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Sample records for multiplexed readout system

  1. Enhancements to a Superconducting Quantum Interference Device (SQUID) Multiplexer Readout and Control System

    NASA Technical Reports Server (NTRS)

    Forgione, J.; Benford, D. J.; Buchanan, E. D.; Moseley, S. H.; Rebar, J.; Shafer, R. A.

    2004-01-01

    Far-infrared detector arrays such as the 16x32 superconducting bolometer array for the SAFIRE instrument (flying on the SOFIA airborne observatory) require systems of readout and control electronics to provide translation between a user-driven, digital PC and the cold, analog world of the cryogenic detector. In 2001, the National Institute of Standards and Technology (NIST) developed their Mark III electronics for purposes of control and readout of their 1x32 SQUID Multiplexer chips. We at NASA s Goddard Space Flight Center acquired a Mark 111 system and subsequently designed upgrades to suit our and our collaborators purposes. We developed an arbitrary, programmable multiplexing system that allows the user to cycle through rows in a SQUID array in an infinite number of combinations. We provided hooks in the Mark III system to allow readout of signals from outside the Mark 111 system, such as telescope status information. Finally, we augmented the heart of the system with a new feedback algorithm implementation, flexible diagnostic tools, and informative telemetry.

  2. Development of Frequency-Division Multiplexing Readout System for Large-Format TES X-ray Microcalorimeter Arrays

    NASA Astrophysics Data System (ADS)

    Sakai, K.; Yamamoto, R.; Takei, Y.; Mitsuda, K.; Yamasaki, N. Y.; Hidaka, M.; Nagasawa, S.; Kohjiro, S.; Miyazaki, T.

    2016-07-01

    We are developing the frequency-division multiplexing (FDM) readout system aimed to realize the 400-pixel transition edge sensor (TES) microcalorimeter array for the DIOS mission as well as large-format arrays with more than a thousand of TES for future space missions such as the ATHENA mission. The developed system consists of the low-power superconducting quantum interference device (SQUID), the digital FDM electronics, and the analog front-end to bridge the SQUID and the digital electronics. Using the developed readout system, we performed a TES readout experiment and succeeded to multiplex four TES signals with the single-staged cryogenic setup. We have experienced two issues during the experiment: an excess noise and crosstalk. The brief overview of the developed system and the details, results, and issues of the TES multiplexing readout experiment is discussed.

  3. Planar Lithographed Superconducting LC Resonators for Frequency-Domain Multiplexed Readout Systems

    NASA Astrophysics Data System (ADS)

    Rotermund, K.; Barch, B.; Chapman, S.; Hattori, K.; Lee, A.; Palaio, N.; Shirley, I.; Suzuki, A.; Tran, C.

    2016-07-01

    Cosmic microwave background (CMB) polarization experiments are increasing the number of transition edge sensor (TES) bolometers to increase sensitivity. In order to maintain low thermal loading of the sub-Kelvin stage, the frequency-domain multiplexing (FDM) factor has to increase accordingly. FDM is achieved by placing TES bolometers in series with inductor-capacitor (LC) resonators, which select the readout frequency. The multiplexing factor can be raised with a large total readout bandwidth and small frequency spacing between channels. The inductance is kept constant to maintain a uniform readout bandwidth across detectors, while the maximum acceptable value is determined by bolometer stability. Current technology relies on commercially available ceramic chip capacitors. These have high scatter in their capacitance thereby requiring large frequency spacing. Furthermore, they have high equivalent series resistance (ESR) at higher frequencies and are time consuming and tedious to hand assemble via soldering. A solution lies in lithographed, planar spiral inductors (currently in use by some experiments) combined with interdigitated capacitors on a silicon (Si) substrate. To maintain reasonable device dimensions, we have reduced trace and gap widths of the LCs to 4 \\upmu m. We increased the inductance from 16 to 60 \\upmu H to achieve a higher packing density, a requirement for FDM systems with large multiplexing factors. Additionally, the Si substrate yields low ESR values across the entire frequency range and lithography makes mass production of LC pairs possible. We reduced mutual inductance between inductors by placing them in a checkerboard pattern with the capacitors, thereby increasing physical distances between adjacent inductors. We also reduce magnetic coupling of inductors with external sources by evaporating a superconducting ground plane onto the backside of the substrate. We report on the development of lithographed LCs in the 1-5 MHz range for use

  4. Microwave multiplex readout for superconducting sensors

    NASA Astrophysics Data System (ADS)

    Ferri, E.; Becker, D.; Bennett, D.; Faverzani, M.; Fowler, J.; Gard, J.; Giachero, A.; Hays-Wehle, J.; Hilton, G.; Maino, M.; Mates, J.; Puiu, A.; Nucciotti, A.; Reintsema, C.; Schmidt, D.; Swetz, D.; Ullom, J.; Vale, L.

    2016-07-01

    The absolute neutrino mass scale is still an outstanding challenge in both particle physics and cosmology. The calorimetric measurement of the energy released in a nuclear beta decay is a powerful tool to determine the effective electron-neutrino mass. In the last years, the progress on low temperature detector technologies has allowed to design large scale experiments aiming at pushing down the sensitivity on the neutrino mass below 1 eV. Even with outstanding performances in both energy (~ eV on keV) and time resolution (~ 1 μs) on the single channel, a large number of detectors working in parallel is required to reach a sub-eV sensitivity. Microwave frequency domain readout is the best available technique to readout large array of low temperature detectors, such as Transition Edge Sensors (TESs) or Microwave Kinetic Inductance Detectors (MKIDs). In this way a multiplex factor of the order of thousands can be reached, limited only by the bandwidth of the available commercial fast digitizers. This microwave multiplexing system will be used to readout the HOLMES detectors, an array of 1000 microcalorimeters based on TES sensors in which the 163Ho will be implanted. HOLMES is a new experiment for measuring the electron neutrino mass by means of the electron capture (EC) decay of 163Ho. We present here the microwave frequency multiplex which will be used in the HOLMES experiment and the microwave frequency multiplex used to readout the MKID detectors developed in Milan as well.

  5. Read-out optical schemes for holographic memory system based on multiplexed computer generated 1D Fourier holograms

    NASA Astrophysics Data System (ADS)

    Donchenko, Sergey S.; Odinokov, Sergey B.; Bobrinev, Vladimir I.; Betin, Alexandr Y.; Zlokazov, Evgenie Y.

    2015-05-01

    Computer holographic synthesis allows to significantly simplify the recording scheme of microholograms in holographic memory system as the classic high precision holographic setup based on two-beam interference is removed by simple scale reduction projection scheme. Application of computer generated 1D-Fourier holograms provides the possibility of selective reconstruction of the multiplexed holograms with different orientation of data lines by corresponding rotation of anamorphic objective (cylindrical lens), used in the read-out systems. Two configurations of read-out optical scheme were investigated by our team: full-page scheme and line-by-line scheme. In the present article we report the specificities of these schemes and consider their advantages and disadvantages. The results of experimental modeling of both read-out configurations are also presented.

  6. Delay grid multiplexing: simple time-based multiplexing and readout method for silicon photomultipliers

    NASA Astrophysics Data System (ADS)

    Won, Jun Yeon; Ko, Guen Bae; Lee, Jae Sung

    2016-10-01

    In this paper, we propose a fully time-based multiplexing and readout method that uses the principle of the global positioning system. Time-based multiplexing allows simplifying the multiplexing circuits where the only innate traces that connect the signal pins of the silicon photomultiplier (SiPM) channels to the readout channels are used as the multiplexing circuit. Every SiPM channel is connected to the delay grid that consists of the traces on a printed circuit board, and the inherent transit times from each SiPM channel to the readout channels encode the position information uniquely. Thus, the position of each SiPM can be identified using the time difference of arrival (TDOA) measurements. The proposed multiplexing can also allow simplification of the readout circuit using the time-to-digital converter (TDC) implemented in a field-programmable gate array (FPGA), where the time-over-threshold (ToT) is used to extract the energy information after multiplexing. In order to verify the proposed multiplexing method, we built a positron emission tomography (PET) detector that consisted of an array of 4  ×  4 LGSO crystals, each with a dimension of 3  ×  3  ×  20 mm3, and one- to-one coupled SiPM channels. We first employed the waveform sampler as an initial study, and then replaced the waveform sampler with an FPGA-TDC to further simplify the readout circuits. The 16 crystals were clearly resolved using only the time information obtained from the four readout channels. The coincidence resolving times (CRTs) were 382 and 406 ps FWHM when using the waveform sampler and the FPGA-TDC, respectively. The proposed simple multiplexing and readout methods can be useful for time-of-flight (TOF) PET scanners.

  7. TES Detector Noise Limited Readout Using SQUID Multiplexers

    NASA Technical Reports Server (NTRS)

    Staguhn, J. G.; Benford, D. J.; Chervenak, J. A.; Khan, S. A.; Moseley, S. H.; Shafer, R. A.; Deiker, S.; Grossman, E. N.; Hilton, G. C.; Irwin, K. D.

    2004-01-01

    The availability of superconducting Transition Edge Sensors (TES) with large numbers of individual detector pixels requires multiplexers for efficient readout. The use of multiplexers reduces the number of wires needed between the cryogenic electronics and the room temperature electronics and cuts the number of required cryogenic amplifiers. We are using an 8 channel SQUID multiplexer to read out one-dimensional TES arrays which are used for submillimeter astronomical observations. We present results from test measurements which show that the low noise level of the SQUID multiplexers allows accurate measurements of the TES Johnson noise, and that in operation, the readout noise is dominated by the detector noise. Multiplexers for large number of channels require a large bandwidth for the multiplexed readout signal. We discuss the resulting implications for the noise performance of these multiplexers which will be used for the readout of two dimensional TES arrays in next generation instruments.

  8. Demonstration of Time Domain Multiplexed Readout for Magnetically Coupled Calorimeters

    NASA Technical Reports Server (NTRS)

    Porst, J.-P.; Adams, J. S.; Balvin, M.; Bandler, S.; Beyer, J.; Busch, S. E.; Drung, D.; Seidel, G. M.; Smith, S. J.; Stevenson, T. R.

    2012-01-01

    Magnetically coupled calorimeters (MCC) have extremely high potential for x-ray applications due to the inherent high energy resolution capability and being non-dissipative. Although very high energy-resolution has been demonstrated, until now there has been no demonstration of multiplexed read-out. We report on the first realization of a time domain multiplexed (TDM) read-out. While this has many similarities with TDM of transition-edge-sensors (TES), for MGGs the energy resolution is limited by the SQUID read-out noise and requires the well established scheme to be altered in order to minimize degradation due to noise aliasing effects. In cur approach, each pixel is read out by a single first stage SQUID (SQ1) that is operated in open loop. The outputs of the SQ1 s are low-pass filtered with an array of low cross-talk inductors, then fed into a single-stage SQUID TD multiplexer. The multiplexer is addressed from room temperature and read out through a single amplifier channel. We present results achieved with a new detector platform. Noise performance is presented and compared to expectations. We have demonstrated multiplexed X-ray spectroscopy at 5.9keV with delta_FWHM=10eV. In an optimized setup, we show it is possible to multiplex 32 detectors without significantly degrading the Intrinsic detector resolution.

  9. Digital frequency domain multiplexing readout electronics for the next generation of millimeter telescopes

    NASA Astrophysics Data System (ADS)

    Bender, Amy N.; Cliche, Jean-François; de Haan, Tijmen; Dobbs, Matt A.; Gilbert, Adam J.; Montgomery, Joshua; Rowlands, Neil; Smecher, Graeme M.; Smith, Ken; Wilson, Andrew

    2014-07-01

    Frequency domain multiplexing (fMux) is an established technique for the readout of transition-edge sensor (TES) bolometers in millimeter-wavelength astrophysical instrumentation. In fMux, the signals from multiple detectors are read out on a single pair of wires reducing the total cryogenic thermal loading as well as the cold component complexity and cost of a system. The current digital fMux system, in use by POLARBEAR, EBEX, and the South Pole Telescope, is limited to a multiplexing factor of 16 by the dynamic range of the Superconducting Quantum Interference Device pre-amplifier and the total system bandwidth. Increased multiplexing is key for the next generation of large format TES cameras, such as SPT-3G and POLARBEAR2, which plan to have on the of order 15,000 detectors. Here, we present the next generation fMux readout, focusing on the warm electronics. In this system, the multiplexing factor increases to 64 channels per module (2 wires) while maintaining low noise levels and detector stability. This is achieved by increasing the system bandwidth, reducing the dynamic range requirements though active feedback, and digital synthesis of voltage biases with a novel polyphase filter algorithm. In addition, a version of the new fMux readout includes features such as low power consumption and radiation-hard components making it viable for future space-based millimeter telescopes such as the LiteBIRD satellite.

  10. Multiplexed Readout of Thermal Bolometers with Superconducting Transition Edge Thermometers

    NASA Technical Reports Server (NTRS)

    Benford, Dominic J.; Allen, Christine A.; Chervenak, James A.; Freund, Mino M.; Kutyrev, Alexander S.; Moseley, S. Harvey; Shafer, Richard A.; Staguhn, Johannes G.; Grossman, Erich N.; Hilton, Gene C.

    2001-01-01

    History shows that in astronomy, more is better. In the near future, direct detector arrays for the far-infrared and submillimeter will contain hundreds to thousands of elements. A multiplexed readout is necessary for practical implementation of such arrays, and has been developed using SQUIDs. The technology permits a 32 x 32 array of bolometers to be read out using approximately 100 wires rather than the >2000 needed with direct wiring. These bolometer arrays are made by micromachining techniques, using superconducting transition edge sensors as the thermistors. We describe the development of this multiplexed superconducting bolometer array architecture as a step toward bringing about the first astronomically useful arrays of this design. This technology will be used in the Submillimeter and Far Infrared Experiment (SAFIRE) instrument on Stratospheric Observatory for Infrared Astronomy (SOFIA), and is a candidate for a wide variety of other spectroscopic and photometric instruments.

  11. A multiplex readout method for position sensitive boron coated straw neutron detector

    NASA Astrophysics Data System (ADS)

    Yu, Hao; Gong, Hui; Li, Jianmin; Wang, Yongqiang; Wang, Xuewu; Li, Yuanjing; Kang, Kejun

    2015-10-01

    A 1 m×1 m boron coated straw neutron detector is expected to be used to build the small-angle neutron scattering (SANS) instrument of the Compact Pulsed Hadron Source (CPHS) in Tsinghua University. A multiplex readout method based on summing circuits in columns and rows is studied for this large area position sensitive detector. In this method, the outputs of charge sensitive preamplifiers are combined by columns and rows at two ends of the detector, and then the shaped signals are sampled by flash ADCs. With the position reconstructed algorithm implemented in FPGA which analyzes the charge division and column and row number of signals, the 3-D position information of neutron events can be obtained. The position resolution and counting rate performance of this method are analyzed, and the comparison to the delay-line readout method is also given. With the multiplex readout method, the scale of readout electronics can be greatly reduced and a good position resolution can be reached. A readout electronics system for a detector module which consists 4 × 10 straw tubes is designed based on this method, and the test with neutron beam shows an average 3-D spatial resolution of 4 × 4 × 6.8mm3.

  12. A balanced, superconducting multiplier circuit for fast-switching and multiplexed qubit readout: Performance and demonstration

    NASA Astrophysics Data System (ADS)

    Moores, Brad A.; Chapman, Benjamin J.; Rosenthal, Eric I.; Kerckhoff, Joseph; Lehnert, K. W.

    A major challenge of scaling the promising transmon qubits into a quantum information processing machine is the classical hardware burden required to readout many qubits. Within the cavity QED architecture, qubit states are measured by detecting the transmission through microwave cavities. A multiplexing scheme could allow the classical hardware burden of generating and measuring a readout tone to be shared among several cavity-qubit systems. In this talk, we will present measurements of a recently designed superconducting multiplier circuit intended to accomplish time and code domain multiplexed readout. In particular, we characterize three modes of microwave operation: transmission, reflection and inversion. The device can be switched between these modes approximately 100 times faster than typical qubit coherence times. Exploiting this performance, we demonstrate a code domain multiplexing scheme with classical signals created to simulate typical qubit signals. The scheme operates with near unity fidelity at microwave powers comparable to typical qubit tones. This work is supported by the ARO under the Contract W911NF-14-1-0079.

  13. Medipix2 parallel readout system

    NASA Astrophysics Data System (ADS)

    Fanti, V.; Marzeddu, R.; Randaccio, P.

    2003-08-01

    A fast parallel readout system based on a PCI board has been developed in the framework of the Medipix collaboration. The readout electronics consists of two boards: the motherboard directly interfacing the Medipix2 chip, and the PCI board with digital I/O ports 32 bits wide. The device driver and readout software have been developed at low level in Assembler to allow fast data transfer and image reconstruction. The parallel readout permits a transfer rate up to 64 Mbytes/s. http://medipix.web.cern ch/MEDIPIX/

  14. A near-infrared 64-pixel superconducting nanowire single photon detector array with integrated multiplexed readout

    SciTech Connect

    Allman, M. S. Verma, V. B.; Stevens, M.; Gerrits, T.; Horansky, R. D.; Lita, A. E.; Mirin, R.; Nam, S. W.; Marsili, F.; Beyer, A.; Shaw, M. D.; Kumor, D.

    2015-05-11

    We demonstrate a 64-pixel free-space-coupled array of superconducting nanowire single photon detectors optimized for high detection efficiency in the near-infrared range. An integrated, readily scalable, multiplexed readout scheme is employed to reduce the number of readout lines to 16. The cryogenic, optical, and electronic packaging to read out the array as well as characterization measurements are discussed.

  15. Hodoscope readout system

    DOEpatents

    Lee, L.Y.

    1973-12-01

    A readout system has been provided for reading out a radiation multidetector device with a reduced number of signal sensors. A radiation hodoscope, such as an array of scintillation counters, multiwire proportional counter array, or a set of multidetectors which do not receive signals simultaneously, is divided into equal numbered groups. A first group of signal terminals is connected to the equal numbered groups of detectors so that a signal from any one of the detectors of a group will be fed to one of the first group of terminals. A second group of signal terminals is connected to the detector groups so that a signal from a particular numbered detector of each of the detector groups is connected to one of the second group of terminals. Both groups of signal terminals are, in turn, coupled to signal sensors so that when a signal is simultaneously observed in one of the first group of terminals and one of the second group of tenniinals the specific detector detecting a radiation event is determined. The sensors are arranged in such a manner that a binary code is developed from their outputs which can be stored in a digital storage means according to the location of the event in the multidetector device. (Official Gazette)

  16. Multiplex television transmission system

    NASA Technical Reports Server (NTRS)

    Reed, W. R.

    1967-01-01

    Time-multiplexing system enables several cameras to share a single commercial television transmission channel. This system is useful in industries for visually monitoring several operating areas or instrument panels from a remote location.

  17. Code-division-multiplexed readout of large arrays of TES microcalorimeters

    NASA Astrophysics Data System (ADS)

    Morgan, K. M.; Alpert, B. K.; Bennett, D. A.; Denison, E. V.; Doriese, W. B.; Fowler, J. W.; Gard, J. D.; Hilton, G. C.; Irwin, K. D.; Joe, Y. I.; O'Neil, G. C.; Reintsema, C. D.; Schmidt, D. R.; Ullom, J. N.; Swetz, D. S.

    2016-09-01

    Code-division multiplexing (CDM) offers a path to reading out large arrays of transition edge sensor (TES) X-ray microcalorimeters with excellent energy and timing resolution. We demonstrate the readout of X-ray TESs with a 32-channel flux-summed code-division multiplexing circuit based on superconducting quantum interference device (SQUID) amplifiers. The best detector has energy resolution of 2.28 ± 0.12 eV FWHM at 5.9 keV and the array has mean energy resolution of 2.77 ± 0.02 eV over 30 working sensors. The readout channels are sampled sequentially at 160 ns/row, for an effective sampling rate of 5.12 μs/channel. The SQUID amplifiers have a measured flux noise of 0.17 μΦ0/√Hz (non-multiplexed, referred to the first stage SQUID). The multiplexed noise level and signal slew rate are sufficient to allow readout of more than 40 pixels per column, making CDM compatible with requirements outlined for future space missions. Additionally, because the modulated data from the 32 SQUID readout channels provide information on each X-ray event at the row rate, our CDM architecture allows determination of the arrival time of an X-ray event to within 275 ns FWHM with potential benefits in experiments that require detection of near-coincident events.

  18. Multiplexing Readout of TES Microcalorimeters Based on Analog Baseband Feedback

    SciTech Connect

    Takei, Y.; Yamasaki, N.Y; Mitsuda, K.; Kimura, S.; Hirakoso, W.; Masui, K.; Korte, P. A. J. de; Kuur, J. van der; Gottardi, L.

    2009-12-16

    A TES microcalorimeter array is a promising spectrometer with excellent energy resolution and a moderate imaging capability. To realize a large format array in space, multiplexing the TES signals at the low tempersture stage is mandatory. We are developing frequency division multiplexing (FDM) based on baseband feedback technique. In FDM, each TES is AC-biased with a different carrier frequency. Signals from several pixels are summed and then read out by one SQUID. The maximum number of multiplexed pixels are limited by the frequency band in which the SQUID can be operated in a flux-locked loop, which is {approx}1 MHz with standard flux-locked loop circuit. In the baseband feedback, the signal ({approx}10 kHz band) from the TES is once demodulated. Then a reconstructed copy of the modulated signal with an appropriate phase is fed back to the SQUID input coil to maintain an approximately constant magnetic flux. This can be implemented even for large cable delays and automatically suppresses the carrier. We developed a prototype electronics for the baseband feedback based on an analog phase sensitive detector (PSD) and a multiplier. Combined with Seiko 80-SSA SQUID amp, open-loop gain of 8 has been obtained for 10 kHz baseband signal at 5 MHz carrier frequency, with a moderate noise contribution of 27pA/{radical}(Hz) at input.

  19. Indium Hybridization of Large Format TES Bolometer Arrays to Readout Multiplexers for Far-Infrared Astronomy

    NASA Technical Reports Server (NTRS)

    Miller, Timothy M.; Costen, Nick; Allen, Christine

    2007-01-01

    This conference poster reviews the Indium hybridization of the large format TES bolometer arrays. We are developing a key technology to enable the next generation of detectors. That is the Hybridization of Large Format Arrays using Indium bonded detector arrays containing 32x40 elements which conforms to the NIST multiplexer readout architecture of 1135 micron pitch. We have fabricated and hybridized mechanical models with the detector chips bonded after being fully back-etched. The mechanical support consists of 30 micron walls between elements Demonstrated electrical continuity for each element. The goal is to hybridize fully functional array of TES detectors to NIST readout.

  20. Multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, E.S.; Li, Q.; Lu, X.

    1998-04-21

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

  1. Multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, E.S.; Chang, H.T.; Fung, E.N.; Li, Q.; Lu, X.

    1996-12-10

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

  2. Multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, Edward S.; Li, Qingbo; Lu, Xiandan

    1998-04-21

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

  3. Multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, Edward S.; Chang, Huan-Tsang; Fung, Eliza N.; Li, Qingbo; Lu, Xiandan

    1996-12-10

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

  4. Chopped molecular beam multiplexing system

    NASA Technical Reports Server (NTRS)

    Adams, Billy R. (Inventor)

    1986-01-01

    The integration of a chopped molecular beam mass spectrometer with a time multiplexing system is described. The chopping of the molecular beam is synchronized with the time intervals by a phase detector and a synchronous motor. Arithmetic means are generated for phase shifting the chopper with respect to the multiplexer. A four channel amplifier provides the capacity to independently vary the baseline and amplitude in each channel of the multiplexing system.

  5. Semiconductor arrays with multiplexer readout for gamma-ray imaging: results for a 48 × 48 Ge array

    NASA Astrophysics Data System (ADS)

    Barber, H. B.; Augustine, F. L.; Barrett, H. H.; Dereniak, E. L.; Matherson, K. L.; Meyers, T. J.; Perry, D. L.; Venzon, J. E.; Woolfenden, J. M.; Young, E. T.

    1994-12-01

    We are developing a new kind of gamma-ray imaging device that has sub-millimeter spatial resolution and excellent energy resolution. The device is composed of a slab of semiconductor detector partitioned into an array of detector cells by photolithography and connected to a monolithic circuit chip called a multiplexer (MUX) for readout. Our application is for an ultra-high-resolution SPECT system for functional brain imaging using an injected radiotracer. We report here on results obtained with a Hughes 48 × 48 Ge PIN-photodiode array with MUX readout, originally developed as an infrared focal-plane-array imaging sensor. The device functions as an array of individual gamma-ray detectors with minimal interpixel crosstalk. Linearity of energy response is excellent up to at least 140 keV. The array exhibits excellent energy resolution, ˜ 2 keV at ≤ 140 keV or 1.5% FWHM at 140 keV. The energy resolution is dominated by MUX readout noise and so should improve with MUX optimization for gamma-ray detection. The spatial resolution of the 48 × 48 Ge array is essentially the same as the pixel spacing, 125 μm. The quantum efficiency is limited by the thin Ge detector (0.25 mm), but this approach is readily applicable to thicker Ge detectors and room-temperature semiconductor detectors such as CdTe, HgI 2 and CdZnTe.

  6. General purpose multiplexing device for cryogenic microwave systems

    NASA Astrophysics Data System (ADS)

    Chapman, Benjamin J.; Moores, Bradley A.; Rosenthal, Eric I.; Kerckhoff, Joseph; Lehnert, K. W.

    2016-05-01

    We introduce and experimentally characterize a general purpose device for signal processing in circuit quantum electrodynamics systems. The device is a broadband two-port microwave circuit element with three modes of operation: it can transmit, reflect, or invert incident signals between 4 and 8 GHz. This property makes it a versatile tool for lossless signal processing at cryogenic temperatures. In particular, rapid switching (≤ 15 ns ) between these operation modes enables several multiplexing readout protocols for superconducting qubits. We report the device's performance in a two-channel code domain multiplexing demonstration. The multiplexed data are recovered with fast readout times (up to 400 ns ) and infidelities ≤ 10-2 for probe powers ≥ 7 fW , in agreement with the expectation for binary signaling with Gaussian noise.

  7. Multiplexed FBG strain measurement system

    NASA Astrophysics Data System (ADS)

    Helsztynski, Jerzy; Lewandowski, Lech; Jasiewicz, Wieslaw; Jedrzejewski, Kazimierz P.

    2008-11-01

    The idea and, design and realization of fiber Bragg grating multiplexing system is given. Special Bragg gratings with very long and linear side slopes were practically realized. They were tuned for different wavelengths distanced 9nm in each measurement channel. The system was applied for strain control. Special spectrophotometer with linear photodiode array was made. For flexible distributed multiplexing of many sensor channels fiber-optic switches were used.

  8. Indium Hybridization of Large Format TES Bolometer Arrays to Readout Multiplexers for Far-Infrared Astronomy

    NASA Technical Reports Server (NTRS)

    Miller, Timothy M.; Costen, Nick; Allen, Christine

    2007-01-01

    The advance of new detector technologies combined with enhanced fabrication methods has resulted in an increase in development of large format arrays. The next generation of scientific instruments will utilize detectors containing hundreds to thousands of elements providing a more efficient means to conduct large area sky surveys. Some notable detectors include a 32x32 x-ray microcalorimeter for Constellation-X, an infrared bolometer called SAFIRE to fly on the airborne observatory SOFIA, and the sub-millimeter bolometer SCUBA-2 to be deployed at the JCMT which will use more than 10,000 elements for two colors, each color using four 32x40 arrays. Of these detectors, SCUBA-2 is farthest along in development and uses indium hybridization to multiplexers for readout of the large number of elements, a technology that will be required to enable the next generation of large format arrays. Our current efforts in working toward large format arrays have produced GISMO, the Goddard IRAM Superconducting 2-Millimeter observer. GISMO is a far infrared instrument to be field tested later this year at the IRAM 30 meter telescope in Spain. GISMO utilizes transition edge sensor (TES) technology in an 8x16 filled array format that allows for typical fan-out wiring and wire-bonding to four 1x32 NIST multiplexers. GISMO'S electrical wiring is routed along the tops of 30 micron walls which also serve as the mechanical framework for the array. This architecture works well for the 128 element array, but is approaching the limit for routing the necessary wires along the surface while maintaining a high fill factor. Larger format arrays will benefit greatly from making electrical connections through the wafer to the backside, where they can be hybridized to a read-out substrate tailored to handling the wiring scheme. The next generation array we are developing is a 32x40 element array on a pitch of 1135 microns that conforms to the NIST multiplexer, already developed for the SCUBA-2

  9. Measurement of MKID Performance with High-Speed and Wide-Band Readout System

    NASA Astrophysics Data System (ADS)

    Karatsu, Kenichi; Naruse, M.; Nitta, T.; Sekine, M.; Sekiguchi, S.; Sekimoto, Y.; Noguchi, T.; Uzawa, Y.; Matsuo, H.; Kiuchi, H.

    2014-08-01

    Microwave kinetic inductance detectors (MKIDs) are being developed at the National Astronomical Observatory of Japan to enable precise measurements of the cosmic microwave background. One of the features of MKIDs is scalability using a frequency-division multiplexing (FDMUX) readout scheme. A digital fast fourier transform spectrometer (FFTS) is a good way to read out a number of resonance frequencies simultaneously and fully utilize the advantage of FDMUX of MKIDs. We have developed FFTS readout electronics using an ADC/DAC with 1 Gsps (sample per second) sampling rate and 270 MHz bandwidth. We measured the noise characteristics of a single MKID in the frequency range of 60 Hz-30 kHz with this readout system, and found the noise was almost equivalent to the noise measured by ordinary analog IQ down-converter readout. This indicates our FFTS electronics do not add any additional noise to the MKID readout system over the frequency range.

  10. Integrated multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, Edward S.; Tan, Hongdong

    2002-05-14

    The present invention provides an integrated multiplexed capillary electrophoresis system for the analysis of sample analytes. The system integrates and automates multiple components, such as chromatographic columns and separation capillaries, and further provides a detector for the detection of analytes eluting from the separation capillaries. The system employs multiplexed freeze/thaw valves to manage fluid flow and sample movement. The system is computer controlled and is capable of processing samples through reaction, purification, denaturation, pre-concentration, injection, separation and detection in parallel fashion. Methods employing the system of the invention are also provided.

  11. Indium Hybridization of Large Format TES Bolometer Arrays to Readout Multiplexers for Far-Infrared Astronomy

    NASA Astrophysics Data System (ADS)

    Miller, Timothy M.; Costen, Nick; Allen, Christine

    2008-04-01

    In our effort to develop large format kilo-pixel arrays of transition edge sensors, we are working toward demonstrating electrical connectivity of a hybridized 32×40 element detector to a NIST multiplexer. The geometry of large-scale arrays that attempt to maintain a high filling factor does not allow for typical fan-out wiring, thus the detector array is hybridized directly to the readout multiplexer. The two parts are bonded together with 10 micron tall indium bumps, which complete the electrical path and also provide mechanical strength. The indium hybridization was developed and performed at the Goddard Space Flight Center and has been shown to withstand tensile loading up to 0.26 gram-force per indium bump. A cavity is deep etched behind each pixel prior to bonding, leaving only 30 micron support walls, which maintains a high filling fraction. Electrical continuity has been shown through the indium path in room temperature measurements. We report on the fabrication process used to hybridize the parts and the subsequent electrical performance.

  12. White noise of Nb-based microwave superconducting quantum interference device multiplexers with NbN coplanar resonators for readout of transition edge sensors

    SciTech Connect

    Kohjiro, Satoshi; Hirayama, Fuminori; Yamamori, Hirotake; Nagasawa, Shuichi; Fukuda, Daiji; Hidaka, Mutsuo

    2014-06-14

    White noise of dissipationless microwave radio frequency superconducting quantum interference device (RF-SQUID) multiplexers has been experimentally studied to evaluate their readout performance for transition edge sensor (TES) photon counters ranging from near infrared to gamma ray. The characterization has been carried out at 4 K, first to avoid the low-frequency fluctuations present at around 0.1 K, and second, for a feasibility study of readout operation at 4 K for extended applications. To increase the resonant Q at 4 K and maintain low noise SQUID operation, multiplexer chips consisting of niobium nitride (NbN)-based coplanar-waveguide resonators and niobium (Nb)-based RF-SQUIDs have been developed. This hybrid multiplexer exhibited 1 × 10{sup 4} ≤ Q ≤ 2 × 10{sup 4} and the square root of spectral density of current noise referred to the SQUID input √S{sub I} = 31 pA/√Hz. The former and the latter are factor-of-five and seven improvements from our previous results on Nb-based resonators, respectively. Two-directional readout on the complex plane of the transmission component of scattering matrix S{sub 21} enables us to distinguish the flux noise from noise originating from other sources, such as the cryogenic high electron mobility transistor (HEMT) amplifier. Systematic noise measurements with various microwave readout powers P{sub MR} make it possible to distinguish the contribution of noise sources within the system as follows: (1) The achieved √S{sub I} is dominated by the Nyquist noise from a resistor at 4 K in parallel to the SQUID input coil which is present to prevent microwave leakage to the TES. (2) The next dominant source is either the HEMT-amplifier noise (for small values of P{sub MR}) or the quantization noise due to the resolution of 300-K electronics (for large values of P{sub MR}). By a decrease of these noise levels to a degree that is achievable by current technology, we predict that the

  13. White noise of Nb-based microwave superconducting quantum interference device multiplexers with NbN coplanar resonators for readout of transition edge sensors

    NASA Astrophysics Data System (ADS)

    Kohjiro, Satoshi; Hirayama, Fuminori; Yamamori, Hirotake; Nagasawa, Shuichi; Fukuda, Daiji; Hidaka, Mutsuo

    2014-06-01

    White noise of dissipationless microwave radio frequency superconducting quantum interference device (RF-SQUID) multiplexers has been experimentally studied to evaluate their readout performance for transition edge sensor (TES) photon counters ranging from near infrared to gamma ray. The characterization has been carried out at 4 K, first to avoid the low-frequency fluctuations present at around 0.1 K, and second, for a feasibility study of readout operation at 4 K for extended applications. To increase the resonant Q at 4 K and maintain low noise SQUID operation, multiplexer chips consisting of niobium nitride (NbN)-based coplanar-waveguide resonators and niobium (Nb)-based RF-SQUIDs have been developed. This hybrid multiplexer exhibited 1 × 104 ≤ Q ≤ 2 × 104 and the square root of spectral density of current noise referred to the SQUID input √SI = 31 pA/√Hz. The former and the latter are factor-of-five and seven improvements from our previous results on Nb-based resonators, respectively. Two-directional readout on the complex plane of the transmission component of scattering matrix S21 enables us to distinguish the flux noise from noise originating from other sources, such as the cryogenic high electron mobility transistor (HEMT) amplifier. Systematic noise measurements with various microwave readout powers PMR make it possible to distinguish the contribution of noise sources within the system as follows: (1) The achieved √SI is dominated by the Nyquist noise from a resistor at 4 K in parallel to the SQUID input coil which is present to prevent microwave leakage to the TES. (2) The next dominant source is either the HEMT-amplifier noise (for small values of PMR) or the quantization noise due to the resolution of 300-K electronics (for large values of PMR). By a decrease of these noise levels to a degree that is achievable by current technology, we predict that the microwave RF-SQUID multiplexer can exhibit √SI ≤ 5 pA/√Hz, i.e., close to √SI of

  14. A 48x48 CdZnTe array with multiplexer readout

    SciTech Connect

    Marks, D.G.; Barber, H.B.; Barrett, H.H.

    1996-06-01

    The authors report results of gamma-ray imaging and energy-resolution tests of a 48x48 CdZnTe array. The detectors have 125 {micro}m square pixel electrodes produced by photolithography and are indium-bump-bonded to a multiplexer readout circuit. Using a collimated beam of 140 keV gamma rays of 120 {micro}m diameter centered on one pixel, they found that the majority of events produced significant charge deposition in nearby pixels. Charge and energy are transported out of the pixel by charge diffusion, photoelectron range, Compton scattering, and escape of K x-rays. These effects also distort single-pixel spectra, although photopeaks are still discernible at 140 keV. When signals from neighboring pixels are summed together to correct for this charge spreading, an energy resolution of 10 keV is obtained at 140 keV. Corrections will be simpler and energy resolution should be better for the 380 {micro}m pixels of the 64x64 CdZnTe arrays the authors are constructing for an ultra-high-resolution brain imager.

  15. Embedded controller for GEM detector readout system

    NASA Astrophysics Data System (ADS)

    Zabołotny, Wojciech M.; Byszuk, Adrian; Chernyshova, Maryna; Cieszewski, Radosław; Czarski, Tomasz; Dominik, Wojciech; Jakubowska, Katarzyna L.; Kasprowicz, Grzegorz; Poźniak, Krzysztof; Rzadkiewicz, Jacek; Scholz, Marek

    2013-10-01

    This paper describes the embedded controller used for the multichannel readout system for the GEM detector. The controller is based on the embedded Mini ITX mainboard, running the GNU/Linux operating system. The controller offers two interfaces to communicate with the FPGA based readout system. FPGA configuration and diagnostics is controlled via low speed USB based interface, while high-speed setup of the readout parameters and reception of the measured data is handled by the PCI Express (PCIe) interface. Hardware access is synchronized by the dedicated server written in C. Multiple clients may connect to this server via TCP/IP network, and different priority is assigned to individual clients. Specialized protocols have been implemented both for low level access on register level and for high level access with transfer of structured data with "msgpack" protocol. High level functionalities have been split between multiple TCP/IP servers for parallel operation. Status of the system may be checked, and basic maintenance may be performed via web interface, while the expert access is possible via SSH server. System was designed with reliability and flexibility in mind.

  16. Study of spacecraft direct readout meteorological systems

    NASA Technical Reports Server (NTRS)

    Bartlett, R.; Elam, W.; Hoedemaker, R.

    1973-01-01

    Characteristics are defined of the next generation direct readout meteorological satellite system with particular application to Tiros N. Both space and ground systems are included. The recommended space system is composed of four geosynchronous satellites and two low altitude satellites in sun-synchronous orbit. The goesynchronous satellites transmit to direct readout ground stations via a shared S-band link, relayed FOFAX satellite cloud cover pictures (visible and infrared) and weather charts (WEFAX). Basic sensor data is transmitted to regional Data Utilization Stations via the same S-band link. Basic sensor data consists of 0.5 n.m. sub-point resolution data in the 0.55 - 0.7 micron spectral region, and 4.0 n.m. resolution data in the 10.5 - 12.6 micron spectral region. The two low altitude satellites in sun-synchronous orbit provide data to direct readout ground stations via a 137 MHz link, a 400 Mhz link, and an S-band link.

  17. Laser multiplexing system

    DOEpatents

    Johnson, Steve A.; English, Jr., Ronald Edward; White, Ronald K.

    2001-01-01

    A plurality of copper lasers, as radiant power sources, emits a beam of power carrying radiation. A plurality of fiber injection assemblies receives power from the plurality of copper lasers and injects such power into a plurality of fibers for individually transmitting the received power to a plurality of power-receiving devices. The power-transmitting fibers of the system are so arranged that power is delivered therethrough to each of the power-receiving devices such that, even if a few of the radiant power sources and/or fibers fail, the power supply to any of the power receiving devices will not completely drop to zero but will drop by the same proportionate amount.

  18. Architecture of a modular, multichannel readout system for dense electrochemical biosensor microarrays

    NASA Astrophysics Data System (ADS)

    Ramfos, Ioannis; Blionas, Spyridon; Birbas, Alexios

    2015-01-01

    The architecture of a modular, multichannel readout system for dense electrochemical microarrays, targeting Lab-on-a-Chip applications, is presented. This approach promotes efficient component reusability through a hybrid multiplexing methodology, maintaining high levels of sampling performance and accuracy. Two readout modes are offered, which can be dynamically interchanged following signal profiling, to cater for both rapid signal transitions and weak current responses. Additionally, functional extensions to the described architecture are discussed, which provide the system with multi-biasing capabilities. A prototype integrated circuit of the proposed architecture’s analog core and a supporting board were implemented to verify the working principles. The system was evaluated using standard loads, as well as electrochemical sensor arrays. Through a range of operating conditions and loads, the prototype exhibited a highly linear response and accurately delivered the readout of input signals with fast transitions and wide dynamic ranges.

  19. Asynchronous data readout system for multichannel ASIC

    NASA Astrophysics Data System (ADS)

    Ivanov, P. Y.; Atkin, E. V.

    2016-02-01

    The data readout system of multichannel data-driven ASIC, requiring high-speed (320 Mb/s) output data serialization is described. Its structure, based on a limited number of FIFO blocks, provides a lossless data transfer. The solution has been realized as a separate test IP block in the prototyped 8 channel ASIC, intended for the muon chamber of CBM experiment at FAIR. The block was developed for the UMC 0.18 μm MMRF CMOS process and prototyped via Europractice. Main parameters of the chip are given.

  20. Integrated multi-crate FERA readout system

    SciTech Connect

    Kistryn, S.; Bee, C.P.; Eberhardt, P.

    1997-12-31

    We discuss a moderate-size readout system based entirely on FERA compatible units. The implementation of a specially developed FERA Extender module is presented, whose main feature is the ability to distribute the system over many CAMAC crates. This provides a convenient way of splitting the FERA bus into several virtually independent sub-systems driven by individual gate signals. Tagging of the event fragments from each sub-system with an event number incremented on the arrival of each master gate, provides a convenient means of reconstructing the full event at a later stage. An example of the external supplementary FERA control logic required for a complex multi-crate and multi-gate system controlled by a single FERA Manager, is also discussed together with some remarks on the system performance.

  1. Multiplexed optical operation of distributed nanoelectromechanical systems arrays.

    PubMed

    Sampathkumar, A; Ekinci, K L; Murray, T W

    2011-03-01

    We report a versatile all optical technique to excite and read-out a distributed nanoelectromechanical systems (NEMS) array. The NEMS array is driven by a distributed, intensity modulated optical pump through the photothermal effect. The ensuing vibrational response of the array is multiplexed onto a single probe beam in the form of a high frequency phase modulation. The phase modulation is optically down converted to a low frequency intensity modulation using an adaptive full-field interferometer, and subsequently detected using a CCD array. Rapid and single step mechanical characterization of ∼44 nominally identical high-frequency resonators is demonstrated. The technique may enable sensitivity improvements over single NEMS resonators by averaging signals coming from a multitude of devices in the array. In addition, the diffraction limited spatial resolution may allow for position-dependent read-out of NEMS sensor chips for sensing multiple analytes or spatially inhomogeneous forces. PMID:21287999

  2. A new interpolation arithmetic based readout signals process method for infrared imaging system applications

    NASA Astrophysics Data System (ADS)

    Li, Xinyi; Yao, Suying; Zhao, Yiqiang

    2009-07-01

    A new readout signals process circuit for infrared focal plane array (IR FPA) applications is proposed. In the proposed structure the continuous-time current signals from the detector array are mirrored, amplified, integrated on the integration capacitors and changed to discrete analog voltage signals. Next, these voltage signals are amplified and modulated by a group of encoded signals from the column buses, then fed to a multiple-input analog adder to generate a single serial output data stream. The generated single serial data stream is transferred to the mitigate noise circuit and is converted to digital signals by the A/D converter. For very large format detector arrays applications the speed restriction of the time-multiplexing circuitry and the A/D converter will be released. Since no scan technique has been used, all the output signals from an entire row in the detector array have been readout simultaneously without loss of optical power, the scalability of the photon-signals, the readout efficiency and the accuracy of the imaging system will be improved. Theory analysis and experimental results show that the proposed idea is reasonable and efficient. The proposed readout method is a solid option for large format infrared detector arrays and highly integrated infrared imaging system applications. In addition, the proposed idea also can be used for other active and passive imaging readout integrated circuits.

  3. Optical Multiplex Systems For Vehicles

    NASA Astrophysics Data System (ADS)

    Rogers, Wesley A.

    1980-09-01

    Optical multiplex technology, presently in vogue in many segments of industry, is now under scrutiny at General Motors. We are evaluating this technology as a means of simplifying the vehicle harness, reducing weight, eliminating electromagnetic interference, and providing drastically new interior styling options. Door, seat, steering column, forward and rear harness vehicle assemblies, are replaced with a single fiber optic cable in each area. A four bit microprocessor at the top of the steering column, and in each door, multiplexes control console button commands over fiber optic cables. A microprocessor at the other end of the cables decodes the optical signals and operates seats, windows, windshield wipers, etc.

  4. Self-triggering readout system for the neutron lifetime experiment PENeLOPE

    NASA Astrophysics Data System (ADS)

    Gaisbauer, D.; Konorov, I.; Steffen, D.; Paul, S.

    2016-07-01

    The aim of PENeLOPE (Precision Experiment on Neutron Lifetime Operating with Proton Extraction) at the Forschungsreaktor München II is a high-precision measurement of the neutron lifetime and thereby an improvement of the parameter's precision by one order of magnitude. In order to achieve a higher accuracy, modern experiments naturally require state-of-the-art readout electronics, as well as high-performance data acquisition systems. This paper presents the self-triggering readout system designed for PENeLOPE which features a continuous pedestal tracking, configurable signal detection logic, floating ground up to 30 kV, cryogenic environment and the novel Switched Enabling Protocol (SEP). The SEP is a time-division multiplexing transport level protocol developed for a star network topology.

  5. Multiplexed Oversampling Digitizer in 65 nm CMOS for Column-Parallel CCD Readout

    SciTech Connect

    Grace, Carl; Walder, Jean-Pierre; von der Lippe, Henrik

    2012-04-10

    A digitizer designed to read out column-parallel charge-coupled devices (CCDs) used for high-speed X-ray imaging is presented. The digitizer is included as part of the High-Speed Image Preprocessor with Oversampling (HIPPO) integrated circuit. The digitizer module comprises a multiplexed, oversampling, 12-bit, 80 MS/s pipelined Analog-to-Digital Converter (ADC) and a bank of four fast-settling sample-and-hold amplifiers to instrument four analog channels. The ADC multiplexes and oversamples to reduce its area to allow integration that is pitch-matched to the columns of the CCD. Novel design techniques are used to enable oversampling and multiplexing with a reduced power penalty. The ADC exhibits 188 ?V-rms noise which is less than 1 LSB at a 12-bit level. The prototype is implemented in a commercially available 65 nm CMOS process. The digitizer will lead to a proof-of-principle 2D 10 Gigapixel/s X-ray detector.

  6. A double photomultiplier Compton camera and its readout system for mice imaging

    NASA Astrophysics Data System (ADS)

    Fontana, Cristiano Lino; Atroshchenko, Kostiantyn; Baldazzi, Giuseppe; Bello, Michele; Uzunov, Nikolay; Di Domenico, Giovanni Di

    2013-04-01

    We have designed a Compton Camera (CC) to image the bio-distribution of gamma-emitting radiopharmaceuticals in mice. A CC employs the "electronic collimation", i.e. a technique that traces the gamma-rays instead of selecting them with physical lead or tungsten collimators. To perform such a task, a CC measures the parameters of the Compton interaction that occurs in the device itself. At least two detectors are required: one (tracker), where the primary gamma undergoes a Compton interaction and a second one (calorimeter), in which the scattered gamma is completely absorbed. Eventually the polar angle and hence a "cone" of possible incident directions are obtained (event with "incomplete geometry"). Different solutions for the two detectors are proposed in the literature: our design foresees two similar Position Sensitive Photomultipliers (PMT, Hamamatsu H8500). Each PMT has 64 output channels that are reduced to 4 using a charge multiplexed readout system, i.e. a Series Charge Multiplexing net of resistors. Triggering of the system is provided by the coincidence of fast signals extracted at the last dynode of the PMTs. Assets are the low cost and the simplicity of design and operation, having just one type of device; among drawbacks there is a lower resolution with respect to more sophisticated trackers and full 64 channels Readout. This paper does compare our design of our two-Hamamatsu CC to other solutions and shows how the spatial and energy accuracy is suitable for the inspection of radioactivity in mice.

  7. A double photomultiplier Compton camera and its readout system for mice imaging

    SciTech Connect

    Fontana, Cristiano Lino; Atroshchenko, Kostiantyn; Baldazzi, Giuseppe; Uzunov, Nikolay; Di Domenico, Giovanni

    2013-04-19

    We have designed a Compton Camera (CC) to image the bio-distribution of gamma-emitting radiopharmaceuticals in mice. A CC employs the 'electronic collimation', i.e. a technique that traces the gamma-rays instead of selecting them with physical lead or tungsten collimators. To perform such a task, a CC measures the parameters of the Compton interaction that occurs in the device itself. At least two detectors are required: one (tracker), where the primary gamma undergoes a Compton interaction and a second one (calorimeter), in which the scattered gamma is completely absorbed. Eventually the polar angle and hence a 'cone' of possible incident directions are obtained (event with 'incomplete geometry'). Different solutions for the two detectors are proposed in the literature: our design foresees two similar Position Sensitive Photomultipliers (PMT, Hamamatsu H8500). Each PMT has 64 output channels that are reduced to 4 using a charge multiplexed readout system, i.e. a Series Charge Multiplexing net of resistors. Triggering of the system is provided by the coincidence of fast signals extracted at the last dynode of the PMTs. Assets are the low cost and the simplicity of design and operation, having just one type of device; among drawbacks there is a lower resolution with respect to more sophisticated trackers and full 64 channels Readout. This paper does compare our design of our two-Hamamatsu CC to other solutions and shows how the spatial and energy accuracy is suitable for the inspection of radioactivity in mice.

  8. The cryogenic readout system with GaAs JFETs for multi-pixel cameras

    NASA Astrophysics Data System (ADS)

    Hibi, Y.; Matsuo, H.; Nagata, H.; Ikeda, H.; Fujiwara, M.

    2010-11-01

    Our purpose is to realize a multi-pixel sub-millimeter/terahertz camera with the superconductor - insulator - superconductor photon detectors. These detectors must be cooled below 1 K. Since these detectors have high impedance, signal amplifiers of each pixel must be setting aside of them for precise signal readout. Therefore, it is desirable that the readout system work well even in cryogenic temperature. We selected the n-type GaAs JFETs as cryogenic circuit elements. From our previous studies, the n-type GaAs JFETs have good cryogenic properties even when those power dissipations are low. We have designed several kinds of integration circuits (ICs) and demonstrated their performance at cryogenic temperature. Contents of ICs are following; AC coupled trans-impedance amplifiers, voltage distributors for suppressing input offset voltage of AC coupled CTIAs, multiplexers with sample-and holds, and shift-registers for controlling multiplex timing. The power dissipation of each circuit is 0.5 to 3 micro watts per channel. We also have designed and manufactured 32-channel multi-chip-modules with these ICs. These modules can make 32- channel input photo current signals into one or two serial output voltage signal(s). Size of these is 40mm x 30mm x 2mm and estimated total power dissipation is around 400 micro watts.

  9. Spatial distribution read-out system for thermoluminescence sheets

    NASA Technical Reports Server (NTRS)

    Yamamoto, I.; Tomiyama, T.; Imaeda, K.; Ninagawa, K.; Wada, T.; Yamashita, Y.; Misaki, A.

    1985-01-01

    A spatial distribution read-out system of thermoluminescence (TL) sheets is developed. This system consists of high gain image intensifier, a CCD-TV camera, a video image processor and a host computer. This system has been applied to artificial TL sheets (BaSO4:Eu doped) for detecting high energy electromagnetic shower and heavy nuclei tracks.

  10. A Digital Readout System For The CSO Microwave Kinetic Inductance Camera

    NASA Astrophysics Data System (ADS)

    Max-Moerbeck, Walter; Mazin, B. A.; Zmuidzinas, J.

    2007-12-01

    Submillimeter galaxies are important to the understanding of galaxy formation and evolution. Determination of the spectral energy distribution in the millimeter and submillimeter regimes allows important and powerful diagnostics. Our group is developing a camera for the Caltech Submillimeter Observatory (CSO) using Microwave Kinetic Inductance Detectors (MKIDs). MKIDs are superconducting devices whose impedance changes with the absorption of photons. The camera will have 600 spatial pixels and 4 bands at 750 μm, 850 μm, 1.1 mm and 1.3 mm. For each spatial pixel of the camera the radiation is coupled to the MKIDs using phased-array antennas. This signal is split into 4 different bands using filters and detected using the superconductor as part of a MKID's resonant circuit. The detection process consists of measurement of the changes in the transmission through the resonator when it is illuminated. By designing resonant circuits to have different resonant frequencies and high transmission out resonance, MKIDs can be frequency-domain multiplexed. This allows the simultaneous readout of many detectors through a single coaxial cable. The readout system makes use of microwave IQ modulation and is based on commercial electronics components operating at room temperature. The basic readout has been demonstrated on the CSO. We are working on the implementation of an improved design to be tested on a prototype system with 6x6 pixels and 4 colors next April on the CSO.

  11. DAB: Multiplex and system support features

    NASA Astrophysics Data System (ADS)

    Riley, J. L.

    This Report describes the multiplex and system support features of the Eureka 147/DAB digital audio system. It sets out the requirements of all users along the broadcast chain from service providers and broadcaster through to the listener. The contents of the transmission frame are examined drawing the distinction between the main service multiplex and the provision of control information in a separate fast data channel. The concept of the DAB service structure is introduced and the inherent system flexibility for altering the service arrangement is explained. A wide range of service information features builds on those provided in earlier systems, such as RDS (Radio Data System) and is intended to make it easier for a listener to find any required service and to add a further dimension to audio broadcasting. The choices available to users in all of these areas are examined.

  12. READOUT SYSTEM FOR ARRAYS OF FRISCH-RING CDZNTE DETECTORS.

    SciTech Connect

    CUI, Y.; BOLOTNIKOV, A.E.; CAMARDA, G.S.; DE GERONIMO, G.; O'CONNOR, P.; JAMES, R.B.; KARGAR, A.; HARRISON, M.J.; MCGREGOR, D.S.

    2006-10-29

    Frisch-ring CdZnTe detectors have demonstrated good energy resolution for identifying isotopes, <1% FWHM at 662 keV, and good efficiency for detecting gamma rays. We will fabricate and test at Brookhaven National Laboratory an integrated module of a 64-element array of 6 x 6 x 12 mm{sup 3} Frisch-ring detectors, coupled with a readout electronics system. It supports 64 readout channels, and includes front-end electronics, signal processing circuit, USB interface and high-voltage power supply. The data-acquisition software is used to process the data stream, which includes amplitude and timing information for each detected event. This paper describes the design and assembly of the detector modules, readout electronics, and a conceptual prototype system. Some test results are also reported.

  13. A compact light readout system for longitudinally segmented shashlik calorimeters

    NASA Astrophysics Data System (ADS)

    Berra, A.; Brizzolari, C.; Cecchini, S.; Cindolo, F.; Jollet, C.; Longhin, A.; Ludovici, L.; Mandrioli, G.; Mauri, N.; Meregaglia, A.; Paoloni, A.; Pasqualini, L.; Patrizii, L.; Pozzato, M.; Pupilli, F.; Prest, M.; Sirri, G.; Terranova, F.; Vallazza, E.; Votano, L.

    2016-09-01

    The longitudinal segmentation of shashlik calorimeters is challenged by dead zones and non-uniformities introduced by the light collection and readout system. This limitation can be overcome by direct fiber-photosensor coupling, avoiding routing and bundling of the wavelength shifter fibers and embedding ultra-compact photosensors (SiPMs) in the bulk of the calorimeter. We present the first experimental test of this readout scheme performed at the CERN PS-T9 beamline in 2015 with negative particles in the 1-5 GeV energy range. In this paper, we demonstrate that the scheme does not compromise the energy resolution and linearity compared with standard light collection and readout systems. In addition, we study the performance of the calorimeter for partially contained charged hadrons to assess the e / π separation capability and the response of the photosensors to direct ionization.

  14. Upgrade of the D0 luminosity monitor readout system

    SciTech Connect

    Anderson, John; Bridges, Lloyd; Casey, Brendan; Enari, Yuji; Green, Johnny; Johnson, Marvin; Kwarciany, Rick; Miao, Chyi-Chiang; Partridge, Richard; Yoo, Hwi Dong; Wang, Jigang; /Brown U. /Fermilab

    2006-12-01

    We describe upgrades to the readout system for the D0 Luminosity Monitor. The D0 Luminosity Monitor consists of plastic scintillation detectors with fine-mesh photomultiplier readout that cover the pseudorapidity range 2.7 < |{eta}| < 4.4. The detector is designed to provide a precise measurement of the rate for non-diffractive inelastic collisions that is used to calculate the TeVatron luminosity at D0. The new readout system is based on custom VME electronics that make precise time-of-flight and charge measurements for each luminosity counter. These measurements are used to identify beam crossings with non-diffractive interactions by requiring in-time hits in both the forward and backward luminosity counters. We have also significantly increased signal/noise for the photomultiplier signals by developing a new front-end preamplifier and improving the grounding scheme.

  15. Multiplexed fluorescence detector system for capillary electrophoresis

    DOEpatents

    Yeung, E.S.; Taylor, J.A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  16. Multiplexed fluorescence detector system for capillary electrophoresis

    DOEpatents

    Yeung, Edward S.; Taylor, John A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  17. Multiplexed fluorescence detector system for capillary electrophoresis

    DOEpatents

    Yeung, E.S.; Taylor, J.A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  18. Multiplexed fluorescence detector system for capillary electrophoresis

    DOEpatents

    Yeung, Edward S.; Taylor, John A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  19. Fiber optic multiplex optical transmission system

    NASA Technical Reports Server (NTRS)

    Bell, C. H. (Inventor)

    1977-01-01

    A multiplex optical transmission system which minimizes external interference while simultaneously receiving and transmitting video, digital data, and audio signals is described. Signals are received into subgroup mixers for blocking into respective frequency ranges. The outputs of these mixers are in turn fed to a master mixer which produces a composite electrical signal. An optical transmitter connected to the master mixer converts the composite signal into an optical signal and transmits it over a fiber optic cable to an optical receiver which receives the signal and converts it back to a composite electrical signal. A de-multiplexer is coupled to the output of the receiver for separating the composite signal back into composite video, digital data, and audio signals. A programmable optic patch board is interposed in the fiber optic cables for selectively connecting the optical signals to various receivers and transmitters.

  20. Optimized readout system for cooled optically stimulated luminescence

    NASA Astrophysics Data System (ADS)

    Miller, S. D.; Eschbach, P. A.

    1990-09-01

    Cooled Optically Stimulated Luminescence (COSL) in CaF2:Mn is an ionizing radiation dosimetry method recently developed at the Pacific Northwest Laboratory (PNL). In this method CaF2:Mn crystals irradiated by gamma radiation at room temperature are cooled to liquid nitrogen temperature (77 K), stimulated by ultraviolet laser light at 326 nm, and allowed to warm to room temperature. Light emission proportional to the gamma exposure occurs as the TLD warms from liquid nitrogen temperature to room temperature. The new method is an example of a highly sensitive phototransfer technique which could form the basis for future radiation dosimetry applications. Measurements to date have shown high potential for measuring gamma exposures in the range of 10 microR. The high sensitivity of the COSL technique is due in part to the larger quantum efficiency of radiative recombination at low temperatures and to the complete absence of the incandescent background associated with conventional thermoluminescent readout methods. Along with the potential for a system which is more sensitive than thermoluminescent readers, multiple COSL readouts can be performed with minimal reduction in the COSL intensity. The multiple readout capability can serve as a possible permanent dosimetry record, thus allowing the reanalysis of a questionable reading. In an attempt to optimize the sensitivity of the COSL method, a new readout system is being developed.

  1. Implementation of the Timepix ASIC in the Scalable Readout System

    NASA Astrophysics Data System (ADS)

    Lupberger, M.; Desch, K.; Kaminski, J.

    2016-09-01

    We report on the development of electronics hardware, FPGA firmware and software to provide a flexible multi-chip readout of the Timepix ASIC within the framework of the Scalable Readout System (SRS). The system features FPGA-based zero-suppression and the possibility to read out up to 4×8 chips with a single Front End Concentrator (FEC). By operating several FECs in parallel, in principle an arbitrary number of chips can be read out, exploiting the scaling features of SRS. Specifically, we tested the system with a setup consisting of 160 Timepix ASICs, operated as GridPix devices in a large TPC field cage in a 1 T magnetic field at a DESY test beam facility providing an electron beam of up to 6 GeV. We discuss the design choices, the dedicated hardware components, the FPGA firmware as well as the performance of the system in the test beam.

  2. A novel IPTV program multiplex access system to EPON

    NASA Astrophysics Data System (ADS)

    Xu, Xian; Liu, Deming; He, Wei; Lu, Xi

    2007-11-01

    With the rapid development of high speed networks, such as Ethernet Passive Optical Network (EPON), traffic patterns in access networks have evolved from traditional text-oriented service to the mixed text-, voice- and video- based services, leading to so called "Triple Play". For supporting IPTV service in EPON access network infrastructure, in this article we propose a novel IPTV program multiplex access system to EPON, which enables multiple IPTV program source servers to seamlessly access to IPTV service access port of optical line terminal (OLT) in EPON. There are two multiplex schemes, namely static multiplex scheme and dynamic multiplex scheme, in implementing the program multiplexing. Static multiplex scheme is to multiplex all the IPTV programs and forward them to the OLT, regardless of the need of end-users. While dynamic multiplex scheme can dynamically multiplex and forward IPTV programs according to what the end-users actually demand and those watched by no end-user would not be multiplexed. By comparing these two schemes, a reduced traffic of EPON can be achieved by using dynamic multiplex scheme, especially when most end-users are watching the same few IPTV programs. Both schemes are implemented in our system, with their hardware and software designs described.

  3. Fast Low-Cost Multiple Sensor Readout System

    DOEpatents

    Carter-Lewis, David; Krennich, Frank; Le Bohec, Stephane; Petry, Dirk; Sleege, Gary

    2004-04-06

    A low resolution data acquisition system is presented. The data acquisition system has a plurality of readout modules serially connected to a controller. Each readout module has a FPGA in communication with analog to digital (A/D) converters, which are connected to sensors. The A/D converter has eight bit or lower resolution. The FPGA detects when a command is addressed to it and commands the A/D converters to convert analog sensor data into digital data. The digital data is sent on a high speed serial communication bus to the controller. A graphical display is used in one embodiment to indicate if a sensor reading is outside of a predetermined range.

  4. Performance of Pixel-Readout Micro-Pixel Chamber with Analog-Readout System Used as X-ray Polarimeter

    NASA Astrophysics Data System (ADS)

    Katagiri, Hideaki; Ono, Kenichi; Uchiyama, Hideki; Tsuru, Takeshi Go; Matsumoto, Hironori; Hyodo, Yoshiaki; Kubo, Hidetoshi; Miuchi, Kentaro; Tanimori, Toru

    2007-12-01

    We developed an analog-readout system for a pixel-readout micro-pixel chamber (μ-PIC) to be used as an astronomical X-ray polarimeter, and demonstrated that the sensitivity of the new system reached up to that predicted by a simulation. A pixel-readout μ-PIC is a micro-pattern gaseous detector with a fine position resolution and good stability at sufficient gain operation, and is suited for astronomical X-ray polarimetry. However, as shown by Katagiri et al. (2007), the sensitivity to X-ray polarization was found to be statistically lower by a significant amount than that expected from the simulation of Ueno et al. [Nucl. Instrum. Methods Phys. Res., Sect. A 525 (2004) 28] because of the readout system and background produced by the scattering of the X-ray beam in air. We therefore developed a new readout system and carried out a beam test with aluminum tubes that reduced the background. As a result, we demonstrated that for collimated beams, the modulation factors, which are indicators of the sensitivity to X-ray polarization, were 0.24± 0.08 at 8 keV and 0.18± 0.07 at 15 keV in a neon-based gas mixture, and 0.18± 0.04 at 15 keV in an argon-based mixture. These values are consistent with those predicted by the simulation within errors.

  5. Development of the rf-SQUID Based Multiplexing System for the HOLMES Experiment

    NASA Astrophysics Data System (ADS)

    Puiu, A.; Becker, D.; Bennett, D.; Faverzani, M.; Ferri, E.; Fowler, J.; Gard, J.; Hays-Wehle, J.; Hilton, G.; Giachero, A.; Maino, M.; Mates, J.; Nucciotti, A.; Schmidt, D.; Swetz, D.; Ullom, J.; Vale, L.

    2016-07-01

    Measuring the neutrino mass is one of the most compelling issues in particle physics. The European Research Council has funded HOLMES, a new experiment for a direct measurement of neutrino mass that started in 2014. HOLMES will perform a precise measurement of the end point of the Electron Capture decay spectrum of ^{163}Ho in order to extract information on neutrino mass with a sensitivity as low as 0.4 eV. HOLMES, in its final configuration, will deploy a 1000 pixel array of low-temperature microcalorimeters: each calorimeter consists of an absorber, where the Ho atoms will be implanted, coupled to a transition edge sensor thermometer. The read out for an array of 1000 cryogenic detectors is a crucial matter: for HOLMES, a special radio-frequency-based multiplexing system is being developed. In this contribution, we outline the performance and special features of the multiplexing system and readout methods chosen for HOLMES.

  6. Performance boundaries for prioritized multiplexing systems

    NASA Technical Reports Server (NTRS)

    Clare, Loren P.; Rubin, Izhak

    1987-01-01

    Systems in which many data sources are multiplexed over a single communication channel are considered. Data from all the sources are generated in fixed-length packets and are stored in a common buffer with finite capacity. Packets that overflowed or were removed from the buffer prior to transmission are lost. The system performance measure is the set of packet loss probabilities associated with the sources. Queueing disciplines vary depending on the stringency of prioritization and the utilization of the system resources. The set of all possible performances is characterized as the set of all queueing disciplines is spanned. Whether a given performance is possible can be deduced. Strategies that achieve the minimum overall loss probability are identified. The extreme disciplines are specified, and their performances are calculable by means of a given algorithm.

  7. On the design of prioritized multiplexing systems

    NASA Technical Reports Server (NTRS)

    Clare, L. P.; Rubin, I.

    1983-01-01

    Systems in which many data sources are multiplexed over a single communication channel are considered. Data from all the sources are generated in fixed-length packets, and are stored in a common buffer with finite capacity. Packets overflowed or removed from the buffer prior to transmission are lost. The system performance measure is the set of packet loss probabilities associated with the sources. Queueing disciplines vary depending on the stringency of prioritization and the utilization of system resources. The set of all possible performances is characterized as the set of all queueing disciplines is spanned. Whether a given performance is possible can be deduced. Strategies that achieve the minimum overall loss probability are identified. The extreme disciplines are specified, and their performances are calculable by means of a given algorithm.

  8. The LCLS Undulator Beam Loss Monitor Readout System

    SciTech Connect

    Dusatko, John; Browne, M.; Fisher, A.S.; Kotturi, D.; Norum, S.; Olsen, J.; /SLAC

    2012-07-23

    The LCLS Undulator Beam Loss Monitor System is required to detect any loss radiation seen by the FEL undulators. The undulator segments consist of permanent magnets which are very sensitive to radiation damage. The operational goal is to keep demagnetization below 0.01% over the life of the LCLS. The BLM system is designed to help achieve this goal by detecting any loss radiation and indicating a fault condition if the radiation level exceeds a certain threshold. Upon reception of this fault signal, the LCLS Machine Protection System takes appropriate action by either halting or rate limiting the beam. The BLM detector consists of a PMT coupled to a Cherenkov radiator located near the upstream end of each undulator segment. There are 33 BLMs in the system, one per segment. The detectors are read out by a dedicated system that is integrated directly into the LCLS MPS. The BLM readout system provides monitoring of radiation levels, computation of integrated doses, detection of radiation excursions beyond set thresholds, fault reporting and control of BLM system functions. This paper describes the design, construction and operational performance of the BLM readout system.

  9. Upgrade of the MAGIC Telescope with a Multiplexed Fiber-Optic 2GSamples/s FADC Data Acquisition System system

    NASA Astrophysics Data System (ADS)

    Goebel, Florian; Bartko, Hendrik; Carmona, Emiliano; Galante, Nicola; Jogler, Tobias; Mirzoyan, Razmik; Coarasa, Jose Antonio; Teshima, Masahiro

    In February 2007 the MAGIC Air Cherenkov Telescope for gamma ray astronomy was fully upgraded with a ultra fast 2GSamples/s digitization system. Since the gamma ray signals are very short, a fast readout can minimize the influence of the background from the light of the night sky. Fast flash analog to digital converters (FADCs) are commercially available, but they are prohibitively expensive and power consuming for the MAGIC camera which contains 576 pixels. The upgraded readout system uses a novel technique of fiber-optic multiplexing which uses 10 bit 2Gsamples/s FADCs to digitize 16 channels consecutively and optical fibers to delay the analog signals. A distributed data acquisition system using GBit Ethernet and FiberChannel allows to read out the 100 kB events with a continuous rate of up to 1 kHz.

  10. Cryogenic low noise and low dissipation multiplexing electronics, using HEMT+SiGe ASICs, for the readout of high impedance sensors: New version

    NASA Astrophysics Data System (ADS)

    de la Broïse, Xavier; Lugiez, Francis; Bounab, Ayoub; Le Coguie, Alain

    2015-07-01

    High Electron Mobility Transistors (HEMTs), optimized by CNRS/LPN laboratory for ultra-low noise at very low temperature, have demonstrated their capacity to be used in place of Si JFETs when working temperatures below 100 K are required. We associated them with specific SiGe ASICs that we developed, to implement a complete readout channel able to read highly segmented high impedance detectors within a framework of very low thermal dissipation. Our electronics is dimensioned to read 4096 detection channels, of typically 1 MΩ impedance, and performs 32:1 multiplexing and amplifying, dissipating only 6 mW at 2.5 K and 100 mW at 15 K thanks to high impedance commuting of input stage, with a typical noise of 1 nV/√Hz at 1 kHz.

  11. Trigger and Readout System for the Ashra-1 Detector

    NASA Astrophysics Data System (ADS)

    Aita, Y.; Aoki, T.; Asaoka, Y.; Morimoto, Y.; Motz, H. M.; Sasaki, M.; Abiko, C.; Kanokohata, C.; Ogawa, S.; Shibuya, H.; Takada, T.; Kimura, T.; Learned, J. G.; Matsuno, S.; Kuze, S.; Binder, P. M.; Goldman, J.; Sugiyama, N.; Watanabe, Y.

    Highly sophisticated trigger and readout system has been developed for All-sky Survey High Resolution Air-shower (Ashra) detector. Ashra-1 detector has 42 degree diameter field of view. Detection of Cherenkov and fluorescence light from large background in the large field of view requires finely segmented and high speed trigger and readout system. The system is composed of optical fiber image transmission system, 64 × 64 channel trigger sensor and FPGA based trigger logic processor. The system typically processes the image within 10 to 30 ns and opens the shutter on the fine CMOS sensor. 64 × 64 coarse split image is transferred via 64 × 64 precisely aligned optical fiber bundle to a photon sensor. Current signals from the photon sensor are discriminated by custom made trigger amplifiers. FPGA based processor processes 64 × 64 hit pattern and correspondent partial area of the fine image is acquired. Commissioning earth skimming tau neutrino observational search was carried out with this trigger system. In addition to the geometrical advantage of the Ashra observational site, the excellent tau shower axis measurement based on the fine imaging and the night sky background rejection based on the fine and fast imaging allow zero background tau shower search. Adoption of the optical fiber bundle and trigger LSI realized 4k channel trigger system cheaply. Detectability of tau shower is also confirmed by simultaneously observed Cherenkov air shower. Reduction of the trigger threshold appears to enhance the effective area especially in PeV tau neutrino energy region. New two dimensional trigger LSI was introduced and the trigger threshold was lowered. New calibration system of the trigger system was recently developed and introduced to the Ashra detector

  12. An OS9-UNIX data acquisition system with ECL readout

    SciTech Connect

    Ziem, P.; Beschorner, C.; Bohne, W.; Drescher, B.; Friese, T.; Kiehne, T.; Kluge, C.

    1996-02-01

    A new data acquisition system has been developed at the Hahn-Meitner-Institut to handle almost 550 parameters of nuclear physics experiments. The system combines a UNIX host running a portable data buffer router and a VME front-end based on the OS9 real time operating system. Different kinds of pulse analyzers are located in several CAMAC crates which are controlled by the VME system via a VICbus connection. Data readout is performed by means of an ECL daisy chain. Besides controlling CAMAC the main purpose of the VME front-end is event data formatting and histogramming. Using TCP/IP services, the UNIX host receives formatted data packages for data storage and display. During a beam time at the antiproton accelerator LEAR/CERN, the PS208 experiment has accumulated about 100 Gbyte of event data.

  13. Readout system of TPC/MPD NICA project

    SciTech Connect

    Averyanov, A. V.; Bajajin, A. G.; Chepurnov, V. F.; Cheremukhina, G. A.; Fateev, O. V.; Korotkova, A. M.; Levchanovskiy, F. V.; Lukstins, J.; Movchan, S. A.; Razin, S. V.; Rybakov, A. A.; Vereschagin, S. V. Zanevsky, Yu. V.; Zaporozhets, S. A.; Zruyev, V. N.

    2015-12-15

    The time-projection chamber (TPC) is the main tracking detector in the MPD/NICA. The information on charge-particle tracks in the TPC is registered by the MWPG with cathode pad readout. The frontend electronics (FEE) are developed with use of modern technologies such as application specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), and data transfer to a concentrator via a fast optical interface. The main parameters of the FEE are as follows: total number of channels, ∼95 000; data stream from the whole TPC, 5 GB/s; low power consumption, less than 100 mW/ch; signal to noise ratio (S/N), 30; equivalent noise charge (ENC), <1000e{sup –} (C{sub in} = 10–20 pF); and zero suppression (pad signal rejection ∼90%). The article presents the status of the readout chamber construction and the data acquisition system. The results of testing FEE prototypes are presented.

  14. Transmission of multiplexed video signals in multimode optical fiber systems

    NASA Technical Reports Server (NTRS)

    White, Preston, III

    1988-01-01

    Kennedy Space Center has the need for economical transmission of two multiplexed video signals along multimode fiberoptic systems. These systems must span unusual distances and must meet RS-250B short-haul standards after reception. Bandwidth is a major problem and studies of the installed fibers, available LEDs and PINFETs led to the choice of 100 MHz as the upper limit for the system bandwidth. Optical multiplexing and digital transmission were deemed inappropriate. Three electrical multiplexing schemes were chosen for further study. Each of the multiplexing schemes included an FM stage to help meet the stringent S/N specification. Both FM and AM frequency division multiplexing methods were investigated theoretically and these results were validated with laboratory tests. The novel application of quadrature amplitude multiplexing was also considered. Frequency division multiplexing of two wideband FM video signal appears the most promising scheme although this application requires high power highly linear LED transmitters. Futher studies are necessary to determine if LEDs of appropriate quality exist and to better quantify performance of QAM in this application.

  15. Multiplexed operation of nanoelectromechanical systems (NEMS) arrays

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwinkumar

    Nanoelectromechanical systems (NEMS) are rapidly being developed for a variety of applications as well as for exploring interesting regimes in fundamental physics. In most of these endeavors, operation of a NEMS device involves actuating the device harmonically around its fundamental resonance and detecting the subsequent motion, while the device interacts with its environment. Up to date, with few notable exceptions, most NEMS work has been done on single devices. Even though a single NEMS resonator is exceptionally sensitive, a typical application, be it nanomechanical sensing or signal processing, requires the detection of signals from many resonators distributed over the surface of a chip. Therefore, one of the key technological challenges in the field of NEMS is the development of multiplexed measurement techniques to simultaneously detect the motion of a large number of NEMS resonators. The main objective of this project is to address this important and difficult problem of interfacing with a large number of NEMS devices and facilitating the use of such arrays in, for example, sensing and signal processing applications. In preparatory work, photothermal/optical operation of high frequency NEMS at ambient conditions is carried out. The flexural resonances of bi-layered doubly clamped beams are actuated using a tightly focused modulated laser source and the resulting out-of-plane displacements are measured at a single point using a path-stabilized interferometer. The signal from the interferometer is coupled to a radiofrequency (RF) lock-in amplifier allowing for narrowband phase-sensitive detection. The optical measurements have enabled the determination of beam resonance parameters such as resonance frequencies, mechanical quality (Q) factors and mode shapes. Experimental results show that the all-optical excitation-detection technique is well suited for the non-destructive testing and remote interrogation of NEMS with exquisite spatial and temporal

  16. Merlin: a fast versatile readout system for Medipix3

    NASA Astrophysics Data System (ADS)

    Plackett, R.; Horswell, I.; Gimenez, E. N.; Marchal, J.; Omar, D.; Tartoni, N.

    2013-01-01

    This contribution reports on the development of a new high rate readout system for the Medipix3 hybrid pixel ASIC developed by the Detector Group at Diamond Light Source. It details the current functionality of the system and initial results from tests on Diamond's B16 beamline. The Merlin system is based on a National Instruments PXI/FlexRIO system running a Xilinx Virtex5 FPGA. It is capable of recording Medipix3 256 by 256 by 12 bit data frames at over 1 kHz in bursts of 1200 frames and running at over 100 Hz continuously to disk or over a TCP/IP link. It is compatible with the standard Medipix3 single chipboards developed at CERN and is capable of driving them over cable lengths of up to 10 m depending on the data rate required. In addition to a standalone graphical interface, a system of remote TCP/IP control and data transfer has been developed to allow easy integration with third party control systems and scripting languages. Two Merlin systems are being deployed on the B16 and I16 beamlines at Diamond and the system has been integrated with the EPICS/GDA control systems used. Results from trigger synchronisation, fast burst and high rate tests made on B16 in March are reported and demonstrate an encouraging reliability and timing accuracy. In addition to normal high resolution imaging applications of Medipix3, the results indicate the system could profitably be used in `pump and probe' style experiments, where a very accurate, high frame rate is especially beneficial. In addition to these two systems, Merlin is being used by the Detector Group to test the Excalibur 16 chip hybrid modules, and by the LHCb VELO Pixel Upgrade group in their forthcoming testbeams. Additionally the contribution looks forward to further developments and improvements in the system, including full rate quad chip readout capability, multi-FPGA support, long distance optical communication and further functionality enhancements built on the capabilities of the Medipix3 chips.

  17. Small-Scale Readout Systems Prototype for the STAR PIXEL Detector

    SciTech Connect

    Szelezniak, Michal A.; Besson, Auguste; Colledani, Claude; Dorokhov, Andrei; Dulinski, Wojciech; Greiner, Leo C.; Himmi, Abdelkader; Hu, Christine; Matis, Howard S.; Ritter, Hans Georg; Rose, Andrew; Shabetai, Alexandre; Stezelberger, Thorsten; Sun, Xiangming; Thomas, Jim H.; Valin, Isabelle; Vu, Chinh Q.; Wieman, Howard H.; Winter, Marc

    2008-10-01

    A prototype readout system for the STAR PIXEL detector in the Heavy Flavor Tracker (HFT) vertex detector upgrade is presented. The PIXEL detector is a Monolithic Active Pixel Sensor (MAPS) based silicon pixel vertex detector fabricated in a commercial CMOS process that integrates the detector and front-end electronics layers in one silicon die. Two generations ofMAPS prototypes designed specifically for the PIXEL are discussed. We have constructed a prototype telescope system consisting of three small MAPS sensors arranged in three parallel and coaxial planes with a readout system based on the readout architecture for PIXEL. This proposed readout architecture is simple and scales to the size required to readout the final detector. The real-time hit finding algorithm necessary for data rate reduction in the 400 million pixel detector is described, and aspects of the PIXEL system integration into the existing STAR framework are addressed. The complete system has been recently tested and shown to be fully functional.

  18. The readout system for the ArTeMis camera

    NASA Astrophysics Data System (ADS)

    Doumayrou, E.; Lortholary, M.; Dumaye, L.; Hamon, G.

    2014-07-01

    During ArTeMiS observations at the APEX telescope (Chajnantor, Chile), 5760 bolometric pixels from 20 arrays at 300mK, corresponding to 3 submillimeter focal planes at 450μm, 350μm and 200μm, have to be read out simultaneously at 40Hz. The read out system, made of electronics and software, is the full chain from the cryostat to the telescope. The readout electronics consists of cryogenic buffers at 4K (NABU), based on CMOS technology, and of warm electronic acquisition systems called BOLERO. The bolometric signal given by each pixel has to be amplified, sampled, converted, time stamped and formatted in data packets by the BOLERO electronics. The time stamping is obtained by the decoding of an IRIG-B signal given by APEX and is key to ensure the synchronization of the data with the telescope. Specifically developed for ArTeMiS, BOLERO is an assembly of analogue and digital FPGA boards connected directly on the top of the cryostat. Two detectors arrays (18*16 pixels), one NABU and one BOLERO interconnected by ribbon cables constitute the unit of the electronic architecture of ArTeMiS. In total, the 20 detectors for the tree focal planes are read by 10 BOLEROs. The software is working on a Linux operating system, it runs on 2 back-end computers (called BEAR) which are small and robust PCs with solid state disks. They gather the 10 BOLEROs data fluxes, and reconstruct the focal planes images. When the telescope scans the sky, the acquisitions are triggered thanks to a specific network protocol. This interface with APEX enables to synchronize the acquisition with the observations on sky: the time stamped data packets are sent during the scans to the APEX software that builds the observation FITS files. A graphical user interface enables the setting of the camera and the real time display of the focal plane images, which is essential in laboratory and commissioning phases. The software is a set of C++, Labview and Python, the qualities of which are respectively used

  19. Development of a read out driver for ATLAS micromegas based on the Scalable Readout System

    NASA Astrophysics Data System (ADS)

    Zibell, A.

    2014-01-01

    With future LHC luminosity upgrades, part of the ATLAS muon spectrometer has to be changed, to cope with the increased flux of uncorrelated neutron and gamma particles. Micromegas detectors were chosen as precision tracker for the New Small Wheels, that will replace the current Small Wheel muon detector stations during the LHC shutdown foreseen for 2018. To read out these detectors together with all other ATLAS subsystems, a readout driver was developed to integrate these micromegas detectors into the ATLAS data acquisition infrastructure. The readout driver is based on the Scalable Readout System, and its tasks include trigger handling, slow control, event building and data transmission to the high-level readout systems. This article describes the layout and functionalities of this readout driver and its components, as well as a test of its functionalities in the cosmic ray facility of Ludwig-Maximilians University Munich.

  20. Optical readout in a multi-module system test for the ATLAS pixel detector

    NASA Astrophysics Data System (ADS)

    Flick, Tobias; Becks, Karl-Heinz; Gerlach, Peter; Kersten, Susanne; Mättig, Peter; Nderitu Kirichu, Simon; Reeves, Kendall; Richter, Jennifer; Schultes, Joachim

    2006-09-01

    The innermost part of the ATLAS experiment at the LHC, CERN, will be a pixel detector, which is presently under construction. The command messages and the readout data of the detector are transmitted over an optical data path. The readout chain consists of many components which are produced at several locations around the world, and must work together in the pixel detector. To verify that these parts are working together as expected a system test has been built up. It consists of detector modules, optoboards, optical fibres, Back of Crate cards, Readout Drivers, and control computers. In this paper, the system test setup and the operation of the readout chain are described. Also, some results of tests using the final pixel detector readout chain are given.

  1. Toward a Reduced-Wire Readout System for Ultrasound Imaging

    PubMed Central

    Lim, Jaemyung; Arkan, Evren F.; Degertekin, F. Levent; Ghovanloo, Maysam

    2015-01-01

    We present a system-on-a-chip (SoC) for use in high-frequency capacitive micromachined ultrasonic transducer (CMUT) imaging systems. This SoC consists of trans-impedance amplifiers (TIA), delay locked loop (DLL) based clock multiplier, quadrature sampler, and pulse width modulator (PWM). The SoC down converts RF echo signal to baseband by quadrature sampling which facilitates modulation. To send data through a 1.6 m wire in the catheter which has limited bandwidth and is vulnerable to noise, the SoC creates a pseudo-digital PWM signal which can be used for back telemetry or wireless readout of the RF data. In this implementation, using a 0.35-μm std. CMOS process, the TIA and single-to-differential (STD) converter had 45 MHz bandwidth, the quadrature sampler had 10.1 dB conversion gain, and the PWM had 5-bit ENoB. Preliminary results verified front-end functionality, and the power consumption of a TIA, STD, quadrature sampler, PWM, and clock multiplier was 26 mW from a 3 V supply. PMID:25571135

  2. Electronics for a Next-Generation SQUID-Based Time-Domain Multiplexing System

    SciTech Connect

    Reintsema, C. D.; Doriese, W. R.; Hilton, G. C.; Irwin, K. D.; Krinsky, J. W.; Adams, J. S.; Baker, R.; Bandler, S. R.; Kelly, R. L.; Kilbourne, C. A.; Porter, F. S.; Figueroa-Feliciano, E.; Wikus, P.

    2009-12-16

    A decade has elapsed since the design, development and realization of a SQUID-based time-division multiplexer at NIST. During this time the system has been used extensively for low-temperature-detector-array measurements. Concurrently, there have been substantial advancements both in detector array and commercial electronic component technology. The relevance and applicability of the technology has blossomed as well, often accompanied by more demanding measurement requirements. These factors have motivated a complete redesign of the NIST room-temperature read-out electronics. The redesign has leveraged advancements in component technology to achieve new capabilities better suited to the SQUID multiplexers and detector arrays being realized today. As examples of specific performance enhancements, the overall system bandwidth has been increased by a factor of four (a row switching rate of 6.24 MHz), the compactness has been increased by over a factor of two (a higher number of detector columns and rows per circuit board), and there are two high speed outputs per column (allowing fast switching of SQUID offsets in addition to digital feedback). The system architecture, design implementations, and performance advantages of the new system will be discussed. As an application example, the science chain flight electronics for the Micro-X High Resolution Microcalorimeter X-ray Imaging Rocket will be described as both a motivation for, and a direct implementation of the new system.

  3. A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples.

    PubMed

    Chen, Yiping; Sun, Jiashu; Xianyu, Yunlei; Yin, Binfeng; Niu, Yajing; Wang, Songbai; Cao, Fengjing; Zhang, Xiaoqing; Wang, Yu; Jiang, Xingyu

    2016-08-18

    Even though the gold lateral flow test (GLFT) is low-cost and allows for point-of-care testing (POCT), its intrinsic limitations including low sensitivity and incapability of quantification significantly hinder the clinical application of GLFT for assaying disease biomarkers. To improve the performance of the GLFT without sacrificing its simplicity, we develop a chemiluminescent-gold lateral flow test (C-mode GLFT) for quantitative and multiplex detection of disease biomarkers with an ultrahigh sensitivity at a picomolar level. Horseradish peroxidase (HRP) and antibody (Ab) are simultaneously labeled onto the surface of gold nanoparticles (AuNPs) to achieve a dual-readout (chemiluminescent and visual, C&V-mode GLFT). A red color appears at the test line caused by the accumulation of captured AuNPs in the presence of targets, while HRP on the surface of AuNPs catalyzes the chemiluminescence reaction of luminol to amplify the signal. C-mode GLFT is successfully used for detecting tumor biomarkers (alpha fetoprotein, AFP, and carcino embryonic antigen, CEA) and bacterial infection biomarkers (procalcitonin, PCT) in serum samples as well as whole blood. The excellent features of C-mode GLFT such as straightforward operation, ultrahigh sensitivity and quantitative detection, make it a promising platform for POCT of a variety of disease biomarkers in real samples. PMID:27375054

  4. X-ray and gamma ray detector readout system

    DOEpatents

    Tumer, Tumay O; Clajus, Martin; Visser, Gerard

    2010-10-19

    A readout electronics scheme is under development for high resolution, compact PET (positron emission tomography) imagers based on LSO (lutetium ortho-oxysilicate, Lu.sub.2SiO.sub.5) scintillator and avalanche photodiode (APD) arrays. The key is to obtain sufficient timing and energy resolution at a low power level, less than about 30 mW per channel, including all required functions. To this end, a simple leading edge level crossing discriminator is used, in combination with a transimpedance preamplifier. The APD used has a gain of order 1,000, and an output noise current of several pA/ Hz, allowing bipolar technology to be used instead of CMOS, for increased speed and power efficiency. A prototype of the preamplifier and discriminator has been constructed, achieving timing resolution of 1.5 ns FWHM, 2.7 ns full width at one tenth maximum, relative to an LSO/PMT detector, and an energy resolution of 13.6% FWHM at 511 keV, while operating at a power level of 22 mW per channel. Work is in progress towards integration of this preamplifier and discriminator with appropriate coincidence logic and amplitude measurement circuits in an ASIC suitable for a high resolution compact PET instrument. The detector system and/or ASIC can also be used for many other applications for medical to industrial imaging.

  5. Wavelength division multiplexing. [in fiber optics communication systems

    NASA Technical Reports Server (NTRS)

    Hendricks, H. D.

    1985-01-01

    Wavelength division multiplexing (WDM) represents an approach for expanding the communication capacity and for implementing special data techniques in a fiber optics system. This technology is implemented by adding optical sources of different wavelengths at optical transmitting locations. The present paper is concerned with some of the current efforts in WDM. WDM applications are related to long haul communications, local area data networks, spacecraft and aircraft data systems, fault tolerant computer networks, special sensor devices, high speed data processors, closed circuit and cable television, and submarine cable systems. Attention is given to the current state of wavelength division multiplexing applications, the availability and status of WDM components semiconductor lasers/transmitters, availability and status of fiber optic detectors/receivers, optical fibers/cables/connectors/taps/star couplers, wavelength multiplexers/demultiplexers, and future WDM for local area networks.

  6. A Triggerless readout system for the bar PANDA electromagnetic calorimeter

    NASA Astrophysics Data System (ADS)

    Tiemens, M.; PANDA Collaboration

    2015-02-01

    One of the physics goals of the future bar PANDA experiment at FAIR is to research newly discovered exotic states. Because the detector response created by these particles is very similar to the background channels, a new type of data readout had to be developed, called "triggerless" readout. In this concept, each detector subsystem preprocesses the signal, so that in a later stage, high-level phyiscs constraints can be applied to select events of interest. A dedicated clock source using a protocol called SODANET over optical fibers ensures proper synchronisation between the components. For this new type of readout, a new way of simulating the detector response also needed to be developed, taking into account the effects of pile-up caused by the 20 MHz interaction rate.

  7. The PCIe-based readout system for the LHCb experiment

    NASA Astrophysics Data System (ADS)

    Cachemiche, J. P.; Duval, P. Y.; Hachon, F.; Le Gac, R.; Réthoré, F.

    2016-02-01

    The LHCb experiment is designed to study differences between particles and anti-particles as well as very rare decays in the beauty and charm sector at the LHC. The detector will be upgraded in 2019 in order to significantly increase its efficiency, by removing the first-level hardware trigger. The upgrade experiment will implement a trigger-less readout system in which all the data from every LHC bunch-crossing are transported to the computing farm over 12000 optical links without hardware filtering. The event building and event selection are carried out entirely in the farm. Another original feature of the system is that data transmitted through these fibres arrive directly to computers through a specially designed PCIe card called PCIe40. The same board handles the data acquisition flow and the distribution of fast and slow controls to the detector front-end electronics. It embeds one of the most powerful FPGAs currently available on the market with 1.2 million logic cells. The board has a bandwidth of 480 Gbits/s in both input and output over optical links and 100 Gbits/s over the PCI Express bus to the CPU. We will present how data circulate through the board and in the PC server for achieving the event building. We will focus on specific issues regarding the design of such a board with a very large FPGA, in particular in terms of power supply dimensioning and thermal simulations. The features of the board will be detailed and we will finally present the first performance measurements.

  8. Compact reflection holographic recording system with high angle multiplexing

    NASA Astrophysics Data System (ADS)

    Kanayasu, Mayumi; Yamada, Takehumi; Takekawa, Shunsuke; Akieda, Kensuke; Goto, Akiyo; Yamamoto, Manabu

    2011-02-01

    Holographic memory systems have been widely researched since 1963. However, the size of the drives required and the deterioration of reconstructed data resulting from shrinkage of the medium have made practical use of a hologram memory difficult. In light of this, we propose a novel holographic recording/reconstructing system: a dual-reference beam reflection system that is smaller than conventional systems such as the off-axis or co-axis types, and which is expected to increase the number of multiplexing in angle multiplexed recording. In this multiplex recording system, two laser beams are used as reference beams, and the recorded data are reconstructed stably, even if there is shrinkage of the recording medium. In this paper, a reflection holographic memory system is explained in detail. In addition, the change in angle selectivity resulting from shrinkage of the medium is analyzed using the laminated film three-dimensional simulation method. As a result, we demonstrate that a dual-reference beam multiplex recording system is effective in reducing the influence of medium shrinkage.

  9. Low-background direct readout array performance

    NASA Technical Reports Server (NTRS)

    Goebel, J. H.; Mckelvey, M. E.; Mccreight, C. R.; Anderson, G. M.

    1986-01-01

    The development and evaluation of an integrated array of antimony-doped silicon detectors is described. The spectral range of extrinsic silicon-integrated arrays useful for low-background IR astronomical applications is extended to about 31 microns with this development. The 58 x 62-element array is accessed by a direct readout multiplexer. The device is evaluated with a flexible microcomputer-based drive and readout electronics system in a low-background test dewar. Acceptance testing indicates single-pixel NEPs in the mid-10 to the -18th W/sq rt Hz range, and good global uniformity statistics.

  10. Super-framing technique based on digital readout for infrared thermal image systems

    NASA Astrophysics Data System (ADS)

    Yao, Libin

    2014-11-01

    Photo current integration is the key to improve the performance of a thermal imaging system. By integrating the photo current in the capacitance, the signal-to-noise ratio of the system is greatly improved. Theoretically, the maximum integration time of a thermal imaging system is the frame time of the system. However, due to the low well capacity of the readout integrated circuit, the integration time of the photo current is often shorter than the frame time of the system. To increase the well capacity of the readout circuit is one of the main research topics in thermal imaging system. The super-framing technique can overcome the restriction by reading-out the detector signal in a rate higher than the frame rate and then integrating the signal outside the IRFPA. As the signal integration is carried out outside the pixel, the integration time is no longer restricted. The key of the super-framing technique is the transmission of the signal in high readout rate. The digital readout circuit by integrating the analog-to-digital converter (ADC) array on the readout circuit chip becomes more and more popular with the development of CMOS technologies. Since the digital signal can be transferred outside the chip in a GS/s rate without any concern on noise and distortion, the super-framing technique based on digital readout circuit is advantageous over the analog solution.

  11. A new readout control system for the LHCb upgrade at CERN

    NASA Astrophysics Data System (ADS)

    Alessio, F.; Jacobsson, R.

    2012-11-01

    The LHCb experiment has proposed an upgrade towards a full 40 MHz readout system in order to run between five and ten times its initial design luminosity. The entire readout architecture will be upgraded in order to cope with higher sub-detector occupancies, higher rate and higher network load. In this paper, we describe the architecture, functionalities and a first hardware implementation of a new fast Readout Control system for the LHCb upgrade, which will be entirely based on FPGAs and bi-directional links. We also outline the real-time implementations of the new Readout Control system, together with solutions on how to handle the synchronous distribution of timing and synchronous information to the complex upgraded LHCb readout architecture. One section will also be dedicated to the control and usage of the newly developed CERN GBT chipset to transmit fast and slow control commands to the upgraded LHCb Front-End electronics. At the end, we outline the plans for the deployment of the system in the global LHCb upgrade readout architecture.

  12. Digital radiography using amorphous selenium: photoconductively activated switch (PAS) readout system.

    PubMed

    Reznik, Nikita; Komljenovic, Philip T; Germann, Stephen; Rowlands, John A

    2008-03-01

    A new amorphous selenium (a-Se) digital radiography detector is introduced. The proposed detector generates a charge image in the a-Se layer in a conventional manner, which is stored on electrode pixels at the surface of the a-Se layer. A novel method, called photoconductively activated switch (PAS), is used to read out the latent x-ray charge image. The PAS readout method uses lateral photoconduction at the a-Se surface which is a revolutionary modification of the bulk photoinduced discharge (PID) methods. The PAS method addresses and eliminates the fundamental weaknesses of the PID methods--long readout times and high readout noise--while maintaining the structural simplicity and high resolution for which PID optical readout systems are noted. The photoconduction properties of the a-Se surface were investigated and the geometrical design for the electrode pixels for a PAS radiography system was determined. This design was implemented in a single pixel PAS evaluation system. The results show that the PAS x-ray induced output charge signal was reproducible and depended linearly on the x-ray exposure in the diagnostic exposure range. Furthermore, the readout was reasonably rapid (10 ms for pixel discharge). The proposed detector allows readout of half a pixel row at a time (odd pixels followed by even pixels), thus permitting the readout of a complete image in 30 s for a 40 cm x 40 cm detector with the potential of reducing that time by using greater readout light intensity. This demonstrates that a-Se based x-ray detectors using photoconductively activated switches could form a basis for a practical integrated digital radiography system. PMID:18404939

  13. Remote (250 km) Fiber Bragg Grating Multiplexing System

    PubMed Central

    Fernandez-Vallejo, Montserrat; Rota-Rodrigo, Sergio; Lopez-Amo, Manuel

    2011-01-01

    We propose and demonstrate two ultra-long range fiber Bragg grating (FBG) sensor interrogation systems. In the first approach four FBGs are located 200 km from the monitoring station and a signal to noise ratio of 20 dB is obtained. The second improved version is able to detect the four multiplexed FBGs placed 250 km away, offering a signal to noise ratio of 6–8 dB. Consequently, this last system represents the longest range FBG sensor system reported so far that includes fiber sensor multiplexing capability. Both simple systems are based on a wavelength swept laser to scan the reflection spectra of the FBGs, and they are composed by two identical-lengths optical paths: the first one intended to launch the amplified laser signal by means of Raman amplification and the other one is employed to guide the reflection signal to the reception system. PMID:22164101

  14. Remote (250 km) fiber Bragg grating multiplexing system.

    PubMed

    Fernandez-Vallejo, Montserrat; Rota-Rodrigo, Sergio; Lopez-Amo, Manuel

    2011-01-01

    We propose and demonstrate two ultra-long range fiber Bragg grating (FBG) sensor interrogation systems. In the first approach four FBGs are located 200 km from the monitoring station and a signal to noise ratio of 20 dB is obtained. The second improved version is able to detect the four multiplexed FBGs placed 250 km away, offering a signal to noise ratio of 6-8 dB. Consequently, this last system represents the longest range FBG sensor system reported so far that includes fiber sensor multiplexing capability. Both simple systems are based on a wavelength swept laser to scan the reflection spectra of the FBGs, and they are composed by two identical-lengths optical paths: the first one intended to launch the amplified laser signal by means of Raman amplification and the other one is employed to guide the reflection signal to the reception system. PMID:22164101

  15. Imaging through turbulence with temporally and spatially multiplexed systems

    NASA Astrophysics Data System (ADS)

    Vdovin, Gleb; Soloviev, Oleg; Loktev, Mikhail; Savenko, Slava

    2011-11-01

    Based on the analysis of factors that influence atmospheric imaging over long turbulent horizontal paths, we consider a number of practical configurations of opto-electronic surveillance systems with optimized performance. Our approach is based on simultaneous quasi real-time processing of a number of images obtained through uncorrelated atmospheric paths, using either temporal or spatial multiplexing. Practical results obtained on a 4.25 km imaging path using newly developed imaging system, based on temporal multiplexing, combined with image restoration based on projection on convex sets, are reported. Potential applications include optical and IR long-range security and military surveillance, unmanned aircraft imaging systems and naval optical imaging and warning systems.

  16. Research on copying system of dynamic multiplex holographic stereograms

    NASA Astrophysics Data System (ADS)

    Fu, Huaiping; Yang, Hong; Zheng, Tong

    2003-05-01

    The most important advantage of holographic stereograms over conventional hologram is that they can produce 3D images at any desired scale with movement, holographers in many countries involved in the studies towards it. We began our works in the early 80's and accomplished two research projects automatic system for making synthetic holograms and multiplex synthetic rainbow holograms, Based on these works, a large scale holographic stereogram of an animated goldfish was made by us for practical advertisement. In order to meet the needs of the market, a copying system for making multiplex holographic stereograms, and a special kind of silver halide holographic film developed by us recently. The characteristic of the copying system and the property of the special silver-halide emulsion are introduced in this paper.

  17. Multimegabit Operation Multiplexer System. [PCM telemetry unit for space applications

    NASA Technical Reports Server (NTRS)

    Giri, R. R.; Maxwell, M. S.

    1973-01-01

    The Multimegabit Operation Multiplexer System (MOMS) is a high-data-rate PCM telemetry unit capable of sampling and encoding 60 scanning radiometer and four vidicon channels at 250 kilosamples/second and 5 megasamples/second, respectively. This sampling capacity plus the seven-bit quantization requires a total throughput rate of 40 megasamples/second and 280 megabits/second. To produce these rates efficiently, the system was divided into a pair of identical 140-megabit blocks. A low-power 20-MHz analog multiplexer and analog-to-digital converter were developed together with a video sample-and-hold that features an aperture time error of less than 50 psec. Breadboard testing of these basic building blocks confirmed the design prediction that the total system would consume 27 watts of power. Two 140-megabit output parts are suitable for quadriphase modulation.

  18. Mixed species radioiodine air sampling readout and dose assessment system

    DOEpatents

    Distenfeld, Carl H.; Klemish, Jr., Joseph R.

    1978-01-01

    This invention provides a simple, reliable, inexpensive and portable means and method for determining the thyroid dose rate of mixed airborne species of solid and gaseous radioiodine without requiring highly skilled personnel, such as health physicists or electronics technicians. To this end, this invention provides a means and method for sampling a gas from a source of a mixed species of solid and gaseous radioiodine for collection of the mixed species and readout and assessment of the emissions therefrom by cylindrically, concentrically and annularly molding the respective species around a cylindrical passage for receiving a conventional probe-type Geiger-Mueller radiation detector.

  19. Digital and analog readout systems for fiber-optic strain sensors as applied to the monitoring of roller element bearing systems

    NASA Astrophysics Data System (ADS)

    Juarez, Juan C.; Conkey, Andrew P.; Perez, Robert X.; Taylor, Henry F.

    2003-11-01

    A rotating machinery test rig was instrumented with fiber Fabry-Perot interferometer strain sensors for condition monitoring of rolling element bearings. Strain variations produced by ball passes were observed and analyzed in the time and frequency domain. Wavelength division multiplexing was utilized to simultaneously monitor the sensors with analog and digital readout systems-analog for high bandwidth and digital for high dynamic range and the monitoring of multiple sensors. The effects of imbalance on the shaft, changes in rotational speed, effects on the rotor system, and detection of bearing defects were investigated. Frequency peaks observed in the bearing sensor spectra closely matched predicted values. Imbalance and rotational speed tests showed good agreement with expected trends, and bearing defects were successfully detected.

  20. A 40 GByte/s read-out system for GEM

    SciTech Connect

    Bowden, M.; Carrel, J.; Dorenbosch, J.; Kapoor, V.

    1994-04-01

    The preliminary design of the read-out system for the GEM (Gammas, Electrons, Muons) detector at the Superconducting Super Collider is presented. The system reads all digitized data from the detector data sources at a Level 1 trigger rate of up to 100 kHz. A total read-out bandwidth of 40 GBytes/s is available. Data are stored in buffers that are accessible for further event filtering by an on-line, processor farm. Data are transported to the farm only as they are needed by the higher-level trigger algorithms, leading to a reduced bandwidth requirement in the Data Acquisition System.

  1. An application specific integrated circuit based multi-anode microchannel array readout system

    NASA Technical Reports Server (NTRS)

    Smeins, Larry G.; Stechman, John M.; Cole, Edward H.

    1991-01-01

    Size reduction of two new multi-anode microchannel array (MAMA) readout systems is described. The systems are based on two analog and one digital application specific integrated circuits (ASICs). The new readout systems reduce volume over previous discrete designs by 80 percent while improving electrical performance on virtually every significant parameter. Emphasis is made on the packaging used to achieve the volume reduction. Surface mount technology (SMT) is combined with modular construction for the analog portion of the readout. SMT reliability concerns and the board area impact of MIL SPEC SMT components is addressed. Package selection for the analog ASIC is discussed. Future sytems will require even denser packaging and the volume reduction progression is shown.

  2. Detector apparatus having a hybrid pixel-waveform readout system

    DOEpatents

    Meng, Ling-Jian

    2014-10-21

    A gamma ray detector apparatus comprises a solid state detector that includes a plurality of anode pixels and at least one cathode. The solid state detector is configured for receiving gamma rays during an interaction and inducing a signal in an anode pixel and in a cathode. An anode pixel readout circuit is coupled to the plurality of anode pixels and is configured to read out and process the induced signal in the anode pixel and provide triggering and addressing information. A waveform sampling circuit is coupled to the at least one cathode and configured to read out and process the induced signal in the cathode and determine energy of the interaction, timing of the interaction, and depth of interaction.

  3. A digital readout system for the CMS Phase I Pixel Upgrade

    NASA Astrophysics Data System (ADS)

    Stringer, R.

    2015-04-01

    The Phase I Upgrade to the CMS Pixel Detector at the LHC features a new 400 Mb/s digital readout system. This new system utilizes upgraded custom ASICs, PSI46digv2.1 Read Out Chips and Token Bit Manager for data packaging, new optical links and changes to the Front End Drivers. We are reporting on the new architecture of the full readout chain, the new schema for data encoding/transmission, and the results of preliminary testing of the new optical components.

  4. Conversion factors between human and automatic readouts of CDMAM phantom images of CR mammography systems

    NASA Astrophysics Data System (ADS)

    Figl, Michael; Homolka, Peter; Osanna-Elliott, Angelika; Semturs, Friedrich; Kaar, Marcus; Hummel, Johann

    2016-09-01

    In mammography screening, profound assessment of technical image quality is imperative. The European protocol for the quality control of the physical and technical aspects of mammography screening (EPQCM) suggests using an alternate fixed choice contrast-detail phantom-like CDMAM. For the evaluation of technical image quality, human or automated readouts can be used. For automatic evaluation, a software (cdcom) is provided by EUREF. If the automated readout indicates unacceptable image quality, additional human readout may be performed overriding the automated readout. As the latter systematically results in higher image quality ratings, conversion factors between both methods are regularly applied. Since most image quality issues with mammography systems arise within CR systems, an assessment restricted to CR systems with data from the Austrian Reference Center in the mammography screening program has been conducted. Forty-five CR systems were evaluated. Human readouts were performed with a randomisation software to avoid bias due to learning effects. Additional automatic evaluation allowed for the computation of conversion factors between human and automatic readouts. These factors were substantially lower compared to those suggested by EUREF, namely 1.21 compared to 1.62 (EUREF UK method) and 1.42 (EUREF EU method) for 0.1 mm, and 1.40 compared to 1.83 (EUREF UK) and 1.73 (EUREF EU) for 0.25 mm structure size, respectively. Using either of these factors to adjust patient dose in order to comply with image quality requirements results in differences in the dose increase of up to 90%. This necessitates a consensus on their proper application and limits the validity of the assessment methods. Clear criteria for CR systems based on appropriate studies should be promoted.

  5. Characteristics of a large system of pad readout wire proportional chambers for the HPC calorimeter

    SciTech Connect

    Camporesi, T.; Cavallo, F.R.; Giordano, V.; Laurenti, G.; Molinari, G.; Navarria, F.L.; Privitera, P.; Rovelli, T.; Valenti, G.; Zucchini, A.

    1989-02-01

    A large system of wire proportional chambers is being constructed for the readout of the High-Density Projection Chamber (HPC) of the DELPHI experiment at the Large Electron-Positron storage ring. The system consists of 144 chambers, each 0.3 m/sup 2/ wide and read out via cathode pads, located at the end of the HPC drift volume.

  6. Catch and Release: Integrated system for multiplexed detection of bacteria

    PubMed Central

    Verbarg, Jasenka; Plath, William D.; Shriver-Lake, Lisa C.; Howell, Peter B.; Erickson, Jeffrey S.; Golden, Joel P.; Ligler, Frances S.

    2013-01-01

    An integrated system with automated immunomagnetic separation and processing of fluidic samples was demonstrated for multiplexed optical detection of bacterial targets. Mixtures of target-specific magnetic bead sets were processed in the NRL MagTrap with the aid of rotating magnet arrays that entrapped and moved the beads within the channel during reagent processing. Processing was performed in buffer and human serum matrices with 10-fold dilutions in the range of 102 – 106 cells/mL of target bacteria. Reversal of magnets’ rotation post processing released the beads back into the flow and moved them into the Microflow Cytometer for optical interrogation. Identification of the beads and the detection of PE fluorescence were performed simultaneously for multiplexed detection. Multiplexing was performed with specifically targeted bead sets to detect E. coli 0157.H7, Salmonella Common Structural Antigen, Listeria sp. and Shigella sp. Dose-response curves were obtained, and limits of detection were calculated for each target in the buffer and clinical matrix. Additional tests demonstrated the potential for using the MagTrap to concentrate target from larger volumes of sample prior to the addition of assay reagents. PMID:23631439

  7. Multiplexed logic controls solar-heating system

    NASA Technical Reports Server (NTRS)

    Currie, J. R.

    1981-01-01

    Four inexpensive thermocouples monitor temperatures at key points. On command from logic circuitry, dampers open and close to direct airflow, and fan and auxiliary heater shut on or off. Controlling complex arranges heating system in any one of four operating configurations.

  8. Infrared readout electronics; Proceedings of the Meeting, Orlando, FL, Apr. 21, 22, 1992

    NASA Technical Reports Server (NTRS)

    Fossum, Eric R. (Editor)

    1992-01-01

    The present volume on IR readout electronics discusses cryogenic readout using silicon devices, cryogenic readout using III-V and LTS devices, multiplexers for higher temperatures, and focal-plane signal processing electronics. Attention is given to the optimization of cryogenic CMOS processes for sub-10-K applications, cryogenic measurements of aerojet GaAs n-JFETs, inP-based heterostructure device technology for ultracold readout applications, and a three-terminal semiconductor-superconductor transimpedance amplifier. Topics addressed include unfulfilled needs in IR astronomy focal-plane readout electronics, IR readout integrated circuit technology for tactical missile systems, and radiation-hardened 10-bit A/D for FPA signal processing. Also discussed are the implementation of a noise reduction circuit for spaceflight IR spectrometers, a real-time processor for staring receivers, and a fiber-optic link design for INMOS transputers.

  9. A 128 Multiplexing Factor Time-Domain SQUID Multiplexer

    NASA Astrophysics Data System (ADS)

    Prêle, D.; Voisin, F.; Piat, M.; Decourcelle, T.; Perbost, C.; Chapron, C.; Rambaud, D.; Maestre, S.; Marty, W.; Montier, L.

    2016-07-01

    A cryogenic 128:1 Time-Domain Multiplexer (TDM) has been developed for the readout of kilo-pixel Transition Edge Sensor (TES) arrays dedicated to the Q&U Bolometric Interferometer for Cosmology (QUBIC) instrument which aims to measure the B-mode polarization of the Cosmic Microwave Background. Superconducting QUantum Interference Devices (SQUIDs) are usually used to read out TESs. Moreover, SQUIDs are used to build TDM by biasing sequentially the SQUIDs connected together—one for each TES. In addition to this common technique which allows a typical 32 multiplexing factor, a cryogenic integrated circuit provides a 4:1 second multiplexing stage. This cryogenic integrated circuit is one of the original part of our TDM achieving an unprecedented 128 multiplexing factor. We present these two dimension TDM stages: topology of the SQUID multiplexer, operation of the cryogenic integrated circuit, and integration of the full system to read out a TES array dedicated to the QUBIC instrument. Flux-locked loop operation in multiplexed mode is also discussed.

  10. A feasibility study of a data acquisition system for a silicon strip detector with a digital readout scheme

    NASA Astrophysics Data System (ADS)

    Ikeda, Hirokazu; Ikeda, Mitsuo; Inaba, Susumu; Tanaka, Manobu

    1993-06-01

    We describe a prototype data acquisition system for a silicon strip detector, which has been developed in terms of a digital readout scheme. The system consists of a master timing generator, readout controller, and a detector emulator card on which we use custom VLSI shift registers with operating clock frequency of 30 MHz.

  11. Readout Circuit System for In2O3/RGO Nanocomposite Gas Sensors

    NASA Astrophysics Data System (ADS)

    Lin, Cheng-Yi

    A readout circuit system for In2O3/RGO nanocomposite gas sensors using open-source software has been developed for the first time. The readout system adopts a Raspberry Pi as an electronic control unit and incorporates different electronics components to realize the function of a source measure unit (SMU). During the operation, real-time results of measured gas concentrations can be accessed through the Internet and alarm functions are also included. All control programs were written in Python language. Using this readout system, current response of gas sensors toward oxygen concentrations (2,000---32,000 ppm) in argon environment at 140 °C are in a good agreement with the data measured by Agilent SMU (B2902A). Furthermore, temperature effects and transient response of the proposed system are investigated. The success of this readout system demonstrates the potential use of open-source hardware to construct scientific instruments with the advantages of miniaturization, low cost, flexible design, and Internet access.

  12. Multiplex electric discharge gas laser system

    NASA Technical Reports Server (NTRS)

    Laudenslager, James B. (Inventor); Pacala, Thomas J. (Inventor)

    1987-01-01

    A multiple pulse electric discharge gas laser system is described in which a plurality of pulsed electric discharge gas lasers are supported in a common housing. Each laser is supplied with excitation pulses from a separate power supply. A controller, which may be a microprocessor, is connected to each power supply for controlling the application of excitation pulses to each laser so that the lasers can be fired simultaneously or in any desired sequence. The output light beams from the individual lasers may be combined or utilized independently, depending on the desired application. The individual lasers may include multiple pairs of discharge electrodes with a separate power supply connected across each electrode pair so that multiple light output beams can be generated from a single laser tube and combined or utilized separately.

  13. Development of microwave kinetic inductance detectors and their readout system for LiteBIRD

    NASA Astrophysics Data System (ADS)

    Hattori, K.; Hazumi, M.; Ishino, H.; Kibayashi, A.; Kibe, Y.; Mima, S.; Okamura, T.; Sato, N.; Tomaru, T.; Yamada, Y.; Yoshida, M.; Yuasa, T.; Watanabe, H.

    2013-12-01

    Primordial gravitational waves generated by inflation have produced an odd-parity pattern B-mode in the cosmic microwave background (CMB) polarization. LiteBIRD (Light satellite for the studies of B-mode polarization and Inflation from cosmic background Radiation Detection) aims at detecting this B-mode polarization precisely. It requires about 2000 detectors capable of detecting a frequency range from 50 GHz to 250 GHz with ultra low noise. Superconducting detectors are suitable for this requirement. We have fabricated and tested microwave kinetic inductance detectors (MKIDs) and developed a new readout system. We have designed antenna-coupled MKIDs. Quasi-particles are created by incident radiation and are detected as a change of the surface impedance of a superconductor strip. This change of the surface impedance is translated into the change of the resonant frequency of a microwave signal transmitted through the resonator. We also have developed a new readout system for MKIDs. The newly developed readout system is not only able to read out the amplitude and the phase data with the homodyne detection for multi-channels, but also provides a unique feature of tracking the resonant frequency of the target resonator. This mechanism enables us to detect signals with a large dynamic range. We report on the recent R&D status of the developing MKIDs and on the read-out system for LiteBIRD.

  14. A fast embedded readout system for large-area Medipix and Timepix systems

    NASA Astrophysics Data System (ADS)

    Brogna, A. S.; Balzer, M.; Smale, S.; Hartmann, J.; Bormann, D.; Hamann, E.; Cecilia, A.; Zuber, M.; Koenig, T.; Zwerger, A.; Weber, M.; Fiederle, M.; Baumbach, T.

    2014-05-01

    In this work we present a novel readout electronics for an X-ray sensor based on a Si crystal bump-bonded to an array of 3 × 2 Medipix ASICs. The pixel size is 55 μm × 55 μm with a total number of ~ 400k pixels and a sensitive area of 42 mm × 28 mm. The readout electronics operate Medipix-2 MXR or Timepix ASICs with a clock speed of 125 MHz. The data acquisition system is centered around an FPGA and each of the six ASICs has a dedicated I/O port for simultaneous data acquisition. The settings of the auxiliary devices (ADCs and DACs) are also processed in the FPGA. Moreover, a high-resolution timer operates the electronic shutter to select the exposure time from 8 ns to several milliseconds. A sophisticated trigger is available in hardware and software to synchronize the acquisition with external electro-mechanical motors. The system includes a diagnostic subsystem to check the sensor temperature and to control the cooling Peltier cells and a programmable high-voltage generator to bias the crystal. A network cable transfers the data, encapsulated into the UDP protocol and streamed at 1 Gb/s. Therefore most notebooks or personal computers are able to process the data and to program the system without a dedicated interface. The data readout software is compatible with the well-known Pixelman 2.x running both on Windows and GNU/Linux. Furthermore the open architecture encourages users to write their own applications. With a low-level interface library which implements all the basic features, a MATLAB or Python script can be implemented for special manipulations of the raw data. In this paper we present selected images taken with a microfocus X-ray tube to demonstrate the capability to collect the data at rates up to 120 fps corresponding to 0.76 Gb/s.

  15. Development of readout system for FE-I4 pixel module using SiTCP

    NASA Astrophysics Data System (ADS)

    Teoh, J. J.; Hanagaki, K.; Ikegami, Y.; Takubo, Y.; Terada, S.; Unno, Y.

    2013-12-01

    The ATLAS pixel detector will be replaced in the future High Luminosity-Large Hadron Collider (HL-LHC) upgrade to preserve or improve the detector performance at high luminosity environment. To meet the tight requirements of the upgrade, a new pixel Front-End (FE) Integrated Circuit (IC) called FE-I4 has been developed. We have then devised a readout system for the new FE IC. Our system incorporates Silicon Transmission Control Protocol (SiTCP) technology (Uchida, 2008 [1]) which utilizes the standard TCP/IP and UDP communication protocols. This technology allows direct data access and transfer between a readout hardware chain and PC via a high speed Ethernet. In addition, the communication protocols are small enough to be implemented in a single Field-Programable Gate Array (FPGA). Relying on this technology, we have been able to construct a very compact, versatile and fast readout system. We have developed a firmware and software together with the readout hardware chain. We also have established basic functionalities for reading out FE-I4.

  16. Capillaries for use in a multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, E.S.; Chang, H.T.; Fung, E.N.

    1997-12-09

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

  17. Capillaries for use in a multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, Edward S.; Chang, Huan-Tsang; Fung, Eliza N.

    1997-12-09

    The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

  18. Development of a multiplexed bypass control system for aerospace batteries

    NASA Technical Reports Server (NTRS)

    Frank, H. A.

    1977-01-01

    A breadboard bypass control system was developed to control a battery comprised of 26 JPL-developed negative limited Ni-Cd cells. The system was designed to automatically remove cells from the circuit when their voltages exceeded a fixed limit on charge and fell below a fixed limit on discharge. Major components of the system consisted of a cell voltage monitor, a multiplexing circuit, and individual electromechanical relays for each cell. The system was found to function well in controlling the battery during a simulated 10-month MM-71 mission and a 2-month simulated low earth orbit cycling mission. A flight version of the bypass system was estimated to have a total parts count of 150 and total weight of 1.63 kg. When fully developed, the system shows promise for improving life and reliability of spacecraft batteries.

  19. A new ATLAS muon CSC readout system with system on chip technology on ATCA platform

    NASA Astrophysics Data System (ADS)

    Claus, R.

    2016-07-01

    The ATLAS muon Cathode Strip Chamber (CSC) back-end readout system has been upgraded during the LHC 2013-2015 shutdown to be able to handle the higher Level-1 trigger rate of 100 kHz and the higher occupancy at Run 2 luminosity. The readout design is based on the Reconfiguration Cluster Element (RCE) concept for high bandwidth generic DAQ implemented on the ATCA platform. The RCE design is based on the new System on Chip Xilinx Zynq series with a processor-centric architecture with ARM processor embedded in FPGA fabric and high speed I/O resources together with auxiliary memories to form a versatile DAQ building block that can host applications tapping into both software and firmware resources. The Cluster on Board (COB) ATCA carrier hosts RCE mezzanines and an embedded Fulcrum network switch to form an online DAQ processing cluster. More compact firmware solutions on the Zynq for G-link, S-link and TTC allowed the full system of 320 G-links from the 32 chambers to be processed by 6 COBs in one ATCA shelf through software waveform feature extraction to output 32 S-links. The full system was installed in Sept. 2014. We will present the RCE/COB design concept, the firmware and software processing architecture, and the experience from the intense commissioning towards LHC Run 2.

  20. A new ATLAS muon CSC readout system with system on chip technology on ATCA platform

    DOE PAGESBeta

    Claus, R.

    2015-10-23

    The ATLAS muon Cathode Strip Chamber (CSC) back-end readout system has been upgraded during the LHC 2013-2015 shutdown to be able to handle the higher Level-1 trigger rate of 100 kHz and the higher occupancy at Run 2 luminosity. The readout design is based on the Reconfiguration Cluster Element (RCE) concept for high bandwidth generic DAQ implemented on the ATCA platform. The RCE design is based on the new System on Chip Xilinx Zynq series with a processor-centric architecture with ARM processor embedded in FPGA fabric and high speed I/O resources together with auxiliary memories to form a versatile DAQmore » building block that can host applications tapping into both software and firmware resources. The Cluster on Board (COB) ATCA carrier hosts RCE mezzanines and an embedded Fulcrum network switch to form an online DAQ processing cluster. More compact firmware solutions on the Zynq for G-link, S-link and TTC allowed the full system of 320 G-links from the 32 chambers to be processed by 6 COBs in one ATCA shelf through software waveform feature extraction to output 32 S-links. Furthermore, the full system was installed in Sept. 2014. We will present the RCE/COB design concept, the firmware and software processing architecture, and the experience from the intense commissioning towards LHC Run 2.« less

  1. A new ATLAS muon CSC readout system with system on chip technology on ATCA platform

    SciTech Connect

    Claus, R.

    2015-10-23

    The ATLAS muon Cathode Strip Chamber (CSC) back-end readout system has been upgraded during the LHC 2013-2015 shutdown to be able to handle the higher Level-1 trigger rate of 100 kHz and the higher occupancy at Run 2 luminosity. The readout design is based on the Reconfiguration Cluster Element (RCE) concept for high bandwidth generic DAQ implemented on the ATCA platform. The RCE design is based on the new System on Chip Xilinx Zynq series with a processor-centric architecture with ARM processor embedded in FPGA fabric and high speed I/O resources together with auxiliary memories to form a versatile DAQ building block that can host applications tapping into both software and firmware resources. The Cluster on Board (COB) ATCA carrier hosts RCE mezzanines and an embedded Fulcrum network switch to form an online DAQ processing cluster. More compact firmware solutions on the Zynq for G-link, S-link and TTC allowed the full system of 320 G-links from the 32 chambers to be processed by 6 COBs in one ATCA shelf through software waveform feature extraction to output 32 S-links. Furthermore, the full system was installed in Sept. 2014. We will present the RCE/COB design concept, the firmware and software processing architecture, and the experience from the intense commissioning towards LHC Run 2.

  2. A new ATLAS muon CSC readout system with system on chip technology on ATCA platform

    NASA Astrophysics Data System (ADS)

    Bartoldus, R.; Claus, R.; Garelli, N.; Herbst, R. T.; Huffer, M.; Iakovidis, G.; Iordanidou, K.; Kwan, K.; Kocian, M.; Lankford, A. J.; Moschovakos, P.; Nelson, A.; Ntekas, K.; Ruckman, L.; Russell, J.; Schernau, M.; Schlenker, S.; Su, D.; Valderanis, C.; Wittgen, M.; Yildiz, S. C.

    2016-01-01

    The ATLAS muon Cathode Strip Chamber (CSC) backend readout system has been upgraded during the LHC 2013-2015 shutdown to be able to handle the higher Level-1 trigger rate of 100 kHz and the higher occupancy at Run-2 luminosity. The readout design is based on the Reconfigurable Cluster Element (RCE) concept for high bandwidth generic DAQ implemented on the Advanced Telecommunication Computing Architecture (ATCA) platform. The RCE design is based on the new System on Chip XILINX ZYNQ series with a processor-centric architecture with ARM processor embedded in FPGA fabric and high speed I/O resources. Together with auxiliary memories, all these components form a versatile DAQ building block that can host applications tapping into both software and firmware resources. The Cluster on Board (COB) ATCA carrier hosts RCE mezzanines and an embedded Fulcrum network switch to form an online DAQ processing cluster. More compact firmware solutions on the ZYNQ for high speed input and output fiberoptic links and TTC allowed the full system of 320 input links from the 32 chambers to be processed by 6 COBs in one ATCA shelf. The full system was installed in September 2014. We will present the RCE/COB design concept, the firmware and software processing architecture, and the experience from the intense commissioning for LHC Run 2.

  3. A new ATLAS muon CSC readout system with system on chip technology on ATCA platform

    DOE PAGESBeta

    Bartoldus, R.; Claus, R.; Garelli, N.; Herbst, R. T.; Huffer, M.; Iakovidis, G.; Iordanidou, K.; Kwan, K.; Kocian, M.; Lankford, A. J.; et al

    2016-01-25

    The ATLAS muon Cathode Strip Chamber (CSC) backend readout system has been upgraded during the LHC 2013-2015 shutdown to be able to handle the higher Level-1 trigger rate of 100 kHz and the higher occupancy at Run-2 luminosity. The readout design is based on the Reconfigurable Cluster Element (RCE) concept for high bandwidth generic DAQ implemented on the Advanced Telecommunication Computing Architecture (ATCA) platform. The RCE design is based on the new System on Chip XILINX ZYNQ series with a processor-centric architecture with ARM processor embedded in FPGA fabric and high speed I/O resources. Together with auxiliary memories, all ofmore » these components form a versatile DAQ building block that can host applications tapping into both software and firmware resources. The Cluster on Board (COB) ATCA carrier hosts RCE mezzanines and an embedded Fulcrum network switch to form an online DAQ processing cluster. More compact firmware solutions on the ZYNQ for high speed input and output fiberoptic links and TTC allowed the full system of 320 input links from the 32 chambers to be processed by 6 COBs in one ATCA shelf. The full system was installed in September 2014. In conclusion, we will present the RCE/COB design concept, the firmware and software processing architecture, and the experience from the intense commissioning for LHC Run 2.« less

  4. Multiplex microfluidic system integrating sequential operations of microalgal lipid production.

    PubMed

    Kwak, Ho Seok; Kim, Jaoon Young Hwan; Na, Sang Cheol; Jeon, Noo Li; Sim, Sang Jun

    2016-02-21

    The unit cost for the production of algal biofuel needs to be reduced in order to be a substitute for fossil fuel. To achieve this goal, the development of a novel system is needed for a rapid screening of numerous microalgal species to isolate superior strains with the highest lipid productivity. Here, we developed a PDMS-based multiplex microfluidic system with eight chambers and micropillar arrays to expedite multiple steps for lipid sample preparation from different microalgal strains. We could rapidly and efficiently perform sequential operations from cell culture to lipid extraction of eight different microalgal strains simultaneously on a single device without harvesting and purification steps, which are labor- and energy-intensive, by the simple injection of medium and solvent into the central inlet due to the integrated micropillar arrays connecting the chambers and central inlet. The lipid extraction efficiency using this system was comparable (94.5-102.6%) to the conventional Bligh-Dyer method. We investigated the cell growth and lipid productivity of different strains using the microfluidic device. We observed that each strain has a different lipid accumulation pattern according to stress conditions. These results demonstrate that our multiplex microfluidic approach can provide an efficient analytical tool for the rapid analysis of strain performances (e.g. cell growth and lipid productivities) and the determination of the optimal lipid induction condition for each strain. PMID:26783562

  5. Giant magnetoresistive-based biosensing probe station system for multiplex protein assays.

    PubMed

    Wang, Yi; Wang, Wei; Yu, Lina; Tu, Liang; Feng, Yinglong; Klein, Todd; Wang, Jian-Ping

    2015-08-15

    In this study, a sensitive immune-biosensing system capable of multiplexed, real-time electrical readout was developed based on giant magnetoresistive (GMR) sensor array to detect a panel of protein biomarkers simultaneously. PAPP-A, PCSK9, and ST2 have been regarded as promising candidate biomarkers for cardiovascular diseases. Early detection of multiple biomarkers for a disease could enable accurate prediction of a disease risk. 64 nano-size GMR sensors were assembled onto one 16 mm × 16 mm chip with a reaction well, and they could work independently and be monitored simultaneously. A detect limit of 40 pg/mL for ST2 antigen had been achieved, and the dynamic ranges for the three proteins detection were up to four orders of magnitude. The GMR sensing platform was also selective enough to be directly used in serum samples. In addition, a lab-based probe station has been designed to implement quick lab-on-a-chip experiments instead of wire bonding. It has a potential application in clinical biomarkers identification and screening, and can be extended to fit other biosensing schemes. PMID:25794959

  6. Side illuminated optical fiber as a multiplexing element for spectroscopic systems

    NASA Astrophysics Data System (ADS)

    Egalon, Claudio O.; Matta, Michael P.; Lavezzari, Delbert C.; Insley, Robert Y.; Jaring, Carolyn C.; Quiday, Marie F.

    2013-09-01

    A new type of colorimeter with multiple channels was demonstrated using a side illuminated optical fiber. When different spots of a properly modified fiber are side illuminated, multiple signals are generated and guided by the waveguide: the essence of multiplexing. This configuration is simple, low cost, does not require a sensitive coating and can analyze several samples along the fiber with a single detector: the most expensive component. Since regular colorimeters use one detector per sample, our new configuration considerably lowers the cost of analyzing multiple samples. This system consists of a fiber mounted over a support, three LEDs, an LED driver, a photo diode and a read-out: to increase the signal, the fiber was tapered. For calibration purposes, six solutions of different concentrations of food dye were prepared, placed inside cuvettes along the fiber length and illuminated by the LEDs. This light passes through the solution, strikes the fiber and is guided to the detector: the darker the solution the lower the signal intensity. Several calibration curves were obtained using different light intensities: it was found that the greater the intensity, the higher the colorimeter sensitivity. This simple capability can be used to easily control the device's sensitivity and its resolution. Although built for three samples only, this device can be modified to accommodate more. With cuvettes measuring 1 cm, it is possible to accommodate one sample per cm of fiber. Also, with minor modifications, this colorimeter can be used for fluorescence, scattering and index of refraction measurements.

  7. Development of a fast readout system of an X-ray CCD

    NASA Astrophysics Data System (ADS)

    Awaki, H.; Hamaguchi, K.; Koyama, K.; Tomida, H.; Tsuru, T.

    1999-10-01

    In the course of developing a fast readout system of an X-ray CCD camera with low noise using HPK-CCD, we have succeeded in making a clock generator to read data from a CCD with the speed of 2 Mpixel/s. This generator is fabricated from commercially available products in order to simplify the manufacturing procedure. To determine the noise of the system, we are evaluating the noise from each part which conceivably contributes. It is known that the readout noise depends on the clock speed. Thus, to begin with, we measured the dependency of the system noise from the ADC in the data acquisition system on its clock speed.

  8. Wavelength readout system constructed of fiber Fabry-Perot tunable filter and virtual instrument

    NASA Astrophysics Data System (ADS)

    Lü, Chengang; Zhang, Ruifeng; Cheng, Pengfei; Li, Kejia; Wu, Xing; Ge, Chunfeng

    2010-08-01

    The wavelength readout system reported in this paper is chiefly constructed of fiber Fabry-Perot tunable filter (FFP-TF), data acquisition card and a virtual instrument with programmable NI Labview. By combining the dynamic scanning of FFP-TF in C waveband (1520nm-1570nm) with 50nm free spectral range (FSR) and 4000 standard finesse value, the wavelength of a tested laser diode (LD) could be detected accurately, while the spectrum is displayed on line with the help of a virtual instrument to make the spectroscopy quick analysis possible. Furthermore, the scheme can also be applied for wavelength interrogation in fiber Bragg grating (FBG) sensing system. Considering the practibility and economical efficiency of such a system, it will be of great significance to adopt such a wavelength readout system in fiber sensors used for construction, mining, aerospace,etc.

  9. Selection of chromosomal DNA libraries using a multiplex CRISPR system.

    PubMed

    Ryan, Owen W; Skerker, Jeffrey M; Maurer, Matthew J; Li, Xin; Tsai, Jordan C; Poddar, Snigdha; Lee, Michael E; DeLoache, Will; Dueber, John E; Arkin, Adam P; Cate, Jamie H D

    2014-08-19

    The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the use of complex marker-dependent chromosomal integration strategies. We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae genome by optimizing an efficient single-step and marker-free genome editing system using CRISPR-Cas9. With this Multiplex CRISPR (CRISPRm) system, we selected an improved cellobiose utilization pathway in diploid yeast in a single round of mutagenesis and selection, which increased cellobiose fermentation rates by over 10-fold. Mutations recovered in the best cellodextrin transporters reveal synergy between substrate binding and transporter dynamics, and demonstrate the power of CRISPRm to accelerate selection experiments and discoveries of the molecular determinants that enhance biomolecule function.

  10. A new TDR multiplexing system for reliable electrical conductivity and soil water content measurements

    NASA Astrophysics Data System (ADS)

    Weihermueller, Lutz; Huisman, Sander; Hermes, Normen; Pickel, Stefanie; Vereecken, Harry

    2013-04-01

    Time domain reflectometry (TDR) is a standard method to estimate soil water content and bulk soil electrical conductivity. In many applications, several TDR probes are installed in soil columns or field setups, and they are measured using a multiplexing system. It has been reported that commercially available multiplexers share a common ground, which might lead to inaccurate TDR measurements when probes are installed close together or at sites with high electromagnetic noise. Therefore, a new eight-channel differential multiplexer (50C81-SDM) was developed that allows communication with standard TDR equipment. The 50C81-SDM multiplexer was tested using measurement in electrolyte solutions and a sand tank. In contrast to multiplexers with a common ground, they showed no interference of closely spaced TDR probes. Measurements at a test site also showed the applicability of the 50C81-SDM multiplexer in an environment contaminated with high electromagnetic noise.

  11. Development and population study of an eight-locus short tandem repeat (STR) multiplex system.

    PubMed

    Lins, A M; Micka, K A; Sprecher, C J; Taylor, J A; Bacher, J W; Rabbach, D R; Bever, R A; Creacy, S D; Schumm, J W

    1998-11-01

    Amplification of short tandem repeat (STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for such uses, the polymorphic STR loci CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D5S818, F13A01, FESFPS, F13B, and LPL have been evaluated, developed, and configured into fluorescently labeled multiplex systems. Eight of these STR loci were combined to generate the PowerPlex System, a two-color multiplex system that supports rapid, accurate, reliable analysis and designation of alleles. The remaining four loci comprise the FFFL System, a one-color multiplex system. The PowerPlex System may be evaluated alternatively as two one-color, four-locus multiplex systems, CTTv Multiplex and GammaSTR Multiplex. The products of multiplex amplification may be analyzed with a variety of fluorescence detection instruments. Determination of genotypes of over 200 individuals from each of three different population/ethnic groups revealed independence of inheritance of the loci and allowed calculation of matching probability, typical paternity index, and power of exclusion for each multiplex.

  12. Wide angle holographic display system with spatiotemporal multiplexing.

    PubMed

    Kozacki, Tomasz; Finke, Grzegorz; Garbat, Piotr; Zaperty, Weronika; Kujawińska, Małgorzata

    2012-12-01

    This paper presents a wide angle holographic display system with extended viewing angle in both horizontal and vertical directions. The display is constructed from six spatial light modulators (SLM) arranged on a circle and an additional SLM used for spatiotemporal multiplexing and a viewing angle extension in two perpendicular directions. The additional SLM, that is synchronized with the SLMs on the circle is placed in the image space. This method increases effective space bandwidth product of display system data from 12.4 to 50 megapixels. The software solution based on three Nvidia graphic cards is developed and implemented in order to achieve fast and synchronized displaying. The experiments presented for both synthetic and real 3D data prove the possibility to view binocularly having good quality images reconstructed in full FoV of the display. PMID:23262697

  13. A prototype scalable readout system for micro-pattern gas detectors

    NASA Astrophysics Data System (ADS)

    Zheng, Qi-Bin; Liu, Shu-Bin; Tian, Jing; Li, Cheng; Feng, Chang-Qing; An, Qi

    2016-08-01

    A scalable readout system (SRS) is designed to provide a general solution for different micro-pattern gas detectors in various applications. The system mainly consists of three kinds of modules: the ASIC card, the adapter card and the front-end card (FEC). The ASIC cards, mounted with particular ASIC chips, are designed for receiving detector signals. The adapter card is in charge of digitizing the output signals from several ASIC cards. The FEC, edged-mounted with the adapter, has field-programmable gate array (FPGA)-based reconfigurable logic and I/O interfaces, allowing users to choose different ASIC cards and adapters for different experiments, which expands the system to various applications. The FEC transfers data through Gigabit Ethernet protocol realized by a TCP processor (SiTCP) IP core in FPGA. By assembling a flexible number of FECs in parallel through Gigabit Ethernet, the readout system can be tailored to specific sizes to adapt to the experiment scales and readout requirements. In this paper, two kinds of multi-channel ASIC chip, VA140 and AGET, are applied to verify the scalability of this SRS architecture. Based on this VA140 or AGET SRS, one FEC covers 8 ASIC (VA140) cards handling 512 detector channels, or 4 ASIC (AGET) cards handling 256 detector channels, respectively. More FECs can be assembled in crates to handle thousands of detector channels. Supported by National Natural Science Foundation of China (11222552)

  14. Front-end readout electronics considerations for Silicon Tracking System and Muon Chamber

    NASA Astrophysics Data System (ADS)

    Kasinski, K.; Kleczek, R.; Szczygiel, R.

    2016-02-01

    Silicon Tracking System (STS) and Muon Chamber (MUCH) are components of the Compressed Baryonic Matter (CBM) experiment at FAIR, Germany. STS will be built from 8 detector stations located in the aperture of the magnet. Each station will be built from double-sided silicon strip detectors and connected via kapton microcables to the readout electronics at the perimeter of each station. The challenging physics program of the CBM experiment requires from the detector systems very high performance. Design of the readout ASIC requires finding an optimal solution for interaction time and input charge measurements in the presence of: tight area (channel pitch: 58 μ m), noise (< 1000 e- rms), power (< 10 mW/channel), radiation hardness and speed requirements (average hit rate: 250 khit/s/channel). This paper presents the front-end electronics' analysis towards prototype STS and MUCH readout ASIC implementation in the UMC 180 nm CMOS process and in-system performance with the emphasis on preferable detector and kapton microcable parameters and input amplifiers' architecture and design.

  15. DBS -- An rlogin multiplexer and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-04-01

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system -- the DA for the 96` round of experiments at Fermilab -- though it has potential usefulness as a powerful tool in other distributed applications. dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) log files (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session -- dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  16. Performance of Front-End Readout System for PHENIX RICH

    SciTech Connect

    Oyama, K.; Hamagaki, H.; Nishimura, S.; Shigaki, K.; Hayano, R.S.; Hibino, M.; Kametani, S.; Kikuchi, J.; Matsumoto, T.; Sakaguchi, T.; Ebisu, K.; Hara, H.; Tanaka, Y.; Ushiroda, T.; Moscone, C.G.; Wintenberg, A.L.; Young, G.R.

    1999-11-15

    A front-end electronics system has been developed for the Ring Imaging Cerenkov (RICH) detector of the PHENIX experiment at the Relativistic Heavy Ion Collider (RHIC), Brookhaven National Laboratory (BNL). A high speed custom back-plane with source synchronous bus architecture, a full custom analog ASIC, and board modules with FPGA's and CPLD's were developed for high performance real time data acquisition. The transfer rate of the back-lane has reached 640 MB/s with 128 bits data bus. Total transaction time is estimated to be less than 30 {micro}s per event. The design specifications and test results of the system are presented in this paper.

  17. System Architecture of the Dark Energy Survey Camera Readout Electronics

    SciTech Connect

    Shaw, Theresa; Ballester, Otger; Cardiel-Sas, Laia; Castilla, Javier; Chappa, Steve; de Vicente, Juan; Holm, Scott; Huffman, Dave; Kozlovsky, Mark; Martinez, Gustavo; Moore, Todd; /Madrid, CIEMAT /Fermilab /Illinois U., Urbana /Fermilab

    2010-05-27

    The Dark Energy Survey makes use of a new camera, the Dark Energy Camera (DECam). DECam will be installed in the Blanco 4M telescope at Cerro Tololo Inter-American Observatory (CTIO). DECam is presently under construction and is expected to be ready for observations in the fall of 2011. The focal plane will make use of 62 2Kx4K and 12 2kx2k fully depleted Charge-Coupled Devices (CCDs) for guiding, alignment and focus. This paper will describe design considerations of the system; including, the entire signal path used to read out the CCDs, the development of a custom crate and backplane, the overall grounding scheme and early results of system tests.

  18. A 1152 channel timing system for an electromagnetic calorimeter readout

    NASA Astrophysics Data System (ADS)

    Bonesini, M.; Bonvin, E.; Booth, P. S. L.; Carroll, L. J.; Cass, A. J.; Cavalli, D.; Cecchet, G.; Costa, G.; Donnat, M.; Dorsaz, P. A.; Edwards, D. N.; Fischer, J. R.; Fluri, L.; Frame, D.; Gianotti, F.; Jack, S.; Jackson, J. N.; Kelly, M.; Kienzle-Focacci, M. N.; Lucock, R.; Lynch, J. G.; Mandelli, L.; Martin, M.; Mathys, L.; Maxwell, A.; Mazzanti, M.; Myerscough, J. J.; Negus, P. J.; Pensotti-Rancoita, S.; Perini, L.; Perrin, D.; Range, W. H.; Rosselet, L.; Rutschmann, J.; Snow, S. W.; Tamborini, M.; Thompson, A. S.; Turnbull, R. M.; Wells, J.; Werlen, M.

    1988-01-01

    A 1152 channel timing system used with a large electromagnetic calorimeter is described. Analysis of the timing information from the vertical and horizontal elements of the calorimeter yielded values for the position coordinates of the showers. This information was used to resolve ambiguities in the pattern recognition arising from multiple showers. A resolution of better than 0.25 ns for all channels was achieved. The calibration methods employed to maintain this resolution over several years are discussed.

  19. Daisy chain expandable readout system for infrared arrays

    NASA Astrophysics Data System (ADS)

    Nagayama, Takahiro; Haraguchi, Kentaro

    2008-07-01

    We have developed a control system for infrared array detectors with 16, 32 or more outputs. Our system consists of five boards (clock pattern generator, clock driver, A/D converter, parallel-in, and isolation), and is operated with a Linux (kernel 2.4 or 2.6) PC. It is capable of supplying 24 DC bias voltages and 16 clock voltages, adjustable between -7.5V and +7.5V and the shortest clock width of 156 ns. One A/D board converts 16 analog array outputs to digital data simultaneously using 16 A/D converters. The rms of A/D conversions for fixed voltages is 2-3 ADU (or 150-200 μV) at a sampling rate of 250 kSPS. The parallel-in board has 32 optically isolated input channels, and can receive data from 2 A/D boards simultaneously. The maximum data rate to main memory of PC is 40 MB/sec, corresponding to 20 frames/sec of a 1024×1024 array. Our system is now utilized for Aladdin 2 (InSb, 1024×1024, 32 outputs) of Wide Field Cryogenic Telescope 2 and SB-774 (Si:As, 320×240, 16 outputs) of 17μm Fabry-Perot spectrometer. The A/D boards have daisy-chain capability for next generation arrays with more than 32 outputs. In the daisy-chain mode, all A/D converters are triggered simultaneously, but the A/D boards make time-delayed data transfer. The parallel-in board receives data sequentially by every 32 A/D converters. When we apply our system for 2048×2048 detectors with 64 outputs, the frame rate will be 5 frames/sec.

  20. The Detector and Readout Systems of the Micro-X High Resolution Microcalorimeter X-Ray Imaging Rocket

    NASA Astrophysics Data System (ADS)

    Wikus, P.; Doriese, W. B.; Eckart, M. E.; Adams, J. S.; Bandler, S. R.; Brekosky, R. P.; Chervenak, J. A.; Ewin, A. J.; Figueroa-Feliciano, E.; Finkbeiner, F. M.; Galeazzi, M.; Hilton, G.; Irwin, K. D.; Kelley, R. L.; Kilbourne, C. A.; Leman, S. W.; McCammon, D.; Porter, F. S.; Reintsema, C. D.; Rutherford, J. M.; Trowbridge, S. N.

    2009-12-01

    The Micro-X sounding rocket experiment will deploy an imaging transition-edge-sensor (TES) microcalorimeter spectrometer to observe astrophysical sources in the 0.2-3.0 keV band. The instrument has been designed at a systems level, and the first items of flight hardware are presently being built. In the first flight, planned for January 2011, the spectrometer will observe a recently discovered Silicon knot in the Puppis-A supernova remnant. Here we describe the design of the Micro-X science instrument, focusing on the instrument's detector and detector assembly. The current design of the 2-dimensional spectrometer array contains 128 close-packed pixels with a pitch of 600 μm. The conically approximated Wolter-1 mirror will map each of these pixels to a 0.95 arcmin region on the sky; the field of view will be 11.4 arcmin. Targeted energy resolution of the TESs is about 2 eV over the full observing band. A SQUID time-division multiplexer (TDM) will read out the array. The detector time constants will be engineered to approximately 2 ms to match the TDM, which samples each pixel at 32.6 kHz, limited only by the telemetry system of the rocket. The detector array and two SQUID stages of the TDM readout system are accommodated in a lightweight Mg enclosure, which is mounted to the 50 mK stage of an adiabatic demagnetization refrigerator. A third SQUID amplification stage is located on the 1.6 K liquid He stage of the cryostat. An on-board 55-Fe source will fluoresce a Ca target, providing 3.69 and 4.01 keV calibration lines that will not interfere with the scientifically interesting energy band.

  1. Self-triggering readout system for the neutron lifetime experiment PENeLOPE

    NASA Astrophysics Data System (ADS)

    Gaisbauer, D.; Bai, Y.; Konorov, I.; Paul, S.; Steffen, D.

    2016-02-01

    PENeLOPE is a neutron lifetime measurement developed at the Technische Universität München and located at the Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRM II) aiming to achieve a precision of 0.1 seconds. The detector for PENeLOPE consists of about 1250 Avalanche Photodiodes (APDs) with a total active area of 1225 cm2. The decay proton detector and electronics will be operated at a high electrostatic potential of -30 kV and a magnetic field of 0.6 T. This includes shaper, preamplifier, ADC and FPGA cards. In addition, the APDs will be cooled to 77 K. The 1250 APDs are divided into 14 groups of 96 channels, including spares. A 12-bit ADC digitizes the detector signals with 1 MSps. A firmware was developed for the detector including a self-triggering readout with continuous pedestal calculation and configurable signal detection. The data transmission and configuration is done via the Switched Enabling Protocol (SEP). It is a time-division multiplexing low layer protocol which provides determined latency for time critical messages, IPBus, and JTAG interfaces. The network has a n:1 topology, reducing the number of optical links.

  2. Evaluation of White Light Sources For an Absolute Fiber Optic Sensor Readout System

    SciTech Connect

    McConaghy, C F

    2003-10-10

    This report summarizes work done in pursuit of an absolute readout system for Fabry-Perot optics sensors such as those built both by FISO and LLNL. The use of white light results in a short coherence length reducing the ambiguity of the Fabry-Perot gap measurement which is required to readout the sensor. The light source coherence length is the critical parameter in determining the ability to build a relative or an absolute system. Optical sources such as lasers and LEDs are rather narrow in optical spectral bandwidth and have long coherence length. Thus, when used in interferometric sensor measurements, one fringe looks much like another and it is difficult to make an absolute measurement. In contrast, white light sources are much broader in spectral bandwidth and have very short coherence lengths making interferometry possible only over the coherence length, which can be 1 or 2 microns. The small number of fringes in the interferogram make it easier to calculate the centroid and to unambiguously determine the sensor gap. However, unlike LEDs and Lasers, white light sources have very low optical power when coupled into optical fibers. Although, the overall light output of a white light source can be hundreds of milliwatts to watts, it is difficult to couple more than microwatts into a 50-micron core optical fiber. In addition, white light sources have a large amount of optical power in spectrum that is not necessarily useful in terms of sensor measurements. The reflectivity of a quarter wave of Titanium Oxide is depicted in Figure 2. This coating of Titanium Oxide is used in the fabrication of the sensor. This figure shows that any light emitted at wavelengths shorter than 600 nm is not too useful for the readout system. A white light LED spectrum is depicted in Figure 3 and shows much of the spectrum below 600 nm. In addition Silicon photodiodes are usually used in the readout system limiting the longest wavelength to about 1100 nm. Tungsten filament sources may

  3. Interlaboratory study of DNA extraction from multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR for individual kernel detection system of genetically modified maize.

    PubMed

    Akiyama, Hiroshi; Sakata, Kozue; Makiyma, Daiki; Nakamura, Kosuke; Teshima, Reiko; Nakashima, Akie; Ogawa, Asako; Yamagishi, Toru; Futo, Satoshi; Oguchi, Taichi; Mano, Junichi; Kitta, Kazumi

    2011-01-01

    In many countries, the labeling of grains, feed, and foodstuff is mandatory if the genetically modified (GM) organism content exceeds a certain level of approved GM varieties. We previously developed an individual kernel detection system consisting of grinding individual kernels, DNA extraction from the individually ground kernels, GM detection using multiplex real-time PCR, and GM event detection using multiplex qualitative PCR to analyze the precise commingling level and varieties of GM maize in real sample grains. We performed the interlaboratory study of the DNA extraction with multiple ground samples, multiplex real-time PCR detection, and multiplex qualitative PCR detection to evaluate its applicability, practicality, and ruggedness for the individual kernel detection system of GM maize. DNA extraction with multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR were evaluated by five laboratories in Japan, and all results from these laboratories were consistent with the expected results in terms of the commingling level and event analysis. Thus, the DNA extraction with multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR for the individual kernel detection system is applicable and practicable in a laboratory to regulate the commingling level of GM maize grain for GM samples, including stacked GM maize.

  4. Interlaboratory study of DNA extraction from multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR for individual kernel detection system of genetically modified maize.

    PubMed

    Akiyama, Hiroshi; Sakata, Kozue; Makiyma, Daiki; Nakamura, Kosuke; Teshima, Reiko; Nakashima, Akie; Ogawa, Asako; Yamagishi, Toru; Futo, Satoshi; Oguchi, Taichi; Mano, Junichi; Kitta, Kazumi

    2011-01-01

    In many countries, the labeling of grains, feed, and foodstuff is mandatory if the genetically modified (GM) organism content exceeds a certain level of approved GM varieties. We previously developed an individual kernel detection system consisting of grinding individual kernels, DNA extraction from the individually ground kernels, GM detection using multiplex real-time PCR, and GM event detection using multiplex qualitative PCR to analyze the precise commingling level and varieties of GM maize in real sample grains. We performed the interlaboratory study of the DNA extraction with multiple ground samples, multiplex real-time PCR detection, and multiplex qualitative PCR detection to evaluate its applicability, practicality, and ruggedness for the individual kernel detection system of GM maize. DNA extraction with multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR were evaluated by five laboratories in Japan, and all results from these laboratories were consistent with the expected results in terms of the commingling level and event analysis. Thus, the DNA extraction with multiple ground samples, multiplex real-time PCR, and multiplex qualitative PCR for the individual kernel detection system is applicable and practicable in a laboratory to regulate the commingling level of GM maize grain for GM samples, including stacked GM maize. PMID:22165018

  5. Performance of the prototype readout system for the CMS endcap hadron calorimeter upgrade

    NASA Astrophysics Data System (ADS)

    Pastika, N. J.

    2016-03-01

    The CMS experiment at the CERN Large Hadron Collider (LHC) will upgrade the photon detection and readout systems of its barrel and endcap hadron calorimeters (HCAL) through the second long shutdown of the LHC in 2018. The upgrade includes new silicon photomultipliers (SiPMs), SiPM control electronics, signal digitization via the Fermilab QIE11 ASIC, data formatting and serialization via a Microsemi FPGA, and data transmission via CERN Versatile Link technology. The first prototype system for the endcap HCAL has been assembled and characterized on the bench and in a test beam. The design of this new system and prototype performance are described.

  6. Upgrade of the TOTEM DAQ using the Scalable Readout System (SRS)

    NASA Astrophysics Data System (ADS)

    Quinto, M.; Cafagna, F.; Fiergolski, A.; Radicioni, E.

    2013-11-01

    The main goals of the TOTEM Experiment at the LHC are the measurements of the elastic and total p-p cross sections and the studies of the diffractive dissociation processes. At LHC, collisions are produced at a rate of 40 MHz, imposing strong requirements for the Data Acquisition Systems (DAQ) in terms of trigger rate and data throughput. The TOTEM DAQ adopts a modular approach that, in standalone mode, is based on VME bus system. The VME based Front End Driver (FED) modules, host mezzanines that receive data through optical fibres directly from the detectors. After data checks and formatting are applied in the mezzanine, data is retransmitted to the VME interface and to another mezzanine card plugged in the FED module. The VME bus maximum bandwidth limits the maximum first level trigger (L1A) to 1 kHz rate. In order to get rid of the VME bottleneck and improve scalability and the overall capabilities of the DAQ, a new system was designed and constructed based on the Scalable Readout System (SRS), developed in the framework of the RD51 Collaboration. The project aims to increase the efficiency of the actual readout system providing higher bandwidth, and increasing data filtering, implementing a second-level trigger event selection based on hardware pattern recognition algorithms. This goal is to be achieved preserving the maximum back compatibility with the LHC Timing, Trigger and Control (TTC) system as well as with the CMS DAQ. The obtained results and the perspectives of the project are reported. In particular, we describe the system architecture and the new Opto-FEC adapter card developed to connect the SRS with the FED mezzanine modules. A first test bench was built and validated during the last TOTEM data taking period (February 2013). Readout of a set of 3 TOTEM Roman Pot silicon detectors was carried out to verify performance in the real LHC environment. In addition, the test allowed a check of data consistency and quality.

  7. Selection of chromosomal DNA libraries using a multiplex CRISPR system

    PubMed Central

    Ryan, Owen W; Skerker, Jeffrey M; Maurer, Matthew J; Li, Xin; Tsai, Jordan C; Poddar, Snigdha; Lee, Michael E; DeLoache, Will; Dueber, John E; Arkin, Adam P; Cate, Jamie HD

    2014-01-01

    The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the use of complex marker-dependent chromosomal integration strategies. We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae genome by optimizing an efficient single-step and marker-free genome editing system using CRISPR-Cas9. With this Multiplex CRISPR (CRISPRm) system, we selected an improved cellobiose utilization pathway in diploid yeast in a single round of mutagenesis and selection, which increased cellobiose fermentation rates by over 10-fold. Mutations recovered in the best cellodextrin transporters reveal synergy between substrate binding and transporter dynamics, and demonstrate the power of CRISPRm to accelerate selection experiments and discoveries of the molecular determinants that enhance biomolecule function. DOI: http://dx.doi.org/10.7554/eLife.03703.001 PMID:25139909

  8. A readout system-on-chip for a cubic kilometer submarine neutrino telescope

    NASA Astrophysics Data System (ADS)

    Le Provost, H.; Moudden, Y.; Anvar, S.; Château, F.; Gautard, V.; Louis, F.; Ménager, K.; Vallage, B.; Zonca, E.

    2011-12-01

    This contribution reports on the read-out system of the future KM3NeT undersea network of several thousands of synchronized optical detecting nodes. Each node embeds a specifically designed fully integrated communicating system based on Xilinx FPGA SoC technology. It runs the VxWorks real-time OS and DAQ software designed within the Ice middleware framework resulting in a highly flexible, controllable and scalable distributed application. Clock distribution and delay calibration over customized fixed latency gigabit Ethernet links enable synchronous time stamping of events with nanosecond precision.

  9. A simple wavelength division multiplexing system for active learning teaching

    NASA Astrophysics Data System (ADS)

    Zghal, Mourad; Ghalila, Hassen; Ben Lakhdar, Zohra

    2009-06-01

    The active learning project consists in a series of workshops for educators, researchers and students and promotes an innovative method of teaching physics using simple, inexpensive materials that can be fabricated locally. The objective of the project is to train trainers and inspire students to learn physics. The workshops are based on the use of laboratory work and hands-on activities in the classroom. The interpretation of these experiments is challenging for some students, and the experiments can lead to a significant amount of discussion. The workshops are organized within the framework of the project ``Active Learning in Optics and Photonics" (ALOP) mainly funded by UNESCO, with the support of ICTP (Abdus Salam International Centre for Theoretical Physics) and SPIE. ALOP workshops offer high school, college or university physics teachers the opportunity to improve their conceptual understanding of optics. These workshops usually run for five days and cover several of the topics usually found in any introductory university physics program. Optics and photonics are used as subject matter because it is relevant as well as adaptable to research and educational conditions in many developing countries [1]. In this paper, we will mainly focus on a specific topic of the ALOP workshops, namely optical communications and Wavelength Division Multiplexing technology (WDM). This activity was originally developed by Mazzolini et al [2]. WDM is a technology used in fibre-optic communications for transmitting two or more separate signals over a single fibre optic cable by using a separate wavelength for each signal. Multiple signals are carried together as separate wavelengths of light in a multiplexed signal. Simple and inexpensive WDM system was implemented in our laboratory using light emitting diodes or diode lasers, plastic optical fibres, a set of optical filters and lenses, prism or grating, and photodiodes. Transmission of audio signals using home-made, simple

  10. Image processing system design for microcantilever-based optical readout infrared arrays

    NASA Astrophysics Data System (ADS)

    Tong, Qiang; Dong, Liquan; Zhao, Yuejin; Gong, Cheng; Liu, Xiaohua; Yu, Xiaomei; Yang, Lei; Liu, Weiyu

    2012-12-01

    Compared with the traditional infrared imaging technology, the new type of optical-readout uncooled infrared imaging technology based on MEMS has many advantages, such as low cost, small size, producing simple. In addition, the theory proves that the technology's high thermal detection sensitivity. So it has a very broad application prospects in the field of high performance infrared detection. The paper mainly focuses on an image capturing and processing system in the new type of optical-readout uncooled infrared imaging technology based on MEMS. The image capturing and processing system consists of software and hardware. We build our image processing core hardware platform based on TI's high performance DSP chip which is the TMS320DM642, and then design our image capturing board based on the MT9P031. MT9P031 is Micron's company high frame rate, low power consumption CMOS chip. Last we use Intel's company network transceiver devices-LXT971A to design the network output board. The software system is built on the real-time operating system DSP/BIOS. We design our video capture driver program based on TI's class-mini driver and network output program based on the NDK kit for image capturing and processing and transmitting. The experiment shows that the system has the advantages of high capturing resolution and fast processing speed. The speed of the network transmission is up to 100Mbps.

  11. 21 CFR 862.2570 - Instrumentation for clinical multiplex test systems.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... be compatible with more than one specific assay. The device includes a signal reader unit, and may.... Instrumentation for clinical multiplex test systems is a device intended to measure and sort multiple...

  12. 21 CFR 862.2570 - Instrumentation for clinical multiplex test systems.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... be compatible with more than one specific assay. The device includes a signal reader unit, and may.... Instrumentation for clinical multiplex test systems is a device intended to measure and sort multiple...

  13. 21 CFR 862.2570 - Instrumentation for clinical multiplex test systems.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... be compatible with more than one specific assay. The device includes a signal reader unit, and may.... Instrumentation for clinical multiplex test systems is a device intended to measure and sort multiple...

  14. Trigger-less readout system with pulse pile-up recovery for the PANDA electromagnetic calorimeter

    NASA Astrophysics Data System (ADS)

    Kavatsyuk, M.; Tambave, G.; Hevinga, M.; Lemmens, P. J. J.; Schakel, P.; Schreuder, F.; Speelman, R.; Löhner, H.; Panda Collaboration

    2013-08-01

    A simple, efficient, and robust on-line data-processing scheme was developed for the digital front-end electronics of the electromagnetic calorimeter of the PANDA spectrometer at FAIR, Darmstadt. The implementation of the processing algorithm in FPGA enables the construction of an almost dead-time free data acquisition system. The prototype of a complete trigger-less readout chain has been developed and evaluated. The precision of time synchronisation commands has been verified. A pile-up recovery algorithm was developed and evaluated over a large dynamic range of signal amplitudes.

  15. Multiplex immune serum biomarker profiling in sarcoidosis and systemic sclerosis.

    PubMed

    Beirne, P; Pantelidis, P; Charles, P; Wells, A U; Abraham, D J; Denton, C P; Welsh, K I; Shah, P L; du Bois, R M; Kelleher, P

    2009-12-01

    Multiplex protein technology has the potential to identify biomarkers for the differentiation, classification and improved understanding of the pathogenesis of interstitial lung disease. The aim of this study was to determine whether a 30-inflammatory biomarker panel could discriminate between healthy controls, sarcoidosis and systemic sclerosis (SSc) patients independently of other clinical indicators. We also evaluated whether a panel of biomarkers could differentiate between the presence or absence of lung fibrosis in SSc patients. We measured 30 circulating biomarkers in 20 SSc patients, 21 sarcoidosis patients and 20 healthy controls using Luminex bead technology and used Fisher's discriminant function analysis to establish the groups of classification mediators. There were significant differences in median concentration measurements between study groups for 20 of the mediators but with considerable range overlap between the groups, limiting group differentiation by single analyte measurements. However, a 17-analyte biomarker model correctly classified 90% of study individuals to their respective group and another 14-biomarker panel correctly identified the presence of lung fibrosis in SSc patients. These findings, if they are corroborated by independent studies in other centres, have potential for clinical application and may generate novel insights into the modulation of immune profiles during disease evolution. PMID:19541722

  16. Optical add/drop filter for wavelength division multiplexed systems

    DOEpatents

    Deri, Robert J.; Strand, Oliver T.; Garrett, Henry E.

    2002-01-01

    An optical add/drop filter for wavelength division multiplexed systems and construction methods are disclosed. The add/drop filter includes a first ferrule having a first pre-formed opening for receiving a first optical fiber; an interference filter oriented to pass a first set of wavelengths along the first optical fiber and reflect a second set of wavelengths; and, a second ferrule having a second pre-formed opening for receiving the second optical fiber, and the reflected second set of wavelengths. A method for constructing the optical add/drop filter consists of the steps of forming a first set of openings in a first ferrule; inserting a first set of optical fibers into the first set of openings; forming a first set of guide pin openings in the first ferrule; dividing the first ferrule into a first ferrule portion and a second ferrule portion; forming an interference filter on the first ferrule portion; inserting guide pins through the first set of guide pin openings in the first ferrule portion and second ferrule portion to passively align the first set of optical fibers; removing material such that light reflected from the interference filter from the first set of optical fibers is accessible; forming a second set of openings in a second ferrule; inserting a second set of optical fibers into the second set of openings; and positioning the second ferrule with respect to the first ferrule such that the second set of optical fibers receive the light reflected from the interference filter.

  17. Analog filtering methods improve leading edge timing performance of multiplexed SiPMs

    NASA Astrophysics Data System (ADS)

    Bieniosek, M. F.; Cates, J. W.; Grant, A. M.; Levin, C. S.

    2016-08-01

    Multiplexing many SiPMs to a single readout channel is an attractive option to reduce the readout complexity of high performance time of flight (TOF) PET systems. However, the additional dark counts and shaping from each SiPM cause significant baseline fluctuations in the output waveform, degrading timing measurements using a leading edge threshold. This work proposes the use of a simple analog filtering network to reduce the baseline fluctuations in highly multiplexed SiPM readouts. With 16 SiPMs multiplexed, the FWHM coincident timing resolution for single 3~\\text{mm}× 3~\\text{mm}× 20 mm LYSO crystals was improved from 401  ±  4 ps without filtering to 248  ±  5 ps with filtering. With 4 SiPMs multiplexed, using an array of 3~\\text{mm}× 3~\\text{mm}× 20 mm LFS crystals the mean time resolution was improved from 436  ±  6 ps to 249  ±  2 ps. Position information was acquired with a novel binary positioning network. All experiments were performed at room temperature with no active temperature regulation. These results show a promising technique for the construction of high performance multiplexed TOF PET readout systems using analog leading edge timing pickoff.

  18. Analog filtering methods improve leading edge timing performance of multiplexed SiPMs.

    PubMed

    Bieniosek, M F; Cates, J W; Grant, A M; Levin, C S

    2016-08-21

    Multiplexing many SiPMs to a single readout channel is an attractive option to reduce the readout complexity of high performance time of flight (TOF) PET systems. However, the additional dark counts and shaping from each SiPM cause significant baseline fluctuations in the output waveform, degrading timing measurements using a leading edge threshold. This work proposes the use of a simple analog filtering network to reduce the baseline fluctuations in highly multiplexed SiPM readouts. With 16 SiPMs multiplexed, the FWHM coincident timing resolution for single [Formula: see text] mm LYSO crystals was improved from 401  ±  4 ps without filtering to 248  ±  5 ps with filtering. With 4 SiPMs multiplexed, using an array of [Formula: see text] mm LFS crystals the mean time resolution was improved from 436  ±  6 ps to 249  ±  2 ps. Position information was acquired with a novel binary positioning network. All experiments were performed at room temperature with no active temperature regulation. These results show a promising technique for the construction of high performance multiplexed TOF PET readout systems using analog leading edge timing pickoff.

  19. Final Report Nucleic Acid System - Hybrid PCR and Multiplex Assay Project Phase 2

    SciTech Connect

    Koopman, R P; Langlois, R G; Nasarabadi, S; Benett, W J; Colston, B W; Johnson, D C; Brown, S B; Stratton, P L; Milanovich, F P

    2002-04-17

    This report covers phase 2 (year 2) of the Nucleic Acid System--Hybrid PCR and Multiplex Assay project. The objective of the project is to reduce to practice the detection and identification of biological warfare pathogens by the nucleic acid recognition technique of PCR (polymerase chain reaction) in a multiplex mode using flow cytometry. The Hybrid instrument consists of a flow-through PCR module capable of handling a multiplexed PCR assay, a hybridizing module capable of hybridizing multiplexed PCR amplicons and beads, and a flow cytometer module for bead-based identification, all controlled by a single computer. Multiplex immunoassay using bead-based Luminex flow cytometry is available, allowing rapid screening for many agents. PCR is highly specific and complements and verifies immunoassay. It can also be multiplexed and detection provided using the bead-based Luminex flow cytometer. This approach allows full access to the speed and 100-fold multiplex capability of flow cytometry for rapid screening as well as the accuracy and specificity of PCR. This project has two principal activities: (1) Design, build and test a prototype hybrid PCR/flow cytometer with the basic capabilities for rapid, broad spectrum detection and identification, and (2) Develop and evaluate multiplex flow analysis assay protocols and reagents for the simultaneous detection of PCR products. This project requires not only building operationally functional instrumentation but also developing the chemical assays for detection of priority pathogens. This involves development and evaluation of multiplex flow analysis assay protocols and reagents for the simultaneous detection of PCR products.

  20. A microfluidic device for the automated electrical readout of low-density glass-slide microarrays.

    PubMed

    Díaz-González, María; Salvador, J Pablo; Bonilla, Diana; Marco, M Pilar; Fernández-Sánchez, César; Baldi, Antoni

    2015-12-15

    Microarrays are a powerful platform for rapid and multiplexed analysis in a wide range of research fields. Electrical readout systems have emerged as an alternative to conventional optical methods for microarray analysis thanks to its potential advantages like low-cost, low-power and easy miniaturization of the required instrumentation. In this work an automated electrical readout system for low-cost glass-slide microarrays is described. The system enables the simultaneous conductimetric detection of up to 36 biorecognition events by incorporating an array of interdigitated electrode transducers. A polydimethylsiloxane microfluidic structure has been designed that creates microwells over the transducers and incorporates the microfluidic channels required for filling and draining them with readout and cleaning solutions, thus making the readout process fully automated. Since the capture biomolecules are not immobilized on the transducer surface this readout system is reusable, in contrast to previously reported electrochemical microarrays. A low-density microarray based on a competitive enzymatic immunoassay for atrazine detection was used to test the performance of the readout system. The electrical assay shows a detection limit of 0.22±0.03 μg L(-1) similar to that obtained with fluorescent detection and allows the direct determination of the pesticide in polluted water samples. These results proved that an electrical readout system such as the one presented in this work is a reliable and cost-effective alternative to fluorescence scanners for the analysis of low-density microarrays.

  1. Subcarrier multiplexing system with built-in dispersion reduction

    SciTech Connect

    Sargis, P.D.; Haigh, R.E.; McCammon, K.G.

    1995-09-08

    Dispersion is effectively reduced in a 1550-nm subcarrier-multiplexed fiber link by using optical pre-filtering at the receiver. Recent experimental results demonstrate transmission of two 2.5 Gbit/s data channels over 220 km of ordinary single-mode fiber.

  2. SiPM based readout system for PbWO4 crystals

    NASA Astrophysics Data System (ADS)

    Berra, A.; Bolognini, D.; Bonfanti, S.; Bonvicini, V.; Lietti, D.; Penzo, A.; Prest, M.; Stoppani, L.; Vallazza, E.

    2013-08-01

    Silicon PhotoMultipliers (SiPMs) consist of a matrix of small passively quenched silicon avalanche photodiodes operated in limited Geiger-mode (GM-APDs) and read out in parallel from a common output node. Each pixel (with a typical size in the 20-100 μm range) gives the same current response when hit by a photon; the SiPM output signal is the sum of the signals of all the pixels, which depends on the light intensity. The main advantages of SiPMs with respect to photomultiplier tubes (PMTs) are essentially the small dimensions, the insensitivity to magnetic fields and a low bias voltage. This contribution presents the performance of a SiPM based readout system for crystal calorimeters developed in the framework of the FACTOR/TWICE collaboration. The SiPM used for the test is a new device produced by FBK-irst which consists in a matrix of four sensors embedded in the same silicon substrate, called QUAD. The SiPM has been coupled to a lead tungstate crystal, an early-prototype version of the crystals developed for the electromagnetic calorimeter of the CMS experiment. New tests are foreseen using a complete module consisting of nine crystals, each one readout by two QUADs.

  3. Custom solution for a data readout architecture: A system level simulation

    SciTech Connect

    Aloisio, A.; Cevenini, F.; Volpe, D. della; Merola, L.; Parascandolo, P.

    1994-12-31

    Behavioral simulations of data readout architecture based on VME and custom high speed buses show that it is suitable as data acquisition and event building system for high energy physics experiments. This paper describes a reliable but simple auxiliary bus designed to afford asynchronous transactions up to 10 Mtransfers/s, sparse data scan operations and crate-level event building. An intercate connection is also presented to accomplish system level event building and data concentration by means of synchronous transactions of rates up to 10 Mtransfers/s. This architecture has been simulated using Verilog HDL. Preliminary performance estimates are presented and briefly discussed in view of system application in KLOE experiment at the DA{Phi}NE {Phi}-factory in Frascati (Italy).

  4. The prototype readout electronics system for the External Target Experiment in CSR of HIRFL

    NASA Astrophysics Data System (ADS)

    Zhao, L.; Kang, L.; Li, M.; Liu, S.; Zhou, J.; An, Q.

    2014-07-01

    A prototype readout electronics system was designed for the External Target Experiment in the Cooling Storage Ring (CSR) of the Heavy Ion Research Facility in Lanzhou (HIRFL). The kernel parts include the 128-channel 100 ps high-resolution time digitization module, the 16-channel 25 ps high-resolution time and charge measurement module, and the trigger electronics, as well as the clock generation circuits, which are all integrated within the PXI-6U crate. The laboratory test results indicate that a good resolution is achieved, better than the requirement. We also have conducted initial commissioning tests with the detectors to confirm the functions of the system. Through the research of this prototype electronics, preparation for the future extended system is made.

  5. On the Integration of a Readout System Dedicated for Neutron Discrimination in Harsh Environment

    NASA Astrophysics Data System (ADS)

    Ben Krit, S.; Rahajandraibe, W.; Coulié-Castellani, K.; Micolau, G.; Lyoussi, A.

    2016-02-01

    New insights related to the integration of a readout system dedicated for the detection and discrimination of neutrons are presented here. This study takes place in the framework of the I_SMART European project. This system will have to work later in a harsh environment in terms of temperature and radiations, what makes not only the development of specifications for operation and reliability of the components necessary but also the investigation of margins for the interplay of the system. Implementation of the analog conditioning chain at transistor level (AMS (Analog/Mixed Signal) 0.35μm CMOS technology) is investigated here where electrical performances have been validated at SPICE-level simulations using "Spectre" simulator (SPICE-based) under Cadence DFII.

  6. Detection Systems and Algorithms for Multiplexed Quantum Dots

    NASA Astrophysics Data System (ADS)

    Goss, Kelly Christine

    Quantum Dots (QDs) are semiconductor nanocrystals that absorb light and re-emit at a wavelength dependent on its size and shape. A group of quantum dots can be designed to have a unique spectral emission by varying the size of the quantum dots (wavelength) and number of quantum dots (optical power) [1]. This technology is refered to as Multiplexed Quantum Dots (MxQD) and when it was first proposed, MxQD tags were created with 6 optical power levels and one QD colour or 3 QD colours and 2 optical power levels. It was hypothesized that a realistic limit to the number of tags would be a system of 6 optical power levels and 6 QD colours resulting in 46655 unique tags. In recent work, the fabrication and detection of 9 unique tags [2] was demonstrated which is still far from the predicted capability of the technology. The limitations affecting the large number of unique tags are both the fabrication methods and the data detection algorithms used to read the spectral emissions. This thesis makes contributions toward improving the data detection algorithms for MxQD tags. To accomplish this, a communications system model is developed that includes the inteference between QD colours, Inter-Symbol Interference (ISI), and additive noise. The model is developed for the two optical detectors, namely a Charge-Coupled Device (CCD) spectrometer and photodiode detectors. The model also includes an analytical expression for the Signal-to-Noise Ratio (SNR) of the detectors. For the CCD spectrometer, this model is verified with an experimental prototype. With the models in place, communications systems tools are applied that overcome both ISI and noise. This is an improvement over previous work in the field that only considered algorithms to overcome the ISI or noise separately. Specifically, this thesis outlines the proposal of a matched filter to improve SNR, a Minimum Mean Square Error (MMSE) equalizer that mitigates ISI in the presence of noise and a Maximum Likelihood Sequence

  7. Performance of the Prototype Readout System for the CMS Endcap Hadron Calorimeter Upgrade

    NASA Astrophysics Data System (ADS)

    Chaverin, Nate; Dittmann, Jay; Hatakeyama, Kenichi; Pastika, Nathaniel; CMS Collaboration

    2016-03-01

    The Compact Muon Solenoid (CMS) experiment at the CERN Large Hadron Collider (LHC) will upgrade the photodetectors and readout systems of the endcap hadron calorimeter during the technical stop scheduled for late 2016 and early 2017. A major milestone for this project was a highly successful testbeam run at CERN in August 2015. The testbeam run served as a full integration test of the electronics, allowing a study of the response of the preproduction electronics to the true detector light profile, as well as a test of the light yield of various new plastic scintillator materials. We present implications for the performance of the hadron calorimeter front-end electronics based on testbeam data, and we report on the production status of various components of the system in preparation for the upgrade.

  8. In vivo simultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system

    NASA Astrophysics Data System (ADS)

    Lv, Yanlu; Zhang, Jiulou; Zhang, Dong; Cai, Wenjuan; Chen, Nanguang; Luo, Jianwen

    2016-06-01

    A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in the scene. The implementation and performance of the simultaneous multispectral imaging system are presented. Further, the system's capability in simultaneously obtaining multispectral fluorescence measurements is demonstrated with in vivo experiments on a mouse. The demonstrated imaging system has the potential to obtain multispectral images fluorescence simultaneously.

  9. Fast imaging readout and electronics—a novel high-speed imaging system for micro-channel plates

    NASA Astrophysics Data System (ADS)

    Lapington, J. S.; Rees, K.

    2002-01-01

    The band-width of charge division readout anodes used with micro-channel plates (MCP) is usually limited by the speed of the acquisition electronics. We present a novel charge division anode that does not require analogue to digital conversion. The Fast Imaging Readout and Electronics is a new concept in high-speed imaging using an MCP detector. The imaging system described comprises an MCP intensifier coupled to a charge division image readout using high-speed, multichannel electronics. It has a projected spatial resolution of up to 128×128 pixels, though the image format is inherently flexible, and the potential for rates up to 100 million events per second with nanosecond timing resolution. The readout pattern has a planar electrode structure and the collected charge from each event is shared amongst all electrodes, grouped in pairs. The unique design of the readout obviates the need for charge measurement, usually the dominant process determining the event-processing deadtime. Instead, high-speed signal comparators, each of which act on the signals from an electrode pair, are used to define a binary code from which the position co-ordinate is directly mapped. We describe a proof of the concept of prototype anode and associated electronics using a novel application of very high-speed digital circuitry. We present preliminary results showing signal waveforms measured using a one-dimensional 16-pixel anode pattern.

  10. Multiplexed optical operation of nanoelectromechanical systems (NEMS) arrays for sensing and signal-processing applications

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin

    2014-06-01

    NEMS are rapidly being developed for a variety of sensing applications as well as for exploring interesting regimes in fundamental physics. In most of these endeavors, operation of a NEMS device involves actuating the device harmonically around its fundamental resonance and detecting subsequent motion while the device interacts with its environment. Even though a single NEMS resonator is exceptionally sensitive, a typical application, such as sensing or signal processing, requires the detection of signals from many resonators distributed over the surface of a chip. Therefore, one of the key technological challenges in the field of NEMS is development of multiplexed measurement techniques to detect the motion of a large number of NEMS resonators simultaneously. In this work, we address the important and difficult problem of interfacing with a large number of NEMS devices and facilitating the use of such arrays in, for example, sensing and signal processing applications. We report a versatile, all-optical technique to excite and read-out a distributed NEMS array. The NEMS array is driven by a distributed, intensity-modulated, optical pump through the photothermal effect. The ensuing vibrational response of the array is multiplexed onto a single, probe beam as a high-frequency phase modulation. The phase modulation is optically down converted to a low-frequency, intensity modulation using an adaptive full -field interferometer, and subsequently is detected using a charge-coupled device (CCD) array. Rapid and single-step mechanical characterization of approximately 60 nominally identical, high-frequency resonators is demonstrated. The technique may enable sensitivity improvements over single NEMS resonators by averaging signals coming from a multitude of devices in the array. In addition, the diffraction-limited spatial resolution may allow for position-dependent read-out of NEMS sensor chips for sensing multiple analytes or spatially inhomogeneous forces.

  11. New conversion factors between human and automatic readouts of the CDMAM phantom for CR systems

    NASA Astrophysics Data System (ADS)

    Hummel, Johann; Homolka, Peter; Osanna-Elliot, Angelika; Kaar, Marcus; Semtrus, Friedrich; Figl, Michael

    2016-03-01

    Mammography screenings demand for profound image quality (IQ) assessment to guarantee their screening success. The European protocol for the quality control of the physical and technical aspects of mammography screening (EPQCM) suggests a contrast detail phantom such as the CDMAM phantom to evaluate IQ. For automatic evaluation a software is provided by the EUREF. As human and automatic readouts differ systematically conversion factors were published by the official reference organisation (EUREF). As we experienced a significant difference for these factors for Computed Radiography (CR) systems we developed an objectifying analysis software which presents the cells including the gold disks randomly in thickness and rotation. This allows to overcome the problem of an inevitable learning effect where observers know the position of the disks in advance. Applying this software, 45 computed radiography (CR) systems were evaluated and the conversion factors between human and automatic readout determined. The resulting conversion factors were compared with the ones resulting from the two methods published by EUREF. We found our conversion factors to be substantially lower than those suggested by EUREF, in particular 1.21 compared to 1.42 (EUREF EU method) and 1.62 (EUREF UK method) for 0.1 mm, and 1.40 compared to 1.73 (EUREF EU) and 1.83 (EUREF UK) for 0.25 mm disc diameter, respectively. This can result in a dose increase of up to 90% using either of these factors to adjust patient dose in order to fulfill image quality requirements. This suggests the need of an agreement on their proper application and limits the validity of the assessment methods. Therefore, we want to stress the need for clear criteria for CR systems based on appropriate studies.

  12. A low-cost, multiplexed electrophysiology system for chronic μECoG recordings in rodents.

    PubMed

    Wang, JuiChih; Trumpis, Michael; Insanally, Michele; Froemke, Robert; Viventi, Jonathan

    2014-01-01

    Micro-Electrocorticography (μECoG) offers a minimally invasive, high resolution interface with large areas of cortex. However, large arrays of electrodes with many contacts that are individually wired to external recording systems are cumbersome and make chronic recording in freely behaving small animals challenging. Multiplexed headstages overcome this limitation by combining the signals from many electrodes to a smaller number of connections directly on the animal's head. Commercially available multiplexed headstages provide high performance integrated amplification, multiplexing and analog to digital conversion. However, the cost of these systems can be prohibitive for small labs or for experiments that require a large number of animals to be continuously recorded at the same time. Here we have developed a multiplexed 60-channel headstage amplifier optimized to chronically record electrophysiological signals from high-density μECoG electrode arrays. A single, ultraflexible (2 mm thickness) microHDMI cable provided the data interface. Using low cost components, we have reduced the cost of the multiplexed headstage to ~$125. Paired with a custom interface printed circuit board (PCB) and a general purpose data acquisition system (M-series DAQ, National Instruments), an inexpensive and customizable electrophysiology system is assembled. Open source LabVIEW software that we have previously released controlled the system. It can also be used with other open source neural data acquisition packages. Combined, we have presented a scalable, low-cost platform for high-channel count electrophysiology.

  13. Finite element simulations of low-mass readout cables for the CBM Silicon Tracking System using RAPHAEL

    NASA Astrophysics Data System (ADS)

    Singla, M.; Chatterji, S.; Müller, W. F. J.; Kleipa, V.; Heuser, J. M.

    2014-01-01

    The first three-dimensional simulation study of thin multi-line readout cables using finite element simulation tool RAPHAEL is being reported. The application is the Silicon Tracking System (STS) of the fixed-target heavy-ion experiment Compressed Baryonic Matter (CBM), under design at the forthcoming accelerator center FAIR in Germany. RAPHAEL has been used to design low-mass analog readout cables with minimum possible Equivalent Noise Charge (ENC). Various trace geometries and trace materials have been explored in detail for this optimization study. These cables will bridge the distance between the microstrip detectors and the signal processing electronics placed at the periphery of the silicon tracking stations. SPICE modeling has been implemented in Sentaurus Device to study the transmission loss (dB loss) in cables and simulation has been validated with measurements. An optimized design having minimum possible ENC, material budget and transmission loss for the readout cables has been proposed.

  14. Status of the silicon strip high-rate FASTBUS readout system

    SciTech Connect

    Gonzalez, H.; Barsotti, E.; Bowden, M.; Christian, D.; Chramowicz, J.; Fachin, M.; Haldeman, M.; Hoff, J.; Holmes, S.; Rotolo, C.; Romero, A.; Slimmer, D.; Swoboda, C.; Trendler, R.; Urish, J.; Yarema, R.; Zimmerman, T.; Zimmermann, S.; Kowald, W.; MacManus, A.; Recagni, M.; Segal, J.; Spentzouris, P.

    1991-11-01

    Our new readout system was developed in collaboration with, and largely to the specification of, the E771 experimenters. E771 is a fixed target experiment designed to study the production of B hadrons by an 800 GeV/c proton beam. The experiment will operate at rates of up to 200 million beam protons per second and 10 million interactions per second. The experimental apparatus will consist of an open geometry magnetic spectrometer featuring good muon and electron identification (much of which was used in E705), and a compact 16000 channel Silicon Strip Detector. In order to satisfy the experimenter`s desire to instrument 16000 SSD elements in a package only 5 cm wide, 5 cm high, and 21 cm deep, and in order to meet the performance specifications, we have made extensive use of ``Application Specific Integrated Circuits`` (ASIC`s).

  15. Status of the silicon strip high-rate FASTBUS readout system

    SciTech Connect

    Gonzalez, H.; Barsotti, E.; Bowden, M.; Christian, D.; Chramowicz, J.; Fachin, M.; Haldeman, M.; Hoff, J.; Holmes, S.; Rotolo, C.; Romero, A.; Slimmer, D.; Swoboda, C.; Trendler, R.; Urish, J.; Yarema, R.; Zimmerman, T.; Zimmermann, S.; Kowald, W.; MacManus, A.; Recagni, M.; Segal, J.; Spentzouris, P.

    1991-11-01

    Our new readout system was developed in collaboration with, and largely to the specification of, the E771 experimenters. E771 is a fixed target experiment designed to study the production of B hadrons by an 800 GeV/c proton beam. The experiment will operate at rates of up to 200 million beam protons per second and 10 million interactions per second. The experimental apparatus will consist of an open geometry magnetic spectrometer featuring good muon and electron identification (much of which was used in E705), and a compact 16000 channel Silicon Strip Detector. In order to satisfy the experimenter's desire to instrument 16000 SSD elements in a package only 5 cm wide, 5 cm high, and 21 cm deep, and in order to meet the performance specifications, we have made extensive use of Application Specific Integrated Circuits'' (ASIC's).

  16. The front-end readout and DAQ system of LASGA in China space station

    NASA Astrophysics Data System (ADS)

    Guo, Jian hua; Gan, Weiqun; Hu, Yiming; Chang, Jin; Zhang, Zhe

    2016-07-01

    LASGA (Large Area Solar GAmma-ray spectrometer) , which will be installed in China space station, will cover the energy of X/Gamma ray between 10KeV to 2.5GeV. The energy resolution of LASGA is better than 2.8%@662kev and the spatial resolution is about 1.5 degree @1GeV. LASGA consists of anti-coincidence plastic scintillation detector, X-ray detector, silicon strip tracker and Gamma ray detector. The effective area of the detector is about 400mm*400mm. LASGA has two different observed target: solar X/Gamma ray and Gamma ray from galactic center, which depends on whether the space station is in the shadow area or not. In this paper, we will introduce the front-end readout and data acquisition system of LASGA.

  17. Focal plane infrared readout circuit

    NASA Technical Reports Server (NTRS)

    Pain, Bedabrata (Inventor)

    2002-01-01

    An infrared imager, such as a spectrometer, includes multiple infrared photodetectors and readout circuits for reading out signals from the photodetectors. Each readout circuit includes a buffered direct injection input circuit including a differential amplifier with active feedback provided through an injection transistor. The differential amplifier includes a pair of input transistors, a pair of cascode transistors and a current mirror load. Photocurrent from a photodetector can be injected onto an integration capacitor in the readout circuit with high injection efficiency at high speed. A high speed, low noise, wide dynamic range linear infrared multiplexer array for reading out infrared detectors with large capacitances can be achieved even when short exposure times are used. The effect of image lag can be reduced.

  18. Fiber optic multiplexed optical transmission systems for space vehicle launch facilities

    NASA Technical Reports Server (NTRS)

    Bell, C. H.

    1975-01-01

    Low loss Fiber Optic Cable is being evaluated as a potential future replacement for Kennedy Space Center's 13,000 mile Wideband cable system. In order to make economical use of the wide bandwidth characteristic of glass fibers, a Frequency Division Multiplexing (FDM) scheme has been devised to stack many analog and digital data channels on a single fiber. The Multiplexed Optical Transmission System (MOTS) will offer a unique flexibility of plug-in modularity to meet changing data and bandwidth requirements in addition to the standard 'goodies' of immunity to lightning and other EMI, RFI type interferences, and of smaller size and lighter weight.

  19. Multiplex detection of plant pathogens through the Luminex MagPlex bead system.

    PubMed

    van der Vlugt, René A A; van Raaij, Henry; de Weerdt, Marjanne; Bergervoet, Jan H W

    2015-01-01

    Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres ("beads"), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs. PMID:25981262

  20. Fluorescence-Raman Dual Modal Endoscopic System for Multiplexed Molecular Diagnostics

    PubMed Central

    Jeong, Sinyoung; Kim, Yong-il; Kang, Homan; Kim, Gunsung; Cha, Myeong Geun; Chang, Hyejin; Jung, Kyung Oh; Kim, Young-Hwa; Jun, Bong-Hyun; Hwang, Do Won; Lee, Yun-Sang; Youn, Hyewon; Lee, Yoon-Sik; Kang, Keon Wook; Lee, Dong Soo; Jeong, Dae Hong

    2015-01-01

    Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence, and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo, and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures. PMID:25820115

  1. Fluorescence-Raman Dual Modal Endoscopic System for Multiplexed Molecular Diagnostics

    NASA Astrophysics Data System (ADS)

    Jeong, Sinyoung; Kim, Yong-Il; Kang, Homan; Kim, Gunsung; Cha, Myeong Geun; Chang, Hyejin; Jung, Kyung Oh; Kim, Young-Hwa; Jun, Bong-Hyun; Hwang, Do Won; Lee, Yun-Sang; Youn, Hyewon; Lee, Yoon-Sik; Kang, Keon Wook; Lee, Dong Soo; Jeong, Dae Hong

    2015-03-01

    Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence, and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo, and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures.

  2. Analog signal multiplexing for PSAPD-based PET detectors: simulation and experimental validation

    PubMed Central

    Lau, Frances W Y; Vandenbroucke, Arne; Reynolds, Paul D; Olcott, Peter D; Horowitz, Mark A; Levin, Craig S

    2013-01-01

    A 1 mm3 resolution clinical positron emission tomography (PET) system employing 4608 position-sensitive avalanche photodiodes (PSAPDs) is under development. This paper describes a detector multiplexing technique that simplifies the readout electronics and reduces the density of the circuit board design. The multiplexing scheme was validated using a simulation framework that models the PSAPDs and front-end multiplexing circuits to predict the signal-to-noise ratio and flood histogram performance. Two independent experimental setups measured the energy resolution, time resolution, crystal identification ability and count rate both with and without multiplexing. With multiplexing, there was no significant degradation in energy resolution, time resolution and count rate. There was a relative 6.9 ± .0% and 9.4 ± 1.0% degradation in the figure of merit that characterizes the crystal identification ability observed in the measured and simulated ceramic-mounted PSAPD module flood histograms, respectively. PMID:21081831

  3. A front-end readout Detector Board for the OpenPET electronics system

    NASA Astrophysics Data System (ADS)

    Choong, W.-S.; Abu-Nimeh, F.; Moses, W. W.; Peng, Q.; Vu, C. Q.; Wu, J.-Y.

    2015-08-01

    We present a 16-channel front-end readout board for the OpenPET electronics system. A major task in developing a nuclear medical imaging system, such as a positron emission computed tomograph (PET) or a single-photon emission computed tomograph (SPECT), is the electronics system. While there are a wide variety of detector and camera design concepts, the relatively simple nature of the acquired data allows for a common set of electronics requirements that can be met by a flexible, scalable, and high-performance OpenPET electronics system. The analog signals from the different types of detectors used in medical imaging share similar characteristics, which allows for a common analog signal processing. The OpenPET electronics processes the analog signals with Detector Boards. Here we report on the development of a 16-channel Detector Board. Each signal is digitized by a continuously sampled analog-to-digital converter (ADC), which is processed by a field programmable gate array (FPGA) to extract pulse height information. A leading edge discriminator creates a timing edge that is ``time stamped'' by a time-to-digital converter (TDC) implemented inside the FPGA . This digital information from each channel is sent to an FPGA that services 16 analog channels, and then information from multiple channels is processed by this FPGA to perform logic for crystal lookup, DOI calculation, calibration, etc.

  4. A front-end readout Detector Board for the OpenPET electronics system

    DOE PAGESBeta

    Choong, W. -S.; Abu-Nimeh, F.; Moses, W. W.; Peng, Q.; Vu, C. Q.; Wu, J. -Y.

    2015-08-12

    Here, we present a 16-channel front-end readout board for the OpenPET electronics system. A major task in developing a nuclear medical imaging system, such as a positron emission computed tomograph (PET) or a single-photon emission computed tomograph (SPECT), is the electronics system. While there are a wide variety of detector and camera design concepts, the relatively simple nature of the acquired data allows for a common set of electronics requirements that can be met by a flexible, scalable, and high-performance OpenPET electronics system. The analog signals from the different types of detectors used in medical imaging share similar characteristics, whichmore » allows for a common analog signal processing. The OpenPET electronics processes the analog signals with Detector Boards. Here we report on the development of a 16-channel Detector Board. Each signal is digitized by a continuously sampled analog-to-digital converter (ADC), which is processed by a field programmable gate array (FPGA) to extract pulse height information. A leading edge discriminator creates a timing edge that is "time stamped" by a time-to-digital converter (TDC) implemented inside the FPGA. In conclusion, this digital information from each channel is sent to an FPGA that services 16 analog channels, and then information from multiple channels is processed by this FPGA to perform logic for crystal lookup, DOI calculation, calibration, etc.« less

  5. A front-end readout Detector Board for the OpenPET electronics system

    PubMed Central

    Choong, W.-S.; Abu-Nimeh, F.; Moses, W.W.; Peng, Q.; Vu, C.Q.; Wu, J.-Y.

    2016-01-01

    We present a 16-channel front-end readout board for the OpenPET electronics system. A major task in developing a nuclear medical imaging system, such as a positron emission computed tomograph (PET) or a single-photon emission computed tomograph (SPECT), is the electronics system. While there are a wide variety of detector and camera design concepts, the relatively simple nature of the acquired data allows for a common set of electronics requirements that can be met by a flexible, scalable, and high-performance OpenPET electronics system. The analog signals from the different types of detectors used in medical imaging share similar characteristics, which allows for a common analog signal processing. The OpenPET electronics processes the analog signals with Detector Boards. Here we report on the development of a 16-channel Detector Board. Each signal is digitized by a continuously sampled analog-to-digital converter (ADC), which is processed by a field programmable gate array (FPGA) to extract pulse height information. A leading edge discriminator creates a timing edge that is “time stamped” by a time-to-digital converter (TDC) implemented inside the FPGA. This digital information from each channel is sent to an FPGA that services 16 analog channels, and then information from multiple channels is processed by this FPGA to perform logic for crystal lookup, DOI calculation, calibration, etc. PMID:27134641

  6. A front-end readout Detector Board for the OpenPET electronics system

    SciTech Connect

    Choong, W. -S.; Abu-Nimeh, F.; Moses, W. W.; Peng, Q.; Vu, C. Q.; Wu, J. -Y.

    2015-08-12

    Here, we present a 16-channel front-end readout board for the OpenPET electronics system. A major task in developing a nuclear medical imaging system, such as a positron emission computed tomograph (PET) or a single-photon emission computed tomograph (SPECT), is the electronics system. While there are a wide variety of detector and camera design concepts, the relatively simple nature of the acquired data allows for a common set of electronics requirements that can be met by a flexible, scalable, and high-performance OpenPET electronics system. The analog signals from the different types of detectors used in medical imaging share similar characteristics, which allows for a common analog signal processing. The OpenPET electronics processes the analog signals with Detector Boards. Here we report on the development of a 16-channel Detector Board. Each signal is digitized by a continuously sampled analog-to-digital converter (ADC), which is processed by a field programmable gate array (FPGA) to extract pulse height information. A leading edge discriminator creates a timing edge that is "time stamped" by a time-to-digital converter (TDC) implemented inside the FPGA. In conclusion, this digital information from each channel is sent to an FPGA that services 16 analog channels, and then information from multiple channels is processed by this FPGA to perform logic for crystal lookup, DOI calculation, calibration, etc.

  7. A Framework for Analysis of Computational Imaging Systems: Role of Signal Prior, Sensor Noise and Multiplexing.

    PubMed

    Mitra, Kaushik; Cossairt, Oliver S; Veeraraghavan, Ashok

    2014-10-01

    Over the last decade, a number of computational imaging (CI) systems have been proposed for tasks such as motion deblurring, defocus deblurring and multispectral imaging. These techniques increase the amount of light reaching the sensor via multiplexing and then undo the deleterious effects of multiplexing by appropriate reconstruction algorithms. Given the widespread appeal and the considerable enthusiasm generated by these techniques, a detailed performance analysis of the benefits conferred by this approach is important. Unfortunately, a detailed analysis of CI has proven to be a challenging problem because performance depends equally on three components: (1) the optical multiplexing, (2) the noise characteristics of the sensor, and (3) the reconstruction algorithm which typically uses signal priors. A few recent papers [12], [30], [49] have performed analysis taking multiplexing and noise characteristics into account. However, analysis of CI systems under state-of-the-art reconstruction algorithms, most of which exploit signal prior models, has proven to be unwieldy. In this paper, we present a comprehensive analysis framework incorporating all three components. In order to perform this analysis, we model the signal priors using a Gaussian Mixture Model (GMM). A GMM prior confers two unique characteristics. First, GMM satisfies the universal approximation property which says that any prior density function can be approximated to any fidelity using a GMM with appropriate number of mixtures. Second, a GMM prior lends itself to analytical tractability allowing us to derive simple expressions for the `minimum mean square error' (MMSE) which we use as a metric to characterize the performance of CI systems. We use our framework to analyze several previously proposed CI techniques (focal sweep, flutter shutter, parabolic exposure, etc.), giving conclusive answer to the question: `How much performance gain is due to use of a signal prior and how much is due to

  8. Modified midi- and mini-multiplex PCR systems for mitochondrial DNA control region sequence analysis in degraded samples.

    PubMed

    Kim, Na Young; Lee, Hwan Young; Park, Sun Joo; Yang, Woo Ick; Shin, Kyoung-Jin

    2013-05-01

    Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60-year-old and 400-500-year-old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.

  9. A silicon photonic wavelength division multiplex system for high-speed data transmission in detector instrumentation

    NASA Astrophysics Data System (ADS)

    Skwierawski, P.; Schneider, M.; Karnick, D.; Eisenblätter, L.; Weber, M.

    2016-01-01

    We propose a new silicon photonics-based optical transmission system utilizing wavelength division multiplexing (WDM) . This technology has the possibility of reading out all raw data from a detector even without massive local data reduction. The transmitter in the detector volume consists of multiple integrated Mach-Zehnder modulators monolithically integrated with wavelength (de-)multiplexers. The first demonstrator currently under development aims for a data rate of 160 Gbit/s per fiber, scalable to 5 Tbit/s and beyond. We report on our recently developed Echelle grating WDM multiplexers with up to 45 channels on an area of 0.5 mm2 and electro-optic modulators providing a bandwidth of 18 GHz.

  10. 1 ns time to digital converters for the KM3NeT data readout system

    SciTech Connect

    Calvo, David [IFIC, Instituto de Física Corpuscular, CSIC- Universidad de Valencia, C Collaboration: KM3NeT Collaboration

    2014-11-18

    The KM3NeT collaboration aims at the construction of a multi-km3 high-energy neutrino telescope in the Mediterranean Sea consisting of thousands of glass spheres (nodes), each of them containing 31 photomultiplier (PMT) of small photocathode area. The readout and data acquisition system of KM3NeT has to collect, treat and send to shore, in an economic way, the enormous amount of data produced by the photomultipliers. For this purpose, 31 high-resolution time-interval measuring channels are implemented on the Field-Programmable Gate Arrays (FPGA) based on Time to Digital Converter (TDC). TDC are very common devices in particles physics experiments. Architectures with low resources occupancy are desirable allowing the implementation of other instrumentation, communication and synchronization systems on the same device. The required resolution to measure both, time of flight and timestamp must be 1 ns. A 4-Oversampling technique with two high frequency clocks is used to achieve this resolution. The proposed TDC firmware is developed using very few resources in Xilinx Kintex-7.

  11. Fiber faceplate modulation readout in Bi-material micro-cantilever mirror array imaging system

    NASA Astrophysics Data System (ADS)

    Hui, Mei; Xia, Zhengzheng; Liu, Ming; Dong, Liquan; Liu, Xiaohua; Zhao, Yuejin

    2016-05-01

    Fiber faceplate modulation was applied to read out the precise actuation of silicon-based, surface micro-fabricated cantilever mirrors array in optical imaging system. The faceplate was made by ordered bundles consisting of as many as ten thousands fibers. The transmission loss of an individual fiber in the bundles was 0.35dB/cm and the cross talk between neighboring fibers in the faceplate was about 15%. Micro-cantilever mirrors array (Focal-Plane Array (FPA)) which composed of two-level bi-material pixels, absorb incident infrared flux and result in a temperature increase. The temperature distribution of incident flux transformed to the deformation distribution in FPA which has a very big difference in coefficients of thermal expansion. FPA plays the roles of target sensing and has the characteristics of high detection sensitivity. Instead of general filter such as knife edge or pinhole, fiber faceplate modulate the beam reflected by the units of FPA. An optical readout signal brings a visible spectrum into pattern recognition system, yielding a visible image on monitor. Thermal images at room temperature have been obtained. The proposed method permits optical axis compact and image noise suppression.

  12. 21 CFR 862.2570 - Instrumentation for clinical multiplex test systems.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Instrumentation for clinical multiplex test systems. 862.2570 Section 862.2570 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES...

  13. Optical delay encoding for fast timing and detector signal multiplexing in PET

    PubMed Central

    Grant, Alexander M.; Levin, Craig S.

    2015-01-01

    Purpose: The large number of detector channels in modern positron emission tomography (PET) scanners poses a challenge in terms of readout electronics complexity. Multiplexing schemes are typically implemented to reduce the number of physical readout channels, but often result in performance degradation. Novel methods of multiplexing in PET must be developed to avoid this data degradation. The preservation of fast timing information is especially important for time-of-flight PET. Methods: A new multiplexing scheme based on encoding detector interaction events with a series of extremely fast overlapping optical pulses with precise delays is demonstrated in this work. Encoding events in this way potentially allows many detector channels to be simultaneously encoded onto a single optical fiber that is then read out by a single digitizer. A two channel silicon photomultiplier-based prototype utilizing this optical delay encoding technique along with dual threshold time-over-threshold is demonstrated. Results: The optical encoding and multiplexing prototype achieves a coincidence time resolution of 160 ps full width at half maximum (FWHM) and an energy resolution of 13.1% FWHM at 511 keV with 3 × 3 × 5 mm3 LYSO crystals. All interaction information for both detectors, including timing, energy, and channel identification, is encoded onto a single optical fiber with little degradation. Conclusions: Optical delay encoding and multiplexing technology could lead to time-of-flight PET scanners with fewer readout channels and simplified data acquisition systems. PMID:26233181

  14. Compensated digital readout family

    NASA Technical Reports Server (NTRS)

    Ludwig, David E.; Skow, Michael

    1991-01-01

    ISC has completed test on an IC which has 32 channels of amplifiers, low pass anti-aliasing filters, 13-bit analog-to-digital (A/D) converters with non-uniformity correction per channel and a digital multiplexer. The single slope class of A/D conversion is described, as are the unique variations required for incorporation of this technique for use with on-focal plane detector readout electronics. This paper describes the architecture used to implement the digital on-focal plane signal processing functions. Results from measured data on a test IC are presented for a circuit containing these functions operating at a sensor frame rate of 1000 hertz.

  15. A novel spatial-frequency-multiplexed fiber-optic Fizeau strain sensor system

    NASA Astrophysics Data System (ADS)

    Rao, Yun-Jiang; Jiang, Jian; Zhou, Chang X.

    2005-02-01

    A novel method for multiplexing fiber-optic Fizeau strain sensors with optical amplification is proposed and demonstrated. This method overcomes the two intrinsic disadvantages of fiber-optic Fabry-Perot (F-P) strain sensors, i.e. weak signal and difficult multiplexing. The amplified spontaneous emission (ASE) and optical amplification of an Erbium-doped fiber (EDF) pumped by a 980nm laser diode are used simultaneously to enhance the interferometric signal considerably. A Fizeau interferometer formed by two fiber ends with a quite different reflectivity is used to replace the F-P cavity in sensor head design. Such a Fizeau cavity can enlarge the cavity length by at least an order of magnitude and allows more than ten sensors to be multiplexed simultaneously by using spatial-frequency multiplexing. The operating principle of the sensor system is discussed and an experiment is carried out to verify the concept of the method proposed. It is anticipated that such a sensor system could find important applications for health monitoring of large structures.

  16. Microstrip electrode readout noise for load-dominated long shaping-time systems

    NASA Astrophysics Data System (ADS)

    Collier, Kelsey; Cunnington, Taylor; Crosby, Sean; Fadeyev, Vitaliy; Martinez-McKinney, Forest; Mistry, Khilesh; Schumm, Bruce A.; Spencer, Edwin; Taylor, Aaron; Wilder, Max

    2013-11-01

    In cases such as that of the proposed International Linear Collider (ILC), for which the beam-delivery and detector-occupancy characteristics permit a long shaping-time readout of the microstrip sensors, it is possible to envision long (∼1 meter) daisy-chained ‘ladders’ of fine-pitch sensors read out by a single front-end amplifier. In this study, a long shaping-time (∼2 μsec) front-end amplifier has been used to measure readout noise as a function of detector load. Comparing measured noise to that expected from lumped and distributed models of the load network, it is seen that network effects significantly mitigate the amount of readout noise contributed by the detector load. Further reduction in noise is demonstrated for the case that the sensor load is read out from its center rather than its end.

  17. Multiplexed Volume Bragg Gratings in Narrowand Broad-band Spectral Systems: Analysis and Application

    NASA Astrophysics Data System (ADS)

    Ingersoll, Gregory B.

    Volume Bragg gratings (VBGs) are important holographic optical elements in many spectral systems. Using multiple volume gratings, whether multiplexed or arranged sequentially, provides advantages to many types of systems in overall efficiency, dispersion performance, flexibility of design, etc. However, the use of multiple gratings---particularly when the gratings are multiplexed in a single holographic optical element (HOE)---is subject to inter-grating coupling effects that ultimately limit system performance. Analyzing these coupling effects requires a more complex mathematical model than the straightforward analysis of a single volume grating. We present a matrix-based algorithm for determining diffraction efficiencies of significant coupled waves in these multiplexed grating holographic optical elements (HOEs). Several carefully constructed experiments with spectrally multiplexed gratings in dichromated gelatin verify our conclusions. Applications of this theory to broad- and narrow-band systems are explored in detailed simulations. Broadband systems include spectrum splitters for diverse-bandgap photovoltaic (PV) cells. Volume Bragg gratings can serve as effective spectrum splitters, but the inherent dispersion of a VBG can be detrimental given a broad-spectrum input. The performance of a holographic spectrum splitter element can be improved by utilizing multiple volume gratings, each operating in a slightly different spectral band. However, care must be taken to avoid inter-grating coupling effects that limit ultimate performance. We explore broadband multi-grating holographic optical elements (HOEs) in sandwiched arrangements where individual single-grating HOEs are placed in series, and in multiplexed arrangements where multiple gratings are recorded in a single HOE. Particle swarm optimization (PSO) is used to tailor these systems to the solar spectrum taking into account both efficiency and dispersion. Both multiplexed and sandwiched two-grating systems

  18. Validation and implementation of the PowerPlex 16 BIO System STR multiplex for forensic casework.

    PubMed

    Greenspoon, Susan A; Ban, Jeffrey D; Pablo, Lourdes; Crouse, Cecelia A; Kist, Frank G; Tomsey, Chris S; Glessner, Alex L; Mihalacki, Lisa R; Long, Teresa M; Heidebrecht, Bruce J; Braunstein, Cathryn A; Freeman, David A; Soberalski, Carl; Nathan, Bruesehoff; Amin, Ashima S; Douglas, Elizabeth K; Schumm, James W

    2004-01-01

    The PowerPlex 16 BIO multiplex short tandem repeat (STR) system contains the 13 CODIS loci (FGA, TPOX, D8S1179, vWA, D18S51, D21S11, TH01, D3S1358, CSF1PO, D16S539, D7S820, D13S317, and DS5S818), plus two pentanucleotide repeat loci (Penta D and Penta E) and the sex-identifying locus. Amelogenin. The PowerPlex 16 BIO System is optimized for use with the Hitachi FMBIO gel imaging systems. A consortium of seven independent laboratories collaborated to perform the studies defined by the FBI standards for performing a developmental validation, including the evaluation of sample concordance, percent stutter determination, nonprobative casework, precision, sensitivity, mixture determination, effect of substrates, the impact of environmental insults, and species specificity. All samples tested for concordance were consistent except for one sample from the Virginia Division of Forensic Science database that displayed discordance at D13S317, a locus whose primer sequence was altered. Stutter values were comparable to those of other STR multiplex systems, the precision was comparable to other multiplexes analyzed by gel electrophoresis, the DNA profiles were unchanged by the substrate upon which the blood samples were placed, and the nonprobative casework samples re-typed for the PowerPlex 16 BIO System were consistent with previous typing results. When greater than 0.125 ng of DNA was placed into the PowerPlex 16 BIO System amplification reaction, a full profile was generated by all laboratories. The mixture study results were comparable to those reported for other multiplex systems, the environmental study demonstrated a loss of larger molecular weight loci when samples were incubated at elevated temperatures for a prolonged period of time, and the only notable cross species hybridization was observed with primate DNA samples. This extensive validation work performed demonstrates that the PowerPlex 16 BIO System provides STR data of a quality comparable with other Power

  19. The electronics readout and data acquisition system of the KM3NeT neutrino telescope node

    SciTech Connect

    Real, Diego [IFIC, Instituto de Física Corpuscular, CSIC-Universidad de Valencia, C Collaboration: KM3NeT Collaboration

    2014-11-18

    The KM3NeT neutrino telescope will be composed by tens of thousands of glass spheres, called Digital Optical Module (DOM), each of them containing 31 PMTs of small photocathode area (3'). The readout and data acquisition system of KM3NeT have to collect, treat and send to shore, in an economic way, the enormous amount of data produced by the photomultipliers and at the same time to provide time synchronization between each DOM at the level of 1 ns. It is described in the present article the Central Logic Board, that integrates the Time to Digital Converters and the White Rabbit protocol used for the DOM synchronization in a transparent way, the Power Board used in the DOM, the PMT base to readout the photomultipliers and the respective collecting boards, the so called Octopus Board.

  20. Intelligent two-loop time-division-multiplexing (TDM) optical frequency stabilization system for multichannel communications

    NASA Astrophysics Data System (ADS)

    Li, Aiguo; Wu, Deming; Xie, Linzhen

    1995-04-01

    A channel spacing frequency stabilization system for optical frequency-division-multiplexing communications is reported. Considering variable environmental condition and device aging effects, we have designed two loops in the system using a Fabry-perot interferometer as a frequency reference. One loop is a fine-tuning one, which is a time-division-multiplexing frequency stabilization scheme adjusting the driving currents of all the transmitter. The other loop is a rough-tuning one, which is a series of newly designed digital temperature controllers in which microprocessors and electrical oscillation circuits rather than Wheatstone bridge- circuits are used to detect the temperature error signal in order to reduce laser operating temperature dependence on the environmental conditions and there are RS-232 interfaces for communications with the first loop.

  1. Semiconductor laser with a birefringent external cavity for information systems with wavelength division multiplexing

    SciTech Connect

    Paranin, V D; Matyunin, S A; Tukmakov, K N

    2013-10-31

    The spectrum of a semiconductor laser with a birefringent external Gires – Tournois cavity is studied. The generation of two main laser modes corresponding to the ordinary and extraordinary wave resonances is found. It is shown that the radiation spectrum is controlled with a high energy efficiency without losses for spectral filtration. The possibility of using two-mode lasing in optical communication systems with wavelength division multiplexing is shown. (control of laser radiation parameters)

  2. Achieving fast timing performance with multiplexed SiPMs

    NASA Astrophysics Data System (ADS)

    Bieniosek, M. F.; Cates, J. W.; Levin, C. S.

    2016-04-01

    Using time of flight (ToF) measurements for positron emission tomography (PET) is an attractive avenue for increasing the signal to noise (SNR) ratio of PET images. However, achieving excellent time resolution required for high SNR gain using silicon photomultipliers (SiPM) requires many resource heavy high bandwidth readout channels. A method of multiplexing many SiPM signals into a single electronic channel would greatly simplify ToF PET systems. However, multiplexing SiPMs degrades time resolution because of added dark counts and signal shaping. In this work the relative contribution of dark counts and signal shaping to timing degradation is simulated and a baseline correction technique to mitigate the effect of multiplexing on the time resolution of analog SiPMs is simulated and experimentally verified. A charge sharing network for multiplexing is proposed and tested. Results show a full width at half maximum (FWHM) coincidence time resolution of 232+/- 2 ps for a single 3 mm  ×  3 mm  ×  20 mm LYSO scintillation crystals coupled to an array of sixteen 3 mm  ×  3 mm SiPMs that are multiplexed to a single timing channel (in addition to 4 position channels). A 4× 4 array of 3 mm  ×  3 mm  ×  20 mm LFS crystals showed an average FWHM coincidence time resolution of 278+/- 7 ps using the same timing scheme. All experiments were performed at room temperature with no thermal regulation. These results show that excellent time resolution for ToF can be achieved with a highly multiplexed analog SiPM readout.

  3. Development of Readout Electronics for POLARBEAR-2 Cosmic Microwave Background Experiment

    NASA Astrophysics Data System (ADS)

    Hattori, K.; Akiba, Y.; Arnold, K.; Barron, D.; Bender, A. N.; Cukierman, A.; de Haan, T.; Dobbs, M.; Elleflot, T.; Hasegawa, M.; Hazumi, M.; Holzapfel, W.; Hori, Y.; Keating, B.; Kusaka, A.; Lee, A.; Montgomery, J.; Rotermund, K.; Shirley, I.; Suzuki, A.; Whitehorn, N.

    2016-07-01

    The readout of transition-edge sensor (TES) bolometers with a large multiplexing factor is key for the next generation cosmic microwave background (CMB) experiment, Polarbear-2 (Suzuki in J Low Temp Phys 176:719, 2014), having 7588 TES bolometers. To enable the large arrays, we have been developing a readout system with a multiplexing factor of 40 in the frequency domain. Extending that architecture to 40 bolometers requires an increase in the bandwidth of the SQUID electronics, above 4 MHz. This paper focuses on cryogenic readout and shows how it affects cross talk and the responsivity of the TES bolometers. A series resistance, such as equivalent series resistance of capacitors for LC filters, leads to non-linear response of the bolometers. A wiring inductance modulates a voltage across the bolometers and causes cross talk. They should be controlled well to reduce systematic errors in CMB observations. We have been developing a cryogenic readout with a low series impedance and have tuned bolometers in the middle of their transition at a high frequency (>3 MHz).

  4. Advanced ACTPol Cryogenic Detector Arrays and Readout

    NASA Astrophysics Data System (ADS)

    Henderson, S. W.; Allison, R.; Austermann, J.; Baildon, T.; Battaglia, N.; Beall, J. A.; Becker, D.; De Bernardis, F.; Bond, J. R.; Calabrese, E.; Choi, S. K.; Coughlin, K. P.; Crowley, K. T.; Datta, R.; Devlin, M. J.; Duff, S. M.; Dunkley, J.; Dünner, R.; van Engelen, A.; Gallardo, P. A.; Grace, E.; Hasselfield, M.; Hills, F.; Hilton, G. C.; Hincks, A. D.; Hloẑek, R.; Ho, S. P.; Hubmayr, J.; Huffenberger, K.; Hughes, J. P.; Irwin, K. D.; Koopman, B. J.; Kosowsky, A. B.; Li, D.; McMahon, J.; Munson, C.; Nati, F.; Newburgh, L.; Niemack, M. D.; Niraula, P.; Page, L. A.; Pappas, C. G.; Salatino, M.; Schillaci, A.; Schmitt, B. L.; Sehgal, N.; Sherwin, B. D.; Sievers, J. L.; Simon, S. M.; Spergel, D. N.; Staggs, S. T.; Stevens, J. R.; Thornton, R.; Van Lanen, J.; Vavagiakis, E. M.; Ward, J. T.; Wollack, E. J.

    2016-08-01

    Advanced ACTPol is a polarization-sensitive upgrade for the 6 m aperture Atacama Cosmology Telescope, adding new frequencies and increasing sensitivity over the previous ACTPol receiver. In 2016, Advanced ACTPol will begin to map approximately half the sky in five frequency bands (28-230 GHz). Its maps of primary and secondary cosmic microwave background anisotropies—imaged in intensity and polarization at few arcminute-scale resolution—will enable precision cosmological constraints and also a wide array of cross-correlation science that probes the expansion history of the universe and the growth of structure via gravitational collapse. To accomplish these scientific goals, the Advanced ACTPol receiver will be a significant upgrade to the ACTPol receiver, including four new multichroic arrays of cryogenic, feedhorn-coupled AlMn transition edge sensor polarimeters (fabricated on 150 mm diameter wafers); a system of continuously rotating meta-material silicon half-wave plates; and a new multiplexing readout architecture which uses superconducting quantum interference devices and time division to achieve a 64-row multiplexing factor. Here we present the status and scientific goals of the Advanced ACTPol instrument, emphasizing the design and implementation of the Advanced ACTPol cryogenic detector arrays.

  5. Advanced ACTPol Cryogenic Detector Arrays and Readout

    NASA Technical Reports Server (NTRS)

    Henderson, S.W.; Allison, R.; Austermann, J.; Baildon, T.; Battaglia, N.; Beall, J. A.; Becker, D.; De Bernardis, F.; Bond, J. R.; Wollack, E. J.

    2016-01-01

    Advanced ACTPol is a polarization-sensitive upgrade for the 6 m aperture Atacama Cosmology Telescope, adding new frequencies and increasing sensitivity over the previous ACTPol receiver. In 2016, Advanced ACTPol will begin to map approximately half the sky in five frequency bands (28-230 GHz). Its maps of primary and secondary cosmic microwave background anisotropies-imaged in intensity and polarization at few arcminute-scale resolution-will enable precision cosmological constraints and also awide array of cross-correlation science that probes the expansion history of the universe and the growth of structure via gravitational collapse. To accomplish these scientific goals, the AdvancedACTPol receiver will be a significant upgrade to the ACTPol receiver, including four new multichroic arrays of cryogenic, feedhorn-coupled AlMn transition edge sensor polarimeters (fabricated on 150 mm diameter wafers); a system of continuously rotating meta-material silicon half-wave plates; and a new multiplexing readout architecture which uses superconducting quantum interference devices and time division to achieve a 64-row multiplexing factor. Here we present the status and scientific goals of the Advanced ACTPol instrument, emphasizing the design and implementation of the AdvancedACTPol cryogenic detector arrays.

  6. Optimization of a multiplex CRISPR/Cas system for use as an antiviral therapeutic.

    PubMed

    Kennedy, Edward M; Kornepati, Anand V R; Mefferd, Adam L; Marshall, Joy B; Tsai, Kevin; Bogerd, Hal P; Cullen, Bryan R

    2015-12-01

    RNA-guided endonucleases or CRISPR/Cas systems have been widely employed for gene engineering/DNA editing applications, and have recently been used against a variety of dsDNA viruses as a potential therapeutic. However, in vivo delivery to specific tissue reservoirs using adeno-associated virus (AAV) vectors is problematic due to the large coding requirement for the principal effector commonly used in these applications, Streptococcus pyogenes (Spy) Cas9. Here we describe design of a minimal CRISPR/Cas system that is capable of multiplexing and can be packaged into a single AAV vector. This system consists of the small Type II Cas9 protein from Staphylococcus aureus (Sau) driven by a truncated CMV promoter/enhancer, and flanked 3' by a poly(A) addition signal, as well as two sgRNA expression cassettes driven by either U6 or ∼70-bp tRNA-derived Pol III promoters. Specific protocols for construction of these AAV vector scaffolds, shuttle cloning of their contents into AAV and lentiviral backbones, and a quantitative luciferase assay capable of screening for optimal sgRNAs, are detailed. These protocols can facilitate construction of AAV vectors that have optimal multiplexed sgRNA expression and function. These will have potential utility in multiplex applications, including in antiviral therapy in tissues chronically infected with a pathogenic DNA virus. PMID:26291065

  7. Developments in Time-Division Multiplexing of X-ray Transition-Edge Sensors

    NASA Astrophysics Data System (ADS)

    Doriese, W. B.; Morgan, K. M.; Bennett, D. A.; Denison, E. V.; Fitzgerald, C. P.; Fowler, J. W.; Gard, J. D.; Hays-Wehle, J. P.; Hilton, G. C.; Irwin, K. D.; Joe, Y. I.; Mates, J. A. B.; O'Neil, G. C.; Reintsema, C. D.; Robbins, N. O.; Schmidt, D. R.; Swetz, D. S.; Tatsuno, H.; Vale, L. R.; Ullom, J. N.

    2016-07-01

    Time-division multiplexing (TDM) is a mature scheme for the readout of arrays of transition-edge sensors (TESs). TDM is based on superconducting-quantum-interference-device (SQUID) current amplifiers. Multiple spectrometers based on gamma-ray and X-ray microcalorimeters have been operated with TDM readout, each at the scale of 200 sensors per spectrometer, as have several astronomical cameras with thousands of sub-mm or microwave bolometers. Here we present the details of two different versions of our TDM system designed to read out X-ray TESs. The first has been field-deployed in two 160-sensor (8 columns × 20 rows) spectrometers and four 240-sensor (8 columns × 30 rows) spectrometers. It has a three-SQUID-stage architecture, switches rows every 320 ns, and has total readout noise of 0.41 μ Φ 0 / surd Hz. The second, which is presently under development, has a two-SQUID-stage architecture, switches rows every 160 ns, and has total readout noise of 0.19 μ Φ 0 / surd Hz. Both quoted noise values are non-multiplexed and referred to the first-stage SQUID. In a demonstration of this new architecture, a multiplexed 1-column × 32-row array of NIST TESs achieved average energy resolution of 2.55± 0.01 eV at 6 keV.

  8. A DSP-based readout and online processing system for a new focal-plane polarimeter at AGOR

    NASA Astrophysics Data System (ADS)

    Hagemann, M.; Bassini, R.; van den Berg, A. M.; Ellinghaus, F.; Frekers, D.; Hannen, V. M.; Häupke, T.; Heyse, J.; Jacobs, E.; Kirsch, M.; Krüsemann, B.; Rakers, S.; Sohlbach, H.; Wörtche, H. J.

    1999-11-01

    A Focal-Plane Polarimeter (FPP) for the large acceptance Big-Bite Spectrometer (BBS) at AGOR using a novel readout architecture has been commissioned at the KVI Groningen. The instrument is optimized for medium-energy polarized proton scattering near or at 0°. For the handling of the high counting rates at extreme forward angles and for the suppression of small-angle scattering in the graphite analyzer, a high-performance data processing DSP system connecting to the LeCroy FERA and PCOS ECL bus architecture has been made operational and tested successfully. Details of the system and the functions of the various electronic components are described.

  9. Development of a Microwave SQUID-Multiplexed TES Array for MUSTANG-2

    NASA Astrophysics Data System (ADS)

    Stanchfield, S. M.; Ade, P. A. R.; Aguirre, J.; Brevik, J. A.; Cho, H. M.; Datta, R.; Devlin, M. J.; Dicker, S. R.; Dober, B.; Egan, D.; Ford, P.; Hilton, G.; Hubmayr, J.; Irwin, K. D.; Marganian, P.; Mason, B. S.; Mates, J. A. B.; McMahon, J.; Mello, M.; Mroczkowski, T.; Romero, C.; Tucker, C.; Vale, L.; White, S.; Whitehead, M.; Young, A. H.

    2016-07-01

    MUSTANG-2 is a 90 GHz feedhorn-coupled, microwave SQUID-multiplexed TES bolometer array in the final stages of development for operation on the 100-m Robert C. Byrd Green Bank Telescope. We present the camera design and report the performance during the first season of observation, in which 64 of the available 215 pixels in the focal plane were populated. We highlight the microwave multiplexing readout technology, which is envisioned as a path to read out the next generation of large pixel-count cryogenic focal planes. In this regard, MUSTANG2 is a pathfinder for this multiplexing technology. We present noise spectra which show no detector noise degradation when read out with microwave SQUID multiplexing, and we present first light images of Jupiter and M87, which demonstrate the end-to-end system performance.

  10. A novel MUX/DEMUX based on few-mode FBG for mode division multiplexing system

    NASA Astrophysics Data System (ADS)

    Han, Yueyu; Hu, Guijun

    2016-05-01

    In this paper, a novel mode multiplexer/demultiplexer (MUX/DEMUX) based on few-mode fiber Bragg gratings (FBG) has been proposed. The principle of the MUX/DEMUX based on few-mode FBG has been described in detail, and crosstalk of better than -20 dB is obtained experimentally. Then a 2×2 division multiplexing (MDM) system has been established with the MUX/DEMUX we proposed. The transmission experiment of 2×10 Gbps PRBS has been achieved successfully, which are carried by LP01 mode and LP11 mode, respectively. When the receiver sensitivity is greater than -14 dB m and -10 dB m, the BER can both reach 10-3 for B2B and 10 km transmission, respectively.

  11. A 16-Channel Distributed-Feedback Laser Array with a Monolithic Integrated Arrayed Waveguide Grating Multiplexer for a Wavelength Division Multiplex-Passive Optical Network System Network

    NASA Astrophysics Data System (ADS)

    Zhao, Jian-Yi; Chen, Xin; Zhou, Ning; Huang, Xiao-Dong; Cao, Ming-De; Liu, Wen

    2014-07-01

    A 16-channel distributed-feedback (DFB) laser array with a monolithic integrated arrayed waveguide grating multiplexer for a wavelength division multiplex-passive optical network system is fabricated by using the butt-joint metal organic chemical vapor deposition technology and nanoimpirnt technology. The results show that the threshold current is about 20-30 mA at 25°C. The DFB laser side output power is about 16 mW with a 150 mA injection current. The lasing wavelength is from 1550 nm to 1575 nm covering a more than 25 nm range with 200 GHz channel space. A more than 55 dB sidemode suppression ratio is obtained.

  12. Unyvero i60 implant and tissue infection (ITI) multiplex PCR system in diagnosing periprosthetic joint infection.

    PubMed

    Hischebeth, Gunnar T R; Randau, Thomas M; Buhr, Johanna K; Wimmer, Matthias D; Hoerauf, Achim; Molitor, Ernst; Bekeredjian-Ding, Isabelle; Gravius, Sascha

    2016-02-01

    Periprosthetic joint infection (PJI) is one of the most challenging complications in orthopedic surgery. In cases of suspected periprosthetic joint infection several diagnostic methods are available. In this study we investigated the performance of the newly available Unyvero i60 implant and tissue infection (ITI) multiplex PCR System. 62 specimens from 31 patients with suspected PJI or aseptic loosening of a painful joint arthoplasty were included in this study. Besides the established diagnostic procedures we included a commercial multiplex PCR detection system for diagnosis of PJI. The PCR results obtained from analysis of sonication and synovial fluids (62 specimens) showed a sensitivity of 66.7%, a specificity of 100%, a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 68.4% when compared to cultural methods. Notably, cultures from sonication fluid displayed a sensitivity of 88.9%, a specificity of 61.5%, a PPV of 76.2% and a NPV of 80.0% when compared to tissue cultures. In conclusion, multiplex PCR is an additional - rapid - method for diagnosing PJI. Positive results with the PCR assay used in this study were always confirmed by subsequent matching culture positivity. However, apart from the time saved the nucleic acid amplification technique did not yield additional information than that obtained from microbiological cultures. PMID:26689142

  13. Development of a beam test telescope based on the Alibava readout system

    NASA Astrophysics Data System (ADS)

    Marco-Hernández, R.

    2011-01-01

    A telescope for a beam test have been developed as a result of a collaboration among the University of Liverpool, Centro Nacional de Microelectrónica (CNM) of Barcelona and Instituto de Física Corpuscular (IFIC) of Valencia. This system is intended to carry out both analogue charge collection and spatial resolution measurements with different types of microstrip or pixel silicon detectors in a beam test environment. The telescope has four XY measurement as well as trigger planes (XYT board) and it can accommodate up to twelve devices under test (DUT board). The DUT board uses two Beetle ASICs for the readout of chilled silicon detectors. The board could operate in a self-triggering mode. The board features a temperature sensor and it can be mounted on a rotary stage. A peltier element is used for cooling the DUT. Each XYT board measures the track space points using two silicon strip detectors connected to two Beetle ASICs. It can also trigger on the particle tracks in the beam test. The board includes a CPLD which allows for the synchronization of the trigger signal to a common clock frequency, delaying and implementing coincidence with other XYT boards. An Alibava mother board is used to read out and to control each XYT/DUT board from a common trigger signal and a common clock signal. The Alibava board has a TDC on board to have a time stamp of each trigger. The data collected by each Alibava board is sent to a master card by means of a local data/address bus following a custom digital protocol. The master board distributes the trigger, clock and reset signals. It also merges the data streams from up to sixteen Alibava boards. The board has also a test channel for testing in a standard mode a XYT or DUT board. This board is implemented with a Xilinx development board and a custom patch board. The master board is connected with the DAQ software via 100M Ethernet. Track based alignment software has also been developed for the data obtained with the DAQ software.

  14. Common Readout Unit (CRU) - A new readout architecture for the ALICE experiment

    NASA Astrophysics Data System (ADS)

    Mitra, J.; Khan, S. A.; Mukherjee, S.; Paul, R.

    2016-03-01

    The ALICE experiment at the CERN Large Hadron Collider (LHC) is presently going for a major upgrade in order to fully exploit the scientific potential of the upcoming high luminosity run, scheduled to start in the year 2021. The high interaction rate and the large event size will result in an experimental data flow of about 1 TB/s from the detectors, which need to be processed before sending to the online computing system and data storage. This processing is done in a dedicated Common Readout Unit (CRU), proposed for data aggregation, trigger and timing distribution and control moderation. It act as common interface between sub-detector electronic systems, computing system and trigger processors. The interface links include GBT, TTC-PON and PCIe. GBT (Gigabit transceiver) is used for detector data payload transmission and fixed latency path for trigger distribution between CRU and detector readout electronics. TTC-PON (Timing, Trigger and Control via Passive Optical Network) is employed for time multiplex trigger distribution between CRU and Central Trigger Processor (CTP). PCIe (Peripheral Component Interconnect Express) is the high-speed serial computer expansion bus standard for bulk data transport between CRU boards and processors. In this article, we give an overview of CRU architecture in ALICE, discuss the different interfaces, along with the firmware design and implementation of CRU on the LHCb PCIe40 board.

  15. Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections.

    PubMed

    Prost, Sandrine; Kishen, Ria E B; Kluth, David C; Bellamy, Christopher O C

    2016-01-01

    The recent availability of novel dyes and alternative light sources to facilitate complex tissue immunofluorescence studies such as multiplex labelling has not been matched by reports critically evaluating the considerations and relative benefits of these new tools, particularly in combination. Product information is often limited to wavelengths used for older fluorophores (FITC, TRITC & corresponding Alexa dyes family). Consequently, novel agents such as Quantum dots are not widely appreciated or used, despite highly favourable properties including extremely bright emission, stability and potentially reduced tissue autofluorescence at the excitation wavelength. Using spectral analysis, we report here a detailed critical appraisal and comparative evaluation of different light sources and fluorophores in multiplex immunofluorescence of clinical biopsy sections. The comparison includes mercury light, metal halide and 3 different LED-based systems, using 7 Qdots (525, 565, 585, 605, 625, 705), Cy3 and Cy5. We discuss the considerations relevant to achieving the best combination of light source and fluorophore for accurate multiplex fluorescence quantitation. We highlight practical limitations and confounders to quantitation with filter-based approaches. PMID:27632367

  16. Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections

    PubMed Central

    Kishen, Ria E. B.; Kluth, David C.; Bellamy, Christopher O. C.

    2016-01-01

    The recent availability of novel dyes and alternative light sources to facilitate complex tissue immunofluorescence studies such as multiplex labelling has not been matched by reports critically evaluating the considerations and relative benefits of these new tools, particularly in combination. Product information is often limited to wavelengths used for older fluorophores (FITC, TRITC & corresponding Alexa dyes family). Consequently, novel agents such as Quantum dots are not widely appreciated or used, despite highly favourable properties including extremely bright emission, stability and potentially reduced tissue autofluorescence at the excitation wavelength. Using spectral analysis, we report here a detailed critical appraisal and comparative evaluation of different light sources and fluorophores in multiplex immunofluorescence of clinical biopsy sections. The comparison includes mercury light, metal halide and 3 different LED-based systems, using 7 Qdots (525, 565, 585, 605, 625, 705), Cy3 and Cy5. We discuss the considerations relevant to achieving the best combination of light source and fluorophore for accurate multiplex fluorescence quantitation. We highlight practical limitations and confounders to quantitation with filter-based approaches. PMID:27632367

  17. Charge Identification of Highly Ionizing Particles in Desensitized Nuclear Emulsion Using High Speed Read-Out System

    SciTech Connect

    Toshito, T.; Kodama, K.; Yusa, K.; Ozaki, M.; Amako, K.; Kameoka, S.; Murakami, K.; Sasaki, T.; Aoki, S.; Ban, T.; Fukuda, T.; Naganawa, N.; Nakamura, T.; Natsume, M.; Niwa, K.; Takahashi, S.; Kanazawa, M.; Kanematsu, N.; Komori, M.; Sato, S.; Asai, M.; /Nagoya U. /Aichi U. of Education /Gunma U., Maebashi /JAXA, Sagamihara /KEK, Tsukuba /Kobe U. /Chiba, Natl. Inst. Rad. Sci. /SLAC /Toho U.

    2006-05-10

    We performed an experimental study of charge identification of heavy ions from helium to carbon having energy of about 290 MeV/u using an emulsion chamber. Emulsion was desensitized by means of forced fading (refreshing) to expand a dynamic range of response to highly charged particles. For the track reconstruction and charge identification, the fully automated high speed emulsion read-out system, which was originally developed for identifying minimum ionizing particles, was used without any modification. Clear track by track charge identification up to Z=6 was demonstrated. The refreshing technique has proved to be a powerful technique to expand response of emulsion film to highly ionizing particles.

  18. A programmable energy efficient readout chip for a multiparameter highly integrated implantable biosensor system

    NASA Astrophysics Data System (ADS)

    Nawito, M.; Richter, H.; Stett, A.; Burghartz, J. N.

    2015-11-01

    In this work an Application Specific Integrated Circuit (ASIC) for an implantable electrochemical biosensor system (SMART implant, Stett et al., 2014) is presented. The ASIC drives the measurement electrodes and performs amperometric measurements for determining the oxygen concentration, potentiometric measurements for evaluating the pH-level as well as temperature measurements. A 10-bit pipeline analog to digital (ADC) is used to digitize the acquired analog samples and is implemented as a single stage to reduce power consumption and chip area. For pH measurements, an offset subtraction technique is employed to raise the resolution to 12-bits. Charge integration is utilized for oxygen and temperature measurements with the capability to cover current ranges between 30 nA and 1 μA. In order to achieve good performance over a wide range of supply and process variations, internal reference voltages are generated from a programmable band-gap regulated circuit and biasing currents are supplied from a wide-range bootstrap current reference. To accommodate the limited available electrical power, all components are designed for low power operation. Also a sequential operation approach is applied, in which essential circuit building blocks are time multiplexed between different measurement types. All measurement sequences and parameters are programmable and can be adjusted for different tissues and media. The chip communicates with external unites through a full duplex two-wire Serial Peripheral Interface (SPI), which receives operational instructions and at the same time outputs the internally stored measurement data. The circuit has been fabricated in a standard 0.5-μm CMOS process and operates on a supply as low as 2.7 V. Measurement results show good performance and agree with circuit simulation. It consumes a maximum of 500 μA DC current and is clocked between 500 kHz and 4 MHz according to the measurement parameters. Measurement results of the on-chip ADC show a

  19. 360-degree dense multiview image acquisition system using time multiplexing

    NASA Astrophysics Data System (ADS)

    Yendo, Tomohiro; Fujii, Toshiaki; Panahpour Tehrani, Mehrdad; Tanimoto, Masayuki

    2010-02-01

    A novel 360-degree 3D image acquisition system that captures multi-view images with narrow view interval is proposed. The system consists of a scanning optics system and a high-speed camera. The scanning optics system is composed of a double-parabolic mirror shell and a rotating flat mirror tilted at 45 degrees to the horizontal plane. The mirror shell produces a real image of an object that is placed at the bottom of the shell. The mirror shell is modified from usual system which is used as 3D illusion toy so that the real image can be captured from right horizontal viewing direction. The rotating mirror in the real image reflects the image to the camera-axis direction. The reflected image observed from the camera varies according to the angle of the rotating mirror. This means that the camera can capture the object from various viewing directions that are determined by the angle of the rotating mirror. To acquire the time-varying reflected images, we use a high-speed camera that is synchronized with the angle of the rotating mirror. We have used a high-speed camera which resolution is 256×256 and the maximum frame rate is 10000fps at the resolution. Rotating speed of the tilted flat mirror is about 27 rev./sec. The number of views is 360. The focus length of parabolic mirrors is 73mm and diameter is 360mm. Objects which length is less than about 30mm can be acquired. Captured images are compensated rotation and distortion caused by double-parabolic mirror system, and reproduced as 3D moving images by Seelinder display.

  20. ADVANCED READOUT ELECTRONICS FOR MULTIELEMENT CdZnTe SENSORS.

    SciTech Connect

    DE GERONIMO,G.; O CONNOR,P.; KANDASAMY,A.; GROSHOLZ,J.

    2002-07-08

    A generation of high performance front-end and read-out ASICs customized for highly segmented CdZnTe sensors is presented. The ASICs, developed in a multi-year effort at Brookhaven National Laboratory, are targeted to a wide range of applications including medical, safeguards/security, industrial, research, and spectroscopy. The front-end multichannel ASICs provide high accuracy low noise preamplification and filtering of signals, with versions for small and large area CdZnTe elements. They implement a high order unipolar or bipolar shaper, an innovative low noise continuous reset system with self-adapting capability to the wide range of detector leakage currents, a new system for stabilizing the output baseline and high output driving capability. The general-purpose versions include programmable gain and peaking time. The read-out multichannel ASICs provide fully data driven high accuracy amplitude and time measurements, multiplexing and time domain derandomization of the shaped pulses. They implement a fast arbitration scheme and an array of innovative two-phase offset-free rail-to-rail analog peak detectors for buffering and absorption of input rate fluctuations, thus greatly relaxing the rate requirement on the external ADC. Pulse amplitude, hit timing, pulse risetime, and channel address per processed pulse are available at the output in correspondence of an external readout request. Prototype chips have been fabricated in 0.5 and 0.35 {micro}m CMOS and tested. Design concepts and experimental results are discussed.

  1. A handheld flow genetic analysis system (FGAS): towards rapid, sensitive, quantitative and multiplex molecular diagnosis at the point-of-care level.

    PubMed

    Shu, Bowen; Zhang, Chunsun; Xing, Da

    2015-06-21

    A handheld flow genetic analysis system (FGAS) is proposed for rapid, sensitive, multiplex and real-time quantification of nucleic acids at the point-of-care (POC) level. The FGAS includes a helical thermal-gradient microreactor and a microflow actuator, as well as control circuitry for temperature, fluid and power management, and smartphone fluorescence imaging. All of these features are integrated into a field-portable and easy-to-use molecular diagnostic platform powered by lithium batteries. Due to the unique design of the microreactor, not only steady temperatures for denaturation and annealing/extension but also a linear thermal gradient for spatial high-resolution melting can be achieved through simply maintaining a single heater at constant temperature. The smartphone fluorescence imaging system has a wide field of view that captures all PCR channels of the microreactor in a single snapshot without the need for any mechanical scanning. By these designs, the FGAS enables real-time monitoring of the temporal and spatial fluorescence signatures of amplicons during continuous-flow amplification. On the current FGAS, visual detection of as little as 10 copies per μL of genomic DNA of Salmonella enterica was achieved in 15 min, with real-time quantitative detection of the DNA over 6 orders of magnitude concentration from 10(6) to 10(1) copies per μL also completed in 7.5-15 min. In addition, multiple pathogenic DNA targets could be simultaneously discriminated with direct bar-chart readout or multiplex spatial melting in serial flow. We anticipate that the FGAS has great potential to become a next-generation gene analyzer for POC molecular diagnostics. PMID:25953325

  2. A multiplexed immunoassay system based upon reciprocating centrifugal microfluidics

    NASA Astrophysics Data System (ADS)

    Noroozi, Zahra; Kido, Horacio; Peytavi, Régis; Nakajima-Sasaki, Rie; Jasinskas, Algimantas; Micic, Miodrag; Felgner, Philip L.; Madou, Marc J.

    2011-06-01

    A novel, centrifugal disk-based micro-total analysis system (μTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. The described reciprocating mechanism results in a reduction in processing time and reagent consumption by one order of magnitude.

  3. Installation of a multiplexed Raman system in a chemical process.

    PubMed

    Weyer, Lois G; Yee, Dupon; Pearson, Gary I; Wade, John

    2004-12-01

    A 785 nm laser dispersive Raman system was installed in a hazardous chemical production plant to monitor two reactors, a still, and a holding tank with measurement directly through sight glasses. Compositional information for several raw materials, intermediates, and products was obtained using partial least squares (PLS) calibrations. Discriminate analysis was used to exclude extremely poor spectra while including usable ones. Derivative/standard normal variate (SNV) treatment was found to be effective for correcting for background fluorescence and for large differences in spectral intensity. Final regression equations incorporated temperature fluctuations, occasional fluorescence, some sunlight effects, large intensity variations, and large compositional changes. Various installation problems were solved via data treatment or mechanical changes. The Raman system provides information for control of the process, resulting in cost savings and improved safety.

  4. Evaluation of some wide dynamic range readout systems for photoelectric integrating spectrometers

    NASA Technical Reports Server (NTRS)

    Franklin, M. L.; Gordon, W. A.

    1971-01-01

    Five types of electronic circuits commonly used for readout of currents from photomultiplier tubes were evaluated. This evaluation was conducted to define the experimental limits of achieving the widest possible dynamic range of light detection and concentration measurements in spectrochemical analysis. The circuits evaluated included the photon-counting technique and four dc circuits. These circuits displayed a dynamic range from 3 to about 4.5 decades within a repeatability criterion of 1 percent, or better. The maximum dynamic range allowed by the photomultiplier tube in this application was estimated to be about 6 decades.

  5. Development of an Optical Read-Out System for the LISA/NGO Gravitational Reference Sensor: A Status Report

    NASA Astrophysics Data System (ADS)

    Di Fiore, L.; De Rosa, R.; Garufi, F.; Grado, A.; Milano, L.; Spagnuolo, V.; Russano, G.

    2013-01-01

    The LISA group in Napoli is working on the development of an Optical Read-Out (ORO) system, based on optical levers and position sensitive detectors, for the LISA gravitational reference sensor. ORO is not meant as an alternative, but as an addition, to capacitive readout, that is the reference solution for LISA/NGO and will be tested on flight by LISA-Pathfinder. The main goal is the introduction of some redundancy with consequent mission risk mitigation. Furthermore, the ORO system is more sensitive than the capacitive one and its usage would allow a significant relaxation of the specifications on cross-couplings in the drag free control loops. The reliability of the proposed ORO device and the fulfilment of the sensitivity requirements have been already demonstrated in bench-top measurements and tests with the four mass torsion pendulum developed in Trento as a ground testing facility for LISA-Pathfinder and LISA hardware. In this paper we report on the present status of this activity presenting the last results and perspectives on some relevant aspects. 1) System design, measured sensitivity and noise characterization. 2) Possible layouts for integration in LISA/NGO and bench-top tests on real scale prototypes. 3) Search for space compatible components and preliminary tests. We will also discuss next steps in view of a possible application in LISA/NGO.

  6. Multiplex APLP System for High-Resolution Haplogrouping of Extremely Degraded East-Asian Mitochondrial DNAs

    PubMed Central

    Kakuda, Tsuneo; Shojo, Hideki; Tanaka, Mayumi; Nambiar, Phrabhakaran; Minaguchi, Kiyoshi; Umetsu, Kazuo; Adachi, Noboru

    2016-01-01

    Mitochondrial DNA (mtDNA) serves as a powerful tool for exploring matrilineal phylogeographic ancestry, as well as for analyzing highly degraded samples, because of its polymorphic nature and high copy numbers per cell. The recent advent of complete mitochondrial genome sequencing has led to improved techniques for phylogenetic analyses based on mtDNA, and many multiplex genotyping methods have been developed for the hierarchical analysis of phylogenetically important mutations. However, few high-resolution multiplex genotyping systems for analyzing East-Asian mtDNA can be applied to extremely degraded samples. Here, we present a multiplex system for analyzing mitochondrial single nucleotide polymorphisms (mtSNPs), which relies on a novel amplified product-length polymorphisms (APLP) method that uses inosine-flapped primers and is specifically designed for the detailed haplogrouping of extremely degraded East-Asian mtDNAs. We used fourteen 6-plex polymerase chain reactions (PCRs) and subsequent electrophoresis to examine 81 haplogroup-defining SNPs and 3 insertion/deletion sites, and we were able to securely assign the studied mtDNAs to relevant haplogroups. Our system requires only 1×10−13 g (100 fg) of crude DNA to obtain a full profile. Owing to its small amplicon size (<110 bp), this new APLP system was successfully applied to extremely degraded samples for which direct sequencing of hypervariable segments using mini-primer sets was unsuccessful, and proved to be more robust than conventional APLP analysis. Thus, our new APLP system is effective for retrieving reliable data from extremely degraded East-Asian mtDNAs. PMID:27355212

  7. Multiplex APLP System for High-Resolution Haplogrouping of Extremely Degraded East-Asian Mitochondrial DNAs.

    PubMed

    Kakuda, Tsuneo; Shojo, Hideki; Tanaka, Mayumi; Nambiar, Phrabhakaran; Minaguchi, Kiyoshi; Umetsu, Kazuo; Adachi, Noboru

    2016-01-01

    Mitochondrial DNA (mtDNA) serves as a powerful tool for exploring matrilineal phylogeographic ancestry, as well as for analyzing highly degraded samples, because of its polymorphic nature and high copy numbers per cell. The recent advent of complete mitochondrial genome sequencing has led to improved techniques for phylogenetic analyses based on mtDNA, and many multiplex genotyping methods have been developed for the hierarchical analysis of phylogenetically important mutations. However, few high-resolution multiplex genotyping systems for analyzing East-Asian mtDNA can be applied to extremely degraded samples. Here, we present a multiplex system for analyzing mitochondrial single nucleotide polymorphisms (mtSNPs), which relies on a novel amplified product-length polymorphisms (APLP) method that uses inosine-flapped primers and is specifically designed for the detailed haplogrouping of extremely degraded East-Asian mtDNAs. We used fourteen 6-plex polymerase chain reactions (PCRs) and subsequent electrophoresis to examine 81 haplogroup-defining SNPs and 3 insertion/deletion sites, and we were able to securely assign the studied mtDNAs to relevant haplogroups. Our system requires only 1×10-13 g (100 fg) of crude DNA to obtain a full profile. Owing to its small amplicon size (<110 bp), this new APLP system was successfully applied to extremely degraded samples for which direct sequencing of hypervariable segments using mini-primer sets was unsuccessful, and proved to be more robust than conventional APLP analysis. Thus, our new APLP system is effective for retrieving reliable data from extremely degraded East-Asian mtDNAs. PMID:27355212

  8. Robustness analysis of an intensity modulated fiber-optic position sensor with an image sensor readout system.

    PubMed

    Jason, Johan; Nilsson, Hans-Erik; Arvidsson, Bertil; Larsson, Anders

    2013-06-01

    An intensity modulated fiber-optic position sensor, based on a fiber-to-bundle coupling and a readout system using a CMOS image camera together with fast routines for position extraction and calibration, is presented and analyzed. The proposed system eliminates alignment issues otherwise associated with coupling-based fiber-optic sensors, still keeping the sensing point free from detector electronics. In this study the robustness of the system is characterized through simulations of the system performance, and the outcome is compared with experimental results. It is shown that knowledge of the shape of the coupled power distribution is the single most important factor for high performance of the system. Further it is experimentally shown that the position extraction error can be improved down to the theoretical limit by employing a modulation function model well fitted to the real coupled power distribution.

  9. Advanced Spatial-Division Multiplexed Measurement Systems Propositions-From Telecommunication to Sensing Applications: A Review.

    PubMed

    Weng, Yi; Ip, Ezra; Pan, Zhongqi; Wang, Ting

    2016-01-01

    The concepts of spatial-division multiplexing (SDM) technology were first proposed in the telecommunications industry as an indispensable solution to reduce the cost-per-bit of optical fiber transmission. Recently, such spatial channels and modes have been applied in optical sensing applications where the returned echo is analyzed for the collection of essential environmental information. The key advantages of implementing SDM techniques in optical measurement systems include the multi-parameter discriminative capability and accuracy improvement. In this paper, to help readers without a telecommunication background better understand how the SDM-based sensing systems can be incorporated, the crucial components of SDM techniques, such as laser beam shaping, mode generation and conversion, multimode or multicore elements using special fibers and multiplexers are introduced, along with the recent developments in SDM amplifiers, opto-electronic sources and detection units of sensing systems. The examples of SDM-based sensing systems not only include Brillouin optical time-domain reflectometry or Brillouin optical time-domain analysis (BOTDR/BOTDA) using few-mode fibers (FMF) and the multicore fiber (MCF) based integrated fiber Bragg grating (FBG) sensors, but also involve the widely used components with their whole information used in the full multimode constructions, such as the whispering gallery modes for fiber profiling and chemical species measurements, the screw/twisted modes for examining water quality, as well as the optical beam shaping to improve cantilever deflection measurements. Besides, the various applications of SDM sensors, the cost efficiency issue, as well as how these complex mode multiplexing techniques might improve the standard fiber-optic sensor approaches using single-mode fibers (SMF) and photonic crystal fibers (PCF) have also been summarized. Finally, we conclude with a prospective outlook for the opportunities and challenges of SDM

  10. Advanced Spatial-Division Multiplexed Measurement Systems Propositions—From Telecommunication to Sensing Applications: A Review

    PubMed Central

    Weng, Yi; Ip, Ezra; Pan, Zhongqi; Wang, Ting

    2016-01-01

    The concepts of spatial-division multiplexing (SDM) technology were first proposed in the telecommunications industry as an indispensable solution to reduce the cost-per-bit of optical fiber transmission. Recently, such spatial channels and modes have been applied in optical sensing applications where the returned echo is analyzed for the collection of essential environmental information. The key advantages of implementing SDM techniques in optical measurement systems include the multi-parameter discriminative capability and accuracy improvement. In this paper, to help readers without a telecommunication background better understand how the SDM-based sensing systems can be incorporated, the crucial components of SDM techniques, such as laser beam shaping, mode generation and conversion, multimode or multicore elements using special fibers and multiplexers are introduced, along with the recent developments in SDM amplifiers, opto-electronic sources and detection units of sensing systems. The examples of SDM-based sensing systems not only include Brillouin optical time-domain reflectometry or Brillouin optical time-domain analysis (BOTDR/BOTDA) using few-mode fibers (FMF) and the multicore fiber (MCF) based integrated fiber Bragg grating (FBG) sensors, but also involve the widely used components with their whole information used in the full multimode constructions, such as the whispering gallery modes for fiber profiling and chemical species measurements, the screw/twisted modes for examining water quality, as well as the optical beam shaping to improve cantilever deflection measurements. Besides, the various applications of SDM sensors, the cost efficiency issue, as well as how these complex mode multiplexing techniques might improve the standard fiber-optic sensor approaches using single-mode fibers (SMF) and photonic crystal fibers (PCF) have also been summarized. Finally, we conclude with a prospective outlook for the opportunities and challenges of SDM

  11. Advanced Spatial-Division Multiplexed Measurement Systems Propositions-From Telecommunication to Sensing Applications: A Review.

    PubMed

    Weng, Yi; Ip, Ezra; Pan, Zhongqi; Wang, Ting

    2016-01-01

    The concepts of spatial-division multiplexing (SDM) technology were first proposed in the telecommunications industry as an indispensable solution to reduce the cost-per-bit of optical fiber transmission. Recently, such spatial channels and modes have been applied in optical sensing applications where the returned echo is analyzed for the collection of essential environmental information. The key advantages of implementing SDM techniques in optical measurement systems include the multi-parameter discriminative capability and accuracy improvement. In this paper, to help readers without a telecommunication background better understand how the SDM-based sensing systems can be incorporated, the crucial components of SDM techniques, such as laser beam shaping, mode generation and conversion, multimode or multicore elements using special fibers and multiplexers are introduced, along with the recent developments in SDM amplifiers, opto-electronic sources and detection units of sensing systems. The examples of SDM-based sensing systems not only include Brillouin optical time-domain reflectometry or Brillouin optical time-domain analysis (BOTDR/BOTDA) using few-mode fibers (FMF) and the multicore fiber (MCF) based integrated fiber Bragg grating (FBG) sensors, but also involve the widely used components with their whole information used in the full multimode constructions, such as the whispering gallery modes for fiber profiling and chemical species measurements, the screw/twisted modes for examining water quality, as well as the optical beam shaping to improve cantilever deflection measurements. Besides, the various applications of SDM sensors, the cost efficiency issue, as well as how these complex mode multiplexing techniques might improve the standard fiber-optic sensor approaches using single-mode fibers (SMF) and photonic crystal fibers (PCF) have also been summarized. Finally, we conclude with a prospective outlook for the opportunities and challenges of SDM

  12. Recent Results of a New Microwave SQUID Multiplexer

    NASA Technical Reports Server (NTRS)

    Hahn, Inseob; Limketkai, B.; Bumble, B.; LeDuc, H. G.

    2007-01-01

    We are developing a proof-of-concept microwave SQUID multiplexer containing four SQUIDs coupled to GHz frequency resonant circuits and fed with a single microwave readout line. The resonators are half-wave coplanar waveguide sections and are similar to the structures used for the microwave kinetic inductance detectors developed in our group. Optimal values for the interdigital gap capacitors were determined to maximize the sensitivity of the transmitted and reflected microwave signal with respect to changes in the dynamic resistance of the SQUID. The dc current-bias line for the SQUID has an in-line inductive high frequency filter to minimize coupling between the bias line and resonator. A high frequency modulation scheme is proposed to eliminate the need for individual flux biasing of the SQUIDs, which extends the dynamic range of the readout. In this scheme a common modulation signal is imposed on each SQUID and the received signal is demodulated at one and two times the modulation frequency to maintain sensitivity at any flux state. We present the recent results of the microwave SQUID multiplexer system operating at a readout frequency range of 10 - 11GHz.

  13. A true singles list-mode data acquisition system for a small animal PET scanner with independent crystal readout.

    PubMed

    McElroy, D P; Hoose, M; Pimpl, W; Spanoudaki, V; Schüler, T; Ziegler, S I

    2005-07-21

    We present a unique data acquisition system designed to read out signals from the MADPET-II small animal LSO-APD PET tomograph. The scanner consists of 36 independent detector modules arranged in a dual-radial layer ring (phi 71 mm). Each module contains a 4 x 8 array of optically isolated, 2 x 2 mm LSO crystals, coupled one-to-one to a 32 channel APD. To take full advantage of the detector geometry, signals from each crystal are individually processed without any data reduction. This is realized using custom designed mixed-signal ASICs for analogue signal processing, and FPGAs to control the digitization of analogue signals and subsequent multiplexing. Analogue to digital converters (ADCs) digitize the signal peak height, time to digital converters (TDCs) time stamp each event relative to a system clock and two 32 bit words containing the energy, time and position information for each singles event are multiplexed through three FIFO stages before being written to disk via gigabit Ethernet. Every singles event is processed and stored in list-mode format, and coincidences are sorted post-acquisition in software. The 1152 channel data acquisition system was designed to be able to handle sustained data rates of up to 11 520 000 cps without loss (10 000 cps/channel). The timing resolution of the TDC was measured to be 1 ns FWHM. In addition to describing the data acquisition system, performance measurements made using a 128-channel detector prototype will be presented.

  14. Multiplexed Holographic Data Storage in Bacteriorhodopsin

    NASA Technical Reports Server (NTRS)

    Mehrl, David J.; Krile, Thomas F.

    1997-01-01

    High density optical data storage, driven by the information revolution, remains at the forefront of current research areas. Much of the current research has focused on photorefractive materials (SBN and LiNbO3) and polymers, despite various problems with expense, durability, response time and retention periods. Photon echo techniques, though promising, are questionable due to the need for cryogenic conditions. Bacteriorhodopsin (BR) films are an attractive alternative recording medium. Great strides have been made in refining BR, and materials with storage lifetimes as long as 100 days have recently become available. The ability to deposit this robust polycrystalline material as high quality optical films suggests the use of BR as a recording medium for commercial optical disks. Our own recent research has demonstrated the suitability of BR films for real time spatial filtering and holography. We propose to fully investigate the feasibility of performing holographic mass data storage in BR. Important aspects of the problem to be investigated include various data multiplexing techniques (e.g. angle- amplitude- and phase-encoded multiplexing, and in particular shift-multiplexing), multilayer recording techniques, SLM selection and data readout using crossed polarizers for noise rejection. Systems evaluations of storage parameters, including access times, memory refresh constraints, erasure, signal-to-noise ratios and bit error rates, will be included in our investigations.

  15. Orthogonal frequency-division multiplexing access (OFDMA) based wireless visible light communication (VLC) system

    NASA Astrophysics Data System (ADS)

    Sung, Jiun-Yu; Yeh, Chien-Hung; Chow, Chi-Wai; Lin, Wan-Feng; Liu, Yang

    2015-11-01

    An orthogonal frequency-division multiplexing access (OFDMA) based visible light communication (VLC) system is proposed in this paper. The architecture of the proposed system is divided into several VLC cells, which is defined in this paper. The deployment and upgrade of the system involve only simple combination of the VLC cells. Hence it is economically advantageous. To guarantee smooth communication, nearly equal data rate is provided at every location within the system with no concern on the system scale. The user location monitor strategy is also discussed to solve the region division issues. The characteristics of the proposed system are analyzed in detail in this paper. A one-dimensional experiment was demonstrated with 13.6 Mb/s data rate.

  16. Spatio-angularly multiplexed (SAM) holographic storage in photorefractive crystals

    NASA Astrophysics Data System (ADS)

    Tao, Shiquan

    In this thesis a novel multiplexing scheme for dense holographic storage in photorefractive crystals, Spatio-Angular Multiplexing (or SAM), is described in detail. In SAM Fourier transform holograms are formed in spatially overlapping regions of a crystal and are distinguished from one another by using variously angled reference beams. SAM takes advantage of both the high storage density possible using angularly multiplexed volume holograms and also the low crosstalk possible using spatially multiplexed Fourier transform holograms. Compared to pure spatial multiplexing, SAM increases the storage capacity by fully utilising the volume of the storage medium. On the other hand, SAM reduces the number of holograms overlapping any one hologram in a given volume, and so increases the diffraction efficiency achievable as compared to pure angular multiplexing. SAM offers the possibility of incorporating the recorded crystal into a content addressable memory (CAM) system for parallel access of all stored patterns. In order to obtain the maximum diffraction efficiency and signal to noise ratio, the hologram must be replayed by a readout beam incident at the correct angle of readout beam. The optimum angle may be shifted away from the angle used in recording by a ''Bragg-shift", caused (under certain conditions) by phase coupling between the two writing beams during recording. Although this Bragg shift is small, a large diffraction efficiency enhancement is obtained when the grating is read out at the optimum angle. We have calculated the Bragg shift, using a numerical calculation based on an earlier theory, and have obtained good agreement with experiment. Using the novel SAM scheme, we have succeeded in storing 756 high resolution binary patterns in an Fe:LiNbO3 crystal of volume 1cm3, with an average diffraction efficiency of 0.5%. This large database is designed for practical use in a novel associative memory system, called a high order feedback neural network (HOFNET

  17. Development of the 19 X-STR loci multiplex system and genetic analysis of a Zhejiang Han population in China.

    PubMed

    Yang, XingYi; Wu, WeiWei; Chen, LinLi; Liu, ChangHui; Zhang, XiaoFang; Chen, Ling; Feng, XingLin; Wang, HuiJun; Liu, Chao

    2016-08-01

    The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing. PMID:27184937

  18. Characterization of direct readout Si:Sb and Si:Ga infrared detector arrays for space-based astronomy

    NASA Technical Reports Server (NTRS)

    Mckelvey, Mark E.; Mccreight, Craig R.; Goebel, John H.; Moss, Nicolas N.; Savage, Maureen L.

    1988-01-01

    Preliminary test results from the evaluation of Si:Sb and Si:Ga 58 x 62-element infrared detector arrays are presented. These devices are being characterized under background conditions and readout rates representative of operation in orbiting, crogenically-cooled infrared observatories. The arrays are hybridized to silicon direct-readout multiplexers which allow random-access and nondestructive readout. Array performance optimization is being conducted with a flexible microcomputer-based drive and readoaut electronics system. Preliminary Si:Sb measurements indicate a sense node capacitance of 0.06 pF, peak (28-micron) responsivity above 3 A/W at 2V bias, read noise of 130 rms e(-), dark current approximately 10 e(-)/s, and a well capacity greater than 10 to the 5th e(-). The limited test data available on the performance of the Si:Ga array are also discussed.

  19. Sensor readout detector circuit

    DOEpatents

    Chu, D.D.; Thelen, D.C. Jr.

    1998-08-11

    A sensor readout detector circuit is disclosed that is capable of detecting sensor signals down to a few nanoamperes or less in a high (microampere) background noise level. The circuit operates at a very low standby power level and is triggerable by a sensor event signal that is above a predetermined threshold level. A plurality of sensor readout detector circuits can be formed on a substrate as an integrated circuit (IC). These circuits can operate to process data from an array of sensors in parallel, with only data from active sensors being processed for digitization and analysis. This allows the IC to operate at a low power level with a high data throughput for the active sensors. The circuit may be used with many different types of sensors, including photodetectors, capacitance sensors, chemically-sensitive sensors or combinations thereof to provide a capability for recording transient events or for recording data for a predetermined period of time following an event trigger. The sensor readout detector circuit has applications for portable or satellite-based sensor systems. 6 figs.

  20. Sensor readout detector circuit

    DOEpatents

    Chu, Dahlon D.; Thelen, Jr., Donald C.

    1998-01-01

    A sensor readout detector circuit is disclosed that is capable of detecting sensor signals down to a few nanoamperes or less in a high (microampere) background noise level. The circuit operates at a very low standby power level and is triggerable by a sensor event signal that is above a predetermined threshold level. A plurality of sensor readout detector circuits can be formed on a substrate as an integrated circuit (IC). These circuits can operate to process data from an array of sensors in parallel, with only data from active sensors being processed for digitization and analysis. This allows the IC to operate at a low power level with a high data throughput for the active sensors. The circuit may be used with many different types of sensors, including photodetectors, capacitance sensors, chemically-sensitive sensors or combinations thereof to provide a capability for recording transient events or for recording data for a predetermined period of time following an event trigger. The sensor readout detector circuit has applications for portable or satellite-based sensor systems.

  1. A robust and scalable neuromorphic communication system by combining synaptic time multiplexing and MIMO-OFDM.

    PubMed

    Srinivasa, Narayan; Zhang, Deying; Grigorian, Beayna

    2014-03-01

    This paper describes a novel architecture for enabling robust and efficient neuromorphic communication. The architecture combines two concepts: 1) synaptic time multiplexing (STM) that trades space for speed of processing to create an intragroup communication approach that is firing rate independent and offers more flexibility in connectivity than cross-bar architectures and 2) a wired multiple input multiple output (MIMO) communication with orthogonal frequency division multiplexing (OFDM) techniques to enable a robust and efficient intergroup communication for neuromorphic systems. The MIMO-OFDM concept for the proposed architecture was analyzed by simulating large-scale spiking neural network architecture. Analysis shows that the neuromorphic system with MIMO-OFDM exhibits robust and efficient communication while operating in real time with a high bit rate. Through combining STM with MIMO-OFDM techniques, the resulting system offers a flexible and scalable connectivity as well as a power and area efficient solution for the implementation of very large-scale spiking neural architectures in hardware. PMID:24807453

  2. Evaluation of a 13-loci STR multiplex system for Cannabis sativa genetic identification.

    PubMed

    Houston, Rachel; Birck, Matthew; Hughes-Stamm, Sheree; Gangitano, David

    2016-05-01

    Marijuana (Cannabis sativa) is the most commonly used illicit substance in the USA. The development of a validated method using Cannabis short tandem repeats (STRs) could aid in the individualization of samples as well as serve as an intelligence tool to link multiple cases. For this purpose, a modified 13-loci STR multiplex method was optimized and evaluated according to ISFG and SWGDAM guidelines. A real-time PCR quantification method for C. sativa was developed and validated, and a sequenced allelic ladder was also designed to accurately genotype 199 C. sativa samples from 11 U.S. Customs and Border Protection seizures. Distinguishable DNA profiles were generated from 127 samples that yielded full STR profiles. Four duplicate genotypes within seizures were found. The combined power of discrimination of this multilocus system is 1 in 70 million. The sensitivity of the multiplex STR system is 0.25 ng of template DNA. None of the 13 STR markers cross-reacted with any of the studied species, except for Humulus lupulus (hops) which generated unspecific peaks. Phylogenetic analysis and case-to-case pairwise comparison of 11 cases using F st as genetic distance revealed the genetic association of four groups of cases. Moreover, due to their genetic similarity, a subset of samples (N = 97) was found to form a homogeneous population in Hardy-Weinberg and linkage equilibrium. The results of this research demonstrate the applicability of this 13-loci STR system in associating Cannabis cases for intelligence purposes. PMID:26661945

  3. Evaluation of a 13-loci STR multiplex system for Cannabis sativa genetic identification.

    PubMed

    Houston, Rachel; Birck, Matthew; Hughes-Stamm, Sheree; Gangitano, David

    2016-05-01

    Marijuana (Cannabis sativa) is the most commonly used illicit substance in the USA. The development of a validated method using Cannabis short tandem repeats (STRs) could aid in the individualization of samples as well as serve as an intelligence tool to link multiple cases. For this purpose, a modified 13-loci STR multiplex method was optimized and evaluated according to ISFG and SWGDAM guidelines. A real-time PCR quantification method for C. sativa was developed and validated, and a sequenced allelic ladder was also designed to accurately genotype 199 C. sativa samples from 11 U.S. Customs and Border Protection seizures. Distinguishable DNA profiles were generated from 127 samples that yielded full STR profiles. Four duplicate genotypes within seizures were found. The combined power of discrimination of this multilocus system is 1 in 70 million. The sensitivity of the multiplex STR system is 0.25 ng of template DNA. None of the 13 STR markers cross-reacted with any of the studied species, except for Humulus lupulus (hops) which generated unspecific peaks. Phylogenetic analysis and case-to-case pairwise comparison of 11 cases using F st as genetic distance revealed the genetic association of four groups of cases. Moreover, due to their genetic similarity, a subset of samples (N = 97) was found to form a homogeneous population in Hardy-Weinberg and linkage equilibrium. The results of this research demonstrate the applicability of this 13-loci STR system in associating Cannabis cases for intelligence purposes.

  4. An Integrated Front-End Readout And Feature Extraction System for the BaBar Drift Chamber

    SciTech Connect

    Zhang, Jinlong; /Colorado U.

    2006-08-10

    The BABAR experiment has been operating at SLAC's PEP-II asymmetric B-Factory since 1999. The accelerator has achieved more than three times its original design luminosity of 3 x 10{sup 33} cm{sup -2} s{sup -1}, with plans for an additional factor of three in the next two years. To meet the experiment's performance requirements in the face of significantly higher trigger and background rates, the drift chamber's front-end readout system has been redesigned around the Xilinx Spartan 3 FPGA. The new system implements analysis and feature-extraction of digitized waveforms in the front-end, reducing the data bandwidth required by a factor of four.

  5. The impact of multiplexing on the dynamic requirements of analog-to-digital converters

    SciTech Connect

    Sobering, T.J.; Kay, R.R.

    1994-12-31

    In data acquisition applications where the signals being digitized are produced in a time-division multiplexed system, the required dynamic performance of the analog-to-digital converter (ADC) is no longer bound by the conditions set forth in the Sampling Theorem. This results from the introduction of very high frequency information by the multiplexing process which, while not necessarily containing information of interest, must be processed by the input circuitry of the ADC. In this situation, signal bandwidths and slew rates can greatly exceed those produced in a Nyquist limited system and can surpass the capability of the ADC, thus degrading overall system performance. This paper will examine two common multiplexing schemes and their impact on ADC dynamic requirements. First, we will examine a simple voltage multiplexing scheme typically found in state-ofhealth or data-logging applications and develop the necessary equations to show how the ADC dynamic requirements are affected. Then, the analysis will then be extended to a multiplexed photodiode array readout to see how this application further challenges the dynamic performance of the ADC. Finally, the issues associated with developing dynamic test methodologies for assessing ADC performance in multiplexed systems will be discussed.

  6. A low-noise 64-channel front-end readout ASIC for CdZnTe detectors aimed to hard X-ray imaging systems

    NASA Astrophysics Data System (ADS)

    Gan, B.; Wei, T.; Gao, W.; Liu, H.; Hu, Y.

    2016-04-01

    In this paper, we report on the recent development of a 64-channel low-noise front-end readout ASIC for CdZnTe detectors aimed to hard X-ray imaging systems. The readout channel is comprised of a charge sensitive amplifier, a leakage current compensation circuit, a CR-RC shaper, two S-K filters, an inverse proportional amplifier, a peak-detect-and-hold circuit, a discriminator and trigger logic, a time sequence control circuit and a driving buffer. The readout ASIC is implemented in TSMC 0.35 μm mixed-signal CMOS technology, the die size of the prototype chip is 2.7 mm×8.0 mm. The overall gain of the readout channel is 200 mV/fC, the power consumption is less than 8 mW/channel, the linearity error is less than 1%, the inconsistency among the channels is less than 2.86%, and the equivalent noise charge of a typical channel is 66 e- at zero farad plus 14 e- per picofarad. By connecting this readout ASIC to an 8×8 pixel CdZnTe detector, we obtained an energy spectrum, the energy resolution of which is 4.5% at the 59.5 keV line of 241Am source.

  7. Low complexity digital backpropagation for high baud subcarrier-multiplexing systems.

    PubMed

    Zhang, Fangyuan; Zhuge, Qunbi; Qiu, Meng; Plant, David V

    2016-07-25

    In this paper, we propose two modifications to reduce the complexity of the subcarrier-multiplexing (SCM) based digital backpropagation (DBP) for high symbol rate SCM systems. The first one is to reduce the number of interfering subcarriers (RS-SCM-DBP) when evaluating the cross-subcarrier nonlinearity (CSN). The second one is to replace the original frequency domain CSN filters with the infinite impulse response (IIR) filters (IIR-RS-SCM-DBP) in the CSN compensation. The performance of the proposed schemes are numerically evaluated in three-channel dual-polarization (DP) 16QAM wavelength-division multiplexing (WDM) transmissions. The aggregate symbol rate for each channel is 120 GBaud and the transmission distance is 1600 km. For the SCM system with 16 subcarriers, the IIR-RS-SCM-DBP with only 4 interfering subcarriers and 2 steps can achieve a 0.3 dB Q-factor improvement in the WDM transmission. Compared to the original SCM-DBP, the proposed IIR-RS-SCM-DBP reduces the complexity by 48% at a performance loss of only 0.07 dB.

  8. Compressed sensing theory-based channel estimation for optical orthogonal frequency division multiplexing communication system

    NASA Astrophysics Data System (ADS)

    Zhao, Hui; Li, Minghui; Wang, Ruyan; Liu, Yuanni; Song, Daiping

    2014-09-01

    Due to the spare multipath property of the channel, a channel estimation method, which is based on partial superimposed training sequence and compressed sensing theory, is proposed for line of sight optical orthogonal frequency division multiplexing communication systems. First, a continuous training sequence is added at variable power ratio to the cyclic prefix of orthogonal frequency division multiplexing symbols at the transmitter prior to transmission. Then the observation matrix of compressed sensing theory is structured by the use of the training symbols at receiver. Finally, channel state information is estimated using sparse signal reconstruction algorithm. Compared to traditional training sequences, the proposed partial superimposed training sequence not only improves the spectral efficiency, but also reduces the influence to information symbols. In addition, compared with classical least squares and linear minimum mean square error methods, the proposed compressed sensing theory based channel estimation method can improve both the estimation accuracy and the system performance. Simulation results are given to demonstrate the performance of the proposed method.

  9. Electra: Repetitively Pulsed Angularly Multiplexed KrF Laser System Performance

    NASA Astrophysics Data System (ADS)

    Wolford, Matthew; Myers, Matthew; Giuliani, John; Sethian, John; Burns, Patrick; Hegeler, Frank; Jaynes, Reginald

    2008-11-01

    As in a full size fusion power plant beam line, Electra is a multistage laser amplifier system. The multistage amplifier system consists of a commercial discharge laser and two doubled sided electron beam pumped amplifiers. Angular multiplexing is used in the optical layout to provide pulse length control and to maximize laser extraction from the amplifiers. Two angularly multiplexed beams have extracted 30 J of KrF laser light with an aperture 8 x 10 cm^2, which is sufficient to extract over 500 J from the main amplifier and models agree. The main amplifier of Electra in oscillator mode has demonstrated single shot and rep-rate laser energies exceeding 700 J with 100 ns pulsewidth at 248 nm with an aperture 29 x 29 cm^2. Continuous operation of the KrF electron beam pumped oscillator has lasted for more than 2.5 hours without failure at 1 Hz and 2.5 Hz. The measured intensity and pulse energy for durations greater than thousand shots are consistent at measurable rep-rates of 1 Hz, 2.5 Hz and 5 Hz.

  10. A compact time-resolved system for near infrared spectroscopy based on wavelength space multiplexing

    NASA Astrophysics Data System (ADS)

    Re, Rebecca; Contini, Davide; Caffini, Matteo; Cubeddu, Rinaldo; Spinelli, Lorenzo; Torricelli, Alessandro

    2010-11-01

    We designed and developed a compact dual-wavelength and dual-channel time-resolved system for near-infrared spectroscopy studies of muscle and brain. The system employs pulsed diode lasers as sources, compact photomultipliers, and time-correlated single photon counting boards for detection. To exploit the full temporal and dynamic range of the acquisition technique, we implemented an approach based on wavelength space multiplexing: laser pulses at the two wavelengths are alternatively injected into the two channels by means of an optical 2×2 switch. In each detection line (i.e., in each temporal window), the distribution of photon time-of-flights at one wavelength is acquired. The proposed approach increases the signal-to-noise ratio and avoids wavelength cross-talk with respect to the typical approach based on time multiplexing. The instrument was characterized on tissue phantoms to assess its properties in terms of linearity, stability, noise, and reproducibility. Finally, it was successfully tested in preliminary in vivo measurements on muscle during standard cuff occlusion and on the brain during a motor cortex response due to hand movements.

  11. A multiplexing fiber optic microsensor system for monitoring oxygen concentration in plants

    NASA Astrophysics Data System (ADS)

    Chaturvedi, P.; Hauser, B. A.; Allen, L. H.; Boote, K. J.; Karplus, E.; McLamore, E. S.

    2013-05-01

    The accurate and rapid measurement of physiological O2 transport is vital for understanding spatially and temporally dynamic metabolism and stress signalling in plant cells and tissues. Single channel luminescent O2- quenched optrodes have been available for use in laboratory and field experiments since the early 2000's. However, to collect the large datasets needed to understand O2 transport in complex systems, many experiments require a multiple channel O2 sensor system. This research reports the development of a multiplexing fiber optic O2 microsensor system designed to conduct high temporal resolution experiments for field studies of plant physiology. The 10 channel system was demonstrated for measuring O2 concentration in developing soybean seeds (Glycine max L. Merr.) within a climate controlled greenhouse.

  12. Multiplex transmission system for gate drive signals of inverter circuit using surface acoustic wave filters

    NASA Astrophysics Data System (ADS)

    Suzuki, Akifumi; Ueda, Kensuke; Goka, Shigeyoshi; Wada, Keiji; Kakio, Shoji

    2016-07-01

    We propose and fabricate a multiplexed transmission system based on frequency-division multiple access (FDMA) with surface acoustic wave (SAW) filters. SAW filters are suitable for use in wide-gap switching devices and multilevel inverters because of their capability to operate at high temperatures, good electrical isolation, low cost, and high reliability. Our proposed system reduces the number of electrical signal wires needed to control each switching device and eliminates the need for isolation circuits, simplifying the transmission system and gate drive circuits. We successfully controlled two switching devices with a single coaxial line and confirmed the operation of a single-phase half-bridge inverter at a supply voltage of 100 V, and the total delay time to control the switching devices was less than 2.5 µs. Our experimental results validated our proposed system.

  13. Weighted multiplex networks.

    PubMed

    Menichetti, Giulia; Remondini, Daniel; Panzarasa, Pietro; Mondragón, Raúl J; Bianconi, Ginestra

    2014-01-01

    One of the most important challenges in network science is to quantify the information encoded in complex network structures. Disentangling randomness from organizational principles is even more demanding when networks have a multiplex nature. Multiplex networks are multilayer systems of [Formula: see text] nodes that can be linked in multiple interacting and co-evolving layers. In these networks, relevant information might not be captured if the single layers were analyzed separately. Here we demonstrate that such partial analysis of layers fails to capture significant correlations between weights and topology of complex multiplex networks. To this end, we study two weighted multiplex co-authorship and citation networks involving the authors included in the American Physical Society. We show that in these networks weights are strongly correlated with multiplex structure, and provide empirical evidence in favor of the advantage of studying weighted measures of multiplex networks, such as multistrength and the inverse multiparticipation ratio. Finally, we introduce a theoretical framework based on the entropy of multiplex ensembles to quantify the information stored in multiplex networks that would remain undetected if the single layers were analyzed in isolation.

  14. Multiplexed infrared photodetection using resonant radio-frequency circuits

    NASA Astrophysics Data System (ADS)

    Liu, R.; Lu, R.; Roberts, C.; Gong, S.; Allen, J. W.; Allen, M. S.; Wenner, B. R.; Wasserman, D.

    2016-02-01

    We demonstrate a room-temperature semiconductor-based photodetector where readout is achieved using a resonant radio-frequency (RF) circuit consisting of a microstrip split-ring resonator coupled to a microstrip busline, fabricated on a semiconductor substrate. The RF resonant circuits are characterized at RF frequencies as function of resonator geometry, as well as for their response to incident IR radiation. The detectors are modeled analytically and using commercial simulation software, with good agreement to our experimental results. Though the detector sensitivity is weak, the detector architecture offers the potential for multiplexing arrays of detectors on a single read-out line, in addition to high speed response for either direct coupling of optical signals to RF circuitry, or alternatively, carrier dynamics characterization of semiconductor, or other, material systems.

  15. Frequency domain multiplexing for large-scale bolometer arrays

    SciTech Connect

    Spieler, Helmuth

    2002-05-31

    The development of planar fabrication techniques for superconducting transition-edge sensors has brought large-scale arrays of 1000 pixels or more to the realm of practicality. This raises the problem of reading out a large number of sensors with a tractable number of connections. A possible solution is frequency-domain multiplexing. I summarize basic principles, present various circuit topologies, and discuss design trade-offs, noise performance, cross-talk and dynamic range. The design of a practical device and its readout system is described with a discussion of fabrication issues, practical limits and future prospects.

  16. A non-destructive readout circuit of the linear array image sensor with over 90dB dynamic range and 190k fps for radar system

    NASA Astrophysics Data System (ADS)

    Yang, Cong-jie; Gao, Zhi-yuan; Zeng, Xin-ji; Yao, Su-ying; Gao, Jing

    2015-04-01

    This paper presents a non-destructive readout circuit of the linear array image sensor with wide dynamic range and high speed readout for radar system. A multi-capacitor and self-regulated capacitive trans-impedance amplifier (CTIA) structure is employed to extend the dynamic range. The gain of the CTIA is auto adjusted by switching different capacitors to the integration node asynchronously according to the output voltage. A class AB OPA is utilized to drive all the additional capacitors to achieve high speed readout. A photo response curve presents as a polyline with 5 segments, which enables a 101.7 dB dynamic range. In addition, the exposure time is 5.12us in the simulation, then an over 190k fps is achieved.

  17. Experimental investigation of silicon photomultipliers as compact light readout systems for gamma-ray spectroscopy applications in fusion plasmas.

    PubMed

    Nocente, M; Fazzi, A; Tardocchi, M; Cazzaniga, C; Lorenzoli, M; Pirovano, C; Rebai, M; Uboldi, C; Varoli, V; Gorini, G

    2014-11-01

    A matrix of Silicon Photo Multipliers has been developed for light readout from a large area 1 in. × 1 in. LaBr3 crystal. The system has been characterized in the laboratory and its performance compared to that of a conventional photo multiplier tube. A pulse duration of 100 ns was achieved, which opens up to spectroscopy applications at high counting rates. The energy resolution measured using radioactive sources extrapolates to 3%-4% in the energy range Eγ = 3-5 MeV, enabling gamma-ray spectroscopy measurements at good energy resolution. The results reported here are of relevance in view of the development of compact gamma-ray detectors with spectroscopy capabilities, such as an enhanced gamma-ray camera for high power fusion plasmas, where the use of photomultiplier is impeded by space limitation and sensitivity to magnetic fields. PMID:25430287

  18. Experimental investigation of silicon photomultipliers as compact light readout systems for gamma-ray spectroscopy applications in fusion plasmas

    SciTech Connect

    Nocente, M. Gorini, G.; Fazzi, A.; Lorenzoli, M.; Pirovano, C.; Tardocchi, M.; Cazzaniga, C.; Rebai, M.; Uboldi, C.; Varoli, V.

    2014-11-15

    A matrix of Silicon Photo Multipliers has been developed for light readout from a large area 1 in. × 1 in. LaBr{sub 3} crystal. The system has been characterized in the laboratory and its performance compared to that of a conventional photo multiplier tube. A pulse duration of 100 ns was achieved, which opens up to spectroscopy applications at high counting rates. The energy resolution measured using radioactive sources extrapolates to 3%–4% in the energy range E{sub γ} = 3–5 MeV, enabling gamma-ray spectroscopy measurements at good energy resolution. The results reported here are of relevance in view of the development of compact gamma-ray detectors with spectroscopy capabilities, such as an enhanced gamma-ray camera for high power fusion plasmas, where the use of photomultiplier is impeded by space limitation and sensitivity to magnetic fields.

  19. Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system.

    PubMed

    Yin, L; Maddison, L A; Chen, W

    2016-01-01

    The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a powerful tool for genome editing in numerous organisms. However, the system is typically used for gene editing throughout the entire organism. Tissue and temporal specific mutagenesis is often desirable to determine gene function in a specific stage or tissue and to bypass undesired consequences of global mutations. We have developed the CRISPR/Cas system for conditional mutagenesis in transgenic zebrafish using tissue-specific and/or inducible expression of Cas9 and U6-driven expression of sgRNA. To allow mutagenesis of multiple targets, we have isolated four distinct U6 promoters and designed Golden Gate vectors to easily assemble transgenes with multiple sgRNAs. We provide experimental details on the reagents and applications for multiplex conditional mutagenesis in zebrafish. PMID:27443918

  20. Automatic readout micrometer

    DOEpatents

    Lauritzen, T.

    A measuring system is described for surveying and very accurately positioning objects with respect to a reference line. A principle use of this surveying system is for accurately aligning the electromagnets which direct a particle beam emitted from a particle accelerator. Prior art surveying systems require highly skilled surveyors. Prior art systems include, for example, optical surveying systems which are susceptible to operator reading errors, and celestial navigation-type surveying systems, with their inherent complexities. The present invention provides an automatic readout micrometer which can very accurately measure distances. The invention has a simplicity of operation which practically eliminates the possibilities of operator optical reading error, owning to the elimination of traditional optical alignments for making measurements. The invention has an extendable arm which carries a laser surveying target. The extendable arm can be continuously positioned over its entire length of travel by either a coarse of fine adjustment without having the fine adjustment outrun the coarse adjustment until a reference laser beam is centered on the target as indicated by a digital readout. The length of the micrometer can then be accurately and automatically read by a computer and compared with a standardized set of alignment measurements. Due to its construction, the micrometer eliminates any errors due to temperature changes when the system is operated within a standard operating temperature range.

  1. Automatic readout micrometer

    DOEpatents

    Lauritzen, Ted

    1982-01-01

    A measuring system is disclosed for surveying and very accurately positioning objects with respect to a reference line. A principal use of this surveying system is for accurately aligning the electromagnets which direct a particle beam emitted from a particle accelerator. Prior art surveying systems require highly skilled surveyors. Prior art systems include, for example, optical surveying systems which are susceptible to operator reading errors, and celestial navigation-type surveying systems, with their inherent complexities. The present invention provides an automatic readout micrometer which can very accurately measure distances. The invention has a simplicity of operation which practically eliminates the possibilities of operator optical reading error, owning to the elimination of traditional optical alignments for making measurements. The invention has an extendable arm which carries a laser surveying target. The extendable arm can be continuously positioned over its entire length of travel by either a coarse or fine adjustment without having the fine adjustment outrun the coarse adjustment until a reference laser beam is centered on the target as indicated by a digital readout. The length of the micrometer can then be accurately and automatically read by a computer and compared with a standardized set of alignment measurements. Due to its construction, the micrometer eliminates any errors due to temperature changes when the system is operated within a standard operating temperature range.

  2. Digital subcarrier multiplexing for fiber nonlinearity mitigation in coherent optical communication systems.

    PubMed

    Qiu, Meng; Zhuge, Qunbi; Chagnon, Mathieu; Gao, Yuliang; Xu, Xian; Morsy-Osman, Mohamed; Plant, David V

    2014-07-28

    In this work we experimentally investigate the improved intra-channel fiber nonlinearity tolerance of digital subcarrier multiplexed (SCM) signals in a single-channel coherent optical transmission system. The digital signal processing (DSP) for the generation and reception of the SCM signals is described. We show experimentally that the SCM signal with a nearly-optimum number of subcarriers can extend the maximum reach by 23% in a 24 GBaud DP-QPSK transmission with a BER threshold of 3.8 × 10(-3) and by 8% in a 24 GBaud DP-16-QAM transmission with a BER threshold of 2 × 10(-2). Moreover, we show by simulations that the improved performance of SCM signals is observed over a wide range of baud rates, further indicating the merits of SCM signals in baud-rate flexible agile transmissions and future high-speed optical transport systems.

  3. A multiplexed transcription activator-like effector system for detecting specific DNA sequences.

    PubMed

    Honarmand, Ali; Mayall, Robert; George, Iain; Oberding, Lisa; Dastidar, Himika; Fegan, Jamie; Chaudhuri, Somshukla; Dole, Justin; Feng, Sharon; Hoang, Denny; Moges, Ruth; Osgood, Julie; Remondini, Taylor; van der Meulen, Wm Keith; Wang, Su; Wintersinger, Chris; Zaparoli Zucoloto, Amanda; Chatfield-Reed, Kate; Arcellana-Panlilio, Mayi; Nygren, Anders

    2014-12-19

    Transcription activator-like effectors (TALEs), originating from the Xanthomonas genus of bacteria, bind to specific DNA sequences based on amino acid sequence in the repeat-variable diresidue (RVD) positions of the protein. By altering these RVDs, it has been shown that a TALE protein can be engineered to bind virtually any DNA sequence of interest. The possibility of multiplexing TALEs for the purposes of identifying specific DNA sequences has yet to be explored. Here, we demonstrate a system in which a TALE protein bound to a nitrocellulose strip has been utilized to capture purified DNA, which is then detected using the binding of a second distinct TALE protein conjugated to a protein tag that is then detected by a dot blot. This system provides a signal only when both TALEs bind to their respective sequences, further demonstrating the specificity of the TALE binding.

  4. A multiplexed transcription activator-like effector system for detecting specific DNA sequences.

    PubMed

    Honarmand, Ali; Mayall, Robert; George, Iain; Oberding, Lisa; Dastidar, Himika; Fegan, Jamie; Chaudhuri, Somshukla; Dole, Justin; Feng, Sharon; Hoang, Denny; Moges, Ruth; Osgood, Julie; Remondini, Taylor; van der Meulen, Wm Keith; Wang, Su; Wintersinger, Chris; Zaparoli Zucoloto, Amanda; Chatfield-Reed, Kate; Arcellana-Panlilio, Mayi; Nygren, Anders

    2014-12-19

    Transcription activator-like effectors (TALEs), originating from the Xanthomonas genus of bacteria, bind to specific DNA sequences based on amino acid sequence in the repeat-variable diresidue (RVD) positions of the protein. By altering these RVDs, it has been shown that a TALE protein can be engineered to bind virtually any DNA sequence of interest. The possibility of multiplexing TALEs for the purposes of identifying specific DNA sequences has yet to be explored. Here, we demonstrate a system in which a TALE protein bound to a nitrocellulose strip has been utilized to capture purified DNA, which is then detected using the binding of a second distinct TALE protein conjugated to a protein tag that is then detected by a dot blot. This system provides a signal only when both TALEs bind to their respective sequences, further demonstrating the specificity of the TALE binding. PMID:25524096

  5. A data readout approach for physics experiments

    NASA Astrophysics Data System (ADS)

    Huang, Xi-Ru; Cao, Ping; Gao, Li-Wei; Zheng, Jia-Jun

    2015-07-01

    With increasing physical event rates and the number of electronic channels, traditional readout schemes meet the challenge of improving readout speed caused by the limited bandwidth of the crate backplane. In this paper, a high-speed data readout method based on the Ethernet is presented to make each readout module capable of transmitting data to the DAQ. Features of explicitly parallel data transmitting and distributed network architecture give the readout system the advantage of adapting varying requirements of particle physics experiments. Furthermore, to guarantee the readout performance and flexibility, a standalone embedded CPU system is utilized for network protocol stack processing. To receive the customized data format and protocol from front-end electronics, a field programmable gate array (FPGA) is used for logic reconfiguration. To optimize the interface and to improve the data throughput between CPU and FPGA, a sophisticated method based on SRAM is presented in this paper. For the purpose of evaluating this high-speed readout method, a simplified readout module is designed and implemented. Test results show that this module can support up to 70 Mbps data throughput from the readout module to DAQ. Supported by National Natural Science Foundation of China (11005107) and Independent Projects of State Key Laboratory of Particle Detection and Electronics (201301)

  6. Multiplexed image storage by electromagnetically induced transparency in a solid

    NASA Astrophysics Data System (ADS)

    Heinze, G.; Rentzsch, N.; Halfmann, T.

    2012-11-01

    We report on frequency- and angle-multiplexed image storage by electromagnetically induced transparency (EIT) in a Pr3+:Y2SiO5 crystal. Frequency multiplexing by EIT relies on simultaneous storage of light pulses in atomic coherences, driven in different frequency ensembles of the inhomogeneously broadened solid medium. Angular multiplexing by EIT relies on phase matching of the driving laser beams, which permits simultaneous storage of light pulses propagating under different angles into the crystal. We apply the multiplexing techniques to increase the storage capacity of the EIT-driven optical memory, in particular to implement multiplexed storage of larger two-dimensional amounts of data (images). We demonstrate selective storage and readout of images by frequency-multiplexed EIT and angular-multiplexed EIT, as well as the potential to combine both multiplexing approaches towards further enhanced storage capacities.

  7. Channel estimation for asymmetrically clipped optical orthogonal frequency division multiplexing communication system

    NASA Astrophysics Data System (ADS)

    Zhao, Hui; Li, Minghui; Wang, Ruyan; Wu, Dapeng

    2013-07-01

    The channel estimation problem for asymmetrically clipped optical orthogonal frequency division multiplexing wireless communication systems is investigated. In order to resolve the noise-sensitive problem of traditional least squares-based channel estimation method, a new channel estimation method which is based on superimposed training sequence and guarantees the linear minimum mean square error estimate is proposed. Cycle training sequence is added at variable power ratio to the information sequence at the transmitter prior to transmission. Then, statistical average method is employed to separate training and information sequences at the receiver. Simulation results show that the power ratio of training sequence needs to balance between the mean square error (MSE) of estimation and the error bit rate. Moreover, compared with the traditional least squares-based method, the proposed method has significantly improved the estimation performance under the condition of low signal-to-noise ratio, especially, when the MSE of the estimation reduces 1 to 2 orders.

  8. Design of the multiplexing communication system with non-coherent vortex beams

    NASA Astrophysics Data System (ADS)

    Zhao, Hongdong; Peng, Xiaocan; Ma, Li; Sun, Mei

    2016-11-01

    In order to enlarge the communication capability, a model of the multiplexing communication system with non-coherent vortex beams is established. One detector for measurement the signal of the vortex beam with topological charge of 0, which is a Gaussian beam, is located in the center of the cross sectional plane of vortex beam. The other three detectors are set around the first detector in the same plane to receive the power of the vortex beam with topological charge of 1. The principle of determining the emitting power of vortex beams, the radii and the positions of the detectors are suggested to increase the signals and reduce the interchannel crosstalk noise at the detectors. The signal powers as well as the interchannel crosstalk noise in a receiver channel are identical to that in another channel, respectively. This research may have applications in free space optical communications.

  9. SQUID Multiplexers for Cryogenic Detector Arrays

    NASA Technical Reports Server (NTRS)

    Irwin, Kent; Beall, James; Deiker, Steve; Doriese, Randy; Duncan, William; Hilton, Gene; Moseley, S. Harvey; Reintsema, Carl; Stahle, Caroline; Ullom, Joel; Vale, Leila

    2004-01-01

    SQUID multiplexers make it possible to build arrays of thousands of cryogenic detectors with a manageable number of readout channels. We are developing time-division SQUID multiplexers based on Nb trilayer SQUIDs to read arrays of superconducting transition-edge sensors. Our first-generation, 8-channel SQUID multiplexer was used in FIBRE, a one-dimensional TES array for submillimeter astronomy. Our second-generation 32-pixel multiplexer, based on an improved architecture, has been developed for instruments including Constellation-X, SCUBA-2, and solar x-ray astronomy missions. SCUBA-2, which is being developed for the James Clerk Maxwell Telescope, will have more than 10,000 pixels. We are now developing a third-generation architecture based on superconducting hot-electron switches. The use of SQUID multiplexers in instruments operating at above 2 K will also be discussed.

  10. Experimental investigation of an optically amplified time-division-multiplexed polarization-insensitive fiber-optic michelson interferometric sensor system.

    PubMed

    Chen, Y K; Law, P C; Huang, S C

    1998-10-01

    We experimentally investigate optically amplified time-division-multiplexed polarization-insensitive fiber-optic Michelson interferometric (PIFOMI) sensor systems, using an erbium-doped fiber amplifier (EDFA) and a phase-generated carrier (PGC) demodulation technique. The influence of the EDFA on the extinction ratio (ER) of the light pulse and on the minimum phase-detection sensitivity (MPDS) is examined. We find that the EDFA acting as a preamplifier has limited usefulness because the highly amplified spontaneous emission (ASE) noise generated by the EDFA degrades the ER and the MPDS. However, both postamplifiers and in-line EDFA's can work successfully. The MPDS of the unamplified time-division-multiplexed PIFOMI system with an ER of 33 dB was 2.4 x 10(-5) rad/(Hz)(1/2) at ~1 kHz. For maintaining a MPDS of better than 3.4 x 10(-5) rad/(Hz)(1/2) at ~1 kHz, the worst ER's for the postamplified and in-line amplified systems were 20 and 17.8 dB, respectively. The corresponding input signal peak power should be larger than -20 and -25 dBm for the postamplifiers and in-line amplifiers, respectively. When two postamplifiers and two in-line amplifiers are used, an allowable sensor system loss of 47 dB and a link length of the input-output lead fiber of 108 km can be realized for this system with a 32-sensor array. Implementation of optically amplified time-division-multiplexed and wavelength-division multiplexed-time-division multiplexed PIFOMI subarray sensor systems are also addressed.

  11. Multiplexing in optical encryption by using an aperture system and a rotating sandwich random phase diffuser in the Fourier plane

    NASA Astrophysics Data System (ADS)

    Singh, Madan; Kumar, Arvind; Singh, Kehar

    2008-03-01

    In the present paper, we describe multiplexing in optical encryption of two-dimensional images, by using apertures and rotation of one of the constituent phase diffusers of a sandwich phase diffuser in the Fourier plane. The sandwich phase diffuser is made with two random constituent phase-diffusing surfaces sandwiched together. The apertures of different sizes and shapes are made with the help of 'paint brush' software. Simulation results are presented showing the effects of size, shape, and orientation of the apertures on the decrypted images obtained via multiplexing techniques. In addition to the results of using aperture systems in encryption and decryption, the results of rotation of one of the constituent phase diffusers in decryption are analyzed and used in multiplexing. Due to the use of aperture systems and the rotation of the constituent phase diffuser, the multiplexing capability of the system in encryption is enhanced along with the enhanced security due to using a sandwich diffuser. To evaluate the reliability of the technique, mean square error between the decrypted and the original image has been calculated.

  12. Millisecond readout CCD camera

    NASA Astrophysics Data System (ADS)

    Prokop, Mark; McCurnin, Thomas W.; Stradling, Gary L.

    1993-01-01

    We have developed a prototype of a fast-scanning CCD readout system to record a 1024 X 256 pixel image and transport the image to a recording station within 1 ms of the experimental event. The system is designed to have a dynamic range of greater than 1000 with adequate sensitivity to read single-electron excitations of a CRT phosphor when amplified by a microchannel plate image intensifier. This readout camera is intended for recording images from oscilloscopes, streak, and framing cameras. The sensor is a custom CCD chip, designed by LORAL Aeroneutronics. This CCD chip is designed with 16 parallel output ports to supply the necessary image transfer speed. The CCD is designed as an interline structure to allow fast clearing of the image and on-chip fast sputtering. Special antiblooming provisions are also included. The camera is designed to be modular and to allow CCD chips of other sizes to be used with minimal reengineering of the camera head.

  13. Millisecond readout CCD camera

    NASA Astrophysics Data System (ADS)

    Prokop, M.; McCurnin, T. W.; Stradling, G.

    We have developed a prototype of a fast-scanning CCD readout system to record a 1024 x 256 pixel image and transport the image to a recording station within 1 ms of the experimental event. The system is designed to have a dynamic range of greater than 1000 with adequate sensitivity to read single-electron excitations of a CRT phosphor when amplified by a microchannel plate image intensifier. This readout camera is intended for recording images from oscilloscopes, streak, and framing cameras. The sensor is a custom CCD chip, designed by LORAL Aeroneutronics. This CCD chip is designed with 16 parallel output ports to supply the necessary image transfer speed. The CCD is designed as an interline structure to allow fast clearing of the image and on-chip fast shuttering. Special antiblooming provisions are also included. The camera is designed to be modular and to allow CCD chips of other sizes to be used with minimal reengineering of the camera head.

  14. Group-Specific Multiplex PCR Detection Systems for the Identification of Flying Insect Prey

    PubMed Central

    Sint, Daniela; Niederklapfer, Bettina; Kaufmann, Ruediger; Traugott, Michael

    2014-01-01

    The applicability of species-specific primers to study feeding interactions is restricted to those ecosystems where the targeted prey species occur. Therefore, group-specific primer pairs, targeting higher taxonomic levels, are often desired to investigate interactions in a range of habitats that do not share the same species but the same groups of prey. Such primers are also valuable to study the diet of generalist predators when next generation sequencing approaches cannot be applied beneficially. Moreover, due to the large range of prey consumed by generalists, it is impossible to investigate the breadth of their diet with species-specific primers, even if multiplexing them. However, only few group-specific primers are available to date and important groups of prey such as flying insects have rarely been targeted. Our aim was to fill this gap and develop group-specific primers suitable to detect and identify the DNA of common taxa of flying insects. The primers were combined in two multiplex PCR systems, which allow a time- and cost-effective screening of samples for DNA of the dipteran subsection Calyptratae (including Anthomyiidae, Calliphoridae, Muscidae), other common dipteran families (Phoridae, Syrphidae, Bibionidae, Chironomidae, Sciaridae, Tipulidae), three orders of flying insects (Hymenoptera, Lepidoptera, Plecoptera) and coniferous aphids within the genus Cinara. The two PCR assays were highly specific and sensitive and their suitability to detect prey was confirmed by testing field-collected dietary samples from arthropods and vertebrates. The PCR assays presented here allow targeting prey at higher taxonomic levels such as family or order and therefore improve our ability to assess (trophic) interactions with flying insects in terrestrial and aquatic habitats. PMID:25525799

  15. RF Single Electron Transistor Readout Amplifiers for Superconducting Astronomical Detectors for X-Ray to Sub-mm Wavelengths

    NASA Technical Reports Server (NTRS)

    Stevenson, Thomas; Aassime, Abdelhanin; Delsing, Per; Frunzio, Luigi; Li, Li-Qun; Prober, Daniel; Schoelkopf, Robert; Segall, Ken; Wilson, Chris; Stahle, Carl

    2000-01-01

    We report progress on using a new type of amplifier, the Radio-Frequency Single-Electron Transistor (RF-SET), to develop multi-channel sensor readout systems for fast and sensitive readout of high impedance cryogenic photodetectors such as Superconducting Tunnel Junctions and Single Quasiparticle Photon Counters. Although cryogenic, these detectors are desirable because of capabilities not other-wise attainable. However, high impedances and low output levels make low-noise, high-speed readouts challenging, and large format arrays would be facilitated by compact, low-power, on-chip integrated amplifiers. Well-suited for this application are RF-SETs, very high performance electrometers which use an rf readout technique to provide 100 MHz bandwidth. Small size, low power, and cryogenic operation allow direct integration with detectors, and using multiple rf carrier frequencies permits simultaneous readout of 20-50 amplifiers with a common electrical connection. We describe both the first 2-channel demonstration of this wavelength division multiplexing technique for RF-SETs, and Charge-Locked-Loop operation with 100 kHz of closed-loop bandwidth.

  16. A high-performance miniaturized time division multiplexed sensor system for remote structural health monitoring

    NASA Astrophysics Data System (ADS)

    Lloyd, Glynn D.; Everall, Lorna A.; Sugden, Kate; Bennion, Ian

    2004-09-01

    We report for the first time the design, implementation and commercial application of a hand-held optical time division multiplexed, distributed fibre Bragg grating sensor system. A unique combination of state-of-the art electronic and optical components enables system miniaturization whilst maintaining exceptional performance. Supporting more than 100 low-cost sensors per channel, the battery-powered system operates remotely via a wireless GSM link, making it ideal for real-time structural health monitoring in harsh environments. Driven by highly configurable timing electronics, an off-the-shelf telecommunications semiconductor optical amplifier performs combined amplification and gating. This novel optical configuration boasts a spatial resolution of less than 20cm and an optical signal to noise ratio of better than 30dB, yet utilizes sensors with reflectivity of only a few percent and does not require RF speed signal processing devices. This paper highlights the performance and cost advantages of a system that utilizes TDM-style mass manufactured commodity FBGs. Created in continual lengths, these sensors reduce stock inventory, eradicate application-specific array design and simplify system installation and expansion. System analysis from commercial installations in oil exploration, wind energy and vibration measurement will be presented, with results showing kilohertz interrogation speed and microstrain resolution.

  17. Linear readout of object manifolds

    NASA Astrophysics Data System (ADS)

    Chung, SueYeon; Lee, Daniel D.; Sompolinsky, Haim

    2016-06-01

    Objects are represented in sensory systems by continuous manifolds due to sensitivity of neuronal responses to changes in physical features such as location, orientation, and intensity. What makes certain sensory representations better suited for invariant decoding of objects by downstream networks? We present a theory that characterizes the ability of a linear readout network, the perceptron, to classify objects from variable neural responses. We show how the readout perceptron capacity depends on the dimensionality, size, and shape of the object manifolds in its input neural representation.

  18. A readout for large arrays of microwave kinetic inductance detectors.

    PubMed

    McHugh, Sean; Mazin, Benjamin A; Serfass, Bruno; Meeker, Seth; O'Brien, Kieran; Duan, Ran; Raffanti, Rick; Werthimer, Dan

    2012-04-01

    Microwave kinetic inductance detectors (MKIDs) are superconducting detectors capable of counting single photons and measuring their energy in the UV, optical, and near-IR. MKIDs feature intrinsic frequency domain multiplexing (FDM) at microwave frequencies, allowing the construction and readout of large arrays. Due to the microwave FDM, MKIDs do not require the complex cryogenic multiplexing electronics used for similar detectors, such as transition edge sensors, but instead transfer this complexity to room temperature electronics where they present a formidable signal processing challenge. In this paper, we describe the first successful effort to build a readout for a photon counting optical/near-IR astronomical instrument, the ARray Camera for Optical to Near-infrared Spectrophotometry. This readout is based on open source hardware developed by the Collaboration for Astronomy Signal Processing and Electronics Research. Designed principally for radio telescope backends, it is flexible enough to be used for a variety of signal processing applications.

  19. A readout for large arrays of microwave kinetic inductance detectors.

    PubMed

    McHugh, Sean; Mazin, Benjamin A; Serfass, Bruno; Meeker, Seth; O'Brien, Kieran; Duan, Ran; Raffanti, Rick; Werthimer, Dan

    2012-04-01

    Microwave kinetic inductance detectors (MKIDs) are superconducting detectors capable of counting single photons and measuring their energy in the UV, optical, and near-IR. MKIDs feature intrinsic frequency domain multiplexing (FDM) at microwave frequencies, allowing the construction and readout of large arrays. Due to the microwave FDM, MKIDs do not require the complex cryogenic multiplexing electronics used for similar detectors, such as transition edge sensors, but instead transfer this complexity to room temperature electronics where they present a formidable signal processing challenge. In this paper, we describe the first successful effort to build a readout for a photon counting optical/near-IR astronomical instrument, the ARray Camera for Optical to Near-infrared Spectrophotometry. This readout is based on open source hardware developed by the Collaboration for Astronomy Signal Processing and Electronics Research. Designed principally for radio telescope backends, it is flexible enough to be used for a variety of signal processing applications. PMID:22559560

  20. SNO+ Readout Electronics Upgrades

    NASA Astrophysics Data System (ADS)

    Bonventre, Richard; Shokair, Timothy; Knapik, Robert

    2012-03-01

    The SNO+ experiment is designed to explore several topics in neutrino physics including neutrinoless double beta decay, reactor antineutrinos, and low energy solar neutrinos. SNO+ uses the existing Sudbury Neutrino Observatory (SNO) detector, with the heavy water target replaced with liquid scintillator. The new target requires an upgrade to the command and control electronics to handle the higher rates expected with scintillation light as compared to Cherenkov light. The readout electronics have been upgraded to autonomously push data to a central data acquisition computer over ethernet from each of the 19 front end crates. The autonomous readout is achieved with a field programmable gate array (FPGA) with an embedded processor. Inside the FPGA fabric a state machine is configured to pull data across the VME-like bus of each crate. A small C program, making use of the open source Light Weight IP (LWIP) libraries, is run directly on the hardware (with no operating system) to push the data via TCP/IP. The hybrid combination of `high-level' C code and a `low-level' VHDL state machine is a cost effective and flexible solution for reading out individual front end crates.

  1. Spatial-phase code-division multiple-access system with multiplexed Fourier holography switching for reconfigurable optical interconnection.

    PubMed

    Takasago, K; Takekawa, M; Shirakawa, A; Kannari, F

    2000-05-10

    A new, to our knowledge, space-variant optical interconnection system based on a spatial-phase code-division multiple-access technique with multiplexed Fourier holography is described. In this technique a signal beam is spread over wide spatial frequencies by an M-sequence pseudorandom phase code. At a receiver side a selected signal beam is properly decoded, and at the same time its spatial pattern is shaped with a Fourier hologram, which is recorded by light that is encoded with the same M-sequence phase mask as the desired signal beam and by light whose spatial beam pattern is shaped to a signal routing pattern. Using the multiplexed holography, we can simultaneously route multisignal flows into individually specified receiver elements. The routing pattern can also be varied by means of switching the encoding phase code or replacing the hologram. We demonstrated a proof-of-principle experiment with a doubly multiplexed hologram that enables simultaneous routing of two signal beams. Using a numerical model, we showed that the proposed scheme can manage more than 250 routing patterns for one signal flow with one multiplexed hologram at a signal-to-noise ratio of ~5.

  2. Systemic risk in multiplex networks with asymmetric coupling and threshold feedback

    NASA Astrophysics Data System (ADS)

    Burkholz, Rebekka; Leduc, Matt V.; Garas, Antonios; Schweitzer, Frank

    2016-06-01

    We study cascades on a two-layer multiplex network, with asymmetric feedback that depends on the coupling strength between the layers. Based on an analytical branching process approximation, we calculate the systemic risk measured by the final fraction of failed nodes on a reference layer. The results are compared with the case of a single layer network that is an aggregated representation of the two layers. We find that systemic risk in the two-layer network is smaller than in the aggregated one only if the coupling strength between the two layers is small. Above a critical coupling strength, systemic risk is increased because of the mutual amplification of cascades in the two layers. We even observe sharp phase transitions in the cascade size that are less pronounced on the aggregated layer. Our insights can be applied to a scenario where firms decide whether they want to split their business into a less risky core business and a more risky subsidiary business. In most cases, this may lead to a drastic increase of systemic risk, which is underestimated in an aggregated approach.

  3. A versatile-deployable bacterial detection system for food and environmental safety based on LabTube-automated DNA purification, LabReader-integrated amplification, readout and analysis.

    PubMed

    Hoehl, Melanie M; Bocholt, Eva Schulte; Kloke, Arne; Paust, Nils; von Stetten, Felix; Zengerle, Roland; Steigert, Juergen; Slocum, Alexander H

    2014-06-01

    Contamination of foods is a public health hazard that episodically causes thousands of deaths and sickens millions worldwide. To ensure food safety and quality, rapid, low-cost and easy-to-use detection methods are desirable. Here, the LabSystem is introduced for integrated, automated DNA purification, amplification and detection. It consists of a disposable, centrifugally driven DNA purification platform (LabTube) and a low-cost UV/vis-reader (LabReader). For demonstration of the LabSystem in the context of food safety, purification of Escherichia coli (non-pathogenic E. coli and pathogenic verotoxin-producing E. coli (VTEC)) in water and milk and the product-spoiler Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice was integrated and optimized in the LabTube. Inside the LabReader, the purified DNA was amplified, readout and analyzed using both qualitative isothermal loop-mediated DNA amplification (LAMP) and quantitative real-time PCR. For the LAMP-LabSystem, the combined detection limits for purification and amplification of externally lysed VTEC and A. acidoterrestris are 10(2)-10(3) cell-equivalents. In the PCR-LabSystem for E. coli cells, the quantification limit is 10(2) cell-equivalents including LabTube-integrated lysis. The demonstrated LabSystem only requires a laboratory centrifuge (to operate the disposable, fully closed LabTube) and a low-cost LabReader for DNA amplification, readout and analysis. Compared with commercial DNA amplification devices, the LabReader improves sensitivity and specificity by the simultaneous readout of four wavelengths and the continuous readout during temperature cycling. The use of a detachable eluate tube as an interface affords semi-automation of the LabSystem, which does not require specialized training. It reduces the hands-on time from about 50 to 3 min with only two handling steps: sample input and transfer of the detachable detection tube.

  4. Multiplexed Optical Fiber Sensors for Coal Fired Advanced Fossil Energy Systems

    SciTech Connect

    Wang, Anbo; Pickrell, Gary

    2012-03-31

    This report summarizes technical progress on the program Multiplexed Optical Fiber Sensors for Coal Fired Advanced Fossil Energy Systems funded by the National Energy Technology Laboratory of the U.S. Department of Energy, and performed jointly by the Center for Photonics Technology of the Bradley Department of Electrical and Computer Engineering and the Department of Materials Science and Engineering at Virginia Tech. This three-year project started on October 1, 2008. In the project, a fiber optical sensing system based on intrinsic Fabry-Perot Interferometer (IFPI) was developed for strain and temperature measurements for Ultra Supercritical boiler condition assessment. Investigations were focused on sensor design, fabrication, attachment techniques and novel materials for high temperature and strain measurements. At the start of the project, the technical requirements for the sensing technology were determined together with our industrial partner Alstom Power. As is demonstrated in Chapter 4, all the technical requirements are successfully met. The success of the technology extended beyond laboratory test; its capability was further validated through the field test at DOE NETL, in which the sensors yielded distributed temperature mapping of a testing coupon installed in the turbine test rig. The measurement results agreed well with prior results generated with thermocouples. In this project, significant improvements were made to the IFPI sensor technology by splicing condition optimization, transmission loss reduction, sensor signal demodulation and sensor system design.

  5. Improved polarization dependent loss tolerance for polarization multiplexed coherent optical systems by polarization pairwise coding.

    PubMed

    Zhu, Chen; Song, Binhuang; Corcoran, Bill; Zhuang, Leimeng; Lowery, Arthur James

    2015-10-19

    Polarization dependent loss (PDL) causes imbalanced optical signal to noise ratio (OSNR) of the two polarizations, thus remains one of the major bottlenecks for next-generation polarization-division-multiplexed (PDM) coherent optical transmission systems. In this paper, we investigate Pairwise Coding for adaptive PDL mitigation in PDM coherent optical systems. By pre-coding across two polarizations, the PDL-induced performance degradation can be largely mitigated without any coding overhead. We present details of the coding and de-coding design, and also derive the analytical symbol/bit error rate of the Polarization Pairwise Coding scheme, which can be used to predict the performance gain as well as for optimal rotation angle calculation. Simulation results verify that Pairwise Coding achieves substantial system performance gains over a wide range of PDL values. Compared with other digital coding techniques, Polarization Pairwise Coding shows improved performance than Walsh-Hadamard transform since it maximizes the coordinate diversity; and also Pairwise Coding is computationally much simpler to decode compared with the Golden and Silver Codes, therefore is practical for current 100-Gb/s and future 400-Gb/s and 1-Tb/s digital coherent transceivers. PMID:26480404

  6. Improved superimposed training sequence-based timing synchronization for space optical orthogonal frequency division multiplexing system

    NASA Astrophysics Data System (ADS)

    Wang, Ruyan; Wang, Xiaobing; Zhao, Hui

    2015-10-01

    This paper investigates the timing synchronization problem of a space optical orthogonal frequency division multiplexing (OOFDM) communication system. First, based on the good autocorrelation property of generalized chirp-like sequence, a training sequence is constructed to fit the non-negative light intensity signal requirement of the OOFDM system, of which the front and rear portions are cyclical and the whole is mirror-symmetric. No longer a periodic-repetition structure, the mirror-symmetric structure can effectively avoid the side lobe of objective function and reduce the complexity of correlation calculation, and thereby can improve the synchronization performance. Then, the constructed training sequence is superimposed on a complete data symbol for transmission to efficiently utilize transmitting power and spectrum resources of the communication system. At the receiver, the position of timing synchronization is estimated using maximum-likelihood algorithm and the correlation between the local sequence and the received signal. Simulation results show that, compared with several existing methods, the proposed timing synchronization method achieves better synchronization performances under both strong and weak atmospheric turbulence channels.

  7. A low power readout circuit approach for uncooled resistive microbolometer FPAs

    NASA Astrophysics Data System (ADS)

    Tepegoz, Murat; Toprak, Alperen; Akin, Tayfun

    2008-04-01

    This paper presents a new, low power readout circuit approach for uncooled resistive microbolometer FPAs. The readout circuits of the microbolometer detectors contain parallel readout channels whose outputs are driven and multiplexed on large bus capacitances in order to form the output of the readout circuit. High number of opamps used in the readout channel array and large output capacitances that these opamps should drive necessitates the use of high output current capacity structures, which results in large power dissipation. This paper proposes two new methods in order to decrease the power dissipation of the readout circuits for uncooled thermal FPAs. The first method is called the readout channel group concept, where the readout channel array is separated into groups in order to decrease the load capacitance seen by the readout channel output. The second method utilizes a special opamp architecture where the output current driving capacity can be digitally controlled. This method enables efficient use of power by activating the high output current driving capacity only during the output multiplexing. The simulations show that using these methods results in a power dissipation reduction of 80% and 91% for the readout channels optimized for a single output 384x288 FPA operating at 25 fps and for a two-output 640x480 FPA operating at 30 fps, respectively.

  8. A data acquisition system for two-dimensional position sensitive micropattern gas detectors with delay-line readout

    NASA Astrophysics Data System (ADS)

    Hanu, A. R.; Prestwich, W. V.; Byun, S. H.

    2015-04-01

    We present a data acquisition (DAQ) system for two-dimensional position sensitive micropattern gas detectors using the delay-line method for readout. The DAQ system consists of a field programmable gate array (FPGA) as the main data processor and our time-to-digital (TDC) mezzanine card for making time measurements. We developed the TDC mezzanine card around the Acam TDC-GPX ASIC and it features four independent stop channels referenced to a common start, a typical timing resolution of ~81 ps, and a 17-bit measurement range, and is compliant with the VITA 57.1 standard. For our DAQ system, we have chosen the Xilinx SP601 development kit which features a single Spartan 6 FPGA, 128 MB of DDR2 memory, and a serial USB interface for communication. Output images consist of 1024×1024 square pixels, where each pixel has a 32-bit depth and corresponds to a time difference of 162 ps relative to its neighbours. When configured for a 250 ns acquisition window, the DAQ can resolve periodic event rates up to 1.8×106 Hz without any loses and will report a maximum event rate of 6.11×105 Hz for events whose arrival times follow Poisson statistics. The integral and differential non-linearities have also been measured and are better than 0.1% and 1.5%, respectively. Unlike commercial units, our DAQ system implements the delay-line image reconstruction algorithm entirely in hardware and is particularly attractive for its modularity, low cost, ease of integration, excellent linearity, and high throughput rate.

  9. Increment of Access Points in Integrated System of Wavelength Division Multiplexed Passive Optical Network Radio over Fiber.

    PubMed

    Amiri, I S; Alavi, S E; Soltanian, M R K; Fisal, N; Supa'at, A S M; Ahmad, H

    2015-01-01

    This paper describes a novel technique to increase the numbers of access points (APs) in a wavelength division multiplexed-passive optical network (WDM-PON) integrated in a 100 GHz radio-over-fiber (RoF). Eight multi-carriers separated by 25 GHz intervals were generated in the range of 193.025 to 193.200 THz using a microring resonator (MRR) system incorporating an add-drop filter system. All optically generated multi-carriers were utilized in an integrated system of WDM-PON-RoF for transmission of four 43.6 Gb/sec orthogonal frequency division multiplexing (OFDM) signals. Results showed that an acceptable BER variation for different path lengths up to 25 km was achievable for all four access points and thus the transmission of four OFDM channels is feasible for a 25 km standard single mode fiber (SSMF) path length. PMID:26153536

  10. High-resolution gamma-ray spectroscopy with a microwave-multiplexed transition-edge sensor array

    SciTech Connect

    Noroozian, Omid; Mates, John A. B.; Bennett, Douglas A.; Brevik, Justus A.; Fowler, Joseph W.; Gao, Jiansong; Hilton, Gene C.; Horansky, Robert D.; Irwin, Kent D.; Schmidt, Daniel R.; Vale, Leila R.; Ullom, Joel N.; Kang, Zhao

    2013-11-11

    We demonstrate very high resolution photon spectroscopy with a microwave-multiplexed two-pixel transition-edge sensor (TES) array. We measured a {sup 153}Gd photon source and achieved an energy resolution of 63 eV full-width-at-half-maximum at 97 keV and an equivalent readout system noise of 86 pA/√(Hz) at the TES. The readout circuit consists of superconducting microwave resonators coupled to radio-frequency superconducting-quantum-interference-devices and transduces changes in input current to changes in phase of a microwave signal. We use flux-ramp modulation to linearize the response and evade low-frequency noise. This demonstration establishes one path for the readout of cryogenic X-ray and gamma-ray sensor arrays with more than 10{sup 3} elements and spectral resolving powers R=λ/Δλ>10{sup 3}.

  11. Development of the quality control system of the readout electronics for the large size telescope of the Cherenkov Telescope Array observatory

    NASA Astrophysics Data System (ADS)

    Konno, Y.; Kubo, H.; Masuda, S.; Paoletti, R.; Poulios, S.; Rugliancich, A.; Saito, T.

    2016-07-01

    The Cherenkov Telescope Array (CTA) is the next generation VHE γ-ray observatory which will improve the currently available sensitivity by a factor of 10 in the range 100 GeV to 10 TeV. The array consists of different types of telescopes, called large size telescope (LST), medium size telescope (MST) and small size telescope (SST). A LST prototype is currently being built and will be installed at the Observatorio Roque de los Muchachos, island of La Palma, Canary islands, Spain. The readout system for the LST prototype has been designed and around 300 readout boards will be produced in the coming months. In this note we describe an automated quality control system able to measure basic performance parameters and quickly identify faulty boards.

  12. Towards a multi-channel TOF-PET system with SiPM readout

    NASA Astrophysics Data System (ADS)

    Garutti, Erika; Göttlich, Martin; Harion, Tobias; Hegemann, Niklas; Schmidt, Maximilian; Schultz-Coulon, Hans-Christian; Shen, Wei; Silenzi, Alessandro; Stamen, Rainer; Tadday, Alexander; Xu, Chen

    2012-12-01

    The goal of this project is to develop a multi-channel TOF-PET system with a 300 ps FWHM time resolution, a factor two improvement with respect to commercially available systems (Surti et al., 2007 [1]). In a TOF-PET system, the time-of-flight information can be used to improve significantly the sensitivity of the detector as shown in Karp et al. (2008) [2]. The target time resolution has been achieved in two channel systems with LYSO (Kim and Wang, 2008 [3]), the aim is to port this results into a multi-channel system. This work extends the results shown in Göttlich et al. (2010) [4], studying the stability of the detector performance in different geometries and configurations.

  13. Electra: durable repetitively pulsed angularly multiplexed KrF laser system

    NASA Astrophysics Data System (ADS)

    Wolford, Matthew F.; Myers, Matthew C.; Giuliani, John L.; Sethian, John D.; Burns, Patrick M.; Hegeler, Frank; Jaynes, Reginald

    2008-02-01

    Electra is a repetitively pulsed, electron beam pumped Krypton Fluoride (KrF) laser at the Naval Research Laboratory that is developing the technologies that can meet the Inertial Fusion Energy (IFE) requirements for durability, efficiency, and cost. The technologies developed on Electra should be directly scalable to a full size fusion power plant beam line. As in a full size fusion power plant beam line, Electra is a multistage laser amplifier system which, consists of a commercial discharge laser (LPX 305i, Lambda Physik), 175 keV electron beam pumped (40 ns flat-top) preamplifier, and 530 keV (100 ns flat-top) main amplifier. Angular multiplexing is used in the optical layout to provide pulse length control and to maximize laser extraction from the amplifiers. Single shot yield of 452 J has been extracted from the initial shots of the Electra laser system using a relatively low energy preamplifier laser beam. In rep-rate burst of 5 Hz for durations of one second a total energy of 1.585 kJ (average 317 J/pulse) has been attained. Total energy of 2.5 kJ has been attained over a two second period. For comparison, the main amplifier of Electra in oscillator mode has demonstrated at 2.5 Hz rep-rate average laser yield of 270 J over a 2 hour period.

  14. Self-calibrating multiplexer circuit

    DOEpatents

    Wahl, Chris P.

    1997-01-01

    A time domain multiplexer system with automatic determination of acceptable multiplexer output limits, error determination, or correction is comprised of a time domain multiplexer, a computer, a constant current source capable of at least three distinct current levels, and two series resistances employed for calibration and testing. A two point linear calibration curve defining acceptable multiplexer voltage limits may be defined by the computer by determining the voltage output of the multiplexer to very accurately known input signals developed from predetermined current levels across the series resistances. Drift in the multiplexer may be detected by the computer when the output voltage limits, expected during normal operation, are exceeded, or the relationship defined by the calibration curve is invalidated.

  15. A drift detector system with anode and cathode readout in the GlueX experiment

    SciTech Connect

    Berdnikov, V V; Somov, S V; Pentchev, L; Zihlmann, B

    2015-01-01

    A drift detector system designed to detect charged particle tracks in the GlueX experiment dedicated to study the nature of confinement is described. The key design features of the drift chambers associated with the requirement of a minimum material budget in the path of secondary particles are presented. The spatial resolution and the detection efficiency have been measured with cosmic rays using the automatic data acquisition system.

  16. The readout chain for the bar PANDA MVD strip detector

    NASA Astrophysics Data System (ADS)

    Schnell, R.; Brinkmann, K.-Th.; Di Pietro, V.; Kleines, H.; Goerres, A.; Riccardi, A.; Rivetti, A.; Rolo, M. D.; Sohlbach, H.; Zaunick, H.-G.

    2015-02-01

    The bar PANDA (antiProton ANnihilation at DArmstadt) experiment will study the strong interaction in annihilation reactions between an antiproton beam and a stationary gas jet target. The detector will comprise different sub-detectors for tracking, particle identification and calorimetry. The Micro-Vertex Detector (MVD) as the innermost part of the tracking system will allow precise tracking and detection of secondary vertices. For the readout of the double-sided silicon strip sensors a custom-made ASIC is being developed, employing the Time-over-Threshold (ToT) technique for digitization and utilize time-to-digital converters (TDC) to provide a high-precision time stamp of the hit. A custom-made Module Data Concentrator ASIC (MDC) will multiplex the data of all front-ends of one sensor towards the CERN-developed GBT chip set (GigaBit Transceiver). The MicroTCA-based MVD Multiplexer Board (MMB) at the off-detector site will receive and concentrate the data from the GBT links and transfer it to FPGA-based compute nodes for global event building.

  17. Development of a High Dynamic Range Read-out System Using Multi-photodiode for the Total Absorption Calorimeter of CALET

    NASA Astrophysics Data System (ADS)

    Katayose, Y.; Shibata, M.; Torii, S.; Shimizu, Y.; Tamura, T.; Hibino, K.; Okuno, S.; Yoshida, K.; Kitamura, H.; Uchihori, Y.; Murakami, H.

    We have been developing the CALET instrument, which is proposed to be launched on the Japanese Experiment Module (JEM), Exposed Facility (EF) of the ISS. CALET consists of an imaging calorimeter (IMC) and a total absorption calorimeter (TASC). The role of IMC is identification of the incident particle by imaging the shower tracks with scintillating fibers. TASC is used for observing the total development of shower particles with a stack of BGO scintillators. A read-out system using multi-photodiode and a front-end circuit including analog ASIC, 16 bit ADC, FPGA was developed to measure the energy deposit with the dynamic range from 1MIP(Minimum Ionization Particle) up to 106MIPs in a BGO bar of TASC. The output signal of 1 MIP was calibrated by cosmic ray muon. The dynamic range of the read-out system was measured with both LED pulser and heavy ions beam in the range from 1MIP to about 2400 MIPs . In this paper, the performance of the read-out system is described.

  18. Population data on 10 non-CODIS STR loci in Japanese population using a newly developed multiplex PCR system.

    PubMed

    Asamura, H; Ota, M; Fukushima, H

    2008-11-01

    This paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 10 loci (D1S1656, D2S1353, D8S1132, D12S1090, D14S608, D18S535, D19S253, D20S480, D21S226, and D22S689) unlinked to the core STR loci (non-CODIS loci). Of 252 samples taken from the Japanese population, PCR products ranged in length from 107 bp to 319 bp. No significant deviations from Hardy-Weinberg equilibrium were observed at any of the 10 loci. The accumulated power of discrimination and power of exclusion for the 10 loci were 0.999999999998 and 0.99991, respectively. We conclude that the present multiplex system for the 10 non-CODIS loci represents a powerful tool for forensic applications.

  19. Effectiveness of phase-conjugated twin waves on fiber nonlinearity in spatially multiplexed all-optical OFDM system

    NASA Astrophysics Data System (ADS)

    Hmood, Jassim K.; Noordin, Kamarul A.; Harun, Sulaiman W.

    2016-07-01

    In this paper, we investigate the effectiveness of using phase-conjugated twin waves (PCTWs) technique to mitigate fiber nonlinear impairments in spatially multiplexed all-optical orthogonal frequency division multiplexing (AO-OFDM) systems. In this technique, AO-OFDM signal and its phase-conjugated copy are directly transmitted through two identical fiber links. At the receiver, the two signals are coherently superimposed to cancel the phase noise and to enhance signal-to-noise ratio (SNR). To show the effectiveness of proposed technique, a spatially multiplexed AO-OFDM system is demonstrated by numerical simulation. AO-OFDM signal and its phase conjugated copy are optically generated by using optical coupler-based inverse fast Fourier transform (OIFFT)/fast Fourier transform (OFFT). The generated signal includes 29 subcarriers where each subcarrier is modulated by 4-quadrature amplitude modulation (4QAM) format at a symbol rate of 25 Gsymbol/s. The results reveal that transmission performance is considerably improved where the transmission distance of the proposed system is increased by ∼45% as compared to that of original system without PCTWs technique.

  20. Designing multiplex PCR system of Campylobacter jejuni for efficient typing by improving monoplex PCR binary typing method.

    PubMed

    Yamada, Kazuhiro; Ibata, Ami; Suzuki, Masahiro; Matsumoto, Masakado; Yamashita, Teruo; Minagawa, Hiroko; Kurane, Ryuichiro

    2015-01-01

    Campylobacter jejuni is responsible for the majority of Campylobacter infections. As the molecular epidemiological study of outbreaks, pulsed-field gel electrophoresis (PFGE) is performed in general. But PFGE has several problems. PCR binary typing (P-BIT) method is a typing method for Campylobacter spp. that was recently developed, and was reported to have a similar discriminatory power and stability to those of PFGE. We modified the P-BIT method from 18 monoplex PCRs to two multiplex PCR systems (mP-BIT). The same results were obtained from monoplex PCRs using original primers and multiplex PCR in the representative isolates. The mP-BIT can analyze 48 strains at a time by using 96-well PCR systems and can identify C. jejuni because mP-BIT includes C. jejuni marker. The typing of the isolates by the mP-BIT and PFGE demonstrated generally concordant results and the mP-BIT method (D = 0.980) has a similar discriminatory power to that of PFGE with SmaI digest (D = 0.975) or KpnI digest (D = 0.987) as with original article. The mP-BIT method is quick, simple and easy, and comes to be able to perform it at low cost by having become a multiplex PCR system. Therefore, the mP-BIT method with two multiplex PCR systems has high potential for a rapid first-line surveillance typing assay of C. jejuni and can be used for routine surveillance and outbreak investigations of C. jejuni in the future.

  1. Designing multiplex PCR system of Campylobacter jejuni for efficient typing by improving monoplex PCR binary typing method.

    PubMed

    Yamada, Kazuhiro; Ibata, Ami; Suzuki, Masahiro; Matsumoto, Masakado; Yamashita, Teruo; Minagawa, Hiroko; Kurane, Ryuichiro

    2015-01-01

    Campylobacter jejuni is responsible for the majority of Campylobacter infections. As the molecular epidemiological study of outbreaks, pulsed-field gel electrophoresis (PFGE) is performed in general. But PFGE has several problems. PCR binary typing (P-BIT) method is a typing method for Campylobacter spp. that was recently developed, and was reported to have a similar discriminatory power and stability to those of PFGE. We modified the P-BIT method from 18 monoplex PCRs to two multiplex PCR systems (mP-BIT). The same results were obtained from monoplex PCRs using original primers and multiplex PCR in the representative isolates. The mP-BIT can analyze 48 strains at a time by using 96-well PCR systems and can identify C. jejuni because mP-BIT includes C. jejuni marker. The typing of the isolates by the mP-BIT and PFGE demonstrated generally concordant results and the mP-BIT method (D = 0.980) has a similar discriminatory power to that of PFGE with SmaI digest (D = 0.975) or KpnI digest (D = 0.987) as with original article. The mP-BIT method is quick, simple and easy, and comes to be able to perform it at low cost by having become a multiplex PCR system. Therefore, the mP-BIT method with two multiplex PCR systems has high potential for a rapid first-line surveillance typing assay of C. jejuni and can be used for routine surveillance and outbreak investigations of C. jejuni in the future. PMID:25455748

  2. Readout cross-talk for alpha-particle measurements in a pixelated sensor system

    NASA Astrophysics Data System (ADS)

    Norlin, B.; Reza, S.; Krapohl, D.; Fröjdh, E.; Thungström, G.

    2015-05-01

    Simulations in Medici are performed to quantify crosstalk and charge sharing in a hybrid pixelated silicon detector. Crosstalk and charge sharing degrades the spatial and spectral resolution of single photon processing X-ray imaging systems. For typical medical X-ray imaging applications, the process is dominated by charge sharing between the pixels in the sensor. For heavier particles each impact generates a large amount of charge and the simulation seems to over predict the charge collection efficiency. This indicates that some type of non modelled degradation of the charge transport efficiency exists, like the plasma effect where the plasma might shield the generated charges from the electric field and hence distorts the charge transport process. Based on the simulations it can be reasoned that saturation of the amplifiers in the Timepix system might generate crosstalk that increases the charge spread measured from ion impact on the sensor.

  3. Data acquisition and readout system for the LUX dark matter experiment

    NASA Astrophysics Data System (ADS)

    Akerib, D. S.; Bai, X.; Bedikian, S.; Bernard, E.; Bernstein, A.; Bradley, A.; Cahn, S. B.; Carmona-Benitez, M. C.; Carr, D.; Chapman, J. J.; Clark, K.; Classen, T.; Coffey, T.; Curioni, A.; Dazeley, S.; de Viveiros, L.; Dragowsky, M.; Druszkiewicz, E.; Faham, C. H.; Fiorucci, S.; Gaitskell, R. J.; Gibson, K. R.; Hall, C.; Hanhardt, M.; Holbrook, B.; Ihm, M.; Jacobsen, R. G.; Kastens, L.; Kazkaz, K.; Lander, R.; Larsen, N.; Lee, C.; Leonard, D.; Lesko, K.; Lyashenko, A.; Malling, D. C.; Mannino, R.; McKinsey, D. N.; Mei, D.; Mock, J.; Morii, M.; Nelson, H.; Nikkel, J. A.; Pangilinan, M.; Phelps, P.; Shutt, T.; Skulski, W.; Sorensen, P.; Spaans, J.; Stiegler, T.; Svoboda, R.; Sweany, M.; Szydagis, M.; Thomson, J.; Tripathi, M.; Verbus, J. R.; Walsh, N.; Webb, R.; White, J. T.; Wlasenko, M.; Wolfs, F. L. H.; Woods, M.; Zhang, C.

    2012-03-01

    LUX is a two-phase (liquid/gas) xenon time projection chamber designed to detect nuclear recoils from interactions with dark matter particles. Signals from the LUX detector are processed by custom-built analog electronics which provide properly shaped signals for the trigger and data acquisition (DAQ) systems. The DAQ is composed of commercial digitizers with firmware customized for the LUX experiment. Data acquisition systems in rare-event searches must accommodate high rate and large dynamic range during precision calibrations involving radioactive sources, while also delivering low threshold for maximum sensitivity. The LUX DAQ meets these challenges using real-time baseline suppression that allows for a maximum event acquisition rate in excess of 1.5 kHz with virtually no deadtime. This paper describes the LUX DAQ and the novel acquisition techniques employed in the LUX experiment.

  4. A SQUID readout system for a superconducting gyroscope. [superconducting quantum interference device

    NASA Technical Reports Server (NTRS)

    Hendricks, J. B.

    1975-01-01

    A design of a read out system for a superconducting gyroscope to be used in an orbiting gyroscope relativity experiment is discussed. The 'London Moment' of the superconducting rotor, which lies along the spin axis of the rotor, will be measured with a SQUID-type magnetometer. The SQUID will be built around the gyro rotor, with a very close spacing to give an inductance between 10 millionths and 1 millionth Hy. A SQUID of this design should resolve 2.07 times 10 to the minus 19th weber. The angular resolution of the gyroscope will then be 0.0035 arc-second, which is sufficient for the intended experiment.

  5. The read-out system of spatial distribution of thermoluminescence in meteorites

    NASA Technical Reports Server (NTRS)

    Ninagawa, K.; Yamamoto, I.; Takano, Y.; Wada, T.; Yamashita, Y.; Takaoka, N.

    1985-01-01

    The thermoluminescence (TL) technique used for dating the terrestrial age of meteorites is based on the TL fading of interior samples. The depth dependence of the TL for Antarctic meteorites with fusion crust is measured. Usually, meteorites are powdered and their TL measured under a photomultiplier. In this case, a TL spatial distribution of a cross section of antarctic meteorites is measured using a read out system of spatial distribution of TL, since a meteorite is made up of inhomogeneous material. Antarctic meteorites MET-78028(L6) and ALH-77278(L13) are used.

  6. Analog bus driver and multiplexer

    NASA Technical Reports Server (NTRS)

    Pain, Bedabrata (Inventor); Hancock, Bruce (Inventor); Cunningham, Thomas J. (Inventor)

    2012-01-01

    For a source-follower signal chain, the ohmic drop in the selection switch causes unacceptable voltage offset, non-linearity, and reduced small signal gain. For an op amp signal chain, the required bias current and the output noise rises rapidly with increasing the array format due to a rapid increase in the effective capacitance caused by the Miller effect boosting up the contribution of the bus capacitance. A new switched source-follower signal chain circuit overcomes limitations of existing op-amp based or source follower based circuits used in column multiplexers and data readout. This will improve performance of CMOS imagers, and focal plane read-out integrated circuits for detectors of infrared or ultraviolet light.

  7. Multiple layer optical memory system using second-harmonic-generation readout

    DOEpatents

    Boyd, Gary T.; Shen, Yuen-Ron

    1989-01-01

    A novel optical read and write information storage system is described which comprises a radiation source such as a laser for writing and illumination, the radiation source being capable of radiating a preselected first frequency; a storage medium including at least one layer of material for receiving radiation from the radiation source and capable of being surface modified in response to said radiation source when operated in a writing mode and capable of generating a pattern of radiation of the second harmonic of the preselected frequency when illuminated by the radiation source at the preselected frequency corresponding to the surface modifications on the storage medium; and a detector to receive the pattern of second harmonic frequency generated.

  8. Readout process and noise elimination firmware for the Fermilab beam loss system

    SciTech Connect

    Wu, Jinyuan; Baumbaugh, Alan; Drennan, Craig; Thurman-Keup, Randy; Lewis, Jonathan; Shi, Zonghan; /Fermilab

    2007-05-01

    In the Fermilab Beam Loss Monitor System, inputs from ion chambers are integrated for a short period of time, digitized and processed to create the accelerator abort request signals. The accelerator power supplies employing 3-phase 60Hz AC cause noise at various harmonics on our inputs which must be eliminated for monitoring purposes. During accelerator ramping, both the sampling frequency and the amplitudes of the noise components change. As such, traditional digital filtering can partially reduce certain noise components but not all. A nontraditional algorithm was developed in our work to eliminate remaining ripples. The sequencing in the FPGA firmware is conducted by a micro-sequencer core we developed: the Enclosed Loop Micro-Sequencer (ELMS). The unique feature of the ELMS is that it supports the ''FOR'' loops with pre-defined iterations at the machine code level, which provides programming convenience and avoids many micro-complexities from the beginning.

  9. Proposal for Angular Momentum Multiplexing in Microholographic Recording

    NASA Astrophysics Data System (ADS)

    Katayama, Ryuichi

    2013-09-01

    A novel multiplexing technology in microholographic recording using beams that have an orbital angular momentum has been proposed. The multiplexing is carried out by changing the order of the phase singularity (m) of beams for recording and readout in multiple states. In the recording operation, multiple microholograms are formed at the same position of the recording medium by changing the value of m. In the readout operation, each of the multiple microholograms is selectively reproduced by changing the value of m. Microholograms with m≠0 have a spiral shape, and the handednesses, multiplicities, and pitches of the spiral differ from each other depending on the value of m. A readout signal simulation has demonstrated that the multiplexing of at least five bits is feasible. It is expected that a terabyte-order recording capacity will be achieved in microholographic recording by combining this technology with three-dimensional recording technology.

  10. 60-GHz optical/wireless MIMO system integrated with optical subcarrier multiplexing and 2x2 wireless communication.

    PubMed

    Lin, Chi-Hsiang; Lin, Chun-Ting; Huang, Hou-Tzu; Zeng, Wei-Siang; Chiang, Shou-Chih; Chang, Hsi-Yu

    2015-05-01

    This paper proposes a 2x2 MIMO OFDM Radio-over-Fiber scheme based on optical subcarrier multiplexing and 60-GHz MIMO wireless transmission. We also schematically investigated the principle of optical subcarrier multiplexing, which is based on a dual-parallel Mach-Zehnder modulator (DP-MZM). In our simulation result, combining two MIMO OFDM signals to drive DP-MZM gives rise to the PAPR augmentation of less than 0.4 dB, which mitigates nonlinear distortion. Moreover, we applied a Levin-Campello bit-loading algorithm to compensate for the uneven frequency responses in the V-band. The resulting system achieves OFDM signal rates of 61.5-Gbits/s with BER of 10(-3) over 25-km SMF transmission followed by 3-m wireless transmission.

  11. Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    PubMed Central

    Poly, Frédéric; Serichantalergs, Oralak; Kuroiwa, Janelle; Pootong, Piyarat; Mason, Carl; Guerry, Patricia; Parker, Craig T.

    2015-01-01

    Campylobacter jejuni produces a polysaccharide capsule that is the major determinant of the Penner serotyping scheme. This passive slide agglutination typing system was developed in the early 1980’s and was recognized for over two decades as the gold standard for C. jejuni typing. A preliminary multiplex PCR technique covering 17 serotypes was previously developed in order to replace this classic serotyping scheme. Here we report the completion of the multiplex PCR technology that is able to identify all the 47 Penner serotypes types known for C. jejuni. The number of capsule types represented within the 47 serotypes is 35. We have applied this method to a collection of 996 clinical isolates from Thailand, Cambodia and Nepal and were able to successfully determine capsule types of 98% of these. PMID:26630669

  12. R&D on a novel spectro-imaging polarimeter with Micromegas detectors and a Caliste readout system

    NASA Astrophysics Data System (ADS)

    Attié, D.; Blondel, C.; Boilevin-Kayl, L.; Desforges, D.; Ferrer-Ribas, E.; Giomataris, I.; Gevin, O.; Jeanneau, F.; Limousin, O.; Meuris, A.; Papaevangelou, T.; Peyaud, A.

    2015-07-01

    Micromegas detectors, part of the Micro-Pattern Gaseous Detectors (MPGD) family, are used in a very wide range of applications in the High Energy Physics community but also in astroparticle and neutrino physics. In most of the Micromegas applications the design of the detector vessel and the readout plane is extremely coupled. A way of dissociating these two components would be by separating the amplification structure and the detector volume from the readout plane and electronics. This is achieved with the so called piggyback Micromegas detectors. They open up new possibilities of applications in terms of adaptability to new electronics. In particular piggyback resistive Micromegas can be easily coupled to modern pixel array electronic ASICs. First tests have been carried out with a Medipix chip where the protection of the resistive layer has been proved. The results of very recent tests coupling piggyback Micromegas with the readout module of Caliste are presented. Caliste is a high performance spectro-imager with event time-tagging capability, able to detect photons between 2 keV and 250 keV in the context of a spatial micro spectro-imaging polarimetrer. In the current application, with the Piggyback Micromegas, we use the readout module only as the sensitive detector. We benefit of the good spatial resolution thanks to the high density readout pixels (~600 μm pixel pitch), to the low noise, to the low power and to the radiation hard integrated front-end IDEF-X electronics. The advantage of such a device is to have a high gain, low noise, low threshold, and robust detector operating at room temperature. This would be very attractive for spatial applications, for instance X-ray polarisation.

  13. Tests of the standard (30 hz) NCER FM multiplex telemetry system, augmented by two timing channels and a compensation reference signal, used to record multiplexed seismic network data on magnetic tape

    USGS Publications Warehouse

    Eaton, Jerry P.

    1976-01-01

    The application of subtractive compensation to USGS seismic magnetic tape recording and playback systems was examined in a recent USGS Open-file report (1). It was found, for the standard (30 Hz) NCER multiplex system, that subtractive compensation utilizing a 4688 Hz reference signal multiplexed onto each data track was more effective than that utilizing a 3125 Hz reference signal recorded separately on a different track. Moreover, it was found that the portion of the spectrum between the uppermost data channel (3060 Hz + or - 125 Hz) and the compensation reference signal (4688 Hz) could be used to record an additional timing signal, with a center frequency of 3700 Hz and a broader playback bandwidth (ca 0 to 100 Hz) than that of the standard data channels. Accordingly, for the tests described in that report, the standard 8-datachannel multiplex system was augmented by one additional timing channel with a center frequency of 3700 Hz. The 3700 Hz discriminator used in those tests was not successfully set up to utilize subtractive compensation; so its output from a tape playback was quite noisy. Subsequently, further tests have been carried out on the application of subtractive compensation to a 4-channel broad-band multiplex system and to the standard multiplex system, both recorded on field tape recorders with relatively poor tape speed control (2), (3). In the course of these experiments, it was discovered that two separate timing channe1s, not just one, can be inserted between the uppermost data channel and the compensation reference signal, Furthermore, it was possible to adjust the discriminators used to playback these timing channels so that they profited significantly from subtractive compensation even though the playback bandwidth was 0 to 100 Hz (for short rise times of square wave timing signals). The advantages of recording two timing signals on each data track include: 1) one standard time signal to be used for critical timing, e.g. IRIG E, can be

  14. Orbital angular momentum in four channel spatial domain multiplexing system for multi-terabit per second communication architectures

    NASA Astrophysics Data System (ADS)

    Murshid, Syed H.; Muralikrishnan, Hari P.; Kozaitis, Samuel P.

    2012-06-01

    Bandwidth increase has always been an important area of research in communications. A novel multiplexing technique known as Spatial Domain Multiplexing (SDM) has been developed at the Optronics Laboratory of Florida Institute of Technology to increase the bandwidth to T-bits/s range. In this technique, space inside the fiber is used effectively to transmit up to four channels of same wavelength at the same time. Experimental and theoretical analysis shows that these channels follow independent helical paths inside the fiber without interfering with each other. Multiple pigtail laser sources of exactly the same wavelength are used to launch light into a single carrier fiber in a fashion that resulting channels follow independent helical trajectories. These helically propagating light beams form optical vortices inside the fiber and carry their own Orbital Angular Momentum (OAM). The outputs of these beams appear as concentric donut shaped rings when projected on a screen. This endeavor presents the experimental outputs and simulated results for a four channel spatially multiplexed system effectively increasing the system bandwidth by a factor of four.

  15. Y-Short Tandem Repeat Multiplex Systems - Y-PLEX™ 6 and Y-PLEX™ 5.

    PubMed

    Shewale, J G

    2003-07-01

    Y-Chromosome short tandem repeats (Y-STRs) have become a very useful tool in forensic casework, paternity, and male lineage studies. In forensic casework, one can obtain the male profile from a mixture sample containing male and female DNA. Two Y-STR genotyping systems, Y-PLEX™ 6 and Y-PLEX™ 5, have been developed for use in human identification. Y-PLEX™ 6 enables simultaneous amplification of DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385; Y-PLEX™ 5 enables simultaneous amplification of DYS389I, DYS389II, DYS439, DYS438, and DYS392 loci. The Y-PLEX™ 6 and Y-PLEX™ 5 systems together provide analysis of all nine Y-STR loci generating minimal haplotype and two additional loci, DYS438 and DYS439. These systems also provide analysis for all 11 Y-STR loci recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM) for forensic casework and population database studies. Both the systems were validated following the Federal Bureau of Investigation (FBI) Director's Quality Assurance Standards. Allelic ladders, which serve as a reference in genotyping, were generated. The nucleotide sequence of alleles in the allelic ladder was determined and the nomenclature is in accord with the recommendations of the International Society of Forensic Genetics (ISFG). The minimum sensitivity of the Y-PLEX™ 6 and Y-PLEX™ 5 systems was 0.2 and 0.1 ng of male DNA, respectively. The nonhuman study revealed that the primers in the Y-PLEX™ 6 and Y-PLEX™ 5 systems were specific for the DNA from humans and some higher primates. Mean stutter values ranged from 3.6 to 11.9%. The Y-PLEX™ 6 and Y-PLEX™ 5 systems were used in several forensic cases. The results from these multiplex systems have been admitted in various U.S. Courts. Thus, Y-PLEX™ 6 and Y-PLEX™ 5 genotyping systems are sensitive, reliable, and robust for use in human forensic and male lineage identification studies. PMID:26256728

  16. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA

    NASA Astrophysics Data System (ADS)

    Frampton, John P.; White, Joshua B.; Simon, Arlyne B.; Tsuei, Michael; Paczesny, Sophie; Takayama, Shuichi

    2014-05-01

    Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because the detection antibody solutions are co-localized only to corresponding surface-immobilized capture antibody spots. This multiplexing technique is validated using plasma samples from allogeneic bone marrow recipients. Patients with acute graft versus host disease (GVHD), a common and serious condition associated with allogeneic bone marrow transplantation, display higher mean concentrations for four multiplexed biomarkers (HGF, elafin, ST2 and TNFR1) relative to healthy donors and transplant patients without GVHD. The antibody co-localization capability of this technology is particularly useful when using inherently cross-reactive reagents such as polyclonal antibodies, although monoclonal antibody cross-reactivity can also be reduced. Because ATPS-ELISA adapts readily available antibody reagents, plate materials and detection instruments, it should be easily transferable into other research and clinical settings.

  17. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA

    PubMed Central

    Frampton, John P.; White, Joshua B.; Simon, Arlyne B.; Tsuei, Michael; Paczesny, Sophie; Takayama, Shuichi

    2014-01-01

    Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because the detection antibody solutions are co-localized only to corresponding surface-immobilized capture antibody spots. This multiplexing technique is validated using plasma samples from allogeneic bone marrow recipients. Patients with acute graft versus host disease (GVHD), a common and serious condition associated with allogeneic bone marrow transplantation, display higher mean concentrations for four multiplexed biomarkers (HGF, elafin, ST2 and TNFR1) relative to healthy donors and transplant patients without GVHD. The antibody co-localization capability of this technology is particularly useful when using inherently cross-reactive reagents such as polyclonal antibodies, although monoclonal antibody cross-reactivity can also be reduced. Because ATPS-ELISA adapts readily available antibody reagents, plate materials and detection instruments, it should be easily transferable into other research and clinical settings. PMID:24786974

  18. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 7: Specification for EOS low cost readout station

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The functional, performance, and design requirements for the Low Cost Readout Station (LCRS) which supports the Earth Observatory Satellite (EOS) data system are described. The basic LCRS consists of all hardware and software needed to acquire and track the EOS-A or EOS-B satellite and receive, record, process, and annotate the instrument data from the satellites. The LCRS also provides appropriate interfaces with the unique local user provided display and extractive processing equipment. The LCRS has the capability of acquiring image data from the EOS-A and the EOS-B satellites over a ground area defines by a 500 kilometer radius from the coordinates of the station. The LCRS is also capable of receiving and processing both, but not simultaneously, full five band Multispectral Scanner (MSS) image data and various modes of the Compacted Thematic (CTM) data.

  19. Construction of two fluorescence-labeled non-combined DNA index system miniSTR multiplex systems to analyze degraded DNA samples in the Chinese Han Population.

    PubMed

    Bai, Xue; Li, Shujin; Cong, Bin; Li, Xia; Guo, Xia; He, Lujun; Ye, Jian; Pei, Li

    2010-09-01

    MiniSTR loci have been demonstrated to be an effective approach in recovering genetic information from degraded specimens, because of the reduced PCR amplicon sizes which improved the PCR efficiency. Eight non-combined DNA index system miniSTR loci suitable for the Chinese Han Population were analyzed in 300 unrelated Chinese Han individuals using two novel five fluorescence-labeled miniSTR multiplex systems(multiplex I: D10S1248, D2S441, D1S1677 and D9S2157; multiplex II: D9S1122, D10S1435, D12ATA63, D2S1776 and Amelogenin). The allele frequency distribution and forensic parameters in the Chinese Han Population were reported in this article. The Exact Test demonstrated that all loci surveyed here were found to be no deviation from Hardy-Weinberg equilibrium. The accumulated power of discrimination and power of exclusion for the eight loci were 0.999999992 and 0.98, respectively. The highly degraded DNA from artificially degraded samples and the degraded forensic case work samples was assessed with the two miniSTR multiplex systems, and the results showed that the systems were quite effective.

  20. Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens.

    PubMed

    Jääskeläinen, Anne J; Viitala, Sari M; Kurkela, Satu; Hepojoki, Satu; Sillanpää, Heidi; Kallio-Kokko, Hannimari; Bergström, Tomas; Suni, Jukka; Närvänen, Ale; Vapalahti, Olli; Vaheri, Antti

    2014-05-01

    Central nervous system (CNS) infections have multiple potential causative agents for which simultaneous pathogen screening can provide a useful tool. This study evaluated a multiplexed microarray for the simultaneous detection of antibodies against CNS pathogens. The performance of selected microarray antigens for the detection of IgG antibodies against herpes simplex virus 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), adenovirus, Mycoplasma pneumoniae and Borrelia burgdorferi sensu lato, was evaluated using serum sample panels tested with reference assays used in a routine diagnostic laboratory. The microarray sensitivity for HSV-1, HSV-2, VZV, adenovirus and M. pneumonia ranged from 77% to 100%, and the specificity ranged from 74% to 97%. Very variable sensitivities and specificities were found for borrelial antigens of three different VlsE protein IR(6) peptide variants (IR6p1, IR6p2, IR6p4) and three recombinant decorin binding proteins A (DbpA; DbpAIa, DbpA91, DbpAG40). For single antigens, good specificity was shown for antigens of IR6p4 and DbpAIa (96%), while DbpA91, IR6p1 and IR6p2 were moderately specific (88-92%). The analytical sensitivity of the microarray was dependent on the borrelial IgG concentration of the specimen. The overall performance and technical features of the platform showed that the platform supports both recombinant proteins, whole viruses and peptides as antigens. This study showed diagnostic potential for all six CNS pathogens, including Borrelia burgdorferi sensu lato, using glutaraldehyde based microarray, and further highlighted the importance of careful antigen selection and the requirement for the use of multiple borrelial antigens in order to increase specificity without a major lack of sensitivity.

  1. A multiplexed quantum memory.

    PubMed

    Lan, S-Y; Radnaev, A G; Collins, O A; Matsukevich, D N; Kennedy, T A; Kuzmich, A

    2009-08-01

    A quantum repeater is a system for long-distance quantum communication that employs quantum memory elements to mitigate optical fiber transmission losses. The multiplexed quantum memory (O. A. Collins, S. D. Jenkins, A. Kuzmich, and T. A. B. Kennedy, Phys. Rev. Lett. 98, 060502 (2007)) has been shown theoretically to reduce quantum memory time requirements. We present an initial implementation of a multiplexed quantum memory element in a cold rubidium gas. We show that it is possible to create atomic excitations in arbitrary memory element pairs and demonstrate the violation of Bell's inequality for light fields generated during the write and read processes.

  2. Development of a 20-locus fluorescent multiplex system as a valuable tool for national DNA database.

    PubMed

    Jiang, Xianhua; Guo, Fei; Jia, Fei; Jin, Ping; Sun, Zhu

    2013-02-01

    The multiplex system allows the detection of 19 autosomal short tandem repeat (STR) loci [including all Combined DNA Index System (CODIS) STR loci as well as D2S1338, D6S1043, D12S391, D19S433, Penta D and Penta E] plus the sex-determining locus Amelogenin in a single reaction, comprising all STR loci in various commercial kits used in the China national DNA database (NDNAD). Primers are designed so that the amplicons are distributed ranging from 90 base pairs (bp) to 450 bp within a five-dye fluorescent design with the fifth dye reserved for the internal size standard. With 30 cycles, 125 pg to 2 ng DNA template showed optimal profiling result, while robust profiles could also be achieved by adjusting the cycle numbers for the DNA template beyond that optimal DNA input range. Mixture studies showed that 83% and 87% of minor alleles were detected at 9:1 and 1:9 ratios, respectively. When 4 ng of degraded DNA was digested by 2-min DNase and 1 ng undegraded DNA was added to 400 μM haematin, the complete profiles were still observed. Polymerase chain reaction (PCR)-based procedures were examined and optimized including the concentrations of primer set, magnesium and the Taq polymerase as well as volume, cycle number and annealing temperature. In addition, the system has been validated by 3000 bloodstain samples and 35 common case samples in line with the Chinese National Standards and Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The total probability of identity (TPI) can reach to 8×10(-24), where DNA database can be improved at the level of 10 million DNA profiles or more because the number of expected match is far from one person (4×10(-10)) and can be negligible. Further, our system also demonstrates its good performance in case samples and it will be an ideal tool for forensic DNA typing and databasing with potential application.

  3. Superconducting THz Camera with GaAs-JFET Cryogenic Readout Electronics

    NASA Astrophysics Data System (ADS)

    Matsuo, Hiroshi; Hibi, Yasunori; Suzuki, Toyoaki; Naruse, Masato; Noguchi, Takashi; Sekimoto, Yutaro; Uzawa, Yoshinori; Nagata, Hirohisa; Ikeda, Hirokazu; Ariyoshi, Seiichiro; Otani, Chiko; Nitta, Tom; Qi-jun, Yao, Fujiwara, Mikio

    2009-12-01

    We describe the development of large format array of superconducting tunnel junction detectors that is readout by SONY GaAs-JFET cryogenic integrated circuits. High quality SIS photon detectors have high dynamic impedance that can be readout by low gate leakage GaAs-JFET circuits. Our imaging array design, with niobium SIS photon detectors and GaAs-JFET cryogenics electronics, uses integrating amplifiers, multiplexers and shift-registers to readout large number of pixels that is similar to CMOS digital cameras. We have designed and fabricated GaAs-JFET cryogenic integrated circuits, such as AC-coupled capacitive trans-impedance amplifier, multiplexers with sample-and-holds and shift-registers, for 32-channel readout module. The Advanced Technology Center of National Astronomical Observatory of Japan have started extensive development program for large format array of SIS photon detectors.

  4. Final Report Nucleic Acid System - PCR, Multiplex Assays and Sample Preparation Project

    SciTech Connect

    Koopman, R.P.; Langlois, R.G.; Nasarabadi, S.; Benett, W.J.; Richards, J.B.; Hadley, D.R.; Miles, R.R.; Brown, S.B.; Stratton, P.L.; Milanovich, F.P.

    2001-04-20

    The objective of this project was to reduce to practice the detection and identification of biological warfare pathogens by the nucleic acid recognition technique of PCR (polymerase chain reaction). This entailed not only building operationally functional instrumentation but also developing the chemical assays for detection of priority pathogens. This project had two principal deliverables: (1) design, construct, test and deliver a 24 chamber, multiplex capable suitcase sized PCR instrument, and (2) develop and reduce to practice a multiplex assay for the detection of PCR product by flow cytometry. In addition, significant resources were allocated to test and evaluation of the Hand-held Advanced Nucleic Acid Analyzer (HANAA). This project helps provide the signature and intelligence gathering community the ability to perform, on-site or remote, rapid analysis of environmental or like samples for the presence of a suite of biological warfare pathogens.

  5. Resonant cavity based time-domain multiplexing techniques for coherently combined fiber laser systems

    NASA Astrophysics Data System (ADS)

    Zhou, T.; Ruppe, J.; Stanfield, P.; Nees, J.; Wilcox, R.; Galvanauskas, A.

    2015-10-01

    This paper describes novel time-domain multiplexing techniques that use various resonant cavity configurations for increasing pulse energy extraction per each parallel amplification channel of a coherently combined array. Two different techniques are presented: a so-called N2 coherent array combining technique, applicable to a periodic pulse train, and a coherent pulse stacking amplification (CPSA) technique, applicable to a pulse burst. The first technique is a coherent combining technique, which achieves simultaneous beam combining and time-domain pulse multiplexing/down-counting using traveling-wave Fabry-Perot type resonators. The second technique is purely a time-domain pulse multiplexing technique, used with either a single amplifier or an amplifier array, which uses traveling-wave Gires-Tourmois type resonators. The importance of these techniques is that they can enable stacking of very large number of pulses, thus increasing effective amplified-pulse duration potentially by 102 to 103 times, and reducing fiber array size by the corresponding factor. This could lead to very compact coherently combined arrays even for generating very high pulse energies in the range of 1 to 100 J.

  6. Combining spatial domain multiplexing and orbital angular momentum of photon-based multiplexing to increase the bandwidth of optical fiber communication systems

    NASA Astrophysics Data System (ADS)

    Murshid, Syed; Alanzi, Saud; Hridoy, Arnob; Lovell, Gregory L.; Parhar, Gurinder; Chakravarty, Abhijit; Chowdhury, Bilas

    2016-06-01

    Spatial domain multiplexing/space division multiplexing (SDM) can increase the bandwidth of existing and futuristic optical fibers by an order of magnitude or more. In the SDM technique, we launch multiple single-mode pigtail laser sources of the same wavelength into a carrier multimode fiber at different angles. The launching angles decide the output of the carrier fiber by allocating separate spatial locations for each channel. Each channel follows a helical trajectory while traversing the length of the carrier fiber, thereby allowing spatial reuse of optical frequencies. We launch light from five different single-mode pigtail laser sources (of same wavelength) at different angles (with respect to the axis of the carrier fiber) into the carrier fiber. Owing to helical propagation, five distinct concentric donut-shaped rings with negligible crosstalk at the output end of the fiber were obtained. These SDM channels also exhibit orbital angular momentum (OAM), thereby adding an extradegree of photon freedom. We present the experimental data of five spatially multiplexed channels and compare them with simulated results to show that this technique can potentially improve the data capacity of optical fibers by an order of magnitude: A factor of five using SDM and another factor of two using OAM.

  7. An integrated readout system for drift chambers: the application of monolithic CMOS pixel sensors as segmented direct anode

    NASA Astrophysics Data System (ADS)

    Campbell, M.; Heijne, E. H. M.; Llopart, X.; Chefdeville, M.; Colas, P.; Giomataris, Y.; Colijn, A. P.; Fornaini, A.; van der Graaf, H.; Kluit, P.; Timmermans, J.; Visschers, J. L.; Schmitz, J.

    2006-01-01

    A small TPC has been read out by means of a MediPix2 readout chip as direct anode. A Micromegas foil was placed 50 μm above the chip, and electron multiplication occurred in the gap. With a He/Isobutane 80/20 mixture, gas multiplication factors up to tens of thousands were achieved, resulting in an efficiency for detecting single electrons of better than 90%. We recorded many frames containing 2D images with tracks from cosmic muons. Along these tracks, electron clusters were observed, as well as δ-rays.

  8. A nucleic acid strand displacement system for the multiplexed detection of tuberculosis-specific mRNA using quantum dots

    NASA Astrophysics Data System (ADS)

    Gliddon, H. D.; Howes, P. D.; Kaforou, M.; Levin, M.; Stevens, M. M.

    2016-05-01

    The development of rapid, robust and high performance point-of-care diagnostics relies on the advancement and combination of various areas of research. We have developed an assay for the detection of multiple mRNA molecules that combines DNA nanotechnology with fluorescent nanomaterials. The core switching mechanism is toehold-mediated strand displacement. We have used fluorescent quantum dots (QDs) as signal transducers in this assay, as they bring many benefits including bright fluorescence and multiplexing abilities. The resulting assay is capable of multiplexed detection of long RNA targets against a high concentration of background non-target RNA, with high sensitivity and specificity and limits of detection in the nanomolar range using only a standard laboratory plate reader. We demonstrate the utility of our QD-based system for the detection of two genes selected from a microarray-derived tuberculosis-specific gene expression signature. Levels of up- and downregulated gene transcripts comprising this signature can be combined to give a disease risk score, making the signature more amenable for use as a diagnostic marker. Our QD-based approach to detect these transcripts could pave the way for novel diagnostic assays for tuberculosis.The development of rapid, robust and high performance point-of-care diagnostics relies on the advancement and combination of various areas of research. We have developed an assay for the detection of multiple mRNA molecules that combines DNA nanotechnology with fluorescent nanomaterials. The core switching mechanism is toehold-mediated strand displacement. We have used fluorescent quantum dots (QDs) as signal transducers in this assay, as they bring many benefits including bright fluorescence and multiplexing abilities. The resulting assay is capable of multiplexed detection of long RNA targets against a high concentration of background non-target RNA, with high sensitivity and specificity and limits of detection in the nanomolar

  9. A kind of multilevel authentication system for multiple-image by modulated real part synthesis and iterative phase multiplexing

    NASA Astrophysics Data System (ADS)

    Pan, Xuemei; Meng, Xiangfeng; Wang, Yurong; Yang, Xiulun; Peng, Xiang; He, Wenqi; Dong, Guoyan; Chen, Hongyi

    2016-04-01

    A kind of multilevel authentication system for multiple-image based on modulated real part synthesis and iterative phase multiplexing in the Fresnel domain is proposed. In the design process of the low-level authentication system, a series of normalized real part information are iteratively generated by phase retrieval algorithm in the Fresnel domain, and the final private keys for different individual low-level certification images can be fabricated by binary amplitude modulation, superposition, synthesis, and sampling; while in the design process of the high-level authentication system, the final private keys for different individual high-level certification images can be generated by iterative phase information encoding and multiplexing. During the high-level authentication, the meaningful certification image can be reconstructed by the inverse Fresnel transform with the corresponding correct private keys, meanwhile, the correlation coefficient is utilized as judgment criterion; while in the low-level authentication, with the help of correct keys, the noise-like image with meaningless information can be recovered, but a remarkable peak output in the nonlinear correlation coefficient can be generated, which is adopted as the criterion to judge whether the low-level authentication is successful or not. Theoretical analysis and numerical simulations both verify the feasibility of the proposed method.

  10. Increment of Access Points in Integrated System of Wavelength Division Multiplexed Passive Optical Network Radio over Fiber

    PubMed Central

    Amiri, I. S.; Alavi, S. E.; Soltanian, M. R. K.; Fisal, N.; Supa’at, A. S. M.; Ahmad, H.

    2015-01-01

    This paper describes a novel technique to increase the numbers of access points (APs) in a wavelength division multiplexed-passive optical network (WDM-PON) integrated in a 100 GHz radio-over-fiber (RoF). Eight multi-carriers separated by 25 GHz intervals were generated in the range of 193.025 to 193.200 THz using a microring resonator (MRR) system incorporating an add-drop filter system. All optically generated multi-carriers were utilized in an integrated system of WDM-PON-RoF for transmission of four 43.6 Gb/sec orthogonal frequency division multiplexing (OFDM) signals. Results showed that an acceptable BER variation for different path lengths up to 25 km was achievable for all four access points and thus the transmission of four OFDM channels is feasible for a 25 km standard single mode fiber (SSMF) path length. PMID:26153536

  11. The development and application of a multiplex short tandem repeat (STR) system for identifying subspecies, individuals and sex in tigers.

    PubMed

    Zou, Zheng-Ting; Uphyrkina, Olga V; Fomenko, Pavel; Luo, Shu-Jin

    2015-07-01

    Poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. Development of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. We developed a multiplex genotyping system "tigrisPlex" to simultaneously assess 22 short tandem repeat (STR, or microsatellite) loci and a gender-identifying SRY gene, all amplified in 4 reactions using as little as 1 ng of template DNA. With DNA samples used for between-run calibration, the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles, hence making it possible to identify subspecies via bi-parentally inherited markers. We applied "tigrisPlex" to 12 confiscated specimens from Russia and identified 6 individuals (3 females and 3 males), each represented by duplicated samples and all designated as Amur tigers (Panthera tigris altaica) with high confidence. This STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products, fulfilling various conservation needs for identifying the origins of tiger samples.

  12. Genetic analysis of eight population groups living in Taiwan using a 13 X-chromosomal STR loci multiplex system.

    PubMed

    Hwa, Hsiao-Lin; Lee, James Chun-I; Chang, Yih-Yuan; Yin, Hsiang-Yi; Chen, Ya-Hui; Tseng, Li-Hui; Su, Yi-Ning; Ko, Tsang-Ming

    2011-01-01

    A 13 X-chromosomal short tandem repeat (STR) multiplex system (DXS6807, DXS8378, DSX9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7424, DXS101, GATA172D05, HPRTB, DXS8377, and DXS7423) was tested on 1,037 DNA samples from eight population groups currently living in Taiwan. Different distributions of the allelic frequencies in different populations were presented. DXS8377 and DXS101 were the two most polymorphic loci in these eight populations, whereas DXS7423 was the least informative marker in most of the populations studied. The genetic distances between the populations and the constructed phylogenetic tree revealed a long genetic distance between Asian and Caucasian populations as well as isolation of the Tao population. The phylogenetic tree grouped populations into clusters compatible with their ethnogeographic relationships. This 13 X-chromosomal short tandem repeat multiplex system offers a considerable number of polymorphic patterns in different populations. This system can be useful in forensic identification casework and ethnogeographic research.

  13. The development and application of a multiplex short tandem repeat (STR) system for identifying subspecies, individuals and sex in tigers.

    PubMed

    Zou, Zheng-Ting; Uphyrkina, Olga V; Fomenko, Pavel; Luo, Shu-Jin

    2015-07-01

    Poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. Development of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. We developed a multiplex genotyping system "tigrisPlex" to simultaneously assess 22 short tandem repeat (STR, or microsatellite) loci and a gender-identifying SRY gene, all amplified in 4 reactions using as little as 1 ng of template DNA. With DNA samples used for between-run calibration, the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles, hence making it possible to identify subspecies via bi-parentally inherited markers. We applied "tigrisPlex" to 12 confiscated specimens from Russia and identified 6 individuals (3 females and 3 males), each represented by duplicated samples and all designated as Amur tigers (Panthera tigris altaica) with high confidence. This STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products, fulfilling various conservation needs for identifying the origins of tiger samples. PMID:25950598

  14. High-throughput in vivo genotoxicity testing: an automated readout system for the somatic mutation and recombination test (SMART).

    PubMed

    Lombardot, Benoit; Oh, Chun-Taek; Kwak, Jihoon; Genovesio, Auguste; Kang, Myungjoo; Hansen, Michael Adsett Edberg; Han, Sung-Jun

    2015-01-01

    Genotoxicity testing is an important component of toxicity assessment. As illustrated by the European registration, evaluation, authorization, and restriction of chemicals (REACH) directive, it concerns all the chemicals used in industry. The commonly used in vivo mammalian tests appear to be ill adapted to tackle the large compound sets involved, due to throughput, cost, and ethical issues. The somatic mutation and recombination test (SMART) represents a more scalable alternative, since it uses Drosophila, which develops faster and requires less infrastructure. Despite these advantages, the manual scoring of the hairs on Drosophila wings required for the SMART limits its usage. To overcome this limitation, we have developed an automated SMART readout. It consists of automated imaging, followed by an image analysis pipeline that measures individual wing genotoxicity scores. Finally, we have developed a wing score-based dose-dependency approach that can provide genotoxicity profiles. We have validated our method using 6 compounds, obtaining profiles almost identical to those obtained from manual measures, even for low-genotoxicity compounds such as urethane. The automated SMART, with its faster and more reliable readout, fulfills the need for a high-throughput in vivo test. The flexible imaging strategy we describe and the analysis tools we provide should facilitate the optimization and dissemination of our methods.

  15. High-Throughput In Vivo Genotoxicity Testing: An Automated Readout System for the Somatic Mutation and Recombination Test (SMART)

    PubMed Central

    Kwak, Jihoon; Genovesio, Auguste; Kang, Myungjoo; Hansen, Michael Adsett Edberg; Han, Sung-Jun

    2015-01-01

    Genotoxicity testing is an important component of toxicity assessment. As illustrated by the European registration, evaluation, authorization, and restriction of chemicals (REACH) directive, it concerns all the chemicals used in industry. The commonly used in vivo mammalian tests appear to be ill adapted to tackle the large compound sets involved, due to throughput, cost, and ethical issues. The somatic mutation and recombination test (SMART) represents a more scalable alternative, since it uses Drosophila, which develops faster and requires less infrastructure. Despite these advantages, the manual scoring of the hairs on Drosophila wings required for the SMART limits its usage. To overcome this limitation, we have developed an automated SMART readout. It consists of automated imaging, followed by an image analysis pipeline that measures individual wing genotoxicity scores. Finally, we have developed a wing score-based dose-dependency approach that can provide genotoxicity profiles. We have validated our method using 6 compounds, obtaining profiles almost identical to those obtained from manual measures, even for low-genotoxicity compounds such as urethane. The automated SMART, with its faster and more reliable readout, fulfills the need for a high-throughput in vivo test. The flexible imaging strategy we describe and the analysis tools we provide should facilitate the optimization and dissemination of our methods. PMID:25830368

  16. Developed and evaluated a multiplex mRNA profiling system for body fluid identification in Chinese Han population.

    PubMed

    Song, Feng; Luo, Haibo; Hou, Yiping

    2015-10-01

    In forensic casework, identification the cellular origin from a biological sample is crucial to the case investigation and reconstruction in crime scene. DNA/RNA co-extraction for STR typing and human body fluids identification has been proposed as an efficient and comprehensive assay for forensic analysis. Several cell-specific messenger RNA (mRNA) markers for identification of the body fluids have been proposed by previous studies. In this study, a novel multiplex mRNA profiling system included 19 markers was developed and performed by reverse transcription endpoint polymerase chain reaction (RT-PCR). The multiplex combined 3 housekeeping gene markers and 16 cell-specific markers that have been used to identify five types of human body fluids: peripheral blood, semen, saliva, vaginal secretions and menstrual blood. The specificity, sensitivity, stability and detectability of the mixture were explored in our study. Majority of the cell-specific mRNA markers showed high specificity, although cross-reactivity was observed sporadically. Specific profiling for per body fluid was obtained. Moreover, the interpretation guidelines for inference of body fluid types were performed according to the A. Lindenbergh et al. The scoring guidelines can be applied to any RNA multiplex, which was based on six different scoring categories (observed, observed and fits, sporadically observed and fits, not observed, sporadically observed, not reliable, and non-specific due to high input). The simultaneous extraction of DNA showed positive full or partial profiling results of all samples. It demonstrated that the approach of combined STR-profiling and RNA profiling was suitable and reliable to detect the donor and origin of human body fluids in Chinese Han population. PMID:26311108

  17. Developed and evaluated a multiplex mRNA profiling system for body fluid identification in Chinese Han population.

    PubMed

    Song, Feng; Luo, Haibo; Hou, Yiping

    2015-10-01

    In forensic casework, identification the cellular origin from a biological sample is crucial to the case investigation and reconstruction in crime scene. DNA/RNA co-extraction for STR typing and human body fluids identification has been proposed as an efficient and comprehensive assay for forensic analysis. Several cell-specific messenger RNA (mRNA) markers for identification of the body fluids have been proposed by previous studies. In this study, a novel multiplex mRNA profiling system included 19 markers was developed and performed by reverse transcription endpoint polymerase chain reaction (RT-PCR). The multiplex combined 3 housekeeping gene markers and 16 cell-specific markers that have been used to identify five types of human body fluids: peripheral blood, semen, saliva, vaginal secretions and menstrual blood. The specificity, sensitivity, stability and detectability of the mixture were explored in our study. Majority of the cell-specific mRNA markers showed high specificity, although cross-reactivity was observed sporadically. Specific profiling for per body fluid was obtained. Moreover, the interpretation guidelines for inference of body fluid types were performed according to the A. Lindenbergh et al. The scoring guidelines can be applied to any RNA multiplex, which was based on six different scoring categories (observed, observed and fits, sporadically observed and fits, not observed, sporadically observed, not reliable, and non-specific due to high input). The simultaneous extraction of DNA showed positive full or partial profiling results of all samples. It demonstrated that the approach of combined STR-profiling and RNA profiling was suitable and reliable to detect the donor and origin of human body fluids in Chinese Han population.

  18. Novel Multiplexing Technique for Detector and Mixer Arrays

    NASA Technical Reports Server (NTRS)

    Karasik, Boris S.; McGrath, William R.

    2001-01-01

    Future submillimeter and far-infrared space telescopes will require large-format (many 1000's of elements) imaging detector arrays to perform state-of-the-art astronomical observations. A crucial issue related to a focal plane array is a readout scheme which is compatible with large numbers of cryogenically-cooled (typically < 1 K) detectors elements. When the number of elements becomes of the order of thousands, the physical layout for individual readout amplifiers becomes nearly impossible to realize for practical systems. Another important concern is the large number of wires leading to a 0.1-0.3 K platform. In the case of superconducting transition edge sensors (TES), a scheme for time-division multiplexing of SQUID read-out amplifiers has been recently demonstrated. In this scheme the number of SQUIDs is equal to the number (N) of the detectors, but only one SQUID is turned on at a time. The SQUIDs are connected in series in each column of the array, so the number of wires leading to the amplifiers can be reduced, but it is still of the order of N. Another approach uses a frequency domain multiplexing scheme of the bolometer array. The bolometers are biased with ac currents whose frequencies are individual for each element and are much higher than the bolometer bandwidth. The output signals are connected in series in a summing loop which is coupled to a single SQUID amplifier. The total number of channels depends on the ratio between the SQUID bandwidth and the bolometer bandwidth and can be at least 100 according to the authors. An important concern about this technique is a contribution of the out-of-band Johnson noise which multiplies by factor N(exp 1/2) for each frequency channel. We propose a novel solution for large format arrays based on the Hadamard transform coding technique which requires only one amplifier to read out the entire array of potentially many 1000's of elements and uses approximately 10 wires between the cold stage and room temperature

  19. Development of a 24-locus multiplex system to incorporate the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS).

    PubMed

    Guo, Fei; Shen, Hongying; Tian, Huaizhou; Jin, Ping; Jiang, Xianhua

    2014-01-01

    The 24-locus multiplex system allows co-amplification and fluorescent detection of 24 loci (23 STR loci and Amelogenin), including STR loci in the Combined DNA Index System (CODIS) and the ESS (European Standard Set) as well as five additional loci (D2S1338, D6S1043, D19S433, Penta D and Penta E) commonly used in commercial kits. It facilitates data sharing and minimizes adventitious matches within national or between international DNA databases. Additionally, the system can amplify directly from blood and buccal samples spotted on filter paper and swabs and reduce the cycling time to less than one hour and a half. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house with a design strategy to work in this multiplex. Developmental validation experiments followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815-2009) guidelines. The system was evaluated by species specificity, sensitivity, stability, precision and accuracy, case-type samples, population, mixture and PCR-based studies. The results demonstrate that the 24-locus multiplex system is a robust and reliable identification assay as required for forensic DNA typing and databasing.

  20. Low-cost, multiplexed biosensor for disease diagnosis

    NASA Astrophysics Data System (ADS)

    Myatt, Christopher J.; Delaney, Marie; Todorof, Kathryn; Heil, James; Givens, Monique; Schooley, Robert T.; Lochhead, Michael J.

    2009-02-01

    Cost-effective disease diagnosis in resource-limited settings remains a critical global health challenge. Qualitative rapid tests based on lateral flow technology provide valuable screening information, but require relatively expensive confirmatory tests and generally lack quantitation. We report on a fluorescence technology that combines low cost instrumented readout with passive pumping in a disposable cartridge. The detection system utilizes a novel waveguide illumination approach in conjunction with commercial CMOS imagers. Total instrument cost in production are projected to be around $100 This cost structure and instrument ease of use will enable use in point-of-care settings, outside of centralized laboratories. The system has been used for detection and analysis of proteins, antibodies, nucleic acids, and cells. Here we will report first on our development of a multiplexed, array-based serology assay for HIV and common AIDS co-infections. Data will be presented for HIV/HCV antibody testing in human serum samples. In addition, we will present data on the use of the system for sensitive detection of bacterial RNA. Current detection limit for the model multiplexed RNA sandwich assay is 1 femtomolar target RNA. Finally, a high magnification version of the system is used to image immunostained human T cells.

  1. Field-based multiplex and quantitative assay platforms for diagnostics

    NASA Astrophysics Data System (ADS)

    Venkatasubbarao, Srivatsa; Dixon, C. Edward; Chipman, Russell; Scherer, Axel; Beshay, Manal; Kempen, Lothar U.; Chandra Sekhar, Jai Ganesh; Yan, Hong; Puccio, Ava; Okonkwo, David; McClain, Stephen; Gilbert, Noah; Vyawahare, Saurabh

    2011-06-01

    The U.S. military has a continued interest in the development of handheld, field-usable sensors and test kits for a variety of diagnostic applications, such as traumatic brain injury (TBI) and infectious diseases. Field-use presents unique challenges for biosensor design, both for the readout unit and for the biological assay platform. We have developed robust biosensor devices that offer ultra-high sensitivity and also meet field-use needs. The systems under development include a multiplexed quantitative lateral flow test strip for TBI diagnostics, a field test kit for the diagnosis of pathogens endemic to the Middle East, and a microfluidic assay platform with a label-free reader for performing complex biological automated assays in the field.

  2. Spectral contents readout of birefringent sensor

    NASA Technical Reports Server (NTRS)

    Redner, Alex S.

    1989-01-01

    The technical objective of this research program was to develop a birefringent sensor, capable of measuring strain/stress up to 2000 F and a readout system based on Spectral Contents analysis. As a result of the research work, a data acquisition system was developed, capable of measuring strain birefringence in a sensor at 2000 F, with multi-point static and dynamic capabilities. The system uses a dedicated spectral analyzer for evaluation of stress-birefringence and a PC-based readout. Several sensor methods were evaluated. Fused silica was found most satisfactory. In the final evaluation, measurements were performed up to 2000 F and the system performance exceeded expectations.

  3. Practical angular-multiplexing holographic data storage system with 2 terabyte capacity and 1 gigabit transfer rate

    NASA Astrophysics Data System (ADS)

    Hoshizawa, Taku; Shimada, Ken-ichi; Fujita, Kouji; Tada, Yukinobu

    2016-09-01

    Aiming at the realization of an optical data storage system with a large disc capacity and a high transfer rate suitable for digital data archiving, we have defined the specifications of an angular-multiplexing holographic data storage system with a disc capacity of 2 TB and a transfer rate of 1 Gbps on the basis of a run-length-limited (RLL) high-density recording method using RLL modulation. To realize the specifications, RLL turbo coding, a servo system for the reference beam angle, and a servo system for book tracking were newly invented. Also, to satisfy the specifications, a holographic disc and several key components were developed in cooperation with several specialty companies. Eventually, we confirmed a high-density recording of 2.4 Tbit/in.2 achieved by the RLL high-density recording method, using evaluation equipment complying with the specifications for single book recording.

  4. Multiplexed encryption using chaotic systems with multiple stochastic-delayed feedbacks.

    PubMed

    Rontani, D; Sciamanna, M; Locquet, A; Citrin, D S

    2009-12-01

    We propose an efficient and fast bit-multiplexed encryption scheme exploiting hyperchaotic regimes of a single nonlinear oscillator with multiple time-delay feedback loops. Each data stream is encrypted by digitally modulating the values of the various time delays and decrypted using chaos synchronization and cross-correlation measurements. We have numerically applied our approach to an optoelectronic chaotic oscillator based on standard semiconductor lasers subjected to multiple feedbacks and have demonstrated successful data transmission and recovery between multiple users at several Gbits/s on a single communication channel.

  5. Joint mitigation of laser phase noise and fiber nonlinearity for polarization-multiplexed QPSK and 16-QAM coherent transmission systems.

    PubMed

    Morsy-Osman, Mohamed; Zhuge, Qunbi; Chen, Lawrence R; Plant, David V

    2011-12-12

    We propose the use of pilot-aided (PA) transmission, enabled by single-sideband-subcarrier modulation of both quadratures in the DSP-domain, in single-carrier systems to mitigate jointly laser phase noise and fiber nonlinearity. In addition to tolerance against laser phase noise, we show that the proposed scheme also improves the nonlinear tolerance of both polarization-division-multiplexed (PDM) QPSK and 16-QAM coherent transmission systems by increasing the maximum allowable launch power by 1 dB and 1.5 dB, respectively. The improved nonlinear performance of both systems also manifests itself as an increase in the maximum reach by 720 km and 480 km, respectively. Finally, when digital-to-analog converters (DACs) with lower bit resolutions are used at the transmitter, PA transmission is shown to preserve the same performance improvement over the non-PA case.

  6. Multiplexed Holographic Data Storage in Bacteriorhodopsin

    NASA Technical Reports Server (NTRS)

    Mehrl, David J.; Krile, Thomas F.

    1999-01-01

    Biochrome photosensitive films in particular Bacteriorhodopsin exhibit features which make these materials an attractive recording medium for optical data storage and processing. Bacteriorhodopsin films find numerous applications in a wide range of optical data processing applications; however the short-term memory characteristics of BR limits their applications for holographic data storage. The life-time of the BR can be extended using cryogenic temperatures [1], although this method makes the system overly complicated and unstable. Longer life-times can be provided in one modification of BR - the "blue" membrane BR [2], however currently available films are characterized by both low diffraction efficiency and difficulties in providing photoreversible recording. In addition, as a dynamic recording material, the BR requires different wavelengths for recording and reconstructing of optical data in order to prevent the information erasure during its readout. This fact also put constraints on a BR-based Optical Memory, due to information loss in holographic memory systems employing the two-lambda technique for reading-writing thick multiplexed holograms.

  7. Read-out electronics for DC squid magnetic measurements

    DOEpatents

    Ganther, Jr., Kenneth R.; Snapp, Lowell D.

    2002-01-01

    Read-out electronics for DC SQUID sensor systems, the read-out electronics incorporating low Johnson noise radio-frequency flux-locked loop circuitry and digital signal processing algorithms in order to improve upon the prior art by a factor of at least ten, thereby alleviating problems caused by magnetic interference when operating DC SQUID sensor systems in magnetically unshielded environments.

  8. Nanosecond monolithic CMOS readout cell

    DOEpatents

    Souchkov, Vitali V.

    2004-08-24

    A pulse shaper is implemented in monolithic CMOS with a delay unit formed of a unity gain buffer. The shaper is formed of a difference amplifier having one input connected directly to an input signal and a second input connected to a delayed input signal through the buffer. An elementary cell is based on the pulse shaper and a timing circuit which gates the output of an integrator connected to the pulse shaper output. A detector readout system is formed of a plurality of elementary cells, each connected to a pixel of a pixel array, or to a microstrip of a plurality of microstrips, or to a detector segment.

  9. Electrical delay line multiplexing for pulsed mode radiation detectors

    NASA Astrophysics Data System (ADS)

    Vinke, Ruud; Yeom, Jung Yeol; Levin, Craig S.

    2015-04-01

    Medical imaging systems are composed of a large number of position sensitive radiation detectors to provide high resolution imaging. For example, whole-body Positron Emission Tomography (PET) systems are typically composed of thousands of scintillation crystal elements, which are coupled to photosensors. Thus, PET systems greatly benefit from methods to reduce the number of data acquisition channels, in order to reduce the system development cost and complexity. In this paper we present an electrical delay line multiplexing scheme that can significantly reduce the number of readout channels, while preserving the signal integrity required for good time resolution performance. We experimented with two 4 × 4 LYSO crystal arrays, with crystal elements having 3 mm × 3 mm × 5 mm and 3 mm × 3 mm × 20 mm dimensions, coupled to 16 Hamamatsu MPPC S10931-050P SiPM elements. Results show that each crystal could be accurately identified, even in the presence of scintillation light sharing and inter-crystal Compton scatter among neighboring crystal elements. The multiplexing configuration degraded the coincidence timing resolution from ∼243 ps FWHM to ∼272 ps FWHM when 16 SiPM signals were combined into a single channel for the 4 × 4 LYSO crystal array with 3 mm × 3 mm × 20 mm crystal element dimensions, in coincidence with a 3 mm × 3 mm × 5 mm LYSO crystal pixel. The method is flexible to allow multiplexing configurations across different block detectors, and is scalable to an entire ring of detectors.

  10. De-Li-DAQ-2D - a new data acquisition system for position-sensitive neutron detectors with delay-line readout

    NASA Astrophysics Data System (ADS)

    Levchanovskiy, F. V.; Murashkevich, S. M.

    2016-09-01

    Software for a data acquisition system of modern one- and two-dimensional position-sensitive detectors with delay-line readout, which includes a software interface to a new electronic module De-Li-DAQ-2D with a USB interface, is presented. The new system after successful tests on the stand and on several spectrometers of the IBR-2 reactor has been integrated into the software complex SONIX+ [1]. The De-Li- DAQ-2D module [2] contains an 8-channel time-code converter (TDC-GPX) with a time resolution of 80 ps, field programmable gate array (FPGA), 1 Gbyte histogram memory and high-speed interface with a fiber-optic communication line. A real count rate is no less than 106 events/s. The De-Li-DAQ-2D module is implemented in the NIM standard. The De-Li-DAQ-2D module can operate in two modes: histogram mode and list mode.

  11. Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample.

    PubMed

    Robino, C; Giolitti, A; Gino, S; Torre, C

    2006-09-01

    Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man. PMID:16862443

  12. Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample.

    PubMed

    Robino, C; Giolitti, A; Gino, S; Torre, C

    2006-09-01

    Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

  13. D-Zero muon readout electronics design

    SciTech Connect

    Baldin, B.; Hansen, S.; Los, S.; Matveev, M.; Vaniev, V.

    1996-11-01

    The readout electronics designed for the D{null} Muon Upgrade are described. These electronics serve three detector subsystems and one trigger system. The front-ends and readout hardware are synchronized by means of timing signals broadcast from the D{null} Trigger Framework. The front-end electronics have continuously running digitizers and two levels of buffering resulting in nearly deadtimeless operation. The raw data is corrected and formatted by 16- bit fixed point DSP processors. These processors also perform control of the data buffering. The data transfer from the front-end electronics located on the detector platform is performed by serial links running at 160 Mbit/s. The design and test results of the subsystem readout electronics and system interface are discussed.

  14. Development of a multiplex system to assess DNA persistence in taphonomic studies.

    PubMed

    Nazir, Muhammad S; Iyavoo, Sasitaran; Alimat, Sharizah; Zahra, Nathalie; Sanqoor, Sheikha H; Smith, Judith A; Moffatt, Colin; Goodwin, Will

    2013-12-01

    In this study, we have developed a PCR multiplex that can be used to assess DNA degradation and at the same time monitor for inhibition: primers have been designed to amplify human, pig, and rabbit DNA, allowing pig and rabbit to be used as experimental models for taphonomic research, but also enabling studies on human DNA persistence in forensic evidence. Internal amplified controls have been added to monitor for inhibition, allowing the effects of degradation and inhibition to be differentiated. Sequence data for single-copy nuclear recombination activation gene (RAG-1) from human, pig, and rabbit were aligned to identify conserved regions and primers were designed that targeted amplicons of 70, 194, 305, and 384 bp. Robust amplification in all three species was possible using as little as 0.3 ng of template DNA. These have been combined with primers that will amplify a bacterial DNA template within the PCR. The multiplex has been evaluated in a series of experiments to gain more knowledge of DNA persistence in soft tissues, which can be important when assessing what material to collect following events such as mass disasters or conflict, when muscle or bone material can be used to aid with the identification of human remains. The experiments used pigs as a model species. When whole pig bodies were exposed to the environment in Northwest England, DNA in muscle tissue persisted for over 24 days in the summer and over 77 days in the winter, with full profiles generated from these samples. In addition to time, accumulated degree days (ADD) were also used as a measure that combines both time and temperature-24 days was in summer equivalent to 295 ADD whereas 77 days in winter was equivalent to 494 ADD.

  15. NASA Communications Division (NASCOM) Tracking and Data Relay Satellite System (TDRSS) shuttle multiplexer-demultiplexer data system (MDM) and supporting items

    NASA Technical Reports Server (NTRS)

    New, S. R.

    1981-01-01

    The multiplexer-demultiplexer (MDM) project included the design, documentation, manufacture, and testing of three MDM Data Systems. The equipment is contained in 59 racks, and includes more than 3,000 circuit boards and 600 microprocessors. Spares, circuit card testers, a master set of programmable integrated circuits, and a program development system were included as deliverables. All three MDM's were installed, and were operationally tested. The systems performed well with no major problems. The progress and problems analysis, addresses schedule conformance, new technology, items awaiting government approval, and project conclusions are summarized. All contract modifications are described.

  16. NASA Communications Division (NASCOM) Tracking and Data Relay Satellite System (TDRSS) shuttle multiplexer-demultiplexer data system (MDM) and supporting items

    NASA Astrophysics Data System (ADS)

    New, S. R.

    1981-06-01

    The multiplexer-demultiplexer (MDM) project included the design, documentation, manufacture, and testing of three MDM Data Systems. The equipment is contained in 59 racks, and includes more than 3,000 circuit boards and 600 microprocessors. Spares, circuit card testers, a master set of programmable integrated circuits, and a program development system were included as deliverables. All three MDM's were installed, and were operationally tested. The systems performed well with no major problems. The progress and problems analysis, addresses schedule conformance, new technology, items awaiting government approval, and project conclusions are summarized. All contract modifications are described.

  17. Discrete wavelength selection for the optical readout of a metamaterial biosensing system for glucose concentration estimation via a support vector regression model.

    PubMed

    Teutsch, T; Mesch, M; Giessen, H; Tarin, C

    2015-01-01

    In this contribution, a method to select discrete wavelengths that allow an accurate estimation of the glucose concentration in a biosensing system based on metamaterials is presented. The sensing concept is adapted to the particular application of ophthalmic glucose sensing by covering the metamaterial with a glucose-sensitive hydrogel and the sensor readout is performed optically. Due to the fact that in a mobile context a spectrometer is not suitable, few discrete wavelengths must be selected to estimate the glucose concentration. The developed selection methods are based on nonlinear support vector regression (SVR) models. Two selection methods are compared and it is shown that wavelengths selected by a sequential forward feature selection algorithm achieves an estimation improvement. The presented method can be easily applied to different metamaterial layouts and hydrogel configurations.

  18. Low-power priority Address-Encoder and Reset-Decoder data-driven readout for Monolithic Active Pixel Sensors for tracker system

    NASA Astrophysics Data System (ADS)

    Yang, P.; Aglieri, G.; Cavicchioli, C.; Chalmet, P. L.; Chanlek, N.; Collu, A.; Gao, C.; Hillemanns, H.; Junique, A.; Kofarago, M.; Keil, M.; Kugathasan, T.; Kim, D.; Kim, J.; Lattuca, A.; Marin Tobon, C. A.; Marras, D.; Mager, M.; Martinengo, P.; Mazza, G.; Mugnier, H.; Musa, L.; Puggioni, C.; Rousset, J.; Reidt, F.; Riedler, P.; Snoeys, W.; Siddhanta, S.; Usai, G.; van Hoorne, J. W.; Yi, J.

    2015-06-01

    Active Pixel Sensors used in High Energy Particle Physics require low power consumption to reduce the detector material budget, low integration time to reduce the possibilities of pile-up and fast readout to improve the detector data capability. To satisfy these requirements, a novel Address-Encoder and Reset-Decoder (AERD) asynchronous circuit for a fast readout of a pixel matrix has been developed. The AERD data-driven readout architecture operates the address encoding and reset decoding based on an arbitration tree, and allows us to readout only the hit pixels. Compared to the traditional readout structure of the rolling shutter scheme in Monolithic Active Pixel Sensors (MAPS), AERD can achieve a low readout time and a low power consumption especially for low hit occupancies. The readout is controlled at the chip periphery with a signal synchronous with the clock, allows a good digital and analogue signal separation in the matrix and a reduction of the power consumption. The AERD circuit has been implemented in the TowerJazz 180 nm CMOS Imaging Sensor (CIS) process with full complementary CMOS logic in the pixel. It works at 10 MHz with a matrix height of 15 mm. The energy consumed to read out one pixel is around 72 pJ. A scheme to boost the readout speed to 40 MHz is also discussed. The sensor chip equipped with AERD has been produced and characterised. Test results including electrical beam measurement are presented.

  19. Multifunction optical filter with a Michelson-Gires-Tournois interferometer for wavelength-division-multiplexed network system applications.

    PubMed

    Dingel, B B; Izutsu, M

    1998-07-15

    We propose using a novel multifunction optical filter with a Michelson-Gires-Tournois interferometer (MGTI) for future smart wavelength-division-multiplexed network system applications. The MGTI filter is a typical Michelson interferometer in which one of its reflecting mirrors is replaced with a Gires-Tournois resonator. One unique feature of this device is that it can function as a channel-passing (CP), a channel-dropping (CD), or a wide-bandpass (BP) filter, depending on the interferometer arm-length difference. Other interesting features are that (1) the linewidths of both the CP and the CD filter are twice as narrow as that of a typical Fabry-Perot filter with similar parameters, (2) theoretical visibility is always unity regardless of the mirror reflectance value, and (3) the BP filter has an excellent boxlike response function. Numerical results showing these characteristics are presented. PMID:18087440

  20. Survivable wavelength-division multiplexing passive optical network system with centralized protection routing scheme and efficient wavelength utilization

    NASA Astrophysics Data System (ADS)

    Zhou, Zhao; Xiao, Shilin; Bi, Meihua; Qi, Tao; Li, Pingqing; Hu, Weisheng

    2013-09-01

    A survivable wavelength-division multiplexing passive optical network (WDM-PON) with centralized protection scheme and colorless optical network units is proposed. The sideband channels generated via optical carrier suppression technique are used to carry both the downstream (DS) and the upstream (US) remodulated signals for two neighboring sub-PONs. By employing alternate path routing of sideband channels, the centralized protection scheme can provide reliable protection against any failure in feed fibers, remote node, and distribution fibers. The number of extra fibers is greatly reduced, and efficient utilization of wavelength resource is achieved. The feasibility of the proposed system is verified by 10-Gb/s DS and 1.25-Gb/s US signals over 25-km single-mode fiber transmission in both working and protection modes.

  1. The multianalyser system of the three axes neutron spectrometer PUMA: Pilot experiments with the innovative multiplex technique

    NASA Astrophysics Data System (ADS)

    Sobolev, Oleg; Hoffmann, Ron; Gibhardt, Holger; Jünke, Norbert; Knorr, Andreas; Meyer, Volker; Eckold, Götz

    2015-02-01

    A new type of multiplex technique for three axes neutron spectrometers has been realized and successfully commissioned at the PUMA spectrometer at FRM II. Consisting of eleven analyser-detector channels which can be configured individually, this technique is especially suitable for kinetic experiments where a single excitation spectrum is recorded as a function of time without the need to move the spectrometer. On a time-scale of seconds an entire spectrum can be recorded thus allowing users to monitor changes during fast kinetic processes in single shot experiments without the need for stroboscopic techniques. Moreover, the multianalyser system provides an efficient and rapid tool for mapping excitations in (Q,ω)-space. The results of pilot experiments demonstrate the performance of this new technique and a user-friendly software is presented which assists users during their experiments.

  2. The data acquisition system for a fixed target experiment at NICA complex at JINR and its connection to the ATLAS TileCal readout electronics

    NASA Astrophysics Data System (ADS)

    Tomiwa, K. G.; Slepnev, I.; Bazylev, S.

    2015-10-01

    Today's large-scale science projects have always encountered challenges in processing large data flow from the experiments, the ATLAS detector records proton-proton collisions provided by the Large Hadron Collider (LHC) at CERN every 50 ns which results in a total data flow of 10 Pb/s. These data must be reduced to the science data product for further analysis, thus a very fast decisions need to be executed, to modify this large amounts of data at high rates. The capabilities required to support this scale of data movement is development and improvement of high-throughput electronics. The upgraded LHC will provide collisions at rates that will be at least 10 times higher than those of today due to it's luminosity by 2022. This will require a complete redesign of the read-out electronics and Processing Units (PU) in the Tile-calorimeter (TileCal) of the ATLAS experiment. A general purpose, high-throughput PU has been developed for the TileCal at CERN, by using several ARM-processors in cluster configuration. The PU is capable of handling large data throughput and apply advanced operations at high rates. This system has been proposed for the fixed target experiment at NICA complex to handle the first level processes and event building. The aim of this work is to have a look at the architecture of the data acquisition system (DAQ) of the fixed target experiment at the NICA complex at JINR, by compiling the data-flow requirements of all the subcomponents. Furthermore, the VME DAQ modules characteristics to control, triggering and data acquisition will be described in order to define the DAQ with maximum readout efficiency, no dead time and data selection and compression.

  3. Multiplexed Enrichment and Detection of Malarial Biomarkers Using a Stimuli-Responsive Iron Oxide and Gold Nanoparticle Reagent System

    PubMed Central

    Nash, Michael A.; Waitumbi, John N.; Hoffman, Allan S.; Yager, Paul; Stayton, Patrick S.

    2014-01-01

    There is a need for simple yet robust biomarker and antigen purification and enrichment strategies that are compatible with current rapid diagnostic modalities. Here, a stimuli-responsive nanoparticle system is presented for multiplexed magneto-enrichment and non-instrumented lateral flow strip detection of model antigens from spiked pooled plasma. The integrated reagent system allows purification and enrichment of the gold-labeled biomarker half-sandwich that can be applied directly to lateral flow test strips. A linear diblock copolymer with a thermally-responsive poly(N-isopropylacrylamide) (pNIPAm) segment and a gold-binding block composed of NIPAm-co-N,N-dimethylaminoethylacrylamide (DMAEAm) was prepared by reversible addition fragmentation chain transfer (RAFT) polymerization. The diblock copolymer was used to functionalize gold nanoparticles (AuNPs), with subsequent bioconjugation to yield thermally-responsive pNIPAm-AuNPs that were co-decorated with streptavidin. These AuNPs efficiently complexed biotinylated capture antibody reagents that were bound to picomolar quantities of pan-aldolase and Plasmodium Falciparum histidine rich protein 2 (PfHRP2) in spiked pooled plasma samples. The gold-labeled biomarker half-sandwich was then purified and enriched using 10 nm thermally-responsive magnetic nanoparticles that were similarly decorated with pNIPAm. When a thermal stimulus was applied in conjunction with a magnetic field, co-aggregation of the AuNP-half-sandwiches with the pNIPAm-coated iron oxide nanoparticles created large aggregates that were efficiently magnetophoresed and separated from bulk serum. The purified biomarkers from a spiked pooled plasma sample could be concentrated 50-fold into a small volume and applied directly to a commercial multiplexed lateral flow strip to dramatically improve the signal-to-noise ratio and test sensitivity. PMID:22804625

  4. Use of a Hierarchical Oligonucleotide Primer Extension Approach for Multiplexed Relative Abundance Analysis of Methanogens in Anaerobic Digestion Systems

    PubMed Central

    Chuang, Hui-Ping; Hsu, Mao-Hsuan; Chen, Wei-Yu

    2013-01-01

    In this study, we established a rapid multiplex method to detect the relative abundances of amplified 16S rRNA genes from known cultivatable methanogens at hierarchical specificities in anaerobic digestion systems treating industrial wastewater and sewage sludge. The method was based on the hierarchical oligonucleotide primer extension (HOPE) technique and combined with a set of 27 primers designed to target the total archaeal populations and methanogens from 22 genera within 4 taxonomic orders. After optimization for their specificities and detection sensitivity under the conditions of multiple single-nucleotide primer extension reactions, the HOPE approach was applied to analyze the methanogens in 19 consortium samples from 7 anaerobic treatment systems (i.e., 513 reactions). Among the samples, the methanogen populations detected with order-level primers accounted for >77.2% of the PCR-amplified 16S rRNA genes detected using an Archaea-specific primer. The archaeal communities typically consisted of 2 to 7 known methanogen genera within the Methanobacteriales, Methanomicrobiales, and Methanosarcinales and displayed population dynamic and spatial distributions in anaerobic reactor operations. Principal component analysis of the HOPE data further showed that the methanogen communities could be clustered into 3 distinctive groups, in accordance with the distribution of the Methanosaeta, Methanolinea, and Methanomethylovorans, respectively. This finding suggested that in addition to acetotrophic and hydrogenotrophic methanogens, the methylotrophic methanogens might play a key role in the anaerobic treatment of industrial wastewater. Overall, the results demonstrated that the HOPE approach is a specific, rapid, and multiplexing platform to determine the relative abundances of targeted methanogens in PCR-amplified 16S rRNA gene products. PMID:24077716

  5. Reducing multiplexing artifacts in multi-pinhole SPECT with a stacked silicon-germanium system: a simulation study.

    PubMed

    Johnson, Lindsay C; Shokouhi, Sepideh; Peterson, Todd E

    2014-12-01

    In pinhole single photon emission computed tomography (SPECT), multi-pinhole collimators can increase sensitivity but may lead to projection overlap, or multiplexing, which can cause image artifacts. In this work, we explore whether a stacked-detector configuration with a germanium and a silicon detector, used with 123I (27-32, 159 keV), where little multiplexing occurs in the Si projections, can reduce image artifacts caused by highly-multiplexed Ge projections. Simulations are first used to determine a reconstruction method that combines the Si and Ge projections to maximize image quality. Next, simulations of different pinhole configurations (varying projection multiplexing) in conjunction with digital phantoms are used to examine whether additional Si projections mitigate artifacts from the multiplexing in the Ge projections. Reconstructed images using both Si and Ge data are compared to those using Ge data alone. Normalized mean-square error and normalized standard deviation provide a quantitative evaluation of reconstructed images' error and noise, respectively, and are used to evaluate the impact of the additional nonmultiplexed data on image quality. For a qualitative comparison, the differential point response function is used to examine multiplexing artifacts. Results show that in cases of highly-multiplexed Ge projections, the addition of low-multiplexed Si projections helps to reduce image artifacts both quantitatively and qualitatively.

  6. Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.

    PubMed

    Rocchetti, Talita T; Silbert, Suzane; Gostnell, Alicia; Kubasek, Carly; Widen, Raymond

    2016-06-01

    A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.

  7. A multiplex (m)RNA-profiling system for the forensic identification of body fluids and contact traces.

    PubMed

    Lindenbergh, Alexander; de Pagter, Mirjam; Ramdayal, Geeta; Visser, Mijke; Zubakov, Dmitry; Kayser, Manfred; Sijen, Titia

    2012-09-01

    In current forensic practice, information about the possible biological origin of forensic traces is mostly determined using protein-based presumptive testing. Recently, messenger RNA-profiling has emerged as an alternative strategy to examine the biological origin. Here we describe the development of a single multiplex mRNA-based system for the discrimination of the most common forensic body fluids as well as skin cells. A DNA/RNA co-isolation protocol was established that results in DNA yields equivalent to our standard in-house validated DNA extraction procedure which uses silica-based columns. An endpoint RT-PCR assay was developed that simultaneously amplifies 19 (m)RNA markers. This multiplex assay analyses three housekeeping, three blood, two saliva, two semen, two menstrual secretion, two vaginal mucosa, three general mucosa and two skin markers. The assay has good sensitivity as full RNA profiles for blood, semen and saliva were obtained when using ≥0.05 μL body fluid starting material whereas full DNA profiles were obtained with ≥0.1 μL. We investigated the specificity of the markers by analysing 15 different sets of each type of body fluid and skin with each set consisting of 8 individuals. Since skin markers have not been incorporated in multiplex endpoint PCR assays previously, we analysed these markers in more detail. Interestingly, both skin markers gave a positive result in samplings of the hands, feet, back and lips but negative in tongue samplings. Positive identification (regarding both DNA- and RNA-profiling) was obtained for specimens stored for many years, e.g. blood (28 years-old), semen (28 years-old), saliva (6 years-old), skin (10 years-old) and menstrual secretion (4 years-old). The described approach of combined DNA- and RNA-profiling of body fluids and contact traces assists in the interpretation of forensic stains by providing information about not only the donor(s) that contributed to the stain but also by indicating which cell

  8. A multiplex (m)RNA-profiling system for the forensic identification of body fluids and contact traces.

    PubMed

    Lindenbergh, Alexander; de Pagter, Mirjam; Ramdayal, Geeta; Visser, Mijke; Zubakov, Dmitry; Kayser, Manfred; Sijen, Titia

    2012-09-01

    In current forensic practice, information about the possible biological origin of forensic traces is mostly determined using protein-based presumptive testing. Recently, messenger RNA-profiling has emerged as an alternative strategy to examine the biological origin. Here we describe the development of a single multiplex mRNA-based system for the discrimination of the most common forensic body fluids as well as skin cells. A DNA/RNA co-isolation protocol was established that results in DNA yields equivalent to our standard in-house validated DNA extraction procedure which uses silica-based columns. An endpoint RT-PCR assay was developed that simultaneously amplifies 19 (m)RNA markers. This multiplex assay analyses three housekeeping, three blood, two saliva, two semen, two menstrual secretion, two vaginal mucosa, three general mucosa and two skin markers. The assay has good sensitivity as full RNA profiles for blood, semen and saliva were obtained when using ≥0.05 μL body fluid starting material whereas full DNA profiles were obtained with ≥0.1 μL. We investigated the specificity of the markers by analysing 15 different sets of each type of body fluid and skin with each set consisting of 8 individuals. Since skin markers have not been incorporated in multiplex endpoint PCR assays previously, we analysed these markers in more detail. Interestingly, both skin markers gave a positive result in samplings of the hands, feet, back and lips but negative in tongue samplings. Positive identification (regarding both DNA- and RNA-profiling) was obtained for specimens stored for many years, e.g. blood (28 years-old), semen (28 years-old), saliva (6 years-old), skin (10 years-old) and menstrual secretion (4 years-old). The described approach of combined DNA- and RNA-profiling of body fluids and contact traces assists in the interpretation of forensic stains by providing information about not only the donor(s) that contributed to the stain but also by indicating which cell

  9. Design and testing of an all-digital readout integrated circuit for infrared focal plane arrays

    NASA Astrophysics Data System (ADS)

    Kelly, Michael; Berger, Robert; Colonero, Curtis; Gregg, Mark; Model, Joshua; Mooney, Daniel; Ringdahl, Eric

    2005-08-01

    The digital focal plane array (DFPA) project demonstrates the enabling technologies necessary to build readout integrated circuits for very large infrared focal plane arrays (IR FPAs). Large and fast FPAs are needed for a new class of spectrally diverse sensors. Because of the requirement for high-resolution (low noise) sampling, and because of the sample rate needed for rapid acquisition of high-resolution spectra, it is highly desirable to perform analog-to-digital (A/D) conversion right at the pixel level. A dedicated A/D converter located under every pixel in a one-million-plus element array, and all-digital readout integrated circuits will enable multi- and hyper-spectral imaging systems with unprecedented spatial and spectral resolution and wide area coverage. DFPAs provide similar benefits to standard IR imaging systems as well. We have addressed the key enabling technologies for realizing the DFPA architecture in this work. Our effort concentrated on demonstrating a 60-micron footprint, 14-bit A/D converter and 2.5 Gbps, 16:1 digital multiplexer, the most basic components of the sensor. The silicon test chip was fabricated in a 0.18-micron CMOS process, and was designed to operate with HgxCd1-xTe detectors at cryogenic temperatures. Two A/D designs, one using static logic and one using dynamic logic, were built and tested for performance and power dissipation. Structures for evaluating the bit-error-rate of the multiplexer on-chip and through a differential output driver were implemented for a complete performance assessment. A unique IC probe card with fixtures to mount into an evacuated, closed-cycle helium dewar were also designed for testing up to 2.5 Gbps at temperatures as low as 50 K.

  10. Demountable readout technologies for optical image intensifiers

    NASA Astrophysics Data System (ADS)

    Lapington, J. S.; Howorth, J. R.; Milnes, J. S.

    2007-04-01

    We describe a generic microchannel plate intensifier design for use with a variety of demountable readout devices manufactured using standard multi-layer PCB techniques. We present results obtained using a 50 Ω multi-element design optimized for high speed operation and a four electrode multi-layer device developed from the wedge and strip anode with enhanced image resolution. The benefits of this intensifier design are discussed and a project to develop a detector system for bio-medical applications using a demountable readout device with integrated multi-channel ASIC-based electronics is announced.

  11. A Multiplexed, Two-Electrode Platform for Biosensing based on DNA-Mediated Charge Transport

    PubMed Central

    Furst, Ariel L.; Hill, Michael G.; Barton, Jacqueline K.

    2015-01-01

    We have developed a thin layer, multiplexed biosensing platform that features two working-electrode arrays for detecting small molecules, nucleic acid sequences, and DNA-binding proteins. DNA duplexes are patterned onto the primary electrode array, while a secondary electrode array is used both to initiate DNA monolayer formation, and for electrochemical readout via DNA-mediated charge transport (DNA CT) chemistry. Electrochemical reduction of Cu(phendione)22+ (phendione is 1,10-phenanthroline-5,6-dione) at the secondary electrodes induces covalent attachment via click chemistry of ethynyl-labeled DNA probe duplexes onto the primary electrodes that have been treated with azide-terminated alkythiols. Electrochemical impedance spectroscopy and cyclic voltammetry confirm that catalyst activation at the secondary electrode is essential to maintain the integrity of the DNA monolayer. Electrochemical readout of DNA CT processes that occur at the primary electrode is accomplished at the secondary electrode. The two-electrode system enables the platform to function as a collector-generator using either ferrocyanide or ferricyanide as mediators with methylene blue and DNA charge transport. Electrochemical measurements at the secondary electrode eliminate the need for large background corrections. The resulting sensitivity of this platform enables the reliable and simultaneous detection of femtomoles of the transcription factors TATA-binding protein and CopG on a single multiplexed device. PMID:26042916

  12. A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport.

    PubMed

    Furst, Ariel L; Hill, Michael G; Barton, Jacqueline K

    2015-06-16

    We have developed a thin layer, multiplexed biosensing platform that features two working-electrode arrays for detecting small molecules, nucleic acid sequences, and DNA-binding proteins. DNA duplexes are patterned onto the primary electrode array, while a secondary electrode array is used both to initiate DNA monolayer formation and for electrochemical readout via DNA-mediated charge transport (DNA CT) chemistry. Electrochemical reduction of Cu(phendione)2(2+) (phendione is 1,10-phenanthroline-5,6-dione) at the secondary electrodes induces covalent attachment via click chemistry of ethynyl-labeled DNA probe duplexes onto the primary electrodes that have been treated with azide-terminated alkylthiols. Electrochemical impedance spectroscopy and cyclic voltammetry confirm that catalyst activation at the secondary electrode is essential to maintain the integrity of the DNA monolayer. Electrochemical readout of DNA CT processes that occur at the primary electrode is accomplished also at the secondary electrode. The two-electrode system enables the platform to function as a collector-generator using either ferrocyanide or ferricyanide as mediators with methylene blue and DNA charge transport. Electrochemical measurements at the secondary electrode eliminate the need for large background corrections. The resulting sensitivity of this platform enables the reliable and simultaneous detection of femtomoles of the transcription factors TATA-binding protein and CopG on a single multiplexed device.

  13. Efficient exploration of multiplex networks

    NASA Astrophysics Data System (ADS)

    Battiston, Federico; Nicosia, Vincenzo; Latora, Vito

    2016-04-01

    Efficient techniques to navigate networks with local information are fundamental to sample large-scale online social systems and to retrieve resources in peer-to-peer systems. Biased random walks, i.e. walks whose motion is biased on properties of neighbouring nodes, have been largely exploited to design smart local strategies to explore a network, for instance by constructing maximally mixing trajectories or by allowing an almost uniform sampling of the nodes. Here we introduce and study biased random walks on multiplex networks, graphs where the nodes are related through different types of links organised in distinct and interacting layers, and we provide analytical solutions for their long-time properties, including the stationary occupation probability distribution and the entropy rate. We focus on degree-biased random walks and distinguish between two classes of walks, namely those whose transition probability depends on a number of parameters which is extensive in the number of layers, and those whose motion depends on intrinsically multiplex properties of the neighbouring nodes. We analyse the effect of the structure of the multiplex network on the steady-state behaviour of the walkers, and we find that heterogeneous degree distributions as well as the presence of inter-layer degree correlations and edge overlap determine the extent to which a multiplex can be efficiently explored by a biased walk. Finally we show that, in real-world multiplex transportation networks, the trade-off between efficient navigation and resilience to link failure has resulted into systems whose diffusion properties are qualitatively different from those of appropriately randomised multiplex graphs. This fact suggests that multiplexity is an important ingredient to include in the modelling of real-world systems.

  14. A low-cost, multiplexed μECoG system for high-density recordings in freely moving rodents

    NASA Astrophysics Data System (ADS)

    Insanally, Michele; Trumpis, Michael; Wang, Charles; Chiang, Chia-Han; Woods, Virginia; Palopoli-Trojani, Kay; Bossi, Silvia; Froemke, Robert C.; Viventi, Jonathan

    2016-04-01

    Objective. Micro-electrocorticography (μECoG) offers a minimally invasive neural interface with high spatial resolution over large areas of cortex. However, electrode arrays with many contacts that are individually wired to external recording systems are cumbersome and make recordings in freely behaving rodents challenging. We report a novel high-density 60-electrode system for μECoG recording in freely moving rats. Approach. Multiplexed headstages overcome the problem of wiring complexity by combining signals from many electrodes to a smaller number of connections. We have developed a low-cost, multiplexed recording system with 60 contacts at 406 μm spacing. We characterized the quality of the electrode signals using multiple metrics that tracked spatial variation, evoked-response detectability, and decoding value. Performance of the system was validated both in anesthetized animals and freely moving awake animals. Main results. We recorded μECoG signals over the primary auditory cortex, measuring responses to acoustic stimuli across all channels. Single-trial responses had high signal-to-noise ratios (SNR) (up to 25 dB under anesthesia), and were used to rapidly measure network topography within ∼10 s by constructing all single-channel receptive fields in parallel. We characterized evoked potential amplitudes and spatial correlations across the array in the anesthetized and awake animals. Recording quality in awake animals was stable for at least 30 days. Finally, we used these responses to accurately decode auditory stimuli on single trials. Significance. This study introduces (1) a μECoG recording system based on practical hardware design and (2) a rigorous analytical method for characterizing the signal characteristics of μECoG electrode arrays. This methodology can be applied to evaluate the fidelity and lifetime of any μECoG electrode array. Our μECoG-based recording system is accessible and will be useful for studies of perception and decision

  15. A low-cost, multiplexed μECoG system for high-density recordings in freely-moving rodents

    PubMed Central

    Wang, Charles; Chiang, Chia-Han; Woods, Virginia; Palopoli-Trojani, Kay; Bossi, Silvia; Froemke, Robert C.; Viventi, Jonathan

    2016-01-01

    Objective Micro-electrocorticography (μECoG) offers a minimally invasive neural interface with high spatial resolution over large areas of cortex. However, electrode arrays with many contacts that are individually wired to external recording systems are cumbersome and make recordings in freely-behaving rodents challenging. We report a novel high-density 60-electrode system for μECoG recording in freely-moving rats. Approach Multiplexed headstages overcome the problem of wiring complexity by combining signals from many electrodes to a smaller number of connections We have developed a low-cost, multiplexed recording system with 60 contacts at 406 μm spacing. We characterized the quality of the electrode signals using multiple metrics that tracked spatial variation, evoked-response detectability, and decoding value. Performance of the system was validated both in anesthetized animals and freely-moving awake animals. Main results We recorded μECoG signals over the primary auditory cortex, measuring responses to acoustic stimuli across all channels. Single-trial responses had high signal-to-noise ratios (up to 25 dB under anesthesia), and were used to rapidly measure network topography within ~10 seconds by constructing all single-channel receptive fields in parallel. We characterized evoked potential amplitudes and spatial correlations across the array in the anesthetized and awake animals. Recording quality in awake animals was stable for at least 30 days. Finally, we used these responses to accurately decode auditory stimuli on single trials. Significance This study introduces (1) a μECoG recording system based on practical hardware design and (2) a rigorous analytical method for characterizing the signal characteristics of μECoG electrode arrays. This methodology can be applied to evaluate the fidelity and lifetime of any μECoG electrode array. Our μECoG-based recording system is accessible and will be useful for studies of perception and decision-making in

  16. Measuring and modeling correlations in multiplex networks.

    PubMed

    Nicosia, Vincenzo; Latora, Vito

    2015-09-01

    The interactions among the elementary components of many complex systems can be qualitatively different. Such systems are therefore naturally described in terms of multiplex or multilayer networks, i.e., networks where each layer stands for a different type of interaction between the same set of nodes. There is today a growing interest in understanding when and why a description in terms of a multiplex network is necessary and more informative than a single-layer projection. Here we contribute to this debate by presenting a comprehensive study of correlations in multiplex networks. Correlations in node properties, especially degree-degree correlations, have been thoroughly studied in single-layer networks. Here we extend this idea to investigate and characterize correlations between the different layers of a multiplex network. Such correlations are intrinsically multiplex, and we first study them empirically by constructing and analyzing several multiplex networks from the real world. In particular, we introduce various measures to characterize correlations in the activity of the nodes and in their degree at the different layers and between activities and degrees. We show that real-world networks exhibit indeed nontrivial multiplex correlations. For instance, we find cases where two layers of the same multiplex network are positively correlated in terms of node degrees, while other two layers are negatively correlated. We then focus on constructing synthetic multiplex networks, proposing a series of models to reproduce the correlations observed empirically and/or to assess their relevance. PMID:26465526

  17. Measuring and modeling correlations in multiplex networks

    NASA Astrophysics Data System (ADS)

    Nicosia, Vincenzo; Latora, Vito

    2015-09-01

    The interactions among the elementary components of many complex systems can be qualitatively different. Such systems are therefore naturally described in terms of multiplex or multilayer networks, i.e., networks where each layer stands for a different type of interaction between the same set of nodes. There is today a growing interest in understanding when and why a description in terms of a multiplex network is necessary and more informative than a single-layer projection. Here we contribute to this debate by presenting a comprehensive study of correlations in multiplex networks. Correlations in node properties, especially degree-degree correlations, have been thoroughly studied in single-layer networks. Here we extend this idea to investigate and characterize correlations between the different layers of a multiplex network. Such correlations are intrinsically multiplex, and we first study them empirically by constructing and analyzing several multiplex networks from the real world. In particular, we introduce various measures to characterize correlations in the activity of the nodes and in their degree at the different layers and between activities and degrees. We show that real-world networks exhibit indeed nontrivial multiplex correlations. For instance, we find cases where two layers of the same multiplex network are positively correlated in terms of node degrees, while other two layers are negatively correlated. We then focus on constructing synthetic multiplex networks, proposing a series of models to reproduce the correlations observed empirically and/or to assess their relevance.

  18. Readout of the upgraded ALICE-ITS

    NASA Astrophysics Data System (ADS)

    Szczepankiewicz, A.

    2016-07-01

    The ALICE experiment will undergo a major upgrade during the second long shutdown of the CERN LHC. As part of this program, the present Inner Tracking System (ITS), which employs different layers of hybrid pixels, silicon drift and strip detectors, will be replaced by a completely new tracker composed of seven layers of monolithic active pixel sensors. The upgraded ITS will have more than twelve billion pixels in total, producing 300 Gbit/s of data when tracking 50 kHz Pb-Pb events. Two families of pixel chips realized with the TowerJazz CMOS imaging process have been developed as candidate sensors: the ALPIDE, which uses a proprietary readout and sparsification mechanism and the MISTRAL-O, based on a proven rolling shutter architecture. Both chips can operate in continuous mode, with the ALPIDE also supporting triggered operations. As the communication IP blocks are shared among the two chip families, it has been possible to develop a common Readout Electronics. All the sensor components (analog stages, state machines, buffers, FIFOs, etc.) have been modelled in a system level simulation, which has been extensively used to optimize both the sensor and the whole readout chain design in an iterative process. This contribution covers the progress of the R&D efforts and the overall expected performance of the ALICE-ITS readout system.

  19. Structural measures for multiplex networks

    NASA Astrophysics Data System (ADS)

    Battiston, Federico; Nicosia, Vincenzo; Latora, Vito

    2014-03-01

    Many real-world complex systems consist of a set of elementary units connected by relationships of different kinds. All such systems are better described in terms of multiplex networks, where the links at each layer represent a different type of interaction between the same set of nodes rather than in terms of (single-layer) networks. In this paper we present a general framework to describe and study multiplex networks, whose links are either unweighted or weighted. In particular, we propose a series of measures to characterize the multiplexicity of the systems in terms of (i) basic node and link properties such as the node degree, and the edge overlap and reinforcement, (ii) local properties such as the clustering coefficient and the transitivity, and (iii) global properties related to the navigability of the multiplex across the different layers. The measures we introduce are validated on a genuinely multiplex data set of Indonesian terrorists, where information among 78 individuals are recorded with respect to mutual trust, common operations, exchanged communications, and business relationships.

  20. Structural measures for multiplex networks.

    PubMed

    Battiston, Federico; Nicosia, Vincenzo; Latora, Vito

    2014-03-01

    Many real-world complex systems consist of a set of elementary units connected by relationships of different kinds. All such systems are better described in terms of multiplex networks, where the links at each layer represent a different type of interaction between the same set of nodes rather than in terms of (single-layer) networks. In this paper we present a general framework to describe and study multiplex networks, whose links are either unweighted or weighted. In particular, we propose a series of measures to characterize the multiplexicity of the systems in terms of (i) basic node and link properties such as the node degree, and the edge overlap and reinforcement, (ii) local properties such as the clustering coefficient and the transitivity, and (iii) global properties related to the navigability of the multiplex across the different layers. The measures we introduce are validated on a genuinely multiplex data set of Indonesian terrorists, where information among 78 individuals are recorded with respect to mutual trust, common operations, exchanged communications, and business relationships.

  1. Screening Reactive Metabolites Bioactivated by Multiple Enzyme Pathways Using a Multiplexed Microfluidic System

    PubMed Central

    Wasalathanthri, Dhanuka P.; Faria, Ronaldo C.; Malla, Spundana; Joshi, Amit A.; Schenkman, John B.; Rusling, James F.

    2012-01-01

    A multiplexed, microfluidic platform to detect reactive metabolites is described, and its performance is illustrated for compounds metabolized by oxidative and bioconjugation enzymes in multi-enzyme pathways to mimic natural human drug metabolism. The device features four 8-electrode screen printed carbon arrays coated with thin films of DNA, a ruthenium-polyvinylpyridine (RuPVP) catalyst, and multiple enzyme sources including human liver microsomes (HLM), cytochrome P450 (cyt P450) 1B1 supersomes, microsomal epoxide hydrolase (EH), human S9 liver fractions (Hs9) and N-acetyltransferase (NAT). Arrays are arranged in parallel to facilitate multiple compound screening, enabling up to 32 enzyme reactions and measurements in 20–30 min. In the first step of the assay, metabolic reactions are achieved under constant flow of oxygenated reactant solutions by electrode driven natural catalytic cycles of cyt P450s and cofactor-supported bioconjugation enzymes. Reactive metabolites formed in the enzyme reactions can react with DNA. Relative DNA damage is measuring in the second assay step using square wave voltammetry (SWV) with RuPVP as catalyst. Studies were done on chemicals known to require metabolic activation to induce genotoxicity, and results reproduced known features of metabolite DNA-reactivity for the test compounds. Metabolism of benzo[a]pyrene (B[a]P) by cyt P450s and epoxide hydrolase showed an enhanced relative DNA damage rate for DNA damage compared to cyt P450s alone. DNA damage rates for arylamines by pathways featuring both oxidative and conjugative enzymes at pH 7.4 gave better correlation with rodent genotoxicity metric TD50. Results illustrate the broad utility of the reactive metabolite screening device. PMID:23095952

  2. Screening reactive metabolites bioactivated by multiple enzyme pathways using a multiplexed microfluidic system.

    PubMed

    Wasalathanthri, Dhanuka P; Faria, Ronaldo C; Malla, Spundana; Joshi, Amit A; Schenkman, John B; Rusling, James F

    2013-01-01

    A multiplexed, microfluidic platform to detect reactive metabolites is described, and its performance is illustrated for compounds metabolized by oxidative and bioconjugation enzymes in multi-enzyme pathways to mimic natural human drug metabolism. The device features four 8-electrode screen printed carbon arrays coated with thin films of DNA, a ruthenium-polyvinylpyridine (RuPVP) catalyst, and multiple enzyme sources including human liver microsomes (HLM), cytochrome P450 (cyt P450) 1B1 supersomes, microsomal epoxide hydrolase (EH), human S9 liver fractions (Hs9) and N-acetyltransferase (NAT). Arrays are arranged in parallel to facilitate multiple compound screening, enabling up to 32 enzyme reactions and measurements in 20-30 min. In the first step of the assay, metabolic reactions are achieved under constant flow of oxygenated reactant solutions by electrode driven natural catalytic cycles of cyt P450s and cofactor-supported bioconjugation enzymes. Reactive metabolites formed in the enzyme reactions can react with DNA. Relative DNA damage is measured in the second assay step using square wave voltammetry (SWV) with RuPVP as catalyst. Studies were done on chemicals known to require metabolic activation to induce genotoxicity, and results reproduced known features of metabolite DNA-reactivity for the test compounds. Metabolism of benzo[a]pyrene (B[a]P) by cyt P450s and epoxide hydrolase showed an enhanced relative DNA damage rate for DNA compared to cyt P450s alone. DNA damage rates for arylamines by pathways featuring both oxidative and conjugative enzymes at pH 7.4 gave better correlation with rodent genotoxicity metric TD(50). Results illustrate the broad utility of the reactive metabolite screening device.

  3. A reconfigurable image tube using an external electronic image readout

    NASA Astrophysics Data System (ADS)

    Lapington, J. S.; Howorth, J. R.; Milnes, J. S.

    2005-08-01

    We have designed and built a sealed tube microchannel plate (MCP) intensifier for optical/NUV photon counting applications suitable for 18, 25 and 40 mm diameter formats. The intensifier uses an electronic image readout to provide direct conversion of event position into electronic signals, without the drawbacks associated with phosphor screens and subsequent optical detection. The Image Charge technique is used to remove the readout from the intensifier vacuum enclosure, obviating the requirement for additional electrical vacuum feedthroughs and for the readout pattern to be UHV compatible. The charge signal from an MCP intensifier is capacitively coupled via a thin dielectric vacuum window to the electronic image readout, which is external to the sealed intensifier tube. The readout pattern is a separate item held in proximity to the dielectric window and can be easily detached, making the system easily reconfigurable. Since the readout pattern detects induced charge and is external to the tube, it can be constructed as a multilayer, eliminating the requirement for narrow insulator gaps and allowing it to be constructed using standard PCB manufacturing tolerances. We describe two readout patterns, the tetra wedge anode (TWA), an optimized 4 electrode device similar to the wedge and strip anode (WSA) but with a factor 2 improvement in resolution, and an 8 channel high speed 50 ohm device, both manufactured as multilayer PCBs. We present results of the detector imaging performance, image resolution, linearity and stability, and discuss the development of an integrated readout and electronics device based on these designs.

  4. Free-Space optical interconnects for cable-less readout in particle physics detectors

    SciTech Connect

    Chramowicz, John; Kwan, Simon; Moretti, Tony; Sugg, Alan; Prosser, Alan; /Fermilab

    2010-10-01

    Particle physics detectors utilize readout data links requiring a complicated network of copper wires or optical fibers. These links are both massive and costly. Upgrades to such detectors may require additional bandwidth to be provisioned with limited space available to route new cables or fibers. In contrast, free-space optical interconnects will offer cable-less readout, thereby resulting in significant reductions of material and labor. A collaborative effort between Fermilab and Vega Wave Systems is pursuing the development of a unique free-space optical link design that utilizes the transparency of silicon at wavelengths including 1310 nm and multiple wavelengths used in standard telecommunications applications such as coarse wavelength division multiplexing (CWDM). The first step in the pursuit of that design is a proof that the concept may be viable. To that end, experiments have been performed to characterize the bit error rate performance of a prototype link over a free-space optical path and through doped silicon at multi-gigabit rates. These experiments have demonstrated that operation within acceptable bit error rates is possible using single and multiple wavelength transmission arrangements.

  5. Equivalence of time-multiplexed and frequency-multiplexed signals in digital communications.

    NASA Technical Reports Server (NTRS)

    Timor, U.

    1972-01-01

    In comparing different techniques for multiplexing N binary data signals into a single channel, time-division multiplexing (TDM) is known to have a theoretic efficiency of 100 percent (neglecting sync power) and thus seems to outperform frequency-division multiplexing systems (FDM). By considering more general FDM systems, we will show that both TDM and FDM are equivalent and have an efficiency of 100 percent. The difference between the systems is in the multiplexing and demultiplexing subsystems, but not in the performance or in the generated waveforms.

  6. Effects of using the GlobalFiler™ multiplex system on parent-child analyses of cases with single locus inconsistency.

    PubMed

    Ochiai, Eriko; Osawa, Motoki; Tamura, Tomonori; Minaguchi, Kiyoshi; Miyashita, Keiko; Matsushima, Yutaka; Kakimoto, Yu; Satoh, Fumiko

    2016-01-01

    Parent-child analyses sometimes reveal inconsistency of shared alleles at only one locus. This is conventionally called "single locus exclusion", which results from mutational events and the presence of null alleles. Here, in parent-child analyses of the Japanese population, we detected exclusions by using the GlobalFiler™ system comprising 21 short tandem repeat loci. One- or two-step mutations resulting from strand slippage causing gain or loss were observed in seven of 221 parent-child transmissions. The incidences of single locus inconsistency of alleles were 5.88×10(-2) and 8.40×10(-3) for paternal and maternal relationships, respectively. With calculation using a set of 15 loci in the Identifiler® multiplex system, the combined likelihood ratio (CLR) values were limited to less than 100 in all five cases accompanied by single inconsistency. The addition of six loci recovered the CLR values to over 10,000 in three cases. Application of this advanced system may increase the detected occurrence of mutational events, but it should be beneficial for inference in parent-child analyses, particularly in cases accompanied by genetic inconsistency.

  7. Thermally multiplexed polymerase chain reaction.

    PubMed

    Phaneuf, Christopher R; Pak, Nikita; Saunders, D Curtis; Holst, Gregory L; Birjiniuk, Joav; Nagpal, Nikita; Culpepper, Stephen; Popler, Emily; Shane, Andi L; Jerris, Robert; Forest, Craig R

    2015-07-01

    Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously-each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel. PMID:26339317

  8. Thermally multiplexed polymerase chain reaction

    PubMed Central

    Phaneuf, Christopher R.; Pak, Nikita; Saunders, D. Curtis; Holst, Gregory L.; Birjiniuk, Joav; Nagpal, Nikita; Culpepper, Stephen; Popler, Emily; Shane, Andi L.; Jerris, Robert; Forest, Craig R.

    2015-01-01

    Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel. PMID:26339317

  9. The genotyping of infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction.

    PubMed

    Huang, Shr-Wei; Ho, Chia-Fang; Chan, Kun-Wei; Cheng, Min-Chung; Shien, Jui-Hung; Liu, Hung-Jen; Wang, Chi-Young

    2014-11-01

    Infectious bronchitis virus (IBV; Avian coronavirus) causes acute respiratory and reproductive and urogenital diseases in chickens. Following sequence alignment of IBV strains, a combination of selective primer sets was designed to individually amplify the IBV wild-type and vaccine strains using a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (ARMS RT-PCR) approach. This system was shown to discriminate the IBV wild-type and vaccine strains. Moreover, an ARMS real-time RT-PCR (ARMS qRT-PCR) was combined with a high-resolution analysis (HRMA) to establish a melt curve analysis program. The specificity of the ARMS RT-PCR and the ARMS qRT-PCR was verified using unrelated avian viruses. Different melting temperatures and distinct normalized and shifted melting curve patterns for the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were detected. The new assays were used on samples of lung and trachea as well as virus from allantoic fluid and cell culture. In addition to being able to detect the presence of IBV vaccine and wild-type strains by ARMS RT-PCR, the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were distinguished using ARMS qRT-PCR by their melting temperatures and by HRMA. These approaches have acceptable sensitivities and specificities and therefore should be able to serve as options when carrying out differential diagnosis of IBV in Taiwan and China.

  10. Multi-channel detector readout method and integrated circuit

    DOEpatents

    Moses, William W.; Beuville, Eric; Pedrali-Noy, Marzio

    2004-05-18

    An integrated circuit which provides multi-channel detector readout from a detector array. The circuit receives multiple signals from the elements of a detector array and compares the sampled amplitudes of these signals against a noise-floor threshold and against one another. A digital signal is generated which corresponds to the location of the highest of these signal amplitudes which exceeds the noise floor threshold. The digital signal is received by a multiplexing circuit which outputs an analog signal corresponding the highest of the input signal amplitudes. In addition a digital control section provides for programmatic control of the multiplexer circuit, amplifier gain, amplifier reset, masking selection, and test circuit functionality on each input thereof.

  11. Multi-channel detector readout method and integrated circuit

    DOEpatents

    Moses, William W.; Beuville, Eric; Pedrali-Noy, Marzio

    2006-12-12

    An integrated circuit which provides multi-channel detector readout from a detector array. The circuit receives multiple signals from the elements of a detector array and compares the sampled amplitudes of these signals against a noise-floor threshold and against one another. A digital signal is generated which corresponds to the location of the highest of these signal amplitudes which exceeds the noise floor threshold. The digital signal is received by a multiplexing circuit which outputs an analog signal corresponding the highest of the input signal amplitudes. In addition a digital control section provides for programmatic control of the multiplexer circuit, amplifier gain, amplifier reset, masking selection, and test circuit functionality on each input thereof.

  12. PANDA straw tube detectors and readout

    NASA Astrophysics Data System (ADS)

    Strzempek, P.

    2016-07-01

    PANDA is a detector under construction dedicated to studies of production and interaction of particles in the charmonium mass range using antiproton beams in the momentum range of 1.5 - 15 GeV/c at the Facility for Antiproton and Ion Research (FAIR) in Darmstadt. PANDA consists of two spectrometers: a Target Spectrometer with a superconducting solenoid and a Forward Spectrometer using a large dipole magnet and covering the most forward angles (Θ < 10 °). In both spectrometers, the particle's trajectories in the magnetic field are measured using self-supporting straw tube detectors. The expected high count rates, reaching up to 1 MHz/straw, are one of the main challenges for the detectors and associated readout electronics. The paper presents the readout chain of the tracking system and the results of tests performed with realistic prototype setups. The readout chain consists of a newly developed ASIC chip (PASTTREC < PANDASTTReadoutChip >) with amplification, signal shaping, tail cancellation, discriminator stages and Time Readout Boards as digitizer boards.

  13. Raman-based microarray readout: a review.

    PubMed

    Haisch, Christoph

    2016-07-01

    For a quarter of a century, microarrays have been part of the routine analytical toolbox. Label-based fluorescence detection is still the commonest optical readout strategy. Since the 1990s, a continuously increasing number of label-based as well as label-free experiments on Raman-based microarray readout concepts have been reported. This review summarizes the possible concepts and methods and their advantages and challenges. A common label-based strategy is based on the binding of selective receptors as well as Raman reporter molecules to plasmonic nanoparticles in a sandwich immunoassay, which results in surface-enhanced Raman scattering signals of the reporter molecule. Alternatively, capture of the analytes can be performed by receptors on a microarray surface. Addition of plasmonic nanoparticles again leads to a surface-enhanced Raman scattering signal, not of a label but directly of the analyte. This approach is mostly proposed for bacteria and cell detection. However, although many promising readout strategies have been discussed in numerous publications, rarely have any of them made the step from proof of concept to a practical application, let alone routine use. Graphical Abstract Possible realization of a SERS (Surface-Enhanced Raman Scattering) system for microarray readout. PMID:26973235

  14. Dual Common Planes for Time Multiplexing of Dual-Color QWIPs

    NASA Technical Reports Server (NTRS)

    Rafol, Sir B.; Gunapala, Srath; Bandara, Sumith; Liu, John; Mumolo, Jason

    2006-01-01

    A proposed improved method of externally controlled time multiplexing of the readouts of focal-plane arrays of pairs of stacked quantum-well infrared photodetectors (QWIPs) that operate in different wavelength bands is based on a dual-detector-common-plane circuit configuration. The method would be implemented in a QWIP integrated-circuit chip hybridized with a readout integrated-circuit (ROIC) chip.

  15. Environmental monitoring for biological threat agents using the autonomous pathogen detection system with multiplexed polymerase chain reaction.

    PubMed

    Regan, John F; Makarewicz, Anthony J; Hindson, Benjamin J; Metz, Thomas R; Gutierrez, Dora M; Corzett, Todd H; Hadley, Dean R; Mahnke, Ryan C; Henderer, Bruce D; Breneman, John W; Weisgraber, Todd H; Dzenitis, John M

    2008-10-01

    We have developed and field-tested a now operational civilian biodefense capability that continuously monitors the air in high-risk locations for biological threat agents. This stand-alone instrument, called the Autonomous Pathogen Detection System (APDS), collects and selectively concentrates particles from the air into liquid samples and analyzes the samples using multiplexed PCR amplification coupled with microsphere array detection. During laboratory testing, we evaluated the APDS instrument's response to Bacillus anthracis and Yersinia pestis by spiking the liquid sample stream with viable spores and cells, bead-beaten lysates, and purified DNA extracts. APDS results were also compared to a manual real-time PCR method. Field data acquired during 74 days of continuous operation at a mass-transit subway station are presented to demonstrate the specificity and reliability of the APDS. The U.S. Department of Homeland Security recently selected the APDS reported herein as the first autonomous detector component of their BioWatch antiterrorism program. This sophisticated field-deployed surveillance capability now generates actionable data in one-tenth the time of manual filter collection and analysis. PMID:18763806

  16. In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system

    PubMed Central

    Narayanan, Anand; Hill-Teran, Guillermina; Moro, Albertomaria; Ristori, Emma; Kasper, Dionna M.; A. Roden, Christine; Lu, Jun; Nicoli, Stefania

    2016-01-01

    A large number of microRNAs (miRNAs) are grouped into families derived from the same phylogenetic ancestors. miRNAs within a family often share the same physiological functions despite differences in their primary sequences, secondary structures, or chromosomal locations. Consequently, the generation of animal models to analyze the activity of miRNA families is extremely challenging. Using zebrafish as a model system, we successfully provide experimental evidence that a large number of miRNAs can be simultaneously mutated to abrogate the activity of an entire miRNA family. We show that injection of the Cas9 nuclease and two, four, ten, and up to twenty-four multiplexed single guide RNAs (sgRNAs) can induce mutations in 90% of the miRNA genomic sequences analyzed. We performed a survey of these 45 mutations in 10 miRNA genes, analyzing the impact of our mutagenesis strategy on the processing of each miRNA both computationally and in vivo. Our results offer an effective approach to mutate and study the activity of miRNA families and pave the way for further analysis on the function of complex miRNA families in higher multicellular organisms. PMID:27572667

  17. Development of a single multiplex amplification refractory mutation system PCR for the detection of rifampin-resistant Mycobacterium tuberculosis.

    PubMed

    Shi, Xiaodan; Zhang, Chen; Shi, Ming; Yang, Mengjie; Zhang, Yi; Wang, Ji; Shen, Hongwei; Zhao, Gang; Ma, Xuejun

    2013-11-01

    A rapid and simple method for the detection of drug-resistant Mycobacterium tuberculosis is critical for the efficient treatment and control of this pathogen in developing country. Here we developed a single multiplex amplification refractory mutation system (M-ARMS) PCR, in which chimeric-primer and temperature switch PCR (TSP) strategy were included. Using this method, we detected rifampin resistance-associated mutations at codons 511, 516, 526 and 531 in the rifampin resistance-determining region of rpoB gene. The performance of M-ARMS-PCR assay was evaluated with 135 cultured isolates of M. tuberculosis. The sensitivity and specificity were 94.2% and 100%, respectively, compared with direct DNA sequencing, and 86.67% and 89.71%, respectively, compared with culture-based phenotypic drug susceptibility testing. Therefore, this newly-developed M-ARMS-PCR method is useful and efficient with an intended application in provincial Centers for Disease Control and Prevention for rapid detection of rifampin resistance-associated mutations.

  18. Genotyping of Pseudomonas aeruginosa Type III Secretion System Using Magnetic Enrichment Multiplex Polymerase Chain Reaction and Chemiluminescence.

    PubMed

    Tang, Yongjun; Li, Bo; Dai, Jianguo; Dai, Jianfang; Wang, Xinhui; Si, Jing; Ali, Zeeshan; Li, Taotao; He, Nongyue

    2016-04-01

    The pathologic characteristics and toxicity mechanism of Pseudomonas aeruginosa are different in strains with different Type III secretion system (T3SS) genes. The T3SS gene based genotyping of P. aeruginosa strains is important to understand its virulence and predict the clinical outcomes. In this study, a rapid and automatable method for T3SS genotyping was developed using magnetic enrichment multiplex PCR and chemiluminescence. Three P. aeruginosa standard strains were analyzed using this method. The results showed that the chemiluminescent intensity of exoT, exoY, and exoS of these strains were 10 times greater than that of the control, and that their Q values were greater than 2.1. These results were consistent with the regular PCR and electrophoresis results, indicating that the method was reliable. Out of the 22 clinical isolates tested using this method, 100%, 72.7%, 95.5%, and 4.5% of the isolates contained exoT, exoY, exoS, and exoU genes, respectively. The isolates harbored either exoS or exoU gene, but not both. All genotyping results of the isolates were consistent with the information obtained using regular PCR and electrophoresis. PMID:27301202

  19. Turing patterns in multiplex networks

    NASA Astrophysics Data System (ADS)

    Asllani, Malbor; Busiello, Daniel M.; Carletti, Timoteo; Fanelli, Duccio; Planchon, Gwendoline

    2014-10-01

    The theory of patterns formation for a reaction-diffusion system defined on a multiplex is developed by means of a perturbative approach. The interlayer diffusion constants act as a small parameter in the expansion and the unperturbed state coincides with the limiting setting where the multiplex layers are decoupled. The interaction between adjacent layers can seed the instability of a homogeneous fixed point, yielding self-organized patterns which are instead impeded in the limit of decoupled layers. Patterns on individual layers can also fade away due to cross-talking between layers. Analytical results are compared to direct simulations.

  20. Design of a 12-bit 1 MS/s SAR-ADC for front-end readout of 32-channel CZT detector imaging system

    NASA Astrophysics Data System (ADS)

    Liu, Wei; Wei, Tingcun; Li, Bo; Guo, Panjie; Hu, Yongcai

    2015-06-01

    A 12-bit 1MS/s SAR-ADC for the front-end readout of a 32-channel CZT detector imaging system is presented. In order to improve the performances of the ADC, several techniques are proposed. First, a novel offset cancellation method for comparator is proposed, in which no any capacitor is introduced in the signal pathway, thus it has faster operation speed than traditional one. Second, the architecture of unit capacitor array is adopted in the charge-redistribution DAC to reduce the capacitor mismatch. Third, the radiation-hardened ability is enhanced through circuit and layout design. The prototype chip was fabricated using a TSMC 0.35 um 2P4M CMOS process. At a 3.3/5 V power supply, the proposed SAR-ADC achieves 67.64 dB SINAD at 1MS/s, consumes 10 mW power and occupies a core area of 1180×1080 um2.

  1. Autoantibodies Profile in the Sera of Patients with Sjogren]s Syndrome: The ANA Evaluation—A Homogeneous, Multiplexed System

    PubMed Central

    Gilburd, Boris; Abu-Shakra, Mahmoud; Giordano, Andrea; Bocci, Elena Bartoloni; delle Monache, Francesco; Gerli, Roberto

    2004-01-01

    Background: Flow-based, multiplex bead arrays (MBA) have been developed for a variety of applications including the detection of antibodies to extractable nuclear antigens (ENA). It offers a rapid and sensitive method to assess multiple analyses in a single tube/well. Purpose: To evaluate the Athena Multi-Lyte ANA Test System utilizes Luminex Corporation's MBA technology for the detection of antinuclear antibodies (ANA) and ENA antibodies in the sera of patients with Sjogren's syndrome (SS). Methods: MBA assay was used to detect ANA and ENA antibodies in the sera of 37 patients with SS and 96 sera from healthy subjects. Results: All patients were women. Their mean age was 48.7 years and the mean disease duration was 7.27 years. ANA was found in 3 (3%) sera of healthy subjects by the AtheNA system and in 2 (2%) sera by the ELISA kit. A 99% concordance between the 2 assays was found. A 94.6% concordance between the 2 assays was found by testing the sera of patients with SS for ANA. By the AtheNA system, none of the sera of 37 patients with SS had autoantibodies reacting with Sm, Jo-1, dsDNA or histones. Anti-RNP antibody was found in 5.4% of the sera and 2.7% of the sera reacted with Scl-70 and histones. Anti-SS/A and anti-SS/B were identified in 84 and 76% of the sera, respectively. Conclusion: The AtheNa Multi-Lyte ANA Test System offers a sensitive and specific result for the detection of ANA and ENA antibodies in the sera of patients with SS. PMID:15154612

  2. Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

    PubMed Central

    Dobnik, David; Štebih, Dejan; Blejec, Andrej; Morisset, Dany; Žel, Jana

    2016-01-01

    The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets. PMID:27739510

  3. Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

    NASA Astrophysics Data System (ADS)

    Dobnik, David; Štebih, Dejan; Blejec, Andrej; Morisset, Dany; Žel, Jana

    2016-10-01

    The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets.

  4. Multiplexed detection and differentiation of the DNA strains for influenza A (H1N1 2009) using a silicon-based microfluidic system.

    PubMed

    Kao, Linus Tzu-Hsiang; Shankar, Lakshmi; Kang, Tae Goo; Zhang, Guojun; Tay, Guang Kai Ignatius; Rafei, Siti Rafeah Mohamed; Lee, Charlie Wah Heng

    2011-01-15

    Pandemic influenza by the swine-origin influenza virus (H1N1 2009) has attracted considerable concern worldwide. A convenient and accurate diagnostic approach that can be deployed at the point of care, such as in a doctor's office or at an airport, is critical for disease control. Here we report the development of a silicon-based microfluidic system for subtype differentiation of the novel H1N1 2009 strain vs. the seasonal influenza A (FluA) strain. The proposed system included two functional modules: a multiplexed PCR module for amplification of nucleic acid targets and a multiplexed silicon nanowire (SiNW) module for sequence determination. The PCR module consisted of a microfluidic PCR chamber and an electrical controller to perform a multiplexed protocol that simultaneously enriched specific segments of both H1N1 and FluA strains (if present), with 10(4)-10(5) amplification efficiency. The PCR amplicon was subsequently denatured and transferred to the SiNW sensing module for a label-free, multiplexed detection. A control SiNW was implemented, for the first time, in order to eliminate background interference. The detection module demonstrated a 10× change in the magnitude of differential current when the target DNA was injected. Overall, the system achieved a sensitivity of 20-30 fg/μl for H1N1 and seasonal FluA nucleic acids in a 10 μl sample. The low sample consumption, high sensitivity and high specificity render it a potential point-of-care (POC) platform to help doctors reach a yes/no decision for infectious diseases.

  5. Characterization of Silicon Detector Readout Electronics

    SciTech Connect

    Jones, M.

    2015-07-22

    Configuration and calibration of the front-end electronics typical of many silicon detector configurations were investigated in a lab activity based on a pair of strip sensors interfaced with FSSR2 read-out chips and an FPGA. This simple hardware configuration, originally developed for a telescope at the Fermilab Test Beam Facility, was used to measure thresholds and noise on individual readout channels and to study the influence that different configurations of the front-end electronics had on the observed levels of noise in the system. An understanding of the calibration and operation of this small detector system provided an opportunity to explore the architecture of larger systems such as those currently in use at LHC experiments.

  6. Remote PCF-based sensors multiplexing by using optical add-drop multiplexers

    NASA Astrophysics Data System (ADS)

    Bravo, Mikel; Candiani, Alessandro; Cucinotta, Annamaria; Selleri, Stefano; Lopez-Amo, Manuel; Kobelke, Jens; Schuster, Kay

    2014-04-01

    A 100 km remote PCF micro-displacement sensor multiplexing system based on optical add-drop multiplexers (OADMs) has been experimentally demonstrated. The PCF sensors are placed in an OADM bus structure which is illuminated by a home-made tunable fiber optic ring laser. Four micro-displacement photonic crystal fiber (PCF) sensors based on a suspended core fiber inserted into a Sagnac loop filter are multiplexed. Furthermore, being the first proposal to solve this issue in PCF sensor multiplexing structures, these sensors can be referenced with an extra wavelength.

  7. Demonstration of simultaneous mode conversion and demultiplexing for mode and wavelength division multiplexing systems based on tilted few-mode fiber Bragg gratings.

    PubMed

    Gao, Ya; Sun, Junqiang; Chen, Guodong; Sima, Chaotan

    2015-04-20

    We experimentally demonstrate mode conversion by exploiting optical reflection of tilted few-mode fiber Bragg grating (FM-FBG). Mode conversions from LP(01) mode to higher symmetric and asymmetric modes are achieved, and more than 99.5% conversion efficiency from LP(01) to LP(11) mode is obtained using a 1.6°-tilted FM-FBG. Influences of the weakly tilted FM-FBG parameters on the property of mode conversion is analyzed and discussed. A simultaneous mode conversion and demultiplexing scheme for 4-mode × 3-wavelength multiplexing transmission is proposed and the modal crosstalk is analyzed based on the transmission spectra of the tilted FM-FBGs. The proposed approach shows potential applications in mode and wavelength division multiplexing communication systems. PMID:25969037

  8. Demonstration of simultaneous mode conversion and demultiplexing for mode and wavelength division multiplexing systems based on tilted few-mode fiber Bragg gratings.

    PubMed

    Gao, Ya; Sun, Junqiang; Chen, Guodong; Sima, Chaotan

    2015-04-20

    We experimentally demonstrate mode conversion by exploiting optical reflection of tilted few-mode fiber Bragg grating (FM-FBG). Mode conversions from LP(01) mode to higher symmetric and asymmetric modes are achieved, and more than 99.5% conversion efficiency from LP(01) to LP(11) mode is obtained using a 1.6°-tilted FM-FBG. Influences of the weakly tilted FM-FBG parameters on the property of mode conversion is analyzed and discussed. A simultaneous mode conversion and demultiplexing scheme for 4-mode × 3-wavelength multiplexing transmission is proposed and the modal crosstalk is analyzed based on the transmission spectra of the tilted FM-FBGs. The proposed approach shows potential applications in mode and wavelength division multiplexing communication systems.

  9. Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applications of the Luminex LabMAP system.

    PubMed

    Dunbar, Sherry A; Vander Zee, Coe A; Oliver, Kerry G; Karem, Kevin L; Jacobson, James W

    2003-05-01

    Escherichia coli, Salmonella, Listeria monocytogenes and Campylobacter jejuni are bacterial pathogens commonly implicated in foodborne illnesses. Generally used detection methods (i.e., culture, biochemical testing, ELISA and nucleic acid amplification) can be laborious, time-consuming and require multiple tests to detect all of the pathogens. Our objective was to develop rapid assays to simultaneously detect these four organisms through the presence of antigen or DNA using the Luminex LabMAP system. For nucleic acid detection, organism-specific capture probes corresponding to the 23S ribosomal RNA gene (rrl) were coupled covalently to LabMAP microspheres. Target molecules included synthetic complementary oligonucleotides and genomic DNA isolated from ATCC type strains or other well-characterized strains of each organism. Universal PCR primers were designed to amplify variable regions of bacterial 23S ribosomal DNA, yielding biotinylated amplicons of 86 to 109 bp in length. Varying quantities of targets were hybridized to the combined microsphere sets, labeled with streptavidin-R-phycoerythrin and analyzed on the Luminex(100) system. Results of nucleic acid detection assays, obtained in 30 to 40 min following amplification, correctly and specifically identified each bacterial species with a detection sensitivity of 10(3) to 10(5) genome copies. Capture-sandwich immunoassays were developed with organism-specific antibodies coupled to different microsphere sets. Microspheres were incubated with organism-specific standards and reactivity was assessed with biotinylated detection antibodies and streptavidin-R-phycoerythrin. In the immunoassays, microsphere-associated fluorescence was organism concentration dependent with detectable response at < or = 1000 organisms/ml and with no apparent cross-reactivity. We have demonstrated that the Luminex LabMAP system is a rapid, flexible platform capable of simultaneous, sensitive and specific detection of pathogens. The

  10. A THIN-LAYER LIF THERMOLUMINESCENCE DOSEMETER SYSTEM WITH FAST READOUT FOR THE USE IN PERSONAL DOSIMETRY SERVICES.

    PubMed

    Walbersloh, J; Busch, F

    2016-09-01

    A newly developed thermoluminescence dosemeter system is presented that is suitable for application in fields where personal monitoring of a large number of users is required. The system presented here is intended to be used as the upcoming main dosemeter for whole body dosimetry at the dosimetry service of the MPA NRW (Germany) with ∼110,000 evaluations per month.

  11. Practical considerations for a four-channel multiplexed Bragg grating fiber sensor system

    NASA Astrophysics Data System (ADS)

    Alavie, A. Tino; Ohn, Myo M.; Glennie, Derek J.; Karr, Shawn E.; Maaskant, Robert; Fishbein, G.; Lee, R.; Huang, Shang Yuan; Measures, Raymond M.

    1994-02-01

    A multichannel Bragg grating fiber laser demodulation system capable of interrogating four or more Bragg grating sensors for strain and temperature monitoring has been designed and developed. System configuration and various practical considerations for a field deployable system are discussed. Preliminary data indicates a dynamic strain range in excess of 5000 (mu) (epsilon) at a resolution of 1 (mu) (epsilon) . Both the strain dynamic range and resolution are easily modified.

  12. Readout Techniques for Drift and Low Frequency Noise Rejection in Infrared Arrays

    NASA Astrophysics Data System (ADS)

    Finger, G.; Dorn, R. J.; Hoffman, A. W.; Mehrgan, H.; Meyer, M.; Moorwood, A. F. M.; Steigmeier, J.

    Three different methods are presented to subtract thermal drifts and low-frequency noise from the signal of infrared array. The first is dead pixels with open Indium bumps, the second is reference output as implemented on the Hawaii2 multiplexer, and the third is dark pixels to emulate reference cells having a capacity connected to the gate of the unit cell field-effect transistor (FET). The third method is the most effective and yields a reduction in readout noise from 15.4-9.4 erms. A novel method will be described to extend this readout technique to the Aladdin 1Kx1K InSb array.

  13. FDM Readout Assembly with Flexible, Superconducting Connection to Cryogenic kilo-Pixel TES Detectors

    NASA Astrophysics Data System (ADS)

    Bruijn, M. P.; van der Linden, A. J.; Ridder, M. L.; van Weers, H. J.

    2016-07-01

    We describe a new fabrication process for a superconducting, flexible, and demountable connector to a kilo-pixel transition edge sensor. The demountable part contains planar coils for inductive coupling, in particular suited for AC-biased frequency domain multiplexed readout. A fixed connection to a chip with superconducting LC filters and SQUID readout is made by gold bump bonding with a connection resistance of 1.1 {× } 10^{-4} Ω . The Nb-based connecting lines on the flexible part show a superconducting transition around 7 K, which enables testing of connectors and LC filters in a simple L-He setup.

  14. Passive cavity surface-emitting lasers: option of temperature-insensitive lasing wavelength for uncooled dense wavelength division multiplexing systems

    NASA Astrophysics Data System (ADS)

    Shchukin, V. A.; Ledentsov, N. N.; Slight, T.; Meredith, W.; Gordeev, N. Y.; Nadtochy, A. M.; Payusov, A. S.; Maximov, M. V.; Blokhin, S. A.; Blokhin, A. A.; Zadiranov, Yu. M.; Maleev, N. A.; Ustinov, V. M.; Choquette, K. D.

    2016-03-01

    A concept of passive cavity surface-emitting laser is proposed aimed to control the temperature shift of the lasing wavelength. The device contains an all-semiconductor bottom distributed Bragg reflector (DBR), in which the active medium is placed, a dielectric resonant cavity and a dielectric top DBR, wherein at least one of the dielectric materials has a negative temperature coefficient of the refractive index, dn/dT < 0. This is shown to be the case for commonly used dielectric systems SiO2/TiO2 and SiO2/Ta2O5. Two SiO2/TiO2 resonant structures having a cavity either of SiO2 or TiO2 were deposited on a substrate, their optical power reflectance spectra were measured at various temperatures, and refractive index temperature coefficients were extracted, dn/dT = 0.0021 K-1 for SiO2 and dn/dT = -0.0092 K-1 for TiO2. Using such dielectric materials allows designing passive cavity surface-emitting lasers having on purpose either positive, or zero, or negative temperature shift of the lasing wavelength dλ/dT. A design for temperature-insensitive lasing wavelength (dλ/dT = 0) is proposed. Employing devices with temperature-insensitive lasing wavelength in wavelength division multiplexing systems may allow significant reducing of the spectral separation between transmission channels and an increase in number of channels for a defined spectral interval enabling low cost energy efficient uncooled devices.

  15. Vector diffraction analysis of optical disk readout.

    PubMed

    Cheng, X; Jia, H; Xu, D

    2000-12-01

    The optical disk readout signals from ROM disks are presented by use of a rigorous three-dimensional vector diffraction method. The optical disk is modeled as a crossed metal grating without restriction on the form of the information marks, and the permittivity of the metal is taken into account. The diffracted field from the disk is obtained by means of decomposing the focused incident beam into a spectrum of plane waves and then calculating the diffracted plane waves for each respective incident component. The readout signal is obtained by integration of the energy-flux density of the diffracted field according to the detection scheme of the optical disk system. A typical digital versatile disk (DVD) system is applied with this theory, and the result is far from that of scalar diffraction theory. PMID:18354657

  16. Single-Readout High-Density Memristor Crossbar

    NASA Astrophysics Data System (ADS)

    Zidan, M. A.; Omran, H.; Naous, R.; Sultan, A.; Fahmy, H. A. H.; Lu, W. D.; Salama, K. N.

    2016-01-01

    High-density memristor-crossbar architecture is a very promising technology for future computing systems. The simplicity of the gateless-crossbar structure is both its principal advantage and the source of undesired sneak-paths of current. This parasitic current could consume an enormous amount of energy and ruin the readout process. We introduce new adaptive-threshold readout techniques that utilize the locality and hierarchy properties of the computer-memory system to address the sneak-paths problem. The proposed methods require a single memory access per pixel for an array readout. Besides, the memristive crossbar consumes an order of magnitude less power than state-of-the-art readout techniques.

  17. Single-Readout High-Density Memristor Crossbar

    PubMed Central

    Zidan, M. A.; Omran, H.; Naous, R.; Sultan, A.; Fahmy, H. A. H.; Lu, W. D.; Salama, K. N.

    2016-01-01

    High-density memristor-crossbar architecture is a very promising technology for future computing systems. The simplicity of the gateless-crossbar structure is both its principal advantage and the source of undesired sneak-paths of current. This parasitic current could consume an enormous amount of energy and ruin the readout process. We introduce new adaptive-threshold readout techniques that utilize the locality and hierarchy properties of the computer-memory system to address the sneak-paths problem. The proposed methods require a single memory access per pixel for an array readout. Besides, the memristive crossbar consumes an order of magnitude less power than state-of-the-art readout techniques. PMID:26738564

  18. Single-Readout High-Density Memristor Crossbar.

    PubMed

    Zidan, M A; Omran, H; Naous, R; Sultan, A; Fahmy, H A H; Lu, W D; Salama, K N

    2016-01-07

    High-density memristor-crossbar architecture is a very promising technology for future computing systems. The simplicity of the gateless-crossbar structure is both its principal advantage and the source of undesired sneak-paths of current. This parasitic current could consume an enormous amount of energy and ruin the readout process. We introduce new adaptive-threshold readout techniques that utilize the locality and hierarchy properties of the computer-memory system to address the sneak-paths problem. The proposed methods require a single memory access per pixel for an array readout. Besides, the memristive crossbar consumes an order of magnitude less power than state-of-the-art readout techniques.

  19. Multiplexing electro-optic architectures for advanced aircraft integrated flight control systems

    NASA Technical Reports Server (NTRS)

    Seal, D. W.

    1989-01-01

    This report describes the results of a 10 month program sponsored by NASA. The objective of this program was to evaluate various optical sensor modulation technologies and to design an optimal Electro-Optic Architecture (EOA) for servicing remote clusters of sensors and actuators in advanced aircraft flight control systems. The EOA's supply optical power to remote sensors and actuators, process the modulated optical signals returned from the sensors, and produce conditioned electrical signals acceptable for use by a digital flight control computer or Vehicle Management System (VMS) computer. This study was part of a multi-year initiative under the Fiber Optic Control System Integration (FOCSI) program to design, develop, and test a totally integrated fiber optic flight/propulsion control system for application to advanced aircraft. Unlike earlier FOCSI studies, this program concentrated on the design of the EOA interface rather than the optical transducer technology itself.

  20. Development of two multiplex PCR systems for the analysis of 14 X-chromosomal STR loci in a southern Brazilian population sample.

    PubMed

    Penna, Larissa Siqueira; Silva, Fernanda Gamio; Salim, Patricia Hartstein; Ewald, Gisele; Jobim, Mariana; Magalhães, José Antônio de Azevedo; Jobim, Luiz Fernando

    2012-03-01

    We developed two multiplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-Multiplex 1 consisted of DXS6807, DXS6800, DXS7424, DXS101, GATA172D05 and HPRTB and X-Multiplex 2 consisted of DXS8378, DXS9898, DXS6801, DXS6809, DXS6789, DXS7133, DXS8377 and DXS7423. In addition, we present allele frequencies for these loci in a south Brazilian population comprising 124 females and 141 males and haplotype frequencies of linked markers for males. Hardy-Weinberg equilibrium (HWE) was tested in the female sample and no significant deviations were found after applying Bonferroni's correction. Linkage disequilibrium (LD) tests were performed for all pairs of loci and three significant results, out of 91 pairwise comparisons, were obtained. We did not find any evidence of linkage disequilibrium between close or linked markers. The power of discrimination in females (PD(F)) varied between 0.832 for DXS6801 and 0.987 for DXS8377. DXS6801 was the least informative marker (PIC = 0.605), while DXS8377 was the most polymorphic (PIC = 0.911), followed by DXS101 (PIC = 0.872). Genetic distances were estimated for each STR marker applying the calculation of F (ST) between our total sample and other studies from Brazil, Europe, Asia and Africa. The most distant populations were Japan, Korea, China, Ghana and Uganda.

  1. Elimination of drift in a fiber-Bragg-grating-based multiplexed Michelson interferometer measurement system

    SciTech Connect

    Ren Junyu; Xie Fang; Chen Zhimin

    2010-02-15

    Random phase drift in single-mode optical fiber interferometers used with measurement systems, which is resulted from various types of environmental disturbances, should be eliminated in order to obtain high measurement precision. We propose an optical fiber interferometric measurement system which has the function of self-eliminating the random phase drift and is stable and robust enough for real-time precision measurement. By employing the characteristics of fiber Bragg gratings, the system interleaves two fiber Michelson interferometers together that share the common-interferometric-optical path. The signal of one of the interferometers is used to stabilize the system while the signal of the other interferometer is used for measurement. An electronic feedback loop for the stabilizing action is designed. The bandwidth of the feedback loop is 5 kHz, sufficiently wide to eliminate random phase drift resulted from various environmental disturbances. The system is endowed with high stability and therefore suitable for real-time precision measurement. By means of an active phase tracking technique to measure displacement, the linear regression coefficient of the displacement measurement results is 0.9998.

  2. Research and development for a free-running readout system for the ATLAS LAr Calorimeters at the high luminosity LHC

    NASA Astrophysics Data System (ADS)

    Hils, Maximilian

    2016-07-01

    The ATLAS Liquid Argon (LAr) Calorimeters were designed and built to measure electromagnetic and hadronic energy in proton-proton collisions produced at the Large Hadron Collider (LHC) at centre-of-mass energies up to 14 TeV and instantaneous luminosities up to 1034 cm-2 s-1. The High Luminosity LHC (HL-LHC) programme is now developed for up to 5-7 times the design luminosity, with the goal of accumulating an integrated luminosity of 3000 fb-1. In the HL-LHC phase, the increased radiation levels and an improved ATLAS trigger system require a replacement of the Front-end (FE) and Back-end (BE) electronics of the LAr Calorimeters. Results from research and development of individual components and their radiation qualification as well as the overall system design will be presented.

  3. Frequency and sensitivity tunable microresonator array for high-speed quantum processor readout

    NASA Astrophysics Data System (ADS)

    Hoskinson, Emile; Whittaker, J. D.; Swenson, L. J.; Volkmann, M. H.; Spear, P.; Altomare, F.; Berkley, A. J.; Bumble, B.; Bunyk, P.; Day, P. K.; Eom, B. H.; Harris, R.; Hilton, J. P.; Johnson, M. W.; Kleinsasser, A.; Ladizinsky, E.; Lanting, T.; Oh, T.; Perminov, I.; Tolkacheva, E.; Yao, J.

    Frequency multiplexed arrays of superconducting microresonators have been used as detectors in a variety of applications. The degree of multiplexing achievable is limited by fabrication variation causing non-uniform shifts in resonator frequencies. We have designed, implemented and characterized a superconducting microresonator readout that incorporates two tunable inductances per detector, allowing independent control of each detector frequency and sensitivity. The tunable inductances are adjusted using on-chip programmable digital-to-analog flux converters, which are programmed with a scalable addressing scheme that requires few external lines.

  4. Breakup of loosely bound nuclei at intermediate energies for nuclear astrophysics and the development of a position sensitive microstrip detector system and its readout electronics using ASICs technologies

    SciTech Connect

    Tribble, Robert E.; Sobotka, Lee G.; Blackmon, Jeff C.; Bertulani, Carlos A.

    2015-12-29

    The work performed under this grant has led to the development of a detection system that will be used to measure reaction rates for proton or neutron capture reactions at stellar energies on radioactive ions far from stability. The reaction rates are needed to better understand the physics of nucleosynthesis in explosive stellar processes such as supernovae and x-ray burst events. The radioactive ions will be produced at the Radioactive Ion Beam Facility (RIBF) at RIKEN near Tokyo, Japan. During the course of this work, the group involved in this project has expanded by several institutions in Europe and Japan and now involves collaborators from the U.S., Japan, Hungary, Romania, Germany, Spain, Italy, China, and South Korea. As part of the project, a novel design based on large-area silicon detectors has been built and tested and the performance characterized in a series of tests using particle beams with a variety of atomic numbers at the Cyclotron Institute of Texas A&M University and the Heavy Ion Medical Accelerator in Chiba facility (HIMAC) in Chiba, Japan. The work has involved mechanical construction of a special purpose vacuum chamber, with a precision mounting system for the silicon detectors, development of a new ASICs readout system that has applications with a wide variety of silicon detector systems, and the development of a data acquisition system that is integrated into the computer system being used at RIBF. The parts noted above that are needed to carry out the research program are completed and ready for installation. Several approved experiments that will use this system will be carried out in the near future. The experimental work has been delayed due to a large increase in the cost and availability of electrical power for RIBF that occurred following the massive earthquake and tsunami that hit Japan in the spring of 2011. Another component of the research carried out with this grant involved developing the theoretical tools that are required

  5. [Respiratory viral diagnosis by using an automated system of multiplex PCR (FilmArray) compared to conventional methods].

    PubMed

    Marcone, Débora N; Carballal, Guadalupe; Ricarte, Carmen; Echavarria, Marcela

    2015-01-01

    Acute respiratory infections, which are commonly caused by viruses, are an important cause of morbidity and mortality in children. In Argentina, national surveillance programs for the detection of respiratory viruses are usually performed by using immunofluorescence (IF) assays, although it is well known that molecular methods are more sensitive. An automated multiplex PCR device, the FilmArray-Respiratory Panel (FilmArray-RP), can detect 17 viral and 3 bacterial pathogens in a closed system that requires only 5 min of hands-on time and 1h of instrumentation time. A total of 315 respiratory samples from children under 6 years of age suffering from acute respiratory infections, were studied by IF for 8 respiratory viruses and by RT-PCR for rhinoviruses. Later, these samples were tested by the FilmArray-RP. The positivity frequency obtained for the 9 viruses tested was 75% by IF/RT-PCR and 92% by the FilmArray-RP. The positive and negative percent agreement between both methods was 70.5% whereas the negative percent agreement was 99.6% (95% confidence interval:65.5-75.1 and 99.2-99.8 respectively). The FilmArray-RP allowed a higher positive diagnosis (97%) and detected other viruses such as coronavirus NL63, 229E, OC43, HKU1 (10%) and bocavirus (18%). In addition, this method identified multiple coinfections (39%) with 2, 3, 4 and up to 5 different viruses. At present, IF is still the most frequently used method in most Latin American countries for respiratory viruses diagnosis due to its low cost, its capability to process a high number of samples simultaneously and the fast determination of results for the most frequent viruses, which are available within 5h. However, the coming incorporation of molecular methods in routine procedures will significantly increase the diagnostic yield of these infections.

  6. Digital Frequency Domain Multiplexer for mm-Wavelength Telescopes

    SciTech Connect

    Spieler, Helmuth G; Dobbs, Matt; Bissonnette, Eric; Spieler, Helmuth G.

    2007-07-23

    An FPGA based digital signal processing (DSP) system for biasing and reading out multiplexed bolometric detectors for mm-wavelength telescopes is presented. This readout system is being deployed for balloon-borne and ground based cosmology experiments with the primary goal of measuring the signature of inflation with the Cosmic Microwave Background Radiation. The system consists of analog superconducting electronics running at 250 mK and 4 K, coupled to digital room temperature backend electronics described here. The digital electronics perform the real time functionality with DSP algorithms implemented in firmware. A soft embedded processor provides all of the slow housekeeping control and communications. Each board in the system synthesizes multi-frequency combs of 8 to 32 carriers in the MHz band to bias the detectors. After the carriers have been modulated with the sky-signal by the detectors, the same boards digitize the comb directly. The carriers are mixed down to base-band and low pass filtered. The signal bandwidth of 0.050Hz-100 Hz places extreme requirements on stability and requires powerful filtering techniques to recover the sky-signal from the MHz carriers.

  7. Development and evaluation of an optimized multiplex array detector Raman system for field environmental analysis

    SciTech Connect

    Denton, M.B.; Gilmore, D.A.

    1995-12-31

    An optimized, compact Raman system for field environmental analysis is being developed. The Raman system uses diode laser excitation, fiber-optic probes, a new generations of imaging spectrometers, and solid state detectors. Emphasis has centered on optimizing the Raman apparatus, determining the best fiber optic probe technology that will allow remote sensing (i.e. down a well or simultaneous multipoint monitoring), establishing quality control procedures, and developing camera control and data analysis software. Sensitivities for a variety of compounds will be presented demonstrating major reductions in detection limits. New approaches for implementing very high throughput monochromators will be discussed, and their impact on the overall Raman system considered. The latest generation of solid state detectors including Charged-Coupled Devices (CCD) and Charge-Injection Devices (CID) will be reviewed. Over the last several years, these detectors have contributed to major advances in Raman spectroscopy. An overview of combining proper optical systems with currently available and soon to be introduced detectors will be presented.

  8. VCSELs for interferometric readout of MEMS sensors

    NASA Astrophysics Data System (ADS)

    Serkland, Darwin K.; Geib, Kent M.; Peake, Gregory M.; Keeler, Gordon A.; Shaw, Michael J.; Baker, Michael S.; Okandan, Murat

    2016-03-01

    We report on the development of single-frequency VCSELs (vertical-cavity surface-emitting lasers) for sensing the position of a moving MEMS (micro-electro-mechanical system) object with resolution much less than 1nm. Position measurement is the basis of many different types of MEMS sensors, including accelerometers, gyroscopes, and pressure sensors. Typically, by switching from a traditional capacitive electronic readout to an interferometric optical readout, the resolution can be improved by an order of magnitude with a corresponding improvement in MEMS sensor performance. Because the VCSEL wavelength determines the scale of the position measurement, laser wavelength (frequency) stability is desirable. This paper discusses the impact of VCSEL amplitude and frequency noise on the position measurement.

  9. The fine grained detector readout electronics

    NASA Astrophysics Data System (ADS)

    Retière, F.; T2K/FGD Collaboration

    2010-11-01

    The Fine Grained Detector (FGD) readout electronics was designed to record a snapshot of the detector activity before, during and after the neutrino beam spill produced by the J-PARC accelerator complex in Tokai, Japan. The FGD is a key element of T2K near detector currently being constructed. It will act as an active target detecting charged particles produced within its scintillator bars. The original feature of the FGD electronics is to readout Multi-Pixel Photon Counters by recording waveforms using Switch Capacitor Array chips (AFTER ASIC) designed for T2K Time Projection Chamber. In these proceedings, we describe the architecture of the FGD electronics. Beam results show that the physics requirements are met or exceeded. The relatively slow sampling frequency of the AFTER ASIC does not impair the timing resolution significantly. In addition, the very good efficiency of the system for detecting Michel electrons produced by pions or muons decay has been demonstrated.

  10. Working model of the London moment readout system. [the study of super fluid plug operation using liquid helium

    NASA Technical Reports Server (NTRS)

    Hendricks, J. B.; Karr, G. R.

    1975-01-01

    The operating characteristics of a porous plug which has liquid helium on one side and which is pumped on under vacuum on the other side are discussed. The system investigated consists of a container of liquid helium which is well isolated, and the only means for mass flow out of the container is through a plug mode of porous material. The plug was assumed to have liquid helium on the container side while the other side of the plug is evacuated. Three cases were considered: (1) perfect evacuation with zero pressure, (2) evacuation through a chocked orifice, and (3) evacuation through a long, small diameter pipe with heating. Mass flow rates were determined along with mass flow at temperature above the lambda point temperature. Solutions were obtained for normal and superfluid velocity.

  11. Multiplexing a high-throughput liability assay to leverage efficiencies.

    PubMed

    Herbst, John; Anthony, Monique; Stewart, Jeremy; Connors, David; Chen, Taosheng; Banks, Martyn; Petrillo, Edward W; Agler, Michele

    2009-06-01

    In order to identify potential cytochrome P-450 3A4 (drug-metabolizing enzyme) inducers at an early stage of the drug discovery process, a cell-based transactivation high-throughput luciferase reporter assay for the human pregnane X receptor (PXR) in HepG2 cells has been implemented and multiplexed with a viability end point for data interpretation, as part of a Lead Profiling portfolio of assays. As a routine part of Lead Profiling operations, assays are periodically evaluated for utility as well as for potential improvements in technology or process. We used a recent evaluation of our PXR-transactivation assay as a model for the application of Lean Thinking-based process analysis to lab-bench assay optimization and automation. This resulted in the development of a 384-well multiplexed homogeneous assay simultaneously detecting PXR transactivation and HepG2 cell cytotoxicity. In order to multiplex fluorescent and luminescent read-outs, modifications to each assay were necessary, which included optimization of multiple assay parameters such as cell density, plate type, and reagent concentrations. Subsequently, a set of compounds including known cytotoxic compounds and PXR inducers were used to validate the multiplexed assay. Results from the multiplexed assay correlate well with those from the singleplexed assay formats measuring PXR transactivation and viability separately. Implementation of the multiplexed assay for routine compound profiling provides improved data quality, sample conservation, cost savings, and resource efficiencies.

  12. A cryo-amplifier working in a double loop-flux locked loop scheme for SQUID readout of TES detectors

    NASA Astrophysics Data System (ADS)

    Torrioli, Guido; Bastia, Paolo; Piro, Luigi; Macculi, Claudio; Colasanti, Luca

    2010-07-01

    In this paper we report on a novel SQUID readout scheme, called Double Loop-Flux Locked loop (DL-FLL), that we are investigating in the frame of ASI and ESA technological development contracts. This scheme is based on the realization of a cryogenic amplifier which is used in order to readout TES detectors in the Frequency Division Multiplexing technique, where high loop-gain is required up to few MHz. Loop-gain in feedback systems is, usually, limited by the propagation delay of the signals traveling in the loop because of the distance between the feedback loop elements. This problem is particularly evident in the case of SQUID systems, where the elements of the feedback loop are placed both at cryogenic and room temperature. To solve this issue we propose a low power dissipation cryo-amplifier capable to work at cryogenic temperatures so that it can be placed close to the SQUID realizing a local cryogenic loop. The adoption of the DL-FLL scheme allows to simplify considerably the cryo-amplifier which, being AC-coupled, don't require the features of a precision DC-coupled amplifier and can be made with a limited number of electronic components and with a consequent reduction of power dissipation.

  13. Patterned Plasmonic Nanoparticle Arrays for Microfluidic and Multiplexed Biological Assays.

    PubMed

    He, Jie; Boegli, Michelle; Bruzas, Ian; Lum, William; Sagle, Laura

    2015-11-17

    For applications ranging from medical diagnostics and drug screening to chemical and biological warfare detection, inexpensive, rapid-readout, portable devices are required. Localized surface plasmon resonance (LSPR) technologies show substantial promise toward meeting these goals, but the generation of portable, multiplexed and/or microfluidic devices incorporating sensitive nanoparticle arrays is only in its infancy. Herein, we have combined photolithography with Hole Mask Colloidal lithography to pattern uniform nanoparticle arrays for both microfluidic and multiplexed devices. The first proof-of-concept study is carried out with 5- and 7-channel microfluidic devices to acquire one-shot binding curves and protein binding kinetic data. The second proof-of-concept study involved the fabrication of a 96-spot plate that can be inserted into a standard plate reader for the multiplexed detection of protein binding. This versatile fabrication technique should prove useful in next generation chips for bioassays and genetic screening. PMID:26494412

  14. Analysis of position error by time constant in read-out resistive network for gamma-ray imaging detection system

    NASA Astrophysics Data System (ADS)

    Jeon, Su-Jin; Park, Chang-In; Son, Byung-Hee; Jung, Mi; Jang, Teak-Jin; Lee, Chun-Sik; Choi, Young-Wan

    2016-03-01

    Position-sensitive photomultiplier tubes (PSPMTs) in array are used as gamma ray position detector. Each PMT converts the light of wide spectrum range (100 nm ~ 2500 nm) to electrical signal with amplification. Because detection system size is determined by the number of output channels in the PSPMTs, resistive network has been used for reducing the number of output channels. The photo-generated current is distributed to the four output current pulses according to a ratio by resistance values of resistive network. The detected positions are estimated by the peak value of the distributed current pulses. However, due to parasitic capacitance of PSPMTs in parallel with resistor in the resistive network, the time constants should be considered. When the duration of current pulse is not long enough, peak value of distributed pulses is reduced and detected position error is increased. In this paper, we analyzed the detected position error in the resistive network and variation of time constant according to the input position of the PSPMTs.

  15. Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    NASA Astrophysics Data System (ADS)

    Lee, Jung-Rok; Haddon, D. James; Wand, Hannah E.; Price, Jordan V.; Diep, Vivian K.; Hall, Drew A.; Petri, Michelle; Baechler, Emily C.; Balboni, Imelda M.; Utz, Paul J.; Wang, Shan X.

    2016-06-01

    High titer, class-switched autoantibodies are a hallmark of systemic lupus erythematosus (SLE). Dysregulation of the interferon (IFN) pathway is observed in individuals with active SLE, although the association of specific autoantibodies with chemokine score, a combined measurement of three IFN-regulated chemokines, is not known. To identify autoantibodies associated with chemokine score, we developed giant magnetoresistive (GMR) biosensor microarrays, which allow the parallel measurement of multiple serum antibodies to autoantigens and peptides. We used the microarrays to analyze serum samples from SLE patients and found individuals with high chemokine scores had significantly greater reactivity to 13 autoantigens than individuals with low chemokine scores. Our findings demonstrate that multiple autoantibodies, including antibodies to U1-70K and modified histone H2B tails, are associated with IFN dysregulation in SLE. Further, they show the microarrays are capable of identifying autoantibodies associated with relevant clinical manifestations of SLE, with potential for use as biomarkers in clinical practice.

  16. Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    PubMed Central

    Lee, Jung-Rok; Haddon, D. James; Wand, Hannah E.; Price, Jordan V.; Diep, Vivian K.; Hall, Drew A.; Petri, Michelle; Baechler, Emily C.; Balboni, Imelda M.; Utz, Paul J.; Wang, Shan X.

    2016-01-01

    High titer, class-switched autoantibodies are a hallmark of systemic lupus erythematosus (SLE). Dysregulation of the interferon (IFN) pathway is observed in individuals with active SLE, although the association of specific autoantibodies with chemokine score, a combined measurement of three IFN-regulated chemokines, is not known. To identify autoantibodies associated with chemokine score, we developed giant magnetoresistive (GMR) biosensor microarrays, which allow the parallel measurement of multiple serum antibodies to autoantigens and peptides. We used the microarrays to analyze serum samples from SLE patients and found individuals with high chemokine scores had significantly greater reactivity to 13 autoantigens than individuals with low chemokine scores. Our findings demonstrate that multiple autoantibodies, including antibodies to U1-70K and modified histone H2B tails, are associated with IFN dysregulation in SLE. Further, they show the microarrays are capable of identifying autoantibodies associated with relevant clinical manifestations of SLE, with potential for use as biomarkers in clinical practice. PMID:27279139

  17. Monitoring of Time-Dependent System Profiles by Multiplex Gas Chromatography with Maximum Entropy Demodulation

    NASA Technical Reports Server (NTRS)

    Becker, Joseph F.; Valentin, Jose

    1996-01-01

    The maximum entropy technique was successfully applied to the deconvolution of overlapped chromatographic peaks. An algorithm was written in which the chromatogram was represented as a vector of sample concentrations multiplied by a peak shape matrix. Simulation results demonstrated that there is a trade off between the detector noise and peak resolution in the sense that an increase of the noise level reduced the peak separation that could be recovered by the maximum entropy method. Real data originated from a sample storage column was also deconvoluted using maximum entropy. Deconvolution is useful in this type of system because the conservation of time dependent profiles depends on the band spreading processes in the chromatographic column, which might smooth out the finer details in the concentration profile. The method was also applied to the deconvolution of previously interpretted Pioneer Venus chromatograms. It was found in this case that the correct choice of peak shape function was critical to the sensitivity of maximum entropy in the reconstruction of these chromatograms.

  18. A fast and reliable readout method for quantitative analysis of surface-enhanced Raman scattering nanoprobes on chip surface

    NASA Astrophysics Data System (ADS)

    Chang, Hyejin; Kang, Homan; Jeong, Sinyoung; Ko, Eunbyeol; Lee, Yoon-Sik; Lee, Ho-Young; Jeong, Dae Hong

    2015-05-01

    Surface-enhanced Raman scattering techniques have been widely used for bioanalysis due to its high sensitivity and multiplex capacity. However, the point-scanning method using a micro-Raman system, which is the most common method in the literature, has a disadvantage of extremely long measurement time for on-chip immunoassay adopting a large chip area of approximately 1-mm scale and confocal beam point of ca. 1-μm size. Alternative methods such as sampled spot scan with high confocality and large-area scan method with enlarged field of view and low confocality have been utilized in order to minimize the measurement time practically. In this study, we analyzed the two methods in respect of signal-to-noise ratio and sampling-led signal fluctuations to obtain insights into a fast and reliable readout strategy. On this basis, we proposed a methodology for fast and reliable quantitative measurement of the whole chip area. The proposed method adopted a raster scan covering a full area of 100 μm × 100 μm region as a proof-of-concept experiment while accumulating signals in the CCD detector for single spectrum per frame. One single scan with 10 s over 100 μm × 100 μm area yielded much higher sensitivity compared to sampled spot scanning measurements and no signal fluctuations attributed to sampled spot scan. This readout method is able to serve as one of key technologies that will bring quantitative multiplexed detection and analysis into practice.

  19. A fast and reliable readout method for quantitative analysis of surface-enhanced Raman scattering nanoprobes on chip surface

    SciTech Connect

    Chang, Hyejin; Jeong, Sinyoung; Ko, Eunbyeol; Jeong, Dae Hong E-mail: debobkr@gmail.com; Kang, Homan; Lee, Yoon-Sik E-mail: debobkr@gmail.com; Lee, Ho-Young E-mail: debobkr@gmail.com

    2015-05-15

    Surface-enhanced Raman scattering techniques have been widely used for bioanalysis due to its high sensitivity and multiplex capacity. However, the point-scanning method using a micro-Raman system, which is the most common method in the literature, has a disadvantage of extremely long measurement time for on-chip immunoassay adopting a large chip area of approximately 1-mm scale and confocal beam point of ca. 1-μm size. Alternative methods such as sampled spot scan with high confocality and large-area scan method with enlarged field of view and low confocality have been utilized in order to minimize the measurement time practically. In this study, we analyzed the two methods in respect of signal-to-noise ratio and sampling-led signal fluctuations to obtain insights into a fast and reliable readout strategy. On this basis, we proposed a methodology for fast and reliable quantitative measurement of the whole chip area. The proposed method adopted a raster scan covering a full area of 100 μm × 100 μm region as a proof-of-concept experiment while accumulating signals in the CCD detector for single spectrum per frame. One single scan with 10 s over 100 μm × 100 μm area yielded much higher sensitivity compared to sampled spot scanning measurements and no signal fluctuations attributed to sampled spot scan. This readout method is able to serve as one of key technologies that will bring quantitative multiplexed detection and analysis into practice.

  20. Structure of triadic relations in multiplex networks

    NASA Astrophysics Data System (ADS)

    Cozzo, Emanuele; Kivelä, Mikko; De Domenico, Manlio; Solé-Ribalta, Albert; Arenas, Alex; Gómez, Sergio; Porter, Mason A.; Moreno, Yamir

    2015-07-01

    Recent advances in the study of networked systems have highlighted that our interconnected world is composed of networks that are coupled to each other through different ‘layers’ that each represent one of many possible subsystems or types of interactions. Nevertheless, it is traditional to aggregate multilayer networks into a single weighted network in order to take advantage of existing tools. This is admittedly convenient, but it is also extremely problematic, as important information can be lost as a result. It is therefore important to develop multilayer generalizations of network concepts. In this paper, we analyze triadic relations and generalize the idea of transitivity to multiplex networks. By focusing on triadic relations, which yield the simplest type of transitivity, we generalize the concept and computation of clustering coefficients to multiplex networks. We show how the layered structure of such networks introduces a new degree of freedom that has a fundamental effect on transitivity. We compute multiplex clustering coefficients for several real multiplex networks and illustrate why one must take great care when generalizing standard network concepts to multiplex networks. We also derive analytical expressions for our clustering coefficients for ensemble averages of networks in a family of random multiplex networks. Our analysis illustrates that social networks have a strong tendency to promote redundancy by closing triads at every layer and that they thereby have a different type of multiplex transitivity from transportation networks, which do not exhibit such a tendency. These insights are invisible if one only studies aggregated networks.

  1. Design and performance of the new cathode readout proportional chambers in LASS

    SciTech Connect

    Aiken, G.; Aston, D.; Dunwoodie, W.

    1980-10-01

    The design and construction of a new proportional chamber system for the LASS spectrometer are discussed. This system consists of planar and cylindrical chambers employing anode wire and cathode strip readout techniques. The good timing characteristics of anode readout combine with the excellent spatial resolution of cathode readout to provide powerful and compact detectors. Preliminary resolution data are presented along with operating characteristics of the various devices.

  2. XAMPS Detectors Readout ASIC for LCLS

    SciTech Connect

    Dragone, A; Pratte, J.F.; Rehak, P.; Carini, G.A.; Herbst, R.; O'Connor, P.; Siddons, D.P.; /BNL, NSLS

    2008-12-18

    An ASIC for the readout of signals from X-ray Active Matrix Pixel Sensor (XAMPS) detectors to be used at the Linac Coherent Light Source (LCLS) is presented. The X-ray Pump Probe (XPP) instrument, for which the ASIC has been designed, requires a large input dynamic range on the order of 104 photons at 8 keV with a resolution of half a photon FWHM. Due to the size of the pixel and the length of the readout line, large input capacitance is expected, leading to stringent requirement on the noise optimization. Furthermore, the large number of pixels needed for a good position resolution and the fixed LCLS beam period impose limitations on the time available for the single pixel readout. Considering the periodic nature of the LCLS beam, the ASIC developed for this application is a time-variant system providing low-noise charge integration, filtering and correlated double sampling. In order to cope with the large input dynamic range a charge pump scheme implementing a zero-balance measurement method has been introduced. It provides an on chip 3-bit coarse digital conversion of the integrated charge. The residual charge is sampled using correlated double sampling into analog memory and measured with the required resolution. The first 64 channel prototype of the ASIC has been fabricated in TSMC CMOS 0.25 {micro}m technology. In this paper, the ASIC architecture and performances are presented.

  3. EMIR high-dynamic range readout modes

    NASA Astrophysics Data System (ADS)

    Nuñez, Miguel; Gago, Fernando; Garzón, Francisco; Díaz, José J.; Barreto, Mary; Patrón, Jesús; González-Fenández, Carlos; Hammersley, Peter L.; López, Luis; Castro, Nieves

    2012-07-01

    EMIR is the NIR imager and multiobject spectrograph being built as a common user instrument for the GTC and it is currently entering in the integration and verification phase at system level. EMIR is being built by a Consortium of Spanish and French institutes led by the IAC. In this paper we describe the readout modes of EMIR detector, a Hawaii2 FPA, after two full calibrations campaigns. Besides the standard set of modes (reset-read, CDS, Fowler, Follow-up the ramp), the modified SDSU-III hardware and home made software will also offer high dynamic range readout modes, which will improve the ability of the instrument to sound densely populated areas which often are made of objects with large differences in brightness. These new high dynamic range modes are: single readout with very short integration time, window mode and combination of both. The results show that the new modes behave linearly with different exposition times, improve the maximum frame rate and increase the saturation limit in image mode for EMIR instrument.

  4. A Multiplex Snapback Primer System for the Enrichment and Detection of JAK2 V617F and MPL W515L/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms

    PubMed Central

    Zhang, Yunqing; Zhang, Xinju; Xu, Xiao; Kang, Zhihua; Li, Shibao; Zhang, Chen; Su, Bing

    2014-01-01

    A multiplex snapback primer system was developed for the simultaneous detection of JAK2 V617F and MPL W515L/K mutations in Philadelphia chromosome- (Ph-) negative myeloproliferative neoplasms (MPNs). The multiplex system comprises two snapback versus limiting primer sets for JAK2 and MPL mutation enrichment and detection, respectively. Linear-After exponential (LATE) PCR strategy was employed for the primer design to maximize the amplification efficiency of the system. Low ionic strength buffer and rapid PCR protocol allowed for selective amplification of the mutant alleles. Amplification products were analyzed by melting curve analysis for mutation identification. The multiplex system archived 0.1% mutation load sensitivity and <5% coefficient of variation inter-/intra-assay reproducibility. 120 clinical samples were tested by the multiplex snapback primer assay, and verified with amplification refractory system (ARMS), quantitative PCR (qPCR) and Sanger sequencing method. The multiplex system, with a favored versatility, provided the molecular diagnosis of Ph-negative MPNs with a suitable implement and simplified the genetic test process. PMID:24729973

  5. Retroreflective systems for remote readout

    DOEpatents

    Deason, Vance A.; Colwell, Frederick S.; Ricks, Kirk L.

    1998-01-01

    A sensing device for sensing an environmental factor. The device includes a retroreflective layer disposed in a parallel, facing relationship with a sensing layer. The sensing layer has an initial optical absorption capacity for (i) sensing a presence of an environmental factor, (ii) experiencing a change in optical absorption capacity responsive to said environmental factor, and (iii) transmitting and attenuating light. A first portion of the sensing layer is sealed off from exposure to the environment while a second portion remains exposed to the environment such that, when the environmental factor is present, the first portion of the sensing layer is prevented from experiencing a change in optical absorption capacity responsive to said environmental factor. Well-collimated light beams are passed through the sensing layer and are reflected back from the retroreflective layer for processing. When the environmental factor is present, the beams which pass through the second portion are attenuated responsive to an increase in optical absorption capacity and are compared with the non-attenuated beams passing through the first portion to calculate the presence and quantity of the environmental factor.

  6. Retroreflective systems for remote readout

    DOEpatents

    Deason, V.A.; Colwell, F.S.; Ricks, K.L.

    1998-10-13

    A sensing device is described for sensing an environmental factor. The device includes a retroreflective layer disposed in a parallel, facing relationship with a sensing layer. The sensing layer has an initial optical absorption capacity for (1) sensing a presence of an environmental factor, (2) experiencing a change in optical absorption capacity responsive to said environmental factor, and (3) transmitting and attenuating light. A first portion of the sensing layer is sealed off from exposure to the environment while a second portion remains exposed to the environment such that, when the environmental factor is present, the first portion of the sensing layer is prevented from experiencing a change in optical absorption capacity responsive to said environmental factor. Well-collimated light beams are passed through the sensing layer and are reflected back from the retroreflective layer for processing. When the environmental factor is present, the beams which pass through the second portion are attenuated responsive to an increase in optical absorption capacity and are compared with the non-attenuated beams passing through the first portion to calculate the presence and quantity of the environmental factor. 7 figs.

  7. Communicability reveals a transition to coordinated behavior in multiplex networks

    NASA Astrophysics Data System (ADS)

    Estrada, Ernesto; Gómez-Gardeñes, Jesús

    2014-04-01

    We analyze the flow of information in multiplex networks by means of the communicability function. First, we generalize this measure from its definition from simple graphs to multiplex networks. Then, we study its relevance for the analysis of real-world systems by studying a social multiplex where information flows using formal-informal channels and an air transportation system where the layers represent different air companies. Accordingly, the communicability, which is essential for the good performance of these complex systems, emerges at a systemic operation point in the multiplex where the performance of the layers operates in a coordinated way very differently from the state represented by a collection of unconnected networks.

  8. Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay.

    PubMed

    Kim, Suk Ran; Ki, Chang-Seok; Lee, Nam Yong

    2009-03-01

    Acute viral respiratory infections are among the most common causes of human disease. Rapid and accurate diagnosis of viral respiratory infections is important for providing timely therapeutic interventions. This study evaluated a new multiplex PCR assay (Seegene Inc., Seoul, Korea) for simultaneous detection and identification of 12 respiratory viruses using two primer mixes. The viruses included parainfluenza viruses 1, 2, and 3, human metapneumovirus, human coronavirus 229E/NL63 and OC43, adenovirus, influenza viruses A and B, human respiratory syncytial viruses A and B, and human rhinovirus A. The analytical sensitivity of the assay was 10-100 copies per reaction for each type of virus. There was no cross-reactivity with common bacterial or viral pathogens. A comparison with conventional viral culture and immunofluorescence was carried out using 101 respiratory specimens from 92 patients. Using viral culture, 57 specimens (56.4%) were positive without co-infection. The same viruses were identified in all 57 specimens using the multiplex PCR. Seven of the 57 specimens (12.3%) were found to be co-infected with other respiratory viruses, and 19 of 44 (43.2%) specimens which were negative by culture were positive by the multiplex PCR. The Seeplex Respiratory Virus Detection assay represents a significant improvement over the conventional methods for the detection of a broad spectrum of respiratory viruses.

  9. Intracavity absorption multiplexed sensor network based on dense wavelength division multiplexing filter.

    PubMed

    Zhang, Haiwei; Lu, Ying; Duan, Liangcheng; Zhao, Zhiqiang; Shi, Wei; Yao, Jianquan

    2014-10-01

    We report the system design and experimental verification of an intracavity absorption multiplexed sensor network with hollow core photonic crystal fiber (HCPCF) sensors and dense wavelength division multiplexing (DWDM) filters. Compared with fiber Bragg grating (FBG), it is easier for the DWDM to accomplish a stable output. We realize the concentration detection of three gas cells filled with acetylene. The sensitivity is up to 100 ppmV at 1536.71 nm. Voltage gradient is firstly used to optimize the intracavity sensor network enhancing the detection efficiency up to 6.5 times. To the best of our knowledge, DWDM is firstly used as a wavelength division multiplexing device to realize intracavity absorption multiplexed sensor network. It make it possible to realize high capacity intracavity sensor network via multiplexed technique. PMID:25322029

  10. Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis

    PubMed Central

    Zhang, Yanmei; Zhao, Fuju; Kong, Mimi; Wang, Shiwen; Nan, Li; Hu, Binjie; Olszewski, Michal A.; Miao, Yingxin; Ji, Danian; Jiang, Wenrong; Fang, Yi; Zhang, Jinghao; Chen, Fei; Xiang, Ping; Wu, Yong

    2016-01-01

    Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. Existing detection methods are low-throughput, time-consuming and labor intensive. Therefore, a rapid and high-throughput method is needed for clinical diagnosis, treatment, and monitoring for H. pylori. High-throughput Multiplex Genetic Detection System (HMGS) assay was established to simultaneously detect and analyze a set of genes for H. pylori identification, quantification, virulence, and drug resistance by optimizing the singlet-PCR and multiple primers assay. Twenty-one pairs of chimeric primers consisted of conserved and specific gene sequences of H. pylori tagged with universal sequence at the 5′ end were designed. Singlet-PCR assay and multiple primers assay were developed to optimize the HMGS. The specificity of HMGS assay was evaluated using standard H. pylori strains and bacterial controls. Six clinical isolates with known genetic background of target genes were detected to assess the accuracy of HMGS assay. Artificial mixed pathogen DNA templates were used to evaluate the ability to distinguish mixed infections using HMGS assay. Furthermore, gastric biopsy specimens with corresponding isolated strains were used to assess the capability of HMGS assay in detecting biopsy specimens directly. HMGS assay was specific for H. pylori identification. HMGS assay for H. pylori target genes detection were completely consistent with the corresponding genetic background. Mixed infection with different drug-resistant isolates of H. pylori could be distinguished by HMGS assay. HMGS assay could efficiently diagnose H. pylori infection in gastric biopsy specimens directly. HMGS assay is a rapid and high throughput method for the simultaneous identification and quantification of H. pylori, analysis of

  11. Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis

    PubMed Central

    Zhang, Yanmei; Zhao, Fuju; Kong, Mimi; Wang, Shiwen; Nan, Li; Hu, Binjie; Olszewski, Michal A.; Miao, Yingxin; Ji, Danian; Jiang, Wenrong; Fang, Yi; Zhang, Jinghao; Chen, Fei; Xiang, Ping; Wu, Yong

    2016-01-01

    Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. Existing detection methods are low-throughput, time-consuming and labor intensive. Therefore, a rapid and high-throughput method is needed for clinical diagnosis, treatment, and monitoring for H. pylori. High-throughput Multiplex Genetic Detection System (HMGS) assay was established to simultaneously detect and analyze a set of genes for H. pylori identification, quantification, virulence, and drug resistance by optimizing the singlet-PCR and multiple primers assay. Twenty-one pairs of chimeric primers consisted of conserved and specific gene sequences of H. pylori tagged with universal sequence at the 5′ end were designed. Singlet-PCR assay and multiple primers assay were developed to optimize the HMGS. The specificity of HMGS assay was evaluated using standard H. pylori strains and bacterial controls. Six clinical isolates with known genetic background of target genes were detected to assess the accuracy of HMGS assay. Artificial mixed pathogen DNA templates were used to evaluate the ability to distinguish mixed infections using HMGS assay. Furthermore, gastric biopsy specimens with corresponding isolated strains were used to assess the capability of HMGS assay in detecting biopsy specimens directly. HMGS assay was specific for H. pylori identification. HMGS assay for H. pylori target genes detection were completely consistent with the corresponding genetic background. Mixed infection with different drug-resistant isolates of H. pylori could be distinguished by HMGS assay. HMGS assay could efficiently diagnose H. pylori infection in gastric biopsy specimens directly. HMGS assay is a rapid and high throughput method for the simultaneous identification and quantification of H. pylori, analysis of

  12. Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis.

    PubMed

    Zhang, Yanmei; Zhao, Fuju; Kong, Mimi; Wang, Shiwen; Nan, Li; Hu, Binjie; Olszewski, Michal A; Miao, Yingxin; Ji, Danian; Jiang, Wenrong; Fang, Yi; Zhang, Jinghao; Chen, Fei; Xiang, Ping; Wu, Yong; Zhao, Hu

    2016-01-01

    Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. Existing detection methods are low-throughput, time-consuming and labor intensive. Therefore, a rapid and high-throughput method is needed for clinical diagnosis, treatment, and monitoring for H. pylori. High-throughput Multiplex Genetic Detection System (HMGS) assay was established to simultaneously detect and analyze a set of genes for H. pylori identification, quantification, virulence, and drug resistance by optimizing the singlet-PCR and multiple primers assay. Twenty-one pairs of chimeric primers consisted of conserved and specific gene sequences of H. pylori tagged with universal sequence at the 5' end were designed. Singlet-PCR assay and multiple primers assay were developed to optimize the HMGS. The specificity of HMGS assay was evaluated using standard H. pylori strains and bacterial controls. Six clinical isolates with known genetic background of target genes were detected to assess the accuracy of HMGS assay. Artificial mixed pathogen DNA templates were used to evaluate the ability to distinguish mixed infections using HMGS assay. Furthermore, gastric biopsy specimens with corresponding isolated strains were used to assess the capability of HMGS assay in detecting biopsy specimens directly. HMGS assay was specific for H. pylori identification. HMGS assay for H. pylori target genes detection were completely consistent with the corresponding genetic background. Mixed infection with different drug-resistant isolates of H. pylori could be distinguished by HMGS assay. HMGS assay could efficiently diagnose H. pylori infection in gastric biopsy specimens directly. HMGS assay is a rapid and high throughput method for the simultaneous identification and quantification of H. pylori, analysis of

  13. Simultaneous measurement of dynamic displacement and strain in a single fiber using coarse wavelength-division multiplexing and fiber Bragg-grating filter-based sensing system.

    PubMed

    Chuang, Kuo-Chih; Ma, Chien-Ching; Wang, Hwa-Chun

    2016-03-20

    Displacement and strain, two of the most important physical quantities in experimental solid mechanics, are seldomly measured simultaneously in a single experimental configuration. In order to provide and improve corresponding sensing techniques, an experimental setup system for simultaneous measurement of dynamic displacement and strain on a flexible cantilever beam using two fiber Bragg gratings (FBGs) in a single fiber is proposed. To realize high-speed multiplexing and demodulation, a configuration incorporating a coarse wavelength-division multiplexing (CWDM) technique and an FBG transmission filter is implemented. The cantilever beam is subjected to steel-ball impact from which the dynamic multipoint displacement/strain sensing performances of the CWDM and FBG filter-based sensing system are demonstrated. Experimental results in temporal and frequency domain are compared with those obtained by the finite element method (FEM) predictions based on identification of the impact-loading history. A noncontact Fotonic displacement sensor and a polyvinylidene-fluoride film (PVDF) strain sensor are also used for comparison. With transient and resonant frequency simulations conducted by the FEM, loading effects of the sensing system are examined. The results obtained in this study indicate that the proposed CWDM and FBG filter-based sensing system is capable of performing simultaneous multipoint displacement/strain measurements in a single fiber with large bandwidth, high sensitivity, and low intensity loss. PMID:27140584

  14. Simultaneous measurement of dynamic displacement and strain in a single fiber using coarse wavelength-division multiplexing and fiber Bragg-grating filter-based sensing system.

    PubMed

    Chuang, Kuo-Chih; Ma, Chien-Ching; Wang, Hwa-Chun

    2016-03-20

    Displacement and strain, two of the most important physical quantities in experimental solid mechanics, are seldomly measured simultaneously in a single experimental configuration. In order to provide and improve corresponding sensing techniques, an experimental setup system for simultaneous measurement of dynamic displacement and strain on a flexible cantilever beam using two fiber Bragg gratings (FBGs) in a single fiber is proposed. To realize high-speed multiplexing and demodulation, a configuration incorporating a coarse wavelength-division multiplexing (CWDM) technique and an FBG transmission filter is implemented. The cantilever beam is subjected to steel-ball impact from which the dynamic multipoint displacement/strain sensing performances of the CWDM and FBG filter-based sensing system are demonstrated. Experimental results in temporal and frequency domain are compared with those obtained by the finite element method (FEM) predictions based on identification of the impact-loading history. A noncontact Fotonic displacement sensor and a polyvinylidene-fluoride film (PVDF) strain sensor are also used for comparison. With transient and resonant frequency simulations conducted by the FEM, loading effects of the sensing system are examined. The results obtained in this study indicate that the proposed CWDM and FBG filter-based sensing system is capable of performing simultaneous multipoint displacement/strain measurements in a single fiber with large bandwidth, high sensitivity, and low intensity loss.

  15. Correlated edge overlaps in multiplex networks

    NASA Astrophysics Data System (ADS)

    Baxter, Gareth J.; Bianconi, Ginestra; da Costa, Rui A.; Dorogovtsev, Sergey N.; Mendes, José F. F.

    2016-07-01

    We develop the theory of sparse multiplex networks with partially overlapping links based on their local treelikeness. This theory enables us to find the giant mutually connected component in a two-layer multiplex network with arbitrary correlations between connections of different types. We find that correlations between the overlapping and nonoverlapping links markedly change the phase diagram of the system, leading to multiple hybrid phase transitions. For assortative correlations we observe recurrent hybrid phase transitions.

  16. Pattern formation in multiplex networks

    PubMed Central

    Kouvaris, Nikos E.; Hata, Shigefumi; Guilera, Albert Díaz-

    2015-01-01

    The advances in understanding complex networks have generated increasing interest in dynamical processes occurring on them. Pattern formation in activator-inhibitor systems has been studied in networks, revealing differences from the classical continuous media. Here we study pattern formation in a new framework, namely multiplex networks. These are systems where activator and inhibitor species occupy separate nodes in different layers. Species react across layers but diffuse only within their own layer of distinct network topology. This multiplicity generates heterogeneous patterns with significant differences from those observed in single-layer networks. Remarkably, diffusion-induced instability can occur even if the two species have the same mobility rates; condition which can never destabilize single-layer networks. The instability condition is revealed using perturbation theory and expressed by a combination of degrees in the different layers. Our theory demonstrates that the existence of such topology-driven instabilities is generic in multiplex networks, providing a new mechanism of pattern formation. PMID:26042606

  17. Method and Apparatus of Multiplexing and Acquiring Data from Multiple Optical Fibers Using a Single Data Channel of an Optical Frequency-Domain Reflectometry (OFDR) System

    NASA Technical Reports Server (NTRS)

    Parker, Jr., Allen R (Inventor); Chan, Hon Man (Inventor); Piazza, Anthony (Nino) (Inventor); Richards, William Lance (Inventor)

    2014-01-01

    A method and system for multiplexing a network of parallel fiber Bragg grating (FBG) sensor-fibers to a single acquisition channel of a closed Michelson interferometer system via a fiber splitter by distinguishing each branch of fiber sensors in the spatial domain. On each branch of the splitter, the fibers have a specific pre-determined length, effectively separating each branch of fiber sensors spatially. In the spatial domain the fiber branches are seen as part of one acquisition channel on the interrogation system. However, the FBG-reference arm beat frequency information for each fiber is retained. Since the beat frequency is generated between the reference arm, the effective fiber length of each successive branch includes the entire length of the preceding branch. The multiple branches are seen as one fiber having three segments where the segments can be resolved. This greatly simplifies optical, electronic and computational complexity, and is especially suited for use in multiplexed or branched OFS networks for SHM of large and/or distributed structures which need a lot of measurement points.

  18. Design architectures for optically multiplexed imaging.

    PubMed

    Shepard, R Hamilton; Rachlin, Yaron; Shah, Vinay; Shih, Tina

    2015-11-30

    Optically multiplexed imaging is the process by which multiple images are overlaid on a single image surface. Uniquely encoding the discrete images allows scene reconstruction from multiplexed images via post processing. We describe a class of optical systems that can achieve high density image multiplexing through a novel division of aperture technique. Fundamental design considerations and performance attributes for this sensor architecture are discussed. A number of spatial and temporal encoding methods are presented including point spread function engineering, amplitude modulation, and image shifting. Results from a prototype five-channel sensor are presented using three different encoding methods in sparse-scene star tracking demonstration. A six-channel optically multiplexed prototype sensor is used to reconstruct imagery from information rich dense scenes through dynamic image shifting. PMID:26698767

  19. FPGA-based multi-channel fluorescence lifetime analysis of Fourier multiplexed frequency-sweeping lifetime imaging.

    PubMed

    Zhao, Ming; Li, Yu; Peng, Leilei

    2014-09-22

    We report a fast non-iterative lifetime data analysis method for the Fourier multiplexed frequency-sweeping confocal FLIM (Fm-FLIM) system [Opt. Express 22, 10221 (2014)]. The new method, named R-method, allows fast multi-channel lifetime image analysis in the system's FPGA data processing board. Experimental tests proved that the performance of the R-method is equivalent to that of single-exponential iterative fitting, and its sensitivity is well suited for time-lapse FLIM-FRET imaging of live cells, for example cyclic adenosine monophosphate (cAMP) level imaging with GFP-Epac-mCherry sensors. With the R-method and its FPGA implementation, multi-channel lifetime images can now be generated in real time on the multi-channel frequency-sweeping FLIM system, and live readout of FRET sensors can be performed during time-lapse imaging.

  20. The NA62 Liquid Krypton calorimeter readout architecture

    NASA Astrophysics Data System (ADS)

    Ceccucci, A.; Fantechi, R.; Farthouat, P.; Ryjov, V.; De Simone, N.; Venditti, S.

    2016-01-01

    The NA62 experiment [1] at the CERN SPS (Super Proton Synchrotron) accelerator studies the ultra-rare decays of charged kaons. The high-resolution Liquid Krypton (LKr) electromagnetic calorimeter of the former NA48 experiment [2] is a key component of the experiment photon-veto system. The new LKr readout system comprises 14,000 14-bit ADC acquisition channels, 432× 1 Gbit Ethernet data request and readout links routed by 28× 10 Gbit network switches to the experiment computer farm, and timing, trigger and control (TTC) distribution system. This paper presents the architecture of the LKr readout and TTC systems, the overall performance and the first successfully collected experiment physics data.

  1. Multiplexed Engineering in Biology.

    PubMed

    Rogers, Jameson K; Church, George M

    2016-03-01

    Biotechnology is the manufacturing technology of the future. However, engineering biology is complex, and many possible genetic designs must be evaluated to find cells that produce high levels of a desired drug or chemical. Recent advances have enabled the design and construction of billions of genetic variants per day, but evaluation capacity remains limited to thousands of variants per day. Here we evaluate biological engineering through the lens of the design–build–test cycle framework and highlight the role that multiplexing has had in transforming the design and build steps. We describe a multiplexed solution to the ‘test’ step that is enabled by new research. Achieving a multiplexed test step will permit a fully multiplexed engineering cycle and boost the throughput of biobased product development by up to a millionfold.

  2. Multiplex gas chromatography

    NASA Technical Reports Server (NTRS)

    Valentin, Jose R.

    1990-01-01

    The principles of the multiplex gas chromatography (GC) technique, which is a possible candidate for chemical analysis of planetary atmospheres, are discussed. Particular attention is given to the chemical modulators developed by present investigators for multiplex GC, namely, the thermal-desorption, thermal-decomposition, and catalytic modulators, as well as to mechanical modulators. The basic technique of multiplex GC using chemical modulators and a mechanical modulator is demonstrated. It is shown that, with the chemical modulators, only one gas stream consisting of the carrier in combination with the components is being analyzed, resulting in a simplified instrument that requires relatively few consumables. The mechanical modulator demonstrated a direct application of multiplex GC for the analysis of gases in atmosphere of Titan at very low pressures.

  3. Ultralow Magnetic Fields and Gravity Probe B Gyroscope Readout

    NASA Astrophysics Data System (ADS)

    Mester, J. C.; Lockhart, J. M.; Muhlfelder, B.; Murray, D. O.; Taber, M. A.

    We describe the generation of an ultralow magnetic field of < 10-11Tesla in the flight dewar of the Gravity Probe B Relativity Mission. The field was achieved using expanded-superconducting-shield techniques and is maintained with the aid of a magnetic materials control program. A high performance magnetic shield system is required for the proper function of gyroscope readout. The readout system employs a dc SQUID to measure the London moment generated by the superconducting gyro rotor in order to resolve sub-milliarcsecond changes in the gyro spin direction. In addition to a low residual dc magnetic field, attenuation of external field variation is required to be 1012 at the gyro positions. We discuss the measurement of the dc magnetic field and ac attenuation factor and the performance of the readout system

  4. Multimode fiber optic wavelength division multiplexing

    NASA Technical Reports Server (NTRS)

    Spencer, J. L.

    1982-01-01

    Optical wavelength division multiplexing (WDM) systems, with signals transmitted on different wavelengths through a single optical fiber, can have increased bandwidth and fault isolation properties over single wavelength optical systems. Two WDM system designs that might be used with multimode fibers are considered and a general description of the components which could be used to implement the system are given. The components described are sources, multiplexers, demultiplexers, and detectors. Emphasis is given to the demultiplexer technique which is the major developmental component in the WDM system.

  5. First performance results of the ALICE TPC Readout Control Unit 2

    NASA Astrophysics Data System (ADS)

    Zhao, C.; Alme, J.; Alt, T.; Appelshäuser, H.; Bratrud, L.; Castro, A.; Costa, F.; David, E.; Gunji, T.; Kirsch, S.; Kiss, T.; Langøy, R.; Lien, J.; Lippmann, C.; Oskarsson, A.; Rehman, A. Ur; Røed, K.; Röhrich, D.; Sekiguchi, Y.; Stuart, M.; Ullaland, K.; Velure, A.; Yang, S.; Österman, L.

    2016-01-01

    This paper presents the first performance results of the ALICE TPC Readout Control Unit 2 (RCU2). With the upgraded hardware typology and the new readout scheme in FPGA design, the RCU2 is designed to achieve twice the readout speed of the present Readout Control Unit. Design choices such as using the flash-based Microsemi Smartfusion2 FPGA and applying mitigation techniques in interfaces and FPGA design ensure a high degree of radiation tolerance. This paper presents the system level irradiation test results as well as the first commissioning results of the RCU2. Furthermore, it will be concluded with a discussion of the planned updates in firmware.

  6. Downlink data multiplexer

    NASA Technical Reports Server (NTRS)

    Holland, S. Douglas (Inventor); Steele, Glen F. (Inventor); Romero, Denise M. (Inventor); Koudelka, Robert David (Inventor)

    2008-01-01

    A data multiplexer that accommodates both industry standard CCSDS data packets and bits streams and standard IEEE 1394 data is described. The multiplexer provides a statistical allotment of bandwidth to the channels in turn, preferably four, but expandable in increments of four up to sixteen. A microcontroller determines bandwidth requested by the plurality of channels, as well as the bandwidth available, and meters out the available bandwidth on a statistical basis employing flow control to the input channels.

  7. Acoustic trapping as a generic non-contact incubation site for multiplex bead-based assays.

    PubMed

    Tenje, Maria; Xia, Hongyan; Evander, Mikael; Hammarström, Björn; Tojo, Axel; Belák, Sándor; Laurell, Thomas; LeBlanc, Neil

    2015-01-01

    In this study, we show a significantly reduced assay time and a greatly increased bead recovery for a commercial Luminex-based multiplex diagnostic immunoassay by performing all liquid handling steps of the assay protocol in a non-contact acoustic trapping platform. The Luminex assay is designed for detecting antibodies in poultry serum for infectious bursal disease virus, infectious bronchitis virus, Newcastle disease virus and avian reovirus. Here, we show proof-of-concept of a microfluidic system capable of being fully automated and handling samples in a parallel format with a miniature physical footprint where the affinity beads are retained in a non-contact levitated mode in a glass capillary throughout the assay protocol. The different steps are: incubation with the serum sample, secondary antibodies and fluorescent reporters and finally washing to remove any non-specifically bound species. A Luminex 200 instrument was used for the readout. The flow rates applied to the capillary during the initial trapping event and the wash steps were optimised for maximum bead recovery, resulting in a bead recovery of 75% for the complete assay. This can be compared to a bead recovery of approximately 30% when an automatic wash station was used when the assay was performed in the conventional manual format. The time for the incubation steps for a single assay was reduced by more than 50%, without affecting assay performance, since intermediate wash steps became redundant in the continuously perfused bead trapping capillary. We analyzed seven samples, in triplicates, and we can show that the readout of the assay performed in the acoustic trap compared 100% to the control ELISAs (positive or negative readout) and resulted in comparable S/P values as the conventional manual protocol. As the acoustic trapping does not require the particles to have magnetic properties, a greater degree of freedom in selecting microparticles can be provided. In extension, this can provide an

  8. Integrated quantum dot barcode smartphone optical device for wireless multiplexed diagnosis of infected patients.

    PubMed

    Ming, Kevin; Kim, Jisung; Biondi, Mia J; Syed, Abdullah; Chen, Kun; Lam, Albert; Ostrowski, Mario; Rebbapragada, Anu; Feld, Jordan J; Chan, Warren C W

    2015-03-24

    Inorganic nanoparticles are ideal precursors for engineering barcodes for rapidly detecting diseases. Despite advances in the chemical design of these barcodes, they have not advanced to clinical use because they lack sensitivity and are not cost-effective due to requirement of a large read-out system. Here we combined recent advances in quantum dot barcode technology with smartphones and isothermal amplification to engineer a simple and low-cost chip-based wireless multiplex diagnostic device. We characterized the analytical performance of this device and demonstrated that the device is capable of detecting down to 1000 viral genetic copies per milliliter, and this enabled the diagnosis of patients infected with HIV or hepatitis B. More importantly, the barcoding enabled us to detect multiple infectious pathogens simultaneously, in a single test, in less than 1 h. This multiplexing capability of the device enables the diagnosis of infections that are difficult to differentiate clinically due to common symptoms such as a fever or rash. The integration of quantum dot barcoding technology with a smartphone reader provides a capacity for global surveillance of infectious diseases and the potential to accelerate knowledge exchange transfer of emerging or exigent disease threats with healthcare and military organizations in real time. PMID:25661584

  9. Multiplexed analysis using time-resolved near-IR fluorescence for the detection of genomic material

    NASA Astrophysics Data System (ADS)

    Stryjewski, Wieslaw J.; Soper, Steven A.; Lassiter, Suzzane; Davis, Lloyd M.

    2002-06-01

    While fluorescence continues to be an important tool in genomics, new challenges are being encountered due to increased efforts toward miniaturization reducing detection volumes and the need for screening multiple targets simultaneously. We have initiated work on developing time- resolved near-IR fluorescence as an additional tool for the multiplexed analyses of DNA, either for sequencing or mutation detection. We have fabricated simple and compact time-resolved fluorescence microscopes for reading fluorescence from electrophoresis or DNA microarrays. These microscopes consist of solid-state diode lasers and diode detectors and due to their compact size, the optical components and laser head can be mounted on high-speed micro-translational stages to read fluorescence from either multi-channel capillary electrophoresis instruments or micro fabricated DNA sorting devices. The detector is configured in a time-correlated single photon counting format to allow acquisition of fluorescence lifetimes on-the-fly during data acquisition in the limit of low counting statistics. In multiplexed analyses, lifetime discrimination serves as a method for dye-reporter identification using only a single readout channel. Also, coupled to multi-color systems, lifetime identification can significantly increase the number of probes monitored in a single instrument. In this work, near-IR fluorescence, including dye-labels and hardware, will be discussed as well as the implementation of near-IR fluorescence in DNA sequencing using slab gel electrophoresis and DNA microarrays.

  10. A 16 channel frequency-domain-modulation readout system with custom superconducting LC filters for the SWIPE instrument of the balloon-borne LSPE experiment

    NASA Astrophysics Data System (ADS)

    Signorelli, G.; Baldini, A. M.; Bemporad, C.; Biasotti, M.; Cei, F.; Ceriale, V.; Corsini, D.; Fontanelli, F.; Galli, L.; Gallucci, G.; Gatti, F.; Incagli, M.; Grassi, M.; Nicolò, D.; Spinella, F.; Vaccaro, D.; Venturini, M.

    2016-07-01

    We present the design, implementation and first tests of the superconducting LC filters for the frequency domain readout of spiderweb TES bolometers of the SWIPE experiment on the balloon-borne LSPE mission which aims at measuring the linear polarization of the Cosmic Microwave Background at large angular scales to find the imprint of inflation on the B-mode CMB polarization. LC filters are designed, produced and tested at the INFN sections of Pisa and Genoa where thin film deposition and cryogenic test facilities are present, and where also the TES spiderweb bolometers are being produced.

  11. FPGA-based multi-channel fluorescence lifetime analysis of Fourier multiplexed frequency-sweeping lifetime imaging

    PubMed Central

    Zhao, Ming; Li, Yu; Peng, Leilei

    2014-01-01

    We report a fast non-iterative lifetime data analysis method for the Fourier multiplexed frequency-sweeping confocal FLIM (Fm-FLIM) system [ Opt. Express22, 10221 ( 2014)24921725]. The new method, named R-method, allows fast multi-channel lifetime image analysis in the system’s FPGA data processing board. Experimental tests proved that the performance of the R-method is equivalent to that of single-exponential iterative fitting, and its sensitivity is well suited for time-lapse FLIM-FRET imaging of live cells, for example cyclic adenosine monophosphate (cAMP) level imaging with GFP-Epac-mCherry sensors. With the R-method and its FPGA implementation, multi-channel lifetime images can now be generated in real time on the multi-channel frequency-sweeping FLIM system, and live readout of FRET sensors can be performed during time-lapse imaging. PMID:25321778

  12. A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles

    PubMed Central

    Garai, Ellis; Loewke, Nathan O.; Rogalla, Stephan; Mandella, Michael J.; Felt, Stephen A.; Friedland, Shai; Liu, Jonathan T. C.; Gambhir, Sanjiv S.; Contag, Christopher H.

    2015-01-01

    The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e.g., colon and esophagus) and produces quantitative images of the relative concentrations of SERS NPs that are present. Human and swine studies have demonstrated the speed and simplicity of this technique. This approach also offers unparalleled multiplexing capabilities by simultaneously detecting the unique spectral fingerprints of multiple SERS NPs. Therefore, this new screening strategy has the potential to improve diagnosis and to guide therapy by enabling sensitive quantitative molecular detection of small and otherwise hard-to-detect lesions in the context of white-light endoscopy. PMID:25923788

  13. Design and Evaluation of a Clock Multiplexing Circuit for the SSRL Booster Accelerator Timing System - Oral Presentation

    SciTech Connect

    Araya, Million

    2015-08-25

    SPEAR3 is a 234 m circular storage ring at SLAC’s synchrotron radiation facility (SSRL) in which a 3 GeV electron beam is stored for user access. Typically the electron beam decays with a time constant of approximately 10hr due to electron lose. In order to replenish the lost electrons, a booster synchrotron is used to accelerate fresh electrons up to 3GeV for injection into SPEAR3. In order to maintain a constant electron beam current of 500mA, the injection process occurs at 5 minute intervals. At these times the booster synchrotron accelerates electrons for injection at a 10Hz rate. A 10Hz 'injection ready' clock pulse train is generated when the booster synchrotron is operating. Between injection intervalswhere the booster is not running and hence the 10 Hz ‘injection ready’ signal is not present-a 10Hz clock is derived from the power line supplied by Pacific Gas and Electric (PG&E) to keep track of the injection timing. For this project I constructed a multiplexing circuit to 'switch' between the booster synchrotron 'injection ready' clock signal and PG&E based clock signal. The circuit uses digital IC components and is capable of making glitch-free transitions between the two clocks. This report details construction of a prototype multiplexing circuit including test results and suggests improvement opportunities for the final design.

  14. Design and Evaluation of a Clock Multiplexing Circuit for the SSRL Booster Accelerator Timing System - Final Paper

    SciTech Connect

    Araya, Million

    2015-08-21

    SPEAR3 is a 234 m circular storage ring at SLAC’s synchrotron radiation facility (SSRL) in which a 3 GeV electron beam is stored for user access. Typically the electron beam decays with a time constant of approximately 10hr due to electron lose. In order to replenish the lost electrons, a booster synchrotron is used to accelerate fresh electrons up to 3GeV for injection into SPEAR3. In order to maintain a constant electron beam current of 500mA, the injection process occurs at 5 minute intervals. At these times the booster synchrotron accelerates electrons for injection at a 10Hz rate. A 10Hz 'injection ready' clock pulse train is generated when the booster synchrotron is operating. Between injection intervals-where the booster is not running and hence the 10 Hz ‘injection ready’ signal is not present-a 10Hz clock is derived from the power line supplied by Pacific Gas and Electric (PG&E) to keep track of the injection timing. For this project I constructed a multiplexing circuit to 'switch' between the booster synchrotron 'injection ready' clock signal and PG&E based clock signal. The circuit uses digital IC components and is capable of making glitch-free transitions between the two clocks. This report details construction of a prototype multiplexing circuit including test results and suggests improvement opportunities for the final design.

  15. A Readout Mechanism for Latency Codes

    PubMed Central

    Zohar, Oran; Shamir, Maoz

    2016-01-01

    Response latency has been suggested as a possible source of information in the central nervous system when fast decisions are required. The accuracy of latency codes was studied in the past using a simplified readout algorithm termed the temporal-winner-take-all (tWTA). The tWTA is a competitive readout algorithm in which populations of neurons with a similar decision preference compete, and the algorithm selects according to the preference of the population that reaches the decision threshold first. It has been shown that this algorithm can account for accurate decisions among a small number of alternatives during short biologically relevant time periods. However, one of the major points of criticism of latency codes has been that it is unclear how can such a readout be implemented by the central nervous system. Here we show that the solution to this long standing puzzle may be rather simple. We suggest a mechanism that is based on reciprocal inhibition architecture, similar to that of the conventional winner-take-all, and show that under a wide range of parameters this mechanism is sufficient to implement the tWTA algorithm. This is done by first analyzing a rate toy model, and demonstrating its ability to discriminate short latency differences between its inputs. We then study the sensitivity of this mechanism to fine-tuning of its initial conditions, and show that it is robust to wide range of noise levels in the initial conditions. These results are then generalized to a Hodgkin-Huxley type of neuron model, using numerical simulations. Latency codes have been criticized for requiring a reliable stimulus-onset detection mechanism as a reference for measuring latency. Here we show that this frequent assumption does not hold, and that, an additional onset estimator is not needed to trigger this simple tWTA mechanism. PMID:27812332

  16. FASTBUS Readout Controller card for high speed data acquisition

    SciTech Connect

    Zimmermann, S. Rio Grande do Sul Univ., Porto Alegre, RS . Dept. of Electrical Engineering); Areti, V.H.; Foster, G.W.; Joshi, U.; Treptow, K. )

    1991-10-01

    This article describes a FASTBUS Readout Controller (FRC) for high speed data acquisition in FASTBUS based systems. The controller has two main interfaces: to FASTBUS and to a Readout Port. The FASTBUS interface performs FASTBUS master and slave operations at a maximum transfer rate exceeding 40 MBytes/s. The Readout Port can be adapted for a variety of protocols. Currently, it will be interfaced to a VME bus based processor with a VSB port. The on-board LR33000 embedded processor controls the readout, executing a list of operations download into its memory. It scans the FASTBUS modules and stores the data in a triple port DRAM (TPDRAM), through one of the Serial Access Memory (SAM) ports of the (TPDRAM). Later, it transfers this data to the readout port using the other SAM. The FRC also supports serial communication via RS232 and Ethernet interfaces. This device is intended for use in the data acquisition system at the Collider Detector at Fermilab. 5 refs., 3 figs.

  17. Hyper Suprime-Cam: development of the CCD readout electronics

    NASA Astrophysics Data System (ADS)

    Nakaya, Hidehiko; Uchida, Tomohisa; Miyatake, Hironao; Fujimori, Hiroki; Mineo, Sogo; Aihara, Hiroaki; Furusawa, Hisanori; Kamata, Yukiko; Karoji, Hiroshi; Kawanomoto, Satoshi; Komiyama, Yutaka; Miyazaki, Satoshi; Morokuma, Tomoki; Obuchi, Yoshiyuki; Okura, Yuki; Tanaka, Manobu; Tanaka, Yoko; Uraguchi, Fumihiro; Utsumi, Yosuke

    2010-07-01

    Hyper Suprime-Cam (HSC) employs 116 of 2k×4k CCDs with 464 signal outputs in total. The image size exceeds 2 GBytes, and the data can be readout every 10 seconds which results in the data rate of 210 Mbytes / sec. The data is digitized to 16-bit. The readout noise of the electronics at the readout time of 20 seconds is ~0.9 ADU, and the one with CCD is ~1.5 ADU which corresponds to ~4.5 e. The linearity error fits within +/- 0.5 % up to 150,000 e. The CCD readout electronics for HSC was newly developed based on the electronics for Suprime-Cam. The frontend electronics (FEE) is placed in the vacuum dewar, and the backend electronics (BEE) is mounted on the outside of the dewar on the prime focus unit. The FEE boards were designed to minimize the outgas and to maximize the heat transfer efficiency to keep the vacuum of the dewar. The BEE boards were designed to be simple and small as long as to achieve the readout time within 10 seconds. The production of the system has been finished, and the full set of the boards are being tested with several CCDs installed in the HSC dewar. We will show the system design, performance, and the current status of the development.

  18. Development of arrays of Silicon Drift Detectors and readout ASIC for the SIDDHARTA experiment

    NASA Astrophysics Data System (ADS)

    Quaglia, R.; Schembari, F.; Bellotti, G.; Butt, A. D.; Fiorini, C.; Bombelli, L.; Giacomini, G.; Ficorella, F.; Piemonte, C.; Zorzi, N.

    2016-07-01

    This work deals with the development of new Silicon Drift Detectors (SDDs) and readout electronics for the upgrade of the SIDDHARTA experiment. The detector is based on a SDDs array organized in a 4×2 format with each SDD square shaped with 64 mm2 (8×8) active area. The total active area of the array is therefore 32×16 mm2 while the total area of the detector (including 1 mm border dead area) is 34 × 18mm2. The SIDDHARTA apparatus requires 48 of these modules that are designed and manufactured by Fondazione Bruno Kessler (FBK). The readout electronics is composed by CMOS preamplifiers (CUBEs) and by the new SFERA (SDDs Front-End Readout ASIC) circuit. SFERA is a 16-channels readout ASIC designed in a 0.35 μm CMOS technology, which features in each single readout channel a high order shaping amplifier (9th order Semi-Gaussian complex-conjugate poles) and a high efficiency pile-up rejection logic. The outputs of the channels are connected to an analog multiplexer for the external analog to digital conversion. An on-chip 12-bit SAR ADC is also included. Preliminary measurements of the detectors in the single SDD format are reported. Also measurements of low X-ray energies are reported in order to prove the possible extension to the soft X-ray range.

  19. Compact spatial multiplexers for mode division multiplexing.

    PubMed

    Chen, Haoshuo; van Uden, Roy; Okonkwo, Chigo; Koonen, Ton

    2014-12-29

    Spatial multiplexer (SMUX) for mode division multiplexing (MDM) has evolved from mode-selective excitation, multiple-spot and photonic-lantern based solutions in order to minimize both mode-dependent loss (MDL) and coupler insertion loss (CIL). This paper discusses the implementation of all the three solutions by compact components in a small footprint. Moreover, the compact SMUX can be manufactured in mass production and packaged to assure high reliability. First, push-pull scheme and center launch based SMUXes are demonstrated on two mostly-popular photonic integration platforms: Silicon-on-insulator (SOI) and Indium Phosphide (InP) for selectively exciting LP01 and LP11 modes. 2-dimensional (2D) top-coupling by using vertical emitters is explored to provide a coupling interface between a few-mode fiber (FMF) and the photonic integrated SMUX. SOI-based grating couplers and InP-based 45° vertical mirrors are proposed and researched as vertical emitters in each platform. Second, a 3-spot SMUX is realized on an InP-based circuit through employing 45° vertical mirrors. Third, as a newly-emerging photonic integration platform, laser-inscribed 3D waveguide (3DW) technology is applied for a fully-packaged dual-channel 6-mode SMUX including two 6-core photonic lantern structures as mode multiplexer and demultiplexer, respectively.

  20. Numerical simulations on the focus-shift multiplexing technique for self-referential holographic data storage

    NASA Astrophysics Data System (ADS)

    Takabayashi, Masanori; Eto, Taisuke; Okamoto, Takashi

    2016-08-01

    For increasing the data density of holographic data storage (HDS), combining more than two multiplexing techniques is effective. This is also true in self-referential holographic data storage (SR-HDS) that enables holographic recording purely with a single beam. In this paper, a focus-shift multiplexing technique is applied to xy -shift multiplexed SR-HDS, the feasibility of which has been shown in our previous work. The focus-shift multiplexing technique enables the multiplexing of datapages by slightly changing the focal length of the objective lens. However, the required focus-shift distance for multiplexing and the implementation method of the focus-shift have not been clarified. First, the focus-shift selectivity is investigated by the numerical simulations. In the case where the focus-shift multiplexing technique is applied to xy -shift multiplexed SR-HDS, the inter-page crosstalk properties are clarified to decide the recording layout that can achieve a low-crosstalk readout. Second, the technique of displaying an additional phase pattern onto the spatial light modulator (SLM) is introduced, which is a focus-shift method without any special optical components, such as varifocal lenses. Finally, we investigate the relationship between the accuracy of the focus-shift and the parameters of SLM.

  1. Multispecies absorption spectroscopy of detonation events at 100  kHz using a fiber-coupled, time-division-multiplexed quantum-cascade-laser system.

    PubMed

    Rein, Keith D; Roy, Sukesh; Sanders, Scott T; Caswell, Andrew W; Schauer, Frederick R; Gord, James R

    2016-08-10

    A mid-infrared fiber-coupled laser system constructed around three time-division-multiplexed quantum-cascade lasers capable of measuring the absorption spectra of CO, CO2, and N2O at 100 kHz over a wide range of operating pressures and temperatures is demonstrated. This system is first demonstrated in a laboratory burner and then used to measure temperature, pressure, and concentrations of CO, CO2, and N2O as a function of time in a detonated mixture of N2O and C3H8. Both fuel-rich and fuel-lean detonation cases are outlined. High-temperature fluctuations during the blowdown are observed. Concentrations of CO are shown to decrease with time for fuel-lean conditions and increase for fuel-rich conditions.

  2. Multispecies absorption spectroscopy of detonation events at 100  kHz using a fiber-coupled, time-division-multiplexed quantum-cascade-laser system.

    PubMed

    Rein, Keith D; Roy, Sukesh; Sanders, Scott T; Caswell, Andrew W; Schauer, Frederick R; Gord, James R

    2016-08-10

    A mid-infrared fiber-coupled laser system constructed around three time-division-multiplexed quantum-cascade lasers capable of measuring the absorption spectra of CO, CO2, and N2O at 100 kHz over a wide range of operating pressures and temperatures is demonstrated. This system is first demonstrated in a laboratory burner and then used to measure temperature, pressure, and concentrations of CO, CO2, and N2O as a function of time in a detonated mixture of N2O and C3H8. Both fuel-rich and fuel-lean detonation cases are outlined. High-temperature fluctuations during the blowdown are observed. Concentrations of CO are shown to decrease with time for fuel-lean conditions and increase for fuel-rich conditions. PMID:27534467

  3. Multiplexed analysis of genes using nucleic acid-stabilized silver-nanocluster quantum dots.

    PubMed

    Enkin, Natalie; Wang, Fuan; Sharon, Etery; Albada, H Bauke; Willner, Itamar

    2014-11-25

    Luminescent nucleic acid-stabilized Ag nanoclusters (Ag NCs) are applied for the optical detection of DNA and for the multiplexed analysis of genes. Two different sensing modules including Ag NCs as luminescence labels are described. One sensing module involves the assembly of a three-component sensing module composed of a nucleic acid-stabilized Ag NC and a quencher-modified nucleic acid hybridized with a nucleic acid scaffold that is complementary to the target DNA. The luminescence of the Ag NCs is quenched in the sensing module nanostructure. The strand displacement of the scaffold by the target DNA separates the nucleic acid-functionalized Ag NCs, leading to the turned-on luminescence of the NCs and to the optical readout of the sensing process. By implementing two different-sized Ag NC-modified sensing modules, the parallel multiplexed analysis of two genes (the Werner Syndrome gene and the HIV, human immunodeficiency, gene), using 615 and 560 nm luminescent Ag NCs, is demonstrated. The second sensing module includes the nucleic acid functionalized Ag NCs and the quencher-modified nucleic acid hybridized with a hairpin DNA scaffold. The luminescence of the Ag NCs is quenched in the sensing module. Opening of the hairpin by the target DNA triggers the luminescence of the Ag NCs, due to the spatial separation of the Ag NCs/quencher units. The system is applied for the optical detection of the BRAC1 gene. In addition, by implementing two-sized Ag NCs, the multiplexed analysis of two genes by the hairpin sensing module approach is demonstrated.

  4. Cascading processes on multiplex networks: Impact of weak layers

    NASA Astrophysics Data System (ADS)

    Lee, Kyu-Min; Goh, Kwang-Il

    Many real-world complex systems such as biological and socio-technological systems consist of manifold layers in multiplex networks. The multiple network layers give rise to the nonlinear effect for the emergent dynamics of systems. Especially, the weak layers plays the significant role in nonlinearity of multiplex networks, which can be neglected in single-layer network framework overlaying all layers. Here we present a simple model of cascades on multiplex networks of heterogeneous layers. The model is simulated on the multiplex network of international trades. We found that the multiplex model produces more catastrophic cascading failures which were the result of collective behaviors from coupling layers rather than the simple summation effect. Therefore risks can be systematically underestimated in simply overlaid network system because the impact of weak layers is overlooked. Our simple theoretical model would have some implications to investigate and design optimal real-world complex systems.

  5. A generic readout environment for prototype pixel detectors

    NASA Astrophysics Data System (ADS)

    Turqueti, Marcos; Rivera, Ryan; Prosser, Alan; Kwan, Simon

    2010-11-01

    Pixel detectors for experimental particle physics research have been implemented with a variety of readout formats and potentially generate massive amounts of data. Examples include the PSI46 device for the Compact Muon Solenoid (CMS) experiment which implements an analog readout, the Fermilab FPIX2.1 device with a digital readout, and the Fermilab Vertically Integrated Pixel device. The Electronic Systems Engineering Department of the Computing Division at the Fermi National Accelerator Laboratory has developed a data acquisition system flexible and powerful enough to meet the various needs of these devices to support laboratory test bench as well as test beam applications. The system is called CAPTAN (Compact And Programmable daTa Acquisition Node) and is characterized by its flexibility, versatility and scalability by virtue of several key architectural features. These include a vertical bus that permits the user to stack multiple boards, a gigabit Ethernet link that permits high speed communications to the system and a core group of boards that provide specific processing and readout capabilities for the system. System software based on distributed computing techniques supports an expandable network of CAPTANs. In this paper, we describe the system architecture and give an overview of its capabilities.

  6. Multiplexed Colorimetric Solid-Phase Extraction

    NASA Technical Reports Server (NTRS)

    Gazda, Daniel B.; Fritz, James S.; Porter, Marc D.

    2009-01-01

    Multiplexed colorimetric solid-phase extraction (MC-SPE) is an extension of colorimetric solid-phase extraction (C-SPE) an analytical platform that combines colorimetric reagents, solid phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water. In CSPE, analytes are extracted and complexed on the surface of an extraction membrane impregnated with a colorimetric reagent. The analytes are then quantified directly on the membrane surface using a handheld diffuse reflectance spectrophotometer. Importantly, the use of solid-phase extraction membranes as the matrix for impregnation of the colorimetric reagents creates a concentration factor that enables the detection of low concentrations of analytes in small sample volumes. In extending C-SPE to a multiplexed format, a filter holder that incorporates discrete analysis channels and a jig that facilitates the concurrent operation of multiple sample syringes have been designed, enabling the simultaneous determination of multiple analytes. Separate, single analyte membranes, placed in a readout cartridge create unique, analyte-specific addresses at the exit of each channel. Following sample exposure, the diffuse reflectance spectrum of each address is collected serially and the Kubelka-Munk function is used to quantify each water quality parameter via calibration curves. In a demonstration, MC-SPE was used to measure the pH of a sample and quantitate Ag(I) and Ni(II).

  7. Mesoscopic quantum multiplex for channeling bunches

    NASA Astrophysics Data System (ADS)

    Shen, Jing

    1998-09-01

    (1) Bogacz-Cline channeling is an interesting idea that can transform a bunch of low particle intensity to a collider of high luminosity but it was maintained as impossible to carry out because of three technical problems. (2) The first of which is discussed in this paper, and it is how to get billions particles from each bunch to enter into and channel through a single crystal channel. (3) Two basic difficulties of entrance are discussed in this paper. The first is due to the Heisenberg's uncertainty, and the second is the dimension reduction of a beam bunch in crystal from 3D to 1D. (4) To overcome these difficulties, a hybrid device named Mesoscopic Quantum Multiplex (MQM) is designed to achieve entrance and channeling. It is a quantum generalization of classical multiplex in detector readout electronics for the classical-quantum interface. It is made by nano-crystalline technology. (5) The MQM can channel the Richter-Kimura-Takada flat e± beams of NLC-JLC, and low emittance p or heavy ion beams as well as the Bogacz-Cline μ± beams, and the Nagamine-Chu cool μ± beams.

  8. Pixel multiplexing technique for real-time three-dimensional-imaging laser detection and ranging system using four linear-mode avalanche photodiodes.

    PubMed

    Xu, Fan; Wang, Yuanqing; Li, Fenfang

    2016-03-01

    The avalanche-photodiode-array (APD-array) laser detection and ranging (LADAR) system has been continually developed owing to its superiority of nonscanning, large field of view, high sensitivity, and high precision. However, how to achieve higher-efficient detection and better integration of the LADAR system for real-time three-dimensional (3D) imaging continues to be a problem. In this study, a novel LADAR system using four linear mode APDs (LmAPDs) is developed for high-efficient detection by adopting a modulation and multiplexing technique. Furthermore, an automatic control system for the array LADAR system is proposed and designed by applying the virtual instrumentation technique. The control system aims to achieve four functions: synchronization of laser emission and rotating platform, multi-channel synchronous data acquisition, real-time Ethernet upper monitoring, and real-time signal processing and 3D visualization. The structure and principle of the complete system are described in the paper. The experimental results demonstrate that the LADAR system is capable of achieving real-time 3D imaging on an omnidirectional rotating platform under the control of the virtual instrumentation system. The automatic imaging LADAR system utilized only 4 LmAPDs to achieve 256-pixel-per-frame detection with by employing 64-bit demodulator. Moreover, the lateral resolution is ∼15 cm and range accuracy is ∼4 cm root-mean-square error at a distance of ∼40 m.

  9. Pixel multiplexing technique for real-time three-dimensional-imaging laser detection and ranging system using four linear-mode avalanche photodiodes

    NASA Astrophysics Data System (ADS)

    Xu, Fan; Wang, Yuanqing; Li, Fenfang

    2016-03-01

    The avalanche-photodiode-array (APD-array) laser detection and ranging (LADAR) system has been continually developed owing to its superiority of nonscanning, large field of view, high sensitivity, and high precision. However, how to achieve higher-efficient detection and better integration of the LADAR system for real-time three-dimensional (3D) imaging continues to be a problem. In this study, a novel LADAR system using four linear mode APDs (LmAPDs) is developed for high-efficient detection by adopting a modulation and multiplexing technique. Furthermore, an automatic control system for the array LADAR system is proposed and designed by applying the virtual instrumentation technique. The control system aims to achieve four functions: synchronization of laser emission and rotating platform, multi-channel synchronous data acquisition, real-time Ethernet upper monitoring, and real-time signal processing and 3D visualization. The structure and principle of the complete system are described in the paper. The experimental results demonstrate that the LADAR system is capable of achieving real-time 3D imaging on an omnidirectional rotating platform under the control of the virtual instrumentation system. The automatic imaging LADAR system utilized only 4 LmAPDs to achieve 256-pixel-per-frame detection with by employing 64-bit demodulator. Moreover, the lateral resolution is ˜15 cm and range accuracy is ˜4 cm root-mean-square error at a distance of ˜40 m.

  10. Pixel multiplexing technique for real-time three-dimensional-imaging laser detection and ranging system using four linear-mode avalanche photodiodes.

    PubMed

    Xu, Fan; Wang, Yuanqing; Li, Fenfang

    2016-03-01

    The avalanche-photodiode-array (APD-array) laser detection and ranging (LADAR) system has been continually developed owing to its superiority of nonscanning, large field of view, high sensitivity, and high precision. However, how to achieve higher-efficient detection and better integration of the LADAR system for real-time three-dimensional (3D) imaging continues to be a problem. In this study, a novel LADAR system using four linear mode APDs (LmAPDs) is developed for high-efficient detection by adopting a modulation and multiplexing technique. Furthermore, an automatic control system for the array LADAR system is proposed and designed by applying the virtual instrumentation technique. The control system aims to achieve four functions: synchronization of laser emission and rotating platform, multi-channel synchronous data acquisition, real-time Ethernet upper monitoring, and real-time signal processing and 3D visualization. The structure and principle of the complete system are described in the paper. The experimental results demonstrate that the LADAR system is capable of achieving real-time 3D imaging on an omnidirectional rotating platform under the control of the virtual instrumentation system. The automatic imaging LADAR system utilized only 4 LmAPDs to achieve 256-pixel-per-frame detection with by employing 64-bit demodulator. Moreover, the lateral resolution is ∼15 cm and range accuracy is ∼4 cm root-mean-square error at a distance of ∼40 m. PMID:27036763

  11. Multiplexed Biosensors for Mycotoxins.

    PubMed

    Maragos, Chris M

    2016-07-01

    Significant progress has been made in the development of biosensors that can be used to detect low-MW toxins produced by fungi (mycotoxins). The number of formats that have been investigated is impressive and is an indication of the importance attached to finding easy-to-use, accurate, and rapid methods for detecting these toxins in commodities and foods. This review explores the details of multiplexed biosensors based on many formats, including multiplexed immunoassays, suspension arrays, membrane-based devices (flow-through and immunochromatographic), and planar microarrays. Each assay format has its own strengths and areas that need improvement. Certain formats, such as multiplexed immunochromatographic devices, are well developed and relatively easy to use, and in some cases, commercial products are being sold. Others, such as the suspension arrays and microarrays, are laboratory-based assays that, although more complicated, are also more amenable to a larger scale of multiplexing. The diversity of such efforts and the multitude of formats under investigation suggest that multiple solutions will be found to satisfy the need for multiplexed toxin detection. PMID:27455928

  12. Looking at Earth from space: Direct readout from environmental satellites

    NASA Technical Reports Server (NTRS)

    1994-01-01

    Direct readout is the capability to acquire information directly from meteorological satellites. Data can be acquired from NASA-developed, National Oceanic and Atmospheric Administration (NOAA)-operated satellites, as well as from other nations' meteorological satellites. By setting up a personal computer-based ground (Earth) station to receive satellite signals, direct readout may be obtained. The electronic satellite signals are displayed as images on the computer screen. The images can display gradients of the Earth's topography and temperature, cloud formations, the flow and direction of winds and water currents, the formation of hurricanes, the occurrence of an eclipse, and a view of Earth's geography. Both visible and infrared images can be obtained. This booklet introduces the satellite systems, ground station configuration, and computer requirements involved in direct readout. Also included are lists of associated resources and vendors.

  13. Point-of-care multiplexed assays of nucleic acids using microcapillary-based loop-mediated isothermal amplification.

    PubMed

    Zhang, Yi; Zhang, Lu; Sun, Jiashu; Liu, Yulei; Ma, Xingjie; Cui, Shangjin; Ma, Liying; Xi, Jianzhong Jeff; Jiang, Xingyu

    2014-07-15

    This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings. PMID:24937125

  14. Fully digital readout of segmented solid state detectors

    NASA Astrophysics Data System (ADS)

    Blumenhagen, K. H.; Badura, E.; Bräuning, H.; Hoffmann, J.; Koch, K.; Kurz, N.; Märtin, R.; Minami, S.; Ott, W.; Spillmann, U.; Stöhlker, Th; Weber, G.; Weber, M.

    2013-09-01

    In this work, the digital readout of semiconductor detectors in combination with digital filters was investigated. Both non-segmented high-purity germanium and segmented planar lithium-drifted silicon detectors were used. In each case, photons from a stationary americium (241Am) gamma source were detected. The resulting preamplifier output pulses were digitized at a fixed sampling frequency and stored entirely. Digital filters were applied to the stored waveforms to extract time and energy information. The performance of different digital filters was compared. The optimum energy resolution obtained was comparable with the value resulting from an analogue readout system based on standard nuclear instrumentation module and versatile module Europe bus electronics.

  15. Probing the Speed Limits of Transmon Dispersive Readout

    NASA Astrophysics Data System (ADS)

    Walter, Theo; Kurpiers, Philipp; Mondal, Mintu; Pechal, Marek; Wallraff, Andreas

    In circuit QED, faster and more accurate measurement of a qubit's state is necessary to achieve better feedback control, to accomplish more complex quantum algorithms and simulations, and to cross the threshold for fault tolerant quantum computing. In this talk, we discuss our experimental progress to minimize the time needed to readout the state of a dispersively coupled transmon qubit with high fidelity. We outline a signal-to-noise ratio model, illuminate the constraints and find optimal parameters for maximizing measurement speed, while maintaining high readout fidelity. Utilizing a Purcell Filter increases the generality of our results as it becomes possible to reach these speeds with a broader set of system parameters.

  16. A Pixel Readout Chip in 40 nm CMOS Process for High Count Rate Imaging Systems with Minimization of Charge Sharing Effects

    SciTech Connect

    Maj, Piotr; Grybos, P.; Szczgiel, R.; Kmon, P.; Drozd, A.; Deptuch, G.

    2013-11-07

    We present a prototype chip in 40 nm CMOS technology for readout of hybrid pixel detector. The prototype chip has a matrix of 18x24 pixels with a pixel pitch of 100 m. It can operate both in single photon counting (SPC) mode and in C8P1 mode. In SPC the measured ENC is 84 e rms (for the peaking time of 48 ns), while the effective offset spread is below 2 mV rms. In the C8P1 mode the chip reconstructs full charge deposited in the detector, even in the case of charge sharing, and it identifies a pixel with the largest charge deposition. The chip architecture and preliminary measurements are reported.

  17. Sources of noise in magneto-optical readout

    NASA Technical Reports Server (NTRS)

    Mansuripur, M.

    1991-01-01

    The various sources of noise which are often encountered in magneto-optical readout systems are analyzed. Although the focus is on magneto-optics, most sources of noise are common among the various optical recording systems and one can easily adapt the results to other media and systems. A description of the magneto-optical readout system under consideration is given, and the standard methods and the relevant terminology of signal and noise measurement are described. The characteristics of thermal noise, which originates in the electronic circuitry of the readout system, are described. The most fundamental of all sources of noise, the shot noise, is considered, and a detailed account of its statistical properties is given. Shot noise, which is due to random fluctuations in photon arrival times, is an ever-present noise in optical detection. Since the performance of magneto-optical recording devices in use today is approaching the limit imposed by the shot noise, it is important that the reader have a good grasp of this particular source of noise. A model for the laser noise is described, and measurement results which yield numerical values for the strength of the laser power fluctuations are presented. Spatial variations of the disk reflectivity and random depolarization phenomena also contribute to the overall level of noise in readout; these and related issues are treated. Numerical simulation results describing some of the more frequently encountered sources of noise which accompany the recorded waveform itself, namely, jitter noise and signal-amplitude fluctuation noise are presented.

  18. Analysis of tolerances in polytopic-multiplexing holographic data storage.

    PubMed

    Ide, Tatsuro

    2016-04-10

    A fast Fourier transformation (FFT)-based simulator was developed to analyze a recovered image of a hologram formed by polytopic multiplexing holographic data storage and to determine the positional tolerances of optical devices. By focusing the positional shift of 4f configuration devices (phase mask, SLM, polytopic filter, and camera), the number of FFT iterations was reduced, and the calculation speed was improved. Using the simulator, the positional tolerances of these devices were determined. Also, to validate the simulator, simulated readout images under defocuses of the phase mask and camera were compared with experimentally obtained images and found to agree well. PMID:27139848

  19. Performance analysis of precoding-based asymmetrically clipped optical orthogonal frequency division multiplexing wireless system in additive white Gaussian noise and indoor multipath channel

    NASA Astrophysics Data System (ADS)

    Ranjha, Bilal; Zhou, Zhou; Kavehrad, Mohsen

    2014-08-01

    We have compared the bit error rate (BER) performance of precoding-based asymmetrically clipped optical orthogonal frequency division multiplexing (ACO-OFDM) and pulse amplitude modulated discrete multitone (PAM-DMT) optical wireless (OW) systems in additive white Gaussian noise (AWGN) and indoor multipath frequency selective channel. Simulation and analytical results show that precoding schemes such as discrete Fourier transform, discrete cosine transform, and Zadoff-Chu sequences do not affect the performance of the OW systems in the AWGN channel while they do reduce the peak-to-average power ratio (PAPR) of the OFDM output signal. However, in a multipath indoor channel, using zero forcing frequency domain equalization precoding-based systems give better BER performance than their conventional counterparts. With additional clipping to further reduce the PAPR, precoding-based systems also show better BER performance compared to nonprecoded systems when clipped relative to the peak of nonprecoded systems. Therefore, precoding-based ACO-OFDM and PAM-DMT systems offer better BER performance, zero signaling overhead, and low PAPR compared to conventional systems.

  20. Simultaneous parallel inclined readout image technique.

    PubMed

    Paley, Martyn N J; Lee, Kuan J; Wild, James M; Griffiths, Paul D; Whitby, Elspeth H

    2006-06-01

    Sensitivity-encoded phase undersampling has been combined with simultaneous slice excitation to produce a parallel MRI method with a high volumetric acquisition acceleration factor without the need for auxiliary stepped field coils. Dual-slice excitation was produced by modulating both spin and gradient echo sequences at +/-6 kHz. Frequency aliasing of simultaneously excited slices was prevented by using an additional gradient applied along the slice axis during data acquisition. Data were acquired using a four-channel receiver array and x4 sensitivity encoding on a 1.5 T MR system. The simultaneous parallel inclined readout image technique has been successfully demonstrated in both phantoms and volunteers. A multiplicative image acquisition acceleration factor of up to x8 was achieved. Image SNR and resolution was dependent on the ratio of the readout gradient to the additional slice gradient. A ratio of approximately 2:1 produced acceptable image quality. Use of RF pulses with additional excitation bands should enable the technique to be extended to volumetric acquisition acceleration factors in the range of x16-24 without the SNR limitations of pure partially parallel phase reduction methods.