historic performance.

  8. 30% CPV Module Milestone

    NASA Astrophysics Data System (ADS)

    Gordon, Robert; Kinsey, Geoff; Nayaak, Adi; Garboushian, Vahan

    2010-10-01

    Concentrating Photovoltaics has held out the promise of low cost solar electricity for now several decades. Steady progress towards this goal in the 80's and 90's gradually produced more efficient and reliable systems. System efficiency is regarded as the largest factor in lowering the electricity cost and the relatively recent advent of the terrestrial multi-junction solar cell has pressed this race forward dramatically. CPV systems are now exhibiting impressive AC field efficiencies of 25% and more, approximately twice that of the best flat plate systems available today. Amonix inc. has just tested their latest generation multi-junction module design, achieving over 31% DC efficiency at near PVUSA test conditions. Inculcating this design into their next MegaModule is forthcoming, but the expected AC system field efficiency should be significantly higher than current 25% levels.

  9. Module isolation devices

    DOEpatents

    Carolan, Michael Francis; Cooke, John Albert; Buzinski, Michael David

    2010-04-27

    A gas flow isolation device includes a gas flow isolation valve movable from an opened condition to a closed condition. The module isolation valve in one embodiment includes a rupture disk in flow communication with a flow of gas when the module isolation valve is in an opened condition. The rupture disk ruptures when a predetermined pressure differential occurs across it causing the isolation valve to close. In one embodiment the valve is mechanically linked to the rupture disk to maintain the valve in an opened condition when the rupture disk is intact, and which permits the valve to move into a closed condition when the rupture disk ruptures. In another embodiment a crushable member maintains the valve in an open condition, and the flow of gas passed the valve upon rupturing of the rupture disk compresses the crushable member to close the isolation valve.

  10. Stable local oscillator module.

    SciTech Connect

    Brocato, Robert Wesley

    2007-11-01

    This report gives a description of the development of a Stable Local Oscillator (StaLO) multi-chip module (MCM). It is a follow-on report to SAND2006-6414, Stable Local Oscillator Microcircuit. The StaLO accepts a 100MHz input signal and produces output signals at 1.2, 3.3, and 3.6 GHz. The circuit is built as a multi-chip module (MCM), since it makes use of integrated circuit technologies in silicon and lithium niobate as well as discrete passive components. This report describes the development of an MCM-based version of the complete StaLO, fabricated on an alumina thick film hybrid substrate.

  11. Thermal modulation for gas chromatography

    NASA Technical Reports Server (NTRS)

    Hasselbrink, Ernest F. (Inventor); Libardoni, Mark (Inventor); Stewart, Kristine (Inventor); Waite, J. Hunter (Inventor); Block, Bruce P. (Inventor); Sacks, Richard D. (Inventor)

    2007-01-01

    A thermal modulator device for gas chromatography and associated methods. The thermal modulator device includes a recirculating fluid cooling member, an electrically conductive capillary in direct thermal contact with the cooling member, and a power supply electrically coupled to the capillary and operable for controlled resistive heating of the capillary. The capillary can include more than one separate thermally modulated sections.

  12. Headstart German Program. Module 5.

    ERIC Educational Resources Information Center

    Defense Language Inst., Monterey, CA.

    This is the fifth module of 10 in the German Headstart program. Each of the 3 units in the module contains objectives, exercises, and a self-evaluation quiz. In addition, there are several supplementary exercises and self-evaluations. The objective of this module is to enable the student to use and understand: (1) courtesy expressions; (2) time…

  13. Headstart German Program. Module 9.

    ERIC Educational Resources Information Center

    Defense Language Inst., Monterey, CA.

    This is the ninth module of 10 in the German Headstart program. The single unit in the module contains objectives, exercises, and a self-evaluation quiz. In addition, there are several supplementary exercises and self-evaluations. The objective of this module is to familiarize the student with some of the terminology used by military personnel in…

  14. Assessing Current Status. Module 4.

    ERIC Educational Resources Information Center

    DuBois, Phyllis

    This staff development module is part of one of three groups of career guidance modules developed, field-tested and revised by a six-state consortium coordinated by the American Institutes for Research. This module is the fourth in a series on developing a comprehensive career guidance program at the high school level, designed to aid guidance…

  15. Headstart German Program. Module 7.

    ERIC Educational Resources Information Center

    Defense Language Inst., Monterey, CA.

    This is the seventh module of 10 in the German Headstart program. Each of the 2 units in the module contains objectives, exercises, and a self-evaluation quiz. In addition, there are several supplementary exercises and self-evaluations. The objective of this module is to enable the student to use all the services of a German post-office, such as…

  16. Modulation instability: The beginning

    NASA Astrophysics Data System (ADS)

    Zakharov, V. E.; Ostrovsky, L. A.

    2009-03-01

    We discuss the early history of an important field of “sturm and drang” in modern theory of nonlinear waves. It is demonstrated how scientific demand resulted in independent and almost simultaneous publications by many different authors on modulation instability, a phenomenon resulting in a variety of nonlinear processes such as envelope solitons, envelope shocks, freak waves, etc. Examples from water wave hydrodynamics, electrodynamics, nonlinear optics, and convection theory are given.

  17. Tandem resonator reflectance modulator

    DOEpatents

    Fritz, I.J.; Wendt, J.R.

    1994-09-06

    A wide band optical modulator is grown on a substrate as tandem Fabry-Perot resonators including three mirrors spaced by two cavities. The absorption of one cavity is changed relative to the absorption of the other cavity by an applied electric field, to cause a change in total reflected light, as light reflecting from the outer mirrors is in phase and light reflecting from the inner mirror is out of phase with light from the outer mirrors. 8 figs.

  18. Tandem resonator reflectance modulator

    DOEpatents

    Fritz, Ian J.; Wendt, Joel R.

    1994-01-01

    A wide band optical modulator is grown on a substrate as tandem Fabry-Perot resonators including three mirrors spaced by two cavities. The absorption of one cavity is changed relative to the absorption of the other cavity by an applied electric field, to cause a change in total reflected light, as light reflecting from the outer mirrors is in phase and light reflecting from the inner mirror is out of phase with light from the outer mirrors.

  19. Packaging the MAMA module

    NASA Astrophysics Data System (ADS)

    Seals, J. Dennis

    1994-10-01

    The MAMA (Mixed Arithmetic, Multiprocessing Array) module is being developed to evaluate new packaging technologies and processing paradigms for advanced military processing systems. The architecture supports a tight mix of signal, data,and I/O processing at GFLOP throughput rates. It is fabricated using only commercial-on-the-sehlf (COTS) chips and will provide a high level of durability. Its attributes are largely the result of two new interconnection and packaging technologies. Chip-in-board packaging is used to reduce local x-y communication delays and solder joints, while significantly improving board-level packaging density. A unique 3-D interconnection technology called a cross-over cell has been developed to reduce board-to-board communication delays, drive power, glue logic, and card-edge pin-outs. These technologies enable true 3-D structures that are form, fit and connector compatible with conventional line-replacable modules. The module's design rational, packaging technology, and basic architecture will be presented in this paper.

  20. Allosteric modulation of caspases.

    PubMed

    Häcker, Hans-Georg; Sisay, Mihiret Tekeste; Gütschow, Michael

    2011-11-01

    Caspases are proteolytic enzymes mainly involved in the induction and execution phases of apoptosis. This type of programmed cell death is an essential regulatory process required to maintain the integrity and homeostasis of multicellular organisms. Inappropriate apoptosis is attributed a key role in many human diseases, including neurodegenerative disorders, ischemic damage, autoimmune diseases and cancer. Allosteric modulation of the function of a protein occurs when the regulatory trigger, such as the binding of a small effector or inhibitor molecule, takes place some distance from the protein's active site. In recent years, several caspases have been identified that possess allosteric sites and binding of small molecule to these sites resulted in the modulation of enzyme activities. Regulation of caspase activity by small molecule allosteric modulators is believed to be of great therapeutic importance. In this review we give brief highlights on recent developments in identifying and characterizing natural and synthetic allosteric inhibitors as well as activators of caspases and discuss their potential in drug discovery and protein engineering. PMID:21807025

  1. Receiver Gain Modulation Circuit

    NASA Technical Reports Server (NTRS)

    Jones, Hollis; Racette, Paul; Walker, David; Gu, Dazhen

    2011-01-01

    A receiver gain modulation circuit (RGMC) was developed that modulates the power gain of the output of a radiometer receiver with a test signal. As the radiometer receiver switches between calibration noise references, the test signal is mixed with the calibrated noise and thus produces an ensemble set of measurements from which ensemble statistical analysis can be used to extract statistical information about the test signal. The RGMC is an enabling technology of the ensemble detector. As a key component for achieving ensemble detection and analysis, the RGMC has broad aeronautical and space applications. The RGMC can be used to test and develop new calibration algorithms, for example, to detect gain anomalies, and/or correct for slow drifts that affect climate-quality measurements over an accelerated time scale. A generalized approach to analyzing radiometer system designs yields a mathematical treatment of noise reference measurements in calibration algorithms. By treating the measurements from the different noise references as ensemble samples of the receiver state, i.e. receiver gain, a quantitative description of the non-stationary properties of the underlying receiver fluctuations can be derived. Excellent agreement has been obtained between model calculations and radiometric measurements. The mathematical formulation is equivalent to modulating the gain of a stable receiver with an externally generated signal and is the basis for ensemble detection and analysis (EDA). The concept of generating ensemble data sets using an ensemble detector is similar to the ensemble data sets generated as part of ensemble empirical mode decomposition (EEMD) with exception of a key distinguishing factor. EEMD adds noise to the signal under study whereas EDA mixes the signal with calibrated noise. It is mixing with calibrated noise that permits the measurement of temporal-functional variability of uncertainty in the underlying process. The RGMC permits the evaluation of EDA by

  2. AC photovoltaic module magnetic fields

    SciTech Connect

    Jennings, C.; Chang, G.J.; Reyes, A.B.; Whitaker, C.M.

    1997-12-31

    Implementation of alternating current (AC) photovoltaic (PV) modules, particularly for distributed applications such as PV rooftops and facades, may be slowed by public concern about electric and magnetic fields (EMF). This paper documents magnetic field measurements on an AC PV module, complementing EMF research on direct-current PV modules conducted by PG and E in 1993. Although not comprehensive, the PV EMF data indicate that 60 Hz magnetic fields (the EMF type of greatest public concern) from PV modules are comparable to, or significantly less than, those from household appliances. Given the present EMF research knowledge, AC PV module EMF may not merit considerable concern.

  3. Accumulate Repeat Accumulate Coded Modulation

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative coded modulation scheme called 'Accumulate Repeat Accumulate Coded Modulation' (ARA coded modulation). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes that are combined with high level modulation. Thus at the decoder belief propagation can be used for iterative decoding of ARA coded modulation on a graph, provided a demapper transforms the received in-phase and quadrature samples to reliability of the bits.

  4. Effects of modulation phase of ultrasound-modulated light on the ultrasound-modulated optical image in turbid media.

    PubMed

    Weng, Cuncheng; Zhang, Jing

    2012-04-01

    In this paper, our investigations suggest that the modulation phase of ultrasound-modulated light escaping from the different locations in the ultrasonic field is different. In turbid media, the modulation phase causes the ultrasound-modulated light intensity collected outside the media to fluctuate. However, the ultrasound-modulated optical technology uses the ultrasound-modulated light signals to image. Consequently, the modulation phase affects the quality of ultrasound-modulated optical imaging.

  5. On staggered indecomposable Virasoro modules

    NASA Astrophysics Data System (ADS)

    Kytölä, Kalle; Ridout, David

    2009-12-01

    In this article, certain indecomposable Virasoro modules are studied. Specifically, the Virasoro mode L0 is assumed to be nondiagonalizable, possessing Jordan blocks of rank 2. Moreover, the module is further assumed to have a highest weight submodule, the "left module," and that the quotient by this submodule yields another highest weight module, the "right module." Such modules, which have been called staggered, have appeared repeatedly in the logarithmic conformal field theory literature, but their theory has not been explored in full generality. Here, such a theory is developed for the Virasoro algebra using rather elementary techniques. The focus centers on two different but related questions typically encountered in practical studies: How can one identify a given staggered module, and how can one demonstrate the existence of a proposed staggered module. Given just the values of the highest weights of the left and right modules, themselves subject to simple necessary conditions, invariants are defined which together with the knowledge of the left and right modules uniquely identify a staggered module. The possible values of these invariants form a vector space of dimension 0, 1, or 2, and the structures of the left and right modules limit the isomorphism classes of the corresponding staggered modules to an affine subspace (possibly empty). The number of invariants and affine restrictions is purely determined by the structures of the left and right modules. Moreover, in order to facilitate applications, the expressions for the invariants and restrictions are given by formulas as explicit as possible (they generally rely on expressions for Virasoro singular vectors). Finally, the text is liberally peppered throughout with examples illustrating the general concepts. These have been carefully chosen for their physical relevance or for the novel features they exhibit.

  6. Managing Medical Logic Modules.

    PubMed Central

    Aguirre, A. R.; Roderer, N. K.

    1991-01-01

    A key element of IAIMS development at the Columbia Presbyterian Medical Center (CPMC) is the Medical Logic Module (MLM), designed to provide decision support to clinical users. A standard has been established for MLMs, and a number of institutions have agreed in principle to share them. At CPMC, MLMs are under development and MLMs from other institutions are being reviewed. The Columbia Health Sciences Library has developed a management system for MLMs which supports both internal development and sharing of MLMs among institutions. This paper describes the elements of the MLM management system. PMID:1807599

  7. Exothermic furnace module

    NASA Technical Reports Server (NTRS)

    Poorman, R. M. (Inventor)

    1982-01-01

    An exothermic furnace module is disclosed for processing materials in space which includes an insulated casing and a sample support, carried within the casing which supports a sample container. An exothermic heat source includes a plurality of segments of exothermic material stacked one upon another to produce a desired temperature profile when ignited. The exothermic material segments are constructed in the form of an annular element having a recess opening which defines an open central core throughout the vertical axis of the stacked exothermic material. The sample container is arranged within the core of the stacked exothermic heating material.

  8. Modulation of whistlers

    NASA Astrophysics Data System (ADS)

    Sivokon', V. P.; Bogdanov, V. V.; Druzhin, G. I.; Cherneva, N. V.; Kubyshkin, A. V.; Sannikov, D. V.; Agranat, I. V.

    2014-11-01

    Analysis of the experimental data obtained at Paratunka observatory (53.02° N, 158.65° E; L = 2.3) has revealed a nonstandard form of whistlers involving spectral lines that are symmetric with respect to the whistler. We have shown that this form is most likely due to the amplitude modulation of whistlers by electromagnetic pulses with a length of around 1 s and carrier frequency of around 1.1 kHz. We have suggested that these pulses could be emitted by the auroral electrojet modified by heating radiation from the HAARP facility (62.30° N, 145.30° W; L > 4.2).

  9. TASMANIAN Sparse Grids Module

    SciTech Connect

    and Drayton Munster, Miroslav Stoyanov

    2013-09-20

    Sparse Grids are the family of methods of choice for multidimensional integration and interpolation in low to moderate number of dimensions. The method is to select extend a one dimensional set of abscissas, weights and basis functions by taking a subset of all possible tensor products. The module provides the ability to create global and local approximations based on polynomials and wavelets. The software has three components, a library, a wrapper for the library that provides a command line interface via text files ad a MATLAB interface via the command line tool.

  10. TASMANIAN Sparse Grids Module

    2013-09-20

    Sparse Grids are the family of methods of choice for multidimensional integration and interpolation in low to moderate number of dimensions. The method is to select extend a one dimensional set of abscissas, weights and basis functions by taking a subset of all possible tensor products. The module provides the ability to create global and local approximations based on polynomials and wavelets. The software has three components, a library, a wrapper for the library thatmore » provides a command line interface via text files ad a MATLAB interface via the command line tool.« less

  11. Perfusion Bioreactor Module

    NASA Technical Reports Server (NTRS)

    Morrison, Dennis R.

    1990-01-01

    Perfusion bioreactor module, self-contained, closed-loop cell-culture system that operates in microgravity or on Earth. Equipment supports growth or long-term maintenance of cultures of human or other fragile cells for experiments in basic cell biology or process technology. Designed to support proliferation (initially at exponential rates of growth) of cells in complex growth medium and to maintain confluent cells in defined medium under conditions optimized to permit or encourage selected functions of cells, including secretion of products of cells into medium.

  12. Combining modules for movement.

    PubMed

    Bizzi, E; Cheung, V C K; d'Avella, A; Saltiel, P; Tresch, M

    2008-01-01

    We review experiments supporting the hypothesis that the vertebrate motor system produces movements by combining a small number of units of motor output. Using a variety of approaches such as microstimulation of the spinal cord, NMDA iontophoresis, and an examination of natural behaviors in intact and deafferented animals we have provided evidence for a modular organization of the spinal cord. A module is a functional unit in the spinal cord that generates a specific motor output by imposing a specific pattern of muscle activation. Such an organization might help to simplify the production of movements by reducing the degrees of freedom that need to be specified.

  13. 47 CFR 87.141 - Modulation requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... frequency modulated transmitter operating in the band 72.0-76.0 MHz must have a modulation limiter. (f) Each frequency modulated transmitter equipped with a modulation limiter must have a low pass filter between the modulation limiter and the modulated stage. At audio frequencies between 3 kHz and 15 kHz, the filter...

  14. 47 CFR 87.141 - Modulation requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... frequency modulated transmitter operating in the band 72.0-76.0 MHz must have a modulation limiter. (f) Each frequency modulated transmitter equipped with a modulation limiter must have a low pass filter between the modulation limiter and the modulated stage. At audio frequencies between 3 kHz and 15 kHz, the filter...

  15. 47 CFR 87.141 - Modulation requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... frequency modulated transmitter operating in the band 72.0-76.0 MHz must have a modulation limiter. (f) Each frequency modulated transmitter equipped with a modulation limiter must have a low pass filter between the modulation limiter and the modulated stage. At audio frequencies between 3 kHz and 15 kHz, the filter...

  16. Processing module operating methods, processing modules, and communications systems

    DOEpatents

    McCown, Steven Harvey; Derr, Kurt W.; Moore, Troy

    2014-09-09

    A processing module operating method includes using a processing module physically connected to a wireless communications device, requesting that the wireless communications device retrieve encrypted code from a web site and receiving the encrypted code from the wireless communications device. The wireless communications device is unable to decrypt the encrypted code. The method further includes using the processing module, decrypting the encrypted code, executing the decrypted code, and preventing the wireless communications device from accessing the decrypted code. Another processing module operating method includes using a processing module physically connected to a host device, executing an application within the processing module, allowing the application to exchange user interaction data communicated using a user interface of the host device with the host device, and allowing the application to use the host device as a communications device for exchanging information with a remote device distinct from the host device.

  17. Phase modulation in RF tag

    DOEpatents

    Carrender, Curtis Lee; Gilbert, Ronald W.

    2007-02-20

    A radio frequency (RF) communication system employs phase-modulated backscatter signals for RF communication from an RF tag to an interrogator. The interrogator transmits a continuous wave interrogation signal to the RF tag, which based on an information code stored in a memory, phase-modulates the interrogation signal to produce a backscatter response signal that is transmitted back to the interrogator. A phase modulator structure in the RF tag may include a switch coupled between an antenna and a quarter-wavelength stub; and a driver coupled between the memory and a control terminal of the switch. The driver is structured to produce a modulating signal corresponding to the information code, the modulating signal alternately opening and closing the switch to respectively decrease and increase the transmission path taken by the interrogation signal and thereby modulate the phase of the response signal. Alternatively, the phase modulator may include a diode coupled between the antenna and driver. The modulating signal from the driver modulates the capacitance of the diode, which modulates the phase of the response signal reflected by the diode and antenna.

  18. View of Apollo 13 Lunar Module from the Command Module

    NASA Technical Reports Server (NTRS)

    1970-01-01

    This view of the Apollo 13 Lunar Module (LM) was photographed from the Command Module (CM) just after the LM had been jettisoned. The jettisoning occurred a few minutes after 11 a.m., April 17, 1970, just over an hour prior to splashdown of the CM in the South Pacific Ocean. The apparent explosion of oxygen tank number two in the Apoll 13 Service Module caused the Apollo 13 crewmen to rely on the LM as a 'lifeboat'.

  19. Spacelab-1 Module

    NASA Technical Reports Server (NTRS)

    1983-01-01

    This photograph shows the Spacelab-1 module and Spacelab access turnel being installed in the cargo bay of orbiter Columbia for the STS-9 mission. The oribiting laboratory, built by the European Space Agency, is capable of supporting many types of scientific research that can best be performed in space. The Spacelab access tunnel, the only major piece of Spacelab hardware made in the U.S., connects the module with the mid-deck level of the orbiter cabin. The first Spacelab mission, Spacelab-1, sponsored jointly and shared equally by NASA and the European Space Agency, was a multidisciplinary mission; that is, investigations were performed in several different fields of scientific research. The overall goal of the mission was to verify Spacelab performance through a variety of scientific experiments. The disciplines represented by these experiments were: astronomy and solar physics, earth observations, space plasma physics, materials sciences, atmospheric physics, and life sciences. International in nature, Spacelab-1 conducted experiments from the United States, Japan, the Netherlands, United Kingdom, Beluga, France, Germany, Italy, and Switzerland. Spacelab-1, was launched from the Kennedy Space Center on November 28, 1983 aboard the orbiter Columbia (STS-9). The Marshall Space Flight Center was responsible for managing the Spacelab missions.

  20. Externally modulated theranostic nanoparticles

    PubMed Central

    Urban, Cordula; Urban, Alexander S.; Charron, Heather; Joshi, Amit

    2013-01-01

    Externally modulated nanoparticles comprise a rapidly advancing class of cancer nanotherapeutics, which combine the favorable tumor accumulation of nanoparticles, with external spatio-temporal control on therapy delivery via optical, magnetic, or ultrasound modalities. The local control on therapy enables higher tumor treatment efficacy, while simultaneously reducing off-target effects. The nanoparticle interactions with external fields have an additional advantage of frequently generating an imaging signal, and thus such agents provide theranostic (both diagnostic and therapeutic) capabilities. In this review, we classify the emerging externally modulated theranostic nanoparticles according to the mode of external control and describe the physiochemical mechanisms underlying the external control of therapy, and illustrate the major embodiments of nanoparticles in each class with proven biological efficacy: (I) electromagnetic radiation in visible and near-infrared range is being exploited for gold based and carbon nanostructures with tunable surface plasmon resonance (SPR) for imaging and photothermal therapy (PTT) of cancer, photochemistry based manipulations are employed for light sensitive liposomes and porphyrin based nanoparticles; (II) Magnetic field based manipulations are being developed for iron-oxide based nanostructures for magnetic resonance imaging (MRI) and magnetothermal therapy; (III) ultrasound based methods are primarily being employed to increase delivery of conventional drugs and nanotherapeutics to tumor sites. PMID:24834381

  1. Photodynamic immune modulation (PIM)

    NASA Astrophysics Data System (ADS)

    North, John R.; Hunt, David W. C.; Simkin, Guillermo O.; Ratkay, Leslie G.; Chan, Agnes H.; Lui, Harvey; Levy, Julia G.

    1999-09-01

    Photodynamic Therapy (PDT) is accepted for treatment of superficial and lumen-occluding tumors in regions accessible to activating light and is now known to be effective in closure of choroidal neovasculature in Age Related Macular Degeneration. PDT utilizes light absorbing drugs (photosensitizers) that generate the localized formation of reactive oxygen species after light exposure. In a number of systems, PDT has immunomodulatory effects; Photodynamic Immune Modulation (PIM). Using low- intensity photodynamic regimens applied over a large body surface area, progression of mouse autoimmune disease could be inhibited. Further, this treatment strongly inhibited the immunologically- medicated contact hypersensitivity response to topically applied chemical haptens. Immune modulation appears to result from selective targeting of activated T lymphocytes and reduction in immunostimulation by antigen presenting cells. Psoriasis, an immune-mediated skin condition, exhibits heightened epidermal cell proliferation, epidermal layer thickening and plaque formation at different body sites. In a recent clinical trial, approximately one-third of patients with psoriasis and arthritis symptoms (psoriatic arthritis) displayed a significant clinical improvement in several psoriasis-related parameters after four weekly whole-body PIM treatments with verteporfin. The safety profile was favorable. The capacity of PIM to influence other human immune disorders including rheumatoid arthritis is under extensive evaluation.

  2. Externally modulated theranostic nanoparticles.

    PubMed

    Urban, Cordula; Urban, Alexander S; Charron, Heather; Joshi, Amit

    2013-08-01

    Externally modulated nanoparticles comprise a rapidly advancing class of cancer nanotherapeutics, which combine the favorable tumor accumulation of nanoparticles, with external spatio-temporal control on therapy delivery via optical, magnetic, or ultrasound modalities. The local control on therapy enables higher tumor treatment efficacy, while simultaneously reducing off-target effects. The nanoparticle interactions with external fields have an additional advantage of frequently generating an imaging signal, and thus such agents provide theranostic (both diagnostic and therapeutic) capabilities. In this review, we classify the emerging externally modulated theranostic nanoparticles according to the mode of external control and describe the physiochemical mechanisms underlying the external control of therapy, and illustrate the major embodiments of nanoparticles in each class with proven biological efficacy: (I) electromagnetic radiation in visible and near-infrared range is being exploited for gold based and carbon nanostructures with tunable surface plasmon resonance (SPR) for imaging and photothermal therapy (PTT) of cancer, photochemistry based manipulations are employed for light sensitive liposomes and porphyrin based nanoparticles; (II) Magnetic field based manipulations are being developed for iron-oxide based nanostructures for magnetic resonance imaging (MRI) and magnetothermal therapy; (III) ultrasound based methods are primarily being employed to increase delivery of conventional drugs and nanotherapeutics to tumor sites.

  3. Whole Module Offgas Test Report: Space-Xl Dragon Module

    NASA Technical Reports Server (NTRS)

    James, John T.

    2012-01-01

    On September 26 and September 28,2012 a chemist from the JSC Toxicology Group acquired samples of air in 500 m1 evacuated canisters from the sealed Space-Xl Dragon Module. One sample was also acquired from Space-X Facility near the module at the start of the test. Samples of the module air were taken in triplicate once the module had been sealed, and then taken again in triplicate 1.98 days later. Ofthe triplicate samples, the first served as a line purge, and the last two were analyzed. The results of 5 samples are reported.

  4. Modulated preheating and isocurvature perturbations

    SciTech Connect

    Enqvist, Kari; Rusak, Stanislav E-mail: stanislav.rusak@helsinki.fi

    2013-03-01

    We consider a model of preheating where the coupling of the inflaton to the preheat field is modulated by an additional scalar field which is light during inflation. We establish that such a model produces the observed curvature perturbation analogously to the modulated reheating scenario. The contribution of modulated preheating to the power spectrum and to non-Gaussianity can however be significantly larger compared to modulated perturbative reheating. We also consider the implications of the current constraints on isocurvature perturbations in case where the modulating field is responsible for cold dark matter. We find that existing bounds on CDM isocurvature perturbations imply that modulated preheating is unlikely to give a dominant contribution to the curvature perturbation and that the same bounds suggest important constraints on non-Gaussianity and the amount of primordial gravitational waves.

  5. Photovoltaic concentrator module improvements study

    SciTech Connect

    Levy, S.L.; Kerschen, K.A. ); Hutchison, G. ); Nowlan, M.J. )

    1991-08-01

    This report presents results of a project to design and fabricate an improved photovoltaic concentrator module. Using previous work as a baseline, this study conducted analyses and testing to select major module components and design features. The lens parquet and concentrator solar cell were selected from the highest performing, available components. A single 185X point-focus module was fabricated by the project team and tested at Sandia. Major module characteristics include a 6 by 4 compression-molded acrylic lens parquet (0.737 m{sup 2} area), twenty-four 0.2 ohms-cm, FZ, p-Si solar cells (1.56 cm{sup 2} area) soldered to ceramic substrates and copper heat spreaders, and an aluminized steel housing with corrugated bottom. This project marked the first attempt to use prismatic covers on solar cells in a high-concentration, point-focus application. Cells with 15 percent metallization were obtained, but problems with the fabrication and placement of prismatic covers on these cells lead to the decision not to use covers in the prototype module. Cell assembly fabrication, module fabrication, and module optical design activities are presented here. Test results are also presented for bare cells, cell assemblies, and module. At operating conditions of 981 watts/m{sup 2} DNI and an estimated cell temperature of 65{degrees}C, the module demonstrated an efficiency of 13.9 percent prior to stressed environmental exposure. 12 refs., 56 figs., 7 tabs.

  6. International Energy Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Summarizes the overall structure of the International Energy Model and its interface with other NEMS modules, mathematical specifications of behavioral relationships, and data sources and estimation methods.

  7. Water-module interaction studies

    NASA Technical Reports Server (NTRS)

    Mon, G.; Wen, L.; Ross, R., Jr.

    1988-01-01

    Mechanisms by which moisture enters photovoltaic modules and techniques for reducing such interactions are reported. Results from a study of the effectiveness of various module sealants are given. Techniques for measuring the rate and quantity of moisture ingress are discussed. It is shown that scribe lines and porous frit bridging conductors provide preferential paths for moisture ingress and that moisture diffusion by surface/interfacial paths is considerably more rapid than diffusion by bulk paths, which implies that thin-film substrate and supersubstrate modules are much more vulnerable to moist environments than are bulk-encapsulated crystalline-silicon modules. Design approaches that reduce moisture entry are discussed.

  8. Water-module interaction studies

    NASA Astrophysics Data System (ADS)

    Mon, G.; Wen, L.; Ross, R., Jr.

    Mechanisms by which moisture enters photovoltaic modules and techniques for reducing such interactions are reported. Results from a study of the effectiveness of various module sealants are given. Techniques for measuring the rate and quantity of moisture ingress are discussed. It is shown that scribe lines and porous frit bridging conductors provide preferential paths for moisture ingress and that moisture diffusion by surface/interfacial paths is considerably more rapid than diffusion by bulk paths, which implies that thin-film substrate and supersubstrate modules are much more vulnerable to moist environments than are bulk-encapsulated crystalline-silicon modules. Design approaches that reduce moisture entry are discussed.

  9. Adjustable extender for instrument module

    DOEpatents

    Sevec, J.B.; Stein, A.D.

    1975-11-01

    A blank extender module used to mount an instrument module in front of its console for repair or test purposes has been equipped with a rotatable mount and means for locking the mount at various angles of rotation for easy accessibility. The rotatable mount includes a horizontal conduit supported by bearings within the blank module. The conduit is spring-biased in a retracted position within the blank module and in this position a small gear mounted on the conduit periphery is locked by a fixed pawl. The conduit and instrument mount can be pulled into an extended position with the gear clearing the pawl to permit rotation and adjustment of the instrument.

  10. MoNetFamily: a web server to infer homologous modules and module-module interaction networks in vertebrates.

    PubMed

    Lin, Chun-Yu; Lin, Yi-Wei; Yu, Shang-Wen; Lo, Yu-Shu; Yang, Jinn-Moon

    2012-07-01

    A module is a fundamental unit forming with highly connected proteins and performs a certain kind of biological functions. Modules and module-module interaction (MMI) network are essential for understanding cellular processes and functions. The MoNetFamily web server can identify the modules, homologous modules (called module family) and MMI networks across multiple species for the query protein(s). This server first finds module candidates of the query by using BLASTP to search the module template database (1785 experimental and 1252 structural templates). MoNetFamily then infers the homologous modules of the selected module candidate using protein-protein interaction (PPI) families. According to homologous modules and PPIs, we statistically calculated MMIs and MMI networks across multiple species. For each module candidate, MoNetFamily identifies its neighboring modules and their MMIs in module networks of Homo sapiens, Mus musculus and Danio rerio. Finally, MoNetFamily shows the conserved proteins, PPI profiles and functional annotations of the module family. Our results indicate that the server can be useful for MMI network (e.g. 1818 modules and 9678 MMIs in H. sapiens) visualizations and query annotations using module families and neighboring modules. We believe that the server is able to provide valuable insights to determine homologous modules and MMI networks across multiple species for studying module evolution and cellular processes. The MoNetFamily sever is available at http://monetfamily.life.nctu.edu.tw.

  11. Light modulating device

    SciTech Connect

    Rauh, R.D.; Goldner, R.B.

    1989-12-26

    In a device for transmitting light, means for controlling the transmissivity of the device, including a ceramic, reversibly electrochromic, crystalline element having a highly reflective state when injected with electrons and charge compensating ions and a highly transmissive state when the electrons and ions are removed, the crystalline element being characterized as having a reflectivity of at least 50% in the reflective state and not greater than 10% in the transmissive state, and means for modulating the crystalline element between the reflective and transmissive states by injecting ions into the crystalline element in response to an applied electrical current of a first polarity and removing the ions in response to an applied electrical current of a second polarity are disclosed. 1 fig.

  12. Automatic routing module

    NASA Technical Reports Server (NTRS)

    Malin, Janice A.

    1987-01-01

    Automatic Routing Module (ARM) is a tool to partially automate Air Launched Cruise Missile (ALCM) routing. For any accessible launch point or target pair, ARM creates flyable routes that, within the fidelity of the models, are optimal in terms of threat avoidance, clobber avoidance, and adherence to vehicle and planning constraints. Although highly algorithmic, ARM is an expert system. Because of the heuristics applied, ARM generated routes closely resemble manually generated routes in routine cases. In more complex cases, ARM's ability to accumulate and assess threat danger in three dimensions and trade that danger off with the probability of ground clobber results in the safest path around or through difficult areas. The tools available prior to ARM did not provide the planner with enough information or present it in such a way that ensured he would select the safest path.

  13. Fortran 90 Source Module

    1998-01-13

    A major advance contained in the new Fortran 90 language standard is the ability to define new data types and the operators associated with them. Writing computer code to implement computations with real and complex three domensional vectors and dyadics is greatly simplified if the equations can be implemented directly, without the need to code the vector arithmetic explicitly. The Fortran 90 module VECTORS contains source code which defines new data types for real andmore » complex 3-dimensional vectors and dyadics, along with the common operations needed to work with these objects. Routines to allow convenient initalization and output of the new types are also included. in keeping with the philosophy of data abstraction, the details of the implementation of the data types are maintained private, and the functions and operators are made generic to simplify the combining of real, complex, single and double precision vectors and dyadics.« less

  14. Photovoltaic module mounting system

    SciTech Connect

    Miros, Robert H. J.; Mittan, Margaret Birmingham; Seery, Martin N; Holland, Rodney H

    2012-09-18

    A solar array mounting system having unique installation, load distribution, and grounding features, and which is adaptable for mounting solar panels having no external frame. The solar array mounting system includes flexible, pedestal-style feet and structural links connected in a grid formation on the mounting surface. The photovoltaic modules are secured in place via the use of attachment clamps that grip the edge of the typically glass substrate. The panel mounting clamps are then held in place by tilt brackets and/or mid-link brackets that provide fixation for the clamps and align the solar panels at a tilt to the horizontal mounting surface. The tilt brackets are held in place atop the flexible feet and connected link members thus creating a complete mounting structure.

  15. Photovoltaic module mounting system

    SciTech Connect

    Miros, Robert H. J.; Mittan, Margaret Birmingham; Seery, Martin N.; Holland, Rodney H.

    2012-04-17

    A solar array mounting system having unique installation, load distribution, and grounding features, and which is adaptable for mounting solar panels having no external frame. The solar array mounting system includes flexible, pedestal-style feet and structural links connected in a grid formation on the mounting surface. The photovoltaic modules are secured in place via the use of attachment clamps that grip the edge of the typically glass substrate. The panel mounting clamps are then held in place by tilt brackets and/or mid-link brackets that provide fixation for the clamps and align the solar panels at a tilt to the horizontal mounting surface. The tilt brackets are held in place atop the flexible feet and connected link members thus creating a complete mounting structure.

  16. Light modulating device

    DOEpatents

    Rauh, R. David; Goldner, Ronald B.

    1989-01-01

    In a device for transmitting light, means for controlling the transmissivity of the device, including a ceramic, reversibly electrochromic, crystalline element having a highly reflective state when injected with electrons and charge compensating ions and a highly transmissive state when the electrons and ions are removed, the crystalline element being characterized as having a reflectivity of at least 50% in the reflective state and not greater than 10% in the transmissive state, and means for modulating the crystalline element between the reflective and transmissive states by injecting ions into the crystalline element in response to an applied electrical current of a first polarity and removing the ions in response to an applied electrical current of a second polarity.

  17. 12. VIEW OF THE INSPECTION MODULE (MODULE D). THE GLOVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW OF THE INSPECTION MODULE (MODULE D). THE GLOVE BOX IN THE FOREFRONT OF THE PHOTOGRAPH CONTAINS A DRILL PRESS; OTHER GLOVE BOXES ARE USED FOR PARTS INSPECTION. (5/70) - Rocky Flats Plant, Plutonium Manufacturing Facility, North-central section of Plant, just south of Building 776/777, Golden, Jefferson County, CO

  18. Low-voltage differentially-signaled modulators

    SciTech Connect

    Zortman, William A.; Lentine, Anthony L.; Hsia, Alexander H.; Watts, Michael R.

    2015-09-08

    Photonic modulators and methods of modulating an input optical signal are provided. A photonic modulator includes at least one modulator section and differential drive circuitry. The at least one modulator section includes a P-type layer and an N-type layer forming a PN junction in the modulator section. The differential drive circuitry is electrically coupled to the P-type layer and the N-type layer of the at least one modulator section.

  19. Thermal modulation for gas chromatography

    NASA Technical Reports Server (NTRS)

    Hasselbrink, Ernest F. (Inventor); Libardoni, Mark (Inventor); Stewart, Kristine (Inventor); Waite, J. Hunter (Inventor); Block, Bruce P. (Inventor); Sacks, Richard D. (Inventor)

    2007-01-01

    A thermal modulator device for gas chromatography and associated methods. The thermal modulator device includes a cooling member, an electrically conductive capillary in direct thermal contact with the cooling member, and a power supply electrically coupled to the capillary and operable for controlled resistive heating of the capillary.

  20. Concentration-modulated absorption spectroscopy.

    PubMed

    Langley, A J; Beaman, R A; Baran, J; Davies, A N; Jones, W J

    1985-07-01

    Concentration modulation is demonstrated to be a technique capable of markedly extending sensitivity limits in absorption spectroscopy. The gain generated relates in such a manner to sample transmittance that for the first reported time direct spectroscopic concentration measurements become possible. When concentration modulation is used with picosecond lasers, state lifetimes can be determined to a limit of approximately 20 psec.

  1. Headstart German Program. Module 10.

    ERIC Educational Resources Information Center

    Defense Language Inst., Monterey, CA.

    This module provides additional material principally for women in the military and female dependents. The three dialogs in the module take place at a cleaner's, at a drugstore, and at the hairdresser's. The vocabulary and dialogs are intended to familiarize the student with words and phrases which might be needed in a situation when the post…

  2. Argonne's SpEC Module

    SciTech Connect

    Harper, Jason

    2014-05-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  3. Aligned natural inflation with modulations

    NASA Astrophysics Data System (ADS)

    Choi, Kiwoon; Kim, Hyungjin

    2016-08-01

    The weak gravity conjecture applied for the aligned natural inflation indicates that generically there can be a modulation of the inflaton potential, with a period determined by sub-Planckian axion scale. We study the oscillations in the primordial power spectrum induced by such modulation, and discuss the resulting observational constraints on the model.

  4. Argonne's SpEC Module

    ScienceCinema

    Harper, Jason

    2016-07-12

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  5. Diagnosis And Prescription: Reinforcement Module.

    ERIC Educational Resources Information Center

    Fair, George W.

    This learning module has been designed to aid the teacher trainee in identifying ways in which he influences student behavior in the classroom and also explores means of selecting more meaningful reinforcers and their application. Terminal objectives of the module are the ability to (1) define the terms "reinforcement,""positive…

  6. Housing and Home Furnishings Modules.

    ERIC Educational Resources Information Center

    Clemson Univ., SC. Vocational Education Media Center.

    These sixty-seven modules provide student materials for a home economics course in housing and home furnishings. (A companion instructor's guide is available separately--see note.) Each module contains an objective, student information, learning activities (and activity sheets as needed), student self-checks, student self-check answers, check-out…

  7. Curriculum Modules in Minority Aging.

    ERIC Educational Resources Information Center

    Wood, Joan B.

    These two curriculum modules are self-contained units focusing on older women of color (primarily African-American) and rural minority elders. The modules were developed as a product of a Model Gerontology Career Development Program in Institutions of Higher Education in Rural Areas through a consortium of colleges, universities, and agencies…

  8. Receivers for modulated digital signals

    NASA Astrophysics Data System (ADS)

    Troendle, K.; Huber, J.

    The realization principles for coherent and incoherent receivers for modulated digital signal transmission are presented. From these principles, the digital amplitude, phase, and frequency modulation are treated. Expressions for the error probabilities in these systems are given, and their required bandwidths and noise sensitivities are compared. Principles of system realization for time-variant channels and methods of clock and carrier recovery are explained.

  9. Macroeconomic Activity Module - NEMS Documentation

    EIA Publications

    2016-01-01

    Documents the objectives, analytical approach, and development of the National Energy Modeling System (NEMS) Macroeconomic Activity Module (MAM) used to develop the Annual Energy Outlook for 2016 (AEO2016). The report catalogues and describes the module assumptions, computations, methodology, parameter estimation techniques, and mainframe source code

  10. Input/output interface module

    NASA Technical Reports Server (NTRS)

    Ozyazici, E. M.

    1980-01-01

    Module detects level changes in any of its 16 inputs, transfers changes to its outputs, and generates interrupts when changes are detected. Up to four changes-in-state per line are stored for later retrieval by controlling computer. Using standard TTL logic, module fits 19-inch rack-mounted console.

  11. Telescoping Space-Station Modules

    NASA Technical Reports Server (NTRS)

    Witcofski, R. D.

    1986-01-01

    New telescoping-space-station design involves module within a module. After being carried to orbit within payload bay of Space Shuttle orbiter, outer module telescopically deployed to achieve nearly twice as much usable space-station volume per Space Shuttle launch. Closed-loop or "race-track" space-station configurations possible with this concept and provide additional benefits. One benefit involves making one of modules double-walled haven safe from debris, radiation, and like. Module accessible from either end, and readily available to all positions in space station. Concept also provides flexibility in methods in which Space Shuttle orbiter docked or berthed with space station and decrease chances of damage.

  12. Acceleration Recorder and Playback Module

    NASA Technical Reports Server (NTRS)

    Bozeman, Richard J., Jr. (Inventor)

    1996-01-01

    The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

  13. Acceleration recorder and playback module

    NASA Technical Reports Server (NTRS)

    Bozeman, Richard J., Jr. (Inventor)

    1994-01-01

    The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

  14. Phase-Controlled Polarization Modulators

    NASA Technical Reports Server (NTRS)

    Chuss, D. T.; Wollack, E. J.; Novak, G.; Moseley, S. H.; Pisano, G.; Krejny, M.; U-Yen, K.

    2012-01-01

    We report technology development of millimeter/submillimeter polarization modulators that operate by introducing a a variable, controlled phase delay between two orthogonal polarization states. The variable-delay polarization modulator (VPM) operates via the introduction of a variable phase delay between two linear orthogonal polarization states, resulting in a variable mapping of a single linear polarization into a combination of that Stokes parameter and circular (Stokes V) polarization. Characterization of a prototype VPM is presented at 350 and 3000 microns. We also describe a modulator in which a variable phase delay is introduced between right- and left- circular polarization states. In this architecture, linear polarization is fully modulated. Each of these devices consists of a polarization diplexer parallel to and in front of a movable mirror. Modulation involves sub-wavelength translations of the mirror that change the magnitude of the phase delay.

  15. Acceleration recorder and playback module

    NASA Astrophysics Data System (ADS)

    Bozeman, Richard J., Jr.

    1994-11-01

    The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

  16. Acceleration recorder and playback module

    NASA Astrophysics Data System (ADS)

    Bozeman, Richard J., Jr.

    1992-09-01

    The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

  17. Force Modulator System

    SciTech Connect

    Redmond Clark

    2009-04-30

    Many metal parts manufacturers use large metal presses to shape sheet metal into finished products like car body parts, jet wing and fuselage surfaces, etc. These metal presses take sheet metal and - with enormous force - reshape the metal into a fully formed part in a manner of seconds. Although highly efficient, the forces involved in forming metal parts also damage the press itself, limit the metals used in part production, slow press operations and, when not properly controlled, cause the manufacture of large volumes of defective metal parts. To date, the metal-forming industry has not been able to develop a metal-holding technology that allows full control of press forces during the part forming process. This is of particular importance in the automotive lightweighting efforts under way in the US automotive manufacturing marketplace. Metalforming Controls Technology Inc. (MC2) has developed a patented press control system called the Force Modulator that has the ability to control these press forces, allowing a breakthrough in stamping process control. The technology includes a series of hydraulic cylinders that provide controlled tonnage at all points in the forming process. At the same time, the unique cylinder design allows for the generation of very high levels of clamping forces (very high tonnages) in very small spaces; a requirement for forming medium and large panels out of HSS and AHSS. Successful production application of these systems testing at multiple stamping operations - including Ford and Chrysler - has validated the capabilities and economic benefits of the system. Although this technology has been adopted in a number of stamping operations, one of the primary barriers to faster adoption and application of this technology in HSS projects is system cost. The cost issue has surfaced because the systems currently in use are built for each individual die as a custom application, thus driving higher tooling costs. This project proposed to better

  18. Modulator-free quadrature amplitude modulation signal synthesis

    PubMed Central

    Liu, Zhixin; Kakande, Joseph; Kelly, Brian; O’Carroll, John; Phelan, Richard; Richardson, David J.; Slavík, Radan

    2014-01-01

    The ability to generate high-speed on–off-keyed telecommunication signals by directly modulating a semiconductor laser’s drive current was one of the most exciting prospective applications of the nascent field of laser technology throughout the 1960s. Three decades of progress led to the commercialization of 2.5 Gbit s−1-per-channel submarine fibre optic systems that drove the growth of the internet as a global phenomenon. However, the detrimental frequency chirp associated with direct modulation forced industry to use external electro-optic modulators to deliver the next generation of on–off-keyed 10 Gbit s−1 systems and is absolutely prohibitive for today’s (>)100 Gbit s−1 coherent systems, which use complex modulation formats (for example, quadrature amplitude modulation). Here we use optical injection locking of directly modulated semiconductor lasers to generate complex modulation format signals showing distinct advantages over current and other currently researched solutions. PMID:25523757

  19. Modulator-free quadrature amplitude modulation signal synthesis

    NASA Astrophysics Data System (ADS)

    Liu, Zhixin; Kakande, Joseph; Kelly, Brian; O'Carroll, John; Phelan, Richard; Richardson, David J.; Slavík, Radan

    2014-12-01

    The ability to generate high-speed on-off-keyed telecommunication signals by directly modulating a semiconductor laser’s drive current was one of the most exciting prospective applications of the nascent field of laser technology throughout the 1960s. Three decades of progress led to the commercialization of 2.5 Gbit s-1-per-channel submarine fibre optic systems that drove the growth of the internet as a global phenomenon. However, the detrimental frequency chirp associated with direct modulation forced industry to use external electro-optic modulators to deliver the next generation of on-off-keyed 10 Gbit s-1 systems and is absolutely prohibitive for today’s (>)100 Gbit s-1 coherent systems, which use complex modulation formats (for example, quadrature amplitude modulation). Here we use optical injection locking of directly modulated semiconductor lasers to generate complex modulation format signals showing distinct advantages over current and other currently researched solutions.

  20. Modulated Source Interferometry with Combined Amplitude and Frequency Modulation

    NASA Technical Reports Server (NTRS)

    Gutierrez, Roman C. (Inventor)

    1998-01-01

    An improved interferometer is produced by modifying a conventional interferometer to include amplitude and/or frequency modulation of a coherent light source at radio or higher frequencies. The phase of the modulation signal can be detected in an interfering beam from an interferometer and can be used to determine the actual optical phase of the beam. As such, this improvement can be adapted to virtually any two-beam interferometer, including: Michelson, Mach-Zehnder, and Sagnac interferometers. The use of an amplitude modulated coherent tight source results in an interferometer that combines the wide range advantages of coherent interferometry with the precise distance measurement advantages of white light interferometry.

  1. Programmable Thermostat Module Upgrade for the Multipurpose Logistics Module

    NASA Technical Reports Server (NTRS)

    Clark, D. W.; Glasgow, S. d.; Reagan, S. E.; Presson, K. H.; Howard, D. E.; Smith, D. A.

    2007-01-01

    The STS-121/ULF 1.1 mission was the maiden flight of the programmable thermostat module (PTM) system used to control the 28 V shell heaters on the multi-purpose logistics module (MPLM). These PTMs, in conjunction with a data recorder module (DRM), provide continuous closed loop temperature control and data recording of MPLM on-orbit heater operations. This Technical Memorandum discusses the hardware design, development, test, and verification (DDT&V) activities performed at the Marshall Space Flight Center as well as the operational implementation and mission performance.

  2. Photovoltaic solar concentrator module

    SciTech Connect

    Chiang, C.J.

    1991-05-16

    This invention consists of a planar photovoltaic concentrator module for producing an electrical signal from incident solar radiation which includes an electrically insulating housing having a front wall, an opposing back wall and a hollow interior. A solar cell having electrical terminals is positioned within the interior of the housing. A planar conductor is connected with a terminal of the solar cell of the same polarity. A lens forming the front wall of the housing is operable to direct solar radiation incident to the lens into the interior of the housing. A refractive optical element in contact with the solar cell and facing the lens receives the solar radiation directed into the interior of the housing by the lens and directs the solar radiation to the solar cell to cause the solar cell to generate an electrical signal. An electrically conductive planar member is positioned in the housing to rest on the housing back wall in supporting relation with the solar cell terminal of opposite polarity. The planar member is operable to dissipate heat radiated by the solar cell as the solar cell generates an electrical signal and further forms a solar cell conductor connected with the solar cell terminal to permit the electrical signal generated by the solar cell to be measured between the planar member and the conductor.

  3. Selective Estrogen Receptor Modulators.

    PubMed

    An, Ki-Chan

    2016-08-01

    Selective estrogen receptor modulators (SERMs) are now being used as a treatment for breast cancer, osteoporosis and postmenopausal symptoms, as these drugs have features that can act as an estrogen agonist and an antagonist, depending on the target tissue. After tamoxifen, raloxifene, lasofoxifene and bazedoxifene SERMs have been developed and used for treatment. The clinically decisive difference among these drugs (i.e., the key difference) is their endometrial safety. Compared to bisphosphonate drug formulations for osteoporosis, SERMs are to be used primarily in postmenopausal women of younger age and are particularly recommended if there is a family history of invasive breast cancer, as their use greatly reduces the incidence of this type of cancer in women. Among the above mentioned SERMs, raloxifene has been widely used in prevention and treatment of postmenopausal osteoporosis and vertebral compression fractures, and clinical studies are now underway to test the comparative advantages of raloxifene with those of bazedoxifene, a more recently developed SERM. Research on a number of adverse side effects of SERM agents is being performed to determine the long-term safety of this class of compouds for treatment of osteoporosis. PMID:27559463

  4. OCGen Module Mooring Project

    SciTech Connect

    McEntee, Jarlath

    2015-02-06

    Ocean Renewable Power Company's OCGen Module Mooring Project provided an extensive research, design, development, testing and data collection effort and analysis conducted with respect to a positively buoyant, submerged MHK device secured to the seabed using a tensioned mooring system. Different analytic tools were evaluated for their utility in the design of submerged systems and their moorings. Deployment and testing of a prototype OCGen® system provided significant data related to mooring line loads and system attitude and station keeping. Mooring line loads were measured in situ and reported against flow speeds. The Project made a significant step in the development of designs, methodologies and practices related to floating and mooring of marine hydrokinetic (MHK) devices. Importantly for Ocean Renewable Power Company, the Project provided a sound basis for advancing a technically and commercially viable OCGen® Power System. The OCGen® Power System is unique in the MHK industry and, in itself, offers distinct advantages of MHK devices that are secured to the seabed using fixed structural frames. Foremost among these advantages are capital and operating cost reductions and increased power extraction by allowing the device to be placed at the most energetic level of the water column.

  5. Plug cluster module demonstration

    NASA Technical Reports Server (NTRS)

    Rousar, D. C.

    1978-01-01

    The low pressure, film cooled rocket engine design concept developed during two previous ALRC programs was re-evaluated for application as a module for a plug cluster engine capable of performing space shuttle OTV missions. The nominal engine mixture ratio was 5.5 and the engine life requirements were 1200 thermal cycles and 10 hours total operating life. The program consisted of pretest analysis; engine tests, performed using residual components; and posttest analysis. The pretest analysis indicated that operation of the operation of the film cooled engine at O/F = 5.5 was feasible. During the engine tests, steady state wall temperature and performance measurement were obtained over a range of film cooling flow rates, and the durability of the engine was demonstrated by firing the test engine 1220 times at a nominal performance ranging from 430 - 432 seconds. The performance of the test engine was limited by film coolant sleeve damage which had occurred during previous testing. The post-test analyses indicated that the nominal performance level can be increased to 436 seconds.

  6. Noradrenergic Modulation of Arousal

    PubMed Central

    Berridge, Craig W.

    2008-01-01

    Through a highly divergent efferent projection system, the locus coeruleus-noradrenergic system supplies norepinephrine throughout the central nervous system. State-dependent neuronal discharge activity of locus coeruleus neurons has long-suggested a role of this system in the induction of an alert waking state. More recent work supports this hypothesis, demonstrating robust wake-promoting actions of the locus coeruleus-noradrenergic system. Norepinephrine enhances arousal, in part, via actions of β- and α1-receptors located within multiple subcortical structures, including the general regions of the medial septal area and the medial preoptic areas. Recent anatomical studies suggest that arousal-enhancing actions of norepinephrine are not limited to the locus coeruleus system and likely include the A1 and A2 noradrenergic cell groups. Thus, noradrenergic modulation of arousal state involves multiple noradrenergic systems acting with multiple subcortical regions. Pharmacological studies indicate that the combined actions of these systems are necessary for the sustained maintenance of arousal levels associated with spontaneous waking. Enhanced arousal state is a prominent aspect of both stress and psychostimulant drug action and evidence indicates that noradrenergic systems likely play an important role in both stress-related and psychostimulant-induced arousal. These and other observations suggest that the dysregulation of noradrenergic neurotransmission could well contribute to the dysregulation of arousal associated with a variety of behavioral disorders including insomnia and stress-related disorders. PMID:18199483

  7. Living Systems Energy Module

    SciTech Connect

    1995-09-26

    The Living Systems Energy Module, renamed Voyage from the Sun, is a twenty-lesson curriculum designed to introduce students to the major ways in which energy is important in living systems. Voyage from the Sun tells the story of energy, describing its solar origins, how it is incorporated into living terrestrial systems through photosynthesis, how it flows from plants to herbivorous animals, and from herbivores to carnivores. A significant part of the unit is devoted to examining how humans use energy, and how human impact on natural habitats affects ecosystems. As students proceed through the unit, they read chapters of Voyage from the Sun, a comic book that describes the flow of energy in story form (Appendix A). During the course of the unit, an ``Energy Pyramid`` is erected in the classroom. This three-dimensional structure serves as a classroom exhibit, reminding students daily of the importance of energy and of the fragile nature of our living planet. Interactive activities teach students about adaptations that allow plants and animals to acquire, to use and to conserve energy. A complete list of curricular materials and copies of all activity sheets appear in Appendix B.

  8. Common Geometry Module

    2005-01-01

    The Common Geometry Module (CGM) is a code library which provides geometry functionality used for mesh generation and other applications. This functionality includes that commonly found in solid modeling engines, like geometry creation, query and modification; CGM also includes capabilities not commonly found in solid modeling engines, like geometry decomposition tools and support for shared material interfaces. CGM is built upon the ACIS solid modeling engine, but also includes geometry capability developed beside and onmore » top of ACIS. CGM can be used as-is to provide geometry functionality for codes needing this capability. However, CGM can also be extended using derived classes in C++, allowing the geometric model to serve as the basis for other applications, for example mesh generation. CGM is supported on Sun Solaris, SGI, HP, IBM, DEC, Linux and Windows NT platforms. CGM also indudes support for loading ACIS models on parallel computers, using MPI-based communication. Future plans for CGM are to port it to different solid modeling engines, including Pro/Engineer or SolidWorks. CGM is being released into the public domain under an LGPL license; the ACIS-based engine is available to ACIS licensees on request.« less

  9. Selective Estrogen Receptor Modulators

    PubMed Central

    2016-01-01

    Selective estrogen receptor modulators (SERMs) are now being used as a treatment for breast cancer, osteoporosis and postmenopausal symptoms, as these drugs have features that can act as an estrogen agonist and an antagonist, depending on the target tissue. After tamoxifen, raloxifene, lasofoxifene and bazedoxifene SERMs have been developed and used for treatment. The clinically decisive difference among these drugs (i.e., the key difference) is their endometrial safety. Compared to bisphosphonate drug formulations for osteoporosis, SERMs are to be used primarily in postmenopausal women of younger age and are particularly recommended if there is a family history of invasive breast cancer, as their use greatly reduces the incidence of this type of cancer in women. Among the above mentioned SERMs, raloxifene has been widely used in prevention and treatment of postmenopausal osteoporosis and vertebral compression fractures, and clinical studies are now underway to test the comparative advantages of raloxifene with those of bazedoxifene, a more recently developed SERM. Research on a number of adverse side effects of SERM agents is being performed to determine the long-term safety of this class of compouds for treatment of osteoporosis. PMID:27559463

  10. Membrane mirror light modulator technology

    NASA Astrophysics Data System (ADS)

    Warde, Cardinal; McCann, James T.; Shrauger, Vern; Ieong, H.-H.; Ersen, Ali; Wang, X. Y.; Hubbard, J.

    2000-03-01

    We have incorporated membrane mirror technology over a discrete array of pixel wells to create both high-efficiency optical shutters and spatial light modulators (SLM). A continuous metalized-membrane mirror with greater than 98% reflectivity minimizes optical insertion loss. This mirror is electrostatically deformed into the wells with either a common electrode (shutter) or pixilated electrodes (SLM). By using a spatial filter, analog intensity optical modulation is realized. Both 1-D (linear) and 2-D grating pixel patterns have been investigated. With the appropriate pixel dimensions, both coherent monochromatic and broadband incoherent light within the 0.25 to 10.6 micron range can be modulated with contrast ratios up to 1000:1. Small well sizes (approximately 10-micron diameter) allow for modulation speeds up to 1 MHz. The theoretical foundations for the well layout, the membrane mirror deformation and its diffraction properties, and the design trade-offs are detailed. We have applied our membrane mirror technology to CMOS VLSI circuits creating a high-speed, high-efficiency spatial light modulator capable of 80 X 64 resolution and scalable to HDTV standards. The membrane mirror SLM provides either amplitude or phase modulation. In the phase modulation mode, at least two waves of stroke per discrete well are possible.

  11. Intermediate load modules for test and evaluation

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Photovoltaic modules were tested for qualification. Tests involved the following: (1) delivery of 20 solar cells for use as reference cells; (2) module documentation and inspection plans specifying the 10 Group I modules; (3) design review of module documentation from Group I modules; (4) revise module documentation to overcome any problems of deficiencies associated with the Group I modules; (5) delivery of 10 Group II modules built to revised specifications; (6) testing of Group II modules to the criteria as outlined in qualification specification. It is found that the solarvolt MSP43E40B satisfies the design criteria of qualification specification for intermediate load modules. Design changes were made in the Group I modules to overcome the deficiencies which allowed Group II modules to pass the qualification tests.

  12. VIBRATING PERVAPORATION MODULES: EFFECT OF MODULE DESIGN ON PERFORMANCE

    EPA Science Inventory

    A third commercial-scale vibrating pervaporation membrane module was fabricated and evaluated for the separation of volatile organic compounds (VOCs) from aqueous solutions. Experiments with surrogate solutions of four hydrophobic VOCs (1,1,1-trichloroethane (TCA), trichloroethy...

  13. Variable Coded Modulation software simulation

    NASA Astrophysics Data System (ADS)

    Sielicki, Thomas A.; Hamkins, Jon; Thorsen, Denise

    This paper reports on the design and performance of a new Variable Coded Modulation (VCM) system. This VCM system comprises eight of NASA's recommended codes from the Consultative Committee for Space Data Systems (CCSDS) standards, including four turbo and four AR4JA/C2 low-density parity-check codes, together with six modulations types (BPSK, QPSK, 8-PSK, 16-APSK, 32-APSK, 64-APSK). The signaling protocol for the transmission mode is based on a CCSDS recommendation. The coded modulation may be dynamically chosen, block to block, to optimize throughput.

  14. High-Efficiency Power Module

    NASA Technical Reports Server (NTRS)

    Simons, Rainee N. (Inventor); Wintucky, Edwin G. (Inventor)

    2015-01-01

    One or more embodiments of the present invention pertain to an all solid-state microwave power module. The module includes a plurality of solid-state amplifiers configured to amplify a signal using a low power stage, a medium power stage, and a high power stage. The module also includes a power conditioner configured to activate a voltage sequencer (e.g., bias controller) when power is received from a power source. The voltage sequencer is configured to sequentially apply voltage to a gate of each amplifier and sequentially apply voltage to a drain of each amplifier.

  15. Light intensity modulation in phototherapy

    NASA Astrophysics Data System (ADS)

    Lukyanovich, P. A.; Zon, B. A.; Kunin, A. A.; Pankova, S. N.

    2015-04-01

    A hypothesis that blocking ATP synthesis is one of the main causes of the stimulating effect is considered based on analysis of the primary photostimulation mechanisms. The light radiation intensity modulation is substantiated and the estimates of such modulation parameters are made. An explanation is offered to the stimulation efficiency decrease phenomenon at the increase of the radiation dose during the therapy. The results of clinical research of the medical treatment in preventive dentistry are presented depending on the spectrum and parameters of the light flux modulation.

  16. High-Efficiency Power Module

    NASA Technical Reports Server (NTRS)

    Simons, Rainee N (Inventor); Wintucky, Edwin G (Inventor)

    2013-01-01

    One or more embodiments of the present invention pertain to an all solid-state microwave power module. The module includes a plurality of solid-state amplifiers configured to amplify a signal using a low power stage, a medium power stage, and a high power stage. The module also includes a power conditioner configured to activate a voltage sequencer (e.g., bias controller) when power is received from a power source. The voltage sequencer is configured to sequentially apply voltage to a gate of each amplifier and sequentially apply voltage to a drain of each amplifier.

  17. Wound modulation after filtration surgery.

    PubMed

    Seibold, Leonard K; Sherwood, Mark B; Kahook, Malik Y

    2012-11-01

    Filtration surgery is the standard invasive procedure for the management of intraocular pressure in advanced glaucoma. The key to a successful outcome is to modulate the normal wound healing cascade that leads to closure of the newly created aqueous outflow pathway. Antifibrotic agents such as mitomycin C and 5-fluorouracil have been increasingly used to modulate the wound healing process and increase surgical success. Although these agents have proven efficacy, they also increase the risk of complications. Efforts have centered on the identification of novel agents and techniques that can influence wound modulation without these complications. We detail new agents and methods under investigation to control wound healing after filtration surgery. PMID:23068975

  18. High Order Modulation Protograph Codes

    NASA Technical Reports Server (NTRS)

    Nguyen, Thuy V. (Inventor); Nosratinia, Aria (Inventor); Divsalar, Dariush (Inventor)

    2014-01-01

    Digital communication coding methods for designing protograph-based bit-interleaved code modulation that is general and applies to any modulation. The general coding framework can support not only multiple rates but also adaptive modulation. The method is a two stage lifting approach. In the first stage, an original protograph is lifted to a slightly larger intermediate protograph. The intermediate protograph is then lifted via a circulant matrix to the expected codeword length to form a protograph-based low-density parity-check code.

  19. Photovoltaic module with adhesion promoter

    DOEpatents

    Xavier, Grace

    2013-10-08

    Photovoltaic modules with adhesion promoters and methods for fabricating photovoltaic modules with adhesion promoters are described. A photovoltaic module includes a solar cell including a first surface and a second surface, the second surface including a plurality of interspaced back-side contacts. A first glass layer is coupled to the first surface by a first encapsulating layer. A second glass layer is coupled to the second surface by a second encapsulating layer. At least a portion of the second encapsulating layer is bonded directly to the plurality of interspaced back-side contacts by an adhesion promoter.

  20. Photovoltaic Cz Silicon Module Improvements

    SciTech Connect

    Jester, T. L.

    1998-09-01

    Work focused on reducing the cost per watt of Cz silicon photovoltaic modules under Phase II of Siemens Solar Industries' DOE/NREL PVMaT 4A subcontract is described in this report. New module designs were deployed in this phase of the contract, improvements in yield of over 10% were realized, and further implementation of Statistical Process Control was achieved during this phase. Module configurations representing a 12% cost reduction per watt were implemented in small scale production under Phase II of this contract. Yield improvements are described in detail, yield sensitivity to wafer thickness is quantified, and the deployment of SPC in critical process steps is reported here.

  1. Miniature Intelligent Sensor Module

    NASA Technical Reports Server (NTRS)

    Beech, Russell S.

    2007-01-01

    An electronic unit denoted the Miniature Intelligent Sensor Module performs sensor-signal-conditioning functions and local processing of sensor data. The unit includes four channels of analog input/output circuitry, a processor, volatile and nonvolatile memory, and two Ethernet communication ports, all housed in a weathertight enclosure. The unit accepts AC or DC power. The analog inputs provide programmable gain, offset, and filtering as well as shunt calibration and auto-zeroing. Analog outputs include sine, square, and triangular waves having programmable frequencies and amplitudes, as well as programmable amplitude DC. One innovative aspect of the design of this unit is the integration of a relatively powerful processor and large amount of memory along with the sensor-signalconditioning circuitry so that sophisticated computer programs can be used to acquire and analyze sensor data and estimate and track the health of the overall sensor-data-acquisition system of which the unit is a part. The unit includes calibration, zeroing, and signalfeedback circuitry to facilitate health monitoring. The processor is also integrated with programmable logic circuitry in such a manner as to simplify and enhance acquisition of data and generation of analog outputs. A notable unique feature of the unit is a cold-junction compensation circuit in the back shell of a sensor connector. This circuit makes it possible to use Ktype thermocouples without compromising a housing seal. Replicas of this unit may prove useful in industrial and manufacturing settings - especially in such large outdoor facilities as refineries. Two features can be expected to simplify installation: the weathertight housings should make it possible to mount the units near sensors, and the Ethernet communication capability of the units should facilitate establishment of communication connections for the units.

  2. Modulation of lymphopoiesis

    SciTech Connect

    Rosse, C.

    1991-01-01

    During the current project period we have demonstrated correspondence between animal models and in vitro models of modulated lymphopoiesis. Our finding that G-CSF, a growth factor for neutrophil granulocytes, suppresses lymphopoiesis in long term bone marrow cultures (LTBMC) has important implications both for understanding the regulatory mechanisms of hemopoiesis and for clinical use of recombinant growth factors that are beginning to be widely used for the treatment of a variety of diseases. During the present project period we adopted LTBMC systems developed by others for the purposes of our specific aims. Also we developed a novel long term culture system for NK cells. The discovery of a new growth factor, O-CSF, specific for osteoclasts and the establishment of a clonal assay system that provides evidence for a new class of hemopoietic progenitor cells, the osteoclast progenitor, are important contributions. Given the important role T cells play in the immune response and in the regulation of other lymphohemopoietic cell lineages through the lymphokines they secrete, the need for an in vitro system that lends itself to the analysis of T cell maturation and to the testing of factors that may adversely affect T lymphopoiesis cannot be overemphasized. We believe that we can exploit an advantageous set of circumstances that present an excellent opportunity for initiating a focused experimental program for developing such a system. By a systematic and selective analysis of molecular interactions between heterogenous thymic stromal cells and T cell progenitors at different stages of maturation, it will be possible for our program to define the complement of critical cellular interactions on which successive stages of T lymphopoiesis depend. The experiments we propose will lay a rational foundation for the development of a long term culture system for T lymphopoiesis. 24 refs., 7 figs.

  3. Cosmic ray modulation

    NASA Astrophysics Data System (ADS)

    Agarwal Mishra, Rekha; Mishra, Rajesh Kumar

    2016-07-01

    Propagation of cosmic rays to and inside the heliosphere, encounter an outward moving solar wind with cyclic magnetic field fluctuation and turbulence, causing convection and diffusion in the heliosphere. Cosmic ray counts from the ground ground-based neutron monitors at different cut of rigidity show intensity changes, which are anti-correlated with sunspot numbers. They also lose energy as they propagate towards the Earth and experience various types of modulations due to different solar activity indices. In this work, we study the first three harmonics of cosmic ray intensity on geo-magnetically quiet days over the period 1965-2014 for Beijing, Moscow and Tokyo neutron monitoring stations located at different cut off rigidity. The amplitude of first harmonic remains high for low cutoff rigidity as compared to high cutoff rigidity on quiet days. The diurnal amplitude significantly decreases during solar activity minimum years. The diurnal time of maximum significantly shifts to an earlier time as compared to the corotational direction having different cutoff rigidities. The time of maximum for first harmonic significantly shifts towards later hours and for second harmonic it shifts towards earlier hours at low cutoff rigidity station as compared to the high cut off rigidity station on quiet days. The amplitude of second/third harmonics shows a good positive correlation with solar wind velocity, while the others (i.e. amplitude and phase) have no significant correlation on quiet days. The amplitude and direction of the anisotropy on quiet days does not show any significant dependence on high-speed solar wind streams for these neutron monitoring stations of different cutoff rigidity threshold. Keywords: cosmic ray, cut off rigidity, quiet days, harmonics, amplitude, phase.

  4. Reconfigurable Processing Module

    NASA Technical Reports Server (NTRS)

    Somervill, Kevin; Hodson, Robert; Jones, Robert; Williams, John

    2005-01-01

    To accommodate a wide spectrum of applications and technologies, NASA s Exploration System's Missions Directorate has called for reconfigurable and modular technologies to support future missions to the moon and Mars. In response, Langley Research Center is leading a program entitled Reconfigurable Scaleable Computing (RSC) that is centered on the development of FPGA-based computing resources in a stackable form factor. This paper details the architecture and implementation of the Reconfigurable Processing Module (RPM), which is the key element of the RSC system. The RPM is an FPGA-based, space-qualified printed circuit assembly leveraging terrestrial/commercial design standards into the space applications domain. The form factor is similar to, and backwards compatible with, the PCI-104 standard utilizing only the PCI interface. The size is expanded to accommodate the required functionality while still better than 30% smaller than a 3U CompactPCI(TradeMark)card and without the overhead of the backplane. The architecture is built around two FPGA devices, one hosting PCI and memory interfaces, and another hosting mission application resources; both of which are connected with a high-speed data bus. The PCI interface FPGA provides access via the PCI bus to onboard SDRAM, flash PROM, and the application resources; both configuration management as well as runtime interaction. The reconfigurable FPGA, referred to as the Application FPGA - or simply "the application" - is a radiation-tolerant Xilinx Virtex-4 FX60 hosting custom application specific logic or soft microprocessor IP. The RPM implements various SEE mitigation techniques including TMR, EDAC, and configuration scrubbing of the reconfigurable FPGA. Prototype hardware and formal modeling techniques are used to explore the performability trade space. These models provide a novel way to calculate quality-of-service performance measures while simultaneously considering fault-related behavior due to SEE soft errors.

  5. A molecular modelling approach to understand the effect of co-evolutionary mutations (V344M, I354L) identified in the PB2 subunit of influenza A 2009 pandemic H1N1 virus on m7GTP ligand binding.

    PubMed

    Bhoye, Dipali; Behera, Abhisek Kumar; Cherian, Sarah S

    2016-08-01

    The cap binding domain of the polymerase basic 2 (PB2) subunit of influenza polymerases plays a critical role in mediating the 'cap-snatching' mechanism by binding the 5' cap of host pre-mRNAs during viral mRNA transcription. Monitoring variations in the PB2 protein is thus vital for evaluating the pathogenic potential of the virus. Based on selection pressure analysis of PB2 gene sequences of the pandemic H1N1 (pH1N1) viruses of the period 2009-2014, we identified a site, 344V/M, in the vicinity of the cap binding pocket showing evidence of adaptive evolution and another co-evolving residue, 354I/L, in close vicinity. Modelling of the three-dimensional structure of the pH1N1 PB2 cap binding domain, docking of the pre-mRNA cap analogue m7GTP and molecular dynamics simulation studies of the docked complexes performed for four PB2 variants observed showed that the complex possessing V344M with I354L possessed better ligand binding affinity due to additional hydrogen bond contacts between m7GTP and the key residues His432 and Arg355 that was attributed to a displacement of the 424 loop and a flip of the side chain of Arg355, respectively. The co-evolutionary mutations identified (V344M, I354L) were found to be established in the PB2 gene of the pH1N1 viral population over the period 2010-2014. The study demonstrates the molecular basis for the enhanced m7GTP ligand binding affinity with the 344M-354L synergistic combination in PB2. Furthermore, the insight gained into understanding the molecular mechanism of cap binding in pH1N1 viruses may be useful for designing novel drugs targeting the PB2 cap binding domain. PMID:27154164

  6. Polarization Independent Electro-Optic Modulator

    NASA Technical Reports Server (NTRS)

    Yao, Xiao-Tian Steve (Inventor)

    1997-01-01

    A polarization insensitive electro-optic modulator is constructed by providing a polarization beamsplitter to separate an incoming light beam into two orthogonally plane polarized beams. Each of the polarized beams passes through a separate electro-optic modulator where each beam is modulated by the same data signal. After modulation the beams are combined to yield a modulated beam having modulated components that are orthogonally polarized. Not only is this device insensitive to changes in polarization of the input beam, the final modulated beam can be detected by optical receivers without regard to polarization alignment of the modulated beam and the receiver.

  7. Phase modulating the Urbana radar

    NASA Technical Reports Server (NTRS)

    Herrington, L. J., Jr.; Bowhill, S. A.

    1983-01-01

    The design and operation of a switched phase modulation system for the Urbana Radar System are discussed. The system is implemented and demonstrated using a simple procedure. The radar system and circuits are described and analyzed.

  8. Ultrasound-modulated bioluminescence tomography

    NASA Astrophysics Data System (ADS)

    Bal, Guillaume; Schotland, John C.

    2014-03-01

    We propose a method to reconstruct the density of a luminescent source in a highly scattering medium from ultrasound-modulated optical measurements. Our approach is based on the solution to a hybrid inverse source problem for the diffusion equation.

  9. Vacuum lamination of photovoltaic modules

    NASA Technical Reports Server (NTRS)

    Burger, D. R.

    1982-01-01

    Vacuum lamination of terrestrial photovoltaic modules is a new high volume process requiring new equipment and newly develop materials. Equipment development, materials research, and some research in related fields and testing methods are discussed.

  10. Flip-Flop Digital Modulator

    NASA Technical Reports Server (NTRS)

    Eno, R. F.

    1984-01-01

    Clock switched on and off in response to data signal. Flip-flop modulator generates square-wave carrier frequency that is half clock frequency and turns carrier on and off. Final demodulator output logical inverse of data input.

  11. An Embedded Reconfigurable Logic Module

    NASA Technical Reports Server (NTRS)

    Tucker, Jerry H.; Klenke, Robert H.; Shams, Qamar A. (Technical Monitor)

    2002-01-01

    A Miniature Embedded Reconfigurable Computer and Logic (MERCAL) module has been developed and verified. MERCAL was designed to be a general-purpose, universal module that that can provide significant hardware and software resources to meet the requirements of many of today's complex embedded applications. This is accomplished in the MERCAL module by combining a sub credit card size PC in a DIMM form factor with a XILINX Spartan I1 FPGA. The PC has the ability to download program files to the FPGA to configure it for different hardware functions and to transfer data to and from the FPGA via the PC's ISA bus during run time. The MERCAL module combines, in a compact package, the computational power of a 133 MHz PC with up to 150,000 gate equivalents of digital logic that can be reconfigured by software. The general architecture and functionality of the MERCAL hardware and system software are described.

  12. Residential Demand Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Model Documentation - Documents the objectives, analytical approach, and development of the National Energy Modeling System (NEMS) Residential Sector Demand Module. The report catalogues and describes the model assumptions, computational methodology, parameter estimation techniques, and FORTRAN source code.

  13. Industrial Demand Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Documents the objectives, analytical approach, and development of the National Energy Modeling System (NEMS) Industrial Demand Module. The report catalogues and describes model assumptions, computational methodology, parameter estimation techniques, and model source code.

  14. Renewable Fuels Module - NEMS Documentation

    EIA Publications

    2014-01-01

    This report documents the objectives, analytical approach, and design of the National Energy Modeling System (NEMS) Renewable Fuels Module (RFM) as it relates to the production of the Annual Energy Outlook forecasts.

  15. Photovoltaic Module Qualification Plus Testing

    SciTech Connect

    Kurtz, S.; Wohlgemuth, J.; Kempe, M.; Bosco, N.; Hacke, P.; Jordan, D.; Miller, D. C.; Silverman, T. J.; Phillips, N.; Earnest, T.; Romero, R.

    2013-12-01

    This report summarizes a set of test methods that are in the midst of being incorporated into IEC 61215 for certification of a module design or other tests that go beyond certification to establish bankability.

  16. Solar cell module lamination process

    DOEpatents

    Carey, Paul G.; Thompson, Jesse B.; Aceves, Randy C.

    2002-01-01

    A solar cell module lamination process using fluoropolymers to provide protection from adverse environmental conditions and thus enable more extended use of solar cells, particularly in space applications. A laminate of fluoropolymer material provides a hermetically sealed solar cell module structure that is flexible and very durable. The laminate is virtually chemically inert, highly transmissive in the visible spectrum, dimensionally stable at temperatures up to about 200.degree. C. highly abrasion resistant, and exhibits very little ultra-violet degradation.

  17. Compact magnetic energy storage module

    DOEpatents

    Prueitt, M.L.

    1994-12-20

    A superconducting compact magnetic energy storage module in which a plurality of superconducting toroids, each having a toroidally wound superconducting winding inside a poloidally wound superconducting winding, are stacked so that the flow of electricity in each toroidally wound superconducting winding is in a direction opposite from the direction of electrical flow in other contiguous superconducting toroids. This allows for minimal magnetic pollution outside of the module. 4 figures.

  18. Compact magnetic energy storage module

    DOEpatents

    Prueitt, Melvin L.

    1994-01-01

    A superconducting compact magnetic energy storage module in which a plurality of superconducting toroids, each having a toroidally wound superconducting winding inside a poloidally wound superconducting winding, are stacked so that the flow of electricity in each toroidally wound superconducting winding is in a direction opposite from the direction of electrical flow in other contiguous superconducting toroids. This allows for minimal magnetic pollution outside of the module.

  19. Electricity Market Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Documents the Electricity Market Module as it was used for the Annual Energy Outlook 2013. The Electricity Market Module (EMM) is the electricity supply component of the National Energy Modeling System (NEMS). The EMM represents the generation, transmission, and pricing of electricity. It consists of four submodules: the Electricity Capacity Planning (ECP) Submodule, the Electricity Fuel Dispatch (EFD) Submodule, the Electricity Finance and Pricing (EFP) Submodule, and the Electricity Load and Demand (ELD) Submodule.

  20. Intelligent spacecraft module

    NASA Astrophysics Data System (ADS)

    Oungrinis, Konstantinos-Alketas; Liapi, Marianthi; Kelesidi, Anna; Gargalis, Leonidas; Telo, Marinela; Ntzoufras, Sotiris; Paschidi, Mariana

    2014-12-01

    The paper presents the development of an on-going research project that focuses on a human-centered design approach to habitable spacecraft modules. It focuses on the technical requirements and proposes approaches on how to achieve a spatial arrangement of the interior that addresses sufficiently the functional, physiological and psychosocial needs of the people living and working in such confined spaces that entail long-term environmental threats to human health and performance. Since the research perspective examines the issue from a qualitative point of view, it is based on establishing specific relationships between the built environment and its users, targeting people's bodily and psychological comfort as a measure toward a successful mission. This research has two basic branches, one examining the context of the system's operation and behavior and the other in the direction of identifying, experimenting and formulating the environment that successfully performs according to the desired context. The latter aspect is researched upon the construction of a scaled-model on which we run series of tests to identify the materiality, the geometry and the electronic infrastructure required. Guided by the principles of sensponsive architecture, the ISM research project explores the application of the necessary spatial arrangement and behavior for a user-centered, functional interior where the appropriate intelligent systems are based upon the existing mechanical and chemical support ones featured on space today, and especially on the ISS. The problem is set according to the characteristics presented at the Mars500 project, regarding the living quarters of six crew-members, along with their hygiene, leisure and eating areas. Transformable design techniques introduce spatial economy, adjustable zoning and increased efficiency within the interior, securing at the same time precise spatial orientation and character at any given time. The sensponsive configuration is

  1. Russian Docking Module is lowered

    NASA Technical Reports Server (NTRS)

    1995-01-01

    The Russian-built Docking Module (DM) is lowered for installation into the payload bay of the Space Shuttle Orbiter Atlantis while the spaceplane is in Orbiter Processing Facility bay 2. The module will fly as a primary payload on the second Space Shuttle/Mir space station docking mission, STS-74, which is now scheduled for liftoff in the fall of 1995. During the mission, the module will first be attached with the orbiter's robot arm to the Orbiter Docking System (ODS) in the payload bay of the orbiter Atlantis and then be docked with the Mir. When Atlantis undocks from the Mir, it will leave the new docking module permanently attached to the space station for use during future Shuttle Mir docking missions. The new module will simplify future Shuttle linkups with Mir by improving orbiter clearances when it serves as a bridge between the two space vehicles. The white structures attached to the module's sides are solar panels that will be attached to the Mir after the conclusion of the STS-74 mission.

  2. Wide-range voltage modulation

    SciTech Connect

    Rust, K.R.; Wilson, J.M.

    1992-06-01

    The Superconducting Super Collider`s Medium Energy Booster Abort (MEBA) kicker modulator will supply a current pulse to the abort magnets which deflect the proton beam from the MEB ring into a designated beam stop. The abort kicker will be used extensively during testing of the Low Energy Booster (LEB) and the MEB rings. When the Collider is in full operation, the MEBA kicker modulator will abort the MEB beam in the event of a malfunction during the filling process. The modulator must generate a 14-{mu}s wide pulse with a rise time of less than 1 {mu}s, including the delay and jitter times. It must also be able to deliver a current pulse to the magnet proportional to the beam energy at any time during ramp-up of the accelerator. Tracking the beam energy, which increases from 12 GeV at injection to 200 GeV at extraction, requires the modulator to operate over a wide range of voltages (4 kV to 80 kV). A vacuum spark gap and a thyratron have been chosen for test and evaluation as candidate switches for the abort modulator. Modulator design, switching time delay, jitter and pre-fire data are presented.

  3. 77 FR 54787 - Airworthiness Directives; M7 Aerospace LLC Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26...-TC, SA227-AC (C-26A), SA227-BC (C-26A), SA227-CC, SA227-DC (C-26B), SA227-AT, and SA227-TT airplanes..., SA226-T(B), SA226-TC, SA227-AC (C-26A), SA227-BC (C-26A), SA227-CC, SA227-DC (C-26B), SA227-AT,...

  4. 78 FR 77618 - Airworthiness Directives; M7 Aerospace LLC Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-24

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will..., SA226-T, SA226-T(B), SA226-TC, SA227- AC (C-26A), SA227-AT, SA227-BC (C-26A), SA227-CC, SA227-DC (C-26B...), SA227-AT, SA227-BC (C-26A), SA227-CC, SA227-DC (C-26B), SA227-TT, SA26-AT, and SA26-T airplanes,...

  5. 76 FR 50881 - Airworthiness Directives; M7 Aerospace LP Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... the control cable alongside the camera system installed in the center of the cabin. AD 87-02-02 (52 FR... Executive Order 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... ADs AD 87-02-02 (52 FR 2511, January 23, 1987) requires repetitive inspection or replacement of...

  6. Health Occupations Module. The Integumentary System.

    ERIC Educational Resources Information Center

    Temple Univ., Philadelphia, PA. Div. of Vocational Education.

    This module on the integumentary system is one of eight modules designed for individualized instruction in health occupations education programs at both the secondary and postsecondary levels. This module contains an introduction to the module topic, objectives (e.g., list and describe the types of glands formed in the skin, and explain the…

  7. Contrast adaptation to luminance and brightness modulations.

    PubMed

    Nagai, Takehiro; Nakayama, Kazuki; Kawashima, Yuki; Yamauchi, Yasuki

    2016-03-01

    Perceptual brightness and color contrast decrease after seeing a light temporally modulating along a certain direction in a color space, a phenomenon known as contrast adaptation. We investigated whether contrast adaptation along the luminance direction arises from modulation of luminance signals or apparent brightness signals. The stimulus consisted of two circles on a gray background presented on a CRT monitor. In the adaptation phase, the luminance and chromaticity of one circle were temporally modulated, while the other circle was kept at a constant luminance and color metameric with an equal-energy white. We employed two types of temporal modulations, namely, in luminance and brightness. Chromaticity was sinusoidally modulated along the L-M axis, leading to dissociation between luminance and brightness (the Helmholtz-Kohlrausch effect). In addition, luminance modulation was minimized in the brightness modulation, while brightness modulation was minimized in the luminance modulation. In the test phase, an asymmetric matching method was used to measure the magnitude of contrast adaptation for both modulations. Our results showed that, although contrast adaptation along the luminance direction occurred for both modulations, contrast adaptation for luminance modulation was significantly stronger than that for the brightness modulation regardless of the temporal frequency of the adaptation modulation. These results suggest that luminance modulation is more influential in contrast adaptation than brightness modulation.

  8. Laser frequency modulator for modulating a laser cavity

    DOEpatents

    Erbert, Gaylen V.

    1992-01-01

    The present invention relates to a laser frequency modulator for modulating a laser cavity. It is known in the prior art to utilize a PZT (piezoelectric transducer) element in combination with a mirror to change the cavity length of a laser cavity (which changes the laser frequency). Using a PZT element to drive the mirror directly is adequate at frequencies below 10 kHz. However, in high frequency applications (100 kHz and higher) PZT elements alone do not provide a sufficient change in the cavity length. The present invention utilizes an ultrasonic concentrator with a PZT element and mirror to provide modulation of the laser cavity. With an ultrasonic concentrator, the mirror element at the end of a laser cavity can move at larger amplitudes and higher frequencies.

  9. Why the New Madrid earthquakes are M 7–8 and the Charleston earthquake is ∼M 7

    USGS Publications Warehouse

    Cramer, Chris H.; Boyd, Oliver

    2014-01-01

    Estimates of magnitudes of large historical earthquakes are an essential input to and can seriously affect seismic‐hazard estimates. The earthquake‐intensity observations, modified Mercalli intensities (MMI), and assigned magnitudes Mof the 1811–1812 New Madrid events have been reinterpreted several times in the last decade and have been a source of controversy in making seismic‐hazard estimates in the central United States. Observations support the concept that the larger the earthquake, the greater the maximum‐felt distance. For the same crustal attenuation and local soil conditions, magnitude should be the main influence on intensity values at large distances. We apply this concept by comparing the mean MMI at distances of 600–1200 km for each of the four largest New Madrid 1811–1812 earthquakes, the 1886 Charleston, South Carolina, earthquake, the 1929 M 7.2 Grand Banks earthquake, and the 2001M 7.6 Bhuj, India, earthquake. We fit the intensity observations using the form MMI=A+C×dist−0.8×log(dist) to better define intensity attenuation in eastern North America (ENA). The intensity attenuation in cratonic India differs from ENA and is corrected to ENA using both the above estimate and published intensity relations. We evaluate source, marine geophysical, Q, and stress‐drop information, as well as a 1929 Milne–Shaw record at Chicago to confirm that the 1929 Grand Banks earthquake occurred in ENA crust. Our direct comparison of mean intensities beyond 600 km suggests M 7.5, 7.3, 7.7, and 6.9 for the three New Madrid 1811–1812 mainshocks and the largest aftershock and M 7.0 for the 1886 Charleston, South Carolina, earthquake, with an estimated uncertainty of 0.3 units at the 95% confidence level (based on a Monte Carlo analysis). Our mean New Madrid and Charleston mainshock magnitudes are similar to those of Bakun and Hopper (2004) and are much higher than those of Hough and Page (2011) for New Madrid.

  10. Whole Module Offgas Test Report: Space-X Dragon Module

    NASA Technical Reports Server (NTRS)

    James, John T.

    2012-01-01

    Between 7 April and 11 April 2012 a chemist from the JSC Toxicology Group acquired samples of air in 500 ml evacuated canisters from the sealed Dragon Module at the Space-X facility at KSC. Three samples were taken of facility air (two before the test and one after the test), and a total of 9 samples were taken from the sealed module in triplicate at the following times: 0 hours, 48 hours, and 96 hours. The module contained 470 kg, which was 100% of the mass to be launched. Analytical data contained in the Toxicology Group Report (attached) show that the ambient facility air was clean except for almost 9 milligrams per cubic meter of isopropanol (IPA) in the sample taken at the end of the test. Space-X must ensure that IPA is not introduced into the module before it is sealed for launch. Other minor contaminants in the ambient air included the following: perfluoro(2-methyl)pentane and hexamethylcyclotrisiloxane. The first-acquired samples of each triplicate from the module were not analyzed. Analyses of pairs of samples that were taken during the test show excellent agreement between the pairs and a linear increase in the T-values during the 4 days of the test (figure below). The rate of increase averaged 0.124 T units per day. If the time from last purge of the module on the ground to crew first entry on orbit is 10 days, then the T value at first entry should be less than 1.2 units, which is well below the criterion of 3.0 for consideration of additional protection of the crew from offgas products. The primary contributors were as follows: trimethylsilanol (0.057), fluorotrimethylsilane (0.047), acetaldehyde (0.004), hexamethylcyclopentasiloxane (0.003), and toluene (0.002).

  11. Apparatus for encapsulating a photovoltaic module

    DOEpatents

    Albright, Scot P.; Dugan, Larry M.

    1995-10-24

    The subject inventions concern various photovoltaic module designs to protect the module from horizontal and vertical impacts and degradation of solar cell efficiency caused by moisture. In one design, a plurality of panel supports that are positioned adjacent to the upper panel in a photovoltaic module absorb vertical forces exerted along an axis perpendicular to the upper panel. Other designs employ layers of glass and tempered glass, respectively, to protect the module from vertical impacts. A plurality of button-shaped channels is used around the edges of the photovoltaic module to absorb forces applied to the module along an axis parallel to the module and direct moisture away from the module that could otherwise penetrate the module and adversely affect the cells within the module. A spacer is employed between the upper and lower panels that has a coefficient of thermal expansion substantially equivalent to the coefficient of thermal expansion of at least one of the panels.

  12. Automated solar module assembly line

    NASA Technical Reports Server (NTRS)

    Bycer, M.

    1980-01-01

    The solar module assembly machine which Kulicke and Soffa delivered under this contract is a cell tabbing and stringing machine, and capable of handling a variety of cells and assembling strings up to 4 feet long which then can be placed into a module array up to 2 feet by 4 feet in a series of parallel arrangement, and in a straight or interdigitated array format. The machine cycle is 5 seconds per solar cell. This machine is primarily adapted to 3 inch diameter round cells with two tabs between cells. Pulsed heat is used as the bond technique for solar cell interconnects. The solar module assembly machine unloads solar cells from a cassette, automatically orients them, applies flux and solders interconnect ribbons onto the cells. It then inverts the tabbed cells, connects them into cell strings, and delivers them into a module array format using a track mounted vacuum lance, from which they are taken to test and cleaning benches prior to final encapsulation into finished solar modules. Throughout the machine the solar cell is handled very carefully, and any contact with the collector side of the cell is avoided or minimized.

  13. NEMS integrating module documentation report

    SciTech Connect

    Not Available

    1993-12-14

    The National Energy Modeling System (NEMS) is a computer modeling system that produces a general equilibrium solution for energy supply and demand in the US energy markets. The model achieves a supply and demand balance in the end-use demand regions, defined as the nine Census Divisions, by solving for the prices of each energy type such that the quantities producers are willing to supply equal the quantities consumers wish to consume. The system reflects market economics, industry structure, and energy policies and regulations that influence market behavior. The NEMS Integrating Module is the central integrating component of a complex modeling system. As such, a thorough understanding of its role in the modeling process can only be achieved by placing it in the proper context with respect to the other modules. To that end, this document provides an overview of the complete NEMS model, and includes brief descriptions of the modules with which the Integrating Module interacts. The emphasis and focus, however, is on the structure and function of the Integrating Module of NEMS.

  14. Compact nanomechanical plasmonic phase modulators

    SciTech Connect

    Dennis, B. S.; Haftel, M. I.; Czaplewski, D. A.; Lopez, D.; Blumberg, G.; Aksyuk, V. A.

    2015-03-30

    Highly confined optical energy in plasmonic devices is advancing miniaturization in photonics. However, for mode sizes approaching ≈10 nm, the energy increasingly shifts into the metal, raising losses and hindering active phase modulation. Here, we propose a nanoelectromechanical phase-modulation principle exploiting the extraordinarily strong dependence of the phase velocity of metal–insulator–metal gap plasmons on dynamically variable gap size. We experimentally demonstrate a 23-μm-long non-resonant modulator having a 1.5π rad range, with 1.7 dB excess loss at 780 nm. Analysis shows that by simultaneously decreasing the gap, length and width, an ultracompact-footprint π rad phase modulator can be realized. This is achieved without incurring the extra loss expected for plasmons confined in a decreasing gap, because the increasing phase-modulation strength from a narrowing gap offsets rising propagation losses. Such small, high-density electrically controllable components may find applications in optical switch fabrics and reconfigurable plasmonic optics.

  15. CMOTT Turbulence Module for NPARC

    NASA Technical Reports Server (NTRS)

    Zhu, J.; Shih, T.-H.

    1997-01-01

    This is a user's manual of the CMOTT turbulence module, version 2.0, developed for the NPARC code. The module is written in a self-contained manner so that the user can use any turbulence model in the module without concern as to how it is implemented and solved. Three two-equation turbulence models have been built into the module: Chien, Shih-Lumley and CMOTT models, and all of them have both the low Reynolds number and wall function options. Unlike Chien's model, both the Shih-Lumley and CMOTT models do not involve the dimensionless wall distance y(sup +) in the low Reynolds number approach, an advantage for separated flow calculations. The Van Driest transformation is used so that the wall functions can be applied to both incompressible and compressible flows. The manual gives the details of the turbulence models used and their numerical implementation. It also gives two application examples, one for subsonic and the other for transonic flow, for demonstration. The module can be easily linked to the NPARC code for practical applications.

  16. Joint Acoustic and Modulation Frequency

    NASA Astrophysics Data System (ADS)

    Atlas, Les; Shamma, Shihab A.

    2003-12-01

    There is a considerable evidence that our perception of sound uses important features which is related to underlying signal modulations. This topic has been studied extensively via perceptual experiments, yet there are few, if any, well-developed signal processing methods which capitalize on or model these effects. We begin by summarizing evidence of the importance of modulation representations from psychophysical, physiological, and other sources. The concept of a two-dimensional joint acoustic and modulation frequency representation is proposed. A simple single sinusoidal amplitude modulator of a sinusoidal carrier is then used to illustrate properties of an unconstrained and ideal joint representation. Added constraints are required to remove or reduce undesired interference terms and to provide invertibility. It is then noted that the constraints would also apply to more general and complex cases of broader modulation and carriers. Applications in single-channel speaker separation and in audio coding are used to illustrate the applicability of this joint representation. Other applications in signal analysis and filtering are suggested.

  17. Quench Module Insert (QMI) and the Diffusion Module Insert (DMI) Furnace Development

    NASA Technical Reports Server (NTRS)

    Crouch, Myscha; Carswell, William; Farmer, Jeff; Rose, Fred; Tidwell, Paul

    2000-01-01

    This paper presents, in viewgraph form, QMI (Quench Module Insert) and DMI (Diffusion Module Insert) furnace development. The topics include: 1) Furnace Module in Rack; 2) Quench Module Insert; 3) QMI in MSL Core; 4) Diffusion Module Insert; 5) QMI; and 6) QMI Development and Testing.

  18. Command and Service Module Communications

    NASA Technical Reports Server (NTRS)

    Interbartolo, Michael

    2009-01-01

    This viewgraph presentation examines Command and Service Module (CSM) Communications. The communication system's capabilities are defined, including CSM-Earth, CSM-Lunar Module and CSM-Extravehicular crewman communications. An overview is provided for S-band communications, including data transmission and receiving rates, operating frequencies and major system components (pre-modulation processors, unified S-band electronics, S-band power amplifier and S-band antennas). Additionally, data transmission rates, operating frequencies and the capabilities of VHF communications are described. Major VHF components, including transmitters and receivers, and the VHF multiplexer and antennas are also highlighted. Finally, communications during pre-launch, ascent, in-flight and entry are discussed. Overall, the CSM communication system was rated highly by flight controllers and crew. The system was mostly autonomous for both crew and flight controllers and no major issues were encountered during flight.

  19. Unity connecting module in SSPF

    NASA Technical Reports Server (NTRS)

    1998-01-01

    In the Space Station Processing Facility, the Unity connecting module, part of the International Space Station, is shown with Pressurized Mating Adapters 1 (left) and 2 (right) attached. Unity is scheduled to undergo testing of the common berthing mechanism to which other space station elements will dock. Unity is the primary payload on mission STS-88, targeted to launch Dec. 3, 1998. Other testing includes the Pad Demonstration Test to verify the compatibility of the module with the Space Shuttle as well as the ability of the astronauts to send and receive commands to Unity from the flight deck of the orbiter. Unity is expected to be ready for installation into the payload canister on Oct. 25, and transported to Launch Pad 39-A on Oct. 27. The Unity will be mated to the Russian-built Zarya control module which should already be in orbit at that time.

  20. Allosteric Modulation of Chemoattractant Receptors

    PubMed Central

    Allegretti, Marcello; Cesta, Maria Candida; Locati, Massimo

    2016-01-01

    Chemoattractants control selective leukocyte homing via interactions with a dedicated family of related G protein-coupled receptor (GPCR). Emerging evidence indicates that the signaling activity of these receptors, as for other GPCR, is influenced by allosteric modulators, which interact with the receptor in a binding site distinct from the binding site of the agonist and modulate the receptor signaling activity in response to the orthosteric ligand. Allosteric modulators have a number of potential advantages over orthosteric agonists/antagonists as therapeutic agents and offer unprecedented opportunities to identify extremely selective drug leads. Here, we resume evidence of allosterism in the context of chemoattractant receptors, discussing in particular its functional impact on functional selectivity and probe/concentration dependence of orthosteric ligands activities. PMID:27199992

  1. Common modules: A success story

    NASA Astrophysics Data System (ADS)

    Morrow, Walter B., Jr.

    1988-06-01

    During the late 1960s and early 1970s, the U.S. military found far-infrared or thermal imaging systems very useful as night vision devices because of ready application as troop-carried, combat vehicle-mounted, and aircraft-mounted systems. The rapid proliferation of applications posed a serious financial problem, yet clearly solved critical needs. The development and acquisition process for each system's development, purchase, and maintenance was both lengthy and expensive. Therefore, an investigative committee was formed in 1971 within the Center for Night Vision and Electro-Optics to analyze the problems and develop the design concept for Common Modules. This report focuses on the detector modules and develops cost benefits accrued to date and expected to accrue in the Army's future as a result of common module development and implementation.

  2. Photovoltaic module bypass diode encapsulation

    NASA Technical Reports Server (NTRS)

    Shepard, N. J., Jr.

    1983-01-01

    The design and processing techniques necessary to incorporate bypass diodes within the module encapsulant are presented. The Semicon PN junction diode cells were selected. Diode junction to heat spreader thermal resistance measurements, performed on a variety of mounted diode chip types and sizes, have yielded values which are consistently below 1 deg C per watt, but show some instability when thermally cycled over the temperature range from -40 to 150 deg C. Three representative experimental modules, each incorporating integral bypass diode/heat spreader assemblies of various sizes, were designed. Thermal testing of these modules enabled the formulation of a recommended heat spreader plate sizing relationship. The production cost of three encapsulated bypass diode/heat spreader assemblies were compared with similarly rated externally mounted packaged diodes. It is concluded that, when proper designed and installed, these bypass diode devices will improve the overall reliability of a terrestrial array over a 20 year design lifetime.

  3. Advanced infrared laser modulator development

    NASA Technical Reports Server (NTRS)

    Cheo, P. K.; Wagner, R.; Gilden, M.

    1984-01-01

    A parametric study was conducted to develop an electrooptic waveguide modulator for generating continuous tunable sideband power from an infrared CO2 laser. Parameters included were the waveguide configurations, microstrip dimensions device impedance, and effective dielectric constants. An optimum infrared laser modulator was established and was fabricated. This modulator represents the state-of-the-art integrated optical device, which has a three-dimensional topology to accommodate three lambda/4 step transformers for microwave impedance matching at both the input and output terminals. A flat frequency response of the device over 20 HGz or = 3 dB) was achieved. Maximum single sideband to carrier power greater than 1.2% for 20 W microwave input power at optical carrier wavelength of 10.6 microns was obtained.

  4. A compact nanosecond pulse modulator

    NASA Astrophysics Data System (ADS)

    Sha, Jizhang; Xue, Jianchao; Qiang, Bohan

    Two circuits of nanosecond pulse modulator which generate two different width rectangular pulses respectively are described. The basic configuration of the modulator is the Marx circuit, in which avalanche transistors are used as switching devices. In order to obtain the rectangular pulses a pulse-forming network (PFN) is introduced and fitted into the Marx. A multi-parallel arrangement of the Marx is used to satisfy the broad pulse requirement. Experiments have shown that the two different width rectangular pulses which have 130 V amplitudes and 30 and 200 ns widths respectively can be obtained at a 50 ohms load. The two pulses have steep front edges (3.6 ns and 10 ns respectively) and flat tops with less than + or - 5 percent ripples. Therefore, the modulator can meet the requirements of the nanosecond pulse radar.

  5. Vanadium dioxide based plasmonic modulators.

    PubMed

    Sweatlock, Luke A; Diest, Kenneth

    2012-04-01

    Actively tunable metal-insulator-metal waveguides that employ vanadium dioxide films as the active medium are analyzed numerically. Vanadium dioxide exhibits strong contrast between the optical properties of its insulating and metallic phases. In particular, the large optical absorption in the metallic phase makes it straightforward to implement broadband attenuation modulators and switches, but this strong loss can also complicate the design of other types of devices. We present a plasmonic waveguide that functions as an index modulator with Δn > 20% at λ0 = 1,550 nm (0.80 eV), by using a thin active layer to strike a balance between maximizing index contrast while mitigating attenuation. A second device is configured as a band-stop absorption modulator, taking advantage of symmetry to selectively suppress the TM1 and TM3 modes, with relatively minimal attenuation of the TM0 and TM2 modes.

  6. Active combustion flow modulation valve

    DOEpatents

    Hensel, John Peter; Black, Nathaniel; Thorton, Jimmy Dean; Vipperman, Jeffrey Stuart; Lambeth, David N; Clark, William W

    2013-09-24

    A flow modulation valve has a slidably translating hollow armature with at least one energizable coil wound around and fixably attached to the hollow armature. The energizable coil or coils are influenced by at least one permanent magnet surrounding the hollow armature and supported by an outer casing. Lorentz forces on the energizable coils which are translated to the hollow armature, increase or decrease the flow area to provide flow throttling action. The extent of hollow armature translation depends on the value of current supplied and the direction of translation depends on the direction of current flow. The compact nature of the flow modulation valve combined with the high forces afforded by the actuator design provide a flow modulation valve which is highly responsive to high-rate input control signals.

  7. Progress of MICE RFCC Module

    SciTech Connect

    Li, D.; Bowring, D.; DeMello, A.; Gourlay, S.; Green, M.; Li, N.; Niinikoski, T.; Pan, H.; Prestemon, S.; Virostek, S.; Zisman, M.; Bross, A.; Carcagno, R.; Kashikhin, V.; Sylvester, C.; Chen, A. B.; Guo, Bin; Li, Liyi; Xu, Fengyu; Cao, Y.; Sun, S.; Wang, Li; Yin, Lixin; Luo, Tianhuan; Summers, Don; Smith, B.; Radovinsky, A.; Zhukovsky, A.; Kaplan, D.

    2012-05-20

    Recent progress on the design and fabrication of the RFCC (RF and superconducting Coupling Coil) module for the international MICE (Muon Ionization Cooling Experiment) are reported. The MICE ionization cooling channel has two RFCC modules, each having four 201- MHz normal conducting RF cavities surrounded by one superconducting coupling coil (solenoid) magnet. The magnet is designed to be cooled by three cryocoolers. Fabrication of the RF cavities is complete; preparation for the cavity electro-polishing, low power RF measurements, and tuning are in progress at Lawrence Berkeley National Laboratory (LBNL). Fabrication of the cold mass of the first coupling coil magnet has been completed in China and the cold mass arrived at LBNL in late 2011. Preparations for testing the cold mass are currently under way at Fermilab. Plans for the RFCC module assembly and integration are being developed and are described.

  8. The assess facility descriptor module

    SciTech Connect

    Jordan, S.E.; Winblad, A.; Key, B.; Walker, S.; Renis, T.; Saleh, R.

    1989-01-01

    The Facility Descriptor (Facility) module is part of the Analytic System and Software for Evaluating Safeguards and Security (ASSESS). Facility is the foundational software application in the ASSESS system for modelling a nuclear facility's safeguards and security system to determine the effectiveness against theft of special nuclear material. The Facility module provides the tools for an analyst to define a complete description of a facility's physical protection system which can then be used by other ASSESS software modules to determine vulnerability to a spectrum of insider and outsider threats. The analyst can enter a comprehensive description of the protection system layout including all secured areas, target locations, and detailed safeguards specifications. An extensive safeguard component catalog provides the reference data for calculating delay and detection performance. Multiple target locations within the same physical area may be specified, and the facility may be defined for two different operational states such as dayshift and nightshift. 6 refs., 5 figs.

  9. Solid-state membrane module

    DOEpatents

    Gordon, John Howard; Taylor, Dale M.

    2011-06-07

    Solid-state membrane modules comprising at least one membrane unit, where the membrane unit has a dense mixed conducting oxide layer, and at least one conduit or manifold wherein the conduit or manifold comprises a dense layer and at least one of a porous layer and a slotted layer contiguous with the dense layer. The solid-state membrane modules may be used to carry out a variety of processes including the separating of any ionizable component from a feedstream wherein such ionizable component is capable of being transported through a dense mixed conducting oxide layer of the membrane units making up the membrane modules. For ease of construction, the membrane units may be planar.

  10. Command module/service module reaction control subsystem assessment

    NASA Technical Reports Server (NTRS)

    Weary, D. P.

    1971-01-01

    Detailed review of component failure histories, qualification adequacy, manufacturing flow, checkout requirements and flow, ground support equipment interfaces, subsystem interface verification, protective devices, and component design did not reveal major weaknesses in the command service module (CSM) reaction control system (RCS). No changes to the CSM RCS were recommended. The assessment reaffirmed the adequacy of the CSM RCS for future Apollo missions.

  11. Doping modulated carbon nanotube synapstors for a spike neuromorphic module.

    PubMed

    Shen, Alex Ming; Kim, Kyunghyun; Tudor, Andrew; Lee, Dongwon; Chen, Yong

    2015-04-01

    A doping-modulated carbon nanotube (CNT) electronic device, called a "synapstor," emulates the function of a biological synapse. The CNT synapstor has a field-effect transistor structure with a random CNT network as its channel. An aluminium oxide (Al2 O3 ) film is deposited over half of the CNT channel in the synapstor, converting the covered part of the CNT from p-type to n-type, forming a p-n junction in the CNT channel and increasing the Schottky barrier between the n-type CNT and its metal contact. This scheme significantly improves the postsynaptic current (PSC) from the synapstor, extends the tuning range of the plasticity, and reduces the power consumption of the CNT synapstor. A spike neuromorphic module is fabricated by integrating the CNT synapstors with a Si-based "soma" circuit. Spike parallel processing, memory, and plasticity functions of the module are demonstrated. The module could potentially be integrated and scaled up to emulate a biological neural network with parallel high-speed signal processing, low power consumption, memory, and learning capabilities. PMID:25423906

  12. CAMAC 488 module: 68,000 based GPIB interface module

    SciTech Connect

    Seino, K.C.

    1985-03-01

    What kind of hardware and software should be used to interface GPIB devices with the existing computer system. One idea was to use a commercially available Multibus card, BLC 8488 from National Semiconductor, whose on-board Z80 would manage the GPIB read/write functions and handshakes. With this card, one could make a hardware system which would consist of (1) CAMAC 080, (2) Multibus crate, (3) M. Shea's M68000, (4) M080, (5) BLC 8488 and (6) memory board. And the software considered for such a hardware package was GAS, which had been an established software package for communication between the ACNET computer system and smart CAMAC modules. However, a second idea was to put everything on a two-wide CAMAC module. The author pursued the second idea and came up with a two-wide CAMAC module called C488. The author describes the hardware - block diagrams, circuit blocks, front panel and hardware tests. He also refers to the software - system, modules and applications.

  13. Monolithic 20-GHz Transmitting Module

    NASA Technical Reports Server (NTRS)

    Kascak, T.; Kaelin, G.; Gupta, A.

    1986-01-01

    20-GHz monolithic microwave/millimeter-wave integrated circuit (MMIC) with amplification and phase-shift (time-delay) capabilities developed. Use of MMIC module technology promises to make feasible development of weight- and cost-effective phased-array antenna systems, identified as major factor in achieving minimum cost and efficient use of frequency and orbital resources of future generations of communication satellite systems. Use of MMIC transmitting modules provides for relatively simple method for phase-shift control of many separate radio-frequency (RF) signals required for phased-array antenna systems.

  14. Static feed water electrolysis module

    NASA Technical Reports Server (NTRS)

    Powell, J. D.; Schubert, F. H.; Jensen, F. C.

    1974-01-01

    An advanced static feed water electrolysis module (SFWEM) and associated instrumentation for generating breathable O2 was developed. The system also generates a H2 byproduct for use in an air revitalization system for O2 recovery from metabolic CO2. Special attention was given to: (1) eliminating water feed compartment degassing, (2) eliminating need for zero gravity condenser/separators, (3) increasing current density capability, and (4) providing a self contained module so that operation is independent of laboratory instrumentation and complicated startup/shutdown procedures.

  15. Programmable Multi-Chip Module

    DOEpatents

    Kautz, David; Morgenstern, Howard; Blazek, Roy J.

    2004-11-16

    A multi-chip module comprising a low-temperature co-fired ceramic substrate having a first side on which are mounted active components and a second side on which are mounted passive components, wherein this segregation of components allows for hermetically sealing the active components with a cover while leaving accessible the passive components, and wherein the passive components are secured using a reflow soldering technique and are removable and replaceable so as to make the multi-chip module substantially programmable with regard to the passive components.

  16. Programmable multi-chip module

    DOEpatents

    Kautz, David; Morgenstern, Howard; Blazek, Roy J.

    2004-03-02

    A multi-chip module comprising a low-temperature co-fired ceramic substrate having a first side on which are mounted active components and a second side on which are mounted passive components, wherein this segregation of components allows for hermetically sealing the active components with a cover while leaving accessible the passive components, and wherein the passive components are secured using a reflow soldering technique and are removable and replaceable so as to make the multi-chip module substantially programmable with regard to the passive components.

  17. Programmable Multi-Chip Module

    DOEpatents

    Kautz, David; Morgenstern, Howard; Blazek, Roy J.

    2005-05-24

    A multi-chip module comprising a low-temperature co-fired ceramic substrate having a first side on which are mounted active components and a second side on which are mounted passive components, wherein this segregation of components allows for hermetically sealing the active components with a cover while leaving accessible the passive components, and wherein the passive components are secured using a reflow soldering technique and are removable and replaceable so as to make the multi-chip module substantially programmable with regard to the passive components.

  18. ModuleOrganizer: detecting modules in families of transposable elements

    PubMed Central

    2010-01-01

    Background Most known eukaryotic genomes contain mobile copied elements called transposable elements. In some species, these elements account for the majority of the genome sequence. They have been subject to many mutations and other genomic events (copies, deletions, captures) during transposition. The identification of these transformations remains a difficult issue. The study of families of transposable elements is generally founded on a multiple alignment of their sequences, a critical step that is adapted to transposons containing mostly localized nucleotide mutations. Many transposons that have lost their protein-coding capacity have undergone more complex rearrangements, needing the development of more complex methods in order to characterize the architecture of sequence variations. Results In this study, we introduce the concept of a transposable element module, a flexible motif present in at least two sequences of a family of transposable elements and built on a succession of maximal repeats. The paper proposes an assembly method working on a set of exact maximal repeats of a set of sequences to create such modules. It results in a graphical view of sequences segmented into modules, a representation that allows a flexible analysis of the transformations that have occurred between them. We have chosen as a demonstration data set in depth analysis of the transposable element Foldback in Drosophila melanogaster. Comparison with multiple alignment methods shows that our method is more sensitive for highly variable sequences. The study of this family and the two other families AtREP21 and SIDER2 reveals new copies of very different sizes and various combinations of modules which show the potential of our method. Conclusions ModuleOrganizer is available on the Genouest bioinformatics center at http://moduleorganizer.genouest.org PMID:20860790

  19. Environmental testing of block 3 solar cell modules. Part 1: Qualification testing of standard production modules

    NASA Technical Reports Server (NTRS)

    Griffith, J. S.

    1979-01-01

    Qualification tests of solar cell modules are described. These modules continue to show improvement over earlier type modules tested. Cell cracking and delamination are less prevalent, and interconnect problems and electrical degradation from environmental testing are now rare.

  20. Transportation Brokerage: An Instructional Module.

    ERIC Educational Resources Information Center

    Hayden, Linda

    A concept-based introduction to transportation brokerage is provided in this instructional module for undergraduate and graduate transportation-related courses for disciplines such as engineering, business, marketing, and technology. The concept of transportation brokerage is defined as an assignment of the management of a specific element of a…

  1. Project-Based Module Development.

    ERIC Educational Resources Information Center

    Meel, R. M. van

    A project management design for modularizing higher education at open universities was developed and tested. Literature in the fields of project management and development of modular curriculum materials was reviewed and used as a basis for developing a project-based approach to the process of developing modules for self-instruction. According to…

  2. Adolescence Education: Physical Aspect, Module One; Social Aspects, Module Two; Sex Roles, Module Three; Sexually Transmitted Diseases, Module Four.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific and Cultural Organization, Bangkok (Thailand). Principal Regional Office for Asia and the Pacific.

    Adolescence Education is a family life education training program designed to assist young people in their physical, social, emotional, and moral development as they prepare for adulthood, marriage, parenthood, aging, and social relationships in the context of family and society. This package consists of four individually bound modules: (1)…

  3. Rights & Responsibilities. Personnel Management Module.

    ERIC Educational Resources Information Center

    Barker, Gale; And Others

    This module on rights and responsibilities is intended to introduce the hospitality manager or supervisor to sound personnel management practices that comply with the law. The material is presented in a self-instructional format in seven sections. At the beginning of each section is a statement of the objectives that will be achieved as a result…

  4. Module/array interface study

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Several aspects of module design are evaluated, including glass superstrate and metal substrate module configurations, the potential for hail damage, light absorption in glass superstrates, the economics of glass selection, and electrical design. Also, three alternate glass superstrate module configurations are evaluated by means of finite element computer analyses. Two panel sizes, 1.2 by 2.4 m (4 by 8 ft) and 2.4 by 4.8 m are used to support three module sizes, 0.6 by 1.2 m, 1.2 by 1.2 m, and 1.2 by 2.4 m, for design loadings of + or - 1.7 kPa, + or - 2.4 kPa, and + or - 3.6 kPa. Designs and cost estimates are presented for twenty panel types and nine array configurations at each of the three design loadings. Structural cost sensitivities of combined array configurations and panel cases are presented.

  5. Can Arousal Modulate Response Inhibition?

    ERIC Educational Resources Information Center

    Weinbach, Noam; Kalanthroff, Eyal; Avnit, Amir; Henik, Avishai

    2015-01-01

    The goal of the present study was to examine if and how arousal can modulate response inhibition. Two competing hypotheses can be drawn from previous literature. One holds that alerting cues that elevate arousal should result in an impulsive response and therefore impair response inhibition. The other suggests that alerting enhances processing of…

  6. Learning Environments: An Instructional Module.

    ERIC Educational Resources Information Center

    Wiley, Ann L.

    This self-instructional module includes learner objectives, text, practice exercises and answer sheets addressing developments in research on learning environments and why instructional technologists should take the lead in applying the resulting knowledge to the design of training areas. Favorable learning environments are described in general…

  7. The Knife Machine. Module 15.

    ERIC Educational Resources Information Center

    South Carolina State Dept. of Education, Columbia. Office of Vocational Education.

    This module on the knife machine, one in a series dealing with industrial sewing machines, their attachments, and operation, covers one topic: performing special operations on the knife machine (a single needle or multi-needle machine which sews and cuts at the same time). These components are provided: an introduction, directions, an objective,…

  8. Reward Modulates Adaptations to Conflict

    ERIC Educational Resources Information Center

    Braem, Senne; Verguts, Tom; Roggeman, Chantal; Notebaert, Wim

    2012-01-01

    Both cognitive conflict (e.g. Verguts & Notebaert, 2009) and reward signals (e.g. Waszak & Pholulamdeth, 2009) have been proposed to enhance task-relevant associations. Bringing these two notions together, we predicted that reward modulates conflict-based sequential adaptations in cognitive control. This was tested combining either a single…

  9. Microcomputer Modules for Undergraduate Geography.

    ERIC Educational Resources Information Center

    Groop, Richard; And Others

    1985-01-01

    Described and evaluated are microcomputer units of instruction that were developed for use in undergraduate geography courses. Students responded favorably to the modules--"Socioeconomic Patterns,""Economic Rent,""Sampling Distribution of Sample Means,""Land Use Competition,""Data Classing,""Weather and Climate," and "Landforms." (RM)

  10. Supported Employment. Missouri LINC Module.

    ERIC Educational Resources Information Center

    Missouri Univ., Columbia. Coll. of Education.

    This module provides information on supported employment as outlined in the Rehabilitation Act Amendments of 1986, P.L. 99-506. It discusses models of supported employment; the roles of vocational rehabilitation counselors, school district personnel, and job coaches; and what school district personnel can do to prepare students for supported…

  11. Wavelength-modulated photocapacitance spectroscopy

    NASA Technical Reports Server (NTRS)

    Kamieniecki, E.; Lagowski, J.; Gatos, H. C.

    1980-01-01

    Derivative deep-level spectroscopy was achieved with wavelength-modulated photocapacitance employing MOS structures and Schottky barriers. The energy position and photoionization characteristics of deep levels of melt-grown GaAs and the Cr level in high-resistivity GaAs were determined. The advantages of this method over existing methods for deep-level spectroscopy are discussed.

  12. Clothing and Textile Student Modules.

    ERIC Educational Resources Information Center

    South Carolina State Dept. of Education, Columbia. Office of Vocational Education.

    Forty-seven performance-based instructional modules on six major topics are provided for the home economics content area of clothing and textiles. The six topics are (1) planning basics (psychological, physical, social, and behavioral aspects of clothing; elements of design; principles of design; and style and fashion in clothing), (2) buyership…

  13. Coal Market Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Documents the objectives and the conceptual and methodological approach used in the development of the National Energy Modeling System's (NEMS) Coal Market Module (CMM) used to develop the Annual Energy Outlook 2014 (AEO2014). This report catalogues and describes the assumptions, methodology, estimation techniques, and source code of CMM's two submodules. These are the Coal Production Submodule (CPS) and the Coal Distribution Submodule (CDS).

  14. Attention modulates visual size adaptation.

    PubMed

    Kreutzer, Sylvia; Fink, Gereon R; Weidner, Ralph

    2015-01-01

    The current study determined in healthy subjects (n = 16) whether size adaptation occurs at early, i.e., preattentive, levels of processing or whether higher cognitive processes such as attention can modulate the illusion. To investigate this issue, bottom-up stimulation was kept constant across conditions by using a single adaptation display containing both small and large adapter stimuli. Subjects' attention was directed to either the large or small adapter stimulus by means of a luminance detection task. When attention was directed toward the small as compared to the large adapter, the perceived size of the subsequent target was significantly increased. Data suggest that different size adaptation effects can be induced by one and the same stimulus depending on the current allocation of attention. This indicates that size adaptation is subject to attentional modulation. These findings are in line with previous research showing that transient as well as sustained attention modulates visual features, such as contrast sensitivity and spatial frequency, and influences adaptation in other contexts, such as motion adaptation (Alais & Blake, 1999; Lankheet & Verstraten, 1995). Based on a recently suggested model (Pooresmaeili, Arrighi, Biagi, & Morrone, 2013), according to which perceptual adaptation is based on local excitation and inhibition in V1, we conclude that guiding attention can boost these local processes in one or the other direction by increasing the weight of the attended adapter. In sum, perceptual adaptation, although reflected in changes of neural activity at early levels (as shown in the aforementioned study), is nevertheless subject to higher-order modulation.

  15. Market Segmentation: An Instructional Module.

    ERIC Educational Resources Information Center

    Wright, Peter H.

    A concept-based introduction to market segmentation is provided in this instructional module for undergraduate and graduate transportation-related courses. The material can be used in many disciplines including engineering, business, marketing, and technology. The concept of market segmentation is primarily a transportation planning technique by…

  16. Automated microorganism Sample Collection Module

    NASA Technical Reports Server (NTRS)

    Gall, L. S.; Graham, M. D.; Umbreit, W.

    1969-01-01

    Modified Gelman Sampler obtains representative sample of microorganism population. Proposed Sample Collection Module is based on direct inoculation of selected solid growth media encased in a cartridge at all times except during inoculation. Cartridge can be handled with no danger of contamination to sample or operator.

  17. LISA Propulsion Module Separation Study

    NASA Technical Reports Server (NTRS)

    Merkowitz, Stephen

    2004-01-01

    The Laser Interferometer Space Antenna (LISA) mission is a space-borne gravitational wave detector consisting of three spacecraft in heliocentric orbit. Each spacecraft is delivered to it operational orbit by a propulsion module. Because of the strict thermal and mass balancing requirements of LISA, the baseline mission concept requires that the propulsion module separate from the sciencecraft after delivery. The only propulsion system currently baselined for the sciencecraft are micronewton level thrusters, such as FEEP or colloid thrusters, that are used to balance the 30-40 microN of solar radiation pressure and provide the drag-free and attitude control of the spacecraft. Due to these thrusters limited authority, the separation of the propulsion module from the sciencecraft must be well controlled to not induce a large tip-off rotation of the sciencecraft. We present here the results of a design study of the propulsion module separation system that is shown to safely deliver the LISA sciencecraft to its final operational orbit.

  18. Polynomial Beam Element Analysis Module

    SciTech Connect

    Ning, S. Andrew

    2013-05-01

    pBEAM (Polynomial Beam Element Analysis Module) is a finite element code for beam-like structures. The methodology uses Euler? Bernoulli beam elements with 12 degrees of freedom (3 translation and 3 rotational at each end of the element).

  19. Commercial Demand Module - NEMS Documentation

    EIA Publications

    2014-01-01

    Documents the objectives, analytical approach and development of the National Energy Modeling System (NEMS) Commercial Sector Demand Module. The report catalogues and describes the model assumptions, computational methodology, parameter estimation techniques, model source code, and forecast results generated through the synthesis and scenario development based on these components.

  20. View of docked Apollo 9 Command/Service Module and Lunar Module

    NASA Technical Reports Server (NTRS)

    1969-01-01

    View of the docked Apollo 9 Command/Service Modules and Lunar Module, with Earth in the background, during Astronaut David R. Scott's stand-up extravehicular activity, on the fouth day of the Apollo 9 earth-orbital mission. Scott, command module pilot, is standing in the open hatch of the Command module. Astronaut Russell L. Schweickart, lunar module pilot, took this photograph of Scott from the porch of the Lunar Module.