Putting together a plasma membrane NADH oxidase: a tale of three laboratories.

PubMed

Löw, Hans; Crane, Frederick L; Morré, D James

2012-11-01

The observation that high cellular concentrations of NADH were associated with low adenylate cyclase activity led to a search for the mechanism of the effect. Since cyclase is in the plasma membrane, we considered the membrane might have a site for NADH action, and that NADH might be oxidized at that site. A test for NADH oxidase showed very low activity, which could be increased by adding growth factors. The plasma membrane oxidase was not inhibited by inhibitors of mitochondrial NADH oxidase such as cyanide, rotenone or antimycin. Stimulation of the plasma membrane oxidase by iso-proterenol or triiodothyronine was different from lack of stimulation in endoplasmic reticulum. After 25 years of research, three components of a trans membrane NADH oxidase have been discovered. Flavoprotein NADH coenzyme Q reductases (NADH cytochrome b reductase) on the inside, coenzyme Q in the middle, and a coenzyme Q oxidase on the outside as a terminal oxidase. The external oxidase segment is a copper protein with unique properties in timekeeping, protein disulfide isomerase and endogenous NADH oxidase activity, which affords a mechanism for control of cell growth by the overall NADH oxidase and the remarkable inhibition of oxidase activity and growth of cancer cells by a wide range of anti-tumor drugs. A second trans plasma membrane electron transport system has been found in voltage dependent anion channel (VDAC), which has NADH ferricyanide reductase activity. This activity must be considered in relation to ferricyanide stimulation of growth and increased VDAC antibodies in patients with autism. Copyright © 2012 Elsevier Ltd. All rights reserved.

Genetically encoded probes for NAD+/NADH monitoring.

PubMed

Bilan, Dmitry S; Belousov, Vsevolod V

2016-11-01

NAD + and NADH participate in many metabolic reactions. The NAD + /NADH ratio is an important parameter reflecting the general metabolic and redox state of different types of cells. For a long time, in situ and in vivo NAD + /NADH monitoring has been hampered by the lack of suitable tools. The recent development of genetically encoded indicators based on fluorescent proteins linked to specific nucleotide-binding domains has already helped to address this monitoring problem. In this review, we will focus on four available indicators: Peredox, Frex family probes, RexYFP and SoNar. Each indicator has advantages and limitations. We will also discuss the most important points that should be considered when selecting a suitable indicator for certain experimental conditions. Copyright © 2016 Elsevier Inc. All rights reserved.

Space Shuttle Project

NASA Image and Video Library

1995-03-18

The Space Shuttle Endeavour (STS-67) lands at Edwards Air Force Base in southern California after successfully completing NASA's longest plarned shuttle mission. The seven-member crew conducted round-the-clock observations with the ASTRO-2 observatory, a trio of telescopes designed to study the universe of ultraviolet astronomy. Because of Earth's protective ozone layer ultraviolet light from celestial objects does not reach gound-based telescopes, and such studies can only be conducted from space.

Space Shuttle Projects

NASA Image and Video Library

1994-09-13

Designed by the mission crew members, the STS-66 emblem depicts the Space Shuttle Atlantis launching into Earth orbit to study global environmental change. The payload for the Atmospheric Laboratory for Applications and Science (ATLAS-3) and complementary experiments were part of a continuing study of the atmosphere and the Sun's influence on it. The Space Shuttle is trailed by gold plumes representing the astronaut symbol and is superimposed over Earth, much of which is visible from the flight's high inclination orbit. Sensitive instruments aboard the ATLAS pallet in the Shuttle payload bay and on the free-flying Cryogenic Infrared Spectrometers and Telescopes for the Atmospheric-Shuttle Pallet Satellite (CHRISTA-SPAS) that gazed down on Earth and toward the Sun, are illustrated by the stylized sunrise and visible spectrum.

Study the oxidative injury of yeast cells by NADH autofluorescence

NASA Astrophysics Data System (ADS)

Liang, Ju; Wu, Wen-Lan; Liu, Zhi-Hong; Mei, Yun-Jun; Cai, Ru-Xiu; Shen, Ping

2007-06-01

Autofluorescence has an advantage over the extrinsic fluorescence of an unperturbed environment during investigation, especially in complex system such as biological cells and tissues. NADH is an important fluorescent substance in living cells. The time courses of intracellular NADH autofluorescence in the process of yeast cells exposed to H 2O 2 and ONOO - have been recorded in detail in this work. In the presence of different amounts of H 2O 2 and ONOO -, necrosis, apoptosis and reversible injury are initiated in yeast cells, which are confirmed by acridine orange/ethidum bromide and Annexin V/propidium iodide staining. It is found that intracellular NADH content increases momently in the beginning of the apoptotic process and then decreases continually till the cell dies. The most remarkable difference between the apoptotic and the necrotic process is that the NADH content in the latter case changes much more sharply. Further in the case of reversible injury, the time course of intracellular NADH content is completely different from the above two pathways of cell death. It just decreases to some degree firstly and then resumes to the original level. Based on the role of NADH in mitochondrial respiratory chain, the time course of intracellular NADH content is believed to have reflected the response of mitochondrial redox state to oxidative stress. Thus, it is found that the mitochondrial redox state changes differently in different pathways of oxidative injury in yeast cells.

Purification and Kinetics of Higher Plant NADH:Nitrate Reductase.

PubMed

Campbell, W H; Smarrelli, J

1978-04-01

Squash cotyledon (Cucurbita pepo L.) NADH:nitrate reductase (NR) was purified 150-fold with 50% recovery by a single step procedure based on the affinity of the NR for blue-Sepharose. Blue-Sepharose, which is prepared by direct coupling of Cibacron blue to Sepharose, appears to bind squash NR at the NADH site. The NR can be purified in 2 to 3 hours to a specific activity of 2 mumol of NADH oxidized/minute * milligram of protein. Corn (Zea mays L.) leaf NR was also purified to a specific activity of 6.9 mumol of NADH oxidized/minute * milligram of protein using a blue-Sepharose affinity step. The blue-Sepharose method offers the advantages of a rapid purification of plant NR to a high specific activity with reasonable recovery of total activity.The kinetic mechanism of higher plant NR was investigated using these highly purified squash and corn NR preparations. Based on initial velocity and product inhibition studies utilizing both enzymes, a two-site ping-pong mechanism is proposed for NR. This kinetic mechanism incorporates the concept of the reduced NR transferring electrons from the NADH site to a physically separated nitrate site.

A high effective NADH-ferricyanide dehydrogenase coupled with laccase for NAD(+) regeneration.

PubMed

Wang, Jizhong; Yang, Chengli; Chen, Xing; Bao, Bingxin; Zhang, Xuan; Li, Dali; Du, Xingfan; Shi, Ruofu; Yang, Junfang; Zhu, Ronghui

2016-08-01

To find an efficient and cheap system for NAD(+) regeneration A NADH-ferricyanide dehydrogenase was obtained from an isolate of Escherichia coli. Optimal activity of the NADH dehydrogenase was at 45 °C and pH 7.5, with a K m value for NADH of 10 μM. By combining the NADH dehydrogenase, potassium ferricyanide and laccase, a bi-enzyme system for NAD(+) regeneration was established. The system is attractive in that the O2 consumed by laccase is from air and the sole byproduct of the reaction is water. During the reaction process, 10 mM NAD(+) was transformed from NADH in less than 2 h under the condition of 0.5 U NADH dehydrogenase, 0.5 U laccase, 0.1 mM potassium ferricyanide at pH 5.6, 30 °C CONCLUSION: The bi-enzyme system employed the NADH-ferricyanide dehydrogenase and laccase as catalysts, and potassium ferricyanide as redox mediator, is a promising alternative for NAD(+) regeneration.

Space Shuttle Projects

NASA Image and Video Library

1984-10-01

The Space Shuttle Discovery en route to Earth orbit for NASA's 51-A mission is reminiscent of a soaring Eagle. The red and white trailing stripes and the blue background, along with the presence of the Eagle, generate memories of America's 208 year-old history and traditions. The two satellites orbiting the Earth backgrounded amidst a celestial scene are a universal representation of the versatility of the Space Shuttle. White lettering against the blue border lists the surnames of the five-member crew.

Interaction between NADH and electron-transferring flavoprotein from Megasphaera elsdenii.

PubMed

Sato, Kyosuke; Nishina, Yasuzo; Shiga, Kiyoshi

2013-06-01

Electron-transferring flavoprotein (ETF) from the anaerobic bacterium Megasphaera elsdenii is a heterodimer containing two FAD cofactors. Isolated ETF contains only one FAD molecule, FAD-1, because the other, FAD-2, is lost during purification. FAD-2 is recovered by adding FAD to the isolated ETF. The two FAD molecules in holoETF were characterized using NADH. Spectrophotometric titration of isolated ETF with NADH showed a two-electron reduction of FAD-1 according to a monophasic profile indicating that FAD-1 receives electrons from NADH without involvement of FAD-2. When holoETF was titrated with NADH, FAD-2 was reduced to an anionic semiquinone and then was fully reduced before the reduction of FAD-1. The midpoint potential values at pH 7 were +81, -136 and -279 mV for the reduction of oxidized FAD-2 to semiquinone, semiquinone to the fully reduced FAD-2 and the two-electron reduction of FAD-1, respectively. Both FAD-1 and FAD-2 in holoETF were reduced by excess NADH very rapidly. The reduction of FAD-2 was slowed by replacement of FAD-1 with 8-cyano-FAD indicating that FAD-2 receives electrons from FAD-1 but not from NADH directly. The present results suggest that FAD-2 is the counterpart of the FAD in human ETF, which contains one FAD and one AMP.

Space Shuttle Project

NASA Image and Video Library

1995-10-20

A Great Blue Heron seems oblivious to the tremendous spectacle of light and sound generated by a Shuttle liftoff, as the Space Shuttle Columbia (STS-73) soars skyward from Launch Pad 39B. Columbia's seven member crew's mission included continuing experimentation in the Marshall managed payloads including the United States Microgravity Laboratory 2 (USML-2) and the keel-mounted accelerometer that characterizes the very low frequency acceleration environment of the orbiter payload bay during space flight, known as the Orbital Acceleration Research Experiment (OARE).

Photoelectrochemical NADH Regeneration using Pt-Modified p -GaAs Semiconductor Electrodes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stufano, Paolo; Paris, Aubrey R.; Bocarsly, Andrew

Cofactor regeneration in enzymatic reductions is crucial for the application of enzymes to both biological and energy-related catalysis. Specifically, regenerating NADH from NAD + is of great interest, and using electrochemistry to achieve this end is considered a promising option. Here in this paper, we report the first example of photoelectrochemical NADH regeneration at the illuminated (λ >600 nm), metal-modified p-type semiconductor electrode Pt/p-GaAs. Although bare p-GaAs electrodes produce only enzymatically inactive NAD 2, NADH was produced at the illuminated Pt-modified p-GaAs surface. At low overpotential (–0.75 V vs. Ag/AgCl), Pt/p-GaAs exhibited a seven-fold greater Faradaic efficiency for the formationmore » of NADH than Pt alone, with reduced competition from the hydrogen evolution reaction. Improved Faradaic efficiency and low overpotential suggest the possible utility of Pt/p-GaAs in energy-related NADH-dependent enzymatic processes.« less

Photoelectrochemical NADH Regeneration using Pt-Modified p -GaAs Semiconductor Electrodes

DOE PAGES

Stufano, Paolo; Paris, Aubrey R.; Bocarsly, Andrew

2017-02-22

Cofactor regeneration in enzymatic reductions is crucial for the application of enzymes to both biological and energy-related catalysis. Specifically, regenerating NADH from NAD + is of great interest, and using electrochemistry to achieve this end is considered a promising option. Here in this paper, we report the first example of photoelectrochemical NADH regeneration at the illuminated (λ >600 nm), metal-modified p-type semiconductor electrode Pt/p-GaAs. Although bare p-GaAs electrodes produce only enzymatically inactive NAD 2, NADH was produced at the illuminated Pt-modified p-GaAs surface. At low overpotential (–0.75 V vs. Ag/AgCl), Pt/p-GaAs exhibited a seven-fold greater Faradaic efficiency for the formationmore » of NADH than Pt alone, with reduced competition from the hydrogen evolution reaction. Improved Faradaic efficiency and low overpotential suggest the possible utility of Pt/p-GaAs in energy-related NADH-dependent enzymatic processes.« less

Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia

PubMed Central

Shetty, Pavan K; Galeffi, Francesca; Turner, Dennis A.

2014-01-01

Prolonged hypoxia leads to irreversible loss of neuronal function and metabolic impairment of nicotinamide adenine dinucleotide recycling (between NAD+ and NADH) immediately after reoxygenation, resulting in NADH hyperoxidation. We test whether addition of nicotinamide (to enhance NAD+ levels) or PARP-1 inhibition (to prevent consumption of NAD+) can be effective in improving either loss of neuronal function or hyperoxidation following severe hypoxic injury in hippocampal slices. After severe, prolonged hypoxia (maintained for 3 min after spreading depression) there was hyperoxidation of NADH following reoxygenation, an increased soluble NAD+/NADH ratio, loss of neuronal field excitatory post-synaptic potential (fEPSP) and decreased ATP content. Nicotinamide incubation (5 mM) 2 hr prior to hypoxia significantly increased total NAD(H) content, improved neuronal recovery, enhanced ATP content, and prevented NADH hyperoxidation. The nicotinamide-induced increase in total soluble NAD(H) was more significant in the cytosolic compartment than within mitochondria. Prolonged incubation with PJ-34 (>1hr) led to enhanced baseline NADH fluorescence prior to hypoxia, as well as improved neuronal recovery, NADH hyperoxidation and ATP content on recovery from severe hypoxia and reoxygenation. In this acute model of severe neuronal dysfunction prolonged incubation with either nicotinamide or PJ-34 prior to hypoxia improved recovery of neuronal function, enhanced NADH reduction and ATP content, but neither treatment restored function when administered during or after prolonged hypoxia and reoxygenation. PMID:24184921

Space Shuttle Projects

NASA Image and Video Library

1990-10-06

Launched aboard the Space Shuttle Discovery on October 6, 1990 at 7:47:15 am (EDT), the STS-41 mission consisted of 5 crew members. Included were Richard N. Richards, commander; Robert D. Cabana, pilot; and Bruce E. Melnick, Thomas D. Akers, and William M. Shepherd, all mission specialists. The primary payload for the mission was the European Space Agency (ESA) built Ulysses Space Craft made to explore the polar regions of the Sun. Other main payloads and experiments included the Shuttle Solar Backscatter Ultraviolet (SSBUV) experiment and the INTELSAT Solar Array Coupon (ISAC).

Increased work in cardiac trabeculae causes decreased mitochondrial NADH fluorescence followed by slow recovery.

PubMed Central

Brandes, R; Bers, D M

1996-01-01

The oxidative phosphorylation rate in isolated mitochondria is stimulated by increased [ADP], resulting in decreased [NADH]. In intact hearts, however, increased mechanical work has generally not been shown to cause an increase in [ADP]. Therefore, increased [NADH] has been suggested as an alternative for stimulating the phosphorylation rate. Such a rise in [NADH] could result from stimulation of various substrate dehydrogenases by increased intracellular [Ca2+] (e.g., during increased pacing frequency). We have monitored mitochondrial [NADH] in isolated rat ventricular trabeculae, using a novel fluorescence spectroscopy method where a native fluorescence signal was used to correct for motion artifacts. Work was controlled by increased pacing frequency and assessed using time-averaged force. At low-pacing rates (approximately 0.1 Hz), [NADH] immediately decreased during contraction and then slowly recovered (approximately 5 s) before the next contraction. At higher rates, [NADH] initially decreased by an amount related to pacing rate (i.e., work). However, during prolonged stimulation, [NADH] slowly (approximately 60 s) recovered to a new steady-state level below the initial level. We conclude that 1) during increased work, oxidative phosphorylation is not initially stimulated by increased mitochondrial [NADH]; and 2) increased pacing frequency slowly causes stimulation of NADH production. Images FIGURE 2 FIGURE 4 PMID:8842239

Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida).

PubMed

Cermáková, Petra; Verner, Zdenek; Man, Petr; Lukes, Julius; Horváth, Anton

2007-06-01

NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens, a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity staining. The association of NADH dehydrogenase activities with two distinct multisubunit complexes was revealed in the second dimension performed under denaturing conditions. One subunit present in both complexes cross-reacted with the antibody against the 39 kDa subunit of bovine complex I. Out of several subunits analyzed by MS, one contained a domain characteristic for the LYR family subunit of the NADH:ubiquinone oxidoreductases. Spectrophotometric measurement of the NADH:ubiquinone 10 and NADH:ferricyanide dehydrogenase activities revealed their different sensitivities to rotenone, piericidin, and diphenyl iodonium.

Stabilized NADH as a Countermeasure for Jet Lag

NASA Technical Reports Server (NTRS)

Kay, Gary G.; Viirre, Erik; Clark, Jonathan

2001-01-01

Current remedies for jet lag (phototherapy, melatonin, stimulant, and sedative medications) are limited in efficacy and practicality. The efficacy of a stabilized, sublingual form of reduced nicotin amide adenine dinucleotide (NADH, ENADAlert, Menuco Corp.) as a countermeasure for jet lag was examined. Because NADH increases cellular production of ATP and facilitates dopamine synthesis, it may counteract the effects of jet lag on cognitive functioning and sleepiness. Thirty-five healthy, employed subjects participated in this double-blind, placebo-controlled study. Training and baseline testing were conducted on the West Coast before subjects flew overnight to the East Coast, where they would experience a 3-hour time difference. Upon arrival, individuals were randomly assigned to receive either 20 mg of sublingual stabilized ADH (n=18) or identical placebo tablets (n=17). All participants completed computer-administered tests (including CogScreen7) to assess changes in cognitive functioning, mood, and sleepiness in the morning and afternoon. Jet lag resulted in increased sleepiness for over half the participants and deterioration of cognitive functioning for approximately one third. The morning following the flight, subjects experienced lapses of attention in addition to disruptions in working memory, divided attention, and visual perceptual speed. Individuals who received NADH performed significantly better on 5 of 8 cognitive and psychomotor test measures (P less than or equal to 0.5) and showed a trend for better performance on the other three measures (P less than or equal to .l0). Subjects also reported less sleepiness compared with those who received placebo. No adverse effects were observed with NADH treatment. Stabilized NADH significantly reduced jet lag-induced disruptions of cognitive functioning, was easily administered, and was found to have no adverse side effects.

Pharmacological Stimulation of NADH Oxidation Ameliorates Obesity and Related Phenotypes in Mice

PubMed Central

Hwang, Jung Hwan; Kim, Dong Wook; Jo, Eun Jin; Kim, Yong Kyung; Jo, Young Suk; Park, Ji Hoon; Yoo, Sang Ku; Park, Myung Kyu; Kwak, Tae Hwan; Kho, Young Lim; Han, Jin; Choi, Hueng-Sik; Lee, Sang-Hee; Kim, Jin Man; Lee, InKyu; Kyung, Taeyoon; Jang, Cholsoon; Chung, Jongkyeong; Kweon, Gi Ryang; Shong, Minho

2009-01-01

OBJECTIVE Nicotinamide adenine dinucleotides (NAD+ and NADH) play a crucial role in cellular energy metabolism, and a dysregulated NAD+-to-NADH ratio is implicated in metabolic syndrome. However, it is still unknown whether a modulating intracellular NAD+-to-NADH ratio is beneficial in treating metabolic syndrome. We tried to determine whether pharmacological stimulation of NADH oxidation provides therapeutic effects in rodent models of metabolic syndrome. RESEARCH DESIGN AND METHODS We used β-lapachone (βL), a natural substrate of NADH:quinone oxidoreductase 1 (NQO1), to stimulate NADH oxidation. The βL-induced pharmacological effect on cellular energy metabolism was evaluated in cells derived from NQO1-deficient mice. In vivo therapeutic effects of βL on metabolic syndrome were examined in diet-induced obesity (DIO) and ob/ob mice. RESULTS NQO1-dependent NADH oxidation by βL strongly provoked mitochondrial fatty acid oxidation in vitro and in vivo. These effects were accompanied by activation of AMP-activated protein kinase and carnitine palmitoyltransferase and suppression of acetyl-coenzyme A (CoA) carboxylase activity. Consistently, systemic βL administration in rodent models of metabolic syndrome dramatically ameliorated their key symptoms such as increased adiposity, glucose intolerance, dyslipidemia, and fatty liver. The treated mice also showed higher expressions of the genes related to mitochondrial energy metabolism (PPARγ coactivator-1α, nuclear respiratory factor-1) and caloric restriction (Sirt1) consistent with the increased mitochondrial biogenesis and energy expenditure. CONCLUSIONS Pharmacological activation of NADH oxidation by NQO1 resolves obesity and related phenotypes in mice, opening the possibility that it may provide the basis for a new therapy for the treatment of metabolic syndrome. PMID:19136651

Columnar alterations of NADH fluorescence during hypoxia-ischemia in immature rat brain.

PubMed

Welsh, F A; Vannucci, R C; Brierley, J B

1982-01-01

Cerebral hypoxia-ischemia was produced in 7-day postnatal rats by unilateral carotid artery ligation combined with systemic hypoxia (8% O2). Levels of high energy phosphates, which were only slightly altered in the contralateral hemisphere, were nearly depleted in the ipsilateral hemisphere during the 3-h hypoxic insult. With hypoxia of between 1 and 3 hours' duration, columnar alterations of cortical NADH fluorescence occurred in the same location and regional pattern as did histologic damage demonstrated previously (Rice et al., 1981). In regions exhibiting columns of NADH fluorescence, there was no evidence of a columnar reduction of high energy phosphates as levels of ATP and phosphocreatine were nearly zero. Recovery from 3 h of hypoxia was accompanied by partial and regionally heterogeneous restoration of ATP within the ipsilateral hemisphere. Columnar variations of NADH fluorescence were not detected in the recovery period; rather, regions with impaired restitution of high energy phosphates exhibited NADH fluorescence that was diminished diffusely compared to the contralateral hemisphere. The correlation between depressed NADH fluorescence and depleted ATP, present as cortical columns during hypoxia and as larger regions during recovery, suggests that decreased formation of NADH may be limiting the resynthesis of high energy phosphates.

[Membrane lipids and electron transfer. Effects of four detergents on NADH-ferricyanide reductase and NADH-cytochrome c reductase activities of potato tuber microsomes].

PubMed

Jolliot, A; Mazliak, P

1977-10-17

The NADH-ferricyanure reductase activity of Potato microsomes is stimulated by non ionic detergents (Triton X100 and Tween80) and is partially inhibited by ionic detergents (sodium-cholate and deoxycholate). All these four detergents progressively decreased the NADH-cytochrome c reductase in the following order: sodium deoxycholate greater than Triton X100 greater than sodium cholate greater than Tween80.

Analysis of sleep on Shuttle missions

NASA Technical Reports Server (NTRS)

Santy, Patricia A.; Kapanka, Heidi; Davis, Jeffrey R.; Stewart, Donald F.

1988-01-01

The sleep patterns of 58 Space Shuttle crew members are analyzed statistically on the basis of debriefing forms filled out within 3 days postflight. The data are compiled in a table, and photographs of typical sleep conditions on the Shuttle are provided. It is found that sleep disruption is relatively common on Shuttle missions, especially on the first and last days. Sleep medication was used by 19.4 percent of crew on single-shift flights and 50 percent of crew on dual-shift flights.

Space Shuttle Projects

NASA Image and Video Library

1990-11-16

The 5 member crew of the STS-41 mission included (left to right): Bruce E. Melnick, mission specialist 2; Robert D. Cabana, pilot; Thomas D. Akers, mission specialist 3; Richard N. Richards, commander; and William M. Shepherd, mission specialist 1. Launched aboard the Space Shuttle Discovery on October 6, 1990 at 7:47:15 am (EDT), the primary payload for the mission was the ESA built Ulysses Space Craft made to explore the polar regions of the Sun. Other main payloads and experiments included the Shuttle Solar Backscatter Ultraviolet (SSBUV) experiment and the INTELSAT Solar Array Coupon (ISAC).

Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts

NASA Astrophysics Data System (ADS)

Moreno, Angel; Kuzmiak-Glancy, Sarah; Jaimes, Rafael; Kay, Matthew W.

2017-03-01

Reduction of NAD+ by dehydrogenase enzymes to form NADH is a key component of cellular metabolism. In cellular preparations and isolated mitochondria suspensions, enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an effective approach for measuring the rate of NADH production to assess dehydrogenase enzyme activity. Our objective was to demonstrate how dehydrogenase activity could be assessed within the myocardium of perfused hearts using NADH ED-FRAP. This was accomplished using a combination of high intensity UV pulses to photobleach epicardial NADH. Replenishment of epicardial NADH fluorescence was then imaged using low intensity UV illumination. NADH ED-FRAP parameters were optimized to deliver 23.8 mJ of photobleaching light energy at a pulse width of 6 msec and a duty cycle of 50%. These parameters provided repeatable measurements of NADH production rate during multiple metabolic perturbations, including changes in perfusate temperature, electromechanical uncoupling, and acute ischemia/reperfusion injury. NADH production rate was significantly higher in every perturbation where the energy demand was either higher or uncompromised. We also found that NADH production rate remained significantly impaired after 10 min of reperfusion after global ischemia. Overall, our results indicate that myocardial NADH ED-FRAP is a useful optical non-destructive approach for assessing dehydrogenase activity.

Enhancement of succinate yield by manipulating NADH/NAD+ ratio and ATP generation.

PubMed

Li, Jiaojiao; Li, Yikui; Cui, Zhiyong; Liang, Quanfeng; Qi, Qingsheng

2017-04-01

We previously engineered Escherichia coli YL104 to efficiently produce succinate from glucose. In this study, we investigated the relationships between the NADH/NAD + ratio, ATP level, and overall yield of succinate production by using glucose as the carbon source in YL104. First, the use of sole NADH dehydrogenases increased the overall yield of succinate by 7% and substantially decreased the NADH/NAD + ratio. Second, the soluble fumarate reductase from Saccharomyces cerevisiae was overexpressed to manipulate the anaerobic NADH/NAD + ratio and ATP level. Third, another strategy for reducing the ATP level was applied by introducing ATP futile cycling for improving succinate production. Finally, a combination of these methods exerted a synergistic effect on improving the overall yield of succinate, which was 39% higher than that of the previously engineered strain YL104. The study results indicated that regulation of the NADH/NAD + ratio and ATP level is an efficient strategy for succinate production.

Metabolic control by sirtuins and other enzymes that sense NAD+, NADH, or their ratio.

PubMed

Anderson, Kristin A; Madsen, Andreas S; Olsen, Christian A; Hirschey, Matthew D

2017-12-01

NAD + is a dinucleotide cofactor with the potential to accept electrons in a variety of cellular reduction-oxidation (redox) reactions. In its reduced form, NADH is a ubiquitous cellular electron donor. NAD + , NADH, and the NAD + /NADH ratio have long been known to control the activity of several oxidoreductase enzymes. More recently, enzymes outside those participating directly in redox control have been identified that sense these dinucleotides, including the sirtuin family of NAD + -dependent protein deacylases. In this review, we highlight examples of non-redox enzymes that are controlled by NAD + , NADH, or NAD + /NADH. In particular, we focus on the sirtuin family and assess the current evidence that the sirtuin enzymes sense these dinucleotides and discuss the biological conditions under which this might occur; we conclude that sirtuins sense NAD + , but neither NADH nor the ratio. Finally, we identify future studies that might be informative to further interrogate physiological and pathophysiological changes in NAD + and NADH, as well as enzymes like sirtuins that sense and respond to redox changes in the cell. Copyright © 2017 Elsevier B.V. All rights reserved.

Cofactor engineering to regulate NAD+/NADH ratio with its application to phytosterols biotransformation.

PubMed

Su, Liqiu; Shen, Yanbing; Zhang, Wenkai; Gao, Tian; Shang, Zhihua; Wang, Min

2017-10-30

Cofactor engineering is involved in the modification of enzymes related to nicotinamide adenine dinucleotides (NADH and NAD + ) metabolism, which results in a significantly altered spectrum of metabolic products. Cofactor engineering plays an important role in metabolic engineering but is rarely reported in the sterols biotransformation process owing to its use of multi-catabolic enzymes, which promote multiple consecutive reactions. Androst-4-ene-3, 17-dione (AD) and androst-1, 4-diene-3, 17-dione (ADD) are important steroid medicine intermediates that are obtained via the nucleus oxidation and the side chain degradation of phytosterols by Mycobacterium. Given that the biotransformation from phytosterols to AD (D) is supposed to be a NAD + -dependent process, this work utilized cofactor engineering in Mycobacterium neoaurum and investigated the effect on cofactor and phytosterols metabolism. Through the addition of the coenzyme precursor of nicotinic acid in the phytosterols fermentation system, the intracellular NAD + /NADH ratio and the AD (D) production of M. neoaurum TCCC 11978 (MNR M3) were higher than in the control. Moreover, the NADH: flavin oxidoreductase was identified and was supposed to exert a positive effect on cofactor regulation and phytosterols metabolism pathways via comparative proteomic profiling of MNR cultured with and without phytosterols. In addition, the NADH: flavin oxidoreductase and a water-forming NADH oxidase from Lactobacillus brevis, were successfully overexpressed and heterologously expressed in MNR M3 to improve the intracellular ratio of NAD + /NADH. After 96 h of cultivation, the expression of these two enzymes in MNR M3 resulted in the decrease in intracellular NADH level (by 51 and 67%, respectively) and the increase in NAD + /NADH ratio (by 113 and 192%, respectively). Phytosterols bioconversion revealed that the conversion ratio of engineered stains was ultimately improved by 58 and 147%, respectively. The highest AD (D

Multiphoton fluorescence imaging of NADH to quantify metabolic changes in epileptic tissue in vitro

NASA Astrophysics Data System (ADS)

Chia, Thomas H.; Zinter, Joseph; Spencer, Dennis D.; Williamson, Anne; Levene, Michael J.

2007-02-01

A powerful advantage of multiphoton microscopy is its ability to image endogenous fluorophores such as the ubiquitous coenzyme NADH in discrete cellular populations. NADH is integral in both oxidative and non-oxidative cellular metabolism. NADH loses fluorescence upon oxidation to NAD +; thus changes in NADH fluorescence can be used to monitor metabolism. Recent studies have suggested that hypo metabolic astrocytes play an important role in cases of temporal lobe epilepsy (TLE). Current theories suggest this may be due to defective and/or a reduced number of mitochondria or dysfunction of the neuronal-astrocytic metabolic coupling. Measuring NADH fluorescence changes following chemical stimulation enables the quantification of the cellular distribution of metabolic anomalies in epileptic brain tissue compared to healthy tissue. We present what we believe to be the first multiphoton microscopy images of NADH from the human brain. We also present images of NADH fluorescence from the hippocampus of the kainate-treated rat TLE model. In some experiments, human and rat astrocytes were selectively labeled with the fluorescent dye sulforhodamine 101 (SR101). Our results demonstrate that multiphoton microscopy is a powerful tool for assaying the metabolic pathologies associated with temporal lobe epilepsy in humans and in rodent models.

STS-92 - Towing of Shuttle Discovery and Boeing 747 Shuttle Carrier Aircraft (SCA)

NASA Technical Reports Server (NTRS)

2000-01-01

The Space Shuttle Discovery sits atop one of NASA's modified Boeing 747 Shuttle Carrier Aircraft as the unusual piggyback duo is towed along a taxiway at NASA's Dryden Flight Research Center at Edwards, California. The Discovery was ferried from NASA Dryden to NASA's Kennedy Space Center in Florida on November 2, 2000, after extensive pre-ferry servicing and preparations. STS-92 was the 100th mission since the fleet of four Space Shuttles began flying in 1981. (Due to schedule changes, missions are not always launched in the order that was originally planned.) The almost 13-day mission, the 46th Shuttle mission to land at Edwards, was the last construction mission for the International Space Station prior to the first scientists taking up residency in the orbiting space laboratory the following month. The seven-member crew on STS-92 included mission specialists Koichi Wakata, Michael Lopez-Alegria, Jeff Wisoff, Bill McArthur and Leroy Chiao, pilot Pam Melroy and mission commander Brian Duffy.

Regulation of NADH/CoQ oxidoreductase: do phosphorylation events affect activity?

PubMed

Maj, Mary C; Raha, Sandeep; Myint, Tomoko; Robinson, Brian H

2004-01-01

We had previously suggested that phosphorylation of proteins by mitochondrial kinases regulate the activity of NADH/CoQ oxidoreductase. Initial data showed that pyruvate dehydrogenase kinase (PDK) and cAMP-dependent protein kinase A (PKA) phosphorylate mitochondrial membrane proteins. Upon phosphorylation with crude PDK, mitochondria appeared to be deficient in NADH/cytochrome c reductase activity associated with increased superoxide production. Conversely, phosphorylation by PKA resulted in increased NADH/cytochrome c reductase activity and decreased superoxide formation. Current data confirms PKA involvement in regulating Complex I activity through phosphorylation of an 18 kDa subunit. Beef heart NADH/ cytochrome c reductase activity increases to 150% of control upon incubation with PKA and ATP-gamma-S. We have cloned the four human isoforms of PDK and purified beef heart Complex I. Incubation of mitochondria with PDK isoforms and ATP did not alter Complex I activity or superoxide production. Radiolabeling of mitochondria and purified Complex I with PDK failed to reveal phosphorylated proteins.

Stimulation of NADH-dependent microsomal DNA strand cleavage by rifamycin SV.

PubMed

Kukiełka, E; Cederbaum, A I

1995-04-15

Rifamycin SV is an antibiotic anti-bacterial agent used in the treatment of tuberculosis. This drug can autoxidize, especially in the presence of metals, and generate reactive oxygen species. A previous study indicated that rifamycin SV can increase NADH-dependent microsomal production of reactive oxygen species. The current study evaluated the ability of rifamycin SV to interact with iron and increase microsomal production of hydroxyl radical, as detected by conversion of supercoiled plasmid DNA into the relaxed open circular state. The plasmid used was pBluescript II KS(-), and the forms of DNA were separated by agarose-gel electrophoresis. Incubation of rat liver microsomes with plasmid plus NADH plus ferric-ATP caused DNA strand cleavage. The addition of rifamycin SV produced a time- and concentration-dependent increase in DNA-strand cleavage. No stimulation by rifamycin SV occurred in the absence of microsomes, NADH or ferric-ATP. Stimulation occurred with other ferric complexes besides ferric-ATP, e.g. ferric-histidine, ferric-citrate, ferric-EDTA, and ferric-(NH4)2SO4. Rifamycin SV did not significantly increase the high rates of DNA strand cleavage found with NADPH as the microsomal reductant. The stimulation of NADH-dependent microsomal DNA strand cleavage was completely blocked by catalase, superoxide dismutase, GSH and a variety of hydroxyl-radical-scavenging agents, but not by anti-oxidants that prevent microsomal lipid peroxidation. Redox cycling agents, such as menadione and paraquat, in contrast with rifamycin SV, stimulated the NADPH-dependent reaction; menadione and rifamycin SV were superior to paraquat in stimulating the NADH-dependent reaction. These results indicate that rifamycin SV can, in the presence of an iron catalyst, increase microsomal production of reactive oxygen species which can cause DNA-strand cleavage. In contrast with other redox cycling agents, the stimulation by rifamycin SV is more pronounced with NADH than with NADPH as the

Engineering of a functional human NADH-dependent cytochrome P450 system

PubMed Central

Döhr, Olaf; Paine, Mark J. I.; Friedberg, Thomas; Roberts, Gordon C. K.; Wolf, C. Roland

2001-01-01

A functional human NADH-dependent cytochrome P450 system has been developed by altering the cofactor preference of human NADPH cytochrome P450 reductase (CPR), the redox partner for P450s. This has been achieved by a single amino acid change of the conserved aromatic amino acid Trp-676, which covers the re-side of the FAD isoalloxazine ring in the nicotinamide-binding site. Of the mutations made, the substitution of Trp-676 with alanine (W676A) resulted in a functional NADH-dependent enzyme, which catalyzed the reduction of cytochrome c and ferricyanide as well as facilitated the metabolism of 7-ethoxyresorufin by CYP1A2. Kinetic analysis measuring cytochrome c activity revealed that the NADH-dependent kcat of W676A is equivalent (90%) to the NADPH-dependent kcat of the wild-type enzyme, with W676A having an approximately 1,000-fold higher specificity for NADH. The apparent KMNADPH and KMNADH values of W676A are 80- and 150-fold decreased, respectively. In accordance with structural data, which show a bipartite binding mode of NADPH, substitution of Trp-676 does not affect 2′-AMP binding as seen by the inhibition of both wild-type CPR and the W676A mutant. Furthermore, NADPH was a potent inhibitor of the W676A NADH-dependent cytochrome c reduction and CYP1A2 activity. Overall, the results show that Trp-676 of human CPR plays a major role in cofactor discrimination, and substitution of this conserved aromatic residue with alanine results in an efficient NADH-dependent cytochrome P450 system. PMID:11136248

Enzymatic properties of the membrane-bound NADH oxidase system in the aerobic respiratory chain of Bacillus cereus.

PubMed

Kim, Man Suk; Kim, Young Jae

2004-11-30

Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase exhibited an apparent K(m) value of approximately 65 microM for NADH. The maximum activity of the NADH oxidase was obtained at about pH 8.5 in the presence of 0.1 M KCl (or NaCl). Respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) inhibited the activity of the NADH oxidase by about 90% at a concentration of 40 microM. Interestingly, rotenone and capsaicin inhibited the activity of the NADH oxidase by about 60% at a concentration of 40 microM and the activity was also highly sensitive to Ag(+).

Space Shuttle Projects

NASA Image and Video Library

1991-04-05

Aboard the Space Shuttle Atlantis, the STS-37 mission launched April 5, 1991 from launch pad 39B at the Kennedy Space Center in Florida, and landed back on Earth April 11, 1991. The 39th shuttle mission included crew members: Steven R. Nagel, commander; Kenneth D. Cameron, pilot; Jerry L,. Ross, mission specialist 1; Jay Apt, mission specialist 2; and Linda M. Godwin, mission specialist 3. The primary payload for the mission was the Gamma Ray Observatory (GRO). The GRO included the Burst and Transient Experiment (BATSE); the Imaging Compton Telescope (COMPTEL); the Energetic Gamma Ray Experiment Telescope (EGRET); and the Oriented Scintillation Spectrometer Experiment (OSSEE). Secondary payloads included Crew and Equipment Translation Aids (CETA); the Ascent Particle Monitor (APM); the Shuttle Amateur Radio Experiment II (SAREXII), the Protein Crystal Growth (PCG); the Bioserve Instrumentation Technology Associates Materials Dispersion Apparatus (BIMDA); Radiation Monitoring Equipment III (RMEIII); and Air Force Maui Optical Site (AMOS).

Space Shuttle Projects

NASA Image and Video Library

1995-06-07

Designed by the mission crew members, the patch for STS-69 symbolizes the multifaceted nature of the flight's mission. The primary payload, the Wake Shield Facility (WSF), is represented in the center by the astronaut emblem against a flat disk. The astronaut emblem also signifies the importance of human beings in space exploration, reflected by the planned space walk to practice for International Space Station (ISS) activities and to evaluate space suit design modifications. The two stylized Space Shuttles highlight the ascent and entry phases of the mission. Along with the two spiral plumes, the stylized Space Shuttles symbolize a NASA first, the deployment and recovery on the same mission of two spacecraft (both the Wake Shield Facility and the Spartan). The constellations Canis Major and Canis Minor represent the astronomy objectives of the Spartan and International Extreme Ultraviolet Hitchhiker (IEH) payload. The two constellations also symbolize the talents and dedication of the support personnel who make Space Shuttle missions possible.

Space Shuttle Projects

NASA Image and Video Library

1995-05-27

The crew patch of STS-72 depicts the Space Shuttle Endeavour and some of the payloads on the flight. The Japanese satellite, Space Flyer Unit (SFU) is shown in a free-flying configuration with the solar array panels deployed. The inner gold border of the patch represents the SFU's distinct octagonal shape. Endeavour’s rendezvous with and retrieval of SFU at an altitude of approximately 250 nautical miles. The Office of Aeronautics and Space Technology's (OAST) flyer satellite is shown just after release from the Remote Manipulator System (RMS). The OAST satellite was deployed at an altitude of 165 nautical miles. The payload bay contains equipment for the secondary payloads - the Shuttle Laser Altimeter (SLA) and the Shuttle Solar Backscatter Ultraviolet Instrument (SSBUV). There were two space walks planned to test hardware for assembly of the International Space Station. The stars represent the hometowns of the crew members in the United States and Japan.

Evaluation of functioning of mitochondrial electron transport chain with NADH and FAD autofluorescence

PubMed

Danylovych, H V

2016-01-01

We prove the feasibility of evaluation of mitochondrial electron transport chain function in isolated mitochondria of smooth muscle cells of rats from uterus using fluorescence of NADH and FAD coenzymes. We found the inversely directed changes in FAD and NADH fluorescence intensity under normal functioning of mitochondrial electron transport chain. The targeted effect of inhibitors of complex I, III and IV changed fluorescence of adenine nucleotides. Rotenone (5 μM) induced rapid increase in NADH fluorescence due to inhibition of complex I, without changing in dynamics of FAD fluorescence increase. Antimycin A, a complex III inhibitor, in concentration of 1 μg/ml caused sharp increase in NADH fluorescence and moderate increase in FAD fluorescence in comparison to control. NaN3 (5 mM), a complex IV inhibitor, and CCCP (10 μM), a protonophore, caused decrease in NADH and FAD fluorescence. Moreover, all the inhibitors caused mitochondria swelling. NO donors, e.g. 0.1 mM sodium nitroprusside and sodium nitrite similarly to the effects of sodium azide. Energy-dependent Ca2+ accumulation in mitochondrial matrix (in presence of oxidation substrates and Mg-ATP2- complex) is associated with pronounced drop in NADH and FAD fluorescence followed by increased fluorescence of adenine nucleotides, which may be primarily due to Ca2+- dependent activation of dehydrogenases of citric acid cycle. Therefore, the fluorescent signal of FAD and NADH indicates changes in oxidation state of these nucleotides in isolated mitochondria, which may be used to assay the potential of effectors of electron transport chain.

Space Shuttle Projects

NASA Image and Video Library

1989-11-22

On November 22, 1989, at 7:23:30pm (EST), five astronauts were launched into space aboard the Space Shuttle Orbiter Discovery for the 5th Department of Defense (DOD) mission, STS-33. Crew members included Frederick D. Gregory, commander; John E. Blaha, pilot; and mission specialists Kathryn C. Thornton, Manley L. (Sonny) Carter, and F. Story Musgrave.

Visible light-driven NADH regeneration sensitized by proflavine for biocatalysis.

PubMed

Nam, Dong Heon; Park, Chan Beum

2012-06-18

Harvest time: Proflavine drives the reduction of NAD(+) in the presence of a Rh-based electron mediator. Photoregenerated NADH was enzymatically active for oxidation by NADH-dependent L-glutamate dehydrogenase for the synthesis of L-glutamate. This work suggests that proflavine has the potential to become an efficient light-harvesting component in biocatalytic photosynthesis driven by solar energy. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence.

PubMed

Li, Dong; Zheng, Wei; Qu, Jianan Y

2008-10-15

A time-resolved spectroscopic imaging system is built to study the fluorescence characteristics of nicotinamide adenine dinucleotide (NADH), an important metabolic coenzyme and endogenous fluorophore in cells. The system provides a unique approach to measure fluorescence signals in different cellular organelles and cytoplasm. The ratios of free over protein-bound NADH signals in cytosol and nucleus are slightly higher than those in mitochondria. The mitochondrial fluorescence contributes about 70% of overall cellular fluorescence and is not a completely dominant signal. Furthermore, NADH signals in mitochondria, cytosol, and the nucleus respond to the changes of cellular activity differently, suggesting that cytosolic and nuclear fluorescence may complicate the well-known relationship between mitochondrial fluorescence and cellular metabolism.

Design of a space shuttle structural dynamics model

NASA Technical Reports Server (NTRS)

1972-01-01

A 1/8 scale structural dynamics model of a parallel burn space shuttle has been designed. Basic objectives were to represent the significant low frequency structural dynamic characteristics while keeping the fabrication costs low. The model was derived from the proposed Grumman Design 619 space shuttle. The design includes an orbiter, two solid rocket motors (SRM) and an external tank (ET). The ET consists of a monocoque LO2 tank an interbank skirt with three frames to accept SRM attachment members, an LH2 tank with 10 frames of which 3 provide for orbiter attachment members, and an aft skirt with on frame to provide for aft SRM attachment members. The frames designed for the SRM attachments are fitted with transverse struts to take symmetric loads.

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis cuts its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Fluorophores advanced glycation end products (AGEs)-to-NADH ratio is predictor for diabetic chronic kidney and cardiovascular disease.

PubMed

Ciobanu, Dana M; Olar, Loredana E; Stefan, Razvan; Veresiu, Ioan A; Bala, Cornelia G; Mircea, Petru A; Roman, Gabriela

2015-01-01

An imbalance in advanced glycation end products (AGEs) and NADH formation has been associated with diabetic chronic kidney disease (CKD) and cardiovascular disease (CVD). No data have been reported on simultaneous measurement of AGEs and NADH in type 2 diabetes (T2DM) patients. We aimed to compare AGEs, NADH and the AGEs-to-NADH ratio in T2DM and controls, and to assess its relationship with diabetic CKD and CVD. In this cross-sectional study, we measured serum AGEs (370/435nm) and NADH (370/460nm) in T2DM patients (n=63) and controls (n=25) using fluorescence spectroscopy. The AGEs-to-NADH ratio was analyzed according to diabetic CKD and CVD. We found significantly higher AGEs-to-NADH ratio in T2DM compared to controls. The AGEs-to-NADH ratio was significantly associated with triglycerides, blood glucose, HDL-cholesterol, estimated glomerular filtration rate. The AGEs-to-NADH ratio was a significant predictor for the presence of diabetic CKD and CVD when using ROC curves. Multivariate analysis showed that triglycerides and the presence of T2DM were predictors for the AGEs-to-NADH ratio. These findings suggest that the fluorophores AGEs-to-NADH ratio could be a new biomarker for the presence of diabetic CKD and CVD. Copyright © 2015 Elsevier Inc. All rights reserved.

Space Shuttle mission: STS-67

NASA Technical Reports Server (NTRS)

1995-01-01

The Space Shuttle Endeavor, scheduled to launch March 2, 1995 from NASA's Kennedy Space Center, will conduct NASA's longest Shuttle flight prior to date. The mission, designated STS-67, has a number of experiments and payloads, which the crew, commanded by Stephen S. Oswald, will have to oversee. This NASA press kit for the mission contains a general background (general press release, media services information, quick-look facts page, shuttle abort modes, summary timeline, payload and vehicle weights, orbital summary, and crew responsibilities); cargo bay payloads and activities (Astro 2, Get Away Special Experiments); in-cabin payloads (Commercial Minimum Descent Altitude Instrumentation Technology Associates Experiments, protein crystal growth experiments, Middeck Active Control Experiment, and Shuttle Amateur Radio Experiment); and the STS-67 crew biographies. The payloads and experiments are described and summarized to give an overview of the goals, objectives, apparatuses, procedures, sponsoring parties, and the assigned crew members to carry out the tasks.

Involvement of NADH Oxidase in Competition and Endocarditis Virulence in Streptococcus sanguinis

PubMed Central

Ge, Xiuchun; Yu, Yang; Zhang, Min; Chen, Lei; Chen, Weihua; Elrami, Fadi; Kong, Fanxiang; Kitten, Todd

2016-01-01

Here, we report for the first time that the Streptococcus sanguinis nox gene encoding NADH oxidase is involved in both competition with Streptococcus mutans and virulence for infective endocarditis. An S. sanguinis nox mutant was found to fail to inhibit the growth of Streptococcus mutans under microaerobic conditions. In the presence of oxygen, the recombinant Nox protein of S. sanguinis could reduce oxygen to water and oxidize NADH to NAD+. The oxidation of NADH to NAD+ was diminished in the nox mutant. The nox mutant exhibited decreased levels of extracellular H2O2; however, the intracellular level of H2O2 in the mutant was increased. Furthermore, the virulence of the nox mutant was attenuated in a rabbit endocarditis model. The nox mutant also was shown to be more sensitive to blood killing, oxidative and acid stresses, and reduced growth in serum. Thus, NADH oxidase contributes to multiple phenotypes related to competitiveness in the oral cavity and systemic virulence. PMID:26930704

Involvement of NADH Oxidase in Competition and Endocarditis Virulence in Streptococcus sanguinis.

PubMed

Ge, Xiuchun; Yu, Yang; Zhang, Min; Chen, Lei; Chen, Weihua; Elrami, Fadi; Kong, Fanxiang; Kitten, Todd; Xu, Ping

2016-05-01

Here, we report for the first time that the Streptococcus sanguinis nox gene encoding NADH oxidase is involved in both competition with Streptococcus mutans and virulence for infective endocarditis. An S. sanguinis nox mutant was found to fail to inhibit the growth of Streptococcus mutans under microaerobic conditions. In the presence of oxygen, the recombinant Nox protein of S. sanguinis could reduce oxygen to water and oxidize NADH to NAD(+) The oxidation of NADH to NAD(+) was diminished in the nox mutant. The nox mutant exhibited decreased levels of extracellular H2O2; however, the intracellular level of H2O2 in the mutant was increased. Furthermore, the virulence of the nox mutant was attenuated in a rabbit endocarditis model. The nox mutant also was shown to be more sensitive to blood killing, oxidative and acid stresses, and reduced growth in serum. Thus, NADH oxidase contributes to multiple phenotypes related to competitiveness in the oral cavity and systemic virulence. Copyright © 2016 Ge et al.

Space Shuttle Projects

NASA Image and Video Library

1993-05-01

Designed by members of the flight crew, the STS-58 insignia depicts the Space Shuttle Columbia with a Spacelab module in its payload bay in orbit around Earth. The Spacelab and the lettering Spacelab Life Sciences ll highlight the primary mission of the second Space Shuttle flight dedicated to life sciences research. An Extended Duration Orbiter (EDO) support pallet is shown in the aft payload bay, stressing the scheduled two-week duration of the longest Space Shuttle mission to date. The hexagonal shape of the patch depicts the carbon ring, a molecule common to all living organisms. Encircling the inner border of the patch is the double helix of DNA, representing the genetic basis of life. Its yellow background represents the sun, energy source for all life on Earth. Both medical and veterinary caducei are shown to represent the STS- 58 life sciences experiments. The position of the spacecraft in orbit about Earth with the United States in the background symbolizes the ongoing support of the American people for scientific research intended to benefit all mankind.

Fed-batch control based upon the measurement of intracellular NADH

NASA Technical Reports Server (NTRS)

Armiger, W. B.; Lee, J. F.; Montalvo, L. M.; Forro, J. R.

1987-01-01

A series of experiments demonstrating that on-line measurements of intracellular NADH by culture fluorescence can be used to monitor and control the fermentation process are described. A distinct advantage of intercellular NADH measurements over other monitoring techniques such as pH and dissolved oxygen is that it directly measures real time events occurring within the cell rather than changes in the environment. When coupled with other measurement parameters, it can provide a finer degree of sophistication in process control.

Space Shuttle Projects

NASA Image and Video Library

1994-09-16

Astronaut Mark Lee floats freely as he tests the new backpack called the Simplified Aid for EVA Rescue (SAFER) system. SAFER is designed for use in the event a crew member becomes untethered while conducting an EVA. The STS-64 mission marked the first untethered U.S. EVA in 10 years, and was launched on September 9, 1994, aboard the Space Shuttle Orbiter Discovery.

NADH oxidase activity of rat and human liver xanthine oxidoreductase: potential role in superoxide production.

PubMed

Maia, Luisa; Duarte, Rui O; Ponces-Freire, Ana; Moura, José J G; Mira, Lurdes

2007-08-01

To characterise the NADH oxidase activity of both xanthine dehydrogenase (XD) and xanthine oxidase (XO) forms of rat liver xanthine oxidoreductase (XOR) and to evaluate the potential role of this mammalian enzyme as an O2*- source, kinetics and electron paramagnetic resonance (EPR) spectroscopic studies were performed. A steady-state kinetics study of XD showed that it catalyses NADH oxidation, leading to the formation of one O2*- molecule and half a H(2)O(2) molecule per NADH molecule, at rates 3 times those observed for XO (29.2 +/- 1.6 and 9.38 +/- 0.31 min(-1), respectively). EPR spectra of NADH-reduced XD and XO were qualitatively similar, but they were quantitatively quite different. While NADH efficiently reduced XD, only a great excess of NADH reduced XO. In agreement with reductive titration data, the XD specificity constant for NADH (8.73 +/- 1.36 microM(-1) min(-1)) was found to be higher than that of the XO specificity constant (1.07 +/- 0.09 microM(-1) min(-1)). It was confirmed that, for the reducing substrate xanthine, rat liver XD is also a better O2*- source than XO. These data show that the dehydrogenase form of liver XOR is, thus, intrinsically more efficient at generating O2*- than the oxidase form, independently of the reducing substrate. Most importantly, for comparative purposes, human liver XO activity towards NADH oxidation was also studied, and the kinetics parameters obtained were found to be very similar to those of the XO form of rat liver XOR, foreseeing potential applications of rat liver XOR as a model of the human liver enzyme.

NADH fluorescence lifetime analysis of the effect of magnesium ions on ALDH2

USDA-ARS?s Scientific Manuscript database

ALDH2 catalyzes oxidation of toxic aldehydes to their corresponding carboxylic acids. Magnesium ions influence enzyme activity in part by increasing NADH binding affinity. Traditional fluorescence measurements have monitored the blue shift of the NADH fluorescence spectrum to elucidate the extent of...

NADH fluorescence lifetime analysis of the effect of magnesium ions on ALDH2

USDA-ARS?s Scientific Manuscript database

Aldehyde dehydrogenase 2 (ALDH2) catalyzes oxidation of toxic aldehydes to carboxylic acids. Physiologic levels of Mg2+ ions influence enzyme activity in part by increasing NADH binding affinity. Traditional fluorescence measurements monitor the blue shift of the NADH fluorescence spectrum to study ...

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis zooms into the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site witness space shuttle Atlantis cut its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis races to orbit over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site watch as space shuttle Atlantis springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Like a phoenix rising from the flames, space shuttle Atlantis takes flight from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site have front-row seats as space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - An exhaust cloud begins to form around space shuttle Atlantis as it springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit:Jim Grossmann

Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae.

PubMed

Hou, Jin; Vemuri, Goutham N; Bao, Xiaoming; Olsson, Lisbeth

2009-04-01

During growth of Saccharomyces cerevisiae on glucose, the redox cofactors NADH and NADPH are predominantly involved in catabolism and biosynthesis, respectively. A deviation from the optimal level of these cofactors often results in major changes in the substrate uptake and biomass formation. However, the metabolism of xylose by recombinant S. cerevisiae carrying xylose reductase and xylitol dehydrogenase from the fungal pathway requires both NADH and NADPH and creates cofactor imbalance during growth on xylose. As one possible solution to overcoming this imbalance, the effect of overexpressing the native NADH kinase (encoded by the POS5 gene) in xylose-consuming recombinant S. cerevisiae directed either into the cytosol or to the mitochondria was evaluated. The physiology of the NADH kinase containing strains was also evaluated during growth on glucose. Overexpressing NADH kinase in the cytosol redirected carbon flow from CO(2) to ethanol during aerobic growth on glucose and to ethanol and acetate during anaerobic growth on glucose. However, cytosolic NADH kinase has an opposite effect during anaerobic metabolism of xylose consumption by channeling carbon flow from ethanol to xylitol. In contrast, overexpressing NADH kinase in the mitochondria did not affect the physiology to a large extent. Overall, although NADH kinase did not increase the rate of xylose consumption, we believe that it can provide an important source of NADPH in yeast, which can be useful for metabolic engineering strategies where the redox fluxes are manipulated.

Space Shuttle Projects

NASA Image and Video Library

1992-05-13

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. This onboard photo depicts Florida’s Atlantic coast and the Cape Canaveral area as the backdrop for this scene of the INTELSAT VI’s approach to the Shuttle Endeavour.

Space Shuttle Projects

NASA Image and Video Library

1992-05-13

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. The 4.5 ton INTELSAT VI was successfully snared by three astronauts on a third EVA. In this photo, the satellite, with its newly deployed perigee stage, begins its separation from the Shuttle.

NAD(H) and NADP(H) Redox Couples and Cellular Energy Metabolism.

PubMed

Xiao, Wusheng; Wang, Rui-Sheng; Handy, Diane E; Loscalzo, Joseph

2018-01-20

The nicotinamide adenine dinucleotide (NAD + )/reduced NAD + (NADH) and NADP + /reduced NADP + (NADPH) redox couples are essential for maintaining cellular redox homeostasis and for modulating numerous biological events, including cellular metabolism. Deficiency or imbalance of these two redox couples has been associated with many pathological disorders. Recent Advances: Newly identified biosynthetic enzymes and newly developed genetically encoded biosensors enable us to understand better how cells maintain compartmentalized NAD(H) and NADP(H) pools. The concept of redox stress (oxidative and reductive stress) reflected by changes in NAD(H)/NADP(H) has increasingly gained attention. The emerging roles of NAD + -consuming proteins in regulating cellular redox and metabolic homeostasis are active research topics. The biosynthesis and distribution of cellular NAD(H) and NADP(H) are highly compartmentalized. It is critical to understand how cells maintain the steady levels of these redox couple pools to ensure their normal functions and simultaneously avoid inducing redox stress. In addition, it is essential to understand how NAD(H)- and NADP(H)-utilizing enzymes interact with other signaling pathways, such as those regulated by hypoxia-inducible factor, to maintain cellular redox homeostasis and energy metabolism. Additional studies are needed to investigate the inter-relationships among compartmentalized NAD(H)/NADP(H) pools and how these two dinucleotide redox couples collaboratively regulate cellular redox states and cellular metabolism under normal and pathological conditions. Furthermore, recent studies suggest the utility of using pharmacological interventions or nutrient-based bioactive NAD + precursors as therapeutic interventions for metabolic diseases. Thus, a better understanding of the cellular functions of NAD(H) and NADP(H) may facilitate efforts to address a host of pathological disorders effectively. Antioxid. Redox Signal. 28, 251-272.

Imaging the NADH:NAD+ Homeostasis for Understanding the Metabolic Response of Mycobacterium to Physiologically Relevant Stresses.

PubMed

Bhat, Shabir A; Iqbal, Iram K; Kumar, Ashwani

2016-01-01

The NADH:NAD + ratio is the primary indicator of the metabolic state of bacteria. NAD(H) homeostasis is critical for Mycobacterium tuberculosis (Mtb) survival and is thus considered an important drug target, but the spatio-temporal measurements of NAD(H) remain a challenge. Genetically encoded fluorescent biosensors of the NADH:NAD + ratios were recently described, paving the way for investigations of the metabolic state of pathogens during infection. Here we have adapted the genetically encoded biosensor Peredox for measurement of the metabolic state of Mtb in vitro and during infection of macrophage cells. Using Peredox, here we show that inhibition of the electron transport chain, disruption of the membrane potential and proton gradient, exposure to reactive oxygen species and treatment with antimycobacterial drugs led to the accumulation of NADH in mycobacterial cells. We have further demonstrated that Mtb residing in macrophages displays higher NADH:NAD + ratios, that may indicate a metabolic stress faced by the intracellular Mtb. We also demonstrate that the Mtb residing in macrophages display a metabolic heterogeneity, which may perhaps explain the tolerance displayed by intracellular Mtb. Next we studied the effect of immunological modulation by interferon gamma on metabolism of intracellular Mtb, since macrophage activation is known to restrict mycobacterial growth. We observed that activation of resting macrophages with interferon-gamma results in higher NADH:NAD + levels in resident Mtb cells. We have further demonstrated that exposure of Isoniazid, Bedaquiline, Rifampicin, and O-floxacin results in higher NADH:NAD + ratios in the Mtb residing in macrophages. However, intracellular Mtb displays lower NADH:NAD + ratio upon exposure to clofazimine. In summary, we have generated reporter strains capable of measuring the metabolic state of Mtb cells in vitro and in vivo with spatio-temporal resolution. We believe that this tool will facilitate further

Influence of oxygen on NADH recycling and oxidative stress resistance systems in Lactobacillus panis PM1

PubMed Central

2013-01-01

Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L. panis PM1 into the stationary phase along with marked changes in end-product profiles. A ten-fold higher concentration of hydrogen peroxide was accumulated during aerobic culture compared to microaerobic culture. This H2O2 level was sufficient for the complete inhibition of L. panis PM1 cell growth, along with a significant reduction in end-products typically found during anaerobic growth. In silico analysis revealed that L. panis possessed two genes for NADH oxidase and NADH peroxidase, but their expression levels were not significantly affected by the presence of oxygen. Specific activities for these two enzymes were observed in crude extracts from L. panis PM1. Enzyme assays demonstrated that the majority of the H2O2 in the culture media was the product of NADH: H2O2 oxidase which was constitutively-active under both aerobic and microaerobic conditions; whereas, NADH peroxidase was positively-activated by the presence of oxygen and had a long induction time in contrast to NADH oxidase. These observations indicated that a coupled NADH oxidase - NADH peroxidase system was the main oxidative stress resistance mechanism in L. panis PM1, and was regulated by oxygen availability. Under aerobic conditions, NADH is mainly reoxidized by the NADH oxidase - peroxidase system rather than through the production of ethanol (or 1,3-propanediol or succinic acid production if glycerol or citric acid is available). This system helped L. panis PM1 directly use oxygen in its energy metabolism by producing extra ATP in contrast to homofermentative lactobacilli. PMID:23369580

Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio.

PubMed

Titov, Denis V; Cracan, Valentin; Goodman, Russell P; Peng, Jun; Grabarek, Zenon; Mootha, Vamsi K

2016-04-08

A decline in electron transport chain (ETC) activity is associated with many human diseases. Although diminished mitochondrial adenosine triphosphate production is recognized as a source of pathology, the contribution of the associated reduction in the ratio of the amount of oxidized nicotinamide adenine dinucleotide (NAD(+)) to that of its reduced form (NADH) is less clear. We used a water-forming NADH oxidase from Lactobacillus brevis (LbNOX) as a genetic tool for inducing a compartment-specific increase of the NAD(+)/NADH ratio in human cells. We used LbNOX to demonstrate the dependence of key metabolic fluxes, gluconeogenesis, and signaling on the cytosolic or mitochondrial NAD(+)/NADH ratios. Expression of LbNOX in the cytosol or mitochondria ameliorated proliferative and metabolic defects caused by an impaired ETC. The results underscore the role of reductive stress in mitochondrial pathogenesis and demonstrate the utility of targeted LbNOX for direct, compartment-specific manipulation of redox state. Copyright © 2016, American Association for the Advancement of Science.

Changes in Oxidative Damage, Inflammation and [NAD(H)] with Age in Cerebrospinal Fluid

PubMed Central

Guest, Jade; Grant, Ross; Mori, Trevor A.; Croft, Kevin D.

2014-01-01

An extensive body of evidence indicates that oxidative stress and inflammation play a central role in the degenerative changes of systemic tissues in aging. However a comparatively limited amount of data is available to verify whether these processes also contribute to normal aging within the brain. High levels of oxidative damage results in key cellular changes including a reduction in available nicotinamide adenine dinucleotide (NAD+), an essential molecule required for a number of vital cellular processes including DNA repair, immune signaling and epigenetic processing. In this study we quantified changes in [NAD(H)] and markers of inflammation and oxidative damage (F2-isoprostanes, 8-OHdG, total antioxidant capacity) in the cerebrospinal fluid (CSF) of healthy humans across a wide age range (24–91 years). CSF was collected from consenting patients who required a spinal tap for the administration of anesthetic. CSF of participants aged >45 years was found to contain increased levels of lipid peroxidation (F2-isoprostanes) (p = 0.04) and inflammation (IL-6) (p = 0.00) and decreased levels of both total antioxidant capacity (p = 0.00) and NAD(H) (p = 0.05), compared to their younger counterparts. A positive association was also observed between plasma [NAD(H)] and CSF NAD(H) levels (p = 0.03). Further analysis of the data identified a relationship between alcohol intake and CSF [NAD(H)] and markers of inflammation. The CSF of participants who consumed >1 standard drink of alcohol per day contained lower levels of NAD(H) compared to those who consumed no alcohol (p<0.05). An increase in CSF IL-6 was observed in participants who reported drinking >0–1 (p<0.05) and >1 (p<0.05) standard alcoholic drinks per day compared to those who did not drink alcohol. Taken together these data suggest a progressive age associated increase in oxidative damage, inflammation and reduced [NAD(H)] in the brain which may be exacerbated by alcohol intake. PMID

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

STS129-S-056 (16 Nov. 2009) --- Members of the space shuttle launch team watch Space Shuttle Atlantis' launch through the newly installed windows of Firing Room 4 in the Launch Control Center at NASA's Kennedy Space Center in Florida. Liftoff of Atlantis from Launch Pad 39A on its STS-129 mission to the International Space Station came at 2:28 p.m. (EST) Nov. 16, 2009.

Spatial dynamics of SIRT1 and the subnuclear distribution of NADH species

PubMed Central

Aguilar-Arnal, Lorena; Ranjit, Suman; Stringari, Chiara; Orozco-Solis, Ricardo; Gratton, Enrico; Sassone-Corsi, Paolo

2016-01-01

Sirtuin 1 (SIRT1) is an NAD+-dependent deacetylase that functions as metabolic sensor of cellular energy and modulates biochemical pathways in the adaptation to changes in the environment. SIRT1 substrates include histones and proteins related to enhancement of mitochondrial function as well as antioxidant protection. Fluctuations in intracellular NAD+ levels regulate SIRT1 activity, but how SIRT1 enzymatic activity impacts on NAD+ levels and its intracellular distribution remains unclear. Here, we show that SIRT1 determines the nuclear organization of protein-bound NADH. Using multiphoton microscopy in live cells, we show that free and bound NADH are compartmentalized inside of the nucleus, and its subnuclear distribution depends on SIRT1. Importantly, SIRT6, a chromatin-bound deacetylase of the same class, does not influence NADH nuclear localization. In addition, using fluorescence fluctuation spectroscopy in single living cells, we reveal that NAD+ metabolism in the nucleus is linked to subnuclear dynamics of active SIRT1. These results reveal a connection between NAD+ metabolism, NADH distribution, and SIRT1 activity in the nucleus of live cells and pave the way to decipher links between nuclear organization and metabolism. PMID:27791113

Space Shuttle Projects

NASA Image and Video Library

2002-03-01

The STS-109 crew of seven waved to onlookers as they emerged from the Operations and Checkout Buildings at Kennedy Space Flight Center eager to get to the launch pad to embark upon the Space Shuttle Orbiter Columbia's 27th flight into space. Crew members included, from front to back, Duane G. Carey (left) and Scott D. Altman (right); Nancy J. Currie, mission specialist; John M. Grunsfield (left), payload commander, and Richard M. Linneham (right); James H. Newman (left) and Michael J. Massimino (right), all mission specialists. Launched March 1, 2002, the goal of the mission was the maintenance and upgrade of the Hubble Space Telescope (HST). The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. By using Columbia's robotic arm, the telescope was captured and secured on a work stand in Columbia's payload bay where four members of the crew performed five spacewalks to complete system upgrades to the HST. Lasting 10 days, 22 hours, and 11 minutes, the STS-109 mission was the 108th flight overall in NASA's Space Shuttle Program.

Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD+-Auxotrophic Mutant ▿

PubMed Central

Zhou, Yongjin; Wang, Lei; Yang, Fan; Lin, Xinping; Zhang, Sufang; Zhao, Zongbao K.

2011-01-01

NAD (NAD+) and its reduced form (NADH) are omnipresent cofactors in biological systems. However, it is difficult to determine the extremes of the cellular NAD(H) level in live cells because the NAD+ level is tightly controlled by a biosynthesis regulation mechanism. Here, we developed a strategy to determine the extreme NAD(H) levels in Escherichia coli cells that were genetically engineered to be NAD+ auxotrophic. First, we expressed the ntt4 gene encoding the NAD(H) transporter in the E. coli mutant YJE001, which had a deletion of the nadC gene responsible for NAD+ de novo biosynthesis, and we showed NTT4 conferred on the mutant strain better growth in the presence of exogenous NAD+. We then constructed the NAD+-auxotrophic mutant YJE003 by disrupting the essential gene nadE, which is responsible for the last step of NAD+ biosynthesis in cells harboring the ntt4 gene. The minimal NAD+ level was determined in M9 medium in proliferating YJE003 cells that were preloaded with NAD+, while the maximal NAD(H) level was determined by exposing the cells to high concentrations of exogenous NAD(H). Compared with supplementation of NADH, cells grew faster and had a higher intracellular NAD(H) level when NAD+ was fed. The intracellular NAD(H) level increased with the increase of exogenous NAD+ concentration, until it reached a plateau. Thus, a minimal NAD(H) level of 0.039 mM and a maximum of 8.49 mM were determined, which were 0.044× and 9.6× those of wild-type cells, respectively. Finally, the potential application of this strategy in biotechnology is briefly discussed. PMID:21742902

STS-118 Space Shuttle Crew Honored

NASA Image and Video Library

2007-09-10

At Walt Disney World in Orlando, the crew members of space shuttle mission STS-118 answer questions from the student audience during a special event to honor the Endeavour crew. Seated from left are Mission Specialists Alvin Drew, Barbara R. Morgan, Dave Williams, Rick Mastracchio and Tracy Caldwell; Pilot Charlie Hobaugh; and Commander Scott Kelly. The event also honored teacher-turned-astronaut Morgan, who dedicated a plaque outside the Mission: Space attraction. Other activities included meeting with the media and a parade down Main Street. Mission STS-118 was the 119th shuttle program flight and the 22nd flight to the International Space Station. Space shuttle Endeavour launched from NASA's Kennedy Space Center on Aug. 8 and landed Aug. 21. The mission delivered the S5 truss, continuing the assembly of the space station.

STS-118 Space Shuttle Crew Honored

NASA Image and Video Library

2007-09-10

Members of the space shuttle mission STS-118 crew march down Main Street at Walt Disney World in Orlando. From left are Mission Specialists Alvin Drew, Barbara R. Morgan and Dave Williams, Pilot Charlie Hobaugh, Mission Specialist Tracy Caldwell and Commander Scott Kelly. Not pictured but present is Mission Specialist Rick Mastracchio. The event also honored teacher-turned-astronaut Morgan, who dedicated a plaque outside the Mission: Space attraction. Other activities included meeting with the media and students. Mission STS-118 was the 119th shuttle program flight and the 22nd flight to the International Space Station. Space shuttle Endeavour launched from NASA's Kennedy Space Center on Aug. 8 and landed Aug. 21. The mission delivered the S5 truss, continuing the assembly of the space station.

Space Shuttle Projects

NASA Image and Video Library

1998-06-08

The STS-95 patch, designed by the crew, is intended to reflect the scientific, engineering, and historic elements of the mission. The Space Shuttle Discovery is shown rising over the sunlit Earth limb, representing the global benefits of the mission science and the solar science objectives of the Spartan Satellite. The bold number '7' signifies the seven members of Discovery's crew and also represents a historical link to the original seven Mercury astronauts. The STS-95 crew member John Glenn's first orbital flight is represented by the Friendship 7 capsule. The rocket plumes symbolize the three major fields of science represented by the mission payloads: microgravity material science, medical research for humans on Earth and in space, and astronomy.

Imaging the NADH:NAD+ Homeostasis for Understanding the Metabolic Response of Mycobacterium to Physiologically Relevant Stresses

PubMed Central

Bhat, Shabir A.; Iqbal, Iram K.; Kumar, Ashwani

2016-01-01

The NADH:NAD+ ratio is the primary indicator of the metabolic state of bacteria. NAD(H) homeostasis is critical for Mycobacterium tuberculosis (Mtb) survival and is thus considered an important drug target, but the spatio-temporal measurements of NAD(H) remain a challenge. Genetically encoded fluorescent biosensors of the NADH:NAD+ ratios were recently described, paving the way for investigations of the metabolic state of pathogens during infection. Here we have adapted the genetically encoded biosensor Peredox for measurement of the metabolic state of Mtb in vitro and during infection of macrophage cells. Using Peredox, here we show that inhibition of the electron transport chain, disruption of the membrane potential and proton gradient, exposure to reactive oxygen species and treatment with antimycobacterial drugs led to the accumulation of NADH in mycobacterial cells. We have further demonstrated that Mtb residing in macrophages displays higher NADH:NAD+ ratios, that may indicate a metabolic stress faced by the intracellular Mtb. We also demonstrate that the Mtb residing in macrophages display a metabolic heterogeneity, which may perhaps explain the tolerance displayed by intracellular Mtb. Next we studied the effect of immunological modulation by interferon gamma on metabolism of intracellular Mtb, since macrophage activation is known to restrict mycobacterial growth. We observed that activation of resting macrophages with interferon-gamma results in higher NADH:NAD+ levels in resident Mtb cells. We have further demonstrated that exposure of Isoniazid, Bedaquiline, Rifampicin, and O-floxacin results in higher NADH:NAD+ ratios in the Mtb residing in macrophages. However, intracellular Mtb displays lower NADH:NAD+ ratio upon exposure to clofazimine. In summary, we have generated reporter strains capable of measuring the metabolic state of Mtb cells in vitro and in vivo with spatio-temporal resolution. We believe that this tool will facilitate further studies on

Identification of mitochondrial electron transport chain-mediated NADH radical formation by EPR spin-trapping techniques.

PubMed

Matsuzaki, Satoshi; Kotake, Yashige; Humphries, Kenneth M

2011-12-20

The mitochondrial electron transport chain (ETC) is a major source of free radical production. However, due to the highly reactive nature of radical species and their short lifetimes, accurate detection and identification of these molecules in biological systems is challenging. The aim of this investigation was to determine the free radical species produced from the mitochondrial ETC by utilizing EPR spin-trapping techniques and the recently commercialized spin-trap, 5-(2,2-dimethyl-1,3-propoxycyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO). We demonstrate that this spin-trap has the preferential quality of having minimal mitochondrial toxicity at concentrations required for radical detection. In rat heart mitochondria and submitochondrial particles supplied with NADH, the major species detected under physiological pH was a carbon-centered radical adduct, indicated by markedly large hyperfine coupling constant with hydrogen (a(H) > 2.0 mT). In the presence of the ETC inhibitors, the carbon-centered radical formation was increased and exhibited NADH concentration dependency. The same carbon-centered radical could also be produced with the NAD biosynthesis precursor, nicotinamide mononucleotide, in the presence of a catalytic amount of NADH. The results support the conclusion that the observed species is a complex I derived NADH radical. The formation of the NADH radical could be blocked by hydroxyl radical scavengers but not SOD. In vitro experiments confirmed that an NADH-radical is readily formed by hydroxyl radical but not superoxide anion, further implicating hydroxyl radical as an upstream mediator of NADH radical production. These findings demonstrate the identification of a novel mitochondrial radical species with potential physiological significance and highlight the diverse mechanisms and sites of production within the ETC.

Paper-Based Device for Rapid Visualization of NADH Based on Dissolution of Gold Nanoparticles.

PubMed

Liang, Pingping; Yu, Haixiang; Guntupalli, Bhargav; Xiao, Yi

2015-07-15

We describe a paper-based device that enables rapid and sensitive room-temperature detection of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric readout and demonstrate its value for monitoring NAD+-driven enzymatic reactions. Our system is based on NADH-mediated inhibition of gold nanoparticle (AuNPs) dissolution in a Au3+-cetyltrimethylammonium bromide (CTAB) solution. We fabricated a device consisting of a mixed cellulose ester paper featuring a wax-encircled, AuNP-coated film atop a cotton absorbent layer sandwiched between two plastic cover layers. In the absence of NADH, the Au3+-CTAB complex dissolves the AuNP layer completely, generating a white color in the test zone. In the presence of NADH, Au3+ is rapidly reduced to Au+, greatly decreasing the dissolution of AuNPs and yielding a red color that becomes stronger at increasing concentrations of NADH. This device exploits capillary force-assisted vertical diffusion, allowing us to apply a 25 μL sample to a surface-confined test zone to achieve a detection limit of 12.5 μM NADH. We used the enzyme glucose dehydrogenase as a model to demonstrate that our paper-based device can monitor NAD+-driven biochemical processes with and without selective dehydrogenase inhibitors by naked-eye observation within 4 min at room temperature in a small sample volume. We believe that our paper-based device could offer a valuable and low-cost analytical tool for monitoring NAD+-associated enzymatic reactions and screening for dehydrogenase inhibitors in a variety of testing contexts.

Determination of NAD + and NADH level in a Single Cell Under H 2O 2 Stress by Capillary Electrophoresis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xi, Wenjun

2008-01-01

A capillary electrophoresis (CE) method is developed to determine both NAD + and NADH levels in a single cell, based on an enzymatic cycling reaction. The detection limit can reach down to 0.2 amol NAD + and 1 amol NADH on a home-made CE-LIF setup. The method showed good reproducibility and specificity. After an intact cell was injected into the inlet of a capillary and lysed using a Tesla coil, intracellular NAD + and NADH were separated, incubated with the cycling buffer, and quantified by the amount of fluorescent product generated. NADH and NAD + levels of single cells ofmore » three cell lines and primary astrocyte culture were determined using this method. Comparing cellular NAD + and NADH levels with and without exposure to oxidative stress induced by H 2O 2, it was found that H9c2 cells respond to the stress by reducing both cellular NAD + and NADH levels, while astrocytes respond by increasing cellular NADH/NAD + ratio.« less

Behavioral Health and Performance Operations During the Space Shuttle Program

NASA Technical Reports Server (NTRS)

Beven, G.; Holland, A.; Moomaw, R.; Sipes, W.; Vander Ark, S.

2011-01-01

Prior to the Columbia STS 107 disaster in 2003, the Johnson Space Center s Behavioral Health and Performance Group (BHP) became involved in Space Shuttle Operations on an as needed basis, occasionally acting as a consultant and primarily addressing crew-crew personality conflicts. The BHP group also assisted with astronaut selection at every selection cycle beginning in 1991. Following STS 107, an event that spawned an increased need of behavioral health support to STS crew members and their dependents, BHP services to the Space Shuttle Program were enhanced beginning with the STS 114 Return to Flight mission in 2005. These services included the presence of BHP personnel at STS launches and landings for contingency support, a BHP briefing to the entire STS crew at L-11 months, a private preflight meeting with the STS Commander at L-9 months, and the presence of a BHP consultant at the L-1.5 month Family Support Office briefing to crew and family members. The later development of an annual behavioral health assessment of all active astronauts also augmented BHP s Space Shuttle Program specific services, allowing for private meetings with all STS crew members before and after each mission. The components of each facet of these BHP Space Shuttle Program support services will be presented, along with valuable lessons learned, and with recommendations for BHP involvement in future short duration space missions

Live cell imaging of cytosolic NADH/NAD+ ratio in hepatocytes and liver slices.

PubMed

Masia, Ricard; McCarty, William J; Lahmann, Carolina; Luther, Jay; Chung, Raymond T; Yarmush, Martin L; Yellen, Gary

2018-01-01

Fatty liver disease (FLD), the most common chronic liver disease in the United States, may be caused by alcohol or the metabolic syndrome. Alcohol is oxidized in the cytosol of hepatocytes by alcohol dehydrogenase (ADH), which generates NADH and increases cytosolic NADH/NAD + ratio. The increased ratio may be important for development of FLD, but our ability to examine this question is hindered by methodological limitations. To address this, we used the genetically encoded fluorescent sensor Peredox to obtain dynamic, real-time measurements of cytosolic NADH/NAD + ratio in living hepatocytes. Peredox was expressed in dissociated rat hepatocytes and HepG2 cells by transfection, and in mouse liver slices by tail-vein injection of adeno-associated virus (AAV)-encoded sensor. Under control conditions, hepatocytes and liver slices exhibit a relatively low (oxidized) cytosolic NADH/NAD + ratio as reported by Peredox. The ratio responds rapidly and reversibly to substrates of lactate dehydrogenase (LDH) and sorbitol dehydrogenase (SDH). Ethanol causes a robust dose-dependent increase in cytosolic NADH/NAD + ratio, and this increase is mitigated by the presence of NAD + -generating substrates of LDH or SDH. In contrast to hepatocytes and slices, HepG2 cells exhibit a relatively high (reduced) ratio and show minimal responses to substrates of ADH and SDH. In slices, we show that comparable results are obtained with epifluorescence imaging and two-photon fluorescence lifetime imaging (2p-FLIM). Live cell imaging with Peredox is a promising new approach to investigate cytosolic NADH/NAD + ratio in hepatocytes. Imaging in liver slices is particularly attractive because it allows preservation of liver microanatomy and metabolic zonation of hepatocytes. NEW & NOTEWORTHY We describe and validate a new approach for measuring free cytosolic NADH/NAD + ratio in hepatocytes and liver slices: live cell imaging with the fluorescent biosensor Peredox. This approach yields dynamic, real

Alterations in cerebral metabolism observed in living rodents using fluorescence lifetime microscopy of intrinsic NADH (Conference Presentation)

NASA Astrophysics Data System (ADS)

Yaseen, Mohammad A.; Sakadžić, Sava; Sutin, Jason; Wu, Weicheng; Fu, Buyin; Boas, David A.

2017-02-01

Monitoring cerebral energy metabolism at a cellular level is essential to improve our understanding of healthy brain function and its pathological alterations. In this study, we resolve specific alterations in cerebral metabolism utilizing minimally-invasive 2-Photon fluorescence lifetime imaging (2P-FLIM) measurements of reduced nicotinamide adenine dinucleotide (NADH) fluorescence, collected in vivo from anesthetized rats and mice. Time-resolved lifetime measurements enables distinction of different components contributing to NADH autofluorescence. These components reportedly represent different enzyme-bound formulations of NADH. Our observations from this study confirm the hypothesis that NADH FLIM can identify specific alterations in cerebral metabolism. Using time-correlated single photon counting (TCSPC) equipment and a custom-built multimodal imaging system, 2-photon fluorescence lifetime imaging (FLIM) was performed in cerebral tissue with high spatial and temporal resolution. Multi-exponential fits for NADH fluorescence lifetimes indicate 4 distinct components, or 'species.' We observed distinct variations in the relative proportions of these components before and after pharmacological-induced impairments to several reactions involved in anaerobic glycolysis and aerobic oxidative metabolism. Classification models developed with experimental data correctly predict the metabolic impairments associated with bicuculline-induced focal seizures in separate experiments. Compared to traditional intensity-based NADH measurements, lifetime imaging of NADH is less susceptible to the adverse effects of overlying blood vessels. Evaluating NADH measurements will ultimately lead to a deeper understanding of cerebral energetics and its pathology-related alterations. Such knowledge will likely aid development of therapeutic strategies for neurodegenerative diseases such as Alzheimer's Disease, Parkinson's disease, and stroke.

Space Shuttle Projects

NASA Image and Video Library

1991-08-02

Launched aboard the Space Shuttle Atlantis on August 2, 1991, the STS-43 mission’s primary payload was the Tracking and Data Relay Satellite 5 (TDRS-5) attached to an Inertial Upper Stage (IUS), which became the 4th member of an orbiting TDRS cluster. The flight crew consisted of 5 astronauts: John E. Blaha, commander; Michael A. Baker, pilot; Shannon W. Lucid, mission specialist 1; James C. Adamson, mission specialist 2; and G. David Low, mission specialist 3.

Space Shuttle Projects

NASA Image and Video Library

1991-08-02

Launched aboard the Space Shuttle Atlantis on August 2, 1991, the STS-43 mission’s primary payload was the Tracking and Data Relay Satellite 5 (TDRS-5) attached to an Inertial Upper Stage (IUS), which became the 4th member of an orbiting TDRS cluster. The flight crew consisted of five astronauts: John E. Blaha, commander; Michael A. Baker, pilot; Shannon W. Lucid, mission specialist 1; James C. Adamson, mission specialist 2; and G. David Low, mission specialist 3.

STS-118 Space Shuttle Crew Honored

NASA Image and Video Library

2007-09-10

A reporter interviews STS-118 Mission Specialist Dave Williams during a special event at Walt Disney World in Orlando . The day's events honoring the STS-118 space shuttle crew recognized the inspirational achievement of teacher-turned-astronaut Barbara R. Morgan who helped dedicate a plaque outside the Mission: Space attraction, and included meeting with students and the media and parading down Main Street to the delight of the crowds. The other crew members attending were Commander Scott Kelly, Pilot Charlie Hobaugh and Mission Specialists Tracy Caldwell, Rick Mastracchio and Alvin Drew. Mission STS-118 was the 119th shuttle program flight and the 22nd flight to the International Space Station. Space shuttle Endeavour launched from NASA's Kennedy Space Center on Aug. 8 and landed Aug. 21. The mission delivered the S5 truss, continuing the assembly of the space station

Dissection of the Caffeate Respiratory Chain in the Acetogen Acetobacterium woodii: Identification of an Rnf-Type NADH Dehydrogenase as a Potential Coupling Site▿

PubMed Central

Imkamp, Frank; Biegel, Eva; Jayamani, Elamparithi; Buckel, Wolfgang; Müller, Volker

2007-01-01

The anaerobic acetogenic bacterium Acetobacterium woodii couples caffeate reduction with electrons derived from hydrogen to the synthesis of ATP by a chemiosmotic mechanism with sodium ions as coupling ions, a process referred to as caffeate respiration. We addressed the nature of the hitherto unknown enzymatic activities involved in this process and their cellular localization. Cell extract of A. woodii catalyzes H2-dependent caffeate reduction. This reaction is strictly ATP dependent but can be activated also by acetyl coenzyme A (CoA), indicating that there is formation of caffeyl-CoA prior to reduction. Two-dimensional gel electrophoresis revealed proteins present only in caffeate-grown cells. Two proteins were identified by electrospray ionization-mass spectrometry/mass spectrometry, and the encoding genes were cloned. These proteins are very similar to subunits α (EtfA) and β (EtfB) of electron transfer flavoproteins present in various anaerobic bacteria. Western blot analysis demonstrated that they are induced by caffeate and localized in the cytoplasm. Etf proteins are known electron carriers that shuttle electrons from NADH to different acceptors. Indeed, NADH was used as an electron donor for cytosolic caffeate reduction. Since the hydrogenase was soluble and used ferredoxin as an electron acceptor, the missing link was a ferredoxin:NAD+ oxidoreductase. This activity could be determined and, interestingly, was membrane bound. A search for genes that could encode this activity revealed DNA fragments encoding subunits C and D of a membrane-bound Rnf-type NADH dehydrogenase that is a potential Na+ pump. These data suggest the following electron transport chain: H2 → ferredoxin → NAD+ → Etf → caffeyl-CoA reductase. They also imply that the sodium motive step in the chain is the ferredoxin-dependent NAD+ reduction catalyzed by Rnf. PMID:17873051

Space Shuttle Projects

NASA Image and Video Library

1993-03-30

Designed by the mission’s crew members, the STS-57 crew patch depicts the Space Shuttle Endeavour maneuvering to retrieve the European Space Agency's microgravity experiment satellite EURECA. SpaceHab, the first commercial space laboratory, is depicted in the cargo bay, and its characteristic shape is represented by the inner red border of the patch. The three gold plumes surrounded by the five stars trailing EURECA are suggestive of the U.S. astronaut logo. The five gold stars together with the shape of the orbiter's mechanical arm form the mission's numerical designation. The six stars on the American flag represent the U.S. astronauts who comprise the crew. With detailed input from the crew members, the final artwork was accomplished by artist Tim Hall.

Detection of ATP and NADH: A Bioluminescent Experience.

ERIC Educational Resources Information Center

Selig, Ted C.; And Others

1984-01-01

Described is a bioluminescent assay for adenosine triphosphate (ATP) and reduced nicotineamide-adenine dinucleotide (NADH) that meets the requirements of an undergraduate biochemistry laboratory course. The 3-hour experiment provides students with experience in bioluminescence and analytical biochemistry yet requires limited instrumentation,…

Launch of space shuttle Challenger on the 41-C mission

NASA Image and Video Library

1984-04-06

41C-3029 (6 April 1984) --- The space shuttle Challenger and its five-member astronaut crew leave the launch pad at the Kennedy Space Center to begin a six-day stay in space. Astronaut John W. Young, a veteran of two shuttle missions and six spaceflights overall, recorded the image with a handheld 70mm camera from the shuttle training aircraft which he was using to monitor environmental conditions around Florida. This is the eighth mission on which Young photographed one of NASA's orbiter vehicles beginning its orbital stay. Photo credit: NASA

Space Shuttle Projects

NASA Image and Video Library

2002-03-03

The Hubble Space Telescope (HST), with its normal routine temporarily interrupted, is about to be captured by the Space Shuttle Columbia prior to a week of servicing and upgrading by the STS-109 crew. The telescope was captured by the shuttle's Remote Manipulator System (RMS) robotic arm and secured on a work stand in Columbia's payload bay where 4 of the 7-member crew performed 5 space walks completing system upgrades to the HST. Included in those upgrades were: The replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when its original coolant ran out. The Marshall Space Flight Center had the responsibility for the design, development, and construction of the the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. Launched March 1, 2002, the STS-109 HST servicing mission lasted 10 days, 22 hours, and 11 minutes. It was the 108th flight overall in NASA's Space Shuttle Program.

Aluminum and its effect in the equilibrium between folded/unfolded conformation of NADH.

PubMed

Formoso, Elena; Mujika, Jon I; Grabowski, Slawomir J; Lopez, Xabier

2015-11-01

Nicotinamide adenine dinucleotide (NADH) is one of the most abundant cofactor employed by proteins and enzymes. The molecule is formed by two nucleotides that can lead to two main conformations: folded/closed and unfolded/open. Experimentally, it has been determined that the closed form is about 2 kcal/mol more stable than the open formed. Computationally, a correct description of the NADH unfolding process is challenging due to different reasons: 1) The unfolding process shows a very low energy difference between the two conformations 2) The molecule can form a high number of internal hydrogen bond interactions 3) Subtle effects such as dispersion may be important. In order to tackle all these effects, we have employed a number of different state of the art computational techniques, including: a) well-tempered metadynamics, b) geometry optimizations, and c) Quantum Theory of Atoms in Molecules (QTAIM) calculations, to investigate the conformational change of NADH in solution and interacting with aluminum. All the results indicate that aluminum indeed favors the closed conformation of NADH, due mainly to the formation of a more rigid structure through key hydrogen bond interactions. Copyright © 2015 Elsevier Inc. All rights reserved.

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuzu, Mutlu; Niefind, Karsten; Hummel, Werner

2005-05-01

The water-forming flavoenzyme NADH oxidase was crystallized successfully for the first time. The crystals diffract X-rays to at least 4.0 Å resolution. NADH oxidase (NOX) from Lactobacillus brevis is a homotetrameric flavoenzyme composed of 450 amino acids per subunit. The molecular weight of each monomer is 48.8 kDa. The enzyme catalyzes the oxidation of two equivalents of NADH and reduces one equivalent of oxygen to yield two equivalents of water, without releasing hydrogen peroxide after the reduction of the first equivalent of NADH. Crystals of this protein were grown in the presence of 34% polyethylene glycol monomethyl ether 2000, 0.1more » M sodium acetate and 0.2 M ammonium sulfate at pH 5.4. They belong to the tetragonal space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = 74.8, b = 95.7, c = 116.9 Å, α = γ = 90, β = 103.8°. The current diffraction limit is 4.0 Å. The self-rotation function of the native data set is consistent with a NOX tetramer in the asymmetric unit.« less

Cloning and mRNA Expression of NADH Dehydrogenase during Ochlerotatus taeniorhynchus Development and Pesticide Response

USDA-ARS?s Scientific Manuscript database

NADH dehydrogenase, the largest of the respiratory complexes, is the first enzyme of the mitochondrial electron transport chain. We have cloned and sequenced cDNA of NADH dehydrogenase gene from Ochlerotatus (Ochlerotatus) taeniorhynchus (Wiedemann) adult (GeneBank Accession number: FJ458415). The ...

Single sample extraction and HPLC processing for quantification of NAD and NADH levels in Saccharomyces cerevisiae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sporty, J; Kabir, M M; Turteltaub, K

A robust redox extraction protocol for quantitative and reproducible metabolite isolation and recovery has been developed for simultaneous measurement of nicotinamide adenine dinucleotide (NAD) and its reduced form, NADH, from Saccharomyces cerevisiae. Following culture in liquid media, approximately 10{sup 8} yeast cells were harvested by centrifugation and then lysed under non-oxidizing conditions by bead blasting in ice-cold, nitrogen-saturated 50-mM ammonium acetate. To enable protein denaturation, ice cold nitrogen-saturated CH{sub 3}CN + 50-mM ammonium acetate (3:1; v:v) was added to the cell lysates. After sample centrifugation to pellet precipitated proteins, organic solvent removal was performed on supernatants by chloroform extraction. Themore » remaining aqueous phase was dried and resuspended in 50-mM ammonium acetate. NAD and NADH were separated by HPLC and quantified using UV-VIS absorbance detection. Applicability of this procedure for quantifying NAD and NADH levels was evaluated by culturing yeast under normal (2% glucose) and calorie restricted (0.5% glucose) conditions. NAD and NADH contents are similar to previously reported levels in yeast obtained using enzymatic assays performed separately on acid (for NAD) and alkali (for NADH) extracts. Results demonstrate that it is possible to perform a single preparation to reliably and robustly quantitate both NAD and NADH contents in the same sample. Robustness of the protocol suggests it will be (1) applicable to quantification of these metabolites in mammalian and bacterial cell cultures; and (2) amenable to isotope labeling strategies to determine the relative contribution of specific metabolic pathways to total NAD and NADH levels in cell cultures.« less

STS-69 Crew members display 'Dog Crew' patches

NASA Technical Reports Server (NTRS)

1995-01-01

Following their arrival at KSC's Shuttle Landing Facility, the five astronauts assigned to Space Shuttle Mission STS-69 display the unofficial crew patch for their upcoming spaceflight: the Dog Crew II patch. Mission Commander David M. Walker (center) and Payload Commander James S. Voss (second from right) previously flew together on Mission STS-53, the final dedicated Department of Defense flight on the Space Shuttle. A close comradery formed among Walker, Voss and the rest of the crew, and they dubbed themselves the 'dogs of war', with each of the STS-53 'Dog Crew' members assigned a 'dog tag' or nickname. When the STS-69 astronauts also became good buddies, they decided it was time for the Dog Crew II to be named. Walker's dog tag is Red Dog, Voss's is Dogface, Pilot Kenneth D. Cockrell (second from left) is Cujo, space rookie and Mission Specialist Michael L. Gernhardt (left) is Under Dog, and Mission Specialist James H. Newman (right) is Pluato. The Dog Crew II patch features a bulldog peering out from a doghouse shaped like the Space Shuttle and lists the five crew member's dog names. The five astronauts are scheduled to lift off on the fifth Shuttle flight of the year at 11:04 a.m. EDT, August 31, aboard the Space Shuttle Endeavour.

Extremely high intracellular concentration of glucose-6-phosphate and NAD(H) in Deinococcus radiodurans.

PubMed

Yamashiro, Takumi; Murata, Kousaku; Kawai, Shigeyuki

2017-03-01

Deinococcus radiodurans is highly resistant to ionizing radiation and UV radiation, and oxidative stress caused by such radiations. NADP(H) seems to be important for this resistance (Slade and Radman, Microbiol Mol Biol Rev 75:133-191; Slade, Radman, Microbiol Mol Biol Rev 75:133-191, 2011), but the mechanism underlying the generation of NADP(H) or NAD(H) in D. radiodurans has not fully been addressed. Intracellular concentrations of NAD + , NADH, NADP + , and NADPH in D. radiodurans are also not determined yet. We found that cell extracts of D. radiodurans catalyzed reduction of NAD(P) + in vitro, indicating that D. radiodurans cells contain both enzymes and a high concentration of substrates for this activity. The enzyme and the substrate were attributed to glucose-6-phosphate dehydrogenase and glucose-6-phosphate of which intracellular concentration was extremely high. Unexpectedly, the intracellular concentration of NAD(H) was also much greater than that of NADP(H), suggesting some significant roles of NADH. These unusual features of this bacterium would shed light on a new aspect of physiology of this bacterium.

STS-118 Space Shuttle Crew Honored

NASA Image and Video Library

2007-09-10

NASA's Kennedy Space Center Education Specialists Linda Scauzillo and Christopher Blair take part in a special education session with local students at Epcot's Base21 Siemens VIP Center. The event was part of the day's activities honoring the space shuttle Endeavour crew of mission STS-118. The crew met with the media and paraded down Main Street. The event also honored teacher-turned-astronaut Barbara R. Morgan, who dedicated a plaque outside the Mission: Space attraction. The other crew members attending were Commander Scott Kelly, Pilot Charlie Hobaugh and Mission Specialists Tracy Caldwell, Dave Williams, Rick Mastracchio and Alvin Drew. Mission STS-118 was the 119th shuttle program flight and the 22nd flight to the International Space Station. Space shuttle Endeavour launched from NASA's Kennedy Space Center on Aug. 8 and landed Aug. 21. The mission delivered the S5 truss, continuing the assembly of the space station.

Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass.

PubMed

Han, Lei; Liang, Bo; Song, Jianxia

2018-02-01

As an important cofactor, NADH is essential for most redox reactions and biofuel cells. However, supply of exogenous NADH is challenged, due to the low production efficiency and high cost of NADH regeneration system, as well as low stability of NADH. Here, we constructed a novel cell surface multi-enzyme co-display system with ratio- and space-controllable manner as exogenous NADH regeneration system for the sustainable NADH production from low-cost biomass. Dockerin-fused glucoamylase (GA) and glucose dehydrogenase (GDH) were expressed and assembled on the engineered bacterial surfaces, which displayed protein scaffolds with various combinations of different cohesins. When the ratio of GA and GDH was 3:1, the NADH production rate of the whole-cell biocatalyst reached the highest level using starch as substrate, which was three times higher than that of mixture of free enzymes, indicating that the highly ordered spatial organization of enzymes would promote reactions, due to the ratio of enzymes and proximity effect. To confirm performance of the established NADH regeneration system, the highly efficient synthesis of L-lactic acid (L-LA) was conducted by the system and the yield of L-LA (16 g/L) was twice higher than that of the mixture of free enzymes. The multi-enzyme co-display system showed good stability in the cyclic utilization. In conclusion, the novel sustainable NADH system would provide a cost-effective strategy to regenerate cofactor from low-cost biomass.

STS-125 Crew Members replace LiOH Cannisters on the Shuttle Atlantis Middeck

NASA Image and Video Library

2009-05-13

S125-E-006611 (13 May 2009) --- Astronaut John Grunsfeld, STS-125 mission specialist, works with lithium hydroxide (LiOH) canisters from beneath Space Shuttle Atlantis' middeck during flight day three activities.

Phasor Fluorescence Lifetime Microscopy of Free and Protein-Bound NADH Reveals Neural Stem Cell Differentiation Potential

PubMed Central

Stringari, Chiara; Nourse, Jamison L.; Flanagan, Lisa A.; Gratton, Enrico

2012-01-01

In the stem cell field there is a lack of non invasive and fast methods to identify stem cell’s metabolic state, differentiation state and cell-lineage commitment. Here we describe a label-free method that uses NADH as an intrinsic biomarker and the Phasor approach to Fluorescence Lifetime microscopy to measure the metabolic fingerprint of cells. We show that different metabolic states are related to different cell differentiation stages and to stem cell bias to neuronal and glial fate, prior the expression of lineage markers. Our data demonstrate that the NADH FLIM signature distinguishes non-invasively neurons from undifferentiated neural progenitor and stem cells (NPSCs) at two different developmental stages (E12 and E16). NPSCs follow a metabolic trajectory from a glycolytic phenotype to an oxidative phosphorylation phenotype through different stages of differentiation. NSPCs are characterized by high free/bound NADH ratio, while differentiated neurons are characterized by low free/bound NADH ratio. We demonstrate that the metabolic signature of NPSCs correlates with their differentiation potential, showing that neuronal progenitors and glial progenitors have a different free/bound NADH ratio. Reducing conditions in NPSCs correlates with their neurogenic potential, while oxidative conditions correlate with glial potential. For the first time we show that FLIM NADH metabolic fingerprint provides a novel, and quantitative measure of stem cell potential and a label-free and non-invasive means to identify neuron- or glial- biased progenitors. PMID:23144844

Space Shuttle Projects

NASA Image and Video Library

1995-06-02

These five NASA astronauts were the crew members for the STS-69 mission that launched aboard the Space Shuttle Endeavour September 7, 1995. Pictured on the front row (left to right) are David M. Walker, mission commander; and Kenneth D. Cockrell, pilot. On the back row (left to right) are Michael L. Gernhardt and James H. Newman, both mission specialists; and James S. Voss, payload commander. The mission’s two primary payloads included the Spartan 201-3 and Wake Shield Facility-2 (WSF-2).

Space Shuttle Projects

NASA Image and Video Library

1990-07-08

The STS-40 patch makes a contemporary statement focusing on human beings living and working in space. Against a background of the universe, seven silver stars, interspersed about the orbital path of Columbia, represent the seven crew members. The orbiter's flight path forms a double-helix, designed to represent the DNA molecule common to all living creatures. In the words of a crew spokesman, ...(the helix) affirms the ceaseless expansion of human life and American involvement in space while simultaneously emphasizing the medical and biological studies to which this flight is dedicated. Above Columbia, the phrase Spacelab Life Sciences 1 defines both the Shuttle mission and its payload. Leonardo Da Vinci's Vitruvian man, silhouetted against the blue darkness of the heavens, is in the upper center portion of the patch. With one foot on Earth and arms extended to touch Shuttle's orbit, the crew feels, he serves as a powerful embodiment of the extension of human inquiry from the boundaries of Earth to the limitless laboratory of space. Sturdily poised amid the stars, he serves to link scentists on Earth to the scientists in space asserting the harmony of efforts which produce meaningful scientific spaceflight missions. A brilliant red and yellow Earth limb (center) links Earth to space as it radiates from a native American symbol for the sun. At the frontier of space, the traditional symbol for the sun vividly links America's past to America's future, the crew states. Beneath the orbiting Shuttle, darkness of night rests peacefully over the United States. Drawn by artist Sean Collins, the STS 40 Space Shuttle patch was designed by the crewmembers for the flight.

Investigation of the NADH/NAD+ ratio in Ralstonia eutropha using the fluorescence reporter protein Peredox.

PubMed

Tejwani, Vijay; Schmitt, Franz-Josef; Wilkening, Svea; Zebger, Ingo; Horch, Marius; Lenz, Oliver; Friedrich, Thomas

2017-01-01

Ralstonia eutropha is a hydrogen-oxidizing ("Knallgas") bacterium that can easily switch between heterotrophic and autotrophic metabolism to thrive in aerobic and anaerobic environments. Its versatile metabolism makes R. eutropha an attractive host for biotechnological applications, including H 2 -driven production of biodegradable polymers and hydrocarbons. H 2 oxidation by R. eutropha takes place in the presence of O 2 and is mediated by four hydrogenases, which represent ideal model systems for both biohydrogen production and H 2 utilization. The so-called soluble hydrogenase (SH) couples reversibly H 2 oxidation with the reduction of NAD + to NADH and has already been applied successfully in vitro and in vivo for cofactor regeneration. Thus, the interaction of the SH with the cellular NADH/NAD + pool is of major interest. In this work, we applied the fluorescent biosensor Peredox to measure the [NADH]:[NAD + ] ratio in R. eutropha cells under different metabolic conditions. The results suggest that the sensor operates close to saturation level, indicating a rather high [NADH]:[NAD + ] ratio in aerobically grown R. eutropha cells. Furthermore, we demonstrate that multicomponent analysis of spectrally-resolved fluorescence lifetime data of the Peredox sensor response to different [NADH]:[NAD + ] ratios represents a novel and sensitive tool to determine the redox state of cells. Copyright © 2016 Elsevier B.V. All rights reserved.

APPROACH & LANDING TEST (ALT) - SHUTTLE PATCH

NASA Image and Video Library

1976-11-01

S76-30340 (1976) --- This circular, red, white and blue emblem has been chosen as the official insignia for the Space Shuttle Approach and Landing Test (ALT) flights. A picture of the Orbiter 101 "Enterprise" is superimposed over a red triangle, which in turn is superimposed over a large inner circle of dark blue. The surnames of the members of the two ALT crews are in white in the field of blue. The four crew men are astronauts Fred W. Haise Jr., commander of the first crew; Joe H. Engle, commander of the second crew; and Richard H. Truly, pilot of the second crew. ALT is a series of flights with a modified Boeing 747 Shuttle Carrier Aircraft (SCA) as a ferry aircraft and airborne launch platform for the 67,300 kilogram (75-ton) "Enterprise". The Shuttle Orbiter atmospheric testing is in preparation for the first Earth-orbital flights scheduled in 1979.

Two-photon NADH imaging exposes boundaries of oxygen diffusion in cortical vascular supply regions

PubMed Central

Kasischke, Karl A; Lambert, Elton M; Panepento, Ben; Sun, Anita; Gelbard, Harris A; Burgess, Robert W; Foster, Thomas H; Nedergaard, Maiken

2011-01-01

Oxygen transport imposes a possible constraint on the brain's ability to sustain variable metabolic demands, but oxygen diffusion in the cerebral cortex has not yet been observed directly. We show that concurrent two-photon fluorescence imaging of endogenous nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation exposes well-defined boundaries of tissue oxygen diffusion in the mouse cortex. The NADH fluorescence increases rapidly over a narrow, very low pO2 range with a p50 of 3.4±0.6 mm Hg, thereby establishing a nearly binary reporter of significant, metabolically limiting hypoxia. The transient cortical tissue boundaries of NADH fluorescence exhibit remarkably delineated geometrical patterns, which define the limits of tissue oxygen diffusion from the cortical microcirculation and bear a striking resemblance to the ideal Krogh tissue cylinder. The visualization of microvessels and their regional contribution to oxygen delivery establishes penetrating arterioles as major oxygen sources in addition to the capillary network and confirms the existence of cortical oxygen fields with steep microregional oxygen gradients. Thus, two-photon NADH imaging can be applied to expose vascular supply regions and to localize functionally relevant microregional cortical hypoxia with micrometer spatial resolution. PMID:20859293

Two-photon NADH imaging exposes boundaries of oxygen diffusion in cortical vascular supply regions.

PubMed

Kasischke, Karl A; Lambert, Elton M; Panepento, Ben; Sun, Anita; Gelbard, Harris A; Burgess, Robert W; Foster, Thomas H; Nedergaard, Maiken

2011-01-01

Oxygen transport imposes a possible constraint on the brain's ability to sustain variable metabolic demands, but oxygen diffusion in the cerebral cortex has not yet been observed directly. We show that concurrent two-photon fluorescence imaging of endogenous nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation exposes well-defined boundaries of tissue oxygen diffusion in the mouse cortex. The NADH fluorescence increases rapidly over a narrow, very low pO(2) range with a p(50) of 3.4 ± 0.6 mm Hg, thereby establishing a nearly binary reporter of significant, metabolically limiting hypoxia. The transient cortical tissue boundaries of NADH fluorescence exhibit remarkably delineated geometrical patterns, which define the limits of tissue oxygen diffusion from the cortical microcirculation and bear a striking resemblance to the ideal Krogh tissue cylinder. The visualization of microvessels and their regional contribution to oxygen delivery establishes penetrating arterioles as major oxygen sources in addition to the capillary network and confirms the existence of cortical oxygen fields with steep microregional oxygen gradients. Thus, two-photon NADH imaging can be applied to expose vascular supply regions and to localize functionally relevant microregional cortical hypoxia with micrometer spatial resolution.

Investigation of the Ionization Mechanism of NAD+/NADH-Modified Gold Electrodes in ToF-SIMS Analysis.

PubMed

Hua, Xin; Zhao, Li-Jun; Long, Yi-Tao

2018-06-04

Analysis of nicotinamide adenine dinucleotide (NAD + /NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD + /NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD + /NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD + and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes. Graphical Abstract ᅟ.

Space Shuttle Project

NASA Image and Video Library

1992-10-22

The Space Shuttle Columbia (STS-52) thunders off Launch Pad 39B, embarking on a 10-day flight and carrying a crew of six who will deploy the Laser Geodynamic Satellite II (LAGEOS). LAGEOS is a spherical passive satellite covered with reflectors which are illuminated by ground-based lasers to determine precise measurements of the Earth's crustal movements. The other major payload on this mission is the United States Microgravity Payload 1 (USMP-1), where experiments will be conducted by crew members while in low earth orbit (LEO).

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH

DOE PAGES

Brinkmann-Chen, Sabine; Flock, Tilman; Cahn, Jackson K. B.; ...

2013-06-17

To date, efforts to switch the cofactor specificity of oxidoreductases from nicotinamide adenine dinucleotide phosphate (NADPH) to nicotinamide adenine dinucleotide (NADH) have been made on a case-by-case basis with varying degrees of success. Here we present a straightforward recipe for altering the cofactor specificity of a class of NADPH-dependent oxidoreductases, the ketol-acid reductoisomerases (KARIs). Combining previous results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, we identified key cofactor specificity determinants and used this information to construct five KARIs with reversed cofactor preference. Additional directed evolution generated two enzymesmore » having NADH-dependent catalytic efficiencies that are greater than the wild-type enzymes with NADPH. As a result, high-resolution structures of a wild-type/variant pair reveal the molecular basis of the cofactor switch.« less

Hubble Space Telescope nears Shuttle Endeavour

NASA Image and Video Library

1993-12-04

STS061-73-040 (4 Dec 1993) --- Backdropped against the blackness of space, the Hubble Space Telescope (HST) nears the Space Shuttle Endeavour. With the aid of the Remote Manipulator System (RMS), the STS-61 crew members later grappled the spacecraft and berthed it in the cargo bay for five-days of servicing chores by four space walkers.

Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin.

PubMed

Zhao, Gang; Zhang, Hui; Chen, Xi; Zhu, Xifang; Guo, Yusi; He, Chenfei; Anwar Khan, Farhan; Chen, Yingyu; Hu, Changmin; Chen, Huanchun; Guo, Aizhen

2017-03-03

Mycoplasma bovis causes considerable economic losses in the cattle industry worldwide. In mycoplasmal infections, adhesion to the host cell is of the utmost importance. In this study, the amino acid sequence of NOX was predicted to have enzymatic domains. The nox gene was then cloned and expressed in Escherichia coli. The enzymatic activity of recombinant NOX (rNOX) was confirmed based on its capacity to oxidize NADH to NAD + and reduce O 2 to H 2 O 2 . The adherence of rNOX to embryonic bovine lung (EBL) cells was confirmed with confocal laser scanning microscopy, enzyme-linked immunosorbent assay, and flow cytometry. Both preblocking EBL cells with purified rNOX and preneutralizing M. bovis with polyclonal antiserum to rNOX significantly reduced the adherence of M. bovis to EBL cells. Mycoplasma bovis NOX- expressed a truncated NOX protein at a level 10-fold less than that of the wild type. The capacities of M. bovis NOX- for cell adhesion and H 2 O 2 production were also significantly reduced. The rNOX was further used to pan phage displaying lung cDNA library and fibronectin was determined to be potential ligand. In conclusion, M. bovis NOX functions as both an active NADH oxidase and adhesin, and is therefore a potential virulence factor.

Crosstalk of Signaling and Metabolism Mediated by the NAD(+)/NADH Redox State in Brain Cells.

PubMed

Winkler, Ulrike; Hirrlinger, Johannes

2015-12-01

The energy metabolism of the brain has to be precisely adjusted to activity to cope with the organ's energy demand, implying that signaling regulates metabolism and metabolic states feedback to signaling. The NAD(+)/NADH redox state constitutes a metabolic node well suited for integration of metabolic and signaling events. It is affected by flux through metabolic pathways within a cell, but also by the metabolic state of neighboring cells, for example by lactate transferred between cells. Furthermore, signaling events both in neurons and astrocytes have been reported to change the NAD(+)/NADH redox state. Vice versa, a number of signaling events like astroglial Ca(2+) signals, neuronal NMDA-receptors as well as the activity of transcription factors are modulated by the NAD(+)/NADH redox state. In this short review, this bidirectional interdependence of signaling and metabolism involving the NAD(+)/NADH redox state as well as its potential relevance for the physiology of the brain and the whole organism in respect to blood glucose regulation and body weight control are discussed.

NADH/NADPH bi-cofactor-utilizing and thermoactive ketol-acid reductoisomerase from Sulfolobus acidocaldarius.

PubMed

Chen, Chin-Yu; Ko, Tzu-Ping; Lin, Kuan-Fu; Lin, Bo-Lin; Huang, Chun-Hsiang; Chiang, Cheng-Hung; Horng, Jia-Cherng

2018-05-08

Ketol-acid reductoisomerase (KARI) is a bifunctional enzyme in the second step of branched-chain amino acids biosynthetic pathway. Most KARIs prefer NADPH as a cofactor. However, KARI with a preference for NADH is desirable in industrial applications including anaerobic fermentation for the production of branched-chain amino acids or biofuels. Here, we characterize a thermoacidophilic archaeal Sac-KARI from Sulfolobus acidocaldarius and present its crystal structure at a 1.75-Å resolution. By comparison with other holo-KARI structures, one sulphate ion is observed in each binding site for the 2'-phosphate of NADPH, implicating its NADPH preference. Sac-KARI has very high affinity for NADPH and NADH, with K M values of 0.4 μM for NADPH and 6.0 μM for NADH, suggesting that both are good cofactors at low concentrations although NADPH is favoured over NADH. Furthermore, Sac-KARI can catalyze 2(S)-acetolactate (2S-AL) with either cofactor from 25 to 60 °C, but the enzyme has higher activity by using NADPH. In addition, the catalytic activity of Sac-KARI increases significantly with elevated temperatures and reaches an optimum at 60 °C. Bi-cofactor utilization and the thermoactivity of Sac-KARI make it a potential candidate for use in metabolic engineering or industrial applications under anaerobic or harsh conditions.

Characterization of Frex as an NADH sensor for in vivo applications in the presence of NAD+ and at various pH values.

PubMed

Wilkening, Svea; Schmitt, Franz-Josef; Horch, Marius; Zebger, Ingo; Lenz, Oliver; Friedrich, Thomas

2017-09-01

The fluorescent biosensor Frex, recently introduced as a sensitive tool to quantify the NADH concentration in living cells, was characterized by time-integrated and time-resolved fluorescence spectroscopy regarding its applicability for in vivo measurements. Based on the purified sensor protein, it is shown that the NADH dependence of Frex fluorescence can be described by a Hill function with a concentration of half-maximal sensor response of K D  ≈ 4 µM and a Hill coefficient of n ≈ 2. Increasing concentrations of NADH have moderate effects on the fluorescence lifetime of Frex, which changes by a factor of two from about 500 ps in the absence of NADH to 1 ns under fluorescence-saturating NADH concentrations. Therefore, the observed sevenfold rise of the fluorescence intensity is primarily ascribed to amplitude changes. Notably, the dynamic range of Frex sensitivity towards NADH highly depends on the NAD + concentration, while the apparent K D for NADH is only slightly affected. We found that NAD + has a strong inhibitory effect on the fluorescence response of Frex during NADH sensing, with an apparent NAD + dissociation constant of K I  ≈ 400 µM. This finding was supported by fluorescence lifetime measurements, which showed that the addition of NAD + hardly affects the fluorescence lifetime, but rather reduces the number of Frex molecules that are able to bind NADH. Furthermore, the fluorescence responses of Frex to NADH and NAD + depend critically on pH and temperature. Thus, for in vivo applications of Frex, temperature and pH need to be strictly controlled or considered during data acquisition and analysis. If all these constraints are properly met, Frex fluorescence intensity measurements can be employed to estimate the minimum NADH concentration present within the cell at sufficiently low NAD + concentrations below 100 µM.

STS-125 Crew Members replace LiOH Cannisters on the Shuttle Atlantis Middeck

NASA Image and Video Library

2009-05-13

S125-E-006610 (13 May 2009) --- Astronauts John Grunsfeld (bottom), STS-125 mission specialist; and Gregory C. Johnson (partially out of frame), pilot, work with lithium hydroxide (LiOH) canisters from beneath Space Shuttle Atlantis' middeck during flight day three activities.

NADH induces the generation of superoxide radicals in leaf peroxisomes. [Pisum sativum L

DOE Office of Scientific and Technical Information (OSTI.GOV)

del Rio, L.A.; Sandalio, L.M.; Palma, J.M.

1989-03-01

In peroxisomes isolated from pea leaves (Pisum sativum L.) the production of superoxide free radicals (O{sub 2}{sup {minus}}) by xanthine and NADH was investigated. In peroxisomal membranes, 100 micromolar NADH induced the production of O{sub 2}{sup {minus}} radicals. In the soluble fractions of peroxisomes, no generation of O{sub 2}{sup {minus}} radicals was observed by incubation with either NADH or xanthine, although xanthine oxidase was found located predominantly in the matrix of peroxisomes. The failure of xanthine to induce superoxide generation was probably due to the inability to fully suppress the endogenous Mn-superoxide dismutase activity by inhibitors which were inactive againstmore » xanthine oxidase. The generation of superoxide radicals in leaf peroxisomes together with the recently described production of these oxygen radicals in glyoxysomes suggests that O{sub 2}{sup {minus}} generation could be a common metabolic property of peroxisomes and further supports the existence of active oxygen-related roles for peroxisomes in cellular metabolism.« less

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis

PubMed Central

Kuzu, Mutlu; Niefind, Karsten; Hummel, Werner; Schomburg, Dietmar

2005-01-01

NADH oxidase (NOX) from Lactobacillus brevis is a homotetrameric flavoenzyme composed of 450 amino acids per subunit. The molecular weight of each monomer is 48.8 kDa. The enzyme catalyzes the oxidation of two equivalents of NADH and reduces one equivalent of oxygen to yield two equivalents of water, without releasing hydrogen peroxide after the reduction of the first equivalent of NADH. Crystals of this protein were grown in the presence of 34% polyethylene glycol monomethyl ether 2000, 0.1 M sodium acetate and 0.2 M ammonium sulfate at pH 5.4. They belong to the tetragonal space group P43212, with unit-cell parameters a = 74.8, b = 95.7, c = 116.9 Å, α = γ = 90, β = 103.8°. The current diffraction limit is 4.0 Å. The self-rotation function of the native data set is consistent with a NOX tetramer in the asymmetric unit. PMID:16511087

Overexpression of NADH-dependent fumarate reductase improves D-xylose fermentation in recombinant Saccharomyces cerevisiae.

PubMed

Salusjärvi, Laura; Kaunisto, Sanna; Holmström, Sami; Vehkomäki, Maija-Leena; Koivuranta, Kari; Pitkänen, Juha-Pekka; Ruohonen, Laura

2013-12-01

Deviation from optimal levels and ratios of redox cofactors NAD(H) and NADP(H) is common when microbes are metabolically engineered. The resulting redox imbalance often reduces the rate of substrate utilization as well as biomass and product formation. An example is the metabolism of D-xylose by recombinant Saccharomyces cerevisiae strains expressing xylose reductase and xylitol dehydrogenase encoding genes from Scheffersomyces stipitis. This pathway requires both NADPH and NAD(+). The effect of overexpressing the glycosomal NADH-dependent fumarate reductase (FRD) of Trypanosoma brucei in D-xylose-utilizing S. cerevisiae alone and together with an endogenous, cytosol directed NADH-kinase (POS5Δ17) was studied as one possible solution to overcome this imbalance. Expression of FRD and FRD + POS5Δ17 resulted in 60 and 23 % increase in ethanol yield, respectively, on D-xylose under anaerobic conditions. At the same time, xylitol yield decreased in the FRD strain suggesting an improvement in redox balance. We show that fumarate reductase of T. brucei can provide an important source of NAD(+) in yeast under anaerobic conditions, and can be useful for metabolic engineering strategies where the redox cofactors need to be balanced. The effects of FRD and NADH-kinase on aerobic and anaerobic D-xylose and D-glucose metabolism are discussed.

Localization of Ubiquinone-8 in the Na+-pumping NADH:Quinone Oxidoreductase from Vibrio cholerae*

PubMed Central

Casutt, Marco S.; Nedielkov, Ruslan; Wendelspiess, Severin; Vossler, Sara; Gerken, Uwe; Murai, Masatoshi; Miyoshi, Hideto; Möller, Heiko M.; Steuber, Julia

2011-01-01

Na+ is the second major coupling ion at membranes after protons, and many pathogenic bacteria use the sodium-motive force to their advantage. A prominent example is Vibrio cholerae, which relies on the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR) as the first complex in its respiratory chain. The Na+-NQR is a multisubunit, membrane-embedded NADH dehydrogenase that oxidizes NADH and reduces quinone to quinol. Existing models describing redox-driven Na+ translocation by the Na+-NQR are based on the assumption that the pump contains four flavins and one FeS cluster. Here we show that the large, peripheral NqrA subunit of the Na+-NQR binds one molecule of ubiquinone-8. Investigations of the dynamic interaction of NqrA with quinones by surface plasmon resonance and saturation transfer difference NMR reveal a high affinity, which is determined by the methoxy groups at the C-2 and C-3 positions of the quinone headgroup. Using photoactivatable quinone derivatives, it is demonstrated that ubiquinone-8 bound to NqrA occupies a functional site. A novel scheme of electron transfer in Na+-NQR is proposed that is initiated by NADH oxidation on subunit NqrF and leads to quinol formation on subunit NqrA. PMID:21885438

KENNEDY SPACE CENTER, FLA. - STS-81 Mission Specialist Jeff Wisoff prepares to enter the Space Shuttle Atlantis at Launch Pad 39B with help from White Room closeout crew members Danny Wyatt (center) and Al Rochford.

NASA Image and Video Library

1997-01-12

KENNEDY SPACE CENTER, FLA. - STS-81 Mission Specialist Jeff Wisoff prepares to enter the Space Shuttle Atlantis at Launch Pad 39B with help from White Room closeout crew members Danny Wyatt (center) and Al Rochford.

Coulometric determination of NAD+ and NADH in normal and cancer cells using LDH, RVC and a polymer mediator.

PubMed

Torabi, F; Ramanathan, K; Larsson, P O; Gorton, L; Svanberg, K; Okamoto, Y; Danielsson, B; Khayyami, M

1999-11-15

An electrochemical method for the measurement of NAD(+) and NADH in normal and cancer tissues using flow injection analysis (FIA) is reported. Reticulated vitreous carbon (RVC) electrodes with entrapped l-lactate dehydrogenase (LDH) and a new redox polymer containing covalently bound toluidine blue O (TBO) were employed for this purpose. Both NAD(+) and NADH were estimated coulometrically based on their reaction with LDH. The latter was immobilized on controlled pore glass (CPG) by cross-linking with glutaraldehyde and packed within the RVC. The concentrations of NAD(+) and NADH in the tissues, estimated using different electron mediators such as ferricyanide (FCN), meldola blue (MB) and TBO have also been compared. The effects of flow rate, pH, applied potential (versus Ag/AgCl reference) and adsorption of the mediators have also been investigated. Based on the measurements of NAD(+) and NADH in normal and cancer tissues it has been concluded that the NADH concentration is lower, while the NAD(+) concentration is higher in cancer tissues. Amongst the electron mediators TBO was found to be a more stable mediator for such measurements.

Kinetic mechanism and quaternary structure of Aminobacter aminovorans NADH:flavin oxidoreductase: an unusual flavin reductase with bound flavin.

PubMed

Russell, Thomas R; Demeler, Borries; Tu, Shiao-Chun

2004-02-17

The homodimeric NADH:flavin oxidoreductase from Aminobacter aminovorans is an NADH-specific flavin reductase herein designated FRD(Aa). FRD(Aa) was characterized with respect to purification yields, thermal stability, isoelectric point, molar absorption coefficient, and effects of phosphate buffer strength and pH on activity. Evidence from this work favors the classification of FRD(Aa) as a flavin cofactor-utilizing class I flavin reductase. The isolated native FRD(Aa) contained about 0.5 bound riboflavin-5'-phosphate (FMN) per enzyme monomer, but one bound flavin cofactor per monomer was obtainable in the presence of excess FMN or riboflavin. In addition, FRD(Aa) holoenzyme also utilized FMN, riboflavin, or FAD as a substrate. Steady-state kinetic results of substrate titrations, dead-end inhibition by AMP and lumichrome, and product inhibition by NAD(+) indicated an ordered sequential mechanism with NADH as the first binding substrate and reduced FMN as the first leaving product. This is contrary to the ping-pong mechanism shown by other class I flavin reductases. The FMN bound to the native FRD(Aa) can be fully reduced by NADH and subsequently reoxidized by oxygen. No NADH binding was detected using 90 microM FRD(Aa) apoenzyme and 300 microM NADH. All results favor the interpretation that the bound FMN was a cofactor rather than a substrate. It is highly unusual that a flavin reductase using a sequential mechanism would require a flavin cofactor to facilitate redox exchange between NADH and a flavin substrate. FRD(Aa) exhibited a monomer-dimer equilibrium with a K(d) of 2.7 microM. Similarities and differences between FRD(Aa) and certain flavin reductases are discussed.

Deployment of DRAGONSAT from Space Shuttle Endeavours Payload Bay

NASA Image and Video Library

2009-07-30

S127-E-012308 (30 July 2009) --- As seen through windows on the aft flight deck of Space Shuttle Endeavour, a Department of Defense pico-satellite known as Atmospheric Neutral Density Experiment 2 (ANDE-2) is released from the shuttle's payload bay by STS-127 crew members. ANDE-2 consists of two spherical micro-satellites which will measure the density and composition of the low-Earth orbit (LEO) atmosphere while being tracked from the ground. The data will be used to better predict the movement of objects in orbit.

Deployment of DRAGONSAT from Space Shuttle Endeavours Payload Bay

NASA Image and Video Library

2009-07-30

S127-E-012322 (30 July 2009) --- As seen through windows on the aft flight deck of Space Shuttle Endeavour, a Department of Defense pico-satellite known as Atmospheric Neutral Density Experiment 2 (ANDE-2) is released from the shuttle's payload bay by STS-127 crew members. ANDE-2 consists of two spherical micro-satellites which will measure the density and composition of the low-Earth orbit (LEO) atmosphere while being tracked from the ground. The data will be used to better predict the movement of objects in orbit.

Space Shuttle food tray

NASA Image and Video Library

1983-11-28

STS009-05-0153 (28 Nov. - 8 Dec. 1983) --- Though STS-9 was the space shuttle Columbia's sixth spaceflight, it was the first opportunity for an onboard galley, some of the results of which are shown in this 35mm scene on the flight deck. The metal tray makes for easy preparation and serving of in-space meals for crew members. This crewman is seated at the pilot's station on the flight deck. The actual galley is located in the middeck. Photo credit: NASA

A light-responsive and periodic NADH oxidase activity of the cell surface of Tetrahymena and of human buffy coat cells

NASA Technical Reports Server (NTRS)

Peter, A. D.; Morre, D. J.; Morre, D. M.

2000-01-01

Oxidation of external NADH (NADH is an impermeant substrate) by cells of Tetrahymena pyriformis oscillated with a period of 24-26 min. The period length in darkness (25.6 min) appeared to be slightly longer than the period in light (approximately 24 min). When Tetrahymena were placed in darkness for 30-50 min and then returned to light, a new maximum in the rate of NADH oxidation was observed 36-38 min (13 + 24) min after the beginning of the light treatment. The cell-surface NADH oxidase of human buffy coats (a mixture of white cells and platelets) also was periodic and light responsive.

Mutational analysis of the hyc-operon determining the relationship between hydrogenase-3 and NADH pathway in Enterobacter aerogenes.

PubMed

Pi, Jian; Jawed, Muhammad; Wang, Jun; Xu, Li; Yan, Yunjun

2016-01-01

In this study, the hydrogenase-3 gene cluster (hycDEFGH) was isolated and identified from Enterobacter aerogenes CCTCC AB91102. All gene products were highly homologous to the reported bacterial hydrogenase-3 (Hyd-3) proteins. The genes hycE, hycF, hycG encoding the subunits of hydrogenase-3 were targeted for genetic knockout to inhibit the FHL hydrogen production pathway via the Red recombination system, generating three mutant strains AB91102-E (ΔhycE), AB91102-F (ΔhycF) and AB91102-G (ΔhycG). Deletion of the three genes affected the integrity of hydrogenase-3. The hydrogen production experiments with the mutant strains showed that no hydrogen was detected compared with the wild type (0.886 mol/mol glucose), demonstrating that knocking out any of the three genes could inhibit NADH hydrogen production pathway. Meanwhile, the metabolites of the mutant strains were significantly changed in comparison with the wild type, indicating corresponding changes in metabolic flux by mutation. Additionally, the activity of NADH-mediated hydrogenase was found to be nil in the mutant strains. The chemostat experiments showed that the NADH/NAD(+) ratio of the mutant strains increased nearly 1.4-fold compared with the wild type. The NADH-mediated hydrogenase activity and NADH/NAD(+) ratio analysis both suggested that NADH pathway required the involvement of the electron transport chain of hydrogenase-3. Copyright © 2015 Elsevier Inc. All rights reserved.

Shuttle orbiter storage locker system: A study

NASA Technical Reports Server (NTRS)

Butler, D. R.; Schowalter, D. T.; Weil, D. C.

1973-01-01

Study has been made to assure maximum utility of storage space and crew member facilities in planned space shuttle orbiter. Techniques discussed in this study should be of interest to designers of storage facilities in which space is at premium and vibration is severe. Manufacturers of boats, campers, house trailers, and aircraft could benefit from it.

Removal of H2O2 and generation of superoxide radical: Role of cytochrome c and NADH

PubMed Central

Velayutham, Murugesan; Hemann, Craig; Zweier, Jay L.

2011-01-01

In cells, mitochondria, endoplasmic reticulum, and peroxisomes are the major sources of reactive oxygen species (ROS) under physiological and pathophysiological conditions. Cytochrome c (cyt c) is known to participate in mitochondrial electron transport and has antioxidant and peroxidase activities. Under oxidative or nitrative stress, the peroxidase activity of Fe3+cyt c is increased. The level of NADH is also increased under pathophysiological conditions such as ischemia and diabetes and a concurrent increase in hydrogen peroxide (H2O2) production occurs. Studies were performed to understand the related mechanisms of radical generation and NADH oxidation by Fe3+cyt c in the presence of H2O2. Electron paramagnetic resonance (EPR) spin trapping studies using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) were performed with NADH, Fe3+cyt c, and H2O2 in the presence of methyl-β-cyclodextrin. An EPR spectrum corresponding to the superoxide radical adduct of DMPO encapsulated in methyl-β-cyclodextrin was obtained. This EPR signal was quenched by the addition of the superoxide scavenging enzyme Cu,Zn-superoxide dismutase (SOD1). The amount of superoxide radical adduct formed from the oxidation of NADH by the peroxidase activity of Fe3+cyt c increased with NADH and H2O2 concentration. From these results, we propose a mechanism in which the peroxidase activity of Fe3+cyt c oxidizes NADH to NAD•, which in turn donates an electron to O2 resulting in superoxide radical formation. A UV-visible spectroscopic study shows that Fe3+cyt c is reduced in the presence of both NADH and H2O2. Our results suggest that Fe3+cyt c could have a novel role in the deleterious effects of ischemia/reperfusion and diabetes due to increased production of superoxide radical. In addition, Fe3+cyt c may play a key role in the mitochondrial “ROS-induced ROS-release (RIRR)” signaling and in mitochondrial and cellular injury/death. The increased oxidation of NADH and generation of superoxide radical

Nitrate transport is independent of NADH and NAD(P)H nitrate reductases in barley seedlings

NASA Technical Reports Server (NTRS)

Warner, R. L.; Huffaker, R. C.

1989-01-01

Barley (Hordeum vulgare L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes. Four isogenic lines with different nitrate reductase isozyme combinations were used to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation in barley seedlings. Both nitrate reductase isozymes were induced by nitrate and were required for maximum nitrate assimilation in barley seedlings. Genotypes lacking the NADH isozyme (Az12) or the NAD(P)H isozyme (Az70) assimilated 65 or 85%, respectively, as much nitrate as the wild type. Nitrate assimilation by genotype (Az12;Az70) which is deficient in both nitrate reductases, was only 13% of the wild type indicating that the NADH and NAD(P)H nitrate reductase isozymes are responsible for most of the nitrate reduction in barley seedlings. For all genotypes, nitrate assimilation rates in the dark were about 55% of the rates in light. Hypotheses that nitrate reductase has direct or indirect roles in nitrate uptake were not supported by this study. Induction of nitrate transporters and the kinetics of net nitrate uptake were the same for all four genotypes indicating that neither nitrate reductase isozyme has a direct role in nitrate uptake in barley seedlings.

Involvement of NADH Oxidase in Biofilm Formation in Streptococcus sanguinis

PubMed Central

Ge, Xiuchun; Shi, Xiaoli; Shi, Limei; Liu, Jinlin; Stone, Victoria; Kong, Fanxiang; Kitten, Todd; Xu, Ping

2016-01-01

Biofilms play important roles in microbial communities and are related to infectious diseases. Here, we report direct evidence that a bacterial nox gene encoding NADH oxidase is involved in biofilm formation. A dramatic reduction in biofilm formation was observed in a Streptococcus sanguinis nox mutant under anaerobic conditions without any decrease in growth. The membrane fluidity of the mutant bacterial cells was found to be decreased and the fatty acid composition altered, with increased palmitic acid and decreased stearic acid and vaccenic acid. Extracellular DNA of the mutant was reduced in abundance and bacterial competence was suppressed. Gene expression analysis in the mutant identified two genes with altered expression, gtfP and Idh, which were found to be related to biofilm formation through examination of their deletion mutants. NADH oxidase-related metabolic pathways were analyzed, further clarifying the function of this enzyme in biofilm formation. PMID:26950587

Studies of a Halophilic NADH Dehydrogenase. 1: Purification and Properties of the Enzyme

NASA Technical Reports Server (NTRS)

Hochstein, Lawrence I.; Dalton, Bonnie P.

1973-01-01

An NADH dehydrogenase obtained from an extremely halophilic bacterium was purified 570-fold by a combination of gel filtration, chromatography on hydroxyapatite, and ion-exchange chromatography on QAE-Sephadex. The purified enzyme appeared to be FAD-linked and bad an apparent molecular weight of 64000. Even though enzyme activity was stimulated by NaCl, considerable activity (430 % of the maximum activity observed in the presence of 2.5 M NaCl) was observed in the absence of added NaCl. The enzyme was unstable when incubated in solutions of low ionic strength. The presence of NADH enhanced the stability of the enzyme.

Space Shuttle Projects

NASA Image and Video Library

2002-03-08

After five days of service and upgrade work on the Hubble Space Telescope (HST), the STS-109 crew photographed the giant telescope in the shuttle's cargo bay. The telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm, where 4 of the 7-member crew performed 5 space walks completing system upgrades to the HST. Included in those upgrades were: The replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when its original coolant ran out. The Marshall Space Flight Center had the responsibility for the design, development, and construction of the the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. Launched March 1, 2002, the STS-109 HST servicing mission lasted 10 days, 22 hours, and 11 minutes. It was the 108th flight overall in NASA's Space Shuttle Program.

Space Shuttle Projects

NASA Image and Video Library

2002-03-11

On the Space Shuttle Columbia's mid deck, the STS-109 crew of seven pose for the traditional in-flight portrait. From the left (front row), are astronauts Nancy J. Currie, mission specialist; Scott D. Altman, mission commander; and Duane G. Carey, pilot. Pictured on the back row from left to right are astronauts John M. Grunsfield, payload commander; and Richard M. Lirneham, James H. Newman, and Michael J. Massimino, all mission specialists. The 108th flight overall in NASA's Space Shuttle Program, the STS-109 mission launched March 1, 2002, and lasted 10 days, 22 hours, and 11 minutes. The goal of the mission was the maintenance and upgrade of the Hubble Space Telescope (HST). Using Columbia's robotic arm, the telescope was captured and secured on a work stand in Columbia's payload bay where four members of the crew performed five space walks to complete system upgrades to the HST. The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit.

Polarized fluorescence in NADH under two-photon excitation with femtosecond laser pulses

NASA Astrophysics Data System (ADS)

Vasyutinskii, O. S.; Smolin, A. G.; Oswald, C.; Gericke, K. H.

2017-04-01

Polarized fluorescence decay in NADH molecules in aqueous solution under two-photon excitation by femtosecond laser pulses has been studied. The excitation was carried out by linear and circularly polarized radiation at four wavelengths: 720, 730, 740, and 750 nm. Time-dependent polarized fluorescence signals were recorded as a function of the excitation light polarization and used for determination of a set of molecular parameters, two lifetimes characterizing the molecular excited states, and the rotation correlation time τrot. The results obtained can be used to create and prove theoretical models describing the intensity and polarization of fluorescence in NADH involved in the regulation of the redox reactions in cells and tissues of living organisms.

Stereospecificity of NAD+/NADH Reactions: A Project Experiment for Advanced Undergraduates.

ERIC Educational Resources Information Center

Lowrey, Jonathan S.; And Others

1981-01-01

Presents background information, materials needed, and experimental procedures to study enzymes dependent on pyridine nucleotide coenzymes (NAD/NADH). The experiments, suitable for advanced organic or biochemistry courses, require approximately 10-15 hours to complete. (SK)

Space Shuttle Projects

NASA Image and Video Library

1992-05-14

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. This onboard photo captures the free flying INTELSAT IV.

Space Shuttle Projects

NASA Image and Video Library

1994-10-08

Designed by the crew members, the STS-63 crew patch depicts the orbiter maneuvering to rendezvous with Russia's Space Station Mir. The name is printed in Cyrillic on the side of the station. Visible in the Orbiter's payload bay are the commercial space laboratory Spacehab and the Shuttle Pointed Autonomous Research Tool for Astronomy (SPARTAN) satellite which are major payloads on the flight. The six points on the rising sun and the three stars are symbolic of the mission's Space Transportation System (STS) numerical designation. Flags of the United States and Russia at the bottom of the patch symbolize the cooperative operations of this mission.

Space Shuttle Projects

NASA Image and Video Library

1992-05-14

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3) which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. In this STS-49 onboard photo, Astronaut Kathryn Thornton joins three struts together during her Extra Vehicular Activity (EVA).

Comparison of oral nicotinamide adenine dinucleotide (NADH) versus conventional therapy for chronic fatigue syndrome.

PubMed

Santaella, María L; Font, Ivonne; Disdier, Orville M

2004-06-01

To compare effectiveness of oral therapy with reduced nicotinamide adenine dinucleotide (NADH) to conventional modalities of treatment in patients with chronic fatigue syndrome (CFS). CFS is a potentially disabling condition of unknown etiology. Although its clinical presentation is associated to a myriad of symptoms, fatigue is a universal and essential finding for its diagnosis. No therapeutic regimen has proven effective for this condition. A total of 31 patients fulfilling the Centers for Disease Control criteria for CFS, were randomly assigned to either NADH or nutritional supplements and psychological therapy for 24 months. A thorough medical history, physical examination and completion of a questionnaire on the severity of fatigue and other symptoms were performed each trimester of therapy. In addition, all of them underwent evaluation in terms of immunological parameters and viral antibody titers. Statistical analysis was applied to the demographic data, as well as to symptoms scores at baseline and at each trimester of therapy. The twelve patients who received NADH had a dramatic and statistically significant reduction of the mean symptom score in the first trimester (p < 0.001). However, symptom scores in the subsequent trimesters of therapy were similar in both treatment groups. Elevated IgG and Ig E antibody levels were found in a significant number of patients. Observed effectiveness of NADH over conventional treatment in the first trimester of the trial and the trend of improvement of that modality in the subsequent trimesters should be further assessed in a larger patient sample.

STS-71 Shuttle Atlantis landing closeup

NASA Technical Reports Server (NTRS)

1995-01-01

KENNEDY SPACE CENTER, FLA. -- The Space Shuttle orbiter Atlantis makes a smooth touchdown on Runway 15 of the Shuttle Landing Facility, bringing an end to the historic STS-71 mission which featured the first docking between the Space Shuttle and the Russian Mir space station. The chase plane, the Shuttle Training Aircraft flown by Robert D. Cabana, head of the Astronaut Office, is in the upper left of photo. Main gear touchdown of Atlantis was at 10:54:34 a.m. EDT, on July 7, 1995. This was the first of seven scheduled Shuttle/Mir docking missions. The 10-day mission also set the record for having the most people who have flown in an orbiter during a mission: the five U.S. astronauts and two cosmonauts who were launched on Atlantis on June 27, and three space flyers who have been aboard Mir since March 16 and were returned to Earth in Atlantis. The STS-71 crew included Mission Commander Robert L. 'Hoot' Gibson, Pilot Charles J. Precourt, Payload Commander Dr. Ellen S. Baker, and Mission Specialists Bonnie J. Dunbar and Gregory J. Harbaugh. Also part of the STS-71 crew were two cosmonauts who comprise the Mir 19 crew -- Mission Commander Anatoly Y. Solovyev and Flight Engineer Nikolai M. Budarin. They transfered to Mir during the four days of docking operations, and remain there. They replaced the Mir 18 crew of U.S. astronaut and cosmonaut researcher Dr. Norman E. Thagard, and cosmonauts Vladimir N. Dezhurov, who served as mission commander, and Gennadiy M. Strekalov, who served as flight engineer. The Mir crew joined the American STS-71 crew members for the return to Earth on Atlantis.

Metabolism of hydroxypyruvate in a mutant of barley lacking NADH-dependent hydroxypyruvate reductase, an important photorespiratory enzyme activity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murray, A.J.S.; Blackwell, R.D.; Lea, P.J.

1989-09-01

A mutant of barley (Hordeum vulgare L.), LaPr 88/29, deficient in NADH-dependent hydroxypyruvate reductase (HPR) activity has been isolated. The activities of both NADH (5%) and NADPH-dependent (19%) HPR were severely reduced in this mutant compared to the wild type. Although lacking an enzyme in the main carbon pathway of photorespiration, this mutant was capable of CO{sub 2} fixation rates equivalent to 75% of that of the wild type, in normal atmospheres and 50% O{sub 2}. There also appeared to be little disruption to the photorespiratory metabolism as ammonia release, CO{sub 2} efflux and {sup 14}CO{sub 2} release from L-(U-{supmore » 14}C)serine feeding were similar in both mutant and wild-type leaves. When leaves of LaPr 88/29 were fed either ({sup 14}C)serine or {sup 14}CO{sub 2}, the accumulation of radioactivity was in serine and not in hydroxypyruvate, although the mutant was still able to metabolize over 25% of the supplied ({sup 14}C)serine into sucrose. After 3 hours in air the soluble amino acid pool was almost totally dominated by serine and glycine. LaPr 88/29 has also been used to show that NADH-glyoxylate reductase and NADH-HPR are probably not catalyzed by the same enzyme in barley and that over 80% of the NADPH-dependent HPR activity is due to the NADH-dependent enzyme. We also suggest that the alternative NADPH activity can metabolize a proportion, but not all, of the hydroxypyruvate produced during photorespiration and may thus form a useful backup to the NADH-dependent enzyme under conditions of maximal photorespiration.« less

Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri.

PubMed

Li, Fuli; Hinderberger, Julia; Seedorf, Henning; Zhang, Jin; Buckel, Wolfgang; Thauer, Rudolf K

2008-02-01

Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously observed is due to the fact that the cell extracts catalyze the reduction of acetyl-CoA with NADH via crotonyl-CoA to butyryl-CoA. The cytoplasmic butyryl-CoA dehydrogenase complex was purified and is shown to couple the endergonic reduction of ferredoxin (E0' = -410 mV) with NADH (E0' = -320 mV) to the exergonic reduction of crotonyl-CoA to butyryl-CoA (E0' = -10 mV) with NADH. The stoichiometry of the fully coupled reaction is extrapolated to be as follows: 2 NADH + 1 oxidized ferredoxin + 1 crotonyl-CoA = 2 NAD+ + 1 ferredoxin reduced by two electrons + 1 butyryl-CoA. The implications of this finding for the energy metabolism of butyrate-forming anaerobes are discussed in the accompanying paper.

In vitro assessment of anticholinesterase and NADH oxidase inhibitory activities of an edible fern, Diplazium esculentum.

PubMed

Roy, Subhrajyoti; Dutta, Somit; Chaudhuri, Tapas Kumar

2015-07-01

Diplazium esculentum is the most commonly consumed edible fern throughout Asia and Oceania. Several studies have been performed so far to determine different functional properties of this plant, but there have been no reports on the anticholinesterase and nicotinamide adenine dinucleotide (NADH) oxidase inhibitory activities of this plant. Therefore, the present study was conducted to determine the anticholinesterase and NADH oxidase inhibitory activities of 70% methanolic extract of D. esculentum. The D. esculentum extract was investigated for its acetylcholinesterase and NADH oxidase inhibitory activities as well as its free radical scavenging and total antioxidant activities in the linoleic acid system. The free radical scavenging activity of the extract was determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method. The total antioxidant activity of the extract was evaluated by ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. The D. esculentum extract inhibited acetylcholinesterase and NADH oxidase in a dose-dependent manner, with IC50 values of 272.97±19.38 and 265.81±21.20 μg/mL, respectively. The extract also showed a potent DPPH radical scavenging activity with an IC50 value of 402.88±12.70 μg/mL. Moreover, the extract showed 27.41% and 33.22% of total antioxidant activities determined by FTC and TBA methods, respectively. Results indicated that 70% methanolic extract of D. esculentum effectively inhibited the enzymes acetylcholinesterase and NADH oxidase and acted as a potent antioxidant and free radical scavenger. These in vitro assays indicate that this plant extract is a significant source of natural antioxidants, which may be helpful in preventing the progression of various neurodegenerative disorders associated with oxidative stress.

Nitrate Transport Is Independent of NADH and NAD(P)H Nitrate Reductases in Barley Seedlings 1

PubMed Central

Warner, Robert L.; Huffaker, Ray C.

1989-01-01

Barley (Hordeum vulgare L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes. Four isogenic lines with different nitrate reductase isozyme combinations were used to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation in barley seedlings. Both nitrate reductase isozymes were induced by nitrate and were required for maximum nitrate assimilation in barley seedlings. Genotypes lacking the NADH isozyme (Az12) or the NAD(P)H isozyme (Az70) assimilated 65 or 85%, respectively, as much nitrate as the wild type. Nitrate assimilation by genotype (Az12;Az70) which is deficient in both nitrate reductases, was only 13% of the wild type indicating that the NADH and NAD(P)H nitrate reductase isozymes are responsible for most of the nitrate reduction in barley seedlings. For all genotypes, nitrate assimilation rates in the dark were about 55% of the rates in light. Hypotheses that nitrate reductase has direct or indirect roles in nitrate uptake were not supported by this study. Induction of nitrate transporters and the kinetics of net nitrate uptake were the same for all four genotypes indicating that neither nitrate reductase isozyme has a direct role in nitrate uptake in barley seedlings. PMID:11537465

Lambda Red-mediated mutagenesis and efficient large scale affinity purification of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I).

PubMed

Pohl, Thomas; Uhlmann, Mareike; Kaufenstein, Miriam; Friedrich, Thorsten

2007-09-18

The proton-pumping NADH:ubiquinone oxidoreductase, the respiratory complex I, couples the transfer of electrons from NADH to ubiquinone with the translocation of protons across the membrane. The Escherichia coli complex I consists of 13 different subunits named NuoA-N (from NADH:ubiquinone oxidoreductase), that are coded by the genes of the nuo-operon. Genetic manipulation of the operon is difficult due to its enormous size. The enzymatic activity of variants is obscured by an alternative NADH dehydrogenase, and purification of the variants is hampered by their instability. To overcome these problems the entire E. coli nuo-operon was cloned and placed under control of the l-arabinose inducible promoter ParaBAD. The exposed N-terminus of subunit NuoF was chosen for engineering the complex with a hexahistidine-tag by lambda-Red-mediated recombineering. Overproduction of the complex from this construct in a strain which is devoid of any membrane-bound NADH dehydrogenase led to the assembly of a catalytically active complex causing the entire NADH oxidase activity of the cytoplasmic membranes. After solubilization with dodecyl maltoside the engineered complex binds to a Ni2+-iminodiacetic acid matrix allowing the purification of approximately 11 mg of complex I from 25 g of cells. The preparation is pure and monodisperse and comprises all known subunits and cofactors. It contains more lipids than earlier preparations due to the gentle and fast purification procedure. After reconstitution in proteoliposomes it couples the electron transfer with proton translocation in an inhibitor sensitive manner, thus meeting all prerequisites for structural and functional studies.

Space Shuttle Projects

NASA Image and Video Library

2001-08-01

This is the insignia of the STS-109 Space Shuttle mission. Carrying a crew of seven, the Space Shuttle Orbiter Columbia was launched with goals of maintenance and upgrades to the Hubble Space Telescope (HST). The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. The HST detects objects 25 times fainter than the dimmest objects seen from Earth and provides astronomers with an observable universe 250 times larger than is visible from ground-based telescopes, perhaps as far away as 14 billion light-years. The HST views galaxies, stars, planets, comets, possibly other solar systems, and even unusual phenomena such as quasars, with 10 times the clarity of ground-based telescopes. During the STS-109 mission, the telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm where four members of the crew performed five spacewalks completing system upgrades to the HST. Included in those upgrades were: The replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when it original coolant ran out. Lasting 10 days, 22 hours, and 11 minutes, the STS-109 mission was the 27th flight of the Orbiter Columbia and the 108th flight overall in NASA's Space Shuttle Program.

View of the shuttle Discovery STS 51-D launch

NASA Image and Video Library

1985-04-12

51D-9092 (12 April 1985) --- The Space Shuttle Discovery ascends the launch complex in Florida and heads through Atlantic skies toward its 51-D mission. The seven member crew lifted off at 8:59 a.m. (EST), April 12, 1985. This picture was made with a 35mm camera.

Space Shuttle Projects

NASA Image and Video Library

1992-05-13

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. In this onboard photo, astronauts Hieb, Akers, and Thuot have handholds on the satellite.

Space Shuttle Projects

NASA Image and Video Library

1992-05-14

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. A view through Endeavour’s busy airlock reveals astronauts Thomas Akers and Kathryn Thornton.

Bioelectrocatalytic NAD+/NADH inter-conversion: transformation of an enzymatic fuel cell into an enzymatic redox flow battery.

PubMed

Quah, Timothy; Milton, Ross D; Abdellaoui, Sofiene; Minteer, Shelley D

2017-07-25

Diaphorase and a benzylpropylviologen redox polymer were combined to create a bioelectrode that can both oxidize NADH and reduce NAD + . We demonstrate how bioelectrocatalytic NAD + /NADH inter-conversion can transform a glucose/O 2 enzymatic fuel cell (EFC) with an open circuit potential (OCP) of 1.1 V into an enzymatic redox flow battery (ERFB), which can be rapidly recharged by operation as an EFC.

Crystallization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae

PubMed Central

Casutt, Marco S.; Wendelspiess, Severin; Steuber, Julia; Fritz, Günter

2010-01-01

The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from the human pathogen Vibrio cholerae couples the exergonic oxidation of NADH by membrane-bound quinone to Na+ translocation across the membrane. Na+-NQR consists of six different subunits (NqrA–NqrF) and contains a [2Fe–2S] cluster, a noncovalently bound FAD, a noncovalently bound riboflavin, two covalently bound FMNs and potentially Q8 as cofactors. Initial crystallization of the entire Na+-NQR complex was achieved by the sitting-drop method using a nanolitre dispenser. Optimization of the crystallization conditions yielded flat yellow-coloured crystals with dimensions of up to 200 × 80 × 20 µm. The crystals diffracted to 4.0 Å resolution and belonged to space group P21, with unit-cell parameters a = 94, b = 146, c = 105 Å, α = γ = 90, β = 111°. PMID:21139223

A New Insight into the Mechanism of NADH Model Oxidation by Metal Ions in Non-Alkaline Media.

PubMed

Yang, Jin-Dong; Chen, Bao-Long; Zhu, Xiao-Qing

2018-06-11

For a long time, it has been controversial that the three-step (e-H+-e) or two-step (e-H•) mechanism was used for the oxidations of NADH and its models by metal ions in non-alkaline media. The latter mechanism has been accepted by the majority of researchers. In this work, 1-benzyl-1,4-dihydronicotinamide (BNAH) and 1-phenyl-l,4-dihydronicotinamide (PNAH) are used as NADH models, and ferrocenium (Fc+) metal ion as an electron acceptor. The kinetics for oxidations of the NADH models by Fc+ in pure acetonitrile were monitored by using UV-Vis absorption and quadratic relationship between of kobs and the concentrations of NADH models were found for the first time. The rate expression of the reactions developed according to the three-step mechanism is quite consistent with the quadratic curves. The rate constants, thermodynamic driving forces and KIEs of each elementary step for the reactions were estimated. All the results supported the three-step mechanism. The intrinsic kinetic barriers of the proton transfer from BNAH+• to BNAH and the hydrogen atom transfer from BNAH+• to BNAH+• were estimated, the results showed that the former is 11.8 kcal/mol, and the latter is larger than 24.3 kcal/mol. It is the large intrinsic kinetic barrier of the hydrogen atom transfer that makes the reactions choose the three-step rather than two-step mechanism. Further investigation of the factors affecting the intrinsic kinetic barrier of chemical reactions indicated that the large intrinsic kinetic barrier of the hydrogen atom transfer originated from the repulsion of positive charges between BNAH+• and BNAH+•. The greatest contribution of this work is the discovery of the quadratic dependence of kobs on the concentrations of the NADH models, which is inconsistent with the conventional viewpoint of the "two-step mechanism" on the oxidations of NADH and its models by metal ions in the non-alkaline media.

Space Shuttle Projects

NASA Image and Video Library

1999-11-30

These five STS-97 crew members posed for a traditional portrait during training. On the front row, left to right, are astronauts Michael J. Bloomfield, pilot; Marc Garneau, mission specialist representing the Canadian Space Agency (CSA); and Brent W. Jett, Jr., commander. In the rear, wearing training versions of the extravehicular mobility unit (EMU) space suits, (left to right) are astronauts Carlos I. Noriega, and Joseph R. Tarner, both mission specialists. The primary objective of the STS-97 mission was the delivery, assembly, and activation of the U.S. electrical power system onboard the International Space Station (ISS). The electrical power system, which is built into a 73-meter (240-foot) long solar array structure consists of solar arrays, radiators, batteries, and electronics. The entire 15.4-metric ton (17-ton) package is called the P6 Integrated Truss Segment and is the heaviest and largest element yet delivered to the station aboard a space shuttle. The electrical system will eventually provide the power necessary for the first ISS crews to live and work in the U.S. segment. The STS-97 crew of five launched aboard the Space Shuttle Orbiter Endeavor on November 30, 2000 for an 11 day mission.

Space Shuttle Projects

NASA Image and Video Library

1994-07-20

The STS-64 patch depicts the Space Shuttle Discovery in a payload-bay-to-Earth attitude with its primary payload, Lidar In-Space Technology Experiment (LITE-1) operating in support of Mission to Planet Earth. LITE-1 is a lidar system that uses a three-wavelength laser, symbolized by the three gold rays emanating from the star in the payload bay that form part of the astronaut symbol. The major objective of the LITE-1 is to gather data about the Earth's troposphere and stratosphere, represented by the clouds and dual-colored Earth limb. A secondary payload on STS-64 is the free-flier SPARTAN 201 satellite shown on the Remote Manipulator System (RMS) arm post-retrieval. The RMS also operated another payload, Shuttle Plume Impingement Flight Experiment (SPIFEX). A newly tested extravehicular activity (EVA) maneuvering device, Simplified Aid for EVA Rescue (SAFER), represented symbolically by the two small nozzles on the backpacks of the two untethered EVA crew men. The names of the crew members encircle the patch: Astronauts Richard N. Richards, L. Blaine Hammond, Jr., Jerry M. Linenger, Susan J. Helms, Carl J. Meade and Mark C. Lee. The gold or silver stars by each name represent that person's parent service.

Auxin-activated NADH oxidase activity of soybean plasma membranes is distinct from the constitutive plasma membrane NADH oxidase and exhibits prion-like properties

NASA Technical Reports Server (NTRS)

Morre, D. James; Morre, Dorothy M.; Ternes, Philipp

2003-01-01

The hormone-stimulated and growth-related cell surface hydroquinone (NADH) oxidase activity of etiolated hypocotyls of soybeans oscillates with a period of about 24 min or 60 times per 24-h day. Plasma membranes of soybean hypocotyls contain two such NADH oxidase activities that have been resolved by purification on concanavalin A columns. One in the apparent molecular weight range of 14-17 kDa is stimulated by the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The other is larger and unaffected by 2,4-D. The 2,4-D-stimulated activity absolutely requires 2,4-D for activity and exhibits a period length of about 24 min. Also exhibiting 24-min oscillations is the rate of cell enlargement induced by the addition of 2,4-D or the natural auxin indole-3-acetic acid (IAA). Immediately following 2,4-D or IAA addition, a very complex pattern of oscillations is frequently observed. However, after several hours a dominant 24-min period emerges at the expense of the constitutive activity. A recruitment process analogous to that exhibited by prions is postulated to explain this behavior.

Space Shuttle Projects

NASA Image and Video Library

1993-10-01

Designed by the mission crew members, the STS-61 crew insignia depicts the astronaut symbol superimposed against the sky with the Earth underneath. Also seen are two circles representing the optical configuration of the Hubble Space Telescope (HST). Light is focused by reflections from a large primary mirror and a smaller secondary mirror. The light is analyzed by various instruments and, according to the crew members, brings to us on Earth knowledge about planets, stars, galaxies and other celestial objects, allowing us to better understand the complex physical processes at work in the universe. The Space Shuttle Endeavour is also represented as the fundamental tool that allows the crew to perform the first servicing of the Hubble Space Telescope so its scientific deep space mission may be extended for several years to come. The overall design of the emblem, with lines converging to a high point, is also a symbolic representation of the large-scale Earth-based effort which involves space agencies, industry, and the universities to reach goals of knowledge and perfection.

Preferential inhibition of the plasma membrane NADH oxidase (NOX) activity by diphenyleneiodonium chloride with NADPH as donor

NASA Technical Reports Server (NTRS)

Morre, D. James

2002-01-01

The cell-surface NADH oxidase (NOX) protein of plant and animal cells will utilize both NADH and NADPH as reduced electron donors for activity. The two activities are distinguished by a differential inhibition by the redox inhibitor diphenyleneiodonium chloride (DPI). Using both plasma membranes and cells, activity with NADPH as donor was markedly inhibited by DPI at submicromolar concentrations, whereas with NADH as donor, DPI was much less effective or had no effect on the activity. The possibility of the inhibition being the result of two different enzymes was eliminated by the use of a recombinant NOX protein. The findings support the concept that NOX proteins serve as terminal oxidases for plasma membrane electron transport involving cytosolic reduced pyridine nucleotides as the natural electron donors and with molecular oxygen as the electron acceptor.

The steady-state kinetics of the NADH-dependent nitrite reductase from Escherichia coli K 12. Nitrite and hydroxylamine reduction.

PubMed Central

Jackson, R H; Cole, J A; Cornish-Bowden, A

1981-01-01

The reduction of both NO2- and hydroxylamine by the NADH-dependent nitrite reductase of Escherichia coli K 12 (EC 1.6.6.4) appears to follow Michaelis-Menten kinetics over a wide range of NADH concentrations. Substrate inhibition can, however, be detected at low concentrations of the product NAD+. In addition, NAD+ displays mixed product inhibition with respect to NADH and mixed or uncompetitive inhibition with respect to hydroxylamine. These inhibition characteristics are consistent with a mechanism in which hydroxylamine binds during catalysis to a different enzyme form from that generated when NAD+ is released. The apparent maximum velocity with NADH as varied substrate increases as the NAD+ concentration increases from 0.05 to 0.7 mM with 1 mM-NO2- or 100 mM-hydroxylamine as oxidized substrate. This increase is more marked for hydroxylamine reduction than for NO2- reduction. Models incorporating only one binding site for NAD can account for the variation in the Michaelis-Menten parameters for both NADH and hydroxylamine with [NAD+] for hydroxylamine reduction. According to these models, activation of the reaction occurs by reversal of an over-reduction of the enzyme by NADH. If the observed activation of the enzyme by NAD+ derives both from activation of the generation of the enzyme-hydroxylamine complex from the enzyme-NO2- complex during NO2- reduction and from activation of the reduction of the enzyme-hydroxylamine complex to form NH4+, then the variation of Vapp. for NO2- or hydroxylamine with [NAD+] is consistent with the occurrence of the same enzyme-hydroxylamine complex as an intermediate in both reactions. PMID:6279095

Shuttle mission simulator requirements report, volume 1, revision A

NASA Technical Reports Server (NTRS)

Burke, J. F.

1973-01-01

The tasks are defined required to design, develop produce, and field support a shuttle mission simulator for training crew members and ground support personnel. The requirements for program management, control, systems engineering, design and development are discussed along with the design and construction standards, software design, control and display, communication and tracking, and systems integration.

Dual utilization of NADPH and NADH cofactors enhances xylitol production in engineered Saccharomyces cerevisiae.

PubMed

Jo, Jung-Hyun; Oh, Sun-Young; Lee, Hyeun-Soo; Park, Yong-Cheol; Seo, Jin-Ho

2015-12-01

Xylitol, a natural sweetener, can be produced by hydrogenation of xylose in hemicelluloses. In microbial processes, utilization of only NADPH cofactor limited commercialization of xylitol biosynthesis. To overcome this drawback, Saccharomyces cerevisiae D452-2 was engineered to express two types of xylose reductase (XR) with either NADPH-dependence or NADH-preference. Engineered S. cerevisiae DWM expressing both the XRs exhibited higher xylitol productivity than the yeast strain expressing NADPH-dependent XR only (DWW) in both batch and glucose-limited fed-batch cultures. Furthermore, the coexpression of S. cerevisiae ZWF1 and ACS1 genes in the DWM strain increased intracellular concentrations of NADPH and NADH and improved maximum xylitol productivity by 17%, relative to that for the DWM strain. Finally, the optimized fed-batch fermentation of S. cerevisiae DWM-ZWF1-ACS1 resulted in 196.2 g/L xylitol concentration, 4.27 g/L h productivity and almost the theoretical yield. Expression of the two types of XR utilizing both NADPH and NADH is a promising strategy to meet the industrial demands for microbial xylitol production. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Inhibition of mitochondrial calcium efflux by clonazepam in intact single rat cardiomyocytes and effects on NADH production.

PubMed

Griffiths, E J; Wei, S K; Haigney, M C; Ocampo, C J; Stern, M D; Silverman, H S

1997-04-01

The aims of this study were to determine: (i) whether clonazepam and CGP37157, which inhibit the Na+/Ca2+ exchanger of isolated mitochondria, could inhibit mitochondrial Ca2+ efflux in intact cells; and (ii) whether any sustained increase in mitochondrial [Ca2+] ([Ca2+]m) could alter mitochondrial NADH levels. [Ca2+]m was measured in Indo-1/AM loaded rat ventricular myocytes where the cytosolic fluorescence signal was quenched by superfusion with Mn2+. NADH levels were determined from cell autofluorescence. Upon exposure of myocytes to 50 nM norepinephrine (NE) and a stimulation rate of 3 Hz, [Ca2+]m increased from 59 +/- 3 nM to a peak of 517 +/- 115 nM (n = 8) which recovered rapidly upon return to low stimulation rate (0.2 Hz) and washout of NE. In the presence of clonazepam, the peak increase in [Ca2+]m was 937 +/- 192 nM (n = 5) which remained elevated at 652 +/- 131 nM upon removal of the stimulus. CGP37157 in some cells did give the same inhibition of mitochondrial Ca2+ efflux as clonazepam, but the effect was inconsistent since not all cells were capable of following the stimulation rate in presence of this compound. NADH levels increased upon exposure to rapid stimulation in the presence of NE alone and recovered upon return to low stimulation rates, whereas in clonazepam treated cells the recovery of NADH was prevented. We conclude that clonazepam is an effective inhibitor of mitochondrial [Ca2+] efflux in intact cells and also maintains the increase in NADH levels which occurs upon rapid stimulation of cells.

SPACE SHUTTLE MISSION 41C - OFFICIAL CREW INSIGNIA

NASA Image and Video Library

1984-01-01

S84-25522 (15 Jan. 1984) --- The patch to be worn by the five members of NASA's STS-41C space mission tells the story of that flight. It features a helmet visor of an astronaut performing an extravehicular activity (EVA). In the visor are reflected the sun's rays, the space shuttle Challenger and its Remote Manipulator System (RMS) deploying the Long Duration Exposure Facility (LDEF), Earth and blue sky, and another astronaut working at the damaged Solar Maximum Satellite (SMS). The scene is encircled by the surnames of the crew members. They are astronauts Robert L. Crippen, commander; Francis R. (Dick) Scobee, pilot; and Terry J. Hart, James D. van Hoften and George D. Nelson, all mission specialists. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

Increased Production of Hydrogen Peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon Aeration: Involvement of an NADH Oxidase in Oxidative Stress

PubMed Central

Marty-Teysset, C.; de la Torre, F.; Garel, J.-R.

2000-01-01

The growth of Lactobacillus delbrueckii subsp. bulgaricus (L. delbrueckii subsp. bulgaricus) on lactose was altered upon aerating the cultures by agitation. Aeration caused the bacteria to enter early into stationary phase, thus reducing markedly the biomass production but without modifying the maximum growth rate. The early entry into stationary phase of aerated cultures was probably related to the accumulation of hydrogen peroxide in the medium. Indeed, the concentration of hydrogen peroxide in aerated cultures was two to three times higher than in unaerated ones. Also, a similar shift from exponential to stationary phase could be induced in unaerated cultures by adding increasing concentrations of hydrogen peroxide. A significant fraction of the hydrogen peroxide produced by L. delbrueckii subsp. bulgaricus originated from the reduction of molecular oxygen by NADH catalyzed by an NADH:H2O2 oxidase. The specific activity of this NADH oxidase was the same in aerated and unaerated cultures, suggesting that the amount of this enzyme was not directly regulated by oxygen. Aeration did not change the homolactic character of lactose fermentation by L. delbrueckii subsp. bulgaricus and most of the NADH was reoxidized by lactate dehydrogenase with pyruvate. This indicated that NADH oxidase had no (or a very small) energetic role and could be involved in eliminating oxygen. PMID:10618234

Non-invasive In-cell Determination of Free Cytosolic [NAD+]/[NADH] Ratios Using Hyperpolarized Glucose Show Large Variations in Metabolic Phenotypes*

PubMed Central

Christensen, Caspar Elo; Karlsson, Magnus; Winther, Jakob R.; Jensen, Pernille Rose; Lerche, Mathilde H.

2014-01-01

Accumulating evidence suggest that the pyridine nucleotide NAD has far wider biological functions than its classical role in energy metabolism. NAD is used by hundreds of enzymes that catalyze substrate oxidation and, as such, it plays a key role in various biological processes such as aging, cell death, and oxidative stress. It has been suggested that changes in the ratio of free cytosolic [NAD+]/[NADH] reflects metabolic alterations leading to, or correlating with, pathological states. We have designed an isotopically labeled metabolic bioprobe of free cytosolic [NAD+]/[NADH] by combining a magnetic enhancement technique (hyperpolarization) with cellular glycolytic activity. The bioprobe reports free cytosolic [NAD+]/[NADH] ratios based on dynamically measured in-cell [pyruvate]/[lactate] ratios. We demonstrate its utility in breast and prostate cancer cells. The free cytosolic [NAD+]/[NADH] ratio determined in prostate cancer cells was 4 times higher than in breast cancer cells. This higher ratio reflects a distinct metabolic phenotype of prostate cancer cells consistent with previously reported alterations in the energy metabolism of these cells. As a reporter on free cytosolic [NAD+]/[NADH] ratio, the bioprobe will enable better understanding of the origin of diverse pathological states of the cell as well as monitor cellular consequences of diseases and/or treatments. PMID:24302737

Single-walled carbon nanotubes covalently functionalized with polytyrosine: A new material for the development of NADH-based biosensors.

PubMed

Eguílaz, Marcos; Gutierrez, Fabiana; González-Domínguez, Jose Miguel; Martínez, María T; Rivas, Gustavo

2016-12-15

We report for the first time the use of single-walled carbon nanotubes (SWCNT) covalently functionalized with polytyrosine (Polytyr) (SWCNT-Polytyr) as a new electrode material for the development of nicotinamide adenine dinucleotide (NADH)-based biosensors. The oxidation of glassy carbon electrodes (GCE) modified with SWCNT-Polytyr at potentials high enough to oxidize the tyrosine residues have allowed the electrooxidation of NADH at low potentials due to the catalytic activity of the quinones generated from the primary oxidation of tyrosine without any additional redox mediator. The amperometric detection of NADH at 0.200V showed a sensitivity of (217±3)µAmM(-1)cm(-2) and a detection limit of 7.9nM. The excellent electrocatalytic activity of SWCNT-Polytyr towards NADH oxidation has also made possible the development of a sensitive ethanol biosensor through the immobilization of alcohol dehydrogenase (ADH) via Nafion entrapment, with excellent analytical characteristics (sensitivity of (5.8±0.1)µAmM(-1)cm(-2), detection limit of 0.67µM) and very successful application for the quantification of ethanol in different commercial beverages. Copyright © 2016 Elsevier B.V. All rights reserved.

Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveri▿ †

PubMed Central

Li, Fuli; Hinderberger, Julia; Seedorf, Henning; Zhang, Jin; Buckel, Wolfgang; Thauer, Rudolf K.

2008-01-01

Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously observed is due to the fact that the cell extracts catalyze the reduction of acetyl-CoA with NADH via crotonyl-CoA to butyryl-CoA. The cytoplasmic butyryl-CoA dehydrogenase complex was purified and is shown to couple the endergonic reduction of ferredoxin (E0′ = −410 mV) with NADH (E0′ = −320 mV) to the exergonic reduction of crotonyl-CoA to butyryl-CoA (E0′ = −10 mV) with NADH. The stoichiometry of the fully coupled reaction is extrapolated to be as follows: 2 NADH + 1 oxidized ferredoxin + 1 crotonyl-CoA = 2 NAD+ + 1 ferredoxin reduced by two electrons + 1 butyryl-CoA. The implications of this finding for the energy metabolism of butyrate-forming anaerobes are discussed in the accompanying paper. PMID:17993531

Space Shuttle Projects

NASA Image and Video Library

1992-05-14

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. In this onboard photo, astronaut Kathryn Thornton is working on the Assembly of Station by EVA Methods (ASEM) in the cargo bay.

Space Shuttle Projects

NASA Image and Video Library

1989-12-05

The mission insignia for NASA's STS-31 mission features the Hubble Space Telescope (HST) in its observing configuration against a background of the universe it will study. The cosmos includes a stylistic depiction of galaxies in recognition of the contribution made by Sir Edwin Hubble to our understanding of the nature of galaxies and the expansion of the universe. The STS-31 crew points out that is it in honor of Hubble's work that this great observatory in space bears his name. The depicted Space Shuttle trails a spectrum symbolic of both the red shift observations that were so important to Hubble's work and new information which will be obtained with the HST. Encircling the art work, designed by the crew, are the names of its members.

Space Shuttle Projects

NASA Image and Video Library

1992-05-14

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. After securing the satellite with the Remote Manipulator System (RMS), the crew proceeded with preparing the satellite for its release into space.

Effect of micromolar Ca2+ on NADH inhibition of bovine kidney alpha-ketoglutarate dehydrogenase complex and possible role of Ca2+ in signal amplification.

PubMed

Lawlis, V B; Roche, T E

1980-11-20

NADH inhibition of bovine kidney alpha-ketoglutarate dehydrogenase complex was compared at 10 microM free Ca2+ or in the absence of Ca2+ (i.e., less than 1.0 nM free Ca2+). In the presence of Ca2+, NADH inhibition was appreciably decreased for a wide range of NADH:NAD+ ratios. A half-maximal decrease in NADH inhibition occurred at slightly less than 1 microM free Ca/+ (as determined with EGTA-Ca buffers). Of necessity this was observed on top of an effect of Ca2+ on the S0.5 for alpha-ketoglutarate which was decreased by Ca2+ with a half-maximal effect at a similar concentration. The effect of Ca2+ on NADH inhibition was not observed in assays of the dihydrolipoyl dehydrogenase component (using dihydrolipoamide as a substrate) or in assays of bovine kidney pyruvate dehydrogenase complex. This indicates that the overall reaction catalyzed by the alpha-ketoglutarate dehydrogenase complex is required to elicit the effect of Ca2+ on NADH inhibition. At a fixed alpha-ketoglutarate concentration (50 microM), removal of Ca2+ reduced the activity of the alpha-ketoglutarate dehydrogenase complex by 8.5-fold (due to an increase in S0.5 for alpha-ketoglutarate) and, in the presence of different NADH:NAD+ ratios, decreased the activity of the complex by 50 to 100-fold. Effects of the phosphate potential (ATP/ADPxPi) or a combination of the phosphate potential and NADH:NAD+ ratio are also described. The possibility that the level of intramitochondrial free Ca/+ serves as a signal amplifier normally coupled to the energy state of mitochondria is discussed.

Overview of crew member energy expenditure during Shuttle Flight 61-8 EASE/ACCESS task performance

NASA Technical Reports Server (NTRS)

Horrigan, D. J.; Waligora, J. W.; Stanford, J.; Edwards, B. F.

1987-01-01

The energy expenditure of the Shuttle Flight 61-B crewmembers during the extravehicular performance of Experimental Assembly of Structures in EVA (EASE) and Assembly Concept of Construction of Space Structures (ACCESS) construction system tasks are reported. These data consist of metabolic rate time profiles correlated with specific EASE and ACCESS tasks and crew comments. Average extravehicular activity metabolic rates are computed and compared with those reported from previous Apollo, Shylab, and Shuttle flights. These data reflect total energy expenditure and not that of individual muscle groups such as hand and forearm. When correlated with specific EVA tasks and subtasks, the metabolic profile data is expected to be useful in planning future EVA protocols. For example, after experiencing high work rates and apparent overheating during some Gemini EVAs, it was found useful to carefully monitor work rates in subsequent flights to assess the adequacy of cooling garments and as an aid to preplanning EVA procedures. This presentation is represented by graphs and charts.

A fiber-optic sorbitol biosensor based on NADH fluorescence detection toward rapid diagnosis of diabetic complications.

PubMed

Gessei, Tomoko; Arakawa, Takahiro; Kudo, Hiroyuki; Mitsubayashi, Kohji

2015-09-21

Accumulation of sorbitol in the tissue is known to cause microvascular diabetic complications. In this paper, a fiber-optic biosensor for sorbitol which is used as a biomarker of diabetic complications was developed and tested. The biosensor used a sorbitol dehydrogenase from microorganisms of the genus Flavimonas with high substrate specificity and detected the fluorescence of reduced nicotinamide adenine dinucleotide (NADH) by the enzymatic reaction. An ultraviolet light emitting diode (UV-LED) was used as the excitation light source of NADH. The fluorescence of NADH was detected using a spectrometer or a photomultiplier tube (PMT). The UV-LED and the photodetector were coupled using a Y-shaped optical fiber. In the experiment, an optical fiber probe with a sorbitol dehydrogenase immobilized membrane was placed in a cuvette filled with a phosphate buffer containing the oxidized form of nicotinamide adenine dinucleotide (NAD(+)). The changes in NADH fluorescence intensity were measured after adding a standard sorbitol solution. According to the experimental assessment, the calibration range of the sorbitol biosensor systems using a spectrometer and a PMT was 5.0-1000 μmol L(-1) and 1.0-1000 μmol L(-1), respectively. The sorbitol biosensor system using the sorbitol dehydrogenase from microorganisms of the genus Flavimonas has high selectivity and sensitivity compared with that from sheep liver. The sorbitol biosensor allows for point-of-care testing applications or daily health care tests for diabetes patients.

Shuttle Upgrade Using 5-Segment Booster (FSB)

NASA Technical Reports Server (NTRS)

Sauvageau, Donald R.; Huppi, Hal D.; McCool, A. A. (Technical Monitor)

2000-01-01

In support of NASA's continuing effort to improve the over-all safety and reliability of the Shuttle system- a 5-segment booster (FSB) has been identified as an approach to satisfy that overall objective. To assess the feasibility of a 5-segment booster approach, NASA issued a feasibility study contract to evaluate the potential of a 5-segment booster to improve the overall capability of the Shuttle system, especially evaluating the potential to increase the system reliability and safety. In order to effectively evaluate the feasibility of the 5-segment concept, a four-member contractor team was established under the direction of NASA Marshall Space Flight Center (MSFC). MSFC provided the overall program oversight and integration as well as program contractual management. The contractor team consisted of Thiokol, Boeing North American Huntington Beach (BNA), Lockheed Martin Michoud Space Systems (LMMSS) and United Space Alliance (USA) and their subcontractor bd Systems (Control Dynamics Division, Huntsville, AL). United Space Alliance included the former members of United Space Booster Incorporated (USBI) who managed the booster element portion of the current Shuttle solid rocket boosters. Thiokol was responsible for the overall integration and coordination of the contractor team across all of the booster elements. They were also responsible for all of the motor modification evaluations. Boeing North American (BNA) was responsible for all systems integration analyses, generation of loads and environments. and performance and abort mode capabilities. Lockheed Martin Michoud Space Systems (LMMSS) was responsible for evaluating the impacts of any changes to the booster on the external tank (ET), and evaluating any design changes on the external tank necessary to accommodate the FSB. USA. including the former USBI contingent. was responsible for evaluating any modifications to facilities at the launch site as well as any booster component design modifications.

STS-82 Crew members pose in from of Discovery after Landing

NASA Technical Reports Server (NTRS)

1997-01-01

The STS-82 crew stands in front of the Space Shuttle Discovery after landing at KSC's Shuttle Landing Facility on Runway 15 to conclude a 10-day mission to service the orbiting Hubble Space Telescope (HST). Crew members are (from left to right) Mission Specialist Steven A. Hawley, Mission Commander Kenneth D. Bowersox, Mission Specialist Joseph R. 'Joe' Tanner, Pilot Scott J. 'Doc' Horowitz, Mission Specialist Gregory J. Harbaugh, Payload Commander Mark C. Lee and Mission Specialist Steven L. Smith. STS-82 is the ninth Shuttle nighttime landing, and the fourth nighttime landing at KSC. The seven-member crew performed a record-tying five back-to-back extravehicular activities (EVAs) or spacewalks to service the telescope, which has been in orbit for nearly seven years. Two new scientific instruments were installed, replacing two outdated instruments. Five spacewalks also were performed on the first servicing mission, STS-61, in December 1993. Only four spacewalks were scheduled for STS-82, but a fifth one was added during the flight to install several thermal blankets over some aging insulation covering three HST compartments containing key data processing, electronics and scientific instrument telemetry packages. STS-82 was the 82nd Space Shuttle flight and the second mission of 1997.

Space Shuttle Projects

NASA Image and Video Library

1994-02-25

This STS-68 patch was designed by artist Sean Collins. Exploration of Earth from space is the focus of the design of the insignia, the second flight of the Space Radar Laboratory (SRL-2). SRL-2 was part of NASA's Mission to Planet Earth (MTPE) project. The world's land masses and oceans dominate the center field, with the Space Shuttle Endeavour circling the globe. The SRL-2 letters span the width and breadth of planet Earth, symbolizing worldwide coverage of the two prime experiments of STS-68: The Shuttle Imaging Radar-C and X-Band Synthetic Aperture Radar (SIR-C/X-SAR) instruments; and the Measurement of Air Pollution from Satellites (MAPS) sensor. The red, blue, and black colors of the insignia represent the three operating wavelengths of SIR-C/X-SAR, and the gold band surrounding the globe symbolizes the atmospheric envelope examined by MAPS. The flags of international partners Germany and Italy are shown opposite Endeavour. The relationship of the Orbiter to Earth highlights the usefulness of human space flights in understanding Earth's environment, and the monitoring of its changing surface and atmosphere. In the words of the crew members, the soaring Orbiter also typifies the excellence of the NASA team in exploring our own world, using the tools which the Space Program developed to explore the other planets in the solar system.

Results of prototype software development for automation of shuttle proximity operations

NASA Technical Reports Server (NTRS)

Hiers, Harry K.; Olszewski, Oscar W.

1991-01-01

A Rendezvous Expert System (REX) was implemented on a Symbolics 3650 processor and integrated with the 6 DOF, high fidelity Systems Engineering Simulator (SES) at the NASA Johnson Space Center in Houston, Texas. The project goals were to automate the terminal phase of a shuttle rendezvous, normally flown manually by the crew, and proceed automatically to docking with the Space Station Freedom (SSF). The project goals were successfully demonstrated to various flight crew members, managers, and engineers in the technical community at JSC. The project was funded by NASA's Office of Space Flight, Advanced Program Development Division. Because of the complexity of the task, the REX development was divided into two distinct efforts. One to handle the guidance and control function using perfect navigation data, and another to provide the required visuals for the system management functions needed to give visibility to the crew members of the progress being made towards docking the shuttle with the LVLH stabilized SSF.

PAYLOAD (INDIA SATELLITE [INSAT]) - SHUTTLE

NASA Image and Video Library

1983-01-12

S83-36307 (2 June 1983) --- INSAT 1-B is being prepared for its trip aboard the space shuttle Challenger and its deployment for geosynchronous orbital duties at the Cape Canaveral Air Force Station and at NASA's Kennedy Space Center (KSC). The Indian National Satellite is the second such Indian communications/meteorological spacecraft, the first having been sent into space via a Delta launch vehicle. The STS-8 astronaut crew members and a payload assist module (PAM) will aid the newest INSAT in its deployment steps during NASA?s third Challenger flight in August of this year.

Inhibitors of type II NADH:menaquinone oxidoreductase represent a class of antitubercular drugs

PubMed Central

Weinstein, Edward A.; Yano, Takahiro; Li, Lin-Sheng; Avarbock, David; Avarbock, Andrew; Helm, Douglas; McColm, Andrew A.; Duncan, Ken; Lonsdale, John T.; Rubin, Harvey

2005-01-01

Mycobacterium tuberculosis (Mtb) is an obligate aerobe that is capable of long-term persistence under conditions of low oxygen tension. Analysis of the Mtb genome predicts the existence of a branched aerobic respiratory chain terminating in a cytochrome bd system and a cytochrome aa3 system. Both chains can be initiated with type II NADH:menaquinone oxidoreductase. We present a detailed biochemical characterization of the aerobic respiratory chains from Mtb and show that phenothiazine analogs specifically inhibit NADH:menaquinone oxidoreductase activity. The emergence of drug-resistant strains of Mtb has prompted a search for antimycobacterial agents. Several phenothiazines analogs are highly tuberculocidal in vitro, suppress Mtb growth in a mouse model of acute infection, and represent lead compounds that may give rise to a class of selective antibiotics. PMID:15767566

Shuttle/GPSPAC experimentation study

NASA Technical Reports Server (NTRS)

Moses, J.; Flack, J. F.

1977-01-01

The utilization is discussed of the GPSPAC, which is presently being developed to be used on the low altitude host vehicle (LAHV), for possible use in the shuttle avionics system to evaluate shuttle/GPS navigation performance. Analysis and tradeoffs of the shuttle/GPS link, shuttle signal interface requirements, oscillator tradeoffs and GPSPAC mechanical modifications for shuttle are included. Only the on-orbit utilization of GPSPAC for the shuttle is discussed. Other phases are briefly touched upon. Recommendations are provided for using the present GPSPAC and the changes required to perform shuttle on-orbit navigation.

STS-132 Space Shuttle Atlantis Launch

NASA Image and Video Library

2010-05-14

STS132-S-015 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

STS-132 Space Shuttle Atlantis Launch

NASA Image and Video Library

2010-05-14

STS132-S-016 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

STS-132 Space Shuttle Atlantis Launch

NASA Image and Video Library

2010-05-14

STS132-S-017 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

Effect of CO2 on NADH production of denitrifying microbes via inhibiting carbon source transport and its metabolism.

PubMed

Wan, Rui; Chen, Yinguang; Zheng, Xiong; Su, Yinglong; Huang, Haining

2018-06-15

The potential effect of CO 2 on environmental microbes has drawn much attention recently. As an important section of the nitrogen cycle, biological denitrification requires electron donor to reduce nitrogen oxide. Nicotinamide adenine dinucleotide (NADH), which is formed during carbon source metabolism, is a widely reported electron donor for denitrification. Here we studied the effect of CO 2 on NADH production and carbon source utilization in the denitrifying microbe Paracoccus denitrificans. We observed that NADH level was decreased by 45.5% with the increase of CO 2 concentration from 0 to 30,000ppm, which was attributed to the significantly decreased utilization of carbon source (i.e., acetate). Further study showed that CO 2 inhibited carbon source utilization because of multiple negative influences: (1) suppressing the growth and viability of denitrifier cells, (2) weakening the driving force for carbon source transport by decreasing bacterial membrane potential, and (3) downregulating the expression of genes encoding key enzymes involved in intracellular carbon metabolism, such as citrate synthase, aconitate hydratase, isocitrate dehydrogenase, succinate dehydrogenase, and fumarate reductase. This study suggests that the inhibitory effect of CO 2 on NADH production in denitrifiers might deteriorate the denitrification performance in an elevated CO 2 climate scenario. Copyright © 2018 Elsevier B.V. All rights reserved.

Development of a waste collection system for the space shuttle.

NASA Technical Reports Server (NTRS)

Behrend, A. F., Jr.; Swider, J. E., Jr.

1972-01-01

The development of a waste collection system to accommodate both male and female crew members for the space shuttle is discussed. The waste collection system, with emphasis on the collection and transfer of urine, is described. Human-interface requirements, zero-gravity influences and effects, and operational considerations required for total system design are discussed.

Renewable Molecular Flasks with NADH Models: Combination of Light-Driven Proton Reduction and Biomimetic Hydrogenation of Benzoxazinones.

PubMed

Zhao, Liang; Wei, Jianwei; Lu, Junhua; He, Cheng; Duan, Chunying

2017-07-17

Using small molecules with defined pockets to catalyze chemical transformations resulted in attractive catalytic syntheses that echo the remarkable properties of enzymes. By modulating the active site of a nicotinamide adenine dinucleotide (NADH) model in a redox-active molecular flask, we combined biomimetic hydrogenation with in situ regeneration of the active site in a one-pot transformation using light as a clean energy source. This molecular flask facilitates the encapsulation of benzoxazinones for biomimetic hydrogenation of the substrates within the inner space of the flask using the active sites of the NADH models. The redox-active metal centers provide an active hydrogen source by light-driven proton reduction outside the pocket, allowing the in situ regeneration of the NADH models under irradiation. This new synthetic platform, which offers control over the location of the redox events, provides a regenerating system that exhibits high selectivity and efficiency and is extendable to benzoxazinone and quinoxalinone systems. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Space Shuttle STS-87 Columbia launch

NASA Technical Reports Server (NTRS)

1997-01-01

Like a rising sun lighting up the afternoon sky, the Space Shuttle Columbia (STS-87) soared from Launch Pad 39B on the fourth flight of the United States Microgravity Payload (USMP-4) and Spartan-201 satellite which were managed by scientists and engineers from the Marshall Space Flight Center. During the 16-day mission, the crew oversaw experiments in microgravity; deployed and retrieved a solar satellite; and tested a new experimental camera, the AERCam Sprint. Two crew members, Dr. Takao Doi and Winston Scott also performed a spacewalk to practice International Space Station maneuvers.

FOOD - SHUTTLE

NASA Image and Video Library

1982-02-01

S82-26423 (January 1982) --- This is a close-up view of the rehydration unit to be used in meal preparation on operational space shuttle flights. The unit is located on the middeck of the space shuttles in the NASA fleet. Note the part of the food tray in upper right corner. Its six compartments (not all pictured) are used in space shuttle meal preparation and consumption. Photo credit: NASA

Shuttle-Mir: The United States and Russia Share History's Highest Stage

NASA Technical Reports Server (NTRS)

Morgan, Clay

2001-01-01

This book is an example of what may become the norm for history texts-an illustrated narrative accompanied by a CD-ROM. The text tells the story from the human side. It is based on reflections and quotes from the astronauts, cosmonauts, and team members who participated in the historic partnership. The main chapters give the perspectives of the seven U.S. astronauts living on Mir. The "STS boxes" share the Space Shuttle crews' experiences from the 11 Shuttle-Mir missions. The "Meanwhile on Earth" sections provide details of what was happening on Earth while the attention of the Program focused on the situations in space. The text reflects conventional usage; that is, temperature is given in degrees Fahrenheit, and metrics are used as appropriate. While this publication provides an accurate overview of the Shuttle-Mir Program, the reader is encouraged to explore the companion CD-ROM. It contains a complete, searchable text of the book itself plus source publications, mission data, status reports, drawings and illustrations, videos and hundreds of images, and even a virtual Shuttle-Mir children's book. Of particular interest are the Shuttle-Mir oral history transcripts and the letters written by American astronauts while they were in residence on the Russian space station. Many of the quotes in "Shuttle-Mir: The U.S. and Russia Share History's Highest Stage" came from sources located on the CD-ROM. The reader is invited to explore the Shuttle-Mir story through the words, images, and insights of those who took part in it.

In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox state.

PubMed

Zhao, Yuzheng; Wang, Aoxue; Zou, Yejun; Su, Ni; Loscalzo, Joseph; Yang, Yi

2016-08-01

NADH and its oxidized form NAD(+) have a central role in energy metabolism, and their concentrations are often considered to be among the most important readouts of metabolic state. Here, we present a detailed protocol to image and monitor NAD(+)/NADH redox state in living cells and in vivo using a highly responsive, genetically encoded fluorescent sensor known as SoNar (sensor of NAD(H) redox). The chimeric SoNar protein was initially developed by inserting circularly permuted yellow fluorescent protein (cpYFP) into the NADH-binding domain of Rex protein from Thermus aquaticus (T-Rex). It functions by binding to either NAD(+) or NADH, thus inducing protein conformational changes that affect its fluorescent properties. We first describe steps for how to establish SoNar-expressing cells, and then discuss how to use the system to quantify the intracellular redox state. This approach is sensitive, accurate, simple and able to report subtle perturbations of various pathways of energy metabolism in real time. We also detail the application of SoNar to high-throughput chemical screening of candidate compounds targeting cell metabolism in a microplate-reader-based assay, along with in vivo fluorescence imaging of tumor xenografts expressing SoNar in mice. Typically, the approximate time frame for fluorescence imaging of SoNar is 30 min for living cells and 60 min for living mice. For high-throughput chemical screening in a 384-well-plate assay, the whole procedure generally takes no longer than 60 min to assess the effects of 380 compounds on cell metabolism.

Space Shuttle Projects

NASA Image and Video Library

1992-05-11

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. Recorded with a 35 mm camera inside Endeavour’s cabin, is astronaut Pierre Thuot after his second unsuccessful attempt to affix a specially designed grapple bar to the 4.5 ton INTELSAT VI.

Animation graphic interface for the space shuttle onboard computer

NASA Technical Reports Server (NTRS)

Wike, Jeffrey; Griffith, Paul

1989-01-01

Graphics interfaces designed to operate on space qualified hardware challenge software designers to display complex information under processing power and physical size constraints. Under contract to Johnson Space Center, MICROEXPERT Systems is currently constructing an intelligent interface for the LASER DOCKING SENSOR (LDS) flight experiment. Part of this interface is a graphic animation display for Rendezvous and Proximity Operations. The displays have been designed in consultation with Shuttle astronauts. The displays show multiple views of a satellite relative to the shuttle, coupled with numeric attitude information. The graphics are generated using position data received by the Shuttle Payload and General Support Computer (PGSC) from the Laser Docking Sensor. Some of the design considerations include crew member preferences in graphic data representation, single versus multiple window displays, mission tailoring of graphic displays, realistic 3D images versus generic icon representations of real objects, the physical relationship of the observers to the graphic display, how numeric or textual information should interface with graphic data, in what frame of reference objects should be portrayed, recognizing conditions of display information-overload, and screen format and placement consistency.

Astronaut Nelson wipes off helmet visor in the middeck of Shuttle Challenger

NASA Technical Reports Server (NTRS)

1984-01-01

Astronaut George D. Nelson, 41-C mission specialist, wipes off his helmet visor in the middeck of Shuttle Challenger. Astronaut James D. van. Hoften, is seen in the background. Both crew members are wearing the liquid cooled undergarments for the extravehicular mobility unit (EMU).

Shuttle Safety Improvements

NASA Technical Reports Server (NTRS)

Henderson, Edward

2001-01-01

The Space Shuttle has been flying for over 20 years and based on the Orbiter design life of 100 missions it should be capable of flying at least 20 years more if we take care of it. The Space Shuttle Development Office established in 1997 has identified those upgrades needed to keep the Shuttle flying safely and efficiently until a new reusable launch vehicle (RLV) is available to meet the agency commitments and goals for human access to space. The upgrade requirements shown in figure 1 are to meet the program goals, support HEDS and next generation space transportation goals while protecting the country 's investment in the Space Shuttle. A major review of the shuttle hardware and processes was conducted in 1999 which identified key shuttle safety improvement priorities, as well as other system upgrades needed to reliably continue to support the shuttle miss ions well into the second decade of this century. The high priority safety upgrades selected for development and study will be addressed in this paper.

The Shuttle Era

NASA Technical Reports Server (NTRS)

1981-01-01

An overview of the Space Shuttle Program is presented. The missions of the space shuttle orbiters, the boosters and main engine, and experimental equipment are described. Crew and passenger accommodations are discussed as well as the shuttle management teams.

Characterization of the type 2 NADH:menaquinone oxidoreductases from Staphylococcus aureus and the bactericidal action of phenothiazines.

PubMed

Schurig-Briccio, Lici A; Yano, Takahiro; Rubin, Harvey; Gennis, Robert B

2014-07-01

Methicillin-resistant Staphylococcus aureus (MRSA) is currently one of the principal multiple drug resistant bacterial pathogens causing serious infections, many of which are life-threatening. Consequently, new therapeutic targets are required to combat such infections. In the current work, we explore the type 2 Nicotinamide adenine dinucleotide reduced form (NADH) dehydrogenases (NDH-2s) as possible drug targets and look at the effects of phenothiazines, known to inhibit NDH-2 from Mycobacterium tuberculosis. NDH-2s are monotopic membrane proteins that catalyze the transfer of electrons from NADH via flavin adenine dinucleotide (FAD) to the quinone pool. They are required for maintaining the NADH/Nicotinamide adenine dinucleotide (NAD(+)) redox balance and contribute indirectly to the generation of proton motive force. NDH-2s are not present in mammals, but are the only form of respiratory NADH dehydrogenase in several pathogens, including S. aureus. In this work, the two putative ndh genes present in the S. aureus genome were identified, cloned and expressed, and the proteins were purified and characterized. Phenothiazines were shown to inhibit both of the S. aureus NDH-2s with half maximal inhibitory concentration (IC50) values as low as 8μM. However, evaluating the effects of phenothiazines on whole cells of S. aureus was complicated by the fact that they are also acting as uncouplers of oxidative phosphorylation. This article is part of a Special Issue entitled: 18th European Bioenergetic Conference. Copyright © 2014 Elsevier B.V. All rights reserved.

Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients

NASA Technical Reports Server (NTRS)

Cho, NaMi; Chueh, Pin-Ju; Kim, Chinpal; Caldwell, Sara; Morre, Dorothy M.; Morre, D. James

2002-01-01

Monoclonal antibodies were generated in mice to a 34-kDa circulating form of a drug-responsive hydroquinone (NADH) oxidase with a protein disulfide-thiol interchange activity specific to the surface of cancer cells and the sera of cancer patients. Screening used Western blots with purified 34-kDa tNOX from HeLa cells and the sera of cancer patients. Epitopes were sought that inhibited the drug-responsive oxidation of NADH with the sera of cancer patients, but which had no effect on NADH oxidation with the sera of healthy volunteers. Two such antisera were generated. One, designated monoclonal antibody (mAb) 12.1, was characterized extensively. The NADH oxidase activity inhibited by mAb 12.1 also was inhibited by the quinone site inhibitor capsaicin (8-methyl- N-vanillyl-6-noneamide). The inhibition was competitive for the drug-responsive protein disulfide-thiol interchange activity assayed either by restoration of activity to scrambled RNase or by cleavage of a dithiodipyridine substrate, and was uncompetitive for NADH oxidation. Both the mAb 12.1 and the postimmune antisera immunoprecipitated drug-responsive NOX activity and identified the same 34-kDa tNOX protein in the sera of cancer patients that was absent from sera of healthy volunteers, and was utilized as immunogen. Preimmune sera from the same mouse as the postimmune antisera was without effect. Both mouse ascites containing mAb 12.1 and postimmune sera (but not preimmune sera) slowed the growth of human cancer cell lines in culture, but did not affect the growth of non-cancerous cell lines. Immunocytochemical and histochemical findings showed that mAb 12.1 reacted with the surface membranes of human carcinoma cells and tissues.

Shuttle Carrier Aircraft

NASA Image and Video Library

2014-04-23

It has been called the world's greatest piggyback ride: a space shuttle, atop a Boeing 747 jet aircraft. But this is no ordinary 747, this is the Shuttle Carrier Aircraft...the SCA. This specially modified jumbo jet was not only a taxi service for the shuttle, but also helped in the development of the shuttle itself. In 30 years of flying, the majestic image of a spacecraft joined to the SCA, became a symbol of American invention and ingenuity.

Autonomous Space Shuttle

NASA Technical Reports Server (NTRS)

Siders, Jeffrey A.; Smith, Robert H.

2004-01-01

The continued assembly and operation of the International Space Station (ISS) is the cornerstone within NASA's overall Strategic P an. As indicated in NASA's Integrated Space Transportation Plan (ISTP), the International Space Station requires Shuttle to fly through at least the middle of the next decade to complete assembly of the Station, provide crew transport, and to provide heavy lift up and down mass capability. The ISTP reflects a tight coupling among the Station, Shuttle, and OSP programs to support our Nation's space goal . While the Shuttle is a critical component of this ISTP, there is a new emphasis for the need to achieve greater efficiency and safety in transporting crews to and from the Space Station. This need is being addressed through the Orbital Space Plane (OSP) Program. However, the OSP is being designed to "complement" the Shuttle as the primary means for crew transfer, and will not replace all the Shuttle's capabilities. The unique heavy lift capabilities of the Space Shuttle is essential for both ISS, as well as other potential missions extending beyond low Earth orbit. One concept under discussion to better fulfill this role of a heavy lift carrier, is the transformation of the Shuttle to an "un-piloted" autonomous system. This concept would eliminate the loss of crew risk, while providing a substantial increase in payload to orbit capability. Using the guidelines reflected in the NASA ISTP, the autonomous Shuttle a simplified concept of operations can be described as; "a re-supply of cargo to the ISS through the use of an un-piloted Shuttle vehicle from launch through landing". Although this is the primary mission profile, the other major consideration in developing an autonomous Shuttle is maintaining a crew transportation capability to ISS as an assured human access to space capability.

Intracellular Redox State Revealed by In Vivo 31P MRS Measurement of NAD+ and NADH Contents in Brains

PubMed Central

Lu, Ming; Zhu, Xiao-Hong; Zhang, Yi; Chen, Wei

2015-01-01

Purpose Nicotinamide adenine dinucleotide (NAD), in oxidized (NAD+) or reduced (NADH) form, plays key roles in cellular metabolism. Intracellular NAD+/NADH ratio represents the cellular redox state; however, it is difficult to measure in vivo. We report here a novel in vivo 31P MRS method for noninvasive measurement of intracellular NAD concentrations and NAD+/NADH ratio in the brain. Methods It uses a theoretical model to describe the NAD spectral patterns at a given field for quantification. Standard NAD solutions and independent cat brain measurements at 9.4 T and 16.4 T were used to evaluate this method. We also measured T1 values of brain NAD. Results Model simulation and studies of solutions and brains indicate that the proposed method can quantify submillimolar NAD concentrations with reasonable accuracy if adequate 31P MRS signal-to-noise ratio and linewidth were obtained. The NAD concentrations and NAD+/NADH ratio of cat brains measured at 16.4 T and 9.4 T were consistent despite the significantly different T1 values and NAD spectra patterns at two fields. Conclusion This newly established 31P MRS method makes it possible for the first time to noninvasively study the intracellular redox state and its roles in brain functions and diseases, and it can potentially be applied to other organs. PMID:23843330

Space Shuttle Projects

NASA Image and Video Library

2001-01-01

The Space Shuttle represented an entirely new generation of space vehicles, the world's first reusable spacecraft. Unlike earlier expendable rockets, the Shuttle was designed to be launched over and over again and would serve as a system for ferrying payloads and persornel to and from Earth orbit. The Shuttle's major components are the orbiter spacecraft; the three main engines, with a combined thrust of more than 1.2 million pounds; the huge external tank (ET) that feeds the liquid hydrogen fuel and liquid oxygen oxidizer to the three main engines; and the two solid rocket boosters (SRB's), with their combined thrust of some 5.8 million pounds, that provide most of the power for the first two minutes of flight. Crucially involved with the Space Shuttle program virtually from its inception, the Marshall Space Flight Center (MSFC) played a leading role in the design, development, testing, and fabrication of many major Shuttle propulsion components. The MSFC was assigned responsibility for developing the Shuttle orbiter's high-performance main engines, the most complex rocket engines ever built. The MSFC was also responsible for developing the Shuttle's massive ET and the solid rocket motors and boosters.

Space Shuttle Projects

NASA Image and Video Library

1975-01-01

The Space Shuttle represented an entirely new generation of space vehicle, the world's first reusable spacecraft. Unlike earlier expendable rockets, the Shuttle was designed to be launched over and over again and would serve as a system for ferrying payloads and persornel to and from Earth orbit. The Shuttle's major components are the orbiter spacecraft; the three main engines, with a combined thrust of more than 1.2 million pounds; the huge external tank (ET) that feeds the liquid hydrogen fuel and liquid oxygen oxidizer to the three main engines; and the two solid rocket boosters (SRB's), with their combined thrust of some 5.8 million pounds. The SRB's provide most of the power for the first two minutes of flight. Crucially involved with the Space Shuttle program virtually from its inception, the Marshall Space Flight Center (MSFC) played a leading role in the design, development, testing, and fabrication of many major Shuttle propulsion components. The MSFC was assigned responsibility for developing the Shuttle orbiter's high-performance main engines, the most complex rocket engines ever built. The MSFC was also responsible for developing the Shuttle's massive ET and the solid rocket motors and boosters.

Cooling Properties of the Shuttle Advanced Crew Escape Spacesuit: Results of an Environmental Chamber Experiment

NASA Technical Reports Server (NTRS)

Hamilton, Douglas; Gillis, David; Bue, Grant; Son, Chan; Norcross, Jason; Kuznetz, Larry; Chapman, Kirt; Chhipwadia, Ketan; McBride, Tim

2008-01-01

The shuttle crew wears the Advanced Crew Escape Spacesuit (ACES) to protect themselves from cabin decompression and to support bail out during landing. ACES is cooled by a liquid-cooled garment (LCG) that interfaces to a heat exchanger that dumps heat into the cabin. The ACES outer layer is made of Gore-Tex(Registered TradeMark), permitting water vapor to escape while containing oxygen. The crew can only lose heat via insensible water losses and the LCG. Under nominal landing operations, the average cabin temperature rarely exceeds 75 F, which is adequate for the ACES to function. Problem A rescue shuttle will need to return 11 crew members if the previous mission suffers a thermal protection system failure, preventing it from returning safely to Earth. Initial analysis revealed that 11 crew members in the shuttle will increase cabin temperature at wheel stop above 80 F, which decreases the ACES ability to keep crew members cool. Air flow in the middeck of the shuttle is inhomogeneous and some ACES may experience much higher temperatures that could cause excessive thermal stress to crew members. Methods A ground study was conducted to measure the cooling efficiency of the ACES at 75 F, 85 F, and 95 F at 50% relative humidity. Test subjects representing 5, 50, and 95 percentile body habitus of the astronaut corps performed hand ergometry keeping their metabolic rate at 400, 600, and 800 BTU/hr for one hour. Core temperature was measured by rectal probe and skin, while inside and outside the suit. Environmental chamber wall and cooling unit inlet and outlet temperatures were measured using high-resolution thermistors ( 0.2 C). Conclusions Under these test conditions, the ACES was able to protect the core temperature of all test subjects, however thermal stress due to high insensible losses and skin temperature and skin heat flow may impact crew performance. Further research should be performed to understand the impact on cognitive performance.

Improvement of exopolysaccharide production in Lactobacillus casei LC2W by overexpression of NADH oxidase gene.

PubMed

Li, Nan; Wang, Yuanlong; Zhu, Ping; Liu, Zhenmin; Guo, Benheng; Ren, Jing

2015-02-01

Lactobacillus casei LC2W is an exopolysaccharide (EPS)-producing strain with probiotic effects. To investigate the regulation mechanism of EPS biosynthesis and to improve EPS production through cofactor engineering, a H₂O-forming NADH oxidase gene was cloned from Streptococcus mutans and overexpressed in L. casei LC2W under the control of constitutive promoter P₂₃. The recombinant strain LC-nox exhibited 0.854 U/mL of NADH oxidase activity, which was elevated by almost 20-fold in comparison with that of wild-type strain. As a result, overexpression of NADH oxidase resulted in a reduction in growth rate. In addition, lactate production was decreased by 22% in recombinant strain. It was proposed that more carbon source was saved and used for the biosynthesis of EPS, the production of which was reached at 219.4 mg/L, increased by 46% compared to that of wild-type strain. This work provided a novel and convenient genetic approach to manipulate metabolic flux and to increase EPS production. To the best of our knowledge, this is the first report which correlates cofactor engineering with EPS production. Copyright © 2015 Elsevier GmbH. All rights reserved.

Determination of the in vivo NAD:NADH ratio in Saccharomyces cerevisiae under anaerobic conditions, using alcohol dehydrogenase as sensor reaction.

PubMed

Bekers, K M; Heijnen, J J; van Gulik, W M

2015-08-01

With the current quantitative metabolomics techniques, only whole-cell concentrations of NAD and NADH can be quantified. These measurements cannot provide information on the in vivo redox state of the cells, which is determined by the ratio of the free forms only. In this work we quantified free NAD:NADH ratios in yeast under anaerobic conditions, using alcohol dehydrogenase (ADH) and the lumped reaction of glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase as sensor reactions. We showed that, with an alternative accurate acetaldehyde determination method, based on rapid sampling, instantaneous derivatization with 2,4 diaminophenol hydrazine (DNPH) and quantification with HPLC, the ADH-catalysed oxidation of ethanol to acetaldehyde can be applied as a relatively fast and simple sensor reaction to quantify the free NAD:NADH ratio under anaerobic conditions. We evaluated the applicability of ADH as a sensor reaction in the yeast Saccharomyces cerevisiae, grown in anaerobic glucose-limited chemostats under steady-state and dynamic conditions. The results found in this study showed that the cytosolic redox status (NAD:NADH ratio) of yeast is at least one order of magnitude lower, and is thus much more reduced, under anaerobic conditions compared to aerobic glucose-limited steady-state conditions. The more reduced state of the cytosol under anaerobic conditions has major implications for (central) metabolism. Accurate determination of the free NAD:NADH ratio is therefore of importance for the unravelling of in vivo enzyme kinetics and to judge accurately the thermodynamic reversibility of each redox reaction. Copyright © 2015 John Wiley & Sons, Ltd.

Space Shuttle Projects

NASA Image and Video Library

1992-05-07

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. The 4.5 ton INTELSAT VI was successfully snared by three astronauts on a third EVA. The three hand-grabbed the errant satellite, pulled it into the cargo bay, and attached a boost-given perigee stage before its release.

Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth

DOE PAGES

Beckwith, Christopher R.; Edwards, Marcus J.; Lawes, Matthew; ...

2015-04-28

The autotrophic Sideroxydans lithotrophicus ES-1 can grow by coupling the oxidation of ferrous iron to the reduction of oxygen. Soluble ferrous iron is oxidized at the surface of the cell by an MtoAB porin-cytochrome complex that functions as an electron conduit through the outer membrane. Electrons are then transported to the cytoplasmic membrane where they are used to generate proton motive force (PMF) (for ATP synthesis) and NADH for autotrophic processes such as carbon fixation. As part of the mtoAB gene cluster, S. lithotrophicus also contains the gene mtoD that is proposed to encode a cytochrome c protein. We isolatedmore » mtoD from a Shewanella oneidensis expression system where the mtoD gene was expressed on a pBAD plasmid vector. Biochemical, biophysical, and crystallographic characterization of the purified MtoD revealed it as an 11 kDa monomeric protein containing a single heme. Sequence and structural alignment indicated that MtoD belonged to the class-1 cytochrome c family and had a similar fold to ferricytochrome c552 family, however the MtoD heme is bis-histidine coordinated and is substantially more exposed than the hemes of other family members. The reduction potential of the MtoD heme at pH 7 was +155 mV vs. Standard Hydrogen Electrode, which is approximately 100 mV lower than that of mitochondrial cytochrome c. Consideration of the properties of MtoD in the context of the potential respiratory partners identified from the genome suggests that MtoD could associate to multiple electron transfer partners as the primary periplasmic electron shuttle.« less

NASA Space Shuttle Program: Shuttle Environmental Assurance (SEA) Initiative

NASA Technical Reports Server (NTRS)

Glover, Steve E.; McCool, Alex (Technical Monitor)

2002-01-01

The first Space Shuttle flight was in 1981 and the fleet was originally expected to be replaced with a new generation vehicle in the early 21st century. Space Shuttle Program (SSP) elements proactively address environmental and obsolescence concerns and continue to improve safety and supportability. The SSP manager created the Shuttle Environmental Assurance (SEA) Initiative in 2000. SEA is to provide an integrated approach for the SSP to promote environmental excellence, proactively manage materials obsolescence, and optimize associated resources.

Space shuttle requirements/configuration evolution

NASA Technical Reports Server (NTRS)

Andrews, E. P.

1991-01-01

Space Shuttle chronology; Space Shuttle comparison; Cost comparison; Performance; Program ground rules; Sizing criteria; Crew/passenger provisions; Space Shuttle Main Engine (SSME) characteristics; Space Shuttle program milestones; and Space Shuttle requirements are outlined. This presentation is represented by viewgraphs.

Characterization of Truncated Tumor-Associated NADH Oxidase (ttNOX)

NASA Technical Reports Server (NTRS)

Karr, Laurel J.; Malone, Christine C.; Burk, Melissa; Moore, Blake P.; Achari, Aniruddha; Curreri, Peter A. (Technical Monitor)

2002-01-01

Bacterial, plant and animal cells possess novel surface proteins that exhibit both NADH oxidation (NOX) or hydroquinone and protein disulfide-thiol interchange. These enzymatic activities alternate to yield oscillating patterns wjth period lengths of approximately 24 minutes. The catalytic period of NOX proteins are temperature compensated and gravity responsive. We report the cloning, expression and characterization of truncated tumor-associated NADH oxidase (ttNOX), in which the membrane spanning region has been deleted. The cDNA (originated from HeLa cells) was cloned into pET-34b and pET-14b (Novagen) vectors for E. coli expression. Optimized expression and purification protocols yielded greater than 300mg per liter of culture with greater than 95% purity. Circular dichroism data was collected from a 2.7mg/ml solution in a 0.1mm cuvette with variable scanning using an Olis RSM CD spectrophotometer. The ellipticity values were scanned from 190 to 260nm. The spectra recorded have characteristics for alpha proteins with band maxima at 216nm and a possible shoulder at 212nm at 12OC and 250 C. Protein crystal screens are in progress and, to date, only small crystals have been observed. The regular periodic oscillatory change in the ttNOX protein is indicative of a possible time-keeping functional role. A single protein possessing alternating catalytic activities, with a potential biological clock function, is unprecedented and structural determination is paramount to understanding this role.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-035 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-051 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-053 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-061 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-036 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-060 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-039 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-040 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-056 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-044 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-063 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-062 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-050 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-064 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-058 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-052 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-038 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-042 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-055 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-065 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-037 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-057 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-059 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-033 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell..

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-066 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-054 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-067 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-047 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-030 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-048 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-045 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-041 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-049 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-043 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-068 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-034 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-069 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Rusty Backer and Michael Gayle

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-046 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Tony Gray and Tom Farrar

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-031 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

STS-84 / Mir 23 Crew Member Jerry Linenger post landing

NASA Technical Reports Server (NTRS)

1997-01-01

KENNEDY SPACE CENTER, FL - Astronaut and recent Mir 23 crew member Jerry M. Linenger holds a stuffed bear he was given by NASA Administrator Daniel Goldin at the conclusion of the STS-84 Space Shuttle mission. STS-84 was the sixth docking of the Shuttle with the Russian Space Station Mir, where Linenger has lived and worked the past four months. Goldin presented several gifts to Linenger in the Crew Transport Vehicle shortly after landing of the Space Shuttle orbiter Atlantis on KSC's Runway 33. Besides the bear for Linenger's 18-month-old son, John, Goldin gave the astronaut flowers for Linenger's wife, Kathryn; and a rattle for the Linengers' unborn child who is due next month. Linenger was replaced on the Russian space station by STS-84 Mission Specialist C. Michael Foale.

The NADH:flavin oxidoreductase Nox from Rhodococcus erythropolis MI2 is the key enzyme of 4,4'-dithiodibutyric acid degradation.

PubMed

Khairy, H; Wübbeler, J H; Steinbüchel, A

2016-12-01

The reduction of the disulphide bond is the initial catabolic step of the microbial degradation of the organic disulphide 4,4'-dithiodibutyric acid (DTDB). Previously, an NADH:flavin oxidoreductase from Rhodococcus erythropolis MI2 designated as Nox MI2 , which belongs to the old yellow enzyme (OYE) family, was identified. In the present study, it was proven that Nox MI2 has the ability to cleave the sulphur-sulphur bond in DTDB. In silico analysis revealed high sequence similarities to proteins of the flavin mononucleotide (FMN) reductase family identified in many strains of R. erythropolis. Therefore, nox was heterologously expressed in the pET23a(+) expression system using Escherichia coli strain BL21(DE3) pLysS, which effectively produces soluble active Nox MI2 . Nox MI2 showed a maximum specific activity (V max ) of 3·36 μmol min -1  mg -1 corresponding to a k cat of 2·5 s -1 and an apparent substrate K m of 0·6 mmol l -1 , when different DTDB concentrations were applied. No metal cofactors were required. Moreover, Nox MI2 had very low activity with other sulphur-containing compounds like 3,3'-dithiodipropionic acid (8·0%), 3,3'-thiodipropionic acid (7·6%) and 5,5'-dithiobis(2-nitrobenzoic acid) (8·0%). The UV/VIS spectrum of Nox MI2 revealed the presence of the cofactor FMN. Based on results obtained, Nox MI2 adds a new physiological substrate and mode of action to OYE members. It was unequivocally demonstrated in this study that an NADH:flavin oxidoreductase from Rhodococcus erythropolis MI2 (Nox MI2 ) is able to cleave the xenobiotic disulphide 4,4'-dithiodibutyric acid (DTDB) into two molecules of 4-mercaptobutyric acid (4MB) with concomitant consumption of NADH. Nox MI2 showed a high substrate specificity as well as high heat stability. This study provides the first detailed characterization of the initial cleavage of DTDB, which is considered as a promising polythioester precursor. © 2016 The Society for Applied Microbiology.

Space Shuttle Columbia touches down on Runway 33

NASA Technical Reports Server (NTRS)

1997-01-01

KENNEDY SPACE CENTER, FLA. -- The Space Shuttle Columbia touches down on Runway 33 at KSC''';s Shuttle Landing Facility at 2:33:11 p.m. EDT, April 8, to conclude the Microgravity Science Laboratory-1 (MSL-1) mission. At main gear touchdown, the STS-83 mission duration was 3 days, 23 hours, 12 minutes. The planned 16-day mission was cut short by a faulty fuel cell. This is only the third time in Shuttle program history that an orbiter was brought home early due to mechanical problems. This was also the 36th KSC landing since the program began in 1981. Mission Commander James D. Halsell, Jr. flew Columbia to a perfect landing with help from Pilot Susan L. Still. Other crew members are Payload Commander Janice E. Voss; Mission Specialists Michael L. Gernhardt and Donald A. Thomas; and Payload Specialists Roger K. Crouch and Gregory T. Linteris. In spite of the abbreviated flight, the crew was able to perform MSL-1 experiments. The Spacelab-module-based experiments were used to test some of the hardware, facilities and procedures that are planned for use on the International Space Station and to conduct combustion, protein crystal growth and materials processing investigations.

STS-84 Shuttle Atlantis Liftoff

NASA Technical Reports Server (NTRS)

1997-01-01

The Space Shuttle Atlantis turns night into day for a few moments as it lifts off on May 15 at 4:07:48 a.m. EDT from Launch Pad 39A on the STS-84 mission. The fourth Shuttle mission of 1997 will be the sixth docking of the Space Shuttle with the Russian Space Station Mir. The commander is Charles J. Precourt. The pilot is Eileen Marie Collins. The five mission specialists are C. Michael Foale, Carlos I. Noriega, Edward Tsang Lu, Jean-Francois Clervoy of the European Space Agency and Elena V. Kondakova of the Russian Space Agency. The planned nine-day mission will include the exchange of Foale for U.S. astronaut and Mir 23 crew member Jerry M. Linenger, who has been on Mir since Jan. 15. Linenger transferred to Mir during the last docking mission, STS-81; he will return to Earth on Atlantis. Foale is slated to remain on Mir for about four months until he is replaced in September by STS-86 Mission Specialist Wendy B. Lawrence. During the five days Atlantis is scheduled to be docked with the Mir, the STS-84 crew and the Mir 23 crew, including two Russian cosmonauts, Commander Vasily Tsibliev and Flight Engineer Alexander Lazutkin, will participate in joint experiments. The STS-84 mission also will involve the transfer of more than 7,300 pounds of water, logistics and science equipment to and from the Mir. Atlantis is carrying a nearly 300-pound oxygen generator to replace one of two Mir units which have experienced malfunctions. The oxygen it generates is used for breathing by the Mir crew.

Shuttle Topography Data Inform Solar Power Analysis

NASA Technical Reports Server (NTRS)

2013-01-01

The next time you flip on a light switch, there s a chance that you could be benefitting from data originally acquired during the Space Shuttle Program. An effort spearheaded by Jet Propulsion Laboratory (JPL) and the National Geospatial-Intelligence Agency (NGA) in 2000 put together the first near-global elevation map of the Earth ever assembled, which has found use in everything from 3D terrain maps to models that inform solar power production. For the project, called the Shuttle Radar Topography Mission (SRTM), engineers at JPL designed a 60-meter mast that was fitted onto Shuttle Endeavour. Once deployed in space, an antenna attached to the end of the mast worked in combination with another antenna on the shuttle to simultaneously collect data from two perspectives. Just as having two eyes makes depth perception possible, the SRTM data sets could be combined to form an accurate picture of the Earth s surface elevations, the first hight-detail, near-global elevation map ever assembled. What made SRTM unique was not just its surface mapping capabilities but the completeness of the data it acquired. Over the course of 11 days, the shuttle orbited the Earth nearly 180 times, covering everything between the 60deg north and 54deg south latitudes, or roughly 80 percent of the world s total landmass. Of that targeted land area, 95 percent was mapped at least twice, and 24 percent was mapped at least four times. Following several years of processing, NASA released the data to the public in partnership with NGA. Robert Crippen, a member of the SRTM science team, says that the data have proven useful in a variety of fields. "Satellites have produced vast amounts of remote sensing data, which over the years have been mostly two-dimensional. But the Earth s surface is three-dimensional. Detailed topographic data give us the means to visualize and analyze remote sensing data in their natural three-dimensional structure, facilitating a greater understanding of the features

ITOS/space shuttle study

NASA Technical Reports Server (NTRS)

1971-01-01

The results are reported of a study to explore the potential cost reductions in the operational ITOS weather satellite program as a consequence of shuttle/bug availability for satellite placement and retrieval, and satellite servicing and maintenance. The study program was divided into shuttle impact on equipment and testing costs, and shuttle impact on overall future ITOS operational program costs, and shuttle impact on configuration. It is concluded that savings in recurring spacecraft costs can be realized in the 1978 ITOS program, if a space shuttle is utilized.

Shuttle plate braiding machine

NASA Technical Reports Server (NTRS)

Huey, Jr., Cecil O. (Inventor)

1994-01-01

A method and apparatus for moving yarn in a selected pattern to form a braided article. The apparatus includes a segmented grid of stationary support elements and a plurality of shuttles configured to carry yarn. The shuttles are supported for movement on the grid assembly and each shuttle includes a retractable plunger for engaging a reciprocating shuttle plate that moves below the grid assembly. Such engagement at selected times causes the shuttles to move about the grid assembly in a selected pattern to form a braided article of a particular geometry.

NADH-ubiquinone oxidoreductase activity in the kinetoplasts of the plant trypanosomatid Phytomonas serpens.

PubMed

González-Halphen, Diego; Maslov, Dmitri A

2004-03-01

NADH-ubiquinone oxidoreductase activity is present in mitochondrial lysates of Phytomonas serpens. Rotenone at 2-10 microM inhibited the activity 50-75%, indicating that it belongs to respiratory complex I. The activity was also inhibited 50-60% in the presence of 10-30 nM atovaquone suggesting that inhibition of complex I represents a likely mechanism of the known antileishmanial activity of this drug. The complex was partially purified by chromatography on DEAE-Sepharose CL-6B and gel-filtration on Sepharose CL-2B. The NADH:ubiquinone oxidoreductase activity in this preparation was completely inactivated by 20 nM atovaquone. The partially purified complex was present in a low amount and its subunits could not be discerned by staining with Coomassie. However, one of its components, a homologue of the 39 kDa subunit of the bovine complex I, was identified immunochemically in the original lysate and in the partially purified material.

Disruption of key NADH-binding pocket residues of the Mycobacterium tuberculosis InhA affects DD-CoA binding ability.

PubMed

Shaw, Daniel J; Robb, Kirsty; Vetter, Beatrice V; Tong, Madeline; Molle, Virginie; Hunt, Neil T; Hoskisson, Paul A

2017-07-05

Tuberculosis (TB) is a global health problem that affects over 10 million people. There is an urgent need to develop novel antimicrobial therapies to combat TB. To achieve this, a thorough understanding of key validated drug targets is required. The enoyl reductase InhA, responsible for synthesis of essential mycolic acids in the mycobacterial cell wall, is the target for the frontline anti-TB drug isoniazid. To better understand the activity of this protein a series of mutants, targeted to the NADH co-factor binding pocket were created. Residues P193 and W222 comprise a series of hydrophobic residues surrounding the cofactor binding site and mutation of both residues negatively affect InhA function. Construction of an M155A mutant of InhA results in increased affinity for NADH and DD-CoA turnover but with a reduction in V max for DD-CoA, impairing overall activity. This suggests that NADH-binding geometry of InhA likely permits long-range interactions between residues in the NADH-binding pocket to facilitate substrate turnover in the DD-CoA binding region of the protein. Understanding the precise details of substrate binding and turnover in InhA and how this may affect protein-protein interactions may facilitate the development of improved inhibitors enabling the development of novel anti-TB drugs.

2,4-Dichlorophenoxyacetic Acid Inhibits the Outer Membrane NADH Dehydrogenase of Plant Mitochondria 1

PubMed Central

Mannella, Carmen A.; Bonner, Walter D.

1978-01-01

The NADH dehydrogenase of potato (Solanum tuberosum) and mung bean (Phaseolus aureus) outer mitochondrial membranes is specifically inhibited by both 2,4-dichlorophenoxyacetic and 2,4,5-trichlorophenoxyacetic acids but not by the natural auxin indole-3-acetic acid. PMID:16660539

Reduction of Clofazimine by Mycobacterial Type 2 NADH:Quinone Oxidoreductase

PubMed Central

Yano, Takahiro; Kassovska-Bratinova, Sacha; Teh, J. Shin; Winkler, Jeffrey; Sullivan, Kevin; Isaacs, Andre; Schechter, Norman M.; Rubin, Harvey

2011-01-01

The mechanism of action of clofazimine (CFZ), an antimycobacterial drug with a long history, is not well understood. The present study describes a redox cycling pathway that involves the enzymatic reduction of CFZ by NDH-2, the primary respiratory chain NADH:quinone oxidoreductase of mycobacteria and nonenzymatic oxidation of reduced CFZ by O2 yielding CFZ and reactive oxygen species (ROS). This pathway was demonstrated using isolated membranes and purified recombinant NDH-2. The reduction and oxidation of CFZ was measured spectrally, and the production of ROS was measured using a coupled assay system with Amplex Red. Supporting the ROS-based killing mechanism, bacteria grown in the presence of antioxidants are more resistant to CFZ. CFZ-mediated increase in NADH oxidation and ROS production were not observed in membranes from three different Gram-negative bacteria but was observed in Staphylococcus aureus and Saccharomyces cerevisiae, which is consistent with the known antimicrobial specificity of CFZ. A more soluble analog of CFZ, KS6, was synthesized and was shown to have the same activities as CFZ. These studies describe a pathway for a continuous and high rate of reactive oxygen species production in Mycobacterium smegmatis treated with CFZ and a CFZ analog as well as evidence that cell death produced by these agents are related to the production of these radical species. PMID:21193400

Photo of family members of STS-5 commander, Vance D. Brand

NASA Technical Reports Server (NTRS)

1982-01-01

Erik Brand and his mother Beverly are seen in a photo of family members of STS-5 commander Vance D. Brand. Erik holds a small model of the space shuttle with its solid rocket boosters and external fuel tank still attached.

The ins and outs of FoxO shuttling: mechanisms of FoxO translocation and transcriptional regulation.

PubMed Central

Van Der Heide, Lars P; Hoekman, Marco F M; Smidt, Marten P

2004-01-01

FoxO (forkhead box O; forkhead members of the O class) are transcription factors that function under the control of insulin/insulin-like signalling. FoxO factors have been associated with a multitude of biological processes, including cell-cycle, cell death, DNA repair, metabolism and protection from oxidative stress. Central to the regulation of FoxO factors is a shuttling system, which confines FoxO factors to either the nucleus or the cytosol. Shuttling of FoxO requires protein phosphorylation within several domains, and association with 14-3-3 proteins and the nuclear transport machinery. Description of the FoxO-shuttling mechanism contributes to the understanding of FoxO function in relation to signalling and gene regulation. PMID:15005655

Structural and Functional insights into the catalytic mechanism of the Type II NADH:quinone oxidoreductase family

PubMed Central

Marreiros, Bruno C.; Sena, Filipa V.; Sousa, Filipe M.; Oliveira, A. Sofia F.; Soares, Cláudio M.; Batista, Ana P.; Pereira, Manuela M.

2017-01-01

Type II NADH:quinone oxidoreductases (NDH-2s) are membrane proteins involved in respiratory chains. These proteins contribute indirectly to the establishment of the transmembrane difference of electrochemical potential by catalyzing the reduction of quinone by oxidation of NAD(P)H. NDH-2s are widespread enzymes being present in the three domains of life. In this work, we explored the catalytic mechanism of NDH-2 by investigating the common elements of all NDH-2s, based on the rationale that conservation of such elements reflects their structural/functional importance. We observed conserved sequence motifs and structural elements among 1762 NDH-2s. We identified two proton pathways possibly involved in the protonation of the quinone. Our results led us to propose the first catalytic mechanism for NDH-2 family, in which a conserved glutamate residue, E172 (in NDH-2 from Staphylococcus aureus) plays a key role in proton transfer to the quinone pocket. This catalytic mechanism may also be extended to the other members of the two-Dinucleotide Binding Domains Flavoprotein (tDBDF) superfamily, such as sulfide:quinone oxidoreductases. PMID:28181562

STS-77 Shuttle Endeavour clears tower (front left)

NASA Technical Reports Server (NTRS)

1996-01-01

A flawless countdown culminates with an on-time liftoff as the Space Shuttle Endeavour lights up the morning sky. Endeavour was launched on Mission STS-77 from Pad 39B at 6:30:00 a.m. EDT, May 19. The fourth Shuttle mission of 1996 is devoted to the continuing effort to help open the commercial space frontier. Heading up the six-member crew is Commander John H. Casper. Curtis L. Brown Jr. is the pilot and there are four mission specialists on board: Daniel W. Bursch, Mario Runco Jr., Andrew S. W. Thomas and Marc Garneau, who represents the Canadian Space Agency. During the approximately 10-day mission, the astronauts will perform a variety of payload activities, including microgravity research aboard the SPACEHAB-4 module, deployment and retrieval of the Spartan 207 carrying the Inflatable Antenna Experiment (IAE) and deployment and rendezvous with the Passive Aerodynamically-Stabilized Magnetically-Damped Satellite (PAMS).

Expression of the yeast NADH dehydrogenase Ndi1 in Drosophila confers increased lifespan independently of dietary restriction

PubMed Central

Sanz, Alberto; Soikkeli, Mikko; Portero-Otín, Manuel; Wilson, Angela; Kemppainen, Esko; McIlroy, George; Ellilä, Simo; Kemppainen, Kia K.; Tuomela, Tea; Lakanmaa, Matti; Kiviranta, Essi; Stefanatos, Rhoda; Dufour, Eric; Hutz, Bettina; Naudí, Alba; Jové, Mariona; Zeb, Akbar; Vartiainen, Suvi; Matsuno-Yagi, Akemi; Yagi, Takao; Rustin, Pierre; Pamplona, Reinald; Jacobs, Howard T.

2010-01-01

Mutations in mitochondrial oxidative phosphorylation complex I are associated with multiple pathologies, and complex I has been proposed as a crucial regulator of animal longevity. In yeast, the single-subunit NADH dehydrogenase Ndi1 serves as a non-proton-translocating alternative enzyme that replaces complex I, bringing about the reoxidation of intramitochondrial NADH. We have created transgenic strains of Drosophila that express yeast NDI1 ubiquitously. Mitochondrial extracts from NDI1-expressing flies displayed a rotenone-insensitive NADH dehydrogenase activity, and functionality of the enzyme in vivo was confirmed by the rescue of lethality resulting from RNAi knockdown of complex I. NDI1 expression increased median, mean, and maximum lifespan independently of dietary restriction, and with no change in sirtuin activity. NDI1 expression mitigated the aging associated decline in respiratory capacity and the accompanying increase in mitochondrial reactive oxygen species production, and resulted in decreased accumulation of markers of oxidative damage in aged flies. Our results support a central role of mitochondrial oxidative phosphorylation complex I in influencing longevity via oxidative stress, independently of pathways connected to nutrition and growth signaling. PMID:20435911

Converting NADH to NAD+ by nicotinamide nucleotide transhydrogenase as a novel strategy against mitochondrial pathologies during aging.

PubMed

Olgun, Abdullah

2009-08-01

Mitochondrial DNA defects are involved supposedly via free radicals in many pathologies including aging and cancer. But, interestingly, free radical production was not found increased in prematurely aging mice having higher mutation rate in mtDNA. Therefore, some other mechanisms like the increase of mitochondrial NADH/NAD(+) and ubiquinol/ubiquinone ratios, can be in action in respiratory chain defects. NADH/NAD(+) ratio can be normalized by the activation or overexpression of nicotinamide nucleotide transhydrogenase (NNT), a mitochondrial enzyme catalyzing the following very important reaction: NADH + NADP(+ )<--> NADPH + NAD(+). The products NAD(+) and NADPH are required in many critical biological processes, e.g., NAD(+) is used by histone deacetylase Sir2 which regulates longevity in different species. NADPH is used in a number of biosynthesis reactions (e.g., reduced glutathione synthesis), and processes like apoptosis. Increased ubiquinol/ubiquinone ratio interferes the function of dihydroorotate dehydrogenase, the only mitochondrial enzyme involved in ubiquinone mediated de novo pyrimidine synthesis. Uridine and its prodrug triacetyluridine are used to compensate pyrimidine deficiency but their bioavailability is limited. Therefore, the normalization of the ubiquinol/ubiquinone ratio can be accomplished by allotopic expression of alternative oxidase, a mitochondrial ubiquinol oxidase which converts ubiquinol to ubiquinone.

Space Shuttle Projects

NASA Image and Video Library

1984-04-24

The official mission insignia for the 41-D Space Shuttle flight features the Discovery - NASA's third orbital vehicle - as it makes its maiden voyage. The ghost ship represents the orbiter's namesakes which have figured prominently in the history of exploration. The Space Shuttle Discovery heads for new horizons to extend that proud tradition. Surnames for the crewmembers of NASA's eleventh Space Shuttle mission encircle the red, white, and blue scene.

The alternative NADH dehydrogenase is present in mitochondria of some animal taxa.

PubMed

Matus-Ortega, Macario Genaro; Salmerón-Santiago, Karina Gabriela; Flores-Herrera, Oscar; Guerra-Sánchez, Guadalupe; Martínez, Federico; Rendón, Juan Luis; Pardo, Juan Pablo

2011-09-01

The distribution of the alternative NADH dehydrogenase (NDH-2) in the living world was explored. The enzyme, although present in representatives of all living kingdoms, does not have a universal distribution. With the exception of ε-proteobacteria, the enzyme was found in all eubacterial groups. In contrast with the known presence of the NDH-2 in Archaea, the alternative oxidase (AOX) is absent in this group. With regard to the Eukarya domain, the NDH-2 was found in representatives of Protista, Fungi, Plantae, and Animalia. In the latter, however, the presence of the enzyme was restricted to some primitive Metazoa (Placozoa and Cnidaria), and two members of the Deuterostomate lineage of the Bilateria (Echinodermata and Urochordata). No evidence for the presence of the NDH-2 was found in any representative of the Protostomate branch of the Bilateria, contrasting with the existence of the AOX in this same group. It is worth mentioning that those animal species containing the NDH-2 also have an AOX. The actual distribution of the NDH-2 in the various living kingdoms is discussed within the framework of the endosymbiotic theory; in addition, a hypothesis is proposed to explain the disappearance of the alternative NDH-2 and AOX from the majority of the animals. Copyright © 2011 Elsevier Inc. All rights reserved.

Space Shuttle Projects

NASA Image and Video Library

1977-02-01

This photograph shows an inside view of a liquid hydrogen tank for the Space Shuttle external tank (ET) Main Propulsion Test Article (MPTA). The ET provides liquid hydrogen and liquid oxygen to the Shuttle's three main engines during the first 8.5 minutes of flight. At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and is the only part of the vehicle that is not reusable. The ET is manufactured at the Michoud Assembly Facility near New Orleans, Louisiana, by the Martin Marietta Corporation under management of the Marshall Space Flight Center.

Space Shuttle Projects

NASA Image and Video Library

1978-05-01

This photograph shows a liquid oxygen tank for the Shuttle External Tank (ET) during a hydroelastic modal survey test at the Marshall Space Flight Center. The ET provides liquid hydrogen and liquid oxygen to the Shuttle's three main engines during the first 8.5 minutes of flight. At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and is the only part of the vehicle that is not reusable. The ET is manufactured at the Michoud Assembly Facility near New Orleans, Louisiana, by the Martin Marietta Corporation under management of the Marshall Space Flight Center.

Aboard the mid-deck of the Earth-orbiting Space Shuttle Columbia, astronaut Charles J. Brady,

NASA Technical Reports Server (NTRS)

1996-01-01

STS-78 ONBOARD VIEW --- Aboard the mid-deck of the Earth-orbiting Space Shuttle Columbia, astronaut Charles J. Brady, mission specialist and a licensed amateur radio operator or ham, talks to students on Earth. Some of the crew members devoted some of their off-duty time to continue a long-standing Shuttle tradition of communicating with students and other hams between their shifts of assigned duty. Brady joined four other NASA astronauts and two international payload specialists for almost 17-days of research in support of the Life and Microgravity Spacelab (LMS-1) mission.

Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein-engineered NADH-preferring xylose reductase from Pichia stipitis.

PubMed

Watanabe, Seiya; Abu Saleh, Ahmed; Pack, Seung Pil; Annaluru, Narayana; Kodaki, Tsutomu; Makino, Keisuke

2007-09-01

A recombinant Saccharomyces cerevisiae strain transformed with xylose reductase (XR) and xylitol dehydrogenase (XDH) genes from Pichia stipitis (PsXR and PsXDH, respectively) has the ability to convert xylose to ethanol together with the unfavourable excretion of xylitol, which may be due to intercellular redox imbalance caused by the different coenzyme specificity between NADPH-preferring XR and NAD(+)-dependent XDH. In this study, we focused on the effect(s) of mutated NADH-preferring PsXR in fermentation. The R276H and K270R/N272D mutants were improved 52- and 146-fold, respectively, in the ratio of NADH/NADPH in catalytic efficiency [(k(cat)/K(m) with NADH)/(k(cat)/K(m) with NADPH)] compared with the wild-type (WT), which was due to decrease of k(cat) with NADPH in the R276H mutant and increase of K(m) with NADPH in the K270R/N272D mutant. Furthermore, R276H mutation led to significant thermostabilization in PsXR. The most positive effect on xylose fermentation to ethanol was found by using the Y-R276H strain, expressing PsXR R276H mutant and PsXDH WT: 20 % increase of ethanol production and 52 % decrease of xylitol excretion, compared with the Y-WT strain expressing PsXR WT and PsXDH WT. Measurement of intracellular coenzyme concentrations suggested that maintenance of the of NADPH/NADP(+) and NADH/NAD(+) ratios is important for efficient ethanol fermentation from xylose by recombinant S. cerevisiae.

Shuttle interaction study extension

NASA Technical Reports Server (NTRS)

1981-01-01

The implications of using the Shuttle with the SOC were analyzed, including constraints that the Shuttle places upon the SOC design. All the considerations involved in the use of the shuttle as a part of the SOC concept were identified.

Pilot Susan L. Still chats with white room closeout crew member

NASA Technical Reports Server (NTRS)

1997-01-01

KENNEDY SPACE CENTER, FLA. -- STS-83 Pilot Susan L. Still chats with white room closeout crew member Rene Arriens as she prepares to enter the Space Shuttle Columbia at Launch Pad 39A with assistance from closeout crew worker Bob Saulnier (behind Still).

Human sperm NADH and NADPH diaphorase cytochemistry: correlation with sperm motility.

PubMed

Zini, A; O'Bryan, M K; Israel, L; Schlegel, P N

1998-03-01

We have examined the correlation between the retention of residual sperm cytoplasm and sperm motility in semen from men presenting for infertility evaluation. Semen samples (n = 12) were obtained from nonazoospermic men presenting for infertility evaluation at our institution. Samples were fractionated into high-, intermediate-, and low-density subpopulations by Percoll gradients in order to examine the correlation between the retention of residual sperm cytoplasm and sperm motility. Residual sperm cytoplasm retention was detected by cytochemical staining of sperm for nicotinamide adenine dinucleotide (NADH)- or nicotinamide adenine dinucleotide phosphate (NADPH)-dependent diaphorase activity. The different sperm subpopulations (low, intermediate, and high density) had significantly different percentages of sperm with droplet retention (analysis of variance, P < 0.05). Using either NADH or NADPH diaphorase staining as a marker of the cytoplasmic space, a significant negative correlation was observed between the percentage of sperm with residual cytoplasmic droplets and the percentage of motile sperm (r = -0.58 and -0.61, respectively, P < 0.05). Assessment of residual sperm cytoplasm retention is a simple diagnostic test. Although this test is of unproven value in the management of infertile men, this and other studies suggest that it may provide useful data on sperm function.

[Involvement of hydrogen peroxide in the regulation of coexpression of alternative oxidase and rotenone-insensitive NADH dehydrogenase in tomato leaves and calluses].

PubMed

Eprintsev, A T; Mal'tseva, E V; Shatskikh, A S; Popov, V N

2011-01-01

The involvement of active oxygen forms in the regulation of the expression of mitochondrial respiratory chain components, which are not related to energy storing, has been in vitro and in vivo studied in Lycopersicum esculentum L. The highest level of transcription of genes encoding alternative oxidase and NADH dehydrogenase has been observed in green tomato leaves. It has been shown that even low H2O2 concentrations activate both aoxlalpha and ndb1 genes, encoding alternative oxidase and external mitochondrial rotenone-insensitive NADH dehydrogenase, respectively. According to our results, in the case of an oxidative stress, alternative oxidase and NADH dehydrogenase are coexpressed in tomato plant tissues, and active oxygen forms serve as the secondary messengers of their coexpression.

7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS AND PARKING LOT AREA. LOOKING WNW. GIS: N-37 40 36.2 / W-119 44 45.0 - Yosemite National Park Roads & Bridges, Yosemite Village, Mariposa County, CA

NASA newsletters for the Weber Student Shuttle Involvement Project

NASA Technical Reports Server (NTRS)

Morey-Holton, E. R.; Sebesta, P. D.; Ladwig, A. M.; Jackson, J. T.; Knott, W. M., III

1988-01-01

Biweekly reports generated for the Weber Student Shuttle Involvement Project (SSIP) are discussed. The reports document the evolution of science, hardware, and logistics for this Shuttle project aboard the eleventh flight of the Space Transportation System (STS-41B), launched from Kennedy Space Center on February 3, 1984, and returned to KSC 8 days later. The reports were intended to keep all members of the team aware of progress in the project and to avoid redundancy and misunderstanding. Since the Weber SSIP was NASA's first orbital rat project, documentation of all actions was essential to assure the success of this complex project. Eleven reports were generated: October 3, 17 and 31; November 14 and 28; and December 12 and 17, 1983; and January 3, 16, and 23; and May 1, 1984. A subject index of the reports is included. The final report of the project is included as an appendix.

A probe for NADH and H2O2 amperometric detection at low applied potential for oxidase and dehydrogenase based biosensor applications.

PubMed

Ricci, Francesco; Amine, Aziz; Moscone, Danila; Palleschi, Giuseppe

2007-01-15

Modified screen-printed electrodes for amperometric detection of H(2)O(2) and nicotinamide adenine dinucleotide (NADH) at low applied potential are presented in this paper. The sensors are obtained by modifying the working electrode surface with Prussian Blue, a well known electrochemical mediator for H(2)O(2) reduction. The coupling of this sensor with phenazine methosulfate (PMS) in the working solution gives the possibility of measuring both NAD(P)H and H(2)O(2). PMS reacts with NADH producing PMSH, which in the presence of oxygen, gives an equimolar amount of H(2)O(2). This allows the measurement of both analytes with similar sensitivity (357 mA mol(-1)L cm(-2) for H(2)O(2) and 336 mA mol(-1)L cm(-2) for NADH) and LOD (5x10(-7)mol L(-1) for H(2)O(2) and NADH) and opens the possibility of a whole series of biosensor applications. In this paper, results obtained with a variety of dehydrogenase enzymes (alcohol, malic, lactate, glucose, glycerol and glutamate) for the detection of enzymatic substrates or enzymatic activity are presented demonstrating the suitability of the proposed method for future biosensor applications.

Space Shuttle Projects

NASA Image and Video Library

1977-03-01

This photograph shows the liquid hydrogen tank and liquid oxygen tank for the Space Shuttle external tank (ET) being assembled in the weld assembly area of the Michoud Assembly Facility (MAF). The ET provides liquid hydrogen and liquid oxygen to the Shuttle's three main engines during the first eight 8.5 minutes of flight. At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and the only part of the vehicle that is not reusable. The ET is manufactured at the Michoud Assembly Facility near New Orleans, Louisiana, by the Martin Marietta Corporation under management of the Marshall Space Flight Center.

Space Shuttle Discovery Launch

NASA Image and Video Library

2008-05-31

NASA Shuttle Launch Director Michael Leinbach, left, STS-124 Assistant Launch Director Ed Mango, center, and Flow Director for Space Shuttle Discovery Stephanie Stilson clap in the the Launch Control Center after the main engine cut off and successful launch of the Space Shuttle Discovery (STS-124) Saturday, May 31, 2008, at the Kennedy Space Center in Cape Canaveral, Fla. The Shuttle lifted off from launch pad 39A at 5:02 p.m. EDT. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Debris Transport

NASA Technical Reports Server (NTRS)

Gomez, Reynaldo J., III

2010-01-01

This slide presentation reviews the assessment of debris damage to the Space Shuttle, and the use of computation to assist in the space shuttle applications. The presentation reviews the sources of debris, a mechanism for determining the probability of damaging debris impacting the shuttle, tools used, eliminating potential damaging debris sources, the use of computation to assess while inflight damage, and a chart showing the applications that have been used on increasingly powerful computers simulate the shuttle and the debris transport.

Effects of L-malate on physical stamina and activities of enzymes related to the malate-aspartate shuttle in liver of mice.

PubMed

Wu, J L; Wu, Q P; Huang, J M; Chen, R; Cai, M; Tan, J B

2007-01-01

L-malate, a tricarboxylic acid cycle (TCA) intermediate, plays an important role in transporting NADH from cytosol to mitochondria for energy production and may be involved in the beneficial effects of improving physical stamina. In the present study, we investigated the effects of L-malate on the performance of forced swimming time and blood biochemical parameters related to fatigue - blood urea nitrogen (BUN), glucose (Glc), creatine kinase (CK),total protein (TP) and lactic acid (LA). To investigate the effects of L-malate on the malate-aspartate shuttle and energy metabolism in mice, the activities of enzymes related to the malate-aspartate shuttle were measured. L-malate was orally administered to mice continuously for 30 days using a feeding atraumatic needle. The swimming time was increased by 26.1 % and 28.5 %, respectively, in the 0.210 g/kg and 0.630 g/kg L-malate-treated group compared with the control group. There were no differences in the concentrations of Glc, BUN and TP between the L-malate-treated groups and the control groups. However, the levels of CK were significantly decreased in the L-malate-treated groups. The results predict a potential benefit of L-malate for improving physical stamina and minimizing muscle damage during swimming exercise. The activities of cytosolic and mitochondrial malate dehydrogenase were significantly elevated in the L-malate-treated group compared with the control group. These enzymatic activities may be useful indicators for evaluating changes affecting the malate-aspartate shuttle and energy metabolism in the liver of mice.

Visitors during STS-132 Space Shuttle Atlantis Launch

NASA Image and Video Library

2010-05-14

STS132-S-013 (14 May 2010) --- As visitors watch, the space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Ben Cooper

Visitors during STS-132 Space Shuttle Atlantis Launch

NASA Image and Video Library

2010-05-14

STS132-S-014 (14 May 2010) --- With visitors looking on, the space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Ben Cooper

A microplate reader-based method to quantify NADH-cytochrome b5 reductase activity for diagnosis of recessive congenital methaemoglobinemia.

PubMed

Kedar, Prabhakar; Desai, Anand; Warang, Prashant; Colah, Roshan

2017-05-01

Congenital methemoglobinemia due to NADH-cytochrome b5 reductase 3 (CYB5R3) deficiencies is an autosomal recessive disorder that occurs sporadically worldwide, A sensitive, accurate, and rapid analysis of NADH-CYB5R enzyme concentrations is necessary for the diagnosis of RCM. Here we present an alternative microplate method that is based on a standard 96-well microplate format and microplate reader that simplify the quantification of NADH-CYB5R activity. TECAN (Infinite 200 PRO series) microplate reader with Tecan's proven Magellan™ software measured the NADH-CYB5R enzyme activity in 250 normal controls and previously diagnosed 25 cases of RCM due to NADH-CYB5R deficiency in the Indian population using 96-well microplates using 200 μl of total reaction mixture and also compared with standard spectrophotometric assay. We have also studied stability of the hemolysate stored at 4 and -20°C temperature. Enzyme activity in all 25 samples ranged from 6.09 to 10.07 IU/g Hb (mean ± SD: 8.08 ± 1.99 IU/g Hb) where as normal control ranged (n = 250) between 13.42 and 21.58 IU/g Hb) (mean ± SD: 17.5 ± 4.08 IU/g of Hb). Data obtained from the microplate reader were compared with standard spectrophotometer method and found 100% concordance using both methods. Microplate method allows differentiating between normal, deficient and intermediate enzyme activity. It was observed that samples had significant loss of activity when stored at 4°C and retained stable activity at -20°C for 1 week time. Our new method, incorporating a whole process of enzyme assay into a microplate format is readily applicable and allows rapid monitoring of enzyme assay. It is readily applicable to quantitative assay on pediatric sample as well as large number of samples for population screening.

Photochemical Properties and Reactivity of a Ru Compound Containing an NAD/NADH-Functionalized 1,10-Phenanthroline Ligand.

PubMed

Kobayashi, Katsuaki; Ohtsu, Hideki; Nozaki, Koichi; Kitagawa, Susumu; Tanaka, Koji

2016-03-07

An NAD/NADH-functionalized ligand, benzo[b]pyrido[3,2-f][1,7]-phenanthroline (bpp), was newly synthesized. A Ru compound containing the bpp ligand, [Ru(bpp)(bpy)2](2+), underwent 2e(-) and 2H(+) reduction, generating the NADH form of the compound, [Ru(bppHH)(bpy)2](2+), in response to visible light irradiation in CH3CN/TEA/H2O (8/1/1). The UV-vis and fluorescent spectra of both [Ru(bpp)(bpy)2](2+) and [Ru(bppHH)(bpy)2](2+) resembled the spectra of [Ru(bpy)3](2+). Both complexes exhibited strong emission, with quantum yields of 0.086 and 0.031, respectively; values that are much higher than those obtained from the NAD/NADH-functionalized complexes [Ru(pbn)(bpy)2](2+) and [Ru(pbnHH)(bpy)2](2+) (pbn = (2-(2-pyridyl)benzo[b]-1.5-naphthyridine, pbnHH = hydrogenated form of pbn). The reduction potential of the bpp ligand in [Ru(bpp)(bpy)2](2+) (-1.28 V vs SCE) is much more negative than that of the pbn ligand in [Ru(pbn)(bpy)2](2+) (-0.74 V), although the oxidation potentials of bppHH and pbnHH are essentially equal (0.95 V). These results indicate that the electrochemical oxidation of the dihydropyridine moiety in the NADH-type ligand was independent of the π system, including the Ru polypyridyl framework. [Ru(bppHH)(bpy)2](2+) allowed the photoreduction of oxygen, generating H2O2 in 92% yield based on [Ru(bppHH)(bpy)2](2+). H2O2 production took place via singlet oxygen generated by the energy transfer from excited [Ru(bppHH)(bpy)2](2+) to triplet oxygen.

History of Space Shuttle Rendezvous

NASA Technical Reports Server (NTRS)

Goodman, John L.

2011-01-01

This technical history is intended to provide a technical audience with an introduction to the rendezvous and proximity operations history of the Space Shuttle Program. It details the programmatic constraints and technical challenges encountered during shuttle development in the 1970s and over thirty years of shuttle missions. An overview of rendezvous and proximity operations on many shuttle missions is provided, as well as how some shuttle rendezvous and proximity operations systems and flight techniques evolved to meet new programmatic objectives. This revised edition provides additional information on Mercury, Gemini, Apollo, Skylab, and Apollo/Soyuz. Some chapters on the Space Shuttle have been updated and expanded. Four special focus chapters have been added to provide more detailed information on shuttle rendezvous. A chapter on the STS-39 mission of April/May 1991 describes the most complex deploy/retrieve mission flown by the shuttle. Another chapter focuses on the Hubble Space Telescope servicing missions. A third chapter gives the reader a detailed look at the February 2010 STS-130 mission to the International Space Station. The fourth chapter answers the question why rendezvous was not completely automated on the Gemini, Apollo, and Space Shuttle vehicles.

Vesicle encapsulation of a nonbiological photochemical system capable of reducing NAD(+) to NADH.

PubMed

Summers, David P; Rodoni, David

2015-10-06

One of the fundamental structures of a cell is the membrane. Self-assembling lipid bilayer vesicles can form the membrane of an artificial cell and could also have plausibly assembled prebiotically for the origin of life. Such cell-like structures, that encapsulate some basic subset of the functions of living cells, are important for research to infer the minimum chemistry necessary for a cell, to help understand the origin of life, and to allow the production of useful species in microscopic containers. We show that the encapsulation of TiO2 particles has the potential to provide the basis for an energy transduction system inside vesicles which can be used to drive subsequent chemistry. TiO2 encapsulated in vesicles can be used to produce biochemical species such as NADH. The NADH is formed from NAD(+) reduction and is produced in a form that is able to drive further enzymatic chemistry. This allows us to link a mineral-based, nonbiological photosystem to biochemical reactions. This is a fundamental step toward being able to use this mineral photosystem in a protocell/artificial cell.

Space Shuttle Projects

NASA Image and Video Library

1992-05-13

STS-49, the first flight of the Space Shuttle Orbiter Endeavour, lifted off from launch pad 39B on May 7, 1992 at 6:40 pm CDT. The STS-49 mission was the first U.S. orbital flight to feature 4 extravehicular activities (EVAs), and the first flight to involve 3 crew members working simultaneously outside of the spacecraft. The primary objective was the capture and redeployment of the INTELSAT VI (F-3), a communication satellite for the International Telecommunication Satellite organization, which was stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. The 4.5 ton satellite was successfully snared by three astronauts on a third EVA. The three hand-grabbed the errant satellite, pulled it into the cargo bay, and attached a boost-given perigee stage before its release. In this photo, the satellite spins slowly out of cargo bay to begin its “new lift”.

Shuttle communications design study

NASA Technical Reports Server (NTRS)

Cartier, D. E.

1975-01-01

The design and development of a space shuttle communication system are discussed. The subjects considered include the following: (1) Ku-band satellite relay to shuttle, (2) phased arrays, (3) PN acquisition, (4) quadriplexing of direct link ranging and telemetry, (5) communications blackout on launch and reentry, (6) acquisition after blackout on reentry, (7) wideband communications interface with the Ku-Band rendezvous radar, (8) aeroflight capabilities of the space shuttle, (9) a triple multiplexing scheme equivalent to interplex, and (10) a study of staggered quadriphase for use on the space shuttle.

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

The space shuttle Endeavour is seen as it traverses through Inglewood, Calif. on Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Projects

NASA Image and Video Library

2002-03-03

This is a photo of the Hubble Space Telescope (HST),in its origianl configuration, berthed in the cargo bay of the Space Shuttle Columbia during the STS-109 mission silhouetted against the airglow of the Earth's horizon. The telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm, where 4 of the 7-member crew performed 5 spacewalks completing system upgrades to the HST. Included in those upgrades were: replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when its original coolant ran out. The Marshall Space Flight Center had the responsibility for the design, development, and construction of the the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. The HST detects objects 25 times fainter than the dimmest objects seen from Earth and provides astronomers with an observable universe 250 times larger than is visible from ground-based telescopes, perhaps as far away as 14 billion light-years. The HST views galaxies, stars, planets, comets, possibly other solar systems, and even unusual phenomena such as quasars, with 10 times the clarity of ground-based telescopes. Launched March 1, 2002 the STS-109 HST servicing mission lasted 10 days, 22 hours, and 11 minutes. It was the 108th flight overall in NASA's Space Shuttle Program.

Space Shuttle Projects

NASA Image and Video Library

2002-03-01

Carrying a crew of seven, the Space Shuttle Orbiter Columbia soared through some pre-dawn clouds into the sky as it began its 27th flight, STS-109. Launched March 1, 2002, the goal of the mission was the maintenance and upgrade of the Hubble Space Telescope (HST). The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. The HST detects objects 25 times fainter than the dimmest objects seen from Earth and provides astronomers with an observable universe 250 times larger than is visible from ground-based telescopes, perhaps as far away as 14 billion light-years. The HST views galaxies, stars, planets, comets, possibly other solar systems, and even unusual phenomena such as quasars, with 10 times the clarity of ground-based telescopes. During the STS-109 mission, the telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm. Here four members of the crew performed five spacewalks completing system upgrades to the HST. Included in those upgrades were: replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when it original coolant ran out. Lasting 10 days, 22 hours, and 11 minutes, the STS-109 mission was the 108th flight overall in NASA's Space Shuttle Program.

Bioinspired Design of Alcohol Dehydrogenase@nano TiO₂ Microreactors for Sustainable Cycling of NAD⁺/NADH Coenzyme.

PubMed

Lin, Sen; Sun, Shiyong; Wang, Ke; Shen, Kexuan; Ma, Biaobiao; Ren, Yuquan; Fan, Xiaoyu

2018-02-24

The bioinspired design and construction of enzyme@capsule microreactors with specific cell-like functionality has generated tremendous interest in recent years. Inspired by their fascinating complexity, scientists have endeavored to understand the essential aspects of a natural cell and create biomimicking microreactors so as to immobilize enzymes within the hierarchical structure of a microcapsule. In this study, simultaneous encapsulation of alcohol dehydrogenase (ADH) was achieved during the preparation of microcapsules by the Pickering emulsion method using amphiphilic modified TiO₂ nanoparticles (NPs) as building blocks for assembling the photocatalytic microcapsule membrane. The ADH@TiO₂ NP microreactors exhibited dual catalytic functions, i.e., spatially confined enzymatic catalysis and the membrane-associated photocatalytic oxidation under visible light. The sustainable cycling of nicotinamide adenine dinucleotide (NAD) coenzyme between NADH and NAD⁺ was realized by enzymatic regeneration of NADH from NAD⁺ reduction, and was provided in a form that enabled further photocatalytic oxidation to NAD⁺ under visible light. This bioinspired ADH@TiO₂ NP microreactor allowed the linking of a semiconductor mineral-based inorganic photosystem to enzymatic reactions. This is a first step toward the realization of sustainable biological cycling of NAD⁺/NADH coenzyme in synthetic functional microsystems operating under visible light irradiation.

Does Oral Coenzyme Q10 Plus NADH Supplementation Improve Fatigue and Biochemical Parameters in Chronic Fatigue Syndrome?

PubMed Central

Cordero, Mario D.; Segundo, María José; Sáez-Francàs, Naia; Calvo, Natalia; Román-Malo, Lourdes; Aliste, Luisa; Fernández de Sevilla, Tomás; Alegre, José

2015-01-01

Abstract Chronic fatigue syndrome (CFS) is a chronic and extremely debilitating illness characterized by prolonged fatigue and multiple symptoms with unknown cause, diagnostic test, or universally effective treatment. Inflammation, oxidative stress, mitochondrial dysfunction, and CoQ10 deficiency have been well documented in CFS. We conducted an 8-week, randomized, double-blind placebo-controlled trial to evaluate the benefits of oral CoQ10 (200 mg/day) plus NADH (20 mg/day) supplementation on fatigue and biochemical parameters in 73 Spanish CFS patients. This study was registered in ClinicalTrials.gov (NCT02063126). A significant improvement of fatigue showing a reduction in fatigue impact scale total score (p<0.05) was reported in treated group versus placebo. In addition, a recovery of the biochemical parameters was also reported. NAD+/NADH (p<0.001), CoQ10 (p<0.05), ATP (p<0.05), and citrate synthase (p<0.05) were significantly higher, and lipoperoxides (p<0.05) were significantly lower in blood mononuclear cells of the treated group. These observations lead to the hypothesis that the oral CoQ10 plus NADH supplementation could confer potential therapeutic benefits on fatigue and biochemical parameters in CFS. Larger sample trials are warranted to confirm these findings. Antioxid. Redox Signal. 22, 679–685. PMID:25386668

McGill-trained MD, experiment give June 20 shuttle flight strong Canadian flavour.

PubMed Central

Thirsk, R

1996-01-01

Family physician Robert Thirsk, an original member of the Canadian Space Agency's astronaut program, will be part of the seven-member crew when the space shuttle Columbia lifts off from Florida's Kennedy Space Centre June 20. In this special report, the 1982 McGill graduate outlines some of the physiologic and materials-science experiments the crew will conduct. Thirsk, a payload specialist and crew medical officer, thinks the findings could have a significant impact on future space missions, medicine and the biotechnology industry. Images p1885-a p1887-a PMID:8653649

STS-84 crew members in M-113 armored carrier for TCDT

NASA Technical Reports Server (NTRS)

1997-01-01

STS-84 crew members ride in and learn how to operate an M-113 armored personnel carrier as part of the Terminal Countdown Demonstration Test (TCDT) activities. Seated inside the M-113, from left, are Mission Specialist Jean-Francois Clervoy, Pilot Eileen Marie Collins (waving) and Commander Charles J. Precourt, in front. George Hoggard, a training officer with KSC Fire Services, sits on top of the personnel carrier. STS-84 aboard the Space Shuttle Atlantis will be the sixth docking of the Space Shuttle with the Russian Space Station Mir. After docking, STS-84 Mission Specialist C. Michael Foale will transfer to the space station and become a member of the Mir 23 crew, replacing U.S. astronaut Jerry M. Linenger, who will return to Earth aboard Atlantis. Foale will live and work on Mir until mid-September when his replacement is expected to arrive on the STS-86 mission. STS-84 is targeted for a May 15 liftoff.

STS-86 crew members Lawrence, Titov and Parazynski in M-113

NASA Technical Reports Server (NTRS)

1997-01-01

STS-86 crew members get a ride in, and learn to operate, an M-113 armored personnel carrier as part of training exercises during the Terminal Countdown Demonstration Test (TCDT), a dress rehearsal for launch. George Hoggard, in back at left, a training officer with KSC Fire Services, provides this part of the training to Mission Specialists David A. Wolf, to the right of Hoggard; Jean-Loup J.M. Chretien of the French Space Agency; and Vladimir Georgievich Titov, in foreground, of the Russian Space Agency. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. During the docking, Wolf will transfer to the orbiting Russian station and become a member of the Mir 24 crew, replacing U.S. astronaut C. Michael Foale, who has been on the Mir since the last docking mission, STS-84, in May. Launch of Mission STS-86 aboard the Space Shuttle Atlantis is targeted for Sept. 25 from Launch Pad 39A.

NqrM (DUF539) Protein Is Required for Maturation of Bacterial Na+-Translocating NADH:Quinone Oxidoreductase

PubMed Central

Kostyrko, Vitaly A.; Bertsova, Yulia V.; Serebryakova, Marina V.; Baykov, Alexander A.

2015-01-01

ABSTRACT Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) catalyzes electron transfer from NADH to ubiquinone in the bacterial respiratory chain, coupled with Na+ translocation across the membrane. Na+-NQR maturation involves covalent attachment of flavin mononucleotide (FMN) residues, catalyzed by flavin transferase encoded by the nqr-associated apbE gene. Analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrM) that usually follows the apbE gene and is present only in Na+-NQR-containing bacteria. Expression of the Vibrio harveyi nqr operon alone or with the associated apbE gene in Escherichia coli, which lacks its own Na+-NQR, resulted in an enzyme incapable of Na+-dependent NADH or reduced nicotinamide hypoxanthine dinucleotide (dNADH) oxidation. However, fully functional Na+-NQR was restored when these genes were coexpressed with the V. harveyi nqrM gene. Furthermore, nqrM lesions in Klebsiella pneumoniae and V. harveyi prevented production of functional Na+-NQR, which could be recovered by an nqrM-containing plasmid. The Na+-NQR complex isolated from the nqrM-deficient strain of V. harveyi lacks several subunits, indicating that nqrM is necessary for Na+-NQR assembly. The protein product of the nqrM gene, NqrM, contains a single putative transmembrane α-helix and four conserved Cys residues. Mutating one of these residues (Cys33 in V. harveyi NqrM) to Ser completely prevented Na+-NQR maturation, whereas mutating any other Cys residue only decreased the yield of the mature protein. These findings identify NqrM as the second specific maturation factor of Na+-NQR in proteobacteria, which is presumably involved in the delivery of Fe to form the (Cys)4[Fe] center between subunits NqrD and NqrE. IMPORTANCE Na+-translocating NADH:quinone oxidoreductase complex (Na+-NQR) is a unique primary Na+ pump believed to enhance the vitality of many bacteria, including important pathogens such as Vibrio cholerae, Vibrio

Isolation, Oxygen Sensitivity, and Virulence of NADH Oxidase Mutants of the Anaerobic Spirochete Brachyspira (Serpulina) hyodysenteriae, Etiologic Agent of Swine Dysentery

PubMed Central

Stanton, Thad B.; Rosey, Everett L.; Kennedy, Michael J.; Jensen, Neil S.; Bosworth, Brad T.

1999-01-01

Brachyspira (Serpulina) hyodysenteriae, the etiologic agent of swine dysentery, uses the enzyme NADH oxidase to consume oxygen. To investigate possible roles for NADH oxidase in the growth and virulence of this anaerobic spirochete, mutant strains deficient in oxidase activity were isolated and characterized. The cloned NADH oxidase gene (nox; GenBank accession no. U19610) on plasmid pER218 was inactivated by replacing 321 bp of coding sequence with either a gene for chloramphenicol resistance (cat) or a gene for kanamycin resistance (kan). The resulting plasmids, respectively, pCmΔNOX and pKmΔNOX, were used to transform wild-type B. hyodysenteriae B204 cells and generate the antibiotic-resistant strains Nox-Cm and Nox-Km. PCR and Southern hybridization analyses indicated that the chromosomal wild-type nox genes in these strains had been replaced, through allelic exchange, by the inactivated nox gene containing cat or kan. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblot analysis revealed that both nox mutant cell lysates were missing the 48-kDa Nox protein. Soluble NADH oxidase activity levels in cell lysates of Nox-Cm and Nox-Km were reduced 92 to 96% compared to the activity level in parent strain B204. In an aerotolerance test, cells of both nox mutants were at least 100-fold more sensitive to oxygen exposure than were cells of the wild-type parent strain B204. In swine experimental infections, both nox mutants were less virulent than strain B204 in that fewer animals were colonized by the mutant cells and infected animals displayed mild, transient signs of disease, with no deaths. These results provide evidence that NADH oxidase serves to protect B. hyodysenteriae cells against oxygen toxicity and that the enzyme, in that role, contributes to the pathogenic ability of the spirochete. PMID:10543819

Space Shuttle Project

NASA Image and Video Library

1981-01-01

A Space Shuttle Main Engine undergoes test-firing at the National Space Technology Laboratories (now the Sternis Space Center) in Mississippi. The Marshall Space Flight Center had management responsibility of Space Shuttle propulsion elements, including the Main Engines.

Space Shuttle Program

NASA Image and Video Library

2012-09-12

Ronnie Rigney (r), chief of the Propulsion Test Office in the Project Directorate at Stennis Space Center, stands with agency colleagues to receive the prestigious American Institute of Aeronautics and Astronautics George M. Low Space Transportation Award on Sept. 12. Rigney accepted the award on behalf of the NASA and contractor team at Stennis for their support of the Space Shuttle Program that ended last summer. From 1975 to 2009, Stennis Space Center tested every main engine used to power 135 space shuttle missions. Stennis continued to provide flight support services through the end of the Space Shuttle Program in July 2011. The center also supported transition and retirement of shuttle hardware and assets through September 2012. The 2012 award was presented to the space shuttle team 'for excellence in the conception, development, test, operation and retirement of the world's first and only reusable space transportation system.' Joining Rigney for the award ceremony at the 2012 AIAA Conference in Pasadena, Calif., were: (l to r) Allison Zuniga, NASA Headquarters; Michael Griffin, former NASA administrator; Don Noah, Johnson Space Center in Houston; Steve Cash, Marshall Space Flight Center in Huntsville, Ala.; and Pete Nickolenko, Kennedy Space Center in Florida.

Space Shuttle Projects

NASA Image and Video Library

2004-04-15

The Apollo program demonstrated that men could travel into space, perform useful tasks there, and return safely to Earth. But space had to be more accessible. This led to the development of the Space Shuttle. The Shuttle's major components are the orbiter spacecraft; the three main engines, with a combined thrust of more than 1.2 million pounds; the huge external tank (ET) that feeds the liquid hydrogen fuel and liquid oxygen oxidizer to the three main engines; and the two solid rocket boosters (SRBs), with their combined thrust of some 5.8 million pounds, that provide most of the power for the first two minutes of flight. Crucially involved with the Space Shuttle program virtually from its inception, the Marshall Space Flight Center (MSFC) played a leading role in the design, development, testing, and fabrication of many major Shuttle propulsion components.

Space Shuttle Drawing

NASA Technical Reports Server (NTRS)

2004-01-01

The Apollo program demonstrated that men could travel into space, perform useful tasks there, and return safely to Earth. But space had to be more accessible. This led to the development of the Space Shuttle. The Shuttle's major components are the orbiter spacecraft; the three main engines, with a combined thrust of more than 1.2 million pounds; the huge external tank (ET) that feeds the liquid hydrogen fuel and liquid oxygen oxidizer to the three main engines; and the two solid rocket boosters (SRBs), with their combined thrust of some 5.8 million pounds, that provide most of the power for the first two minutes of flight. Crucially involved with the Space Shuttle program virtually from its inception, the Marshall Space Flight Center (MSFC) played a leading role in the design, development, testing, and fabrication of many major Shuttle propulsion components.

NASA Facts, Space Shuttle.

ERIC Educational Resources Information Center

National Aeronautics and Space Administration, Washington, DC. Educational Programs Div.

This newsletter from the National Aeronautics and Space Administration (NASA) contains a description of the purposes and potentials of the Space Shuttle craft. The illustrated document explains some of the uses for which the shuttle is designed; how the shuttle will be launched from earth, carry out its mission, and land again on earth; and what a…

Space Shuttle Project

NASA Image and Video Library

1972-03-07

This early chart conceptualizes the use of two parallel Solid Rocket Motor Boosters in conjunction with three main engines to launch the proposed Space Shuttle to orbit. At approximately twenty-five miles altitude, the boosters would detach from the Orbiter and parachute back to Earth where they would be recovered and refurbished for future use. The Shuttle was designed as NASA's first reusable space vehicle, launching vertically like a spacecraft and landing on runways like conventional aircraft. Marshall Space Flight Center had management responsibility for the Shuttle's propulsion elements, including the Solid Rocket Boosters.

The plastid ndh genes code for an NADH-specific dehydrogenase: Isolation of a complex I analogue from pea thylakoid membranes

PubMed Central

Sazanov, Leonid A.; Burrows, Paul A.; Nixon, Peter J.

1998-01-01

The plastid genomes of several plants contain ndh genes—homologues of genes encoding subunits of the proton-pumping NADH:ubiquinone oxidoreductase, or complex I, involved in respiration in mitochondria and eubacteria. From sequence similarities with these genes, the ndh gene products have been suggested to form a large protein complex (Ndh complex); however, the structure and function of this complex remains to be established. Herein we report the isolation of the Ndh complex from the chloroplasts of the higher plant Pisum sativum. The purification procedure involved selective solubilization of the thylakoid membrane with dodecyl maltoside, followed by two anion-exchange chromatography steps and one size-exclusion chromatography step. The isolated Ndh complex has an apparent total molecular mass of approximately 550 kDa and according to SDS/PAGE consists of at least 16 subunits including NdhA, NdhI, NdhJ, NdhK, and NdhH, which were identified by N-terminal sequencing and immunoblotting. The Ndh complex showed an NADH- and deamino-NADH-specific dehydrogenase activity, characteristic of complex I, when either ferricyanide or the quinones menadione and duroquinone were used as electron acceptors. This study describes the isolation of the chloroplast analogue of the respiratory complex I and provides direct evidence for the function of the plastid Ndh complex as an NADH:plastoquinone oxidoreductase. Our results are compatible with a dual role for the Ndh complex in the chlororespiratory and cyclic photophosphorylation pathways. PMID:9448329

KENNEDY SPACE CENTER, FLA. - In the Columbia Debris Hangar, Shuttle Launch Director Mike Leinbach (left) talks to members of the Stafford-Covey Return to Flight Task Group (SCTG) about reconstruction efforts. Chairing the task group are Richard O. Covey (second from right), former Space Shuttle commander, and Thomas P. Stafford, Apollo commander. Chartered by NASA Administrator Sean O’Keefe, the task group will perform an independent assessment of NASA’s implementation of the final recommendations by the Columbia Accident Investigation Board.

NASA Image and Video Library

2003-08-05

KENNEDY SPACE CENTER, FLA. - In the Columbia Debris Hangar, Shuttle Launch Director Mike Leinbach (left) talks to members of the Stafford-Covey Return to Flight Task Group (SCTG) about reconstruction efforts. Chairing the task group are Richard O. Covey (second from right), former Space Shuttle commander, and Thomas P. Stafford, Apollo commander. Chartered by NASA Administrator Sean O’Keefe, the task group will perform an independent assessment of NASA’s implementation of the final recommendations by the Columbia Accident Investigation Board.

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

The space shuttle Enterprise is lowered onto a transport vehicle after being demated from the NASA 747 Shuttle Carrier Aircraft (SCA) at John F. Kennedy (JFK) International Airport in Jamica, New York, Sunday, May 13, 2012. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to the Intrepid Sea, Air & Space Museum in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

The space shuttle Enterprise hangs from a sling after being demated from the NASA 747 Shuttle Carrier Aircraft (SCA) at John F. Kennedy (JFK) International Airport in Jamica, New York, Sunday, May 13, 2012. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to the Intrepid Sea, Air & Space Museum in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

A yellow sling is lowered onto space shuttle Enterprise, which sits atop NASA's 747 Shuttle Carrier Aircraft (SCA) prior to it being demated a few hours later at John F. Kennedy (JFK) International Airport in New York, Saturday, May 12, 2012. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to Intrepid in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

Spectators watch space shuttle Endeavour as it passes by on its way to its new home at the California Science Center in Los Angeles, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

The space shuttle Endeavour is seen as it traverses through the streest of Los Angeles on its way to its new home at the California Science Center, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

A spectator photographs the space shuttle Endeavour as it passes by on its way to its new home at the California Science Center in Los Angeles, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

A spectator is seen photographing the space shuttle Endeavour as it is moved to its new home at the California Science Center in Los Angeles, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC’s Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Project

NASA Image and Video Library

1978-10-04

The Shuttle Orbiter Enterprise inside of Marshall Space Flight Center's Dynamic Test Stand for Mated Vertical Ground Vibration tests (MVGVT). The tests marked the first time ever that the entire shuttle complement including Orbiter, external tank, and solid rocket boosters were vertically mated.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-074 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-080 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-076 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-072 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-075 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-077 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-081 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-073 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-078 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-079 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Launch of Space Shuttle Atlantis STS-132

NASA Image and Video Library

2010-05-14

STS132-S-071 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

NASA's 747 Shuttle Carrier Aircraft (SCA), with space shuttle Enterprise latched on its back, is towed from the hangar at John F. Kennedy (JFK) International Airport in New York late in the night on Saturday, May 12, 2012. Early Sunday morning, Enterprise was removed from the SCA. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to Intrepid in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

The driver of the Over Land Transporter is seen as he maneuvers the space shuttle Endeavour on the streets of Los Angeles as it heads to its new home at the California Science Center, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC’s Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

Spectators are seen as they watch space shuttle Endeavour as it passes by on its way to its new home at the California Science Center in Los Angeles, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

The driver of the Over Land Transporter (OLT) is seen as he maneuvers the space shuttle Endeavour on the streets of Los Angeles as it heads to its new home at the California Science Center, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

A spectator on the roof of a building photographs space shuttle Endeavour as it passes by on its way to its new home at the California Science Center in Los Angeles, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC’s Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Endeavour Move

NASA Image and Video Library

2012-10-12

The space shuttle Endeavour moves out of the Los Angeles International Airport and onto the streets of Los Angeles to make its way to its new home at the California Science Center, Friday, Oct. 12, 2012. Endeavour, built as a replacement for space shuttle Challenger, completed 25 missions, spent 299 days in orbit, and orbited Earth 4,671 times while traveling 122,883,151 miles. Beginning Oct. 30, the shuttle will be on display in the CSC's Samuel Oschin Space Shuttle Endeavour Display Pavilion, embarking on its new mission to commemorate past achievements in space and educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

Space Shuttle Projects

NASA Image and Video Library

1978-03-01

A liquid hydrogen tank of the Shuttle's external tank (ET) is installed into the S-1C Test Stand for a structural test at the Marshall Space Flight Center. At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and is the only part of the vehicle that is not reusable. The ET is manufactured at the Michoud Assembly Facility near New Orleans, Louisiana, by the Martin Marietta Corporation under management of the Marshall Space Flight Center.

Fifth Report of the NASA Advisory Council Task Force on the Shuttle-Mir Rendezvous and Docking Missions

NASA Technical Reports Server (NTRS)

1995-01-01

The NASA Advisory Council Task Force on the Shuttle-Mir rendezvous and docking missions examine a number of specific issues related to the Shuttle-Mir program. Three teams composed of Task Force members and technical advisors were formed to address the follow issues: preliminary results from STS-71 and the status of preparations for STS-74; NASA's presence in Russia; and NASA's automated data processing and telecommunications (ADP/T) infrastructure in Russia. The three review team reports have been included in the fifth report of the Task Force.

Space Shuttle Strategic Planning Status

NASA Technical Reports Server (NTRS)

Henderson, Edward M.; Norbraten, Gordon L.

2006-01-01

The Space Shuttle Program is aggressively planning the Space Shuttle manifest for assembling the International Space Station and servicing the Hubble Space Telescope. Implementing this flight manifest while concurrently transitioning to the Exploration architecture creates formidable challenges; the most notable of which is retaining critical skills within the Shuttle Program workforce. The Program must define a strategy that will allow safe and efficient fly-out of the Shuttle, while smoothly transitioning Shuttle assets (both human and facility) to support early flight demonstrations required in the development of NASA s Crew Exploration Vehicle (CEV) and Crew and Cargo Launch Vehicles (CLV). The Program must accomplish all of this while maintaining the current level of resources. Therefore, it will be necessary to initiate major changes in operations and contracting. Overcoming these challenges will be essential for NASA to fly the Shuttle safely, accomplish the President s "Vision for Space Exploration," and ultimately meet the national goal of maintaining a robust space program. This paper will address the Space Shuttle Program s strategy and its current status in meeting these challenges.

Space Shuttle Strategic Planning Status

NASA Technical Reports Server (NTRS)

Norbraten, Gordon L.; Henderson, Edward M.

2007-01-01

The Space Shuttle Program is aggressively flying the Space Shuttle manifest for assembling the International Space Station and servicing the Hubble Space Telescope. Completing this flight manifest while concurrently transitioning to the Exploration architecture creates formidable challenges; the most notable of which is retaining critical skills within the Shuttle Program workforce. The Program must define a strategy that will allow safe and efficient fly-out of the Shuttle, while smoothly transitioning Shuttle assets (both human and facility) to support early flight demonstrations required in the development of NASA's Crew Exploration Vehicle (Orion) and Crew and Cargo Launch Vehicles (Ares I). The Program must accomplish all of this while maintaining the current level of resources. Therefore, it will be necessary to initiate major changes in operations and contracting. Overcoming these challenges will be essential for NASA to fly the Shuttle safely, accomplish the Vision for Space Exploration, and ultimately meet the national goal of maintaining a robust space program. This paper will address the Space Shuttle Program s strategy and its current status in meeting these challenges.

Space Shuttle Project

NASA Image and Video Library

1978-04-21

The Shuttle Orbiter Enterprise is lowered into the Dynamic Test Stand for Mated Vertical Ground Vibration tests (MVGVT) at the Marshall Space Flight Center. The tests marked the first time ever that the entire shuttle complement (including Orbiter, external tank, and solid rocket boosters) were mated vertically.

Shuttle Discovery Landing at Edwards

NASA Technical Reports Server (NTRS)

1989-01-01

The STS-29 Space Shuttle Discovery mission lands at NASA's then Ames-Dryden Flight Research Facility, Edwards AFB, California, early Saturday morning, 18 March 1989. Touchdown was at 6:35:49 a.m. PST and wheel stop was at 6:36:40 a.m. on runway 22. Controllers chose the concrete runway for the landing in order to make tests of braking and nosewheel steering. The STS-29 mission was very successful, completing the launch of a Tracking and Data Relay communications satellite, as well as a range of scientific experiments. Discovery's five-man crew was led by Commander Michael L. Coats, and included pilot John E. Blaha and mission specialists James P. Bagian, Robert C. Springer, and James F. Buchli. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout

Space Shuttle Columbia prepares to touch down on Runway 33

NASA Technical Reports Server (NTRS)

1997-01-01

KENNEDY SPACE CENTER, FLA. -- The Space Shuttle Columbia prepares to touch down on Runway 33 at KSC''';s Shuttle Landing Facility at approximately 2:33 p.m. EDT, April 8, to conclude the Microgravity Science Laboratory-1 (MSL-1) mission. At main gear touchdown, the STS-83 mission duration will be just under four days. The planned 16-day mission was cut short by a faulty fuel cell. This is only the third time in Shuttle program history that an orbiter was brought home early due to mechanical problems. This was also the 36th KSC landing since the program began in 1981. Mission Commander James D. Halsell, Jr. flew Columbia to a perfect landing with help from Pilot Susan L. Still. Other crew members are Payload Commander Janice E. Voss; Mission Specialists Michael L.Gernhardt and Donald A. Thomas; and Payload Specialists Roger K. Crouch and Gregory T. Linteris. In spite of the abbreviated flight, the crew was able to perform MSL-1 experiments. The Spacelab-module-based experiments were used to test some of the hardware, facilities and procedures that are planned for use on the International Space Station and to conduct combustion, protein crystal growth and materials processing investigations.

Nafuredin, a novel inhibitor of NADH-fumarate reductase, produced by Aspergillus niger FT-0554.

PubMed

Ui, H; Shiomi, K; Yamaguchi, Y; Masuma, R; Nagamitsu, T; Takano, D; Sunazuka, T; Namikoshi, M; Omura, S

2001-03-01

A novel compound, nafuredin, was isolated as an inhibitor of anaerobic electron transport (NADH-fumarate reductase). It was obtained from culture broth of Aspergillus niger FT-0554 isolated from a marine sponge. The structure was elucidated as an epoxy-delta-lactone with an attached methylated olefinic side chain on the basis of spectral analysis.

Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein.

PubMed

Zhang, Xiaomin; Azhar, Gohar; Helms, Scott; Zhong, Ying; Wei, Jeanne Y

2008-01-29

The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart. In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor. These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein

PubMed Central

Zhang, Xiaomin; Azhar, Gohar; Helms, Scott; Zhong, Ying; Wei, Jeanne Y

2008-01-01

Background The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart. Results In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor. Conclusion These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging. PMID:18230186

Space Shuttle Project

NASA Image and Video Library

1978-10-04

The Shuttle Orbiter Enterprise is being installed into liftoff configuration at Marshall Space Flight Center's Dynamic Test Stand for Mated Vertical Ground Vibration tests (MVGVT). The tests marked the first time ever that the entire shuttle complement (including Orbiter, external tank, and solid rocket boosters) were mated vertically.

Shuttle car loading system

NASA Technical Reports Server (NTRS)

Collins, E. R., Jr. (Inventor)

1985-01-01

A system is described for loading newly mined material such as coal, into a shuttle car, at a location near the mine face where there is only a limited height available for a loading system. The system includes a storage bin having several telescoping bin sections and a shuttle car having a bottom wall that can move under the bin. With the bin in an extended position and filled with coal the bin sections can be telescoped to allow the coal to drop out of the bin sections and into the shuttle car, to quickly load the car. The bin sections can then be extended, so they can be slowly filled with more while waiting another shuttle car.

Shuttle Wastewater Solution Characterization

NASA Technical Reports Server (NTRS)

Adam, Niklas; Pham, Chau

2011-01-01

During the 31st shuttle mission to the International Space Station, STS-129, there was a clogging event in the shuttle wastewater tank. A routine wastewater dump was performed during the mission and before the dump was completed, degraded flow was observed. In order to complete the wastewater dump, flow had to be rerouted around the dump filter. As a result, a basic chemical and microbial investigation was performed to understand the shuttle wastewater system and perform mitigation tasks to prevent another blockage. Testing continued on the remaining shuttle flights wastewater and wastewater tank cleaning solutions. The results of the analyses and the effect of the mitigation steps are detailed in this paper.

NADH-fluorescence scattering correction for absolute concentration determination in a liquid tissue phantom using a novel multispectral magnetic-resonance-imaging-compatible needle probe

NASA Astrophysics Data System (ADS)

Braun, Frank; Schalk, Robert; Heintz, Annabell; Feike, Patrick; Firmowski, Sebastian; Beuermann, Thomas; Methner, Frank-Jürgen; Kränzlin, Bettina; Gretz, Norbert; Rädle, Matthias

2017-07-01

In this report, a quantitative nicotinamide adenine dinucleotide hydrate (NADH) fluorescence measurement algorithm in a liquid tissue phantom using a fiber-optic needle probe is presented. To determine the absolute concentrations of NADH in this phantom, the fluorescence emission spectra at 465 nm were corrected using diffuse reflectance spectroscopy between 600 nm and 940 nm. The patented autoclavable Nitinol needle probe enables the acquisition of multispectral backscattering measurements of ultraviolet, visible, near-infrared and fluorescence spectra. As a phantom, a suspension of calcium carbonate (Calcilit) and water with physiological NADH concentrations between 0 mmol l-1 and 2.0 mmol l-1 were used to mimic human tissue. The light scattering characteristics were adjusted to match the backscattering attributes of human skin by modifying the concentration of Calcilit. To correct the scattering effects caused by the matrices of the samples, an algorithm based on the backscattered remission spectrum was employed to compensate the influence of multiscattering on the optical pathway through the dispersed phase. The monitored backscattered visible light was used to correct the fluorescence spectra and thereby to determine the true NADH concentrations at unknown Calcilit concentrations. Despite the simplicity of the presented algorithm, the root-mean-square error of prediction (RMSEP) was 0.093 mmol l-1.

A novel approach to regulate cell membrane permeability for ATP and NADH formation in Saccharomyces cerevisiae induced by air cold plasma

NASA Astrophysics Data System (ADS)

Dong, Xiaoyu; Liu, Tingting; Xiong, Yuqin

2017-02-01

Air cold plasma has been used as a novel method for enhancing microbial fermentation. The aim of this work was to explore the effect of plasma on membrane permeability and the formation of ATP and NADH in Saccharomyces cerevisiae, so as to provide valuable information for large-scale application of plasma in the fermentation industry. Suspensions of S. cerevisiae cells were exposed to air cold plasma for 0, 1, 2, 3, 4 and 5 min, and then subjected to various analyses prior to fermentation (0 h) and at the 9 and 21 h stages of fermentation. Compared with non-exposed cells, cells exposed to plasma for 1 min exhibited a marked increase in cytoplasmic free Ca2+ concentration as a result of the significant increase in membrane potential prior to fermentation. At the same time, the ATP level in the cell suspension decreased by about 40%, resulting in a reduction of about 60% in NADH prior to culturing. However, the levels of ATP and NADH in the culture at the 9 and 21 h fermentation stages were different from the level at 0 h. Taken together, the results indicated that exposure of S. cerevisiae to air cold plasma could increase its cytoplasmic free Ca2+ concentration by improving the cell membrane potential, consequently leading to changes in ATP and NADH levels. Supported by National Natural Science Foundation of China (Nos. 21246012, 21306015 and 21476032).

The Space Shuttle

NASA Technical Reports Server (NTRS)

Moffitt, William L.

2003-01-01

As missions have become increasingly more challenging over the years, the most adaptable and capable element of space shuttle operations has proven time and again to be human beings. Human space flight provides unique aspects of observation. interaction and intervention that can reduce risk and improve mission success. No other launch vehicle - in development or in operation today - can match the space shuttle's human space flight capabilities. Preserving U.S. leadership in human space flight requires a strategy to meet those challenges. The ongoing development of next generation vehicles, along with upgrades to the space shuttle, is the most effective means for assuring our access to space.

H2O2 Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase

PubMed Central

Hertzberger, Rosanne; Arents, Jos; Dekker, Henk L.; Pridmore, R. David; Gysler, Christof; Kleerebezem, Michiel

2014-01-01

Hydrogen peroxide production is a well-known trait of many bacterial species associated with the human body. In the presence of oxygen, the probiotic lactic acid bacterium Lactobacillus johnsonii NCC 533 excretes up to 1 mM H2O2, inducing growth stagnation and cell death. Disruption of genes commonly assumed to be involved in H2O2 production (e.g., pyruvate oxidase, NADH oxidase, and lactate oxidase) did not affect this. Here we describe the purification of a novel NADH-dependent flavin reductase encoded by two highly similar genes (LJ_0548 and LJ_0549) that are conserved in lactobacilli belonging to the Lactobacillus acidophilus group. The genes are predicted to encode two 20-kDa proteins containing flavin mononucleotide (FMN) reductase conserved domains. Reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin and is specific for NADH and not NADPH. The Km for FMN is 30 ± 8 μM, in accordance with its proposed in vivo role in H2O2 production. Deletion of the encoding genes in L. johnsonii led to a 40-fold reduction of hydrogen peroxide formation. H2O2 production in this mutant could only be restored by in trans complementation of both genes. Our work identifies a novel, conserved NADH-dependent flavin reductase that is prominently involved in H2O2 production in L. johnsonii. PMID:24487531

Shuttle mission simulator requirements report, volume 1, revision C

NASA Technical Reports Server (NTRS)

Burke, J. F.

1973-01-01

The contractor tasks required to produce a shuttle mission simulator for training crew members and ground personnel are discussed. The tasks will consist of the design, development, production, installation, checkout, and field support of a simulator with two separate crew stations. The tasks include the following: (1) review of spacecraft changes and incorporation of appropriate changes in simulator hardware and software design, and (2) the generation of documentation of design, configuration management, and training used by maintenance and instructor personnel after acceptance for each of the crew stations.

TERSSE. Definition of the total earth resources system for the shuttle era. Volume 9: Earth resources shuttle applications

NASA Technical Reports Server (NTRS)

Alverado, U.

1975-01-01

The use of the space shuttle for the Earth Resources Program is discussed. Several problems with respect to payload selection, integration, and mission planning were studied. Each of four shuttle roles in the sortie mode were examined and projected into an integrated shuttle program. Several representative Earth Resources missions were designed which would use the shuttle sortie as a platform and collectively include the four shuttle roles. An integrated flight program based on these missions was then developed for the first two years of shuttle flights. A set of broad implications concerning the uses of the shuttle for Earth Resources studies resulted.

Space Shuttle Projects

NASA Image and Video Library

1985-04-01

In this photograph the SYNCOM IV-3, also known as LEASAT 3, satellite moves away from the Space Shuttle Orbiter Discovery. SYNCOM (Hughes Geosynchronous Communication Satellite) provides communication services from geosynchronous orbit, principally to the U.S. Government. The satellite was launched on April 12, 1985, aboard the Space Shuttle Orbiter Discovery.

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

Space shuttle Enterprise is held aloft by a yellow sling and a set of cranes after it was removed from the top of NASA's 747 Shuttle Carrier Aircraft early Sunday morning at John F. Kennedy (JFK) International Airport in New York, Sunday, May 13, 2012 .The 747 was towed backwards so that Enterprise could be lowered. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to the Intrepid Sea, Air & Space Museum in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

NASA and United Space Alliance workers lower a yellow sling onto space shuttle Enterprise, which sits atop NASA's 747 Shuttle Carrier Aircraft (SCA) prior to it being demated a few hours later at John F. Kennedy (JFK) International Airport in New York, Saturday, May 12, 2012. Once the sling was firmly attached early Sunday morning, Enterprise was lifted from the SCA. The shuttle will be placed on a barge that will move by tugboat up the Hudson River to Intrepid in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Launch of the Shuttle Atlantis and begining of STS 61-B mission

NASA Image and Video Library

1985-11-26

61B-S-067 (26 Nov 1985) --- Space Shuttle Atlantis ascends into the night sky at 7:29 p.m. (EST), November 26, with a seven member crew and three communications satellites aboard. The STS 61-B crewmembers are Brewster Shaw Jr., Bryan D. O?Connor, Mary L. Cleave, Sherwood C. Spring, Jerry L. Ross and Payload Specialists Rodolfo Neri of Mexico (Morelos) and Charles D. Walker of McDonnell Douglas.

Achieving Space Shuttle ATO Using the Five-Segment Booster (FSB)

NASA Technical Reports Server (NTRS)

Sauvageau, Donald R.; McCool, Alex (Technical Monitor)

2001-01-01

As part of the continuing effort to identify approaches to improve the safety and reliability of the Space Shuttle system, a Five-Segment Booster (FSB) design was conceptualized as a replacement for the current Space Shuttle boosters. The FSB offers a simple, unique approach to improve astronaut safety and increase performance margin. To determine the feasibility of the FSB, a Phase A study effort was sponsored by NASA and directed by the Marshall Space Flight Center. This study was initiated in March of 1999 and completed in December of 2000. The basic objective of this study was to assess the feasibility of the FSB design concept and also estimate the cost and scope of a full-scale development program for the FSB. In order to ensure an effective and thorough evaluation of the FSB concept, four team members were put on contract to support various areas of importance in assessing the overall feasibility of the design approach.

NASA space shuttle lightweight seat

NASA Technical Reports Server (NTRS)

Hansen, Chris; Jermstad, Wayne; Lewis, James; Colangelo, Todd

1996-01-01

The Space Shuttle Lightweight Seat-Mission Specialist (LWS-MS) is a crew seat for the mission specialists who fly aboard the Space Shuttle. The LWS-MS is a lightweight replacement for the mission specialist seats currently flown on the Shuttle. Using state-of-the-art analysis techniques, a team of NASA and Lockheed engineers from the Johnson Space Center (JSC) designed a seat that met the most stringent requirements demanded of the new seats by the Shuttle program, and reduced the weight of the seats by 52%.

Shuttle - Mir Program Insignia

NASA Image and Video Library

1994-09-20

The rising sun signifies the dawn of a new era of human Spaceflight, the first phase of the United States/Russian space partnership, Shuttle-Mir. Mir is shown in its proposed final on orbit configuration. The Shuttle is shown in a generic tunnel/Spacehab configuration. The Shuttle/Mir combination, docked to acknowledge the union of the two space programs, orbits over an Earth devoid of any definable features or political borders to emphasize Earth as the home planet for all humanity. The individual stars near the Space Shuttle and the Russian Mir Space Station represent the previous individual accomplishments of Russia's space program and that of the United States. The binary star is a tribute to the previous United States-Russian joint human Spaceflight program, the Apollo-Soyuz Test Project (ASTP). The flags of the two nations are symbolized by flowing ribbons of the national colors interwoven in space to represent the two nations joint exploration of space. NASA SHUTTLE and PKA MNP are shown in the stylized logo fonts of the two agencies that are conducting this program.

Shuttle interaction study extension

NASA Technical Reports Server (NTRS)

1982-01-01

The following areas of Space Shuttle technology were discussed: variable altitude strategy, spacecraft servicing, propellant storage, orbiter plume impingement, space based design, mating (docking and berthing), shuttle fleet utilization, and mission/traffic model.

Shuttle Risk Progression by Flight

NASA Technical Reports Server (NTRS)

Hamlin, Teri; Kahn, Joe; Thigpen, Eric; Zhu, Tony; Lo, Yohon

2011-01-01

Understanding the early mission risk and progression of risk as a vehicle gains insights through flight is important: . a) To the Shuttle Program to understand the impact of re-designs and operational changes on risk. . b) To new programs to understand reliability growth and first flight risk. . Estimation of Shuttle Risk Progression by flight: . a) Uses Shuttle Probabilistic Risk Assessment (SPRA) and current knowledge to calculate early vehicle risk. . b) Shows impact of major Shuttle upgrades. . c) Can be used to understand first flight risk for new programs.

Stennis certifies final shuttle engine

NASA Image and Video Library

2008-10-22

Steam blasts out of the A-2 Test Stand at Stennis Space Center on Oct. 22 as engineers begin a certification test on engine 2061, the last space shuttle main flight engine scheduled to be built. Since 1975, Stennis has tested every space shuttle main engine used in the program - about 50 engines in all. Those engines have powered more than 120 shuttle missions - and no mission has failed as a result of engine malfunction. For the remainder of 2008 and throughout 2009, Stennis will continue testing of various space shuttle main engine components.

Space Shuttle Project

NASA Image and Video Library

1978-04-21

This is an interior ground level view of the Shuttle Orbiter Enterprise being lowered for mating to External Tank (ET) inside Marshall Space Flight Center's Dynamic Test Stand for Mated Vertical Ground Vibration tests (MVGVT). The tests marked the first time ever that the entire shuttle complement (including Orbiter, external tank, and solid rocket boosters) were mated vertically.

Expedition Six crew member Nikolai Budarin at pad before launch

NASA Technical Reports Server (NTRS)

2002-01-01

KENNEDY SPACE CENTER, FLA. - Expedition Six crew member Nikolai Budarin, of the Russian Space Agency, pauses in front of Space Shuttle Endeavour at Launch Pad 39A during a tour of Kennedy Space Center prior to his launch. The primary mission of STS-113 is bringing the Expedition 6 crew to the Station and returning the Expedition 5 crew to Earth. Another major objective of the mission is delivery of the Port 1 (P1) Integrated Truss Assembly, which will be attached to the port side of the S0 truss. Three spacewalks are planned to install and activate the truss and its associated equipment. Launch of Space Shuttle Endeavour on mission STS-113 is scheduled for Nov. 11 between midnight and 4 a.m. EST.

Space Shuttle

NASA Technical Reports Server (NTRS)

1975-01-01

A general description of the space shuttle program is presented, with emphasis on its application to the use of space for commercial, scientific, and defense needs. The following aspects of the program are discussed: description of the flight system (orbiter, external tank, solid rocket boosters) and mission profile, direct benefits related to life on earth (both present and expected), description of the space shuttle vehicle and its associated supporting systems, economic impacts (including indirect benefits such as lower inflation rates), listing of participating organizations.

Views supporting the Window Experiment (WINDEX) of shuttle environment

NASA Image and Video Library

1995-08-03

STS070-386-027 (13-22 JULY 1995) --- High-speed film provided this close-up view of the Space Shuttle Discovery’s aft, featuring the ignition of one of the primary thrusters. Note the impact of the firing on the starboard side of the vertical stabilizer. Crew members told a August 11, 1995, gathering of Johnson Space Center (JSC) employees that the Window Experiment (WINDEX) paid close attention to surface glow, jet plumes, water dumps, aurora and airglow. The data collection is part of an effort to avoid misinterpretation of measurements of Earth, the solar system and starts taken from satellites in low Earth-orbits and prevent damage to sensitive systems and solar arrays during rendezvous and docking. Such firings of the thrusters increase local densities of gases in the atmosphere dramatically and introduce non-natural elements that react with the atmosphere dramatically and spacecraft systems enveloped by the thruster plume. WINDEX recorded phenomena associated with thruster start-up and shut-down transients and observed the effect of the transients on Shuttle glow phenomenon.

STS-86 crew member Wolf dons a gas mask during TCDT

NASA Technical Reports Server (NTRS)

1997-01-01

STS-86 Mission Specialist David A. Wolf dons a gas mask as part of training exercises during the Terminal Countdown Demonstration Test (TCDT), a dress rehearsal for launch. Wolf is wearing the patch from his first and only mission to date, STS-58 in 1993. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. During the docking, Wolf will transfer to the orbiting Russian station and become a member of the Mir 24 crew, replacing U.S. astronaut C. Michael Foale, who has been on the Mir since the last docking mission, STS-84, in May. Launch of Mission STS-86 aboard the Space Shuttle Atlantis is targeted for Sept. 25.

Space Shuttle STS-1 SRB damage investigation

NASA Technical Reports Server (NTRS)

Nevins, C. D.

1982-01-01

The physical damage incurred by the solid rocket boosters during reentry on the initial space shuttle flight raised the question of whether the hardware, as designed, would yield the low cost per flight desired. The damage was quantified, the cause determined and specific design changes recommended which would preclude recurrence. Flight data, postflight analyses, and laboratory hardware examinations were used. The resultant findings pointed to two principal causes: failure of the aft skirt thermal curtain at the onset of reentry aerodynamic heating, and overloading of the aft shirt stiffening rings during water impact. Design changes were recommended on both the thermal curtain and the aft skirt structural members to prevent similar damage on future missions.

Localization and Function of the Membrane-bound Riboflavin in the Na+-translocating NADH:Quinone Oxidoreductase (Na+-NQR) from Vibrio cholerae*

PubMed Central

Casutt, Marco S.; Huber, Tamara; Brunisholz, René; Tao, Minli; Fritz, Günter; Steuber, Julia

2010-01-01

The sodium ion-translocating NADH:quinone oxidoreductase (Na+-NQR) from the human pathogen Vibrio cholerae is a respiratory membrane protein complex that couples the oxidation of NADH to the transport of Na+ across the bacterial membrane. The Na+-NQR comprises the six subunits NqrABCDEF, but the stoichiometry and arrangement of these subunits are unknown. Redox-active cofactors are FAD and a 2Fe-2S cluster on NqrF, covalently attached FMNs on NqrB and NqrC, and riboflavin and ubiquinone-8 with unknown localization in the complex. By analyzing the cofactor content and NADH oxidation activity of subcomplexes of the Na+-NQR lacking individual subunits, the riboflavin cofactor was unequivocally assigned to the membrane-bound NqrB subunit. Quantitative analysis of the N-terminal amino acids of the holo-complex revealed that NqrB is present in a single copy in the holo-complex. It is concluded that the hydrophobic NqrB harbors one riboflavin in addition to its covalently attached FMN. The catalytic role of two flavins in subunit NqrB during the reduction of ubiquinone to ubiquinol by the Na+-NQR is discussed. PMID:20558724

First Shuttle/747 Captive Flight

NASA Technical Reports Server (NTRS)

1977-01-01

The Space Shuttle prototype Enterprise rides smoothly atop NASA's first Shuttle Carrier Aircraft (SCA), NASA 905, during the first of the shuttle program's Approach and Landing Tests (ALT) at the Dryden Flight Research Center, Edwards, California, in 1977. During the nearly one year-long series of tests, Enterprise was taken aloft on the SCA to study the aerodynamics of the mated vehicles and, in a series of five free flights, tested the glide and landing characteristics of the orbiter prototype. In this photo, the main engine area on the aft end of Enterprise is covered with a tail cone to reduce aerodynamic drag that affects the horizontal tail of the SCA, on which tip fins have been installed to increase stability when the aircraft carries an orbiter. The Space Shuttle Approach and Landings Tests (ALT) program allowed pilots and engineers to learn how the Space Shuttle and the modified Boeing 747 Shuttle Carrier Aircraft (SCA) handled during low-speed flight and landing. The Enterprise, a prototype of the Space Shuttles, and the SCA were flown to conduct the approach and landing tests at the NASA Dryden Flight Research Center, Edwards, California, from February to October 1977. The first flight of the program consisted of the Space Shuttle Enterprise attached to the Shuttle Carrier Aircraft. These flights were to determine how well the two vehicles flew together. Five 'captive-inactive' flights were flown during this first phase in which there was no crew in the Enterprise. The next series of captive flights was flown with a flight crew of two on board the prototype Space Shuttle. Only three such flights proved necessary. This led to the free-flight test series. The free-flight phase of the ALT program allowed pilots and engineers to learn how the Space Shuttle handled in low-speed flight and landing attitudes. For these landings, the Enterprise was flown by a crew of two after it was released from the top of the SCA. The vehicle was released at altitudes

The Kinetic Reaction Mechanism of the Vibrio cholerae Sodium-dependent NADH Dehydrogenase*♦

PubMed Central

Tuz, Karina; Mezic, Katherine G.; Xu, Tianhao; Barquera, Blanca; Juárez, Oscar

2015-01-01

The sodium-dependent NADH dehydrogenase (Na+-NQR) is the main ion transporter in Vibrio cholerae. Its activity is linked to the operation of the respiratory chain and is essential for the development of the pathogenic phenotype. Previous studies have described different aspects of the enzyme, including the electron transfer pathways, sodium pumping structures, cofactor and subunit composition, among others. However, the mechanism of the enzyme remains to be completely elucidated. In this work, we have studied the kinetic mechanism of Na+-NQR with the use of steady state kinetics and stopped flow analysis. Na+-NQR follows a hexa-uni ping-pong mechanism, in which NADH acts as the first substrate, reacts with the enzyme, and the oxidized NAD leaves the catalytic site. In this conformation, the enzyme is able to capture two sodium ions and transport them to the external side of the membrane. In the last step, ubiquinone is bound and reduced, and ubiquinol is released. Our data also demonstrate that the catalytic cycle involves two redox states, the three- and five-electron reduced forms. A model that gathers all available information is proposed to explain the kinetic mechanism of Na+-NQR. This model provides a background to understand the current structural and functional information. PMID:26004776

Space Shuttle Project

NASA Image and Video Library

1992-07-09

As the orbiter Columbia (STS-50) rolled down Runway 33 of Kennedy Space Center's (KSC) Shuttle Landing Facility, its distinctively colored drag chute deployed to slow down the spaceship. This landing marked OV-102's first end-of-mission landing at KSC and the tenth in the program, and the second shuttle landing with the drag chute. Edwards Air Force Base, CA, was the designated prime for the landing of Mission STS-50, but poor weather necessitated the switch to KSC after a one-day extension of the historic flight. STS-50 was the longest in Shuttle program historyo date, lasting 13 days, 19 hours, 30 minutes and 4 seconds. A crew of seven and the USML-1 were aboard.

Food packages for Space Shuttle

NASA Technical Reports Server (NTRS)

Fohey, M. F.; Sauer, R. L.; Westover, J. B.; Rockafeller, E. F.

1978-01-01

The paper reviews food packaging techniques used in space flight missions and describes the system developed for the Space Shuttle. Attention is directed to bite-size food cubes used in Gemini, Gemini rehydratable food packages, Apollo spoon-bowl rehydratable packages, thermostabilized flex pouch for Apollo, tear-top commercial food cans used in Skylab, polyethylene beverage containers, Skylab rehydratable food package, Space Shuttle food package configuration, duck-bill septum rehydration device, and a drinking/dispensing nozzle for Space Shuttle liquids. Constraints and testing of packaging is considered, a comparison of food package materials is presented, and typical Shuttle foods and beverages are listed.

URF6, Last Unidentified Reading Frame of Human mtDNA, Codes for an NADH Dehydrogenase Subunit

NASA Astrophysics Data System (ADS)

Chomyn, Anne; Cleeter, Michael W. J.; Ragan, C. Ian; Riley, Marcia; Doolittle, Russell F.; Attardi, Giuseppe

1986-10-01

The polypeptide encoded in URF6, the last unassigned reading frame of human mitochondrial DNA, has been identified with antibodies to peptides predicted from the DNA sequence. Antibodies prepared against highly purified respiratory chain NADH dehydrogenase from beef heart or against the cytoplasmically synthesized 49-kilodalton iron-sulfur subunit isolated from this enzyme complex, when added to a deoxycholate or a Triton X-100 mitochondrial lysate of HeLa cells, specifically precipitated the URF6 product together with the six other URF products previously identified as subunits of NADH dehydrogenase. These results strongly point to the URF6 product as being another subunit of this enzyme complex. Thus, almost 60% of the protein coding capacity of mammalian mitochondrial DNA is utilized for the assembly of the first enzyme complex of the respiratory chain. The absence of such information in yeast mitochondrial DNA dramatizes the variability in gene content of different mitochondrial genomes.

Space Shuttle Abort Evolution

NASA Technical Reports Server (NTRS)

Henderson, Edward M.; Nguyen, Tri X.

2011-01-01

This paper documents some of the evolutionary steps in developing a rigorous Space Shuttle launch abort capability. The paper addresses the abort strategy during the design and development and how it evolved during Shuttle flight operations. The Space Shuttle Program made numerous adjustments in both the flight hardware and software as the knowledge of the actual flight environment grew. When failures occurred, corrections and improvements were made to avoid a reoccurrence and to provide added capability for crew survival. Finally some lessons learned are summarized for future human launch vehicle designers to consider.

Shuttle Discovery Fly-Over

NASA Image and Video Library

2012-04-17

Space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) flies over the Steven F. Udvar-Hazy Center, Tuesday, April 17, 2012, in Washington. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Dane Penland)

Space Shuttle Discovery Landing

NASA Image and Video Library

2012-04-17

Space Shuttle Discovery mounted atop a 747 Shuttle Carrier Aircraft (SCA) approaches the runway for landing at Washington Dulles International Airport, Tuesday April 17, 2012, in Sterling, Va. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Paul E. Alers)

Space Shuttle Discovery Landing

NASA Image and Video Library

2012-04-17

Space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) lands at Washington Dulles International Airport, Tuesday, April 17, 2012, in Sterling, Va. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Eric Long)

Shuttle Entry Imaging Using Infrared Thermography

NASA Technical Reports Server (NTRS)

Horvath, Thomas; Berry, Scott; Alter, Stephen; Blanchard, Robert; Schwartz, Richard; Ross, Martin; Tack, Steve

2007-01-01

During the Columbia Accident Investigation, imaging teams supporting debris shedding analysis were hampered by poor entry image quality and the general lack of information on optical signatures associated with a nominal Shuttle entry. After the accident, recommendations were made to NASA management to develop and maintain a state-of-the-art imagery database for Shuttle engineering performance assessments and to improve entry imaging capability to support anomaly and contingency analysis during a mission. As a result, the Space Shuttle Program sponsored an observation campaign to qualitatively characterize a nominal Shuttle entry over the widest possible Mach number range. The initial objectives focused on an assessment of capability to identify/resolve debris liberated from the Shuttle during entry, characterization of potential anomalous events associated with RCS jet firings and unusual phenomenon associated with the plasma trail. The aeroheating technical community viewed the Space Shuttle Program sponsored activity as an opportunity to influence the observation objectives and incrementally demonstrate key elements of a quantitative spatially resolved temperature measurement capability over a series of flights. One long-term desire of the Shuttle engineering community is to calibrate boundary layer transition prediction methodologies that are presently part of the Shuttle damage assessment process using flight data provided by a controlled Shuttle flight experiment. Quantitative global imaging may offer a complementary method of data collection to more traditional methods such as surface thermocouples. This paper reviews the process used by the engineering community to influence data collection methods and analysis of global infrared images of the Shuttle obtained during hypersonic entry. Emphasis is placed upon airborne imaging assets sponsored by the Shuttle program during Return to Flight. Visual and IR entry imagery were obtained with available airborne

Shuttle Discovery Mated to 747 SCA

NASA Technical Reports Server (NTRS)

1983-01-01

The Space Shuttle Discovery rides atop '905,' NASA's 747 Shuttle Carrier Aircraft, on its delivery flight from California to the Kennedy Space Center, Florida, where it was prepared for its first orbital mission for 30 August to 5 September 1984. The NASA 747, obtained in 1974, has special support struts atop the fuselage and internal strengthening to accommodate the additional weight of the orbiters. Small vertical fins have also been added to the tips of the horizontal stabilizers for additional stability due to air turbulence on the control surfaces caused by the orbiters. A second modified 747, no. 911, went in to service in November 1990 and is also used to ferry orbiters to destinations where ground transportation is not practical. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the

ENDEAVOR LANDS AT THE SHUTTLE LANDING FACILITY [SLF] ATOP 747 SHUTTLE CARRIER

NASA Technical Reports Server (NTRS)

1991-01-01

The newest addition to the space shuttle orbiter fleet, Endeavour, makes a graceful entrance at KSC atop the 747 Shuttle Carrier aircraft. Next stops for OV-105 are demating from the Boeing Aircraft and extended stays in the VAB and OPF. Endeavour's maiden space flight is scheduled in 1992.

Space Shuttle Payload Information Source

NASA Technical Reports Server (NTRS)

Griswold, Tom

2000-01-01

The Space Shuttle Payload Information Source Compact Disk (CD) is a joint NASA and USA project to introduce Space Shuttle capabilities, payload services and accommodations, and the payload integration process. The CD will be given to new payload customers or to organizations outside of NASA considering using the Space Shuttle as a launch vehicle. The information is high-level in a visually attractive format with a voice over. The format is in a presentation style plus 360 degree views, videos, and animation. Hyperlinks are provided to connect to the Internet for updates and more detailed information on how payloads are integrated into the Space Shuttle.

Spectral characteristics of Shuttle glow

NASA Technical Reports Server (NTRS)

Viereck, R. A.; Mende, S. B.; Murad, E.; Swenson, G. R.; Pike, C. P.; Culbertson, F. L.; Springer, R. C.

1992-01-01

The glowing cloud near the ram surfaces of the Space Shuttle was observed with a hand-held, intensified spectrograph operated by the astronauts from the aft-flight-deck of the Space Shuttle. The spectral measurements were made between 400 and 800 nm with a resolution of 3 nm. Analysis of the spectral response of the instrument and the transmission of the Shuttle window was performed on orbit using earth-airglow OH Meinel bands. This analysis resulted in a correction of the Shuttle glow intensity in the spectral region between 700 and 800 nm. The data presented in this report is in better agreement with laboratory measurements of the NO2 continuum.

TDRSS S-shuttle unique receiver equipment

NASA Astrophysics Data System (ADS)

Weinberg, A.; Schwartz, J. J.; Spearing, R.

1985-01-01

Beginning with STS-9, the Tracking and Date Relay Satellite system (TDRSS) will start providing S- and Ku-band communications and tracking support to the Space Shuttle and its payloads. The most significant element of this support takes place at the TDRSS White Sands Ground Terminal, which processes the Shuttle return link S- and Ku-band signals. While Ku-band hardware available to other TDRSS users is also applied to Ku-Shuttle, stringent S-Shuttle link margins have precluded the application of the standard TDRSS S-band processing equipment to S-Shuttle. It was therfore found necessary to develop a unique S-Shuttle Receiver that embodies state-of-the-art digital technology and processing techniques. This receiver, developed by Motorola, Inc., enhances link margins by 1.5 dB relative to the standard S-band equipment and its bit error rate performance is within a few tenths of a dB of theory. An overview description of the Space Shuttle Receiver Equipment (SSRE) is presented which includes the presentation of block diagrams and salient design features. Selected, measured performance results are also presented.

Space Shuttle Projects

NASA Image and Video Library

1978-09-01

This photograph shows stacking of the left side of the solid rocket booster (SRB) segments in the Dynamic Test Stand at the east test area of the Marshall Space Flight Center (MSFC). Staging shown here are the aft skirt, aft segment, and aft center segment. The SRB was attached to the external tank (ET) and then the orbiter later for the Mated Vertical Ground Vibration Test (MVGVT), that resumed in October 1978. The stacking of a complete Shuttle in the Dynamic Test Stand allowed test engineers to perform ground vibration testing on the Shuttle in its liftoff configuration. The purpose of the MVGVT is to verify that the Space Shuttle would perform as predicted during launch. The platforms inside the Dynamic Test Stand were modified to accommodate two SRB's to which the ET was attached.

Space Shuttle Projects

NASA Image and Video Library

1978-09-01

This photograph shows the left side of the solid rocket booster (SRB) segment as it awaits being mated to the nose cone and forward skirt in the Dynamic Test Stand at the east test area of the Marshall Space Flight Center (MSFC). The SRB would be attached to the external tank (ET) and then the orbiter later for the Mated Vertical Ground Vibration Test (MVGVT), that resumed in October 1978. The stacking of a complete Shuttle in the Dynamic Test Stand allowed test engineers to perform ground vibration testing on the Shuttle in its liftoff configuration. The purpose of the MVGVT was to verify that the Space Shuttle would perform as predicted during launch. The platforms inside the Dynamic Test Stand were modified to accommodate two SRB's to which the ET was attached.

Space Shuttle Projects

NASA Image and Video Library

1978-09-01

Workmen in the Dynamic Test Stand lowered the nose cone into place to complete stacking of the left side of the solid rocket booster (SRB) in the Dynamic Test Stand at the east test area of the Marshall Space Flight Center (MSFC). The SRB would be attached to the external tank (ET) and then the orbiter later for the Mated Vertical Ground Vibration Test (MVGVT), that resumed in October 1978. The stacking of a complete Shuttle in the Dynamic Test Stand allowed test engineers to perform ground vibration testing on the Shuttle in its liftoff configuration. The purpose of the MVGVT was to verify that the Space Shuttle would perform as predicted during launch. The platforms inside the Dynamic Test Stand were modified to accommodate two SRB'S to which the ET was attached.

Shuttle Carrier Aircraft (SCA) Fleet Photo

NASA Technical Reports Server (NTRS)

1995-01-01

NASA's two Boeing 747 Shuttle Carrier Aircraft (SCA) are seen here nose to nose at Dryden Flight Research Center, Edwards, California. The front mounting attachment for the Shuttle can just be seen on top of each. The SCAs are used to ferry Space Shuttle orbiters from landing sites back to the launch complex at the Kennedy Space Center, and also to and from other locations too distant for the orbiters to be delivered by ground transportation. The orbiters are placed atop the SCAs by Mate-Demate Devices, large gantry-like structures which hoist the orbiters off the ground for post-flight servicing, and then mate them with the SCAs for ferry flights. Features which distinguish the two SCAs from standard 747 jetliners are; three struts, with associated interior structural strengthening, protruding from the top of the fuselage (two aft, one forward) on which the orbiter is attached, and two additional vertical stabilizers, one on each end of the standard horizontal stabilizer, to enhance directional stability. The two SCAs are under the operational control of NASA's Johnson Space Center, Houston, Texas. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back

Space Shuttle redesign status

NASA Technical Reports Server (NTRS)

Brand, Vance D.

1986-01-01

NASA has conducted an extensive redesign effort for the Space Shutle in the aftermath of the STS 51-L Challenger accident, encompassing not only Shuttle vehicle and booster design but also such system-wide factors as organizational structure, management procedures, flight safety, flight operations, sustainable flight rate, and maintenance safeguards. Attention is presently given to Solid Rocket Booster redesign features, the Shuttle Main Engine's redesigned high pressure fuel and oxidizer turbopumps, the Shuttle Orbiter's braking and rollout (landing gear) system, the entry control mode of the flight control system, a 'split-S' abort maneuver for the Orbiter, and crew escape capsule proposals.

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

A set of cranes and wind restraints constructed to remove space shuttle Enterprise from atop NASA's 747 Shuttle Carrier Aircraft are being put into place at John F. Kennedy (JFK) International Airport in New York, Saturday, May 12, 2012. Enterprise will be placed on a barge that will move by tugboat up the Hudson River to Intrepid in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Discovery Landing

NASA Image and Video Library

2012-04-17

Space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) taxis in front of the main terminal at Washington Dulles International Airport, Tuesday, April 17, 2012, in Sterling, Va. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Eric Long)

MSFC shuttle lightning research

NASA Technical Reports Server (NTRS)

Vaughan, Otha H., Jr.

1993-01-01

The shuttle mesoscale lightning experiment (MLE), flown on earlier shuttle flights, and most recently flown on the following space transportation systems (STS's), STS-31, -32, -35, -37, -38, -40, -41, and -48, has continued to focus on obtaining additional quantitative measurements of lightning characteristics and to create a data base for use in demonstrating observation simulations for future spaceborne lightning mapping systems. These flights are also providing design criteria data for the design of a proposed shuttle MLE-type lightning research instrument called mesoscale lightning observational sensors (MELOS), which are currently under development here at MSFC.

Multi-Tasking: First Shuttle Mission Since Columbia Combines Test Flight, Catch-Up ISS Supply and Maintenance

NASA Technical Reports Server (NTRS)

Morring, Frank, Jr.

2005-01-01

NASA's space shuttle fleet is nearing its return to flight with a complex mission on board Discovery that will combine tests of new hardware and procedures adopted in the wake of Columbia's loss with urgent repairs and resupply for the International Space Station. A seven-member astronaut crew has trained throughout most of the two-year hiatus in shuttle operations for the 13-day mission, shooting for a three-week launch window that opens May 15. The window, and much else about the STS-114 mission, is constrained by NASA's need to ensure it has fixed the ascent/debris problem that doomed Columbia and its crew as they attempted to reenter the atmosphere on Feb. 1, 2003. The window was selected so Discovery's ascent can be photographed in daylight with 107 different ground- and aircraft-based cameras to monitor the redesigned external tank for debris shedding. Fixed cameras and the shuttle crew will also photograph the tank in space after it has been jettisoned.

Achromobacter denitrificans Strain YD35 Pyruvate Dehydrogenase Controls NADH Production To Allow Tolerance to Extremely High Nitrite Levels

PubMed Central

Doi, Yuki; Shimizu, Motoyuki; Fujita, Tomoya; Nakamura, Akira; Takizawa, Noboru

2014-01-01

We identified the extremely nitrite-tolerant bacterium Achromobacter denitrificans YD35 that can grow in complex medium containing 100 mM nitrite (NO2−) under aerobic conditions. Nitrite induced global proteomic changes and upregulated tricarboxylate (TCA) cycle enzymes as well as antioxidant proteins in YD35. Transposon mutagenesis generated NO2−-hypersensitive mutants of YD35 that had mutations at genes for aconitate hydratase and α-ketoglutarate dehydrogenase in the TCA cycle and a pyruvate dehydrogenase (Pdh) E1 component, indicating the importance of TCA cycle metabolism to NO2− tolerance. A mutant in which the pdh gene cluster was disrupted (Δpdh mutant) could not grow in the presence of 100 mM NO2−. Nitrite decreased the cellular NADH/NAD+ ratio and the cellular ATP level. These defects were more severe in the Δpdh mutant, indicating that Pdh contributes to upregulating cellular NADH and ATP and NO2−-tolerant growth. Exogenous acetate, which generates acetyl coenzyme A and then is metabolized by the TCA cycle, compensated for these defects caused by disruption of the pdh gene cluster and those caused by NO2−. These findings demonstrate a link between NO2− tolerance and pyruvate/acetate metabolism through the TCA cycle. The TCA cycle mechanism in YD35 enhances NADH production, and we consider that this contributes to a novel NO2−-tolerating mechanism in this strain. PMID:24413603

Space Shuttle Projects

NASA Image and Video Library

1976-01-01

This is a cutaway illustration of the Space Shuttle external tank (ET) with callouts. The giant cylinder, higher than a 15-story building, with a length of 154-feet (47-meters) and a diameter of 27.5-feet (8.4-meters), is the largest single piece of the Space Shuttle. During launch, the ET also acts as a backbone for the orbiter and solid rocket boosters. Separate pressurized tank sections within the external tank hold the liquid hydrogen fuel and liquid oxygen oxidizer for the Shuttle's three main engines. During launch, the ET feeds the fuel under pressure through 17-inch (43.2-centimeter) ducts that branch off into smaller lines that feed directly into the main engines. The main engines consume 64,000 gallons (242,260 liters) of fuel each minute. Machined from aluminum alloys, the Space Shuttle's external tank is currently the only part of the launch vehicle that is not reused. After its 526,000-gallons (1,991,071 liters) of propellants are consumed during the first 8.5-minutes of flight, it is jettisoned from the orbiter and breaks up in the upper atmosphere, its pieces falling into remote ocean waters. The Marshall Space Flight Center was responsible for developing the ET.

Shuttle: forever young?

PubMed

Sietzen, Frank

2002-01-01

NASA has started a 4-phase program of upgrades designed to increase safety and extend use of the space shuttles through the year 2020. Phase I is aimed at improving vehicle safety and supporting the space station. Phase II is aimed at combating obsolescence and includes a checkout launch and control system and protection from micrometeoroids and orbital debris. Phase III is designed to expand or enhance the capabilities of the shuttle and includes development of an auxiliary power unit, avionics, a channel-wall nozzle, extended nose landing gear, long-life fuel cells, a nontoxic orbital maneuvering system/reaction control system, and a water membrane evaporator. Phase IV is aimed at design of system changes that would alter the shuttle mold line and configuration; projects include a five-segment solid rocket booster, liquid flyback boosters, and a crew escape module.

Artificial Neural Network Test Support Development for the Space Shuttle PRCS Thrusters

NASA Technical Reports Server (NTRS)

Lehr, Mark E.

2005-01-01

A significant anomaly, Fuel Valve Pilot Seal Extrusion, is affecting the Shuttle Primary Reaction Control System (PRCS) Thrusters, and has caused 79 to fail. To help address this problem, a Shuttle PRCS Thruster Process Evaluation Team (TPET) was formed. The White Sands Test Facility (WSTF) and Boeing members of the TPET have identified many discrete valve current trace characteristics that are predictive of the problem. However, these are difficult and time consuming to identify and trend by manual analysis. Based on this exhaustive analysis over months, 22 thrusters previously delivered by the Depot were identified as high risk for flight failures. Although these had only recently been installed, they had to be removed from Shuttles OV103 and OV104 for reprocessing, by directive of the Shuttle Project Office. The resulting impact of the thruster removal, replacement, and valve replacement was significant (months of work and hundreds of thousands of dollars). Much of this could have been saved had the proposed Neural Network (NN) tool described in this paper been in place. In addition to the significant benefits to the Shuttle indicated above, the development and implementation of this type of testing will be the genesis for potential Quality improvements across many areas of WSTF test data analysis and will be shared with other NASA centers. Future tests can be designed to incorporate engineering experience via Artificial Neural Nets (ANN) into depot level acceptance of hardware. Additionally, results were shared with a NASA Engineering and Safety Center (NESC) Super Problem Response Team (SPRT). There was extensive interest voiced among many different personnel from several centers. There are potential spin-offs of this effort that can be directly applied to other data acquisition systems as well as vehicle health management for current and future flight vehicles.

Space Shuttle Projects

NASA Image and Video Library

1999-08-01

Designed by the crew members, the STS-103 emblem depicts the Space Shuttle Discovery approaching the Hubble Space Telescope (HST) prior to its capture and berthing. The purpose of the mission was to remove and replace some of the Telescope's older and out-of-date systems with newer, more reliable and more capable ones, and to make repairs to HST's exterior thermal insulation that had been damaged by more than nine years of exposure to the space environment. The horizontal and vertical lines centered on the Telescope symbolize the ability to reach and maintain a desired attitude in space, essential to the instrument's scientific operation. The preservation of this ability was one of the primary objectives of the mission. After the flight, the Telescope resumed its successful exploration of deep space and will continue to be used to study solar system objects, stars in the making, late phases of stellar evolution, galaxies and the early history of the universe. HST, as represented on this emblem was inspired by views from previous servicing missions, with its solar arrays illuminated by the Sun, providing a striking contrast with the blackness of space and the night side of Earth.

Space Shuttle Projects

NASA Image and Video Library

2001-01-01

This illustration is an orbiter cutaway view with callouts. The orbiter is both the brains and heart of the Space Transportation System (STS). About the same size and weight as a DC-9 aircraft, the orbiter contains the pressurized crew compartment (which can normally carry up to seven crew members), the huge cargo bay, and the three main engines mounted on its aft end. There are three levels to the crew cabin. Uppermost is the flight deck where the commander and the pilot control the mission. The middeck is where the gallery, toilet, sleep stations, and storage and experiment lockers are found for the basic needs of weightless daily living. Also located in the middeck is the airlock hatch into the cargo bay and space beyond. It is through this hatch and airlock that astronauts go to don their spacesuits and marned maneuvering units in preparation for extravehicular activities, more popularly known as spacewalks. The Space Shuttle's cargo bay is adaptable to hundreds of tasks. Large enough to accommodate a tour bus (60 x 15 feet or 18.3 x 4.6 meters), the cargo bay carries satellites, spacecraft, and spacelab scientific laboratories to and from Earth orbit. It is also a work station for astronauts to repair satellites, a foundation from which to erect space structures, and a hold for retrieved satellites to be returned to Earth. Thermal tile insulation and blankets (also known as the thermal protection system or TPS) cover the underbelly, bottom of the wings, and other heat-bearing surfaces of the orbiter to protect it during its fiery reentry into the Earth's atmosphere. The Shuttle's 24,000 individual tiles are made primarily of pure-sand silicate fibers, mixed with a ceramic binder. The solid rocket boosters (SRB's) are designed as an in-house Marshall Space Flight Center project, with United Space Boosters as the assembly and refurbishment contractor. The solid rocket motor (SRM) is provided by the Morton Thiokol Corporation.

Space Shuttle Projects

NASA Image and Video Library

1992-09-01

The STS-53 crew portrait included astronauts (front left to right): Guion S. Bluford, and James S. Voss, mission specialists. On the back row, left to right, are David M. Walker, commander; Robert D. Cabana, Pilot; and Michael R. (Rick) Clifford, mission specialist. The crew launched aboard the Space Shuttle Discovery on December 2, 1992 at 8:24:00 am (EST). This mission marked the final classified shuttle flight for the Department of Defense (DOD).

Engineering a synthetic anaerobic respiration for reduction of xylose to xylitol using NADH output of glucose catabolism by Escherichia coli AI21.

PubMed

Iverson, Andrew; Garza, Erin; Manow, Ryan; Wang, Jinhua; Gao, Yuanyuan; Grayburn, Scott; Zhou, Shengde

2016-04-16

Anaerobic rather than aerobic fermentation is preferred for conversion of biomass derived sugars to high value redox-neutral and reduced commodities. This will likely result in a higher yield of substrate to product conversion and decrease production cost since substrate often accounts for a significant portion of the overall cost. To this goal, metabolic pathway engineering has been used to optimize substrate carbon flow to target products. This approach works well for the production of redox neutral products such as lactic acid from redox neutral sugars using the reducing power NADH (nicotinamide adenine dinucleotide, reduced) generated from glycolysis (2 NADH per glucose equivalent). Nevertheless, greater than two NADH per glucose catabolized is needed for the production of reduced products (such as xylitol) from redox neutral sugars by anaerobic fermentation. The Escherichia coli strain AI05 (ΔfrdBC ΔldhA ΔackA Δ(focA-pflB) ΔadhE ΔptsG ΔpdhR::pflBp 6-(aceEF-lpd)), previously engineered for reduction of xylose to xylitol using reducing power (NADH equivalent) of glucose catabolism, was further engineered by 1) deleting xylAB operon (encoding for xylose isomerase and xylulokinase) to prevent xylose from entering the pentose phosphate pathway; 2) anaerobically expressing the sdhCDAB-sucABCD operon (encoding for succinate dehydrogenase, α-ketoglutarate dehydrogenase and succinyl-CoA synthetase) to enable an anaerobically functional tricarboxcylic acid cycle with a theoretical 10 NAD(P)H equivalent per glucose catabolized. These reducing equivalents can be oxidized by synthetic respiration via xylose reduction, producing xylitol. The resulting strain, AI21 (pAI02), achieved a 96 % xylose to xylitol conversion, with a yield of 6 xylitol per glucose catabolized (molar yield of xylitol per glucose consumed (YRPG) = 6). This represents a 33 % improvement in xylose to xylitol conversion, and a 63 % increase in xylitol yield per glucose catabolized over

Representative shuttle evaporative heat sink

NASA Technical Reports Server (NTRS)

Hixon, C. W.

1978-01-01

The design, fabrication, and testing of a representative shuttle evaporative heat sink (RSEHS) system which vaporizes an expendable fluid to provide cooling for the shuttle heat transport fluid loop is reported. The optimized RSEHS minimum weight design meets or exceeds the shuttle flash evaporator system requirements. A cold trap which cryo-pumps flash evaporator exhaust water from the CSD vacuum chamber test facility to prevent water contamination of the chamber pumping equipment is also described.

STS-88 crew members and technicians participate in their CEIT in the SSPF

NASA Technical Reports Server (NTRS)

1997-01-01

Pilot Rick Sturckow and Mission Specialist Jerry Ross, both members of the STS-88 crew, participate with technicians in the Crew Equipment Interface Test for that mission in KSC's Space Station Processing Facility. STS-88, the first International Space Station assembly flight, is targeted for launch in July 1998 aboard Space Shuttle Endeavour.

Perfect launch for Space Shuttle Discovery on mission STS-105

NASA Technical Reports Server (NTRS)

2001-01-01

KENNEDY SPACE CENTER, Fla. -- Smoke billows out from Launch Pad 39A as Space Shuttle Discovery soars into the blue sky on mission STS-105 to the International Space Station. Liftoff occurred at 5:10:14 p.m. EDT on this second launch attempt. Launch countdown activities for the 12-day mission were called off Aug. 9 during the T-9 minute hold due to the high potential for lightning, a thick cloud cover and the potential for showers. Besides the Shuttle crew of four, Discovery carries the Expedition Three crew who will replace Expedition Two on the International Space Station. The mission includes the third flight of an Italian-built Multi-Purpose Logistics Module delivering additional scientific racks, equipment and supplies for the Space Station, and two spacewalks. Part of the payload is the Early Ammonia Servicer (EAS) tank, which will be attached to the Station during the spacewalks. The EAS contains spare ammonia for the Station'''s cooling system. The three-member Expedition Two crew will be returning to Earth aboard Discovery after a five-month stay on the Station.

STS-86 crew members Wolf, Chretien and Titov in M-113

NASA Technical Reports Server (NTRS)

1997-01-01

STS-86 crew members get a ride in, and learn to operate, an M-113 armored personnel carrier as part of training exercises during the Terminal Countdown Demonstration Test (TCDT), a dress rehearsal for launch. George Hoggard, in back at left, a training officer with KSC Fire Services, provides this part of the training to Mission Specialists Wendy B. Lawrence, to the right of Hoggard; Vladimir Georgievich Titov of the Russian Space Agency; and Scott E. Parazynski. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. During the docking, Titov and Parazynski are scheduled to conduct a spacewalk primarily to retrieve four suitcase-sized environmental payloads from the exterior of the Mir docking module. Also during the mission, STS-86 Mission Specialist David A. Wolf will transfer to the orbiting Russian station and become a member of the Mir 24 crew, replacing U.S. astronaut C. Michael Foale, who has been on the Mir since the last docking mission, STS-84, in May. Launch of Mission STS-86 aboard the Space Shuttle Atlantis is targeted for Sept. 25 from Launch Pad 39A.

STS-93 crew members take part in an emergency egress exercise

NASA Technical Reports Server (NTRS)

1999-01-01

During an emergency egress exercise at the launch pad, Pilot Jeffrey S. Ashby (left) and Commander Eileen M. Collins (right) hurry down the yellow-painted path to a slidewire basket. The baskets are part of an emergency escape route for persons in the Shuttle and on the Rotating Service Structure. The STS-93 crew members have been taking part in Terminal Countdown Demonstration Test (TCDT) activities that include the emergency exit training and a launch-day dress rehearsal culminating with a simulated main engine cut-off. Other crew members participating are Mission Specialists Catherine G. Coleman (Ph.D.), Steven A. Hawley (Ph.D.), and Michel Tognini of France, who represents the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as a Shuttle commander. The primary mission of STS-93 is the release of the Chandra X-ray Observatory, which will allow scientists from around the world to obtain unprecedented X-ray images of exotic environments in space to help understand the structure and evolution of the universe. The targeted launch date for STS-93 is no earlier than July 20 at 12:36 a.m. EDT from Launch Pad 39B.

Chemical Shuttle Additives in Lithium Ion Batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Patterson, Mary

2013-03-31

The goals of this program were to discover and implement a redox shuttle that is compatible with large format lithium ion cells utilizing LiNi{sub 1/3}Mn{sub 1/3}Co{sub 1/3}O{sub 2} (NMC) cathode material and to understand the mechanism of redox shuttle action. Many redox shuttles, both commercially available and experimental, were tested and much fundamental information regarding the mechanism of redox shuttle action was discovered. In particular, studies surrounding the mechanism of the reduction of the oxidized redox shuttle at the carbon anode surface were particularly revealing. The initial redox shuttle candidate, namely 2-(pentafluorophenyl)-tetrafluoro-1,3,2-benzodioxaborole (BDB) supplied by Argonne National Laboratory (ANL, Lemont,more » Illinois), did not effectively protect cells containing NMC cathodes from overcharge. The ANL-RS2 redox shuttle molecule, namely 1,4-bis(2-methoxyethoxy)-2,5-di-tert-butyl-benzene, which is a derivative of the commercially successful redox shuttle 2,5-di-tert-butyl-1,4-dimethoxybenzene (DDB, 3M, St. Paul, Minnesota), is an effective redox shuttle for cells employing LiFePO{sub 4} (LFP) cathode material. The main advantage of ANL-RS2 over DDB is its larger solubility in electrolyte; however, ANL-RS2 is not as stable as DDB. This shuttle also may be effectively used to rebalance cells in strings that utilize LFP cathodes. The shuttle is compatible with both LTO and graphite anode materials although the cell with graphite degrades faster than the cell with LTO, possibly because of a reaction with the SEI layer. The degradation products of redox shuttle ANL-RS2 were positively identified. Commercially available redox shuttles Li{sub 2}B{sub 12}F{sub 12} (Air Products, Allentown, Pennsylvania and Showa Denko, Japan) and DDB were evaluated and were found to be stable and effective redox shuttles at low C-rates. The Li{sub 2}B{sub 12}F{sub 12} is suitable for lithium ion cells utilizing a high voltage cathode (potential that

Space Shuttle Enterprise Demate

NASA Image and Video Library

2012-05-12

The space shuttle Enterprise, mounted on transport vehicle, is backed into a temporary hanger after being demated from the NASA 747 Shuttle Carrier Aircraft (SCA) at John F. Kennedy (JFK) International Airport in Jamica, New York, Sunday, May 13, 2012. Enterprise will be placed on a barge that will move by tugboat up the Hudson River to the Intrepid Sea, Air & Space Museum in June. The shuttle will be lifted by crane and placed on the flight deck of the Intrepid, where it will be on exhibit to the public starting this summer in a temporary climate-controlled pavilion. Photo Credit: (NASA/Kim Shiflet)

Space Shuttle Discovery Landing

NASA Image and Video Library

2012-04-17

Space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) lands at Washington Dulles International Airport, Tuesday, April 17, 2012, in Sterling, Va. The Steven F. Udvar-Hazy Center is seen in the background. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Eric Long)

Nuclear Shuttle Logistics Configuration

NASA Technical Reports Server (NTRS)

1971-01-01

This 1971 artist's concept shows the Nuclear Shuttle in both its lunar logistics configuraton and geosynchronous station configuration. As envisioned by Marshall Space Flight Center Program Development persornel, the Nuclear Shuttle would deliver payloads to lunar orbits or other destinations then return to Earth orbit for refueling and additional missions.

Fluorescence lifetime analysis and effect of magnesium ions on binding of NADH to human aldehyde dehydrogenase 1

USDA-ARS?s Scientific Manuscript database

Aldehyde dehydrogenase 1 (ALDH1) catalyzes oxidation of toxic aldehydes to carboxylic acids. Physiologic levels of Mg2+ ions influence ALDH1 activity in part by increasing NADH binding affinity to the enzyme thus reducing activity. By using time-resolved fluorescence spectroscopy, we have resolved t...

Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brosey, Chris A.; Ho, Chris; Long, Winnie Z.

Apoptosis-inducing factor (AIF) is critical for mitochondrial respiratory complex biogenesis and for mediating necroptotic parthanatos; these functions are seemingly regulated by enigmatic allosteric switching driven by NADH charge-transfer complex (CTC) formation. In this paper, we define molecular pathways linking AIF's active site to allosteric switching regions by characterizing dimer-permissive mutants using small-angle X-ray scattering (SAXS) and crystallography and by probing AIF-CTC communication networks using molecular dynamics simulations. Collective results identify two pathways propagating allostery from the CTC active site: (1) active-site H454 links to S480 of AIF's central β-strand to modulate a hydrophobic border at the dimerization interface, and (2)more » an interaction network links AIF's FAD cofactor, central β-strand, and Cβ-clasp whereby R529 reorientation initiates C-loop release during CTC formation. Finally, this knowledge of AIF allostery and its flavoswitch mechanism provides a foundation for biologically understanding and biomedically controlling its participation in mitochondrial homeostasis and cell death.« less

Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor

DOE PAGES

Brosey, Chris A.; Ho, Chris; Long, Winnie Z.; ...

2016-11-03

Apoptosis-inducing factor (AIF) is critical for mitochondrial respiratory complex biogenesis and for mediating necroptotic parthanatos; these functions are seemingly regulated by enigmatic allosteric switching driven by NADH charge-transfer complex (CTC) formation. In this paper, we define molecular pathways linking AIF's active site to allosteric switching regions by characterizing dimer-permissive mutants using small-angle X-ray scattering (SAXS) and crystallography and by probing AIF-CTC communication networks using molecular dynamics simulations. Collective results identify two pathways propagating allostery from the CTC active site: (1) active-site H454 links to S480 of AIF's central β-strand to modulate a hydrophobic border at the dimerization interface, and (2)more » an interaction network links AIF's FAD cofactor, central β-strand, and Cβ-clasp whereby R529 reorientation initiates C-loop release during CTC formation. Finally, this knowledge of AIF allostery and its flavoswitch mechanism provides a foundation for biologically understanding and biomedically controlling its participation in mitochondrial homeostasis and cell death.« less

Space Shuttle operational logistics plan

NASA Technical Reports Server (NTRS)

Botts, J. W.

1983-01-01

The Kennedy Space Center plan for logistics to support Space Shuttle Operations and to establish the related policies, requirements, and responsibilities are described. The Directorate of Shuttle Management and Operations logistics responsibilities required by the Kennedy Organizational Manual, and the self-sufficiency contracting concept are implemented. The Space Shuttle Program Level 1 and Level 2 logistics policies and requirements applicable to KSC that are presented in HQ NASA and Johnson Space Center directives are also implemented.

Space Shuttle Project

NASA Image and Video Library

1993-04-17

A four-million-mile journey draws to a flawless ending as the orbiter Discovery (STS-56) lands at Kennedy Space Center's (KSC) Shuttle Landing Facility. Aboard for the second shuttle mission of 1993 were a crew of five and the Atmospheric Laboratory for Applications and Science 2 (ATLAS 2), the second in a series of missions to study the sun's energy output and Earth's middle atmosphere chemical make-up, and how these factors affect levels of ozone.

Space Shuttle Project

NASA Image and Video Library

1992-09-12

A smooth countdown culminated in a picture-perfect launch as the Space Shuttle Endeavour (STS-47) climbed skyward atop a ladder of billowing smoke. Primary payload for the plarned seven-day flight was Spacelab-J science laboratory. The second flight of Endeavour marks a number of historic firsts: the first space flight of an African-American woman, the first Japanese citizen to fly on a Space Shuttle, and the first married couple to fly in space.

Space Shuttle Discovery Launch

NASA Image and Video Library

2008-05-31

NASA Administrator, Michael Griffin watches the launch of the Space Shuttle Discovery (STS-124) from the Launch Control Center Saturday, May 31, 2008, at the Kennedy Space Center in Cape Canaveral, Fla. The Shuttle lifted off from launch pad 39A at 5:02 p.m. EDT. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Projects

NASA Image and Video Library

1991-08-01

The free-flying Tracking and Data Relay Satellite-E (TDRS-E), still attached to an Inertial Upper Stage (IUS), was photographed by one of the crewmembers during the STS-43 mission. The TDRS-E was boosted by the IUS into geosynchronous orbit and positioned to remain stationary 22,400 miles above the Pacific Ocean southwest of Hawaii. The TDRS system provides almost uninterrupted communications with Earth-orbiting Shuttles and satellites, and had replaced the intermittent coverage provided by globe-encircling ground tracking stations used during the early space program. The TDRS can transmit and receive data, and track a user spacecraft in a low Earth orbit. The IUS is an unmarned transportation system designed to ferry payloads from low Earth orbit to higher orbits that are unattainable by the Shuttle. The Space Shuttle Orbiter Atlantis for the STS-43 mission was launched on August 2, 1991.

Space Shuttle Projects

NASA Image and Video Library

1991-08-01

The primary payload of the STS-43 mission, Tracking and Data Relay Satellite-E (TDRS-E) attached to an Inertial Upper Stage (IUS) was photographed at the moment of its release from the cargo bay of the Space Shuttle Orbiter Atlantis. The TDRS-E was boosted by the IUS into geosynchronous orbit and positioned to remain stationary 22,400 miles above the Pacific Ocean southwest of Hawaii. The TDRS system provides almost uninterrupted communications with Earth-orbiting Shuttles and satellites, and had replaced the intermittent coverage provided by globe-encircling ground tracking stations used during the early space program. The TDRS can transmit and receive data, and track a user spacecraft in a low Earth orbit. The IUS is an unmarned transportation system designed to ferry payloads from low Earth orbit to higher orbits that are unattainable by the Shuttle. The launch of STS-43 occurred on August 2, 1991.

KENNEDY SPACE CENTER, FLA. - The rising sun and some scattered clouds provide a picturesque backdrop for the Space Shuttle Discovery as it travels along the crawlerway toward Launch Pad 39A in preparation for the STS-82 mission. The Shuttle is on a Mobile Launcher Platform, and the entire assemblage is being carried by a large, tracked vehicle called the crawler transporter. A seven-member crew will perform the second servicing of the orbiting Hubble Space Telescope (HST) during the 10-day STS-82 flight, whcih is targeted for a Feb. 11 liftoff.

NASA Image and Video Library

1997-01-17

KENNEDY SPACE CENTER, FLA. - The rising sun and some scattered clouds provide a picturesque backdrop for the Space Shuttle Discovery as it travels along the crawlerway toward Launch Pad 39A in preparation for the STS-82 mission. The Shuttle is on a Mobile Launcher Platform, and the entire assemblage is being carried by a large, tracked vehicle called the crawler transporter. A seven-member crew will perform the second servicing of the orbiting Hubble Space Telescope (HST) during the 10-day STS-82 flight, whcih is targeted for a Feb. 11 liftoff.

Malate valves: Old shuttles with new perspectives.

PubMed

Selinski, Jennifer; Scheibe, Renate

2018-06-22

Malate valves act as powerful systems for balancing the ATP/NAD(P)H ratio required in various subcellular compartments in plant cells. As components of malate valves, isoforms of malate dehydrogenases (MDHs) and dicarboxylate translocators catalyze the reversible interconversion of malate and oxaloacetate and their transport. Depending on the coenzyme specificity of the MDH isoforms, either NADH or NADPH can be transported indirectly. Arabidopsis thaliana possesses nine genes encoding MDH isoenzymes: Activities of NAD-dependent MDHs have been detected in mitochondria, peroxisomes, cytosol and plastids, respectively. In addition, chloroplasts possess a NADP-dependent MDH isoform. The NADP-MDH as part of the "light malate valve" plays an important role as a poising mechanism to adjust the ATP/NADPH ratio in the stroma. Its activity is strictly regulated by post-translational redox-modification mediated via the ferredoxin-thioredoxin system and fine control via the NADP + /NADP(H) ratio, thereby maintaining redox homeostasis under changing conditions. In contrast, the plastid NAD-MDH ("dark malate valve") is constitutively active and its lack leads to failure in early embryo development. While redox regulation of the main cytosolic MDH isoform has been shown, the knowledge about regulation of the other two cytosolic MDHs as well as NAD-MDH isoforms from peroxisomes and mitochondria is still lacking. Knockout mutants lacking the isoforms from chloroplasts, mitochondria, and peroxisomes have been characterized, but not much is known about cytosolic NAD-MDH isoforms and their role in planta. This review updates the current knowledge on MDH isoforms and the shuttle systems for intercompartmental dicarboxylate exchange focusing on the various metabolic functions of these valves. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Formation of vacuolar tannin deposits in the chlorophyllous organs of Tracheophyta: from shuttles to accretions.

PubMed

Brillouet, Jean-Marc; Romieu, Charles; Lartaud, Marc; Jublanc, Elodie; Torregrosa, Laurent; Cazevieille, Chantal

2014-11-01

Most Tracheophyta synthesize-condensed tannins (also called proanthocyanidins), polymers of catechins, which appear in the vacuole as uniformly stained deposits-termed tannin accretions-lining the inner face of the tonoplast. A large body of evidence argues that tannins are formed in recently described thylakoid-derived organelles, the tannosomes, which are packed in membrane-bound shuttles (Brillouet et al. 2013); it has been suggested that shuttles agglomerate into tannin accretions. The aim of the study was to describe the ontogenesis of tannin accretions in members of the Tracheophyta. For this purpose, fresh specimens of young tissues from diverse Tracheophyta were cut, gently lacerated in paraformaldehyde, and examined using light, epifluorescence, confocal, and transmission electron microscopy. Fresh samples were also incubated with gelatin-Oregon Green, a fluorescent marker of condensed tannins. Our observations showed that vacuolar accretions (1 → 40 μm), that constitute the typical form of tannin storage in tannin-producing Tracheophyta, are formed by agglomeration (not fusion) of shuttles containing various proportions of chlorophylls and tannins.

Site-directed mutagenesis of conserved cysteine residues in NqrD and NqrE subunits of Na+-translocating NADH:quinone oxidoreductase.

PubMed

Fadeeva, M S; Bertsova, Y V; Verkhovsky, M I; Bogachev, A V

2008-02-01

Each of two hydrophobic subunits of Na+-translocating NADH:quinone oxidoreductase (NQR), NqrD and NqrE, contain a pair of strictly conserved cysteine residues within their transmembrane alpha-helices. Site-directed mutagenesis showed that substitutions of these residues in NQR of Vibrio harveyi blocked the Na+-dependent and 2-n-heptyl-4-hydroxyquinoline N-oxide-sensitive quinone reductase activity of the enzyme. However, these mutations did not affect the interaction of NQR with NADH and menadione. It was demonstrated that these conserved cysteine residues are necessary for the correct folding and/or the stability of the NQR complex. Mass and EPR spectroscopy showed that NQR from V. harveyi bears only a 2Fe-2S cluster as a metal-containing prosthetic group.

Space Shuttle Projects

NASA Image and Video Library

1983-07-01

This photograph was taken during the final assembly phase of the Space Shuttle light weight external tanks (LWT) 5, 6, and 7 at the Michoud Assembly Facility in New Orleans, Louisiana. The giant cylinder, higher than a 15-story building, with a length of 154-feet (47-meters) and a diameter of 27.5-feet (8.4-meters), is the largest single piece of the Space Shuttle. During launch, the external tank (ET) acts as a backbone for the orbiter and solid rocket boosters. In separate, internal pressurized tank sections, the ET holds the liquid hydrogen fuel and liquid oxygen oxidizer for the Shuttle's three main engines. During launch, the ET feeds the fuel under pressure through 17-inch (43.2-centimeter) ducts which branch off into smaller lines that feed directly into the main engines. Some 64,000 gallons (242,260 liters) of fuel are consumed by the main engines each minute. Machined from aluminum alloys, the Space Shuttle's ET is the only part of the launch vehicle that currently is not reused. After its 526,000 gallons (1,991,071 liters) of propellants are consumed during the first 8.5 minutes of flight, it is jettisoned from the orbiter and breaks up in the upper atmosphere, its pieces falling into remote ocean waters. The Marshall Space Flight Center was responsible for developing the ET

Space Shuttle Projects

NASA Image and Video Library

1989-03-01

This STS-29 mission onboard photo depicts the External Tank (ET) falling toward the ocean after separation from the Shuttle orbiter Discovery. The giant cylinder, higher than a 15-story building, with a length of 154-feet (47-meters) and a diameter of 27,5-feet (8.4-meters), is the largest single piece of the Space Shuttle. During launch, the ET also acts as a backbone for the orbiter and solid rocket boosters. In separate, internal pressurized tank sections, the ET holds the liquid hydrogen fuel and liquid oxygen oxidizer for the Shuttle's three main engines. During launch, the ET feeds the fuel under pressure through 17-inch (43.2-centimeter) ducts which branch off into smaller lines that feed directly into the main engines. Some 64,000 gallons (242,260 liters) of fuel are consumed by the main engines each minute. Machined from aluminum alloys, the Space Shuttle's ET is the only part of the launch vehicle that currently is not reused. After its 526,000 gallons (1,991,071 liters) of propellants are consumed during the first 8.5 minutes of flight, it is jettisoned from the orbiter and breaks up in the upper atmosphere, its pieces falling into remote ocean waters. The Marshall Space Flight Center was responsible for developing the ET.

Space Shuttle Project

NASA Image and Video Library

1992-08-24

A crewmember aboard the Space Shuttle Orbiter Atlantis (STS-46) used a 70mm handheld camera to capture this medium closeup view of early operations with the Tethered Satellite System (TSS). TSS-1 is being deployed from its boom as it is perched above the cargo bay of the Earth-orbiting Shuttle circling the Earth at an altitude of 296 kilometers (184 miles), the TSS-1 will be well within the tenuous, electrically charged layer of the atmosphere known as the ionosphere. There, a satellite attached to the orbiter by a thin conducting cord, or tether, will be reeled from the Shuttle payload bay. On this mission the satellite was plarned to be deployed 20 kilometers (12.5 miles) above the Shuttle. The conducting tether will generate high voltage and electrical currents as it moves through the atmosphere allowing scientists to examine the electrodynamics of a conducting tether system. These studies will not only increase our understanding of physical processes in the near-Earth space environment, but will also help provide an explanation for events witnessed elsewhere in the solar system. The crew of the STS-46 mission were unable to reel the satellite as planned. After several unsuccessful attempts, they were only able to extend the satellite 9.8 kilometers (6.1 miles). The TSS was a cooperative development effort by the Italian Space Agency (ASI), and NASA.

Space Shuttle Placement Announcement

NASA Image and Video Library

2011-04-12

From left, Pilot of the first space shuttle mission, STS-1, Bob Crippen, NASA Administrator Charles Bolden, NASA Johnson Space Center Director of Flight Crew Operations, and Astronaut, Janet Kavandi, NASA Kennedy Space Center Director and former astronaut Bob Cabana, and Endeavour Vehicle Manager for United Space Alliance Mike Parrish pose for a photograph outside of the an Orbiter Processing Facility with the space shuttle Atlantis shortly after Bolden announced where four space shuttle orbiters will be permanently displayed at the conclusion of the Space Shuttle Program, Tuesday, April 12, 2011, at Kennedy Space Center in Cape Canaveral, Fla. The four orbiters, Enterprise, which currently is on display at the Smithsonian's Steven F. Udvar-Hazy Center near Washington Dulles International Airport, will move to the Intrepid Sea, Air & Space Museum in New York, Discovery will move to Udvar-Hazy, Endeavour will be displayed at the California Science Center in Los Angeles and Atlantis, in background, will be displayed at the Kennedy Space Center Visitor’s Complex. Photo Credit: (NASA/Bill Ingalls)

Space-Shuttle Emulator Software

NASA Technical Reports Server (NTRS)

Arnold, Scott; Askew, Bill; Barry, Matthew R.; Leigh, Agnes; Mermelstein, Scott; Owens, James; Payne, Dan; Pemble, Jim; Sollinger, John; Thompson, Hiram;

A mitochondrial NADH-dependent fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma brucei.

PubMed

Coustou, Virginie; Besteiro, Sébastien; Rivière, Loïc; Biran, Marc; Biteau, Nicolas; Franconi, Jean-Michel; Boshart, Michael; Baltz, Théo; Bringaud, Frédéric

2005-04-29

Trypanosoma brucei is a parasitic protist responsible for sleeping sickness in humans. The procyclic stage of T. brucei expresses a soluble NADH-dependent fumarate reductase (FRDg) in the peroxisome-like organelles called glycosomes. This enzyme is responsible for the production of about 70% of the excreted succinate, the major end product of glucose metabolism in this form of the parasite. Here we functionally characterize a new gene encoding FRD (FRDm1) expressed in the procyclic stage. FRDm1 is a mitochondrial protein, as evidenced by immunolocalization, fractionation of digitonin-permeabilized cells, and expression of EGFP-tagged FRDm1 in the parasite. RNA interference was used to deplete FRDm1, FRDg, or both together. The analysis of the resulting mutant cell lines showed that FRDm1 is responsible for 30% of the cellular NADH-FRD activity, which solves a long standing debate regarding the existence of a mitochondrial FRD in trypanosomatids. FRDg and FRDm1 together account for the total NADH-FRD activity in procyclics, because no activity was measured in the double mutant lacking expression of both proteins. Analysis of the end products of 13C-enriched glucose excreted by these mutant cell lines showed that FRDm1 contributes to the production of between 14 and 44% of the succinate excreted by the wild type cells. In addition, depletion of one or both FRD enzymes results in up to 2-fold reduction of the rate of glucose consumption. We propose that FRDm1 is involved in the maintenance of the redox balance in the mitochondrion, as proposed for the ancestral soluble FRD presumably present in primitive anaerobic cells.

Space Shuttle Discovery Landing

NASA Image and Video Library

2012-04-17

NASA Deputy Administrator Lori Garver, at podium, speaks to those in attendance at Apron W after the 747 Shuttle Carrier Aircraft (SCA) with space shuttle Discovery mounted on top rolled to a halt at Washington Dulles International Airport, Tuesday, April 17, 2012 in Sterling, Va. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Dane Penland)

Shuttle Imaging Radar - Geologic applications

NASA Technical Reports Server (NTRS)

Macdonald, H.; Bridges, L.; Waite, W.; Kaupp, V.

1982-01-01

The Space Shuttle, on its second flight (November 12, 1981), carried the first science and applications payload which provided an early demonstration of Shuttle's research capabilities. One of the experiments, the Shuttle Imaging Radar-A (SIR-A), had as a prime objective to evaluate the capability of spaceborne imaging radars as a tool for geologic exploration. The results of the experiment will help determine the value of using the combination of space radar and Landsat imagery for improved geologic analysis and mapping. Preliminary analysis of the Shuttle radar imagery with Seasat and Landsat imagery from similar areas provides evidence that spaceborne radars can significantly complement Landsat interpretation, and vastly improve geologic reconnaissance mapping in those areas of the world that are relatively unmapped because of perpetual cloud cover.

Restartable solid motor stage for shuttle applications

NASA Technical Reports Server (NTRS)

Rohrbaugh, D. J.

1973-01-01

The application of restartable solid motor stages to shuttle missions has been shown to provide a viable supplement to the shuttle program. Restartable solid motors in the 3000 pound class provide a small expendable transfer stage that reduces the demand on the shuttle for the lower energy missions. Shuttle operational requirements and preliminary performance data provided an input for defining design features required for restartable solid motor applications. These data provided a basis for a configuration definition that is compatible with shuttle operations. Mission by mission analysis showed the impact on a NASA supplied mission model. The results showed a 15% reduction in the number of shuttle flights required. In addition the amount of shuttle capability used to complete the mission objectives was significantly reduced. For example, in the 1979 missions there was a 62% reduction in shuttle capability used. The study also showed that the solid motor could provide a supplement to the TUG that would allow TUGS to be used in a recoverable rather than an expendable mode. The study shows a 71% reduction in the number of TUGs that would be expended.

Brazing of refractory, superalloy, and composite materials for Space Shuttle applications.

NASA Technical Reports Server (NTRS)

Beuyukian, C. S.

1972-01-01

Research work concerning the metallic portion of the shuttle-orbiter heat shield (expected to experience temperatures up to 2500 F) is described. The five metals being evaluated are TD-Ni-Cr, Cb-C129Y, Cb752, Hayes 188, and Inconel 625. Brazing techniques whereby pairs of these materials are joined into thin-membered assemblies for heat shield applications are described. Results obtained with the vacuum-furnace brazing process are examined. In addition, the use of brazed aluminum-boron metal-matrix-contoured composite structures for heat shield applications is evaluated.

Space Shuttle Projects

NASA Image and Video Library

1978-09-29

This photo depicts the installation of an External Tank (ET) into the Marshall Space Flight Center Dynamic Test Stand, building 4550. It is being mated to the Solid Rocket Boosters (SRB's) for a Mated Vertical Ground Vibration Test (MVGVT). At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and is the only part of the vehicle that is not reusable.

Space Shuttle Project

NASA Image and Video Library

1992-09-12

A smooth countdown culminated in a picture-perfect launch as the Space Shuttle Orbiter Endeavour (STS-47) climbed skyward atop a ladder of billowing smoke on September 12, 1992. The primary payload for the plarned seven-day flight was the Spacelab-J science laboratory. The second flight of Endeavour marks a number of historic firsts: the first space flight of an African-American woman, the first Japanese citizen to fly on a Space Shuttle, and the first married couple to fly in space.

Space Shuttle Projects

NASA Image and Video Library

1985-05-30

The crewmembers of Space Shuttle mission 51-F have chosen as their insignia this design by Houston artist Skip Bradley. The Space Shuttle Challenger is depicted ascending toward the heavens in search of new knowledge in the field of solar and steallar astronomy, with its Spacelab 2 payload. The constellations Leo and Orion are in the positions they will be in, relative to the sun during the flight. The nineteen stars signify that this will be the 19th STS flight.

Environmental protection requirements for scout/shuttle auxiliary stages

NASA Technical Reports Server (NTRS)

Qualls, G. L.; Kress, S. S.; Storey, W. W.; Ransdell, P. N.

1980-01-01

The requirements for enabling the Scout upper stages to endure the expected temperature, mechanical shock, acoustical and mechanical vibration environments during a specified shuttle mission were determined. The study consisted of: determining a shuttle mission trajectory for a 545 kilogram (1200 pound) Scout payload; compilation of shuttle environmental conditions; determining of Scout upper stages environments in shuttle missions; compilation of Scout upper stages environmental qualification criteria and comparison to shuttle mission expected environments; and recommendations for enabling Scout upper stages to endure the exptected shuttle mission environments.

Lactate shuttles in nature.

PubMed

Brooks, G A

2002-04-01

Once thought to be the consequence of oxygen lack in contracting skeletal muscle, the glycolytic product lactate is formed and utilized continuously under fully aerobic conditions. "Cell-cell" and "intracellular lactate shuttle" concepts describe the roles of lactate in the delivery of oxidative and gluconeogenic substrates, as well as in cell signalling. Examples of cell-cell shuttles include lactate exchanges between white-glycolytic and red-oxidative fibres within a working muscle bed, between working skeletal muscle and heart, and between tissues of net lactate release and gluconeogenesis. Lactate exchange between astrocytes and neurons that is linked to glutamatergic signalling in the brain is an example of a lactate shuttle supporting cell-cell signalling. Lactate uptake by mitochondria and pyruvate-lactate exchange in peroxisomes are examples of intracellular lactate shuttles. Lactate exchange between sites of production and removal is facilitated by monocarboxylate transport proteins, of which there are several isoforms, and, probably, also by scaffolding proteins. The mitochondrial lactate-pyruvate transporter appears to work in conjunction with mitochondrial lactate dehydrogenase, which permits lactate to be oxidized within actively respiring cells. Hence mitochondria function to establish the concentration and proton gradients necessary for cells with high mitochondrial densities (e.g. cardiocytes) to take up and oxidize lactate. Arteriovenous difference measurements on working cardiac and skeletal muscle beds as well as NMR spectral analyses of these tissues show that lactate is formed and oxidized within the cells of formation in vivo. Glycolysis and lactate oxidation within cells permits high flux rates and the maintenance of redox balance in the cytosol and mitochondria. Other examples of intracellular lactate shuttles include lactate uptake and oxidation in sperm mitochondria and the facilitation of beta-oxidation in peroxisomes by pyruvate

From it's position at 175 statute miles above Earth, the Space Shuttle Endeavour has encountered

NASA Technical Reports Server (NTRS)

1996-01-01

STS-77 ESC VIEW --- From it's position at 175 statute miles above Earth, the Space Shuttle Endeavour has encountered some colorful and attractive scenes heading into sunsets and sunrises. This particular encounter, captured with an Electronic Still Camera (ESC), occurred on flight day four, during which the six-member crew deployed the Passive Aerodynamically Stabilized Magnetically Damped Satellite (PAMS) - Satellite Test Unit (STU). GMT: 13:48:47.

NASA Contingency Shuttle Crew Support (CSCS) Medical Operations

NASA Technical Reports Server (NTRS)

Adams, Adrien

2010-01-01

The genesis of the space shuttle began in the 1930's when Eugene Sanger came up with the idea of a recyclable rocket plane that could carry a crew of people. The very first Shuttle to enter space was the Shuttle "Columbia" which launched on April 12 of 1981. Not only was "Columbia" the first Shuttle to be launched, but was also the first to utilize solid fuel rockets for U.S. manned flight. The primary objectives given to "Columbia" were to check out the overall Shuttle system, accomplish a safe ascent into orbit, and to return back to earth for a safe landing. Subsequent to its first flight Columbia flew 27 more missions but on February 1st, 2003 after a highly successful 16 day mission, the Columbia, STS-107 mission, ended in tragedy. With all Shuttle flight successes come failures such as the fatal in-flight accident of STS 107. As a result of the STS 107 accident, and other close-calls, the NASA Space Shuttle Program developed contingency procedures for a rescue mission by another Shuttle if an on-orbit repair was not possible. A rescue mission would be considered for a situation where a Shuttle and the crew were not in immediate danger, but, was unable to return to Earth or land safely. For Shuttle missions to the International Space Station (ISS), plans were developed so the Shuttle crew would remain on board ISS for an extended period of time until rescued by a "rescue" Shuttle. The damaged Shuttle would subsequently be de-orbited unmanned. During the period when the ISS Crew and Shuttle crew are on board simultaneously multiple issues would need to be worked including, but not limited to: crew diet, exercise, psychological support, workload, and ground contingency support

The Na+-Translocating NADH:Quinone Oxidoreductase Enhances Oxidative Stress in the Cytoplasm of Vibrio cholerae

PubMed Central

Muras, Valentin; Dogaru-Kinn, Paul; Minato, Yusuke; Häse, Claudia C.

2016-01-01

ABSTRACT We searched for a source of reactive oxygen species (ROS) in the cytoplasm of the human pathogen Vibrio cholerae and addressed the mechanism of ROS formation using the dye 2′,7′-dichlorofluorescein diacetate (DCFH-DA) in respiring cells. By comparing V. cholerae strains with or without active Na+-translocating NADH:quinone oxidoreductase (Na+-NQR), this respiratory sodium ion redox pump was identified as a producer of ROS in vivo. The amount of cytoplasmic ROS detected in V. cholerae cells producing variants of Na+-NQR correlated well with rates of superoxide formation by the corresponding membrane fractions. Membranes from wild-type V. cholerae showed increased superoxide production activity (9.8 ± 0.6 μmol superoxide min−1 mg−1 membrane protein) compared to membranes from the mutant lacking Na+-NQR (0.18 ± 0.01 μmol min−1 mg−1). Overexpression of plasmid-encoded Na+-NQR in the nqr deletion strain resulted in a drastic increase in the formation of superoxide (42.6 ± 2.8 μmol min−1 mg−1). By analyzing a variant of Na+-NQR devoid of quinone reduction activity, we identified the reduced flavin adenine dinucleotide (FAD) cofactor of cytoplasmic NqrF subunit as the site for intracellular superoxide formation in V. cholerae. The impact of superoxide formation by the Na+-NQR on the virulence of V. cholerae is discussed. IMPORTANCE In several studies, it was demonstrated that the Na+-NQR in V. cholerae affects virulence in a yet unknown manner. We identified the reduced FAD cofactor in the NADH-oxidizing NqrF subunit of the Na+-NQR as the site of superoxide formation in the cytoplasm of V. cholerae. Our study provides the framework to understand how reactive oxygen species formed during respiration could participate in the regulated expression of virulence factors during the transition from aerobic to microaerophilic (intestinal) habitats. This hypothesis may turn out to be right for many other pathogens which, like V. cholerae, depend on

Intrepid Space Shuttle Pavilion Opening

NASA Image and Video Library

2012-07-19

The space shuttle Enterprise is seen shortly after the grand opening of the Space Shuttle Pavilion at the Intrepid Sea, Air & Space Museum on Thursday, July 19, 2012 in New York. Photo Credit: (NASA/Bill Ingalls)

A PIECE OF THE SPACE SHUTTLE CHALLENGER WASHED ASHORE AT COCOA BEACH

NASA Technical Reports Server (NTRS)

1996-01-01

A Cocoa Beach front-end loader holds a large piece of debris from the Space Shuttle orbiter Challenger after it washed ashore in Cocoa Beach near the Coconuts on the Beach restaurant and bar. The piece, about 15 feet by 6 feet, is believed to be part of an elevon or rudder. It is one of the biggest pieces to wash ashore to date. A smaller piece was found several blocks south. NASA recovered thousands of pounds of debris from the Atlantic Ocean after the Jan. 28, 1986 accident which destroyed the Shuttle and claimed the lives of the seven crew members; about 50 percent of the orbiter remained in the ocean after search operations were suspended. Those remains are stored at Cape Canaveral Air Station, mostly in two Minutemen silos. The two newly recovered pieces will be brought by flatbed truck to KSC's Security Patrol Headquarters on Contractor Road for examination and temporary storage.

Optically measured NADH concentrations are unaffected by propofol induced EEG silence during transient cerebral hypoperfusion in anesthetized rabbits☆

PubMed Central

Wang, Mei; Agarwal, Sachin; Mayevsky, Avraham; Joshi, Shailendra

2014-01-01

The neuroprotective benefit of intra-operative anesthetics is widely described and routinely aimed to invoke electroencephalographic (EEG) silence in anticipation of transient cerebral ischemia. Previous rat survival studies have questioned an additional benefit from achieving EEG silence during transient global cerebral hypoperfusion. Surgical preparation on twelve New Zealand white rabbits under ketamine–propofol anesthesia, included placement of skull screws for bilateral EEG monitoring, skull shaving for laser Doppler probes, and a 5 mm diameter right temporal craniotomy for the NADH probe. Transient global cerebral hypoperfusion was achieved with bilateral internal carotid artery occlusion and pharmacologically induced systemic hypotension. All animals acted as controls, and had cerebral hypoperfusion under baseline propofol anesthesia with an active EEG. Thereafter, animals were randomized to receive bolus injection of intracarotid (3–5 mg) or intravenous (10–20 mg) 1% propofol to create EEG silence for 1–2 min. The data collected at baseline, peak hypoperfusion, and 5 and 10 min post hypoperfusion was analyzed by repeated measures ANOVA with post hoc Bonferroni–Dunn test. Eleven of the twelve rabbits completed the protocol. Hemodynamics and cerebral blood flow changes were comparable in all the animals. Compared to controls, the increase in NADH during ischemia was unaffected by EEG silence with either intravenous or intraarterial propofol. We failed to observe any significant additional attenuation of the elevation in NADH levels with propofol induced EEG silence during transient global cerebral hypoperfusion. This is consistent with previous rat survival studies showing that EEG silence was not required for full neuroprotective effects of pentothal anesthesia. PMID:21570061

Electromagnetic Compatibility for the Space Shuttle

NASA Technical Reports Server (NTRS)

Scully, Robert C.

2004-01-01

This slide presentation reviews the Space Shuttle electromagnetic compatibility (EMC). It includes an overview of the design of the shuttle with the areas that are of concern for the electromagnetic compatibility. It includes discussion of classical electromagnetic interference (EMI) and the work performed to control the electromagnetic interference. Another area of interest is electrostatic charging and the threat of electrostatic discharge and the attempts to reduce damage to the Shuttle from these possible hazards. The issue of electrical bonding is als reviewed. Lastly the presentation reviews the work performed to protect the shuttle from lightning, both in flight and on the ground.

Planned development of the space shuttle vehicle

NASA Technical Reports Server (NTRS)

1972-01-01

Information pertaining to the planned development of the space shuttle vehicle is presented. The package contains: (1) President's statement; (2) Dr. Fletcher's statement; (3) space shuttle fact sheet; (4) important reasons for the space shuttle.

Astronaut Stephen Oswald and fellow crew members on middeck

NASA Technical Reports Server (NTRS)

1995-01-01

Astronaut Stephen S. Oswald (center), STS-67 mission commander, is seen with two of his fellow crew members and an experiment which required a great deal of his time on the middeck of the Earth orbiting Space Shuttle Endeavour. Astronaut John M. Grunsfeld inputs mission data on a computer while listening to a cassette. Astronaut William G. Gregory (right edge of frame), pilot, consults a check list. The Middeck Active Control Experiment (MACE), not in use here, can be seen in upper center.

Space Shuttle Projects

NASA Image and Video Library

1996-03-01

The STS-78 patch links past with present to tell the story of its mission and science through a design imbued with the strength and vitality of the 2-dimensional art of North America's northwest coast Indians. Central to the design is the space Shuttle whose bold lines and curves evoke the Indian image for the eagle, a native American symbol of power and prestige as well as the national symbol of the United States. The wings of the Shuttle suggest the wings of the eagle whose feathers, indicative of peace and friendship in Indian tradition, are captured by the U forms, a characteristic feature of Northwest coast Indian art. The nose of the Shuttle is the strong downward curve of the eagle's beak, and the Shuttle's forward windows, the eagle's eyes, represented through the tapered S forms again typical of this Indian art form. The basic black and red atoms orbiting the mission number recall the original NASA emblem while beneath, utilizing Indian ovoid forms, the major mission scientific experiment package LMS (Life and Materials Sciences) housed in the Shuttle's cargo bay is depicted in a manner reminiscent of totem-pole art. This image of a bird poised for flight, so common to Indian art, is counterpointed by an equally familiar Tsimshian Indian symbol, a pulsating sun with long hyperbolic rays, the symbol of life. Within each of these rays are now encased crystals, the products of this mission's 3 major, high-temperature materials processing furnaces. And as the sky in Indian lore is a lovely open country, home of the Sun Chief and accessible to travelers through a hole in the western horizon, so too, space is a vast and beckoning landscape for explorers launched beyond the horizon. Beneath the Tsimshian sun, the colors of the earth limb are appropriately enclosed by a red border representing life to the Northwest coast Indians. The Indian colors of red, navy blue, white, and black pervade the STS-78 path. To the right of the Shuttle-eagle, the constellation

Fourth Report of the Task Force on the Shuttle-Mir Rendezvous and Docking Missions

NASA Technical Reports Server (NTRS)

1995-01-01

On December 6, 1994, the NASA Administrator, Mr. Daniel Goldin, requested that Lt. Gen. Thomas P. Stafford, in his role as the Chairman of the NASA Advisory Council Task Force on the Shuttle-Mir Rendezvous and Docking Missions, lead a team composed of several Task Force members and technical advisors' to Russia with the goal of reviewing preparations and readiness for the upcoming international Space Station Phase 1 missions. In his directions to Gen. Stafford, Mr. Goldin requested that the review team focus its initial efforts on safety of flight issues for the following Phase 1A missions: the Soyuz TM-21 mission which will carry U.S. astronaut Dr. Norman Thagard and cosmonauts Lt. Col. Vladimir Dezhurov and Mr. Gennady Strekalov aboard a Soyuz spacecraft to the Mir Station; the Mir 18 Main Expedition during which Thagard and his fellow cosmonauts, Dezhurov and Strokalov, will spend approximately three months aboard the Mir Station; the STS-71 Space Shuttle mission which will perform the first Shuttle-Mir docking, carry cosmonauts Col. Anatoly SoloViev and Mr. Nikolai Budarin to the Mir Station, and return Thagard, Dezhurov, and Strekalov to Earth.

The shuttle main engine: A first look

NASA Technical Reports Server (NTRS)

Schreur, Barbara

1996-01-01

Anyone entering the Space Shuttle Main Engine (SSME) team attends a two week course to become familiar with the design and workings of the engine. This course provides intensive coverage of the individual hardware items and their functions. Some individuals, particularly those involved with software maintenance and development, have felt overwhelmed by this volume of material and their lack of a logical framework in which to place it. To provide this logical framework, it was decided that a brief self-taught introduction to the overall operation of the SSME should be designed. To aid the people or new team members with an interest in the software, this new course should also explain the structure and functioning of the controller and its software. This paper presents a description of this presentation.

Space Shuttle Discovery Fly-By

NASA Image and Video Library

2012-04-17

Space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) flies over the Steven F. Udvar-Hazy Center, Tuesday, April 17, 2012, in Washington. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Smithsonian Institution/Eric Long)

EA Shuttle Document Retention Effort

NASA Technical Reports Server (NTRS)

Wagner, Howard A.

2010-01-01

This slide presentation reviews the effort of code EA at Johnson Space Center (JSC) to identify and acquire databases and documents from the space shuttle program that are adjudged important for retention after the retirement of the space shuttle.

Space Shuttle Placement Announcement

NASA Image and Video Library

2011-04-12

Pilot of the first space shuttle mission, STS-1, Bob Crippen speaks at an event where NASA Administrator Charles Bolden announced where four space shuttle orbiters will be permanently displayed at the conclusion of the Space Shuttle Program, Tuesday, April 12, 2011, at Kennedy Space Center in Cape Canaveral, Fla. The four orbiters, Enterprise, which currently is on display at the Smithsonian's Steven F. Udvar-Hazy Center near Washington Dulles International Airport, will move to the Intrepid Sea, Air & Space Museum in New York, Discovery will move to Udvar-Hazy, Endeavour will be displayed at the California Science Center in Los Angeles and Atlantis, in background, will be displayed at the Kennedy Space Center Visitor’s Complex. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Placement Announcement

NASA Image and Video Library

2011-04-12

The space shuttle Atlantis is seen in the Orbiter Processing Facility at an event where NASA Administrator Charles Bolden announced where four space shuttle orbiters will be permanently displayed at the conclusion of the Space Shuttle Program, Tuesday, April 12, 2011, at Kennedy Space Center in Cape Canaveral, Fla. The four orbiters, Enterprise, which currently is on display at the Smithsonian's Steven F. Udvar-Hazy Center near Washington Dulles International Airport, will move to the Intrepid Sea, Air & Space Museum in New York, Discovery will move to Udvar-Hazy, Endeavour will be displayed at the California Science Center in Los Angeles and Atlantis, will be displayed at the Kennedy Space Center Visitor’s Complex. Photo Credit: (NASA/Bill Ingalls)

Space Shuttle Placement Announcement

NASA Image and Video Library

2011-04-12

Pilot of the first space shuttle mission, STS-1, Bob Crippen speaks at an event where NASA Administrator Charles Bolden announced where four space shuttle orbiters will be permanently displayed at the conclusion of the Space Shuttle Program, Tuesday, April 12, 2011, at Kennedy Space Center in Cape Canaveral, Fla. The four orbiters, Enterprise, which currently is on display at the Smithsonian's Steven F. Udvar-Hazy Center near Washington Dulles International Airport, will move to the Intrepid Sea, Air & Space Museum in New York, Discovery will move to Udvar-Hazy, Endeavour will be displayed at the California Science Center in Los Angeles and Atlantis, in background, will be displayed at the Kennedy Space Center Visitor’s Complex. Photo Credit: (NASA/Bill Ingalls)

Real-time evaluation of tissue vitality by monitoring of microcirculatory blood flow, HbO2, and mitochondrial NADH redox state

NASA Astrophysics Data System (ADS)

Deutsch, Assaf; Pevzner, Eliyahu; Jaronkin, Alex; Mayevsky, Avraham

2004-06-01

Monitoring of tissue vitality (oxygen supply/demand) in real time is very rare in clinical practice although its use as an early warning alarming system, for clinical care medicine, is very practical. In our previous communication (SPIE 2003) we described the Tissue Spectroscope - TiSpec02, by which tissue microcirculatory blood flow (TBF) and mitochondrial NADH fluorescence were measured using a single light source (390nm). In order to improve the measurement capabilities as well as to decrease dramatically the size and cost of this clinical device, we have changed the TiSpec02 into a multi-wavelength illumination system in the new TiSpec03. In order to measure microcirculatory blood flow by laser Doppler flowmetry we used a 785nm laser diode. For mitochondrial NADH fluorescence measurement we adopted the 370nm LED. For the determination of the oxygenation level of hemoglobin (HbO2) we used the 2-wavelength reflectance technique. This new monitored parameter that was added to the TiSpec03 increases the accuracy of the diagnosis of tissue vitality. The bundle of optical fibers used to connect the tissue to the TiSpec03, was integrated into a special anchoring methodology depending on the monitored tissue or organ. In order to test the performance of the improved TiSpec we have used it in experimental animals brain models exposed to various pathophysiological conditions. Rats and gerbils were anesthetized and the fiber optic probe was located epidurally used dental acrylic cement. During anoxia and ischemia the lack of O2 led to a clear decrease in TBF and HbO2 while NADH shows a large elevation. When brain activation was induced by cortical spreading depression (SD), the elevated O2 consumption was recorded as a large oxidation (decrease) of mitochondrial NADH while TBF increase dramatically. Blood HbO2 was not affected significantly by the SD wave.

Experiment definition phase shuttle laboratory (LDRL-10.6 experiment): Shuttle sortie to elliptical orbit satellite

NASA Technical Reports Server (NTRS)

Goodwin, F. E.; Nussmeier, T. A.; Stokes, L. S.; Vourgourakis, E. J.

1976-01-01

The following topics were reviewed: (1) design options for shuttle terminal, (2) elliptical orbit satellite design options, (3) shuttle terminal details, (4) technology status and development requirements, (5) transmitter technology, and (6) carbon dioxide laser life studies.

STS-134 crew and Expedition 24/25 crew member Shannon Walker

NASA Image and Video Library

2010-03-25

JSC2010-E-043667 (25 March 2010) --- NASA astronaut Mark Kelly, STS-134 commander, uses the virtual reality lab in the Space Vehicle Mock-up Facility at NASA's Johnson Space Center to train for some of his duties aboard the space shuttle and space station. This type of computer interface, paired with virtual reality training hardware and software, helps to prepare crew members for dealing with space station elements.

Intrepid Space Shuttle Pavilion Opening

NASA Image and Video Library

2012-07-19

Former NASA Astronaut and Enterprise Commander Joe Engle looks at an exhibit in the Intrepid Sea, Air & Space Museum's Space Shuttle Pavilion where the space shuttle Enterprise is on Thursday, July 19, 2012 in New York. Photo Credit: (NASA/Bill Ingalls)

STS-38 Space Shuttle mission report

NASA Technical Reports Server (NTRS)

Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

1991-01-01

The STS-38 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-seventh flight of the Space Shuttle and the seventh flight of the Orbiter vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-40/LWT-33), three Space Shuttle main engines (SSME's) (serial numbers 2019, 2022, 2027), and two Solid Rocket Boosters (SRB's), designated as BI-039. The STS-38 mission was a classified Department of Defense mission, and as much, the classified portions of the mission are not presented in this report. The sequence of events for this mission is shown. The significant problems that occurred in the Space Shuttle Orbiter subsystem during the mission are summarized and the official problem tracking list is presented. In addition, each Space Shuttle Orbiter problem is cited in the subsystem discussion.

Success Legacy of the Space Shuttle Program: Changes in Shuttle Post Challenger and Columbia

NASA Technical Reports Server (NTRS)

Jarrell, George

2010-01-01

This slide presentation reviews the legacy of successes in the space shuttle program particularly with regards to the changes in the culture of NASA's organization after the Challenger and Columbia accidents and some of the changes to the shuttles that were made manifest as a result of the accidents..

The mechanism of RNA 5' capping with NAD +, NADH and desphospho-CoA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bird, Jeremy G.; Zhang, Yu; Tian, Yuan

The chemical nature of the 5' end of RNA is a key determinant of RNA stability, processing, localization and translation efficiency and has been proposed to provide a layer of ‘epitranscriptomic’ gene regulation. Recently it has been shown that some bacterial RNA species carry a 5'-end structure reminiscent of the 5' 7-methylguanylate ‘cap’ in eukaryotic RNA. In particular, RNA species containing a 5'-end nicotinamide adenine dinucleotide (NAD+) or 3'-desphospho-coenzyme A (dpCoA) have been identified in both Gram-negative and Gram-positive bacteria. It has been proposed that NAD+, reduced NAD+ (NADH) and dpCoA caps are added to RNA after transcription initiation, inmore » a manner analogous to the addition of 7-methylguanylate caps. Here we show instead that NAD+, NADH and dpCoA are incorporated into RNA during transcription initiation, by serving as non-canonical initiating nucleotides (NCINs) for de novo transcription initiation by cellular RNA polymerase (RNAP). We further show that both bacterial RNAP and eukaryotic RNAP II incorporate NCIN caps, that promoter DNA sequences at and upstream of the transcription start site determine the efficiency of NCIN capping, that NCIN capping occurs in vivo, and that NCIN capping has functional consequences. We report crystal structures of transcription initiation complexes containing NCIN-capped RNA products. Our results define the mechanism and structural basis of NCIN capping, and suggest that NCIN-mediated ‘ab initio capping’ may occur in all organisms.« less

Evaluation philosophy for shuttle launched payloads

NASA Technical Reports Server (NTRS)

Heuser, R. E.

1975-01-01

Some approaches to space-shuttle payload evaluation are examined. Issues considered include subsystem replacement in low-cost modular spacecraft (LCMS), validation of spacelab payloads, the use of standard components in shuttle-era spacecraft, effects of shuttle-induced environments on payloads, and crew safety. The LCMS is described, and goals are discussed for its evaluation program. Concepts regarding how the evaluation should proceed are considered.

Space Shuttle Usage of z/OS

NASA Technical Reports Server (NTRS)

Green, Jan

2009-01-01

This viewgraph presentation gives a detailed description of the avionics associated with the Space Shuttle's data processing system and its usage of z/OS. The contents include: 1) Mission, Products, and Customers; 2) Facility Overview; 3) Shuttle Data Processing System; 4) Languages and Compilers; 5) Application Tools; 6) Shuttle Flight Software Simulator; 7) Software Development and Build Tools; and 8) Fun Facts and Acronyms.

STS-121 Space Shuttle Processing Update

NASA Image and Video Library

2006-04-27

NASA Administrator Michael Griffin, left, and Associate Administrator for Space Operations William Gerstenmaier, right, look on as Space Shuttle Program Manager Wayne Hale talks from NASA's Marshall Space Flight Center about the space shuttle's ice frost ramps during a media briefing about the space shuttle program and processing for the STS-121 mission, Friday, April 28, 2006, at NASA Headquarters in Washington. Photo Credit (NASA/Bill Ingalls)

STS-80 Space Shuttle Mission Report

NASA Technical Reports Server (NTRS)

Fricke, Robert W., Jr.

1997-01-01

The STS-80 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the eightieth flight of the Space Shuttle Program, the fifty-fifth flight since the return-to-flight, and the twenty-first flight of the Orbiter Columbia (OV-102).

Shuttle mission plans

NASA Technical Reports Server (NTRS)

Visentine, J. T.; Lee, C. M.

1978-01-01

Shuttle mission plans recently developed by NASA for the time period 1980-1991 are presented. Standard and optional services, which will be available to users of the Space Transportation System (STS) when it becomes operational in the 1980's, are described. Pricing policies established by NASA to encourage use of the STS by commercial, foreign and other U.S. Government users are explained. The small Self-Contained Payload Program, which will make space flight opportunities available to private citizens and individual experimenters who wish to use the Space Shuttle for investigative research, is discussed.

Space Shuttle Project

NASA Image and Video Library

1993-04-08

The second try works like a charm as the Space Shuttle Discovery (STS-56) lifts off from Launch Pad 39B. The first attempt to launch was halted at T-11 seconds on April 6th. Aboard for the second shuttle mission of 1993 were a crew of five and the Atmospheric Laboratory for Applications and Science 2 (ATLAS 2), the second in a series of missions to study the sun's energy output and Earth's middle atmosphere chemical make-up, and how these factors affect levels of ozone.

Space Shuttle Project

NASA Image and Video Library

1992-01-22

Onboard Space Shuttle Discovery (STS-42) the seven crewmembers pose for a traditional in-space portrait in the shirt-sleeve environment of the International Microgravity Laboratory (IML-1) science module in the Shuttle's cargo bay. Pictured are (clockwise from top),Commander Ronald J. Grabe, payload commander Norman E. Thagard, payload specialist Roberta L. Bondar; mission specialists William F. Readdy and David C. Hilmers; pilot Stephen S. Oswald and payload specialist Ulf Merbold. The rotating chair, used often in biomedical tests on the eight-day flight, is in center frame.

STS-92 - Towing of Shuttle Discovery and Boeing 747 Shuttle Carrier Aircraft (SCA)

NASA Image and Video Library

2000-11-02

The Space Shuttle Discovery sits atop one of NASA’s modified Boeing 747 Shuttle Carrier Aircraft as the unusual piggyback duo is towed along a taxiway at NASA’s Dryden Flight Research Center at Edwards, California. The Discovery was ferried from NASA Dryden to NASA’s Kennedy Space Center in Florida on November 2, 2000, after extensive pre-ferry servicing and preparations.

Locations of ectopic beats coincide with spatial gradients of NADH in a regional model of low-flow reperfusion.

PubMed

Kay, Matthew; Swift, Luther; Martell, Brian; Arutunyan, Ara; Sarvazyan, Narine

2008-05-01

We studied the origins of ectopic beats during low-flow reperfusion after acute regional ischemia in excised rat hearts. The left anterior descending coronary artery was cannulated. Perfusate was delivered to the cannula using an high-performance liquid chromatography pump. This provided not only precise control of flow rate but also avoided mechanical artifacts associated with vessel occlusion and deocclusion. Optical mapping of epicardial transmembrane potential served to identify activation wavefronts. Imaging of NADH fluorescence was used to quantify local ischemia. Our experiments suggest that low-flow reperfusion of ischemic myocardium leads to a highly heterogeneous ischemic substrate and that the degree of ischemia between adjacent patches of tissue changes in time. In contrast to transient ectopic activity observed during full-flow reperfusion, persistent ectopic arrhythmias were observed during low-flow reperfusion. The origins of ectopic beats were traceable to areas of high spatial gradients of changes in NADH fluorescence caused by low-flow reperfusion.

Space Shuttle Projects Overview to Columbia Air Forces War College

NASA Technical Reports Server (NTRS)

Singer, Jody; McCool, Alex (Technical Monitor)

2000-01-01

This paper presents, in viewgraph form, a general overview of space shuttle projects. Some of the topics include: 1) Space Shuttle Projects; 2) Marshall Space Flight Center Space Shuttle Projects Office; 3) Space Shuttle Propulsion systems; 4) Space Shuttle Program Major Sites; 5) NASA Office of Space flight (OSF) Center Roles in Space Shuttle Program; 6) Space Shuttle Hardware Flow; and 7) Shuttle Flights To Date.

Space Shuttle Projects

NASA Image and Video Library

1997-12-08

The STS-90 crew patch reflects the dedication of the mission to neuroscience in celebration of the decade of the brain. Earth is revealed through a neuron-shaped window, which symbolizes new perspectives in the understanding of nervous system development, structure and function, both here on Earth and in the microgravity environment of space. The Space Shuttle Columbia is depicted with its open payload bay doors revealing the Spacelab within. An integral component of the mission, the laboratory/science module provided by the European Space Agency (ESA), signifies the strong international involvement in the mission. The seven crew members and two alternate payload specialists, Chiaki Naito-Mukai and Alexander W. Dunlap, are represented by the nine major stars of the constellation Cetus (the whale) in recognition of the International Year of the Ocean. The distant stars illustrate the far reaching implications of the mission science to the many sponsoring agencies, helping prepare for long-duration space flight aboard the International Space Station (ISS). The moon and Mars are depicted to reflect the crew's recognition that those two celestial bodies will be the next great challenges in human exploration of space and represent the key role that life science research will play in supporting such missions.

Space Shuttle wind tunnel testing program

NASA Technical Reports Server (NTRS)

Whitnah, A. M.; Hillje, E. R.

1984-01-01

A major phase of the Space Shuttle Vehicle (SSV) Development Program was the acquisition of data through the space shuttle wind tunnel testing program. It became obvious that the large number of configuration/environment combinations would necessitate an extremely large wind tunnel testing program. To make the most efficient use of available test facilities and to assist the prime contractor for orbiter design and space shuttle vehicle integration, a unique management plan was devised for the design and development phase. The space shuttle program is reviewed together with the evolutional development of the shuttle configuration. The wind tunnel testing rationale and the associated test program management plan and its overall results is reviewed. Information is given for the various facilities and models used within this program. A unique posttest documentation procedure and a summary of the types of test per disciplines, per facility, and per model are presented with detailed listing of the posttest documentation.

Shuttle Discovery Landing at Palmdale, California, Maintenance Facility

NASA Technical Reports Server (NTRS)

1995-01-01

NASA Dryden Flight Research Center pilot Tom McMurtry lands NASA's Shuttle Carrier Aircraft with Space Shuttle Discovery attached at Rockwell Aerospace's Palmdale, California, facility about 1:00 p.m. Pacific Daylight Time (PDT). There for nine months of scheduled maintenance, Discovery and the 747 were completing a two-day flight from Kennedy Space Center, Florida, that began at 7:04 a.m. Eastern Standard Time on 27 September and included an overnight stop at Salt Lake City International Airport, Utah. At the conclusion of this mission, Discovery had flown 21 shuttle missions, totaling more than 142 days in orbit. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout the mission. They are then removed after the Space Shuttle returns to Earth and

Space Shuttle ice nuclei

NASA Astrophysics Data System (ADS)

Turco, R. P.; Toon, O. B.; Whitten, R. C.; Cicerone, R. J.

1982-08-01

Estimates are made showing that, as a consequence of rocket activity in the earth's upper atmosphere in the Shuttle era, average ice nuclei concentrations in the upper atmosphere could increase by a factor of two, and that an aluminum dust layer weighing up to 1000 tons might eventually form in the lower atmosphere. The concentrations of Space Shuttle ice nuclei (SSIN) in the upper troposphere and lower stratosphere were estimated by taking into account the composition of the particles, the extent of surface poisoning, and the size of the particles. Calculated stratospheric size distributions at 20 km with Space Shuttle particulate injection, calculated SSIN concentrations at 10 and 20 km altitude corresponding to different water vapor/ice supersaturations, and predicted SSIN concentrations in the lower stratosphere and upper troposphere are shown.

Space Shuttle Discovery Fly-Over

NASA Image and Video Library

2012-04-17

Spectators watch as space shuttle Discovery, mounted atop a NASA 747 Shuttle Carrier Aircraft (SCA) flies over the National Air and Space Museum’s Steven F. Udvar-Hazy Center, Tuesday, April 17, 2012, in Chantilly, Va. Discovery, the first orbiter retired from NASA’s shuttle fleet, completed 39 missions, spent 365 days in space, orbited the Earth 5,830 times, and traveled 148,221,675 miles. NASA will transfer Discovery to the National Air and Space Museum to begin its new mission to commemorate past achievements in space and to educate and inspire future generations of explorers. Photo Credit: (NASA/Carla Cioffi)

A soluble NADH-dependent fumarate reductase in the reductive tricarboxylic acid cycle of Hydrogenobacter thermophilus TK-6.

PubMed