A refractory metamaterial absorber for ultra-broadband, omnidirectional and polarization-independent absorption in the UV-NIR spectrum.

PubMed

Huang, Yijia; Liu, Ling; Pu, Mingbo; Li, Xiong; Ma, Xiaoliang; Luo, Xiangang

2018-05-03

In this paper, efficient ultra-broadband absorption from ultraviolet (UV) to near infrared (NIR) is achieved using a metamaterial perfect absorber (MPA) with refractory constituents. Both simulated and experimental results indicate that this proposed MPA exhibits an average absorption over 95% at wavelengths ranging from 200 nm to 900 nm. Besides, owing to the ultrathin thickness and symmetrical topology of this device, it exhibits great angular tolerance up to 60° independent of the incident polarizations. Excellent thermal stability is also demonstrated at high operation temperatures. The physical origin of the ultra-broadband characteristics is mainly based on diffraction/interference engineering at short wavelengths and the anti-reflection effect at long wavelengths. We believe that such a device may find potential applications ranging from photodetection and photothermal energy conversion to ultraviolet protection and thermophotovoltaics.

Fluorescence Imaging In Vivo at Wavelengths beyond 1500 nm.

PubMed

Diao, Shuo; Blackburn, Jeffrey L; Hong, Guosong; Antaris, Alexander L; Chang, Junlei; Wu, Justin Z; Zhang, Bo; Cheng, Kai; Kuo, Calvin J; Dai, Hongjie

2015-12-01

Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly owing to the reduced scattering of photons traversing through biological tissues. Herein, semiconducting single-walled carbon nanotubes with large diameters were used for in vivo fluorescence imaging in the long-wavelength NIR region (1500-1700 nm, NIR-IIb). With this imaging agent, 3-4 μm wide capillary blood vessels at a depth of about 3 mm could be resolved. Meanwhile, the blood-flow speeds in multiple individual vessels could be mapped simultaneously. Furthermore, NIR-IIb tumor imaging of a live mouse was explored. NIR-IIb imaging can be generalized to a wide range of fluorophores emitting at up to 1700 nm for high-performance in vivo optical imaging. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Evaluation of factors in development of Vis/NIR spectroscopy models for discriminating PSE, DFD and normal broiler breast meat

USDA-ARS?s Scientific Manuscript database

1. To evaluate the performance of visible and near-infrared (Vis/NIR) spectroscopic models for discriminating true pale, soft and exudative (PSE), normal and dark, firm and dry (DFD) broiler breast meat in different conditions of preprocessing methods, spectral ranges, characteristic wavelength sele...

Near-infrared dental imaging using scanning fiber endoscope

NASA Astrophysics Data System (ADS)

Zhou, Yaxuan; Lee, Robert; Sadr, Alireza; Seibel, Eric J.

2018-02-01

Near-infrared (NIR) wavelength range of 1300-1500nm has the potential to outperform or augment other dental imaging modalities such as fluorescence imaging, owing to its lower scattering coefficient in enamel and trans- parency on stains and non-cariogenic plaque. However, cameras in this wavelength range are bulky and expensive, which lead to difficulties for in-vivo use and commercialization. Thus, we have proposed a new imaging device combining the scanning fiber endoscopy (SFE) and NIR imaging technology. The NIR SFE system has the advantage of miniature size (1.6 mm), flexible shaft, video frame rate (7Hz) and expandable wide field-of-view (60 degrees). Eleven extracted human teeth with or without occlusal caries were scanned by micro-computed X-ray tomography (micro-CT) to obtain 3D micro-CT images, which serve as the standard for comparison. NIR images in reflection mode were then taken on all the occlusal surfaces, using 1310nm super luminescent diode and 1460nm laser diode respectively. Qualitative comparison was performed between near-infrared im- ages and micro-CT images. Enamel demineralization in NIR appeared as areas of increased reflectivity, and distinguished from non-carious staining at the base of occlusal fissures or developmental defects on cusps. This preliminary work presented proof for practicability of combining NIR imaging technology with SFE for reliable and noninvasive dental imaging with miniaturization and low cost.

Prediction of quality attributes of chicken breast fillets by using Vis/NIR spectroscopy combined with factor analysis method

USDA-ARS?s Scientific Manuscript database

Visible/near-infrared (Vis/NIR) spectroscopy with wavelength range between 400 and 2500 nm combined with factor analysis method was tested to predict quality attributes of chicken breast fillets. Quality attributes, including color (L*, a*, b*), pH, and drip loss were analyzed using factor analysis ...

Individually Dispersed Gold Nanoshell-Bearing Cellulose Nanocrystals with Tailorable Plasmon Resonance.

PubMed

Semenikhin, Nikolay S; Kadasala, Naveen Reddy; Moon, Robert J; Perry, Joseph W; Sandhage, Kenneth H

2018-04-17

Cellulose nanocrystals (CNCs) can be attractive templates for the generation of functional inorganic/organic nanoparticles, given their fine sizes, aspect ratios, and sustainable worldwide availability in abundant quantities. Here, we present for the first time a scalable, surfactant-free, tailorable wet chemical process for converting commercially available CNCs into individual aspected gold nanoshell-bearing particles with tunable surface plasmon resonance bands. Using a rational cellulose functionalization approach, stable suspensions of positively charged CNCs have been generated. Continuous, conductive, nanocrystalline gold coatings were then applied to the individual, electrostatically stabilized CNCs via decoration with 1-3 nm diameter gold particles followed by electroless gold deposition. Optical analyses indicated that these core-shell nanoparticles exhibited two surface plasmon absorbance bands, with one located in the visible range (near 550 nm) and the other at near infrared (NIR) wavelengths. The NIR band possessed a peak maximum wavelength that could be tuned over a wide range (1000-1300 nm) by adjusting the gold coating thickness. The bandwidth and wavelength of the peak maximum of the NIR band were also sensitive to the particle size distribution and could be further refined by fractionation using viscosity gradient centrifugation.

Sensitivity Differences in Fish Offer Near-Infrared Vision as an Adaptable Evolutionary Trait

PubMed Central

Shcherbakov, Denis; Knörzer, Alexandra; Espenhahn, Svenja; Hilbig, Reinhard; Haas, Ulrich; Blum, Martin

2013-01-01

Near-infrared (NIR) light constitutes an integrated part of solar radiation. The principal ability to sense NIR under laboratory conditions has previously been demonstrated in fish. The availability of NIR in aquatic habitats, and thus its potential use as a cue for distinct behaviors such as orientation and detection of prey, however, depends on physical and environmental parameters. In clear water, blue and green light represents the dominating part of the illumination. In turbid waters, in contrast, the relative content of red and NIR radiation is enhanced, due to increased scattering and absorption of short and middle range wavelengths by suspended particles and dissolved colored materials. We have studied NIR detection thresholds using a phototactic swimming assay in five fish species, which are exposed to different NIR conditions in their natural habitats. Nile and Mozambique tilapia, which inhabit waters with increased turbidity, displayed the highest spectral sensitivity, with thresholds at wavelengths above 930 nm. Zebrafish, guppy and green swordtail, which prefer clearer waters, revealed significantly lower thresholds of spectral sensitivity with 825–845 nm for green swordtail and 845–910 nm for zebrafish and guppy. The present study revealed a clear correlation between NIR sensation thresholds and availability of NIR in the natural habitats, suggesting that NIR vision, as an integral part of the whole spectrum of visual abilities, can serve as an evolutionarily adaptable trait in fish. PMID:23691215

Searching for Water Earths in the Near-infrared

NASA Astrophysics Data System (ADS)

Zugger, M. E.; Kasting, J. F.; Williams, D. M.; Kane, T. J.; Philbrick, C. R.

2011-09-01

Over 500 extrasolar planets (exoplanets) have now been discovered, but only a handful are small enough that they might be rocky terrestrial planets like Venus, Earth, and Mars. Recently, it has been proposed that observations of variability in scattered light (both polarized and total flux) from such terrestrial-sized exoplanets could be used to determine if they possess large surface oceans, an important indicator of potential habitability. Observing such oceans at visible wavelengths would be difficult, however, in part because of obscuration by atmospheric scattering. Here, we investigate whether observations performed in the near-infrared (NIR), where Rayleigh scattering is reduced, could improve the detectability of exoplanet oceans. We model two wavebands of the NIR which are "window regions" for an Earth-like atmosphere: 1.55-1.75 μm and 2.1-2.3 μm. Our model confirms that obscuration in these bands from Rayleigh scattering is very low, but aerosols are generally the limiting factor throughout the wavelength range for Earth-like atmospheres. As a result, observations at NIR wavelengths are significantly better at detecting oceans than those at visible wavelengths only when aerosols are very thin by Earth standards. Clouds further dilute the ocean reflection signature. Hence, other techniques, e.g., time-resolved color photometry, may be more effective in the search for liquid water on exoplanet surfaces. Observing an exo-Earth at NIR wavelengths does open the possibility of detecting water vapor or other absorbers in the atmosphere, by comparing scattered light in window regions to that in absorption bands.

Determination of UV-visible-NIR absorption coefficient of graphite bulk using direct and indirect methods

NASA Astrophysics Data System (ADS)

Smausz, T.; Kondász, B.; Gera, T.; Ajtai, T.; Utry, N.; Pintér, M.; Kiss-Albert, G.; Budai, J.; Bozóki, Z.; Szabó, G.; Hopp, B.

2017-10-01

Absorption coefficient of graphite bulk pressed from 1 to 5 μm-sized crystalline grains was measured in UV-Vis-NIR range with three different methods: (i) determination of pulsed laser ablation rate as the function of laser fluence for different wavelengths (248, 337, 532, and 1064 nm, respectively); (ii) production of aerosol particles by UV laser ablation of the bulk graphite in inert atmosphere and determination of the mass-specific absorption coefficient with a four-wavelength (266, 355, 532, and 1064 nm, respectively) photoacoustic spectrometer, and (iii) spectroscopic ellipsometry in 250-1000 nm range. Taking into account the wide range of the absorption coefficients of different carbon structures, an overall relatively good agreement was observed for the three methods. The ellipsometric results fit well with the ablation rate measurement, and the data obtained with photoacoustic method are also similar in the UV and NIR region; however, the values were somewhat higher in visible and near-UV range. Taking into account the limitations of the methods, they can be promising candidates for the determination of absorption coefficient when the samples are strongly scattering and there is no possibility to perform transmissivity measurements.

Near-infrared imaging of demineralization under sealants

NASA Astrophysics Data System (ADS)

Tom, Henry; Simon, Jacob C.; Chan, Kenneth H.; Darling, Cynthia L.; Fried, Daniel

2014-07-01

Previous studies have shown that near-infrared (NIR) reflectance and transillumination imaging can be used to acquire high contrast images of early caries lesions and composite restorative materials. The aim of the study was to determine the optimum NIR wavelengths for imaging demineralized areas under dental sealants. Fifteen natural human premolars and molars with occlusal lesions were used in this in vitro study. Images before and after application of sealants were acquired using NIR reflectance and NIR transillumination at wavelengths of 1300, 1460, and 1500 to 1700 nm. Images were also acquired using polarization sensitive optical coherence tomography (OCT) for comparison. The highest contrast for NIR reflectance was at 1460 nm and 1500 to 1700 nm. These NIR wavelengths are coincident with higher water absorption. The clear Delton sealant investigated was not visible in either copolarization or cross-polarization OCT images. The wavelength region between 1500 and 1700 nm yielded the highest contrast of lesions under sealants for NIR reflectance measurements.

Near-infrared imaging of demineralization under sealants.

PubMed

Tom, Henry; Simon, Jacob C; Chan, Kenneth H; Darling, Cynthia L; Fried, Daniel

2014-01-01

Previous studies have shown that near-infrared (NIR) reflectance and transillumination imaging can be used to acquire high contrast images of early caries lesions and composite restorative materials. The aim of the study was to determine the optimum NIR wavelengths for imaging demineralized areas under dental sealants. Fifteen natural human premolars and molars with occlusal lesions were used in this in vitro study. Images before and after application of sealants were acquired using NIR reflectance and NIR transillumination at wavelengths of 1300, 1460, and 1500 to 1700 nm. Images were also acquired using polarization sensitive optical coherence tomography (OCT) for comparison. The highest contrast for NIR reflectance was at 1460 nm and 1500 to 1700 nm. These NIR wavelengths are coincident with higher water absorption. The clear Delton sealant investigated was not visible in either copolarization or cross-polarization OCT images. The wavelength region between 1500 and 1700 nm yielded the highest contrast of lesions under sealants for NIR reflectance measurements.

Photoacoustic imaging at 1064nm wavelength with exogenous contrast agents

NASA Astrophysics Data System (ADS)

Upputuri, Paul Kumar; Jiang, Yuyan; Pu, Kanyi; Pramanik, Manojit

2018-02-01

Photoacoustic (PA) imaging is a promising imaging modality for both preclinical research and clinical practices. Laser wavelengths in the first near infrared window (NIR-I, 650-950 nm) have been widely used for photoacoustic imaging. As compared with NIR-I window, scattering of photons by biological tissues is largely reduced in the second NIR (NIR-II) window, leading to enhanced imaging fidelity. However, the lack of biocompatible NIR-II absorbing exogenous agents prevented the use of this window for in vivo imaging. In recent years, few studies have been reported on photoacoustic imaging in NIR-II window using exogenous contrast agents. In this work, we discuss the recent work on PA imaging using 1064 nm wavelength, the fundamental of Nd:YAG laser, as an excitation wavelength. The PA imaging at 1064 nm is advantageous because of the low and homogeneous signal from tissue background, enabling high contrast in PA imaging when NIR-II absorbing contrast agents are employed.

Measurement of refractive index of hemoglobin in the visible/NIR spectral range

NASA Astrophysics Data System (ADS)

Lazareva, Ekaterina N.; Tuchin, Valery V.

2018-03-01

This study is focused on the measurements of the refractive index of hemoglobin solutions in the visible/near-infrared (NIR) spectral range at room temperature for characteristic laser wavelengths: 480, 486, 546, 589, 644, 656, 680, 930, 1100, 1300, and 1550 nm. Measurements were performed using the multiwavelength Abbe refractometer. Aqua hemoglobin solutions of different concentrations obtained from human whole blood were investigated. The specific increment of refractive index on hemoglobin concentration and the Sellmeier coefficients were calculated.

Multiparametric Flow Cytometry Using Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes

PubMed Central

Telford, William G.; Shcherbakova, Daria M.; Buschke, David; Hawley, Teresa S.; Verkhusha, Vladislav V.

2015-01-01

Engineering of fluorescent proteins (FPs) has followed a trend of achieving longer fluorescence wavelengths, with the ultimate goal of producing proteins with both excitation and emission in the near-infrared (NIR) region of the spectrum. Flow cytometers are now almost universally equipped with red lasers, and can now be equipped with NIR lasers as well. Most red-shifted FPs of the GFP-like family are maximally excited by orange lasers (590 to 610 nm) not commonly found on cytometers. This has changed with the development of the iRFP series of NIR FPs from the protein family of bacterial phytochromes. The shortest wavelength variants of this series, iRFP670 and iRFP682 showed maximal excitation with visible red lasers. The longer wavelength variants iRFP702, iRFP713 and iRFP720 could be optimally excited by NIR lasers ranging from 685 to 730 nm. Pairs of iRFPs could be detected simultaneously by using red and NIR lasers. Moreover, a novel spectral cytometry technique, which relies on spectral deconvolution rather than optical filters, allowed spectra of all five iRFPs to be analyzed simultaneously with no spectral overlap. Together, the combination of iRFPs with the advanced flow cytometry will allow to first image tissues expressing iRFPs deep in live animals and then quantify individual cell intensities and sort out the distinct primary cell subpopulations ex vivo. PMID:25811854

Multiparametric flow cytometry using near-infrared fluorescent proteins engineered from bacterial phytochromes.

PubMed

Telford, William G; Shcherbakova, Daria M; Buschke, David; Hawley, Teresa S; Verkhusha, Vladislav V

2015-01-01

Engineering of fluorescent proteins (FPs) has followed a trend of achieving longer fluorescence wavelengths, with the ultimate goal of producing proteins with both excitation and emission in the near-infrared (NIR) region of the spectrum. Flow cytometers are now almost universally equipped with red lasers, and can now be equipped with NIR lasers as well. Most red-shifted FPs of the GFP-like family are maximally excited by orange lasers (590 to 610 nm) not commonly found on cytometers. This has changed with the development of the iRFP series of NIR FPs from the protein family of bacterial phytochromes. The shortest wavelength variants of this series, iRFP670 and iRFP682 showed maximal excitation with visible red lasers. The longer wavelength variants iRFP702, iRFP713 and iRFP720 could be optimally excited by NIR lasers ranging from 685 to 730 nm. Pairs of iRFPs could be detected simultaneously by using red and NIR lasers. Moreover, a novel spectral cytometry technique, which relies on spectral deconvolution rather than optical filters, allowed spectra of all five iRFPs to be analyzed simultaneously with no spectral overlap. Together, the combination of iRFPs with the advanced flow cytometry will allow to first image tissues expressing iRFPs deep in live animals and then quantify individual cell intensities and sort out the distinct primary cell subpopulations ex vivo.

Near-Infrared Spectroscopy Using a Supercontinuum Laser: Application to Long Wavelength Transmission Spectra of Barley Endosperm and Oil.

PubMed

Ringsted, Tine; Dupont, Sune; Ramsay, Jacob; Jespersen, Birthe Møller; Sørensen, Klavs Martin; Keiding, Søren Rud; Engelsen, Søren Balling

2016-07-01

The supercontinuum laser is a new type of light source, which combines the collimation and intensity of a laser with the broad spectral region of a lamp. Using such a source therefore makes it possible to focus the light onto small sample areas without losing intensity and thus facilitate either rapid or high-intensity measurements. Single seed transmission analysis in the long wavelength (LW) near-infrared (NIR) region is one area that might benefit from a brighter light source such as the supercontinuum laser. This study is aimed at building an experimental spectrometer consisting of a supercontinuum laser source and a dispersive monochromator in order to investigate its capability to measure the barley endosperm using transmission experiments in the LW NIR region. So far, barley and wheat seeds have only been studied using NIR transmission in the short wavelength region up to 1100 nm. However, the region in the range of 2260-2380 nm has previously shown to be particularly useful in differentiating barley phenotypes using NIR spectroscopy in reflectance mode. In the present study, 350 seeds (consisting of 70 seeds from each of five barley genotypes) in 1 mm slices were measured by NIR transmission in the range of 2235-2381 nm and oils from the same five barley genotypes were measured in a cuvette with a 1 mm path length in the range of 2003-2497 nm. The spectra of the barley seeds could be classified according to genotypes by principal component analysis; and spectral covariances with reference analysis of moisture, β-glucan, starch, protein and lipid were established. The spectral variations of the barley oils were compared to the fatty acid compositions as measured using gas chromotography-mass spectrometry (GC-MS). © The Author(s) 2016.

Simultaneous generation of sub-5-femtosecond 400  nm and 800  nm pulses for attosecond extreme ultraviolet pump-probe spectroscopy.

PubMed

Chang, Hung-Tzu; Zürch, Michael; Kraus, Peter M; Borja, Lauren J; Neumark, Daniel M; Leone, Stephen R

2016-11-15

Few-cycle laser pulses with wavelengths centered at 400 nm and 800 nm are simultaneously obtained through wavelength separation of ultrashort, spectrally broadened Vis-NIR laser pulses spanning 350-1100 nm wavelengths. The 400 nm and 800 nm pulses are separately compressed, yielding pulses with 4.4 fs and 3.8 fs duration, respectively. The pulse energy exceeds 5 μJ for the 400 nm pulses and 750 μJ for the 800 nm pulses. Intense 400 nm few-cycle pulses have a broad range of applications in nonlinear optical spectroscopy, which include the study of photochemical dynamics, semiconductors, and photovoltaic materials on few-femtosecond to attosecond time scales. The ultrashort 400 nm few-cycle pulses generated here not only extend the spectral range of the optical pulse for NIR-XUV attosecond pump-probe spectroscopy but also pave the way for two-color, three-pulse, multidimensional optical-XUV spectroscopy experiments.

Energy-Looping Nanoparticles: Harnessing Excited-State Absorption for Deep-Tissue Imaging.

PubMed

Levy, Elizabeth S; Tajon, Cheryl A; Bischof, Thomas S; Iafrati, Jillian; Fernandez-Bravo, Angel; Garfield, David J; Chamanzar, Maysamreza; Maharbiz, Michel M; Sohal, Vikaas S; Schuck, P James; Cohen, Bruce E; Chan, Emory M

2016-09-27

Near infrared (NIR) microscopy enables noninvasive imaging in tissue, particularly in the NIR-II spectral range (1000-1400 nm) where attenuation due to tissue scattering and absorption is minimized. Lanthanide-doped upconverting nanocrystals are promising deep-tissue imaging probes due to their photostable emission in the visible and NIR, but these materials are not efficiently excited at NIR-II wavelengths due to the dearth of lanthanide ground-state absorption transitions in this window. Here, we develop a class of lanthanide-doped imaging probes that harness an energy-looping mechanism that facilitates excitation at NIR-II wavelengths, such as 1064 nm, that are resonant with excited-state absorption transitions but not ground-state absorption. Using computational methods and combinatorial screening, we have identified Tm(3+)-doped NaYF4 nanoparticles as efficient looping systems that emit at 800 nm under continuous-wave excitation at 1064 nm. Using this benign excitation with standard confocal microscopy, energy-looping nanoparticles (ELNPs) are imaged in cultured mammalian cells and through brain tissue without autofluorescence. The 1 mm imaging depths and 2 μm feature sizes are comparable to those demonstrated by state-of-the-art multiphoton techniques, illustrating that ELNPs are a promising class of NIR probes for high-fidelity visualization in cells and tissue.

Histological validation of near-infrared reflectance multispectral imaging technique for caries detection and quantification

NASA Astrophysics Data System (ADS)

Salsone, Silvia; Taylor, Andrew; Gomez, Juliana; Pretty, Iain; Ellwood, Roger; Dickinson, Mark; Lombardo, Giuseppe; Zakian, Christian

2012-07-01

Near infrared (NIR) multispectral imaging is a novel noninvasive technique that maps and quantifies dental caries. The technique has the ability to reduce the confounding effect of stain present on teeth. The aim of this study was to develop and validate a quantitative NIR multispectral imaging system for caries detection and assessment against a histological reference standard. The proposed technique is based on spectral imaging at specific wavelengths in the range from 1000 to 1700 nm. A total of 112 extracted teeth (molars and premolars) were used and images of occlusal surfaces at different wavelengths were acquired. Three spectral reflectance images were combined to generate a quantitative lesion map of the tooth. The maximum value of the map at the corresponding histological section was used as the NIR caries score. The NIR caries score significantly correlated with the histological reference standard (Spearman's Coefficient=0.774, p<0.01). Caries detection sensitivities and specificities of 72% and 91% for sound areas, 36% and 79% for lesions on the enamel, and 82% and 69% for lesions in dentin were found. These results suggest that NIR spectral imaging is a novel and promising method for the detection, quantification, and mapping of dental caries.

Histological validation of near-infrared reflectance multispectral imaging technique for caries detection and quantification.

PubMed

Salsone, Silvia; Taylor, Andrew; Gomez, Juliana; Pretty, Iain; Ellwood, Roger; Dickinson, Mark; Lombardo, Giuseppe; Zakian, Christian

2012-07-01

Near infrared (NIR) multispectral imaging is a novel noninvasive technique that maps and quantifies dental caries. The technique has the ability to reduce the confounding effect of stain present on teeth. The aim of this study was to develop and validate a quantitative NIR multispectral imaging system for caries detection and assessment against a histological reference standard. The proposed technique is based on spectral imaging at specific wavelengths in the range from 1000 to 1700 nm. A total of 112 extracted teeth (molars and premolars) were used and images of occlusal surfaces at different wavelengths were acquired. Three spectral reflectance images were combined to generate a quantitative lesion map of the tooth. The maximum value of the map at the corresponding histological section was used as the NIR caries score. The NIR caries score significantly correlated with the histological reference standard (Spearman's Coefficient=0.774, p<0.01). Caries detection sensitivities and specificities of 72% and 91% for sound areas, 36% and 79% for lesions on the enamel, and 82% and 69% for lesions in dentin were found. These results suggest that NIR spectral imaging is a novel and promising method for the detection, quantification, and mapping of dental caries.

Terrain Classification Using Multi-Wavelength Lidar Data

DTIC Science & Technology

2015-09-01

Figure 9. Pseudo- NDVI of three layers within the vertical structure of the forest. (Top) First return from the LiDAR instrument, including the ground...in NDVI throughout the vertical canopy. ........................................................17 Figure 10. Optech Titan operating wavelengths...and Ranging LMS LiDAR Mapping Suite ML Maximum Likelihood NIR Near Infrared N-D VIS n-Dimensional Visualizer NDVI Normalized Difference

Measurement of refractive index of hemoglobin in the visible/NIR spectral range.

PubMed

Lazareva, Ekaterina N; Tuchin, Valery V

2018-03-01

This study is focused on the measurements of the refractive index of hemoglobin solutions in the visible/near-infrared (NIR) spectral range at room temperature for characteristic laser wavelengths: 480, 486, 546, 589, 644, 656, 680, 930, 1100, 1300, and 1550 nm. Measurements were performed using the multiwavelength Abbe refractometer. Aqua hemoglobin solutions of different concentrations obtained from human whole blood were investigated. The specific increment of refractive index on hemoglobin concentration and the Sellmeier coefficients were calculated. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Identification of informative bands in the short-wavelength NIR region for non-invasive blood glucose measurement.

PubMed

Uwadaira, Yasuhiro; Ikehata, Akifumi; Momose, Akiko; Miura, Masayo

2016-07-01

The "glucose-linked wavelength" in the short-wavelength near-infrared (NIR) region, in which the light intensity reflected from the hand palm exhibits a good correlation to the blood glucose value, was investigated. We performed 391 2-h carbohydrate tolerance tests (CTTs) using 34 participants and a glucose-linked wavelength was successfully observed in almost every CTT; however, this wavelength varied between CTTs even for the same person. The large resulting data set revealed the distribution of the informative wavelength. The blood glucose values were efficiently estimated by a simple linear regression with clinically acceptable accuracies. The result suggested the potential for constructing a personalized low-invasive blood glucose sensor using short-wavelength NIR spectroscopy.

Optical modeling toward optimizing monitoring of intestinal perfusion in trauma patients

NASA Astrophysics Data System (ADS)

Akl, Tony J.; Wilson, Mark A.; Ericson, M. N.; Coté, Gerard L.

2013-02-01

Trauma is the number one cause of death for people between the ages 1 and 44 years in the United States. In addition, according to the Centers of Disease Control and Prevention, injury results in over 31 million emergency department visits annually. Minimizing the resuscitation period in major abdominal injuries increases survival rates by correcting impaired tissue oxygen delivery. Optimization of resuscitation requires a monitoring method to determine sufficient tissue oxygenation. Oxygenation can be assessed by determining the adequacy of tissue perfusion. In this work, we present the design of a wireless perfusion and oxygenation sensor based on photoplethysmography. Through optical modeling, the benefit of using the visible wavelengths 470, 525 and 590nm (around the 525nm hemoglobin isobestic point) for intestinal perfusion monitoring is compared to the typical near infrared (NIR) wavelengths (805nm isobestic point) used in such sensors. Specifically, NIR wavelengths penetrate through the thin intestinal wall ( 4mm) leading to high background signals. However, these visible wavelengths have two times shorter penetration depth that the NIR wavelengths. Monte-Carlo simulations show that the transmittance of the three selected wavelengths is lower by 5 orders of magnitude depending on the perfusion state. Due to the high absorbance of hemoglobin in the visible range, the perfusion signal carried by diffusely reflected light is also enhanced by an order of magnitude while oxygenation signal levels are maintained. In addition, short source-detector separations proved to be beneficial for limiting the probing depth to the thickness of the intestinal wall.

Visible-NIR Spectroscopic Evidence for the Composition of Low-Albedo Altered Soils on Mars

NASA Astrophysics Data System (ADS)

Murchie, S.; Merenyi, E.; Singer, R.; Kirkland, L.

1996-03-01

Spectroscopic studies of altered Martian soils at visible and at NIR wavelengths have generally supported the canonical model of the surface layer as consisting mostly of 2 components, bright red hematite-containing dust and dark gray pyroxene-containing sand. However several of the studies have also provided tantalizing evidence for distinct 1 micrometer Fe absorptions in discrete areas, particularly dark red soils which are hypothesized to consist of duricrust. These distinct absorptions have been proposed to originate from one or more non-hematitic ferric phases. We have tested this hypothesis by merging high spatial resolution visible- and NIR-wavelength data to synthesize composite 0.44-3.14 1lm spectra for regions of western Arabia and Margaritifer Terra. The extended wavelength coverage allows more complete assessment of ferric, ferrous, and H2O absorptions in both wavelength ranges. The composite data show that, compared to nearby bright red soil in Arabia, dark red soil in Oxia has a lower albedo, a more negative continuum slope, and a stronger 3 micrometer H2O absorption . However Fe absorptions are closely similar in position and depth. These results suggest that at least some dark red soils may differ from "normal" dust and mafic sand more in texture than in Fe mineralogy, although there appears to be enrichment in a water-containing phase and/or a dark, spectrally neutral phase. In contrast, there is clear evidence for enrichment of a low-albedo ferric mineral in dark gray soils composing Sinus Meridiani. These have visible- and NIR-wavelength absorptions consistent with crystalline hematite with relatively little pyroxene, plus a very weak 3 micrometer H2O absorption. These properties suggest a Ethology richer in crystalline hematite and less hydrated than both dust and mafic-rich sand.

A Survey of Near-infrared Diffuse Interstellar Bands

NASA Astrophysics Data System (ADS)

Hamano, Satochi; Kobayashi, Naoto; Kawakita, Hideyo; Ikeda, Yuji; Kondo, Sohei; Sameshima, Hiroaki; Arai, Akira; Matsunaga, Noriyuki; Yasui, Chikako; Mizumoto, Misaki; Fukue, Kei; Izumi, Natsuko; Otsubo, Shogo; Takenada, Keiichi

2018-04-01

We propose a study of interstellar molecules with near-infrared (NIR) high-resolution spectroscopy as a science case for the 3.6-m Devasthal Optical Telescope (DOT). In particular, we present the results obtained on-going survey of diffuse interstellar bands (DIBs) in NIR with the newly developed NIR high-resolution spectrograph WINERED, which offers a high sensitivity in the wavelength range of 0.91-1.36 µm. Using the WINERED spectrograph attached to the 1.3-m Araki telescope in Japan, we obtained high-quality spectra of a number of early-type stars in various environments, such as diffuse interstellar clouds, dark clouds and star-forming regions, to investigate the properties of NIR DIBs and constrain their carriers. As a result, we successfully identified about 50 new NIR DIBs, where only five fairly strong DIBs had been identified previously. Also, some properties of DIBs in the NIR are discussed to constrain the carriers of DIBs.

Near-infrared supercontinuum laser beam source in the second and third near-infrared optical windows used to image more deeply through thick tissue as compared with images from a lamp source

NASA Astrophysics Data System (ADS)

Sordillo, Laura A.; Lindwasser, Lukas; Budansky, Yury; Leproux, Philippe; Alfano, Robert R.

2015-03-01

With the use of longer near-infrared (NIR) wavelengths, image quality can be increased due to less scattering (described by the inverse wavelength power dependence 1/λn where n≥1) and minimal absorption from water molecules. Longer NIR windows, known as the second (1100 nm to 1350 nm) and third (1600 to 1870 nm) NIR windows are utilized to penetrate more deeply into tissue media and produce high-quality images. An NIR supercontinuum (SC) laser light source, with wavelengths in the second and third NIR optical windows to image tissue provides ballistic imaging of tissue. The SC ballistic beam can penetrate depths of up to 10 mm through tissue.

Low efficiency upconversion nanoparticles for high-resolution coalignment of near-infrared and visible light paths on a light microscope

PubMed Central

Sundaramoorthy, Sriramkumar; Badaracco, Adrian Garcia; Hirsch, Sophia M.; Park, Jun Hong; Davies, Tim; Dumont, Julien; Shirasu-Hiza, Mimi; Kummel, Andrew C.; Canman, Julie C.

2017-01-01

The combination of near infrared (NIR) and visible wavelengths in light microscopy for biological studies is increasingly common. For example, many fields of biology are developing the use of NIR for optogenetics, in which an NIR laser induces a change in gene expression and/or protein function. One major technical barrier in working with both NIR and visible light on an optical microscope is obtaining their precise coalignment at the imaging plane position. Photon upconverting particles (UCPs) can bridge this gap as they are excited by NIR light but emit in the visible range via an anti-Stokes luminescence mechanism. Here, two different UCPs have been identified, high-efficiency micro540-UCPs and lower efficiency nano545-UCPs, that respond to NIR light and emit visible light with high photostability even at very high NIR power densities (>25,000 Suns). Both of these UCPs can be rapidly and reversibly excited by visible and NIR light and emit light at visible wavelengths detectable with standard emission settings used for Green Fluorescent Protein (GFP), a commonly used genetically-encoded fluorophore. However, the high efficiency micro540-UCPs were suboptimal for NIR and visible light coalignment, due to their larger size and spatial broadening from particle-to-particle energy transfer consistent with a long lived excited state and saturated power dependence. In contrast, the lower efficiency nano-UCPs were superior for precise coalignment of the NIR beam with the visible light path (~2 µm versus ~8 µm beam broadening respectively) consistent with limited particle-to-particle energy transfer, superlinear power dependence for emission, and much smaller particle size. Furthermore, the nano-UCPs were superior to a traditional two-camera method for NIR and visible light path alignment in an in vivo Infrared-Laser-Evoked Gene Operator (IR-LEGO) optogenetics assay in the budding yeast S. cerevisiae. In summary, nano-UCPs are powerful new tools for coaligning NIR and visible light paths on a light microscope. PMID:28221018

MULTIMODAL IMAGING OF DISEASE-ASSOCIATED PIGMENTARY CHANGES IN RETINITIS PIGMENTOSA.

PubMed

Schuerch, Kaspar; Marsiglia, Marcela; Lee, Winston; Tsang, Stephen H; Sparrow, Janet R

2016-12-01

Using multiple imaging modalities, we evaluated the changes in photoreceptor cells and retinal pigment epithelium (RPE) that are associated with bone spicule-shaped melanin pigmentation in retinitis pigmentosa. In a cohort of 60 patients with retinitis pigmentosa, short-wavelength autofluorescence, near-infrared autofluorescence (NIR-AF), NIR reflectance, spectral domain optical coherence tomography, and color fundus images were studied. Central AF rings were visible in both short-wavelength autofluorescence and NIR-AF images. Bone spicule pigmentation was nonreflective in NIR reflectance, hypoautofluorescent with short-wavelength autofluorescence and NIR-AF imaging, and presented as intraretinal hyperreflective foci in spectral domain optical coherence tomography images. In areas beyond the AF ring outer border, the photoreceptor ellipsoid zone band was absent in spectral domain optical coherence tomography and the visibility of choroidal vessels in short-wavelength autofluorescence, NIR-AF, and NIR reflectance images was indicative of reduced RPE pigmentation. Choroidal visibility was most pronounced in the zone approaching peripheral areas of bone spicule pigmentation; here RPE/Bruch membrane thinning became apparent in spectral domain optical coherence tomography. These findings are consistent with a process by which RPE cells vacate their monolayer and migrate into inner retina in response to photoreceptor cell degeneration. The remaining RPE spread undergo thinning and consequently become less pigmented. An explanation for the absence of NIR-AF melanin signal in relation to bone spicule pigmentation is not forthcoming.

A near-infrared fluorescent probe based on BODIPY derivative with high quantum yield for selective detection of exogenous and endogenous cysteine in biological samples.

PubMed

Li, Song-Jiao; Fu, Ya-Jun; Li, Chun-Yan; Li, Yong-Fei; Yi, Lan-Hua; Ou-Yang, Juan

2017-11-22

Cysteine (Cys) is involved in cellular growth and Cys deficiency is related with many diseases. So far, a number of fluorescent probes have been constructed for the detection of Cys successfully. However, the probes are difficult to discriminate Cys from Hcy and the emission wavelength of the probes is in ultraviolet or visible range. Herein, a NIR fluorescent probe named NIR-BODIPY-Ac is synthesized and used to detect Cys. The emission wavelength of the probe is at 708 nm that belongs to near-infrared (NIR) region by attaching indolium to BODIPY core, which is suitable for bioimaging in vivo. Moreover, the probe exhibits high fluorescence quantum yield (Φ = 0.51) after the addition of Cys and high sensitivity toward Cys with 81-fold fluorescence enhancement. The linear range of the probe for Cys covers from 0.2 to 30 μM with a detection limit of 0.05 μM. Furthermore, the probe shows high selectivity towards Cys owing to the fact that there is more fast reaction rate between the probe and Cys than that of Hcy. In particular, the NIR fluorescent probe is applied for the detection of exogenous and endogenous Cys in biological samples such as cell, tissue and mouse with satisfactory results. Copyright © 2017 Elsevier B.V. All rights reserved.

Optical modeling toward optimizing monitoring of intestinal perfusion in trauma patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Akl, Tony; Wilson, Mark A.; Ericson, Milton Nance

2013-01-01

Trauma is the number one cause of death for people between the ages 1 and 44 years in the United States. In addition, according to the Centers of Disease Control and Prevention, injury results in over 31 million emergency department visits annually. Minimizing the resuscitation period in major abdominal injuries increases survival rates by correcting impaired tissue oxygen delivery. Optimization of resuscitation requires a monitoring method to determine sufficient tissue oxygenation. Oxygenation can be assessed by determining the adequacy of tissue perfusion. In this work, we present the design of a wireless perfusion and oxygenation sensor based on photoplethysmography. Throughmore » optical modeling, the benefit of using the visible wavelengths 470, 525 and 590nm (around the 525nm hemoglobin isobestic point) for intestinal perfusion monitoring is compared to the typical near infrared (NIR) wavelengths (805nm isobestic point) used in such sensors. Specifically, NIR wavelengths penetrate through the thin intestinal wall (~4mm) leading to high background signals. However, these visible wavelengths have two times shorter penetration depth that the NIR wavelengths. Monte-Carlo simulations show that the transmittance of the three selected wavelengths is lower by 5 orders of magnitude depending on the perfusion state. Due to the high absorbance of hemoglobin in the visible range, the perfusion signal carried by diffusely reflected light is also enhanced by an order of magnitude while oxygenation signal levels are maintained. In addition, short source-detector separations proved to be beneficial for limiting the probing depth to the thickness of the intestinal wall.« less

Fast and simultaneous prediction of animal feed nutritive values using near infrared reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Samadi; Wajizah, S.; Munawar, A. A.

2018-02-01

Feed plays an important factor in animal production. The purpose of this study is to apply NIRS method in determining feed values. NIRS spectra data were acquired for feed samples in wavelength range of 1000 - 2500 nm with 32 scans and 0.2 nm wavelength. Spectral data were corrected by de-trending (DT) and standard normal variate (SNV) methods. Prediction of in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) were established as model by using principal component regression (PCR) and validated using leave one out cross validation (LOOCV). Prediction performance was quantified using coefficient correlation (r) and residual predictive deviation (RPD) index. The results showed that IVDMD and IVOMD can be predicted by using SNV spectra data with r and RPD index: 0.93 and 2.78 for IVDMD ; 0.90 and 2.35 for IVOMD respectively. In conclusion, NIRS technique appears feasible to predict animal feed nutritive values.

Development of near infrared spectrometer for gem materials study

NASA Astrophysics Data System (ADS)

Jindata, W.; Meesiri, W.; Wongkokua, W.

2015-07-01

Most of gem materials can be characterized by infrared absorption spectroscopy. Normally, mid infrared absorption technique has been applied for investigating fundamental vibrational modes. However, for some gem materials, such as tourmaline, NIR is a better choice due to differentiation. Most commercial NIR spectrometers employ complicated dispersive grating or Fourier transform techniques. In this work, we developed a filter type NIR spectrometer with the availability of high efficiency and low-cost narrow bandpass NIR interference filters to be taught in a physics laboratory. The instrument was designed for transmission-mode configuration. A 50W halogen lamp was used as NIR source. There were fourteen NIR filters mounted on a rotatory wheel for wavelength selection ranging from 1000-1650 nm with steps of 50 nm. A 1.0 mm diameter of InGaAs photodiode was used as the detector for the spectrometer. Hence, transparent gem materials can be used as samples for experiment. Student can learn vibrational absorption spectroscopy as well as Beer-Lambert law from the development of this instrument.

[Determination of soluble solids content in Nanfeng Mandarin by Vis/NIR spectroscopy and UVE-ICA-LS-SVM].

PubMed

Sun, Tong; Xu, Wen-Li; Hu, Tian; Liu, Mu-Hua

2013-12-01

The objective of the present research was to assess soluble solids content (SSC) of Nanfeng mandarin by visible/near infrared (Vis/NIR) spectroscopy combined with new variable selection method, simplify prediction model and improve the performance of prediction model for SSC of Nanfeng mandarin. A total of 300 Nanfeng mandarin samples were used, the numbers of Nanfeng mandarin samples in calibration, validation and prediction sets were 150, 75 and 75, respectively. Vis/NIR spectra of Nanfeng mandarin samples were acquired by a QualitySpec spectrometer in the wavelength range of 350-1000 nm. Uninformative variables elimination (UVE) was used to eliminate wavelength variables that had few information of SSC, then independent component analysis (ICA) was used to extract independent components (ICs) from spectra that eliminated uninformative wavelength variables. At last, least squares support vector machine (LS-SVM) was used to develop calibration models for SSC of Nanfeng mandarin using extracted ICs, and 75 prediction samples that had not been used for model development were used to evaluate the performance of SSC model of Nanfeng mandarin. The results indicate t hat Vis/NIR spectroscopy combinedwith UVE-ICA-LS-SVM is suitable for assessing SSC o f Nanfeng mandarin, and t he precision o f prediction ishigh. UVE--ICA is an effective method to eliminate uninformative wavelength variables, extract important spectral information, simplify prediction model and improve the performance of prediction model. The SSC model developed by UVE-ICA-LS-SVM is superior to that developed by PLS, PCA-LS-SVM or ICA-LS-SVM, and the coefficient of determination and root mean square error in calibration, validation and prediction sets were 0.978, 0.230%, 0.965, 0.301% and 0.967, 0.292%, respectively.

[Application of characteristic NIR variables selection in portable detection of soluble solids content of apple by near infrared spectroscopy].

PubMed

Fan, Shu-Xiang; Huang, Wen-Qian; Li, Jiang-Bo; Guo, Zhi-Ming; Zhaq, Chun-Jiang

2014-10-01

In order to detect the soluble solids content(SSC)of apple conveniently and rapidly, a ring fiber probe and a portable spectrometer were applied to obtain the spectroscopy of apple. Different wavelength variable selection methods, including unin- formative variable elimination (UVE), competitive adaptive reweighted sampling (CARS) and genetic algorithm (GA) were pro- posed to select effective wavelength variables of the NIR spectroscopy of the SSC in apple based on PLS. The back interval LS- SVM (BiLS-SVM) and GA were used to select effective wavelength variables based on LS-SVM. Selected wavelength variables and full wavelength range were set as input variables of PLS model and LS-SVM model, respectively. The results indicated that PLS model built using GA-CARS on 50 characteristic variables selected from full-spectrum which had 1512 wavelengths achieved the optimal performance. The correlation coefficient (Rp) and root mean square error of prediction (RMSEP) for prediction sets were 0.962, 0.403°Brix respectively for SSC. The proposed method of GA-CARS could effectively simplify the portable detection model of SSC in apple based on near infrared spectroscopy and enhance the predictive precision. The study can provide a reference for the development of portable apple soluble solids content spectrometer.

Quantitative interpretations of Visible-NIR reflectance spectra of blood.

PubMed

Serebrennikova, Yulia M; Smith, Jennifer M; Huffman, Debra E; Leparc, German F; García-Rubio, Luis H

2008-10-27

This paper illustrates the implementation of a new theoretical model for rapid quantitative analysis of the Vis-NIR diffuse reflectance spectra of blood cultures. This new model is based on the photon diffusion theory and Mie scattering theory that have been formulated to account for multiple scattering populations and absorptive components. This study stresses the significance of the thorough solution of the scattering and absorption problem in order to accurately resolve for optically relevant parameters of blood culture components. With advantages of being calibration-free and computationally fast, the new model has two basic requirements. First, wavelength-dependent refractive indices of the basic chemical constituents of blood culture components are needed. Second, multi-wavelength measurements or at least the measurements of characteristic wavelengths equal to the degrees of freedom, i.e. number of optically relevant parameters, of blood culture system are required. The blood culture analysis model was tested with a large number of diffuse reflectance spectra of blood culture samples characterized by an extensive range of the relevant parameters.

Active modulation of laser coded systems using near infrared video projection system based on digital micromirror device (DMD)

NASA Astrophysics Data System (ADS)

Khalifa, Aly A.; Aly, Hussein A.; El-Sherif, Ashraf F.

2016-02-01

Near infrared (NIR) dynamic scene projection systems are used to perform hardware in-the-loop (HWIL) testing of a unit under test operating in the NIR band. The common and complex requirement of a class of these units is a dynamic scene that is spatio-temporal variant. In this paper we apply and investigate active external modulation of NIR laser in different ranges of temporal frequencies. We use digital micromirror devices (DMDs) integrated as the core of a NIR projection system to generate these dynamic scenes. We deploy the spatial pattern to the DMD controller to simultaneously yield the required amplitude by pulse width modulation (PWM) of the mirror elements as well as the spatio-temporal pattern. Desired modulation and coding of high stable, high power visible (Red laser at 640 nm) and NIR (Diode laser at 976 nm) using the combination of different optical masks based on DMD were achieved. These spatial versatile active coding strategies for both low and high frequencies in the range of kHz for irradiance of different targets were generated by our system and recorded using VIS-NIR fast cameras. The temporally-modulated laser pulse traces were measured using array of fast response photodetectors. Finally using a high resolution spectrometer, we evaluated the NIR dynamic scene projection system response in terms of preserving the wavelength and band spread of the NIR source after projection.

All-inorganic colloidal upconversion quantum dots (Conference Presentation)

NASA Astrophysics Data System (ADS)

Oron, Dan; Teitelboim, Ayelet

2017-02-01

Upconversion (UC) is a nonlinear process in which two, or more, long wavelength photons are converted to a shorter wavelength photon. This process is based on sequential absorption of two or more photons, involving metastable, long lived intermediate energy states, thus is not restricted to ultrashort pulsed excitation. Hence, requirements for UC processes are long lived excited states, a ladder like arrangement of energy levels and a mechanism inhibiting cooling of the hot charge carrier. UC holds great promise for bioimaging, enabling to perform multiphoton imaging in scattering specimen at very low powers. Rare-earth-doped nanocrystals, the most commonly used ones for UC, typically require a minimal particle diameter of several tens of nanometers and have a limited action spectrum. Here, we present a novel luminescence upconversion nano-system based on colloidal semiconductor double quantum dots, consisting of a NIR-absorbing component and a visible emitting component separated by a tunneling barrier in a spherical onion-like geometry. These dual near-infrared and visible core/shell/shell PbSe/CdSe/CdS nanocrystals are shown to efficiently upconvert a broad range of NIR wavelengths up to 1.2 microns to visible emission at room temperature, covering a spectral range where there are practically no alternative upconversion systems. The particle diameter is less than ten nanometers, and the synthesis enables versatility and tunability of both the visible emission color and the NIR absorption edge. The physical mechanism for upconversion in this type of structures, as well as potential advances and extensions on this system will be discussed.

Setup for testing cameras for image guided surgery using a controlled NIR fluorescence mimicking light source and tissue phantom

NASA Astrophysics Data System (ADS)

Georgiou, Giota; Verdaasdonk, Rudolf M.; van der Veen, Albert; Klaessens, John H.

2017-02-01

In the development of new near-infrared (NIR) fluorescence dyes for image guided surgery, there is a need for new NIR sensitive camera systems that can easily be adjusted to specific wavelength ranges in contrast the present clinical systems that are only optimized for ICG. To test alternative camera systems, a setup was developed to mimic the fluorescence light in a tissue phantom to measure the sensitivity and resolution. Selected narrow band NIR LED's were used to illuminate a 6mm diameter circular diffuse plate to create uniform intensity controllable light spot (μW-mW) as target/source for NIR camera's. Layers of (artificial) tissue with controlled thickness could be placed on the spot to mimic a fluorescent `cancer' embedded in tissue. This setup was used to compare a range of NIR sensitive consumer's cameras for potential use in image guided surgery. The image of the spot obtained with the cameras was captured and analyzed using ImageJ software. Enhanced CCD night vision cameras were the most sensitive capable of showing intensities < 1 μW through 5 mm of tissue. However, there was no control over the automatic gain and hence noise level. NIR sensitive DSLR cameras proved relative less sensitive but could be fully manually controlled as to gain (ISO 25600) and exposure time and are therefore preferred for a clinical setting in combination with Wi-Fi remote control. The NIR fluorescence testing setup proved to be useful for camera testing and can be used for development and quality control of new NIR fluorescence guided surgery equipment.

Refractive index sensing in the visible/NIR spectrum using silicon nanopillar arrays.

PubMed

Visser, D; Choudhury, B Dev; Krasovska, I; Anand, S

2017-05-29

Si nanopillar (NP) arrays are investigated as refractive index sensors in the visible/NIR wavelength range, suitable for Si photodetector responsivity. The NP arrays are fabricated by nanoimprint lithography and dry etching, and coated with thin dielectric layers. The reflectivity peaks obtained by finite-difference time-domain (FDTD) simulations show a linear shift with coating layer thickness. At 730 nm wavelength, sensitivities of ~0.3 and ~0.9 nm/nm of SiO 2 and Si 3 N 4 , respectively, are obtained; and the optical thicknesses of the deposited surface coatings are determined by comparing the experimental and simulated data. The results show that NP arrays can be used for sensing surface bio-layers. The proposed method could be useful to determine the optical thickness of surface coatings, conformal and non-conformal, in NP-based optical devices.

Smart filters: from VIS/NIR to MW/LWIR protection

NASA Astrophysics Data System (ADS)

Donval, Ariela; Fisher, Tali; Lipman, Ofir; Oron, Moshe

2014-06-01

New development of imaging systems implies the use of multi band wavelength, VIS and IR, for imaging enhancement and more data presenting. Some of those systems, such as

A Multi-Wavelength IR Laser for Space Applications

NASA Technical Reports Server (NTRS)

Li, Steven X.; Yu, Anthony W.; Sun, Xiaoli; Fahey, Molly E.; Numata, Kenji; Krainak, Michael A.

2017-01-01

We present a laser technology development with space flight heritage to generate laser wavelengths in the near- to mid-infrared (NIR to MIR) for space lidar applications. Integrating an optical parametric crystal to the LOLA (Lunar Orbiter Laser Altimeter) laser transmitter design affords selective laser wavelengths from NIR to MIR that are not easily obtainable from traditional diode pumped solid-state lasers. By replacing the output coupler of the LOLA laser with a properly designed parametric crystal, we successfully demonstrated a monolithic intra-cavity optical parametric oscillator (iOPO) laser based on all high technology readiness level (TRL) subsystems and components. Several desired wavelengths have been generated including 2.1 microns, 2.7 microns and 3.4 microns. This laser can also be used in trace-gas remote sensing, as many molecules possess their unique vibrational transitions in NIR to MIR wavelength region, as well as in time-of-flight mass spectrometer where desorption of samples using MIR laser wavelengths have been successfully demonstrated.

A multi-wavelength IR laser for space applications

NASA Astrophysics Data System (ADS)

Li, Steven X.; Yu, Anthony W.; Sun, Xiaoli; Fahey, Molly E.; Numata, Kenji; Krainak, Michael A.

2017-05-01

We present a laser technology development with space flight heritage to generate laser wavelengths in the near- to midinfrared (NIR to MIR) for space lidar applications. Integrating an optical parametric crystal to the LOLA (Lunar Orbiter Laser Altimeter) laser transmitter design affords selective laser wavelengths from NIR to MIR that are not easily obtainable from traditional diode pumped solid-state lasers. By replacing the output coupler of the LOLA laser with a properly designed parametric crystal, we successfully demonstrated a monolithic intra-cavity optical parametric oscillator (iOPO) laser based on all high technology readiness level (TRL) subsystems and components. Several desired wavelengths have been generated including 2.1 µm, 2.7 μm and 3.4 μm. This laser can also be used in trace-gas remote sensing, as many molecules possess their unique vibrational transitions in NIR to MIR wavelength region, as well as in time-of-flight mass spectrometer where desorption of samples using MIR laser wavelengths have been successfully demonstrated

Near-Infrared Scintillation of Liquid Argon

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tilly, Elizabeth; Escobar, Carlos

2017-01-01

Liquid argon is well known to scintillate in the vacuum ultraviolet (VUV) range which is inherently difficult to detect. There has been recent evidence to suggest that it also emits near infrared (NIR) light. If this is the case, many large-scale time projection chambers and other similar detectors will be able to maximize light collection while minimizing cost. The goal of this project is to confirm and quantify this NIR emission. In order to accomplish this, an α-source was placed in a volume of highly purified liquid argon and observed using an infrared PMT with a filter excluding light withmore » wavelength <715 nm. Performing a simple counting experiment, there were indications of NIR scintillation. Further analysis is in progress.« less

A Brief Review of OPT101 Sensor Application in Near-Infrared Spectroscopy Instrumentation for Intensive Care Unit Clinics

PubMed Central

Li, Ting; Zhong, Fulin; Pan, Boan; Li, Zebin; Huang, Chong; Deng, Zishan

2017-01-01

The optoelectronic sensor OPT101 have merits in advanced optoelectronic response characteristics at wavelength range for medical near-infrared spectroscopy and small-size chip design with build-in trans-impedance amplifier. Our lab is devoted to developing a series of portable near-infrared spectroscopy (NIRS) devices embedded with OPT101 for applications in intensive care unit clinics, based on NIRS principle. Here we review the characteristics and advantages of OPT101 relative to clinical NIRS instrumentation, and the most recent achievements, including early-diagnosis and therapeutic effect evaluation of thrombus, noninvasive monitoring of patients' shock severity, and fatigue evaluation. The future prospect on OPT101 improvements in noninvasive clinical applications is also discussed. PMID:28757564

Correlations Among Near-Infrared and Short-Wavelength Autofluorescence and Spectral-Domain Optical Coherence Tomography in Recessive Stargardt Disease

PubMed Central

Duncker, Tobias; Marsiglia, Marcela; Lee, Winston; Zernant, Jana; Tsang, Stephen H.; Allikmets, Rando; Greenstein, Vivienne C.; Sparrow, Janet R.

2014-01-01

Purpose. Short-wavelength (SW) fundus autofluorescence (AF) is considered to originate from lipofuscin in retinal pigment epithelium (RPE) and near-infrared (NIR) AF from melanin. In patients with recessive Stargardt disease (STGD1), we correlated SW-AF and NIR-AF with structural information obtained by spectral-domain optical coherence tomography (SD-OCT). Methods. Twenty-four STGD1 patients (45 eyes; age 8 to 61 years) carrying confirmed disease-associated ABCA4 mutations were studied prospectively. Short-wavelength AF, NIR-AF, and SD-OCT images were acquired. Results. Five phenotypes were identified according to features of the central lesion and extent of fundus change. Central zones of reduced NIR-AF were typically larger than areas of diminished SW-AF and reduced NIR-AF usually approximated areas of ellipsoid zone (EZ) loss identified by SD-OCT (group 1; r, 0.93, P < 0.0001). In patients having a central lesion with overlapping parafoveal rings of increased NIR-AF and SW-AF (group 3), the extent of EZ loss was strongly correlated with the inner diameter of the NIR-AF ring (r, 0.89, P < 0.0001) and the eccentricity of the outer border of the NIR-AF ring was greater than that of the SW-AF ring. Conclusions. Lesion areas were more completely delineated in NIR-AF images than with SW-AF. In most cases, EZ loss was observed only at locations where NIR-AF was reduced or absent, indicating that RPE cell atrophy occurs in advance of photoreceptor cell degeneration. Because SW-AF was often increased within the central area of EZ disruption, degenerating photoreceptor cells may produce lipofuscin at accelerated levels. Consideration is given to mechanisms underlying hyper–NIR-AF in conjunction with increased SW-AF. PMID:25342616

Compensation of spectral artifacts in dual-modality intravascular optical coherence tomography and near-infrared spectroscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Fard, Ali M.; Gardecki, Joseph A.; Ughi, Giovanni J.; Hyun, Chulho; Tearney, Guillermo J.

2016-02-01

Intravascular optical coherence tomography (OCT) is a high-resolution catheter-based imaging method that provides three-dimensional microscopic images of coronary artery in vivo, facilitating coronary artery disease treatment decisions based on detailed morphology. Near-infrared spectroscopy (NIRS) has proven to be a powerful tool for identification of lipid-rich plaques inside the coronary walls. We have recently demonstrated a dual-modality intravascular imaging technology that integrates OCT and NIRS into one imaging catheter using a two-fiber arrangement and a custom-made dual-channel fiber rotary junction. It therefore enables simultaneous acquisition of microstructural and composition information at 100 frames/second for improved diagnosis of coronary lesions. The dual-modality OCT-NIRS system employs a single wavelength-swept light source for both OCT and NIRS modalities. It subsequently uses a high-speed photoreceiver to detect the NIRS spectrum in the time domain. Although use of one light source greatly simplifies the system configuration, such light source exhibits pulse-to-pulse wavelength and intensity variation due to mechanical scanning of the wavelength. This can be in particular problematic for NIRS modality and sacrifices the reliability of the acquired spectra. In order to address this challenge, here we developed a robust data acquisition and processing method that compensates for the spectral variations of the wavelength-swept light source. The proposed method extracts the properties of the light source, i.e., variation period and amplitude from a reference spectrum and subsequently calibrates the NIRS datasets. We have applied this method on datasets obtained from cadaver human coronary arteries using a polygon-scanning (1230-1350nm) OCT system, operating at 100,000 sweeps per second. The results suggest that our algorithm accurately and robustly compensates the spectral variations and visualizes the dual-modality OCT-NIRS images. These findings are therefore crucial for the practical application and clinical translation of dual-modality intravascular OCT-NIRS imaging when the same swept sources are used for both OCT and spectroscopy.

Near-infrared fluorescence imaging using organic dye nanoparticles.

PubMed

Yu, Jia; Zhang, Xiujuan; Hao, Xiaojun; Zhang, Xiaohong; Zhou, Mengjiao; Lee, Chun-Sing; Chen, Xianfeng

2014-03-01

Near-infrared (NIR) fluorescence imaging in the 700-1000 nm wavelength range has been very attractive for early detection of cancers. Conventional NIR dyes often suffer from limitation of low brightness due to self-quenching, insufficient photo- and bioenvironmental stability, and small Stokes shift. Herein, we present a strategy of using small-molecule organic dye nanoparticles (ONPs) to encapsulate NIR dyes to enable efficient fluorescence resonance energy transfer to obtain NIR probes with remarkably enhanced performance for in vitro and in vivo imaging. In our design, host ONPs are used as not only carriers to trap and stabilize NIR dyes, but also light-harvesting agent to transfer energy to NIR dyes to enhance their brightness. In comparison with pure NIR dyes, our organic dye nanoparticles possess almost 50-fold increased brightness, large Stokes shifts (∼250 nm) and dramatically enhanced photostability. With surface modification, these NIR-emissive organic nanoparticles have water-dispersity and size- and fluorescence- stability over pH values from 2 to 10 for almost 60 days. With these superior advantages, these NIR-emissive organic nanoparticles can be used for highly efficient folic-acid aided specific targeting in vivo and ex vivo cellular imaging. Finally, during in vivo imaging, the nanoparticles show negligible toxicity. Overall, the results clearly display a potential application of using the NIR-emissive organic nanoparticles for in vitro and in vivo imaging. Copyright © 2014 Elsevier Ltd. All rights reserved.

Relationships between NIR spectra and sensory attributes of Thai commercial fish sauces.

PubMed

Ritthiruangdej, Pitiporn; Suwonsichon, Thongchai

2007-07-01

Twenty Thai commercial fish sauces were characterized by sensory descriptive analysis and near-infrared (NIR) spectroscopy. The main objectives were i) to investigate the relationships between sensory attributes and NIR spectra of samples and ii) to characterize the sensory characteristics of fish sauces based on NIR data. A generic descriptive analysis with 12 trained panels was used to characterize the sensory attributes. These attributes consisted of 15 descriptors: brown color, 5 aromatics (sweet, caramelized, fermented, fishy, and musty), 4 tastes (sweet, salty, bitter, and umami), 3 aftertastes (sweet, salty and bitter) and 2 flavors (caramelized and fishy). The results showed that Thai fish sauce samples exhibited significant differences in all of sensory attribute values (p < 0.05). NIR transflectance spectra were obtained from 1100 to 2500 nm. Prior to investigation of the relationships between sensory attributes and NIR spectra, principal component analysis (PCA) was applied to reduce the dimensionality of the spectral data from 622 wavelengths to two uncorrelated components (NIR1 and NIR2) which explained 92 and 7% of the total variation, respectively. NIR1 was highly correlated with the wavelength regions of 1100 - 1544, 1774 - 2062, 2092 - 2308, and 2358 - 2440 nm, while NIR2 was highly correlated with the wavelength regions of 1742 - 1764, 2066 - 2088, and 2312 - 2354 nm. Subsequently, the relationships among these two components and all sensory attributes were also investigated by PCA. The results showed that the first three principal components (PCs) named as fishy flavor component (PC1), sweet component (PC2) and bitterness component (PC3), respectively, explained a total of 66.86% of the variation. NIR1 was mainly correlated to the sensory attributes of fishy aromatic, fishy flavor and sweet aftertaste on PC1. In addition, the PCA using only the factor loadings of NIR1 and NIR2 could be used to classify samples into three groups which showed high, medium and low degrees of fishy aromatic, fishy flavor and sweet aftertaste.

Monolithic photonic integration for visible and short near-infrared wavelengths: technologies and platforms for bio and life science applications

NASA Astrophysics Data System (ADS)

Porcel, Marco A. G.; Artundo, Iñigo; Domenech, J. David; Geuzebroek, Douwe; Sunarto, Rino; Hoofman, Romano

2018-04-01

This tutorial aims to provide a general overview on the state-of-the-art of photonic integrated circuits (PICs) in the visible and short near-infrared (NIR) wavelength ranges, mostly focusing in silicon nitride (SiN) substrates, and a guide to the necessary steps in the design toward the fabrication of such PICs. The focus is put on bio- and life sciences, given the adequacy and, thus, a large number of applications in this field.

Regional analysis of tertiary volcanic Calderas (western U.S.) using Landsat Thematic Mapper imagery

NASA Technical Reports Server (NTRS)

Spatz, David M.; Taranik, James V.

1989-01-01

The Landsat Thematic Mapper (TM) imagery of the Basin and Range province of southern Nevada was analyzed to identify and map volcanic rock assemblages at three Tertiary calderas. It was found that the longer-wavelength visible and the NIR TM Bands 3, 5, and 7 provide more effective lithologic discrimination than the shorter-wavelength bands, due partly to deeper penetration of the longer-wavelength bands, resulting in more lithologically driven radiances. Shorter-wavelength TM Bands 1 and 2 are affected more by surficial weathering products including desert varnish which may or may not provide an indirect link to lithologic identity. Guidelines for lithologic analysis of volcanic terrains using Landsat TM imagery are outlined.

Spectral signatures of photosynthesis. I. Review of Earth organisms.

PubMed

Kiang, Nancy Y; Siefert, Janet; Govindjee; Blankenship, Robert E

2007-02-01

Why do plants reflect in the green and have a "red edge" in the red, and should extrasolar photosynthesis be the same? We provide (1) a brief review of how photosynthesis works, (2) an overview of the diversity of photosynthetic organisms, their light harvesting systems, and environmental ranges, (3) a synthesis of photosynthetic surface spectral signatures, and (4) evolutionary rationales for photosynthetic surface reflectance spectra with regard to utilization of photon energy and the planetary light environment. We found the "near-infrared (NIR) end" of the red edge to trend from blue-shifted to reddest for (in order) snow algae, temperate algae, lichens, mosses, aquatic plants, and finally terrestrial vascular plants. The red edge is weak or sloping in lichens. Purple bacteria exhibit possibly a sloping edge in the NIR. More studies are needed on pigment-protein complexes, membrane composition, and measurements of bacteria before firm conclusions can be drawn about the role of the NIR reflectance. Pigment absorbance features are strongly correlated with features of atmospheric spectral transmittance: P680 in Photosystem II with the peak surface incident photon flux density at approximately 685 nm, just before an oxygen band at 687.5 nm; the NIR end of the red edge with water absorbance bands and the oxygen A-band at 761 nm; and bacteriochlorophyll reaction center wavelengths with local maxima in atmospheric and water transmittance spectra. Given the surface incident photon flux density spectrum and resonance transfer in light harvesting, we propose some rules with regard to where photosynthetic pigments will peak in absorbance: (1) the wavelength of peak incident photon flux; (2) the longest available wavelength for core antenna or reaction center pigments; and (3) the shortest wavelengths within an atmospheric window for accessory pigments. That plants absorb less green light may not be an inefficient legacy of evolutionary history, but may actually satisfy the above criteria.

New Triplet Sensitization Routes for Photon Upconversion: Thermally Activated Delayed Fluorescence Molecules, Inorganic Nanocrystals, and Singlet-to-Triplet Absorption.

PubMed

Yanai, Nobuhiro; Kimizuka, Nobuo

2017-10-17

Photon upconversion based on triplet-triplet annihilation (TTA-UC) has attracted much interest because of its possible applications to renewable energy production and biological fields. In particular, the UC of near-infrared (NIR) light to visible (vis) light is imperative to overcome the Shockley-Queisser limit of single-junction photovoltaic cells, and the efficiency of photocatalytic hydrogen production from water can also be improved with the aid of vis-to-ultraviolet (UV) UC. However, both processes have met limitations in the wavelength range, efficiency, and sensitivity for weak incident light. This Account describes recent breakthroughs that solve these major problems, new triplet sensitization routes to significantly enlarge the range of conversion wavelength by minimizing the energy loss during intersystem crossing (ISC) of triplet sensitizers or bypassing the ISC process. The photochemical processes of TTA-UC in general start with the absorption of longer wavelength incident light by triplet sensitizers, which generate the triplet states via ISC. This ISC inevitably accompanies the energy loss of hundreds of millielectronvolts, which significantly limits the TTA-UC with large anti-Stokes shifts. The small S 1 -T 1 gap of molecules showing thermally activated delayed fluorescence (TADF) allows the sensitization of emitters with the highest T 1 and S 1 energy levels ever employed in TTA-UC, which results in efficient vis-to-UV UC. As alternatives to molecular sensitizers in the NIR region, inorganic nanocrystals with broad NIR absorption bands have recently been shown to work as effective sensitizers for NIR-to-vis TTA-UC. Their small exchange splitting minimizes the energy loss during triplet sensitization. The modification of nanocrystal surfaces with organic acceptors via coordination bonds allows efficient energy transfer between the components and succeeding TTA processes. To remove restrictions on the energy loss during ISC, molecules with direct singlet-to-triplet (S-T) excitation are employed as triplet sensitizers. Although the S-T absorption is spin forbidden, large spin-orbital coupling occurs for appropriately designed metal complexes, which allow S-T absorption in the NIR region with large absorption coefficients. While the triplet lifetime of such S-T absorption sensitizers is often short (less than microsecond), the integration of the molecular sensitizers with emitter assemblies allows facile Dexter energy transfer to the surrounding emitter molecules, leading to efficient NIR-to-vis UC emission through triplet energy migration (TEM) in the condensed state. By judicious modification of the chromophore structures, the first example of NIR-to-blue UC has also been achieved. It is essential to combine these new triplet sensitization routes with an upconverted energy collection (UPCON) approach in molecular assemblies to effectively populate emitter triplets and to overcome remaining issues including back energy transfer. We propose two overall materials designs for the TEM-UPCON strategy, core-shell-shell structures and trilayer structures composed of triplet donor, acceptor, and energy collector. The fusion between triplet science and chemistry of self-assembly would overcome previous difficulties of NIR-to-vis and vis-to-UV TTA-UC toward real-world applications ranging from energy to biology.

Imaging using a supercontinuum laser to assess tumors in patients with breast carcinoma

NASA Astrophysics Data System (ADS)

Sordillo, Laura A.; Sordillo, Peter P.; Alfano, R. R.

2016-03-01

The supercontinuum laser light source has many advantages over other light sources, including broad spectral range. Transmission images of paired normal and malignant breast tissue samples from two patients were obtained using a Leukos supercontinuum (SC) laser light source with wavelengths in the second and third NIR optical windows and an IR- CCD InGaAs camera detector (Goodrich Sensors Inc. high response camera SU320KTSW-1.7RT with spectral response between 900 nm and 1,700 nm). Optical attenuation measurements at the four NIR optical windows were obtained from the samples.

Comparisons of spectral aerosol single scattering albedo in Seoul, South Korea

NASA Astrophysics Data System (ADS)

Mok, Jungbin; Krotkov, Nickolay A.; Torres, Omar; Jethva, Hiren; Li, Zhanqing; Kim, Jhoon; Koo, Ja-Ho; Go, Sujung; Irie, Hitoshi; Labow, Gordon; Eck, Thomas F.; Holben, Brent N.; Herman, Jay; Loughman, Robert P.; Spinei, Elena; Lee, Seoung Soo; Khatri, Pradeep; Campanelli, Monica

2018-04-01

Quantifying aerosol absorption at ultraviolet (UV) wavelengths is important for monitoring air pollution and aerosol amounts using current (e.g., Aura/OMI) and future (e.g., TROPOMI, TEMPO, GEMS, and Sentinel-4) satellite measurements. Measurements of column average atmospheric aerosol single scattering albedo (SSA) are performed on the ground by the NASA AERONET in the visible (VIS) and near-infrared (NIR) wavelengths and in the UV-VIS-NIR by the SKYNET networks. Previous comparison studies have focused on VIS and NIR wavelengths due to the lack of co-incident measurements of aerosol and gaseous absorption properties in the UV. This study compares the SKYNET-retrieved SSA in the UV with the SSA derived from a combination of AERONET, MFRSR, and Pandora (AMP) retrievals in Seoul, South Korea, in spring and summer 2016. The results show that the spectrally invariant surface albedo assumed in the SKYNET SSA retrievals leads to underestimated SSA compared to AMP values at near UV wavelengths. Re-processed SKYNET inversions using spectrally varying surface albedo, consistent with the AERONET retrieval improve agreement with AMP SSA. The combined AMP inversions allow for separating aerosol and gaseous (NO2 and O3) absorption and provide aerosol retrievals from the shortest UVB (305 nm) through VIS to NIR wavelengths (870 nm).

Predicting color traits of intact broiler breast fillets using visible and infrared-light

USDA-ARS?s Scientific Manuscript database

The ability of using visible and near-infrared (Vis/NIR) spectroscopy with wavelengths ranging from 400 to 2500nm to predict broiler breast fillets color traits was assessed in this study. Deboning fillets from 214 carcasses slaughtered in a commercial processing plant were included in this work, sp...

Use of Near-Infrared Spectroscopy and Chemometrics for the Nondestructive Identification of Concealed Damage in Raw Almonds (Prunus dulcis).

PubMed

Rogel-Castillo, Cristian; Boulton, Roger; Opastpongkarn, Arunwong; Huang, Guangwei; Mitchell, Alyson E

2016-07-27

Concealed damage (CD) is defined as a brown discoloration of the kernel interior (nutmeat) that appears only after moderate to high heat treatment (e.g., blanching, drying, roasting, etc.). Raw almonds with CD have no visible defects before heat treatment. Currently, there are no screening methods available for detecting CD in raw almonds. Herein, the feasibility of using near-infrared (NIR) spectroscopy between 1125 and 2153 nm for the detection of CD in almonds is demonstrated. Almond kernels with CD have less NIR absorbance in the region related with oil, protein, and carbohydrates. With the use of partial least squares discriminant analysis (PLS-DA) and selection of specific wavelengths, three classification models were developed. The calibration models have false-positive and false-negative error rates ranging between 12.4 and 16.1% and between 10.6 and 17.2%, respectively. The percent error rates ranged between 8.2 and 9.2%. Second-derivative preprocessing of the selected wavelength resulted in the most robust predictive model.

Therapeutic effects of 10-HzPulsed wave lasers in rat depression model: A comparison between near-infrared and red wavelengths.

PubMed

Salehpour, Farzad; Rasta, Seyed Hossein; Mohaddes, Gisou; Sadigh-Eteghad, Saeed; Salarirad, Sima

2016-09-01

The application of transcranial low-level light/laser therapy (tLLLT) in the range of red to near-infrared (NIR) spectrum for psychological disorders is a new area that is attracting growing interest in recent years. The photomodulation effects of NIR and red coherent lights on the activity of cytochrome c oxidase in neuronal cells of brain have been recently introduced. This study, therefore, sought to compare the therapeutic effects of 10-Hz pulsed wave NIR (810 nm) laser with red (630 nm) laser using the same delivered energy density and Citalopram in rat chronic mild stress (CMS) model of depression and anxiety. CMS procedures (for 4 weeks) were used to induce stress. GaAlAs diode laser with red and NIR wavelengths on 10-Hz pulsed wave (50% duty cycle) were used to perform tLLLT treatment for three weeks. An energy density of about 1.2 J/cm-(2) per each session was delivered through a light spot with a diameter of 3-mm to the prefrontal cortex for both wavelengths. Citalopram (10 mg/kg, Intraperitoneal) was administered for twenty-one consecutive days to the drug group. The findings of the present study showed an increase in swimming and decrease in immobility time, for both NIR laser and Citalopram groups compared to the stress group in forced swimming test. Anxiety-like behaviors showed insignificant decrease in all treatment groups in elevated plus maze test. The induction of stress significantly increased serum cortisol levels and treatments with both red laser and Citalopram decreased it. Hyperglycemia induced by CMS returned to normal levels in all treatment groups. The assessment of body weight also showed a significant increase in NIR laser group compared to the stress group by the end of the experiment. This study showed that non-invasive tLLLT using 10-Hz pulsed NIR laser light was as effective as Citalopram and more effective than red laser in the treatment of depressive-like behaviors and may help improve tLLLT as an alternative non-pharmacological treatments of psychological disorders such as depression. Lasers Surg. Med. 48:695-705, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Thermochromic VO2 thin films: solution-based processing, improved optical properties, and lowered phase transformation temperature.

PubMed

Zhang, Zongtao; Gao, Yanfeng; Chen, Zhang; Du, Jing; Cao, Chuanxiang; Kang, Litao; Luo, Hongjie

2010-07-06

This paper describes a solution-phase synthesis of high-quality vanadium dioxide thermochromic thin films. The films obtained showed excellent visible transparency and a large change in transmittance at near-infrared (NIR) wavelengths before and after the metal-insulator phase transition (MIPT). For a 59 nm thick single-layer VO(2) thin film, the integral values of visible transmittance (T(int)) for metallic (M) and semiconductive (S) states were 54.1% and 49.1%, respectively, while the NIR switching efficiencies (DeltaT) were as high as 50% at 2000 nm. Thinner films can provide much higher transmittance of visible light, but they suffer from an attenuation of the switching efficiency in the near-infrared region. By varying the film thickness, ultrahigh T(int) values of 75.2% and 75.7% for the M and S states, respectively, were obtained, while the DeltaT at 2000 nm remained high. These results represent the best data for VO(2) to date. Thicker films in an optimized range can give enhanced NIR switching efficiencies and excellent NIR blocking abilities; in a particularly impressive experiment, one film provided near-zero NIR transmittance in the switched state. The thickness-dependent performance suggests that VO(2) will be of great use in the objective-specific applications. The reflectance and emissivity at the wavelength range of 2.5-25 microm before and after the MIPT were dependent on the film thickness; large contrasts were observed for relatively thick films. This work also showed that the MIPT temperature can be reduced simply by selecting the annealing temperature that induces local nonstoichiometry; a MIPT temperature as low as 42.7 degrees C was obtained by annealing the film at 440 degrees C. These properties (the high visible transmittance, the large change in infrared transmittance, and the near room-temperature MIPT) suggest that the current method is a landmark in the development of this interesting material toward applications in energy-saving smart windows.

Single-pulse picking at kHz repetition rates using a Ge plasma switch at the free-electron laser FELBE.

PubMed

Schmidt, J; Winnerl, S; Seidel, W; Bauer, C; Gensch, M; Schneider, H; Helm, M

2015-06-01

We demonstrate a system for picking of mid-infrared and terahertz (THz) radiation pulses from the free-electron laser (FEL) FELBE operating at a repetition rate of 13 MHz. Single pulses are reflected by a dense electron-hole plasma in a Ge slab that is photoexcited by amplified near-infrared (NIR) laser systems operating at repetition rates of 1 kHz and 100 kHz, respectively. The peak intensity of picked pulses is up to 400 times larger than the peak intensity of residual pulses. The required NIR fluence for picking pulses at wavelengths in the range from 5 μm to 30 μm is discussed. In addition, we show that the reflectivity of the plasma decays on a time scale from 100 ps to 1 ns dependent on the wavelengths of the FEL and the NIR laser. The plasma switch enables experiments with the FEL that require high peak power but lower average power. Furthermore, the system is well suited to investigate processes with decay times in the μs to ms regime, i.e., much longer than the 77 ns long pulse repetition period of FELBE.

Measurements of liquid film thickness, concentration, and temperature of aqueous urea solution by NIR absorption spectroscopy

NASA Astrophysics Data System (ADS)

Pan, R.; Jeffries, J. B.; Dreier, T.; Schulz, C.

2016-01-01

A multi-wavelength near-infrared (NIR) diode laser absorption sensor has been developed and demonstrated for real-time monitoring of the thickness, solute concentration, and temperature of thin films of urea-water solutions. The sensor monitors the transmittance of three near-infrared diode lasers through the thin liquid film. Film thickness, urea mass fraction, and liquid temperature were determined from measured transmittance ratios of suitable combinations of lasers. Available laser wavelengths were selected depending on the variation of the NIR absorption spectrum of the solution with temperature and solute concentration. The spectral database was measured by a Fourier transform infrared spectrometer in the range 5500-8000 cm-1 for urea solutions between 5 and 40 wt% and temperatures between 298 and 338 K. A prototype sensor was constructed, and the sensor concept was first validated with measurements using a calibration cell providing liquid layers of variable thickness (200-1500 µm), urea mass fraction (5-40 wt%) and temperature (298-318 K). Temporal variations of film thickness and urea concentration were captured during the constant-temperature evaporation of a liquid film deposited on an optically polished heated quartz flat.

Single-pulse picking at kHz repetition rates using a Ge plasma switch at the free-electron laser FELBE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmidt, J., E-mail: j.schmidt@hzdr.de; Helm, M.; Technische Universität Dresden, 01062 Dresden

2015-06-15

We demonstrate a system for picking of mid-infrared and terahertz (THz) radiation pulses from the free-electron laser (FEL) FELBE operating at a repetition rate of 13 MHz. Single pulses are reflected by a dense electron-hole plasma in a Ge slab that is photoexcited by amplified near-infrared (NIR) laser systems operating at repetition rates of 1 kHz and 100 kHz, respectively. The peak intensity of picked pulses is up to 400 times larger than the peak intensity of residual pulses. The required NIR fluence for picking pulses at wavelengths in the range from 5 μm to 30 μm is discussed. Inmore » addition, we show that the reflectivity of the plasma decays on a time scale from 100 ps to 1 ns dependent on the wavelengths of the FEL and the NIR laser. The plasma switch enables experiments with the FEL that require high peak power but lower average power. Furthermore, the system is well suited to investigate processes with decay times in the μs to ms regime, i.e., much longer than the 77 ns long pulse repetition period of FELBE.« less

Comparison of visible and near infrared reflectance spectroscopy on fat to authenticate dietary history of lambs.

PubMed

Huang, Y; Andueza, D; de Oliveira, L; Zawadzki, F; Prache, S

2015-11-01

Since consumers are showing increased interest in the origin and method of production of their food, it is important to be able to authenticate dietary history of animals by rapid and robust methods used in the ruminant products. Promising breakthroughs have been made in the use of spectroscopic methods on fat to discriminate pasture-fed and concentrate-fed lambs. However, questions remained on their discriminatory ability in more complex feeding conditions, such as concentrate-finishing after pasture-feeding. We compared the ability of visible reflectance spectroscopy (Vis RS, wavelength range: 400 to 700 nm) with that of visible-near-infrared reflectance spectroscopy (Vis-NIR RS, wavelength range: 400 to 2500 nm) to differentiate between carcasses of lambs reared with three feeding regimes, using partial least square discriminant analysis (PLS-DA) as a classification method. The sample set comprised perirenal fat of Romane male lambs fattened at pasture (P, n = 69), stall-fattened indoors on commercial concentrate and straw (S, n = 55) and finished indoors with concentrate and straw for 28 days after pasture-feeding (PS, n = 65). The overall correct classification rate was better for Vis-NIR RS than for Vis RS (99.0% v. 95.1%, P < 0.05). Vis-NIR RS allowed a correct classification rate of 98.6%, 100.0% and 98.5% for P, S and PS lambs, respectively, whereas Vis RS allowed a correct classification rate of 98.6%, 94.5% and 92.3% for P, S and PS lambs, respectively. This study suggests the likely implication of molecules absorbing light in the non-visible part of the Vis-NIR spectra (possibly fatty acids), together with carotenoid and haem pigments, in the discrimination of the three feeding regimes.

Effects of grown origin, genotype, harvest year, and their interactions of wheat kernels on near infrared spectral fingerprints for geographical traceability.

PubMed

Zhao, Haiyan; Guo, Boli; Wei, Yimin; Zhang, Bo

2014-01-01

The effects of origin, genotype, harvest year, and their interactions on wheat near infrared (NIR) spectra were studied to find the reasons for differences in NIR fingerprints of wheat from different geographical origins and the stability of NIR fingerprints among different years. Ten varieties were grown in three regions of China for 2 years. 180 kernel samples were analysed by NIR. The spectra after pre-treatment were analysed by principal component analysis, multi-way analysis of variance, and discriminant partial least-squares. The results showed that origin, genotype, year, and their interactions all had significant effects on wheat NIR fingerprints. The second overtones of N-H and C-H stretching vibrations and a combination of stretch and deformation of C-H group in wheat were mainly influenced by the geographical origin. The wavelength ranges 975-990 nm, 1200 nm, and 1355-1380 nm contained plenty of origin information to build robust discriminant models of wheat geographical origin. Copyright © 2013 Elsevier Ltd. All rights reserved.

Upconverting fluorescent nanoparticles for biodetection and photoactivation

NASA Astrophysics Data System (ADS)

Huang, Kai; Li, WenKai; Jayakumar, Muthu Kumara Gnanasammandhan; Zhang, Yong

2013-03-01

Fluorophores including fluorescent dyes/proteins and quantum dots (QDs) are used for fluorescence-based imaging and detection. These are based on `downconversion fluorescence' and have several drawbacks: photobleaching, autofluorescence, short tissue penetration depth and tissue photo-damage. Upconversion fluorescent nanoparticles (UCNs) emit detectable photons of higher energy in the short wavelength range upon irradiation with near-infrared (NIR) light based on a process termed `upconversion'. UCNs show absolute photostability, negligible autofluorescence, high penetration depth and minimum photodamage to biological tissues. Lanthanide doped nanocrystals with nearinfrared NIR-to-NIR and/or NIR-to-VIS and/or NIR-to-UV upconversion fluorescence emission have been synthesized. The nanocrystals with small size and tunable multi-color emission have been developed. The emission can be tuned by doping different upconverting lanthanide ions into the nanocrystals. The nanocrystals with core-shell structure have also been prepared to tune the emission color. The surfaces of these nanocrystals have been modified to render them water dispersible and biocompatible. They can be used for ultrasensitive interference-free biodetection because most biomolecules do not have upconversion properties. UCNs are also useful for light based therapy with enhanced efficiency, for example, photoactivation.

Feasibility of using a miniature NIR spectrometer to measure volumic mass during alcoholic fermentation.

PubMed

Fernández-Novales, Juan; López, María-Isabel; González-Caballero, Virginia; Ramírez, Pilar; Sánchez, María-Teresa

2011-06-01

Volumic mass-a key component of must quality control tests during alcoholic fermentation-is of great interest to the winemaking industry. Transmitance near-infrared (NIR) spectra of 124 must samples over the range of 200-1,100-nm were obtained using a miniature spectrometer. The performance of this instrument to predict volumic mass was evaluated using partial least squares (PLS) regression and multiple linear regression (MLR). The validation statistics coefficient of determination (r(2)) and the standard error of prediction (SEP) were r(2) = 0.98, n = 31 and r(2) = 0.96, n = 31, and SEP = 5.85 and 7.49 g/dm(3) for PLS and MLR equations developed to fit reference data for volumic mass and spectral data. Comparison of results from MLR and PLS demonstrates that a MLR model with six significant wavelengths (P < 0.05) fit volumic mass data to transmittance (1/T) data slightly worse than a more sophisticated PLS model using the full scanning range. The results suggest that NIR spectroscopy is a suitable technique for predicting volumic mass during alcoholic fermentation, and that a low-cost NIR instrument can be used for this purpose.

Transmission near-infrared (NIR) and photon time-of-flight (PTOF) spectroscopy in a comparative analysis of pharmaceuticals.

PubMed

Kamran, Faisal; Abildgaard, Otto H A; Sparén, Anders; Svensson, Olof; Johansson, Jonas; Andersson-Engels, Stefan; Andersen, Peter E; Khoptyar, Dmitry

2015-03-01

We present a comprehensive study of the application of photon time-of-flight spectroscopy (PTOFS) in the wavelength range 1050-1350 nm as a spectroscopic technique for the evaluation of the chemical composition and structural properties of pharmaceutical tablets. PTOFS is compared to transmission near-infrared spectroscopy (NIRS). In contrast to transmission NIRS, PTOFS is capable of directly and independently determining the absorption and reduced scattering coefficients of the medium. Chemometric models were built on the evaluated absorption spectra for predicting tablet drug concentration. Results are compared to corresponding predictions built on transmission NIRS measurements. The predictive ability of PTOFS and transmission NIRS is comparable when models are based on uniformly distributed tablet sets. For non-uniform distribution of tablets based on particle sizes, the prediction ability of PTOFS is better than that of transmission NIRS. Analysis of reduced scattering spectra shows that PTOFS is able to characterize tablet microstructure and manufacturing process parameters. In contrast to the chemometric pseudo-variables provided by transmission NIRS, PTOFS provides physically meaningful quantities such as scattering strength and slope of particle size. The ability of PTOFS to quantify the reduced scattering spectra, together with its robustness in predicting drug content, makes it suitable for such evaluations in the pharmaceutical industry.

Photothermal effects from Au-Cu2O core-shell nanocubes, octahedra, and nanobars with broad near-infrared absorption tunability

NASA Astrophysics Data System (ADS)

Wang, Hsiang-Ju; Yang, Kung-Hsun; Hsu, Shih-Chen; Huang, Michael H.

2015-12-01

Other than the display of purely optical phenomenon, the recently-discovered facet-dependent optical properties of metal-Cu2O nanocrystals have become useful by illuminating Au-Cu2O nanocubes and octahedra having a surface plasmon resonance (SPR) absorption band in the near-infrared (NIR) region from octahedral Au cores with 808 nm light for heat generation. After 5 min of light irradiation, a solution of Au-Cu2O nanocubes can reach 65 °C with their Au SPR band matching the illuminating light wavelength. Photothermal efficiency has been found to be facet-dependent. In addition, short gold nanorods were employed to synthesize {100}-bound rectangular Au-Cu2O nanobars with a tunable longitudinal Au SPR absorption band covering a broad NIR range from ~1050 to 1400 nm. Because the Au SPR bands can become fixed with relatively thin Cu2O shells of less than 15 nm, ultrasmall nanobars having a size of 61 nm directly red-shift the Au SPR band to 1047 nm. And 73 nm nanobars can give a Au SPR band at 1390 nm. Truncated nanobars exposing {100}, {110}, and {111} facets give a very blue-shifted Au SPR band. The nanobars also exhibit photothermal activity when illuminated by 1064 nm light. These small Au-Cu2O nanocrystals represent the simplest nanostructure design to absorb light covering the entire NIR wavelengths.Other than the display of purely optical phenomenon, the recently-discovered facet-dependent optical properties of metal-Cu2O nanocrystals have become useful by illuminating Au-Cu2O nanocubes and octahedra having a surface plasmon resonance (SPR) absorption band in the near-infrared (NIR) region from octahedral Au cores with 808 nm light for heat generation. After 5 min of light irradiation, a solution of Au-Cu2O nanocubes can reach 65 °C with their Au SPR band matching the illuminating light wavelength. Photothermal efficiency has been found to be facet-dependent. In addition, short gold nanorods were employed to synthesize {100}-bound rectangular Au-Cu2O nanobars with a tunable longitudinal Au SPR absorption band covering a broad NIR range from ~1050 to 1400 nm. Because the Au SPR bands can become fixed with relatively thin Cu2O shells of less than 15 nm, ultrasmall nanobars having a size of 61 nm directly red-shift the Au SPR band to 1047 nm. And 73 nm nanobars can give a Au SPR band at 1390 nm. Truncated nanobars exposing {100}, {110}, and {111} facets give a very blue-shifted Au SPR band. The nanobars also exhibit photothermal activity when illuminated by 1064 nm light. These small Au-Cu2O nanocrystals represent the simplest nanostructure design to absorb light covering the entire NIR wavelengths. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr06847a

Method of predicting mechanical properties of decayed wood

DOEpatents

Kelley, Stephen S.

2003-07-15

A method for determining the mechanical properties of decayed wood that has been exposed to wood decay microorganisms, comprising: a) illuminating a surface of decayed wood that has been exposed to wood decay microorganisms with wavelengths from visible and near infrared (VIS-NIR) spectra; b) analyzing the surface of the decayed wood using a spectrometric method, the method generating a first spectral data of wavelengths in VIS-NIR spectra region; and c) using a multivariate analysis to predict mechanical properties of decayed wood by comparing the first spectral data with a calibration model, the calibration model comprising a second spectrometric method of spectral data of wavelengths in VIS-NIR spectra obtained from a reference decay wood, the second spectral data being correlated with a known mechanical property analytical result obtained from the reference decayed wood.

GIARPS@TNG: GIANO-B and HARPS-N together for a wider wavelength range spectroscopy

NASA Astrophysics Data System (ADS)

Claudi, R.; Benatti, S.; Carleo, I.; Ghedina, A.; Guerra, J.; Micela, G.; Molinari, E.; Oliva, E.; Rainer, M.; Tozzi, A.; Baffa, C.; Baruffolo, A.; Buchschacher, N.; Cecconi, M.; Cosentino, R.; Fantinel, D.; Fini, L.; Ghinassi, F.; Giani, E.; Gonzalez, E.; Gonzalez, M.; Gratton, R.; Harutyunyan, A.; Hernandez, N.; Lodi, M.; Malavolta, L.; Maldonado, J.; Origlia, L.; Sanna, N.; Sanjuan, J.; Scuderi, S.; Seemann, U.; Sozzetti, A.; Perez Ventura, H.; Hernandez Diaz, M.; Galli, A.; Gonzalez, C.; Riverol, L.; Riverol, C.

2017-08-01

Since 2012, thanks to the installation of the high-resolution echelle spectrograph in the optical range HARPS-N, the Italian telescope TNG (La Palma) became one of the key facilities for the study of the extrasolar planets. In 2014 TNG also offered GIANO to the scientific community, providing a near-infrared (NIR) cross-dispersed echelle spectroscopy covering 0.97-2.45μm at a resolution of 50000. GIANO, although designed for direct light-feed from the telescope at the Nasmyth-B focus, was provisionally mounted on the rotating building and connected via fibers to only available interface at the Nasmyth-A focal plane. The synergy between these two instruments is particularly appealing for a wide range of science cases, especially for the search of exoplanets around young and active stars and the characterisation of their atmosphere. Through the funding scheme "WOW" (a Way to Others Worlds), the Italian National Institute for Astrophysics (INAF) proposed to position GIANO at the focal station for which it was originally designed and the simultaneous use of these spectrographs with the aim to achieve high-resolution spectroscopy in a wide wavelength range (0.383-2.45μm) obtained in a single exposure, giving rise to the project called GIARPS (GIANO-B & HARPS-N). Because of its characteristics, GIARPS can be considered the first and unique worldwide instrument providing not only high resolution in a large wavelength band, but also a high-precision radial velocity measurement both in the visible and in the NIR arm, since in the next future GIANO-B will be equipped with gas absorption cells.

Ring-patterned plasmonic photonic crystal thermal light source for miniaturized near-infrared spectrometers

NASA Astrophysics Data System (ADS)

Labib, Shady R.; Elsayed, Ahmed A.; Sabry, Yasser M.; Khalil, Diaa

2018-02-01

There is a growing number of spectroscopy applications in the near-infrared (NIR) range including gas sensing, food analysis, pharmaceutical and industrial applications that requires highly efficient, more compact and low-cost miniaturized spectrometers. One of the key components for such systems is the wideband light source that can be fabricated using Silicon technology and hence integrated with other components on the same chip. In this work, we report a ring-patterned plasmonic photonic crystal (PC) thermal light source for miniaturized near-infrared spectrometers. The design is based on silicon and tuned to achieve wavelength selectivity in the emitted spectrum. The design is optimized by using Rigorous Coupled-Wave Analysis (RCWA) simulation, which is used to compute the power reflectance and transmittance that are used to predict the emissivity of the structure. The design consists of a PC of silicon rings coated with platinum. The period of the structure is about 2 μm and the silicon is highly-doped with n-type doping level in the order of 1019-1020 cm-3 to enhance the free-carrier absorption. The ring etching depth, diameter and shell thickness are optimized to increase its emissivity within a specific wavelength range of interest. The simulation results show an emissivity exceeding 0.9 in the NIR range up to 2.5 μm, while the emissivity is decreased significantly for longer wavelengths suppressing the emission out of the range of interest, and hence increasing the efficiency for the source. The reported results open the door for black body radiation engineering in integrated silicon sources for spectrometer miniaturization.

High frame-rate MR-guided near-infrared tomography system to monitor breast hemodynamics

NASA Astrophysics Data System (ADS)

Li, Zhiqiu; Jiang, Shudong; Krishnaswamy, Venkataramanan; Davis, Scott C.; Srinivasan, Subhadra; Paulsen, Keith D.; Pogue, Brian W.

2011-02-01

A near-infrared (NIR) tomography system with spectral-encoded sources at two wavelength bands was built to quantify the temporal contrast at 20 Hz bandwidth, while imaging breast tissue. The NIR system was integrated with a magnetic resonance (MR) machine through a custom breast coil interface, and both NIR data and MR images were acquired simultaneously. MR images provided breast tissue structural information for NIR reconstruction. Acquisition of finger pulse oximeter (PO) plethysmogram was synchronized with the NIR system in the experiment to offer a frequency-locked reference. The recovered absorption coefficients of the breast at two wavelengths showed identical temporal frequency as the PO output, proving this multi-modality design can recover the small pulsatile variation of absorption property in breast tissue related to the heartbeat. And it also showed the system's ability on novel contrast imaging of fast flow signals in deep tissue.

Indocyanine green fluorescence in second near-infrared (NIR-II) window

PubMed Central

Bhavane, Rohan; Ghaghada, Ketan B.; Vasudevan, Sanjeev A.; Kaay, Alexander; Annapragada, Ananth

2017-01-01

Indocyanine green (ICG), a FDA approved near infrared (NIR) fluorescent agent, is used in the clinic for a variety of applications including lymphangiography, intra-operative lymph node identification, tumor imaging, superficial vascular imaging, and marking ischemic tissues. These applications operate in the so-called “NIR-I” window (700–900 nm). Recently, imaging in the “NIR-II” window (1000–1700 nm) has attracted attention since, at longer wavelengths, photon absorption, and scattering effects by tissue components are reduced, making it possible to image deeper into the underlying tissue. Agents for NIR-II imaging are, however, still in pre-clinical development. In this study, we investigated ICG as a NIR-II dye. The absorbance and NIR-II fluorescence emission of ICG were measured in different media (PBS, plasma and ethanol) for a range of ICG concentrations. In vitro and in vivo testing were performed using a custom-built spectral NIR assembly to facilitate simultaneous imaging in NIR-I and NIR-II window. In vitro studies using ICG were performed using capillary tubes (as a simulation of blood vessels) embedded in Intralipid solution and tissue phantoms to evaluate depth of tissue penetration in NIR-I and NIR-II window. In vivo imaging using ICG was performed in nude mice to evaluate vascular visualization in the hind limb in the NIR-I and II windows. Contrast-to-noise ratios (CNR) were calculated for comparison of image quality in NIR-I and NIR-II window. ICG exhibited significant fluorescence emission in the NIR-II window and this emission (similar to the absorption profile) is substantially affected by the environment of the ICG molecules. In vivo imaging further confirmed the utility of ICG as a fluorescent dye in the NIR-II domain, with the CNR values being ~2 times those in the NIR-I window. The availability of an FDA approved imaging agent could accelerate the clinical translation of NIR-II imaging technology. PMID:29121078

Synergistic thermoradiotherapy based on PEGylated Cu3BiS3 ternary semiconductor nanorods with strong absorption in the second near-infrared window.

PubMed

Li, Ang; Li, Xiang; Yu, Xujiang; Li, Wei; Zhao, Ruyi; An, Xiao; Cui, Daxiang; Chen, Xiaoyuan; Li, Wanwan

2017-01-01

In this work, we report a successful synthesis of copper bismuth sulfide nanorods (NRs) with broad and strong photoabsorption ranging from ultraviolet (UV) to near-infrared (NIR) wavelengths, which can be used as a 1064 nm-laser-driven photothermal agent with the photothermal conversion efficiency of 40.7%, noticeably higher than most of the reported PTT agents working in NIR-II window. The as-prepared PEGylated Cu 3 BiS 3 NRs were used as photoacoustic imaging (PAI) and CT imaging agents due to their strong NIR absorption and large X-ray attenuation coefficient of bismuth. We are the first to demonstrate that a small quantity of PEGylated Cu 3 BiS 3 NRs in tumors can concentrate radiation energy and trigger mild PTT under NIR-II irradiation and thus, these particles could be used as a novel, synergistic thermoradiotheraputic agent that enhances the efficacy of radiotherapy. Copyright © 2016 Elsevier Ltd. All rights reserved.

CuS/RGO hybrid photocatalyst for full solar spectrum photoreduction from UV/Vis to near-infrared light.

PubMed

Wu, Jie; Liu, Baibai; Ren, Zhenxing; Ni, Mengying; Li, Can; Gong, Yinyan; Qin, Wei; Huang, Yongli; Sun, Chang Q; Liu, Xinjuan

2018-05-01

To make full use of the solar energy, it remains a great challenge for semiconductor photocatalysts to harvest the full solar light spectrum from ultraviolet (UV) to visible even the near infrared (NIR) wavelength. Here we show firstly the CuS/RGO (reduced graphene oxide) hybrid photocatalyst synthesized via a microwave assisted method with full solar light (UV-Vis-NIR) active for efficient Cr(VI) reduction. The CuS/RGO displays high absorption and catalytic activity in the UV, visible and even the NIR light regions. As co-catalyst, RGO can separate and inhibit the recombination of charge carriers, consequently improving the catalytic activity. Only 1wt% RGO emersions can reduce 90% of Cr(VI) under the radiation of light over the full spectrum. Findings may provide a new strategy and substance to expand the utilization range of solar light from UV to visible even the NIR energy. Copyright © 2017. Published by Elsevier Inc.

Feasibility of detecting aflatoxin B1 on inoculated maize kernels surface using Vis/NIR hyperspectral imaging

USDA-ARS?s Scientific Manuscript database

The feasibility of using a visible/near-infrared hyperspectral imaging system with a wavelength range between 400 and 1000 nm to detect and differentiate different levels of aflatoxin B1 (AFB1) artificially titrated on maize kernel surface was examined. To reduce the color effects of maize kernels, ...

AN ANALYSIS OF THE SHAPES OF INTERSTELLAR EXTINCTION CURVES. VI. THE NEAR-IR EXTINCTION LAW

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fitzpatrick, E. L.; Massa, D.

We combine new observations from the Hubble Space Telescope's Advanced Camera of Survey with existing data to investigate the wavelength dependence of near-IR (NIR) extinction. Previous studies suggest a power law form for NIR extinction, with a 'universal' value of the exponent, although some recent observations indicate that significant sight line-to-sight line variability may exist. We show that a power-law model for the NIR extinction provides an excellent fit to most extinction curves, but that the value of the power, {beta}, varies significantly from sight line to sight line. Therefore, it seems that a 'universal NIR extinction law' is notmore » possible. Instead, we find that as {beta} decreases, R(V) {identical_to} A(V)/E(B - V) tends to increase, suggesting that NIR extinction curves which have been considered 'peculiar' may, in fact, be typical for different R(V) values. We show that the power-law parameters can depend on the wavelength interval used to derive them, with the {beta} increasing as longer wavelengths are included. This result implies that extrapolating power-law fits to determine R(V) is unreliable. To avoid this problem, we adopt a different functional form for NIR extinction. This new form mimics a power law whose exponent increases with wavelength, has only two free parameters, can fit all of our curves over a longer wavelength baseline and to higher precision, and produces R(V) values which are consistent with independent estimates and commonly used methods for estimating R(V). Furthermore, unlike the power-law model, it gives R(V)s that are independent of the wavelength interval used to derive them. It also suggests that the relation R(V) = -1.36 E(K-V)/(E(B-V)) - 0.79 can estimate R(V) to {+-}0.12. Finally, we use model extinction curves to show that our extinction curves are in accord with theoretical expectations, and demonstrate how large samples of observational quantities can provide useful constraints on the grain properties.« less

Potential of Visible and Near Infrared Spectroscopy and Pattern Recognition for Rapid Quantification of Notoginseng Powder with Adulterants

PubMed Central

Nie, Pengcheng; Wu, Di; Sun, Da-Wen; Cao, Fang; Bao, Yidan; He, Yong

2013-01-01

Notoginseng is a classical traditional Chinese medical herb, which is of high economic and medical value. Notoginseng powder (NP) could be easily adulterated with Sophora flavescens powder (SFP) or corn flour (CF), because of their similar tastes and appearances and much lower cost for these adulterants. The objective of this study is to quantify the NP content in adulterated NP by using a rapid and non-destructive visible and near infrared (Vis-NIR) spectroscopy method. Three wavelength ranges of visible spectra, short-wave near infrared spectra (SNIR) and long-wave near infrared spectra (LNIR) were separately used to establish the model based on two calibration methods of partial least square regression (PLSR) and least-squares support vector machines (LS-SVM), respectively. Competitive adaptive reweighted sampling (CARS) was conducted to identify the most important wavelengths/variables that had the greatest influence on the adulterant quantification throughout the whole wavelength range. The CARS-PLSR models based on LNIR were determined as the best models for the quantification of NP adulterated with SFP, CF, and their mixtures, in which the rP values were 0.940, 0.939, and 0.867 for the three models respectively. The research demonstrated the potential of the Vis-NIR spectroscopy technique for the rapid and non-destructive quantification of NP containing adulterants. PMID:24129019

VizieR Online Data Catalog: HD147379 b velocity curve (Reiners+, 2018)

NASA Astrophysics Data System (ADS)

Reiners, A.; Ribas, I.; Zechmeister, M.; Caballero, J. A.; Trifonov, T.; Dreizler, S.; Morales, J. C.; Tal-Or, L.; Lafarga, M.; Quirrenbach, A.; Amado, P. J.; Kaminski, A.; Jeffers, S. V.; Aceituno, J.; Bejar, V. J. S.; Guardia, J.; Guenther, E. W.; Hagen, H.-J.; Montes, D.; Passegger, V. M.; Seifert, W.; Schweitzer, A.; Cortes-Contreras, M.; Abril, M.; Alonso-Floriano, F. J.; Ammler-von Eiff, M.; Antona, R.; Anglada-Escude, G.; Anwand-Heerwart, H.; Arroyo-Torres, B.; Azzaro, M.; Baroch, D.; Barrado, D.; Bauer, F. F.; Becerril, S.; Benitez, D.; Berdinas, Z. M.; Bergond, G.; Bluemcke, M.; Brinkmoeller, M.; Del Burgo, C.; Cano, J.; Cardenas Vazquez, M. C.; Casal, E.; Cifuentes, C.; Claret, A.; Colome, J.; Czesla, S.; Diez-Alonso, E.; Feiz, C.; Fernandez, M.; Ferro, I. M.; Fuhrmeister, B.; Galadi-Enriquez, D.; Garcia-Piquer, A.; Garcia Vargas, M. L.; Gesa, L.; Gomez Galera, V.; Gonzalez Hernandez, J. I.; Gonzalez-Peinado, R.; Groezinger, U.; Grohnert, S.; Guijarro, A.; de Guindos, E.; Gutierrez-Soto, J.; Hatzes, A. P.; Hauschildt, P. H.; Hedrosa, R. P.; Helml!, Ing J.; H Enning, Th.; Hermelo, I.; Hernandez Arabi, R.; Hernandez Castano, L.; Hernandez Hernando, F.; Herrero, E.; Huber, A.; Huke, P.; Johnson, E. N.; de Juan, E.; Kim, M.; Klein, R.; Klueter, J.; Klutsch, A.; Kuerster, M.; Labarga, F.; Lamert, A.; Lampon, M.; Lara, L. M.; Laun, W.; Lemke, U.; Lenzen, R.; Launhardt, R.; Lopez Del Fresno, M.; Lopez-Gonzalez, M. J.; Lopez-Puertas, M.; Lopez Salas, J. F.; Lopez-Santiago, J.; Luque, R.; Magan Madinabeitia, H.; Mall, U.; Mancini, L.; Mandel, H.; Marfil, E.; Marin Molina, J. A.; Maroto Fernandez, D.; Martin, E. L.; Martin-Ruiz, S.; Marvin, C. J.; Mathar, R. J.; Mirabet, E.; Moreno-Raya, M. E.; Moya, A.; Mundt, R.; Nagel, E.; Naranjo, V.; Nortmann, L.; Nowak, G.; Ofir, A.; Oreiro, R.; Palle, E.; Panduro, J.; Pascual, J.; Pavlov, A.; Pedraz, S.; Perez-Calpena, A.; Perez Medialdea, D.; Perger, M.; Perryman, M. A. C.; Pluto, M.; Rabaza, O.; Ramon, A.; Rebolo, R.; Redondo, P.; Reffert, S.; Reinhart, S.; Rhode, P.; Rix, H.-W.; Rodler, F.; Rodriguez, E.; Rodriguez-Lopez, C.; Rodriguez Trinidad, A.; Rohloff, R.-R.; Rosich, A.; ! Sadegi, S.; Sanchez-Blanco, E.; Sanchez Carrasco, M. A.; Sanchez-Lopez, A.; Sanz-Forcada, J.; Sarkis, P.; Sarmiento, L. F.; Schaefer, S.; Schmitt, J. H. M. M.; Schiller, J.; Schoefer, P.; Solano, E.; Stahl, O.; Strachan, J. B. P.; Stuermer, J.; Suarez, J. C.; Tabernero, H. M.; Tala, M.; Tulloch, S. M.; Ulbrich, R.-G.; Veredas, G.; Vico Linares, J. I.; Vilardell, F.; Wagner, K.; Winkler, J.; Wolthoff, V.; Xu, W.; Yan, F.; Zapatero Osorio, M. R.

2017-11-01

We analyzed data from the CARMENES VIS channel and HIRES/Keck. The CARMENES measurements were taken in the context of the CARMENES search for exoplanets around M dwarfs. The CARMENES instrument consists of two channels: the VIS channel obtains spectra at a resolution of R=94600 in the wavelength range 520-960nm, while the NIR channel yields spectra of R=80400 covering 960-1710nm. Both channels are calibrated in wavelength with hollow-cathode lamps and use temperature- and pressure-stabilized Fabry-Perot etalons to interpolate the wavelength solution and simultaneously monitor the spectrograph drift during nightly operations (Bauer et al., 2015A&A...581A.117B). (1 data file).

Autofluorescence Imaging With Near-Infrared Excitation:Normalization by Reflectance to Reduce Signal From Choroidal Fluorophores

PubMed Central

Cideciyan, Artur V.; Swider, Malgorzata; Jacobson, Samuel G.

2015-01-01

Purpose. We previously developed reduced-illuminance autofluorescence imaging (RAFI) methods involving near-infrared (NIR) excitation to image melanin-based fluorophores and short-wavelength (SW) excitation to image lipofuscin-based flurophores. Here, we propose to normalize NIR-RAFI in order to increase the relative contribution of retinal pigment epithelium (RPE) fluorophores. Methods. Retinal imaging was performed with a standard protocol holding system parameters invariant in healthy subjects and in patients. Normalized NIR-RAFI was derived by dividing NIR-RAFI signal by NIR reflectance point-by-point after image registration. Results. Regions of RPE atrophy in Stargardt disease, AMD, retinitis pigmentosa, choroideremia, and Leber congenital amaurosis as defined by low signal on SW-RAFI could correspond to a wide range of signal on NIR-RAFI depending on the contribution from the choroidal component. Retinal pigment epithelium atrophy tended to always correspond to high signal on NIR reflectance. Normalizing NIR-RAFI reduced the choroidal component of the signal in regions of atrophy. Quantitative evaluation of RPE atrophy area showed no significant differences between SW-RAFI and normalized NIR-RAFI. Conclusions. Imaging of RPE atrophy using lipofuscin-based AF imaging has become the gold standard. However, this technique involves bright SW lights that are uncomfortable and may accelerate the rate of disease progression in vulnerable retinas. The NIR-RAFI method developed here is a melanin-based alternative that is not absorbed by opsins and bisretinoid moieties, and is comfortable to view. Further development of this method may result in a nonmydriatic and comfortable imaging method to quantify RPE atrophy extent and its expansion rate. PMID:26024124

Fluorescence detection of glutathione and oxidized glutathione in blood with a NIR-excitable cyanine probe

NASA Astrophysics Data System (ADS)

Liu, Chang-hui; Qi, Feng-pei; Wen, Fu-bin; Long, Li-ping; Liu, Ai-juan; Yang, Rong-hua

2018-04-01

Cyanine has been widely utilized as a near infrared (NIR) fluorophore for detection of glutathione (GSH). However, the excitation of most of the reported cyanine-based probes was less than 800 nm, which inevitably induce biological background absorption and lower the sensitivity, limiting their use for detection of GSH in blood samples. To address this issue, here, a heptamethine cyanine probe (DNIR), with a NIR excitation wavelength at 804 nm and a NIR emission wavelength at 832 nm, is employed for the detection of GSH and its oxidized form (GSSG) in blood. The probe displays excellent selectivity for GSH over GSSG and other amino acids, and rapid response to GSH, in particular a good property for indirect detection of GSSG in the presence of enzyme glutathione reductase and the reducing agent nicotinamideadenine dinucleotide phosphate, without further separation prior to fluorescent measurement. To the best of our knowledge, this is the first attempt to explore NIR fluorescent approach for the simultaneous assay of GSH and GSSG in blood. As such, we expect that our fluorescence sensors with both NIR excitation and NIR emission make this strategy suitable for the application in complex physiological systems.

NIR small arms muzzle flash

NASA Astrophysics Data System (ADS)

Montoya, Joseph; Kennerly, Stephen; Rede, Edward

2010-04-01

Utilization of Near-Infrared (NIR) spectral features in a muzzle flash will allow for small arms detection using low cost silicon (Si)-based imagers. Detection of a small arms muzzle flash in a particular wavelength region is dependent on the intensity of that emission, the efficiency of source emission transmission through the atmosphere, and the relative intensity of the background scene. The NIR muzzle flash signature exists in the relatively large Si spectral response wavelength region of 300 nm-1100 nm, which allows for use of commercial-off-the-shelf (COTS) Si-based detectors. The alkali metal origin of the NIR spectral features in the 7.62 × 39-mm round muzzle flash is discussed, and the basis for the spectral bandwidth is examined, using a calculated Voigt profile. This report will introduce a model of the 7.62 × 39-mm NIR muzzle flash signature based on predicted source characteristics. Atmospheric limitations based on NIR spectral regions are investigated in relation to the NIR muzzle flash signature. A simple signal-to-clutter ratio (SCR) metric is used to predict sensor performance based on a model of radiance for the source and solar background and pixel registered image subtraction.

Experimental Near Infrared Spectroscopy of Polycyclic Aromatic Hydrocarbons Between 0.7 to 2.5 microns

NASA Technical Reports Server (NTRS)

Mattioda, A. L.; Hudgins, D. M.; Allamandola, L. J.

2005-01-01

The near infrared (NIR) spectra and absolute band strengths of 27 polycyclic aromatic hydrocarbon (PAH) cations and anions ranging in size from C14H10 to C50H22, are reported. The spectra from 0.7 to 2.5 microns (14,000 to 4000/cm) are presented for the fifteen PAHs ranging in size from C40H18 to C50H22 whereas the spectra of the remaining twelve span the narrower range from 0.7 to 1.1 microns (14,000 to 9000/cm). The spectra of all the ionized PAHs we have studied to date have strong, broad absorption bands in the NIR arising from electronic transitions. This work shows that ionized PAHs have significant absorption bands at longer wavelengths than predicted by the current astronomical models which consider PAHs in their treatment of the radiation balance of the interstellar medium. Two implications are 1)-ionized interstellar PAHs should add weak, broad band structure to the NIR portion of the interstellar extinction curve and 2)- UV poor radiation fields can pump the PAH emission bands provided ionized PAHs are present.

Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery.

PubMed

Li, He; Fan, Xinqi; Chen, Xing

2016-02-01

Light-responsive proteins have been delivered into the cells for controlling intracellular events with high spatial and temporal resolution. However, the choice of wavelength is limited to the UV and visible range; activation of proteins inside the cells using near-infrared (NIR) light, which has better tissue penetration and biocompatibility, remains elusive. Here, we report the development of a single-walled carbon nanotube (SWCNT)-based bifunctional system that enables protein intracellular delivery, followed by NIR activation of the delivered proteins inside the cells. Proteins of interest are conjugated onto SWCNTs via a streptavidin-desthiobiotin (SA-DTB) linkage, where the protein activity is blocked. SWCNTs serve as both a nanocarrier for carrying proteins into the cells and subsequently a NIR sensitizer to photothermally cleave the linkage and release the proteins. The released proteins become active and exert their functions inside the cells. We demonstrated this strategy by intracellular delivery and NIR-triggered nuclear translocation of enhanced green fluorescent protein, and by intracellular delivery and NIR-activation of a therapeutic protein, saporin, in living cells. Furthermore, we showed that proteins conjugated onto SWCNTs via the SA-DTB linkage could be delivered to the tumors, and optically released and activated by using NIR light in living mice.

Tunability and stability of gold nanoparticles obtained from chloroauric acid and sodium thiosulfate reaction

PubMed Central

2012-01-01

In the quest for producing an effective, clinically relevant therapeutic agent, scalability, repeatability, and stability are paramount. In this paper, gold nanoparticles (GNPs) with precisely controlled near-infrared (NIR) absorption are synthesized by a single-step reaction of HAuCl4 and Na2S2O3 without assistance of additional templates, capping reagents, or seeds. The anisotropy in the shape of gold nanoparticles offers high NIR absorption, making it therapeutically relevant. The synthesized products consist of GNPs with different shapes and sizes, including small spherical colloid gold particles and non-spherical gold crystals. The NIR absorption wavelengths and particle size increase with increasing molar ratio of HAuCl4/Na2S2O3. Non-spherical gold particles can be further purified and separated by centrifugation to improve the NIR-absorbing fraction of particles. In-depth studies reveal that GNPs with good structural and optical stability only form in a certain range of the HAuCl4/Na2S2O3 molar ratio, whereas higher molar ratios result in unstable GNPs, which lose their NIR absorption peak due to decomposition and reassembly via Ostwald ripening. Tuning the optical absorption of the gold nanoparticles in the NIR regime via a robust and repeatable method will improve many applications requiring large quantities of desired NIR-absorbing nanoparticles. PMID:22726762

Single-Step Assembly of Multi-Modal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging

PubMed Central

Pinkerton, Nathalie M.; Gindy, Marian E.; Calero-DdelC, Victoria L.; Wolfson, Theodore; Pagels, Robert F.; Adler, Derek; Gao, Dayuan; Li, Shike; Wang, Ruobing; Zevon, Margot; Yao, Nan; Pacheco, Carlos; Therien, Michael J.; Rinaldi, Carlos; Sinko, Patrick J.

2015-01-01

MRI and NIR-active, multi-modal Composite NanoCarriers (CNCs) are prepared using a simple, one-step process, Flash NanoPrecipitation (FNP). The FNP process allows for the independent control of the hydrodynamic diameter, co-core excipient and NIR dye loading, and iron oxide-based nanocrystal (IONC) content of the CNCs. In the controlled precipitation process, 10 nm IONCs are encapsulated into poly(ethylene glycol) stabilized CNCs to make biocompatible T2 contrast agents. By adjusting the formulation, CNC size is tuned between 80 and 360 nm. Holding the CNC size constant at an intensity weighted average diameter of 99 ± 3 nm (PDI width 28 nm), the particle relaxivity varies linearly with encapsulated IONC content ranging from 66 to 533 mM-1s-1 for CNCs formulated with 4 to 16 wt% IONC. To demonstrate the use of CNCs as in vivo MRI contrast agents, CNCs are surface functionalized with liver targeting hydroxyl groups. The CNCs enable the detection of 0.8 mm3 non-small cell lung cancer metastases in mice livers via MRI. Incorporating the hydrophobic, NIR dye PZn3 into CNCs enables complementary visualization with long-wavelength fluorescence at 800 nm. In vivo imaging demonstrates the ability of CNCs to act both as MRI and fluorescent imaging agents. PMID:25925128

Short wavelength infrared optical windows for evaluation of benign and malignant tissues

NASA Astrophysics Data System (ADS)

Sordillo, Diana C.; Sordillo, Laura A.; Sordillo, Peter P.; Shi, Lingyan; Alfano, Robert R.

2017-04-01

There are three short wavelength infrared (SWIR) optical windows outside the conventionally used first near-infrared (NIR) window (650 to 950 nm). They occur in the 1000- to 2500-nm range and may be considered second, third, and fourth NIR windows. The second (1100 to 1350 nm) and third windows (1600 to 1870 nm) are now being explored through label-free linear and multiphoton imaging. The fourth window (2100 to 2350 nm) has been mostly ignored because of water absorption and the absence of sensitive detectors and ultrafast lasers. With the advent of new technology, use of window IV is now possible. Absorption and scattering properties of light through breast and prostate cancer, bone, lipids, and intralipid solutions at these windows were investigated. We found that breast and prostate cancer and bone have longer total attenuation lengths at NIR windows III and IV, whereas fatty tissues and intralipid have longest lengths at windows II and III. Since collagen is the major chromophore at 2100 and 2350 nm, window IV could be especially valuable in evaluating cancers and boney tissues, whereas windows II and III may be more useful for tissues with high lipid content. SWIR windows may be utilized as additional optical tools for the evaluation of collagen in tissues.

Short wavelength infrared optical windows for evaluation of benign and malignant tissues.

PubMed

Sordillo, Diana C; Sordillo, Laura A; Sordillo, Peter P; Shi, Lingyan; Alfano, Robert R

2017-04-01

There are three short wavelength infrared (SWIR) optical windows outside the conventionally used first near-infrared (NIR) window (650 to 950 nm). They occur in the 1000- to 2500-nm range and may be considered second, third, and fourth NIR windows. The second (1100 to 1350 nm) and third windows (1600 to 1870 nm) are now being explored through label-free linear and multiphoton imaging. The fourth window (2100 to 2350 nm) has been mostly ignored because of water absorption and the absence of sensitive detectors and ultrafast lasers. With the advent of new technology, use of window IV is now possible. Absorption and scattering properties of light through breast and prostate cancer, bone, lipids, and intralipid solutions at these windows were investigated. We found that breast and prostate cancer and bone have longer total attenuation lengths at NIR windows III and IV, whereas fatty tissues and intralipid have longest lengths at windows II and III. Since collagen is the major chromophore at 2100 and 2350 nm, window IV could be especially valuable in evaluating cancers and boney tissues, whereas windows II and III may be more useful for tissues with high lipid content. SWIR windows may be utilized as additional optical tools for the evaluation of collagen in tissues.

The Effect of Leaf Stacking on Leaf Reflectance and Vegetation Indices Measured by Contact Probe during the Season

PubMed Central

Neuwirthová, Eva; Lhotáková, Zuzana; Albrechtová, Jana

2017-01-01

The aims of the study were: (i) to compare leaf reflectance in visible (VIS) (400–700 nm), near-infrared (NIR) (740–1140 nm) and short-wave infrared (SWIR) (2000–2400 nm) spectral ranges measured monthly by a contact probe on a single leaf and a stack of five leaves (measurement setup (MS)) of two broadleaved tree species during the vegetative season; and (ii) to test if and how selected vegetation indices differ under these two MS. In VIS, the pigment-related spectral region, the effect of MS on reflectance was negligible. The major influence of MS on reflectance was detected in NIR (up to 25%), the structure-related spectral range; and weaker effect in SWIR, the water-related spectral range. Vegetation indices involving VIS wavelengths were independent of MS while indices combining wavelengths from both VIS and NIR were MS-affected throughout the season. The effect of leaf stacking contributed to weakening the correlation between the leaf chlorophyll content and selected vegetation indices due to a higher leaf mass per area of the leaf sample. The majority of MS-affected indices were better correlated with chlorophyll content in both species in comparison with MS-unaffected indices. Therefore, in terms of monitoring leaf chlorophyll content using the contact probe reflectance measurement, these MS-affected indices should be used with caution, as discussed in the paper. If the vegetation indices are used for assessment of plant physiological status in various times of the vegetative season, then it is essential to take into consideration their possible changes induced by the particular contact probe measurement setup regarding the leaf stacking. PMID:28538685

Comparisons of Spectral Aerosol Single Scattering Albedo in Seoul, South Korea

NASA Technical Reports Server (NTRS)

Mok, Jungbin; Krotkov, Nickolay A.; Torres, Omar; Jethva, Hiren; Loughman, Robert P.; Spinei, Elena; Campanelli, Monica; Li, Zhanqing; Go, Sujung; Labow, Gordon;

Polymer-and glass-based fluorescence standards for the near infrared (NIR) spectral region.

PubMed

Würth, Christian; Hoffmann, Katrin; Behnke, Thomas; Ohnesorge, Marius; Resch-Genger, Ute

2011-05-01

The widespread use and acceptance of fluorescence techniques especially in regulated areas like medical diagnostics is closely linked to standardization concepts that guarantee and improve the comparability and reliability of fluorescence measurements. At the core of such concepts are dependable fluorescence standards that are preferably certified. The ever rising interest in fluorescence measurements in the near-infrared (NIR) spectral region renders the availability of spectral and intensity standards for this wavelength region increasingly important. This encouraged us to develop approaches to solid NIR standards based upon dye-doped polymers and assess their application-relevant properties in comparison to metal ion-doped glasses. The overall goal is here to provide inexpensive, easily fabricated, and robust internal and external calibration tools for a broad variety of fluorescence instruments ranging e.g. from spectrofluorometers over fluorescence microscopes to miniaturized fluorescence sensors. © Springer Science+Business Media, LLC 2010

NIR technology for on-line determination of superficial a(w) and moisture content during the drying process of fermented sausages.

PubMed

Collell, Carles; Gou, Pere; Arnau, Jacint; Muñoz, Israel; Comaposada, Josep

2012-12-01

Three different NIR equipment were evaluated based on their ability to predict superficial water activity (a(w)) and moisture content in two types of fermented sausages (with and without moulds on surface), using partial least squares (PLS) regression models. The instruments differed mainly in wavelength range, resolution and measurement configuration. The most accurate equipment was used in a new experiment to achieve robust models in sausages with different salt contents and submitted to different drying conditions. The models developed showed determination coefficients (R(2)(P)) values of 0.990, 0.910 and 0.984, and RMSEP values of 1.560%, 0.220% and 0.007% for moisture, salt and a(w) respectively. It was demonstrated that NIR spectroscopy could be a suitable non-destructive method for on-line monitoring and control of the drying process in fermented sausages. Copyright © 2012 Elsevier Ltd. All rights reserved.

Near-infrared photoactivatable control of Ca2+ signaling and optogenetic immunomodulation

PubMed Central

He, Lian; Zhang, Yuanwei; Ma, Guolin; Tan, Peng; Li, Zhanjun; Zang, Shengbing; Wu, Xiang; Jing, Ji; Fang, Shaohai; Zhou, Lijuan; Wang, Youjun; Huang, Yun; Hogan, Patrick G; Han, Gang; Zhou, Yubin

2015-01-01

The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded 'photoactivatable adjuvant' to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca2+-modulated activities with tailored function. DOI: http://dx.doi.org/10.7554/eLife.10024.001 PMID:26646180

Research of transmissive near infrared spectroscopy for non-invasive blood glucose measurement

NASA Astrophysics Data System (ADS)

Yang, Wenming; Liao, Ningfang; Li, Yasheng; Shao, Liwei; Huang, Dehuang

2016-10-01

Near infrared (NIR) has prospectively applied in non-invasive blood glucose measurement due to glucose absorption among the 1.0-2.5m spectral bands. However, this significant technology is hard to be developed because of other blood components and low signal-to-noise ratio (SNR). In this work, we presented a non-invasive glucose measurement system using Fourier transform spectrometer which will work in fingertips or other human body tissues. A refrigerated InGaAs detector with high quantum efficiency performing well in the range of 1.0-1.7μm wavelength is used to acquire transmissive radiation. Preliminary experiment investigations were set up to test glucose levels of aqueous solutions with different concentrations. The analytical modeling of the interferogram data is based on arithmetic Fourier transform and supported by the curvilineal characterization. Experimental results show the variation of light intensity among different glucose concentrations and emphasize the obvious absorption of glucose in NIR wave-range. This study confirms the suitability that NIR can be developed in non-invasive glucose measurement.

All-nitride AlxGa1−xN:Mn/GaN distributed Bragg reflectors for the near-infrared

PubMed Central

Capuzzo, Giulia; Kysylychyn, Dmytro; Adhikari, Rajdeep; Li, Tian; Faina, Bogdan; Tarazaga Martín-Luengo, Aitana; Bonanni, Alberta

2017-01-01

Since the technological breakthrough prompted by the inception of light emitting diodes based on III-nitrides, these material systems have emerged as strategic semiconductors not only for the lighting of the future, but also for the new generation of high-power electronic and spintronic devices. While III-nitride optoelectronics in the visible and ultraviolet spectral range is widely established, all-nitride efficient devices in the near-infrared (NIR) are still wanted. Here, through a comprehensive protocol of design, modeling, epitaxial growth and in-depth characterization, we develop AlxGa1−xN:Mn/GaN NIR distributed Bragg reflectors and we show their efficiency in combination with GaN:(Mn,Mg) layers containing Mn-Mgk complexes optically active in the near-infrared range of wavelengths. PMID:28198432

A novel approach to comparing reproductive stage serum profiles in mares using near infrared spectroscopy (NIR) and aquaphotomics

USDA-ARS?s Scientific Manuscript database

The capability of near infrared spectroscopy (NIR) to detect biomolecules in aqueous solutions, a sub-field of NIR called Aquaphotomics, has yet to be fully explored. Aquaphotomics references water absorbance patterns and wavelength shifts in the 1st overtone of the water spectrum as they change pat...

A high-speed, eight-wavelength visible light-infrared pyrometer for shock physics experiments

NASA Astrophysics Data System (ADS)

Wang, Rongbo; Li, Shengfu; Zhou, Weijun; Luo, Zhen-Xiong; Meng, Jianhua; Tian, Jianhua; He, Lihua; Cheng, Xianchao

2017-09-01

An eight-channel, high speed pyrometer for precise temperature measurement is designed and realized in this work. The addition of longer-wavelength channels sensitive at lower temperatures highly expands the measured temperature range, which covers the temperature of interest in shock physics from 1500K-10000K. The working wavelength range is 400-1700nm from visible light to near-infrared (NIR). Semiconductor detectors of Si and InGaAs are used as photoelectric devices, whose bandwidths are 50MHz and 150MHz respectively. Benefitting from the high responsivity and high speed of detectors, the time resolution of the pyrometer can be smaller than 10ns. By combining the high-transmittance beam-splitters and narrow-bandwidth filters, the peak spectrum transmissivity of each channel can be higher than 60%. The gray-body temperatures of NaI crystal under shock-loading are successfully measured by this pyrometer.

In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range

NASA Astrophysics Data System (ADS)

Marchand, Paul J.; Szlag, Daniel; Bouwens, Arno; Lasser, Theo

2018-03-01

Visible light optical coherence tomography has shown great interest in recent years for spectroscopic and high-resolution retinal and cerebral imaging. Here, we present an extended-focus optical coherence microscopy system operating from the visible to the near-infrared wavelength range for high axial and lateral resolution imaging of cortical structures in vivo. The system exploits an ultrabroad illumination spectrum centered in the visible wavelength range (λc = 650 nm, Δλ ˜ 250 nm) offering a submicron axial resolution (˜0.85 μm in water) and an extended-focus configuration providing a high lateral resolution of ˜1.4 μm maintained over ˜150 μm in depth in water. The system's axial and lateral resolution are first characterized using phantoms, and its imaging performance is then demonstrated by imaging the vasculature, myelinated axons, and neuronal cells in the first layers of the somatosensory cortex of mice in vivo.

Nondestructive determination of the modulus of elasticity of Fraxinus mandschurica using near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Yu, Huiling; Liang, Hao; Lin, Xue; Zhang, Yizhuo

2018-04-01

A nondestructive methodology is proposed to determine the modulus of elasticity (MOE) of Fraxinus mandschurica samples by using near-infrared (NIR) spectroscopy. The test data consisted of 150 NIR absorption spectra of the wood samples obtained using an NIR spectrometer, with the wavelength range of 900 to 1900 nm. To eliminate the high-frequency noise and the systematic variations on the baseline, Savitzky-Golay convolution combined with standard normal variate and detrending transformation was applied as data pretreated methods. The uninformative variable elimination (UVE), improved by the evolutionary Monte Carlo (EMC) algorithm and successive projections algorithm (SPA) selected three characteristic variables from full 117 variables. The predictive ability of the models was evaluated concerning the root-mean-square error of prediction (RMSEP) and coefficient of determination (Rp2) in the prediction set. In comparison with the predicted results of all the models established in the experiments, UVE-EMC-SPA-LS-SVM presented the best results with the smallest RMSEP of 0.652 and the highest Rp2 of 0.887. Thus, it is feasible to determine the MOE of F. mandschurica using NIR spectroscopy accurately.

Fluorescence lifetime imaging microscopy using near-infrared contrast agents.

PubMed

Nothdurft, R; Sarder, P; Bloch, S; Culver, J; Achilefu, S

2012-08-01

Although single-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to image molecular processes using a wide range of excitation wavelengths, the captured emission of this technique is confined to the visible spectrum. Here, we explore the feasibility of utilizing near-infrared (NIR) fluorescent molecular probes with emission >700 nm for FLIM of live cells. The confocal microscope is equipped with a 785 nm laser diode, a red-enhanced photomultiplier tube, and a time-correlated single photon counting card. We demonstrate that our system reports the lifetime distributions of NIR fluorescent dyes, cypate and DTTCI, in cells. In cells labelled separately or jointly with these dyes, NIR FLIM successfully distinguishes their lifetimes, providing a method to sort different cell populations. In addition, lifetime distributions of cells co-incubated with these dyes allow estimate of the dyes' relative concentrations in complex cellular microenvironments. With the heightened interest in fluorescence lifetime-based small animal imaging using NIR fluorophores, this technique further serves as a bridge between in vitro spectroscopic characterization of new fluorophore lifetimes and in vivo tissue imaging. © 2012 The Author Journal of Microscopy © 2012 Royal Microscopical Society.

Fluorescence Lifetime Imaging Microscopy Using Near-Infrared Contrast Agents

PubMed Central

Nothdurft, Ralph; Sarder, Pinaki; Bloch, Sharon; Culver, Joseph; Achilefu, Samuel

2013-01-01

Although single-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to image molecular processes using a wide range of excitation wavelengths, the captured emission of this technique is confined to the visible spectrum. Here, we explore the feasibility of utilizing near-infrared (NIR) fluorescent molecular probes with emission >700 nm for FLIM of live cells. The confocal microscope is equipped with a 785 nm laser diode, a red-enhanced photomultiplier tube, and a time-correlated single photon counting card. We demonstrate that our system reports the lifetime distributions of NIR fluorescent dyes, cypate and DTTCI, in cells. In cells labeled separately or jointly with these dyes, NIR FLIM successfully distinguishes their lifetimes, providing a method to sort different cell populations. In addition, lifetime distributions of cells co-incubated with these dyes allow estimate of the dyes’ relative concentrations in complex cellular microenvironments. With the heightened interest in fluorescence lifetime-based small animal imaging using NIR fluorophores, this technique further serves as a bridge between in vitro spectroscopic characterization of new fluorophore lifetimes and in vivo tissue imaging. PMID:22788550

Monitoring Delamination of Thermal Barrier Coatings by Near-Infrared and Upconversion Luminescence Imaging

NASA Technical Reports Server (NTRS)

Eldridge, J. I.; Martin, R. E.; Singh, Jogender; Wolfe, Doug E.

2008-01-01

Previous work has demonstrated that TBC delamination can be monitored by incorporating a thin luminescent sublayer that produces greatly increased luminescence intensity from delaminated regions of the TBC. Initial efforts utilized visible-wavelength luminescence from either europium or erbium doped sublayers. This approach exhibited good sensitivity to delamination of electron-beam physical-vapor-deposited (EB-PVD) TBCs, but limited sensitivity to delamination of the more highly scattering plasma-sprayed TBCs due to stronger optical scattering and to interference by luminescence from rare-earth impurities. These difficulties have now been overcome by new strategies employing near-infrared (NIR) and upconversion luminescence imaging. NIR luminescence at 1550 nm was produced in an erbium plus ytterbium co-doped yttria-stabilized zirconia (YSZ) luminescent sublayer using 980-nm excitation. Compared to visible-wavelength luminescence, these NIR emission and excitation wavelengths are much more weakly scattered by the TBC and therefore show much improved depth-probing capabilities. In addition, two-photon upconversion luminescence excitation at 980 nm wavelength produces luminescence emission at 562 nm with near-zero fluorescence background and exceptional contrast for delamination indication. The ability to detect TBC delamination produced by Rockwell indentation and by furnace cycling is demonstrated for both EB-PVD and plasma-sprayed TBCs. The relative strengths of the NIR and upconversion luminescence methods for monitoring TBC delamination are discussed.

Thermal consequences of colour and near-infrared reflectance.

PubMed

Stuart-Fox, Devi; Newton, Elizabeth; Clusella-Trullas, Susana

2017-07-05

The importance of colour for temperature regulation in animals remains controversial. Colour can affect an animal's temperature because all else being equal, dark surfaces absorb more solar energy than do light surfaces, and that energy is converted into heat. However, in reality, the relationship between colour and thermoregulation is complex and varied because it depends on environmental conditions and the physical properties, behaviour and physiology of the animal. Furthermore, the thermal effects of colour depend as much on absorptance of near-infrared ((NIR), 700-2500 nm) as visible (300-700 nm) wavelengths of direct sunlight; yet the NIR is very rarely considered or measured. The few available data on NIR reflectance in animals indicate that the visible reflectance is often a poor predictor of NIR reflectance. Adaptive variation in animal coloration (visible reflectance) reflects a compromise between multiple competing functions such as camouflage, signalling and thermoregulation. By contrast, adaptive variation in NIR reflectance should primarily reflect thermoregulatory requirements because animal visual systems are generally insensitive to NIR wavelengths. Here, we assess evidence and identify key research questions regarding the thermoregulatory function of animal coloration, and specifically consider evidence for adaptive variation in NIR reflectance.This article is part of the themed issue 'Animal coloration: production, perception, function and application'. © 2017 The Author(s).

NEAR INFRARED DIFFUSE INTERSTELLAR BANDS TOWARD THE CYGNUS OB2 ASSOCIATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamano, Satoshi; Kondo, Sohei; Sameshima, Hiroaki

2016-04-10

We obtained the near-infrared (NIR) high-resolution (R ≡ λ/Δλ ∼ 20,000) spectra of the seven brightest early-type stars in the Cygnus OB2 association for investigating the environmental dependence of diffuse interstellar bands (DIBs). The WINERED spectrograph mounted on the Araki 1.3 m telescope in Japan was used to collect data. All 20 of the known DIBs within the wavelength coverage of WINERED (0.91 < λ < 1.36 μm) were clearly detected along all lines of sight because of their high flux density in the NIR wavelength range and the large extinction. The equivalent widths (EWs) of DIBs were not correlated with the column densities of C{sub 2} molecules,more » which trace the patchy dense component, suggesting that the NIR DIB carriers are distributed mainly in the diffuse component. On the basis of the correlations among the NIR DIBs both for stars in Cyg OB2 and stars observed previously, λλ10780, 10792, 11797, 12623, and 13175 are found to constitute a “family,” in which the DIBs are correlated well over the wide EW range. In contrast, the EW of λ10504 is found to remain almost constant over the stars in Cyg OB2. The extinction estimated from the average EW of λ10504 (A{sub V} ∼ 3.6 mag) roughly corresponds to the lower limit of the extinction distribution of OB stars in Cyg OB2. This suggests that λ10504 is absorbed only by the foreground clouds, implying that the carrier of λ10504 is completely destroyed in Cyg OB2, probably by the strong UV radiation field. The different behaviors of the DIBs may be caused by different properties of the DIB carriers.« less

Estimation of crosstalk in LED fNIRS by photon propagation Monte Carlo simulation

NASA Astrophysics Data System (ADS)

Iwano, Takayuki; Umeyama, Shinji

2015-12-01

fNIRS (functional near-Infrared spectroscopy) can measure brain activity non-invasively and has advantages such as low cost and portability. While the conventional fNIRS has used laser light, LED light fNIRS is recently becoming common in use. Using LED for fNIRS, equipment can be more inexpensive and more portable. LED light, however, has a wider illumination spectrum than laser light, which may change crosstalk between the calculated concentration change of oxygenated and deoxygenated hemoglobins. The crosstalk is caused by difference in light path length in the head tissues depending on wavelengths used. We conducted Monte Carlo simulations of photon propagation in the tissue layers of head (scalp, skull, CSF, gray matter, and white matter) to estimate the light path length in each layers. Based on the estimated path lengths, the crosstalk in fNIRS using LED light was calculated. Our results showed that LED light more increases the crosstalk than laser light does when certain combinations of wavelengths were adopted. Even in such cases, the crosstalk increased by using LED light can be effectively suppressed by replacing the value of extinction coefficients used in the hemoglobin calculation to their weighted average over illumination spectrum.

Near-infrared induced optical quenching effects on mid-infrared quantum cascade lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Dingkai, E-mail: dingk1@umbc.edu; Talukder, Muhammad Anisuzzaman; Chen, Xing

In space communications, atmospheric absorption and Rayleigh scattering are the dominant channel impairments. Transmission using mid-infrared (MIR) wavelengths offers the benefits of lower loss and less scintillation effects. In this work, we report the telecom wavelengths (1.55 μm and 1.3 μm) induced optical quenching effects on MIR quantum cascade lasers (QCLs), when QCLs are operated well above their thresholds. The QCL output power can be near 100% quenched using 20 mW of near-infrared (NIR) power, and the quenching effect depends on the input NIR intensity as well as wavelength. Time resolved measurement was conducted to explore the quenching mechanism. The measured recovery timemore » is around 14 ns, which indicates that NIR generated electron-hole pairs may play a key role in the quenching process. The photocarrier created local field and band bending can effectively deteriorate the dipole transition matrix element and quench the QCL. As a result, MIR QCLs can be used as an optical modulator and switch controlled by NIR lasers. They can also be used as “converters” to convert telecom optical signals into MIR optical signals.« less

Remote sensing of the ocean contributions from ultraviolet to near-infrared using the shortwave infrared bands: simulations.

PubMed

Wang, Menghua

2007-03-20

In the remote sensing of the ocean near-surface properties, it is essential to derive accurate water-leaving radiance spectra through the process of the atmospheric correction. The atmospheric correction algorithm for Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) and Moderate Resolution Imaging Spectroradiometer (MODIS) uses two near-infrared (NIR) bands at 765 and 865 nm (748 and 869 nm for MODIS) for retrieval of aerosol properties with assumption of the black ocean at the NIR wavelengths. Modifications are implemented to account for some of the NIR ocean contributions for the productive but not very turbid waters. For turbid waters in the coastal regions, however, the ocean could have significant contributions in the NIR, leading to significant errors in the satellite-derived ocean water-leaving radiances. For the shortwave infrared (SWIR) wavelengths (approximately > 1000 nm), water has significantly larger absorption than those for the NIR bands. Thus the black ocean assumption at the SWIR bands is generally valid for turbid waters. In addition, for future sensors, it is also useful to include the UV bands to better quantify the ocean organic and inorganic materials, as well as for help in atmospheric correction. Simulations are carried out to evaluate the performance of atmospheric correction for nonabsorbing and weakly absorbing aerosols using the NIR bands and various combinations of the SWIR bands for deriving the water-leaving radiances at the UV (340 nm) and visible wavelengths. Simulations show that atmospheric correction using the SWIR bands can generally produce results comparable to atmospheric correction using the NIR bands. In particular, the water-leaving radiance at the UV band (340 nm) can also be derived accurately. The results from a sensitivity study for the required sensor noise equivalent reflectance, (NE Delta rho), [or the signal-to-noise ratio (SNR)] for the NIR and SWIR bands are provided and discussed.

Near-infrared spectra of the Martian surface: Reading between the lines

NASA Technical Reports Server (NTRS)

Crisp, D.; Bell, J. F., III

1993-01-01

Moderate-resolution near-infrared (NIR) spectra of Mars have been widely used in studies of the Martian surface because many candidate surface materials have distinctive absorption features at these wavelengths. Recent advances in NIR detector technology and instrumentation have also encouraged studies in this spectral region. The use of moderate spectral resolution has often been justified for NIR surface observations because the spectral features produced by most surface materials are relatively broad, and easily discriminated at this resolution. In spite of this, NIR spectra of Mars are usually very difficult to interpret quantitatively. One problem is that NIR surface absorption features are often only a few percent deep, requiring observations with great signal-to-noise ratios. A more significant problem is that gases in the Martian atmosphere contribute numerous absorption features at these wavelengths. Ground-based observers must also contend with variable absorption by several gases in the Earth's atmosphere (H2O, CO2, O3, N2O, CH4, O2). The strong CO2 bands near 1.4, 1.6, 2.0, 2.7, 4.3, and 4.8 micrometers largely preclude the analysis of surface spectral features at these wavelengths. Martian atmospheric water vapor also contributes significant absorption near 1.33, 1.88, and 2.7 micrometers, but water vapor in the Earth's atmosphere poses a much larger problem to ground-based studies of these spectral regions. The third most important NIR absorber in the Martian atmosphere is CO. This gas absorbs most strongly in the relatively-transparent spectral windows near 4.6 and 2.3 micrometers. It also produces 1-10 percent absorption in the solar spectrum at these NIR wavelengths. This solar CO absorption cannot be adequately removed by dividing the Martian spectrum by that of a star, as is commonly done to calibrate ground-based spectroscopic observations, because most stars do not have identical amounts of CO absorption in their spectra. Here, we describe tow effective methods for eliminating contamination of Martian surface spectra by absorption in the solar, terrestrial, and Martian atmospheres. Both methods involve the use of very-high-resolution spectra that completely resolve the narrow atmospheric absorption lines.

GIARPS: the unique VIS-NIR high precision radial velocity facility in this world

NASA Astrophysics Data System (ADS)

Claudi, R.; Benatti, S.; Carleo, I.; Ghedina, A.; Molinari, E.; Oliva, E.; Tozzi, A.; Baruffolo, A.; Cecconi, M.; Cosentino, R.; Fantinel, D.; Fini, L.; Ghinassi, F.; Gonzalez, M.; Gratton, R.; Guerra, J.; Harutyunyan, A.; Hernandez, N.; Iuzzolino, M.; Lodi, M.; Malavolta, L.; Maldonado, J.; Micela, G.; Sanna, N.; Sanjuan, J.; Scuderi, S.; Sozzetti, A.; Pérez Ventura, H.; Diaz Marcos, H.; Galli, A.; Gonzalez, C.; Riverol, L.; Riverol, C.

2016-08-01

GIARPS (GIAno and haRPS) is a project devoted to have on the same focal station of the Telescopio Nazionale Galileo (TNG) both the high resolution spectrographs HARPS-N (VIS) and GIANO (NIR) working simultaneously. This could be considered the first and unique worldwide instrument providing cross-dispersed echelle spectroscopy at a high resolution (R=115,000 in the visual and R=50,000 in the IR) and over in a wide spectral range (0.383 - 2.45 μm) in a single exposure. The science case is very broad, given the versatility of such an instrument and the large wavelength range. A number of outstanding science cases encompassing mainly extra-solar planet science starting from rocky planet search and hot Jupiters, atmosphere characterization can be considered. Furthermore both instrument can measure high precision radial velocity by means the simultaneous thorium technique (HARPS - N) and absorbing cell technique (GIANO) in a single exposure. Other science cases are also possible. Young stars and proto- planetary disks, cool stars and stellar populations, moving minor bodies in the solar system, bursting young stellar objects, cataclysmic variables and X-ray binary transients in our Galaxy, supernovae up to gamma-ray bursts in the very distant and young Universe, can take advantage of the unicity of this facility both in terms of contemporaneous wide wavelength range and high resolution spectroscopy.

Multispectral cross-polarization reflectance measurements suggest high contrast of demineralization on tooth surfaces at wavelengths beyond 1300 nm due to reduced light scattering in sound enamel.

PubMed

Chan, Kenneth H; Fried, Daniel

2018-06-01

The enamel scattering coefficient decreases markedly with increasing wavelength from the visible to the near-infrared (NIR). However, beyond 1300 nm, the scattering coefficient is difficult to measure, and it is not known whether light scattering continues to decrease significantly at longer wavelengths. It is hypothesized that water absorption is a major contributor to the contrast between sound and demineralized enamel beyond 1300 nm since deeply penetrating photons in sound enamel are likely absorbed by water. Reflectance images of demineralization on tooth surfaces were acquired at wavelengths near 1450, 1860, 1880, and 1950 nm. The magnitude of water absorption is similar at 1450 and 1880 nm but varies markedly between 1860, 1880, and 1950 nm. Multispectral comparisons of lesion contrast provide insight into the mechanism responsible for higher contrast at longer NIR wavelengths. The highest contrast was at 1950 nm; however, the markedly higher contrast at 1880 compared to 1450 nm and similar contrast between 1860 and 1880 nm suggests that the enamel scattering coefficient continues to decrease beyond 1300 nm, and that reduced light scattering in sound enamel is most responsible for the higher lesion contrast at longer NIR wavelengths. This has important implications for the choice of wavelengths for caries detection and diagnostic devices, including the performance of optical coherence tomography beyond 1300 nm. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Muscle tissue saturation in humans studied with two non-invasive optical techniques: a comparative study

NASA Astrophysics Data System (ADS)

Shaharin, Alfi; Krite Svanberg, Emilie; Ellerström, Ida; Subash, Arman Ahamed; Khoptyar, Dmitry; Andersson-Engels, Stefan; Åkeson, Jonas

2013-11-01

Muscle tissue saturation (StO2) has been measured with two non-invasive optical techniques and the results were compared. One of the techniques is widely used in the hospitals - the CW-NIRS technique. The other is the photon timeof- flight spectrometer (pTOFS) developed in the Group of Biophotonics, Lund University, Sweden. The wavelengths used in both the techniques are 730 nm and 810 nm. A campaign was arranged to perform measurements on 21 (17 were taken for comparison) healthy adult volunteers (8 women and 13 men). Oxygen saturations were measured at the right lower arm of each volunteer. To observe the effects of different provocations on the oxygen saturation a blood pressure cuff was attached in the upper right arm. For CW-NIRS, the tissue saturation values were in the range from 70-90%, while for pTOFS the values were in the range from 55-60%.

Transmission in near-infrared optical windows for deep brain imaging.

PubMed

Shi, Lingyan; Sordillo, Laura A; Rodríguez-Contreras, Adrián; Alfano, Robert

2016-01-01

Near-infrared (NIR) radiation has been employed using one- and two-photon excitation of fluorescence imaging at wavelengths 650-950 nm (optical window I) for deep brain imaging; however, longer wavelengths in NIR have been overlooked due to a lack of suitable NIR-low band gap semiconductor imaging detectors and/or femtosecond laser sources. This research introduces three new optical windows in NIR and demonstrates their potential for deep brain tissue imaging. The transmittances are measured in rat brain tissue in the second (II, 1,100-1,350 nm), third (III, 1,600-1,870 nm), and fourth (IV, centered at 2,200 nm) NIR optical tissue windows. The relationship between transmission and tissue thickness is measured and compared with the theory. Due to a reduction in scattering and minimal absorption, window III is shown to be the best for deep brain imaging, and windows II and IV show similar but better potential for deep imaging than window I. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

NIR light propagation in a digital head model for traumatic brain injury (TBI)

PubMed Central

Francis, Robert; Khan, Bilal; Alexandrakis, George; Florence, James; MacFarlane, Duncan

2015-01-01

Near infrared spectroscopy (NIRS) is capable of detecting and monitoring acute changes in cerebral blood volume and oxygenation associated with traumatic brain injury (TBI). Wavelength selection, source-detector separation, optode density, and detector sensitivity are key design parameters that determine the imaging depth, chromophore separability, and, ultimately, clinical usefulness of a NIRS instrument. We present simulation results of NIR light propagation in a digital head model as it relates to the ability to detect intracranial hematomas and monitor the peri-hematomal tissue viability. These results inform NIRS instrument design specific to TBI diagnosis and monitoring. PMID:26417498

Simulation and analysis of Au-MgF2 structure in plasmonic sensor in near infrared spectral region

NASA Astrophysics Data System (ADS)

Sharma, Anuj K.

2018-05-01

Plasmonic sensor based on metal-dielectric combination of gold and MgF2 layers is studied in near infrared (NIR) spectral region. An emphasis is given on the effect of variable thickness of MgF2 layer in combination with operating wavelength and gold layer thickness on the sensor's performance in NIR. It is established that the variation in MgF2 thickness in connection with plasmon penetration depth leads to significant variation in sensor's performance. The analysis leads to a conclusion that taking smaller values of MgF2 layer thickness and operating at longer NIR wavelength leads to enhanced sensing performance. Also, fluoride glass can provide better sensing performance than chalcogenide glass and silicon substrate.

A compact multi-wavelength optoacoustic system based on high-power diode lasers for characterization of double-walled carbon nanotubes (DWCNTs) for biomedical applications

NASA Astrophysics Data System (ADS)

Leggio, Luca; de Varona, Omar; Escudero, Pedro; Carpintero del Barrio, Guillermo; Osiński, Marek; Lamela Rivera, Horacio

2015-06-01

During the last decade, Optoacoustic Imaging (OAI), or Optoacoustic Tomography (OAT), has evolved as a novel imaging technique based on the generation of ultrasound waves with laser light. OAI may become a valid alternative to techniques currently used for the detection of diseases at their early stages. It has been shown that OAI combines the high contrast of optical imaging techniques with high spatial resolution of ultrasound systems in deep tissues. In this way, the use of nontoxic biodegradable contrast agents that mark the presence of diseases in near-infrared (NIR) wavelengths range (0.75-1.4 um) has been considered. The presence of carcinomas and harmful microorganisms can be revealed by means of the fluorescence effect exhibited by biopolymer nanoparticles. A different approach is to use carbon nanotubes (CNTs) which are a contrast agent in NIR range due to their absorption characteristics in the range between 800 to 1200 nm. We report a multi-wavelength (870 and 905 nm) laser diode-based optoacoustic (OA) system generating ultrasound signals from a double-walled carbon nanotubes (DWCNTs) solution arranged inside a tissue-like phantom, mimicking the scattering of a biological soft tissue. Optoacoustic signals obtained with DWCNTs inclusions within a tissue-like phantom are compared with the case of ink-filled inclusions, with the aim to assess their absorption. These measurements are done at both 870 and 905 nm, by using high power laser diodes as light sources. The results show that the absorption is relatively high when the inclusion is filled with ink and appreciable with DWCNTs.

Laboratory Simulations and Spectral Analyses of Space Weathering of Non-Ice Materials on Ocean Worlds

NASA Astrophysics Data System (ADS)

Wing, B. R.; Shusterman, M. L.; Irvin, B. L.; Hibbitts, C.

2016-12-01

Airless solar system bodies are subjected to bombardment by high-energy particles from the solar wind and for Galilean satellites, from the Jovian magnetosphere. These keV-MeV electrons and ions damage the upper microns of the exposed surface, resulting in physical, chemical, and spectral alterations that may confound interpretations of mineralogical properties. We conducted experiments simulating space weathering by energetic electrons for characterizing the spectral effects from the UV through the mid-IR; wavelengths commonly used to determine compositions of airless bodies. We bombarded analog non-ice materials with 40 keV electrons under high vacuum conditions for a period of 48-96 hours at a fluence of 80 μA. Spectral measurements were obtained at UV, VIS-SWIR, and NIR-MIR ranges from 0.14-5.0 μm using a McPherson 302 monochrometer, an SVC fiber-fed point spectrometer, and a Bruker Vertex 70 FTIR, respectively. The monochrometer and FTIR measurements were obtained before, during, and after irradiation, while the sample was under vacuum at 1e-7 torr. SVC measurements were obtained in a separate apparatus under an N2-purged environment before and after irradiation. The experiments were conducted to develop a better understanding of how exposure to particulate bombardment may affect the spectral features of airless bodies and subsequent interpretation of composition. Our results characterize the spectral nature of radiation-induced color centers, or Farbe-centers, that are active in the NUV-VIS-NIR wavelength range and inactive in the SWIR-MIR wavelength range. We confirmed the discoloration is due to the formation of F-centers rather than trace contamination such as iron, by analyzing samples under scanning electron microscope and X-ray spectrometer.

Age determination of bottled Chinese rice wine by VIS-NIR spectroscopy

NASA Astrophysics Data System (ADS)

Yu, Haiyan; Lin, Tao; Ying, Yibin; Pan, Xingxiang

2006-10-01

The feasibility of non-invasive visible and near infrared (VIS-NIR) spectroscopy for determining wine age (1, 2, 3, 4, and 5 years) of Chinese rice wine was investigated. Samples of Chinese rice wine were analyzed in 600 mL square brown glass bottles with side length of approximately 64 mm at room temperature. VIS-NIR spectra of 100 bottled Chinese rice wine samples were collected in transmission mode in the wavelength range of 350-1200 nm by a fiber spectrometer system. Discriminant models were developed based on discriminant analysis (DA) together with raw, first and second derivative spectra. The concentration of alcoholic degree, total acid, and °Brix was determined to validate the NIR results. The calibration result for raw spectra was better than that for first and second derivative spectra. The percentage of samples correctly classified for raw spectra was 98%. For 1-, 2-, and 3-year-old sample groups, the sample were all correctly classified, and for 4- and 5-year-old sample groups, the percentage of samples correctly classified was 92.9%, respectively. In validation analysis, the percentage of samples correctly classified was 100%. The results demonstrated that VIS-NIR spectroscopic technique could be used as a non-invasive, rapid and reliable method for predicting wine age of bottled Chinese rice wine.

IR sensitivity enhancement of CMOS Image Sensor with diffractive light trapping pixels.

PubMed

Yokogawa, Sozo; Oshiyama, Itaru; Ikeda, Harumi; Ebiko, Yoshiki; Hirano, Tomoyuki; Saito, Suguru; Oinoue, Takashi; Hagimoto, Yoshiya; Iwamoto, Hayato

2017-06-19

We report on the IR sensitivity enhancement of back-illuminated CMOS Image Sensor (BI-CIS) with 2-dimensional diffractive inverted pyramid array structure (IPA) on crystalline silicon (c-Si) and deep trench isolation (DTI). FDTD simulations of semi-infinite thick c-Si having 2D IPAs on its surface whose pitches over 400 nm shows more than 30% improvement of light absorption at λ = 850 nm and the maximum enhancement of 43% with the 540 nm pitch at the wavelength is confirmed. A prototype BI-CIS sample with pixel size of 1.2 μm square containing 400 nm pitch IPAs shows 80% sensitivity enhancement at λ = 850 nm compared to the reference sample with flat surface. This is due to diffraction with the IPA and total reflection at the pixel boundary. The NIR images taken by the demo camera equip with a C-mount lens show 75% sensitivity enhancement in the λ = 700-1200 nm wavelength range with negligible spatial resolution degradation. Light trapping CIS pixel technology promises to improve NIR sensitivity and appears to be applicable to many different image sensor applications including security camera, personal authentication, and range finding Time-of-Flight camera with IR illuminations.

Determination of Hemicellulose, Cellulose and Lignin in Moso Bamboo by Near Infrared Spectroscopy

PubMed Central

Li, Xiaoli; Sun, Chanjun; Zhou, Binxiong; He, Yong

2015-01-01

The contents of hemicellulose, cellulose and lignin are important for moso bamboo processing in biomass energy industry. The feasibility of using near infrared (NIR) spectroscopy for rapid determination of hemicellulose, cellulose and lignin was investigated in this study. Initially, the linear relationship between bamboo components and their NIR spectroscopy was established. Subsequently, successive projections algorithm (SPA) was used to detect characteristic wavelengths for establishing the convenient models. For hemicellulose, cellulose and lignin, 22, 22 and 20 characteristic wavelengths were obtained, respectively. Nonlinear determination models were subsequently built by an artificial neural network (ANN) and a least-squares support vector machine (LS-SVM) based on characteristic wavelengths. The LS-SVM models for predicting hemicellulose, cellulose and lignin all obtained excellent results with high determination coefficients of 0.921, 0.909 and 0.892 respectively. These results demonstrated that NIR spectroscopy combined with SPA-LS-SVM is a useful, nondestructive tool for the determinations of hemicellulose, cellulose and lignin in moso bamboo. PMID:26601657

A Reversible DNA Logic Gate Platform Operated by One- and Two-Photon Excitations.

PubMed

Tam, Dick Yan; Dai, Ziwen; Chan, Miu Shan; Liu, Ling Sum; Cheung, Man Ching; Bolze, Frederic; Tin, Chung; Lo, Pik Kwan

2016-01-04

We demonstrate the use of two different wavelength ranges of excitation light as inputs to remotely trigger the responses of the self-assembled DNA devices (D-OR). As an important feature of this device, the dependence of the readout fluorescent signals on the two external inputs, UV excitation for 1 min and/or near infrared irradiation (NIR) at 800 nm fs laser pulses, can mimic function of signal communication in OR logic gates. Their operations could be reset easily to its initial state. Furthermore, these DNA devices exhibit efficient cellular uptake, low cytotoxicity, and high bio-stability in different cell lines. They are considered as the first example of a photo-responsive DNA logic gate system, as well as a biocompatible, multi-wavelength excited system in response to UV and NIR. This is an important step to explore the concept of photo-responsive DNA-based systems as versatile tools in DNA computing, display devices, optical communication, and biology. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

SOUL: the Single conjugated adaptive Optics Upgrade for LBT

NASA Astrophysics Data System (ADS)

Pinna, E.; Esposito, S.; Hinz, P.; Agapito, G.; Bonaglia, M.; Puglisi, A.; Xompero, M.; Riccardi, A.; Briguglio, R.; Arcidiacono, C.; Carbonaro, L.; Fini, L.; Montoya, M.; Durney, O.

2016-07-01

We present here SOUL: the Single conjugated adaptive Optics Upgrade for LBT. Soul will upgrade the wavefront sensors replacing the existing CCD detector with an EMCCD camera and the rest of the system in order to enable the closed loop operations at a faster cycle rate and with higher number of slopes. Thanks to reduced noise, higher number of pixel and framerate, we expect a gain (for a given SR) around 1.5-2 magnitudes at all wavelengths in the range 7.5 70% in I-band and 0.6asec seeing) and the sky coverage will be multiplied by a factor 5 at all galactic latitudes. Upgrading the SCAO systems at all the 4 focal stations, SOUL will provide these benefits in 2017 to the LBTI interferometer and in 2018 to the 2 LUCI NIR spectro-imagers. In the same year the SOUL correction will be exploited also by the new generation of LBT instruments: V-SHARK, SHARK-NIR and iLocater.

Contrast-enhanced near-infrared laser mammography with a prototype breast scanner: feasibility study with tissue phantoms and preliminary results of imaging experimental tumors.

PubMed

Boehm, T; Hochmuth, A; Malich, A; Reichenbach, J R; Fleck, M; Kaiser, W A

2001-10-01

Near-infrared (NIR) optical mammography without contrast has a low specificity. The application of optical contrast medium may improve the performance. The concentration-dependent detectability of a new NIR contrast medium was determined with a prototype optical breast scanner. In vivo imaging of experimental tumors was performed. The NIR contrast agent NIR96010 is a newly synthesized, hydrophilic contrast agent for NIR mammography. A concentration-dependent contrast resolution was determined for tissue phantoms consisting of whole milk powder and gelatin. A central part of the phantoms measuring 2 x 2 cm2 without contrast was replaced with phantom material containing 1 micromol/L to 25 nmol/L NIR96010. The composite phantoms were measured with a prototype NIR breast scanner with lasers of lambda1 = 785 nm and lambda2 = 850 nm wavelength. Intensity profiles and standard deviations of the transmission signal in areas with and without contrast were determined by linear fit procedures. Signal-to-noise ratios and spatial resolution as a function of contrast concentration were determined. Near-infrared imaging of five tumor-bearing SCID mice (MX1 breast adenocarcinoma, tumor diameter 5-10 mm) was performed before and after intravenous application of 2 micromol/kg NIR96010. Spectrometry showed an absorption maximum of the contrast agent at 755 nm. No spectral shifts occurred in protein-containing solution. Signal-to-noise ratio in the transmission intensity profiles ranged from 1.1 at 25 nmol/L contrast to 28 at 1 micromol/L. At concentrations <40 nmol/L, no differentiation from the background was possible. The transitional area between the contrast-free edge of the phantom and the central contrast-containing part appeared in the profiles as a steep increase with a width of 4.2 +/- 1.8 mm. The experimental tumors were detectable in nonenhanced images as well as contrast-enhanced images, with better delineation after contrast administration. In postcontrast absorption profiles, a 44.1% +/- 11.3% greater absorption increase was seen in tumor tissue compared with normal tissue. The laser wavelength lambda1 of the prototype laser mammography device was not situated at maximum absorption of the contrast agent NIR96010 but on the descending shoulder of the absorption spectrum. This implies a 20% signal loss for contrast detection. Despite the nonideal measurement conditions, concentrations as low as 40 nmol/L were detectable in vitro. In vivo, all tumors were detectable in color-coded nonenhanced scans as well as in contrast-enhanced scans, with better delineation after contrast administration.

PROXIMA CENTAURI AS A BENCHMARK FOR STELLAR ACTIVITY INDICATORS IN THE NEAR-INFRARED

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robertson, Paul; Bender, Chad; Mahadevan, Suvrath

A new generation of dedicated Doppler spectrographs will attempt to detect low-mass exoplanets around mid- to late M stars at near-infrared (NIR) wavelengths, where those stars are brightest and have the most Doppler information content. A central requirement for the success of these instruments is to properly measure the component of radial velocity (RV) variability contributed by stellar magnetic activity and to account for it in exoplanet models of RV data. The wavelength coverage for many of these new instruments will not include the Ca ii H and K or H α  lines, the most frequently used absorption-line tracers of magneticmore » activity. Thus, it is necessary to define and characterize NIR activity indicators for mid- to late M stars in order to provide simultaneous activity metrics for NIR RV data. We have used the high-cadence UVES observations of the M5.5 dwarf Proxima Centauri from Fuhrmeister et al. to compare the activity sensitivity of eight NIR atomic lines to that of H α . We find that equivalent-width-type measurements of the NIR K i doublet and the Ca ii NIR triplet are excellent proxies for the canonical optical tracers. The Ca ii triplet will be acquired by most of the new and upcoming NIR Doppler spectrographs, offering a common, reliable indicator of activity.« less

Identification of solid state fermentation degree with FT-NIR spectroscopy: Comparison of wavelength variable selection methods of CARS and SCARS.

PubMed

Jiang, Hui; Zhang, Hang; Chen, Quansheng; Mei, Congli; Liu, Guohai

2015-01-01

The use of wavelength variable selection before partial least squares discriminant analysis (PLS-DA) for qualitative identification of solid state fermentation degree by FT-NIR spectroscopy technique was investigated in this study. Two wavelength variable selection methods including competitive adaptive reweighted sampling (CARS) and stability competitive adaptive reweighted sampling (SCARS) were employed to select the important wavelengths. PLS-DA was applied to calibrate identified model using selected wavelength variables by CARS and SCARS for identification of solid state fermentation degree. Experimental results showed that the number of selected wavelength variables by CARS and SCARS were 58 and 47, respectively, from the 1557 original wavelength variables. Compared with the results of full-spectrum PLS-DA, the two wavelength variable selection methods both could enhance the performance of identified models. Meanwhile, compared with CARS-PLS-DA model, the SCARS-PLS-DA model achieved better results with the identification rate of 91.43% in the validation process. The overall results sufficiently demonstrate the PLS-DA model constructed using selected wavelength variables by a proper wavelength variable method can be more accurate identification of solid state fermentation degree. Copyright © 2015 Elsevier B.V. All rights reserved.

Identification of solid state fermentation degree with FT-NIR spectroscopy: Comparison of wavelength variable selection methods of CARS and SCARS

NASA Astrophysics Data System (ADS)

Jiang, Hui; Zhang, Hang; Chen, Quansheng; Mei, Congli; Liu, Guohai

2015-10-01

The use of wavelength variable selection before partial least squares discriminant analysis (PLS-DA) for qualitative identification of solid state fermentation degree by FT-NIR spectroscopy technique was investigated in this study. Two wavelength variable selection methods including competitive adaptive reweighted sampling (CARS) and stability competitive adaptive reweighted sampling (SCARS) were employed to select the important wavelengths. PLS-DA was applied to calibrate identified model using selected wavelength variables by CARS and SCARS for identification of solid state fermentation degree. Experimental results showed that the number of selected wavelength variables by CARS and SCARS were 58 and 47, respectively, from the 1557 original wavelength variables. Compared with the results of full-spectrum PLS-DA, the two wavelength variable selection methods both could enhance the performance of identified models. Meanwhile, compared with CARS-PLS-DA model, the SCARS-PLS-DA model achieved better results with the identification rate of 91.43% in the validation process. The overall results sufficiently demonstrate the PLS-DA model constructed using selected wavelength variables by a proper wavelength variable method can be more accurate identification of solid state fermentation degree.

NIR DLP hyperspectral imaging system for medical applications

NASA Astrophysics Data System (ADS)

Wehner, Eleanor; Thapa, Abhas; Livingston, Edward; Zuzak, Karel

2011-03-01

DLP® hyperspectral reflectance imaging in the visible range has been previously shown to quantify hemoglobin oxygenation in subsurface tissues, 1 mm to 2 mm deep. Extending the spectral range into the near infrared reflects biochemical information from deeper subsurface tissues. Unlike any other illumination method, the digital micro-mirror device, DMD, chip is programmable, allowing the user to actively illuminate with precisely predetermined spectra of illumination with a minimum bandpass of approximately 10 nm. It is possible to construct active spectral-based illumination that includes but is not limited to containing sharp cutoffs to act as filters or forming complex spectra, varying the intensity of light at discrete wavelengths. We have characterized and tested a pure NIR, 760 nm to 1600 nm, DLP hyperspectral reflectance imaging system. In its simplest application, the NIR system can be used to quantify the percentage of water in a subject, enabling edema visualization. It can also be used to map vein structure in a patient in real time. During gall bladder surgery, this system could be invaluable in imaging bile through fatty tissue, aiding surgeons in locating the common bile duct in real time without injecting any contrast agents.

NIR spectrometer using a Schottky photodetector enhanced by grating-based SPR.

PubMed

Chen, Wenjing; Kan, Tetsuo; Ajiki, Yoshiharu; Matsumoto, Kiyoshi; Shimoyama, Isao

2016-10-31

We present a near-infrared (NIR) spectrum measurement method using a Schottky photodetector enhanced by surface plasmon resonance (SPR). An Au grating was fabricated on an n-type silicon wafer to form a Schottky barrier and act as an SPR coupler. The resulting photodetector provides wavelength-selective photodetection depending on the SPR coupling angle. A matrix was pre-calculated to describe this characteristic. The spectrum was obtained from this matrix and the measured photocurrents at various SPR coupling angles. Light with single and multiple wavelengths was tested. Comparative measurements showed that our method is able to detect spectra with a wavelength resolution comparable to that of a commercial spectrometer.

Classification of Fusarium-Infected Korean Hulled Barley Using Near-Infrared Reflectance Spectroscopy and Partial Least Squares Discriminant Analysis

PubMed Central

Lim, Jongguk; Kim, Giyoung; Mo, Changyeun; Oh, Kyoungmin; Yoo, Hyeonchae; Ham, Hyeonheui; Kim, Moon S.

2017-01-01

The purpose of this study is to use near-infrared reflectance (NIR) spectroscopy equipment to nondestructively and rapidly discriminate Fusarium-infected hulled barley. Both normal hulled barley and Fusarium-infected hulled barley were scanned by using a NIR spectrometer with a wavelength range of 1175 to 2170 nm. Multiple mathematical pretreatments were applied to the reflectance spectra obtained for Fusarium discrimination and the multivariate analysis method of partial least squares discriminant analysis (PLS-DA) was used for discriminant prediction. The PLS-DA prediction model developed by applying the second-order derivative pretreatment to the reflectance spectra obtained from the side of hulled barley without crease achieved 100% accuracy in discriminating the normal hulled barley and the Fusarium-infected hulled barley. These results demonstrated the feasibility of rapid discrimination of the Fusarium-infected hulled barley by combining multivariate analysis with the NIR spectroscopic technique, which is utilized as a nondestructive detection method. PMID:28974012

Classification of dry-cured hams according to the maturation time using near infrared spectra and artificial neural networks.

PubMed

Prevolnik, M; Andronikov, D; Žlender, B; Font-i-Furnols, M; Novič, M; Škorjanc, D; Čandek-Potokar, M

2014-01-01

An attempt to classify dry-cured hams according to the maturation time on the basis of near infrared (NIR) spectra was studied. The study comprised 128 samples of biceps femoris (BF) muscle from dry-cured hams matured for 10 (n=32), 12 (n=32), 14 (n=32) or 16 months (n=32). Samples were minced and scanned in the wavelength range from 400 to 2500 nm using spectrometer NIR System model 6500 (Silver Spring, MD, USA). Spectral data were used for i) splitting of samples into the training and test set using 2D Kohonen artificial neural networks (ANN) and for ii) construction of classification models using counter-propagation ANN (CP-ANN). Different models were tested, and the one selected was based on the lowest percentage of misclassified test samples (external validation). Overall correctness of the classification was 79.7%, which demonstrates practical relevance of using NIR spectroscopy and ANN for dry-cured ham processing control. Copyright © 2013 Elsevier Ltd. All rights reserved.

Light-Regulated Polymerization under Near-Infrared/Far-Red Irradiation Catalyzed by Bacteriochlorophyll a.

PubMed

Shanmugam, Sivaprakash; Xu, Jiangtao; Boyer, Cyrille

2016-01-18

Photoregulated polymerizations are typically conducted using high-energy (UV and blue) light, which may lead to undesired side reactions. Furthermore, as the penetration of visible light is rather limited, the range of applications with such wavelengths is likewise limited. We herein report the first living radical polymerization that can be activated and deactivated by irradiation with near-infrared (NIR) and far-red light. Bacteriochlorophyll a (Bachl a) was employed as a photoredox catalyst for photoinduced electron transfer/reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Well-defined polymers were thus synthesized within a few hours under NIR (λ=850 nm) and far-red (λ=780 nm) irradiation with excellent control over the molecular weight (M(n)/M(w)<1.25). Taking advantage of the good penetration of NIR light, we showed that the polymerization also proceeded smoothly when a translucent barrier was placed between light source and reaction vessel. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Novel, near-infrared spectroscopic, label-free, techniques to assess bone abnormalities such as Paget's disease, osteoporosis and bone fractures

NASA Astrophysics Data System (ADS)

Sordillo, Diana C.; Sordillo, Laura A.; Shi, Lingyan; Budansky, Yury; Sordillo, Peter P.; Alfano, Robert R.

2015-02-01

Near- infrared (NIR) light with wavelengths from 650 nm to 950 nm (known as the first NIR window) has conventionally been used as a non-invasive technique that can reach deeper penetration depths through media than light at shorter wavelengths. Recently, several novel, NIR, label-free, techniques have been developed to assess Paget's disease of bone, osteoporosis and bone microfractures. We designed a Bone Optical Analyzer (BOA) which utilizes the first window to measure changes of Hb and HbO2. Paget's disease is marked by an increase in vascularization in bones, and this device can enable easy diagnosis and more frequent monitoring of the patient's condition, without exposing him to a high cumulative dose of radiation. We have also used inverse imaging algorithms to reconstruct 2D and 3D maps of the bone's structure. This device could be used to assess diseases such as osteoporosis. Using 800 nm femtosecond excitation with two-photon (2P) microscopy, we acquired 2PM images of the periosteum and spatial frequency spectra (based on emission of collagen) from the periosteal regions. This technique can provide information on the structure of the periosteum and can detect abnormalities which may be an indication of disease. Most recently, we showed that longer NIR wavelengths in the second and third NIR windows (1100 nm-1350 nm, 1600 nm-1870 nm), could be used to image bone microfractures. Use of NIR light could allow for repeated studies in patients with diseases such as Paget's and osteoporosis quickly and non-invasively, and could impact the current management for these diseases.

A system for simultaneous near-infrared reflectance and transillumination imaging of occlusal carious lesions

NASA Astrophysics Data System (ADS)

Simon, Jacob C.; Darling, Cynthia L.; Fried, Daniel

2016-02-01

Clinicians need technologies to improve the diagnosis of questionable occlusal carious lesions (QOC's) and determine if decay has penetrated to the underlying dentin. Assessing lesion depth from near-infrared (NIR) images holds great potential due to the high transparency of enamel and stain to NIR light at λ=1300-1700-nm, which allows direct visualization and quantified measurements of enamel demineralization. Unfortunately, NIR reflectance measurements alone are limited in utility for approximating occlusal lesion depth >200-μm due to light attenuation from the lesion body. Previous studies sought to combine NIR reflectance and transillumination measurements taken at λ=1300-nm in order to estimate QOC depth and severity. The objective of this study was to quantify the change in lesion contrast and size measured from multispectral NIR reflectance and transillumination images of natural occlusal carious lesions with increasing lesion depth and severity in order to determine the optimal multimodal wavelength combinations for estimating QOC depth. Extracted teeth with varying amounts of natural occlusal decay were measured using a multispectral-multimodal NIR imaging system at prominent wavelengths within the λ=1300-1700-nm spectral region. Image analysis software was used to calculate lesion contrast and area values between sound and carious enamel regions.

Cutaneous porphyrins exhibit anti-stokes fluorescence that is detectable in sebum (Conference Presentation)

NASA Astrophysics Data System (ADS)

Tian, Giselle; Zeng, Haishan; Zhao, Jianhua; Wu, Zhenguo; Al Jasser, Mohammed; Lui, Harvey; Mclean, David I.

2016-02-01

Porphyrins produced by Propionibacterium acnes represent the principal fluorophore associated with acne, and appear as orange-red luminescence under the Wood's lamp. Assessment of acne based on Wood's lamp (UV) or visible light illumination is limited by photon penetration depth and has limited sensitivity for earlier stage lesions. Inducing fluorescence with near infrared (NIR) excitation may provide an alternative way to assess porphyrin-related skin disorders. We discovered that under 785 nm CW laser excitation PpIX powder exhibits fluorescence emission in the shorter wavelength range of 600-715 nm with an intensity that is linearly dependent on the excitation power. We attribute this shorter wavelength emission to anti-Stokes fluorescence. Similar anti-Stokes fluorescence was also detected focally in all skin-derived samples containing porphyrins. Regular (Stokes) fluorescence was present under UV and visible light excitation on ex vivo nasal skin and sebum from uninflamed acne, but not on nose surface smears or sebum from inflamed acne. Co-registered CW laser-excited anti-Stokes fluorescence and fs laser-excited multi-photon fluorescence images of PpIX powder showed similar features. In the skin samples because of the anti-Stokes effect, the NIR-induced fluorescence was presumably specific for porphyrins since there appeared to be no anti-Stokes emission signals from other typical skin fluorophores such as lipids, keratins and collagen. Anti-Stokes fluorescence under NIR CW excitation is more sensitive and specific for porphyrin detection than UV- or visible light-excited regular fluorescence and fs laser-excited multi-photon fluorescence. This approach also has higher image contrast compared to NIR fs laser-based multi-photon fluorescence imaging. The anti-Stokes fluorescence of porphyrins within sebum could potentially be applied to detecting and targeting acne lesions for treatment via fluorescence image guidance.

A polychromator-type near-infrared spectrometer with a high-sensitivity and high-resolution photodiode array detector for pharmaceutical process monitoring on the millisecond time scale.

PubMed

Murayama, Kodai; Genkawa, Takuma; Ishikawa, Daitaro; Komiyama, Makoto; Ozaki, Yukihiro

2013-02-01

In the fine chemicals industry, particularly in the pharmaceutical industry, advanced sensing technologies have recently begun being incorporated into the process line in order to improve safety and quality in accordance with process analytical technology. For estimating the quality of powders without preparation during drug formulation, near-infrared (NIR) spectroscopy has been considered the most promising sensing approach. In this study, we have developed a compact polychromator-type NIR spectrometer equipped with a photodiode (PD) array detector. This detector is consisting of 640 InGaAs-PD elements with 20-μm pitch. Some high-specification spectrometers, which use InGaAs-PD with 512 elements, have a wavelength resolution of about 1.56 nm when covering 900-1700 nm range. On the other hand, the newly developed detector, having the PD with one of the world's highest density, enables wavelength resolution of below 1.25 nm. Moreover, thanks to the combination with a highly integrated charge amplifier array circuit, measurement speed of the detector is higher by two orders than that of existing PD array detectors. The developed spectrometer is small (120 mm × 220 mm × 200 mm) and light (6 kg), and it contains various key devices including the high-density and high-sensitivity PD array detector, NIR technology, and spectroscopy technology for a spectroscopic analyzer that has the required detection mechanism and high sensitivity for powder measurement, as well as a high-speed measuring function for blenders. Moreover, we have evaluated the characteristics of the developed NIR spectrometer, and the measurement of powder samples confirmed that it has high functionality.

Poly(vinylpyrrolidone)-Free Multistep Synthesis of Silver Nanoplates with Plasmon Resonance in the Near Infrared Range.

PubMed

Khan, Assad U; Zhou, Zhengping; Krause, Joseph; Liu, Guoliang

2017-11-01

Herein, a poly(vinylpyrrolidone) (PVP)-free method is described for synthesizing Ag nanoplates that have localized surface plasmon resonance in the near-infrared (NIR) range. Citrate-capped Ag spherical nanoparticles are first grown into small Ag nanoplates that resonate in the range of 500-800 nm. The small Ag nanoplates are used as seeds to further grow into large Ag nanoplates with a lateral dimension of 100-600 nm and a plasmon resonance wavelength of 800-1660 nm and above. The number of growth steps can be increased as desired. Without introducing additional citrate into the solutions of small Ag nanoplate seeds, large Ag nanoplates can be synthesized within minutes. The entire synthesis is completely PVP free, which promotes the nanoparticle growth along the lateral direction to form large Ag nanoplates. The multistep growth and the minimum usage of citrate are essential for the fast growth of high-aspect-ratio Ag nanoplates resonating in the NIR range. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effect of shorter pulse duration in cochlear neural activation with an 810-nm near-infrared laser.

PubMed

Wang, Jingxuan; Tian, Lan; Lu, Jianren; Xia, Ming; Wei, Ying

2017-02-01

Optical neural stimulation in the cochlea has been presented as an alternative technique to the electrical stimulation due to its potential in spatially selectivity enhancement. So far, few studies have selected the near-infrared (NIR) laser in cochlear neural stimulation and limited optical parameter space has been examined. This paper focused on investigating the optical parameter effect on NIR stimulation of auditory neurons, especially under shorter pulse durations. The spiral ganglion neurons in the cochlea of deafened guinea pigs were stimulated with a pulsed 810-nm NIR laser in vivo. The laser radiation was delivered by an optical fiber and irradiated towards the modiolus. Optically evoked auditory brainstem responses (OABRs) with various optical parameters were recorded and investigated. The OABRs could be elicited with the cochlear deafened animals by using the 810-nm laser in a wide pulse duration ranged from 20 to 1000 μs. Results showed that the OABR intensity increased along with the increasing laser radiant exposure of limited range at each specific pulse duration. In addition, for the pulse durations from 20 to 300 μs, the OABR intensity increased monotonically along with the pulse duration broadening. While for pulse durations above 300 μs, the OABR intensity basically kept stable with the increasing pulse duration. The 810-nm NIR laser could be an effective stimulus in evoking the cochlear neuron response. Our experimental data provided evidence to optimize the pulse duration range, and the results suggested that the pulse durations from 20 to 300 μs could be the optimized range in cochlear neural activation with the 810-nm-wavelength laser.

Simulation and performance evaluation of fiber optic sensor for detection of hepatic malignancies in human liver tissues

NASA Astrophysics Data System (ADS)

Sharma, Anuj K.; Gupta, Jyoti; Basu, Rikmantra

2018-01-01

A fiber optic sensor is proposed for the identification of healthy and cancerous liver tissues through determination of their corresponding refractive index values. Existing experimental results describing variation of complex refractive index of liver tissues in near infrared (NIR) spectral region are considered for theoretical calculations. The intensity interrogation method with chalcogenide fiber is considered. The sensor's performance is closely analyzed in terms of its sensitivity at multiple operating wavelengths falling in NIR region. Operating at shorter NIR wavelengths leads to greater sensitivity. The effect of design parameters (sensing region length and fiber core diameter), different launching conditions, and fiber glass materials on sensor's performance is examined. The proposed sensor has the potential to provide high sensitivity of liver tissue detection.

Imaging microfractures and other abnormalities of bone using a supercontinuum laser source with wavelengths in the four NIR optical windows

NASA Astrophysics Data System (ADS)

Sordillo, Laura A.; Sordillo, Peter P.; Budansky, Yury; Leproux, Philippe; Alfano, R. R.

2015-02-01

Many areas of the body such as the tibia have minimal tissue thickness overlying bone. Near-infrared (NIR) optical windows may be used to image more deeply to reveal abnormalities hidden beneath tissue. We report on the potential application of a compact Leukos supercontinuum laser source (model STM-2000-IR) with wavelengths in the four NIR optical windows (from 650 nm to 950 nm, 1,100 nm to 1,350 nm, 1,600 to 1,870, and 2,100 nm to 2,300 nm, respectively) and between 200 - 500 microwatt/nm power, with InGaAs (Goodrich Sensors Inc. SU320- 1.7RT) and InSb detectors (Teledyne Technologies) to image microfractures and abnormalities of bone hidden beneath tissue.

Mercaptosuccinic acid-coated NIR-emitting gold nanoparticles for the sensitive and selective detection of Hg2.

PubMed

Xiong, Xiaodong; Lai, Xiaoqi; Liu, Jinbin

2018-01-05

A sensitive fluorescent detection platform for Hg 2+ was constructed based on mercaptosuccinic acid (MSA) coated near-infrared (NIR)-emitting gold nanoparticles (AuNPs). The thiolated mercaptosuccinic acid was employed as both reducing agent and surface coating ligand in a one-step synthesis of NIR-emitting AuNPs (MSA-AuNPs), which exhibited stable fluorescence with the maximum wavelength at 800nm and a wide range of excitation (220-650nm) with the maxima at 413nm. The MSA coated NIR-emitting AuNPs showed a rapid fluorescence quenching toward Hg 2+ over other metal ions with a limit of detection (LOD, 3δ) as low as 4.8nM. The sensing mechanism investigation revealed that the AuNPs formed aggregation due to the "recognition" of Hg 2+ from the MSA, and the resultant strong coupling interaction between Hg 2+ and Au (I) to further quench the fluorescence of the AuNPs, which synergistically resulted in a highly sensitive and selective fluorescence response toward Hg 2+ . This proposed strategy was also demonstrated the possibility to be used for Hg 2+ detection in water samples. Copyright © 2017. Published by Elsevier B.V.

Mercaptosuccinic acid-coated NIR-emitting gold nanoparticles for the sensitive and selective detection of Hg2 +

NASA Astrophysics Data System (ADS)

Xiong, Xiaodong; Lai, Xiaoqi; Liu, Jinbin

2018-01-01

A sensitive fluorescent detection platform for Hg2 + was constructed based on mercaptosuccinic acid (MSA) coated near-infrared (NIR)-emitting gold nanoparticles (AuNPs). The thiolated mercaptosuccinic acid was employed as both reducing agent and surface coating ligand in a one-step synthesis of NIR-emitting AuNPs (MSA-AuNPs), which exhibited stable fluorescence with the maximum wavelength at 800 nm and a wide range of excitation (220-650 nm) with the maxima at 413 nm. The MSA coated NIR-emitting AuNPs showed a rapid fluorescence quenching toward Hg2 + over other metal ions with a limit of detection (LOD, 3δ) as low as 4.8 nM. The sensing mechanism investigation revealed that the AuNPs formed aggregation due to the "recognition" of Hg2 + from the MSA, and the resultant strong coupling interaction between Hg2 + and Au (I) to further quench the fluorescence of the AuNPs, which synergistically resulted in a highly sensitive and selective fluorescence response toward Hg2 +. This proposed strategy was also demonstrated the possibility to be used for Hg2 + detection in water samples.

A portable, multi-channel fNIRS system for prefrontal cortex: Preliminary study on neurofeedback and imagery tasks (Conference Presentation)

NASA Astrophysics Data System (ADS)

Paik, Seung-ho; Kim, Beop-Min

2016-03-01

fNIRS is a neuroimaging technique which uses near-infrared light source in the 700-1000 nm range and enables to detect hemodynamic changes (i.e., oxygenated hemoglobin, deoxygenated hemoglobin, blood volume) as a response to various brain processes. In this study, we developed a new, portable, prefrontal fNIRS system which has 12 light sources, 15 detectors and 108 channels with a sampling rate of 2 Hz. The wavelengths of light source are 780nm and 850nm. ATxmega128A1, 8bit of Micro controller unit (MCU) with 200~4095 resolution along with MatLab data acquisition algorithm was utilized. We performed a simple left and right finger movement imagery tasks which produced statistically significant changes of oxyhemoglobin concentrations in the dorsolateral prefrontal cortex (dlPFC) areas. We observed that the accuracy of the imagery tasks can be improved by carrying out neurofeedback training, during which a real-time feedback signal is provided to a participating subject. The effects of the neurofeedback training was later visually verified using the 3D NIRfast imaging. Our portable fNIRS system may be useful in non-constraint environment for various clinical diagnoses.

Molecular engineering of a dual emission near-infrared ratiometric fluorophore for the detection of pH at the organism level.

PubMed

Wang, Bo-Lin; Jiang, Chuang; Li, Kun; Liu, Yan-Hong; Xie, Yongmei; Yu, Xiao-Qi

2015-07-07

A near-infrared ratiometric fluorophore (NIR-HBT) was rationally designed and constructed by expanding both the excitation and emission wavelength of the classical ratiometric fluorophore 2-(benzothiazol-2-yl)phenol (HBT) into the near-infrared region. The NIR-HBT was easily synthesized by incorporating the HBT module into the hemicyanine skeleton and showed evident NIR ratiometric fluorophore characteristics. Further application of the new fluorophore for pH detection demonstrated that NIR-HBT possesses superior overall analytical performance and NIR-HBT was successfully applied for detection of acidosis caused by inflammation in living animal tissue, which indicated the potential application value of NIR-HBT in biological imaging and sensing.

Designing the optimal semi-warm NIR spectrograph for SALT via detailed thermal analysis

NASA Astrophysics Data System (ADS)

Wolf, Marsha J.; Sheinis, Andrew I.; Mulligan, Mark P.; Wong, Jeffrey P.; Rogers, Allen

2008-07-01

The near infrared (NIR) upgrade to the Robert Stobie Spectrograph (RSS) on the Southern African Large Telescope (SALT), RSS/NIR, extends the spectral coverage of all modes of the optical spectrograph. The RSS/NIR is a low to medium resolution spectrograph with broadband, spectropolarimetric, and Fabry-Perot imaging capabilities. The optical and NIR arms can be used simultaneously to extend spectral coverage from 3200 Å to approximately 1.6 μm. Both arms utilize high efficiency volume phase holographic gratings via articulating gratings and cameras. The NIR camera incorporates a HAWAII-2RG detector with an Epps optical design consisting of 6 spherical elements and providing subpixel rms image sizes of 7.5 +/- 1.0 μm over all wavelengths and field angles. The NIR spectrograph is semi-warm, sharing a common slit plane and partial collimator with the optical arm. A pre-dewar, cooled to below ambient temperature, houses the final NIR collimator optic, the grating/Fabry-Perot etalon, the polarizing beam splitter, and the first three camera optics. The last three camera elements, blocking filters, and detector are housed in a cryogenically cooled dewar. The semi-warm design concept has long been proposed as an economical way to extend optical instruments into the NIR, however, success has been very limited. A major portion of our design effort entails a detailed thermal analysis using non-sequential ray tracing to interactively guide the mechanical design and determine a truly realizable long wavelength cutoff over which astronomical observations will be sky-limited. In this paper we describe our thermal analysis, design concepts for the staged cooling scheme, and results to be incorporated into the overall mechanical design and baffling.

Photocleavable Hydrogel-Coated Upconverting Nanoparticles: A Multifunctional Theranostic Platform for NIR Imaging and On-Demand Macromolecular Delivery.

PubMed

Jalani, Ghulam; Naccache, Rafik; Rosenzweig, Derek H; Haglund, Lisbet; Vetrone, Fiorenzo; Cerruti, Marta

2016-01-27

Lanthanide-doped upconverting nanoparticles (UCNPs) have emerged as excellent nanotransducers for converting longer wavelength near-infrared (NIR) light to shorter wavelengths spanning the ultraviolet (UV) to the visible (Vis) regions of the spectrum via a multiphoton absorption process, known as upconversion. Here, we report the development of NIR to UV-Vis-NIR UCNPs consisting of LiYF4:Yb(3+)/Tm(3+)@SiO2 individually coated with a 10 ± 2 nm layer of chitosan (CH) hydrogel cross-linked with a photocleavable cross-linker (PhL). We encapsulated fluorescent-bovine serum albumin (FITC-BSA) inside the gel. Under 980 nm excitation, the upconverted UV emission cleaves the PhL cross-links and instantaneously liberates the FITC-BSA under 2 cm thick tissue. The release is immediately arrested if the excitation source is switched off. The upconverted NIR light allows for the tracking of particles under the tissue. Nucleus pulposus (NP) cells cultured with UCNPs are viable both in the presence and in the absence of laser irradiation. Controlled drug delivery of large biomolecules and deep tissue imaging make this system an excellent theranostic platform for tissue engineering, biomapping, and cellular imaging applications.

Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation

NASA Astrophysics Data System (ADS)

Nilsson, A. M. K.; Heinrich, D.; Olajos, J.; Andersson-Engels, S.

1997-10-01

In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy, spectra in a previously unexplored wavelength region 0.8-2.3 μm were recorded from various pig heart tissue samples in vitro: normal myocardium (with and without endo/epicardium), aorta, fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA), revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin, collagen and cholesterol as well as with published data. The results obtained with the NIR spectroscopy technique in terms of its potential to classify different tissue types were compared with those from laser-induced fluorescence (LIF) using 337 nm excitation. LIF and NIR spectroscopy can in combination with PCA be used to discriminate between all previously mentioned tissue groups, apart from fatty versus fibrous tissue (LIF) and aorta versus fibrous tissue (NIR), respectively. The NIR analysis was improved by focusing the PCA to the wavelength segment 2.0-2.3 μm, resulting in successful spectral characterisation of all cardiovascular tissue groups.

Near-infrared transillumination of the maxillary sinuses: overview of methods and preliminary clinical results

NASA Astrophysics Data System (ADS)

Mahmood, Usama; Dehdari, Reza; Cerussi, Albert; Nguyen, Quoc; Kelley, Timothy; Tromberg, Bruce J.; Wong, Brian J.

2005-04-01

Though sinusitis is a significant health problem, it remains a challenging diagnosis for many physicians mainly because of its vague, non-specific symptomology. As such, physicians must often rely on x-rays and CT, which are not only costly but also expose the patient to ionizing radiation. As an alternative to these methods of diagnosis, our laboratory constructed a near infrared (NIR) transillumination system to image the paranasal maxillary sinuses. In contrast to the more conventional form of transillumination, which uses visible light, NIR transillumination uses light with a longer wavelength which is less attenuated by soft tissues, allowing increased signal intensity and tissue penetration. Our NIR transillumination system is low-cost, consisting of a light source containing two series of light emitting diodes, which give off light at wavelengths of 810 nm and 850 nm, and a charge coupled device (CCD) camera sensitive to NIR light. The light source is simply placed in the patient"s mouth and the resultant image created by the transmittance of NIR light is captured with the CCD camera via notebook PC. Using this NIR transillumination system, we imaged the paranasal maxillary sinuses of both healthy patients (n=5) and patients with sinus disease (n=12) and compared the resultant findings with conventional CT scans. We found that air and fluid/tissue-filled spaces can be reasonably distinguished by their differing NIR opacities. Based on these findings, we believe NIR transillumination of the paranasal sinuses may provide a simple, safe, and cost effective modality in the diagnosis and management of sinus disease.

Upregulated epidermal growth factor receptor expression following near-infrared irradiation simulating solar radiation in a three-dimensional reconstructed human corneal epithelial tissue culture model.

PubMed

Tanaka, Yohei; Nakayama, Jun

2016-01-01

Humans are increasingly exposed to near-infrared (NIR) radiation from both natural (eg, solar) and artificial (eg, electrical appliances) sources. Although the biological effects of sun and ultraviolet (UV) exposure have been extensively investigated, the biological effect of NIR radiation is still unclear. We previously reported that NIR as well as UV induces photoaging and standard UV-blocking materials, such as sunglasses, do not sufficiently block NIR. The objective of this study was to investigate changes in gene expression in three-dimensional reconstructed corneal epithelial tissue culture exposed to broad-spectrum NIR irradiation to simulate solar NIR radiation that reaches human tissues. DNA microarray and quantitative real-time polymerase chain reaction analysis were used to assess gene expression levels in a three-dimensional reconstructed corneal epithelial model composed of normal human corneal epithelial cells exposed to water-filtered broad-spectrum NIR irradiation with a contact cooling (20°C). The water-filter allowed 1,000-1,800 nm wavelengths and excluded 1,400-1,500 nm wavelengths. A DNA microarray with >62,000 different probes showed 25 and 150 genes that were up- or downregulated by at least fourfold and twofold, respectively, after NIR irradiation. In particular, epidermal growth factor receptor (EGFR) was upregulated by 19.4-fold relative to control cells. Quantitative real-time polymerase chain reaction analysis revealed that two variants of EGFR in human corneal epithelial tissue were also significantly upregulated after five rounds of 10 J/cm(2) irradiation (P<0.05). We found that NIR irradiation induced the upregulated expression of EGFR in human corneal cells. Since over half of the solar energy reaching the Earth is in the NIR region, which cannot be adequately blocked by eyewear and thus can induce eye damage with intensive or long-term exposure, protection from both UV and NIR radiation may prevent changes in gene expression and in turn eye damage.

Photoacoustic contrast imaging of biological tissues with nanodiamonds fabricated for high near-infrared absorbance

PubMed Central

Zhang, Ti; Cui, Huizhong; Fang, Chia-Yi; Su, Long-Jyun; Ren, Shenqiang; Chang, Huan-Cheng; Yang, Xinmai

2013-01-01

Abstract. Radiation-damaged nanodiamonds (DNDs) are potentially ideal optical contrast agents for photoacoustic (PA) imaging in biological tissues due to their low toxicity and high optical absorbance. PA imaging contrast agents have been limited to quantum dots and gold particles, since most existing carbon-based nanoparticles, including fluorescent nanodiamonds, do not have sufficient optical absorption in the near-infrared (NIR) range. A new DND by He+ ion beam irradiation with very high NIR absorption was synthesized. These DNDs produced a 71-fold higher PA signal on a molar basis than similarly dimensioned gold nanorods, and 7.1 fmol of DNDs injected into rodents could be clearly imaged 3 mm below the skin surface with PA signal enhancement of 567% using an 820-nm laser wavelength. PMID:23400417

Detection of sunn pest-damaged wheat samples using visible/near-infrared spectroscopy based on pattern recognition.

PubMed

Basati, Zahra; Jamshidi, Bahareh; Rasekh, Mansour; Abbaspour-Gilandeh, Yousef

2018-05-30

The presence of sunn pest-damaged grains in wheat mass reduces the quality of flour and bread produced from it. Therefore, it is essential to assess the quality of the samples in collecting and storage centers of wheat and flour mills. In this research, the capability of visible/near-infrared (Vis/NIR) spectroscopy combined with pattern recognition methods was investigated for discrimination of wheat samples with different percentages of sunn pest-damaged. To this end, various samples belonging to five classes (healthy and 5%, 10%, 15% and 20% unhealthy) were analyzed using Vis/NIR spectroscopy (wavelength range of 350-1000 nm) based on both supervised and unsupervised pattern recognition methods. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) as the unsupervised techniques and soft independent modeling of class analogies (SIMCA) and partial least squares-discriminant analysis (PLS-DA) as supervised methods were used. The results showed that Vis/NIR spectra of healthy samples were correctly clustered using both PCA and HCA. Due to the high overlapping between the four unhealthy classes (5%, 10%, 15% and 20%), it was not possible to discriminate all the unhealthy samples in individual classes. However, when considering only the two main categories of healthy and unhealthy, an acceptable degree of separation between the classes can be obtained after classification with supervised pattern recognition methods of SIMCA and PLS-DA. SIMCA based on PCA modeling correctly classified samples in two classes of healthy and unhealthy with classification accuracy of 100%. Moreover, the power of the wavelengths of 839 nm, 918 nm and 995 nm were more than other wavelengths to discriminate two classes of healthy and unhealthy. It was also concluded that PLS-DA provides excellent classification results of healthy and unhealthy samples (R 2  = 0.973 and RMSECV = 0.057). Therefore, Vis/NIR spectroscopy based on pattern recognition techniques can be useful for rapid distinguishing the healthy wheat samples from those damaged by sunn pest in the maintenance and processing centers. Copyright © 2018 Elsevier B.V. All rights reserved.

Tls Field Data Based Intensity Correction for Forest Environments

NASA Astrophysics Data System (ADS)

Heinzel, J.; Huber, M. O.

2016-06-01

Terrestrial laser scanning (TLS) is increasingly used for forestry applications. Besides the three dimensional point coordinates, the 'intensity' of the reflected signal plays an important role in forestry and vegetation studies. The benefit of the signal intensity is caused by the wavelength of the laser that is within the near infrared (NIR) for most scanners. The NIR is highly indicative for various vegetation characteristics. However, the intensity as recorded by most terrestrial scanners is distorted by both external and scanner specific factors. Since details about system internal alteration of the signal are often unknown to the user, model driven approaches are impractical. On the other hand, existing data driven calibration procedures require laborious acquisition of separate reference datasets or areas of homogenous reflection characteristics from the field data. In order to fill this gap, the present study introduces an approach to correct unwanted intensity variations directly from the point cloud of the field data. The focus is on the variation over range and sensor specific distortions. Instead of an absolute calibration of the values, a relative correction within the dataset is sufficient for most forestry applications. Finally, a method similar to time series detrending is presented with the only pre-condition of a relative equal distribution of forest objects and materials over range. Our test data covers 50 terrestrial scans captured with a FARO Focus 3D S120 scanner using a laser wavelength of 905 nm. Practical tests demonstrate that our correction method removes range and scanner based alterations of the intensity.

Dinuclear PhotoCORMs: Dioxygen-Assisted Carbon Monoxide Uncaging from Long-Wavelength-Absorbing Metal-Metal-Bonded Carbonyl Complexes.

PubMed

Li, Zhi; Pierri, Agustin E; Huang, Po-Ju; Wu, Guang; Iretskii, Alexei V; Ford, Peter C

2017-06-05

We describe a new strategy for triggering the photochemical release of caged carbon monoxide (CO) in aerobic media using long-wavelength visible and near-infrared (NIR) light. The dinuclear rhenium-manganese carbonyl complexes (CO) 5 ReMn(CO) 3 (L), where L = phenanthroline (1), bipyridine (2), biquinoline (3), or phenanthrolinecarboxaldehyde (4), each show a strong metal-metal-bond-to-ligand (σ MM → π L *) charge-transfer absorption band at longer wavelengths. Photolysis with deep-red (1 and 2) or NIR (3 and 4) light leads to homolytic cleavage of the Re-Mn bonds to give mononuclear metal radicals. In the absence of trapping agents, these radicals primarily recombine to reform dinuclear complexes. In oxygenated media, however, the radicals react with dioxygen to form species much more labile toward CO release via secondary thermal and/or photochemical reactions. Conjugation of 4, with an amine-terminated poly(ethylene glycol) oligomer, gives a water-soluble derivative with similar photochemistry. In this context, we discuss the potential applications of these dinuclear complexes as visible/NIR-light-photoactivated CO-releasing moieties (photoCORMs).

A Near Infrared Spectroscopy (NIRS) and Chemometric Approach to Improve Apple Fruit Quality Management: A Case Study on the Cultivars "Cripps Pink" and "Braeburn".

PubMed

Eisenstecken, Daniela; Panarese, Alessia; Robatscher, Peter; Huck, Christian W; Zanella, Angelo; Oberhuber, Michael

2015-07-24

The potential of near infrared spectroscopy (NIRS) in the wavelength range of 1000-2500 nm for predicting quality parameters such as total soluble solids (TSS), acidity (TA), firmness, and individual sugars (glucose, fructose, sucrose, and xylose) for two cultivars of apples ("Braeburn" and "Cripps Pink") was studied during the pre- and post-storage periods. Simultaneously, a qualitative investigation on the capability of NIRS to discriminate varieties, harvest dates, storage periods and fruit inhomogeneity was carried out. In order to generate a sample set with high variability within the most relevant apple quality traits, three different harvest time points in combination with five different storage periods were chosen, and the evolution of important quality parameters was followed both with NIRS and wet chemical methods. By applying a principal component analysis (PCA) a differentiation between the two cultivars, freshly harvested vs. long-term stored apples and, notably, between the sun-exposed vs. shaded side of apples could be found. For the determination of quality parameters effective prediction models for titratable acid (TA) and individual sugars such as fructose, glucose and sucrose by using partial least square (PLS) regression have been developed. Our results complement earlier reports, highlighting the versatility of NIRS as a fast, non-invasive method for quantitative and qualitative studies on apples.

Near-infrared fluorescence imaging with a mobile phone (Conference Presentation)

NASA Astrophysics Data System (ADS)

Ghassemi, Pejhman; Wang, Bohan; Wang, Jianting; Wang, Quanzeng; Chen, Yu; Pfefer, T. Joshua

2017-03-01

Mobile phone cameras employ sensors with near-infrared (NIR) sensitivity, yet this capability has not been exploited for biomedical purposes. Removing the IR-blocking filter from a phone-based camera opens the door to a wide range of techniques and applications for inexpensive, point-of-care biophotonic imaging and sensing. This study provides proof of principle for one of these modalities - phone-based NIR fluorescence imaging. An imaging system was assembled using a 780 nm light source along with excitation and emission filters with 800 nm and 825 nm cut-off wavelengths, respectively. Indocyanine green (ICG) was used as an NIR fluorescence contrast agent in an ex vivo rodent model, a resolution test target and a 3D-printed, tissue-simulating vascular phantom. Raw and processed images for red, green and blue pixel channels were analyzed for quantitative evaluation of fundamental performance characteristics including spectral sensitivity, detection linearity and spatial resolution. Mobile phone results were compared with a scientific CCD. The spatial resolution of CCD system was consistently superior to the phone, and green phone camera pixels showed better resolution than blue or green channels. The CCD exhibited similar sensitivity as processed red and blue pixels channels, yet a greater degree of detection linearity. Raw phone pixel data showed lower sensitivity but greater linearity than processed data. Overall, both qualitative and quantitative results provided strong evidence of the potential of phone-based NIR imaging, which may lead to a wide range of applications from cancer detection to glucose sensing.

Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells

PubMed Central

Kong, Xiangduo; Mohanty, Samarendra K.; Stephens, Jared; Heale, Jason T.; Gomez-Godinez, Veronica; Shi, Linda Z.; Kim, Jong-Soo; Yokomori, Kyoko; Berns, Michael W.

2009-01-01

Proper recognition and repair of DNA damage is critical for the cell to protect its genomic integrity. Laser microirradiation ranging in wavelength from ultraviolet A (UVA) to near-infrared (NIR) can be used to induce damage in a defined region in the cell nucleus, representing an innovative technology to effectively analyze the in vivo DNA double-strand break (DSB) damage recognition process in mammalian cells. However, the damage-inducing characteristics of the different laser systems have not been fully investigated. Here we compare the nanosecond nitrogen 337 nm UVA laser with and without bromodeoxyuridine (BrdU), the nanosecond and picosecond 532 nm green second-harmonic Nd:YAG, and the femtosecond NIR 800 nm Ti:sapphire laser with regard to the type(s) of damage and corresponding cellular responses. Crosslinking damage (without significant nucleotide excision repair factor recruitment) and single-strand breaks (with corresponding repair factor recruitment) were common among all three wavelengths. Interestingly, UVA without BrdU uniquely produced base damage and aberrant DSB responses. Furthermore, the total energy required for the threshold H2AX phosphorylation induction was found to vary between the individual laser systems. The results indicate the involvement of different damage mechanisms dictated by wavelength and pulse duration. The advantages and disadvantages of each system are discussed. PMID:19357094

Design and experiment of spectrometer based on scanning micro-grating integrating with angle sensor

NASA Astrophysics Data System (ADS)

Biao, Luo; Wen, Zhi-yu

2014-01-01

A compact, low cost, high speed, non-destructive testing NIR (near infrared) spectrometer optical system based on MOEMS grating device is developed. The MOEMS grating works as the prismatic element and wavelength scanning element in our optical system. The MOEMS grating enables the design of compact grating spectrometers capable of acquiring full spectra using a single detector element. This MOEMS grating is driven by electromagnetic force and integrated with angle sensor which used to monitored deflection angle while the grating working. Comparing with the traditional spectral system, there is a new structure with a single detector and worked at high frequency. With the characteristics of MOEMS grating, the structure of the spectrometer system is proposed. After calculating the parameters of the optical path, ZEMAX optical software is used to simulate the system. According the ZEMAX output file of the 3D model, the prototype is designed by SolidWorks rapidly, fabricated. Designed for a wavelength range between 800 nm and 1500 nm, the spectrometer optical system features a spectral resolution of 16 nm with the volume of 97 mm × 81.7 mm × 81 mm. For the purpose of reduce modulated effect of sinusoidal rotation, spectral intensity of the different wavelength should be compensated by software method in the further. The system satisfies the demand of NIR micro-spectrometer with a single detector.

Photoluminescence enhancement of silicon quantum dot monolayer by plasmonic substrate fabricated by nano-imprint lithography

NASA Astrophysics Data System (ADS)

Yanagawa, Hiroto; Inoue, Asuka; Sugimoto, Hiroshi; Shioi, Masahiko; Fujii, Minoru

2017-12-01

Near-field coupling between a silicon quantum dot (Si-QD) monolayer and a plasmonic substrate fabricated by nano-imprint lithography and having broad multiple resonances in the near-infrared (NIR) window of biological substances was studied by precisely controlling the QDs-substrate distance. A strong enhancement of the NIR photoluminescence (PL) of Si-QDs was observed. Detailed analyses of the PL and PL excitation spectra, the PL decay dynamics, and the reflectance spectra revealed that both the excitation cross-sections and the emission rates are enhanced by the surface plasmon resonances, thanks to the broad multiple resonances of the plasmonic substrate, and that the relative contribution of the two enhancement processes depends strongly on the excitation wavelength. Under excitation by short wavelength photons (405 nm), where enhancement of the excitation cross-section is not expected, the maximum enhancement was obtained when the QDs-substrate distance was around 30 nm. On the other hand, under long wavelength excitation (641 nm), where strong excitation cross-section enhancement is expected, the largest enhancement was obtained when the distance was minimum (around 1 nm). The achievement of efficient excitation of NIR luminescence of Si-QDs by long wavelength photons paves the way for the development of Si-QD-based fluorescence bio-sensing devices with a high bound-to-free ratio.

Spectroscopic photoacoustics for assessing ischemic kidney damage

NASA Astrophysics Data System (ADS)

Berndl, Elizabeth S. L.; He, Xiaolin; Yuen, Darren A.; Kolios, Michael C.

2018-02-01

Ischemic reperfusion injuries (IRIs) are caused by return of blood to a tissue or organ after a period without oxygen or nutrients. Damage in the microvasculature causes an inflammatory response and heterogeneous scarring, which is associated with an increase in collagen in the extracellular matrix. Although most often associated with heart attacks and strokes, IRI also occurs when blood reperfuses a transplanted organ. Currently, monitoring for IRI is limited to biopsies, which are invasive and sample a limited area. In this work, we explored photoacoustic (PA) biomarkers of scarring. IRI events were induced in mice (n=2) by clamping the left renal artery, then re-establishing flow. At 53 days post-surgery, kidneys were saline perfused and cut in half laterally. One half was immediately imaged with a VevoX system (Fujifilm-VisualSonics, Toronto) in two near infrared ranges - 680 to 970 nm (NIR), and 1200 to 1350 nm (NIR II). The other half was decellularized and then imaged at NIR and NIR II. Regions of interest were manually identified and analyzed for each kidney. For both cellularized and decellularized samples, the PA signal ratio based on irradiation wavelengths of 715:930 nm was higher in damaged kidneys than for undamaged kidneys (p < 0.0001 for both). Damaged kidneys had ROIs with spectra indicating the presence of collagen in the NIR II range, while healthy kidneys did not. Collagen rich spectra were more apparent in decellularized kidneys, suggesting that in the cellularized samples, other components may be contributing to the signal. PA imaging using spectral ratios associated with collagen signatures may provide a non-invasive tool to determine areas of tissue damage due to IRIs.

Optical wavelength selection for portable hemoglobin determination by near-infrared spectroscopy method

NASA Astrophysics Data System (ADS)

Tian, Han; Li, Ming; Wang, Yue; Sheng, Dinggao; Liu, Jun; Zhang, Linna

2017-11-01

Hemoglobin concentration is commonly used in clinical medicine to diagnose anemia, identify bleeding, and manage red blood cell transfusions. The golden standard method for determining hemoglobin concentration in blood requires reagent. Spectral methods were advantageous at fast and non-reagent measurement. However, model calibration with full spectrum is time-consuming. Moreover, it is necessary to use a few variables considering size and cost of instrumentation, especially for a portable biomedical instrument. This study presents different wavelength selection methods for optical wavelengths for total hemoglobin concentration determination in whole blood. The results showed that modelling using only two wavelengths combination (1143 nm, 1298 nm) can keep on the fine predictability with full spectrum. It appears that the proper selection of optical wavelengths can be more effective than using the whole spectra for determination hemoglobin in whole blood. We also discussed the influence of water absorptivity on the wavelength selection. This research provides valuable references for designing portable NIR instruments determining hemoglobin concentration, and may provide some experience for noninvasive hemoglobin measurement by NIR methods.

Performance of PHOTONIS' low light level CMOS imaging sensor for long range observation

NASA Astrophysics Data System (ADS)

Bourree, Loig E.

2014-05-01

Identification of potential threats in low-light conditions through imaging is commonly achieved through closed-circuit television (CCTV) and surveillance cameras by combining the extended near infrared (NIR) response (800-10000nm wavelengths) of the imaging sensor with NIR LED or laser illuminators. Consequently, camera systems typically used for purposes of long-range observation often require high-power lasers in order to generate sufficient photons on targets to acquire detailed images at night. While these systems may adequately identify targets at long-range, the NIR illumination needed to achieve such functionality can easily be detected and therefore may not be suitable for covert applications. In order to reduce dependency on supplemental illumination in low-light conditions, the frame rate of the imaging sensors may be reduced to increase the photon integration time and thus improve the signal to noise ratio of the image. However, this may hinder the camera's ability to image moving objects with high fidelity. In order to address these particular drawbacks, PHOTONIS has developed a CMOS imaging sensor (CIS) with a pixel architecture and geometry designed specifically to overcome these issues in low-light level imaging. By combining this CIS with field programmable gate array (FPGA)-based image processing electronics, PHOTONIS has achieved low-read noise imaging with enhanced signal-to-noise ratio at quarter moon illumination, all at standard video frame rates. The performance of this CIS is discussed herein and compared to other commercially available CMOS and CCD for long-range observation applications.

Hyperspectral Reflectance Imaging Technique for Visualization of Moisture Distribution in Cooked Chicken Breast

PubMed Central

Kandpal, Lalit Mohan; Lee, Hoonsoo; Kim, Moon S.; Mo, Changyeun; Cho, Byoung-Kwan

2013-01-01

Spectroscopy has proven to be an efficient tool for measuring the properties of meat. In this article, hyperspectral imaging (HSI) techniques are used to determine the moisture content in cooked chicken breast over the VIS/NIR (400–1,000 nm) spectral range. Moisture measurements were performed using an oven drying method. A partial least squares regression (PLSR) model was developed to extract a relationship between the HSI spectra and the moisture content. In the full wavelength range, the PLSR model possessed a maximum R2p of 0.90 and an SEP of 0.74%. For the NIR range, the PLSR model yielded an R2p of 0.94 and an SEP of 0.71%. The majority of the absorption peaks occurred around 760 and 970 nm, representing the water content in the samples. Finally, PLSR images were constructed to visualize the dehydration and water distribution within different sample regions. The high correlation coefficient and low prediction error from the PLSR analysis validates that HSI is an effective tool for visualizing the chemical properties of meat. PMID:24084119

Building Computer Free Sorting Devices Based on Reflection of Visible and NIR Wavelengths

USDA-ARS?s Scientific Manuscript database

NIR and visible light reflection from food products has long been the basis of scientific research for the detection of defects and contaminants as well as food quality attributes. Most of the research in this area reports derived calibration equations that indicate the potential of using the techno...

Crystal phase-controlled synthesis of rod-shaped AgInTe2 nanocrystals for in vivo imaging in the near-infrared wavelength region

NASA Astrophysics Data System (ADS)

Kameyama, Tatsuya; Ishigami, Yujiro; Yukawa, Hiroshi; Shimada, Taisuke; Baba, Yoshinobu; Ishikawa, Tetsuya; Kuwabata, Susumu; Torimoto, Tsukasa

2016-03-01

Rod-shaped AgInTe2 nanocrystals (NCs) exhibiting intense near-band edge photoluminescence in the near-infrared (NIR) wavelength region, were successfully prepared by the thermal reaction of metal acetates and Te precursors in 1-dodecanethiol. Increasing the reaction temperature resulted in the formation of larger AgInTe2 NCs with crystal structures varying from hexagonal to tetragonal at reaction temperatures of 280 °C or higher. The energy gap was increased from 1.13 to 1.20 eV with a decrease in rod width from 8.3 to 5.6 nm, accompanied by a blue shift in the photoluminescence (PL) peak wavelength from 1097 to 1033 nm. The optimal PL quantum yield was approximately 18% for AgInTe2 NCs with rod widths of 5.6 nm. The applicability of AgInTe2 NCs as a NIR-emitting material for in vivo biological imaging was examined by injecting AgInTe2 NC-incorporated liposomes into the back of a C57BL/6 mouse, followed by in vivo photoluminescence imaging in the NIR region.Rod-shaped AgInTe2 nanocrystals (NCs) exhibiting intense near-band edge photoluminescence in the near-infrared (NIR) wavelength region, were successfully prepared by the thermal reaction of metal acetates and Te precursors in 1-dodecanethiol. Increasing the reaction temperature resulted in the formation of larger AgInTe2 NCs with crystal structures varying from hexagonal to tetragonal at reaction temperatures of 280 °C or higher. The energy gap was increased from 1.13 to 1.20 eV with a decrease in rod width from 8.3 to 5.6 nm, accompanied by a blue shift in the photoluminescence (PL) peak wavelength from 1097 to 1033 nm. The optimal PL quantum yield was approximately 18% for AgInTe2 NCs with rod widths of 5.6 nm. The applicability of AgInTe2 NCs as a NIR-emitting material for in vivo biological imaging was examined by injecting AgInTe2 NC-incorporated liposomes into the back of a C57BL/6 mouse, followed by in vivo photoluminescence imaging in the NIR region. Electronic supplementary information (ESI) available: A detailed synthesis procedure of DSPC-AgInTe2 and analytical data of AgInTe2 NCs. See DOI: 10.1039/c5nr07532g

Deep tissue imaging of microfracture and non-displaced fracture of bone using the second and third near-infrared therapeutic windows

NASA Astrophysics Data System (ADS)

Sordillo, Laura A.; Pu, Yang; Sordillo, P. P.; Budansky, Yury; Alfano, Robert R.

2014-03-01

Near-infrared (NIR) light in the wavelengths of 700 nm to 2,000 nm has three NIR optical, or therapeutic, windows, which allow for deeper depth penetration in scattering tissue media. Microfractures secondary to repetitive stress, particularly in the lower extremities, are an important problem for military recruits and athletes. They also frequently occur in the elderly, or in patients taking bisphosphonates or denosumab. Microfractures can be early predictors of a major bone fracture. Using the second and third NIR therapeutic windows, we investigated the results from images of chicken bone and human tibial bone with microfractures and non-displaced fractures with and without overlying tissues of various thicknesses. Images of bone with microfractures and non-displaced fractures with tissue show scattering photons in the third NIR window with wavelengths between 1,650 nm and 1,870 nm are diminished and absorption is increased slightly from and second NIR windows. Results from images of fractured bones show the attenuation length of light through tissue in the third optical window to be larger than in the second therapeutic window. Use of these windows may aid in the detection of bone microfractures, and thus reduce the incidence of major bone fracture in susceptible groups.

Firmness prediction in Prunus persica 'Calrico' peaches by visible/short-wave near infrared spectroscopy and acoustic measurements using optimised linear and non-linear chemometric models.

PubMed

Lafuente, Victoria; Herrera, Luis J; Pérez, María del Mar; Val, Jesús; Negueruela, Ignacio

2015-08-15

In this work, near infrared spectroscopy (NIR) and an acoustic measure (AWETA) (two non-destructive methods) were applied in Prunus persica fruit 'Calrico' (n = 260) to predict Magness-Taylor (MT) firmness. Separate and combined use of these measures was evaluated and compared using partial least squares (PLS) and least squares support vector machine (LS-SVM) regression methods. Also, a mutual-information-based variable selection method, seeking to find the most significant variables to produce optimal accuracy of the regression models, was applied to a joint set of variables (NIR wavelengths and AWETA measure). The newly proposed combined NIR-AWETA model gave good values of the determination coefficient (R(2)) for PLS and LS-SVM methods (0.77 and 0.78, respectively), improving the reliability of MT firmness prediction in comparison with separate NIR and AWETA predictions. The three variables selected by the variable selection method (AWETA measure plus NIR wavelengths 675 and 697 nm) achieved R(2) values 0.76 and 0.77, PLS and LS-SVM. These results indicated that the proposed mutual-information-based variable selection algorithm was a powerful tool for the selection of the most relevant variables. © 2014 Society of Chemical Industry.

Prediction of Caffeine Content in Java Preanger Coffee Beans by NIR Spectroscopy Using PLS and MLR Method

NASA Astrophysics Data System (ADS)

Budiastra, I. W.; Sutrisno; Widyotomo, S.; Ayu, P. C.

2018-05-01

Caffeine is one of important components in coffee that contributes to the coffee beverages flavor. Caffeine concentration in coffee bean is usually determined by chemical method which is time consuming and destructive method. A nondestructive method using NIR spectroscopy was successfully applied to determine the caffeine concentration of Arabica gayo coffee bean. In this study, NIR Spectroscopy was assessed to determine the caffeine concentration of java preanger coffee bean. A hundred samples, each consist of 96 g coffee beans were prepared for reflectance and chemical measurement. Reflectance of the sample was measured by FT-NIR spectrometer in the wavelength of 1000-2500 nm (10000-4000 cm-1) followed by determination of caffeine content using LCMS method. Calibration of NIR spectra and the caffeine content was carried out using PLS and MLR methods. Several spectra data processing was conducted to increase the accuracy of prediction. The result of the study showed that caffeine content could be determined by PLS model using 7 factors and spectra data processing of combination of the first derivative and MSC of spectra absorbance (r = 0.946; CV = 1.54 %; RPD = 2.28). A lower accuracy was obtained by MLR model consisted of three caffeine and other four absorption wavelengths (r = 0.683; CV = 3.31%; RPD = 1.18).

Single bead near-infrared random laser based on silica-gel infiltrated with Rhodamine 640

NASA Astrophysics Data System (ADS)

Moura, André L.; Barbosa-Silva, Renato; Dominguez, Christian T.; Pecoraro, Édison; Gomes, Anderson S. L.; de Araújo, Cid B.

2018-04-01

Photoluminescence properties of single bead silica-gel (SG) embedded with a laser-dye were studied aiming at the operation of near-infrared (NIR) Random Lasers (RLs). The operation of RLs in the NIR spectral region is especially important for biological applications since the optical radiation has deep tissue penetration with negligible damage. Since laser-dyes operating in the NIR have poor stability and are poor emitters, ethanol solutions of Rhodamine 640 (Rh640) infiltrated in SG beads were used. The Rh640 concentrations in ethanol varied from 10-5 to 10-2 M and the excitation at 532 nm was made by using a 7 ns pulsed laser. The proof-of-principle RL scheme herein presented was adopted in order to protect the dye-molecules from the environment and to favor formation of aggregates. The RL emission from ≈650 nm to 720 nm, beyond the typical Rh640 monomer and dimer wavelengths emissions range, was attributed to the trade-off between reabsorption and reemission processes along the light pathways inside the SG bead and the contribution of Rh640 aggregates.

Ultrasensitive near-infrared photodetectors based on graphene-MoTe2-graphene vertical van der Waals heterostructure

NASA Astrophysics Data System (ADS)

Zhang, Kun; Ye, Yu; Dai, Lun; School of Physics, Peking University Team

Two-dimensional (2D) materials have rapidly established themselves as exceptional building blocks for optoelectronic applications, due to their unique properties and atomically thin nature. Nevertheless, near-infrared (NIR) photodetectors based on layered 2D semiconductors are rarely realized. In this work, we fabricate graphene-MoTe2-graphene vertical vdWs heterostructure by a facile and reliable site controllable transfer method, and apply it for photodetection from visible to the NIR wavelength range. Compared to the 2D semiconductor based photodetectors reported thus far, the graphene-MoTe2-graphene photodetector has superior performance, including high photoresponsivity (110 mA W-1 at 1064 nm and 205 mA W-1 at 473 nm), high external quantum efficiency (EQE, 12.9% at 1064 nm and 53.8% at 473 nm), rapid response and recovery processes (rise time of 24 μs, fall time of 46 μs under 1064 nm illumination), and free from an external source-drain power supply. The all-2D-materials heterostructure has promising applications in future novel high responsivity, high speed and flexible NIR devices.

Design of near-infrared dyes for nonlinear optics: toward optical limiting applications at telecommunication wavelengths

NASA Astrophysics Data System (ADS)

Bellier, Quentin; Bouit, Pierre-Antoine; Kamada, Kenji; Feneyrou, Patrick; Malmström, E.; Maury, Olivier; Andraud, Chantal

2009-09-01

The rapid development of frequency-tunable pulsed lasers up to telecommunication wavelengths (1400-1600 nm) led to the design of new materials for nonlinear absorption in this spectral range. In this context, two families of near infra-red (NIR) chromophores, namely heptamethine cyanine and aza-borondipyrromethene (aza-bodipy) dyes were studied. In both cases, they show significant two-photon absorption (TPA) cross-sections in the 1400-1600 nm spectral range and display good optical power limiting (OPL) properties. OPL curves were interpreted on the basis of TPA followed by excited state absorption (ESA) phenomena. Finally these systems have several relevant properties like nonlinear absorption properties, gram scale synthesis and high solubility. In addition, they could be functionalized on several sites which open the way to numerous practical applications in biology, solid-state optical limiting and signal processing.

Broad-band and polarization-independent perfect absorption in graphene-gold cylinder arrays at visible and near-infrared wavelengths

NASA Astrophysics Data System (ADS)

Zhou, P.; Zheng, G. G.; Xu, L. H.; Xian, F. L.; Lai, M.

2018-07-01

A wavelength tunable perfect absorber with graphene-hexagonal gold (Au) cylinder array on a ground plate is investigated theoretically. The interactions between electromagnetic (EM) waves and monolayer graphene are analyzed through the field distributions and spectral responses in detail. The finite-difference-time-domain (FDTD) method is used to investigate the tunable properties of the absorber. It is demonstrated that in an optimized configuration, monolayer graphene can interact with light via critical coupling, and the absorptance can be greatly enhanced and reach to 100% for both transverse magnetic (TM) and transverse electronic (TE) polarizations. Furthermore, the influence of geometrical parameters of the structure on the response of the hybrid structure is studied. It is expected that the proposed graphene perfect absorbers can be applied for many applications in the visible (VIS) and the near-infrared (NIR) spectral ranges such as wavelength selective infrared photodetectors and plasmonic sensors.

Development of online NIR urine analyzing system based on AOTF

NASA Astrophysics Data System (ADS)

Wan, Feng; Sun, Zhendong; Li, Xiaoxia

2006-09-01

In this paper, some key techniques on development of on-line MR urine analyzing system based on AOTF (Acousto - Optics Tunable Filter) are introduced. Problems about designing the optical system including collimation of incident light and working distance (the shortest distance for separating incident light and diffracted light) are analyzed and researched. DDS (Direct Digital Synthesizer) controlled by microprocessor is used to realize the wavelength scan. The experiment results show that this MR urine analyzing system based on. AOTF has 10000 - 4000cm -1 wavelength range and O.3ms wavelength transfer rate. Compare with the conventional Fourier Transform NIP. spectrophotometer for analyzing multi-components in urine, this system features low cost, small volume and on-line measurement function. Unscrambler software (multivariate statistical software by CAMO Inc. Norway) is selected as the software for processing the data. This system can realize on line quantitative analysis of protein, urea and creatinine in urine.

Elucidation of the role of biological factors and device design in cerebral NIRS using an in vivo hematoma model based on high-intensity focused ultrasound

NASA Astrophysics Data System (ADS)

Wang, Jianting; Huang, Stanley; Myers, Matthew; Chen, Yu; Welle, Cristin; Pfefer, Joshua

2016-03-01

Near-Infrared Spectroscopy (NIRS) is an emerging medical countermeasure for rapid, field detection of hematomas caused by traumatic brain injury (TBI). Bench and animal tests to determine NIRS sensitivity and specificity are needed. However, current animal models involving non-invasively induced, localized neural damage are limited. We investigated an in vivo murine hematoma model in which cerebral hemorrhage was induced noninvasively by high-intensity focused ultrasound (HIFU) with calibrated positioning and parameters. To characterize the morphology of induced hematomas, we used skull-intact histological evaluation. A multi-wavelength fiber-optic NIRS system with three source-detector separation distances was used to detect hematoma A 1.1 MHz transducer produced consistent small-to-medium hematoma localized to a single hemisphere, along with bruising of the scalp, with a low mortality rate. A 220 kHz transducer produced larger, more diffuse hematomas, with higher variability in size and a correspondingly higher mortality rate. No skin bruising or blood accumulation between the skin and skull was observed following injury application with the 220 kHz transducer. Histological analysis showed higher sensitivity for larger hematomas (>4x4 mm2). NIRS optical density change after HIFU was able to detect all hematomas, with sensitivity dependent on wavelength and separation distance. While improvements in methods for validating cerebral blood distribution are needed, the HIFU hematoma model provided useful insights that will inform development of biologically relevant, performance test methods for cerebral NIRS systems.

Upregulated epidermal growth factor receptor expression following near-infrared irradiation simulating solar radiation in a three-dimensional reconstructed human corneal epithelial tissue culture model

PubMed Central

Tanaka, Yohei; Nakayama, Jun

2016-01-01

Background and objective Humans are increasingly exposed to near-infrared (NIR) radiation from both natural (eg, solar) and artificial (eg, electrical appliances) sources. Although the biological effects of sun and ultraviolet (UV) exposure have been extensively investigated, the biological effect of NIR radiation is still unclear. We previously reported that NIR as well as UV induces photoaging and standard UV-blocking materials, such as sunglasses, do not sufficiently block NIR. The objective of this study was to investigate changes in gene expression in three-dimensional reconstructed corneal epithelial tissue culture exposed to broad-spectrum NIR irradiation to simulate solar NIR radiation that reaches human tissues. Materials and methods DNA microarray and quantitative real-time polymerase chain reaction analysis were used to assess gene expression levels in a three-dimensional reconstructed corneal epithelial model composed of normal human corneal epithelial cells exposed to water-filtered broad-spectrum NIR irradiation with a contact cooling (20°C). The water-filter allowed 1,000–1,800 nm wavelengths and excluded 1,400–1,500 nm wavelengths. Results A DNA microarray with >62,000 different probes showed 25 and 150 genes that were up- or downregulated by at least fourfold and twofold, respectively, after NIR irradiation. In particular, epidermal growth factor receptor (EGFR) was upregulated by 19.4-fold relative to control cells. Quantitative real-time polymerase chain reaction analysis revealed that two variants of EGFR in human corneal epithelial tissue were also significantly upregulated after five rounds of 10 J/cm2 irradiation (P<0.05). Conclusion We found that NIR irradiation induced the upregulated expression of EGFR in human corneal cells. Since over half of the solar energy reaching the Earth is in the NIR region, which cannot be adequately blocked by eyewear and thus can induce eye damage with intensive or long-term exposure, protection from both UV and NIR radiation may prevent changes in gene expression and in turn eye damage. PMID:27536083

Design of a miniaturized integrated spectrometer for spectral tissue sensing

NASA Astrophysics Data System (ADS)

Belay, Gebirie Yizengaw; Hoving, Willem; Ottevaere, Heidi; van der Put, Arthur; Weltjens, Wim; Thienpont, Hugo

2016-04-01

Minimally-invasive image-guided procedures become increasingly used by physicians to obtain real-time characterization feedback from the tissue at the tip of their interventional device (needle, catheter, endoscopic or laparoscopic probes, etc…) which can significantly improve the outcome of diagnosis and treatment, and ultimately reduce cost of the medical treatment. Spectral tissue sensing using compact photonic probes has the potential to be a valuable tool for screening and diagnostic purposes, e.g. for discriminating between healthy and tumorous tissue. However, this technique requires a low-cost broadband miniature spectrometer so that it is commercially viable for screening at point-of-care locations such as physicians' offices and outpatient centers. Our goal is therefore to develop a miniaturized spectrometer based on diffractive optics that combines the functionalities of a visible/near-infrared (VIS/NIR) and shortwave-infrared (SWIR) spectrometer in one very compact housing. A second goal is that the hardware can be produced in high volume at low cost without expensive time consuming alignment and calibration steps. We have designed a miniaturized spectrometer which operates both in the visible/near-infrared and shortwave-infrared wavelength regions ranging from 400 nm to 1700 nm. The visible/near-infrared part of the spectrometer is designed for wavelengths from 400 nm to 800 nm whereas the shortwave-infrared segment ranges from 850 nm to 1700 nm. The spectrometer has a resolution of 6 nm in the visible/near-infrared wavelength region and 10 nm in the shortwave-infrared. The minimum SNR of the spectrometer for the intended application is about 151 in the VIS/NIR range and 6000 for SWIR. In this paper, the modelling and design, and power budget analysis of the miniaturized spectrometer are presented. Our work opens a door for future affordable micro- spectrometers which can be integrated with smartphones and tablets, and used for point-of-care applications. As next steps in the development, we will manufacture the different optical components and experimentally characterize the spectrometer device in more detail.

Prediction of olive quality using FT-NIR spectroscopy in reflectance and transmittance modes

USDA-ARS?s Scientific Manuscript database

The objective of this research was to use FT-NIR spectroscopy to predict the firmness, oil content and color of two olive (Olea europaea L) varieties (‘Ayvalik’ and ‘Gemlik’). Spectral measurements were performed on the intact olives for the wavelengths of 780-2500 nm in reflectance and for 800-1725...

Characterization of a reflective objective with multiphoton microscopy

NASA Astrophysics Data System (ADS)

Kabir, Mohammad M.; Choubal, Aakash M.; Sivaguru, Mayandi; Toussaint, Kimani C.

2018-02-01

Reflective objectives (ROs) can reduce chromatic aberration across a wide wavelength range in multiphoton microscopy (MPM). However, a systematic characterization of the performance of ROs has not been carried out. In this paper, we analyze the performance of a 0.5 numerical-aperture (NA) RO and compare it with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments extending 1 octave in visible and NIR wavelengths, the SO introduces defocusing errors of 25% for TPF images of sub-diffraction fluorescent beads and 10% for SHG images of collagen fibers. For both imaging systems, the RO provides a corresponding error of 4%. This work highlights the potential usefulness of ROs for multimodal MPM applications.

Mutations in GPR143/OA1 and ABCA4 Inform Interpretations of Short-Wavelength and Near-Infrared Fundus Autofluorescence

PubMed Central

Paavo, Maarjaliis; Zhao, Jin; Kim, Hye Jin; Lee, Winston; Zernant, Jana; Cai, Carolyn; Allikmets, Rando; Tsang, Stephen H.; Sparrow, Janet R.

2018-01-01

Purpose We sought to advance interpretations and quantification of short-wavelength fundus autofluorescence (SW-AF) emitted from bisretinoid lipofuscin and near-infrared autofluoresence (NIR-AF) originating from melanin. Methods Carriers of mutations in X-linked GPR143/OA1, a common form of ocular albinism; patients with confirmed mutations in ABCA4 conferring increased SW-AF; and subjects with healthy eyes were studied. SW-AF (488 nm excitation, 500–680 nm emission) and NIR-AF (excitation 787 nm, emission >830 nm) images were acquired with a confocal scanning laser ophthalmoscope. SW-AF images were analyzed for quantitative autofluoresence (qAF). Analogous methods of image acquisition and analysis were performed in albino and pigmented Abca4−/− mice and wild-type mice. Results Quantitation of SW-AF (qAF), construction of qAF color-coded maps, and examination of NIR-AF images from GPR143/OA1 carriers revealed mosaics in which patches of fundus exhibiting NIR-AF signal had qAF levels within normal limits whereas the hypopigmented areas in the NIR-AF image corresponded to foci of elevated qAF. qAF also was increased in albino versus pigmented mice. Although melanin contributes to fundus infrared reflectance, the latter appeared to be uniform in en face reflectance images of GPR143/OA1-carriers. In patients diagnosed with ABCA4-associated disease, NIR-AF increased in tandem with increased qAF originating in bisretinoid lipofuscin. Similarly in Abca4−/− mice having increased SW-AF, NIR-AF was more pronounced than in wild-type mice. Conclusions These studies corroborate RPE melanin as the major source of NIR-AF but also indicate that bisretinoid lipofuscin, when present at sufficient concentrations, contributes to the NIR-AF signal. Ocular melanin attenuates the SW-AF signal.

SHARK-NIR: from K-band to a key instrument, a status update

NASA Astrophysics Data System (ADS)

Farinato, Jacopo; Bacciotti, Francesca; Baffa, Carlo; Baruffolo, Andrea; Bergomi, Maria; Bongiorno, Angela; Carbonaro, Luca; Carolo, Elena; Carlotti, Alexis; Centrone, Mauro; Close, Laird; De Pascale, Marco; Dima, Marco; D'Orazi, Valentina; Esposito, Simone; Fantinel, Daniela; Farisato, Giancarlo; Gaessler, Wolfgang; Giallongo, Emanuele; Greggio, Davide; Guyon, Olivier; Hinz, Philip; Lisi, Franco; Magrin, Demetrio; Marafatto, Luca; Mohr, Lars; Montoya, Manny; Pedichini, Fernando; Pinna, Enrico; Puglisi, Alfio; Ragazzoni, Roberto; Salasnich, Bernardo; Stangalini, Marco; Vassallo, Daniele; Verinaud, Christophe; Viotto, Valentina

2016-07-01

SHARK-NIR channel is one of the two coronagraphic instruments proposed for the Large Binocular Telescope, in the framework of the call for second generation instruments, issued in 2014. Together with the SHARK-VIS channel, it will offer a few observing modes (direct imaging, coronagraphic imaging and coronagraphic low resolution spectroscopy) covering a wide wavelength domain, going from 0.5μm to 1.7μm. Initially proposed as an instrument covering also the K-band, the current design foresees a camera working from Y to H bands, exploiting in this way the synergy with other LBT instruments such as LBTI, which is actually covering wavelengths greater than L' band, and it will be soon upgraded to work also in K band. SHARK-NIR has been undergoing the conceptual design review at the end of 2015 and it has been approved to proceed to the final design phase, receiving the green light for successive construction and installation at LBT. The current design is significantly more flexible than the previous one, having an additional intermediate pupil plane that will allow the usage of coronagraphic techniques very efficient in term of contrast and vicinity to the star, increasing the instrument coronagraphic performance. The latter is necessary to properly exploit the search of giant exo-planets, which is the main science case and the driver for the technical choices of SHARK-NIR. We also emphasize that the LBT AO SOUL upgrade will further improve the AO performance, making possible to extend the exo-planet search to target fainter than normally achieved by other 8-m class telescopes, and opening in this way to other very interesting scientific scenarios, such as the characterization of AGN and Quasars (normally too faint to be observed) and increasing considerably the sample of disks and jets to be studied. Finally, we emphasize that SHARK-NIR will offer XAO direct imaging capability on a FoV of about 15"x15", and a simple coronagraphic spectroscopic mode offering spectral resolution ranging from few hundreds to few thousands. This article presents the current instrument design, together with the milestones for its installation at LBT.

Phase-dependent Photometric and Spectroscopic Characterization of the MASTER-Net Optical Transient J212444.87+321738.3: An Oxygen-rich Mira

NASA Astrophysics Data System (ADS)

Ghosh, Supriyo; Mondal, Soumen; Das, Ramkrishna; Banerjee, D. P. K.; Ashok, N. M.; Hambsch, Franz-Josef; Dutta, Somnath

2018-05-01

We describe the time-dependent properties of a new spectroscopically confirmed Mira variable, which was discovered in 2013 as MASTER-Net Optical Transient J212444.87+321738.3 toward the Cygnus constellation. We have performed long-term optical/near-infrared (NIR) photometric and spectroscopic observations to characterize the object. From the optical/NIR light curves, we estimate a variability period of 465 ± 30 days. The wavelength-dependent amplitudes of the observed light curves range from ΔI ∼ 4 mag to ΔK ∼ 1.5 mag. The (J ‑ K) color index varies from 1.78 to 2.62 mag over phases. Interestingly, a phase lag of ∼60 days between optical and NIR light curves is also seen, as in other Miras. Our optical/NIR spectra show molecular features of TiO, VO, CO, and strong water bands that are a typical signature of oxygen-rich Mira. We rule out S- or C-type as ZrO bands at 1.03 and 1.06 μm and C2 band at 1.77 μm are absent. We estimate the effective temperature of the object from the Spectral Energy Distribution, and distance and luminosity from standard Period–Luminosity relations. The optical/NIR spectra display time-dependent atomic and molecular features (e.g., TiO, Na I, Ca I, H2O, CO), as commonly observed in Miras. Such spectroscopic observations are useful for studying pulsation variability in Miras.

Transdermal gelation of methacrylated macromers with near-infrared light and gold nanorods

NASA Astrophysics Data System (ADS)

Gramlich, William M.; Holloway, Julianne L.; Rai, Reena; Burdick, Jason A.

2014-01-01

Injectable hydrogels provide locally controlled tissue bulking and a means to deliver drugs and cells to the body. The formation of hydrogels in vivo may involve the delivery of two solutions that spontaneously crosslink when mixed, with pH or temperature changes, or with light (e.g., visible or ultraviolet). With these approaches, control over the kinetics of gelation, introduction of the initiation trigger (e.g., limited penetration of ultraviolet light through tissues), or alteration of the material physical properties (e.g., mechanics) may be difficult to achieve. To overcome these limitations, we used the interaction of near-infrared (NIR) light with gold nanorods (AuNRs) to generate heat through the photothermal effect. NIR light penetrates tissues to a greater extent than other wavelengths and provides a means to indirectly initiate radical polymerization. Specifically, this heating coupled with a thermal initiator (VA-044) produced radicals that polymerized methacrylated hyaluronic acid (MeHA) and generated hydrogels. A range of VA-044 concentrations changed the gelation time, yielding a system stable at 37 ° C for 22 min that gels quickly (˜3 min) when heated to 55 ° C. With a constant irradiation time (10 min) and laser power (0.3 W), different VA-044 and AuNR concentrations tuned the compressive modulus of the hydrogel. By changing the NIR irradiation time we attained a wide range of moduli at a set solution composition. In vivo mouse studies confirmed that NIR laser irradiation through tissue could gel an injected precursor solution transdermally.

Systematization method for distinguishing plastic groups by using NIR spectroscopy.

PubMed

Kaihara, Mikio; Satoh, Minami; Satoh, Minoru

2007-07-01

A systematic classification method for polymers is not yet available in case of using near infrared spectra (NIR). That is why we have been searching for a systematic method. Because raw NIR spectra usually have few obvious peaks, NIR spectra have been pretreated by 2nd derivation for taking well modulated spectra. After the pretreatment, we applied classification and regression trees (CART) to the discrimination between the spectra and the species of polymers. As a result, we obtained a relatively simple classification tree. Judging from the obtained splitting conditions and the classified polymers, we concluded that obtained knowledge on the chemical function groups estimated by the important wavelength regions is not always applicable to this classification tree. However, we clarified the splitting rules for polymer species from the NIR spectral point of view.

Optical sideband generation up to room temperature with mid-infrared quantum cascade lasers.

PubMed

Houver, S; Cavalié, P; St-Jean, M Renaudat; Amanti, M I; Sirtori, C; Li, L H; Davies, A G; Linfield, E H; Pereira, T A S; Lebreton, A; Tignon, J; Dhillon, S S

2015-02-23

Mid-infrared (MIR) sideband generation on a near infrared (NIR) optical carrier is demonstrated within a quantum cascade laser (QCL). By employing an externally injected NIR beam, E(NIR), that is resonant with the interband transitions of the quantum wells in the QCL, the nonlinear susceptibility is enhanced, leading to both frequency mixing and sideband generation. A GaAs-based MIR QCL (E(QCL) = 135 meV) with an aluminum-reinforced waveguide was utilized to overlap the NIR and MIR modes with the optical nonlinearity of the active region. The resulting difference sideband (E(NIR) - E(QCL)) shows a resonant behavior as a function of NIR pump wavelength and a maximum second order nonlinear susceptibility, χ((2)), of ~1 nm/V was obtained. Further, the sideband intensity showed little dependence with the operating temperature of the QCL, allowing sideband generation to be realized at room temperature.

Indium phosphide all air-gap Fabry-Pérot filters for near-infrared spectroscopic applications

NASA Astrophysics Data System (ADS)

Ullah, A.; Butt, M. A.; Fomchenkov, S. A.; Khonina, S. N.

2016-08-01

Food quality can be characterized by noninvasive techniques such as spectroscopy in the Near Infrared wavelength range. For example, 930 -1450 nm wavelength range can be used to detect diseases and differentiate between meat samples. Miniaturization of such NIR spectrometers is useful for quick and mobile characterization of food samples. Spectrometers can be miniaturized, without compromising the spectral resolution, using Fabry-Pérot (FP) filters consisting of two highly reflecting mirrors with a central cavity in between. The most commonly used mirrors in the design of FP filters are Distributed Bragg Reflections (DBRs) consisting of alternating high and low refractive index material pairs, due to their high reflectivity compared to metal mirrors. However, DBRs have high reflectivity for a selected range of wavelengths known as the stopband of the DBR. This range is usually much smaller than the sensitivity range of the spectrometer detector. Therefore, a bandpass filter is usually required to restrict wavelengths outside the stopband of the FP DBRs. Such bandpass filters are difficult to design and implement. Alternatively, high index contrast materials must be can be used to broaden the stopband width of the FP DBRs. In this work, Indium phosphide all air-gap filters are proposed in conjunction with InGaAs based detectors. The designed filter has a wide stopband covering the entire InGaAs detector sensitivity range. The filter can be tuned in the 950-1450 nm with single mode operation. The designed filter can hence be used for noninvasive meat quality control.

A study of aerosol absorption and height retrievals with a hyperspectral (UV to NIR) passive sensor

NASA Astrophysics Data System (ADS)

Gasso, S.

2017-12-01

With the deployment of the first sensor (TOMS, in 1978) with capabilities to detect aerosol absorption (AA) from space, there has been a continuous evolution in hardware and algorithms used to measured this property. Although with TOMS and its more advanced successors (such as OMI) made significant progress in globally characterizing AA , there is room for improvement especially by taking advantage of sensors with extended spectral coverage (UV to NIR) and high spatial resolution (<1 km). While such unique sensor does not exist yet, the collocation of observations from different platforms that jointly fulfill those characteristics (e.g. A-Train, S-NPP) confirm that it is possible to fully retrieve all AA parameters that modulate absorption in the upwelling radiance (AOD, SSA and aerosol layer height). However, such combined approaches still have some drawbacks such as the difficulty to account for cloud contamination. The upcoming deployment of satellite detectors with the desired features all in one sensor (PACE, TropOMI, GEMS) prompt a revision of the AA retrieval technique used in past approaches. In particular,the TropOMI mission, a hyperspectral UV-to-NIR sensor with moderate ( 5km nadir pixel) spatial resolution to be launched in Fall 2017. In addition , the sensor will include sensing capabilities for the wavelength range of the Oxygen bands A and B at very high wavelength resolution. This study will be centered on the aerosol detection capabilities of TropOMI. Because the spectral range covered, it is theoretically possible to simultaneously retrieve the aerosol optical depth, the single scattering albedo and aerosol mean height without assuming any of them as it was the case with previous retrieval approaches. Specifically, we intend to present a theoretical study based on simulated radiances at selected UV, VIS and near-IR bands (including the Oxygen bands) and evaluate the sensitivity of this sensor to different levels of aerosol concentration, height and absorption properties (imaginary index) along with particle size distribution.

Differential Effects of 670 and 830 nm Red near Infrared Irradiation Therapy: A Comparative Study of Optic Nerve Injury, Retinal Degeneration, Traumatic Brain and Spinal Cord Injury

PubMed Central

Giacci, Marcus K.; Wheeler, Lachlan; Lovett, Sarah; Dishington, Emma; Majda, Bernadette; Bartlett, Carole A.; Thornton, Emma; Harford-Wright, Elizabeth; Leonard, Anna; Vink, Robert; Harvey, Alan R.; Provis, Jan; Dunlop, Sarah A.; Fitzgerald, Melinda

2014-01-01

Red/near-infrared irradiation therapy (R/NIR-IT) delivered by laser or light-emitting diode (LED) has improved functional outcomes in a range of CNS injuries. However, translation of R/NIR-IT to the clinic for treatment of neurotrauma has been hampered by lack of comparative information regarding the degree of penetration of the delivered irradiation to the injury site and the optimal treatment parameters for different CNS injuries. We compared the treatment efficacy of R/NIR-IT at 670 nm and 830 nm, provided by narrow-band LED arrays adjusted to produce equal irradiance, in four in vivo rat models of CNS injury: partial optic nerve transection, light-induced retinal degeneration, traumatic brain injury (TBI) and spinal cord injury (SCI). The number of photons of 670 nm or 830 nm light reaching the SCI injury site was 6.6% and 11.3% of emitted light respectively. Treatment of rats with 670 nm R/NIR-IT following partial optic nerve transection significantly increased the number of visual responses at 7 days after injury (P≤0.05); 830 nm R/NIR-IT was partially effective. 670 nm R/NIR-IT also significantly reduced reactive species and both 670 nm and 830 nm R/NIR-IT reduced hydroxynonenal immunoreactivity (P≤0.05) in this model. Pre-treatment of light-induced retinal degeneration with 670 nm R/NIR-IT significantly reduced the number of Tunel+ cells and 8-hydroxyguanosine immunoreactivity (P≤0.05); outcomes in 830 nm R/NIR-IT treated animals were not significantly different to controls. Treatment of fluid-percussion TBI with 670 nm or 830 nm R/NIR-IT did not result in improvements in motor or sensory function or lesion size at 7 days (P>0.05). Similarly, treatment of contusive SCI with 670 nm or 830 nm R/NIR-IT did not result in significant improvements in functional recovery or reduced cyst size at 28 days (P>0.05). Outcomes from this comparative study indicate that it will be necessary to optimise delivery devices, wavelength, intensity and duration of R/NIR-IT individually for different CNS injury types. PMID:25105800

Ability of near infrared spectroscopy to monitor air-dry density distribution and variation of wood

Treesearch

Brian K. Via; Chi-Leung So; Todd F. Shupe; Michael Stine; Leslie H. Groom

2005-01-01

Process control of wood density with near infrared spectroscopy (NIR) would be useful for pulp mills that need to maximize pulp yield without compromising paper strength properties. If models developed from the absorbance at wavelengths in the NIR region could provide density histograms, fiber supply personnel could monitor chip density variation as the chips enter the...

A DAQ-Device-Based Continuous Wave Near-Infrared Spectroscopy System for Measuring Human Functional Brain Activity

PubMed Central

Li, Xiaoli; Liu, Xiaomin

2014-01-01

In the last two decades, functional near-infrared spectroscopy (fNIRS) is getting more and more popular as a neuroimaging technique. The fNIRS instrument can be used to measure local hemodynamic response, which indirectly reflects the functional neural activities in human brain. In this study, an easily implemented way to establish DAQ-device-based fNIRS system was proposed. Basic instrumentation components (light sources driving, signal conditioning, sensors, and optical fiber) of the fNIRS system were described. The digital in-phase and quadrature demodulation method was applied in LabVIEW software to distinguish light sources from different emitters. The effectiveness of the custom-made system was verified by simultaneous measurement with a commercial instrument ETG-4000 during Valsalva maneuver experiment. The light intensity data acquired from two systems were highly correlated for lower wavelength (Pearson's correlation coefficient r = 0.92, P < 0.01) and higher wavelength (r = 0.84, P < 0.01). Further, another mental arithmetic experiment was implemented to detect neural activation in the prefrontal cortex. For 9 participants, significant cerebral activation was detected in 6 subjects (P < 0.05) for oxyhemoglobin and in 8 subjects (P < 0.01) for deoxyhemoglobin. PMID:25180044

Continuous wave optical spectroscopic system for use in magnetic resonance imaging scanners for the measurement of changes in hemoglobin oxygenation states in humans

NASA Astrophysics Data System (ADS)

Hulvershorn, Justin; Bloy, Luke; Leigh, John S.; Elliott, Mark A.

2003-09-01

A continuous wave near infrared three-wavelength laser diode spectroscopic (NIRS) system designed for use in magnetic resonance imaging (MRI) scanners is described. This system measures in vivo changes in the concentrations of oxyhemoglobin (HbO) and deoxyhemoglobin (Hb) in humans. An algorithm is implemented to map changes in light intensity to changes in the concentrations of Hb and HbO. The system's signal to noise ratio is 3.4×103 per wavelength on an intralipid phantom with 10 Hz resolution. To demonstrate the system's performance in vivo, data taken on the human forearm during arterial occlusion, as well as data taken on the forehead during extended breath holds, are presented. The results show that the instrument is an extremely sensitive detector of hemodynamic changes in human tissue at high temporal resolution. NIRS directly measures changes in the concentrations of hemoglobin species. For this reason, NIRS will be useful in determining the sources of MRI signal changes in the body due to hemodynamic causes, while the precise anatomic information provided by MRI will aid in localizing NIRS contrast and improving the accuracy of models of light transport through tissue.

Design of a simple non-destructive detection system using P-wave lasers for determining the soluble solids content of apples.

PubMed

Hua, Shih-Hao; Chen, Chao-Pin; Han, Pin

2017-08-01

The simple and nondestructive detection system studied in this work uses a near-infrared (NIR) detector and parallel-polarized (P-wave) NIR lasers to determine the soluble solids content (SSC) of apples. The P-wave NIR laser in this system is incident into the apple's pulp at the Brewster angle to minimize the interference caused by interfacial reflections. After the apple has been illuminated by four P-wave NIR lasers that correspond to the specified wavelengths of the SSC chemical bonds (880, 940, 980, and 1064 nm), the prediction of correlation (rp2) and the root-mean-square error for prediction (RMSEP) of the SSC are determined via partial least square regression analysis of the reflectance. Our results indicate that the use of P-wave lasers at the Brewster angle (as the angle of incidence) and the above specified wavelengths for the prediction set measurement of the SSC of apples obtained an rp2 of 0.88 and an RMSEP of 0.47°Brix. These rp2 are 6% higher, and the RMSEPs are 9% lower, than those obtained using non-polarized lasers.

Explaining Space-Weathering Effects on UV-Vis-NIR Spectra with Light-Scattering Methods

NASA Astrophysics Data System (ADS)

Penttilä, Antti; Väisänen, Timo; Martikainen, Julia; Kohout, Tomas; Muinonen, Karri

2015-11-01

Space-weathering (SW) introduces changes to the asteroid reflectance spectra. In silicate minerals, SW is known to darken the spectra and reduce the silicate absorption band depths. In olivine, the neutral slope in Vis and NIR wavelengths is becoming positive [1]. In pyroxene, the positive slope over the 1 µm absorption band is decreasing, and the negative slope over the 2 µm band is increasing towards positive values with increasing SW [2].The SW process generates small nanophase iron (npFe0) inclusions in the surface layers of mineral grains. The inclusions are some tens of nm in size. This mechanism has been linked to the Moon and to a certain extent also to the silicate-rich S-complex asteroids.We offer two simple explanations from light-scattering theory to explain the SW effects on the spectral slope. First, the npFe0 will introduce a posititive general slope (reddening) to the spectra. The npFe0 inclusions (~10 nm) are in the Rayleigh domain with the wavelength λ in the UV-Vis-NIR range. Their absorption cross-section follows approximately the 1/λ-relation from the Rayleigh theory. Absorption is more efficient in the UV than in the NIR wavelengths, therefore the spectra are reddening.Second, the effect of npFe0 absorption is more efficient for originally brighter reflectance values. Explanation combines the effective medium theory and the exponential attenuation in the medium. When adding a small amount of highly absorbing npFe0, the effective absorption coefficient k will increase approximately the same Δk for the typical values of silicates. This change will increase more effectively the exponential attenuation if the original k was very small, and thus the reflectance high. Therefore, both positive and negative spectral slopes will approach zero with SW.We conclude that the SW will introduce a general reddening, and neutralize local slopes. This is verified using the SIRIS code [3], which combines geometric optics with small internal diffuse scatterers in the radiative transfer domain.[1] Kohout T. et al. (2014), Icarus 237(15), 75-83.[2] Kohout T. et al. (2015), Workshop on Space Weathering of Airless Bodies, Abstract.[3] Muinonen K. et al. (2009), JQSRT 110, 1628-1639.

Enhancement of near-infrared detectability from InGaZnO thin film transistor with MoS2 light absorbing layer.

PubMed

Pak, Sang Woo; Chu, Dongil; Song, Da Ye; Lee, Seung Kyo; Kim, Eun Kyu

2017-11-24

We report an enhancement of near-infrared (NIR) detectability from amorphous InGaZnO (α-IGZO) thin film transistor in conjunction with randomly distributed molybdenum disulfide (MoS 2 ) flakes. The electrical characteristics of the α-IGZO grown by radio-frequency magnetron sputtering exhibit high effective mobility exceeding 15 cm 2 V -1 s -1 and current on/off ratio up to 10 7 . By taking advantages of the high quality α-IGZO and MoS 2 light absorbing layer, photodetection spectra are able to extend from ultra-violet to NIR range. The α-IGZO channel detector capped by MoS 2 show a photo-responsivity of approximately 14.9 mA W -1 at 1100 nm wavelength, which is five times higher than of the α-IGZO device without MoS 2 layer.

Enhancement of near-infrared detectability from InGaZnO thin film transistor with MoS2 light absorbing layer

NASA Astrophysics Data System (ADS)

Pak, Sang Woo; Chu, Dongil; Song, Da Ye; Kyo Lee, Seung; Kim, Eun Kyu

2017-11-01

We report an enhancement of near-infrared (NIR) detectability from amorphous InGaZnO (α-IGZO) thin film transistor in conjunction with randomly distributed molybdenum disulfide (MoS2) flakes. The electrical characteristics of the α-IGZO grown by radio-frequency magnetron sputtering exhibit high effective mobility exceeding 15 cm2 V-1 s-1 and current on/off ratio up to 107. By taking advantages of the high quality α-IGZO and MoS2 light absorbing layer, photodetection spectra are able to extend from ultra-violet to NIR range. The α-IGZO channel detector capped by MoS2 show a photo-responsivity of approximately 14.9 mA W-1 at 1100 nm wavelength, which is five times higher than of the α-IGZO device without MoS2 layer.

Dynamically Reconfigurable Metadevice Employing Nanostructured Phase-Change Materials

DOE PAGES

Zhu, Zhihua; Evans, Philip G.; Haglund, Richard F.; ...

2017-07-21

Mastering dynamic free-space spectral control and modulation in the near-infrared (NIR) and optical regimes remains a challenging task that is hindered by the available functional materials at high frequencies. In this work, we have realized an efficient metadevice capable of spectral control by minimizing the thermal mass of a vanadium dioxide phase-change material (PCM) and placing the PCM at the feed gap of a bow-tie field antenna. The device has an experimentally measured tuning range of up to 360 nm in the NIR and a modulation depth of 33% at the resonant wavelength. The metadevice is configured for integrated andmore » local heating, leading to faster switching and more precise spatial control compared with devices based on phase-change thin films. We envisage that the combined advantages of this device will open new opportunities for signal processing, memory, security, and holography at optical frequencies.« less

Dynamically Reconfigurable Metadevice Employing Nanostructured Phase-Change Materials.

PubMed

Zhu, Zhihua; Evans, Philip G; Haglund, Richard F; Valentine, Jason G

2017-08-09

Mastering dynamic free-space spectral control and modulation in the near-infrared (NIR) and optical regimes remains a challenging task that is hindered by the available functional materials at high frequencies. In this work, we have realized an efficient metadevice capable of spectral control by minimizing the thermal mass of a vanadium dioxide phase-change material (PCM) and placing the PCM at the feed gap of a bow-tie field antenna. The device has an experimentally measured tuning range of up to 360 nm in the NIR and a modulation depth of 33% at the resonant wavelength. The metadevice is configured for integrated and local heating, leading to faster switching and more precise spatial control compared with devices based on phase-change thin films. We envisage that the combined advantages of this device will open new opportunities for signal processing, memory, security, and holography at optical frequencies.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu, Zhihua; Evans, Philip G.; Haglund, Richard F.

Mastering dynamic free-space spectral control and modulation in the near-infrared (NIR) and optical regimes remains a challenging task that is hindered by the available functional materials at high frequencies. In this work, we have realized an efficient metadevice capable of spectral control by minimizing the thermal mass of a vanadium dioxide phase-change material (PCM) and placing the PCM at the feed gap of a bow-tie field antenna. The device has an experimentally measured tuning range of up to 360 nm in the NIR and a modulation depth of 33% at the resonant wavelength. The metadevice is configured for integrated andmore » local heating, leading to faster switching and more precise spatial control compared with devices based on phase-change thin films. We envisage that the combined advantages of this device will open new opportunities for signal processing, memory, security, and holography at optical frequencies.« less

Visible and near-infrared spectroscopic analysis of raw milk for cow health monitoring: reflectance or transmittance?

PubMed

Aernouts, B; Polshin, E; Lammertyn, J; Saeys, W

2011-11-01

The composition of produced milk has great value for the dairy farmer. It determines the economic value of the milk and provides valuable information about the metabolism of the corresponding cow. Therefore, online measurement of milk components during milking 2 or more times per day would provide knowledge about the current health and nutritional status of each cow individually. This information provides a solid basis for optimizing cow management. The potential of visible and near-infrared (Vis/NIR) spectroscopy for predicting the fat, crude protein, lactose, and urea content of raw milk online during milking was, therefore, investigated in this study. Two measurement modes (reflectance and transmittance) and different wavelength ranges for Vis/NIR spectroscopy were evaluated and their ability to measure the milk composition online was compared. The Vis/NIR reflectance measurements allowed for very accurate monitoring of the fat and crude protein content in raw milk (R(2)>0.95), but resulted in poor lactose predictions (R(2)<0.75). In contrast, Vis/NIR transmittance spectra of the milk samples gave accurate fat and crude protein predictions (R(2)>0.90) and useful lactose predictions (R(2)=0.88). Neither Vis/NIR reflectance nor transmittance spectroscopy lead to an acceptable prediction of the milk urea content. Transmittance spectroscopy can thus be used to predict the 3 major milk components, but with lower accuracy for fat and crude protein than the reflectance mode. Moreover, the small sample thickness (1mm) required for NIR transmittance measurement considerably complicates its online use. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Rapid discrimination and quantification of alkaloids in Corydalis Tuber by near-infrared spectroscopy.

PubMed

Lu, Hai-yan; Wang, Shi-sheng; Cai, Rui; Meng, Yu; Xie, Xin; Zhao, Wei-jie

2012-02-05

With the application of near-infrared spectroscopy (NIRS), a convenient and rapid method for determination of alkaloids in Corydalis Tuber extract and classification for samples from different locations have been developed. Five different samples were collected according to their geographical origin, 2-Der with smoothing point of 17 was applied as the spectral pre-treatment, and the 1st to scaling range algorithm was adjusted to be optimal approach, classification model was constructed over the wavelength range of 4582-4270 cm⁻¹, 5562-4976 cm⁻¹ and 7000-7467 cm⁻¹ with a great recognition rate. For prediction model, partial least squares (PLS) algorithm was utilized referring to HPLC-UV reference method, the optimum models were obtained after adjustment. Pre-processing methods of calibration models were COE for protopine and min-max normalization for palmatine and MSC for tetrahydropalmatine, respectively. The root mean square errors of cross-validation (RMSECV) for protopine, palmatine, tetrahydropalmatine were 0.884, 1.83, 3.23 mg/g. The correlation coefficients (R²) were 99.75, 98.41 and 97.34%. T test was applied, in the model of tetrahydropalmatine; there is no significant difference between NIR prediction and HPLC reference method at 95% confidence interval with t=0.746

Frequency domain near-infrared multiwavelength imager design using high-speed, direct analog-to-digital conversion

NASA Astrophysics Data System (ADS)

Zimmermann, Bernhard B.; Fang, Qianqian; Boas, David A.; Carp, Stefan A.

2016-01-01

Frequency domain near-infrared spectroscopy (FD-NIRS) has proven to be a reliable method for quantification of tissue absolute optical properties. We present a full-sampling direct analog-to-digital conversion FD-NIR imager. While we developed this instrument with a focus on high-speed optical breast tomographic imaging, the proposed design is suitable for a wide-range of biophotonic applications where fast, accurate quantification of absolute optical properties is needed. Simultaneous dual wavelength operation at 685 and 830 nm is achieved by concurrent 67.5 and 75 MHz frequency modulation of each laser source, respectively, followed by digitization using a high-speed (180 MS/s) 16-bit A/D converter and hybrid FPGA-assisted demodulation. The instrument supports 25 source locations and features 20 concurrently operating detectors. The noise floor of the instrument was measured at <1.4 pW/√Hz, and a dynamic range of 115+ dB, corresponding to nearly six orders of magnitude, has been demonstrated. Titration experiments consisting of 200 different absorption and scattering values were conducted to demonstrate accurate optical property quantification over the entire range of physiologically expected values.

Frequency domain near-infrared multiwavelength imager design using high-speed, direct analog-to-digital conversion

PubMed Central

Zimmermann, Bernhard B.; Fang, Qianqian; Boas, David A.; Carp, Stefan A.

2016-01-01

Abstract. Frequency domain near-infrared spectroscopy (FD-NIRS) has proven to be a reliable method for quantification of tissue absolute optical properties. We present a full-sampling direct analog-to-digital conversion FD-NIR imager. While we developed this instrument with a focus on high-speed optical breast tomographic imaging, the proposed design is suitable for a wide-range of biophotonic applications where fast, accurate quantification of absolute optical properties is needed. Simultaneous dual wavelength operation at 685 and 830 nm is achieved by concurrent 67.5 and 75 MHz frequency modulation of each laser source, respectively, followed by digitization using a high-speed (180  MS/s) 16-bit A/D converter and hybrid FPGA-assisted demodulation. The instrument supports 25 source locations and features 20 concurrently operating detectors. The noise floor of the instrument was measured at <1.4  pW/√Hz, and a dynamic range of 115+ dB, corresponding to nearly six orders of magnitude, has been demonstrated. Titration experiments consisting of 200 different absorption and scattering values were conducted to demonstrate accurate optical property quantification over the entire range of physiologically expected values. PMID:26813081

Hot electron induced NIR detection in CdS films.

PubMed

Sharma, Alka; Kumar, Rahul; Bhattacharyya, Biplab; Husale, Sudhir

2016-03-11

We report the use of random Au nanoislands to enhance the absorption of CdS photodetectors at wavelengths beyond its intrinsic absorption properties from visible to NIR spectrum enabling a high performance visible-NIR photodetector. The temperature dependent annealing method was employed to form random sized Au nanoparticles on CdS films. The hot electron induced NIR photo-detection shows high responsivity of ~780 mA/W for an area of ~57 μm(2). The simulated optical response (absorption and responsivity) of Au nanoislands integrated in CdS films confirms the strong dependence of NIR sensitivity on the size and shape of Au nanoislands. The demonstration of plasmon enhanced IR sensitivity along with the cost-effective device fabrication method using CdS film enables the possibility of economical light harvesting applications which can be implemented in future technological applications.

Wavelength dependence of laser-induced retinal injury

NASA Astrophysics Data System (ADS)

Lund, David J.; Edsall, Peter; Stuck, Bruce E.

2005-04-01

The threshold for laser-induced retinal damage is dependent primarily upon the laser wavelength and the exposure duration. The study of the wavelength dependence of the retinal damage threshold has been greatly enhanced by the availability of tunable lasers. The Optical Parametric Oscillator (OPO), capable of providing useful pulse energy throughout a tuning range from 400 nm to 2200 nm, made it possible to determine the wavelength dependence of laser-induced retinal damage thresholds for q-switched pulses throughout the visible and NIR spectrum. Studies using the a tunable TI:Saph laser and several fixed-wavelength lasers yielded threshold values for 0.1 s exposures from 440 nm to 1060 nm. Laser-induced retinal damage for these exposure durations results from thermal conversion of the incident laser irradiation and an action spectrum for thermal retinal damage was developed based on the wavelength dependent transmission and absorption of ocular tissue and chromatic aberration of the eye optics. Long (1-1000s) duration exposures to visible laser demonstrated the existence of non-thermal laser-induced retinal damage mechanisms having a different action spectrum. This paper will present the available data for the wavelength dependence of laser-induced thermal retinal damage and compare this data to the maximum permissible exposure levels (MPEs) provided by the current guidelines for the safe use of lasers.

Robust Behavior Recognition in Intelligent Surveillance Environments.

PubMed

Batchuluun, Ganbayar; Kim, Yeong Gon; Kim, Jong Hyun; Hong, Hyung Gil; Park, Kang Ryoung

2016-06-30

Intelligent surveillance systems have been studied by many researchers. These systems should be operated in both daytime and nighttime, but objects are invisible in images captured by visible light camera during the night. Therefore, near infrared (NIR) cameras, thermal cameras (based on medium-wavelength infrared (MWIR), and long-wavelength infrared (LWIR) light) have been considered for usage during the nighttime as an alternative. Due to the usage during both daytime and nighttime, and the limitation of requiring an additional NIR illuminator (which should illuminate a wide area over a great distance) for NIR cameras during the nighttime, a dual system of visible light and thermal cameras is used in our research, and we propose a new behavior recognition in intelligent surveillance environments. Twelve datasets were compiled by collecting data in various environments, and they were used to obtain experimental results. The recognition accuracy of our method was found to be 97.6%, thereby confirming the ability of our method to outperform previous methods.

Evaluation of light detector surface area for functional Near Infrared Spectroscopy.

PubMed

Wang, Lei; Ayaz, Hasan; Izzetoglu, Meltem; Onaral, Banu

2017-10-01

Functional Near Infrared Spectroscopy (fNIRS) is an emerging neuroimaging technique that utilizes near infrared light to detect cortical concentration changes of oxy-hemoglobin and deoxy-hemoglobin non-invasively. Using light sources and detectors over the scalp, multi-wavelength light intensities are recorded as time series and converted to concentration changes of hemoglobin via modified Beer-Lambert law. Here, we describe a potential source for systematic error in the calculation of hemoglobin changes and light intensity measurements. Previous system characterization and analysis studies looked into various fNIRS parameters such as type of light source, number and selection of wavelengths, distance between light source and detector. In this study, we have analyzed the contribution of light detector surface area to the overall outcome. Results from Monte Carlo based digital phantoms indicated that selection of detector area is a critical system parameter in minimizing the error in concentration calculations. The findings here can guide the design of future fNIRS sensors. Copyright © 2017 Elsevier Ltd. All rights reserved.

Fluorescence lifetime FRET imaging of receptor-ligand complexes in tumor cells in vitro and in vivo

NASA Astrophysics Data System (ADS)

Rudkouskaya, Alena; Sinsuebphon, Nattawut; Intes, Xavier; Mazurkiewicz, Joseph E.; Barroso, Margarida

2017-02-01

To guide the development of targeted therapies with improved efficacy and accelerated clinical acceptance, novel imaging methodologies need to be established. Toward this goal, fluorescence lifetime Förster resonance energy transfer (FLIM-FRET) imaging assays capitalize on the ability of antibodies or protein ligands to bind dimerized membrane bound receptors to measure their target engagement levels in cancer cells. Conventional FLIM FRET microscopy has been widely applied at visible wavelengths to detect protein-protein interactions in vitro. However, operation at these wavelengths restricts imaging quality and ability to quantitate lifetime changes in in vivo small animal optical imaging due to high auto-fluorescence and light scattering. Here, we have analyzed the uptake of iron-bound transferrin (Tf) probes into human breast cancer cells using FLIM-FRET microscopy in the visible and near-infrared (NIR) range. The development of NIR FLIM FRET microscopy allows for the use of quantitative lifetime-based molecular assays to measure drug-target engagement levels at multiple scales: from in vitro microscopy to in vivo small animal optical imaging (macroscopy). This novel approach can be extended to other receptors, currently targeted in oncology. Hence, lifetime-based molecular imaging can find numerous applications in drug delivery and targeted therapy assessment and optimization.

Near-infrared Observations of SiO Maser-emitting Asymptotic Giant Branch (AGB) Stars

NASA Astrophysics Data System (ADS)

Chibueze, James O.; Miyahara, Takeshi; Omodaka, Toshihiro; Ohta, Takashi; Fujii, Takahiro; Tanaka, Masuo; Motohara, Kentaro; Makoto, Miyoshi

2016-02-01

Near-infrared (NIR) monitoring observations of asymptotic giant branch stars exciting bright SiO masers have been made with the 1 m telescope of Kagoshima University. In order to investigate the properties of these stars and their envelopes, we combined our NIR photometric data with mid- and far-infrared flux data obtained by the IRAS satellite, SiO maser flux data provided by the Nobeyama Radio Observatory, visual magnitude data provided by the AAVSO, and the reported data on the expansion velocities of the circumstellar envelopes. The absolute magnitudes at the K-band and the distances are estimated using the period-luminosity relation of Mira variables determined by Feast et al. Then, mass-loss rates and isotropic luminosities of an SiO maser are estimated. The mass-loss rates range from approximately 10-8 {M}⊙ \\{{yr}}-1 to over 10-5 {M}⊙ {{yr}}-1. We found that the NIR pulsation amplitudes are correlated with the pulsation periods and the observed wavelengths. We also found correlations of the isotropic luminosities of SiO masers with the mass-loss rates and absolute magnitudes at the K-band. These results will help us to understand the pumping mechanism of SiO masers. We measured, for the first time, the periods and/or NIR magnitudes of TX Cam, BW Cam, IRAS 06297+4045, IRAS 18387-0423, and RT Cep.

A Comparative Investigation of the Combined Effects of Pre-Processing, Wavelength Selection, and Regression Methods on Near-Infrared Calibration Model Performance.

PubMed

Wan, Jian; Chen, Yi-Chieh; Morris, A Julian; Thennadil, Suresh N

2017-07-01

Near-infrared (NIR) spectroscopy is being widely used in various fields ranging from pharmaceutics to the food industry for analyzing chemical and physical properties of the substances concerned. Its advantages over other analytical techniques include available physical interpretation of spectral data, nondestructive nature and high speed of measurements, and little or no need for sample preparation. The successful application of NIR spectroscopy relies on three main aspects: pre-processing of spectral data to eliminate nonlinear variations due to temperature, light scattering effects and many others, selection of those wavelengths that contribute useful information, and identification of suitable calibration models using linear/nonlinear regression . Several methods have been developed for each of these three aspects and many comparative studies of different methods exist for an individual aspect or some combinations. However, there is still a lack of comparative studies for the interactions among these three aspects, which can shed light on what role each aspect plays in the calibration and how to combine various methods of each aspect together to obtain the best calibration model. This paper aims to provide such a comparative study based on four benchmark data sets using three typical pre-processing methods, namely, orthogonal signal correction (OSC), extended multiplicative signal correction (EMSC) and optical path-length estimation and correction (OPLEC); two existing wavelength selection methods, namely, stepwise forward selection (SFS) and genetic algorithm optimization combined with partial least squares regression for spectral data (GAPLSSP); four popular regression methods, namely, partial least squares (PLS), least absolute shrinkage and selection operator (LASSO), least squares support vector machine (LS-SVM), and Gaussian process regression (GPR). The comparative study indicates that, in general, pre-processing of spectral data can play a significant role in the calibration while wavelength selection plays a marginal role and the combination of certain pre-processing, wavelength selection, and nonlinear regression methods can achieve superior performance over traditional linear regression-based calibration.

Application of a reflective microscope objective for multiphoton microscopy.

PubMed

Kabir, Mohammad M; Choubal, Aakash M; Toussaint, Kimani C

2018-04-20

Reflective objectives (ROs) mitigate chromatic aberration across a broad wavelength range. Yet, a systematic performance characterisation of ROs has not been done. In this paper, we compare the performance of a 0.5 numerical-aperture (NA) reflective objective (RO) with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments spanning ∼1 octave in the visible and NIR wavelengths, the SO leads to defocusing errors of 25-40% for TPF images of subdiffraction fluorescent beads and 10-12% for SHG images of collagen fibres. The corresponding error for the RO is ∼4% for both imaging modalities. This work emphasises the potential utility of ROs for multimodal multiphoton microscopy applications. © 2018 The Authors Journal of Microscopy © 2018 Royal Microscopical Society.

Near-infrared spectral image analysis of pork marbling based on Gabor filter and wide line detector techniques.

PubMed

Huang, Hui; Liu, Li; Ngadi, Michael O; Gariépy, Claude; Prasher, Shiv O

2014-01-01

Marbling is an important quality attribute of pork. Detection of pork marbling usually involves subjective scoring, which raises the efficiency costs to the processor. In this study, the ability to predict pork marbling using near-infrared (NIR) hyperspectral imaging (900-1700 nm) and the proper image processing techniques were studied. Near-infrared images were collected from pork after marbling evaluation according to current standard chart from the National Pork Producers Council. Image analysis techniques-Gabor filter, wide line detector, and spectral averaging-were applied to extract texture, line, and spectral features, respectively, from NIR images of pork. Samples were grouped into calibration and validation sets. Wavelength selection was performed on calibration set by stepwise regression procedure. Prediction models of pork marbling scores were built using multiple linear regressions based on derivatives of mean spectra and line features at key wavelengths. The results showed that the derivatives of both texture and spectral features produced good results, with correlation coefficients of validation of 0.90 and 0.86, respectively, using wavelengths of 961, 1186, and 1220 nm. The results revealed the great potential of the Gabor filter for analyzing NIR images of pork for the effective and efficient objective evaluation of pork marbling.

Red-edge position of habitable exoplanets around M-dwarfs.

PubMed

Takizawa, Kenji; Minagawa, Jun; Tamura, Motohide; Kusakabe, Nobuhiko; Narita, Norio

2017-08-08

One of the possible signs of life on distant habitable exoplanets is the red-edge, which is a rise in the reflectivity of planets between visible and near-infrared (NIR) wavelengths. Previous studies suggested the possibility that the red-edge position for habitable exoplanets around M-dwarfs may be shifted to a longer wavelength than that for Earth. We investigated plausible red-edge position in terms of the light environment during the course of the evolution of phototrophs. We show that phototrophs on M-dwarf habitable exoplanets may use visible light when they first evolve in the ocean and when they first colonize the land. The adaptive evolution of oxygenic photosynthesis may eventually also use NIR radiation, by one of two photochemical reaction centers, with the other center continuing to use visible light. These "two-color" reaction centers can absorb more photons, but they will encounter difficulty in adapting to drastically changing light conditions at the boundary between land and water. NIR photosynthesis can be more productive on land, though its evolution would be preceded by the Earth-type vegetation. Thus, the red-edge position caused by photosynthetic organisms on habitable M-dwarf exoplanets could initially be similar to that on Earth and later move to a longer wavelength.

NIST High Accuracy Reference Reflectometer-Spectrophotometer

PubMed Central

Proctor, James E.; Yvonne Barnes, P.

1996-01-01

A new reflectometer-spectrophotometer has been designed and constructed using state-of-the-art technology to enhance optical properties of materials measurements over the ultraviolet, visible, and near-infrared (UV-Vis-NIR) wavelength range (200 nm to 2500 nm). The instrument, Spectral Tri-function Automated Reference Reflectometer (STARR), is capable of measuring specular and diffuse reflectance, bidirectional reflectance distribution function (BRDF) of diffuse samples, and both diffuse and non-diffuse transmittance. Samples up to 30 cm by 30 cm can be measured. The instrument and its characterization are described. PMID:27805081

Near-infrared spectroscopy technique to evaluate the effects of drugs in treating traumatic brain edema

NASA Astrophysics Data System (ADS)

Xie, J.; Qian, Z.; Yang, T.; Li, W.; Hu, G.

2011-01-01

The aim of this study was to evaluate the effects of several drugs in treating traumatic brain edema (TBE) following traumatic brain injury (TBI) using near-infrared spectroscopy (NIRs) technology. Rats with TBE models were given hypertonic saline (HS), mannitol and mannitol+HS respectively for different groups. Light scattering properties of rat's local cortex was measured by NIRs within the wavelength range from 700 to 850 nm. TBE models were built in rats' left brains. The scattering properties of the right and left target corresponding to the position of normal and TBE tissue were measured and recorded in vivo and real-time by a bifurcated needle probe. The brain water contents (BWC) were measured by the wet and dry weight method after injury and treatment hours 1, 6, 24, 72 and 120. A marked linear relationship was observed between reduced scattering coefficient (μs') and BWC. By recording μs' of rats' brains, the entire progressions of effects of several drugs were observed. The result may suggest that the NIRs techniques have a potential for assessing effects in vivo and real-time on treatment of the brain injury.

Non-destructive geographical traceability of sea cucumber (Apostichopus japonicus) using near infrared spectroscopy combined with chemometric methods

PubMed Central

Cai, Rui; Wang, Shisheng; Tang, Bo; Li, Yueqing; Zhao, Weijie

2018-01-01

Sea cucumber is the major tonic seafood worldwide, and geographical origin traceability is an important part of its quality and safety control. In this work, a non-destructive method for origin traceability of sea cucumber (Apostichopus japonicus) from northern China Sea and East China Sea using near infrared spectroscopy (NIRS) and multivariate analysis methods was proposed. Total fat contents of 189 fresh sea cucumber samples were determined and partial least-squares (PLS) regression was used to establish the quantitative NIRS model. The ordered predictor selection algorithm was performed to select feasible wavelength regions for the construction of PLS and identification models. The identification model was developed by principal component analysis combined with Mahalanobis distance and scaling to the first range algorithms. In the test set of the optimum PLS models, the root mean square error of prediction was 0.45, and correlation coefficient was 0.90. The correct classification rates of 100% were obtained in both identification calibration model and test model. The overall results indicated that NIRS method combined with chemometric analysis was a suitable tool for origin traceability and identification of fresh sea cucumber samples from nine origins in China. PMID:29410795

Dark, Infrared Reflective, and Superhydrophobic Coatings by Waterborne Resins.

PubMed

Zhang, Jing; Lin, Weiqiang; Zhu, Chenxi; Lv, Jian; Zhang, Weicheng; Feng, Jie

2018-05-15

Recently, infrared reflective pigments possessing deep colors have attracted much attention. However, in polluted air, the coatings consisting of such pigments are easily contaminated which abates infrared reflectivity. In this work, black and infrared reflective pigments, fluorine silicon sol and a small number of SiO 2 nanoparticles were introduced into waterborne epoxy resin emulsion and then coated on an aluminum plate. After drying, black coatings with infrared reflective and superhydrophobic (SH) properties were obtained. The average near-infrared (NIR) reflectivity of the coating over wavelength range of 780-2600 nm can reach 68%, which is much larger than that of carbon black coatings and even approaches that of white nano SiO 2 coatings. Under the irradiation of a 275-W infrared lamp (with height 40 cm), the surface temperature of the coating is 63 °C, which is much lower than that of the carbon black coating (90 °C) and only 7 °C higher than that of the white nano SiO 2 coating. Furthermore, the NIR reflective coating exhibited a typical SH property due to its low surface energy and high surface roughness, which may allow for self-cleaning performance in a practical environment, maintaining the coating's NIR reflective property.

Non-destructive geographical traceability of sea cucumber (Apostichopus japonicus) using near infrared spectroscopy combined with chemometric methods.

PubMed

Guo, Xiuhan; Cai, Rui; Wang, Shisheng; Tang, Bo; Li, Yueqing; Zhao, Weijie

2018-01-01

Sea cucumber is the major tonic seafood worldwide, and geographical origin traceability is an important part of its quality and safety control. In this work, a non-destructive method for origin traceability of sea cucumber ( Apostichopus japonicus ) from northern China Sea and East China Sea using near infrared spectroscopy (NIRS) and multivariate analysis methods was proposed. Total fat contents of 189 fresh sea cucumber samples were determined and partial least-squares (PLS) regression was used to establish the quantitative NIRS model. The ordered predictor selection algorithm was performed to select feasible wavelength regions for the construction of PLS and identification models. The identification model was developed by principal component analysis combined with Mahalanobis distance and scaling to the first range algorithms. In the test set of the optimum PLS models, the root mean square error of prediction was 0.45, and correlation coefficient was 0.90. The correct classification rates of 100% were obtained in both identification calibration model and test model. The overall results indicated that NIRS method combined with chemometric analysis was a suitable tool for origin traceability and identification of fresh sea cucumber samples from nine origins in China.

Evaluation of a reflectance model used in the SeaWiFS ocean color algorithm: implications for chlorophyll concentration retrievals

NASA Astrophysics Data System (ADS)

Yan, Banghua; Stamnes, Knut; Toratani, Mitsuhiro; Li, Wei; Stamnes, Jakob J.

2002-10-01

For the atmospheric correction of ocean-color imagery obtained over Case I waters with the Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) instrument the method currently used to relax the black-pixel assumption in the near infrared (NIR) relies on (1) an approximate model for the nadir NIR remote-sensing reflectance and (2) an assumption that the water-leaving radiance is isotropic over the upward hemisphere. Radiance simulations based on a comprehensive radiative-transfer model for the coupled atmosphere-ocean system and measurements of the nadir remote-sensing reflectance at 670 nm compiled in the SeaWiFS Bio-optical Algorithm Mini-Workshop (SeaBAM) database are used to assess the validity of this method. The results show that (1) it is important to improve the flexibility of the reflectance model to provide more realistic predictions of the nadir NIR water-leaving reflectance for different ocean regions and (2) the isotropic assumption should be avoided in the retrieval of ocean color, if the chlorophyll concentration is larger than approximately 6, 10, and 40 mg m-3 when the aerosol optical depth is approximately 0.05, 0.1, and 0.3, respectively. Finally, we extend our scope to Case II ocean waters to gain insight and enhance our understanding of the NIR aspects of ocean color. The results show that the isotropic assumption is invalid in a wider range than in Case I waters owing to the enhanced water-leaving reflectance resulting from oceanic sediments in the NIR wavelengths.

A Retina-Like Dual Band Organic Photosensor Array for Filter-Free Near-Infrared-to-Memory Operations.

PubMed

Wang, Hanlin; Liu, Hongtao; Zhao, Qiang; Ni, Zhenjie; Zou, Ye; Yang, Jie; Wang, Lifeng; Sun, Yanqiu; Guo, Yunlong; Hu, Wenping; Liu, Yunqi

2017-08-01

Human eyes use retina photoreceptor cells to absorb and distinguish photons from different wavelengths to construct an image. Mimicry of such a process and extension of its spectral response into the near-infrared (NIR) is indispensable for night surveillance, retinal prosthetics, and medical imaging applications. Currently, NIR organic photosensors demand optical filters to reduce visible interference, thus making filter-free and anti-visible NIR imaging a challenging task. To solve this limitation, a filter-free and conformal, retina-inspired NIR organic photosensor is presented. Featuring an integration of photosensing and floating-gate memory modules, the device possesses an acute color distinguishing capability. In general, the retina-like photosensor transduces NIR (850 nm) into nonvolatile memory and acts as a dynamic photoswitch under green light (550 nm). In doing this, a filter-free but color-distinguishing photosensor is demonstrated that selectively converts NIR optical signals into nonvolatile memory. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Design of an MR-compatible fNIRS instrument

NASA Astrophysics Data System (ADS)

Emir, Uzay; Ademoglu, Ahmet; Ozturk, Cengizhan; Aydin, Kubilay; Demiralp, Tamer; Kurt, Adnan; Dincer, Alp; Akin, Ata

2005-04-01

Acquiring functional near infrared spectroscopy (fNIRS) and functional magnetic resonance-imaging (fMRI) data are usually done asynchronously. In order to correlate these two different modalities" data, measurements must be performed at the same time. In this study, we have designed a new MR compatible continuous wave intensity based fNIRS device to overcome this problem. For MR compatible fNIRS, we used two LEDs with wavelengths at 660 and 870 nm. There are four photodiodes for light detection. LEDs operated in a sequential multiplexing mode with adjustable "on" time for each LED. Emitted and diffused light was transferred to and from the tissue through 10 m long single mode plastic optical fibers (INDUSTRIAL FIBER OPTICS, INC.). By using fibers, we overcome MR compatibility problems that can be caused by semi-conductors on probe. This MR compatible fNIRS design can provide synchronous measurements with low cost.

Simultaneous multiple wavelength upconversion in a core-shell nanoparticle for enhanced near infrared light harvesting in a dye-sensitized solar cell.

PubMed

Yuan, Chunze; Chen, Guanying; Li, Lin; Damasco, Jossana A; Ning, Zhijun; Xing, Hui; Zhang, Tianmu; Sun, Licheng; Zeng, Hao; Cartwright, Alexander N; Prasad, Paras N; Ågren, Hans

2014-10-22

The efficiency of most photovoltaic devices is severely limited by near-infrared (NIR) transmission losses. To alleviate this limitation, a new type of colloidal upconversion nanoparticles (UCNPs), hexagonal core-shell-structured β-NaYbF4:Er(3+)(2%)/NaYF4:Nd(3+)(30%), is developed and explored in this work as an NIR energy relay material for dye-sensitized solar cells (DSSCs). These UCNPs are able to harvest light energy in multiple NIR regions, and subsequently convert the absorbed energy into visible light where the DSSCs strongly absorb. The NIR-insensitive DSSCs show compelling photocurrent increases through binary upconversion under NIR light illumination either at 785 or 980 nm, substantiating efficient energy relay by these UCNPs. The overall conversion efficiency of the DSSCs was improved with the introduction of UCNPs under simulated AM 1.5 solar irradiation.

Optical changes of dentin in the near-IR as a function of mineral content

NASA Astrophysics Data System (ADS)

Berg, Rhett A.; Simon, Jacob C.; Fried, Daniel; Darling, Cynthia L.

2017-02-01

The optical properties of human dentin can change markedly due to aging, friction from opposing teeth, and acute trauma, resulting in the formation of transparent or sclerotic dentin with increased mineral density. The objective of this study was to determine the optical attenuation coefficient of human dentin tissues with different mineral densities in the near-infrared (NIR) spectral regions from 1300-2200 nm using NIR transillumination and optical coherence tomography (OCT). N=50 dentin samples of varying opacities were obtained by sectioning whole extracted teeth into 150 μm transverse sections at the cemento-enamel junction or the apical root. Transillumination images were acquired with a NIR camera and attenuation measurements were acquired at various NIR wavelengths using a NIR sensitive photodiode. Samples were imaged with transverse microradiography (gold standard) in order to determine the mineral density of each sample.

A portable near-infrared fluorescence image overlay device for surgical navigation (Conference Presentation)

NASA Astrophysics Data System (ADS)

McWade, Melanie A.

2016-03-01

A rise in the use of near-infrared (NIR) fluorescent dyes or intrinsic fluorescent markers for surgical guidance and tissue diagnosis has triggered the development of NIR fluorescence imaging systems. Because NIR wavelengths are invisible to the naked eye, instrumentation must allow surgeons to visualize areas of high fluorescence. Current NIR fluorescence imaging systems have limited ease-of-use because they display fluorescent information on remote display monitors that require surgeons to divert attention away from the patient to identify the location of tissue fluorescence. Furthermore, some systems lack simultaneous visible light imaging which provides valuable spatial context to fluorescence images. We have developed a novel, portable NIR fluorescence imaging approach for intraoperative surgical guidance that provides information for surgical navigation within the clinician's line of sight. The system utilizes a NIR CMOS detector to collect excited NIR fluorescence from the surgical field. Tissues with NIR fluorescence are overlaid with visible light to provide information on tissue margins directly on the surgical field. In vitro studies have shown this versatile imaging system can be applied to applications with both extrinsic NIR contrast agents such as indocyanine green and weaker sources of biological fluorescence such as parathyroid gland tissue. This non-invasive, portable NIR fluorescence imaging system overlays an image directly on tissue, potentially allowing surgical decisions to be made quicker and with greater ease-of-use than current NIR fluorescence imaging systems.

Variables selection methods in near-infrared spectroscopy.

PubMed

Xiaobo, Zou; Jiewen, Zhao; Povey, Malcolm J W; Holmes, Mel; Hanpin, Mao

2010-05-14

Near-infrared (NIR) spectroscopy has increasingly been adopted as an analytical tool in various fields, such as the petrochemical, pharmaceutical, environmental, clinical, agricultural, food and biomedical sectors during the past 15 years. A NIR spectrum of a sample is typically measured by modern scanning instruments at hundreds of equally spaced wavelengths. The large number of spectral variables in most data sets encountered in NIR spectral chemometrics often renders the prediction of a dependent variable unreliable. Recently, considerable effort has been directed towards developing and evaluating different procedures that objectively identify variables which contribute useful information and/or eliminate variables containing mostly noise. This review focuses on the variable selection methods in NIR spectroscopy. Selection methods include some classical approaches, such as manual approach (knowledge based selection), "Univariate" and "Sequential" selection methods; sophisticated methods such as successive projections algorithm (SPA) and uninformative variable elimination (UVE), elaborate search-based strategies such as simulated annealing (SA), artificial neural networks (ANN) and genetic algorithms (GAs) and interval base algorithms such as interval partial least squares (iPLS), windows PLS and iterative PLS. Wavelength selection with B-spline, Kalman filtering, Fisher's weights and Bayesian are also mentioned. Finally, the websites of some variable selection software and toolboxes for non-commercial use are given. Copyright 2010 Elsevier B.V. All rights reserved.

Designing and testing a wearable, wireless fNIRS patch.

PubMed

Abtahi, Mohammadreza; Cay, Gozde; Saikia, Manob Jyoti; Mankodiya, Kunal

2016-08-01

Optical brain monitoring using near infrared (NIR) light has got a lot of attention in order to study the complexity of the brain due to several advantages as oppose to other methods such as EEG, fMRI and PET. There are a few commercially available functional NIR spectroscopy (fNIRS) brain monitoring systems, but they are still non-wearable and pose difficulties in scanning the brain while the participants are in motion. In this work, we present our endeavors to design and test a low-cost, wireless fNIRS patch using NIR light sources at wavelengths of 770 and 830nm, photodetectors and a microcontroller to trigger the light sources, read photodetector's output and transfer data wirelessly (via Bluetooth) to a smart-phone. The patch is essentially a 3-D printed wearable system, recording and displaying the brain hemodynamic responses on smartphone, also eliminates the need for complicated wiring of the electrodes. We have performed rigorous lab experiments on the presented system for its functionality. In a proof of concept experiment, the patch detected the NIR absorption on the arm. Another experiment revealed that the patch's battery could last up to several hours with continuous fNIRS recording with and without wireless data transfer.

Hyperspectral imaging with near-infrared-enabled mobile phones for tissue oximetry

NASA Astrophysics Data System (ADS)

Lin, Jonathan L.; Ghassemi, Pejhman; Chen, Yu; Pfefer, Joshua

2018-02-01

Hyperspectral reflectance imaging (HRI) is an emerging clinical tool for characterizing spatial and temporal variations in blood perfusion and oxygenation for applications such as burn assessment, wound healing, retinal exams and intraoperative tissue viability assessment. Since clinical HRI-based oximeters often use near-infrared (NIR) light, NIR-enabled mobile phones may provide a useful platform for future point-of-care devices. Furthermore, quantitative NIR imaging on mobile phones may dramatically increase the availability and accessibility of medical diagnostics for low-resource settings. We have evaluated the potential for phone-based NIR oximetry imaging and elucidated factors affecting performance using devices from two different manufacturers, as well as a scientific CCD. A broadband light source and liquid crystal tunable filter were used for imaging at 10 nm bands from 650 to 1000 nm. Spectral sensitivity measurements indicated that mobile phones with standard NIR blocking filters had minimal response beyond 700 nm, whereas one modified phone showed sensitivity to 800 nm and another to 1000 nm. Red pixel channels showed the greatest sensitivity up to 800 nm, whereas all channels provided essentially equivalent sensitivity at longer wavelengths. Referencing of blood oxygenation levels was performed with a CO-oximeter. HRI measurements were performed using cuvettes filled with hemoglobin solutions of different oxygen saturation levels. Good agreement between absorbance spectra measured with mobile phone and a CCD cameras were seen for wavelengths below 900 nm. Saturation estimates showed root-mean-squared-errors of 5.2% and 4.5% for the CCD and phone, respectively. Overall, this work provides strong evidence of the potential for mobile phones to provide quantitative spectral imaging in the NIR for applications such as oximetry, and generates practical insights into factors that impact performance as well as test methods for performance assessment.

Laser-induced fine structures on silicon exposed to THz-FEL

NASA Astrophysics Data System (ADS)

Irizawa, Akinori; Suga, Shigemasa; Nagashima, Takeshi; Higashiya, Atsushi; Hashida, Masaki; Sakabe, Shuji

2017-12-01

We found the irradiation of focused linearly polarized terahertz (THz)-waves emitted from THz free-electron laser (THz-FEL) engraved fine periodic stripe structures on the surfaces of single-crystal Si wafers. The experiments were performed at several wavelengths ranging from 50 to 82 μm with a macro-pulse fluence up to 32 J/cm2. The engraved structures are considered equivalent to the laser-induced periodic surface structures (LIPSS) produced by the irradiation of a femtosecond (fs)-pulsed laser in the near-infrared (NIR) region. However, the minimum period of ˜1/25 of the wavelength in the present case of THz-FEL is surely much smaller than those reported so far by use of fs-lasers and no more explicable by the so far proposed mechanisms. The finer LIPSS confirmed by longer-wavelength laser excitation by means of THz-FEL motivates investigation into the universal mechanism of LIPSS formation, which has been under a hot debate for decades.

Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Xiang; Zhang, Yuanwei; Takle, Kendra

2016-01-26

Near Infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) have recently been proposed in order to broaden the absorption range and to boost upconversion efficiency. However, implementing this strategy has been limited only to bare core UCNP structures that are faintly luminescent. Herein, we report on an approach to achieve significantly enhanced upconversion luminescence in dye-sensitized core-active shell UCNPs with a broadened absorption range via the doping of ytterbium ions in the UCNP shell in order to bridge the energy transfer from the dye to the UCNP core. As a result, we have been able to synergize the two most practical upconversionmore » booster effectors (dye-sensitizing and core/shell enhancement). The absolute quantum yield of our dye-sensitized core/active shell UCNPs at 800 nm was determined to be ~6% at 2 W/cm2, about 33 times larger than the highest value reported to date for existing 800 nm excitable UCNPs. Moreover, for the first time, by using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a wavelength that is compatible with deep tissue penetrable near the infrared wavelength at 800 nm. Finally, amphiphilic triblock copolymer, Pluronic F127 coatings permit the transfer of hydrophobic UCNPs into water, resulting in water-soluble nanoparticles with well-preserved optical property in aqueous solution. We believe that this research offers a new solution to enhance upconversion efficiency for photonic and biophotonic purposes and opens up new opportunities to use UCNPs as a NIR relay for optogenetic applications.« less

Normal Raman and surface enhanced Raman spectroscopic experiments with thin layer chromatography spots of essential amino acids using different laser excitation sources.

PubMed

István, Krisztina; Keresztury, Gábor; Szép, Andrea

2003-06-01

A comparative study of the feasibility and efficiency of Raman spectroscopic detection of thin layer chromatography (TLC) spots of some weak Raman scatterers (essential amino acids, namely, glycine and L-forms of alanine, serine, valine, proline, hydroxyproline, and phenylalanine) was carried out using four different visible and near-infrared (NIR) laser radiations with wavelengths of 532, 633, 785, and 1064 nm. Three types of commercial TLC plates were tested and the possibility of inducing surface enhanced Raman scattering (SERS) by means of Ag-sol was also investigated. The spectra obtained from spotted analytes adsorbed on TLC plates were of very different quality strongly depending on the excitation wavelength, the wetness of the samples, and the compounds examined. The best results were obtained with the simple silica TLC plate, and it has been established that the longest wavelength (lowest energy) NIR excitation of a Nd:YAG laser is definitely more suitable for generating normal Raman scattering of analyte spots than any of the visible radiations. Concerning SERS with application of Ag-sol to the TLC spots, 1-3 orders of magnitude enhancement was observed with wet samples, the greatest with the 532 nm radiation and gradually smaller with the longer wavelength excitations. It is shown, however, that due to severe adsorption-induced spectral distortions and increased sensitivity to microscopic inhomogeneity of the sample, none of the SERS spectra obtained with the dispersive Raman microscope operating in the visible region were superior to the best NIR normal FT-Raman spectra, as far as sample identification is concerned.

Normal Raman and surface enhanced Raman spectroscopic experiments with thin layer chromatography spots of essential amino acids using different laser excitation sources

NASA Astrophysics Data System (ADS)

István, Krisztina; Keresztury, Gábor; Szép, Andrea

2003-06-01

A comparative study of the feasibility and efficiency of Raman spectroscopic detection of thin layer chromatography (TLC) spots of some weak Raman scatterers (essential amino acids, namely, glycine and L-forms of alanine, serine, valine, proline, hydroxyproline, and phenylalanine) was carried out using four different visible and near-infrared (NIR) laser radiations with wavelengths of 532, 633, 785, and 1064 nm. Three types of commercial TLC plates were tested and the possibility of inducing surface enhanced Raman scattering (SERS) by means of Ag-sol was also investigated. The spectra obtained from spotted analytes adsorbed on TLC plates were of very different quality strongly depending on the excitation wavelength, the wetness of the samples, and the compounds examined. The best results were obtained with the simple silica TLC plate, and it has been established that the longest wavelength (lowest energy) NIR excitation of a Nd:YAG laser is definitely more suitable for generating normal Raman scattering of analyte spots than any of the visible radiations. Concerning SERS with application of Ag-sol to the TLC spots, 1-3 orders of magnitude enhancement was observed with wet samples, the greatest with the 532 nm radiation and gradually smaller with the longer wavelength excitations. It is shown, however, that due to severe adsorption-induced spectral distortions and increased sensitivity to microscopic inhomogeneity of the sample, none of the SERS spectra obtained with the dispersive Raman microscope operating in the visible region were superior to the best NIR normal FT-Raman spectra, as far as sample identification is concerned.

Absolute extinction and the influence of environment - Dark cloud sight lines toward VCT 10, 30, and Walker 67

NASA Technical Reports Server (NTRS)

Cardelli, Jason A.; Clayton, Geoffrey C.

1991-01-01

The range of validity of the average absolute extinction law (AAEL) proposed by Cardelli et al. (1988 and 1989) is investigated, combining published visible and NIR data with IUE UV observations for three lines of sight through dense dark cloud environments with high values of total-to-selective extinction. The characteristics of the data sets and the reduction and parameterization methods applied are described in detail, and the results are presented in extensive tables and graphs. Good agreement with the AAEL is demonstrated for wavelengths from 3.4 microns to 250 nm, but significant deviations are found at shorter wavelengths (where previous studies of lines of sight through bright nebulosity found good agreement with the AAEL). These differences are attributed to the effects of coatings on small-bump and FUV grains.

A new Cassegrain calibration lamp unit for the Blanco Telescope

NASA Astrophysics Data System (ADS)

Points, S. D.; James, D. J.; Tighe, R.; Montané, A.; David, N.; Martínez, M.

2016-08-01

The f/8 RC-Cassegrain Focus of the Blanco Telescope at Cerro Tololo Inter-American Observatory, hosts two new instruments: COSMOS, a multi-object spectrograph in the visible wavelength range (350 - 1030nm), and ARCoIRIS, a NIR cross-dispersed spectrograph featuring 6 spectral orders spanning 0.8 - 2.45μm. Here we describe a calibration lamp unit designed to deliver the required illumination at the telescope focal plane for both instruments. These requirements are: (1) an f/8 beam of light covering a spot of 92mm diameter (or 10 arcmin) for a wavelength range of 0.35μm through 2.5μm and (2) no saturation of flat-field calibrations for the minimal exposure times permitted by each instrument, and (3) few saturated spectral lines when using the wavelength calibration lamps for the instruments. To meet these requirements this unit contains an adjustable quartz halogen lamp for flat-field calibrations, and one hollow cathode lamp and four penray lamps for wavelength calibrations. The wavelength calibration lamps are selected to provide optimal spectral coverage for the instrument mounted and can be used individually or in sets. The device designed is based on an 8-inch diameter integrating sphere, the output of which is optimized to match the f/8 calibration input delivery system which is a refractive system based on fused-silica lenses. We describe the optical design, the opto-mechanical design, the electronic control and give results of the performance of the system.

TROPOMI, the Sentinel 5 precursor instrument for air quality and climate observations: status of the current design

NASA Astrophysics Data System (ADS)

Voors, Robert; de Vries, Johan; Bhatti, Ianjit S.; Lobb, Dan; Wood, Trevor; van der Valk, Nick; Aben, Ilse; Veefkind, Pepijn

2017-11-01

TROPOMI, the Tropospheric Monitoring Instrument, is a passive UV-VIS-NIR-SWIR trace gas spectrograph in the line of SCIAMACHY (2002) and OMI (2004), instruments with the Netherlands in a leading role. Both instruments are very successful and remained operational long after their nominal life time. TROPOMI is the next step, scheduled for launch in 2015. It combines the broad wavelength range from SCIAMACHY from UV to SWIR and the broad viewing angle push-broom concept from OMI, which makes daily global coverage in combination with good spatial resolution possible. Using spectral bands from 270-500nm (UV-VIS) 675-775nm (NIR) and 2305-2385nm (SWIR) at moderate resolution (0.25 to 0.6nm) TROPOMI will measure O3, NO2, SO2, BrO, HCHO and H2O tropospheric columns from the UV-VIS-NIR wavelength range and CO and CH4 tropospheric columns from the SWIR wavelength range. Cloud information will be derived primarily from the O2A band in the NIR. This will help, together with the aerosol information, in constraining the light path of backscattered solar radiation. Methane (CH4), CO2 and Carbon monoxide (CO) are the key gases of the global carbon cycle. Of these, Methane is by far the least understood in terms of its sources and is most difficult to predict its future trend. Global space observations are needed to inform atmospheric models. The SWIR channel of TROPOMI is designed to achieve the spectral, spatial and SNR resolution required for this task. TROPOMI will yield an improved accuracy of the tropospheric products compared to the instruments currently in orbit. TROPOMI will take a major step forward in spatial resolution and sensitivity. The nominal observations are at 7 x 7 km2 at nadir and the signal-to-noises are sufficient for trace gas retrieval even at very low albedos (down to 2%). This spatial resolution allows observation of air quality at sub-city level and the high signal-to-noises means that the instrument can perform useful measurements in the darkest conditions. TROPOMI is currently in its detailed design phase. This paper gives an overview of the challenges and current performances. From unit level engineering models first results are becoming available. Early results are promising and this paper discusses some of these early H/W results. TROPOMI is the single payload on the Sentinel-5 precursor mission which is a joint initiative of the European Community (EC) and of the European Space Agency (ESA). The 2015 launch intends to bridge the data stream from OMI / SCIAMACHY and the upcoming Sentinel 5 mission. The instrument is funded jointly by the Netherlands Space Office and by ESA. Dutch Space is the instrument prime contractor. SSTL in the UK is developing the SWIR module with a significant contribution from SRON. Dutch Space and TNO are working as an integrated team for the UVN module. KNMI and SRON are responsible for ensuring the scientific capabilities of the instrument.

Multispectral laser imaging for advanced food analysis

NASA Astrophysics Data System (ADS)

Senni, L.; Burrascano, P.; Ricci, M.

2016-07-01

A hardware-software apparatus for food inspection capable of realizing multispectral NIR laser imaging at four different wavelengths is herein discussed. The system was designed to operate in a through-transmission configuration to detect the presence of unwanted foreign bodies inside samples, whether packed or unpacked. A modified Lock-In technique was employed to counterbalance the significant signal intensity attenuation due to transmission across the sample and to extract the multispectral information more efficiently. The NIR laser wavelengths used to acquire the multispectral images can be varied to deal with different materials and to focus on specific aspects. In the present work the wavelengths were selected after a preliminary analysis to enhance the image contrast between foreign bodies and food in the sample, thus identifying the location and nature of the defects. Experimental results obtained from several specimens, with and without packaging, are presented and the multispectral image processing as well as the achievable spatial resolution of the system are discussed.

Screening analysis of biodiesel feedstock using UV-vis, NIR and synchronous fluorescence spectrometries and the successive projections algorithm.

PubMed

Insausti, Matías; Gomes, Adriano A; Cruz, Fernanda V; Pistonesi, Marcelo F; Araujo, Mario C U; Galvão, Roberto K H; Pereira, Claudete F; Band, Beatriz S F

2012-08-15

This paper investigates the use of UV-vis, near infrared (NIR) and synchronous fluorescence (SF) spectrometries coupled with multivariate classification methods to discriminate biodiesel samples with respect to the base oil employed in their production. More specifically, the present work extends previous studies by investigating the discrimination of corn-based biodiesel from two other biodiesel types (sunflower and soybean). Two classification methods are compared, namely full-spectrum SIMCA (soft independent modelling of class analogies) and SPA-LDA (linear discriminant analysis with variables selected by the successive projections algorithm). Regardless of the spectrometric technique employed, full-spectrum SIMCA did not provide an appropriate discrimination of the three biodiesel types. In contrast, all samples were correctly classified on the basis of a reduced number of wavelengths selected by SPA-LDA. It can be concluded that UV-vis, NIR and SF spectrometries can be successfully employed to discriminate corn-based biodiesel from the two other biodiesel types, but wavelength selection by SPA-LDA is key to the proper separation of the classes. Copyright © 2012 Elsevier B.V. All rights reserved.

Remote sensing and the optical properties of the narrow cylindrical leaves of Juncus roemerianus

USGS Publications Warehouse

Ramsey, Elijah W.; Rangoonwala, A.

2004-01-01

To develop a more complete foundation for remote sensing of the marsh grass Juncus roemerianus, we measured the optical properties of its cylindrical leaves at sites of different canopy height, biomass composition and amount, and connectivity to ocean flushing. To measure the leaf optical properties, we adapted a technique used for conifer needles. After establishing the reliability and limits of the adapted technique to the wider J.roemerianus leaves, mean transmittance and reflectance spectra were compared to associated leaf diameters from two dates in 1999 and 2002 and at each site. Transmittance was inversely related to leaf diameter. Mean transmittance and reflectance generated from reoccupation of many field sites in 2002 indicated little or no difference in transmittance between years, a slight reflectance difference in the visible (<2%) and a slightly higher reflectance difference in the near infrared (NIR) (<4%). Site comparison indicated limited ability to separate leaf transmittance but not reflectance by marsh type (e.g., low, medium, high) or biomass. Excluding one outlier, we found leaf transmittances could be adequately represented as 1% ?? 0.2% in the visible and 9% ?? 1% in the NIR and leaf reflectances represented from 14% to 16% in the visible and 71% to 75% in the NIR (the reflectance ranges represent 1999 and 2002 means). Reflectance and transmittance spectra associated with the dead J. roemerianus leaves displayed a spectrally flat increase from the visible to the NIR wavelengths. In total, we documented the atypical optical properties of the cylindrical J. roemerianus leaves and showed that to a first approximation, single means could represent leaf transmittance and visible leaf reflectance across all marsh zones and, after accounting for sample standardization, possibly the NIR reflectance as well.

SIGNATURES OF GRAVITATIONAL INSTABILITY IN RESOLVED IMAGES OF PROTOSTELLAR DISKS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dong, Ruobing; Vorobyov, Eduard; Pavlyuchenkov, Yaroslav

2016-06-01

Protostellar (class 0/I) disks, which have masses comparable to those of their nascent host stars and are fed continuously from their natal infalling envelopes, are prone to gravitational instability (GI). Motivated by advances in near-infrared (NIR) adaptive optics imaging and millimeter-wave interferometry, we explore the observational signatures of GI in disks using hydrodynamical and Monte Carlo radiative transfer simulations to synthesize NIR scattered light images and millimeter dust continuum maps. Spiral arms induced by GI, located at disk radii of hundreds of astronomical units, are local overdensities and have their photospheres displaced to higher altitudes above the disk midplane; therefore,more » arms scatter more NIR light from their central stars than inter-arm regions, and are detectable at distances up to 1 kpc by Gemini/GPI, VLT/SPHERE, and Subaru/HiCIAO/SCExAO. In contrast, collapsed clumps formed by disk fragmentation have such strong local gravitational fields that their scattering photospheres are at lower altitudes; such fragments appear fainter than their surroundings in NIR scattered light. Spiral arms and streamers recently imaged in four FU Ori systems at NIR wavelengths resemble GI-induced structures and support the interpretation that FUors are gravitationally unstable protostellar disks. At millimeter wavelengths, both spirals and clumps appear brighter in thermal emission than the ambient disk and can be detected by ALMA at distances up to 0.4 kpc with one hour integration times at ∼0.″1 resolution. Collapsed fragments having masses ≳1 M {sub J} can be detected by ALMA within ∼10 minutes.« less

CROSS-CORRELATION OF NEAR- AND FAR-INFRARED BACKGROUND ANISOTROPIES AS TRACED BY SPITZER AND HERSCHEL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thacker, Cameron; Gong, Yan; Cooray, Asantha

We present the cross-correlation between the far-infrared (far-IR) background fluctuations as measured with the Herschel Space Observatory at 250, 350, and 500 μm and the near-infrared (near-IR) background fluctuations with the Spitzer Space Telescope at 3.6 and 4.5 μm. The cross-correlation between the FIR and NIR background anisotropies is detected such that the correlation coefficient at a few to 10 arcminute angular scale decreases from 0.3 to 0.1 when the FIR wavelength increases from 250 to 500 μm. We model the cross-correlation using a halo model with three components: (a) FIR bright or dusty star-forming galaxies below the masking depth inmore » Herschel maps, (b) NIR faint galaxies below the masking depth, and (c) intra-halo light (IHL), or diffuse stars in dark matter halos, that is likely dominating the large-scale NIR fluctuations. The model is able to reasonably reproduce the auto-correlations at each of the FIR wavelengths and at 3.6 μm and their corresponding cross-correlations. While the FIR and NIR auto-correlations are dominated by faint, dusty, star-forming galaxies and IHL, respectively, we find that roughly half of the cross-correlation between the NIR and FIR backgrounds is due to the same dusty galaxies that remain unmasked at 3.6 μm. The remaining signal in the cross-correlation is due to IHL present in the same dark matter halos as those hosting the same faint and unmasked galaxies.« less

Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 μm

DOE PAGES

Amanullah, R.; Johansson, J.; Goobar, A.; ...

2015-09-07

Here, we present ultraviolet (UV) observations of six nearby Type Ia supernovae (SNe Ia) obtained with the Hubble Space Telescope, three of which were also observed in the near-IR (NIR) with Wide-Field Camera 3. UV observations with the Swift satellite, as well as ground-based optical and NIR data provide complementary information. The combined data set covers the wavelength range 0.2–2 μm. By also including archival data of SN 2014J, we analyse a sample spanning observed colour excesses up to E(B - V) = 1.4 mag. We study the wavelength-dependent extinction of each individual SN and find a diversity of reddeningmore » laws when characterized by the total-to-selective extinction R V. In particular, we note that for the two SNe with E(B - V) ≳1 mag, for which the colour excess is dominated by dust extinction, we find R V = 1.4 ± 0.1 and R V = 2.8 ± 0.1. Adding UV photometry reduces the uncertainty of fitted R V by ~50 percent allowing us to also measure R V of individual low-extinction objects which point to a similar diversity, currently not accounted for in the analyses when SNe Ia are used for studying the expansion history of the Universe.« less

Determination of total flavonoids content in fresh Ginkgo biloba leaf with different colors using near infrared spectroscopy

NASA Astrophysics Data System (ADS)

Shi, Ji-yong; Zou, Xiao-bo; Zhao, Jie-wen; Mel, Holmes; Wang, Kai-liang; Wang, Xue; Chen, Hong

Total flavonoids content is often considered an important quality index of Ginkgo biloba leaf. The feasibility of using near infrared (NIR) spectra at the wavelength range of 10,000-4000 cm-1 for rapid and nondestructive determination of total flavonoids content in G. biloba leaf was investigated. 120 fresh G. biloba leaves in different colors (green, green-yellowish and yellow) were used to spectra acquisition and total flavonoids determination. Partial least squares (PLS), interval partial least squares (iPLS) and synergy interval partial least squares (SiPLS) were used to develop calibration models for total flavonoids content in two colors leaves (green-yellowish and yellow) and three colors leaves (green, green-yellowish and yellow), respectively. The level of total flavonoids content for green, green-yellowish and yellow leaves was in an increasing order. Two characteristic wavelength regions (5840-6090 cm-1 and 6620-6880 cm-1), which corresponded to the absorptions of two aromatic rings in basic flavonoid structure, were selected by SiPLS. The optimal SiPLS model for total flavonoids content in the two colors leaves (r2 = 0.82, RMSEP = 2.62 mg g-1) had better performance than PLS and iPLS models. It could be concluded that NIR spectroscopy has significant potential in the nondestructive determination of total flavonoids content in fresh G. biloba leaf.

[Study on rapid determination and analysis of rocket kerosene by near infrared spectrum and chemometrics].

PubMed

Xia, Ben-Li; Cong, Ji-Xin; Li, Xia; Wang, Xuan-Jun

2011-06-01

The rocket kerosene quality properties such as density, distillation range, viscosity and iodine value were successfully measured based on their near-infrared spectrum (NIRS) and chemometrics. In the present paper, more than 70 rocket kerosene samples were determined by near infrared spectrum, the models were built using the partial least squares method within the appropriate wavelength range. The correlation coefficients (R2) of every rocket kerosene's quality properties ranged from 0.862 to 0.999. Ten unknown samples were determined with the model, and the result showed that the prediction accuracy of near infrared spectrum method accords with standard analysis requirements. The new method is well suitable for replacing the traditional standard method to rapidly determine the properties of the rocket kerosene.

Singlet-Oxygen Generation From Individual Semiconducting and Metallic Nanostructures During Near-Infrared Laser Trapping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, Bennett E.; Roder, Paden B.; Hanson, Jennifer L.

2015-03-13

Photodynamic therapy has been used for several decades in the treatment of solid tumors through the generation of reactive singlet-oxygen species (1O2). Recently, nanoscale metallic and semiconducting materials have been reported to act as photosensitizing agents with additional diagnostic and therapeutic functionality. To date there have been no reports of observing the generation of singlet-oxygen at the level of single nanostructures, particularly at near infrared (NIR) wavelengths. Here we demonstrate that NIR laser-tweezers can be used to observe the formation of singlet-oxygen produced from individual silicon and gold nanowires via use of a commercially available reporting dye. The laser trapmore » also induces 2-photon photoexcitation of the dye following a chemical reaction with singlet oxygen. Corresponding 2-photon emission spectra confirms the generation of singlet oxygen from individual silicon nanowires at room temperature (30°C), suggesting a range of applications in understanding the impact of 1O2 on individual cancer cells.« less

[Determination of process variable pH in solid-state fermentation by FT-NIR spectroscopy and extreme learning machine (ELM)].

PubMed

Liu, Guo-hai; Jiang, Hui; Xiao, Xia-hong; Zhang, Dong-juan; Mei, Cong-li; Ding, Yu-han

2012-04-01

Fourier transform near-infrared (FT-NIR) spectroscopy was attempted to determine pH, which is one of the key process parameters in solid-state fermentation of crop straws. First, near infrared spectra of 140 solid-state fermented product samples were obtained by near infrared spectroscopy system in the wavelength range of 10 000-4 000 cm(-1), and then the reference measurement results of pH were achieved by pH meter. Thereafter, the extreme learning machine (ELM) was employed to calibrate model. In the calibration model, the optimal number of PCs and the optimal number of hidden-layer nodes of ELM network were determined by the cross-validation. Experimental results showed that the optimal ELM model was achieved with 1040-1 topology construction as follows: R(p) = 0.961 8 and RMSEP = 0.104 4 in the prediction set. The research achievement could provide technological basis for the on-line measurement of the process parameters in solid-state fermentation.

In vivo near-IR imaging of approximal dental decay at 1,310 nm.

PubMed

Staninec, Michal; Lee, Chulsung; Darling, Cynthia L; Fried, Daniel

2010-04-01

The high transparency of dental enamel in the near-IR (NIR) light at 1,310-nm can be exploited for imaging dental caries without the use of ionizing radiation (X-rays). We present the results of the first in vivo imaging study in which NIR images were acquired of approximal contact surfaces. NIR imaging hand-pieces were developed and attached to a compact InGaAs focal plane array and subsequently used to acquire in vivo NIR images of 33 caries lesions on 18 test subjects. The carious lesions were discernible on bitewing radiographs, but were not visible upon clinical examination. NIR images were acquired in vivo from three directions and the majority of lesions examined were too small to require restoration, based on accepted bitewing radiograph criteria. All but one of the 33 lesions examined were successfully imaged from at least one direction. This first in vivo study of imaging at the 1,310-nm wavelength region shows that NIR imaging has great potential as a screening tool for the detection of approximal lesions without the use of ionizing radiation. (c) 2010 Wiley-Liss, Inc.

Multimodal Imaging of Disease-Associated Pigmentary Changes in Retinitis Pigmentosa

PubMed Central

Schuerch, Kaspar; Marsiglia, Marcela; Lee, Winston; Tsang, Stephen H.; Sparrow, Janet R.

2016-01-01

Purpose Using multiple imaging modalities we evaluated the changes in photoreceptor cells and RPE that are associated with bone spicule-shaped melanin pigmentation in retinitis pigmentosa (RP). Methods In a cohort of 60 RP patients, short-wavelength autofluorescence (SW-AF), near-infrared (NIR)-AF, NIR-reflectance (NIR-R), spectral domain optical coherence tomography (SD-OCT) and color fundus images were studied. Results Central AF rings were visible in both SW-AF and NIR-AF images. Bone spicule pigmentation was non-reflective in NIR-R, hypoautofluorescent with SW-AF and NIR-AF imaging and presented as intraretinal hyperreflective foci in SD-OCT images. In areas beyond the AF ring outer border, the photoreceptor ellipsoid zone (EZ) band was absent in SD-OCT scans and the visibility of choroidal vessels in SW-AF, NIR-AF and NIR-R images was indicative of reduced RPE pigmentation. Choroidal visibility was most pronounced in the zone approaching peripheral areas of bone spicule pigmentation; here RPE/Bruch’s membrane thinning became apparent in SD-OCT scans. Conclusions These findings are consistent with a process by which RPE cells vacate their monolayer and migrate into inner retina in response to photoreceptor cell degeneration. The remaining RPE spread, undergo thinning and consequently become less pigmented. An explanation for the absence of NIR-AF melanin signal in relation to bone spicule pigmentation is not forthcoming. PMID:28005673

Investigating the use of Egyptian blue in Roman Egyptian portraits and panels from Tebtunis, Egypt

NASA Astrophysics Data System (ADS)

Ganio, Monica; Salvant, Johanna; Williams, Jane; Lee, Lynn; Cossairt, Oliver; Walton, Marc

2015-11-01

The use of the pigment Egyptian blue is investigated on a corpus of fifteen mummy portraits and Roman-period paintings from Tebtunis, Egypt, housed in the Phoebe A. Hearst Museum of Anthropology at the University of California, Berkeley. Egyptian blue has a strong luminescence response in the near infrared that can be exploited to created wide-field images noninvasively showing the distribution of the pigment on a work of art. A growing body of publications in the last decade highlights the increasing use of this tool and its sensitive detection limits. However, the technique is not wavelength specific. Both excitation and emission occur in a broad range. Although Egyptian blue has a strong emission in the NIR, a myriad of other compounds may emit light in this spectral region when excited in the visible. The limited number of studies including complementary analysis to verify the presence of Egyptian blue does not allow its identification on the basis of NIR luminescence alone. Through the use of in situ X-ray fluorescence and X-ray diffraction, and scanning electron microscopy/energy-dispersive spectroscopy of cross sections, this paper confirms the identification of Egyptian blue by NIR luminescence in unexpected areas, i.e., those not blue in appearance.

Monitoring of itaconic acid hydrogenation in a trickle bed reactor using fiber-optic coupled near-infrared spectroscopy.

PubMed

Wood, Joseph; Turner, Paul H

2003-03-01

Near-infrared (NIR) spectroscopy has been applied to determine the conversion of itaconic acid in the effluent stream of a trickle bed reactor. Hydrogenation of itaconic to methyl succinic acid was carried out, with the trickle bed operating in recycle mode. For the first time, NIR spectra of itaconic and methyl succinic acids in aqueous solution, and aqueous mixtures withdrawn from the reactor over a range of reaction times, have been recorded using a fiberoptic sampling probe. The infrared spectra displayed a clear isolated absorption band at a wavenumber of 6186 cm(-1) (wavelength 1.617 microm) resulting from the =C-H bonds of itaconic acid, which was found to decrease in intensity with increasing reaction time. The feature could be more clearly observed from plots of the first derivatives of the spectra. A partial least-squares (PLS) model was developed from the spectra of 13 reference samples and was used successfully to calculate the concentration of the two acids in the reactor effluent solution. Itaconic acid conversions of 23-29% were calculated after 360 min of reaction time. The potential of FT-NIR with fiber-optic sampling for remote monitoring of three-phase catalytic reactors and validation of catalytic reactor models is highlighted in the paper.

In vivo measurements of the influence of the skin on cerebral oxygenation changes measured with near-infrared spectrophotometry (NIRS)

NASA Astrophysics Data System (ADS)

Klaessens, John H. G. M.; van Os, Sandra H. G.; Hopman, Jeroen C. W.; Liem, K. D.; van de Bor, Margot; Thijssen, Johan M.

2004-07-01

Goal: To investigate the influence of skin on the accuracy and precision of regional cerebral oxygenation measurements using CW-NIRS and to reduce the inter individual variability of NIRS measurements by normalization with data from an extra wavelength. Method: Three piglets (7.8-9.3 kg) were anesthetized, paralyzed and mechanically ventilated. Receiving optodes were placed over the left and right hemisphere (C3, C4 EEG placement code) and one emitting optode on Cz position (optode distance=1.8cm). Optical densities (OD) were measured for 3 wavelengths (767, 850, 905 nm) (OXYMON) during stable normoxic, mild and deep hypoxemic conditions (SaO2=100%, 80% and 60%) of one minute in each region. This was repeated 3 times: all optodes with skin (condition 1); one receiving optode directly on the skull (2); emitting and also receiving optode on the skull (3). The absolute cO2Hb, cHHb, ctHb concentrations (μmol/L) were calculated from the OD's and changes with respect to the SaO2=100% condition were estimated. Because ODs varied over a large range, the light intensity was externally attenuated to adapt to the range of the spectrophotometer. The data were then corrected for these attenuation effects and for pathlength changes caused by skin removal using the OD at the independent wavelength (λ=975nm). Results: Removal of the skin resulted in an increase of the absorption values (average 0.25 OD in condition 2 and 0.42 OD in condition 3 with respect to condition 1). The change from normoxic to medium, and to deep hypoxic conditions produced a decrease of cO2Hb (-15, and -29 μmol/L, respectively), an increase in cHHb (+16, and +35 μmol/L) and in ctHb (+1, and +5 μmol/L). Total skin removal yielded an extra change in cO2Hb (-5, -1 μmol/L), cHHb (+8, +9 μmol/L), and ctHb (+3, +8 μmol /L). The coefficient of variability of the absolute concentration changes was considerably decreased by the normalization of densities by the density obtained at 795 nm. Conclusion: Skin and subcutaneous layers influence the regional oxygenation measurements but the estimated concentration changes are dominated by changes of the oxygenation levels in the brain. Inter individual variability can be considerably reduced by the normalization.

Nanostructured pyronin Y thin films as a new organic semiconductor: Linear/nonlinear optics, band gap and dielectric properties

NASA Astrophysics Data System (ADS)

Zahran, H. Y.; Yahia, I. S.; Alamri, F. H.

2017-05-01

Pyronin Y dye (PY) is a kind of xanthene derivatives. Thin films of pyronin Y were deposited onto highly cleaned glass substrates using low-cost/spin coating technique. The structure properties of pyronin Y thin films with different thicknesses were investigated by using X-ray diffraction (XRD) and atomic force microscope (AFM). PY thin films for all the studied thicknesses have an amorphous structure supporting the short range order of the grain size. AFM supports the nanostructure with spherical/clusters morphologies of the investigated thin films. The optical constants of pyronin Y thin films for various thicknesses were studied by using UV-vis-NIR spectrophotometer in the wavelength range 350-2500 nm. The transmittance T(λ), reflectance R(λ) spectral and absorbance (abs(λ)) were obtained for all film thicknesses at room temperature and the normal light incident. These films showed a high transmittance in the wide scale wavelengths. For different thicknesses of the studied thin films, the optical band gaps were determined and their values around 2 eV. Real and imaginary dielectric constants, dissipation factor and the nonlinear optical parameters were calculated in the wavelengths to the range 300-2500 nm. The pyronin Y is a new organic semiconductor with a good optical absorption in UV-vis regions and it is suitable for nonlinear optical applications.

Reversible near-infrared light directed reflection in a self-organized helical superstructure loaded with upconversion nanoparticles.

PubMed

Wang, Ling; Dong, Hao; Li, Yannian; Xue, Chenming; Sun, Ling-Dong; Yan, Chun-Hua; Li, Quan

2014-03-26

Adding external, dynamic control to self-organized superstructures with desired functionalities is an important leap necessary in leveraging the fascinating molecular systems for applications. Here, the new light-driven chiral molecular switch and upconversion nanoparticles, doped in a liquid crystal media, were able to self-organize into an optically tunable helical superstructure. The resulting nanoparticle impregnated helical superstructure was found to exhibit unprecedented reversible near-infrared (NIR) light-guided tunable behavior only by modulating the excitation power density of a continuous-wave NIR laser (980 nm). Upon irradiation by the NIR laser at the high power density, the reflection wavelength of the photonic superstructure red-shifted, whereas its reverse process occurred upon irradiation by the same laser but with the lower power density. Furthermore, reversible dynamic NIR-light-driven red, green, and blue reflections in a single thin film, achieved only by varying the power density of the NIR light, were for the first time demonstrated.

Caries detection and diagnostics with near-infrared light transillumination: clinical experiences.

PubMed

Söchtig, Friederike; Hickel, Reinhard; Kühnisch, Jan

2014-06-01

The aim of this paper was to present the function and potential of diagnosing caries lesions using a recently introduced near-infrared (NIR) transillumination technique (DIAGNOcam, KaVo). The study included 130 adolescents and adults with complete permanent dentition (age > 12). All patients underwent visual examination and, if necessary, bitewing radiographs. Proximal and occlusal surfaces, which had not yet been restored, were photographed by a NIR transillumination camera system using light with a wavelength of 780 nm rather than ionizing radiation. Of the study patients, 85 showed 127 proximal dentin caries lesions that were treated operatively. A cross table shows the correlation of radiography and NIR transillumination. Based on our practical clinical experiences to date, a possible classifi cation of diagnosis is introduced. The main result of our study was that NIR light was able to visualize caries lesions on proximal and occlusal surfaces. The study suggests that NIR transillumination is a method that may help to avoid bitewing radiographs for diagnosis of caries in everyday clinical practice.

Surface plasmon resonance near-infrared spectroscopy.

PubMed

Ikehata, Akifumi; Itoh, Tamitake; Ozaki, Yukihiro

2004-11-01

Near-infrared (NIR) spectroscopy is ill-suited to microanalysis because of its low absorptivity. We have developed a highly sensitive detection method for NIR spectroscopy based on absorption-sensitive surface plasmon resonance (SPR). The newly named SPR-NIR spectroscopy, which may open the way for NIR spectroscopy in microanalysis and surface science, is realized by an attachment of the Kretschmann configuration equipped with a mechanism for fine angular adjustment of incident light. The angular sweep of incident light enables us to make a tuning of a SPR peak for an absorption band of sample medium. From the dependences of wavelength, incident angle, and thickness of a gold film on the intensity of the SPR peak, it has been found that the absorbance can be enhanced by approximately 100 times compared with the absorbance obtained without the gold film under optimum conditions. This article reports the details of the experimental setup and the characteristics of absorption-sensitive SPR in the NIR region, together with some experimental results obtained by using it.

Integration of multispectral satellite and hyperspectral field data for aquatic macrophyte studies

NASA Astrophysics Data System (ADS)

John, C. M.; Kavya, N.

2014-11-01

Aquatic macrophytes (AM) can serve as useful indicators of water pollution along the littoral zones. The spectral signatures of various AM were investigated to determine whether species could be discriminated by remote sensing. In this study the spectral readings of different AM communities identified were done using the ASD Fieldspec® Hand Held spectro-radiometer in the wavelength range of 325-1075 nm. The collected specific reflectance spectra were applied to space borne multi-spectral remote sensing data from Worldview-2, acquired on 26th March 2011. The dimensionality reduction of the spectro-radiometric data was done using the technique principal components analysis (PCA). Out of the different PCA axes generated, 93.472 % variance of the spectra was explained by the first axis. The spectral derivative analysis was done to identify the wavelength where the greatest difference in reflectance is shown. The identified wavelengths are 510, 690, 720, 756, 806, 885, 907 and 923 nm. The output of PCA and derivative analysis were applied to Worldview-2 satellite data for spectral subsetting. The unsupervised classification was used to effectively classify the AM species using the different spectral subsets. The accuracy assessment of the results of the unsupervised classification and their comparison were done. The overall accuracy of the result of unsupervised classification using the band combinations Red-Edge, Green, Coastal blue & Red-edge, Yellow, Blue is 100%. The band combinations NIR-1, Green, Coastal blue & NIR-1, Yellow, Blue yielded an accuracy of 82.35 %. The existing vegetation indices and new hyper-spectral indices for the different type of AM communities were computed. Overall, results of this study suggest that high spectral and spatial resolution images provide useful information for natural resource managers especially with regard to the location identification and distribution mapping of macrophyte species and their communities.

MEMS FPI-based smartphone hyperspectral imager

NASA Astrophysics Data System (ADS)

Rissanen, Anna; Saari, Heikki; Rainio, Kari; Stuns, Ingmar; Viherkanto, Kai; Holmlund, Christer; Näkki, Ismo; Ojanen, Harri

2016-05-01

This paper demonstrates a mobile phone- compatible hyperspectral imager based on a tunable MEMS Fabry-Perot interferometer. The realized iPhone 5s hyperspectral imager (HSI) demonstrator utilizes MEMS FPI tunable filter for visible-range, which consist of atomic layer deposited (ALD) Al2O3/TiO2-thin film Bragg reflectors. Characterization results for the mobile phone hyperspectral imager utilizing MEMS FPI chip optimized for 500 nm is presented; the operation range is λ = 450 - 550 nm with FWHM between 8 - 15 nm. Also a configuration of two cascaded FPIs (λ = 500 nm and λ = 650 nm) combined with an RGB colour camera is presented. With this tandem configuration, the overall wavelength tuning range of MEMS hyperspectral imagers can be extended to cover a larger range than with a single FPI chip. The potential applications of mobile hyperspectral imagers in the vis-NIR range include authentication, counterfeit detection and potential health/wellness and food sensing applications.

Developing a NIR multispectral imaging for prediction and visualization of peanut protein content using variable selection algorithms

NASA Astrophysics Data System (ADS)

Cheng, Jun-Hu; Jin, Huali; Liu, Zhiwei

2018-01-01

The feasibility of developing a multispectral imaging method using important wavelengths from hyperspectral images selected by genetic algorithm (GA), successive projection algorithm (SPA) and regression coefficient (RC) methods for modeling and predicting protein content in peanut kernel was investigated for the first time. Partial least squares regression (PLSR) calibration model was established between the spectral data from the selected optimal wavelengths and the reference measured protein content ranged from 23.46% to 28.43%. The RC-PLSR model established using eight key wavelengths (1153, 1567, 1972, 2143, 2288, 2339, 2389 and 2446 nm) showed the best predictive results with the coefficient of determination of prediction (R2P) of 0.901, and root mean square error of prediction (RMSEP) of 0.108 and residual predictive deviation (RPD) of 2.32. Based on the obtained best model and image processing algorithms, the distribution maps of protein content were generated. The overall results of this study indicated that developing a rapid and online multispectral imaging system using the feature wavelengths and PLSR analysis is potential and feasible for determination of the protein content in peanut kernels.

Growth, structural, optical and thermal properties of gamma-glycine crystal.

PubMed

Balakrishnan, T; Babu, R Ramesh; Ramamurthi, K

2008-04-01

Single crystals of gamma-glycine were grown from a mixture of glycine, water and lithium bromide. Single crystal X-ray diffraction analysis confirmed the growth of gamma-glycine phase. Presence of various functional groups of gamma-glycine was identified by FTIR spectrum. Optical absorbance spectrum recorded in the wavelength range of UV-vis-NIR revealed that this crystal has good optical transparency in the range 250-1500 nm. Vickers microhardness values were estimated on the prominent (100) face. Thermogravimetric and differential scanning calorimetric analyses were carried out to study the thermal properties of gamma-glycine. Second harmonic generation efficiency of the crystal measured by Kurtz's powder method using Nd:YAG laser is about three times that of KDP.

[Distribution Characteristics of Nitrifiers and Denitrifiers in the River Sediments of Tongling City].

PubMed

Cheng, Jian-hua; Dou, Zhi-yong; Sun, Qing-ye

2016-04-15

Rivers in mining areas were influenced by contaminants such as nitrogen, phosphorus and organic matter due to domestic and agricultural wastewater discharge in addition to pollutants caused by mining activities. In this study, surface sediment samples of rivers in Tongling city were collected to address the effect of season and pollution type on the abundance of nitrifiers and denitrifiers using quantitative polymerase chain reaction (QPCR) technique targeting at the ammonia monooxygenase (amoA) and nitrite reductase (nir) genes. The results showed that the average ahundance of ammonia oxidizing archaea (AGA) (ranging from 1.74 x 10⁵ to 1.45 x 10⁸ copies · g⁻¹) was 4.39 times that of ammonia oxidizing hacteria (AGH) (ranging from 1.39 x 10⁵ to 3.39 x 10⁷ copies · g⁻¹); and the average abundance of nirK gene (ranging from 4.45 x 10⁶ to 1.51 x 10⁸ copies · g) was almost a thirtieth part of nirS gene (ranging from 1.69 x 10⁷ to 8.55 x 10⁹ copies · g⁻¹). The abundance of AOA was higher in spring and autumn, and lower in summer and winter. And sediment AOB abundance was higher in spring and winter than in summer and autumn. Meanwhile, the abundance of nir genes was in the order of spring (nirS )/autumn (nirK) > summer > winter > autumn (nirS )/spring (nirK). Moreover, the abundance of bacterial and archaeal arnoA and nirS genes in sediments influenced by mine pollution was generally higher than that in sediments influenced by agricultural non-point pollution, whereas the abundance of nirK gene showed an opposite trend.

Plasmonic Nanowires for Wide Wavelength Range Molecular Sensing.

PubMed

Marinaro, Giovanni; Das, Gobind; Giugni, Andrea; Allione, Marco; Torre, Bruno; Candeloro, Patrizio; Kosel, Jurgen; Di Fabrizio, Enzo

2018-05-17

In this paper, we propose the use of a standing nanowires array, constituted by plasmonic active gold wires grown on iron disks, and partially immersed in a supporting alumina matrix, for surface-enhanced Raman spectroscopy applications. The galvanic process was used to fabricate nanowires in pores of anodized alumina template, making this device cost-effective. This fabrication method allows for the selection of size, diameter, and spatial arrangement of nanowires. The proposed device, thanks to a detailed design analysis, demonstrates a broadband plasmonic enhancement effect useful for many standard excitation wavelengths in the visible and NIR. The trigonal pores arrangement gives an efficiency weakly dependent on polarization. The devices, tested with 633 and 830 nm laser lines, show a significant Raman enhancement factor, up to around 6 × 10⁴, with respect to the flat gold surface, used as a reference for the measurements of the investigated molecules.

Fiber Delivery of mid-IR lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kriesel, J.M.; Gat, N.; Bernacki, Bruce E.

2011-08-24

Fiber optics for the visible to near infrared (NIR) wavelength regimes (i.e. = 0.42 {mu}m) have proven to be extremely useful for a myriad of applications such as telecommunications, illumination, and sensors because they enable convenient, compact, and remote delivery of laser beams. Similarly, there is a need for fiber optics operating at longer wavelengths. For example, systems operating in the mid-IR regime (i.e., = 314 {mu}m) are being developed to detect trace molecular species with far-reaching applications, such as detecting explosives on surfaces, pollutants in the environment, and biomarkers in the breath of a patient. Furthermore, with the increasingmore » availability of quantum cascade lasers (QCLs) which are semiconductor lasers that operate in the mid-IR regime additional uses are rapidly being developed. Here, we describe the development of hollow-core fibers for delivery of high-quality mid-IR laser beams across a broad spectral range.« less

Prediction of canned black bean texture (Phaseolus vulgaris L.) from intact dry seeds using visible/near infrared spectroscopy and hyperspectral imaging data.

PubMed

Mendoza, Fernando A; Cichy, Karen A; Sprague, Christy; Goffnett, Amanda; Lu, Renfu; Kelly, James D

2018-01-01

Texture is a major quality parameter for the acceptability of canned whole beans. Prior knowledge of this quality trait before processing would be useful to guide variety development by bean breeders and optimize handling protocols by processors. The objective of this study was to evaluate and compare the predictive power of visible and near infrared reflectance spectroscopy (visible/NIRS, 400-2498 nm) and hyperspectral imaging (HYPERS, 400-1000 nm) techniques for predicting texture of canned black beans from intact dry seeds. Black beans were grown in Michigan (USA) over three field seasons. The samples exhibited phenotypic variability for canned bean texture due to genetic variability and processing practice. Spectral preprocessing methods (i.e. smoothing, first and second derivatives, continuous wavelet transform, and two-band ratios), coupled with a feature selection method, were tested for optimizing the prediction accuracy in both techniques based on partial least squares regression (PLSR) models. Visible/NIRS and HYPERS were effective in predicting texture of canned beans using intact dry seeds, as indicated by their correlation coefficients for prediction (R pred ) and standard errors of prediction (SEP). Visible/NIRS was superior (R pred = 0.546-0.923, SEP = 7.5-1.9 kg 100 g -1 ) to HYPERS (R pred = 0.401-0.883, SEP = 7.6-2.4 kg 100 g -1 ), which is likely due to the wider wavelength range collected in visible/NIRS. However, a significant improvement was reached in both techniques when the two-band ratios preprocessing method was applied to the data, reducing SEP by at least 10.4% and 16.2% for visible/NIRS and HYPERS, respectively. Moreover, results from using the combination of the three-season data sets based on the two-band ratios showed that visible/NIRS (R pred = 0.886, SEP = 4.0 kg 100 g -1 ) and HYPERS (R pred = 0.844, SEP = 4.6 kg 100 g -1 ) models were consistently successful in predicting texture over a wide range of measurements. Visible/NIRS and HYPERS have great potential for predicting the texture of canned beans; the robustness of the models is impacted by genotypic diversity, planting year and phenotypic variability for canned bean texture used for model building, and hence, robust models can be built based on data sets with high phenotypic diversity in textural properties, and periodically maintained and updated with new data. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Fast detection and visualization of minced lamb meat adulteration using NIR hyperspectral imaging and multivariate image analysis.

PubMed

Kamruzzaman, Mohammed; Sun, Da-Wen; ElMasry, Gamal; Allen, Paul

2013-01-15

Many studies have been carried out in developing non-destructive technologies for predicting meat adulteration, but there is still no endeavor for non-destructive detection and quantification of adulteration in minced lamb meat. The main goal of this study was to develop and optimize a rapid analytical technique based on near-infrared (NIR) hyperspectral imaging to detect the level of adulteration in minced lamb. Initial investigation was carried out using principal component analysis (PCA) to identify the most potential adulterate in minced lamb. Minced lamb meat samples were then adulterated with minced pork in the range 2-40% (w/w) at approximately 2% increments. Spectral data were used to develop a partial least squares regression (PLSR) model to predict the level of adulteration in minced lamb. Good prediction model was obtained using the whole spectral range (910-1700 nm) with a coefficient of determination (R(2)(cv)) of 0.99 and root-mean-square errors estimated by cross validation (RMSECV) of 1.37%. Four important wavelengths (940, 1067, 1144 and 1217 nm) were selected using weighted regression coefficients (Bw) and a multiple linear regression (MLR) model was then established using these important wavelengths to predict adulteration. The MLR model resulted in a coefficient of determination (R(2)(cv)) of 0.98 and RMSECV of 1.45%. The developed MLR model was then applied to each pixel in the image to obtain prediction maps to visualize the distribution of adulteration of the tested samples. The results demonstrated that the laborious and time-consuming tradition analytical techniques could be replaced by spectral data in order to provide rapid, low cost and non-destructive testing technique for adulterate detection in minced lamb meat. Copyright © 2012 Elsevier B.V. All rights reserved.

Laboratory spectroscopy of meteorite samples at UV-vis-NIR wavelengths: Analysis and discrimination by principal components analysis

NASA Astrophysics Data System (ADS)

Penttilä, Antti; Martikainen, Julia; Gritsevich, Maria; Muinonen, Karri

2018-02-01

Meteorite samples are measured with the University of Helsinki integrating-sphere UV-vis-NIR spectrometer. The resulting spectra of 30 meteorites are compared with selected spectra from the NASA Planetary Data System meteorite spectra database. The spectral measurements are transformed with the principal component analysis, and it is shown that different meteorite types can be distinguished from the transformed data. The motivation is to improve the link between asteroid spectral observations and meteorite spectral measurements.

Tracheid length prediction in Pinus palustris by means of near infrared spectroscopy: the influence of age

Treesearch

Brian K. Via; Todd F. Shupe; Michael Stine; Chi-Leung So; Leslie H. Groom

2005-01-01

The predidion of tracheid Length using near infrared (NIR) wavelengths can provide either useful or misleading calibrations depending on the context. This can happen since tracheid length is not directly related to the absorbance at any wavelength but is instead the result of a seondary correlation with some unknown chemical constituent. In this work, the effect of...

Analysis of silage composition by near-infrared reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Reeves, James B., III; Blosser, Timothy H.; Colenbrander, V. F.

1991-02-01

Two studies were performed to investigate the feasibility of using near infrared reflectance spectroscopy (NIRS) with undried silages. In the first study silages were analyzed for major components (e. g. dry matter crude protein and other forms of nitrogen fiber and in vitro digestible dry matter) and short chain fatty acids (SCFA). NIRS was found to operate satisfactorily except for some forms of nitrogen and SCFA. In study two various methods of grinding spectral regions and sample presentation were examined. Undried Wiley ground samples in a rectangular cell gave the best overall results for non-dry ice undried grinds with wavelengths between 1100 and 2498 nm. Silages scanned after drying however produced the best results. Intact samples did not perform as well as ground samples and wavelengths below 1100 nm were of little use. 2 .

Deep brain two-photon NIR fluorescence imaging for study of Alzheimer's disease

NASA Astrophysics Data System (ADS)

Chen, Congping; Liang, Zhuoyi; Zhou, Biao; Ip, Nancy Y.; Qu, Jianan Y.

2018-02-01

Amyloid depositions in the brain represent the characteristic hallmarks of Alzheimer's disease (AD) pathology. The abnormal accumulation of extracellular amyloid-beta (Aβ) and resulting toxic amyloid plaques are considered to be responsible for the clinical deficits including cognitive decline and memory loss. In vivo two-photon fluorescence imaging of amyloid plaques in live AD mouse model through a chronic imaging window (thinned skull or craniotomy) provides a mean to greatly facilitate the study of the pathological mechanism of AD owing to its high spatial resolution and long-term continuous monitoring. However, the imaging depth for amyloid plaques is largely limited to upper cortical layers due to the short-wavelength fluorescence emission of commonly used amyloid probes. In this work, we reported that CRANAD-3, a near-infrared (NIR) probe for amyloid species with excitation wavelength at 900 nm and emission wavelength around 650 nm, has great advantages over conventionally used probes and is well suited for twophoton deep imaging of amyloid plaques in AD mouse brain. Compared with a commonly used MeO-X04 probe, the imaging depth of CRANAD-3 is largely extended for open skull cranial window. Furthermore, by using two-photon excited fluorescence spectroscopic imaging, we characterized the intrinsic fluorescence of the "aging pigment" lipofuscin in vivo, which has distinct spectra from CRANAD-3 labeled plaques. This study reveals the unique potential of NIR probes for in vivo, high-resolution and deep imaging of brain amyloid in Alzheimer's disease.

Contrast-enhanced photoacoustic imaging with an optical wavelength of 1064 nm

NASA Astrophysics Data System (ADS)

Kim, Jeesu; Park, Sara; Park, Gyeong Bae; Choi, Wonseok; Jeong, Unyong; Kim, Chulhong

2018-02-01

Photoacoustic (PA) imaging is a biomedical imaging method that can provide both structural and functional information of living tissues beyond the optical diffusion limit by combining the concepts of conventional optical and ultrasound imaging methods. Although endogenous chromophores can be utilized to acquire PA images of biological tissues, exogenous contrast agents that absorb near-infrared (NIR) lights have been extensively explored to improve the contrast and penetration depth of PA images. Here, we demonstrate Bi2Se3 nanoplates, that strongly absorbs NIR lights, as a contrast agent for PA imaging. In particularly, the Bi2Se3 nanoplates produce relatively strong PA signals with an optical wavelength of 1064 nm, which has several advantages for deep tissue imaging including: (1) relatively low absorption by other intrinsic chromophores, (2) cost-effective light source using Nd:YAG laser, and (3) higher available energy than other NIR lights according to American National Standards Institute (ANSI) safety limit. We have investigated deep tissue imaging capability of the Bi2Se3 nanoplates by acquiring in vitro PA images of microtubes under chicken breast tissues. We have also acquired in vivo PA images of bladders, gastrointestinal tracts, and sentinel lymph nodes in mice after injection of the Bi2Se3 nanoplates to verify their applicability to a variety of biomedical research. The results show the promising potential of the Bi2Se3 nanoplates as a PA contrast agent for deep tissue imaging with an optical wavelength of 1064 nm.

Second and third NIR optical windows for imaging of bone microfractures

NASA Astrophysics Data System (ADS)

Sordillo, Laura A.; Pu, Yang; Sordillo, Peter P.; Budansky, Yury; Alfano, R. R.

2014-05-01

Microfractures in bone, secondary to repetitive stress, particularly in the lower extremities, are an important problem for military recruits and for athletes. They also may occur in those with brittle bones, such as the elderly, or in patients taking bisphosphonates for osteoporosis. Microfractures can be early predictors of major bone fracture and may be as important as changes in bone density in predicting where and how likely a major fracture will occur. Unlike major bone fractures, microfractures can be difficult to detect by conventional methods. We explored a second NIR spectral window from 1,100 nm to 1,350 nm, and a third spectral window from 1,600 nm to 1,870 nm to image microfractures through tissue media. Due to a reduction in scattering at longer NIR wavelengths, employment of the second and third NIR windows may allow for deeper penetration into tissue and higher contrast images of microfractures underneath the skin.

Assessment of simulated lesions on primary teeth with near-infrared imaging

NASA Astrophysics Data System (ADS)

Tam, Wilson; Lee, Robert C.; Lin, Brent; Simon, Jacob C.; Fried, Daniel

2016-02-01

Previous studies have demonstrated that the structural changes on enamel due to demineralization and remineralization can be exploited through optical imaging methods such as QLF, thermal and NIR imaging. The purpose of this study is to investigate whether PS-OCT and NIR reflectance imaging can be utilized to assess lesion structure in artificial enamel lesions on the smooth surfaces of primary teeth exposed to fluoride. The smooth coronal surfaces of primary teeth (n=25) were divided into 4 windows: sound, demineralization, demineralization with remineralization and APF with demineralization. Windows were treated with either acidulated phosphate fluoride (APF) for 1 minute, a demineralization solution for 4 days, and/or an acidic remineralization solution for 12 days. The samples were imaged using PS-OCT, QLF and NIR reflectance at 1400-1700 nm wavelengths. This study demonstrated that both PS-OCT and NIR reflectance imaging were suitable for assessing lesion structure in the smooth surfaces of primary dentition.

Assessment of simulated lesions on primary teeth with near-infrared imaging.

PubMed

Tam, Wilson; Lee, Robert C; Lin, Brent; Simon, Jacob C; Fried, Daniel

2016-02-13

Previous studies have demonstrated that the structural changes on enamel due to demineralization and remineralization can be exploited through optical imaging methods such as QLF, thermal and NIR imaging. The purpose of this study is to investigate whether PS-OCT and NIR reflectance imaging can be utilized to assess lesion structure in artificial enamel lesions on the smooth surfaces of primary teeth exposed to fluoride. The smooth coronal surfaces of primary teeth (n=25) were divided into 4 windows: sound, demineralization, demineralization with remineralization and APF with demineralization. Windows were treated with either acidulated phosphate fluoride (APF) for 1 minute, a demineralization solution for 4 days, and/or an acidic remineralization solution for 12 days. The samples were imaged using PS-OCT, QLF and NIR reflectance at 1400-1700 nm wavelengths. This study demonstrated that both PS-OCT and NIR reflectance imaging were suitable for assessing lesion structure in the smooth surfaces of primary dentition.

High-fidelity entanglement between a trapped ion and a telecom photon via quantum frequency conversion.

PubMed

Bock, Matthias; Eich, Pascal; Kucera, Stephan; Kreis, Matthias; Lenhard, Andreas; Becher, Christoph; Eschner, Jürgen

2018-05-21

Entanglement between a stationary quantum system and a flying qubit is an essential ingredient of a quantum-repeater network. It has been demonstrated for trapped ions, trapped atoms, color centers in diamond, or quantum dots. These systems have transition wavelengths in the blue, red or near-infrared spectral regions, whereas long-range fiber-communication requires wavelengths in the low-loss, low-dispersion telecom regime. A proven tool to interconnect flying qubits at visible/NIR wavelengths to the telecom bands is quantum frequency conversion. Here we use an efficient polarization-preserving frequency converter connecting 854 nm to the telecom O-band at 1310 nm to demonstrate entanglement between a trapped 40 Ca + ion and the polarization state of a telecom photon with a high fidelity of 98.2 ± 0.2%. The unique combination of 99.75 ± 0.18% process fidelity in the polarization-state conversion, 26.5% external frequency conversion efficiency and only 11.4 photons/s conversion-induced unconditional background makes the converter a powerful ion-telecom quantum interface.

Optimum efficiency lidar sensing of multilayer hydrometeors through a turbid atmosphere

NASA Astrophysics Data System (ADS)

Evgenieva, Ts T.; Gurdev, L. L.

2018-03-01

The detected lidar return power is a basic factor determining the brightness of the detected lidar images and the signal-to-noise ratio (SNR) of a given measurement. At equal other characteristics, the laser radiation wavelength should influence the lidar return signal and assume an optimum value depending on the specificity of the objects investigated. As such a problem had not been considered systematically, we recently began developing a modeling approach to solving it, based on evaluating the mean and the noisy lidar profiles and the SNR profile of the measurement along the lidar line of sight by using the lidar equation and well known realistic models of the atmospheric objects and background. The main purpose of the present work is to estimate by numerical modeling the detectability of the lidar return from different distances and multilayer cirrus clouds, depending on the laser radiation wavelengths. The results obtained confirm the expectations that at a higher atmospheric turbidity, a relatively higher sensing efficiency (return power) is achievable by longer-wavelength laser radiation, within the NIR range.

CR-39 (PADC) Reflection and Transmission of Light in the Ultraviolet-Near-Infrared (UV-NIR) Range.

PubMed

Traynor, Nathan B J; McLauchlin, Christopher; Dodge, Kenneth; McGarrah, James E; Padalino, Stephen J; McCluskey, Michelle; Sangster, T C; McLean, James G

2018-04-01

The spectral reflection (specular and diffuse) and transmission of Columbia Resin 39 (CR-39) were measured for incoherent light with wavelengths in the range of 200-2500 nm. These results will be of use for the optical characterization of CR-39, as well as in investigations of the chemical modifications of the polymer caused by ultraviolet (UV) exposure. A Varian Cary 5000 was used to perform spectroscopy on several different thicknesses of CR-39. With proper analysis for the interdependence of reflectance and transmittance, results are consistent across all samples. The reflectivity from each CR-39-air boundary reveals an increase in the index of refraction in the near-UV. Absorption observations are consistent with the Beer-Lambert law. Strong absorption of UV light of wavelength shorter than 350 nm suggests an optical band gap of 3.5 eV, although the standard analysis is not conclusive. Absorption features observed in the near infrared are assigned to molecular vibrations, including some that are new to the literature.

Early optical detection of cerebral edema in vivo.

PubMed

Gill, Amandip S; Rajneesh, Kiran F; Owen, Christopher M; Yeh, James; Hsu, Mike; Binder, Devin K

2011-02-01

Cerebral edema is a significant cause of morbidity and mortality in diverse disease states. Currently, the means to detect progressive cerebral edema in vivo includes the use of intracranial pressure (ICP) monitors and/or serial radiological studies. However, ICP measurements exhibit a high degree of variability, and ICP monitors detect edema only after it becomes sufficient to significantly raise ICP. The authors report the development of 2 distinct minimally invasive fiberoptic near-infrared (NIR) techniques able to directly detect early cerebral edema. Cytotoxic brain edema was induced in adult CD1 mice via water intoxication by intraperitoneal water administration (30% body weight intraperitoneally). An implantable dual-fiberoptic probe was stereotactically placed into the cerebral cortex and connected to optical source and detector hardware. Optical sources consisted of either broadband halogen illumination or a single-wavelength NIR laser diode, and the detector was a sensitive NIR spectrometer or optical power meter. In one subset of animals, a left-sided craniectomy was performed to obtain cortical biopsies for water-content determination to verify cerebral edema. In another subset of animals, an ICP transducer was placed on the contralateral cortex, which was synchronized to a computer and time stamped. Using either broadband illumination with NIR spectroscopy or single-wavelength laser diode illumination with optical power meter detection, the authors detected a reduction in NIR optical reflectance during early cerebral edema. The time intervals between water injection (Time Point 0), optical trigger (defined as a 2-SD change in optical reflectance from baseline), and defined threshold ICP values of 10, 15 and 20 mm Hg were calculated. Reduction in NIR reflectance occurred significantly earlier than any of the ICP thresholds (p < 0.001). Saline-injected control mice exhibited a steady baseline optical signal. There was a significant correlation between reflectance change and tissue specific gravity of the cortical biopsies, further validating the dual-fiberoptic probe as a direct measure of cerebral edema. Compared with traditional ICP monitoring, the aforementioned minimally invasive NIR techniques allow for the significantly earlier detection of cerebral edema, which may be of clinical utility in the identification and thus early treatment of cerebral edema.

Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

NASA Astrophysics Data System (ADS)

Neves, Luís F. F.; Krais, John J.; Van Rite, Brent D.; Ramesh, Rajagopal; Resasco, Daniel E.; Harrison, Roger G.

2013-09-01

This paper focuses on the targeting of single-walled carbon nanotubes (SWNTs) for the treatment of breast cancer with minimal side effects using photothermal therapy. The human protein annexin V (AV) binds specifically to anionic phospholipids expressed externally on the surface of tumour cells and endothelial cells that line the tumour vasculature. A 2 h incubation of the SWNT-AV conjugate with proliferating endothelial cells followed by washing and near-infrared (NIR) irradiation at a wavelength of 980 nm was enough to induce significant cell death; there was no significant cell death with irradiation or the conjugate alone. Administration of the same conjugate i.v. in BALB/c female mice with implanted 4T1 murine mammary at a dose of 0.8 mg SWNT kg-1 and followed one day later by NIR irradiation of the tumour at a wavelength of 980 nm led to complete disappearance of implanted 4T1 mouse mammary tumours for the majority of the animals by 11 days since the irradiation. The combination of the photothermal therapy with the immunoadjuvant cyclophosphamide resulted in increased survival. The in vivo results suggest the SWNT-AV/NIR treatment is a promising approach to treat breast cancer.

System-level analysis and design for RGB-NIR CMOS camera

NASA Astrophysics Data System (ADS)

Geelen, Bert; Spooren, Nick; Tack, Klaas; Lambrechts, Andy; Jayapala, Murali

2017-02-01

This paper presents system-level analysis of a sensor capable of simultaneously acquiring both standard absorption based RGB color channels (400-700nm, 75nm FWHM), as well as an additional NIR channel (central wavelength: 808 nm, FWHM: 30nm collimated light). Parallel acquisition of RGB and NIR info on the same CMOS image sensor is enabled by monolithic pixel-level integration of both a NIR pass thin film filter and NIR blocking filters for the RGB channels. This overcomes the need for a standard camera-level NIR blocking filter to remove the NIR leakage present in standard RGB absorption filters from 700-1000nm. Such a camera-level NIR blocking filter would inhibit the acquisition of the NIR channel on the same sensor. Thin film filters do not operate in isolation. Rather, their performance is influenced by the system context in which they operate. The spectral distribution of light arriving at the photo diode is shaped a.o. by the illumination spectral profile, optical component transmission characteristics and sensor quantum efficiency. For example, knowledge of a low quantum efficiency (QE) of the CMOS image sensor above 800nm may reduce the filter's blocking requirements and simplify the filter structure. Similarly, knowledge of the incoming light angularity as set by the objective lens' F/# and exit pupil location may be taken into account during the thin film's optimization. This paper demonstrates how knowledge of the application context can facilitate filter design and relax design trade-offs and presents experimental results.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Zhe, E-mail: zhenzhe1201@sina.com; Yang, Lei; Hang, Yin

Highly transparent Dy{sup 3+}-doped terbium gallium garnet (TGG) single crystal was grown by Czochralski (Cz) method. Phase composition of the crystal was tested by XRD measurements. The distribution coefficient of Dy{sup 3+} in the crystal was obtained. The optical and magneto-optical properties were analyzed in detail, and magnetic properties of the Dy{sup 3+}-TGG crystal were studied. The paramagnetic behavior is observed down to 10 K. The as-grown crystal exhibited high optical transmittance, particularly in the visible region. The Faraday rotation was investigated over visible and near-infrared regions (VIS–NIR) at room temperature. The Verdet constants increase at measured wavelengths and highmore » thermal stability was found in Dy{sup 3+}-doped TGG, as compared to the properties of pure TGG, indicating that Dy{sup 3+}-doped crystals are preferable for magneto-active materials used in Faraday devices at VIS–NIR wavelengths. - Graphical abstract: Highly transparent Dy{sup 3+}-doped terbium gallium garnet (TGG) and pure TGG single crystals were grown by Czochralski method. The Dy{sup 3+}-doped TGG possesses 20–30% higher Verdet values in reference to TGG independently on wavelength.« less

Laboratory and Field Spectroscopy of Moon analogue material

NASA Astrophysics Data System (ADS)

Offringa, Marloes; Foing, Bernard H.

2016-07-01

Samples derived from terrestrial analogue sites are studied to gain insight into lunar processes in their geological context (Foing, Stoker, Ehrenfreund, 2011). For this study samples from the volcanic region of the Eifel, Germany collected during our latest field campaigns in November 2015 and February 2016 (Foing et al., 2010), are analyzed with a variety of spectrometers. The aim is to obtain a database of analyzed samples that could be used as a reference for future in situ measurements. We also use a documented set of Moon-Mars relevant minerals curated at VU Amsterdam. We are using systematically for all samples UV-VIS and NIR reflectance spectrometers, and sporadically a Fourier Transform Infrared (FTIR) spectrometer, an X-Ray Fluorescence (XRF) spectrometer and a Raman laser spectrometer on control samples. Calibration of the UV-VIS and NIR reflectance spectrometers is the main focus of this research in order to obtain the clearest spectra. The calibration of the UV-VIS and NIR reflectance spectrometers requires the use of a good light source as well as suitable optical fibers to create a signal that covers the widest range in wavelengths available. To eliminate noise towards the edges of this range, multiple measurements are averaged and data is processed by dividing the signal by reference spectra. Obtained spectra can be tested for accuracy by comparing them with stationary laboratory spectrometers such as the FTIR spectrometer. The Raman, UV-VIS and NIR are also used in combination with the ExoGeoLab mock-up lander during field campaigns (Foing, Stoker, Ehrenfreund, 2011) also brought again to Eifel in February 2016, to prove the applicability of the equipment in the field. Acknowledgements: we thank Dominic Doyle for ESTEC optical lab support, Euan Monaghan (Leiden U) for FTIR measurement support, Wim van Westrenen for access to VU samples, Oscar Kamps (Utrecht U./ESTEC), Aidan Cowley (EAC) and Matthias Sperl (DLR) for support discussions

Strategies for multivariate modeling of moisture content in freeze-dried mannitol-containing products by near-infrared spectroscopy.

PubMed

Yip, Wai Lam; Gausemel, Ingvil; Sande, Sverre Arne; Dyrstad, Knut

2012-11-01

Accurate determination of residual moisture content of a freeze-dried (FD) pharmaceutical product is critical for prediction of its quality. Near-infrared (NIR) spectroscopy is a fast and non-invasive method routinely used for quantification of moisture. However, several physicochemical properties of the FD product may interfere with absorption bands related to the water content. A commonly used stabilizer and bulking agent in FD known for variation in physicochemical properties, is mannitol. To minimize this physicochemical interference, different approaches for multivariate correlation between NIR spectra of a FD product containing mannitol and the corresponding moisture content measured by Karl Fischer (KF) titration have been investigated. A novel method, MIPCR (Main and Interactions of Individual Principal Components Regression), was found to have significantly increased predictive ability of moisture content compared to a traditional PLS approach. The philosophy behind the MIPCR is that the interference from a variety of particle and morphology attributes has interactive effects on the water related absorption bands. The transformation of original wavelength variables to orthogonal scores gives a new set of variables (scores) without covariance structure, and the possibility of inclusion of interaction terms in the further modeling. The residual moisture content of the FD product investigated is in the range from 0.7% to 2.6%. The mean errors of cross validated prediction of models developed in the investigated NIR regions were reduced from a range of 24.1-27.6% for traditional PLS method to 15.7-20.5% for the MIPCR method. Improved model quality by application of MIPCR, without the need for inclusion of a large number of calibration samples, might increase the use of NIR in early phase product development, where availability of calibration samples is often limited. Copyright © 2012 Elsevier B.V. All rights reserved.

Application of NIRS coupled with PLS regression as a rapid, non-destructive alternative method for quantification of KBA in Boswellia sacra

NASA Astrophysics Data System (ADS)

Al-Harrasi, Ahmed; Rehman, Najeeb Ur; Mabood, Fazal; Albroumi, Muhammaed; Ali, Liaqat; Hussain, Javid; Hussain, Hidayat; Csuk, René; Khan, Abdul Latif; Alam, Tanveer; Alameri, Saif

2017-09-01

In the present study, for the first time, NIR spectroscopy coupled with PLS regression as a rapid and alternative method was developed to quantify the amount of Keto-β-Boswellic Acid (KBA) in different plant parts of Boswellia sacra and the resin exudates of the trunk. NIR spectroscopy was used for the measurement of KBA standards and B. sacra samples in absorption mode in the wavelength range from 700-2500 nm. PLS regression model was built from the obtained spectral data using 70% of KBA standards (training set) in the range from 0.1 ppm to 100 ppm. The PLS regression model obtained was having R-square value of 98% with 0.99 corelationship value and having good prediction with RMSEP value 3.2 and correlation of 0.99. It was then used to quantify the amount of KBA in the samples of B. sacra. The results indicated that the MeOH extract of resin has the highest concentration of KBA (0.6%) followed by essential oil (0.1%). However, no KBA was found in the aqueous extract. The MeOH extract of the resin was subjected to column chromatography to get various sub-fractions at different polarity of organic solvents. The sub-fraction at 4% MeOH/CHCl3 (4.1% of KBA) was found to contain the highest percentage of KBA followed by another sub-fraction at 2% MeOH/CHCl3 (2.2% of KBA). The present results also indicated that KBA is only present in the gum-resin of the trunk and not in all parts of the plant. These results were further confirmed through HPLC analysis and therefore it is concluded that NIRS coupled with PLS regression is a rapid and alternate method for quantification of KBA in Boswellia sacra. It is non-destructive, rapid, sensitive and uses simple methods of sample preparation.

Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system

NASA Astrophysics Data System (ADS)

Venugopal, Vivek; Park, Minho; Ashitate, Yoshitomo; Neacsu, Florin; Kettenring, Frank; Frangioni, John V.; Gangadharan, Sidhu P.; Gioux, Sylvain

2013-12-01

We report the design, characterization, and validation of an optimized simultaneous color and near-infrared (NIR) fluorescence rigid endoscopic imaging system for minimally invasive surgery. This system is optimized for illumination and collection of NIR wavelengths allowing the simultaneous acquisition of both color and NIR fluorescence at frame rates higher than 6.8 fps with high sensitivity. The system employs a custom 10-mm diameter rigid endoscope optimized for NIR transmission. A dual-channel light source compatible with the constraints of an endoscope was built and includes a plasma source for white light illumination and NIR laser diodes for fluorescence excitation. A prism-based 2-CCD camera was customized for simultaneous color and NIR detection with a highly efficient filtration scheme for fluorescence imaging of both 700- and 800-nm emission dyes. The performance characterization studies indicate that the endoscope can efficiently detect fluorescence signal from both indocyanine green and methylene blue in dimethyl sulfoxide at the concentrations of 100 to 185 nM depending on the background optical properties. Finally, we performed the validation of this imaging system in vivo during a minimally invasive procedure for thoracic sentinel lymph node mapping in a porcine model.

Near-infrared light controlled photocatalytic activity of carbon quantum dots for highly selective oxidation reaction

NASA Astrophysics Data System (ADS)

Li, Haitao; Liu, Ruihua; Lian, Suoyuan; Liu, Yang; Huang, Hui; Kang, Zhenhui

2013-03-01

Selective oxidation of alcohols is a fundamental and significant transformation for the large-scale production of fine chemicals, UV and visible light driven photocatalytic systems for alcohol oxidation have been developed, however, the long wavelength near infrared (NIR) and infrared (IR) light have not yet fully utilized by the present photocatalytic systems. Herein, we reported carbon quantum dots (CQDs) can function as an effective near infrared (NIR) light driven photocatalyst for the selective oxidation of benzyl alcohol to benzaldehyde. Based on the NIR light driven photo-induced electron transfer property and its photocatalytic activity for H2O2 decomposition, this metal-free catalyst could realize the transformation from benzyl alcohol to benzaldehyde with high selectivity (100%) and conversion (92%) under NIR light irradiation. HO&z.rad; is the main active oxygen specie in benzyl alcohol selective oxidative reaction confirmed by terephthalic acid photoluminescence probing assay (TA-PL), selecting toluene as the substrate. Such metal-free photocatalytic system also selectively converts other alcohol substrates to their corresponding aldehydes with high conversion, demonstrating a potential application of accessing traditional alcohol oxidation chemistry.Selective oxidation of alcohols is a fundamental and significant transformation for the large-scale production of fine chemicals, UV and visible light driven photocatalytic systems for alcohol oxidation have been developed, however, the long wavelength near infrared (NIR) and infrared (IR) light have not yet fully utilized by the present photocatalytic systems. Herein, we reported carbon quantum dots (CQDs) can function as an effective near infrared (NIR) light driven photocatalyst for the selective oxidation of benzyl alcohol to benzaldehyde. Based on the NIR light driven photo-induced electron transfer property and its photocatalytic activity for H2O2 decomposition, this metal-free catalyst could realize the transformation from benzyl alcohol to benzaldehyde with high selectivity (100%) and conversion (92%) under NIR light irradiation. HO&z.rad; is the main active oxygen specie in benzyl alcohol selective oxidative reaction confirmed by terephthalic acid photoluminescence probing assay (TA-PL), selecting toluene as the substrate. Such metal-free photocatalytic system also selectively converts other alcohol substrates to their corresponding aldehydes with high conversion, demonstrating a potential application of accessing traditional alcohol oxidation chemistry. Electronic supplementary information (ESI) available. See DOI: 10.1039/c3nr00092c

Solar-Induced Plant Fluorescence as seen from space-borne instruments

NASA Astrophysics Data System (ADS)

Khosravi, Narges; Vountas, Marco; Rozanov, Vladimir V.; Bracher, Astrid; Burrows, John P.

2015-04-01

Solar induced chlorophyll fluorescence (SIF) retrieval can be linked to vegetation correspondence to global carbon cycle, and could be useful for terrestrial carbon budget assessment as well as agricultural and environmental purposes. There have been several investigations using space-borne SIF retrieval due to its good spatial coverage and time efficiency. These methods are mainly based on the fact that plant leaves absorb sunlight mainly within the visible spectral range and use it either for photosynthesis and/or release it as heat or fluorescence (in red and Near Infra Red, NIR, spectral region) back to the atmosphere. As a result, SIF can be considered an additive signal on top of the ground reflectance reaching TOA (Top Of the Atmosphere). Chlorophyll fluorescence is mainly emitted in the spectral range of red to the near-infrared with a pronounced peak at 690 and another at 740 nm. Although it is a very weak signal and two orders of magnitude smaller than the received radiance at TOA, it is feasible to retrieve it within spectral wavelength windows in the NIR. We developed a novel SIF retrieval method based on a modeled assumption of the emitted fluorescence spectrum at canopy level as it would be seen at TOA. The application of it to 10 years of SCIAMACHY (Scanning Imaging Absorption Spectrometer for Atmospheric CHartographY) data showed promising results. Comparing our SIF retrieval with results from other studies showed that SIF values of our retrieval are in a general agreement with them. With some variations. As there is no validated SIF retrieval, it is difficult to judge the retrieval quality. Our approach is of generic nature and therefore, could be applied to other data sets as well. Hence, the method is being applied on GOME-2 level 1 data, as the instrument has a better spatial resolution (in the wavelength range needed) and a better global coverage.

Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells.

PubMed

Wang, Yuguang; Huang, Ying-Ying; Wang, Yong; Lyu, Peijun; Hamblin, Michael R

2017-08-10

We previously showed that blue (415 nm) and green (540 nm) wavelengths were more effective in stimulating osteoblast differentiation of human adipose-derived stem cells (hASC), compared to red (660 nm) and near-infrared (NIR, 810 nm). Intracellular calcium was higher after blue/green, and could be inhibited by the ion channel blocker, capsazepine. In the present study we asked what was the effect of these four wavelengths on proliferation of the hASC? When cultured in proliferation medium there was a clear difference between blue/green which inhibited proliferation and red/NIR which stimulated proliferation, all at 3 J/cm 2 . Blue/green reduced cellular ATP, while red/NIR increased ATP in a biphasic manner. Blue/green produced a bigger increase in intracellular calcium and reactive oxygen species (ROS). Blue/green reduced mitochondrial membrane potential (MMP) and lowered intracellular pH, while red/NIR had the opposite effect. Transient receptor potential vanilloid 1 (TRPV1) ion channel was expressed in hADSC, and the TRPV1 ligand capsaicin (5uM) stimulated proliferation, which could be abrogated by capsazepine. The inhibition of proliferation caused by blue/green could also be abrogated by capsazepine, and by the antioxidant, N-acetylcysteine. The data suggest that blue/green light inhibits proliferation by activating TRPV1, and increasing calcium and ROS.

Development of an oxygen saturation measuring system by using near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Kono, K.; Nakamachi, E.; Morita, Y.

2017-08-01

Recently, the hypoxia imaging has been recognized as the advanced technique to detect cancers because of a strong relationship with the biological characterization of cancer. In previous studies, hypoxia imaging systems for endoscopic diagnosis have been developed. However, these imaging technologies using the visible light can observe only blood vessels in gastric mucous membrane. Therefore, they could not detect scirrhous gastric cancer which accounts for 10% of all gastric cancers and spreads rapidly into submucous membrane. To overcome this problem, we developed a measuring system of blood oxygen saturation in submucous membrane by using near-infrared (NIR) spectroscopy. NIR, which has high permeability for bio-tissues and high absorbency for hemoglobin, can image and observe blood vessels in submucous membrane. NIR system with LED lights and a CCD camera module was developed to image blood vessels. We measured blood oxygen saturation using the optical density ratio (ODR) of two wavelengths, based on Lambert-Beer law. To image blood vessel clearly and measure blood oxygen saturation accurately, we searched two optimum wavelengths by using a multilayer human gastric-like phantom which has same optical properties as human gastric one. By using Monte Carlo simulation of light propagation, we derived the relationship between the ODR and blood oxygen saturation and elucidated the influence of blood vessel depth on measuring blood oxygen saturation. The oxygen saturation measuring methodology was validated with experiments using our NIR system. Finally, it was confirmed that our system can detect oxygen saturation in various depth blood vessels accurately.

Solution-processable pyrite FeS(2) nanocrystals for the fabrication of heterojunction photodiodes with visible to NIR photodetection.

PubMed

Wang, Di-Yan; Jiang, You-Ting; Lin, Chih-Cheng; Li, Shao-Sian; Wang, Yaw-Tyng; Chen, Chia-Chun; Chen, Chun-Wei

2012-07-03

A heterojunction photodiode with NIR photoresponse using solution processable pyrite FeS(2) nanocrystal ink is demonstrated which has the advantages of earth-abundance and non-toxicity. The device consists of a FeS(2) nanocrystal (NC) thin film sandwiched with semiconducting metal oxides with a structure of ITO/ZnO/FeS(2) NC/MoO(3) /Au, which exhibits an excellent photoresponse with a spectral response extended to NIR wavelengths of up to 1150 nm and a high photocurrent/dark current ratio of up to 8000 at -1 V under AM1.5 illumination (100 mW cm(-2) ). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Near-infrared deep brain stimulation via upconversion nanoparticle–mediated optogenetics

NASA Astrophysics Data System (ADS)

Chen, Shuo; Weitemier, Adam Z.; Zeng, Xiao; He, Linmeng; Wang, Xiyu; Tao, Yanqiu; Huang, Arthur J. Y.; Hashimotodani, Yuki; Kano, Masanobu; Iwasaki, Hirohide; Parajuli, Laxmi Kumar; Okabe, Shigeo; Teh, Daniel B. Loong; All, Angelo H.; Tsutsui-Kimura, Iku; Tanaka, Kenji F.; Liu, Xiaogang; McHugh, Thomas J.

2018-02-01

Optogenetics has revolutionized the experimental interrogation of neural circuits and holds promise for the treatment of neurological disorders. It is limited, however, because visible light cannot penetrate deep inside brain tissue. Upconversion nanoparticles (UCNPs) absorb tissue-penetrating near-infrared (NIR) light and emit wavelength-specific visible light. Here, we demonstrate that molecularly tailored UCNPs can serve as optogenetic actuators of transcranial NIR light to stimulate deep brain neurons. Transcranial NIR UCNP-mediated optogenetics evoked dopamine release from genetically tagged neurons in the ventral tegmental area, induced brain oscillations through activation of inhibitory neurons in the medial septum, silenced seizure by inhibition of hippocampal excitatory cells, and triggered memory recall. UCNP technology will enable less-invasive optical neuronal activity manipulation with the potential for remote therapy.

[Characteristic wavelengths selection of soluble solids content of pear based on NIR spectral and LS-SVM].

PubMed

Fan, Shu-xiang; Huang, Wen-qian; Li, Jiang-bo; Zhao, Chun-jiang; Zhang, Bao-hua

2014-08-01

To improve the precision and robustness of the NIR model of the soluble solid content (SSC) on pear. The total number of 160 pears was for the calibration (n=120) and prediction (n=40). Different spectral pretreatment methods, including standard normal variate (SNV) and multiplicative scatter correction (MSC) were used before further analysis. A combination of genetic algorithm (GA) and successive projections algorithm (SPA) was proposed to select most effective wavelengths after uninformative variable elimination (UVE) from original spectra, SNV pretreated spectra and MSC pretreated spectra respectively. The selected variables were used as the inputs of least squares-support vector machine (LS-SVM) model to build models for de- termining the SSC of pear. The results indicated that LS-SVM model built using SNVE-UVE-GA-SPA on 30 characteristic wavelengths selected from full-spectrum which had 3112 wavelengths achieved the optimal performance. The correlation coefficient (Rp) and root mean square error of prediction (RMSEP) for prediction sets were 0.956, 0.271 for SSC. The model is reliable and the predicted result is effective. The method can meet the requirement of quick measuring SSC of pear and might be important for the development of portable instruments and online monitoring.

Near-infrared SN Ia Cosmology

NASA Astrophysics Data System (ADS)

Avelino, Arturo; Kirshner, Robert; Mandel, Kaisey; Challis, Peter; Friedman, Andrew; RAISIN Team

2018-01-01

Observations of SN Ia in the near infrared (NIR) are a promising way to construct an accurate cosmic expansion history to constrain the properties of dark energy. SN Ia are more nearly standard candles in NIR than in optical bands, while dust absorption is less of a problem at NIR wavelengths. This allows us to investigate the dark energy properties in a way that is less sensitive to systematic errors due to the variations in the intrinsic brightness of SN Ia or the properties of dust in their host galaxies. In this talk, I present preliminary results from our RAISIN 1 (HST GO-13046) and RAISIN 2 (HST GO-14216) programs with the Hubble Space Telescope, where we have constructed a Hubble diagram combining optical + NIR photometric data using a sample of low and high redshift SN Ia. I will discuss our current results, challenges, and the advantage of using optical + NIR data to derive accurate cosmic distances and improve knowledge of the dark energy equation of state. This research is supported by NSF grants AST-156854 and AST-1211196.

Design and performance of 4 x 5120-element visible and 2 x 2560-element shortwave infrared multispectral focal planes

NASA Astrophysics Data System (ADS)

Tower, J. R.; Cope, A. D.; Pellon, L. E.; McCarthy, B. M.; Strong, R. T.

1986-06-01

Two solid-state sensors for use in remote sensing instruments operating in the pushbroom mode are examined. The design and characteristics of the visible/near-infrared (VIS/NIR) device and the short-wavelength infrared (SWIR) device are described. The VIS/NIR is a CCD imager with four parallel sensor lines, each 1024 pixel long; the chip design and filter system of the VIS/NIR are studied. The performance of the VIS/NIR sensor with mask and its system performance are measured. The SWIR is a dual-band line imager consisting of palladium silicide Schottky-barrier detectors coupled to CCD multiplexers; the performance of the device is analyzed. The substrate materials and layout designs used to assemble the 4 x 5120-element VIS/NIR array and the 2 x 2560-element SWIR array are discussed, and the planarity of the butted arrays are verified using a profilometer. The optical and electrical characteristics, and the placement and butting accuracy of the arrays are evaluated. It is noted that the arrays met or exceed their expected performance.

Compressed single pixel imaging in the spatial frequency domain

PubMed Central

Torabzadeh, Mohammad; Park, Il-Yong; Bartels, Randy A.; Durkin, Anthony J.; Tromberg, Bruce J.

2017-01-01

Abstract. We have developed compressed sensing single pixel spatial frequency domain imaging (cs-SFDI) to characterize tissue optical properties over a wide field of view (35  mm×35  mm) using multiple near-infrared (NIR) wavelengths simultaneously. Our approach takes advantage of the relatively sparse spatial content required for mapping tissue optical properties at length scales comparable to the transport scattering length in tissue (ltr∼1  mm) and the high bandwidth available for spectral encoding using a single-element detector. cs-SFDI recovered absorption (μa) and reduced scattering (μs′) coefficients of a tissue phantom at three NIR wavelengths (660, 850, and 940 nm) within 7.6% and 4.3% of absolute values determined using camera-based SFDI, respectively. These results suggest that cs-SFDI can be developed as a multi- and hyperspectral imaging modality for quantitative, dynamic imaging of tissue optical and physiological properties. PMID:28300272

Prompt Emission of GRB 121217A from Gamma-Rays to the Near-Infrared

NASA Technical Reports Server (NTRS)

Elliott, J.; Yu, H.-F.; Schmidl, S.; Greiner, J.; Gruber, D.; Oates, S.; Kobayashi, S.; Zhang, B.; Cummings, J. R.; Filgas, R.;

Nondestructive sensing technologies using micro-optical elements for applications in the NIR-MIR spectral regions

NASA Astrophysics Data System (ADS)

Otto, Thomas; Saupe, Ray; Bruch, Reinhard F.; Fritzsch, Uwe; Stock, Volker; Gessner, Thomas; Afanasyeva, Natalia I.

2001-11-01

The field of microtechnology is an important industrial and scientific resource for the 21st century. There is a great interest in spectroscopic sensors in the near and middle infrared (NIR-MIR) wavelength regions (1 - 2.5 micrometers ; 2.5 - 4.5 micrometers ; 4 - 6 micrometers ). The potential for cheap and small devices for nondestructive, remote sensing techniques at a molecular level has stimulated the design and development of more compact analyzer systems. Therefore we will try to build analyzers using micro optical components such as micromirrors and embossed micro gratings optimized for the above mentioned spectral ranges. Potentially, infrared sensors can be used for rapid nondestructive diagnostics of surfaces, liquids, gases, polymers and complex biological systems including proteins, blood, cells and cellular debris as well as body tissue. Furthermore, NIR-MIR microsensing spectroscopy will be utilized to monitor the chemical composition of petrochemical products like gasoline and diesel. In addition, miniature analyzers will be used for rapid measuring of food, in particular oil, starch and meat. In this paper we will present an overview of several new approaches for subsurface and surface sensing technologies based on the integration of optical micro devices, the most promising sensors for biomedical, environmental and industrial applications, data processing and evaluation algorithms for classification of the results. Both scientific and industrial applications will be discussed.

Dynamic Features for Iris Recognition.

PubMed

da Costa, R M; Gonzaga, A

2012-08-01

The human eye is sensitive to visible light. Increasing illumination on the eye causes the pupil of the eye to contract, while decreasing illumination causes the pupil to dilate. Visible light causes specular reflections inside the iris ring. On the other hand, the human retina is less sensitive to near infra-red (NIR) radiation in the wavelength range from 800 nm to 1400 nm, but iris detail can still be imaged with NIR illumination. In order to measure the dynamic movement of the human pupil and iris while keeping the light-induced reflexes from affecting the quality of the digitalized image, this paper describes a device based on the consensual reflex. This biological phenomenon contracts and dilates the two pupils synchronously when illuminating one of the eyes by visible light. In this paper, we propose to capture images of the pupil of one eye using NIR illumination while illuminating the other eye using a visible-light pulse. This new approach extracts iris features called "dynamic features (DFs)." This innovative methodology proposes the extraction of information about the way the human eye reacts to light, and to use such information for biometric recognition purposes. The results demonstrate that these features are discriminating features, and, even using the Euclidean distance measure, an average accuracy of recognition of 99.1% was obtained. The proposed methodology has the potential to be "fraud-proof," because these DFs can only be extracted from living irises.

[Rapid prediction of annual ring density of Paulownia elongate standing tress using near infrared spectroscopy].

PubMed

Jiang, Ze-Hui; Wang, Yu-Rong; Fei, Ben-Hua; Fu, Feng; Hse, Chung-Yun

2007-06-01

Rapid prediction of annual ring density of Paulownia elongate standing trees using near infrared spectroscopy was studied. It was non-destructive to collect the samples for trees, that is, the wood cores 5 mm in diameter were unthreaded at the breast height of standing trees instead of fallen trees. Then the spectra data were collected by autoscan method of NIR. The annual ring density was determined by mercury immersion. And the models were made and analyzed by the partial least square (PLS) and full cross validation in the 350-2 500 nm wavelength range. The results showed that high coefficients were obtained between the annual ring and the NIR fitted data. The correlation coefficient of prediction model was 0.88 and 0.91 in the middle diameter and bigger diameter, respectively. Moreover, high coefficients of correlation were also obtained between annual ring density laboratory-determined and the NIR fitted data in the middle diameter of Paulownia elongate standing trees, the correlation coefficient of calibration model and prediction model were 0.90 and 0.83, and the standard errors of calibration (SEC) and standard errors of prediction(SEP) were 0.012 and 0.016, respectively. The method can simply, rapidly and non-destructively estimate the annual ring density of the Paulownia elongate standing trees close to the cutting age.

Four-wavelength near-infrared peripheral oximetry in cardiac surgery patients: a comparison between EQUANOX and O3.

PubMed

Ferraris, Arnaud; Jacquet-Lagrèze, Matthias; Fellahi, Jean-Luc

2018-04-01

Near-infrared spectroscopy (NIRS) is a continuous and noninvasive technology that measures regional tissue oxygen saturation (rSO 2 ). A new 4-wavelength generation of NIRS monitors is now available. We aimed to compare peripheral somatic rSO 2 values given by the 4-wavelength EQUANOX™ 7600 device (Nonin Medical Inc., Plymouth, Mn) and O3™ device (Masimo Corporation, Irvine, CA). Twenty adult patients scheduled for conventional elective cardiac surgery with cardiopulmonary bypass over a 4-month period were included after local Ethics Committee approval. For each patient, 2 NIRS sensors (EQUANOX and O3) were placed over the medial part of the forearm. Thirteen couples of measurements were performed at predefined intraoperative time points. We compared 260 couples of absolute intraoperative rSO 2 values. No significant difference was found between both monitors: EQUANOX median rSO 2 60% (95% CI 57-62) versus O3 median rSO 2 62% (95% CI 61-64), P = 0.103. Bias was 4.0% and limits of agreement were ±26.3%. Significant correlations were evidenced between EQUANOX and O3 rSO 2 absolute values: rho = 0.758 (95% CI 0.701-0.806), P < 0.0001, and rSO 2 percent maximum difference versus baseline: rho = 0.582 (95% CI 0.188-0.815), P = 0.007. While absolute values of rSO 2 given by both devices were equivalent and well correlated, the clinical agreement is probably not acceptable, meaning that EQUANOX and O3 are not interchangeable in routine practice.

NIR-to-NIR Deep Penetrating Nanoplatforms Y2O3:Nd3+/Yb3+@SiO2@Cu2S toward Highly Efficient Photothermal Ablation.

PubMed

Zhang, Zhiyu; Suo, Hao; Zhao, Xiaoqi; Sun, Dan; Fan, Li; Guo, Chongfeng

2018-05-02

A difunctional nano-photothermal therapy (PTT) platform with near-infrared excitation to near-infrared emission (NIR-to-NIR) was constructed through core-shell structures Y 2 O 3 :Nd 3+ /Yb 3+ @SiO 2 @Cu 2 S (YRSC), in which the core Y 2 O 3 :Nd 3+ /Yb 3+ and shell Cu 2 S play the role of bioimaging and photothermal conversion function, respectively. The structure and composition of the present PTT agents (PTAs) were characterized by powder X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectra. The NIR emissions of samples in the biological window area were measured by photoluminescence spectra under the excitation of 808 nm laser; further, the penetration depth of NIR emission at different wavelengths in biological tissue was also demonstrated by comparing with visible (vis) emission from Y 2 O 3 :Yb 3+ /Er 3+ @SiO 2 @Cu 2 S and NIR emission from YRSC through different injection depths in pork muscle tissues. The photo-thermal conversion effects were achieved through the outer ultrasmall Cu 2 S nanoparticles simultaneously absorb NIR light emission from the core Y 2 O 3 :Nd 3+/ Yb 3+ and the 808 nm excitation source to generate heat. Further, the heating effect of YRSC nanoparticles was confirmed by thermal imaging and ablation of YRSC to Escherichia coli and human hepatoma (HepG-2) cells. Results indicate that the YRSC has potential applications in PTT and NIR imaging in biological tissue.

Near-infrared localized surface plasmon resonance of self-growing W-doped VO2 nanoparticles at room temperature

NASA Astrophysics Data System (ADS)

Nishikawa, Kazutaka; Kishida, Yoshihiro; Ito, Kota; Tamura, Shin-ichi; Takeda, Yasuhiko

2017-11-01

Nanoparticles (NPs) of vanadium dioxide (VO2) in the metal state exhibit localized surface plasmon resonance (LSPR) at 1200-1600 nm, which fills the gap between the absorption ranges of silicon and the LSPR of conventional transparent conductor NPs (ZnO:Al, In2O3:Sn, etc.). However, two issues of the lithographic process for NP formation and the metal-insulator transition temperature (69 °C) higher than room temperature have made it difficult to use VO2 NPs for applications such as energy conversion devices, near infrared (NIR) light detectors, and bio-therapy. In this study, we developed a self-growing process for tungsten (W)-doped VO2 NPs that are in the metal state at room temperature, using sputter deposition and post-lamp annealing. The changes in the LSPR peak wavelengths with the NP size were well controlled by changing the deposited film thickness and oxygen pressure during the post-annealing treatment. The presented results resolve the difficulties of using the metal-insulator transition material VO2 for practical NIR utilization.

Photothermal therapy of Lewis lung carcinoma in mice using gold nanoshells on carboxylated polystyrene spheres

NASA Astrophysics Data System (ADS)

Liu, Huiyu; Chen, Dong; Tang, Fangqiong; Du, Gangjun; Li, Linlin; Meng, Xianwei; Liang, Wei; Zhang, Yangde; Teng, Xu; Li, Yi

2008-11-01

A new approach towards the design of gold nanoshells on carboxylated polystyrene spheres (GNCPSs) is reported here. Gold nanoshells were self-assembled on the surface of carboxylated polystyrene spheres by a seed growth method. Chitosan (CHI) was used as a functional agent of carboxylated polystyrene spheres for attaching gold seeds. The surface plasmon resonance (SPR) peak of GNCPSs can be tuned, greatly redshifted, over a broad spectral range including the near-infrared (NIR) wavelength region, which provides maximal penetration of light through tissue. Irradiation of GNCPSs at their peak extinction coefficient results in the conversion of light to heat energy that produces a local rise in temperature. Our study revealed that the Lewis lung carcinoma (LLC) in mice treated with GNCPSs exposed to a low dose of NIR light (808 nm, 4 W cm-2) induced irreversible tissue damage. The tumor volumes of the treatment group by GNCPSs were significantly lower than those of control groups, with an average inhibition rate over 55% (P<0.005). This study proves that GNCPSs are promising in plasmonic photothermal tumor therapy.

Noninvasive assessment of diabetic foot ulcers with diffuse photon density wave methodology: pilot human study

NASA Astrophysics Data System (ADS)

Papazoglou, Elisabeth S.; Neidrauer, Michael; Zubkov, Leonid; Weingarten, Michael S.; Pourrezaei, Kambiz

2009-11-01

A pilot human study is conducted to evaluate the potential of using diffuse photon density wave (DPDW) methodology at near-infrared (NIR) wavelengths (685 to 830 nm) to monitor changes in tissue hemoglobin concentration in diabetic foot ulcers. Hemoglobin concentration is measured by DPDW in 12 human wounds for a period ranging from 10 to 61 weeks. In all wounds that healed completely, gradual decreases in optical absorption coefficient, oxygenated hemoglobin concentration, and total hemoglobin concentration are observed between the first and last measurements. In nonhealing wounds, the rates of change of these properties are nearly zero or slightly positive, and a statistically significant difference (p<0.05) is observed in the rates of change between healing and nonhealing wounds. Differences in the variability of DPDW measurements over time are observed between healing and nonhealing wounds, and this variance may also be a useful indicator of nonhealing wounds. Our results demonstrate that DPDW methodology with a frequency domain NIR device can differentiate healing from nonhealing diabetic foot ulcers, and indicate that it may have clinical utility in the evaluation of wound healing potential.

Evaluating Field Spectrometer Performance with Transmission Standards: Examples from the USGS Spectral Library and Research Databases

NASA Astrophysics Data System (ADS)

Hoefen, T. M.; Kokaly, R. F.; Swayze, G. A.; Livo, K. E.

2015-12-01

Collection of spectroscopic data has expanded with the development of field-portable spectrometers. The most commonly available spectrometers span one or several wavelength ranges: the visible (VIS) and near-infrared (NIR) region from approximately 400 to 1000 nm, and the shortwave infrared (SWIR) region from approximately 1000-2500 nm. Basic characteristics of spectrometer performance are the wavelength position and bandpass of each channel. Bandpass can vary across the wavelength coverage of an instrument, due to spectrometer design and detector materials. Spectrometer specifications can differ from one instrument to the next for a given model and between manufacturers. The USGS Spectroscopy Lab in Denver has developed a simple method to evaluate field spectrometer wavelength accuracy and bandpass values using transmission measurements of materials with intense, narrow absorption features, including Mylar* plastic, praseodymium-doped glass, and National Institute of Standards and Technology Standard Reference Material 2035. The evaluation procedure has been applied in laboratory and field settings for 19 years and used to detect deviations from cited manufacturer specifications. Tracking of USGS spectrometers with transmission standards has revealed several instances of wavelength shifts due to wear in spectrometer components. Since shifts in channel wavelengths and differences in bandpass between instruments can impact the use of field spectrometer data to calibrate and analyze imaging spectrometer data, field protocols to measure wavelength standards can limit data loss due to spectrometer degradation. In this paper, the evaluation procedure will be described and examples of observed wavelength shifts during a spectrometer field season will be presented. The impact of changing wavelength and bandpass characteristics on spectral measurements will be demonstrated and implications for spectral libraries will be discussed. *Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Simple aerosol correction technique based on the spectral relationships of the aerosol multiple-scattering reflectances for atmospheric correction over the oceans.

PubMed

Ahn, Jae-Hyun; Park, Young-Je; Kim, Wonkook; Lee, Boram

2016-12-26

An estimation of the aerosol multiple-scattering reflectance is an important part of the atmospheric correction procedure in satellite ocean color data processing. Most commonly, the utilization of two near-infrared (NIR) bands to estimate the aerosol optical properties has been adopted for the estimation of the effects of aerosols. Previously, the operational Geostationary Color Ocean Imager (GOCI) atmospheric correction scheme relies on a single-scattering reflectance ratio (SSE), which was developed for the processing of the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) data to determine the appropriate aerosol models and their aerosol optical thicknesses. The scheme computes reflectance contributions (weighting factor) of candidate aerosol models in a single scattering domain then spectrally extrapolates the single-scattering aerosol reflectance from NIR to visible (VIS) bands using the SSE. However, it directly applies the weight value to all wavelengths in a multiple-scattering domain although the multiple-scattering aerosol reflectance has a non-linear relationship with the single-scattering reflectance and inter-band relationship of multiple scattering aerosol reflectances is non-linear. To avoid these issues, we propose an alternative scheme for estimating the aerosol reflectance that uses the spectral relationships in the aerosol multiple-scattering reflectance between different wavelengths (called SRAMS). The process directly calculates the multiple-scattering reflectance contributions in NIR with no residual errors for selected aerosol models. Then it spectrally extrapolates the reflectance contribution from NIR to visible bands for each selected model using the SRAMS. To assess the performance of the algorithm regarding the errors in the water reflectance at the surface or remote-sensing reflectance retrieval, we compared the SRAMS atmospheric correction results with the SSE atmospheric correction using both simulations and in situ match-ups with the GOCI data. From simulations, the mean errors for bands from 412 to 555 nm were 5.2% for the SRAMS scheme and 11.5% for SSE scheme in case-I waters. From in situ match-ups, 16.5% for the SRAMS scheme and 17.6% scheme for the SSE scheme in both case-I and case-II waters. Although we applied the SRAMS algorithm to the GOCI, it can be applied to other ocean color sensors which have two NIR wavelengths.

Utilization of functional near infrared spectroscopy for non-invasive evaluation

NASA Astrophysics Data System (ADS)

Halim, A. A. A.; Laili, M. H.; Aziz, N. A.; Laili, A. R.; Salikin, M. S.; Rusop, M.

2016-07-01

The goal of this brief review is to report the techniques of functional near infrared spectroscopy for non-invasive evaluation in human study. The development of functional near infrared spectroscopy (fNIRS) technologies has advanced quantification signal using multiple wavelength and detector to solve the propagation of light inside the tissues including the absorption, scattering coefficient and to define the light penetration into tissues multilayers. There are a lot of studies that demonstrate signal from fNIRS which can be used to evaluate the changes of oxygenation level and measure the limitation of muscle performance in human brain and muscle tissues. Comprehensive reviews of diffuse reflectance based on beer lambert law theory were presented in this paper. The principle and development of fNIRS instrumentation is reported in detail.

Detection of fatty product falsifications using a portable near infrared spectrometer

NASA Astrophysics Data System (ADS)

Kalinin, A. V.; Krasheninnikov, V. N.

2017-01-01

Spreading sales of counterfeited fatty-oil foods leads to a development of portable and operational analyzer of typical fatty acids (FA) which may be a near infrared (NIR) spectrometer. In this work the calibration models for prediction of named FA were built with the spectra of FT-NIR spectrometer for different absorption bands of the FA. The best parameters were obtained for the wavelength sub-band 1.0-1.8 μ, which includes the 2nd and 3rd overtones of C-H stretching vibrations (near 1.7 and 1.2 μ) and the combination band (1.42 μ). Applicability of the portable spectrometer based on linear NIR array photosensor for the quality analysis of spread, butter and fish oil by the typical FA has been tested.

Improving NIR snow pit stratigraphy observations by introducing a controlled NIR light source

NASA Astrophysics Data System (ADS)

Dean, J.; Marshall, H.; Rutter, N.; Karlson, A.

2013-12-01

Near-infrared (NIR) photography in a prepared snow pit measures mm-/grain-scale variations in snow structure, as reflectivity is strongly dependent on microstructure and grain size at the NIR wavelengths. We explore using a controlled NIR light source to maximize signal to noise ratio and provide uniform incident, diffuse light on the snow pit wall. NIR light fired from the flash is diffused across and reflected by an umbrella onto the snow pit; the lens filter transmits NIR light onto the spectrum-modified sensor of the DSLR camera. Lenses are designed to refract visible light properly, not NIR light, so there must be a correction applied for the subsequent NIR bright spot. To avoid interpolation and debayering algorithms automatically performed by programs like Adobe's Photoshop on the images, the raw data are analyzed directly in MATLAB. NIR image data show a doubling of the amount of light collected in the same time for flash over ambient lighting. Transitions across layer boundaries in the flash-lit image are detailed by higher camera intensity values than ambient-lit images. Curves plotted using median intensity at each depth, normalized to the average profile intensity, show a separation between flash- and ambient-lit images in the upper 10-15 cm; the ambient-lit image curve asymptotically approaches the level of the flash-lit image curve below 15cm. We hypothesize that the difference is caused by additional ambient light penetrating the upper 10-15 cm of the snowpack from above and transmitting through the wall of the snow pit. This indicates that combining NIR ambient and flash photography could be a powerful technique for studying penetration depth of radiation as a function of microstructure and grain size. The NIR flash images do not increase the relative contrast at layer boundaries; however, the flash more than doubles the amount of recorded light and controls layer noise as well as layer boundary transition noise.

Comparative investigation of methods to determine the group velocity dispersion of an endlessly single-mode photonic crystal fiber

NASA Astrophysics Data System (ADS)

Baselt, Tobias; Popp, Tobias; Nelsen, Bryan; Lasagni, Andrés. Fabián.; Hartmann, Peter

2017-05-01

Endlessly single-mode fibers, which enable single mode guidance over a wide spectral range, are indispensable in the field of fiber technology. A two-dimensional photonic crystal with a silica central core and a micrometer-spaced hexagonal array of air holes is an established method to achieve endless single-mode guidance. There are two possible ways to determine the dispersion: measurement and calculation. We calculate the group velocity dispersion GVD based on the measurement of the fiber structure parameters, the hole diameter and the pitch of a presumed homogeneous hexagonal array and compare the calculation with two methods to measure the wavelength-dependent time delay. We measure the time delay on a three hundred meter test fiber with a homemade supercontinuum light source, a set of bandpass filters and a fast detector and compare the results with a white light interferometric setup. To measure the dispersion of optical fibers with high accuracy, a time-frequency-domain setup based on a Mach-Zehnder interferometer is used. The experimental setup allows the determination of the wavelength dependent differential group delay of light travelling through a thirty centimeter piece of test fiber in the wavelength range from VIS to NIR. The determination of the GVD using different methods enables the evaluation of the individual methods for characterizing the endlessly single-mode fiber.

An optical water type framework for selecting and blending retrievals from bio-optical algorithms in lakes and coastal waters.

PubMed

Moore, Timothy S; Dowell, Mark D; Bradt, Shane; Verdu, Antonio Ruiz

2014-03-05

Bio-optical models are based on relationships between the spectral remote sensing reflectance and optical properties of in-water constituents. The wavelength range where this information can be exploited changes depending on the water characteristics. In low chlorophyll- a waters, the blue/green region of the spectrum is more sensitive to changes in chlorophyll- a concentration, whereas the red/NIR region becomes more important in turbid and/or eutrophic waters. In this work we present an approach to manage the shift from blue/green ratios to red/NIR-based chlorophyll- a algorithms for optically complex waters. Based on a combined in situ data set of coastal and inland waters, measures of overall algorithm uncertainty were roughly equal for two chlorophyll- a algorithms-the standard NASA OC4 algorithm based on blue/green bands and a MERIS 3-band algorithm based on red/NIR bands-with RMS error of 0.416 and 0.437 for each in log chlorophyll- a units, respectively. However, it is clear that each algorithm performs better at different chlorophyll- a ranges. When a blending approach is used based on an optical water type classification, the overall RMS error was reduced to 0.320. Bias and relative error were also reduced when evaluating the blended chlorophyll- a product compared to either of the single algorithm products. As a demonstration for ocean color applications, the algorithm blending approach was applied to MERIS imagery over Lake Erie. We also examined the use of this approach in several coastal marine environments, and examined the long-term frequency of the OWTs to MODIS-Aqua imagery over Lake Erie.

An optical water type framework for selecting and blending retrievals from bio-optical algorithms in lakes and coastal waters

PubMed Central

Moore, Timothy S.; Dowell, Mark D.; Bradt, Shane; Verdu, Antonio Ruiz

2014-01-01

Bio-optical models are based on relationships between the spectral remote sensing reflectance and optical properties of in-water constituents. The wavelength range where this information can be exploited changes depending on the water characteristics. In low chlorophyll-a waters, the blue/green region of the spectrum is more sensitive to changes in chlorophyll-a concentration, whereas the red/NIR region becomes more important in turbid and/or eutrophic waters. In this work we present an approach to manage the shift from blue/green ratios to red/NIR-based chlorophyll-a algorithms for optically complex waters. Based on a combined in situ data set of coastal and inland waters, measures of overall algorithm uncertainty were roughly equal for two chlorophyll-a algorithms—the standard NASA OC4 algorithm based on blue/green bands and a MERIS 3-band algorithm based on red/NIR bands—with RMS error of 0.416 and 0.437 for each in log chlorophyll-a units, respectively. However, it is clear that each algorithm performs better at different chlorophyll-a ranges. When a blending approach is used based on an optical water type classification, the overall RMS error was reduced to 0.320. Bias and relative error were also reduced when evaluating the blended chlorophyll-a product compared to either of the single algorithm products. As a demonstration for ocean color applications, the algorithm blending approach was applied to MERIS imagery over Lake Erie. We also examined the use of this approach in several coastal marine environments, and examined the long-term frequency of the OWTs to MODIS-Aqua imagery over Lake Erie. PMID:24839311

New techniques for laser beam atmospheric extinction measurements from manned and unmanned aerospace vehicles

NASA Astrophysics Data System (ADS)

Sabatini, Roberto; Richardson, Mark

2013-03-01

Novel techniques for laser beam atmospheric extinction measurements, suitable for several air and space platform applications, are presented in this paper. Extinction measurements are essential to support the engineering development and the operational employment of a variety of aerospace electro-optical sensor systems, allowing calculation of the range performance attainable with such systems in current and likely future applications. Such applications include ranging, weaponry, Earth remote sensing and possible planetary exploration missions performed by satellites and unmanned flight vehicles. Unlike traditional LIDAR methods, the proposed techniques are based on measurements of the laser energy (intensity and spatial distribution) incident on target surfaces of known geometric and reflective characteristics, by means of infrared detectors and/or infrared cameras calibrated for radiance. Various laser sources can be employed with wavelengths from the visible to the far infrared portions of the spectrum, allowing for data correlation and extended sensitivity. Errors affecting measurements performed using the proposed methods are discussed in the paper and algorithms are proposed that allow a direct determination of the atmospheric transmittance and spatial characteristics of the laser spot. These algorithms take into account a variety of linear and non-linear propagation effects. Finally, results are presented relative to some experimental activities performed to validate the proposed techniques. Particularly, data are presented relative to both ground and flight trials performed with laser systems operating in the near infrared (NIR) at λ= 1064 nm and λ= 1550 nm. This includes ground tests performed with 10 Hz and 20 KHz PRF NIR laser systems in a large variety of atmospheric conditions, and flight trials performed with a 10 Hz airborne NIR laser system installed on a TORNADO aircraft, flying up to altitudes of 22,000 ft.

Novel atmospheric extinction measurement techniques for aerospace laser system applications

NASA Astrophysics Data System (ADS)

Sabatini, Roberto; Richardson, Mark

2013-01-01

Novel techniques for laser beam atmospheric extinction measurements, suitable for manned and unmanned aerospace vehicle applications, are presented in this paper. Extinction measurements are essential to support the engineering development and the operational employment of a variety of aerospace electro-optical sensor systems, allowing calculation of the range performance attainable with such systems in current and likely future applications. Such applications include ranging, weaponry, Earth remote sensing and possible planetary exploration missions performed by satellites and unmanned flight vehicles. Unlike traditional LIDAR methods, the proposed techniques are based on measurements of the laser energy (intensity and spatial distribution) incident on target surfaces of known geometric and reflective characteristics, by means of infrared detectors and/or infrared cameras calibrated for radiance. Various laser sources can be employed with wavelengths from the visible to the far infrared portions of the spectrum, allowing for data correlation and extended sensitivity. Errors affecting measurements performed using the proposed methods are discussed in the paper and algorithms are proposed that allow a direct determination of the atmospheric transmittance and spatial characteristics of the laser spot. These algorithms take into account a variety of linear and non-linear propagation effects. Finally, results are presented relative to some experimental activities performed to validate the proposed techniques. Particularly, data are presented relative to both ground and flight trials performed with laser systems operating in the near infrared (NIR) at λ = 1064 nm and λ = 1550 nm. This includes ground tests performed with 10 Hz and 20 kHz PRF NIR laser systems in a large variety of atmospheric conditions, and flight trials performed with a 10 Hz airborne NIR laser system installed on a TORNADO aircraft, flying up to altitudes of 22,000 ft.

Long-wavelength analyte-sensitive luminescent probes and optical (bio)sensors

PubMed Central

Staudinger, Christoph; Borisov, Sergey M

2016-01-01

Long-wavelength luminescent probes and sensors become increasingly popular. They offer the advantage of lower levels of autofluorescence in most biological probes. Due to high penetration depth and low scattering of red and NIR light such probes potentially enable in vivo measurements in tissues and some of them have already reached a high level of reliability required for such applications. This review focuses on the recent progress in development and application of long-wavelength analyte-sensitive probes which can operate both reversibly and irreversibly. Photophysical properties, sensing mechanisms, advantages and limitations of individual probes are discussed. PMID:27134748

Optimal wavelength selection for noncontact reflection photoplethysmography

NASA Astrophysics Data System (ADS)

Corral Martinez, Luis F.; Paez, Gonzalo; Strojnik, Marija

2011-08-01

In this work, we obtain backscattered signals from human forehead for wavelengths from 380 to 980 nm. The results reveal bands with strong pulsatile signals that carry useful information. We describe those bands as the most suitable wavelengths in the visible and NIR regions from which heart and respiratory rate parameters can be derived using long distance non-contact reflection photoplethysmography analysis. The latter results show the feasibility of a novel technique for remotely detection of vital signs in humans. This technique, which may include morphological analysis or maps of tissue oxygenation, is a further step to real non-invasive remote monitoring of patients.

Near infrared fluorescent biliproteins generated from bacteriophytochrome AphB of Nostoc sp. PCC 7120.

PubMed

Yuan, Che; Li, Hui-Zhen; Tang, Kun; Gärtner, Wolfgang; Scheer, Hugo; Zhou, Ming; Zhao, Kai-Hong

2016-04-01

The genome of the cyanobacterium Nostoc sp. PCC 7120 encodes a large number of putative bacteriophytochrome and cyanobacteriochrome photoreceptors that, due to their long-wavelength absorption and fluorescence emission, might serve as fluorescent tags in intracellular investigations. We show that the PAS-GAF domain of the bacteriophytochrome, AphB, binds biliverdin covalently and exhibits, besides its reversible photochemistry, a moderate fluorescence in the near infrared (NIR) spectral region. It was selected for further increasing the brightness while retaining the NIR fluorescence. In the first step, amino acids assumed to improve fluorescence were selectively mutated. The resulting variants were then subjected to several rounds of random mutagenesis and screened for enhanced fluorescence in the NIR. The brightness of optimized PAS-GAF variants increased more than threefold compared to that of wt AphB(1-321), with only insignificant spectral shifts (Amax around 695 nm, and Fmax around 720 nm). In general, the brightness increases with decreasing wavelengths, which allows for a selection of the fluorophore depending on the optical properties of the tissue. A spectral heterogeneity was observed when residue His260, located in close proximity to the chromophore, was mutated to Tyr, emphasizing the strong effects of the environment on the electronic properties of the bound biliverdin chromophore.

Host sensitized near-infrared emission in Nd3+ doped different alkaline-sodium-phosphate phosphors

NASA Astrophysics Data System (ADS)

Balakrishna, A.; Swart, H. C.; Kroon, R. E.; Ntwaeaborwa, O. M.

2018-04-01

Near-infrared (NIR) emitting phosphors of different alkaline based sodium-phosphate (MNa[PO4], where M = Mg, Ca, Sr and Ba were prepared by a conventional solution combustion method with fixed doping concentration of Nd3+ (1.0 mol%). The phosphors were characterized by powder X-ray diffraction, field emission scanning electron microscope, Fourier transform infrared spectroscopy, UV-vis spectroscopy and fluorescent spectrophotometry. The optical properties including reflectance, excitation and emission were investigated. The excitation spectra of the phosphors were characterized by a broadband extending from 450 to 900 nm. Upon excitation with a wavelength of 580 nm, the phosphor emits intensely infrared region at 872 nm, 1060 nm and 1325 nm which correspond to the 4F3/2 → 4I9/2, 4F3/2 → 4I11/2 and 4F3/2 → 4I13/2 transitions of Nd3+ ions and were found to vary for the different hosts. The strongest emission wavelength reaches 1060 nm. The most intense emission of Nd3+ was observed from Ca2+ incorporated host. The down conversion emissions of the material fall in the NIR region suggesting that the prepared phosphors have potential application in the development of photonic devices emitting in the NIR.

Lanthanide‐Doped Upconversion Nanoparticles: Emerging Intelligent Light‐Activated Drug Delivery Systems

PubMed Central

Bagheri, Ali; Arandiyan, Hamidreza

2016-01-01

The development of drug delivery systems (DDSs) using near infrared (NIR) light and upconversion nanoparticles (UCNPs) has generated intensive interest over the past five years. These NIR‐initiated DDSs not only offer a high degree of spatial and temporal determination of therapeutic release but also provide precise control over the released dosage. Furthermore, these nanoplatforms confer several advantages over conventional light‐based DDSs—NIR offers better tissue penetration depth and a reduced risk of cellular photo‐damage caused by exposure to light at high‐energy wavelengths (e.g., ultraviolet light, <400 nm). The development of DDSs that can be activated by low intensity NIR illumination is highly desirable to avoid exposing living tissues to excessive heat that can limit the in vivo application of these DDSs. This encompasses research in three directions: (i) enhancing the quantum yield of the UCNPs; (ii) incorporation of photo‐responsive materials with red‐shifted absorptions into the UCNPs; and (iii) tuning the UCNPs excitation wavelength. This review focuses on recent advances in the development of NIR‐initiated DDS, with emphasis on the use of photo‐responsive compounds and polymeric materials conjugated onto UCNPs. The challenges that limit UCNPs clinical applications, alongside with the aforementioned techniques that have emerged to overcome these limitations, are highlighted. PMID:27818904

Assessment of the dynamics of human glymphatic system by near-infrared spectroscopy.

PubMed

Myllylä, Teemu; Harju, Markus; Korhonen, Vesa; Bykov, Alexander; Kiviniemi, Vesa; Meglinski, Igor

2017-08-12

Fluctuations in brain water content has attracted increasing interest, particularly as regards studies of the glymphatic system, which is connected with the complex organization of dural lymphatic vessels, responsible for cleaning tissue. Disturbances of glymphatic circulation are associated with several brain disorders, including dementia. This article introduces an approach to noninvasive measurement of water dynamics in the human brain utilizing near-infrared spectroscopy (NIRS). We demonstrate the possibility to sense dynamic variations of water content between the skull and grey matter, for instance, in the subarachnoid space. Measured fluctuations in water content, especially in the cerebrospinal fluid (CSF), are assumed to be correlated with the dynamics of glymphatic circulation. The sampling volume for the NIRS optode was estimated by Monte Carlo modelling for the wavelengths of 660, 740, 830 and 980 nm. In addition, using combinations of these wavelengths, this article presents the calculation models for quantifying water and haemodynamics. The presented NIRS technique allows long-term functional brain monitoring, including sleeping time. Furthermore, it is used in combination with different magnetic neuroimaging techniques, particularly magnetic resonance encephalography. Using the combined setup, we report the preliminary results on the interaction between CSF and blood oxygen level-dependent fluctuations. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Continuous correction of differential path length factor in near-infrared spectroscopy

PubMed Central

Moore, Jason H.; Diamond, Solomon G.

2013-01-01

Abstract. In continuous-wave near-infrared spectroscopy (CW-NIRS), changes in the concentration of oxyhemoglobin and deoxyhemoglobin can be calculated by solving a set of linear equations from the modified Beer-Lambert Law. Cross-talk error in the calculated hemodynamics can arise from inaccurate knowledge of the wavelength-dependent differential path length factor (DPF). We apply the extended Kalman filter (EKF) with a dynamical systems model to calculate relative concentration changes in oxy- and deoxyhemoglobin while simultaneously estimating relative changes in DPF. Results from simulated and experimental CW-NIRS data are compared with results from a weighted least squares (WLSQ) method. The EKF method was found to effectively correct for artificially introduced errors in DPF and to reduce the cross-talk error in simulation. With experimental CW-NIRS data, the hemodynamic estimates from EKF differ significantly from the WLSQ (p<0.001). The cross-correlations among residuals at different wavelengths were found to be significantly reduced by the EKF method compared to WLSQ in three physiologically relevant spectral bands 0.04 to 0.15 Hz, 0.15 to 0.4 Hz and 0.4 to 2.0 Hz (p<0.001). This observed reduction in residual cross-correlation is consistent with reduced cross-talk error in the hemodynamic estimates from the proposed EKF method. PMID:23640027

Continuous correction of differential path length factor in near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Talukdar, Tanveer; Moore, Jason H.; Diamond, Solomon G.

2013-05-01

In continuous-wave near-infrared spectroscopy (CW-NIRS), changes in the concentration of oxyhemoglobin and deoxyhemoglobin can be calculated by solving a set of linear equations from the modified Beer-Lambert Law. Cross-talk error in the calculated hemodynamics can arise from inaccurate knowledge of the wavelength-dependent differential path length factor (DPF). We apply the extended Kalman filter (EKF) with a dynamical systems model to calculate relative concentration changes in oxy- and deoxyhemoglobin while simultaneously estimating relative changes in DPF. Results from simulated and experimental CW-NIRS data are compared with results from a weighted least squares (WLSQ) method. The EKF method was found to effectively correct for artificially introduced errors in DPF and to reduce the cross-talk error in simulation. With experimental CW-NIRS data, the hemodynamic estimates from EKF differ significantly from the WLSQ (p<0.001). The cross-correlations among residuals at different wavelengths were found to be significantly reduced by the EKF method compared to WLSQ in three physiologically relevant spectral bands 0.04 to 0.15 Hz, 0.15 to 0.4 Hz and 0.4 to 2.0 Hz (p<0.001). This observed reduction in residual cross-correlation is consistent with reduced cross-talk error in the hemodynamic estimates from the proposed EKF method.

Three-dimensional Ag2O/Bi5O7I p-n heterojunction photocatalyst harnessing UV-vis-NIR broad spectrum for photodegradation of organic pollutants.

PubMed

Chen, Yannan; Zhu, Gangqiang; Hojamberdiev, Mirabbos; Gao, Jianzhi; Zhu, Runliang; Wang, Chenghui; Wei, Xiumei; Liu, Peng

2018-02-15

Ag 2 O nanoparticles-loaded Bi 5 O 7 I microspheres forming a three dimensional Ag 2 O/Bi 5 O 7 I p-n heterojunction photocatalyst with wide-spectrum response were synthesized in this study. The results of transmission electron microscopy observations revealed that the Ag 2 O nanoparticles with the diameter of ca. 10-20nm were distributed on the surfaces of Bi 5 O 7 I nanosheets. The as-synthesized Ag 2 O/Bi 5 O 7 I exhibited an excellent wide-spectrum response to wavelengths ranging from ultraviolet (UV) to near-infrared (NIR), indicating its potential for effective utilization of solar energy. Compared with pure Bi 5 O 7 I, the Ag 2 O/Bi 5 O 7 I composite also demonstrated excellent photocatalytic activity for the degradation of Bisphenol A and phenol in aqueous solution under visible LED light irradiation. Among samples, the 20% Ag 2 O/Bi 5 O 7 I composite photocatalyst showed the highest photocatalytic activity for the degradation of Bisphenol A and phenol in aqueous solution. In addition, the 20% Ag 2 O/Bi 5 O 7 I composite also exhibited a photocatalytic activity for the degradation of Bisphenol A under NIR light irradiation. The improved photocatalytic activity is attributed to the formation of a p-n heterojunction between Ag 2 O and Bi 5 O 7 I, allowing the efficient utilization of solar energy (from UV to NIR) and high separation efficiency of photogenerated electron-hole pairs. The present work is desirable to explore a possible avenue for the full utilization of solar energy. Copyright © 2017 Elsevier B.V. All rights reserved.

Signal processing for a single detector MOEMS based NIR micro spectrometer

NASA Astrophysics Data System (ADS)

Heberer, Andreas; Grüger, Heinrich; Zimmer, Fabian; Schenk, Harald; Kenda, Andreas; Frank, Albert; Scherf, Werner

2005-10-01

The examination of spectra in the NIR range is necessary for applications like process control, element analysis or medical systems. Typically integrated NIR spectrometers are based on optical setups with diffraction grating and detector arrays. The main disadvantage is price and availability of NIR array InGaAs-based detectors. The implementation of a scanning grating chip realized in a MOEMS technology which integrates the diffractive element makes it possible to detect spectra with single detectors time resolved. Either simple InGaAs photodiodes or cooled detectors may be used. The set up is a shrinked Czerny-Turner spectrometer. The light is coupled in by an optical fibre. After focussing the light passes the scanning grating moving at 150-500 Hz in a sinusoidal way. There it is split off in the different wavelength, the monochrome intensity is caught by a second mirror and led to the detector. The detector signal is amplified by a transimpedance stage and converted to digital with 12 bit resolution. The main part of the signal processing is done by a digital signal processor, which is used to unfold the sinusoidal position and calculate the final spectra. The data rate can be up to 3 MHz, then a spectrum is acquired every 2ms by using a 500Hz Mirror. Using the DSP, the spectrometer can operate autarkic without any PC. Then the spectrum is display on a 160 x 80 pixel graphic LCD. A keypad is used to control the functions. For communication a USB port is included, additional interfaces can be realized by a 16-pin expansion port, which is freely programmable, by the system firmware.

Reflectance Spectra Diversity of Silica-Rich Materials: Sensitivity to Environment and Implications for Detections on Mars

NASA Technical Reports Server (NTRS)

Rice, M. S.; Cloutis, E. A.; Bell, J. F., III; Bish, D. L.; Horgan, B. H.; Mertzman, S. A.; Craig, M. A.; Renault, R. W.; Gautason, B.; Mountain, B.

2013-01-01

Hydrated silica-rich materials have recently been discovered on the surface of Mars by the Mars Exploration Rover (MER) Spirit, the Mars Reconnaissance Orbiter (MRO) Compact Reconnaissance Imaging Spectrometer for Mars (CRISM), and the Mars Express Observatoire pour la Mineralogie, l'Eau, les Glaces, et l'Activite'(OMEGA) in several locations. Having been interpreted as hydrothermal deposits and aqueous alteration products, these materials have important implications for the history of water on the martian surface. Spectral detections of these materials in visible to near infrared (Vis NIR) wavelengths have been based on a H2O absorption feature in the 934-1009 nm region seen with Spirit s Pancam instrument, and on SiOH absorption features in the 2.21-2.26 micron range seen with CRISM. Our work aims to determine how the spectral reflectance properties of silica-rich materials in Vis NIR wavelengths vary as a function of environmental conditions and formation. Here we present laboratory reflectance spectra of a diverse suite of silica-rich materials (chert, opal, quartz, natural sinters and synthetic silica) under a range of grain sizes and temperature, pressure, and humidity conditions. We find that the H2O content and form of H2O/OH present in silica-rich materials can have significant effects on their Vis NIR spectra. Our main findings are that the position of the approx.1.4 microns OH feature and the symmetry of the approx.1.9 microns feature can be used to discern between various forms of silica-rich materials, and that the ratio of the approx.2.2 microns (SiOH) and approx.1.9 microns (H2O) band depths can aid in distinguishing between silica phases (opal-A vs. opal-CT) and formation conditions (low vs. high temperature). In a case study of hydrated silica outcrops in Valles Marineris, we show that careful application of a modified version of these spectral parameters to orbital near-infrared spectra (e.g., from CRISM and OMEGA) can aid in characterizing the compositional diversity of silica-bearing deposits on Mars. We also discuss how these results can aid in the interpretation of silica detections on Mars made by the MER Panoramic Camera (Pancam) and Mars Science Laboratory (MSL) Mast-mounted Camera (Mastcam) instruments.

Analysis of ecstasy tablets: comparison of reflectance and transmittance near infrared spectroscopy.

PubMed

Schneider, Ralph Carsten; Kovar, Karl-Artur

2003-07-08

Calibration models for the quantitation of commonly used ecstasy substances have been developed using near infrared spectroscopy (NIR) in diffuse reflectance and in transmission mode by applying seized ecstasy tablets for model building and validation. The samples contained amphetamine, N-methyl-3,4-methylenedioxy-amphetamine (MDMA) and N-ethyl-3,4-methylenedioxy-amphetamine (MDE) in different concentrations. All tablets were analyzed using high performance liquid chromatography (HPLC) with diode array detection as reference method. We evaluated the performance of each NIR measurement method with regard to its ability to predict the content of each tablet with a low root mean square error of prediction (RMSEP). Best calibration models could be generated by using NIR measurement in transmittance mode with wavelength selection and 1/x-transformation of the raw data. The models build in reflectance mode showed higher RMSEPs using as data pretreatment, wavelength selection, 1/x-transformation and a second order Savitzky-Golay derivative with five point smoothing was applied to obtain the best models. To estimate the influence of inhomogeneities in the illegal tablets, a calibration of the destroyed, i.e. triturated samples was build and compared to the corresponding data of the whole tablets. The calibrations using these homogenized tablets showed lower RMSEPs. We can conclude that NIR analysis of ecstasy tablets in transmission mode is more suitable than measurement in diffuse reflectance to obtain quantification models for their active ingredients with regard to low errors of prediction. Inhomogeneities in the samples are equalized when measuring the tablets as powdered samples.

Improvements of VIIRS and MODIS Solar Diffuser and Lunar Calibration

NASA Technical Reports Server (NTRS)

Xiong, Xiaoxiong; Butler, James J.; Lei, Ning; Sun, Junqiang; Fulbright, Jon; Wang, Zhipeng; McIntire, Jeff; Angal, Amit Avinash

2013-01-01

Both VIIRS and MODIS instruments use solar diffuser (SD) and lunar observations to calibrate their reflective solar bands (RSB). A solar diffuser stability monitor (SDSM) is used to track the SD on-orbit degradation. On-orbit observations have shown similar wavelength-dependent SD degradation (larger at shorter VIS wavelengths) and SDSM detector response degradation (larger at longer NIR wavelengths) for both VIIRS and MODIS instruments. In general, the MODIS scan mirror has experienced more degradation in the VIS spectral region whereas the VIIRS rotating telescope assembly (RTA) mirrors have seen more degradation in the NIR and SWIR spectral region. Because of this wavelength dependent mirror degradation, the sensor's relative spectral response (RSR) needs to be modulated. Due to differences between the solar and lunar spectral irradiance, the modulated RSR could have different effects on the SD and lunar calibration. In this paper, we identify various factors that should be considered for the improvements of VIIRS and MODIS solar and lunar calibration and examine their potential impact. Specifically, we will characterize and assess the calibration impact due to SD and SDSM attenuation screen transmission (uncertainty), SD BRF uncertainty and onorbit degradation, SDSM detector response degradation, and modulated RSR resulting from the sensor's optics degradation. Also illustrated and discussed in this paper are the calibration strategies implemented in the VIIRS and MODIS SD and lunar calibrations and efforts that could be made for future improvements.

Spectral analysis of lunar analogue samples

NASA Astrophysics Data System (ADS)

Offringa, Marloes; Foing, Bernard

2016-04-01

Analyses of samples derived from terrestrial analogue sites are used to study lunar processes in their geological context (Foing, Stoker, Ehrenfreund, 2011). For this study samples from the volcanic region of the Eifel, Germany collected during field campaigns (Foing et al., 2010), are analyzed with a variety of spectrometers. The aim is to obtain a database of analyzed samples that could be used as a reference for future in situ measurements. Equipment used in the laboratory consists of a Fourier Transform Infrared (FTIR) spectrometer, an X-Ray Fluorescence (XRF) spectrometer, a Raman laser spectrometer, as well as UV-VIS and NIR reflectance spectrometers. The Raman, UV-VIS and NIR are also used in combination with the EXoGeoLab mock-up lander during field campaigns (Foing, Stoker, Ehrenfreund, 2011). Calibration of the UV-VIS and NIR reflectance spectrometers is the main focus of this research in order to obtain the clearest spectra. The calibration of the UV-VIS and NIR reflectance spectrometers requires the use of a good light source as well as suitable optical fibers to create a signal that covers the widest range in wavelengths available. To eliminate noise towards the edges of this range, multiple measurements are averaged and data is processed by dividing the signal by reference spectra. Calibration of the devices by creating a new dark and reference spectra has to take place after every sample measurement. In this way we take into account changes that occur in the signal due to the eating of the devices during the measurements. Moreover, the integration time is adjusted to obtain a clear signal without leading to oversaturation in the reflectance spectrum. The typical integration times for the UV-VIS reflectance spectrometer vary between 1 - 18 s, depending on the amount of daylight during experiments. For the NIR reflectance spectrometer the integration time resulting in the best signals is approximately 150 ms in combination with a broad spectrum light source. Together with taking an average over ±600 measurements per sample this leads to the best spectral signals that can be acquired with this set-up. Obtained spectra can be tested for accuracy by comparing them with stationary laboratory spectrometers such as the FTIR spectrometer. Future campaigns involving the employment of the spectrometers on the ExoGeoLab lander would prove the applicability of the equipment in the field.

On the prediction of threshold friction velocity of wind erosion using soil reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Li, Junran; Flagg, Cody; Okin, Gregory S.; Painter, Thomas H.; Dintwe, Kebonye; Belnap, Jayne

2015-12-01

Current approaches to estimate threshold friction velocity (TFV) of soil particle movement, including both experimental and empirical methods, suffer from various disadvantages, and they are particularly not effective to estimate TFVs at regional to global scales. Reflectance spectroscopy has been widely used to obtain TFV-related soil properties (e.g., moisture, texture, crust, etc.), however, no studies have attempted to directly relate soil TFV to their spectral reflectance. The objective of this study was to investigate the relationship between soil TFV and soil reflectance in the visible and near infrared (VIS-NIR, 350-2500 nm) spectral region, and to identify the best range of wavelengths or combinations of wavelengths to predict TFV. Threshold friction velocity of 31 soils, along with their reflectance spectra and texture were measured in the Mojave Desert, California and Moab, Utah. A correlation analysis between TFV and soil reflectance identified a number of isolated, narrow spectral domains that largely fell into two spectral regions, the VIS area (400-700 nm) and the short-wavelength infrared (SWIR) area (1100-2500 nm). A partial least squares regression analysis (PLSR) confirmed the significant bands that were identified by correlation analysis. The PLSR further identified the strong relationship between the first-difference transformation and TFV at several narrow regions around 1400, 1900, and 2200 nm. The use of PLSR allowed us to identify a total of 17 key wavelengths in the investigated spectrum range, which may be used as the optimal spectral settings for estimating TFV in the laboratory and field, or mapping of TFV using airborne/satellite sensors.

A discrimination model in waste plastics sorting using NIR hyperspectral imaging system.

PubMed

Zheng, Yan; Bai, Jiarui; Xu, Jingna; Li, Xiayang; Zhang, Yimin

2018-02-01

Classification of plastics is important in the recycling industry. A plastic identification model in the near infrared spectroscopy wavelength range 1000-2500 nm is proposed for the characterization and sorting of waste plastics using acrylonitrile butadiene styrene (ABS), polystyrene (PS), polypropylene (PP), polyethylene (PE), polyethylene terephthalate (PET), and polyvinyl chloride (PVC). The model is built by the feature wavelengths of standard samples applying the principle component analysis (PCA), and the accuracy, property and cross-validation of the model were analyzed. The model just contains a simple equation, center of mass coordinates, and radial distance, with which it is easy to develop classification and sorting software. A hyperspectral imaging system (HIS) with the identification model verified its practical application by using the unknown plastics. Results showed that the identification accuracy of unknown samples is 100%. All results suggested that the discrimination model was potential to an on-line characterization and sorting platform of waste plastics based on HIS. Copyright © 2017 Elsevier Ltd. All rights reserved.

Remote Sensing of Coastal and Inland Waters

NASA Astrophysics Data System (ADS)

De Keukelaere, L.; Sterckx, S.; Adriaensen, S.; Knaeps, E.

2016-02-01

The new generation of satellites (e.g. Landsat 8, HyspIRI, Sentinel 2 and Sentinel 3 …) contain sensors that enable monitoring at increased spatial and/or spectral resolution. This opens a wide range of new opportunities, amongst others improved observation of coastal and inland waters. Algorithms for the pre-processing of these images and the derivation of Level 2 products for these waters need to take into account the specific nature of these environments, with adjacency effects of the nearby land and complex interactions of the optially active substances with varying degrees of turbidity. Here a new atmospheric correction algorithm, OPERA, is presented which can deal with these highly complex environments and which is sensor generic. OPERA accounts for the contribution of adjacency effects and provides surface reflectances for both land and water targets. OPERA is extended with a level 2 water algorithm providing TSM and turbidity estimates for a wide variety of water types. The algorithm is based on a multi wavelength switching approach using shorter wavelengths in low turbid waters and long NIR and SWIR wavelengths for highly and extremely turbid waters. Results are shown for Landsat-8, Sentinel-2 and MERIS for a variety of scenes, validated with field aeronet and turbidity data.

Synthesis, growth, structural and optical studies of a novel organic Piperazine (bis) p-toluenesulfonate single crystal.

PubMed

Rekha, P; Peramaiyan, G; NizamMohideen, M; Kumar, R Mohan; Kanagadurai, R

2015-03-15

A novel organic single crystal of Piperazinium (bis) p-toluenesulfonate (PPTS) was grown by a slow evaporation solution growth technique. The structure of the grown crystal was determined using single crystal X-ray diffraction analysis. The PPTS crystal belongs to the triclinic crystal system with space group of P1¯. The presence of functional groups was confirmed by FTIR spectral analysis. The optical transmittance range and cut-off wavelength were identified by UV-vis-NIR spectral studies. The luminescent properties of PPTS crystal were investigated. The thermal behavior of PPTS crystal was studied by TG-DT analyses. Copyright © 2014 Elsevier B.V. All rights reserved.

Quantitative analysis of multi-component gas mixture based on AOTF-NIR spectroscopy

NASA Astrophysics Data System (ADS)

Hao, Huimin; Zhang, Yong; Liu, Junhua

2007-12-01

Near Infrared (NIR) spectroscopy analysis technology has attracted many eyes and has wide application in many domains in recent years because of its remarkable advantages. But the NIR spectrometer can only be used for liquid and solid analysis by now. In this paper, a new quantitative analysis method of gas mixture by using new generation NIR spectrometer is explored. To collect the NIR spectra of gas mixtures, a vacuumable gas cell was designed and assembled to Luminar 5030-731 Acousto-Optic Tunable Filter (AOTF)-NIR spectrometer. Standard gas samples of methane (CH 4), ethane (C IIH 6) and propane (C 3H 8) are diluted with super pure nitrogen via precision volumetric gas flow controllers to obtain gas mixture samples of different concentrations dynamically. The gas mixtures were injected into the gas cell and the spectra of wavelength between 1100nm-2300nm were collected. The feature components extracted from gas mixture spectra by using Partial Least Squares (PLS) were used as the inputs of the Support Vector Regress Machine (SVR) to establish the quantitative analysis model. The effectiveness of the model is tested by the samples of predicting set. The prediction Root Mean Square Error (RMSE) of CH 4, C IIH 6 and C 3H 8 is respectively 1.27%, 0.89%, and 1.20% when the concentrations of component gas are over 0.5%. It shows that the AOTF-NIR spectrometer with gas cell can be used for gas mixture analysis. PLS combining with SVR has a good performance in NIR spectroscopy analysis. This paper provides the bases for extending the application of NIR spectroscopy analysis to gas detection.

Classification of prefrontal activity due to mental arithmetic and music imagery using hidden Markov models and frequency domain near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Power, Sarah D.; Falk, Tiago H.; Chau, Tom

2010-04-01

Near-infrared spectroscopy (NIRS) has recently been investigated as a non-invasive brain-computer interface (BCI). In particular, previous research has shown that NIRS signals recorded from the motor cortex during left- and right-hand imagery can be distinguished, providing a basis for a two-choice NIRS-BCI. In this study, we investigated the feasibility of an alternative two-choice NIRS-BCI paradigm based on the classification of prefrontal activity due to two cognitive tasks, specifically mental arithmetic and music imagery. Deploying a dual-wavelength frequency domain near-infrared spectrometer, we interrogated nine sites around the frontopolar locations (International 10-20 System) while ten able-bodied adults performed mental arithmetic and music imagery within a synchronous shape-matching paradigm. With the 18 filtered AC signals, we created task- and subject-specific maximum likelihood classifiers using hidden Markov models. Mental arithmetic and music imagery were classified with an average accuracy of 77.2% ± 7.0 across participants, with all participants significantly exceeding chance accuracies. The results suggest the potential of a two-choice NIRS-BCI based on cognitive rather than motor tasks.

Uncooled infrared photon detection concepts and devices

NASA Astrophysics Data System (ADS)

Piyankarage, Viraj Vishwakantha Jayaweera

This work describes infrared (IR) photon detector techniques based on novel semiconductor device concepts and detector designs. The aim of the investigation was to examine alternative IR detection concepts with a view to resolve some of the issues of existing IR detectors such as operating temperature and response range. Systems were fabricated to demonstrate the following IR detection concepts and determine detector parameters: (i) Near-infrared (NIR) detection based on dye-sensitization of nanostructured semiconductors, (ii) Displacement currents in semiconductor quantum dots (QDs) embedded dielectric media, (iii) Split-off band transitions in GaAs/AlGaAs heterojunction interfacial workfunction internal photoemission (HEIWIP) detectors. A far-infrared detector based on GaSb homojunction interfacial workfunction internal photoemission (HIWIP) structure is also discussed. Device concepts, detector structures, and experimental results discussed in the text are summarized below. Dye-sensitized (DS) detector structures consisting of n-TiO 2/Dye/p-CuSCN heterostructures with several IR-sensitive dyes showed response peaks at 808, 812, 858, 866, 876, and 1056 nm at room temperature. The peak specific-detectivity (D*) was 9.5x1010 cm Hz-1/2 W-1 at 812 nm at room temperature. Radiation induced carrier generation alters the electronic polarizability of QDs provided the quenching of excitation is suppressed by separation of the QDs. A device constructed to illustrate this concept by embedding PbS QDs in paraffin wax showed a peak D* of 3x108 cm Hz 1/2 W-1 at ˜540 nm at ambient temperature. A typical HEIWIP/HIWIP detector structures consist of single (or multiple) period(s) of doped emitter(s) and undoped barrier(s) which are sandwiched between two highly doped contact layers. A p-GaAs/AlGaAs HEIWIP structure showed enhanced absorption in NIR range due to heavy/light-hole band to split-off band transitions and leading to the development of GaAs based uncooled sensors for IR detection in the 2--5 microm wavelength range with a peak D* of 6.8x105 cm Hz1/2 W-1. A HIWIP detector based on p-GaSb/GaSb showed a free carrier response threshold wavelength at 97 microm (˜3 THz) with a peak D* of 5.7x1011 cm Hz1/2 W-1 at 36 microm and 4.9 K. In this detector, a bolometric type response in the 97--200 microm (3--1.5 THz) range was also observed. INDEX WORDS: Infrared detectors, Photon detection, NIR detectors, THz detectors, Uncooled detectors, Dye-sensitized, IR dye, Quantum dot, Split-off band, GaSb, GaAs, AlGaAs, TiO2, CuSCN, PbS, Homojunction, Heterojunction, Workfunction, Photoemission, Displacement currents, 1/f noise.

Structure Measurements of Leaf and Woody Components of Forests with Dual-Wavelength Lidar Scanning Data

NASA Astrophysics Data System (ADS)

Strahler, A. H.; Li, Z.; Schaaf, C.; Howe, G.; Martel, J.; Hewawasam, K.; Douglas, E. S.; Chakrabarti, S.; Cook, T.; Paynter, I.; Saenz, E. J.; Wang, Z.; Woodcock, C. E.; Jupp, D. L. B.; Schaefer, M.; Newnham, G.

2014-12-01

Forest structure plays a critical role in the exchange of energy, carbon and water between land and atmosphere and nutrient cycle. We can provide detailed forest structure measurements of leaf and woody components with the Dual Wavelength Echidna® Lidar (DWEL), which acquires full-waveform scans at both near-infrared (NIR, 1064 nm) and shortwave infrared (SWIR, 1548 nm) wavelengths from simultaneous laser pulses. We collected DWEL scans at a broadleaf forest stand and a conifer forest stand at Harvard Forest in June 2014. Power returned from leaves is much lower than from woody materials such as trunks and branches at the SWIR wavelength due to the liquid water absorption by leaves, whereas returned power at the NIR wavelength is similar from both leaves and woody materials. We threshold a normalized difference index (NDI), defined as the difference between returned power at the two wavelengths divided by their sum, to classify each return pulse as a leaf or trunk/branch hit. We obtain leaf area index (LAI), woody area index (WAI) and vertical profiles of leaf and woody components directly from classified lidar hits without empirical wood-to-total ratios as are commonly used in optical methods of LAI estimation. Tree heights, diameter at breast height (DBH), and stem count density are the other forest structure parameters estimated from our DWEL scans. The separation of leaf and woody components in tandem with fine-scale forest structure measurements will benefit studies on carbon allocation of forest ecosystems and improve our understanding of the effects of forest structure on ecosystem functions. This research is supported by NSF grant, MRI-0923389

First Evidence of Vibrationally Driven Bimolecular Reactions in Solution: Reactions of Br Atoms with Dimethylsulfoxide and Methanol.

PubMed

Shin, Jae Yoon; Shaloski, Michael A; Crim, F Fleming; Case, Amanda S

2017-03-23

We present evidence for vibrational enhancement of the rate of bimolecular reactions of Br atoms with dimethylsulfoxide (DMSO) and methanol (CH 3 OH) in the condensed phase. The abstraction of a hydrogen atom from either of these solvents by a Br atom is highly endoergic: 3269 cm -1 for DMSO and 1416 or 4414 cm -1 for CH 3 OH, depending on the hydrogen atom abstracted. Thus, there is no thermal abstraction reaction at room temperature. Broadband electronic transient absorption shows that following photolysis of bromine precursors Br atoms form van der Waals complexes with the solvent molecules in about 5 ps and this Br • -solvent complex undergoes recombination. To explore the influence of vibrational energy on the abstraction reactions, we introduce a near-infrared (NIR) pump pulse following the photolysis pulse to excite the first overtone of the C-H (or O-H) stretch of the solvent molecules. Using single-wavelength detection, we observe a loss of the Br • -solvent complex that requires the presence of both photolysis and NIR pump pulses. Moreover, the magnitude of this loss depends on the NIR wavelength. Although this loss of reactive Br supports the notion of vibrationally driven chemistry, it is not concrete evidence of the hydrogen-abstraction reaction. To verify that the loss of reactive Br results from the vibrationally driven bimolecular reaction, we examine the pH dependence of the solution (as a measure of the formation of the HBr product) following long-time irradiation of the sample with both photolysis and NIR pump beams. We observe that when the NIR beam is on-resonance, the hydronium ion concentration increases fourfold as compared to that when it is off-resonance, suggesting the formation of HBr via a vibrationally driven hydrogen-abstraction reaction in solution.

Effect of V2O5 concentration on structural and optical properties of WO3 thin films prepared by RF magnetron sputtering

NASA Astrophysics Data System (ADS)

Meenakshi, M.; Gowthami, V.; Perumal, P.; Sanjeeviraja, C.

2014-10-01

Thin films of WO3 and V2O5 doped WO3 were coated on glass substrates using sputtering targets of diameter 50mm and thickness 5mm with RF power of 100 W and source to substrate distance of 60mm at room temperature for various V2O5 compositions (1, 2, 4, 6 and 10 %). XRD studies revealed that as deposited films were amorphous for all compositions. Morphological studies like Laser Raman and SEM too confirmed this amorphous nature of films. Refractive index (n) and the extinction coefficient (k) were calculated from the optical spectra such as transmittance and absorbance measured over the wavelength range of 200 to 2500nm. The films exhibited transmittance in the range of 80 to 90% in the UV-Vis-NIR region. Optical band gaps were calculated for both direct and indirect transitions. The optical parameters such as optical dispersion energies Eo and Ed, the average dielectric constant (ɛ), average values of the oscillator strength (So), wavelength of single oscillator (λo), and plasma frequency (ωp) were also calculated.

Synthesis, Characterization and Optical Constants of Silicon Oxycarbide

NASA Astrophysics Data System (ADS)

Memon, Faisal Ahmed; Morichetti, Francesco; Abro, Muhammad Ishaque; Iseni, Giosue; Somaschini, Claudio; Aftab, Umair; Melloni, Andrea

2017-03-01

High refractive index glasses are preferred in integrated photonics applications to realize higher integration scale of passive devices. With a refractive index that can be tuned between SiO2 (1.45) and a-SiC (3.2), silicon oxycarbide SiOC offers this flexibility. In the present work, silicon oxycarbide thin films from 0.1 - 2.0 μm thickness are synthesized by reactive radio frequency magnetron sputtering a silicon carbide SiC target in a controlled argon and oxygen environment. The refractive index n and material extinction coefficient k of the silicon oxycarbide films are acquired with variable angle spectroscopic ellipsometry over the UV-Vis-NIR wavelength range. Keeping argon and oxygen gases in the constant ratio, the refractive index n is found in the range from 1.41 to 1.93 at 600 nm which is almost linearly dependent on RF power of sputtering. The material extinction coefficient k has been estimated to be less than 10-4 for the deposited silicon oxycarbide films in the visible and near-infrared wavelength regions. Morphological and structural characterizations with SEM and XRD confirms the amorphous phase of the SiOC films.

Near infrared harvesting dye-sensitized solar cells enabled by rare-earth upconversion materials.

PubMed

Li, Deyang; Ågren, Hans; Chen, Guanying

2018-02-01

Dye-sensitized solar cells (DSSCs) have been deemed as promising alternatives to silicon solar cells for the conversion of clean sunlight energy into electricity. A major limitation to their conversion efficiency is their inability to utilize light in the infrared (IR) spectral range, which constitutes almost half the energy of the sun's radiation. This fact has elicited motivations and endeavors to extend the response wavelength of DSSCs to the IR range. Photon upconversion through rare-earth ions constitutes one of the most promising approaches toward the goal of converting near-IR (NIR) or IR light into visible or ultraviolet light, where DSSCs typically have high sensitivity. In the present review, we summarize recent progress based on the utilization of various upconversion materials and device structures to improve the performance of dye-sensitized solar cells.

Building-up a database of spectro-photometric standards from the UV to the NIR

NASA Astrophysics Data System (ADS)

Vernet, J.; Kerber, F.; Mainieri, V.; Rauch, T.; Saitta, F.; D'Odorico, S.; Bohlin, R.; Ivanov, V.; Lidman, C.; Mason, E.; Smette, A.; Walsh, J.; Fosbury, R.; Goldoni, P.; Groot, P.; Hammer, F.; Kaper, L.; Horrobin, M.; Kjaergaard-Rasmussen, P.; Royer, F.

2010-11-01

We present results of a project aimed at establishing a set of 12 spectro-photometric standards over a wide wavelength range from 320 to 2500 nm. Currently no such set of standard stars covering the near-IR is available. Our strategy is to extend the useful range of existing well-established optical flux standards (Oke 1990, Hamuy et al. 1992, 1994) into the near-IR by means of integral field spectroscopy with SINFONI at the VLT combined with state-of-the-art white dwarf stellar atmospheric models (TMAP, Holberg et al. 2008). As a solid reference, we use two primary HST standard white dwarfs GD71 and GD153 and one HST secondary standard BD+17 4708. The data were collected through an ESO “Observatory Programme” over ~40 nights between February 2007 and September 2008.

InGaAs detectors and FPA's for a large span of applications: design and material considerations

NASA Astrophysics Data System (ADS)

Vermeiren, J. P.; Merken, P.

2017-11-01

Compared with the other Infrared detector materials, such as HgCdTe (or MCT) and lead salts (e.g.: PbS, PbSe, PbSnTe, …), the history of InGaAs FPA's is not that old. Some 25 years ago the first linear detectors were used for space missions [1,2]. During the last 15-20 years InGaAs, grown lattice matched on InP, has become the work horse for the telecommunication industry [3] and later on for passive and active imagery in the SWIR range. For longer wavelengths than 1.7 μm, III-V materials are in strong competition with SWIR MCT and till now the performance of MCT is better than high In-content InGaAs. During the last years some alternatives based on quaternary materials [4] and on Superlattice structures [5] are making gradual progress in such a way that they can yield performing Focal planes in the (near) future. As the SWIR wavelengths range covers a large variety of applications, also the FPA characteristics and mainly the ROIC properties need to be adjusted to fulfil the mission requirements with the requested performance. Additionally one has to bear in mind that the nature of SWIR radiation is completely different from what is usually encountered in IR imaging. Whereas the signal of thermal imagery in the Middle Wavelength (MWIR: [3 - 5 μm]) or Long Wavelength (LWIR: [8 - 10 μm] or [8 - 12 μm]) band is characterized by a large DC pedestal, caused by objects at ambient temperature, and a small AC signal, due to the small temperature or emissivity variations, SWIR range imagery is characterized by a large dynamic range and almost no DC signal. In this sense the SWIR imagery is resembling more the nature of Visible and NIR imaging than that of thermal imagery.

Images from Galileo of the Venus cloud deck

USGS Publications Warehouse

Belton, M.J.S.; Gierasch, P.J.; Smith, M.D.; Helfenstein, P.; Schinder, P.J.; Pollack, James B.; Rages, K.A.; Ingersoll, A.P.; Klaasen, K.P.; Veverka, J.; Anger, C.D.; Carr, M.H.; Chapman, C.R.; Davies, M.E.; Fanale, F.P.; Greeley, R.; Greenberg, R.; Head, J. W.; Morrison, D.; Neukum, G.; Pilcher, C.B.

1991-01-01

Images of Venus taken at 418 (violet) and 986 [near-infrared (NIR)] nanometers show that the morphology and motions of large-scale features change with depth in the cloud deck. Poleward meridional velocities, seen in both spectral regions, are much reduced in the NIR. In the south polar region the markings in the two wavelength bands are strongly anticorrelated. The images follow the changing state of the upper cloud layer downwind of the subsolar point, and the zonal flow field shows a longitudinal periodicity that may be coupled to the formation of large-scale planetary waves. No optical lightning was detected.

Improving the Multi-Wavelength Capability of Chandra Large Programs

NASA Astrophysics Data System (ADS)

Pacucci, Fabio

2017-09-01

In order to fully exploit the joint Chandra/JWST/HST ventures to detect faint sources, we urgently need an advanced matching algorithm between optical/NIR and X-ray catalogs/images. This will be of paramount importance in bridging the gap between upcoming optical/NIR facilities (JWST) and later X-ray ones (Athena, Lynx). We propose to develop an advanced and automated tool to improve the identification of Chandra X-ray counterparts detected in deep optical/NIR fields based on T-PHOT, a software widely used in the community. The developed code will include more than 20 years in advancements of X-ray data analysis and will be released to the public. Finally, we will release an updated catalog of X-ray sources in the CANDELS regions: a leap forward in our endeavor of charting the Universe.

Enhanced Cellular Ablation by Attenuating Hypoxia Status and Reprogramming Tumor-Associated Macrophages via NIR Light-Responsive Upconversion Nanocrystals.

PubMed

Ai, Xiangzhao; Hu, Ming; Wang, Zhimin; Lyu, Linna; Zhang, Wenmin; Li, Juan; Yang, Huanghao; Lin, Jun; Xing, Bengang

2018-04-18

Near-infrared (NIR) light-mediated photodynamic therapy (PDT), especially based on lanthanide-doped upconversion nanocrystals (UCNs), have been extensively investigated as a promising strategy for effective cellular ablation owing to their unique optical properties to convert NIR light excitation into multiple short-wavelength emissions. Despite the deep tissue penetration of NIR light in living systems, the therapeutic efficiency is greatly restricted by insufficient oxygen supply in hypoxic tumor microenvironment. Moreover, the coexistent tumor-associated macrophages (TAMs) play critical roles in tumor recurrence during the post-PDT period. Herein, we developed a unique photosensitizer-loaded UCNs nanoconjugate (PUN) by integrating manganese dioxide (MnO 2 ) nanosheets and hyaluronic acid (HA) biopolymer to improve NIR light-mediated PDT efficacy through attenuating hypoxia status and synergistically reprogramming TAMs populations. After the reaction with overproduced H 2 O 2 in acidic tumor microenvironment, the MnO 2 nanosheets were degraded for the production of massive oxygen to greatly enhance the oxygen-dependent PDT efficiency upon 808 nm NIR light irradiation. More importantly, the bioinspired polymer HA could effectively reprogram the polarization of pro-tumor M2-type TAMs to anti-tumor M1-type macrophages to prevent tumor relapse after PDT treatment. Such promising results provided the great opportunities to achieve enhanced cellular ablation upon NIR light-mediated PDT treatment by attenuating hypoxic tumor microenvironment, and thus facilitated the rational design of new generations of nanoplatforms toward immunotherapy to inhibit tumor recurrence during post-PDT period.

Feasibility of detecting aflatoxin B1 on inoculated maize kernels surface using Vis/NIR hyperspectral imaging.

PubMed

Wang, Wei; Heitschmidt, Gerald W; Windham, William R; Feldner, Peggy; Ni, Xinzhi; Chu, Xuan

2015-01-01

The feasibility of using a visible/near-infrared hyperspectral imaging system with a wavelength range between 400 and 1000 nm to detect and differentiate different levels of aflatoxin B1 (AFB1 ) artificially titrated on maize kernel surface was examined. To reduce the color effects of maize kernels, image analysis was limited to a subset of original spectra (600 to 1000 nm). Residual staining from the AFB1 on the kernels surface was selected as regions of interest for analysis. Principal components analysis (PCA) was applied to reduce the dimensionality of hyperspectral image data, and then a stepwise factorial discriminant analysis (FDA) was performed on latent PCA variables. The results indicated that discriminant factors F2 can be used to separate control samples from all of the other groups of kernels with AFB1 inoculated, whereas the discriminant factors F1 can be used to identify maize kernels with levels of AFB1 as low as 10 ppb. An overall classification accuracy of 98% was achieved. Finally, the peaks of β coefficients of the discrimination factors F1 and F2 were analyzed and several key wavelengths identified for differentiating maize kernels with and without AFB1 , as well as those with differing levels of AFB1 inoculation. Results indicated that Vis/NIR hyperspectral imaging technology combined with the PCA-FDA was a practical method to detect and differentiate different levels of AFB1 artificially inoculated on the maize kernels surface. However, indicated the potential to detect and differentiate naturally occurring toxins in maize kernel. © 2014 Institute of Food Technologists®

Potential Benefits of Manmade Opals Demonstrated for First Time (Fact Sheet)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

NREL experiments show that disordered inverse opals significantly scatter and trap near-infrared light, with possible impact on optoelectronic materials. Inverse opals, familiar in the form of brilliantly colored opal gemstones, are a class of materials that has astounding optical properties. Scientists have been exploring the ability of inverse opals to manipulate light in the hopes of harnessing this capacity for advanced technologies such as displays, detectors, lasers, and photovoltaics. A research group at the National Renewable Energy Laboratory (NREL) discovered that man-made inverse opal films containing significant morphological disorder exhibit substantial light scattering, consequently trapping wavelengths in the near-infrared (NIR),more » which is important to a number of technologies. This discovery is the first experimental evidence to validate a 2005 theoretical model predicting the confinement of light in such structures, and it holds great promise for improving the performance of technologies that rely on careful light control. This breakthrough also makes possible optoelectronic technologies that use a range of low-cost molecular and semiconductor species that otherwise absorb light too weakly to be useful. The disordered inverse opal architecture validates the theoretical model that predicts the diffusion and confinement of light in such structures. Electrochemically deposited CdSe inverse opal films containing significant morphological disorder exhibit substantial light scattering and consequent NIR light trapping. This discovery holds promise for NIR light management in optoelectronic technologies, particularly those involving weakly absorbing molecular and semiconductor photomaterials.« less

Non-destructive technique for determining the viability of soybean (Glycine max) seeds using FT-NIR spectroscopy.

PubMed

Kusumaningrum, Dewi; Lee, Hoonsoo; Lohumi, Santosh; Mo, Changyeun; Kim, Moon S; Cho, Byoung-Kwan

2018-03-01

The viability of seeds is important for determining their quality. A high-quality seed is one that has a high capability of germination that is necessary to ensure high productivity. Hence, developing technology for the detection of seed viability is a high priority in agriculture. Fourier transform near-infrared (FT-NIR) spectroscopy is one of the most popular devices among other vibrational spectroscopies. This study aims to use FT-NIR spectroscopy to determine the viability of soybean seeds. Viable and artificial ageing seeds as non-viable soybeans were used in this research. The FT-NIR spectra of soybean seeds were collected and analysed using a partial least-squares discriminant analysis (PLS-DA) to classify viable and non-viable soybean seeds. Moreover, the variable importance in projection (VIP) method for variable selection combined with the PLS-DA was employed. The most effective wavelengths were selected by the VIP method, which selected 146 optimal variables from the full set of 1557 variables. The results demonstrated that the FT-NIR spectral analysis with the PLS-DA method that uses all variables or the selected variables showed good performance based on the high value of prediction accuracy for soybean viability with an accuracy close to 100%. Hence, FT-NIR techniques with a chemometric analysis have the potential for rapidly measuring soybean seed viability. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Near-Infrared II Fluorescence for Imaging Hindlimb Vessel Regeneration with Dynamic Tissue Perfusion Measurement

PubMed Central

Hong, Guosong; Lee, Jerry C.; Jha, Arshi; Diao, Shuo; Nakayama, Karina H.; Hou, Luqia; Doyle, Timothy C.; Robinson, Joshua T.; Antaris, Alexander L.; Dai, Hongjie; Cooke, John P.; Huang, Ngan F.

2014-01-01

Background Real-time vascular imaging that provides both anatomic and hemodynamic information could greatly facilitate the diagnosis of vascular diseases and provide accurate assessment of therapeutic effects. Here we have developed a novel fluorescence-based all-optical method, named near-infrared II (NIR-II) fluorescence imaging, to image murine hindlimb vasculature and blood flow in an experimental model of peripheral arterial disease, by exploiting fluorescence in the NIR-II region (1000–1400 nm) of photon wavelengths. Methods and Results Owing to the reduced photon scattering of NIR-II fluorescence compared to traditional NIR fluorescence imaging and thus much deeper penetration depth into the body, we demonstrated that the mouse hindlimb vasculature could be imaged with higher spatial resolution than in vivo microCT. Furthermore, imaging over 26 days revealed a significant increase in hindlimb microvascular density in response to experimentally induced ischemia within the first 8 days of the surgery (P < 0.005), which was confirmed by histological analysis of microvascular density. Moreover, the tissue perfusion in the ischemic hindlimb could be quantitatively measured by the dynamic NIR-II method, revealing the temporal kinetics of blood flow recovery that resembled microbead-based blood flowmetry and laser Doppler blood spectroscopy. Conclusions The penetration depth of millimeters, high spatial resolution and fast acquisition rate of NIR-II imaging makes it a useful imaging tool for murine models of vascular disease. PMID:24657826

Near-infrared II fluorescence for imaging hindlimb vessel regeneration with dynamic tissue perfusion measurement.

PubMed

Hong, Guosong; Lee, Jerry C; Jha, Arshi; Diao, Shuo; Nakayama, Karina H; Hou, Luqia; Doyle, Timothy C; Robinson, Joshua T; Antaris, Alexander L; Dai, Hongjie; Cooke, John P; Huang, Ngan F

2014-05-01

Real-time vascular imaging that provides both anatomic and hemodynamic information could greatly facilitate the diagnosis of vascular diseases and provide accurate assessment of therapeutic effects. Here, we have developed a novel fluorescence-based all-optical method, named near-infrared II (NIR-II) fluorescence imaging, to image murine hindlimb vasculature and blood flow in an experimental model of peripheral arterial disease, by exploiting fluorescence in the NIR-II region (1000-1400 nm) of photon wavelengths. Because of the reduced photon scattering of NIR-II fluorescence compared with traditional NIR fluorescence imaging and thus much deeper penetration depth into the body, we demonstrated that the mouse hindlimb vasculature could be imaged with higher spatial resolution than in vivo microscopic computed tomography. Furthermore, imaging during 26 days revealed a significant increase in hindlimb microvascular density in response to experimentally induced ischemia within the first 8 days of the surgery (P<0.005), which was confirmed by histological analysis of microvascular density. Moreover, the tissue perfusion in the ischemic hindlimb could be quantitatively measured by the dynamic NIR-II method, revealing the temporal kinetics of blood flow recovery that resembled microbead-based blood flowmetry and laser Doppler blood spectroscopy. The penetration depth of millimeters, high spatial resolution, and fast acquisition rate of NIR-II imaging make it a useful imaging tool for murine models of vascular disease. © 2014 American Heart Association, Inc.

A minimally invasive chip based near infrared sensor for continuous glucose monitoring

NASA Astrophysics Data System (ADS)

Ben Mohammadi, L.; Sigloch, S.; Frese, I.; Stein, V.; Welzel, K.; Schmitz, F.; Klotzbücher, T.

2012-06-01

Assessment of glycaemia in diabetes is crucially important for prevention of both, acute and long term complications. Continuous glucose monitoring (CGM) is certainly the most appropriate way for optimizing the glycaemic control, since it prevents or delays the progression of complications associated with hypo- or hyperglycaemic events, reducing morbidity, mortality, and overall costs in health care systems. In this paper we describe the concept and first in vitro results of a minimally invasive, chip-based NIR-Sensor for continuous glucose monitoring. The sensor concept is based on difference infrared absorption spectroscopy, which was evaluated within laboratory measurements of D+-Glucose dissolved in water. The laboratory measurements revealed a linear relationship between glucose concentration and the integrated difference spectroscopy signal with a coefficient of determination of 99.6% in the concentration range of 0- 500 mg/dL. Suitable wavelength bands were identified in which the correlation is preserved and commercial light sources are available for realisation of a spectrometer-less, integrated NIR-sensor. In the designed sensor the component area (non-disposable) is separated from the detection area (disposable, low-cost). The disposable part of the sensor is fluidically connected to a micro-dialyses needle, accessing glucose subcutaneously via the ISF (interstitial fluid) or intravascularly. The non-disposable part contains all the optical elements, like LED's and photo-detectors. The in- and out-coupling of the optical signal is achieved across the plane of the chip by using total internal reflection on mirrors integrated into the fluidic chip. The glucose is continuously measured by considering the difference signals of light at the corresponding wavelengths, as a function of time or in defined intervals if the light sources are modulated. The in-vitro measurements show an absolute error of about 5 mg/dL with a relative error of 5% for glucose concentrations larger than 50 mg/dL and about 12 % in the hypoglycemic range (<50 mg /dL).

Implementation of a 4x8 NIR and CCD Mosaic Focal Plane Technology

NASA Astrophysics Data System (ADS)

Jelinsky, Patrick; Bebek, C. J.; Besuner, R. W.; Haller, G. M.; Harris, S. E.; Hart, P. A.; Heetderks, H. D.; Levi, M. E.; Maldonado, S. E.; Roe, N. A.; Roodman, A. J.; Sapozhnikov, L.

2011-01-01

Mission concepts for NASA's Wide Field Infrared Survey Telescope (WFIRST), ESA's EUCLID mission, as well as for ground based observations, have requirements for large mosaic focal planes to image visible and near infrared (NIR) wavelengths. We have developed detectors, readout electronics and focal plane design techniques that can be used to create very large scalable focal plane mosaic cameras. In our technology, CCDs and HgCdTe detectors can be intermingled on a single, silicon carbide (SiC) cold plate. This enables optimized, wideband observing strategies. The CCDs, developed at Lawrence Berkeley National Laboratory, are fully-depleted, p-channel devices that are backside illuminated capable of operating at temperatures as low as 110K and have been optimized for the weak lensing dark energy technique. The NIR detectors are 1.7µm and 2.0µm wavelength cutoff H2RG® HgCdTe, manufactured by Teledyne Imaging Sensors under contract to LBL. Both the CCDs and NIR detectors are packaged on 4-side abuttable SiC pedestals with a common mounting footprint supporting a 44.16mm mosaic pitch and are coplanar. Both types of detectors have direct-attached, readout electronics that convert the detector signal directly to serial, digital data streams and allow a flexible, low cost data acquisition strategy, despite the large data volume. A mosaic of these detectors can be operated at a common temperature that achieves the required dark current and read noise performance in both types of detectors necessary for dark energy observations. We report here the design and integration for a focal plane designed to accommodate a 4x8 heterogeneous array of CCDs and HgCdTe detectors. Our current implementation contains over 1/4-billion pixels.

Near-infrared extension of a visible spectrum airborne Sun photometer

NASA Astrophysics Data System (ADS)

Starace, Marco; von Bismarck, Jonas; Hollstein, André; Ruhtz, Thomas; Preusker, René; Fischer, Jürgen

2013-05-01

The continuously-measuring, multispectral airborne Sun and aureole photometers FUBISS-ASA and FUBISSASA2 were developed at the Institute for Space Sciences of the Freie Universität Berlin in 2002 and 2006 respectively, for the retrieval of aerosol optical and microphysical parameters at wavelengths ranging from 400 to 900 nm. A multispectral near-infrared direct sun radiometer measuring in a spectral range of 1000 to 1700 nm has now been added to FUBISS-ASA2. The main objective of this NIR extension is to enhance the characterization of larger aerosol particles, as Mie scattering theory offers a more accurate approximation for their interaction with electromagnetic radiation, if both the VIS and NIR parts of the spectrum are considered, than it does for the VIS part only. The spectral transmissivity of atmospheric models was computed using the HITRAN2008 database in order to determine local absorption minima suitable for aerosol retrieval. Measurements were first carried out aboard the research vessel FS Polarstern on its transatlantic voyage ANT-XXVI/1. Additional measurements were performed from the Sphinx High Altitude Research Station on the Jungfraujoch and in the nearby Kleine Scheidegg locality during the CLACE2010 measurement campaign. Aerosol optical parameters derived from VIS aureole and direct sun measurements were compared to those of simulated aerosol mixtures in order to estimate the composition of the measured aerosol.

Obstructive sleep apnea screening by NIRS imaging

NASA Astrophysics Data System (ADS)

Kashefi, Feraydune; Watenpaugh, Donald E.; Liu, Hanli

2007-02-01

This study aimed at determining cerebral hemodynamic parameters in human subjects during breath holding using near infrared spectroscopy (NIRS). Breath holding serves as a method of simulation OSA (Obstructive Sleep Apnea). Data was acquired non-invasively from 40 subjects, twenty OSA sufferers (10 females, 10 males, age 20-70 years), and twenty normal volunteers (10 females, 10 males, age 20-65 years). Measurements were conducted using a LED Imager (LEDI) during breath holding. In comparing OSA subjects with controls during breath holding, a consistent increase or even a decrease in oxy- ([O IIHb]), deoxy- ([HHb]), total hemoglobin ([tHb]) concentrations, and tissue hemoglobin oxygen saturation (SO II) in the regional brain tissue were observed. The LEDI probe consists of 4 sources and 10 detectors serving as 4 sets of 1 source and 4 detectors each. A three wavelength (730, 805, and 850 nm) LED was used and the wavelengths were switched sequentially. The distance between sources and the source-detector separation were 2.5 cm. Data acquisition consisted of three segments, baseline for one minute, followed by a period of breath holding, and then 2 minutes of recovery time. The duration of the breath holding was subject-dependent. Our investigation proves that NIR spectroscopy could be used as a tool for detecting cerebral hemodynamics and also serves as a method of screening patients with OSA.

Lightless cataract surgery using a near-infrared operating microscope.

PubMed

Kim, Bong-Hyun

2006-10-01

To describe the near-infrared (NIR) operating microscopy (NIOM) system using the NIR wavelength as the illumination source and to evaluate the feasibility of this system for lightless cataract surgery. HenAm Kim Eye Center, Haenam-Gun, South Korea. In this noncomparative interventional case series, cataract surgery was performed in 4 patients with bilateral cataract using the NIOM system in 1 eye and conventional microscopy in the fellow eye. The primary components of the system include an optical filter, a stereoscopic camera, head-mounted displays, and a recording system. This system uses invisible NIR (wavelength 850 to 1300 nm) illumination to facilitate cataract surgery without light. The differences between the NIOM system and conventional microscopy during cataract surgery were evaluated. The NIOM system provided excellent 3-dimensional viewing in real time. The image resolution was sufficient while performing all steps of cataract surgery. Immediately postoperatively and at 10 and 30 minutes and 1 hour, the visual acuity was better in the 4 eyes in which the NIOM system was used than in the 4 eyes in which conventional microscopy was used. However, using the NIOM system required good surgical skill. Lightless cataract surgery using the NIOM system seems useful for obtaining good visual acuity immediately postoperatively. The system may also reduce the incidence of light-induced retinal toxicity and the need for mydriatic administration and be a good educational tool.

Photostimulated near-infrared persistent luminescence as a new optical read-out from Cr3+-doped LiGa5O8

PubMed Central

Liu, Feng; Yan, Wuzhao; Chuang, Yen-Jun; Zhen, Zipeng; Xie, Jin; Pan, Zhengwei

2013-01-01

In conventional photostimulable storage phosphors, the optical information written by x-ray or ultraviolet irradiation is usually read out as a visible photostimulated luminescence (PSL) signal under the stimulation of a low-energy light with appropriate wavelength. Unlike the transient PSL, here we report a new optical read-out form, photostimulated persistent luminescence (PSPL) in the near-infrared (NIR), from a Cr3+-doped LiGa5O8 NIR persistent phosphor exhibiting a super-long NIR persistent luminescence of more than 1,000 h. An intense PSPL signal peaking at 716 nm can be repeatedly obtained in a period of more than 1,000 h when an ultraviolet-light (250–360 nm) pre-irradiated LiGa5O8:Cr3+ phosphor is repeatedly stimulated with a visible light or a NIR light. The LiGa5O8:Cr3+ phosphor has promising applications in optical information storage, night-vision surveillance, and in vivo bio-imaging. PMID:23532003

NIR-light-induced surface-enhanced Raman scattering for detection and photothermal/photodynamic therapy of cancer cells using methylene blue-embedded gold nanorod@SiO2 nanocomposites

PubMed Central

Seo, Sun-Hwa; Kim, Bo-Mi; Joe, Ara; Han, Hyo-Won; Chen, Xiaoyuan; Cheng, Zhen; Jang, Eue-Soon

2015-01-01

Methylene blue-loaded gold nanorod@SiO2 (MB-GNR@SiO2) core@shell nanoparticles are synthesized for use in cancer imaging and photothermal/photodynamic dual therapy. For the preparation of GNR@SiO2 nanoparticles, we found that the silica coating rate of hexadecylcetyltrimethylammonium bromide (CTAB)-capped GNRs is much slower than that of PEGylated GNRs due to the densely coated CTAB bilayer. Encapsulated MB molecules have both monomer and dimer forms that result in an increase in the photosensitizing effect through different photochemical pathways. As a consequence of the excellent plasmonic properties of GNRs at near-infrared (NIR) light, the embedded MB molecules showed NIR light-induced SERS performance with a Raman enhancement factor of 3.0 × 1010, which is enough for the detection of a single cancer cell. Moreover, the MB-GNR@SiO2 nanoparticles exhibit a synergistic effect of photodynamic and photothermal therapies of cancer under single-wavelength NIR laser irradiation. PMID:24424205

NIR-light-induced surface-enhanced Raman scattering for detection and photothermal/photodynamic therapy of cancer cells using methylene blue-embedded gold nanorod@SiO2 nanocomposites.

PubMed

Seo, Sun-Hwa; Kim, Bo-Mi; Joe, Ara; Han, Hyo-Won; Chen, Xiaoyuan; Cheng, Zhen; Jang, Eue-Soon

2014-03-01

Methylene blue-loaded gold nanorod@SiO2 (MB-GNR@SiO2) core@shell nanoparticles are synthesized for use in cancer imaging and photothermal/photodynamic dual therapy. For the preparation of GNR@SiO2 nanoparticles, we found that the silica coating rate of hexadecylcetyltrimethylammonium bromide (CTAB)-capped GNRs is much slower than that of PEGylated GNRs due to the densely coated CTAB bilayer. Encapsulated MB molecules have both monomer and dimer forms that result in an increase in the photosensitizing effect through different photochemical pathways. As a consequence of the excellent plasmonic properties of GNRs at near-infrared (NIR) light, the embedded MB molecules showed NIR light-induced SERS performance with a Raman enhancement factor of 3.0 × 10(10), which is enough for the detection of a single cancer cell. Moreover, the MB-GNR@SiO2 nanoparticles exhibit a synergistic effect of photodynamic and photothermal therapies of cancer under single-wavelength NIR laser irradiation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Efficient extreme-UV-to-extreme-UV conversion by four-wave mixing with intense near-IR pulses in highly charged ion plasmas

NASA Astrophysics Data System (ADS)

Chu, Hsu-hsin; Wang, Jyhpyng

2018-05-01

Nonlinear optics in the extreme-ultraviolet (EUV) has been limited by lack of transparent media and small conversion efficiency. To overcome this problem we explore the advantage of using multiply charged ion plasmas as the interacting media between EUV and intense near-infrared (NIR) pulses. Such media are transparent to EUV and can withstand intense NIR driving pulses without damage. We calculate the third-order nonlinear polarizabilities of Ar2 + and Ar3 + ions for EUV and NIR four-wave mixing by using the well-proven Cowan code and find that the EUV-to-EUV conversion efficiency as high as 26% can be expected for practical experimental configurations using multi-terawatt NIR lasers. Such a high efficiency is possible because the driving pulse intensity can be scaled up to several orders of magnitude higher than in conventional nonlinear media, and the group-velocity and phase mismatch are insignificant at the experimental plasma densities. This effective scheme of wave mixing can be utilized for ultrafast EUV waveform measurement and control as well as wavelength conversion.

Indocyanine green-laser thermolysis of acne vulgaris

NASA Astrophysics Data System (ADS)

Genina, Elina A.; Bashkatov, Alexey N.; Simonenko, Georgy V.; Tuchin, Valery V.; Yaroslavsky, Ilya V.; Altshuler, Gregory B.

2005-08-01

The near-infrared (NIR) laser radiation due to its high penetration depth is widely used in phototherapy and photothermolysis. In application to skin appendages a high selectivity of laser treatment is needed to prevent light action on surrounding tissues. Indocyanine Green (ICG) dye may provide a high selectivity of treatment due to effective ICG uploading by a target and its narrow band of considerable absorption just at the wavelength of the NIR diode laser. The goal of this study is to demonstrate the efficacy of the NIR diode laser photothermolysis in combination with topical application of ICG suggested for treatment of acne vulgaris. Two volunteers with back-located acne were enrolled. Skin sites of subjects were stained by ICG and irradiated by NIR laser-diode light (803 or 809 nm). The individual acne lesions were photothermally treated at 18 W/cm2 (803 nm, 0.5 sec) without skin surface cooling or at 200 W/cm2 (809 nm, 0.5 sec) with cooling. The results of the observations during a month after the treatment have shown that ICG stained acne inflammatory elements were destructed for light exposures of 0.5 sec.

Early tumor detection afforded by in vivo imaging of near-infrared II fluorescence.

PubMed

Tao, Zhimin; Dang, Xiangnan; Huang, Xing; Muzumdar, Mandar D; Xu, Eric S; Bardhan, Neelkanth Manoj; Song, Haiqin; Qi, Ruogu; Yu, Yingjie; Li, Ting; Wei, Wei; Wyckoff, Jeffrey; Birrer, Michael J; Belcher, Angela M; Ghoroghchian, P Peter

2017-07-01

Cell-intrinsic reporters such as luciferase (LUC) and red fluorescent protein (RFP) have been commonly utilized in preclinical studies to image tumor growth and to monitor therapeutic responses. While extrinsic reporters that emit near infrared I (NIR-I: 650-950 nm) or near-infrared II (NIR-II: 1000-1700 nm) optical signals have enabled minimization of tissue autofluorescence and light scattering, it has remained unclear as to whether their use has afforded more accurate tumor imaging in small animals. Here, we developed a novel optical imaging construct comprised of rare earth lanthanide nanoparticles coated with biodegradable diblock copolymers and doped with organic fluorophores, generating NIR-I and NIR-II emissive bands upon optical excitation. Simultaneous injection of multiple spectrally-unique nanoparticles into mice bearing tumor implants established via intraperitoneal dissemination of LUC + /RFP + OVCAR-8 ovarian cancer cells enabled direct comparisons of imaging with extrinsic vs. intrinsic reporters, NIR-II vs. NIR-I signals, as well as targeted vs. untargeted exogenous contrast agents in the same animal and over time. We discovered that in vivo optical imaging at NIR-II wavelengths facilitates more accurate detection of smaller and earlier tumor deposits, offering enhanced sensitivity, improved spatial contrast, and increased depths of tissue penetration as compared to imaging with visible or NIR-I fluorescent agents. Our work further highlights the hitherto underappreciated enhancements in tumor accumulation that may be achieved with intraperitoneal as opposed to intravenous administration of nanoparticles. Lastly, we found discrepancies in the fidelity of tumor uptake that could be obtained by utilizing small molecules for in vivo as opposed to in vitro targeting of nanoparticles to disseminated tumors. Copyright © 2017 Elsevier Ltd. All rights reserved.

Near infrared spectroscopy evaluation of bladder function: the impact of skin pigmentation on detection of physiologic change during voiding

NASA Astrophysics Data System (ADS)

Shadgan, Babak; Stothers, Lynn; Molavi, Behnam; Mutabazi, Sharif; Mukisa, Ronald; Macnab, Andrew

2015-02-01

Background: Prior research indicates the epidermal pigment layer of human skin (Melanin) has a significant absorption coefficient in the near infra-red (NIR) region; hence attenuation of light in vivo is a potential confounder for NIR spectroscopy (NIRS). A NIRS method developed for transcutaneous evaluation of bladder function is being investigated as a means of improving the burden of bladder disease in sub-Saharan Africa. This required development of a simple wireless NIRS device suitable for use as a screening tool in patients with pigmented skin where the NIR light emitted would penetrate through the epidermal pigment layer and return in sufficient quantity to provide effective monitoring. Methods: Two healthy subjects, one with pigmented skin and one with fair skin, were monitored as they voided spontaneously using the prototype transcutaneous NIRS device positioned over the bladder. The device was a self-contained wireless unit with light emitting diodes (wavelengths 760 and 850 nanometres) and interoptode distance of 4cm. The raw optical data were transmitted to a laptop where graphs of chromophore change were generated with proprietary software and compared between the subjects and with prior data from asymptomatic subjects. Results: Serial monitoring was successful in both subjects. Voiding volumes varied between 350 and 380 cc. In each subject the patterns of chromophore change, trend and magnitude of change were similar and matched the physiologic increase in total and oxygenated hemoglobin recognized to occur in normal bladder contraction during voiding. Conclusions: Skin pigmentation does not compromise the ability of transcutaneous NIRS to interrogate physiologic change in the bladder during bladder contraction in healthy subjects.

Near-Infrared Spectra of Type Ia Supernovae

NASA Technical Reports Server (NTRS)

Marion, G. H.; Hoeflich, P.; Vacca, W. D.; Wheeler, J. C.

2003-01-01

We report near-infrared (NIR) spectroscopic observations of 12 'branch-normal' Type Ia supernovae (SNe Ia) that cover the wavelength region from 0.8 to 2.5 microns. Our sample more than doubles the number of SNe Ia with published NIR spectra within 3 weeks of maximum light. The epochs of observation range from 13 days before maximum light to 18 days after maximum light. A detailed model for a Type Ia supernovae is used to identify spectral features. The Doppler shifts of lines are measured to obtain the velocity and thus the radial distribution of elements. The NIR is an extremely useful tool to probe the chemical structure in the layers of SNe Ia ejecta. This wavelength region is optimal for examining certain products of the SNe Ia explosion that may be blended or obscured in other spectral regions. We identify spectral features from Mg II, Ca II, Si II, Fe II, Co II, Ni II, and possibly Mn II. We find no indications for hydrogen, helium, or carbon in the spectra. The spectral features reveal important clues about the physical characteristics of SNe Ia. We use the features to derive upper limits for the amount of unburned matter, to identify the transition regions from explosive carbon to oxygen burning and from partial to complete silicon burning, and to estimate the level of mixing during and after the explosion. Elements synthesized in the outer layers during the explosion appear to remain in distinct layers. That provides strong evidence for the presence of a detonation phase during the explosion as it occurs in delayed detonation or merger models. Mg II velocities are found to exceed 11,000 - 15,000 km/s, depending on the individual SNe Ia. That result suggests that burning during the explosion reaches the outermost layers of the progenitor and limits the amount of unburned material to less than 10% of the mass of the progenitor. Small residuals of unburned material are predicted by delayed detonation models but are inconsistent with pure deflagration or merger models. Differences in the spectra of the individual SNe Ia demonstrate the variety of these events.

Near-infrared imaging system for detecting small organic foreign substances in foods

NASA Astrophysics Data System (ADS)

Tashima, Hiroto; Genta, Tsuneaki; Ishii, Yuya; Ishiyama, Takeshi; Arai, Shinichi; Fukuda, Mitsuo

2013-09-01

Contamination of foodstuffs with foreign substances is a serious problem because it often has negative effects on consumer health. However, detection of small organic substances in foods can be difficult because they are undetectable with traditional inspection apparatus. In this work, we developed new equipment that can detect small organic contaminant substances in food at high speed using a near-infrared (NIR) imaging technique. The absorption spectra of various foods were measured, and the spectra showed low absorbance at wavelengths from 600 nm to 1150 nm. Based on the observable wavelength range of a CMOS camera, which has a high dynamic range, superluminescent diodes (SLDs) with a wavelength of 830 nm were selected as light sources. We arranged 40 SLDs on a flat panel and placed a diffusion panel over them. As a result, uniformly distributed light with an intensity of 0.26 mW/cm2 illuminated an area of 6.0 cm × 6.0 cm. Insects (3 mm wide) and hairs (0.1 mm in diameter) were embedded in stacked ham slices and in chocolate, with a total thickness of 5 mm in each case, and the transmission images were observed. Both insects and hairs were clearly observed as dark shadows with high contrast. We also compensated the images by using software developed in this study to eliminate low spatial frequency components in the images and improve the sharpness and contrast. As a result, the foreign substances were more clearly distinguished in the 5-mm-thick ham.

Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5'-dicarboxy-1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine.

PubMed

Wang, Hong; Li, Wei-Rong; Guo, Xiao-Feng; Zhang, Hua-Shan

2007-04-01

The application of near-infrared (NIR) dyes (lambda (em) > 750 nm) to the analysis of biological samples shows much promise, because the long emission wavelengths of such dyes allow interferences from biomolecule matrices to be minimized. In this paper, a novel NIR dye, 5,5'-dicarboxy-1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DCDSTCY) has been developed for the spectrophotometric determination of total protein in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportional to the concentration of protein. The linear range of the method was found to be 0.04-0.5 microg mL(-1) for bovine serum albumin (BSA) and human serum albumin (HSA), and detection limits of 5 ng mL(-1) were obtained for these substances. The maximum binding number of BSA with DCDSTCY was measured to be 133. The method proposed here has been applied to the quantitation of total protein in serum, and recoveries of 96.6-104% were achieved. Figure Near-infrared probe for protein determination.

Application of spatially modulated near-infrared structured light to study changes in optical properties of mouse brain tissue during heatstress.

PubMed

Shaul, Oren; Fanrazi-Kahana, Michal; Meitav, Omri; Pinhasi, Gad A; Abookasis, David

2017-11-10

Heat stress (HS) is a medical emergency defined by abnormally elevated body temperature that causes biochemical, physiological, and hematological changes. The goal of the present research was to detect variations in optical properties (absorption, reduced scattering, and refractive index coefficients) of mouse brain tissue during HS by using near-infrared (NIR) spatial light modulation. NIR spatial patterns with different spatial phases were used to differentiate the effects of tissue scattering from those of absorption. Decoupling optical scattering from absorption enabled the quantification of a tissue's chemical constituents (related to light absorption) and structural properties (related to light scattering). Technically, structured light patterns at low and high spatial frequencies of six wavelengths ranging between 690 and 970 nm were projected onto the mouse scalp surface while diffuse reflected light was recorded by a CCD camera positioned perpendicular to the mouse scalp. Concurrently to pattern projection, brain temperature was measured with a thermal camera positioned slightly off angle from the mouse head while core body temperature was monitored by thermocouple probe. Data analysis demonstrated variations from baseline measurements in a battery of intrinsic brain properties following HS.

Synthesis Structural and Optical Properties Of (Co, Al) co-doped ZnO Nano Particles

NASA Astrophysics Data System (ADS)

Swapna, P.; Venkatramana Reddy, S.

2018-02-01

We prepared (Co, Al) co-doped ZnO nanostructures using the method chemical co-precipitation successfully, at room temperature using PEG (Poly ethylene glycol) as stabilizing agent. Samples are prepared with different concentrations by keeping aluminium at 5 mol percent constant and varying the concentration of cobalt from 1 to 5 mol percent. After the preparation all the samples are carefully subjected to characterizations such as XRD, SEM with EDS, TEM, PL and UV-VIS-NIR. XRD pattern shows that all the samples possess hexagonal wurtzite crystal structure having no secondary phases pertaining to Al or cobalt, which shows successful dissolution of the dopents. TEM results shows the accurate size of particles and is confirmed the XRD data. SEM images of all the samples shows that particles are in nearly spherical shape, EDS spectrum reveals that incorporation of cobalt and aluminum in host lattice. PL spectrum shows that all the samples containing two prominent peaks centered at 420 nm and 446 nm. UV-VIS-NIR spectra has shown three absorptions peaks in the range of wavelength 550 nm to 700 nm, which are ascribed as typical d-d transitions of cobalt ions.

Dual fluorescent molecular substrates selectively report the activation, sustainability and reversibility of cellular PKB/Akt activity.

PubMed

Shen, Duanwen; Bai, Mingfeng; Tang, Rui; Xu, Baogang; Ju, Xiaoming; Pestell, Richard G; Achilefu, Samuel

2013-01-01

Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme's activity in real time. Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. The NIR DCF probe was highly selective for the Akt1 isoform, which was demonstrated using Akt1 knockout cells derived from MMTV-ErbB2 transgenic mice. The DCF mechanism provides unparalleled potential to assess the stimulation, sustainability, and reversibility of Akt activation longitudinally. Importantly, NIR fluorescence provides a pathway to translate findings from cells to living organisms, a condition that could eventually facilitate the use of these probes in humans.

High-Efficiency Polycrystalline Thin Film Tandem Solar Cells.

PubMed

Kranz, Lukas; Abate, Antonio; Feurer, Thomas; Fu, Fan; Avancini, Enrico; Löckinger, Johannes; Reinhard, Patrick; Zakeeruddin, Shaik M; Grätzel, Michael; Buecheler, Stephan; Tiwari, Ayodhya N

2015-07-16

A promising way to enhance the efficiency of CIGS solar cells is by combining them with perovskite solar cells in tandem devices. However, so far, such tandem devices had limited efficiency due to challenges in developing NIR-transparent perovskite top cells, which allow photons with energy below the perovskite band gap to be transmitted to the bottom cell. Here, a process for the fabrication of NIR-transparent perovskite solar cells is presented, which enables power conversion efficiencies up to 12.1% combined with an average sub-band gap transmission of 71% for photons with wavelength between 800 and 1000 nm. The combination of a NIR-transparent perovskite top cell with a CIGS bottom cell enabled a tandem device with 19.5% efficiency, which is the highest reported efficiency for a polycrystalline thin film tandem solar cell. Future developments of perovskite/CIGS tandem devices are discussed and prospects for devices with efficiency toward and above 27% are given.

Improvements of the Vis-NIRS Model in the Prediction of Soil Organic Matter Content Using Spectral Pretreatments, Sample Selection, and Wavelength Optimization

NASA Astrophysics Data System (ADS)

Lin, Z. D.; Wang, Y. B.; Wang, R. J.; Wang, L. S.; Lu, C. P.; Zhang, Z. Y.; Song, L. T.; Liu, Y.

2017-07-01

A total of 130 topsoil samples collected from Guoyang County, Anhui Province, China, were used to establish a Vis-NIR model for the prediction of organic matter content (OMC) in lime concretion black soils. Different spectral pretreatments were applied for minimizing the irrelevant and useless information of the spectra and increasing the spectra correlation with the measured values. Subsequently, the Kennard-Stone (KS) method and sample set partitioning based on joint x-y distances (SPXY) were used to select the training set. Successive projection algorithm (SPA) and genetic algorithm (GA) were then applied for wavelength optimization. Finally, the principal component regression (PCR) model was constructed, in which the optimal number of principal components was determined using the leave-one-out cross validation technique. The results show that the combination of the Savitzky-Golay (SG) filter for smoothing and multiplicative scatter correction (MSC) can eliminate the effect of noise and baseline drift; the SPXY method is preferable to KS in the sample selection; both the SPA and the GA can significantly reduce the number of wavelength variables and favorably increase the accuracy, especially GA, which greatly improved the prediction accuracy of soil OMC with Rcc, RMSEP, and RPD up to 0.9316, 0.2142, and 2.3195, respectively.

Fetal oxygenation measurement using wireless near infrared spectroscopy

NASA Astrophysics Data System (ADS)

Macnab, Andrew; Shadgan, Babak; Janssen, Patricia; Rurak, Dan

2012-03-01

Background: Fetal well-being is determined in large part by how well the placenta is able to supply oxygen and nutrients, but current technology is unable to directly measure how well a placenta functions. Near-infrared spectroscopy (NIRS) utilizes optical methods to measure tissue oxygenation. This pilot project evaluated the feasibility of NIRS for fetal monitoring through the maternal abdominal wall using a sheep model. Methods: A miniature wireless 2-wavelength NIRS device was placed on the abdominal skin over the placenta of a pregnant ewe whose fetus had been chronically catheterized to allow arterial sampling for measurement of arterial oxygen saturation. The NIRS device has 3-paired light emitting diodes and a single photodiode detector; allowing measurement of an index of tissue oxygen saturation (TSI%). Fetal limb TSI% values were compared before and during fetal breathing movements. Correlation was made during these events between arterial values and placental TSI% monitored continuously in real time. Results: Serial measurements were obtained in a single experiment. The correlation between transcutaneous NIRS derived TSI% and direct arterial oxygen saturation was very high (R2=0.86). Measures of fetal limb TSI% were declined after episodes of fetal breathing (P<0.005). Conclusions: This correlation suggests that NIRS is sensitive enough to detect changes in fetal tissue oxygenation noninvasively through the maternal abdominal wall in real-time in a sheep model. NIRS data confirmed that fetal breathing movements decrease arterial oxygen saturation in fetal lambs. If validated by further study this optical methodology could be applied as means of monitoring fetal wellbeing in humans.

In vitro near-infrared imaging of natural secondary caries.

PubMed

Simon, Jacob C; Lucas, Seth; Lee, Robert; Darling, Cynthia L; Staninec, Michal; Vanderhobli, Ram; Pelzner, Roger; Fried, Daniel

2015-02-24

Secondary caries stands as the leading reason for the failure of composite restorations and dentists spend more time replacing existing restorations than placing new ones. Current clinical strategies, and even modern visible light methods designed to detect decay, lack the sensitivity to distinguish incipient lesions, are confounded by staining on the surface and within the tooth, or are limited to detecting decay on the tooth surface. Near-IR (NIR) imaging methods, such as NIR reflectance and transillumination imaging, and optical coherence tomography are promising strategies for imaging secondary caries. Wavelengths longer than 1300-nm avoid interference from stain and exploit the greater transparency of sound enamel and dental composites, to provide increased contrast with demineralized tissues and improved imaging depth. The purpose of this study was to determine whether NIR transillumination (λ=1300-nm) and NIR cross-polarized reflectance (λ=1500-1700-nm) images can serve as reliable indicators of demineralization surrounding composite restorations. Twelve composite margins (n=12) consisting of class I, II & V restorations were chosen from ten extracted teeth. The samples were imaged in vitro using NIR transillumination and reflectance, polarization sensitive optical coherence tomography (PS-OCT) and a high-magnification digital visible light microscope. Samples were serially sectioned into 200- μ m slices for histological analysis using polarized light microscopy (PLM) and transverse microradiography (TMR). The results presented demonstrate the utility of NIR light for detecting recurrent decay and suggest that NIR images could be a reliable screening tool used in conjunction with PS-OCT for the detection and diagnosis of secondary caries.

In-vitro near-infrared imaging of natural secondary caries

NASA Astrophysics Data System (ADS)

Simon, Jacob C.; Lucas, Seth; Lee, Robert; Darling, Cynthia L.; Staninec, Michal; Vanderhobli, Ram; Pelzner, Roger; Fried, Daniel

2015-02-01

Secondary caries stands as the leading reason for the failure of composite restorations and dentists spend more time replacing existing restorations than placing new ones. Current clinical strategies, and even modern visible light methods designed to detect decay, lack the sensitivity to distinguish incipient lesions, are confounded by staining on the surface and within the tooth, or are limited to detecting decay on the tooth surface. Near-IR (NIR) imaging methods, such as NIR reflectance and transillumination imaging, and optical coherence tomography are promising strategies for imaging secondary caries. Wavelengths longer than 1300-nm avoid interference from stain and exploit the greater transparency of sound enamel and dental composites, to provide increased contrast with demineralized tissues and improved imaging depth. The purpose of this study was to determine whether NIR transillumination (λ=1300-nm) and NIR crosspolarized reflectance (λ=1500-1700-nm) images can serve as reliable indicators of demineralization surrounding composite restorations. Twelve composite margins (n=12) consisting of class I, II and V restorations were chosen from ten extracted teeth. The samples were imaged in vitro using NIR transillumination and reflectance, polarization sensitive optical coherence tomography (PS-OCT) and a high-magnification digital visible light microscope. Samples were serially sectioned into 200-μm slices for histological analysis using polarized light microscopy (PLM) and transverse microradiography (TMR). The results presented demonstrate the utility of NIR light for detecting recurrent decay and suggest that NIR images could be a reliable screening tool used in conjunction with PS-OCT for the detection and diagnosis of secondary caries.

Development of the scanning system to detect the concentration of oxy- and deoxy-hemoglobin by tracking the head

NASA Astrophysics Data System (ADS)

Ko, Woo Seok; Darwish, Naser; Gratton, Enrico; Kim, Soo Hyun

2005-04-01

We measure the concentration of oxy-, deoxy- and total hemoglobin by using the frequency-domain, near-infrared spectroscopy(NIRS) scanner. It is a non-invasive instrument that can provide real-time measurements of the changes in concentration. It can provide a diagnostic tool for the study of the brain in infants and children. However, it is difficult to apply it to the baby's head because of the contact of the probe on the soft baby's head. Therefore, we suggest the NIRS scanning system that can track the baby' head movement and detect NIRS parameters on the same position of the head. This system has three key components. The vision system performs the pattern matching for tracking the head by using the normalized cross correlation method with the target as a cross-line on the head during the diagnostic experiment. We can use the change of the position of the baby's head to re-target the light by the scanning system that uses four laser sources, a wavelength selector, and an x-y scanner. The detector system analyzes the resulting signal from the head using the diffusion model. Therefore, NIRS scanning system can provide a diagnostic tool to measure the changes of the NIRS parameters for the study of the baby's brain.

Quality assessment of pharmaceutical tablet samples using Fourier transform near infrared spectroscopy and multivariate analysis

NASA Astrophysics Data System (ADS)

Kandpal, Lalit Mohan; Tewari, Jagdish; Gopinathan, Nishanth; Stolee, Jessica; Strong, Rick; Boulas, Pierre; Cho, Byoung-Kwan

2017-09-01

Determination of the content uniformity, assessed by the amount of an active pharmaceutical ingredient (API), and hardness of pharmaceutical materials is important for achieving a high-quality formulation and to ensure the intended therapeutic effects of the end-product. In this work, Fourier transform near infrared (FT-NIR) spectroscopy was used to determine the content uniformity and hardness of a pharmaceutical mini-tablet and standard tablet samples. Tablet samples were scanned using an FT-NIR instrument and tablet spectra were collected at wavelengths of 1000-2500 nm. Furthermore, multivariate analysis was applied to extract the relationship between the FT-NIR spectra and the measured parameters. The results of FT-NIR spectroscopy for API and hardness prediction were as precise as the reference high-performance liquid chromatography and mechanical hardness tests. For the prediction of mini-tablet API content, the highest coefficient of determination for the prediction (R2p) was found to be 0.99 with a standard error of prediction (SEP) of 0.72 mg. Moreover, the standard tablet hardness measurement had a R2p value of 0.91 with an SEP of 0.25 kg. These results suggest that FT-NIR spectroscopy is an alternative and accurate nondestructive measurement tool for the detection of the chemical and physical properties of pharmaceutical samples.

On the prediction of threshold friction velocity of wind erosion using soil reflectance spectroscopy

USGS Publications Warehouse

Li, Junran; Flagg, Cody B.; Okin, Gregory S.; Painter, Thomas H.; Dintwe, Kebonye; Belnap, Jayne

2015-01-01

Current approaches to estimate threshold friction velocity (TFV) of soil particle movement, including both experimental and empirical methods, suffer from various disadvantages, and they are particularly not effective to estimate TFVs at regional to global scales. Reflectance spectroscopy has been widely used to obtain TFV-related soil properties (e.g., moisture, texture, crust, etc.), however, no studies have attempted to directly relate soil TFV to their spectral reflectance. The objective of this study was to investigate the relationship between soil TFV and soil reflectance in the visible and near infrared (VIS–NIR, 350–2500 nm) spectral region, and to identify the best range of wavelengths or combinations of wavelengths to predict TFV. Threshold friction velocity of 31 soils, along with their reflectance spectra and texture were measured in the Mojave Desert, California and Moab, Utah. A correlation analysis between TFV and soil reflectance identified a number of isolated, narrow spectral domains that largely fell into two spectral regions, the VIS area (400–700 nm) and the short-wavelength infrared (SWIR) area (1100–2500 nm). A partial least squares regression analysis (PLSR) confirmed the significant bands that were identified by correlation analysis. The PLSR further identified the strong relationship between the first-difference transformation and TFV at several narrow regions around 1400, 1900, and 2200 nm. The use of PLSR allowed us to identify a total of 17 key wavelengths in the investigated spectrum range, which may be used as the optimal spectral settings for estimating TFV in the laboratory and field, or mapping of TFV using airborne/satellite sensors.

Combined pulse-oximeter-NIRS system for biotissue diagnostics

NASA Astrophysics Data System (ADS)

Hovhannisyan, Vladimir A.

2005-08-01

Multi-wavelength (670, 805, 848 and 905 nm), multi-detector device for non-invasive measurement of biochemical components concentration in human or animal tissues, combining the methods of conventional pulse-oximetry and near infrared spectroscopy, is developed. The portable and clinically applicable system allows to measure heart pulse rate, oxygen saturation of arterial hemoglobin (pulse-oximetry method) and local absolute concentration of oxyhemoglobin, deoxyhemoglobin and oxidized cytochrome aa3 or other IR absorbed compounds (NIRS method). The system can be applied in monitoring of oxygen availability and utilization by the brain in neonatal and adults, neuro- traumatology, intensive care medicine, transplantation and plastic surgery, in sport, high-altitude and aviation medicine.

Laser-based optoelectronic system for therapy by medical treatment of cardiovascular diseases

NASA Astrophysics Data System (ADS)

Chtchoupak, Oleg S.; Shpilevoj, Boris N.; Zapaeva, Natlia L.

1996-01-01

The method and design of a system for the laser treatment of ischemic heart disease is presented. Our conceptual approach to the development of the system is based on the theoretical and experimental works of the east and west scientists about positive influence of low intensity laser irradiation in the near infrared range by treatment of cardiovascular diseases. The method and system allow active influence on the subepicardial collateral blood circulation with near infrared (NIR) laser irradiation in wavelength ranges of 0.86-1.06 mkm. The presented technique makes it possible to achieve a higher effectiveness of treatment due to individual choice of radiation parameters on the basis of analysis of the patient conditions before and after laser therapy and due to simultaneous affection at several points of the human body. Finally, results of the tests are presented, which prove given methods.

Effect of visible light on the optical properties of a-(Ge2Sb2Te5)90Ag10 thin film

NASA Astrophysics Data System (ADS)

Singh, Palwinder; Thakur, Anup

2018-05-01

(Ge2Sb2Te5)90Ag10 (GST-Ag) bulk alloy was prepared using melt quenching technique. GST-Ag thin film was deposited on glass substrate using thermal evaporation method. The prepared thin films were exposed to visible light (intensity of 105 Lux for 2, 8, 20 and 30 hours) using 25W LED lamp. Transmission spectra were taken using UV-vis-NIR spectrophotometer in the wavelength range 800-3200 nm. Optical band gap of as-deposited and light exposed thin films was determined using Tauc's plot. Optical band gap was found to be decreasing on light exposure upto 8 hours and after that no significant change was observed.

Metal-rich, Metal-poor: Updated Stellar Population Models for Old Stellar Systems

NASA Astrophysics Data System (ADS)

Conroy, Charlie; Villaume, Alexa; van Dokkum, Pieter G.; Lind, Karin

2018-02-01

We present updated stellar population models appropriate for old ages (>1 Gyr) and covering a wide range in metallicities (‑1.5 ≲ [Fe/H] ≲ 0.3). These models predict the full spectral variation associated with individual element abundance variation as a function of metallicity and age. The models span the optical–NIR wavelength range (0.37–2.4 μm), include a range of initial mass functions, and contain the flexibility to vary 18 individual elements including C, N, O, Mg, Si, Ca, Ti, and Fe. To test the fidelity of the models, we fit them to integrated light optical spectra of 41 Galactic globular clusters (GCs). The value of testing models against GCs is that their ages, metallicities, and detailed abundance patterns have been derived from the Hertzsprung–Russell diagram in combination with high-resolution spectroscopy of individual stars. We determine stellar population parameters from fits to all wavelengths simultaneously (“full spectrum fitting”), and demonstrate explicitly with mock tests that this approach produces smaller uncertainties at fixed signal-to-noise ratio than fitting a standard set of 14 line indices. Comparison of our integrated-light results to literature values reveals good agreement in metallicity, [Fe/H]. When restricting to GCs without prominent blue horizontal branch populations, we also find good agreement with literature values for ages, [Mg/Fe], [Si/Fe], and [Ti/Fe].

The Intersection of CMOS Microsystems and Upconversion Nanoparticles for Luminescence Bioimaging and Bioassays

PubMed Central

Wei, Liping.; Doughan, Samer.; Han, Yi.; DaCosta, Matthew V.; Krull, Ulrich J.; Ho, Derek.

2014-01-01

Organic fluorophores and quantum dots are ubiquitous as contrast agents for bio-imaging and as labels in bioassays to enable the detection of biological targets and processes. Upconversion nanoparticles (UCNPs) offer a different set of opportunities as labels in bioassays and for bioimaging. UCNPs are excited at near-infrared (NIR) wavelengths where biological molecules are optically transparent, and their luminesce in the visible and ultraviolet (UV) wavelength range is suitable for detection using complementary metal-oxide-semiconductor (CMOS) technology. These nanoparticles provide multiple sharp emission bands, long lifetimes, tunable emission, high photostability, and low cytotoxicity, which render them particularly useful for bio-imaging applications and multiplexed bioassays. This paper surveys several key concepts surrounding upconversion nanoparticles and the systems that detect and process the corresponding luminescence signals. The principle of photon upconversion, tuning of emission wavelengths, UCNP bioassays, and UCNP time-resolved techniques are described. Electronic readout systems for signal detection and processing suitable for UCNP luminescence using CMOS technology are discussed. This includes recent progress in miniaturized detectors, integrated spectral sensing, and high-precision time-domain circuits. Emphasis is placed on the physical attributes of UCNPs that map strongly to the technical features that CMOS devices excel in delivering, exploring the interoperability between the two technologies. PMID:25211198

Application of Hyperspectral Imaging and Chemometric Calibrations for Variety Discrimination of Maize Seeds

PubMed Central

Zhang, Xiaolei; Liu, Fei; He, Yong; Li, Xiaoli

2012-01-01

Hyperspectral imaging in the visible and near infrared (VIS-NIR) region was used to develop a novel method for discriminating different varieties of commodity maize seeds. Firstly, hyperspectral images of 330 samples of six varieties of maize seeds were acquired using a hyperspectral imaging system in the 380–1,030 nm wavelength range. Secondly, principal component analysis (PCA) and kernel principal component analysis (KPCA) were used to explore the internal structure of the spectral data. Thirdly, three optimal wavelengths (523, 579 and 863 nm) were selected by implementing PCA directly on each image. Then four textural variables including contrast, homogeneity, energy and correlation were extracted from gray level co-occurrence matrix (GLCM) of each monochromatic image based on the optimal wavelengths. Finally, several models for maize seeds identification were established by least squares-support vector machine (LS-SVM) and back propagation neural network (BPNN) using four different combinations of principal components (PCs), kernel principal components (KPCs) and textural features as input variables, respectively. The recognition accuracy achieved in the PCA-GLCM-LS-SVM model (98.89%) was the most satisfactory one. We conclude that hyperspectral imaging combined with texture analysis can be implemented for fast classification of different varieties of maize seeds. PMID:23235456

High precision radial velocities with GIANO spectra

NASA Astrophysics Data System (ADS)

Carleo, I.; Sanna, N.; Gratton, R.; Benatti, S.; Bonavita, M.; Oliva, E.; Origlia, L.; Desidera, S.; Claudi, R.; Sissa, E.

2016-06-01

Radial velocities (RV) measured from near-infrared (NIR) spectra are a potentially excellent tool to search for extrasolar planets around cool or active stars. High resolution infrared (IR) spectrographs now available are reaching the high precision of visible instruments, with a constant improvement over time. GIANO is an infrared echelle spectrograph at the Telescopio Nazionale Galileo (TNG) and it is a powerful tool to provide high resolution spectra for accurate RV measurements of exoplanets and for chemical and dynamical studies of stellar or extragalactic objects. No other high spectral resolution IR instrument has GIANO's capability to cover the entire NIR wavelength range (0.95-2.45 μm) in a single exposure. In this paper we describe the ensemble of procedures that we have developed to measure high precision RVs on GIANO spectra acquired during the Science Verification (SV) run, using the telluric lines as wavelength reference. We used the Cross Correlation Function (CCF) method to determine the velocity for both the star and the telluric lines. For this purpose, we constructed two suitable digital masks that include about 2000 stellar lines, and a similar number of telluric lines. The method is applied to various targets with different spectral type, from K2V to M8 stars. We reached different precisions mainly depending on the H-magnitudes: for H ˜ 5 we obtain an rms scatter of ˜ 10 m s-1, while for H ˜ 9 the standard deviation increases to ˜ 50 ÷ 80 m s-1. The corresponding theoretical error expectations are ˜ 4 m s-1 and 30 m s-1, respectively. Finally we provide the RVs measured with our procedure for the targets observed during GIANO Science Verification.

[Determination of Chloride Salt Solution by NIR Spectroscopy].

PubMed

Zhang, Bin; Chen, Jian-hong; Jiao, Ming-xing

2015-07-01

Determination of chloride salt solution by near infrared spectrum plays a very important role in Biomedicine. The near infrared spectrum analysis of Sodium chloride, potassium chloride, calcium chloride aqueous solution shows that the concentration change of chloride salt can affect hydrogen bond, resulting in the variation of near infrared spectrum of water. The temperature influence on NIR spectrum has been decreased by choosing reasonable wavelength range and the wavelength where the temperature effects are zero (isosbestic point). Chlorine salt prediction model was established based on partial least squares method and used for predicting the concentration of the chlorine ion. The impact on near infrared spectrum of the cation ionic radius, the number of ionic charge, the complex effect of ionic in water has also discussed in this article and the reason of every factor are analysed. Experimental results show that the temperature and concentration will affect the near-infrared spectrum of the solution, It is found that the effect of temperature plays the dominant role at low concentrations of chlorine salt; rather, the ionic dominates at high concentration. Chloride complexes are formed in aqueous solution, It has an effect on hydrogen bond of water combining with the cations in chlorine salt solution, Comparing different chloride solutions at the same concentration, the destruction effects of chloride complexes and catnions on the hydrogen bond of water increases in the sequences: CaCl2 >NaCl>KC. The modeling result shows that the determination coefficients (R2) = 99.97%, the root mean square error of cross validation (RM- SECV) = 4.51, and the residual prediction deviation (RPD) = 62.7, it meets the daily requirements of biochemical detection accuracy.

Determination of geographical origin of alcoholic beverages using ultraviolet, visible and infrared spectroscopy: A review

NASA Astrophysics Data System (ADS)

Uríčková, Veronika; Sádecká, Jana

2015-09-01

The identification of the geographical origin of beverages is one of the most important issues in food chemistry. Spectroscopic methods provide a relative rapid and low cost alternative to traditional chemical composition or sensory analyses. This paper reviews the current state of development of ultraviolet (UV), visible (Vis), near infrared (NIR) and mid infrared (MIR) spectroscopic techniques combined with pattern recognition methods for determining geographical origin of both wines and distilled drinks. UV, Vis, and NIR spectra contain broad band(s) with weak spectral features limiting their discrimination ability. Despite this expected shortcoming, each of the three spectroscopic ranges (NIR, Vis/NIR and UV/Vis/NIR) provides average correct classification higher than 82%. Although average correct classification is similar for NIR and MIR regions, in some instances MIR data processing improves prediction. Advantage of using MIR is that MIR peaks are better defined and more easily assigned than NIR bands. In general, success in a classification depends on both spectral range and pattern recognition methods. The main problem still remains the construction of databanks needed for all of these methods.

Green synthesis of anisotropic gold nanoparticles for photothermal therapy of cancer.

PubMed

Fazal, Sajid; Jayasree, Aswathy; Sasidharan, Sisini; Koyakutty, Manzoor; Nair, Shantikumar V; Menon, Deepthy

2014-06-11

Nanoparticles of varying composition, size, shape, and architecture have been explored for use as photothermal agents in the field of cancer nanomedicine. Among them, gold nanoparticles provide a simple platform for thermal ablation owing to its biocompatibility in vivo. However, the synthesis of such gold nanoparticles exhibiting suitable properties for photothermal activity involves cumbersome routes using toxic chemicals as capping agents, which can cause concerns in vivo. Herein, gold nanoparticles, synthesized using green chemistry routes possessing near-infrared (NIR) absorbance facilitating photothermal therapy, would be a viable alternative. In this study, anisotropic gold nanoparticles were synthesized using an aqueous route with cocoa extract which served both as a reducing and stabilizing agent. The as-prepared gold nanoparticles were subjected to density gradient centrifugation to maximize its NIR absorption in the wavelength range of 800-1000 nm. The particles also showed good biocompatibility when tested in vitro using A431, MDA-MB231, L929, and NIH-3T3 cell lines up to concentrations of 200 μg/mL. Cell death induced in epidermoid carcinoma A431 cells upon irradiation with a femtosecond laser at 800 nm at a low power density of 6 W/cm(2) proved the suitability of green synthesized NIR absorbing anisotropic gold nanoparticles for photothermal ablation of cancer cells. These gold nanoparticles also showed good X-ray contrast when tested using computed tomography (CT), proving their feasibility for use as a contrast agent as well. This is the first report on green synthesized anisotropic and cytocompatible gold nanoparticles without any capping agents and their suitability for photothermal therapy.

On-line milk spectrometry: analysis of bovine milk composition

NASA Astrophysics Data System (ADS)

Spitzer, Kyle; Kuennemeyer, Rainer; Woolford, Murray; Claycomb, Rod

2005-04-01

We present partial least squares (PLS) regressions to predict the composition of raw, unhomogenised milk using visible to near infrared spectroscopy. A total of 370 milk samples from individual quarters were collected and analysed on-line by two low cost spectrometers in the wavelength ranges 380-1100 nm and 900-1700 nm. Samples were collected from 22 Friesian, 17 Jersey, 2 Ayrshire and 3 Friesian-Jersey crossbred cows over a period of 7 consecutive days. Transmission spectra were recorded in an inline flowcell through a 0.5 mm thick milk sample. PLS models, where wavelength selection was performed using iterative PLS, were developed for fat, protein, lactose, and somatic cell content. The root mean square error of prediction (and correlation coefficient) for the nir and visible spectrometers respectively were 0.70%(0.93) and 0.91%(0.91) for fat, 0.65%(0.5) and 0.47%(0.79) for protein, 0.36%(0.49) and 0.45%(0.43) for lactose, and 0.50(0.54) and 0.48(0.51) for log10 somatic cells.

Multispectral Near-Infrared Imaging of Composite Restorations in Extracted Teeth.

PubMed

Logan, Cooper M; Co, Katrina U; Fried, William A; Simon, Jacob C; Staninec, Michal; And, Daniel Fried; Darling, Cynthia L

2014-02-20

One major advantage of composite restoration materials is that they can be color matched to the tooth. However, this presents a challenge when composites fail and they need to be replaced. Dentists typically spend more time repairing and replacing composites than placing new restorations. Previous studies have shown that near-infrared imaging can be used to distinguish between sound enamel and decay due to the differences in light scattering. The purpose of this study was to use a similar approach and exploit differences in light scattering to attain high contrast between composite and tooth structure. Extracted human teeth with composites (n=16) were imaged in occlusal transmission mode at wavelengths of 1300-nm, 1460-nm and 1550-nm using an InGaAs image sensor with a tungsten halogen light source with spectral filters. All samples were also imaged in the visible range using a high definition 3D digital microscope. Our results indicate that NIR wavelengths at 1460-nm and 1550-nm, coincident with higher water absorption yield the highest contrast between dental composites and tooth structure.

Spectrophotometric evaluation of optical performances of polarizing technologies for smart window applications

NASA Astrophysics Data System (ADS)

Levati, N.; Vitali, L.; Fustinoni, D.; Niro, A.

2014-11-01

In recent years, window-integrated solar protection systems are used and studied as a promising energy saving technology, both for cold and hot climates. In particular, smart windows, whose optical proprieties in the solar wavelength range can somehow be controlled, show interesting results, especially in reducing the air conditioning power consumption. With the improvement of nanolithography techniques as well as with the possibility of designing polarization intervals, coupled polarizing films show a good potential as a dynamic and wavelength-selective shading technology. In this paper, UV-Vis-NIR spectrophotometric measurements are carried out on two polarizing technologies, Polaroid crystalline polarizer and Wire Grid broadband polarizer, in single- and double- film layout, to evaluate their optical performances, i.e. spectral transmittance, reflectance and absorptivity. The solar radiation glazing factors, according to the standard UNI EN 410, are calculated. The measured data are also analyzed in detail to emphasize the optical peculiarities of the materials under study that do not stand out from the standard parameters, as well as the specific problems that arise in spectrophotometric evaluations of polarizing films.

Multispectral near-infrared imaging of composite restorations in extracted teeth

NASA Astrophysics Data System (ADS)

Logan, Cooper M.; Co, Katrina U.; Fried, William A.; Simon, Jacob C.; Staninec, Michal; Fried, Daniel; Darling, Cynthia L.

2014-02-01

One major advantage of composite restoration materials is that they can be color matched to the tooth. However, this presents a challenge when composites fail and they need to be replaced. Dentists typically spend more time repairing and replacing composites than placing new restorations. Previous studies have shown that near-infrared imaging can be used to distinguish between sound enamel and decay due to the differences in light scattering. The purpose of this study was to use a similar approach and exploit differences in light scattering to attain high contrast between composite and tooth structure. Extracted human teeth with composites (n=16) were imaged in occlusal transmission mode at wavelengths of 1300-nm, 1460-nm and 1550-nm using an InGaAs image sensor with a tungsten halogen light source with spectral filters. All samples were also imaged in the visible range using a high definition 3D digital microscope. Our results indicate that NIR wavelengths at 1460-nm and 1550-nm, coincident with higher water absorption yield the highest contrast between dental composites and tooth structure.

Measurement of dust optical depth using the solar irradiance sensor (SIS) onboard the ExoMars 2016 EDM

NASA Astrophysics Data System (ADS)

Toledo, D.; Arruego, I.; Apéstigue, V.; Jiménez, J. J.; Gómez, L.; Yela, M.; Rannou, P.; Pommereau, J.-P.

2017-04-01

The solar irradiance sensor (SIS) was included in the DREAMS package onboard the ExoMars 2016 Entry Descent and Landing Demonstrator Module, and has been selected in the METEO meteorological station onboard the ExoMars 2020 Lander. This instrument is designed to measure at different time intervals the scattered flux or the sum of direct flux and scattered flux in UVA (315-400 nm) and NIR (700-1100 nm) bands. For SIS'16, these measurements are performed by a total of 3 sensors per band placed at the faces of a truncated tetrahedron with face inclination angles of 60°. The principal goal of SIS'16 design is to perform measurements of the dust opacity in UVA and NIR wavelengths ranges, crucial parameters in the understanding of the Martian dust cycle. The retrieval procedure is based on the use of radiative transfer simulations to reproduce SIS observations acquired during daytime as a function of dust opacity. Based on different sensitivity analysis, the retrieval procedure also requires to include as free parameters (1) the dust effective radius; (2) the dust effective variance; and (3) the imaginary part of the refractive index of dust particles in UVA band. We found that the imaginary part of the refractive index of dust particles does not have a big impact on NIR signal, and hence we can kept constant this parameter in the retrieval of dust opacity at this channel. In addition to dust opacity measurements, this instrument is also capable to detect and characterize clouds by looking at the time variation of the color index (CI), defined as the ratio between the observations in NIR and UVA channels, during daytime or twilight. By simulating CI signals with a radiative transfer model, the cloud opacity and cloud altitude (only during twilight) can be retrieved. Here the different retrieval procedures that are used to analyze SIS measurements, as well as the results obtained in different sensitivity analysis, are presented and discussed.

Rapid assessment of bioactive phenolics and methylxanthines in spent coffee grounds by FT-NIR spectroscopy.

PubMed

Magalhães, Luís M; Machado, Sandia; Segundo, Marcela A; Lopes, João A; Páscoa, Ricardo N M J

2016-01-15

Spent coffee grounds (SCGs) are a great source of bioactive compounds with interest to pharmaceutical and cosmetic industries. Phenolics and methylxanthines are the main health related compounds present in SCG samples. Content estimation of these compounds in SCGs is of upmost importance in what concerns their profitable use by waste recovery industries. In the present work, near-infrared spectroscopy (NIRS) was proposed as a rapid and non-destructive technique to assess the content of three main phenolics (caffeic acid, (+)-catechin and chlorogenic acid) and three methylxanthines (caffeine, theobromine and theophylline) in SCG samples obtained from different coffee brands and diverse coffee machines. The content of these compounds was determined for 61 SCG samples by HPLC coupled with diode-array detection. Partial least squares (PLS) regression based models were calibrated to correlate diffuse reflectance NIR spectra against the reference data for the six parameters obtained by HPLC. Spectral wavelength selection and number of latent variables were optimized by minimizing the cross-validation error. PLS models showed good linearity with a coefficient of determination for the prediction set (Rp(2)) of 0.95, 0.92, 0.88, 071 and 0.84 for caffeine, caffeic acid, (+)-catechin, chlorogenic acid and theophylline, respectively. The range error ratio (RER) was higher for caffeine (17.8) when compared to other compounds (12.0, 10.1, 7.6 and 9.2, respectively for caffeic acid, (+)-catechin, chlorogenic acid and theophylline). Moreover, the content of caffeine could be used to predict the antioxidant properties of SCG samples (R=0.808, n=61), despite not presenting this property itself. The results obtained confirmed that NIRS is a suitable technique to screen SCG samples unveiling those with high content of bioactive compounds, which are interesting for subsequent extraction procedures. Copyright © 2015 Elsevier B.V. All rights reserved.

Nondestructive detection of zebra chip disease in potatoes using near-infrared spectroscopy

USDA-ARS?s Scientific Manuscript database

Near-Infrared (NIR) spectroscopy in the wavelength region from 900 nm to 2600 nm was evaluated as the basis for a rapid, non-destructive method for the detection of Zebra Chip disease in potatoes and the measurement of sugar concentrations in affected tubers. Using stepwise regression in conjunction...

Detection of pit fragments in fresh cherries using near infrared spectroscopy

USDA-ARS?s Scientific Manuscript database

NIR spectroscopy in the wavelength region from 900nm to 2600nm was evaluated as the basis for a rapid, non-destructive method for the detection of pits and pit fragments in fresh cherries. Partial Least Squares discriminant analysis (PLS-DA) following various spectral pretreatments was applied to sp...

Magnetic resonance guided optical spectroscopy imaging of human breast cancer using a combined frequency domain and continuous wave approach

NASA Astrophysics Data System (ADS)

Mastanduno, Michael A.; Davis, Scott C.; Jiang, Shudong; diFlorio-Alexander, Roberta; Pogue, Brian W.; Paulsen, Keith D.

2012-03-01

Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) is used to image high-risk patients for breast cancer because of its higher sensitivity to tumors (approaching 100%) than traditional x-ray mammography. We focus on Near Infrared Spectroscopy (NIRS) as an emerging functional and molecular imaging technique that non-invasively quantifies optical properties of total hemoglobin, oxygen saturation, water content, scattering, and lipid concentration to increase the relatively low specificity of DCE-MRI. Our optical imaging system combines six frequency domain wavelengths, measured using PMT detectors with three continuous wave wavelengths measured using CCD/spectrometers. We present methods on combining the synergistic attributes of DCE-MR and NIRS for in-vivo imaging of breast cancer in three dimensions using a custom optical MR breast coil and diffusion based light modeling software, NIRFAST. We present results from phantom studies, healthy subjects, and breast cancer patients. Preliminary results show contrast recovery within 10% in phantoms and spatial resolution less than 5mm. Images from healthy subjects were recovered with properties similar to literature values and previous studies. Patient images have shown elevated total hemoglobin values and water fraction, agreeing with histology and previous results. The additional information gained from NIRS may improve the ability to distinguish between malignant and benign lesions during MR imaging. These dual modality instruments will provide complex anatomical and molecular prognostic information, and may decrease the number of biopsies, thereby improving patient care.

Replacing effective spectral radiance by temperature in occupational exposure limits to protect against retinal thermal injury from light and near IR radiation.

PubMed

Madjidi, Faramarz; Behroozy, Ali

2014-01-01

Exposure to visible light and near infrared (NIR) radiation in the wavelength region of 380 to 1400 nm may cause thermal retinal injury. In this analysis, the effective spectral radiance of a hot source is replaced by its temperature in the exposure limit values in the region of 380-1400 nm. This article describes the development and implementation of a computer code to predict those temperatures, corresponding to the exposure limits proposed by the American Conference of Governmental Industrial Hygienists (ACGIH). Viewing duration and apparent diameter of the source were inputs for the computer code. At the first stage, an infinite series was created for calculation of spectral radiance by integration with Planck's law. At the second stage for calculation of effective spectral radiance, the initial terms of this infinite series were selected and integration was performed by multiplying these terms by a weighting factor R(λ) in the wavelength region 380-1400 nm. At the third stage, using a computer code, the source temperature that can emit the same effective spectral radiance was found. As a result, based only on measuring the source temperature and accounting for the exposure time and the apparent diameter of the source, it is possible to decide whether the exposure to visible and NIR in any 8-hr workday is permissible. The substitution of source temperature for effective spectral radiance provides a convenient way to evaluate exposure to visible light and NIR.

Implanted near-infrared spectroscopy for cardiac monitoring

NASA Astrophysics Data System (ADS)

Bhunia, Sourav K.; Cinbis, Can

2011-02-01

Implanted Cardioverter Defibrillator (ICD) provides one of the most effective therapies for the prevention of sudden cardiac death, but also delivers some high voltage shocks inappropriately, causing morbidity and mortality. Implanted near-infrared spectroscopy (NIRS) may augment ICD arrhythmia detection by monitoring skeletal muscle perfusion. A two-wavelength, single-distance, continuous-wave implanted NIRS has been evaluated in-vivo. A weighted difference of the changes in attenuation at two wavelengths, across the isobestic point of the hemoglobin spectra, was taken to be the microvascular oxygenation trend indicator (O2 Index). Although the exact weight depends on the local vascular distribution and their oxygen levels, the hypothesis that a constant weight may be adequate for hemodynamic trending during short arrhythmic episodes, was tested. The sensor was implanted subcutaneously both on fresh tissue and inside scar tissue that formed around a pre-existing implant, in 3 animals each. Attenuations were recorded at 660 and 890 nm during normal sinus rhythm (NSR) and induced ventricular fibrillation (VF). The slope of the O2 Index over 10 seconds was computed for 7 NSR and 8 VF episodes in fresh and 13 NSR and 15 VF episodes in scar tissue pockets. The mean O2 Index slope was significantly different (p<0.0001) between NSR and VF rhythms for both the fresh and scar tissue pockets. Therefore implanted NIRS may be useful for preventing inappropriate detection of VF during electromagnetic interference, double counting of ECG T-wave as an R-wave, ICD lead failure, electrocardiographic aberrancy etc.

Design of liver functional reserve monitor based on three-wavelength from IR to NIR.

PubMed

Ye, Fuli; Zhan, Huimiao; Shi, Guilian

2018-05-04

The preoperative evaluation of liver functional reserve is very important to determine the excision of liver lobe for the patients with liver cancer. There already exist many effective evaluation methods, but these ones have many disadvantages such as large trauma, complicated process and so on. Therefore, it is essential to develop a fast, accurate and simple detection method of liver functional reserve for the practical application in the clinical engineering field. According to the principle of spectrophotometry, this paper proposes a detection method of liver functional reserve based on three-wavelength from infrared light (IR) to near-infrared light (NIR), in which the artery pulse, the vein pulse and the move of tissue are taken into account. By using near-infrared photoelectric sensor technology and excreting experiment of indocyanine green, a minimally invasive, fast and simple testing equipment is designed in this paper. The testing result shows this equipment can greatly reduce the interference from human body and ambient, realize continuous and real-time detection of arterial degree of blood oxygen saturation and liver functional reserve.

Factors affecting the accuracy of near-infrared spectroscopy concentration calculations for focal changes in oxygenation parameters

NASA Technical Reports Server (NTRS)

Strangman, Gary; Franceschini, Maria Angela; Boas, David A.; Sutton, J. P. (Principal Investigator)

2003-01-01

Near-infrared spectroscopy (NIRS) can be used to noninvasively measure changes in the concentrations of oxy- and deoxyhemoglobin in tissue. We have previously shown that while global changes can be reliably measured, focal changes can produce erroneous estimates of concentration changes (NeuroImage 13 (2001), 76). Here, we describe four separate sources for systematic error in the calculation of focal hemoglobin changes from NIRS data and use experimental methods and Monte Carlo simulations to examine the importance and mitigation methods of each. The sources of error are: (1). the absolute magnitudes and relative differences in pathlength factors as a function of wavelength, (2). the location and spatial extent of the absorption change with respect to the optical probe, (3). possible differences in the spatial distribution of hemoglobin species, and (4). the potential for simultaneous monitoring of multiple regions of activation. We found wavelength selection and optode placement to be important variables in minimizing such errors, and our findings indicate that appropriate experimental procedures could reduce each of these errors to a small fraction (<10%) of the observed concentration changes.

Long wavelength fluorescence based biosensors for in vivo continuous monitoring of metabolites

NASA Astrophysics Data System (ADS)

Thomas, Joseph; Ambroise, Arounaguiry; Birchfield, Kara; Cai, Wensheng; Sandmann, Christian; Singh, Sarabjit; Weidemaier, Kristin; Pitner, J. Bruce

2006-02-01

The early stage development studies of novel implantable continuous metabolite sensor systems for glucose, lactate and fatty acids are discussed. These sensors utilize non-enzymatic "reagentless" sensor systems based on NIR fluorophore-labeled binding proteins. For in vivo applications, NIR fluorescence based systems (beyond 600 nm) have the added benefit of reduced interference from background scattering, tissue and serum absorption and cell auto-fluorescence. The long wavelength emission facilitates implanted sensor disks to transmit fluorescence to an external reader through wireless connections and the resulting fluorescence signals can be correlated to metabolite concentrations. We have developed a prototype optical system that uses a bifurcated optical fiber to transmit excitation and read emission at the surface of the skin. With this system, fluorescence signals were read over time through animal skin. The changes in glucose concentration were studied using immobilized sensor proteins and were compared to non-immobilized sensors in solution. For sensors in solution, no response delay was observed. For immobilized systems, the fluorescence response showed a delay corresponding to the diffusion time for the metabolite to equilibrate within the sensor.

Identification of pesticide varieties by detecting characteristics of Chlorella pyrenoidosa using Visible/Near infrared hyperspectral imaging and Raman microspectroscopy technology.

PubMed

Shao, Yongni; Li, Yuan; Jiang, Linjun; Pan, Jian; He, Yong; Dou, Xiaoming

2016-11-01

The main goal of this research is to examine the feasibility of applying Visible/Near-infrared hyperspectral imaging (Vis/NIR-HSI) and Raman microspectroscopy technology for non-destructive identification of pesticide varieties (glyphosate and butachlor). Both mentioned technologies were explored to investigate how internal elements or characteristics of Chlorella pyrenoidosa change when pesticides are applied, and in the meantime, to identify varieties of the pesticides during this procedure. Successive projections algorithm (SPA) was introduced to our study to identify seven most effective wavelengths. With those wavelengths suggested by SPA, a model of the linear discriminant analysis (LDA) was established to classify the pesticide varieties, and the correct classification rate of the SPA-LDA model reached as high as 100%. For the Raman technique, a few partial least squares discriminant analysis models were established with different preprocessing methods from which we also identified one processing approach that achieved the most optimal result. The sensitive wavelengths (SWs) which are related to algae's pigment were chosen, and a model of LDA was established with the correct identification reached a high level of 90.0%. The results showed that both Vis/NIR-HSI and Raman microspectroscopy techniques are capable to identify pesticide varieties in an indirect but effective way, and SPA is an effective wavelength extracting method. The SWs corresponding to microalgae pigments, which were influenced by pesticides, could also help to characterize different pesticide varieties and benefit the variety identification. Copyright © 2016 Elsevier Ltd. All rights reserved.

Dynamic topographical pattern classification of multichannel prefrontal NIRS signals: II. Online differentiation of mental arithmetic and rest

NASA Astrophysics Data System (ADS)

Schudlo, Larissa C.; Chau, Tom

2014-02-01

Objective. Near-infrared spectroscopy (NIRS) has recently gained attention as a modality for brain-computer interfaces (BCIs), which may serve as an alternative access pathway for individuals with severe motor impairments. For NIRS-BCIs to be used as a real communication pathway, reliable online operation must be achieved. Yet, only a limited number of studies have been conducted online to date. These few studies were carried out under a synchronous paradigm and did not accommodate an unconstrained resting state, precluding their practical clinical implication. Furthermore, the potentially discriminative power of spatiotemporal characteristics of activation has yet to be considered in an online NIRS system. Approach. In this study, we developed and evaluated an online system-paced NIRS-BCI which was driven by a mental arithmetic activation task and accommodated an unconstrained rest state. With a dual-wavelength, frequency domain near-infrared spectrometer, measurements were acquired over nine sites of the prefrontal cortex, while ten able-bodied participants selected letters from an on-screen scanning keyboard via intentionally controlled brain activity (using mental arithmetic). Participants were provided dynamic NIR topograms as continuous visual feedback of their brain activity as well as binary feedback of the BCI's decision (i.e. if the letter was selected or not). To classify the hemodynamic activity, temporal features extracted from the NIRS signals and spatiotemporal features extracted from the dynamic NIR topograms were used in a majority vote combination of multiple linear classifiers. Main results. An overall online classification accuracy of 77.4 ± 10.5% was achieved across all participants. The binary feedback was found to be very useful during BCI use, while not all participants found value in the continuous feedback provided. Significance. These results demonstrate that mental arithmetic is a potent mental task for driving an online system-paced NIRS-BCI. BCI feedback that reflects the classifier's decision has the potential to improve user performance. The proposed system can provide a framework for future online NIRS-BCI development and testing.

High-throughput quantitative biochemical characterization of algal biomass by NIR spectroscopy; multiple linear regression and multivariate linear regression analysis.

PubMed

Laurens, L M L; Wolfrum, E J

2013-12-18

One of the challenges associated with microalgal biomass characterization and the comparison of microalgal strains and conversion processes is the rapid determination of the composition of algae. We have developed and applied a high-throughput screening technology based on near-infrared (NIR) spectroscopy for the rapid and accurate determination of algal biomass composition. We show that NIR spectroscopy can accurately predict the full composition using multivariate linear regression analysis of varying lipid, protein, and carbohydrate content of algal biomass samples from three strains. We also demonstrate a high quality of predictions of an independent validation set. A high-throughput 96-well configuration for spectroscopy gives equally good prediction relative to a ring-cup configuration, and thus, spectra can be obtained from as little as 10-20 mg of material. We found that lipids exhibit a dominant, distinct, and unique fingerprint in the NIR spectrum that allows for the use of single and multiple linear regression of respective wavelengths for the prediction of the biomass lipid content. This is not the case for carbohydrate and protein content, and thus, the use of multivariate statistical modeling approaches remains necessary.

A novel near-infrared nanomaterial with high quantum efficiency and its applications in real time in vivo imaging

NASA Astrophysics Data System (ADS)

Cui, X. X.; Fan, Q.; Shi, S. J.; Wen, W. H.; Chen, D. F.; Guo, H. T.; Xu, Y. T.; Gao, F.; Nie, R. Z.; Ford, Harold D.; Tang, Gordon H.; Hou, C. Q.; Peng, B.

2018-05-01

Fluorescence imaging signal is severely limited by the quantum efficiency and emission wavelength. To overcome these challenges, novel NIR-emitting K5NdLi2F10 nanoparticles under NIR excitation was introduced as fluorescence imaging probe for the first time. The photostability of K5NdLi2F10 nanoparticles in the water, phosphate buffer saline, fetal bovine serum and living mice was investigated. The fluorescence signal was detected with depths of 3.5 and 2.0 cm in phantom and pork tissue, respectively. Fluorescence spectrum with a significant signal-to-background ratio of 10:1 was captured in living mice. Moreover, clear NIR images were virtualized for the living mice after intravenous injection. The imaging ability of nanoparticles in tumor-beard mice were evaluated, the enrichment of K5NdLi2F10 nanoparticles in tumor site due to the enhanced permeability and retention effect was confirmed. The systematic studies of toxicity, bio-distribution and in-vivo dynamic imaging suggest that these materials give high biocompatibility and low toxicity. These NIR-emitting nanoparticles with high quantum efficiency, high penetration and low toxicity might facilitate tumor identification in deep tissues more sensitively.

NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers

NASA Astrophysics Data System (ADS)

Gellerich, Frank N.; Mueller, Tobias; Nioka, Shoko; Hertel, Katrin; Schulte-Mattler, Wilhelm J.; Zierz, Stephan; Chance, Britton

1998-01-01

Noninvasive measurement of changes in oxygenation of human skeletal muscle can be done with a dual-wavelength near infrared (NIR) spectrophotometer. This allows a noninvasive investigation of muscle mitochondria. An exercise protocol was developed to study the load dependent changes in oxygenation of m. vastus lateralis of myopathic patients. On a bicycle ergometer exercise was done periodically. One period consisted of 1.5 min exercise followed by 3 min rest. Work load in the first period was 20 W, and was increased by 10 W for each subsequent period until maximal work load was reached. In 12 healthy volunteers we observed oxygenation of muscle during periods of low work load (warm-up effect). During periods of high work load the muscle deoxygenated. The work load at transition from oxygenation to deoxygenation (deoxygenation threshold) in controls was 75 W. In 3 patients with myopathies, in addition to NIR- spectroscopy, function of mitochondria of specimen of m. vastus lateralis was investigated biochemically. Muscle fibers were skinned with saponin and investigated with high resolution respirometry and multiple substrate-inhibitor- titration. Mitochondrial function was impaired in patients who had abnormal findings in NIR spectroscopy.

NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers

NASA Astrophysics Data System (ADS)

Gellerich, Frank N.; Mueller, Tobias; Nioka, Shoko; Hertel, Katrin; Schulte-Mattler, Wilhelm J.; Zierz, Stephan; Chance, Britton

1997-12-01

Noninvasive measurement of changes in oxygenation of human skeletal muscle can be done with a dual-wavelength near infrared (NIR) spectrophotometer. This allows a noninvasive investigation of muscle mitochondria. An exercise protocol was developed to study the load dependent changes in oxygenation of m. vastus lateralis of myopathic patients. On a bicycle ergometer exercise was done periodically. One period consisted of 1.5 min exercise followed by 3 min rest. Work load in the first period was 20 W, and was increased by 10 W for each subsequent period until maximal work load was reached. In 12 healthy volunteers we observed oxygenation of muscle during periods of low work load (warm-up effect). During periods of high work load the muscle deoxygenated. The work load at transition from oxygenation to deoxygenation (deoxygenation threshold) in controls was 75 W. In 3 patients with myopathies, in addition to NIR- spectroscopy, function of mitochondria of specimen of m. vastus lateralis was investigated biochemically. Muscle fibers were skinned with saponin and investigated with high resolution respirometry and multiple substrate-inhibitor- titration. Mitochondrial function was impaired in patients who had abnormal findings in NIR spectroscopy.

Newer views of the Moon: Comparing spectra from Clementine and the Moon Mineralogy Mapper

USGS Publications Warehouse

Kramer, G.Y.; Besse, S.; Nettles, J.; Combe, J.-P.; Clark, R.N.; Pieters, C.M.; Staid, M.; Malaret, E.; Boardman, J.; Green, R.O.; Head, J.W.; McCord, T.B.

2011-01-01

The Moon Mineralogy Mapper (M3) provided the first global hyperspectral data of the lunar surface in 85 bands from 460 to 2980 nm. The Clementine mission provided the first global multispectral maps the lunar surface in 11 spectral bands across the ultraviolet-visible (UV-VIS) and near-infrared (NIR). In an effort to understand how M3 improves our ability to analyze and interpret lunar data, we compare M3 spectra with those from Clementine's UV-VIS and NIR cameras. The Clementine mission provided the first global multispectral maps the lunar surface in 11 spectral bands across the UV-VIS and NIR. We have found that M3 reflectance values are lower across all wavelengths compared with albedos from both of Clementine's UV-VIS and NIR cameras. M3 spectra show the Moon to be redder, that is, have a steeper continuum slope, than indicated by Clementine. The 1 m absorption band depths may be comparable between the instruments, but Clementine data consistently exhibit shallower 2 m band depths than M 3. Absorption band minimums are difficult to compare due to the significantly different spectral resolutions. Copyright 2011 by the American Geophysical Union.

Newer views of the Moon: Comparing spectra from Clementineand the Moon Mineralogy Mapper

USGS Publications Warehouse

Georgiana Y. Kramer,; Sebastian Besse,; Nettles, Jeff; Jean-Philippe Combe,; Clark, Roger N.; Pieters, Carle M.; Matthew Staid,; Joseph Boardman,; Robert Green,; McCord, Thomas B.; Malaret, Erik; Head, James W.

2011-01-01

The Moon Mineralogy Mapper (M3) provided the first global hyperspectral data of the lunar surface in 85 bands from 460 to 2980 nm. The Clementine mission provided the first global multispectral maps the lunar surface in 11 spectral bands across the ultraviolet-visible (UV-VIS) and near-infrared (NIR). In an effort to understand how M3 improves our ability to analyze and interpret lunar data, we compare M3 spectra with those from Clementine's UV-VIS and NIR cameras. The Clementine mission provided the first global multispectral maps the lunar surface in 11 spectral bands across the UV-VIS and NIR. We have found that M3 reflectance values are lower across all wavelengths compared with albedos from both of Clementine's UV-VIS and NIR cameras. M3 spectra show the Moon to be redder, that is, have a steeper continuum slope, than indicated by Clementine. The 1 μm absorption band depths may be comparable between the instruments, but Clementine data consistently exhibit shallower 2 μm band depths than M3. Absorption band minimums are difficult to compare due to the significantly different spectral resolutions.

A novel near-infrared nanomaterial with high quantum efficiency and its applications in real time in vivo imaging.

PubMed

Cui, X X; Fan, Q; Shi, S J; Wen, W H; Chen, D F; Guo, H T; Xu, Y T; Gao, F; Nie, R Z; Ford, Harold D; Tang, Gordon H; Hou, C Q; Peng, B

2018-05-18

Fluorescence imaging signal is severely limited by the quantum efficiency and emission wavelength. To overcome these challenges, novel NIR-emitting K 5 NdLi 2 F 10 nanoparticles under NIR excitation was introduced as fluorescence imaging probe for the first time. The photostability of K 5 NdLi 2 F 10 nanoparticles in the water, phosphate buffer saline, fetal bovine serum and living mice was investigated. The fluorescence signal was detected with depths of 3.5 and 2.0 cm in phantom and pork tissue, respectively. Fluorescence spectrum with a significant signal-to-background ratio of 10:1 was captured in living mice. Moreover, clear NIR images were virtualized for the living mice after intravenous injection. The imaging ability of nanoparticles in tumor-beard mice were evaluated, the enrichment of K 5 NdLi 2 F 10 nanoparticles in tumor site due to the enhanced permeability and retention effect was confirmed. The systematic studies of toxicity, bio-distribution and in-vivo dynamic imaging suggest that these materials give high biocompatibility and low toxicity. These NIR-emitting nanoparticles with high quantum efficiency, high penetration and low toxicity might facilitate tumor identification in deep tissues more sensitively.

Intraoperative near-infrared autofluorescence imaging of parathyroid glands.

PubMed

Ladurner, Roland; Sommerey, Sandra; Arabi, Nora Al; Hallfeldt, Klaus K J; Stepp, Herbert; Gallwas, Julia K S

2017-08-01

To identify parathyroid glands intraoperatively by exposing their autofluorescence using near-infrared light. Fluorescence imaging was carried out during minimally invasive and open parathyroid and thyroid surgery. After identification, the parathyroid glands as well as the surrounding tissue were exposed to near-infrared (NIR) light with a wavelength of 690-770 nm using a modified Karl Storz near-infrared/indocyanine green (NIR/ICG) endoscopic system. Parathyroid tissue was expected to show near-infrared autofluorescence, captured in the blue channel of the camera. Whenever possible the visual identification of parathyroid tissue was confirmed histologically. In preliminary investigations, using the original NIR/ICG endoscopic system we noticed considerable interference of light in the blue channel overlying the autofluorescence. Therefore, we modified the light source by interposing additional filters. In a second series, we investigated 35 parathyroid glands from 25 patients. Twenty-seven glands were identified correctly based on NIR autofluorescence. Regarding the extent of autofluorescence, there were no noticeable differences between parathyroid adenomas, hyperplasia and normal parathyroid glands. In contrast, thyroid tissue, lymph nodes and adipose tissue revealed no substantial autofluorescence. Parathyroid tissue is characterized by showing autofluorescence in the near-infrared spectrum. This effect can be used to distinguish parathyroid glands from other cervical tissue entities.

In vivo photoacoustic tumor tomography using a quinoline-annulated porphyrin as NIR molecular contrast agent.

PubMed

Luciano, Michael; Erfanzadeh, Mohsen; Zhou, Feifei; Zhu, Hua; Bornhütter, Tobias; Röder, Beate; Zhu, Quing; Brückner, Christian

2017-01-25

The synthesis and photophysical properties of a tetra-PEG-modified and freely water-soluble quinoline-annulated porphyrin are described. We previously demonstrated the ability of quinoline-annulated porphyrins to act as an in vitro NIR photoacoustic imaging (PAI) contrast agent. The solubility of the quinoline-annulated porphyrin derivative in serum now allowed the assessment of the efficacy of the PEGylated derivative as an in vivo NIR contrast agent for the PAI of an implanted tumor in a mouse model. A multi-fold contrast enhancement when compared to the benchmark dye ICG could be shown, a finding that could be traced to its photophysical properties (short triplet lifetimes, low fluorescence and singlet oxygen sensitization quantum yields). A NIR excitation wavelength of 790 nm could be used, fully taking advantage of the optical window of tissue. Rapid renal clearance of the dye was observed. Its straight-forward synthesis, optical properties with the possibility for further optical fine-tuning, nontoxicity, favorable elimination rates, and contrast enhancement make this a promising PAI contrast agent. The ability to conjugate the PAI chromophore with a fluorescent tag using a facile and general conjugation strategy was also demonstrated.

Detection of artificially ripened mango using spectrometric analysis

NASA Astrophysics Data System (ADS)

Mithun, B. S.; Mondal, Milton; Vishwakarma, Harsh; Shinde, Sujit; Kimbahune, Sanjay

2017-05-01

Hyperspectral sensing has been proven to be useful to determine the quality of food in general. It has also been used to distinguish naturally and artificially ripened mangoes by analyzing the spectral signature. However the focus has been on improving the accuracy of classification after performing dimensionality reduction, optimum feature selection and using suitable learning algorithm on the complete visible and NIR spectrum range data, namely 350nm to 1050nm. In this paper we focus on, (i) the use of low wavelength resolution and low cost multispectral sensor to reliably identify artificially ripened mango by selectively using the spectral information so that classification accuracy is not hampered at the cost of low resolution spectral data and (ii) use of visible spectrum i.e. 390nm to 700 nm data to accurately discriminate artificially ripened mangoes. Our results show that on a low resolution spectral data, the use of logistic regression produces an accuracy of 98.83% and outperforms other methods like classification tree, random forest significantly. And this is achieved by analyzing only 36 spectral reflectance data points instead of the complete 216 data points available in visual and NIR range. Another interesting experimental observation is that we are able to achieve more than 98% classification accuracy by selecting only 15 irradiance values in the visible spectrum. Even the number of data needs to be collected using hyper-spectral or multi-spectral sensor can be reduced by a factor of 24 for classification with high degree of confidence

Tissue oxygenation and haemodynamics measurement with spatially resolved NIRS

NASA Astrophysics Data System (ADS)

Zhang, Y.; Scopesi, F.; Serra, G.; Sun, J. W.; Rolfe, P.

2010-08-01

We describe the use of Near Infrared Spectroscopy (NIRS) for the non-invasive investigation of changes in haemodynamics and oxygenation of human peripheral tissues. The goal was to measure spatial variations of tissue NIRS oxygenation variables, namely deoxy-haemoglobin (HHb), oxy-haemoglobin (HbO2), total haemoglobin (HbT), and thereby to evaluate the responses of the peripheral circulation to imposed physiological challenges. We present a skinfat- muscle heterogeneous tissue model with varying fat thickness up to 15mm and a Monte Carlo simulation of photon transport within this model. The mean partial path length and the mean photon visit depth in the muscle layer were derived for different source-detector spacing. We constructed NIRS instrumentation comprising of light-emitting diodes (LED) as light sources at four wavelengths, 735nm, 760nm, 810nm and 850nm and sensitive photodiodes (PD) as the detectors. Source-detector spacing was varied to perform measurements at different depths within forearm tissue. Changes in chromophore concentration in response to venous and arterial occlusion were calculated using the modified Lambert-Beer Law. Studies in fat and thin volunteers indicated greater sensitivity in the thinner subjects for the tissue oxygenation measurement in the muscle layer. These results were consistent with those found using Monte Carlo simulation. Overall, the results of this investigation demonstrate the usefulness of the NIRS instrument for deriving spatial information from biological tissues.

Non-destructive clinical assessment of occlusal caries lesions using near-IR imaging methods.

PubMed

Staninec, Michal; Douglas, Shane M; Darling, Cynthia L; Chan, Kenneth; Kang, Hobin; Lee, Robert C; Fried, Daniel

2011-12-01

Enamel is highly transparent in the near-IR (NIR) at wavelengths near 1,300 nm, and stains are not visible. The purpose of this study was to use NIR transillumination and optical coherence tomography (OCT) to estimate the severity of caries lesions on occlusal surfaces both in vivo and on extracted teeth. Extracted molars with suspected occlusal lesions were examined with OCT and polarization sensitive OCT (PS-OCT), and subsequently sectioned and examined with polarized light microscopy (PLM) and transverse microradiography (TMR). Teeth in test subjects with occlusal caries lesions that were not cavitated or visible on radiographs were examined using NIR transillumination at 1,310 nm using a custom built probe attached to an indium gallium arsenide (InGaAs) camera and a linear OCT scanner. After imaging, cavities were prepared using dye staining to guide caries removal and physical impressions of the cavities were taken. The lesion severity determined from OCT and PS-OCT scans in vitro correlated with the depth determined using PLM and TMR. Occlusal caries lesions appeared in NIR images with high contrast in vivo. OCT scans showed that most of the lesions penetrated to dentin and spread laterally below the sound enamel. This study demonstrates that both NIR transillumination and OCT are promising new methods for the clinical diagnosis of occlusal caries. Copyright © 2011 Wiley Periodicals, Inc.

Nondestructive Clinical Assessment of Occlusal Caries Lesions using Near-IR Imaging Methods

PubMed Central

Staninec, Michal; Douglas, Shane M.; Darling, Cynthia L.; Chan, Kenneth; Kang, Hobin; Lee, Robert C.; Fried, Daniel

2011-01-01

Objective Enamel is highly transparent in the near-IR (NIR) at wavelengths near 1300-nm, and stains are not visible. The purpose of this study was to use NIR transillumination and optical coherence tomography (OCT) to estimate the severity of caries lesions on occlusal surfaces both in vivo and on extracted teeth. Methods Extracted molars with suspected occlusal lesions were examined with OCT and polarization sensitive OCT (PS-OCT), and subsequently sectioned and examined with polarized light microscopy (PLM) and transverse microradiography (TMR). Teeth in test subjects with occlusal caries lesions that were not cavitated or visible on radiographs were examined using NIR transillumination at 1310 nm using a custom built probe attached to an indium gallium arsenide (InGaAs) camera and a linear OCT scanner. After imaging, cavities were prepared using dye staining to guide caries removal and physical impressions of the cavities were taken. Results The lesion severity determined from OCT and PS-OCT scans in vitro correlated with the depth determined using polarized light microscopy (PLM) and transverse microradiography (TMR). Occlusal caries lesions appeared in NIR images with high contrast in vivo. OCT scans showed that most of the lesions penetrated to dentin and spread laterally below the sound enamel. Conclusion This study demonstrates that both NIR transillumination and OCT are promising new methods for the clinical diagnosis of occlusal caries. PMID:22109697

Boosting the down-shifting luminescence of rare-earth nanocrystals for biological imaging beyond 1500 nm.

PubMed

Zhong, Yeteng; Ma, Zhuoran; Zhu, Shoujun; Yue, Jingying; Zhang, Mingxi; Antaris, Alexander L; Yuan, Jie; Cui, Ran; Wan, Hao; Zhou, Ying; Wang, Weizhi; Huang, Ngan F; Luo, Jian; Hu, Zhiyuan; Dai, Hongjie

2017-09-29

In vivo fluorescence imaging in the near-infrared region between 1500-1700 nm (NIR-IIb window) affords high spatial resolution, deep-tissue penetration, and diminished auto-fluorescence due to the suppressed scattering of long-wavelength photons and large fluorophore Stokes shifts. However, very few NIR-IIb fluorescent probes exist currently. Here, we report the synthesis of a down-conversion luminescent rare-earth nanocrystal with cerium doping (Er/Ce co-doped NaYbF 4 nanocrystal core with an inert NaYF 4 shell). Ce doping is found to suppress the up-conversion pathway while boosting down-conversion by ~9-fold to produce bright 1550 nm luminescence under 980 nm excitation. Optimization of the inert shell coating surrounding the core and hydrophilic surface functionalization minimize the luminescence quenching effect by water. The resulting biocompatible, bright 1550 nm emitting nanoparticles enable fast in vivo imaging of blood vasculature in the mouse brain and hindlimb in the NIR-IIb window with short exposure time of 20 ms for rare-earth based probes.Fluorescence imaging in the near-infrared window between 1500-1700 nm (NIR-IIb window) offers superior spatial resolution and tissue penetration depth, but few NIR-IIb probes exist. Here, the authors synthesize rare earth down-converting nanocrystals as promising fluorescent probes for in vivo imaging in this spectral region.

Ventrolateral prefrontal activation during a N-back task assessed with multichannel functional near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Zhou, Yuan; Zhu, Ye; Jiang, Tianzi

2007-05-01

Functional near-infrared spectroscopy (fNIRS) has been used to investigate the changes in the concentration of oxygenated (O2Hb) and deoxygenated (HHb) hemoglobin in brain issue during several cognitive tasks. In the present study, by means of multichannel dual wavelength light-emitting diode continuous-wave (CW) NIRS, we investigated the blood oxygenation changes of prefrontal cortex in 18 healthy subjects while performing a verbal n-back task (0-back and 2-back), which has been rarely investigated by fNIRS. Compared to the 0-back task (control task), we found a significant increase of O2Hb and total amount of hemoglobin (THb) in left and right ventrolateral prefrontal cortex (VLPFC) during the execution of the 2-back task compared to the 0-back task (p<0.05, FDR corrected). This result is consistent with the previous functional neuroimaging studies that have found the VLPFC activation related to verbal working memory. However, we found no significant hemisphere dominance. In addition, the effects of gender and its interaction with task performance on O2Hb concentration change were suggested in the present study. Our findings not only confirm that multichannel fNIRS is suitable to detect spatially specific activation during the performance of cognitive tasks; but also suggest that it should be cautious of gender-dependent difference in cerebral activation when interpreting the fNIRS data during cognitive tasks.

Near-infrared image-guided laser ablation of artificial caries lesions.

PubMed

Tao, You-Chen; Fan, Kenneth; Fried, Daniel

2007-01-01

Laser removal of dental hard tissue can be combined with optical, spectral or acoustic feedback systems to selectively ablate dental caries and restorative materials. Near-infrared (NIR) imaging has considerable potential for the optical discrimination of sound and demineralized tissue. The objective of this study was to test the hypothesis that two-dimensional NIR images of demineralized tooth surfaces can be used to guide CO(2) laser ablation for the selective removal of artificial caries lesions. Highly patterned artificial lesions were produced by submerging 5 × 5 mm(2) bovine enamel samples in demineralized solution for a 9-day period while sound areas were protected with acid resistant varnish. NIR imaging and polarization sensitive optical coherence tomography (PS-OCT) were used to acquire depth-resolved images at a wavelength of 1310-nm. An imaging processing module was developed to analyze the NIR images and to generate optical maps. The optical maps were used to control a CO(2) laser for the selective removal of the lesions at a uniform depth. This experiment showed that the patterned artificial lesions were removed selectively using the optical maps with minimal damage to sound enamel areas. Post-ablation NIR and PS-OCT imaging confirmed that demineralized areas were removed while sound enamel was conserved. This study successfully demonstrated that near-IR imaging can be integrated with a CO(2) laser ablation system for the selective removal of dental caries.

Near-infrared image-guided laser ablation of artificial caries lesions

PubMed Central

Tao, You-Chen; Fan, Kenneth; Fried, Daniel

2012-01-01

Laser removal of dental hard tissue can be combined with optical, spectral or acoustic feedback systems to selectively ablate dental caries and restorative materials. Near-infrared (NIR) imaging has considerable potential for the optical discrimination of sound and demineralized tissue. The objective of this study was to test the hypothesis that two–dimensional NIR images of demineralized tooth surfaces can be used to guide CO2 laser ablation for the selective removal of artificial caries lesions. Highly patterned artificial lesions were produced by submerging 5 × 5 mm2 bovine enamel samples in demineralized solution for a 9-day period while sound areas were protected with acid resistant varnish. NIR imaging and polarization sensitive optical coherence tomography (PS-OCT) were used to acquire depth-resolved images at a wavelength of 1310-nm. An imaging processing module was developed to analyze the NIR images and to generate optical maps. The optical maps were used to control a CO2 laser for the selective removal of the lesions at a uniform depth. This experiment showed that the patterned artificial lesions were removed selectively using the optical maps with minimal damage to sound enamel areas. Post-ablation NIR and PS-OCT imaging confirmed that demineralized areas were removed while sound enamel was conserved. This study successfully demonstrated that near-IR imaging can be integrated with a CO2 laser ablation system for the selective removal of dental caries. PMID:22866210

Near-infrared image-guided laser ablation of artificial caries lesions

NASA Astrophysics Data System (ADS)

Tao, You-Chen; Fan, Kenneth; Fried, Daniel

2007-02-01

Laser removal of dental hard tissue can be combined with optical, spectral or acoustic feedback systems to selectively ablate dental caries and restorative materials. Near-infrared (NIR) imaging has considerable potential for the optical discrimination of sound and demineralized tissue. The objective of this study was to test the hypothesis that two-dimensional NIR images of demineralized tooth surfaces can be used to guide CO II laser ablation for the selective removal of artificial caries lesions. Highly patterned artificial lesions were produced by submerging 5 x 5 mm2 bovine enamel samples in demineralized solution for a 9-day period while sound areas were protected with acid resistant varnish. NIR imaging and polarization sensitive optical coherence tomography (PS-OCT) were used to acquire depth-resolved images at a wavelength of 1310-nm. An imaging processing module was developed to analyze the NIR images and to generate optical maps. The optical maps were used to control a CO II laser for the selective removal of the lesions at a uniform depth. This experiment showed that the patterned artificial lesions were removed selectively using the optical maps with minimal damage to sound enamel areas. Post-ablation NIR and PS-OCT imaging confirmed that demineralized areas were removed while sound enamel was conserved. This study successfully demonstrated that near-IR imaging can be integrated with a CO II laser ablation system for the selective removal of dental caries.

Variable selection in near-infrared spectroscopy: benchmarking of feature selection methods on biodiesel data.

PubMed

Balabin, Roman M; Smirnov, Sergey V

2011-04-29

During the past several years, near-infrared (near-IR/NIR) spectroscopy has increasingly been adopted as an analytical tool in various fields from petroleum to biomedical sectors. The NIR spectrum (above 4000 cm(-1)) of a sample is typically measured by modern instruments at a few hundred of wavelengths. Recently, considerable effort has been directed towards developing procedures to identify variables (wavelengths) that contribute useful information. Variable selection (VS) or feature selection, also called frequency selection or wavelength selection, is a critical step in data analysis for vibrational spectroscopy (infrared, Raman, or NIRS). In this paper, we compare the performance of 16 different feature selection methods for the prediction of properties of biodiesel fuel, including density, viscosity, methanol content, and water concentration. The feature selection algorithms tested include stepwise multiple linear regression (MLR-step), interval partial least squares regression (iPLS), backward iPLS (BiPLS), forward iPLS (FiPLS), moving window partial least squares regression (MWPLS), (modified) changeable size moving window partial least squares (CSMWPLS/MCSMWPLSR), searching combination moving window partial least squares (SCMWPLS), successive projections algorithm (SPA), uninformative variable elimination (UVE, including UVE-SPA), simulated annealing (SA), back-propagation artificial neural networks (BP-ANN), Kohonen artificial neural network (K-ANN), and genetic algorithms (GAs, including GA-iPLS). Two linear techniques for calibration model building, namely multiple linear regression (MLR) and partial least squares regression/projection to latent structures (PLS/PLSR), are used for the evaluation of biofuel properties. A comparison with a non-linear calibration model, artificial neural networks (ANN-MLP), is also provided. Discussion of gasoline, ethanol-gasoline (bioethanol), and diesel fuel data is presented. The results of other spectroscopic techniques application, such as Raman, ultraviolet-visible (UV-vis), or nuclear magnetic resonance (NMR) spectroscopies, can be greatly improved by an appropriate feature selection choice. Copyright © 2011 Elsevier B.V. All rights reserved.

Determination of reference values for optical properties of liquid phantoms based on Intralipid and India ink

PubMed Central

Spinelli, L.; Botwicz, M.; Zolek, N.; Kacprzak, M.; Milej, D.; Sawosz, P.; Liebert, A.; Weigel, U.; Durduran, T.; Foschum, F.; Kienle, A.; Baribeau, F.; Leclair, S.; Bouchard, J.-P.; Noiseux, I.; Gallant, P.; Mermut, O.; Farina, A.; Pifferi, A.; Torricelli, A.; Cubeddu, R.; Ho, H.-C.; Mazurenka, M.; Wabnitz, H.; Klauenberg, K.; Bodnar, O.; Elster, C.; Bénazech-Lavoué, M.; Bérubé-Lauzière, Y.; Lesage, F.; Khoptyar, D.; Subash, A. A.; Andersson-Engels, S.; Di Ninni, P.; Martelli, F.; Zaccanti, G.

2014-01-01

A multi-center study has been set up to accurately characterize the optical properties of diffusive liquid phantoms based on Intralipid and India ink at near-infrared (NIR) wavelengths. Nine research laboratories from six countries adopting different measurement techniques, instrumental set-ups, and data analysis methods determined at their best the optical properties and relative uncertainties of diffusive dilutions prepared with common samples of the two compounds. By exploiting a suitable statistical model, comprehensive reference values at three NIR wavelengths for the intrinsic absorption coefficient of India ink and the intrinsic reduced scattering coefficient of Intralipid-20% were determined with an uncertainty of about 2% or better, depending on the wavelength considered, and 1%, respectively. Even if in this study we focused on particular batches of India ink and Intralipid, the reference values determined here represent a solid and useful starting point for preparing diffusive liquid phantoms with accurately defined optical properties. Furthermore, due to the ready availability, low cost, long-term stability and batch-to-batch reproducibility of these compounds, they provide a unique fundamental tool for the calibration and performance assessment of diffuse optical spectroscopy instrumentation intended to be used in laboratory or clinical environment. Finally, the collaborative work presented here demonstrates that the accuracy level attained in this work for optical properties of diffusive phantoms is reliable. PMID:25071947

Diamond encapsulated photovoltaics for transdermal power delivery.

PubMed

Ahnood, A; Fox, K E; Apollo, N V; Lohrmann, A; Garrett, D J; Nayagam, D A X; Karle, T; Stacey, A; Abberton, K M; Morrison, W A; Blakers, A; Prawer, S

2016-03-15

A safe, compact and robust means of wireless energy transfer across the skin barrier is a key requirement for implantable electronic devices. One possible approach is photovoltaic (PV) energy delivery using optical illumination at near infrared (NIR) wavelengths, to which the skin is highly transparent. In the work presented here, a subcutaneously implantable silicon PV cell, operated in conjunction with an external NIR laser diode, is developed as a power delivery system. The biocompatibility and long-term biostability of the implantable PV is ensured through the use of an hermetic container, comprising a transparent diamond capsule and platinum wire feedthroughs. A wavelength of 980 nm is identified as the optimum operating point based on the PV cell's external quantum efficiency, the skin's transmission spectrum, and the wavelength dependent safe exposure limit of the skin. In bench-top experiments using an external illumination intensity of 0.7 W/cm(2), a peak output power of 2.7 mW is delivered to the implant with an active PV cell dimension of 1.5 × 1.5 × 0.06 mm(3). This corresponds to a volumetric power output density of ~20 mW/mm(3), significantly higher than power densities achievable using inductively coupled coil-based approaches used in other medical implant systems. This approach paves the way for further ministration of bionic implants. Copyright © 2015 Elsevier B.V. All rights reserved.

Growth, optical, ICP and thermal studies of nonlinear optical single crystal: Sodium acid phthalate (NaAP)

NASA Astrophysics Data System (ADS)

Mahadevan, M.; Arivanandhan, M.; Elangovan, K.; Anandan, P.; Ramachandran, K.

2017-07-01

Good quality single crystals of sodium acid phthalate (NaAP) were grown by slow evaporation technique. Single crystal X-ray diffraction study of the grown crystal reveals that the crystal belongs to orthorhombic system with space group B2ab. Fourier transform infrared spectrum confirms the presence of the functional groups of the grown material. Inductively coupled plasma emission spectroscopy analysis is used to confirm the presence of Na element in the sample. Thermal analysis of the NaAP crystal shows that the crystal is stable up to 140°C. Optical transmittance of the grown crystal was recorded in the wavelength range from 200 and 800 nm using UV-Vis-NIR spectrophotometer. The second harmonic generation of NaAP was analysed using Kurtz powder technique.

Construction of Hierarchical Polymer Brushes on Upconversion Nanoparticles via NIR-Light-Initiated RAFT Polymerization.

PubMed

Xie, Zhongxi; Deng, Xiaoran; Liu, Bei; Huang, Shanshan; Ma, Pingan; Hou, Zhiyao; Cheng, Ziyong; Lin, Jun; Luan, Shifang

2017-09-13

Photoinduced reversible addition-fragmentation chain transfer (RAFT) polymerization generally adopts high-energy ultraviolet (UV) or blue light. In combination with photoredox catalyst, the excitation light wavelength was extended to the visible and even near-infrared (NIR) region for photoinduced electron transfer RAFT polymerization. In this report, we introduce for the first time a surface NIR-light-initiated RAFT polymerization on upconversion nanoparticles (UCNPs) without adding any photocatalyst and construct a functional inorganic core/polymer shell nanohybrid for application in cancer theranostics. The multilayer core-shell UCNPs (NaYF 4 :Yb/Tm@NaYbF 4 :Gd@NaNdF 4 :Yb@NaYF 4 ), with surface anchorings of chain transfer agents, can serve as efficient NIR-to-UV light transducers for initiating the RAFT polymerization. A hierarchical double block copolymer brush, consisting of poly(acrylic acid) (PAA) and poly(oligo(ethylene oxide)methacrylate-co-2-(2-methoxy-ethoxy)ethyl methacrylate) (PEG for short), was grafted from the surface in sequence. The targeting arginine-glycine-aspartic (RGD) peptide was modified at the end of the copolymer through the trithiolcarbonate end group. After loading of doxorubicin, the UCNPs@PAA-b-PEG-RGD exhibited an enhanced U87MG cancer cell uptake efficiency and cytotoxicity. Besides, the unique upconversion luminescence of the nanohybrids was used for the autofluoresence-free cell imaging and labeling. Therefore, our strategy verified that UCNPs could efficiently activate RAFT polymerization by NIR photoirradiation and construct the complex nanohybrids, exhibiting prospective biomedical applications due to the low phototoxicity and deep penetration of NIR light.

New laser design for NIR lidar applications

NASA Astrophysics Data System (ADS)

Vogelmann, H.; Trickl, T.; Perfahl, M.; Biggel, S.

2018-04-01

Recently, we quantified the very high spatio-temporal short term variability of tropospheric water vapor in a three dimensional study [1]. From a technical point of view this also depicted the general requirement of short integration times for recording water-vapor profiles with lidar. For this purpose, the only suitable technique is the differential absorption lidar (DIAL) working in the near-infrared (NIR) spectral region. The laser emission of most water vapor DIAL systems is generated by Ti:sapphire or alexandrite lasers. The water vapor absorption band at 817 nm is predominated for the use of Ti:sapphire. We present a new concept of transversely pumping in a Ti:Sapphire amplification stage as well as a compact laser design for the generation of single mode NIR pulses with two different DIAL wavelengths inside a single resonator. This laser concept allows for high output power due to repetitions rates up to 100Hz or even more. It is, because of its compactness, also suitable for mobile applications.

Near-infrared-to-near-infrared down-shifting and upconversion luminescence of KY{sub 3}F{sub 10} with single dopant of Nd{sup 3+} ion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Huihong; State Key Laboratory of Luminescence Materials and Devices and Institute of Optical Communication Materials, South China University of Technology, Guangzhou 510641; Yu, Ting

2016-01-25

We have studied the structural and near-infrared (NIR) luminescent properties of KY{sub 3}F{sub 10} phosphors, singly doped with Nd{sup 3+} serving as both sensitizer and activator. With a single laser diode at the wavelength of 808 nm as a pump source, simultaneous NIR-to-NIR upconversion (UC) and down-shifting (DS) emissions are effectively achieved, due to the specific energy levels of Nd{sup 3+} dopant and the low phonon energy of the host. The luminescence mechanism related to energy transfer is discussed. The luminescence can be modulated through controlling the population of Nd{sup 3+}:{sup 4}F{sub 3/2} state in our experiment. Interestingly, both UC andmore » DS emissions of the material fall within the dual biological window, suggesting that the prepared phosphors have potential applications in the bioimaging field.« less

Study on nondestructive discrimination of genuine and counterfeit wild ginsengs using NIRS

NASA Astrophysics Data System (ADS)

Lu, Q.; Fan, Y.; Peng, Z.; Ding, H.; Gao, H.

2012-07-01

A new approach for the nondestructive discrimination between genuine wild ginsengs and the counterfeit ones by near infrared spectroscopy (NIRS) was developed. Both discriminant analysis and back propagation artificial neural network (BP-ANN) were applied to the model establishment for discrimination. Optimal modeling wavelengths were determined based on the anomalous spectral information of counterfeit samples. Through principal component analysis (PCA) of various wild ginseng samples, genuine and counterfeit, the cumulative percentages of variance of the principal components were obtained, serving as a reference for principal component (PC) factor determination. Discriminant analysis achieved an identification ratio of 88.46%. With sample' truth values as its outputs, a three-layer BP-ANN model was built, which yielded a higher discrimination accuracy of 100%. The overall results sufficiently demonstrate that NIRS combined with BP-ANN classification algorithm performs better on ginseng discrimination than discriminant analysis, and can be used as a rapid and nondestructive method for the detection of counterfeit wild ginsengs in food and pharmaceutical industry.

Use of visible and near infrared reflectance spectra to predict lipid peroxidation of light lamb meat and discriminate dam's feeding systems.

PubMed

Ripoll, G; Lobón, S; Joy, M

2018-09-01

Measurement of thiobarbituric acid reactive substances (TBARS) is a well-established method for determine lipid oxidation in meat. This assay, however, is time-consuming and generates undesired chemical waste. Dam's milk is the principal source of vitamins and provitamins that delay lipid oxidation of light lamb meat; these compounds are stored in the lamb's muscle tissue. Hence, lamb meat could be used to determine the origin of the dam's diet. The aim of this study is to evaluate Near-infrared reflectance spectroscopy (NIRS) as a tool for determining the lipid peroxidation of light lamb meat and differentiate the meat of light lambs according the diet of their dams during lactation (grazing alfalfa, lucerne, or fed a total mixed ration). NIRS using select wavelengths was able to detect the lipid oxidation of meat (TBARS method). NIRS can detect analytes at concentrations of parts per million. Moreover, the feed diets were discriminated successfully. Copyright © 2018 Elsevier Ltd. All rights reserved.

Planning the 8-meter Chinese Giant Solar Telescope

NASA Astrophysics Data System (ADS)

Beckers, Jacques M.; Liu, Z.; Deng, Y.; Ji, H.

2013-07-01

The Chinese Giant Solar Telescope (CGST) will be a diffraction limited solar telescope optimized for the near-infrared (NIR) spectral region (0.8 - 2.5 microns). Its diffraction limit will be reached by the incorporation of Multi-Conjugate Adaptive Optics (MCAO) enhanced by image restoration techniques to achieve uniform (u.v) plane coverage over the angular spatial frequency region allowed by its 8-meter aperture. Thus it will complement the imaging capabilities of 4-meter telescopes being planned elsewhere which are optimized for the visible (VIS) spectral region (300 - 1000 nm) In the NIR spectral regions the CGST will have access to unique spectral features which will improve the diagnostics of the solar atmosphere. These include the CaII lines near 860 nm , the HeI lines near 1083 nm, the 1074 nm FeXIII coronal lines, the large Zeeman-split FeI line at 1548 nm, and (v) the H- continuum absorption minimum at 1.6 micron. Especially in sunspot umbrae the simultaneous observation of continua and lines across the NIR spectral range will cover a substantial depth range in the solar atmosphere. Of course the mid- and far- infrared regions are also available for unequalled high-angular resolution solar observations, for example, in the Hydrogen Bracket lines, CO molecular bands, and the MgI emission line at 12.3 microns. The CGST is a so-called ring telescope in which the light is captured by a 1 meter wide segmented ring or by a ring of 7 smaller off-axis aperture telescopes. The open central area of the telescope is large. The advantages of such a ring configuration is that (a) it covers all the spatial frequencies out to those corresponding to its outer diameter, (b) its circular symmetry makes it polarization neutral, (c) its large central hole helps thermal control, and (d) it provides ample space for the MCAO system and instrumentation in the Gregorian focus. Even though optimized for the NIR, we expect to use the CGST also at visible wavelengths in the so-called “Partial Adaptive Optics” (PAO) mode (Applied Optics 31,424,1992) to obtain angular resolution twice that of a 4-meter telescope if their observations indicate that higher resolution is desirable. The CGST is a Chinese solar community project.

Smart Gold Nanobowls (Nano-Crescent Moon) with Sub-10 nm Circular Edge for Local Electromagnetic Field Enhancement Effect, Spatial, and NIR Temporal/Thermal Modulations for Molecular and Cellular Dynamic Imaging

DTIC Science & Technology

2006-01-31

nanoring [10], the Au nanocrescent has a higher local field enhancement factor in the near infrared wavelength region due to the simultaneous...incorporation of SERS hot spots including sharp nanotip and nanoring geometries and thus the strong hybrid resonance modes from nanocavity resonance mode and tip...Raman, "A change of wave-length in light scattering," Nature 121, 619-619 (1928). 22. Y. Lu, G. L. Liu, and L. P. Lee, "High-density silver nanoparticle

Recent Advances of Light-Mediated Theranostics

PubMed Central

Ai, Xiangzhao; Mu, Jing; Xing, Bengang

2016-01-01

Currently, precision theranostics have been extensively demanded for the effective treatment of various human diseases. Currently, efficient therapy at the targeted disease areas still remains challenging since most available drug molecules lack of selectivity to the pathological sites. Among different approaches, light-mediated therapeutic strategy has recently emerged as a promising and powerful tool to precisely control the activation of therapeutic reagents and imaging probes in vitro and in vivo, mostly attributed to its unique properties including minimally invasive capability and highly spatiotemporal resolution. Although it has achieved initial success, the conventional strategies for light-mediated theranostics are mostly based on the light with short wavelength (e.g., UV or visible light), which may usually suffer from several undesired drawbacks, such as limited tissue penetration depth, unavoidable light absorption/scattering and potential phototoxicity to healthy tissues, etc. Therefore, a near-infrared (NIR) light-mediated approach on the basis of long-wavelength light (700-1000 nm) irradiation, which displays deep-tissue penetration, minimized photo-damage and low autofluoresence in living systems, has been proposed as an inspiring alternative for precisely phototherapeutic applications in the last decades. Despite numerous NIR light-responsive molecules have been currently proposed for clinical applications, several inherent drawbacks, such as troublesome synthetic procedures, low water solubility and limited accumulation abilities in targeted areas, heavily restrict their applications in deep-tissue therapeutic and imaging studies. Thanks to the amazing properties of several nanomaterials with large extinction coefficient in the NIR region, the construction of NIR light responsive nanoplatforms with multifunctions have become promising approaches for deep-seated diseases diagnosis and therapy. In this review, we summarized various light-triggered theranostic strategies and introduced their great advances in biomedical applications in recent years. Moreover, some other promising light-assisted techniques, such as photoacoustic and Cerenkov radiation, were also systemically discussed. Finally, the potential challenges and future perspectives for light-mediated deep-tissue diagnosis and therapeutics were proposed. PMID:27877246

OISTER optical and near-infrared observations of the super-Chandrasekhar supernova candidate SN 2012dn: Dust emission from the circumstellar shell

NASA Astrophysics Data System (ADS)

Yamanaka, Masayuki; Maeda, Keiichi; Tanaka, Masaomi; Tominaga, Nozomu; Kawabata, Koji S.; Takaki, Katsutoshi; Kawabata, Miho; Nakaoka, Tatsuya; Ueno, Issei; Akitaya, Hiroshi; Nagayama, Takahiro; Takahashi, Jun; Honda, Satoshi; Omodaka, Toshihiro; Miyanoshita, Ryo; Nagao, Takashi; Watanabe, Makoto; Isogai, Mizuki; Arai, Akira; Itoh, Ryosuke; Ui, Takahiro; Uemura, Makoto; Yoshida, Michitoshi; Hanayama, Hidekazu; Kuroda, Daisuke; Ukita, Nobuharu; Yanagisawa, Kenshi; Izumiura, Hideyuki; Saito, Yoshihiko; Masumoto, Kazunari; Ono, Rikako; Noguchi, Ryo; Matsumoto, Katsura; Nogami, Daisaku; Morokuma, Tomoki; Oasa, Yumiko; Sekiguchi, Kazuhiro

2016-10-01

We present extensively dense observations of the super-Chandrasekhar supernova (SC SN) candidate SN 2012dn from -11 to +140 d after the date of its B-band maximum in the optical and near-infrared (NIR) wavelengths conducted through the OISTER ToO (Optical and Infrared Synergetic Telescopes for Education and Research Target of Opportunity) program. The NIR light curves and color evolutions up to 35 days after the B-band maximum provided an excellent match with those of another SC SN 2009dc, providing further support to the nature of SN 2012dn as an SC SN. We found that SN 2012dn exhibited strong excesses in the NIR wavelengths from 30 d after the B-band maximum. The H- and Ks-band light curves exhibited much later maximum dates at 40 and 70 d after the B-band maximum, respectively, compared with those of normal SNe Ia. The H- and Ks-band light curves subtracted by those of SN 2009dc displayed plateaued evolutions, indicating an NIR echo from the surrounding dust. The distance to the inner boundary of the dust shell is limited to 4.8-6.4 × 10-2 pc. No emission lines were found in its early phase spectra, suggesting that the ejecta-circumstellar material interaction could not occur. On the other hand, we found no signature that strongly supports the scenario of dust formation. The mass-loss rate of the pre-explosion system is estimated to be 10-6-10-5 M⊙ yr-1, assuming that the wind velocity of the system is 10-100 km s-1, which suggests that the progenitor of SN 2012dn could be a recurrent nova system. We conclude that the progenitor of this SC SN could be explained by the single-degenerate scenario.

High-Resolution Infrared Filter System for Solar Spectroscopy and Polarimetry

NASA Astrophysics Data System (ADS)

Cao, W.; Ma, J.; Wang, J.; Goode, P. R.; Wang, H.; Denker, C.

2003-05-01

We report on the design of an imaging filter system working at the near infrared (NIR) of 1.56 μ m to obtain monochromatic images and to probe weak magnetic fields in different layers of the deep photosphere with high temporal resolution and spatial resolution at Big Bear Solar Observatory (BBSO). This filter system consists of an interference filter, a birefringent filter, and a Fabry-Pérot etalon. As the narrowest filter system, the infrared Fabry-Pérot plays an important role in achieving narrow band transmission and high throughput, maintaining wavelength tuning ability, and assuring stability and reliability. In this poster, we outline a set of methods for the evaluation and calibration of the near infrared Fabry-Pérot etalon. Two-dimensional characteristic maps of the near infrared Fabry-Pérot etalon, including full-width-at-half-maximum (FWHM), effective finesse, peak transmission, along with free spectral range, flatness, roughness, stability and repeatability were obtained with lab equipments. Finally, by utilizing these results, a detailed analysis of the filter performance for the Fe I 1.5648 μ m and Fe I 1.5652 μ m Zeeman sensitive lines is presented. These results will benefit the design of NIR spectro-polarimeter of Advanced Technology Solar Telescope (ATST).

Rapid monitoring of grape withering using visible near-infrared spectroscopy.

PubMed

Beghi, Roberto; Giovenzana, Valentina; Marai, Simone; Guidetti, Riccardo

2015-12-01

Wineries need new practical and quick instruments, non-destructive and able to quantitatively evaluate during withering the parameters that impact product quality. The aim of the work was to test an optical portable system (visible near-infrared (NIR) spectrophotometer) in a wavelength range of 400-1000 nm for the prediction of quality parameters of grape berries during withering. A total of 300 red grape samples (Vitis vinifera L., Corvina cultivar) harvested in vintage year 2012 from the Valpolicella area (Verona, Italy) were analyzed. Qualitative (principal component analysis, PCA) and quantitative (partial least squares regression algorithm, PLS) evaluations were performed on grape spectra. PCA showed a clear sample grouping for the different withering stages. PLS models gave encouraging predictive capabilities for soluble solids content (R(2) val  = 0.62 and ratio performance deviation, RPD = 1.87) and firmness (R(2) val  = 0.56 and RPD = 1.79). The work demonstrated the applicability of visible NIR spectroscopy as a rapid technique for the analysis of grape quality directly in barns, during withering. The sector could be provided with simple and inexpensive optical systems that could be used to monitor the withering degree of grape for better management of the wine production process. © 2014 Society of Chemical Industry.

Near-infrared spectral methods for noninvasively measuring blood glucose

NASA Astrophysics Data System (ADS)

Fei, Sun; Kong, Deyi; Mei, Tao; Tao, Yongchun

2004-05-01

Determination of blood glucose concentrations in diabetic patients is a frequently occurring procedure and an important tool for diabetes management. Use of noninvasive detection techniques can relieve patients from the pain of frequent finger pokes and avoid the infection of disease via blood. This thesis discusses current research and analyzes the advantages and shortages of different measurement methods, including: optical methods (Transmission, Polarimetry and scattering), then, we give emphasis to analyze the technology of near-infrared (NIR) spectra. NIR spectral range 700 nm ~2300 nm was used because of its good transparency for biological tissue and presence of glucose absorption band. In this work, we present an outline of noninvasive blood glucose measurement. A near-infrared light beam is passed through the finger, and the spectral components of the emergent beam are measured using spectroscopic techniques. The device includes light sources having the wavelengths of 600 nm - 1800 nm to illuminate the tissue. Receptors associated with the light sources for receiving light and generating a transmission signal representing the light transmitted are also provided. Once a transmission signal is received by receptors, and the high and low values from each of the signals are stored in the device. The averaged values are then analyzed to determine the glucose concentration, which is displayed on the device.

Light-emitting diode-based multiwavelength diffuse optical tomography system guided by ultrasound

PubMed Central

Yuan, Guangqian; Alqasemi, Umar; Chen, Aaron; Yang, Yi; Zhu, Quing

2014-01-01

Abstract. Laser diodes are widely used in diffuse optical tomography (DOT) systems but are typically expensive and fragile, while light-emitting diodes (LEDs) are cheaper and are also available in the near-infrared (NIR) range with adequate output power for imaging deeply seated targets. In this study, we introduce a new low-cost DOT system using LEDs of four wavelengths in the NIR spectrum as light sources. The LEDs were modulated at 20 kHz to avoid ambient light. The LEDs were distributed on a hand-held probe and a printed circuit board was mounted at the back of the probe to separately provide switching and driving current to each LED. Ten optical fibers were used to couple the reflected light to 10 parallel photomultiplier tube detectors. A commercial ultrasound system provided simultaneous images of target location and size to guide the image reconstruction. A frequency-domain (FD) laser-diode-based system with ultrasound guidance was also used to compare the results obtained from those of the LED-based system. Results of absorbers embedded in intralipid and inhomogeneous tissue phantoms have demonstrated that the LED-based system provides a comparable quantification accuracy of targets to the FD system and has the potential to image deep targets such as breast lesions. PMID:25473884

Comparison of optimal wavelengths selection methods for visible/near-infrared prediction of apple firmness and soluble solids content

USDA-ARS?s Scientific Manuscript database

Visible and near-infrared (Vis-NIR) spectroscopy is now being used for nondestructive quality measurement of fruits and other food products. To implement the technology, it is necessary to develop an effective calibration model relating the acquired spectral data to the quality attribute(s) of inter...

Determination of Protein Content by NIR Spectroscopy in Protein Powder Mix Products.

PubMed

Ingle, Prashant D; Christian, Roney; Purohit, Piyush; Zarraga, Veronica; Handley, Erica; Freel, Keith; Abdo, Saleem

2016-01-01

Protein is a principal component in commonly used dietary supplements and health food products. The analysis of these products, within the consumer package form, is of critical importance for the purpose of ensuring quality and supporting label claims. A rapid test method was developed using near-infrared (NIR) spectroscopy as a compliment to current protein determination by the Dumas combustion method. The NIR method was found to be a rapid, low-cost, and green (no use of chemicals and reagents) complimentary technique. The protein powder samples analyzed in this study were in the range of 22-90% protein. The samples were prepared as mixtures of soy protein, whey protein, and silicon dioxide ingredients, which are common in commercially sold protein powder drink-mix products in the market. A NIR regression model was developed with 17 samples within the constituent range and was validated with 20 independent samples of known protein levels (85-88%). The results show that the NIR method is capable of predicting the protein content with a bias of ±2% and a maximum bias of 3% between NIR and the external Dumas method.

Ultra-compact MEMS FTIR spectrometer

NASA Astrophysics Data System (ADS)

Sabry, Yasser M.; Hassan, Khaled; Anwar, Momen; Alharon, Mohamed H.; Medhat, Mostafa; Adib, George A.; Dumont, Rich; Saadany, Bassam; Khalil, Diaa

2017-05-01

Portable and handheld spectrometers are being developed and commercialized in the late few years leveraging the rapidly-progressing technology and triggering new markets in the field of on-site spectroscopic analysis. Although handheld devices were commercialized for the near-infrared spectroscopy (NIRS), their size and cost stand as an obstacle against the deployment of the spectrometer as spectral sensing components needed for the smart phone industry and the IoT applications. In this work we report a chip-sized microelectromechanical system (MEMS)-based FTIR spectrometer. The core optical engine of the solution is built using a passive-alignment integration technique for a selfaligned MEMS chip; self-aligned microoptics and a single detector in a tiny package sized about 1 cm3. The MEMS chip is a monolithic, high-throughput scanning Michelson interferometer fabricated using deep reactive ion etching technology of silicon-on-insulator substrate. The micro-optical part is used for conditioning the input/output light to/from the MEMS and for further light direction to the detector. Thanks to the all-reflective design of the conditioning microoptics, the performance is free of chromatic aberration. Complemented by the excellent transmission properties of the silicon in the infrared region, the integrated solution allows very wide spectral range of operation. The reported sensor's spectral resolution is about 33 cm-1 and working in the range of 1270 nm to 2700 nm; upper limited by the extended InGaAs detector. The presented solution provides a low cost, low power, tiny size, wide wavelength range NIR spectral sensor that can be manufactured with extremely high volumes. All these features promise the compatibility of this technology with the forthcoming demand of smart portable and IoT devices.

Near-infrared spectroscopy for burning plasma diagnostic applications.

PubMed

Soukhanovskii, V A

2008-10-01

Ultraviolet and visible (UV-VIS, 200-750 nm) atomic spectroscopy of neutral and ionized fuel species (H, D, T, and Li) and impurities (e.g., He, Be, C, and W) is a key element of plasma control and diagnosis on International Thermonuclear Experimental Reactor and future magnetically confined burning plasma experiments (BPXs). Spectroscopic diagnostic implementation and performance issues that arise in the BPX harsh nuclear environment in the UV-VIS range, e.g., degradation of first mirror reflectivity under charge-exchange atom bombardment (erosion) and impurity deposition, permanent and dynamic loss of window, and optical fiber transmission under intense neutron and gamma-ray fluxes, are either absent or not as severe in the near-infrared (NIR, 750-2000 nm) range. An initial survey of NIR diagnostic applications has been undertaken on the National Spherical Torus Experiment. It is demonstrated that NIR spectroscopy can be used for machine protection and plasma control applications, as well as contribute to plasma performance evaluation and physics studies. Emission intensity estimates demonstrate that NIR measurements are possible in the BPX plasma operating parameter range. Complications in the NIR range due to the parasitic background emissions are expected to occur at very high plasma densities, low impurity densities, and at high plasma-facing component temperatures.

Multi-wavelength Characterization of Brown and Black Carbon from Filter Samples

NASA Astrophysics Data System (ADS)

Johnson, M. M.; Yatavelli, R. L. N.; Chen, L. W. A. A.; Gyawali, M. S.; Arnott, W. P.; Wang, X.; Chakrabarty, R. K.; Moosmüller, H.; Watson, J. G.; Chow, J. C.

2014-12-01

Particulate matter (PM) scatters and absorbs solar radiation and thereby affects visibility, the Earth's radiation balance, and properties and lifetimes of clouds. Understanding the radiative forcing (RF) of PM is essential to reducing the uncertainty in total anthropogenic and natural RF. Many instruments that measure light absorption coefficients (βabs [λ], Mm-1) of PM have used light at near-infrared (NIR; e.g., 880 nm) or red (e.g., 633 nm) wavelengths. Measuring βabs over a wider wavelength range, especially including the ultraviolet (UV) and visible, allows for contributions from black carbon (BC), brown carbon (BrC), and mineral dust (MD) to be differentiated. This will help to determine PM RF and its emission sources. In this study, source and ambient samples collected on Teflon-membrane and quartz-fiber filters are used to characterize and develop a multi-wavelength (250 - 1000 nm) filter-based measurement method of PM light absorption. A commercially available UV-visible spectrometer coupled with an integrating sphere is used for quantifying diffuse reflectance and transmittance of filter samples, from which βabs and absorption Ǻngström exponents (AAE) of the PM deposits are determined. The filter-based light absorption measurements of laboratory generated soot and biomass burning aerosol are compared to 3-wavelength photoacoustic absorption measurements to evaluate filter media and loading effects. Calibration factors are developed to account for differences between filter types (Teflon-membrane vs. quartz-fiber), and between filters and in situ photoacoustic absorption values. Application of multi-spectral absorption measurements to existing archived filters, including specific source samples (e.g. diesel and gasoline engines, biomass burning, dust), will also be discussed.

New MOEMS based systems appropriate for spectroscopic investigations on agricultural growth and perishable food conditions

NASA Astrophysics Data System (ADS)

Grueger, Heinrich; Schenk, Harald; Heberer, Andreas; Zimmer, Fabian; Scherff, Werner; Kenda, Andreas; Frank, Albert

2005-11-01

Further optimization of the agricultural growth process and quality control of perishable food which can be fruits and vegetables as well as every kind of meat or milk product requires new approaches for the sensitive front end. One possibility is reflectance or fluorescence spectroscopy in a wide wavelength range. By now broad usage is hindered by costs, size and performance of existing systems. MOEMS scanning gratings for spectrometers and translational mirrors for Fourier Transform spectroscopy enable small robust systems working in a range from 200nm to 5μm. Both types use digital signal processors (DSPs) capable to compute the spectra and execute complex evaluation and decision algorithms. The MOEMS chips are realized by anisotropic etching of a silicon on insulator (SOI) substrate. First the backside silicon and buried oxide is removed by a wet process then the front side structure is realized by dry etching. Depending on the bearing springs a silicon plate up to 3 x 3 mm2 wide and typically 30μm thick can be driven resonantly to rotational or translational movement. Combined with additional optical components and appropriate detectors handheld Czerny-Turner or Fourier Transform spectrometers have been realized and tested. Results of first measurements of reflection spectroscopy on model substances have been performed with both system types in the NIR range. Measurements on real objects like tomatoes or apples are intended for a wider wavelength range. Future systems may contain displays and light sources as well as data storage cards or additional interfaces.

Optimal Band Ratio Analysis of WORLDVIEW-3 Imagery for Bathymetry of Shallow Rivers (case Study: Sarca River, Italy)

NASA Astrophysics Data System (ADS)

Niroumand-Jadidi, M.; Vitti, A.

2016-06-01

The Optimal Band Ratio Analysis (OBRA) could be considered as an efficient technique for bathymetry from optical imagery due to its robustness on substrate variability. This point receives more attention for very shallow rivers where different substrate types can contribute remarkably into total at-sensor radiance. The OBRA examines the total possible pairs of spectral bands in order to identify the optimal two-band ratio that its log transformation yields a strong linear relation with field measured water depths. This paper aims at investigating the effectiveness of additional spectral bands of newly launched WorldView-3 (WV-3) imagery in the visible and NIR spectrum through OBRA for retrieving water depths in shallow rivers. In this regard, the OBRA is performed on a WV-3 image as well as a GeoEye image of a small Alpine river in Italy. In-situ depths are gathered in two river reaches using a precise GPS device. In each testing scenario, 50% of the field data is used for calibration of the model and the remained as independent check points for accuracy assessment. In general, the effect of changes in water depth is highly pronounced in longer wavelengths (i.e. NIR) due to high and rapid absorption of light in this spectrum as long as it is not saturated. As the studied river is shallow, NIR portion of the spectrum has not been reduced so much not to reach the riverbed; making use of the observed radiance over this spectral range as denominator has shown a strong correlation through OBRA. More specifically, tightly focused channels of red-edge, NIR-1 and NIR-2 provide a wealth of choices for OBRA rather than a single NIR band of conventional 4-band images (e.g. GeoEye). This advantage of WV-3 images is outstanding as well for choosing the optimal numerator of the ratio model. Coastal-blue and yellow bands of WV-3 are identified as proper numerators while only green band of the GeoEye image contributed to a reliable correlation of image derived values and field measured depths. According to the results, the additional and narrow spectral bands of WV-3 image lead to an average determination coefficient of 67% in two river segments, which is 10% higher than that of obtained from the 4-band GeoEye image. In addition, RMSEs of depth estimations are calculated as 4 cm and 6 cm respectively for WV-3 and GeoEye images, considering the optimal band ratio.

High sensitivity, wide coverage, and high-resolution NIR non-cryogenic spectrograph, WINERED

NASA Astrophysics Data System (ADS)

Ikeda, Yuji; Kobayashi, Naoto; Kondo, Sohei; Otsubo, Shogo; Hamano, Satoshi; Sameshima, Hiroaki; Yoshikawa, Tomoshiro; Fukue, Kei; Nakanishi, Kenshi; Kawanishi, Takafumi; Nakaoka, Tetsuya; Kinoshita, Masaomi; Kitano, Ayaka; Asano, Akira; Takenaka, Keiichi; Watase, Ayaka; Mito, Hiroyuki; Yasui, Chikako; Minami, Atsushi; Izumu, Natsuko; Yamamoto, Ryo; Mizumoto, Misaki; Arasaki, Takayuki; Arai, Akira; Matsunaga, Noriyuki; Kawakita, Hideyo

2016-08-01

Near-infrared (NIR) high-resolution spectroscopy is a fundamental observational method in astronomy. It provides significant information on the kinematics, the magnetic fields, and the chemical abundances, of astronomical objects embedded in or behind the highly extinctive clouds or at the cosmological distances. Scientific requirements have accelerated the development of the technology required for NIR high resolution spectrographs using 10 m telescopes. WINERED is a near-infrared (NIR) high-resolution spectrograph that is currently mounted on the 1.3 m Araki telescope of the Koyama Astronomical Observatory in Kyoto-Sangyo University, Japan, and has been successfully operated for three years. It covers a wide wavelength range from 0.90 to 1.35 μm (the z-, Y-, and J-bands) with a spectral resolution of R = 28,000 (Wide-mode) and R = 80,000 (Hires-Y and Hires-J modes). WINERED has three distinctive features: (i) optics with no cold stop, (ii) wide spectral coverage, and (iii) high sensitivity. The first feature, originating from the Joyce proposal, was first achieved by WINERED, with a short cutoff infrared array, cold baffles, and custom-made thermal blocking filters, and resulted in reducing the time for development, alignment, and maintenance, as well as the total cost. The second feature is realized with the spectral coverage of Δλ/λ 1/6 in a single exposure. This wide coverage is realized by a combination of a decent optical design with a cross-dispersed echelle and a large format array (2k x 2k HAWAII- 2RG). The Third feature, high sensitivity, is achieved via the high-throughput optics (>60 %) and the very low noise of the system. The major factors affecting the high throughput are the echelle grating and the VPH cross-disperser with high diffraction efficiencies of 83 % and 86 %, respectively, and the high QE of HAWAII-2RG (83 % at 1.23 μm). The readout noise of the electronics and the ambient thermal background radiation at longer wavelengths could be major noise sources. The readout noise is 5.3 e- for NDR = 32, and the ambient thermal background is significantly reduced to 0.05 e- pix-1 sec-1 at 273 K. As a result, the limiting magnitudes of WINERED are estimated to be mJ = 13.8 mag for the 1.3 m telescope, mJ = 16.9 mag for the 3.6 m telescope, and mJ = 19.2 mag for 10 m telescope with adoptive optics, respectively. Finally, we introduce some scientific highlights provided by WINERED for both emission and absorption line objects in the fields of stars, the interstellar medium, and the solar system.

Physics-based subsurface visualization of human tissue.

PubMed

Sharp, Richard; Adams, Jacob; Machiraju, Raghu; Lee, Robert; Crane, Robert

2007-01-01

In this paper, we present a framework for simulating light transport in three-dimensional tissue with inhomogeneous scattering properties. Our approach employs a computational model to simulate light scattering in tissue through the finite element solution of the diffusion equation. Although our model handles both visible and nonvisible wavelengths, we especially focus on the interaction of near infrared (NIR) light with tissue. Since most human tissue is permeable to NIR light, tools to noninvasively image tumors, blood vasculature, and monitor blood oxygenation levels are being constructed. We apply this model to a numerical phantom to visually reproduce the images generated by these real-world tools. Therefore, in addition to enabling inverse design of detector instruments, our computational tools produce physically-accurate visualizations of subsurface structures.

Thermal ablation therapeutics based on CNx multi-walled nanotubes

PubMed Central

Torti, Suzy V; Byrne, Fiona; Whelan, Orla; Levi, Nicole; Ucer, Burak; Schmid, Michael; Torti, Frank M; Akman, Steven; Liu, Jiwen; Ajayan, Pulickel M; Nalamasu, Omkaram; Carroll, David L

2007-01-01

We demonstrate that nitrogen doped, multi-walled carbon nanotubes (CNx-MWNT) result in photo-ablative destruction of kidney cancer cells when excited by near infrared (NIR) irradiation. Further, we show that effective heat transduction and cellular cytotoxicity depends on nanotube length: effective NIR coupling occurs at nanotube lengths that exceed half the wavelength of the stimulating radiation, as predicted in classical antenna theory. We also demonstrate that this radiation heats the nanotubes through induction processes, resulting in significant heat transfer to surrounding media and cell killing at extraordinarily small radiation doses. This cell death was attributed directly to photothermal effect generated within the culture, since neither the infrared irradiation itself nor the CNx-MWNT were toxic to the cells. PMID:18203437

Development of Thermally Stable and Highly Fluorescent IR Dyes

NASA Technical Reports Server (NTRS)

Bu, Xiu R.

2004-01-01

Fluorophores are the core component in various optical applications such as sensors and probes. Fluorphores with low-energy or long wavelength emission, in particular, in NIR region, possess advantages of low interference and high sensitivity. In this study, we has explored several classes of imidazole-based compounds for NIR fluorescent properties and concluded: (1) thiazole-based imidazole compounds are fluorescent; (2) emission energy is tunable by additional donor groups; (3) they also possess impressive two- photon absorption properties; and (4) fluorescence emission can be induced by two- photon input. This report summarizes (1) synthesis of new series of fluorophore; (2) impact of electron-withdrawing groups on fluorescent property; (3) unique property of two-photon absorption; and (4) on-going development.

Photothermal effect of gold nanostars inkjet-printed on coated paper substrate under near-infrared irradiation

NASA Astrophysics Data System (ADS)

Borzenkov, Mykola; Chirico, Giuseppe; Collini, Maddalena; Määttänen, Anni; Ihalainen, Petri; Cabrini, Elisa; Dacarro, Giacomo; Pallavicini, Piersandro

2016-04-01

The research and development of personalized medical treatments is increasing steadily fostered by its large societal impact. The ability of non-spherical gold nanoparticles to locally and efficiently release heat when irradiated in Near Infrared (NIR) wavelength region is a promising tool for photothermal medical therapies. In the present work, stable inks containing PEGylated gold nanostars (GNS) were obtained and inkjet-printed on a pigment coated paper substrate. Significant photothermal effect of the printed patterns was observed under Near Infrared (NIR) excitation of the Localized Surface Plasmon Resonance (LSPR) of the GNS. These preliminary results support, in perspective, the application of printed GNS patterns for thermal medical treatments either by direct localized heating, or by temperature triggered drug release.

Analysis of absorption and spreading of moisturizer on the microscopic region of the skin surface with near-infrared imaging.

PubMed

Arimoto, H; Yanai, M; Egawa, M

2016-11-01

Near-infrared (NIR) light with high water absorption enables us to visualize the water content distribution appeared in the superficial skin layer. The light penetration depth with the wavelength of 1920 nm is almost 100 μm from the skin surface. Thus, the water distribution in the stratum corneum can be effectively imaged by detecting the wavelength band around 1920 nm. The aim of this article was to measure the time-lapse behavior of the tiny droplet of the moisturizer spreading on the skin surface by imaging in 1920 nm wavelength band for investigating the correlation with the traditional index of the skin condition such as the water content and transepidermal water loss (TEWL). Experiment is performed with three moisturizer products and seven volunteer subjects. The NIR image is acquired by an originally designed imaging scope equipped with the white light of the strong brightness [super continuum (SC) light], the bandpass filter with the center wavelength of 1920 nm, and the NIR image sensor. A tiny droplet of the moisturizer is put on the surface of the skin and the time-lapse images are saved. Each acquired image is analyzed from a view point of the droplet area and elapsed time for absorption into the skin. The water content and TEWL of all subjects are measured by the conventional electrical method for investigating the relationship with the measured droplet dynamics parameters. Elapsed time for moisturizer droplet to be absorbed into the skin, the droplet area just before absorption for three moisturizer products, skin water contents, and TEWL for seven subjects were measured and correlation coefficients for each parameters were calculated. It was found that the skin with higher water contents or lower TEWL absorbed the moisturizer faster and spreads moisturizer wider. Also absorption and spreading speed depend on moisturizer property (moisturizing or fresh) which is originated from the moisturizer constituents. The correlation values between the moisturizer dynamics on the skin surface and the traditional index of the skin property were clarified. It was found that the skin with the high water content or low TEWL absorbs the moisturizer droplet fast. The spreading area depends not only on the skin property but on the constituents of the moisturizers. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Double NIR laser stimulation and enhancing the thermal sensitivity of Er3+/Tm3+/Nd3+ doped multilayer core-shell nanoparticles.

PubMed

Ba, Zhaojing; Hu, Min; Zhao, Yiming; Wang, Yiqing; Wang, Jing; Zhang, Zhenxi

2018-08-31

Non-contact thermal sensors are important devices to study cellular processes and monitor temperature in vivo. Herein, a novel highly sensitive nanothermometer based on NaYF 4 :Yb,Er@ NaYF 4 @NaYF 4 :Yb,Tm@ NaYF 4 :Nd (denoted as Er@Y@Tm@Nd) was prepared by a facile solvothermal method. When excited by the near-infrared (NIR) light of 808 and 980 nm, the as-prepared Er@Y@Tm@Nd nanoparticles could emit both blue and green light, respectively, since the lanthanide cations responsible for these emissions are gathered inside this nanostructure. The green and blue light intensity ratio exhibits obvious temperature dependence in the range of the physiological temperature. Additionally, the fluorescence intensity of Er 3+ and Tm 3+ are also greatly enhanced due to the multilayer structure that implies avoiding the Er 3+ and Tm 3+ energy cross-relaxation by introduction of a NaYF 4 wall between them. The as-prepared core-shell-shell-shell structure with Er 3+ and Tm 3+ in different layers improves dozens of times of the thermal sensitivity based on the non-thermal coupling levels of the probe: the maximum values for the sensitivity are 2.95% K -1 (I Er-521 /I Tm-450 ) and 6.30% K -1 (I Tm-474 /I Er-541 ) when excited by 980 and 808 nm laser sources, respectively. These values are well above those previously reported (<0.7% K -1 ), indicating that the prepared nanostructures are temperature sensors with excellent thermal sensitivity and sensitive to NIR wavelength excitation that makes them highly preferred for thermal detection.

Sh2-138: physical environment around a small cluster of massive stars

NASA Astrophysics Data System (ADS)

Baug, T.; Ojha, D. K.; Dewangan, L. K.; Ninan, J. P.; Bhatt, B. C.; Ghosh, S. K.; Mallick, K. K.

2015-12-01

We present a multiwavelength study of the Sh2-138, a Galactic compact H II region. The data comprise of optical and near-infrared (NIR) photometric and spectroscopic observations from the 2-m Himalayan Chandra Telescope, radio observations from the Giant Metrewave Radio Telescope (GMRT), and archival data covering radio through NIR wavelengths. A total of 10 Class I and 54 Class II young stellar objects (YSOs) are identified in a 4.6 arcmin×4.6 arcmin area of the Sh2-138 region. Five compact ionized clumps, with four lacking of any optical or NIR counterparts, are identified using the 1280 MHz radio map, and correspond to sources with spectral type earlier than B0.5. Free-free emission spectral energy distribution fitting of the central compact H II region yields an electron density of ˜2250 ± 400 cm-3. With the aid of a wide range of spectra, from 0.5-15 μm, the central brightest source - previously hypothesized to be the main ionizing source - is characterized as a Herbig Be type star. At large scale (15 arcmin ×15 arcmin), the Herschel images (70-500 μm) and the nearest neighbour analysis of YSOs suggest the formation of an isolated cluster at the junction of filaments. Furthermore, using a greybody fit to the dust spectrum, the cluster is found to be associated with the highest column density (˜3 × 1022 cm-2) and high temperature (˜35 K) regime, as well as with the radio continuum emission. The mass of the central clump seen in the column density map is estimated to be ˜3770 M⊙.

Optical biopsy of the prostate: can we TRUST (trans-rectal ultrasound-coupled spectral tomography)?

NASA Astrophysics Data System (ADS)

Piao, Daqing; Jiang, Zhen; Bartels, Kenneth E.; Holyoak, G. Reed; Ritchey, Jerry W.; Rock, Kendra; Ownby, Charlotte L.; Bunting, Charles F.; Slobodov, Gennady

2011-03-01

Needle-based core-biopsy to locate prostate cancer relies heavily upon trans-rectal ultrasound (TRUS) imaging guidance. Ultrasonographic findings of classic hypoechoic peripheral zone lesions have a low specificity of ~28%, a low positive predictive value of ~29%, and an overall accuracy of ~43%, in prostate cancer diagnosis. The prevalence of isoechoic or nearly invisible prostate cancers on ultrasonography ranges from 25 to 42%. As a result, TRUS is useful and convenient to direct the needle trajectory following a systematic biopsy sampling template rather than to target only the potentially malignant lesion for focal-biopsy. To address this deficiency in the first-line of prostate cancer imaging, a trans-rectal ultrasound-coupled spectral tomography (TRUST) approach is being developed to non-invasively resolve the likely optical signatures of prostate malignancy. The approach has evolved from using one NIR wavelength to two NIR bands, and recently to three bands of NIR spectrum information. The concept has been evaluated on one normal canine prostate and three dogs with implanted prostate tumor developed as a model. The initial results implementing TRUST on the canine prostate tumor model includes: (1) quantifying substantially increased total hemoglobin concentration over the time-course of imaging in a rapidly growing prostate tumor; (2) confirming hypoxia in a prostatic cystic lesion; and (3) imaging hypoxic changes of a necrotic prostate tumor. Despite these interesting results, intensive technologic development is necessary for translating the approach to benefiting clinical practice, wherein the ultimate utility is not possibly to eliminate needle-biopsy but to perform focal-biopsy that is only necessary to confirm the cancer, as well as to monitor and predict treatment responses.

On the Chemical Abundances of Miras in Clusters: V1 in the Metal-rich Globular NGC 5927

NASA Astrophysics Data System (ADS)

D’Orazi, V.; Magurno, D.; Bono, G.; Matsunaga, N.; Braga, V. F.; Elgueta, S. S.; Fukue, K.; Hamano, S.; Inno, L.; Kobayashi, N.; Kondo, S.; Monelli, M.; Nonino, M.; Przybilla, N.; Sameshima, H.; Saviane, I.; Taniguchi, D.; Thevenin, F.; Urbaneja-Perez, M.; Watase, A.; Arai, A.; Bergemann, M.; Buonanno, R.; Dall’Ora, M.; Da Silva, R.; Fabrizio, M.; Ferraro, I.; Fiorentino, G.; Francois, P.; Gilmozzi, R.; Iannicola, G.; Ikeda, Y.; Jian, M.; Kawakita, H.; Kudritzki, R. P.; Lemasle, B.; Marengo, M.; Marinoni, S.; Martínez-Vázquez, C. E.; Minniti, D.; Neeley, J.; Otsubo, S.; Prieto, J. L.; Proxauf, B.; Romaniello, M.; Sanna, N.; Sneden, C.; Takenaka, K.; Tsujimoto, T.; Valenti, E.; Yasui, C.; Yoshikawa, T.; Zoccali, M.

2018-03-01

We present the first spectroscopic abundance determination of iron, α-elements (Si, Ca, and Ti), and sodium for the Mira variable V1 in the metal-rich globular cluster NGC 5927. We use high-resolution (R ∼ 28,000), high signal-to-noise ratio (∼200) spectra collected with WINERED, a near-infrared (NIR) spectrograph covering simultaneously the wavelength range 0.91–1.35 μm. The effective temperature and the surface gravity at the pulsation phase of the spectroscopic observation were estimated using both optical (V) and NIR time-series photometric data. We found that the Mira is metal-rich ([Fe/H] = ‑0.55 ± 0.15) and moderately α-enhanced ([α/Fe] = 0.15 ± 0.01, σ = 0.2). These values agree quite well with the mean cluster abundances based on high-resolution optical spectra of several cluster red giants available in the literature ([Fe/H] = ‑ 0.47 ± 0.06, [α/Fe] = + 0.24 ± 0.05). We also found a Na abundance of +0.35 ± 0.20 that is higher than the mean cluster abundance based on optical spectra (+0.18 ± 0.13). However, the lack of similar spectra for cluster red giants and that of corrections for departures from local thermodynamical equilibrium prevents us from establishing whether the difference is intrinsic or connected with multiple populations. These findings indicate a strong similarity between optical and NIR metallicity scales in spite of the difference in the experimental equipment, data analysis, and in the adopted spectroscopic diagnostics. Based on spectra collected with the WINERED spectrograph available as a visitor instrument at the ESO New Technology Telescope (NTT), La Silla, Chile (ESO Proposal: 098.D-0878(A), PI: G. Bono).

Detection of Rotational Spectral Variation on the M-type Asteroid (16) Psyche

NASA Astrophysics Data System (ADS)

Sanchez, Juan A.; Reddy, Vishnu; Shepard, Michael K.; Thomas, Cristina; Cloutis, Edward A.; Takir, Driss; Conrad, Albert; Kiddell, Cain; Applin, Daniel

2017-01-01

The asteroid (16) Psyche is of scientific interest because it contains ˜1% of the total mass of the asteroid belt and is thought to be the remnant metallic core of a protoplanet. Radar observations have indicated the significant presence of metal on the surface with a small percentage of silicates. Prior ground-based observations showed rotational variations in the near-infrared (NIR) spectra and radar albedo of this asteroid. However, no comprehensive study that combines multi-wavelength data has been conducted so far. Here we present rotationally resolved NIR spectra (0.7-2.5 μm) of (16) Psyche obtained with the NASA Infrared Telescope Facility. These data have been combined with shape models of the asteroid for each rotation phase. Spectral band parameters extracted from the NIR spectra show that the pyroxene band center varies from ˜0.92 to 0.94 μm. Band center values were used to calculate the pyroxene chemistry of the asteroid, whose average value was found to be Fs30En65Wo5. Variations in the band depth (BD) were also observed, with values ranging from 1.0% to 1.5%. Using a new laboratory spectral calibration method, we estimated an average orthopyroxene content of 6% ± 1%. The mass-deficit region of Psyche, which exhibits the highest radar albedo, also shows the highest value for the spectral slope and the minimum BD. The spectral characteristics of Psyche suggest that its parent body did not have the typical structure expected for a differentiated body or that the sequence of events that led to its current state was more complex than previously thought.

DETECTION OF ROTATIONAL SPECTRAL VARIATION ON THE M-TYPE ASTEROID (16) PSYCHE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanchez, Juan A.; Thomas, Cristina; Reddy, Vishnu

The asteroid (16) Psyche is of scientific interest because it contains ∼1% of the total mass of the asteroid belt and is thought to be the remnant metallic core of a protoplanet. Radar observations have indicated the significant presence of metal on the surface with a small percentage of silicates. Prior ground-based observations showed rotational variations in the near-infrared (NIR) spectra and radar albedo of this asteroid. However, no comprehensive study that combines multi-wavelength data has been conducted so far. Here we present rotationally resolved NIR spectra (0.7–2.5 μ m) of (16) Psyche obtained with the NASA Infrared Telescope Facility.more » These data have been combined with shape models of the asteroid for each rotation phase. Spectral band parameters extracted from the NIR spectra show that the pyroxene band center varies from ∼0.92 to 0.94 μ m. Band center values were used to calculate the pyroxene chemistry of the asteroid, whose average value was found to be Fs{sub 30}En{sub 65}Wo{sub 5}. Variations in the band depth (BD) were also observed, with values ranging from 1.0% to 1.5%. Using a new laboratory spectral calibration method, we estimated an average orthopyroxene content of 6% ± 1%. The mass-deficit region of Psyche, which exhibits the highest radar albedo, also shows the highest value for the spectral slope and the minimum BD. The spectral characteristics of Psyche suggest that its parent body did not have the typical structure expected for a differentiated body or that the sequence of events that led to its current state was more complex than previously thought.« less

Piezo-Phototronic Effect on Selective Electron or Hole Transport through Depletion Region of Vis-NIR Broadband Photodiode.

PubMed

Zou, Haiyang; Li, Xiaogan; Peng, Wenbo; Wu, Wenzhuo; Yu, Ruomeng; Wu, Changsheng; Ding, Wenbo; Hu, Fei; Liu, Ruiyuan; Zi, Yunlong; Wang, Zhong Lin

2017-08-01

Silicon underpins nearly all microelectronics today and will continue to do so for some decades to come. However, for silicon photonics, the indirect band gap of silicon and lack of adjustability severely limit its use in applications such as broadband photodiodes. Here, a high-performance p-Si/n-ZnO broadband photodiode working in a wide wavelength range from visible to near-infrared light with high sensitivity, fast response, and good stability is reported. The absorption of near-infrared wavelength light is significantly enhanced due to the nanostructured/textured top surface. The general performance of the broadband photodiodes can be further improved by the piezo-phototronic effect. The enhancement of responsivity can reach a maximum of 78% to 442 nm illumination, the linearity and saturation limit to 1060 nm light are also significantly increased by applying external strains. The photodiode is illuminated with different wavelength lights to selectively choose the photogenerated charge carriers (either electrons or holes) passing through the depletion region, to investigate the piezo-phototronic effect on electron or hole transport separately for the first time. This is essential for studying the basic principles in order to develop a full understanding about piezotronics and it also enables the development of the better performance of optoelectronics. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Transcranial low-level laser therapy improves brain mitochondrial function and cognitive impairment in D-galactose-induced aging mice.

PubMed

Salehpour, Farzad; Ahmadian, Nahid; Rasta, Seyed Hossein; Farhoudi, Mehdi; Karimi, Pouran; Sadigh-Eteghad, Saeed

2017-10-01

Mitochondrial function plays a key role in the aging-related cognitive impairment, and photoneuromodulation of mitochondria by transcranial low-level laser therapy (LLLT) may contribute to its improvement. This study focused on the transcranial LLLT effects on the D-galactose (DG)-induced mitochondrial dysfunction, apoptosis, and cognitive impairment in mice. For this purpose, red and near-infrared (NIR) laser wavelengths (660 and 810 nm) at 2 different fluencies (4 and 8 J/cm 2 ) at 10-Hz pulsed wave mode were administrated transcranially 3 d/wk in DG-received (500 mg/kg/subcutaneous) mice model of aging for 6 weeks. Spatial and episodic-like memories were assessed by the Barnes maze and What-Where-Which (WWWhich) tasks. Brain tissues were analyzed for mitochondrial function including active mitochondria, adenosine triphosphate, and reactive oxygen species levels, as well as membrane potential and cytochrome c oxidase activity. Apoptosis-related biomarkers, namely, Bax, Bcl-2, and caspase-3 were evaluated by Western blotting method. Laser treatments at wavelengths of 660 and 810 nm at 8 J/cm 2 attenuated DG-impaired spatial and episodic-like memories. Also, results showed an obvious improvement in the mitochondrial function aspects and modulatory effects on apoptotic markers in aged mice. However, same wavelengths at the fluency of 4 J/cm 2 had poor effect on the behavioral and molecular indexes in aging model. This data indicates that transcranial LLLT at both of red and NIR wavelengths at the fluency of 8 J/cm 2 has a potential to ameliorate aging-induced mitochondrial dysfunction, apoptosis, and cognitive impairment. Copyright © 2017 Elsevier Inc. All rights reserved.

Assessment of beer quality based on foamability and chemical composition using computer vision algorithms, near infrared spectroscopy and machine learning algorithms.

PubMed

Gonzalez Viejo, Claudia; Fuentes, Sigfredo; Torrico, Damir; Howell, Kate; Dunshea, Frank R

2018-01-01

Beer quality is mainly defined by its colour, foamability and foam stability, which are influenced by the chemical composition of the product such as proteins, carbohydrates, pH and alcohol. Traditional methods to assess specific chemical compounds are usually time-consuming and costly. This study used rapid methods to evaluate 15 foam and colour-related parameters using a robotic pourer (RoboBEER) and chemical fingerprinting using near infrared spectroscopy (NIR) from six replicates of 21 beers from three types of fermentation. Results from NIR were used to create partial least squares regression (PLS) and artificial neural networks (ANN) models to predict four chemometrics such as pH, alcohol, Brix and maximum volume of foam. The ANN method was able to create more accurate models (R 2  = 0.95) compared to PLS. Principal components analysis using RoboBEER parameters and NIR overtones related to protein explained 67% of total data variability. Additionally, a sub-space discriminant model using the absorbance values from NIR wavelengths resulted in the successful classification of 85% of beers according to fermentation type. The method proposed showed to be a rapid system based on NIR spectroscopy and RoboBEER outputs of foamability that can be used to infer the quality, production method and chemical parameters of beer with minimal laboratory equipment. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Reducing motion artifacts for long-term clinical NIRS monitoring using collodion-fixed prism-based optical fibers

PubMed Central

Yücel, Meryem A.; Selb, Juliette; Boas, David A.; Cash, Sydney S.; Cooper, Robert J.

2013-01-01

As the applications of near-infrared spectroscopy (NIRS) continue to broaden and long-term clinical monitoring becomes more common, minimizing signal artifacts due to patient movement becomes more pressing. This is particularly true in applications where clinically and physiologically interesting events are intrinsically linked to patient movement, as is the case in the study of epileptic seizures. In this study, we apply an approach common in the application of EEG electrodes to the application of specialized NIRS optical fibers. The method provides improved optode-scalp coupling through the use of miniaturized optical fiber tips fixed to the scalp using collodion, a clinical adhesive. We investigate and quantify the performance of this new method in minimizing motion artifacts in healthy subjects, and apply the technique to allow continuous NIRS monitoring throughout epileptic seizures in two epileptic in-patients. Using collodion-fixed fibers reduces the percent signal change of motion artifacts by 90 % and increases the SNR by 6 and 3 fold at 690 and 830 nm wavelengths respectively when compared to a standard Velcro-based array of optical fibers. The change in both HbO and HbR during motion artifacts is found to be statistically lower for the collodion-fixed fiber probe. The collodion-fixed optical fiber approach has also allowed us to obtain good quality NIRS recording of three epileptic seizures in two patients despite excessive motion in each case. PMID:23796546

Study of visible luminescence spectra from Nd3+ doped TPO glass upon 808 nm excitation

NASA Astrophysics Data System (ADS)

Azam, Mohd; Rai, Vineet Kumar

2018-05-01

The Nd3+ doped TPO glasses have been prepared by melting and quenching method using the high temperature electric furnace. The upconversion (UC) spectra of Nd3+ doped TPO glasses at different concentration of rare ions have been recorded in the 400-700 nm wavelength range upon 808 nm laser excitation source. In the UC emission process, there are four bands centered at ˜495 nm, 546 nm, 602 nm and 653 nm respectively in the visible range were observed. But the green and red bands centered at˜546 nm and ˜653 nm corresponding to the 4G7/2 → 4I9/2 and 4G7/2 → 4I13/2 transitions respectively have been observed as the strong bands. The UC emission mechanism was observed as two photon process. The material can be used as a good NIR to visible upconverter and non-colour tunable display.

Multiple resonant absorber with prism-incorporated graphene and one-dimensional photonic crystals in the visible and near-infrared spectral range

NASA Astrophysics Data System (ADS)

Zou, X. J.; Zheng, G. G.; Chen, Y. Y.; Xu, L. H.; Lai, M.

2018-04-01

A multi-band absorber constructed from prism-incorporated one-dimensional photonic crystal (1D-PhC) containing graphene defects is achieved theoretically in the visible and near-infrared (vis-NIR) spectral range. By means of the transfer matrix method (TMM), the effect of structural parameters on the optical response of the structure has been investigated. It is possible to achieve multi-peak and complete optical absorption. The simulations reveal that the light intensity is enhanced at the graphene plane, and the resonant wavelength and the absorption intensity can also be tuned by tilting the incidence angle of the impinging light. In particular, multiple graphene sheets are embedded in the arrays, without any demand of manufacture process to cut them into periodic patterns. The proposed concept can be extended to other two-dimensional (2D) materials and engineered for promising applications, including selective or multiplex filters, multiple channel sensors, and photodetectors.

Hyperspectral imaging in SWIR: from stain-free microscopy to deep tissue imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Berezin, Mikhail Y.

2016-03-01

Recent advances in relatively unexplored short wave infrared (SWIR) range from 800-1600 nm detectors make wide-field imaging in this spectral range attractive to biology. The distinct advantages of SWIR region over the visible and near infrared (NIR) in tissue analysis are two-fold: (i) high abundance endogenous chromophores (i.e. water and lipids) enable tissue component differentiation based on wavelength-dependent absorption properties and (ii) the weak scattering of tissue permits better resolution of imaging in thick specimens. When combined with high spectral resolution, SWIR imaging produces a spectroscopic image, where every pixel corresponds to the entire high-resolution spectrum. This hyperspectral (HS) approach provides rich information about the relative abundance of individual chromophores and their interactions that contribute to the intensity and location of the optical signal. The presentation discusses the challenges in the SWIR-HS instrument design and data analysis and demonstrates some of the promising applications of this technology in life science and medicine.

Spectrophotometry of Wolf-Rayet stars. I - Continuum energy distributions

NASA Technical Reports Server (NTRS)

Morris, Patrick W.; Brownsberger, Kenneth R.; Conti, Peter S.; Massey, Philip; Vacca, William D.

1993-01-01

All available low-resolution IUE spectra are assembled for Galactic, LMC, and SMC W-R stars and are merged with ground-based optical and NIR spectra in order to collate in a systematic fashion the shapes of these energy distributions over the wavelength range 0.1-1 micron. They can be consistently fitted by a power law of the form F(lambda) is approximately equal to lambda exp -alpha over the range 1500-9000 A to derive color excesses E(B-V) and spectral indices by removing the 2175-A interstellar absorption feature. The WN star color excesses derived are found to be in good agreement with those of Schmutz and Vacca (1991) and Koesterke et al. (1991). Significant heterogeneity in spectral index values was generally seen with any given subtype, but the groups consisting of the combined set of Galactic and LMC W-R stars, the separate WN and WC sequences, and the Galactic and LMC W-R stars all showed a striking and consistent Gaussian-like frequency distribution of values.

Subcutaneous Photovoltaic Infrared Energy Harvesting for Bio-Implantable Devices.

PubMed

Moon, Eunseong; Blaauw, David; Phillips, Jamie D

2017-05-01

Wireless biomedical implantable devices on the mm-scale enable a wide range of applications for human health, safety, and identification, though energy harvesting and power generation are still looming challenges that impede their widespread application. Energy scavenging approaches to power biomedical implants have included thermal [1-3], kinetic [4-6], radio-frequency [7-11] and radiative sources [12-14]. However, the achievement of efficient energy scavenging for biomedical implants at the mm-scale has been elusive. Here we show that photovoltaic cells at the mm-scale can achieve a power conversion efficiency of more than 17 % for silicon and 31 % for GaAs under 1.06 μW/mm 2 infrared irradiation at 850 nm. Finally, these photovoltaic cells demonstrate highly efficient energy harvesting through biological tissue from ambient sunlight, or irradiation from infrared sources such as used in present-day surveillance systems, by utilizing the near infrared (NIR) transparency window between the 650 nm and 950 nm wavelength range [15-17].

Subcutaneous Photovoltaic Infrared Energy Harvesting for Bio-Implantable Devices

PubMed Central

Moon, Eunseong; Blaauw, David; Phillips, Jamie D.

2017-01-01

Wireless biomedical implantable devices on the mm-scale enable a wide range of applications for human health, safety, and identification, though energy harvesting and power generation are still looming challenges that impede their widespread application. Energy scavenging approaches to power biomedical implants have included thermal [1–3], kinetic [4–6], radio-frequency [7–11] and radiative sources [12–14]. However, the achievement of efficient energy scavenging for biomedical implants at the mm-scale has been elusive. Here we show that photovoltaic cells at the mm-scale can achieve a power conversion efficiency of more than 17 % for silicon and 31 % for GaAs under 1.06 μW/mm2 infrared irradiation at 850 nm. Finally, these photovoltaic cells demonstrate highly efficient energy harvesting through biological tissue from ambient sunlight, or irradiation from infrared sources such as used in present-day surveillance systems, by utilizing the near infrared (NIR) transparency window between the 650 nm and 950 nm wavelength range [15–17]. PMID:29056754

Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development

PubMed Central

Moran, Zelda R.; Parker, Andrew G.

2016-01-01

Abstract Near infrared (NIR) photography and video was investigated as a method for observing and recording intrapuparial development in the tsetse fly Glossina palpalis gambiensis and other Muscomorpha (Cyclorrhapha) Diptera. We showed that NIR light passes through the puparium, permitting images of the true pupae and pharate adult to be captured. Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs. This study was carried out to advance our understanding of tsetse pupal development, specifically with the goal of improving a sorting technique which could separate male from female tsetse flies several days before emergence. Separation of the sexes at this stage is highly desirable for operational tsetse sterile insect technique control programmes, as it would permit the easy retention of females for the colony while allowing the males to be handled, irradiated and shipped in the pupal stage when they are less sensitive to vibration. In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications. NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption. PMID:27402791

A review on the applications of portable near-infrared spectrometers in the agro-food industry.

PubMed

dos Santos, Cláudia A Teixeira; Lopo, Miguel; Páscoa, Ricardo N M J; Lopes, João A

2013-11-01

Industry has created the need for a cost-effective and nondestructive quality-control analysis system. This requirement has increased interest in near-infrared (NIR) spectroscopy, leading to the development and marketing of handheld devices that enable new applications that can be implemented in situ. Portable NIR spectrometers are powerful instruments offering several advantages for nondestructive, online, or in situ analysis: small size, low cost, robustness, simplicity of analysis, sample user interface, portability, and ergonomic design. Several studies of on-site NIR applications are presented: characterization of internal and external parameters of fruits and vegetables; conservation state and fat content of meat and fish; distinguishing among and quality evaluation of beverages and dairy products; protein content of cereals; evaluation of grape ripeness in vineyards; and soil analysis. Chemometrics is an essential part of NIR spectroscopy manipulation because wavelength-dependent scattering effects, instrumental noise, ambient effects, and other sources of variability may complicate the spectra. As a consequence, it is difficult to assign specific absorption bands to specific functional groups. To achieve useful and meaningful results, multivariate statistical techniques (essentially involving regression techniques coupled with spectral preprocessing) are therefore required to extract the information hidden in the spectra. This work reviews the evolution of the use of portable near-infrared spectrometers in the agro-food industry.

Optimization measurement of muscle oxygen saturation under isometric studies using FNIRS

NASA Astrophysics Data System (ADS)

Halim, A. A. A.; Laili, M. H.; Salikin, M. S.; Rusop, M.

2018-05-01

Development of functional near infrared spectroscopy (fNIRS) technologies has advanced quantification signal using multiple wavelength and detector to investigate hemodynamic response in human muscle. These non-invasive technologies have been widely used to solve the propagation of light inside the tissues including the absorption, scattering coefficient and to quantify the oxygenation level of haemoglobin and myoglobin in human muscle. The goal of this paper is to optimize the measurement of muscle oxygen saturation during isometric exercise using functional near infrared spectroscopy (fNIRS). The experiment was carried out on 15 sedentary healthy male volunteers. All volunteers are required to perform an isometric exercise at three assessment of muscular fatigue's level on flexor digitalis (FDS) muscle in the human forearm using fNIRS. The slopes of the signals have been highlighted to evaluate the muscle oxygen saturation of regional muscle fatigue. As a result, oxygen saturation slope from 10% exercise showed steeper than the first assessment at 30%-50% of fatigues level. The hemodynamic signal response showed significant value (p=0.04) at all three assessment of muscular fatigue's level which produce a p-value (p<0.05) measured by fNIRS. Thus, this highlighted parameter could be used to estimate fatigue's level of human and could open other possibilities to study muscle performance diagnosis.

Clinical application of photodynamic medicine technology using light-emitting fluorescence imaging based on a specialized luminous source.

PubMed

Namikawa, Tsutomu; Fujisawa, Kazune; Munekage, Eri; Iwabu, Jun; Uemura, Sunao; Tsujii, Shigehiro; Maeda, Hiromichi; Kitagawa, Hiroyuki; Fukuhara, Hideo; Inoue, Keiji; Sato, Takayuki; Kobayashi, Michiya; Hanazaki, Kazuhiro

2018-04-04

The natural amino acid 5-aminolevulinic acid (ALA) is a protoporphyrin IX (PpIX) precursor and a new-generation photosensitive substance that accumulates specifically in cancer cells. When indocyanine green (ICG) is irradiated with near-infrared (NIR) light, it shifts to a higher energy state and emits infrared light with a longer wavelength than the irradiated NIR light. Photodynamic diagnosis (PDD) using ALA and ICG-based NIR fluorescence imaging has emerged as a new diagnostic technique. Specifically, in laparoscopic examinations for serosa-invading advanced gastric cancer, peritoneal metastases could be detected by ALA-PDD, but not by conventional visible-light imaging. The HyperEye Medical System (HEMS) can visualize ICG fluorescence as color images simultaneously projected with visible light in real time. This ICG fluorescence method is widely applicable, including for intraoperative identification of sentinel lymph nodes, visualization of blood vessels in organ resection, and blood flow evaluation during surgery. Fluorescence navigation by ALA-PDD and NIR using ICG imaging provides good visualization and detection of the target lesions that is not possible with the naked eye. We propose that this technique should be used in fundamental research on the relationship among cellular dynamics, metabolic enzymes, and tumor tissues, and to evaluate clinical efficacy and safety in multicenter cooperative clinical trials.

A three-wavelength multi-channel brain functional imager based on digital lock-in photon-counting technique

NASA Astrophysics Data System (ADS)

Ding, Xuemei; Wang, Bingyuan; Liu, Dongyuan; Zhang, Yao; He, Jie; Zhao, Huijuan; Gao, Feng

2018-02-01

During the past two decades there has been a dramatic rise in the use of functional near-infrared spectroscopy (fNIRS) as a neuroimaging technique in cognitive neuroscience research. Diffuse optical tomography (DOT) and optical topography (OT) can be employed as the optical imaging techniques for brain activity investigation. However, most current imagers with analogue detection are limited by sensitivity and dynamic range. Although photon-counting detection can significantly improve detection sensitivity, the intrinsic nature of sequential excitations reduces temporal resolution. To improve temporal resolution, sensitivity and dynamic range, we develop a multi-channel continuous-wave (CW) system for brain functional imaging based on a novel lock-in photon-counting technique. The system consists of 60 Light-emitting device (LED) sources at three wavelengths of 660nm, 780nm and 830nm, which are modulated by current-stabilized square-wave signals at different frequencies, and 12 photomultiplier tubes (PMT) based on lock-in photon-counting technique. This design combines the ultra-high sensitivity of the photon-counting technique with the parallelism of the digital lock-in technique. We can therefore acquire the diffused light intensity for all the source-detector pairs (SD-pairs) in parallel. The performance assessments of the system are conducted using phantom experiments, and demonstrate its excellent measurement linearity, negligible inter-channel crosstalk, strong noise robustness and high temporal resolution.

Characteristics of several NIR tuneable diode lasers for spectroscopic based gas sensing: a comparison.

PubMed

Weldon, Vincent; McInerney, David; Phelan, Richard; Lynch, Michael; Donegan, John

2006-04-01

Tuneable laser diodes were characterized and compared for use as tuneable sources in gas absorption spectroscopy. Specifically, the characteristics of monolithic widely tuneable single frequency lasers, such as sampled grating distributed Bragg reflector laser and modulated grating Y-branch laser diodes, recently developed for optical communications, with operating wavelengths in the 1,520 nm

Quasi-supercontinuum source in the extreme ultraviolet using multiple frequency combs from high-harmonic generation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wünsche, Martin; Fuchs, Silvio; Aull, Stefan

A quasi-supercontinuum source in the extreme ultraviolet (XUV) is demonstrated using a table-top femtosecond laser and a tunable optical parametric amplifier (OPA) as a driver for high-harmonic generation (HHG). The harmonic radiation, which is usually a comb of odd multiples of the fundamental frequency, is generated by near-infrared (NIR) laser pulses from the OPA. A quasi-continuous XUV spectrum in the range of 30 to 100 eV is realized by averaging over multiple harmonic comb spectra with slightly different fundamental frequencies and thus different spectral spacing between the individual harmonics. The driving laser wavelength is swept automatically during an averaging timemore » period. With a total photon flux of 4×10 9 photons/s in the range of 30 eV to 100 eV and 1×10 7 photons/s in the range of 100 eV to 200 eV, the resulting quasi-supercontinuum XUV source is suited for applications such as XUV coherence tomography (XCT) or near-edge absorption fine structure spectroscopy (NEXAFS).« less

Quasi-supercontinuum source in the extreme ultraviolet using multiple frequency combs from high-harmonic generation

DOE PAGES

Wünsche, Martin; Fuchs, Silvio; Aull, Stefan; ...

2017-03-16

A quasi-supercontinuum source in the extreme ultraviolet (XUV) is demonstrated using a table-top femtosecond laser and a tunable optical parametric amplifier (OPA) as a driver for high-harmonic generation (HHG). The harmonic radiation, which is usually a comb of odd multiples of the fundamental frequency, is generated by near-infrared (NIR) laser pulses from the OPA. A quasi-continuous XUV spectrum in the range of 30 to 100 eV is realized by averaging over multiple harmonic comb spectra with slightly different fundamental frequencies and thus different spectral spacing between the individual harmonics. The driving laser wavelength is swept automatically during an averaging timemore » period. With a total photon flux of 4×10 9 photons/s in the range of 30 eV to 100 eV and 1×10 7 photons/s in the range of 100 eV to 200 eV, the resulting quasi-supercontinuum XUV source is suited for applications such as XUV coherence tomography (XCT) or near-edge absorption fine structure spectroscopy (NEXAFS).« less

Dielectric, optical and mechanical studies of phenolic polyene OH1 organic electrooptic crystal

NASA Astrophysics Data System (ADS)

Bharath, D.; Kalainathan, S.

2014-11-01

2-{3-[2-(4-Hydroxyphenyl) vinyl]-5, 5-dimethylcyclo-hex-2-en-1-ylidene}malononitrile (OH1) phenolic locked polyene organic material has been synthesized by the Knoevenagel condensation method. OH1 single crystals were grown in methanol by a slow evaporation method. In order to avoid the multinucleation and reduce the metastable zone width, phosphoric acid is added in different concentrations. The linear optical property of OH1 crystal has been studied using UV-vis-NIR spectroscopy in the wavelength range 190-1100 nm and optical constants are calculated theoretically. The magnitude of nonlinear refractive index (10-12 m2/W), nonlinear absorption (10-6 m/W) and third order nonlinear susceptibility (10-6 esu) has been studied using a Z-scan technique. Dielectric property of OH1 crystal has been studied in frequency range 50 Hz-5 MHz. Photoluminescence spectrum was recorded using a xenon lamp in the range of 450-700 nm. Laser optical damage threshold of OH1 crystal was obtained (0.62 GW/cm2) using a pulsed Nd-YAG laser (1064 nm) of repetition rate 10 ns.

Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT.

PubMed

Paavo, Maarjaliis; Lee, Winston; Merriam, John; Bearelly, Srilaxmi; Tsang, Stephen; Chang, Stanley; Sparrow, Janet R

2017-09-01

We sought to determine whether information revealed from the reflectance, autofluorescence, and absorption properties of RPE cells situated posterior to reticular pseudodrusen (RPD) could provide insight into the origins and structure of RPD. RPD were studied qualitatively by near-infrared fundus autofluorescence (NIR-AF), short-wavelength fundus autofluorescence (SW-AF), and infrared reflectance (IR-R) images, and the presentation was compared to horizontal and en face spectral domain optical coherence tomographic (SD-OCT) images. Images were acquired from 23 patients (39 eyes) diagnosed with RPD (mean age 80.7 ± 7.1 [SD]; 16 female; 4 Hispanics, 19 non-Hispanic whites). In SW-AF, NIR-AF, and IR-R images, fundus RPD were recognized as interlacing networks of small scale variations in IR-R and fluorescence (SW-AF, NIR-AF) intensities. Darkened foci of RPD colocalized in SW-AF and NIR-AF images, and in SD-OCT images corresponded to disturbances of the interdigitation (IZ) and ellipsoid (EZ) zones and to more pronounced hyperreflective lesions traversing photoreceptor-attributable bands in SD-OCT images. Qualitative assessment of the outer nuclear layer (ONL) revealed thinning as RPD extended radially from the outer to inner retina. In en face OCT, hyperreflective areas in the EZ band correlated topographically with hyporeflective foci at the level of the RPE. The hyperreflective lesions corresponding to RPD in SD-OCT scans are likely indicative of degenerating photoreceptor cells. The darkened foci at positions of RPD in NIR-AF and en face OCT images indicate changes in the RPE monolayer with the reduced NIR-AF and en face OCT signal suggesting a reduction in melanin that could be accounted for by RPE thinning.

Photoswitching Near-Infrared Fluorescence from Polymer Nanoparticles Catapults Signals over the Region of Noises and Interferences for Enhanced Sensitivity.

PubMed

Wang, Jie; Lv, Yanlin; Wan, Wei; Wang, Xuefei; Li, Alexander D Q; Tian, Zhiyuan

2016-02-01

As a very sensitive technique, photoswitchable fluorescence not only gains ultrasensitivity but also imparts many novel and unexpected applications. Applications of near-infrared (NIR) fluorescence have demonstrated low background noises, high tissue-penetrating ability, and an ability to reduce photodamage to live cells. Because of these desired features, NIR-fluorescent dyes have been the premium among fluorescent dyes, and probes with photoswitchable NIR fluorescence are even more desirable for enhanced signal quality in the emerging optical imaging modalities but rarely used because they are extremely challenging to design and construct. Using a spiropyran derivative functioning as both a photoswitch and a fluorophore to launch its periodically modulated red fluorescence excitation energy into a NIR acceptor, we fabricated core-shell polymer nanoparticles exhibiting a photoswitchable fluorescence signal within the biological window (∼700-1000 nm) with a peak maximum of 776 nm. Live cells constantly synthesize new molecules, including fluorescent molecules, and also endocytose exogenous particles, including fluorescent particles. Upon excitation at different wavelengths, these fluorescent species bring about background noises and interferences covering nearly the whole visible region and therefore render many intracellular targets unaddressable. The oscillating NIR fluorescence signal with an on/off ratio of up to 67 that the polymer nanoparticles display is beyond the typical background noises and interferences, thus producing superior sharpness, reliability, and signal-to-noise ratios in cellular imaging. Taking these salient features, we anticipate that these types of nanoparticles will be useful for in vivo imaging of biological tissue and other complex specimens, where two-photon activation and excitation are used in combination with NIR-fluorescence photoswitching.

Reflectance Photoplethysmography as Noninvasive Monitoring of Tissue Blood Perfusion.

PubMed

Abay, Tomas Ysehak; Kyriacou, Panayiotis A

2015-09-01

In the last decades, photoplethysmography (PPG) has been used as a noninvasive technique for monitoring arterial oxygen saturation by pulse oximetry (PO), whereas near-infrared spectroscopy (NIRS) has been employed for monitoring tissue blood perfusion. While NIRS offers more parameters to evaluate oxygen delivery and consumption in deep tissues, PO only assesses the state of oxygen delivery. For a broader assessment of blood perfusion, this paper explores the utilization of dual-wavelength PPG by using the pulsatile (ac) and continuous (dc) PPG for the estimation of arterial oxygen saturation (SpO2) by conventional PO. Additionally, the Beer-Lambert law is applied to the dc components only for the estimation of changes in deoxyhemoglobin (HHb), oxyhemoglobin (HbO2), and total hemoglobin (tHb) as in NIRS. The system was evaluated on the forearm of 21 healthy volunteers during induction of venous occlusion (VO) and total occlusion (TO). A reflectance PPG probe and NIRS sensor were applied above the brachioradialis, PO sensors were applied on the fingers, and all the signals were acquired simultaneously. While NIRS and forearm SpO2 indicated VO, SpO2 from the finger did not exhibit any significant drop from baseline. During TO, all the indexes indicated the change in blood perfusion. HHb, HbO2, and tHb changes estimated by PPG presented high correlation with the same parameters obtained by NIRS during VO (r(2) = 0.960, r(2) = 0.821, and r(2) = 0.974, respectively) and during TO (r(2) = 0.988, r(2) = 0.940, and r(2) = 0.938, respectively). The system demonstrated the ability to extract valuable information from PPG signals for a broader assessment of tissue blood perfusion.

The case for a modern multiwavelength, polarization-sensitive LIDAR in orbit around Mars

USGS Publications Warehouse

Brown, Adrian J.; Michaels, Timothy I.; Byrne, Shane; Sun, Wenbo; Titus, Timothy N.; Colaprete, Anthony; Wolff, Michael J.; Videen, Gorden; Grund, Christian J.

2014-01-01

We present the scientific case to build a multiple-wavelength, active, near-infrared (NIR) instrument to measure the reflected intensity and polarization characteristics of backscattered radiation from planetary surfaces and atmospheres. We focus on the ability of such an instrument to enhance, perhaps revolutionize, our understanding of climate, volatiles and astrobiological potential of modern-day Mars.

High Transparent Conductive Aluminum-Doped Zinc Oxide Thin Films by Reactive Co-Sputtering (Postprint)

DTIC Science & Technology

2016-03-30

wavelength where n = k) is 1605 nm from the film (f). Figure 1 XRD patterns of the AZO films on quartz substrate Figure 2 UV-Vis-NIR...71.6 1605 9.87 x10 -4 Figure 3 Refractive index n (left) and extinction coefficient k of (right) the AZO films. 4. Conclusions AZO films were

[Measurement of soil organic matter and available K based on SPA-LS-SVM].

PubMed

Zhang, Hai-Liang; Liu, Xue-Mei; He, Yong

2014-05-01

Visible and short wave infrared spectroscopy (Vis/SW-NIRS) was investigated in the present study for measurement of soil organic matter (OM) and available potassium (K). Four types of pretreatments including smoothing, SNV, MSC and SG smoothing+first derivative were adopted to eliminate the system noises and external disturbances. Then partial least squares regression (PLSR) and least squares-support vector machine (LS-SVM) models were implemented for calibration models. The LS-SVM model was built by using characteristic wavelength based on successive projections algorithm (SPA). Simultaneously, the performance of LSSVM models was compared with PLSR models. The results indicated that LS-SVM models using characteristic wavelength as inputs based on SPA outperformed PLSR models. The optimal SPA-LS-SVM models were achieved, and the correlation coefficient (r), and RMSEP were 0. 860 2 and 2. 98 for OM and 0. 730 5 and 15. 78 for K, respectively. The results indicated that visible and short wave near infrared spectroscopy (Vis/SW-NIRS) (325 approximately 1 075 nm) combined with LS-SVM based on SPA could be utilized as a precision method for the determination of soil properties.

Nonlinear optical detection of terahertz-wave radiation from resonant tunneling diodes.

PubMed

Takida, Yuma; Nawata, Kouji; Suzuki, Safumi; Asada, Masahiro; Minamide, Hiroaki

2017-03-06

The sensitive detection of terahertz (THz)-wave radiation from compact sources at room temperature is crucial for real-world THz-wave applications. Here, we demonstrate the nonlinear optical detection of THz-wave radiation from continuous-wave (CW) resonant tunneling diodes (RTDs) at 0.58, 0.78, and 1.14 THz. The up-conversion process in a MgO:LiNbO₃ crystal under the noncollinear phase-matching condition offers efficient wavelength conversion from a THz wave to a near-infrared (NIR) wave that is detected using a commercial NIR photodetector. The minimum detection limit of CW THz-wave power is as low as 5 nW at 1.14 THz, corresponding to 2-aJ energy and 2.7 × 10³ photons within the time window of a 0.31-ns pump pulse. Our results show that the input frequency and power of RTD devices can be calibrated by measuring the output wavelength and energy of up-converted waves, respectively. This optical detection technique for compact electronic THz-wave sources will open up a new opportunity for the realization of real-world THz-wave applications.

Solar rotational modulations of spectral irradiance and correlations with the variability of total solar irradiance

NASA Astrophysics Data System (ADS)

Lee, Jae N.; Cahalan, Robert F.; Wu, Dong L.

2016-09-01

Aims: We characterize the solar rotational modulations of spectral solar irradiance (SSI) and compare them with the corresponding changes of total solar irradiance (TSI). Solar rotational modulations of TSI and SSI at wavelengths between 120 and 1600 nm are identified over one hundred Carrington rotational cycles during 2003-2013. Methods: The SORCE (Solar Radiation and Climate Experiment) and TIMED (Thermosphere Ionosphere Mesosphere Energetics and Dynamics)/SEE (Solar EUV Experiment) measured and SATIRE-S modeled solar irradiances are analyzed using the EEMD (Ensemble Empirical Mode Decomposition) method to determine the phase and amplitude of 27-day solar rotational variation in TSI and SSI. Results: The mode decomposition clearly identifies 27-day solar rotational variations in SSI between 120 and 1600 nm, and there is a robust wavelength dependence in the phase of the rotational mode relative to that of TSI. The rotational modes of visible (VIS) and near infrared (NIR) are in phase with the mode of TSI, but the phase of the rotational mode of ultraviolet (UV) exhibits differences from that of TSI. While it is questionable that the VIS to NIR portion of the solar spectrum has yet been observed with sufficient accuracy and precision to determine the 11-year solar cycle variations, the temporal variations over one hundred cycles of 27-day solar rotation, independent of the two solar cycles in which they are embedded, show distinct solar rotational modulations at each wavelength.

Solar Rotational Modulations of Spectral Irradiance and Correlations with the Variability of Total Solar Irradiance

NASA Technical Reports Server (NTRS)

Lee, Jae N.; Cahalan, Robert F.; Wu, Dong L.

2016-01-01

Aims: We characterize the solar rotational modulations of spectral solar irradiance (SSI) and compare them with the corresponding changes of total solar irradiance (TSI). Solar rotational modulations of TSI and SSI at wavelengths between 120 and 1600 nm are identified over one hundred Carrington rotational cycles during 2003-2013. Methods: The SORCE (Solar Radiation and Climate Experiment) and TIMED (Thermosphere Ionosphere Mesosphere Energetics and Dynamics)/SEE (Solar EUV Experiment) measured and SATIRE-S modeled solar irradiances are analyzed using the EEMD (Ensemble Empirical Mode Decomposition) method to determine the phase and amplitude of 27-day solar rotational variation in TSI and SSI. Results: The mode decomposition clearly identifies 27-day solar rotational variations in SSI between 120 and 1600 nm, and there is a robust wavelength dependence in the phase of the rotational mode relative to that of TSI. The rotational modes of visible (VIS) and near infrared (NIR) are in phase with the mode of TSI, but the phase of the rotational mode of ultraviolet (UV) exhibits differences from that of TSI. While it is questionable that the VIS to NIR portion of the solar spectrum has yet been observed with sufficient accuracy and precision to determine the 11-year solar cycle variations, the temporal variations over one hundred cycles of 27-day solar rotation, independent of the two solar cycles in which they are embedded, show distinct solar rotational modulations at each wavelength.

Recent Advances in Inorganic Nanoparticle-Based NIR Luminescence Imaging: Semiconductor Nanoparticles and Lanthanide Nanoparticles.

PubMed

Kim, Dokyoon; Lee, Nohyun; Park, Yong Il; Hyeon, Taeghwan

2017-01-18

Several types of nanoparticle-based imaging probes have been developed to replace conventional luminescent probes. For luminescence imaging, near-infrared (NIR) probes are useful in that they allow deep tissue penetration and high spatial resolution as a result of reduced light absorption/scattering and negligible autofluorescence in biological media. They rely on either an anti-Stokes or a Stokes shift process to generate luminescence. For example, transition metal-doped semiconductor nanoparticles and lanthanide-doped inorganic nanoparticles have been demonstrated as anti-Stokes shift-based agents that absorb NIR light through two- or three-photon absorption process and upconversion process, respectively. On the other hand, quantum dots (QDs) and lanthanide-doped nanoparticles that emit in NIR-II range (∼1000 to ∼1350 nm) were suggested as promising Stokes shift-based imaging agents. In this topical review, we summarize and discuss the recent progress in the development of inorganic nanoparticle-based luminescence imaging probes working in NIR range.

Seasonal trends in separability of leaf reflectance spectra for Ailanthus altissima and four other tree species

NASA Astrophysics Data System (ADS)

Burkholder, Aaron

This project investigated the spectral separability of the invasive species Ailanthus altissima, commonly called tree of heaven, and four other native species. Leaves were collected from Ailanthus and four native tree species from May 13 through August 24, 2008, and spectral reflectance factor measurements were gathered for each tree using an ASD (Boulder, Colorado) FieldSpec Pro full-range spectroradiometer. The original data covered the range from 350-2500 nm, with one reflectance measurement collected per one nm wavelength. To reduce dimensionality, the measurements were resampled to the actual resolution of the spectrometer's sensors, and regions of atmospheric absorption were removed. Continuum removal was performed on the reflectance data, resulting in a second dataset. For both the reflectance and continuum removed datasets, least angle regression (LARS) and random forest classification were used to identify a single set of optimal wavelengths across all sampled dates, a set of optimal wavelengths for each date, and the dates for which Ailanthus is most separable from other species. It was found that classification accuracy varies both with dates and bands used. Contrary to expectations that early spring would provide the best separability, the lowest classification error was observed on July 22 for the reflectance data, and on May 13, July 11 and August 1 for the continuum removed data. This suggests that July and August are also potentially good months for species differentiation. Applying continuum removal in many cases reduced classification error, although not consistently. Band selection seems to be more important for reflectance data in that it results in greater improvement in classification accuracy, and LARS appears to be an effective band selection tool. The optimal spectral bands were selected from across the spectrum, often with bands from the blue (401-431 nm), NIR (1115 nm) and SWIR (1985-1995 nm), suggesting that hyperspectral sensors with broad wavelength sensitivity are important for mapping and identification of Ailanthus.

Laser-based optoelectronic system for therapy by medical treatment of cardiovascular diseases

NASA Astrophysics Data System (ADS)

Chtchoupak, Oleg S.; Spilevoi, Boris N.; Zapaeva, Natlia L.

1996-04-01

The method and system's design for the laser treatment of the heart ischemia is presented. Our conceptual approach to the development of the system is based on the theoretical and experimental works about positive influence of low intensity near infrared laser irradiation by treatment of cardiovascular diseases. The method and system allow it to influence the subepicardial collateral blood circulation with near infrared (NIR) laser irradiation in wavelength ranges of 0.86 - 1.06 mkm. The presented techniques make it possible to achieve a higher effectiveness of treatment. First, due to individual choice of radiation parameters on the basis of analysis of the patient's conditions before and after laser therapy. Second, due to simultaneous influence at several points of the human body. Finally, results of the clinical tests are presented, which confirm the discussed methods.

Fiber Lasers and Amplifiers for Space-based Science and Exploration

NASA Technical Reports Server (NTRS)

Yu, Anthony W.; Krainak, Michael A.; Stephen, Mark A.; Chen, Jeffrey R.; Coyle, Barry; Numata, Kenji; Camp, Jordan; Abshire, James B.; Allan, Graham R.; Li, Steven X.;

[Micro Hadamard transform near-infrared spectrometer].

PubMed

Zhang, Zhi-hai; Muo, Xiang-xia; Guo, Yuan-jun; Wang, Wei

2011-07-01

A new type micro Hadamard transform (HT) near-infrared (NIR) spectrometer is proposed in the present paper. It has a MOEMS (Micro-Opto-Electro-Mechanical Systems) blazed grating HT mask. It has merits of compactness, agility of dynamic mask generation and high scan speed. The structure and theory of this spectrometer are analyzed. The 63-order Hadamard-S matrix and mask are designed. The mask is dynamically generated by program of MOEMS blazed gratings. The spectrum is in agreement with that measured by Shimadzu spectrometer in experiments. It has a wavelength range between 900 and 1 700 nm, spectral resolution of 19 nm, single scan time of 2.4 s, SNR of 44.67:1, optical path of 70 mm x 130 mm, and weight under 1 kg. It can meet the requirement of real time detection and portable application.