A spatially offset Raman spectroscopy method for non-destructive detection of gelatin-encapsulated powders

USDA-ARS?s Scientific Manuscript database

Non-destructive subsurface detection of encapsulated, coated, or seal-packaged foods and pharmaceuticals can help prevent distribution and consumption of counterfeit or hazardous products. This study used a Spatially Offset Raman Spectroscopy (SORS) method to detect and identify urea, ibuprofen, and...

Non-destructive testing method and apparatus

DOEpatents

Akers, Douglas W [Idaho Falls, ID

2011-10-04

Non-destructive testing apparatus may comprise a photon source and a source material that emits positrons in response to bombardment of the source material with photons. The source material is positionable adjacent the photon source and a specimen so that when the source material is positioned adjacent the photon source it is exposed to photons produced thereby. When the source material is positioned adjacent the specimen, the specimen is exposed to at least some of the positrons emitted by the source material. A detector system positioned adjacent the specimen detects annihilation gamma rays emitted by the specimen. Another embodiment comprises a neutron source and a source material that emits positrons in response to neutron bombardment.

Quantitative non-destructive assay of PuBe neutron sources

NASA Astrophysics Data System (ADS)

Lakosi, László; Bagi, János; Nguyen, Cong Tam

2006-02-01

PuBe neutron sources were assayed, using a combination of high resolution γ-spectrometry (HRGS) and neutron correlation technique. In a previous publication [J. Bagi, C. Tam Nguyen, L. Lakosi, Nucl. Instr. and Meth. B 222 (2004) 242] a passive neutron well-counter was reported with 3He tubes embedded in a polyamide (TERRAMID) moderator (lined inside with Cd) surrounding the sources to be measured. Gross and coincidence neutron counting was performed, and the Pu content of the sources was found out from isotope analysis and by adopting specific (α, n) reaction yields of the Pu isotopes and 241Am in Be, based on supplier's information and literature data. The method was further developed and refined. Evaluation algorithm was more precisely worked out. The contribution of secondary (correlated) neutrons to the total neutron output was derived from the coincidence (doubles) count rate and taken into account in assessing the Pu content. A new evaluation of former results was performed. Assay was extended to other PuBe sources, and new results were added. In order to attain higher detection efficiency, a more efficient moderator was also applied, with and without Cd shielding around the assay chamber. Calibration seems possible using neutron measurements only (without γ-spectrometry), based on a correlation between the Pu amount and the coincidence-to-total ratio. It is expected that the method could be used for Pu accountancy and safeguards verification as well as identification and assay of seized, found, or not documented PuBe neutron sources.

Non-destructive imaging of spinor Bose-Einstein condensates

NASA Astrophysics Data System (ADS)

Samson, E.; Vinit, Anshuman; Raman, Chandra

2013-05-01

We present a non-destructive differential imaging technique that enables the observation of the spatial distribution of the magnetization in a spinor Bose-Einstein condensate (BEC) through a Faraday rotation protocol. In our procedure, we utilize a linearly polarized, far-detuned laser beam as our imaging probe, and upon interaction with the condensate, the beam's polarization direction undergoes Faraday rotation. A differential measurement of the orthogonal polarization components of the rotated beam provides a spatial map of the net magnetization density within the BEC. The non-destructive aspect of this method allows for continuous imaging of the condensate. This imaging technique will prove useful in experimental BEC studies, such as spatially resolved magnetometry using ultracold atoms, and non-destructive imaging of non-equilibrium behavior of antiferromagnetic spinor condensates. This work was supported by the DARPA QuASAR program through a grant from ARO.

Complementary Electromagnetic Non-Destructive Evaluation

NASA Astrophysics Data System (ADS)

Tian, Gui Yun; Wilson, John; Morozov, Maxim

2011-06-01

The use of non-destructive evaluation (NDE) for defect detection and failure prediction in structures and specimens is widespread in energy industries, aimed at ageing power plants and pipelines, material degradation, fatigue and radiation damage, etc. At present there are no suitable electromagnetic NDE methods for the measurement and characterization of material degradation, in irradiated samples in particular, which is very important and timely for the nuclear power industry in the UK. This paper reports recent developments in the field of electromagnetic (EM) NDE at Newcastle University, including pulsed eddy current (PEC), pulsed magnetic flux leakage (PMFL), magnetic Barkhausen emission (MBE) and magneto-acoustic emission (MAE). As different EM methods have different strengths, an integrative EM framework is introduced. Case studies through the second round robin tests organized by the Universal Network for Magnetic Non-Destructive Evaluation (UNMNDE), representing eighteen leading research groups worldwide in the area of electromagnetic NDE, are reported. Twelve samples with different ageing times and rolling reduction ratios were tested using different magnetic methods among the UNMNDE members. Based on the studies, the complementary characteristics of electromagnetic techniques for NDE are discussed.

Non-destructive examination system of vitreous body

NASA Astrophysics Data System (ADS)

Shibata, Takuma; Gong, Jin; Watanabe, Yosuke; Kabir, M. Hasnat; Masato, Makino; Furukawa, Hidemitsu; Nishitsuka, Koichi

2014-04-01

Eyeball plays a quite important role in acquiring the vision. Vitreous body occupies the largest part of the eyeball and consists of biological, elastic, transparent, gel materials. In the present medical examination, the non-destructive examination method of the vitreous body has not been well established. Here, we focus on an application of dynamic light scattering to this topic. We tried to apply our lab-made apparatus, scanning microscopic light scattering (SMILS), which was specially designed for observing the nanometer-scale network structure in gel materials. In order to examine the vitreous body using SMILS method, a commercial apparatus, nano Partica (Horiba Co. Ltd.) was also customized. We analyzed vitreous body using both the SMILS and the customized nano Partica. We successfully examined the vitreous bodies of healthy pigs in non-destructive way.

Improvement of non-destructive fissile mass assays in α low-level waste drums: A matrix correction method based on neutron capture gamma-rays and a neutron generator

NASA Astrophysics Data System (ADS)

Jallu, F.; Loche, F.

2008-08-01

Within the framework of radioactive waste control, non-destructive assay (NDA) methods may be employed. The active neutron interrogation (ANI) method is now well-known and effective in quantifying low α-activity fissile masses (mainly 235U, 239Pu, 241Pu) with low densities, i.e. less than about 0.4, in radioactive waste drums of volumes up to 200 l. The PROMpt Epithermal and THErmal interrogation Experiment (PROMETHEE [F. Jallu, A. Mariani, C. Passard, A.-C. Raoux, H. Toubon, Alpha low level waste control: improvement of the PROMETHEE 6 assay system performances. Nucl. Technol. 153 (January) (2006); C. Passard, A. Mariani, F. Jallu, J. Romeyer-Dherber, H. Recroix, M. Rodriguez, J. Loridon, C. Denis, PROMETHEE: an alpha low level waste assay system using passive and active neutron measurement methods. Nucl. Technol. 140 (December) (2002) 303-314]) based on ANI has been under development since 1996 to reach the incinerating α low level waste (LLW) criterion of about 50 Bq[α] per gram of crude waste (≈50 μg Pu) in 118 l drums on the date the drums are conditioned. Difficulties arise when dealing with matrices containing neutron energy moderators such as H and neutron absorbents such as Cl. These components may have a great influence on the fissile mass deduced from the neutron signal measured by ANI. For example, the calibration coefficient measured in a 118 l drum containing a cellulose matrix (density d = 0.144 g cm -3) may be 50 times higher than that obtained in a poly-vinyl-chloride matrix ( d = 0.253 g cm -3). Without any information on the matrix, the fissile mass is often overestimated due to safety procedures and by considering the most disadvantageous calibration coefficient corresponding to the most absorbing and moderating calibration matrix. The work discussed in this paper was performed at the CEA Nuclear Measurement Laboratory in France. It concerns the development of a matrix effect correction method, which consists in identifying and quantifying

Application of Non-destructive Methods of Stress-strain State at Hazardous Production Facilities

NASA Astrophysics Data System (ADS)

Shram, V.; Kravtsova, Ye; Selsky, A.; Bezborodov, Yu; Lysyannikova, N.; Lysyannikov, A.

2016-06-01

The paper deals with the sources of accidents in distillation columns, on the basis of which the most dangerous defects are detected. The analysis of the currently existing methods of non-destructive testing of the stress-strain state is performed. It is proposed to apply strain and acoustic emission techniques to continuously monitor dangerous objects, which helps prevent the possibility of accidents, as well as reduce the work.

A new facility for non-destructive assay using a 252Cf source.

PubMed

Stevanato, L; Caldogno, M; Dima, R; Fabris, D; Hao, Xin; Lunardon, M; Moretto, S; Nebbia, G; Pesente, S; Pino, F; Sajo-Bohus, L; Viesti, G

2013-03-01

A new laboratory facility for non-destructive analysis (NDA) using a time-tagged (252)Cf source is presented. The system is designed to analyze samples having maximum size of about 20 × 25 cm(2), the material recognition being obtained by measuring simultaneously total and energy dependent transmission of neutrons and gamma rays. The equipment technical characteristics and performances of the NDA system are presented, exploring also limits due to the sample thickness. Some recent applications in the field of cultural heritage are presented. Copyright © 2012 Elsevier Ltd. All rights reserved.

Non-destructive testing method for determining the solvent diffusion coefficient in the porous materials products

NASA Astrophysics Data System (ADS)

Belyaev, V. P.; Mishchenko, S. V.; Belyaev, P. S.

2018-01-01

Ensuring non-destructive testing of products in industry is an urgent task. Most of the modern methods for determining the diffusion coefficient in porous materials have been developed for bodies of a given configuration and size. This leads to the need for finished products destruction to make experimental samples from them. The purpose of this study is the development of a dynamic method that allows operatively determine the diffusion coefficient in finished products from porous materials without destroying them. The method is designed to investigate the solvents diffusion coefficient in building constructions from materials having a porous structure: brick, concrete and aerated concrete, gypsum, cement, gypsum or silicate solutions, gas silicate blocks, heat insulators, etc. A mathematical model of the method is constructed. The influence of the design and measuring device operating parameters on the method accuracy is studied. The application results of the developed method for structural porous products are presented.

Measurement of the 235U Induced Fission Gamma-ray Spectrum as an Active Non-destructive Assay of Fresh Nucleear Fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sarnoski, Sarah E.; Fast, James E.; Fulsom, Bryan G.

2017-07-17

Non-destructive assay is a powerful tool the International Atomic Energy Agency (IAEA) employs to verify adherence to safeguards agreements. Current IAEA veri- cation techniques for fresh nuclear fuel include passive gamma-ray spectroscopy to determine fuel enrichment. This technique suers from self-shielding and lakes the percision to detect diversion of central fuel rods. The aim of this research is to develop a new, more capable non-destructive analysis technique using active neutron interroga- tion of fuel assemblies and determining the yields of short-lived ssion products from high-resolution gamma-ray spectroscopy using high-purity germanium (HPGe). This paper reports results from irradiation of a onemore » meter tall mock fresh fuel assembly with low enriched uranium (LEU) or depleted uranium (DU) rods using a down-scattered deuterium-tritium (D-T) neutron source. Both prompt and delayed gamma-ray spec- tra were collected as time-stamped list-mode data in a coax detector and without list mode data in a planar strip detector. No dierentiating signatures were observed in the prompt spectra in either detector; however, both detectors observed several short-lived ssion product signatures in LEU and not DU fuel, indicating that this technique has potential for determination of enrichment of fresh fuel assemblies. There were eight unique ssion products observed in the LEU spectra with the coax detector spectra, and three ssion products were observed in the LEU spectra with the strip detector.« less

Non-destructive testing principles and accurate evaluation of the hydraulic measure impact range using the DC method

NASA Astrophysics Data System (ADS)

Qiu, Liming; Shen, Rongxi; Song, Dazhao; Wang, Enyuan; Liu, Zhentang; Niu, Yue; Jia, Haishan; Xia, Shankui; Zheng, Xiangxin

2017-12-01

An accurate and non-destructive evaluation method for the hydraulic measure impact range in coal seams is urgently needed. Aiming at the application demands, a theoretical study and field test are presented using the direct current (DC) method to evaluate the impact range of coal seam hydraulic measures. We firstly analyzed the law of the apparent resistivity response of an abnormal conductive zone in a coal seam, and then investigated the principle of non-destructive testing of the coal seam hydraulic measure impact range using the DC method, and used an accurate evaluation method based on the apparent resistivity cloud chart. Finally, taking hydraulic fracturing and hydraulic flushing as examples, field experiments were carried out in coal mines to evaluate the impact ranges. The results showed that: (1) in the process of hydraulic fracturing, coal conductivity was enhanced by high-pressure water in the coal seam, and after hydraulic fracturing, the boundary of the apparent resistivity decrease area was the boundary impact range. (2) In the process of hydraulic flushing, coal conductivity was reduced by holes and cracks in the coal seam, and after hydraulic flushing, the boundary of the apparent resistivity increase area was the boundary impact range. (3) After the implementation of the hydraulic measures, there may be some blind zones in the coal seam; in hydraulic fracturing blind zones, the apparent resistivity increased or stayed constant, while in hydraulic flushing blind zones, the apparent resistivity decreased or stayed constant. The DC method realized a comprehensive and non-destructive evaluation of the impact range of the hydraulic measures, and greatly reduced the time and cost of evaluation.

Development of vibrational spectroscopic methods to rapidly and non-destructively assess quality of chicken breast meat

USDA-ARS?s Scientific Manuscript database

Development of Vibrational Spectroscopic Methods to Rapidly and Non-Destructively Assess Quality of Chicken Breast Meat H. Zhuang1, M. Sohn2, S. Trabelsi1 and K. Lawrence1 1Quality and Safety Assessment Research Unit, ARS-USDA, 950 College Station Road, Athens, GA 30605 2University of Georgia, De...

Laser active thermography for non-destructive testing

NASA Astrophysics Data System (ADS)

Semerok, A.; Grisolia, C.; Fomichev, S. V.; Thro, P.-Y.

2013-11-01

Thermography methods have found their applications in different fields of human activity. The non-destructive feature of these methods along with the additional advantage by automated remote control and tests of nuclear installations without personnel attendance in the contaminated zone are of particular interest. Laser active pyrometry and laser lock-in thermography for in situ non-destructive characterization of micrometric layers on graphite substrates from European tokamaks were under extensive experimental and theoretical studies in CEA (France). The studies were aimed to obtain layer characterization with cross-checking the layer thermal contact coefficients determined by active laser pyrometry and lock-in thermography. The experimental installation comprised a Nd-YAG pulsed repetition rate laser (1 Hz - 10 kHz repetition rate frequency, homogeneous spot) and a home-made pyrometer system based on two pyrometers for the temperature measurements in 500 - 2600 K range. For both methods, the layer characterization was provided by the best fit of the experimental results and simulations. The layer thermal contact coefficients determined by both methods were quite comparable. Though there was no gain in the measurements accuracy, lock-in measurements have proved their advantage as being much more rapid. The obtained experimental and theoretical results are presented. Some practical applications and possible improvements of the methods are discussed.

Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines.

PubMed

Busschots, Steven; O'Toole, Sharon; O'Leary, John J; Stordal, Britta

2015-01-01

Many protocols used for measuring the growth of adherent monolayer cells in vitro are invasive, destructive and do not allow for the continued, undisturbed growth of cells within flasks. Protocols often use indirect methods for measuring proliferation. Microscopy techniques can analyse cell proliferation in a non-invasive or non-destructive manner but often use expensive equipment and software algorithms. In this method images of cells within flasks are captured by photographing under a standard inverted phase contract light microscope using a digital camera with a camera lens adaptor. Images are analysed for confluence using ImageJ freeware resulting in a measure of confluence known as an Area Fraction (AF) output. An example of the AF method in use on OVCAR8 and UPN251 cell lines is included. •Measurements of confluence from growing adherent cell lines in cell culture flasks is obtained in a non-invasive, non-destructive, label-free manner.•The technique is quick, affordable and eliminates sample manipulation.•The technique provides an objective, consistent measure of when cells reach confluence and is highly correlated to manual counting with a haemocytometer. The average correlation co-efficient from a Spearman correlation (n = 3) was 0.99 ± 0.008 for OVCAR8 (p = 0.01) and 0.99 ± 0.01 for UPN251 (p = 0.01) cell lines.

A Spatially Offset Raman Spectroscopy Method for Non-Destructive Detection of Gelatin-Encapsulated Powders

PubMed Central

Chao, Kuanglin; Dhakal, Sagar; Qin, Jianwei; Peng, Yankun; Schmidt, Walter F.; Kim, Moon S.; Chan, Diane E.

2017-01-01

Non-destructive subsurface detection of encapsulated, coated, or seal-packaged foods and pharmaceuticals can help prevent distribution and consumption of counterfeit or hazardous products. This study used a Spatially Offset Raman Spectroscopy (SORS) method to detect and identify urea, ibuprofen, and acetaminophen powders contained within one or more (up to eight) layers of gelatin capsules to demonstrate subsurface chemical detection and identification. A 785-nm point-scan Raman spectroscopy system was used to acquire spatially offset Raman spectra for an offset range of 0 to 10 mm from the surfaces of 24 encapsulated samples, using a step size of 0.1 mm to obtain 101 spectral measurements per sample. As the offset distance was increased, the spectral contribution from the subsurface powder gradually outweighed that of the surface capsule layers, allowing for detection of the encapsulated powders. Containing mixed contributions from the powder and capsule, the SORS spectra for each sample were resolved into pure component spectra using self-modeling mixture analysis (SMA) and the corresponding components were identified using spectral information divergence values. As demonstrated here for detecting chemicals contained inside thick capsule layers, this SORS measurement technique coupled with SMA has the potential to be a reliable non-destructive method for subsurface inspection and authentication of foods, health supplements, and pharmaceutical products that are prepared or packaged with semi-transparent materials. PMID:28335453

Evaluation of non-destructive methods for estimating biomass in marshes of the upper Texas, USA coast

USGS Publications Warehouse

Whitbeck, M.; Grace, J.B.

2006-01-01

The estimation of aboveground biomass is important in the management of natural resources. Direct measurements by clipping, drying, and weighing of herbaceous vegetation are time-consuming and costly. Therefore, non-destructive methods for efficiently and accurately estimating biomass are of interest. We compared two non-destructive methods, visual obstruction and light penetration, for estimating aboveground biomass in marshes of the upper Texas, USA coast. Visual obstruction was estimated using the Robel pole method, which primarily measures the density and height of the canopy. Light penetration through the canopy was measured using a Decagon light wand, with readings taken above the vegetation and at the ground surface. Clip plots were also taken to provide direct estimates of total aboveground biomass. Regression relationships between estimated and clipped biomass were significant using both methods. However, the light penetration method was much more strongly correlated with clipped biomass under these conditions (R2 value 0.65 compared to 0.35 for the visual obstruction approach). The primary difference between the two methods in this situation was the ability of the light-penetration method to account for variations in plant litter. These results indicate that light-penetration measurements may be better for estimating biomass in marshes when plant litter is an important component. We advise that, in all cases, investigators should calibrate their methods against clip plots to evaluate applicability to their situation. ?? 2006, The Society of Wetland Scientists.

Edward's sword? - A non-destructive study of a medieval king's sword

NASA Astrophysics Data System (ADS)

Segebade, Chr.

2013-04-01

Non-destructive and instrumental methods including photon activation analysis were applied in an examination of an ancient sword. It was tried to find indication of forgery or, if authentic, any later processing and alteration. Metal components of the hilt and the blade were analysed by instrumental photon activation. Non-destructive metallurgical studies (hardness measurements, microscopic microstructure analysis) are briefly described, too. The results of these investigations did not yield indication of non-authenticity. This stood in agreement with the results of stylistic and scientific studies by weapon experts.

Edward's sword? - A non-destructive study of a medieval king's sword

DOE Office of Scientific and Technical Information (OSTI.GOV)

Segebade, Chr.

2013-04-19

Non-destructive and instrumental methods including photon activation analysis were applied in an examination of an ancient sword. It was tried to find indication of forgery or, if authentic, any later processing and alteration. Metal components of the hilt and the blade were analysed by instrumental photon activation. Non-destructive metallurgical studies (hardness measurements, microscopic microstructure analysis) are briefly described, too. The results of these investigations did not yield indication of non-authenticity. This stood in agreement with the results of stylistic and scientific studies by weapon experts.

Non-destructive method for determining neutron exposure and constituent concentrations of a body

DOEpatents

Gold, Raymond; McElroy, William N.

1986-01-01

A non-destructive method for determination of neutron exposure and constituent concentrations in an object, such as reactor pressure vessel, is based on the observation of characteristic gamma-rays emitted by activation products in the object by using a unique continuous gamma-ray spectrometer. The spectrometer views the object through appropriate collimators to determine the absolute emission rate of these characteristic gamma-rays, thereby ascertaining the absolute activity of given activation products in the object. These data can then be used to deduce the spatial and angular dependence of neutron exposure or the spatial constituent concentration at regions of interest within the object.

Development of a neutron measurement system in unified non-destructive assay for the PRIDE facility

NASA Astrophysics Data System (ADS)

Seo, Hee; Park, Se-Hwan; Won, Byung-Hee; Ahn, Seong-Kyu; Shin, Hee-Sung; Na, Sang-Ho; Song, Dae-Yong; Kim, Ho-Dong; Lee, Seung Kyu

2013-12-01

The Korea Atomic Energy Research Institute (KAERI) has made an effort to develop pyroprocessing technology to resolve an on-going problem in Korea, i.e., the management of spent nuclear fuels. To this end, a test-bed facility for pyroprocessing, called PRIDE (PyRoprocessing Integrated inactive DEmonstration facility), is being constructed at KAERI. The main objective of PRIDE is to evaluate the performance of the unit processes, remote operation, maintenance, and proliferation resistance. In addition, integrating all unit processes into a one-step process is also one of the main goals. PRIDE can also provide a good opportunity to test safeguards instrumentations for a pyroprocessing facility such as nuclear material accounting devices, surveillance systems, radiation monitoring systems, and process monitoring systems. In the present study, a non-destructive assay (NDA) system for the testing of nuclear material accountancy of PRIDE was designed by integrating three different NDA techniques, i.e., neutron, gamma-ray, and mass measurements. The developed neutron detection module consists of 56 3He tubes and 16 AMPTEK A111 signal processing circuits. The amplifiers were matched in terms of the gain and showed good uniformity after a gain-matching procedure (%RSD=0.37%). The axial and the radial efficiency distributions within the cavity were then measured using a 252Cf neutron source and were compared with the MCNPX calculation results. The measured efficiency distributions showed excellent agreement with the calculations, which confirmed the accuracy of the MCNPX model of the system.

Atlantis Non-destructive Testing

NASA Image and Video Library

2003-10-29

In the Orbiter Processing Facility, the nose cap (foreground) removed from Atlantis (behind) waits to be shipped to the original manufacturing company, Vought in Ft. Worth, Texas, a subsidiary of Lockheed Martin, to undergo non-destructive testing such as CAT scan and thermography.

Non-destructive lichen biomass estimation in northwestern Alaska: a comparison of methods.

PubMed

Rosso, Abbey; Neitlich, Peter; Smith, Robert J

2014-01-01

Terrestrial lichen biomass is an important indicator of forage availability for caribou in northern regions, and can indicate vegetation shifts due to climate change, air pollution or changes in vascular plant community structure. Techniques for estimating lichen biomass have traditionally required destructive harvesting that is painstaking and impractical, so we developed models to estimate biomass from relatively simple cover and height measurements. We measured cover and height of forage lichens (including single-taxon and multi-taxa "community" samples, n = 144) at 73 sites on the Seward Peninsula of northwestern Alaska, and harvested lichen biomass from the same plots. We assessed biomass-to-volume relationships using zero-intercept regressions, and compared differences among two non-destructive cover estimation methods (ocular vs. point count), among four landcover types in two ecoregions, and among single-taxon vs. multi-taxa samples. Additionally, we explored the feasibility of using lichen height (instead of volume) as a predictor of stand-level biomass. Although lichen taxa exhibited unique biomass and bulk density responses that varied significantly by growth form, we found that single-taxon sampling consistently under-estimated true biomass and was constrained by the need for taxonomic experts. We also found that the point count method provided little to no improvement over ocular methods, despite increased effort. Estimated biomass of lichen-dominated communities (mean lichen cover: 84.9±1.4%) using multi-taxa, ocular methods differed only nominally among landcover types within ecoregions (range: 822 to 1418 g m-2). Height alone was a poor predictor of lichen biomass and should always be weighted by cover abundance. We conclude that the multi-taxa (whole-community) approach, when paired with ocular estimates, is the most reasonable and practical method for estimating lichen biomass at landscape scales in northwest Alaska.

Non-Destructive Lichen Biomass Estimation in Northwestern Alaska: A Comparison of Methods

PubMed Central

Rosso, Abbey; Neitlich, Peter; Smith, Robert J.

2014-01-01

Terrestrial lichen biomass is an important indicator of forage availability for caribou in northern regions, and can indicate vegetation shifts due to climate change, air pollution or changes in vascular plant community structure. Techniques for estimating lichen biomass have traditionally required destructive harvesting that is painstaking and impractical, so we developed models to estimate biomass from relatively simple cover and height measurements. We measured cover and height of forage lichens (including single-taxon and multi-taxa “community” samples, n = 144) at 73 sites on the Seward Peninsula of northwestern Alaska, and harvested lichen biomass from the same plots. We assessed biomass-to-volume relationships using zero-intercept regressions, and compared differences among two non-destructive cover estimation methods (ocular vs. point count), among four landcover types in two ecoregions, and among single-taxon vs. multi-taxa samples. Additionally, we explored the feasibility of using lichen height (instead of volume) as a predictor of stand-level biomass. Although lichen taxa exhibited unique biomass and bulk density responses that varied significantly by growth form, we found that single-taxon sampling consistently under-estimated true biomass and was constrained by the need for taxonomic experts. We also found that the point count method provided little to no improvement over ocular methods, despite increased effort. Estimated biomass of lichen-dominated communities (mean lichen cover: 84.9±1.4%) using multi-taxa, ocular methods differed only nominally among landcover types within ecoregions (range: 822 to 1418 g m−2). Height alone was a poor predictor of lichen biomass and should always be weighted by cover abundance. We conclude that the multi-taxa (whole-community) approach, when paired with ocular estimates, is the most reasonable and practical method for estimating lichen biomass at landscape scales in northwest Alaska. PMID:25079228

Non destructive examination of interface of molecular assembly

NASA Astrophysics Data System (ADS)

Perez, Guy; Richard, Isaline; Lecomte, Jean-Claude

2017-11-01

Molecular assembly interfaces can be characterised by mechanical testing and/or the interaction between waves and the interface. The disadvantage of the mechanical approach is that new defects may be produced at the interface, or existing defects may be destroyed. Using the interaction between waves and the interface is a non-destructive approach. But what kind of waves should be used? Electromagnetic waves in the visible range depend on wave attenuation in the material, infrared waves also depend on the thickness and X-ray waves have a too short a wave length to detect interface defects. In this article, the use of acoustic waves is proposed for non-destructive examination of molecular assembly interfaces. Acoustic wave propagation is very sensitive to variations in interface characteristics depending on whether the waves are reflected or transmitted. To improve the sensitivity and resolution of this technique, small wave lengths have been used with a scanning acoustic microscope (S.A.M.) with a band width from 1MHz to 400 MHz. After a short description of the principle of the method, results are given for different types of components. Different applications of acoustic microscopy are proposed for non-destructive examination of interfaces and defect detection in materials.

Non-Destructive Sampling of Ancient Insect DNA

PubMed Central

Thomsen, Philip Francis; Elias, Scott; Gilbert, M. Thomas P.; Haile, James; Munch, Kasper; Kuzmina, Svetlana; Froese, Duane G.; Holdaway, Richard N.; Willerslev, Eske

2009-01-01

Background A major challenge for ancient DNA (aDNA) studies on insect remains is that sampling procedures involve at least partial destruction of the specimens. A recent extraction protocol reveals the possibility of obtaining DNA from past insect remains without causing visual morphological damage. We test the applicability of this protocol on historic museum beetle specimens dating back to AD 1820 and on ancient beetle chitin remains from permafrost (permanently frozen soil) dating back more than 47,000 years. Finally, we test the possibility of obtaining ancient insect DNA directly from non-frozen sediments deposited 3280-1800 years ago - an alternative approach that also does not involve destruction of valuable material. Methodology/Principal Findings The success of the methodological approaches are tested by PCR and sequencing of COI and 16S mitochondrial DNA (mtDNA) fragments of 77–204 base pairs (-bp) in size using species-specific and general insect primers. Conclusion/Significance The applied non-destructive DNA extraction method shows promising potential on insect museum specimens of historical age as far back as AD 1820, but less so on the ancient permafrost-preserved insect fossil remains tested, where DNA was obtained from samples up to ca. 26,000 years old. The non-frozen sediment DNA approach appears to have great potential for recording the former presence of insect taxa not normally preserved as macrofossils and opens new frontiers in research on ancient biodiversity. PMID:19337382

A Non-destructive Terahertz Spectroscopy-Based Method for Transgenic Rice Seed Discrimination via Sparse Representation

NASA Astrophysics Data System (ADS)

Hu, Xiaohua; Lang, Wenhui; Liu, Wei; Xu, Xue; Yang, Jianbo; Zheng, Lei

2017-08-01

Terahertz (THz) spectroscopy technique has been researched and developed for rapid and non-destructive detection of food safety and quality due to its low-energy and non-ionizing characteristics. The objective of this study was to develop a flexible identification model to discriminate transgenic and non-transgenic rice seeds based on terahertz (THz) spectroscopy. To extract THz spectral features and reduce the feature dimension, sparse representation (SR) is employed in this work. A sufficient sparsity level is selected to train the sparse coding of the THz data, and the random forest (RF) method is then applied to obtain a discrimination model. The results show that there exist differences between transgenic and non-transgenic rice seeds in THz spectral band and, comparing with Least squares support vector machines (LS-SVM) method, SR-RF is a better model for discrimination (accuracy is 95% in prediction set, 100% in calibration set, respectively). The conclusion is that SR may be more useful in the application of THz spectroscopy to reduce dimension and the SR-RF provides a new, effective, and flexible method for detection and identification of transgenic and non-transgenic rice seeds with THz spectral system.

A New Method for Non-destructive Measurement of Biomass, Growth Rates, Vertical Biomass Distribution and Dry Matter Content Based on Digital Image Analysis

PubMed Central

Tackenberg, Oliver

2007-01-01

Background and Aims Biomass is an important trait in functional ecology and growth analysis. The typical methods for measuring biomass are destructive. Thus, they do not allow the development of individual plants to be followed and they require many individuals to be cultivated for repeated measurements. Non-destructive methods do not have these limitations. Here, a non-destructive method based on digital image analysis is presented, addressing not only above-ground fresh biomass (FBM) and oven-dried biomass (DBM), but also vertical biomass distribution as well as dry matter content (DMC) and growth rates. Methods Scaled digital images of the plants silhouettes were taken for 582 individuals of 27 grass species (Poaceae). Above-ground biomass and DMC were measured using destructive methods. With image analysis software Zeiss KS 300, the projected area and the proportion of greenish pixels were calculated, and generalized linear models (GLMs) were developed with destructively measured parameters as dependent variables and parameters derived from image analysis as independent variables. A bootstrap analysis was performed to assess the number of individuals required for re-calibration of the models. Key Results The results of the developed models showed no systematic errors compared with traditionally measured values and explained most of their variance (R2 ≥ 0·85 for all models). The presented models can be directly applied to herbaceous grasses without further calibration. Applying the models to other growth forms might require a re-calibration which can be based on only 10–20 individuals for FBM or DMC and on 40–50 individuals for DBM. Conclusions The methods presented are time and cost effective compared with traditional methods, especially if development or growth rates are to be measured repeatedly. Hence, they offer an alternative way of determining biomass, especially as they are non-destructive and address not only FBM and DBM, but also vertical

Non-destructive method for determining neutron exposure and constituent concentrations of a body

DOEpatents

Gold, R.; McElroy, W.N.

1984-02-22

A non-destructive method for determination of neutron exposure and constituent concentrations in an object, such as a reactor pressure vessel, is based on the observation of characteristic gamma-rays emitted by activation products in the object by using a unique continuous gamma-ray spectrometer. The spectrometer views the object through appropriate collimators to determine the absolute emission rate of these characteristic gamma-rays, thereby ascertaining the absolute activity of given activation products in the object. These data can then be used to deduce the spatial and angular dependence of neutron exposure or the spatial constituent concentrations at regions of interest within the object.

Non-Destructive Characterization of UO2+x Nuclear Fuels

DOE PAGES

Pokharel, Reeju; Brown, Donald W.; Clausen, Bjørn; ...

2017-10-27

This article describes the effect of fabrication conditions on as-sintered microstructures of various stoichiometric ratios of uranium dioxide, UO 2+x, with the aim of enhancing the understanding of fabrication process and developing and validating a predictive microstructurebased model for fuel performance. We demonstrate the ability of novel, non-destructive methods such as near-field high-energy X-ray diffraction microscopy (nf-HEDM) and micro-computed tomography (μ-CT) to probe bulk samples of high-Z materials by non-destructively characterizing three samples: UO 2.00, UO 2.11, and UO 2.16, which were sintered at 1450°C for 4 hours. The measured 3D microstructures revealed that grain size and porosity were influencedmore » by deviation from stoichiometry.« less

A new non-destructive readout by using photo-recovered surface potential contrast

NASA Astrophysics Data System (ADS)

Wang, Le; Jin, Kui-Juan; Gu, Jun-Xing; Ma, Chao; He, Xu; Zhang, Jiandi; Wang, Can; Feng, Yu; Wan, Qian; Shi, Jin-An; Gu, Lin; He, Meng; Lu, Hui-Bin; Yang, Guo-Zhen

2014-11-01

Ferroelectric random access memory is still challenging in the feature of combination of room temperature stability, non-destructive readout and high intensity storage. As a non-contact and non-destructive information readout method, surface potential has never been paid enough attention because of the unavoidable decay of the surface potential contrast between oppositely polarized domains. That is mainly due to the recombination of the surface movable charges around the domain walls. Here, by introducing a laser beam into the combination of piezoresponse force microscopy and Kelvin probe force microscopy, we demonstrate that the surface potential contrast of BiFeO3 films can be recovered under light illumination. The recovering mechanism is understood based on the redistribution of the photo-induced charges driven by the internal electric field. Furthermore, we have created a 12-cell memory pattern based on BiFeO3 films to show the feasibility of such photo-assisted non-volatile and non-destructive readout of the ferroelectric memory.

Recent advances in the use of non-destructive near infrared spectroscopy on intact olive fruits

USDA-ARS?s Scientific Manuscript database

The objective of this review is to illustrate the state of the art in the use of non-destructive near infrared (NIR) spectroscopy for quality evaluation of intact fruit in the olive industry. First, the most recent studies regarding the application of non-destructive NIR spectroscopy methods to asse...

Quantification of nanoparticle concentration in colloidal suspensions by a non-destructive optical method

NASA Astrophysics Data System (ADS)

Clement, Sandhya; Gardner, Brint; Razali, Wan Aizuddin W.; Coleman, Victoria A.; Jämting, Åsa K.; Catchpoole, Heather J.; Goldys, Ewa M.; Herrmann, Jan; Zvyagin, Andrei

2017-11-01

The estimation of nanoparticle number concentration in colloidal suspensions is a prerequisite in many procedures, and in particular in multi-stage, low-yield reactions. Here, we describe a rapid, non-destructive method based on optical extinction and dynamic light scattering (DLS), which combines measurements using common bench-top instrumentation with a numerical algorithm to calculate the particle size distribution (PSD) and concentration. These quantities were derived from Mie theory applied to measurements of the optical extinction spectrum of homogeneous, non-absorbing nanoparticles, and the relative PSD of a colloidal suspension. The work presents an approach to account for PSDs achieved by DLS which, due to the underlying model, may not be representative of the true sample PSD. The presented approach estimates the absolute particle number concentration of samples with mono-, bi-modal and broad size distributions with <50% precision. This provides a convenient and practical solution for number concentration estimation required during many applications of colloidal nanomaterials.

Gamma neutron assay method and apparatus

DOEpatents

Cole, J.D.; Aryaeinejad, R.; Greenwood, R.C.

1995-01-03

The gamma neutron assay technique is an alternative method to standard safeguards techniques for the identification and assaying of special nuclear materials in a field or laboratory environment, as a tool for dismantlement and destruction of nuclear weapons, and to determine the isotopic ratios for a blend-down program on uranium. It is capable of determining the isotopic ratios of fissionable material from the spontaneous or induced fission of a sample to within approximately 0.5%. This is based upon the prompt coincidence relationships that occur in the fission process and the proton conservation and quasi-conservation of nuclear mass (A) that exists between the two fission fragments. The system is used in both passive (without an external neutron source) and active (with an external neutron source) mode. The apparatus consists of an array of neutron and gamma-ray detectors electronically connected to determine coincident events. The method can also be used to assay radioactive waste which contains fissile material, even in the presence of a high background radiation field. 7 figures.

Gamma neutron assay method and apparatus

DOEpatents

Cole, Jerald D.; Aryaeinejad, Rahmat; Greenwood, Reginald C.

1995-01-01

The gamma neutron assay technique is an alternative method to standard safeguards techniques for the identification and assaying of special nuclear materials in a field or laboratory environment, as a tool for dismantlement and destruction of nuclear weapons, and to determine the isotopic ratios for a blend-down program on uranium. It is capable of determining the isotopic ratios of fissionable material from the spontaneous or induced fission of a sample to within approximately 0.5%. This is based upon the prompt coincidence relationships that occur in the fission process and the proton conservation and quasi-conservation of nuclear mass (A) that exists between the two fission fragments. The system is used in both passive (without an external neutron source and active (with an external neutron source) mode. The apparatus consists of an array of neutron and gamma-ray detectors electronically connected to determine coincident events. The method can also be used to assay radioactive waste which contains fissile material, even in the presence of a high background radiation field.

Low Frequency Electrical and Magnetic Methods for Non-Destructive Analysis of Fiber Dispersion in Fiber Reinforced Cementitious Composites: An Overview

PubMed Central

Faifer, Marco; Ferrara, Liberato; Ottoboni, Roberto; Toscani, Sergio

2013-01-01

Non-destructive analysis of fiber dispersion in structural elements made of Fiber Reinforced Concrete (FRC) and Fiber Reinforced Cementitious Composites (FRCCs) plays a significant role in the framework of quality control and performance prediction. In this paper, the research activity of the authors in the aforementioned field all over the last lustrum will be reviewed. A method based on the measurement of the inductance of a probe to be placed on the specimen will be presented and its progressive development will be described. Obtained correlation with actual fiber dispersion, as checked by means of destructive methods, as well as with the mechanical performance of the composite will also be presented, in an attempt to address the significance of the method from an engineering application perspective. PMID:23337334

Low frequency electrical and magnetic methods for non-destructive analysis of fiber dispersion in fiber reinforced cementitious composites: an overview.

PubMed

Faifer, Marco; Ferrara, Liberato; Ottoboni, Roberto; Toscani, Sergio

2013-01-21

Non-destructive analysis of fiber dispersion in structural elements made of Fiber Reinforced Concrete (FRC) and Fiber Reinforced Cementitious Composites (FRCCs) plays a significant role in the framework of quality control and performance prediction. In this paper, the research activity of the authors in the aforementioned field all over the last lustrum will be reviewed. A method based on the measurement of the inductance of a probe to be placed on the specimen will be presented and its progressive development will be described. Obtained correlation with actual fiber dispersion, as checked by means of destructive methods, as well as with the mechanical performance of the composite will also be presented, in an attempt to address the significance of the method from an engineering application perspective.

Quantitative impact characterization of aeronautical CFRP materials with non-destructive testing methods

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kiefel, Denis, E-mail: Denis.Kiefel@airbus.com, E-mail: Rainer.Stoessel@airbus.com; Stoessel, Rainer, E-mail: Denis.Kiefel@airbus.com, E-mail: Rainer.Stoessel@airbus.com; Grosse, Christian, E-mail: Grosse@tum.de

2015-03-31

In recent years, an increasing number of safety-relevant structures are designed and manufactured from carbon fiber reinforced polymers (CFRP) in order to reduce weight of airplanes by taking the advantage of their specific strength into account. Non-destructive testing (NDT) methods for quantitative defect analysis of damages are liquid- or air-coupled ultrasonic testing (UT), phased array ultrasonic techniques, and active thermography (IR). The advantage of these testing methods is the applicability on large areas. However, their quantitative information is often limited on impact localization and size. In addition to these techniques, Airbus Group Innovations operates a micro x-ray computed tomography (μ-XCT)more » system, which was developed for CFRP characterization. It is an open system which allows different kinds of acquisition, reconstruction, and data evaluation. One main advantage of this μ-XCT system is its high resolution with 3-dimensional analysis and visualization opportunities, which enables to gain important quantitative information for composite part design and stress analysis. Within this study, different NDT methods will be compared at CFRP samples with specified artificial impact damages. The results can be used to select the most suitable NDT-method for specific application cases. Furthermore, novel evaluation and visualization methods for impact analyzes are developed and will be presented.« less

Performance and non-destructive evaluation methods of airborne radome and stealth structures

NASA Astrophysics Data System (ADS)

Panwar, Ravi; Ryul Lee, Jung

2018-06-01

In the past few years, great effort has been devoted to the fabrication of highly efficient, broadband radome and stealth (R&S) structures for distinct control, guidance, surveillance and communication applications for airborne platforms. The evaluation of non-planar aircraft R&S structures in terms of their electromagnetic performance and structural damage is still a very challenging task. In this article, distinct measurement techniques are discussed for the electromagnetic performance and non-destructive evaluation (NDE) of R&S structures. This paper deals with an overview of the transmission line method and free space measurement based microwave measurement techniques for the electromagnetic performance evaluation of R&S structures. In addition, various conventional as well as advanced methods, such as millimetre and terahertz wave based imaging techniques with great potential for NDE of load bearing R&S structures, are also discussed in detail. A glimpse of in situ NDE techniques with corresponding experimental setup for R&S structures is also presented. The basic concepts, measurement ranges and their instrumentation, measurement method of different R&S structures and some miscellaneous topics are discussed in detail. Some of the challenges and issues pertaining to the measurement of curved R&S structures are also presented. This study also lists various mathematical models and analytical techniques for the electromagnetic performance evaluation and NDE of R&S structures. The research directions described in this study may be of interest to the scientific community in the aerospace sectors.

Non-destructive detection and quantification of blueberry bruising using near-infrared (NIR) hyperspectral reflectance imaging

USDA-ARS?s Scientific Manuscript database

Currently, blueberry bruising is evaluated by either human visual/tactile inspection or firmness measurement instruments. These methods are destructive and time-consuming. The goal of this paper was to develop a non-destructive approach for blueberry bruising detection and quantification. The spe...

System and method for non-destructive evaluation of surface characteristics of a magnetic material

DOEpatents

Jiles, David C.; Sipahi, Levent B.

1994-05-17

A system and a related method for non-destructive evaluation of the surface characteristics of a magnetic material. The sample is excited by an alternating magnetic field. The field frequency, amplitude and offset are controlled according to a predetermined protocol. The Barkhausen response of the sample is detected for the various fields and offsets and is analyzed. The system produces information relating to the frequency content, the amplitude content, the average or RMS energy content, as well as count rate information, for each of the Barkhausen responses at each of the excitation levels applied during the protocol. That information provides a contiguous body of data, heretofore unavailable, which can be analyzed to deduce information about the surface characteristics of the material at various depths below the surface.

Method of non-destructively inspecting a curved wall portion

DOEpatents

Fong, James T.

1996-01-01

A method of non-destructively inspecting a curved wall portion of a large and thick walled vessel for a defect by computed tomography is provided. A collimated source of radiation is placed adjacent one side of the wall portion and an array of detectors for the radiation is placed on the other side adjacent the source. The radiation from the source passing through the wall portion is then detected with the detectors over a limited angle, dependent upon the curvature of the wall of the vessel, to obtain a dataset. The source and array are then coordinately moved relative to the wall portion in steps and a further dataset is obtained at each step. The plurality of datasets obtained over the limited angle is then processed to produce a tomogram of the wall portion to determine the presence of a defect therein. In a preferred embodiment, the curved wall portion has a center of curvature so that the source and the array are positioned at each step along a respective arc curved about the center. If desired, the detector array and source can be reoriented relative to a new wall portion and an inspection of the new wall portion can be easily obtained. Further, the source and detector array can be indexed in a direction perpendicular to a plane including the limited angle in a plurality of steps so that by repeating the detecting and moving steps at each index step, a three dimensional image can be created of the wall portion.

Linear sampling method applied to non destructive testing of an elastic waveguide: theory, numerics and experiments

NASA Astrophysics Data System (ADS)

Baronian, Vahan; Bourgeois, Laurent; Chapuis, Bastien; Recoquillay, Arnaud

2018-07-01

This paper presents an application of the linear sampling method to ultrasonic non destructive testing of an elastic waveguide. In particular, the NDT context implies that both the solicitations and the measurements are located on the surface of the waveguide and are given in the time domain. Our strategy consists in using a modal formulation of the linear sampling method at multiple frequencies, such modal formulation being justified theoretically in Bourgeois et al (2011 Inverse Problems 27 055001) for rigid obstacles and in Bourgeois and Lunéville (2013 Inverse Problems 29 025017) for cracks. Our strategy requires the inversion of some emission and reception matrices which deserve some special attention due to potential ill-conditioning. The feasibility of our method is proved with the help of artificial data as well as real data.

PREFACE: III All-Russian Scientific and Practical Conference on Innovations in Non-Destructive Testing (SibTest 2015)

NASA Astrophysics Data System (ADS)

2016-01-01

This issue of the journal is devoted to the research and studies presented at the III All-Russian Scientific and Practical Conference on Innovations in Non-Destructive Testing SibTest. The conference was held in Altai, Russia, on 27-31 July 2015. The conference brought together experts from different countries and organizations who had a great opportunity to share knowledge during oral and poster presentations and to initiate discussions on topics that are of interest to the conference attendees. The conference aimed to discuss innovative methods and the application of advanced technologies in non-destructive testing. The conference also attempted to bring together university, academic and industrial science, to expand the co-operation of scientists from different countries in research and development and the commercialization of innovative technologies in non-destructive testing. The key themes of the conference were: ultrasonic and acoustic testing; electromagnetic and thermal testing; various types of radiation non-destructive testing; passive and active testing techniques. The conference organizers are the Institute of Non-Destructive Testing, Tomsk Polytechnic University, with the assistance of the Russian Society for Non-Destructive Testing and Technical Diagnostics, Institute of Strength Physics and Materials Science, Siberian Branch of the Russian Academy of Sciences, National Research Tomsk State University, Moscow State Institute of Radio Engineering, Electronics and Automation.

Feasibility Study of Non-Destructive Techniques to Measure Corrosion in SAVY Containers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davenport, Matthew Nicholas

2016-07-15

Stainless Steel SAVY containers are used to transport and store nuclear material. They are prone to interior corrosion in the presence of certain chemicals and a low-oxygen environment. SAVY containers also have relatively thin walls to reduce their weight, making their structural integrity more vulnerable to the effects of corrosion. A nondestructive evaluation system that finds and monitors corrosion within containers in use would improve safety conditions and preclude hazards. Non-destructive testing can determine whether oxidation or corrosion is occurring inside the SAVY containers, and there are a variety of non-destructive testing methods that may be viable. The feasibility studymore » described will objectively decide which method best fits the requirements of the facility and the problem. To improve efficiency, the containers cannot be opened during the non-destructive examination. The chosen technique should also be user-friendly and relatively quick to apply. It must also meet facility requirements regarding wireless technology and maintenance. A feasibility study is an objective search for a new technology or product to solve a particular problem. First, the design, technical, and facility feasibility requirements are chosen and ranked in order of importance. Then each technology considered is given a score based upon a standard ranking system. The technology with the highest total score is deemed the best fit for a certain application.« less

Non-destructive techniques for the detection of fungal infection in cereal grains.

PubMed

Orina, Irene; Manley, Marena; Williams, Paul J

2017-10-01

Infection of cereal grains by fungi is a serious problem worldwide. Depending on the environmental conditions, cereal grains may be colonised by different species of fungi. These fungi cause reduction in yield, quality and nutritional value of the grain; and of major concern is their production of mycotoxins which are harmful to both humans and animals. Early detection of fungal contamination is an essential control measure for ensuring storage longevity and food safety. Conventional methods for detection of fungal infection, such as culture and colony techniques or immunological methods are either slow, labour intensive or difficult to automate. In recent years, there has been an increasing need to develop simple, rapid, non-destructive methods for early detection of fungal infection and mycotoxins contamination in cereal grains. Methods such as near infrared (NIR) spectroscopy, NIR hyperspectral imaging, and electronic nose were evaluated for these purposes. This paper reviews the different non-destructive techniques that have been considered thus far for detection of fungal infection and mycotoxins in cereal grains, including their principles, application and limitations. Copyright © 2017 Elsevier Ltd. All rights reserved.

Impedance-based cellular assays for regenerative medicine.

PubMed

Gamal, W; Wu, H; Underwood, I; Jia, J; Smith, S; Bagnaninchi, P O

2018-07-05

Therapies based on regenerative techniques have the potential to radically improve healthcare in the coming years. As a result, there is an emerging need for non-destructive and label-free technologies to assess the quality of engineered tissues and cell-based products prior to their use in the clinic. In parallel, the emerging regenerative medicine industry that aims to produce stem cells and their progeny on a large scale will benefit from moving away from existing destructive biochemical assays towards data-driven automation and control at the industrial scale. Impedance-based cellular assays (IBCA) have emerged as an alternative approach to study stem-cell properties and cumulative studies, reviewed here, have shown their potential to monitor stem-cell renewal, differentiation and maturation. They offer a novel method to non-destructively assess and quality-control stem-cell cultures. In addition, when combined with in vitro disease models they provide complementary insights as label-free phenotypic assays. IBCA provide quantitative and very sensitive results that can easily be automated and up-scaled in multi-well format. When facing the emerging challenge of real-time monitoring of three-dimensional cell culture dielectric spectroscopy and electrical impedance tomography represent viable alternatives to two-dimensional impedance sensing.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'. © 2018 The Author(s).

Improving the Repair Planning System for Mining Equipment on the Basis of Non-destructive Evaluation Data

NASA Astrophysics Data System (ADS)

Drygin, Michael; Kuryshkin, Nicholas

2017-11-01

The article tells about forming a new concept of scheduled preventive repair system of the equipment at coal mining enterprises, based on the use of modem non-destructive evaluation methods. The approach to the solution for this task is based on the system-oriented analysis of the regulatory documentation, non-destructive evaluation methods and means, experimental studies with compilation of statistics and subsequent grapho-analytical analysis. The main result of the work is a feasible explanation of using non-destructive evaluation methods within the current scheduled preventive repair system, their high efficiency and the potential of gradual transition to condition-based maintenance. In practice wide use of nondestructive evaluation means w;ill allow to reduce significantly the number of equipment failures and to repair only the nodes in pre-accident condition. Considering the import phase-out policy, the solution for this task will allow to adapt the SPR system to Russian market economy conditions and give the opportunity of commercial move by reducing the expenses for maintenance of Russian-made and imported equipment.

Quality parameters of mango and potential of non-destructive techniques for their measurement - a review.

PubMed

Jha, S N; Narsaiah, K; Sharma, A D; Singh, M; Bansal, S; Kumar, R

2010-01-01

The king of fruits "Mango" (Mangifera indica L.) is very nutritious and rich in carotenes. India produces about 50% of the total world's mango. Many researchers have reported the maturity indices and quality parameters for determination of harvesting time and eating quality. The methods currently used for determination of quality of mango are mostly based on the biochemical analysis, which leads to destruction of the fruits. Numerous works are being carried out to explore some non-destructive methods such as Near Infrared (NIR), Nuclear Magnetic Resonance (NMR), X-ray and Computed Tomography (CT), electronic nose, machine vision and ultrasound for quality determination of fruits. This paper deals with some recent work reported on quality parameters, harvesting and post-harvest treatments in relation to quality of mango fruits and reviews on some of the potential non-destructive techniques that can be explored for quality determination of mango cultivars.

Improving Non-Destructive Concrete Strength Tests Using Support Vector Machines

PubMed Central

Shih, Yi-Fan; Wang, Yu-Ren; Lin, Kuo-Liang; Chen, Chin-Wen

2015-01-01

Non-destructive testing (NDT) methods are important alternatives when destructive tests are not feasible to examine the in situ concrete properties without damaging the structure. The rebound hammer test and the ultrasonic pulse velocity test are two popular NDT methods to examine the properties of concrete. The rebound of the hammer depends on the hardness of the test specimen and ultrasonic pulse travelling speed is related to density, uniformity, and homogeneity of the specimen. Both of these two methods have been adopted to estimate the concrete compressive strength. Statistical analysis has been implemented to establish the relationship between hammer rebound values/ultrasonic pulse velocities and concrete compressive strength. However, the estimated results can be unreliable. As a result, this research proposes an Artificial Intelligence model using support vector machines (SVMs) for the estimation. Data from 95 cylinder concrete samples are collected to develop and validate the model. The results show that combined NDT methods (also known as SonReb method) yield better estimations than single NDT methods. The results also show that the SVMs model is more accurate than the statistical regression model. PMID:28793627

Automated Non-Destructive Testing Array Evaluation System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wei, T; Zavaljevski, N; Bakhtiari, S

2004-12-24

Automated Non-Destructive Testing Array Evaluation System (ANTARES) sofeware alogrithms were developed for use on X-probe(tm) data. Data used for algorithm development and preliminary perfomance determination was obtained for USNRC mock-up at Argone and data from EPRI.

Non-Destructive Assessment of Residual Strength of Thermally Damaged Concrete Made with Different Aggregate Types

NASA Astrophysics Data System (ADS)

Mróz, Katarzyna; Hager, Izabela

2017-10-01

The paper presents the results obtained for four concretes made with four different aggregate types: basalt, granite, dolomite and riverbed gravel. In this study, the cement paste and mortar compositions and their volumes remained the same for all the four concretes that allow clear comparisons and conclusions of aggregate type effect. Moreover, the aggregate particle size distribution is chosen to be quasi identical for all concretes so that this factor does not affect the concrete behaviour. The residual material properties (after heating and cooling down) are determined with the use of destructive and non-destructive testing methods for each concrete type being not thermally damaged and after thermal exposure at temperature of 200 °C. 400 °C, 600 °C, 800 °C and 1000 °C. Residual mechanical properties are compared with diagnostic parameters obtained with NDT methods. The aim of this study is to provide and compare the regression curves between selected non-destructive diagnostic parameters and the residual values of mechanical properties. The NDT methods used in this experiment are: surface hardness and Ultrasonic Pulse Velocity.

A Distributive, Non-Destructive, Real-Time Approach to Snowpack Monitoring

NASA Technical Reports Server (NTRS)

Frolik, Jeff; Skalka, Christian

2012-01-01

This invention is designed to ascertain the snow water equivalence (SWE) of snowpacks with better spatial and temporal resolutions than present techniques. The approach is ground-based, as opposed to some techniques that are air-based. In addition, the approach is compact, non-destructive, and can be communicated with remotely, and thus can be deployed in areas not possible with current methods. Presently there are two principal ground-based techniques for obtaining SWE measurements. The first is manual snow core measurements of the snowpack. This approach is labor-intensive, destructive, and has poor temporal resolution. The second approach is to deploy a large (e.g., 3x3 m) snowpillow, which requires significant infrastructure, is potentially hazardous [uses a approximately equal to 200-gallon (approximately equal to 760-L) antifreeze-filled bladder], and requires deployment in a large, flat area. High deployment costs necessitate few installations, thus yielding poor spatial resolution of data. Both approaches have limited usefulness in complex and/or avalanche-prone terrains. This approach is compact, non-destructive to the snowpack, provides high temporal resolution data, and due to potential low cost, can be deployed with high spatial resolution. The invention consists of three primary components: a robust wireless network and computing platform designed for harsh climates, new SWE sensing strategies, and algorithms for smart sampling, data logging, and SWE computation.

Non destructive testing of works of art by terahertz analysis

NASA Astrophysics Data System (ADS)

Bodnar, Jean-Luc; Metayer, Jean-Jacques; Mouhoubi, Kamel; Detalle, Vincent

2013-11-01

Improvements in technologies and the growing security needs in airport terminals lead to the development of non destructive testing devices using terahertz waves. Indeed, these waves have the advantage of being, on one hand, relatively penetrating. They also have the asset of not being ionizing. It is thus potentially an interesting contribution in the non destructive testing field. With the help of the VISIOM Company, the possibilities of this new industrial analysis method in assisting the restoration of works of art were then approached. The results obtained within this framework are presented here and compared with those obtained by infrared thermography. The results obtained show first that the THZ method, like the stimulated infrared thermography allows the detection of delamination located in murals paintings or in marquetries. They show then that the THZ method seems to allow detecting defects located relatively deeply (10 mm) and defects potentially concealed by other defects. It is an advantage compared to the stimulated infra-red thermography which does not make it possible to obtain these results. Furthermore, they show that the method does not seem sensitive to the various pigments constituting the pictorial layer, to the presence of a layer of "Japan paper" and to the presence of a layer of whitewash. It is not the case of the stimulated infrared thermography. It is another advantage of the THZ method. Finally, they show that the THZ method is limited in the detection of low-size defects. It is a disadvantage compared to the stimulated infrared thermography.

Non-destructive Faraday imaging of dynamically controlled ultracold atoms

NASA Astrophysics Data System (ADS)

Gajdacz, Miroslav; Pedersen, Poul; Mørch, Troels; Hilliard, Andrew; Arlt, Jan; Sherson, Jacob

2013-05-01

We investigate non-destructive measurements of ultra-cold atomic clouds based on dark field imaging of spatially resolved Faraday rotation. In particular, we pursue applications to dynamically controlled ultracold atoms. The dependence of the Faraday signal on laser detuning, atomic density and temperature is characterized in a detailed comparison with theory. In particular the destructivity per measurement is extremely low and we illustrate this by imaging the same cloud up to 2000 times. The technique is applied to avoid the effect of shot-to-shot fluctuations in atom number calibration. Adding dynamic changes to system parameters, we demonstrate single-run vector magnetic field imaging and single-run spatial imaging of the system's dynamic behavior. The method can be implemented particularly easily in standard imaging systems by the insertion of an extra polarizing beam splitter. These results are steps towards quantum state engineering using feedback control of ultracold atoms.

Evaluation of Electromagnetic Near-Field Measurement Technique as Non-Destructive Testing for Composite Structures

NASA Astrophysics Data System (ADS)

Raad Hussein, Alaa; Badri Albarody, Thar M.; Megat Yusoff, Puteri Sri Melor Bt

2018-05-01

Nowadays there is no viable non-destructive method that could detect flaws in complex composite products. Such a method could provide unique tools to allow engineers to minimize time consumption and cost during the evaluation of various product parameters without disturbing production. The latest research and development on propagation waves introduce micro, radio and millimetre waves as new potential non-destructive test methods for evaluation of mechanical flaws and prediction of failure in a product during production. This paper focuses on recent developments, usage, classification of electromagnetic waves under the range of radio frequency, millimetre and micro-waves. In addition, this paper reviews the application of propagation wave and proposed a new health monitoring technique based on Doppler Effect for vibration measurement in complex composite structures. Doppler Effect is influenced by dynamic behaviour of the composite structures and both are effect by flaws occurred inside the structure. Composite manufacturers, especially Aerospace industry are demanding these methods comprehensively inspect and evaluate the damages and defects in their products.

Non-Destructive Techniques Based on Eddy Current Testing

PubMed Central

García-Martín, Javier; Gómez-Gil, Jaime; Vázquez-Sánchez, Ernesto

2011-01-01

Non-destructive techniques are used widely in the metal industry in order to control the quality of materials. Eddy current testing is one of the most extensively used non-destructive techniques for inspecting electrically conductive materials at very high speeds that does not require any contact between the test piece and the sensor. This paper includes an overview of the fundamentals and main variables of eddy current testing. It also describes the state-of-the-art sensors and modern techniques such as multi-frequency and pulsed systems. Recent advances in complex models towards solving crack-sensor interaction, developments in instrumentation due to advances in electronic devices, and the evolution of data processing suggest that eddy current testing systems will be increasingly used in the future. PMID:22163754

Non-destructive techniques based on eddy current testing.

PubMed

García-Martín, Javier; Gómez-Gil, Jaime; Vázquez-Sánchez, Ernesto

2011-01-01

Non-destructive techniques are used widely in the metal industry in order to control the quality of materials. Eddy current testing is one of the most extensively used non-destructive techniques for inspecting electrically conductive materials at very high speeds that does not require any contact between the test piece and the sensor. This paper includes an overview of the fundamentals and main variables of eddy current testing. It also describes the state-of-the-art sensors and modern techniques such as multi-frequency and pulsed systems. Recent advances in complex models towards solving crack-sensor interaction, developments in instrumentation due to advances in electronic devices, and the evolution of data processing suggest that eddy current testing systems will be increasingly used in the future.

In situ non-destructive measurement of biofilm thickness and topology in an interferometric optical microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larimer, Curtis; Suter, Jonathan D.; Bonheyo, George

Biofilms are ubiquitous and deleteriously impact a wide range of industrial processes, medical and dental health issues, and environmental problems such as transport of invasive species and the fuel efficiency of ocean going vessels. Biofilms are difficult to characterize when fully hydrated, especially in a non-destructive manner, because of their soft structure and water-like bulk properties. Herein we describe a non-destructive high resolution method of measuring and monitoring the thickness and topology of live biofilms of using white light interferometric optical microscopy. Using this technique, surface morphology, surface roughness, and biofilm thickness can be measured non-destructively and with high resolutionmore » as a function of time without disruption of the biofilm activity and processes. The thickness and surface topology of a P. putida biofilm were monitored growing from initial colonization to a mature biofilm. Typical bacterial growth curves were observed. Increase in surface roughness was a leading indicator of biofilm growth.« less

A novel gamma-fitting statistical method for anti-drug antibody assays to establish assay cut points for data with non-normal distribution.

PubMed

Schlain, Brian; Amaravadi, Lakshmi; Donley, Jean; Wickramasekera, Ananda; Bennett, Donald; Subramanyam, Meena

2010-01-31

In recent years there has been growing recognition of the impact of anti-drug or anti-therapeutic antibodies (ADAs, ATAs) on the pharmacokinetic and pharmacodynamic behavior of the drug, which ultimately affects drug exposure and activity. These anti-drug antibodies can also impact safety of the therapeutic by inducing a range of reactions from hypersensitivity to neutralization of the activity of an endogenous protein. Assessments of immunogenicity, therefore, are critically dependent on the bioanalytical method used to test samples, in which a positive versus negative reactivity is determined by a statistically derived cut point based on the distribution of drug naïve samples. For non-normally distributed data, a novel gamma-fitting method for obtaining assay cut points is presented. Non-normal immunogenicity data distributions, which tend to be unimodal and positively skewed, can often be modeled by 3-parameter gamma fits. Under a gamma regime, gamma based cut points were found to be more accurate (closer to their targeted false positive rates) compared to normal or log-normal methods and more precise (smaller standard errors of cut point estimators) compared with the nonparametric percentile method. Under a gamma regime, normal theory based methods for estimating cut points targeting a 5% false positive rate were found in computer simulation experiments to have, on average, false positive rates ranging from 6.2 to 8.3% (or positive biases between +1.2 and +3.3%) with bias decreasing with the magnitude of the gamma shape parameter. The log-normal fits tended, on average, to underestimate false positive rates with negative biases as large a -2.3% with absolute bias decreasing with the shape parameter. These results were consistent with the well known fact that gamma distributions become less skewed and closer to a normal distribution as their shape parameters increase. Inflated false positive rates, especially in a screening assay, shifts the emphasis to confirm

Sensitivity and Calibration of Non-Destructive Evaluation Method That Uses Neural-Net Processing of Characteristic Fringe Patterns

NASA Technical Reports Server (NTRS)

Decker, Arthur J.; Weiland, Kenneth E.

2003-01-01

This paper answers some performance and calibration questions about a non-destructive-evaluation (NDE) procedure that uses artificial neural networks to detect structural damage or other changes from sub-sampled characteristic patterns. The method shows increasing sensitivity as the number of sub-samples increases from 108 to 6912. The sensitivity of this robust NDE method is not affected by noisy excitations of the first vibration mode. A calibration procedure is proposed and demonstrated where the output of a trained net can be correlated with the outputs of the point sensors used for vibration testing. The calibration procedure is based on controlled changes of fastener torques. A heterodyne interferometer is used as a displacement sensor for a demonstration of the challenges to be handled in using standard point sensors for calibration.

Photoacoustic Spectroscopy as a Non-destructive Tool for Quantification of Pesticide Residue in Apple Cuticle

NASA Astrophysics Data System (ADS)

Liu, Lixian; Wang, Yafei; Gao, Chunming; Huan, Huiting; Zhao, Binxing; Yan, Laijun

2015-06-01

Photoacoustic spectroscopy (PAS), the non-destructive method to detect residue of dimethyl-dichloro-vinyl-phosphate (DDVP) pesticide in a cuticle of apple, is described. After constructing the PA experimental setup and identifying three characteristic peaks of DDVP in the near ultraviolet region, the PA spectra of an apple cuticle contaminated with DDVP were collected. The artificial neural network method was then applied to analyze data quantitatively. The results show a correlation coefficient exceeding 0.99 and a detection limit of 0.2 ppm, which is within the national food safety standard for maximum residue limits for pesticides in food (GB 2763-2012). This fact and the non-destructive character of PAS make the approach promising for detection of pesticide residue in fruits.

Non-destructive research methods applied on materials for the new generation of nuclear reactors

NASA Astrophysics Data System (ADS)

Bartošová, I.; Slugeň, V.; Veterníková, J.; Sojak, S.; Petriska, M.; Bouhaddane, A.

2014-06-01

The paper is aimed on non-destructive experimental techniques applied on materials for the new generation of nuclear reactors (GEN IV). With the development of these reactors, also materials have to be developed in order to guarantee high standard properties needed for construction. These properties are high temperature resistance, radiation resistance and resistance to other negative effects. Nevertheless the changes in their mechanical properties should be only minimal. Materials, that fulfil these requirements, are analysed in this work. The ferritic-martensitic (FM) steels and ODS steels are studied in details. Microstructural defects, which can occur in structural materials and can be also accumulated during irradiation due to neutron flux or alpha, beta and gamma radiation, were analysed using different spectroscopic methods as positron annihilation spectroscopy and Barkhausen noise, which were applied for measurements of three different FM steels (T91, P91 and E97) as well as one ODS steel (ODS Eurofer).

Non-destructive control of graphite electrodes with use of current displacement effect

NASA Astrophysics Data System (ADS)

Myatezh, A. V.; Malozyomov, B. V.; Smirnov, M. A.

2017-10-01

The work is devoted to methods of nondestructive diagnostics and their use for solving the problem of diagnosing various defects in solid surface of graphite electrodes used in steelmaking furnaces. Various non-destructive control methods of materials are analyzed. In the article, methods of eddy-current defectoscopy of graphite electrodes are considered. Rationalization of the sensitivity increase of the method and localization of damage is described. Imitating modeling of electromagnetic processes was executed; results were made and conclusions were drawn.

Determination of the Optimum Harvest Window for Apples Using the Non-Destructive Biospeckle Method.

PubMed

Skic, Anna; Szymańska-Chargot, Monika; Kruk, Beata; Chylińska, Monika; Pieczywek, Piotr Mariusz; Kurenda, Andrzej; Zdunek, Artur; Rutkowski, Krzysztof P

2016-05-10

Determination of the optimum harvest window plays a key role in the agro-food chain as the quality of fruit depends on the right harvesting time and appropriate storage conditions during the postharvest period. Usually, indices based on destructive measurements are used for this purpose, like the De Jager Index (PFW-1), FARS index and the most popular Streif Index. In this study, we proposed a biospeckle method for the evaluation of the optimum harvest window (OHW) of the "Ligol" and "Szampion" apple cultivars. The experiment involved eight different maturity stages, of which four were followed by long cold storage and shelf life to assist the determination of the optimum harvest window. The biospeckle activity was studied in relation to standard quality attributes (firmness, acidity, starch, soluble solids content, Streif Index) and physiological parameters (respiration and ethylene emission) of both apple cultivars. Changes of biospeckle activity (BA) over time showed moderate relationships with biochemical changes during apple maturation and ripening. The harvest date suggested by the Streif Index and postharvest quality indicators matched with characteristic decrease in BA. The ability of biospeckle method to characterize the biological state of apples was confirmed by significant correlations of BA with firmness, starch index, total soluble solids and Streif Index, as well as good match with changes in carbon dioxide and ethylene emission. However, it should be noted that correlations between variables changing over time are not as meaningful as independent observations. Also, it is a well-known property of the Pearson's correlation that its value is highly susceptible to outlier data. Due to its non-selective nature the BA reflected only the current biological state of the fruit and could be affected by many other factors. The investigations showed that the optimum harvest window for apples was indicated by the characteristic drop of BA during pre

Determination of the Optimum Harvest Window for Apples Using the Non-Destructive Biospeckle Method

PubMed Central

Skic, Anna; Szymańska-Chargot, Monika; Kruk, Beata; Chylińska, Monika; Pieczywek, Piotr Mariusz; Kurenda, Andrzej; Zdunek, Artur; Rutkowski, Krzysztof P.

2016-01-01

Determination of the optimum harvest window plays a key role in the agro-food chain as the quality of fruit depends on the right harvesting time and appropriate storage conditions during the postharvest period. Usually, indices based on destructive measurements are used for this purpose, like the De Jager Index (PFW-1), FARS index and the most popular Streif Index. In this study, we proposed a biospeckle method for the evaluation of the optimum harvest window (OHW) of the “Ligol” and “Szampion” apple cultivars. The experiment involved eight different maturity stages, of which four were followed by long cold storage and shelf life to assist the determination of the optimum harvest window. The biospeckle activity was studied in relation to standard quality attributes (firmness, acidity, starch, soluble solids content, Streif Index) and physiological parameters (respiration and ethylene emission) of both apple cultivars. Changes of biospeckle activity (BA) over time showed moderate relationships with biochemical changes during apple maturation and ripening. The harvest date suggested by the Streif Index and postharvest quality indicators matched with characteristic decrease in BA. The ability of biospeckle method to characterize the biological state of apples was confirmed by significant correlations of BA with firmness, starch index, total soluble solids and Streif Index, as well as good match with changes in carbon dioxide and ethylene emission. However, it should be noted that correlations between variables changing over time are not as meaningful as independent observations. Also, it is a well-known property of the Pearson’s correlation that its value is highly susceptible to outlier data. Due to its non-selective nature the BA reflected only the current biological state of the fruit and could be affected by many other factors. The investigations showed that the optimum harvest window for apples was indicated by the characteristic drop of BA during pre

Using magnetic levitation for non-destructive quality control of plastic parts.

PubMed

Hennek, Jonathan W; Nemiroski, Alex; Subramaniam, Anand Bala; Bwambok, David K; Yang, Dian; Harburg, Daniel V; Tricard, Simon; Ellerbee, Audrey K; Whitesides, George M

2015-03-04

Magnetic levitation (MagLev) enables rapid and non-destructive quality control of plastic parts. The feasibility of MagLev as a method to: i) rapidly assess injection-molded plastic parts for defects during process optimization, ii) monitor the degradation of plastics after exposure to harsh environmental conditions, and iii) detect counterfeit polymers by density is demonstrated. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Non-destructive monitoring of viability in an ex vivo organ culture model of osteochondral tissue.

PubMed

Elson, K M; Fox, N; Tipper, J L; Kirkham, J; Hall, R M; Fisher, J; Ingham, E

2015-06-30

Organ culture is an increasingly important tool in research, with advantages over monolayer cell culture due to the inherent natural environment of tissues. Successful organ cultures must retain cell viability. The aim of this study was to produce viable and non-viable osteochondral organ cultures, to assess the accumulation of soluble markers in the conditioned medium for predicting tissue viability. Porcine femoral osteochondral plugs were cultured for 20 days, with the addition of Triton X-100 on day 6 (to induce necrosis), camptothecin (to induce apoptosis) or no toxic additives. Tissue viability was assessed by the tissue destructive XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide tetrazolium salt) assay method and LIVE/DEAD® staining of the cartilage at days 0, 6 and 20. Tissue structure was assessed by histological evaluation using haematoxylin & eosin and safranin O. Conditioned medium was assessed every 3-4 days for glucose depletion, and levels of lactate dehydrogenase (LDH), alkaline phosphatase (AP), glycosaminoglycans (GAGs), and matrix metalloproteinase (MMP)-2 and MMP-9. Necrotic cultures immediately showed a reduction in glucose consumption, and an immediate increase in LDH, GAG, MMP-2 and MMP-9 levels. Apoptotic cultures showed a delayed reduction in glucose consumption and delayed increase in LDH, a small rise in MMP-2 and MMP-9, but no significant effect on GAGs released into the conditioned medium. The data showed that tissue viability could be monitored by assessing the conditioned medium for the aforementioned markers, negating the need for tissue destructive assays. Physiologically relevant whole- or part-joint organ culture models, necessary for research and pre-clinical assessment of therapies, could be monitored this way, reducing the need to sacrifice tissues to determine viability, and hence reducing the sample numbers necessary.

Non-destructive Moisture Content Measurement of Bioabsorbable Polymers Used in Medical Implants

NASA Astrophysics Data System (ADS)

Carroll, P. A.; Bell, S. A.; Maxwell, A. S.; Tomlins, P. E.

2012-09-01

Measurements have been made that link moisture content to the degradation of a bioabsorbable polymeric material, poly ( dl-lactide- co-glycolide) (PLGA). Bioabsorbable polymers used in medical implants degrade and are absorbed into the body. In the course of degradation, these polymers absorb water. Progressive non-destructive laboratory measurements of moisture content can be used as a means of tracking changes in these materials over the course of their degradation. Measurements of moisture content were made using a non-destructive microwave resonance instrument. The measurement approach, more usually applied to granular materials, was adapted to measure small, individual solid samples that do not fill the conventional sample volume of the resonator. Using the microwave resonance technique, gains in moisture content were measurable in increasingly degraded samples. The results were confirmed using alternative (destructive) measurements of sample moisture content. The microwave resonance technique offers a non-destructive measurement that can be used to study the degradation characteristics of PLGA. Better understanding of the degradation process can enable the polymer break-down rate to be tailored to match the healing rate of tissue. Non-destructive measurement allows effective study using single rather than multiple samples. This is a strong advantage when novel materials under study may be either expensive or in strictly limited availability.

NonDestructive Evaluation for Industrial & Development Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunter, James F.

2016-10-12

Provide overview of weld inspection for Non-Destructive Testing at LANL. This includes radiography (RT/DR/CR/CT for x-ray & neutron sources), ultrasonic testing (UT/PAUT), dye penetrant inspection (PT), eddy current inspection (ET) and magnetic particle testing (MT). Facilities and capabilities for weld inspection will be summarized with examples.

Non-destructive and non-invasive observation of friction and wear of human joints and of fracture initiation by acoustic emission.

PubMed

Schwalbe, H J; Bamfaste, G; Franke, R P

1999-01-01

Quality control in orthopaedic diagnostics according to DIN EN ISO 9000ff requires methods of non-destructive process control, which do not harm the patient by radiation or by invasive examinations. To obtain an improvement in health economy, quality-controlled and non-destructive measurements have to be introduced into the diagnostics and therapy of human joints and bones. A non-invasive evaluation of the state of wear of human joints and of the cracking tendency of bones is, as of today's point of knowledge, not established. The analysis of acoustic emission signals allows the prediction of bone rupture far below the fracture load. The evaluation of dry and wet bone samples revealed that it is possible to conclude from crack initiation to the bone strength and thus to predict the probability of bone rupture.

Non-destructive forensic latent fingerprint acquisition with chromatic white light sensors

NASA Astrophysics Data System (ADS)

Leich, Marcus; Kiltz, Stefan; Dittmann, Jana; Vielhauer, Claus

2011-02-01

Non-destructive latent fingerprint acquisition is an emerging field of research, which, unlike traditional methods, makes latent fingerprints available for additional verification or further analysis like tests for substance abuse or age estimation. In this paper a series of tests is performed to investigate the overall suitability of a high resolution off-the-shelf chromatic white light sensor for the contact-less and non-destructive latent fingerprint acquisition. Our paper focuses on scanning previously determined regions with exemplary acquisition parameter settings. 3D height field and reflection data of five different latent fingerprints on six different types of surfaces (HDD platter, brushed metal, painted car body (metallic and non-metallic finish), blued metal, veneered plywood) are experimentally studied. Pre-processing is performed by removing low-frequency gradients. The quality of the results is assessed subjectively; no automated feature extraction is performed. Additionally, the degradation of the fingerprint during the acquisition period is observed. While the quality of the acquired data is highly dependent on surface structure, the sensor is capable of detecting the fingerprint on all sample surfaces. On blued metal the residual material is detected; however, the ridge line structure dissolves within minutes after fingerprint placement.

Non-destructive scanning for applied stress by the continuous magnetic Barkhausen noise method

NASA Astrophysics Data System (ADS)

Franco Grijalba, Freddy A.; Padovese, L. R.

2018-01-01

This paper reports the use of a non-destructive continuous magnetic Barkhausen noise technique to detect applied stress on steel surfaces. The stress profile generated in a sample of 1070 steel subjected to a three-point bending test is analyzed. The influence of different parameters such as pickup coil type, scanner speed, applied magnetic field and frequency band analyzed on the effectiveness of the technique is investigated. A moving smoothing window based on a second-order statistical moment is used to analyze the time signal. The findings show that the technique can be used to detect applied stress profiles.

Non-destructive evaluation of ripening and quality traits in apples using a multiparametric fluorescence sensor.

PubMed

Betemps, Débora L; Fachinello, José Carlos; Galarça, Simone P; Portela, Nicácia M; Remorini, Damiano; Massai, Rossano; Agati, Giovanni

2012-07-01

The detection of pigments and colourless flavonoids in apples can provide a useful indication of fruit quality. Optical methods are preferable because they are fast and non-destructive. In this study, a fluorescence-based portable sensor was used in order to non-invasively determine the content of chlorophylls, anthocyanins and flavonols in Fuji, Granny Smith and Golden Delicious apple cultivars. The aim was to define new non-destructive optical indices of apple quality. The anthocyanin index (ANTH) in Fuji was higher in the sunny (i.e. sun-exposed) side of the fruit compared to the shady side. For all cultivars, the flavonol index (FLAV) was higher in the sunny side compared with the shady side. The chlorophyll index (CHL) for the shady sides of Granny Smith and Golden Delicious was significantly higher than for the sunny sides. Fine linear regressions were found between the ANTH, FLAV and CHL indices and the actual anthocyanin, flavonol and chlorophyll concentrations, respectively, which were determined destructively on the apple peel extracts. A negative correlation was found between the apple sugar content and the chlorophyll fluorescence in the far-red spectral band. Our results indicate that a single multiparametric fluorescence-based sensor can provide valuable non-destructive markers of ripening and quality in apples. Copyright © 2012 Society of Chemical Industry.

NON-DESTRUCTIVE SOIL CARBON ANALYZER.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wielopolski, Lucian; Hendrey, G.; Orion, I.

2004-02-01

This report describes the feasibility, calibration, and safety considerations of a non-destructive, in situ, quantitative, volumetric soil carbon analytical method based on inelastic neutron scattering (INS). The method can quantify values as low as 0.018 gC/cc, or about 1.2% carbon by weight with high precision under the instrument's configuration and operating conditions reported here. INS is safe and easy to use, residual soil activation declines to background values in under an hour, and no radiological requirements are needed for transporting the instrument. The labor required to obtain soil-carbon data is about 10-fold less than with other methods, and the instrumentmore » offers a nearly instantaneous rate of output of carbon-content values. Furthermore, it has the potential to quantify other elements, particularly nitrogen. New instrumentation was developed in response to a research solicitation from the U.S. Department of Energy (DOE LAB 00-09 Carbon Sequestration Research Program) supporting the Terrestrial Carbon Processes (TCP) program of the Office of Science, Biological and Environmental Research (BER). The solicitation called for developing and demonstrating novel techniques for quantitatively measuring changes in soil carbon. The report includes raw data and analyses of a set of proof-of-concept, double-blind studies to evaluate the INS approach in the first phase of developing the instrument. Managing soils so that they sequester massive amounts of carbon was suggested as a means to mitigate the atmospheric buildup of anthropogenic CO{sub 2}. Quantifying changes in the soils' carbon stocks will be essential to evaluating such schemes and documenting their performance. Current methods for quantifying carbon in soil by excavation and core sampling are invasive, slow, labor-intensive and locally destroy the system being observed. Newly emerging technologies, such as Laser Induced Breakdown Spectroscopy and Near-Infrared Spectroscopy, offer soil

Potential applicability of stress wave velocity method on pavement base materials as a non-destructive testing technique

NASA Astrophysics Data System (ADS)

Mahedi, Masrur

Aggregates derived from natural sources have been used traditionally as the pavement base materials. But in recent times, the extraction of these natural aggregates has become more labor intensive and costly due to resource depletion and environmental concerns. Thus, the uses of recycled aggregates as the supplementary of natural aggregates are increasing considerably in pavement construction. Use of recycled aggregates such as recycled crushed concrete (RCA) and recycled asphalt pavement (RAP) reduces the rate of natural resource depletion, construction debris and cost. Although recycled aggregates could be used as a viable alternative of conventional base materials, strength characteristics and product variability limit their utility to a great extent. Hence, their applicability is needed to be evaluated extensively based on strength, stiffness and cost factors. But for extensive evaluation, traditionally practiced test methods are proven to be unreasonable in terms of time, cost, reliability and applicability. On the other hand, rapid non-destructive methods have the potential to be less time consuming and inexpensive along with the low variability of test results; therefore improving the reliability of estimated performance of the pavement. In this research work, the experimental program was designed to assess the potential application of stress wave velocity method as a non-destructive test in evaluating recycled base materials. Different combinations of cement treated recycled concrete aggregate (RAP) and recycled crushed concrete (RCA) were used to evaluate the applicability of stress wave velocity method. It was found that, stress wave velocity method is excellent in characterizing the strength and stiffness properties of cement treated base materials. Statistical models, based on P-wave velocity were derived for predicting the modulus of elasticity and compressive strength of different combinations of cement treated RAP, Grade-1 and Grade-2 materials. Two

Applications of Non-destructive methods (GPR and 3D Laser Scanner) in Historic Masonry Arch Bridge Assessment

NASA Astrophysics Data System (ADS)

Alani, Amir; Banks, Kevin

2014-05-01

There exist approximately 70,000 masonry arch bridge spans (brick and stone) in the UK with tens of thousands more throughout Europe. A significant number of these bridges are still in operation and form part of the road and rail network systems in many countries. A great majority of these bridges are in desperate need of repair and maintenance. Applications of non-destructive testing methods such as ground penetrating radar (GPR), 3D laser scanning, accelerometer sensors and vibration detecting sensors amongst many others have been used to assess and monitor such structures in the past few years. This presentation provides results of the applications of a 2GHz GPR antenna system and a 3D laser scanner on a historic masonry arch bridge (the Old Bridge, Aylesford) located in Kent, in the south east of England. The older part of the bridge (the mid-span) is 860 years old. The bridge was the subject of a major alteration in 1811. This presentation forms part of a larger ongoing study which is using the two above mentioned non-destructive methods for long-term monitoring of the bridge. The adopted survey planning strategy and technique, data acquisition and processing as well as challenges encountered during actual survey and fieldworks have been discussed in this presentation. As a result of this study the position of different layers of the deck structure has been established with the identification of the original stone base of the bridge. This information in addition to the location of a number of structural ties (anchors - remedial work carried out previously) in the absence of reliable and accurate design details proved to be extremely useful for the modelling of the bridge using the finite element method. Results of the 3D laser scanning of the bridge have also been presented which have provided invaluable data essential for the accurate modelling of the bridge as well as the long term monitoring of the bridge. 2014 EGU-GA GI3.1 Session, organised by COST Action

Adhesive Defect Monitoring of Glass Fiber Epoxy Plate Using an Impedance-Based Non-Destructive Testing Method for Multiple Structures

PubMed Central

Na, Wongi S.; Baek, Jongdae

2017-01-01

The emergence of composite materials has revolutionized the approach to building engineering structures. With the number of applications for composites increasing every day, maintaining structural integrity is of utmost importance. For composites, adhesive bonding is usually the preferred choice over the mechanical fastening method, and monitoring for delamination is an essential factor in the field of composite materials. In this study, a non-destructive method known as the electromechanical impedance method is used with an approach of monitoring multiple areas by specifying certain frequency ranges to correspond to a certain test specimen. Experiments are conducted using various numbers of stacks created by attaching glass fiber epoxy composite plates onto one another, and two different debonding damage types are introduced to evaluate the performance of the multiple monitoring electromechanical impedance method. PMID:28629194

Infrared Thermography for Temperature Measurement and Non-Destructive Testing

PubMed Central

Usamentiaga, Rubèn; Venegas, Pablo; Guerediaga, Jon; Vega, Laura; Molleda, Julio; Bulnes, Francisco G.

2014-01-01

The intensity of the infrared radiation emitted by objects is mainly a function of their temperature. In infrared thermography, this feature is used for multiple purposes: as a health indicator in medical applications, as a sign of malfunction in mechanical and electrical maintenance or as an indicator of heat loss in buildings. This paper presents a review of infrared thermography especially focused on two applications: temperature measurement and non-destructive testing, two of the main fields where infrared thermography-based sensors are used. A general introduction to infrared thermography and the common procedures for temperature measurement and non-destructive testing are presented. Furthermore, developments in these fields and recent advances are reviewed. PMID:25014096

The application of non-destructive methods in the diagnostics of the approach pavement at the bridges

NASA Astrophysics Data System (ADS)

Miskiewicz, M.; Lachowicz, J.; Tysiac, P.; Jaskula, P.; Wilde, K.

2018-05-01

The article presents the possibility of using non-destructive methods of road pavement diagnostics as an alternative to traditional means to assess the reasons for premature cracks adjacent to bridge objects. Two scanning methods were used: laser scanning to measure geometric surface deformation and ground penetrating radar (GPR) inspection to assess the road pavement condition. With the use of a laser scanner, an effective tool for road deformation assessment several approach pavement surfaces next to the bridges were scanned. As the result, a point cloud was obtained including spatial information about the pavement deformation. The data accuracy was about 3 mm, the deformations were presented in the form of deviation maps between the reference surface and the actual surface. Moreover characteristic pavement surface cross-sections were presented. The in situ measurements of the GPR method were performed and analysed in order to detect non-homogeneity in the density of structural layers of the pavement. Due to the analysis of the permittivity of individual layers, it was possible to detect non-homogeneity areas. The performed GPR measurements were verified by standard invasive tests carried out by drilling boreholes and taking cores from the pavement and testing the compaction and air voids content in asphalt layers. As a result of the measurements made by both methods significant differences in layer compacting factor values were diagnosed. The factor was much smaller in the area directly next to the bridgehead and much larger in the zone located a few meters away. The research showed the occurrence of both design and erection errors as well as those related to the maintenance of engineering structures.

Innovative real-time and non-destructive method of beam profile measurement under large beam current irradiation for BNCT

NASA Astrophysics Data System (ADS)

Takada, M.; Kamada, S.; Suda, M.; Fujii, R.; Nakamura, M.; Hoshi, M.; Sato, H.; Endo, S.; Hamano, T.; Arai, S.; Higashimata, A.

2012-10-01

We developed a real-time and non-destructive method of beam profile measurement on a target under large beam current irradiation, and without any complex radiation detectors or electrical circuits. We measured the beam profiles on a target by observing the target temperature using an infrared-radiation thermometer camera. The target temperatures were increased and decreased quickly by starting and stopping the beam irradiation within 1 s in response speed. Our method could trace beam movements rapidly. The beam size and position were calibrated by measuring O-ring heat on the target. Our method has the potential to measure beam profiles at beam current over 1 mA for proton and deuteron with the energy around 3 MeV and allows accelerator operators to adjust the beam location during beam irradiation experiments without decreasing the beam current.

Non-destructive in-situ method and apparatus for determining radionuclide depth in media

DOEpatents

Xu, X. George; Naessens, Edward P.

2003-01-01

A non-destructive method and apparatus which is based on in-situ gamma spectroscopy is used to determine the depth of radiological contamination in media such as concrete. An algorithm, Gamma Penetration Depth Unfolding Algorithm (GPDUA), uses point kernel techniques to predict the depth of contamination based on the results of uncollided peak information from the in-situ gamma spectroscopy. The invention is better, faster, safer, and/cheaper than the current practice in decontamination and decommissioning of facilities that are slow, rough and unsafe. The invention uses a priori knowledge of the contaminant source distribution. The applicable radiological contaminants of interest are any isotopes that emit two or more gamma rays per disintegration or isotopes that emit a single gamma ray but have gamma-emitting progeny in secular equilibrium with its parent (e.g., .sup.60 Co, .sup.235 U, and .sup.137 Cs to name a few). The predicted depths from the GPDUA algorithm using Monte Carlo N-Particle Transport Code (MCNP) simulations and laboratory experiments using .sup.60 Co have consistently produced predicted depths within 20% of the actual or known depth.

Research on Non-Destructive Testing Technology in Conservation Repair Project of Ancestral Temple in Mukden Palace

NASA Astrophysics Data System (ADS)

Yang, J.; Fu, M.

2017-08-01

Due to the use of wood and other non-permanent materials, traditional Chinese architecture is one of the most fragile constructions in various heritage objects today. With the increasing emphasis on the protection of cultural relics, the repair project of wooden structure has become more and more important. There are various kinds of destructions, which pose a hidden danger to the overall safety of the ancient buildings, caused not only by time and nature, but also by improper repairs in history or nowadays. Today, the use of digital technology is a basic requirement in the conservation of cultural heritage. Detection technology, especially non-destructive testing technology, could provide more accurate records in capturing detailed physical characteristics of structures such as geometric deformation and invisible damage, as well as prevent a man-made destruction in the process of repair project. This paper aims to interpret with a typical example, Ancestral Temple in Mukden Palace, along with a discussion of how to use the non-destructive testing technology with ground penetrating radar, stress wave, resistograph and so on, in addition to find an appropriate protection method in repair project of traditional Chinese wooden architecture.

Review of the Air-Coupled Impact-Echo Method for Non-Destructive Testing

NASA Astrophysics Data System (ADS)

Nowotarski, Piotr; Dubas, Sebastian; Milwicz, Roman

2017-10-01

The article presents the general idea of Air-Coupled Impact-Echo (ACIE) method which is one of the non-destructive testing (NDT) techniques used in the construction industry. One of the main advantages of the general Impact Echo (IE) method is that it is sufficient to access from one side to that of the structure which greatly facilitate research in the road facilities or places which are difficult to access and diagnose. The main purpose of the article is to present state-of-the-art related to ACIE method based on the publications available at Thomson Reuters Web of Science Core Collection database (WOS) with the further analysis of the mentioned methods. Deeper analysis was also performed for the newest publications published within last 3 years related to ACIE for investigation on the subject of main focus of the researchers and scientists to try to define possible regions where additional examination and work is necessary. One of the main conclusions that comes from the performed analysis is that ACIE methods can be widely used for performing NDT of concrete structures and can be performed faster than standard IE method thanks to the Air-coupled sensors. What is more, 92.3% of the analysed recent research described in publications connected with ACIE was performed in laboratories, and only 23.1% in-situ on real structures. This indicates that method requires further research to prepare test stand ready to perform analysis on real objects outside laboratory conditions. Moreover, algorithms that are used for data processing and later presentation in ACIE method are still being developed and there is no universal solution available for all kinds of the existing and possible to find defects, which indicates possible research area for further works. Authors are of the opinion that emerging ACIE method could be good opportunity for ND testing especially for concrete structures. Development and refinement of test stands that will allow to perform in-situ tests could

Are those bugs reflective? Non-destructive biofilm imaging with white light interferometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larimer, Curtis J.; Brann, Michelle R.; Suter, Jonathan D.

White light interferometry (WLI) is not typically used to image bacterial biofilms that are immersed in water because there is insufficient refractive index contrast to induce reflection from the biofilm’s interface. The soft structure and water-like bulk properties of hydrated biofilms make them difficult to characterize in situ by any means, especially in a non-destructive manner. Here we describe a new method for measuring and monitoring the thickness and topology of live biofilms using a WLI microscope. A microfluidic system was used to create a reflective interface on the surface of biofilms. Live biofilm samples were monitored non-destructively over time.more » The method enables surface metrology measurements (roughness, surface area) and a novel approach to measuring thickness of the thin hydrated biofilms. Increase in surface roughness preceded observable increase in biofilm thickness, indicating that this measure may be used to predict future development of biofilms. We have also developed a flow cell that enables WLI biofilm imaging in a dynamic environment. We have used this flow cell to observe changes in biofilm structure in response to changes in environmental conditions - flow velocity, availability of nutrients, and presence of biocides.« less

Non-destructive testing (NDT) of metal cracks using a high Tc rf-SQUID and eddy current method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, D.F.; Fan, C.; Ruan, J.Z.

1994-12-31

A SQUID is the most sensitive device to detect change in magnetic field. A non-destructive testing (NDT) device using high temperature SQUIDs and eddy current method will be much more sensitive than those currently used eddy current systems, yet much cheaper than one with low temperature SQUIDs. In this paper, we present our study of such a NDT device using a high temperature superconducting rf-SQUID as a gradiometer sensor. The result clearly demonstrates the expected sensitivity of the system, and indicates the feasibility of building a portable HTS SQUID NDT device with the help from cryocooler industry. Such a NDTmore » device will have a significant impact on metal corrosion or crack detection technology.« less

The use of fractional order derivatives for eddy current non-destructive testing

NASA Astrophysics Data System (ADS)

Sikora, Ryszard; Grzywacz, Bogdan; Chady, Tomasz

2018-04-01

The paper presents the possibility of using the fractional derivatives for non-destructive testing when a multi-frequency method based on eddy current is applied. It is shown that frequency characteristics obtained during tests can be approximated by characteristics of a proposed model in the form of fractional order transfer function, and values of parameters of this model can be utilized for detection and identification of defects.

A novel combined approach of diffuse reflectance UV-Vis-NIR spectroscopy and multivariate analysis for non-destructive examination of blue ballpoint pen inks in forensic application

NASA Astrophysics Data System (ADS)

Kumar, Raj; Sharma, Vishal

2017-03-01

The present research is focused on the analysis of writing inks using destructive UV-Vis spectroscopy (dissolution of ink by the solvent) and non-destructive diffuse reflectance UV-Vis-NIR spectroscopy along with Chemometrics. Fifty seven samples of blue ballpoint pen inks were analyzed under optimum conditions to determine the differences in spectral features of inks among same and different manufacturers. Normalization was performed on the spectroscopic data before chemometric analysis. Principal Component Analysis (PCA) and K-mean cluster analysis were used on the data to ascertain whether the blue ballpoint pen inks could be differentiated by their UV-Vis/UV-Vis NIR spectra. The discriminating power is calculated by qualitative analysis by the visual comparison of the spectra (absorbance peaks), produced by the destructive and non-destructive methods. In the latter two methods, the pairwise comparison is made by incorporating the clustering method. It is found that chemometric method provides better discriminating power (98.72% and 99.46%, in destructive and non-destructive, respectively) in comparison to the qualitative analysis (69.67%).

A novel combined approach of diffuse reflectance UV-Vis-NIR spectroscopy and multivariate analysis for non-destructive examination of blue ballpoint pen inks in forensic application.

PubMed

Kumar, Raj; Sharma, Vishal

2017-03-15

The present research is focused on the analysis of writing inks using destructive UV-Vis spectroscopy (dissolution of ink by the solvent) and non-destructive diffuse reflectance UV-Vis-NIR spectroscopy along with Chemometrics. Fifty seven samples of blue ballpoint pen inks were analyzed under optimum conditions to determine the differences in spectral features of inks among same and different manufacturers. Normalization was performed on the spectroscopic data before chemometric analysis. Principal Component Analysis (PCA) and K-mean cluster analysis were used on the data to ascertain whether the blue ballpoint pen inks could be differentiated by their UV-Vis/UV-Vis NIR spectra. The discriminating power is calculated by qualitative analysis by the visual comparison of the spectra (absorbance peaks), produced by the destructive and non-destructive methods. In the latter two methods, the pairwise comparison is made by incorporating the clustering method. It is found that chemometric method provides better discriminating power (98.72% and 99.46%, in destructive and non-destructive, respectively) in comparison to the qualitative analysis (69.67%). Copyright © 2016 Elsevier B.V. All rights reserved.

39 CFR 762.41 - Advice of non-receipt or loss, destruction, or mutilation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 39 Postal Service 1 2011-07-01 2011-07-01 false Advice of non-receipt or loss, destruction, or..., Mutilated, and Defaced Disbursement Postal Money Orders § 762.41 Advice of non-receipt or loss, destruction... purpose for which it was issued, giving, if possible, its date, number, and amount, and requesting that...

39 CFR 762.41 - Advice of non-receipt or loss, destruction, or mutilation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 39 Postal Service 1 2010-07-01 2010-07-01 false Advice of non-receipt or loss, destruction, or..., Mutilated, and Defaced Disbursement Postal Money Orders § 762.41 Advice of non-receipt or loss, destruction... purpose for which it was issued, giving, if possible, its date, number, and amount, and requesting that...

Influence of Kaolin in Fly Ash Based Geopolymer Concrete: Destructive and Non-Destructive Testing

NASA Astrophysics Data System (ADS)

Yahya, Z.; Abdullah, M. M. A. B.; Ramli, N. Mohd; Burduhos-Nergis, D. D.; Razak, R. Abd

2018-06-01

Development of geopolymer concrete is mainly to reduce the production of ordinary Portland cement (OPC) that adverse the natural effect. Fly ash is a by-product collected from electrical generating power plant which resulted from burning pulverized coal. Since fly ash is waste materials, it can be recycled for future advantages particularly as pozzolanic materials in construction industry. This study focused on the feasibility of fly ash based geopolymer concrete to which kaolin has been added. The main constituents of geopolymer production for this study were class F fly ash, sodium silicate and sodium hydroxide (NaOH) solution. The concentration of NaOH solution was fixed at 12 Molar, ratio of fly ash/alkaline activator and sodium silicate/NaOH fixed at 1.5 and 2.5, respectively. Kaolin was added in range 5% to 15% from the mass of fly ash and all the samples were cured at room temperature. Destructive and non-destructive test were performed on geopolymer concrete to evaluate the best mix proportions that yield the highest strength as well as the quality of the concrete. Compressive strength, flexural strength, rebound hammer and ultrasonic pulse velocity (UPV) result have been obtained. It shown that 5% replacement of kaolin contributed to maximum compressive strength and flexural strength of 40.4 MPa and 12.35 MPa at 28 days. These result was supported by non-destructive test for the same mix proportion.

Californium interrogation prompt neutron (CIPN) instrument for non-destructive assay of spent nuclear fuel – design concept and experimental demonstration

DOE PAGES

Henzlova, Daniela; Menlove, Howard Olsen; Rael, Carlos D.; ...

2015-10-09

Our paper presents results of the first experimental demonstration of the Californium Interrogation Prompt Neutron (CIPN) instrument developed within a multi-year effort launched by the Next Generation Safeguards Initiative Spent Fuel Project of the United States Department of Energy. The goals of this project focused on developing viable non-destructive assay techniques with capabilities to improve an independent verification of spent fuel assembly characteristics. For this purpose, the CIPN instrument combines active and passive neutron interrogation, along with passive gamma-ray measurements, to provide three independent observables. We describe the initial feasibility demonstration of the CIPN instrument, which involved measurements of fourmore » pressurized-water-reactor spent fuel assemblies with different levels of burnup and two initial enrichments. The measurements were performed at the Post-Irradiation Examination Facility at the Korea Atomic Energy Institute in the Republic of Korea. The key aim of the demonstration was to evaluate CIPN instrument performance under realistic deployment conditions, with the focus on a detailed assessment of systematic uncertainties that are best evaluated experimentally. The measurements revealed good positioning reproducibility, as well as a high degree of insensitivity of the CIPN instrument's response to irregularities in a radial burnup profile. Systematic uncertainty of individual CIPN instrument signals due to assembly rotation was found to be <4.5%, even for assemblies with fairly extreme gradients in the radial burnup profile. Lastly, these features suggest that the CIPN instrument is capable of providing a good representation of assembly average characteristics, independent of assembly orientation in the instrument.« less

Californium interrogation prompt neutron (CIPN) instrument for non-destructive assay of spent nuclear fuel – design concept and experimental demonstration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henzlova, Daniela; Menlove, Howard Olsen; Rael, Carlos D.

Our paper presents results of the first experimental demonstration of the Californium Interrogation Prompt Neutron (CIPN) instrument developed within a multi-year effort launched by the Next Generation Safeguards Initiative Spent Fuel Project of the United States Department of Energy. The goals of this project focused on developing viable non-destructive assay techniques with capabilities to improve an independent verification of spent fuel assembly characteristics. For this purpose, the CIPN instrument combines active and passive neutron interrogation, along with passive gamma-ray measurements, to provide three independent observables. We describe the initial feasibility demonstration of the CIPN instrument, which involved measurements of fourmore » pressurized-water-reactor spent fuel assemblies with different levels of burnup and two initial enrichments. The measurements were performed at the Post-Irradiation Examination Facility at the Korea Atomic Energy Institute in the Republic of Korea. The key aim of the demonstration was to evaluate CIPN instrument performance under realistic deployment conditions, with the focus on a detailed assessment of systematic uncertainties that are best evaluated experimentally. The measurements revealed good positioning reproducibility, as well as a high degree of insensitivity of the CIPN instrument's response to irregularities in a radial burnup profile. Systematic uncertainty of individual CIPN instrument signals due to assembly rotation was found to be <4.5%, even for assemblies with fairly extreme gradients in the radial burnup profile. Lastly, these features suggest that the CIPN instrument is capable of providing a good representation of assembly average characteristics, independent of assembly orientation in the instrument.« less

Non-destructive evaluation of UV pulse laser-induced damage performance of fused silica optics.

PubMed

Huang, Jin; Wang, Fengrui; Liu, Hongjie; Geng, Feng; Jiang, Xiaodong; Sun, Laixi; Ye, Xin; Li, Qingzhi; Wu, Weidong; Zheng, Wanguo; Sun, Dunlu

2017-11-24

The surface laser damage performance of fused silica optics is related to the distribution of surface defects. In this study, we used chemical etching assisted by ultrasound and magnetorheological finishing to modify defect distribution in a fused silica surface, resulting in fused silica samples with different laser damage performance. Non-destructive test methods such as UV laser-induced fluorescence imaging and photo-thermal deflection were used to characterize the surface defects that contribute to the absorption of UV laser radiation. Our results indicate that the two methods can quantitatively distinguish differences in the distribution of absorptive defects in fused silica samples subjected to different post-processing steps. The percentage of fluorescence defects and the weak absorption coefficient were strongly related to the damage threshold and damage density of fused silica optics, as confirmed by the correlation curves built from statistical analysis of experimental data. The results show that non-destructive evaluation methods such as laser-induced fluorescence and photo-thermal absorption can be effectively applied to estimate the damage performance of fused silica optics at 351 nm pulse laser radiation. This indirect evaluation method is effective for laser damage performance assessment of fused silica optics prior to utilization.

Non-destructive single-pass low-noise detection of ions in a beamline

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmidt, Stefan; Institut für Kernchemie, Johannes Gutenberg–Universität Mainz, 55099 Mainz; Murböck, Tobias

2015-11-15

We have conceived, built, and operated a device for the non-destructive single-pass detection of charged particles in a beamline. The detector is based on the non-resonant pick-up and subsequent low-noise amplification of the image charges induced in a cylindrical electrode surrounding the particles’ beam path. The first stage of the amplification electronics is designed to be operated from room temperature down to liquid helium temperature. The device represents a non-destructive charge counter as well as a sensitive timing circuit. We present the concept and design details of the device. We have characterized its performance and show measurements with low-energy highlymore » charged ions (such as Ar{sup 13+}) passing through one of the electrodes of a cylindrical Penning trap. This work demonstrates a novel approach of non-destructive, low noise detection of charged particles which is, depending on the bunch structure, suitable, e.g., for ion traps, low-energy beamlines or accelerator transfer sections.« less

Non-destructive mapping of grain orientations in 3D by laboratory X-ray microscopy

NASA Astrophysics Data System (ADS)

McDonald, S. A.; Reischig, P.; Holzner, C.; Lauridsen, E. M.; Withers, P. J.; Merkle, A. P.; Feser, M.

2015-10-01

The ability to characterise crystallographic microstructure, non-destructively and in three-dimensions, is a powerful tool for understanding many aspects related to damage and deformation mechanisms in polycrystalline materials. To this end, the technique of X-ray diffraction contrast tomography (DCT) using monochromatic synchrotron and polychromatic laboratory X-ray sources has been shown to be capable of mapping crystal grains and their orientations non-destructively in 3D. Here we describe a novel laboratory-based X-ray DCT modality (LabDCT), enabling the wider accessibility of the DCT technique for routine use and in-depth studies of, for example, temporal changes in crystallographic grain structure non-destructively over time through ‘4D’ in situ time-lapse studies. The capability of the technique is demonstrated by studying a titanium alloy (Ti-β21S) sample. In the current implementation the smallest grains that can be reliably detected are around 40 μm. The individual grain locations and orientations are reconstructed using the LabDCT method and the results are validated against independent measurements from phase contrast tomography and electron backscatter diffraction respectively. Application of the technique promises to provide important insights related to the roles of recrystallization and grain growth on materials properties as well as supporting 3D polycrystalline modelling of materials performance.

Non-destructive mapping of grain orientations in 3D by laboratory X-ray microscopy.

PubMed

McDonald, S A; Reischig, P; Holzner, C; Lauridsen, E M; Withers, P J; Merkle, A P; Feser, M

2015-10-23

The ability to characterise crystallographic microstructure, non-destructively and in three-dimensions, is a powerful tool for understanding many aspects related to damage and deformation mechanisms in polycrystalline materials. To this end, the technique of X-ray diffraction contrast tomography (DCT) using monochromatic synchrotron and polychromatic laboratory X-ray sources has been shown to be capable of mapping crystal grains and their orientations non-destructively in 3D. Here we describe a novel laboratory-based X-ray DCT modality (LabDCT), enabling the wider accessibility of the DCT technique for routine use and in-depth studies of, for example, temporal changes in crystallographic grain structure non-destructively over time through '4D' in situ time-lapse studies. The capability of the technique is demonstrated by studying a titanium alloy (Ti-β21S) sample. In the current implementation the smallest grains that can be reliably detected are around 40 μm. The individual grain locations and orientations are reconstructed using the LabDCT method and the results are validated against independent measurements from phase contrast tomography and electron backscatter diffraction respectively. Application of the technique promises to provide important insights related to the roles of recrystallization and grain growth on materials properties as well as supporting 3D polycrystalline modelling of materials performance.

Towards Large-Scale, Non-Destructive Inspection of Concrete Bridges

NASA Astrophysics Data System (ADS)

Mahmoud, A.; Shah, A. H.; Popplewell, N.

2005-04-01

It is estimated that the rehabilitation of deteriorating engineering infrastructure in the harsh North American environment could cost billions of dollars. Bridges are key infrastructure components for surface transportation. Steel-free and fibre-reinforced concrete is used increasingly nowadays to circumvent the vulnerability of steel rebar to corrosion. Existing steel-free and fibre-reinforced bridges may experience extensive surface-breaking cracks that need to be characterized without incurring further damage. In the present study, a method that uses Lamb elastic wave propagation to non-destructively characterize cracks in plain as well as fibre-reinforced concrete is investigated both numerically and experimentally. Numerical and experimental data are corroborated with good agreement.

Non-Destructive Thermography Analysis of Impact Damage on Large-Scale CFRP Automotive Parts

PubMed Central

Maier, Alexander; Schmidt, Roland; Oswald-Tranta, Beate; Schledjewski, Ralf

2014-01-01

Laminated composites are increasingly used in aeronautics and the wind energy industry, as well as in the automotive industry. In these applications, the construction and processing need to fulfill the highest requirements regarding weight and mechanical properties. Environmental issues, like fuel consumption and CO2-footprint, set new challenges in producing lightweight parts that meet the highly monitored standards for these branches. In the automotive industry, one main aspect of construction is the impact behavior of structural parts. To verify the quality of parts made from composite materials with little effort, cost and time, non-destructive test methods are increasingly used. A highly recommended non-destructive testing method is thermography analysis. In this work, a prototype for a car’s base plate was produced by using vacuum infusion. For research work, testing specimens were produced with the same multi-layer build up as the prototypes. These specimens were charged with defined loads in impact tests to simulate the effect of stone chips. Afterwards, the impacted specimens were investigated with thermography analysis. The research results in that work will help to understand the possible fields of application and the usage of thermography analysis as the first quick and economic failure detection method for automotive parts. PMID:28788464

Non-Destructive Thermography Analysis of Impact Damage on Large-Scale CFRP Automotive Parts.

PubMed

Maier, Alexander; Schmidt, Roland; Oswald-Tranta, Beate; Schledjewski, Ralf

2014-01-14

Laminated composites are increasingly used in aeronautics and the wind energy industry, as well as in the automotive industry. In these applications, the construction and processing need to fulfill the highest requirements regarding weight and mechanical properties. Environmental issues, like fuel consumption and CO₂-footprint, set new challenges in producing lightweight parts that meet the highly monitored standards for these branches. In the automotive industry, one main aspect of construction is the impact behavior of structural parts. To verify the quality of parts made from composite materials with little effort, cost and time, non-destructive test methods are increasingly used. A highly recommended non-destructive testing method is thermography analysis. In this work, a prototype for a car's base plate was produced by using vacuum infusion. For research work, testing specimens were produced with the same multi-layer build up as the prototypes. These specimens were charged with defined loads in impact tests to simulate the effect of stone chips. Afterwards, the impacted specimens were investigated with thermography analysis. The research results in that work will help to understand the possible fields of application and the usage of thermography analysis as the first quick and economic failure detection method for automotive parts.

Detection of Secondary Phases in UNS S32760 Superduplex Stainless Steel by Destructive and Non-destructive Techniques

NASA Astrophysics Data System (ADS)

Argandona, G.; Biezma, M. V.; Berrueta, J. M.; Berlanga, C.; Ruiz, A.

2016-12-01

Duplex stainless steels (DSS), with a microstructure of an approximately equal mixture of ferrite ( α) and austenite ( γ) phases, are susceptible to the formation of undesirable phases if manufacturing processes are not carefully controlled. In particular, sigma phase (σ) is a Cr- and Mo-rich intermetallic phase, formed generally when DSS are by the temperature range from 600 to 900 °C, even for very short time periods. The precipitation of this phase induces detrimental effects in mechanical and corrosion resistance properties in the material, and even a low volume percentage of σ phase can significantly affect these properties. The current paper presents the effect of thermal treatments on UNS S32760 superduplex stainless steel seamless tubes, applied in order to promote the precipitation of different σ phase percentages in a ferrite/austenite microstructure. The detection and quantification of the σ phase using non-destructive ultrasounds testing has been one of the most relevant events of this study that contributes to improving the correlation of the results obtained using destructive and non-destructive techniques for the quantification of undesirable phases in superduplex seamless tubes during the manufacturing process.

Signal processing for non-destructive testing of railway tracks

NASA Astrophysics Data System (ADS)

Heckel, Thomas; Casperson, Ralf; Rühe, Sven; Mook, Gerhard

2018-04-01

Increased speed, heavier loads, altered material and modern drive systems result in an increasing number of rail flaws. The appearance of these flaws also changes continually due to the rapid change in damage mechanisms of modern rolling stock. Hence, interpretation has become difficult when evaluating non-destructive rail testing results. Due to the changed interplay between detection methods and flaws, the recorded signals may result in unclassified types of rail flaws. Methods for automatic rail inspection (according to defect detection and classification) undergo continual development. Signal processing is a key technology to master the challenge of classification and maintain resolution and detection quality, independent of operation speed. The basic ideas of signal processing, based on the Glassy-Rail-Diagram for classification purposes, are presented herein. Examples for the detection of damages caused by rolling contact fatigue also are given, and synergetic effects of combined evaluation of diverse inspection methods are shown.

Accuracy of Non-Destructive Testing of PBRs to Estimate Fragilities

NASA Astrophysics Data System (ADS)

Brune, J. N.; Brune, R.; Biasi, G. P.; Anooshehpoor, R.; Purvance, M.

2011-12-01

Prior studies of Precariously Balanced Rocks (PBRs) have involved various methods of documenting rock shapes and fragilities. These have included non-destructive testing (NDT) methods such as photomodeling, and potentially destructive testing (PDT) such as forced tilt tests. PDT methods usually have the potential of damaging or disturbing the rock or its pedestal so that the PBR usefulness for future generations is compromised. To date we have force-tilt tested approximately 28 PBRs, and of these we believe 7 have been compromised. We suggest here that given other inherent uncertainties in the current methodologies, NDT methods are now sufficiently advanced as to be adequate for the current state of the art use for comparison with Ground Motion Prediction Equations (GMPEs) and seismic hazard maps (SHMs). Here we compare tilt-test static toppling estimates to three non-destructive methods: (1) 3-D photographic modeling (2) profile analysis assuming the rock is 2-D, and (3) expert judgments from photographs. 3-D modeling uses the commercial Photomodeler program and photographs in the field taken from numerous directions around the rock. The output polyhedral shape is analyzed in Matlab determine the center of mass and in Autocad to estimate the static overturning angle alpha. For the 2-D method we chose the photograph in profile looking perpendicular to the estimated direction of toppling. The rock is outlined as a 2-D object in Matlab. Rock dimensions, rocking points, and a vertical reference are supplied by the photo analyst to estimate the center of gravity and static force overturning angles. For the expert opinion method we used additional photographs taken from different directions to improve the estimates of the center of mass and the rocking points. We used 7 rocks for comparisons. The error in estimating tan alpha from 3-D modeling is about 0.05. For 2-D estimates an average error is about 0.1 (?). For expert opinion estimates the error is about 0.06. For

Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review.

PubMed

Kim, Kang; Wagner, William R

2016-03-01

With the rapid expansion of biomaterial development and coupled efforts to translate such advances toward the clinic, non-invasive and non-destructive imaging tools to evaluate implants in situ in a timely manner are critically needed. The required multi-level information is comprehensive, including structural, mechanical, and biological changes such as scaffold degradation, mechanical strength, cell infiltration, extracellular matrix formation and vascularization to name a few. With its inherent advantages of non-invasiveness and non-destructiveness, ultrasound imaging can be an ideal tool for both preclinical and clinical uses. In this review, currently available ultrasound imaging technologies that have been applied in vitro and in vivo for tissue engineering and regenerative medicine are discussed and some new emerging ultrasound technologies and multi-modality approaches utilizing ultrasound are introduced.

Non-invasive and non-destructive characterization of tissue engineered constructs using ultrasound imaging technologies: a review

PubMed Central

Kim, Kang; Wagner, William R.

2015-01-01

With the rapid expansion of biomaterial development and coupled efforts to translate such advances toward the clinic, non-invasive and non-destructive imaging tools to evaluate implants in situ in a timely manner are critically needed. The required multilevel information is comprehensive, including structural, mechanical, and biological changes such as scaffold degradation, mechanical strength, cell infiltration, extracellular matrix formation and vascularization to name a few. With its inherent advantages of non-invasiveness and non-destructiveness, ultrasound imaging can be an ideal tool for both preclinical and clinical uses. In this review, currently available ultrasound imaging technologies that have been applied in vitro and in vivo for tissue engineering and regenerative medicine are discussed and some new emerging ultrasound technologies and multi-modality approaches utilizing ultrasound are introduced. PMID:26518412

Non-destructive mapping of grain orientations in 3D by laboratory X-ray microscopy

PubMed Central

McDonald, S. A.; Reischig, P.; Holzner, C.; Lauridsen, E. M.; Withers, P. J.; Merkle, A. P.; Feser, M.

2015-01-01

The ability to characterise crystallographic microstructure, non-destructively and in three-dimensions, is a powerful tool for understanding many aspects related to damage and deformation mechanisms in polycrystalline materials. To this end, the technique of X-ray diffraction contrast tomography (DCT) using monochromatic synchrotron and polychromatic laboratory X-ray sources has been shown to be capable of mapping crystal grains and their orientations non-destructively in 3D. Here we describe a novel laboratory-based X-ray DCT modality (LabDCT), enabling the wider accessibility of the DCT technique for routine use and in-depth studies of, for example, temporal changes in crystallographic grain structure non-destructively over time through ‘4D’ in situ time-lapse studies. The capability of the technique is demonstrated by studying a titanium alloy (Ti-β21S) sample. In the current implementation the smallest grains that can be reliably detected are around 40 μm. The individual grain locations and orientations are reconstructed using the LabDCT method and the results are validated against independent measurements from phase contrast tomography and electron backscatter diffraction respectively. Application of the technique promises to provide important insights related to the roles of recrystallization and grain growth on materials properties as well as supporting 3D polycrystalline modelling of materials performance. PMID:26494523

Spatial distribution pattern analysis of subtidal macroalgae assemblages by a non-destructive rapid assessment method

NASA Astrophysics Data System (ADS)

Guinda, Xabier; Juanes, José Antonio; Puente, Araceli; Echavarri-Erasun, Beatriz

2012-01-01

The extensive field work carried out over the last century has allowed the worldwide description of general distribution patterns and specific composition of rocky intertidal communities. However, the information concerning subtidal communities on hard substrates is more recent and scarce due to the difficulties associated with working in such environments. In this work, a non-destructive method is applied to the study and mapping of subtidal rocky bottom macroalgae assemblages on the coast of Cantabria (N Spain) which is quick, easy and economical. Gelidium corneum and Cystoseira baccata were the dominant species, however, the composition and coverage of macroalgae assemblages varied significantly at different locations and depth ranges. The high presence of Laminaria ochroleuca and Saccorhiza polyschides, characteristic of colder waters, shows the transitional character of this coastal area. The results obtained throughout this study have been very useful to the application of the European Water Framework Directive (WFD 2000/60/EC) and could be of great interest for the future conservation and management of these ecosystems (e.g. Habitats Directive 92/43/EEC).

Post-Irradiation Non-Destructive Analyses of the AFIP-7 Experiment

NASA Astrophysics Data System (ADS)

Williams, W. J.; Robinson, A. B.; Rabin, B. H.

2017-12-01

This article reports the results and interpretation of post-irradiation non-destructive examinations performed on four curved full-size fuel plates that comprise the AFIP-7 experiment. These fuel plates, having a U-10 wt.%Mo monolithic design, were irradiated under moderate operating conditions in the Advanced Test Reactor to assess fuel performance for geometries that are prototypic of research reactor fuel assemblies. Non-destructive examinations include visual examination, neutron radiography, profilometry, and precision gamma scanning. This article evaluates the qualitative and quantitative data taken for each plate, compares corresponding data sets, and presents the results of swelling analyses. These characterization results demonstrate that the fuel meets established irradiation performance requirements for mechanical integrity, geometric stability, and stable and predictable behavior.

Non-destructive determination of floral staging in cereals using X-ray micro computed tomography (µCT).

PubMed

Tracy, Saoirse R; Gómez, José Fernández; Sturrock, Craig J; Wilson, Zoe A; Ferguson, Alison C

2017-01-01

Accurate floral staging is required to aid research into pollen and flower development, in particular male development. Pollen development is highly sensitive to stress and is critical for crop yields. Research into male development under environmental change is important to help target increased yields. This is hindered in monocots as the flower develops internally in the pseudostem. Floral staging studies therefore typically rely on destructive analysis, such as removal from the plant, fixation, staining and sectioning. This time-consuming analysis therefore prevents follow up studies and analysis past the point of the floral staging. This study focuses on using X-ray µCT scanning to allow quick and detailed non-destructive internal 3D phenotypic information to allow accurate staging of Arabidopsis thaliana L. and Barley ( Hordeum vulgare L.) flowers. X-ray µCT has previously relied on fixation methods for above ground tissue, therefore two contrast agents (Lugol's iodine and Bismuth) were observed in Arabidopsis and Barley in planta to circumvent this step. 3D models and 2D slices were generated from the X-ray µCT images providing insightful information normally only available through destructive time-consuming processes such as sectioning and microscopy. Barley growth and development was also monitored over three weeks by X-ray µCT to observe flower development in situ. By measuring spike size in the developing tillers accurate non-destructive staging at the flower and anther stages could be performed; this staging was confirmed using traditional destructive microscopic analysis. The use of X-ray micro computed tomography (µCT) scanning of living plant tissue offers immense benefits for plant phenotyping, for successive developmental measurements and for accurate developmental timing for scientific measurements. Nevertheless, X-ray µCT remains underused in plant sciences, especially in above-ground organs, despite its unique potential in delivering

A Non-Destructive Method for Distinguishing Reindeer Antler (Rangifer tarandus) from Red Deer Antler (Cervus elaphus) Using X-Ray Micro-Tomography Coupled with SVM Classifiers

PubMed Central

Lefebvre, Alexandre; Rochefort, Gael Y.; Santos, Frédéric; Le Denmat, Dominique; Salmon, Benjamin; Pétillon, Jean-Marc

2016-01-01

Over the last decade, biomedical 3D-imaging tools have gained widespread use in the analysis of prehistoric bone artefacts. While initial attempts to characterise the major categories used in osseous industry (i.e. bone, antler, and dentine/ivory) have been successful, the taxonomic determination of prehistoric artefacts remains to be investigated. The distinction between reindeer and red deer antler can be challenging, particularly in cases of anthropic and/or taphonomic modifications. In addition to the range of destructive physicochemical identification methods available (mass spectrometry, isotopic ratio, and DNA analysis), X-ray micro-tomography (micro-CT) provides convincing non-destructive 3D images and analyses. This paper presents the experimental protocol (sample scans, image processing, and statistical analysis) we have developed in order to identify modern and archaeological antler collections (from Isturitz, France). This original method is based on bone microstructure analysis combined with advanced statistical support vector machine (SVM) classifiers. A combination of six microarchitecture biomarkers (bone volume fraction, trabecular number, trabecular separation, trabecular thickness, trabecular bone pattern factor, and structure model index) were screened using micro-CT in order to characterise internal alveolar structure. Overall, reindeer alveoli presented a tighter mesh than red deer alveoli, and statistical analysis allowed us to distinguish archaeological antler by species with an accuracy of 96%, regardless of anatomical location on the antler. In conclusion, micro-CT combined with SVM classifiers proves to be a promising additional non-destructive method for antler identification, suitable for archaeological artefacts whose degree of human modification and cultural heritage or scientific value has previously made it impossible (tools, ornaments, etc.). PMID:26901355

Non-destructive geographical traceability of sea cucumber (Apostichopus japonicus) using near infrared spectroscopy combined with chemometric methods

PubMed Central

Cai, Rui; Wang, Shisheng; Tang, Bo; Li, Yueqing; Zhao, Weijie

2018-01-01

Sea cucumber is the major tonic seafood worldwide, and geographical origin traceability is an important part of its quality and safety control. In this work, a non-destructive method for origin traceability of sea cucumber (Apostichopus japonicus) from northern China Sea and East China Sea using near infrared spectroscopy (NIRS) and multivariate analysis methods was proposed. Total fat contents of 189 fresh sea cucumber samples were determined and partial least-squares (PLS) regression was used to establish the quantitative NIRS model. The ordered predictor selection algorithm was performed to select feasible wavelength regions for the construction of PLS and identification models. The identification model was developed by principal component analysis combined with Mahalanobis distance and scaling to the first range algorithms. In the test set of the optimum PLS models, the root mean square error of prediction was 0.45, and correlation coefficient was 0.90. The correct classification rates of 100% were obtained in both identification calibration model and test model. The overall results indicated that NIRS method combined with chemometric analysis was a suitable tool for origin traceability and identification of fresh sea cucumber samples from nine origins in China. PMID:29410795

Non-destructive geographical traceability of sea cucumber (Apostichopus japonicus) using near infrared spectroscopy combined with chemometric methods.

PubMed

Guo, Xiuhan; Cai, Rui; Wang, Shisheng; Tang, Bo; Li, Yueqing; Zhao, Weijie

2018-01-01

Sea cucumber is the major tonic seafood worldwide, and geographical origin traceability is an important part of its quality and safety control. In this work, a non-destructive method for origin traceability of sea cucumber ( Apostichopus japonicus ) from northern China Sea and East China Sea using near infrared spectroscopy (NIRS) and multivariate analysis methods was proposed. Total fat contents of 189 fresh sea cucumber samples were determined and partial least-squares (PLS) regression was used to establish the quantitative NIRS model. The ordered predictor selection algorithm was performed to select feasible wavelength regions for the construction of PLS and identification models. The identification model was developed by principal component analysis combined with Mahalanobis distance and scaling to the first range algorithms. In the test set of the optimum PLS models, the root mean square error of prediction was 0.45, and correlation coefficient was 0.90. The correct classification rates of 100% were obtained in both identification calibration model and test model. The overall results indicated that NIRS method combined with chemometric analysis was a suitable tool for origin traceability and identification of fresh sea cucumber samples from nine origins in China.

Manipulation of Molecular Quantum Wavepackets with Ultrashort Laser Pulses for Non-destructive Detection of Volatile Explosives

DTIC Science & Technology

2013-02-01

DTRA-TR-12-65 Manipulation of Molecular Quantum Wavepackets with Ultrashort Laser Pulses for Non-destructive Detection of Volatile Explosives ...Manipulation of Molecular Quantum Wavepackets with Ultrashort Laser Pulses for Non-destructive Detection of Volatile Explosives HDTRA1-09-1-0021 Valery...destructive detection of volatile explosives . Moshe Shapiro1, Valery Milner1 and Jun Ye2 1University of British Columbia, Vancouver, Canada 2JILA

Non-destructive characterization of corroded glass surfaces by spectroscopic ellipsometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaspar, Tiffany C.; Reiser, Joelle T.; Ryan, Joseph V.

Characterization of the alteration layers that form on glass surfaces during corrosion processes provides valuable information on both the mechanisms and rate of glass alteration. In recent years, state-of-the-art materials and surface characterization techniques have been employed to study various aspects of the alteration layers that result from corrosion. In most cases, these techniques are destructive and thus can only be employed at the end of the corrosion experiment. We show that the alteration layers can be investigated by non-destructive spectroscopic ellipsometry (SE), which provides pertinent information on alteration layer thickness, morphology, and, through correlation of the index of refraction,more » porosity. SE measurements of silicate glass coupons altered in aqueous solutions of pH 3, 5, 7, 9, and 11 at 90 °C for 7 days are compared to cross-sectional secondary electron microscopy images. In most cases, quantitative agreement of the alteration layer thickness is obtained. The fractional porosity calculated from the index of refraction is lower than the porosity calculated from elemental analysis of the aqueous solutions, indicating that the alteration layer has compacted during corrosion or the subsequent supercritical CO 2 drying process. Our results confirm the utility of performing non-destructive SE measurements on corroded glass surfaces.« less

Non-destructive characterization of corroded glass surfaces by spectroscopic ellipsometry

DOE PAGES

Kaspar, Tiffany C.; Reiser, Joelle T.; Ryan, Joseph V.; ...

2017-11-03

Characterization of the alteration layers that form on glass surfaces during corrosion processes provides valuable information on both the mechanisms and rate of glass alteration. In recent years, state-of-the-art materials and surface characterization techniques have been employed to study various aspects of the alteration layers that result from corrosion. In most cases, these techniques are destructive and thus can only be employed at the end of the corrosion experiment. We show that the alteration layers can be investigated by non-destructive spectroscopic ellipsometry (SE), which provides pertinent information on alteration layer thickness, morphology, and, through correlation of the index of refraction,more » porosity. SE measurements of silicate glass coupons altered in aqueous solutions of pH 3, 5, 7, 9, and 11 at 90 °C for 7 days are compared to cross-sectional secondary electron microscopy images. In most cases, quantitative agreement of the alteration layer thickness is obtained. The fractional porosity calculated from the index of refraction is lower than the porosity calculated from elemental analysis of the aqueous solutions, indicating that the alteration layer has compacted during corrosion or the subsequent supercritical CO 2 drying process. Our results confirm the utility of performing non-destructive SE measurements on corroded glass surfaces.« less

A non-destructive method for quantifying small-diameter woody biomass in southern pine forests

Treesearch

D. Andrew Scott; Rick Stagg; Morris Smith

2006-01-01

Quantifying the impact of silvicultural treatments on woody understory vegetation largely has been accomplished by destructive sampling or through estimates of frequency and coverage. In studies where repeated measures of understory biomass across large areas are needed, destructive sampling and percent cover estimates are not satisfactory. For example, estimates of...

Non-destructive study of iron gall inks in manuscripts

NASA Astrophysics Data System (ADS)

Duh, Jelena; Krstić, Dragica; Desnica, Vladan; Fazinić, Stjepko

2018-02-01

The aim of this research is to establish an effective procedure of iron gall ink characterization using complementary non-destructive methods. By this, it is possible to better understand correlation of chemical composition of the inks and the state of preservation of iron gall ink manuscripts, as well as the effects of conservation treatment performed upon them. This study was undertaken on a bound 16th century manuscript comprised of different types of paper and ink from the National and University Library in Zagreb. Analytical methods used included Particle Induced X-ray Emission (PIXE) and X-ray Fluorescence (XRF). Paper fibers were identified by optical microscopy and the degradation state, as well as ink differentiation, transit metal migrations and detection of stains, with ultraviolet (UV) and infrared (IR) photography. The techniques applied on original writing materials gave important information about paper and ink composition, its preservation state and efficiency of conservation treatment performed upon them.

Shake and stew: a non-destructive PCR-ready DNA isolation method from a single preserved fish larva.

PubMed

Alvarado Bremer, J R; Smith, B L; Moulton, D L; Lu, C-P; Cornic, M

2014-01-01

A rapid non-destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor. © 2014 The Fisheries Society of the British Isles.

Non-destructive Determination of Shikimic Acid Concentration in Transgenic Maize Exhibiting Glyphosate Tolerance Using Chlorophyll Fluorescence and Hyperspectral Imaging

PubMed Central

Feng, Xuping; Yu, Chenliang; Chen, Yue; Peng, Jiyun; Ye, Lanhan; Shen, Tingting; Wen, Haiyong; He, Yong

2018-01-01

The development of transgenic glyphosate-tolerant crops has revolutionized weed control in crops in many regions of the world. The early, non-destructive identification of superior plant phenotypes is an important stage in plant breeding programs. Here, glyphosate-tolerant transgenic maize and its parental wild-type control were studied at 2, 4, 6, and 8 days after glyphosate treatment. Visible and near-infrared hyperspectral imaging and chlorophyll fluorescence imaging techniques were applied to monitor the performance of plants. In our research, transgenic maize, which was highly tolerant to glyphosate, was phenotyped using these high-throughput non-destructive methods to validate low levels of shikimic acid accumulation and high photochemical efficiency of photosystem II as reflected by maximum quantum yield and non-photochemical quenching in response to glyphosate. For hyperspectral imaging analysis, the combination of spectroscopy and chemometric methods was used to predict shikimic acid concentration. Our results indicated that a partial least-squares regression model, built on optimal wavelengths, effectively predicted shikimic acid concentrations, with a coefficient of determination value of 0.79 for the calibration set, and 0.82 for the prediction set. Moreover, shikimic acid concentration estimates from hyperspectral images were visualized on the prediction maps by spectral features, which could help in developing a simple multispectral imaging instrument for non-destructive phenotyping. Specific physiological effects of glyphosate affected the photochemical processes of maize, which induced substantial changes in chlorophyll fluorescence characteristics. A new data-driven method, combining mean fluorescence parameters and featuring a screening approach, provided a satisfactory relationship between fluorescence parameters and shikimic acid content. The glyphosate-tolerant transgenic plants can be identified with the developed discrimination model

Non-destructive Determination of Shikimic Acid Concentration in Transgenic Maize Exhibiting Glyphosate Tolerance Using Chlorophyll Fluorescence and Hyperspectral Imaging.

PubMed

Feng, Xuping; Yu, Chenliang; Chen, Yue; Peng, Jiyun; Ye, Lanhan; Shen, Tingting; Wen, Haiyong; He, Yong

2018-01-01

The development of transgenic glyphosate-tolerant crops has revolutionized weed control in crops in many regions of the world. The early, non-destructive identification of superior plant phenotypes is an important stage in plant breeding programs. Here, glyphosate-tolerant transgenic maize and its parental wild-type control were studied at 2, 4, 6, and 8 days after glyphosate treatment. Visible and near-infrared hyperspectral imaging and chlorophyll fluorescence imaging techniques were applied to monitor the performance of plants. In our research, transgenic maize, which was highly tolerant to glyphosate, was phenotyped using these high-throughput non-destructive methods to validate low levels of shikimic acid accumulation and high photochemical efficiency of photosystem II as reflected by maximum quantum yield and non-photochemical quenching in response to glyphosate. For hyperspectral imaging analysis, the combination of spectroscopy and chemometric methods was used to predict shikimic acid concentration. Our results indicated that a partial least-squares regression model, built on optimal wavelengths, effectively predicted shikimic acid concentrations, with a coefficient of determination value of 0.79 for the calibration set, and 0.82 for the prediction set. Moreover, shikimic acid concentration estimates from hyperspectral images were visualized on the prediction maps by spectral features, which could help in developing a simple multispectral imaging instrument for non-destructive phenotyping. Specific physiological effects of glyphosate affected the photochemical processes of maize, which induced substantial changes in chlorophyll fluorescence characteristics. A new data-driven method, combining mean fluorescence parameters and featuring a screening approach, provided a satisfactory relationship between fluorescence parameters and shikimic acid content. The glyphosate-tolerant transgenic plants can be identified with the developed discrimination model

Non-destructive NIR-FT-Raman analyses in practice. Part I. Analyses of plants and historic textiles.

PubMed

Andreev, G N; Schrader, B; Schulz, H; Fuchs, R; Popov, S; Handjieva, N

2001-12-01

Non-destructive analysis of natural substances in plants as well as of old dyed textiles by Raman spectroscopy has not been possible using conventional techniques. Exciting lines from the visible part of the spectrum produced photochemical and thermal decomposition of the objects as well as strong fluorescence. Using Nd:YAG laser excitation at 1,064 nm together with a special sample arrangement and interferometric recording, various polyacetylenes in Aethusa cynapium and in chamomile (Chamomilla recutita) and the main valuable substances in gentian species (Gentiana lutea and G. punctata), curcuma roots (Curcuma longa), cinnamon (Cinnamomum zeylanicum), fennel (Foeniculum vulgare), clove (Caryophyllus aromaticus), and ginger (Zingiber officinale) were analyzed non-destructively and discussed in comparison with the corresponding pure standard compounds. We further analyzed non-destructively the FT Raman spectra of collections of historical textiles and lakes used for dyeing. It is possible to distinguish the main dye component non-destructively by using Raman bands.

Non-Destructive Evaluation of Grain Structure Using Air-Coupled Ultrasonics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Belvin, A. D.; Burrell, R. K.; Cole, E.G.

2009-08-01

Cast material has a grain structure that is relatively non-uniform. There is a desire to evaluate the grain structure of this material non-destructively. Traditionally, grain size measurement is a destructive process involving the sectioning and metallographic imaging of the material. Generally, this is performed on a representative sample on a periodic basis. Sampling is inefficient and costly. Furthermore, the resulting data may not provide an accurate description of the entire part's average grain size or grain size variation. This project is designed to develop a non-destructive acoustic scanning technique, using Chirp waveforms, to quantify average grain size and grain sizemore » variation across the surface of a cast material. A Chirp is a signal in which the frequency increases or decreases over time (frequency modulation). As a Chirp passes through a material, the material's grains reduce the signal (attenuation) by absorbing the signal energy. Geophysics research has shown a direct correlation with Chirp wave attenuation and mean grain size in geological structures. The goal of this project is to demonstrate that Chirp waveform attenuation can be used to measure grain size and grain variation in cast metals (uranium and other materials of interest). An off-axis ultrasonic inspection technique using air-coupled ultrasonics has been developed to determine grain size in cast materials. The technique gives a uniform response across the volume of the component. This technique has been demonstrated to provide generalized trends of grain variation over the samples investigated.« less

Computed tomography for non-destructive evaluation of composites: Applications and correlations

NASA Technical Reports Server (NTRS)

Goldberg, B.; Hediger, L.; Noel, E.

1985-01-01

The state-of-the-art fabrication techniques for composite materials are such that stringent species-specific acceptance criteria must be generated to insure product reliability. Non-destructive evaluation techniques including computed tomography (CT), X-ray radiography (RT), and ultrasonic scanning (UT) are investigated and compared to determine their applicability and limitations to graphite epoxy, carbon-carbon, and carbon-phenolic materials. While the techniques appear complementary, CT is shown to provide significant, heretofore unattainable data. Finally, a correlation of NDE techniques to destructive analysis is presented.

Non-destructive characterization of SiC coated carbon-carbon composites by multiple techniques

NASA Astrophysics Data System (ADS)

Nixon, Thomas D.; Hemstad, Stan N.; Pfeifer, William H.

SiC coated carbon-carbon composites were evaluated using several non-destructive techniques as a means of quantifying the quality of both the coating and substrate. The techniques employed included dye penetrant infiltration, eddy current measurement, C-scan, and computed tomography (CT). The NDE results were then correlated to oxidation performance and destructive evaluations by electron and optical microscopy.

Non-destructive controlled single-particle light scattering measurement

NASA Astrophysics Data System (ADS)

Maconi, G.; Penttilä, A.; Kassamakov, I.; Gritsevich, M.; Helander, P.; Puranen, T.; Salmi, A.; Hæggström, E.; Muinonen, K.

2018-01-01

We present a set of light scattering data measured from a millimeter-sized extraterrestrial rock sample. The data were acquired by our novel scatterometer, which enables accurate multi-wavelength measurements of single-particle samples whose position and orientation are controlled by ultrasonic levitation. The measurements demonstrate a non-destructive approach to derive optical properties of small mineral samples. This enables research on valuable materials, such as those returned from space missions or rare meteorites.

Advances in Non-Destructive Early Assessment of Fruit Ripeness towards Defining Optimal Time of Harvest and Yield Prediction—A Review

PubMed Central

Lecourt, Julien; Bishop, Gerard

2018-01-01

Global food security for the increasing world population not only requires increased sustainable production of food but a significant reduction in pre- and post-harvest waste. The timing of when a fruit is harvested is critical for reducing waste along the supply chain and increasing fruit quality for consumers. The early in-field assessment of fruit ripeness and prediction of the harvest date and yield by non-destructive technologies have the potential to revolutionize farming practices and enable the consumer to eat the tastiest and freshest fruit possible. A variety of non-destructive techniques have been applied to estimate the ripeness or maturity but not all of them are applicable for in situ (field or glasshouse) assessment. This review focuses on the non-destructive methods which are promising for, or have already been applied to, the pre-harvest in-field measurements including colorimetry, visible imaging, spectroscopy and spectroscopic imaging. Machine learning and regression models used in assessing ripeness are also discussed. PMID:29320410

Non-destructive measurement of soil liquefaction density change by crosshole radar tomography, Treasure Island, California

USGS Publications Warehouse

Kayen, Robert E.; Barnhardt, Walter A.; Ashford, Scott; Rollins, Kyle

2000-01-01

A ground penetrating radar (GPR) experiment at the Treasure Island Test Site [TILT] was performed to non-destructively image the soil column for changes in density prior to, and following, a liquefaction event. The intervening liquefaction was achieved by controlled blasting. A geotechnical borehole radar technique was used to acquire high-resolution 2-D radar velocity data. This method of non-destructive site characterization uses radar trans-illumination surveys through the soil column and tomographic data manipulation techniques to construct radar velocity tomograms, from which averaged void ratios can be derived at 0.25 - 0.5m pixel footprints. Tomograms of void ratio were constructed through the relation between soil porosity and dielectric constant. Both pre- and post-blast tomograms were collected and indicate that liquefaction related densification occurred at the site. Volumetric strains estimated from the tomograms correlate well with the observed settlement at the site. The 2-D imagery of void ratio can serve as high-resolution data layers for numerical site response analysis.

Identification of the Thickness of Nugget on Worksheet Spot Welding Using Non Destructive Test (NDT) - Effect of Pressure

NASA Astrophysics Data System (ADS)

Sifa, A.; Baskoro, A. S.; Sugeng, S.; Badruzzaman, B.; Endramawan, T.

2018-02-01

Resistance Spot Welding (RSW) is a process of connecting between two worksheet with thermomechanical loading process, RSW is widely used in automotive industry, the quality of splicing spot welding is influenced by several factors. One of the factors at the time of the welding process is pressure. The quality of welding on the nuggets can be determined by undertaking non-destructive testing by using Non Destructive Test (NDT) - Ultrasonic Test. In the NDT test is done by detecting the thickness of the nugget area, the purpose of research conducted to determine the effect of pressure to welding quality with Nugget thickness gauge measurement with Non Destructive Test method and manual measurement with micrometer, Experimental welding process by entering the welding parameters that have been specified and pressure variables 1 -5 bars on the worksheet thickness of 1 mm. The results of testing with NDT show there is addition of thickness in nugget superiority after compare with measurement result of thickness of nugget with micrometer which slightly experience thickness in nugget area, this indicates that the welding results have a connection between worksheet 1 and worksheet 2.

Electromagnetic non-destructive technique for duplex stainless steel characterization

NASA Astrophysics Data System (ADS)

Rocha, João Vicente; Camerini, Cesar; Pereira, Gabriela

2016-02-01

Duplex stainless steel (DSS) is a two-phase (ferrite and austenite) material, which exhibits an attractive combination of mechanical properties and high corrosion resistance, being commonly employed for equipment of petrochemical plants, refining units and oil & gas platforms. The best properties of DSS are achieved when the phases are in equal proportions. However, exposition to high temperatures (e.g. welding process) may entail undesired consequences, such as deleterious phases precipitation (e.g. sigma, chi) and different proportion of the original phases, impairing dramatically the mechanical and corrosion properties of the material. A detailed study of the magnetic behavior of DSS microstructure with different ferrite austenite ratios and deleterious phases content was accomplished. The non destructive method evaluates the electromagnetic properties changes in the material and is capable to identify the presence of deleterious phases into DSS microstructure.

Template synthesis of test tube nanoparticles using non-destructive replication

PubMed Central

Wagner, Jonathan; Yao, Jingyuan; Rodgers, David; Hinds, Bruce

2013-01-01

Nano test tubes are a promising delivery vehicle for a range of therapeutics including small molecule drugs and biologics. However, current template synthesis methods of producing nano test tubes are prohibitively expensive and time consuming. Here, non-destructive template replication was used to increase nano test tube yield from porous alumina by more than a hundredfold. We demonstrate how to produce nano test tubes of several sizes and compositions including hybrid tubes with different inner and outer surfaces for targeted surface chemistry. Nano test tubes were readily suspended and stored in aqueous solutions without the need for chemical treatment. These nano test tubes should find application as delivery vehicles for therapeutics, particularly for processive “bionanoreactors” loaded with enzymes. PMID:23376956

EURATOM safeguards efforts in the development of spent fuel verification methods by non-destructive assay

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matloch, L.; Vaccaro, S.; Couland, M.

The back end of the nuclear fuel cycle continues to develop. The European Commission, particularly the Nuclear Safeguards Directorate of the Directorate General for Energy, implements Euratom safeguards and needs to adapt to this situation. The verification methods for spent nuclear fuel, which EURATOM inspectors can use, require continuous improvement. Whereas the Euratom on-site laboratories provide accurate verification results for fuel undergoing reprocessing, the situation is different for spent fuel which is destined for final storage. In particular, new needs arise from the increasing number of cask loadings for interim dry storage and the advanced plans for the construction ofmore » encapsulation plants and geological repositories. Various scenarios present verification challenges. In this context, EURATOM Safeguards, often in cooperation with other stakeholders, is committed to further improvement of NDA methods for spent fuel verification. In this effort EURATOM plays various roles, ranging from definition of inspection needs to direct participation in development of measurement systems, including support of research in the framework of international agreements and via the EC Support Program to the IAEA. This paper presents recent progress in selected NDA methods. These methods have been conceived to satisfy different spent fuel verification needs, ranging from attribute testing to pin-level partial defect verification. (authors)« less

Non-destructive testing for the structures and civil infrastructures characterization

NASA Astrophysics Data System (ADS)

Capozzoli, L.; Rizzo, E.

2012-04-01

infrared thermography and sonic testing. Finally, we investigated a radiant floor by GPR (900 MHz to 2000 MHz antennas) and long-wave infrared camera. Non-destructive diagnostic techniques allow to investigate a building structure in reinforced concrete or masonry without altering the characteristics of the element investigated. For this reason, geo-electrical and electromagnetic surveys of masonry are a suitable non-destructive tool for the diagnosis of a deteriorated concrete structure. Moreover, the integration of different NDT techniques (conventional and no-conventional) is a very powerful to maximize the capabilities and to compensate for the limitations of each method.

Testing an Impedance Non-destructive Method to Evaluate Steel-Fiber Concrete Samples

NASA Astrophysics Data System (ADS)

Komarkova, Tereza; Fiala, Pavel; Steinbauer, Miloslav; Roubal, Zdenek

2018-02-01

Steel-fiber reinforced concrete is a composite material characterized by outstanding tensile properties and resistance to the development of cracks. The concrete, however, exhibits such characteristics only on the condition that the steel fibers in the final, hardened composite have been distributed evenly. The current methods to evaluate the distribution and concentration of a fiber composite are either destructive or exhibit a limited capability of evaluating the concentration and orientation of the fibers. In this context, the paper discusses tests related to the evaluation of the density and orientation of fibers in a composite material. Compared to the approaches used to date, the proposed technique is based on the evaluation of the electrical impedance Z in the band close to the resonance of the sensor-sample configuration. Using analytically expressed equations, we can evaluate the monitored part of the composite and its density at various depths of the tested sample. The method employs test blocks of composites, utilizing the resonance of the measuring device and the measured sample set; the desired state occurs within the interval of between f=3 kHz and 400 kHz.

A Non-Destructive and Direction-Insensitive Method Using a Strain Sensor and Two Single Axis Angle Sensors for Evaluating Corn Stalk Lodging Resistance.

PubMed

Guo, Qingqian; Chen, Ruipeng; Sun, Xiaoquan; Jiang, Min; Sun, Haifeng; Wang, Shun; Ma, Liuzheng; Yang, Yatao; Hu, Jiandong

2018-06-06

Corn stalk lodging is caused by different factors, including severe wind storms, stalk cannibalization, and stalk rots, and it leads to yield loss. Determining how to rapidly evaluate corn lodging resistance will assist scientists in the field of crop breeding to understand the contributing factors in managing the moisture, chemical fertilizer, and weather conditions for corn growing. This study proposes a non-destructive and direction-insensitive method, using a strain sensor and two single axis angle sensors to measure the corn stalk lodging resistance in the field. An equivalent force whose direction is perpendicular to the stalk is utilized to evaluate the corn lodging properties when a pull force is applied on the corn stalk. A novel measurement device is designed to obtain the equivalent force with the coefficient of variation (CV) of 4.85%. Five corn varieties with two different planting densities are arranged to conduct the experiment using the novel measurement device. The experimental results show that the maximum equivalent force could reach up to 44 N. A strong relationship with the square of the correlation coefficient of 0.88 was obtained between the maximum equivalent forces and the corn field’s stalk lodging rates. Moreover, the stalk lodging angles corresponding to the different pull forces over a measurement time of 20 s shift monotonically along the equivalent forces. Thus, the non-destructive and direction-insensitive method is an excellent tool for rapid analysis of stalk lodging resistance in corn, providing critical information on in-situ lodging dynamics.

Complex Archaeological Prospection Using Combination of Non-destructive Techniques

NASA Astrophysics Data System (ADS)

Faltýnová, M.; Pavelka, K.; Nový, P.; Šedina, J.

2015-08-01

This article describes the use of a combination of non-destructive techniques for the complex documentation of a fabulous historical site called Devil's Furrow, an unusual linear formation lying in the landscape of central Bohemia. In spite of many efforts towards interpretation of the formation, its original form and purpose have not yet been explained in a satisfactory manner. The study focuses on the northern part of the furrow which appears to be a dissimilar element within the scope of the whole Devil's Furrow. This article presents detailed description of relics of the formation based on historical map searches and modern investigation methods including airborne laser scanning, aerial photogrammetry (based on airplane and RPAS) and ground-penetrating radar. Airborne laser scanning data and aerial orthoimages acquired by the Czech Office for Surveying, Mapping and Cadastre were used. Other measurements were conducted by our laboratory. Data acquired by various methods provide sufficient information to determine the probable original shape of the formation and proves explicitly the anthropological origin of the northern part of the formation (around village Lipany).

Application of finite elements heterogeneous multi-scale method to eddy currents non destructive testing of carbon composites material

NASA Astrophysics Data System (ADS)

Khebbab, Mohamed; Feliachi, Mouloud; El Hadi Latreche, Mohamed

2018-03-01

In this present paper, a simulation of eddy current non-destructive testing (EC NDT) on unidirectional carbon fiber reinforced polymer is performed; for this magneto-dynamic formulation in term of magnetic vector potential is solved using finite element heterogeneous multi-scale method (FE HMM). FE HMM has as goal to compute the homogenized solution without calculating the homogenized tensor explicitly, the solution is based only on the physical characteristic known in micro domain. This feature is well adapted to EC NDT to evaluate defect in carbon composite material in microscopic scale, where the defect detection is performed by coil impedance measurement; the measurement value is intimately linked to material characteristic in microscopic level. Based on this, our model can handle different defects such as: cracks, inclusion, internal electrical conductivity changes, heterogeneities, etc. The simulation results were compared with the solution obtained with homogenized material using mixture law, a good agreement was found.

The non-destructive identification of early Chinese porcelain by PIXE

NASA Astrophysics Data System (ADS)

Cheng, H. S.; Zhang, Z. Q.; Zhang, B.; Yang, F. J.

2004-06-01

PIXE is used for the non-destructive differentiation of early precious Chinese blue and white porcelain made in Yuan (AD 1206-1368), Ming (AD 1368-1644) Dynasty in Jingdezhen from imitations. Also, ancient celadon made in Song Dynasty (AD 960-1279) is identified by measuring the trace elements contained in the glazes.

NON-DESTRUCTIVE FLAW DETECTION APPARATUS

DOEpatents

Stateman, M.J.; Holloway, H.R.

1957-12-17

An apparatus is described for the non-destructive detection of flaws in electrical conducting articles. The particular feature of the detection apparatus is that a flaw in the front or back of the test article will not be masked by signals caused by the passage of the end and front of the article through the detection apparatus. The present invention alleviates the above problem by mounting detection coils on directly opposite sides of the test passageway so that the axes of the pickup coils are perpendicular to the axis of an energizing coil through which the article is passed. A flaw in the article will cause a change in the voltage induced in one pickup coil, but passage of the end or front of the article will not produce unequal signals. The signals are compared in appropriate electrical circuitry to actuate a recorder only when unequal signals are present, indicating the presence of a flaw.

Gender differentiation in indirect self-destructiveness and suicide attempt methods (gender, indirect self-destructiveness, and suicide attempts).

PubMed

Tsirigotis, Konstantinos; Gruszczyński, Wojciech; Tsirigotis-Maniecka, Marta

2014-06-01

The objective of this study is to examine the gender (sex) differentiation of indirect self-destructiveness and its manifestations as well as its relationships with suicide attempt methods in females and males. The study was conducted among 147 persons (114 females, 33 males) who attempted suicide. The research instrument was the polish version of the Chronic Self-Destructiveness Scale including Transgression and Risk, Poor Health Maintenance, Personal and Social Neglects, Lack of Planfulness, and Helplessness and Passiveness in the face of problems. Differences testing and correlation analyses were applied. Females scored higher on poor health maintenance and males scored significantly higher on personal and social neglects, lack of planfulness, and helplessness. Noteworthy is that the intensity of indirect self-destructiveness in females reached the same magnitude as in males. A number of statistically significant correlations were found between indirect self-destructiveness, or its manifestations, and the methods of suicide attempt in the two groups. Among these categories, the highest contribution was of helplessness and passiveness (both of groups), poor health maintenance (males), and personal and social neglects (females). Results of this study can be useful in the therapeutic efforts and prevention of not only indirectly self-destructive behaviours but also possible suicide attempts. Both preventive and therapeutic activities can take into account the specificity of those phenomena resulting from one's sex/gender. It is important to adapt preventive and therapeutic measures to psychological (personal) features that arise from an individual's sex/gender.

Rapid non-destructive assessment of pork edible quality by using VIS/NIR spectroscopic technique

NASA Astrophysics Data System (ADS)

Zhang, Leilei; Peng, Yankun; Dhakal, Sagar; Song, Yulin; Zhao, Juan; Zhao, Songwei

2013-05-01

The objectives of this research were to develop a rapid non-destructive method to evaluate the edible quality of chilled pork. A total of 42 samples were packed in seal plastic bags and stored at 4°C for 1 to 21 days. Reflectance spectra were collected from visible/near-infrared spectroscopy system in the range of 400nm to 1100nm. Microbiological, physicochemical and organoleptic characteristics such as the total viable counts (TVC), total volatile basic-nitrogen (TVB-N), pH value and color parameters L* were determined to appraise pork edible quality. Savitzky-Golay (SG) based on five and eleven smoothing points, Multiple Scattering Correlation (MSC) and first derivative pre-processing methods were employed to eliminate the spectra noise. The support vector machines (SVM) and partial least square regression (PLSR) were applied to establish prediction models using the de-noised spectra. A linear correlation was developed between the VIS/NIR spectroscopy and parameters such as TVC, TVB-N, pH and color parameter L* indexes, which could gain prediction results with Rv of 0.931, 0.844, 0.805 and 0.852, respectively. The results demonstrated that VIS/NIR spectroscopy technique combined with SVM possesses a powerful assessment capability. It can provide a potential tool for detecting pork edible quality rapidly and non-destructively.

Interdigital Capacitance Local Non-Destructive Examination of Nuclear Power Plant Cable for Aging Management Programs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glass, Samuel W.; Fifield, Leonard S.; Bowler, Nicola

This Pacific Northwest National Laboratory milestone report describes progress to date on the investigation of non-destructive test methods focusing on local cable insulation and jacket testing using an interdigital capacitance (IDC) approach. Earlier studies have assessed a number of non-destructive examination (NDE) methods for bulk, distributed, and local cable tests. A typical test strategy is to perform bulk assessments of the cable response using dielectric spectroscopy, Tan , or partial discharge followed by distributed tests like time domain reflectometry or frequency domain reflectometry to identify the most likely defect location followed by a local test that can include visual inspection,more » indenter modulus tests, or Fourier Transform Infrared Spectroscopy (FTIR) or Near Infrared Spectroscopy FTIR (FTNIR). If a cable is covered with an overlaying jacket, the jacket’s condition is likely to be more severely degraded than the underlying insulation. None of the above local test approaches can be used to evaluate insulation beneath a cable jacket. Since the jacket’s function is neither structural nor electrical, a degraded jacket may not have any significance regarding the cable’s performance or suitability for service. IDC measurements offer a promising alternative or complement to these local test approaches including the possibility to test insulation beneath an overlaying jacket.« less

Non-destructive system to evaluate critical properties of asphalt compaction : [research brief].

DOT National Transportation Integrated Search

2013-12-01

The Wisconsin Highway Research Program sponsored a two-stage investigation to develop a non-destructive system to evaluate critical compaction properties and characteristics of asphalt pavements during the densification process. Stage One activities ...

DNA recovery from microhymenoptera using six non-destructive methodologies with considerations for subsequent preparation of museum slides.

PubMed

Guzmán-Larralde, Adriana J; Suaste-Dzul, Alba P; Gallou, Adrien; Peña-Carrillo, Kenzy I

2017-01-01

Because of the tiny size of microhymenoptera, successful morphological identification typically requires specific mounting protocols that require time, skills, and experience. Molecular taxonomic identification is an alternative, but many DNA extraction protocols call for maceration of the whole specimen, which is not compatible with preserving museum vouchers. Thus, non-destructive DNA isolation methods are attractive alternatives for obtaining DNA without damaging sample individuals. However, their performance needs to be assessed in microhymenopterans. We evaluated six non-destructive methods: (A) DNeasy® Blood & Tissue Kit; (B) DNeasy® Blood & Tissue Kit, modified; (C) Protocol with CaCl 2 buffer; (D) Protocol with CaCl 2 buffer, modified; (E) HotSHOT; and (F) Direct PCR. The performance of each DNA extraction method was tested across several microhymenopteran species by attempting to amplify the mitochondrial gene COI from insect specimens of varying ages: 1 day, 4 months, 3 years, 12 years, and 23 years. Methods B and D allowed COI amplification in all insects, while methods A, C, and E were successful in DNA amplification from insects up to 12 years old. Method F, the fastest, was useful in insects up to 4 months old. Finally, we adapted permanent slide preparation in Canada balsam for every technique. The results reported allow for combining morphological and molecular methodologies for taxonomic studies.

Local defect resonance for sensitive non-destructive testing

NASA Astrophysics Data System (ADS)

Adebahr, W.; Solodov, I.; Rahammer, M.; Gulnizkij, N.; Kreutzbruck, M.

2016-02-01

Ultrasonic wave-defect interaction is a background of ultrasound activated techniques for imaging and non-destructive testing (NDT) of materials and industrial components. The interaction, primarily, results in acoustic response of a defect which provides attenuation and scattering of ultrasound used as an indicator of defects in conventional ultrasonic NDT. The derivative ultrasonic-induced effects include e.g. nonlinear, thermal, acousto-optic, etc. responses also applied for NDT and defect imaging. These secondary effects are normally relatively inefficient so that the corresponding NDT techniques require an elevated acoustic power and stand out from conventional ultrasonic NDT counterparts for their specific instrumentation particularly adapted to high-power ultrasonic. In this paper, a consistent way to enhance ultrasonic, optical and thermal defect responses and thus to reduce an ultrasonic power required is suggested by using selective ultrasonic activation of defects based on the concept of local defect resonance (LDR). A strong increase in vibration amplitude at LDR enables to reliably detect and visualize the defect as soon as the driving ultrasonic frequency is matched to the LDR frequency. This also provides a high frequency selectivity of the LDR-based imaging, i.e. an opportunity of detecting a certain defect among a multitude of other defects in material. Some examples are shown how to use LDR in non-destructive testing techniques, like vibrometry, ultrasonic thermography and shearography in order to enhance the sensitivity of defect visualization.

Response to: Dittrich et al.: Non-Embryo-Destructive Extraction of Pluripotent Embryonic Stem Cells – Overlooked Legal Prohibitions, Professional Legal Consequences and Inconsistencies in Patent Law

PubMed Central

Faltus, T.; Storz, U.

2016-01-01

The publication of “Non-embryo-destructive Extraction of Pluripotent Embryonic Stem Cells: Implications for Regenerative Medicine and Reproductive Medicine” by Dittrich et al. in Geburtshilfe und Frauenheilkunde 2015; 75: 1239–1242 1 describes various possibilities which could result from the non-embryo-destructive extraction of embryonic stem cells from human blastocysts. But implementing this method is more problematic, both legally and ethically, than the authors have represented it to be and is illegal in Germany. German patent DE 10 2004 062 184 on the non-embryo-destructive extraction of embryonic stem cells referred to by Dittrich et al. contravenes the higher-ranking case-law of the European Court of Justice. Ultimately, the non-embryo-destructive harvesting of embryonic stem cells with the aim of storing these cells for use in potential therapies as proposed by Dittrich et al. is prohibited in Germany and could lead to criminal prosecution. PMID:28094826

APPARATUS FOR NON-DESTRUCTIVE INSPECTION OF CANTILEVERED MEMBERS

DOEpatents

Taylor, E.R.; Mahoney, C.H.; Lay, C.R.

1961-10-24

An apparatus for non-destructive inspection of cantilevered members, such as compressor blades, is described. The member under inspection is vibrated with a regulated source of air under pressure. The amplitude of vibration of the member is maintained at its natural frequency. The frequency of vibration of the member is measured. An indication of an excessive decay or erratic shifting in the measured frequency above an allowable hysteretic decay is provided as an indication of a fault in the member. The member is vibrated for a selected test period. (AEC)

Wave field features of shallow vertical discontinuity and their application in non-destructive detection

USGS Publications Warehouse

Liu, J.; Xia, J.; Luo, Y.; Chen, C.; Li, X.; Huang, Y.

2007-01-01

The geotechnical integrity of critical infrastructure can be seriously compromised by the presence of fractures or crevices. Non-destructive techniques to accurately detect fractures in critical infrastructure such as dams and highways could be of significant benefit to the geotechnical industry. This paper investigates the application of shallow seismic and georadar methods to the detection of a vertical discontinuity using numerical simulations. The objective is to address the kinematical analysis of a vertical discontinuity, determine the resulting wave field characteristics, and provide the basis for determining the existence of vertical discontinuities based on the recorded signals. Simulation results demonstrate that: (1) A reflection from a vertical discontinuity produces a hyperbolic feature on a seismic or georadar profile; (2) In order for a reflection from a vertical discontinuity to be produced, a reflecting horizon below the discontinuity must exist, the offset between source and receiver (x0) must be non-zero, on the same side of the vertical discontinuity; (3) The range of distances from the vertical discontinuity where a reflection event is observed is proportional to its length and to x0; (4) Should the vertical crevice (or fracture) pass through a reflecting horizon, dual hyperbolic features can be observed on the records, and this can be used as a determining factor that the vertical crevice passes through the interface; and (5) diffractions from the edges of the discontinuity can be recorded with relatively smaller amplitude than reflections and their ranges are not constrained by the length of discontinuity. If the length of discontinuity is short enough, diffractions are the dominant feature. Real-world examples show that the shallow seismic reflection method and the georadar method are capable of recording the hyperbolic feature, which can be interpreted as vertical discontinuity. Thus, these methods show some promise as effective non-destructive

Local destruction of superconductivity by non-magnetic impurities in mesoscopic iron-based superconductors

NASA Astrophysics Data System (ADS)

Li, Jun; Ji, Min; Schwarz, Tobias; Ke, Xiaoxing; van Tendeloo, Gustaaf; Yuan, Jie; Pereira, Paulo J.; Huang, Ya; Zhang, Gufei; Feng, Hai-Luke; Yuan, Ya-Hua; Hatano, Takeshi; Kleiner, Reinhold; Koelle, Dieter; Chibotaru, Liviu F.; Yamaura, Kazunari; Wang, Hua-Bing; Wu, Pei-Heng; Takayama-Muromachi, Eiji; Vanacken, Johan; Moshchalkov, Victor V.

2015-07-01

The determination of the pairing symmetry is one of the most crucial issues for the iron-based superconductors, for which various scenarios are discussed controversially. Non-magnetic impurity substitution is one of the most promising approaches to address the issue, because the pair-breaking mechanism from the non-magnetic impurities should be different for various models. Previous substitution experiments demonstrated that the non-magnetic zinc can suppress the superconductivity of various iron-based superconductors. Here we demonstrate the local destruction of superconductivity by non-magnetic zinc impurities in Ba0.5K0.5Fe2As2 by exploring phase-slip phenomena in a mesoscopic structure with 119 × 102 nm2 cross-section. The impurities suppress superconductivity in a three-dimensional `Swiss cheese'-like pattern with in-plane and out-of-plane characteristic lengths slightly below ~1.34 nm. This causes the superconducting order parameter to vary along abundant narrow channels with effective cross-section of a few square nanometres. The local destruction of superconductivity can be related to Cooper pair breaking by non-magnetic impurities.

Local destruction of superconductivity by non-magnetic impurities in mesoscopic iron-based superconductors.

PubMed

Li, Jun; Ji, Min; Schwarz, Tobias; Ke, Xiaoxing; Van Tendeloo, Gustaaf; Yuan, Jie; Pereira, Paulo J; Huang, Ya; Zhang, Gufei; Feng, Hai-Luke; Yuan, Ya-Hua; Hatano, Takeshi; Kleiner, Reinhold; Koelle, Dieter; Chibotaru, Liviu F; Yamaura, Kazunari; Wang, Hua-Bing; Wu, Pei-Heng; Takayama-Muromachi, Eiji; Vanacken, Johan; Moshchalkov, Victor V

2015-07-03

The determination of the pairing symmetry is one of the most crucial issues for the iron-based superconductors, for which various scenarios are discussed controversially. Non-magnetic impurity substitution is one of the most promising approaches to address the issue, because the pair-breaking mechanism from the non-magnetic impurities should be different for various models. Previous substitution experiments demonstrated that the non-magnetic zinc can suppress the superconductivity of various iron-based superconductors. Here we demonstrate the local destruction of superconductivity by non-magnetic zinc impurities in Ba0.5K0.5Fe2As2 by exploring phase-slip phenomena in a mesoscopic structure with 119 × 102 nm(2) cross-section. The impurities suppress superconductivity in a three-dimensional 'Swiss cheese'-like pattern with in-plane and out-of-plane characteristic lengths slightly below ∼1.34 nm. This causes the superconducting order parameter to vary along abundant narrow channels with effective cross-section of a few square nanometres. The local destruction of superconductivity can be related to Cooper pair breaking by non-magnetic impurities.

A New Non-Destructive TDR System Combined with a Piezoelectric Stack for Measuring Properties of Geomaterials

PubMed Central

Choi, Chanyong; Song, Minwoo; Kim, Daehyeon; Yu, Xiong

2016-01-01

Dry density and water content are two important factors affecting the degree of soil compaction. Conventional methods such as the sand cone test and the plate load test are used to measure such properties for evaluating the degree of compaction and the stiffness of soil in the field. However, these tests are generally very time-consuming and are inherent with some errors depending on the operator (in particular for the sand cone test). Elastic modulus is an indicator to describe the stress-strain behavior of soil and in some cases is used as a design input parameter. Although a rod type TDR (Time Domain Reflectometry) system has been recently proposed to overcome some shortcomings of the conventional methods (particularly the sand cone test), it requires driving the probes into the ground, thus implying that it is still a time-consuming and destructive testing method. This study aims to develop a new non-destructive TDR system that can rapidly measure the dry density, water content, and elastic modulus of soil on the surface of compacted soil, without disturbing the ground. In this study, the Piezoelectric Stack, which is an instrument for measuring the elastic modulus of soil, has been added to the TDR system with a flat type probe, leading to a non-destructive TDR system that is capable of measuring the dry density, water content, and elastic modulus of soil. The new TDR system developed is light enough for an engineer to carry. Results of the standard compaction and TDR tests on sand showed that the dry densities and the moisture contents measured with the new TDR system were in good agreement with those measured with the standard compaction test, respectively. Consequently, it appears that the new TDR system developed will be very useful to advance the current practice of compaction quality control. PMID:28773563

A New Non-Destructive TDR System Combined with a Piezoelectric Stack for Measuring Properties of Geomaterials.

PubMed

Choi, Chanyong; Song, Minwoo; Kim, Daehyeon; Yu, Xiong

2016-06-02

Dry density and water content are two important factors affecting the degree of soil compaction. Conventional methods such as the sand cone test and the plate load test are used to measure such properties for evaluating the degree of compaction and the stiffness of soil in the field. However, these tests are generally very time-consuming and are inherent with some errors depending on the operator (in particular for the sand cone test). Elastic modulus is an indicator to describe the stress-strain behavior of soil and in some cases is used as a design input parameter. Although a rod type TDR (Time Domain Reflectometry) system has been recently proposed to overcome some shortcomings of the conventional methods (particularly the sand cone test), it requires driving the probes into the ground, thus implying that it is still a time-consuming and destructive testing method. This study aims to develop a new non-destructive TDR system that can rapidly measure the dry density, water content, and elastic modulus of soil on the surface of compacted soil, without disturbing the ground. In this study, the Piezoelectric Stack, which is an instrument for measuring the elastic modulus of soil, has been added to the TDR system with a flat type probe, leading to a non-destructive TDR system that is capable of measuring the dry density, water content, and elastic modulus of soil. The new TDR system developed is light enough for an engineer to carry. Results of the standard compaction and TDR tests on sand showed that the dry densities and the moisture contents measured with the new TDR system were in good agreement with those measured with the standard compaction test, respectively. Consequently, it appears that the new TDR system developed will be very useful to advance the current practice of compaction quality control.

Non-destructive investigation of thermoplastic reinforced composites

DOE PAGES

Hassen, Ahmed; Taheri, Hossein; Vaidya, Uday

2016-05-09

This paper studies various manufacturing defects in glass fiber/Polypropylene (PP) composite parts and their methods of detection. Foreign Object Inclusion (FOI) of different shapes, sizes, and materials were placed in a glass fiber/PP panel made by compression molding. The paper aims to characterize the fiber orientation and fiber related defects such as fiber waviness in the composite specimen. Comprehensive investigation for different Non Destructive Evaluation (NDE) techniques, namely X-ray radiography and Ultrasonic Testing (UT) techniques to trace and characterize the embedded defects and the composite texture are presented. Conventional X-ray radiography successfully identified the fiber orientation in two dimension (2-D)more » plane; however, information for the sample depth was not captured. The radiography techniques showed low relative errors for the defect size measurements (maximum error was below 9.5%) when compared to the ultrasonic techniques. Ultrasonic techniques were able to map all the embedded artificial defects. Phase Array (PA) ultrasonic technique was able to precisely locate the FOI in the glass fiber/PP specimen. Nerveless, the shape and size of the defects were not accurately determined due to the high signal attenuation and distortion characteristics of the E-glass fiber.« less

Non-destructive inspection in industrial equipment using robotic mobile manipulation

NASA Astrophysics Data System (ADS)

Maurtua, Iñaki; Susperregi, Loreto; Ansuategui, Ander; Fernández, Ane; Ibarguren, Aitor; Molina, Jorge; Tubio, Carlos; Villasante, Cristobal; Felsch, Torsten; Pérez, Carmen; Rodriguez, Jorge R.; Ghrissi, Meftah

2016-05-01

MAINBOT project has developed service robots based applications to autonomously execute inspection tasks in extensive industrial plants in equipment that is arranged horizontally (using ground robots) or vertically (climbing robots). The industrial objective has been to provide a means to help measuring several physical parameters in multiple points by autonomous robots, able to navigate and climb structures, handling non-destructive testing sensors. MAINBOT has validated the solutions in two solar thermal plants (cylindrical-parabolic collectors and central tower), that are very demanding from mobile manipulation point of view mainly due to the extension (e.g. a thermal solar plant of 50Mw, with 400 hectares, 400.000 mirrors, 180 km of absorber tubes, 140m height tower), the variability of conditions (outdoor, day-night), safety requirements, etc. Once the technology was validated in simulation, the system was deployed in real setups and different validation tests carried out. In this paper two of the achievements related with the ground mobile inspection system are presented: (1) Autonomous navigation localization and planning algorithms to manage navigation in huge extensions and (2) Non-Destructive Inspection operations: thermography based detection algorithms to provide automatic inspection abilities to the robots.

Non-destructive, high-content analysis of wheat grain traits using X-ray micro computed tomography.

PubMed

Hughes, Nathan; Askew, Karen; Scotson, Callum P; Williams, Kevin; Sauze, Colin; Corke, Fiona; Doonan, John H; Nibau, Candida

2017-01-01

Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain uniformity a commercially desired trait, but their analysis is laborious and often requires destructive harvest. One of the current challenges is to develop an accurate, non-destructive method for spike and grain trait analysis capable of handling large populations. In this study we describe the development of a robust method for the accurate extraction and measurement of spike and grain morphometric parameters from images acquired by X-ray micro-computed tomography (μCT). The image analysis pipeline developed automatically identifies plant material of interest in μCT images, performs image analysis, and extracts morphometric data. As a proof of principle, this integrated methodology was used to analyse the spikes from a population of wheat plants subjected to high temperatures under two different water regimes. Temperature has a negative effect on spike height and grain number with the middle of the spike being the most affected region. The data also confirmed that increased grain volume was correlated with the decrease in grain number under mild stress. Being able to quickly measure plant phenotypes in a non-destructive manner is crucial to advance our understanding of gene function and the effects of the environment. We report on the development of an image analysis pipeline capable of accurately and reliably extracting spike and grain traits from crops without the loss of positional information. This methodology was applied to the analysis of wheat spikes can be readily applied to other economically important crop species.

Non-destructive Magnetic Evaluation of Laser Weld Quality in Hot Rolled Coils

NASA Astrophysics Data System (ADS)

Mohapatra, J. N.; Chakradhar, I.; Rao, K. R. C.; Rao, V. V. L.; Kaza, Marutiram

2015-06-01

Weld quality evaluation was conducted on laser welded thin sectsions (2 mm) of hot-rolled (HR) low-carbon steel coils during cold rolling process. The analysis revealed that the poor welds consisting of the weld defects like incomplete fusion, cluster of porosity, and large difference in hardness between the weld zone and base metal were responsible for the weld failures. Experiments were conducted by varying the welding parameters; laser power and welding speed to optimize the parameters for minimizing the weld defects. The optimized weld process parameters have helped elimination of weld defects and the results are verified with microscopy and microhardness measurements. As destructive evaluation techniques are time consuming and not always permitted in industrial applications, attempts have been made in the present investigation for the utilization of suitable non-destructive techniques for the evaluation of weld quality. Non-destructive magnetic techniques of magnetic hysteresis loop and magnetic Barkhausen emissions were used in the present investigation to establish possible correlations of magnetic properties across the weld seam with the mechanical property (microhardness) for evaluation of weld quality. It is inferred that the magnetic properties of coercivity and inverse of root mean square voltage can be effectively utilized to determine weld quality in HR steel coils.

A Monte Carlo approach applied to ultrasonic non-destructive testing

NASA Astrophysics Data System (ADS)

Mosca, I.; Bilgili, F.; Meier, T.; Sigloch, K.

2012-04-01

Non-destructive testing based on ultrasound allows us to detect, characterize and size discrete flaws in geotechnical and architectural structures and materials. This information is needed to determine whether such flaws can be tolerated in future service. In typical ultrasonic experiments, only the first-arriving P-wave is interpreted, and the remainder of the recorded waveform is neglected. Our work aims at understanding surface waves, which are strong signals in the later wave train, with the ultimate goal of full waveform tomography. At present, even the structural estimation of layered media is still challenging because material properties of the samples can vary widely, and good initial models for inversion do not often exist. The aim of the present study is to combine non-destructive testing with a theoretical data analysis and hence to contribute to conservation strategies of archaeological and architectural structures. We analyze ultrasonic waveforms measured at the surface of a variety of samples, and define the behaviour of surface waves in structures of increasing complexity. The tremendous potential of ultrasonic surface waves becomes an advantage only if numerical forward modelling tools are available to describe the waveforms accurately. We compute synthetic full seismograms as well as group and phase velocities for the data. We invert them for the elastic properties of the sample via a global search of the parameter space, using the Neighbourhood Algorithm. Such a Monte Carlo approach allows us to perform a complete uncertainty and resolution analysis, but the computational cost is high and increases quickly with the number of model parameters. Therefore it is practical only for defining the seismic properties of media with a limited number of degrees of freedom, such as layered structures. We have applied this approach to both synthetic layered structures and real samples. The former contributed to benchmark the propagation of ultrasonic surface

Non-destructive analysis of DU content in the NIF hohlraums

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gharibyan, Narek; Moody, Ken J.; Shaughnessy, Dawn A.

2015-12-16

The advantage of using depleted uranium (DU) hohlraums in high-yield deuterium-tritium (DT) shots at the National Ignition Facility (NIF) is addressed by Döppner, et al., in great detail [1]. This DU based hohlraum incorporates a thin layer of DU, ~7 μm thick, on the inner surface along with a thin layer of a gold coating, ~0.7 μm thick, while the outer layer is ~22 μm thick gold. A thickness measurement of the DU layer can be performed using an optical microscope where the total DU weight can be computed provided a uniform DU layer. However, the uniformity of the thicknessmore » is not constant throughout the hohlraum since CAD drawing calculations of the DU weight do not agree with the computed values from optical measurements [2]. Therefore, a non-destructive method for quantifying the DU content in hohlraums has been established by utilizing gamma-ray spectroscopy. The details of this method, along with results from several hohlraums, are presented in this report.« less

Checking collagen preservation in archaeological bone by non-destructive studies (Micro-CT and IBA)

NASA Astrophysics Data System (ADS)

Beck, L.; Cuif, J.-P.; Pichon, L.; Vaubaillon, S.; Dambricourt Malassé, A.; Abel, R. L.

2012-02-01

The material to be studied is a piece of human skull discovered (1999) in Pleistocene sediments from the Orsang river (Gujarat state, India). From anatomical view point, this skull is highly composite: modern Homo sapiens characters are associated to undoubtedly more ancient features. Absolute dating by 14C is critical to understand this discovery. Prior to dating measurements, non-destructive studies have been carried out. Micro-CT reconstruction (X-ray microtomography) and Ion Beam Analysis (IBA) have been undertaken to check the structural preservation of the fossil and the collagen preservation. PIXE elemental map was used to select well-preserved bone area. RBS/EBS and NRA were used for light element quantification, in particular C, N and O contents. We also demonstrate that the PIXE-RBS/EBS combination is a effective tool for the whole characterization of archaeological and recent bones by analysing in one experiment both mineral and organic fractions. We have shown that the archaeological bone, a fragment of the potentially oldest modern Indian, is enough preserved for radiocarbon dating. We propose that Elastic Backscattering Spectrometry (EBS) using 3 MeV protons could be a good non destructive alternative to conventional CHN method using Carbon-Hydrogen-Nitrogen analyzer for measuring C and N before 14C dating.

Non-Enzymatic Detection of Bacterial Genomic DNA Using the Bio-Barcode Assay

PubMed Central

Hill, Haley D.; Vega, Rafael A.; Mirkin, Chad A.

2011-01-01

The detection of bacterial genomic DNA through a non-enzymatic nanomaterials based amplification method, the bio-barcode assay, is reported. The assay utilizes oligonucleotide functionalized magnetic microparticles to capture the target of interest from the sample. A critical step in the new assay involves the use of blocking oligonucleotides during heat denaturation of the double stranded DNA. These blockers bind to specific regions of the target DNA upon cooling, and prevent the duplex DNA from re-hybridizing, which allows the particle probes to bind. Following target isolation using the magnetic particles, oligonucleotide functionalized gold nanoparticles act as target recognition agents. The oligonucleotides on the nanoparticle (barcodes) act as amplification surrogates. The barcodes are then detected using the Scanometric method. The limit of detection for this assay was determined to be 2.5 femtomolar, and this is the first demonstration of a barcode type assay for the detection of double stranded, genomic DNA. PMID:17927207

Fast solver for large scale eddy current non-destructive evaluation problems

NASA Astrophysics Data System (ADS)

Lei, Naiguang

Eddy current testing plays a very important role in non-destructive evaluations of conducting test samples. Based on Faraday's law, an alternating magnetic field source generates induced currents, called eddy currents, in an electrically conducting test specimen. The eddy currents generate induced magnetic fields that oppose the direction of the inducing magnetic field in accordance with Lenz's law. In the presence of discontinuities in material property or defects in the test specimen, the induced eddy current paths are perturbed and the associated magnetic fields can be detected by coils or magnetic field sensors, such as Hall elements or magneto-resistance sensors. Due to the complexity of the test specimen and the inspection environments, the availability of theoretical simulation models is extremely valuable for studying the basic field/flaw interactions in order to obtain a fuller understanding of non-destructive testing phenomena. Theoretical models of the forward problem are also useful for training and validation of automated defect detection systems. Theoretical models generate defect signatures that are expensive to replicate experimentally. In general, modelling methods can be classified into two categories: analytical and numerical. Although analytical approaches offer closed form solution, it is generally not possible to obtain largely due to the complex sample and defect geometries, especially in three-dimensional space. Numerical modelling has become popular with advances in computer technology and computational methods. However, due to the huge time consumption in the case of large scale problems, accelerations/fast solvers are needed to enhance numerical models. This dissertation describes a numerical simulation model for eddy current problems using finite element analysis. Validation of the accuracy of this model is demonstrated via comparison with experimental measurements of steam generator tube wall defects. These simulations generating two

An ELISA DYRK1A non-radioactive kinase assay suitable for the characterization of inhibitors

PubMed Central

Liu, Yong; Adayev, Tatyana; Hwang, Yu-Wen

2017-01-01

The DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1A) gene encodes a proline-directed Ser/Thr kinase. Elevated expression and/or altered distribution of the kinase have been implicated in the neurological impairments associated with Down syndrome (DS) and Alzheimer’s disease (AD). Consequently, DYRK1A inhibition has been of significant interest as a potential strategy for therapeutic intervention of DS and AD. Many classes of novel inhibitors have been described in the past decade. Although non-radioactive methods for analyzing DYRK1A inhibition have been developed, methods employing radioactive tracers are still commonly used for quantitative characterization of DYRK1A inhibitors. Here, we present a non-radioactive ELISA assay based on the detection of DYRK1A-phosphorylated dynamin 1a fragment using a phosphorylation site-specific antibody. The assay was verified by the use of two well-characterized DYRK1A inhibitors, epigallocatechin gallate (EGCG) and harmine. The IC 50s for EGCG and harmine determined by the ELISA method were found to be comparable to those previously measured by radioactive tracing methods.  Furthermore, we determined the mode of inhibition for EGCG and harmine by a modification of the ELISA assay. This assay confirms the mode of inhibition of EGCG (non-ATP-competitive) and harmine (ATP-competitive), as previously determined. We conclude that the ELISA platform demonstrated here is a viable alternative to the traditional radioactive tracer assays for analyzing DYRK1A inhibitors. PMID:28163906

Non-Destructive Evaluation of Polyolefin Thermal Aging Using Infrared Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fifield, Leonard S.; Shin, Yongsoon; Simmons, Kevin L.

Fourier transform infrared (FTIR) spectroscopy is an information-rich method that reveals chemical bonding near the surface of polymer composites. FTIR can be used to verify composite composition, identify chemical contaminants and expose composite moisture content. Polymer matrix changes due to thermal exposure including loss of additives, chain scission, oxidation and changes in crystallinity may also be determined using FTIR spectra. Portable handheld instruments using non-contact reflectance or surface contact attenuated total reflectance (ATR) may be used for non-destructive evaluation (NDE) of thermal aging in polymer and composite materials of in-service components. We report the use of ATR FTIR to trackmore » oxidative thermal aging in ethylene-propylene rubber (EPR) and chlorinated polyethylene (CPE) materials used in medium voltage nuclear power plant electrical cable insulation and jacketing. Mechanical property changes of the EPR and CPE materials with thermal degradation for correlation with FTIR data are tracked using indenter modulus (IM) testing. IM is often used as a local NDE metric of cable jacket health. The FTIR-determined carbonyl index was found to increase with IM and may be a valuable NDE metric with advantages over IM for assessing cable remaining useful life.« less

Non-Destructive Spectroscopic Techniques and Multivariate Analysis for Assessment of Fat Quality in Pork and Pork Products: A Review

PubMed Central

Kucha, Christopher T.; Liu, Li; Ngadi, Michael O.

2018-01-01

Fat is one of the most important traits determining the quality of pork. The composition of the fat greatly influences the quality of pork and its processed products, and contribute to defining the overall carcass value. However, establishing an efficient method for assessing fat quality parameters such as fatty acid composition, solid fat content, oxidative stability, iodine value, and fat color, remains a challenge that must be addressed. Conventional methods such as visual inspection, mechanical methods, and chemical methods are used off the production line, which often results in an inaccurate representation of the process because the dynamics are lost due to the time required to perform the analysis. Consequently, rapid, and non-destructive alternative methods are needed. In this paper, the traditional fat quality assessment techniques are discussed with emphasis on spectroscopic techniques as an alternative. Potential spectroscopic techniques include infrared spectroscopy, nuclear magnetic resonance and Raman spectroscopy. Hyperspectral imaging as an emerging advanced spectroscopy-based technology is introduced and discussed for the recent development of assessment for fat quality attributes. All techniques are described in terms of their operating principles and the research advances involving their application for pork fat quality parameters. Future trends for the non-destructive spectroscopic techniques are also discussed. PMID:29382092

Local destruction of superconductivity by non-magnetic impurities in mesoscopic iron-based superconductors

PubMed Central

Li, Jun; Ji, Min; Schwarz, Tobias; Ke, Xiaoxing; Van Tendeloo, Gustaaf; Yuan, Jie; Pereira, Paulo J.; Huang, Ya; Zhang, Gufei; Feng, Hai-Luke; Yuan, Ya-Hua; Hatano, Takeshi; Kleiner, Reinhold; Koelle, Dieter; Chibotaru, Liviu F.; Yamaura, Kazunari; Wang, Hua-Bing; Wu, Pei-Heng; Takayama-Muromachi, Eiji; Vanacken, Johan; Moshchalkov, Victor V.

2015-01-01

The determination of the pairing symmetry is one of the most crucial issues for the iron-based superconductors, for which various scenarios are discussed controversially. Non-magnetic impurity substitution is one of the most promising approaches to address the issue, because the pair-breaking mechanism from the non-magnetic impurities should be different for various models. Previous substitution experiments demonstrated that the non-magnetic zinc can suppress the superconductivity of various iron-based superconductors. Here we demonstrate the local destruction of superconductivity by non-magnetic zinc impurities in Ba0.5K0.5Fe2As2 by exploring phase-slip phenomena in a mesoscopic structure with 119 × 102 nm2 cross-section. The impurities suppress superconductivity in a three-dimensional ‘Swiss cheese'-like pattern with in-plane and out-of-plane characteristic lengths slightly below ∼1.34 nm. This causes the superconducting order parameter to vary along abundant narrow channels with effective cross-section of a few square nanometres. The local destruction of superconductivity can be related to Cooper pair breaking by non-magnetic impurities. PMID:26139568

Non-destructive digital imaging in poplar allows detailed analysis of adventitious rooting dynamics

Treesearch

R.J. Kodrzycki; R.B. Michaels; A.L. Friend; R.S. Zalesny; Ch.P. Mawata; D.W. McDonald

2008-01-01

The dynamics of root formation are difficult to observe directly over time without disturbing the rooting environment. A novel system for a non-destructive, non-invasive root analysis (RootViz FS, Phenotype Screening Corp.) was evaluated for its ability to analyze root formation from cuttings over a 32 day period in three poplar genotypes (DN70, P. Deltoides x...

Non-destructive automated sampling of mycotoxins in bulk food and feed - A new tool for required harmonization.

PubMed

Spanjer, M; Stroka, J; Patel, S; Buechler, S; Pittet, A; Barel, S

2001-06-01

Mycotoxins contamination is highly non-uniformly distributed as is well recog-nized by the EC, by not only setting legal limits in a series of commodities, but also schedule a sampling plan that takes this heterogeneity into account. In practice however, it turns out that it is very difficult to carry out this sampling plan in a harmonised way. Applying the sampling plan to a container filled with pallets of bags (i.e. with nuts or coffee beans) varies from very laborious to almost impossible. The presented non-destructive automated method to sample bulk food could help to overcome these practical problems and to enforcing of EC directives. It is derived from a tested and approved technology for detection of illicit substances in security applications. It has capability to collect and iden-tify ultra trace contaminants, i.e. from a fingerprint of chemical substance in a bulk of goods, a cargo pallet load (~ 1000 kg) with boxes and commodities.The technology, patented for explosives detection, uses physical and chemistry processes for excitation and remote rapid enhanced release of contaminant residues, vapours and particulate, of the inner/outer surfaces of inspected bulk and collect them on selective probes. The process is automated, takes only 10 minutes, is non-destructive and the bulk itself remains unharmed. The system design is based on applicable international regulations for shipped cargo hand-ling and transportation by road, sea and air. After this process the pallet can be loaded on a truck, ship or plane. Analysis can be carried out before the cargo leaves the place of shipping. The potent application of this technology for myco-toxins detection, has been demonstrated by preliminary feasibility experiments. Aflatoxins were detected in pistachios and ochratoxin A in green coffee beans bulk. Both commodities were naturally contaminated, priory found and confirm-ed by common methods as used at routine inspections. Once the contaminants are extracted from a

Intermodal transportation infrastructure interactions : utilizing acoustic emission and other non-destructive evaluation technologies.

DOT National Transportation Integrated Search

2014-09-01

This project studied application of acoustic emission (AE) technology to perform structural : health monitoring of highway bridges. Highway bridges are a vital part of transportation : infrastructure and there is need for reliable non-destructive met...

Trends of non-destructive analytical methods for identification of biodiesel feedstock in diesel-biodiesel blend according to European Commission Directive 2012/0288/EC and detecting diesel-biodiesel blend adulteration: A brief review.

PubMed

Mazivila, Sarmento Júnior

2018-04-01

Discrimination of biodiesel feedstock present in diesel-biodiesel blend is challenging due to the great similarity in the spectral profile as well as digital image profile of each type of feedstock employed in biodiesel production. Once the marketed diesel-biodiesel blend is subsidized, in which motivates adulteration in biofuel blend by cheaper supplies with high solubility to obtain profits associated with the subsidies involved in biodiesel production. Non-destructive analytical methods based on qualitative and quantitative analysis for detecting marketed diesel-biodiesel blend adulteration are reviewed. Therefore, at the end is discussed the advantage of the qualitative analysis over quantitative analysis, when the systems require immediate decisions such as to know if the marketed diesel-biodiesel blend is unadulterated or adulterated in order to aid the analyst in selecting the most appropriate green analytical procedure for detecting diesel-biodiesel blend adulteration proceeding in fast way. This critical review provides a brief review on the non-destructive analytical methods reported in scientific literature based on different first-order multivariate calibration models coupled with spectroscopy data and digital image data to identify the type of biodiesel feedstock present in diesel-biodiesel blend in order to meets the strategies adopted by European Commission Directive 2012/0288/EC as well as to monitoring diesel-biodiesel adulteration. According to that Directive, from 2020 biodiesel produced from first-generation feedstock, that is, oils employed in human food such as sunflower, soybean, rapeseed, palm oil, among other oils should not be subsidized. Therefore, those non-destructive analytical methods here reviewed are helpful for discrimination of biodiesel feedstock present in diesel-biodiesel blend according to European Commission Directive 2012/0288/EC as well as for detecting diesel-biodiesel blend adulteration. Copyright © 2017 Elsevier B

Monitoring fungal growth on brown rice grains using rapid and non-destructive hyperspectral imaging.

PubMed

Siripatrawan, U; Makino, Y

2015-04-16

This research aimed to develop a rapid, non-destructive, and accurate method based on hyperspectral imaging (HSI) for monitoring spoilage fungal growth on stored brown rice. Brown rice was inoculated with a non-pathogenic strain of Aspergillus oryzae and stored at 30 °C and 85% RH. Growth of A. oryzae on rice was monitored using viable colony counts, expressed as colony forming units per gram (CFU/g). The fungal development was observed using scanning electron microscopy. The HSI system was used to acquire reflectance images of the samples covering the visible and near-infrared (NIR) wavelength range of 400-1000 nm. Unsupervised self-organizing map (SOM) was used to visualize data classification of different levels of fungal infection. Partial least squares (PLS) regression was used to predict fungal growth on rice grains from the HSI reflectance spectra. The HSI spectral signals decreased with increasing colony counts, while conserving similar spectral pattern during the fungal growth. When integrated with SOM, the proposed HSI method could be used to classify rice samples with different levels of fungal infection without sample manipulation. Moreover, HSI was able to rapidly identify infected rice although the samples showed no symptoms of fungal infection. Based on PLS regression, the coefficient of determination was 0.97 and root mean square error of prediction was 0.39 log (CFU/g), demonstrating that the HSI technique was effective for prediction of fungal infection in rice grains. The ability of HSI to detect fungal infection at early stage would help to prevent contaminated rice grains from entering the food chain. This research provides scientific information on the rapid, non-destructive, and effective fungal detection system for rice grains. Copyright © 2015 Elsevier B.V. All rights reserved.

Giant Magnetoresistance Sensors: A Review on Structures and Non-Destructive Eddy Current Testing Applications

PubMed Central

Rifai, Damhuji; Abdalla, Ahmed N.; Ali, Kharudin; Razali, Ramdan

2016-01-01

Non-destructive eddy current testing (ECT) is widely used to examine structural defects in ferromagnetic pipe in the oil and gas industry. Implementation of giant magnetoresistance (GMR) sensors as magnetic field sensors to detect the changes of magnetic field continuity have increased the sensitivity of eddy current techniques in detecting the material defect profile. However, not many researchers have described in detail the structure and issues of GMR sensors and their application in eddy current techniques for nondestructive testing. This paper will describe the implementation of GMR sensors in non-destructive testing eddy current testing. The first part of this paper will describe the structure and principles of GMR sensors. The second part outlines the principles and types of eddy current testing probe that have been studied and developed by previous researchers. The influence of various parameters on the GMR measurement and a factor affecting in eddy current testing will be described in detail in the third part of this paper. Finally, this paper will discuss the limitations of coil probe and compensation techniques that researchers have applied in eddy current testing probes. A comprehensive review of previous studies on the application of GMR sensors in non-destructive eddy current testing also be given at the end of this paper. PMID:26927123

Giant Magnetoresistance Sensors: A Review on Structures and Non-Destructive Eddy Current Testing Applications.

PubMed

Rifai, Damhuji; Abdalla, Ahmed N; Ali, Kharudin; Razali, Ramdan

2016-02-26

Non-destructive eddy current testing (ECT) is widely used to examine structural defects in ferromagnetic pipe in the oil and gas industry. Implementation of giant magnetoresistance (GMR) sensors as magnetic field sensors to detect the changes of magnetic field continuity have increased the sensitivity of eddy current techniques in detecting the material defect profile. However, not many researchers have described in detail the structure and issues of GMR sensors and their application in eddy current techniques for nondestructive testing. This paper will describe the implementation of GMR sensors in non-destructive testing eddy current testing. The first part of this paper will describe the structure and principles of GMR sensors. The second part outlines the principles and types of eddy current testing probe that have been studied and developed by previous researchers. The influence of various parameters on the GMR measurement and a factor affecting in eddy current testing will be described in detail in the third part of this paper. Finally, this paper will discuss the limitations of coil probe and compensation techniques that researchers have applied in eddy current testing probes. A comprehensive review of previous studies on the application of GMR sensors in non-destructive eddy current testing also be given at the end of this paper.

Evaluation of stress-induced martensite phase in ferromagnetic shape memory alloy Fe-30.2at%Pd by non-destructive Barkhausen noise

NASA Astrophysics Data System (ADS)

Furuya, Yasubumi; Okazaki, Teiko; Ueno, Takasi; Spearing, Mark; Wutting, Manfred

2005-05-01

Barkhausen noise (BHN) method seems a useful tecnique to non-destructive evaluation of martensite phase transformation of ferromagnetic shape memory alloy, which is used as the filler of our proposing "Smart Composite Board". The concept of design for "Smart Composite Board" which can combine the non-destructive magnetic inspection and shape recovery function in the material itself was formerly proposed. In the present study, we survey the possibility of Barkhausen noise (BHN) method to detect the transformation of microscopic martensite phase caused by stress-loading in Fe-30.2at%Pd thin foil, which has a stable austenite phase (fcc structure) at room temperature. The BHN voltage was measured at loading stress up to 100 MPa in temperature range of 300K to 373K. Stress-induced martensite twin was observed by laser microscope above loading stress of 25 MPa. A phase transformation caused by loading stress were analyzed also by X-ray diffraction. The signals of BHN are analyzed by the time of magnetization and the noise frequency. BHN caused by grain boundaries appears in the lower frequency range (1kHz-3kHz) and BHN by martensite twin in the higher frequency range (8kHz-10kHz). The envelope of the BHN voltage as a function of time of magnetization shows a peak due to austenite phase at weak magnetic field. The BHN envelope due to martensite twins creates additional two peaks at intermediate magnetic field. BHN method turns out to be a powerful technique for non-destructive evaluation of the phase transformation of ferromagnetic shape memory alloy.

Augmented reality application for industrial non-destructive inspection training

NASA Astrophysics Data System (ADS)

Amza, Catalin Gheorghe; Zapciu, Aurelian; Teodorescu, Octav

2018-02-01

Such a technology - Augmented Reality (AR) has great potential of use, especially for training purposes of new operators on using expensive equipment. In this context, the paper presents an augmented reality training system developed for phased-array ultrasonic non-destructive testing (NDT) equipment. The application has been developed using Unity 5.6.0 game-engine platform integrated with Vuforia sdk toolkit for devices with Android operating system. The test results performed by several NDT operators showed good results, thus proving the potential of using the application in the industrial field.

Non-Destructive Evaluation of Depth of Surface Cracks Using Ultrasonic Frequency Analysis

PubMed Central

Her, Shiuh-Chuan; Lin, Sheng-Tung

2014-01-01

Ultrasonic is one of the most common uses of a non-destructive evaluation method for crack detection and characterization. The effectiveness of the acoustic-ultrasound Structural Health Monitoring (SHM) technique for the determination of the depth of the surface crack was presented. A method for ultrasonic sizing of surface cracks combined with the time domain and frequency spectrum was adopted. The ultrasonic frequency spectrum was obtained by Fourier transform technique. A series of test specimens with various depths of surface crack ranging from 1 mm to 8 mm was fabricated. The depth of the surface crack was evaluated using the pulse-echo technique. In this work, three different longitudinal waves with frequencies of 2.25 MHz, 5 MHz and 10 MHz were employed to investigate the effect of frequency on the sizing detection of surface cracks. Reasonable accuracies were achieved with measurement errors less than 7%. PMID:25225875

Usage of FTIR-ATR as Non-Destructive Analysis of Selected Toxic Dyes

NASA Astrophysics Data System (ADS)

Bartošová, Alica; Blinová, Lenka; Sirotiak, Maroš; Michalíková, Anna

2017-06-01

The degradation of the environment which is due to the discharge of polluting wastewater from industrial sources poses a real problem in several countries. Textile industries use large volumes of water in their operations, discharging thus large volume of wastewater into the environment, most of which is untreated. The wastewater contains a variety of chemicals from various stages of process operations, including desizing, scouring, bleaching and dyeing. The main purpose of this paper is to introduce Infrared Spectrometry with Fourier transformation as a non-destructive method for study, identifation and rapid determination of selected representatives of cationic (Methylene Blue), azo (Congo Red, Eriochrome Black T) and nitroso (Naphthol Green B) dyes. In conjunction with the ATR technique, FTIR offers a reliable detection method of dyes without extraction by other dangerous substances. Spectral interpretation of dye spectra revealed valuable information about the identification and characterization of each group of dyes.

Analysis of Within-Test Variability of Non-Destructive Test Methods to Evaluate Compressive Strength of Normal Vibrated and Self-Compacting Concretes

NASA Astrophysics Data System (ADS)

Nepomuceno, Miguel C. S.; Lopes, Sérgio M. R.

2017-10-01

Non-destructive tests (NDT) have been used in the last decades for the assessment of in-situ quality and integrity of concrete elements. An important step in the application of NDT methods concerns to the interpretation and validation of the test results. In general, interpretation of NDT results should involve three distinct phases leading to the development of conclusions: processing of collected data, analysis of within-test variability and quantitative evaluation of property under investigation. The analysis of within-test variability can provide valuable information, since this can be compared with that of within-test variability associated with the NDT method in use, either to provide a measure of the quality control or to detect the presence of abnormal circumstances during the in-situ application. This paper reports the analysis of the experimental results of within-test variability of NDT obtained for normal vibrated concrete and self-compacting concrete. The NDT reported includes the surface hardness test, ultrasonic pulse velocity test, penetration resistance test, pull-off test, pull-out test and maturity test. The obtained results are discussed and conclusions are presented.

Rapid and non-destructive assessment of polyunsaturated fatty acids contents in Salmon using near-infrared hyperspectral imaging

NASA Astrophysics Data System (ADS)

Tao, Feifei; Mba, Ogan; Liu, Li; Ngadi, Michael

2017-04-01

Polyunsaturated fatty acids (PUFAs) are important nutrients present in Salmon. However, current methods for quantifying the fatty acids (FAs) contents in foods are generally based on gas chromatography (GC) technique, which is time-consuming, laborious and destructive to the tested samples. Therefore, the capability of near-infrared (NIR) hyperspectral imaging to predict the PUFAs contents of C20:2 n-6, C20:3 n-6, C20:5 n-3, C22:5 n-3 and C22:6 n-3 in Salmon fillets in a rapid and non-destructive way was investigated in this work. Mean reflectance spectra were first extracted from the region of interests (ROIs), and then the spectral pre-processing methods of 2nd derivative and Savitzky-Golay (SG) smoothing were performed on the original spectra. Based on the original and the pre-processed spectra, PLSR technique was employed to develop the quantitative models for predicting each PUFA content in Salmon fillets. The results showed that for all the studied PUFAs, the quantitative models developed using the pre-processed reflectance spectra by "2nd derivative + SG smoothing" could improve their modeling results. Good prediction results were achieved with RP and RMSEP of 0.91 and 0.75 mg/g dry weight, 0.86 and 1.44 mg/g dry weight, 0.82 and 3.01 mg/g dry weight for C20:3 n-6, C22:5 n-3 and C20:5 n-3, respectively after pre-processing by "2nd derivative + SG smoothing". The work demonstrated that NIR hyperspectral imaging could be a useful tool for rapid and non-destructive determination of the PUFA contents in fish fillets.

Recent advances in rapid and non-destructive assessment of meat quality using hyperspectral imaging

NASA Astrophysics Data System (ADS)

Tao, Feifei; Ngadi, Michael

2016-05-01

Meat is an important food item in human diet. Its production and consumption has greatly increased in the last decades with the development of economies and improvement of peoples' living standards. However, most of the traditional methods for evaluation of meat quality are time-consuming, laborious, inconsistent and destructive to samples, which make them not appropriate for a fast-paced production and processing environment. Development of innovative and non-destructive optical sensing techniques to facilitate simple, fast, and accurate evaluation of quality are attracting increasing attention in the food industry. Hyperspectral imaging is one of the promising techniques. It integrates the combined merits of imaging and spectroscopic techniques. This paper provides a comprehensive review on recent advances in evaluation of the important quality attributes of meat including color, marbling, tenderness, pH, water holding capacity, and also chemical composition attributes such as moisture content, protein content and fat content in pork, beef and lamb. In addition, the future potential applications and trends of hyperspectral imaging are also discussed in this paper.

Non-destructive 3D shape measurement of transparent and black objects with thermal fringes

NASA Astrophysics Data System (ADS)

Brahm, Anika; Rößler, Conrad; Dietrich, Patrick; Heist, Stefan; Kühmstedt, Peter; Notni, Gunther

2016-05-01

Fringe projection is a well-established optical method for the non-destructive contactless three-dimensional (3D) measurement of object surfaces. Typically, fringe sequences in the visible wavelength range (VIS) are projected onto the surfaces of objects to be measured and are observed by two cameras in a stereo vision setup. The reconstruction is done by finding corresponding pixels in both cameras followed by triangulation. Problems can occur if the properties of some materials disturb the measurements. If the objects are transparent, translucent, reflective, or strongly absorbing in the VIS range, the projected patterns cannot be recorded properly. To overcome these challenges, we present a new alternative approach in the infrared (IR) region of the electromagnetic spectrum. For this purpose, two long-wavelength infrared (LWIR) cameras (7.5 - 13 μm) are used to detect the emitted heat radiation from surfaces which is induced by a pattern projection unit driven by a CO2 laser (10.6 μm). Thus, materials like glass or black objects, e.g. carbon fiber materials, can be measured non-destructively without the need of any additional paintings. We will demonstrate the basic principles of this heat pattern approach and show two types of 3D systems based on a freeform mirror and a GOBO wheel (GOes Before Optics) projector unit.

Non-destructive Evaluation of Bonds Between Fiberglass Composite and Metal

NASA Technical Reports Server (NTRS)

Zhao, Selina; Sonta, Kestutis; Perey, Daniel F.; Cramer, K. E.; Berger, Libby

2015-01-01

To assess the integrity and reliability of an adhesive joint in an automotive composite component, several non-destructive evaluation (NDE) methodologies are correlated to lap shear bond strengths. A glass-fabric-reinforced composite structure was bonded to a metallic structure with a two-part epoxy adhesive. Samples were subsequently cut and tested in shear, and flaws were found in some areas. This study aims to develop a reliable and portable NDE system for service-level adhesive inspection in the automotive industry. The results of the experimental investigation using several NDE methods are presented and discussed. Fiberglass-to-metal bonding is the ideal configuration for NDE via thermography using excitation with induction heating, due to the conductive metal and non-conductive glass-fiber-reinforced composites. Excitation can be either by a research-grade induction heater of highly defined frequency and intensity, or by a service-level heater, such as would be used for sealing windshields in a body shop. The thermographs thus produced can be captured via a high-resolution infrared camera, with principal component analysis and 2D spatial Laplacian processing. Alternatively, the thermographs can be captured by low resolution thermochromic microencapsulated liquid crystal film imaging, which needs no post-processing and can be very inexpensive. These samples were also examined with phased-array ultrasound. The NDE methods are compared to the lap shear values and to each other for approximate cost, accuracy, and time and level of expertise needed.

Method and Apparatus for Non-Destructive Evaluation of Materials

NASA Technical Reports Server (NTRS)

Washabaugh, Andrew P. (Inventor); Lyons, Robert (Inventor); Thomas, Zachary (Inventor); Martin, Christopher (Inventor); Goldfine, Neil J. (Inventor)

2017-01-01

Methods and apparatus for characterizing composite materials for manufacturing quality assurance (QA), periodic inspection during the useful life, or for forensic analysis/material testing. System are provided that relate eddy-current sensor responses to the fiber layup of a composite structure, the presence of impact damage on a composite structure with or without a metal liner, volumetric stress within the composite, fiber tow density, and other NDE inspection requirements. Also provided are systems that determine electromagnetic material properties and material dimensions of composite materials from capacitive sensor inspection measurements. These properties are related to the presence of buried defects in non-conductive composite materials, moisture ingress, aging of the material due to service or environmental/thermal exposure, or changes in manufacturing quality.

Method and Apparatus for Non-Destructive Evaluation of Materials

NASA Technical Reports Server (NTRS)

Lyons, Robert (Inventor); Martin, Christopher (Inventor); Washabaugh, Andrew P. (Inventor); Goldfine, Neil J. (Inventor); Thomas, Zachary (Inventor); Jablonski, David A. (Inventor)

2015-01-01

Methods and apparatus for characterizing composite materials for manufacturing quality assurance (QA), periodic inspection during the useful life, or for forensic analysis/material testing. System are provided that relate eddy-current sensor responses to the fiber layup of a composite structure, the presence of impact damage on a composite structure with or without a metal liner, volumetric stress within the composite, fiber tow density, and other NDE inspection requirements. Also provided are systems that determine electromagnetic material properties and material dimensions of composite materials from capacitive sensor inspection measurements. These properties are related to the presence of buried defects in non-conductive composite materials, moisture ingress, aging of the material due to service or environmental/thermal exposure, or changes in manufacturing quality.

Spectrofluorimetric assay method for glutathione and glutathione transferase using monobromobimane.

PubMed

Yakubu, S I; Yakasai, I A; Musa, A

2011-06-01

The primary role of glutathione transferase is to defend an organism from toxicities through catalyzing the reaction of glutathione (GSH) with potentially toxic compounds or metabolites to their chemically and biologically inert conjugates. The objective of the study was to develop a simple and sensitive spectrofluorimetric assay method for glutathione transferase using monobromobimane (MBB), a non fluorescent compound with electrophilic site. MBB slowly reacted with glutathione to form fluorescent glutathione conjugate and that the reaction was catalysed by glutathione transferase. Both non-enzymatic and enzymatic reaction products of MBB, in presence of GSH in phosphate buffer (pH 6.5), were measured by following increase of fluorescence at wavelength of 475nm. For validation of the assay method, the kinetic parameters such as the apparent Michaelis-Mente constants and maximum rates of conjugate formation as well as the specific activity of rat hepatic glutathione transferase were determined. The method was found to be sensitive, thus, applied to measure glutathione contents of crude preparation of rat hepatic cytosol fraction.

Non-destructive testing techniques based on nonlinear methods for assessment of debonding in single lap joints

NASA Astrophysics Data System (ADS)

Scarselli, G.; Ciampa, F.; Ginzburg, D.; Meo, M.

2015-04-01

Nonlinear ultrasonic non-destructive evaluation (NDE) methods can be used for the identification of defects within adhesive bonds as they rely on the detection of nonlinear elastic features for the evaluation of the bond strength. In this paper the nonlinear content of the structural response of a single lap joint subjected to ultrasonic harmonic excitation is both numerically and experimentally evaluated to identify and characterize the defects within the bonded region. Different metallic samples with the same geometry were experimentally tested in order to characterize the debonding between two plates by using two surface bonded piezoelectric transducers in pitch-catch mode. The dynamic response of the damaged samples acquired by the single receiver sensor showed the presence of higher harmonics (2nd and 3rd) and subharmonics of the fundamental frequencies. These nonlinear elastic phenomena are clearly due to nonlinear effects induced by the poor adhesion between the two plates. A new constitutive model aimed at representing the nonlinear material response generated by the interaction of the ultrasonic waves with the adhesive joint is also presented. Such a model is implemented in an explicit FE software and uses a nonlinear user defined traction-displacement relationship implemented by means of a cohesive material user model interface. The developed model is verified for the different geometrical and material configurations. Good agreement between the experimental and numerical nonlinear response showed that this model can be used as a simple and useful tool for understanding the quality of the adhesive joint.

Non-destructive evaluation of coating thickness using guided waves

NASA Astrophysics Data System (ADS)

Ostiguy, Pierre-Claude; Quaegebeur, Nicolas; Masson, Patrice

2015-04-01

Among existing strategies for non-destructive evaluation of coating thickness, ultrasonic methods based on the measurement of the Time-of-Flight (ToF) of high frequency bulk waves propagating through the thickness of a structure are widespread. However, these methods only provide a very localized measurement of the coating thickness and the precision on the results is largely affected by the surface roughness, porosity or multi-layered nature of the host structure. Moreover, since the measurement is very local, inspection of large surfaces can be time consuming. This article presents a robust methodology for coating thickness estimation based on the generation and measurement of guided waves. Guided waves have the advantage over ultrasonic bulk waves of being less sensitive to surface roughness, and of measuring an average thickness over a wider area, thus reducing the time required to inspect large surfaces. The approach is based on an analytical multi-layer model and intercorrelation of reference and measured signals. The method is first assessed numerically for an aluminum plate, where it is demonstrated that coating thickness can be measured within a precision of 5 micrometers using the S0 mode at frequencies below 500 kHz. Then, an experimental validation is conducted and results show that coating thicknesses in the range of 10 to 200 micrometers can be estimated within a precision of 10 micrometers of the exact coating thickness on this type of structure.

Non-Destructive Testing with Atmospheric Pressure Radio-Frequency Plasma

NASA Astrophysics Data System (ADS)

May, A.; Andarawis, E.

2007-03-01

We summarize our recent work using radio-frequency (RF) atmospheric pressure plasma (APP) for non-destructive evaluation (NDE), specifically for: (1) Clearance sensing (0-5mm) on rotating components, and (2) Generation of broadband ultrasound in air at 900kHz. RF-APP showed potential in both of these common NDE requirements, but further work is required to better characterize and optimize the performance of the new techniques. Application of RF-APP to other NDE disciplines, such as plasma spectroscopy and gas flow measurement, is also likely to be advantageous, especially in harsh environments where existing approaches are prohibitively expensive or complex.

Potential techniques for non-destructive evaluation of cable materials

NASA Astrophysics Data System (ADS)

Gillen, Kenneth T.; Clough, Roger L.; Mattson, Bengt; Stenberg, Bengt; Oestman, Erik

This paper describes the connection between mechanical degradation of common cable materials, in radiation and elevated temperature environments, and density increases caused by the oxidation which leads to this degradation. Two techniques based on density changes are suggested as potential non-destructive evaluation (NDE) procedures which may be applicable to monitoring the mechanical condition of cable materials in power plant environments. The first technique is direct measurement of density changes, via a density gradient column, using small shavings removed from the surface of cable jackets at selected locations. The second technique is computed X-ray tomography, utilizing a portable scanning device.

High-Energy Laser for Detection, Inspection, and Non-Destructive Testing

DTIC Science & Technology

2011-03-21

at the gra odes. The -2 at 0.1 Hz -cm-2, and mage thre n array of beam. Th burns on pled to a to measur laser is eq micron lev ent beam ( rget...project or resulting research?  Defense Threat Reduction Agency, “Compact Source of Laser -Driven Monoenergetic Gamma-Rays” --$2,982,685... LASER FOR DETECTION, INSPECTION, AND NON-DESTRUCTIVE TESTING 3) Grant/Contract Number: FA9550-07-1-0521 4) Reporting Period Start: 06/21/2007

Variation in gymnemic acid content and non-destructive harvesting of Gymnema sylvestre (Gudmar)

PubMed Central

Pandey, Ashok Kumar; Yadav, Swati

2010-01-01

Background: Madhunashini (Gymnema sylvestre R. Br.) commonly known as ‘Gudmar’ in Hindi is an important medicinal climber and extensively used in almost all Indian System of Medicine as a remedy for diabetes, rheumatism, cough, ulcer, jaundice, dyspepsia, constipation, eyes pain and also in snakebite. In India, it is found growing in Andhra Pradesh, Bihar, Chhattisgarh, Karnataka, Kerala, Madhya Pradesh, Maharastra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal. The major phytoconstituents are gymnemic acids, gudmarin and saponins. Methods: In the present study, Gymnema germplasm collected from various regions of Madhya Pradesh was evaluated on the basis of their morphological characteristics and gymnemic acid content. Gymnemic acid content in the leaves was estimated by HPLC. We have also standardized the non-destructive harvesting practices of Gudmar. Selective harvesting was done without harming the main plant. Only mature leaves (60%) were hand plucked in the month of October. Second harvest was done in the month of June. Results: Data revealed that gymnemic acid content varied between 0.96% ± 0.03 (Seoni) to 1.58% ±0.03 (Amarkantak). It was also observed that the leaves left at the time of 1st harvest during October matured in June at the time of 2nd harvest. Conclusion: Non destructive harvesting practice did not have any negative impact on overall development of the plant. It is evident that there is wide variation in the morphological characteristics and gymnemic acid content in G. sylvestre collected from various locations, which can be exploited for further crop improvement programmes. PMID:21589758

Non-destructive determination of thickness of the dielectric layers using EDX

NASA Astrophysics Data System (ADS)

Sokolov, S. A.; Kelm, E. A.; Milovanov, R. A.; Abdullaev, D. A.; Sidorov, L. N.

2016-12-01

In this work a non-destructive method for measuring the thickness of the dielectric layers consisting of silicon dioxide and silicon nitride has been developed using a scanning electron microscope (SEM) equipped with energy dispersive X-ray spectrometer (EDS). Rising in accelerating voltage of electron beam leads to increasing in the depth of generation of the characteristic X-ray. If the ratio of the signal intensity of one of the substrate's elements to the noise equal to 3 suggests that the generation's depth of the characteristic X-ray coincides with the thickness of the overlying film. Dependence of the overlying film's thickness on the accelerating voltage can be plotted. Validation of the results was carried out by using the equation of Anderson-Hassler. The generation's volume of the characteristic X-Ray was simulated by CASINO program. The simulations results are in good agreement with experimental results for small thicknesses.

Analysis of body fluids for forensic purposes: from laboratory testing to non-destructive rapid confirmatory identification at a crime scene.

PubMed

Virkler, Kelly; Lednev, Igor K

2009-07-01

Body fluid traces recovered at crime scenes are among the most important types of evidence to forensic investigators. They contain valuable DNA evidence which can identify a suspect or victim as well as exonerate an innocent individual. The first step of identifying a particular body fluid is highly important since the nature of the fluid is itself very informative to the investigation, and the destructive nature of a screening test must be considered when only a small amount of material is available. The ability to characterize an unknown stain at the scene of the crime without having to wait for results from a laboratory is another very critical step in the development of forensic body fluid analysis. Driven by the importance for forensic applications, body fluid identification methods have been extensively developed in recent years. The systematic analysis of these new developments is vital for forensic investigators to be continuously educated on possible superior techniques. Significant advances in laser technology and the development of novel light detectors have dramatically improved spectroscopic methods for molecular characterization over the last decade. The application of this novel biospectroscopy for forensic purposes opens new and exciting opportunities for the development of on-field, non-destructive, confirmatory methods for body fluid identification at a crime scene. In addition, the biospectroscopy methods are universally applicable to all body fluids unlike the majority of current techniques which are valid for individual fluids only. This article analyzes the current methods being used to identify body fluid stains including blood, semen, saliva, vaginal fluid, urine, and sweat, and also focuses on new techniques that have been developed in the last 5-6 years. In addition, the potential of new biospectroscopic techniques based on Raman and fluorescence spectroscopy is evaluated for rapid, confirmatory, non-destructive identification of a body

Non-destructive imaging of fragments of historical beeswax seals using high-contrast X-ray micro-radiography and micro-tomography with large area photon-counting detector array.

PubMed

Karch, Jakub; Bartl, Benjamin; Dudak, Jan; Zemlicka, Jan; Krejci, Frantisek

2016-12-01

Historical beeswax seals are unique cultural heritage objects. Unfortunately, a number of historical sealing waxes show a porous structure with a strong tendency to stratification and embrittlement, which makes these objects extremely prone to mechanical damage. The understanding of beeswax degradation processes therefore plays an important role in the preservation and consequent treatment of these objects. Conventional methods applied for the investigation of beeswax materials (e.g. gas chromatography) are of a destructive nature or bring only limited information about the sample surface (microscopic techniques). Considering practical limitations of conventional methods and ethical difficulties connected with the sampling of the historical material, radiation imaging methods such as X-ray micro-tomography presents a promising non-destructive tool for the onward scientific research in this field. In this contribution, we present the application of high-contrast X-ray micro-radiography and micro-tomography for the investigation of beeswax seal fragments. The method is based on the application of the large area photon-counting detector recently developed at our institute. The detector combines the advantages of single-photon counting technology with a large field of view. The method, consequently, enables imaging of relatively large objects with high geometrical magnification. In the reconstructed micro-tomographies of investigated historical beeswax seals, we are able to reveal morphological structures such as stratification, micro-cavities and micro-fractures with spatial resolution down to 5μm non-destructively and with high imaging quality. The presented work therefore demonstrates that a combination of state-of-the-art hybrid pixel semiconductor detectors and currently available micro-focus x-ray sources makes it possible to apply X-ray micro-radiography and micro-tomography as a valuable non-destructive tool for volumetric beeswax seal morphological studies

Non-Destructive Classification Approaches for Equilbrated Ordinary Chondrites

NASA Technical Reports Server (NTRS)

Righter, K.; Harrington, R.; Schroeder, C.; Morris, R. V.

2013-01-01

Classification of meteorites is most effectively carried out by petrographic and mineralogic studies of thin sections, but a rapid and accurate classification technique for the many samples collected in dense collection areas (hot and cold deserts) is of great interest. Oil immersion techniques have been used to classify a large proportion of the US Antarctic meteorite collections since the mid-1980s [1]. This approach has allowed rapid characterization of thousands of samples over time, but nonetheless utilizes a piece of the sample that has been ground to grains or a powder. In order to compare a few non-destructive techniques with the standard approaches, we have characterized a group of chondrites from the Larkman Nunatak region using magnetic susceptibility and Moessbauer spectroscopy.

Non-destructive evaluation means and method of flaw reconstruction utilizing an ultrasonic multi-viewing transducer data acquistion system

DOEpatents

Thompson, Donald O.; Wormley, Samuel J.

1989-03-28

A multi-viewing ultrasound transducer acquisition system for non-destructive evaluation, flaw detection and flaw reconstruction in materials. A multiple transducer assembly includes a central transducer surrounded by a plurality of perimeter transducers, each perimeter transducer having an axis of transmission which can be angularly oriented with respect to the axis of transmission of the central transducer to intersect the axis of transmission of the central transducer. A control apparatus automatically and remotely positions the transducer assembly with respect to the material by a positioning apparatus and adjusts the pe GRANT REFERENCE This invention was conceived and reduced to practice at least in part under a grant from the Department of Energy under Contract No. W-7407-ENG-82.

Automatic Non-Destructive Growth Measurement of Leafy Vegetables Based on Kinect

PubMed Central

Hu, Yang; Wang, Le; Xiang, Lirong; Wu, Qian; Jiang, Huanyu

2018-01-01

Non-destructive plant growth measurement is essential for plant growth and health research. As a 3D sensor, Kinect v2 has huge potentials in agriculture applications, benefited from its low price and strong robustness. The paper proposes a Kinect-based automatic system for non-destructive growth measurement of leafy vegetables. The system used a turntable to acquire multi-view point clouds of the measured plant. Then a series of suitable algorithms were applied to obtain a fine 3D reconstruction for the plant, while measuring the key growth parameters including relative/absolute height, total/projected leaf area and volume. In experiment, 63 pots of lettuce in different growth stages were measured. The result shows that the Kinect-measured height and projected area have fine linear relationship with reference measurements. While the measured total area and volume both follow power law distributions with reference data. All these data have shown good fitting goodness (R2 = 0.9457–0.9914). In the study of biomass correlations, the Kinect-measured volume was found to have a good power law relationship (R2 = 0.9281) with fresh weight. In addition, the system practicality was validated by performance and robustness analysis. PMID:29518958

Evaluation of the MIT-Scan-T2 for non-destructive PCC pavement thickness determination.

DOT National Transportation Integrated Search

2008-07-01

The MIT-Scan-T2 device is marketed as a non-destructive way to determine pavement thickness on both : HMA and PCC pavements. PCC pavement thickness determination is an important incentivedisincentive : measurement for the Iowa DOT and contractors. Th...

Non-destructive examination of interfacial debonding using acoustic emission.

PubMed

Li, Haiyan; Li, Jianying; Yun, Xiaofei; Liu, Xiaozhou; Fok, Alex Siu-Lun

2011-10-01

This study aims to assess the viability of using the acoustic emission (AE) measurement technique to detect and monitor in situ the interfacial debonding in resin composite restorations due to build-up of shrinkage stresses during polymerization of the composite. The non-destructive testing technique that measures acoustic emission (AE) was used to detect and monitor the interfacial debonding in resin composite during curing of the composite. Four groups of specimens, n=4 each, were tested: (1) intact human molars with Class-I cavities restored with the composite Z100 (3M ESPE, USA); (2) intact human molars with Class-I cavities restored with the composite Filtek™ P90 (3M ESPE, USA); (3) ring samples prepared from the root of a single bovine tooth and 'restored' with Z100; (4) freestanding pea-size specimens of Z100 directly placed on the AE sensor. The restorations in Groups (1)-(3) were bonded to the tooth tissues with the adhesive Adper™ Scotchbond™ SE Self-Etch (3M ESPE, USA). The composites in all the specimens were cured with a blue light (3M ESPE, USA) for 40s. The AE signals were recorded continuously for 10 min from the start of curing. Non-destructive 3D imaging was performed using X-ray micro-computed tomography (micro-CT) to examine the bonding condition at the tooth-restoration interface. The development of AE events followed roughly that of the shrinkage stress, which was determined separately by the cantilever beam method. The number of AE events in the real human tooth samples was more than that in the ring samples, and no AE events were detected in the pea-size specimens placed directly on the AE sensor. The number of AE events recorded in the specimens restored using Z100 was more than that found in specimens restored with Filtek P90. The micro-CT imaging results showed clear interfacial debondings in the tooth specimens restored with Z100 after curing, but no clear debonding was found in the P90 specimens. The AE technique is an effective

Pre-processing of data coming from a laser-EMAT system for non-destructive testing of steel slabs.

PubMed

Sgarbi, Mirko; Colla, Valentina; Cateni, Sivia; Higson, Stuart

2012-01-01

Non destructive test systems are increasingly applied in the industrial context for their strong potentialities in improving and standardizing quality control. Especially in the intermediate manufacturing stages, early detection of defects on semi-finished products allow their direction towards later production processes according to their quality, with consequent considerable savings in time, energy, materials and work. However, the raw data coming from non destructive test systems are not always immediately suitable for sophisticated defect detection algorithms, due to noise and disturbances which are unavoidable, especially in harsh operating conditions, such as the ones which are typical of the steelmaking cycle. The paper describes some pre-processing operations which are required in order to exploit the data coming from a non destructive test system. Such a system is based on the joint exploitation of Laser and Electro-Magnetic Acoustic Transducer technologies and is applied to the detection of surface and sub-surface cracks in cold and hot steel slabs. Copyright © 2011 ISA. Published by Elsevier Ltd. All rights reserved.

Non-destructive testing (NDT) of a segmental concrete bridge scheduled for demolition, with a focus on condition assessment and corrosion detection of internal tendons : [summary].

DOT National Transportation Integrated Search

2017-06-01

In this project, Florida International University researchers used segments from a demolished concrete segmental bridge with internal tendons to study damage to post-stressed tendons and to test the effectiveness of various methods of non-destructive...

Non-destructive testing of ceramic materials using mid-infrared ultrashort-pulse laser

NASA Astrophysics Data System (ADS)

Sun, S. C.; Qi, Hong; An, X. Y.; Ren, Y. T.; Qiao, Y. B.; Ruan, Liming M.

2018-04-01

The non-destructive testing (NDT) of ceramic materials using mid-infrared ultrashort-pulse laser is investigated in this study. The discrete ordinate method is applied to solve the transient radiative transfer equation in 2D semitransparent medium and the emerging radiative intensity on boundary serves as input for the inverse analysis. The sequential quadratic programming algorithm is employed as the inverse technique to optimize objective function, in which the gradient of objective function with respect to reconstruction parameters is calculated using the adjoint model. Two reticulated porous ceramics including partially stabilized zirconia and oxide-bonded silicon carbide are tested. The retrieval results show that the main characteristics of defects such as optical properties, geometric shapes and positions can be accurately reconstructed by the present model. The proposed technique is effective and robust in NDT of ceramics even with measurement errors.

The RAMANITA © method for non-destructive and in situ semi-quantitative chemical analysis of mineral solid-solutions by multidimensional calibration of Raman wavenumber shifts

NASA Astrophysics Data System (ADS)

Smith, David C.

2005-08-01

The "RAMANITA ©" method, for semi-quantitative chemical analysis of mineral solid-solutions by multidimensional calibration of Raman wavenumber shifts and mathematical calculation by simultaneous equations, is published here in detail in English for the first time. It was conceived by the present writer 20 years ago for binary and ternary pyroxene and garnet systems. The mathematical description was set out in 1989, but in an abstract in an obscure French special publication. Detailed "step-by-step" calibration of two garnet ternaries, followed by their linking, by M. Pinet and D.C. Smith in the early 1990s provided a hexary garnet database. Much later, using this garnet database, which forms part of his personal database called RAMANITA ©, the present writer began to develop the method by improving the terminology, automating the calculations, discussing problems and experimenting with different real chemical problems in archaeometry. Although this RAMANITA © method has been very briefly mentioned in two recent books, the necessary full mathematical explanation is given only here. The method will find application in any study which requires obtaining a non-destructive semi-quantitative chemical analysis from mineral solid solutions that cannot be analysed by any destructive analytical method, in particular for archaeological, geological or extraterrestrial research projects, e.g. from gemstones or other crystalline artworks of the cultural heritage (especially by Mobile Raman Microscopy (MRM)) in situ in museums or at archaeological sites, including under water for subaquatic archaeometry; from scientifically precious mineral microinclusions (such as garnet or pyroxene within diamond); from minerals in rocks analysed in situ on planetary bodies by a rover (especially "at distance" by telescopy). Recently some other workers have begun deducing chemical compositions from Raman wavenumber shifts in multivariate chemical space, but the philosophical approach is

Non destructive testing of soft body armor

NASA Astrophysics Data System (ADS)

Bhise, Karan

Pristine bullet proof vests are extremely effective at halting pre-determined projectile threats and have saved over 3000 lives. However, the effectiveness of these vests to halt a bullet is seen to decrease over time.Owing to the importance of bullet proof vests over a period of time, tests to determine their effectiveness have been carried out on every batch of vests at the time of inception and at certain time intervals by shooting a bullet through them. A few vests from every batch are picked up and shot at to check for bullet penetration during this process while these results are extrapolated onto the other vests from the batch.One of the main issues with this method is the fact that testing a few jackets among a large set of jackets does not guarantee the safety of every jacket in the entire batch.Further the jackets that are shot-at have the possibility of undergoing substantial damage during the process thus compromising its safety rendering them unsafe for future use.As the vest penetration phenomenon is extremely complex too, there arose a need for a better testing procedure that could not only help ensure more safety, but also save time and money.The new testing procedure proposed a non-destructive evaluation of the jackets that would solve the issues previous faced in testing the vests. This would lead to the building of a portable set up which could be carried to any location to test jackets in a matter of minutes thus saving time and money.

Data transformation methods for multiplexed assays

DOEpatents

Tammero, Lance F. Bentley; Dzenitis, John M; Hindson, Benjamin J

2013-07-23

Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.

Research Performed within the Non-Destructive Evaluation Team at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Burns, Erin A.

2004-01-01

Non-destructive testing is essential in many fields of manufacturing and research in order to perform reliable examination of potentially damaged materials and parts without destroying the inherent structure of the materials. Thus, the Non-Destructive Evaluation (NDE) Team at NASA Glenn Research Center partakes in various projects to improve materials testing equipment as well as analyze materials, material defects, and material deficiencies. Due to the array of projects within the NDE Team at this time, five research aims were supplemental to some current projects. A literature survey of "DE and testing methodologies as related to rocks was performed. Also, Mars Expedition Rover technology was assessed to understand the requirements for instrumentation in harsh space environments (e.g. temperature). Potential instrumentation and technologies were also considered and documented. The literature survey provided background and potential sources for a proposal to acquire funding for ultrasonic instrumentation on board a future Mars expedition. The laboratory uses a Santec Systems AcousticScope AS200 acoustography system. Labview code was written within the current program in order to improve the current performance of the acoustography system. A sample of Reinforced Carbon/Carbon (RCC) material from the leading edge of the space shuttle underwent various non-destructive tests (guided wave scanning, thermography, computed tomography, real time x-ray, etc.) in order to characterize its structure and examine possible defects. Guided wave scan data of a ceramic matrix composite (CMC) panel was reanalyzed utilizing image correlations and signal processing variables. Additional guided wave scans and thermography were also performed on the CMC panel. These reevaluated data and images will be used in future presentations and publications. An additional axis for the guided wave scanner was designed, constructed, and implemented. This additional axis allowed incremental spacing

Non-destructive in situ mapping of macroholes, cracks and inhomogeneities of stalagmites in cave environments

NASA Astrophysics Data System (ADS)

Hegymegi, Erika; Gyöngy, Miklós; Bodolai, Tamás; Divós, Ferenc; Barta, Edit; Gribovszki, Katalin; Bokelmann, Götz; Hegymegi, Csaba; Lednická, Markéta; Kovács, Károly

2016-04-01

Intact and vulnerable, candle-stick type stalagmites can be used as prehistoric-earthquake indicators during seismic-hazard analysis of a given region, because they are old enough to survive several earthquakes. The continued intactness of the stalagmites indicates a lack of earthquakes that had the strength to destroy them. To make sure that the stalagmites are intact, we have to image their internal structure in order to estimate the steadiness more accurate and potential failure in the last few thousand years, during their evolution. These stalagmites play an important indicator role and carry fundamental information; however, legally they are strictly protected natural objects in Europe. Therefore it is impossible to examine them in the laboratory by conventional equipment such as computer tomography (CT) or X-ray, because this would require taking samples. With the presented non-destructive methods (ultrasound and acoustic tomography) we tried to detect macroholes, cracks and velocity anomalies inside the stalagmites on the mm scale in situ, in the cave. The acoustic tomography applied in the current work is an existing method in forest research. Forest researchers use it to non-destructively detect the size and location of decayed or hollow parts in the trunk and this technique is able to detect the velocity changing of wave propagation and anomalies in the stalagmites as well. The other method that we use is ultrasound imaging, which uses (and is able to calculate) the velocity of sound propagation. Here, the frequency used is much higher (typically 250 kHz to 5 MHz), which increases resolution but at the same time decreases penetration depth compared to acoustic tomography. In this latter work, through transmission and TOFD (time-of-flight-diffraction) ultrasound methods are using thickness-mode ultrasound transducers (Panametrics, Olympus). Such equipment is well-adapted to the cave environment and this is the first time that it has been used for these

Non-destructive evaluation of chlorophyll content in quinoa and amaranth leaves by simple and multiple regression analysis of RGB image components.

PubMed

Riccardi, M; Mele, G; Pulvento, C; Lavini, A; d'Andria, R; Jacobsen, S-E

2014-06-01

Leaf chlorophyll content provides valuable information about physiological status of plants; it is directly linked to photosynthetic potential and primary production. In vitro assessment by wet chemical extraction is the standard method for leaf chlorophyll determination. This measurement is expensive, laborious, and time consuming. Over the years alternative methods, rapid and non-destructive, have been explored. The aim of this work was to evaluate the applicability of a fast and non-invasive field method for estimation of chlorophyll content in quinoa and amaranth leaves based on RGB components analysis of digital images acquired with a standard SLR camera. Digital images of leaves from different genotypes of quinoa and amaranth were acquired directly in the field. Mean values of each RGB component were evaluated via image analysis software and correlated to leaf chlorophyll provided by standard laboratory procedure. Single and multiple regression models using RGB color components as independent variables have been tested and validated. The performance of the proposed method was compared to that of the widely used non-destructive SPAD method. Sensitivity of the best regression models for different genotypes of quinoa and amaranth was also checked. Color data acquisition of the leaves in the field with a digital camera was quick, more effective, and lower cost than SPAD. The proposed RGB models provided better correlation (highest R (2)) and prediction (lowest RMSEP) of the true value of foliar chlorophyll content and had a lower amount of noise in the whole range of chlorophyll studied compared with SPAD and other leaf image processing based models when applied to quinoa and amaranth.

High-resolution non-destructive three-dimensional imaging of integrated circuits

NASA Astrophysics Data System (ADS)

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H. R.; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-01

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography—a high-resolution coherent diffractive imaging technique—can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

High-resolution non-destructive three-dimensional imaging of integrated circuits.

PubMed

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H R; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-15

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography-a high-resolution coherent diffractive imaging technique-can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

A non-destructive method for quality control of the pellet distribution within a MUPS tablet by terahertz pulsed imaging.

PubMed

Novikova, Anna; Markl, Daniel; Zeitler, J Axel; Rades, Thomas; Leopold, Claudia S

2018-01-01

Terahertz pulsed imaging (TPI) was applied to analyse the inner structure of multiple unit pellet system (MUPS) tablets. MUPS tablets containing different amounts of theophylline pellets coated with Eudragit® NE 30 D and with microcrystalline cellulose (MCC) as cushioning agent were analysed. The tablets were imaged by TPI and the results were compared to X-ray microtomography. The terahertz pulse beam propagates through the tablets and is back-reflected at the interface between the MCC matrix and the coated pellets within the tablet causing a peak in the terahertz waveform. Cross-section images of the tablets were extracted at different depths and parallel to the tablet faces from 3D terahertz data to visualise the surface-near structure of the MUPS tablets. The images of the surface-near structure of the MUPS tablets were compared to X-ray microtomography images at the same depths. The surface-near structure could be clearly resolved by TPI at depths between 24 and 152μm below the tablet surface. An increasing amount of pellets within the MUPS tablets appears to slightly decrease the detectability of the pellets within the tablets by TPI. TPI was shown to be a non-destructive method for the detection of pellets within the tablets and could resolve structures thicker than 30μm. In conclusion, a proof-of-concept was provided for TPI as a method of quality control for MUPS tablets. Copyright © 2017 Elsevier B.V. All rights reserved.

Non-destructive terahertz imaging of illicit drugs using spectral fingerprints

NASA Astrophysics Data System (ADS)

Kawase, Kodo; Ogawa, Yuichi; Watanabe, Yuuki; Inoue, Hiroyuki

2003-10-01

The absence of non-destructive inspection techniques for illicit drugs hidden in mail envelopes has resulted in such drugs being smuggled across international borders freely. We have developed a novel basic technology for terahertz imaging, which allows detection and identification of drugs concealed in envelopes, by introducing the component spatial pattern analysis. The spatial distributions of the targets are obtained from terahertz multispectral transillumination images, using absorption spectra measured with a tunable terahertz-wave source. The samples we used were methamphetamine and MDMA, two of the most widely consumed illegal drugs in Japan, and aspirin as a reference.

Methods for threshold determination in multiplexed assays

DOEpatents

Tammero, Lance F. Bentley; Dzenitis, John M; Hindson, Benjamin J

2014-06-24

Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.

Non-equilibrium plasma reactors for organic solvent destruction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, C.L.; Beltran, M.R.; Kravets, Z.

1997-12-31

Two non-equilibrium plasma reactors were evaluated for their ability to destroy three widely used organic solvents, i.e., 2-butanone, toluene and ethyl acetate. The catalyzed plasma reactor (CPR) with 6 mm glass beads destroys 98% of 50 ppm toluene in air at 24 kV/cm and space velocities of 1,400 v/v/hr. Eighty-five percent of ethyl acetate and 2-butanone are destroyed under the same conditions. The tubular plasma reactor (TPR) has an efficiency of 10% to 20% lower than that of a CPR under the same conditions. The 1,400 v/v/hr in a CPR is equal to a residence time of 2.6 seconds inmore » a TPR. The operating temperatures, corona characteristics, as well as the kinetics of VOC destruction in both TPR and CPR were studied.« less

Non-destructive testing of satellite nozzles made of carbon fibre ceramic matrix composite, C/SiC

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rebelo Kornmeier, J.; Hofmann, M.; Schmidt, S.

Carbon fibre ceramic matrix composite materials, C/SiC, are excellent candidates as lightweight structural materials for high performance hot structures such as in aerospace applications. Satellite nozzles are manufactured from C/SiC, using, for instance, the Liquid Polymer Infiltration (LPI) process. In this article the applicability of different non-destructive analysis methods for the characterisation of C/SiC components will be discussed. By using synchrotron and neutron tomography it is possible to characterise the C/SiC material in each desired location or orientation. Synchrotron radiation using tomography on small samples with a resolution of 1.4 {mu}m, i.e. the fibre scale, was used to characterise threemore » dimensionally fibre orientation and integrity, matrix homogeneity and dimensions and distributions of micro pores. Neutron radiation tomography with a resolution of about 300 {mu}m was used to analyse the over-all C/SiC satellite nozzle component with respect to the fibre content. The special solder connection of a C/SiC satellite nozzle to a metallic ring was also successfully analysed by neutron tomography. In addition, the residual stress state of a temperature tested satellite nozzle was analysed non-destructively in depth by neutron diffraction. The results revealed almost zero stress for the principal directions, radial, axial and tangential, which can be considered to be the principal directions.« less

Measurement of Meteorite Density, Porosity and Magnetic Susceptibility: Fast, Non- destructive, Non-contaminating and Very Informative

NASA Astrophysics Data System (ADS)

Macke, R. J.; Britt, D. T.; Consolmagno, G. J.

2009-05-01

The development of the "glass bead" method [1] for measuring bulk density, coupled with other fast, non- destructive and non-contaminating methods for measuring grain density and magnetic susceptibility, has enabled broad surveys of large meteorite collections. We have employed these methods extensively on meteorites in numerous collections, including those at the Vatican, the American Museum of Natural History (New York), the National Museum of Natural History (Washington, DC), Texas Christian University, University of New Mexico, and Arizona State University. We present here a summary of some of the findings to date. Using the glass bead method, the meteorite is placed into a container which is then filled entirely with small (sub- millimeter) glass beads. The beads behave collectively as an Archimedean fluid, flowing around the sample to fill the empty space in the container. Through mass measurement, the volume displaced by the sample can be determined. Grain density is determined via helium ideal-gas pycnometry. Magnetic susceptibility is determined using a commercially available hand-held device [2]. Among notable findings to date, grain density and magnetic susceptibility together can distinguish H, L and LL ordinary chondrite falls into clearly distinct groupings [3]. On the other hand, enstatite chondrites of EH and EL subgroups are indistinguishable in these properties, indicating that EH and EL do not differ significantly in iron content [4]. Carbonaceous chondrites can have porosities that are significantly higher than ordinary chondrites and (especially for aqueously altered meteorites) lower density, though these also vary according to subgroups [5]. References: [1] Consolmagno and Britt, 1998. M&PS 33, 1231-1240. [2] Gattacceca et al., 2004. GJI 158, 42-49. [3] Consolmagno et al., 2006. M&PS 41, 331-342. [4] Macke et al., 2009. LPSC 40, 1598. [5] Consolmagno et al., 2008. MetSoc 71, 5038.

Direct, non-destructive, and rapid evaluation of developmental cotton fibers by ATR FT-IR spectroscopy

USDA-ARS?s Scientific Manuscript database

Chemical, compositional, and structural differences within the fibers at different growth stages have been investigated considerably through a number of methodologies. Due to its direct, non-destructive, and rapid attribute, this study reports the utilization of attenuated total reflection Fourier t...

The Effect of Localized Damage on the Electrical Conductivity of Bare Carbon Fiber Tow and its Use as a Non-Destructive Evaluation Tool for Composite Overwrapped Pressure Vessels

NASA Technical Reports Server (NTRS)

Wentzel, Daniel

2015-01-01

Composite materials are beneficial because of their high specific strength and low weight. Safety, Destructive testing and destructive testing, Non-Destructive Testing (NDT) and Non-Destructive Evaluation (NDE). Problem: Neither NDT nor NDE can provide sufficient data to determine life expectancy or quantify the damage state of a composite material.

Toward a simple, repeatable, non-destructive approach to measuring stable-isotope ratios of water within tree stems

NASA Astrophysics Data System (ADS)

Raulerson, S.; Volkmann, T.; Pangle, L. A.

2017-12-01

Traditional methodologies for measuring ratios of stable isotopes within the xylem water of trees involve destructive coring of the stem. A recent approach involves permanently installed probes within the stem, and an on-site assembly of pumps, switching valves, gas lines, and climate-controlled structure for field deployment of a laser spectrometer. The former method limits the possible temporal resolution of sampling, and sample size, while the latter may not be feasible for many research groups. We present results from initial laboratory efforts towards developing a non-destructive, temporally-resolved technique for measuring stable isotope ratios within the xylem flow of trees. Researchers have used direct liquid-vapor equilibration as a method to measure isotope ratios of the water in soil pores. Typically, this is done by placing soil samples in a fixed container, and allowing the liquid water within the soil to come into isotopic equilibrium with the headspace of the container. Water can also be removed via cryogenic distillation or azeotropic distillation, with the resulting liquid tested for isotope ratios. Alternatively, the isotope ratios of the water vapor can be directly measured using a laser-based water vapor isotope analyzer. Well-established fractionation factors and the isotope ratios in the vapor phase are then used to calculate the isotope ratios in the liquid phase. We propose a setup which would install a single, removable chamber onto a tree, where vapor samples could non-destructively and repeatedly be taken. These vapor samples will be injected into a laser-based isotope analyzer by a recirculating gas conveyance system. A major part of what is presented here is in the procedure of taking vapor samples at 100% relative humidity, appropriately diluting them with completely dry N2 calibration gas, and injecting them into the gas conveyance system without inducing fractionation in the process. This methodology will be helpful in making

Coded excitation for infrared non-destructive testing of carbon fiber reinforced plastics.

PubMed

Mulaveesala, Ravibabu; Venkata Ghali, Subbarao

2011-05-01

This paper proposes a Barker coded excitation for defect detection using infrared non-destructive testing. Capability of the proposed excitation scheme is highlighted with recently introduced correlation based post processing approach and compared with the existing phase based analysis by taking the signal to noise ratio into consideration. Applicability of the proposed scheme has been experimentally validated on a carbon fiber reinforced plastic specimen containing flat bottom holes located at different depths.

Non-destructive evaluation of water ingress in photovoltaic modules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bora, Mihail; Kotovsky, Jack

Systems and techniques for non-destructive evaluation of water ingress in photovoltaic modules include and/or are configured to illuminate a photovoltaic module comprising a photovoltaic cell and an encapsulant with at least one beam of light having a wavelength in a range from about 1400 nm to about 2700 nm; capture one or more images of the illuminated photovoltaic module, each image relating to a water content of the photovoltaic module; and determine a water content of the photovoltaic module based on the one or more images. Systems preferably include one or more of a light source, a moving mirror, amore » focusing lens, a beam splitter, a stationary mirror, an objective lens and an imaging module.« less

Spectral algorithm for non-destructive damage localisation: Application to an ancient masonry arch model

NASA Astrophysics Data System (ADS)

Masciotta, Maria-Giovanna; Ramos, Luís F.; Lourenço, Paulo B.; Vasta, Marcello

2017-02-01

Structural monitoring and vibration-based damage identification methods are fundamental tools for condition assessment and early-stage damage identification, especially when dealing with the conservation of historical constructions and the maintenance of strategic civil structures. However, although the substantial advances in the field, several issues must still be addressed to broaden the application range of such tools and to assert their reliability. This study deals with the experimental validation of a novel method for non-destructive damage identification purposes. This method is based on the use of spectral output signals and has been recently validated by the authors through a numerical simulation. After a brief insight into the basic principles of the proposed approach, the spectral-based technique is applied to identify the experimental damage induced on a masonry arch through statically increasing loading. Once the direct and cross spectral density functions of the nodal response processes are estimated, the system's output power spectrum matrix is built and decomposed in eigenvalues and eigenvectors. The present study points out how the extracted spectral eigenparameters contribute to the damage analysis allowing to detect the occurrence of damage and to locate the target points where the cracks appear during the experimental tests. The sensitivity of the spectral formulation to the level of noise in the modal data is investigated and discussed. As a final evaluation criterion, the results from the spectrum-driven method are compared with the ones obtained from existing non-model based damage identification methods.

Non-Destructive Evaluation for Corrosion Monitoring in Concrete: A Review and Capability of Acoustic Emission Technique

PubMed Central

Zaki, Ahmad; Chai, Hwa Kian; Aggelis, Dimitrios G.; Alver, Ninel

2015-01-01

Corrosion of reinforced concrete (RC) structures has been one of the major causes of structural failure. Early detection of the corrosion process could help limit the location and the extent of necessary repairs or replacement, as well as reduce the cost associated with rehabilitation work. Non-destructive testing (NDT) methods have been found to be useful for in-situ evaluation of steel corrosion in RC, where the effect of steel corrosion and the integrity of the concrete structure can be assessed effectively. A complementary study of NDT methods for the investigation of corrosion is presented here. In this paper, acoustic emission (AE) effectively detects the corrosion of concrete structures at an early stage. The capability of the AE technique to detect corrosion occurring in real-time makes it a strong candidate for serving as an efficient NDT method, giving it an advantage over other NDT methods. PMID:26251904

Non-Destructive Evaluation for Corrosion Monitoring in Concrete: A Review and Capability of Acoustic Emission Technique.

PubMed

Zaki, Ahmad; Chai, Hwa Kian; Aggelis, Dimitrios G; Alver, Ninel

2015-08-05

Corrosion of reinforced concrete (RC) structures has been one of the major causes of structural failure. Early detection of the corrosion process could help limit the location and the extent of necessary repairs or replacement, as well as reduce the cost associated with rehabilitation work. Non-destructive testing (NDT) methods have been found to be useful for in-situ evaluation of steel corrosion in RC, where the effect of steel corrosion and the integrity of the concrete structure can be assessed effectively. A complementary study of NDT methods for the investigation of corrosion is presented here. In this paper, acoustic emission (AE) effectively detects the corrosion of concrete structures at an early stage. The capability of the AE technique to detect corrosion occurring in real-time makes it a strong candidate for serving as an efficient NDT method, giving it an advantage over other NDT methods.

Assessment of recovery and recrystallisation behaviours of cold rolled IF steel through non-destructive electromagnetic characterisation

NASA Astrophysics Data System (ADS)

Roy, Rajat K.; Dutta, Siuli; Panda, Ashis K.; Rajinikanth, V.; Das, Swapan K.; Mitra, Amitava; Strangwood, M.; Davis, Claire L.

2018-07-01

The recovery and recrystallisation behaviours of cold rolled IF steel have been investigated by destructive (optical microscopy and hardness) and non-destructive electromagnetic sensor, (which allows direct measurement of strip samples with no surface preparation) techniques. The onset and completion of recrystallisation are clearly monitored through destructive techniques of optical microscopy and hardness measurements. The nucleation of new recrystallised grains is observed in the sample annealed at 600 °C/15 min, while completion of recrystallisation takes place at 700 °C/15 min. The destructive techniques are not very accurate in monitoring recovery, for example, changes in hardness of <20% are seen. In contrast, the magnetic properties of annealed steel show the onsets of both recovery and recrystallisation, with recovery accounting for ≈60% change in the coercivity value. Therefore, the measurement of magnetic softening through an electromagnetic sensor acts a crucial role for understanding recovery and recrystallisation behaviours of steels during industrial processing. The present investigation is aimed not only for controlling product quality but also saving characterisation time through off line monitoring during steel processing at industry.

Semiautomated Method for Microbiological Vitamin Assays

PubMed Central

Berg, T. M.; Behagel, H. A.

1972-01-01

A semiautomated method for microbiological vitamin assays is described, which includes separate automated systems for the preparation of the cultures and for the measurement of turbidity. In the dilution and dosage unit based on the continuous-flow principle, vitamin samples were diluted to two different dose levels at a rate of 40 per hr, mixed with the inoculated test broth, and dispensed into culture tubes. After incubation, racks with culture tubes were placed on the sampler of an automatic turbidimeter. This unit, based on the discrete-sample system, measured the turbidity and printed the extinction values at a rate of 300 per hr. Calculations were computerized and the results, including statistical data, are presented in an easily readable form. The automated method is in routine use for the assays of thiamine, riboflavine, pyridoxine, cyanocobalamin, calcium pantothenate, nicotinic acid, pantothenol, and folic acid. Identical vitamin solutions assayed on different days gave variation coefficients for the various vitamin assays of less than 10%. Images PMID:4553802

Non-destructive investigation of a time capsule using neutron radiography and X-ray fluorescence

NASA Astrophysics Data System (ADS)

MacDonald, B. L.; Vanderstelt, J.; O'Meara, J.; McNeill, F. E.

2016-01-01

Non-destructive analytical techniques are becoming increasingly important for the study of objects of cultural heritage interest. This study applied two techniques: X-ray fluorescence and neutron radiography, for the investigation of a capped, tubular metal object recovered from an urban construction site in Gore Park, Hamilton, Canada. The site is an urban park containing a World War I commemorative monument that underwent renovation and relocation. Historical documentation suggested that the object buried underneath the monument was a time capsule containing a paper document listing the names of 1800 Canadians who died during WWI. The purpose of this study was to assess the condition of the object, and to verify if it was what the historical records purported. XRF analysis was used to characterize the elemental composition of the metal artifact, while neutron radiography revealed that its contents were congruent with historical records and remained intact after being interred for 91 years. Results of this study demonstrate the value of non-destructive techniques for the analysis and preservation of cultural heritage.

Onsite Gaseous Centrifuge Enrichment Plant UF6 Cylinder Destructive Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anheier, Norman C.; Cannon, Bret D.; Qiao, Hong

2012-07-17

The IAEA safeguards approach for gaseous centrifuge enrichment plants (GCEPs) includes measurements of gross, partial, and bias defects in a statistical sampling plan. These safeguard methods consist principally of mass and enrichment nondestructive assay (NDA) verification. Destructive assay (DA) samples are collected from a limited number of cylinders for high precision offsite mass spectrometer analysis. DA is typically used to quantify bias defects in the GCEP material balance. Under current safeguards measures, the operator collects a DA sample from a sample tap following homogenization. The sample is collected in a small UF6 sample bottle, then sealed and shipped under IAEAmore » chain of custody to an offsite analytical laboratory. Current practice is expensive and resource intensive. We propose a new and novel approach for performing onsite gaseous UF6 DA analysis that provides rapid and accurate assessment of enrichment bias defects. DA samples are collected using a custom sampling device attached to a conventional sample tap. A few micrograms of gaseous UF6 is chemically adsorbed onto a sampling coupon in a matter of minutes. The collected DA sample is then analyzed onsite using Laser Ablation Absorption Ratio Spectrometry-Destructive Assay (LAARS-DA). DA results are determined in a matter of minutes at sufficient accuracy to support reliable bias defect conclusions, while greatly reducing DA sample volume, analysis time, and cost.« less

Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants

PubMed Central

Nigmatullina, Liliia R.; Sharipova, Margarita R.; Shakirov, Eugene V.

2016-01-01

The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana and present evidence that this approach can be used successfully to efficiently and accurately measure telomere length in plants. Specifically, hybridization temperature of 42 °C instead of the typical 55 °C appears to generate stronger signals. In addition, DIG incorporation at 5′-end instead of 3′-end of the labeled oligonucleotide greatly enhances signal. We conclude that non-radioactive TRF assays can be as efficient as radioactive methods in detecting and measuring telomere length in plants, making this assay suitable for medical and research laboratories unable to utilize radioactivity due to hazardous waste disposal and safety concerns. PMID:28133587

SAFT-assisted sound beam focusing using phased arrays (PA-SAFT) for non-destructive evaluation

NASA Astrophysics Data System (ADS)

Nanekar, Paritosh; Kumar, Anish; Jayakumar, T.

2015-04-01

Focusing of sound has always been a subject of interest in ultrasonic non-destructive evaluation. An integrated approach to sound beam focusing using phased array and synthetic aperture focusing technique (PA-SAFT) has been developed in the authors' laboratory. The approach involves SAFT processing on ultrasonic B-scan image collected by a linear array transducer using a divergent sound beam. The objective is to achieve sound beam focusing using fewer elements than the ones required using conventional phased array. The effectiveness of the approach is demonstrated on aluminium blocks with artificial flaws and steel plate samples with embedded volumetric weld flaws, such as slag and clustered porosities. The results obtained by the PA-SAFT approach are found to be comparable to those obtained by conventional phased array and full matrix capture - total focusing method approaches.

Non-destructive testing of full-length bonded rock bolts based on HHT signal analysis

NASA Astrophysics Data System (ADS)

Shi, Z. M.; Liu, L.; Peng, M.; Liu, C. C.; Tao, F. J.; Liu, C. S.

2018-04-01

Full-length bonded rock bolts are commonly used in mining, tunneling and slope engineering because of their simple design and resistance to corrosion. However, the length of a rock bolt and grouting quality do not often meet the required design standards in practice because of the concealment and complexity of bolt construction. Non-destructive testing is preferred when testing a rock bolt's quality because of the convenience, low cost and wide detection range. In this paper, a signal analysis method for the non-destructive sound wave testing of full-length bonded rock bolts is presented, which is based on the Hilbert-Huang transform (HHT). First, we introduce the HHT analysis method to calculate the bolt length and identify defect locations based on sound wave reflection test signals, which includes decomposing the test signal via empirical mode decomposition (EMD), selecting the intrinsic mode functions (IMF) using the Pearson Correlation Index (PCI) and calculating the instantaneous phase and frequency via the Hilbert transform (HT). Second, six model tests are conducted using different grouting defects and bolt protruding lengths to verify the effectiveness of the HHT analysis method. Lastly, the influence of the bolt protruding length on the test signal, identification of multiple reflections from defects, bolt end and protruding end, and mode mixing from EMD are discussed. The HHT analysis method can identify the bolt length and grouting defect locations from signals that contain noise at multiple reflected interfaces. The reflection from the long protruding end creates an irregular test signal with many frequency peaks on the spectrum. The reflections from defects barely change the original signal because they are low energy, which cannot be adequately resolved using existing methods. The HHT analysis method can identify reflections from the long protruding end of the bolt and multiple reflections from grouting defects based on mutations in the instantaneous

Test plan for Geo-Cleanse{reg_sign} demonstration (in situ destruction of dense non-aqueous phase liquid (DNAPL))

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jerome, K.M.; Looney, B.B.; Accorsi, F.

1996-09-01

Soils and groundwater beneath an abandoned process sewer line in the A/M Area of the Savannah River Site (SRS) contain elevated levels of volatile organic compounds, specifically trichloroethylene (TCE) and tetrachloroethylene (PCE), two common chlorinated solvents. These compounds have low aqueous solubilities, thus when released to the subsurface in sufficient quantity, tend to exist as immiscible fluids or nonaqueous phase liquids (NAPLs). Because chlorinated solvents are also denser than water, they are referred to by the acronym DNAPLs, or dense non-aqueous phase liquids. Technologies targeted at the efficient characterization or removal of DNAPL are not currently proven. For example, mostmore » DNAPL studies rely on traditional soil and water sampling and the fortuitous observation of immiscible solvent. Once DNAPL is identified, soil excavation (which is only applicable to small contained spill sites) is the only proven cleanup method. New cleanup approaches based on destruction of DNAPL either in situ or ex situ have been proposed and tested at the pilot scale. The proposed demonstration, as described in this report will evaluate the applicability to DNAPL plumes of a technology proven for in situ destruction of light non-aqueous phase liquids (LNAPLs) such as oils.« less

Case study of a non-destructive treatment method for the remediation of military structures containing polychlorinated biphenyl contaminated paint.

PubMed

Saitta, Erin K H; Gittings, Michael J; Novaes-Card, Simone; Quinn, Jacqueline; Clausen, Christian; O'Hara, Suzanne; Yestrebsky, Cherie L

2015-08-01

Restricted by federal regulations and limited remediation options, buildings contaminated with paint laden with polychlorinated biphenyls (PCBs) have high costs associated with the disposal of hazardous materials. As opposed to current remediation methods which are often destructive and a risk to the surrounding environment, this study suggests a non-metal treatment system (NMTS) and a bimetallic treatment system (BTS) as versatile remediation options for painted industrial structures including concrete buildings, and metal machine parts. In this field study, four areas of a discontinued Department of Defense site were treated and monitored over 3 weeks. PCB levels in paint and treatment system samples were analyzed through gas chromatography/electron capture detection (GC-ECD). PCB concentrations were reduced by 95 percent on painted concrete and by 60-97 percent on painted metal with the majority of the PCB removal occurring within the first week of application. Post treatment laboratory studies including the utilization of an activated metal treatment system (AMTS) further degraded PCBs in BTS and NMTS by up to 82 percent and 99 percent, respectively, indicating that a two-step remediation option is viable. These findings demonstrate that the NMTS and BTS can be an effective, nondestructive, remediation process for large painted structures, allowing for the reuse or sale of remediated materials that otherwise may have been disposed. Copyright © 2015 Elsevier Ltd. All rights reserved.

A Monte Carlo approach applied to ultrasonic non-destructive testing

NASA Astrophysics Data System (ADS)

Mosca, I.; Bilgili, F.; Meier, T. M.; Sigloch, K.

2011-12-01

Non-destructive testing based on ultrasound allows us to detect, characterize and size discrete flaws in geotechnical and engineering structures and materials. This information is needed to determine whether such flaws can be tolerated in future service. In typical ultrasonic experiments, only the first-arriving P-wave is interpreted, and the remainder of the recorded waveform is neglected. Our work aims at understanding surface waves, which are strong signals in the later wave train, with the ultimate goal of full waveform tomography. At present, even the structural estimation of layered media is still challenging because material properties of the samples can vary widely, and good initial models for inversion do not often exist. The aim of the present study is to analyze ultrasonic waveforms measured at the surface of Plexiglas and rock samples, and to define the behaviour of surface waves in structures of increasing complexity. The tremendous potential of ultrasonic surface waves becomes an advantage only if numerical forward modelling tools are available to describe the waveforms accurately. We compute synthetic full seismograms as well as group and phase velocities for the data. We invert them for the elastic properties of the sample via a global search of the parameter space, using the Neighbourhood Algorithm. Such a Monte Carlo approach allows us to perform a complete uncertainty and resolution analysis, but the computational cost is high and increases quickly with the number of model parameters. Therefore it is practical only for defining the seismic properties of media with a limited number of degrees of freedom, such as layered structures. We have applied this approach to both synthetic layered structures and real samples. The former contributed to benchmark the propagation of ultrasonic surface waves in typical materials tested with a non-destructive technique (e.g., marble, unweathered and weathered concrete and natural stone).

Non-destructive spatial characterization of buried interfaces in multilayer stacks via two color picosecond acoustics

NASA Astrophysics Data System (ADS)

Faria, Jorge C. D.; Garnier, Philippe; Devos, Arnaud

2017-12-01

We demonstrate the ability to construct wide-area spatial mappings of buried interfaces in thin film stacks in a non-destructive manner using two color picosecond acoustics. Along with the extraction of layer thicknesses and sound velocities from acoustic signals, the morphological information presented is a powerful demonstration of phonon imaging as a metrological tool. For a series of heterogeneous (polymer, metal, and semiconductor) thin film stacks that have been treated with a chemical procedure known to alter layer properties, the spatial mappings reveal changes to interior thicknesses and chemically modified surface features without the need to remove uppermost layers. These results compare well to atomic force microscopy scans showing that the technique provides a significant advantage to current characterization methods for industrially important device stacks.

Resonant ultrasound spectroscopy and non-destructive testing

NASA Astrophysics Data System (ADS)

Migliori, A.; Darling, T. W.

The use of mechanical resonances to test properties of materials is perhaps older than the industrial revolution. Early documented cases of British railroad engineers tapping the wheels of a train and using the sound to detect cracks perhaps mark the first real use of resonances to test the integrity of high-performance alloys. Attempts were made in the following years to understand the resonances of solids mathematically, based on the shape and composition. But Nobel Laureate Lord Rayleigh best summarized the state of affairs in 1894, stating 'the problem has, for the most part, resisted attack'. More recently, modern computers and electronics have enabled Anderson and co-workers with their work on minerals, and our work at Los Alamos on new materials and manufactured components to advance the use of resonances to a precision non-destructive testing tool that makes anisotropic modulus measurements, defect detection and geometry error detection routine. The result is that resonances can achieve the highest absolute accuracy for any dynamic modulus measurement technique, can be used on the smallest samples, and can also enable detection of errors in certain classes of precision manufactured components faster and more accurately than any other technique.

Infrared non-destructive evaluation method and apparatus

DOEpatents

Baleine, Erwan; Erwan, James F; Lee, Ching-Pang; Stinelli, Stephanie

2014-10-21

A method of nondestructive evaluation and related system. The method includes arranging a test piece (14) having an internal passage (18) and an external surface (15) and a thermal calibrator (12) within a field of view (42) of an infrared sensor (44); generating a flow (16) of fluid characterized by a fluid temperature; exposing the test piece internal passage (18) and the thermal calibrator (12) to fluid from the flow (16); capturing infrared emission information of the test piece external surface (15) and of the thermal calibrator (12) simultaneously using the infrared sensor (44), wherein the test piece infrared emission information includes emission intensity information, and wherein the thermal calibrator infrared emission information includes a reference emission intensity associated with the fluid temperature; and normalizing the test piece emission intensity information against the reference emission intensity.

A high-throughput technique for determining grain boundary character non-destructively in microstructures with through-thickness grains

DOE PAGES

Seita, Matteo; Volpi, Marco; Patala, Srikanth; ...

2016-06-24

Grain boundaries (GBs) govern many properties of polycrystalline materials. However, because of their structural variability, our knowledge of GB constitutive relations is still very limited. We present a novel method to characterise the complete crystallography of individual GBs non-destructively, with high-throughput, and using commercially available tools. This method combines electron diffraction, optical reflectance and numerical image analysis to determine all five crystallographic parameters of numerous GBs in samples with through-thickness grains. We demonstrate the technique by measuring the crystallographic character of about 1,000 individual GBs in aluminum in a single run. Our method enables cost- and time-effective assembly of crystallography–propertymore » databases for thousands of individual GBs. Furthermore, such databases are essential for identifying GB constitutive relations and for predicting GB-related behaviours of polycrystalline solids.« less

A simple, sensitive and non-destructive technique for characterizing bovine dental enamel erosion: attenuated total reflection Fourier transform infrared spectroscopy

PubMed Central

Kim, In-Hye; Son, Jun Sik; Min, Bong Ki; Kim, Young Kyoung; Kim, Kyo-Han; Kwon, Tae-Yub

2016-01-01

Although many techniques are available to assess enamel erosion in vitro, a simple, non-destructive method with sufficient sensitivity for quantifying dental erosion is required. This study characterized the bovine dental enamel erosion induced by various acidic beverages in vitro using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Deionized water (control) and 10 acidic beverages were selected to study erosion, and the pH and neutralizable acidity were measured. Bovine anterior teeth (110) were polished with up to 1 200-grit silicon carbide paper to produce flat enamel surfaces, which were then immersed in 20 mL of the beverages for 30 min at 37 °C. The degree of erosion was evaluated using ATR-FTIR spectroscopy and Vickers' microhardness measurements. The spectra obtained were interpreted in two ways that focused on the ν1, ν3 phosphate contour: the ratio of the height amplitude of ν3 PO4 to that of ν1 PO4 (Method 1) and the shift of the ν3 PO4 peak to a higher wavenumber (Method 2). The percentage changes in microhardness after the erosion treatments were primarily affected by the pH of the immersion media. Regression analyses revealed highly significant correlations between the surface hardness change and the degree of erosion, as detected by ATR-FTIR spectroscopy (P<0.001). Method 1 was the most sensitive to these changes, followed by surface hardness change measurements and Method 2. This study suggests that ATR-FTIR spectroscopy is potentially advantageous over the microhardness test as a simple, non-destructive, sensitive technique for the quantification of enamel erosion. PMID:27025266

A simple, sensitive and non-destructive technique for characterizing bovine dental enamel erosion: attenuated total reflection Fourier transform infrared spectroscopy.

PubMed

Kim, In-Hye; Son, Jun Sik; Min, Bong Ki; Kim, Young Kyoung; Kim, Kyo-Han; Kwon, Tae-Yub

2016-03-30

Although many techniques are available to assess enamel erosion in vitro, a simple, non-destructive method with sufficient sensitivity for quantifying dental erosion is required. This study characterized the bovine dental enamel erosion induced by various acidic beverages in vitro using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Deionized water (control) and 10 acidic beverages were selected to study erosion, and the pH and neutralizable acidity were measured. Bovine anterior teeth (110) were polished with up to 1 200-grit silicon carbide paper to produce flat enamel surfaces, which were then immersed in 20 mL of the beverages for 30 min at 37 °C. The degree of erosion was evaluated using ATR-FTIR spectroscopy and Vickers' microhardness measurements. The spectra obtained were interpreted in two ways that focused on the ν1, ν3 phosphate contour: the ratio of the height amplitude of ν3 PO4 to that of ν1 PO4 (Method 1) and the shift of the ν3 PO4 peak to a higher wavenumber (Method 2). The percentage changes in microhardness after the erosion treatments were primarily affected by the pH of the immersion media. Regression analyses revealed highly significant correlations between the surface hardness change and the degree of erosion, as detected by ATR-FTIR spectroscopy (P<0.001). Method 1 was the most sensitive to these changes, followed by surface hardness change measurements and Method 2. This study suggests that ATR-FTIR spectroscopy is potentially advantageous over the microhardness test as a simple, non-destructive, sensitive technique for the quantification of enamel erosion.

Development of fluorescent methods for DNA methyltransferase assay

NASA Astrophysics Data System (ADS)

Li, Yueying; Zou, Xiaoran; Ma, Fei; Tang, Bo; Zhang, Chun-yang

2017-03-01

DNA methylation modified by DNA methyltransferase (MTase) plays an important role in regulating gene transcription, cell growth and proliferation. The aberrant DNA MTase activity may lead to a variety of human diseases including cancers. Therefore, accurate and sensitive detection of DNA MTase activity is crucial to biomedical research, clinical diagnostics and therapy. However, conventional DNA MTase assays often suffer from labor-intensive operations and time-consuming procedures. Alternatively, fluorescent methods have significant advantages of simplicity and high sensitivity, and have been widely applied for DNA MTase assay. In this review, we summarize the recent advances in the development of fluorescent methods for DNA MTase assay. These emerging methods include amplification-free and the amplification-assisted assays. Moreover, we discuss the challenges and future directions of this area.

A robust approach to optimal matched filter design in ultrasonic non-destructive evaluation (NDE)

NASA Astrophysics Data System (ADS)

Li, Minghui; Hayward, Gordon

2017-02-01

The matched filter was demonstrated to be a powerful yet efficient technique to enhance defect detection and imaging in ultrasonic non-destructive evaluation (NDE) of coarse grain materials, provided that the filter was properly designed and optimized. In the literature, in order to accurately approximate the defect echoes, the design utilized the real excitation signals, which made it time consuming and less straightforward to implement in practice. In this paper, we present a more robust and flexible approach to optimal matched filter design using the simulated excitation signals, and the control parameters are chosen and optimized based on the real scenario of array transducer, transmitter-receiver system response, and the test sample, as a result, the filter response is optimized and depends on the material characteristics. Experiments on industrial samples are conducted and the results confirm the great benefits of the method.

Non-destructive evaluation of containment walls in nuclear power plants

NASA Astrophysics Data System (ADS)

Garnier, V.; Payan, C.; Lott, M.; Ranaivomanana, N.; Balayssac, J. P.; Verdier, J.; Larose, E.; Zhang, Y.; Saliba, J.; Boniface, A.; Sbartai, Z. M.; Piwakowski, B.; Ciccarone, C.; Hafid, H.; Henault, J. M.; Buffet, F. Ouvrier

2017-02-01

Two functions are regularly tested on containment walls in order to anticipate a possible accident. The first is mechanical to resist a possible internal over-pressure and the second is to prevent leakage. The AAPR reference accident is the rupture of a pipe in the primary circuit of a nuclear plant. In this case, the pressure and temperature can reach 5 bar and 180°C in 20 seconds. The national project `Non-destructive testing of the containment structures of nuclear plants' aims at studying the non-destructive techniques capable to evaluate the concrete properties and its damaging and cracks. This 4-year-project is segmented into two parts. The first consists in developing and selecting the most relevant NDEs in the laboratory to reach these goals. These evaluations are developed in conditions representing the real conditions of the stresses generated during ten-yearly visits of the plants or those related to an accident. The second part consists in applying the selected techniques to two containment structures under pressure. The first structure is proposed by ONERA and the second is a mockup of a containment wall on a 1/3 scale made by EDF within the VeRCoRs project. Communication is focused on the part of the project that concerns the damage and crack process characterization by means of NDT. The tests are done in 3 or 4 points bending in order to study the cracks' generation, their propagation, as well as their opening and closing. The main ultrasonic techniques developed concern linear or non-linear acoustic: acoustic emission [1], Locadiff [2], energy diffusion, surface wave's velocity and attenuation, DAET [3]. The recorded data contribute to providing the mapping of the investigated parameters, either in volume, in surface or globally. Digital image correlation is an important additional asset to validate the coherence of the data. The spatial normalization of the data in the specimen space allows proposing algorithms on the combination of the

Non-destructive and rapid prediction of moisture content in red pepper (Capsicum annuum L.) powder using near-infrared spectroscopy and a partial least squares regression model

USDA-ARS?s Scientific Manuscript database

Purpose: The aim of this study was to develop a technique for the non-destructive and rapid prediction of the moisture content in red pepper powder using near-infrared (NIR) spectroscopy and a partial least squares regression (PLSR) model. Methods: Three red pepper powder products were separated in...

Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods.

PubMed

Li, Yun-He; Romeis, Jörg; Wu, Kong-Ming; Peng, Yu-Fa

2014-04-01

In assessing an insect-resistant genetically engineered (IRGE) crop before its commercialization, researchers normally use so-called "Tier-1 assays" as the initial step to determine the effects of the crop on non-target organisms. In these tests, the insecticidal proteins (IPs) produced by the IRGEs are added to the diets of test organisms in the laboratory. Test organisms in such assays can be directly exposed to much higher concentrations of the test IPs than they would encounter in the field. The results of Tier-1 assays are thus more conservative than those generated in studies in which the organisms are exposed to the IPs by feeding on IRGE plant tissue or in the case of predators or parasites, by feeding on invertebrate prey or hosts that have fed on IRGE plant tissue. In this report, we consider three important factors that must be considered in Tier-1 assays: (i) methods for delivery of the IP to the test organisms; (ii) the need for and selection of compounds used as positive controls; and (iii) methods for monitoring the concentration, stability and bioactivity of the IP during the assay. We also analyze the existing data from Tier-1 assays regarding the toxicity of Bt Cry proteins to non-target arthropod species. The data indicate that the widely used Bt proteins have no direct toxicity to non-target organisms. © 2013 Institute of Zoology, Chinese Academy of Sciences.

The applicability of a material-treatment laser pulse in non-destructive evaluations.

PubMed

Hrovatin, R; Petkovsek, R; Diaci, J; Mozina, J

2006-12-22

A practical optodynamic study was performed to determine the usability of different lengths of laser pulses for the generation of ultrasonic transients in a solid material. The aim of the study was to evaluate the possibility of a dual use for a laser pulse-for laser material processing, on the one hand, and for the ultrasonic wave generation on the other-with both processes being combined on the same production line. The propagation of the laser-generated ultrasonic waves is evaluated by detecting and measuring with a PID-controlled stabilized interferometer. Thus, both systems provided the basic tools, the generation and detection of ultrasonic waves, for an ultrasonic, laser-based, non-destructive material evaluation. The ultrasonic transients generated by 'classical' nanosecond laser pulses were compared with the transients generated by industrial laser pulses with a duration of a few tenths of a microsecond. The experimental results are compared with the results of a time-of-flight analysis that also involved part of a mode-conversion analysis for both regimes in a layered material structure. The differences between the two waveforms were assessed in terms of their visibility, wavelength and resolution. The limit values were calculated and estimated for the laser-pulse parameters, when such pulses are intended for use in an ultrasonic, laser-based, non-destructive evaluation. The possibility of using an industrial marking laser for laser ultrasound generation is thus demonstrated.

Non-destructive Ripeness Sensing by Using Proton NMR [Nuclear Magnetic Resonance

DOE R&D Accomplishments Database

Cho, Seong In; Krutz, G. W.; Stroshine, R. L.; Bellon, V.

1990-01-01

More than 80 kinds of fruits and vegetables are available in the United States. But only about 6 of them have their quality standards (Dull, 1986). In the 1990 Fresh Trends survey (Zind, 1990), consumers were asked to rate 16 characteristics important to their decision to purchase fresh produce. The four top ranking factors were ripeness/freshness, taste/flavor, appearance/condition and nutritional value. Of these surveyed, 96% rated ripeness/freshness as extremely important or very important. Therefore, the development of reliable grading or sorting techniques for fresh commodities is essential. Determination of fruit quality often involves cutting and tasting. Non-destructive quality control in fruit and vegetables is a goal of growers and distributors, as well as the food processing industry. Many nondestructive techniques have been evaluated including soft x-ray, optical transmission, near infrared radiation, and machine vision. However, there are few reports of successful non-destructive measurement of sugar content directly in fruit. Higher quality fruit could be harvested and available to consumers if a nondestructive sensor that detects ripeness level directly by measuring sugar content were available. Using proton Nuclear Magnetic Resonance (NMR) principle is the possibility. A nondestructive ripeness (or sweetness) sensor for fruit quality control can be developed with the proton NMR principle (Cho, 1989). Several feasibility studies were necessary for the ripeness sensor development. Main objectives in this paper was to investigate the feasibilities (1) to detect ripeness (or sweetness level) of raw fruit tissue with an high resolution proton NMR spectroscopy (200 MHz) and (2) to measure sugar content of intact fruit with a low resolution proton NMR spectroscopy (10 MHz).

A new fiber-optic non-contact compact laser-ultrasound scanner for fast non-destructive testing and evaluation of aircraft composites

PubMed Central

Pelivanov, Ivan; Buma, Takashi; Xia, Jinjun; Wei, Chen-Wei; O'Donnell, Matthew

2014-01-01

Laser ultrasonic (LU) inspection represents an attractive, non-contact method to evaluate composite materials. Current non-contact systems, however, have relatively low sensitivity compared to contact piezoelectric detection. They are also difficult to adjust, very expensive, and strongly influenced by environmental noise. Here, we demonstrate that most of these drawbacks can be eliminated by combining a new generation of compact, inexpensive fiber lasers with new developments in fiber telecommunication optics and an optimally designed balanced probe scheme. In particular, a new type of a balanced fiber-optic Sagnac interferometer is presented as part of an all-optical LU pump-probe system for non-destructive testing and evaluation of aircraft composites. The performance of the LU system is demonstrated on a composite sample with known defects. Wide-band ultrasound probe signals are generated directly at the sample surface with a pulsed fiber laser delivering nanosecond laser pulses at a repetition rate up to 76 kHz rate with a pulse energy of 0.6 mJ. A balanced fiber-optic Sagnac interferometer is employed to detect pressure signals at the same point on the composite surface. A- and B-scans obtained with the Sagnac interferometer are compared to those made with a contact wide-band polyvinylidene fluoride transducer. PMID:24737921

Additive Manufacturing (AM) Activities and Non-Destructive Evaluation (NDE) at GSFC

NASA Technical Reports Server (NTRS)

Jones, Justin S.

2017-01-01

NASA personnel will be meeting with a delegation from the Japan Aerospace Exploration Agency (JAXA) Office of Safety and Mission Assurance (OSMA) at Langley Research Center on 2217 through 3217. The purpose of the meeting is a technical interchange between NASA and JAXA to discuss Non-Destructive Evaluation (NDE) of Additive Manufacturing (AM) parts and the HALT process (relates to accelerated life testing). The visitors will be a small group of Japanese citizens. Goddard Space Flight Center (GSFC) has been asked to participate in the meeting, either in person or via teleconference. This presentation covers NDE efforts at GSFC and provides a cursory overview of AM and lab capabilities.

Non-destructive elemental analysis of a carbonaceous chondrite with direct current Muon beam at MuSIC.

PubMed

Terada, K; Sato, A; Ninomiya, K; Kawashima, Y; Shimomura, K; Yoshida, G; Kawai, Y; Osawa, T; Tachibana, S

2017-11-13

Electron- or X-ray-induced characteristic X-ray analysis has been widely used to determine chemical compositions of materials in vast research fields. In recent years, analysis of characteristic X-rays from muonic atoms, in which a muon is captured, has attracted attention because both a muon beam and a muon-induced characteristic X-ray have high transmission abilities. Here we report the first non-destructive elemental analysis of a carbonaceous chondrite using one of the world-leading intense direct current muon beam source (MuSIC; MUon Science Innovative Channel). We successfully detected characteristic muonic X-rays of Mg, Si, Fe, O, S and C from Jbilet Winselwan CM chondrite, of which carbon content is about 2 wt%, and the obtained elemental abundance pattern was consistent with that of CM chondrites. Because of its high sensitivity to carbon, non-destructive elemental analysis with a muon beam can be a novel powerful tool to characterize future retuned samples from carbonaceous asteroids.

Non-destructive evaluation of bacteria-infected watermelon seeds using visible/near-infrared hyperspectral imaging.

PubMed

Lee, Hoonsoo; Kim, Moon S; Song, Yu-Rim; Oh, Chang-Sik; Lim, Hyoun-Sub; Lee, Wang-Hee; Kang, Jum-Soon; Cho, Byoung-Kwan

2017-03-01

There is a need to minimize economic damage by sorting infected seeds from healthy seeds before seeding. However, current methods of detecting infected seeds, such as seedling grow-out, enzyme-linked immunosorbent assays, the polymerase chain reaction (PCR) and the real-time PCR have a critical drawbacks in that they are time-consuming, labor-intensive and destructive procedures. The present study aimed to evaluate the potential of visible/near-infrared (Vis/NIR) hyperspectral imaging system for detecting bacteria-infected watermelon seeds. A hyperspectral Vis/NIR reflectance imaging system (spectral region of 400-1000 nm) was constructed to obtain hyperspectral reflectance images for 336 bacteria-infected watermelon seeds, which were then subjected to partial least square discriminant analysis (PLS-DA) and a least-squares support vector machine (LS-SVM) to classify bacteria-infected watermelon seeds from healthy watermelon seeds. The developed system detected bacteria-infected watermelon seeds with an accuracy > 90% (PLS-DA: 91.7%, LS-SVM: 90.5%), suggesting that the Vis/NIR hyperspectral imaging system is effective for quarantining bacteria-infected watermelon seeds. The results of the present study show that it is possible to use the Vis/NIR hyperspectral imaging system for detecting bacteria-infected watermelon seeds. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Application of Near Infrared Reflectance Spectroscopy for Rapid and Non-Destructive Discrimination of Hulled Barley, Naked Barley, and Wheat Contaminated with Fusarium

PubMed Central

Lim, Jongguk; Kim, Giyoung; Mo, Changyeun; Oh, Kyoungmin; Kim, Geonseob; Ham, Hyeonheui; Kim, Seongmin; Kim, Moon S.

2018-01-01

Fusarium is a common fungal disease in grains that reduces the yield of barley and wheat. In this study, a near infrared reflectance spectroscopic technique was used with a statistical prediction model to rapidly and non-destructively discriminate grain samples contaminated with Fusarium. Reflectance spectra were acquired from hulled barley, naked barley, and wheat samples contaminated with Fusarium using near infrared reflectance (NIR) spectroscopy with a wavelength range of 1175–2170 nm. After measurement, the samples were cultured in a medium to discriminate contaminated samples. A partial least square discrimination analysis (PLS-DA) prediction model was developed using the acquired reflectance spectra and the culture results. The correct classification rate (CCR) of Fusarium for the hulled barley, naked barley, and wheat samples developed using raw spectra was 98% or higher. The accuracy of discrimination prediction improved when second and third-order derivative pretreatments were applied. The grains contaminated with Fusarium could be rapidly discriminated using spectroscopy technology and a PLS-DA discrimination model, and the potential of the non-destructive discrimination method could be verified. PMID:29301319

Biomarkers for non-human primate type-I hypersensitivity: antigen-specific immunoglobulin E assays.

PubMed

Clark, Darcey; Shiota, Faith; Forte, Carla; Narayanan, Padma; Mytych, Daniel T; Hock, M Benjamin

2013-06-28

Immunoglobulin E (IgE) is the least abundant immunoglobulin in serum. However, development of an IgE immune response can induce IgE receptor-expressing cells to carry out potent effector functions. A reliable antigen-specific IgE biomarker method for use in non-human primate studies would facilitate (i) confirmation of Type-I hypersensitivity reactions during safety toxicology testing, and (ii) a better understanding of non-human primate models of allergic disease. We cloned and expressed a recombinant cynomolgus monkey IgE molecule in order to screen a panel of commercially available detection reagents raised against human IgE for cross-reactivity. The reagent most reactive to cynomolgus IgE was confirmed to be specific for IgE and did not bind recombinant cynomolgus monkey IgG1-4. A drug-specific IgE assay was developed on the MSD electrochemiluminescent (ECL) platform. The assay is capable of detecting 10 ng/mL drug-specific IgE. Importantly, the assay is able to detect IgE in the presence of excess IgG, the scenario likely to be present in a safety toxicology study. Using our ECL assay, we were able to confirm that serum from cynomolgus monkeys that had experienced clinical symptoms consistent with hypersensitivity responses contained IgE specific for a candidate therapeutic antibody. In addition, a bioassay for mast cell activation was developed using CD34(+)-derived cynomolgus monkey mast cells. This assay confirmed that plasma from animals identified as positive in the drug-specific IgE immunoassay contained biologically active IgE (i.e. could sensitize cultured mast cells), resulting in histamine release after exposure to the therapeutic antibody. These sensitive assays for Type-I hypersensitivity in the NHP can confirm that secondary events are downstream of immunogenicity. Copyright © 2013 Elsevier B.V. All rights reserved.

Non-destructive thermo-mechanical behavior assessment of glass-ceramics for dental applications

NASA Astrophysics Data System (ADS)

Kordatos, E. Z.; Abdulkadhim, Z.; Feteira, A. M.

2017-05-01

Every year millions of people seek dental treatment to either repair damaged, unaesthetic and dysfunctional teeth or replace missing natural teeth. Several dental materials have been developed to meet the stringent requirements in terms of mechanical properties, aesthetics and chemical durability in the oral environment. Glass-ceramics exhibit a suitable combination of these properties for dental restorations. This research is focused on the assessment of the thermomechanical behavior of bio-ceramics and particularly lithium aluminosilicate glass-ceramics (LAS glass-ceramics). Specifically, methodologies based on Infrared Thermography (IRT) have been applied in order the structure - property relationship to be evaluated. Non-crystallized, partially crystallized and fully crystallized glass-ceramic samples have been non-destructively assessed in order their thermo-mechanical behavior to be associated with their micro-structural features.

Data fusion for automated non-destructive inspection

PubMed Central

Brierley, N.; Tippetts, T.; Cawley, P.

2014-01-01

In industrial non-destructive evaluation (NDE), it is increasingly common for data acquisition to be automated, driving a recent substantial increase in the availability of data. The collected data need to be analysed, typically necessitating the painstaking manual labour of a skilled operator. Moreover, in automated NDE a region of an inspected component is typically interrogated several times, be it within a single data channel due to multiple probe passes, across several channels acquired simultaneously or over the course of repeated inspections. The systematic combination of these diverse readings is recognized to offer an opportunity to improve the reliability of the inspection, but is not achievable in a manual analysis. This paper describes a data-fusion-based software framework providing a partial automation capability, allowing component regions to be declared defect-free to a very high probability while readily identifying defect indications, thereby optimizing the use of the operator's time. The system is designed to applicable to a wide range of automated NDE scenarios, but the processing is exemplified using the industrial ultrasonic immersion inspection of aerospace turbine discs. Results obtained for industrial datasets demonstrate an orders-of-magnitude reduction in false-call rates, for a given probability of detection, achievable using the developed software system. PMID:25002828

Delayed gamma-ray spectroscopy with lanthanum bromide detector for non-destructive assay of nuclear material

DOE Office of Scientific and Technical Information (OSTI.GOV)

Favalli, Andrea; Iliev, Metodi; Ianakiev, Kiril

High-energy delayed γ-ray spectroscopy is a potential technique for directly assaying spent fuel assemblies and achieving the safeguards goal of quantifying nuclear material inventories for spent fuel handling, interim storage, reprocessing facilities, repository sites, and final disposal. Requirements for the γ-ray detection system, up to ~6 MeV, can be summarized as follows: high efficiency at high γ-ray energies, high energy resolution, good linearity between γ-ray energy and output signal amplitude, ability to operate at very high count rates, and ease of use in industrial environments such as nuclear facilities. High Purity Germanium Detectors (HPGe) are the state of the artmore » and provide excellent energy resolution but are limited in their count rate capability. Lanthanum Bromide (LaBr 3) scintillation detectors offer significantly higher count rate capabilities at lower energy resolution. Thus, LaBr 3 detectors may be an effective alternative for nuclear spent-fuel applications, where count-rate capability is a requirement. This paper documents the measured performance of a 2” (length) × 2” (diameter) of LaBr3 scintillation detector system, coupled to a negatively biased PMT and a tapered active high voltage divider, with count-rates up to ~3 Mcps. An experimental methodology was developed that uses the average current from the PMT’s anode and a dual source method to characterize the detector system at specific very high count rate values. Delayed γ-ray spectra were acquired with the LaBr 3 detector system at the Idaho Accelerator Center, Idaho State University, where samples of ~3g of 235U were irradiated with moderated neutrons from a photo-neutron source. Results of the spectroscopy characterization and analysis of the delayed γ-ray spectra acquired indicate the possible use of LaBr3 scintillation detectors when high count rate capability may outweigh the lower energy resolution.« less

Delayed gamma-ray spectroscopy with lanthanum bromide detector for non-destructive assay of nuclear material

DOE PAGES

Favalli, Andrea; Iliev, Metodi; Ianakiev, Kiril; ...

2017-10-09

High-energy delayed γ-ray spectroscopy is a potential technique for directly assaying spent fuel assemblies and achieving the safeguards goal of quantifying nuclear material inventories for spent fuel handling, interim storage, reprocessing facilities, repository sites, and final disposal. Requirements for the γ-ray detection system, up to ~6 MeV, can be summarized as follows: high efficiency at high γ-ray energies, high energy resolution, good linearity between γ-ray energy and output signal amplitude, ability to operate at very high count rates, and ease of use in industrial environments such as nuclear facilities. High Purity Germanium Detectors (HPGe) are the state of the artmore » and provide excellent energy resolution but are limited in their count rate capability. Lanthanum Bromide (LaBr 3) scintillation detectors offer significantly higher count rate capabilities at lower energy resolution. Thus, LaBr 3 detectors may be an effective alternative for nuclear spent-fuel applications, where count-rate capability is a requirement. This paper documents the measured performance of a 2” (length) × 2” (diameter) of LaBr3 scintillation detector system, coupled to a negatively biased PMT and a tapered active high voltage divider, with count-rates up to ~3 Mcps. An experimental methodology was developed that uses the average current from the PMT’s anode and a dual source method to characterize the detector system at specific very high count rate values. Delayed γ-ray spectra were acquired with the LaBr 3 detector system at the Idaho Accelerator Center, Idaho State University, where samples of ~3g of 235U were irradiated with moderated neutrons from a photo-neutron source. Results of the spectroscopy characterization and analysis of the delayed γ-ray spectra acquired indicate the possible use of LaBr3 scintillation detectors when high count rate capability may outweigh the lower energy resolution.« less

Delayed gamma-ray spectroscopy with lanthanum bromide detector for non-destructive assay of nuclear material

NASA Astrophysics Data System (ADS)

Favalli, Andrea; Iliev, Metodi; Ianakiev, Kiril; Hunt, Alan W.; Ludewigt, Bernhard

2018-01-01

High-energy delayed γ-ray spectroscopy is a potential technique for directly assaying spent fuel assemblies and achieving the safeguards goal of quantifying nuclear material inventories for spent fuel handling, interim storage, reprocessing facilities, repository sites, and final disposal. Requirements for the γ-ray detection system, up to ∼6 MeV, can be summarized as follows: high efficiency at high γ-ray energies, high energy resolution, good linearity between γ-ray energy and output signal amplitude, ability to operate at very high count rates, and ease of use in industrial environments such as nuclear facilities. High Purity Germanium Detectors (HPGe) are the state of the art and provide excellent energy resolution but are limited in their count rate capability. Lanthanum Bromide (LaBr3) scintillation detectors offer significantly higher count rate capabilities at lower energy resolution. Thus, LaBr3 detectors may be an effective alternative for nuclear spent-fuel applications, where count-rate capability is a requirement. This paper documents the measured performance of a 2" (length) × 2" (diameter) of LaBr3 scintillation detector system, coupled to a negatively biased PMT and a tapered active high voltage divider, with count-rates up to ∼3 Mcps. An experimental methodology was developed that uses the average current from the PMT's anode and a dual source method to characterize the detector system at specific very high count rate values. Delayed γ-ray spectra were acquired with the LaBr3 detector system at the Idaho Accelerator Center, Idaho State University, where samples of ∼3g of 235U were irradiated with moderated neutrons from a photo-neutron source. Results of the spectroscopy characterization and analysis of the delayed γ-ray spectra acquired indicate the possible use of LaBr3 scintillation detectors when high count rate capability may outweigh the lower energy resolution.

A diagnostic system for articular cartilage using non-destructive pulsed laser irradiation.

PubMed

Sato, Masato; Ishihara, Miya; Kikuchi, Makoto; Mochida, Joji

2011-07-01

Osteoarthritis involves dysfunction caused by cartilage degeneration, but objective evaluation methodologies based on the original function of the articular cartilage remain unavailable. Evaluations for osteoarthritis are mostly based simply on patient symptoms or the degree of joint space narrowing on X-ray images. Accurate measurement and quantitative evaluation of the mechanical characteristics of the cartilage is important, and the tissue properties of the original articular cartilage must be clarified to understand the pathological condition in detail and to correctly judge the efficacy of treatment. We have developed new methods to measure some essential properties of cartilage: a photoacoustic measurement method; and time-resolved fluorescence spectroscopy. A nanosecond-pulsed laser, which is completely non-destructive, is focused onto the target cartilage and induces a photoacoustic wave that will propagate with attenuation and is affected by the viscoelasticity of the surrounding cartilage. We also investigated whether pulsed laser irradiation and the measurement of excited autofluorescence allow real-time, non-invasive evaluation of tissue characteristics. The decay time, during which the amplitude of the photoacoustic wave is reduced by a factor of 1/e, represents the key numerical value used to characterize and evaluate the viscoelasticity and rheological behavior of the cartilage. Our findings show that time-resolved laser-induced autofluorescence spectroscopy (TR-LIFS) is useful for evaluating tissue-engineered cartilage. Photoacoustic measurement and TR-LIFS, predicated on the interactions between optics and living organs, is a suitable methodology for diagnosis during arthroscopy, allowing quantitative and multidirectional evaluation of the original function of the cartilage based on a variety of parameters. Copyright © 2011 Wiley-Liss, Inc.

Thermal Transmission through Existing Building Enclosures: Destructive Monitoring in Intermediate Layers versus Non-Destructive Monitoring with Sensors on Surfaces

PubMed Central

Echarri, Víctor; Espinosa, Almudena; Rizo, Carlos

2017-01-01

Opaque enclosures of buildings play an essential role in the level of comfort experienced indoors and annual energy demand. The impact of solar radiation and thermal inertia of the materials that make up the multi-layer enclosures substantially modify thermal transmittance behaviour of the enclosures. This dynamic form of heat transfer, additionally affected by indoor HVAC systems, has a substantial effect on the parameters that define comfort. It also has an impact on energy demand within a daily cycle as well as throughout a one-year use cycle. This study describes the destructive monitoring of an existing block of flats located in Alicante. Once the enclosure was opened, sensors of temperature (PT100), air velocity, and relative humidity were located in the different layers of the enclosure, as well as in the interior and exterior surfaces. A pyranometer was also installed to measure solar radiation levels. A temperature data correction algorithm was drawn up to address irregularities produced in the enclosure. The algorithm was applied using a Raspberry Pi processor in the data collection system. The comparative results of temperature gradients versus non-destructive monitoring systems are presented, providing measures of the transmittance value, surface temperatures and indoor and outdoor air temperatures. This remote sensing system can be used in future studies to quantify and compare the energy savings of different enclosure construction solutions. PMID:29292781

Thermal Transmission through Existing Building Enclosures: Destructive Monitoring in Intermediate Layers versus Non-Destructive Monitoring with Sensors on Surfaces.

PubMed

Echarri, Víctor; Espinosa, Almudena; Rizo, Carlos

2017-12-08

Opaque enclosures of buildings play an essential role in the level of comfort experienced indoors and annual energy demand. The impact of solar radiation and thermal inertia of the materials that make up the multi-layer enclosures substantially modify thermal transmittance behaviour of the enclosures. This dynamic form of heat transfer, additionally affected by indoor HVAC systems, has a substantial effect on the parameters that define comfort. It also has an impact on energy demand within a daily cycle as well as throughout a one-year use cycle. This study describes the destructive monitoring of an existing block of flats located in Alicante. Once the enclosure was opened, sensors of temperature (PT100), air velocity, and relative humidity were located in the different layers of the enclosure, as well as in the interior and exterior surfaces. A pyranometer was also installed to measure solar radiation levels. A temperature data correction algorithm was drawn up to address irregularities produced in the enclosure. The algorithm was applied using a Raspberry Pi processor in the data collection system. The comparative results of temperature gradients versus non-destructive monitoring systems are presented, providing measures of the transmittance value, surface temperatures and indoor and outdoor air temperatures. This remote sensing system can be used in future studies to quantify and compare the energy savings of different enclosure construction solutions.

Assessment of statistic analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with alpha-hexylcinnamic aldehyde as an example.

PubMed

Takeyoshi, Masahiro; Sawaki, Masakuni; Yamasaki, Kanji; Kimber, Ian

2003-09-30

The murine local lymph node assay (LLNA) is used for the identification of chemicals that have the potential to cause skin sensitization. However, it requires specific facility and handling procedures to accommodate a radioisotopic (RI) endpoint. We have developed non-radioisotopic (non-RI) endpoint of LLNA based on BrdU incorporation to avoid a use of RI. Although this alternative method appears viable in principle, it is somewhat less sensitive than the standard assay. In this study, we report investigations to determine the use of statistical analysis to improve the sensitivity of a non-RI LLNA procedure with alpha-hexylcinnamic aldehyde (HCA) in two separate experiments. Consequently, the alternative non-RI method required HCA concentrations of greater than 25% to elicit a positive response based on the criterion for classification as a skin sensitizer in the standard LLNA. Nevertheless, dose responses to HCA in the alternative method were consistent in both experiments and we examined whether the use of an endpoint based upon the statistical significance of induced changes in LNC turnover, rather than an SI of 3 or greater, might provide for additional sensitivity. The results reported here demonstrate that with HCA at least significant responses were, in each of two experiments, recorded following exposure of mice to 25% of HCA. These data suggest that this approach may be more satisfactory-at least when BrdU incorporation is measured. However, this modification of the LLNA is rather less sensitive than the standard method if employing statistical endpoint. Taken together the data reported here suggest that a modified LLNA in which BrdU is used in place of radioisotope incorporation shows some promise, but that in its present form, even with the use of a statistical endpoint, lacks some of the sensitivity of the standard method. The challenge is to develop strategies for further refinement of this approach.

Two non-invasive diagnostic tools for invasive aspergilosis: (1-3)-beta-D-glucan and the galactomannan assay.

PubMed

Kelaher, Amy

2006-01-01

Invasive aspergillosis (IA) is a serious cause of morbidity and mortality among immunocompromised patients. Prompt and non-invasive methods for diagnosing IA are needed to improve the management of this life-threatening infection in patients with hematological disorders. In summary, this retrospective review of studies performed on the two assays finds that both assays have high sensitivity and specificity but are more useful when used together as a diagnostic strategy for patients with invasive aspergillosis.

Method and apparatus for low temperature destruction of halogenated hydrocarbons

DOEpatents

Reagen, William Kevin; Janikowski, Stuart Kevin

1999-01-01

A method and apparatus for decomposing halogenated hydrocarbons are provided. The halogenated hydrocarbon is mixed with solvating agents and maintained in a predetermined atmosphere and at a predetermined temperature. The mixture is contacted with recyclable reactive material for chemically reacting with the recyclable material to create dehalogenated hydrocarbons and halogenated inorganic compounds. A feature of the invention is that the process enables low temperature destruction of halogenated hydrocarbons.

Nondestructive Assay Data Integration with the SKB-50 Assemblies - FY16 Update

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tobin, Stephen Joseph; Fugate, Michael Lynn; Trellue, Holly Renee

2016-10-28

A project to research the application of non-destructive assay (NDA) techniques for spent fuel assemblies is underway at the Central Interim Storage Facility for Spent Nuclear Fuel (for which the Swedish acronym is Clab) in Oskarshamn, Sweden. The research goals of this project contain both safeguards and non-safeguards interests. These nondestructive assay (NDA) technologies are designed to strengthen the technical toolkit of safeguard inspectors and others to determine the following technical goals more accurately; Verify initial enrichment, burnup, and cooling time of facility declaration for spent fuel assemblies; Detect replaced or missing pins from a given spent fuel assembly tomore » confirm its integrity; and Estimate plutonium mass and related plutonium and uranium fissile mass parameters in spent fuel assemblies. Estimate heat content, and measure reactivity (multiplication).« less

Craton destruction and related resources

NASA Astrophysics Data System (ADS)

Zhu, Rixiang; Zhang, Hongfu; Zhu, Guang; Meng, Qingren; Fan, Hongrui; Yang, Jinhui; Wu, Fuyuan; Zhang, Zhiyong; Zheng, Tianyu

2017-10-01

Craton destruction is a dynamic event that plays an important role in Earth's evolution. Based on comprehensive observations of many studies on the North China Craton (NCC) and correlations with the evolution histories of other cratons around the world, craton destruction has be defined as a geological process that results in the total loss of craton stability due to changes in the physical and chemical properties of the involved craton. The mechanisms responsible for craton destruction would be as the follows: (1) oceanic plate subduction; (2) rollback and retreat of a subducting oceanic plate; (3) stagnation and dehydration of a subducting plate in the mantle transition zone; (4) melting of the mantle above the mantle transition zone caused by dehydration of a stagnant slab; (5) non-steady flow in the upper mantle induced by melting, and/or (6) changes in the nature of the lithospheric mantle and consequent craton destruction caused by non-steady flow. Oceanic plate subduction itself does not result in craton destruction. For the NCC, it is documented that westward subduction of the paleo-Pacific plate should have initiated at the transition from the Middle-to-Late Jurassic, and resulted in the change of tectonic regime of eastern China. We propose that subduction, rollback and retreat of oceanic plates and dehydration of stagnant slabs are the main dynamic factors responsible for both craton destruction and concentration of mineral deposits, such as gold, in the overriding continental plate. Based on global distribution of gold deposits, we suggest that convergent plate margins are the most important setting for large gold concentrations. Therefore, decratonic gold deposits appear to occur preferentially in regions with oceanic subduction and overlying continental lithospheric destruction/modification/growth.

Non-contact method for directing electrotaxis

NASA Astrophysics Data System (ADS)

Ahirwar, Dinesh K.; Nasser, Mohd W.; Jones, Travis H.; Sequin, Emily K.; West, Joseph D.; Henthorne, Timothy L.; Javor, Joshua; Kaushik, Aniruddha M.; Ganju, Ramesh K.; Subramaniam, Vish V.

2015-06-01

We present a method to induce electric fields and drive electrotaxis (galvanotaxis) without the need for electrodes to be in contact with the media containing the cell cultures. We report experimental results using a modification of the transmembrane assay, demonstrating the hindrance of migration of breast cancer cells (SCP2) when an induced a.c. electric field is present in the appropriate direction (i.e. in the direction of migration). Of significance is that migration of these cells is hindered at electric field strengths many orders of magnitude (5 to 6) below those previously reported for d.c. electrotaxis, and even in the presence of a chemokine (SDF-1α) or a growth factor (EGF). Induced a.c. electric fields applied in the direction of migration are also shown to hinder motility of non-transformed human mammary epithelial cells (MCF10A) in the presence of the growth factor EGF. In addition, we also show how our method can be applied to other cell migration assays (scratch assay), and by changing the coil design and holder, that it is also compatible with commercially available multi-well culture plates.

Non-Destructive Study of Bulk Crystallinity and Elemental Composition of Natural Gold Single Crystal Samples by Energy-Resolved Neutron Imaging

PubMed Central

Tremsin, Anton S.; Rakovan, John; Shinohara, Takenao; Kockelmann, Winfried; Losko, Adrian S.; Vogel, Sven C.

2017-01-01

Energy-resolved neutron imaging enables non-destructive analyses of bulk structure and elemental composition, which can be resolved with high spatial resolution at bright pulsed spallation neutron sources due to recent developments and improvements of neutron counting detectors. This technique, suitable for many applications, is demonstrated here with a specific study of ~5–10 mm thick natural gold samples. Through the analysis of neutron absorption resonances the spatial distribution of palladium (with average elemental concentration of ~0.4 atom% and ~5 atom%) is mapped within the gold samples. At the same time, the analysis of coherent neutron scattering in the thermal and cold energy regimes reveals which samples have a single-crystalline bulk structure through the entire sample volume. A spatially resolved analysis is possible because neutron transmission spectra are measured simultaneously on each detector pixel in the epithermal, thermal and cold energy ranges. With a pixel size of 55 μm and a detector-area of 512 by 512 pixels, a total of 262,144 neutron transmission spectra are measured concurrently. The results of our experiments indicate that high resolution energy-resolved neutron imaging is a very attractive analytical technique in cases where other conventional non-destructive methods are ineffective due to sample opacity. PMID:28102285

Comparative testing of radiographic testing, ultrasonic testing and phased array advanced ultrasonic testing non destructive testing techniques in accordance with the AWS D1.5 bridge welding code.

DOT National Transportation Integrated Search

2014-02-01

A comprehensive body of non-destructive testing data was collected from steel bridge welds under real-world conditions in a fabricators shop. Three different non-destructive testing (NDT) techniques were used on each weld inspection, these being R...

The microculture tetrazolium assay (MTA): another colorimetric method of testing Plasmodium falciparum chemosensitivity.

PubMed

Delhaes, L; Lazaro, J E; Gay, F; Thellier, M; Danis, M

1999-01-01

Malarial lactate dehydrogenase (LDH), which uses 3-acetyl pyridine adenine dinucleotide as coenzyme in a reaction leading to the formation of pyruvate from L-lactate, may be used to study the susceptibility of Plasmodium falciparum to a drug in vitro. Several methods to determine the activity of this enzyme are available. One, the colorimetric method of Makler and colleagues, was modified slightly, by using sodium-2,3-bis-[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5 - carboxanilide (XTT) and following the reaction by measuring the optical density at 450 nm. Using two, culture-adapted strains of P. falciparum, this LDH assay was compared with the unmodified Makler's assay and with the isotopic microtest based on the incorporation of tritium-labelled hypoxanthine. Fresh, clinical P. falciparum isolates were also tested in the presence of several drugs, including chloroquine, mefloquine, quinine, halofantrine, atovaquone and qinghaosu derivatives. The results of the three assays were correlated for all the drugs tested except atovaquone. The two enzymatic assays are non-radioactive, rapid, reliable, inexpensive to perform and semi-automatic. However, they do require an initial parasitaemia of 2% with a haematocrit of 1.8%.

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

DOEpatents

Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.

2016-04-05

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

DOEpatents

Schaff, Ulrich Y; Koh, Chung-Yan; Sommer, Gregory J

2015-02-24

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Thermal history sensors for non-destructive temperature measurements in harsh environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pilgrim, C. C.; Heyes, A. L.; Feist, J. P.

2014-02-18

The operating temperature is a critical physical parameter in many engineering applications, however, can be very challenging to measure in certain environments, particularly when access is limited or on rotating components. A new quantitative non-destructive temperature measurement technique has been proposed which relies on thermally induced permanent changes in ceramic phosphors. This technique has several distinct advantages over current methods for many different applications. The robust ceramic material stores the temperature information allowing long term thermal exposures in harsh environment to be measured at a convenient time. Additionally, rare earth dopants make the ceramic phosphorescent so that the temperature informationmore » can be interpreted by automated interrogation of the phosphorescent light. This technique has been demonstrated by application of YAG doped with dysprosium and europium as coatings through the air-plasma spray process. Either material can be used to measure temperature over a wide range, namely between 300°C and 900°C. Furthermore, results show that the material records the peak exposure temperature and prolonged exposure at lower temperatures would have no effect on the temperature measurement. This indicates that these materials could be used to measure peak operating temperatures in long-term testing.« less

Excreted thiocyanate detects live reef fishes illegally collected using cyanide--a non-invasive and non-destructive testing approach.

PubMed

Vaz, Marcela C M; Rocha-Santos, Teresa A P; Rocha, Rui J M; Lopes, Isabel; Pereira, Ruth; Duarte, Armando C; Rubec, Peter J; Calado, Ricardo

2012-01-01

Cyanide fishing is a method employed to capture marine fish alive on coral reefs. They are shipped to markets for human consumption in Southeast Asia, as well as to supply the marine aquarium trade worldwide. Although several techniques can be used to detect cyanide in reef fish, there is still no testing method that can be used to survey the whole supply chain. Most methods for cyanide detection are time-consuming and require the sacrifice of the sampled fish. Thiocyanate anion (SCN(-)) is a metabolite produced by the main metabolic pathway for cyanide anion (CN(-)) detoxification. Our study employed an optical fiber (OF) methodology (analytical time <6 min) to detect SCN(-) in a non-invasive and non-destructive manner. Our OF methodology is able to detect trace levels (>3.16 µg L(-1)) of SCN(-) in seawater. Given that marine fish exposed to cyanide excrete SCN(-) in the urine, elevated levels of SCN(-) present in the seawater holding live reef fish indicate that the surveyed specimens were likely exposed to cyanide. In our study, captive-bred clownfish (Amphiprion clarkii) pulse exposed for 60 s to either 12.5 or 25 mg L(-1) of CN(-) excreted up to 6.96±0.03 and 9.84±0.03 µg L(-1) of SCN(-), respectively, during the 28 days following exposure. No detectable levels of SCN(-) were recorded in the water holding control organisms not exposed to CN(-), or in synthetic seawater lacking fish. While further research is necessary, our methodology can allow a rapid detection of SCN(-) in the holding water and can be used as a screening tool to indicate if live reef fish were collected with cyanide.

Excreted Thiocyanate Detects Live Reef Fishes Illegally Collected Using Cyanide—A Non-Invasive and Non-Destructive Testing Approach

PubMed Central

Vaz, Marcela C. M.; Rocha-Santos, Teresa A. P.; Rocha, Rui J. M.; Lopes, Isabel; Pereira, Ruth; Duarte, Armando C.; Rubec, Peter J.; Calado, Ricardo

2012-01-01

Cyanide fishing is a method employed to capture marine fish alive on coral reefs. They are shipped to markets for human consumption in Southeast Asia, as well as to supply the marine aquarium trade worldwide. Although several techniques can be used to detect cyanide in reef fish, there is still no testing method that can be used to survey the whole supply chain. Most methods for cyanide detection are time-consuming and require the sacrifice of the sampled fish. Thiocyanate anion (SCN−) is a metabolite produced by the main metabolic pathway for cyanide anion (CN−) detoxification. Our study employed an optical fiber (OF) methodology (analytical time <6 min) to detect SCN− in a non-invasive and non-destructive manner. Our OF methodology is able to detect trace levels (>3.16 µg L−1) of SCN− in seawater. Given that marine fish exposed to cyanide excrete SCN− in the urine, elevated levels of SCN− present in the seawater holding live reef fish indicate that the surveyed specimens were likely exposed to cyanide. In our study, captive-bred clownfish (Amphiprion clarkii) pulse exposed for 60 s to either 12.5 or 25 mg L−1 of CN− excreted up to 6.96±0.03 and 9.84±0.03 µg L−1 of SCN−, respectively, during the 28 days following exposure. No detectable levels of SCN− were recorded in the water holding control organisms not exposed to CN−, or in synthetic seawater lacking fish. While further research is necessary, our methodology can allow a rapid detection of SCN− in the holding water and can be used as a screening tool to indicate if live reef fish were collected with cyanide. PMID:22536375

Non-destructive quantification of pharmaceutical tablet coatings using terahertz pulsed imaging and optical coherence tomography

NASA Astrophysics Data System (ADS)

Zhong, Shuncong; Shen, Yao-Chun; Ho, Louise; May, Robert K.; Zeitler, J. Axel; Evans, Mike; Taday, Philip F.; Pepper, Michael; Rades, Thomas; Gordon, Keith C.; Müller, Ronny; Kleinebudde, Peter

2011-03-01

Optical coherence tomography (OCT) and terahertz pulsed imaging (TPI) are two powerful techniques allowing high quality cross-sectional images from within scattering media to be obtained non-destructively. In this paper, we report experimental results of using OCT and TPI for quantitatively characterizing pharmaceutical tablet coatings in the thickness range of 10-140 μm. We found that the spectral OCT system developed in-house has an axial resolution of 0.9 μm, and is capable of quantifying very thin coatings in the range of 10-60 μm. The upper limit of 60 μm within the tablet coating and core is owed to the strong scattering of OCT light, which has relatively short wavelengths in the range of 0.5-1.0 μm. On the other hand, TPI utilizes terahertz radiation that has substantially long wavelengths in the range of hundreds of microns, and thus is less prone to the scattering problem. Consequently TPI has been demonstrated to be able to quantify thicker coatings in the range of 40-140 μm and beyond. We concluded that OCT and TPI are two complementary analytical techniques for non-destructive and quantitative characterization of pharmaceutical tablet coatings.

System and method for assaying radiation

DOEpatents

DiPrete, David P; Whiteside, Tad; Pak, Donald J; DiPrete, Cecilia C

2013-11-12

A system for assaying radiation includes a sample holder configured to hold a liquid scintillation solution. A photomultiplier receives light from the liquid scintillation solution and generates a signal reflective of the light. A control circuit biases the photomultiplier and receives the signal from the photomultiplier reflective of the light. A light impermeable casing surrounds the sample holder, photomultiplier, and control circuit. A method for assaying radiation includes placing a sample in a liquid scintillation solution, placing the liquid scintillation solution in a sample holder, and placing the sample holder inside a light impermeable casing. The method further includes positioning a photomultiplier inside the light impermeable casing and supplying power to a control circuit inside the light impermeable casing.

Non-Destructive Current Sensing for Energy Efficiency Monitoring in Buildings with Environmental Certification.

PubMed

Mota, Lia Toledo Moreira; Mota, Alexandre de Assis; Coiado, Lorenzo Campos

2015-07-10

Nowadays, buildings environmental certifications encourage the implementation of initiatives aiming to increase energy efficiency in buildings. In these certification systems, increased energy efficiency arising from such initiatives must be demonstrated. Thus, a challenge to be faced is how to check the increase in energy efficiency related to each of the employed initiatives without a considerable building retrofit. In this context, this work presents a non-destructive method for electric current sensing to assess implemented initiatives to increase energy efficiency in buildings with environmental certification. This method proposes the use of a sensor that can be installed directly in the low voltage electrical circuit conductors that are powering the initiative under evaluation, without the need for reforms that result in significant costs, repair, and maintenance. The proposed sensor consists of three elements: an air-core transformer current sensor, an amplifying/filtering stage, and a microprocessor. A prototype of the proposed sensor was developed and tests were performed to validate this sensor. Based on laboratory tests, it was possible to characterize the proposed current sensor with respect to the number of turns and cross-sectional area of the primary and secondary coils. Furthermore, using the Least Squares Method, it was possible to determine the efficiency of the air core transformer current sensor (the best efficiency found, considering different test conditions, was 2%), which leads to a linear output response.

Non-Destructive Current Sensing for Energy Efficiency Monitoring in Buildings with Environmental Certification

PubMed Central

Mota, Lia Toledo Moreira; Mota, Alexandre de Assis; Coiado, Lorenzo Campos

2015-01-01

Nowadays, buildings environmental certifications encourage the implementation of initiatives aiming to increase energy efficiency in buildings. In these certification systems, increased energy efficiency arising from such initiatives must be demonstrated. Thus, a challenge to be faced is how to check the increase in energy efficiency related to each of the employed initiatives without a considerable building retrofit. In this context, this work presents a non-destructive method for electric current sensing to assess implemented initiatives to increase energy efficiency in buildings with environmental certification. This method proposes the use of a sensor that can be installed directly in the low voltage electrical circuit conductors that are powering the initiative under evaluation, without the need for reforms that result in significant costs, repair, and maintenance. The proposed sensor consists of three elements: an air-core transformer current sensor, an amplifying/filtering stage, and a microprocessor. A prototype of the proposed sensor was developed and tests were performed to validate this sensor. Based on laboratory tests, it was possible to characterize the proposed current sensor with respect to the number of turns and cross-sectional area of the primary and secondary coils. Furthermore, using the Least Squares Method, it was possible to determine the efficiency of the air core transformer current sensor (the best efficiency found, considering different test conditions, was 2%), which leads to a linear output response. PMID:26184208

Non-invasive Assessments of Adipose Tissue Metabolism In Vitro.

PubMed

Abbott, Rosalyn D; Borowsky, Francis E; Quinn, Kyle P; Bernstein, David L; Georgakoudi, Irene; Kaplan, David L

2016-03-01

Adipose tissue engineering is a diverse area of research where the developed tissues can be used to study normal adipose tissue functions, create disease models in vitro, and replace soft tissue defects in vivo. Increasing attention has been focused on the highly specialized metabolic pathways that regulate energy storage and release in adipose tissues which affect local and systemic outcomes. Non-invasive, dynamic measurement systems are useful to track these metabolic pathways in the same tissue model over time to evaluate long term cell growth, differentiation, and development within tissue engineering constructs. This approach reduces costs and time in comparison to more traditional destructive methods such as biochemical and immunochemistry assays and proteomics assessments. Towards this goal, this review will focus on important metabolic functions of adipose tissues and strategies to evaluate them with non-invasive in vitro methods. Current non-invasive methods, such as measuring key metabolic markers and endogenous contrast imaging will be explored.

Non-destructive Analysis of Oil-Contaminated Soil Core Samples by X-ray Computed Tomography and Low-Field Nuclear Magnetic Resonance Relaxometry: a Case Study

PubMed Central

Mitsuhata, Yuji; Nishiwaki, Junko; Kawabe, Yoshishige; Utsuzawa, Shin; Jinguuji, Motoharu

2010-01-01

Non-destructive measurements of contaminated soil core samples are desirable prior to destructive measurements because they allow obtaining gross information from the core samples without touching harmful chemical species. Medical X-ray computed tomography (CT) and time-domain low-field nuclear magnetic resonance (NMR) relaxometry were applied to non-destructive measurements of sandy soil core samples from a real site contaminated with heavy oil. The medical CT visualized the spatial distribution of the bulk density averaged over the voxel of 0.31 × 0.31 × 2 mm3. The obtained CT images clearly showed an increase in the bulk density with increasing depth. Coupled analysis with in situ time-domain reflectometry logging suggests that this increase is derived from an increase in the water volume fraction of soils with depth (i.e., unsaturated to saturated transition). This was confirmed by supplementary analysis using high-resolution micro-focus X-ray CT at a resolution of ∼10 μm, which directly imaged the increase in pore water with depth. NMR transverse relaxation waveforms of protons were acquired non-destructively at 2.7 MHz by the Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence. The nature of viscous petroleum molecules having short transverse relaxation times (T2) compared to water molecules enabled us to distinguish the water-saturated portion from the oil-contaminated portion in the core sample using an M0–T2 plot, where M0 is the initial amplitude of the CPMG signal. The present study demonstrates that non-destructive core measurements by medical X-ray CT and low-field NMR provide information on the groundwater saturation level and oil-contaminated intervals, which is useful for constructing an adequate plan for subsequent destructive laboratory measurements of cores. PMID:21258437

Screening of anthocyanins in single red grapes using a non-destructive method based on the near infrared hyperspectral technology and chemometrics.

PubMed

Martínez-Sandoval, Jesús Raúl; Nogales-Bueno, Julio; Rodríguez-Pulido, Francisco José; Hernández-Hierro, José Miguel; Segovia-Quintero, Manuel Alberto; Martínez-Rosas, Miguel Enrique; Heredia, Francisco José

2016-03-30

Anthocyanins are the main compounds responsible for the colour of red wines and therefore it may be important to evaluate the content of the aforesaid secondary metabolites during grape ripening due to the crucial importance to determine wine colour. Nowadays, there is a growing demand of rapid and non-destructive analytical tools for analysing grapes, such as the emerging hyperspectral analysis. The hyperspectral images of intact grapes (Vitis vinifera L. cv. Tempranillo, Graciano, Garnacha and Mazuelo red grape from vineyards located in the D.O.Ca. Rioja at two different developmental stages) were recorded using a near infrared hyperspectral imaging device (900-1700 nm). Reference values of anthocyanins were obtained by HPLC-DAD. Calibrations were performed by modified partial least squares regression and present a good potential (coefficient of determination of 0.72 and standard error of cross-validation values of 0.78 and 0.70 mg per grape for total and non-acylated anthocyanins respectively). The procedure reported here presents a good potential for a fast and reasonably inexpensive screening of these compounds in intact single berries. Moreover, the heterogeneity of anthocyanins within the same ripeness stage could be evaluated using this non-detructive tool. © 2015 Society of Chemical Industry.

Non-destructive testing techniques for the forensic engineering investigation of reinforced concrete buildings.

PubMed

Hobbs, Brian; Tchoketch Kebir, Mohamed

2007-04-11

This study describes in detail the results of a laboratory investigation where the compressive strength of 150mm side-length cubes was evaluated. Non-destructive testing (NDT) was carried out using ultrasonic pulse velocity (UPV) and impact rebound hammer (IRH) techniques to establish a correlation with the compressive strengths of compression tests. To adapt the Schmidt hammer apparatus and the ultrasonic pulse velocity tester to the type of concrete used in Algeria, concrete mix proportions that are recommended by the Algerian code were chosen. The resulting correlation curve for each test is obtained by changing the level of compaction, water/cement ratio and concrete age of specimens. Unlike other works, the research highlights the significant effect of formwork material on surface hardness of concrete where two different mould materials for specimens were used (plastic and wood). A combined method for the above two tests, reveals an improvement in the strength estimation of concrete. The latter shows more improvement by including the concrete density. The resulting calibration curves for strength estimation were compared with others from previous published literature.

Study of Lamb Waves for Non-Destructive Testing Behind Screens

NASA Astrophysics Data System (ADS)

Kauffmann, P.; Ploix, M.-A.; Chaix, J.-F.; Gueudré, C.; Corneloup, G.; Baqué, F. AF(; )

2018-01-01

The inspection and control of sodium-cooled fast reactors (SFR) is a major issue for the nuclear industry. Ultrasonic solutions are under study because of the opacity of liquid sodium. In this paper, the use of leaky Lamb waves is considered for non-destructive testing (NDT) on parallel and immersed structures assimilated as plates. The first phase of our approach involved studying the propagation properties of leaky Lamb waves. Equations that model the propagation of Lamb waves in an immersed plate were solved numerically. The phase velocity can be experimentally measured using a two dimensional Fourier transform. The group velocity can be experimentally measured using a short-time Fourier transform technique. Attenuation of leaky Lamb waves is mostly due to the re-emission of energy into the surrounding fluid, and it can be measured by these two techniques.

Automatic non-destructive system for quality assurance of welded elements in the aircraft industry

NASA Astrophysics Data System (ADS)

Chady, Tomasz; Waszczuk, Paweł; Szydłowski, Michał; Szwagiel, Mariusz

2018-04-01

Flaws that might be a result of the welding process have to be detected, in order to assure high quality thus reliability of elements exploited in aircraft industry. Currently the inspection stage is conducted manually by a qualified workforce. There are no commercially available systems that could support or replace humans in the flaw detection process. In this paper authors present a novel non-destructive system developed for quality assurance purposes of welded elements utilized in the aircraft industry.

Application of golay complementary coded excitation schemes for non-destructive testing of sandwich structures

NASA Astrophysics Data System (ADS)

Arora, Vanita; Mulaveesala, Ravibabu

2017-06-01

In recent years, InfraRed Thermography (IRT) has become a widely accepted non-destructive testing technique to evaluate the structural integrity of composite sandwich structures due to its full-field, remote, fast and in-service inspection capabilities. This paper presents a novel infrared thermographic approach named as Golay complementary coded thermal wave imaging is presented to detect disbonds in a sandwich structure having face sheets from Glass/Carbon Fibre Reinforced (GFR/CFR) laminates and core of the wooden block.

Non-destructive hyperspectral imaging of quarantined Mars Returned Samples

NASA Astrophysics Data System (ADS)

Simionovici, Alexandre; Viso, Michel; Beck, Pierre; Lemelle, Laurence; Westphal, Andrew; Vincze, Laszlo; Schoonjans, Tom; Fihman, Francois; Chazalnoel, Pascale; Ferroir, Tristan; Solé, Vicente Armando; Tucoulou, R.

Introduction: In preparation for the upcoming International Mars Sample Return mission (MSR), returning samples containing potential biohazards, we have implemented a hyperspec-tral method of in-situ analysis of grains performed in BSL4 quarantine conditions, by combining several non-destructive imaging diagnostics. This allows sample transportation on optimized experimental setups, while monitoring the sample quarantine conditions. Our hyperspectral methodology was tested during analyses of meteorites [1-2] and cometary and interstellar grains from the recent NASA Stardust mission [3-6]. Synchrotron Radiation protocols: X-ray analysis methods are widely accepted as the least destructive probes of fragile, unique samples. Diffraction, X-ray fluorescence and ab-sorption micro/nano-spectroscopies were performed on chondritic test samples using focused monochromatic beams at the ESRF synchrotron in Grenoble, France. 2D maps of grain com-position down to ppm concentrations and polycrystalline structure have simultaneously been acquired, followed by X-ray absorption performed on elements of Z 26. Ideally, absorption micro-tomography can later be performed in full-beam mode to record the 3D morphology of the grain followed by fluorescence-tomography in focus-beam mode which complements this picture with a 3D elemental image of the grain. Lab-based protocols: Raman and IR-based spectroscopies have been performed in reflection mode for mineralogical imaging of the grains in the laboratory using commercial microscopes. The spatial resolution varied in the 1-10 m range. Laser limited penetration of opaque samples permits only 2D imaging of the few nanometer-thick outer layers of the grains. Mineralogical maps are now routinely acquired using Raman spectroscopy at sub-micron scales through the 3 container walls of the Martian sample holder, followed by IR few-micrometer spot measurements recording C-based and potential aqueous alteration distributions. Sample Holder: A

Application of optical interferometric techniques for non-destructive evaluation of novel "green" composite materials

NASA Astrophysics Data System (ADS)

Pagliarulo, Vito; Russo, Pietro; Bianco, Vittorio; Ferraro, Pietro; Simeoli, Giorgio; Cimino, Francesca; Ruggiero, Berardo

2018-04-01

Nowadays the use of advanced composite materials in aeronautics, both civil and military, in automotive and in sport applications, citing some, is well established. The characteristics of composite materials in terms of weight, fatigue resistance and corrosion resistance make them competitive with respect to conventional ones. On the other side, the fabrication process of the most employed composites reinforced by carbon fibers or glass fibers, needs of complex steps that not always are environmental complaisant. Moreover, such fibers are not themselves "green". For these reasons, in the last decades, the use of natural reinforcing fibers has gained an increasing attention allowing the development of new materials with the same advantages of composite systems but respecting the environment. Furthermore, such materials for their structural complexity are not always compatible with the use of standard non-destructive evaluation as the ultrasounds methods. In this work the efficiency of the employment of optical interferometric techniques as nondestructive evaluation methods in full field modality is proved on novel "green" composite materials. In particular, Electronic Speckle Pattern Interferometry has been tested on different kinds of specimens after flexural tests.

Non-destructive evaluation of specialty coating degradation using terahertz time-domain spectroscopy

NASA Astrophysics Data System (ADS)

Nicoletti, Carley R.; Cramer, Laura; Fletcher, Alan; Zimdars, David; Iqbal, Zafar; Federici, John F.

2017-05-01

The Terahertz Time Domain Reflection Spectroscopy (THz-TDS) method of paint layer diagnostics is a non-contact electromagnetic technique analogous to pulsed-ultrasound with the added capability of spectroscopic characterization. The THz-TDS sensor emits a near-single cycle electromagnetic pulse with a bandwidth from 0.1 to 3 THz. This wide bandwidth pulse is focused on the coating, and echo pulses are generated from each interface (air-coating, layer-layer, coating-substrate). In this paper, the THz-TDS method is applied to specialty aircraft coatings. The THz-TDS method is able to penetrate the whole coating stack and sample the properties of each layer. Because the reflected pulses from individual layers typically overlap in time, the complex permittivity function and thickness of each layer is determined by a best fit of the measured reflection (either in time or frequency domain) to a layered model of the paint. The THz- TDS method is applied to specialty coatings prior to and during accelerated aging on a series of test coupons. The coupons are also examined during aging using ATR (attenuated total reflectance)-FTIR spectroscopy, Raman scattering spectroscopy, and Scanning Electron Microscopy (SEM) to ascertain, quantify, and understand the breakdown mechanisms of the coatings. In addition, the same samples are characterized using THz-TDS techniques to determine if the THz-TDS method can be utilized as a non-destructive evaluation technique to sense degradation of the coatings. Our results suggest that the degradation mechanism begins in the top coat layer. In this layer, 254 nm UV illumination in combination with the presence of moisture works partially with oxides as catalysts to decompose the polymer matrix thereby creating porosity in the top coat layer. Since the catalytic effect is partial, loss of the oxides by chemical reaction can also occur. As the topcoat layer becomes more porous, it allows water vapor to permeate the topcoat layer and interact

Application of NIRS coupled with PLS regression as a rapid, non-destructive alternative method for quantification of KBA in Boswellia sacra

NASA Astrophysics Data System (ADS)

Al-Harrasi, Ahmed; Rehman, Najeeb Ur; Mabood, Fazal; Albroumi, Muhammaed; Ali, Liaqat; Hussain, Javid; Hussain, Hidayat; Csuk, René; Khan, Abdul Latif; Alam, Tanveer; Alameri, Saif

2017-09-01

In the present study, for the first time, NIR spectroscopy coupled with PLS regression as a rapid and alternative method was developed to quantify the amount of Keto-β-Boswellic Acid (KBA) in different plant parts of Boswellia sacra and the resin exudates of the trunk. NIR spectroscopy was used for the measurement of KBA standards and B. sacra samples in absorption mode in the wavelength range from 700-2500 nm. PLS regression model was built from the obtained spectral data using 70% of KBA standards (training set) in the range from 0.1 ppm to 100 ppm. The PLS regression model obtained was having R-square value of 98% with 0.99 corelationship value and having good prediction with RMSEP value 3.2 and correlation of 0.99. It was then used to quantify the amount of KBA in the samples of B. sacra. The results indicated that the MeOH extract of resin has the highest concentration of KBA (0.6%) followed by essential oil (0.1%). However, no KBA was found in the aqueous extract. The MeOH extract of the resin was subjected to column chromatography to get various sub-fractions at different polarity of organic solvents. The sub-fraction at 4% MeOH/CHCl3 (4.1% of KBA) was found to contain the highest percentage of KBA followed by another sub-fraction at 2% MeOH/CHCl3 (2.2% of KBA). The present results also indicated that KBA is only present in the gum-resin of the trunk and not in all parts of the plant. These results were further confirmed through HPLC analysis and therefore it is concluded that NIRS coupled with PLS regression is a rapid and alternate method for quantification of KBA in Boswellia sacra. It is non-destructive, rapid, sensitive and uses simple methods of sample preparation.

A simple non-perturbing cell migration assay insensitive to proliferation effects.

PubMed

Glenn, Honor L; Messner, Jacob; Meldrum, Deirdre R

2016-08-18

Migration is a fundamental cellular behavior that plays an indispensable role in development and homeostasis, but can also contribute to pathology such as cancer metastasis. Due to its relevance to many aspects of human health, the ability to accurately measure cell migration is of broad interest, and numerous approaches have been developed. One of the most commonly employed approaches, because of its simplicity and throughput, is the exclusion zone assay in which cells are allowed to migrate into an initially cell-free region. A major drawback of this assay is that it relies on simply counting cells in the exclusion zone and therefore cannot distinguish the effects of proliferation from migration. We report here a simple modification to the exclusion zone migration assay that exclusively measures cell migration and is not affected by proliferation. This approach makes use of a lineage-tracing vital stain that is retained through cell generations and effectively reads out migration relative to the original, parental cell population. This modification is simple, robust, non-perturbing, and inexpensive. We validate the method in a panel of cell lines under conditions that inhibit or promote migration and demonstrate its use in normal and cancer cell lines as well as primary cells.

A simple non-perturbing cell migration assay insensitive to proliferation effects

PubMed Central

Glenn, Honor L.; Messner, Jacob; Meldrum, Deirdre R.

2016-01-01

Migration is a fundamental cellular behavior that plays an indispensable role in development and homeostasis, but can also contribute to pathology such as cancer metastasis. Due to its relevance to many aspects of human health, the ability to accurately measure cell migration is of broad interest, and numerous approaches have been developed. One of the most commonly employed approaches, because of its simplicity and throughput, is the exclusion zone assay in which cells are allowed to migrate into an initially cell-free region. A major drawback of this assay is that it relies on simply counting cells in the exclusion zone and therefore cannot distinguish the effects of proliferation from migration. We report here a simple modification to the exclusion zone migration assay that exclusively measures cell migration and is not affected by proliferation. This approach makes use of a lineage-tracing vital stain that is retained through cell generations and effectively reads out migration relative to the original, parental cell population. This modification is simple, robust, non-perturbing, and inexpensive. We validate the method in a panel of cell lines under conditions that inhibit or promote migration and demonstrate its use in normal and cancer cell lines as well as primary cells. PMID:27535324

NON-DESTRUCTIVE METHOD AND MEANS FOR FLAW DETECTION

DOEpatents

Hochschild, R.

1959-03-10

BS>An improved method is presented for the nondestructive detection of flaws in olectrictilly conductivc articles using magnetic field. According to thc method a homogoneous mignetic field is established in the test article;it right angle" to the artyicle. A probe is aligned with its axis transverse to the translates so hat th4 probe scans the surface of the test article while the axis of the robe is transverse to the direction of translation of the article. In this manner any output current obtained in thc probe is an indication of the size and location of a flaw in the article under test, with a miiiimum of signal pick- up in the probe from the established magnetic field.

Non-destructive vacuum decay method for pre-filled syringe closure integrity testing compared with dye ingress testing and high-voltage leak detection.

PubMed

Simonetti, Andrea; Amari, Filippo

2015-01-01

solution, preventing possible prefilled syringe plunger movement during container closure integrity testing execution, is presented as well. The growing need to meet sterile drug products' regulatory, quality, and safety expectations has progressively driven new developments and improvements both in container closure integrity testing methods and in the respective equipment, over the last years. Indeed, container closure integrity testing establishes the container closure system capability to provide required protection to the drug product and to demonstrate maintenance of product sterility over its shelf life. This article describes the development of four container closure integrity testing approaches for the evaluation of glass prefilled syringe closure integrity, including two destructive (pharmacopoeial and Novartis specific dye ingress test) and two non-destructive (vacuum decay and high-voltage leak detection) methods. The important finding from the validation of comparative studies was that the vacuum decay method resulted in the most effective, reliable and repeatable detection of defective samples, whether the defect was exposed to sterile water, to drug product, or to air. Complete sets of known defects were created for this purpose (5 μm, 10 μm, 20 μm certified leakages by laser drilled holes and capillary tubes). All investigations and studies were conducted at Bonfiglioli Engineering S.r.l. (Vigarano Pieve, Ferrara, Italy) and at Novartis Vaccines (Sovicille, Siena, Italy). © PDA, Inc. 2015.

Amino acids in a targeted versus a non-targeted metabolomics LC-MS/MS assay. Are the results consistent?

PubMed

Klepacki, Jacek; Klawitter, Jost; Klawitter, Jelena; Karimpour-Fard, Anis; Thurman, Joshua; Ingle, Gordon; Patel, Dharmesh; Christians, Uwe

2016-09-01

The results of plasma amino acid patterns in samples from kidney transplant patients with good and impaired renal function using a targeted LC-MS/MS amino acid assay and a non-targeted metabolomics assay were compared. EDTA plasma samples were prospectively collected at baseline, 1, 2, 4 and 6months post-transplant (n=116 patients, n=398 samples). Each sample was analyzed using both a commercial amino acid LC-MS/MS assay and a non-targeted metabolomics assay also based on MS/MS ion transitions. The results of both assays were independently statistically analyzed to identify amino acids associated with estimated glomerular filtration rates using correlation and partial least squares-discriminant analysis. Although there was overlap between the results of the targeted and non-targeted metabolomics assays (tryptophan, 1-methyl histidine), there were also substantial inconsistencies, with the non-targeted assay resulting in more "hits" than the targeted assay. Without further verification of the hits detected by the non-targeted discovery assay, this would have led to different interpretation of the results. There were also false negative results when the non-targeted assay was used (hydroxy proline). Several of said discrepancies could be explained by loss of sensitivity during analytical runs for selected amino acids (serine and threonine), retention time shifts, signals above the range of linear detector response and integration of peaks not separated from background and interferences (aspartate) when the non-targeted metabolomics assay was used. Whenever assessment of a specific pathway such as amino acids is the focus of interest, a targeted seems preferable to a non-targeted metabolomics assay. Copyright © 2016. Published by Elsevier Inc.

Dynamic laser speckle for non-destructive quality evaluation of bread

NASA Astrophysics Data System (ADS)

Stoykova, E.; Ivanov, B.; Shopova, M.; Lyubenova, T.; Panchev, I.; Sainov, V.

2010-10-01

Coherent illumination of a diffuse object yields a randomly varying interference pattern, which changes over time at any modification of the object. This phenomenon can be used for detection and visualization of physical or biological activity in various objects (e.g. fruits, seeds, coatings) through statistical description of laser speckle dynamics. The present report aims at non-destructive full-field evaluation of bread by spatial-temporal characterization of laser speckle. The main purpose of the conducted experiments was to prove the ability of the dynamic speckle method to indicate activity within the studied bread samples. In the set-up for acquisition and storage of dynamic speckle patterns an expanded beam from a DPSS laser (532 nm and 100mW) illuminated the sample through a ground glass diffuser. A CCD camera, adjusted to focus the sample, recorded regularly a sequence of images (8 bits and 780 x 582 squared pixels, sized 8.1 × 8.1 μm) at sampling frequency 0.25 Hz. A temporal structure function was calculated to evaluate activity of the bread samples in time using the full images in the sequence. In total, 7 samples of two types of bread were monitored during a chemical and physical process of bread's staling. Segmentation of images into matrixes of isometric fragments was also utilized. The results proved the potential of dynamic speckle as effective means for monitoring the process of bread staling and ability of this approach to differentiate between different types of bread.

Cryogenic Storage Tank Non-Destructive Evaluation

NASA Technical Reports Server (NTRS)

Arens, Ellen

2010-01-01

This slide presentation reviews the work in non-destructive evaluation (NDE) of cryogenic storage tanks. Four large cryogenic tanks, constructed in 1965 with perlite insulation in the annular regions, are of concern. The construction of the tanks, two Liquid Oxygen (LOX) and two Liquid Hydrogen (LH2), are described. The loss rate for the LOX tank at Pad A is slightly higher than that for the one at Pad B. The concerns for the LH2 tank at Pad B are that there is a significantly higher boil-off rate than that at Pad A, that there is mold growth, indicative of increased heat flow, that there is a long down-time needed for repairs, and that 3 of 5 full thermal cycles have been used on the Pad B LH2 tank. The advantages and disadvantages of thermal imaging are given. A detailed description of what is visible of the structures in the infra-red is given and views of the thermal images are included. Missing Perlite is given as the probable cause of the cold spot on the Pad B LH2 tank. There is no indications of problematic cold regions on the Pad A LH2 tank, as shown by the thermal images given in the presentation. There is definite indication of a cold region on the Pad A LOX tank. There is however concerns with thermal imaging, as thermal images can be significantly effected by environmental conditions, image differences on similar days but with different wind speeds. Other effects that must be considered include ambient temperature, humidity levels/dew, and cloud reflections

Means and method for the destruction of particles entrained in a gas stream

DOEpatents

Botts, Thomas E.; Powell, James R.

1980-01-01

An apparatus and method for the destruction of particles entrained in a gas stream are disclosed. Destruction in the context of the subject invention means the fragmentation and/or vaporization of particles above a certain size limit. The subject invention contemplates destroying such particles by exposing them to intense bursts of laser light, such light having a frequency approximately equal to or less than the mean size of such particles. This invention is particularly adopted to the protection of turbine blades in open cycle coal-fired turbine systems. Means for introducing various chemical species and activating them by exposure to laser light are also disclosed.

S.S. Annunziata Church (L'Aquila, Italy) unveiled by non- and micro-destructive testing techniques

NASA Astrophysics Data System (ADS)

Sfarra, Stefano; Cheilakou, Eleni; Theodorakeas, Panagiotis; Paoletti, Domenica; Koui, Maria

2017-03-01

The present research work explores the potential of an integrated inspection methodology, combining Non-destructive testing and micro-destructive analytical techniques, for both the structural assessment of the S.S. Annunziata Church located in Roio Colle (L'Aquila, Italy) and the characterization of its wall paintings' pigments. The study started by applying passive thermal imaging for the structural monitoring of the church before and after the application of a consolidation treatment, while active thermal imaging was further used for assessing this consolidation procedure. After the earthquake of 2009, which seriously damaged the city of L'Aquila and its surroundings, part of the internal plaster fell off revealing the presence of an ancient mural painting that was subsequently investigated by means of a combined analytical approach involving portable VIS-NIR fiber optics diffuse reflectance spectroscopy (FORS) and laboratory methods, such as environmental scanning electron microscopy (ESEM) coupled with energy dispersive X-ray analysis (EDX), and attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR). The results obtained from the thermographic analysis provided information concerning the two different constrictive phases of the Church, enabled the assessment of the consolidation treatment, and contributed to the detection of localized problems mainly related to the rising damp phenomenon and to biological attack. In addition, the results obtained from the combined analytical approach allowed the identification of the wall painting pigments (red and yellow ochre, green earth, and smalt) and provided information on the binding media and the painting technique possibly applied by the artist. From the results of the present study, it is possible to conclude that the joint use of the above stated methods into an integrated methodology can produce the complete set of useful information required for the planning of the Church's restoration

Application of bacterial reverse mutation assay for detection of non-genotoxic carcinogens.

PubMed

Kanode, Rewan; Chandra, Saurabh; Sharma, Sharad

2017-06-01

Non-genotoxic carcinogens may play a significant role in development of cancer. Currently short-term assays for mutagenicity classify genotoxic carcinogens and lack the abilities to detect epigenetic carcinogens. The need to develop an endpoint always remains to recognize potentially carcinogenic agents employing rapid and practical bioassays. For this, the present study utilized TA98 and TA1537 tester strains of Salmonella typhimurium to evaluate four non-genotoxic carcinogenic agents (Coumarin, β-Myrcene, Bis(2-ethylhexyl) phthalate and trans-anethole). These chemicals were tested individually and in combination with promutagens 2-aminoanthracene (2AA) and benzo(a)pyrene (BP) in presence of metabolic activation system (S9) by plate incorporation method. Exposure to all four test chemicals revealed marked increase of revertant colonies in promutagen combined groups as compared to promutagens alone. However significantly greater fold responses were observed with 2AA combination groups (Coumarin +2AA, β-Myrcene +2AA, Bis(2-ethylhexyl) phthalate +2AA and trans-anethole +2AA) with TA98 strain as compared with TA1537, which seems to have enhanced the mutagenic response of 2AA in metabolically activated conditions. It is concluded that out of both tester strains TA98 strain of Salmonella typhimurium has the potential to detect non-genotoxic carcinogens when combined with potent promutgens either by inhibiting or modulating activities of liver microsomal enzymes biochemically which may indirectly contribute to neoplastic alterations. Further this simple, short-term alternative assay may provide rapid information during extrapolative toxicology for differentiating genotoxic and non-genotoxic carcinogens.

Multispectral UV imaging for fast and non-destructive quality control of chemical and physical tablet attributes.

PubMed

Klukkert, Marten; Wu, Jian X; Rantanen, Jukka; Carstensen, Jens M; Rades, Thomas; Leopold, Claudia S

2016-07-30

Monitoring of tablet quality attributes in direct vicinity of the production process requires analytical techniques that allow fast, non-destructive, and accurate tablet characterization. The overall objective of this study was to investigate the applicability of multispectral UV imaging as a reliable, rapid technique for estimation of the tablet API content and tablet hardness, as well as determination of tablet intactness and the tablet surface density profile. One of the aims was to establish an image analysis approach based on multivariate image analysis and pattern recognition to evaluate the potential of UV imaging for automatized quality control of tablets with respect to their intactness and surface density profile. Various tablets of different composition and different quality regarding their API content, radial tensile strength, intactness, and surface density profile were prepared using an eccentric as well as a rotary tablet press at compression pressures from 20MPa up to 410MPa. It was found, that UV imaging can provide both, relevant information on chemical and physical tablet attributes. The tablet API content and radial tensile strength could be estimated by UV imaging combined with partial least squares analysis. Furthermore, an image analysis routine was developed and successfully applied to the UV images that provided qualitative information on physical tablet surface properties such as intactness and surface density profiles, as well as quantitative information on variations in the surface density. In conclusion, this study demonstrates that UV imaging combined with image analysis is an effective and non-destructive method to determine chemical and physical quality attributes of tablets and is a promising approach for (near) real-time monitoring of the tablet compaction process and formulation optimization purposes. Copyright © 2015 Elsevier B.V. All rights reserved.

Non-destructive state detection for quantum logic spectroscopy of molecular ions.

PubMed

Wolf, Fabian; Wan, Yong; Heip, Jan C; Gebert, Florian; Shi, Chunyan; Schmidt, Piet O

2016-02-25

Precision laser spectroscopy of cold and trapped molecular ions is a powerful tool in fundamental physics--used, for example, in determining fundamental constants, testing for their possible variation in the laboratory, and searching for a possible electric dipole moment of the electron. However, the absence of cycling transitions in molecules poses a challenge for direct laser cooling of the ions, and for controlling and detecting their quantum states. Previously used state-detection techniques based on photodissociation or chemical reactions are destructive and therefore inefficient, restricting the achievable resolution in laser spectroscopy. Here, we experimentally demonstrate non-destructive detection of the quantum state of a single trapped molecular ion through its strong Coulomb coupling to a well controlled, co-trapped atomic ion. An algorithm based on a state-dependent optical dipole force changes the internal state of the atom according to the internal state of the molecule. We show that individual quantum states in the molecular ion can be distinguished by the strength of their coupling to the optical dipole force. We also observe quantum jumps (induced by black-body radiation) between rotational states of a single molecular ion. Using the detuning dependence of the state-detection signal, we implement a variant of quantum logic spectroscopy of a molecular resonance. Our state-detection technique is relevant to a wide range of molecular ions, and could be applied to state-controlled quantum chemistry and to spectroscopic investigations of molecules that serve as probes for interstellar clouds.

THz QCL-based active imaging dedicated to non-destructive testing of composite materials used in aeronautics

NASA Astrophysics Data System (ADS)

Destic, F.; Petitjean, Y.; Massenot, S.; Mollier, J.-C.; Barbieri, S.

2010-08-01

This paper presents a CW raster-scanning THz imaging setup, used to perform Non-Destructive Testing of KevlarTMand carbon fibre samples. The setup uses a 2.5 THz Quantum Cascade Laser as a source. Delamination defect in a Kevlar sample was detected showing a sensitivity to laser polarization orientation. Detection of a break in a carbon/epoxy sample was also performed.

A generalized method for characterization of 235U and 239Pu content using short-lived fission product gamma spectroscopy

DOE PAGES

Knowles, Justin R.; Skutnik, Steven E.; Glasgow, David C.; ...

2016-06-23

Rapid non-destructive assay methods for trace fissile material analysis are needed in both nuclear forensics and safeguards communities. To address these needs, research at the High Flux Isotope Reactor Neutron Activation Analysis laboratory has developed a generalized non-destructive assay method to characterize materials containing fissile isotopes. This method relies on gamma-ray emissions from short-lived fission products and capitalizes off of differences in fission product yields to identify fissile compositions of trace material samples. Although prior work has explored the use of short-lived fission product gamma-ray measurements, the proposed method is the first to provide a holistic characterization of isotopic identification,more » mass ratios, and absolute mass determination. Successful single fissile isotope mass recoveries of less than 6% error have been conducted on standards of 235U and 239Pu as low as 12 nanograms in less than 10 minutes. Additionally, mixtures of fissile isotope standards containing 235U and 239Pu have been characterized as low as 229 nanograms of fissile mass with less than 12% error. The generalizability of this method is illustrated by evaluating different fissile isotopes, mixtures of fissile isotopes, and two different irradiation positions in the reactor. Furthermore, it is anticipated that this method will be expanded to characterize additional fissile nuclides, utilize various irradiation sources, and account for increasingly complex sample matrices.« less

A generalized method for characterization of 235U and 239Pu content using short-lived fission product gamma spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knowles, Justin R.; Skutnik, Steven E.; Glasgow, David C.

Rapid non-destructive assay methods for trace fissile material analysis are needed in both nuclear forensics and safeguards communities. To address these needs, research at the High Flux Isotope Reactor Neutron Activation Analysis laboratory has developed a generalized non-destructive assay method to characterize materials containing fissile isotopes. This method relies on gamma-ray emissions from short-lived fission products and capitalizes off of differences in fission product yields to identify fissile compositions of trace material samples. Although prior work has explored the use of short-lived fission product gamma-ray measurements, the proposed method is the first to provide a holistic characterization of isotopic identification,more » mass ratios, and absolute mass determination. Successful single fissile isotope mass recoveries of less than 6% error have been conducted on standards of 235U and 239Pu as low as 12 nanograms in less than 10 minutes. Additionally, mixtures of fissile isotope standards containing 235U and 239Pu have been characterized as low as 229 nanograms of fissile mass with less than 12% error. The generalizability of this method is illustrated by evaluating different fissile isotopes, mixtures of fissile isotopes, and two different irradiation positions in the reactor. Furthermore, it is anticipated that this method will be expanded to characterize additional fissile nuclides, utilize various irradiation sources, and account for increasingly complex sample matrices.« less

Variation in gymnemic acid content and non-destructive harvesting of Gymnema sylvestre (Gudmar).

PubMed

Pandey, Ashok Kumar; Yadav, Swati

2010-09-01

Madhunashini (Gymnema sylvestre R. Br.) commonly known as 'Gudmar' in Hindi is an important medicinal climber and extensively used in almost all Indian System of Medicine as a remedy for diabetes, rheumatism, cough, ulcer, jaundice, dyspepsia, constipation, eyes pain and also in snakebite. In India, it is found growing in Andhra Pradesh, Bihar, Chhattisgarh, Karnataka, Kerala, Madhya Pradesh, Maharastra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal. The major phytoconstituents are gymnemic acids, gudmarin and saponins. In the present study, Gymnema germplasm collected from various regions of Madhya Pradesh was evaluated on the basis of their morphological characteristics and gymnemic acid content. Gymnemic acid content in the leaves was estimated by HPLC. We have also standardized the non-destructive harvesting practices of Gudmar. Selective harvesting was done without harming the main plant. Only mature leaves (60%) were hand plucked in the month of October. Second harvest was done in the month of June. Data revealed that gymnemic acid content varied between 0.96% ± 0.03 (Seoni) to 1.58% ±0.03 (Amarkantak). It was also observed that the leaves left at the time of 1(st) harvest during October matured in June at the time of 2(nd) harvest. Non destructive harvesting practice did not have any negative impact on overall development of the plant. It is evident that there is wide variation in the morphological characteristics and gymnemic acid content in G. sylvestre collected from various locations, which can be exploited for further crop improvement programmes.

Systems, devices, and methods for agglutination assays using sedimentation

DOEpatents

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

2016-01-26

Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.

The Impact of Gate Width Setting and Gate Utilization Factors on Plutonium Assay in Passive Correlated Neutron Counting

DOE PAGES

Henzlova, Daniela; Menlove, Howard Olsen; Croft, Stephen; ...

2015-06-15

In the field of nuclear safeguards, passive neutron multiplicity counting (PNMC) is a method typically employed in non-destructive assay (NDA) of special nuclear material (SNM) for nonproliferation, verification and accountability purposes. PNMC is generally performed using a well-type thermal neutron counter and relies on the detection of correlated pairs or higher order multiplets of neutrons emitted by an assayed item. To assay SNM, a set of parameters for a given well-counter is required to link the measured multiplicity rates to the assayed item properties. Detection efficiency, die-away time, gate utilization factors (tightly connected to die-away time) as well as optimummore » gate width setting are among the key parameters. These parameters along with the underlying model assumptions directly affect the accuracy of the SNM assay. In this paper we examine the role of gate utilization factors and the single exponential die-away time assumption and their impact on the measurements for a range of plutonium materials. In addition, we examine the importance of item-optimized coincidence gate width setting as opposed to using a universal gate width value. Finally, the traditional PNMC based on multiplicity shift register electronics is extended to Feynman-type analysis and application of this approach to Pu mass assay is demonstrated.« less

Investigation and development of a non-destructive system to evaluate critical properties of asphalt pavements during the compaction process.

DOT National Transportation Integrated Search

2013-10-01

The purpose of this report is to present findings from a two-stage investigation to develop a non-destructive system to : evaluate critical properties and characteristics of asphalt pavements during the compaction process. The first stage aligned : c...

A non-isotopic assay uses bromouridine and RNA synthesis to detect DNA damage responses.

PubMed

Hasegawa, Mayu; Iwai, Shigenori; Kuraoka, Isao

2010-06-17

Individuals with inherited xeroderma pigmentosum (XP) disorder and Cockayne syndrome (CS) are deficient in nucleotide excision repair and experience hypersensitivity to sunlight. Although there are several diagnostic assays for these disorders, the recovery of RNA synthesis (RRS) assay that can discriminate between XP cells and CS cells is very laborious. Here, we report on a novel non-radioisotope RRS assay that uses bromouridine (a uridine analog) as an alternative to (3)H-uridine. This assay can easily detect RNA polymerase I transcription in nucleoli and RNA polymerase II transcription in nuclei. The non-RI RSS assay also can rapidly detect normal RRS activity in HeLa cells. Thus, this assay is useful as a novel and easy technique for CS diagnosis. Because RRS is thought to be related to transcription-coupled DNA repair, which is triggered by the blockage of transcriptional machinery by DNA lesions, this assay may be of use for analysis of DNA repair, transcription, and/or genetic toxicity. Copyright 2010 Elsevier B.V. All rights reserved.

An x ray scatter approach for non-destructive chemical analysis of low atomic numbered elements

NASA Technical Reports Server (NTRS)

Ross, H. Richard

1993-01-01

A non-destructive x-ray scatter (XRS) approach has been developed, along with a rapid atomic scatter algorithm for the detection and analysis of low atomic-numbered elements in solids, powders, and liquids. The present method of energy dispersive x-ray fluorescence spectroscopy (EDXRF) makes the analysis of light elements (i.e., less than sodium; less than 11) extremely difficult. Detection and measurement become progressively worse as atomic numbers become smaller, due to a competing process called 'Auger Emission', which reduces fluorescent intensity, coupled with the high mass absorption coefficients exhibited by low energy x-rays, the detection and determination of low atomic-numbered elements by x-ray spectrometry is limited. However, an indirect approach based on the intensity ratio of Compton and Rayleigh scattered has been used to define light element components in alloys, plastics and other materials. This XRS technique provides qualitative and quantitative information about the overall constituents of a variety of samples.

Recent Advances in Active Infrared Thermography for Non-Destructive Testing of Aerospace Components.

PubMed

Ciampa, Francesco; Mahmoodi, Pooya; Pinto, Fulvio; Meo, Michele

2018-02-16

Active infrared thermography is a fast and accurate non-destructive evaluation technique that is of particular relevance to the aerospace industry for the inspection of aircraft and helicopters' primary and secondary structures, aero-engine parts, spacecraft components and its subsystems. This review provides an exhaustive summary of most recent active thermographic methods used for aerospace applications according to their physical principle and thermal excitation sources. Besides traditional optically stimulated thermography, which uses external optical radiation such as flashes, heaters and laser systems, novel hybrid thermographic techniques are also investigated. These include ultrasonic stimulated thermography, which uses ultrasonic waves and the local damage resonance effect to enhance the reliability and sensitivity to micro-cracks, eddy current stimulated thermography, which uses cost-effective eddy current excitation to generate induction heating, and microwave thermography, which uses electromagnetic radiation at the microwave frequency bands to provide rapid detection of cracks and delamination. All these techniques are here analysed and numerous examples are provided for different damage scenarios and aerospace components in order to identify the strength and limitations of each thermographic technique. Moreover, alternative strategies to current external thermal excitation sources, here named as material-based thermography methods, are examined in this paper. These novel thermographic techniques rely on thermoresistive internal heating and offer a fast, low power, accurate and reliable assessment of damage in aerospace composites.

Recent Advances in Active Infrared Thermography for Non-Destructive Testing of Aerospace Components

PubMed Central

Mahmoodi, Pooya; Pinto, Fulvio; Meo, Michele

2018-01-01

Active infrared thermography is a fast and accurate non-destructive evaluation technique that is of particular relevance to the aerospace industry for the inspection of aircraft and helicopters’ primary and secondary structures, aero-engine parts, spacecraft components and its subsystems. This review provides an exhaustive summary of most recent active thermographic methods used for aerospace applications according to their physical principle and thermal excitation sources. Besides traditional optically stimulated thermography, which uses external optical radiation such as flashes, heaters and laser systems, novel hybrid thermographic techniques are also investigated. These include ultrasonic stimulated thermography, which uses ultrasonic waves and the local damage resonance effect to enhance the reliability and sensitivity to micro-cracks, eddy current stimulated thermography, which uses cost-effective eddy current excitation to generate induction heating, and microwave thermography, which uses electromagnetic radiation at the microwave frequency bands to provide rapid detection of cracks and delamination. All these techniques are here analysed and numerous examples are provided for different damage scenarios and aerospace components in order to identify the strength and limitations of each thermographic technique. Moreover, alternative strategies to current external thermal excitation sources, here named as material-based thermography methods, are examined in this paper. These novel thermographic techniques rely on thermoresistive internal heating and offer a fast, low power, accurate and reliable assessment of damage in aerospace composites. PMID:29462953

Evaluation of methods for the assay of radium-228 in water

DOE Office of Scientific and Technical Information (OSTI.GOV)

Noyce, J.R.

1981-02-01

The technical literature from 1967 to May 1980 was searched for methods for assaying radium-228 in water. These methods were evaluated for their suitability as potential EPA reference methods for drinking water assays. The authors suggest the present EPA reference method (Krieger, 1976) be retained but improved, and a second method (McCurdy and Mellor, 1979), which employs beta-gamma coincidence counting, be added. Included in this report is a table that lists the principal features of 17 methods for radium-228 assays.

Crime scene investigations using portable, non-destructive space exploration technology

NASA Technical Reports Server (NTRS)

Trombka, Jacob I.; Schweitzer, Jeffrey; Selavka, Carl; Dale, Mark; Gahn, Norman; Floyd, Samuel; Marie, James; Hobson, Maritza; Zeosky, Jerry; Martin, Ken;

Non-destructive inspection protocol for reinforced concrete barriers and bridge railings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chintakunta, Satish R.; Boone, Shane D.

Reinforced concrete highway barriers and bridge railings serve to prevent errant vehicles from departing the travel way at grade separations. Despite the important role that they play in maintaining safety and their ubiquitous nature, barrier inspection rarely moves beyond visual inspection. In August 2008, a tractor-trailer fatally departed William Preston Lane, Jr. Memorial Bridge after it dislodged a section of the bridge barrier. Investigations following the accident identified significant corrosion of the anchor bolts attaching the bridge railing to the bridge deck. As a result of the information gathered during its investigation of the accident, the National Transportation Safety Boardmore » (NTSB) made recommendations to the Federal Highway Administration concerning Non-Destructive Evaluation (NDE) of concrete bridge railings. The Center for nondestructive evaluation (NDE) at Turner Fairbank Highway Research Center in McLean, VA is currently evaluating feasibility of using four technologies - ground penetrating radar (GPR), ultrasonic pulse-echo, digital radiography and infrared thermal imaging methods to develop bridge inspection methods that augment visual inspections, offer reliable measurement techniques, and are practical, both in terms of time and cost, for field inspection work. Controlled samples containing predefined corrosion levels in reinforcing steel were embedded at barrier connection points for laboratory testing. All four NDE techniques were used in the initial phase I testing. An inspection protocol for detecting and measuring the corrosion of reinforced steel embedded in the anchorage system will be developed as part of phase II research. The identified technologies shall be further developed for field testing utilizing a structure with a barrier in good condition and a structure with a barrier in poor condition.« less

Non-destructive inspection protocol for reinforced concrete barriers and bridge railings

NASA Astrophysics Data System (ADS)

Chintakunta, Satish R.; Boone, Shane D.

2014-02-01

Reinforced concrete highway barriers and bridge railings serve to prevent errant vehicles from departing the travel way at grade separations. Despite the important role that they play in maintaining safety and their ubiquitous nature, barrier inspection rarely moves beyond visual inspection. In August 2008, a tractor-trailer fatally departed William Preston Lane, Jr. Memorial Bridge after it dislodged a section of the bridge barrier. Investigations following the accident identified significant corrosion of the anchor bolts attaching the bridge railing to the bridge deck. As a result of the information gathered during its investigation of the accident, the National Transportation Safety Board (NTSB) made recommendations to the Federal Highway Administration concerning Non-Destructive Evaluation (NDE) of concrete bridge railings. The Center for nondestructive evaluation (NDE) at Turner Fairbank Highway Research Center in McLean, VA is currently evaluating feasibility of using four technologies - ground penetrating radar (GPR), ultrasonic pulse-echo, digital radiography and infrared thermal imaging methods to develop bridge inspection methods that augment visual inspections, offer reliable measurement techniques, and are practical, both in terms of time and cost, for field inspection work. Controlled samples containing predefined corrosion levels in reinforcing steel were embedded at barrier connection points for laboratory testing. All four NDE techniques were used in the initial phase I testing. An inspection protocol for detecting and measuring the corrosion of reinforced steel embedded in the anchorage system will be developed as part of phase II research. The identified technologies shall be further developed for field testing utilizing a structure with a barrier in good condition and a structure with a barrier in poor condition.

Fractal dimension analysis for robust ultrasonic non-destructive evaluation (NDE) of coarse grained materials

NASA Astrophysics Data System (ADS)

Li, Minghui; Hayward, Gordon

2018-04-01

Over the recent decades, there has been a growing demand on reliable and robust non-destructive evaluation (NDE) of structures and components made from coarse grained materials such as alloys, stainless steels, carbon-reinforced composites and concrete; however, when inspected using ultrasound, the flaw echoes are usually contaminated by high-level, time-invariant, and correlated grain noise originating from the microstructure and grain boundaries, leading to pretty low signal-to-noise ratio (SNR) and the flaw information being obscured or completely hidden by the grain noise. In this paper, the fractal dimension analysis of the A-scan echoes is investigated as a measure of complexity of the time series to distinguish the echoes originating from the real defects and the grain noise, and then the normalized fractal dimension coefficients are applied to the amplitudes as the weighting factor to enhance the SNR and defect detection. Experiments on industrial samples of the mild steel and the stainless steel are conducted and the results confirm the great benefits of the method.

Applications Where Snap is BPM for Radioactive Waste Assay

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, T.J.

2008-07-01

Historically, the Atomic Weapons Establishment (AWE) at Aldermaston in the United Kingdom (UK), has used a variety of assay techniques to measure the radioactive content of a diverse range of waste packages from decommissioning, operational and legacy sources. The regulator, the Environment Agency in the UK, places conditions and limits on AWE through an authorisation within the Radioactive Substances Act (RSA93). The conditions and limits require Best Practical Means (BPM) measurements to be used to demonstrate compliance with the authorisation. Hence, the assay technique employed needs to achieve a balance between risk of exposure, environmental considerations, technological considerations, health andmore » safety considerations and cost effectiveness, without being grossly disproportionate in terms of money, time or trouble. Recently published work has concluded that the Spectral Non-destructive Assay Platform (SNAP) assay system is BPM for Depleted Uranium (DU) waste assay at AWE (1) and low level plutonium in soft drummed waste, HEPA filters and soils (2-4). The purpose of this paper is to highlight other applications where SNAP represents BPM for radioactive waste assay. This has been done by intercomparison studies of SNAP with other assay techniques, such as Segmented Gamma Scanner (SGS) and Passive Neutron Coincidence Counter (PNCC). It has been concluded that, for a large range of waste packages encountered at AWE, SNAP is BPM. (author)« less

Three-dimensional non-destructive optical evaluation of laser-processing performance using optical coherence tomography.

PubMed

Kim, Youngseop; Choi, Eun Seo; Kwak, Wooseop; Shin, Yongjin; Jung, Woonggyu; Ahn, Yeh-Chan; Chen, Zhongping

2008-06-01

We demonstrate the use of optical coherence tomography (OCT) as a non-destructive diagnostic tool for evaluating laser-processing performance by imaging the features of a pit and a rim. A pit formed on a material at different laser-processing conditions is imaged using both a conventional scanning electron microscope (SEM) and OCT. Then using corresponding images, the geometrical characteristics of the pit are analyzed and compared. From the results, we could verify the feasibility and the potential of the application of OCT to the monitoring of the laser-processing performance.

Three-dimensional non-destructive optical evaluation of laser-processing performance using optical coherence tomography

PubMed Central

Kim, Youngseop; Choi, Eun Seo; Kwak, Wooseop; Shin, Yongjin; Jung, Woonggyu; Ahn, Yeh-Chan; Chen, Zhongping

2014-01-01

We demonstrate the use of optical coherence tomography (OCT) as a non-destructive diagnostic tool for evaluating laser-processing performance by imaging the features of a pit and a rim. A pit formed on a material at different laser-processing conditions is imaged using both a conventional scanning electron microscope (SEM) and OCT. Then using corresponding images, the geometrical characteristics of the pit are analyzed and compared. From the results, we could verify the feasibility and the potential of the application of OCT to the monitoring of the laser-processing performance. PMID:24932051

Infrared thermography non-destructive evaluation of lithium-ion battery

NASA Astrophysics Data System (ADS)

Wang, Zi-jun; Li, Zhi-qiang; Liu, Qiang

2011-08-01

The power lithium-ion battery with its high specific energy, high theoretical capacity and good cycle-life is a prime candidate as a power source for electric vehicles (EVs) and hybrid electric vehicles (HEVs). Safety is especially important for large-scale lithium-ion batteries, especially the thermal analysis is essential for their development and design. Thermal modeling is an effective way to understand the thermal behavior of the lithium-ion battery during charging and discharging. With the charging and discharging, the internal heat generation of the lithium-ion battery becomes large, and the temperature rises leading to an uneven temperature distribution induces partial degradation. Infrared (IR) Non-destructive Evaluation (NDE) has been well developed for decades years in materials, structures, and aircraft. Most thermographic methods need thermal excitation to the measurement structures. In NDE of battery, the thermal excitation is the heat generated from carbon and cobalt electrodes in electrolyte. A technique named "power function" has been developed to determine the heat by chemical reactions. In this paper, the simulations of the transient response of the temperature distribution in the lithium-ion battery are developed. The key to resolving the security problem lies in the thermal controlling, including the heat generation and the internal and external heat transfer. Therefore, three-dimensional modelling for capturing geometrical thermal effects on battery thermal abuse behaviour is required. The simulation model contains the heat generation during electrolyte decomposition and electrical resistance component. Oven tests are simulated by three-dimensional model and the discharge test preformed by test system. Infrared thermography of discharge is recorded in order to analyze the security of the lithium-ion power battery. Nondestructive detection is performed for thermal abuse analysis and discharge analysis.

A Flow Cytometry-Based Assay for Quantifying Non-Plaque Forming Strains of Yellow Fever Virus

PubMed Central

Hammarlund, Erika; Amanna, Ian J.; Dubois, Melissa E.; Barron, Alex; Engelmann, Flora; Messaoudi, Ilhem; Slifka, Mark K.

2012-01-01

Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE) on cell monolayers. In our hands, the Dakar strain of yellow fever virus (YFV-Dakar) could not be measured by plaque assay (PA), focus-forming assay (FFA), or by measurement of CPE. For these reasons, we developed a YFV-specific monoclonal antibody (3A8.B6) and used it to optimize a highly sensitive flow cytometry-based tissue culture limiting dilution assay (TC-LDA) to measure levels of infectious virus. The TC-LDA was performed by incubating serial dilutions of virus in replicate wells of C6/36 cells and stained intracellularly for virus with MAb 3A8.B6. Using this approach, we could reproducibly quantitate YFV-Dakar in tissue culture supernatants as well as from the serum of viremic rhesus macaques experimentally infected with YFV-Dakar. Moreover, the TC-LDA approach was >10-fold more sensitive than standard plaque assay for quantitating typical plaque-forming strains of YFV including YFV-17D and YFV-FNV (French neurotropic vaccine). Together, these results indicate that the TC-LDA technique is effective for quantitating both plaque-forming and non-plaque-forming strains of yellow fever virus, and this methodology may be readily adapted for the study and quantitation of other non-plaque-forming viruses. PMID:23028428

A flow cytometry-based assay for quantifying non-plaque forming strains of yellow fever virus.

PubMed

Hammarlund, Erika; Amanna, Ian J; Dubois, Melissa E; Barron, Alex; Engelmann, Flora; Messaoudi, Ilhem; Slifka, Mark K

2012-01-01

Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE) on cell monolayers. In our hands, the Dakar strain of yellow fever virus (YFV-Dakar) could not be measured by plaque assay (PA), focus-forming assay (FFA), or by measurement of CPE. For these reasons, we developed a YFV-specific monoclonal antibody (3A8.B6) and used it to optimize a highly sensitive flow cytometry-based tissue culture limiting dilution assay (TC-LDA) to measure levels of infectious virus. The TC-LDA was performed by incubating serial dilutions of virus in replicate wells of C6/36 cells and stained intracellularly for virus with MAb 3A8.B6. Using this approach, we could reproducibly quantitate YFV-Dakar in tissue culture supernatants as well as from the serum of viremic rhesus macaques experimentally infected with YFV-Dakar. Moreover, the TC-LDA approach was >10-fold more sensitive than standard plaque assay for quantitating typical plaque-forming strains of YFV including YFV-17D and YFV-FNV (French neurotropic vaccine). Together, these results indicate that the TC-LDA technique is effective for quantitating both plaque-forming and non-plaque-forming strains of yellow fever virus, and this methodology may be readily adapted for the study and quantitation of other non-plaque-forming viruses.

Failure analysis of electronic parts: Laboratory methods. [for destructive and nondestructive testing

NASA Technical Reports Server (NTRS)

Anstead, R. J. (Editor); Goldberg, E. (Editor)

1975-01-01

Failure analysis test methods are presented for use in analyzing candidate electronic parts and in improving future design reliability. Each test is classified as nondestructive, semidestructive, or destructive. The effects upon applicable part types (i.e. integrated circuit, transitor) are discussed. Methodology is given for performing the following: immersion tests, radio graphic tests, dewpoint tests, gas ambient analysis, cross sectioning, and ultraviolet examination.

A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe.

PubMed

Su, Pai-Hsiang; Lai, Yen-Hsun

2017-01-01

required to collapse the ΔpH env was then studied. The establishment of a non-destructive method of monitoring the stromal pH will be valuable for studying the roles of the ΔpH env in chloroplast physiology.

Semi-Tomographic Gamma Scanning Technique for Non-Destructive Assay of Radioactive Waste Drums

NASA Astrophysics Data System (ADS)

Gu, Weiguo; Rao, Kaiyuan; Wang, Dezhong; Xiong, Jiemei

2016-12-01

Segmented gamma scanning (SGS) and tomographic gamma scanning (TGS) are two traditional detection techniques for low and intermediate level radioactive waste drum. This paper proposes one detection method named semi-tomographic gamma scanning (STGS) to avoid the poor detection accuracy of SGS and shorten detection time of TGS. This method and its algorithm synthesize the principles of SGS and TGS. In this method, each segment is divided into annual voxels and tomography is used in the radiation reconstruction. The accuracy of STGS is verified by experiments and simulations simultaneously for the 208 liter standard waste drums which contains three types of nuclides. The cases of point source or multi-point sources, uniform or nonuniform materials are employed for comparison. The results show that STGS exhibits a large improvement in the detection performance, and the reconstruction error and statistical bias are reduced by one quarter to one third or less for most cases if compared with SGS.

Non-invasive assessments of adipose tissue metabolism in vitro

PubMed Central

Abbott, Rosalyn D.; Borowsky, Francis E.; Quinn, Kyle P.; Bernstein, David L.; Georgakoudi, Irene; Kaplan, David L.

2015-01-01

Adipose tissue engineering is a diverse area of research where the developed tissues can be used to study normal adipose tissue functions, create disease models in vitro, and replace soft tissue defects in vivo. Increasing attention has been focused on the highly specialized metabolic pathways that regulate energy storage and release in adipose tissues which affect local and systemic outcomes. Non-invasive, dynamic measurement systems are useful to track these metabolic pathways in the same tissue model over time to evaluate long term cell growth, differentiation, and development within tissue engineering constructs. This approach reduces costs and time in comparison to more traditional destructive methods such as biochemical and immunochemistry assays and proteomics assessments. Towards this goal, this review will focus on important metabolic functions of adipose tissues and strategies to evaluate them with noninvasive in vitro methods. Current non-invasive methods, such as measuring key metabolic markers and endogenous contrast imaging will be explored. PMID:26399988

Non-thermal plasma destruction of allyl alcohol in waste gas: kinetics and modelling

NASA Astrophysics Data System (ADS)

DeVisscher, A.; Dewulf, J.; Van Durme, J.; Leys, C.; Morent, R.; Van Langenhove, H.

2008-02-01

Non-thermal plasma treatment is a promising technique for the destruction of volatile organic compounds in waste gas. A relatively unexplored technique is the atmospheric negative dc multi-pin-to-plate glow discharge. This paper reports experimental results of allyl alcohol degradation and ozone production in this type of plasma. A new model was developed to describe these processes quantitatively. The model contains a detailed chemical degradation scheme, and describes the physics of the plasma by assuming that the fraction of electrons that takes part in chemical reactions is an exponential function of the reduced field. The model captured the experimental kinetic data to less than 2 ppm standard deviation.

Ultra-portable, wireless smartphone spectrometer for rapid, non-destructive testing of fruit ripeness.

PubMed

Das, Anshuman J; Wahi, Akshat; Kothari, Ishan; Raskar, Ramesh

2016-09-08

We demonstrate a smartphone based spectrometer design that is standalone and supported on a wireless platform. The device is inherently low-cost and the power consumption is minimal making it portable to carry out a range of studies in the field. All essential components of the device like the light source, spectrometer, filters, microcontroller and wireless circuits have been assembled in a housing of dimensions 88 mm × 37 mm × 22 mm and the entire device weighs 48 g. The resolution of the spectrometer is 15 nm, delivering accurate and repeatable measurements. The device has a dedicated app interface on the smartphone to communicate, receive, plot and analyze spectral data. The performance of the smartphone spectrometer is comparable to existing bench-top spectrometers in terms of stability and wavelength resolution. Validations of the device were carried out by demonstrating non-destructive ripeness testing in fruit samples. Ultra-Violet (UV) fluorescence from Chlorophyll present in the skin was measured across various apple varieties during the ripening process and correlated with destructive firmness tests. A satisfactory agreement was observed between ripeness and fluorescence signals. This demonstration is a step towards possible consumer, bio-sensing and diagnostic applications that can be carried out in a rapid manner.

Ultra-portable, wireless smartphone spectrometer for rapid, non-destructive testing of fruit ripeness

NASA Astrophysics Data System (ADS)

Das, Anshuman J.; Wahi, Akshat; Kothari, Ishan; Raskar, Ramesh

2016-09-01

We demonstrate a smartphone based spectrometer design that is standalone and supported on a wireless platform. The device is inherently low-cost and the power consumption is minimal making it portable to carry out a range of studies in the field. All essential components of the device like the light source, spectrometer, filters, microcontroller and wireless circuits have been assembled in a housing of dimensions 88 mm × 37 mm × 22 mm and the entire device weighs 48 g. The resolution of the spectrometer is 15 nm, delivering accurate and repeatable measurements. The device has a dedicated app interface on the smartphone to communicate, receive, plot and analyze spectral data. The performance of the smartphone spectrometer is comparable to existing bench-top spectrometers in terms of stability and wavelength resolution. Validations of the device were carried out by demonstrating non-destructive ripeness testing in fruit samples. Ultra-Violet (UV) fluorescence from Chlorophyll present in the skin was measured across various apple varieties during the ripening process and correlated with destructive firmness tests. A satisfactory agreement was observed between ripeness and fluorescence signals. This demonstration is a step towards possible consumer, bio-sensing and diagnostic applications that can be carried out in a rapid manner.

Non-destructive ion trap mass spectrometer and method

DOEpatents

Frankevich, Vladimir E.; Soni, Manish H.; Nappi, Mario; Santini, Robert E.; Amy, Jonathan W.; Cooks, Robert G.

1997-01-01

The invention relates to an ion trap mass spectrometer of the type having an ion trapping volume defined by spaced end caps and a ring electrode. The ion trap includes a small sensing electrode which senses characteristic motion of ions trapped in said trapping volume and provides an image current. Ions are excited into characteristic motion by application of an excitation pulse to the trapped ions. The invention also relates to a method of operating such an ion trap.

Non-contact, non-destructive, quantitative probing of interfacial trap sites for charge carrier transport at semiconductor-insulator boundary

NASA Astrophysics Data System (ADS)

Choi, Wookjin; Miyakai, Tomoyo; Sakurai, Tsuneaki; Saeki, Akinori; Yokoyama, Masaaki; Seki, Shu

2014-07-01

The density of traps at semiconductor-insulator interfaces was successfully estimated using microwave dielectric loss spectroscopy with model thin-film organic field-effect transistors. The non-contact, non-destructive analysis technique is referred to as field-induced time-resolved microwave conductivity (FI-TRMC) at interfaces. Kinetic traces of FI-TRMC transients clearly distinguished the mobile charge carriers at the interfaces from the immobile charges trapped at defects, allowing both the mobility of charge carriers and the number density of trap sites to be determined at the semiconductor-insulator interfaces. The number density of defects at the interface between evaporated pentacene on a poly(methylmethacrylate) insulating layer was determined to be 1012 cm-2, and the hole mobility was up to 6.5 cm2 V-1 s-1 after filling the defects with trapped carriers. The FI-TRMC at interfaces technique has the potential to provide rapid screening for the assessment of interfacial electronic states in a variety of semiconductor devices.

Bulk Electrical Cable Non-Destructive Examination Methods for Nuclear Power Plant Cable Aging Management Programs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glass, Samuel W.; Jones, Anthony M.; Fifield, Leonard S.

This Pacific Northwest National Laboratory milestone report describes progress to date on the investigation of nondestructive test methods focusing particularly on bulk electrical test methods that provide key indicators of cable aging and damage. The work includes a review of relevant literature as well as hands-on experimental verification of inspection capabilities. As nuclear power plants consider applying for second, or subsequent, license renewal to extend their operating period from 60 years to 80 years, it is important to understand how the materials installed in plant systems and components will age during that time and develop aging management programs to assuremore » continued safe operation under normal and design basis events (DBE). Normal component and system tests typically confirm the cables can perform their normal operational function. The focus of the cable test program, however, is directed toward the more demanding challenge of assuring the cable function under accident or DBE. The industry has adopted 50% elongation at break (EAB) relative to the un-aged cable condition as the acceptability standard. All tests are benchmarked against the cable EAB test. EAB, however, is a destructive test so the test programs must apply an array of other nondestructive examination (NDE) tests to assure or infer the overall set of cable’s system integrity. Assessment of cable integrity is further complicated in many cases by vendor’s use of dissimilar material for jacket and insulation. Frequently the jacket will degrade more rapidly than the underlying insulation. Although this can serve as an early alert to cable damage, direct test of the cable insulation without violating the protective jacket becomes problematic. This report addresses the range of bulk electrical NDE cable tests that are or could be practically implemented in a field-test situation with a particular focus on frequency domain reflectometry (FDR). The FDR test method offers numerous

Non-destructive analysis of sensory traits of dry-cured loins by MRI-computer vision techniques and data mining.

PubMed

Caballero, Daniel; Antequera, Teresa; Caro, Andrés; Ávila, María Del Mar; G Rodríguez, Pablo; Perez-Palacios, Trinidad

2017-07-01

Magnetic resonance imaging (MRI) combined with computer vision techniques have been proposed as an alternative or complementary technique to determine the quality parameters of food in a non-destructive way. The aim of this work was to analyze the sensory attributes of dry-cured loins using this technique. For that, different MRI acquisition sequences (spin echo, gradient echo and turbo 3D), algorithms for MRI analysis (GLCM, NGLDM, GLRLM and GLCM-NGLDM-GLRLM) and predictive data mining techniques (multiple linear regression and isotonic regression) were tested. The correlation coefficient (R) and mean absolute error (MAE) were used to validate the prediction results. The combination of spin echo, GLCM and isotonic regression produced the most accurate results. In addition, the MRI data from dry-cured loins seems to be more suitable than the data from fresh loins. The application of predictive data mining techniques on computational texture features from the MRI data of loins enables the determination of the sensory traits of dry-cured loins in a non-destructive way. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Rhizoslides: paper-based growth system for non-destructive, high throughput phenotyping of root development by means of image analysis.

PubMed

Le Marié, Chantal; Kirchgessner, Norbert; Marschall, Daniela; Walter, Achim; Hund, Andreas

2014-01-01

A quantitative characterization of root system architecture is currently being attempted for various reasons. Non-destructive, rapid analyses of root system architecture are difficult to perform due to the hidden nature of the root. Hence, improved methods to measure root architecture are necessary to support knowledge-based plant breeding and to analyse root growth responses to environmental changes. Here, we report on the development of a novel method to reveal growth and architecture of maize root systems. The method is based on the cultivation of different root types within several layers of two-dimensional, large (50 × 60 cm) plates (rhizoslides). A central plexiglass screen stabilizes the system and is covered on both sides with germination paper providing water and nutrients for the developing root, followed by a transparent cover foil to prevent the roots from falling dry and to stabilize the system. The embryonic roots grow hidden between a Plexiglas surface and paper, whereas crown roots grow visible between paper and the transparent cover. Long cultivation with good image quality up to 20 days (four fully developed leaves) was enhanced by suppressing fungi with a fungicide. Based on hyperspectral microscopy imaging, the quality of different germination papers was tested and three provided sufficient contrast to distinguish between roots and background (segmentation). Illumination, image acquisition and segmentation were optimised to facilitate efficient root image analysis. Several software packages were evaluated with regard to their precision and the time investment needed to measure root system architecture. The software 'Smart Root' allowed precise evaluation of root development but needed substantial user interference. 'GiaRoots' provided the best segmentation method for batch processing in combination with a good analysis of global root characteristics but overestimated root length due to thinning artefacts. 'WhinRhizo' offered the most rapid

Non-invasive and micro-destructive investigation of the Domus Aurea wall painting decorations.

PubMed

Clementi, Catia; Ciocan, Valeria; Vagnini, Manuela; Doherty, Brenda; Tabasso, Marisa Laurenzi; Conti, Cinzia; Brunetti, Brunetto Giovanni; Miliani, Costanza

2011-10-01

The paper reports on the exploitation of an educated multi-technique analytical approach based on a wide non invasive step followed by a focused micro-destructive step, aimed at the minimally invasive identification of the pigments decorating the ceiling of the Gilded Vault of the Domus Aurea in Rome. The combination of elemental analysis with molecular characterization provided by X-ray fluorescence and UV-vis spectroscopies, respectively, allowed for the in situ non-invasive identification of a remarkable number of pigments, namely Egyptian blue, green earth, cinnabar, red ochre and an anthraquinonic lake. The study was completed with the Raman analysis of two bulk samples, in particular, SERS measurements allowed for the speciation of the anthraquinonic pigment. Elemental mapping by scanning electron microscopy-energy dispersive spectrometer combined with micro-fluorimetry on cross-section gave an insight into both the distribution of different blend of pigments and on the nature of the inorganic support of the red dye.

Supercolor coding methods for large-scale multiplexing of biochemical assays.

PubMed

Rajagopal, Aditya; Scherer, Axel; Homyk, Andrew; Kartalov, Emil

2013-08-20

We present a novel method for the encoding and decoding of multiplexed biochemical assays. The method enables a theoretically unlimited number of independent targets to be detected and uniquely identified in any combination in the same sample. For example, the method offers easy access to 12-plex and larger PCR assays, as contrasted to the current 4-plex assays. This advancement would allow for large panels of tests to be run simultaneously in the same sample, saving reagents, time, consumables, and manual labor, while also avoiding the traditional loss of sensitivity due to sample aliquoting. Thus, the presented method is a major technological breakthrough with far-reaching impact on biotechnology, biomedical science, and clinical diagnostics. Herein, we present the mathematical theory behind the method as well as its experimental proof of principle using Taqman PCR on sequences specific to infectious diseases.

Conservation and restoration of natural building stones monitored through non-destructive X-ray computed tomography

NASA Astrophysics Data System (ADS)

Jacobs, P. Js; Cnudde, V.

2003-04-01

X-ray computed micro-tomography (μCT) is a promising non-destructive imaging technique to study building materials. μCT analysis provides information on the internal structure and petrophysical properties of small samples (size up to 2 cm diameter and 6 cm height), with to date a maximum resolution of 10 μm for commercial systems (Skyscan 1072). μCT allows visualising and measuring complete three-dimensional object structures without sample preparation. Possible applications of the μCT-technique for the monitoring of natural building stones are multiple: (i) to determine non-destructively porosity based on 3D images, (ii) to visualise weathering phenomena at the μ-scale, (iii) to understand the rationale of weathering processes, (iv) to visualise the presence of waterrepellents and consolidation products, (v) to monitor the protective effects of these products during weathering in order to understand the underlying weathering mechanisms and (vi) to provide advise on the suitability of products for the treatment of a particular rock-type. μCT-technique in combination with micro-Raman spectroscopy could prove to be a powerful tool for the future, as the combination of 3D visualisation and 2D chemical determination of inorganic as well as organic components could provide new insights to optimise conservation and restoration techniques of building materials. Determining the penetration depth of restoration products, used to consolidate or to protect natural building stones from weathering, is crucial if the application of conservation products is planned. Every type of natural building stone has its own petrophysical characteristics and each rock type reacts differently on the various restoration products available on the market. To assess the penetration depth and the effectiveness of a certain restoration product, μCT technology in combination with micro-Raman spectroscopy could be applied. Due to its non-destructive character and its resolution down to

Non-destructive fraud detection in rosehip oil by MIR spectroscopy and chemometrics.

PubMed

Santana, Felipe Bachion de; Gontijo, Lucas Caixeta; Mitsutake, Hery; Mazivila, Sarmento Júnior; Souza, Leticia Maria de; Borges Neto, Waldomiro

2016-10-15

Rosehip oil (Rosa eglanteria L.) is an important oil in the food, pharmaceutical and cosmetic industries. However, due to its high added value, it is liable to adulteration with other cheaper or lower quality oils. With this perspective, this work provides a new simple, fast and accurate methodology using mid-infrared (MIR) spectroscopy and partial least squares discriminant analysis (PLS-DA) as a means to discriminate authentic rosehip oil from adulterated rosehip oil containing soybean, corn and sunflower oils in different proportions. The model showed excellent sensitivity and specificity with 100% correct classification. Therefore, the developed methodology is a viable alternative for use in the laboratory and industry for standard quality analysis of rosehip oil since it is fast, accurate and non-destructive. Copyright © 2016 Elsevier Ltd. All rights reserved.

Improved Methods for Capture, Extraction, and Quantitative Assay of Environmental DNA from Asian Bigheaded Carp (Hypophthalmichthys spp.)

PubMed Central

Turner, Cameron R.; Miller, Derryl J.; Coyne, Kathryn J.; Corush, Joel

2014-01-01

Indirect, non-invasive detection of rare aquatic macrofauna using aqueous environmental DNA (eDNA) is a relatively new approach to population and biodiversity monitoring. As such, the sensitivity of monitoring results to different methods of eDNA capture, extraction, and detection is being investigated in many ecosystems and species. One of the first and largest conservation programs with eDNA-based monitoring as a central instrument focuses on Asian bigheaded carp (Hypophthalmichthys spp.), an invasive fish spreading toward the Laurentian Great Lakes. However, the standard eDNA methods of this program have not advanced since their development in 2010. We developed new, quantitative, and more cost-effective methods and tested them against the standard protocols. In laboratory testing, our new quantitative PCR (qPCR) assay for bigheaded carp eDNA was one to two orders of magnitude more sensitive than the existing endpoint PCR assays. When applied to eDNA samples from an experimental pond containing bigheaded carp, the qPCR assay produced a detection probability of 94.8% compared to 4.2% for the endpoint PCR assays. Also, the eDNA capture and extraction method we adapted from aquatic microbiology yielded five times more bigheaded carp eDNA from the experimental pond than the standard method, at a per sample cost over forty times lower. Our new, more sensitive assay provides a quantitative tool for eDNA-based monitoring of bigheaded carp, and the higher-yielding eDNA capture and extraction method we describe can be used for eDNA-based monitoring of any aquatic species. PMID:25474207

Improved methods for capture, extraction, and quantitative assay of environmental DNA from Asian bigheaded carp (Hypophthalmichthys spp.).

PubMed

Turner, Cameron R; Miller, Derryl J; Coyne, Kathryn J; Corush, Joel

2014-01-01

Indirect, non-invasive detection of rare aquatic macrofauna using aqueous environmental DNA (eDNA) is a relatively new approach to population and biodiversity monitoring. As such, the sensitivity of monitoring results to different methods of eDNA capture, extraction, and detection is being investigated in many ecosystems and species. One of the first and largest conservation programs with eDNA-based monitoring as a central instrument focuses on Asian bigheaded carp (Hypophthalmichthys spp.), an invasive fish spreading toward the Laurentian Great Lakes. However, the standard eDNA methods of this program have not advanced since their development in 2010. We developed new, quantitative, and more cost-effective methods and tested them against the standard protocols. In laboratory testing, our new quantitative PCR (qPCR) assay for bigheaded carp eDNA was one to two orders of magnitude more sensitive than the existing endpoint PCR assays. When applied to eDNA samples from an experimental pond containing bigheaded carp, the qPCR assay produced a detection probability of 94.8% compared to 4.2% for the endpoint PCR assays. Also, the eDNA capture and extraction method we adapted from aquatic microbiology yielded five times more bigheaded carp eDNA from the experimental pond than the standard method, at a per sample cost over forty times lower. Our new, more sensitive assay provides a quantitative tool for eDNA-based monitoring of bigheaded carp, and the higher-yielding eDNA capture and extraction method we describe can be used for eDNA-based monitoring of any aquatic species.

Can currently available non-animal methods detect pre and pro-haptens relevant for skin sensitization?

PubMed

Patlewicz, Grace; Casati, Silvia; Basketter, David A; Asturiol, David; Roberts, David W; Lepoittevin, Jean-Pierre; Worth, Andrew P; Aschberger, Karin

2016-12-01

Predictive testing to characterize substances for their skin sensitization potential has historically been based on animal tests such as the Local Lymph Node Assay (LLNA). In recent years, regulations in the cosmetics and chemicals sectors have provided strong impetus to develop non-animal alternatives. Three test methods have undergone OECD validation: the direct peptide reactivity assay (DPRA), the KeratinoSens™ and the human Cell Line Activation Test (h-CLAT). Whilst these methods perform relatively well in predicting LLNA results, a concern raised is their ability to predict chemicals that need activation to be sensitizing (pre- or pro-haptens). This current study reviewed an EURL ECVAM dataset of 127 substances for which information was available in the LLNA and three non-animal test methods. Twenty eight of the sensitizers needed to be activated, with the majority being pre-haptens. These were correctly identified by 1 or more of the test methods. Six substances were categorized exclusively as pro-haptens, but were correctly identified by at least one of the cell-based assays. The analysis here showed that skin metabolism was not likely to be a major consideration for assessing sensitization potential and that sensitizers requiring activation could be identified correctly using one or more of the current non-animal methods. Published by Elsevier Inc.

A new diatom growth inhibition assay using the XTT colorimetric method.

PubMed

Jiang, Weina; Akagi, Takuya; Suzuki, Hidekazu; Takimoto, Ayaka; Nagai, Hiroshi

2016-01-01

Marine biofouling, which leads to significant operational stress and economic damage on marine infrastructures, is a major problem in marine related industries. Currently, the most common way to avoid marine biofouling involves the use of biocidal products in surface coatings. However, the need for environmentally friendly antibiofouling compounds has increased rapidly with the recent global prohibition of harmful antifoulants, such as tributyltin (TBT). In particular, periphytic diatoms have been shown to contribute significantly to biofilms, which play an important role in biofouling. Therefore, inhibiting the proliferation of fouling diatoms is a very important step in the prevention of marine biofouling. In this study, we developed a new, rapid, accurate, and convenient growth inhibition assay using the XTT colorimetric method to prevent the growth of the fouling periphytic diatom, Nitzschia amabilis Hidek. Suzuki (replaced synonym, Nitzschia laevis Hustedt). The feasibility of this method was verified by determining the growth inhibition activities of two standard photosynthetic inhibitors, DCMU and CuSO4. However, neither inhibitor had any cytotoxic activities at the range of concentrations tested. Moreover, this method was applied by screening and purification of herbicidic but non-cytotoxic compounds from cyanobacteria extracts. Our results demonstrate the utility of this newly established growth inhibition assay for the identification of marine anti-biofouling compounds. Copyright © 2016 Elsevier Inc. All rights reserved.

Non-destructive prediction of pigment content in lettuce based on visible-NIR spectroscopy.

PubMed

Steidle Neto, Antonio José; Moura, Lorena de Oliveira; Lopes, Daniela de Carvalho; Carlos, Lanamar de Almeida; Martins, Luma Moreira; Ferraz, Leila de Castro Louback

2017-05-01

Lettuce (Lactuca sativa L.) is one of the most important salad vegetables in the world, with a number of head shapes, leaf types and colors. The lettuce pigments play important physiological functions, such as photosynthetic processes and light stress defense, but they also benefit human health because of their antioxidant action and anticarcinogenic properties. In this study three lettuce cultivars were grown under different farming systems, and partial least squares models were built to predict the leaf chlorophyll, carotenoid and anthocyanin content. The three proposed models resulted in high coefficients of determination and variable importance for the projection values, as well as low estimative errors for calibration and external validation datasets. These results confirmed that it is possible to accurately predict chlorophyll, carotenoid and anthocyanin content of green and red lettuces, grown in different farming systems, based on the spectral reflectance from 500 to 1000 nm. The proposed models were adequate for estimating lettuce pigments in a quick and non-destructive way, representing an alternative to conventional measurement methods. Prediction accuracies were improved by using the detrending, smoothing and first derivative pretreatments to the original spectral signatures prior to estimating lettuce chlorophyll, carotenoid and anthocyanin content, respectively. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Application of VNIR hyperspectral imaging for non-destructive prediction of pH, color, and drip loss of chicken breast fillets

USDA-ARS?s Scientific Manuscript database

Non-destructive and rapid prediction of quality attributes of chicken breast fillets using visible and near-infrared (VNIR) hyperspectral imaging (400-1000 nm) was carried out in this work. All hyperspectral images were acquired for bone (dorsal) side of chicken breast. A forward principal component...

Non-Destructive Trace Element Tomography Using Europe's Brightest Synchrotron Sources (ESRF-Grenoble, DESY-Hamburg) — Towards a Better Understanding of Martian Samples

NASA Astrophysics Data System (ADS)

Brenker, F. E.; Vincze, L.; Vekemans, B.; de Poulle, E.

2018-04-01

Synchrotron sources are valuable tools to measure the main and trace element content of extraterrestrial samples. The non-destructive measurements will allow to identify important geological processes within the martian mantle and crust.

The use of infrared thermal imaging as a non-destructive screening tool for identifying drought-tolerant lentil genotypes.

PubMed

Biju, Sajitha; Fuentes, Sigfredo; Gupta, Dorin

2018-06-01

Lentil (Lens culinaris, Medik.) is an important legume crop, which often experience drought stress especially at the flowering and grain filling phenological stages. The availability of efficient and robust screening tools based on relevant non-destructive quantifiable traits would facilitate research on crop improvement for drought tolerance. The objective of this study was to evaluate the drought tolerance of 37 lentil genotypes using infrared thermal imaging (IRTI), drought tolerance parameters and multivariate data analysis. Potted plants were kept in a completely randomized design in a growth chamber with five replicates. Plants were subjected to three different drought treatments: 100, 50 and 20% of field capacity at the onset of reproductive period. The relative drought stress tolerance was determined based on a set of morpho-physiological parameters including non-destructive measures based on IRTI, such as: canopy temperature (Tc), canopy temperature depression (CTD) and crop water stress index (CWSI) during the growing period and destructive measures at harvest, such as: dry root-shoot ratio (RS ratio), relative water content (RWC) and harvest index (HI). The drought tolerance indices used were drought susceptibility index (DSI) and drought tolerance efficiency (DTE). Results showed that drought stress treatments significantly reduced the RWC, HI, CTD and DSI, whereas, the values of Tc, CWSI, RS ratio and DTE significantly increased for all the genotypes. The cluster analysis from morpho-physiological parameters clustered genotypes in three distinctive groups as per the level of drought stress tolerance. The genotypes with higher values of RS ratio, RWC, HI, DTE and CTD and lower values of DSI, Tc and CWSI were identified as drought-tolerant genotypes. Based on this preliminary screening, the genotypes Digger, Cumra, Indianhead, ILL 5588, ILL 6002 and ILL 5582 were identified as promising drought-tolerant genotypes. It can be concluded that the IRTI

Non-destructive geochemical analysis and element mapping using bench-top μ-XRF: applications and uses for geoscience problems

NASA Astrophysics Data System (ADS)

Flude, Stephanie; Haschke, Michael; Tagle, Roald; Storey, Michael

2013-04-01

X-Ray Fluorescence (XRF) has long been used to provide valuable geochemical analysis of bulk rock samples in geological studies. However, it is a destructive technique, requiring samples to be homogenised by grinding to a fine powder and formed into a compacted pellet, or fused glass disk and the resulting sample has to be completely flat for reliable analysis. Until recently, non-destructive, high spatial resolution µ- XRF analysis was possible only at specialised Synchrotron radiation facilities, where high excitation beam energies are possible and specialised X-ray focussing optical systems are available. Recently, a number of bench-top µ-XRF systems have become available, allowing easy, rapid and non-destructive geochemical analysis of various materials. We present a number of examples of how the new bench-top M4 Tornado µ-XRF system, developed by Bruker Nano, can be used to provide valuable geochemical information on geological samples. Both quantitative and qualitative (in the form of X-Ray area-maps) data can be quickly and easily acquired for a wide range of elements (as light as Na, using a vacuum), with minimal sample preparation, using an X-Ray spot size as low as 25 µm. Large specimens up to 30 cm and 5 kg in weight can be analysed due to the large sample chamber, allowing non-destructive characterisation of rare or valuable materials. This technique is particularly useful in characterising heterogeneous samples, such as drill cores, sedimentary and pyroclastic rocks containing a variety of clasts, lavas sourced from mixed and mingled magmas, mineralised samples and fossils. An obvious application is the ability to produce element maps or line-scans of minerals, allowing zoning of major and trace elements to be identified and thus informing on crystallisation histories. An application of particular interest to 40Ar/39Ar geochronologists is the ability to screen and assess the purity of mineral separates, or to characterise polished slabs for

System and method for assaying a radionuclide

DOEpatents

Cadieux, James R; King, III, George S; Fugate, Glenn A

2014-12-23

A system for assaying a radionuclide includes a liquid scintillation detector, an analyzer connected to the liquid scintillation detector, and a delay circuit connected to the analyzer. A gamma detector and a multi-channel analyzer are connected to the delay circuit and the gamma detector. The multi-channel analyzer produces a signal reflective of the radionuclide in the sample. A method for assaying a radionuclide includes selecting a sample, detecting alpha or beta emissions from the sample with a liquid scintillation detector, producing a first signal reflective of the alpha or beta emissions, and delaying the first signal a predetermined time. The method further includes detecting gamma emissions from the sample, producing a second signal reflective of the gamma emissions, and combining the delayed first signal with the second signal to produce a third signal reflective of the radionuclide.

DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay.

PubMed

Susai, Surraj; Chand, Parkash; Ballambattu, Vishnu Bhat; Hanumanthappa, Nandeesha; Veeramani, Raveendranath

2016-05-01

Majority of the developmental delays in children are non-syndromic and they are believed to have an underlying DNA damage, though not well substantiated. Hence the present study was carried out to find out if there is any increased DNA damage in children with non-syndromic developmental delay by using the comet assay. The present case-control study was undertaken to assess the level of DNA damage in children with non syndromic developmental delay and compare the same with that of age and sex matched controls using submarine gel electrophoresis (Comet Assay). The blood from clinically diagnosed children with non syndromic developmental delay and controls were subjected for alkaline version of comet assay - Single cell gel electrophoresis using lymphocytes isolated from the peripheral blood. The comets were observed under a bright field microscope; photocaptured and scored using the Image J image quantification software. Comet parameters were compared between the cases and controls and statistical analysis and interpretation of results was done using the statistical software SPSS version 20. The mean comet tail length in cases and control was 20.77+7.659μm and 08.97+4.398μm respectively which was statistically significant (p<0.001). Other comet parameters like total comet length and % DNA in tail also showed a statistically significant difference (p < 0.001) between cases and controls. The current investigation unraveled increased levels of DNA damage in children with non syndromic developmental delay when compared to the controls.

Non-Destructive Characterization of Engineering Materials Using High-Energy X-rays at the Advanced Photon Source

DOE PAGES

Park, Jun-Sang; Okasinski, John; Chatterjee, Kamalika; ...

2017-05-30

High energy X-rays can penetrate large components and samples made from engineering alloys. Brilliant synchrotron sources like the Advanced Photon Source (APS) combined with unique experimental setups are increasingly allowing scientists and engineers to non-destructively characterize the state of materials across a range of length scales. In this article, some of the new developments at the APS, namely the high energy diffraction microscopy technique for grain-by-grain maps and aperture-based techniques for aggregate maps, are described.

Non-Destructive Characterization of Engineering Materials Using High-Energy X-rays at the Advanced Photon Source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Jun-Sang; Okasinski, John; Chatterjee, Kamalika

High energy X-rays can penetrate large components and samples made from engineering alloys. Brilliant synchrotron sources like the Advanced Photon Source (APS) combined with unique experimental setups are increasingly allowing scientists and engineers to non-destructively characterize the state of materials across a range of length scales. In this article, some of the new developments at the APS, namely the high energy diffraction microscopy technique for grain-by-grain maps and aperture-based techniques for aggregate maps, are described.

Grazing incidence synchrotron X-ray diffraction of marbles consolidated with diammonium hydrogen phosphate treatments: non-destructive probing of buried minerals

NASA Astrophysics Data System (ADS)

Possenti, Elena; Colombo, Chiara; Conti, Claudia; Gigli, Lara; Merlini, Marco; Plaisier, Jasper Rikkert; Realini, Marco; Gatta, G. Diego

2018-05-01

Diammonium hydrogen phosphate (DAP)-based consolidating treatments react with carbonatic stones and form calcium phosphates phases, whose composition depends on the availability of free calcium ions. In this work, an innovative non-destructive approach based on grazing incidence X-ray diffraction (GIXRD) with synchrotron radiation (SR) is used to investigate DAP-treated Carrara marble specimens and to study the influence of the substrate composition on the crystallization of calcium phosphate phases. The outcomes indicate that the presence of compositional micro-heterogeneity of Carrara marble favours the formation of specific phases. Dicalcium phosphate dihydrate, a calcium phosphate with a low Ca/P molar ratio, is formed on carbonatic phases with a low Ca amount, such as dolomite grains and Mg-containing veins. Furthermore, this study highlights the potentialities of SR-GIXRD as a powerful non-destructive tool for the diagnostic of Cultural Heritage objects since it allows investigating the conservation history of stone materials and their interaction with the environment.

Method for non-destructive evaluation of ceramic coatings

DOEpatents

Peterson, Kristen A.; Rosen, Elias P.; Jordan, Eric H.; Shahbazmohamadi, Sina; Vakhtin, Andrei B.

2016-11-08

A method for evaluating the condition of a ceramic coating deposited on a substrate comprising illuminating the ceramic coating with light, measuring the intensity of light returned from the ceramic coating as function of depth in the coating and transverse position on the coating, and analyzing the measured light intensities to obtain one or more of intensity of the light returned from the exposed coating surface relative to the intensity of light returned from the coating/substrate interface, intensity of the light returned from the coating/substrate interface relative to the intensity of light returned from the bulk of the ceramic coating, determination of roughness at the exposed surface of the ceramic coating, and determination of roughness of the interface between the ceramic coating and underlying bond coat or substrate.

Non-contact, non-destructive, quantitative probing of interfacial trap sites for charge carrier transport at semiconductor-insulator boundary

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choi, Wookjin; Miyakai, Tomoyo; Sakurai, Tsuneaki

The density of traps at semiconductor–insulator interfaces was successfully estimated using microwave dielectric loss spectroscopy with model thin-film organic field-effect transistors. The non-contact, non-destructive analysis technique is referred to as field-induced time-resolved microwave conductivity (FI-TRMC) at interfaces. Kinetic traces of FI-TRMC transients clearly distinguished the mobile charge carriers at the interfaces from the immobile charges trapped at defects, allowing both the mobility of charge carriers and the number density of trap sites to be determined at the semiconductor-insulator interfaces. The number density of defects at the interface between evaporated pentacene on a poly(methylmethacrylate) insulating layer was determined to be 10{supmore » 12 }cm{sup −2}, and the hole mobility was up to 6.5 cm{sup 2} V{sup −1} s{sup −1} after filling the defects with trapped carriers. The FI-TRMC at interfaces technique has the potential to provide rapid screening for the assessment of interfacial electronic states in a variety of semiconductor devices.« less

Can currently available non-animal methods detect pre and ...

EPA Pesticide Factsheets

Predictive testing to identify and characterise substances for their skin sensitisation potential has historically been based on animal tests such as the Local Lymph Node Assay (LLNA). In recent years, regulations in the cosmetics and chemicals sectors has provided a strong impetus to develop and evaluate non-animal alternative methods. The AOP for skin sensitisation provides a framework to anchor non-animal test methods to key events in the pathway to help identify what tests can be combined together to generate the potency information required for risk assessment. The 3 test methods that have undergone extensive development and validation are the direct peptide reactivity assay (DPRA), the KeratinoSensTM and the human Cell Line Activation Test (h-CLAT). Whilst these methods have been shown to perform relatively well in predicting LLNA results (accuracy ~ 80%), a particular concern that has been raised is their ability to predict chemicals that need to be activated to act as sensitisers (either abiotically on the skin (pre-hapten) or metabolically in the skin (pro-hapten)). The DPRA is a cell free system whereas the other two methods make use of cells that do not fully represent the in vivo metabolic situation. Based on previously published datasets of LLNA data, it has been found that approximately 25% of sensitisers are pre- and/or pro-haptens. This study reviewed an EURL ECVAM dataset of 127 substances for which information was available in the LLNA and the

Temporary formation of highly conducting domain walls for non-destructive read-out of ferroelectric domain-wall resistance switching memories

NASA Astrophysics Data System (ADS)

Jiang, Jun; Bai, Zi Long; Chen, Zhi Hui; He, Long; Zhang, David Wei; Zhang, Qing Hua; Shi, Jin An; Park, Min Hyuk; Scott, James F.; Hwang, Cheol Seong; Jiang, An Quan

2018-01-01

Erasable conductive domain walls in insulating ferroelectric thin films can be used for non-destructive electrical read-out of the polarization states in ferroelectric memories. Still, the domain-wall currents extracted by these devices have not yet reached the intensity and stability required to drive read-out circuits operating at high speeds. This study demonstrated non-destructive read-out of digital data stored using specific domain-wall configurations in epitaxial BiFeO3 thin films formed in mesa-geometry structures. Partially switched domains, which enable the formation of conductive walls during the read operation, spontaneously retract when the read voltage is removed, reducing the accumulation of mobile defects at the domain walls and potentially improving the device stability. Three-terminal memory devices produced 14 nA read currents at an operating voltage of 5 V, and operated up to T = 85 °C. The gap length can also be smaller than the film thickness, allowing the realization of ferroelectric memories with device dimensions far below 100 nm.

Non-animal methods to predict skin sensitization (II): an assessment of defined approaches *.

PubMed

Kleinstreuer, Nicole C; Hoffmann, Sebastian; Alépée, Nathalie; Allen, David; Ashikaga, Takao; Casey, Warren; Clouet, Elodie; Cluzel, Magalie; Desprez, Bertrand; Gellatly, Nichola; Göbel, Carsten; Kern, Petra S; Klaric, Martina; Kühnl, Jochen; Martinozzi-Teissier, Silvia; Mewes, Karsten; Miyazawa, Masaaki; Strickland, Judy; van Vliet, Erwin; Zang, Qingda; Petersohn, Dirk

2018-05-01

Skin sensitization is a toxicity endpoint of widespread concern, for which the mechanistic understanding and concurrent necessity for non-animal testing approaches have evolved to a critical juncture, with many available options for predicting sensitization without using animals. Cosmetics Europe and the National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods collaborated to analyze the performance of multiple non-animal data integration approaches for the skin sensitization safety assessment of cosmetics ingredients. The Cosmetics Europe Skin Tolerance Task Force (STTF) collected and generated data on 128 substances in multiple in vitro and in chemico skin sensitization assays selected based on a systematic assessment by the STTF. These assays, together with certain in silico predictions, are key components of various non-animal testing strategies that have been submitted to the Organization for Economic Cooperation and Development as case studies for skin sensitization. Curated murine local lymph node assay (LLNA) and human skin sensitization data were used to evaluate the performance of six defined approaches, comprising eight non-animal testing strategies, for both hazard and potency characterization. Defined approaches examined included consensus methods, artificial neural networks, support vector machine models, Bayesian networks, and decision trees, most of which were reproduced using open source software tools. Multiple non-animal testing strategies incorporating in vitro, in chemico, and in silico inputs demonstrated equivalent or superior performance to the LLNA when compared to both animal and human data for skin sensitization.

Manufacturing of reliable actively cooled fusion components - a challenge for non-destructive inspections

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reheis, N.; Zabernig, A.; Ploechl, L.

1994-12-31

Actively cooled in-vessel components like divertors or limiters require high quality and reliability to ensure safe operation during long term use. Such components are subjected to very severe thermal and mechanical cyclic loads and high power densities. Key requirements for materials in question are e.g. high melting point and thermal conductivity and low atomic mass number. Since no single material can simultaneously meet all of these requirements the selection of materials to be combined in composite components as well as of manufacturing and non-destructive inspection (NDI) methods is a particularly challenging task. Armour materials like graphite intended to face themore » plasma and help to maintain its desired properties, are bonded to metallic substrates like copper, molybdenum or stainless steel providing cooling and mechanical support. Several techniques such as brazing and active metal casting have been developed and successfully applied for joining materials with different thermophysical properties, pursuing the objective of sufficient heat dissipation from the hot, plasma facing surface to the coolant. NDI methods are an integral part of the manufacturing schedule of these components, starting in the design phase and ending in the final inspection. They apply all kinds of divertor types (monobloc and flat-tile concept). Particular focus is put on the feasibility of detecting small flaws and defects in complex interfaces and on the limits of these techniques. Special test pieces with defined defects acting as standards were inspected. Accompanying metallographic investigations were carried out to compare actual defects with results recorded during NDI.« less

Rotor assembly and assay method

DOEpatents

Burtis, C.A.; Johnson, W.F.; Walker, W.A.

1993-09-07

A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor. 34 figures.

Rotor assembly and assay method

DOEpatents

Burtis, Carl A.; Johnson, Wayne F.; Walker, William A.

1993-01-01

A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor.

A Quantitative, Non-Destructive Methodology for Habitat Characterisation and Benthic Monitoring at Offshore Renewable Energy Developments

PubMed Central

Sheehan, Emma V.; Stevens, Timothy F.; Attrill, Martin J.

2010-01-01

Following governments' policies to tackle global climate change, the development of offshore renewable energy sites is likely to increase substantially over coming years. All such developments interact with the seabed to some degree and so a key need exists for suitable methodology to monitor the impacts of large-scale Marine Renewable Energy Installations (MREIs). Many of these will be situated on mixed or rocky substrata, where conventional methods to characterise the habitat are unsuitable. Traditional destructive sampling is also inappropriate in conservation terms, particularly as safety zones around (MREIs) could function as Marine Protected Areas, with positive benefits for biodiversity. Here we describe a technique developed to effectively monitor the impact of MREIs and report the results of its field testing, enabling large areas to be surveyed accurately and cost-effectively. The methodology is based on a high-definition video camera, plus LED lights and laser scale markers, mounted on a “flying array” that maintains itself above the seabed grounded by a length of chain, thus causing minimal damage. Samples are taken by slow-speed tows of the gear behind a boat (200 m transects). The HD video and randomly selected frame grabs are analysed to quantify species distribution. The equipment was tested over two years in Lyme Bay, UK (25 m depth), then subsequently successfully deployed in demanding conditions at the deep (>50 m) high-energy Wave Hub site off Cornwall, UK, and a potential tidal stream energy site in Guernsey, Channel Islands (1.5 ms−1 current), the first time remote samples from such a habitat have been achieved. The next stage in the monitoring development process is described, involving the use of Remote Operated Vehicles to survey the seabed post-deployment of MREI devices. The complete methodology provides the first quantitative, relatively non-destructive method for monitoring mixed-substrate benthic communities beneath MPAs and

A quantitative, non-destructive methodology for habitat characterisation and benthic monitoring at offshore renewable energy developments.

PubMed

Sheehan, Emma V; Stevens, Timothy F; Attrill, Martin J

2010-12-29

Following governments' policies to tackle global climate change, the development of offshore renewable energy sites is likely to increase substantially over coming years. All such developments interact with the seabed to some degree and so a key need exists for suitable methodology to monitor the impacts of large-scale Marine Renewable Energy Installations (MREIs). Many of these will be situated on mixed or rocky substrata, where conventional methods to characterise the habitat are unsuitable. Traditional destructive sampling is also inappropriate in conservation terms, particularly as safety zones around (MREIs) could function as Marine Protected Areas, with positive benefits for biodiversity. Here we describe a technique developed to effectively monitor the impact of MREIs and report the results of its field testing, enabling large areas to be surveyed accurately and cost-effectively. The methodology is based on a high-definition video camera, plus LED lights and laser scale markers, mounted on a "flying array" that maintains itself above the seabed grounded by a length of chain, thus causing minimal damage. Samples are taken by slow-speed tows of the gear behind a boat (200 m transects). The HD video and randomly selected frame grabs are analysed to quantify species distribution. The equipment was tested over two years in Lyme Bay, UK (25 m depth), then subsequently successfully deployed in demanding conditions at the deep (>50 m) high-energy Wave Hub site off Cornwall, UK, and a potential tidal stream energy site in Guernsey, Channel Islands (1.5 ms⁻¹ current), the first time remote samples from such a habitat have been achieved. The next stage in the monitoring development process is described, involving the use of Remote Operated Vehicles to survey the seabed post-deployment of MREI devices. The complete methodology provides the first quantitative, relatively non-destructive method for monitoring mixed-substrate benthic communities beneath MPAs and MREIs

Using RT-PCR and bDNA assays to measure non-clade B HIV-1 subtype RNA.

PubMed

Pasquier, C; Sandres, K; Salama, G; Puel, J; Izopet, J

1999-08-01

The performance of the new version of RT-PCR assay (Amplicor HIV-1 Monitor v1.5) was assessed. The quantification of non-B subtype HIV-1 plasma RNA (30A, 1C, 1D, 3E, 2F, 3G) obtained using Monitor v1.5 was compared to the former version of this assay (Monitor v1.0) and to the Quantiplex v2.0 bDNA assay. The new primers used in Monitor v1.5 were similar to the former version in both specificity and sensitivity. The new primers corrected the detection and quantification defect observed previously for HIV-1 non-B subtypes and gave slightly higher RNA concentrations than those measured using the bDNA assay (+0.39 log copies/ml).

Advantage of using CBA/N strain mice in a non-radioisotopic modification of the local lymph node assay.

PubMed

Takeyoshi, Masahiro; Noda, Shuji; Yamasaki, Kanji; Kimber, Ian

2006-01-01

The murine local lymph node assay (LLNA) is currently recognized as a stand-alone test method for determining the skin sensitizing potential of chemicals. It has been incorporated into the official test guidelines published by some authorities, including the OECD. To avoid the use of radioisotopes, efforts have been made recently to develop non-radioisotopic modifications of the LLNA. A non-radioisotopic modification of the LLNA was developed previously using 5-bromo-2'-deoxyuridine (BrdU) incorporation (non-RI LLNA). However, the non-RI LLNA was found to be somewhat less sensitive than the standard assay. This study reports the advantage of using mice of the CBA/N strain in the non-RI LLNA to improve the sensitivity of this method. The non-RI LLNA was performed using CBA/JN and CBA/N mice exposed to one of four confirmed skin sensitizers, 2,4-dinitrochlorobenzene (DNCB), eugenol (EG), isoeugenol (IEG) or alpha-hexylcinnamic aldehyde (HCA), and to one non-sensitizer, propylene glycol (PG). The EC3 values for DNCB, IEG, EG, HCA and PG were calculated to be 0.1%, 9.6%, 40.6%, 45.5% and >50% in CBA/JN mice and 0.08%, 1.9%, 10.7%, 20.3% and >50% in CBA/N mice, respectively. The EC3 values for DNCB, IEG, EG, HCA and PG in the standard LLNA using CBA/Ca mice and radioisotopes were reported elsewhere as being 0.08%, 1.3%, 13.0%, 8.0% and >50%, respectively. The EC3 values derived from the CBA/N mice in the non-RI LLNA were nearly equivalent to the EC3 values obtained using the standard radioisotopic LLNA with CBA/Ca mice. These data suggest that the use of CBA/N mice may provide a realistic opportunity to develop a version of the LLNA that does not have a requirement for the use of radioisotopes, but which nevertheless has sensitivity approaching, or comparable to, the standard method. 2005 John Wiley & Sons, Ltd.

Non-destructive evaluation of nano-sized structure of thin film devices by using small angle neutron scattering.

PubMed

Shin, E J; Seong, B S; Choi, Y; Lee, J K

2011-01-01

Nano-sized multi-layers copper-doped SrZrO3, platinum (Pt) and silicon oxide (SiO2) on silicon substrates were prepared by dense plasma focus (DPF) device with the high purity copper anode tip and analyzed by using small angle neutron scattering (SANS) to establish a reliable method for the non-destructive evaluation of the under-layer structure. Thin film was well formed at the time-to-dip of 5 microsec with stable plasma of DPF. Several smooth intensity peaks were periodically observed when neutron beam penetrates the thin film with multi-layers perpendicularly. The platinum layer is dominant to intensity peaks, where the copper-doped SrZnO3 layer next to the platinum layer causes peak broadening. The silicon oxide layer has less effect on the SANS spectra due to its relative thick thickness. The SANS spectra shows thicknesses of platinum and copper-doped SrZnO3 layers as 53 and 25 nm, respectively, which are well agreement with microstructure observation.

Depth resolved compositional analysis of aluminium oxide thin film using non-destructive soft x-ray reflectivity technique

NASA Astrophysics Data System (ADS)

Sinha, Mangalika; Modi, Mohammed H.

2017-10-01

In-depth compositional analysis of 240 Å thick aluminium oxide thin film has been carried out using soft x-ray reflectivity (SXR) and x-ray photoelectron spectroscopy technique (XPS). The compositional details of the film is estimated by modelling the optical index profile obtained from the SXR measurements over 60-200 Å wavelength region. The SXR measurements are carried out at Indus-1 reflectivity beamline. The method suggests that the principal film region is comprised of Al2O3 and AlOx (x = 1.6) phases whereas the interface region comprised of SiO2 and AlOx (x = 1.6) mixture. The soft x-ray reflectivity technique combined with XPS measurements explains the compositional details of principal layer. Since the interface region cannot be analyzed with the XPS technique in a non-destructive manner in such a case the SXR technique is a powerful tool for nondestructive compositional analysis of interface region.

Quantitative Methods Based on Twisted Nematic Liquid Crystals for Mapping Surfaces Patterned with Bio/Chemical Functionality Relevant to Bioanalytical Assays

PubMed Central

Lowe, Aaron M.; Bertics, Paul J.; Abbott, Nicholas L.

2009-01-01

We report methods for the acquisition and analysis of optical images formed by thin films of twisted nematic liquid crystals (LCs) placed into contact with surfaces patterned with bio/chemical functionality relevant to surface-based assays. The methods are simple to implement and are shown to provide easily interpreted maps of chemical transformations on surfaces that are widely exploited in the preparation of analytic devices. The methods involve acquisition of multiple images of the LC as a function of the orientation of a polarizer; data analysis condenses the information present in the stack of images into a spatial map of the twist angle of the LC on the analytic surface. The potential utility of the methods is illustrated by mapping (i) the displacement of a monolayer formed from one alkanethiol on a gold film by a second thiol in solution, (ii) coadsorption of mixtures of amine-terminated and ethyleneglycol-terminated alkanethiols on gold films, which leads to a type of mixed monolayer that is widely exploited for immobilization of proteins on analytic surfaces, and (iii) patterns of antibodies printed onto surfaces. These results show that maps of the twist angle of the LC constructed from families of optical images can be used to reveal surface features that are not apparent in a single image of the LC film. Furthermore, the twist angles of the LC can be used to quantify the energy of interaction of the LC with the surface with a spatial resolution of <10 µm. When combined, the results described in this paper suggest non-destructive methods to monitor and validate chemical transformations on surfaces of the type that are routinely employed in the preparation of surface-based analytic technologies. PMID:18355089

Non-destructive and three-dimensional measurement of local strain development during tensile deformation in an aluminium alloy

NASA Astrophysics Data System (ADS)

Kobayashi, M.; Miura, H.; Toda, H.

2015-08-01

Anisotropy of mechanical responses depending on crystallographic orientation causes inhomogeneous deformation on the mesoscopic scale (grain size scale). Investigation of the local plastic strain development is important for discussing recrystallization mechanisms, because the sites with higher local plastic strain may act as potential nucleation sites for recrystallization. Recently, high-resolution X-ray tomography, which is non-destructive inspection method, has been utilized for observation of the materials structure. In synchrotron radiation X-ray tomography, more than 10,000 microstructural features, like precipitates, dispersions, compounds and hydrogen pores, can be observed in aluminium alloys. We have proposed employing these microstructural features as marker gauges to measure local strains, and then have developed a method to calculate the three-dimensional strain distribution by tracking the microstructural features. In this study, we report the development of local plastic strain as a function of the grain microstructure in an aluminium alloy by means of this three-dimensional strain measurement technique. Strongly heterogeneous strain development was observed during tensile loading to 30%. In other words, some parts of the sample deform little whereas another deforms a lot. However, strain in the whole specimen was keeping harmony. Comparing the microstructure with the strain concentration that is obtained by this method has a potential to reveal potential nucleation sites of recrystallization.

Destructive analysis capabilities for plutonium and uranium characterization at Los Alamos National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tandon, Lav; Kuhn, Kevin J; Drake, Lawrence R

Los Alamos National Laboratory's (LANL) Actinide Analytical Chemistry (AAC) group has been in existence since the Manhattan Project. It maintains a complete set of analytical capabilities for performing complete characterization (elemental assay, isotopic, metallic and non metallic trace impurities) of uranium and plutonium samples in different forms. For a majority of the customers there are strong quality assurance (QA) and quality control (QC) objectives including highest accuracy and precision with well defined uncertainties associated with the analytical results. Los Alamos participates in various international and national programs such as the Plutonium Metal Exchange Program, New Brunswick Laboratory's (NBL' s) Safeguardsmore » Measurement Evaluation Program (SME) and several other inter-laboratory round robin exercises to monitor and evaluate the data quality generated by AAC. These programs also provide independent verification of analytical measurement capabilities, and allow any technical problems with analytical measurements to be identified and corrected. This presentation will focus on key analytical capabilities for destructive analysis in AAC and also comparative data between LANL and peer groups for Pu assay and isotopic analysis.« less

Energy-selective Neutron Imaging for Three-dimensional Non-destructive Probing of Crystalline Structures

NASA Astrophysics Data System (ADS)

Peetermans, S.; Bopp, M.; Vontobel, P.; Lehmann, E. H.

Common neutron imaging uses the full polychromatic neutron beam spectrum to reveal the material distribution in a non-destructive way. Performing it with a reduced energy band, i.e. energy-selective neutron imaging, allows access to local variation in sample crystallographic properties. Two sample categories can be discerned with different energy responses. Polycrystalline materials have an energy-dependent cross-section featuring Bragg edges. Energy-selective neutron imaging can be used to distinguish be- tween crystallographic phases, increase material sensitivity or penetration, improve quantification etc. An example of the latter is shown by the examination of copper discs prior to machining them into linear accelerator cavity structures. The cross-section of single crystals features distinct Bragg peaks. Based on their pattern, one can determine the orientation of the crystal, as in a Laue pattern, but with the tremendous advantage that the operation can be performed for each pixel, yielding crystal orientation maps at high spatial resolution. A wholly different method to investigate such samples is also introduced: neutron diffraction imaging. It is based on projections formed by neutrons diffracted from the crystal lattice out of the direct beam. The position of these projections on the detector gives information on the crystal orientation. The projection itself can be used to reconstruct the crystal shape. A three-dimensional mapping of local Bragg reflectivity or a grain orientation mapping can thus be obtained.

Routes for GMR-Sensor Design in Non-Destructive Testing

PubMed Central

Pelkner, Matthias; Neubauer, Andreas; Reimund, Verena; Kreutzbruck, Marc; Schütze, Andreas

2012-01-01

GMR sensors are widely used in many industrial segments such as information technology, automotive, automation and production, and safety applications. Each area requires an adaption of the sensor arrangement in terms of size adaption and alignment with respect to the field source involved. This paper deals with an analysis of geometric sensor parameters and the arrangement of GMR sensors providing a design roadmap for non-destructive testing (NDT) applications. For this purpose we use an analytical model simulating the magnetic flux leakage (MFL) distribution of surface breaking defects and investigate the flux leakage signal as a function of various sensor parameters. Our calculations show both the influence of sensor length and height and that when detecting the magnetic flux leakage of μm sized defects a gradiometer base line of 250 μm leads to a signal strength loss of less than 10% in comparison with a magnetometer response. To validate the simulation results we finally performed measurements with a GMR magnetometer sensor on a test plate with artificial μm-range cracks. The differences between simulation and measurement are below 6%. We report on the routes for a GMR gradiometer design as a basis for the fabrication of NDT-adapted sensor arrays. The results are also helpful for the use of GMR in other application when it comes to measure positions, lengths, angles or electrical currents.

Non-destructive identification of unknown minor phases in polycrystalline bulk alloys using three-dimensional X-ray diffraction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Yiming, E-mail: yangyiming1988@outlook.com

Minor phases make considerable contributions to the mechanical and physical properties of metals and alloys. Unfortunately, it is difficult to identify unknown minor phases in a bulk polycrystalline material using conventional metallographic methods. Here, a non-destructive method based on three-dimensional X-ray diffraction (3DXRD) is developed to solve this problem. Simulation results demonstrate that this method is simultaneously able to identify minor phase grains and reveal their positions, orientations and sizes within bulk alloys. According to systematic simulations, the 3DXRD method is practicable for an extensive sample set, including polycrystalline alloys with hexagonal, orthorhombic and cubic minor phases. Experiments were alsomore » conducted to confirm the simulation results. The results for a bulk sample of aluminum alloy AA6061 show that the crystal grains of an unexpected γ-Fe (austenite) phase can be identified, three-dimensionally and nondestructively. Therefore, we conclude that the 3DXRD method is a powerful tool for the identification of unknown minor phases in bulk alloys belonging to a variety of crystal systems. This method also has the potential to be used for in situ observations of the effects of minor phases on the crystallographic behaviors of alloys. - Highlights: •A method based on 3DXRD is developed for identification of unknown minor phase. •Grain position, orientation and size, is simultaneously acquired. •A systematic simulation demonstrated the applicability of the proposed method. •Experimental results on a AA6061 sample confirmed the practicability of the method.« less

Bioprocessing development: Immune/cellular applications: Anti-Ig autoantibody and complement-mediated destruction of neoplastic cells

NASA Technical Reports Server (NTRS)

Twomey, J. J.

1976-01-01

This space bioprocessing contract effort was comprised of four general objectives. These were: (1) the evaluation of current separation processes, (2) the identification of problems relevant to the separation of important biologicals, (3) the identification of ground-based assay methods needed for pre- and postflight analysis of space bioprocessing separation technology; and (4) the establishment of methods to determine the efficiency of space bioprocessing separation procedures. Immunology was deemed advantageous to study the diversity of cells and cell products involved and the extensive interest being given to their separation. Upon recognition of a cellular or molecular agent as foreign to the body, the immune system becomes activated to produce cells whose function is to destroy that agent and cell products whose function is to inactivate the agent and assist in its destruction. Long after the agent is removed from the body, some cells remain in a state of readiness to continue these destructive actions specifically against that agent should further exposure to it occur. This is the basis of acquired immunity to disease.

Proposal and proof-of-principle demonstration of non-destructive detection of photonic qubits using a Tm:LiNbO3 waveguide

PubMed Central

Sinclair, N.; Heshami, K.; Deshmukh, C.; Oblak, D.; Simon, C.; Tittel, W.

2016-01-01

Non-destructive detection of photonic qubits is an enabling technology for quantum information processing and quantum communication. For practical applications, such as quantum repeaters and networks, it is desirable to implement such detection in a way that allows some form of multiplexing as well as easy integration with other components such as solid-state quantum memories. Here, we propose an approach to non-destructive photonic qubit detection that promises to have all the mentioned features. Mediated by an impurity-doped crystal, a signal photon in an arbitrary time-bin qubit state modulates the phase of an intense probe pulse that is stored during the interaction. Using a thulium-doped waveguide in LiNbO3, we perform a proof-of-principle experiment with macroscopic signal pulses, demonstrating the expected cross-phase modulation as well as the ability to preserve the coherence between temporal modes. Our findings open the path to a new key component of quantum photonics based on rare-earth-ion-doped crystals. PMID:27853153

Non-destructive Determination of Disintegration Time and Dissolution in Immediate Release Tablets by Terahertz Transmission Measurements.

PubMed

Markl, Daniel; Sauerwein, Johanna; Goodwin, Daniel J; van den Ban, Sander; Zeitler, J Axel

2017-05-01

measure content uniformity and the fact that the method requires no chemometric models this technology shows clear promise to be established as a process analyser to non-destructively predict critical quality attributes of tablets.

Immunofluorescence assay method to detect dengue virus in Paniai-Papua

NASA Astrophysics Data System (ADS)

Sucipto, Teguh Hari; Ahwanah, Nur Laila Fitriati; Churrotin, Siti; Matake, Norifumi; Kotaki, Tomohiro; Soegijanto, Soegeng

2016-03-01

The dengue viruses (DENV), which include in the family Flaviviridae and the genus Flavivirus, was endemic in tropical areas and had been transmitted to humans by Aedes aegypti. An increasing number of immigrants from endemic areas to the non-endemic areas have emphasized the need for a simple and reliable test for the diagnosis of dengue virus infection. The purpose of this study was to detect the dengue virus by immunofluorescence assay (IFA) in the general population at Paniai-Papua. The results obtained from this study had showed a significantly better discrimination for DENV specific IgG antibodies. A total of 158 samples, 116 samples were IgG antibodies positive and 42 samples were negative. The conclusion of this study, Papua is not only a malaria endemic area, but also dengue virus infections were detected by IFA method. Therefore, the IFA can be used as an important diagnostic tool, which is a quick and an easy way to test samples from immigrants who come to the non-endemic areas.

Multiplex Quantitative PCR Assays for the Detection and Quantification of the Six Major Non-O157 Escherichia coli Serogroups in Cattle Feces.

PubMed

Shridhar, P B; Noll, L W; Shi, X; An, B; Cernicchiaro, N; Renter, D G; Nagaraja, T G; Bai, J

2016-01-01

Shiga toxin-producing Escherichia coli (STEC) serogroups O26, O45, O103, O111, O121, and O145, called non-O157 STEC, are important foodborne pathogens. Cattle, a major reservoir, harbor the organisms in the hindgut and shed them in the feces. Although limited data exist on fecal shedding, concentrations of non-O157 STEC in feces have not been reported. The objectives of our study were (i) to develop and validate two multiplex quantitative PCR (mqPCR) assays, targeting O-antigen genes of O26, O103, and O111 (mqPCR-1) and O45, O121, and O145 (mqPCR-2); (ii) to utilize the two assays, together with a previously developed four-plex qPCR assay (mqPCR-3) targeting the O157 antigen and three virulence genes (stx1, stx2, and eae), to quantify seven serogroups and three virulence genes in cattle feces; and (iii) to compare the three mqPCR assays to a 10-plex conventional PCR (cPCR) targeting seven serogroups and three virulence genes and culture methods to detect seven E. coli serogroups in cattle feces. The two mqPCR assays (1 and 2) were shown to be specific to the target genes, and the detection limits were 4 and 2 log CFU/g of pure culture-spiked fecal samples, before and after enrichment, respectively. A total of 576 fecal samples collected from a feedlot were enriched in E. coli broth and were subjected to quantification (before enrichment) and detection (after enrichment). Of the 576 fecal samples subjected, before enrichment, to three mqPCR assays for quantification, 175 (30.4%) were quantifiable (≥4 log CFU/g) for at least one of the seven serogroups, with O157 being the most common serogroup. The three mqPCR assays detected higher proportions of postenriched fecal samples (P > 0.01) as positive for one or more serogroups compared with cPCR and culture methods. This is the first study to assess the applicability of qPCR assays to detect and quantify six non-O157 serogroups in cattle feces and to generate data on fecal concentration of the six serogroups.

Methods and devices for protein assays

DOEpatents

Chhabra, Swapnil [San Jose, CA; Cintron, Jose M [Indianapolis, IN; Shediac, Renee [Oakland, CA

2009-11-03

Methods and devices for protein assays based on Edman degradation in microfluidic channels are disclosed herein. As disclosed, the cleaved amino acid residues may be immobilized in an array format and identified by detectable labels, such as antibodies, which specifically bind given amino acid residues. Alternatively, the antibodies are immobilized in an array format and the cleaved amino acids are labeled identified by being bound by the antibodies in the array.

Non-destructive analysis of museum objects by fibre-optic Raman spectroscopy

PubMed Central

Tate, Jim; Moens, Luc

2006-01-01

Raman spectroscopy is a versatile technique that has frequently been applied for the investigation of art objects. By using mobile Raman instrumentation it is possible to investigate the artworks without the need for sampling. This work evaluates the use of a dedicated mobile spectrometer for the investigation of a range of museum objects in museums in Scotland, including antique Egyptian sarcophagi, a panel painting, painted surfaces on paper and textile, and the painted lid and soundboard of an early keyboard instrument. The investigations of these artefacts illustrate some analytical challenges that arise when analysing museum objects, including fluorescing varnish layers, ambient sunlight, large dimensions of artefacts and the need to handle fragile objects with care. Analysis of the musical instrument (the Mar virginals) was undertaken in the exhibition gallery, while on display, which meant that interaction with the public and health and safety issues had to be taken into account. Experimental set-up for the non-destructive Raman spectroscopic investigation of a textile banner in the National Museums of Scotland PMID:16953310

Non-destructive analysis of museum objects by fibre-optic Raman spectroscopy.

PubMed

Vandenabeele, Peter; Tate, Jim; Moens, Luc

2007-02-01

Raman spectroscopy is a versatile technique that has frequently been applied for the investigation of art objects. By using mobile Raman instrumentation it is possible to investigate the artworks without the need for sampling. This work evaluates the use of a dedicated mobile spectrometer for the investigation of a range of museum objects in museums in Scotland, including antique Egyptian sarcophagi, a panel painting, painted surfaces on paper and textile, and the painted lid and soundboard of an early keyboard instrument. The investigations of these artefacts illustrate some analytical challenges that arise when analysing museum objects, including fluorescing varnish layers, ambient sunlight, large dimensions of artefacts and the need to handle fragile objects with care. Analysis of the musical instrument (the Mar virginals) was undertaken in the exhibition gallery, while on display, which meant that interaction with the public and health and safety issues had to be taken into account. Experimental set-up for the non-destructive Raman spectroscopic investigation of a textile banner in the National Museums of Scotland.

PHOTOACOUSTIC NON-DESTRUCTIVE EVALUATION AND IMAGING OF CARIES IN DENTAL SAMPLES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, T.; Dewhurst, R. J.

Dental caries is a disease wherein bacterial processes damage hard tooth structure. Traditional dental radiography has its limitations for detecting early stage caries. In this study, a photoacoustic (PA) imaging system with the near-infrared light source has been applied to postmortem dental samples to obtain 2-D and 3-D images. Imaging results showed that the PA technique can be used to image human teeth caries. For non-destructive photoacoustic evaluation and imaging, the induced temperature and pressure rises within biotissues should not cause physical damage to the tissue. For example, temperature rises above 5 deg. C within live human teeth will causemore » pulpal necrosis. Therefore, several simulations based on the thermoelastic effect have been applied to predict temperature and pressure fields within samples. Predicted temperature levels are below corresponding safety limits, but care is required to avoid nonlinear absorption phenomena. Furthermore, PA imaging results from the phantom provide evidence for high sensitivity, which shows the imaging potential of the PA technique for detecting early stage disease.« less

Photoacoustic Non-Destructive Evaluation and Imaging of Caries in Dental Samples

NASA Astrophysics Data System (ADS)

Li, T.; Dewhurst, R. J.

2010-02-01

Dental caries is a disease wherein bacterial processes damage hard tooth structure. Traditional dental radiography has its limitations for detecting early stage caries. In this study, a photoacoustic (PA) imaging system with the near-infrared light source has been applied to postmortem dental samples to obtain 2-D and 3-D images. Imaging results showed that the PA technique can be used to image human teeth caries. For non-destructive photoacoustic evaluation and imaging, the induced temperature and pressure rises within biotissues should not cause physical damage to the tissue. For example, temperature rises above 5 °C within live human teeth will cause pulpal necrosis. Therefore, several simulations based on the thermoelastic effect have been applied to predict temperature and pressure fields within samples. Predicted temperature levels are below corresponding safety limits, but care is required to avoid nonlinear absorption phenomena. Furthermore, PA imaging results from the phantom provide evidence for high sensitivity, which shows the imaging potential of the PA technique for detecting early stage disease.

Evaluation of the veracity of one work by the artist Di Cavalcanti through non-destructive techniques: XRF, imaging and brush stroke analysis

NASA Astrophysics Data System (ADS)

Kajiya, E. A. M.; Campos, P. H. O. V.; Rizzutto, M. A.; Appoloni, C. R.; Lopes, F.

2014-02-01

This paper presents systematic studies and analysis that contributed to the identification of the forgery of a work by the artist Emiliano Augusto Cavalcanti de Albuquerque e Melo, known as Di Cavalcanti. The use of several areas of expertise such as brush stroke analysis ("pinacologia"), applied physics, and art history resulted in an accurate diagnosis for ascertaining the authenticity of the work entitled "Violeiro" (1950). For this work we used non-destructive methods such as techniques of infrared, ultraviolet, visible and tangential light imaging combined with chemical analysis of the pigments by portable X-Ray Fluorescence (XRF) and graphic gesture analysis. Each applied method of analysis produced specific information that made possible the identification of materials and techniques employed and we concluded that this work is not consistent with patterns characteristic of the artist Di Cavalcanti.

Static characterization of a soft elastomeric capacitor for non destructive evaluation applications

NASA Astrophysics Data System (ADS)

Saleem, Hussam; Laflamme, Simon; Zhang, Huanhuan; Geiger, Randall; Kessler, Michael; Rajan, Krishna

2014-02-01

A large and flexible strain transducer consisting of a soft elastomeric capacitor (SEC) has been proposed by the authors. Arranged in a network setup, the sensing strategy offers tremendous potential at conducting non-destructive evaluation of large-scale surfaces. In prior work, the authors have demonstrated the performance of the sensor at tracking strain history, localizing cracks, and detecting vibration signatures. In this paper, we characterize the static performance of the proposed SEC. The characterization includes sensitivity of the signal, and temperature and humidity dependences. Tests are conducted on a simply supported aluminum beam subjected to bending as well as on a free standing sensor. The performance of the SEC is compared against off-the-shelf resistance-based strain gauges with resolution of 1 μɛ. A sensitivity of 1190 pF/ɛ is obtained experimentally, in agreement with theory. Results also show the sensor linearity over the given level of strain, showing the promise of the SEC at monitoring of surface strain.

Unmanned Ground Vehicle for Autonomous Non-Destructive Testing of FRP Bridge Decks

NASA Astrophysics Data System (ADS)

Klinkhachorn, P.; Mercer, A. Scott; Halabe, Udaya B.; GangaRao, Hota V. S.

2007-03-01

Current non-destructive techniques for defect analysis of FRP bridge decks have a narrow scope. These techniques are very good at detecting certain types of defects but are not robust enough to detect all defects by themselves. For example, infrared thermography (IRT) can detect air filled defects and Ground Penetrating Radar (GPR) is good at detecting water filled ones. These technologies can be combined to create a more robust defect detection scheme. To accomplish this, an Unmanned Ground Vehicle (UGV) has been designed that incorporates both IR and GPR analysis to create a comprehensive defect map of a bridge deck. The UGV autonomously surveys the deck surface and acquires data. The UGV has two 1.5 GHz ground coupled GPR antennas that are mounted on the front of the UGV to collect GPR data. It also incorporates an active heating source and a radiometric IR camera to capture IR images of the deck, even in less than ideal weather scenarios such as cold cloudy days. The UGV is designed so that it can collect data in an assembly line fashion. It moves in 1 foot increments. When moving, it collects GPR data from the two antennas. When it stops it heats a section of the deck. The next time it stops to heat a section, the IR camera is analyzing the preheated deck section while preparing for the next section. Because the data is being continually collected using this method, the UGV can survey the entire deck in an efficient and timely manner.

Genotoxicity assessment of nanomaterials: recommendations on best practices, assays and methods.

PubMed

Elespuru, Rosalie; Pfuhler, Stefan; Aardema, Marilyn; Chen, Tao; Doak, Shareen H; Doherty, Ann; Farabaugh, Christopher S; Kenny, Julia; Manjanatha, Mugimane; Mahadevan, Brinda; Moore, Martha M; Ouédraogo, Gladys; Stankowski, Leon F; Tanir, Jennifer Y

2018-04-26

Nanomaterials (NMs) present unique challenges in safety evaluation. An international working group, the Genetic Toxicology Technical Committee of the International Life Sciences Institute's Health and Environmental Sciences Institute, has addressed issues related to the genotoxicity assessment of NMs. A critical review of published data has been followed by recommendations on methods alterations and best practices for the standard genotoxicity assays: bacterial reverse mutation (Ames); in vitro mammalian assays for mutations, chromosomal aberrations, micronucleus induction, or DNA strand breaks (comet); and in vivo assays for genetic damage (micronucleus, comet and transgenic mutation assays). The analysis found a great diversity of tests and systems used for in vitro assays; many did not meet criteria for a valid test, and/or did not use validated cells and methods in the Organization for Economic Co-operation and Development Test Guidelines, and so these results could not be interpreted. In vivo assays were less common but better performed. It was not possible to develop conclusions on test system agreement, NM activity, or mechanism of action. However, the limited responses observed for most NMs were consistent with indirect genotoxic effects, rather than direct interaction of NMs with DNA. We propose a revised genotoxicity test battery for NMs that includes in vitro mammalian cell mutagenicity and clastogenicity assessments; in vivo assessments would be added only if warranted by information on specific organ exposure or sequestration of NMs. The bacterial assays are generally uninformative for NMs due to limited particle uptake and possible lack of mechanistic relevance, and are thus omitted in our recommended test battery for NM assessment. Recommendations include NM characterization in the test medium, verification of uptake into target cells, and limited assay-specific methods alterations to avoid interference with uptake or endpoint analysis. These

Non-destructive testing method and apparatus utilizing phase multiplication holography

DOEpatents

Collins, H. Dale; Prince, James M.; Davis, Thomas J.

1984-01-01

An apparatus and method for imaging of structural characteristics in test objects using radiation amenable to coherent signal processing methods. Frequency and phase multiplication of received flaw signals is used to simulate a test wavelength at least one to two orders of magnitude smaller than the actual wavelength. The apparent reduction in wavelength between the illumination and recording radiation performs a frequency translation hologram. The hologram constructed with a high synthetic frequency and flaw phase multiplication is similar to a conventional acoustic hologram construction at the high frequency.

Non-destructive X-ray examination of weft knitted wire structures

NASA Astrophysics Data System (ADS)

Obermann, M.; Ellouz, M.; Aumann, S.; Martens, Y.; Bartelt, P.; Klöcker, M.; Kordisch, T.; Ehrmann, A.; Weber, M. O.

2016-07-01

Conductive yarns or wires are often integrated in smart textiles to enable data or energy transmission. In woven fabrics, these conductive parts are fixed at defined positions and thus protected from external loads. Knitted fabrics, however, have relatively loose structures, resulting in higher impacts of possible mechanical forces on the individual yarns. Hence, metallic wires with smaller diameters in particular are prone to break when integrated in knitted fabrics. In a recent project, wires of various materials including copper, silver and nickel with diameters varying between 0.05 mm and 0.23 mm were knitted in combination with textile yarns. Hand flat knitting machines of appropriate gauges were used to produce different structures. On these samples, non-destructive examinations, using an industrial X-ray system Seifert x|cube (225 kV) equipped with a minifocus X-ray tube, were carried out, directly after knitting as well as after different mechanical treatments (tensile, burst, and washing tests). In this way, structural changes of the stitch geometry could be visualized before failure. In this paper, the loop geometries in the knitted fabrics are depicted depending on knitted structures, wire properties and the applied mechanical load. Consequently, it is shown which metallic wires and yarns are most suitable to be integrated into knitted smart textiles.

Improving LiDAR Biomass Model Uncertainty through Non-Destructive Allometry and Plot-level 3D Reconstruction with Terrestrial Laser Scanning

NASA Astrophysics Data System (ADS)

Stovall, A. E.; Shugart, H. H., Jr.

2017-12-01

Future NASA and ESA satellite missions plan to better quantify global carbon through detailed observations of forest structure, but ultimately rely on uncertain ground measurement approaches for calibration and validation. A significant amount of the uncertainty in estimating plot-level biomass can be attributed to inadequate and unrepresentative allometric relationships used to convert plot-level tree measurements to estimates of aboveground biomass. These allometric equations are known to have high errors and biases, particularly in carbon rich forests because they were calibrated with small and often biased samples of destructively harvested trees. To overcome this issue, a non-destructive methodology for estimating tree and plot-level biomass has been proposed through the use of Terrestrial Laser Scanning (TLS). We investigated the potential for using TLS as a ground validation approach in LiDAR-based biomass mapping though virtual plot-level tree volume reconstruction and biomass estimation. Plot-level biomass estimates were compared on the Virginia-based Smithsonian Conservation Biology Institute's SIGEO forest with full 3D reconstruction, TLS allometry, and Jenkins et al. (2003) allometry. On average, full 3D reconstruction ultimately provided the lowest uncertainty estimate of plot-level biomass (9.6%), followed by TLS allometry (16.9%) and the national equations (20.2%). TLS offered modest improvements to the airborne LiDAR empirical models, reducing RMSE from 16.2% to 14%. Our findings suggest TLS plot acquisitions and non-destructive allometry can play a vital role for reducing uncertainty in calibration and validation data for biomass mapping in the upcoming NASA and ESA missions.

Hypothyroidism presenting as destructive arthropathy of the fingers.

PubMed Central

Gerster, J. C.; Quadri, P.; Saudan, Y.

1985-01-01

A patient presenting with destructive arthropathy of the proximal interphalangeal (PIP) joints of the hands is described. She was initially believed to have rheumatoid arthritis but non-steroidal anti-inflammatory drugs were of no help. The patient was subsequently found to have hypothyroidism and erosive osteoarthritis of the fingers. Joint swelling, pain and stiffness responded dramatically to thyroid hormone substitution. The PIP joint spaces reappeared on the radiographs within 9 months. This case suggest that hypothyroidism may induce destructive arthropathy of the finger joints. As thyroxine replacement may reverse the rheumatic complaints, hypothyroidism should be considered in the differential diagnosis of a destructive arthropathy of unclear aetiology. Images Figure 1 Figure 2 PMID:3983045

A life cycle assessment of destruction of ammunition.

PubMed

Alverbro, K; Björklund, A; Finnveden, G; Hochschorner, E; Hägvall, J

2009-10-30

The Swedish Armed Forces have large stocks of ammunition that were produced at a time when decommissioning was not considered. This ammunition will eventually become obsolete and must be destroyed, preferably with minimal impact on the environment and in a safe way for personnel. The aim of this paper is to make a comparison of the environmental impacts in a life cycle perspective of three different methods of decommissioning/destruction of ammunition, and to identify the environmental advantages and disadvantages of each of these destruction methods: open detonation; static kiln incineration with air pollution control combined with metal recycling, and a combination of incineration with air pollution control, open burning, recovery of some energetic material and metal recycling. Data used are for the specific processes and from established LCA databases. Recycling the materials in the ammunition and minimising the spread of airborne pollutants during incineration were found to be the most important factors affecting the life cycle environmental performance of the compared destruction methods. Open detonation with or without metal recycling proved to be the overall worst alternative from a life cycle perspective. The results for the static kiln and combination treatment indicate that the kind of ammunition and location of the destruction plant might determine the choice of method, since the environmental impacts from these methods are of little difference in the case of this specific grenade. Different methods for destruction of ammunition have previously been discussed from a risk and safety perspective. This is however to our knowledge the first study looking specifically on environmentally aspect in a life cycle perspective.

Improved uncertainty quantification in nondestructive assay for nonproliferation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Burr, Tom; Croft, Stephen; Jarman, Ken

2016-12-01

This paper illustrates methods to improve uncertainty quantification (UQ) for non-destructive assay (NDA) measurements used in nuclear nonproliferation. First, it is shown that current bottom-up UQ applied to calibration data is not always adequate, for three main reasons: (1) Because there are errors in both the predictors and the response, calibration involves a ratio of random quantities, and calibration data sets in NDA usually consist of only a modest number of samples (3–10); therefore, asymptotic approximations involving quantities needed for UQ such as means and variances are often not sufficiently accurate; (2) Common practice overlooks that calibration implies a partitioningmore » of total error into random and systematic error, and (3) In many NDA applications, test items exhibit non-negligible departures in physical properties from calibration items, so model-based adjustments are used, but item-specific bias remains in some data. Therefore, improved bottom-up UQ using calibration data should predict the typical magnitude of item-specific bias, and the suggestion is to do so by including sources of item-specific bias in synthetic calibration data that is generated using a combination of modeling and real calibration data. Second, for measurements of the same nuclear material item by both the facility operator and international inspectors, current empirical (top-down) UQ is described for estimating operator and inspector systematic and random error variance components. A Bayesian alternative is introduced that easily accommodates constraints on variance components, and is more robust than current top-down methods to the underlying measurement error distributions.« less

Assay of fluoxetine hydrochloride by titrimetric and HPLC methods.

PubMed

Bueno, F; Bergold, A M; Fröehlich, P E

2000-01-01

Two alternative methods were proposed to assay Fluoxetine Hydrochloride: a titrimetric method and another by HPLC using as mobile phase water pH 3.5: acetonitrile (65:35). These methods were applied to the determination of Fluoxetine as such or in formulations (capsules). The titrimetric method is an alternative for pharmacies and small industries. Both methods showed accuracy and precision and are an alternative to the official methods.

Non Destructive Analysis of Fsw Welds using Ultrasonic Signal Analysis

NASA Astrophysics Data System (ADS)

Pavan Kumar, T.; Prabhakar Reddy, P.

2017-08-01

Friction Stir Welding is an evolving metal joining technique and is mostly used in joining materials which cannot be easily joined by other available welding techniques. It is a technique which can be used for welding dissimilar materials also. The strength of the weld joint is determined by the way in which these material are mixing with each other, since we are not using any filler material for the welding process the intermixing has a significant importance. The complication with the friction stir welding process is that there are many process parameters which effect this intermixing process such as tool geometry, rotating speed of the tool, transverse speed etc., In this study an attempt is made to compare the material flow and weld quality of various weldments by changing the parameters. Ultrasonic signal Analysis is used to characterize the microstructure of the weldments. use of ultrasonic waves is a non destructive, accurate and fast way of characterization of microstructure. In this method the relationship between the ultrasonic measured parameters and microstructures are evaluated using background echo and backscattered signal process techniques. The ultrasonic velocity and attenuation measurements are dependent on the elastic modulus and any change in the microstructure is reflected in the ultrasonic velocity. An insight into material flow is essential to determine the quality of the weld. Hence an attempt is made in this study to know the relationship between tool geometry and the pattern of material flow and resulting weld quality the experiments are conducted to weld dissimilar aluminum alloys and the weldments are characterized using and ultra Sonic signal processing. Characterization is also done using Scanning Electron Microscopy. It is observed that there is a good correlation between the ultrasonic signal processing results and Scanning Electron Microscopy on the observed precipitates. Tensile tests and hardness tests are conducted on the

A Gaussian beam method for ultrasonic non-destructive evaluation modeling

NASA Astrophysics Data System (ADS)

Jacquet, O.; Leymarie, N.; Cassereau, D.

2018-05-01

The propagation of high-frequency ultrasonic body waves can be efficiently estimated with a semi-analytic Dynamic Ray Tracing approach using paraxial approximation. Although this asymptotic field estimation avoids the computational cost of numerical methods, it may encounter several limitations in reproducing identified highly interferential features. Nevertheless, some can be managed by allowing paraxial quantities to be complex-valued. This gives rise to localized solutions, known as paraxial Gaussian beams. Whereas their propagation and transmission/reflection laws are well-defined, the fact remains that the adopted complexification introduces additional initial conditions. While their choice is usually performed according to strategies specifically tailored to limited applications, a Gabor frame method has been implemented to indiscriminately initialize a reasonable number of paraxial Gaussian beams. Since this method can be applied for an usefully wide range of ultrasonic transducers, the typical case of the time-harmonic piston radiator is investigated. Compared to the commonly used Multi-Gaussian Beam model [1], a better agreement is obtained throughout the radiated field between the results of numerical integration (or analytical on-axis solution) and the resulting Gaussian beam superposition. Sparsity of the proposed solution is also discussed.

An extended data mining method for identifying differentially expressed assay-specific signatures in functional genomic studies.

PubMed

Rollins, Derrick K; Teh, Ailing

2010-12-17

Microarray data sets provide relative expression levels for thousands of genes for a small number, in comparison, of different experimental conditions called assays. Data mining techniques are used to extract specific information of genes as they relate to the assays. The multivariate statistical technique of principal component analysis (PCA) has proven useful in providing effective data mining methods. This article extends the PCA approach of Rollins et al. to the development of ranking genes of microarray data sets that express most differently between two biologically different grouping of assays. This method is evaluated on real and simulated data and compared to a current approach on the basis of false discovery rate (FDR) and statistical power (SP) which is the ability to correctly identify important genes. This work developed and evaluated two new test statistics based on PCA and compared them to a popular method that is not PCA based. Both test statistics were found to be effective as evaluated in three case studies: (i) exposing E. coli cells to two different ethanol levels; (ii) application of myostatin to two groups of mice; and (iii) a simulated data study derived from the properties of (ii). The proposed method (PM) effectively identified critical genes in these studies based on comparison with the current method (CM). The simulation study supports higher identification accuracy for PM over CM for both proposed test statistics when the gene variance is constant and for one of the test statistics when the gene variance is non-constant. PM compares quite favorably to CM in terms of lower FDR and much higher SP. Thus, PM can be quite effective in producing accurate signatures from large microarray data sets for differential expression between assays groups identified in a preliminary step of the PCA procedure and is, therefore, recommended for use in these applications.

Non-destructive NIR-FT-Raman spectroscopy of plant and animal tissues, of food and works of art.

PubMed

Schrader, B; Schulz, H; Andreev, G N; Klump, H H; Sawatzki, J

2000-10-02

Just after the discovery of Raman spectroscopy in 1928, it became evident that fluorescence with a quantum yield of several orders of magnitude higher than that of the Raman effect was a great and apparently unbeatable competitor. Raman spectroscopy could therefore, in spite of many exciting advantages during the last 60 years, not be applied as an analytical routine method: for nearly every sample, fluorescing impurities had to be removed by distillation or crystallisation. Purification, however, is not possible for cells and tissues, since the removal of the fluorescing enzymes and coenzymes would destroy the cells. There is fortunately one alternative solution. When excited with the radiation of the Nd:YAG laser at 1064 nm Raman spectra are practically free of fluorescence. Raman spectra can now be recorded with minimal sample preparation. In order to facilitate non-destructive Raman spectroscopy of any sample, cells and tissues, food, textiles and works of art, a new entrance optics for Raman spectrometers is used. Typical results from several fields are demonstrated.

Non Destructive Test Dye Penetrant and Ultrasonic on Welding SMAW Butt Joint with Acceptance Criteria ASME Standard

NASA Astrophysics Data System (ADS)

Endramawan, T.; Sifa, A.

2018-02-01

The purpose of this research is to know the type of discontinuity of SMAW welding result and to determine acceptance criteria based on American Society of Mechanical Engineer (ASME) standard. Material used is mild steel 98,71% Fe and 0,212% C with hardness 230 VHN with specimen diameter 20 cm and thickness 1.2 cm which is welded use SMAW butt joint with electrode for rooting LB 52U diameter 2.6 mm, current 70 Ampere and voltage 380 volt, filler used LB 5218 electrode diameter 3.2 mm with current 80 Ampere and 380 volt. The method used to analyze the welded with non destructive test dye penetrant (PT) method to see indication on the surface of the object and Ultrasonic (UT) to see indication on the sub and inner the surface of the object, the result is discontinuity recorded and analyzed and then the discontinuity is determine acceptance criteria based on the American Society of Mechanical Engineer (ASME) standards. The result show the discontinuity of porosity on the surface of the welded and inclusion on sub material used ultrasonic test, all indication on dye penetrant or ultrasonic test if there were rejected of result of welded that there must be gouging on part which rejected and then re-welding.

[Non-destructive detection research for hollow heart of potato based on semi-transmission hyperspectral imaging and SVM].

PubMed

Huang, Tao; Li, Xiao-yu; Xu, Meng-ling; Jin, Rui; Ku, Jing; Xu, Sen-miao; Wu, Zhen-zhong

2015-01-01

The quality of potato is directly related to their edible value and industrial value. Hollow heart of potato, as a physiological disease occurred inside the tuber, is difficult to be detected. This paper put forward a non-destructive detection method by using semi-transmission hyperspectral imaging with support vector machine (SVM) to detect hollow heart of potato. Compared to reflection and transmission hyperspectral image, semi-transmission hyperspectral image can get clearer image which contains the internal quality information of agricultural products. In this study, 224 potato samples (149 normal samples and 75 hollow samples) were selected as the research object, and semi-transmission hyperspectral image acquisition system was constructed to acquire the hyperspectral images (390-1 040 nn) of the potato samples, and then the average spectrum of region of interest were extracted for spectral characteristics analysis. Normalize was used to preprocess the original spectrum, and prediction model were developed based on SVM using all wave bands, the accurate recognition rate of test set is only 87. 5%. In order to simplify the model competitive.adaptive reweighed sampling algorithm (CARS) and successive projection algorithm (SPA) were utilized to select important variables from the all 520 spectral variables and 8 variables were selected (454, 601, 639, 664, 748, 827, 874 and 936 nm). 94. 64% of the accurate recognition rate of test set was obtained by using the 8 variables to develop SVM model. Parameter optimization algorithms, including artificial fish swarm algorithm (AFSA), genetic algorithm (GA) and grid search algorithm, were used to optimize the SVM model parameters: penalty parameter c and kernel parameter g. After comparative analysis, AFSA, a new bionic optimization algorithm based on the foraging behavior of fish swarm, was proved to get the optimal model parameter (c=10. 659 1, g=0. 349 7), and the recognition accuracy of 10% were obtained for the AFSA

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays

PubMed Central

Reverté, Laia; Soliño, Lucía; Carnicer, Olga; Diogène, Jorge; Campàs, Mònica

2014-01-01

The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of alternative or complementary methods providing functional or toxicological information may provide advantages in terms of risk identification, but also low cost, simplicity, ease of use and high-throughput analysis. This article gives an overview of the immunoassays, cell-based assays, receptor-binding assays and biosensors that have been developed for the screening and quantification of emerging marine toxins: palytoxins, ciguatoxins, cyclic imines and tetrodotoxins. Their advantages and limitations are discussed, as well as their possible integration in research and monitoring programs. PMID:25431968

Screening for anthracnose disease resistance in strawberry using a detached leaf assay

USDA-ARS?s Scientific Manuscript database

Inoculation of detached strawberry leaves with Colletotrichum species may provide a rapid, non-destructive method of identifying anthracnose resistant germplasm. The reliability and validity of assessing disease severity is critical to disease management decisions. We inoculated detached strawberr...

How to increase the efficiency of the electrical discharge method for destruction of nonconductive solid materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Voitenko, N. V., E-mail: tevn@hvd.tpu.ru; Yudin, A. S.; Kuznetsova, N. S.

The paper deals with the relevance of using electrical discharge technology for construction works in the city. The technical capabilities of the installation for the multi-borehole electro-discharge splitting off and destruction of rocks and concrete are described. The ways to increase the efficiency of the electrical discharge method for the destruction of solids are proposed. In order to augment the discharge circuit energy, the energy storage is separated into two individual capacitor batteries. The throttle with the inductance of 28.6 μH is installed in one of the batteries, which increases the duration of the channel energy release to 400 μsmore » and the efficiency of electrical discharge splitting off of concrete.« less

QUANTITATIVE NON-DESTRUCTIVE EVALUATION (QNDE) OF THE ELASTIC MODULI OF POROUS TIAL ALLOYS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yeheskel, O.

2008-02-28

The elastic moduli of {gamma}-TiA1 were studied in porous samples consolidated by various techniques e.g. cold isostatic pressing (CIP), pressure-less sintering, or hot isostatic pressing (HIP). Porosity linearly affects the dynamic elastic moduli of samples. The results indicate that the sound wave velocities and the elastic moduli affected by the processing route and depend not only on the attained density but also on the consolidation temperature. In this paper we show that there is linear correlation between the shear and the longitudinal sound velocities in porous TiA1. This opens the way to use a single sound velocity as a toolmore » for quantitative non-destructive evaluation (QNDE) of porous TiA1 alloys. Here we demonstrate the applicability of an equation derived from the elastic theory and used previously for porous cubic metals.« less

Performance of human fecal anaerobe-associated PCR-based assays in a multi-laboratory method evaluation study

USGS Publications Warehouse

Layton, Blythe A.; Cao, Yiping; Ebentier, Darcy L.; Hanley, Kaitlyn; Ballesté, Elisenda; Brandão, João; Byappanahalli, Muruleedhara N.; Converse, Reagan; Farnleitner, Andreas H.; Gentry-Shields, Jennifer; Gourmelon, Michèle; Lee, Chang Soo; Lee, Jiyoung; Lozach, Solen; Madi, Tania; Meijer, Wim G.; Noble, Rachel; Peed, Lindsay; Reischer, Georg H.; Rodrigues, Raquel; Rose, Joan B.; Schriewer, Alexander; Sinigalliano, Chris; Srinivasan, Sangeetha; Stewart, Jill; ,; Laurie, C.; Wang, Dan; Whitman, Richard; Wuertz, Stefan; Jay, Jenny; Holden, Patricia A.; Boehm, Alexandria B.; Shanks, Orin; Griffith, John F.

2013-01-01

A number of PCR-based methods for detecting human fecal material in environmental waters have been developed over the past decade, but these methods have rarely received independent comparative testing in large multi-laboratory studies. Here, we evaluated ten of these methods (BacH, BacHum-UCD, Bacteroides thetaiotaomicron (BtH), BsteriF1, gyrB, HF183 endpoint, HF183 SYBR, HF183 Taqman®, HumM2, and Methanobrevibacter smithii nifH (Mnif)) using 64 blind samples prepared in one laboratory. The blind samples contained either one or two fecal sources from human, wastewater or non-human sources. The assay results were assessed for presence/absence of the human markers and also quantitatively while varying the following: 1) classification of samples that were detected but not quantifiable (DNQ) as positive or negative; 2) reference fecal sample concentration unit of measure (such as culturable indicator bacteria, wet mass, total DNA, etc); and 3) human fecal source type (stool, sewage or septage). Assay performance using presence/absence metrics was found to depend on the classification of DNQ samples. The assays that performed best quantitatively varied based on the fecal concentration unit of measure and laboratory protocol. All methods were consistently more sensitive to human stools compared to sewage or septage in both the presence/absence and quantitative analysis. Overall, HF183 Taqman® was found to be the most effective marker of human fecal contamination in this California-based study.

Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays

PubMed Central

Breveglieri, Giulia; Travan, Anna; D’Aversa, Elisabetta; Cosenza, Lucia Carmela; Pellegatti, Patrizia; Guerra, Giovanni; Gambari, Roberto

2017-01-01

The β-thalassemias are genetic disorder caused by more than 200 mutations in the β-globin gene, resulting in a total (β0) or partial (β+) deficit of the globin chain synthesis. The most frequent Mediterranean mutations for β-thalassemia are: β039, β+IVSI-110, β+IVSI-6 and β0IVSI-1. Several molecular techniques for the detection of point mutations have been developed based on the amplification of the DNA target by polymerase chain reaction (PCR), but they could be labor-intensive and technically demanding. On the contrary, TaqMan® genotyping assays are a simple, sensitive and versatile method suitable for the single nucleotide polymorphism (SNP) genotyping affecting the human β-globin gene. Four TaqMan® genotyping assays for the most common β-thalassemia mutations present in the Mediterranean area were designed and validated for the genotype characterization of genomic DNA extracted from 94 subjects comprising 25 healthy donors, 33 healthy carriers and 36 β-thalassemia patients. In addition, 15 specimens at late gestation (21–39 gestational weeks) and 11 at early gestation (5–18 gestational weeks) were collected from pregnant women, and circulating cell-free fetal DNAs were extracted and analyzed with these four genotyping assays. We developed four simple, inexpensive and versatile genotyping assays for the postnatal and prenatal identification of the thalassemia mutations β039, β+IVSI-110, β+IVSI-6, β0IVSI-1. These genotyping assays are able to detect paternally inherited point mutations in the fetus and could be efficiently employed for non-invasive prenatal diagnosis of β-globin gene mutations, starting from the 9th gestational week. PMID:28235086

Non-Destructive Inspection of Impact Damage in Composite Aircraft Panels by Ultrasonic Guided Waves and Statistical Processing.

PubMed

Capriotti, Margherita; Kim, Hyungsuk E; Scalea, Francesco Lanza di; Kim, Hyonny

2017-06-04

This paper discusses a non-destructive evaluation (NDE) technique for the detection of damage in composite aircraft structures following high energy wide area blunt impact (HEWABI) from ground service equipment (GSE), such as heavy cargo loaders and other heavy equipment. The test structures typically include skin, co-cured stringers, and C-frames that are bolt-connected onto the skin with shear ties. The inspection exploits the waveguide geometry of these structures by utilizing ultrasonic guided waves and a line scan approach. Both a contact prototype and a non-contact prototype were developed and tested on realistic test panels subjected to impact in the laboratory. The results are presented in terms of receiver operating characteristic curves that show excellent probability of detection with low false alarm rates for defects located in the panel skin and stringers.

[Efficiency of combined methods of hemorroid treatment using hal-rar and laser destruction].

PubMed

Rodoman, G V; Kornev, L V; Shalaeva, T I; Malushenko, R N

2017-01-01

To develop the combined method of treatment of hemorrhoids with arterial ligation under Doppler control and laser destruction of internal and external hemorrhoids. The study included 100 patients with chronic hemorrhoids stage II and III. Combined method of HAL-laser was used in study group, HAL RAR-technique in control group 1 and closed hemorrhoidectomy with linear stapler in control group 2. Сomparative evaluation of results in both groups was performed. Combined method overcomes the drawbacks of traditional surgical treatment and limitations in external components elimination which are inherent for HAL-RAR. Moreover, it has a higher efficiency in treating of hemorrhoids stage II-III compared with HAL-RAR and is equally safe and well tolerable for patients. This method does not increase the risk of recurrence, reduces incidence of complications and time of disability.

Method Modification Study for the Thermo Scientific SureTect™ Listeria Species Assay-Matrix Extension.

PubMed

Cloke, Jonathan; Evans, Katharine; Crabtree, David; Hughes, Annette; Simpson, Helen; Holopainen, Jani; Wickstrand, Nina; Kauppinen, Mikko; Leon-Velarde, Carlos; Larson, Nathan; Dave, Keron; Chen, Yi; Ryser, Elliot; Carter, Mark

2016-01-01

The Thermo Scientific™ SureTect™ Listeria species assay is a new real-time PCR assay for the detection of all species of Listeria in food and environmental samples. The assay was originally certified as Performance Tested Methods(SM) (PTM) 071304 in 2013. This report details the method modification study undertaken to extend the performance claims of the assay for matrixes of raw ground turkey, raw ground pork, bagged lettuce, raw pork sausages, pasteurized 2% fat milk, raw cod, pasteurized brie cheese, and ice cream. The method modification study was conducted using the AOAC Research Institute (RI) PTM program to validate the SureTect PCR assay in comparison to the reference method detailed in ISO 11290-1:1996 including amendment 1:2004. All matrixes were tested by Thermo Fisher Scientific (Basingstoke, United Kingdom). In addition, three matrixes (raw cod, bagged lettuce, and pasteurized brie cheese) were analyzed independently as part of the AOAC RI-controlled independent laboratory study by the University of Guelph, Canada. Using probability of detection statistical analysis, there was no significant difference in the performance between the SureTect assay and the International Organization for Standardization reference method for any of the matrixes analyzed in this study.

Non-Destructive Evaluation of Kissing Bonds using Local Defect Resonance (LDR) Spectroscopy: A Simulation Study

NASA Astrophysics Data System (ADS)

Delrue, S.; Tabatabaeipour, M.; Hettler, J.; Van Den Abeele, K.

With the growing demand from industry to optimize and further develop existing Non-Destructive Testing & Evaluation (NDT&E) techniques or new methods to detect and characterize incipient damage with high sensitivity and increased quality, ample efforts have been devoted to better understand the typical behavior of kissing bonds, such as delaminations and cracks. Recently, it has been shown experimentally that the nonlinear ultrasonic response of kissing bonds could be enhanced by using Local Defect Resonance (LDR) spectroscopy. LDR spectroscopy is an efficient NDT technique that takes advantage of the characteristic fre- quencies of the defect (defect resonances) in order to provide maximum acoustic wave-defect interaction. In fact, for nonlinear methodologies, the ultrasonic excitation of the sample should occur at either multiples or integer ratios of the characteristic defect resonance frequencies, in order to obtain the highest signal-to-noise response in the nonlinear LDR spectroscopy. In this paper, the potential of using LDR spectroscopy for the detection, localization and characterization of kissing bonds is illustrated using a 3D simulation code for elastic wave propagation in materials containing closed but dynamically active cracks or delaminations. Using the model, we are able to define an appropriate method, based on the Scaling Subtraction Method (SSM), to determine the local defect resonance frequencies of a delamination in a composite plate and to illustrate an increase in defect nonlinearity due to LDR. The simulation results will help us to obtain a better understanding of the concept of LDR and to assist in the further design and testing of LDR spectroscopy for the detection, localization and characterization of kissing bonds.

Annual Report on the Activities and Publications of the DHS-DNDO-NTNFC Sponsored Post-doctoral Fellow at Los Alamos National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rim, Jung Ho; Tandon, Lav

This report is a summary of the projects Jung Rim is working on as a DHS postdoctoral fellow at Los Alamos National Laboratory. These research projects are designed to explore different radioanalytical methods to support nuclear forensics applications. The current projects discussed here include development of alpha spectroscopy method for 240/239Pu Isotopic ratio measurement, non-destructive uranium assay method using gamma spectroscopy, and 236U non-destructive uranium analysis using FRAM code. This report documents the work that has been performed since the start of the postdoctoral appointment.

Non-Destructive and Discriminating Identification of Illegal Drugs by Transient Absorption Spectroscopy in the Visible and Near-IR Wavelength Range

NASA Astrophysics Data System (ADS)

Sato, Chie; Furube, Akihiro; Katoh, Ryuzi; Nonaka, Hidehiko; Inoue, Hiroyuki

2008-11-01

We have tested the possibility of identifying illegal drugs by means of nanosecond transient absorption spectroscopy with a 10-ns UV-laser pulse for the excitation light and visible-to-near-IR light for the probe light. We measured the transient absorption spectra of acetonitrile solutions of d-methamphetamine, dl-3,4-methylenedioxymethamphetamine hydrochloride (MDMA), and dl-N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine hydrochloride (MBDB), which are illegal drugs widely consumed in Japan. Transient absorption signals of these drugs were observed between 400 and 950 nm, a range in which they are transparent in the ground state. By analyzing the spectra in terms of exponential and Gaussian functions, we could identify the drugs and discriminate them from chemical substances having similar structures. We propose that transient absorption spectroscopy will be a useful, non-destructive method of inspecting for illegal drugs, especially when they are dissolved in liquids. Such a method may even be used for drugs packed in opaque materials if it is further extended to utilize intense femtosecond laser pulses.

Analysis of non-destructive current simulators of flux compression generators.

PubMed

O'Connor, K A; Curry, R D

2014-06-01

Development and evaluation of power conditioning systems and high power microwave components often used with flux compression generators (FCGs) requires repeated testing and characterization. In an effort to minimize the cost and time required for testing with explosive generators, non-destructive simulators of an FCG's output current have been developed. Flux compression generators and simulators of FCGs are unique pulsed power sources in that the current waveform exhibits a quasi-exponential increasing rate at which the current rises. Accurately reproducing the quasi-exponential current waveform of a FCG can be important in designing electroexplosive opening switches and other power conditioning components that are dependent on the integral of current action and the rate of energy dissipation. Three versions of FCG simulators have been developed that include an inductive network with decreasing impedance in time. A primary difference between these simulators is the voltage source driving them. It is shown that a capacitor-inductor-capacitor network driving a constant or decreasing inductive load can produce the desired high-order derivatives of the load current to replicate a quasi-exponential waveform. The operation of the FCG simulators is reviewed and described mathematically for the first time to aid in the design of new simulators. Experimental and calculated results of two recent simulators are reported with recommendations for future designs.

Non-Destructive Optical Monitoring of Grape Maturation by Proximal Sensing

PubMed Central

Ben Ghozlen, Naïma; Cerovic, Zoran G.; Germain, Claire; Toutain, Sandrine; Latouche, Gwendal

2010-01-01

A new, commercial, fluorescence-based optical sensor for plant constituent assessment was recently introduced. This sensor, called the Multiplex® (FORCE-A, Orsay, France), was used to monitor grape maturation by specifically monitoring anthocyanin accumulation. We derived the empirical anthocyanin content calibration curves for Champagne red grape cultivars, and we also propose a general model for the influence of the proportion of red berries, skin anthocyanin content and berry size on Multiplex® indices. The Multiplex® was used on both berry samples in the laboratory and on intact clusters in the vineyard. We found that the inverted and log-transformed far-red fluorescence signal called the FERARI index, although sensitive to sample size and distance, is potentially the most widely applicable. The more robust indices, based on chlorophyll fluorescence excitation ratios, showed three ranges of dependence on anthocyanin content. We found that up to 0.16 mg cm−2, equivalent to approximately 0.6 mg g−1, all indices increase with accumulation of skin anthocyanin content. Excitation ratio-based indices decrease with anthocyanin accumulation beyond 0.27 mg cm−2. We showed that the Multiplex® can be advantageously used in vineyards on intact clusters for the non-destructive assessment of anthocyanin content of vine blocks and can now be tested on other fruits and vegetables based on the same model. PMID:22163456

Use of the Filovirus Animal Non-Clinical Group (FANG) Ebola virus immuno-assay requires fewer study participants to power a study than the Alpha Diagnostic International assay

PubMed Central

Logue, James; Tuznik, Kaylie; Follmann, Dean; Grandits, Greg; Marchand, Jonathan; Reilly, Cavan; Sarro, Yeya dit Sadio; Pettitt, James; Stavale, Eric J.; Fallah, Mosoka; Olinger, Gene G.; Bolay, Fatorma K.; Hensley, Lisa E.

2018-01-01

As part of the scientific community’s development of medical countermeasures against Ebola virus disease, optimization of standardized assays for product evaluation is paramount. The recent outbreak heightened awareness to the scarcity of available assays and limited information on performance and reproducibility. To evaluate the immunogenicity of vaccines entering Phase I–III trials and to identify survivors, two enzyme-linked immunosorbent assays, the Filovirus Animal Non-Clinical Group assay and the Alpha Diagnostics International assay, were evaluated for detection of immunoglobulin G against Ebola virus glycoprotein. We found that the Filovirus Animal Nonclinical Group assay produced a wider range of relative antibody concentrations, higher assay precision, larger relative accuracy range, and lower regional background. Additionally, to sufficiently power a vaccine trial, use of the Filovirus Animal Nonclinical Group assay would require one third the number of participants than the Alpha Diagnostics International assay. This reduction in needed study participants will require less money, fewer man hours, and much less time to evaluate vaccine immunogenicity. PMID:29481881

Advanced Laser-Compton Gamma-Ray Sources for Nuclear Materials Detection, Assay and Imaging

NASA Astrophysics Data System (ADS)

Barty, C. P. J.

2015-10-01

Highly-collimated, polarized, mono-energetic beams of tunable gamma-rays may be created via the optimized Compton scattering of pulsed lasers off of ultra-bright, relativistic electron beams. Above 2 MeV, the peak brilliance of such sources can exceed that of the world's largest synchrotrons by more than 15 orders of magnitude and can enable for the first time the efficient pursuit of nuclear science and applications with photon beams, i.e. Nuclear Photonics. Potential applications are numerous and include isotope-specific nuclear materials management, element-specific medical radiography and radiology, non-destructive, isotope-specific, material assay and imaging, precision spectroscopy of nuclear resonances and photon-induced fission. This review covers activities at the Lawrence Livermore National Laboratory related to the design and optimization of mono-energetic, laser-Compton gamma-ray systems and introduces isotope-specific nuclear materials detection and assay applications enabled by them.

Non-destructive inspection using HTS SQUID on aluminum liner covered by CFRP

NASA Astrophysics Data System (ADS)

Hatsukade, Y.; Yotsugi, K.; Sakaguchi, Y.; Tanaka, S.

2007-10-01

An eddy-current-based SQUID non-destructive inspection (NDI) system to detect deep-lying cracks in multi-layer composite-Al vessels was developed taking advantage of the uncontested sensitivity of HTS-SQUID in low-frequency range. An HTS-SQUID gradiometer was mounted in a pulse tube cryocooler. A pair of differential coils with C-shaped ferrite cores was employed to induce an enhanced eddy current in an Al vessel wrapped in a carbon fiber reinforced plastic (CFRP) cover. Ellipsoidal dome-shaped Al liners containing through cracks, which were made by pressure cycle tests, in the CFRP covers with total thickness of 6 mm (CFPR 3 mm, and Al 3 mm) were inspected by the system. While inducing eddy currents in the vessels with excitation fields at 100 Hz or 7 kHz, the vessels were rotated under the HTS-SQUID. Above the cracks, anomalous signals due to the cracks were clearly detected at both frequencies. These results suggested the SQUID-NDI technique would be a possible candidate for inspection of high-pressure multi-layer composite-Al vessels.

Molecular Method Confirms Canine Leishmania Infection Detected by Serological Methods in Non-Endemic Area of Brazil

PubMed Central

Riboldi, Emeline; Carvalho, Flavio; Romão, Pedro Roosevelt Torres; Barcellos, Regina Bones; Bello, Graziele Lima; Ramos, Raquel Rocha; de Oliveira, Rosemari Terezinha; Júnior, João Pessoa Araújo; Rossetti, Maria Lucia; Dallegrave, Eliane

2018-01-01

In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n=107), São Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic (DPP®) and ELISA EIE® assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in São Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas. PMID:29529845

Immunoenzyme assay of nonylphenol: study of selectivity and detection of alkylphenolic non-ionic surfactants in water samples.

PubMed

Mart'ianov, Andrey A; Dzantiev, Boris B; Zherdev, Anatoly V; Eremin, Sergei A; Cespedes, Raquel; Petrovic, Mira; Barcelo, Damia

2005-01-30

Immunoenzyme assay (ELISA) is proposed and characterized for determination of alkylphenol ethoxylates, a primary class of manufactured non-ionic surfactants. The assay is based on the obtained polyclonal antibodies against nonylphenol (NP), the main stable intermediate of the decomposition of nonylphenol ethoxylates. A mixture of non-modified branched isomers of NP was applied as hapten coupled to protein carriers by Mannich reaction with the use of formaldehyde. The proposed ELISA format is based on immobilized NP-(soybean trypsin inhibitor) conjugate as a competitor of antigen molecules contained in the tested sample for binding with specific antibodies indirectly labeled via an anti-species immunoperoxidase conjugate. The developed ELISA allows to reveal NP with the limit of detection about 10ngml(-1) and NP-related compounds such as octylphenol, alkylphenoletoxylates, alkylphenolcarboxylates and their halogenated derivatives. The ELISA was applied for assaying polluted water samples, namely influents and effluents from different wastewater treatment plants (WWTP) and tap water. ELISA and chromatographic data demonstrate good correlation (r = 0.94), while ELISA gives higher values. Due to endocrine disrupting and other toxic activities of some metabolites of alkylphenolic non-ionic surfactants, the developed assay may be effectively used in ecological monitoring and sanitary control.

Brief Report: A mass spectrometry assay to simultaneously analyze ROS1 and RET fusion gene expression in non-small cell lung cancer

PubMed Central

Wijesinghe, Priyanga; Bepler, Gerold

2014-01-01

Introduction ROS1 and RET gene fusions were recently discovered in non-small cell lung cancer (NSCLC) as potential therapeutic targets with small molecule kinase inhibitors. The conventional screening methods of these fusions are time consuming and require samples of high quality and quantity. Here, we describe a novel and efficient method by coupling the power of multiplexing PCR and the sensitivity of mass spectrometry. Methods The multiplex mass spectrometry platform simultaneously tests samples for the expression of nine ROS1 and six RET fusion genes. The assay incorporates detection of wild-type exon junctions immediately upstream and downstream of the fusion junction to exclude false negative results. To flag false positives, the system also comprises two independent assays for each fusion gene junction. Results The characteristic mass spectrometric peaks of the gene fusions were obtained using engineered plasmid constructs. Specific assays targeting the wild-type gene exon junctions were validated using cDNA from lung tissue of healthy individuals. The system was further validated using cDNA derived from NSCLC cell lines that express endogenous fusion genes. The expressed ROS1-SLC34A2 and CCDC6-RET gene fusions from the NSCLC cell lines HCC78 and LC-2/ad, respectively, were accurately detected by the novel assay. The assay is extremely sensitive, capable of detecting an event in test specimens containing 0.5% positive tumors. Conclusion The novel multiplexed assay is robustly capable of detecting 15 different clinically relevant RET and ROS1 fusion variants. The benefits of this detection method include exceptionally low sample input, high cost efficiency, flexibility, and rapid turnover. PMID:25384172

Destructive examination of shipping package 9975-02644

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daugherty, W. L.

Destructive and non-destructive examinations have been performed on the components of shipping package 9975-02644 as part of a comprehensive SRS surveillance program for plutonium material stored in the K-Area Complex (KAC). During the field surveillance inspection of this package in KAC, three non-conforming conditions were noted: the axial gap of 1.389 inch exceeded the 1 inch maximum criterion, the exposed height of the lead shield was greater than the 4.65 inch maximum criterion, and the difference between the upper assembly inside height and the exposed height of the lead shield was less than the 0.425 inch minimum criterion. All threemore » of these observations relate to axial shrinkage of the lower fiberboard assembly. In addition, liquid water (condensation) was observed on the interior of the drum lid, the thermal blanket and the air shield.« less

Interpretation of actinide-distribution data obtained from non-destructive and destructive post-test analyses of an intact-core column of Culebra dolomite.

PubMed

Perkins, W G; Lucero, D A

2001-02-01

The US Department of Energy (DOE), with technical assistance from Sandia National Laboratories, has successfully received EPA certification and opened the Waste Isolation Pilot Plant (WIPP), a nuclear waste disposal facility located approximately 42 km east of Carlsbad, NM. Performance assessment (PA) analyses indicate that human intrusions by inadvertent, intermittent drilling for resources provide the only credible mechanisms for significant releases of radionuclides from the disposal system. For long-term brine releases, migration pathways through the permeable layers of rock above the Salado formation are important. Major emphasis is placed on the Culebra Member of the Rustler Formation because this is the most transmissive geologic layer overlying the WIPP site. In order to help quantify parameters for the calculated releases, radionuclide transport experiments have been carried out using intact-core columns obtained from the Culebra dolomite member of the Rustler Formation within the WIPP site. This paper deals primarily with results of analyses for 241Pu and 241Am distributions developed during transport experiments in one of these cores. Transport experiments were done using a synthetic brine that simulates Culebra brine at the core recovery location (the WIPP air-intake shaft (AIS)). Hydraulic characteristics (i.e., apparent porosity and apparent dispersion coefficient) for intact-core columns were obtained via experiments using the conservative tracer 22Na. Elution experiments carried out over periods of a few days with tracers 232U and 239Np indicated that these tracers were weakly retarded as indicated by delayed elution of the species. Elution experiments with tracers 241Pu and 241Am were attempted but no elution of either species has been observed to date, including experiments of many months' duration. In order to quantify retardation of the non-eluted species 241Pu and 241Am after a period of brine flow, non-destructive and destructive analyses of

Lactase Non-Persistence Genotyping: Comparison of Two Real-Time PCR Assays and Assessment of Concomitant Fructose/Sorbitol Malabsorption Rates.

PubMed

Enko, Dietmar; Pollheimer, Verena; Németh, Stefan; Pühringer, Helene; Stolba, Robert; Halwachs-Baumann, Gabriele; Kriegshäuser, Gernot

2016-01-01

Genetic testing is a standard technique for the diagnosis of primary adult-type hypolactasia, also referred to as lactase non-persistence. The aim of this study was to compare the lactase gene (LCT) C/T-13910 polymorphism genotyping results of two commercially available real-time (RT)-PCR assays in patients referred to our outpatient clinic for primary lactose malabsorption testing. Furthermore, concomitant conditions of fructose/sorbitol malabsorption were assessed. Samples obtained from 100 patients were tested in parallel using the LCT T-13910C ToolSet for Light Cycler (Roche, Rotkreuz, Switzerland) and the LCT-13910C>T RealFast Assay (ViennaLab Diagnostics GmbH, Vienna, Austria). Additionally, patients were also screened for the presence of fructose/sorbitol malabsorption by functional hydrogen (H2)/methane (CH4) breath testing (HMBT). Cohen's Kappa (κ) was used to calculate the agreement between the two genotyping methods. The exact Chi-Square test was performed to compare fructose/sorbitol HMBT with LCT genotyping results. Twenty-one (21.0%) patients had a LCT C/C-13910 genotype suggestive of lactase non-persistence, and 79 (79.0%) patients were identified with either a LCT T/C-13910 or T/T-13910 genotype (i.e., lactase persistence). In all genotype groups, concordance between the two RT-PCR assays was 100%. Cohen's κ demonstrated perfect observed agreement (p < 0.001, κ = 1). Fructose and sorbitol malabsorption was observed in 13/100 (13.0%) and 25/100 (25.0%) individuals, respectively. Both RT-PCR assays are robust and reliable LCT genotyping tools in a routine clinical setting. Concomitant fructose and/or sorbitol malabsorption should be considered in individuals with suspected lactase-non-persistence. However, standardization of clinical interpretation of laboratory HMBT results is required.

The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis

PubMed Central

Schwark, Thorsten; Meyer, Patrick; Harder, Melanie; Modrow, Jan-Hendrick; von Wurmb-Schwark, Nicole

2012-01-01

Objective Short tandem repeat (STR) analysis using commercial multiplex PCR kits is the method of choice for kinship testing and trace analysis. However, under certain circumstances (deficiency testing, mutations, minute DNA amounts), STRs alone may not suffice. Methods We present a 50-plex single nucleotide polymorphism (SNP) assay based on the SNPs chosen by the SNPforID consortium as an additional method for paternity and for trace analysis. The new assay was applied to selected routine paternity and trace cases from our laboratory. Results and Conclusions Our investigation shows that the new SNP multiplex assay is a valuable method to supplement STR analysis, and is a powerful means to solve complicated genetic analyses. PMID:22851934

AlphaScreen-based homogeneous assay using a pair of 25-residue artificial proteins for high-throughput analysis of non-native IgG.

PubMed

Senga, Yukako; Imamura, Hiroshi; Miyafusa, Takamitsu; Watanabe, Hideki; Honda, Shinya

2017-09-29

Therapeutic IgG becomes unstable under various stresses in the manufacturing process. The resulting non-native IgG molecules tend to associate with each other and form aggregates. Because such aggregates not only decrease the pharmacological effect but also become a potential risk factor for immunogenicity, rapid analysis of aggregation is required for quality control of therapeutic IgG. In this study, we developed a homogeneous assay using AlphaScreen and AF.2A1. AF.2A1 is a 25-residue artificial protein that binds specifically to non-native IgG generated under chemical and physical stresses. This assay is performed in a short period of time. Our results show that AF.2A1-AlphaScreen may be used to evaluate the various types of IgG, as AF.2A1 recognizes the non-native structure in the constant region (Fc region) of IgG. The assay was effective for detection of non-native IgG, with particle size up to ca. 500 nm, generated under acid, heat, and stirring conditions. In addition, this technique is suitable for analyzing non-native IgG in CHO cell culture supernatant and mixed with large amounts of native IgG. These results indicate the potential of AF.2A1-AlphaScreen to be used as a high-throughput evaluation method for process monitoring as well as quality testing in the manufacturing of therapeutic IgG.

Non-destructive mobile monitoring of microbial contaminations on meat surfaces using porphyrin fluorescence intensities.

PubMed

Durek, J; Fröhling, A; Bolling, J; Thomasius, R; Durek, P; Schlüter, O K

2016-05-01

A non-destructive mobile system for meat quality monitoring was developed and investigated for the possible application along the whole production chain of fresh meat. Pork and lamb meat was stored at 5 °C for up to 20 days post mortem and measured with a fluorescence spectrometer. Additionally, the bacterial influence on the fluorescence signals was evaluated by different experimental procedures. Fluorescence of NADH and different porphyrins could be correlated to the growth of diverse bacteria and hence used for contamination monitoring. The increase of porphyrin fluorescence started after 9 days p.m. for pork and after 2 days p.m. for lamb meat. Based on the results, a mobile fluorescence system was built and compared with the laboratory system. The corrected function of the meat slices showed a root mean square error of 1156.97 r.u. and a mean absolute percentage error of 12.59%; for lamb the values were 470.81 r.u. and 15.55%, respectively. A mobile and non-invasive measurement system would improve the microbial security of fresh meat. Copyright © 2016 Elsevier Ltd. All rights reserved.

Non-destructive prediction of 'Hass' avocado dry matter via FT-NIR spectroscopy.

PubMed

Wedding, Brett B; White, Ronald D; Grauf, Steve; Wright, Carole; Tilse, Bonnie; Hofman, Peter; Gadek, Paul A

2011-01-30

The inability to consistently guarantee internal quality of horticulture produce is of major importance to the primary producer, marketers and ultimately the consumer. Currently, commercial avocado maturity estimation is based on the destructive assessment of percentage dry matter (%DM), and sometimes percentage oil, both of which are highly correlated with maturity. In this study the utility of Fourier transform (FT) near-infrared spectroscopy (NIRS) was investigated for the first time as a non-invasive technique for estimating %DM of whole intact 'Hass' avocado fruit. Partial least squares regression models were developed from the diffuse reflectance spectra to predict %DM, taking into account effects of intra-seasonal variation and orchard conditions. It was found that combining three harvests (early, mid and late) from a single farm in the major production district of central Queensland yielded a predictive model for %DM with a coefficient of determination for the validation set of 0.76 and a root mean square error of prediction of 1.53% for DM in the range 19.4-34.2%. The results of the study indicate the potential of FT-NIRS in diffuse reflectance mode to non-invasively predict %DM of whole 'Hass' avocado fruit. When the FT-NIRS system was assessed on whole avocados, the results compared favourably against data from other NIRS systems identified in the literature that have been used in research applications on avocados. 2010 Society of Chemical Industry.

Detection of Streptococcus pyogenes using rapid visual molecular assay.

PubMed

Zhao, Xiangna; He, Xiaoming; Li, Huan; Zhao, Jiangtao; Huang, Simo; Liu, Wei; Wei, Xiao; Ding, Yiwei; Wang, Zhaoyan; Zou, Dayang; Wang, Xuesong; Dong, Derong; Yang, Zhan; Yan, Xiabei; Huang, Liuyu; Du, Shuangkui; Yuan, Jing

2015-09-01

Streptococcus pyogenes is an increasingly important pathogen in many parts of the world. Rapid and accurate detection of S. pyogenes aids in the control of the infection. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and validated for the specific detection of S. pyogenes. The assay incorporates two methods: a chromogenic analysis using a calcein/Mn(2+) complex and real-time turbidity monitoring to assess the reaction. Both methods detected the target DNA within 60 min under 64°C isothermal conditions. The assay used specifically designed primers to target spy1258, and correctly identified 111 strains of S. pyogenes and 32 non-S. pyogenes strains, including other species of the genus Streptococcus. Tests using reference strains showed that the LAMP assay was highly specific. The sensitivity of the assay, with a detection limit of 1.49 pg DNA, was 10-fold greater than that of PCR. The LAMP assay established in this study is simple, fast and sensitive, and does not rely upon any special equipment; thus, it could be employed in clinical diagnosis. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Non-Destructive Inspection of Impact Damage in Composite Aircraft Panels by Ultrasonic Guided Waves and Statistical Processing

PubMed Central

Capriotti, Margherita; Kim, Hyungsuk E.; Lanza di Scalea, Francesco; Kim, Hyonny

2017-01-01

This paper discusses a non-destructive evaluation (NDE) technique for the detection of damage in composite aircraft structures following high energy wide area blunt impact (HEWABI) from ground service equipment (GSE), such as heavy cargo loaders and other heavy equipment. The test structures typically include skin, co-cured stringers, and C-frames that are bolt-connected onto the skin with shear ties. The inspection exploits the waveguide geometry of these structures by utilizing ultrasonic guided waves and a line scan approach. Both a contact prototype and a non-contact prototype were developed and tested on realistic test panels subjected to impact in the laboratory. The results are presented in terms of receiver operating characteristic curves that show excellent probability of detection with low false alarm rates for defects located in the panel skin and stringers. PMID:28772976

An experimental study of non-destructive testing on glass fibre reinforced polymer composites after high velocity impact event

NASA Astrophysics Data System (ADS)

Razali, N.; Sultan, M. T. H.; Cardona, F.

2016-10-01

A non-destructive testing method on Glass Fibre Reinforced Polymer (GFRP) after high velocity impact event using single stage gas gun (SSGG) is presented. Specimens of C- type and E-type fibreglass reinforcement, which were fabricated with 6mm, 8mm, 10mm and 12mm thicknesses and size 100 mm x 100 mm, were subjected to a high velocity impact with three types of bullets: conical, hemispherical and blunt at various gas gun pressure levels from 6 bar to 60 bar. Visual observation techniques using a lab microscope were used to determine the infringed damage by looking at the crack zone. Dye penetrants were used to inspect the area of damage, and to evaluate internal and external damages on the specimens after impact. The results from visual analysis of the impacted test laminates were discussed and presented. It was found that the impact damage started with induced delamination, fibre cracking and then failure, simultaneously with matrix cracking and breakage, and finally followed by the fibres pulled out. C-type experienced more damaged areas compared to E-type of GFRP.

Non-destructive characterization of oriental porcelain glazes and blue underglaze pigments using μ-EDXRF, μ-Raman and VP-SEM

NASA Astrophysics Data System (ADS)

Coutinho, M. L.; Muralha, V. S. F.; Mirão, J.; Veiga, J. P.

2014-03-01

The study of ancient materials with recognized cultural and economic value is a challenge to scientists and conservators, since it is usually necessary an approach through non-destructive techniques. Difficulties in establishing a correct analytical strategy are often significantly increased by the lack of knowledge on manufacture technologies and raw materials employed combined with the diversity of decay processes that may have acted during the lifetime of the cultural artefacts. A non-destructive characterization was performed on the glaze and underglaze pigments from a group of Chinese porcelain shards dated from the late Ming Dynasty (1368-1644) excavated at the Monastery of Santa Clara- a- Velha in Coimbra (Portugal). Chemical analysis was performed using micro-energy dispersive X-ray fluorescence spectrometry (μ-EDXRF). Mineralogical characterization was achieved by Raman microscopy (μ-Raman) and observation of small-surface crystallization dark spots with a metallic lustre in areas with high pigment concentration was done by variable pressure scanning electron microscopy (VP-SEM). Cobalt aluminate was identified as the blue underglaze pigment and a comparison of blue and dark blue pigments was performed by the ratio of Co, Mn, and Fe oxides, indicating a compositional difference between the two blue tonalities. Manganese oxide compounds were also identified as colouring agents in dark blue areas and surface migration of manganese compounds was verified.

Non-destructive electromechanical assessment (Arthro-BST) of human articular cartilage correlates with histological scores and biomechanical properties.

PubMed

Sim, S; Chevrier, A; Garon, M; Quenneville, E; Yaroshinsky, A; Hoemann, C D; Buschmann, M D

2014-11-01

The hand-held Arthro-BST™ device is used to map electromechanical properties of articular cartilage. The purpose of the study was to evaluate correlation of electromechanical properties with histological, biochemical and biomechanical properties of cartilage. Electromechanical properties (quantitative parameter (QP)) of eight human distal femurs were mapped manually ex vivo using the Arthro-BST (1 measure/site, 5 s/measure, 3209 sites). Osteochondral cores were then harvested from different areas on the femurs and assessed with the Mankin histological score. Prior to histoprocessing, cores were tested in unconfined compression. A subset of the cores was analyzed with polarized light microscopy (PLM) to assess collagen structure. Biochemical assays were done on additional cores to obtain water content and glycosaminoglycan (GAG) content. The QP corresponding to each core was calculated by averaging all QPs collected within 6 mm of the core center. The electromechanical QP correlated strongly with both the Mankin score and the PLM score (r = 0.73, P < 0.0001 and r = -0.70, P < 0.0001 respectively) thus accurately reflecting tissue quality and collagen architecture. Electromechanical QP also correlated strongly with biomechanical properties including fibril modulus (r = -0.76, P < 0.0001), matrix modulus (r = -0.69, P < 0.0001), and log of permeability (r = 0.72, P < 0.0001). The QP correlated weakly with GAG per wet weight and with water content (r = -0.50, P < 0.0003 and r = 0.39, P < 0.006 respectively). Non-destructive electromechanical QP measurements correlate strongly with histological scores and biomechanical parameters providing a rapid and reliable assessment of articular cartilage quality. Copyright © 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

Evaluation of quantification methods for real-time PCR minor groove binding hybridization probe assays.

PubMed

Durtschi, Jacob D; Stevenson, Jeffery; Hymas, Weston; Voelkerding, Karl V

2007-02-01

Real-time PCR data analysis for quantification has been the subject of many studies aimed at the identification of new and improved quantification methods. Several analysis methods have been proposed as superior alternatives to the common variations of the threshold crossing method. Notably, sigmoidal and exponential curve fit methods have been proposed. However, these studies have primarily analyzed real-time PCR with intercalating dyes such as SYBR Green. Clinical real-time PCR assays, in contrast, often employ fluorescent probes whose real-time amplification fluorescence curves differ from those of intercalating dyes. In the current study, we compared four analysis methods related to recent literature: two versions of the threshold crossing method, a second derivative maximum method, and a sigmoidal curve fit method. These methods were applied to a clinically relevant real-time human herpes virus type 6 (HHV6) PCR assay that used a minor groove binding (MGB) Eclipse hybridization probe as well as an Epstein-Barr virus (EBV) PCR assay that used an MGB Pleiades hybridization probe. We found that the crossing threshold method yielded more precise results when analyzing the HHV6 assay, which was characterized by lower signal/noise and less developed amplification curve plateaus. In contrast, the EBV assay, characterized by greater signal/noise and amplification curves with plateau regions similar to those observed with intercalating dyes, gave results with statistically similar precision by all four analysis methods.

Use of the Filovirus Animal Non-Clinical Group (FANG) Ebola virus immuno-assay requires fewer study participants to power a study than the Alpha Diagnostic International assay.

PubMed

Logue, James; Tuznik, Kaylie; Follmann, Dean; Grandits, Greg; Marchand, Jonathan; Reilly, Cavan; Sarro, Yeya Dit Sadio; Pettitt, James; Stavale, Eric J; Fallah, Mosoka; Olinger, Gene G; Bolay, Fatorma K; Hensley, Lisa E

2018-05-01

As part of the scientific community's development of medical countermeasures against Ebola virus disease, optimization of standardized assays for product evaluation is paramount. The recent outbreak heightened awareness to the scarcity of available assays and limited information on performance and reproducibility. To evaluate the immunogenicity of vaccines entering Phase I-III trials and to identify survivors, two enzyme-linked immunosorbent assays, the Filovirus Animal Non-Clinical Group assay and the Alpha Diagnostics International assay, were evaluated for detection of immunoglobulin G against Ebola virus glycoprotein. We found that the Filovirus Animal Nonclinical Group assay produced a wider range of relative antibody concentrations, higher assay precision, larger relative accuracy range, and lower regional background. Additionally, to sufficiently power a vaccine trial, use of the Filovirus Animal Nonclinical Group assay would require one third the number of participants than the Alpha Diagnostics International assay. This reduction in needed study participants will require less money, fewer man hours, and much less time to evaluate vaccine immunogenicity. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Novel enzymatic method for assaying Lp-PLA2 in serum.

PubMed

Yamaura, Saki; Sakasegawa, Shin-Ichi; Koguma, Emisa; Ueda, Shigeru; Kayamori, Yuzo; Sugimori, Daisuke; Karasawa, Ken

2018-06-01

Measurement of lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) can be used as an adjunct to traditional cardiovascular risk factors for identifying individuals at higher risk of cardiovascular events. This can be performed by quantification of the protein concentration using an ELISA platform or by measuring Lp-PLA 2 activity using platelet-activating factor (PAF) analog as substrate. Here, an enzymatic Lp-PLA 2 activity assay method using 1-O-Hexadecyl-2-acetyl-rac-glycero-3-phosphocholine (rac C 16 PAF) was developed. The newly revealed substrate specificity of lysoplasmalogen-specific phospholipase D (lysophospholipase D (LysoPLD)) was exploited. Lp-PLA 2 hydrolyzes 1-O-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (C 16 PAF) to 1-O-Hexadecyl-2-hydroxy-sn-glycero-3-phosphocholine (LysoPAF). LysoPLD acted on LysoPAF, and the hydrolytically released choline was detected by choline oxidase. Regression analysis of Lp-PLA 2 activity measured by the enzymatic Lp-PLA 2 activity assay vs. two chemical Lp-PLA 2 activity assays, i.e. LpPLA 2 FS and PLAC® test, and ELISA, gave the following correlation coefficients: 0.990, 0.893 and 0.785, respectively (n = 30). Advantages of this enzymatic Lp-PLA 2 activity assay compared with chemical Lp-PLA 2 methods include the following; (i) only requires two reagents enabling a simple two-point linear calibration method with one calibrator (ii) no need for inhibitors of esterase-like activity in serum. Copyright © 2018 Elsevier B.V. All rights reserved.

Destruction of chloroanisoles by using a hydrogen peroxide activated method and its application to remove chloroanisoles from cork stoppers.

PubMed

Recio, Eliseo; Alvarez-Rodríguez, María Luisa; Rumbero, Angel; Garzón, Enrique; Coque, Juan José R

2011-12-14

A chemical method for the efficient destruction of 2,4,6-trichloroanisole (TCA) and pentachloroanisole (PCA) in aqueous solutions by using hydrogen peroxide as an oxidant catalyzed by molybdate ions in alkaline conditions was developed. Under optimal conditions, more than 80.0% TCA and 75.8% PCA were degraded within the first 60 min of reaction. Chloroanisoles destruction was followed by a concomitant release of up to 2.9 chloride ions per TCA molecule and 4.6 chloride ions per PCA molecule, indicating an almost complete dehalogenation of chloroanisoles. This method was modified to be adapted to chloroanisoles removal from the surface of cork materials including natural cork stoppers (86.0% decrease in releasable TCA content), agglomerated corks (78.2%), and granulated cork (51.3%). This method has proved to be efficient and inexpensive with practical application in the cork industry to lower TCA levels in cork materials.

Measurement of the distribution of non-structural carbohydrate composition in onion populations by a high-throughput microplate enzymatic assay.

PubMed

Revanna, Roopashree; Turnbull, Matthew H; Shaw, Martin L; Wright, Kathryn M; Butler, Ruth C; Jameson, Paula E; McCallum, John A

2013-08-15

Non-structural carbohydrate (NSC; glucose, fructose, sucrose and fructan) composition of onions (Allium cepa L.) varies widely and is a key determinant of market usage. To analyse the physiology and genetics of onion carbohydrate metabolism and to enable selective breeding, an inexpensive, reliable and practicable sugar assay is required to phenotype large numbers of samples. A rapid, reliable and cost-effective microplate-based assay was developed for NSC analysis in onions and used to characterise variation in tissue hexose, sucrose and fructan content in open-pollinated breeding populations and in mapping populations developed from a wide onion cross. Sucrose measured in microplates employing maltase as a hydrolytic enzyme was in agreement with HPLC-PAD results. The method revealed significant variation in bulb fructan content within open-pollinated 'Pukekohe Longkeeper' breeding populations over a threefold range. Very wide segregation from 80 to 600 g kg(-1) in fructan content was observed in bulbs of F2 genetic mapping populations from the wide onion cross 'Nasik Red × CUDH2150'. The microplate enzymatic assay is a reliable and practicable method for onion sugar analysis for genetics, breeding and food technology. Open-pollinated onion populations may harbour extensive within-population variability in carbohydrate content, which may be quantified and exploited using this method. The phenotypic data obtained from genetic mapping populations show that the method is well suited to detailed genetic and physiological analysis. © 2013 Society of Chemical Industry.

Hypergolic Propellant Destruction Evaluation Cost Benefit Analysis

NASA Technical Reports Server (NTRS)

Kessel, Kurt

2010-01-01

At space vehicle launch sites such as Vandenberg Air Force Base (VAFB), Cape Canaveral Air Force Station (CCAFS) and Kennedy Space Center (KSC), toxic vapors and hazardous liquid wastes result from the handling of commodities (hypergolic fuels and oxidizers), most notably from transfer operations where fuel and oxidizer are transferred from bulk storage tanks or transfer tankers to space launch vehicles. During commodity transfer at CCAFS and KSC, wet chemical scrubbers (typically containing four scrubbing towers) are used to neutralize fuel saturated vapors from vent systems on tanks and tanker trailers. For fuel vapors, a citric acid solution is used to scrub out most of the hydrazine. Operation of both the hypergolic fuel and oxidizer vapor scrubbers generates waste scrubber liquor. Currently, scrubber liquor from the fuel vapor scrubber is considered non-hazardous. The scrubber liquor is defined as spent citric acid scrubber solution; the solution contains complexed hydrazine I methylhydrazine and is used to neutralize nonspecification hypergolic fuel generated by CCAFS and KSC. This project is a collaborative effort between Air Force Space Command (AFSPC), Space and Missile Center (SMC), the CCAFS, and National Aeronautics and Space Administration (NASA) to evaluate microwave destruction technology for the treatment of non-specification hypergolic fuel generated at CCAFS and KSC. The project will capitalize on knowledge gained from microwave treatment work being accomplished by AFSPC and SMC at V AFB. This report focuses on the costs associated with the current non-specification hypergolic fuel neutralization process (Section 2.0) as well as the estimated costs of operating a mobile microwave unit to treat non-specification hypergolic fuel (Section 3.0), and compares the costs for each (Section 4.0).The purpose of this document is to assess the costs associated with waste hypergolic fuel. This document will report the costs associated with the current fuel

A non-destructive internal nuclear forensic investigation at Argonne: discovery of a Pu planchet from 1948

DOE PAGES

Savina, Joseph A.; Steeb, Jennifer L.; Savina, Michael R.; ...

2016-06-02

A plutonium alpha standard dating from 1948 was discovered at Argonne National Laboratory and characterized using a number of non-destructive analytical techniques. The principle radioactive isotope was found to be 239Pu and unique ring structures were found across the surface of the deposition area. Due to chronological constraints on possible sources and its high isotopic purity, the plutonium in the sample was likely produced by the Oak Ridge National Lab X-10 Reactor. As a result, it is proposed that the rings are resultant through a combination of polishing and electrodeposition, though the hypothesis fails to address a few key featuresmore » of the ring structures.« less

Intracellular gold nanoparticles enhance non-invasive radiofrequency thermal destruction of human gastrointestinal cancer cells

PubMed Central

Gannon, Christopher J; Patra, Chitta Ranjan; Bhattacharya, Resham; Mukherjee, Priyabrata; Curley, Steven A

2008-01-01

Background Novel approaches to treat human cancer that are effective with minimal toxicity profiles are needed. We evaluated gold nanoparticles (GNPs) in human hepatocellular and pancreatic cancer cells to determine: 1) absence of intrinsic cytotoxicity of the GNPs and 2) external radiofrequency (RF) field-induced heating of intracellular GNPs to produce thermal destruction of malignant cells. GNPs (5 nm diameter) were added to 2 human cancer cell lines (Panc-1, Hep3B). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and propidium iodide-fluorescence associated cell sorting (PI-FACS) assessed cell proliferation and GNP-related cytotoxicity. Other GNP-treated cells were exposed to a 13.56 MHz RF field for 1, 2, or 5 minutes, and then incubated for 24 hours. PI-FACS measured RF-induced cytotoxicity. Results GNPs had no impact on cellular proliferation by MTT assay. PI-FACS confirmed that GNPs alone produced no cytotoxicity. A GNP dose-dependent RF-induced cytotoxicity was observed. For Hep3B cells treated with a 67 μM/L dose of GNPs, cytotoxicity at 1, 2 and 5 minutes of RF was 99.0%, 98.5%, and 99.8%. For Panc-1 cells treated at the 67 μM/L dose, cytotoxicity at 1, 2, and 5 minutes of RF was 98.5%, 98.7%, and 96.5%. Lower doses of GNPs were associated with significantly lower rates of RF-induced thermal cytotoxicity for each cell line (P < 0.01). Cells not treated with GNPs but treated with RF for identical time-points had less cytotoxicity (Hep3B: 17.6%, 21%, and 75%; Panc-1: 15.3%, 26.4%, and 39.8%, all P < 0.01). Conclusion We demonstrate that GNPs 1) have no intrinsic cytotoxicity or anti-proliferative effects in two human cancer cell lines in vitro and 2) GNPs release heat in a focused external RF field. This RF-induced heat release is lethal to cancer cells bearing intracellular GNPs in vitro. PMID:18234109

Evaluation of reinforced concrete structures using the electromagnetic method

NASA Astrophysics Data System (ADS)

Chady, Tomasz; Frankowski, Paweł; Waszczuk, Paweł; Zieliński, Adam

2018-04-01

Reinforced concrete has been a universally dominant construction material for over a century, although structures made of this material are often exposed to many types of damage and deterioration due to different causes and external conditions. The most important problem is corrosion of the reinforcement. Currently, most of the inspection methods of rebar in concrete are of an indirect nature or they are partially destructive. Moreover, none of the well-known systems allow for direct and non-destructive evaluation of the rebar corrosion. The purpose of this paper is to present the new, direct and non-destructive method, which allows detection of cracks and corrosion of the reinforcement bars.

Self-Destructive Behavior in People with Dissociative Disorders.

ERIC Educational Resources Information Center

Saxe, Glenn N.; Chawla, Neharika; Van der Kolk, Bessel

2002-01-01

Study assesses self-destructive behavior in a group of inpatients who have dissociative disorders compared to those who report few dissociative symptoms. Results reveal that these patients more frequently engage in self-destructive behaviors, use more methods of self-injury, and begin to injure themselves at an earlier age then patients who do not…

Non-destructive monitoring of creaming of oil-in-water emulsion-based formulations using magnetic resonance imaging.

PubMed

Onuki, Yoshinori; Horita, Akihiro; Kuribayashi, Hideto; Okuno, Yoshihide; Obata, Yasuko; Takayama, Kozo

2014-07-01

A non-destructive method for monitoring creaming of emulsion-based formulations is in great demand because it allows us to understand fully their instability mechanisms. This study was aimed at demonstrating the usefulness of magnetic resonance (MR) techniques, including MR imaging (MRI) and MR spectroscopy (MRS), for evaluating the physicochemical stability of emulsion-based formulations. Emulsions that are applicable as the base of practical skin creams were used as test samples. Substantial creaming was developed by centrifugation, which was then monitored by MRI. The creaming oil droplet layer and aqueous phase were clearly distinguished by quantitative MRI by measuring T1 and the apparent diffusion coefficient. Components in a selected volume in the emulsions could be analyzed using MRS. Then, model emulsions having different hydrophilic-lipophilic balance (HLB) values were tested, and the optimal HLB value for a stable dispersion was determined. In addition, the MRI examination enables the detection of creaming occurring in a polyethylene tube, which is commonly used for commercial products, without losing any image quality. These findings strongly indicate that MR techniques are powerful tools to evaluate the physicochemical stability of emulsion-based formulations. This study will make a great contribution to the development and quality control of emulsion-based formulations.

Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors

PubMed Central

Simko, Ivan; Hayes, Ryan J.; Furbank, Robert T.

2016-01-01

Rapid development of plants is important for the production of ‘baby-leaf’ lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-destructive evaluations of plants can provide valuable information to breeders and growers. The objective of the present study was to test the feasibility of using non-destructive phenotyping with optical sensors for the evaluations of lettuce plants in early stages of development. We performed the series of experiments to determine if hyperspectral imaging and chlorophyll fluorescence imaging can determine phenotypic changes manifested on lettuce plants subjected to the extreme temperature and salinity stress treatments. Our results indicate that top view optical sensors alone can accurately determine plant size to approximately 7 g fresh weight. Hyperspectral imaging analysis was able to detect changes in the total chlorophyll (RCC) and anthocyanin (RAC) content, while chlorophyll fluorescence imaging revealed photoinhibition and reduction of plant growth caused by the extreme growing temperatures (3 and 39°C) and salinity (100 mM NaCl). Though no significant correlation was found between Fv/Fm and decrease in plant growth due to stress when comparisons were made across multiple accessions, our results indicate that lettuce plants have a high adaptability to both low (3°C) and high (39°C) temperatures, with no permanent damage to photosynthetic apparatus and fast recovery of plants after moving them to the optimal (21°C) temperature. We have also detected a strong relationship between visual rating of the green- and red-leaf color intensity and RCC and RAC, respectively. Differences in RAC among accessions suggest that the selection for intense red color may be easier to perform at somewhat lower than the optimal temperature. This study serves as a proof of

Rapid, non-destructive coral paleothermometry by synchrotron XR

NASA Astrophysics Data System (ADS)

Tangri, N.; Mehta, A.; Marks, R.; Dunbar, R. B.

2016-12-01

We present advances in the use of synchrotron x-ray fluorescence (XRF) to recover climate signals from coral exoskeleton. Corals record sea surface temperature (SST), salinity, and other environmental conditions in the density and composition of their exoskeletons; in particular, SST is reflected in both the Sr/Ca ratio and the annual density banding. Synchrotron XRF has previously been used to examine the fine-scaled variability of Sr concentrations in the exoskeleton structure, but has not yet yielded any long-term SST reconstructions. Modern XRF techniques allow the detection of sub-ppm trace element concentrations and appear ideally suited to long climate reconstructions, as they are non-destructive, high-resolution (250 um) and potentially quite rapid ( 40 years of sample in 24 hours of instrument time). The low Sr content of the coral and its low change in concentration require a high brightness synchrotron source to generate a high signal-to-background ratio. However, difficulties arise from the local heterogeneity of Sr that is unrelated to environmental conditions. These variations of biological origin in Sr concentrations often mask the smaller-amplitude, annual and interannual SST signals. The challenge is to normalize the local variability in order to extract the climate signal. Other techniques have normalized against Ca, but in XRF the Ca signal is sensitive to only the surface 50 um of material, whereas the Sr signal comes from 1mm, so the values are not comparable. Instead, we normalize against density as calculated from beam transmission. We also explore the use of Rb normalization to filter out collection artifacts. Both Sr and Rb show strong annual signals and interesting departures from the density signal. Finally, we pair the XRF results with δ18O measurements to recover a convincing record of SST variation. Although challenges remain, we believe that synchrotron XRF techniques hold considerable promise to rapidly and accurately recover

A technical study of alloy compositions of "brass" wind musical instruments (1651-1867) utilizing non-destructive X-ray fluorescence

NASA Astrophysics Data System (ADS)

Bacon, Alice Louise

This thesis represents a new interdisciplinary approach to the conservation, care and curatorial study of 'brass' wind musical instruments. It attempts to combine metallurgical, chronological and historical aspects for a selection of instruments. The research consists of the systematic study of seventy-seven musical instruments, by known makers, using standardised non-destructive energy dispersive x-ray fluorescence (XRF). Such compositional data are virtually non-existent for historical 'brass' instruments in Britain and what few technical data that do exist are sporadic in quantity and quality. The development of brass instruments is interwoven with the history of brass making, but because there are a limited number of appropriate examples such links can be difficult to identify. This thesis describes the development of brass production from the cementation process to the commercial production of zinc and modern brass. Its relationship to the musical instrument industry in Britain is linked with historical evidence. It will be shown that innovation and known historical metallurgical achievements are reflected in the compositional changes of the alloys used for musical instruments. This thesis focuses on specific named brass wind musical instrument makers. This thesis sets out to investigate the extent to which a single analytical technique such as non-destructive analysis utilising XRF could be useful in the curatorial and conservation care of musical instruments. The results of the analyses revealed new aspects to the use of metals for making musical instruments. They give new information on approximate alloy compositions and, in particular, the results have shown that in the seventeenth-century in England, a ternary alloy of copper/tin/zinc was used, and that it was, perhaps, only superseded by brass (copper/zinc alloy) in the eighteenth century. It has been possible to arrange the results into a chronology of alloys particularly reflecting the change from the

Development of an instrument for non-destructive identification of Unexploded Ordnance using tagged neutrons - a proof of concept study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitra, S.; Dioszegi, I.

2011-10-23

Range clearance operations at munitions testing grounds must discriminate Unexploded Ordnance (UXO) from clutter items and distinguish UXO filled with High Explosives (HE) from those with inert fillers. Non-destructive technologies are thus necessary for the cost-effective disposal of UXO during remediation of such sites. The only technique showing promise so far for the non-destructive elemental characterization of UXO fillers utilizes neutron interactions with the material to detect carbon (C), nitrogen (N) and oxygen (O) which have unique ratios in HE. However, several unresolved issues hinder the wide application of this potentially very suitable technique. The most important one is thatmore » neutrons interact with all surrounding matter in addition to the interrogated material, leading to a very high gamma-ray background in the detector. Systems requiring bulky shielding and having poor signal-to-noise ratios (SNRs) for measuring elements are unsuitable for field deployment. The inadequacies of conventional neutron interrogation methods are overcome by using the tagged-neutron approach, and the availability of compact sealed neutron generators exploiting this technique offers field deployment of non-intrusive measurement systems for detecting threat materials, like explosives and drugs. By accelerating deuterium ions into a tritium target, the subsequent fusion reaction generates nearly back-to-back emissions of neutrons and alpha particles of energy 14.1 and 3.5 MeV respectively. A position-sensitive detector recognizes the associated alpha particle, thus furnishing the direction of the neutron. The tagged neutrons interact with the nuclei of the interrogated object, producing element-specific prompt gamma-rays that the gamma detectors recognize. Measuring the delay between the detections of the alpha particle and the gamma-ray determines where the reaction occurred along the axis of the neutron beam (14.1 MeV neutrons travel at 5 cm/nanosecond, while

Non destructive technique for cracks detection by an eddy current in differential mode for steel frames

NASA Astrophysics Data System (ADS)

Harzalla, S.; Belgacem, F. Bin Muhammad; Chabaat, M.

2014-12-01

In this paper, a nondestructive technique is used as a tool to control cracks and microcracks in materials. A simulation by a numerical approach such as the finite element method is employed to detect cracks and eventually; to study their propagation using a crucial parameter such as the stress intensity factor. This approach has been used in the aircraft industry to control cracks. Besides, it makes it possible to highlight the defects of parts while preserving the integrity of the controlled products. On the other side, it is proven that the reliability of the control of defects gives convincing results for the improvement of the quality and the safety of the material. Eddy current testing (ECT) is a standard technique in industry for the detection of surface breaking flaws in magnetic materials such as steels. In this context, simulation tools can be used to improve the understanding of experimental signals, optimize the design of sensors or evaluate the performance of ECT procedures. CEA-LIST has developed for many years semi-analytical models embedded into the simulation platform CIVA dedicated to non-destructive testing. The developments presented herein address the case of flaws located inside a planar and magnetic medium. Simulation results are obtained through the application of the Volume Integral Method (VIM). When considering the ECT of a single flaw, a system of two differential equations is derived from Maxwell equations. The numerical resolution of the system is carried out using the classical Galerkin variant of the Method of Moments. Besides, a probe response is calculated by application of the Lorentz reciprocity theorem. Finally, the approach itself as well as comparisons between simulation results and measured data are presented.

How do we know if plants in our nursery have Phytophthora? Detection methods and an integrated approach to monitoring

Treesearch

Christa Conforti

2017-01-01

A Phytophthora cactorum-infected nursery crop of Ceanothus thyrsiflorus was used to evaluate three Phytophthora monitoring methods. The Phytophthora detection level of three non-destructive sampling methods was quantified and compared to the detection level of destructive sampling. Non-...

Self-Destructing Dark Matter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grossman, Yuval; Harnik, Roni; Telem, Ofri

We present Self-Destructing Dark Matter (SDDM), a new class of dark matter models which are detectable in large neutrino detectors. In this class of models, a component of dark matter can transition from a long-lived state to a short-lived one by scattering off of a nucleus or an electron in the Earth. The short-lived state then decays to Standard Model particles, generating a dark matter signal with a visible energy of order the dark matter mass rather than just its recoil. This leads to striking signals in large detectors with high energy thresholds. We present a few examples of modelsmore » which exhibit self destruction, all inspired by bound state dynamics in the Standard Model. The models under consideration exhibit a rich phenomenology, possibly featuring events with one, two, or even three lepton pairs, each with a fixed invariant mass and a fixed energy, as well as non-trivial directional distributions. This motivates dedicated searches for dark matter in large underground detectors such as Super-K, Borexino, SNO+, and DUNE.« less

Non-inflammatory destructive periodontal disease: a clinical, microbiological, immunological and genetic investigation

PubMed Central

REPEKE, Carlos Eduardo; CARDOSO, Cristina Ribeiro; CLAUDINO, Marcela; SILVEIRA, Elcia Maria; TROMBONE, Ana Paula Favaro; CAMPANELLI, Ana Paula; SILVA, João Santana; MARTINS JÚNIOR, Walter; GARLET, Gustavo Pompermaier

2012-01-01

Periodontitis comprises a group of multifactorial diseases in which periodontopathogens accumulate in dental plaque and trigger host chronic inflammatory and immune responses against periodontal structures, which are determinant to the disease outcome. Although unusual cases of non-inflammatory destructive periodontal disease (NIDPD) are described, their pathogenesis remains unknown. A unique NIDPD case was investigated by clinical, microbiological, immunological and genetic tools. The patient, a non-smoking dental surgeon with excessive oral hygiene practice, presented a generalized bone resorption and tooth mobility, but not gingival inflammation or occlusion problems. No hematological, immunological or endocrine alterations were found. No periodontopathogens (A. actinomycetemcomitans, P. gingivalis, F. nucleatum and T. denticola) or viruses (HCMV, EBV-1 and HSV-1) were detected, along with levels of IL-1β and TNF-α in GCF compatible with healthy tissues. Conversely ALP, ACP and RANKL GCF levels were similar to diseased periodontal sites. Genetic investigation demonstrated that the patient carried some SNPs, as well HLA-DR4 (*0404) and HLA-B27 alleles, considered risk factors for bone loss. Then, a less vigorous and diminished frequency of toothbrushing was recommended to the patient, resulting in the arrest of alveolar bone loss, associated with the return of ALP, ACP and RANKL in GCF to normality levels. In conclusion, the unusual case presented here is compatible with the previous description of NIDPD, and the results that a possible combination of excessive force and frequency of mechanical stimulation with a potentially bone loss prone genotype could result in the alveolar bone loss seen in NIDPD. PMID:22437688

Non-destructive assessment of grapevine water status in the field using a portable NIR spectrophotometer.

PubMed

Tardaguila, Javier; Fernández-Novales, Juan; Gutiérrez, Salvador; Diago, Maria Paz

2017-08-01

Until now, the majority of methods employed to assess grapevine water status have been destructive, time-intensive, costly and provide information of a limited number of samples, thus the ability of revealing within-field water status variability is reduced. The goal of this work was to evaluate the capability of non-invasive, portable near infrared (NIR) spectroscopy acquired in the field, to assess the grapevine water status in diverse varieties, grown under different environmental conditions, in a fast and reliable way. The research was conducted 2 weeks before harvest in 2012, in two commercial vineyards, planted with eight different varieties. Spectral measurements were acquired in the field on the adaxial and abaxial sides of 160 individual leaves (20 leaves per variety) using a commercially available handheld spectrophotometer (1600-2400 nm). Principal component analysis (PCA) and modified partial least squares (MPLS) were used to interpret the spectra and to develop reliable prediction models for stem water potential (Ψ s ) (cross-validation correlation coefficient (r cv ) ranged from 0.77 to 0.93, and standard error of cross validation (SECV) ranged from 0.10 to 0.23), and leaf relative water content (RWC) (r cv ranged from 0.66 to 0.81, and SECV between 1.93 and 3.20). The performance differences between models built from abaxial and adaxial-acquired spectra is also discussed. The capability of non-invasive NIR spectroscopy to reliably assess the grapevine water status under field conditions was proved. This technique can be a suitable and promising tool to appraise within-field variability of plant water status, helpful to define optimised irrigation strategies in the wine industry. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

[Diagnostic and curative bronchoscopy for purulent-destructive pulmonary diseases].

PubMed

Pinchuk, T P; Yasnogorodsky, O O; Guryanova, Yu V; Taldykin, M V; Kachikin, A S; Catane, Yu A

To assess an efficacy of diagnostic and curative bronchoscopy in patients with purulent-destructive pulmonary diseases. Diagnosis and treatment of 34 patients with purulent-destructive pulmonary diseases including small-focal destruction (14) and lung abscesses (19) were analyzed. 33 patients underwent diagnostic fibrobronchoscopy (FBS) with brush and transbronchial biopsy. Curative endoscopy included bronchial tree sanation, peribronchial administration of antibiotics (5) and transbronchial drainage of abscess (14). Atrophic bronchitis and cicatricial deformity of the 2-3rd segmental bronchi were revealed in 81.8% and 15.2% respectively. Transbronchial biopsy confirmed malignant neoplasms (15.2%) and pulmonary tuberculosis (6.1%). Peribronchial administration of amikacin in patients with small-focal pulmonary destruction and transbronchial drainage of abscesses accelerated pulmonary tissue repair and complete recovery. Transbronchial biopsy in patients with destructive pulmonary diseases verifies pathological process and excludes malignant and specific pulmonary damage. Complex use of endoscopic methods is associated with positive clinical result in all patients with pulmonary destruction.

Shearography for Non-destructive Inspection with applications to BAT Mask Tile Adhesive Bonding and Specular Surface Honeycomb Panels

NASA Technical Reports Server (NTRS)

Lysak, Daniel B.

2003-01-01

The applicability of shearography techniques for non-destructive evaluation in two unique application areas is examined. In the first application, shearography is used to evaluate the quality of adhesive bonds holding lead tiles to the B.4T gamma ray mask for the NASA Swift program. Using a vibration excitation, the more poorly bonded tiles are readily identifiable in the shearography image. A quantitative analysis is presented that compares the shearography results with a destructive pull test measuring the force at bond failure. The second application is to evaluate the bonding between the skin and core of a honeycomb structure with a specular (mirror-like) surface. In standard shearography techniques, the object under test must have a diffuse surface to generate the speckle patterns in laser light, which are then sheared. A novel configuration using the specular surface as a mirror to image speckles from a diffuser is presented, opening up the use of shearography to a new class of objects that could not have been examined with the traditional approach. This new technique readily identifies large scale bond failures in the panel, demonstrating the validity of this approach.

A cylinder-plate method for microbiological assay of clavulanic acid.

PubMed

Hamedi, J; Shahverdi, A R; Samadi, N; Mohammadi, A; Shiran, M; Akhondi, S

2006-12-01

Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory effect of clavulanic acid in combination with penicillin G upon Escherichia coli ATCC 35218, which is used as the test organism. The correlation between clavulanic acid concentration and the inhibitory effect on E. coli was linear (r > 0.99) and in the range of 8-20 microg/ml. These results indicate that the proposed method is appropriate for the determination of clavulanic acid in commercial samples and can be used in routine quality control.

Bioanalytical method transfer considerations of chromatographic-based assays.

PubMed

Williard, Clark V

2016-07-01

Bioanalysis is an important part of the modern drug development process. The business practice of outsourcing and transferring bioanalytical methods from laboratory to laboratory has increasingly become a crucial strategy for successful and efficient delivery of therapies to the market. This chapter discusses important considerations when transferring various types of chromatographic-based assays in today's pharmaceutical research and development environment.

Evaluation of a new ultrasensitive assay for cardiac troponin I.

PubMed

Casals, Gregori; Filella, Xavier; Bedini, Josep Lluis

2007-12-01

We evaluated the analytical and clinical performance of a new ultrasensitive cardiac troponin I assay (cTnI) on the ADVIA Centaur system (TnI-Ultra). The evaluation included the determination of detection limit, within-assay and between-assay variation and comparison with two other non-ultrasensitive methods. Moreover, cTnI was determined in 120 patients with acute chest pain with three methods. To evaluate the ability of the new method to detect MI earlier, it was assayed in 8 MI patients who first tested negative then positive by the other methods. The detection limit was 0.009 microg/L and imprecision was <10% at all concentrations evaluated. In comparison with two other methods, 10% of the anginas diagnosed were recategorized to MI. The ADVIA Centaur TnI-Ultra assay presented high reproducibility and high sensitivity. The use of the recommended lower cutpoint (0.044 microg/L) implied an increased and earlier identification of MI.

Melatonin prevents hypochlorous acid-mediated cyanocobalamin destruction and cyanogen chloride generation.

PubMed

Jeelani, Roohi; Maitra, Dhiman; Chatzicharalampous, Charalampos; Najeemuddin, Syed; Morris, Robert T; Abu-Soud, Husam M

2018-04-01

Hypochlorous acid (HOCl) is a potent cytotoxic oxidant generated by the enzyme myeloperoxidase (MPO) in the presence of hydrogen peroxide (H 2 O 2 ) and chloride (Cl - ). Elevated levels of HOCl play an important role in various pathological conditions through oxidative modification of several biomolecules. Recently, we have highlighted the ability of HOCl to mediate the destruction of the metal-ion derivatives of tetrapyrrole macrocyclic rings such as hemoproteins and vitamin B 12 (VB 12 ) derivatives. Destruction of cyanocobalamin, a common pharmacological form of VB 12 mediated by HOCl, results in the generation of toxic molecular products such as chlorinated derivatives, corrin ring cleavage products, the toxic blood agents cyanide (CN - ) and cyanogen chloride (CNCl), and redox-active free cobalt. Here, we show that melatonin prevents HOCl-mediated cyanocobalamin destruction, using a combination of UV-Vis spectrophotometry, high-performance liquid chromatography analysis, and colorimetric CNCl assay. Identification of several melatonin oxidation products suggests that the protective role of melatonin against HOCl-mediated cyanocobalamin destruction and subsequent CNCl generation is at the expense of melatonin oxidation. Collectively, this work highlights that, in addition to acting as an antioxidant and as a MPO inhibitor, melatonin can also prevent VB 12 deficiency in inflammatory conditions such as cardiovascular and neurodegenerative diseases, among many others. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Ultrasonic destruction of albumin microbubbles enhances gene transfection and expression in cardiac myocytes.

PubMed

Wang, Guo-zhong; Liu, Jing-hua; Lü, Shu-zheng; Lü, Yun; Guo, Cheng-jun; Zhao, Dong-hui; Fang, Dong-ping; He, Dong-fang; Zhou, Yuan; Ge, Chang-jiang

2011-05-01

destruction of microbubbles might be a promising method for the delivery of non-viral DNA into cardiac myocytes, and the gene tranfection is related to the characteristics of microbubbles.

Uranium daughter growth must not be neglected when adjusting plutonium materials for assay and isotopic contents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marsh, S.F.; Spall, W.D.; Abernathey, R.M.

1976-11-01

Relationships are provided to compute the decreasing plutonium content and changing isotopic distribution of plutonium materials for the radioactive decay of /sup 238/Pu, /sup 239/Pu, /sup 240/Pu and /sup 242/Pu to long-lived uranium daughters and of /sup 241/Pu to /sup 241/Am. This computation is important to the use of plutonium reference materials to calibrate destructive and nondestructive methods for assay and isotopic measurements, as well as to accountability inventory calculations.