Science.gov

Sample records for nick kettridge andrew

  1. Be Like Nick.

    ERIC Educational Resources Information Center

    Smith, Paul Chaat

    1996-01-01

    Discusses controversy surrounding books supposedly written by and about American Indians that are fabrications written by whites. Stresses the importance of moving away from American Indian literature that trivializes Indian culture and values, toward more realistic portrayals. Black Elk, whose first name was Nick, is an example of how cultural…

  2. Interview with Deborah Andrews

    ERIC Educational Resources Information Center

    Ryan, Cynthia

    2005-01-01

    This article presents an interview with Deborah Andrews about her experiences during her editorship of "Business Communication Quarterly." From June 1997 to March 2005, Debby served as editor of the journal, encouraging all readers to ask important questions about their work: How should we define business communication? On which disciplines and…

  3. Andrew W. S. In

    ERIC Educational Resources Information Center

    Nishimoto, Warren

    2007-01-01

    This article presents an interview with Andrew W. S. In, professor and dean in the University of Hawai'i's College of Education from 1951 to 1984. Born and raised in Honolulu, In attended Royal Elementary, Central Junior High, and McKinley High schools, graduating from McKinley in 1938. He then attended the University of Hawai'i Teachers College…

  4. Andrew: CMU's New Computing Environment.

    ERIC Educational Resources Information Center

    Zabowski, Susan

    1986-01-01

    Reviews the progress and problems associated with the development of Carnegie Mellon University's new computing and communications system, "Andrew." Describes the accomplishments and capacities of the system and provides examples of the programs developed for "Andrew." (ML)

  5. Andrew integrated reservoir description

    SciTech Connect

    Todd, S.P.

    1996-12-31

    The Andrew field is an oil and gas accumulation in Palaeocene deep marine sands in the Central North Sea. It is currently being developed with mainly horizontal oil producers. Because of the field`s relatively small reserves (mean 118 mmbbls), the performance of each of the 10 or so horizontal wells is highly important. Reservoir description work at sanction time concentrated on supporting the case that the field could be developed commercially with the minimum number of wells. The present Integrated Reservoir Description (IRD) is focussed on delivering the next level of detail that will impact the understanding of the local reservoir architecture and dynamic performance of each well. Highlights of Andrew IRD Include: (1) Use of a Reservoir Uncertainty Statement (RUS) developed at sanction time to focus the descriptive effort of both asset, support and contract petrotechnical staff, (2) High resolution biostratigraphic correlation to support confident zonation of the reservoir, (3) Detailed sedimentological analysis of the core including the use of dipmeter to interpret channel/sheet architecture to provide new insights into reservoir heterogeneity; (4) Integrated petrographical and petrophysical investigation of the controls on Sw-Height and relative permeability of water; (5) Fluids description using oil geochemistry and Residual Salt Analysis Sr isotope studies. Andrew IRD has highlighted several important risks to well performance, including the influence of more heterolithic intervals on gas breakthrough and the controls on water coning exerted by suppressed water relative permeability in the transition zone.

  6. Andrew integrated reservoir description

    SciTech Connect

    Todd, S.P.

    1996-01-01

    The Andrew field is an oil and gas accumulation in Palaeocene deep marine sands in the Central North Sea. It is currently being developed with mainly horizontal oil producers. Because of the field's relatively small reserves (mean 118 mmbbls), the performance of each of the 10 or so horizontal wells is highly important. Reservoir description work at sanction time concentrated on supporting the case that the field could be developed commercially with the minimum number of wells. The present Integrated Reservoir Description (IRD) is focussed on delivering the next level of detail that will impact the understanding of the local reservoir architecture and dynamic performance of each well. Highlights of Andrew IRD Include: (1) Use of a Reservoir Uncertainty Statement (RUS) developed at sanction time to focus the descriptive effort of both asset, support and contract petrotechnical staff, (2) High resolution biostratigraphic correlation to support confident zonation of the reservoir, (3) Detailed sedimentological analysis of the core including the use of dipmeter to interpret channel/sheet architecture to provide new insights into reservoir heterogeneity; (4) Integrated petrographical and petrophysical investigation of the controls on Sw-Height and relative permeability of water; (5) Fluids description using oil geochemistry and Residual Salt Analysis Sr isotope studies. Andrew IRD has highlighted several important risks to well performance, including the influence of more heterolithic intervals on gas breakthrough and the controls on water coning exerted by suppressed water relative permeability in the transition zone.

  7. Faces of Marshall: Erika Andrews

    NASA Video Gallery

    Several Marshall employees were interviewed as part of Marshall's 50th Anniversary activities. Human Resources Specialist Erika Andrews tells how she came to work at NASA as a specialist in organiz...

  8. Benevolent Builder: Appraising Andrew Carnegie.

    ERIC Educational Resources Information Center

    Deitch, Joseph

    1984-01-01

    Discussion of Andrew Carnegie era in American public library history focuses on library buildings financed and built in Philadelphia, Pennsylvania. The building design which Carnegie developed, surviving Carnegie libraries in Philadelphia, procedures for obtaining a library, and comparison of architectural attractiveness of Carnegie libraries with…

  9. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks.

    PubMed

    Vriend, Lianne E M; Prakash, Rohit; Chen, Chun-Chin; Vanoli, Fabio; Cavallo, Francesca; Zhang, Yu; Jasin, Maria; Krawczyk, Przemek M

    2016-06-20

    DNA double-strand breaks (DSBs) are known to be powerful inducers of homologous recombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR. Both DSB- and nick-induced HR ((nick)HR) are exploited in advanced genome-engineering approaches based on the bacterial RNA-guided nuclease Cas9. However, the mechanisms of (nick)HR are largely unexplored. Here, we applied Cas9 nickases to study (nick)HR in mammalian cells. We find that (nick)HR is unaffected by inhibition of major damage signaling kinases and that it is not suppressed by nonhomologous end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to trigger HR. Relative to a single nick, nicking both strands enhances HR, consistent with a DSB intermediate, even when nicks are induced up to ∼1kb apart. Accordingly, HR and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated. Our study advances the understanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena associated with Cas9-mediated genome editing.

  10. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks

    PubMed Central

    Vriend, Lianne E.M.; Prakash, Rohit; Chen, Chun-Chin; Vanoli, Fabio; Cavallo, Francesca; Zhang, Yu; Jasin, Maria; Krawczyk, Przemek M.

    2016-01-01

    DNA double-strand breaks (DSBs) are known to be powerful inducers of homologous recombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR. Both DSB- and nick-induced HR (nickHR) are exploited in advanced genome-engineering approaches based on the bacterial RNA-guided nuclease Cas9. However, the mechanisms of nickHR are largely unexplored. Here, we applied Cas9 nickases to study nickHR in mammalian cells. We find that nickHR is unaffected by inhibition of major damage signaling kinases and that it is not suppressed by nonhomologous end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to trigger HR. Relative to a single nick, nicking both strands enhances HR, consistent with a DSB intermediate, even when nicks are induced up to ∼1kb apart. Accordingly, HR and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated. Our study advances the understanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena associated with Cas9-mediated genome editing. PMID:27001513

  11. Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA

    PubMed Central

    Gutjahr, Alice; Xu, Shuang-yong

    2014-01-01

    N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated fusion enzymes that preferentially nick 5mCG-modified DNA. The CG dinucleotide can be modified by C5 methyltransferases (MTases) such as M.SssI, M.HhaI or M.HpaII to create composite sites AC↑YGG N(8–15) 5mCG. We also constructed a fusion enzyme 2xMBD2-ND(N.BceSVIII) targeting more frequent composite sites AS↑YS N(5–12) 5mCG in Mn2+ buffer. 5mCG-dependent nicking requires special digestion conditions in high salt (0.3 M KCl) or in Ni2+ buffer. The fusion enzyme can be used to nick and label 5mCG-modified plasmid and genomic DNAs with fluorescently labeled Cy3-dUTP and potentially be useful for diagnostic applications, DNA sequencing and optical mapping of epigenetic markers. The importance of the predicted catalytic residues D89, H90, N106 and H115 in N.ϕGamma was confirmed by mutagenesis. We found that the wild-type enzyme N.ϕGamma prefers to nick 5mCG-modified DNA in Ni2+ buffer even though the nicking activity is sub-optimal compared to the activity in Mg2+ buffer. PMID:24609382

  12. Site-specific nicking within the adenovirus inverted terminal repetition.

    PubMed Central

    Chow, K C; Pearson, G D

    1984-01-01

    Site-specific nicking occurs on the l-strand, but not on the r-strand, of the adenovirus left inverted terminal repeat. Nicks are presumably introduced into double- or single-stranded DNA by a cellular endonuclease in an ATP-independent reaction. The consensus nick site has the sequence: (sequence in text). Images PMID:6322107

  13. The McAndrews Leadership Lecture: Origins

    PubMed Central

    Hamm, Anthony W.; Burkhart, Lori A.

    2015-01-01

    Objective This article describes the origins and rationale for the McAndrews Leadership Lecture and explains why the American Chiropractic Association honors George and Jerome McAndrews. Discussion George and Jerome McAndrews’ backgrounds demonstrate their leadership contributions to the chiropractic profession. Jerome McAndrews, a chiropractor, held substantial leadership roles in the chiropractic profession. George McAndrews, a lawyer, administered a permanent injunction forbidding the American Medical Association’s restraint of trade toward the chiropractic profession. Conclusion The American Chiropractic Association has established the McAndrews Leadership Lecture to honor their contributions to the chiropractic profession. PMID:26770176

  14. The Impeachment of Andrew Johnson. Web Lesson.

    ERIC Educational Resources Information Center

    Constitutional Rights Foundation, Los Angeles, CA.

    This lesson presents the historical background of Abraham Lincoln's selection of Andrew Johnson as his running mate in the election of 1864. The lesson considers the climate in the U.S. Congress after President Lincoln's assassination. The details of the impeachment and trial of President Andrew Johnson are given. The lesson presents three…

  15. Obituary: Andrew Stephen Wilson, 1947-2008

    NASA Astrophysics Data System (ADS)

    Veilleux, Sylvain

    2009-01-01

    On 24 May 2008, Andrew Stephen Wilson passed away at the age of 61, in his home in Silver Spring, Maryland, from complications resulting from a painful spinal illness. Andrew was arguably one of the first truly multi-wavelength astronomers of his generation. His scientific work on active galactic nuclei [AGN] spanned the entire electromagnetic spectrum from the radio to the X-rays. Andrew was born in Doncaster, Yorkshire, England, on 26 March 1947. He was the younger of two brothers whose births were separated by the Second World War. His father, Norman, came from a relatively affluent family who were coal merchants. His mother, Mary, came from a less comfortable background, one of seven children, daughter of a skilled cabinet maker/French polisher, who went through a very hard time during the depression. As a teacher, she placed enormous value on hard work and education as a way of gaining advancement in life. When Andrew was four, the family moved to Skipton, a nice market town in the Yorkshire dales. Andrew went to a small village school until age eleven when he entered Ermysted's Grammar School. He was an enthusiastic soccer and cricket player. He never lost his enthusiasm for soccer and supported the local soccer team, Leeds United, for all his life. Andrew also followed the Yorkshire county cricket team. Andrew's interest in astronomy stemmed from the fact that at Ermysted's Grammar School someone donated a four-inch refracting telescope, so he and his friends used to go back in the evenings to investigate the rings of Saturn, the moons of Jupiter, and various nebulae. While an undergraduate at Cambridge, Andrew joined the astronomy club and ground an 8-inch mirror by hand as a part of a telescope that he set up in the backyard of his parents' house. Andrew spent hours observing with this telescope, and it was the wonder of the family. At Cambridge, Andrew obtained his bachelor's degree with first-class honors in 1969. During a short visit in London with his

  16. Cell scientist to watch - Andrew Holland.

    PubMed

    2017-02-01

    Andrew received his first degree in natural sciences from the University of Cambridge and a Masters degree from the University of Manchester, followed by a PhD with Stephen Taylor in Manchester. He then moved to California in 2007 with an EMBO long-term fellowship for his postdoctoral research with Don Cleveland at the Ludwig Institute for Cancer Research. In 2013, Andrew started his own lab as an Assistant Professor in the Department of Molecular Biology and Genetics at the Johns Hopkins University School of Medicine, having been named a Kimmel Scholar and a Pew-Stewart Scholar in 2014. Andrew's lab investigates the mechanisms controlling centrosome copy numbers during cell division and the links between centrosome amplification, genome instability and tumorigenesis.

  17. Congruences for the Andrews spt function

    PubMed Central

    Ono, Ken

    2011-01-01

    Ramanujan-type congruences for the Andrews spt(n) partition function have been found for prime moduli 5 ≤ ℓ ≤ 37 in the work of Andrews [Andrews GE, (2008) J Reine Angew Math 624:133–142] and Garvan [Garvan F, (2010) Int J Number Theory 6:1–29]. We exhibit unexpectedly simple congruences for all ℓ≥5. Confirming a conjecture of Garvan, we show that if ℓ≥5 is prime and , then (mod ℓ). This congruence gives (ℓ - 1)/2 arithmetic progressions modulo ℓ3 which support a mod ℓ congruence. This result follows from the surprising fact that the reduction of a certain mock theta function modulo ℓ, for every ℓ≥5, is an eigenform of the Hecke operator T(ℓ2). PMID:21177432

  18. Automated quantification of retinal arteriovenous nicking from colour fundus images.

    PubMed

    Nguyen, Uyen T V; Bhuiyan, Alauddin; Park, Laurence A F; Kawasaki, Ryo; Wong, Tien Y; Wang, Jie J; Mitchell, Paul; Ramamohanarao, Kotagiri

    2013-01-01

    Retinal arteriovenous nicking (AV nicking) is the phenomenon where the venule is compressed or decreases in its caliber at both sides of an arteriovenous crossing. Recent research suggests that retinal AVN is associated with hypertension and cardiovascular diseases such as stroke. In this article, we propose a computer method for assessing the severity level of AV nicking of an artery-vein (AV) crossing in color retinal images. The vascular network is first extracted using a method based on multi-scale line detection. A trimming process is then performed to isolate the main vessels from unnecessary structures such as small branches or imaging artefact. Individual segments of each vessel are then identified and the vein is recognized through an artery-vein identification process. A vessel width measurement method is devised to measure the venular caliber along its two segments. The vessel width measurements of each venular segment is then analyzed and assessed separately and the final AVN index of a crossover is computed as the most severity of its two segments. The proposed technique was validated on 69 AV crossover points of varying AV nicking levels extracted from retinal images of the Singapore Malay Eye Study (SiMES). The results show that the computed AVN values are highly correlated with the manual grading with a Spearman correlation coefficient of 0.70. This has demonstrated the accuracy of the proposed method and the feasibility to develop a computer method for automatic AV nicking detection. The quantitative measurements provided by the system may help to establish a more reliable link between AV nicking and known systemic and eye diseases, which deserves further examination and exploration.

  19. Oxygen and Metastasis: A Conversation with Dr. Nick Restifo

    Cancer.gov

    Dr. Nick Restifo, a senior investigator in NCI’s Center for Cancer Research, discusses his recently published study finding that Oxygen, a molecule necessary for life, paradoxically aids cancer metastasis to the lung by impairing cancer-killing immune cells.

  20. Obituary: Andrew Lange (1957-2010)

    NASA Astrophysics Data System (ADS)

    Kamionkowski, Marc

    2011-12-01

    The worlds of physics and astrophysics were stunned to learn on 22 January 2010 that Andrew Lange, the Marvin L. Goldberger Professor of Physics at Caltech, had taken his own life the night before. He had succumbed to the severe depression that he had suffered from for many years, unbeknownst to even his closest colleagues. Lange will perhaps be best remembered as the co-leader of Boomerang, the balloon-borne experiment that provided the first high-angular-resolution map of the cosmic microwave background (CMB). And while this was certainly his most notable achievement, Andrew amassed a record of accomplishment as an instrumentalist, leader, mentor, and communicator that extended much further. Andrew was born in Urbana, Illinois on July 23, 1957, the son of an architect and a librarian, and raised primarily in Connecticut. His family and early friends remember him as a serious and extremely intelligent child and young man. Andrew Lange's lifelong interest in the CMB was nurtured as an undergraduate at Princeton University by David Wilkinson, and he recalled fondly a summer spent working with John Mather at Goddard Space Flight Center. Andrew Lange went to graduate school in physics at Berkeley where he worked in Paul Richards' group. Although his thesis project, the Berkeley-Nagoya rocket experiment, showed an anomalous sub-millimeter excess in the CMB spectrum that was shortly thereafter shown by a later flight of the same rocket and COBE-FIRAS to be incorrect, Lange's talents were recognized by the physics department at Berkeley who appointed him shortly after his PhD (1987) to their faculty. While on the Berkeley faculty, Andrew obtained early detections of the Sunyaev-Zeldovich effect, upper limits to small-angle CMB fluctuations, and important infrared constraints to the interstellar medium. He also led a pioneering instrument operating 300 mK detectors for a small infrared satellite experiment. This early work showed high ambition and daring, and it pioneered

  1. The human side of Hurricane Andrew

    SciTech Connect

    Marshall, R.; Callander, R.C.

    1994-12-31

    This paper examines the long-term psychological effects of the nation`s worst natural disaster on the employees of the Turkey Point nuclear power plant. It also examines the efforts made by plant personnel and company volunteers to aid employees` families affected by the storm. Despite significant damage at the plant, unit 4 was returned to service 5 weeks after the August 24, 1992, hurricane. Unit 3 was returned to service on December 3, 1992. Unit 3 was originally scheduled to start a refueling outage the day Hurricane Andrew struck. While plant personnel are still recovering from Andrew`s impact, the plant`s performance has never been better. On May 26, 1993, the plant completed a record-breaking 46-day refueling outage - 7 days ahead of schedule and $3 million under budget. Turkey Point`s recovery, return to service, and superior performance would not have been possible without the efforts of hundreds of employees who put their personal tragedies aside and focused on the common goal of the plant`s operation. To help employees with rebuilding their lives, the plant launched extensive assistance programs. Although the plant returned to normal operation, plant personnel continue to struggle in a community whose infrastructure (homes, schools, stores, etc.) have been almost eliminated.

  2. Andrew shortens lifetime of Louisiana Barrier Islands

    NASA Astrophysics Data System (ADS)

    Bush, Susan

    Because the Isles Dernieres, a series of four barrier islands off the coast of Louisiana, have one of the most rapidly eroding shorelines in the world, geologists at the U.S. Geological Survey and the Louisiana Geological Survey have been monitoring erosion activity over the last several years, said Jeff Williams of the USGS in Reston, Va. Hurricane Andrew, which struck the state on August 26, caused severe erosional damage to these islands that has shortened their lifetimes.Before Andrew struck, geologists projected that Raccoon Island would disappear below sea level by the year 2001 and that Whiskey Island would disappear by 2016. Now, due to the severe erosion from Hurricane Andrew, the scientists claim that the islands may disappear before the turn of the century, and the other islands in the Dernieres chain are expected to follow suit within 2 decades. Raccoon, Whiskey, Trinity, and East islands make up the Isles Dernieres, which existed as one island, known as the Isle Derniere, before an 1856 hurricane and subsequent erosion.

  3. 76 FR 48168 - Andrew K. Choi: Debarment Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-08

    ... HUMAN SERVICES Food and Drug Administration Andrew K. Choi: Debarment Order AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is issuing an order under the Federal Food, Drug, and Cosmetic Act (the FD&C Act) debarring Andrew K. Choi, M.D. for 4...

  4. Introduction of Dr. Andrew V Schally

    PubMed Central

    Mendoza-Valdés, Arturo

    2015-01-01

    I first met Dr. Andrew V Schally (PhD, MDhc (Multi), DSc, Distinguished Medical Research Scientist, U.S. Department of Veterans Affairs Professor of Pathology and Department of Medicine,
Miller School of Medicine, University of Miami, Miami, Florida, USA) many years ago, probably around the beginning of the 1990's in one of his visits to Mexico City (Figure 1). He has many friends in my country since some of the investigations that led to the development of the LHRH agonists were made in a couple of Mexican hospitals in collaboration with some outstanding Mexican physicians that I will mention later. In that time, I was the head of the Department of Urology of the Mexican National Cancer Institute and our Director, Dr. Jaime de la Garza, invited him for a meeting. I was surprised by his humbleness, intelligence and easy going personality, in spite of being a Nobel Prize scientist. PMID:26112485

  5. Structural Basis for Nick Recognition by a Minimal Pluripotent DNA Ligase

    SciTech Connect

    Nair,P.; Nandakumar, J.; Smith, P.; Odell, M.; Lima, C.; Shuman, S.

    2007-01-01

    Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-{angstrom} crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO{sub 4} nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a {beta}-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO{sub 4} termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

  6. The complete bending energy function for nicked DNA

    NASA Astrophysics Data System (ADS)

    Qu, Hao; Zocchi, Giovanni

    2011-04-01

    We derive an analytic expression for the bending elastic energy of short DNA molecules, valid in the entire range from low to high energies. The elastic energy depends on three parameters: the length of the molecule (2L), the bending modulus B, and a critical torque τc at which the molecule develops a kink. In the kinked state, the elastic energy is linear in the kink angle, i.e. the torque at the kink is constant (=τc). τc depends (weakly) on the sequence around the nick, but is about 27 pN×nm. We measure it for a specific sequence, through experiments where the elastic energy of constrained DNA molecules is directly measured.

  7. Fred Plaut in conversation with Andrew Samuels. Interview by Andrew Samuels.

    PubMed

    Plaut, Fred

    2010-02-01

    This is a reprint of an interview of Fred Plaut (who died in June 2009) conducted by Andrew Samuels in mid-1988 and first published in April 1989 in the Journal, 34, 2, pp. 159-83. The interview covers Plaut's early life, his career, and historical observations of the development of the Society of Analytical Psychology from its beginnings, and of the wider community of Jungian analysis. Plaut reflects uninhibitedly on such topics as the role of leadership in analytical psychology, discussing the parts played by Michael Fordham in London and Hannes Dieckmann in Berlin. Plaut explains his thinking concerning individuation.

  8. 3. Historic American Buildings Survey L. D. Andrew, Photographer Nov. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Nov. 27, 1936 FRONT DOORWAY; INTERIOR VIEW - Kilpatrick House, Forrest Hills Road (moved from Greene Street), Augusta, Richmond County, GA

  9. 5. Historic American Buildings Survey L. D. Andrew, Photographer Nov. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer Nov. 27, 1936 MANTEL IN RIGHT FRONT ROOM - Kilpatrick House, Forrest Hills Road (moved from Greene Street), Augusta, Richmond County, GA

  10. 5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 DETAIL OF DOUBLE OPENING WITH IRON GRATING IN THIRD STORY REAR ROOM - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  11. 3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 PANELS AND VAULTED CEILING IN DRAWING ROOM, SECOND FLOOR - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  12. 2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From photo of Miss Edith Johnston, Savannah, Ga. 1936 VIEW OF FRONT AND RIGHT SIDE (Restoration 1936) - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  13. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From Photo of Miss Edith Johnston's, Savannah, Ga. 1936 VIEW OF FRONT AND SIDE (Before Restoration, 1936). - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  14. 5. Historic American Buildings Survey L. D. Andrew, Photographer From ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer From photograph furnished by Miss Edith D. Johnston, Savannah, Ga. Sept. 5, 1936 RED BRICK CHURCH (FORMER BUILDING 1811- 1837) - Christ Church (Episcopal), 28 Bull Street, Savannah, Chatham County, GA

  15. 1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged by) Aug. 6, 1936 Photographed by Harold Bush-Brown SIDE VIEW - Covered Bridge, Spanning Soap Creek, Atlanta, Fulton County, GA

  16. 2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM MORRISON'S CAF? (CORNER OF WHITAKER & CONGRESS) SHOWING S. FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  17. 3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM CORNER OF WHITAKER AND ST JULIAN SHOWING NORTH FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  18. 9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW PHOTOGRAPHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW - PHOTOGRAPHER SEPT. 7, 1936 BEAM, CORNICE, AND CEILING ORNAMENT IN FRONT HALL - Sorrel-Weed House, 6 West Harris Street, Savannah, Chatham County, GA

  19. 7. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 CORNER OF WEST FRONT ROOM (SHOWING CORNICE AND WINDOW HEAD) - Wayne-Gordon House, 10 East Oglethorpe Avenue, Savannah, Chatham County, GA

  20. 3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 CENTRAL PORTION OF REAR (Showing backsteps, stoop & stairhall, window) - Wayne-Gordon House, 10 East Oglethorpe Avenue, Savannah, Chatham County, GA

  1. 13. Circa 1880 view (from the north) across Andrew Hallidie ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Circa 1880 view (from the north) across Andrew Hallidie suspension bridge. Source: Searles Library, Nevada City, California. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA

  2. Aortic valve replacement with the Nicks annulus enlargement procedure 12 years after mitral valve replacement.

    PubMed

    Kazama, S; Kurata, A; Yamashita, Y

    1999-10-01

    An aortic valve replacement was successfully performed employing the Nicks annulus enlargement procedure in a case of aortic valve stenosis with small annulus 12 years after mitral valve replacement. Previous mitral valve replacement does not preclude feasibility of the Nicks procedure.

  3. DNA Fragments Assembly Based on Nicking Enzyme System

    PubMed Central

    Wang, Rui-Yan; Shi, Zhen-Yu; Guo, Ying-Ying; Chen, Jin-Chun; Chen, Guo-Qiang

    2013-01-01

    A couple of DNA ligation-independent cloning (LIC) methods have been reported to meet various requirements in metabolic engineering and synthetic biology. The principle of LIC is the assembly of multiple overlapping DNA fragments by single-stranded (ss) DNA overlaps annealing. Here we present a method to generate single-stranded DNA overlaps based on Nicking Endonucleases (NEases) for LIC, the method was termed NE-LIC. Factors related to cloning efficiency were optimized in this study. This NE-LIC allows generating 3′-end or 5′-end ss DNA overlaps of various lengths for fragments assembly. We demonstrated that the 10 bp/15 bp overlaps had the highest DNA fragments assembling efficiency, while 5 bp/10 bp overlaps showed the highest efficiency when T4 DNA ligase was added. Its advantage over Sequence and Ligation Independent Cloning (SLIC) and Uracil-Specific Excision Reagent (USER) was obvious. The mechanism can be applied to many other LIC strategies. Finally, the NEases based LIC (NE-LIC) was successfully applied to assemble a pathway of six gene fragments responsible for synthesizing microbial poly-3-hydroxybutyrate (PHB). PMID:23483947

  4. Interview: Professor Andrew Feinberg speaks to Epigenomics.

    PubMed

    Feinberg, Andrew

    2009-10-01

    Andrew Feinberg studied mathematics and humanities at Yale University (CT, USA) in the Directed Studies honors program, and he received his BA (1973) and MD (1976) from the accelerated medical program at Johns Hopkins University (MD, USA), as well as an MPH from Johns Hopkins (1981). He performed a postdoctoral fellowship in developmental biology at the University of California, San Diego (UCSD, CA, USA), clinical training in medicine and medical genetics at the University of Pennsylvania (PA, USA) and genetics research with Bert Vogelstein at Johns Hopkins, discovering altered DNA methylation in human cancer. Dr Feinberg continued to perform seminal work in cancer epigenetics as a Howard Hughes investigator at the University of Michigan (MI, USA), discovering human imprinted genes and loss of imprinting in cancer, and the molecular basis of Beckwith-Wiedemann syndrome. He returned to John Hopkins in 1994 as King Fahd Professor of Medicine, Molecular Biology & Genetics and Oncology, and he holds an Adjunct Professorship at the Karolinska Institute in Sweden. Dr Feinberg is Director of the Center for Epigenetics, a National Human Genome Research Institute-designated Center of Excellence in Genome Sciences. The Center is pioneering genome-scale tools in molecular, statistical and epidemiological epigenetics, and is applying them to the study of cancer, neuropsychiatric disease and aging. As part of the center, Dr Feinberg has organized a highly innovative program to bring gifted minority high-school students into genetics and genomics. Dr Feinberg has also invented a number of widely used molecular tools, including random priming. His honors include election to the American Society for Clinical Investigation, the Association of American Physicians, the Institute of Medicine of the National Academy of Sciences, and the American Academy of Arts and Sciences, as well as membership on the ISI most-cited authors list, a MERIT Award of the National Cancer Institute, a

  5. NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells

    SciTech Connect

    Shoji, Wataru; Suenaga, Yusuke; Kaneko, Yoshiki; Islam, S.M. Rafiqul; Alagu, Jennifer; Yokoi, Sana; Nio, Masaki; Nakagawara, Akira

    2015-06-05

    NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas. - Highlights: • NCYM promotes cleavages of MYC and MYCN to produce Myc-nick in vitro. • NCYM increases Myc-nick production in MYCN-amplified neuroblastoma cells. • NCYM knockdown decreases Myc-nick production and induces apoptosis at G2/M phase.

  6. Force and twist dependence of RepC nicking activity on torsionally-constrained DNA molecules

    PubMed Central

    Pastrana, Cesar L.; Carrasco, Carolina; Akhtar, Parvez; Leuba, Sanford H.; Khan, Saleem A.; Moreno-Herrero, Fernando

    2016-01-01

    Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires sequence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding of the duplex prior to subsequent leading strand DNA synthesis. Nicking is performed by a replication-initiation protein (Rep) that directly binds to the plasmid double-stranded origin and remains covalently bound to its substrate 5′-end via a phosphotyrosine linkage. It has been proposed that the inverted DNA sequences at the nick site form a cruciform structure that facilitates DNA cleavage. However, the role of Rep proteins in the formation of this cruciform and the implication for its nicking and religation functions is unclear. Here, we have used magnetic tweezers to directly measure the DNA nicking and religation activities of RepC, the replication initiator protein of plasmid pT181, in plasmid sized and torsionally-constrained linear DNA molecules. Nicking by RepC occurred only in negatively supercoiled DNA and was force- and twist-dependent. Comparison with a type IB topoisomerase in similar experiments highlighted a relatively inefficient religation activity of RepC. Based on the structural modeling of RepC and on our experimental evidence, we propose a model where RepC nicking activity is passive and dependent upon the supercoiling degree of the DNA substrate. PMID:27488190

  7. MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

    PubMed Central

    Anderson, Sarah; Poudel, Kumud Raj; Roh-Johnson, Minna; Brabletz, Thomas; Yu, Ming; Borenstein-Auerbach, Nofit; Grady, William N.; Bai, Jihong; Moens, Cecilia B.; Eisenman, Robert N.; Conacci-Sorrell, Maralice

    2016-01-01

    MYC-nick is a cytoplasmic, transcriptionally inactive member of the MYC oncoprotein family, generated by a proteolytic cleavage of full-length MYC. MYC-nick promotes migration and survival of cells in response to chemotherapeutic agents or withdrawal of glucose. Here we report that MYC-nick is abundant in colonic and intestinal tumors derived from mouse models with mutations in the Wnt, TGF-β, and PI3K pathways. Moreover, MYC-nick is elevated in colon cancer cells deleted for FBWX7, which encodes the major E3 ligase of full-length MYC frequently mutated in colorectal cancers. MYC-nick promotes the migration of colon cancer cells assayed in 3D cultures or grown as xenografts in a zebrafish metastasis model. MYC-nick accelerates migration by activating the Rho GTPase Cdc42 and inducing fascin expression. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration. PMID:27566402

  8. Nick Ransford: 'There is a job to do so let's get on with it'.

    PubMed

    Ransford, Nick; Doherty, Ruth

    2014-03-01

    Nick Ransford is a consultant in special care dentistry at Birmingham Community Healthcare NHS Trust. He has over 25 years' experience working with adults with disabilities and medical conditions across the spectrum.

  9. PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

    PubMed Central

    Grundy, Gabrielle J.; Polo, Luis M.; Zeng, Zhihong; Rulten, Stuart L.; Hoch, Nicolas C.; Paomephan, Pathompong; Xu, Yingqi; Sweet, Steve M.; Thorne, Alan W.; Oliver, Antony W.; Matthews, Steve J.; Pearl, Laurence H.; Caldecott, Keith W.

    2016-01-01

    PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break. PMID:27530147

  10. Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi

    PubMed Central

    Unciuleac, Mihaela-Carmen; Shuman, Stewart

    2015-01-01

    The proteome of the amoebo-flagellate protozoan Naegleria gruberi is rich in candidate RNA repair enzymes, including 15 putative RNA ligases, one of which, NgrRnl, is a eukaryal homolog of Deinococcus radiodurans RNA ligase, DraRnl. Here we report that purified recombinant NgrRnl seals nicked 3′-OH/5′-PO4 duplexes in which the 3′-OH strand is RNA. It does so via the “classic” ligase pathway, entailing reaction with ATP to form a covalent NgrRnl–AMP intermediate, transfer of AMP to the nick 5′-PO4, and attack of the RNA 3′-OH on the adenylylated nick to form a 3′–5′ phosphodiester. Unlike members of the four known families of ATP-dependent RNA ligases, NgrRnl lacks a carboxy-terminal appendage to its nucleotidyltransferase domain. Instead, it contains a defining amino-terminal domain that we show is important for 3′-OH/5′-PO4 nick-sealing and ligase adenylylation, but dispensable for phosphodiester synthesis at a preadenylylated nick. We propose that NgrRnl, DraRnl, and their homologs from diverse bacteria, viruses, and unicellular eukarya comprise a new “Rnl5 family” of nick-sealing ligases with a signature domain organization. PMID:25740837

  11. Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair.

    PubMed

    Davis, Luther; Maizels, Nancy

    2014-03-11

    DNA nicks are the most common form of DNA damage, and if unrepaired can give rise to genomic instability. In human cells, nicks are efficiently repaired via the single-strand break repair pathway, but relatively little is known about the fate of nicks not processed by that pathway. Here we show that homology-directed repair (HDR) at nicks occurs via a mechanism distinct from HDR at double-strand breaks (DSBs). HDR at nicks, but not DSBs, is associated with transcription and is eightfold more efficient at a nick on the transcribed strand than at a nick on the nontranscribed strand. HDR at nicks can proceed by a pathway dependent upon canonical HDR factors RAD51 and BRCA2; or by an efficient alternative pathway that uses either ssDNA or nicked dsDNA donors and that is strongly inhibited by RAD51 and BRCA2. Nicks generated by either I-AniI or the CRISPR/Cas9(D10A) nickase are repaired by the alternative HDR pathway with little accompanying mutagenic end-joining, so this pathway may be usefully applied to genome engineering. These results suggest that alternative HDR at nicks may be stimulated in physiological contexts in which canonical RAD51/BRCA2-dependent HDR is compromised or down-regulated, which occurs frequently in tumors.

  12. Appreciating Unity in Diversity: An Interview with Andrew Solomon

    ERIC Educational Resources Information Center

    Peters, Dane L.

    2014-01-01

    The theme of the AMS 2014 Annual Conference is "Unity in Diversity," a concept that also describes the work of conference keynote speaker Andrew Solomon. Solomon is a writer and lecturer on psychology and politics; winner of the National Book Award; and an activist for LGBT [lesbian, gay, bisexual, and transgender] rights, mental health,…

  13. 4. Historic American Buildings Survey L. D. Andrew, Photographer Sept. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey L. D. Andrew, Photographer Sept. 5, 1936 Photograph from photograph furnished by Miss Edith D. Johnston, Savannah, GA. VIEW OF CHANCEL IN CHRIST CHURCH BEFORE DEMOLITION photo ABOUT 1870 - Christ Church (Episcopal), 28 Bull Street, Savannah, Chatham County, GA

  14. 75 FR 32221 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers from Andrews...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ... this certification to include leased workers from Andrews International, Inc. working on-site at the... Employment and Training Administration Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers from Andrews International, Inc., Evansville, IN; Amended Certification Regarding Eligibility...

  15. General Plan Environmental Assessment for Joint Base Andrews-Naval Air Facility, Washington, Maryland

    DTIC Science & Technology

    2011-04-01

    PROPOSED ACTION ................................. 1-1  1.1  INTRODUCTION ...1 April 2011 EXECUTIVE SUMMARY PURPOSE AND NEED FOR ACTION Introduction The 2010 Andrews General Plan Update documents the existing facilities... INTRODUCTION The General Plan Update for Joint Base Andrews-Naval Air Facility, Washington, Maryland (Andrews) describes the installation’s ability to

  16. Natural and engineered nicking endonucleases—from cleavage mechanism to engineering of strand-specificity

    PubMed Central

    Chan, Siu-Hong; Stoddard, Barry L.; Xu, Shuang-yong

    2011-01-01

    Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4–8 bp palindromic sequences, have revealed a variety of mechanisms of molecular recognition and catalysis. Less well-studied are REases which cleave only one of the strands of dsDNA, creating a nick instead of a ds break. Naturally occurring nicking endonucleases (NEases) range from frequent cutters such as Nt.CviPII (^CCD; ^ denotes the cleavage site) to rare-cutting homing endonucleases (HEases) such as I-HmuI. In addition to these bona fida NEases, individual subunits of some heterodimeric Type IIS REases have recently been shown to be natural NEases. The discovery and characterization of more REases that recognize asymmetric sequences, particularly Types IIS and IIA REases, has revealed recognition and cleavage mechanisms drastically different from the canonical Type IIP mechanisms, and has allowed researchers to engineer highly strand-specific NEases. Monomeric LAGLIDADG HEases use two separate catalytic sites for cleavage. Exploitation of this characteristic has also resulted in useful nicking HEases. This review aims at providing an overview of the cleavage mechanisms of Types IIS and IIA REases and LAGLIDADG HEases, the engineering of their nicking variants, and the applications of NEases and nicking HEases. PMID:20805246

  17. DNA nicks and increased sensitivity of DNA to fluorescence in situ end labeling during functional spermiogenesis.

    PubMed

    Smith, A; Haaf, T

    1998-09-01

    Terminal transferase can be used to quantitate DNA strand breaks in situ by labeling free 3'-hydroxyl ends with exogenous nucleotides. Endogenous nicks in DNA temporally appear and disappear during functionally significant structural rearrangements of chromatin. Fluorescence in situ end labeling of mouse and rat testicular cells demonstrated that functional spermiogenesis is associated with abundant DNA nicks that occur in elongating spermatids, most likely as a result of nucleoprotein changes during terminal differentiation. Detectable DNA breaks were not observed in round spermatids and epididymal sperm.

  18. A pioneer of tropical medicine worldwide: Andrew Balfour, of Khartoum

    PubMed Central

    2013-01-01

    This is an archival account of the career of Sir Andrew Balfour in Khartoum, Sudan during the period 1902 to 1913. As the first director of the Wellcome Tropical Research Laboratories in Khartoum during the period, Andrew Balfour was tasked with establishing the laboratories and at the same time he was engaged in founding the health services in Khartoum. Balfour worked in close collaboration and support from Henry Wellcome and Reginald Wingate, the Governor General of the Sudan. The energetic and meticulous sanitary work of Balfour had a remarkable impact, with Khartoum declared mosquito-free by 1910. Establishing a research base in the laboratories was met with many challenges but eventually Balfour managed to recruit a team of dedicated researchers and to produce well-circulated publications in tropical medicine. Balfour’s work in Khartoum later lead him to a distinguished career in tropical medicine. In 1923 he was appointed the first Director of London School of Hygiene and Tropical Medicine. He was also elected President of the Royal Society of Tropical Medicine and Hygiene (1925–27). Sir Andrew Balfour, KCMG, CB, LL D (1873–1931) PMID:27493361

  19. A pioneer of tropical medicine worldwide: Andrew Balfour, of Khartoum.

    PubMed

    Adeel, Ahmed A A

    2013-01-01

    This is an archival account of the career of Sir Andrew Balfour in Khartoum, Sudan during the period 1902 to 1913. As the first director of the Wellcome Tropical Research Laboratories in Khartoum during the period, Andrew Balfour was tasked with establishing the laboratories and at the same time he was engaged in founding the health services in Khartoum. Balfour worked in close collaboration and support from Henry Wellcome and Reginald Wingate, the Governor General of the Sudan. The energetic and meticulous sanitary work of Balfour had a remarkable impact, with Khartoum declared mosquito-free by 1910. Establishing a research base in the laboratories was met with many challenges but eventually Balfour managed to recruit a team of dedicated researchers and to produce well-circulated publications in tropical medicine. Balfour's work in Khartoum later lead him to a distinguished career in tropical medicine. In 1923 he was appointed the first Director of London School of Hygiene and Tropical Medicine. He was also elected President of the Royal Society of Tropical Medicine and Hygiene (1925-27). Sir Andrew Balfour, KCMG, CB, LL D (1873 -1931).

  20. Nicking enzyme-assisted biosensor for Salmonella enteritidis detection based on fluorescence resonance energy transfer.

    PubMed

    Song, Yang; Li, Wenkai; Duan, Yingfen; Li, Zhongjie; Deng, Le

    2014-05-15

    Salmonella enteritidis (S. enteritidis) outbreaks continue to occur, and have increased public awareness of this pathogen. Nicking endonuclease Nb.BbvC I is widely used for the detection of biomolecules and displays activity for specific double-stranded DNA (dsDNA). In this study, we developed a biosensor to detect S. enteritidis based on fluorescence resonance energy transfer (FRET) using nicking enzyme and carbon nanoparticles (CNPs). Because of the quenching effect of black hole quencher 1 (BHQ 1), the CNPs do not fluoresce in the reaction system. When the target bacteria are added, the nicking enzyme recognizes and cleaves the dsDNA fabricated by the interaction between probe and target. As a result, the CNPs dissociate from BHQ 1 and emit strong fluorescence. Using the nicking enzyme, the fluorescence signals of the biosensor are greatly amplified. The biosensor exhibited a linear relationship with the concentration of S. enteritidis ranging from 10(2) to 3 × 10(3)CFU/mL in water and from 1.5 × 10(2) to 3 × 10(3)CFU/mL in milk. The present results indicate that our FRET-based detection system can be widely employed for the effective detection of pathogens.

  1. ANDREWS MOUNTAIN, MAZOURKA, AND PAIUTE ROADLESS AREAS, CALIFORNIA.

    USGS Publications Warehouse

    McKee, Edwin H.; Schmauch, Steven W.

    1984-01-01

    On the basis of a mineral survey, local areas near and within the Andrews Mountain, Mazourka, and Paiute Roadless Areas, California have probable and substantiated mineral-resource potential. The principal metallic mineral resources in these roadless areas are gold, copper, and silver with lead, zinc, and tungsten, as lesser resources. A zone of probable resource potential for talc, graphite, and marble is identified in the Mazourka Roadless Area. Metallic mineralization occurs mostly in vein deposits in silicic and carbonate metasedimentary rocks peripheral to Mesozoic plutons and locally in granitic rocks as well. There is little promise for the occurrence of fossil fuel resources in the roadless areas.

  2. Differential requirement for proliferating cell nuclear antigen in 5' and 3' nick-directed excision in human mismatch repair.

    PubMed

    Guo, Shuangli; Presnell, Steven R; Yuan, Fenghua; Zhang, Yanbin; Gu, Liya; Li, Guo-Min

    2004-04-23

    Proliferating cell nuclear antigen (PCNA) is involved in mammalian mismatch repair at a step prior to or at mismatch excision, but the molecular mechanism of this process is not fully understood. To examine the role of PCNA in mismatch-provoked and nick-directed excision, orientation-specific mismatch removal of heteroduplexes with a pre-existing nick was monitored in human nuclear extracts supplemented with the PCNA inhibitor protein p21. We show here that, whereas 3' nick-directed mismatch excision was completely inhibited by low concentrations of p21 or a p21 C-terminal fusion protein, 5' nick-directed excision was only partially blocked under the same conditions. No further reduction of the 5' excision was detected when a much higher concentration of p21 C-terminal protein was used. These results suggest the following. (i) There is a differential requirement for PCNA in 3' and 5' nick-directed excision; and (ii) 5' nick-directed excision is conducted by a manner either dependent on or independent of PCNA. Our in vitro reconstitution experiments indeed identified a 5' nick-directed excision pathway that is dependent on PCNA, hMutSalpha, and a partially purified fraction from a HeLa nuclear extract.

  3. A method for recovering strand-specific probes from nick-translated DNA fragments.

    PubMed

    Dutton, F L; Chovnick, A

    1984-07-01

    A method of preparing strand-specific probes for DNA X DNA or DNA X RNA hybridizations is described. Double-stranded DNA fragments are first isolated from any recombinant DNA clone containing the desired sequence, and then labeled in vitro by nick-translation (T. Maniatis, A. Jeffrey, and D. G. Kleid (1975) Proc. Natl. Acad. Sci. USA 72, 1184-1188; P. W. J. Rigby, M. Dieckmann, C. Rhodes, and P. Berg (1977) J. Mol. Biol. 113, 237-251). Sequences homologous to the desired strand are captured by annealing the denatured nick-translate to viral strands of an appropriate M13 clone, and recovered by elution of the resulting hybrids from a column of agarose A50M (Bio-Rad). By this method, separate probes with specificity to either strand, as well as the double-stranded probe, may conveniently be prepared from a single nick-translation reaction. Probes may be obtained which are homologous either to the full length of the cloned region or to selected portions thereof by selecting appropriate M13 clones for annealing. The probe is recovered as a population of fragments several hundred bases or less in length, which have been found ideal for saturating liquid hybridizations, and should be similarly well suited for in situ hybridizations to cytological preparations.

  4. Trapping and breaking of in vivo nicked DNA during pulsed field gel electrophoresis.

    PubMed

    Khan, Sharik R; Kuzminov, Andrei

    2013-12-15

    Pulsed field gel electrophoresis (PFGE) offers a high-resolution approach to quantify chromosomal fragmentation in bacteria, measured as percentage of chromosomal DNA entering the gel. The degree of separation in pulsed field gel (PFG) depends on the size of DNA as well as various conditions of electrophoresis such as electric field strength, time of electrophoresis, switch time, and buffer composition. Here we describe a new parameter, the structural integrity of the sample DNA itself, that influences its migration through PFGs. We show that subchromosomal fragments containing both spontaneous and DNA damage-induced nicks are prone to breakage during PFGE. Such breakage at single-strand interruptions results in artifactual decrease in molecular weight of linear DNA making accurate determination of the number of double-strand breaks difficult. Although breakage of nicked subchromosomal fragments is field strength independent, some high-molecular-weight subchromosomal fragments are also trapped within wells under the standard PFGE conditions. This trapping can be minimized by lowering the field strength and increasing the time of electrophoresis. We discuss how breakage of nicked DNA may be mechanistically linked to trapping. Our results suggest how to optimize conditions for PFGE when quantifying chromosomal fragmentation induced by DNA damage.

  5. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  6. A Meta-Analytic Review of the MacAndrew Alcoholism Scale.

    ERIC Educational Resources Information Center

    Cooper-Hakim, Amy; Viswesvaran, Chockalingam

    2002-01-01

    Using meta analysis, examined the predictive validity of scores on the MacAndrew Alcoholism Scale (C. MacAndrew, 1965). Compared results for 161 studies with results for 63 studies using cut scores. Discusses why the use of continuous measures rather than cut scores is recommended. (SLD)

  7. The Implementation of IAS 16 and IAS 41 at Andrew Peller Limited

    ERIC Educational Resources Information Center

    Lapointe-Antunes, Pascale; Moore, James

    2013-01-01

    This case asks students to play the role of Doug Grodeckie, Manager of Financial Reporting at Andrew Peller Limited (APL). Doug was asked to prepare a report analyzing Andrew Peller Limited's current tangible long-lived assets disclosures and making recommendations on how best to comply with International Accounting Standard (IAS) 16 Property,…

  8. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The...

  9. A transnational conference romance: Elsie Andrews, Hildegarde Kneeland, and the Pan-Pacific Women's Association.

    PubMed

    Laurie, Alison J

    2009-01-01

    Elsie Andrews, a feminist activist from New Zealand, met Dr. Hildegarde Kneeland, a progressive economist from the United States, at the 1934 Pan-Pacific Women's Association conference in Honolulu. Andrews wrote diaries of her attendance at conferences, and in these writes openly of her attraction and romantic feelings for Kneeland, despite her own long-term domestic partnership back in New Plymouth with Muriel Kirton. This article considers the role conference romances may have played for Andrews and others in encouraging their interest in women's organizations, in the context of literature on romantic friendships and lesbianism.

  10. Free energy analysis and mechanism of base pair stacking in nicked DNA

    PubMed Central

    Häse, Florian; Zacharias, Martin

    2016-01-01

    The equilibrium of stacked and unstacked base pairs is of central importance for all nucleic acid structure formation processes. The stacking equilibrium is influenced by intramolecular interactions between nucleosides but also by interactions with the solvent. Realistic simulations on nucleic acid structure formation and flexibility require an accurate description of the stacking geometry and stability and its sequence dependence. Free energy simulations have been conducted on a series of double stranded DNA molecules with a central strand break (nick) in one strand. The change in free energy upon unstacking was calculated for all ten possible base pair steps using umbrella sampling along a center-of-mass separation coordinate and including a comparison of different water models. Comparison to experimental studies indicates qualitative agreement of the stability order but a general overestimation of base pair stacking interactions in the simulations. A significant dependence of calculated nucleobase stacking free energies on the employed water model was observed with the tendency of stacking free energies being more accurately reproduced by more complex water models. The simulation studies also suggest a mechanism of stacking/unstacking that involves significant motions perpendicular to the reaction coordinate and indicate that the equilibrium nicked base pair step may slightly differ from regular B-DNA geometry in a sequence-dependent manner. PMID:27407106

  11. Report: Results of Technical Network Vulnerability Assessment: EPA’s Andrew W. Breidenbach Environmental Research Center

    EPA Pesticide Factsheets

    Report #10-P-0210, September 7, 2010. Vulnerability testing of EPA’s Andrew W. Breidenbach Environmental Research Center network conducted in June 2010 identified Internet Protocol addresses with numerous high-risk and medium-risk vulnerabilities.

  12. ISS Update: RATS Principal Investigator Andrew Abercromby -- 08.29.12

    NASA Video Gallery

    NASA Public Affairs Officer Brandi Dean talks to the Research And Technology Studies (RATS) Principal Investigator Andrew Abercromby in the Space Vehicle Mock-Up Facility at Johnson Space Center in...

  13. Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA)

    PubMed Central

    Chauleau, Mathieu; Shuman, Stewart

    2016-01-01

    Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO4 nicks in duplex DNA via reaction of LigA with NAD+ to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO4 to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form a 3′-5′ phosphodiester (step 3). A distinctive feature of EcoLigA is its stimulation by ammonium ion. Here we used rapid mix-quench methods to analyze the kinetic mechanism of single-turnover nick sealing by EcoLigA–AMP. For substrates with correctly base-paired 3′-OH/5′-PO4 nicks, kstep2 was fast (6.8–27 s−1) and similar to kstep3 (8.3–42 s−1). Absent ammonium, kstep2 and kstep3 were 48-fold and 16-fold slower, respectively. EcoLigA was exquisitely sensitive to 3′-OH base mispairs and 3′ N:abasic lesions, which elicited 1000- to >20000-fold decrements in kstep2. The exception was the non-canonical 3′ A:oxoG configuration, which EcoLigA accepted as correctly paired for rapid sealing. These results underscore: (i) how EcoLigA requires proper positioning of the nick 3′ nucleoside for catalysis of 5′ adenylylation; and (ii) EcoLigA's potential to embed mutations during the repair of oxidative damage. EcoLigA was relatively tolerant of 5′-phosphate base mispairs and 5′ N:abasic lesions. PMID:26857547

  14. Isothermal amplification detection of nucleic acids by a double-nicked beacon.

    PubMed

    Shi, Chao; Zhou, Meiling; Pan, Mei; Zhong, Guilin; Ma, Cuiping

    2016-03-01

    Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use.

  15. Andrew meets Rensch: sexual size dimorphism and the inverse of Rensch's rule in Andrew's toad (Bufo andrewsi).

    PubMed

    Liao, Wen Bo; Liu, Wen Chao; Merilä, Juha

    2015-02-01

    Variation in sexual size dimorphism (SSD) is a widespread phenomenon and is commonly attributed to variation in sex-specific patterns of selection. According to Rensch's rule, SSD increases with increasing body size when males are the larger sex, and decreases when females are the larger sex. Using data from 17 populations of Andrew's toad (Bufo andrewsi), we tested whether the patterns of SSD conform to Rensch's rule. Using field experiments, we also evaluated the hypothesis that sexual selection favours large male body size and that fecundity selection favours large female body size. The results revealed that the degree of SSD increased with increasing mean size in females, consistent with the inverse of Rensch's rule. Although experiments revealed evidence for a large-male mating advantage, selection for large male size was weak at best, and hence unlikely to be an important source of variation in SSD. However, fecundity selection favouring large females was evident, and likely to explain the observed inverse of Rensch's rule. After correcting male and female body size for age differences, the patterns of SSD remained the same, suggesting that the intra- and interpopulational variation in SSD is not driven by sex differences in age structure. Hence, these findings suggest that the strong fecundity selection favouring large females drives the evolution of female-biased SSD in B. andrewsi, providing an explanation for the inverse of Rensch's rule. As such, the study provides an important addition to the small body of literature that uses an intraspecific approach to demonstrate the inverse of Rensch's rule.

  16. The life of Andrew Boorde, c1490-1549.

    PubMed

    Fletcher, Patricia E S

    2002-01-01

    Physician, traveller, writer and spy, Andrew Boorde was born c1490 and became a Carthusian monk after abandoning his medical studies at Oxford. Temperamentally unsuited to the life of a religious, after 20 years at the London Charterhouse he obtained a dispensation to travel to Europe to continue his medical studies. Returning to England he began to practise medicine, treating members of the nobility and, through a meeting with Thomas Cromwell which was to influence the rest of his life, he attended the King, Henry VIII. In 1534, after a second, more extensive, tour of Europe in which he visited many medical schools and universities seeking yet more medical knowledge, he returned to the London Charterhouse which was undergoing a brutal dissolution at the hands of Thomas Cromwell. Boorde reluctantly signed the Oath of Supremacy, an act which was to haunt him for the rest of his life. He was then used by Cromwell to travel abroad again but this time as a spy to gather intelligence for the King while continuing to study medicine. Boorde finally took his MD at the University of Montpellier and was incorporated in the same degree a year later at Oxford. He then gave expression to all he had learnt by writing his legacy, four books which were published in 1547. 'A Compendyous Regyment or a Dyetary of Health' was one of the earliest treatises on the cultivation of health composed in England and stressed the importance of sanitation together with a detailed examination of diet. The 'Brevyary of Health' listed diseases alphabetically together with remedies and treatment, blending sound medical advice with religion and superstition: its companion volume was 'The Principles of Astronomy'. But Boorde's 'Fyrst Boke of the Introduction of Knowledge' was his tour-de-force; it was a comprehensive encyclopaedia of all the European countries he had visited, illustrated by woodcuts. By 1547 Boorde was settled in England, probably Master of the Hospital of St Giles-in-the-Fields in

  17. The McAndrews Leadership Lecture: February 2015, by Dr Scott Haldeman. Challenges of the Past, Challenges of the Present

    PubMed Central

    Haldeman, Scott; McAndrews, George P.; Goertz, Christine; Sportelli, Louis; Hamm, Anthony W.; Johnson, Claire

    2015-01-01

    The McAndrews Leadership Lecture was developed by the American Chiropractic Association to honor the legacy of Jerome F. McAndrews, DC, and George P. McAndrews, JD, and their contributions to the chiropractic profession. This article is a transcription of the presentation made by Dr Scott Haldeman on February 28, 2015, in Washington, DC, at the National Chiropractic Leadership Conference. PMID:26770177

  18. PARP-mediated repair, homologous recombination, and back-up non-homologous end joining-like repair of single-strand nicks.

    PubMed

    Metzger, Michael J; Stoddard, Barry L; Monnat, Raymond J

    2013-07-01

    Double-strand breaks (DSBs) in chromosomal DNA can induce both homologous recombination (HR) and non-homologous end-joining (NHEJ). Recently we showed that single-strand nicks induce HR with a significant reduction in toxicity and mutagenic effects associated with NHEJ. To further investigate the differences and similarities of DSB- and nick-induced repair, we used an integrated reporter system in human cells to measure HR and NHEJ produced by the homing endonuclease I-AniI and a designed 'nickase' variant that nicks the same target site, focusing on the PARP and HR repair pathways. PARP inhibitors, which block single-strand break repair, increased the rate of nick-induced HR up to 1.7-fold but did not affect DSB-induced HR or mutNHEJ. Additionally, expression of the PALB2 WD40 domain in trans acted as a dominant-negative inhibitor of both DSB- and nick-induced HR, sensitized cells to PARP inhibition, and revealed an alternative mutagenic repair pathway for nicks. Thus, while both DSB- and nick-induced HR use a common pathway, their substrates are differentially processed by cellular factors. These results also suggest that the synthetic lethality of PARP and BRCA may be due to repair of nicks through an error prone, NHEJ-like mechanism that is active when both PARP and HR pathways are blocked.

  19. PepFects and NickFects for the Intracellular Delivery of Nucleic Acids.

    PubMed

    Arukuusk, Piret; Pärnaste, Ly; Hällbrink, Mattias; Langel, Ülo

    2015-01-01

    Nucleic acids can be utilized in gene therapy to restore, alter, or silence gene functions. In order to reveal the biological activity nucleic acids have to reach their intracellular targets by passing through the plasma membrane, which is impermeable for these large and negatively charged molecules. Cell-penetrating peptides (CPPs) condense nucleic acids into nanoparticles using non-covalent complexation strategy and mediate their delivery into the cell, whereas the physicochemical parameters of the nanoparticles determine the interactions with the membranes, uptake mechanism, and subsequent intracellular fate. The nanoparticles are mostly internalized by endocytosis that leads to the entrapment of them in endosomal vesicles. Therefore design of new CPPs that are applicable for non-covalent complex formation strategy and harness endosomolytic properties is highly vital. Here we demonstrate that PepFects and NickFects are efficient vectors for the intracellular delivery of various nucleic acids.This chapter describes how to form CPP/pDNA nanoparticles, evaluate stable nanoparticles formation, and assess gene delivery efficacy.

  20. Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase

    PubMed Central

    Unciuleac, Mihaela-Carmen; Goldgur, Yehuda; Shuman, Stewart

    2015-01-01

    ATP-dependent RNA ligases are agents of RNA repair that join 3′-OH and 5′-PO4 RNA ends. Naegleria gruberi RNA ligase (NgrRnl) exemplifies a family of RNA nick-sealing enzymes found in bacteria, viruses, and eukarya. Crystal structures of NgrRnl at three discrete steps along the reaction pathway—covalent ligase-(lysyl-Nζ)–AMP•Mn2+ intermediate; ligase•ATP•(Mn2+)2 Michaelis complex; and ligase•Mn2+ complex—highlight a two-metal mechanism of nucleotidyl transfer, whereby (i) an enzyme-bound “catalytic” metal coordination complex lowers the pKa of the lysine nucleophile and stabilizes the transition state of the ATP α phosphate; and (ii) a second metal coordination complex bridges the β- and γ-phosphates. The NgrRnl N domain is a distinctively embellished oligonucleotide-binding (OB) fold that engages the γ-phosphate and associated metal complex and orients the pyrophosphate leaving group for in-line catalysis with stereochemical inversion at the AMP phosphate. The unique domain architecture of NgrRnl fortifies the theme that RNA ligases have evolved many times, and independently, by fusions of a shared nucleotidyltransferase domain to structurally diverse flanking modules. The mechanistic insights to lysine adenylylation gained from the NgrRnl structures are likely to apply broadly to the covalent nucleotidyltransferase superfamily of RNA ligases, DNA ligases, and RNA capping enzymes. PMID:26512110

  1. W. Horsley Gantt, Nick, and the Pavlovian Science at Phipps Clinic.

    PubMed

    Ruiz, Gabriel; Sánchez, Natividad

    2016-10-24

    William Horsley Gantt is well known as one to the principal proponents of Pavlovian methodology in the U.S. After a long stay at Ivan Petrovich Pavlov's laboratory in Leningrad from 1925 to 1929, Gantt was invited by Adolf Meyer to join the Henry Phipps Psychiatric Clinic, where he founded and directed the Pavlovian Laboratory from 1930 to 1964. Soon after his arrival at Phipps Clinic in 1931, Gantt began a Pavlovian research program that included the investigation of nervous disturbances in dogs and clinical researches with psychiatric patients. In these studies, Gantt combined a physiological method (the conditional reflexes approach), with a psychiatric problem (nervous disorders) in the context of Meyer's psychobiology that established the person or individual as unit of analysis. This fact, concentrating upon a single individual, made Gantt studies with dogs recognizable and interesting to physicians, psychologists, and psychiatrists who also worked on individuals. In this paper, we use archival materials -including correspondence, notebooks, and unpublished autobiographical material- to present a case study, that of William Horsley Gantt and his dog Nick. We will explore the reasons why Gantt' studies on nervous disturbances with this dog captured the interest of psychiatrists and clinical psychologists.

  2. Photoinduced Oxidative DNA Damage Revealed by an Agarose Gel Nicking Assay: A Biophysical Chemistry Laboratory Experiment

    NASA Astrophysics Data System (ADS)

    Shafirovich, Vladimir; Singh, Carolyn; Geacintov, Nicholas E.

    2003-11-01

    Oxidative damage of DNA molecules associated with electron-transfer reactions is an important phenomenon in living cells, which can lead to mutations and contribute to carcinogenesis and the aging processes. This article describes the design of several simple experiments to explore DNA damage initiated by photoinduced electron-transfer reactions sensitized by the acridine derivative, proflavine (PF). A supercoiled DNA agarose gel nicking assay is employed as a sensitive probe of DNA strand cleavage. A low-cost experimental and computer-interfaced imaging apparatus is described allowing for the digital recording and analysis of the gel electrophoresis results. The first experiment describes the formation of direct strand breaks in double-stranded DNA induced by photoexcitation of the intercalated PF molecules. The second experiment demonstrates that the addition of the well-known electron acceptor, methylviologen, gives rise to a significant enhancement of the photochemical DNA strand cleavage effect. This occurs by an electron transfer step to methylviologen that renders the inital photoinduced charge separation between photoexcited PF and DNA irreversible. The third experiment demonstrates that the action spectrum of the DNA photocleavage matches the absorption spectrum of DNA-bound, intercalated PF molecules, which differs from that of free PF molecules. This result demonstrates that the photoinduced DNA strand cleavage is initiated by intercalated rather than free PF molecules.

  3. Andrew's bridge system: an aesthetic and functional option for rehabilitation of compromised maxillary anterior dentition

    PubMed Central

    Tambe, Abhijit; Patil, Sanjayagouda B; Bhat, Sudhakara; Badadare, Mokshada M

    2014-01-01

    Summary A patient with several missing teeth in the anterior aesthetic region along with severe ridge defect poses a greater challenge for prosthodontic rehabilitation. In such cases treatment using fixed partial denture (FPD) may not be feasible because of the extent of edentulous span and the periodontal conditions of the abutment teeth. To present a case of multiple missing maxillary anterior teeth with class III ridge defect rehabilitated using FPD-removable partial denture. A 38-year-old female patient was successfully rehabilitated using Andrew's bridge system in the maxillary anterior region. The fixed-removable Andrew's bridge system provides a good prognosis if diagnosed and planned meticulously. PMID:25035444

  4. Analyzing after-action reports from Hurricanes Andrew and Katrina: repeated, modified, and newly created recommendations.

    PubMed

    Knox, Claire Connolly

    2013-01-01

    Thirteen years after Hurricane Andrew struck Homestead, FL, Hurricane Katrina devastated the Gulf Coast of Mississippi, Alabama, and southeastern Louisiana. Along with all its destruction, the term "catastrophic" was redefined. This article extends the literature on these hurricanes by providing a macrolevel analysis of The Governor's Disaster Planning and Response Review Committee Final Report from Hurricane Andrew and three federal after-action reports from Hurricane Katrina, as well as a cursory review of relevant literature. Results provide evidence that previous lessons have not been learned or institutionalized with many recommendations being repeated or modified. This article concludes with a discussion of these lessons, as well as new issues arising during Hurricane Katrina.

  5. Relationship of DUI recidivism to moral reasoning, sensation seeking, and MacAndrew alcoholism scores.

    PubMed

    Little, G L; Robinson, K D

    1989-12-01

    115 convicted male DUI offenders were treated with Moral Reconation Therapy during their incarceration. Postrelease recidivism status (arrests) was correlated with the pretest, posttest, and change scores on the MacAndrew Alcoholism Scale, Sensation Seeking Scale, Life-purpose scores, and Moral Reasoning scores. Analysis showed that recidivism correlated positively and significantly with the pretest scores on the MacAndrew scale and approached significance with both pre- and posttest scores on the Sensation Seeking Scale. Recidivism status correlated negatively and significantly with scores on the highest levels of moral reasoning (Scale 6 pretest and posttest and Principled Reasoning pretest).

  6. Capacities of template-type platforms in the Gulf of Mexico during hurricane Andrew

    SciTech Connect

    Bea, R.G.; Loch, K.J.; Young, P.L.

    1997-02-01

    This paper details results from nonlinear analyses of the ultimate limit state performance characteristics of four Gulf of Mexico (GOM) platforms subjected to intense loadings from hurricane Andrew. These four platforms were located to the east of the track of hurricane Andrew, and were thus in the most intense portion of the storm (Smith, 1993). The nonlinear analyses are able to replicate details of the observed behavior of the four structures. This replication is very dependent on realistic characterization of the performance characteristics of the pile foundations and on accurate information on the as is condition of the platforms before the storm.

  7. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  8. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  9. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  10. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  11. Traversing the Gap: Andrew Wright, John Hick and Critical Religious Education

    ERIC Educational Resources Information Center

    Teece, Geoff

    2005-01-01

    This paper discusses aspects of Andrew Wright's version of a liberal, critical religious education and his criticisms of some other views of modern religious education. This is attempted not by examining these "other views" as such but by concentrating on the work of John Hick. The reason for this is that Wright, like Cooling (in his…

  12. Crisis Intervention with Survivors of Natural Disaster: Lessons from Hurricane Andrew.

    ERIC Educational Resources Information Center

    Shelby, Janine S.; Tredinnick, Michael G.

    1995-01-01

    Crisis intervention has typically been conceptualized as seeking a return of clients to a state of equilibrium. Personal work experience with Hurricane Andrew survivors has led to an appreciation of the importance of several considerations. Develops a proactive approach, attempting to recognize and extend clients' preexisting strengths. Offers…

  13. 75 FR 41895 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers From Andrews...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... Workers From Andrews International, Inc., M.H. Equipment, and Kenco Logistics Services, LLC, Evansville... that workers leased from MH Equipment and Kenco Logistics Services, LLC, were employed on-site at the... from MH Equipment and Kenco Logistics Services, LLC working on-site at the Evansville, Indiana...

  14. 77 FR 2968 - Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply Comments On December 15, 2011, the Commission issued notice that Office of Energy Projects staff will hold a site visit...

  15. Because Everyone Has a Story to Tell: Interview with Andrew Wright

    ERIC Educational Resources Information Center

    Floris, Flora Debora

    2016-01-01

    This article presents an interview with Andrew Wright, a widely recognized author, illustrator, storyteller, and teacher trainer. Wright has published many ELT books, authored six "Spellbinder" graded readers (1992-1994), and a collection of short stories. As a teacher trainer, Wright worked extensively with both teachers and students in…

  16. Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-77 ESC VIEW --- Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while in the mid-deck of the Earth-orbiting Space Shuttle Endeavour. The scene was recorded with an Electronic Still Camera (ESC).

  17. Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-75 ONBOARD VIEW --- Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the flight deck of the Space Shuttle Columbia. Allen was joined by four other astronauts and an international payload specialist for more than 16 days of research aboard Columbia. The photograph was taken with a 70mm handheld camera.

  18. 56. Aerial view looking west; Andrew McLean Company Textile Mill ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. Aerial view looking west; Andrew McLean Company Textile Mill at bottom, Dundee Canal at center, Botany Worsted Mills at top - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  19. 30. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  20. 17. View looking north; Andrew McLean Company Textile Mill, Building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. View looking north; Andrew McLean Company Textile Mill, Building No. 1, at right, Botany Worsted Mills at left distance - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  1. 31. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  2. 19. View of Andrew McLean Company Textile Mill looking south; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Andrew McLean Company Textile Mill looking south; Building No. 2 at center, Botany Mills Lanolin Retrieval plant site in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  3. Amos Kendall's Role in the Election of Andrew Jackson as President of the United States, 1828.

    ERIC Educational Resources Information Center

    Nowell, Bob

    Amos Kendall's place in journalism history rests largely on his service as a journalist turned government official in the two administrations of President Andrew Jackson. Historians have claimed that Kendall was an influential journalist of the "partisan press" era, but they have provided little documentation. That documentation has been…

  4. 22. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Andrew McLean Company Textile Mill looking north; Building No. 1 at left, Building No. 4 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  5. 15. View of Andrew McLean Company Textile Mill looking northeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. View of Andrew McLean Company Textile Mill looking northeast; Building No. 1 at left, Building No. 6 at center - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  6. 14. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Andrew McLean Company Textile Mill looking north; Building No. 6 at right, Building No. 1 in background, gateposts in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  7. 20. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Andrew McLean Company Textile Mill looking southeast; Building No. 2 at left, Building No. 3 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  8. 23. View of open firststory passageway of Andrew McLean Company ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of open first-story passageway of Andrew McLean Company Textile Mill Building No. 1, looking west - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  9. 16. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. View of Andrew McLean Company Textile Mill looking southeast; Building No. 6 at center, roof of Building No. 1 at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  10. 18. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Andrew McLean Company Textile Mill looking north; Building No. 3 at center right, Building No. 2 at center distance, Dundee Canal at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  11. MGME1 processes flaps into ligatable nicks in concert with DNA polymerase γ during mtDNA replication

    PubMed Central

    Uhler, Jay P.; Thörn, Christian; Nicholls, Thomas J.; Matic, Stanka; Milenkovic, Dusanka; Gustafsson, Claes M.; Falkenberg, Maria

    2016-01-01

    Recently, MGME1 was identified as a mitochondrial DNA nuclease with preference for single-stranded DNA (ssDNA) substrates. Loss-of-function mutations in patients lead to mitochondrial disease with DNA depletion, deletions, duplications and rearrangements. Here, we assess the biochemical role of MGME1 in the processing of flap intermediates during mitochondrial DNA replication using reconstituted systems. We show that MGME1 can cleave flaps to enable efficient ligation of newly replicated DNA strands in combination with POLγ. MGME1 generates a pool of imprecisely cut products (short flaps, nicks and gaps) that are converted to ligatable nicks by POLγ through extension or excision of the 3′-end strand. This is dependent on the 3′-5′ exonuclease activity of POLγ which limits strand displacement activity and enables POLγ to back up to the nick by 3′-5′ degradation. We also demonstrate that POLγ-driven strand displacement is sufficient to generate DNA- but not RNA-flap substrates suitable for MGME1 cleavage and ligation during replication. Our findings have implications for RNA primer removal models, the 5′-end processing of nascent DNA at OriH, and DNA repair. PMID:27220468

  12. Termination sites of the in vitro nick-translation reaction on DNA that had photoreacted with psoralen.

    PubMed Central

    Piette, J G; Hearst, J E

    1983-01-01

    A double-stranded circular DNA having a single nick at a specific site has been photochemically induced to react with 4'-hydroxymethyl-4,5', 8-trimethylpsoralen (HMT) and used as a substrate for nick-translation with Escherichia coli DNA polymerase I holoenzyme. By using the dideoxy chain-terminating sequencing procedure, it was possible to map the termination sites observed on the template that had photoreacted with HMT. These sites occur at nucleotides preceding potential psoralen crosslinking sites. Analysis of DNA products synthesized on templates that had photoreacted under conditions designed to maximize psoralen monoaddition revealed that DNA polymerase I is not stopped by this lesion. Psoralen monoadducts situated on the template strand act only as kinetic attenuators, whereas psoralen monoadducts localized on the nick-translated strand have no effect on the rate of synthesis. These data suggest that psoralen crosslinks are responsible for the lethal effects of psoralen photochemistry in E. coli. Mutagenesis may be associated, however, with the repair replication of psoralen monoadducts by E. coli DNA polymerase I. Images PMID:6225123

  13. Impact of an extreme event on the sediment budget: Hurricane Andrew in the Louisiana barrier islands

    USGS Publications Warehouse

    List, Jeffrey H.; Hansen, Mark E.; Sallenger,, Asbury H.; Jaffe, Bruce E.; Edge, B.L

    1997-01-01

    This paper examines the influence of Hurricane Andrew on the sediment budget of an 80-kilometer section of the Louisiana barrier islands west of the modern Mississippi delta. Because long-term bathymetric change has been extensively studied in this area, excellent baseline data are available for evaluating the impact of Hurricane Andrew. Results show that despite the high intensity of the storm and a storm track optimally positioned to impact the study area, the storm did not have an overwhelming influence on the sediment budget when compared to the changes occurring over the previous 50 years. For the Louisiana barrier islands, a 50-year record appears to be adequate for averaging the long-term contributions of both major and minor storm events to the sediment budget.

  14. Sensitive fluorescent detection of DNA methyltransferase using nicking endonuclease-mediated multiple primers-like rolling circle amplification.

    PubMed

    Huang, Juan; Li, Xiao-Yu; Du, Yi-Chen; Zhang, Li-Na; Liu, Ke-Ke; Zhu, Li-Na; Kong, De-Ming

    2017-05-15

    Sensitive and reliable detection of DNA methyltransferase (MTase) is of great significance for both early tumor diagnosis and therapy. In this study, a simple, label-free and sensitive DNA MTase-sensing method was developed on the basis of a nicking endonuclease-mediated multiple primers-like rolling circle amplification (RCA) strategy. In this method, a dumbbell RCA template was prepared by blunt-end ligation of two molecules of hairpin DNA. In addition to the primer-binding sequence, the dumbbell template contained another three important parts: 5'-CCGG-3' sequences in double-stranded stems, nicking endonuclease recognition sites and C-rich sequences in single-stranded loops. The introduction of 5'-CCGG-3' sequences allows the dumbbell template to be destroyed by the restriction endonuclease, HpaII, but is not destroyed in the presence of the target MTase-M.SssI MTase. The introduction of nicking endonuclease recognition sites makes the M.SssI MTase-protected dumbbell template-mediated RCA proceed in a multiple primers-like exponential mode, thus providing the RCA with high amplification efficiency. The introduction of C-rich sequences may promote the folding of amplification products into a G-quadruplex structure, which is specifically recognized by the commercially available fluorescent probe thioflavin T. Improved RCA amplification efficiency and specific fluorescent recognition of RCA products provide the M.SssI MTase-sensing platform with high sensitivity. When a dumbbell template containing four nicking endonuclease sites is used, highly specific M.SssI MTase activity detection can be achieved in the range of 0.008-50U/mL with a detection limit as low as 0.0011U/mL. Simple experimental operation and mix-and-detection fluorescent sensing mode ensures that M.SssI MTase quantitation works well in a real-time RCA mode, thus further simplifying the sensing performance and making high throughput detection possible. The proposed MTase-sensing strategy was also

  15. 21. View of Andrew McLean Company Textile Mill looking west; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Andrew McLean Company Textile Mill looking west; Building No. 4 at left, Building No. 1 at left center, Building No. 3 at center, Building No. 2 at right center, Building No. 5 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  16. Environmental Assessment of the Privatization of Military Family Housing at Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2007-05-01

    congressional legislation. b. Ensures that eligible military members and their families have access to quality, attractive , and affordable housing...average annual temperature at Andrews AFB is 56 degrees Fahrenheit (ºF), the mean annual precipitation is 42.5 inches, the mean average snowfall is...surface water turbidity, which can raise water temperature and impede photosynthetic processes. Sediment runoff into surface water bodies also

  17. Environmental Assessment of Communications Support Facilities Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2004-12-01

    Anacostia River on the west and the Patuxent River on the east. Land surface elevations on Andrews AFB vary from approximately 215 feet above mean sea...within three significantly diverse watersheds: the Potomac River , Anacostia River , and Patuxent River . These watersheds drain 2.3 17 square miles of...George’s County, while 132,000 acres drain to the Anacostia River (USA F 200 I). The majority of the base lies within the Potomac Ri ver Watershed

  18. Andrew Wyeth and N.C. Wyeth: a psychodynamic perspective on father and son.

    PubMed

    Shaw, Jon A

    2013-01-01

    This paper examines the relationship between two extraordinary artists, father and son--N.C. Wyeth (1882-1945) and Andrew Wyeth (1917-2009)--and their art. N.C. Wyeth, the father, the most famous illustrator of his day, painted scenes full of drama and action, often of men engaged in violent life and death struggles. N.C. was unable to separate from his powerful mother and yearned for his iconic father. He thought himself an artistic failure and dedicated himself to raising his children to be geniuses. The youngest son, Andrew Wyeth, who lived a "secret life," painted scenes often characterized by pathos: bleak and barren landscapes, leaden skies, tire tracks, gray framed houses, desiccated fields, and circling buzzards. In the father-son relationship, we often seen three themes perpetuated developmentally: (1) the son's identification with the innermost conflicts of his father; (2) the yearning for the iconic father of his youth; and (3) a continuation and disavowal of his father's life. These themes are played out in the relationship between Andrew Wyeth and his father.

  19. 78 FR 6173 - Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF TRANSPORTATION Surface Transportation Board Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen... Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and Richard Kosiba...

  20. Environmental Assessment of Modifications to Building 1535 and Demolition of 3306 at Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2003-09-01

    100 feet except for moderately steep stream banks. Andrews AFB is located in a level plateau between the Anacostia River on the Andrews AFB, MD...surface water quality also because of the potential to introduce sediments and other contaminants into lakes, rivers , and streams. Storm water...watersheds are the Potomac River , Anacostia , and Patuxent. These watersheds drain 2,317 square miles of the east-central portion of the Chesapeake

  1. Sensitive and specific colorimetric DNA detection by invasive reaction coupled with nicking endonuclease-assisted nanoparticles amplification.

    PubMed

    Zou, Bingjie; Cao, Xiaomei; Wu, Haiping; Song, Qinxin; Wang, Jianping; Kajiyama, Tomoharu; Kambara, Hideki; Zhou, Guohua

    2015-04-15

    Colorimetric DNA detection is preferable to methods in clinical molecular diagnostics, because no expensive equipment is required. Although many gold nanoparticle-based colorimetric DNA detection strategies have been developed to analyze DNA sequences of interest, few of them can detect somatic mutations due to their insufficient specificity. In this study, we proposed a colorimetric DNA detection method by coupling invasive reaction with nicking endonuclease-assisted nanoparticles amplification (IR-NEANA). A target DNA firstly produces many flaps by invasive reaction. Then the flaps are converted to targets of nicking reaction-assisted nanoparticles amplification by ligation reaction to produce the color change of AuNPs, which can be observed by naked eyes. The detection limit of IR-NEANA was determined as 1pM. Most importantly, the specificity of the method is high enough to pick up as low as 1% mutant from a large amount of wild-type DNA backgrounds. The EGFR gene mutated at c.2573 T>G in 9 tissue samples from non-small cell lung cancer patients were successfully detected by using IR-NEANA, suggesting that our proposed method can be used to detect somatic mutations in biological samples.

  2. Nicking endonuclease-assisted recycling of target-aptamer complex for sensitive electrochemical detection of adenosine triphosphate.

    PubMed

    Hu, Tianxing; Wen, Wei; Zhang, Xiuhua; Wang, Shengfu

    2016-02-21

    An electrochemical biosensor was developed for the detection of adenosine triphosphate (ATP) based on target-induced conformation switching and nicking endonuclease (NEase)-assisted signal amplification. The electrochemical biosensor was constructed by base pairing and target recognition. After capture DNA hybridized with the gold electrode, a significant current of Methylene Blue (MB) was obtained by differential pulse voltammetry. In the presence of ATP, the hairpin DNA formed a G-quadruplex structure due to the specific recognition between hairpin DNA and ATP. Then the exposed part of the target-aptamer complex hybridized with the 3'-terminus of capture DNA to form a specific nicking site for Nb.BbvCI, which led to NEase-assisted target-aptamer complex recycling. The released target-aptamer complex hybridized with the remaining capture DNA. Nb.BbvCI-assisted target-aptamer complex recycling caused the continuous cleavage of capture DNA with MB at its 5'-terminus, resulting in release of a certain amount of DNA fragment labeled with MB. Then the current value decreased significantly. The reduced current showed a linear range from 10 nM to 1 μM with a limit of detection as low as 3.4 nM. Furthermore, the proposed strategy can be used for the detection of similar substances.

  3. Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus.

    PubMed

    Rouhibakhsh, A; Choudhury, N R; Mukherjee, S K; Malathi, V G

    2012-04-01

    Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Blackgram 3:1991] MYMIV-[IN:ND:Bg3:91] and cowpea isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Cowpea7:1998] MYMIV-[IN:ND:Cp7:98], respectively, were over expressed in Escherichia coli in fusion with maltose binding protein (MBP). The recombinant protein did not show efficient binding to DNA. However, both MBP-BgAV2 and MBP-CpAV2 proteins modulated nicking and ATPase activity of replication initiation protein (Rep). Even low concentration, 20 ng of MBP-BgAV2 and MBP-CpAV2 could bring 20 folds increase in nicking activity of Rep. Similarly in the presence of AV2 protein, two to three fold increase in ATPase activity was observed. It is hypothesized that AV2 protein may play a role of accessory protein modulating Rep activities.

  4. Research on an in-situ vision inspection system of the nick depth of easy open end

    NASA Astrophysics Data System (ADS)

    Huang, Liu; Xu, Yong-ming; Zheng, Ying-jun; Guo, Tian-tai; Yu, Gui-ying

    2011-08-01

    The size and shape of the nick of easy open end are key parameters in ensuring high packaging quality, but the present detection methods are still rather primitive. To solve this problem and realize automatic detection of easy open end in industrial production sites, an in-situ vision inspection system based on light-section principle is developed to measure the nick depth quickly and accurately, which utilizes self-developed image acquisition and processing software and uses an automatic blocking and threshold segmentation algorithm based on projection method. Experimental results show that the inspection system has realized the positioning accuracy of +/-1mm in X and Y directions, and the depth of field in the system is 700μm. The vibration characteristics of the system is tested by using shaking table with vibration frequency of 6.7Hz and amplitude of 360μm to simulate production environment of easy open end, which verifies that the system can work in similar production environments.

  5. Mus81-Mms4 Functions as a Single Heterodimer To Cleave Nicked Intermediates in Recombinational DNA Repair

    PubMed Central

    Schwartz, Erin K.; Wright, William D.; Ehmsen, Kirk T.; Evans, James E.; Stahlberg, Henning

    2012-01-01

    The formation of crossovers is a fundamental genetic process. The XPF-family endonuclease Mus81-Mms4 (Eme1) contributes significantly to crossing over in eukaryotes. A key question is whether Mus81-Mms4 can process Holliday junctions that contain four uninterrupted strands. Holliday junction cleavage requires the coordination of two active sites, necessitating the assembly of two Mus81-Mms4 heterodimers. Contrary to this expectation, we show that Saccharomyces cerevisiae Mus81-Mms4 exists as a single heterodimer both in solution and when bound to DNA substrates in vitro. Consistently, immunoprecipitation experiments demonstrate that Mus81-Mms4 does not multimerize in vivo. Moreover, chromatin-bound Mus81-Mms4 does not detectably form higher-order multimers. We show that Cdc5 kinase activates Mus81-Mms4 nuclease activity on 3′ flaps and Holliday junctions in vitro but that activation does not induce a preference for Holliday junctions and does not induce multimerization of the Mus81-Mms4 heterodimer. These data support a model in which Mus81-Mms4 cleaves nicked recombination intermediates such as displacement loops (D-loops), nicked Holliday junctions, or 3′ flaps but not intact Holliday junctions with four uninterrupted strands. We infer that Mus81-dependent crossing over occurs in a noncanonical manner that does not involve the coordinated cleavage of classic Holliday junctions. PMID:22645308

  6. Management of Oro-Nasal Fistula Using Andrew's Bridge: A Case Report.

    PubMed

    Harish, P V; Bhojaraju, Nandakishore; Sowmya, G R; Gangaiah, Makam

    2014-09-01

    Oro-nasal fistula is the most common complication following the surgical closure of the cleft palate. Retention is the paramount factor in the successful prosthodontic habilitation of cleft palate patients. Various precision attachments have provided us with the opportunity to make the prosthesis fixed removable type; giving a double advantage to the patient i.e. comfort through fixed type and easy maintenance through removal type. This case report describes a case of oro-nasal fistula habilitated with an obturator attached to Andrew's bridge, which had good retention and esthetics.

  7. Cognition About the Creative Process – Interview With Dr Andrew P. Allen

    PubMed Central

    Allen, Andrew P.; Loughnane, Lynda

    2016-01-01

    What is the relationship between the creative process and cognition and perception? Lynda Loughnane, a master’s student in Art and Process in Crawford College of Art and Design, Cork, Ireland interviewed Dr Andrew P. Allen about the subject. Areas covered include mindfulness, Type 1 and Type 2 thinking, stage theories of creativity, engagement with the art process and the artwork, phenomenology and consciousness with and without self report. The interview was constructed to cover a wide range of subject matter, so as to gather as much information as possible in layman's language about the cognitive process in relation to creativity and interaction with art. PMID:27872674

  8. Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks

    PubMed Central

    Renouf, Benjamin; Piganeau, Marion; Ghezraoui, Hind; Jasin, Maria; Brunet, Erika

    2015-01-01

    Recurrent chromosomal translocations are found in numerous of tumor types, often leading to the formation and expression of fusion genes with oncogenic potential. Creating chromosomal translocations at the relevant endogenous loci, rather than just ectopically expressing the fusion genes, opens new possibilities for better characterizing molecular mechanisms driving tumor formation. In this chapter, we describe methods to create cancer translocations in human cells. DSBs or paired nicks generated by either wild-type Cas9 or the Cas9 nickase, respectively, are used to induce translocations at the relevant loci. Using different PCR-based methods, we also explain how to quantify translocation frequency and to analyze breakpoint junctions in the cells of interest. In addition, PCR detection of translocations is used as a very sensitive method to detect off-target effects, which has general utility. PMID:25398344

  9. Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks.

    PubMed

    Renouf, Benjamin; Piganeau, Marion; Ghezraoui, Hind; Jasin, Maria; Brunet, Erika

    2014-01-01

    Recurrent chromosomal translocations are found in numerous tumor types, often leading to the formation and expression of fusion genes with oncogenic potential. Creating chromosomal translocations at the relevant endogenous loci, rather than ectopically expressing the fusion genes, opens new possibilities for better characterizing molecular mechanisms driving tumor formation. In this chapter, we describe methods to create cancer translocations in human cells. DSBs or paired nicks generated by either wild-type Cas9 or the Cas9 nickase, respectively, are used to induce translocations at the relevant loci. Using different PCR-based methods, we also explain how to quantify translocation frequency and to analyze breakpoint junctions in the cells of interest. In addition, PCR detection of translocations is used as a very sensitive method to detect off-target effects, which has general utility.

  10. Ecological evaluation of proposed dredged material from St. Andrew Bay, Florida

    SciTech Connect

    Mayhew, H.L.; Word, J.Q.; Kohn, N.P.; Pinza, M.R.; Karle, L.M.; Ward, J.A.

    1993-10-01

    The US Army Corps of Engineers (USACE), Mobile District, requested that the Battelle/Marine Sciences Laboratory (MSL) conduct field sampling and chemical and biological testing to determine the suitability of potential dredged material for open ocean disposal. Sediment from St. Andrew Bay was chemically characterized and evaluated for biological toxicity and bioaccumulation of contaminants. The Tier III guidance for ocean disposal testing requires tests of water column effects (following dredged material disposal), deposited sediment toxicity, and bioaccumulation of contaminants from deposited sediment (dredged material). To meet these requirements, the MSL conducted suspended-particulate-phase (SPP) toxicity tests, solid-phase toxicity tests, and bioaccumulation testing on sediment representing potential dredged material from Panama City Harbor. Physical and chemical characterization of sediment to support toxicity and bioaccumulation results was also conducted on both the test and reference sediments. The MSL collected sediment samples from five sites in St. Andrew Bay and one reference site near Lands End Peninsula. The five test sediments and the reference sediment were analyzed for physical and chemical sediment characteristics, SPP chemical contaminants, solid-phase toxicity, SPP toxicity, and bioaccumulation of contaminants.

  11. Delayed tree mortality in the Atchafalaya Basin of Southern Louisiana following Hurricane Andrew

    USGS Publications Warehouse

    Keeland, B.D.; Gorham, L.E.

    2009-01-01

    Hurricanes can damage trees in forested wetlands, and the potential for mortality related to these storms exists due to the effects of tree damage over time. In August 1992, Hurricane Andrew passed through the forested wetlands of southern Louisiana with winds in excess of 225 kph. Although more than 78 of the basal area was destroyed in some areas, most trees greater than 2.5 cm dbh were alive and resprouting prolifically the following year (98.8). Survival of most tree species was similarly high two years after the hurricane, but mortality rates of some species increased dramatically. For example, Populus heterophylla (swamp cottonwood) mortality increased from 7.8 to 59.2 (n 76) and Salix interior (sandbar willow) mortality increased from 4.5 to 57.1 (n 21). Stem sprouts on many up-rooted hardwood trees of other species were still alive in 1998, 6 years after the hurricane. Due to the understory tree species composition, regeneration, and high levels of resprouting, there was little change in species composition or perhaps a slight shift toward more shade and flood tolerant species six years following the hurricane event. Triadica sebifera (Chinese tallow) was found on some of the sites heavily disturbed by Hurricane Andrew, and may proliferate at the expense of native tree species. ?? 2009 The Society of Wetland Scientists.

  12. Development of pile foundation bias factors using observed behavior of platforms during Hurricane Andrew

    SciTech Connect

    Aggarwal, R.K.; Litton, R.W.; Cornell, C.A.; Tang, W.H.; Chen, J.H.; Murff, J.D.

    1996-12-31

    The performance of more than 3,000 offshore platforms in the Gulf of Mexico was observed during the passage of Hurricane Andrew in August 1992. This event provided an opportunity to test the procedures used for platform analysis and design. A global bias was inferred for overall platform capacity and loads in the Andrew Joint Industry Project (JIP) Phase 1. It was predicted that the pile foundations of several platforms should have failed, but did not. These results indicated that the biases specific to foundation failure modes may be higher than those of jacket failure modes. The biases in predictions of foundation failure modes were therefore investigated further in this study. The work included capacity analysis and calibration of predictions with the observed behavior for 3 jacket platforms and 3 caissons using Bayesian updating. Bias factors for two foundation failure modes, lateral shear and overturning, were determined for each structure. Foundation capacity estimates using conventional methods were found to be conservatively biased overall.

  13. James Gregory, the University observatory and the early acquisition of scientific instruments at the University of St Andrews

    PubMed Central

    Rawson, Helen C.

    2015-01-01

    James Gregory, inventor of the reflecting telescope and Fellow of the Royal Society, was the first Regius Professor of Mathematics of the University of St Andrews, 1668–74. He attempted to establish in St Andrews what would, if completed, have been the first purpose-built observatory in the British Isles. He travelled to London in 1673 to purchase instruments for equipping the observatory and improving the teaching and study of natural philosophy and mathematics in the university, seeking the advice of John Flamsteed, later the first Astronomer Royal. This paper considers the observatory initiative and the early acquisition of instruments at the University of St Andrews, with reference to Gregory's correspondence, inventories made ca. 1699–ca. 1718 and extant instruments themselves, some of which predate Gregory's time. It examines the structure and fate of the university observatory, the legacy of Gregory's teaching and endeavours, and the meridian line laid down in 1748 in the University Library.

  14. John Steinbeck: "The Pearl," Adapted by Warren Frost and Dramatized for the Kennedy Center by Nick Olcott. Cue Sheet for Students.

    ERIC Educational Resources Information Center

    Carr, John C.

    This performance guide is designed for teachers to use with students before and after a performance of "The Pearl" by John Steinbeck, adapted by Warren Frost and dramatized for the Kennedy Center by Nick Olcott. It is in the form of a Director's Notebook--a scrapbook/journal of clippings, memos, lists, illustrations, notes, and other…

  15. The post-disaster negative health legacy: pregnancy outcomes in Louisiana after Hurricane Andrew.

    PubMed

    Antipova, Anzhelika; Curtis, Andrew

    2015-10-01

    Disasters and displacement increasingly affect and challenge urban settings. How do pregnant women fare in the aftermath of a major disaster? This paper investigates the effect of pregnancies in disaster situations. The study tests a hypothesis that pregnant women residing in hurricane-prone areas suffer higher health risks. The setting is Louisiana in the Gulf Coast, United States, a state that continually experiences hurricane impacts. The time period for the analysis is three years following the landfall of Hurricane Andrew in 1992. We analysed low birth weight and preterm deliveries before and after landfall, as a whole and by race. Findings support an association between hazards and health of a community and indicate that pregnant women in the affected area, irrespective of race, are more likely to experience preterm deliveries compared to pre-event births. Results suggest there is a negative health legacy impact in Louisiana as a result of hurricane landfall.

  16. Business closure and relocation: a comparative analysis of the Loma Prieta earthquake and Hurricane Andrew.

    PubMed

    Wasileski, Gabriela; Rodríguez, Havidán; Diaz, Walter

    2011-01-01

    The occurrence of a number of large-scale disasters or catastrophes in recent years, including the Indian Ocean tsunami (2004), the Kashmir earthquake (2005), Hurricane Katrina (2005) and Hurricane Ike (2008), have raised our awareness regarding the devastating effects of disasters on human populations and the importance of developing mitigation and preparedness strategies to limit the consequences of such events. However, there is still a dearth of social science research focusing on the socio-economic impact of disasters on businesses in the United States. This paper contributes to this research literature by focusing on the impact of disasters on business closure and relocation through the use of multivariate logistic regression models, specifically focusing on the Loma Prieta earthquake (1989) and Hurricane Andrew (1992). Using a multivariate model, we examine how physical damage to the infrastructure, lifeline disruption and business characteristics, among others, impact business closure and relocation following major disasters.

  17. STS-89 M.S. Andrew Thomas, poses the day before launch

    NASA Technical Reports Server (NTRS)

    1998-01-01

    STS-89 Mission Specialist Andrew Thomas, Ph.D., poses at KSC's Launch Pad 39A wearing a miniature koala bear on the day before the scheduled launch of Space Shuttle Endeavour that will carry him up to the Russian Space Station Mir. Final preparations are under way toward liftoff on Jan. 22 on the eighth mission to dock with Mir. After docking, Dr. Thomas will transfer to the space station, succeeding David Wolf, M.D., who will return to Earth aboard Endeavour. Dr. Thomas, who was born and educated in South Australia, will live and work on Mir until June. STS-89 is scheduled for liftoff at 9:48 p.m. EST.

  18. Children's predisaster functioning as a predictor of posttraumatic stress following Hurricane Andrew.

    PubMed

    La Greca, A M; Silverman, W K; Wasserstein, S B

    1998-12-01

    This study examined (a) children's predisaster behavioral and academic functioning as a predictor of posttraumatic stress (PTS) following Hurricane Andrew and (b) whether children who were exposed to the disaster would display a worsening of prior functioning. Fifteen months before the disaster, 92 4th through 6th graders provided self-reports of anxiety; peers and teachers rated behavior problems (anxiety, inattention, and conduct) and academic skills. Measures were repeated 3 months postdisaster; children also reported PTS symptoms and hurricane-related experiences (i.e., exposure). PTS symptoms were again assessed 7 months postdisaster. At 3 months postdisaster, children's exposure to the disaster, as well as predisaster ratings of anxiety, inattention, and academic skills, predicted PTS symptoms. By 7 months, only exposure, African American ethnicity, and predisaster anxiety predicted PTS. Prior anxiety levels also worsened as a result of exposure to the disaster. The findings have implications for identifying and treating children at risk for stress reactions following a catastrophic disaster.

  19. Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata).

    PubMed

    Johnston, S D; López-Fernández, C; Gosálbez, A; Holt, W V; Gosálvez, J

    2009-01-01

    Prototherian spermatozoa are unique amongst the Mammalia in terms of their filiform morphology, tandem arrangement of chromosomes and formation of sperm bundles. In the present study, we provide observations of echidna spermatozoa and note that the superstructure of the bundle is engineered around the shape of the individual sperm head and that this in turn may be a consequence of the unusual circumferential and helicoidal condensation of the DNA during spermiogenesis. Frozen-thawed ejaculated echidna spermatozoa were incubated and examined for the presence of non-typical DNA conformation by means of in situ labelling of DNA breaks using Klenow polymerase and via alkaline single-cell comet assays for detection of fragmented DNA. Both techniques successfully revealed the presence of what appeared to be directional DNA nicking, co-localised with the presence of highly sensitive alkali sites along the length of the sperm nucleus. It was not possible to define whether these alternative DNA configurations were associated with a failure of the sperm nucleus to condense appropriately during spermiogenesis or were evidence of DNA fragmentation following post-thaw incubation or a sequential structural chromatin rearrangement necessary for fertilisation.

  20. Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks

    NASA Astrophysics Data System (ADS)

    Corbett, Sybilla Louise; Sharma, Rajan; Davies, Alexander Giles; Wälti, Christoph

    2017-01-01

    The use of DNA as a structural material for nanometre-scale construction has grown extensively over the last decades. The development of more advanced DNA-based materials would benefit from a modular approach enabling the direct assembly of additional elements onto nanostructures after fabrication. RecA-based nucleoprotein filaments encapsulating short ssDNA have been demonstrated as a tool for highly efficient and fully programmable post-hoc patterning of duplex DNA scaffold. However, the underlying assembly process is not fully understood, in particular when patterning complex DNA topologies. Here, we report the effect of basepair-mismatched regions and single-strand nicks in the double-stranded DNA scaffold on the yield of RecA-based assembly. Significant increases in assembly yield are observed upon the introduction of unpaired basepairs directly adjacent to the assembly region. However, when the unpaired regions were introduced further from the assembly site the assembly yield initially decreased as the length of the unpaired region was increased. These results suggest that an unpaired region acts as a kinetic trap for RecA-based nucleoprotein filaments, impeding the assembly mechanism. Conversely, when the unpaired region is located directly adjacent to the assembly site, it leads to an increase in efficiency of RecA patterning owing to increased breathing of the assembly site.

  1. Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks

    PubMed Central

    Corbett, Sybilla Louise; Sharma, Rajan; Davies, Alexander Giles; Wälti, Christoph

    2017-01-01

    The use of DNA as a structural material for nanometre-scale construction has grown extensively over the last decades. The development of more advanced DNA-based materials would benefit from a modular approach enabling the direct assembly of additional elements onto nanostructures after fabrication. RecA-based nucleoprotein filaments encapsulating short ssDNA have been demonstrated as a tool for highly efficient and fully programmable post-hoc patterning of duplex DNA scaffold. However, the underlying assembly process is not fully understood, in particular when patterning complex DNA topologies. Here, we report the effect of basepair-mismatched regions and single-strand nicks in the double-stranded DNA scaffold on the yield of RecA-based assembly. Significant increases in assembly yield are observed upon the introduction of unpaired basepairs directly adjacent to the assembly region. However, when the unpaired regions were introduced further from the assembly site the assembly yield initially decreased as the length of the unpaired region was increased. These results suggest that an unpaired region acts as a kinetic trap for RecA-based nucleoprotein filaments, impeding the assembly mechanism. Conversely, when the unpaired region is located directly adjacent to the assembly site, it leads to an increase in efficiency of RecA patterning owing to increased breathing of the assembly site. PMID:28112216

  2. Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks.

    PubMed

    Corbett, Sybilla Louise; Sharma, Rajan; Davies, Alexander Giles; Wälti, Christoph

    2017-01-23

    The use of DNA as a structural material for nanometre-scale construction has grown extensively over the last decades. The development of more advanced DNA-based materials would benefit from a modular approach enabling the direct assembly of additional elements onto nanostructures after fabrication. RecA-based nucleoprotein filaments encapsulating short ssDNA have been demonstrated as a tool for highly efficient and fully programmable post-hoc patterning of duplex DNA scaffold. However, the underlying assembly process is not fully understood, in particular when patterning complex DNA topologies. Here, we report the effect of basepair-mismatched regions and single-strand nicks in the double-stranded DNA scaffold on the yield of RecA-based assembly. Significant increases in assembly yield are observed upon the introduction of unpaired basepairs directly adjacent to the assembly region. However, when the unpaired regions were introduced further from the assembly site the assembly yield initially decreased as the length of the unpaired region was increased. These results suggest that an unpaired region acts as a kinetic trap for RecA-based nucleoprotein filaments, impeding the assembly mechanism. Conversely, when the unpaired region is located directly adjacent to the assembly site, it leads to an increase in efficiency of RecA patterning owing to increased breathing of the assembly site.

  3. Ultrasensitive DNA detection by cycle isothermal amplification based on nicking endonuclease and its application to logic gates.

    PubMed

    Li, Xuemei; Ding, Tianrong; Sun, Li; Mao, Changming

    2011-12-15

    In recent years, an intense interest has grown in the DNA logic gates having high potential for computation at literally the "nano-size" level. A limitation of traditional DNA logic gates is that each target strand hybridizes with only a single copy of the probe. This 1:1 hybridization radio limits the gain of the approach and thus its sensitivity. The exponential amplification of nucleic acids has become a core technology in medical diagnostics and has been widely used for the construction of DNA sensor, DNA nanomachine and DNA sequencing. It would be of great interest to develop DNA-based logic systems with exponential amplification for the output signal. In the present study, a series of three-input DNA logic gates with the cycle isothermal amplification based on nicking endonuclease (NEase) are designed. Very low concentrations of the analytes were sufficient to initiate an autocatalytic cascade, achieving a significant improvement of the detection limit, 100-fold improvement compared to the non-autocatalytic system. This was achieved by engineering a simple and flexible biological circuit designed to initiate a cascade of events to detect and amplify a specific DNA sequence. This procedure has the potential to greatly simplify the logic operation because amplification can be performed in "one-pot".

  4. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.760 Naval Support Activity Panama City and... permission from the Officer in Charge, Naval Support Activity Panama City, Panama City Beach, Florida, or...

  5. Religious Literacy or Spiritual Awareness? Comparative Critique of Andrew Wright's and David Hay's Approaches to Spiritual Education

    ERIC Educational Resources Information Center

    Filipsone, Anta

    2009-01-01

    On the basis of a comparison of the educational approaches of Andrew Wright and David Hay this paper illustrates the persisting problem of dichotomising cognitive and trans-cognitive aspects of spiritual development and education. Even though both Wright and Hay speak of the same topic--spirituality and spiritual education--they define these terms…

  6. The Berrien County Math and Science Center at Andrews University: A University and Local High Schools Consortium Model.

    ERIC Educational Resources Information Center

    Atkins, Gordon; Adkin, Sally

    1995-01-01

    Describes a program at Andrews University developed along with the Berrien County Intermediate School District that utilizes college faculty to provide instruction to talented and motivated high school students. Explains logistics (curriculum design, administrative arrangement, and financial aspects) of the program, challenges, and benefits.…

  7. Should Community College Be Free? Forum. "Education Next" Talks with Sara Goldrick-Rab and Andrew P. Kelly

    ERIC Educational Resources Information Center

    Goldrick-Rab, Sara; Kelly, Andrew P.

    2016-01-01

    In this article, "Education Next" talks with Sara Goldrick-Rab and Andrew Kelly. President Obama's proposal for tuition-free community college, seems to have laid down a marker for the Democratic Party. Vermont senator Bernie Sanders is touting his plan for free four-year public college on the primary trail; Massachusetts senator…

  8. [The Andrew Heiskell Library for the Blind and Physically Handicapped.] A Library with a Difference. Projects and Experiments.

    ERIC Educational Resources Information Center

    Jarkon, Joe; Fitzpatrick, Vicki, Ed.

    This publication describes the Andrew Heiskell Library for the Blind and Physically Handicapped, a regional library for the National Library Service (NLS) example of the creative use of physical space and innovative technology. The publication focuses on the materials-handling system designed for the new facility, including system design…

  9. Simple method for pretreatment of tissue sections for the detection of apoptosis by in situ end-labelling and in situ nick translation

    PubMed Central

    Panchalingam, S; Reynolds, G M; Lammas, D A; Rowlands, D C; Kumararatne, D S

    1996-01-01

    Aims—To overcome the problems associated with proteolytic pretreatment of tissue sections for the detection of apoptosis. Methods—Formalin fixed, paraffin wax embedded tissue sections of reactive lymph nodes and biopsy specimens of Burkitt lymphoma were pretreated by pressure cooking for the detection of apoptosis using the in situ end-labelling and in situ nick translation methods. Results—The results achieved with the in situ end-labelling and nick translations methods were compared with those obtained using a novel anti-apoptosis specific protein (ASP) antibody. The staining patterns generated using the three methods were similar and consistent, although the ASP antibody seemed to be more sensitive and detected higher numbers of apoptotic cells within sections. Conclusions—Pressure cooking is advocated as an alternative method to proteolytic enzyme digestion for pretreating paraffin wax sections. It is reliable, inexpensive, reduces the need to optimise pretreatment variables for different tissues, and permits double immunostaining of sections. Images PMID:16696088

  10. Ultrasensitive and selective electrochemical biosensor for detection of mercury (II) ions by nicking endonuclease-assisted target recycling and hybridization chain reaction signal amplification.

    PubMed

    Hong, Minqiang; Wang, Mengyan; Wang, Jing; Xu, Xueqin; Lin, Zhenyu

    2017-02-22

    In this paper, a novel and signal-on electrochemical biosensor based on Hg(2+)- triggered nicking endonuclease-assisted target recycling and hybridization chain reaction (HCR) amplification tactics was developed for sensitive and selective detection of Hg(2+). The hairpin-shaped capture probe A (PA) contained a specific sequence which was recognized by nicking endonuclease (NEase). In the presence of Hg(2+), probe B (PB) hybridized with PA to form stand-up duplex DNA strands via the Hg(2+) mediated thymine-Hg(2+)-thymine (T-Hg(2+)-T) structure, which automatically triggered NEase to selectively digest duplex region from the recognition sites, spontaneously dissociating PB and Hg(2+) and leaving the remnant initiators. The released PB and Hg(2+) could be reused to initiate the next cycle and more initiators were generated. The long nicked double helices were formed through HCR event, which was triggered by the initiators and two hairpin-shaped signal probes labeled with methylene blue, resulting in a significant signal increase. Under optimum conditions, the resultant biosensor showed the high sensitivity and selectivity for the detection of Hg(2+) in a linear range from 10 pM to 50nM (R(2)=0.9990), and a detection limit as low as 1.6 pM (S/N=3). Moreover, the proposed biosensor was successfully applied in the detection of Hg(2+) in environment water samples with satisfactory results.

  11. Genomewide scan for adaptive differentiation along altitudinal gradient in the Andrew's toad Bufo andrewsi.

    PubMed

    Guo, Baocheng; Lu, Di; Liao, Wen Bo; Merilä, Juha

    2016-08-01

    Recent studies of humans, dogs and rodents have started to discover the genetic underpinnings of high altitude adaptations, yet amphibians have received little attention in this respect. To identify possible signatures of adaptation to altitude, we performed a genome scan of 15 557 single nucleotide polymorphisms (SNPs) obtained with restriction site-associated DNA sequencing of pooled samples from 11 populations of Andrew's toad (Bufo andrewsi) from the edge of the Tibetan Plateau, spanning an altitudinal gradient from 1690 to 2768 m.a.s.l. We discovered significant geographic differentiation among all sites, with an average FST   = 0.023 across all SNPs. Apart from clear patterns of isolation by distance, we discovered numerous outlier SNPs showing strong associations with variation in altitude (1394 SNPs), average annual temperature (1859 SNPs) or both (1051 SNPs). Levels and patterns of genetic differentiation in these SNPs were consistent with the hypothesis that they have been subject to directional selection and reflect adaptation to altitudinal variation among the study sites. Genes with footprints of selection were significantly enriched in binding and metabolic processes. Several genes potentially related to high altitude adaptation were identified, although the identity and functional significance of most genomic targets of selection remain unknown. In general, the results provide genomic support for results of earlier common garden and low coverage genetic studies that have uncovered substantial adaptive differentiation along altitudinal and latitudinal gradients in amphibians.

  12. Storm-tide elevations produced by Hurricane Andrew along the southern Florida coasts, August 24, 1992

    USGS Publications Warehouse

    Murray, Mitchell H.

    1994-01-01

    On August 24, 1992, Hurricane Andrew crossed southern peninsular Florida. The combined effects of storm surge from the hurricane and astronomical tide, referred to as storm tide, caused flooding over a large part of southern Florida. Subsequent to the flooding, many high-water marks were identified, described, and surveyed along the south- eastern coast of Florida (Miami to Key Largo) and at selected areas along the southwestern coast of Florida (Flamingo to Goodland). Descriptions of these 336 high-water makrs are presented in tabular form in this report and their locations are plotted on nineteen 7.5-minute topographic quadrangle maps. For the southeastern coast, north-south profiles of the high-water makrs along the outher and inner barrier islands and the western shoreline of Biscayne Bay are presented. Average storm-tide elevations (relative to sea level) ranged from 4 to 6 feet in northern Biscayne Bay, were as much as 17 feet on the western shoreline near the center of the bay and ranged from 3 to 6 feet in southern Biscayne Bay and Barnes Sound. Storm-tide elevations along the southwestern coast ranged from 4 to 5 feet at Flamingo and 5 to 7 feet at Goodland in the Ten Thousand Islands area.

  13. Wind damage effects of Hurricane Andrew on mangrove communities along the southwest coast of Florida, USA

    USGS Publications Warehouse

    Doyle, T.W.; Smith, T. J.; Robblee, M.B.

    1995-01-01

    On August 24, 1992, Hurricane Andrew downed and defoliated an extensive swath of mangrove trees across the lower Florida peninsula. Permanent field sites were established to assess the extent of forest damage and to monitor the rate and process of forest recovery. Canopy trees suffered the highest mortality particularly for sites within and immediately north of the storm's eyewall. The type and extent of site damage, windthrow, branch loss, and defoliation generally decreased exponentially with increasing distance from the storm track. Forest damage was greater for sites in the storm's right quadrant than in the left quadrant tor the same given distance from the storm center. Stand exposure, both horizontally and vertically, increased the susceptibility and probability of forest damage and accounted for much of the local variability. Slight species differences were found. Laguncularia racemosa exceeded Avicennia germinans and Rhizophora mangle in damage tendency under similar wind conditions. Azimuths of downed trees were strongly correlated with maximum wind speed and vector based on a hurricane simulation of the storm. Lateral branch loss and leaf defoliation on sites without windthrow damage indicated a degree of crown thinning and light penetration equivalent to treefall gaps under normally intact forest conditions. Mangrove species and forests are susceptible to catastrophic disturbance by hurricanes; the impacts of which are significant to changes in forest structure and function.

  14. Simulation of Water Balance and Forest Treatment Effects at the H.J. Andrews Experimental Forest

    SciTech Connect

    Waichler, Scott R.; Wemple, Beverley C.; Wigmosta, Mark S.

    2005-10-30

    The watershed model DHSVM was applied to the small watersheds WS1,2,3 in H.J. Andrews Experimental Forest (HJA), Oregon and tested for skill in simulating observed forest treatment effects on streamflow. These watersheds in the rain-snow transition zone underwent road and clearcut treatments during 1959-66 and subsequent natural regeneration. DHSVM was applied with 10 m and 1 hr resolution to 1958-98, most of the period of record. Water balance for old-growth WS2 indicated that evapotranspiration and streamflow were unlikely to be the only loss terms, and groundwater recharge was included to account for about 12% of precipitation; this term was assumed zero in previous studies. After limited calibration, overall efficiency in simulating hourly streamflow exceeded 0.7, and mean annual error was less than 10%. Model skill decreased at the margins, with overprediction of low flows and underprediction of high flows. However, statistical analyses of simulated and observed peakflows yielded similar characterizations of treatment effects. Primary simulation weaknesses were snowpack accumulation, snowmelt under rain-on-snow conditions, and production of quickflow. This challenging test of DHSVM moved the model closer to a practical tool for forest management.

  15. Low-Frequency Response Following the Passage of Hurricane Andrew on the Texas-Louisiana Shelf

    NASA Astrophysics Data System (ADS)

    Pearce, S. M.; Smith, D. C.; Dimarco, S. F.

    2009-12-01

    During August 24th through 27th in 1992, Hurricane Andrew passed through the Gulf Of Mexico almost directly over several moorings on the easternmost Louisiana shelf portion of the Texas-Louisiana Shelf (LATEX) coastal ocean monitoring program. Examination of the current meter time-series showed the existence of fast moving, long shelf waves over the entire Texas-Louisiana shelf west of the storm passage for up to 12 days after direct forcing ceased. The LATEX program featured 31 moorings each with 3 current meters over the 10, 20, 50, and 200 meter isobaths in 5 cross sectional lines with additional coverage on the 200 meter isobath from the Louisiana-Mississippi River delta, to Corpus Christi, Texas. Additionally, several pressure records from LATEX and several NOAA historical coastal tide gauge data from Sabine Pass to Port Isabella, Texas were incorporated. Raw, 3-hour low pass filtered, and 40-hour low pass filtered versions of the current data were analyzed. The pressure data used were detided using a least squares fit, and the tidal records were detided using the NOAA predicted tides for that location. All data were analyzed using a wavelet analysis to determine the spectra over time. The analyzed data shows that the shelf response was largely dominated in the internal Kelvin wave mode. The wave propagated towards the west on the shelf at approximately 400 km/day. These results are contrasted and compared with wave modes predicted for coastal trapped wave solutions. The output of a coastal ocean model simulation using a forced wind field similar to the storm are also contrasted and compared with the observed data.

  16. Damage to unburied flowlines in the Gulf of Mexico during Hurricane Andrew

    SciTech Connect

    Collins, J.I.

    1995-12-31

    The US Minerals Management Service reported that 454 sub-sea pipelines were damaged during Hurricane Andrew. Previously, damage to pipelines and flowlines has been reported in a series of papers by Blumberg (1964). In the present paper a formulation of the hydrodynamic loads acting on a flowline which is lying on the seafloor in the presence of waves and currents is summarized. In general, the line dynamics can be represented by a fourth order differential equation with nonlinear forcing but a method is presented which assumes that the complete response can be broken down into distinct phases from ``early motion`` to ``taut line`` to ``yield and breaking``. The selection of appropriate force coefficients and boundary layer interactions is discussed. The initial stages of pipeline migration across the seafloor is shown to be followed by loading of the flowline once all of the ``slack`` is taken up. The loads are shown to be a function of the current orientation and the maximum tension is approximately proportional to the square of the distance between risers. Comparisons of predicted and observed damages confirm that ``effective`` boundary layer thicknesses at the seafloor and typical drag and lift coefficients which were selected based on available literature are consistent with observed flowline damage. The results of sidescan sonar mosaics of pipeline migrations and reported damage are consistent with the loads predicted by relatively simple hydrodynamic/structural models. The material presented in this paper permits the evaluation of the risk of damage to unburied flowlines using relatively simple tools. Guidelines to the assumptions of force coefficients and flowline responses are provided. 20 refs., 11 figs., 2 tabs.

  17. Multi-stage impregnation of the lithospheric mantle at the Andrew Bain FZ (SWIR)

    NASA Astrophysics Data System (ADS)

    Paganelli, E.; Brunelli, D.; Bonatti, E.; Cipriani, A.; Ligi, M.

    2008-12-01

    The Southern ridge-transform intersection of Andrew Bain Fracture Zone (ABFZ) is interpreted as a "cold spot" in the mid-ocean ridge system being characterized by a negative thermal anomaly in the oceanic upper mantle. The negative thermal anomaly is associated to the cold-edge effect due to the great age contrast of the active ridge segments. During the oceanic expedition AB06-S23, in 2006, (organized by ISMAR-CNR, Bologna, Italy, and co-financed by PRNA, Italy) with the russian R/V N. Strakhov, several samples of abyssal peridotites have been collected. Textures and modal distribution of the samples have been investigated revealing a multistage impregnation history. Deep spinel-field impregnation assemblages (sp+cpx-ol) are followed by plagioclase-field patches and mineral trails (pl+cpx-ol) and late shallow gabbroic pockets and veins. The major elements mineral chemistry reveals compositional trends of low-P/T subsolidus partial- to-complete re-equilibration undergone by the upper mantle during the upwelling beneath the ridge. These samples have experienced variable degrees of melting and reacted with percolating melts of possible different composition. In particular, samples showing the lowest degrees of melting have interacted with MORB-like melts and pyroxenitic-derived melts in the spinel and plagioclase stability fields. The presence of these two kinds of melts might prove the presence of enriched portions scattered in a normal depleted mantle beneath ocean ridges. MELTS-based runs provide constraints to variable extents of pyroxenitic-derived melt interaction with the mantle source and crystallization at variable depth of the products of such an interaction. Supported by MIUR-PRIN Cofin project 2007

  18. Fluorescence biosensing strategy based on mercury ion-mediated DNA conformational switch and nicking enzyme-assisted cycling amplification for highly sensitive detection of carbamate pesticide.

    PubMed

    Wang, Xiuzhong; Hou, Ting; Dong, Shanshan; Liu, Xiaojuan; Li, Feng

    2016-03-15

    Pesticides are of great importance in agricultural and biological fields, but pesticide residues may harm the environment and human health. A highly sensitive fluorescent biosensor for the detection of carbamate pesticide has been developed based on acetylcholinesterase (AChE)-catalyzed hydrolysis product triggered Hg(2+) release coupled with subsequent nicking enzyme-induced cleavage of a duplex DNA for cycling amplification. In this protocol, two DNA probes, an unmodified single-stranded helper DNA probe 1 (HP1) and a quencher-fluorophore probe (QFP) are ingeniously designed. HP1 can be folded into hairpin configuration through T-Hg(2+)-T base pair formation. QFP, labeled with FAM and BHQ1 at its two terminals, contains the recognition sequence and the cleavage site of the nicking enzyme. In the presence of carbamate pesticide, the activity of AChE is inhibited, and the amount of the product containing the thiol group generated by the hydrolysis reaction of acetylthiocholine chloride (ACh) decreases, resulting in the release of a low concentration of Hg(2+). The number of HP1 that can be selectively unfolded would be reduced and the subsequent nicking enzyme-assisted cleavage processes would be affected, resulting in decreased fluorescence signals. The fluorescence intensity further decreases with the increase of the pesticide concentration. Therefore, the pesticide content can be easily obtained by monitoring the fluorescence signal change, which is inversely proportional to the logarithm of the pesticide concentration. The detection limit of aldicarb, the model analyte, is 3.3 μgL(-1), which is much lower than the Chinese National Standards or those previously reported. The as-proposed method has also been applied to detect carbamate pesticide residues in fresh ginger and artificial lake water samples with satisfactory results, which demonstrates that the method has great potential for practical application in biological or food safety field.

  19. Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites.

    PubMed Central

    O'Connor, T R; Laval, J

    1989-01-01

    The 2,6-diamino-4-hydroxy-5N-formamidopyrimidine (Fapy)-DNA glycosylase of Escherichia coli, which is coded for by the fpg gene, excises purine bases with ring-opened imidazoles. In addition to the DNA glycosylase activity, we report that the Fapy-DNA glycosylase of E. coli has an associated activity, resistant to EDTA, that nicks DNA at apurinic/apyrimidinic (AP) sites. The levels of Fapy-DNA glycosylase and AP-nicking activity were parallel in crude lysates of E. coli HB101 harboring different plasmids constructed from the fpg gene. The fpg gene is different from the xth, nth, and nfo genes of E. coli, whose gene products also cleave DNA at AP sites. The Fapy-DNA glycosylase was purified to electrophoretic homogeneity. During this purification, the Fapy-DNA glycosylase copurified with an AP-nicking activity using chromatographic separations based on ion-exchange, molecular weight exclusion, and hydrophobicity. The cleavage at AP sites by the Fapy-DNA glycosylase left a 5'-phosphomonoester nucleotide at one terminus. In addition, DNA containing reduced AP sites was not nicked by the Fapy-DNA glycosylase. These data suggest that the mechanism of cleavage involved beta elimination. Therefore, this activity of the Fapy-DNA glycosylase nicking DNA at AP sites should be referred to as an AP lyase. The 3' terminus did not prime nick-translation by E. coli DNA polymerase I. However, the 3' terminus becomes a substrate for nick-translation if first allowed to react with calf intestine phosphatase or the E. coli exonuclease III. These data suggest that the repair of the Fapy lesion at least to some extent results in the formation of both 5'- and 3'-phosphomonoester nucleotides and the release of the deoxyribose. Images PMID:2664776

  20. Induction of double-strand breaks by S1 nuclease, mung bean nuclease and nuclease P1 in DNA containing abasic sites and nicks.

    PubMed Central

    Chaudhry, M A; Weinfeld, M

    1995-01-01

    Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of the enzymes acted on the substrate containing discrete abasic sites. Under conditions where little or no non-specific DNA degradation was observed, all three nucleases were able to generate double-strand breaks when the bistranded abasic sites were 1 and 3 base pairs apart. However, when the abasic sites were further apart, the enzymes again failed to cleave the DNA. These results indicate that single abasic sites do not cause sufficient denaturation of the DNA to allow incision by these single-strand specific endonucleases. The reactivity of these enzymes was also investigated on DNA substrates that were nicked by DNasel or more site-specifically by endonuclease III incision at the discrete abasic sites. The three nucleases readily induced a strand break opposite such nicks. Images PMID:7479020

  1. Label-free and ratiometric detection of nuclei acids based on graphene quantum dots utilizing cascade amplification by nicking endonuclease and catalytic G-quadruplex DNAzyme.

    PubMed

    Wang, Guang-Li; Fang, Xin; Wu, Xiu-Ming; Hu, Xue-Lian; Li, Zai-Jun

    2016-07-15

    Herein, we report a ratiometric fluorescence assay based on graphene quantum dots (GQDs) for the ultrasensitive DNA detection by coupling the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for cascade signal amplifications. With o-phenylenediamine acted as the substrate of G-quadruplex/hemin DNAzyme, whose oxidization product (that is, 2,3-diaminophenazine, DAP) quenched the fluorescence intensity of GQDs (at 460nm) obviously, accompanied with the emergence of a new emission of DAP (at 564nm). The ratiometric signal variations at the emission wavelengths of 564 and 460nm (I564/I460) were utilized for label-free, sensitive, and selective detection of target DNA. Utilizing the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for amplified cascade generation of DAP, the proposed bioassay exhibited high sensitivity toward target DNA with a detection limit of 30fM. The method also had additional advantages such as facile preparation and easy operation.

  2. The nicking homing endonuclease I-BasI is encoded by a group I intron in the DNA polymerase gene of the Bacillus thuringiensis phage Bastille

    PubMed Central

    Landthaler, Markus; Shub, David A.

    2003-01-01

    Here we describe the discovery of a group I intron in the DNA polymerase gene of Bacillus thuringiensis phage Bastille. Although the intron insertion site is identical to that of the Bacillus subtilis phages SPO1 and SP82 introns, the Bastille intron differs from them substantially in primary and secondary structure. Like the SPO1 and SP82 introns, the Bastille intron encodes a nicking DNA endonuclease of the H-N-H family, I-BasI, with a cleavage site identical to that of the SPO1-encoded enzyme I-HmuI. Unlike I-HmuI, which nicks both intron-minus and intron-plus DNA, I-BasI cleaves only intron-minus alleles, which is a characteristic of typical homing endonucleases. Interestingly, the C-terminal portions of these H-N-H phage endonucleases contain a conserved sequence motif, the intron-encoded endonuclease repeat motif (IENR1) that also has been found in endonucleases of the GIY-YIG family, and which likely comprises a small DNA-binding module with a globular ββααβ fold, suggestive of module shuffling between different homing endonuclease families. PMID:12799434

  3. Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats

    PubMed Central

    Gabsalilow, Lilia; Schierling, Benno; Friedhoff, Peter; Pingoud, Alfred; Wende, Wolfgang

    2013-01-01

    Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone NHEJ can be efficiently suppressed by ‘nickases’ that produce a single-strand break rather than a double-strand break. Highly specific nickases have been produced by engineering of homing endonucleases and more recently by modifying zinc finger nucleases (ZFNs) composed of a zinc finger array and the catalytic domain of the restriction endonuclease FokI. These ZF-nickases work as heterodimers in which one subunit has a catalytically inactive FokI domain. We present two different approaches to engineer highly specific nickases; both rely on the sequence-specific nicking activity of the DNA mismatch repair endonuclease MutH which we fused to a DNA-binding module, either a catalytically inactive variant of the homing endonuclease I-SceI or the DNA-binding domain of the TALE protein AvrBs4. The fusion proteins nick strand specifically a bipartite recognition sequence consisting of the MutH and the I-SceI or TALE recognition sequences, respectively, with a more than 1000-fold preference over a stand-alone MutH site. TALE–MutH is a programmable nickase. PMID:23408850

  4. The nicking homing endonuclease I-BasI is encoded by a group I intron in the DNA polymerase gene of the Bacillus thuringiensis phage Bastille.

    PubMed

    Landthaler, Markus; Shub, David A

    2003-06-15

    Here we describe the discovery of a group I intron in the DNA polymerase gene of Bacillus thuringiensis phage Bastille. Although the intron insertion site is identical to that of the Bacillus subtilis phages SPO1 and SP82 introns, the Bastille intron differs from them substantially in primary and secondary structure. Like the SPO1 and SP82 introns, the Bastille intron encodes a nicking DNA endonuclease of the H-N-H family, I-BasI, with a cleavage site identical to that of the SPO1-encoded enzyme I-HmuI. Unlike I-HmuI, which nicks both intron-minus and intron-plus DNA, I-BasI cleaves only intron-minus alleles, which is a characteristic of typical homing endonucleases. Interestingly, the C-terminal portions of these H-N-H phage endonucleases contain a conserved sequence motif, the intron-encoded endonuclease repeat motif (IENR1) that also has been found in endonucleases of the GIY-YIG family, and which likely comprises a small DNA-binding module with a globular betabetaalphaalphabeta fold, suggestive of module shuffling between different homing endonuclease families.

  5. Evaluation of Hydraulically Significant Discontinuities in Dockum Group Mudrocks in Andrews County, Texas

    NASA Astrophysics Data System (ADS)

    Holt, R. M.; Kuszmaul, J. S.; Cao, S.; Powers, D. W.

    2013-12-01

    Triassic mudrocks of the Dockum Group (Cooper Canyon Formation) host four, below-grade landfills at the Waste Control Specialists (WSC) site in Andrews County, Texas, including: a hazardous waste landfill and three radioactive waste landfills. At the study site, the Dockum consists of mudrocks with sparse siltstone/sandstone interbeds that developed in a semi-arid environment from an ephemeral meandering fluvial system. Sedimentary studies reveal that the mudrocks are ancient floodplain vertisols (soils with swelling clays) and siltstone/sandstone interbeds are fluvial channel deposits that were frequently subaerially exposed. Rock discontinuities, including fractures and syndepositional slickensided surfaces, were mapped during the excavation of the WCS radioactive waste landfills along vertical faces prepared by the construction contractor. Face locations were selected to insure a sampled area with nearly complete vertical coverage for each landfill. Individual discontinuities were mapped and their strike, dip, length, roughness, curvature, staining, and evidence of displacement were described. In the three radioactive waste disposal landfills, over 1750 discontinuities across 35 excavated faces were mapped and described, where each face was nominally 8 to 10 ft tall and 50 to 100 ft long. Genetic units related to paleosol development were identified. On average, the orientation of the discontinuities was horizontal, and no other significant trends were observed. Mapping within the landfill excavations shows that most discontinuities within Dockum rocks are horizontal, concave upward, slickensided surfaces that developed in the depositional environment, as repeated wetting and drying cycles led to shrinking and swelling of floodplain vertisols. Fractures that showed staining (a possible indicator of past or present hydraulic activity) are rare, vertical to near-vertical, and occur mainly in, and adjacent to, mechanically stiff siltstone and sandstone interbeds

  6. Effect of Hurricane Andrew on the Turkey Point Nuclear Generating Station from August 20--30, 1992. [Final report

    SciTech Connect

    Hebdon, F.J.

    1993-03-01

    On August 24, 1992, Hurricane Andrew, a Category 4 hurricane, struck the Turkey Point Electrical Generating Station with sustained winds of 145 mph (233 km/h). This is the report of the team that the US Nuclear Regulatory Commission (NRC) and the Institute of Nuclear Power Operations (INPO) jointly sponsored (1) to review the damage that the hurricane caused the nuclear units and the utility`s actions to prepare for the storm and recover from it, and (2) to compile lessons that might benefit other nuclear reactor facilities.

  7. A tale of two storms: Surges and sediment deposition from Hurricanes Andrew and Wilma in Florida’s southwest coast mangrove forests: Chapter 6G in Science and the storms-the USGS response to the hurricanes of 2005

    USGS Publications Warehouse

    Smith, Thomas J.; Anderson, Gordon H.; Tiling, Ginger

    2007-01-01

    Hurricanes can be very different from each other. Here we examine the impacts that two hurricanes, Andrew and Wilma, had in terms of storm surge and sediment deposition on the southwest coast of Florida. Although Wilma was the weaker storm, it had the greater impact. Wilma had the higher storm surge over a larger area and deposited more sediment than did Andrew. This effect was most likely due to the size of Wilma's eye, which was four times larger than that of Andrew.

  8. Synergistic requirement of orphan nonamer-like elements and DNA bending enhanced by HMGB1 for RAG-mediated nicking at cryptic 12-RSS but not authentic 12-RSS.

    PubMed

    Numata, Masashi; Nagata, Kyosuke

    2011-08-01

    V(D)J recombination is initiated by the specific binding of the recombination activating gene (RAG) complex to the heptamer and nonamer elements within recombination signal sequence (RSS). The break points associated with some chromosomal translocations contain cryptic RSSs, and mistargeting of RAG proteins to these less conserved elements could contribute to an aberrant V(D)J recombination. Recently, we found RAG-dependent recombination in the hotspots of TEL-AML1 t(12;21)(p13;q22) chromosomal translocation by an extrachromosomal recombination assay. Here, we describe using in vitro cleavage assays that RAG proteins directly bind to and introduce nicks into TEL and AML1 translocation regions, which contain several heptamer-like sequences. The cryptic nicking site within the TEL fragment was cleaved by RAG proteins essentially depending on a 12-RSS framework, and the nicking activity was enhanced synergistically by both HMGB1 and orphan nonamer-like (NL) sequences, which do not possess counterpart heptamers. In addition, we found that DNA bending stimulated by HMGB1 is indispensable for the HMGB1- and orphan NL element-dependent enhancement of RAG-mediated nicking at the cryptic 12-RSS. Collectively, we would propose the mechanism of HMGB1-dependent enhancement of RAG-mediated nicking at a cryptic RSS through enhanced DNA bending.

  9. Mangroves, hurricanes, and lightning strikes: Assessment of Hurricane Andrew suggests an interaction across two differing scales of disturbance

    USGS Publications Warehouse

    Smith, Thomas J.; Robblee, Michael B.; Wanless, Harold R.; Doyle, Thomas W.

    1994-01-01

    The track of Hurricane Andrew carried it across one of the most extensive mangrove for ests in the New World. Although it is well known that hurricanes affect mangrove forests, surprisingly little quantitative information exists concerning hurricane impact on forest structure, succession, species composition, and dynamics of mangrove-dependent fauna or on rates of eco-system recovery (see Craighead and Gilbert 1962, Roth 1992, Smith 1992, Smith and Duke 1987, Stoddart 1969).After Hurricane Andrew's passage across south Florida, we assessed the environmental damage to the natural resources of the Everglades and Biscayne National Parks. Quantitative data collected during subsequent field trips (October 1992 to July 1993) are also provided. We present measurements of initial tree mortality by species and size class, estimates of delayed (or continuing) tree mortality, and observations of geomorphological changes along the coast and in the forests that could influence the course of forest recovery. We discuss a potential interaction across two differing scales of disturbance within mangrove forest systems: hurricanes and lightning strikes.

  10. Mechanism of action of nalidixic acid: Purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme

    PubMed Central

    Sugino, Akio; Peebles, Craig L.; Kreuzer, Kenneth N.; Cozzarelli, Nicholas R.

    1977-01-01

    A target protein for nalidixic and oxolinic acids in Escherichia coli, the nalA gene product (Pnal), was purified to homogeneity as judged by gel electrophoresis, using an in vitro complementation assay. It is a dimer of identical 110,000-dalton subunits. A polypeptide of this molecular weight is uniquely induced by a λ nalA transducing phage, thereby showing that the purified Pnal is a product of the nalA gene. Nalidixic and oxolinic acids inhibit DNA gyrase activity and induce formation of a relaxation complex analogue. Treatment of the complex with sodium dodecyl sulfate causes a doublestrand break in the DNA substrate and the resulting linear molecule seems covalently bound to protein. Complex formation, unlike the introduction of supertwists, does not require ATP or relaxed circular DNA and is insensitive to novobiocin. DNA gyrase from a strain with a nalA mutation conferring drug resistance (nalAr) is 1/100 as sensitive to oxolinic and nalidixic acids with respect to inhibition of supertwisting and induction of the pre-linearization complex. Addition of Pnal restores drug sensitivity and stimulates DNA gyrase activity. DNA gyrase preparations and Pnal catalyze a third reaction sensitive to nalidixic and oxolinic acids, the ATP-independent relaxation of supertwister DNA. Relaxation by gyrase from nalAr cells is drug resistant. The nicking-closing activity is distinct from E. coli ω protein in several properties, including the ability to relax positively supertwisted DNA. We postulate that the nalA gene product occurs in two molecular forms, as Pnal and as a gyrase component. Both forms catalyze nicking-closing, and inhibition of this activity by nalidixic and oxolinic acids may account for the inhibition of DNA synthesis by these drugs. Images PMID:200930

  11. Plant phenology patterns at three sites on the H.J. Andrews Experimental Forest, Oregon, 1987 to 2007.

    NASA Astrophysics Data System (ADS)

    Shaw, D. C.; Creel, C.; Downing, G.; Remillard, S.; O'Connell, K.

    2007-12-01

    Plant phenology data has been collected at three sites on the H.J. Andrews Experimental Forest, Oregon since the late 1970's. The sites were visited once every three weeks year-round. Current efforts to clean and archive this data are on-going. Here we present on a 20 year data set from 1987 to 2007. The three sites are located at Watersheds (WS) 10, 8, and 7/6, at an elevation of 466 m, 993 m, and 905/950 m respectively. Forests were old growth (WS 8) and regenerating clearcuts (WS 6, 10), or shelterwood clearcut with overstory removed in 1984 (WS 7) dominated by Douglas-fir, western hemlock, true firs, and western redcedar. One tree (Douglas-fir), two evergreen shrubs, three deciduous shrubs, and four herbs were followed for vegetative and flowering phenology, including bud swell, bud bread, leaf expansion, leaf color change, leaf fall, flower bud swell, blooming, petal loss, fruit formation, and seed dispersal. Weather stations are located at each site and tied into a network of stations in this LTER site. Physical factors such as snow depth, snow coverage, freeze-thaw activity, as well as lichen condition were also noted. We are asking two key questions regarding plant phenology patterns. 1. Has the growing season lengthened in the mountainous watershed of the HJ Andrews, and is this similar for a low elevation site (WS 10) versus a mid elevation site (WS 8, 7/6)? 2. Do snow-pack dynamics influence plant phenology more so than temperature (degree days)?

  12. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  13. 78 FR 76812 - In the Matter of: Andrew Vincent O'Donnell, Inmate Number-62355-019, USP Atlanta, U.S...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE Bureau of Industry and Security In the Matter of: Andrew Vincent O'Donnell, Inmate Number--62355- 019, USP Atlanta, U.S. Penitentiary, P.O. Box 15060, Atlanta, GA 30315; Order Denying Export Privileges On August 1, 2011, in the U.S. District...

  14. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680..., small-arms firing range, Tyndall Air Force Base, Fla. (a) The danger zones—(1) Area No. 1. The waters of... Officer, Tyndall Air Force Base, Florida, and such agencies as he may designate. (Sec. 7, 40 Stat....

  15. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680..., small-arms firing range, Tyndall Air Force Base, Fla. (a) The danger zones—(1) Area No. 1. The waters of... Officer, Tyndall Air Force Base, Florida, and such agencies as he may designate. (Sec. 7, 40 Stat....

  16. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680..., small-arms firing range, Tyndall Air Force Base, Fla. (a) The danger zones—(1) Area No. 1. The waters of... Officer, Tyndall Air Force Base, Florida, and such agencies as he may designate. (Sec. 7, 40 Stat....

  17. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.... Warnings will be broadcast by the Air Force on Channel 16 (156.80 MHz) and Channel 13 (27.115 MHz)...

  18. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.... Warnings will be broadcast by the Air Force on Channel 16 (156.80 MHz) and Channel 13 (27.115 MHz)...

  19. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680..., small-arms firing range, Tyndall Air Force Base, Fla. (a) The danger zones—(1) Area No. 1. The waters of... Officer, Tyndall Air Force Base, Florida, and such agencies as he may designate. (Sec. 7, 40 Stat....

  20. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680..., small-arms firing range, Tyndall Air Force Base, Fla. (a) The danger zones—(1) Area No. 1. The waters of... Officer, Tyndall Air Force Base, Florida, and such agencies as he may designate. (Sec. 7, 40 Stat....

  1. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.... Warnings will be broadcast by the Air Force on Channel 16 (156.80 MHz) and Channel 13 (27.115 MHz)...

  2. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.... Warnings will be broadcast by the Air Force on Channel 16 (156.80 MHz) and Channel 13 (27.115 MHz)...

  3. A novel ultrasensitive ECL sensor for DNA detection based on nicking endonuclease-assisted target recycling amplification, rolling circle amplification and hemin/G-quadruplex.

    PubMed

    Luo, Fukang; Xiang, Guimin; Pu, Xiaoyun; Yu, Juanchun; Chen, Ming; Chen, Guohui

    2015-01-26

    In this study, we describe a novel universal and highly sensitive strategy for the electrochemiluminescent (ECL) detection of sequence specific DNA at the aM level based on Nt.BbvCI (a nicking endonuclease)-assisted target recycling amplification (TRA), rolling circle amplification (RCA) and hemin/G-quadruplex. The target DNAs can hybridize with self-assembled capture probes and assistant probes to form "Y" junction structures on the electrode surface, thus triggering the execution of a TRA reaction with the aid of Nt.BbvCI. Then, the RCA reaction and the addition of hemin result in the production of numerous hemin/G-quadruplex, which consume the dissolved oxygen in the detection buffer and result in a significant ECL quenching effect toward the O2/S2O8(2-) system. The proposed strategy combines the amplification ability of TRA, RCA and the inherent high sensitivity of the ECL technique, thus enabling low aM (3.8 aM) detection for sequence-specific DNA and a wide linear range from 10.0 aM to 1.0 pM. At the same time, this novel strategy shows high selectivity against single-base mismatch sequences, which makes our novel universal and highly sensitive method a powerful addition to specific DNA sequence detection.

  4. Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease

    PubMed Central

    Kostiuk, Georgij; Sasnauskas, Giedrius; Tamulaitiene, Giedre; Siksnys, Virginijus

    2011-01-01

    Unlike orthodox Type II restriction endonucleases that are homodimers and interact with the palindromic 4–8-bp DNA sequences, BcnI is a monomer which has a single active site but cuts both DNA strands within the 5′-CC↓CGG-3′/3′-GGG↓CC-5′ target site (‘↓’ designates the cleavage position). Therefore, after cutting the first strand, the BcnI monomer must re-bind to the target site in the opposite orientation; but in this case, it runs into a different central base because of the broken symmetry of the recognition site. Crystal-structure analysis shows that to accept both the C:G and G:C base pairs at the center of its target site, BcnI employs two symmetrically positioned histidines H77 and H219 that presumably change their protonation state depending on the binding mode. We show here that a single mutation of BcnI H77 or H219 residues restricts the cleavage activity of the enzyme to either the 5′-CCCGG-3′ or the 5′-CCGGG-3′ strand, thereby converting BcnI into a strand-specific nicking endonuclease. This is a novel approach for engineering of monomeric restriction enzymes into strand-specific nucleases. PMID:21227928

  5. Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform.

    PubMed

    Li, Xiang; Ding, Xuelian; Fan, Jing

    2015-12-07

    Sensitive and selective detection of ultralow concentrations of specific biomolecules is important in early clinical diagnoses and biomedical applications. Many types of aptasensors have been developed for the detection of various biomolecules, but usually suffer from false positive signals and high background signals. In this work, we have developed an amplified fluorescence aptasensor platform for ultrasensitive biomolecule detection based on enzyme-assisted target-recycling signal amplification and graphene oxide. By using a split molecular aptamer beacon and a nicking enzyme, the typical problem of false positive signals can be effectively resolved. Only in the presence of a target biomolecule, the sensor system is able to generate a positive signal, which significantly improves the selectivity of the aptasensor. Moreover, using graphene oxide as a super-quencher can effectively reduce the high background signal of a sensing platform. We select vascular endothelial growth factor (VEGF) and adenosine triphosphate (ATP) as model analytes in the current proof-of-concept experiments. It is shown that under optimized conditions, our strategy exhibits high sensitivity and selectivity for the quantification of VEGF and ATP with a low detection limit (1 pM and 4 nM, respectively). In addition, this biosensor has been successfully utilized in the analysis of real biological samples.

  6. Lessons learned from Hurricane Andrew: recommendations for care of the elderly in long-term care facilities.

    PubMed

    Silverman, M A; Weston, M; Llorente, M; Beber, C; Tam, R

    1995-06-01

    We report on the experience of a 500-bed, long-term care facility in Miami, Fla, which provides housing and nursing care units for patients--ranging from those who are independently ambulatory to those who are acutely ill and feeble--in preparing for, during, and in the immediate aftermath of Hurricane Andrew, which struck on August 24, 1992. The problems encountered included a massive influx of evacuated elderly to the facility, facility isolation, loss of electrical power, loss of running water, special dietary needs, and limited professional staffing due to personal property losses or loss of transportation. Overwhelmed county emergency medical services, limited access to hospitals and patient care, and difficulty in procuring supplies exacerbated the already complicated situation resulting from the storm. As a result of these catastrophic conditions, a number of challenges specific to the care of the elderly were identified. In conjunction with the Florida Department of Elder Affairs, we drafted a comprehensive blueprint that could serve as a disaster plan for other long-term care facilities facing a similar threat during the hurricane season.

  7. Health-hazard evaluation report HETA 89-183-2101, Andrew Jackson Junior High School, Cross Lanes, West Virginia

    SciTech Connect

    Elliott, L.J.; Baron, S.

    1991-02-01

    In response to a request submitted by teachers and the principal of the Andrew Jackson Junior High School (SIC-8211) in Cross Lanes, West Virginia, an evaluation was undertaken of possible hazardous conditions at the site. The symptoms experienced by the teachers included numbness and tingling of the extremities, upper airway irritation, eye irritation, inability to concentrate, and comfort complaints of too hot and too cold. The single story school building was constructed as an open space classroom configuration. It was heated, cooled and ventilated by nine individual roof mounted air handling units with ducted air supply. The false ceiling space served as the return air duct to the system. During 1981 to 1983 this open space was converted to approximately 30 classroom areas by erecting walls. The heating, ventilation, and air conditioning systems were modified only by adding a false ceiling return air plenum to accommodate the space conversion. The school had been treated for termite infestation by sub slab injection of chlordane (57749) and direct in room application of chlorpyrifos (2921882) (Dursban) and Diazinon (333415). Fluorescent light ballast burn outs over several years introduced Aroclor-1254 (11097691) into the building which was not effectively removed by the ventilation system. Several class rooms also registered too high for carbon-dioxide (124389) concentration, temperature and humidity levels. Recommendations were made to reduce the exposure potential to chlordane and Aroclor-1254, improve the ventilation of the classrooms, and improve the indoor air quality of the school.

  8. Once a physicist: Nick Horvath

    NASA Astrophysics Data System (ADS)

    Horvath, Nick

    2009-05-01

    How did you get into basketball? That's an easy one: I was tall. I had zero interest in sport at a young age and was pushed into basketball against my will when I was about eight. I hated it. The next year, however, I was so much taller than everyone else that in my first game, even though I had no idea what I was doing, I could simply hold the ball above my head and shoot over everyone. I fell in love with basketball after that first game. If I had played badly that day, it is likely that I would not have played again in my entire life.

  9. Environmental Assessment for the Expansion and Consolidation of the Base Exchange at Joint Base Andrews-Naval Air Facility Washington, Prince George’s County, Maryland

    DTIC Science & Technology

    2013-08-01

    fine sand, silt, and clay of marine origin estimated to be up to 80 feet in thickness. Other Coastal Plain deposits underlie the Calvert Formation...that upland deposits of non-hydric soils are predominant within the swale and suggest that the existing wetland areas were more a result of the...Contracts (Andrews AFB 2009), the Asbestos Management Program Plan (AFDW 2008), and the Lead-based Paint Management Plan (AFDW 2009b), which collectively

  10. Nomina nova in Platyhelminthes pro Macrorhynchus von Graff, 1882 (non [Gmelin, 1801]; non Dunker, 1843), and Leptocleidus Mueller, 1936 (non Andrews, 1922).

    PubMed

    Hornung, Jahn J

    2016-08-19

    Two genus-group names of flat-worms-Leptocleidus Mueller, 1936 and Macrorhynchus von Graff, 1882-are junior homonyms that are preoccupied by fossil diapsid reptile genera-Leptocleidus Andrews, 1922, and Macrorhynchus Dunker, 1843-and an extant teleost fish genus-Macrorhynchus [Gmelin, 1801] ex La Cépède, 1800. These are replaced by nomina nova (Pharyngodytes nom. nov.; Graffiellus nom. nov.). Macrorhynchus [Gmelin, 1801] is an objective senior synonym of Macrorhyncus Dumeríl, 1805 ex La Cépède, 1800 (syn. nov.), and a senior homonym of Macrorhynchus Dunker, 1843, and Macrorhynchus von Graff, 1882.

  11. I-PfoP3I: A Novel Nicking HNH Homing Endonuclease Encoded in the Group I Intron of the DNA Polymerase Gene in Phormidium foveolarum Phage Pf-WMP3

    PubMed Central

    Kong, Shuanglei; Liu, Xinyao; Fu, Liwen; Yu, Xiangchun; An, Chengcai

    2012-01-01

    Homing endonucleases encoded in a group I self-splicing intron in a protein-coding gene in cyanophage genomes have not been reported, apart from some free-standing homing edonucleases. In this study, a nicking DNA endonuclease, I-PfoP3I, encoded in a group IA2 intron in the DNA polymerase gene of a T7-like cyanophage Pf-WMP3, which infects the freshwater cyanobacterium Phormidium foveolarum is described. The Pf-WMP3 intron splices efficiently in vivo and self-splices in vitro simultaneously during transcription. I-PfoP3I belongs to the HNH family with an unconventional C-terminal HNH motif. I-PfoP3I nicks the intron-minus Pf-WMP3 DNA polymerase gene more efficiently than the Pf-WMP4 DNA polymerase gene that lacks any intervening sequence in vitro, indicating the variable capacity of I-PfoP3I. I-PfoP3I cleaves 4 nt upstream of the intron insertion site on the coding strand of EXON 1 on both intron-minus Pf-WMP3 and Pf-WMP4 DNA polymerase genes. Using an in vitro cleavage assay and scanning deletion mutants of the intronless target site, the minimal recognition site was determined to be a 14 bp region downstream of the cut site. I-PfoP3I requires Mg2+, Ca2+ or Mn2+ for nicking activity. Phylogenetic analysis suggests that the intron and homing endonuclease gene elements might be inserted in Pf-WMP3 genome individually after differentiation from Pf-WMP4. To our knowledge, this is the first report of the presence of a group I self-splicing intron encoding a functional homing endonuclease in a protein-coding gene in a cyanophage genome. PMID:22952751

  12. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    SciTech Connect

    Moniz, Ernest

    2014-02-03

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  13. Book Review: Maxwell's Demon 2: Entropy, classical and quantum information, computing. Harvey Leff and Andrew Rex (Eds.); Institute of Physics, Bristol, 2003, 500pp., US 55, ISBN 0750307595

    NASA Astrophysics Data System (ADS)

    Shenker, Orly R.

    2004-09-01

    In 1867, James Clerk Maxwell proposed a perpetuum mobile of the second kind, that is, a counter example for the Second Law of thermodynamics, which came to be known as "Maxwell's Demon." Unlike any other perpetual motion machine, this one escaped attempts by the best scientists and philosophers to show that the Second Law or its statistical mechanical counterparts are universal after all. "Maxwell's demon lives on. After more than 130 years of uncertain life and at least two pronouncements of death, this fanciful character seems more vibrant than ever." These words of Harvey Leff and Andrew Rex (1990), which open their introduction to Maxwell's Demon 2: Entropy, Classical and Quantum Information, Computing (hereafter MD2) are very true: the Demon is as challenging and as intriguing as ever, and forces us to think and rethink about the foundations of thermodynamics and of statistical mechanics.

  14. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    ScienceCinema

    Moniz, Ernest [U.S. Energy Secretary

    2016-07-12

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  15. A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland.

    PubMed

    Finnegan, Lucy; Garcia-Melgares, Manuel; Gmerek, Tomasz; Huddleston, W Ryan; Palmer, Alexander; Robertson, Andrew; Shapiro, Sarah; Unkles, Shiela E

    2011-10-01

    This study reports a novel study of marine biofilm formation comprising aerobic and anaerobic bacteria. Samples of quartz and feldspar, minerals commonly found on the earth, were suspended 5 m deep in the North Sea off the east coast of St. Andrews, Scotland for 5 weeks. The assemblage of organisms attached to these stones was cultivated under aerobic and anaerobic conditions in the laboratory. Bacteria isolated on Marine Agar 2216 were all Gram-negative and identified to genus level by sequencing the gene encoding 16S rRNA. Colwellia, Maribacter, Pseudoaltermonas and Shewanella were observed in aerobically-grown cultures while Vibrio was found to be present in both aerobic and anaerobic cultures. The obligate anaerobic bacterium Psychrilyobacter atlanticus, a recently defined genus, was identified as a close relative of isolates grown anaerobically. The results provide valuable information as to the main players that attach and form de novo biofilms on common minerals in sea water.

  16. Summary of the watershed-landscape analysis workshop: h. j. andrews experimental forest. Held in Blue River, Oregon on February 2-4, 1994. Forest Service general technical report

    SciTech Connect

    Grant, G.; McCain, C.; Cissel, J.

    1994-08-01

    The document summarizes the results of the watershed and landscape analysis workshop held at the H.J. Andrews Experimental Forest in February 1994. Five examples of watershed analyses conducted on public and private lands in the Pacific Northwest are described and critiqued, and future direction for watershed analysis on Federal lands is discussed.

  17. An Analysis and Comparison of Two Short Writings: "Inaugural Address at the University of St. Andrew's" by J.S. Mill and "The University of Utopia" by R.M. Hutchins, Based on Five Criteria.

    ERIC Educational Resources Information Center

    Poirier, Jeannine M.

    Focusing on the concept of education for work vs. education for living, the author presents a comparative analysis of two works on liberal education, each of which was originally delivered orally to university students: "The Inaugural Address at the University of St. Andrew" by John Stuart Mill and "The University of Utopia" by…

  18. Principal component and Tucker3 analyses of high performance liquid chromatography with diode-array detection fingerprints of crude extracts of Erythrina speciosa Andrews leaves.

    PubMed

    Soares, Patricia Kaori; Bruns, Roy Edward; Scarminio, Ieda Spacino

    2012-07-29

    Mixtures of ethanol, dichloromethane, hexane and acetone obtained according to a statistical design have been used to extract substances from Erythrina speciosa Andrew leaves for chromatographic fingerprinting. The plant extracts from each mixture were analyzed by HPLC-DAD providing UV-vis spectra for each chromatographic peak. These chromatograms and spectra for the design mixtures were then treated with principal component (PCA), Tucker3 and PARAFAC analyses. PCA indicated the existence of five different chromatographic fingerprints for the leave extracts depending on the solvent mixture composition. Different chromatographic peak areas were strongly correlated with the mixture proportions of acetone, dichloromethane and ethanol. Tucker3 and PARAFAC analyses were very useful for identifying simultaneous correlations between chromatographic peak areas, spectral band absorbances and solvent proportions. The acetone proportion was highly correlated with the area of the 3.69 min retention time peak and the spectral absorbances between 250 and 260 nm, consistent with the presence of natural polyphenols. The dichloromethane mixture proportion was strongly correlated with the 12.19 min chromatographic peak area and a single spectral absorbance at 201 nm. This spectral absorption is characteristic of the electronic structures of terpenes and alkaloids.

  19. Environmental Assessment for the Construction and Operation of a Battalion Headquarters for the U.S. Army Priority Air Transport at Joint Base Andrews-Naval Air Facility Washington, Prince George’s County, Maryland

    DTIC Science & Technology

    2012-12-01

    Wastewater Program, and Toxics/Asbestos Program. To learn more about each program click here. Polution Prevention Pollution Prevention The Air Force...PRIORITY AIR TRANSPORT AT JOINT BASE ANDREWS-NAVAL AIR FACILITY W ASillNGTON, MARYLAND INTRODUCTION The attached environmental assessment (EA) examines...the potential impacts on the environment from the Proposed Action to construct and operate a battalion headquarters for the U.S. Army Priority Air

  20. Analysis of Apoptosis in Ultraviolet-Induced Sea Cucumber (Stichopus japonicus) Melting Using Terminal Deoxynucleotidyl-Transferase-Mediated dUTP Nick End-Labeling Assay and Cleaved Caspase-3 Immunohistochemistry.

    PubMed

    Yang, Jing-Feng; Gao, Rong-Chun; Wu, Hai-Tao; Li, Peng-Fei; Hu, Xian-Shu; Zhou, Da-Yong; Zhu, Bei-Wei; Su, Yi-Cheng

    2015-11-04

    The sea cucumber body wall melting phenomenon occurs under certain circumstances, and the mechanism of this phenomenon remains unclear. This study investigated the apoptosis in the ultraviolet (UV)-induced sea cucumber melting phenomenon. Fresh sea cucumbers (Stichopus japonicus) were exposed to UV radiation for half an hour at an intensity of 0.056 mW/cm(2) and then held at room temperature for melting development. The samples were histologically processed into formalin-fixed paraffin-embedded tissues. The apoptosis of samples was analyzed with the terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling (TUNEL) assay and cleaved caspase-3 immunohistochemistry. The emergence of TUNEL-positive cells speeds up between 0.5 and 2 h after UV irradiation. Cleaved caspase-3 positive cells were obviously detected in sample tissues immediately after the UV irradiation. These results demonstrated that sea cucumber melting induced by UV irradiation was triggered by the activation of caspase-3 followed by DNA fragmentation in sea cucumber tissue, which was attributed to apoptosis but was not a consequence of autolysis activity.

  1. Spatial distribution and compositional variation of APS minerals related to uranium deposits in the Kiggavik-Andrew Lake structural trend, Nunavut, Canada

    NASA Astrophysics Data System (ADS)

    Riegler, Thomas; Quirt, Dave; Beaufort, Daniel

    2016-02-01

    The Kiggavik-Andrew Lake structural trend consists of four mineralized zones, partially outcropping, lying 2 km south of the erosional contact with the unmetamorphosed sandstone and basal conglomerates of the Paleoproterozoic Thelon Formation. The mineralization is controlled by a major E-W fault system associated with illite and sudoite alteration halos developed in the Archean metagraywackes of the Woodburn Lake Group. Aluminum phosphate sulfate (APS) minerals from the alunite group crystallized in association with the clay minerals in the basement alteration halo as well as in the overlying sandstones, which underwent mostly diagenesis. APS minerals are Sr- and S-rich (svanbergite end-member) in the sedimentary cover overlying the unconformity, whereas they are light rare earth elements (LREE)-rich (florencite end-member) in the altered basement rocks below the unconformity. The geochemical signature of each group of APS minerals together with the petrography indicates three distinct generations of APS minerals related to the following: (1) paleoweathering of continental surfaces prior to the basin occurrence, (2) diagenetic processes during the burial history of the lower unit of the Thelon sandstones, and (3) hydrothermal alteration processes which accompanied the uranium deposition in the basement rocks and partially overlap the sedimentary-diagenetic mineral parageneses. In addition, the association of a first generation of APS minerals with both detrital cerium oxide and aluminum oxy-hydroxide highlights the fact that a part of the detrital material of the basal Thelon Formation originated from eroded paleolaterite (allochthonous regolith). The primary rare earth element (REE)-bearing minerals (e.g., monazite, REE carbonates, and allanite) of the host rocks were characterized to identify the potential sources of REE. The REE chemical composition highlights a local re-incorporation of the REE released from the alteration processes in the APS minerals of

  2. Computer Modeling of Hydrology, Weathering, and Isotopic Fractionation in Andrews Creek, Rocky Mountain National Park, Colorado for Water Years 1992 through 2012

    NASA Astrophysics Data System (ADS)

    Webb, R. M. T.; Parkhurst, D. L.; Mast, A.; Clow, D. W.

    2014-12-01

    The U.S. Geological Survey's (USGS) Water, Energy, and Biogeochemical Model (WEBMOD) was used to simulate hydrology, weathering, and isotopic fractionation in the 1.7 square kilometer Andrews Creek alpine watershed. WEBMOD includes hydrologic modules derived from the USGS Precipitation Runoff Modeling System, the National Weather Service Hydro-17 snow model, and TOPMODEL. PHREEQC, a geochemical reaction model, is coupled with the hydrologic model to simulate the geochemical evolution of waters as they evaporate, mix, and react within the landscape. Major solute concentrations and δ18O were modeled over the period 1992-2012. Variations of chloride and inorganic nitrogen respond almost entirely to variations in atmospheric deposition and preferential elution of snowpack. Both evaporation and melting result in isotopic enrichment of heavy isotopes in the residual snowpack throughout the summer. Magnesium and potassium, derived mostly from weathering with some atmospheric inputs, vary seasonally with uptake during the growing season and release during the fall and winter. The weathering of granitic minerals—oligoclase, biotite, chlorite, pyrite, calcite, and formation of secondary minerals—kaolinite, goethite, gibbsite, and smectite-illite—were selected as primary reactions based on mole-balance modeling of basin outflows. The rates of these reactions were quantified by calibrating WEBMOD to match observed concentrations and loads. Exported annual loads of most weathering products are highly correlated with discharge, whereas silica loads are less correlated with discharge, suggesting a source that is more active during dry years and less active during wet years. Potential sources include net dissolution of kaolinite and smectite-illite or mineralization of colloids with high silica content. WEBMOD is a valuable tool for simulating water quality variations in response to climate change, acid mine drainage, acid rain, biological transformations, and other

  3. Interview with Andrew C. Kadak

    SciTech Connect

    Schabes, D.

    1996-01-01

    This article is an interview with the president and Chief Executive Officer of the Yankee Atomic Electric Company about a wide variety of aspects of the decommissioning of the Yankee Nuclear Power plant. Included are discussions of political aspects, decommissioning schedules, local impacts, technical issues of decommissioning, personnel management during decommissioning, etc.

  4. Spatial and Temporal Trends in the Carbon, Nitrogen, and Sulfur Isotopes of Stream DOM From 10 Watersheds at the HJ Andrews Experimental Forest.

    NASA Astrophysics Data System (ADS)

    Frentress, J.; Kendall, C.; Lajtha, K.; Jones, J.

    2008-12-01

    In order to better understand sources of dissolved organic matter (DOM) in streams from the small to large watershed scales, we initiated a one-year investigation of the chemical and isotopic characteristics of DOM at the HJ Andrews Experimental Forest (HJA) in Blue River, OR. DOM is a biologically significant loss from these watersheds, but its sources (forest floor, mineral soil, riparian zones, stream biota) are debated. Traditional chemical characterizations of DOM like SUVA and FI have been useful in conceptualizing and modeling streamflow sources, however, an improved method for assessing DOM quality is needed to adequately differentiate DOM from sources within the watershed. The isotopic characterization of inorganic molecules like nitrate has provided insight to the role of subsurface and surface processes governing the production and transport of critical nutrients, and yet to date, little work has been done to examine the usefulness of isotopic characterization of organically bound nutrients. We apply the isotopic characterization approach to DOM in order to better understand DOM production, transformation, and transport to streams in a range of watershed sizes. Major questions addressed in this research are: 1) Where in the watershed does stream DOM come from? 2) How do DOM sources vary temporally? 3) How do physical attributes of the watershed mediate DOM quality? A relatively new solid-phase extraction technique using C-18 resin was used to isolate DOM in water samples from 10 watersheds, ranging in size from 10 to 6200 hectares, on 3-week intervals from May 2007 to June 2008. The modified technique allowed for small (1 Liter) sample sizes and short processing times to reduce the costs of analysis. The capacity of carbon, nitrogen and sulfur isotopic characterizations of DOM, as well as traditional methods like SUVA and C:N, to predict physical watershed attributes (i.e. mean residence time, soil depth, elevation, gradient) and land use history (timber

  5. Deep versus shallow melt stagnation in an ultra-slow / ultra-cold ridge segment: the Andrew Bain southern RTI (SWIR)

    NASA Astrophysics Data System (ADS)

    Paganelli, E.; Brunelli, D.; Seyler, M.; Bonatti, E.; Cipriani, A.; Ligi, M.

    2011-12-01

    The Andrew Bain Fracture Zone (ABFZ) represents one of the largest transform faults in the ridge system spanning 750 km in length with a characteristic lens-shape structure. The southern Ridge-Transform Intersection represents the deepest sector of the whole South West Indian Ridge system. During the Italian-Russian expedition S23-AB06, the seafloor in the Southern Ridge Transform Intersection (RTI) has been sampled recovering only ultramafic material in the majority of the dredging sites. The sampled spinel and plagioclase peridotites show hybrid textures, characterized either by deep spinel-field impregnation assemblages (sp+cpx±opx±ol) or by plagioclase-field equilibrated patches and mineral trails (pl+cpx±ol) marked by both crystallization of newly formed plagioclase-field equilibrated trails and formation of plagioclase coronas around spinel. The ones collected from ridge axis show also late gabbroic pockets and veins, variably enriched in clinopyroxene. Overall textures account for important melt percolation/stagnation events occurred in the plagioclase and spinel field. Major and trace element distribution in pyroxenes and spinels from spinel-bearing peridotites overall follow a general melting trend accompanied by a progressive re-equilibration to lower P/T facies at all scales. However, only few samples can be linked to near fractional melting, while the majority of them shows REE pattern and trace element concentrations that cannot be reproduced by fractional melting process. Open-system melting (OSM) better reproduces measured REE patterns. Modeling melting in an open system scenario requires high residual porosity to be accounted for along with generally enriched melts to influx the melting parcel at depth. Melting at high residual porosity suggests a near-batch regime in which enriched melts stagnate in the spinel field. Inhibition of melt segregation during melt/rock interaction asks for a permeability barrier to develop in the region where the

  6. Physiology and Anatomy for Nurses and Healthcare Practitioners: A Homeostatic Approach - Third edition Clancy John McVicar Andrew J Physiology and Anatomy for Nurses and Healthcare Practitioners: A Homeostatic Approach - Third edition 768pp Hodder Arnold 9780340967591 0340967595 [Formula: see text].

    PubMed

    2010-02-10

    John Clancy and Andrew McVicar give a fascinating insight into the homeostatic mechanism for health. The content focuses on the body's adaptive responses in health and the maladaptive processes in ill-health. In turn, these processes are linked to the knowledge required by healthcare professionals in restoring health or enhancing the quality of life until death.

  7. A comparison of mercury burdens between St. Marks National Wildlife Refuge and St. Andrew Bay, Florida: Evaluation of fish body burdens and physiological responses in largemouth bass, spotted seatrout, striped mullet, and sunfish

    USGS Publications Warehouse

    Huge, D.H.; Rauschenberger, R.H.; Wieser, F.M.; Hemming, J.M.

    2011-01-01

    Musculature from the dorsal region of 130 largemouth bass (Micropterus salmoides), 140 sunfish (Lepomis sp.), 41 spotted seatrout (Cynoscion nebulosus) and 67 striped mullet (Mugil cephalus) were collected from five estuarine and five freshwater sites within the St. Marks National Wildlife Refuge and two estuarine and two freshwater sites from St. Andrew Bay, Florida, United States of America. Musculature was analyzed for total mercury content, sagittal otoliths were removed for age determination and physiological responses were measured. Largemouth bass and sunfish from the refuge had higher mercury concentrations in musculature than those from the bay. Male spotted seatrout, male striped mullet, male and female sunfish and female largemouth bass had mercury burdens positively correlated with length. The majority of all four species of fish from both study areas contained mercury levels below 1.5 part per million, the limit for safe consumption recommended the Florida Department of Health. In comparison, a significant percentage of largemouth bass and sunfish from several sampled sites, most notably Otter Lake and Lake Renfroe within St. Marks National Wildlife Refuge, had mercury levels consistent with the health department's guidelines of 'limited consumption' or 'no consumption guidelines.'

  8. Sir Henry Solomon Wellcome and Dr Andrew Balfour: an enterprise on the Nile and the early foundation of public health and medical research in the Sudan (1899-1935).

    PubMed

    Elhadd, T A

    2015-01-01

    In Sudan, modern medical practice and medical research began soon after the creation of Anglo-Egyptian Sudan at the turn of the 20th century. The benevolent involvement of Sir Henry Solomon Wellcome, and the ingenious feat of his protégé Sir Andrew Balfour, was crucial to the strong foundation of that establishment. Sir Henry Wellcome provided the financial sponsorship plus influential, logistical and moral support. Dr Balfour put great energy into making the enterprise one of the most amazing medical achievements in colonial medicine. Improvement in the public health of the capital Khartoum was emulated by other doctors working in this vast country. Research was not restricted to tropical medicine; it also encompassed agricultural and chemical research. This helped with the establishment of the first modern medical school in the country in 1924 and resulted in the medical service in Sudan being described as one of the best in the world. Many British doctors flocked to Sudan to make a fortune and to set a path for their career back in Britain.

  9. Erythrocytosis in a child due to Hb Andrew-Minneapolis [β144(HC1)Lys→Asn (AAG>AAT or AAC)] associated with a Spanish (δβ)(0)-thalassemia.

    PubMed

    Ropero, Paloma; González, Fernando A; Cela, Elena; Beléndez, Cristina; Pérez, Beatriz; Seri, Cristina; Fontanes, Emilia; Villegas, Ana; Díaz-Mediavilla, Joaquin

    2013-01-01

    We report a rare association of δβ-thalassemia (δβ-thal) and a hemoglobin (Hb) variant with high oxygen affinity in a Spanish newborn. The proband had no Hb A and showed microcytosis and hypochromia; the peripheral blood smear was compatible with a thalassemia trait. Molecular studies revealed that the proband had a Spanish (δβ)(0)-thal (inherited from his father) and also carried a de novo variant (Hb Andrew-Minneapolis) because from the point of hematology, his mother was quite normal. The hemoglobinopathies with high affinity for oxygen constitute an infrequent cause of secondary congenital erythrocytosis. The degree of erythrocytosis and the resulting clinical manifestations are highly variable, depending on the degree of altered oxygen affinity and the presence of thalassemic genes. Thus, when these variants are associated with β(0)- or δβ-thal, as in our case, the proportion of abnormal Hb is ∼100.0%, which may cause polycythemia, hyperviscosity, and iron deficiency. This type of association is very rare and few have been described, especially in children, as they would normally be detected in adults as the increased packed cell volume (PCV) also increases blood viscosity and causes the typical symptoms (cephalalgia, drowsiness, dizziness). The association of a high oxygen affinity Hb and a δβ-thal presents a greater degree of erythrocytosis than when this same variant is associated with a β(0)-thal, mainly because the Hb F percentage is usually greater in the δβ-thal, and Hb F normally shows a greater affinity for oxygen and a reduced P(50), although one must always take into account the degree of oxygen affinity of the Hb variant. Familial erythrocytosis and an abnormal electrophoresis finding are indicative of a high affinity Hb. However, the absence of these findings does not reject the possibility of hemoglobinopathies, and in these cases, functional and molecular studies would be justified and should be mandatory for the differential

  10. The Future of the Andrew File System

    ScienceCinema

    None

    2016-07-12

    The talk will discuss the ten operational capabilities that have made AFS unique in the distributed file system space and how these capabilities are being expanded upon to meet the needs of the 21st century. Derrick Brashear and Jeffrey Altman will present a technical road map of new features and technical innovations that are under development by the OpenAFS community and Your File System, Inc. funded by a U.S. Department of Energy Small Business Innovative Research grant. The talk will end with a comparison of AFS to its modern days competitors.

  11. Hispanos en la EPA: Andrew Almodovar

    EPA Pesticide Factsheets

    La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

  12. Wireless Andrew: Everywhere You Want To Be.

    ERIC Educational Resources Information Center

    Futhey, Tracy

    2000-01-01

    Describes the wireless local area network at Carnegie Mellon University. Highlights include classroom applications, particularly in the Business School; the use of laptop computers configured with wireless technology; handheld computers, including use for testing; and assuring appropriate uses of wireless technology. (LRW)

  13. Millikan, Robert Andrews (1868-1953)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Physicist, born in Morrison, IL, Nobel prizewinner (1923) for his determination of the charge on the electron by Millikan's oil-drop experiment, and confirmation of Einstein's quantum photoelectric theory. Coined the term `cosmic rays'....

  14. Nick Sagan Reflects on Voyager 1 and the Golden Record

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2013-10-01

    When scientists confirmed on 12 September that NASA's Voyager 1 spacecraft had entered interstellar space (Eos, 94(39), 339, doi:10.1002/2013EO390003), the probe was acknowledged as the first human-made object to travel into that realm. The probe and its twin, Voyager 2, each carry a 12-inch gold-plated copper disk, known as the Golden Record.

  15. The Portraiture of Nick: Scene Two, the Middle Years

    ERIC Educational Resources Information Center

    Bicehouse, Vaughn L.

    2012-01-01

    In 2007, the Autism Society of America (ASA) estimated that 1.5 million Americans and their families were affected by autism. As the current Autistic Spectrum Disorder (ASD) prevalence rates continue to rise (10%-17% each year) so does the awareness that ASD is no respecter of persons. ASD touches children of every racial, ethnic, and…

  16. The Portraiture of Nick: Scene One, the Early Years

    ERIC Educational Resources Information Center

    Bicehouse, Vaughn L.

    2012-01-01

    In 2007, the Autism Society of America (ASA) estimated that 1.5 million Americans and their families were affected by autism. As the current ASD prevalence rates continue to rise (10%-17% each year) so does the awareness that ASD is no respecter of persons. ASD touches children of every racial, ethnic, and socioeconomic background. Additionally,…

  17. General Christopher C. Andrews: Leading the Minnesota Forestry Revolution

    ERIC Educational Resources Information Center

    Rice, Anna M.

    2002-01-01

    In the nineteenth century, America's burgeoning population certainly did grab all the timber it could. Vast pine forests stretched from Maine to Dakota, and the lumber industry voraciously consumed them from east to west. In 1800, the Minnesota territory was sparsely sprinkled with fur traders and American Indians. By 1850, its bounteous forests…

  18. A resolution honoring the life of Andrew Wyeth.

    THOMAS, 111th Congress

    Sen. Casey, Robert P., Jr. [D-PA

    2009-01-27

    01/27/2009 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S946-947; text as passed Senate: CR S947; text of measure as introduced: CR S899-900) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  19. Installation Restoration Program. Phase 1. Records Search Andrews AFB. Maryland

    DTIC Science & Technology

    1985-06-01

    tS PNIISCATA- WAYN of growth include grasses, shrubs and mature deciduous trees including beech, oak and gum. Small stands of Virginia pine occur...Division 208 Carroll Building 8600 LaSalle Road Towson , MD 21204 301/828-1 535 RCRA Enforcement Section Vickie Province, Compliance US Environmental

  20. Nick translation - a new assay for monitoring DNA damage and repair in cultured human fibroblasts

    SciTech Connect

    Snyder, R.D.; Matheson, D.W.

    1985-01-01

    An in vitro assay has been developed to detect DNA damage and repair following chemical treatment of human diploid fibroblasts. DNA damage is measured by following the Escherichia coli DNA polymerase I-catalyzed incorporation of radiolabeled deoxycytidine triphosphate (dCTP) into the DNA of lysolecithin-permeabilized cells. DNA strand breaks with free 3' OH termini serve as template sites for incorporation, and decrease of this incorporation with time, following removal of the test chemical, indicates loss (repair) of initial damage. Inhibition of the DNA excision repair process by the addition of the repair inhibitors arabinofuranosyl cytosine (ara-C) and hydroxyurea (HU) during the incubation period gives rise to an increased number of template sites, manifesting itself in increased incorporation and indicating the induction of long-patch excision repair. Results presented demonstrate that all 14 direct-acting carcinogens tested and 8 of 14 carcinogens requiring metabolic activation give positive indication of DNA damage, repair, or both. Eleven of 14 noncarcinogens tested were scored as negative, the other 3 having previously been shown to interact with cellular DNA. This assay is shown to have predictive capability at least equal to that of UDS assays but to allow a broader spectrum of genotoxic effects to be analyzed.

  1. A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.

    PubMed Central

    Kohchi, T; Umesono, K; Ogura, Y; Komine, Y; Nakahigashi, K; Komano, T; Yamada, Y; Ozeki, H; Ohyama, K

    1988-01-01

    The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the N-terminal portion of the S12 protein is far from exons 2 and 3 for the C-terminal portion on the opposite DNA strand. S1-nuclease protection analysis and Northern hybridization with RNA isolated from the liverwort chloroplasts showed that: (i) the exons 1 and 2-3 of the rps12 gene with the neighboring genes were transcribed separately, (ii) the trans-splicing of intron 1 occurred after the processing of two primary transcripts to two pre-mRNAs, and (iii) there was no particular order for the splicing of intron 1 (trans) and intron 2 (cis) in the rps12 gene. We propose a bimolecular interaction model for trans-splicing by assuming that intermolecular base pairings between two pre-mRNAs result in the formation of the structure typical of group II introns except for disruption in the loop III region. This structure could be constructed in intron 1 of tobacco rps12 gene. Images PMID:3194192

  2. Dynamic Sheaths, in the Nick of Time or Past Their Prime?

    PubMed

    Krajcer, Zvonimir; Parekh, Dhaval

    2016-12-01

    Risk factors for vascular complications include a sheath to femoral artery ratio (SFAR) > 1.05, use of sheaths >19 Fr, peripheral artery disease, female gender, and a learning curve. High rates of technical and procedural success were achieved with low rates of major vascular and bleeding complications. However, SFAR > 1.05, gender, diabetes, renal insufficiency, calcification, tortuosity, or aneurysm were not independent risk factors in this study. Despite an SFAR > 1.05 in 23 patients, increased rates of vascular complications were not seen using the SoloPath sheath. These findings may increase the pool of transfemoral TAVI patients.

  3. Processing of Nonconjugative Resistance Plasmids by Conjugation Nicking Enzyme of Staphylococci

    SciTech Connect

    Pollet, Rebecca M.; Ingle, James D.; Hymes, Jeff P.; Eakes, Thomas C.; Eto, Karina Yui; Kwong, Stephen M.; Ramsay, Joshua P.; Firth, Neville; Redinbo, Matthew R.; Christie, P. J.

    2016-01-04

    Antimicrobial resistance inStaphylococcus aureuspresents an increasing threat to human health. This resistance is often encoded on mobile plasmids, such as pSK41; however, the mechanism of transfer of these plasmids is not well understood. In this study, we first examine key protein-DNA interactions formed by the relaxase enzyme, NES, which initiates and terminates the transfer of the multidrug resistance plasmid pSK41. Two loops on the NES protein, hairpin loops 1 and 2, form extensive contacts with the DNA hairpin formed at theoriTregion of pSK41, and here we establish that these contacts are essential for proper DNA cleavage and religation by the full 665-residue NES proteinin vitro. Second, pSK156 and pCA347 are nonconjugativeStaphylococcus aureusplasmids that contain sequences similar to theoriTregion of pSK41 but differ in the sequence predicted to form a DNA hairpin. We show that pSK41-encoded NES is able to bind, cleave, and religate theoriTsequences of these nonconjugative plasmidsin vitro. Although pSK41 could mobilize a coresident plasmid harboring its cognateoriT, it was unable to mobilize plasmids containing the pSK156 and pCA347 variantoriTmimics, suggesting that an accessory protein like that previously shown to confer specificity in the pWBG749 system may also be involved in transmission of plasmids containing a pSK41-likeoriT. These data indicate that the conjugative relaxase intransmechanism recently described for the pWBG749 family of plasmids also applies to the pSK41 family of plasmids, further heightening the potential significance of this mechanism in the horizontal transfer of staphylococcal plasmids.

    IMPORTANCEUnderstanding the mechanism of antimicrobial resistance transfer in bacteria such asStaphylococcus aureusis an important step toward potentially slowing the spread of antimicrobial-resistant infections. This work establishes protein-DNA interactions essential for the transfer of theStaphylococcus aureusmultiresistance plasmid pSK41 by its relaxase, NES. This enzyme also processed variantoriT-like sequences found on numerous plasmids previously considered nontransmissible, suggesting that in conjunction with an uncharacterized accessory protein, these plasmids may be transferred horizontally via a relaxase intransmechanism. These findings have important implications for our understanding of staphylococcal resistance plasmid evolution.

  4. Magnetic Contribution to Heat Capacity and Entropy of Nicke Ferrite (NiFe2O4)

    SciTech Connect

    S Ziemniak, L Anovitz, R Castelli

    2005-12-15

    The heat capacity of nickel ferrite was measured as a function of temperature over the range from 50 to 1200 C using a differential scanning calorimeter. A thermal anomaly was observed at 584.9 C, the expected Curie temperature, T{sub c}. The observed behavior was interpreted by recognizing the sum of three contributions: (1) lattice (vibrational), (2) a spin wave (magnetic) component and (3) a {lambda}-transition (antiferromagnetic-paramagnetic transition) at the Curie temperature. The first was modeled using vibrational frequencies derived from an experimentally-based ir absorption spectrum, while the second was modeled using a spin wave analysis that provided a T{sup 3/2} dependency in the low temperature limit, but incorporated an exchange interaction between cation spins in the octahedral and tetrahedral sites at elevated temperatures, as first suggested by Grimes [15]. The {lambda}-transition was fitted to an Inden-type model which consisted of two truncated power law series in dimensionless temperature (T/T{sub c}). Exponential equality was observed below and above T{sub c}, indicating symmetry about the Curie temperature. Application of the methodology to existing heat capacity data for other transition metal ferrites (AFe{sub 2}O{sub 4}, A = Fe, Co) revealed the same exponential equality, i.e., m = n = 5.

  5. Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding

    SciTech Connect

    Tewary, Sunil K.; Liang, Lingfei; Lin, Zihan; Lynn, Annie; Cotmore, Susan F.; Tattersall, Peter; Zhao, Haiyan; Tang, Liang

    2015-02-15

    Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45 Å resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins. - Highlights: • The structure of a parvovirus replication initiator protein has been determined; • The structure sheds light on mechanisms of ssDNA binding and cleavage; • The nickase active site is preconfigured for versatile metal ligand binding; • The binding site for the double-stranded replication origin DNA is identified; • A single domain integrates multiple functions in virus replication.

  6. 77 FR 59163 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... sprouts and saplings are common in the understory. With a lack of disturbance, the understory is comprised...). Herbicide, manual and mechanical methods would be applied to sprouts/seedlings within 1-2 years after the... sprouts and seedlings of tree species to maintain the woodland condition. Mechanical treatments...

  7. Environmental Assessment of Replacing Building 5015 at Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2003-09-01

    Federal Implementation Plan FONSI Finding of No Significant Impact FY fiscal year HAP hazardous air pollutant HSWA Hazardous and Solid Waste Amendments IICEP...Recovery Act (RCRA), which was further amended by the Hazardous and Solid Waste Amendments (HSWA), as any solid, liquid, contained gaseous, or semisolid

  8. Environmental Assessment of Consolidation Activities at Brandywine Receiver Site, Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2003-09-01

    Hazardous and Solid Waste Amendments IICEP Interagency and Intergovernmental Coordination for Environmental Planning INRMP Integrated Natural Resources... Hazardous and Solid Waste Amendments (HSWA), as any solid, liquid, contained gaseous, or semisolid waste, or any combination of wastes that poses a

  9. Additional dates of Sir Andrew Smith's Illustrations of the Zoology of South Africa.

    PubMed

    Low, Martyn E Y; Evenhuis, Neal L

    2014-05-14

    We update the collation of the dates of publication of Smith's Illustrations of the Zoology of South Africa provided by Waterhouse (1880) and Barnard (1950, 1952). In the case of nine parts, we are able to provide more accurate dates of publication (including day-dates for seven of these parts). For workers of invertebrate taxonomy, we provide an accurate date of publication for W. S. Macleay's volume on Annulosa. 

  10. A resolution honoring former Senator and Rear Admiral Jeremiah Andrew Denton, Jr.

    THOMAS, 113th Congress

    Sen. Sessions, Jeff [R-AL

    2014-03-31

    03/31/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S1881; text as passed Senate: CR S1874) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  11. Environmental Assessment for Aerial Application of Pesticide for Gypsy Moth Control, Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2008-04-01

    a cessation of feeding, and death by starvation. Btk is persistent on foliage for about 7-10 days. Btk formulations are available as flowable ...product for use in IPM programs. Mixing Instructions: Fill the spray tank with half the required amount of water. Begin agitation and add the required

  12. Environmental Assessment for the Upgrade of the Andrews Air Force Base Airfield Security System

    DTIC Science & Technology

    2004-11-01

    Russian olive ( Elaeagnus angustifolia ), beggar-ticks (Bidens polylepis), tall fescue (Festuca elatior), purple loosestrife (Lythrum salicaria...wineberry (Rubus phoenicolasius), tree-of-heaven (Ailanthus altissima), oriental bittersweet (Celastrus orbiculatus), autumn olive ( Elaeagnus umbellata

  13. Casting Net Assessment: Andrew W. Marshall and the Epistemic Community of the Cold War

    DTIC Science & Technology

    2012-06-01

    nickname of the diminutive sage for his soft voice and cryptic advice.”7 In contrast to this crude depiction, Marshall’s reputation is quietly... female worker was shot by her forlorn lover in an affair gone awry. On another occasion—when many erroneously assumed victory was inevitable in late...1944 during the heady days preceding the Battle of the Bulge—workers threw a debaucherous Christmas party that ended with a drunken female trying to

  14. Seizing Opportunities: Genie Tyburski--Ballard Spahr Andrews & Ingersoll, LLP, Philadelphia

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    Genie Tyburski did not set out to be a law librarian. When asked at Drexel's library school what kind of librarian she wanted to be, she was surprised that "a good one" was not one of the options. But six weeks into the semester, she landed a part-time cataloging job at Community Legal Services in Philadelphia; six months later she was…

  15. Detecting Outliers in Marathon Data by Means of the Andrews Plot

    NASA Astrophysics Data System (ADS)

    Stehlík, Milan; Wald, Helmut; Bielik, Viktor; Petrovič, Juraj

    2011-09-01

    For an optimal race performance, it is important, that the runner keeps steady pace during most of the time of the competition. First time runners or athletes without many competitions often experience an "blow out" after a few kilometers of the race. This could happen, because of strong emotional experiences or low control of running intensity. Competition pace of half marathon of the middle level recreational athletes is approximately 10 sec quicker than their training pace. If an athlete runs the first third of race (7 km) at a pace that is 20 sec quicker than is his capacity (trainability), he would experience an "blow out" in the last third of the race. This would be reflected by reducing the running intensity and inability to keep steady pace in the last kilometers of the race and in the final time as well. In sports science, there are many diagnostic methods ([3], [2], [6]) that are used for prediction of optimal race pace tempo and final time. Otherwise there is lacking practical evidence of diagnostics methods and its use in the field (competition, race). One of the conditions that needs to be carried out is that athletes have not only similar final times, but it is important that they keep constant pace as much as possible during whole race. For this reason it is very important to find outliers. Our experimental group consisted of 20 recreational trained athletes (mean age 32,6 years±8,9). Before the race the athletes were instructed to run on the basis of their subjective feeling and previous experience. The data (running pace of each kilometer, average and maximal heart rate of each kilometer) were collected by GPS-enabled personal trainer Forerunner 305.

  16. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) Area AP-1. The area is bounded by a line connecting the following coordinates (listed by latitude, then... Mariners and Broadcast Notice to Mariners. (3) Area AP-1. All persons, vessels, and other craft...

  17. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Area AP-1. The area is bounded by a line connecting the following coordinates (listed by latitude, then... Mariners and Broadcast Notice to Mariners. (3) Area AP-1. All persons, vessels, and other craft...

  18. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) Area AP-1. The area is bounded by a line connecting the following coordinates (listed by latitude, then... Mariners and Broadcast Notice to Mariners. (3) Area AP-1. All persons, vessels, and other craft...

  19. 76 FR 57043 - Andrew N. Finkel; Analysis of Proposed Consent Order to Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ... proposed order. This matter involves the advertising of a mobile software application (``app'') called Acne Pwner which respondent developed and sold in Google's Android Marketplace. Respondent claimed that Acne Pwner effectively treats acne. The instructions for this app directed consumers to hold the...

  20. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews

    PubMed Central

    Chen, Yonggan; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-01-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. PMID:25979899

  1. 75 FR 10457 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... restored to native hardwoods and pines and understory plants more typical of the Chattooga River and Blue... other desired non-native plants (including the federally endangered smooth coneflower, Echinacea... manual and mechanical treatment. Woodlands are forests with relatively low tree densities of...

  2. Sensibility Study of St Andrew Bay Rapid Response System for Naval Applications

    DTIC Science & Technology

    2005-06-01

    ballpoint pens , and print shops. 3. Exposure Effects Eating or drinking very large amounts of ethylene glycol can result in death, while large amounts...salinity does not correlate closely with variations of the tidal current, specifically away from the bay entrance. The salinity follows the net flows...A study based on 110 year record (1886-1996) of tropical storm entering the 330 km threat radius around Panama City with specific interest on

  3. Wind and Tiadal Effects on Chemical Spill in St Andrew Bay System

    DTIC Science & Technology

    2006-01-01

    ingredient in photographic developing solutions, hydraulic brake fluids and in inks used in stamp pads, ballpoint pens , and print shops. 59 5 / P.C...current, specifically away from the bay entrance. The salinity follows the net flows caused by wind driven currents and freshwater inflow events. At...More specifically , it is possible to see the swept area by a floating chemical, as well as the total, absorbed, dissolved and particulate

  4. Pre-Leonardian geology of Midland Farms field area, Andrews County, Texas

    SciTech Connect

    Mear, C.E.; Dufurrena, C.K.

    1984-01-01

    The Midland Farms (Ellenburger) oil field was discovered on September 16, 1952, with the completion of Anderson-Pritchard's 1 Fasken-24 well, drilled on an indicated single-fold seismic structure. The field produces from vuggy, fractured Ellenburger dolomite with up to 310 ft (94 m) of gross and net pay. The Midland Farms (Ellenburger) field is part of a larger structure which incorporates not only Midland Farms field, but Midland Farms, West (Devonian), Inez (Ellenburger), and parts of the Fasken (Penn) and Block 41 (Wolfcamp) fields. The structure is a complex, uplifted block composed of two doubly plunging, asymmetric anticlines bisected by at least one wrench-type fault and several normal faults. Penecontemporaneous leaching produced oomoldic porosity in the limestones. Ellenburger oil production was established in the Midland Farms area in September 1952, and has amounted to 61.6 million bbl oil and 28.5 bcf of gas from 91 wells to January 1983. Major Fusselman and Wolfcamp oil accumulations were discovered during development of the Ellenburger field. Fusselman oil was first produced in June 1953, and has totaled 10.1 million bbl of oil and 5 bcf of gas from 33 wells to January 1983. Wolfcamp production was established in January 1954 and totals 10.7 million bbl of oil and 1 bcf of gas from 39 wells. Total production from all zones including post-Leonard beds in the Midland Farms field area to date has been 210 million bbl of oil and 84 bcf of gas.

  5. Fascia Research Congress evidence from the 100 year perspective of Andrew Taylor Still.

    PubMed

    Findley, Thomas W; Shalwala, Mona

    2013-07-01

    More than 100 years ago A.T. Still MD founded osteopathic medicine, and specifically described fascia as a covering, with common origins of layers of the fascial system despite diverse names for individual parts. Fascia assists gliding and fluid flow and is highly innervated. Fascia is intimately involved with respiration and with nourishment of all cells of the body, including those of disease and cancer. This paper reviews information presented at the first three International Fascia Research Congresses in 2007, 2009 and 2012 from the perspective of Dr Still, that fascia is vital for organism's growth and support, and it is where disease is sown.

  6. Casting Net Assessment: Andrew W. Marshall and the Epistemic Community of the Cold War

    DTIC Science & Technology

    2015-02-01

    Halstead in Essex, England. Farmers, weavers, and cloth makers of English and Belgian descent populated the small town.10 Kitty, as she was...classes devoted to English , art, and the sciences remained stable while that of mathematics and foreign language classes declined; also, social...liability.15 Re- lationships continued to sour until May 1948, when, based on the legal counsel of wealthy San Francisco lawyer and MIT Radiation Lab

  7. A resolution electing Andrew B. Willison as the Sergeant at Arms and Doorkeeper of the Senate.

    THOMAS, 113th Congress

    Sen. Reid, Harry [D-NV

    2014-05-05

    05/05/2014 Submitted in the Senate, considered, and agreed to without amendment by Unanimous Consent. (consideration: CR S2625; text as passed Senate: CR S2649) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  8. 78 FR 78349 - Andrew Peklo III; Notice of Availability of Draft Environmental Assessment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... http://www.ferc.gov/docs-filing/esubscription.asp to be notified via email of new filings and issuances....gov/docs-filing/efiling.asp . Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You...

  9. San Andres dolomite reservoir, Emma field, Andrews County, Texas: Depositional facies and diagenesis

    SciTech Connect

    Ruppel, S.C. )

    1987-02-01

    Although nearly 100% (19 million bbl of oil) of the estimated recoverable oil in the Emma San Andres reservoir has been produced, indications are that as much as 15 million bbl of recoverable, movable oil remains. Detailed study of facies and diagenesis in the reservoir reveals considerable lateral and vertical variability, which has important implications for recovery and injection efficiencies in the field. The upper San Andres Formation in the Emma field comprises a shallowing-upward sequence of pervasively dolomitized, shallow-water subtidal to supratidal carbonates that accumulated on a gently east-sloping ramp. The lowermost part of the reservoir interval is composed of laterally extensive fusulinid packstone deposited in an open-marine ramp setting. Abruptly overlying this facies is a sequence of interbedded fusulinid/algal grainstone, formed in a migrating sand-shoal complex, and burrowed, skeletal mudstone deposited on a restricted inner ramp. The grainstone is restricted to the western and central parts of the field. These deposits are overlain by pisolitic and cryptalgal mudstones and terrigenous siliciclastics of supratidal origin. Anydrite is abundant throughout the section. Porosity and permeability are facies controlled. Highest permeabilities are developed in shoal complex grainstone (100 md) and in fusulinid packstone (925-50 md). Lateral and vertical facies variations resulted in considerable reservoir heterogeneity. Trace-element and isotope data indicate that most of the San Andres dolomite and associated sulfate (anhydrite) precipitated from downward-moving, seawater-derived brines during shallow burial in the late Guadalupian. Subsequent anhydrite dissolution has locally enhanced original porosity.

  10. Environmental Assessment, Managing Flight Obstructions to Preserve Safety at Andrews Air Force Base

    DTIC Science & Technology

    2002-12-01

    highbush blueberry ( Vaccinium corymbosa) and low growing trees such as American holly (Ilex opaca), eastern redbud (Cercis canadensis), and flowering...The shrub layer is composed mainly of acid-loving plants such as blueberries ( Vaccinium sp.), huckleberries (Gaylussacia sp.), mountain laurel (Kalmia...Cornus florida), eastern redbud (Cercis canadensis), blueberries ( Vaccinium sp.), and southern arrowwood (Viburnum dentatum). Tree removal would

  11. Andrews AFB, Washington DC Revised Uniform Summary of Surface Weather Observations (RUSSWO) Parts A-F.

    DTIC Science & Technology

    1981-10-15

    8217 . Obs. me" me. of Now $ Wilk ’rompateteh Ra.. "Io. 0 F 32 -67F _73_ &$POP 93 F TeToi P Itn ~ 0.. P .2.. _ ___.._____m ,~r, GL..BAL CLIMATOLOGY BRANCH U...m. of Homes wIui Tompem.... Rai. Nwe. 5380 69 4614.64 93 sft P 322 -F- 57p .-732P cup .-F-92P- T-1e U, Dy 161b 31408014 5586 60. 7.5 9301 93 W.. 1.1b...25 25 25 2912111/23 25 2 II 9 6 -’ 21 . . :J 1" 3 260 ( > / 19 a * - 8 18 221 Elamen (X) U r Ne. Obs. MAb Ne. of Now @ wim Tem twuo($11 v 32eNu.S0 P

  12. Enhancing Pupils' Aspirations to University: The St Andrews Sutton Trust School Experience

    ERIC Educational Resources Information Center

    Lasselle, Laurence; Keir, Fraser; Smith, Ian

    2009-01-01

    The Sutton Trust Summer School offers to S5/Year 12 pupils the opportunity to sample student life for a week at one of five selecting universities in the UK. Most of the participants on the Sutton Trust Summer School will be the first generation in their family to attend university and have come from schools which traditionally send low numbers to…

  13. Detectives Nemorin and Andrews Anti-Gun Trafficking Act of 2013

    THOMAS, 113th Congress

    Rep. King, Peter T. [R-NY-2

    2013-02-14

    04/08/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  14. Talking about Life

    NASA Astrophysics Data System (ADS)

    Impey, Chris

    2010-08-01

    Preface; Part I. Introduction Timothy Ferris, Iris Fry, Steven Dick, Ann Druyan, Pinky Nelson, Neil Tyson, Steve Benner and William Bains; Part II. Earth Roger Buick, Lynn Rothschild, John Baross, Joe Kirschvink, Andrew Knoll, Simon Conway Morris, Roger Hanlon and Lori Marino; Part III. Solar System Chris McKay, David Grinspoon, Jonathan Lunine, Carolyn Porco, Laurie Leshin, Guy Consolmagno and Peter Smith; Part IV. Exoplanets Alan Boss, Geoff Marcy, Debra Fischer, Sara Seager, David Charbonneau and Vikki Meadows; Part V. Frontiers Jill Tarter, Seth Shostak, Ray Kurzweil, Nick Bostrom, Paul Davies, Martin Rees, Ben Bova and Jennifer Michael Hecht; Reading list; Glossary; Index.

  15. Nickel(I) and nickel(III) complexes of substituted tetraaza macrocycles formed by pulse radiolysis and electrochemistry of nickel(II) precursors

    SciTech Connect

    Bernhardt, P.V.; Lawrance, G.A.; Sangster, D.F.

    1988-11-02

    The square-planar nickel(II) complexes of the ligands 8-methyl-8-nitro-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 8-amino-8-methyl-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 3,7-bis(2-aminoethyl)-1,3,5,7-tetraazabicyclo(3.3.1)nonane, and 9-methyl-9-nitro-1,4,7,11-tetraazacyclotridecane (I-IV) react rapidly with hydroxyl radicals and aquated electrons (e/sub aq/). The initial transient products of these reactions decay via first-order kinetics within a few milliseconds in neutral aqueous solution at 22/degrees/C in all cases. Electronic spectra and decay rate constants, as well as formation rate constants, are reported for all transients. Reaction of the nitro-substituted complexes with e/sub aq/ led to electron addition to the nitro group rather than to the metal center; otherwise, a Ni/sup I/ transient is observed. Following reaction with OH, the product of the initial decay remains a Ni/sup III/ species. This is more long-lived, and stabilization of Ni/sup III/ by axial coordination of the pendant amine in II is indicated. No notable stabilization of Ni/sup I/ or Ni/sup III/ from the presence of the bicyclic azamethylene football in I-III occurs. Cyclic voltammetry in acetonitrile identified both one-electron oxidation and one-electron reduction processes for the nickel(II) complexes, as well as nitro group reduction, where this group was pendant to the macrocycle. 34 references, 3 figures, 3 tables.

  16. Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification

    PubMed Central

    Wei, Hua; Zhao, Guojie; Hu, Tianyu; Tang, Suming; Jiang, Jiquan; Hu, Bo; Guan, Yifu

    2016-01-01

    We used a novel asymmetric cleavage analysis method based on rolling circle amplification (RCA) to determine the effects of LNA modification of substrate on the two subunits of R.BbvCI cleavage. We designed two sets of cleavage circular substrates by using two different ligation strategies and analyzed the single strand cleavage efficiency affected by different modification positions both from the cleaved strands and the uncleaved strands. Results showed that the effects of LNA on cleavage rates of modified strands and unmodified strands were both site-dependent. The Nb.BbvCI and Nt.BbvCI were affected by LNA modification in different way. Most of the modification positions showed strong inhibition of both of these two nickases cleavage. However, the modification in T3 position of bottom strand hardly affected both of the two nickases activities. The results suggested an intimated interaction between the two subunits of R.BbvCI, and the T3 position in bottom strand might be a less tight position which was hard to be disturbed. PMID:27582033

  17. Northern Hemisphere Wintertime Regional Trends Larissa Nazarenko and Nick Tausnev Columbia University/NASA Goddard Institute for Space Studies, New York, NY, USA

    NASA Astrophysics Data System (ADS)

    Nazarenko, L.; Tausnev, N.

    2013-12-01

    The amplified Arctic near-surface air temperature is one of the important features of climate change in recent decades. While there is attribution of the polar warming to human influence, winter-spring warming over northern areas of the Eurasia and the cooling of eastern Canada and southern Greenland during the 1980s and 1990s is argued to be largely associated with internal variability such as the positive phase of the Arctic Oscillation/North Atlantic Oscillation (AO/NAO). Although over the past 5-6 years the NAO is in more neutral state, some modeling studies suggest that the anthropogenic forcings, including the increased greenhouse gases and stratospheric ozone loss, may cause a higher frequency of the NAO positive phase. In addition to the amplified high latitude temperature increase, the global warming resulting from anthropogenic emissions of greenhouse gases may be linked with the effects of fluctuations of atmospheric circulation systems on precipitation distributions. The recent severe snow storms in the northwestern Europe, which is believed to be forced by the low NAO index conditions by the large extent, highlight the effect of natural fluctuations in atmospheric circulation systems on precipitation distribution. Using the GISS climate model with both anthropogenic (time-varying well-mixed greenhouse gases, ozone, tropospheric aerosols, stratospheric water vapor from methane oxidation, a parameterized indirect effect of aerosols on clouds, soot effect on snow and ice albedos, and land use changes) and natural forcings (changing volcanic aerosols, solar irradiance, and Earth orbital parameters), it is shown that human-induced increases in greenhouse gases in the last three decades of the 20th century, as well as anthropogenically forced scenarios for the twenty first century, have contributed to higher year-to year variability of the simulated NAO index reflected in fluctuations of atmospheric circulation systems, which in turn leads to the intensification/deficiency of precipitation in the different regions of the Northern Hemisphere. The model simulations produce a strengthened meridional pressure gradient over the North Atlantic during the last few decades of the twenties century, as well as in the projected twenty-first century warming scenarios. The NAO index exhibits a tendency towards the positive phase and enhancement of its intensity. The anomalously hot temperatures and heavy precipitation over Northern Europe, western Siberia, south-eastern America and northern Alaska are caused by positive NAO regime, while the negative NAO phase causes the extreme cold conditions over the northern Europe and increasing frequency of frozen precipitation over southern regions in Europe. Many proxy indicators in paleoclimate variability reconstructions and different studies of past, present and future high-risk events with global and regional models indicate that Europe will face major changes in extreme events, such as heat waves, heavy winter precipitation, extreme wind storms. RCP8.5 scenario with the strongest greenhouse warming presents cooling over major continental areas under the negative NAO phase that confirms enhanced variability and intensity of climate variability under extreme warming conditions.

  18. Assessing the skill of hydrology models at simulating the water cycle in the HJ Andrews LTER: Assumptions, strengths and weaknesses

    EPA Science Inventory

    Simulated impacts of climate on hydrology can vary greatly as a function of the scale of the input data, model assumptions, and model structure. Four models are commonly used to simulate streamflow in model assumptions, and model structure. Four models are commonly used to simu...

  19. Installation Development Environmental Assessment at Joint Base Andrews-Naval Air Facility Washington Prince George’s County, Maryland

    DTIC Science & Technology

    2013-04-01

    minimis thresholds Activity /source CO a NOx VOC SOx PM10 a PM2.5 De minimis threshold ( tpy ) b Exceeds de minimis thresholds? (yes/no) Construction and...unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT JBA proposes a program of targeted construction and demolition activities intended to improve its...proposed activities are  Construct a Helicopter Operations Facility.  Construct a new fitness center and demolish the West Fitness Center

  20. Final Environmental Assessment for FY07-11 BRAC Construction Requirements at Andrews Air Force Base, Maryland

    DTIC Science & Technology

    2007-09-01

    NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR( S ) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION...NAME( S ) AND ADDRESS(ES) Science Applications International Corporation (SAIC),8 421 St. John Industrial Drive, Suite 200,St. Louis,MO,63114 8...PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME( S ) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM( S ) 11. SPONSOR/MONITOR’S

  1. Comparison of four kinds of extraction techniques and kinetics of microwave-assisted extraction of vanillin from Vanilla planifolia Andrews.

    PubMed

    Dong, Zhizhe; Gu, Fenglin; Xu, Fei; Wang, Qinghuang

    2014-04-15

    Vanillin yield, microscopic structure, antioxidant activity and overall odour of vanilla extracts obtained by different treatments were investigated. MAE showed the strongest extraction power, shortest time and highest antioxidant activity. Maceration gave higher vanillin yields than UAE and PAE, similar antioxidant activity with UAE, but longer times than UAE and PAE. Overall odour intensity of different vanilla extracts obtained by UAE, PAE and MAE were similar, while higher than maceration extracts. Then, powered vanilla bean with a sample/solvent ratio of 4 g/100 mL was selected as the optimum condition for MAE. Next, compared with other three equations, two-site kinetic equation with lowest RMSD and highest R²(adj) was shown to be more suitable in describing the kinetics of vanillin extraction. By fitting the parameters C(eq), k₁, k₂, and f, a kinetics model was constructed to describe vanillin extraction in terms of irradiation power, ethanol concentration, and extraction time.

  2. Precision Landing System Mathematical Modeling Study Report for Andrews Air Force Base, Runway 19L, Camp Springs, MD.

    DTIC Science & Technology

    1994-11-01

    aircraft, and terrain. Results are provided as plots illustrating the predicted multipath levels, separation angles, and the resulting error plots from the...worst case contributors. Scenarios were modeled to determine the effects of the multipath sources in the modeled environment. These resulting errors ...were analyzed and compared to error tolerances (FAA-STD-022d) to determine if the errors are acceptable. The effects of the ADW environment were

  3. 78 FR 27126 - East Bay, St. Andrews Bay and the Gulf of Mexico at Tyndall Air Force Base, Florida; Restricted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... at Tyndall Air Force Base, Florida; Restricted Areas AGENCY: U.S. Army Corps of Engineers, DoD... (Corps) is proposing to amend its regulations by revising an existing restricted area regulation and establishing six new restricted areas along the Tyndall Air Force Base (AFB) facility shoreline. Tyndall AFB...

  4. 77 FR 15600 - Special Local Regulation; Emerald Coast Super Boat Grand Prix; Saint Andrew Bay; Panama City, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-16

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; Emerald Coast Super Boat Grand... crews, vessels, and persons on navigable waters during the Emerald Coast Super Boat Grand Prix high... the Emerald Coast Super Boat Grand Prix high speed boat races. Basis and Purpose On January 31,...

  5. Relationship of Ordovician and Silurian reservoir development to unconformities at Midland farms and Inez fields, Andrews County, Texas

    SciTech Connect

    Mear, C.E.; Becher, J.W.

    1986-03-01

    Hydrocarbons are being produced at Midland Farms and Inez fields from Ellenburger dolomites and Fusselman limestones. Reservoirs developed there during Ordovician and Silurian periods of minor folding and faulting, followed by regional uplift and subaerial exposure of the carbonates. Vuggy, cavernous, and solution-enlarged fracture porosity was developed in the Lower Ordovician Ellenburger dolomites prior to deposition of the overlying Middle Ordovician shales of the Simpson Group. Vuggy and cavernous porosity developed in the Lower Silurian Fusselman crinoid-ostracod-pellet packstones and grainstones before deposition of the overlying Silurian Wristen shales. Montoya siliceous limestones of Late Ordovician age were truncated during a period of pre-Silurian erosion, but porosity development is not indicated in Montoya rock cuttings. Only minor amounts of porosity developed in the Lower to Middle Devonian Thirty-one packstones and wackestones as a result of uplift and erosion in the Middle Devonian. Regional compression during the post-Mississippian enhanced doubly plunging anticlines now having up to 91 m (300 ft) of closure at the Ellenburger through Thirty-one formations at Midland Farms and Inez fields. Fractures may have developed in Paleozoic limestones during this period of folding, but reservoir enhancement appears to have resulted only in the Ellenburger dolomites. Representative porosity measurements of the Ellenburger and Fusselman pay zones cannot be made from wireline log calculations, due to the fractured, vuggy, and cavernous nature of the porosity.

  6. 76 FR 12094 - Andrew Peklo III; Notice of Application Tendered for Filing With the Commission and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ....net . i. FERC Contact: Steve Kartalia, (202) 502-6131 or Stephen.kartalia@ferc.gov . j. Cooperating... including constructing a new intake structure with a trashrack; and (2) construct a new fish...

  7. "Show Me Where You Study!"--An Interactive Project between German Language Students in Nottingham and St Andrews

    ERIC Educational Resources Information Center

    Hartung, Insa; Reisenleutner, Sandra

    2016-01-01

    Interactive projects among students of a Common European Framework of Reference for languages (CEFR) A1+/A2 level seem difficult to set up due to the limited language repertoire of the students. Thus, our aim was to take up the challenge and start a project with the objective of applying their language skills. We chose a collaborative approach to…

  8. Final Environmental Assessment Airfield Storm Drainage System Repair Joint Base Andrews-Naval Air Facility Washington, MD

    DTIC Science & Technology

    2015-06-01

    heating -ventilating- cooling systems , lawn maintenance, and general maintenance of streets and sidewalks. Existing noise levels (Leq and DNL) were...effort to rehabilitate JBA facilities including runway demolition and reconstruction. Please consider using solar geothermal systems to utilize...Final Environmental Assessment Airfield Storm Drainage System Repair Joint Base Andrews–Naval Air Facility Washington, Maryland Prepared for

  9. Multiple species of wild tree peonies gave rise to the ‘king of flowers’, Paeonia suffruticosa Andrews

    PubMed Central

    Zhou, Shi-Liang; Zou, Xin-Hui; Zhou, Zhi-Qin; Liu, Jing; Xu, Chao; Yu, Jing; Wang, Qiang; Zhang, Da-Ming; Wang, Xiao-Quan; Ge, Song; Sang, Tao; Pan, Kai-Yu; Hong, De-Yuan

    2014-01-01

    The origin of cultivated tree peonies, known as the ‘king of flowers' in China for more than 1000 years, has attracted considerable interest, but remained unsolved. Here, we conducted phylogenetic analyses of explicitly sampled traditional cultivars of tree peonies and all wild species from the shrubby section Moutan of the genus Paeonia based on sequences of 14 fast-evolved chloroplast regions and 25 presumably single-copy nuclear markers identified from RNA-seq data. The phylogeny of the wild species inferred from the nuclear markers was fully resolved and largely congruent with morphology and classification. The incongruence between the nuclear and chloroplast trees suggested that there had been gene flow between the wild species. The comparison of nuclear and chloroplast phylogenies including cultivars showed that the cultivated tree peonies originated from homoploid hybridization among five wild species. Since the origin, thousands of cultivated varieties have spread worldwide, whereas four parental species are currently endangered or on the verge of extinction. The documentation of extensive homoploid hybridization involved in tree peony domestication provides new insights into the mechanisms underlying the origins of garden ornamentals and the way of preserving natural genetic resources through domestication. PMID:25377453

  10. Supplemental Environmental Assessment of the Ambulatory Care Center at Joint Base Andrews-Naval Air Facility Washington, Maryland

    DTIC Science & Technology

    2011-06-01

    and MD Route 5 (Branch Avenue). The Patuxent River is approximately seven miles east of the base. The communities of Morningside, Woodyard, Clinton...Creek to the southeast. All of these streams ultimately flow into the Potomac or Patuxent Rivers (AAFB 2010). To manage on-base stormwater runoff...Patuxent River . The new ACC would reduce the area of impervious surfaces that could affect downstream water quality. Adherence to proper engineering

  11. In response to "The Knowledge of Our Knowledge": a reflection on McAndrews' view of epistemology.

    PubMed

    Winterstein, James

    2012-12-01

    This commentary considers one of the articles published in the first volume of this journal and reflects on the status of research and knowledge at that time. The chiropractic profession has witnessed advancement in the use of the scientific method in the past several decades, and scholarly journals have helped support this substantial growth.

  12. 75 FR 81637 - Endangered and Threatened Wildlife and Plants; Notice of Availability of the St. Andrew Beach...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... feral cats and hogs in beach mouse habitat. 4. In areas with known populations of beach mice (Tyndall... respective adjacent private lands), non-native predators, including free roaming cats and cat colonies, are...) Minimize impacts to dunes in beach mouse habitat due to recreational use. (b) Prohibit free-roaming...

  13. A Multi-Week Behavioral Sampling Tag for Sound Effects Studies: Design Trade-Offs and Prototype Evaluation

    DTIC Science & Technology

    2012-09-30

    and Bernie McConnell (bm8@st-andrews.ac.uk )1 1University of St. Andrews, St. Andrews, Fife KY16 8LB, UK Award Number: N000141210209 LONG...Florida Bernie McConnell, SMRU/U. St. Andrews Len Thomas, CREEM/U. St. Andrews Peter Tyack, SMRU/U. St. Andrews Michael Weise, ONR 4 Given

  14. Caring: some reflections on the impact of the culture care theory by McFarland & Andrews and a conversation with Leininger.

    PubMed

    Clarke, Pamela N; McFarland, Marilyn R; Andrews, Margaret M; Leininger, Madeleine

    2009-07-01

    This column is the first of two with a special focus on the construct of caring. In this dialogue, two Leininger scholars together address the questions related to the global impact on practice and the contribution of the model to scientific development in nursing. Then, in a special conversation, nurse theorist Madeleine Leininger offers her view of the impact of her work as well as some of her early experiences.

  15. Environmental magnetic implications of greigite (Fe3S4) formation in a 3 m.y. lake sediment record from Butte Valley, northern California Andrew

    USGS Publications Warehouse

    Roberts, P.; Reynolds, R.L.; Verosub, K.L.; Adam, D.P.

    1996-01-01

    Authigenic greigite (Fe3S4) has been identified in several horizons of lake beds in a 102-m core from Butte Valley, northern California, using mineral magnetic methods and x-ray diffraction analysis. The presence of greigite has several implications for the paleoenvironmental record from Butte Valley. First, its occurrence in 2.5 - 3.0 Ma strata confirms that greigite can persist in the geological record for long periods of time. Second, the detrital mineral magnetic record may be partially obscured by the presence of authigenic greigite and care must be taken in interpreting magnetic variations in the greigite-bearing zones as paleoclimate proxies. Third, differences in the timing of remanence acquisition for authigenic and detrital phases may compromise studies of high-frequency geomagnetic field variations. Fourth, greigite may also be significant as a paleoenvironmental indicator of lake and sediment chemistry. The magnetic detection of greigite may therefore provide important information about palcolimnological conditions. Copyright 1996 by the American Geophysical Union.

  16. Environmental Assessment for Taxiway Whiskey Supplemental Projects at Joint Base Andrews-Naval Air Facility Washington, Prince George’s County, Maryland

    DTIC Science & Technology

    2015-09-01

    pollutants and the number of exceedances in Prince George’s County in 2014. Table 3-4. Air quality standards and monitored data Pollutant Air quality...stationary sources of air emissions for each of the criteria pollutants of concern and greenhouse gases (GHGs). Monitoring and recordkeeping requirements...the Air Force and any contractors would comply with all applicable air pollution control regulations. Construction dust or emissions from construction

  17. "Catholic Higher Education: Challenges of Discipleship and Citizenship," Presentation at the Andrews McMeel University Annual Service Learning Conference. (South Bend, Indiana, November 4, 2000.)

    ERIC Educational Resources Information Center

    Vale, Carol Jean

    2000-01-01

    Explores the relationship between discipleship and citizenship, discussing ways Catholic educators can teach their students to be faithful to their Catholic ideals as they exercise the rights and responsibilities of citizenship. Service learning provides a means for integrating head and heart for students while working in a real world context.…

  18. Society News: RAS Awards 2012; Prof. Andy Fabian; Prof. John C Brown; Prof. Andrew Fazakerley; Dr. Mike Irwin; Joss Bland-Hawthorn

    NASA Astrophysics Data System (ADS)

    2012-02-01

    Each year the RAS recognizes outstanding achievement in astronomy and geophysics by the award of medals and prizes. Candidates are nominated by Fellows and the awards made by a committee of Fellows, ensuring that these scientists have earned the respect and admiration of their peers in the research community.

  19. One World, Many Cultures. Papers from the International Conference on Adult Education and the Arts (4th, St. Andrews, Scotland, July 10-14, 1995).

    ERIC Educational Resources Information Center

    Jones, David, Ed.; McConnell, Bridget, Ed.; Normie, Gerald, Ed.

    The following papers are included: "Foreword" (Bridget McConnell); "Introduction" (David J. Jones); opening addresses by George Robertson MP, Shadow Scottish Secretary, and by Charlie McConnell, Executive Director, Scottish Community Education Council; and speech by Christine Hamilton, Deputy Director, Scottish Arts Council;…

  20. Depositional and diagenetic controls on porosity permeability and oil production in McFarland/Magutex (Queen) reservoirs, Andrews County, west Texas

    SciTech Connect

    Holtz, M.H. )

    1991-03-01

    The McFarland/Magutex Queen reservoir complex lies along the northeastern edge of the Central basin platform in the west Texas Permian basin and produces oil from the Permian Queen Formation. Current production from this complex totals 42 million stock-tank barrels (MMSTB) of an estimated 219 MMSTB of original oil in place, with an estimated 90 MMSTB of remaining mobile oil (RMO). The gross pay interval contains two parasequences consisting of progradational, 30-ft-thick, upward-shoaling facies packages. Facies include shoreface, mixed tidal channel and intertidal flat, and supratidal. Elongate shoreface facies are characterized by poorly consolidated, massive to thinly laminated sandstones. The supratidal facies, which act as permeability barriers, are characterized by algal-laminated dolostone and nodular, laminated, and massive anhydrite containing halite and gypsum pseudomorphs. Highest production and the largest amount of the 90 MMSTB of RMO is associated with the shoreface and tidal-channel facies. Bulk pore volume storage capacity and permeability are also highest within these two facies. Sandstones are arkosic, containing anhydrite and dolomite cements. Accessory minerals are clays, authigenic feldspar, and dolomite. Three main pore types are recognized: interparticle, moldic and intraconstituent, and micropores. Moldic and intraconstituent porosity is associated with leached feldspars and anhydrite cement dissolution. Microporosity is associated with syndepositional, grain-coating corrensite, dissolution-enhanced feldspar cleavage planes, and authigenic multifaceted dolomite. Microporosity derived from clays and dolomite is formed preferentially in tidal-channel and intertidal flat facies.

  1. NIOSH testimony on Kuwait before the subcommittee on hospitals and health care, committee on veterans' affairs by J. S. Andrews, September 16, 1992

    SciTech Connect

    Not Available

    1992-09-16

    The testimony summarizes potential adverse health effects related to service in the Persian Gulf as presented by the Department of Health and Human Services. An estimated 9,000 workers from 43 different countries battled the burning oil wells in Kuwait from February 1991 through early November 1991 when the last was capped. Exposures and health effects in US military personnel, Kuwaiti citizens, and fire fighters were described. The hazards to the soldiers were largely dependent on the concentration of the pollutants in the air near the camps. Fortunately, the plume from the fires rose up to 10,000 and 12,000 feet, mixed with the air and then dispersed for several thousand miles downwind over a period of several weeks. The particles and gases contained in the plume were diluted as the plume travelled. Even so, some minor respiratory problems were present among the soldiers. Some of the hydrocarbons measured at low concentrations have been shown to produce cancer in laboratory animals only when present at higher levels of exposure. Based on the exposure information gathered, the author concludes that there will not likely be a detectable increase in lung cancer in Gulf War Veterans as a result of the oil well fires.

  2. Relationship between the bite size of diphosphine ligands and tetrahedral distortions of square-planar nickel(II) complexes: Stabilization of nickel(I) and palladium(I) complexes using diphosphine ligands with large bites

    SciTech Connect

    Miedaner, A.; DuBois, D.L. ); Haltiwanger, R.C. )

    1991-02-06

    Nickel and palladium complexes of the type (M(L{sub 2}){sub 2})(BF{sub 4}){sub 2} and (M(L{sub 2})(L{sub 2}{prime}))(BF{sub 4}){sub 2} (where L{sub 2} and L{sub 2}{prime} are diphosphine ligands) have been synthesized. The lowest energy electronic absorption band for the nickel complexes decreases in energy as the bite size of the diphosphine ligand increases. Similarly, the half-wave potentials for the Ni(II/I) and Pd(II/O) couples become more positive as the bite size increases. Structural studies of (Ni(dppm){sub 2})(BF{sub 4}){sub 2} (where dppm is bis(diphenylphosphino)methane) and (Ni-(dppb){sub 2})(PF{sub 6}){sub 2} (where dppb is 1,2-bis(diphenylphosphino)benzene) show that increasing the bite size of the diphosphine ligands results in larger tetrahedral distortions. The crystal structure of (Ni(Dppm){sub 2})(BF{sub 4}){sub 2}(C{sub 50}H{sub 44}B{sub 2}F{sub 8}NiP{sub 4}) and (Ni(Dppb){sub 2})(PF{sub 6}){sub 2}(C{sub 74}H{sub 64}F{sub 12}NiP{sub 6}) were measured and are reported herein. Calculations made using the extended Huckel theory indicate that the observed distortions may have an electronic as well as a steric component. The calculations also allow rationalization of the electronic absorption spectra, electrochemical data, and the stability of the Ni(I) and Pd(I) complexes (Ni(dppp){sub 2})(BF{sub 4}) (where dppp is 1,3-bis(diphenylphosphino)propane) and (Pd(dppx){sub 2})(BF{sub 4}) (where dppx is {alpha},{alpha}{prime}-bis(diphenylphosphino)-o-xylene). Complexes containing the ligand dppm have a marked tendency to become five-coordinate, as indicated by the structural determination of (Ni(dppm){sub 2}(CH{sub 3}CN))(PF{sub 6}){sub 2}. The crystal structure for the latter complex is reported.

  3. Theoretical study of mononuclear nickel(I), nickel(0), copper(i), and cobalt(I) dioxygen complexes: new insight into differences and similarities in geometry and bonding nature.

    PubMed

    Chen, Yue; Sakaki, Shigeyoshi

    2013-11-18

    Geometries, bonding nature, and electronic structures of (N^N)Ni(O2) (N^N = β-diketiminate), its cobalt(I) and copper(I) analogues, and (Ph3P)2Ni(O2) were investigated by density functional theory (DFT) and multistate restricted active space multiconfigurational second-order perturbation (MS-RASPT2) methods. Only (N^N)Ni(O2) takes a C(S) symmetry structure, because of the pseudo-Jahn-Teller effect, while all other complexes take a C(2V) structure. The symmetry lowering in (N^N)Ni(O2) is induced by the presence of the singly occupied δ(d(xy)-π(x)*) orbital. In all of these complexes, significant superoxo (O2-) character is found from the occupation numbers of natural orbitals and the O-O π* bond order, which is independent of the number of d electrons and the oxidation state of metal center. However, this is not a typical superoxo species, because the spin density is not found on the O2 moiety, even in open-shell complexes, (N^N)Ni(O2) and (N^N)Co(O2). The M-O and O-O distances are considerably different from each other, despite the similar superoxo character. The M-O distance and the interaction energy between the metal and O2 moieties are determined by the d(yz) orbital energy of the metal moiety taking the valence state. The binding energy of the O2 moiety is understood in terms of the d(yz) orbital energy in the valence state and the promotion energy of the metal moiety from the ground state to the valence state. Because of the participations of various charge transfer (CT) interactions between the metal and O2 moieties, neither the d(yz) orbital energy nor the electron population of the O2 moiety are clearly related to the O-O bond length. Here, the π bond order of the O2 moiety is proposed as a good measure for discussing the O-O bond length. Because the d electron configuration is different among these complexes, the CT interactions are different, leading to the differences in the π bond order and, hence, the O-O distance among these complexes. The reactivity of dioxygen complex is discussed with the d(yz) orbital energy.

  4. 76 FR 70423 - Procurement List Proposed Additions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    .../Location: Base Supply Center, Joint Base Andrews Naval Air Facility Washington, 1500 West Perimeter Road, Suite 2780, Joint Base Andrews, MD. NPA: Blind Industries & Services of Maryland, Baltimore,...

  5. Beginning With the End in Mind: Post-Conflict Operations and Campaign Planning

    DTIC Science & Technology

    2007-11-02

    Military Studies guest speaker program, 2004-05 academic year. 74 Andrew Steel and Andrew Marriot , “Briefing on Peace Support Operations,” (United...truth/interviews/perito.html. Steel, Andrew, Grp Cpt and Marriot , Andrew LTC. Briefing on Peace Support Operations. Ministry of Defence, United

  6. Integration of a Miniaturized Conductivity Sensor into an Animal-Borne Instrument

    DTIC Science & Technology

    2013-09-30

    an Animal-Borne Instrument Lars Boehme Sea Mammal Research Unit Scottish Oceans Institute University of St Andrews St Andrews, KY16 8LB United...NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of St Andrews, Scottish Oceans Institute,Sea Mammal Research Unit,St Andrews

  7. Supplemental Environmental Assessment for the Proposed Construction of Personally Owned Vehicle (POV) Parking for 113th Wing, District of Columbia Air National Guard Joint Base Andrews-Naval Facility, Washington, Prince George’s County, MD

    DTIC Science & Technology

    2013-05-01

    Capital Region (NCR). Current parking facilities within the license area afforded to the DC ANG cannot support the present staffing and the present parking...site adjacent to the proposed development area must be identified as a wetland buffer with adjacent wetland, based on observed soil vegetative and...facility outside of the license area which would replace some of the parking removed by the appropriate antiterrorism standards mandate. It should be noted

  8. A bill to designate the Federal building and United States courthouse located at 515 9th Street in Rapid City, South Dakota, as the "Andrew W. Bogue Federal Building and United States Courthouse".

    THOMAS, 111th Congress

    Sen. Johnson, Tim [D-SD

    2010-06-30

    06/30/2010 Read twice and referred to the Committee on Environment and Public Works. (All Actions) Notes: For further action, see H.R.5651, which became Public Law 111-298 on 12/14/2010. Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  9. A resolution condemning the murder of Army Private William Andrew "Andy" Long and the wounding of Army Private Quinton Ezeagwula, who were shot outside the Army-Navy Career Center in Little Rock, Arkansas on June 1, 2009.

    THOMAS, 111th Congress

    Sen. Lambert, Blanche M. [D-AR

    2009-06-16

    06/16/2009 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S6665-6666; text as passed Senate: CR S6666; text of measure as introduced: CR S6656) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  10. A resolution to authorize testimony and legal representation in City of St. Paul v. Irene Victoria Andrews, Bruce Jerome Berry, John Joseph Braun, David Eugene Luce, and Elizabeth Ann McKenzie.

    THOMAS, 111th Congress

    Sen. Reid, Harry [D-NV

    2010-12-09

    12/09/2010 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S8692; text as passed Senate: CR S8692; text of measure as introduced: CR S8715) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  11. Retraction: 'Number needed to treat with 4-factor prothrombin complex concentrate for urgent warfarin reversal' by Andrew Chua, Vishal Patel, Allison Perrin, Lee Stern, Jenifer Ehreth, Laurel Omert, Christopher Hood, Julie Farley, Michael McGlynn and Liping Huang.

    PubMed

    2017-04-01

    The above abstract from the THSNA 2016 Summit Abstract Proceedings, first published online in the American Journal of Hematology on 20 July 2016 in Wiley OnlineLibrary (www.onlinelibrary.wiley.com), and in Volume 91, Issue 9, p. E427, has been retracted by agreement between the authors, the journal Editor-in-Chief, Carlo Brugnara, and Wiley Periodicals, Inc. The retraction has been agreed due to concerns from the submitting authors that the abstract was inadvertently submitted prior to receiving approval from all authors and proper review of data analytics, thereby rendering it incomplete.

  12. Environmental Assessment for Replacement of Taxiway Sierra, Taxiway Whiskey, Pad 12, and Pad 13 at Joint Base Andrews-Naval Air Facility Washington, Prince George’s County, Maryland

    DTIC Science & Technology

    2013-04-01

    daily traffic ACC asphaltic cement concrete AFDW Air Force District of Washington AFI Air Force Instruction AFPD Air Force Policy Directive ANSI...Preservation Officer, Maryland Historic Trust SO2 sulfur dioxide SOx oxides of sulfur TCE trichloroethylene TMDL total maximum daily load tpy tons...PCC) pavement. It has a 25-foot-wide by 4-inch-deep asphaltic cement concrete (ACC) shoulder on the north side and on the south side where the taxiway

  13. Creating a multi-gas proxy for Delta 14C and atmospheric fossil fuel-CO2. Kevin Coakley, John Miller , Scott Lehman, Stephen Montzka, Colm Sweeney, Arlyn Andrews , Ben Miller

    NASA Astrophysics Data System (ADS)

    Coakley, K. J.; Miller, J. B.; Lehman, S.; Montzka, S. A.; Andrews, A. E.; Miller, B. R.

    2013-12-01

    The C14:C12 ratio of atmospheric CO2 (expressed as Delta 14C) is the gold standard measurement to derive the portion of observed atmospheric CO2 gradients resulting from combustion of fossil fuels (CO2-ff). This is because fossil fuels are devoid of 14C, unlike all other sources and sinks that impact atmospheric Delta14C. With enough 14C measurements, independent, 'top-down', estimation of US fossil fuel-CO2 emissions should be possible. However, our ability to make carbon-14 measurements is severely constrained by cost, accessibility to accelerator mass spectrometers (AMS) and the volume of air required to make high precision (~0.2 %) measurements of 14CO2 (mixing ratio is ~ 4e-16 mol/mol). Thus, Delta 14C is currently measured in only a small subset of NOAA/ESRL Global Monitoring Division (GMD) tall-tower and aircraft air samples. Here, we present a Projection Pursuit Regression (PPR) model to predict CO2-ff measured at different times and altitudes in terms of surrogate gases that are more widespread and relatively inexpensive to measure. This method would, in effect, allow expansion of Delta 14C measurements by factor of ~3 or 4 throughout North America. To create a proxy for CO2-ff, we take advantage of the observed correlations between (Delta 14C-derrived) CO2-ff and regional-scale enhancements of a wide array of anthropogenic gases, like CO, SF6, and halo- and hydro-carbons. We select the complexity and form of the PPR model by cross validation where validation data prediction error is minimized. In cross validation, the prediction model is based on the training data and not the validation data. We quantify prediction model performance with test data excluded from the model development process. According to cross validation, the PPR model is superior to a simpler linear model. Comparison with test CO2-ff data shows that CO2-ff can be predicted with a root mean square error of 1.1 ppm, only slightly higher than the Delta 14C-precision limit for CO2-ff of 1 ppm. Applying the PPR model to the large NOAA/ESRL/GMD database of surrogate gases, we can create a large set of synthetic CO2-ff data for the US. Finally, we will discuss the application of this large data set of lower-precision CO2-ff to top down determination of US fossil fuel CO2 emissions by presenting results from an Observation System Simulation Experiment (OSSE).

  14. Installation Restoration Program Phase 2. Confirmation/Quantification Stage 1 for Otis Air National Guard Base, Massachusetts, Air National Guard Support Center, Andrews Air Force Base, Maryland. Volume 2. Appendices

    DTIC Science & Technology

    1985-10-01

    Professional Geologist Ph.D. Doctor of Philosophy degree Podzcls zonal soil having surface layer of mats of organic material overlying gray leached...an aquifer per unit time per unit width of a satu- rated layer under a unit hydraulic gradient. unconfined an aquifer condition in which the water...cyanide, phenol, PCB, iron, copper, cadmium, chromium, lead, arsenic, nickel, and the Safe Drinking Water Act pesticides listed in Attachment 1. f

  15. The Role of Science in Managed Aquifer Recharge--the Equus Beds aquifer near Wichita, Kansas Andrew Ziegler, Director Brian Kelly, Office Chief Michael Jacobs, Manager of Water Planning and Production Debra Ary, Engineer, Water Systems Planning (Invited)

    NASA Astrophysics Data System (ADS)

    Ziegler, A. C.; Jacobs, M.; Ary, D.; Kelly, B.

    2013-12-01

    Data collection and interpretation using statistical, geochemical, and numerical simulation tools are essential parts of a long-term cooperative study between the city of Wichita, U.S. Geological Survey, and others to describe water quantity and quality conditions in a 165 square-mile part of the Equus Beds aquifer and Arkansas and Little Arkansas Rivers. The Equus Beds aquifer, eastern part of the High Plains Aquifer in south-central Kansas, is a vital water resource for agriculture and city of Wichita. Withdrawals for public supply began in the 1940s and agricultural irrigation began in the 1950-60s. These withdrawals led to water-level declines of up to 40 feet (historic low in 1993), a storage loss of 250,000 acre feet compared to predevelopment, and may enhance movement of chloride contamination from a past oilfield disposal area near Burrton and from natural chloride along the Arkansas River. Monitoring data and modeling show chloride near Burrton moved about 3 miles in 45 years, is about 1 mile away from the nearest public supply wells, and will continue to move for decades to centuries making the water unusable for irrigation or water supply without treatment. These concerns led to development of Wichita's 1993 integrated local water-supply plan that increased use of Cheney Reservoir and implemented aquifer storage and recovery (ASR) within the aquifer using high flows from the Little Arkansas River. ASR benefits include replacing depleted storage and slowing chloride movement. Decreased withdrawals, increased precipitation, and artificial recharge increased water levels and added 100,000 acre feet of storage through 2010, but drought since 2011 has increased withdrawals. A calibrated model will be used to simulate transport of chloride under several withdrawal scenarios using MODFLOW coupled with SEAWAT. Since 1995, water-quality data collection for more than 400 organic and inorganic compounds in surface water, treated source water for artificial recharge, and groundwater identified indicator bacteria, atrazine, chloride, sodium, nitrate, arsenic, iron, and manganese as constituents of concern exceeding water-quality criteria in baseline samples. Techniques were developed to estimate Little Arkansas River water quality in real-time for treatment. Geochemical modeling using PHREEQC and PHAST shows that groundwater quality is not changed if groundwater and recharge water are of similar redox potential. If different, calcite or metal hydroxides may precipitate and decrease water infiltration. A network of 38 locations with shallow and deep wells characterizes the recharge quantities and qualities for the city of Wichita to withdraw when needed from storage. Through 2013, the Demonstration project and Phase 1 and 2 facilities (capacity 40 MGD) have artificially recharged about 2 billion gallons. Total construction costs are about $300,000,000. Data-collection, interpretative geochemical and numerical simulations and water-quality transport modeling tools developed in the past 70 years are a scientific foundation to effectively and objectively manage this aquifer system.

  16. Finding of No Significant Impact and Finding of No Practicable Aleternative for the Environmental Assessment for the 21 Point Enclosed Firing Range at Joint-Base Andrews-Naval Air Facility Washington, Maryland

    DTIC Science & Technology

    2015-03-02

    weather difficulties, or bird -aircraft strikes. Bird -aircraft strike hazard (BASH) is defined as the threat of aircraft collision with birds and other...3-3  3.1.9  Bird -Aircraft Strike Hazard (BASH...AQCR Air Quality Control Region AT/FP anti-terrorism/force protection BASH Bird -Aircraft Strike Hazard bgs below ground surface BMPs Best

  17. Installation Restoration Program. Phase 2. Confirmation/Quantification. Stage 1. Problem Confirmation Study: Otis Air National Guard Base, Massachusetts, Air National Guard Support Center, Andrews Air Force Base, Maryland.

    DTIC Science & Technology

    1985-09-04

    3 OTHER AREA WELL LOGS APPENDIX E SITE SAFETY PLAN APPENDIX F CHAIN OF CUSTODY DOCUMENTATION APPENDIX G LABORATORY ANALYTICAL METHODS APPENDIX H...Sites 3-4 3-1-1 Supplementary Analytical Protocol for Phase II Sites 3-5 3-2 Analytical Methods and Required Detection Limits 3-7 3-3 Summary of 1983...provide the Air Force with general engineering , analytical, and hydrogeological services. The Phase I, Problem Identification/Records A Search at the

  18. Letter to the Editor Re: Andrew Turner, Emily R. Kearl, Kevin R. Solman Lead and other toxic metals in playground paints from South West England Science of the Total Environment 544 (2016) 460-466.

    PubMed

    Gottesfeld, Perry; Jacobs, David E

    2016-08-15

    Turner's paper emphasizes "oral bioaccessibility" instead of focusing solely on total lead content. There is no evidence that solubility testing for lead levels in paint correlates with absorption or blood lead levels in exposed children. There are many considerations in determining exposure hazards to paint that are not evaluated in assessing solubility. Although we strongly support the conclusions and recommendations of the study, we are concerned that by reporting "oral bioaccessibility" others will focus on solubility in developing regulatory standards for lead levels in paint or in conducting exposure assessments. Standards for lead in paint should continue to be based on total lead content, not "oral bioaccessibility."

  19. Environmental Assessment for the Construction and Operation of a New Shoppette/Gas Station, Class Six Store, and Name-Brand Fast Food Store at Joint Base Andrews Camp Springs, Prince George’s County, Maryland

    DTIC Science & Technology

    2010-02-01

    requirements found in 40 CFR 126.26. Joint Base Andrews’ stormwater drainage system consists of catch basins, culverts, underground storm sewer pipes ...length, approximately 26 years old, and the pipe material consists of polyethylene (USAF 1996). However, in conjunction with two heat plants being shut...down in 2005 numerous boilers and heaters were fitted with new natural gas piping during their installation. 3.4.6 Electricity The Potomac

  20. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  1. 33 CFR 80.810 - Cape San Blas, FL to Perdido Bay, FL.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Creek as an extension of the general trend of the shoreline to continue across the inlet to St. Andrews... to the shoreline across the east entrance to St. Andrews Bay. (e) A line drawn between the...

  2. Water Ingress Failure Analysis of Whistler II Unit

    DTIC Science & Technology

    2014-08-01

    Water Ingress Failure Analysis of Whistler II Unit by Andrew J Bayba ARL-TN-0623 August 2014...Failure Analysis of Whistler II Unit Andrew J Bayba Sensors and Electron Devices Directorate, ARL...Ingress Failure Analysis of Whistler II Unit 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Andrew J Bayba

  3. C-21 Fleet: Base Optimization

    DTIC Science & Technology

    assigned to the operational support airlift mission, located at Andrews Air Force Base, Maryland and Scott Air Force Base, Illinois. The missions flown... Scott and Andrews AFB is the optimal assignment. If nine total assets were optimized, five would be assigned to Scott AFB and four to Andrews AFB

  4. Strength and Fatigue of NT551 Silicon Nitride and NT551 Diesel Exhaust Valves

    SciTech Connect

    Andrews, M.J.; Wereszczak, A.A.; Kirkland, T.P.; Breder, K.

    2000-02-01

    The content of this report is excerpted from Mark Andrew's Ph.D. Thesis (Andrews, 1999), which was funded by a DOEYOTT High Temperature Materials Laboratory Graduate Fellowship. It involves the characterization of NT551 and valves fabricated with it. Greater detail of the described issues may be found in that reference or through communications with Andrew Wereszczak.

  5. Alternative nucleophilic substrates for the endonuclease activities of human immunodeficiency virus type 1 integrase

    SciTech Connect

    Ealy, Julie B.; Sudol, Malgorzata; Krzeminski, Jacek; Amin, Shantu; Katzman, Michael

    2012-11-10

    Retroviral integrase can use water or some small alcohols as the attacking nucleophile to nick DNA. To characterize the range of compounds that human immunodeficiency virus type 1 integrase can accommodate for its endonuclease activities, we tested 45 potential electron donors (having varied size and number or spacing of nucleophilic groups) as substrates during site-specific nicking at viral DNA ends and during nonspecific nicking reactions. We found that integrase used 22 of the 45 compounds to nick DNA, but not all active compounds were used for both activities. In particular, 13 compounds were used for site-specific and nonspecific nicking, 5 only for site-specific nicking, and 4 only for nonspecific nicking; 23 other compounds were not used for either activity. Thus, integrase can accommodate a large number of nucleophilic substrates but has selective requirements for its different activities, underscoring its dynamic properties and providing new information for modeling and understanding integrase.

  6. The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase.

    PubMed

    Bauer, Robert J; Evans, Thomas C; Lohman, Gregory J S

    2016-01-01

    DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observed a significant substrate inhibition effect, as well as the inhibition of both the self-adenylylation and nick-sealing steps of T4 DNA ligase by non-nicked, non-substrate dsDNA. Inhibition by non-substrate DNA was dependent only on the total DNA concentration rather than the structure; with 1 μg/mL of 40-mers, 75-mers, or circular plasmid DNA all inhibiting ligation equally. A >15-fold reduction in T4 DNA ligase self-adenylylation rate when in the presence of high non-nicked dsDNA concentrations was observed. Finally, EMSAs were utilized to demonstrate that non-substrate dsDNA can compete with nicked dsDNA substrates for enzyme binding. Based upon these data, we hypothesize the inhibition of T4 DNA ligase by non-nicked dsDNA is direct evidence for a two-step nick-binding mechanism, with an initial, nick-independent, transient dsDNA-binding event preceding a transition to a stable binding complex in the presence of a nick site.

  7. The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase

    PubMed Central

    Bauer, Robert J.; Evans, Thomas C.; Lohman, Gregory J. S.

    2016-01-01

    DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observed a significant substrate inhibition effect, as well as the inhibition of both the self-adenylylation and nick-sealing steps of T4 DNA ligase by non-nicked, non-substrate dsDNA. Inhibition by non-substrate DNA was dependent only on the total DNA concentration rather than the structure; with 1 μg/mL of 40-mers, 75-mers, or circular plasmid DNA all inhibiting ligation equally. A >15-fold reduction in T4 DNA ligase self-adenylylation rate when in the presence of high non-nicked dsDNA concentrations was observed. Finally, EMSAs were utilized to demonstrate that non-substrate dsDNA can compete with nicked dsDNA substrates for enzyme binding. Based upon these data, we hypothesize the inhibition of T4 DNA ligase by non-nicked dsDNA is direct evidence for a two-step nick-binding mechanism, with an initial, nick-independent, transient dsDNA-binding event preceding a transition to a stable binding complex in the presence of a nick site. PMID:26954034

  8. Stress-induced cleavage of Myc promotes cancer cell survival

    PubMed Central

    Conacci-Sorrell, Maralice; Ngouenet, Celine; Anderson, Sarah; Brabletz, Thomas; Eisenman, Robert N.

    2014-01-01

    Evasion of apoptosis is critical in Myc-induced tumor progression. Here we report that cancer cells evade death under stress by activating calpain-mediated proteolysis of Myc. This generates Myc-nick, a cytoplasmic, transcriptionally inactive cleavage product of Myc. We found conversion of Myc into Myc-nick in cell lines and tissues derived from multiple cancers. In colon cancer, the production of Myc-nick is enhanced under stress conditions such as hypoxia and nutrient deprivation. Under these conditions, ectopic expression of Myc-nick promotes anchorage-independent growth and cell survival at least in part by promoting autophagy. Myc-nick also delays colon cancer cell death after treatment with chemotherapeutic drugs such as etoposide, cisplatin, and imatinib. Furthermore, colon cancer cells expressing a cleavage-resistant form of Myc undergo extensive apoptosis but are rescued by overexpression of Myc-nick. We also found that ectopic expression of Myc-nick results in the induction of the actin-bundling protein fascin, formation of filopodia, and increased cell motility—all mediators of tumor metastasis. Myc-nick-induced survival, autophagy, and motility require Myc box II (MBII), a region of Myc-nick that recruits acetyltransferases that in turn modify cytoplasmic proteins, including α-tubulin and ATG3. Our results suggest that Myc-nick-induced survival and motility contribute to colon cancer progression and metastasis. PMID:24696454

  9. Therapy of Prostate Cancer Using a Human Antibody Targeting the Type 1 Insulin-Like Growth Factor Receptor (IGF-IR)

    DTIC Science & Technology

    2009-09-01

    mediated nick end labeling assay and propidium iodide staining using the ApoDirect kit (BD Biosciences, San Jose, CA) as previously described (4...after treatment cessation using terminal deoxynucleotidyl transferase–mediated nick end labeling assay and propidium iodide staining. In animals that had...transferase ^ mediated nick end labeling assay and propidium iodide staining as described in Materials and Methods. Apoptosis is shownby FITC

  10. Environmental Impact Analysis Process. Final Environmental Impact Statement. Proposed Alaskan Radar System Over-the-Horizon Backscatter Radar Program

    DTIC Science & Technology

    1987-01-30

    Nick Anderson out in District 18, North Pole area. Sorry I wasn’t able to get more information in the local media about the Backscatter system. I was...speak. Just for my planning purposes. One, two, three, alright, thank you. Sir? Mr. Anderson: Nick Anderson again out at District 18. From the...Transportation Anchorage, AK 99503 Safety Office 701 C Street Nick Lincoln Anchorage, AK 99501 Copper Center Village Council P.O. Box 154 G. R. Moberg

  11. Blending Different Latency Traffic with Alpha-Mixing

    DTIC Science & Technology

    2006-06-01

    5. George Danezis, Roger Dingledine, and Nick Mathewson. Mixminion: Design of a type III anonymous remailer protocol. In 2003 IEEE Symposium on...Dingledine and Nick Mathewson. Anonymity loves company: Usability and the network effect. In Proceedings of Workshop on Economics and Information Security...WEIS06), June 2006. 10. Roger Dingledine, Nick Mathewson, and Paul Syverson. Tor: The Second- Generation Onion Router. In Proceedings of the 13th

  12. IMPACT OF CRITICAL ANION SOIL SOLUTION CONCENTRATION ON ALUMINUM ACTIVITY IN ALPINE TUNDRA SOIL Andrew Evans, Jr.1 , Michael B. Jacobs2, and Jason R. Janke1, (1) Metropolitan State University of Denver, Dept. of Earth and Atmospheric Sciences, (2) Dept. of Chemistry, Denver, CO, United States.

    NASA Astrophysics Data System (ADS)

    Evans, A.

    2015-12-01

    Soil solution anionic composition can impact both plant and microbial activity in alpine tundra soils by altering biochemical cycling within the soil, either through base cation leaching, or shifts in aluminum controlling solid phases. Although anions play a critical role in the aqueous speciation of metals, relatively few high altitude field studies have examined their impact on aluminum controlling solid phases and aluminum speciation in soil water. For this study, thirty sampling sites were selected on Trail Ridge Road in Rocky Mountain National Park, Estes Park, CO, and sampled during July, the middle of the growing season. Sampling elevations ranged from approximately 3560 - 3710 m. Soil samples were collected to a depth of 15.24 cm, and the anions were extracted using a 2:1 D.I. water to soil ratio. Filtered extracts were analyzed using IC and ICP-MS. Soil solution NO3- concentrations were significantly higher for sampling locations east of Iceberg Pass (EIBP) (mean = 86.94 ± 119.8 mg/L) compared to locations west of Iceberg Pass (WIBP) (mean 1.481 ± 2.444 mg/L). Both F- and PO43- soil solution concentrations, 0.533 and 0.440 mg/L, respectively, were substantially lower, for sampling sites located EIBP, while locations WIBP averaged 0.773 and 0.829 mg/L respectively, for F- and PO43-. Sulfate concentration averaged 3.869 ± 3.059 mg/L for locations EIBP, and 3.891 ± 3.1970 for locations WIBP. Geochemical modeling of Al3+ in the soil solution indicated that a suite of aluminum hydroxyl sulfate minerals controlled Al3+ activity in the alpine tundra soil, with shifts between controlling solid phases occurring in the presence of elevated F- concentrations.

  13. United States History Simulations: 1787-1868: Constitution Convention, Missouri Compromise, Treaty of Guadalupe Hidalgo, The Compromise of 1850, The Kansas/Nebraska Act, Southern Secession from the Union, and the Impeachment and Trial of Andrew Johnson. ETC Simulations Number Two.

    ERIC Educational Resources Information Center

    Hostrop, Richard W.

    This book presents simulation activities for significant events in U.S. history from 1787-1868. Intended for student involvement, the simulations require student research and practice in order to carry out the designated roles. The simulation and role play serve to involve the students actively in their learning, using both the affective and…

  14. A concurrent resolution expressing the sense of Congress that the United States Postal Service should issue a commemorative postage stamp honoring civil rights workers Andrew Goodman, James Chaney, and Michael Schwerner, and the "Freedom Summer" of 1964, and that the Citizens' Stamp Advisory Committee should recommend to the Postmaster General that such a stamp be issued.

    THOMAS, 111th Congress

    Sen. Schumer, Charles E. [D-NY

    2010-07-12

    07/15/2010 Committee on Homeland Security and Governmental Affairs referred to Subcommittee on Federal Financial Management, Government Information, Federal Services, and International Security. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  15. Comment on "Nanosecond laser textured superhydrophobic metallic surfaces and their chemical sensing applications" by Duong V. Ta, Andrew Dunn, Thomas J. Wasley, Robert W. Kay, Jonathan Stringer, Patrick J. Smith, Colm Connaughton, Jonathan D. Shephard (Appl. Surf. Sci. 357 (2015) 248-254)

    NASA Astrophysics Data System (ADS)

    Boinovich, L. B.; Emelyanenko, A. M.; Emelyanenko, K. A.; Domantovsky, A. G.; Shiryaev, A. A.

    2016-08-01

    Nowadays the problem of design of durable ecologically friendly superhydrophobic surfaces is of great importance for science and technology. A recent paper in Applied Surface Science reports the method of fabricating the superhydrophobic metallic surfaces by infrared nanosecond laser surface texturing without using hydrophobic agents. Since this method of surface texturing can be considered as one of the most suitable for various industrial applications, the nature of superhydrophobic state of surfaces produced by laser texturing in the abovementioned paper deserves to be analyzed in detail. Authors of the commented paper attributed the change in wettability to the partial deoxidation of CuO into Cu2O on the surface during storage in atmosphere. However, such interpretation of the results contradicts to the basic notions in the theory of wetting and to more accurate and detailed data. In our Comment we discuss these contradictions point by point.

  16. Targeted Silver Nanoparticles for Dual-Energy Breast X-Ray Imaging

    DTIC Science & Technology

    2013-03-01

    FOR TEMPORAL SUBTRACTION AND DUAL-ENERGY X-RAY IMAGING, Roshan Karunamuni, Ajlan Al Zaki, Anatoliy V. Popov, E. James Delikatny, Sara Gavenonis, Andrew...imaging Roshan Karunamuni1, Ajlan Al Zaki2, Anatoliy V. Popov1, E. James Delikatny1, Sara Gavenonis1, Andrew Tsourkas2, Andrew D. A. Maidment1...Carton AK, Ullberg C, Lindman K, Acciavati R, Francke T, Maidmenet ADA. Optimization of a dual- energy contrast-enhanced technique for a photon

  17. Constitutional Reform for Conflict Management

    DTIC Science & Technology

    2014-04-01

    Regulation,” in Constitutional Design for Divided Societies: Integration or Accommodation? ed. Sujit Choudhry (New York: Oxford University Press , 2008...Conflict Management, and Democracy, ed. Andrew Reynolds (New York: Oxford University Press , 2002): 37-54. Andrew Reynolds and Timothy D. Sisk...ed. Andrew Reynolds (New York: Oxford University Press , 2002): 15-36. Benjamin Reilly, Democracy in Divided Societies: Electoral Engineering for

  18. Multiscaled Mathematics in Defense Research and its Spin-offs

    DTIC Science & Technology

    2011-05-06

    Sarah Waters, James Oliver, Douglas Cochran, and many others. Air Vehicles led by Malcolm Arthur, with Sergei Timoshin, Andrew Lacey, Stephen Wilson...Luheshi, Roman Novokshanov, Andrew Belyavin, Malcolm Cook, and others. Aspects of impacts led by Richard Purvis, Guo-Xiong Wu, James Oliver, with...Allwright, Robert Bowles, Douglas Cochran, Alistair Fitt, Stephen Glavin, Anthony Hutton , George Klokkaris, Andrew Lacey, Alex Smith, Sarah Waters

  19. Exploratory Visual Analytics of a Dynamically Built Network of Nodes in a WebGL-Enabled Browser

    DTIC Science & Technology

    2014-01-01

    Exploratory Visual Analytics of a Dynamically Built Network of Nodes in a WebGL -Enabled Browser by Andrew M. Neiderer ARL-MR-860 January...January 2014 Exploratory Visual Analytics of a Dynamically Built Network of Nodes in a WebGL -Enabled Browser Andrew M. Neiderer...of Nodes in a WebGL -Enabled Browser 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Andrew M. Neiderer 5d

  20. Operations Events Census Report: Volume IV. 1981 through 1985. Sanitized Version.

    DTIC Science & Technology

    1991-04-01

    ZEITZ, JOSEPH J. 0682 ZEMANEK, ROBERT B., JR. 0935 ZERGA, DONALD L. 0918 ZIKA , ANDREW P., III 0837 ZIMMERMAN, DOUGLAS E. 0918 ZIMMERSCHIED, ALAN B...0599 Personnel Dose Information - 1984 SOCIAL GAMMA BETA SECURITY ORG DOSE DOSE NAM & CD MREM ZIKA , ANDREW P., III 0837 ZIMMERMAN, DOUGLAS E. 0918...ROBERT A. 0682 ZBLEWSKI, FRANCIS J. 0954 ZEEMIN, ROBERT R. 0975 ZEMANEK, ROBERT S. 0935 ZIKA , ANDREW P., III 0837 ZIMMERMAN, ADAM P. 0599 ZIMMERMAN

  1. Integration of a Miniaturized Conductivity Sensor into an Animal-borne Instrument

    DTIC Science & Technology

    2014-09-30

    animal -borne instrument Lars Boehme Sea Mammal Research Unit Scottish Oceans Institute University of St Andrews St Andrews, KY16 8LB United... Kingdom phone: +44 1334-462677 fax: +44 1334-463443 email: lb284@st-andrews.ac.uk Robin Pascal Sensors Development Group National...Oceanography Centre Southampton, SO14 3ZY United Kingdom phone: +44 2380-596138 fax: +44 2380-593029 email: rwp@nerc.ac.uk Phil Lovell SMRU

  2. 76 FR 73683 - Whirlpool Corporation, Including On-Site Leased Workers From Career Solutions TEC Staffing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... Solutions TEC Staffing, Andrews International, IBM Corporation, TEK Systems Penske Logistics, Eurest... Systems, Penske Logistics, Eurest, Canteen, Kelly Services, Inc., Prodriver, and Arkansas Warehouse,...

  3. 77 FR 26477 - Receipt of a Pesticide Petition Filed for Residues of Pesticide Chemicals in or on Various...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... INFORMATION CONTACT: Andrew Bryceland, Biopesticides and Pollution Prevention Division (7511P), Office of... Director, Biopesticides and Pollution Prevention Division, Office of Pesticide Programs. BILLING CODE...

  4. 78 FR 45535 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ..., Tampa, Florida; William Andrew Krusen, Jr. SEP IRA, Tampa, Florida; Krusen Limited Partnership, Ltd.... Thompson Krusen, Tampa, Florida; WIT Financial Group, LLC, Tampa, Florida; and WIT Ventures, Ltd.,...

  5. 40 CFR 81.437 - New Brunswick, Canada.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...* Treat's Island* Passamaquoddy Bay* Deer Island* Indian Island* Rouen Island* Cherry Island* Thrumcap...* Deer Island* Passamaquoddy Bay* Old Sow Whirlpool* St. Andrews* Eastport* Friar Roads* Estes...

  6. 40 CFR 81.437 - New Brunswick, Canada.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...* Treat's Island* Passamaquoddy Bay* Deer Island* Indian Island* Rouen Island* Cherry Island* Thrumcap...* Deer Island* Passamaquoddy Bay* Old Sow Whirlpool* St. Andrews* Eastport* Friar Roads* Estes...

  7. 40 CFR 81.437 - New Brunswick, Canada.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...* Treat's Island* Passamaquoddy Bay* Deer Island* Indian Island* Rouen Island* Cherry Island* Thrumcap...* Deer Island* Passamaquoddy Bay* Old Sow Whirlpool* St. Andrews* Eastport* Friar Roads* Estes...

  8. 40 CFR 81.437 - New Brunswick, Canada.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...* Treat's Island* Passamaquoddy Bay* Deer Island* Indian Island* Rouen Island* Cherry Island* Thrumcap...* Deer Island* Passamaquoddy Bay* Old Sow Whirlpool* St. Andrews* Eastport* Friar Roads* Estes...

  9. STS-134 Tribute to Endeavour

    NASA Video Gallery

    STS-134 Commander Mark Kelly pays tribute to space shuttle Endeavour and the spacecraft's contribution to human spaceflight. Mission specialists Andrew Feustel, Mike Fincke, Roberto Vittori, Greg C...

  10. E-Books Mediator: Nicholas Bogaty--Open Ebook Forum, New York

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    This article is about the work of Nick Bogaty, executive director of the Open eBook Forum. Nick Bogaty is not a librarian, but he plays nicely with them, along with publishers, hardware manufacturers, software producers, database vendors, and disability rights advocates. All are groups that share an interest in making e-books work for their…

  11. Towards a Formalism for Program Generation 1992 - Final Report

    DTIC Science & Technology

    1992-12-01

    Contributors: Raymond Yeh, Peter Ng, Luqi, Joseph Urban, Ron Norman, W.D. Hurley, John Baker, Patrick Bobbie, WT Tsai, Greg Boone, Nick Bourbakis , etc. In...Ltd. Contributors: Raymond Yeh, Peter Ng, Luqi, Joseph Urban, Ron Norman, W.D. Hurley, John Baker, Patrick Bobbie, WT Tsai, Greg Boone, Nick Bourbakis

  12. Accentuating the Positive: "Can't" Doesn't Cut It

    ERIC Educational Resources Information Center

    Hyndman, Amy

    2011-01-01

    Nick is the author's first-born child. He was born healthy in 1993. Within days, however, there would be medical complications resulting in brain damage, seizures, medical issues, a diagnosis of cerebral palsy at four months of age, and later, a diagnosis of bi-lateral progressive hearing loss at eight years of age. Nick's parents have been told…

  13. 78 FR 7645 - Airworthiness Directives; Bell Helicopter Textron, Inc., Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... (FPI) of each crosstube and upper center support for a crack, any corrosion, nick, scratch, dent, or... conducted as a result of recent field failures and corrosion problems of the affected crosstubes. The... crosstube and upper center support for a crack, any corrosion, a nick, scratch, dent, or other...

  14. Analysis of the Latin Square Task with Linear Logistic Test Models

    ERIC Educational Resources Information Center

    Zeuch, Nina; Holling, Heinz; Kuhn, Jorg-Tobias

    2011-01-01

    The Latin Square Task (LST) was developed by Birney, Halford, and Andrews [Birney, D. P., Halford, G. S., & Andrews, G. (2006). Measuring the influence of cognitive complexity on relational reasoning: The development of the Latin Square Task. Educational and Psychological Measurement, 66, 146-171.] and represents a non-domain specific,…

  15. Technology Counts 2007: A Digital Decade

    ERIC Educational Resources Information Center

    Education Week, 2007

    2007-01-01

    "Technology Counts 2007" looks back, and ahead, after a decade of enormous upheaval in the educational technology landscape. This special issue of "Education Week" includes the following articles: (1) A Digital Decade; (2) Getting Up to Speed (Andrew Trotter); (3) E-Rate's Imprint Seen in Schools (Andrew Trotter); (4) Teaching…

  16. Characterization of Marine Mammal Recordings from the Hawaii Range Complex

    DTIC Science & Technology

    2010-11-01

    Germany. 100 pp. Baumann-Pickering, S., S. M. Wiggins, E. Roth , M. A. Roch, H.-U. Schnitzler, and J. A. Hildebrand. (2010). “Echolocation...Institution Woods Hole, MA Ian Boyd University of St. Andrews St. Andrews, Scotland , UK 1 1 1 1 1

  17. Huggins, martians and exoplanets

    NASA Astrophysics Data System (ADS)

    2013-02-01

    The RAS Ordinary A & G meeting in November included the Gerald Whitrow Lecture by Prof. Andrew Liddle, while in December Prof. Andrew Collier Cameron gave the George Darwin Lecture. Both will be reported in future issues. Here Sue Bowler summarizes the other talks.

  18. 77 FR 17478 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ..., Illinois 60690-1414: 1. Steven D. Spector, Glenview, Illinois, as an individual and as trustee of the Alan L. Spector GST Family Trust, the Walter W. Spector, Steven D. Spector, Andrew M. Spector, and Nancy... with Steven D. Spector, Walter W. Spector Saratoga, California, Andrew M. Spector, Bexley, Ohio,...

  19. Root Cause Investigation (RCI) Best Practices Guide Product Overview

    DTIC Science & Technology

    2014-05-08

    micropac.com Andrew King Boeing andrew.m.king@boeing.com Byron Knight NRO knightby@nro.mil Hans Koenigsmann SpaceX hans.koenigsmann@spacex.com James Koory...ghislain.turgeon@sslmda.com Deborah Valley MIT deborah.valley@ll.mit.edu Fred Van Milligen JDSU fvanmilligen@jdsu.com Marvin VanderWeg SpaceX marvin.vanderwag

  20. Standard/Handbook for Multipactor Breakdown Prevention in Spacecraft Components

    DTIC Science & Technology

    2014-05-28

    micropac.com Micopac Andrew King andrew.m.king@boeing.com Boeing Byron Knight knightby@nro.mil NRO Hans Koenigsmann hans.koenigsmann@spacex.c om SpaceX ...VanderWeg marvin.vanderwag@spacex.c om SpaceX Gerrit VanOmmering gerrit.vanommering@sslmda. com SSL Michael Verzuh mverzuh@ball.com Ball John Vilja

  1. Guidelines for Hosted Payload Integration

    DTIC Science & Technology

    2014-06-06

    Micopac markking@micropac.com Andrew King Boeing andrew.m.king@boeing.com Byron Knight NRO knightby@nro.mil Hans Koenigsmann SpaceX hans.koenigsmann...jdsu.com Marvin VanderWeg SpaceX marvin.vanderwag@spacex.c om Gerrit VanOmmering SSL gerrit.vanommering@sslmda. com Michael Verzuh Ball mverzuh@ball.com

  2. Counterfeit Parts Prevention Strategy Guide Product Overview

    DTIC Science & Technology

    2014-05-08

    pwr.utc.com Mark King Micopac markking@micropac.com Andrew King Boeing andrew.m.king@boeing.com Byron Knight NRO knightby@nro.mil Hans Koenigsmann SpaceX ...Marvin VanderWeg SpaceX marvin.vanderwag@spacex.com Gerrit VanOmmering SSL gerrit.vanommering@sslmda.com Michael Verzuh Ball mverzuh@ball.com John Vilja

  3. CrossTalk: The Journal of Defense Software Engineering. Volume 27, Number 5, September/October 2014

    DTIC Science & Technology

    2014-10-01

    components. Other work has been done by Young, 1991. Also other work has been done by Andrew Rae , and Colin Fidge [26] to improve Young’s approach by...26. Andrew Rae , Colin Fidge, “Identifying Critical Components During Information Security Evaluation” CrossTalk—September/October 2014 35

  4. Increase of Breakthrough Pressure of Cotton Fabric by Fluoropolymer/Fluoroposs Treatment

    DTIC Science & Technology

    2011-11-29

    Fluoropolymer/Fluoroposs 5b. GRANT NUMBER Treatment 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Kevin R. Lamison, Andrew J. Guenthner , and Joseph M. Mabry 5d...Fabric by l-’luoropolymer/1əuoroposs Treatment Kevin R Lamison’. Andrew.! Guenthner , Joseph M Mabr;l ’ERC Incorporated Edwards AFB, CA 93524

  5. 78 FR 894 - Interim Final Determination To Stay Sanctions, Imperial County Air Pollution Control District

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... AGENCY 40 CFR Part 52 Interim Final Determination To Stay Sanctions, Imperial County Air Pollution... of revisions to the Imperial County Air Pollution Control District (ICAPCD) portion of the California...- line instructions. 2. Email: steckel.andrew@epa.gov . 3. Mail or deliver: Andrew Steckel (Air-4),...

  6. Creating a Shared Definition of Good and Bad Writing through Revision Strategies

    ERIC Educational Resources Information Center

    Baer, Allison L.

    2008-01-01

    This article describes how the author, in an effort to build some common language about "good writing" and to teach effective revision strategies, involved her students in an action research project using the Worse/Better Writing Strategy developed by Andrews-Beck. In her dissertation, Andrews-Beck described a worse/better writing strategy that…

  7. Treemaps as a Tool for Social Network Analysis

    DTIC Science & Technology

    2005-09-01

    Andrews & Heidegger , 1998) over the decade since its origin. The idea was initially conceived to simplify the difficulty of managing file space on a...442, New York City, New York, USA, September 29 - October 2, 2004. Springer-Verlag. Andrews, K., & Heidegger , H. (1998). Information slices

  8. Accountable Information Flow for Java-Based Web Applications

    DTIC Science & Technology

    2010-01-01

    Attendees: Andrew Myers. 10. IBM PL Day, May 7, Hawthorne , New York. Presented keynote talk on the Fabric secure persistence layer: “Fabric: a...Languages (POPL), pages 228–241, January 1999. [14] Andrew C. Myers, Lantian Zheng, Steve Zdancewic, Stephen Chong, and Nathaniel Nys- trom. Jif 3.0

  9. Coalition Operations in Afghanistan Post 2014

    DTIC Science & Technology

    2013-02-14

    part of the world. Notes 1 Andrew Wegener , A Complex and Changing Dynamic: Afghan...29-60. Rust, Stephen N. “The Nuts and Bolts of Village Stability Operations.” Special Warfare. (Jul-Sep 2011): 28-31. Wegener , Andrew. A

  10. 77 FR 25954 - Receipt of Several Pesticide Petitions Filed for Residues of Pesticide Chemicals in or on Various...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ... Chemistry Laboratory (ACL). Contact: Andrew Ertman, (703) 308-9367, email address: ertman.andrew@epa.gov . 4...-25958] [FR Doc No: 2012-10321] ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 180 [EPA-HQ-OPP-2012-0001... Various Commodities AGENCY: Environmental Protection Agency (EPA). ACTION: Notice of filing of...

  11. 77 FR 11476 - Delegation of National Emission Standards for Hazardous Air Pollutants for Source Categories; Nevada

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-27

    ....andrew@epa.gov . 3. Mail or deliver: Andrew Steckel (AIR-4), U.S. Environmental Protection Agency Region... for this action is available electronically at www.regulations.gov and in hard copy at EPA Region IX... index, some information may be publicly available only at the hard copy location (e.g.,...

  12. Why Play "I spy" When You Can Do Mathematics?

    ERIC Educational Resources Information Center

    Andrews, Robert; Andrews, Paul

    2007-01-01

    This article presents a story of Robert Andrew's mental engagement with mathematics. It has been jointly written by Robert, who is a Y8 student, and his father, Paul Andrews, who is a mathematics educator. It starts with Robert who presents the context for the story and is followed by Paul's commentary which draws on what Robert has written.…

  13. Decompression Sickness Risk Versus Time and Altitude

    DTIC Science & Technology

    1993-11-01

    M, Wiegman J, Pilmanis exposure at 22,500 ft (Fig. 1-2) should raise AA. Prebreathe enhancement with concern since that level of decompression is...Andrew A. P11manis in a research phyniologist 2. Fischer MO, Wiegman JF, McLean SA, Olson Andrew A. ihi ish resiarch poycioo SRM. Evaluation of four

  14. Personal Ethics versus Professional Ethics

    DTIC Science & Technology

    1996-01-01

    Colorado Springs, Colo.: Navpress, 1978). 2. Quoted in Andrew Stark, “What’s the Matter with Business Ethics?” Harvard Business Review , May–June 1993...39. 3. Ibid., 40. 4. Kenneth R. Andrews, “Ethics in Practice,” Harvard Business Review , September–October 1989, 99. 5. Quoted in Perspective: A

  15. An Investigation into the Relationship between Ionospheric Scintillation and Loss of Lock in GNSS Receivers

    DTIC Science & Technology

    2006-06-01

    and Loss of Lock in GNSS Receivers Robert W. Meggs, Cathryn N. Mitchell and Andrew M . Smith Department of Electronic and Electrical Engineering...into the relationship between ionospheric scintillation and loss of lock in GNSS receivers Robert W. Meggs, Cathryn N. Mitchell and Andrew M . Smith Department

  16. Network-Based Approach to Optimize Personnel Recovery for the Joint Force

    DTIC Science & Technology

    2011-05-26

    NUMBER Andrew M . Smith , Major, USAF 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES...NETWORK-BASED APPROACH TO OPTIMIZE PERSONNEL RECOVERY FOR THE JOINT FORCE by Andrew M . Smith Major, USAF A paper submitted to the

  17. Molecular Mechanisms and Treatment Strategies for Obesity-Associated Coronary Artery Disease, an Imminent Military Epidemic

    DTIC Science & Technology

    2008-12-01

    lesions that lacked nuclei and cytoplasm. In Situ TdT-Mediated dUTP Nick -End Labeling Assays Apoptotic cells in the intima of atherosclerotic lesions...were detected by the TdT-mediated dUTP nick -end labeling (TUNEL) technique using the TMR red in situ cell death detection kit (Roche Diagnos- tics...data were verified by TUNEL (Tdt-mediated dUTP nick end labeling) analysis, using the in-situ cell death detection kit, TMR red, from Roche (see

  18. Air University Library Index to Military Periodicals, Volume 43, Number 3, July-September 1992,

    DTIC Science & Technology

    1992-09-01

    Moore. illus por. Military RETIRF.MENT - Air Force for the few. Nick Cook. ’Ilus tab. Jane’s EnA ineer 84 no 551 (Aug 󈨠): p 16 -17 ROLES AND...Russian multi-role MiG-33 revealed. Nick United States Airlift: Lifeline of the warriors. Dane Cook. illus. Jane’s Dlefence Weekly 17 nt, I ivd C Ste-arman...I no 2 (Jul 󈨠): p4-7 12 (Sep 19 󈨠): pl 7 -18+ IF-22 GAO faults B-52 performance in Gulf. Neff Russian multi-role MiG-33 revealed. Nick New fighter

  19. Targeting Stat3 With G-Quartet Oligonucleotides in Metastatic Prostate Cancer

    DTIC Science & Technology

    2005-04-01

    Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick Densmore (Baylor College of Medicine). Interleukin (IL) 6 and antibodies End-Labeling Assay. The...terminal deoxynucleotidyl transferase-mediated against Stat3, StatI, Bcl-XL, and Bcl-2 were purchased from Santa Cruz dUTP-biotin nick end-labeling...mediated dUTP-biotin nick end-labeling (TUNEL) injected into the tail vein of male and female mice and rats. was performed as an assay for apoptosis and

  20. Real-Time Detection of Telomerase in a Microelectromechanical Systems Platform

    DTIC Science & Technology

    2005-05-01

    plasmid (Figure 27B, 3558 bp). Lane C contained the uncut pGRN83 (Figure 27C). The uncut plasmid was nicked and therefore migrated at a slower...in this lane. The first and most intense band which is above the 1kb ladder is the plasmid but the plasmid is in a nicked state. The topography...distorted because of the physical characteristics of the plasmid. The plasmids were in a nicked state, i.e., they appeared much larger then they should

  1. Critical bending torque of DNA is a materials parameter independent of local base sequence

    NASA Astrophysics Data System (ADS)

    Wang, Juan; Qu, Hao; Zocchi, Giovanni

    2013-09-01

    Short double-stranded DNA molecules exhibit a softening transition under large bending which is quantitatively described by a critical bending torque τc at which the molecule develops a kink. Through equilibrium measurements of the elastic energy of short (˜10 nm), highly stressed DNA molecules with a nick at the center we determine τc for different sequences around the nick. We find that τc is a robust materials parameter essentially independent of sequence. The measurements also show that, at least for nicked DNA, the local structure at the origin of the softening transition is not a single-stranded “bubble.”

  2. Evaluating the U.S. Military’s Development of Strategic and Operational Doctrine for Non-Lethal Weapons in a Complex Security Environment

    DTIC Science & Technology

    2007-11-02

    Another book by Nick Lewer and Steven Schofield, Non-Lethal Weapons: A Fatal Attraction?8, expresses concern about the development and misuse of...of weapons and use military-style tactics and operational behavior.”9 Nick Lewer also edited a more recent book, The Future of Non-Lethal Weapons...Weapons,” The Future of Non-Lethal Weapons, edited by Nick Lewer (Portland 2002), 46. 84CSIS, “Non-Lethal Weapons Policy Study,” p. 12. 85Ibid

  3. Adenosine-guanosine preferential photocleavage of DNA by azido-benzoyl- and diazocyclopenta-dienylcarbonyloxy derivatives of 9-aminoacridine.

    PubMed Central

    Nielsen, P E; Jeppesen, C; Egholm, M; Buchardt, O

    1988-01-01

    The photoreactions of 9-[6-(4-azidobenzamido)hexylamino]acridine (AHA) and 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) with double stranded DNA result in formation of single strand nicks and alkali labile sites (adducts) with an efficiency of 6 x 10(-3) nicks per AHA and 3 x 10(-2) nicks per DHA molecule. The alkali dependent DNA cleavage by AHA shows a pronounced A+G preference whereas that by DHA is practically sequence independent. In the presence of diacridines, however, DHA exhibits a preference for cleavage at guanosines. These DNA photocleaving reagents could be useful for DNA photofootprinting and photosequencing. Images PMID:2836803

  4. Hurricane impacts on the coastal environment

    USGS Publications Warehouse

    Sallenger, Abby

    1990-01-01

    In terms of insured losses, Hurricane Andrew is the most severe catastrophe in the Nation's history. Prior to the arrival of Andrew, the U.S. Geological Survey (USGS), in cooperation with the Louisiana Geological Survey (LGS), acquired an extensive body of information and data on the behavior and long-term erosion of Louisiana barrier islands. As a result, we have a clear understanding of pre-storm conditions in this area; Andrew provided an opportunity to learn in detail the impact of a very large storm on Louisiana coastal environment.

  5. Department of Defense High Performance Computing Modernization Program. 2007 Annual Report

    DTIC Science & Technology

    2008-03-01

    management services for Current and Future Force Battle Command systems throughout all phases of operations and environments. Harley Cudney (ERDC...HPCMP Annual Report 2007 84 Keynote speakers included: Brigadier General Nick Justice, Deputy Program Executive Officer, Army Command, Control

  6. 14 CFR Appendix A to Part 43 - Major Alterations, Major Repairs, and Preventive Maintenance

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... beams. (vii) Thin sheet webs of beams. (viii) Keel and chine members of boat hulls or floats. (ix... controllable pitch propellers. (xiii) Repairs to deep dents, cuts, scars, nicks, etc., and straightening...

  7. 14 CFR Appendix A to Part 43 - Major Alterations, Major Repairs, and Preventive Maintenance

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... beams. (vii) Thin sheet webs of beams. (viii) Keel and chine members of boat hulls or floats. (ix... controllable pitch propellers. (xiii) Repairs to deep dents, cuts, scars, nicks, etc., and straightening...

  8. 14 CFR Appendix A to Part 43 - Major Alterations, Major Repairs, and Preventive Maintenance

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) Spar flanges. (vi) Members of truss-type beams. (vii) Thin sheet webs of beams. (viii) Keel and chine...) Repairs to deep dents, cuts, scars, nicks, etc., and straightening of aluminum blades. (xiv) The repair...

  9. 14 CFR Appendix A to Part 43 - Major Alterations, Major Repairs, and Preventive Maintenance

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... beams. (vii) Thin sheet webs of beams. (viii) Keel and chine members of boat hulls or floats. (ix... controllable pitch propellers. (xiii) Repairs to deep dents, cuts, scars, nicks, etc., and straightening...

  10. 14 CFR Appendix A to Part 43 - Major Alterations, Major Repairs, and Preventive Maintenance

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... beams. (vii) Thin sheet webs of beams. (viii) Keel and chine members of boat hulls or floats. (ix... controllable pitch propellers. (xiii) Repairs to deep dents, cuts, scars, nicks, etc., and straightening...

  11. 77 FR 17107 - Qualification of Drivers; Exemption Applications; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-23

    ... (GA) Curtis J. Crowston (ND) Clifford H. Dovel (WA) Arthur L. Fields (SC) Rupert G. Gilmore, III (AL... (KY) Travis J. Luce (MI) Jack D. Miller (OH) Eric M. Moats, Sr. (MD) Robert W. Nicks (NY) Joseph...

  12. 78 FR 60182 - Airworthiness Directives; Bell Helicopter Textron, Inc., Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ... record for each spindle, inspecting the spindles for any corrosion, or a nick, scratch, dent, or crack... AD defines the unsafe condition as fatigue cracking of a spindle. This condition could result...

  13. 78 FR 20942 - Niobrara Confluence and Ponca Bluffs Conservation Areas, NE and SD; Draft Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-08

    ..., Division of Refuge Planning, P.O. Box 25486, DFC, Denver, CO 80225. FOR FURTHER INFORMATION CONTACT: Nick.... Box 25486, DFC, Denver, CO 80225. SUPPLEMENTARY INFORMATION: Introduction With this notice,...

  14. A multiverse play divides opinion

    NASA Astrophysics Data System (ADS)

    Crease, Robert P.

    2015-03-01

    The stage lights rise. A man and woman meet in a cute way - "Do you know why it's impossible to lick the tips of your elbows?" she asks - they chat momentarily, and separate. The play is Constellations by Nick Payne.

  15. Policing in Afghanistan-Reform that Respects Tradition: Need for a Strategic Shift

    DTIC Science & Technology

    2010-05-01

    118. 35 Nathan Hodge , “Army Researchers: Why the Kandahar Offensive Could Backfire,” Danger Room (16 April 2010), www.wired.com/dangerroom/2010/04...can win the war and lose the battle at home.38 Nick Grono, the Deputy President for Operations of the International Crisis Group, Brussels, has also...graduates re- 40 Nick Grono, “Policing in Conflict Studies – Lessons from Afghanistan,” International

  16. Removing the Rose Colored Glasses: Exploring Modern Security Environment’s Effect on the Army Assignment Policy for Women

    DTIC Science & Technology

    2009-06-12

    functioning as intended in the current deployed environment. Next, Mr. Harold Hodges and Ms. Andrea Auvil-Jones from the Training and Doctrine Command...media outlet, USA Today covered these stories including the beheading of the American, Nick Berg, which showed part of the agenda of insurgents and...and government, and coalition partners. The beheading of American freelance, telecommunications contractor Nick Berg in Iraq and the resulting

  17. 2011 JSOU and NDIA SO/LIC Division Essays

    DTIC Science & Technology

    2011-07-01

    marines.org/gazette/article/marine-corps- intelligence-operations-anbar). 4. Hope Hodge , “Marines look to improve deployment-to-dwell ratios.” Jdnews...5. W.F. Lawless, Margo Bergman, J. Lou, Nicole Kriegel, and Nick Feltovich, “A Quantum Metric for Organizational Performance: Terrorism and...Dinsha Mistree, Stuart E. Mad- nick , Jay B. Morrison, Michael D. Siegel, Margaret Sweitzer-Hamilton, “Under- standing & Modeling State Stability

  18. Churchill: The Making of a Grand Strategist

    DTIC Science & Technology

    1990-01-01

    twilight war.27 As for the army, its arrival during the Marne campaign, Churchill added, "reached in the nick of time the vital post on the flank of the...sudden surprising arrival of Eugene to rescue the coalition commander at Blenheim in the nick of time, 30 as had sometimes been reported until...pressured the government to impeach the second of his enemies, Robert Harley , Earl of Oxford and former Lord Treasurer. Ashley, p. 156. 114. Wheeler

  19. Warfare in the 21st Century. A Selected Bibliography

    DTIC Science & Technology

    2003-07-01

    Grier, Peter. "The Sensational Signal." (Global Positioning System) Air Force Magazine 86 (Feb- ruary 2003): 66-69. Harley , Jeff. "Space Control and...Life-Saving Heli- copters." Armed Forces Journal 140 (May 2003): 42-48. Cook, Nick . "Armed and Dangerous." (Unmanned Combat Aerial Vehicles) Jane’s...www.au.af.mil/au/aul/bibs/soft/nonlethal.htm>. Internet. Accessed 19 May 2003. Lewer, Nick , ed. The Future of Non-lethal Weapons: Technologies

  20. Technology Insertion-Engineering Services Process Characterization, Task Order Number 1. Volume 5. Revision A

    DTIC Science & Technology

    1989-12-22

    titled, "To Reduce End Item Assembly lime," recommends that visual inspection, nick , and deburring of hardware items which are presently done in MATPSS...improvements in process quality at the Harley - Davidson Corp. plant (which undertook a similar, though more extensive, restructuring in 1986) were so...during FY 88. Of these, 20-30% (estimated) received only a visual inspection, nick and burr removal, and corrosion treatment (no dimensional inspection

  1. The Interplay Between Estrogen and Replication Origins in Breast Cancer DNA Amplification

    DTIC Science & Technology

    2014-11-01

    have been processed at Nick Rhind’s laboratory (Department of Biochemistry and Molecular Pharmacology , University of Massachusetts Medical School...Organization Name: Nick Rhind’s laboratory, Department of Biochemistry and Molecular Pharmacology , University of Massachusetts Medical School... Nurse P. Specific replication origins promote DNA amplification in fission yeast. J Cell Sci. 2010; 123(Pt 18):3047-51. Liew GM, Foulk MS, Gerbi SA. The

  2. Optimizing the Domestic Chemical, Biological, Radiological, and Nuclear Response Enterprise

    DTIC Science & Technology

    2015-03-01

    OPTIMIZING THE DOMESTIC CHEMICAL , BIOLOGICAL, RADIOLOGICAL, AND NUCLEAR RESPONSE ENTERPRISE THESIS MARCH 2015 Nick Paul, Captain, USA AFIT-ENS-MS-15...of the U.S. Government and is not subject to copyright protection in the United States. AFIT-ENS-MS-15-M-143 OPTIMIZING THE DOMESTIC CHEMICAL ...DISTRIBUTION UNLIMITED. AFIT-ENS-MS-15-M-143 OPTIMIZING THE DOMESTIC CHEMICAL , BIOLOGICAL, RADIOLOGICAL, AND NUCLEAR RESPONSE ENTERPRISE THESIS Nick Paul

  3. The Limited Utility of Non-Lethal Weapons in Decisive Combat: War Still Means Fighting and Fighting Still Means Killing

    DTIC Science & Technology

    2005-05-17

    Navy, The Commander’s Handbook on the Law of Naval Operations, NWP 1-14M (Washington, DC: 1995): para 11.4. 22 Nick Lewer and Neil Davidson...February 2003). Lewer, Nick , Davidson, Neil. “Non-Lethal Technologies—An Overview.” Disarmament Forum. (2005): 37-51...Summer 2004), 24-32. Sautenett, Vincent. “Legal Issues Concerning Military Use of Non-Lethal Weapons.” Murdoch University Electronic Journal of

  4. "Who's There?": Shakespeare and the Dragon of Autism

    ERIC Educational Resources Information Center

    Renino, Christopher

    2009-01-01

    Dan recently turned 13; Nick will soon be 12. Both boys are smart and fun; both are loving, write like angels, like to learn, and want friends; both are autistic and hope to learn to speak. They find it challenging to relate to and function in this world, and they work hard to improve their abilities to do so. Last fall, Nick and Dan became…

  5. The Future of Theoretical Physics and Cosmology

    NASA Astrophysics Data System (ADS)

    Gibbons, G. W.; Shellard, E. P. S.; Rankin, S. J.

    2009-08-01

    Preface; List of contributors; 1. Introduction; Part I. Popular Symposium: 2. Our complex cosmos and its future Martin J. Rees; 3. Theories of everything and Hawking's wave function of the Universe James B. Hartle; 4. The problem of space-time singularities: implications for quantum gravity? Roger Penrose; 5. Warping spacetime Kip Thorne; 6. 60 years in a nutshell Stephen W. Hawking; Part II. Spacetime Singularities: 7. Cosmological perturbations and singularities George F. R. Ellis; 8. The quantum physics of chronology protection Matt Visser; 9. Energy dominance and the Hawking-Ellis vacuum conservation theorem Brandon Carter; 10. On the instability of extra space dimensions Roger Penrose; Part III. Black Holes: 11. Black hole uniqueness and the inner horizon stability problem Werner Israel; 12. Black holes in the real universe and their prospects as probes of relativistic gravity Martin J. Rees; 13. Primordial black holes Bernard Carr; 14. Black hole pair creation Simon F. Ross; 15. Black holes as accelerators Steven Giddings; Part IV. Hawking Radiation: 16. Black holes and string theory Malcolm Perry; 17. M theory and black hole quantum mechanics Joe Polchinski; 18. Playing with black strings Gary Horowitz; 19. Twenty years of debate with Stephen Leonard Susskind; Part V. Quantum Gravity: 20. Euclidean quantum gravity: the view from 2002 Gary Gibbons; 21. Zeta functions, anomalies and stable branes Ian Moss; 22. Some reflections on the status of conventional quantum theory when applied to quantum gravity Chris Isham; 23. Quantum geometry and its ramifications Abhay Ashtekar; 24. Topology change in quantum gravity Fay Dowker; Part VI. M Theory and Beyond: 25. The past and future of string theory Edward Witten; 26. String theory David Gross; 27. A brief description of string theory Michael Green; 28. The story of M Paul Townsend; 29. Gauged supergravity and holographic field theory Nick Warner; 30. 57 varieties in a NUTshell Chris Pope; Part VII. de Sitter Space

  6. Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states.

    PubMed

    Cotner-Gohara, Elizabeth; Kim, In-Kwon; Hammel, Michal; Tainer, John A; Tomkinson, Alan E; Ellenberger, Tom

    2010-07-27

    Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a "jackknife model" in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

  7. Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching between Two DNA-Bound States

    SciTech Connect

    Cotner-Gohara, Elizabeth; Kim, In-Kwon; Hammel, Michal; Tainer, John A.; Tomkinson, Alan E.; Ellenberger, Tom

    2010-09-13

    Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a 'jackknife model' in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

  8. 76 FR 25301 - Foreign-Trade Zone 141-County of Monroe, New York; Application for Reorganization Under...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-04

    ..., Kathleen Boyce of the FTZ Staff is designated examiner to evaluate and analyze the facts and information..., contact Kathleen Boyce at Kathleen.Boyce@trade.gov or (202) 482-1346. Dated: April 28, 2011. Andrew...

  9. 77 FR 17408 - Foreign-Trade Zone 204-Tri-Cities Area, TN/VA; Application for Reorganization Under Alternative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... no impact on FTZ 204's authorized subzone. In accordance with the Board's regulations, Kathleen Boyce... Kathleen Boyce at Kathleen.Boyce@trade.gov or (202) 482-1346. Dated: March 20, 2012. Andrew...

  10. Advanced Materials and Solids Analysis Research Core (AMSARC)

    EPA Science Inventory

    The Advanced Materials and Solids Analysis Research Core (AMSARC), centered at the U.S. Environmental Protection Agency's (EPA) Andrew W. Breidenbach Environmental Research Center in Cincinnati, Ohio, is the foundation for the Agency's solids and surfaces analysis capabilities. ...

  11. 77 FR 62216 - Proposed Foreign-Trade Zone-Eloy, AZ Comment Period on New Evidence

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-12

    ... evidence is open through November 13, 2012. Submissions shall be addressed to the Board's Executive...: October 5, 2012. Andrew McGilvray, Executive Secretary. [FR Doc. 2012-25166 Filed 10-11-12; 8:45...

  12. 77 FR 54927 - Comment Request for Information Collection for Unemployment Insurance (UI) Benefit Accuracy...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... Employment and Training Administration Comment Request for Information Collection for Unemployment Insurance... written comments to Andrew Spisak, Office of Unemployment Insurance, Room S-4524, Employment and Training... UI benefit payments in three programs: State UI, Unemployment Compensation for Federal...

  13. 76 FR 72978 - Whirlpool Corporation Including On-Site Leased Workers From Career Solutions TEC Staffing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... Solutions TEC Staffing, Andrews International, IBM Corporation, TEK Systems, Penske Logistics, Eurest, and.... The company reports that workers leased from IBM Corporation, TEK Systems, Penske Logistics, Eurest... to include workers leased from IBM Corporation, TEK Systems, Penske Logistics, Eurest, and...

  14. Aiming high but keeping costs low.

    PubMed

    Hough, Steve; Nicholls, Andrew

    2010-04-01

    Steve Hough, national sales manager, CEP Claddings, and Andrew Nicholls, sales director, CEP Ceilings, explain how modern cladding and ceiling materials can enhance the appearance of modern hospital buildings while offering practical, cost-saving, and environmental advantages.

  15. 7 CFR 319.77-3 - Gypsy moth infested areas in Canada.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... Andrews, St. Croix, St. David, St. George, St. James, St. Patrick, and St. Stephen. (2) Kings County. That... District that includes the City of Sudbury and the townships of Baldwin, Dryden, Dunlop, Graham,...

  16. Questions to Ask Your Doctor

    MedlinePlus

    ... the News Advocacy Group Helps Patients Cope 15 Mar 2017 MBCN Selects University of Pittsburgh Cancer Institute’s ... 000 Metastatic Breast Cancer Research Leadership Award 08 Mar 2017 MBCN Selects Johns Hopkins’ Dr. Andrew Ewald ...

  17. Webb Telescope Backplane Arrives at NASA Goddard Space Flight Center

    NASA Video Gallery

    Webb Telescope's Backplane arrived at Joint Base Andrews on Monday, August 24, 2015 aboard a U.S. Air Force C-5 cargo plane. The Backplane, inside the Space Telescope Transporter for Air Road and S...

  18. RNA interference: unraveling a mystery.

    PubMed

    Montgomery, Mary K

    2006-12-01

    Andrew Fire and Craig Mello have won the Nobel Prize in Medicine or Physiology for their discovery of RNA interference. Mary K. Montgomery, then a postdoc in the Fire laboratory, participated in some of the key experiments.

  19. The (nano) entrepreneur's dilemma

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2015-03-01

    Emerging technologies need to be developed responsibly if their benefits are to outweigh any potential risks. Yet do entrepreneurs really have the luxury of grappling with future consequences from the get-go, asks Andrew D. Maynard.

  20. 2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 FACING NORTHWEST. NOTE CURVED KNEE-BRACES ON SUPPORT POSTS. - Medford Service Center, Covered Storage Building, 1319 McAndrews Road, Medford, Jackson County, OR

  1. Breast skin and nipple changes

    MedlinePlus

    ... treatment. SCALY, FLAKING, ITCHY SKIN This is usually eczema or a bacterial or fungal infection. See your ... Atopic dermatitis, eczema, and noninfectious immunodeficiency disorders. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the Skin: Clinical ...

  2. 78 FR 32553 - Establishment of Class E Airspace; Boothbay, ME

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ..., Georgia 30320; telephone (404) 305-6364. SUPPLEMENTARY INFORMATION: History On March 28, 2013, the FAA... feet or More Above the Surface of the Earth. * * * * * ANE ME E5 Boothbay, ME St. Andrews...

  3. 3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING #2205 (AREA CONTAINING VEHICLE REPAIR SHOP AND VEHICLE MANAGEMENT OFFICE), FACING SOUTHWEST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  4. 78 FR 33394 - Notice To Extend Submittal Date for Scoping Comments for United States Air Force Main Operating...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-04

    ... scoping period will be considered in the preparation of the Draft EIS. Point of Contact: Please direct any.../A7AM, 3501 Fetchet Avenue, Joint Base Andrews, Maryland, 20762- 5157. Henry Williams Jr., Acting...

  5. Saved by Bell

    NASA Astrophysics Data System (ADS)

    Gisin, Nicolas

    2017-01-01

    In John Stewart Bell and Twentieth-Century Physics: Vision and Integrity, fellow physicist Andrew Whitaker tells the story of Bell's life and his revolutionary discovery that not everything in physics can be explained using only local variables.

  6. Effects of Disasters: Risk and Resilience Factors

    MedlinePlus

    ... caused and natural disasters. Disasters may be explosions, earthquakes, floods, hurricanes, tornados, or fires. In a disaster, ... at severe natural disasters, such as the Armenian earthquake, mudslides in Mexico, and Hurricane Andrew in the ...

  7. Magnetotails throughout the solar system

    NASA Astrophysics Data System (ADS)

    Forsyth, C.; Milan, S. E.; Walsh, A. P.

    2010-12-01

    MEETING REPORT A key part of heliospheric and planetary physics came under scrutiny at an RAS Specialist Discussion Meeting in October. Colin Forsyth, Chris Arridge, Steve Milan and Andrew Walsh summarize.

  8. Pityriasis rubra pilaris

    MedlinePlus

    ... Pityriasis rubra pilaris - close-up References James WD, Berger TG, Elston DM. Pityriasis rosea, pityriasis rubra pilaris, ... other papulosquamous and hyperkeratotic diseases. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the ...

  9. Radiation from Cardiac Imaging Tests

    MedlinePlus

    ... thought you would like to see the Circulation web site. Your Personal Message Send Message Share on Social Media Radiation From Cardiac Imaging Tests Andrew J. Einstein Circulation. 2013; 127: e495-e497 , ...

  10. 76 FR 30109 - Marine Mammals; File No. 15453

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... Aquarium, 2777 Kalakaua Avenue Honolulu, HI 96815 (Dr. Andrew Rossiter, Responsible Party), has requested a..., HI 96814-4700; phone (808)944-2200; fax (808)973-2941. Written comments on this application should...

  11. Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, et al, 2010 American Geophysical Union Poster Session

    DOE Data Explorer

    Ezra Zemach

    2010-01-01

    Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, Brigette Martini, Chet Lide, Darrick Boschmann, John DIlles, Andrew Meigs, 2010 Ormat Nevada, Zonge Geophysical, Oregon State University American Geophysical Union, Poster Session

  12. 78 FR 63459 - Notice of Active Duty Determination

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... Affairs. FOR FURTHER INFORMATION CONTACT: Mr. Bruce T. Brown, Executive Secretary, DoD Civilian/Military Service Review Board, 1500 West Perimeter Road, Suite 3700, Joint Base Andrews, NAF Washington, MD...

  13. 77 FR 61755 - Performance Review Board Members

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ... LESLIE LEWIS TERESA LU MICHAEL METTLER ERIK MILLER TERESA MUNTZ DAVID MURPHY JUDITH NOVY STEVEN OLIN... TIMOTHY WILSON LEE ANDREW WOESTELL MEGAN WRIGHT DAVID YOUNG JASON Non-SES Employees Employee last...

  14. When Do Plumped-Up Lips Become 'Too Much'?

    MedlinePlus

    ... quite obvious," said Dr. Andrew Salzberg, chief of plastic surgery for the Mount Sinai Health System. Now, ... researcher Dr. Sang Kim. He is a facial plastic surgeon with Natural Face Clinics in Syracuse, N. ...

  15. Detail of parachute tower showing integration with main roof form, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of parachute tower showing integration with main roof form, facing southwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  16. 77 FR 71575 - Initiation of Antidumping and Countervailing Duty Administrative Reviews and Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-03

    ... Shoes Plant Shaoxing Andrew Metal Manufactured Shaoxing Dingli Metal Clotheshorse Co., Ltd. Shaoxing... in effect for company/government officials as well as their representatives in all segments of...

  17. The African Women's Protocol: bringing attention to reproductive rights and the MDGs.

    PubMed

    Gerntholtz, Liesl; Gibbs, Andrew; Willan, Samantha

    2011-04-01

    Andrew Gibbs and colleagues discuss the African Women's Protocol, a framework for ensuring reproductive rights are supported throughout the continent and for supporting interventions to improve women's reproductive health, including the MDGs.

  18. Needed: An 'Action Plan' for Kids Prone to Severe Allergic Reactions

    MedlinePlus

    ... safe and very effective," said Dr. J. Andrew Bird, a pediatric allergist at University of Texas Southwestern ... of using it, than not using it," said Bird, who wasn't involved in the AAP reports. ...

  19. UNDERSTANDING PATHWAYS OF TOXICITY: MAKING SENSE OF CHANGING SIGNALS

    EPA Science Inventory

    Title:
    Understanding Pathways of Toxicity: Making sense of changing signals
    Authors & affiliations:
    Sid Hunter, Maria Blanton, Edward Karoly, Ellen Rogers, Leonard Mole, Phillip Hartig, James Andrews. Reproductive Toxicology Division, National Health and Environmental Ef...

  20. DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE

    EPA Science Inventory

    DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE. JE Andrews, H Nichols, and ES Hunter. Reproductive Toxicology Division, NHEERL, USEPA, RTP, NC.

    Disinfection of drinking water with chlorine results in numerous chlorinated byprodu...

  1. Computers, Electronic Networking and Education: Some American Experiences.

    ERIC Educational Resources Information Center

    McConnell, David

    1991-01-01

    Describes new developments in distributed educational computing at Massachusetts Institute of Technology (MIT, "Athena"), Carnegie Mellon University ("Andrew"), Brown University "Intermedia"), Electronic University Network (California), Western Behavioral Sciences Institute (California), and University of California,…

  2. 77 FR 27109 - Certification Related to the Khmer Rouge Tribunal; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-08

    ... of the Independent Counselor and the effect it has had on the perception of the court--that the ECCC... international co-prosecutor, Andrew Cayley, dissatisfied with the amount and depth of evidence, requested...

  3. 47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. W. ANDREWS PATENT PATTERN DEVICE, C. 1870, VIEW LOOKING FROM THE FRONT, SECOND FLOOR SOUTH. - Watkins Mill, County Highway MM, Lawson, Ray County, MO

  4. 10. VIEW NORTH FROM FLOOR LEVEL OF DRYDOCK OF TOW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. VIEW NORTH FROM FLOOR LEVEL OF DRY-DOCK OF TOW TANK No. 1, WITH TORPEDO BODY BEING PREPARED FOR TANK TESTING. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  5. 78 FR 64535 - Notice of Appointment of Individuals To Serve as Members of Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ... Member--Catherine DeFilippo Member-- William E. Dobryzkowski Member --Robert B. Koopman Member--Karen Laney Member--Andrew Martin Member--Margaret D. MacDonald Member--Stephen A. McLaughlin Member--Lyn...

  6. 78 FR 13706 - Notice of Appointment of Individuals To Serve as Members of Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Dean A. Pinkert Member--Catherine DeFilippo Member--Robert B. Koopman Member--Karen Laney Member--Bill Dobryzkowski Member--Andrew Martin Member--Stephen A. McLaughlin Member--Lyn M Schlitt FOR FURTHER...

  7. 75 FR 70289 - Notice of Appointment of Individuals to Serve as Members of Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ...Filippo Member: Robert B. Koopman Member: Karen Laney Member: Lynn I. Levine Member: James M. Lyons Member: Stephen A. McLaughlin Member: Lyn M. Schlitt Member: Andrew Martin This notice is published in the...

  8. Autumn MIST 2009

    NASA Astrophysics Data System (ADS)

    Forsyth, Colin

    2010-02-01

    MEETING REPORT Colin Forsyth, Nicola Longden, Andrew Walsh and Robert Wicks summarize a MIST meeting where ground-based ionospheric science came under the spotlight, amid the broader concerns of the MIST community.

  9. 75 FR 60861 - Qualification of Drivers; Exemption Renewals; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    ..., Edwin A. Betz, Donald L. Carman, Mitchell L Carman, Christopher R. Cone, Walter O. Connelly, Stephen B.... Jamison, James A. Jones, Ronnie M. Jones, Andrew C. Kelly, Jason W. King, James T. Leek, Billy J....

  10. 75 FR 44050 - Qualification of Drivers; Exemption Applications; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ..., Walter O. Connelly, Stephen B. Copeland, Armando P. D'Angeli, Donald R. Davis, Ivory Davis, Louis A. Di..., Charles S. Huffman, Jesse P. Jamison, James A. Jones, Ronnie M. Jones, Andrew C. Kelly, Jason W....

  11. A Coherent VLSI Design Environment.

    DTIC Science & Technology

    2014-09-26

    Circuit Grammar for Operational Amplifier Design.’ PhD Thesis, Department of Electrical Engineering and Computer Science. MIT. Janu- ary, 1984; also MIT...Andrew L. Ressler, "A Circuit Grammar for Operational Amplifier Design." PhD Thesis, Department of Electrical Engineering and Computer Science. MIT...ISA(7HL E tT T O!I VLSI Memo No. 84-211 November 1984 A Circuit Grammar for Operational Amplifier Design* *! Andrew Lewis Ressler** Electrical circuit

  12. Advanced Kinetic-Based Modeling Applied to Plasma and Neutral Flows

    DTIC Science & Technology

    2012-09-01

    Advanced Kinetic-Based Modeling Applied to Plasma and Neutral Flows Briefers: Andrew Ketsdever Sergey Gimelshein PIs: Andrew Ketsdever...number. 1. REPORT DATE SEP 2012 2. REPORT TYPE 3. DATES COVERED 00-00-2012 to 00-00-2012 4. TITLE AND SUBTITLE Advanced Kinetic-Based Modeling ...magnetic field in opposite direction of applied field Extreme pressure tends to drive plasma out of discharge chamber Difficulties in modeling FRCs High

  13. Silicon-Containing Tri- and Tetra-Functional Cyanate Esters: Synthesis, Cure Kinetics, and Network Properties

    DTIC Science & Technology

    2014-01-01

    Correspondence to: Andrew J. Guenthner (E-mail: andrew.guenthner@edwards.af.mil) Additional Supporting Information may be found in the online version...performance. Because the carbon-silicon bond is in general longer and more flexible than a corresponding carbon-   3    carbon bond, the molecular segments... corresponding networks formed from di-functional cyanate esters without silicon. We further show that these advantages are retained when the

  14. Obtaining Cue Rate Estimates for Some Mysticete Species using Existing Data

    DTIC Science & Technology

    2014-09-30

    Roth at the Naval Postgraduate School in Monterey. OBJECTIVES An emerging area of study in the field of marine mammal passive acoustics is the...DCLDE Workshop, St. Andrews, Scotland , 12 June. D’Spain, G. L., T. A. Helble, H. H. Batchelor, and D. Rimington (2014). “Statistical mechanics...Campbell, and K. D. Heaney (2013c). “Calibrating call counts from single fixed sensors,” DCLDE Workshop, St. Andrews, Scotland , 14 June. Helble, T. A

  15. Credible Set Estimation, Analysis, and Applications in Synthetic Aperture Radar Canonical Feature Extraction

    DTIC Science & Technology

    2015-03-26

    CREDIBLE SET ESTIMATION, ANALYSIS, AND APPLICATIONS IN SYNTHETIC APERTURE RADAR CANONICAL FEATURE EXTRACTION THESIS Andrew C. Rexford, 1st Lieutenant...AND APPLICATIONS IN SYNTHETIC APERTURE RADAR CANONICAL FEATURE EXTRACTION THESIS Presented to the Faculty Department of Electrical and Computer...APPLICATIONS IN SYNTHETIC APERTURE RADAR CANONICAL FEATURE EXTRACTION THESIS Andrew C. Rexford, B.S.E.E. 1st Lieutenant, USAF Committee Membership: Dr. Julie

  16. Counterinsurgency: Clear-Hold-Build and the Pashtun Tribes in Afghanistan

    DTIC Science & Technology

    2012-06-08

    Taliban. The roads allowed access to more remote areas. A statement by General Karl Eikenberry left no doubt about the idea of roads being critical...counterinsurgency_field_manual_afghanistan_edition?page=0,1 (accessed 13 March 2012). 83Andrew Wilder, “Winning Hearts and Minds?” http://afghanistanforum.wordpress.com/tag/ karl -eikenberry...Wilder, Andrew. “Winning Hearts and Minds?” http://afghanistanforum.wordpress.com/tag/ karl - eikenberry/ (accessed 13 March 2012). World Food

  17. Efficient Sensitivity Methods for Probabilistic Lifing and Engine Prognostics

    DTIC Science & Technology

    2010-09-01

    Garza, D. Wagner, Andrew Bates, and Andy Voorhees University of Texas at San Antonio SEPTEMBER 2010 Final Report Approved...5c. PROGRAM ELEMENT NUMBER 62102F 6. AUTHOR(S) Harry Millwater, Ronald Bagley, Jose Garza, D. Wagner, Andrew Bates, and Andy Voorhees 5d...Development, Volume 1 – POD Analysis, AFRL-ML-WP-TR-2001- 4010, Jan. 2000. 7. Miedlar P, Berens A, Hovey P, Boehnlein T, Loomis J, “PRoF v3 PRobability Of

  18. Post-Vitrification Cure Kinetics of High Temperature Composite Resins: Implications for Characterization and Performance

    DTIC Science & Technology

    2013-05-01

    Guenthner , Josiah Reams, Christopher Sahagun, Matthew Davis, Joseph Mabry 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER Q0BG 7...PERFORMANCE Andrew J. Guenthner , 1 Josiah T. Reams, 2 Christopher M. Sahagun, 3 Matthew C. Davis, 4 and Joseph M. Mabry 1 1 Aerospace Systems...6301. 15 5. Reams, Josiah. T., Guenthner , Andrew J., Lamison, Kevin R., Vij, Vandana, Lubin, Lisa M., and Mabry, Joseph M. “Effect of Chemical

  19. Directed Self-Assembly and Morphology of Aryl-Substituted POSS in Polyetherimide Films

    DTIC Science & Technology

    2009-10-22

    Substituted POSS in Polyetherimide Films 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Andrew J. Guenthner , Gregory R. Yandek...Assembly and Morphology of Aryl-Substituted POSS in Polyetherimide Films Andrew J. Guenthner ,1 Gregory R. Yandek,1 Kevin R Lamison,2 and Joseph M...Fangchiang, M.-H. J. Poly. Sci., Prt. A: Polym. Chem. 2008, 46(15), 5157-5166. 6. Tomczak, S. J.; Wright, M. E.; Guenthner , A. J.; Petteys, B. J

  20. High-Performance Bio-Based Cyanate Esters with Low Moisture Uptake

    DTIC Science & Technology

    2012-04-01

    Uptake 5a. CONTRACT NUMBER In-House 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER Andrew Guenthner , Matthew...release, distribution is unlimited. HIGH-PERFORMANCE BIO-BASED CYANATE ESTER RESINS WITH LOW MOISTURE UPTAKE Andrew J. Guenthner ,1 Matthew C. Davis,2...275. 2. Illicium, pimpinella, and foeniculum. Jodral, M. M., Ed.; CRC Press: Washington, DC, 2004. 3. Guenthner , A. J.; Lamison, K. R.; Vij, V

  1. The Effect of Atomic Oxygen on POSS-Polyimides (Preprint)

    DTIC Science & Technology

    2008-05-01

    NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) S. Tomczak & J. Mabry (AFRL/RZSM); M. Wright, B. Petteys, & A. Guenthner (NAVAIR- 5d. PROJECT...Sandra J.; Vij, Vandana; Minton, Timothy K.; Brunsvold, Amy L.; Marchant, Darrell; Wright, Michael E.; Petteys, Brian J.; Guenthner , Andrew J.; Yandek...Wright, Michael E.; Petteys, Brian J.; Guenthner , Andrew J.; Svejda, Steven A.; Mabry, Joseph M., “Synthesis and Characterization of Space

  2. Effects of Thin High-z Layers on the Hydrodynamics of Laser-Accelerated Plastic Targets

    DTIC Science & Technology

    2002-01-01

    support. We acknowledge very helpful discussions with John Sethian, Andrew Mostovych and Stephen Bodner. We thank Tom Walsh and Ed Hsiegh, Schafer...Kalantar, M.H. Key, B. A. Remington, J. E. Rothenberg, E. Wulfrum, C. P. Verdon , and J. P. Knauer, Phys. Plasmas 4, 1978 (1997). 4 Andrew J. Schmitt, A...823 (1984). 10 Nathan Metzler, Alexander Velikovitch, and John H, Gardner, Phys. Plasmas 8, (1999) 11 M. Desselberger, T. Afshar-rad, F. Khattak, S

  3. Experimental Generation of non-Kolmogorov Turbulence using a Liquid Crystal Spatial Light Modulator

    DTIC Science & Technology

    2011-01-01

    performance evaluations. ACKNOWLEDGEMENTS This research was performed while the author Italo Toselli holds a National Research Council Research...REFERENCES 1. Larry C. Andrews, Ronald L. Phillips. Laser Beam Propagation through Random Media, 2nd ed. (SPIE, 2005). 2. Italo Toselli , Larry C...2008). 3. Italo Toselli , Larry C. Andrews, Ronald L. Phillips, Valter Ferrero. Free space optical system performance for a Gaussian beam propagating

  4. DECAF - Density Estimation for Cetaceans from Passive Acoustic Fixed Sensors

    DTIC Science & Technology

    2010-01-01

    DECAF – Density Estimation for Cetaceans from passive Acoustic Fixed sensors Len Thomas CREEM, University of St Andrews, St Andrews, Fife, Scotland...REPORT DATE 2010 2. REPORT TYPE 3. DATES COVERED 00-00-2010 to 00-00-2010 4. TITLE AND SUBTITLE DECAF - Density Estimation for Cetaceans from...Prescribed by ANSI Std Z39-18 LONG-TERM GOALS Determining the spatial density and distribution of cetacean (whale and dolphin) species is fundamental to

  5. Research in Mathematics and Computer Science: Calculation of the Probability of Undetected Error for Certain Error Detection Codes. Phase 1

    DTIC Science & Technology

    1989-03-31

    pectively. Figure 3. Code Weight Viewor for Dual of Code C2. -9- IQUALCOMM. Inc. Final Technical Repo for the Research in Mafthmatics and Cmputer Sience ...efforts. ANDREW J. VITERBI VICE CHAIRMAN AND CHIEF TECHNICAL OFFICER Education : Andrew J. Viterbi received the S.B. and S.M. degrees in Electrical...Coding, (with J.K. Omura), McGraw-Hill, 1979. JACK K. WOLF ENGINEERING ASSOCIATE Education : Jack Keil Wolf is a chaired professor in the Center for

  6. Using EM to Classify Text from Labeled and Unlabeled Documents

    DTIC Science & Technology

    2007-11-02

    prolog sec postscript knowledge postscript solution human exam quiz representation solution chapter field assaf ascii Table 2: Lists of the words...examples have used active learning, in which an algorithm iteratively selects an unlabeled example, asks a human labeler for its classification, and...Andrew McCallum, Ronald Rosenfeld. Tom Mitchell, and Andrew Ng. Improving text clasification by shrinkage in a hierarchy of classes. In 1CML-9S, 1998

  7. Adaption of the Air Weather Service Fog Model to Forecast Radiation Fog Events in the Southeast United States

    DTIC Science & Technology

    1997-03-01

    ADAPTATION OF THE AIR WEATHER SERVICE FO MODEL TO FORECAST RADIATION FOG EVENT IN THE SOUTHEAST UNITED STATES THESIS Andrew C. Goodnite, Captain...ENP/97M-06 ADAPTATION OF THE AIR WEATHER SERVICE FOG MODEL TO FORECAST RADIATION FOG EVENTS IN THE SOUTHEAST UNITED STATES THESIS Andrew C. Goodnite...AIR WEATHER SERVICE FOG MODEL TO FORECAST RADIATION FOG EVENTS IN THE SOUTHEAST UNITED STATES THESIS Presented to the Faculty of the Graduate School of

  8. Summary of the Proceedings of the National Symposium on Fracture Mechanics (17th) Held on 7-9 Aug 84 in Albany, New York.

    DTIC Science & Technology

    1986-12-01

    THE SEVENTEENTH NATIONAL SYMPOSIUM ON FRACTURE MECHANICS 00 rN JOHN H. UNDERWOOD DAVID P. WILHEM RICHARD CHAIT WAYNE R. ANDREWS 00 C. WILLIAM SMITH...AUTHOR.s) S. CONTRACT OR GRANT NUMBER(.) John H. Underwood, Richard Chait, C. William Smith, David P. Wilhem , Wayne R. Andrews, and James C. Newman (See...AUTHORS (CONT’D) John H. Underwood U.S. Army Armament Research, Development, and Engineering Center Benet Weapons Laboratory Watervli et, NY Richard

  9. Behavioral Responses of Naive Cuvier’s Beaked Whales in the Ligurian Sea to Playback of Anthropogenic and Natural Sounds

    DTIC Science & Technology

    2014-09-30

    University of St Andrews,Scottish Oceans Institute,Sea Mammal Research Unit,St Andrews, KY16 8LB United Kingdom , 8. PERFORMING ORGANIZATION REPORT...7m hand-held carbon fiber pole. Tracking and data collection - To visually search for animals in the search phase, and to observe the behavior of...the animals during tagging and tracking, a marine mammal observer platform was installed on the deck of the Aleph. Observers scanned with naked eye

  10. Development of a Team Scenario Content Generation Framework

    DTIC Science & Technology

    2010-03-01

    Development of a Team Scenario Content Generation Framework Andrew Morton Barbara D. Adams Project Manager: Barbara D. Adams Contract No. W7711... GENERATION FRAMEWORK by: Andrew Morton and Barbara D. Adams Humansystems® Incorporated 111 Farquhar Street Guelph, ON N1H 3N4 Project... Generation Framework Page i Abstract Web-based software called ELICIT (Experimental Laboratory for the Investigation of Collaboration, Information-sharing

  11. Investigation of Antiangiogenic Mechanisms Using Novel Imaging Techniques

    DTIC Science & Technology

    2010-02-01

    Biomed. Opt. 95, 982–994 2004. 6. M. Khurana, E. H. Moriyama, A. Mariampillai, and B. C. Wilson, “ Intravital high-resolution optical imaging of...Mechanisms Using Novel Imaging Techniques PRINCIPAL INVESTIGATOR: Andrew Fontanella CONTRACTING ORGANIZATION: Duke University Durham...Using Novel Imaging Techniques 5b. GRANT NUMBER W81XWH-09-1-0113 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER Andrew Fontanella

  12. US Army Europe Integrated Training Area Management (ITAM) Program - Training Support and the Environment

    DTIC Science & Technology

    2010-06-01

    1UNCLASSIFIED Supporting 7th ArmyReady Thru Training! Integrated Training Area Management Jeff Andrews USAREUR ITAM Operations Coordinator...Jeffrey.a.andrews@us.army.mil Army Sustainable Range Program (SRP) US Army Europe Integrated Training Area Management ( ITAM ) Program – Training Support and...Management ( ITAM ) Program - Training Support and the Environment 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d

  13. US Army Europe: Sharing the ITAM Tenets with Multinational Partners in Africa

    DTIC Science & Technology

    2011-05-01

    Any Mission, Anywhere UNCLASSIFIED As of 5 May 2011 USAREUR; Sharing ITAM TenetsUNCLASSIFIED Jeff Andrews USAREUR ITAM Operations Coordinator...Jeffrey.a.andrews@us.army.mil Army Sustainable Range Program (SRP) US Army Europe: Sharing the ITAM Tenets with Multinational Partners in Africa Report...REPORT TYPE 3. DATES COVERED 00-00-2011 to 00-00-2011 4. TITLE AND SUBTITLE US Army Europe: Sharing the ITAM Tenets with Multinational

  14. Sustainability vs. Enhancement: What is the Future Priority of Land Rehabilitation and Maintenance (LRAM)?

    DTIC Science & Technology

    2011-05-01

    and Maintenance (LRAM)? Jeffrey Andrews US Army Europe ITAM Operations Manager jeffrey.a.andrews@us.army.mil Report Documentation Page Form...Prescribed by ANSI Std Z39-18 Any Mission, Anywhere UNCLASSIFIED As of 29 Apr 2011 UNCLASSIFIED  ITAM is one of two DA G3 “Enabler” programs (RTLP...and ITAM ) that supports the objectives of the Army Sustainable Range Program (SRP) with the goal of maximizing the capability, availability, and

  15. Seeded Fault Bearing Experiments: Methodology and Data Acquisition

    DTIC Science & Technology

    2011-06-01

    Seeded Fault Bearing Experiments: Methodology and Data Acquisition by Andrew J. Bayba, Derwin Washington, and Kwok Tom ARL-TR-5575 June...TR-5575 June 2011 Seeded Fault Bearing Experiments: Methodology and Data Acquisition Andrew J. Bayba, Derwin Washington, and Kwok Tom...TYPE Final 3. DATES COVERED (From - To) October 2010 to March 2011 4. TITLE AND SUBTITLE Seeded Fault Bearing Experiments: Methodology and Data

  16. The Characterization Of The Kinetics Of Chlamydia Muridarum Infection In Defined Regions Of The Murine Genital Tract

    DTIC Science & Technology

    2008-08-01

    R. R. Ingalls, C. W. Andrews, Jr., A. M. Scurlock, and T. Darville . 2007. Plasmid-deficient Chlamydia muridarum fail to induce immune pathology...and protect against oviduct disease. J. Immunol. 179:4027-4034. 49 8. Darville , T., C. W. Andrews, Jr., and R. G. Rank. 2000. Does inhibition of...Sufficient IFN-gamma to Mediate Robust Protective Immunity against Genital Chlamydia muridarum Infection. J. Immunol. 180:3375-3382. 19. Darville , T

  17. Insights into Surface Structure and Performance of Fluorinated Silicates from Cohesive Energy Studies

    DTIC Science & Technology

    2016-03-17

    fluorinated silicates from cohesive energy studies 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Andrew J...Insights into surface structure and performance of fluorinated silicates from cohesive energy studies 17 March 2016 Andrew J. Guenthner,1 Timothy...distribution is unlimited. PA Clearance Number 16153 Comparison of Surface Energy Parameters for Fluorosilicates 5 • Typical Surface Energies : -CF3 = 6

  18. Report of Freshwater Mussels Workshop Held at St. Louis, Missouri on 26-27 October 1982.

    DTIC Science & Technology

    1983-10-01

    CHART NATIOfAL B4JREMJ Of STANDARDS- 1963-A %, V C3 C II -~ REPORT OF FRESHWATER MUSSELS WORKSHOP 26-27 October 19820 Andrew C. Miller, Compiler U. S ...Distribution Unlimited AA .. q, Sponsored by Office, Chief of Engineers, U. S . Army Washington, D. C. 20314 Published by U.S. Army Engineer Waterways...Field trip on the Meramec River, Missouri. Times Beach Access, on 27 October 1982. Photograph by Andrew Miller. U. S . Army Engineer Waterways

  19. An Analysis of Freshwater Mussels (Unionidae) in the Lower Ohio River at Two Beds Near Olmsted, Illinois: 1992 Studies.

    DTIC Science & Technology

    1994-02-01

    of Freshwater Mussels (Unionidae) in the Lower Ohio River at Two Beds Near Olmsted, Illinois: 1992 Studies by Barry S . Payne, Andrew C. Miller...1992 Studies by Barry S . Payne, Andrew C. Miller, Deborah Shafer Environmental Laboratory U.S. Army Corps of Engineers Accesion For Waterways...Station Cataloging-in-Publication Data Payne, Barry S . An analysis of freshwater mussels (Unionidae) in the Lower Ohio River at two beds near Olmsted

  20. Germany, the European Union, and the Euro: The Primacy of Politics in Treasure

    DTIC Science & Technology

    2013-09-01

    12 Andrew C. Sobel , International Political Economy in Context: Individual Choices, Global Effects (Washington, DC: CQ Press...Wadbrook, West German Balance-of-Payments Policy, 54–62. 92 Lynn, Bust, 78. 93 Sobel , International Political Economy in Context, 388. 94 Lynn, Bust...Between Hitler and Stalin. New York: Basic Books, 2010. Sobel , Andrew C. International Political Economy in Context: Individual Choices, Global

  1. Relocation of Joint Munitions Storage Area. Environmental Assessment

    DTIC Science & Technology

    2008-10-01

    Surface Water Surface waters are located in three significantly diverse watersheds (Potomac, Anacostia , and Patuxent rivers ) that drain 2,317 square...of Prince George’s County, and 132,000 acres drain to the Anacostia River (west of Andrews AFB). The majority of Andrews AFB is located in the...would be prepared by the contractor performing the work. At a minimum, the contractor would prepare a MDE Erosion and Sediment Control Plan and MDE

  2. Contactless Microwave Measurements of Photoconductivity in Silicon Hyperdoped with Chalcogens

    DTIC Science & Technology

    2012-01-01

    photocarrier lifetime, hyperdoped silicon David Hutchinson, Thomas Cruson, Anthony DiFranzo, Andrew McAllister, Aurore J. Said, Jeffrey M. Warrender, Daniel...Chalcogens Daniel Recht, David Hutchinson1, Thomas Cruson1, Anthony DiFranzo1, Andrew McAllister1, Aurore J. Said, Jeffrey M. Warrender2, Peter D. Persans1...microwaves emitted by a Millitech Gunn diode pass, via a waveguide , through an isolator to protect the source from reflections. A ‘‘magic tee’’ then

  3. Dynamic Response of an Insonified Sonar Window Interacting with a Tonpilz Transducer Array

    DTIC Science & Technology

    2007-01-03

    NUWC-NPT Technical Report 11,781 3 January 2007 Dynamic Response of an Insonified Sonar Window Interacting with a Tonpilz Transducer Array Andrew J...Code 1516) for their discussions on Tonpilz transducer behavior Reviewed and Approved: 3 January 2007 s S. Griffin Head, Autonomous Systems and...FUNDING NUMBERS Dynamic Response of an Insonified Sonar Window Interacting with a Tonpilz Transducer Array 6. AUTHOR(S) Andrew J. Hull 7. PERFORMING

  4. Unique ligation properties of eukaryotic NAD+-dependent DNA ligase from Melanoplus sanguinipes entomopoxvirus.

    PubMed

    Lu, Jing; Tong, Jie; Feng, Hong; Huang, Jianmin; Afonso, Claudio L; Rock, Dan L; Barany, Francis; Cao, Weiguo

    2004-09-01

    The eukaryotic Melanoplus sanguinipes entomopoxvirus (MsEPV) genome reveals a homologous sequence to eubacterial nicotinamide adenine dinucleotide (NAD(+))-dependent DNA ligases [J. Virol. 73 (1999) 533]. This 522-amino acid open reading frame (ORF) contains all conserved nucleotidyl transferase motifs but lacks the zinc finger motif and BRCT domain found in conventional eubacterial NAD(+) ligases. Nevertheless, cloned MsEPV ligase seals DNA nicks in a NAD(+)-dependent fashion, while adenosine 5'-monophosphate (ATP) cannot serve as an adenylation cofactor. The ligation activity of MsEPV ligase requires Mg(2+) or Mn(2+). MsEPV ligase seals sticky ends efficiently, but has little activity on 1-nucleotide gap or blunt-ended DNA substrates even in the presence of polyethylene glycol. In comparison, bacterial NAD(+)-dependent ligases seal blunt-ended DNA substrates in the presence of polyethylene glycol. MsEPV DNA ligase readily joins DNA nicks with mismatches at either side of the nick junction, except for mismatches at the nick junction containing an A base in the template strand (A/A, G/A, and C/A). MsEPV NAD(+)-dependent DNA ligase can join DNA probes on RNA templates, a unique property that distinguishes this enzyme from other conventional bacterial NAD(+) DNA ligases. T4 ATP-dependent DNA ligase shows no detectable mismatch ligation at the 3' side of the nick but substantial 5' T/G mismatch ligation on an RNA template. In contrast, MsEPV ligase joins mismatches at the 3' side of the nick more frequently than at the 5' side of the nick on an RNA template. The complementary specificities of these two enzymes suggest alternative primer design for genomic profiling approaches that use allele-specific detection directly from RNA transcripts.

  5. Biochemical characterization of the DNA ligase I from Entamoeba histolytica.

    PubMed

    Cardona-Felix, Cesar S; Pastor-Palacios, Guillermo; Cardenas, Helios; Azuara-Liceaga, Elisa; Brieba, Luis G

    2010-11-01

    DNA ligases play an essential role in DNA replication and repair. Herein, we report the cloning and biochemical characterization of DNA ligase I from the protozoan parasite Entamoeba histolytica (EhDNAligI). EhDNAligI is an ATP-dependent DNA ligase of 685 amino acids with 35% identity to human DNA ligase I. This report shows that heterologous expressed EhDNAligI is able to perform the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. EhDNAligI is strongly inhibited by NaCl and displays optimal activity at pH 7.5. EhDNAligI uses Mn2+ or Mg2+ as metal cofactors and ATP as nucleotide cofactor. EhDNAligI has a nicked DNA binding constant of 6.6microM and follows Michaelis-Menten steady-state kinetics with a K(m) ATP of 64nM and a k(cat) of 2.4min(-1). Accordingly to its properties as a family I DNA ligase, EhDNAligI is able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position. We propose that EhDNAligI is involved in sealing DNA nicks during lagging strand synthesis and may have a role in base excision repair in E. histolytica.

  6. Expression, purification and biochemical characterization of Methanocaldococcus jannaschii DNA ligase.

    PubMed

    Wang, You; Xie, Juan-Juan; Han, Zhong; Liu, Jian-Hua; Liu, Xi-Peng

    2013-02-01

    We describe the biochemical characterization of Methanocaldococcus jannaschii (M. jannaschii) DNA ligase and its potential application in single nucleotide polymorphism (SNP) genotyping. The recombinant M. jannaschii DNA ligase is an ATP-dependent ligase. The ligase activity was dependent on metal ions of Mg(2+) and Mn(2+). The optimal concentrations of ATP cofactor and Mg(2+) ion were 0.01-2 and 10 mM, respectively. The optimal pH value for DNA ligation was 8.5. High concentrations of NaCl inhibited DNA ligation. The effects of mismatches on joining short oligonucleotides by M. jannaschii DNA ligase were fully characterized. The mismatches at the first position 5' to the nick inhibited ligation more than those at the first position 3' to the nick. The mismatches at other positions 5' to the nick (3rd to 7th sites) exhibited less inhibition on ligation. However, the introduction of a C/C mismatch at the third position 5' to the nick could completely inhibit the ligation of the terminal-mismatched nick of an oligonucleotide duplex by M. jannaschii DNA ligase. Therefore, introducing an additional mismatch at the third position 5' to the SNP site is a more effective approach in genotyping by M. jannaschii DNA ligase.

  7. Unusual reactivity in a commercial chromium supplement compared to baseline DNA cleavage with synthetic chromium complexes.

    PubMed

    Chaudhary, Shveta; Pinkston, Joel; Rabile, M Mohamed; Van Horn, J David

    2005-03-01

    Commercially available chromium supplements were tested for their DNA cleavage ability compared with synthetic chromium(III) complexes, including chromium(III) tris-picolinate [Cr(pic)3], basic chromium acetate [Cr3O(OAc)6]+, model complexes, and recently patented Cr-complexes for use in supplements or therapy. Four different supplements (P1-P4) were tested for their DNA cleaving activity in the presence and the absence of H2O2, dithiothreitol (DTT) or ascorbate. One supplement, P1, showed nicking of DNA in the absence of oxidant or reductant at 120 microM metal concentration. Different lot numbers of P1 were also tested for DNA cleavage activity with similar results. Commercial supplements containing Cr(pic)3 nicked DNA at 120 microM metal concentrations in the presence of 5 mM ascorbate or with excess hydrogen peroxide, analogous to reactions with synthetic Cr(pic)3 reported elsewhere. Another chromium (non-Cr(pic)3) supplement, P2, behaves in a comparable manner to simple Cr(III) salts in the DNA nicking assay. Chromium(III) malonate [Cr(mal)2] and chromium(III) acetate [Cr(OAc)] can nick DNA in the presence of ascorbate or hydrogen peroxide, respectively, only at higher metal concentrations. The Cr(III) complexes of histidine, succinate or N-acetyl-L-glutamate do not nick DNA to a significant degree.

  8. Maternal hurricane exposure and fetal distress risk.

    PubMed

    Zahran, Sammy; Snodgrass, Jeffrey G; Peek, Lori; Weiler, Stephan

    2010-10-01

    Logistic regression and spatial analytic techniques are used to model fetal distress risk as a function of maternal exposure to Hurricane Andrew. First, monthly time series compare the proportion of infants born distressed in hurricane affected and unaffected areas. Second, resident births are analyzed in Miami-Dade and Broward counties, before, during, and after Hurricane Andrew. Third, resident births are analyzed in all Florida locales with 100,000 or more persons, comparing exposed and unexposed gravid females. Fourth, resident births are analyzed along Hurricane Andrew's path from southern Florida to northeast Mississippi. Results show that fetal distress risk increases significantly with maternal exposure to Hurricane Andrew in second and third trimesters, adjusting for known risk factors. Distress risk also correlates with the destructive path of Hurricane Andrew, with higher incidences of fetal distress found in areas of highest exposure intensity. Hurricane exposed African-American mothers were more likely to birth distressed infants. The policy implications of in utero costs of natural disaster exposure are discussed.

  9. Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis

    PubMed Central

    Poole, Brian D.; Kivovich, Violetta; Gilbert, Leona; Naides, Stanley J.

    2011-01-01

    Parvovirus B19 is a widespread virus with diverse clinical presentations. The viral nonstructural protein, NS1, binds to and cleaves the viral genome, and induces apoptosis when transfected into nonpermissive cells, such as hepatocytes. We hypothesized that the cytotoxicity of NS1 in such cells results from chromosomal DNA damage caused by the DNA-nicking and DNA-attaching activities of NS1. Upon testing this hypothesis, we found that NS1 covalently binds to cellular DNA and is modified by PARP, an enzyme involved in repairing single-stranded DNA nicks. We furthermore discovered that the DNA nick repair pathway initiated by poly(ADPribose)polymerase and the DNA repair pathways initiated by ATM/ATR are necessary for efficient apoptosis resulting from NS1 expression. PMID:21278893

  10. Effects of DNA-binding drugs on T4 DNA ligase.

    PubMed Central

    Montecucco, A; Pedrali-Noy, G; Spadari, S; Lestingi, M; Ciarrocchi, G

    1990-01-01

    A number of DNA intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, AMP-dependent DNA topoisomerization and EDTA-induced DNA nicking mediated by bacteriophage T4 DNA ligase. The inhibition seems to arise from drug-substrate interaction so that formation of active DNA-Mg2(+)-AMP-enzyme complex is impaired while assembled and active complexes are not disturbed by drug binding to the substrate. Images Fig. 2. Fig. 4. Fig. 5. PMID:2156493

  11. Officers and Council, 1998-1999

    NASA Astrophysics Data System (ADS)

    1999-10-01

    The 1998-1999 Council of the British Astronomical Association photographed on the steps of Burlington House, London, on 1999 May 26. Front row, left to right: Bob Marriott, Hazel McGee, Martin Mobberley, Nick James, Jonathan Shanklin, Ron Johnson; centre: Richard Miles, Gordon Taylor, Jacqueline Mitton, David Tucker, John Mason, Pat Barber, David Reid, Peter Hudson; back: Laurence Anslow, Guy Hurst, Lionel Mayling, Nick Hewitt, Owen Brazell, Tony Kinder, Mark Armstrong, Maurice Gavin. Photo by Hazel McGee and Linda Newton.

  12. Natural Language Interfaces to Database Systems

    DTIC Science & Technology

    1988-10-01

    Towards a High Level User Interface,’ 4th International Conference E-R Approved (IEEE), Sept 1985, pp. 36-43. 173 Mimi Kao, Nick Cercone, WoShun Luk, Lab...147. 268 Leo Mark, Nick Roussopoulos, Dept. of Computer Science, University of Maryland, "Integration of Data, Schema and Meta-schema in the Context...Michael G. Dyer, Peter N. Johnson, CJ. Yang, Steve Harley , Dept. of Computer Science, Yale University, "BORIS - An Experiment in In-Depth Understanding of

  13. POW/MIA Issues. Volume 3. Appendixes

    DTIC Science & Technology

    1994-01-01

    Reom, Robert D. 2nd Lt 049636 A 5 55 Rowland, Eugene Z. Cpl 605440 A 5 56 Pardue, Billy J. Pfc 655275 A 5 57 Jack. Nick S. Pfc 854397 A 5 58 Bard, Ward 0...7 1 12 172 Cooke, Glen L. Cpl RA 19063036 1 12 173 Jones, William P. Sat RA 12285533 1 12 174 Krawcion(?), Nick 2bd Lt 0-2262262 1 12 175 Sutp...Stewart, Harley D. PFC 33403257 168. Stinson, Cornelius W. S SC 32608417 169. Stonry, Harold H. CPL 35171102 170. Tannler, Thomas K. 1 LT 0-026158 171

  14. Endocrine Therapy of Breast Cancer

    DTIC Science & Technology

    2009-06-01

    measured by terminal deoxynucleotidyl transferse-mediated dUTP nick -end labeling (TUNEL) staining (Wedam et al., 2006). In an in vitro model of lung...transformation of mammary cells. Carcinogenesis 2000; 21:871–879. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich...transferase dUTP nick end labeling (TUNEL) and cleavage of the DNA repair enzyme PARP-1 are associated with late apoptosis or necrosis [35]. 2.2

  15. Bibliography on Cold Regions Science and Technology. Volume 43. Part 2

    DTIC Science & Technology

    1989-12-01

    P.F.X. Thermosyphons and foundation design in cold regions, Harley , P.C. Comparison of freezing cells uscd in the determination of coil c1988, p.25...reliability by probability cost 43-2919 rion ea tan ert 9 developm ents inth3rserc 1o9cl methods (1987 , p.195-202, engl 43.1590 Model sce of the NICK lce...appends., eagj 43-720 Model ice of the NICK Ice Model Basin. NaritsS et al, CI1988, p.32-35, rutj 43-1078 Methods of preparing laboratory ice witls

  16. Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.

    PubMed

    Kikuchi, Y; Tyc, K; Filipowicz, W; Sänger, H L; Gross, H J

    1982-12-11

    A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2', 3'-cyclic phosphate and 5'-phosphate ends for ligation to form a 2'-phosphomonoester, 3',5'-phosphodiester bond. Using 5'-3 2P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms (formula; see text) U-bonds. Natural linear PSTV, however, forms mainly (formula; see text) A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2',3'-cyclophosphate termini.

  17. Pre-Conflict Peace Building as a Mission for USACE

    DTIC Science & Technology

    2009-04-29

    governance, education, health , trade and other development that are critical to peace building efforts in creating sustainable development. The successful...St ra te gy Re se ar ch Pr oj ec t PRE-CONFLICT PEACE BUILDING AS A MISSION FOR USACE BY MR. NICK M. PANASIUK Department of Army Civilian...Conflict Peace Building as a Mission for USACE 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Mr. Nick M. Panasiuk 5d. PROJECT

  18. Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

    SciTech Connect

    Yarosh, D.B.; Setlow, R.B.

    1981-03-01

    Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

  19. Human Histologic Repair and Regeneration After Biologic Preparation of Diseased Root Surfaces,

    DTIC Science & Technology

    1983-12-01

    six to eight cells thick, but with some rete extensions into the connective tissue , was attached to the root surface to a point about 0.7 mm coronal to... cell and the nick the connective tissue fibers and flattened fibroblasts were oriented parallel to the root surface (Fig. 5). 7. Patient "B". Control...lymphocytes subjacent to the epithelium. From the apical Junctional epithelial cell to the nick, a distance of about 0.5 as, there was connective tissue (Fig

  20. British Military Requirements, Resources, and Conventional Arms Control

    DTIC Science & Technology

    1990-01-01

    rates range between 2 and 2.5 percent. The growth rate in the 1See Sandier and Murdoch , 1986; and Don, 1986, for more detailed budget forecasting...Retreat, Brassey’s Defence Publishers, London, 1986. Cook, Nick , "W Germany, UK and Italy set to order more Tornados," Jane’s Defence Weekly, February 13...1988, p. 245. Cook, Nick , "UKs Bloodhound successor: BAe in MSAM studies," Jane’s Defence Weekly, March 26, 1988, p. 551. Coxhead, C.D., "Pressure

  1. Sequence stratigraphy and depositional systems of the paleocene submarine fans in the central North Sea: The evolution of a shelf-to-basin system

    SciTech Connect

    Reinsborough, B.C.; Galloway, W.E. )

    1993-09-01

    Slope/basin depositional systems consist of combinations of facies, including slump lobes; chute, flute, and channel fills; mounded turbidite lobes; sheet turbidites; low-density turbidite sheets and fills; hemipelagic drapes; and contourite mounds. Specific facies associations are determined by the nature (point source or linear source) and caliber (volume, grain size, sand:mud) of sediment supply to the slope. The extensive well-log, seismic, and core database was used to dissect the stratal and facies architecture of the Andrew depositional system and characterize a logical evolution of the sand-rich shelf-to-basin depositional systems tract. The andrew consists of upper and lower depositional units bounded by downlap terminations and high-gamma marker beds. The lower Andrew displays three distinct sand-rich lobes, delineated by isopach, sand percent, log motif, and seismic facies maps Proximal, mounded, sand-rich units disperse into unchannelized sheet turbidites in the basin. No extensive incised submarine valleys feed this unit, which is characterized by coarsening and thickening-upward log responses and hummocky to discontinuous reflectors. The upper Andrew downlaps the lower unit and a single, linear sediments source was centered in the Witch ground graben. The dispersal pattern and internal character suggest the upper unit is a proximal slope apron, downlapping and filling interlobe bathymetric lows of the underlying unit. Sharp-based, blocky/digitate log signatures, discontinuous chaotic reflectors, and coarse-grained sediment characterize this unit. The lower Andrew represents a structurally focused, sand-rich lobe complex, without associated incised canyons. The Andrew system evolved as the delta platform expanded onto the proximal fan, resulting in a linear sediment source spilling over the slope as a fringing slope apron.

  2. Triclinic Polymorph of Bis(triphenylsilyl) Oxide Toluene Disolvate

    DTIC Science & Technology

    2012-01-01

    Triclinic polymorph of bis(triphenylsilyl) oxide toluene disolvate Andrew P. Purdy,a* Emily Smoot,a‡ Ray J. Butcherb and Andrew Kerrc aNaval Research... triclinic (P1) instead of possessing the previously reported rhombohedral symmetry [Hönle et al. (1990). Acta Cryst. C46, 1982–1984]. Each of the –SiPh3... structures of related compounds, see: Glidewell & Liles (1978); Morosin & Harrah (1981); Suwińska et al. (1986). For the determination by IR

  3. Magnetic Field-Induced Phase Transformation in Magnetic Shape Memory Alloys with High Actuation Stress and Work Output

    DTIC Science & Technology

    2010-05-03

    Ferromagnetic Shape Memory Alloy Single Crystals,” Journal of Materials Science, Vol. 43, pp. 6890-6901, 2008. 10. Karaca H.E., Karaman I., Brewer A...Krishnendu Haldar 1.00 Ruixian Zhu 0.00 Cengiz Yegin 0.00 Sadegh Badakhshan Raz 1.00 Andrew L. Brewer 1.00 6.00FTE Equivalent: 8Total Number: Names...degrees NAME Ruixian Zhu Andrew L. Brewer 2Total Number: Names of personnel receiving PHDs NAME Bjoern Kiefer Haluk Ersin Karaca Burak Basaran 3Total

  4. Multi-species Cetacean Satellite Tagging to Examine Movements in Relation to the 2008 Rim-of-the-Pacific (RIMPAC) Naval Exercise

    DTIC Science & Technology

    2008-10-19

    Baird1, Gregory S. Schorr1, Daniel L. Webster2, Daniel J . McSweeney3, M. Bradley Hanson4, and Russel D. Andrews5 1Cascadia Research Collective...Antarctica. Polar Biology DOI 10.1007/s00300-008-0487-z. Baird, R.W., G.S. Schorr, R.D. Andrews, M.B. Hanson, D.L. Webster, D.J. McSweeney, and J ...Scientific Review Group, National Marine Fisheries Service, Monterey, California. Baird, R.W., D.L. Webster, G.S. Schorr, D.J. McSweeney and J . Barlow

  5. Insourcing and Outsourcing for U.S. Department of Defense IT Projects: A Model

    DTIC Science & Technology

    2011-01-01

    to divide IT into three discrete areas to assist in an organization’s decision making process of whether to in-source or outsource. Andrew McAfee...Contractor Cuts.” Federal Computer Week. (2009): 1 Loomis , Bill. “The Administration’s Push to Do More Work In-House Will Alter the Status Quo.” Washington...Technology. (2009): 1. McAfee, Andrew . “The Three Worlds of Information Technology.” Harvard Business Review. R0611J (2006): 1-9. McFarlane, Tom

  6. Operational Alignment in Predator Training Research

    DTIC Science & Technology

    2014-04-21

    RESEARCH Noah P. Schill*, Leah J. Rowe†, Brian L. Gyovai‡, DeForest Q. Joralmon§, Andrew J. Schneck**, Darrin A. Woudstra†† The sixteen year old USAF...Performance Wing, Warfighter Readiness Research Division. ‡ Lt Col Brian L. Gyovai, OHANG, 178th Operations Support Squadron. § Dr. DeForest Q...Noah P. Schill, Leah J. Rowe, Brian L. Gyovai, DeForest Q. Joralmon, Andrew J. Schneck, Darrin A. Woudstra 5d. PROJECT NUMBER 5e. TASK NUMBER 5f

  7. A Randomized Effectiveness Trial of a Systems Level Approach to Stepped Care for War Related PTSD

    DTIC Science & Technology

    2015-09-01

    Investi- gators: Fort Bliss—Melissa Molina, MD, Stanley Harmon, RN, MSN, FNP ; Fort Bragg—Lillie Walker, MPAS, PA-C, Andrew Fletcher, MD, LTC, MC, Thurman...Saunders, MSN, FNP -C, MAJ, AN; Fort Campbell—Lisa Ford, LTC, AN, FNP -BC, Vlatka Plymale, DO, CPT, MC, Jason Dailey, MD, MAJ, MC; Fort Carson—Nicholas...Harmon, RN, MSN, FNP ; Fort Bragg - Lillie Walker, MPAS, PA-C, Andrew Fletcher, MD, LTC, MC, Thurman Saunders, MSN, FNP -C, MAJ, AN; Fort Campbell - Lisa

  8. Packing Fraction and Relation to Glass Transition in Ternary Blends of Cyanate Ester Resins (PREPRINT)

    DTIC Science & Technology

    2010-10-21

    Blends of Cyanate Ester Resins 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Andrew J. Guenthner , Joseph M. Mabry (AFRL/RZSM...Resins Kevin R. Lamison’, Andrew J. Guenthner , Vandana Vij’, and Joseph M. Mabrl. ’ERG Incorporated, Edwards AFB, CA 93524 2Propu/sion Directorate...in Proc. SPE Conf. High Temp. Po/ym. Society of Plastics Engineers: Cleveland, OH, 1989; pp. 127-140. 6. Guenthner . A. J ,; Yandek, G. R.; Wright, M

  9. Evaluation of capacities of template-type Gulf of Mexico platforms

    SciTech Connect

    Bea, R.G.; Loch, K.J.; Young, P.L.

    1995-12-31

    This paper details results from nonlinear analyses of the ultimate limit state performance characteristics of four Gulf of Mexico (GOM) platforms subjected to intense loadings from hurricane Andrew. These four platforms were located to the east of the track of hurricane Andrew, and were thus in the most intense portion of the storm. The nonlinear analyses are able to replicate details of the observed behavior of the four structures. This replication is very dependent on realistic characterization of the performance characteristics of the pile foundations and an accurate information on the ``as is`` condition of the platforms before the storm.

  10. Predicting Carbonate Species Ionic Conductivity in Alkaline Anion Exchange Membranes

    DTIC Science & Technology

    2012-06-01

    anion exchange membranes. Andrew M. Kiss, Timothy D . Myles, Kyle N. Grew, Aldo A. Peracchio, George J. Nelson, and Wilson K. S. Chiu University of...Alkaline Anion Exchange Membranes Andrew M. Kiss1, Timothy D . Myles1, Kyle N. Grew2, Aldo A. Peracchio1, George J. Nelson1, and Wilson K. S. Chiu1* 1...Vol. 301, 93-106, 2007. 16. Satterfleld,M.B., Benziger,J.B., J. Phys. Chem. B, Vol. 112,12, 3693 -3704, 2008. 17. Motupally,S., Becker,A.J

  11. Strength and fatigue of NT551 silicon nitride and NT551 diesel exhaust valves

    SciTech Connect

    Andrews, M.J.; Werezczak, A.A.; Kirkland, T.P.; Breder, K.

    2000-02-01

    The content of this report is excerpted from Mark Andrew's Ph.D. Thesis (Andrews, 1999), which was funded by a DOE/OTT High Temperature Materials Laboratory Graduate Fellowship. It involves the characterization of NT551 and valves fabricated with it. The motivations behind using silicon nitride (Si{sub 3}N{sub 4}) as an exhaust valve for a diesel engine are presented in this section. There are several economic factors that have encouraged the design and implementation of ceramic components for internal combustion (IC) engines. The reasons for selecting the diesel engine valve for this are also presented.

  12. Can gallium dimer react effectively with three H 2 molecules to form digallane?

    NASA Astrophysics Data System (ADS)

    Moc, Jerzy

    2005-06-01

    Distinct routes of formation of digallane (4) Ga 2H 4 and digallane (6) Ga 2H 6 starting with gallium atoms/H 2 and gallium dimers/H 2 are compared based on the high level ab initio single-reference CCSD(T) and CASSCF based SOCI and hybrid density functional theory calculations of the mechanisms involved. The predicted reaction paths are of relevance to the recent matrix IR studies on the gallium hydrides, notably the extensive one by Wang and Andrews [X. Wang, L. Andrews, J. Phys. Chem. A 107 (2003) 11371] who isolated and assigned the series of mono- and digallium hydrides including the two digallanes.

  13. Design of Breakup Ice Control Structures

    DTIC Science & Technology

    2006-03-01

    ER D C/ CR R EL T R -0 6 -7 Design of Breakup Ice Control Structures Andrew M. Tuthill and James H. Lever March 2006 C ol d R eg...March 2006 Design of Breakup Ice Control Structures Andrew M. Tuthill and James H. Lever Cold Regions Research and Engineering Laboratory U.S. Army...ice control structure (ICS) is to retain a breakup ice run upstream of a traditional ice jam problem area and thereby miti- gate ice-jam flooding

  14. [Accessible Rural Housing.

    ERIC Educational Resources Information Center

    Baker, Nick, Ed.

    1995-01-01

    This issue of the quarterly newsletter "Rural Exchange" provides information and resources on accessible rural housing for the disabled. "Accessible Manufactured Housing Could Increase Rural Home Supply" (Nick Baker) suggests that incorporation of access features such as lever door handles and no-step entries into manufactured housing could help…

  15. Current Research in European Vocational Education and Human Resource Development. Proceedings of the Programme Presented by the Research Network on Vocational Education and Training (VETNET) at the European Conference of Educational Research (ECER) (4th, Lille, France, September 5-8, 2001).

    ERIC Educational Resources Information Center

    Manning, Sabine, Ed.; Dif, M'Hamed, Ed.

    These proceedings are comprised of 23 presentations on research in European vocational education and human resource development. Papers include "Developing Information and Communication Technology Capability in Higher Education in the United Kingdom (UK)" (Nick Boreham); "Methodological Issues in the Study of Organizational…

  16. Impact of In-channel Organic Debris on Fluvial Process and Channel Form

    DTIC Science & Technology

    1994-10-01

    Researchers : nick Wallerstein & Prof. C. R. Thorne Administrative Developments A 486DX PC has now been purchased in order to carry out survey data...survey in January 1995. Mr Wallerstein will travel out to Mississippi on 27/28th December and meet up with the field crew in Oxford to commence the three

  17. Affordances of Equality: Rancière, Emerging Media, and the New Amateur

    ERIC Educational Resources Information Center

    Thumlert, Kurt

    2015-01-01

    This article extends a recent educational engagement with the work of Jacques Rancière by linking his meditations on 19th-century worker emancipation to present cultural contexts and media forms. Taking Nick Prior's (2010) notion of the "new amateur" as point of departure, I argue that new media and attendant production contexts offer an…

  18. Self-primed isothermal amplification for genomic DNA detection of human papillomavirus.

    PubMed

    Lu, Wei; Yuan, Qingpan; Yang, Zhiliu; Yao, Bo

    2017-04-15

    Rolling circle amplification (RCA) is an isothermal amplification technique with high efficiency and perfect accuracy for nucleic acids detection. However, RCA technique suffers the limitation to detect short DNA or RNA molecules. For long nucleic acid molecules, enzymatic restriction as well as heat denaturation process is usually required, which makes the amplification not effective and strictly isothermal. In this article, a simple and efficient one-pot self-primed isothermal amplification (SIA) was developed for detection of genomic DNA directly based on the combination of nicking endonuclease assisted strand displacement amplification (SDA) and exponential RCA. In virtue of numerous nicking sites on the genome, a pre-amplification of the whole genome was performed through SDA with the specific cleaving of nicking endonuclease. Meanwhile, the single strand DNA with HPV target sequence generated from SDA could hybrid with the circle probe as a primer and trigger the exponential RCA as a result of the existence of nicking endonuclease. As the reaction temperature and enzyme were the same, the amplification could be operated in one pot. The reaction solution after amplification was added on the electrode for hybridization with the sulfydryl probe to achieve the electrochemical signal. Based on the isothermal amplification, genotyping of HPV 11, 16, 18 and the detection of HPV 18 in Hela cell line were attempted with satisfied results. This approach should be a promising tool for pathogene detection in clinical diagnostics and research.

  19. Electronic Paper Turns the Page.

    ERIC Educational Resources Information Center

    Mann, Charles C.

    2001-01-01

    Suggests that, rather than the electronic book, the technology that is most likely to transform reading and writing will be electronic paper (e-paper). Traces the evolution of e-paper from its prototype created by Xerox PARC's Nick Sheridon in 1975 to the E Ink/Lucent e-paper made from e-ink and plastic transistors. Highlights future…

  20. Preliminary Comparison of the T-11 Advanced Tactical Parachute System with the T-10D Parachute, Fort Bragg, North Carolina, June 2010-November 2011

    DTIC Science & Technology

    2011-12-01

    operations. CW3 Thompson (Corps Parachute Officer, XVIII Airborne Corps), CW4 Lewis (Division Parachute Officer, 82nd Airborne Division), Mr. Nick ...10, 2011. 3. Preventive Medicine. Washington DC: Headquarters, Department of the Army, Technical Report No. 40-5, 2007. 4. Hodge , J.G. An