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Sample records for ng-150 opredelenie belka

  1. New scientific equipment for protein crystallization in microgravity, BELKA, and its approbation on the Bion-M No. 1 spacecraft

    SciTech Connect

    Baskakova, S. S. Kovalyov, S. I.; Kramarenko, V. A.; Zadorozhnaya, L. A.; Lyasnikova, M. S.; Dymshits, Y. M.; Shishkov, V. A.; Egorov, A. V.; Dolgin, A. M.; Voloshin, A. E.; Kovalchuk, M. V.

    2015-01-15

    A space experiment on the crystallization of lisozyme and glucose isomerase proteins in UK-1 and UK-2 crystallizers on the scientific equipment BELKA on the Bion-M no. 1 spacecraft was performed in April–May 2013. A ground-based experiment was carried out simultaneously at the Institute of Crystallography of the Russian Academy of Sciences (IC RAS). Transparent crystals were obtained in both cases. The lisozyme crystals grown in microgravity are larger than their terrestrial analogs. An optical study of glucose isomerase crystals grown in space has shown that the coalescence of equally oriented crystallites leads to the formation of quasi-single-crystal blocks. An X-ray diffraction experiment on lisozyme crystals has revealed the resolutions for crystals obtained under terrestrial conditions and in space to be 1.74 and 1.58 Å, respectively.

  2. DETERMINATION OF C1 AND Cx CELLULOLYTIC ACTIVITIES IN ENZYME PREPARATIONS OF MOLD FUNGI (Opredelenie C1 i Cx Tsellyuloliticheskikh Aktivnostei v Fermentnykh Preparatakh iz Plesnevykh Gribov),

    DTIC Science & Technology

    Trichotecium roseum, Aspergillus awamory, Asp. niger, Asp. flavus . Differences in the distribution of C1 - and Cx - activities in the preparations of various strains of the same fungus (Asp. awamory, Asp. oryzae) are shown. (Author)

  3. Large Extremity Peripheral Nerve Repair

    DTIC Science & Technology

    2016-12-01

    recovery after delayed nerve repair: Prolonged axotomy. J Neurosci. 1995;15:3876–3885. 4. Furey MJ, Midha R , Xu QG, Belkas J , Gordon T . Prolonged tar...2106. Goldstein R 15. , Runyan G, Randolph MA, Easow J , Meppelink A, Fahradyan V, Winograd JM, Robert Redmond RW. Photochemical tissue bonding...Surgery Research Council. Durham, NC, May 4-7, 2017. Goldstein R , Runyan G, Randolph MA, Easow J , Meppelink A, Fahradyan V, Winograd JM, Robert

  4. A zircon vs titanite geochronometres by SHRIMP IIe as a tool in multistage magmatic intrusion problems

    NASA Astrophysics Data System (ADS)

    Wiszniewska, Janina; Krzemińska, Ewa

    2017-04-01

    reveal a relatively low uranium contents of about 11-30 ppm resulting in less precise U-Pb apparent ages overlapping 40 Ma (intercept age 1449 ±40 Ma). This age seems to be too young to be interpreted as a simple cooling age. The closure temperature (Tc) for the U-Pb system in titanite is assumed on the level of about 700°C. This younger and imprecise titanite age corresponds however with the youngest expressions of magmatism in this region, documented mostly within Lithuanian crystalline basement, where AMCG plutonic components are known from the Nemunas - Geluva granite suite with U-Pb (SIMS) zircon age in range of 1447 -1445±8Ma ( Skridalite et al. 2007). Dörr W., Belka Z., Marheine D., Schastok J., Valverde-Vaquero P., Wiszniewska J., 2002. U-Pb and Ar-Ar geochronology of anorogenic granite magmatism of the Mazury Complex, NE Poland. Precambrian Research, 119, 101-120. Skridlaite G. Whitehouse M. Rimsa A. 2007 Evidence for a pulse of 1.45 Ga anorthosite-mangerite-charnockite-granite (AMCG) plutonism in Lithuania: implications for the Mesoproterozoic evolution of the East European Craton, Terra Nova 19, 294-301. This is a contribution to the project; "Isotopic parameters of the Suwalki Anorthosite massif - an attempt to explain multistage Fe-Ti-V ore intrusion generation using titanite and apatite geochronometres by SHRIMP IIe " 61.2116.1601.09.0

  5. Petrographic and geochemical analysis of the Givetian-Frasnian sandstones in the Kuh-e-Tizi section, southeastern Central Iran

    NASA Astrophysics Data System (ADS)

    Hejazi, S. H.; Adabi, M. H.; Moussavi Harami, S. R.; Khosro Tehrani, K.

    2009-04-01

    .P., Ramasamy, S., 2004. Geochemistry of sandstones from the Upper Miocene Kudankulam Formation, southern India: implication for provenance, weathering and tectonic setting. J. Sediment. Res. 74, 285-297. Basu, A., Young, S.W., Suttner, L.J., James, W.C., Mack, G.H., 1975. Re-evaluation of the use of undulatory extinction and polycrystallinity in detrital quartz for provenance interpretation. J. Sed. Petrol. 45, 873-882. Bhatia, M.R., 1983. Plate tectonics and geochemical composition of sandstones. J. Geol. 91, 611-627. Bhatia, M.R., Crook, K.A.W., 1986. Trace element characteristics of graywackes and tectonic setting discrimination of sedimentary basins. Contrib. Mineral. Petrol. 92, 181-193. Dickinson, W.R., Beard, L.S., Brakenridge, G.R., Erjavec, J.L., Ferguson, R.C., Inman, K.F., Knepp, R.A., Lindberg, F.A., Ryberg, P.T., 1983. Provenance of North American Phanerozoic sandstones in relation to tectonic setting. Bull. Am. Geol. Soc. 94, 222-235. Kroonenberg, S.B., 1994. Effects of provenance, sorting and weathering on the geochemistry of fluvial sands from different tectonic and climatic environments. Proceedings of the 29th International Geological Congress, Part A, 69-81. Roser, B.P., Korsch, R.J., 1986. Determination of tectonic setting of sandstonemudstone suites using SiO2 content and K2O/Na2O ratio. J. Geol. 94, 635-650. Wendt, J., Kaufmann, B., Belka, Z., Farsan, N. & Karimi bavandpur, A. 2002. Devonian/Lower Carboniferous stratigraphy, facies patterns and palaeogeography of Iran. Part I. Southeastern Iran. Acta Geol. Polo. 52, 129-168.

  6. Development and Validation of a Sensitive Enzymeimmunoassay for Determination of Plasma Metastin in Mithun (Bos frontalis).

    PubMed

    Mondal, Mohan; Karunakaran, M; Baruah, Kishore Kumar

    2016-01-01

    Metastin, also known as kisspeptin-10, is a potent stimulator of gonadotropin-releasing hormone (GnRH) neurons in the central nervous system. Recently, it has been emerged as a key player in the regulation of reproduction in mammals. Blood concentrations of metastin during different physiological stages in bovine species in general and mithun (Bos frontalis) in particular are not available. Lacking of such information may probably be due to non-availability of simple assay procedure to measure the peptide. Therefore, the objective of this study was to develop and validate a simple and sufficiently sensitive enzyme immunoassay (EIA) for metastin determination in mithun plasma using the biotin-streptavidin amplification system and second antibody coating technique. Biotin was coupled to metastin and used to bridge between streptavidin-peroxidase and the immobilized metastin antiserum in the competitive assay. The EIA was conducted directly in 150 μ L of unknown mithun plasma. Metastin standards ranging from 0.01-51.2 ng/150 μ L/well were prepared in hormone-free plasma. The lowest detection limit was 0.07 ng/mL plasma. Plasma volumes for the EIA, viz., 75, 150, and 200 μ L did not influence the shape of standard curve even though a drop in OD450 was seen with higher plasma volumes. A parallelism test was carried out to compare the endogenous mithun metastin with metastin standard used. It showed good parallelism with the metastin standard curve. For the biological validation of the assay, metastin was measured in (a) blood samples collected from 12 pregnant mithun cows during different stages of pregnancy, (b) in blood from seven early pregnant and 12 non-pregnant mithuns, and (c) in follicular fluid obtained from different types of follicle. It was found that the plasma metastin concentrations increased (P < 0.001) from first through last trimester of pregnancy. Plasma metastin levels were much higher (P < 0.001) in early pregnant than non-pregnant cows