Science.gov

Sample records for nick kettridge andrew

  1. Be Like Nick.

    ERIC Educational Resources Information Center

    Smith, Paul Chaat

    1996-01-01

    Discusses controversy surrounding books supposedly written by and about American Indians that are fabrications written by whites. Stresses the importance of moving away from American Indian literature that trivializes Indian culture and values, toward more realistic portrayals. Black Elk, whose first name was Nick, is an example of how cultural…

  2. Andrew: CMU's New Computing Environment.

    ERIC Educational Resources Information Center

    Zabowski, Susan

    1986-01-01

    Reviews the progress and problems associated with the development of Carnegie Mellon University's new computing and communications system, "Andrew." Describes the accomplishments and capacities of the system and provides examples of the programs developed for "Andrew." (ML)

  3. Reply to Andrew Wright

    ERIC Educational Resources Information Center

    White, John

    2005-01-01

    Andrew Wright has recently criticized an article penned by the author, which suggests that no good reasons have been given why religious education should be a compulsory school subject. In this article, the author explains the two misunderstandings Wright has about his position. First, Wright characterized the author's thesis as arguing "from the…

  4. Interview with Deborah Andrews

    ERIC Educational Resources Information Center

    Ryan, Cynthia

    2005-01-01

    This article presents an interview with Deborah Andrews about her experiences during her editorship of "Business Communication Quarterly." From June 1997 to March 2005, Debby served as editor of the journal, encouraging all readers to ask important questions about their work: How should we define business communication? On which disciplines and…

  5. Andrew W. S. In

    ERIC Educational Resources Information Center

    Nishimoto, Warren

    2007-01-01

    This article presents an interview with Andrew W. S. In, professor and dean in the University of Hawai'i's College of Education from 1951 to 1984. Born and raised in Honolulu, In attended Royal Elementary, Central Junior High, and McKinley High schools, graduating from McKinley in 1938. He then attended the University of Hawai'i Teachers College…

  6. Stories Along the Way: Andrew

    ERIC Educational Resources Information Center

    Kittle, Penny

    2009-01-01

    Andrew is among the forgotten, the written-off, the hopeless...at least to many of the adults in his life--police, teachers, even family. But when you see that glimmer, that potential, you can't turn your back. Andrew stands as a person, but also a metaphor for believing that each student has a chance to succeed.

  7. Mortality from Hurricane Andrew.

    PubMed

    Lew, E O; Wetli, C V

    1996-05-01

    Hurricane Andrew, a category 4 storm, made landfall in South Florida on August 24, 1992, and caused extensive structural and environmental damage. The Dade County Medical Examiner Department investigated 15 deaths directly related to the storm and another 15 natural deaths indirectly related to the storm. The aftermath of the hurricane continued to create circumstances that lead to 32 accidental deaths, five suicides, and four homicides over the next six months. Traffic fatalities due to uncontrolled intersections accounted for one-third of the post-storm accidental deaths. Dyadic deaths (homicide-suicide) doubled in rate for the six months following the storm. The limited number of direct hurricane deaths is attributed to advance storm warnings, its occurrence on a weekend, the storm's passage through less populated areas of the county, and the relatively modest amount of accompanying rainfall.

  8. Faces of Marshall: Erika Andrews

    NASA Video Gallery

    Several Marshall employees were interviewed as part of Marshall's 50th Anniversary activities. Human Resources Specialist Erika Andrews tells how she came to work at NASA as a specialist in organiz...

  9. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks.

    PubMed

    Vriend, Lianne E M; Prakash, Rohit; Chen, Chun-Chin; Vanoli, Fabio; Cavallo, Francesca; Zhang, Yu; Jasin, Maria; Krawczyk, Przemek M

    2016-06-20

    DNA double-strand breaks (DSBs) are known to be powerful inducers of homologous recombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR. Both DSB- and nick-induced HR ((nick)HR) are exploited in advanced genome-engineering approaches based on the bacterial RNA-guided nuclease Cas9. However, the mechanisms of (nick)HR are largely unexplored. Here, we applied Cas9 nickases to study (nick)HR in mammalian cells. We find that (nick)HR is unaffected by inhibition of major damage signaling kinases and that it is not suppressed by nonhomologous end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to trigger HR. Relative to a single nick, nicking both strands enhances HR, consistent with a DSB intermediate, even when nicks are induced up to ∼1kb apart. Accordingly, HR and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated. Our study advances the understanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena associated with Cas9-mediated genome editing.

  10. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks

    PubMed Central

    Vriend, Lianne E.M.; Prakash, Rohit; Chen, Chun-Chin; Vanoli, Fabio; Cavallo, Francesca; Zhang, Yu; Jasin, Maria; Krawczyk, Przemek M.

    2016-01-01

    DNA double-strand breaks (DSBs) are known to be powerful inducers of homologous recombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR. Both DSB- and nick-induced HR (nickHR) are exploited in advanced genome-engineering approaches based on the bacterial RNA-guided nuclease Cas9. However, the mechanisms of nickHR are largely unexplored. Here, we applied Cas9 nickases to study nickHR in mammalian cells. We find that nickHR is unaffected by inhibition of major damage signaling kinases and that it is not suppressed by nonhomologous end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to trigger HR. Relative to a single nick, nicking both strands enhances HR, consistent with a DSB intermediate, even when nicks are induced up to ∼1kb apart. Accordingly, HR and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated. Our study advances the understanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena associated with Cas9-mediated genome editing. PMID:27001513

  11. Benevolent Builder: Appraising Andrew Carnegie.

    ERIC Educational Resources Information Center

    Deitch, Joseph

    1984-01-01

    Discussion of Andrew Carnegie era in American public library history focuses on library buildings financed and built in Philadelphia, Pennsylvania. The building design which Carnegie developed, surviving Carnegie libraries in Philadelphia, procedures for obtaining a library, and comparison of architectural attractiveness of Carnegie libraries with…

  12. Libraries and Andrew Carnegie's Challenge.

    ERIC Educational Resources Information Center

    Gregorian, Vartan

    This essay by the president of the Carnegie Corporation of New York (reprinted from the 1998 annual report) opens by noting that the year 1999 marks the 100th anniversary of Andrew Carnegie's support for the planning and development of 65 branch libraries of the New York Public Library System, a gift that came to more than $5.2 million. Discussion…

  13. GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism

    PubMed Central

    Chan, Ying Wai; West, Stephen

    2015-01-01

    Holliday junctions (HJs) that physically link sister chromatids or homologous chromosomes are formed as intermediates during DNA repair by homologous recombination. Persistent recombination intermediates are acted upon by structure-selective endonucleases that are required for proper chromosome segregation at mitosis. Here, we have purified full-length human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is analogous to that exhibited by the prototypic HJ resolvase E. coli RuvC. We find that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordinated incisions that lead to the formation of ligatable nicked duplex products. As observed with RuvC, cleavage of the first strand is rate limiting, while second strand cleavage is rapid. In contrast to RuvC, however, GEN1 is largely monomeric in solution, but dimerizes on the HJ. Using HJs containing non-cleavable phosphorothioate-containing linkages in one strand, we show that the two incisions can be uncoupled and that the first nick occurs upon GEN1 dimerization at the junction. These results indicate that the mechanism of HJ resolution is largely conserved from bacteria to man, despite a lack of sequence homology between the resolvases. PMID:26578604

  14. The Impeachment of Andrew Johnson. Web Lesson.

    ERIC Educational Resources Information Center

    Constitutional Rights Foundation, Los Angeles, CA.

    This lesson presents the historical background of Abraham Lincoln's selection of Andrew Johnson as his running mate in the election of 1864. The lesson considers the climate in the U.S. Congress after President Lincoln's assassination. The details of the impeachment and trial of President Andrew Johnson are given. The lesson presents three…

  15. Psychiatric morbidity following Hurricane Andrew.

    PubMed

    David, D; Mellman, T A; Mendoza, L M; Kulick-Bell, R; Ironson, G; Schneiderman, N

    1996-07-01

    The nature of psychiatric morbidity in previously non-ill subjects from the area most affected by Hurricane Andrew was investigated at 6-12 months posthurricane. Preliminary associations of morbidity with personal and event-related risk factors were also determined. Fifty one percent (31/61) met criteria for a new-onset disorder, including posttraumatic stress disorder (PTSD) in 36%, major depression (MD) in 30%, and other anxiety disorders in 20%. Thirty four subjects (56%) had significant symptoms persisting beyond 6 months. Having sustained "severe damage" was the risk factor most strongly associated with outcome. Our data underscore the range of psychiatric morbidity related to a natural disaster, and suggest a relationship to chronic stressors.

  16. Obituary: Andrew Stephen Wilson, 1947-2008

    NASA Astrophysics Data System (ADS)

    Veilleux, Sylvain

    2009-01-01

    On 24 May 2008, Andrew Stephen Wilson passed away at the age of 61, in his home in Silver Spring, Maryland, from complications resulting from a painful spinal illness. Andrew was arguably one of the first truly multi-wavelength astronomers of his generation. His scientific work on active galactic nuclei [AGN] spanned the entire electromagnetic spectrum from the radio to the X-rays. Andrew was born in Doncaster, Yorkshire, England, on 26 March 1947. He was the younger of two brothers whose births were separated by the Second World War. His father, Norman, came from a relatively affluent family who were coal merchants. His mother, Mary, came from a less comfortable background, one of seven children, daughter of a skilled cabinet maker/French polisher, who went through a very hard time during the depression. As a teacher, she placed enormous value on hard work and education as a way of gaining advancement in life. When Andrew was four, the family moved to Skipton, a nice market town in the Yorkshire dales. Andrew went to a small village school until age eleven when he entered Ermysted's Grammar School. He was an enthusiastic soccer and cricket player. He never lost his enthusiasm for soccer and supported the local soccer team, Leeds United, for all his life. Andrew also followed the Yorkshire county cricket team. Andrew's interest in astronomy stemmed from the fact that at Ermysted's Grammar School someone donated a four-inch refracting telescope, so he and his friends used to go back in the evenings to investigate the rings of Saturn, the moons of Jupiter, and various nebulae. While an undergraduate at Cambridge, Andrew joined the astronomy club and ground an 8-inch mirror by hand as a part of a telescope that he set up in the backyard of his parents' house. Andrew spent hours observing with this telescope, and it was the wonder of the family. At Cambridge, Andrew obtained his bachelor's degree with first-class honors in 1969. During a short visit in London with his

  17. A Reanalysis of Hurricane Andrew's Intensity.

    NASA Astrophysics Data System (ADS)

    Landsea, Christopher W.; Franklin, James L.; McAdie, Colin J.; Beven, John L., II; Gross, James M.; Jarvinen, Brian R.; Pasch, Richard J.; Rappaport, Edward N.; Dunion, Jason P.; Dodge, Peter P.

    2004-11-01

    Hurricane Andrew of 1992 caused unprecedented economic devastation along its path through the Bahamas, southeastern Florida, and Louisiana. Damage in the United States was estimated to be $26 billion (in 1992 dollars), making Andrew one of the most expensive natural disasters in U.S. history. This hurricane struck southeastern Florida with maximum 1-min surface winds estimated in a 1992 poststorm analysis at 125 kt (64 m s-1). This original assessment was primarily based on an adjustment of aircraft reconnaissance flight-level winds to the surface.Based on recent advancements in the understanding of the eyewall wind structure of major hurricanes, the official intensity of Andrew was adjusted upward for five days during its track across the Atlantic Ocean and Gulf of Mexico by the National Hurricane Center Best Track Change Committee. In particular, Andrew is now assessed by the National Hurricane Center to be a Saffir Simpson Hurricane Scale category-5 hurricane (the highest intensity category possible) at its landfall in southeastern Florida, with maximum 1-min winds of 145 kt (75 m s-1). This makes Andrew only the third category-5 hurricane to strike the United States since at least 1900. Implications for how this change impacts society's planning for such extreme events are discussed.

  18. Congruences for the Andrews spt function.

    PubMed

    Ono, Ken

    2011-01-11

    Ramanujan-type congruences for the Andrews spt(n) partition function have been found for prime moduli 5 ≤ ℓ ≤ 37 in the work of Andrews [Andrews GE, (2008) J Reine Angew Math 624:133-142] and Garvan [Garvan F, (2010) Int J Number Theory 6:1-29]. We exhibit unexpectedly simple congruences for all ℓ≥5. Confirming a conjecture of Garvan, we show that if ℓ≥5 is prime and (-δ/ℓ) = 1, then spt[(ℓ2(ℓn+δ)+1)/24] ≡ 0 (mod ℓ). This congruence gives (ℓ - 1)/2 arithmetic progressions modulo ℓ(3) which support a mod ℓ congruence. This result follows from the surprising fact that the reduction of a certain mock theta function modulo ℓ, for every ℓ≥5, is an eigenform of the Hecke operator T(ℓ(2)).

  19. Obituary: Andrew Stephen Wilson, 1947-2008

    NASA Astrophysics Data System (ADS)

    Veilleux, Sylvain

    2009-01-01

    On 24 May 2008, Andrew Stephen Wilson passed away at the age of 61, in his home in Silver Spring, Maryland, from complications resulting from a painful spinal illness. Andrew was arguably one of the first truly multi-wavelength astronomers of his generation. His scientific work on active galactic nuclei [AGN] spanned the entire electromagnetic spectrum from the radio to the X-rays. Andrew was born in Doncaster, Yorkshire, England, on 26 March 1947. He was the younger of two brothers whose births were separated by the Second World War. His father, Norman, came from a relatively affluent family who were coal merchants. His mother, Mary, came from a less comfortable background, one of seven children, daughter of a skilled cabinet maker/French polisher, who went through a very hard time during the depression. As a teacher, she placed enormous value on hard work and education as a way of gaining advancement in life. When Andrew was four, the family moved to Skipton, a nice market town in the Yorkshire dales. Andrew went to a small village school until age eleven when he entered Ermysted's Grammar School. He was an enthusiastic soccer and cricket player. He never lost his enthusiasm for soccer and supported the local soccer team, Leeds United, for all his life. Andrew also followed the Yorkshire county cricket team. Andrew's interest in astronomy stemmed from the fact that at Ermysted's Grammar School someone donated a four-inch refracting telescope, so he and his friends used to go back in the evenings to investigate the rings of Saturn, the moons of Jupiter, and various nebulae. While an undergraduate at Cambridge, Andrew joined the astronomy club and ground an 8-inch mirror by hand as a part of a telescope that he set up in the backyard of his parents' house. Andrew spent hours observing with this telescope, and it was the wonder of the family. At Cambridge, Andrew obtained his bachelor's degree with first-class honors in 1969. During a short visit in London with his

  20. Nick Joaquin: A Portrait of the Existentialist as Filipino.

    ERIC Educational Resources Information Center

    Zialcita, Fernanado

    1990-01-01

    Nick Joaquin, a leading English-language writer in the Philippines, explores the cultural construction of self though fiction that centers on the lives of the Spanish-speaking elite. Joaquin seeks to interpret the self as a dynamic process, forever exploring, interrogating, and reaffirming itself against the background of Philippine culture. (25…

  1. Oxygen and Metastasis: A Conversation with Dr. Nick Restifo

    Cancer.gov

    Dr. Nick Restifo, a senior investigator in NCI’s Center for Cancer Research, discusses his recently published study finding that Oxygen, a molecule necessary for life, paradoxically aids cancer metastasis to the lung by impairing cancer-killing immune cells.

  2. Andrew's Aftermath: Hurricane "Saves" Miami Public Library.

    ERIC Educational Resources Information Center

    St. Lifer, Evan

    1994-01-01

    Examines the impact of Hurricane Andrew on the Miami-Dade Public Library System (MDPLS). Topics discussed include the community's response to the sudden lack of library services; the use of library branches as emergency relief centers and communications centers; library disaster policies; and visions for MDPLS under a new director. (LRW)

  3. Striking design suits St Andrews skyline.

    PubMed

    Bell, Paul

    2011-09-01

    Creating a new hospital that proves itself to be sustainable on a greenfield site is quite a challenge. Paul Bell, director at Ryder Architecture, describes how the St Andrews Community Hospital and Health Centre has been delivered to achieve just that for NHS Fife.

  4. Obituary: Andrew Lange (1957-2010)

    NASA Astrophysics Data System (ADS)

    Kamionkowski, Marc

    2011-12-01

    The worlds of physics and astrophysics were stunned to learn on 22 January 2010 that Andrew Lange, the Marvin L. Goldberger Professor of Physics at Caltech, had taken his own life the night before. He had succumbed to the severe depression that he had suffered from for many years, unbeknownst to even his closest colleagues. Lange will perhaps be best remembered as the co-leader of Boomerang, the balloon-borne experiment that provided the first high-angular-resolution map of the cosmic microwave background (CMB). And while this was certainly his most notable achievement, Andrew amassed a record of accomplishment as an instrumentalist, leader, mentor, and communicator that extended much further. Andrew was born in Urbana, Illinois on July 23, 1957, the son of an architect and a librarian, and raised primarily in Connecticut. His family and early friends remember him as a serious and extremely intelligent child and young man. Andrew Lange's lifelong interest in the CMB was nurtured as an undergraduate at Princeton University by David Wilkinson, and he recalled fondly a summer spent working with John Mather at Goddard Space Flight Center. Andrew Lange went to graduate school in physics at Berkeley where he worked in Paul Richards' group. Although his thesis project, the Berkeley-Nagoya rocket experiment, showed an anomalous sub-millimeter excess in the CMB spectrum that was shortly thereafter shown by a later flight of the same rocket and COBE-FIRAS to be incorrect, Lange's talents were recognized by the physics department at Berkeley who appointed him shortly after his PhD (1987) to their faculty. While on the Berkeley faculty, Andrew obtained early detections of the Sunyaev-Zeldovich effect, upper limits to small-angle CMB fluctuations, and important infrared constraints to the interstellar medium. He also led a pioneering instrument operating 300 mK detectors for a small infrared satellite experiment. This early work showed high ambition and daring, and it pioneered

  5. A History of the Andrew File System

    SciTech Connect

    2011-02-22

    Derrick Brashear and Jeffrey Altman will present a technical history of the evolution of Andrew File System starting with the early days of the Andrew Project at Carnegie Mellon through the commercialization by Transarc Corporation and IBM and a decade of OpenAFS. The talk will be technical with a focus on the various decisions and implementation trade-offs that were made over the course of AFS versions 1 through 4, the development of the Distributed Computing Environment Distributed File System (DCE DFS), and the course of the OpenAFS development community. The speakers will also discuss the various AFS branches developed at the University of Michigan, Massachusetts Institute of Technology and Carnegie Mellon University.

  6. Andrew shortens lifetime of Louisiana Barrier Islands

    NASA Astrophysics Data System (ADS)

    Bush, Susan

    Because the Isles Dernieres, a series of four barrier islands off the coast of Louisiana, have one of the most rapidly eroding shorelines in the world, geologists at the U.S. Geological Survey and the Louisiana Geological Survey have been monitoring erosion activity over the last several years, said Jeff Williams of the USGS in Reston, Va. Hurricane Andrew, which struck the state on August 26, caused severe erosional damage to these islands that has shortened their lifetimes.Before Andrew struck, geologists projected that Raccoon Island would disappear below sea level by the year 2001 and that Whiskey Island would disappear by 2016. Now, due to the severe erosion from Hurricane Andrew, the scientists claim that the islands may disappear before the turn of the century, and the other islands in the Dernieres chain are expected to follow suit within 2 decades. Raccoon, Whiskey, Trinity, and East islands make up the Isles Dernieres, which existed as one island, known as the Isle Derniere, before an 1856 hurricane and subsequent erosion.

  7. The human side of Hurricane Andrew

    SciTech Connect

    Marshall, R.; Callander, R.C.

    1994-12-31

    This paper examines the long-term psychological effects of the nation`s worst natural disaster on the employees of the Turkey Point nuclear power plant. It also examines the efforts made by plant personnel and company volunteers to aid employees` families affected by the storm. Despite significant damage at the plant, unit 4 was returned to service 5 weeks after the August 24, 1992, hurricane. Unit 3 was returned to service on December 3, 1992. Unit 3 was originally scheduled to start a refueling outage the day Hurricane Andrew struck. While plant personnel are still recovering from Andrew`s impact, the plant`s performance has never been better. On May 26, 1993, the plant completed a record-breaking 46-day refueling outage - 7 days ahead of schedule and $3 million under budget. Turkey Point`s recovery, return to service, and superior performance would not have been possible without the efforts of hundreds of employees who put their personal tragedies aside and focused on the common goal of the plant`s operation. To help employees with rebuilding their lives, the plant launched extensive assistance programs. Although the plant returned to normal operation, plant personnel continue to struggle in a community whose infrastructure (homes, schools, stores, etc.) have been almost eliminated.

  8. Children describe life after Hurricane Andrew.

    PubMed

    Coffman, S

    1994-01-01

    Hurricane Andrew, which devastated the south Florida coast in August 1992, left over 250,000 people homeless with multiple health and social problems. This nursing study explored the experiences of 17 children, ages 5 through 12, who lived in the geographic area of storm damage. Common experiences described by the children included remembering the storm, dealing with after-effects, and reestablishing a new life. In general, children described a sense of strangeness, articulated as "life is weird" after the hurricane. In addition to stressful responses, many positive reactions were described by children in the study, revealing that the disaster also had a maturing effect.

  9. The spt-function of Andrews.

    PubMed

    Folsom, Amanda; Ono, Ken

    2008-12-23

    Recently, Andrews introduced the function s(n) = spt(n) which counts the number of smallest parts among the integer partitions of n. We show that its generating function satisfies an identity analogous to Ramanujan's mock theta identities. As a consequence, we are able to completely determine the parity of s(n). Using another type of identity, one based on Hecke operators, we obtain a complete multiplicative theory for s(n) modulo 3. These congruences confirm unpublished conjectures of Garvan and Sellers. Our methods generalize to all integral moduli.

  10. Obituary: Andrew Lange (1957-2010)

    NASA Astrophysics Data System (ADS)

    Kamionkowski, Marc

    2011-12-01

    The worlds of physics and astrophysics were stunned to learn on 22 January 2010 that Andrew Lange, the Marvin L. Goldberger Professor of Physics at Caltech, had taken his own life the night before. He had succumbed to the severe depression that he had suffered from for many years, unbeknownst to even his closest colleagues. Lange will perhaps be best remembered as the co-leader of Boomerang, the balloon-borne experiment that provided the first high-angular-resolution map of the cosmic microwave background (CMB). And while this was certainly his most notable achievement, Andrew amassed a record of accomplishment as an instrumentalist, leader, mentor, and communicator that extended much further. Andrew was born in Urbana, Illinois on July 23, 1957, the son of an architect and a librarian, and raised primarily in Connecticut. His family and early friends remember him as a serious and extremely intelligent child and young man. Andrew Lange's lifelong interest in the CMB was nurtured as an undergraduate at Princeton University by David Wilkinson, and he recalled fondly a summer spent working with John Mather at Goddard Space Flight Center. Andrew Lange went to graduate school in physics at Berkeley where he worked in Paul Richards' group. Although his thesis project, the Berkeley-Nagoya rocket experiment, showed an anomalous sub-millimeter excess in the CMB spectrum that was shortly thereafter shown by a later flight of the same rocket and COBE-FIRAS to be incorrect, Lange's talents were recognized by the physics department at Berkeley who appointed him shortly after his PhD (1987) to their faculty. While on the Berkeley faculty, Andrew obtained early detections of the Sunyaev-Zeldovich effect, upper limits to small-angle CMB fluctuations, and important infrared constraints to the interstellar medium. He also led a pioneering instrument operating 300 mK detectors for a small infrared satellite experiment. This early work showed high ambition and daring, and it pioneered

  11. Hurricane Andrew: Impact on hazardous waste management

    SciTech Connect

    Kastury, S.N. )

    1993-03-01

    On August 24, 1992, Hurricane Andrew struck the eastern coast of South Florida with winds of 140 mph approximately and a storm surge of 15 ft. The Florida Department of Environmental Regulation finds that the Hurricane Andrew caused a widespread damage throughout Dade and Collier County as well as in Broward and Monroe County and has also greatly harmed the environment. The Department has issued an emergency final order No. 92-1476 on August 26, 1992 to address the environmental cleanup and prevent any further spills of contaminants within the emergency area. The order authorizes the local government officials to designate certain locations in areas remote from habitation for the open burning in air certain incinerators of hurricane generated yard trash and construction and demolition debris. The Department staff has assisted the county and FEMA staff in establishing procedures for Hazardous Waste Management, Waste Segregation and disposal and emergency responses. Local governments have issued these burn permits to public agencies including FDOT and Corps of Engineering (COE). Several case studies will be discussed on the Hazardous Waste Management at this presentation.

  12. Structural Basis for Nick Recognition by a Minimal Pluripotent DNA Ligase

    SciTech Connect

    Nair,P.; Nandakumar, J.; Smith, P.; Odell, M.; Lima, C.; Shuman, S.

    2007-01-01

    Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-{angstrom} crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO{sub 4} nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a {beta}-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO{sub 4} termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

  13. 76 FR 48168 - Andrew K. Choi: Debarment Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-08

    ... HUMAN SERVICES Food and Drug Administration Andrew K. Choi: Debarment Order AGENCY: Food and Drug... the Federal Food, Drug, and Cosmetic Act (the FD&C Act) debarring Andrew K. Choi, M.D. for 4 years... K. Choi has been convicted of a misdemeanor under Federal law for conduct relating to the...

  14. A ligation-independent cloning method using nicking DNA endonuclease.

    PubMed

    Yang, Jie; Zhang, Zhihong; Zhang, Xin A; Luo, Qingming

    2010-11-01

    Using nicking DNA endonuclease (NiDE), we developed a novel technique to clone DNA fragments into plasmids. We created a NiDE cassette consisting of two inverted NiDE substrate sites sandwiching an asymmetric four-base sequence, and NiDE cleavage resulted in 14-base single-stranded termini at both ends of the vector and insert. This method can therefore be used as a ligation-independent cloning strategy to generate recombinant constructs rapidly. In addition, we designed and constructed a simple and specific vector from an Escherichia coli plasmid back-bone to complement this cloning method. By cloning cDNAs into this modified vector, we confirmed the predicted feasibility and applicability of this cloning method. PMID:21091446

  15. Introduction of Dr. Andrew V Schally

    PubMed Central

    Mendoza-Valdés, Arturo

    2015-01-01

    I first met Dr. Andrew V Schally (PhD, MDhc (Multi), DSc, Distinguished Medical Research Scientist, U.S. Department of Veterans Affairs Professor of Pathology and Department of Medicine,
Miller School of Medicine, University of Miami, Miami, Florida, USA) many years ago, probably around the beginning of the 1990's in one of his visits to Mexico City (Figure 1). He has many friends in my country since some of the investigations that led to the development of the LHRH agonists were made in a couple of Mexican hospitals in collaboration with some outstanding Mexican physicians that I will mention later. In that time, I was the head of the Department of Urology of the Mexican National Cancer Institute and our Director, Dr. Jaime de la Garza, invited him for a meeting. I was surprised by his humbleness, intelligence and easy going personality, in spite of being a Nobel Prize scientist. PMID:26112485

  16. 9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW PHOTOGRAPHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW - PHOTOGRAPHER SEPT. 7, 1936 BEAM, CORNICE, AND CEILING ORNAMENT IN FRONT HALL - Sorrel-Weed House, 6 West Harris Street, Savannah, Chatham County, GA

  17. 2. Historic American Buildings Survey L. D. Andrew, Photographer enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer enlarged Photographed by Harold Bush-Brown Dec. 17, 1936 PORTION OF FRONT - Griffin-Mott House, Mott Street & Front Avenue, Columbus, Muscogee County, GA

  18. 7. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 CORNER OF WEST FRONT ROOM (SHOWING CORNICE AND WINDOW HEAD) - Wayne-Gordon House, 10 East Oglethorpe Avenue, Savannah, Chatham County, GA

  19. 3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 CENTRAL PORTION OF REAR (Showing backsteps, stoop & stairhall, window) - Wayne-Gordon House, 10 East Oglethorpe Avenue, Savannah, Chatham County, GA

  20. 2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From photo of Miss Edith Johnston, Savannah, Ga. 1936 VIEW OF FRONT AND RIGHT SIDE (Restoration 1936) - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  1. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From Photo of Miss Edith Johnston's, Savannah, Ga. 1936 VIEW OF FRONT AND SIDE (Before Restoration, 1936). - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  2. 4. Historic American Buildings Survey L. D. Andrew Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey L. D. Andrew - Photographer June, 5, 1936 GARDEN LOOKING S.E. FROM SECOND STORY WINDOW - Kolb-Pou-Newton House, 218 South Second Street, Madison, Morgan County, GA

  3. 13. Circa 1880 view (from the north) across Andrew Hallidie ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Circa 1880 view (from the north) across Andrew Hallidie suspension bridge. Source: Searles Library, Nevada City, California. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA

  4. 5. Historic American Buildings Survey L. D. Andrew, Photographer From ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer From photograph furnished by Miss Edith D. Johnston, Savannah, Ga. Sept. 5, 1936 RED BRICK CHURCH (FORMER BUILDING 1811- 1837) - Christ Church (Episcopal), 28 Bull Street, Savannah, Chatham County, GA

  5. 2. Historic American Buildings Survey L. D. Andrew, Photographer, Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer, Enlarged Photographed by Harold Bush-Brown Nov. 14, 1936 VIEW OF EASTERN SLAVE CABIN - Bass Place (Slave Cabins), Columbus, Muscogee County, GA

  6. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photographed by Harold Bush-Brown Nov. 14, 1936 GENERAL VIEW OF SLAVE CABINS - Bass Place (Slave Cabins), Columbus, Muscogee County, GA

  7. 6. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 27, 1936 CAP AND ENTABLATURE OF ENTRANCE PORTICO - Jerry Cowles House, 4596 Rivoli Drive (moved from 519 Walnut Street), Macon, Bibb County, GA

  8. 2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM MORRISON'S CAF? (CORNER OF WHITAKER & CONGRESS) SHOWING S. FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  9. 3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM CORNER OF WHITAKER AND ST JULIAN SHOWING NORTH FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  10. 1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged by) Aug. 6, 1936 Photographed by Harold Bush-Brown SIDE VIEW - Covered Bridge, Spanning Soap Creek, Atlanta, Fulton County, GA

  11. 6. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 1936 From Photographs from Misses Baber-Blackshear, Macon, Georgia ARCH AND STAIRHALL - Baber House, Walnut Street, Macon, Bibb County, GA

  12. 2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 1936 From Photograph furnished by W. Elliott Dunwody, Jr. Arch't., Macon, Ga. ENTRANCE STOOP AND DOORWAY - Baber House, Walnut Street, Macon, Bibb County, GA

  13. 3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 PANELS AND VAULTED CEILING IN DRAWING ROOM, SECOND FLOOR - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  14. 5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 DETAIL OF DOUBLE OPENING WITH IRON GRATING IN THIRD STORY REAR ROOM - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  15. Remote sensing for hurricane Andrew impact assessment

    NASA Technical Reports Server (NTRS)

    Davis, Bruce A.; Schmidt, Nicholas

    1994-01-01

    Stennis Space Center personnel flew a Learjet equipped with instrumentation designed to acquire imagery in many spectral bands into areas most damaged by Hurricane Andrew. The calibrated airborne multispectral scanner (CAMS), a NASA-developed sensor, and a Zeiss camera acquired images of these areas. The information derived from the imagery was used to assist Florida officials in assessing the devastation caused by the hurricane. The imagery provided the relief teams with an assessment of the debris covering roads and highways so cleanup plans could be prioritized. The imagery also mapped the level of damage in residential and commercial areas of southern Florida and provided maps of beaches and land cover for determination of beach loss and vegetation damage, particularly the mangrove population. Stennis Space Center personnel demonstrated the ability to respond quickly and the value of such response in an emergency situation. The digital imagery from the CAMS can be processed, analyzed, and developed into products for field crews faster than conventional photography. The resulting information is versatile and allows for rapid updating and editing. Stennis Space Center and state officials worked diligently to compile information to complete analyses of the hurricane's impact.

  16. MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

    PubMed Central

    Anderson, Sarah; Poudel, Kumud Raj; Roh-Johnson, Minna; Brabletz, Thomas; Yu, Ming; Borenstein-Auerbach, Nofit; Grady, William N.; Bai, Jihong; Moens, Cecilia B.; Eisenman, Robert N.; Conacci-Sorrell, Maralice

    2016-01-01

    MYC-nick is a cytoplasmic, transcriptionally inactive member of the MYC oncoprotein family, generated by a proteolytic cleavage of full-length MYC. MYC-nick promotes migration and survival of cells in response to chemotherapeutic agents or withdrawal of glucose. Here we report that MYC-nick is abundant in colonic and intestinal tumors derived from mouse models with mutations in the Wnt, TGF-β, and PI3K pathways. Moreover, MYC-nick is elevated in colon cancer cells deleted for FBWX7, which encodes the major E3 ligase of full-length MYC frequently mutated in colorectal cancers. MYC-nick promotes the migration of colon cancer cells assayed in 3D cultures or grown as xenografts in a zebrafish metastasis model. MYC-nick accelerates migration by activating the Rho GTPase Cdc42 and inducing fascin expression. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration. PMID:27566402

  17. Force and twist dependence of RepC nicking activity on torsionally-constrained DNA molecules

    PubMed Central

    Pastrana, Cesar L.; Carrasco, Carolina; Akhtar, Parvez; Leuba, Sanford H.; Khan, Saleem A.; Moreno-Herrero, Fernando

    2016-01-01

    Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires sequence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding of the duplex prior to subsequent leading strand DNA synthesis. Nicking is performed by a replication-initiation protein (Rep) that directly binds to the plasmid double-stranded origin and remains covalently bound to its substrate 5′-end via a phosphotyrosine linkage. It has been proposed that the inverted DNA sequences at the nick site form a cruciform structure that facilitates DNA cleavage. However, the role of Rep proteins in the formation of this cruciform and the implication for its nicking and religation functions is unclear. Here, we have used magnetic tweezers to directly measure the DNA nicking and religation activities of RepC, the replication initiator protein of plasmid pT181, in plasmid sized and torsionally-constrained linear DNA molecules. Nicking by RepC occurred only in negatively supercoiled DNA and was force- and twist-dependent. Comparison with a type IB topoisomerase in similar experiments highlighted a relatively inefficient religation activity of RepC. Based on the structural modeling of RepC and on our experimental evidence, we propose a model where RepC nicking activity is passive and dependent upon the supercoiling degree of the DNA substrate. PMID:27488190

  18. MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation.

    PubMed

    Anderson, Sarah; Poudel, Kumud Raj; Roh-Johnson, Minna; Brabletz, Thomas; Yu, Ming; Borenstein-Auerbach, Nofit; Grady, William N; Bai, Jihong; Moens, Cecilia B; Eisenman, Robert N; Conacci-Sorrell, Maralice

    2016-09-13

    MYC-nick is a cytoplasmic, transcriptionally inactive member of the MYC oncoprotein family, generated by a proteolytic cleavage of full-length MYC. MYC-nick promotes migration and survival of cells in response to chemotherapeutic agents or withdrawal of glucose. Here we report that MYC-nick is abundant in colonic and intestinal tumors derived from mouse models with mutations in the Wnt, TGF-β, and PI3K pathways. Moreover, MYC-nick is elevated in colon cancer cells deleted for FBWX7, which encodes the major E3 ligase of full-length MYC frequently mutated in colorectal cancers. MYC-nick promotes the migration of colon cancer cells assayed in 3D cultures or grown as xenografts in a zebrafish metastasis model. MYC-nick accelerates migration by activating the Rho GTPase Cdc42 and inducing fascin expression. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration. PMID:27566402

  19. NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells

    SciTech Connect

    Shoji, Wataru; Suenaga, Yusuke; Kaneko, Yoshiki; Islam, S.M. Rafiqul; Alagu, Jennifer; Yokoi, Sana; Nio, Masaki; Nakagawara, Akira

    2015-06-05

    NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas. - Highlights: • NCYM promotes cleavages of MYC and MYCN to produce Myc-nick in vitro. • NCYM increases Myc-nick production in MYCN-amplified neuroblastoma cells. • NCYM knockdown decreases Myc-nick production and induces apoptosis at G2/M phase.

  20. Interview: Professor Andrew Feinberg speaks to Epigenomics.

    PubMed

    Feinberg, Andrew

    2009-10-01

    Andrew Feinberg studied mathematics and humanities at Yale University (CT, USA) in the Directed Studies honors program, and he received his BA (1973) and MD (1976) from the accelerated medical program at Johns Hopkins University (MD, USA), as well as an MPH from Johns Hopkins (1981). He performed a postdoctoral fellowship in developmental biology at the University of California, San Diego (UCSD, CA, USA), clinical training in medicine and medical genetics at the University of Pennsylvania (PA, USA) and genetics research with Bert Vogelstein at Johns Hopkins, discovering altered DNA methylation in human cancer. Dr Feinberg continued to perform seminal work in cancer epigenetics as a Howard Hughes investigator at the University of Michigan (MI, USA), discovering human imprinted genes and loss of imprinting in cancer, and the molecular basis of Beckwith-Wiedemann syndrome. He returned to John Hopkins in 1994 as King Fahd Professor of Medicine, Molecular Biology & Genetics and Oncology, and he holds an Adjunct Professorship at the Karolinska Institute in Sweden. Dr Feinberg is Director of the Center for Epigenetics, a National Human Genome Research Institute-designated Center of Excellence in Genome Sciences. The Center is pioneering genome-scale tools in molecular, statistical and epidemiological epigenetics, and is applying them to the study of cancer, neuropsychiatric disease and aging. As part of the center, Dr Feinberg has organized a highly innovative program to bring gifted minority high-school students into genetics and genomics. Dr Feinberg has also invented a number of widely used molecular tools, including random priming. His honors include election to the American Society for Clinical Investigation, the Association of American Physicians, the Institute of Medicine of the National Academy of Sciences, and the American Academy of Arts and Sciences, as well as membership on the ISI most-cited authors list, a MERIT Award of the National Cancer Institute, a

  1. Nick Ransford: 'There is a job to do so let's get on with it'.

    PubMed

    Ransford, Nick; Doherty, Ruth

    2014-03-01

    Nick Ransford is a consultant in special care dentistry at Birmingham Community Healthcare NHS Trust. He has over 25 years' experience working with adults with disabilities and medical conditions across the spectrum.

  2. PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

    PubMed Central

    Grundy, Gabrielle J.; Polo, Luis M.; Zeng, Zhihong; Rulten, Stuart L.; Hoch, Nicolas C.; Paomephan, Pathompong; Xu, Yingqi; Sweet, Steve M.; Thorne, Alan W.; Oliver, Antony W.; Matthews, Steve J.; Pearl, Laurence H.; Caldecott, Keith W.

    2016-01-01

    PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break. PMID:27530147

  3. Nick Ransford: 'There is a job to do so let's get on with it'.

    PubMed

    Ransford, Nick; Doherty, Ruth

    2014-03-01

    Nick Ransford is a consultant in special care dentistry at Birmingham Community Healthcare NHS Trust. He has over 25 years' experience working with adults with disabilities and medical conditions across the spectrum. PMID:24603240

  4. An effective automated system for grading severity of retinal arteriovenous nicking in colour retinal images.

    PubMed

    Roy, Pallab Kanti; Nguyen, Uyen T V; Bhuiyan, Alauddin; Ramamohanarao, Kotagiri

    2014-01-01

    Retinal arteriovenous (AV) nicking is a precursor for hypertension, stroke and other cardiovascular diseases. In this paper, an effective method is proposed for the analysis of retinal venular widths to automatically classify the severity level of AV nicking. We use combination of intensity and edge information of the vein to compute its widths. The widths at various sections of the vein near the crossover point are then utilized to train a random forest classifier to classify the severity of AV nicking. We analyzed 47 color retinal images obtained from two population based studies for quantitative evaluation of the proposed method. We compare the detection accuracy of our method with a recently published four class AV nicking classification method. Our proposed method shows 64.51% classification accuracy in-contrast to the reported classification accuracy of 49.46% by the state of the art method. PMID:25571443

  5. T98G glioma cells have nicks in DNA in quiescent phase

    SciTech Connect

    Taki, Takuyu; Arita, Norio; Hayakawa, Toru; Ohnishi, Takanori; Izumoto, Shuichi; Yamamoto, Hiroshi; Mogami, Heitaro )

    1990-10-01

    Human glioblastoma-derived cell line, T98G, is arrested in the G, phase of the cell cycle when serum is deprived. Using this cell line, the authors investigated the relation between the cell cycle and DNA single-stranded breaks, nicks,' by an in situ nick-translation method. When T98G cells were cultured without serum for 60 hours, many small cells with condensed chromatin and scanty cytoplasm appeared. These small cells that were immunohistochemically considered to be in the G{sub 0} or early G{sub 1} phase had many nicks in DNA. When serum was added, these small cells with nicks disappeared within 1 to 4 hours. VP-16, a DNA topoisomerase II inhibitor, delayed the disappearance of these small cells with nicks. This indicated that the action of DNA topoisomerase II on the chromatin is required to repair nicks in T98G glioma cells and to promote the progression from the quiescent to the proliferating phase.

  6. Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi

    PubMed Central

    Unciuleac, Mihaela-Carmen; Shuman, Stewart

    2015-01-01

    The proteome of the amoebo-flagellate protozoan Naegleria gruberi is rich in candidate RNA repair enzymes, including 15 putative RNA ligases, one of which, NgrRnl, is a eukaryal homolog of Deinococcus radiodurans RNA ligase, DraRnl. Here we report that purified recombinant NgrRnl seals nicked 3′-OH/5′-PO4 duplexes in which the 3′-OH strand is RNA. It does so via the “classic” ligase pathway, entailing reaction with ATP to form a covalent NgrRnl–AMP intermediate, transfer of AMP to the nick 5′-PO4, and attack of the RNA 3′-OH on the adenylylated nick to form a 3′–5′ phosphodiester. Unlike members of the four known families of ATP-dependent RNA ligases, NgrRnl lacks a carboxy-terminal appendage to its nucleotidyltransferase domain. Instead, it contains a defining amino-terminal domain that we show is important for 3′-OH/5′-PO4 nick-sealing and ligase adenylylation, but dispensable for phosphodiester synthesis at a preadenylylated nick. We propose that NgrRnl, DraRnl, and their homologs from diverse bacteria, viruses, and unicellular eukarya comprise a new “Rnl5 family” of nick-sealing ligases with a signature domain organization. PMID:25740837

  7. Appreciating Unity in Diversity: An Interview with Andrew Solomon

    ERIC Educational Resources Information Center

    Peters, Dane L.

    2014-01-01

    The theme of the AMS 2014 Annual Conference is "Unity in Diversity," a concept that also describes the work of conference keynote speaker Andrew Solomon. Solomon is a writer and lecturer on psychology and politics; winner of the National Book Award; and an activist for LGBT [lesbian, gay, bisexual, and transgender] rights, mental health,…

  8. Adolescent Responses to the MacAndrew Alcoholism Scale.

    ERIC Educational Resources Information Center

    Wolfson, Karen P.; Erbaugh, Susan E.

    1984-01-01

    Examined the ability of the MacAndrew Alcoholism Scale (MAC) to indicate drug abuse in high school students (N=135), adolescent psychiatric patients (N=171), and residents of a drug abuse treatment center (N=100). Results suggested the MAC can help discriminate between groups of adolescents with and without significant drug abuse. (JAC)

  9. 4. Historic American Buildings Survey L. D. Andrew, Photographer Sept. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey L. D. Andrew, Photographer Sept. 5, 1936 Photograph from photograph furnished by Miss Edith D. Johnston, Savannah, GA. VIEW OF CHANCEL IN CHRIST CHURCH BEFORE DEMOLITION photo ABOUT 1870 - Christ Church (Episcopal), 28 Bull Street, Savannah, Chatham County, GA

  10. The Mental Health Counselor's Role in Hurricane Andrew.

    ERIC Educational Resources Information Center

    Dingman, Robert L.

    1995-01-01

    Discusses the effects of Hurricane Andrew on disaster workers, followed by some reported experiences of workers as well as victims. Background on natural disasters in general is given, along with information about crisis intervention. Discusses mental health interventions and various skills needed by disaster mental health counselors. (Author/KW)

  11. Hurricane Andrew: Parent Conflict As a Moderator of Children's Adjustment.

    ERIC Educational Resources Information Center

    Wasserstein, Shari B.; La Greca, Annette M.

    1998-01-01

    Three months after Hurricane Andrew, 89 children in grades four to six who lived in two-parent families and had experienced the hurricane were tested. Children's symptoms of posttraumatic stress disorder were related to their perceptions of parental conflict; this relationship was stronger for Hispanic children than for other ethnic groups.…

  12. To Nick or Not to Nick: Comparison of I-SceI Single- and Double-Strand Break-Induced Recombination in Yeast and Human Cells

    PubMed Central

    Katz, Samantha S.; Gimble, Frederick S.; Storici, Francesca

    2014-01-01

    Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand break (DSB) at or near the site of correction, but repair of the break via non-homologous end-joining without using the homologous template can lead to deleterious genomic changes such as in/del mutations, or chromosomal rearrangements. By contrast, generation of a DNA single-strand break (SSB), or nick, can stimulate gene correction without the problems of DSB repair because the uncut DNA strand acts as a template to permit healing without alteration of genetic material. Here, we examine the ability of a nicking variant of the I-SceI endonuclease (K223I I-SceI) to stimulate gene targeting in yeast Saccharomyces cerevisiae and in human embryonic kidney (HEK-293) cells. K223I I-SceI is proficient in both yeast and human cells and promotes gene correction up to 12-fold. We show that K223I I-SceI-driven recombination follows a different mechanism than wild-type I-SceI-driven recombination, thus indicating that the initial DNA break that stimulates recombination is not a low-level DSB but a nick. We also demonstrate that K223I I-SceI efficiently elevates gene targeting at loci distant from the break site in yeast cells. These findings establish the capability of the I-SceI nickase to enhance recombination in yeast and human cells, strengthening the notion that nicking enzymes could be effective tools in gene correction strategies for applications in molecular biology, biotechnology, and gene therapy. PMID:24558436

  13. Parafermionic derivation of Andrews-type multiple sums

    NASA Astrophysics Data System (ADS)

    Jacob, P.; Mathieu, P.

    2005-09-01

    A multi-parafermion basis of states for the {{\\bb Z}}_k parafermionic models is derived. Its generating function is constructed by elementary steps. It corresponds to the Andrews multiple sum which enumerates partitions whose parts separated by the distance k - 1 differ by at least 2. Two analogous bases are derived for graded parafermions; one of these entails a new expression for their fermionic characters.

  14. Injuries and illnesses related to Hurricane Andrew--Louisiana, 1992.

    PubMed

    1993-04-01

    On August 26, 1992, Hurricane Andrew struck Louisiana. On August 24, in anticipation of hurricane-related injuries and illnesses, the Office of Public Health (OPH), Louisiana Department of Health and Hospitals, in cooperation with hospital emergency room (ER) and public utility personnel and coroners, established an active emergency surveillance system in 19 parishes to monitor these events. This report summarizes the findings from this emergency surveillance system.

  15. A pioneer of tropical medicine worldwide: Andrew Balfour, of Khartoum

    PubMed Central

    2013-01-01

    This is an archival account of the career of Sir Andrew Balfour in Khartoum, Sudan during the period 1902 to 1913. As the first director of the Wellcome Tropical Research Laboratories in Khartoum during the period, Andrew Balfour was tasked with establishing the laboratories and at the same time he was engaged in founding the health services in Khartoum. Balfour worked in close collaboration and support from Henry Wellcome and Reginald Wingate, the Governor General of the Sudan. The energetic and meticulous sanitary work of Balfour had a remarkable impact, with Khartoum declared mosquito-free by 1910. Establishing a research base in the laboratories was met with many challenges but eventually Balfour managed to recruit a team of dedicated researchers and to produce well-circulated publications in tropical medicine. Balfour’s work in Khartoum later lead him to a distinguished career in tropical medicine. In 1923 he was appointed the first Director of London School of Hygiene and Tropical Medicine. He was also elected President of the Royal Society of Tropical Medicine and Hygiene (1925–27). Sir Andrew Balfour, KCMG, CB, LL D (1873–1931) PMID:27493361

  16. A pioneer of tropical medicine worldwide: Andrew Balfour, of Khartoum.

    PubMed

    Adeel, Ahmed A A

    2013-01-01

    This is an archival account of the career of Sir Andrew Balfour in Khartoum, Sudan during the period 1902 to 1913. As the first director of the Wellcome Tropical Research Laboratories in Khartoum during the period, Andrew Balfour was tasked with establishing the laboratories and at the same time he was engaged in founding the health services in Khartoum. Balfour worked in close collaboration and support from Henry Wellcome and Reginald Wingate, the Governor General of the Sudan. The energetic and meticulous sanitary work of Balfour had a remarkable impact, with Khartoum declared mosquito-free by 1910. Establishing a research base in the laboratories was met with many challenges but eventually Balfour managed to recruit a team of dedicated researchers and to produce well-circulated publications in tropical medicine. Balfour's work in Khartoum later lead him to a distinguished career in tropical medicine. In 1923 he was appointed the first Director of London School of Hygiene and Tropical Medicine. He was also elected President of the Royal Society of Tropical Medicine and Hygiene (1925-27). Sir Andrew Balfour, KCMG, CB, LL D (1873 -1931).

  17. A new evidence for DNA nicking property of amyloid beta-peptide (1-42): relevance to Alzheimer's disease.

    PubMed

    Suram, A; Hegde, M L; Rao, K S J

    2007-07-15

    Alzheimer's disease (AD) is a complex neurodegenerative disorder with a progressive mental deterioration manifested by memory loss. No definite etiology has been established for AD to date. Amyloid beta (Abeta) protein plays a central role in the pathology of AD through multiple pathways like oxidative stress, apoptosis etc. Recently, our laboratory first time has evidenced localization of Abeta immunoreactivity in apoptotic nuclei of degenerating AD brain hippocampal neurons and also showed that Abeta (1-42) binds and alters the helicity of DNA. The present study provided fundamental data on DNA nicking induced by Abeta. The results showed that Abeta (1-42) has DNA nicking activity similar to nucleases. Further, magnesium ion (1mM) enhanced DNA nicking activity of Abeta. The data on Abeta solution stability on DNA nicking revealed that the oligomers of Abeta (1-42) peptides showed more DNA nicking activity compared to monomers and fibrillar forms. The nuclease specific inhibitor aurintricarboxylic acid prevented the DNA nicking property of Abeta. Transmission electron microscopy (TEM) studies revealed that Abeta causes open circular and linear forms in supercoiled DNA and also clearly evidenced the physical association of protein-DNA complex. The above data indicated that Abeta mimics endonuclease behavior. Our finding of DNA nicking activity of Abeta peptides has biological significance in terms of causing direct DNA damage.

  18. The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.

    PubMed Central

    Taylor, J W; Schmidt, W; Cosstick, R; Okruszek, A; Eckstein, F

    1985-01-01

    The RF IV form of M13 DNA was synthesized enzymatically in vitro, using the viral (+)strand as template, to contain phosphorothioate-modified internucleotidic linkages of the Rp configuration on the 5' side of every base of a particular type in the newly-synthesized (-)strand. Twenty nine restriction enzymes were then tested for their reactions with the appropriate modified DNA types having a phosphorothioate linkage placed exactly at the cleavage site(s) of these enzymes in the (-)strand. Eleven of the seventeen restriction enzymes tested that had recognition sequences of five bases or more could be used to convert the phosphorothioate DNA entirely into the nicked form, either by simply allowing the reaction to go to completion with excess enzyme (Ava I, Ava II, Ban II, Hind II, Nci I, Pst I or Pvu I) or by stopping the reaction at the appropriate time before the nicked DNA is linearized (Bam HI, Bgl I, Eco RI or Hind III). Only modification of the exact cleavage site in the (-)strand could block linearization by the first class of enzymes. The results presented imply that the restriction enzyme-directed nicking of phosphorothioate M13 DNA occurs exclusively in the (+)strand. Images PMID:3001649

  19. Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease

    PubMed Central

    Xu, Shuang-yong; Gupta, Yogesh K.

    2013-01-01

    Many bacteriophage and prophage genomes encode an HNH endonuclease (HNHE) next to their cohesive end site and terminase genes. The HNH catalytic domain contains the conserved catalytic residues His-Asn-His and a zinc-binding site [CxxC]2. An additional zinc ribbon (ZR) domain with one to two zinc-binding sites ([CxxxxC], [CxxxxH], [CxxxC], [HxxxH], [CxxC] or [CxxH]) is frequently found at the N-terminus or C-terminus of the HNHE or a ZR domain protein (ZRP) located adjacent to the HNHE. We expressed and purified 10 such HNHEs and characterized their cleavage sites. These HNHEs are site-specific and strand-specific nicking endonucleases (NEase or nickase) with 3- to 7-bp specificities. A minimal HNH nicking domain of 76 amino acid residues was identified from Bacillus phage γ HNHE and subsequently fused to a zinc finger protein to generate a chimeric NEase with a new specificity (12–13 bp). The identification of a large pool of previously unknown natural NEases and engineered NEases provides more ‘tools’ for DNA manipulation and molecular diagnostics. The small modular HNH nicking domain can be used to generate rare NEases applicable to targeted genome editing. In addition, the engineered ZF nickase is useful for evaluation of off-target sites in vitro before performing cell-based gene modification. PMID:23125367

  20. Evaluation of nicked human chorionic gonadotropin content in clinical specimens by a specific immunometric assay.

    PubMed

    Kovalevskaya, G; Birken, S; Kakuma, T; Schlatterer, J; O'Connor, J F

    1999-01-01

    We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb to choriocarcinoma-derived hCGn (C5) and a second mAb developed from immunization with normal-pregnancy hCGn. This assay was used to evaluate hCGn profiles in normal, in vitro fertilization, Down syndrome, and ectopic pregnancies. In all pregnancies, hCGn was usually present in much lower concentrations than the non-nicked hCG isoform. Our results suggest that some form of physical separation from the overwhelming quantities of non-nicked hCG present in clinical specimens will be required before accurate immunochemical estimations of hCGn can be made. PMID:9895340

  1. Can unequal be more fair? A response to Andrew Avins.

    PubMed

    Edwards, S J; Braunholtz, D A

    2000-06-01

    In this paper, we respond to Andrew Avins's recent review of methods whose use he advocates in clinical trials, to make them more ethical. He recommends in particular, "unbalanced randomisation". However, we argue that, before such a recommendation can be made, it is important to establish why unequal randomisation might offer ethical advantages over equal randomisation, other things being equal. It is important to make a pragmatic distinction between trials of treatments that are already routinely available and trials of restricted treatments. We conclude that unequal randomisation could, indeed, be an ethical compromise between protecting the interests of participants and those of society. PMID:10860209

  2. ANDREWS MOUNTAIN, MAZOURKA, AND PAIUTE ROADLESS AREAS, CALIFORNIA.

    USGS Publications Warehouse

    McKee, Edwin H.; Schmauch, Steven W.

    1984-01-01

    On the basis of a mineral survey, local areas near and within the Andrews Mountain, Mazourka, and Paiute Roadless Areas, California have probable and substantiated mineral-resource potential. The principal metallic mineral resources in these roadless areas are gold, copper, and silver with lead, zinc, and tungsten, as lesser resources. A zone of probable resource potential for talc, graphite, and marble is identified in the Mazourka Roadless Area. Metallic mineralization occurs mostly in vein deposits in silicic and carbonate metasedimentary rocks peripheral to Mesozoic plutons and locally in granitic rocks as well. There is little promise for the occurrence of fossil fuel resources in the roadless areas.

  3. Micropropagation and in vitro conservation of vanilla (Vanilla planifolia Andrews).

    PubMed

    Divakaran, Minoo; Babu, K Nirmal

    2009-01-01

    Vanilla (Vanilla planifolia Andrews (syn. V. fragrans Salisb.), a source of natural vanillin, plays a major positive role in the economy of several countries. A native to the Central America, its primary gene pool is threatened by deforestation and over collection that has resulted in disappearance of natural habitats and wild species. Therefore, multiplication and conservation of vanilla diversity is of paramount importance because of its narrow genetic base. It plays an important role in the production of disease free planting material for commercial cultivation. Simple protocols for micropropagation, in vitro conservation and synthetic seed production are described in this chapter which could further be applied to other related vanilla species as well.

  4. Prediction of posttraumatic stress symptoms in children after hurricane Andrew.

    PubMed

    Vernberg, E M; Silverman, W K; La Greca, A M; Prinstein, M J

    1996-05-01

    The authors used an integrative conceptual model to examine the emergence of posttraumatic stress disorder (PTSD) symptoms in 568 elementary school-age children 3 months after Hurricane Andrew. The model included 4 primary factors: Exposure to Traumatic Events, Child Characteristics, Access to Social Support, and Children's Coping. Overall, 62% of the variance in children's self-reported PTSD symptoms was accounted for by the 4 primary factors, and each factor improved overall prediction of symptoms when entered in the analyses in the order specified by the conceptual model. The findings suggest that the conceptual model may be helpful to organize research and intervention efforts in the wake of natural disasters.

  5. PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).

    PubMed

    Grundy, Gabrielle J; Polo, Luis M; Zeng, Zhihong; Rulten, Stuart L; Hoch, Nicolas C; Paomephan, Pathompong; Xu, Yingqi; Sweet, Steve M; Thorne, Alan W; Oliver, Antony W; Matthews, Steve J; Pearl, Laurence H; Caldecott, Keith W

    2016-01-01

    PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break. PMID:27530147

  6. Assistant DNA recycling with nicking endonuclease and molecular beacon for signal amplification using a target-complementary arched structure.

    PubMed

    Gao, Fenglei; Lei, Jianping; Ju, Huangxian

    2013-05-11

    A simple and universal method for ultrasensitive "signal on" detection of DNA was developed with a target-complementary arched structure to release assistant DNA, which was recycled with nicking endonuclease to amplify the detectable fluorescent signal of molecular beacons.

  7. Hurricane Andrew-related injuries and illnesses, Louisiana, 1992.

    PubMed

    McNabb, S J; Kelso, K Y; Wilson, S A; McFarland, L; Farley, T A

    1995-06-01

    To determine the extent and types of injuries and illnesses in Louisiana associated with or related to Hurricane Andrew, we gathered data from hospital emergency departments and coroner's offices on demographic variables, institution, nature and cause of the injury or illness, body part affected, location, and date and time of the event. A hurricane-related injury or illness was defined as one that occurred from noon on August 24, 1992, through midnight on September 21, 1992, as a direct or indirect result of the preparation for (preimpact), the impact of, or the clean-up after the hurricane (postimpact). Nineteen parishes in south-central Louisiana that were most affected by Hurricane Andrew provided data from patients seen in emergency departments and reports from coroner's offices. Active, advance surveillance of this type promotes and facilitates the reporting of disaster-related health outcomes. Future planning for hurricanes should take into account the high rate of cuts, lacerations, and puncture wounds, particularly during the postimpact phase.

  8. Pharmaceutical services at a medical site after Hurricane Andrew.

    PubMed

    Nestor, A; Aviles, A I; Kummerle, D R; Barclay, L P; Rey, J A

    1993-09-01

    The experiences of a group of volunteer clinical pharmacists who provided pharmacy services as part of a disaster relief effort following a hurricane are reported. Hurricane Andrew left many people in southern Florida without shelter and other basic necessities, including health care services. A group of seven pharmacists volunteered to provide services at a temporary medical site set up in a community center. The pharmacy stock consisted of donated drugs. The pharmacists dispensed medications directly to patients and worked closely with other volunteer medical personnel to make sure proper medications were used. Because the pharmacy stock was limited, physicians relied upon the pharmacists for information about therapeutic interchanges, dosage conversions, and new medications. Prescriptions were often ordered and dispensed with only oral instructions. The pharmacists also provided patient counseling, although problems caused by inexperience with certain types of patients, a language barrier, and substandard living conditions after the hurricane made counseling more difficult. The contributions of seven pharmacists who provided services at an emergency medical site after Hurricane Andrew were well received by other health care personnel and by the community.

  9. A Meta-Analytic Review of the MacAndrew Alcoholism Scale.

    ERIC Educational Resources Information Center

    Cooper-Hakim, Amy; Viswesvaran, Chockalingam

    2002-01-01

    Using meta analysis, examined the predictive validity of scores on the MacAndrew Alcoholism Scale (C. MacAndrew, 1965). Compared results for 161 studies with results for 63 studies using cut scores. Discusses why the use of continuous measures rather than cut scores is recommended. (SLD)

  10. Generating an Empirical Probability Distribution for the Andrews-Pregibon Statistic.

    ERIC Educational Resources Information Center

    Jarrell, Michele G.

    A probability distribution was developed for the Andrews-Pregibon (AP) statistic. The statistic, developed by D. F. Andrews and D. Pregibon (1978), identifies multivariate outliers. It is a ratio of the determinant of the data matrix with an observation deleted to the determinant of the entire data matrix. Although the AP statistic has been used…

  11. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The...

  12. Simpler May Still Be Better: A Reply to Eggerth and Andrew

    ERIC Educational Resources Information Center

    Gore, Paul A., Jr.; Brown, Steven D.

    2006-01-01

    Eggerth and Andrew (2006 [this issue]) provide a valuable contribution to researchers and practitioners using the C index to measure congruence when person or environment codes contain fewer than three letters. However, the computational procedures outlined by Eggerth and Andrew may be more complex than they need be. A simpler alternative to…

  13. Food-related coping strategies after Hurricane Andrew.

    PubMed

    Magnus, M H

    1994-06-01

    This telephone survey examined food-related coping strategies in Floridian households after Hurricane Andrew. Approximately 137 households of university faculty and staff who lived in hurricane-damaged areas were interviewed. The average respondent was a college-educated woman between 41 and 60 years old. Prevailing food-purchasing problems included food stores that were either closed, without perishable food, distant, or crowded. In the absence of electricity and water, changes in food preparation included preparation of meals without a stove, more frequent use of grills and canned food, simpler meals, and less cooking. Changes in kitchen cleanup included using more disposables, cleaning more often, washing dishes by hand, and cleaning up less often because of damage in the kitchen. Respondents indicated that the hurricane experience taught them that they should have acquired more general supplies (eg, coolers, thermoses, propane stoves, and gas burners), more water and ice, and more nonperishable foods before the hurricane.

  14. Measurement of perceived disruption during rebuilding following Hurricane Andrew.

    PubMed

    Burnett, K; Ironson, G; Benight, C; Wynings, C; Greenwood, D; Carver, C S; Cruess, D; Baum, A; Schneiderman, N

    1997-10-01

    The purpose of this study was to develop and evaluate a measure of perceived disruption during rebuilding following a disaster. Two eight-item scales, which measured intensity of disruption during the entire repair phase (Intensity-RP) and intensity of disruption during the past month (Intensity-PM) were developed and administered to 135 survivors of Hurricane Andrew. At 9 to 12 months postdisaster, Intensity-RP and Intensity-PM were both significantly associated with scores on the Global Severity Index of the SCL-90-R, and with scores on the Impact of Event-Intrusion Scale; Intensity-PM alone was significantly associated with PTSD scores. Regression analyses indicated that each scale contributed significant unique variance in predicting mental health symptoms, even after controlling for relevant demographic and initial disaster exposure variables.

  15. The McAndrews Leadership Lecture: February 2015, by Dr Scott Haldeman. Challenges of the Past, Challenges of the Present.

    PubMed

    Haldeman, Scott; McAndrews, George P; Goertz, Christine; Sportelli, Louis; Hamm, Anthony W; Johnson, Claire

    2015-12-01

    The McAndrews Leadership Lecture was developed by the American Chiropractic Association to honor the legacy of Jerome F. McAndrews, DC, and George P. McAndrews, JD, and their contributions to the chiropractic profession. This article is a transcription of the presentation made by Dr Scott Haldeman on February 28, 2015, in Washington, DC, at the National Chiropractic Leadership Conference.

  16. 76 FR 70433 - Andrew Peklo III; Notice of Application Accepted for Filing with the Commission, Intent to Waive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... Energy Regulatory Commission Andrew Peklo III; Notice of Application Accepted for Filing with the...: Exemption From Licensing. b. Project No.: 12790-001. c. Date filed: February 16, 2011. d. Applicant: Andrew.... Applicant Contact: Andrew Peklo III, 29 Pomperaug Road, Woodbury, CT 06798, (203) 263-4566,...

  17. 78 FR 21921 - Andrew Peklo III; Notice of Application Accepted for Filing, Intent To Waive Scoping, Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-12

    ... Energy Regulatory Commission Andrew Peklo III; Notice of Application Accepted for Filing, Intent To Waive... License. b. Project No.: 12790-002. c. Date filed: January 17, 2013. d. Applicant: Andrew Peklo III. e... Pursuant to: Federal Power Act (FPA) 16 U.S.C. 791 (a)- 825(r). h. Applicant Contact: Andrew Peklo III,...

  18. Real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons.

    PubMed

    Xu, Wentao; Wang, Chenguang; Zhu, Pengyu; Guo, Tianxiao; Xu, Yuancong; Huang, Kunlun; Luo, Yunbo

    2016-04-21

    Techniques of isothermal amplification have recently made great strides, and have generated significant interest in the field of point-of-care detection. Nicking endonuclease-mediated isothermal amplification (NEMA) is an example of simple isothermal technology. In this paper, a real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons (SMB-NEMA) of improved specificity and sensitivity is described. First, we optimized the prohibition of de novo synthesis by choosing Nt·BstNBI endonuclease. Second, the whole genome was successfully amplified with Nt·BstNBI (6 U), betaine (1 M) and trehalose (60 mM) for the first time. Third, we achieved 10 pg sensitivity for the first time after adding a small molecular beacon that spontaneously undergoes a conformational change when hybridizing to target, and the practical test validated the assay's application. The small molecular beacon has a similar melting temperature to the reaction temperature, but is approximately 10 bp shorter than the length of a traditional molecular beacon. A new threshold regulation was also established for isothermal conditions. Finally, we established a thermodynamic model for designing small molecular beacons. This multistate model is more correct than the traditional algorithm. This theoretical and practical basis will help us to monitor SMB-NEMA in a quantitative way. In summary, our SMB-NEMA method allows the simple, specific and sensitive assessment of isothermal DNA quantification. PMID:27027375

  19. Free energy analysis and mechanism of base pair stacking in nicked DNA

    PubMed Central

    Häse, Florian; Zacharias, Martin

    2016-01-01

    The equilibrium of stacked and unstacked base pairs is of central importance for all nucleic acid structure formation processes. The stacking equilibrium is influenced by intramolecular interactions between nucleosides but also by interactions with the solvent. Realistic simulations on nucleic acid structure formation and flexibility require an accurate description of the stacking geometry and stability and its sequence dependence. Free energy simulations have been conducted on a series of double stranded DNA molecules with a central strand break (nick) in one strand. The change in free energy upon unstacking was calculated for all ten possible base pair steps using umbrella sampling along a center-of-mass separation coordinate and including a comparison of different water models. Comparison to experimental studies indicates qualitative agreement of the stability order but a general overestimation of base pair stacking interactions in the simulations. A significant dependence of calculated nucleobase stacking free energies on the employed water model was observed with the tendency of stacking free energies being more accurately reproduced by more complex water models. The simulation studies also suggest a mechanism of stacking/unstacking that involves significant motions perpendicular to the reaction coordinate and indicate that the equilibrium nicked base pair step may slightly differ from regular B-DNA geometry in a sequence-dependent manner. PMID:27407106

  20. Free energy analysis and mechanism of base pair stacking in nicked DNA.

    PubMed

    Häse, Florian; Zacharias, Martin

    2016-09-01

    The equilibrium of stacked and unstacked base pairs is of central importance for all nucleic acid structure formation processes. The stacking equilibrium is influenced by intramolecular interactions between nucleosides but also by interactions with the solvent. Realistic simulations on nucleic acid structure formation and flexibility require an accurate description of the stacking geometry and stability and its sequence dependence. Free energy simulations have been conducted on a series of double stranded DNA molecules with a central strand break (nick) in one strand. The change in free energy upon unstacking was calculated for all ten possible base pair steps using umbrella sampling along a center-of-mass separation coordinate and including a comparison of different water models. Comparison to experimental studies indicates qualitative agreement of the stability order but a general overestimation of base pair stacking interactions in the simulations. A significant dependence of calculated nucleobase stacking free energies on the employed water model was observed with the tendency of stacking free energies being more accurately reproduced by more complex water models. The simulation studies also suggest a mechanism of stacking/unstacking that involves significant motions perpendicular to the reaction coordinate and indicate that the equilibrium nicked base pair step may slightly differ from regular B-DNA geometry in a sequence-dependent manner. PMID:27407106

  1. Real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons.

    PubMed

    Xu, Wentao; Wang, Chenguang; Zhu, Pengyu; Guo, Tianxiao; Xu, Yuancong; Huang, Kunlun; Luo, Yunbo

    2016-04-21

    Techniques of isothermal amplification have recently made great strides, and have generated significant interest in the field of point-of-care detection. Nicking endonuclease-mediated isothermal amplification (NEMA) is an example of simple isothermal technology. In this paper, a real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons (SMB-NEMA) of improved specificity and sensitivity is described. First, we optimized the prohibition of de novo synthesis by choosing Nt·BstNBI endonuclease. Second, the whole genome was successfully amplified with Nt·BstNBI (6 U), betaine (1 M) and trehalose (60 mM) for the first time. Third, we achieved 10 pg sensitivity for the first time after adding a small molecular beacon that spontaneously undergoes a conformational change when hybridizing to target, and the practical test validated the assay's application. The small molecular beacon has a similar melting temperature to the reaction temperature, but is approximately 10 bp shorter than the length of a traditional molecular beacon. A new threshold regulation was also established for isothermal conditions. Finally, we established a thermodynamic model for designing small molecular beacons. This multistate model is more correct than the traditional algorithm. This theoretical and practical basis will help us to monitor SMB-NEMA in a quantitative way. In summary, our SMB-NEMA method allows the simple, specific and sensitive assessment of isothermal DNA quantification.

  2. A transnational conference romance: Elsie Andrews, Hildegarde Kneeland, and the Pan-Pacific Women's Association.

    PubMed

    Laurie, Alison J

    2009-01-01

    Elsie Andrews, a feminist activist from New Zealand, met Dr. Hildegarde Kneeland, a progressive economist from the United States, at the 1934 Pan-Pacific Women's Association conference in Honolulu. Andrews wrote diaries of her attendance at conferences, and in these writes openly of her attraction and romantic feelings for Kneeland, despite her own long-term domestic partnership back in New Plymouth with Muriel Kirton. This article considers the role conference romances may have played for Andrews and others in encouraging their interest in women's organizations, in the context of literature on romantic friendships and lesbianism.

  3. A transnational conference romance: Elsie Andrews, Hildegarde Kneeland, and the Pan-Pacific Women's Association.

    PubMed

    Laurie, Alison J

    2009-01-01

    Elsie Andrews, a feminist activist from New Zealand, met Dr. Hildegarde Kneeland, a progressive economist from the United States, at the 1934 Pan-Pacific Women's Association conference in Honolulu. Andrews wrote diaries of her attendance at conferences, and in these writes openly of her attraction and romantic feelings for Kneeland, despite her own long-term domestic partnership back in New Plymouth with Muriel Kirton. This article considers the role conference romances may have played for Andrews and others in encouraging their interest in women's organizations, in the context of literature on romantic friendships and lesbianism. PMID:19830617

  4. Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA)

    PubMed Central

    Chauleau, Mathieu; Shuman, Stewart

    2016-01-01

    Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO4 nicks in duplex DNA via reaction of LigA with NAD+ to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO4 to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form a 3′-5′ phosphodiester (step 3). A distinctive feature of EcoLigA is its stimulation by ammonium ion. Here we used rapid mix-quench methods to analyze the kinetic mechanism of single-turnover nick sealing by EcoLigA–AMP. For substrates with correctly base-paired 3′-OH/5′-PO4 nicks, kstep2 was fast (6.8–27 s−1) and similar to kstep3 (8.3–42 s−1). Absent ammonium, kstep2 and kstep3 were 48-fold and 16-fold slower, respectively. EcoLigA was exquisitely sensitive to 3′-OH base mispairs and 3′ N:abasic lesions, which elicited 1000- to >20000-fold decrements in kstep2. The exception was the non-canonical 3′ A:oxoG configuration, which EcoLigA accepted as correctly paired for rapid sealing. These results underscore: (i) how EcoLigA requires proper positioning of the nick 3′ nucleoside for catalysis of 5′ adenylylation; and (ii) EcoLigA's potential to embed mutations during the repair of oxidative damage. EcoLigA was relatively tolerant of 5′-phosphate base mispairs and 5′ N:abasic lesions. PMID:26857547

  5. RNA nicking activity associated with DNA ligase of T4 infected E. coli: properties and influence on in vitro reactions of ligase.

    PubMed Central

    Sano, H; Feix, G

    1975-01-01

    Highly purified DNA ligase from T4 infected E. coli displays an RNA nicking activity which cleaves endonucleolytically the RNA of ribo-desoxy-and ribo-ribo type doublestranded structures to oligonucleotides with 5'phosphoryl-and 3'hydroxy termini. In the presence of ATP the generated nicks are repaired by the ligase except at the ends of the doublestranded regions where some short oligonucleotides are released before ligation can occur. As judged from its behaviour during the various purification steps and from some of its properties, the nicking activity seems to be different from known nicking enzymes. PMID:1101228

  6. Lessons from isolable nickel(I) precursor complexes for small molecule activation.

    PubMed

    Yao, Shenglai; Driess, Matthias

    2012-02-21

    Small-molecule activation by transition metals is essential to numerous organic transformations, both biological and industrial. Creating useful metal-mediated activation systems often depends on stabilizing the metal with uncommon low oxidation states and low coordination numbers. This provides a redox-active metal center with vacant coordination sites well suited for interacting with small molecules. Monovalent nickel species, with their d(9) electronic configuration, are moderately strong one-electron reducing agents that are synthetically attractive if they can be isolated. They represent suitable reagents for closing the knowledge gap in nickel-mediated activation of small molecules. Recently, the first strikingly stable dinuclear β-diketiminate nickel(I) precursor complexes were synthesized, proving to be suitable promoters for small-molecule binding and activation. They have led to many unprecedented nickel complexes bearing activated small molecules in different reduction stages. In this Account, we describe selected achievements in the activation of nitrous oxide (N(2)O), O(2), the heavier chalcogens (S, Se, and Te), and white phosphorus (P(4)) through this β-diketiminatonickel(I) precursor species. We emphasize the reductive activation of O(2), owing to its promise in oxidation processes. The one-electron-reduced O(2) activation product, that is, the corresponding β-diketiminato-supported Ni-O(2) complex, is a genuine superoxonickel(II) complex, representing an important intermediate in the early stages of O(2) activation. It selectively acts as an oxygen-atom transfer agent, hydrogen-atom scavenger, or both towards exogenous organic substrates to yield oxidation products. The one-electron reduction of the superoxonickel(II) moiety was examined by using elemental potassium, β-diketiminatozinc(II) chloride, and β-diketiminatoiron(I) complexes, affording the first heterobimetallic complexes featuring a [NiO(2)M] subunit (M is K, Zn, or Fe). Through

  7. AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism.

    PubMed Central

    Montecucco, A; Ciarrocchi, G

    1988-01-01

    In the presence of AMP and Mg2+, a covalently closed duplex DNA containing negative superhelical turns was treated with DNA ligase isolated from bacteriophage T4-infected E. coli. This resulted in the gradual and not sudden loss of superhelical turns as for example in the case of type I DNA topoisomerase. All DNA products remain covalently closed. Since T4 enzyme-mediated DNA relaxation is inhibited by both pyrophosphate and by ATP this suggests that DNA relaxing and DNA joining activities probably coincide. EDTA addition in the presence of a large excess of enzyme, induces the formation of nicked DNA products while protein denaturing treatments are not very effective. Our observations might suggest an involvement of the relaxing activity of DNA ligase during the ligation process. Images PMID:3137526

  8. Isothermal amplification detection of nucleic acids by a double-nicked beacon.

    PubMed

    Shi, Chao; Zhou, Meiling; Pan, Mei; Zhong, Guilin; Ma, Cuiping

    2016-03-01

    Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use.

  9. Isothermal amplification detection of nucleic acids by a double-nicked beacon.

    PubMed

    Shi, Chao; Zhou, Meiling; Pan, Mei; Zhong, Guilin; Ma, Cuiping

    2016-03-01

    Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use. PMID:26706801

  10. ISS Update: RATS Principal Investigator Andrew Abercromby -- 08.29.12

    NASA Video Gallery

    NASA Public Affairs Officer Brandi Dean talks to the Research And Technology Studies (RATS) Principal Investigator Andrew Abercromby in the Space Vehicle Mock-Up Facility at Johnson Space Center in...

  11. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... waterline to 30°09′57.5″ N, 085°44′37″ W; then northerly to point of origin. (2) Area BA-1. The area...

  12. Andrew meets Rensch: sexual size dimorphism and the inverse of Rensch's rule in Andrew's toad (Bufo andrewsi).

    PubMed

    Liao, Wen Bo; Liu, Wen Chao; Merilä, Juha

    2015-02-01

    Variation in sexual size dimorphism (SSD) is a widespread phenomenon and is commonly attributed to variation in sex-specific patterns of selection. According to Rensch's rule, SSD increases with increasing body size when males are the larger sex, and decreases when females are the larger sex. Using data from 17 populations of Andrew's toad (Bufo andrewsi), we tested whether the patterns of SSD conform to Rensch's rule. Using field experiments, we also evaluated the hypothesis that sexual selection favours large male body size and that fecundity selection favours large female body size. The results revealed that the degree of SSD increased with increasing mean size in females, consistent with the inverse of Rensch's rule. Although experiments revealed evidence for a large-male mating advantage, selection for large male size was weak at best, and hence unlikely to be an important source of variation in SSD. However, fecundity selection favouring large females was evident, and likely to explain the observed inverse of Rensch's rule. After correcting male and female body size for age differences, the patterns of SSD remained the same, suggesting that the intra- and interpopulational variation in SSD is not driven by sex differences in age structure. Hence, these findings suggest that the strong fecundity selection favouring large females drives the evolution of female-biased SSD in B. andrewsi, providing an explanation for the inverse of Rensch's rule. As such, the study provides an important addition to the small body of literature that uses an intraspecific approach to demonstrate the inverse of Rensch's rule.

  13. The McAndrews Leadership Lecture: February 2015, by Dr Scott Haldeman. Challenges of the Past, Challenges of the Present

    PubMed Central

    Haldeman, Scott; McAndrews, George P.; Goertz, Christine; Sportelli, Louis; Hamm, Anthony W.; Johnson, Claire

    2015-01-01

    The McAndrews Leadership Lecture was developed by the American Chiropractic Association to honor the legacy of Jerome F. McAndrews, DC, and George P. McAndrews, JD, and their contributions to the chiropractic profession. This article is a transcription of the presentation made by Dr Scott Haldeman on February 28, 2015, in Washington, DC, at the National Chiropractic Leadership Conference. PMID:26770177

  14. Hurricane Andrew's impact on natural gas and oil facilities on the outer continental shelf (interim report as of November 1993)

    SciTech Connect

    Daniels, G.R.

    1994-01-01

    The interim report reviews Hurricane Andrew's impact on Federal Outer Continental Shelf (OCS) natural gas and oil drilling and production facilities. The report provides background on Hurricane Andrew's progression, discusses how OCS operators responded to the storm, summarizes the types of damage to offshore facilies caused by Hurricane Andrew, and discusses Minerals Management Service's continuing damage assessment and repair efforts. The summaries of damage estimates are presented in tables in Appendix 1. A glossary of report terminology is provided in Appendix 2.

  15. Standard and AEGIS nicking molecular beacons detect amplicons from the Middle East respiratory syndrome coronavirus.

    PubMed

    Yaren, Ozlem; Glushakova, Lyudmyla G; Bradley, Kevin M; Hoshika, Shuichi; Benner, Steven A

    2016-10-01

    This paper combines two advances to detect MERS-CoV, the causative agent of Middle East Respiratory Syndrome, that have emerged over the past few years from the new field of "synthetic biology". Both are based on an older concept, where molecular beacons are used as the downstream detection of viral RNA in biological mixtures followed by reverse transcription PCR amplification. The first advance exploits the artificially expanded genetic information systems (AEGIS). AEGIS adds nucleotides to the four found in standard DNA and RNA (xNA); AEGIS nucleotides pair orthogonally to the A:T and G:C pairs. Placing AEGIS components in the stems of molecular beacons is shown to lower noise by preventing unwanted stem invasion by adventitious natural xNA. This should improve the signal-to-noise ratio of molecular beacons operating in complex biological mixtures. The second advance introduces a nicking enzyme that allows a single target molecule to activate more than one beacon, allowing "signal amplification". Combining these technologies in primers with components of a self-avoiding molecular recognition system (SAMRS), we detect 50 copies of MERS-CoV RNA in a multiplexed respiratory virus panel by generating fluorescence signal visible to human eye and/or camera. PMID:27421627

  16. PepFects and NickFects for the Intracellular Delivery of Nucleic Acids.

    PubMed

    Arukuusk, Piret; Pärnaste, Ly; Hällbrink, Mattias; Langel, Ülo

    2015-01-01

    Nucleic acids can be utilized in gene therapy to restore, alter, or silence gene functions. In order to reveal the biological activity nucleic acids have to reach their intracellular targets by passing through the plasma membrane, which is impermeable for these large and negatively charged molecules. Cell-penetrating peptides (CPPs) condense nucleic acids into nanoparticles using non-covalent complexation strategy and mediate their delivery into the cell, whereas the physicochemical parameters of the nanoparticles determine the interactions with the membranes, uptake mechanism, and subsequent intracellular fate. The nanoparticles are mostly internalized by endocytosis that leads to the entrapment of them in endosomal vesicles. Therefore design of new CPPs that are applicable for non-covalent complex formation strategy and harness endosomolytic properties is highly vital. Here we demonstrate that PepFects and NickFects are efficient vectors for the intracellular delivery of various nucleic acids.This chapter describes how to form CPP/pDNA nanoparticles, evaluate stable nanoparticles formation, and assess gene delivery efficacy.

  17. Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase

    PubMed Central

    Unciuleac, Mihaela-Carmen; Goldgur, Yehuda; Shuman, Stewart

    2015-01-01

    ATP-dependent RNA ligases are agents of RNA repair that join 3′-OH and 5′-PO4 RNA ends. Naegleria gruberi RNA ligase (NgrRnl) exemplifies a family of RNA nick-sealing enzymes found in bacteria, viruses, and eukarya. Crystal structures of NgrRnl at three discrete steps along the reaction pathway—covalent ligase-(lysyl-Nζ)–AMP•Mn2+ intermediate; ligase•ATP•(Mn2+)2 Michaelis complex; and ligase•Mn2+ complex—highlight a two-metal mechanism of nucleotidyl transfer, whereby (i) an enzyme-bound “catalytic” metal coordination complex lowers the pKa of the lysine nucleophile and stabilizes the transition state of the ATP α phosphate; and (ii) a second metal coordination complex bridges the β- and γ-phosphates. The NgrRnl N domain is a distinctively embellished oligonucleotide-binding (OB) fold that engages the γ-phosphate and associated metal complex and orients the pyrophosphate leaving group for in-line catalysis with stereochemical inversion at the AMP phosphate. The unique domain architecture of NgrRnl fortifies the theme that RNA ligases have evolved many times, and independently, by fusions of a shared nucleotidyltransferase domain to structurally diverse flanking modules. The mechanistic insights to lysine adenylylation gained from the NgrRnl structures are likely to apply broadly to the covalent nucleotidyltransferase superfamily of RNA ligases, DNA ligases, and RNA capping enzymes. PMID:26512110

  18. Standard and AEGIS nicking molecular beacons detect amplicons from the Middle East respiratory syndrome coronavirus.

    PubMed

    Yaren, Ozlem; Glushakova, Lyudmyla G; Bradley, Kevin M; Hoshika, Shuichi; Benner, Steven A

    2016-10-01

    This paper combines two advances to detect MERS-CoV, the causative agent of Middle East Respiratory Syndrome, that have emerged over the past few years from the new field of "synthetic biology". Both are based on an older concept, where molecular beacons are used as the downstream detection of viral RNA in biological mixtures followed by reverse transcription PCR amplification. The first advance exploits the artificially expanded genetic information systems (AEGIS). AEGIS adds nucleotides to the four found in standard DNA and RNA (xNA); AEGIS nucleotides pair orthogonally to the A:T and G:C pairs. Placing AEGIS components in the stems of molecular beacons is shown to lower noise by preventing unwanted stem invasion by adventitious natural xNA. This should improve the signal-to-noise ratio of molecular beacons operating in complex biological mixtures. The second advance introduces a nicking enzyme that allows a single target molecule to activate more than one beacon, allowing "signal amplification". Combining these technologies in primers with components of a self-avoiding molecular recognition system (SAMRS), we detect 50 copies of MERS-CoV RNA in a multiplexed respiratory virus panel by generating fluorescence signal visible to human eye and/or camera.

  19. Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI.

    PubMed

    Xu, Shuang-Yong; Zhu, Zhenyu; Zhang, Penghua; Chan, Siu-Hong; Samuelson, James C; Xiao, Jianping; Ingalls, Debra; Wilson, Geoffrey G

    2007-01-01

    BsrDI and BtsI restriction endonucleases recognize and cleave double-strand DNA at the sequences GCAATG (2/0) and GCAGTG (2/0), respectively. We have purified and partially characterized these two enzymes, and analyzed the genes that encode them. BsrDI and BtsI are unusual in two respects: each cleaves DNA as a heterodimer of one large subunit (B subunit) and one small subunit (A subunit); and, in the absence of their small subunits, the large subunits behave as sequence-specific DNA nicking enzymes and only nick the bottom strand of the sequences at these respective positions: GCAATG (-/0) and GCAGTG (-/0). We refer to the single subunit, the bottom-strand nicking forms as 'hemidimers'. Amino acid sequence comparisons reveal that BsrDI and BtsI belong to a family of restriction enzymes that possess two catalytic sites: a canonical PD-X(n)-EXK and a second non-canonical PD-X(n)-E-X12-QR. Interestingly, the other family members, which include BsrI (ACTGG 1/-1) and BsmI/Mva1269I (GAATGC 1/-1) are single polypeptide chains, i.e. monomers, rather than heterodimers. In BsrDI and BtsI, the two catalytic sites are found in two separate subunits. Site-directed mutagenesis confirmed that the canonical catalytic site located at the N-terminus of the large subunit is responsible for the bottom-strand cleavage, whereas the non-canonical catalytic site located in the small subunit is responsible for hydrolysis of the top strand. Top-strand specific nicking variants, Nt.BsrDI and Nt.BtsI, were successfully engineered by combining the catalytic-deficient B subunit with wild-type A subunit. PMID:17586812

  20. Preliminary report: medical examiner reports of deaths associated with Hurricane Andrew--Florida, August 1992.

    PubMed

    1992-09-01

    On August 24, 1992, at 1:40 a.m. eastern daylight time (EDT), rain bands associated with Hurricane Andrew reached the eastern coast of Florida. At 4:45 a.m. EDT, Hurricane Andrew made landfall 35 miles southeast of Miami at Homestead, with sustained winds of 145 miles per hour (mph) and gusts of 164 mph. These winds extended 45 miles outward of the storm center. The storm moved across the state at 18 mph toward the Gulf of Mexico (Figure 1). The tidal surge on the eastern coast was estimated at 7-19 feet. During the storm, approximately 2.5 million Florida residents were left without electrical power, and approximately 56,000 family dwelling units were destroyed or severely damaged. This report presents preliminary data from Florida medical examiner (ME) offices about deaths attributed to Hurricane Andrew.

  1. Andrew Sexton Gray (1826-1907). A founder of Australian ophthalmology: his life and times.

    PubMed

    Lowe, R F

    1985-11-01

    Andrew Sexton Gray was born in Limerick, Ireland, medically trained in Dublin, and was assistant to William Wilde, the distinguished oculist and aurist. He migrated to Victoria in 1859, was surgeon to a railway's construction company, then in 1862 began practice as a surgeon and oculist in Melbourne. In 1863 he founded a charitable eye and ear hospital, and had a very active, long life devoted mostly to ophthalmology. The hospital progressively expanded and became the centre for training for many ophthalmologists, as well as the nucleus for the cohesion of Victorian ophthalmology. History shows Andrew Sexton Gray to have been a founder of Australian ophthalmology. PMID:3914312

  2. Analyzing after-action reports from Hurricanes Andrew and Katrina: repeated, modified, and newly created recommendations.

    PubMed

    Knox, Claire Connolly

    2013-01-01

    Thirteen years after Hurricane Andrew struck Homestead, FL, Hurricane Katrina devastated the Gulf Coast of Mississippi, Alabama, and southeastern Louisiana. Along with all its destruction, the term "catastrophic" was redefined. This article extends the literature on these hurricanes by providing a macrolevel analysis of The Governor's Disaster Planning and Response Review Committee Final Report from Hurricane Andrew and three federal after-action reports from Hurricane Katrina, as well as a cursory review of relevant literature. Results provide evidence that previous lessons have not been learned or institutionalized with many recommendations being repeated or modified. This article concludes with a discussion of these lessons, as well as new issues arising during Hurricane Katrina.

  3. Andrew's bridge system: an aesthetic and functional option for rehabilitation of compromised maxillary anterior dentition

    PubMed Central

    Tambe, Abhijit; Patil, Sanjayagouda B; Bhat, Sudhakara; Badadare, Mokshada M

    2014-01-01

    Summary A patient with several missing teeth in the anterior aesthetic region along with severe ridge defect poses a greater challenge for prosthodontic rehabilitation. In such cases treatment using fixed partial denture (FPD) may not be feasible because of the extent of edentulous span and the periodontal conditions of the abutment teeth. To present a case of multiple missing maxillary anterior teeth with class III ridge defect rehabilitated using FPD-removable partial denture. A 38-year-old female patient was successfully rehabilitated using Andrew's bridge system in the maxillary anterior region. The fixed-removable Andrew's bridge system provides a good prognosis if diagnosed and planned meticulously. PMID:25035444

  4. Capacities of template-type platforms in the Gulf of Mexico during hurricane Andrew

    SciTech Connect

    Bea, R.G.; Loch, K.J.; Young, P.L.

    1997-02-01

    This paper details results from nonlinear analyses of the ultimate limit state performance characteristics of four Gulf of Mexico (GOM) platforms subjected to intense loadings from hurricane Andrew. These four platforms were located to the east of the track of hurricane Andrew, and were thus in the most intense portion of the storm (Smith, 1993). The nonlinear analyses are able to replicate details of the observed behavior of the four structures. This replication is very dependent on realistic characterization of the performance characteristics of the pile foundations and on accurate information on the as is condition of the platforms before the storm.

  5. Optimization of a DNA nicking assay to evaluate Oenocarpus bataua and Camellia sinensis antioxidant capacity.

    PubMed

    Leba, Louis-Jérôme; Brunschwig, Christel; Saout, Mona; Martial, Karine; Vulcain, Emmanuelle; Bereau, Didier; Robinson, Jean-Charles

    2014-10-09

    This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. Special attention has been paid to removing the protective and damaging effect of the solvent and FeSO4 respectively, as well as to identifying the relevant positive and negative controls. For each solvent, the optimal conditions were determined: (i) for aqueous extracts, 0.33 mM of FeSO4 and 0.62 mM of EDTA were incubated for 20 min at 37 °C; (ii) for acetone extracts, 1.16% solvent were incubated for 15 min at 37 °C with 1.3 mM of FeSO4 and 2.5 mM of EDTA and (iii) for methanol extracts, 0.16% solvent, were incubated for 1.5 h at 37 °C with 0.33 mM of FeSO4 and 0.62 mM of EDTA. Using the optimized conditions, the DNA damage protective activity of aqueous, methanolic and acetonic extracts of an Amazonian palm berry (Oenocarpus bataua) and green tea (Camellia sinensis) was assessed. Aqueous and acetonic Oenocarpus bataua extracts were protective against DNA damage, whereas aqueous, methanolic and acetonic extracts of Camellia sinensis extracts induced DNA damage.

  6. A robust probe for lighting up intracellular telomerase via primer extension to open a nicked molecular beacon.

    PubMed

    Qian, Ruocan; Ding, Lin; Yan, Liwen; Lin, Manfei; Ju, Huangxian

    2014-06-11

    A nicked molecular beacon (MB)-functionalized probe has been designed for in situ imaging and detection of intracellular telomerase activity. The nick separates the MB into two segments: a shorter telomerase primer (TSP) sequence as a part of the 5'-end stem and a longer sequence to form a loop with one thiol-labeled 3'-end stem. The MB can be opened by substitutional hybridization of the telomerase-triggered stem elongation product, which leads to separation of the Cy5 at the 5'-end nick from the gold nanoparticle (AuNP) as the nanocarrier and thus inhibits the energy transfer from Cy5 to AuNP. Upon endocytosis of the probe, the TSP can be extended by intracellular telomerase at its 3' end to produce the telomeric repeated sequence, which leads to the inner chain substitution and thus turns on the fluorescence of Cy5. The probe provides a one-step incubation technique for quantification and monitoring of the telomerase activity in living cells. The practicality of the proposed approach for distinguishing tumor cells from normal cells and monitoring the decrease of telomerase activity during treatment with antitumor drugs demonstrates its potential in clinical diagnostic and therapeutic monitoring.

  7. The Concurrent and Construct Validity of the MacAndrew Alcoholism Scale among At-Risk Adolescent Males.

    ERIC Educational Resources Information Center

    Moore, Richard H.

    1984-01-01

    Investigated the empirical validity of the MacAndrew Alcoholism Scale in adolescent male offenders (N=160). Results supported the concurrent validity of the MacAndrew Scale as a measure of alcohol abuse among young at-risk males and was also sensitive to polydrug use among youth who abuse alcohol. (LLL)

  8. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters...

  9. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters...

  10. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters...

  11. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters...

  12. Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-77 ESC VIEW --- Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while in the mid-deck of the Earth-orbiting Space Shuttle Endeavour. The scene was recorded with an Electronic Still Camera (ESC).

  13. 75 FR 41895 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers From Andrews...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... Workers From Andrews International, Inc., M.H. Equipment, and Kenco Logistics Services, LLC, Evansville... notice was published in the Federal Register on March 5, 2010 (75 FR 10321). The notice was amended on... published on the Federal Register on June 7, 2010 (75 FR 32221). At the request of the petitioners,...

  14. 77 FR 2968 - Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply Comments On December 15, 2011, the Commission issued notice that Office of Energy Projects staff will hold a site visit...

  15. Athena, Andrew and Stanford: A Look at Implementation and Evaluation in Three Large Projects.

    ERIC Educational Resources Information Center

    Isaacs, Geoff

    1989-01-01

    Describes implementation, support, and evaluation of computer assisted learning (CAL) projects at three universities: Project Athena at Massachusetts Institute of Technology; the Andrew network at Carnegie Mellon University; and a project at Stanford University. Topics discussed include work stations, microcomputers, computer networks, graphics,…

  16. 78 FR 78349 - Andrew Peklo III; Notice of Availability of Draft Environmental Assessment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... Commission's (Commission) regulations, 18 CFR Part 380 (Order No. 486, 52 FR 47897), the Office of Energy... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Andrew Peklo III; Notice of Availability of Draft Environmental...

  17. Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-75 ONBOARD VIEW --- Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the flight deck of the Space Shuttle Columbia. Allen was joined by four other astronauts and an international payload specialist for more than 16 days of research aboard Columbia. The photograph was taken with a 70mm handheld camera.

  18. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Naval Support Activity Panama... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  19. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Naval Support Activity Panama... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  20. 75 FR 10457 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... Forest Service Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration... statement. SUMMARY: The AP Loblolly Pine Removal and Restoration Project is a district-wide project that... endangered smooth coneflower. The AP Loblolly Pine Removal and Restoration Project is located on...

  1. Because Everyone Has a Story to Tell: Interview with Andrew Wright

    ERIC Educational Resources Information Center

    Floris, Flora Debora

    2016-01-01

    This article presents an interview with Andrew Wright, a widely recognized author, illustrator, storyteller, and teacher trainer. Wright has published many ELT books, authored six "Spellbinder" graded readers (1992-1994), and a collection of short stories. As a teacher trainer, Wright worked extensively with both teachers and students in…

  2. Amos Kendall's Role in the Election of Andrew Jackson as President of the United States, 1828.

    ERIC Educational Resources Information Center

    Nowell, Bob

    Amos Kendall's place in journalism history rests largely on his service as a journalist turned government official in the two administrations of President Andrew Jackson. Historians have claimed that Kendall was an influential journalist of the "partisan press" era, but they have provided little documentation. That documentation has been provided…

  3. 14. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Andrew McLean Company Textile Mill looking north; Building No. 6 at right, Building No. 1 in background, gateposts in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  4. 22. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Andrew McLean Company Textile Mill looking north; Building No. 1 at left, Building No. 4 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  5. 16. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. View of Andrew McLean Company Textile Mill looking southeast; Building No. 6 at center, roof of Building No. 1 at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  6. 18. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Andrew McLean Company Textile Mill looking north; Building No. 3 at center right, Building No. 2 at center distance, Dundee Canal at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  7. 20. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Andrew McLean Company Textile Mill looking southeast; Building No. 2 at left, Building No. 3 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  8. 76 FR 79675 - Pomperaug Hydro Project Andrew Peklo III; Notice of Site Visit and Technical Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project Andrew Peklo III; Notice of Site Visit and Technical Meeting On January 18, 2012, Office of Energy Projects staff will hold a site visit and technical...

  9. Commentary on Andrew Coulson's "Comparing Public, Private, and Market Schools: The International Evidence"

    ERIC Educational Resources Information Center

    Wolfram, Gary; Coulson, Andrew J.

    2009-01-01

    In this article, the authors comment on Andrew Coulson's "Comparing Public, Private, and Market Schools: The International Evidence." The authors believe that Coulson's paper is a very interesting review of the literature on the ability of market-produced education to outperform government-produced education. Coulson's response on this commentary…

  10. 17. View looking north; Andrew McLean Company Textile Mill, Building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. View looking north; Andrew McLean Company Textile Mill, Building No. 1, at right, Botany Worsted Mills at left distance - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  11. 19. View of Andrew McLean Company Textile Mill looking south; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Andrew McLean Company Textile Mill looking south; Building No. 2 at center, Botany Mills Lanolin Retrieval plant site in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  12. 56. Aerial view looking west; Andrew McLean Company Textile Mill ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. Aerial view looking west; Andrew McLean Company Textile Mill at bottom, Dundee Canal at center, Botany Worsted Mills at top - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  13. 23. View of open firststory passageway of Andrew McLean Company ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of open first-story passageway of Andrew McLean Company Textile Mill Building No. 1, looking west - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  14. 15. View of Andrew McLean Company Textile Mill looking northeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. View of Andrew McLean Company Textile Mill looking northeast; Building No. 1 at left, Building No. 6 at center - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  15. 31. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  16. 30. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  17. 77 FR 20621 - Pomperaug Hydro Project; Andrew Peklo III; Notice Extending Deadline for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project; Andrew Peklo III; Notice Extending Deadline for Comments On January 18, 2012, Office of Energy Projects staff held a technical meeting in Woodbury, CT....

  18. News of Hurricane Andrew: The Agenda of Sources and the Sources' Agendas.

    ERIC Educational Resources Information Center

    Salwen, Michael B.

    1995-01-01

    Studies quotations in newspaper coverage of Hurricane Andrew, showing that individuals who were not affiliated with government or business were quoted most often. Shows that most sources were quoted as experts, with individuals represented as suffering victims, providing the news media with human interest quotations. Notes that most sources…

  19. Posttraumatic Stress, Depression, and Social Support among College Students after Hurricane Andrew.

    ERIC Educational Resources Information Center

    Pickens, Jeffrey; And Others

    1995-01-01

    One month after Hurricane Andrew, surveyed students who reported experiencing the most severe impact damage from the storm also reported experiencing the most stress, anxiety, and depressive symptoms. Regression analyses revealed that material and emotional social support were significant predictors of anxiety and depression scores after the…

  20. Stressing Memory: Long-Term Relations among Children's Stress, Recall and Psychological Outcome following Hurricane Andrew

    ERIC Educational Resources Information Center

    Sales, Jessica McDermott; Fivush, Robyn; Parker, Janat; Bahrick, Lorraine

    2005-01-01

    We examined relations among stress, children's recall, and psychological functioning following Hurricane Andrew. Thirty-five children from mixed socioeconomic backgrounds were divided into low-, moderate-, and high-stress groups and were interviewed about the hurricane immediately after the storm and 6 years later. Our primary interest, stemming…

  1. Crisis Intervention with Survivors of Natural Disaster: Lessons from Hurricane Andrew.

    ERIC Educational Resources Information Center

    Shelby, Janine S.; Tredinnick, Michael G.

    1995-01-01

    Crisis intervention has typically been conceptualized as seeking a return of clients to a state of equilibrium. Personal work experience with Hurricane Andrew survivors has led to an appreciation of the importance of several considerations. Develops a proactive approach, attempting to recognize and extend clients' preexisting strengths. Offers…

  2. Traversing the Gap: Andrew Wright, John Hick and Critical Religious Education

    ERIC Educational Resources Information Center

    Teece, Geoff

    2005-01-01

    This paper discusses aspects of Andrew Wright's version of a liberal, critical religious education and his criticisms of some other views of modern religious education. This is attempted not by examining these "other views" as such but by concentrating on the work of John Hick. The reason for this is that Wright, like Cooling (in his book "A…

  3. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  4. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  5. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  6. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Andrew Bay East Entrance, small-arms firing range, Tyndall Air Force Base, Fla. 334.680 Section 334.680... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  7. A murine host cell factor required for nicking of the dimer bridge of MVM recognizes two CG nucleotides displaced by 10 basepairs.

    PubMed

    Liu, Q; Astell, C R

    1996-10-01

    During replication of the minute virus of mice (MVM) genome, a dimer replicative form (RF) intermediate is resolved into two monomer RF molecules in such a way as to retain a unique sequence within the left hand hairpin terminus of the viral genome. Although the proposed mechanism for resolution of the dimer RF remains uncertain, it likely involves site-specific nicking of the dimer bridge. The RF contains two double-stranded copies of the viral genome joined by the extended 3' hairpin. Minor sequence asymmetries within the 3' hairpin allow the two halves of the dimer bridge to be distinguished. The A half contains the sequence [sequence: see text], whereas the B half contains the sequence [sequence: see text]. Using an in vitro assay, we show that only the B half of the MVM dimer bridge is nicked site-specifically when incubated with crude NS-1 protein (expressed in insect cells) and mouse LA9 cellular extract. When highly purified NS-1, the major nonstructural protein of MVM, is used in this nicking reaction, there is an absolute requirement for the LA9 cellular extract, suggesting a cellular factor (or factors) is (are) required. A series of mutations were created in the putative host factor binding region (HFBR) on the B half of the MVM dimer bridge adjacent to the NS-1 binding site. Nicking assays of these B half mutants showed that two CG motifs displaced by 10 nucleotides are important for nicking. Gel mobility shift assays demonstrated that a host factor(s) can bind to the HFBR of the B half of the dimer bridge and efficient binding depends on the presence of both CG motifs. Competitor DNA containing the wild-type HFBR sequence is able to specifically inhibit nicking of the B half, indicating that the host factor(s) bound to the HFBR is(are) essential for site-specific nicking to occur.

  8. Activation of Aryl Halides by Nickel(I) Pincer Complexes: Reaction Pathways of Stoichiometric and Catalytic Dehalogenations.

    PubMed

    Rettenmeier, Christoph A; Wenz, Jan; Wadepohl, Hubert; Gade, Lutz H

    2016-08-15

    Homolytic C-X bond cleavage of organohalides by the T-shaped nickel(I) complexes [LigNi(I)] 1 bearing the iso-PyrrMeBox ligand had been found previously to be the crucial activation step in the asymmetric hydrodehalogenation of geminal dihalides. Here, this mechanistic investigation is extended to aryl halides, which allowed a systematic study of the activation process by a combination of experimental data and density functional theory modeling. While the activation of both aryl chlorides and geminal dichlorides appears to proceed via an analogous transition state, the generation of a highly stabile nickel(II)aryl species in the reaction of the aryl chlorides for the former represents a major difference in the reactive behavior. This difference was found to have a crucial impact on the activity of these nickel pincer systems as catalysts in the dehalogenation of aryl chlorides compared to geminal dichlorides and highlights the importance of the regulatory pathways controlling the nickel(I) concentration throughout the catalysis. These results along with the identification and characterization of novel nickel(II)aryl species are presented. PMID:27483018

  9. Analyzing after-action reports from Hurricanes Andrew and Katrina: repeated, modified, and newly created recommendations.

    PubMed

    Knox, Claire Connolly

    2013-01-01

    Thirteen years after Hurricane Andrew struck Homestead, FL, Hurricane Katrina devastated the Gulf Coast of Mississippi, Alabama, and southeastern Louisiana. Along with all its destruction, the term "catastrophic" was redefined. This article extends the literature on these hurricanes by providing a macrolevel analysis of The Governor's Disaster Planning and Response Review Committee Final Report from Hurricane Andrew and three federal after-action reports from Hurricane Katrina, as well as a cursory review of relevant literature. Results provide evidence that previous lessons have not been learned or institutionalized with many recommendations being repeated or modified. This article concludes with a discussion of these lessons, as well as new issues arising during Hurricane Katrina. PMID:24180096

  10. Impact of an extreme event on the sediment budget: Hurricane Andrew in the Louisiana barrier islands

    USGS Publications Warehouse

    List, Jeffrey H.; Hansen, Mark E.; Sallenger,, Asbury H.; Jaffe, Bruce E.; Edge, B.L

    1997-01-01

    This paper examines the influence of Hurricane Andrew on the sediment budget of an 80-kilometer section of the Louisiana barrier islands west of the modern Mississippi delta. Because long-term bathymetric change has been extensively studied in this area, excellent baseline data are available for evaluating the impact of Hurricane Andrew. Results show that despite the high intensity of the storm and a storm track optimally positioned to impact the study area, the storm did not have an overwhelming influence on the sediment budget when compared to the changes occurring over the previous 50 years. For the Louisiana barrier islands, a 50-year record appears to be adequate for averaging the long-term contributions of both major and minor storm events to the sediment budget.

  11. Joint Task Force Andrew: the 44th Medical Brigade mental health staff officer's after action review.

    PubMed

    Holsenbeck, L S

    1994-03-01

    The massive Department of Defense deployment in support of Hurricane Andrew relief cast the military medical departments in a new role. Military medical personnel were challenged to apply the traditional principles of combat medicine to a noncombat environment, within the continental United States, within an existing health care infrastructure, in a role subordinate to local civilian health care agencies. As a medical "subject matter expert" assigned to the Joint Task Force Andrew Surgeon's staff, the author worked at the civil-military interface. The lessons learned in his role as a special staff officer should benefit any health care provider involved in disaster relief. They focus on problem areas peculiar to the disaster relief scenario.

  12. 78 FR 6173 - Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF TRANSPORTATION Surface Transportation Board Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen... Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and Richard Kosiba...

  13. In celebration of the 60th birthday of Professor Andrew D. Hamilton FRS.

    PubMed

    Thompson, Sam; Wilson, Andrew J; Battersby, Alan R

    2013-10-01

    An on-line collection of articles celebrating the 60th birthday of Professor Andrew D. Hamilton FRS has been published featuring contributions from students and colleagues past and present. This article hopes to provide an insight into the rise of a star in molecular recognition, ground breaking discoveries, and on a more light-hearted note, some fond reminiscences of research in Cambridge, Princeton, Pittsburgh, Yale and Oxford.

  14. Dessler, Jimenez, Klein, and Nenes Receive 2012 Atmospheric Sciences Ascent Awards: Citation for Andrew E. Dessler

    NASA Astrophysics Data System (ADS)

    Webster, Peter J.

    2013-11-01

    The Atmospheric Sciences section of AGU awards one of the four Ascent Awards to Professor Andrew E. Dessler of the Department of Atmospheric Sciences at Texas A&M University for fundamental contributions to the understanding of stratospheric-tropospheric exchange processes and the physics of ozone depletion and for attempts to unravel water vapor and cloud feedbacks in the climate system. In addition, he is commended for his ceaseless work in communicating the science of climate change to the public.

  15. 21. View of Andrew McLean Company Textile Mill looking west; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Andrew McLean Company Textile Mill looking west; Building No. 4 at left, Building No. 1 at left center, Building No. 3 at center, Building No. 2 at right center, Building No. 5 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  16. Rapid health needs assessment following hurricane Andrew--Florida and Louisiana, 1992.

    PubMed

    1992-09-18

    Following the impact phase of Hurricane Andrew in Florida (August 24) and Louisiana (August 26) (Figure 1), the primary objectives of the public health response have been to address the health and medical needs of residents in the storm-damaged areas and to provide data for relief interventions and decision-making. This report presents the combined findings from rapid health needs assessment surveys conducted by state health departments with CDC assistance 3-10 days postimpact.

  17. Andrew Wyeth and N.C. Wyeth: a psychodynamic perspective on father and son.

    PubMed

    Shaw, Jon A

    2013-01-01

    This paper examines the relationship between two extraordinary artists, father and son--N.C. Wyeth (1882-1945) and Andrew Wyeth (1917-2009)--and their art. N.C. Wyeth, the father, the most famous illustrator of his day, painted scenes full of drama and action, often of men engaged in violent life and death struggles. N.C. was unable to separate from his powerful mother and yearned for his iconic father. He thought himself an artistic failure and dedicated himself to raising his children to be geniuses. The youngest son, Andrew Wyeth, who lived a "secret life," painted scenes often characterized by pathos: bleak and barren landscapes, leaden skies, tire tracks, gray framed houses, desiccated fields, and circling buzzards. In the father-son relationship, we often seen three themes perpetuated developmentally: (1) the son's identification with the innermost conflicts of his father; (2) the yearning for the iconic father of his youth; and (3) a continuation and disavowal of his father's life. These themes are played out in the relationship between Andrew Wyeth and his father.

  18. Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

    PubMed Central

    Pulleyblank, D E; Shure, M; Tang, D; Vinograd, J; Vosberg, H P

    1975-01-01

    Highly purified nicking-closing enzyme from mouse cells in 20-fold enzyme/substrate excess converts closed circular native PM2, ColE1, and Minicol DNA into limit product sets of DNAs. Each set has a mean degree of supercoiling of approximately zero. The individual species in the sets differ by deltatau = +/-1, +/-2, etc., and the relative masses fit a Boltzmann distribution. It was also demonstrated that "nonsupercoiled" closed circular duplex molecules serve as substrates for the nicking-closing enzyme, and that a distribution of topological isomers is generated. Polynucleotide ligase, acting on nicked circular DNA, forms under the same conditions, the same set of closed DNAs. The latter enzyme freezes the population into sets of molecules otherwise in configurational equilibrium in solution. Images PMID:1060106

  19. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9.

    PubMed

    Sato, Takuya; Sakuma, Tetsushi; Yokonishi, Tetsuhiro; Katagiri, Kumiko; Kamimura, Satoshi; Ogonuki, Narumi; Ogura, Atsuo; Yamamoto, Takashi; Ogawa, Takehiko

    2015-07-14

    Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. PMID:26095606

  20. Ultraselective homogeneous electrochemical biosensor for DNA species related to oral cancer based on nicking endonuclease assisted target recycling amplification.

    PubMed

    Tan, Yue; Wei, Xiaofeng; Zhao, Mengmeng; Qiu, Bin; Guo, Longhua; Lin, Zhenyu; Yang, Huang-Hao

    2015-09-15

    Traditional electrochemical DNA biosensors need DNA immobilization on the electrode surface, which is tedious and time-consuming. In this study, a simple but ultraselective electrochemical DNA biosensor had been designed to determine target DNA species related to oral cancer overexpressed 1 in saliva, which combines the signal amplification of nicking endonuclease assisted target recycling with the immobilization-free electrochemical method. The complementary substrate strand of target DNA species contains a simple asymmetric sequence had been modified with a methylene blue at the 3' terminal first, which cannot diffuse easily to the negative charged ITO electrode surface due to the abundant negative charges. The presence of the target DNA would trigger the formation of double-stranded DNA (dsDNA). Then the nicking endonuclease can recognize the simple asymmetric sequence in the dsDNA and cleave the substrate strand of ds-DNA into two pieces, a long ssDNA and a 2-base ssDNA linked with methylene blue. The short one can diffuse easily to the negative charged ITO electrode surface and results in the enhanced electrochemical response detected. At the same time, the target DNA can dissociate from the dsDNA and trigger the next round of hybridization, cleavage, and releasing, which results in the signal amplification. This homogeneous DNA biosensor can detect as low as 0.35 pM (S/N = 3) target DNA. Compared with the traditional heterogeneous electrochemical DNA biosensors, which are tedious and time-consuming due to the complex DNA immobilization process, the assay not only owns the merits of simple and high efficiency since performed in a homogeneous solution but also exhibits a high distinction ability to single-base mismatch, double-bases mismatch, and noncomplementary DNA sequence.

  1. High sensitive and label-free colorimetric DNA detection based on nicking endonuclease-assisted activation of DNAzymes.

    PubMed

    Li, Juan; Yao, Qiu-Hong; Fu, Hua-E; Zhang, Xiao-Long; Yang, Huang-Hao

    2011-07-15

    Horseradish peroxidase mimicking DNAzyme (HRP-DNAzyme) attracts growing interest as an amplifying label for biorecognition and biosensing events, especially for DNA detection. However, in the traditional designs, one target molecule can only generate one HRP-DNAzyme, which limits the signal enhancement and thus its sensitivity. In this article, we propose an amplified and label-free colorimetric DNA detection strategy based on nicking endonuclease (NEase)-assisted activation of HRP-DNAzymes (NEAA-DNAzymes). This new strategy relies on the hairpin-DNAzyme probe and NEase-assisted target recycling. In the hairpin-DNAzyme probe, the HRP-DNAzyme sequence is protected in a "caged" inactive structure, whereas the loop region includes the target complementary sequence. Upon hybridization with target, the beacon is opened, resulting in the activation of the HRP-DNAzyme. Meanwhile, upon formation of the duplex, the NEase recognizes a specific nucleotide sequence and cleaves the hairpin-DNAzyme probe into two fragments. After nicking, the fragments of the hairpin-DNAzyme probe spontaneously dissociate from the target DNA. Amplification is accomplished by another hairpin-DNAzyme probe hybridizing to the released intact target to continue the strand-scission cycle, which results in activation of numerous DNAzymes. The activated HRP-DNAzymes generate colorimetric or chemiluminescence readout signals, thus providing the amplified detection of DNA. The detection limit of the colorimetric method is 10 pmol/L, which are three orders of magnitude lower than that without NEase. In addition, the detection limit of the chemiluminescence method is 0.2 pmol/L. Meanwhile, this strategy also exhibits high discrimination ability even against single-base mismatch.

  2. A flavin-mononucleotide-binding site in Hansenula anomala nicked flavocytochrome b2, requiring the association of two domains.

    PubMed

    Gervais, M; Labeyrie, F; Risler, Y; Vergnes, O

    1980-10-01

    Previous experiments in our laboratory with Saccharomyces cervisiae flavocytochrom b2 indicated that both fragments alpha and beta of the enzyme after cleavage by yeast proteases are required to form the flavin site. More detailed experiments have not been carried out on the nicked Hansenula anomala enzyme obtained by tryptic cleavage. A method has been devised that gives a quantitative separation in 4 M urea of beta, and alpha with its heme still bound. The characteristics of the various species: isolated alpha and beta and mixed alpha + beta were studied in 4 M urea and after elimination of this reagent by dialysis in the presence of FMN and 2-mercaptoethanol. Several methods, including heme spectroscopy, tryptophan fluorescence, sedimentation studies, and titration of bound flavin, were used. The results indicate that isolated alpha and beta have a folded globular structure after renaturation. The flavin binding to the alpha + beta mixture was important (50-100%) with recovery of the flavodehydrogenase activity. In contrast, binding was not detectable (< 0.5%, Kf > 10 mM) for isolated alpha and beta. As far as mononucleotide binding is concerned, such a cooperative requirement for two folding domains has never been reported in other enzymes. The present results are discussed together with others obtained in our laboratory which demonstrate that, as deduced from their sensitivity to trypsin, the structure of S. cerevisiae and H. anomala flavocytochrome b2 protomers is triglobular 'n-x-beta' (n and x combined within alpha). The tetramer assembly, which remains intact as a nicked enzyme (alpha beta)4 after the first trypsin cleavage, is broken down following a second cleavage of the chain into four cytochrome cores (n) and a functional T-flavodehydrogenase entity, a tetramer of the type (x beta)4. PMID:7439181

  3. Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labelling technique.

    PubMed

    Sreejalekshmi, P; Raghavendra, B S; Subramani, T Siva; Murthy, V Chandrashekara; Jamuna, K V; Prasad, R V; Ravindra, J P; Selvaraju, S

    2011-02-01

    The objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (Bubalus bubalis) ovary using classical histology and nick end labelling technique. Ovaries (n=40) procured from the slaughterhouse were used for the study. The sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) and classical histology (H&E). Those follicles showing ≥ 5% TUNEL positivity (TUNEL assay) and pyknotic nuclei (histology) in granulosa cells were classified as atretic. Based on histology, the atretic primary and secondary follicles (%) were 93.82 and 95.62 respectively. The histology study reveals that the rates (%) of atresia in <1, 1-3, 3-5 mm and >5 mm were 36.90, 40.50, 62.84 and 74.5 respectively. Further the atretic tertiary follicles (%) were significantly lower than the primary and secondary classes of follicles. TUNEL assay reveals that the atretic rate (%) of tertiary follicles in <1, 1-3, 3-5 and ≥ 5 mm class follicles were 50.88, 53.84, 81.81 and 36.36 respectively. The percentage of atresia in >5 mm diameter follicles were significantly lower in TUNEL than histology. Percentages of granulosa and thecal cells positive for atresia by TUNEL were 30.7 ± 0.53 and 13.82 ± 0.18 respectively per follicle. The initial structural changes in atretic follicles were seen primarily in the granulosa cells. In severely atretic follicles TUNEL positive granulosa cells along with theca cells have to be considered in assessing the rate and extent of atresia.

  4. A comment on Andrew C. Papanicolaou's "Beyond Eddington's argument": pragmatism in scientific knowledge and perception.

    PubMed

    Kono, Tetsuya

    2015-03-01

    I agree with Andrew C. Papanicolaou's claim that human beings' cognitive ability can reach to the real world, but don't agree with his claim that the order of this world is akin to our thought. In this commentary paper, I will first defend a recent pragmatic scientific realism called as "entity realism" or "referential realism" which affirms that we are able to reach the real world not by inference but by technological, engineering settings. In the second half of this paper, I will also affirm that our perceptual ability directly reaches the reality too, in referring to the ecological psychology of James J. Gibson.

  5. Emergency mosquito control associated with Hurricane Andrew--Florida and Louisiana, 1992.

    PubMed

    1993-04-01

    Hurricane Andrew crossed south Florida on August 24, 1992 entered the Gulf of Mexico, and struck the Louisiana coast on August 26. In Florida, an estimated 25,000 housing units were destroyed and 37,000 severely damaged in a 200,000-acre area in the southern portion of Dade County; in Louisiana, an estimated 25,000 housing units were destroyed or severely damaged by the storm, primarily in the coastal sections of the 36-parish disaster area. Initial assessment of the disaster areas indicated a need for vector surveillance and control (1). This report summarizes actions to assess and alleviate mosquito-related problems in Florida and Louisiana.

  6. Wireless Andrew.

    ERIC Educational Resources Information Center

    Fickes, Michael

    2000-01-01

    Describes the use of the Internet and laptops help Carnegie Mellon University students carry out sophisticated research anywhere on campus. How the university became a wireless community is discussed. (GR)

  7. One-step highly sensitive florescence detection of T4 polynucleotide kinase activity and biological small molecules by ligation-nicking coupled reaction-mediated signal amplification.

    PubMed

    Chen, Feng; Zhao, Yongxi; Qi, Lin; Fan, Chunhai

    2013-09-15

    DNA phosphorylation, catalyzed by polynucleotide kinase (PNK), plays significant regulatory roles in many biological events. Herein, using T4 PNK as a model target, we describe a one-step, highly sensitive, simple and rapid fluorescence approach for monitoring its activity and inhibition. This innovative strategy is inspired by the great amplification capability of ligation-nicking coupled reaction-mediated signal amplification. In the presence of T4 PNK, one of two short oligonucleotides complementary to the loop sequence of molecular beacon (MB) are phosphorylated, and then ligated with the other by DNA ligase. Upon formation of the stable duplex between the ligated DNA and MB, the fluorescence is restored and further significantly amplified through nicking endonuclease assisted cleavage of multiple MBs. Meanwhile, the cleavage of MBs will also generate new nicks to initiate the ligation reaction. Eventually, a maximum fluorescence enhancement is obtained when the ligation and nicking process reached a dynamic equilibrium. As compared to those of the existing approaches except for the assay based on single nanoparticle counting, all limited to 1:1 signal transduction function, the sensitivity (0.00001U/mL) of the proposed strategy is 100-1700 times higher. The application of the sensing system in complex biological matrix and screening of T4 PNK inhibition are demonstrated with satisfactory results. Moreover, this approach is also successfully used to detect biological small molecules such as adenosine triphosphate (ATP), and can be further extended for nicotinamide adenine dinucleotide (NAD(+)) detection.

  8. Effects of 3'-OH and 5'-PO4 base mispairs and damaged base lesions on the fidelity of nick sealing by Deinococcus radiodurans RNA ligase.

    PubMed

    Schmier, Brad J; Shuman, Stewart

    2014-05-01

    Deinococcus radiodurans RNA ligase (DraRnl) is the founding member of a family of end-joining enzymes encoded by diverse microbes and viruses. DraRnl ligates 3'-OH, 5'-PO4 nicks in double-stranded nucleic acids in which the nick 3'-OH end is RNA. Here we gauge the effects of 3'-OH and 5'-PO4 base mispairs and damaged base lesions on the rate of nick sealing. DraRnl is indifferent to the identity of the 3'-OH nucleobase, provided that it is correctly paired. With 3'-OH mispairs, the DraRnl sealing rate varies widely, with G-T and A-C mispairs being the best substrates and G-G, G-A, and A-A mispairs being the worst. DraRnl accepts 3' A-8-oxoguanine (oxoG) to be correctly paired, while it discriminates against U-oxoG and G-oxoG mispairs. DraRnl displays high activity and low fidelity in sealing 3'-OH ends opposite an 8-oxoadenine lesion. It prefers 3'-OH adenosine when sealing opposite an abasic template site. With 5'-PO4 mispairs, DraRnl seals a 5' T-G mispair as well as it does a 5' C-G pair; in most other respects, the ligation fidelity at 5' mispairs is similar to that at 3' mispairs. DraRnl accepts a 5' A-oxoG end to be correctly paired, yet it is more tolerant of 5' T-oxoG and 5' G-oxoG mispairs than the equivalent configurations on the 3' side of the nick. At 5' nucleobase-abasic site nicks, DraRnl prefers to ligate when the nucleobase is a purine. The biochemical properties of DraRnl are compatible with its participation in the templated repair of RNA damage or in the sealing of filled DNA gaps that have a 3' ribopatch.

  9. James Gregory, the University observatory and the early acquisition of scientific instruments at the University of St Andrews

    PubMed Central

    Rawson, Helen C.

    2015-01-01

    James Gregory, inventor of the reflecting telescope and Fellow of the Royal Society, was the first Regius Professor of Mathematics of the University of St Andrews, 1668–74. He attempted to establish in St Andrews what would, if completed, have been the first purpose-built observatory in the British Isles. He travelled to London in 1673 to purchase instruments for equipping the observatory and improving the teaching and study of natural philosophy and mathematics in the university, seeking the advice of John Flamsteed, later the first Astronomer Royal. This paper considers the observatory initiative and the early acquisition of instruments at the University of St Andrews, with reference to Gregory's correspondence, inventories made ca. 1699–ca. 1718 and extant instruments themselves, some of which predate Gregory's time. It examines the structure and fate of the university observatory, the legacy of Gregory's teaching and endeavours, and the meridian line laid down in 1748 in the University Library.

  10. ANDREWES'S CHRISTMAS FAIRY TALE: ATYPICAL THINKING ABOUT CANCER AETIOLOGY IN 1935.

    PubMed

    Sankaran, Neeraja; van Helvoort, Ton

    2016-06-20

    This paper uses a short 'Christmas fairy-story for oncologists' sent by Christopher Andrewes with a 1935 letter to Peyton Rous as the centrepiece of a reflection on the state of knowledge and speculation about the viral aetiology of cancer in the 1930s. Although explicitly not intended for public circulation at the time, the fairy-story merits publication for its significance in the history of ideas about viruses, which are taken for granted today. Andrewes and Rous were prominent members of the international medical research community and yet faced strong resistance to their theory that viruses could cause such tumours as chicken sarcomas and rabbit papillomas. By looking at exchanges between these men among themselves and other proponents of their theories and with their oncologist detractors, we highlight an episode in the behind-the-scenes workings of medical science and show how informal correspondence helped keep alive a vital but then heterodox idea about the role of viruses in causing cancer. PMID:27386716

  11. Delayed tree mortality in the Atchafalaya Basin of Southern Louisiana following Hurricane Andrew

    USGS Publications Warehouse

    Keeland, B.D.; Gorham, L.E.

    2009-01-01

    Hurricanes can damage trees in forested wetlands, and the potential for mortality related to these storms exists due to the effects of tree damage over time. In August 1992, Hurricane Andrew passed through the forested wetlands of southern Louisiana with winds in excess of 225 kph. Although more than 78 of the basal area was destroyed in some areas, most trees greater than 2.5 cm dbh were alive and resprouting prolifically the following year (98.8). Survival of most tree species was similarly high two years after the hurricane, but mortality rates of some species increased dramatically. For example, Populus heterophylla (swamp cottonwood) mortality increased from 7.8 to 59.2 (n 76) and Salix interior (sandbar willow) mortality increased from 4.5 to 57.1 (n 21). Stem sprouts on many up-rooted hardwood trees of other species were still alive in 1998, 6 years after the hurricane. Due to the understory tree species composition, regeneration, and high levels of resprouting, there was little change in species composition or perhaps a slight shift toward more shade and flood tolerant species six years following the hurricane event. Triadica sebifera (Chinese tallow) was found on some of the sites heavily disturbed by Hurricane Andrew, and may proliferate at the expense of native tree species. ?? 2009 The Society of Wetland Scientists.

  12. Discriminative validity of the MacAndrew Alcoholism Scale with Cluster B personality disorders.

    PubMed

    Smith, S R; Hilsenroth, M J

    2001-06-01

    This study was designed to assess the ability of the Minnesota Multiphasic Personality Inventory (MMPI-2) MacAndrew Alcoholism Scale (MAC-R) to differentiate between outpatients with personality disorders with Substance-Related Disorders (SRDs) and without SRDs. MMPI-2 validity, clinical, and MAC-R scale scores were compared in an SRD Cluster B group (comprised of Narcissistic, Antisocial, Borderline, and Histrionic; n = 15), a non-SRD Cluster B group (n = 33), and a non-SRD group with personality disorders from Clusters A and C (n = 18). Results revealed that the substance-abusing Cluster B group scored significantly higher on the MAC-R ( p <.0001) as well as the Psychopathic Deviate scale ( p <.01). Dimensional analyses illustrated that MAC-R scores were related to the presence of an SRD diagnosis (rpb =.70, p <.0001) and diagnostic criteria for Antisocial Personality Disorder (r =.60, p <.0001). Stepwise regression revealed that (in order of magnitude) the presence of a substance-abuse diagnosis followed by diagnostic criteria for Antisocial and Histrionic Personality Disorders were most related to MAC-R scores (R =.78, R(2) =.60). This indicates that the MAC-R may be more related to the presence of an SRD than has been suggested, and when used in outpatient settings as MacAndrew (1965) intended, the MAC-R may be useful as a screening device for assessing SRD among outpatients with Axis II psychopathology.

  13. Impacts of Hurricane Andrew on carbonate platform environments, northern Great Bahama Bank

    NASA Astrophysics Data System (ADS)

    Boss, Stephen K.; Neumann, A. Conrad

    1993-10-01

    The northern (most energetic) quadrant of Hurricane Andrew (August 1992) passed over leeward-margin sand waves, bank-top sand shoals, reefs, and low islands of Great Bahama Bank for which an extensive prestorm data base exists. A reconnaissance survey seven weeks after Hurricane Andrew evaluated storm impacts on these bank-top settings. Resurveyed seismic profiles showed that positions, dimensions, and orientations of platform sand bodies were unchanged relative to fixed bedrock features. Surveys of reef communities indicated only minor storm-related disturbance. Coral bleaching may be due to storm-induced environmental stress. In addition, storm-wave plucking of boulders from emergent rocky cays resulted in localized crushing of reef biota. On low islands, beach erosion and storm surge were insignificant, and storm damage to Casuarina forests was minor and substrate-specific. Observed minimal hurricane impacts on northern Great Bahama Bank environments lying 10-75 km from the hurricane eye are reconciled by analysis of meteorological data, which show significant weakening of the storm (expressed as a rise in central barometric pressure of ˜20 mbar) during passage across the bank-top. This study demonstrates the importance of specific dynamic aspects of hurricanes (e.g., varying intensity, strength, size, forward speed, duration) which influence their geologic potential, even over relatively short distances along the storm track of an individual hurricane.

  14. ANDREWES'S CHRISTMAS FAIRY TALE: ATYPICAL THINKING ABOUT CANCER AETIOLOGY IN 1935.

    PubMed

    Sankaran, Neeraja; van Helvoort, Ton

    2016-06-20

    This paper uses a short 'Christmas fairy-story for oncologists' sent by Christopher Andrewes with a 1935 letter to Peyton Rous as the centrepiece of a reflection on the state of knowledge and speculation about the viral aetiology of cancer in the 1930s. Although explicitly not intended for public circulation at the time, the fairy-story merits publication for its significance in the history of ideas about viruses, which are taken for granted today. Andrewes and Rous were prominent members of the international medical research community and yet faced strong resistance to their theory that viruses could cause such tumours as chicken sarcomas and rabbit papillomas. By looking at exchanges between these men among themselves and other proponents of their theories and with their oncologist detractors, we highlight an episode in the behind-the-scenes workings of medical science and show how informal correspondence helped keep alive a vital but then heterodox idea about the role of viruses in causing cancer.

  15. Development of pile foundation bias factors using observed behavior of platforms during Hurricane Andrew

    SciTech Connect

    Aggarwal, R.K.; Litton, R.W.; Cornell, C.A.; Tang, W.H.; Chen, J.H.; Murff, J.D.

    1996-12-31

    The performance of more than 3,000 offshore platforms in the Gulf of Mexico was observed during the passage of Hurricane Andrew in August 1992. This event provided an opportunity to test the procedures used for platform analysis and design. A global bias was inferred for overall platform capacity and loads in the Andrew Joint Industry Project (JIP) Phase 1. It was predicted that the pile foundations of several platforms should have failed, but did not. These results indicated that the biases specific to foundation failure modes may be higher than those of jacket failure modes. The biases in predictions of foundation failure modes were therefore investigated further in this study. The work included capacity analysis and calibration of predictions with the observed behavior for 3 jacket platforms and 3 caissons using Bayesian updating. Bias factors for two foundation failure modes, lateral shear and overturning, were determined for each structure. Foundation capacity estimates using conventional methods were found to be conservatively biased overall.

  16. Demographic effects of natural disasters: a case study of Hurricane Andrew.

    PubMed

    Smith, S K; McCarty, C

    1996-05-01

    Many studies have considered the economic, social, and psychological effects of hurricanes, earthquakes, floods, tornadoes, and other natural disasters, but few have considered their demographic effects. In this paper we describe and evaluate a method for measuring the effects of Hurricane Andrew on the housing stock and population distribution in Dade County, Florida. Using information collected through sample surveys and from other data sources, we investigate the extent of housing damages, the number of people forced out of their homes, where they went, how long they stayed, and whether they returned to their prehurricane residences. We conclude that more than half the housing units in Dade County were damaged by Hurricane Andrew; that more than 353,000 people were forced to leave their homes, at least temporarily; and that almost 40,000 people left the county permanently as a direct result of the hurricane. We believe that this study will provide methodological guidance to analysts studying the demographic effects of other large-scale natural disasters.

  17. Ecological evaluation of proposed dredged material from St. Andrew Bay, Florida

    SciTech Connect

    Mayhew, H.L.; Word, J.Q.; Kohn, N.P.; Pinza, M.R.; Karle, L.M.; Ward, J.A.

    1993-10-01

    The US Army Corps of Engineers (USACE), Mobile District, requested that the Battelle/Marine Sciences Laboratory (MSL) conduct field sampling and chemical and biological testing to determine the suitability of potential dredged material for open ocean disposal. Sediment from St. Andrew Bay was chemically characterized and evaluated for biological toxicity and bioaccumulation of contaminants. The Tier III guidance for ocean disposal testing requires tests of water column effects (following dredged material disposal), deposited sediment toxicity, and bioaccumulation of contaminants from deposited sediment (dredged material). To meet these requirements, the MSL conducted suspended-particulate-phase (SPP) toxicity tests, solid-phase toxicity tests, and bioaccumulation testing on sediment representing potential dredged material from Panama City Harbor. Physical and chemical characterization of sediment to support toxicity and bioaccumulation results was also conducted on both the test and reference sediments. The MSL collected sediment samples from five sites in St. Andrew Bay and one reference site near Lands End Peninsula. The five test sediments and the reference sediment were analyzed for physical and chemical sediment characteristics, SPP chemical contaminants, solid-phase toxicity, SPP toxicity, and bioaccumulation of contaminants.

  18. Source emission testing of the medical waste incinerator, Andrews Air Force Base, Maryland. Final report, 8-9 July 1992

    SciTech Connect

    O'Brien, R.J.

    1992-12-01

    Source emission testing for particulate matter and hydrogen chloride was conducted on the medical waste incinerator located at Bldg 1055, Andrews AFB MD. Compliance standards are found in Temporary Operating Permit No. 16-0655-2-0116N, issued by the State of Maryland on 20 October 1991. Test results indicate incinerator emissions are above the state standard for particulate matter and below the state standard for hydrogen chloride. Recommendations are made to reduce particulate emissions and to retest.... Particulate matter, Hydrogen chloride, Andrews AFB, Medical waste incinerator, Source emission testing.

  19. Ultrasensitive DNA detection by cycle isothermal amplification based on nicking endonuclease and its application to logic gates.

    PubMed

    Li, Xuemei; Ding, Tianrong; Sun, Li; Mao, Changming

    2011-12-15

    In recent years, an intense interest has grown in the DNA logic gates having high potential for computation at literally the "nano-size" level. A limitation of traditional DNA logic gates is that each target strand hybridizes with only a single copy of the probe. This 1:1 hybridization radio limits the gain of the approach and thus its sensitivity. The exponential amplification of nucleic acids has become a core technology in medical diagnostics and has been widely used for the construction of DNA sensor, DNA nanomachine and DNA sequencing. It would be of great interest to develop DNA-based logic systems with exponential amplification for the output signal. In the present study, a series of three-input DNA logic gates with the cycle isothermal amplification based on nicking endonuclease (NEase) are designed. Very low concentrations of the analytes were sufficient to initiate an autocatalytic cascade, achieving a significant improvement of the detection limit, 100-fold improvement compared to the non-autocatalytic system. This was achieved by engineering a simple and flexible biological circuit designed to initiate a cascade of events to detect and amplify a specific DNA sequence. This procedure has the potential to greatly simplify the logic operation because amplification can be performed in "one-pot".

  20. Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata).

    PubMed

    Johnston, S D; López-Fernández, C; Gosálbez, A; Holt, W V; Gosálvez, J

    2009-01-01

    Prototherian spermatozoa are unique amongst the Mammalia in terms of their filiform morphology, tandem arrangement of chromosomes and formation of sperm bundles. In the present study, we provide observations of echidna spermatozoa and note that the superstructure of the bundle is engineered around the shape of the individual sperm head and that this in turn may be a consequence of the unusual circumferential and helicoidal condensation of the DNA during spermiogenesis. Frozen-thawed ejaculated echidna spermatozoa were incubated and examined for the presence of non-typical DNA conformation by means of in situ labelling of DNA breaks using Klenow polymerase and via alkaline single-cell comet assays for detection of fragmented DNA. Both techniques successfully revealed the presence of what appeared to be directional DNA nicking, co-localised with the presence of highly sensitive alkali sites along the length of the sperm nucleus. It was not possible to define whether these alternative DNA configurations were associated with a failure of the sperm nucleus to condense appropriately during spermiogenesis or were evidence of DNA fragmentation following post-thaw incubation or a sequential structural chromatin rearrangement necessary for fertilisation.

  1. Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata).

    PubMed

    Johnston, S D; López-Fernández, C; Gosálbez, A; Holt, W V; Gosálvez, J

    2009-01-01

    Prototherian spermatozoa are unique amongst the Mammalia in terms of their filiform morphology, tandem arrangement of chromosomes and formation of sperm bundles. In the present study, we provide observations of echidna spermatozoa and note that the superstructure of the bundle is engineered around the shape of the individual sperm head and that this in turn may be a consequence of the unusual circumferential and helicoidal condensation of the DNA during spermiogenesis. Frozen-thawed ejaculated echidna spermatozoa were incubated and examined for the presence of non-typical DNA conformation by means of in situ labelling of DNA breaks using Klenow polymerase and via alkaline single-cell comet assays for detection of fragmented DNA. Both techniques successfully revealed the presence of what appeared to be directional DNA nicking, co-localised with the presence of highly sensitive alkali sites along the length of the sperm nucleus. It was not possible to define whether these alternative DNA configurations were associated with a failure of the sperm nucleus to condense appropriately during spermiogenesis or were evidence of DNA fragmentation following post-thaw incubation or a sequential structural chromatin rearrangement necessary for fertilisation. PMID:19874725

  2. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  3. [The Andrew Heiskell Library for the Blind and Physically Handicapped.] A Library with a Difference. Projects and Experiments.

    ERIC Educational Resources Information Center

    Jarkon, Joe; Fitzpatrick, Vicki, Ed.

    This publication describes the Andrew Heiskell Library for the Blind and Physically Handicapped, a regional library for the National Library Service (NLS) example of the creative use of physical space and innovative technology. The publication focuses on the materials-handling system designed for the new facility, including system design…

  4. 75 FR 81637 - Endangered and Threatened Wildlife and Plants; Notice of Availability of the St. Andrew Beach...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... (63 FR 70053). The endangered St. Andrew beach mouse is now found in two populations: East Crooked... recovery plan available for public comment from April 22, 2009 through June 22, 2009 (74 FR 18403). We... feral cats and hogs in beach mouse habitat. 4. In areas with known populations of beach mice...

  5. Should Community College Be Free? Forum. "Education Next" Talks with Sara Goldrick-Rab and Andrew P. Kelly

    ERIC Educational Resources Information Center

    Goldrick-Rab, Sara; Kelly, Andrew P.

    2016-01-01

    In this article, "Education Next" talks with Sara Goldrick-Rab and Andrew Kelly. President Obama's proposal for tuition-free community college, seems to have laid down a marker for the Democratic Party. Vermont senator Bernie Sanders is touting his plan for free four-year public college on the primary trail; Massachusetts senator…

  6. The Psychological Effects of Hurricane Andrew on Ethnic Minority and Caucasian Children and Adolescents: A Case Study.

    ERIC Educational Resources Information Center

    Jones, Russell T.; Frary, Robert; Cunningham, Phillippe; Weddle, J. David; Kaiser, Lisa

    2001-01-01

    Several measures of children's reactions to disasters were employed with 212 elementary and middle school students 6 months after experiencing Hurricane Andrew. Higher levels of intrusive symptomatology were found for girls and for elementary school children. Discusses lack of findings concerning race and implications for future research.…

  7. Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.).

    PubMed

    Divakaran, Minoo; Babu, K Nirmal; Peter, K V

    2016-01-01

    Vanilla (Vanilla planifolia Andrews (syn. V. fragrans Salisb.), a native of Central America, is the primary source of natural vanillin and plays a major role in the global economy. The gene pool of vanilla is threatened by deforestation and overcollection that has resulted in disappearance of natural habitats and wild species. Continuous vegetative propagation and lack of natural seed set and sufficient variations in the gene pool hamper crop improvement programs. In vitro techniques, one of the key tools of plant biotechnology, can be employed for overcoming specific problems, viz. production of disease-free clones, inducing somaclonal variations, developing hybrids, gene pool conservation, incorporating desired traits by distant hybridization, genetic engineering, etc. However, realization of these objectives necessitates standardization of protocols. This chapter describes the various protocols optimized for crop improvement in Vanilla species. PMID:27108309

  8. The psychometric assessment of alcoholism in forensic groups: the MacAndrew scale and response bias.

    PubMed

    Wasyliw, O E; Haywood, T W; Grossman, L S; Cavanaugh, J L

    1993-04-01

    Although the MacAndrew Alcoholism scale is the most widely used Minnesota Multiphasic Personality Inventory (MMPI) measure of vulnerability to alcohol abuse, its accuracy has not been studied in patients intrinsically motivated to exaggerate or minimize psychopathology. We examined the usefulness of the MAC in predicting alcohol abuse in a forensic clinical sample. Results indicate the MAC (a) was not more effective than direct inquiry in this group, (b) scores were correlated negatively with minimization and positively with exaggeration for subjects with histories of alcohol abuse, (c) offered advantages over direct inquiry both in screening for alcohol history (sensitivity) and in confirming it (specificity), and (d) scores were only moderately more accurate in valid than in minimized or exaggerated MMPI protocols. The results suggest that clinicians should use the MAC cautiously, particularly when they suspect motivation to minimize psychopathology.

  9. STS-89 M.S. Andrew Thomas, poses the day before launch

    NASA Technical Reports Server (NTRS)

    1998-01-01

    STS-89 Mission Specialist Andrew Thomas, Ph.D., poses at KSC's Launch Pad 39A wearing a miniature koala bear on the day before the scheduled launch of Space Shuttle Endeavour that will carry him up to the Russian Space Station Mir. Final preparations are under way toward liftoff on Jan. 22 on the eighth mission to dock with Mir. After docking, Dr. Thomas will transfer to the space station, succeeding David Wolf, M.D., who will return to Earth aboard Endeavour. Dr. Thomas, who was born and educated in South Australia, will live and work on Mir until June. STS-89 is scheduled for liftoff at 9:48 p.m. EST.

  10. Prosthetic rehabilitation of severe Siebert's Class III defect with modified Andrews bridge system.

    PubMed

    Rathee, Manu; Sikka, Neha; Jindal, Sahil; Kaushik, Ashutosh

    2015-03-01

    Prosthetic dentistry involves the replacement of missing and contiguous tissues with artificial substitutes to restore and maintain the oral functions, appearance, and health of the patient. The treatment of edentulous areas with ridge defects poses a challenging task for the dentist. Management of such cases involves a wide range of treatment options comprising mainly of surgical interventions and non surgical techniques such as use of removable, fixed or fixed- removable partial dentures. But each treatment plan undertaken should be customized according to patient needs. A variety of factors such as quality and quantity of existing contiguous hard and soft tissues, systemic condition and economic status of the patient play an important role in treatment planning, clinical outcome and prognosis. This case report presents the restoration of a Seibert's Class III ridge defect by an economical modification of Andrews Bridge in a 32 Year old patient. PMID:25821362

  11. Prosthetic rehabilitation of severe Siebert's Class III defect with modified Andrews bridge system.

    PubMed

    Rathee, Manu; Sikka, Neha; Jindal, Sahil; Kaushik, Ashutosh

    2015-03-01

    Prosthetic dentistry involves the replacement of missing and contiguous tissues with artificial substitutes to restore and maintain the oral functions, appearance, and health of the patient. The treatment of edentulous areas with ridge defects poses a challenging task for the dentist. Management of such cases involves a wide range of treatment options comprising mainly of surgical interventions and non surgical techniques such as use of removable, fixed or fixed- removable partial dentures. But each treatment plan undertaken should be customized according to patient needs. A variety of factors such as quality and quantity of existing contiguous hard and soft tissues, systemic condition and economic status of the patient play an important role in treatment planning, clinical outcome and prognosis. This case report presents the restoration of a Seibert's Class III ridge defect by an economical modification of Andrews Bridge in a 32 Year old patient.

  12. Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.).

    PubMed

    Divakaran, Minoo; Babu, K Nirmal; Peter, K V

    2016-01-01

    Vanilla (Vanilla planifolia Andrews (syn. V. fragrans Salisb.), a native of Central America, is the primary source of natural vanillin and plays a major role in the global economy. The gene pool of vanilla is threatened by deforestation and overcollection that has resulted in disappearance of natural habitats and wild species. Continuous vegetative propagation and lack of natural seed set and sufficient variations in the gene pool hamper crop improvement programs. In vitro techniques, one of the key tools of plant biotechnology, can be employed for overcoming specific problems, viz. production of disease-free clones, inducing somaclonal variations, developing hybrids, gene pool conservation, incorporating desired traits by distant hybridization, genetic engineering, etc. However, realization of these objectives necessitates standardization of protocols. This chapter describes the various protocols optimized for crop improvement in Vanilla species.

  13. A conversation with Andrew Benson: reflections on the discovery of the Calvin-Benson cycle.

    PubMed

    Benson, Andrew

    2013-03-01

    On June 26-27, 2012, one of us (BBB) made a video based on an interview conducted with Andrew A. Benson, Professor Emeritus, Scripps Institution of Oceanography, University of California, San Diego, CA, USA. The video was first shown in a seminar presented by BBB on July 27, 2012 at the Calvin Laboratory, University of California, Berkeley, to mark the departure of the Energy Biosciences Institute to a new building. Here we record the conversation taking place during the interview. The Brancraft Library on the Berkeley campus will house the video's transcript in its oral histories collection, and the video will be housed in its motion picture collection. The video and the transcript have also been posted on You Tube (http://youtu.be/GfQQJ2vR_xE).

  14. The post-disaster negative health legacy: pregnancy outcomes in Louisiana after Hurricane Andrew.

    PubMed

    Antipova, Anzhelika; Curtis, Andrew

    2015-10-01

    Disasters and displacement increasingly affect and challenge urban settings. How do pregnant women fare in the aftermath of a major disaster? This paper investigates the effect of pregnancies in disaster situations. The study tests a hypothesis that pregnant women residing in hurricane-prone areas suffer higher health risks. The setting is Louisiana in the Gulf Coast, United States, a state that continually experiences hurricane impacts. The time period for the analysis is three years following the landfall of Hurricane Andrew in 1992. We analysed low birth weight and preterm deliveries before and after landfall, as a whole and by race. Findings support an association between hazards and health of a community and indicate that pregnant women in the affected area, irrespective of race, are more likely to experience preterm deliveries compared to pre-event births. Results suggest there is a negative health legacy impact in Louisiana as a result of hurricane landfall.

  15. Business closure and relocation: a comparative analysis of the Loma Prieta earthquake and Hurricane Andrew.

    PubMed

    Wasileski, Gabriela; Rodríguez, Havidán; Diaz, Walter

    2011-01-01

    The occurrence of a number of large-scale disasters or catastrophes in recent years, including the Indian Ocean tsunami (2004), the Kashmir earthquake (2005), Hurricane Katrina (2005) and Hurricane Ike (2008), have raised our awareness regarding the devastating effects of disasters on human populations and the importance of developing mitigation and preparedness strategies to limit the consequences of such events. However, there is still a dearth of social science research focusing on the socio-economic impact of disasters on businesses in the United States. This paper contributes to this research literature by focusing on the impact of disasters on business closure and relocation through the use of multivariate logistic regression models, specifically focusing on the Loma Prieta earthquake (1989) and Hurricane Andrew (1992). Using a multivariate model, we examine how physical damage to the infrastructure, lifeline disruption and business characteristics, among others, impact business closure and relocation following major disasters.

  16. Prosthetic rehabilitation of severe Siebert's Class III defect with modified Andrews bridge system

    PubMed Central

    Rathee, Manu; Sikka, Neha; Jindal, Sahil; Kaushik, Ashutosh

    2015-01-01

    Prosthetic dentistry involves the replacement of missing and contiguous tissues with artificial substitutes to restore and maintain the oral functions, appearance, and health of the patient. The treatment of edentulous areas with ridge defects poses a challenging task for the dentist. Management of such cases involves a wide range of treatment options comprising mainly of surgical interventions and non surgical techniques such as use of removable, fixed or fixed- removable partial dentures. But each treatment plan undertaken should be customized according to patient needs. A variety of factors such as quality and quantity of existing contiguous hard and soft tissues, systemic condition and economic status of the patient play an important role in treatment planning, clinical outcome and prognosis. This case report presents the restoration of a Seibert's Class III ridge defect by an economical modification of Andrews Bridge in a 32 Year old patient. PMID:25821362

  17. Children's predisaster functioning as a predictor of posttraumatic stress following Hurricane Andrew.

    PubMed

    La Greca, A M; Silverman, W K; Wasserstein, S B

    1998-12-01

    This study examined (a) children's predisaster behavioral and academic functioning as a predictor of posttraumatic stress (PTS) following Hurricane Andrew and (b) whether children who were exposed to the disaster would display a worsening of prior functioning. Fifteen months before the disaster, 92 4th through 6th graders provided self-reports of anxiety; peers and teachers rated behavior problems (anxiety, inattention, and conduct) and academic skills. Measures were repeated 3 months postdisaster; children also reported PTS symptoms and hurricane-related experiences (i.e., exposure). PTS symptoms were again assessed 7 months postdisaster. At 3 months postdisaster, children's exposure to the disaster, as well as predisaster ratings of anxiety, inattention, and academic skills, predicted PTS symptoms. By 7 months, only exposure, African American ethnicity, and predisaster anxiety predicted PTS. Prior anxiety levels also worsened as a result of exposure to the disaster. The findings have implications for identifying and treating children at risk for stress reactions following a catastrophic disaster.

  18. Stability and change in stress, resources, and psychological distress following natural disaster: Findings from hurricane Andrew.

    PubMed

    Norris, F H; Perilla, J L; Riad, J K; Kaniasty, K; Lavizzo, E A

    1999-01-01

    Abstract The stress, resource, and symptom levels of 241 residents of southern Dade County, Florida were assessed 6 and 30 months after Hurricane Andrew. Percentages meeting study criteria for depression and PTSD did not change over time. Whereas mean levels of intrusion and arousal decreased, depressive symptoms remained stable, and avoidance/numbing symptoms actually increased. Intrusion and arousal were associated more strongly with pre-disaster factors (gender, ethnicity) and within-disaster factors (injury, property loss) than with post-disaster factors (stress, resources), but the reverse was true for depression and avoidance. Changes over time in symptoms were largely explained by changes over time in stress and resources. The findings indicate that ongoing services are needed to supplement the crisis-oriented assistance typically offered to disaster victims.

  19. Symptoms of posttraumatic stress in children after Hurricane Andrew: a prospective study.

    PubMed

    La Greca, A; Silverman, W K; Vernberg, E M; Prinstein, M J

    1996-08-01

    The authors examined symptoms of posttraumatic stress in 3rd-5th grade children during the school year after Hurricane Andrew. From a conceptual model of the effects of traumatic events, 442 children were evaluated 3, 7, and 10 months postdisaster with respect to (a) their exposure to traumatic events during and after the disaster, (b) their preexisting demographic characteristics, (c) the occurrence of major life stressors, (d) the availability of social support, and (e) the type of coping strategies used to cope with disaster-related distress. Although symptoms of posttraumatic stress disorder (PTSD) declined over time, a substantial level of symptomatology was observed up to 10 months after the disease. All 5 factors in the conceptual model were predictive of children's PTSD symptoms 7 and 10 months postdisaster. Findings are discussed in terms of the potential utility of the model for organizing thinking about factors that predict the emergence and persistence of PTSD symptoms in children.

  20. Comprehensive assessment of health needs 2 months after Hurricane Andrew--Dade County, Florida, 1992.

    PubMed

    1993-06-11

    On August 24, 1992, Hurricane Andrew struck southern Florida. More than 28,000 houses, mobile homes, and apartment buildings were destroyed, and approximately 107,000 additional dwellings sustained major damage. An estimated 180,000 persons were left homeless; insured damages were estimated at $15.5 billion and total damages at more than $30 billion. During the recovery period, many private and public health-care facilities damaged or destroyed in the storm were not functional. During November 3-13, to help prioritize health needs and direct public health resources, the Dade County Public Health Unit of the Florida Department of Health and Rehabilitative Services conducted a survey to assess health needs and the availability of health-care services during the recovery phase with funds provided by the Federal Emergency Management Agency (FEMA). This report summarizes the results of the survey.

  1. Physical symptoms of chronic fatigue syndrome are exacerbated by the stress of Hurricane Andrew.

    PubMed

    Lutgendorf, S K; Antoni, M H; Ironson, G; Fletcher, M A; Penedo, F; Baum, A; Schneiderman, N; Klimas, N

    1995-01-01

    This study examined the effects of Hurricane Andrew on physical symptoms and functional impairments in a sample of chronic fatigue syndrome (CFS) patients residing in South Florida. In the months after Hurricane Andrew (September 15-December 31, 1992), 49 CFS patients were assessed for psychosocial and physical functioning with questionnaires, interviews, and physical examinations. This sample was made up of 25 CFS patients living in Dade county, a high impact area, and 24 patients in Broward and Palm Beach counties, areas less affected by the hurricane. Based on our model for stress-related effects on CFS, we tested the hypothesis that the patients who had the greatest exposure to this natural disaster would show the greatest exacerbation in CFS symptoms and related impairments in activities of daily living (illness burden). In support of this hypothesis, we found that the Dade county patients showed significant increases in physician-rated clinical relapses and exacerbations in frequency of several categories of self-reported CFS physical symptoms as compared to the Broward/Palm Beach county patients. Illness burden, as measured on the Sickness Impact Profile, also showed a significant increase in the Dade county patients. Although extent of disruption due to the storm was a significant factor in predicting relapse, the patient's posthurricane distress response was the single strongest predictor of the likelihood and severity of relapse and functional impairment. Additionally, optimism and social support were significantly associated with lower illness burden after the hurricane, above and beyond storm-related disruption and distress responses. These findings provide information on the impact of environmental stressors and psychosocial factors in the exacerbation of CFS symptoms.

  2. A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease

    PubMed Central

    Guan, Chudi; Kumar, Sanjay

    2005-01-01

    A stable heterodimeric protein containing a single correctly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing intein system. As predicted by a model presented earlier, purified SCD protein acts a non-specific nicking endonuclease on normal linear DNA. The SCD retains some ability to recognize and cleave a deviated DNA double-helix near a nick or a strand-crossing site. Thus, we infer that the non-specific and nicked-site cleavage activities observed for the native T7 endonuclease I (as distinct from the resolution activity) are due to uncoordinated actions of the catalytic domains. The positively charged C-terminus of T7 Endo I is essential for the enzymatic activity of SCD, as it is for the native enzyme. We propose that the preference of the native enzyme for the resolution reaction is achieved by cooperativity in the binding of its two catalytic domains when presented with two of the arms across a four-way junction or cruciform structure. PMID:16264086

  3. Simulation of Water Balance and Forest Treatment Effects at the H.J. Andrews Experimental Forest

    SciTech Connect

    Waichler, Scott R.; Wemple, Beverley C.; Wigmosta, Mark S.

    2005-10-30

    The watershed model DHSVM was applied to the small watersheds WS1,2,3 in H.J. Andrews Experimental Forest (HJA), Oregon and tested for skill in simulating observed forest treatment effects on streamflow. These watersheds in the rain-snow transition zone underwent road and clearcut treatments during 1959-66 and subsequent natural regeneration. DHSVM was applied with 10 m and 1 hr resolution to 1958-98, most of the period of record. Water balance for old-growth WS2 indicated that evapotranspiration and streamflow were unlikely to be the only loss terms, and groundwater recharge was included to account for about 12% of precipitation; this term was assumed zero in previous studies. After limited calibration, overall efficiency in simulating hourly streamflow exceeded 0.7, and mean annual error was less than 10%. Model skill decreased at the margins, with overprediction of low flows and underprediction of high flows. However, statistical analyses of simulated and observed peakflows yielded similar characterizations of treatment effects. Primary simulation weaknesses were snowpack accumulation, snowmelt under rain-on-snow conditions, and production of quickflow. This challenging test of DHSVM moved the model closer to a practical tool for forest management.

  4. Unsaturated flow modeling for performance assessment of a radioactive waste disposal facility in Andrews County, Texas

    NASA Astrophysics Data System (ADS)

    Martinez Baquero, G. F.; Singh, A.; Holt, R. M.; grisak, G. E.

    2011-12-01

    Quantitifying infiltration rates is a key component of the performance assessment for radioactive waste disposal facilities. In arid regions with scarce infiltration data, this is a challenging problem because of the computational limitations of available numerical implementations to solve water flow and transport equations. This work summarizes methodology and analysis performed to overcome some of these challenges and to generate infiltration scenarios for a low level waste disposal site in Andrews County, Texas. The work presented here includes preparation of a two dimensional finite element model in HYDRUS that includes the cover system and adjacent geologic units, calibration of hydraulic properties and root water uptake parameters based on soft information, preparation of atmospheric forcings based on current and hypothesized future climatic conditions, evaluation of impacts related to temporal and spatial discretization of forcings and model domain, and definition of scenarios for cover degradation and wetter climate conditions. Results of this work include a sensitivity analysis of infiltration rates to changes in boundary conditions under quasi-steady state, evaluation of the impact of temporal discretization of the atmospheric forcings in terms of water balance error and computational efficiency, and the estimation of infiltration rates under different scenarios. Infiltration rates from this work are being incorporated into a transport model to estimate potential radiological doses based on performance assessment modeling analyses. Findings from this work seek to contribute towards robust approaches to estimate infiltration in arid regions.

  5. Occurrence of Cucumber mosaic virus on vanilla (Vanilla planifolia Andrews) in India.

    PubMed

    Madhubala, R; Bhadramurthy, V; Bhat, A I; Hareesh, P S; Retheesh, S T; Bhai, R S

    2005-06-01

    Cucumber mosaic virus (CMV) causing mosaic, leaf distortion and stunting of vanilla (Vanilla planifolia Andrews) in India was characterized on the basis of biological and coat protein (CP) nucleotide sequence properties. In mechanical inoculation tests, the virus was found to infect members of Chenopodiaceae, Cucurbitaceae, Fabaceae and Solanaceae. Nicotiana benthamiana was found to be a suitable host for the propagation of CMV. The virus was purified from inoculated N. benthamiana plants and negatively stained purified preparations contained isometric particles of about 28 nm in diameter. The molecular weight of the viral coat protein subunits was found to be 25.0 kDa. Polyclonal antiserum was produced in New Zealand white rabbit, immunoglobulin G (IgG) was purified and conjugated with alkaline phosphatase enzyme. Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) method was standardized for the detection of CMV infection in vanilla plants. CP gene of the virus was amplified using reverse transcriptase-polymerase chain reaction (RT-PCR), cloned and sequenced. Sequenced region contained a single open reading frame of 657 nucleotides potentially coding for 218 amino acids. Sequence analyses with other CMV isolates revealed the greatest identity with black pepper isolate of CMV (99%) and the phylogram clearly showed that CMV infecting vanilla belongs to subgroup IB. This is the first report of occurrence of CMV on V. planifolia from India.

  6. Wind damage effects of Hurricane Andrew on mangrove communities along the southwest coast of Florida, USA

    USGS Publications Warehouse

    Doyle, T.W.; Smith, T. J.; Robblee, M.B.

    1995-01-01

    On August 24, 1992, Hurricane Andrew downed and defoliated an extensive swath of mangrove trees across the lower Florida peninsula. Permanent field sites were established to assess the extent of forest damage and to monitor the rate and process of forest recovery. Canopy trees suffered the highest mortality particularly for sites within and immediately north of the storm's eyewall. The type and extent of site damage, windthrow, branch loss, and defoliation generally decreased exponentially with increasing distance from the storm track. Forest damage was greater for sites in the storm's right quadrant than in the left quadrant tor the same given distance from the storm center. Stand exposure, both horizontally and vertically, increased the susceptibility and probability of forest damage and accounted for much of the local variability. Slight species differences were found. Laguncularia racemosa exceeded Avicennia germinans and Rhizophora mangle in damage tendency under similar wind conditions. Azimuths of downed trees were strongly correlated with maximum wind speed and vector based on a hurricane simulation of the storm. Lateral branch loss and leaf defoliation on sites without windthrow damage indicated a degree of crown thinning and light penetration equivalent to treefall gaps under normally intact forest conditions. Mangrove species and forests are susceptible to catastrophic disturbance by hurricanes; the impacts of which are significant to changes in forest structure and function.

  7. Genomewide scan for adaptive differentiation along altitudinal gradient in the Andrew's toad Bufo andrewsi.

    PubMed

    Guo, Baocheng; Lu, Di; Liao, Wen Bo; Merilä, Juha

    2016-08-01

    Recent studies of humans, dogs and rodents have started to discover the genetic underpinnings of high altitude adaptations, yet amphibians have received little attention in this respect. To identify possible signatures of adaptation to altitude, we performed a genome scan of 15 557 single nucleotide polymorphisms (SNPs) obtained with restriction site-associated DNA sequencing of pooled samples from 11 populations of Andrew's toad (Bufo andrewsi) from the edge of the Tibetan Plateau, spanning an altitudinal gradient from 1690 to 2768 m.a.s.l. We discovered significant geographic differentiation among all sites, with an average FST   = 0.023 across all SNPs. Apart from clear patterns of isolation by distance, we discovered numerous outlier SNPs showing strong associations with variation in altitude (1394 SNPs), average annual temperature (1859 SNPs) or both (1051 SNPs). Levels and patterns of genetic differentiation in these SNPs were consistent with the hypothesis that they have been subject to directional selection and reflect adaptation to altitudinal variation among the study sites. Genes with footprints of selection were significantly enriched in binding and metabolic processes. Several genes potentially related to high altitude adaptation were identified, although the identity and functional significance of most genomic targets of selection remain unknown. In general, the results provide genomic support for results of earlier common garden and low coverage genetic studies that have uncovered substantial adaptive differentiation along altitudinal and latitudinal gradients in amphibians. PMID:27289071

  8. Long-term effects of Hurricane Andrew: revisiting mental health indicators.

    PubMed

    McDonnell, S; Troiano, R P; Barker, N; Noji, E; Hlady, W G; Hopkins, R

    1995-09-01

    Two population-based surveys of South Dade County, Florida, were conducted after Hurricane Andrew to compare hurricane-related symptoms of mental distress and describe the impact of mental health outreach teams. Households were selected by three-stage cluster sampling and findings from the two surveys, 13 months apart, were compared. Response rates were 75 per cent and 84 per cent. The prevalence of symptoms of mental distress decreased over time. However, in the households contacted by the teams (25 per cent of sample), the prevalence of symptoms (50 per cent) did not differ from households not contacted (43 per cent). Households contacted by teams that reported symptoms were just as likely to have been referred for help by the teams (72 per cent) as those without symptoms (68 per cent). Households reporting symptoms were equally likely to get counselling regardless of whether the teams visited. Mental health teams had no significant impact on mental health symptoms or the use of mental health services. Alternative approaches to mental health outreach teams need to be explored.

  9. Storm-tide elevations produced by Hurricane Andrew along the southern Florida coasts, August 24, 1992

    USGS Publications Warehouse

    Murray, Mitchell H.

    1994-01-01

    On August 24, 1992, Hurricane Andrew crossed southern peninsular Florida. The combined effects of storm surge from the hurricane and astronomical tide, referred to as storm tide, caused flooding over a large part of southern Florida. Subsequent to the flooding, many high-water marks were identified, described, and surveyed along the south- eastern coast of Florida (Miami to Key Largo) and at selected areas along the southwestern coast of Florida (Flamingo to Goodland). Descriptions of these 336 high-water makrs are presented in tabular form in this report and their locations are plotted on nineteen 7.5-minute topographic quadrangle maps. For the southeastern coast, north-south profiles of the high-water makrs along the outher and inner barrier islands and the western shoreline of Biscayne Bay are presented. Average storm-tide elevations (relative to sea level) ranged from 4 to 6 feet in northern Biscayne Bay, were as much as 17 feet on the western shoreline near the center of the bay and ranged from 3 to 6 feet in southern Biscayne Bay and Barnes Sound. Storm-tide elevations along the southwestern coast ranged from 4 to 5 feet at Flamingo and 5 to 7 feet at Goodland in the Ten Thousand Islands area.

  10. Weathering the storm: children's long-term recall of Hurricane Andrew.

    PubMed

    Fivush, Robyn; Sales, Jessica McDermott; Goldberg, Amy; Bahrick, Lorraine; Parker, Janat

    2004-01-01

    Children who experienced a highly stressful natural disaster, Hurricane Andrew, were interviewed within a few months of the event, when they were 3-4 years old, and again 6 years later, when they were 9-10 years old. Children were grouped into low, moderate, or high stress groups depending on the severity of the experienced storm. All children were able to recall this event in vivid detail 6 years later. In fact, children reported over twice as many propositions at the second interview as at the first. At the initial interview, children in the high stress group reported less information than children in the moderate stress group, but 6 years later, children in all three stress groups reported similar amounts of information. However children in the high stress group needed more questions and prompts than children in the other stress groups. Yet children in the high stress group also reported more consistent information between the two interviews, especially about the storm, than children in the other stress groups. Implications for children's developing memory of stressful events are discussed.

  11. Fluorescence biosensing strategy based on mercury ion-mediated DNA conformational switch and nicking enzyme-assisted cycling amplification for highly sensitive detection of carbamate pesticide.

    PubMed

    Wang, Xiuzhong; Hou, Ting; Dong, Shanshan; Liu, Xiaojuan; Li, Feng

    2016-03-15

    Pesticides are of great importance in agricultural and biological fields, but pesticide residues may harm the environment and human health. A highly sensitive fluorescent biosensor for the detection of carbamate pesticide has been developed based on acetylcholinesterase (AChE)-catalyzed hydrolysis product triggered Hg(2+) release coupled with subsequent nicking enzyme-induced cleavage of a duplex DNA for cycling amplification. In this protocol, two DNA probes, an unmodified single-stranded helper DNA probe 1 (HP1) and a quencher-fluorophore probe (QFP) are ingeniously designed. HP1 can be folded into hairpin configuration through T-Hg(2+)-T base pair formation. QFP, labeled with FAM and BHQ1 at its two terminals, contains the recognition sequence and the cleavage site of the nicking enzyme. In the presence of carbamate pesticide, the activity of AChE is inhibited, and the amount of the product containing the thiol group generated by the hydrolysis reaction of acetylthiocholine chloride (ACh) decreases, resulting in the release of a low concentration of Hg(2+). The number of HP1 that can be selectively unfolded would be reduced and the subsequent nicking enzyme-assisted cleavage processes would be affected, resulting in decreased fluorescence signals. The fluorescence intensity further decreases with the increase of the pesticide concentration. Therefore, the pesticide content can be easily obtained by monitoring the fluorescence signal change, which is inversely proportional to the logarithm of the pesticide concentration. The detection limit of aldicarb, the model analyte, is 3.3 μgL(-1), which is much lower than the Chinese National Standards or those previously reported. The as-proposed method has also been applied to detect carbamate pesticide residues in fresh ginger and artificial lake water samples with satisfactory results, which demonstrates that the method has great potential for practical application in biological or food safety field.

  12. Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.

    PubMed

    Gabsalilow, Lilia; Schierling, Benno; Friedhoff, Peter; Pingoud, Alfred; Wende, Wolfgang

    2013-04-01

    Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone NHEJ can be efficiently suppressed by 'nickases' that produce a single-strand break rather than a double-strand break. Highly specific nickases have been produced by engineering of homing endonucleases and more recently by modifying zinc finger nucleases (ZFNs) composed of a zinc finger array and the catalytic domain of the restriction endonuclease FokI. These ZF-nickases work as heterodimers in which one subunit has a catalytically inactive FokI domain. We present two different approaches to engineer highly specific nickases; both rely on the sequence-specific nicking activity of the DNA mismatch repair endonuclease MutH which we fused to a DNA-binding module, either a catalytically inactive variant of the homing endonuclease I-SceI or the DNA-binding domain of the TALE protein AvrBs4. The fusion proteins nick strand specifically a bipartite recognition sequence consisting of the MutH and the I-SceI or TALE recognition sequences, respectively, with a more than 1000-fold preference over a stand-alone MutH site. TALE-MutH is a programmable nickase.

  13. Label-free and ratiometric detection of nuclei acids based on graphene quantum dots utilizing cascade amplification by nicking endonuclease and catalytic G-quadruplex DNAzyme.

    PubMed

    Wang, Guang-Li; Fang, Xin; Wu, Xiu-Ming; Hu, Xue-Lian; Li, Zai-Jun

    2016-07-15

    Herein, we report a ratiometric fluorescence assay based on graphene quantum dots (GQDs) for the ultrasensitive DNA detection by coupling the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for cascade signal amplifications. With o-phenylenediamine acted as the substrate of G-quadruplex/hemin DNAzyme, whose oxidization product (that is, 2,3-diaminophenazine, DAP) quenched the fluorescence intensity of GQDs (at 460nm) obviously, accompanied with the emergence of a new emission of DAP (at 564nm). The ratiometric signal variations at the emission wavelengths of 564 and 460nm (I564/I460) were utilized for label-free, sensitive, and selective detection of target DNA. Utilizing the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for amplified cascade generation of DAP, the proposed bioassay exhibited high sensitivity toward target DNA with a detection limit of 30fM. The method also had additional advantages such as facile preparation and easy operation.

  14. On remembering and forgetting our autobiographical pasts: retrograde amnesia and Andrew Mayes's contribution to neuropsychological method.

    PubMed

    Kopelman, M D; Bright, P

    2012-11-01

    Andrew Mayes's contribution to the neuropsychology of memory has consisted in steadily teasing out the nature of the memory deficit in the amnesic syndrome. This has been done with careful attention to matters of method at all stages. This particularly applies to his investigations of forgetting rates in amnesia and to his studies of retrograde amnesia. Following a brief outline of his work, the main current theories of retrograde amnesia are considered: consolidation theory, episodic-to-semantic shift theory, and multiple trace theory. Findings across the main studies in Alzheimer dementia are reviewed to illustrate what appears to be consistently found, and what is much more inconsistent. A number of problems and issues in current theories are then highlighted--including the nature of the temporal gradient, correlations with the extent of temporal lobe damage, what we would expect 'normal' remote memory curves to look like, how they would appear in focal retrograde amnesia, and whether we can pinpoint retrograde amnesia to hippocampal/medial temporal damage on the basis of existing studies. A recent study of retrograde amnesia is re-analysed to demonstrate temporal gradients on recollected episodic memories in hippocampal/medial temporal patients. It is concluded that there are two requirements for better understanding of the nature of retrograde amnesia: (i) a tighter, Mayesian attention to method in terms of both the neuropsychology and neuroimaging in investigations of retrograde amnesia; and (ii) acknowledging that there may be multiple factors underlying a temporal gradient, and that episodic and semantic memory show important interdependencies at both encoding and retrieval. Such factors may be critical to understanding what is remembered and what is forgotten from our autobiographical pasts.

  15. Low-Frequency Response Following the Passage of Hurricane Andrew on the Texas-Louisiana Shelf

    NASA Astrophysics Data System (ADS)

    Pearce, S. M.; Smith, D. C.; Dimarco, S. F.

    2009-12-01

    During August 24th through 27th in 1992, Hurricane Andrew passed through the Gulf Of Mexico almost directly over several moorings on the easternmost Louisiana shelf portion of the Texas-Louisiana Shelf (LATEX) coastal ocean monitoring program. Examination of the current meter time-series showed the existence of fast moving, long shelf waves over the entire Texas-Louisiana shelf west of the storm passage for up to 12 days after direct forcing ceased. The LATEX program featured 31 moorings each with 3 current meters over the 10, 20, 50, and 200 meter isobaths in 5 cross sectional lines with additional coverage on the 200 meter isobath from the Louisiana-Mississippi River delta, to Corpus Christi, Texas. Additionally, several pressure records from LATEX and several NOAA historical coastal tide gauge data from Sabine Pass to Port Isabella, Texas were incorporated. Raw, 3-hour low pass filtered, and 40-hour low pass filtered versions of the current data were analyzed. The pressure data used were detided using a least squares fit, and the tidal records were detided using the NOAA predicted tides for that location. All data were analyzed using a wavelet analysis to determine the spectra over time. The analyzed data shows that the shelf response was largely dominated in the internal Kelvin wave mode. The wave propagated towards the west on the shelf at approximately 400 km/day. These results are contrasted and compared with wave modes predicted for coastal trapped wave solutions. The output of a coastal ocean model simulation using a forced wind field similar to the storm are also contrasted and compared with the observed data.

  16. Tests of executive functioning predict scores on the MacAndrew Alcoholism Scale.

    PubMed

    Deckel, A W

    1999-02-01

    1. Previous work reported that tests of executive functioning (EF) predict the risk of alcoholism in subject populations selected for a "high density" of a family history of alcoholism and/or the presence of sociopathic traits. The current experiment examined the ability of EF tests to predict the risk of alcoholism, as measured by the MacAndrew Alcoholism Scale (MAC), in outpatient subjects referred to a general neuropsychological testing service. 2. Sixty-eight male and female subjects referred for neuropsychological testing were assessed for their past drinking histories and administered the Wisconsin Card Sorting Test, the Wechsler Adult Intelligence Scale-Revised, the Trails (Part B) Test, and the MAC. Principal Components analysis (PCA) reduced the number of EF tests to two measures, including one that loaded on the WCST, and one that loaded on the Similarities, Picture Arrangement, and Trails tests. Multiple hierarchical regression first removed the variance from demographic variables, alcohol consumption, and verbal (i.e., Vocabulary) and non-verbal (i.e., Block Design) IQ, and then entered the executive functioning factors into the prediction of the MAC. 3. Seventy-six percent of the subjects were classified as either light, infrequent, or non-drinkers on the Quantity-Frequency-Variability scale. The factor derived from the WCST on PCA significantly added to the prediction of risk on the MAC (p = .0063), as did scores on Block Design (p = .033). Relatively more impaired scores on the WCST factor and Block Design were predictive of higher scores on the MAC. The other factors were not associated with MAC scores. 4. These results support the hypothesis that decrements in EF are associated with risk factors for alcoholism, even in populations where the density of alcoholic behaviors are not unusually high. When taken in conjunction with other findings, these results implicate EF test scores, and prefrontal brain functioning, in the neurobiology of the risk for

  17. Cylindric partitions, {{\\boldsymbol{ W }}}_{r} characters and the Andrews-Gordon-Bressoud identities

    NASA Astrophysics Data System (ADS)

    Foda, O.; Welsh, T. A.

    2016-04-01

    We study the Andrews-Gordon-Bressoud (AGB) generalisations of the Rogers-Ramanujan q-series identities in the context of cylindric partitions. We recall the definition of r-cylindric partitions, and provide a simple proof of Borodin’s product expression for their generating functions, that can be regarded as a limiting case of an unpublished proof by Krattenthaler. We also recall the relationships between the r-cylindric partition generating functions, the principal characters of {\\hat{{sl}}}r algebras, the {{\\boldsymbol{ M }}}r r,r+d minimal model characters of {{\\boldsymbol{ W }}}r algebras, and the r-string abaci generating functions, providing simple proofs for each. We then set r = 2, and use two-cylindric partitions to re-derive the AGB identities as follows. Firstly, we use Borodin’s product expression for the generating functions of the two-cylindric partitions with infinitely long parts, to obtain the product sides of the AGB identities, times a factor {(q;q)}∞ -1, which is the generating function of ordinary partitions. Next, we obtain a bijection from the two-cylindric partitions, via two-string abaci, into decorated versions of Bressoud’s restricted lattice paths. Extending Bressoud’s method of transforming between restricted paths that obey different restrictions, we obtain sum expressions with manifestly non-negative coefficients for the generating functions of the two-cylindric partitions which contains a factor {(q;q)}∞ -1. Equating the product and sum expressions of the same two-cylindric partitions, and canceling a factor of {(q;q)}∞ -1 on each side, we obtain the AGB identities.

  18. On remembering and forgetting our autobiographical pasts: retrograde amnesia and Andrew Mayes's contribution to neuropsychological method.

    PubMed

    Kopelman, M D; Bright, P

    2012-11-01

    Andrew Mayes's contribution to the neuropsychology of memory has consisted in steadily teasing out the nature of the memory deficit in the amnesic syndrome. This has been done with careful attention to matters of method at all stages. This particularly applies to his investigations of forgetting rates in amnesia and to his studies of retrograde amnesia. Following a brief outline of his work, the main current theories of retrograde amnesia are considered: consolidation theory, episodic-to-semantic shift theory, and multiple trace theory. Findings across the main studies in Alzheimer dementia are reviewed to illustrate what appears to be consistently found, and what is much more inconsistent. A number of problems and issues in current theories are then highlighted--including the nature of the temporal gradient, correlations with the extent of temporal lobe damage, what we would expect 'normal' remote memory curves to look like, how they would appear in focal retrograde amnesia, and whether we can pinpoint retrograde amnesia to hippocampal/medial temporal damage on the basis of existing studies. A recent study of retrograde amnesia is re-analysed to demonstrate temporal gradients on recollected episodic memories in hippocampal/medial temporal patients. It is concluded that there are two requirements for better understanding of the nature of retrograde amnesia: (i) a tighter, Mayesian attention to method in terms of both the neuropsychology and neuroimaging in investigations of retrograde amnesia; and (ii) acknowledging that there may be multiple factors underlying a temporal gradient, and that episodic and semantic memory show important interdependencies at both encoding and retrieval. Such factors may be critical to understanding what is remembered and what is forgotten from our autobiographical pasts. PMID:22884958

  19. Damage to unburied flowlines in the Gulf of Mexico during Hurricane Andrew

    SciTech Connect

    Collins, J.I.

    1995-12-31

    The US Minerals Management Service reported that 454 sub-sea pipelines were damaged during Hurricane Andrew. Previously, damage to pipelines and flowlines has been reported in a series of papers by Blumberg (1964). In the present paper a formulation of the hydrodynamic loads acting on a flowline which is lying on the seafloor in the presence of waves and currents is summarized. In general, the line dynamics can be represented by a fourth order differential equation with nonlinear forcing but a method is presented which assumes that the complete response can be broken down into distinct phases from ``early motion`` to ``taut line`` to ``yield and breaking``. The selection of appropriate force coefficients and boundary layer interactions is discussed. The initial stages of pipeline migration across the seafloor is shown to be followed by loading of the flowline once all of the ``slack`` is taken up. The loads are shown to be a function of the current orientation and the maximum tension is approximately proportional to the square of the distance between risers. Comparisons of predicted and observed damages confirm that ``effective`` boundary layer thicknesses at the seafloor and typical drag and lift coefficients which were selected based on available literature are consistent with observed flowline damage. The results of sidescan sonar mosaics of pipeline migrations and reported damage are consistent with the loads predicted by relatively simple hydrodynamic/structural models. The material presented in this paper permits the evaluation of the risk of damage to unburied flowlines using relatively simple tools. Guidelines to the assumptions of force coefficients and flowline responses are provided. 20 refs., 11 figs., 2 tabs.

  20. A tale of two storms: Surges and sediment deposition from Hurricanes Andrew and Wilma in Florida’s southwest coast mangrove forests: Chapter 6G in Science and the storms-the USGS response to the hurricanes of 2005

    USGS Publications Warehouse

    Smith, Thomas J.; Anderson, Gordon H.; Tiling, Ginger

    2007-01-01

    Hurricanes can be very different from each other. Here we examine the impacts that two hurricanes, Andrew and Wilma, had in terms of storm surge and sediment deposition on the southwest coast of Florida. Although Wilma was the weaker storm, it had the greater impact. Wilma had the higher storm surge over a larger area and deposited more sediment than did Andrew. This effect was most likely due to the size of Wilma's eye, which was four times larger than that of Andrew.

  1. Effect of Hurricane Andrew on the Turkey Point Nuclear Generating Station from August 20--30, 1992. [Final report

    SciTech Connect

    Hebdon, F.J.

    1993-03-01

    On August 24, 1992, Hurricane Andrew, a Category 4 hurricane, struck the Turkey Point Electrical Generating Station with sustained winds of 145 mph (233 km/h). This is the report of the team that the US Nuclear Regulatory Commission (NRC) and the Institute of Nuclear Power Operations (INPO) jointly sponsored (1) to review the damage that the hurricane caused the nuclear units and the utility`s actions to prepare for the storm and recover from it, and (2) to compile lessons that might benefit other nuclear reactor facilities.

  2. Medical societies and insanity in late-eighteenth-century London: the fight between Andrew Marshal and John Hunter.

    PubMed

    Wade, Nicholas J

    2005-03-01

    In 1789, at a meeting of a small London society for the promotion of medical and surgical knowledge, Andrew Marshal (1742-1813) and John Hunter (1728-1793) engaged in heated debate regarding the association between mania and the structure of the brain. Marshal claimed to have observed abnormalities when dissecting brains of those who died insane and Hunter denied this connection. At the next meeting a scuffle between them ensued and they had to be parted. Although Marshal did not publish his observations during his lifetime, they were assembled by his assistant in 1815. Marshal's descriptions of the brains of hydrophobics and maniacs are worthy of note.

  3. The psychological effects of Hurricane Andrew on ethnic minority and Caucasian children and adolescents: a case study.

    PubMed

    Jones, R T; Frary, R; Cunningham, P; Weddle, J D; Kaiser, L

    2001-02-01

    The impact of Hurricane Andrew on 212 African American, Caucasian, and Hispanic elementary and middle school children was examined at 6 months postdisaster. Using self-report instruments, this case study examined the predictive utility of several hypothesized mediators of children's reactions to disaster. Results showed higher levels of intrusive symptomatology for girls and for elementary school children as compared with their middle school counterparts. No differences were found with reference to race. The lack of findings concerning race is addressed, as well as implications for future studies.

  4. Evaluation of Hydraulically Significant Discontinuities in Dockum Group Mudrocks in Andrews County, Texas

    NASA Astrophysics Data System (ADS)

    Holt, R. M.; Kuszmaul, J. S.; Cao, S.; Powers, D. W.

    2013-12-01

    Triassic mudrocks of the Dockum Group (Cooper Canyon Formation) host four, below-grade landfills at the Waste Control Specialists (WSC) site in Andrews County, Texas, including: a hazardous waste landfill and three radioactive waste landfills. At the study site, the Dockum consists of mudrocks with sparse siltstone/sandstone interbeds that developed in a semi-arid environment from an ephemeral meandering fluvial system. Sedimentary studies reveal that the mudrocks are ancient floodplain vertisols (soils with swelling clays) and siltstone/sandstone interbeds are fluvial channel deposits that were frequently subaerially exposed. Rock discontinuities, including fractures and syndepositional slickensided surfaces, were mapped during the excavation of the WCS radioactive waste landfills along vertical faces prepared by the construction contractor. Face locations were selected to insure a sampled area with nearly complete vertical coverage for each landfill. Individual discontinuities were mapped and their strike, dip, length, roughness, curvature, staining, and evidence of displacement were described. In the three radioactive waste disposal landfills, over 1750 discontinuities across 35 excavated faces were mapped and described, where each face was nominally 8 to 10 ft tall and 50 to 100 ft long. Genetic units related to paleosol development were identified. On average, the orientation of the discontinuities was horizontal, and no other significant trends were observed. Mapping within the landfill excavations shows that most discontinuities within Dockum rocks are horizontal, concave upward, slickensided surfaces that developed in the depositional environment, as repeated wetting and drying cycles led to shrinking and swelling of floodplain vertisols. Fractures that showed staining (a possible indicator of past or present hydraulic activity) are rare, vertical to near-vertical, and occur mainly in, and adjacent to, mechanically stiff siltstone and sandstone interbeds

  5. Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.

    PubMed

    Loebel, D A; Johnston, P G

    1993-01-01

    The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X chromosomes to be distinguished. No difference was found between the active and inactive X chromosomes with DNase I or MspI digestion. When chromosomes were digested with the methylation sensitive restriction enzymes HpaII and HhaI, the inactive X chromosome was labelled to a greater extent. These results indicate no overall difference in chromatin condensation between the active and inactive X chromosomes and greater overall methylation of the active X chromosome. This relative undermethylation of the inactive X chromosome may be important in X chromosome inactivation, but its function, if any, remains to be determined. PMID:8381740

  6. Mechanism of action of nalidixic acid: Purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme

    PubMed Central

    Sugino, Akio; Peebles, Craig L.; Kreuzer, Kenneth N.; Cozzarelli, Nicholas R.

    1977-01-01

    A target protein for nalidixic and oxolinic acids in Escherichia coli, the nalA gene product (Pnal), was purified to homogeneity as judged by gel electrophoresis, using an in vitro complementation assay. It is a dimer of identical 110,000-dalton subunits. A polypeptide of this molecular weight is uniquely induced by a λ nalA transducing phage, thereby showing that the purified Pnal is a product of the nalA gene. Nalidixic and oxolinic acids inhibit DNA gyrase activity and induce formation of a relaxation complex analogue. Treatment of the complex with sodium dodecyl sulfate causes a doublestrand break in the DNA substrate and the resulting linear molecule seems covalently bound to protein. Complex formation, unlike the introduction of supertwists, does not require ATP or relaxed circular DNA and is insensitive to novobiocin. DNA gyrase from a strain with a nalA mutation conferring drug resistance (nalAr) is 1/100 as sensitive to oxolinic and nalidixic acids with respect to inhibition of supertwisting and induction of the pre-linearization complex. Addition of Pnal restores drug sensitivity and stimulates DNA gyrase activity. DNA gyrase preparations and Pnal catalyze a third reaction sensitive to nalidixic and oxolinic acids, the ATP-independent relaxation of supertwister DNA. Relaxation by gyrase from nalAr cells is drug resistant. The nicking-closing activity is distinct from E. coli ω protein in several properties, including the ability to relax positively supertwisted DNA. We postulate that the nalA gene product occurs in two molecular forms, as Pnal and as a gyrase component. Both forms catalyze nicking-closing, and inhibition of this activity by nalidixic and oxolinic acids may account for the inhibition of DNA synthesis by these drugs. Images PMID:200930

  7. Sensitive detection of microRNA in complex biological samples via enzymatic signal amplification using DNA polymerase coupled with nicking endonuclease.

    PubMed

    Yin, Bin-Cheng; Liu, Yu-Qiang; Ye, Bang-Ce

    2013-12-01

    MicroRNA (miRNA) has become an ideal biomarker candidate for cancer diagnosis, prognosis, and therapy. In this study, we have developed a novel one-step method for sensitive and specific miRNA detection via enzymatic signal amplification and demonstrated its practical application in biological samples. The proposed signal amplification strategy is an integrated "biological circuit" designed to initiate a cascade of enzymatic polymerization reactions in order to detect, amplify, and measure a specific miRNA sequence by using the isothermal strand-displacement property of a mesophilic DNA polymerase together with the nicking activity of a restriction endonuclease. The circuit is composed of two molecular switches operating in series: the nicking endonuclease-assisted isothermal polymerization reaction activated by a specific miRNA and the strand-displacement polymerization reaction designed to initiate molecular beacon-assisted amplification and signal transduction. The hsa-miR-141 (miR-141) was chosen as a target miRNA because its level specifically elevates in prostate cancer. The proposed method allowed quantitative sequence-specific detection of miR-141 in a dynamic range from 1 fM to 100 nM, with an excellent ability to discriminate differences among miR-200 family members. Moreover, the detection assay was applied to quantify miR-141 in cancerous cell lysates. The results are in excellent agreement with those from the reverse transcription polymerase chain reaction method. On the basis of these findings, we believe that this proposed sensitive and specific assay has great potential as a miRNA quantification method for use in biomedical research and clinical diagnosis.

  8. Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signal amplification.

    PubMed

    Zhao, Yongxi; Chen, Feng; Wu, Yayan; Dong, Yanhua; Fan, Chunhai

    2013-04-15

    Herein, using DNA adenine methylation (Dam) methyltransferase (MTase) as a model analyte, a simple, rapid, and highly sensitive fluorescence sensing platform for monitoring the activity and inhibition of DNA MTase was developed on the basis of methylation-sensitive cleavage and nicking enzyme-assisted signal amplification. In the presence of Dam MTase, an elaborately designed hairpin probe was methylated. With the help of methylation-sensitive restriction endonuclease DpnI, the methylated hairpin probe could be cleaved to release a single-stranded DNA (ssDNA). Subsequently, this released ssDNA would hybridize with the molecular beacon (MB) to open its hairpin structure, resulting in the restoration of fluorescence signal as well as formation of the double-stranded recognition site for nicking enzyme Nt.BbvCI. Eventually, an amplified fluorescence signal was observed through the enzymatic recycling cleavage of MBs. Based on this unique strategy, a very low detection limit down to 0.06 U/mL was achieved within a short assay time (60 min) in one step, which is superior to those of most existing approaches. Owing to the specific site recognition of MTase toward its substrate, the proposed sensing system was able to readily discriminate Dam MTase from other MTase such as M.SssI and even detect the target in complex biological matrix. Furthermore, the application of the proposed sensing strategy for screening Dam MTase inhibitors was also demonstrated with satisfactory results. This novel method not only provides a promising platform for monitoring activity and inhibition of DNA MTases, but also shows great potentials in biological process researches, drugs discovery and clinical diagnostics.

  9. Mangroves, hurricanes, and lightning strikes: Assessment of Hurricane Andrew suggests an interaction across two differing scales of disturbance

    USGS Publications Warehouse

    Smith, Thomas J.; Robblee, Michael B.; Wanless, Harold R.; Doyle, Thomas W.

    1994-01-01

    The track of Hurricane Andrew carried it across one of the most extensive mangrove for ests in the New World. Although it is well known that hurricanes affect mangrove forests, surprisingly little quantitative information exists concerning hurricane impact on forest structure, succession, species composition, and dynamics of mangrove-dependent fauna or on rates of eco-system recovery (see Craighead and Gilbert 1962, Roth 1992, Smith 1992, Smith and Duke 1987, Stoddart 1969).After Hurricane Andrew's passage across south Florida, we assessed the environmental damage to the natural resources of the Everglades and Biscayne National Parks. Quantitative data collected during subsequent field trips (October 1992 to July 1993) are also provided. We present measurements of initial tree mortality by species and size class, estimates of delayed (or continuing) tree mortality, and observations of geomorphological changes along the coast and in the forests that could influence the course of forest recovery. We discuss a potential interaction across two differing scales of disturbance within mangrove forest systems: hurricanes and lightning strikes.

  10. Evaluation of long-term community recovery from Hurricane Andrew: sources of assistance received by population sub-groups.

    PubMed

    McDonnell, S; Troiano, R P; Barker, N; Noji, E; Hlady, W G; Hopkins, R

    1995-12-01

    Two three-stage cluster surveys were conducted in South Dade County, Florida, 14 months apart, to assess recovery following Hurricane Andrew. Response rates were 75 per cent and 84 per cent. Sources of assistance used in recovery from Hurricane Andrew differed according to race, per capita income, ethnicity, and education. Reports of improved living situation post-hurricane were not associated with receiving relief assistance, but reports of a worse situation were associated with loss of income, being exploited, or job loss. The number of households reporting problems with crime and community violence doubled between the two surveys. Disaster relief efforts had less impact on subjective long-term recovery than did job or income loss or housing repair difficulties. Existing sources of assistance were used more often than specific post-hurricane relief resources. The demographic make-up of a community may determine which are the most effective means to inform them after a disaster and what sources of assistance may be useful.

  11. Patterns and drivers of Holocene vegetational change near the prairie-forest ecotone in Minnesota: revisiting McAndrews' transect.

    PubMed

    Nelson, David M; Hu, Feng Sheng

    2008-07-01

    Holocene vegetational dynamics along the prairie-forest border of Minnesota were first documented in McAndrews' classic work. Despite numerous subsequent paleo-studies, a number of questions remain unanswered about the vegetation history of the region. Here, pollen, stable-isotope, mineral, and charcoal data are described from three lakes near McAndrews' sites. These data were compared with other paleoenvironmental records to reconstruct vegetation, aridity, and fire. The climate was relatively wet with increasing summer temperatures before approximately 8000 yr before present (BP). The rates of changes were asymmetric for the onset and termination of middle-Holocene aridity, with an abrupt increase at approximately 8000 yr BP and a gradual, but variable, decline from approximately 7800 to 4000 yr BP. Early-Holocene coniferous forests changed to mixed-grass prairie without an intervening period of tallgrass prairie or deciduous forest, whereas the retreat of prairie was characterized by transitions from mixed-grass to tallgrass prairie to deciduous forest and finally to coniferous forest. Within the middle Holocene, the composition and structures of grass-dominated vegetation varied both temporally and spatially. Fire primarily responded to changes in climate and fuel loads. Vegetation was more strongly influenced by climatic changes than by fire-regime shifts.

  12. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas is... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base,...

  13. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  14. The brain and the biology of belief: An interview with Andrew Newberg, MD. Interview by Nancy Nachman-Hunt.

    PubMed

    Newberg, Andrew

    2009-01-01

    Andrew Newberg, MD, is an associate professor in the Department of Radiology, Division of Nuclear Medicine, at the Hospital of the University of Pennsylvania, Philadelphia, with secondary appointments in the Departments of Psychiatry and Religious Studies. He is actively involved in neuroimaging research projects, including the study of the neurophysiological correlates of meditation and other types of complementary therapies. Dr Newberg's research now largely focuses on how brain function is associated with various mental states, in particular, the relationship between brain function and mystical or religious experiences. He has authored several books, including Why God Won't Go Away: Brain Science and the Biology of Belief (Ballantine/Random House, 2001) and coauthor with Eugene G. d'Aquili, MD, of The Mystical Mind: Probing the Biology of Religious Experience (Fortress Press, 1999). His most recent book is How God Changes Your Brain, with coauthor Mark Waldman (Ballantine Books, 2009).

  15. Plant phenology patterns at three sites on the H.J. Andrews Experimental Forest, Oregon, 1987 to 2007.

    NASA Astrophysics Data System (ADS)

    Shaw, D. C.; Creel, C.; Downing, G.; Remillard, S.; O'Connell, K.

    2007-12-01

    Plant phenology data has been collected at three sites on the H.J. Andrews Experimental Forest, Oregon since the late 1970's. The sites were visited once every three weeks year-round. Current efforts to clean and archive this data are on-going. Here we present on a 20 year data set from 1987 to 2007. The three sites are located at Watersheds (WS) 10, 8, and 7/6, at an elevation of 466 m, 993 m, and 905/950 m respectively. Forests were old growth (WS 8) and regenerating clearcuts (WS 6, 10), or shelterwood clearcut with overstory removed in 1984 (WS 7) dominated by Douglas-fir, western hemlock, true firs, and western redcedar. One tree (Douglas-fir), two evergreen shrubs, three deciduous shrubs, and four herbs were followed for vegetative and flowering phenology, including bud swell, bud bread, leaf expansion, leaf color change, leaf fall, flower bud swell, blooming, petal loss, fruit formation, and seed dispersal. Weather stations are located at each site and tied into a network of stations in this LTER site. Physical factors such as snow depth, snow coverage, freeze-thaw activity, as well as lichen condition were also noted. We are asking two key questions regarding plant phenology patterns. 1. Has the growing season lengthened in the mountainous watershed of the HJ Andrews, and is this similar for a low elevation site (WS 10) versus a mid elevation site (WS 8, 7/6)? 2. Do snow-pack dynamics influence plant phenology more so than temperature (degree days)?

  16. Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform.

    PubMed

    Li, Xiang; Ding, Xuelian; Fan, Jing

    2015-12-01

    Sensitive and selective detection of ultralow concentrations of specific biomolecules is important in early clinical diagnoses and biomedical applications. Many types of aptasensors have been developed for the detection of various biomolecules, but usually suffer from false positive signals and high background signals. In this work, we have developed an amplified fluorescence aptasensor platform for ultrasensitive biomolecule detection based on enzyme-assisted target-recycling signal amplification and graphene oxide. By using a split molecular aptamer beacon and a nicking enzyme, the typical problem of false positive signals can be effectively resolved. Only in the presence of a target biomolecule, the sensor system is able to generate a positive signal, which significantly improves the selectivity of the aptasensor. Moreover, using graphene oxide as a super-quencher can effectively reduce the high background signal of a sensing platform. We select vascular endothelial growth factor (VEGF) and adenosine triphosphate (ATP) as model analytes in the current proof-of-concept experiments. It is shown that under optimized conditions, our strategy exhibits high sensitivity and selectivity for the quantification of VEGF and ATP with a low detection limit (1 pM and 4 nM, respectively). In addition, this biosensor has been successfully utilized in the analysis of real biological samples.

  17. Ab initio DNA synthesis by Bst polymerase in the presence of nicking endonucleases Nt.AlwI, Nb.BbvCI, and Nb.BsmI.

    PubMed

    Antipova, Valeriya N; Zheleznaya, Lyudmila A; Zyrina, Nadezhda V

    2014-08-01

    In the absence of added DNA, thermophilic DNA polymerases synthesize double-stranded DNA from free dNTPs, which consist of numerous repetitive units (ab initio DNA synthesis). The addition of thermophilic restriction endonuclease (REase), or nicking endonuclease (NEase), effectively stimulates ab initio DNA synthesis and determines the nucleotide sequence of reaction products. We have found that NEases Nt.AlwI, Nb.BbvCI, and Nb.BsmI with non-palindromic recognition sites stimulate the synthesis of sequences organized mainly as palindromes. Moreover, the nucleotide sequence of the palindromes appeared to be dependent on NEase recognition/cleavage modes. Thus, the heterodimeric Nb.BbvCI stimulated the synthesis of palindromes composed of two recognition sites of this NEase, which were separated by AT-reach sequences or (A)n (T)m spacers. Palindromic DNA sequences obtained in the ab initio DNA synthesis with the monomeric NEases Nb.BsmI and Nt.AlwI contained, along with the sites of these NEases, randomly synthesized sequences consisted of blocks of short repeats. These findings could help investigation of the potential abilities of highly productive ab initio DNA synthesis for the creation of DNA molecules with desirable sequence.

  18. A Novel Ultrasensitive ECL Sensor for DNA Detection Based on Nicking Endonuclease-Assisted Target Recycling Amplification, Rolling Circle Amplification and Hemin/G-Quadruplex

    PubMed Central

    Luo, Fukang; Xiang, Guimin; Pu, Xiaoyun; Yu, Juanchun; Chen, Ming; Chen, Guohui

    2015-01-01

    In this study, we describe a novel universal and highly sensitive strategy for the electrochemiluminescent (ECL) detection of sequence specific DNA at the aM level based on Nt.BbvCI (a nicking endonuclease)-assisted target recycling amplification (TRA), rolling circle amplification (RCA) and hemin/G-quadruplex. The target DNAs can hybridize with self-assembled capture probes and assistant probes to form “Y” junction structures on the electrode surface, thus triggering the execution of a TRA reaction with the aid of Nt.BbvCI. Then, the RCA reaction and the addition of hemin result in the production of numerous hemin/G-quadruplex, which consume the dissolved oxygen in the detection buffer and result in a significant ECL quenching effect toward the O2/S2O82− system. The proposed strategy combines the amplification ability of TRA, RCA and the inherent high sensitivity of the ECL technique, thus enabling low aM (3.8 aM) detection for sequence-specific DNA and a wide linear range from 10.0 aM to 1.0 pM. At the same time, this novel strategy shows high selectivity against single-base mismatch sequences, which makes our novel universal and highly sensitive method a powerful addition to specific DNA sequence detection. PMID:25629701

  19. Sensitive detection of T4 polynucleotide kinase activity based on coupled exonuclease reaction and nicking enzyme-assisted fluorescence signal amplification.

    PubMed

    Hou, Ting; Wang, Xiuzhong; Lu, Tingting; Liu, Xiaojuan; Li, Feng

    2014-05-01

    As a prominent member of the 5'-kinase family, T4 polynucleotide kinase (PNK) plays an important role in gene function regulations, and the study of PNK activity and its potential inhibitors is significant for research related to the DNA phosphorylation process. Here, we proposed a novel strategy for the detection of PNK activity and its inhibition, which combines exonuclease enzyme reaction and nicking enzyme-assisted fluorescence signal amplification. Through recycling cleavage of DNA fluorescence probe for signal amplification, a highly sensitive PNK sensing platform is developed, and a very low detection limit of 0.05 mU/mL is achieved, which is better than or comparable to that of the previously reported PNK assays. The present approach adopts a simple separation-free procedure in which the enzyme assay is conducted in homogeneous solutions. Additionally, the inhibitory effects of several known kinase inhibitors on PNK have been successfully detected. Since the proposed assay exhibits the advantages of high sensitivity and simplicity, it holds great potential in providing a promising platform for convenient and highly sensitive detection of PNK activity and its inhibitors.

  20. Once a physicist: Nick Horvath

    NASA Astrophysics Data System (ADS)

    Horvath, Nick

    2009-05-01

    How did you get into basketball? That's an easy one: I was tall. I had zero interest in sport at a young age and was pushed into basketball against my will when I was about eight. I hated it. The next year, however, I was so much taller than everyone else that in my first game, even though I had no idea what I was doing, I could simply hold the ball above my head and shoot over everyone. I fell in love with basketball after that first game. If I had played badly that day, it is likely that I would not have played again in my entire life.

  1. TRIGGER SELF-CONTROL AND SEIZURE ARREST IN THE ANDREWS/REITER BEHAVIORAL APPROACH TO EPILEPSY: A RETROSPECTIVE ANALYSIS OF SEIZURE FREQUENCY

    PubMed Central

    Michaelis, Rosa; Schonfeld, Warren; Elsas, Siegward-M.

    2012-01-01

    The aim of this retrospective study is to describe changes of seizure frequency in epilepsy patients who participated in the Andrews/Reiter behavioral intervention for epilepsy. For this uncontrolled retrospective study, data was extracted from patients’ medical journals. Intention-to-treat-analyses were restricted to patients with sufficient documentation supporting a diagnosis of probable or definite epilepsy. Main outcome variable was a comparison of mean seizure frequency at baseline and towards completion of the program. The seizure frequency of 30 (50%%) patients showed a clinically meaningful improvement (>50% reduction of seizures) towards the end of the intervention. Twenty-two (37%) patients became seizure-free at the end of the intervention. In summary, a clinically meaningful reduction in reported seizure frequency was observed in epilepsy patients who received the Andrews/Reiter intervention for epilepsy. Prospective trials are needed to further investigate the program’s efficacy and to study epileptic seizure triggers. PMID:22341960

  2. Effects of 3′-OH and 5′-PO4 Base Mispairs and Damaged Base Lesions on the Fidelity of Nick Sealing by Deinococcus radiodurans RNA Ligase

    PubMed Central

    Schmier, Brad J.

    2014-01-01

    Deinococcus radiodurans RNA ligase (DraRnl) is the founding member of a family of end-joining enzymes encoded by diverse microbes and viruses. DraRnl ligates 3′-OH, 5′-PO4 nicks in double-stranded nucleic acids in which the nick 3′-OH end is RNA. Here we gauge the effects of 3′-OH and 5′-PO4 base mispairs and damaged base lesions on the rate of nick sealing. DraRnl is indifferent to the identity of the 3′-OH nucleobase, provided that it is correctly paired. With 3′-OH mispairs, the DraRnl sealing rate varies widely, with G-T and A-C mispairs being the best substrates and G-G, G-A, and A-A mispairs being the worst. DraRnl accepts 3′ A–8-oxoguanine (oxoG) to be correctly paired, while it discriminates against U-oxoG and G-oxoG mispairs. DraRnl displays high activity and low fidelity in sealing 3′-OH ends opposite an 8-oxoadenine lesion. It prefers 3′-OH adenosine when sealing opposite an abasic template site. With 5′-PO4 mispairs, DraRnl seals a 5′ T-G mispair as well as it does a 5′ C-G pair; in most other respects, the ligation fidelity at 5′ mispairs is similar to that at 3′ mispairs. DraRnl accepts a 5′ A-oxoG end to be correctly paired, yet it is more tolerant of 5′ T-oxoG and 5′ G-oxoG mispairs than the equivalent configurations on the 3′ side of the nick. At 5′ nucleobase-abasic site nicks, DraRnl prefers to ligate when the nucleobase is a purine. The biochemical properties of DraRnl are compatible with its participation in the templated repair of RNA damage or in the sealing of filled DNA gaps that have a 3′ ribopatch. PMID:24532777

  3. Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers.

    PubMed

    Sreedhar, Reddampalli V; Venkatachalam, Lakshmanan; Bhagyalakshmi, Neelwarne

    2007-08-01

    Occurrence of genetic variants during micropropagation is occasionally encountered when the cultures are maintained in vitro for long period. Therefore, the micropropagated multiple shoots of Vanilla planifolia Andrews developed from axillary bud explants established 10 years ago were used to determine somaclonal variation using random amplified polymorphic DNA (RAPD) and intersimple sequence repeats markers (ISSR). One thousand micro-plants were established in soil of which 95 plantlets (consisting of four phenotypes) along with the mother plant were subjected to genetic analyses using RAPD and ISSR markers. Out of the 45 RAPD and 20 ISSR primers screened, 30 RAPD and 7 ISSR primers showed 317 clear, distinct and reproducible band classes resulting in a total of 30 115 bands. However, no difference was observed in banding patterns of any of the samples for a particular primer, indicating the absence of variation among the micropropagated plants. Our results allow us to conclude that the micropropagation protocol that we have used for in vitro proliferation of vanilla plantlets for the last 10 years might be applicable for the production of clonal plants over a considerable period of time.

  4. Lessons learned from Hurricane Andrew: recommendations for care of the elderly in long-term care facilities.

    PubMed

    Silverman, M A; Weston, M; Llorente, M; Beber, C; Tam, R

    1995-06-01

    We report on the experience of a 500-bed, long-term care facility in Miami, Fla, which provides housing and nursing care units for patients--ranging from those who are independently ambulatory to those who are acutely ill and feeble--in preparing for, during, and in the immediate aftermath of Hurricane Andrew, which struck on August 24, 1992. The problems encountered included a massive influx of evacuated elderly to the facility, facility isolation, loss of electrical power, loss of running water, special dietary needs, and limited professional staffing due to personal property losses or loss of transportation. Overwhelmed county emergency medical services, limited access to hospitals and patient care, and difficulty in procuring supplies exacerbated the already complicated situation resulting from the storm. As a result of these catastrophic conditions, a number of challenges specific to the care of the elderly were identified. In conjunction with the Florida Department of Elder Affairs, we drafted a comprehensive blueprint that could serve as a disaster plan for other long-term care facilities facing a similar threat during the hurricane season.

  5. Nomina nova in Platyhelminthes pro Macrorhynchus von Graff, 1882 (non [Gmelin, 1801]; non Dunker, 1843), and Leptocleidus Mueller, 1936 (non Andrews, 1922).

    PubMed

    Hornung, Jahn J

    2016-08-19

    Two genus-group names of flat-worms-Leptocleidus Mueller, 1936 and Macrorhynchus von Graff, 1882-are junior homonyms that are preoccupied by fossil diapsid reptile genera-Leptocleidus Andrews, 1922, and Macrorhynchus Dunker, 1843-and an extant teleost fish genus-Macrorhynchus [Gmelin, 1801] ex La Cépède, 1800. These are replaced by nomina nova (Pharyngodytes nom. nov.; Graffiellus nom. nov.). Macrorhynchus [Gmelin, 1801] is an objective senior synonym of Macrorhyncus Dumeríl, 1805 ex La Cépède, 1800 (syn. nov.), and a senior homonym of Macrorhynchus Dunker, 1843, and Macrorhynchus von Graff, 1882.

  6. Nomina nova in Platyhelminthes pro Macrorhynchus von Graff, 1882 (non [Gmelin, 1801]; non Dunker, 1843), and Leptocleidus Mueller, 1936 (non Andrews, 1922).

    PubMed

    Hornung, Jahn J

    2016-01-01

    Two genus-group names of flat-worms-Leptocleidus Mueller, 1936 and Macrorhynchus von Graff, 1882-are junior homonyms that are preoccupied by fossil diapsid reptile genera-Leptocleidus Andrews, 1922, and Macrorhynchus Dunker, 1843-and an extant teleost fish genus-Macrorhynchus [Gmelin, 1801] ex La Cépède, 1800. These are replaced by nomina nova (Pharyngodytes nom. nov.; Graffiellus nom. nov.). Macrorhynchus [Gmelin, 1801] is an objective senior synonym of Macrorhyncus Dumeríl, 1805 ex La Cépède, 1800 (syn. nov.), and a senior homonym of Macrorhynchus Dunker, 1843, and Macrorhynchus von Graff, 1882. PMID:27615836

  7. An Analysis and Comparison of Two Short Writings: "Inaugural Address at the University of St. Andrew's" by J.S. Mill and "The University of Utopia" by R.M. Hutchins, Based on Five Criteria.

    ERIC Educational Resources Information Center

    Poirier, Jeannine M.

    Focusing on the concept of education for work vs. education for living, the author presents a comparative analysis of two works on liberal education, each of which was originally delivered orally to university students: "The Inaugural Address at the University of St. Andrew" by John Stuart Mill and "The University of Utopia" by Robert Maynard…

  8. Book Review: Maxwell's Demon 2: Entropy, classical and quantum information, computing. Harvey Leff and Andrew Rex (Eds.); Institute of Physics, Bristol, 2003, 500pp., US 55, ISBN 0750307595

    NASA Astrophysics Data System (ADS)

    Shenker, Orly R.

    2004-09-01

    In 1867, James Clerk Maxwell proposed a perpetuum mobile of the second kind, that is, a counter example for the Second Law of thermodynamics, which came to be known as "Maxwell's Demon." Unlike any other perpetual motion machine, this one escaped attempts by the best scientists and philosophers to show that the Second Law or its statistical mechanical counterparts are universal after all. "Maxwell's demon lives on. After more than 130 years of uncertain life and at least two pronouncements of death, this fanciful character seems more vibrant than ever." These words of Harvey Leff and Andrew Rex (1990), which open their introduction to Maxwell's Demon 2: Entropy, Classical and Quantum Information, Computing (hereafter MD2) are very true: the Demon is as challenging and as intriguing as ever, and forces us to think and rethink about the foundations of thermodynamics and of statistical mechanics.

  9. The impact of Hurricane Andrew on deviant behavior among a multi-racial/ethnic sample of adolescents in Dade County, Florida: a longitudinal analysis.

    PubMed

    Khoury, E L; Warheit, G J; Hargrove, M C; Zimmerman, R S; Vega, W A; Gil, A G

    1997-01-01

    Findings from a longitudinal study are presented on the relationships between the problems and stresses resulting from Hurricane Andrew and posthurricane minor deviant behavior. The sample (N = 4,978) included Hispanic, African-American, and White non-Hispanic middle school students enrolled in Dade County, Florida public schools. Two waves of data were collected prior to the hurricane; a third was obtained approximately 6 months following the storm. Results indicated that females were likely to report higher levels of hurricane-related stress symptoms than males. After controlling for prehurricane levels of minor deviance, family support, and race/ethnicity, hurricane stress symptom level remained a significant predictor of posthurricane minor deviant behavior. The findings lend support to stress theories of social deviance. PMID:9018678

  10. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    ScienceCinema

    Moniz, Ernest [U.S. Energy Secretary

    2016-07-12

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  11. Hurricane Frederic tidal floods of September 12-13, 1979, along the Gulf Coast, Pine Beach, St. Andrews Bay and Fort Morgan quadrangles, Alabama

    USGS Publications Warehouse

    Scott, John C.; Bohman, Larry R.

    1980-01-01

    Shown on a topographic map are floodmark elevations and approximate areas flooded by Hurricane Frederic tides of September 12-13, 1979, along the shores of St. Andrews Bay, Mobile Bay, and Bon Secour Bay from Fort Morgan eastward to about four miles east of Gasque, Ala. The storm tide went completely across the land between the beach and Mobile Bay throughout much of the area. Most homes on the beach side of Alabama State Highway 180 were completely destroyed, and the highway was washed out in several places. Damage to homes and other structures on the bay side was not as great. Storm-tide frequency and records of annual maximum tides at Mobile, Ala., since 1772, are presented. Offshore winds reached about 160 miles per hour. A wind-velocity of about 145 miles per hour was recorded near Dauphin Island, Ala. (USGS)

  12. The impact of Hurricane Andrew on deviant behavior among a multi-racial/ethnic sample of adolescents in Dade County, Florida: a longitudinal analysis.

    PubMed

    Khoury, E L; Warheit, G J; Hargrove, M C; Zimmerman, R S; Vega, W A; Gil, A G

    1997-01-01

    Findings from a longitudinal study are presented on the relationships between the problems and stresses resulting from Hurricane Andrew and posthurricane minor deviant behavior. The sample (N = 4,978) included Hispanic, African-American, and White non-Hispanic middle school students enrolled in Dade County, Florida public schools. Two waves of data were collected prior to the hurricane; a third was obtained approximately 6 months following the storm. Results indicated that females were likely to report higher levels of hurricane-related stress symptoms than males. After controlling for prehurricane levels of minor deviance, family support, and race/ethnicity, hurricane stress symptom level remained a significant predictor of posthurricane minor deviant behavior. The findings lend support to stress theories of social deviance.

  13. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    SciTech Connect

    Moniz, Ernest

    2014-02-03

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  14. The effects of benzene exposure on apoptosis in epithelial lung cells: localization by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and the immunocytochemical localization of apoptosis-related gene products.

    PubMed

    Weaver, C V; Liu, S-P; Lu, J-F; Lin, B-S

    2007-05-01

    Although benzene, a well-known human carcinogen, has been shown to induce apoptosis in vitro, no studies have been carried out to confirm and characterize its role in activating apoptosis in vivo. The present study investigated the effects of benzene inhalation on the epithelial cells lining the respiratory tract including bronchioles, terminal bronchioles, respiratory bronchioles and alveoli of male Sprague-Dawley rats. Inhalation of benzene 300 ppm for 7 days induced apoptotic changes in the parenchymal components in the lung that significantly exceeded the events of programmed cell death in normal control tissues. Apoptosis was confirmed by the electrophoretic analysis of internucleosomal DNA fragmentation of benzene-exposed lung tissues, which exhibited 180-200 bp laddering subunits indicative of genomic DNA degradation. Furthermore, semi-quantitative analysis of intracellular localization of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling TUNEL) showed a significant (p < 0.001) increase in the apoptotic index calculated for bronchiolar 73.5%, terminal bronchiolar (65%), and respiratory bronchiolar 60.8% segmental epithelial components as well as alveolar (55%) epithelia. Analysis of immunohistochemical expression of apoptosis-related gene products also supported the hypothesis that benzene can induce apoptosis in chemosensitive target cells in the lung parenchyma. Quantitative immunhistochemistry showed a statistically significant increase p < 0.001 in the immunoreactive staining index for cytochrome c, Apaf-1 (apoptosis activating factor-1), DNA fragmentation factor, and representative cysteine proteases including caspase-1, caspase-2L, caspase-8 and caspase-9. Thus this is the first study of the respiratory system that demonstrates that benzene inhalation induces lung cell apoptosis as confirmed by DNA electrophoresis, in situ nick end labeling, and the upregulation of apoptosis-related gene products that facilitate caspase

  15. Analysis of Apoptosis in Ultraviolet-Induced Sea Cucumber (Stichopus japonicus) Melting Using Terminal Deoxynucleotidyl-Transferase-Mediated dUTP Nick End-Labeling Assay and Cleaved Caspase-3 Immunohistochemistry.

    PubMed

    Yang, Jing-Feng; Gao, Rong-Chun; Wu, Hai-Tao; Li, Peng-Fei; Hu, Xian-Shu; Zhou, Da-Yong; Zhu, Bei-Wei; Su, Yi-Cheng

    2015-11-01

    The sea cucumber body wall melting phenomenon occurs under certain circumstances, and the mechanism of this phenomenon remains unclear. This study investigated the apoptosis in the ultraviolet (UV)-induced sea cucumber melting phenomenon. Fresh sea cucumbers (Stichopus japonicus) were exposed to UV radiation for half an hour at an intensity of 0.056 mW/cm(2) and then held at room temperature for melting development. The samples were histologically processed into formalin-fixed paraffin-embedded tissues. The apoptosis of samples was analyzed with the terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling (TUNEL) assay and cleaved caspase-3 immunohistochemistry. The emergence of TUNEL-positive cells speeds up between 0.5 and 2 h after UV irradiation. Cleaved caspase-3 positive cells were obviously detected in sample tissues immediately after the UV irradiation. These results demonstrated that sea cucumber melting induced by UV irradiation was triggered by the activation of caspase-3 followed by DNA fragmentation in sea cucumber tissue, which was attributed to apoptosis but was not a consequence of autolysis activity.

  16. Target-triggered three-way junction structure and polymerase/nicking enzyme synergetic isothermal quadratic DNA machine for highly specific, one-step, and rapid microRNA detection at attomolar level.

    PubMed

    Zhang, Qing; Chen, Feng; Xu, Feng; Zhao, Yongxi; Fan, Chunhai

    2014-08-19

    MicroRNAs (miRNAs) play important roles in many biological processes and are regarded as promising cancer biomarkers. Herein, a highly specific, one-step, and rapid miRNAs detection strategy with attomolar sensitivity has been developed on the basis of a target-triggered three-way junction (3-WJ) structure and polymerase/nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). To this end, 3-WJ probes (primer and template) are designed to selectively recognize target miRNA and form the stable 3-WJ structure to trigger ESQM, resulting in a high quadratic amplified signal. A high specificity is demonstrated by the excellent discrimination of even single-base mismatched homologous sequences with mismatched bases in varied locations (close to the 3'-end, the 5'-end, and the middle). In addition, a low detection limit down to 2 amol was achieved within 30 min. This sensitivity is much higher than those of most linear amplification-based approaches and is even comparable to those of some exponential amplification-based methods. Furthermore, the applicability of this method in complex samples was demonstrated by the analysis of cancer cell small RNA extracts, results of which were in good agreement with those obtained by a commercial miRNA kit and previously published data. The miRNA with a 3' end modification (2'-O-methylation), such as plant miRNA, was also successfully detected, confirming the good universality of the proposed strategy. It is worthwhile to point out that several well-established methods using miRNA as primer for polymerization reaction are of relatively poor performance in the analysis of these modified miRNA. Therefore, these merits endow the developed strategy with powerful implications for biological research and an effective diagnostic assay. PMID:25072308

  17. Computer Modeling of Hydrology, Weathering, and Isotopic Fractionation in Andrews Creek, Rocky Mountain National Park, Colorado for Water Years 1992 through 2012

    NASA Astrophysics Data System (ADS)

    Webb, R. M. T.; Parkhurst, D. L.; Mast, A.; Clow, D. W.

    2014-12-01

    The U.S. Geological Survey's (USGS) Water, Energy, and Biogeochemical Model (WEBMOD) was used to simulate hydrology, weathering, and isotopic fractionation in the 1.7 square kilometer Andrews Creek alpine watershed. WEBMOD includes hydrologic modules derived from the USGS Precipitation Runoff Modeling System, the National Weather Service Hydro-17 snow model, and TOPMODEL. PHREEQC, a geochemical reaction model, is coupled with the hydrologic model to simulate the geochemical evolution of waters as they evaporate, mix, and react within the landscape. Major solute concentrations and δ18O were modeled over the period 1992-2012. Variations of chloride and inorganic nitrogen respond almost entirely to variations in atmospheric deposition and preferential elution of snowpack. Both evaporation and melting result in isotopic enrichment of heavy isotopes in the residual snowpack throughout the summer. Magnesium and potassium, derived mostly from weathering with some atmospheric inputs, vary seasonally with uptake during the growing season and release during the fall and winter. The weathering of granitic minerals—oligoclase, biotite, chlorite, pyrite, calcite, and formation of secondary minerals—kaolinite, goethite, gibbsite, and smectite-illite—were selected as primary reactions based on mole-balance modeling of basin outflows. The rates of these reactions were quantified by calibrating WEBMOD to match observed concentrations and loads. Exported annual loads of most weathering products are highly correlated with discharge, whereas silica loads are less correlated with discharge, suggesting a source that is more active during dry years and less active during wet years. Potential sources include net dissolution of kaolinite and smectite-illite or mineralization of colloids with high silica content. WEBMOD is a valuable tool for simulating water quality variations in response to climate change, acid mine drainage, acid rain, biological transformations, and other

  18. Spatial distribution and compositional variation of APS minerals related to uranium deposits in the Kiggavik-Andrew Lake structural trend, Nunavut, Canada

    NASA Astrophysics Data System (ADS)

    Riegler, Thomas; Quirt, Dave; Beaufort, Daniel

    2016-02-01

    The Kiggavik-Andrew Lake structural trend consists of four mineralized zones, partially outcropping, lying 2 km south of the erosional contact with the unmetamorphosed sandstone and basal conglomerates of the Paleoproterozoic Thelon Formation. The mineralization is controlled by a major E-W fault system associated with illite and sudoite alteration halos developed in the Archean metagraywackes of the Woodburn Lake Group. Aluminum phosphate sulfate (APS) minerals from the alunite group crystallized in association with the clay minerals in the basement alteration halo as well as in the overlying sandstones, which underwent mostly diagenesis. APS minerals are Sr- and S-rich (svanbergite end-member) in the sedimentary cover overlying the unconformity, whereas they are light rare earth elements (LREE)-rich (florencite end-member) in the altered basement rocks below the unconformity. The geochemical signature of each group of APS minerals together with the petrography indicates three distinct generations of APS minerals related to the following: (1) paleoweathering of continental surfaces prior to the basin occurrence, (2) diagenetic processes during the burial history of the lower unit of the Thelon sandstones, and (3) hydrothermal alteration processes which accompanied the uranium deposition in the basement rocks and partially overlap the sedimentary-diagenetic mineral parageneses. In addition, the association of a first generation of APS minerals with both detrital cerium oxide and aluminum oxy-hydroxide highlights the fact that a part of the detrital material of the basal Thelon Formation originated from eroded paleolaterite (allochthonous regolith). The primary rare earth element (REE)-bearing minerals (e.g., monazite, REE carbonates, and allanite) of the host rocks were characterized to identify the potential sources of REE. The REE chemical composition highlights a local re-incorporation of the REE released from the alteration processes in the APS minerals of

  19. Segment-scale and intrasegment lithospheric thickness and melt variations near the Andrew Bain megatransform fault and Marion hot spot: Southwest Indian Ridge, 25.5°E-35°E

    NASA Astrophysics Data System (ADS)

    Takeuchi, Christopher S.; Sclater, John G.; Grindlay, Nancy R.; Madsen, John A.; Rommevaux-Jestin, CéLine

    2010-07-01

    We analyze bathymetric, gravimetric, and magnetic data collected on cruise KN145L16 between 25.5°E and 35°E on the ultraslow spreading Southwest Indian Ridge, where the 750 km long Andrew Bain transform domain separates two accretionary segments to the northeast from a single segment to the southwest. Similar along-axis asymmetries in seafloor texture, rift valley curvature, magnetic anomaly amplitude, magnetization intensity, and mantle Bouguer anomaly (MBA) amplitude within all three segments suggest that a single mechanism may produce variable intrasegment lithospheric thickness and melt delivery. However, closer analysis reveals that a single mechanism is unlikely. In the northeast, MBA lows, shallow axial depths, and large abyssal hills indicate that the Marion hot spot enhances the melt supply to the segments. We argue that along-axis asthenospheric flow from the hot spot, dammed by major transform faults, produces the inferred asymmetries in lithospheric thickness and melt delivery. In the southwest, strong rift valley curvature and nonvolcanic seafloor near the Andrew Bain transform fault indicate very thick subaxial lithosphere at the end of the single segment. We suggest that cold lithosphere adjacent to the eastern end of the ridge axis cools and thickens the subaxial lithosphere, suppresses melt production, and focuses melt to the west. This limits the amount of melt emplaced at shallow levels near the transform fault. Our analysis suggests that the Andrew Bain divides a high melt supply region to the northeast from an intermediate to low melt supply region to the southwest. Thus, this transform fault represents not only a major topographic feature but also a major melt supply boundary on the Southwest Indian Ridge.

  20. Spatial and Temporal Trends in the Carbon, Nitrogen, and Sulfur Isotopes of Stream DOM From 10 Watersheds at the HJ Andrews Experimental Forest.

    NASA Astrophysics Data System (ADS)

    Frentress, J.; Kendall, C.; Lajtha, K.; Jones, J.

    2008-12-01

    In order to better understand sources of dissolved organic matter (DOM) in streams from the small to large watershed scales, we initiated a one-year investigation of the chemical and isotopic characteristics of DOM at the HJ Andrews Experimental Forest (HJA) in Blue River, OR. DOM is a biologically significant loss from these watersheds, but its sources (forest floor, mineral soil, riparian zones, stream biota) are debated. Traditional chemical characterizations of DOM like SUVA and FI have been useful in conceptualizing and modeling streamflow sources, however, an improved method for assessing DOM quality is needed to adequately differentiate DOM from sources within the watershed. The isotopic characterization of inorganic molecules like nitrate has provided insight to the role of subsurface and surface processes governing the production and transport of critical nutrients, and yet to date, little work has been done to examine the usefulness of isotopic characterization of organically bound nutrients. We apply the isotopic characterization approach to DOM in order to better understand DOM production, transformation, and transport to streams in a range of watershed sizes. Major questions addressed in this research are: 1) Where in the watershed does stream DOM come from? 2) How do DOM sources vary temporally? 3) How do physical attributes of the watershed mediate DOM quality? A relatively new solid-phase extraction technique using C-18 resin was used to isolate DOM in water samples from 10 watersheds, ranging in size from 10 to 6200 hectares, on 3-week intervals from May 2007 to June 2008. The modified technique allowed for small (1 Liter) sample sizes and short processing times to reduce the costs of analysis. The capacity of carbon, nitrogen and sulfur isotopic characterizations of DOM, as well as traditional methods like SUVA and C:N, to predict physical watershed attributes (i.e. mean residence time, soil depth, elevation, gradient) and land use history (timber

  1. A comparison of mercury burdens between St. Marks National Wildlife Refuge and St. Andrew Bay, Florida: Evaluation of fish body burdens and physiological responses in largemouth bass, spotted seatrout, striped mullet, and sunfish

    USGS Publications Warehouse

    Huge, D.H.; Rauschenberger, R.H.; Wieser, F.M.; Hemming, J.M.

    2011-01-01

    Musculature from the dorsal region of 130 largemouth bass (Micropterus salmoides), 140 sunfish (Lepomis sp.), 41 spotted seatrout (Cynoscion nebulosus) and 67 striped mullet (Mugil cephalus) were collected from five estuarine and five freshwater sites within the St. Marks National Wildlife Refuge and two estuarine and two freshwater sites from St. Andrew Bay, Florida, United States of America. Musculature was analyzed for total mercury content, sagittal otoliths were removed for age determination and physiological responses were measured. Largemouth bass and sunfish from the refuge had higher mercury concentrations in musculature than those from the bay. Male spotted seatrout, male striped mullet, male and female sunfish and female largemouth bass had mercury burdens positively correlated with length. The majority of all four species of fish from both study areas contained mercury levels below 1.5 part per million, the limit for safe consumption recommended the Florida Department of Health. In comparison, a significant percentage of largemouth bass and sunfish from several sampled sites, most notably Otter Lake and Lake Renfroe within St. Marks National Wildlife Refuge, had mercury levels consistent with the health department's guidelines of 'limited consumption' or 'no consumption guidelines.'

  2. Sir Henry Solomon Wellcome and Dr Andrew Balfour: an enterprise on the Nile and the early foundation of public health and medical research in the Sudan (1899-1935).

    PubMed

    Elhadd, T A

    2015-01-01

    In Sudan, modern medical practice and medical research began soon after the creation of Anglo-Egyptian Sudan at the turn of the 20th century. The benevolent involvement of Sir Henry Solomon Wellcome, and the ingenious feat of his protégé Sir Andrew Balfour, was crucial to the strong foundation of that establishment. Sir Henry Wellcome provided the financial sponsorship plus influential, logistical and moral support. Dr Balfour put great energy into making the enterprise one of the most amazing medical achievements in colonial medicine. Improvement in the public health of the capital Khartoum was emulated by other doctors working in this vast country. Research was not restricted to tropical medicine; it also encompassed agricultural and chemical research. This helped with the establishment of the first modern medical school in the country in 1924 and resulted in the medical service in Sudan being described as one of the best in the world. Many British doctors flocked to Sudan to make a fortune and to set a path for their career back in Britain.

  3. Nick Sagan Reflects on Voyager 1 and the Golden Record

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2013-10-01

    When scientists confirmed on 12 September that NASA's Voyager 1 spacecraft had entered interstellar space (Eos, 94(39), 339, doi:10.1002/2013EO390003), the probe was acknowledged as the first human-made object to travel into that realm. The probe and its twin, Voyager 2, each carry a 12-inch gold-plated copper disk, known as the Golden Record.

  4. Millikan, Robert Andrews (1868-1953)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Physicist, born in Morrison, IL, Nobel prizewinner (1923) for his determination of the charge on the electron by Millikan's oil-drop experiment, and confirmation of Einstein's quantum photoelectric theory. Coined the term `cosmic rays'....

  5. The Future of the Andrew File System

    ScienceCinema

    None

    2016-07-12

    The talk will discuss the ten operational capabilities that have made AFS unique in the distributed file system space and how these capabilities are being expanded upon to meet the needs of the 21st century. Derrick Brashear and Jeffrey Altman will present a technical road map of new features and technical innovations that are under development by the OpenAFS community and Your File System, Inc. funded by a U.S. Department of Energy Small Business Innovative Research grant. The talk will end with a comparison of AFS to its modern days competitors.

  6. Wireless Andrew: Everywhere You Want To Be.

    ERIC Educational Resources Information Center

    Futhey, Tracy

    2000-01-01

    Describes the wireless local area network at Carnegie Mellon University. Highlights include classroom applications, particularly in the Business School; the use of laptop computers configured with wireless technology; handheld computers, including use for testing; and assuring appropriate uses of wireless technology. (LRW)

  7. Speaking Unassisted: Comments on a Paper by Andrews et al.

    ERIC Educational Resources Information Center

    Wingate, Marcel E.

    1983-01-01

    In a reply to a review of replicated findings on stuttering, the author adds 14 points concerning symptoms, prevalence, incidence, stutterer-nonstutterer differences, and variability of stuttering. He takes exception to the review's statements on treatment and theories of stuttering. (CL)

  8. Human reproductive cloning: an analysis of the Andrews Report.

    PubMed

    Little, Kim

    2002-01-01

    There is nothing like an overwhelming consensus of opinion to encourage a less than rigourous approach to analyzing complex ethical issues. Unfortunately, this is nowhere more apparent than in the discussion of human reproductive cloning contained in the federal House of Representatives Standing Committee on Legal and Constitutional Affairs' report on human cloning, released last August. The report may well fulfil the first half of its project, namely the empirical task of adequately summarizing and categorizing the various submissions made to the Committee. However, it is clearly inadequate as a discussion of the ethical and legal permissibility of human reproductive cloning.

  9. General Christopher C. Andrews: Leading the Minnesota Forestry Revolution

    ERIC Educational Resources Information Center

    Rice, Anna M.

    2002-01-01

    In the nineteenth century, America's burgeoning population certainly did grab all the timber it could. Vast pine forests stretched from Maine to Dakota, and the lumber industry voraciously consumed them from east to west. In 1800, the Minnesota territory was sparsely sprinkled with fur traders and American Indians. By 1850, its bounteous forests…

  10. Parents' struggles to rebuild family life after Hurricane Andrew.

    PubMed

    Coffman, S

    1996-01-01

    The purpose of this phenomenological study was to gain a deeper understanding of the meaning of parents' everyday experiences after a major natural disaster. In-depth interviews were conducted with 13 parents who lived in the hurricane-damaged area. The essence of being a parent emerged as "struggling to rebuild family life." The struggles were superimposed on top of ongoing issues such as divorce and job responsibilities. Parents described feelings of being thankful to be alive, being overwhelmed, being limited by environmental aftereffects, being responsible for children, balancing needs and roles, constantly changing amidst uncertainty, and finding meaning in the disaster. Study findings support the need for nursing interventions that address family needs, support strengths, and involve parents as active decision makers.

  11. "On the margins": A dialogue with Andrew Greeley.

    PubMed

    Moss, D M

    1990-12-01

    This dialogue is between two priests who share common interests from different perspectives. One is an Episcopalian and a psychoanalytic psychotherapist. The other is a Roman Catholic and a sociologist widely known for his controversial novels and political commentaries. Their conversation primarily focuses on the latter's ministry-especially his investigations into paranormal experiences and his use of fiction as a homiletical avenue. They also discuss: Christian atheism; the Resurrection as a metaphor; Real Presence and liturgical sensibility; contemporary ecumenical trends; celibacy and tenures of active ministry; sexual equality and population control; religious addiction; and examples of the mythic impact of cinema.

  12. A resolution honoring the life of Andrew Wyeth.

    THOMAS, 111th Congress

    Sen. Casey, Robert P., Jr. [D-PA

    2009-01-27

    01/27/2009 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S946-947; text as passed Senate: CR S947; text of measure as introduced: CR S899-900) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  13. New butane isomerization unit is unvieled by Andrews Petroleum

    SciTech Connect

    McWilliams, H.

    1990-06-01

    This article discusses the development of a butane isomerization unit which will help reduce butane surplus by fractionating it into other LPG products. Other features of this California project increase on-site storage.

  14. Processing of Nonconjugative Resistance Plasmids by Conjugation Nicking Enzyme of Staphylococci

    PubMed Central

    Pollet, Rebecca M.; Ingle, James D.; Hymes, Jeff P.; Eakes, Thomas C.; Eto, Karina Yui; Kwong, Stephen M.; Ramsay, Joshua P.; Firth, Neville

    2016-01-01

    ABSTRACT Antimicrobial resistance in Staphylococcus aureus presents an increasing threat to human health. This resistance is often encoded on mobile plasmids, such as pSK41; however, the mechanism of transfer of these plasmids is not well understood. In this study, we first examine key protein-DNA interactions formed by the relaxase enzyme, NES, which initiates and terminates the transfer of the multidrug resistance plasmid pSK41. Two loops on the NES protein, hairpin loops 1 and 2, form extensive contacts with the DNA hairpin formed at the oriT region of pSK41, and here we establish that these contacts are essential for proper DNA cleavage and religation by the full 665-residue NES protein in vitro. Second, pSK156 and pCA347 are nonconjugative Staphylococcus aureus plasmids that contain sequences similar to the oriT region of pSK41 but differ in the sequence predicted to form a DNA hairpin. We show that pSK41-encoded NES is able to bind, cleave, and religate the oriT sequences of these nonconjugative plasmids in vitro. Although pSK41 could mobilize a coresident plasmid harboring its cognate oriT, it was unable to mobilize plasmids containing the pSK156 and pCA347 variant oriT mimics, suggesting that an accessory protein like that previously shown to confer specificity in the pWBG749 system may also be involved in transmission of plasmids containing a pSK41-like oriT. These data indicate that the conjugative relaxase in trans mechanism recently described for the pWBG749 family of plasmids also applies to the pSK41 family of plasmids, further heightening the potential significance of this mechanism in the horizontal transfer of staphylococcal plasmids. IMPORTANCE Understanding the mechanism of antimicrobial resistance transfer in bacteria such as Staphylococcus aureus is an important step toward potentially slowing the spread of antimicrobial-resistant infections. This work establishes protein-DNA interactions essential for the transfer of the Staphylococcus aureus multiresistance plasmid pSK41 by its relaxase, NES. This enzyme also processed variant oriT-like sequences found on numerous plasmids previously considered nontransmissible, suggesting that in conjunction with an uncharacterized accessory protein, these plasmids may be transferred horizontally via a relaxase in trans mechanism. These findings have important implications for our understanding of staphylococcal resistance plasmid evolution. PMID:26728193

  15. Magnetic Contribution to Heat Capacity and Entropy of Nicke Ferrite (NiFe2O4)

    SciTech Connect

    S Ziemniak, L Anovitz, R Castelli

    2005-12-15

    The heat capacity of nickel ferrite was measured as a function of temperature over the range from 50 to 1200 C using a differential scanning calorimeter. A thermal anomaly was observed at 584.9 C, the expected Curie temperature, T{sub c}. The observed behavior was interpreted by recognizing the sum of three contributions: (1) lattice (vibrational), (2) a spin wave (magnetic) component and (3) a {lambda}-transition (antiferromagnetic-paramagnetic transition) at the Curie temperature. The first was modeled using vibrational frequencies derived from an experimentally-based ir absorption spectrum, while the second was modeled using a spin wave analysis that provided a T{sup 3/2} dependency in the low temperature limit, but incorporated an exchange interaction between cation spins in the octahedral and tetrahedral sites at elevated temperatures, as first suggested by Grimes [15]. The {lambda}-transition was fitted to an Inden-type model which consisted of two truncated power law series in dimensionless temperature (T/T{sub c}). Exponential equality was observed below and above T{sub c}, indicating symmetry about the Curie temperature. Application of the methodology to existing heat capacity data for other transition metal ferrites (AFe{sub 2}O{sub 4}, A = Fe, Co) revealed the same exponential equality, i.e., m = n = 5.

  16. Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding

    SciTech Connect

    Tewary, Sunil K.; Liang, Lingfei; Lin, Zihan; Lynn, Annie; Cotmore, Susan F.; Tattersall, Peter; Zhao, Haiyan; Tang, Liang

    2015-02-15

    Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45 Å resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins. - Highlights: • The structure of a parvovirus replication initiator protein has been determined; • The structure sheds light on mechanisms of ssDNA binding and cleavage; • The nickase active site is preconfigured for versatile metal ligand binding; • The binding site for the double-stranded replication origin DNA is identified; • A single domain integrates multiple functions in virus replication.

  17. Talking about Life

    NASA Astrophysics Data System (ADS)

    Impey, Chris

    2010-08-01

    Preface; Part I. Introduction Timothy Ferris, Iris Fry, Steven Dick, Ann Druyan, Pinky Nelson, Neil Tyson, Steve Benner and William Bains; Part II. Earth Roger Buick, Lynn Rothschild, John Baross, Joe Kirschvink, Andrew Knoll, Simon Conway Morris, Roger Hanlon and Lori Marino; Part III. Solar System Chris McKay, David Grinspoon, Jonathan Lunine, Carolyn Porco, Laurie Leshin, Guy Consolmagno and Peter Smith; Part IV. Exoplanets Alan Boss, Geoff Marcy, Debra Fischer, Sara Seager, David Charbonneau and Vikki Meadows; Part V. Frontiers Jill Tarter, Seth Shostak, Ray Kurzweil, Nick Bostrom, Paul Davies, Martin Rees, Ben Bova and Jennifer Michael Hecht; Reading list; Glossary; Index.

  18. Detecting Outliers in Marathon Data by Means of the Andrews Plot

    NASA Astrophysics Data System (ADS)

    Stehlík, Milan; Wald, Helmut; Bielik, Viktor; Petrovič, Juraj

    2011-09-01

    For an optimal race performance, it is important, that the runner keeps steady pace during most of the time of the competition. First time runners or athletes without many competitions often experience an "blow out" after a few kilometers of the race. This could happen, because of strong emotional experiences or low control of running intensity. Competition pace of half marathon of the middle level recreational athletes is approximately 10 sec quicker than their training pace. If an athlete runs the first third of race (7 km) at a pace that is 20 sec quicker than is his capacity (trainability), he would experience an "blow out" in the last third of the race. This would be reflected by reducing the running intensity and inability to keep steady pace in the last kilometers of the race and in the final time as well. In sports science, there are many diagnostic methods ([3], [2], [6]) that are used for prediction of optimal race pace tempo and final time. Otherwise there is lacking practical evidence of diagnostics methods and its use in the field (competition, race). One of the conditions that needs to be carried out is that athletes have not only similar final times, but it is important that they keep constant pace as much as possible during whole race. For this reason it is very important to find outliers. Our experimental group consisted of 20 recreational trained athletes (mean age 32,6 years±8,9). Before the race the athletes were instructed to run on the basis of their subjective feeling and previous experience. The data (running pace of each kilometer, average and maximal heart rate of each kilometer) were collected by GPS-enabled personal trainer Forerunner 305.

  19. Fascia Research Congress evidence from the 100 year perspective of Andrew Taylor Still.

    PubMed

    Findley, Thomas W; Shalwala, Mona

    2013-07-01

    More than 100 years ago A.T. Still MD founded osteopathic medicine, and specifically described fascia as a covering, with common origins of layers of the fascial system despite diverse names for individual parts. Fascia assists gliding and fluid flow and is highly innervated. Fascia is intimately involved with respiration and with nourishment of all cells of the body, including those of disease and cancer. This paper reviews information presented at the first three International Fascia Research Congresses in 2007, 2009 and 2012 from the perspective of Dr Still, that fascia is vital for organism's growth and support, and it is where disease is sown.

  20. Seizing Opportunities: Genie Tyburski--Ballard Spahr Andrews & Ingersoll, LLP, Philadelphia

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    Genie Tyburski did not set out to be a law librarian. When asked at Drexel's library school what kind of librarian she wanted to be, she was surprised that "a good one" was not one of the options. But six weeks into the semester, she landed a part-time cataloging job at Community Legal Services in Philadelphia; six months later she was the library…

  1. Multiple species of wild tree peonies gave rise to the 'king of flowers', Paeonia suffruticosa Andrews.

    PubMed

    Zhou, Shi-Liang; Zou, Xin-Hui; Zhou, Zhi-Qin; Liu, Jing; Xu, Chao; Yu, Jing; Wang, Qiang; Zhang, Da-Ming; Wang, Xiao-Quan; Ge, Song; Sang, Tao; Pan, Kai-Yu; Hong, De-Yuan

    2014-12-22

    The origin of cultivated tree peonies, known as the 'king of flowers' in China for more than 1000 years, has attracted considerable interest, but remained unsolved. Here, we conducted phylogenetic analyses of explicitly sampled traditional cultivars of tree peonies and all wild species from the shrubby section Moutan of the genus Paeonia based on sequences of 14 fast-evolved chloroplast regions and 25 presumably single-copy nuclear markers identified from RNA-seq data. The phylogeny of the wild species inferred from the nuclear markers was fully resolved and largely congruent with morphology and classification. The incongruence between the nuclear and chloroplast trees suggested that there had been gene flow between the wild species. The comparison of nuclear and chloroplast phylogenies including cultivars showed that the cultivated tree peonies originated from homoploid hybridization among five wild species. Since the origin, thousands of cultivated varieties have spread worldwide, whereas four parental species are currently endangered or on the verge of extinction. The documentation of extensive homoploid hybridization involved in tree peony domestication provides new insights into the mechanisms underlying the origins of garden ornamentals and the way of preserving natural genetic resources through domestication.

  2. 77 FR 59163 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    .... This change in condition would improve ecosystem ] health; increasing species diversity of native... CONTACT: Victor Wyant, 864-638-9568. Correction 1. In the Federal Register of March 8, 2010, in FR/Vol. 75... expected by December 2012. 2. In the Federal Register of March 8, 2010, in FR/Vol. 75, No. 44, on...

  3. Detectives Nemorin and Andrews Anti-Gun Trafficking Act of 2013

    THOMAS, 113th Congress

    Rep. King, Peter T. [R-NY-2

    2013-02-14

    04/08/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  4. Additional dates of Sir Andrew Smith's Illustrations of the Zoology of South Africa.

    PubMed

    Low, Martyn E Y; Evenhuis, Neal L

    2014-05-14

    We update the collation of the dates of publication of Smith's Illustrations of the Zoology of South Africa provided by Waterhouse (1880) and Barnard (1950, 1952). In the case of nine parts, we are able to provide more accurate dates of publication (including day-dates for seven of these parts). For workers of invertebrate taxonomy, we provide an accurate date of publication for W. S. Macleay's volume on Annulosa. 

  5. 76 FR 57043 - Andrew N. Finkel; Analysis of Proposed Consent Order to Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ... proposed order. This matter involves the advertising of a mobile software application (``app'') called Acne Pwner which respondent developed and sold in Google's Android Marketplace. Respondent claimed that Acne Pwner effectively treats acne. The instructions for this app directed consumers to hold the...

  6. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews.

    PubMed

    Chen, Yonggan; Gu, Fenglin; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-08-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. PMID:25979899

  7. A resolution electing Andrew B. Willison as the Sergeant at Arms and Doorkeeper of the Senate.

    THOMAS, 113th Congress

    Sen. Reid, Harry [D-NV

    2014-05-05

    05/05/2014 Submitted in the Senate, considered, and agreed to without amendment by Unanimous Consent. (consideration: CR S2625; text as passed Senate: CR S2649) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  8. Localization of beta-D-glucosidase activity and glucovanillin in vanilla bean (Vanilla planifolia Andrews).

    PubMed

    Odoux, E; Escoute, J; Verdeil, J-L; Brillouet, J-M

    2003-09-01

    The morphology, anatomy and histology of mature green vanilla beans were examined by light and transmission electron microscopy. Beans have a triangular cross-section with a central cavity containing seeds. Each angle is lined with tubular cells, or papillae, while the cavity sides consist of placental laminae. The epicarp and endocarp are formed by one or two layers of very small cells, while the mesocarp contains large, highly vacuolarized cells, the cytoplasm being restricted to a thin layer along the cell walls. The radial distributions of glucovanillin and beta-glucosidase activity, measured on p-nitrophenyl-beta-glucopyranoside and glucovanillin, are superimposable and show how beta-glucosidase activity increases from the epicarp towards the placental zone, whereas glucovanillin is exclusively located in the placentae and papillae. Subcellular localization of beta-glucosidase activity was achieved by incubating sections of vanilla beans in a buffer containing 5-bromo-4-chloro-3-indolyl-beta-d-glucopyranoside as a substrate. Activity was observed in the cytoplasm (and/or the periplasm) of mesocarp and endocarp cells, with a more diffuse pattern observed in the papillae. A possible mechanism for the hydrolysis of glucovanillin and release of the aromatic aglycon vanillin involves the decompartmentation of cytoplasmic (and/or periplasmic) beta-glucosidase and vacuolar glucovanillin.

  9. Purification and characterization of vanilla bean (Vanilla planifolia Andrews) beta-D-glucosidase.

    PubMed

    Odoux, Eric; Chauwin, Audrey; Brillouet, Jean-Marc

    2003-05-01

    Vanilla bean beta-D-glucosidase was purified to apparent homogeneity by successive anion exchange, hydrophobic interaction, and size-exclusion chromatography. The enzyme is a tetramer (201 kDa) made up of four identical subunits (50 kDa). The optimum pH was 6.5, and the optimum temperature was 40 degrees C at pH 7.0. K(m) values for p-nitrophenyl-beta-D-glucopyranoside and glucovanillin were 1.1 and 20.0 mM, respectively; V(max) values were 4.5 and 5.0 microkat.mg(-1). The beta-D-glucosidase was competitively inhibited by glucono-delta-lactone and 1-deoxynojirimycin, with respective K(i) values of 670 and 152 microM, and not inhibited by 2 M glucose. The beta-D-glucosidase was not inhibited by N-ethylmaleimide and DTNB and fully inhibited by 1.5-2 M 2-mercaptoethanol and 1,4-dithiothreitol. The enzyme showed decreasing activity on p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-galactopyranoside, and p-nitrophenyl-beta-D-xylopyranoside. The enzyme was also active on prunasin, esculin, and salicin and inactive on cellobiose, gentiobiose, amygdalin, phloridzin, indoxyl-beta-D-glucopyranoside, and quercetin-3-beta-D-glucopyranoside.

  10. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews.

    PubMed

    Chen, Yonggan; Gu, Fenglin; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-08-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation.

  11. Planning for Andrew: The Use of COACH and VISTA in an Inclusive Preschool Program.

    ERIC Educational Resources Information Center

    Giangreco, Michael F.; Whiteford, Timothy; Whiteford, Lucie; Doyle, Mary Beth

    1998-01-01

    This case study chronicles the use of two educational planning tools, COACH (Choosing Outcomes and Accommodations for Children) and VISTA (Vermont Interdependent Services Team Approach) for a 4-year-old with Down syndrome who attends a general education preschool. The decision-making process and follow-up interviews are described, illustrating the…

  12. A resolution honoring former Senator and Rear Admiral Jeremiah Andrew Denton, Jr.

    THOMAS, 113th Congress

    Sen. Sessions, Jeff [R-AL

    2014-03-31

    03/31/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S1881; text as passed Senate: CR S1874) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  13. Utilization of Special Forces medical assets during disaster relief: the Hurricane Andrew experience.

    PubMed

    Godbee, D C; Odom, J W

    1997-02-01

    Special Forces units and their innate assets are presented as the ideal first-response unit to natural disasters due to their breadth of skill, speed of response, and ability to work independently in remote areas. "Green Beret" soldiers are particularly suited to work under the most extreme hardships, with little or no supervision, and can demonstrate tremendous amounts of initiative and creativity in unique and changing situations. The compact, versatile, and adaptable detachments of which Special Forces Groups are composed can serve as vital resources in humanitarian and disaster relief operations as well as in combat.

  14. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews

    PubMed Central

    Chen, Yonggan; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-01-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. PMID:25979899

  15. Nickel(I) and nickel(III) complexes of substituted tetraaza macrocycles formed by pulse radiolysis and electrochemistry of nickel(II) precursors

    SciTech Connect

    Bernhardt, P.V.; Lawrance, G.A.; Sangster, D.F.

    1988-11-02

    The square-planar nickel(II) complexes of the ligands 8-methyl-8-nitro-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 8-amino-8-methyl-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 3,7-bis(2-aminoethyl)-1,3,5,7-tetraazabicyclo(3.3.1)nonane, and 9-methyl-9-nitro-1,4,7,11-tetraazacyclotridecane (I-IV) react rapidly with hydroxyl radicals and aquated electrons (e/sub aq/). The initial transient products of these reactions decay via first-order kinetics within a few milliseconds in neutral aqueous solution at 22/degrees/C in all cases. Electronic spectra and decay rate constants, as well as formation rate constants, are reported for all transients. Reaction of the nitro-substituted complexes with e/sub aq/ led to electron addition to the nitro group rather than to the metal center; otherwise, a Ni/sup I/ transient is observed. Following reaction with OH, the product of the initial decay remains a Ni/sup III/ species. This is more long-lived, and stabilization of Ni/sup III/ by axial coordination of the pendant amine in II is indicated. No notable stabilization of Ni/sup I/ or Ni/sup III/ from the presence of the bicyclic azamethylene football in I-III occurs. Cyclic voltammetry in acetonitrile identified both one-electron oxidation and one-electron reduction processes for the nickel(II) complexes, as well as nitro group reduction, where this group was pendant to the macrocycle. 34 references, 3 figures, 3 tables.

  16. Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification

    PubMed Central

    Wei, Hua; Zhao, Guojie; Hu, Tianyu; Tang, Suming; Jiang, Jiquan; Hu, Bo; Guan, Yifu

    2016-01-01

    We used a novel asymmetric cleavage analysis method based on rolling circle amplification (RCA) to determine the effects of LNA modification of substrate on the two subunits of R.BbvCI cleavage. We designed two sets of cleavage circular substrates by using two different ligation strategies and analyzed the single strand cleavage efficiency affected by different modification positions both from the cleaved strands and the uncleaved strands. Results showed that the effects of LNA on cleavage rates of modified strands and unmodified strands were both site-dependent. The Nb.BbvCI and Nt.BbvCI were affected by LNA modification in different way. Most of the modification positions showed strong inhibition of both of these two nickases cleavage. However, the modification in T3 position of bottom strand hardly affected both of the two nickases activities. The results suggested an intimated interaction between the two subunits of R.BbvCI, and the T3 position in bottom strand might be a less tight position which was hard to be disturbed. PMID:27582033

  17. Relationship of Ordovician and Silurian reservoir development to unconformities at Midland farms and Inez fields, Andrews County, Texas

    SciTech Connect

    Mear, C.E.; Becher, J.W.

    1986-03-01

    Hydrocarbons are being produced at Midland Farms and Inez fields from Ellenburger dolomites and Fusselman limestones. Reservoirs developed there during Ordovician and Silurian periods of minor folding and faulting, followed by regional uplift and subaerial exposure of the carbonates. Vuggy, cavernous, and solution-enlarged fracture porosity was developed in the Lower Ordovician Ellenburger dolomites prior to deposition of the overlying Middle Ordovician shales of the Simpson Group. Vuggy and cavernous porosity developed in the Lower Silurian Fusselman crinoid-ostracod-pellet packstones and grainstones before deposition of the overlying Silurian Wristen shales. Montoya siliceous limestones of Late Ordovician age were truncated during a period of pre-Silurian erosion, but porosity development is not indicated in Montoya rock cuttings. Only minor amounts of porosity developed in the Lower to Middle Devonian Thirty-one packstones and wackestones as a result of uplift and erosion in the Middle Devonian. Regional compression during the post-Mississippian enhanced doubly plunging anticlines now having up to 91 m (300 ft) of closure at the Ellenburger through Thirty-one formations at Midland Farms and Inez fields. Fractures may have developed in Paleozoic limestones during this period of folding, but reservoir enhancement appears to have resulted only in the Ellenburger dolomites. Representative porosity measurements of the Ellenburger and Fusselman pay zones cannot be made from wireline log calculations, due to the fractured, vuggy, and cavernous nature of the porosity.

  18. Understanding and Helping Families: A Cognitive-Behavioral Approach, by Andrew I. Schwebel and Mark A. Fine.

    ERIC Educational Resources Information Center

    Wilson, Stephan M.

    1994-01-01

    Reviews and evaluates 1993 text integrating theory, research, and application. Describes book as useful for researchers and practitioners as well as for instructors who emphasize how family research and theory inform family practice. Outlines text by three sections: model description, creation and maintenance of healthy families, and critique of…

  19. 77 FR 15600 - Special Local Regulation; Emerald Coast Super Boat Grand Prix; Saint Andrew Bay; Panama City, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-16

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; Emerald Coast Super Boat Grand... crews, vessels, and persons on navigable waters during the Emerald Coast Super Boat Grand Prix high... received an application for a Marine Event Permit on January 31, 2011 from Super Boat International,...

  20. Multiple species of wild tree peonies gave rise to the ‘king of flowers’, Paeonia suffruticosa Andrews

    PubMed Central

    Zhou, Shi-Liang; Zou, Xin-Hui; Zhou, Zhi-Qin; Liu, Jing; Xu, Chao; Yu, Jing; Wang, Qiang; Zhang, Da-Ming; Wang, Xiao-Quan; Ge, Song; Sang, Tao; Pan, Kai-Yu; Hong, De-Yuan

    2014-01-01

    The origin of cultivated tree peonies, known as the ‘king of flowers' in China for more than 1000 years, has attracted considerable interest, but remained unsolved. Here, we conducted phylogenetic analyses of explicitly sampled traditional cultivars of tree peonies and all wild species from the shrubby section Moutan of the genus Paeonia based on sequences of 14 fast-evolved chloroplast regions and 25 presumably single-copy nuclear markers identified from RNA-seq data. The phylogeny of the wild species inferred from the nuclear markers was fully resolved and largely congruent with morphology and classification. The incongruence between the nuclear and chloroplast trees suggested that there had been gene flow between the wild species. The comparison of nuclear and chloroplast phylogenies including cultivars showed that the cultivated tree peonies originated from homoploid hybridization among five wild species. Since the origin, thousands of cultivated varieties have spread worldwide, whereas four parental species are currently endangered or on the verge of extinction. The documentation of extensive homoploid hybridization involved in tree peony domestication provides new insights into the mechanisms underlying the origins of garden ornamentals and the way of preserving natural genetic resources through domestication. PMID:25377453

  1. Introduction: Andrew Thomson and the Centre for Metalloprotein Spectroscopy and Biology at the University of East Anglia.

    PubMed

    Wilson, Michael T

    2008-12-01

    The present article briefly relates the early history and growth of the Centre for Metalloprotein Spectroscopy and Biology at UEA (University of East Anglia) under the joint directorship of A.J. Thomson and C. Greenwood, and charts the exceptional success that this centre has had in fostering bioinorganic chemistry in the U.K. and the impact that it has had internationally.

  2. 76 FR 12094 - Andrew Peklo III; Notice of Application Tendered for Filing With the Commission and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ....net . i. FERC Contact: Steve Kartalia, (202) 502-6131 or Stephen.kartalia@ferc.gov . j. Cooperating....gov/docs-filing/ferconline.asp). Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You...

  3. 78 FR 27126 - East Bay, St. Andrews Bay and the Gulf of Mexico at Tyndall Air Force Base, Florida; Restricted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... an anonymous access system, which means we will not know your identity or contact information unless... and crafts (sailing, motorized, and/or rowed/towed or otherwise self-propelled), private and... craft, including pleasure vessels and crafts (sailing, motorized, and/or rowed/towed or otherwise...

  4. Comparison of four kinds of extraction techniques and kinetics of microwave-assisted extraction of vanillin from Vanilla planifolia Andrews.

    PubMed

    Dong, Zhizhe; Gu, Fenglin; Xu, Fei; Wang, Qinghuang

    2014-04-15

    Vanillin yield, microscopic structure, antioxidant activity and overall odour of vanilla extracts obtained by different treatments were investigated. MAE showed the strongest extraction power, shortest time and highest antioxidant activity. Maceration gave higher vanillin yields than UAE and PAE, similar antioxidant activity with UAE, but longer times than UAE and PAE. Overall odour intensity of different vanilla extracts obtained by UAE, PAE and MAE were similar, while higher than maceration extracts. Then, powered vanilla bean with a sample/solvent ratio of 4 g/100 mL was selected as the optimum condition for MAE. Next, compared with other three equations, two-site kinetic equation with lowest RMSD and highest R²(adj) was shown to be more suitable in describing the kinetics of vanillin extraction. By fitting the parameters C(eq), k₁, k₂, and f, a kinetics model was constructed to describe vanillin extraction in terms of irradiation power, ethanol concentration, and extraction time.

  5. "Show Me Where You Study!"--An Interactive Project between German Language Students in Nottingham and St Andrews

    ERIC Educational Resources Information Center

    Hartung, Insa; Reisenleutner, Sandra

    2016-01-01

    Interactive projects among students of a Common European Framework of Reference for languages (CEFR) A1+/A2 level seem difficult to set up due to the limited language repertoire of the students. Thus, our aim was to take up the challenge and start a project with the objective of applying their language skills. We chose a collaborative approach to…

  6. Assessing the skill of hydrology models at simulating the water cycle in the HJ Andrews LTER: Assumptions, strengths and weaknesses

    EPA Science Inventory

    Simulated impacts of climate on hydrology can vary greatly as a function of the scale of the input data, model assumptions, and model structure. Four models are commonly used to simulate streamflow in model assumptions, and model structure. Four models are commonly used to simu...

  7. 70 FR 22719 - In the Matter of Andrew Siemaszko; Order Prohibiting Involvement in NRC-Licensed Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2005-05-02

    ... discovered on top of the thermal insulation under the CRD flanges. Boron accumulated on the top of the thermal insulation resulted from the CRD leakage. The CRD leakage issues are discussed in CR 2000-0782... the reactor head and the thermal insulation was removed during the cleaning process performed under...

  8. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... by latitude 30°09′45.6″ N., longitude 85°44′20.6″ W.; thence proceed 100 feet waterward of the mean high water line directly to a point at latitude 30°09′46.8″ N., longitude 85°44′20.6″ W. From this... mean high water line to a point at latitude 30°10′16.7″ N., longitude 85°45′01.2″ W. located east...

  9. In response to "The Knowledge of Our Knowledge": a reflection on McAndrews' view of epistemology.

    PubMed

    Winterstein, James

    2012-12-01

    This commentary considers one of the articles published in the first volume of this journal and reflects on the status of research and knowledge at that time. The chiropractic profession has witnessed advancement in the use of the scientific method in the past several decades, and scholarly journals have helped support this substantial growth.

  10. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... stopping through this area at any time unless warned by helicopter or patrol boat. (4) The area will be patrolled by helicopter/vessels during periods of hazardous military activity. Verbal warnings...

  11. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... stopping through this area at any time unless warned by helicopter or patrol boat. (4) The area will be patrolled by helicopter/vessels during periods of hazardous military activity. Verbal warnings...

  12. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... stopping through this area at any time unless warned by helicopter or patrol boat. (4) The area will be patrolled by helicopter/vessels during periods of hazardous military activity. Verbal warnings...

  13. 78 FR 76812 - In the Matter of: Andrew Vincent O'Donnell, Inmate Number-62355-019, USP Atlanta, U.S...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE... (78 FR 49107 (August 12, 2013)), has continued the Regulations in effect under the International..., or is intended to be, exported from the United States; or E. Engage in any transaction to service...

  14. Depositional and diagenetic controls on porosity permeability and oil production in McFarland/Magutex (Queen) reservoirs, Andrews County, west Texas

    SciTech Connect

    Holtz, M.H. )

    1991-03-01

    The McFarland/Magutex Queen reservoir complex lies along the northeastern edge of the Central basin platform in the west Texas Permian basin and produces oil from the Permian Queen Formation. Current production from this complex totals 42 million stock-tank barrels (MMSTB) of an estimated 219 MMSTB of original oil in place, with an estimated 90 MMSTB of remaining mobile oil (RMO). The gross pay interval contains two parasequences consisting of progradational, 30-ft-thick, upward-shoaling facies packages. Facies include shoreface, mixed tidal channel and intertidal flat, and supratidal. Elongate shoreface facies are characterized by poorly consolidated, massive to thinly laminated sandstones. The supratidal facies, which act as permeability barriers, are characterized by algal-laminated dolostone and nodular, laminated, and massive anhydrite containing halite and gypsum pseudomorphs. Highest production and the largest amount of the 90 MMSTB of RMO is associated with the shoreface and tidal-channel facies. Bulk pore volume storage capacity and permeability are also highest within these two facies. Sandstones are arkosic, containing anhydrite and dolomite cements. Accessory minerals are clays, authigenic feldspar, and dolomite. Three main pore types are recognized: interparticle, moldic and intraconstituent, and micropores. Moldic and intraconstituent porosity is associated with leached feldspars and anhydrite cement dissolution. Microporosity is associated with syndepositional, grain-coating corrensite, dissolution-enhanced feldspar cleavage planes, and authigenic multifaceted dolomite. Microporosity derived from clays and dolomite is formed preferentially in tidal-channel and intertidal flat facies.

  15. NIOSH testimony on Kuwait before the subcommittee on hospitals and health care, committee on veterans' affairs by J. S. Andrews, September 16, 1992

    SciTech Connect

    Not Available

    1992-09-16

    The testimony summarizes potential adverse health effects related to service in the Persian Gulf as presented by the Department of Health and Human Services. An estimated 9,000 workers from 43 different countries battled the burning oil wells in Kuwait from February 1991 through early November 1991 when the last was capped. Exposures and health effects in US military personnel, Kuwaiti citizens, and fire fighters were described. The hazards to the soldiers were largely dependent on the concentration of the pollutants in the air near the camps. Fortunately, the plume from the fires rose up to 10,000 and 12,000 feet, mixed with the air and then dispersed for several thousand miles downwind over a period of several weeks. The particles and gases contained in the plume were diluted as the plume travelled. Even so, some minor respiratory problems were present among the soldiers. Some of the hydrocarbons measured at low concentrations have been shown to produce cancer in laboratory animals only when present at higher levels of exposure. Based on the exposure information gathered, the author concludes that there will not likely be a detectable increase in lung cancer in Gulf War Veterans as a result of the oil well fires.

  16. Caring: some reflections on the impact of the culture care theory by McFarland & Andrews and a conversation with Leininger.

    PubMed

    Clarke, Pamela N; McFarland, Marilyn R; Andrews, Margaret M; Leininger, Madeleine

    2009-07-01

    This column is the first of two with a special focus on the construct of caring. In this dialogue, two Leininger scholars together address the questions related to the global impact on practice and the contribution of the model to scientific development in nursing. Then, in a special conversation, nurse theorist Madeleine Leininger offers her view of the impact of her work as well as some of her early experiences.

  17. Society News: RAS Awards 2012; Prof. Andy Fabian; Prof. John C Brown; Prof. Andrew Fazakerley; Dr. Mike Irwin; Joss Bland-Hawthorn

    NASA Astrophysics Data System (ADS)

    2012-02-01

    Each year the RAS recognizes outstanding achievement in astronomy and geophysics by the award of medals and prizes. Candidates are nominated by Fellows and the awards made by a committee of Fellows, ensuring that these scientists have earned the respect and admiration of their peers in the research community.

  18. From School to Adult Living: A Forum on Issues and Trends. An Interview with Lou Brown, Andrew S. Halpern, Susan Brody Hasazi, and Paul Wehman.

    ERIC Educational Resources Information Center

    Clark, Gary M., Ed.; Knowlton, H. Earl, Ed.

    1987-01-01

    An interview with four leaders (L. Brown, A. Halpern, S. Hasazi, and P. Wehman) on the conceptualization and implementation of transition programming for handicapped students focuses on such issues as the role of political factors, the possibility of effective school/adult service agency coordination, the importance of social skills, and emerging…

  19. Chemometric analysis of chromatographic fingerprints shows potential of Cyclopia maculata (Andrews) Kies for production of standardized extracts with high xanthone content.

    PubMed

    Schulze, Alexandra E; de Beer, Dalene; de Villiers, André; Manley, Marena; Joubert, Elizabeth

    2014-10-29

    Cyclopia species are used for the production of honeybush tea and food ingredient extracts associated with many health benefits. A species-specific high-performance liquid chromatography (HPLC) method for Cyclopia maculata, developed and validated, allowed quantification of the major compounds in extracts from "unfermented" and fermented C. maculata. Two xanthones were tentatively identified for the first time in a Cyclopia species, whereas an additional four compounds were tentatively identified for the first time in C. maculata. "Fermentation" (oxidation) decreased the content of all compounds, with the exception of vicenin-2. Similarity analysis of the chromatographic fingerprints of unfermented C. maculata aqueous extracts showed extremely low variation (r ≥ 0.97) between samples. Some differences between wild-harvested and cultivated seedling plants were, however, demonstrated using principal component analysis. Quantitative data of selected compounds confirmed the low level of variation, making this Cyclopia species ideal for the production of standardized food ingredient extracts.

  20. One World, Many Cultures. Papers from the International Conference on Adult Education and the Arts (4th, St. Andrews, Scotland, July 10-14, 1995).

    ERIC Educational Resources Information Center

    Jones, David, Ed.; McConnell, Bridget, Ed.; Normie, Gerald, Ed.

    The following papers are included: "Foreword" (Bridget McConnell); "Introduction" (David J. Jones); opening addresses by George Robertson MP, Shadow Scottish Secretary, and by Charlie McConnell, Executive Director, Scottish Community Education Council; and speech by Christine Hamilton, Deputy Director, Scottish Arts Council; "Keynote Speech:…

  1. 77 FR 59001 - Fee for Services To Support FEMA's Offsite Radiological Emergency Preparedness Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-25

    ... INFORMATION CONTACT: Andrew Mitchell, Director, Technological Hazards Division, Department of Homeland...), or (email) Andrew.Mitchell2@fema.dhs.gov . SUPPLEMENTARY INFORMATION: As authorized by 42...

  2. Creating a multi-gas proxy for Delta 14C and atmospheric fossil fuel-CO2. Kevin Coakley, John Miller , Scott Lehman, Stephen Montzka, Colm Sweeney, Arlyn Andrews , Ben Miller

    NASA Astrophysics Data System (ADS)

    Coakley, K. J.; Miller, J. B.; Lehman, S.; Montzka, S. A.; Andrews, A. E.; Miller, B. R.

    2013-12-01

    The C14:C12 ratio of atmospheric CO2 (expressed as Delta 14C) is the gold standard measurement to derive the portion of observed atmospheric CO2 gradients resulting from combustion of fossil fuels (CO2-ff). This is because fossil fuels are devoid of 14C, unlike all other sources and sinks that impact atmospheric Delta14C. With enough 14C measurements, independent, 'top-down', estimation of US fossil fuel-CO2 emissions should be possible. However, our ability to make carbon-14 measurements is severely constrained by cost, accessibility to accelerator mass spectrometers (AMS) and the volume of air required to make high precision (~0.2 %) measurements of 14CO2 (mixing ratio is ~ 4e-16 mol/mol). Thus, Delta 14C is currently measured in only a small subset of NOAA/ESRL Global Monitoring Division (GMD) tall-tower and aircraft air samples. Here, we present a Projection Pursuit Regression (PPR) model to predict CO2-ff measured at different times and altitudes in terms of surrogate gases that are more widespread and relatively inexpensive to measure. This method would, in effect, allow expansion of Delta 14C measurements by factor of ~3 or 4 throughout North America. To create a proxy for CO2-ff, we take advantage of the observed correlations between (Delta 14C-derrived) CO2-ff and regional-scale enhancements of a wide array of anthropogenic gases, like CO, SF6, and halo- and hydro-carbons. We select the complexity and form of the PPR model by cross validation where validation data prediction error is minimized. In cross validation, the prediction model is based on the training data and not the validation data. We quantify prediction model performance with test data excluded from the model development process. According to cross validation, the PPR model is superior to a simpler linear model. Comparison with test CO2-ff data shows that CO2-ff can be predicted with a root mean square error of 1.1 ppm, only slightly higher than the Delta 14C-precision limit for CO2-ff of 1 ppm. Applying the PPR model to the large NOAA/ESRL/GMD database of surrogate gases, we can create a large set of synthetic CO2-ff data for the US. Finally, we will discuss the application of this large data set of lower-precision CO2-ff to top down determination of US fossil fuel CO2 emissions by presenting results from an Observation System Simulation Experiment (OSSE).

  3. Installation-Restoration Program. Phase 2. Confirmation/quantification. Stage 1. Problem confirmation study: Otis Air National Guard Base, Massachusetts, Air National Guard Support Center, Andrews Air Force Base, Maryland. Final technical report, November 1983-July 1985

    SciTech Connect

    Kraybill, R.L.; Smart, G.R.; Bopp, F.

    1985-09-04

    A Problem Confirmation Study was performed at seven sites on Otis Air National Guard Base: the Current and Former Training Areas, the Base Landfill, the Nondestructive Inspection Laboratory, the Fuel Test Dump Site, the Railyard Fuel Pumping Station, and the Petrol Fuel Storage Area. The field investigation was conducted in two stages, in November 1983 through January 1984, and in October through December 1984. Resampling was performed at selected locations in April and July 1985. A total of 11 monitor wells were installed and sampled and test-pit investigations were conducted at six sites. In addition, the contents of a sump tank, and two header pipes for fuel-transmission lines were sampled. Analytes included TOC, TOX, cyanide, phenols, Safe Drinking Water metals, pesticides and herbicides, and in the second round, priority-pollutant volatile organic compounds and a GC fingerprint scan for fuel products. On the basis of the field-work findings, it is concluded that, to date, water-quality impacts on ground water from past activities have been minimal.

  4. The Role of Science in Managed Aquifer Recharge--the Equus Beds aquifer near Wichita, Kansas Andrew Ziegler, Director Brian Kelly, Office Chief Michael Jacobs, Manager of Water Planning and Production Debra Ary, Engineer, Water Systems Planning (Invited)

    NASA Astrophysics Data System (ADS)

    Ziegler, A. C.; Jacobs, M.; Ary, D.; Kelly, B.

    2013-12-01

    Data collection and interpretation using statistical, geochemical, and numerical simulation tools are essential parts of a long-term cooperative study between the city of Wichita, U.S. Geological Survey, and others to describe water quantity and quality conditions in a 165 square-mile part of the Equus Beds aquifer and Arkansas and Little Arkansas Rivers. The Equus Beds aquifer, eastern part of the High Plains Aquifer in south-central Kansas, is a vital water resource for agriculture and city of Wichita. Withdrawals for public supply began in the 1940s and agricultural irrigation began in the 1950-60s. These withdrawals led to water-level declines of up to 40 feet (historic low in 1993), a storage loss of 250,000 acre feet compared to predevelopment, and may enhance movement of chloride contamination from a past oilfield disposal area near Burrton and from natural chloride along the Arkansas River. Monitoring data and modeling show chloride near Burrton moved about 3 miles in 45 years, is about 1 mile away from the nearest public supply wells, and will continue to move for decades to centuries making the water unusable for irrigation or water supply without treatment. These concerns led to development of Wichita's 1993 integrated local water-supply plan that increased use of Cheney Reservoir and implemented aquifer storage and recovery (ASR) within the aquifer using high flows from the Little Arkansas River. ASR benefits include replacing depleted storage and slowing chloride movement. Decreased withdrawals, increased precipitation, and artificial recharge increased water levels and added 100,000 acre feet of storage through 2010, but drought since 2011 has increased withdrawals. A calibrated model will be used to simulate transport of chloride under several withdrawal scenarios using MODFLOW coupled with SEAWAT. Since 1995, water-quality data collection for more than 400 organic and inorganic compounds in surface water, treated source water for artificial recharge, and groundwater identified indicator bacteria, atrazine, chloride, sodium, nitrate, arsenic, iron, and manganese as constituents of concern exceeding water-quality criteria in baseline samples. Techniques were developed to estimate Little Arkansas River water quality in real-time for treatment. Geochemical modeling using PHREEQC and PHAST shows that groundwater quality is not changed if groundwater and recharge water are of similar redox potential. If different, calcite or metal hydroxides may precipitate and decrease water infiltration. A network of 38 locations with shallow and deep wells characterizes the recharge quantities and qualities for the city of Wichita to withdraw when needed from storage. Through 2013, the Demonstration project and Phase 1 and 2 facilities (capacity 40 MGD) have artificially recharged about 2 billion gallons. Total construction costs are about $300,000,000. Data-collection, interpretative geochemical and numerical simulations and water-quality transport modeling tools developed in the past 70 years are a scientific foundation to effectively and objectively manage this aquifer system.

  5. Letter to the Editor Re: Andrew Turner, Emily R. Kearl, Kevin R. Solman Lead and other toxic metals in playground paints from South West England Science of the Total Environment 544 (2016) 460-466.

    PubMed

    Gottesfeld, Perry; Jacobs, David E

    2016-08-15

    Turner's paper emphasizes "oral bioaccessibility" instead of focusing solely on total lead content. There is no evidence that solubility testing for lead levels in paint correlates with absorption or blood lead levels in exposed children. There are many considerations in determining exposure hazards to paint that are not evaluated in assessing solubility. Although we strongly support the conclusions and recommendations of the study, we are concerned that by reporting "oral bioaccessibility" others will focus on solubility in developing regulatory standards for lead levels in paint or in conducting exposure assessments. Standards for lead in paint should continue to be based on total lead content, not "oral bioaccessibility." PMID:27260620

  6. Useful Method for the Preparation of Low-Coordinate Nickel(I) Complexes via Transformations of the Ni(I) Bis(amido) Complex K{Ni[N(SiMe3)(2,6- (i) Pr2-C6H3)]2}

    PubMed

    Lipschutz, Michael I; Tilley, T Don

    2014-10-13

    A convenient method of preparing two- and three-coordinate Ni(I) complexes of the form L-Ni(I)-X (L = P (t) Bu3, P (i) Pr3, DPPE, NHC; X = -N(SiMe3)(2,6- (i) Pr-C6H3), -O(2,6- (t) Bu2-4-Me-C6H2)) is reported. Protonation of the easily prepared anionic Ni(I) bis(amido) complex K{Ni[N(SiMe3)(2,6- (i) Pr-C6H3)]2} in the presence of an appropriate L-type ligand results in loss of HN(SiMe3)(2,6- (i) Pr-C6H3) and trapping of the resulting neutral Ni(I)-amido fragment to yield neutral, paramagnetic, two- and three-coordinate Ni(I) complexes. Protonation of these neutral amido complexes by the bulky phenol HO(2,6- (t) Bu2-4-Me-C6H2) results in loss of the second amido moiety and trapping by the resulting phenoxide to yield Ni(I)-O(2,6- (t) Bu2-4-Me-C6H2) complexes. The hapticity of the phenoxide ligand is influenced by the π-accepting ability of the L-type ligand. Where L = P (t) Bu3, a poor π-acceptor, the phenoxide acts as a π-acceptor and adopts a η(5)-bonding mode through dearomatization of the phenyl ring. When L = NHC, a competent π-acceptor, the phenoxide acts as a π-donor, adopting a η(1)-bonding mode through the O atom. The modular nature of this synthetic strategy allows variation of both the L- and X-type ligands of the complex in a stepwise fashion and should be extendable to a wide variety of ligand types for new Ni(I) complexes.

  7. Useful Method for the Preparation of Low-Coordinate Nickel(I) Complexes via Transformations of the Ni(I) Bis(amido) Complex K{Ni[N(SiMe3)(2,6-iPr2-C6H3)]2}

    PubMed Central

    2015-01-01

    A convenient method of preparing two- and three-coordinate Ni(I) complexes of the form L–NiI–X (L = PtBu3, PiPr3, DPPE, NHC; X = −N(SiMe3)(2,6-iPr-C6H3), −O(2,6-tBu2-4-Me-C6H2)) is reported. Protonation of the easily prepared anionic Ni(I) bis(amido) complex K{Ni[N(SiMe3)(2,6-iPr-C6H3)]2} in the presence of an appropriate L-type ligand results in loss of HN(SiMe3)(2,6-iPr-C6H3) and trapping of the resulting neutral Ni(I)-amido fragment to yield neutral, paramagnetic, two- and three-coordinate Ni(I) complexes. Protonation of these neutral amido complexes by the bulky phenol HO(2,6-tBu2-4-Me-C6H2) results in loss of the second amido moiety and trapping by the resulting phenoxide to yield Ni(I)-O(2,6-tBu2-4-Me-C6H2) complexes. The hapticity of the phenoxide ligand is influenced by the π-accepting ability of the L-type ligand. Where L = PtBu3, a poor π-acceptor, the phenoxide acts as a π-acceptor and adopts a η5-bonding mode through dearomatization of the phenyl ring. When L = NHC, a competent π-acceptor, the phenoxide acts as a π-donor, adopting a η1-bonding mode through the O atom. The modular nature of this synthetic strategy allows variation of both the L- and X-type ligands of the complex in a stepwise fashion and should be extendable to a wide variety of ligand types for new Ni(I) complexes. PMID:25328273

  8. [Cp(Ar)Ni{Ga(nacnac)}]: An Open-Shell Nickel(I) Complex Supported by a Gallium(I) Carbenoid (Cp(Ar) = C5(C6H4-4-Et)5, nacnac = HC[C(Me)N-(C6H3)-2,6-iPr2]2).

    PubMed

    Chakraborty, Uttam; Mühldorf, Bernd; van Velzen, Niels J C; de Bruin, Bas; Harder, Sjoerd; Wolf, Robert

    2016-03-21

    The 17 valence electron (VE) open-shell nickel gallanediyl complex [Cp(Ar)Ni{Ga(nacnac)}] (3, Ar = C5(C6H4-4-Et)5, nacnac = HC[C(Me)N(C6H3-2,6-iPr2)]2), having an unsupported Ni-Ga bond, was synthesized from [Cp(Ar)Ni(μ-Br)]2 (1) by reducing the adduct [Cp(Ar)Ni(μ-Br){Ga(nacnac)}] (2) or, alternatively, trapping the "Cp(Ar)Ni(I)" synthon with Ga(nacnac); spectroscopic and DFT studies showed that the single unpaired electron in 3 resides mainly at the Ni center.

  9. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  10. 78 FR 45535 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ..., Longwood, Florida; W. Andrew Krusen, Jr., Tampa, Florida; Allan S. Martin, Tampa, Florida; Linda C. McGurn..., Tampa, Florida; William Andrew Krusen, Jr. SEP IRA, Tampa, Florida; Krusen Limited Partnership,...

  11. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  12. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  13. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  14. Working Together, We Can Defeat Cancer

    MedlinePlus

    ... of this page please turn Javascript on. Dr. Andrew C. von Eschenbach, M.D. Director, National Cancer ... Photo courtesy of NIH/NCI An interview with Andrew C. von Eschenbach Cancer—in all its forms— ...

  15. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  16. Strength and Fatigue of NT551 Silicon Nitride and NT551 Diesel Exhaust Valves

    SciTech Connect

    Andrews, M.J.; Wereszczak, A.A.; Kirkland, T.P.; Breder, K.

    2000-02-01

    The content of this report is excerpted from Mark Andrew's Ph.D. Thesis (Andrews, 1999), which was funded by a DOEYOTT High Temperature Materials Laboratory Graduate Fellowship. It involves the characterization of NT551 and valves fabricated with it. Greater detail of the described issues may be found in that reference or through communications with Andrew Wereszczak.

  17. 78 FR 11676 - Notice of Inventory Completion: National Guard Bureau/A7AN, Air National Guard, Joint Base...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... Base Andrews, MD AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The National Guard Bureau, Air National Guard, Joint Base Andrews, MD, has completed an inventory of human remains and... National Guard, Joint Base Andrews, MD. Repatriation of the human remains to the Indian tribes stated...

  18. Alternative nucleophilic substrates for the endonuclease activities of human immunodeficiency virus type 1 integrase

    SciTech Connect

    Ealy, Julie B.; Sudol, Malgorzata; Krzeminski, Jacek; Amin, Shantu; Katzman, Michael

    2012-11-10

    Retroviral integrase can use water or some small alcohols as the attacking nucleophile to nick DNA. To characterize the range of compounds that human immunodeficiency virus type 1 integrase can accommodate for its endonuclease activities, we tested 45 potential electron donors (having varied size and number or spacing of nucleophilic groups) as substrates during site-specific nicking at viral DNA ends and during nonspecific nicking reactions. We found that integrase used 22 of the 45 compounds to nick DNA, but not all active compounds were used for both activities. In particular, 13 compounds were used for site-specific and nonspecific nicking, 5 only for site-specific nicking, and 4 only for nonspecific nicking; 23 other compounds were not used for either activity. Thus, integrase can accommodate a large number of nucleophilic substrates but has selective requirements for its different activities, underscoring its dynamic properties and providing new information for modeling and understanding integrase.

  19. The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase.

    PubMed

    Bauer, Robert J; Evans, Thomas C; Lohman, Gregory J S

    2016-01-01

    DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observed a significant substrate inhibition effect, as well as the inhibition of both the self-adenylylation and nick-sealing steps of T4 DNA ligase by non-nicked, non-substrate dsDNA. Inhibition by non-substrate DNA was dependent only on the total DNA concentration rather than the structure; with 1 μg/mL of 40-mers, 75-mers, or circular plasmid DNA all inhibiting ligation equally. A >15-fold reduction in T4 DNA ligase self-adenylylation rate when in the presence of high non-nicked dsDNA concentrations was observed. Finally, EMSAs were utilized to demonstrate that non-substrate dsDNA can compete with nicked dsDNA substrates for enzyme binding. Based upon these data, we hypothesize the inhibition of T4 DNA ligase by non-nicked dsDNA is direct evidence for a two-step nick-binding mechanism, with an initial, nick-independent, transient dsDNA-binding event preceding a transition to a stable binding complex in the presence of a nick site.

  20. The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase

    PubMed Central

    Bauer, Robert J.; Evans, Thomas C.; Lohman, Gregory J. S.

    2016-01-01

    DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observed a significant substrate inhibition effect, as well as the inhibition of both the self-adenylylation and nick-sealing steps of T4 DNA ligase by non-nicked, non-substrate dsDNA. Inhibition by non-substrate DNA was dependent only on the total DNA concentration rather than the structure; with 1 μg/mL of 40-mers, 75-mers, or circular plasmid DNA all inhibiting ligation equally. A >15-fold reduction in T4 DNA ligase self-adenylylation rate when in the presence of high non-nicked dsDNA concentrations was observed. Finally, EMSAs were utilized to demonstrate that non-substrate dsDNA can compete with nicked dsDNA substrates for enzyme binding. Based upon these data, we hypothesize the inhibition of T4 DNA ligase by non-nicked dsDNA is direct evidence for a two-step nick-binding mechanism, with an initial, nick-independent, transient dsDNA-binding event preceding a transition to a stable binding complex in the presence of a nick site. PMID:26954034

  1. Teaching and Learning Astronomy

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay; Percy, John

    2005-12-01

    Preface; Part I. Astronomy in the Curriculum Around the World: Preface; 1. Why astronomy is useful and should be included in the school curriculum John R. Percy; 2. Astronomy and mathematics education Rosa M. Ros; 3. Astronomy in the curriculum around the world; 4. Engaging gifted science students through astronomy Robert Hollow; 5. Poster highlights: astronomy in the curriculum around the world; Part II. Astronomy Education Research: Preface; 6. Astronomy education research down under John M. Broadfoot and Ian S. Ginns; 7. A contemporary review of K-16 astronomy education research Janelle M. Bailey and Timothy F. Slater; 8. Implementing astronomy education research Leonarda Fucili; 9. The Astronomy Education Review: report on a new journal Sidney C. Wolff and Andrew Fraknoi; 10. Poster highlights: astronomy education research; Part III. Educating Students: Preface; 11. Textbooks for K-12 astronomy Jay M. Pasachoff; 12. Distance/internet astronomy education David H. McKinnon; 13. Educating students with robotic telescopes - open discussion; 14. Poster highlights - educating students; Part IV. Educating teachers: Preface; 15. Pre-service astronomy education of teachers Mary Kay Hemenway; 16. In-service education of teachers Michèle Gerbaldi; 17. Poster highlights: educating teachers; Part V. Astronomy and Pseudoscience: Preface; 18. Astronomy, pseudoscience and rational thinking Jayant V. Narlikar; 19. Astronomical pseudosciences in North America John R. Percy and Jay M. Pasachoff; Part VI. Astronomy and Culture: Preface; 20. Teaching astronomy in other cultures: archeoastronomy Julieta Fierro; 21. Poster highlights: astronomy and culture; Part VII. Astronomy in Developing Countries: Preface; 22. Astronomy Curriculum for developing countries Case Rijsdijk; 23. Science education resources for the developing countries James C. White II; Part VIII. Public Outreach in Astronomy: Preface; 24. What makes informal education programs successful? Nahide Craig and Isabel

  2. Teaching and Learning Astronomy

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay; Percy, John

    2009-07-01

    Preface; Part I. Astronomy in the Curriculum Around the World: Preface; 1. Why astronomy is useful and should be included in the school curriculum John R. Percy; 2. Astronomy and mathematics education Rosa M. Ros; 3. Astronomy in the curriculum around the world; 4. Engaging gifted science students through astronomy Robert Hollow; 5. Poster highlights: astronomy in the curriculum around the world; Part II. Astronomy Education Research: Preface; 6. Astronomy education research down under John M. Broadfoot and Ian S. Ginns; 7. A contemporary review of K-16 astronomy education research Janelle M. Bailey and Timothy F. Slater; 8. Implementing astronomy education research Leonarda Fucili; 9. The Astronomy Education Review: report on a new journal Sidney C. Wolff and Andrew Fraknoi; 10. Poster highlights: astronomy education research; Part III. Educating Students: Preface; 11. Textbooks for K-12 astronomy Jay M. Pasachoff; 12. Distance/internet astronomy education David H. McKinnon; 13. Educating students with robotic telescopes - open discussion; 14. Poster highlights - educating students; Part IV. Educating teachers: Preface; 15. Pre-service astronomy education of teachers Mary Kay Hemenway; 16. In-service education of teachers Michèle Gerbaldi; 17. Poster highlights: educating teachers; Part V. Astronomy and Pseudoscience: Preface; 18. Astronomy, pseudoscience and rational thinking Jayant V. Narlikar; 19. Astronomical pseudosciences in North America John R. Percy and Jay M. Pasachoff; Part VI. Astronomy and Culture: Preface; 20. Teaching astronomy in other cultures: archeoastronomy Julieta Fierro; 21. Poster highlights: astronomy and culture; Part VII. Astronomy in Developing Countries: Preface; 22. Astronomy Curriculum for developing countries Case Rijsdijk; 23. Science education resources for the developing countries James C. White II; Part VIII. Public Outreach in Astronomy: Preface; 24. What makes informal education programs successful? Nahide Craig and Isabel

  3. 78 FR 66422 - Appointment of Members of the Legal Division to the Performance Review Board, Internal Revenue...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... (Small Business/Self Employed) 4. Curtis G. Wilson, Associate Chief Counsel (Passthroughs and Special Industries) 5. Andrew Keyso, Associate Chief Counsel (Income Tax & Accounting) This publication is...

  4. STS-134 Tribute to Endeavour

    NASA Video Gallery

    STS-134 Commander Mark Kelly pays tribute to space shuttle Endeavour and the spacecraft's contribution to human spaceflight. Mission specialists Andrew Feustel, Mike Fincke, Roberto Vittori, Greg C...

  5. 4. Historic American Buildings Survey J. Alexander, Photographer August 1968 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey J. Alexander, Photographer August 1968 BEDROOM FIREPLACE, THIRD FLOOR - Andrew Ross Tenant House II, 1210 Thirtieth Street Northwest, Washington, District of Columbia, DC

  6. 3. Historic American Buildings Survey J. Alexander, Photographer August 1968 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey J. Alexander, Photographer August 1968 SECOND FLOOR PARLOR FIREPLACE - Andrew Ross Tenant House II, 1210 Thirtieth Street Northwest, Washington, District of Columbia, DC

  7. Comment on “Detrital U-Pb zircon dating of lower Ordovician syn-arc-continent collision conglomerates in the Irish Caledonides” by Peter D. Clift, Andrew Carter, Amy E. Draut, Hoang Van Long, David M. Chew, Hans A. Schouten, Tectonophysics 479 (2009), 165-174 (doi:10.1016/j.tecto.2008.07.018)

    NASA Astrophysics Data System (ADS)

    McConnell, Brian; Riggs, Nancy; Crowley, Quentin G.

    2010-07-01

    Clift et al. [Clift, P.D., Carter, A., Draut, A.E., Van Long, H., Chew, D.M. & Schouten, H.A., 2009.Detrital U-Pb zircon dating of lower Ordovician syn-arc-continent collision conglomerates in the Irish Caledonides. Tectonophysics, 479, 165-174] present U-Pb detrital zircon age data for one sample from each of three formations of the Ordovician South Mayo Trough sedimentary sequence and interpret these in the context of a 'Taiwan model' of arc-continent collision on the Laurentian margin of Iapetus. Their interpretation relies on an overly simplistic treatment of their data and does not take cognisance of other data relevant to the relative time of deposition of the sampled horizons within the orogenic history and the evolving availability of sediment source rocks.

  8. IMPACT OF CRITICAL ANION SOIL SOLUTION CONCENTRATION ON ALUMINUM ACTIVITY IN ALPINE TUNDRA SOIL Andrew Evans, Jr.1 , Michael B. Jacobs2, and Jason R. Janke1, (1) Metropolitan State University of Denver, Dept. of Earth and Atmospheric Sciences, (2) Dept. of Chemistry, Denver, CO, United States.

    NASA Astrophysics Data System (ADS)

    Evans, A.

    2015-12-01

    Soil solution anionic composition can impact both plant and microbial activity in alpine tundra soils by altering biochemical cycling within the soil, either through base cation leaching, or shifts in aluminum controlling solid phases. Although anions play a critical role in the aqueous speciation of metals, relatively few high altitude field studies have examined their impact on aluminum controlling solid phases and aluminum speciation in soil water. For this study, thirty sampling sites were selected on Trail Ridge Road in Rocky Mountain National Park, Estes Park, CO, and sampled during July, the middle of the growing season. Sampling elevations ranged from approximately 3560 - 3710 m. Soil samples were collected to a depth of 15.24 cm, and the anions were extracted using a 2:1 D.I. water to soil ratio. Filtered extracts were analyzed using IC and ICP-MS. Soil solution NO3- concentrations were significantly higher for sampling locations east of Iceberg Pass (EIBP) (mean = 86.94 ± 119.8 mg/L) compared to locations west of Iceberg Pass (WIBP) (mean 1.481 ± 2.444 mg/L). Both F- and PO43- soil solution concentrations, 0.533 and 0.440 mg/L, respectively, were substantially lower, for sampling sites located EIBP, while locations WIBP averaged 0.773 and 0.829 mg/L respectively, for F- and PO43-. Sulfate concentration averaged 3.869 ± 3.059 mg/L for locations EIBP, and 3.891 ± 3.1970 for locations WIBP. Geochemical modeling of Al3+ in the soil solution indicated that a suite of aluminum hydroxyl sulfate minerals controlled Al3+ activity in the alpine tundra soil, with shifts between controlling solid phases occurring in the presence of elevated F- concentrations.

  9. United States History Simulations: 1787-1868: Constitution Convention, Missouri Compromise, Treaty of Guadalupe Hidalgo, The Compromise of 1850, The Kansas/Nebraska Act, Southern Secession from the Union, and the Impeachment and Trial of Andrew Johnson. ETC Simulations Number Two.

    ERIC Educational Resources Information Center

    Hostrop, Richard W.

    This book presents simulation activities for significant events in U.S. history from 1787-1868. Intended for student involvement, the simulations require student research and practice in order to carry out the designated roles. The simulation and role play serve to involve the students actively in their learning, using both the affective and…

  10. Comment on "Nanosecond laser textured superhydrophobic metallic surfaces and their chemical sensing applications" by Duong V. Ta, Andrew Dunn, Thomas J. Wasley, Robert W. Kay, Jonathan Stringer, Patrick J. Smith, Colm Connaughton, Jonathan D. Shephard (Appl. Surf. Sci. 357 (2015) 248-254)

    NASA Astrophysics Data System (ADS)

    Boinovich, L. B.; Emelyanenko, A. M.; Emelyanenko, K. A.; Domantovsky, A. G.; Shiryaev, A. A.

    2016-08-01

    Nowadays the problem of design of durable ecologically friendly superhydrophobic surfaces is of great importance for science and technology. A recent paper in Applied Surface Science reports the method of fabricating the superhydrophobic metallic surfaces by infrared nanosecond laser surface texturing without using hydrophobic agents. Since this method of surface texturing can be considered as one of the most suitable for various industrial applications, the nature of superhydrophobic state of surfaces produced by laser texturing in the abovementioned paper deserves to be analyzed in detail. Authors of the commented paper attributed the change in wettability to the partial deoxidation of CuO into Cu2O on the surface during storage in atmosphere. However, such interpretation of the results contradicts to the basic notions in the theory of wetting and to more accurate and detailed data. In our Comment we discuss these contradictions point by point.

  11. Accentuating the Positive: "Can't" Doesn't Cut It

    ERIC Educational Resources Information Center

    Hyndman, Amy

    2011-01-01

    Nick is the author's first-born child. He was born healthy in 1993. Within days, however, there would be medical complications resulting in brain damage, seizures, medical issues, a diagnosis of cerebral palsy at four months of age, and later, a diagnosis of bi-lateral progressive hearing loss at eight years of age. Nick's parents have been told…

  12. Repair synthesis step involving ERCC1-XPF participates in DNA repair of the Top1-DNA damage complex.

    PubMed

    Takahata, Chiaki; Masuda, Yuji; Takedachi, Arato; Tanaka, Kiyoji; Iwai, Shigenori; Kuraoka, Isao

    2015-08-01

    Topoisomerase 1 (Top1) is the intercellular target of camptothecins (CPTs). CPT blocks DNA religation in the Top1-DNA complex and induces Top1-attached nick DNA lesions. In this study, we demonstrate that excision repair cross complementing 1 protein-xeroderma pigmentosum group F (ERCC1-XPF) endonuclease and replication protein A (RPA) participate in the repair of Top1-attached nick DNA lesions together with other nucleotide excision repair (NER) factors. ERCC1-XPF shows nuclease activity in the presence of RPA on a 3'-phosphotyrosyl bond nick-containing DNA (Tyr-nick DNA) substrate, which mimics a Top1-attached nick DNA lesion. In addition, ERCC1-XPF and RPA form a DNA/protein complex on the nick DNA substrate in vitro, and co-localize in CPT-treated cells in vivo. Moreover, the DNA repair synthesis of Tyr-nick DNA lesions occurred in the presence of NER factors, including ERCC1-XPF, RPA, DNA polymerase delta, flap endonuclease 1 and DNA ligase 1. Therefore, some of the NER repair machinery might be an alternative repair pathway for Top1-attached nick DNA lesions. Clinically, these data provide insights into the potential of ERCC1 as a biomarker during CPT regimens.

  13. E-Books Mediator: Nicholas Bogaty--Open Ebook Forum, New York

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    This article is about the work of Nick Bogaty, executive director of the Open eBook Forum. Nick Bogaty is not a librarian, but he plays nicely with them, along with publishers, hardware manufacturers, software producers, database vendors, and disability rights advocates. All are groups that share an interest in making e-books work for their…

  14. 78 FR 894 - Interim Final Determination To Stay Sanctions, Imperial County Air Pollution Control District

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... AGENCY 40 CFR Part 52 Interim Final Determination To Stay Sanctions, Imperial County Air Pollution... of revisions to the Imperial County Air Pollution Control District (ICAPCD) portion of the California...- line instructions. 2. Email: steckel.andrew@epa.gov . 3. Mail or deliver: Andrew Steckel (Air-4),...

  15. 76 FR 62002 - Revisions to the California State Implementation Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ...-0356, by one of the following methods: 1. Federal eRulemaking Portal: www.regulations.gov . Follow the on- line instructions. 2. E-mail: steckel.andrew@epa.gov . 3. Mail or deliver: Andrew Steckel (Air-4... should not be submitted through http://www.regulations.gov or e-mail. http://www.regulations.gov is...

  16. 78 FR 70295 - Intent to Prepare an Environmental Impact Statement for the Dam Safety Modification Report...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-25

    ... through the use of hydropower penstocks substantially reducing risk. However, physical modeling and expert... INFORMATION CONTACT: Andrew N. Johnson, Environmental Analysis Section, U.S. Army Corps of Engineers...: andrew.n.johnson@usace.army.mil . SUPPLEMENTARY INFORMATION: 1. Authority: Investigation...

  17. Huggins, martians and exoplanets

    NASA Astrophysics Data System (ADS)

    2013-02-01

    The RAS Ordinary A & G meeting in November included the Gerald Whitrow Lecture by Prof. Andrew Liddle, while in December Prof. Andrew Collier Cameron gave the George Darwin Lecture. Both will be reported in future issues. Here Sue Bowler summarizes the other talks.

  18. ES Review: Selections from 2009 and 2010

    ERIC Educational Resources Information Center

    Smiles, Robin, Ed.

    2010-01-01

    This fourth edition of the "ES Review" brings together, in one setting, some of the best work from 2009-10. It features: (1) Teacher Quality (Teachers at Work: Improving Teacher Quality Through School Design (Elena Silva); Understanding Teachers Contracts (Andrew J. Rotherham); How Teachers Unions Lost the Media (Richard Whitmire and Andrew J.…

  19. Presidents Talking about the Presidency.

    ERIC Educational Resources Information Center

    Twohig, Dorothy; And Others

    1997-01-01

    Presents brief excerpts from the presidential papers of George Washington, Thomas Jefferson, Andrew Jackson, James Polk, Andrew Johnson, Ulysses S. Grant, and Dwight D. Eisenhower. The excerpts address such matters as voting rights, limited government, political appointments, national security, and the balance of power. (MJP)

  20. Algebra: How Is It for You?

    ERIC Educational Resources Information Center

    Rickard, Caroline

    2008-01-01

    Shortly after starting work for the University of Chichester in the School of Teacher Education, the author was planning a session relating to algebra and found herself inspired by an article in MT182: "Algebraic Infants" by Andrews and Sayers (2003). Based on the making of families of "Multilink" animals, Andrews and Sayers (2003) claim that…

  1. Argument Reconceived?

    ERIC Educational Resources Information Center

    Coffin, Caroline; O'Halloran, Kieran A.

    2009-01-01

    Just over 10 years ago, "Educational Review" published an article "Reconceiving argument" by Richard Andrews. In the article, Andrews traced some of the changes in the conception of argument that had taken place within educational contexts (primarily within the UK) over the previous few years. An important aim of the authors' article is to…

  2. Analysis of the Latin Square Task with Linear Logistic Test Models

    ERIC Educational Resources Information Center

    Zeuch, Nina; Holling, Heinz; Kuhn, Jorg-Tobias

    2011-01-01

    The Latin Square Task (LST) was developed by Birney, Halford, and Andrews [Birney, D. P., Halford, G. S., & Andrews, G. (2006). Measuring the influence of cognitive complexity on relational reasoning: The development of the Latin Square Task. Educational and Psychological Measurement, 66, 146-171.] and represents a non-domain specific,…

  3. The Evolution of an Adolescent Literacy Program: A Foundation's Journey

    ERIC Educational Resources Information Center

    Henriquez, Andres

    2005-01-01

    The Carnegie Corporation of New York is associated with the very foundations of literacy going back to the philanthropy of Andrew Carnegie himself and of the Corporation in its early years. Andrew Carnegie's legacy includes over 2,500 free public libraries that he saw as a link "bridging ignorance and education." In fact, Carnegie, the man,…

  4. Creating a Shared Definition of Good and Bad Writing through Revision Strategies

    ERIC Educational Resources Information Center

    Baer, Allison L.

    2008-01-01

    This article describes how the author, in an effort to build some common language about "good writing" and to teach effective revision strategies, involved her students in an action research project using the Worse/Better Writing Strategy developed by Andrews-Beck. In her dissertation, Andrews-Beck described a worse/better writing strategy that…

  5. Technology Counts 2007: A Digital Decade

    ERIC Educational Resources Information Center

    Education Week, 2007

    2007-01-01

    "Technology Counts 2007" looks back, and ahead, after a decade of enormous upheaval in the educational technology landscape. This special issue of "Education Week" includes the following articles: (1) A Digital Decade; (2) Getting Up to Speed (Andrew Trotter); (3) E-Rate's Imprint Seen in Schools (Andrew Trotter); (4) Teaching Assistants (Rhea R.…

  6. 78 FR 12346 - Jennings Low-Effect Habitat Conservation Plan for the Morro Shoulderband Snail, Community of Los...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ... December 15, 1994 (59 FR 64613). Section 9 of the Endangered Species Act of 1973, as amended (Act) and its... Service, have received an application from Andrew A. Jennings for a 10-year incidental take permit under... animal or plant species. Andrew A. Jennings (hereafter, the applicant) has submitted a Low-...

  7. Effective Conditions for Learning

    ERIC Educational Resources Information Center

    Ball, Barbara; Ball, Derek

    2007-01-01

    In this article, the authors respond to four 2006 "Mathematics Teaching" articles by Paul Andrews and Judy Sayers comparing conditions for learning mathematics in England and in four other European countries. They reflect on what might be done with English education, and with English mathematics education in particular. They challenge Andrews' and…

  8. Why Play "I spy" When You Can Do Mathematics?

    ERIC Educational Resources Information Center

    Andrews, Robert; Andrews, Paul

    2007-01-01

    This article presents a story of Robert Andrew's mental engagement with mathematics. It has been jointly written by Robert, who is a Y8 student, and his father, Paul Andrews, who is a mathematics educator. It starts with Robert who presents the context for the story and is followed by Paul's commentary which draws on what Robert has written.…

  9. Predicting mangrove forest recovery on the southwest coast of Florida following the impact of Hurricane Wilma, October 2005: Chapter 6H in Science and the storms-the USGS response to the hurricanes of 2005

    USGS Publications Warehouse

    Ward, Greg A.; Smith, Thomas J.

    2007-01-01

    The damage to mangrove forests on the west coast of Everglades National Park from Hurricane Wilma in 2005 rivaled that of Hurricane Andrew in 1992. We describe patterns and rates of recovery following Andrew and use these estimates to gage recovery based upon site reconnaissance and forest structural damage considerations in the aftermath of Wilma.

  10. An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata.

    PubMed

    Begam, M; Narwal, S; Roy, S; Kumar, S; Lodha, M L; Kapoor, H C

    2006-01-01

    An antiviral protein named CCP-27 was purified from the leaves of Celosia cristata at the post-flowering stage by anion-exchange, cation-exchange, and gel-filtration chromatography. It exhibited resistance against sunnhemp rosette virus in its test host Cyamopsis tetragonoloba. It also exhibited deoxyribonuclease activity against supercoiled pBlueScript SK+ plasmid DNA. It was found to nick supercoiled DNA into nicked circular form at lower protein concentration followed by nicked to linear form conversion at higher protein concentration. CCP-27 also possesses strong ribonuclease activity against Torula yeast rRNA. PMID:16487067

  11. Learners in Action, Winter 2005

    ERIC Educational Resources Information Center

    Movement for Canadian Literacy, 2005

    2005-01-01

    This Winter 2005 issue of "Learners in Action" contains the following: (1) Proud Dad Turns Family Life Around (Nick Prince); (2) Learners Make a Great Impression at Conference; (3) The Story behind the Story; and (4) Learner Resources.

  12. In vitro packaging of phage DNA with defined lesions

    SciTech Connect

    Lee, E.; Hays, J.B.

    1986-05-01

    In vitro packaging of undamaged phage lambda DNA at efficiencies in excess of 2% has been routinely achieved using slightly modified standard techniques. In order to test the ability of nicks and gaps to stimulate genetic recombination during homoimmune and lytic infections, DNA was randomly nicked by treatment with methidium-propyl-EDTA-Fe(II) (MPE), and the nicks expanded to gaps with exonuclease III. DNA with about 15 MPE nicks per lambda duplex or the same number of 30-nucleotide gaps was packaged with slightly reduced efficiency. Surprisingly, DNA irradiated at 254 nm, containing 50 cyclobutane pyrimidine dimers per lambda duplex (plus other photoproducts) was packaged as efficiently as unirradiated DNA. Thus, phage containing only minor 254 nm photoproducts can be prepared efficiently by irradiation, enzymatic photoreactivation, and packaging.

  13. 76 FR 9071 - Advisory Committee on Historical Diplomatic Documentation; Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-16

    ... the public planning to attend must notify Nick Sheldon, Office of the Historian (202-663-1123) no..., Washington, DC, 20520, telephone (202) 663-1123, (e-mail history@state.gov ). Dated: February 9, 2011....

  14. A multiverse play divides opinion

    NASA Astrophysics Data System (ADS)

    Crease, Robert P.

    2015-03-01

    The stage lights rise. A man and woman meet in a cute way - "Do you know why it's impossible to lick the tips of your elbows?" she asks - they chat momentarily, and separate. The play is Constellations by Nick Payne.

  15. Hurricane impacts on the coastal environment

    USGS Publications Warehouse

    Sallenger, Abby

    In terms of insured losses, Hurricane Andrew is the most severe catastrophe in the Nation's history. Prior to the arrival of Andrew, the U.S. Geological Survey (USGS), in cooperation with the Louisiana Geological Survey (LGS), acquired an extensive body of information and data on the behavior and long-term erosion of Louisiana barrier islands. As a result, we have a clear understanding of pre-storm conditions in this area; Andrew provided an opportunity to learn in detail the impact of a very large storm on Louisiana coastal environment.

  16. Hurricane impacts on the coastal environment

    USGS Publications Warehouse

    Sallenger, Abby

    1990-01-01

    In terms of insured losses, Hurricane Andrew is the most severe catastrophe in the Nation's history. Prior to the arrival of Andrew, the U.S. Geological Survey (USGS), in cooperation with the Louisiana Geological Survey (LGS), acquired an extensive body of information and data on the behavior and long-term erosion of Louisiana barrier islands. As a result, we have a clear understanding of pre-storm conditions in this area; Andrew provided an opportunity to learn in detail the impact of a very large storm on Louisiana coastal environment.

  17. "Who's There?": Shakespeare and the Dragon of Autism

    ERIC Educational Resources Information Center

    Renino, Christopher

    2009-01-01

    Dan recently turned 13; Nick will soon be 12. Both boys are smart and fun; both are loving, write like angels, like to learn, and want friends; both are autistic and hope to learn to speak. They find it challenging to relate to and function in this world, and they work hard to improve their abilities to do so. Last fall, Nick and Dan became…

  18. Themes and variation of N. S. Thompson--song.

    PubMed

    Abbey, Emily

    2010-12-01

    Nick Thompson studied many animals over the course of his career, including non-human primates, dogs, crows, human babies, and mockingbirds. Amidst such variation, Nick maintained a common focus. He sought to provide more accurate and truthful representations of the particular phenomenon of interest. His writings on mentalism, design, anthropomorphism and use of metaphor have provided fellow scientists with insight and helped advance his field of study.

  19. A novel method to convert a DNA fragment inserted into a plasmid to an inverted repeat structure.

    PubMed

    Tomimoto, Kazuya; Fujita, Kosuke; Ishibashi, Jun; Imanishi, Shigeo; Yamakawa, Minoru; Tanaka, Hiromitsu

    2012-01-01

    Transfection of an expression plasmid possessing inverted repeat (IR) DNA into cultured cells leads to the overexpression of hairpin RNA and efficient suppression of target gene expression. Such DNA vector-based RNA interference (RNAi) is widely used for characterizing genes of interest in cultured cell lines. In this study, we developed a new method to convert an inserted DNA fragment (IDF) in specially designed plasmid vectors into an IR structure by using nicking endonucleases and BcaBEST DNA polymerase. This method consists of the following steps: (1) linearization of the plasmid with a nick by using a restriction enzyme and a nicking endonuclease, (2) formation of the hairpin-loop DNA at the end near the IDF of the linearized plasmid, (3) insertion of a nick at the other end of the IDF by a nicking endonuclease, (4) execution of the strand displacement reaction from the nick to synthesize IR DNA, and (5) self-ligation of the linear double-stranded DNA. The IR DNA containing expression plasmids constructed by this method effectively induced target-specific RNAi in a silkworm cell line. We further established a method to purify expression plasmids containing IR DNA. Our new methods provide techniques for the construction of long hairpin RNA (lhRNA) expression plasmids for silencing specific genes in silkworms and other organisms, and offer a fundamental methodology for constructing an lhRNA expression library from a cDNA plasmid library. PMID:21516519

  20. A novel method to convert a DNA fragment inserted into a plasmid to an inverted repeat structure.

    PubMed

    Tomimoto, Kazuya; Fujita, Kosuke; Ishibashi, Jun; Imanishi, Shigeo; Yamakawa, Minoru; Tanaka, Hiromitsu

    2012-01-01

    Transfection of an expression plasmid possessing inverted repeat (IR) DNA into cultured cells leads to the overexpression of hairpin RNA and efficient suppression of target gene expression. Such DNA vector-based RNA interference (RNAi) is widely used for characterizing genes of interest in cultured cell lines. In this study, we developed a new method to convert an inserted DNA fragment (IDF) in specially designed plasmid vectors into an IR structure by using nicking endonucleases and BcaBEST DNA polymerase. This method consists of the following steps: (1) linearization of the plasmid with a nick by using a restriction enzyme and a nicking endonuclease, (2) formation of the hairpin-loop DNA at the end near the IDF of the linearized plasmid, (3) insertion of a nick at the other end of the IDF by a nicking endonuclease, (4) execution of the strand displacement reaction from the nick to synthesize IR DNA, and (5) self-ligation of the linear double-stranded DNA. The IR DNA containing expression plasmids constructed by this method effectively induced target-specific RNAi in a silkworm cell line. We further established a method to purify expression plasmids containing IR DNA. Our new methods provide techniques for the construction of long hairpin RNA (lhRNA) expression plasmids for silencing specific genes in silkworms and other organisms, and offer a fundamental methodology for constructing an lhRNA expression library from a cDNA plasmid library.

  1. Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching between Two DNA-Bound States

    SciTech Connect

    Cotner-Gohara, Elizabeth; Kim, In-Kwon; Hammel, Michal; Tainer, John A.; Tomkinson, Alan E.; Ellenberger, Tom

    2010-09-13

    Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a 'jackknife model' in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

  2. The Future of Theoretical Physics and Cosmology

    NASA Astrophysics Data System (ADS)

    Gibbons, G. W.; Shellard, E. P. S.; Rankin, S. J.

    2009-08-01

    Preface; List of contributors; 1. Introduction; Part I. Popular Symposium: 2. Our complex cosmos and its future Martin J. Rees; 3. Theories of everything and Hawking's wave function of the Universe James B. Hartle; 4. The problem of space-time singularities: implications for quantum gravity? Roger Penrose; 5. Warping spacetime Kip Thorne; 6. 60 years in a nutshell Stephen W. Hawking; Part II. Spacetime Singularities: 7. Cosmological perturbations and singularities George F. R. Ellis; 8. The quantum physics of chronology protection Matt Visser; 9. Energy dominance and the Hawking-Ellis vacuum conservation theorem Brandon Carter; 10. On the instability of extra space dimensions Roger Penrose; Part III. Black Holes: 11. Black hole uniqueness and the inner horizon stability problem Werner Israel; 12. Black holes in the real universe and their prospects as probes of relativistic gravity Martin J. Rees; 13. Primordial black holes Bernard Carr; 14. Black hole pair creation Simon F. Ross; 15. Black holes as accelerators Steven Giddings; Part IV. Hawking Radiation: 16. Black holes and string theory Malcolm Perry; 17. M theory and black hole quantum mechanics Joe Polchinski; 18. Playing with black strings Gary Horowitz; 19. Twenty years of debate with Stephen Leonard Susskind; Part V. Quantum Gravity: 20. Euclidean quantum gravity: the view from 2002 Gary Gibbons; 21. Zeta functions, anomalies and stable branes Ian Moss; 22. Some reflections on the status of conventional quantum theory when applied to quantum gravity Chris Isham; 23. Quantum geometry and its ramifications Abhay Ashtekar; 24. Topology change in quantum gravity Fay Dowker; Part VI. M Theory and Beyond: 25. The past and future of string theory Edward Witten; 26. String theory David Gross; 27. A brief description of string theory Michael Green; 28. The story of M Paul Townsend; 29. Gauged supergravity and holographic field theory Nick Warner; 30. 57 varieties in a NUTshell Chris Pope; Part VII. de Sitter Space

  3. 78 FR 69710 - Notice of Permits Issued Under the Antarctic Conservation Act of 1978

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-20

    ... Dahood, ACA Permit Officer, Division of Polar Programs, Rm. 755, National Science Foundation, 4201 Wilson... received. The permit was issued on November 7, 2013 to: Andrew Klein, Permit No. 2014-021. Nadene...

  4. UNDERSTANDING PATHWAYS OF TOXICITY: MAKING SENSE OF CHANGING SIGNALS

    EPA Science Inventory

    Title:
    Understanding Pathways of Toxicity: Making sense of changing signals
    Authors & affiliations:
    Sid Hunter, Maria Blanton, Edward Karoly, Ellen Rogers, Leonard Mole, Phillip Hartig, James Andrews. Reproductive Toxicology Division, National Health and Environmental Ef...

  5. 78 FR 59649 - Foreign-Trade Zone 83-Huntsville, Alabama, Application for Subzone, VF Jeanswear, Hackleburg...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... limit of FTZ 83. In accordance with the Board's regulations, Camille Evans of the FTZ Staff is... Camille Evans at Camille.Evans@trade.gov or at (202) 482-2350. Dated: September 19, 2013. Andrew...

  6. Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, et al, 2010 American Geophysical Union Poster Session

    DOE Data Explorer

    Ezra Zemach

    2010-01-01

    Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, Brigette Martini, Chet Lide, Darrick Boschmann, John DIlles, Andrew Meigs, 2010 Ormat Nevada, Zonge Geophysical, Oregon State University American Geophysical Union, Poster Session

  7. 2. VIEW OF MEDFORD SERVICE CENTER, INTERIOR OF COMPOUND SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF MEDFORD SERVICE CENTER, INTERIOR OF COMPOUND SHOWING BUILDING #2301, #2500, AND #2205 (L. TO R.), FACING SOUTHEAST. - Medford Service Center, 1319 McAndrews Road, Medford, Jackson County, OR

  8. 6. BUILDING #2205, INTERIOR VIEW OF UPPER STORAGE FLOOR ('ATTIC') ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. BUILDING #2205, INTERIOR VIEW OF UPPER STORAGE FLOOR ('ATTIC') SHOWING LIGHT ADMITTED BY MAIN BANK AND UPPERMOST WINDOWS, FACING SOUTHWEST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  9. 7. BUILDING #2205, INTERIOR VIEW OF UPPER STORAGE FLOOR ('ATTIC') ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. BUILDING #2205, INTERIOR VIEW OF UPPER STORAGE FLOOR ('ATTIC') SHOWING FREIGHT ELEVATOR, FACING WEST-NORTHWEST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  10. 3. VIEW OF MEDFORD SERVICE CENTER, INTERIOR OF COMPOUND SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW OF MEDFORD SERVICE CENTER, INTERIOR OF COMPOUND SHOWING BUILDINGS #2301, #2205, #2500, #2202, AND #2300 (L. TO R.), FACING SOUTHWEST. - Medford Service Center, 1319 McAndrews Road, Medford, Jackson County, OR

  11. 2. VIEW OF NORTH FRONT AND WEST SIDE OF BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF NORTH FRONT AND WEST SIDE OF BUILDING #2205 (AREA CONTAINING STORAGE AND EQUIPMENT SUPPLIES), FACING SOUTHEAST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  12. Breast skin and nipple changes

    MedlinePlus

    ... treatment. SCALY, FLAKING, ITCHY SKIN This is usually eczema or a bacterial or fungal infection. See your ... Atopic dermatitis, eczema, and noninfectious immunodeficiency disorders. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the Skin: Clinical ...

  13. Cherokee Removal and American Politics.

    ERIC Educational Resources Information Center

    Grinde, Donald.

    1987-01-01

    Presents a brief history of the Cherokee Nation, from its first contact with De Soto in 1540 through Andrew Jackson's presidency. Concludes that the Cherokee removal clearly illustrates the shallowness of Jacksonian democratic principles. (JDH)

  14. 3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING #2205 (AREA CONTAINING VEHICLE REPAIR SHOP AND VEHICLE MANAGEMENT OFFICE), FACING SOUTHWEST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  15. Inhaled Version of Parkinson's Drug May Help Keep Symptoms At Bay

    MedlinePlus

    ... Feigin is a neurologist at the Northwell Health Neuroscience Institute in Great Neck, N.Y. "Levodopa remains ... Foundation; Andrew Feigin, M.D., neurologist, Northwell Health's Neuroscience Institute, Great Neck, N.Y.; Oct. 12, 2016, ...

  16. 1. Historic American Buildings Survey Photograph by Harold BushBrown Printed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Photograph by Harold Bush-Brown Printed and Enlarged by L.D. Andrew May, 23, 1936 FRONT CORNER VIEW - Griffin-Mott House, Mott Street & Front Avenue, Columbus, Muscogee County, GA

  17. 11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT COLLECTORS AT TOP LEFT AND OVERHEAD TROLLEY WIRES FOR POWER TRANSMISSION TO DRIVE MOTORS. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  18. 77 FR 36607 - Office of Hazardous Materials Safety Notice of Applications for Modification of Special Permit

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-19

    ....302(a), permit to authorize the Frontera. 178.65(f)(2). manufacture and filling of additional pressure vessels for use as components of airbag safety systems. 15540-M Andrew Airways, Inc. 49 CFR...

  19. Dyshidrotic eczema

    MedlinePlus

    ... PA: Elsevier Saunders; 2014:chap 190. James WD, Berger TG, Elston DM. Atopic dermatitis, eczema, and noninfectious immunodeficiency disorders. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the ...

  20. Atopic dermatitis - self-care

    MedlinePlus

    ... MO: Elsevier Saunders; 2016:chap 5. James WD, Berger TG, Elston DM. Atopic dermatitis, eczema, and noninfectious immunodeficiency disordersIn: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the ...

  1. 7. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer November 6, 1936 STAIR HALL OFF ENTRANCE HALL (LOOKING SOUTH EAST). - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  2. 3. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer June 6, 1936 NORTH SIDE ELEVATION SHOWING CONNECTION OF 'L' TO REAR. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  3. 8. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer November 6, 1936 CARARA MARBLE MANTLE RIGHT FRONT OF PARLOR. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  4. 5. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer November 6, 1936 DETAIL OF COLUMN CAPITALS (CAST IRON), FRONT PORTICO (LOOKING NORTH EAST). - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  5. 2. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer June 6, 1936 SOUTHSIDE ELEVATION SHOWING PORCH AND 'L' TO REAR. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  6. 10. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer November 6, 1936 DETAIL OF DOOR AND TRIM (UNPAINTED) SERVICE WING. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  7. 4. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer June 6, 1936 DETAIL REAR ELEVATION (LOOKING SOUTH WEST). - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  8. 9. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer November 6, 1936 PLASTER CORNICE AND CEILING ORNAMENT RIGHT FRONT PARLOR. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  9. 1. Historic American Buildings Survey, W. Jeter Eason, Deputy District ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey, W. Jeter Eason, Deputy District Officer, Photographer June 6, 1936 DETAIL OF FRONT OR WEST ELEVATION. - Rattle & Snap, U.S. Route 43 (Andrew Jackson Highway), Columbia, Maury County, TN

  10. Computers, Electronic Networking and Education: Some American Experiences.

    ERIC Educational Resources Information Center

    McConnell, David

    1991-01-01

    Describes new developments in distributed educational computing at Massachusetts Institute of Technology (MIT, "Athena"), Carnegie Mellon University ("Andrew"), Brown University "Intermedia"), Electronic University Network (California), Western Behavioral Sciences Institute (California), and University of California, Irvine. Topics discussed…

  11. Mentoring in Medicine | NIH MedlinePlus the Magazine

    MedlinePlus

    ... this page please turn Javascript on. Mentoring In Medicine Past Issues / Summer 2013 Table of Contents Students ... got hands-on healthcare experience through Mentoring in Medicine (MIM). At front is Andrew Morrison, MIM vice ...

  12. 77 FR 52695 - National Sea Grant Advisory Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... Program Web site at http://www.seagrant.noaa.gov/leadership/advisory_board.html . DATES: The announced....seagrant.noaa.gov/leadership/advisory_board.html . Dated: August 24, 2012. Andrew Baldus, Acting...

  13. Webb Telescope Backplane Arrives at NASA Goddard Space Flight Center

    NASA Video Gallery

    Webb Telescope's Backplane arrived at Joint Base Andrews on Monday, August 24, 2015 aboard a U.S. Air Force C-5 cargo plane. The Backplane, inside the Space Telescope Transporter for Air Road and S...

  14. Duff Green, Independent Editor of a Party Press

    ERIC Educational Resources Information Center

    Ewing, Gretchen Garst

    1977-01-01

    Tells how Duff Green obtained editorial and financial control of the "United States' Telegraph"--the primary organ for promoting the 1828 election campaign of Andrew Jackson--and how he expanded the role of partisan journalism. (GW)

  15. 77 FR 54927 - Comment Request for Information Collection for Unemployment Insurance (UI) Benefit Accuracy...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... Employment and Training Administration Comment Request for Information Collection for Unemployment Insurance... written comments to Andrew Spisak, Office of Unemployment Insurance, Room S-4524, Employment and Training... UI benefit payments in three programs: State UI, Unemployment Compensation for Federal...

  16. The (nano) entrepreneur's dilemma

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2015-03-01

    Emerging technologies need to be developed responsibly if their benefits are to outweigh any potential risks. Yet do entrepreneurs really have the luxury of grappling with future consequences from the get-go, asks Andrew D. Maynard.

  17. DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE

    EPA Science Inventory

    DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE. JE Andrews, H Nichols, and ES Hunter. Reproductive Toxicology Division, NHEERL, USEPA, RTP, NC.

    Disinfection of drinking water with chlorine results in numerous chlorinated byprodu...

  18. Early Geologic Education in California--Berkeley and Stanford Show the Way.

    ERIC Educational Resources Information Center

    Norris, Robert M.

    1981-01-01

    Traces the early history of geological education in California universities, with emphasis upon programs at Berkeley and Stanford. Among the pioneers in the field were Joseph LeConte, Andrew C. Lawson, and John C. Branner. (WB)

  19. 47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. W. ANDREWS PATENT PATTERN DEVICE, C. 1870, VIEW LOOKING FROM THE FRONT, SECOND FLOOR SOUTH. - Watkins Mill, County Highway MM, Lawson, Ray County, MO

  20. 77 FR 67020 - Performance Review Board Appointments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-08

    ..., Michael Black, Michael Black, Steven Blanchard, Mary Josie Bolton, Hannibal Burden, John Burzyk, Carla..., Jerold Glenn, Douglas Gonzales-Schreiner, Roseann Gould, Gregory Graziano, Angela Gross, Lawrence Gunderson, Linda Haugrud, Kevin Hawbecker, Karen Ishee, Mary Katherine Iudicello, Fay Jackson, Andrew...

  1. 32 CFR Appendix A to Part 44 - Guidance

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Non-Federal employers of Ready Reservists, particularly in the fields of public health and safety and... Division 15303 Andrews Road Kansas City, MO 64147-1207 Air Force Reserve Commander Air Reserve...

  2. 32 CFR Appendix A to Part 44 - Guidance

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Non-Federal employers of Ready Reservists, particularly in the fields of public health and safety and... Division 15303 Andrews Road Kansas City, MO 64147-1207 Air Force Reserve Commander Air Reserve...

  3. 2nd Antibiotic Halves C-Section Infection Rate

    MedlinePlus

    ... gov/news/fullstory_161230.html 2nd Antibiotic Halves C-Section Infection Rate: Study Two medications are better ... delivery, Andrews said. Overall, about 12 percent of C-sections result in an infection, according to background ...

  4. Starting Over. Vocational Programs across the Country Play an Important Role in Retraining Workers.

    ERIC Educational Resources Information Center

    Mateen, Naima; And Others

    1993-01-01

    Includes "Laid-Off Workers Turn to Milwaukee's HIRE" (Mateen); "New Beginnings after Andrew" (Tate); "In Wisconsin, Uniroyal Workers Face New Reality" (Mortwedt); and "Washington Timber Workers Retrain for Technical Jobs" (Manspeaker, Krug). (JOW)

  5. 76 FR 70957 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Manufacturing Authority, Sub...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ... improve the plant's international competitiveness. In accordance with the Board's regulations, Pierre Duy... information, contact Pierre Duy at Pierre.Duy@trade.gov or (202) 482-1378. Dated: November 10, 2011. Andrew...

  6. Psychometric Characteristics of the Psychopathic Personality Inventory in a Dutch-Speaking Population.

    ERIC Educational Resources Information Center

    Maesschalck, Christine; Vertommen, Hans; Hooghe, An

    2002-01-01

    Administered the Dutch adaptation of the Psychopathic Personality Inventory (R. Lilienfeld and B. Andrews, 1996) to 314 adults in Flanders and studied the factorial structure and scale characteristics. Results support the usefulness of the instrument. (SLD)

  7. Towards Experimental Annotation of Genes by High Throughput Sequencing

    SciTech Connect

    Bradbury, Andrew

    2010-06-03

    Andrew Bradbury of Los Alamos National Laboratory discusses turning annotation into a sequencing pipeline on June 3, 2010 at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  8. 76 FR 24831 - Site-Specific Analyses for Demonstrating Compliance With Subpart C Performance Objectives

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ... Programs, U.S. Nuclear Regulatory Commission, Washington, DC 20555-0001, telephone 301-415- 1078, e-mail...) 415- 1078 or e-mail Andrew.Carrera@nrc.gov of their planned attendance and if special services...

  9. Advanced Materials and Solids Analysis Research Core (AMSARC)

    EPA Science Inventory

    The Advanced Materials and Solids Analysis Research Core (AMSARC), centered at the U.S. Environmental Protection Agency's (EPA) Andrew W. Breidenbach Environmental Research Center in Cincinnati, Ohio, is the foundation for the Agency's solids and surfaces analysis capabilities. ...

  10. 76 FR 40700 - Marine Mammals; File No. 15014-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... Part 216). The original permit (No. 15014), issued on September 2, 2010 (75 FR 55307) authorized import... Loop, Suite 400, Orlando, FL 32819 [Brad Andrews, Responsible Party] has been issued a minor...

  11. 78 FR 73824 - Foreign-Trade Zone (FTZ) 20-Suffolk, Virginia; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    .... CVI already has authority to produce a range of printers, copiers and their parts, including toner..., contact Diane Finver at Diane.Finver@trade.gov or (202) 482-1367. Dated: December 3, 2013. Andrew...

  12. Interior view at top of parachute drying tower showing original ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view at top of parachute drying tower showing original steel wall-mounted ladder, facing southwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  13. Oblique view of south and east sides showing parachute tower, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of south and east sides showing parachute tower, facing northwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  14. Oblique view of north side showing bracketed window awnings, main ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of north side showing bracketed window awnings, main entry, and mission coping, facing southeast. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  15. Interior view of main entry hall showing stairs and original ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of main entry hall showing stairs and original pipe handrails, facing north. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  16. General view of building 405 in context showing hangars in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of building 405 in context showing hangars in distance, facing west. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  17. Detail of parachute tower showing integration with main roof form, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of parachute tower showing integration with main roof form, facing southwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  18. Detail of southwest corner showing rear double door entry, bracketed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of southwest corner showing rear double door entry, bracketed window awnings, and decorative parapet coping, facing northeast. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  19. 76 FR 20757 - Self-Regulatory Organizations; C2 Options Exchange, Incorporated; Notice of Designation of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... CFR 240.19b-4. \\3\\ See Securities Exchange Act Release No. 64011 (March 2, 2011), 76 FR 12775..., Commission, dated March 18, 2011; letter from Andrew Stevens, Legal Counsel, IMC Chicago, LLC, to Elizabeth...

  20. 76 FR 55061 - Two Proposed CERCLA Administrative Settlement Agreements for Long-Term Access at the Bountiful...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... Wynkoop Street, Denver, Colorado 80202-1129. Ms. Pokorny can be reached at (303) 312-6970. Comments should... Street, Denver, Colorado 80202-1129. Telephone: (303) 312-6970. Dated: August 26, 2011. Andrew M....

  1. 78 FR 13857 - Foreign-Trade Zone 93-Raleigh-Durham, NC; Authorization of Production Activity; Revlon Consumer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... (77 FR 65856-65857, 10/31/12). The FTZ Board has determined that no further review of the activity is... FTZ Act and the Board's regulations, including Section 400.14. Dated: February 19, 2013. Andrew...

  2. 99. Historic American Buildings Survey, Donald W. Dickensheets, Photographer. March ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    99. Historic American Buildings Survey, Donald W. Dickensheets, Photographer. March 29, 1940. ST. ANDREW. (SOUTHEAST ELEVATION). HIGH ALTAR - San Xavier del Bac Mission, Mission Road, Tucson, Pima County, AZ

  3. 78 FR 16246 - Reorganization of Foreign-Trade Zone 72 (Expansion of Service Area) Under Alternative Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... Federal Register (77 FR 59373-69374, 9/27/2012) and the application has been processed pursuant to the FTZ... Administration, Alternate Chairman, Foreign-Trade Zones Board. Andrew McGilvray, Executive Secretary....

  4. 78 FR 7394 - Foreign-Trade Zone 121-Albany, NY; Authorization of Production Activity; Albany Molecular...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ... notice in the Federal Register inviting public comment (77 FR 63290, 10/16/2012). The FTZ Board has... Section 400.14. Dated: January 25, 2013. Andrew McGilvray, Executive Secretary. BILLING CODE P...

  5. 76 FR 35221 - Epidemiology Program for American Indian/Alaska Native Tribes and Urban Indian Communities...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... June 8, 2011, in FR Doc. 2011-14131, on page 33318, in the first column, last complete sentence in the... of Grants Management to the attention of Andrew Diggs by June 17, 2011.'' Dated: June 9, 2011....

  6. 33 CFR 100.1308 - Special Local Regulation; Hydroplane Races within the Captain of the Port Puget Sound Area of...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... event in accordance with 33 CFR 100. The Captain of the Port will provide notice of the enforcement of... north of Andrew's Bay to include all waters east of the shoreline within the following points:...

  7. 75 FR 11516 - Marine Mammals; File No. 808-1735

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-11

    .... 808-1735, issued on June 27, 2007 (72 FR 36429), authorizes the permit holder to take humpback.... ACTION: Notice; receipt of application for amendment. SUMMARY: Notice is hereby given that Andrew...

  8. 33 CFR 100.1308 - Special Local Regulation; Hydroplane Races within the Captain of the Port Puget Sound Area of...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... event in accordance with 33 CFR 100. The Captain of the Port will provide notice of the enforcement of... north of Andrew's Bay to include all waters east of the shoreline within the following points:...

  9. 78 FR 59649 - Subzone 26G, Authorization of Production Activity, Roper Corporation, (Kitchen Ranges), Lafayette...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... 400), including notice in the Federal Register inviting public comment (78 FR 33809, June 5, 2013... regulations, including Section 400.14. Dated: September 18, 2013. Andrew McGilvray, Executive...

  10. 33 CFR 100.1308 - Special Local Regulation; Hydroplane Races within the Captain of the Port Puget Sound Area of...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... event in accordance with 33 CFR 100. The Captain of the Port will provide notice of the enforcement of... north of Andrew's Bay to include all waters east of the shoreline within the following points:...

  11. 75 FR 15413 - Approval for Processing Authority, Foreign-Trade Zone 196, ATC Logistics & Electronics (Personal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    .../2009); Whereas, notice inviting public comment has been given in the Federal Register (74 FR 49364, 9... Administration, Alternate Chairman, Foreign-Trade Zones Board. Attest: Andrew McGilvray, Executive...

  12. 18 CFR 1304.2 - Application.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... determined in accordance with 18 CFR part 1310, and disclosed to the applicant in the materials provided with..., Blue Ridge, Nottely, Ocoee; (4) 2611 W. Andrew Johnson Hwy., Morristown, TN 37814-3295, (865)...

  13. 33 CFR 100.1308 - Special Local Regulation; Hydroplane Races within the Captain of the Port Puget Sound Area of...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... hydroplane event in accordance with 33 CFR 100. The Captain of the Port will provide notice of the... north of Andrew's Bay to include all waters east of the shoreline within the following points:...

  14. 18 CFR 1304.2 - Application.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... determined in accordance with 18 CFR part 1310, and disclosed to the applicant in the materials provided with..., Blue Ridge, Nottely, Ocoee; (4) 2611 W. Andrew Johnson Hwy., Morristown, TN 37814-3295, (865)...

  15. 1. Historic American Buildings Survey J. Alexander, Photographer February 1969 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey J. Alexander, Photographer February 1969 EAST ELEVATIONS OF 1208-10 30th STREET - Andrew Ross Tenant House II, 1210 Thirtieth Street Northwest, Washington, District of Columbia, DC

  16. 78 FR 48647 - Foreign-Trade Zone 225-Springfield, Missouri; Authorization of Production Activity; General...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ...), including notice in the Federal Register inviting public comment (78 FR 22512, 4-16-2013). The FTZ Board has... Section 400.14. Dated: August 1, 2013. Andrew McGilvray, Executive Secretary. BILLING CODE 3510-DS-P...

  17. 78 FR 33808 - Foreign-Trade Zone 50-Long Beach, California; Authorization of Production Activity; Panasonic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... in the Federal Register inviting public comment (78 FR 9667, 2-11-2013). The FTZ Board has determined... 400.14. Dated: May 30, 2013. Andrew McGilvray, Executive Secretary. BILLING CODE 3510-DS-P...

  18. 77 FR 72816 - Foreign-Trade Zone 20-Suffolk, VA; Authorization of Production Activity; Usui International...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-06

    ... 400), including notice in the Federal Register inviting public comment (77 FR 48127-48128, 8-13-2012... regulations, including Section 400.14. Dated: November 30, 2012. Andrew McGilvray, Executive...

  19. 78 FR 30862 - Foreign-Trade Zone 41-Milwaukee, Wisconsin; Authorization of Production Activity; CNH America...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ... comment (78 FR 7394, 02-01-2013). The FTZ Board has determined that no further review of the activity is... FTZ Act and the Board's regulations, including Section 400.14. Dated: May 17, 2013. Andrew...

  20. Hot tub folliculitis

    MedlinePlus

    James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the Skin: Clinical Dermatology. 11th ed. Philadelphia, PA: Elsevier Saunders; 2011:chap 14. Pasternack MS, Swartz MN. Cellulitis, necrotizing fasciitis, and ...

  1. 2. Historic American Buildings Survey J. Alexander, Photographer August 1968 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey J. Alexander, Photographer August 1968 SECOND FLOOR PARLOR LOOKING NORTHWEST - Andrew Ross Tenant House II, 1210 Thirtieth Street Northwest, Washington, District of Columbia, DC

  2. 78 FR 20887 - Approval of Subzone Status; Pepsi Cola Puerto Rico Distributing, LLC, Toa Baja, Puerto Rico

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-08

    ..., including notice in the Federal Register inviting public comment (77 FR 74170, 12/13/2012). The FTZ staff....13, and further subject to FTZ 7's 2,000-acre activation limit. Dated: April 2, 2013. Andrew...

  3. 75 FR 17125 - Foreign-Trade Zone 113-Ellis County, Texas, Application for Reorganization under Alternative Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-05

    ... (75 FR 3705, 1/22/2010), is being extended to April 29, 2010 to allow interested parties additional....gov or (202) 482-2350. Dated: March 25, 2010. Andrew McGilvray, Executive Secretary. BILLING CODE...

  4. 76 FR 28418 - Voluntary Termination of Foreign-Trade Subzone 33C; Sony Corporation of America, Mt. Pleasant, PA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... the Sony Corporation of America plant in Mt. Pleasant, Pennsylvania (Board Order 1196, 66 FR 52741, 10..., Alternate Chairman, Foreign-Trade Zones Board. Andrew McGilvray, Executive Secretary. BILLING CODE P...

  5. Autumn MIST 2009

    NASA Astrophysics Data System (ADS)

    Forsyth, Colin

    2010-02-01

    MEETING REPORT Colin Forsyth, Nicola Longden, Andrew Walsh and Robert Wicks summarize a MIST meeting where ground-based ionospheric science came under the spotlight, amid the broader concerns of the MIST community.

  6. Magnetotails throughout the solar system

    NASA Astrophysics Data System (ADS)

    Forsyth, C.; Milan, S. E.; Walsh, A. P.

    2010-12-01

    MEETING REPORT A key part of heliospheric and planetary physics came under scrutiny at an RAS Specialist Discussion Meeting in October. Colin Forsyth, Chris Arridge, Steve Milan and Andrew Walsh summarize.

  7. Old materials, new challenges?

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2014-09-01

    Fumed silica has been used as an anti-caking agent in foods for several decades. Does new research suggest that the use of this engineered nanomaterial needs to be re-examined, asks Andrew D. Maynard.

  8. 76 FR 52670 - 2011 Technology Transfer Summit North America Conference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ..., JPMorgan --Maarten deJong, Managing Director, Barclays Capital --Andrew Robertson, Chief Policy Officer... College --Daniel Perez, Partner, Bay City Capital --Hubert Birner, Partner, TVM Capital --Glen Steinbach.... Ferguson, Deputy Director, Licensing & Entrepreneurship, Office of Technology Transfer, National...

  9. NCI Director Also to Be Interim FDA Commissioner

    Cancer.gov

    Andrew von Eschenbach, M.D., director of the NCI, was asked by President Bush on Friday, September 23, 2005, to assume the additional role of interim Commissioner of the U.S. Food and Drug Administration (FDA).

  10. 2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 FACING NORTHWEST. NOTE CURVED KNEE-BRACES ON SUPPORT POSTS. - Medford Service Center, Covered Storage Building, 1319 McAndrews Road, Medford, Jackson County, OR

  11. 8. Historic American Buildings Survey From photograph from Misses BaberBlackshear, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey From photograph from Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 ARCH AND STAIRHALL ON SECOND FLOOR - Baber House, Walnut Street, Macon, Bibb County, GA

  12. 1. Historic American Buildings Survey From photograph furnished by Misses ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey From photograph furnished by Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 FRONT ELEVATION - Baber House, Walnut Street, Macon, Bibb County, GA

  13. 7. Historic American Buildings Survey From Photograph furnished by Misses ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey From Photograph furnished by Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 MARBLE MANTEL IN PARLOR (Right Front Room) - Baber House, Walnut Street, Macon, Bibb County, GA

  14. Co-creating health's lively, moving frontiers: brief observations on the facets and possibilities of non-representational theory.

    PubMed

    Andrews, Gavin J

    2014-11-01

    This paper follows up a recent debate on the potential of non-representational theory in health geography (see Andrews et al 2014; Kearns 2014; Hanlon 2014; Andrews 2014c) by outlining, more broadly, some of its key facets and investigative possibilities. The intention is to provide a helpful introduction, and entry point, to this particular way of understanding the world and of going about research that might help scholars reverberate the 'taking place' of health and health care.

  15. The knowledge of our knowledge☆

    PubMed Central

    McAndrews, Jerome F.

    2012-01-01

    This classic article was published in the first volume and issue of Philosophical Constructs for the Chiropractic Profession. In this paper, Dr. McAndrews reviews the use of the term “philosophy” in chiropractic and urges the chiropractic profession to consider the use and misuse of this term. Reprinted with permission from McAndrews JF. The Knowledge of Our Knowledge. Philosophical Constructs for the Chiropractic Profession. 1991;1:14-17. PMID:23966887

  16. Effects of cargo molecules on membrane perturbation caused by transportan10 based cell-penetrating peptides.

    PubMed

    Vasconcelos, Luís; Madani, Fatemeh; Arukuusk, Piret; Pärnaste, Ly; Gräslund, Astrid; Langel, Ulo

    2014-12-01

    Cell-penetrating peptides with the ability to escape endosomes and reach the target are of great value as delivery vectors for different bioactive cargoes and future treatment of human diseases. We have studied two such peptides, NickFect1 and NickFect51, both originated from stearylated transportan10 (PF3). To obtain more insight into the mechanism(s) of peptide delivery and the biophysical properties of an efficient vector system, we investigated the effect of different bioactive oligonucleotide cargoes on peptide-membrane perturbation and peptide structural induction. We studied the membrane interactions of the peptides with large unilamellar vesicles and compared their effects with parent peptides transportan10 and PF3. In addition, cellular uptake and peptide-mediated oligonucleotide delivery were analyzed. Calcein leakage experiments showed that similar to transportan10, NickFect51 caused a significant degree of membrane leakage, whereas NickFect1, similar to PF3, was less membrane perturbing. The results are in agreement with previously published results indicating that NickFect51 is a more efficient endosomal escaper. However, the presence of a large cargo like plasmid DNA inhibited NickFect's membrane perturbation and cellular uptake efficiency of the peptide was reduced. We conclude that the pathway for cellular uptake of peptide complexes is cargo dependent, whereas the endosomal escape efficacy depends on peptide hydrophobicity and chemical structure. For small interfering RNA delivery, NickFect51 appears to be optimal. The biophysical signature shows that the peptide alone causes membrane perturbation, but the cargo complex does not. These two biophysical characteristics of the peptide and its cargo complex may be the signature of an efficient delivery vector system.

  17. Dual daughter strand incision is processive and increases the efficiency of DNA mismatch repair.

    PubMed

    Hermans, Nicolaas; Laffeber, Charlie; Cristovão, Michele; Artola-Borán, Mariela; Mardenborough, Yannicka; Ikpa, Pauline; Jaddoe, Aruna; Winterwerp, Herrie H K; Wyman, Claire; Jiricny, Josef; Kanaar, Roland; Friedhoff, Peter; Lebbink, Joyce H G

    2016-08-19

    DNA mismatch repair (MMR) is an evolutionarily-conserved process responsible for the repair of replication errors. In Escherichia coli, MMR is initiated by MutS and MutL, which activate MutH to incise transiently-hemimethylated GATC sites. MMR efficiency depends on the distribution of these GATC sites. To understand which molecular events determine repair efficiency, we quantitatively studied the effect of strand incision on unwinding and excision activity. The distance between mismatch and GATC site did not influence the strand incision rate, and an increase in the number of sites enhanced incision only to a minor extent. Two GATC sites were incised by the same activated MMR complex in a processive manner, with MutS, the closed form of MutL and MutH displaying different roles. Unwinding and strand excision were more efficient on a substrate with two nicks flanking the mismatch, as compared to substrates containing a single nick or two nicks on the same side of the mismatch. Introduction of multiple nicks by the human MutLα endonuclease also contributed to increased repair efficiency. Our data support a general model of prokaryotic and eukaryotic MMR in which, despite mechanistic differences, mismatch-activated complexes facilitate efficient repair by creating multiple daughter strand nicks.

  18. The replication initiator protein of plasmid pT181 has sequence-specific endonuclease and topoisomerase-like activities.

    PubMed Central

    Koepsel, R R; Murray, R W; Rosenblum, W D; Khan, S A

    1985-01-01

    Initiation of pT181 DNA replication specifically requires the plasmid-encoded RepC protein. Here we demonstrate that highly purified RepC protein has sequence-specific endonuclease and topoisomerase-like activities. A maximum sequence of 127 base pairs containing the pT181 origin of replication is required for nicking-closing by RepC protein. RepC introduces a single strand break within the pT181 origin. The nick site has been shown by DNA sequencing to lie between nucleotides 70 and 71 in the bottom strand of the DNA within the origin sequence. This nick site probably corresponds to the start site of pT181 replication. The results presented here suggest that, unlike most other plasmids, pT181 replicates by a rolling circle mechanism. Images PMID:2995991

  19. Maternal hurricane exposure and fetal distress risk.

    PubMed

    Zahran, Sammy; Snodgrass, Jeffrey G; Peek, Lori; Weiler, Stephan

    2010-10-01

    Logistic regression and spatial analytic techniques are used to model fetal distress risk as a function of maternal exposure to Hurricane Andrew. First, monthly time series compare the proportion of infants born distressed in hurricane affected and unaffected areas. Second, resident births are analyzed in Miami-Dade and Broward counties, before, during, and after Hurricane Andrew. Third, resident births are analyzed in all Florida locales with 100,000 or more persons, comparing exposed and unexposed gravid females. Fourth, resident births are analyzed along Hurricane Andrew's path from southern Florida to northeast Mississippi. Results show that fetal distress risk increases significantly with maternal exposure to Hurricane Andrew in second and third trimesters, adjusting for known risk factors. Distress risk also correlates with the destructive path of Hurricane Andrew, with higher incidences of fetal distress found in areas of highest exposure intensity. Hurricane exposed African-American mothers were more likely to birth distressed infants. The policy implications of in utero costs of natural disaster exposure are discussed.

  20. Officers and Council, 1998-1999

    NASA Astrophysics Data System (ADS)

    1999-10-01

    The 1998-1999 Council of the British Astronomical Association photographed on the steps of Burlington House, London, on 1999 May 26. Front row, left to right: Bob Marriott, Hazel McGee, Martin Mobberley, Nick James, Jonathan Shanklin, Ron Johnson; centre: Richard Miles, Gordon Taylor, Jacqueline Mitton, David Tucker, John Mason, Pat Barber, David Reid, Peter Hudson; back: Laurence Anslow, Guy Hurst, Lionel Mayling, Nick Hewitt, Owen Brazell, Tony Kinder, Mark Armstrong, Maurice Gavin. Photo by Hazel McGee and Linda Newton.