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Sample records for nova-like bb doradus

  1. Spectroscopy from the Hubble Space Telescope Cosmic Origins Spectrograph of the Southern Nova-like BB Doradus in an Intermediate State

    NASA Astrophysics Data System (ADS)

    Godon, Patrick; Sion, Edward M.; Gänsicke, Boris T.; Hubeny, Ivan; de Martino, Domitilla; Pala, Anna F.; Rodríguez-Gil, Pablo; Szkody, Paula; Toloza, Odette

    2016-12-01

    We present a spectral analysis of the spectrum from the Hubble Space Telescope Cosmic Origins Spectrograph (HST/COS) of the southern VY Scl nova-like variable BB Doradus, obtained as part of a Cycle 20 HST/COS survey of accreting white dwarfs (WDs) in cataclysmic variables. BB Dor was observed with COS during an intermediate state with a low mass accretion rate, thereby allowing an estimate of the WD temperature. The results of our spectral analysis show that the WD is a significant far-ultraviolet (FUV) component of the spectrum with a temperature of about 35,000-50,000 K, assuming a WD mass of 0.80 {M}⊙ ({log}(g)=8.4). The disk, with a mass accretion rate of ≈ {10}-10 {M}⊙ {{yr}}-1, contributes about 1/5 to 1/2 of the FUV flux.

  2. The fight for accretion: discovery of intermittent mass transfer in BB Doradus in the low state

    NASA Astrophysics Data System (ADS)

    Rodríguez-Gil, P.; Schmidtobreick, L.; Long, K. S.; Gänsicke, B. T.; Torres, M. A. P.; Rubio-Díez, M. M.; Santander-García, M.

    2012-05-01

    Our long-term photometric monitoring of southern nova-like cataclysmic variables with the 1.3-m Small and Moderate Aperture Research Telescope System (SMARTS) telescope found BB Doradus fading from V˜ 14.3 towards a deep low state at V˜ 19.3 in 2008 April. Here we present time-resolved optical spectroscopy of BB Dor in this faint state in 2009. The optical spectrum in quiescence is a composite of a hot white dwarf with Teff= 30 000 ± 5000 K and a M3-M4 secondary star with narrow emission lines (mainly of the Balmer series and He I) superposed. We associate these narrow profiles with an origin on the donor star. An analysis of the radial velocity curve of the Hα emission from the donor star allowed the measurement of an orbital period of 0.154 095 ± 0.000 003 d (3.698 28 ± 0.000 07 h), different from all previous estimates. We detected episodic accretion events which veiled the spectra of both stars and radically changed the line profiles within a time-scale of tens of minutes. This shows that accretion is not completely quenched in the low state. During these accretion episodes the line wings are stronger and their radial velocity curve is delayed by ˜0.2 cycle, similar to that observed in SW Sex and AM Her stars in the high state, with respect to the motion of the white dwarf. Two scenarios are proposed to explain the extra emission: impact of the material on the outer edge of a cold, remnant accretion disc, or the combined action of a moderately magnetic white dwarf (B1≲ 5 MG) and the magnetic activity of the donor star.

  3. Nova-like variables

    NASA Technical Reports Server (NTRS)

    Ladous, Constanze

    1993-01-01

    On grounds of different observable characteristics five classes of nova-like objects are distinguished: the UX Ursae Majoris stars, the antidwarf novae, the DQ Herculis stars, the AM Herculis stars, and the AM Canum Venaticorum stars. Some objects have not been classified specifically. Nova-like stars share most observable features with dwarf novae, except for the outburst behavior. The understanding is that dwarf novae, UX Ursae Majoris stars, and anti-dwarf novae are basically the same sort of objects. The difference between them is that in UX Ursae Majoris stars the mass transfer through the accretion disc always is high so the disc is stationary all the time; in anti-dwarf novae for some reason the mass transfer occasionally drops considerably for some time, and in dwarf novae it is low enough for the disc to undergo semiperiodic changes between high and low accretion events. DQ Herculis stars are believed to possess weakly magnetic white dwarfs which disrupt the inner disc at some distance from the central star; the rotation of the white dwarf can be seen as an additional photometric period. In AM Herculis stars, a strongly magnetic white dwarf entirely prevents the formation of an accretion disk and at the same time locks the rotation of the white dwarf to the binary orbit. Finally, AM Canum Venaticorum stars are believed to be cataclysmic variables that consist of two white dwarf components.

  4. A Comprehensive Review of Nova-Like Variable Stars

    NASA Astrophysics Data System (ADS)

    Sion, Edward

    2016-07-01

    A comprehensive review of nova-like variable stars of the VY Sculptoris and UX Ursa Majoris subtypes is presented. All that has been learned, up to the present time, from observations in the X-ray, far ultraviolet, optical and infrared bandpasses will be discussed. Spectroscopic analyses of high optical brightness states and low optical brightness states of nova-like variables will be summarized. The application of standard and non-standard accretion disk models as well as boundary layer structure will be discussed. The results of searches for nova shells, the evolutionary state of nova-likes as well as new spectroscopic results on BZ Cam (the bow shock CV) and BB Dor will also be included. This work is supported by NASA ADP grants NNX13AF12G and NNX13AF11G to Villanova University.

  5. The Nova-like star RW Sextantis

    NASA Astrophysics Data System (ADS)

    Stokes, S. J.; Evans, J. M.; Bianchini, A.; Canterna, R.

    2000-12-01

    We have analyzed 17 medium resolution spectra of RW Sex taken in 1988 at La Silla in the spectral range is 4000-5000 Å with a dispersion of 60 Å/mm and spectral resolution of about 2 Å/pixel. The mean spectrum of the object shows the continuum energy distribution slightly brighter and steeper than that observed by Beuermann, Stasiewski and Schwope (1992). In both cases the slope seems to be steeper that the λ -2.33 power law predicted for standard accretion discs (see Warner 1995). This might be due to uncertain flux calibration or to the dramatic intrinsic variability of this nova-like system (Honeycutt et al. 1998). Like in Beuerman et al.'s, the hydrogen and the HeI lines appear in absorption with superimposed central emission components. Relatively weak emissions from HeII at λ λ 4542,4686 and the blend CIII+NIII at λ4640 -50 are also seen. The peaks of the narrow emissions components of Hβ , HeIλ4471 and HeIλ4922 have been measured using Gaussian fittings. The new ephemeris are: T0(HJD) = 2446486.5061 +/- 0.0010 + 0.245064 +/- 0.000004 The radial velocity curve produced by the absorption components of the hydrogen and the HeI lines are in antiphase with respect to that produced by the emission cores. The amplitudes of all the radial velocity curves are consistent with those shown by Beuermann, Stasiewski and Schwope (1992). According to these authors the absorption lines are produced in the optically thick accretion disc while the narrow emissions arise from the heated atmosphere of the secondary. We fail however to detect the broad emission components observed by these authors and attributed to the hot disc corona. This point should deserve future investigation.

  6. Nova-like cataclysmic variables in the infrared

    SciTech Connect

    Hoard, D. W.; Long, Knox S.; Howell, Steve B.; Wachter, Stefanie; Brinkworth, Carolyn S.; Knigge, Christian; Drew, J. E.; Szkody, Paula; Kafka, S.; Belle, Kunegunda; Ciardi, David R.; Froning, Cynthia S.; Van Belle, Gerard T.; Pretorius, M. L.

    2014-05-01

    Nova-like (NL) cataclysmic variables have persistently high mass transfer rates and prominent steady state accretion disks. We present an analysis of infrared observations of 12 NLs obtained from the Two Micron All Sky Survey, the Spitzer Space Telescope, and the Wide-field Infrared Survey Explorer All Sky Survey. The presence of an infrared excess at λ ≳ 3-5 μm over the expectation of a theoretical steady state accretion disk is ubiquitous in our sample. The strength of the infrared excess is not correlated with orbital period, but shows a statistically significant correlation (but shallow trend) with system inclination that might be partially (but not completely) linked to the increasing view of the cooler outer accretion disk and disk rim at higher inclinations. We discuss the possible origin of the infrared excess in terms of emission from bremsstrahlung or circumbinary dust, with either mechanism facilitated by the mass outflows (e.g., disk wind/corona, accretion stream overflow, and so on) present in NLs. Our comparison of the relative advantages and disadvantages of either mechanism for explaining the observations suggests that the situation is rather ambiguous, largely circumstantial, and in need of stricter observational constraints.

  7. NSV 1907 - A new eclipsing, nova-like cataclysmic variable

    NASA Astrophysics Data System (ADS)

    Hümmerich, Stefan; Gröbel, Rainer; Hambsch, Franz-Josef; Dubois, Franky; Ashley, Richard; Gänsicke, Boris T.; Vanaverbeke, Siegfried; Bernhard, Klaus; Wils, Patrick

    2017-01-01

    NSV 1907, formerly listed as an irregular variable in variability catalogues, was classified as an Algol-type eclipsing binary in the Catalina Surveys Periodic Variable Star Catalogue. We have identified NSV 1907 as an ultraviolet (UV) bright source using measurements from the GALEX space telescope and detected obvious out-of-eclipse variability in archival photometric data from the Catalina Sky Survey, which instigated a closer examination of the object. A spectrum and extensive multicolour photometric observations were acquired, from which we deduce that NSV 1907 is a deeply eclipsing, nova-like cataclysmic variable. Apart from the orbital variations (deep eclipses with a period of P ≈ 6.63 hours), changes in mean brightness and irregular short-term variability (flickering) were observed. The presence of a secondary minimum at phase φ ≈ 0.5 was established, which indicates a significant contribution of the companion star to the optical flux of the system. We find possible evidence for sinusoidal variations with a period of P ≈ 4.2 d, which we interpret as the nodal precession period of the accretion disc. No outbursts or VY Scl-like drops in brightness were detected either by the CSS or during our photometric monitoring. Because of its spectral characteristics and the observed variability pattern, we propose NSV 1907 as a new moderately bright long-period SW Sextantis star. Further photometric and spectroscopic observations are encouraged.

  8. Nova-like Cataclysmic Variables in the Infrared

    NASA Astrophysics Data System (ADS)

    Hoard, D. W.; Long, Knox S.; Howell, Steve B.; Wachter, Stefanie; Brinkworth, Carolyn S.; Knigge, Christian; Drew, J. E.; Szkody, Paula; Kafka, S.; Belle, Kunegunda; Ciardi, David R.; Froning, Cynthia S.; van Belle, Gerard T.; Pretorius, M. L.

    2014-05-01

    Nova-like (NL) cataclysmic variables have persistently high mass transfer rates and prominent steady state accretion disks. We present an analysis of infrared observations of 12 NLs obtained from the Two Micron All Sky Survey, the Spitzer Space Telescope, and the Wide-field Infrared Survey Explorer All Sky Survey. The presence of an infrared excess at λ >~ 3-5 μm over the expectation of a theoretical steady state accretion disk is ubiquitous in our sample. The strength of the infrared excess is not correlated with orbital period, but shows a statistically significant correlation (but shallow trend) with system inclination that might be partially (but not completely) linked to the increasing view of the cooler outer accretion disk and disk rim at higher inclinations. We discuss the possible origin of the infrared excess in terms of emission from bremsstrahlung or circumbinary dust, with either mechanism facilitated by the mass outflows (e.g., disk wind/corona, accretion stream overflow, and so on) present in NLs. Our comparison of the relative advantages and disadvantages of either mechanism for explaining the observations suggests that the situation is rather ambiguous, largely circumstantial, and in need of stricter observational constraints.

  9. Ultraviolet studies of nova-like variables with the IUE

    NASA Technical Reports Server (NTRS)

    Guinan, E. F.

    1983-01-01

    KQ Mon is a new UX UMa-type nova-like variable. Optical spectra taken in 1978 reveal very shallow Balmer absorption lines and He I (wavelength 4471) absorption. There was no evidence of orbital variations but the appearance of the optical spectrum and the presence of low amplitude flickering suggested a strong similarity to CD-42 degrees 14462 (=V3885 Sgr) and other members of the UX UMa class. KQ Mon was observed at low dispersion with the IUE satellite. Six spectra taken with the short wavelength prime (SWP) camera are dominated by strong broad absorption lines due to N V, O I, Si III, Si IV, C IV, He II, N IV, and A1 III. There is little evidence of orbital phase modulation over the time baseline of the observations. Unlike UV observations of other UX UMa-type objects, KQ Mon exhibits no emission lines or P Cygni-type profiles and the velocity displacements appear to be smaller, suggesting the absence of a hot, high velocity wind characterizing other UX UMa stars. The relationship of KQ Mon to other UX UMa disk stars is discussed and a model is suggested to explain their observed properties and the lack of major outbursts.

  10. Spectroscopy of γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Brunsden, E.; Pollard, K. R.; Cottrell, P. L.; Wright, D. J.; De Cat, P.; Kilmartin, P. M.

    2014-02-01

    The musician programme at the University of Canterbury has been successfully identifying pulsation modes in many γ Doradus stars using hundreds of ground-based spectroscopic observations. This paper describes some of the successful mode identifications and emerging patterns of the programme. The hybrid γ Doradus/δ Scuti star HD 49434 remains an enigma, despite the analysis of more than 1700 multi-site high-resolution spectra. A new result for this star is apparently distinct line-profile variations for the γ Doradus and δ Scuti frequencies.

  11. Models for various aspects of dwarf novae and nova-like stars

    NASA Technical Reports Server (NTRS)

    Ladous, Constanze

    1993-01-01

    The first attempts to explain the nature of dwarf novae were based on the assumption of single-star phenomena, in which emission lines were assumed to be caused by circumstellar gas shells. The outburst behavior was tentatively ascribed to the kind of (also not understood) mechanism leading to nova outbursts. The realization that some, and possibly all, dwarf novae and nova-like stars (and novae) are binaries eventually led to models which bore more and more similarities to the modern interpretation on the basis of the Roche model. Not all cataclysmic variables are known binaries. In fact, with respect to the entire number of known objects, the proven binaries are still the minority, but all the brightest variables are in fact known to binaries. Not a single system is known which exhibits the usual characteristics of a cataclysmic variable and at the same time can be declared with certainty to be a single star. Two systems are known, the dwarf nova EY Cyg and the recurrent nova V1017 Sgr, in which, in spite of intensive search, no radial velocity variations have been found; but they still exhibit composite spectra consisting of a bright continuum, an emission spectrum, and a cool absorption spectrum. If the Roche model is correct, it is to be expected that a small percentage of objects is viewed pole-on, so orbital motions do not make themselves felt as Doppler shifts of spectral lines. So even these two systems support the hypothesis that all cataclysmic variables (with the possible exception of symbiotic stars) are binaries. In cataclysmic variables, it seems that the brightness changes observed in dwarf novae and nova-like stars in the optical and the UV are due directly to changes in the accretion disks. The study and understanding of accretion disks in these systems can bear potentially valuable consequences for many other fields in astronomy. The observed spectra of dwarf novae and nova-like stars comprise a fairly large range: pure emission spectra, pure

  12. Radio Emission from Binary Stars in the AB Doradus Moving Group

    NASA Astrophysics Data System (ADS)

    Azulay, R.; Guirado, J. C.; Marcaide, J. M.; Martí-Vidal, I.; Ros, E.

    2016-01-01

    Precise determination of dynamical masses of pre-main-sequence stars is essential for calibrating stellar evolution models, that are widely used to derive theoretical masses of young low-mass objects. We have determined the individual masses of the pair AB Dor Ba/Bb using Australian Long Baseline Array observations and archive infrared data, as part of a larger program directed to monitor binary systems in the AB Doradus moving group. We have detected, for the first time, compact radio emission from both stars. This has allowed us to determine the orbital parameters of both the relative and absolute orbits and, consequently, their individual dynamical masses: 0.28+/-0.05 M⊙ and 0.25+/-0.05 M⊙. Comparisons of the dynamical masses with the prediction of pre-main-sequence (PMS) evolutionary models show that the models underpredict the dynamical masses of the binary components Ba and Bb by 10-30% and 10-40%, respectively.

  13. Far Ultraviolet Spectroscopy of Three Long Period Nova-Like Variables, V363 Aur, AC Cnc and RZ Gru

    NASA Astrophysics Data System (ADS)

    Bisol, Alexandra; Sion, E. M.

    2011-01-01

    We have selected three nova-like variables: V363 Aur, RZ Gru and AC Cnc, all of which are UX UMa types, having similar orbital periods well beyond the 3 to 4 hour range where most nova-likes are found. All should have very similar secondary stars given the fact that they their physical parameters are so similar. V363 Aur is a bona fide SW Sex star, and AC Cnc is a probable one, while RZ Gru is not a member of the SW Sex subclass. Our objective is to carry out the first synthetic spectral analysis of far ultraviolet spectra of the three systems using state-of-the-art models both of accretion disks and photospheres. Therefore we shall compare the distances we obtain from the best fitting synthetic spectral models to other distance estimates in the literature. We present model-derived accretion rates and distances for all three systems. The FUV flux range of RZ Gru and V363 Aur is dominated by radiation from an optically thick, steady state, accretion but for AC Cnc, we find that a hot white dwarf accounts for 70% of the FUV flux. We compare the FUV characteristics and physical properties of these three long period nova-like systems to the properties of other nova-likes at shorter periods. This work was supported in part by NSF grant AST0807892 to Villanova University.

  14. Spectroscopic mode identification in gamma Doradus stars

    NASA Astrophysics Data System (ADS)

    Rylvia Pollard, Karen

    2015-08-01

    The MUSICIAN programme at the University of Canterbury has been successfully identifying frequencies and pulsation modes in many gamma Doradus stars using hundreds of precise, high resolution spectroscopic observations. This paper describes some of these frequency and mode identifications and the emerging patterns of the programme.

  15. The nova-like cataclysmic variable star: KUV 0859+415

    NASA Astrophysics Data System (ADS)

    Grauer, Albert D.; Ringwald, F. A.; Wegner, Gary; Liebert, James; Schmidt, Gary D.; Green, Richard F.

    1994-07-01

    KUV 0859+415 has been found to be an eclipsing, nova-like cataclysmic variable with an orbital period of 3 h and 40 min. We find that it differs from other eclipsing systems of similar period (sometimes called the SW Sex stars) in several important respects. First, the eclipses are shallow, V-shaped dips, suggesting that the inclination angle is relatively low. Also, while the excitation of the emission lines are relatively high (He II comparable to H-beta), they are relatively weak compared to the continuum. The high Balmer lines have broad absorption wings, characteristic of an optically thick accretion disk. Yet there is evidence for a 'hot spot' in the system, which reaches peak brightness near phase 0.9 before the eclipse. Perhaps the most puzzling property of this system is that the H-alpha emission line radial velocity leads rather than lags the expected position for the location of the accretion disk. We present briefly a phenomenological model for the system which has a hot spot located at the normal location for the accretion stream to first impact the disk and is the source of excess optical continuum. However, the velocity curve of the emission lines requires us to conclude that the bulk of this radiation forms on the opposite side of the disk. This also explains the fact that the peak equivalent widths of H-alpha occur at phases 0.4-0.5.

  16. Photometry of the SW Sextantis-type nova-like BH Lyncis in high state

    NASA Astrophysics Data System (ADS)

    Stanishev, V.; Kraicheva, Z.; Genkov, V.

    2006-08-01

    Aims.We present a photometric study of the deeply eclipsing SW Sex-type nova-like cataclysmic variable star BH Lyn. Methods: .Time-resolved V-band CCD photometry was obtained for seven nights between 1999 and 2004. Results: .We determined 11 new eclipse timings of BH Lyn and derived a refined orbital ephemeris with an orbital period of 0.155875577(14) °. During the observations, BH Lyn was in high-state with V≃15.5 mag. The star presents ~1.5 mag deep eclipses with mean full-width at half-flux of 0.0683(±0.0054)P_orb. The eclipse shape is highly variable, even changing form cycle to cycle. This is most likely due to accretion disc surface brightness distribution variations, most probably caused by strong flickering. Time-dependent accretion disc self-occultation or variations of the hot spot(s) intensity are also possible explanations. Negative superhumps with period of ˜0.145 ° are detected in two long runs in 2000. A possible connection between SW Sex and negative superhump phenomena through the presence of tilted accretion disc is discussed, and a way to observationally test this is suggested.

  17. Unusual ``Stunted'' Outbursts in Old Novae and Nova-Like Cataclysmic Variables

    NASA Astrophysics Data System (ADS)

    Honeycutt, R. K.; Robertson, J. W.; Turner, G. W.

    1998-06-01

    Outbursts averaging 0.6 mag in amplitude and 10 days in width are described in five old novae and nova-like cataclysmic variables: UU Aqr, Q Cyg, CP Lac, X Ser, and RW Sex. These stars are thought to be high mass transfer rate systems for which the accretion disk is expected to be stable against the thermal instability responsible for dwarf nova outbursts. The widths and spacings of these events are similar to those of dwarf nova eruptions, but the amplitudes are significantly smaller, or ``stunted.'' The outbursts are sometimes accompanied by dips. These dips have amplitudes that are similar to the outbursts' but have shapes that scatter significantly more than the shapes of the outbursts. The outbursts and dips sometimes occur as pairs and are sometimes isolated. We are not able at this time to determine a single common mechanism for this behavior, or even to conclude that some mechanisms are preferred. Rather, we characterize these phenomena with regard to outburst shapes and frequency of occurrence and explore a range of possible causes, including truncated disks, mass transfer modulations, and Z Camelopardalis type behavior. Arguments are assembled for and against such possible mechanisms, and key observations are suggested. It appears unlikely that accretion disk instabilities are the single common cause of these phenomena, and we are left with either a combination of accretion disk and mass transfer events or a situation in which mass transfer events are somehow responsible for all these varied behaviors.

  18. The nova-like variable KQ Mon and the nature of the UX Ursa Majoris stars

    NASA Technical Reports Server (NTRS)

    Sion, E. M.; Guinan, E. F.

    1982-01-01

    The KQ Mon is a UX UMa type nova-like variable discovered by Howard Bond. Optical spectra taken by Bond in 1978 reveal very shallow Balmer absorption lines and He I absorption. Bond also did UBV and high speed photometry in 1978 and early 1981. There has been no evidence of orbital variations but the appearance of the optical spectrum and the presence of low amplitude flickering suggested a strong similarity to CD-42-14462 (=V3885 Sgr) and other members of the UX UMa class. Low dispersion observations of KQ Mon were made with the International Ultraviolet Explorer satellite. Six spectra taken with the shot wavelength prime camera are dominated by strong broad absorption lines due to N V, O I, Si III, Si IV, C IV, He II, N IV, and Al III. There is little evidence of orbital phase modulation over the time baseline of the observations. Unlike UV observations of other UX UMa type objects, KQ Mon exhibits no emission lines or P Cygni type profiles and the velocity displacements appear to be smaller, suggesting the absence of a hot, high velocity wind characterizing other UX UMa stars. The relationship of KQ Mon to other UX UMa disk stars is discussed and a model is suggested to explain their observed properties and the lack of major outbursts.

  19. Submillimeter Observations of the 30 Doradus Nebula

    NASA Astrophysics Data System (ADS)

    Kim, S.

    2007-06-01

    We present a mid-J CO emission line study of the largest H II complex, the 30 Doradus nebula in the Large Magellanic Cloud (LMC). This is the most luminous example of a starburst region in the Local Group. We have searched for 12CO J=7→6 emission towards the 30 Doradus complex with the Antarctic Submillimeter Telescope and Remote Observatory (AST/RO), located at 2847 m above mean sea level at the Amundsen-Scott South Pole Station. As a result we have detected a 12CO J=7→6 emitting cloud near the 30 Doradus complex. The 12CO J=7→6/12CO J=4→3 line temperature ratio in this region is approximately a factor of two higher than that observed near the Sgr B2 complex. A radiative transfer calculation using the line ratios shows that the core of massive star formation in the LMC is much warmer and denser than that of the Milky Way region.

  20. Hubble Space Telescope Eclipse Observations of the Nova Like Cataclysmic Variable UX Ursae Majoris

    NASA Technical Reports Server (NTRS)

    Knigge, Christian; Long, Knox S.; Wade, Richard A.; Baptista, Raymundo; Horne, Keith; Hubeny, Ivan; Rutten, Rene G. M.

    1998-01-01

    We present and analyze Hubble Space Telescope observations of the eclipsing nova-like cataclysmic variable UX UMa obtained with the Faint Object Spectrograph. Two eclipses each were observed with the G160L grating (covering the ultraviolet waveband) in 1994 August and with the PRISM (covering the near-ultraviolet to near-infrared) in November of the same year. The system was about 50% brighter in November than in August, which, if due to a change in the accretion rate, indicates a fairly substantial increase in Mass accretion by about 50%. The eclipse light curves are qualitatively consistent with the gradual occultation of an accretion disk with a radially decreasing temperature distribution. The light curves also exhibit asymmetries about mideclipse that are likely due to a bright spot at the disk edge. Bright-spot spectra have been constructed by differencing the mean spectra observed at pre- and posteclipse orbital phases. These difference spectra contain ultraviolet absorption lines and show the Balmer jump in emission. This suggests that part of the bright spot may be optically thin in the continuum and vertically extended enough to veil the inner disk and/or the outflow from UX UMa in some spectral lines. Model disk spectra constructed as ensembles of stellar atmospheres provide poor descriptions of the observed posteclipse spectra, despite the fact that UX UMa's light should be dominated by the disk at this time. Suitably scaled single temperature model stellar atmospheres with T(sub eff) approximately equals 12,500-14,500 K actually provide a better match to both the ultraviolet and optical posteclipse spectra. Evidently, great care must be taken in attempts to derive accretion rates from comparisons of disk models to observations. One way to reconcile disk models with the observed posteclipse spectra is to postulate the presence of a significant amount of optically thin material in the system. Such an optically thin component might be associated with the

  1. Infrared spectroscopy of cataclysmic variables - III. Dwarf novae below the period gap and nova-like variables

    NASA Astrophysics Data System (ADS)

    Dhillon, V. S.; Littlefair, S. P.; Howell, S. B.; Ciardi, D. R.; Harrop-Allin, M. K.; Marsh, T. R.

    2000-06-01

    We present K-band spectra of the short-period dwarf novae YZ Cnc, LY Hya, BK Lyn, T Leo, SW UMa and WZ Sge, the nova-like variables DW UMa, V1315 Aql, RW Tri, VY Scl, UU Aqr and GP Com, and a series of field dwarf stars with spectral types ranging from K2 to M6. The spectra of the dwarf novae are dominated by emission lines of Hi and Hei. The large velocity and equivalent widths of these lines, in conjunction with the fact that the lines are double-peaked in the highest inclination systems, indicate an accretion disc origin. In the case of YZ Cnc and T Leo, for which we obtained time-resolved data covering a complete orbital cycle, the emission lines show modulations in their equivalent widths that are most probably associated with the bright spot (the region where the gas stream collides with the accretion disc). There are no clear detections of the secondary star in any of the dwarf novae below the period gap, yielding upper limits of 10-30per cent for the contribution of the secondary star to the observed K-band flux. In conjunction with the K-band magnitudes of the dwarf novae, we use the derived secondary star contributions to calculate lower limits to the distances to these systems. The spectra of the nova-like variables are dominated by broad, single-peaked emission lines of Hi and Hei - even the eclipsing systems we observed do not show the double-peaked profiles predicted by standard accretion disc theory. With the exception of RW Tri, which exhibits Nai, Cai and 12CO absorption features consistent with a M0V secondary contributing 65per cent of the observed K-band flux, we find no evidence for the secondary star in any of the nova-like variables. The implications of this result are discussed.

  2. Internal rotation of γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Ouazzani, R.-M.; Salmon, S. J. A. J.; Antoci, V.; Bedding, T. R.; Murphy, S. J.

    2016-12-01

    Thanks to the exquisite Kepler data, resulting from four years of quasi-continuous photometric observations, we are now able to use g-modes in order to reveal the internal structure of γ Doradus stars. In particular, it is now possible to detect series of g-modes with non-uniform period spacing, which carry the signature of internal rotation. In a theoretical work published earlier this year, we have defined a new seismic diagnostic for rotation in the γ Doradus stars that are rotating too rapidly to present rotational splitting. It is based on a new observable that is the slope of the period spacing when plotted againt the period. Here we recall the one-to-one relation between this observable and the internal rotation rate. We explain how it can be used without any additional constraint in order to retreive the rotation rate in the cavity probed by the observed g-modes. Finally we evaluate the uncertainty induced by the use of the asymptotic formulation of the traditional approximation, and we give a word of caution concerning retrograde modes.

  3. THE GEOMETRY AND IONIZATION STRUCTURE OF THE WIND IN THE ECLIPSING NOVA-LIKE VARIABLES RW TRI AND UX UMa

    SciTech Connect

    Noebauer, Ulrich M.; Long, Knox S.; Sim, Stuart A.; Knigge, Christian

    2010-08-20

    The UV spectra of nova-like variables are dominated by emission from the accretion disk, modified by scattering in a wind emanating from the disk. Here, we model the spectra of RW Tri and UX UMa, the only two eclipsing nova-like variables which have been observed with the Hubble Space Telescope in the far-ultraviolet, in an attempt to constrain the geometry and the ionization structure of their winds. Using our Monte Carlo radiative transfer code, we computed spectra for simply parameterized axisymmetric biconical outflow models and were able to find plausible models for both systems. These reproduce the primary UV resonance lines-N V, Si IV, and C IV-in the observed spectra in and out of eclipse. The distribution of these ions in the wind models is similar in both cases as is the extent of the primary scattering regions in which these lines are formed. The inferred mass-loss rates are 6%-8% of the mass accretion rates for the systems. We discuss the implication of our point models for our understanding of accretion disk winds in cataclysmic variables.

  4. BK Lyn: A Unique Nova-Like Variable Below The Period Gap with a Hot White Dwarf

    NASA Astrophysics Data System (ADS)

    Ballouz, Ronald-Louis; Sion, E. M.; Gaensicke, B. T.; Long, K. S.

    2009-01-01

    BK Lyn is a nova-like variable observed with HST as part of a multi-HST cycle CV snapshot survey (B. Gaensicke, PI) which was only partially completed due to the failure of STIS. With an orbital period of 108 minutes, BK Lyn is the only bona fide nova-like variable lying below the CV period gap. Using the method of Knigge (2006, MNRAS, 373, 484), its distance lies within the range 116 to 291 pc. Optically thick accretion disk models yield distances in excess of 1.5 kpc. We have found good synthetic spectral fits with white dwarf model photospheres having T$_{eff}$ between 38,000$K$ and 34,000$K$, log $g$ between 7 and 8.6 and model-derived distances of 137 and 307 pc, respectively. Both distances are in reasonable agreement with the Knigge distance range. The white dwarf may be the hottest CV primary below the period gap. The evolutionary significance and implications of BK Lyn are discussed. This work is supported by NSF grant AST-0807892 and NASA/HST grant GO-9724.02A, both to Villanova University.

  5. The hot γ Doradus and Maia stars

    NASA Astrophysics Data System (ADS)

    Balona, L. A.; Engelbrecht, C. A.; Joshi, Y. C.; Joshi, S.; Sharma, K.; Semenko, E.; Pandey, G.; Chakradhari, N. K.; Mkrtichian, David; Hema, B. P.; Nemec, J. M.

    2016-08-01

    The hot γ Doradus stars have multiple low frequencies characteristic of γ Dor or SPB variables, but are located between the red edge of the SPB and the blue edge of the γ Dor instability strips where all low-frequency modes are stable in current models of these stars. Though δ Sct stars also have low frequencies, there is no sign of high frequencies in hot γ Dor stars. We obtained spectra to refine the locations of some of these stars in the H-R diagram and conclude that these are, indeed, anomalous pulsating stars. The Maia variables have multiple high frequencies characteristic of β Cep and δ Sct stars, but lie between the red edge of the β Cep and the blue edge of the δ Sct instability strips. We compile a list of all Maia candidates and obtain spectra of two of these stars. Again, it seems likely that these are anomalous pulsating stars which are currently not understood.

  6. FAR-ULTRAVIOLET SPECTROSCOPY OF THE NOVA-LIKE VARIABLE KQ MONOCEROTIS: A NEW SW SEXTANTIS STAR?

    SciTech Connect

    Wolfe, Aaron; Sion, Edward M.; Bond, Howard E. E-mail: edward.sion@villanova.edu

    2013-06-01

    New optical spectra obtained with the SMARTS 1.5 m telescope and archival International Ultraviolet Explorer (IUE) far-ultraviolet (FUV) spectra of the nova-like variable KQ Mon are discussed. The optical spectra reveal Balmer lines in absorption as well as He I absorption superposed on a blue continuum. The 2011 optical spectrum is similar to the KPNO 2.1 m IIDS spectrum we obtained 33 years earlier except that the Balmer and He I absorption is stronger in 2011. Far-ultraviolet IUE spectra reveal deep absorption lines due to C II, Si III, Si IV, C IV, and He II, but no P Cygni profiles indicative of wind outflow. We present the results of the first synthetic spectral analysis of the IUE archival spectra of KQ Mon with realistic optically thick, steady-state, viscous accretion-disk models with vertical structure and high-gravity photosphere models. We find that the photosphere of the white dwarf (WD) contributes very little FUV flux to the spectrum and is overwhelmed by the accretion light of a steady disk. Disk models corresponding to a WD mass of {approx}0.6 M {sub Sun }, with an accretion rate of order 10{sup -9} M {sub Sun} yr{sup -1} and disk inclinations between 60 Degree-Sign and 75 Degree-Sign , yield distances from the normalization in the range of 144-165 pc. KQ Mon is discussed with respect to other nova-like variables. Its spectroscopic similarity to the FUV spectra of three definite SW Sex stars suggests that it is likely a member of the SW Sex class and lends support to the possibility that the WD is magnetic.

  7. MOLECULAR AND IONIZED HYDROGEN IN 30 DORADUS. I. IMAGING OBSERVATIONS

    SciTech Connect

    Yeh, Sherry C. C.; Seaquist, Ernest R.; Matzner, Christopher D.; Pellegrini, Eric W.

    2015-07-10

    We present the first fully calibrated H{sub 2} 1–0 S(1) image of the entire 30 Doradus nebula. The observations were conducted using the NOAO Extremely Wide-field Infrared Imager (NEWFIRM) on the CTIO 4 m Blanco Telescope. Together with a NEWFIRM Brγ image of 30 Doradus, our data reveal the morphologies of the warm molecular gas and ionized gas in 30 Doradus. The brightest H{sub 2}-emitting area, which extends from the northeast to the southwest of R136, is a photodissociation region (PDR) viewed face-on, while many clumps and pillar features located at the outer shells of 30 Doradus are PDRs viewed edge-on. Based on the morphologies of H{sub 2}, Brγ, CO, and 8 μm emission, the H{sub 2} to Brγ line ratio, and Cloudy models, we find that the H{sub 2} emission is formed inside the PDRs of 30 Doradus, 2–3 pc to the ionization front of the H ii region, in a relatively low-density environment <10{sup 4} cm{sup −3}. Comparisons with Brγ, 8 μm, and CO emission indicate that H{sub 2} emission is due to fluorescence, and provide no evidence for shock excited emission of this line.

  8. Hubble Space Telescope STIS Spectroscopy of the Peculiar Nova-Like Variables BK Lyn, V751 Cygni, and V380 Oph

    NASA Astrophysics Data System (ADS)

    Zellem, Robert; Hollon, Nicholas; Ballouz, Ronald-Louis; Sion, Edward M.; Godon, Patrick; Gänsicke, Boris T.; Long, Knox

    2009-09-01

    We obtained Hubble STIS spectra of three nova-like variables: V751 Cygni, V380 Oph, and—the only confirmed nova-like variable known to be below the period gap—BK Lyn. In all three systems, the spectra were taken during high optical brightness state, and a luminous accretion disk dominates their far-ultraviolet (FUV) light. We assessed a lower limit of the distances by applying the infrared photometric method of Knigge. Within the limitations imposed by the poorly known system parameters (such as the inclination, white dwarf mass, and the applicability of steady state accretion disks) we obtained satisfactory fits to BK Lyn using optically thick accretion disk models with an accretion rate of for a white dwarf mass of Mwd = 1.2M⊙ and for Mwd = 0.4M⊙. However, for the VY Scl-type nova-like variable V751 Cygni and for the SW Sex star V380 Oph, we are unable to obtain satisfactory synthetic spectral fits to the high state FUV spectra using optically thick steady state accretion disk models. The lack of FUV spectra information down to the Lyman limit hinders the extraction of information about the accreting white dwarf during the high states of these nova-like systems.

  9. First NIR Polarimetry of 30 Doradus

    NASA Astrophysics Data System (ADS)

    Nakajima, Yasushi; Kandori, Ryo; Tamura, Motohide; Kusakabe, Nobuhiko; Hashimoto, Jun; Nagayama, Takahiro; Nagata, Tetsuya; Hatano, Hirofumi; Kato, Daisuke; Hough, James H.

    2007-06-01

    We have carried out the first near-infrared (NIR) polarimetric imaging observations of the central part (7'.7 × 7'.7 ˜ 110 pc} × 110 pc of 30 Doradus. The totals of 106, 131, and 34 sources have significant polarization degrees at J, H, and Ks, respectively. The E-vectors toward 30 Dor were mapped with unprecedentedly high resolutions. As previously noticed by optical observations, there is an overall tendency for the E-vectors to be roughly aligned in the east-west direction. In addition, our observations reveal a more complex structure of their alignment. The position angles of the E-vectors are not uniform, but show a gradual spatial variation. It is plausible to assume that they trace the magnetic field toward 30 Dor. One of the expanding shells toward 30 Dor, Shell 5, is clearly enveloped by E-vectors. The expanding shell has probably swept the magnetic field together into the rim, while creating the curved structure. We obtained the ratios of polarization degree among the NIR wavelengths: PH/PJ = 0.76±0.02 and PKs/PH = 0.87±0.06. These fit a power-law dependence of P ∝ λ-0.9.

  10. Photometry and Spectroscopy of 11 γ Doradus Stars

    NASA Astrophysics Data System (ADS)

    Henry, Gregory W.; Fekel, Francis C.; Henry, Stephen M.

    2007-04-01

    We have used precise photometric and high-dispersion spectroscopic observations to study 11 γ Doradus stars, 10 of them newly confirmed. Only five of these 11 γ Doradus stars appear to be single; two are primaries of double-lined spectroscopic binaries, one is the secondary of a double-lined binary, two are primaries of visual binaries and, in the case of the double-lined binary (HD 86371), either or both components could be a pulsating γ Doradus star. We have determined a preliminary orbital period of 5.32 days for the double-lined binary HD 41547. Several of the stars show spectroscopic line-profile and low-amplitude radial velocity variability indicative of pulsation. All 11 stars are photometrically variable with amplitudes between 4 and 94 mmag in Johnson B and periods between 0.38 and 1.86 days. The 11 stars have between two and five independent periods. The variability at all periods approximates a sinusoid. We provide a new tabulation of all 66 γ Doradus stars confirmed to date and list some of their properties. All are dwarfs or subgiants and lie within a well-defined region of the H-R diagram that overlaps the cool edge of the δ Scuti instability strip. Four of the new γ Doradus variables from this paper also lie within the δ Scuti instability strip but do not exhibit the additional higher frequency variability typical of δ Scuti stars. Among the 66 confirmed γ Doradus variables, we find no correlation between the period of the strongest pulsation mode and the (B - V) color index, absolute magnitude, or luminosity.

  11. 30 Doradus: The Low-Mass Stars

    NASA Astrophysics Data System (ADS)

    Zinnecker, H.; Brandl, B.; Brandner, W.; Moneti, A.; Hunter, D.

    We have obtained HST/NICMOS H-band images of the central 1'x1' field around the R136 starburst cluster in the 30 Doradus HII region, in an attempt to reveal the presence (or absence) of a low-mass stellar population (M < 1 Mo). We will discuss the fascinating prospect of 30 Dor/R136 being a proto-globular cluster and a template starburst unit. At the time of writing, we are still working to determine which method and photometry package is best suited to our 0.15" NICMOS images, which are characterised by extreme crowding in the cluster center and a peculiar and slightly undersampled NICMOS PSF. The main difficulty with the PSF is identifying the many "dots" that appear outside the Airy ring as PSF features and not as faint stars. Prelimininary analysis suggests that the H-band luminosity function rises at least until H = 20 (2 Mo). We have detected numerous stars with 20.0 < H < 22.5 (the latter corresponding to 0.4 Mo) beyond about 7" from the cluster centre, but we have not yet determined the completeness in that magnitude range, and we are not yet in a position to make a statement about the shape of the H-band luminosity function there. We have combined our infrared data with the optical WFPC2 images of Hunter et al. (1995) to produce a VIH 3-colour image of the central 30" x 30" area. The result clearly shows unexpected patches of extinction, with one patch only about 5" from the cluster core.

  12. DRIVING G-MODE PULSATIONS IN GAMMA DORADUS VARIABLES

    SciTech Connect

    J. GUZIK; A. KAYE; ET AL

    2000-10-10

    The {gamma} Doradus stars are a newly-discovered class of gravity-mode pulsators which lie just at or beyond the red edge of the {delta} Scuti instability strip. We present the results of calculations which predict pulsation instability of high-order g-modes with periods between 0.4 and 3 days, as observed in these stars. The pulsations are driven by the modulation of radiative flux by convection at the base of a deep envelope convection zone. Pulsation instability is predicted only for models with temperatures at the convection zone base between {approximately}200,000 and {approximately}480,000 K. The estimated shear dissipation due to turbulent viscosity within the convection zone, or in an overshoot region below the convection zone, can be comparable to or even exceed the predicted driving, and is likely to reduce the number of unstable modes, or possibly to quench the instability. Additional refinements in the pulsation modeling are required to determine the outcome. A few Doradus stars have been observed that also pulsate in {delta} Scuti-type p-modes, and at least two others have been identified as chemically peculiar. Since our calculated driving region is relatively deep, Doradus pulsations are not necessarily incompatible with surface abundance peculiarities or with {delta} Scuti p-mode pulsations driven by the H and He-ionization {kappa} effect. Such stars will provide useful observational constraints on the proposed Doradus pulsation mechanism.

  13. The nova-like nebular optical spectrum of V404 Cygni at the beginning of the 2015 outburst decay

    NASA Astrophysics Data System (ADS)

    Rahoui, Farid; Tomsick, J. A.; Gandhi, P.; Casella, P.; Fürst, F.; Natalucci, L.; Rossi, A.; Shaw, A. W.; Testa, V.; Walton, D. J.

    2017-03-01

    We report on FORS2 optical spectroscopy of the black hole X-ray binary V404 Cygni, performed at the very beginning of its 2015 outburst decay, complemented by quasi-simultaneous Swift X-ray and ultraviolet as well as Rapid Eye Mountain near-infrared observations. Its peculiar spectrum is dominated by a wealth of emission signatures of H I, He I, and higher ionization species, in particular Fe II. The spectral features are divided between broad redshifted and narrow stationary varieties, the latter being emitted in the outer regions. Continuum and line variability at short time-scale is high, and we find Baldwin effect-like anticorrelations between the full widths at half-maximum and equivalent widths of the broad lines with their local continua. The Balmer decrement H α/H β is also abnormally large at 4.61 ± 0.62. We argue that these properties hint at the broad lines being optically thick and arising within a circumbinary component in which shocks between faster optically thick and slower optically thin regions may occur. We associate it to a nova-like nebula formed by the cooling remnant of strong accretion disc winds that turned off when the mass-accretion rate dropped following the last major flare. The Fe II lines likely arise from the overlap region between this nebula and the companion star winds, whereas we favour the shocks within the nebula as responsible for the optical continuum via self-absorbed optically thin bremsstrahlung. The presence of a near-infrared excess also points towards the contribution of a strongly variable compact jet or a dusty component.

  14. RZ Leonis Minoris bridging between ER Ursae Majoris-type dwarf nova and nova-like system

    NASA Astrophysics Data System (ADS)

    Kato, Taichi; Ishioka, Ryoko; Isogai, Keisuke; Kimura, Mariko; Imada, Akira; Miller, Ian; Masumoto, Kazunari; Nishino, Hirochika; Kojiguchi, Naoto; Kawabata, Miho; Sakai, Daisuke; Sugiura, Yuki; Furukawa, Hisami; Yamamura, Kenta; Kobayashi, Hiroshi; Matsumoto, Katsura; Wang, Shiang-Yu; Chou, Yi; Ngeow, Chow-Choong; Chen, Wen-Ping; Panwar, Neelam; Lin, Chi-Sheng; Hsiao, Hsiang-Yao; Guo, Jhen-Kuei; Lin, Chien-Cheng; Omarov, Chingis; Kusakin, Anatoly; Krugov, Maxim; Starkey, Donn R.; Pavlenko, Elena P.; Antonyuk, Kirill A.; Sosnjvskij, Aleksei A.; Antonyuk, Oksana I.; Pit, Nikolai V.; Baklanov, Alex V.; Babina, Julia V.; Itoh, Hiroshi; Padovan, Stefano; Akazawa, Hidehiko; Kafka, Stella; de Miguel, Enrique; Pickard, Roger D.; Kiyota, Seiichiro; Shugarov, Sergey Yu.; Chochol, Drahomir; Krushevska, Viktoriia; Sekeráš, Matej; Pikalova, Olga; Sabo, Richard; Dubovsky, Pavol A.; Kudzej, Igor; Ulowetz, Joseph; Dvorak, Shawn; Stone, Geoff; Tordai, Tamás; Dubois, Franky; Logie, Ludwig; Rau, Steve; Vanaverbeke, Siegfried; Vanmunster, Tonny; Oksanen, Arto; Maeda, Yutaka; Kasai, Kiyoshi; Katysheva, Natalia; Morelle, Etienne; Neustroev, Vitaly V.; Sjoberg, George

    2016-12-01

    We observed RZ LMi, which is renowned for its extremely short (˜19 d) supercycle and is a member of a small, unusual class of cataclysmic variables called ER UMa-type dwarf novae, in 2013 and 2016. In 2016, the supercycles of this object substantially lengthened in comparison to the previous measurements to 35, 32, and 60 d for three consecutive superoutbursts. We consider that the object virtually experienced a transition to the nova-like state (permanent superhumper). This observed behavior reproduced the prediction of the thermal-tidal instability model extremely well. We detected a precursor in the 2016 superoutburst and detected growing (stage A) superhumps with a mean period of 0.0602(1) d in 2016 and in 2013. Combined with the period of superhumps immediately after the superoutburst, the mass ratio is not as small as in WZ Sge-type dwarf novae, having orbital periods similar to RZ LMi. By using least absolute shrinkage and selection operator (Lasso) two-dimensional power spectra, we detected possible negative superhumps with a period of 0.05710(1) d. We estimated an orbital period of 0.05792 d, which suggests a mass ratio of 0.105(5). This relatively large mass ratio is even above that of ordinary SU UMa-type dwarf novae, and it is also possible that the exceptionally high mass-transfer rate in RZ LMi may be a result of a stripped secondary with an evolved core in a system evolving toward an AM CVn-type object.

  15. X-Ray Observations of VY Scl-Type Nova-Like Binaries in the High and Low State

    NASA Technical Reports Server (NTRS)

    Zemko, P.; Orio, M.; Mukai, K.; Shugarov, S.

    2014-01-01

    Four VY Scl-type nova-like systems were observed in X-rays during both the low- and the high-optical states. We examined Chandra, ROSAT, Swift and Suzaku archival observations of BZ Cam, MV Lyr, TT Ari and V794 Aql. The X-ray flux of BZ Cam is higher during the low state, but there is no supersoft X-ray source (SSS) as hypothesized in previous articles. No SSS was detected in the low state of the any of the other systems, with the X-ray flux decreasing by a factor between 2 and 50. The best fit to the Swift X-ray spectra is obtained with a multicomponent model of plasma in collisional ionization equilibrium. The high-state high-resolution spectra of TT Ari taken with Chandra Advanced CCD Imaging Spectrometer (ACIS-S) and the Chandra High Energy Transmission Grating (HETG) shows a rich emission line spectrum, with prominent lines of Mg, Si, Ne and S. The complexity of this spectrum seems to have origin in more than one region, or more than one single physical mechanism. While several emission lines are consistent with a cooling flow in an accretion stream, there is at least an additional component. We discuss the origin of this component, which is probably arising in a wind from the system. We also examine the possibility that the VY Scl systems may be intermediate polars, and that while the boundary layer of the accretion disc emits only in the extreme ultraviolet, part of the X-ray flux may be due to magnetically driven accretion.

  16. Asteroseismology of hybrid δ Scuti-γ Doradus pulsating stars

    NASA Astrophysics Data System (ADS)

    Sánchez Arias, J. P.; Córsico, A. H.; Althaus, L. G.

    2017-01-01

    Context. Hybrid δ Scuti-γ Doradus pulsating stars show acoustic (p) oscillation modes typical of δ Scuti variable stars, and gravity (g) pulsation modes characteristic of γ Doradus variable stars simultaneously excited. Observations from space missions such as MOST, CoRoT, and Kepler have revealed a large number of hybrid δ Scuti-γ Doradus pulsators, thus paving the way for an exciting new channel of asteroseismic studies. Aims: We perform detailed asteroseismological modelling of five hybrid δ Scuti-γ Doradus stars. Methods: A grid-based modeling approach was employed to sound the internal structure of the target stars using stellar models ranging from the zero-age main sequence to the terminal-age main sequence, varying parameters such as stellar mass, effective temperature, metallicity and core overshooting. Their adiabatic radial (ℓ = 0) and non-radial (ℓ = 1,2,3) p and g mode periods were computed. Two model-fitting procedures were used to search for asteroseismological models that best reproduce the observed pulsation spectra of each target star. Results: We derive the fundamental parameters and the evolutionary status of five hybrid δ Scuti-γ Doradus variable stars recently observed by the CoRoT and Kepler space missions: CoRoT 105733033, CoRoT 100866999, KIC 11145123, KIC 9244992, and HD 49434. The asteroseismological model for each star results from different criteria of model selection, in which we take full advantage of the richness of periods that characterises the pulsation spectra for this kind of star.

  17. Optical, IUE, and ROSAT observations of the eclipsing nova-like variable V347 Puppis (LB 1800)

    NASA Technical Reports Server (NTRS)

    Mauche, Christopher W.; Raymond, John C.; Buckley, David A. H.; Mouchet, Martine; Bonnell, Jerry; Sullivan, Denis J.; Bonnet-Bidaud, Jean-Marc; Bunk, Wolfram H.

    1994-01-01

    Using time-resolved optical spectroscopy and UBVRI and high-speed photometry obtained at Mount Stromlo Observatory, Mount John University Observatory, and the South African Astronomical Observatory; International Ultraviolet Explorer (IUE) ultraviolet spectroscopy; and Roentgen Satellite (ROSAT) survey X-ray fluxes, we present a study of the accretion disk, hot spot, and emission line regions in the bright eclipsing nova-like variable V347 Pup (LB 1800). In the optical and UV, V347 Pup is a strong emission line source with a continuum spectrum which is remarkably red for a high-M cataclysmic variable. Consistent with its high inclination, we interpret the continuum spectrum as the superposition of the spectrum of the cool (T(sub eff) approximately 7000 K) outer edge and the hot (T(sub eff) approximately 100,000 K) inner regions of a self-eclipsed accretion disk. For the assumed parameters, the model matches the level and shape of the observed spectrum for an inclination of approximately 88 and a distance of approximately 300 pc. The prominent hump in the optical and UV light curves just before eclipse manifests the presence of the hot spot where the accretion stream strikes the edge of the disk. The wavelength dependence of the amplitude of the hump is best modeled by a spot having an effective temperature of approximately 25,000 K and an area of approximately 3 x 10(exp 18) sq cm if the spot radiates like a blackbody, or an effective temperatue of approximately 14,000 K and an area of approximately 3 x 10(exp 19) sq cm if it radiates with a stellar spectrum. In either case, the hot spot produces only one-tenth of the predicted luminosity for the assumed mass-transfer rate of 10(exp -8) solar mass/yr. Either the hot spot is 'buried' in the edge of the accretion disk, or a significant fraction of its luminosity is radiated away in lines. The difference in azimuth between the peak of the hump and the dynamically expected location of the hot spot suggests that the

  18. HST Spatially Resolved Spectra of the Accretion Disc and Gas Stream of the Nova-Like Variable UX Ursae Majoris

    NASA Technical Reports Server (NTRS)

    Baptista, Raymundo; Horne, Keith; Wade, Richard A.; Hubeny, Ivan; Long, Knox S.; Rutten, Rene G. M.

    1998-01-01

    Time-resolved eclipse spectroscopy of the nova-like variable UX UMa obtained with the Hubble Space Telescope/Faint Object Spectrograph (HST/FOS) on 1994 August and November is analysed with eclipse mapping techniques to produce spatially resolved spectra of its accretion disk and gas stream as a function of distance from the disk centre. The inner accretion disk is characterized by a blue continuum filled with absorption bands and lines, which cross over to emission with increasing disk radius, similar to that reported at optical wavelengths. The comparison of spatially resolved spectra at different azimuths reveals a significant asymmetry in the disk emission at ultraviolet (UV) wavelengths, with the disk side closest to the secondary star showing pronounced absorption by an 'iron curtain' and a Balmer jump in absorption. These results suggest the existence of an absorbing ring of cold gas whose density and/or vertical scale increase with disk radius. The spectrum of the infalling gas stream is noticeably different from the disc spectrum at the same radius suggesting that gas overflows through the impact point at the disk rim and continues along the stream trajectory, producing distinct emission down to 0.1 R(sub LI). The spectrum of the uneclipsed light shows prominent emission lines of Lyalpha, N v lambda1241, SiIV Lambda 1400, C IV Lambda 1550, HeII Lambda 1640, and MgII Lambda 2800, and a UV continuum rising towards longer wavelengths. The Balmer jump appears clearly in emission indicating that the uneclipsed light has an important contribution from optically thin gas. The lines and optically thin continuum emission are most probably emitted in a vertically extended disk chromosphere + wind. The radial temperature profiles of the continuum maps are well described by a steady-state disc model in the inner and intermediate disk regions (R greater than or equal to 0.3R(sub LI) ). There is evidence of an increase in the mass accretion rate from August to November

  19. Spectroscopic survey of γ Doradus stars - I. Comprehensive atmospheric parameters and abundance analysis of γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Kahraman Aliçavuş, F.; Niemczura, E.; De Cat, P.; Soydugan, E.; Kołaczkowski, Z.; Ostrowski, J.; Telting, J. H.; Uytterhoeven, K.; Poretti, E.; Rainer, M.; Suárez, J. C.; Mantegazza, L.; Kilmartin, P.; Pollard, K. R.

    2016-05-01

    We present a spectroscopic survey of known and candidate γ Doradus stars. The high-resolution, high signal-to-noise spectra of 52 objects were collected by five different spectrographs. The spectral classification, atmospheric parameters (Teff, log g, ξ), vsin i and chemical composition of the stars were derived. The stellar spectral and luminosity classes were found between G0-A7 and IV-V, respectively. The initial values for Teff and log g were determined from the photometric indices and spectral energy distribution. Those parameters were improved by the analysis of hydrogen lines. The final values of Teff, log g and ξ were derived from the iron lines analysis. The Teff values were found between 6000 K and 7900 K, while log g values range from 3.8 to 4.5 dex. Chemical abundances and vsin i values were derived by the spectrum synthesis method. The vsin i values were found between 5 and 240 km s-1. The chemical abundance pattern of γ Doradus stars were compared with the pattern of non-pulsating stars. It turned out that there is no significant difference in abundance patterns between these two groups. Additionally, the relations between the atmospheric parameters and the pulsation quantities were checked. A strong correlation between the vsin i and the pulsation periods of γ Doradus variables was obtained. The accurate positions of the analysed stars in the Hertzsprung-Russell diagram have been shown. Most of our objects are located inside or close to the blue edge of the theoretical instability strip of γ Doradus.

  20. The 30 Doradus Nebula: An Imaging Study of Molecular and Ionized Hydrogen

    NASA Astrophysics Data System (ADS)

    Yeh, Sherry; Seaquist, E. R.; Matzner, C. D.

    2013-01-01

    We present the very first, fully calibrated H2 1--0 S(1) image of the entire 30 Doradus nebula. The observations were carried out using the NOAO Extremely Wide Field Infrared Imager on the CTIO 4-meter telescope. Together with a Brγ image of 30 Dor taken by NEWFIRM, our images reveal the morphologies of the warm molecular gas and ionized gas in 30 Doradus. Based on the morphologies of H2 and Brγ, line ratio H2 to Brγ, and Cloudy models, we found that the H2 emission is formed inside the photodissociation regions of 30 Doradus, very close to the surface in association with the ionization front of the HII region. We also suggest that the bright H2-emitting area, which expands from the northeast to the southwest of R136, is a photodissociation region viewed face-on, while many clumps and elephant trunk features located at the outer shells of 30 Doradus are also photodissociation regions viewed edge-on. The characteristic radiation to gas pressure ratio is evaluated at selected regions in 30 Doradus, and we conclude that radiation pressure is not the dominating force at the current phase of 30 Doradus, while the pressurization of stellar winds and the injection of photoevaporative flows are likely the major feedback mechanisms acting to reduce the observed ionization parameter in 30 Doradus.

  1. The ALMA View of Dense Molecular Gas in 30 Doradus

    NASA Astrophysics Data System (ADS)

    Bittle, Lauren E.; Indebetouw, Remy; Brogan, Crystal L.; Hunter, Todd R.; Leroy, Adam

    2017-01-01

    At a distance of 50 kpc, the 30 Doradus region within the Large Magellanic Cloud (LMC) hosts several sites of star formation including R136, a starburst region home to dozens of evolved O stars. The intense radiation from R136 creates an extreme environment for nearby star formation in such a low-metallicity, low mass galaxy. We have targeted a star-forming region ~15 pc away from R136 within 30 Doradus using the Atacama Large Millimeter/submillimeter Array (ALMA) to map the molecular gas to study the sites of star formation. We are conducting a clump-by-clump analysis of the intensities and line ratios of dense gas (HCO+, HCN, CS, H13CO+, H13CN) and diffuse gas (CO, 13CO, C18O) tracers at sub-parsec resolution. We identify and characterize ~100 molecular clumps within the region. With the observed molecular species, we aim to determine the physical conditions of each clump (e.g. size, internal turbulence, molecular abundance). We compare the intensities and line ratios to non-LTE Radex model grids of the excitation temperature, molecular column density, and volume density of the H2 collider to determine the physical excitation conditions within the clumps. We compare these properties of each clump to both associated and embedded star formation properties to quantify the relative importance of internal feedback from the star formation itself versus external feedback processes from R136 and determine which process dominates in this region.

  2. The Orbital Periods and Variability of the Nova-Like BK Lyncis (PG 0917+342) and the Dwarf Nova WW Ceti

    NASA Astrophysics Data System (ADS)

    Ringwald, F. A.; Thorstensen, J. R.; Honeycutt, R. K.; Robertson, J. W.; Smith, Robert Connon

    1995-12-01

    Long-term light curves of the cataclysmic variable BK Lyn (PG 0917+342) from the Indiana Automated CCD photometric telescope (``RoboScope'') and the Harvard College Observatory plate archive reveal no dwarf nova outbursts. Two radial velocity studies show its orbital period to be 107.97 +/- 0.07 minutes, confirming that it does have an orbital period shorter than the 2 -- 3-hour orbital period gap for cataclysmic variables. Whether this is a nova-like below the period gap or a dwarf nova with rare outbursts resembling WZ Sge is still unclear, but anomalously high angular momentum loss below the period gap may imply that magnetic stellar-wind braking still works below the period gap---but mass-losing secondary stars there are thought to be fully convective, and therefore should not have magnetic braking. If BK Lyn is a genuine nova-like beneath the period gap, it may provide evidence of magnetic activity occurring in the faintest M dwarfs, here in an interacting binary. A radial velocity study resolves a long-standing aliasing problem and shows that the orbital period of the dwarf nova WW Cet is 0.17578 +/- 0.00013 d (4.22 hours). Its long-term light curves from RoboScope, AAVSO, Harvard archive, and VSS, RASNZ observations are examined. WW Cet does not have the characteristic standstills of the Z Cam stars, but does wander in quiescence by well over one magnitude. This and the orbital period become of interest in the context of recent speculation (Livio M., Pringle J. E., 1994, ApJ, 427, 956) that the low states of the VY Scl stars may be produced by star spots moving over L_1, choking off the mass flow, and that dwarf novae with orbital periods between 3 and 4 hours may be rare because of this.

  3. The Music of the Stars : Spectroscopy of Pulsations in gamma Doradus Stars

    NASA Astrophysics Data System (ADS)

    Brunsden, Emily

    2013-05-01

    p>The mysteries of the interior structures of stars are being tackled with asteroseismology. The observable parameters of the surface pulsations of stars inform us of the interior characteristics of numerous classes of stars. The main-sequence gamma Doradus stars, just a little hotter than the Sun, offer the potential of determining stellar structure right down to the core. To determine the structural profile of a star, the observed frequencies and a full geometric description must be determined. This is only possible with long-term spectroscopic monitoring and careful analysis of the pulsation signature in spectral lines. This work seeks to identify the pulsational geometry of several gamma Doradus stars and to identify areas of improvement for current observation, analysis and modelling techniques. More than 4500 spectra were gathered on five stars for this purpose. For three stars a successful multi-frequency and mode identification solution was determined and significant progress has been made towards the understanding of a binary system involving a gamma Doradus star. A hybrid gamma Doradus/nbsp;delta Scuti pulsator was also intensely monitored and results from this work raise important questions about the classification of this type of star. Current analysis techniques were found to be fit-for-purpose for pure gamma Doradus stars, but stars with complexities such as hybrid pulsations and/or fast rotation require future development of the current models./p>

  4. 4-1BB chimeric antigen receptors.

    PubMed

    Campana, Dario; Schwarz, Herbert; Imai, Chihaya

    2014-01-01

    In addition to T-cell receptor signals, T lymphocytes require costimulatory signals for robust activation. Among these, those mediated by 4-1BB (CD137, TNFRSF9) are critical for tumor immunity. 4-1BB is expressed in T-cell receptor-activated lymphocytes as well as natural killer cells and other hematopoietic and nonhematopoietic cells. 4-1BB ligation induces a signaling cascade that results in cytokine production, expression of antiapoptotic molecules, and enhanced immune responses. In line with the described function of 4-1BB, its addition to CD3ζ chimeric antigen receptors (CARs) increases their capacity to provoke T-cell expansion and antitumor activity. The results of preclinical studies with 4-1BB CARs have been corroborated by encouraging results from clinical trials. Advantages and disadvantages of 4-1BB CARs versus CARs bearing other costimulatory components remain to be fully elucidated. In this review, we discuss the properties of 4-1BB, the design of 4-1BB CARs, and the function of T lymphocytes and natural killer cells expressing them.

  5. A Multitransition CO Study in the 30 Doradus Complex in the Large Magellanic Cloud

    NASA Astrophysics Data System (ADS)

    Kim, Sungeun

    2006-01-01

    We have made a multitransition CO study of the largest H II complex, the 30 Doradus nebula, in the Large Magellanic Cloud (LMC). This is the most luminous example of a starburst region in the Local Group. We have searched for 12CO J=7-->6 emission toward the 30 Doradus complex with the Antarctic Submillimeter Telescope and Remote Observatory (AST/RO), located at 2847 m altitude at the Amundsen-Scott South Pole Station. As a result, we have detected a 12CO J=7-->6 emitting cloud near the 30 Doradus complex. The 12CO J=7-->6/12CO J=4-->3 line temperature ratio in this region is approximately a factor of 2 higher than that observed near the Sgr B2 complex. A radiative transfer calculation using the line ratios shows that the core of massive star formation in the LMC is much warmer and denser than that of the Milky Way.

  6. Binary stars in the AB Doradus moving group

    NASA Astrophysics Data System (ADS)

    Azulay, R.; Guirado, J. C.; Marcaide, J. M.; Martí-Vidal, I.; Ros, E.

    2017-03-01

    We present a study of the radio emission and kinematics of a sample of stars belonging to the AB Doradus moving group through VLBI observations. The main aim of our study is to obtain precise estimates of the dynamical mass of young, low-mass stars, which in combination with photometric measurements provide precise benchmarks for calibrating pre-main-sequence (PMS) stellar evolutionary models. Previous studies show that model predictions are in disagreement with experimental results for masses below 1.2 M_{⊙}. Among the stars included in our study, we emphasize the results obtained in two of them: AB Dor B and HD 160934, from which we have measured both the relative and absolute orbital motion. Accordingly, we obtained precise estimates of the mass of the components of these binaries (ranging from 0.25 to 0.7 M_{⊙}). Comparisons of the dynamical masses with the prediction of PMS evolutionary models show that the models underpredict the dynamical masses of the binary components by 10-40%.

  7. Structure and Feedback in 30 Doradus. I. Observations

    NASA Astrophysics Data System (ADS)

    Pellegrini, E. W.; Baldwin, J. A.; Ferland, G. J.

    2010-11-01

    We have completed a new optical imaging and spectrophotometric survey of a 140 × 80 pc2 region of 30 Doradus centered on R136, covering key optical diagnostic emission lines including Hα, Hβ, Hγ, [O III] λλ4363, 4959, 5007, [N II] λλ6548, 6584, [S II] λλ6717, 6731 [S III] λ6312, and in some locations [S III] λ9069. We present maps of fluxes and intensity ratios for these lines, and catalogs of isolated ionizing stars, elephant-trunk pillars, and edge-on ionization fronts. The final science-quality spectroscopic data products are available to the public. Our analysis of the new data finds that, while stellar winds and supernovae undoubtedly produce shocks and are responsible for shaping the nebula, there are no global spectral signatures to indicate that shocks are currently an important source of ionization. We conclude that the considerable region covered by our survey is well described by photoionization from the central cluster where the ionizing continuum is dominated by the most massive O stars. We show that if 30 Dor were viewed at a cosmological distance, its integrated light would be dominated by its extensive regions of lower surface brightness rather than by the bright, eye-catching arcs.

  8. STRUCTURE AND FEEDBACK IN 30 DORADUS. I. OBSERVATIONS

    SciTech Connect

    Pellegrini, E. W.; Baldwin, J. A.; Ferland, G. J.

    2010-11-15

    We have completed a new optical imaging and spectrophotometric survey of a 140 x 80 pc{sup 2} region of 30 Doradus centered on R136, covering key optical diagnostic emission lines including H{alpha}, H{beta}, H{gamma}, [O III] {lambda}{lambda}4363, 4959, 5007, [N II] {lambda}{lambda}6548, 6584, [S II] {lambda}{lambda}6717, 6731 [S III] {lambda}6312, and in some locations [S III] {lambda}9069. We present maps of fluxes and intensity ratios for these lines, and catalogs of isolated ionizing stars, elephant-trunk pillars, and edge-on ionization fronts. The final science-quality spectroscopic data products are available to the public. Our analysis of the new data finds that, while stellar winds and supernovae undoubtedly produce shocks and are responsible for shaping the nebula, there are no global spectral signatures to indicate that shocks are currently an important source of ionization. We conclude that the considerable region covered by our survey is well described by photoionization from the central cluster where the ionizing continuum is dominated by the most massive O stars. We show that if 30 Dor were viewed at a cosmological distance, its integrated light would be dominated by its extensive regions of lower surface brightness rather than by the bright, eye-catching arcs.

  9. HUBBLE SPACE TELESCOPE (HST) IMAGERY OF THE 30 DORADUS NEBULA

    NASA Technical Reports Server (NTRS)

    1990-01-01

    Hubble Space Telescope (HST) images of the 30 Doradus Nebula show its remarkable cluster of tightly-packed young stars 160,000 light years from Earth in the large Magellanic cloud galaxy. Panel A is a portion of a image made with the HST Wide Field Planetary Camera (WFPC). WFPC photographed four adjoining sky regions simultaneously which are assembled in this mosaic. Panel B is an enlargement of the central portion of the HST image which was made in violet light. It shows the compact star cluster R136, which consists of very hot and massive young stars. The star images have bright cores that are only 0.1 arc seconds wide, allowing many more stars to be distinguished than in previous ground-based telescopic photos. Panel C is a photograph of the same region as Panel B, obtained with the Max Planck 2.2 meter telescope at the European Southern Observatory in Chile. The star images are 0.6 arc seconds wide. Panel D shows how computer processing of the HST image in Panel B has sharpened its

  10. The dynamics and structure of the ionized and neutral gas in the 30 Doradus nebula

    NASA Astrophysics Data System (ADS)

    White, N. J.

    1981-08-01

    A variety of new optical observations have been made over the bright core of the supermassive H II region 30 Doradus and the ionized filamentary material surrounding this object. In addition, a more detailed analysis of previously published interstellar absorption and H I emission profiles has been undertaken. The velocity and density structure of this complex region is discussed and the new results analyzed here shown to be compatible to the model of 30 Doradus presented in Canto et al. (1980) and Meaburn (1980).

  11. B.B. Contracting & Remodeling Information Sheet

    EPA Pesticide Factsheets

    B.B. Contracting & Remodeling (the Company) is located in St. Louis, Missouri. The settlement involves renovation activities conducted at property constructed prior to 1978, located in St. Louis, Missouri.

  12. Swift X-Ray Telescope Observations of the Nova-like Cataclysmic Variables MV Lyr, BZ Cam, and V592 Cas

    NASA Astrophysics Data System (ADS)

    Balman, Şölen; Godon, Patrick; Sion, Edward M.

    2014-10-01

    We present a total of ~45 ks (3 × 15 ks) of Swift X-Ray Telescope (XRT) observations for three nonmagnetic nova-like (NL) cataclysmic variables (CVs; MV Lyr, BZ Cam, V592 Cas) in order to study characteristics of boundary layers (BLs) in CVs. The nonmagnetic NLs are found mostly in a state of high mass accretion rate (>=1 × 10-9 M ⊙ yr-1), and some show occasional low states. Using the XRT data, we find optically thin multiple-temperature cooling flow type emission spectra with X-ray temperatures (kT max) of 21-50 keV. These hard X-ray-emitting BLs diverge from simple isobaric cooling flows, indicating X-ray temperatures that are of virial values in the disk. In addition, we detect power-law emission components from MV Lyr and BZ Cam and plausibly from V592 Cas, which may be a result of the Compton scattering of the optically thin emission from the fast wind outflows in these systems and/or Compton upscattering of the soft disk photons. The X-ray luminosities of the (multitemperature) thermal plasma emission in the 0.1-50.0 keV range are (0.9-5.0) × 1032 erg s-1. The ratio of the X-ray and disk luminosities (calculated from the UV-optical wavelengths) yields an efficiency (Lx /L disk) ~ 0.01-0.001. Given this non-radiative ratio for the X-ray-emitting BLs with no significant optically thick blackbody emission in the soft X-rays (consistent with ROSAT observations), together with the high/virial X-ray temperatures, we suggest that high-state NL systems may have optically thin BLs merged with ADAF-like flows and/or X-ray coronae. In addition, we note that the axisymmetric bipolar and/or rotation-dominated fast-wind outflows detected in these three NLs (particularly BZ Cam and V592 Cas) or some other NL may also be explained in the context of ADAF-like BL regions.

  13. Swift X-ray telescope observations of the nova-like cataclysmic variables MV Lyr, BZ Cam, and V592 Cas

    SciTech Connect

    Balman, Şölen; Godon, Patrick; Sion, Edward M. E-mail: patrick.godon@villanova.edu

    2014-10-10

    We present a total of ∼45 ks (3 × 15 ks) of Swift X-Ray Telescope (XRT) observations for three nonmagnetic nova-like (NL) cataclysmic variables (CVs; MV Lyr, BZ Cam, V592 Cas) in order to study characteristics of boundary layers (BLs) in CVs. The nonmagnetic NLs are found mostly in a state of high mass accretion rate (≥1 × 10{sup –9} M {sub ☉} yr{sup –1}), and some show occasional low states. Using the XRT data, we find optically thin multiple-temperature cooling flow type emission spectra with X-ray temperatures (kT {sub max}) of 21-50 keV. These hard X-ray-emitting BLs diverge from simple isobaric cooling flows, indicating X-ray temperatures that are of virial values in the disk. In addition, we detect power-law emission components from MV Lyr and BZ Cam and plausibly from V592 Cas, which may be a result of the Compton scattering of the optically thin emission from the fast wind outflows in these systems and/or Compton upscattering of the soft disk photons. The X-ray luminosities of the (multitemperature) thermal plasma emission in the 0.1-50.0 keV range are (0.9-5.0) × 10{sup 32} erg s{sup –1}. The ratio of the X-ray and disk luminosities (calculated from the UV-optical wavelengths) yields an efficiency (L{sub x} /L {sub disk}) ∼ 0.01-0.001. Given this non-radiative ratio for the X-ray-emitting BLs with no significant optically thick blackbody emission in the soft X-rays (consistent with ROSAT observations), together with the high/virial X-ray temperatures, we suggest that high-state NL systems may have optically thin BLs merged with ADAF-like flows and/or X-ray coronae. In addition, we note that the axisymmetric bipolar and/or rotation-dominated fast-wind outflows detected in these three NLs (particularly BZ Cam and V592 Cas) or some other NL may also be explained in the context of ADAF-like BL regions.

  14. Drei neue gamma-Doradus-Sterne aus der ASAS-3 Datenbank

    NASA Astrophysics Data System (ADS)

    Bernhard, Klaus; Huemmerich, Stefan

    2016-02-01

    By analysis of data from the ASAS-3 archive, the stars HD 18011, NSV 16873 and NSV 3272 were identified as multiperiodic gamma Doradus variables. Essential information on these variables is presented, along with unwhitened frequency spectra and statistically significant frequencies, as derived with Period 04.

  15. A new asteroseismic diagnostic for internal rotation in γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Ouazzani, Rhita-Maria; Salmon, S. J. A. J.; Antoci, V.; Bedding, T. R.; Murphy, S. J.; Roxburgh, I. W.

    2017-02-01

    With four years of nearly continuous photometry from Kepler, we are finally in a good position to apply asteroseismology to γ Doradus stars. In particular, several analyses have demonstrated the possibility to detect non-uniform period spacings, which have been predicted to be directly related to rotation. In this paper, we define a new seismic diagnostic for rotation in γ Doradus stars which are too rapidly rotating to present rotational splittings. Based on the non-uniformity of their period spacings, we define the observable Σ as the slope of the period spacing when plotted as a function of period. We provide a one-to-one relation between this observable Σ and the internal rotation, which applies widely in the instability strip of γ Doradus stars. We apply the diagnostic to a handful of stars observed by Kepler. Thanks to g modes in γ Doradus stars, we are now able to determine the internal rotation of stars on the lower main sequence, which is still not possible for Sun-like stars.

  16. Spectroscopic mode identification of γ Doradus stars: frequencies, modes, rotation and wave leakage

    NASA Astrophysics Data System (ADS)

    Pollard, Karen R.; Brunsden, E.; Davie, M.; Greenwood, A.; Cottrell, P. L.

    The gravity modes present in γ Doradus stars probe the deep stellar interiors and are thus of particular interest in asteroseismology. The MUSICIAN programme at the University of Canterbury has been successfully identifying frequencies and pulsation modes in many γ Doradus stars using hundreds of precise, high resolution spectroscopic observations obtained with the 1.0 m telescope and HERCULES spectrograph at the Mt John Observatory in New Zealand. In this paper we present a summary of our spectroscopic frequency and mode identifications. Of particular interest from our spectroscopic analyses are: the prevalence of (l, m) = 1, 1 modes in many γ Dor stars; the importance of stellar rotation in the interpretation of the frequency and mode identification; and finally, possible evidence of wave leakage in one of these stars.

  17. Turbulent convection and pulsation stability of stars - II. Theoretical instability strip for δ Scuti and γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Xiong, D. R.; Deng, L.; Zhang, C.; Wang, K.

    2016-04-01

    By using a non-local and time-dependent convection theory, we have calculated radial and low-degree non-radial oscillations for stellar evolutionary models with M = 1.4-3.0 M⊙. The results of our study predict theoretical instability strips for δ Scuti and γ Doradus stars, which overlap with each other. The strip of γ Doradus is slightly redder in colour than that of δ Scuti. We have paid great attention to the excitation and stabilization mechanisms for these two types of oscillations, and we conclude that radiative κ mechanism plays a major role in the excitation of warm δ Scuti and γ Doradus stars, while the coupling between convection and oscillations is responsible for excitation and stabilization in cool stars. Generally speaking, turbulent pressure is an excitation of oscillations, especially in cool δ Scuti and γ Doradus stars and all cool Cepheid- and Mira-like stars. Turbulent thermal convection, on the other hand, is a damping mechanism against oscillations that actually plays the major role in giving rise to the red edge of the instability strip. Our study shows that oscillations of δ Scuti and γ Doradus stars are both due to the combination of κ mechanism and the coupling between convection and oscillations, and they belong to the same class of variables at the low-luminosity part of the Cepheid instability strip. Within the δ Scuti-γ Doradus instability strip, most of the pulsating variables are very likely hybrids that are excited in both p and g modes.

  18. Frequency analysis and pulsational mode identification of two γ Doradus stars: HD 40745 and HD 189631

    NASA Astrophysics Data System (ADS)

    Maisonneuve, F.; Pollard, K. R.; Cottrell, P. L.; Wright, D. J.; De Cat, P.; Mantegazza, L.; Kilmartin, P. M.; Suárez, J. C.; Rainer, M.; Poretti, E.

    2011-08-01

    Gravity modes present in γ Doradus stars probe the deep stellar interiors and are thus of particular interest in asteroseismology. Mode identification will improve the knowledge of these stars considerably and allow an understanding of the issues with current pulsational models. A frequency analysis followed by a mode identification were done based on the high-resolution spectroscopic data of two γ Doradus stars: HD 189631 and HD 40745. Extensive spectroscopic data sets are obtained by three instruments: HARPS, FEROS and HERCULES. We obtained 422 spectra for HD 189631 and 248 spectra for HD 40745. The pulsational frequencies were determined by four methods: analysis of the variation in equivalent width, variation in radial velocity, asymmetry of the line profile and the pixel-by-pixel frequency analysis. The mode identification was done using the recently developed Fourier Parameter Fit method. Without achieving the same degree of confidence for all results, we report the identification of four pulsational modes in HD 189631: (ℓ= 1; m =+1) at f1= 1.67 d-1; (3; -2) at f2= 1.42 d-1; (2; -2) at f3= 0.07 d-1; and (4; +1) at f4= 1.82 d-1; and two modes in HD 40745: (2; -1) at f1= 0.75 d-1 and (3; -3) at f2= 1.09 d-1. This study provides the first pulsational analysis based on spectroscopy of HD 189631 and HD 40745. We discuss the performance of current methods of analysis and outline the difficulties presented by γ Doradus stars. Based on observations made with the 1-m telescope at the Mount John University Observatory (HERCULES), and with ESO telescopes at the La Silla Observatories under the Normal Programme 081.D-0610 (HARPS) and the Large Programmes 178.D-0361 (FEROS) and 182.D-0356 (HARPS). Mode identification results were obtained with the software package FAMIAS developed in the framework of the FP6 European Coordination Action HELAS ().

  19. Spectroscopic pulsational frequency identification and mode determination of γ Doradus star HD 12901

    NASA Astrophysics Data System (ADS)

    Brunsden, E.; Pollard, K. R.; Cottrell, P. L.; Wright, D. J.; De Cat, P.

    2012-12-01

    Using multisite spectroscopic data collected from three sites, the frequencies and pulsational modes of the γ Doradus star HD 12901 were identified. A total of six frequencies in the range 1-2 d-1 were observed, their identifications supported by multiple line-profile measurement techniques and previously published photometry. Five frequencies were of sufficient signal-to-noise ratio for mode identification, and all five displayed similar three-bump standard deviation profiles which were fitted well with (l,m) = (1,1) modes. These fits had reduced χ2 values of less than 18. We propose that this star is an excellent candidate to test models of non-radially pulsating γ Doradus stars as a result of the presence of multiple (1,1) modes. This paper includes data taken at the Mount John University Observatory of the University of Canterbury (New Zealand), the McDonald Observatory of the University of Texas at Austin (Texas, USA) and the European Southern Observatory at La Silla (Chile).

  20. THE TUCANA/HOROLOGIUM, COLUMBA, AB DORADUS, AND ARGUS ASSOCIATIONS: NEW MEMBERS AND DUSTY DEBRIS DISKS

    SciTech Connect

    Zuckerman, B.; Rhee, Joseph H.; Song, Inseok; Bessell, M. S. E-mail: rhee@astro.ucla.edu E-mail: bessell@mso.anu.edu.au

    2011-05-10

    We propose 35 star systems within {approx}70 pc of Earth as newly identified members of nearby young stellar kinematic groups; these identifications include the first A- and late-B-type members of the AB Doradus moving group and field Argus Association. All but one of the 35 systems contain a bright solar- or earlier-type star that should make an excellent target for the next generation of adaptive optics (AO) imaging systems on large telescopes. AO imaging has revealed four massive planets in orbit around the {lambda} Boo star HR 8799. Initially, the planets were of uncertain mass due in large part to the uncertain age of the star. We find that HR 8799 is a likely member of the {approx}30 Myr old Columba Association, implying planet masses {approx}6 times that of Jupiter. We consider Spitzer Space Telescope MIPS photometry of stars in the {approx}30 Myr old Tucana/Horologium and Columba Associations, the {approx}40 Myr old field Argus Association, and the {approx}70 Myr old AB Doradus moving group. The percentage of stars in these young stellar groups that display excess emission above the stellar photosphere at 24 and 70 {mu}m wavelengths-indicative of the presence of a dusty debris disk-is compared with corresponding percentages for members of 11 open clusters and stellar associations with ages between 8 and 750 Myr, thus elucidating the decay of debris disks with time.

  1. STRUCTURE AND FEEDBACK IN 30 DORADUS. II. STRUCTURE AND CHEMICAL ABUNDANCES

    SciTech Connect

    Pellegrini, E. W.; Baldwin, J. A.; Ferland, G. J.

    2011-09-01

    We use our new optical-imaging and spectrophotometric survey of key diagnostic emission lines in 30 Doradus, together with CLOUDY photoionization models, to study the physical conditions and ionization mechanisms along over 4000 individual lines of sight at points spread across the face of the extended nebula, out to a projected radius 75 pc from R136 at the center of the ionizing cluster NGC 2070. We focus on the physical conditions, geometry, and importance of radiation pressure on a point-by-point basis, with the aim of setting observational constraints on important feedback processes. We find that the dynamics and large-scale structure of 30 Dor are set by a confined system of X-ray bubbles in rough pressure equilibrium with each other and with the confining molecular gas. Although the warm (10,000 K) gas is photoionized by the massive young stars in NGC 2070, the radiation pressure does not currently play a major role in shaping the overall structure. The completeness of our survey also allows us to create a composite spectrum of 30 Doradus, simulating the observable spectrum of a spatially unresolved, distant giant extragalactic H II region. We find that the highly simplified models used in the 'strong line' abundance technique do in fact reproduce our observed line strengths and deduced chemical abundances, in spite of the more than one order of magnitude range in the ionization parameter and density of the actual gas in 30 Dor.

  2. Floor Plans: Section "AA", Section "BB"; Floor Framing Plans: Section ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Floor Plans: Section "A-A", Section "B-B"; Floor Framing Plans: Section "A-A", Section "B-B" - Fort Washington, Fort Washington Light, Northeast side of Potomac River at Fort Washington Park, Fort Washington, Prince George's County, MD

  3. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart BB A Appendix A to Subpart BB of Part 63 Protection of... Hazardous Air Pollutants From Phosphate Fertilizers Production Plants Pt. 63, Subpart BB, App. A Appendix A to Subpart BB of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to...

  4. HR 7920: a very bright new Delta Scuti star with possible Gamma Doradus variations

    NASA Astrophysics Data System (ADS)

    Koen, C.; van Wyk, F.; Laney, C. D.; Kilkenny, D.

    2017-04-01

    We present photometric and high-dispersion spectroscopic measurements that show HR 7920 is a periodic variable. The photometry reveals at least four frequencies higher that 10 d-1, two of which are also probably present in the radial velocity variations. The frequencies are in a range typical of δ Scuti star pulsations. A further low frequency of about 2.8 d-1 may be present in both radial velocities and photometry; if real, this points to γ Doradus variability, which would make HR 7920 a hybrid pulsator. An attempt is made to identify the modes of the δ Scuti pulsations, which include both radial and non-radial modes. A new rotational velocity of 75 km s-1 is derived from co-added spectra, contrasting with published values in the range 128-150 km s-1.

  5. A procedure for modelling asymptotic g-mode pulsators: The case of γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Moya, A.; Suárez, J. C.; Martín-Ruiz, S.; Amado, P. J.; Rodríguez-López, C.; Grigahcène, A.; Dupret, M. A.; Rodríguez, E.; Garrido, R.

    2008-06-01

    Mode identification is one of the first and main problems we encounter in trying to develop the complete potential of asteroseismology. In the particular case of {g}-mode pulsators, this is still an unsolved problem, from both the observational and theoretical points of view. Nevertheless, in recent years, some observational and theoretical efforts have been made to find a solution. In this work we use the latest theoretical and computational tools to understand asymptotic {g}-mode pulsators: 1) the Frequency Ratio Method, and 2) Time Dependent Convection. With these tools, a self-consistent procedure for mode identification and modelling of these {g}-mode pulsators can be constructed. This procedure is illustrated using observational information available for the γ Doradus star 9 Aurigae.

  6. Semi device independence of the BB84 protocol

    NASA Astrophysics Data System (ADS)

    Woodhead, Erik

    2016-05-01

    The BB84 quantum key distribution protocol is semi device independent in the sense that it can be shown to be secure if just one of the users’ devices is restricted to a qubit Hilbert space. Here, we derive an analytic lower bound on the asymptotic secret key rate for the entanglement-based version of BB84 assuming only that one of the users performs unknown qubit POVMs. The result holds against the class of collective attacks and reduces to the well known Shor-Preskill key rate for correlations corresponding to the ideal BB84 correlations mixed with any amount of random noise.

  7. A multi-wavelength study of 30 Doradus: The Interstellar Medium in a low-metallicity galaxy

    NASA Technical Reports Server (NTRS)

    Poglitsch, Albrecht; Genzel, R.; Herrmann, F.; Krabbe, A.; Madden, S. C.; Geis, Norbert; Stacey, G. J.; Townes, C. H.; Johannson, L. E. B.

    1995-01-01

    We report maps of the 158 micron (C II) line, the 63 micron and 146 micron (C I) lines, the 2.2 micron Br gamma line, and the 2.6 mm CO (1-0) line toward the 30 Doradus complex in the Large Magellanic Cloud. The maps of all tracers emphasize the shell-like structure of the 30 Doradus region which is seen edge-on. The fact that the molecular gas as traced by CO (1-0) and the photo dissociated gas as traced by (C II) are co-extensive over tens of parsecs can only be explained by a highly fragmented structure of the interstellar medium which allows UV radiation to penetrate deep into the molecular cloud. Clumpiness is also the key to understanding the extremely high (C II)/CO line intensity ratios.

  8. The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana

    PubMed Central

    Ying, Sheng-Hua; Liu, Jing; Chu, Xin-Ling; Xie, Xue-Qin; Feng, Ming-Guang

    2016-01-01

    Autophagy-related proteins play significantly different roles in eukaryotes. In the entomopathogenic fungus Beauveria bassiana, autophagy is associated with fungal growth and development. BbATG1 (a serine/threonine protein kinase) and BbATG8 (a ubiquitin-like protein) have similar roles in autophagy, but different roles in other processes. Disruption mutants of BbATG1 and BbATG8 had impaired conidial germination under starvation stress. The mutant ΔBbATG8 exhibited enhanced sensitivity to oxidative stress, while a ΔBbATG1 mutant did not. BbATG1 and BbATG8 showed different roles in spore differentiation. The blastospore yield was reduced by 70% and 92% in ΔBbATG1 and ΔBbATG8 mutants, respectively, and the double mutant had a reduction of 95%. Conidial yield was reduced by approximately 90% and 50% in ΔBbATG1 and ΔBbATG8 mutants, respectively. A double mutant had a reduction similar to ΔBbATG1. Additionally, both BbATG1 and BbATG8 affected the levels of conidial protein BbCP15p required for conidiation. The virulence of each autophagy-deficient mutant was considerably weakened as indicated in topical and intrahemocoel injection assays, and showed a greater reduction in topical infection. However, BbATG1 and BbATG8 had different effects on fungal virulence. Our data indicate that these autophagy-related proteins have different functions in fungal stress response, asexual development and virulence. PMID:27197558

  9. The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana.

    PubMed

    Ying, Sheng-Hua; Liu, Jing; Chu, Xin-Ling; Xie, Xue-Qin; Feng, Ming-Guang

    2016-05-20

    Autophagy-related proteins play significantly different roles in eukaryotes. In the entomopathogenic fungus Beauveria bassiana, autophagy is associated with fungal growth and development. BbATG1 (a serine/threonine protein kinase) and BbATG8 (a ubiquitin-like protein) have similar roles in autophagy, but different roles in other processes. Disruption mutants of BbATG1 and BbATG8 had impaired conidial germination under starvation stress. The mutant ΔBbATG8 exhibited enhanced sensitivity to oxidative stress, while a ΔBbATG1 mutant did not. BbATG1 and BbATG8 showed different roles in spore differentiation. The blastospore yield was reduced by 70% and 92% in ΔBbATG1 and ΔBbATG8 mutants, respectively, and the double mutant had a reduction of 95%. Conidial yield was reduced by approximately 90% and 50% in ΔBbATG1 and ΔBbATG8 mutants, respectively. A double mutant had a reduction similar to ΔBbATG1. Additionally, both BbATG1 and BbATG8 affected the levels of conidial protein BbCP15p required for conidiation. The virulence of each autophagy-deficient mutant was considerably weakened as indicated in topical and intrahemocoel injection assays, and showed a greater reduction in topical infection. However, BbATG1 and BbATG8 had different effects on fungal virulence. Our data indicate that these autophagy-related proteins have different functions in fungal stress response, asexual development and virulence.

  10. Role of platelet-derived growth factor-BB (PDGF-BB) in human pulmonary artery smooth muscle cell proliferation.

    PubMed

    Zhao, Yan; Lv, Wentao; Piao, Hongying; Chu, Xiaojie; Wang, Hao

    2014-08-01

    Pulmonary arterial hypertension (PAH) is a vascular remodeling disease characterized by enhanced proliferation of pulmonary artery smooth muscle cells (PASMCs) and suppressed apoptosis. Platelet-derived growth factor (PDGF) is a potent mitogen involved in cell proliferation and migration. PDGF-BB induces the proliferation and migration of PASMCs and has been proposed to be a key mediator in the progression of PAH. Previous studies have shown that PDGF and its receptor are substantially elevated in lung tissues and PASMCs isolated from patients and animals with PAH, but the underlying mechanisms are still poorly manifested. MAP kinases, including extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun NH2-terminal kinase1/2 (JNK1/2), and p38 are the key intracellular signals for stimuli-induced cell proliferation, survival, and apoptosis. Therefore, the purpose of this study is to determine whether PDGF-BB on cell proliferation process is mediated through the MAP kinases pathway in human PASMCs (HPASMCs). Our results showed PDGF-BB-induced proliferating cell nuclear antigen (PCNA), Cyclin A and Cyclin E expression in a concentration-dependent manner. The expression levels of phosphorylated JNK (p-JNK) was upregulated with 20 ng/ml PDGF-BB treatment, while PDGF-BB could not increase phosphorylated ERK1/2 (p-ERK1/2) and p-38 (p-p38) expression. The effects of PDGF-BB on cell proliferation and survival were weakened after the administration of antagonist of the JNK pathway or si-JNK. In addition, PDGF-BB protected against the loss of mitochondrial membrane potentials evoked by serum deprivation (SD) in a JNK-dependent manner. These results suggest that PDGF-BB promotes HPASMCs proliferation and survival, which is likely to be mediated via the JNK pathway.

  11. An Optical and X-Ray Examination of Two Radio Supernova Remnant Candidates in 30 Doradus

    NASA Astrophysics Data System (ADS)

    Chu, You-Hua; Gruendl, Robert A.; Chen, C.-H. Rosie; Lazendic, Jasmina S.; Dickel, John R.

    2004-11-01

    The giant H II region 30 Doradus is known for its violent internal motions and bright diffuse X-ray emission, suggesting the existence of supernova remnants (SNRs), but no nonthermal radio emission has been detected. Recently, Lazendic et al. compared the Hα/Hβ and radio/Hα ratios and suggested two small radio sources to be nonthermal and thus SNR candidates; however, no optical or X-ray counterparts were detected. We have used high-resolution optical images and high-dispersion spectra to examine the morphological, spectral, and kinematic properties of these two SNR candidates and still find no optical evidence supporting their identification as SNRs. We have also determined the X-ray luminosities of these SNR candidates and find them 1-3 orders of magnitude lower than those commonly seen in young SNRs. High extinction can obscure optical and X-ray signatures of an SNR, but would prohibit the use of a high radio/Hα ratio to identify nonthermal radio emission. We suggest that the SNR candidate MCRX J053831.8-690620 is associated with a young star-forming region; while the radio emission originates from the obscured star-forming region, the observed optical emission is dominated by the foreground. We suggest that the SNR candidate MCRX J053838.8-690730 is associated with a dust/molecular cloud, which obscures some optical emission but not the radio emission.

  12. V421 Pegasi: a detached eclipsing binary with a possible γ Doradus component

    NASA Astrophysics Data System (ADS)

    Özdarcan, O.; Çakırlı, Ö.; Akan, C.

    2016-07-01

    We present spectroscopic and photometric study of V421 Peg. This eclipsing binary displays lines from both components that are well separated. This allowed us to classify the primary and secondary component as F(1 ± 0.5) V and F(2 ± 0.5) V, respectively. We use our radial velocity measurements together with Hipparcos and ASAS photometry and apply simultaneous analysis, which yields masses and radii of the primary and secondary components as M1 = 1.594 ± 0.029 M⊙, M2 = 1.356 ± 0.029 M⊙ and R1 = 1.584 ± 0.028 R⊙, R2 = 1.328 ± 0.029 R⊙, respectively. Positions of the components in HR diagram suggest that the primary component is a γ Doradus variable candidate. Spectroscopic and photometric properties of the system indicates reddening value of E(B - V) = 0m.021 which puts the system to the distance of 158 ± 4 pc.

  13. LIKELY MEMBERS OF THE {beta} PICTORIS AND AB DORADUS MOVING GROUPS IN THE NORTH

    SciTech Connect

    Schlieder, Joshua E.; Lepine, Sebastien; Simon, Michal E-mail: michal.simon@stonybrook.edu

    2012-10-01

    We present first results from follow-up of targets in the northern hemisphere {beta} Pictoris and AB Doradus moving group candidate list of Schlieder et al. We obtained high-resolution, near-infrared spectra of 27 candidate members to measure their radial velocities and confirm consistent group kinematics. We identify 15 candidates with consistent predicted and measured radial velocities, perform analyses of their six-dimensional (UVWXYZ) Galactic kinematics, and compare to known group member distributions. Based on these analyses, we propose that seven {beta} Pic and eight AB Dor candidates are likely new group members. Four of the likely new {beta} Pic stars are binaries, one a double-lined spectroscopic system. Three of the proposed AB Dor stars are binaries. Counting all binary components, we propose 22 likely members of these young, moving groups. The majority of the proposed members are M2 to M5 dwarfs, the earliest being of type K2. We also present preliminary parameters for the two new spectroscopic binaries identified in the data, the proposed {beta} Pic member and a rejected {beta} Pic candidate. Our candidate selection and follow-up has thus far identified more than 40 low-mass, likely members of these two moving groups. These stars provide a new sample of nearby, young targets for studies of local star formation, disks and exoplanets via direct imaging, and astrophysics in the low-mass regime.

  14. COOL YOUNG STARS IN THE NORTHERN HEMISPHERE: {beta} PICTORIS AND AB DORADUS MOVING GROUP CANDIDATES

    SciTech Connect

    Schlieder, Joshua E.; Simon, Michal; Lepine, Sebastien E-mail: schlieder@mpia-hd.mpg.de

    2012-04-15

    As part of our continuing effort to identify new, low-mass members of nearby, young moving groups (NYMGs), we present a list of young, low-mass candidates in the northern hemisphere. We used our proven proper-motion selection procedure and ROSAT X-ray and GALEX-UV activity indicators to identify 204 young stars as candidate members of the {beta} Pictoris and AB Doradus NYMGs. Definitive membership assignment of a given candidate will require a measurement of its radial velocity and distance. We present a simple system of indices to characterize the young candidates and help prioritize follow-up observations. New group members identified in this candidate list will be high priority targets for (1) exoplanet direct imaging searches, (2) the study of post-T-Tauri astrophysics, (3) understanding recent local star formation, and (4) the study of local galactic kinematics. Information available now allows us to identify eight likely new members in the list. Two of these, a late-K and an early-M dwarf, we find to be likely members of the {beta} Pic group. The other six stars are likely members of the AB Dor moving group. These include an M dwarf triple system, and three very cool objects that may be young brown dwarfs, making them the lowest-mass, isolated objects proposed in the AB Dor moving group to date.

  15. Characterizing the AB Doradus moving group via high-resolution spectroscopy and kinematic traceback

    SciTech Connect

    McCarthy, Kyle; Wilhelm, Ronald J.

    2014-10-01

    We present a detailed analysis of 10 proposed F and G members of the nearby, young moving group AB Doradus (ABD). Our sample was obtained using the 2.7 m telescope at the McDonald Observatory with the coude echelle spectrograph, achieving R ∼ 60,000 and signal-to-noise ratio ∼200. We derive spectroscopic T {sub eff}, log(g), [Fe/H], and microturbulance (v{sub t} ) using a bootstrap method of the TGVIT software resulting in typical errors of 33K in T {sub eff}, 0.08 dex in log(g), 0.03 dex in [Fe/H], and 0.13 km s{sup –1} in v{sub t} . Characterization of the ABD sample is performed in three ways: (1) chemical homogeneity, (2) kinematic traceback, and (3) isochrone fitting. We find the average metal abundance is [M/H] = –0.03 ± 0.06 with a traceback age of 125 Myr. Our stars were fit to three different evolutionary models and we found that the best match to our ABD sample is the YREC [M/H] = –0.1 model. In our sample of 10 stars, we identify 1 star that is a probable non-member, 3 enigmatic stars, and 6 stars with confirmed membership. We also present a list of chemically coherent stars from this study and the Barenfeld et al. study.

  16. A GRAND VIEW OF THE BIRTH OF 'HEFTY' STARS - 30 DORADUS NEBULA MONTAGE

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This picture, taken in visible light with the Hubble Space Telescope's Wide Field and Planetary Camera 2 (WFPC2), represents a sweeping view of the 30 Doradus Nebula. But Hubble's infrared camera - the Near Infrared Camera and Multi-Object Spectrometer (NICMOS) - has probed deeper into smaller regions of this nebula to unveil the stormy birth of massive stars. The montages of images in the upper left and upper right represent this deeper view. Each square in the montages is 15.5 light-years (19 arcseconds) across. The brilliant cluster R136, containing dozens of very massive stars, is at the center of this image. The infrared and visible-light views reveal several dust pillars that point toward R136, some with bright stars at their tips. One of them, at left in the visible-light image, resembles a fist with an extended index finger pointing directly at R136. The energetic radiation and high-speed material emitted by the massive stars in R136 are responsible for shaping the pillars and causing the heads of some of them to collapse, forming new stars. The infrared montage at upper left is enlarged in an accompanying image. Credits for NICMOS montages: NASA/Nolan Walborn (Space Telescope Science Institute, Baltimore, Md.) and Rodolfo Barba' (La Plata Observatory, La Plata, Argentina) Credits for WFPC2 image: NASA/John Trauger (Jet Propulsion Laboratory, Pasadena, Calif.) and James Westphal (California Institute of Technology, Pasadena, Calif.)

  17. From gas to stars in energetic environments: dense gas clumps in the 30 Doradus region within the Large Magellanic Cloud

    SciTech Connect

    Anderson, Crystal N.; Meier, David S.; Ott, Jürgen; Hughes, Annie; Wong, Tony; Looney, Leslie; Henkel, Christian; Chen, Rosie; Indebetouw, Remy; Muller, Erik; Pineda, Jorge L.; Seale, Jonathan

    2014-09-20

    We present parsec-scale interferometric maps of HCN(1-0) and HCO{sup +}(1-0) emission from dense gas in the star-forming region 30 Doradus, obtained using the Australia Telescope Compact Array. This extreme star-forming region, located in the Large Magellanic Cloud (LMC), is characterized by a very intense ultraviolet ionizing radiation field and sub-solar metallicity, both of which are expected to impact molecular cloud structure. We detect 13 bright, dense clumps within the 30 Doradus-10 giant molecular cloud. Some of the clumps are aligned along a filamentary structure with a characteristic spacing that is consistent with formation via varicose fluid instability. Our analysis shows that the filament is gravitationally unstable and collapsing to form stars. There is a good correlation between HCO{sup +} emission in the filament and signatures of recent star formation activity including H{sub 2}O masers and young stellar objects (YSOs). YSOs seem to continue along the same direction of the filament toward the massive compact star cluster R136 in the southwest. We present detailed comparisons of clump properties (masses, linewidths, and sizes) in 30Dor-10 to those in other star forming regions of the LMC (N159, N113, N105, and N44). Our analysis shows that the 30Dor-10 clumps have similar masses but wider linewidths and similar HCN/HCO{sup +} (1-0) line ratios as clumps detected in other LMC star-forming regions. Our results suggest that the dense molecular gas clumps in the interior of 30Dor-10 are well shielded against the intense ionizing field that is present in the 30 Doradus region.

  18. Detecting non-uniform period spacings in the Kepler photometry of γ Doradus stars: methodology and case studies

    NASA Astrophysics Data System (ADS)

    Van Reeth, T.; Tkachenko, A.; Aerts, C.; Pápics, P. I.; Degroote, P.; Debosscher, J.; Zwintz, K.; Bloemen, S.; De Smedt, K.; Hrudkova, M.; Raskin, G.; Van Winckel, H.

    2015-02-01

    Context. The analysis of stellar oscillations is one of the most reliable ways to probe stellar interiors. Recent space missions such as Kepler have provided us with an opportunity to study these oscillations with unprecedented detail. For many multi-periodic pulsators such as γ Doradus stars, this led to the detection of dozens to hundreds of oscillation frequencies that could not be found from ground-based observations. Aims: We aim to detect non-uniform period spacings in the Fourier spectra of a sample of γ Doradus stars observed by Kepler. Such detection is complicated by both the large number of significant frequencies in the space photometry and by overlapping non-equidistant rotationally split multiplets. Methods: Guided by theoretical properties of gravity-mode oscillation of γ Doradus stars, we developed a period-spacing detection method and applied it to Kepler observations of a few stars, after having tested the performance from simulations. Results: The application of the technique resulted in the clear detection of non-uniform period spacing series for three out of the five treated Kepler targets. Disadvantages of the technique are also discussed, and include the disability to distinguish between different values of the spherical degree and azimuthal order of the oscillation modes without additional theoretical modelling. Conclusions: Despite the shortcomings, the method is shown to allow solid detections of period spacings for γ Doradus stars, which will allow future asteroseismic analyses of these stars. Based on data gathered with the NASA Discovery mission Kepler and the HERMES spectrograph, which is installed at the Mercator Telescope, operated on the island of La Palma by the Flemish Community at the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias, and supported by the Fund for Scientific Research of Flanders (FWO), Belgium, the Research Council of KU Leuven, Belgium, the Fonds National de la

  19. Section BB Hatch Coating; Framing Plan on Line C Lodging ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Section B-B Hatch Coating; Framing Plan on Line C Lodging Knees at Hatch; Elevation A-A Hull Framing; Section at Hatch Frame 36, Starboard Looking Aft; Midship Section Frame 37, Port Looking Aft - Steam Schooner WAPAMA, Kaiser Shipyard No. 3 (Shoal Point), Richmond, Contra Costa County, CA

  20. Controlling Works, Section AA at Bear Trap Dam, Section BB ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Controlling Works, Section A-A at Bear Trap Dam, Section B-B at Bear-Trap Dam, Section C-C at Sluice Gate - Chicago Sanitary and Ship Canal, Lockport Controlling Works, Illinois Waterway River Mile 293.2, Lockport, Will County, IL

  1. BB: Half Section; Top of Engine; Valve Gear Detail; CC: ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    B-B: Half Section; Top of Engine; Valve Gear Detail; C-C: Top of Condenser; D-D: Condenser Interior; Air Pump Piston; Air Pump Lever; Water Pump - Steamboat COLUMBUS, Submerged south-southeast of Point Lookout, Scotland, St. Mary's County, MD

  2. 32. SECTIONS AA, BB, CC, DD, AND EE WASTE CALCINATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. SECTIONS A-A, B-B, C-C, D-D, AND E-E WASTE CALCINATION FACILITY SHOWING RELATIONSHIPS OF DIFFERENT FLOOR LEVELS TO ONE ANOTHER. INEEL DRAWING NUMBER 200-0633-00-287-106353. FLUOR NUMBER 5775-CPP-633-A-3. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  3. A milestone toward understanding PDR properties in the extreme environment of LMC-30 Doradus

    NASA Astrophysics Data System (ADS)

    Chevance, M.; Madden, S. C.; Lebouteiller, V.; Godard, B.; Cormier, D.; Galliano, F.; Hony, S.; Indebetouw, R.; Le Bourlot, J.; Lee, M.-Y.; Le Petit, F.; Pellegrini, E.; Roueff, E.; Wu, R.

    2016-05-01

    Context. More complete knowledge of galaxy evolution requires understanding the process of star formation and the interaction between the interstellar radiation field and interstellar medium (ISM) in galactic environments traversing a wide range of physical parameter space. We focus on the impact of massive star formation on the surrounding low metallicity ISM in 30 Doradus in the Large Magellanic Cloud (LMC). A low metal abundance, which can characterizes some galaxies of the early Universe, results in less ultraviolet (UV) shielding for the formation of the molecular gas necessary for star formation to proceed. The half-solar metallicity gas in this region is strongly irradiated by the super star cluster R136, making it an ideal laboratory to study the structure of the ISM in an extreme environment. Aims: Our goal is to construct a comprehensive, self-consistent picture of the density, radiation field, and ISM structure in the most active star-forming region in the LMC, 30 Doradus. Our spatially resolved study investigates the gas heating and cooling mechanisms, particularly in the photodissociation regions (PDR) where the chemistry and thermal balance are regulated by far-UV photons (6 eV < hν < 13.6 eV). Methods: We present Herschel observations of far-infrared (FIR) fine-structure lines obtained with PACS and SPIRE/FTS. We combined atomic fine-structure lines from Herschel and Spitzer observations with ground-based CO data to provide diagnostics on the properties and structure of the gas by modeling it with the Meudon PDR code. For each tracer we estimate the possible contamination from the ionized gas to isolate the PDR component. We derive the spatial distribution of the radiation field, the pressure, the size, and the filling factor of the photodissociated gas and molecular clouds. Results: We find a range of pressure of ~105-1.7 × 106 cm-3 K and a range of incident radiation field GUV~102-2.5 × 104 through PDR modeling. Assuming a plane

  4. On the common origin of the AB Doradus moving group and the Pleiades cluster

    NASA Astrophysics Data System (ADS)

    Ortega, V. G.; Jilinski, E.; de La Reza, R.; Bazzanella, B.

    2007-05-01

    AB Doradus (AB Dor) is the nearest identified moving group. As with other such groups, the age is important for understanding of several key questions. It is important, for example, in establishing the origin of the group and also in comparative studies of the properties of planetary systems, eventually surrounding some of the AB Dor group members, with those existing in other groups. For AB Dor two rather different estimates for its age have been proposed: the first one, of the order of 50 Myr, by Zuckerman and coworkers from a comparison with the Tucana/Horologium moving group and a second one of about 100-125 Myr by Luhman and coworkers from colour-magnitude diagrams. Using this last value and the closeness in velocity space of AB Dor and the Pleiades galactic cluster, Luhman and coworkers suggested coevality for these systems. Because strictly speaking such a closeness does not still guarantee coevality, here we address this problem by computing and comparing the full 3D orbits of AB Dor, Pleiades, α Persei and IC 2602. The latter two open clusters have estimated ages of about 85-90 and 50 Myr. The resulting age 119 +/- 20 Myr is consistent with AB Dor and Pleiades being coeval. Our solution and the scenario of open cluster formation proposed by Kroupa and collaborators suggest that the AB Dor moving group may be identified with the expanding subpopulation (Group I) present in this scenario. We also discuss other related aspects as iron and lithium abundances, eventual stellar mass segregation during the formation of the systems and possible fraction of debris discs in the AB Dor group.

  5. {beta} PICTORIS AND AB DORADUS MOVING GROUPS: LIKELY NEW LOW-MASS MEMBERS

    SciTech Connect

    Schlieder, Joshua E.; Simon, Michal; Lepine, Sebastien E-mail: michal.simon@stonybrook.ed

    2010-07-15

    We present results from our continuing program to identify new, low-mass, members of the nearby young moving groups (NYMGs) using a proper motion selection algorithm and various observational techniques. We have three goals: (1) to provide high priority targets for exoplanet searches by direct imaging, (2) to complete the census of the membership in the NYMGs down to {approx}0.1 M{sub sun}, and thus (3) provide a well-characterized sample of nearby (median distances at least twice as close as the Taurus and Ophiuchus star-forming regions), young (8-50 Myr) stars for detailed study of their physical properties and multiplicity. Our program proceeds as follows: we apply the selection algorithm to a proper motion catalog where initial selection cuts of candidate members are based on the mean motion of known NYMG members and the proper motions and photometric distances of the candidates. NYMG membership is investigated further using possible signs of youth, including H{alpha} emission and X-ray flux, and then verified through radial velocity measurements. We identify TYC 1766-1431-1 (M3), TYC 1208-468-1 and 2 (K3), TYC 7558-655-1 (K5), and PM I04439+3723W and E (M3) as likely members of the {beta} Pictoris moving group (BPMG) and TYC 1741-2117-1N and S (K7), TYC 1752-63-1 (K7), TYC 523-573-1 (K7), and TYC 4943-192-1 (M0) as likely members of the AB Doradus moving group (ABDMG). We also rule out the membership of several BPMG and ABDMG candidates. To date our program has identified 16 new NYMG members of spectral type K3 or later.

  6. OT2_jpineda_2: Large-scale dynamics and the formation of clouds and stars in the 30 Doradus region of the Large Magellanic Cloud

    NASA Astrophysics Data System (ADS)

    Pineda, J.

    2011-09-01

    Understanding the processes governing the formation of clouds and stars in merging systems is key for the study of how galaxies evolved in the early Universe. The 30 Doradus region in the low-metallicity Large Magellanic Cloud (LMC) is the nearest example of this process, resulting from the interaction between the LMC and the halo of the Milky Way. This makes 30 Doradus the prime laboratory to study these large-scale dynamical processes under conditions that are similar to those at early cosmological times. We propose to use Herschel to obtain a large-scale uniform sampling of this region in [CII] 158um, [NII] 122um and 205um, and [OI] 63um and 146um lines with PACS, and at selected positions in [CII] with HIFI. With this data we will derive the large-scale distribution of the density and pressure of the low-metallicity gas revealing the characteristic signatures of shocked gas. This will then be used to determine the relationships among the diffuse, "dark H2", and dense molecular gas in the 30 Doradus region. We will also derive the electron density distribution of the gas and from this the contribution from ionized gas to the observed [CII] emission. The proposed observations will allow us to study the effect of large-scale gas compression in the multiphase, low-metallicity interstellar medium of 30 Doradus. This information will be valuable for the interpretation of future observations of [CII] in high-redshift galaxies made with ALMA

  7. Hubble Tarantula Treasury Project. II. The Star-formation History of the Starburst Region NGC 2070 in 30 Doradus

    NASA Astrophysics Data System (ADS)

    Cignoni, M.; Sabbi, E.; van der Marel, R. P.; Tosi, M.; Zaritsky, D.; Anderson, J.; Lennon, D. J.; Aloisi, A.; de Marchi, G.; Gouliermis, D. A.; Grebel, E. K.; Smith, L. J.; Zeidler, P.

    2015-10-01

    We present a study of the recent star formation (SF) of 30 Doradus in the Large Magellanic Cloud (LMC) using the panchromatic imaging survey Hubble Tarantula Treasury Project. In this paper we focus on the stars within 20 pc of the center of 30 Doradus, the starburst region NGC 2070. We recovered the SF history by comparing deep optical and near-infrared color-magnitude diagrams (CMDs) with state-of-the-art synthetic CMDs generated with the latest PAdova and TRieste Stellar Evolution Code (PARSEC) models, which include all stellar phases from pre-main-sequence to post-main-sequence. For the first time in this region we are able to measure the SF using intermediate- and low-mass stars simultaneously. Our results suggest that NGC 2070 experienced prolonged activity. In particular, we find that the SF in the region (1) exceeded the average LMC rate ≈ 20 Myr ago, (2) accelerated dramatically ≈ 7 Myr ago, and (3) reached a peak value 1-3 Myr ago. We did not find significant deviations from a Kroupa initial mass function down to 0.5 {M}⊙ . The average internal reddening E(B-V) is found to be between 0.3 and 0.4 mag. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA Inc., under NASA contract NAS 5-26555.

  8. Randomness determines practical security of BB84 quantum key distribution

    NASA Astrophysics Data System (ADS)

    Li, Hong-Wei; Yin, Zhen-Qiang; Wang, Shuang; Qian, Yong-Jun; Chen, Wei; Guo, Guang-Can; Han, Zheng-Fu

    2015-11-01

    Unconditional security of the BB84 quantum key distribution protocol has been proved by exploiting the fundamental laws of quantum mechanics, but the practical quantum key distribution system maybe hacked by considering the imperfect state preparation and measurement respectively. Until now, different attacking schemes have been proposed by utilizing imperfect devices, but the general security analysis model against all of the practical attacking schemes has not been proposed. Here, we demonstrate that the general practical attacking schemes can be divided into the Trojan horse attack, strong randomness attack and weak randomness attack respectively. We prove security of BB84 protocol under randomness attacking models, and these results can be applied to guarantee the security of the practical quantum key distribution system.

  9. Randomness determines practical security of BB84 quantum key distribution.

    PubMed

    Li, Hong-Wei; Yin, Zhen-Qiang; Wang, Shuang; Qian, Yong-Jun; Chen, Wei; Guo, Guang-Can; Han, Zheng-Fu

    2015-11-10

    Unconditional security of the BB84 quantum key distribution protocol has been proved by exploiting the fundamental laws of quantum mechanics, but the practical quantum key distribution system maybe hacked by considering the imperfect state preparation and measurement respectively. Until now, different attacking schemes have been proposed by utilizing imperfect devices, but the general security analysis model against all of the practical attacking schemes has not been proposed. Here, we demonstrate that the general practical attacking schemes can be divided into the Trojan horse attack, strong randomness attack and weak randomness attack respectively. We prove security of BB84 protocol under randomness attacking models, and these results can be applied to guarantee the security of the practical quantum key distribution system.

  10. Randomness determines practical security of BB84 quantum key distribution

    PubMed Central

    Li, Hong-Wei; Yin, Zhen-Qiang; Wang, Shuang; Qian, Yong-Jun; Chen, Wei; Guo, Guang-Can; Han, Zheng-Fu

    2015-01-01

    Unconditional security of the BB84 quantum key distribution protocol has been proved by exploiting the fundamental laws of quantum mechanics, but the practical quantum key distribution system maybe hacked by considering the imperfect state preparation and measurement respectively. Until now, different attacking schemes have been proposed by utilizing imperfect devices, but the general security analysis model against all of the practical attacking schemes has not been proposed. Here, we demonstrate that the general practical attacking schemes can be divided into the Trojan horse attack, strong randomness attack and weak randomness attack respectively. We prove security of BB84 protocol under randomness attacking models, and these results can be applied to guarantee the security of the practical quantum key distribution system. PMID:26552359

  11. Silver halide sensitized gelatin derived from BB-640 holographic emulsion.

    PubMed

    Neipp, C; Pascual, I; Beléndez, A

    1999-03-10

    Silver halide sensitized gelatin (SHSG) is one of the most interesting techniques for the production of holographic optical elements, achieving relatively high sensitivity of photographic material with a low scattering of dichromated gelatin. Here we present experimental results for SHSG derived from the novel BB-640, a red-sensitive ultra-fine-grain emulsion from Holographic Recording Technologies (Steinau, Germany). The material is characterized before recording and after processing, and information about the thickness, absorption, and refractive-index modulation of the final holograms is obtained. The influence of the developer is analyzed, and diffraction efficiencies as great as 96.2% (after allowing for reflections) with a transmission of 1% and absorption and scatter losses of 2.8% are obtained with AAC developer. Our investigations reveal that high-quality SHSG transmission holograms may be obtained with the new BB-640 plates.

  12. Biotreatment of chromite ore processing residue by Pannonibacter phragmitetus BB.

    PubMed

    Wang, Yangyang; Yang, Zhihui; Peng, Bing; Chai, Liyuan; Wu, Baolin; Wu, Ruiping

    2013-08-01

    Chromite ore processing residues (COPR) is the source of the Cr(VI) contamination in the environment. Pannonibacter phragmitetus BB was used to treat two different types of COPRs in this research. The water-soluble Cr(VI) of COPR A and B is 3,982.9 and 1,181.4 mg/kg, respectively. In the column biotreatment process, P. phragmitetus BB can reduce Cr(VI) in the leachate to an undetectable level at the flow rate of 1 and 2 ml/min. In the direct biotreatment process, Cr(VI) in the liquid supernatant of COPR A and B decreased from 265 and 200 mg/l to 145 and 40 mg/kg after 240 h of incubation. In one-step and two-step biotreatment processes, Cr(VI) in the liquid supernatant of both COPRs can be reduced to an undetectable level. The toxicity characteristic leaching procedure results indicate that the Cr(VI) concentration of treated COPR A (3.48 mg/l) is lower than the identification standards for hazardous wastes of China (5 mg/l) (GB 5085.6-2007). The information obtained in this study has significance for the application of P. phragmitetus BB to remediate COPR contamination.

  13. A GRAND VIEW OF THE BIRTH OF 'HEFTY' STARS - 30 DORADUS NEBULA DETAILS

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These are two views of a highly active region of star birth located northeast of the central cluster, R136, in 30 Doradus. The orientation and scale are identical for both views. The top panel is a composite of images in two colors taken with the Hubble Space Telescope's visible-light camera, the Wide Field and Planetary Camera 2 (WFPC2). The bottom panel is a composite of pictures taken through three infrared filters with Hubble's Near Infrared Camera and Multi-Object Spectrometer (NICMOS). In both cases the colors of the displays were chosen to correlate with the nebula's and stars' true colors. Seven very young objects are identified with numbered arrows in the infrared image. Number 1 is a newborn, compact cluster dominated by a triple system of 'hefty' stars. It has formed within the head of a massive dust pillar pointing toward R136. The energetic outflows from R136 have shaped the pillar and triggered the collapse of clouds within its summit to form the new stars. The radiation and outflows from these new stars have in turn blown off the top of the pillar, so they can be seen in the visible-light as well as the infrared image. Numbers 2 and 3 also pinpoint newborn stars or stellar systems inside an adjacent, bright-rimmed pillar, likewise oriented toward R136. These objects are still immersed within their natal dust and can be seen only as very faint, red points in the visible-light image. They are, however, among the brightest objects in the infrared image, since dust does not block infrared light as much as visible light. Thus, numbers 2 and 3 and number 1 correspond respectively to two successive stages in the birth of massive stars. Number 4 is a very red star that has just formed within one of several very compact dust clouds nearby. Number 5 is another very young triple-star system with a surrounding cluster of fainter stars. They also can be seen in the visible-light picture. Most remarkable are the glowing patches numbered 6 and 7, which astronomers

  14. Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink.

    PubMed

    Babavalian, H; Latifi, A M; Shokrgozar, M A; Bonakdar, S; Tebyanian, H; Shakeri, F

    2016-07-31

    The PDGF-BB plays a key role in several pathogenesis diseases and it is believed to be an important mediator for wound healing. The recombinant human PDGF-BB is safe and effective to stimulate the healing of chronic, full thickness and lower extremity diabetic neurotrophic ulcers. In the present study, we attempted to produce a PDGF-BB growth factor and also, evaluate its functionality in cell proliferation in yeast host Pichia pink. Pichia pink yeast was used as a host for evaluation of the rhPDGF-BB expression. The coding sequence of PDGF-BB protein was synthesized after optimization and packed into the pGEM. Recombinant proteins were produced and purified. The construct of pPinkα-HC-pdgf was confirmed by sequence, the PDGF-BB protein was expressed and purified with using a nickel affinity chromatography column and then characterized by SDS-PAGE electrophoresis. The biological activity of PDGF-BB was estimated with using human fibroblast cell line. The measurement of protein concentration was determined by Bradford and human PDGF-BB ELISA kit. Purified rhPDGF-BB showed similar biological activity (as the standard PDGF-BB) and suggested that the recombinant protein has a successful protein expression (as well as considerable biological activity in P. pink host). The exact amount of recombinant PDGF-BB concentrations were measured by specific ELISA test which it was about 30 μg/ml. Our study suggested that efficiency of biological activity of PDGF-BB protein may be related to its conformational similarity with standard type and also, it practically may be important in wound healing and tissue regeneration.

  15. Role of endogenous PDGF-BB in cultured cardiomyocytes exposed to hypoxia.

    PubMed

    Rong, Rong; Wang, You-Cui; Hu, Li-qun; He, Qin-qin; Zhou, Xin-Fu; Wang, Ting-hua; Bu, Pei-li

    2015-04-01

    Platelet-derived growth factor-BB (PDGF-BB) plays a critical role in cell proliferation, angiogenesis and fibrosis. However, its exact role in cardiomyocytes exposed to hypoxia is not well known. This study was therefore designed to detect whether PDGF-BB expression was changed in a hypoxic condition, then the possible role of endogenous PDGF-BB in cardiomyocytes was explored, with interference RNA in a lentiviral vector ex vivo. The results showed that cultured cardiomyocytes exhibited an optimal proliferation from 3 to 10 days. However, LDH level was significantly increased but the heart rhythm was not altered in cardiomyocytes exposed to hypoxia for 24 hours. PDGF-BB expression was substantially upregulated in hypoxic cardiomyocytes. In order to know the role of PDGF-BB, we performed PDGF-BB knockdown in cultured cardiomyocytes. The number of apoptotic cells and the level of LDH were significantly increased but the beat rhythm was reduced in cardiomyocytes with PDGF-BB knockdown. These findings suggest that endogenous PDGF-BB exerts a crucial protective effect to cultured cardiomyocytes exposed to hypoxia.

  16. The CoRoT star ID 100866999: a hybrid γ Doradus-δ Scuti star in an eclipsing binary system

    NASA Astrophysics Data System (ADS)

    Chapellier, E.; Mathias, P.

    2013-08-01

    Context. The presence of g- and p-modes allows testing stellar models from the core to the envelope. Moreover, binarity in an eclipsing system constrains the physical parameters of the pulsating star. Aims: CoRot ID 100866999 is a relatively large-amplitude hybrid γ Doradus-δ Scuti star with two clearly distinct frequency domains. The large number of detected frequencies allows a detailed study of the interaction between them. In addition, we can derive the fundamental parameters of both components from the study of the eclipsing light curve. Methods: After removing the eclipsing phases, we analyzed the data with the Period04 package up to a signal-to-noise ratio S/N = 4. The light curve was then prewhitened with these oscillation frequencies to derive the fundamental parameters of the two components. Results: The eclipsing light curve analysis results in a (1.8+1.1) M⊙ system, both components being main sequence stars. We detect 124 frequencies related to luminosity variations of the primary. They are present in two well-separated domains: 89 frequencies in the interval [0.30;3.64] d-1 and 35 in the interval [14.57; 33.96] d-1. There are 22 γ Doradus frequencies separated by a constant period interval ΔP = 0.03493 d. These frequencies correspond to a series of g-modes of degree ℓ = 1 with successive radial orders k. We identify 21 linear combinations between the first nine γ Doradus frequencies. The δ Scuti domain is dominated by a large-amplitude frequency F = 16.9803 d-1. The eight first γ Doradus frequencies fi are present with much lower amplitude in the δ Scuti domain as F ± fi. These interactions between g- and p-modes confirm the phenomenon we detected in another CoRoT star. The amplitude and the phase of the main frequency F shows a double-wave modulation along the orbital phase, giving rise to series of combination frequencies. Such combination frequencies are also detected, with lower amplitude, for the first γ Doradus modes. The Co

  17. Silver-halide sensitized gelatin holograms from BB-640 plates

    NASA Astrophysics Data System (ADS)

    Belendez, Augusto; Neipp, Cristian; Pascual, Inmaculada V.

    1999-03-01

    In this paper we present the results of our research on the recording of SHSG holographic transmission gratings using the new BB-640 photographic emulsion, a red sensitive ultra-fine- grained emulsion from Holographic Recording Technologies. The exposed plates were processed following a process similar to that optimized by Fimia et al. for Agfa 8E75 HD plates but with the temperature of the bleaching bath modified to 70 degrees Celsius. This increase in temperature improves the diffraction efficiency but does not produce an increase in absorption and scattering, as opposed to what happens in the case of the Agfa 8E75 HD emulsion. The influence of the developer on various holographic parameters is analyzed and discussed.

  18. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... 63.7(f) Alternative Test Method Yes. 63.7(g) Data Analysis Yes. 63.7(h) Waiver of Tests Yes. 63.8(a... Method Yes. 63.8(f)(6) Alternative to RATA Test No Subpart BB does not require CEMS. 63.8(g)(1) Data.... A CMS performance report is not required. 63.10(e)(4) COMS Data Reports No Subpart BB does...

  19. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... 63.7(f) Alternative Test Method Yes. 63.7(g) Data Analysis Yes. 63.7(h) Waiver of Tests Yes. 63.8(a... Method Yes. 63.8(f)(6) Alternative to RATA Test No Subpart BB does not require CEMS. 63.8(g)(1) Data.... A CMS performance report is not required. 63.10(e)(4) COMS Data Reports No Subpart BB does...

  20. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... 63.7(f) Alternative Test Method Yes. 63.7(g) Data Analysis Yes. 63.7(h) Waiver of Tests Yes. 63.8(a... Method Yes. 63.8(f)(6) Alternative to RATA Test No Subpart BB does not require CEMS. 63.8(g)(1) Data.... A CMS performance report is not required. 63.10(e)(4) COMS Data Reports No Subpart BB does...

  1. Platelet-derived growth factor-BB-mediated glycosaminoglycan synthesis is transduced through Akt.

    PubMed

    Cartel, Nicholas J; Wang, Jinxia; Post, Martin

    2002-04-01

    Previously we have demonstrated that the phosphoinositide 3-kinase (PI-3K) signal-transduction pathway mediates platelet-derived growth factor (PDGF)-BB-induced glycosaminoglycan (GAG) synthesis in fetal lung fibroblasts. In the present study we further investigated the signal-transduction pathway(s) that results in PDGF-BB-induced GAG synthesis. Over-expression of a soluble PDGF beta-receptor as well as a mutated form of the beta-receptor, unable to bind PI-3K, diminished GAG synthesis in fetal lung fibroblasts subsequent to PDGF-BB stimulation. The PI-3K inhibitor wortmannin blocked PDGF-BB-induced Akt activity as well as significantly diminishing PDGF-BB-mediated GAG synthesis. Expression of dominant-negative PI-3K also abrogated Akt activity and GAG synthesis. Furthermore, expression of dominant-negative Akt abrogated endogenous Akt activity, Rab3D phosphorylation and GAG synthesis, whereas expression of constitutively activated Akt stimulated Rab3D phosphorylation and GAG synthesis in the absence of PDGF-BB. Over-expression of wild-type PTEN (phosphatase and tensin homologue deleted in chromosome 10) inhibited Akt activity and concomitantly attenuated GAG synthesis in fibroblasts stimulated with PDGF-BB. These data suggest that Akt is an integral protein involved in PDGF-BB-mediated GAG regulation in fetal lung fibroblasts.

  2. 76 FR 13615 - B&B Manufacturing Site; Mobile, Mobile County, AL; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... AGENCY B&B Manufacturing Site; Mobile, Mobile County, AL; Notice of Settlement AGENCY: Environmental... Manufacturing Site located in Mobile, Mobile County, Alabama for publication. DATES: The Agency will consider... No. EPA-RO4- SFUND-2011-0192 or Site name B&B Manufacturing Superfund Site by one of the...

  3. Controlled delivery of platelet-derived growth factor-BB from injectable microsphere/hydrogel composites.

    PubMed

    Wu, Hua; Liu, Jiaoyan; Wu, Jingjing; Wan, Ying; Chen, Yun

    2016-12-01

    Platelet-derived growth factor-BB (PGDF-BB) loaded gelatin microspheres with an average size of about 2μm was incorporated into chitosan/silk fibroin/glycerophosphate (GP) solutions to prepare composites. The formulated composite solutions were able to form into hydrogels in a temperature range between 32 and 37°C at a pH of ca.7. They had good fluidity at 25°C and showed shear-thinning features at both 25 and 37°C, revealing that they are injectable at room temperature. Elastic modulus of some composites at 37°C was about 10-fold higher than that of chitosan/GP gel, confirming that these composites behave like mechanically strong gels. Optimal composites showed abilities to administrate PDGF-BB release in an approximately linear manner up to 5 weeks. The PDGF-BB release could be regulated by the PDGF-BB load and the silk fibroin content in the composites in an individual or cooperative way. In vivo degradation of composites demonstrated that some of them had markedly enhanced degradation endurance as compared to the chitosan/GP gel. PDGF-BB-stimulated DNA synthesis in Balb/c 3T3 fibroblasts and PDGF-BB-induced cell migration suggested that the bioactivity of released PDGF-BB was well retained.

  4. Serum creatine kinase isoenzyme BB is a poor index to the size of various brain lesions.

    PubMed

    Schwartz, J G; Bazan, C; Gage, C L; Prihoda, T J; Gillham, S L

    1989-04-01

    We divided patients with brain lesions into three groups: (a) patients with primary or metastatic brain cancer, (b) brain infarctions, and (c) brain contusion(s). We analyzed each patient's sera for creatine kinase isoenzyme BB (CK-BB), using a monoclonal antibody kit (Impres-BB; International Immunoassay Laboratories). Computerized axial tomography (CAT) scans were performed on each patient. The size of the various lesions was measured from the CAT scan and recorded in milliliters. Total CK, CK-BB, and their ratios were compared with the volume of damaged brain tissue. We found no correlation between any of the variables and the various brain lesions. We attribute this lack of correlation to an intact blood-brain barrier, the rapid elimination or inactivation of CK-BB, or some combination of these factors.

  5. Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients.

    PubMed

    Paul, Gesine; Zachrisson, Olof; Varrone, Andrea; Almqvist, Per; Jerling, Markus; Lind, Göran; Rehncrona, Stig; Linderoth, Bengt; Bjartmarz, Hjalmar; Shafer, Lisa L; Coffey, Robert; Svensson, Mikael; Mercer, Katarina Jansson; Forsberg, Anton; Halldin, Christer; Svenningsson, Per; Widner, Håkan; Frisén, Jonas; Pålhagen, Sven; Haegerstrand, Anders

    2015-03-02

    BACKGROUND. Recombinant human PDGF-BB (rhPDGF-BB) reduces Parkinsonian symptoms and increases dopamine transporter (DAT) binding in several animal models of Parkinson's disease (PD). Effects of rhPDGF-BB are the result of proliferation of ventricular wall progenitor cells and reversed by blocking mitosis. Based on these restorative effects, we assessed the safety and tolerability of intracerebroventricular (i.c.v.) rhPDGF-BB administration in individuals with PD. METHODS. We conducted a double-blind, randomized, placebo-controlled phase I/IIa study at two clinical centers in Sweden. Twelve patients with moderate PD received rhPDGF-BB via an implanted drug infusion pump and an investigational i.c.v. catheter. Patients were assigned to a dose cohort (0.2, 1.5, or 5 μg rhPDGF-BB per day) and then randomized to active treatment or placebo (3:1) for a 12-day treatment period. The primary objective was to assess safety and tolerability of i.c.v.-delivered rhPDGF-BB. Secondary outcome assessments included several clinical rating scales and changes in DAT binding. The follow-up period was 85 days. RESULTS. All patients completed the study. There were no unresolved adverse events. Serious adverse events occurred in three patients; however, these were unrelated to rhPDGF-BB administration. Secondary outcome parameters did not show dose-dependent changes in clinical rating scales, but there was a positive effect on DAT binding in the right putamen. CONCLUSION. At all doses tested, i.c.v. administration of rhPDGF-BB was well tolerated. Results support further clinical development of rhPDGF-BB for patients with PD. TRIAL REGISTRATION. Clinical Trials.gov NCT00866502. FUNDING. Newron Sweden AB (former NeuroNova AB) and Swedish Governmental Agency for Innovation Systems (VINNOVA).

  6. Fast spectroscopic variations on rapidly-rotating, cool dwarfs. 3: Masses of circumstellar absorbing clouds on AB Doradus

    NASA Technical Reports Server (NTRS)

    Cameron, A. Collier; Duncan, D. K.; Ehrenfreund, P.; Foing, B. H.; Kuntz, K. D.; Penston, M. V.; Robinson, R. D.; Soderblom, D. R.

    1989-01-01

    New time-resolved H alpha, Ca II H and K and Mg II h and k spectra of the rapidly-rotating K0 dwarf star AB Doradus (= HD 36705). The transient absorption features seen in the H alpha line are also present in the Ca II and Mg II resonance lines. New techniques are developed for measuring the average strength of the line absorption along lines of sight intersecting the cloud. These techniques also give a measure of the projected cloud area. The strength of the resonance line absorption provides useful new constraints on the column densities, projected surface areas, temperatures and internal turbulent velocity dispersions of the circumstellar clouds producing the absorption features. At any given time the star appears to be surrounded by at least 6 to 10 clouds with masses in the range 2 to 6 x 10(exp 17) g. The clouds appear to have turbulent internal velocity dispersions of order 3 to 20 km/s, comparable with the random velocities of discrete filamentary structures in solar quiescent prominences. Night-to-night changes in the amount of Ca II resonance line absorption can be explained by changes in the amplitude of turbulent motions in the clouds. The corresponding changes in the total energy of the internal motions are of order 10(exp 29) erg per cloud. Changes of this magnitude could easily be activated by the frequent energetic (approximately 10(exp 34) erg) x ray flares seen on this star.

  7. Spectroscopic confirmation of M-dwarf candidate members of the Beta Pictoris and AB Doradus Moving Groups

    NASA Astrophysics Data System (ADS)

    Binks, A. S.; Jeffries, R. D.

    2016-01-01

    Optical spectroscopic observations are reported for 24 and 23, nearby, proper-motion-selected M-dwarf candidate members of the Beta Pictoris and AB Doradus moving groups (BPMG and ABDMG). Using kinematic criteria, the presence of both Hα emission and high X-ray-to-bolometric luminosity, and position in absolute colour-magnitude diagrams, 10 and 6 of these candidates are confirmed as likely members of the BPMG and ABDMG, respectively. Equivalent widths or upper limits for the Li I 6708 Å line are reported and the lithium depletion boundary (LDB) age of the BPMG is revisited. Whilst non-magnetic evolutionary models still yield an estimated age of 21 ± 4 Myr, models that incorporate magnetic inhibition of convection imply an older age of 24 ± 4 Myr. A similar systematic increase would be inferred if the stars were 25 per cent covered by dark magnetic starspots. Since young, convective M-dwarfs are magnetically active and do have starspots, we suggest that the original LDB age estimate is a lower limit. The LDB age of the ABDMG is still poorly constrained - non-magnetic evolutionary models suggest an age in the range 35-150 Myr, which could be significantly tightened by new measurements for existing candidate members.

  8. Relating turbulent pressure and macroturbulence across the HR diagram with a possible link to γ Doradus stars

    NASA Astrophysics Data System (ADS)

    Grassitelli, L.; Fossati, L.; Langer, N.; Miglio, A.; Istrate, A. G.; Sanyal, D.

    2015-12-01

    A significant fraction of the envelope of low- and intermediate-mass stars is unstable to convection, leading to sub-surface turbulent motion. Here, we consider and include the effects of turbulence pressure in our stellar evolution calculations. In search of an observational signature, we compare the fractional contribution of turbulent pressure to the observed macroturbulent velocities in stars at different evolutionary stages. We find a strong correlation between the two quantities, similar to what was previously found for massive OB stars. We therefore argue that turbulent pressure fluctuations of finite amplitude may excite high-order, high-angular degree stellar oscillations, which manifest themselves at the surface an additional broadening of the spectral lines, i.e., macroturbulence, across most of the HR diagram. When considering the locations in the HR diagram where we expect high-order oscillations to be excited by stochastic turbulent pressure fluctuations, we find a close match with the observational γ Doradus instability strip, which indeed contains high-order, non-radial pulsators. We suggest that turbulent pressure fluctuations on a percentual level may contribute to the γ Dor phenomenon, calling for more detailed theoretical modeling in this direction. Appendices A and B are available in electronic form at http://www.aanda.org

  9. Influence of calcareous soil on Cry3Bb1 expression and efficacy in the field.

    PubMed

    Wangila, David S; Valencia J, Arnubio; Wang, Haichuan; Siegfried, Blair D; Meinke, Lance J

    2017-03-22

    Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of unexpected injury, experiments were conducted during 2013 and 2014 to ascertain whether the calcareous soil conditions and associated poor maize growth negatively affect the expression of Cry3Bb1. Quantitative determination of Cry3Bb1 protein expression levels in root tissues was carried out on plants at V5-V6 growth stage using the enzyme-linked immunosorbent assay. Cry3Bb1 and non-Bt near isoline maize hybrids were artificially infested with Cry3Bb1-susceptible WCR eggs to measure survival and efficacy of Cry3Bb1 maize in calcareous and non-calcareous soils. Results showed that there was not a significant difference in expression of Cry3Bb1 protein between plants from calcareous and non-calcareous soils (18.9-21.2 µg/g fresh weight). Western corn rootworm survival was about sevenfold greater from the non-Bt isoline than Cry3Bb1 maize indicating that Cry3Bb1 performed as expected when infested with a Cry3Bb1 susceptible rootworm population. When survival from calcareous and non-calcareous soils was compared, no significant differences were observed in each soil. A significant positive correlation between soil pH and expression of Cry3Bb1 protein in roots was detected from samples collected in 2014 but not in 2013. No such correlation was found between soil pH and survival of WCR. Results suggest that Cry3Bb1 expression levels were sufficient to provide adequate root protection against WCR regardless of soil environment, indicating that lowered Cry3Bb1 expression is not a contributing factor to the greater than expected WCR injury observed in some southwestern Nebraska

  10. Soyasaponin Bb Protects Rat Hepatocytes from Alcohol-Induced Oxidative Stress by Inducing Heme Oxygenase-1

    PubMed Central

    Lijie, Zhu; Ranran, Fu; Xiuying, Liu; Yutang, He; Bo, Wang; Tao, Ma

    2016-01-01

    Background: It has been known that oxidative stress induced by alcohol played a crucial role in the formation of alcoholic liver disease. Although the formation mechanisms underlying liver injury induced by alcohol still remained largely unknown, it has been considered that oxidative stress played a core role in the pathogenesis of hepatocyte damage. Objective: The aim of this study was to investigate the effects of soyasaponin Bb (Ss-Bb) on oxidative stress in alcohol-induced rat hepatocyte injury. Results: It has been shown that the administration of Ss-Bb could significantly restore antioxidant activity in BRL 3A cells. Moreover, the impaired liver function and morphology changes resulting from ethanol exposure were improved by Ss-Bb treatment. Treatment with a pharmacological inhibitor of haem oxygenase-1 (HO-1) indicated a critical role of HO-1 in mediating the protective role. Finally, we found that pretreatment with Ss-Bb to ethanol exposure cells increased the expression level of HO-1. Conclusion: It was suggested that Ss-Bb may protect against alcohol-induced hepatocyte injury through ameliorating oxidative stress, and the induction of HO-1 was an important protective mechanism. SUMMARY Effects of soyasaponin Bb was investigated on oxidative stress in rat hepatocytesCell viability and antioxidant capacities were evaluated to determine the effectsThe expression level of HO-1 was measured to reveal the proptective mechanisms PMID:27867273

  11. Virulence and immunity of Staphylococcus aureus BB and certain deficient mutants.

    PubMed Central

    Hasegawa, N; San Clemente, C L

    1978-01-01

    Coagulase-negative and deoxyribonuclease-negative mutants were isolated from Staphylococcus aureus BB by treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Comparison of virulence (50% lethal dose) to mice of these six mutant strains and S. aureus BB was determined by both intravenous and intraperitoneal routes. The ratios of the 50% lethal dose of coagulase-negative mutants to that of the parental strain S. aureus BB ranged from 201 to 403 for intravenous infection and 30.7 to 52.7 for intraperitoneal infection. The virulence of deoxyribonuclease-negative mutants was essentially the same as that of S. aureus BB. When mice were immunized subcutaneously with live S. aureus BB or its deoxyribonuclease-negative mutants, the resulting protection against the intravenous challenge of S. aureus BB was remarkable. The ratios of the 50% lethal dose for the mice that were immunized by these strains to that for the untreated mice extended from 40.1 to 60.6 for intravenous infection and 6.61 to 11.5 for the intraperitoneal route. However, no effect against S. aureus BB challenge was shown in the mice that were immunized with coagulase-negative mutants. PMID:730368

  12. Elevated glutamine metabolism in splenocytes from spontaneously diabetic BB rats.

    PubMed

    Wu, G Y; Field, C J; Marliss, E B

    1991-02-15

    To investigate the metabolic fates of glutamine in splenocytes from the BB rat with spontaneous immunologically mediated insulin-dependent diabetes, freshly isolated cells were incubated in Krebs-Ringer Hepes buffer with 1.0 mM-[U-14C]glutamine and 0, 4 mM- or 15 mM-glucose. (1) The major products of glutamine metabolism in splenocytes from normal and diabetic rats were ammonia, glutamate, aspartate and CO2. (2) The addition of glucose increased (P less than 0.01) glutamate production, but decreased (P less than 0.01) aspartate and CO2 production from glutamine, as compared with the values obtained in the absence of glucose. However, there were no differences in these metabolites of glutamine at 4 mM- and 15 mM-glucose. (3) At all glucose concentrations used, the productions of ammonia, glutamate, aspartate and CO2 from glutamine were all markedly increased (P less than 0.01) in splenocytes from diabetic rats. (4) Potential ATP production from glutamine in the splenocytes was similar to that from glucose, and was increased in cells from the diabetic rat. (5) ATP concentrations were increased (P less than 0.01) in diabetic-rat splenocytes in the presence of glutamine with or without glucose. (6) Our results demonstrate that glutamine is an important energy substrate for splenocytes and suggest that the increased glutamine metabolism may be associated with the activation of certain subsets of splenocytes in the immunologically mediated diabetic syndrome.

  13. The TT, TB, EB and BB correlations in anisotropic inflation

    SciTech Connect

    Chen, Xingang; Emami, Razieh; Firouzjahi, Hassan; Wang, Yi E-mail: emami@ipm.ir E-mail: yw366@cam.ac.uk

    2014-08-01

    The ongoing and future experiments will measure the B-mode from different sky coverage and frequency bands, with the potential to reveal non-trivial features in polarization map. In this work we study the TT, TB, EB and BB correlations associated with the B-mode polarization of CMB map in models of charged anisotropic inflation. The model contains a chaotic-type large field complex inflaton which is charged under the U(1) gauge field. We calculate the statistical anisotropies generated in the power spectra of the curvature perturbation, the tensor perturbation and their cross-correlation. It is shown that the asymmetry in tensor power spectrum is a very sensitive probe of the gauge coupling. While the level of statistical anisotropy in temperature power spectrum can be small and satisfy the observational bounds, the interactions from the gauge coupling can induce large directional dependence in tensor modes. This will leave interesting anisotropic fingerprints in various correlations involving the B-mode polarization such as the TB cross-correlation which may be detected in upcoming Planck polarization data. In addition, the TT correlation receives an anisotropic contribution from the tensor sector which naturally decays after l ∼> 100. We expect that the mechanism of using tensor sector to induce asymmetry at low l to be generic which can also be applied to address other low l CMB anomalies.

  14. Bifidobacterium animalis subsp. lactis BB-12 in reducing the risk of infections in infancy.

    PubMed

    Taipale, Teemu; Pienihäkkinen, Kaisu; Isolauri, Erika; Larsen, Charlotte; Brockmann, Elke; Alanen, Pentti; Jokela, Jorma; Söderling, Eva

    2011-02-01

    The impact of controlled administration of Bifidobacterium animalis subsp. lactis BB-12 (BB-12) on the risk of acute infectious diseases was studied in healthy newborn infants. In this double-blind, placebo-controlled study, 109 newborn 1-month-old infants were assigned randomly to a probiotic group receiving a BB-12-containing tablet (n 55) or to a control group receiving a control tablet (n 54). Test tablets were administered to the infants twice a day (daily dose of BB-12 10 billion colony-forming units) from the age of 1-2 months to 8 months with a novel slow-release pacifier or a spoon. Breastfeeding habits, pacifier use, dietary habits, medications and all signs and symptoms of acute infections were registered. At the age of 8 months, faecal samples were collected for BB-12 determination (quantitative PCR method). The baseline characteristics of the two groups were similar, as was the duration of exclusive breastfeeding. BB-12 was recovered (detection limit log 5) in the faeces of 62% of the infants receiving the BB-12 tablet. The daily duration of pacifier sucking was not associated with the occurrence of acute otitis media. No significant differences between the groups were observed in reported gastrointestinal symptoms, otitis media or use of antibiotics. However, the infants receiving BB-12 were reported to have experienced fewer respiratory infections (65 v. 94%; risk ratio 0·69; 95% CI 0·53, 0·89; P = 0·014) than the control infants. Controlled administration of BB-12 in early childhood may reduce respiratory infections.

  15. First results from the faint object camera - High-resolution observations of the central object R136 in the 30 Doradus nebula

    NASA Technical Reports Server (NTRS)

    Weigelt, G.; Albrecht, R.; Barbieri, C.; Blades, J. C.; Boksenberg, A.; Crane, P.; Deharveng, J. M.; Disney, M. J.; Jakobsen, P.; Kamperman, T. M.

    1991-01-01

    R136 is the luminous central object of the giant H II region 30 Doradus in the LMC. The first high-resolution observations of R136 with the Faint Object Camera on board the Hubble Space Telescope are reported. The physical nature of the brightest component R136a has been a matter of some controversy over the last few years. The UV images obtained show that R136a is a very compact star cluster consisting of more than eight stars within 0.7 arcsec diameter. From these high-resolution images a mass upper limit can be derived for the most luminous stars observed in R136.

  16. Ionization Structure in the 30 Doradus Nebula as seen with Hubble Space Telescope Wide Field Planetary Camera 2

    NASA Astrophysics Data System (ADS)

    Scowen, P. A.; Hester, J. J.; Sankrit, R.; Gallagher, J. S.; Ballester, G. E.; Burrows, C. J.; Clarke, J. T.; Crisp, D.; Evans, R. W.; Griffiths, R. E.; Hoessel, J. G.; Holtzman, J. A.; Krist, J.; Mould, J. R.; Stapelfeldt, K. R.; Trauger, J. T.; Watson, A. M.; Westphal, J. A.

    1998-07-01

    Using the Hubble Space Telescope (HST) and Wide Field Planetary Camera 2, we have imaged the central 20 pc of the giant H ii region 30 Doradus Nebula in three different emission lines. The images allow us to study the nebula with a physical resolution that is within a factor of 2 of that of typical ground-based observations of Galactic H ii regions. We present a gallery of interesting objects within the region studied. These include a tube blown by the wind of a high-velocity star and a discrete H ii region around an isolated B star. This small isolated H ii region appears to be in the midst of the champagne flow phase of its evolution. Most of the emission within 30 Dor is confined to a thin zone located between the hot interior of the nebula and surrounding dense molecular material. This zone appears to be directly analogous to the photoionized photoevaporative flows that dominate emission from small, nearby H ii regions. For example, a column of material protruding from the cavity wall to the south of the main cluster is found to be a direct analog to elephant trunks in M16. Surface brightness profiles across this structure are very similar to surface brightness profiles taken at ground-based resolution across the head of the largest column in M16. The dynamical effects of the photoevaporative flow can be seen as well. An arcuate feature located above this column and a similar feature surrounding a second nearby column are interpreted as shocks in which the photoevaporative flow stagnates against the high-temperature gas that fills the majority of the nebula. The ram pressure in the photoevaporative flow, derived from thermal pressure at the surface of the column, is found to balance with the pressure in the interior of the nebula derived from previous X-ray observations. By analogy with the comparison of ground-based and HST images of M16, we infer that the same sharply stratified structure seen in HST images of M16 almost certainly underlies the observed

  17. HYBRID {gamma} DORADUS-{delta} SCUTI PULSATORS: NEW INSIGHTS INTO THE PHYSICS OF THE OSCILLATIONS FROM KEPLER OBSERVATIONS

    SciTech Connect

    Grigahcene, A.; Monteiro, M. J. P. F. G.; Antoci, V.; Handler, G.; Houdek, G.; Balona, L.; Catanzaro, G.; Daszynska-Daszkiewicz, J.; Guzik, J. A.; Kurtz, D. W.; Marconi, M.; Ripepi, V.; Moya, A.; Suarez, J.-C.; Uytterhoeven, K.; Brown, T. M.; Christensen-Dalsgaard, J.; Gilliland, R. L.; Jenkins, J. M.

    2010-04-20

    Observations of the pulsations of stars can be used to infer their interior structure and test theoretical models. The main-sequence {gamma} Doradus (Dor) and {delta} Scuti (Sct) stars with masses 1.2-2.5 M {sub sun} are particularly useful for these studies. The {gamma} Dor stars pulsate in high-order g-modes with periods of order 1 day, driven by convective blocking at the base of their envelope convection zone. The {delta} Sct stars pulsate in low-order g- and p-modes with periods of order 2 hr, driven by the {kappa} mechanism operating in the He II ionization zone. Theory predicts an overlap region in the Hertzsprung-Russell diagram between instability regions, where 'hybrid' stars pulsating in both types of modes should exist. The two types of modes with properties governed by different portions of the stellar interior provide complementary model constraints. Among the known {gamma} Dor and {delta} Sct stars, only four have been confirmed as hybrids. Now, analysis of combined Quarter 0 and Quarter 1 Kepler data for hundreds of variable stars shows that the frequency spectra are so rich that there are practically no pure {delta} Sct or {gamma} Dor pulsators, i.e., essentially all of the stars show frequencies in both the {delta} Sct and the {gamma} Dor frequency range. A new observational classification scheme is proposed that takes into account the amplitude as well as the frequency and is applied to categorize 234 stars as {delta} Sct, {gamma} Dor, {delta} Sct/{gamma} Dor or {gamma} Dor/{delta} Sct hybrids.

  18. A Study of the Relation between Star Formation and Molecular Clumps on Subparsec Scales in 30 Doradus

    NASA Astrophysics Data System (ADS)

    Nayak, O.; Meixner, M.; Indebetouw, R.; De Marchi, G.; Koekemoer, A.; Panagia, N.; Sabbi, E.

    2016-11-01

    We present 12CO and 13CO molecular gas data observed by ALMA, massive early-stage young stellar objects (YSOs) identified by applying color-magnitude cuts to Spitzer and Herschel photometry, and low-mass late-stage YSOs identified via {{H}}α excess. Using dendrograms, we derive properties for the molecular cloud structures. This is the first time a dendrogram analysis has been applied to extragalactic clouds. The majority of clumps have a virial parameter equal to unity or less. The size-linewidth relations of 12CO and 13CO show the clumps in this study have a larger linewidth for a given size (by factors of 3.8 and 2.5, respectively) in comparison to several, but not all, previous studies. The larger linewidths in 30 Doradus compared to typical Milky Way quiescent clumps are probably due to the former’s highly energetic environmental conditions. The slopes of the size-linewidth relations of 12CO, 0.65 ± 0.04, and 13CO, 0.97 ± 0.12, are on the higher end but consistent within 3σ of those of previous studies. Massive star formation occurs in clumps with high masses (>1.83 × 102 M ⊙), high linewidths (v > 1.18 km s-1), and high mass densities (>6.67 × 102 M ⊙ pc-2). The majority of embedded, massive YSOs are associated with a clump; however, the majority of more evolved, low-mass YSOs are not.

  19. 30 Doradus - Relating Young Stars Imaged by Spitzer and Hubble to the CO Molecular Gas Observed with ALMA

    NASA Astrophysics Data System (ADS)

    Nayak, Omnarayani; Meixner, Margaret; Indebetouw, Remy; Sabbi, Elena; De Marchi, Guido; Panagia, Nino

    2016-01-01

    The majority of star have masses less than 8 solar mass and form in clumps that are less than 1 pc in size. The sub-parsec scales in which star formation takes place makes it difficult to resolve the effects star formation and the surrounding dense gas have on each other. The Magellanic Clouds are more active in forming high mass stars as compared to the Milky Way. The SAGE and Heritage surveys combined with the Hubble Tarantula Treasury Project provide us the opportunity to study high-mass (>15 solar masses) and low-mass (<1 solar mass) star formation. ALMA observations cover a 60 pc x 30 pc region of CO gas slightly north of the R136 cluster in 30 Doradus. We find 16 young stellar objects and about a 100 pre-main-sequence stars within the ALMA footprint. We define young stellar objects to be very early stage stars that are about 10,000 years old and whose SEDs peak in the infrared, and we use pre-main-sequence-stars to refer to slightly older stars that can be seen in the optical. I will use dendrograms to analyze both the high- and low-mass star properties with respect to the CO gas structure observed with ALMA. Preliminary results show that not all massive young stellar objects are associated with CO gas, higher mass clumps tend to form higher mass stars and are more likely to have multiple young stars, and lower mass clumps tend to not be gravitationally bound however the larger clouds are bound. Looking at the interplay between dense molecular gas and the newly forming stars in a stellar nursery will shed light on how these stars formed: monolithic collapse or competitive accretion.

  20. Spectroscopic Classification of ASASSN-15bb as a Type II Supernova

    NASA Astrophysics Data System (ADS)

    Milisavljevic, D.; Villar, V. A.; Lunnan, R.; Drout, M.; Patnaude, D.; Berger, E.

    2015-01-01

    A low resolution (R ~ 2000) optical spectrum (range 4200-9400 Angstroms) of ASASSN-15bb (ATel # 6936) was obtained January 17.4 UT with the Walter Baade Magellan 6.5m telescope (+ IMACS) at Las Campanas Observatory.

  1. 56. Entry door (closed), BB16, basement level, building 500, looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. Entry door (closed), BB-16, basement level, building 500, looking southeast - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

  2. Hypothetical Protein BB0569 Is Essential for Chemotaxis of the Lyme Disease Spirochete Borrelia burgdorferi

    PubMed Central

    Zhang, Kai; Liu, Jun; Charon, Nyles W.

    2015-01-01

    ABSTRACT The Lyme disease spirochete Borrelia burgdorferi has five putative methyl-accepting chemotaxis proteins (MCPs). In this report, we provide evidence that a hypothetical protein, BB0569, is essential for the chemotaxis of B. burgdorferi. While BB0569 lacks significant homology to the canonical MCPs, it contains a conserved domain (spanning residues 110 to 170) that is often evident in membrane-bound MCPs such as Tar and Tsr of Escherichia coli. Unlike Tar and Tsr, BB0569 lacks transmembrane regions and recognizable HAMP and methylation domains and is similar to TlpC, a cytoplasmic chemoreceptor of Rhodobacter sphaeroides. An isogenic mutant of BB0569 constantly runs in one direction and fails to respond to attractants, indicating that BB0569 is essential for chemotaxis. Immunofluorescence, green fluorescent protein (GFP) fusion, and cryo-electron tomography analyses demonstrate that BB0569 localizes at the cell poles and is required for chemoreceptor clustering at the cell poles. Protein cross-linking studies reveal that BB0569 forms large protein complexes with MCP3, indicative of its interactions with other MCPs. Interestingly, analysis of B. burgdorferi mcp mutants shows that inactivation of either mcp2 or mcp3 reduces the level of BB0569 substantially and that such a reduction is caused by protein turnover. Collectively, these results demonstrate that the domain composition and function of BB0569 are similar in some respects to those of TlpC but that these proteins are different in their cellular locations, further highlighting that the chemotaxis of B. burgdorferi is unique and different from the Escherichia coli and Salmonella enterica paradigm. IMPORTANCE Spirochete chemotaxis differs substantially from the Escherichia coli and Salmonella enterica paradigm, and the basis for controlling the rotation of the bundles of periplasmic flagella at each end of the cell is unknown. In recent years, Borrelia burgdorferi, the causative agent of Lyme disease, has

  3. Radioimmunoassay measurement of creatine kinase bb in the serum of schizophrenic patients

    SciTech Connect

    Lerner, M.H.; Friedhoff, A.J.

    1980-03-03

    Brain type creatine kinase (BB) isoenzyme was measured using a highly sensitive and specific radioimmunoassay procedure in two schizophrenic populations. The data would indicate that in the schizophrenic populations examined there is insufficient tissue disruption to cause abnormal build-up of brain creatine kinase levels. However the possibility of a rapid removal of creatine kinase BB from the circulation exists. The elevated creatine kinase reported in acute schizophrenics is most likely not of brain origin.

  4. Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB

    SciTech Connect

    Mustoe, T.A.; Purdy, J.; Gramates, P.; Deuel, T.F.; Thomason, A.; Pierce, G.F. )

    1989-10-01

    This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rads total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p less than 0.005) and day 12 (p less than 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.

  5. A fibronectin peptide redirects PDGF-BB/PDGFR complexes to macropinocytosis-like internalization and augments PDGF-BB survival signals

    PubMed Central

    Zhu, Jia; Lin, Fubao; Brown, Deborah A.; Clark, Richard A.F.

    2013-01-01

    Growth factor-binding domains identified in various extracellular matrix (ECM) proteins have been shown to regulate growth factor activity in many ways. Recently we identified a fibronectin peptide (P12) that can bind platelet-derived growth factor BB (PDGF-BB) and promote adult human dermal fibroblast (AHDF) survival under stress. In vivo experiments in a porcine burn injury model showed that P12 limited burn injury progression, suggesting an active role in tissue survival. In this report, we explored the molecular mechanism of this peptide in ADHF under nutrient deprivation. Our results showed that P12 acted like some cell penetrating peptides (CPPs) in that it redirected ligand-bound PDGFR from the clathrin-dependent endocytic pathway to a slower, macropinocytosis-like pathway. P12 slowed internalization and degradation of PDGF-BB, augmented its survival signals, and promoted cell survival after nutrient-removal. Our findings demonstrate a mechanism for a potential therapeutic peptide that increases cell and tissue survival by acting as a cofactor to PDGF-BB. PMID:24304816

  6. Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin.

    PubMed

    Michalska, Marta; Kozakiewicz, Marcin; Bodek, Andrzej; Bodek, Kazimiera Henryka

    2015-04-01

    The effect of homogeneous fibrin (Fb), collagen (Coll) and composite fibrin-heparin (Fb-Hp), fibrin-collagen (Fb-Coll) membranes on in vitro release of platelet-derived growth factor (PDGF-BB) was evaluated in the presence or absence of amoxicillin using of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 272 nm. The process of the PDGF-BB growth factor and amoxicillin release from the studied membranes was of a two-phase nature in the majority of the systems analysed. The PDGF-BB was released in the highest amount from the Coll membrane (M7) without the presence of amoxicillin--546.2 ± 7.47 pg, t0.5 = 0.88 h and 202.5 ± 6.83 pg, t0.5 = 26.65 h during the first phase and second phase, respectively. The lowest PDGF-BB release was observed from composite M4 (Fb-Hp) membrane--5.88 ± 0.81 pg, t0.5 = 1.69 h; and 110.2 ± 6.48 pg, t0.5 = 855.6 h during first and second phase respectively. An optimal release of amoxicillin was observed in the case of the composite M6 (Fb-Coll) membrane--only in the second phase: 64.2 ± 7.8 μg, t0.5 = 83.5 h. The lowest and delayed amoxicillin release was achieved for M4 membrane (approx. 17.1 ± 1.12 μg, t0.5 = 46.5 h). The results of the PDGF-BB release and amoxicillin from membranes indicated a correlation between the level of release and composition of the film. Our results suggested that fibrin and collagen membranes may be beneficial to enhance periodontal bone regeneration.

  7. Interior rotation of a sample of γ Doradus stars from ensemble modelling of their gravity-mode period spacings

    NASA Astrophysics Data System (ADS)

    Van Reeth, T.; Tkachenko, A.; Aerts, C.

    2016-10-01

    Context. Gamma Doradus stars (hereafter γ Dor stars) are known to exhibit gravity- and/or gravito-intertial modes that probe the inner stellar region near the convective core boundary. The non-equidistant spacing of the pulsation periods is an observational signature of the stellar evolutions and current internal structure and is heavily influenced by rotation. Aims: We aim to constrain the near-core rotation rates for a sample of γ Dor stars for which we have detected period spacing patterns. Methods: We combined the asymptotic period spacing with the traditional approximation of stellar pulsation to fit the observed period spacing patterns using χ2-optimisation. The method was applied to the observed period spacing patterns of a sample of stars and used for ensemble modelling. Results: For the majority of stars with an observed period spacing pattern we successfully determined the rotation rates and the asymptotic period spacing values, although the uncertainty margins on the latter were typically large. This also resulted directly in the identification of the modes that correspond to the detected pulsation frequencies, which for most stars were prograde dipole gravity and gravito-inertial modes. The majority of the observed retrograde modes were found to be Rossby modes. We also discuss the limitations of the method that are due to the neglect of the centrifugal force and the incomplete treatment of the Coriolis force. Conclusions: Despite its current limitations, the proposed method was successful to derive the rotation rates and to identify the modes from the observed period spacing patterns. It forms the first step towards detailed seismic modelling based on observed period spacing patterns of moderately to rapidly rotating γDor stars. Based on data gathered with the NASA Discovery mission Kepler and the HERMES spectrograph, which is installed at the Mercator Telescope, operated on the island of La Palma by the Flemish Community at the Spanish

  8. THE RELATIONSHIP OF LONGITUDINAL LEVELS OF COMPLEMENT Bb DURING PREGNANCY WITH PREECLAMPSIA

    PubMed Central

    Lynch, Anne M.; Wagner, Brandie D.; Giclas, Patricia C.; West, Nancy A.; Gibbs, Ronald S.; Holers, V. Michael

    2015-01-01

    PROBLEM To determine the understudied relationship between Bb during pregnancy in subjects with preeclampsia compared with normotensive controls. METHOD OF STUDY Nested case-control study. RESULTS Average Bb levels significantly decreased over time in pregnancy (weekly slope (SE): −0.0094 (0.0005), p < 0.01). Cross-sectionally, at less than 10 weeks, Bb levels decreased with increasing gestational age in women who remained normotensive (weekly slope (SE): −0.007 (0.02) and for women who developed preeclampsia (weekly slope (SE): −0.059 (0.03) p = 0.12). Among women who developed preeclampsia, Bb levels were greatest when samples were drawn in the gestational window of 15–20 weeks [(weekly slope (SE): 0.06 (0.02)] while levels among normotensive women were inversely related with gestational age [weekly slope (SE): −0.02 (0.01)]. The differences in slopes between cases and controls between 10 and 21 weeks’ gestation was statistically significant (p = 0.003). CONCLUSIONS We suggest dysregulation of Bb activation between 10 and 20 weeks’ gestation in women who develop preeclampsia. PMID:26510395

  9. Prognostic value of creatine kinase BB-isoenzyme in high risk newborn infants.

    PubMed Central

    Ruth, V J

    1989-01-01

    Serum creatine kinase BB-isoenzyme (CK-BB) activity was studied on the first day of life in 31 acutely asphyxiated infants, 70 infants born after high risk pregnancies (pre-eclampsia or intrauterine growth retardation, or both), and 47 very low birthweight infants. Neuro-developmental evaluation was carried out at 2.2-2.5 years. Eight infants died with, and eight without, hypoxic-ischaemic lesions of the brain, 14 had cerebral palsy, 16 had mild motor impairment, six had developmental delay without motor impairment, and 96 were normal at follow up. Infants who died with brain injury had significantly higher CK-BB activity than infants with normal outcomes (geometric mean 12 U/l); the mean difference was 82 U/l with a 95% confidence interval from 31 to 219 U/l. CK-BB in infants with cerebral palsy and mild motor impairment (geometric means 12 and 15 U/l, respectively) were similar to controls. CK-BB activity after birth is predictive of neonatal death but not of neurological damage in survivors. PMID:2751331

  10. Testis-Specific Bb8 Is Essential in the Development of Spermatid Mitochondria

    PubMed Central

    Vedelek, Viktor; Laurinyecz, Barbara; Kovács, Attila L.; Juhász, Gábor

    2016-01-01

    Mitochondria are essential organelles of developing spermatids in Drosophila, which undergo dramatic changes in size and shape after meiotic division, where mitochondria localized in the cytoplasm, migrate near the nucleus, aggregate, fuse and create the Nebenkern. During spermatid elongation the two similar mitochondrial derivatives of the Nebenkern start to elongate parallel to the axoneme. One of the elongated mitochondrial derivatives starts to lose volume and becomes the minor mitochondrial derivative, while the other one accumulates paracrystalline and becomes the major mitochondrial derivative. Proteins and intracellular environment that are responsible for cyst elongation and paracrystalline formation in the major mitochondrial derivative need to be identified. In this work we investigate the function of the testis specific big bubble 8 (bb8) gene during spermatogenesis. We show that a Minos element insertion in bb8 gene, a predicted glutamate dehydrogenase, causes recessive male sterility. We demonstrate bb8 mRNA enrichment in spermatids and the mitochondrial localisation of Bb8 protein during spermatogenesis. We report that megamitochondria develop in the homozygous mutant testes, in elongating spermatids. Ultrastructural analysis of the cross section of elongated spermatids shows enlarged mitochondria and the production of paracrystalline in both major and minor mitochondrial derivatives. Our results suggest that the Bb8 protein and presumably glutamate metabolism has a crucial role in the normal development and establishment of the identity of the mitochondrial derivatives during spermatid elongation. PMID:27529784

  11. THE HIGH-METALLICITY EXPLOSION ENVIRONMENT OF THE RELATIVISTIC SUPERNOVA 2009bb

    SciTech Connect

    Levesque, E. M.; Kewley, L. J.; Soderberg, A. M.; Foley, R. J.; Berger, E.; Torres, M. A. P.; Challis, P.; Kirshner, R. P.; Copete, A.; Chakraborti, S.; Ray, A.; Barthelmy, S. D.; Bietenholz, M. F.; Chandra, P.; Chaplin, V.; Connaughton, V.; Chevalier, R. A.; Fox, O.; Chugai, N.; Fransson, C.

    2010-01-20

    We investigate the environment of the nearby (d {approx} 40 Mpc) broad-lined Type Ic supernova (SN) 2009bb. This event was observed to produce a relativistic outflow likely powered by a central accreting compact object. While such a phenomenon was previously observed only in long-duration gamma-ray bursts (LGRBs), no LGRB was detected in association with SN 2009bb. Using an optical spectrum of the SN 2009bb explosion site, we determine a variety of interstellar medium properties for the host environment, including metallicity, young stellar population age, and star formation rate. We compare the SN explosion site properties to observations of LGRB and broad-lined SN Ic host environments on optical emission line ratio diagnostic diagrams. Based on these analyses, we find that the SN 2009bb explosion site has a metallicity between 1.7 Z {sub sun} and 3.5 Z {sub sun}, in agreement with other broad-lined SN Ic host environments and at odds with the low-redshift LGRB host environments and recently proposed maximum metallicity limits for relativistic explosions. We consider the implications of these findings and the impact that SN 2009bb's unusual explosive properties and environment have on our understanding of the key physical ingredient that enables some SNe to produce a relativistic outflow.

  12. Evaluation of Borrelia burgdorferi BbHtrA Protease as a Vaccine Candidate for Lyme Borreliosis in Mice.

    PubMed

    Ullmann, Amy J; Russell, Theresa M; Dolan, Marc C; Williams, Martin; Hojgaard, Andrias; Weiner, Zachary P; Johnson, Barbara J B

    2015-01-01

    Borrelia burgdorferi synthesizes an HtrA protease (BbHtrA) which is a surface-exposed, conserved protein within Lyme disease spirochetes with activity toward CheX and BmpD of Borrelia spp, as well as aggrecan, fibronectin and proteoglycans found in skin, joints and neural tissues of vertebrates. An antibody response against BbHtrA is observed in Lyme disease patients and in experimentally infected laboratory mice and rabbits. Given the surface location of BbHtrA on B. burgdorferi and its ability to elicit an antibody response in infected hosts, we explored recombinant BbHtrA as a potential vaccine candidate in a mouse model of tick-transmitted Lyme disease. We immunized mice with two forms of BbHtrA: the proteolytically active native form and BbHtrA ablated of activity by a serine to alanine mutation at amino acid 226 (BbHtrA(S226A)). Although inoculation with either BbHtrA or BbHtrA(S226A) produced high-titer antibody responses in C3H/HeJ mice, neither antigen was successful in protecting mice from B. burgdorferi challenge. These results indicate that the search for novel vaccine candidates against Lyme borreliosis remains a challenge.

  13. Dissecting 30 Doradus: Optical and Near Infrared Star Formation History of the starburst cluster NGC2070 from the Hubble Tarantula Treasury Project

    NASA Astrophysics Data System (ADS)

    Cignoni, Michele

    2015-08-01

    I will present new results on the star formation history of 30 Doradus in the Large Magellanic Cloud based on the panchromatic imaging survey Hubble Tarantula Treasury Project (HTTP). Here the focus is on the starburst cluster NGC2070. The star formation history is derived by comparing the deepest ever optical and NIR color-magnitude diagrams (CMDs) with state-of-the-art synthetic CMDs generated with the latest PARSEC models, which include all stellar phases from pre-main sequence (PMS) to post-main sequence. For the first time in this region we are able to measure the star formation using intermediate and low mass stars simultaneously. Our results suggest that NGC2070 experienced a prolonged activity. I will discuss the detailed star formation history, initial mass function and reddening distribution and how these relate to previous studies of this starburst region.

  14. Dissecting 30 Doradus: Optical and Near Infrared Star Formation History of the starburst cluster NGC2070 from the Hubble Tarantula Treasury Project

    NASA Astrophysics Data System (ADS)

    Cignoni, Michele; HTTP Team

    2017-03-01

    I will present new results on the star formation history of 30 Doradus in the Large Magellanic Cloud based on the panchromatic imaging survey Hubble Tarantula Treasury Project (HTTP). Here the focus is on the starburst cluster NGC2070. The star formation history is derived by comparing the deepest ever optical and NIR color-magnitude diagrams (CMDs) with state-of-the-art synthetic CMDs generated with the latest PARSEC models, which include all stellar phases from pre-main sequence to post-main sequence. For the first time in this region we are able to measure the star formation using intermediate and low mass stars simultaneously. Our results suggest that NGC2070 experienced a prolonged activity. I will discuss the detailed star formation history, initial mass function and reddening distribution.

  15. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... 63.7(f) Alternative Test Method Yes. 63.7(g) Data Analysis Yes. 63.7(h) Waiver of Tests Yes. 63.8(a...) Data Reduction Yes. 63.8(g)(2) No Subpart BB does not require COMS or CEMS. 63.8(g)(3) through (5) Yes... additional requirements. A CMS performance report is not required. 63.10(e)(4) COMS Data Reports No...

  16. Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor.

    PubMed

    Clements, J M; Beckett, R P; Brown, A; Catlin, G; Lobell, M; Palan, S; Thomas, W; Whittaker, M; Wood, S; Salama, S; Baker, P J; Rodgers, H F; Barynin, V; Rice, D W; Hunter, M G

    2001-02-01

    Peptide deformylase (PDF) is an essential bacterial metalloenzyme which deformylates the N-formylmethionine of newly synthesized polypeptides and as such represents a novel target for antibacterial chemotherapy. To identify novel PDF inhibitors, we screened a metalloenzyme inhibitor library and identified an N-formyl-hydroxylamine derivative, BB-3497, and a related natural hydroxamic acid antibiotic, actinonin, as potent and selective inhibitors of PDF. To elucidate the interactions that contribute to the binding affinity of these inhibitors, we determined the crystal structures of BB-3497 and actinonin bound to Escherichia coli PDF at resolutions of 2.1 and 1.75 A, respectively. In both complexes, the active-site metal atom was pentacoordinated by the side chains of Cys 90, His 132, and His 136 and the two oxygen atoms of N-formyl-hydroxylamine or hydroxamate. BB-3497 had activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis, and activity against some gram-negative bacteria. Time-kill analysis showed that the mode of action of BB-3497 was primarily bacteriostatic. The mechanism of resistance was via mutations within the formyltransferase gene, as previously described for actinonin. While actinonin and its derivatives have not been used clinically because of their poor pharmacokinetic properties, BB-3497 was shown to be orally bioavailable. A single oral dose of BB-3497 given 1 h after intraperitoneal injection of S. aureus Smith or methicillin-resistant S. aureus protected mice from infection with median effective doses of 8 and 14 mg/kg of body weight, respectively. These data validate PDF as a novel target for the design of a new generation of antibacterial agents.

  17. 4-1BB Agonists: Multi-Potent Potentiators of Tumor Immunity

    PubMed Central

    Bartkowiak, Todd; Curran, Michael A.

    2015-01-01

    Immunotherapy is a rapidly expanding field of oncology aimed at targeting, not the tumor itself, but the immune system combating the cancerous lesion. Of the many approaches currently under study to boost anti-tumor immune responses; modulation of immune co-receptors on lymphocytes in the tumor microenvironment has thus far proven to be the most effective. Antibody blockade of the T cell co-inhibitory receptor cytotoxic T lymphocyte antigen-4 (CTLA-4) has become the first FDA approved immune checkpoint blockade; however, tumor infiltrating lymphocytes express a diverse array of additional stimulatory and inhibitory co-receptors, which can be targeted to boost tumor immunity. Among these, the co-stimulatory receptor 4-1BB (CD137/TNFSF9) possesses an unequaled capacity for both activation and pro-inflammatory polarization of anti-tumor lymphocytes. While functional studies of 4-1BB have focused on its prominent role in augmenting cytotoxic CD8 T cells, 4-1BB can also modulate the activity of CD4 T cells, B cells, natural killer cells, monocytes, macrophages, and dendritic cells. 4-1BB’s expression on both T cells and antigen presenting cells, coupled with its capacity to promote survival, expansion, and enhanced effector function of activated T cells, has made it an alluring target for tumor immunotherapy. In contrast to immune checkpoint blocking antibodies, 4-1BB agonists can both potentiate anti-tumor and anti-viral immunity, while at the same time ameliorating autoimmune disease. Despite this, 4-1BB agonists can trigger high grade liver inflammation which has slowed their clinical development. In this review, we discuss how the underlying immunobiology of 4-1BB activation suggests the potential for therapeutically synergistic combination strategies in which immune adverse events can be minimized. PMID:26106583

  18. Hubble Tarantula Treasury Project. III. Photometric Catalog and Resulting Constraints on the Progression of Star Formation in the 30 Doradus Region

    NASA Astrophysics Data System (ADS)

    Sabbi, E.; Lennon, D. J.; Anderson, J.; Cignoni, M.; van der Marel, R. P.; Zaritsky, D.; De Marchi, G.; Panagia, N.; Gouliermis, D. A.; Grebel, E. K.; Gallagher, J. S., III; Smith, L. J.; Sana, H.; Aloisi, A.; Tosi, M.; Evans, C. J.; Arab, H.; Boyer, M.; de Mink, S. E.; Gordon, K.; Koekemoer, A. M.; Larsen, S. S.; Ryon, J. E.; Zeidler, P.

    2016-01-01

    We present and describe the astro-photometric catalog of more than 800,000 sources found in the Hubble Tarantula Treasury Project (HTTP). HTTP is a Hubble Space Telescope Treasury program designed to image the entire 30 Doradus region down to the sub-solar (˜0.5 M⊙) mass regime using the Wide Field Camera 3 and the Advanced Camera for Surveys. We observed 30 Doradus in the near-ultraviolet (F275W, F336W), optical (F555W, F658N, F775W), and near-infrared (F110W, F160W) wavelengths. The stellar photometry was measured using point-spread function fitting across all bands simultaneously. The relative astrometric accuracy of the catalog is 0.4 mas. The astro-photometric catalog, results from artificial star experiments, and the mosaics for all the filters are available for download. Color-magnitude diagrams are presented showing the spatial distributions and ages of stars within 30 Dor as well as in the surrounding fields. HTTP provides the first rich and statistically significant sample of intermediate- and low-mass pre-main sequence candidates and allows us to trace how star formation has been developing through the region. The depth and high spatial resolution of our analysis highlight the dual role of stellar feedback in quenching and triggering star formation on the giant H ii region scale. Our results are consistent with stellar sub-clustering in a partially filled gaseous nebula that is offset toward our side of the Large Magellanic Cloud. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA Inc., under NASA contract NAS 5-26555.

  19. HUBBLE TARANTULA TREASURY PROJECT. III. PHOTOMETRIC CATALOG AND RESULTING CONSTRAINTS ON THE PROGRESSION OF STAR FORMATION IN THE 30 DORADUS REGION

    SciTech Connect

    Sabbi, E.; Anderson, J.; Cignoni, M.; Marel, R. P. van der; Panagia, N.; Sana, H.; Aloisi, A.; Arab, H.; Gordon, K.; Lennon, D. J.; Zaritsky, D.; Marchi, G. De; Gouliermis, D. A.; Grebel, E. K.; III, J. S. Gallagher; Smith, L. J.; Evans, C. J.; Boyer, M.; Mink, S. E. de; and others

    2016-01-15

    We present and describe the astro-photometric catalog of more than 800,000 sources found in the Hubble Tarantula Treasury Project (HTTP). HTTP is a Hubble Space Telescope Treasury program designed to image the entire 30 Doradus region down to the sub-solar (∼0.5 M{sub ⊙}) mass regime using the Wide Field Camera 3 and the Advanced Camera for Surveys. We observed 30 Doradus in the near-ultraviolet (F275W, F336W), optical (F555W, F658N, F775W), and near-infrared (F110W, F160W) wavelengths. The stellar photometry was measured using point-spread function fitting across all bands simultaneously. The relative astrometric accuracy of the catalog is 0.4 mas. The astro-photometric catalog, results from artificial star experiments, and the mosaics for all the filters are available for download. Color–magnitude diagrams are presented showing the spatial distributions and ages of stars within 30 Dor as well as in the surrounding fields. HTTP provides the first rich and statistically significant sample of intermediate- and low-mass pre-main sequence candidates and allows us to trace how star formation has been developing through the region. The depth and high spatial resolution of our analysis highlight the dual role of stellar feedback in quenching and triggering star formation on the giant H ii region scale. Our results are consistent with stellar sub-clustering in a partially filled gaseous nebula that is offset toward our side of the Large Magellanic Cloud.

  20. Study of BB ¯*/DD ¯* bound states in a Bethe-Salpeter approach

    NASA Astrophysics Data System (ADS)

    He, Jun

    2014-10-01

    In this work the BB ¯*/DD ¯* system is studied in the Bethe-Salpeter approach with quasipotential approximation. In our calculation both direct and cross diagrams are included in the one-boson-exchange potential. The numerical results indicate the existence of an isoscalar bound state DD ¯* with JPC=1++, which may be related to the X(3872). In the isovector sector, no bound state is produced from the interactions of DD ¯* and BB ¯*, which suggests the molecular state explanations for Zb(10610) and Zc(3900) are excluded.

  1. Study of the inner dust envelope and stellar photosphere of the AGB star R Doradus using SPHERE/ZIMPOL

    NASA Astrophysics Data System (ADS)

    Khouri, T.; Maercker, M.; Waters, L. B. F. M.; Vlemmings, W. H. T.; Kervella, P.; de Koter, A.; Ginski, C.; De Beck, E.; Decin, L.; Min, M.; Dominik, C.; O'Gorman, E.; Schmid, H.-M.; Lombaert, R.; Lagadec, E.

    2016-06-01

    Context. On the asymptotic giant branch (AGB) low- and intermediate-mass stars eject a large fraction of their envelope, but the mechanism driving these outflows is still poorly understood. For oxygen-rich AGB stars, the wind is thought to be driven by radiation pressure caused by scattering of radiation off dust grains. Aims: We study the photosphere, the warm molecular layer, and the inner wind of the close-by oxygen-rich AGB star R Doradus. We focus on investigating the spatial distribution of the dust grains that scatter light and whether these grains can be responsible for driving the outflow of this star. Methods: We use high-angular-resolution images obtained with SPHERE/ZIMPOL to study R Dor and its inner envelope in a novel way. We present observations in filters V, cntHα, and cnt820 and investigate the surface brightness distribution of the star and of the polarised light produced in the inner envelope. Thanks to second-epoch observations in cntHα, we are able to see variability on the stellar photosphere. We study the polarised-light data using a continuum-radiative-transfer code that accounts for direction-dependent scattering of photons off dust grains. Results: We find that in the first epoch the surface brightness of R Dor is asymmetric in V and cntHα, the filters where molecular opacity is stronger, while in cnt820 the surface brightness is closer to being axisymmetric. The second-epoch observations in cntHα show that the morphology of R Dor has changed completely in a timespan of 48 days to a more axisymmetric and compact configuration. This variable morphology is probably linked to changes in the opacity provided by TiO molecules in the extended atmosphere. The observations show polarised light coming from a region around the central star. The inner radius of the region from where polarised light is seen varies only by a small amount with azimuth. The value of the polarised intensity, however, varies by between a factor of 2.3 and 3.7 with

  2. The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice

    PubMed Central

    Martins-Olivera, Bruno Tadeu; Theodoro-Júnior, Osmar Aparecido; Oliva, Leandro Vilela; Neto dos Santos Nunes, Natalia; Olivo, Clarice Rosa; Vilela de Brito, Marlon; Prado, Carla Máximo; Leick, Edna Aparecida; Martins, Mílton de Arruda

    2016-01-01

    Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment. PMID:27528793

  3. A Back-To-Back Barrier-N-N+ (bbBNN)Diode Tripler at 200 GHz

    NASA Technical Reports Server (NTRS)

    Choudhury, Debabani; Raisanen, Anti V.; Smith, R. Peter; Frerking, Margaret A.

    1993-01-01

    This paper describes the performance of planar back-to-back Barrier-N-N+ (bbBNN) devices for mm and submm wave multiplier applications. A technique has been developed for characterizing planar bbBNN devices with Vector Network Analyzer, which gives both the series resistance and voltage dependent capacitance of the device.

  4. De Novo whole genome sequence of Xylella fastidiosa subsp. multiplex strain BB01 from blueberry in Georgia, USA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study reports a de novo assembled draft genome sequence of Xylella fastidiosa subsp. multiplex strain BB01 causing blueberry bacterial leaf scorch in Georgia, USA. The BB01 genome is 2,517,579 bp with a G+C content of 51.8% and 2,943 open reading frames (ORFs) and 48 RNA genes....

  5. Purification and Characterization of Conjugated Bile Salt Hydrolase from Bifidobacterium longum BB536

    PubMed Central

    Grill, J.; Schneider, F.; Crociani, J.; Ballongue, J.

    1995-01-01

    Bifidobacterium species deconjugate taurocholic, taurodeoxycholic, taurochenodeoxycholic, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids. The enzyme level increases in the growth phase. No increase in activity is observed for the cytoplasmic enzyme after addition of conjugated bile acids to a stationary-phase culture. Conjugated bile salt hydrolase (BSH) was purified from Bifidobacterium longum BB536. Its apparent molecular mass in denaturing polyacrylamide gel electrophoresis was ca. 40,000 Da. The intact enzyme had a relative molecular weight of ca. 250,000 as determined by gel filtration chromatography, suggesting that the native BSH of B. longum is probably a hexamer. The purified enzyme is active towards both glycine and taurine conjugates of cholate, deoxycholate, and chenodeoxycholate. The pH optimum is in the range of 5.5 to 6.5. A loss of BSH activity is observed after incubation at temperatures higher than 42(deg)C; at 60(deg)C, 50% of the BSH activity is lost. The importance of free sulfhydryl groups at the enzyme active center is suggested. For B. longum BB536, no significant difference in the initial rate of deconjugation and enzymatic efficiency appears between bile salts. The enzymatic efficiency is higher for B. longum BB536 than for other genera. In this paper, a new method which permits a display of BSH activity directly on polyacrylamide gels is described; this method confirms the molecular weight obtained for B. longum BB536 BSH. PMID:16535071

  6. 4-1BB protects dendritic cells from prostate cancer-induced apoptosis.

    PubMed

    Youlin, Kuang; Jianwei, Zhang; Xin, Gou; Li, Zhang; Xiaodong, Weng; Xiuheng, Liu; Hengchen, Zhu; Zhiyuan, Chen

    2013-04-01

    It has been shown that human prostate cancer (PCa) cells induced apoptotic death of the most potent antigen-presenting cells, dendritic cells (DCs), which are responsible for the induction of specific antitumor immune responses. Here, we investigated the function of 4-1BB on protecting DCs from prostate cancer-induced apoptosis with an agonistic mAb to 4-1BB. RM-1 cells and DCs were co-incubated for 48 h and DC apoptosis was assessed by Annexin Vassay. TNF-α and IL-12 production were assessed by enzyme-linked immunosorbent assay (ELISA) and Bcl-2 and Bcl-xL on DCs were analyzed by Western blot. We have shown that co-incubation of RM-1 cells with DCs is accompanied by an increased level of DCs apoptosis. Triggering 4-1BB on DCs resulted in increased resistance of DCs to RM-1 cells-induced apoptosis, which was owing to the up-regulated expression of Bcl-2 and Bcl-xL, and increased secretion of TNF-αand IL-12. These results demonstrate that triggering 4-1BB on DCs could increased resistance of DCs to PCa-induced apoptosis.

  7. Hypercostimulation through 4-1BB distorts homeostasis of immune cells.

    PubMed

    Lee, Seung-Woo; Salek-Ardakani, Shahram; Mittler, Robert S; Croft, Michael

    2009-06-01

    The deleterious side effects associated with a recent clinical trial with anti-CD28 superagonist Abs have questioned the use of reagents to costimulatory molecules in human therapy. We now show that sustained signaling from an agonist Ab to 4-1BB, a member of the TNFR superfamily, results in detrimental effects on immune cell homeostasis. Repeated anti-4-1BB treatment during the reconstitution of hematopoietic cells in irradiated mice engrafted with bone marrow, or in mice infected with vaccinia virus, induced abnormal apoptosis of premature and immature B cells in the bone marrow, and led to peripheral B cell depletion. Inhibition of B cell development was indirect and due to costimulation of CD8 T cells and dependent on IFN-gamma. Moreover, anti-4-1BB also suppressed the development of NK and NKT cells, but in this case independently of T cells and IFN-gamma. The altered NK cell homeostasis resulted from activation-induced cell death triggered by anti-4-1BB. These results show that hypercostimulation elicits strong T cell immunity, but it can simultaneously distort immune homeostasis, suggesting that careful attention to activity, dose, and periodicity of treatment will be needed in any immunotherapeutic strategy with agonist Abs to costimulatory molecules.

  8. A role for platelet-derived growth factor-BB in rat postpneumonectomy compensatory lung growth.

    PubMed

    Yuan, Shizeng; Hannam, Vicky; Belcastro, Rosetta; Cartel, Nicholas; Cabacungan, Judy; Wang, Jinxia; Diambomba, Yenge; Johnstone, Leslie; Post, Martin; Tanswell, A Keith

    2002-07-01

    Unilateral pneumonectomy leads to compensatory growth in the residual lung, the mediators of which are largely unknown. We hypothesized, based on its other known roles in lung cell growth, that platelet-derived growth factor (PDGF)-BB would be an essential mediator of postpneumonectomy compensatory lung growth. Left-sided pneumonectomies were performed on 21-d-old rats, for comparison with sham-operated or unoperated control animals. Body weights were not different between groups. Right lung weights and DNA content were significantly increased (p < 0.05), compared with controls, by 10 d after pneumonectomy. The rate of DNA synthesis was maximal on d 5 postpneumonectomy. Total right lung PDGF-B mRNA and PDGF-BB protein increased after pneumonectomy, but were apparently tightly regulated, relative to total right lung beta-actin mRNA and protein content, respectively. However, PDGF-BB expression after pneumonectomy was apparently not purely constitutive, in that daily i.p. injections of a truncated soluble PDGF beta-receptor both reduced activation of the native PDGF beta-receptor, and attenuated increased lung DNA synthesis on d 3 after pneumonectomy. These findings are consistent with a critical role for PDGF-BB in postpneumonectomy lung growth.

  9. Basic FGF and PDGF-BB synergistically stimulate hyaluronan and IL-6 production by orbital fibroblasts.

    PubMed

    Virakul, Sita; Heutz, Judith W; Dalm, Virgil A S H; Peeters, Robin P; Paridaens, Dion; van den Bosch, Willem A; Hirankarn, Nattiya; van Hagen, P Martin; Dik, Willem A

    2016-09-15

    Orbital fibroblast activation is a central pathologic feature of Graves' Ophthalmopathy (GO). Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) have been proposed to contribute to GO, but their effects on orbital fibroblasts are largely unknown. We found that bFGF stimulated proliferation and hyaluronan production, but not IL-6 production by orbital fibroblasts, while VEGF hardly affected orbital fibroblast activity. Remarkably, co-stimulation of orbital fibroblasts with bFGF and PDGF-BB synergistically enhanced IL-6 and hyaluronan production and displayed an additive effect on proliferation compared to either bFGF or PDGF-BB stimulation. Nintedanib, a FGF- and PDGF-receptor targeting drug, more efficiently blocked bFGF + PDGF-BB-induced IL-6 and hyaluronan production than dasatinib that only targets PDGF-receptor. In conclusion, bFGF may contribute to orbital inflammation and tissue remodeling in GO, especially through synergistic interaction with PDGF-BB. Multi-target therapy directed at the bFGF and PDGF pathways may potentially be of interest for the treatment of GO.

  10. Kapton wire arc track testing: Per test procedure KWATT BB01

    NASA Technical Reports Server (NTRS)

    1990-01-01

    The results are presented of Kapton wire arc tracking tests performed at NASA-Johnson in 1990 using test procedure KWATT BB01. The tests were performed to gather data for use in assessing the safety of the Space Shuttle Orbiter wiring which is predominantly Kapton insulated.

  11. High temperature blackbody BB2000/40 for calibration of radiation thermometers and thermocouple

    SciTech Connect

    Ogarev, S. A.; Khlevnoy, B. B.; Samoylov, M. L.; Puzanov, A. V.

    2013-09-11

    The cavity-type high temperature blackbody (HTBB) models of BB3200/3500 series are the most spread among metrological institutes worldwide as sources for radiometry and radiation thermometry, due to their ultra high working temperatures, high emissivity and stability. The materials of radiating cavities are graphite, pyrolytic graphite (PG) and their combination. The paper describes BB2000/40 blackbody with graphite-tube cavity that was developed for calibration of radiation thermometers at SCEI (Singapore). The peculiarity of BB2000/40 is a possibility to use it, besides calibration of pyrometers, as an instrument for thermocouples calibration. Operating within the temperature range from 900 °C to 2000 °C, the blackbody has a wide cavity opening of 40 mm. Emissivity of the cavity, with PG heater rings replaced partly by graphite elements, was estimated as 0.998 ± 0.0015 in the spectral range from 350 nm to 2000 nm. The uniformity along the cavity axis, accounting for 10 °C, was measured using a B-type thermocouple at 1500 °C. The BB2000/40, if necessary, can be easily modified, by replacing the graphite radiator with a set of PG rings, to be able to reach temperatures as high as 3200 °C. The HTBB utilizes an optical feedback system which allows temperature stabilization within 0.1 °C. This rear-view feedback allows the whole HTBB aperture to be used for measurements.

  12. Section BB East Elevation Existing Condition in 2009 and Pre2004 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Section B-B East Elevation Existing Condition in 2009 and Pre-2004 Fire, Cast-Iron End Thrust Block, Cast-Iron Thrust Block, Cast-Iron Lateral Shoe - Boston & Maine Railroad, Berlin Branch Bridge #148.81, Formerly spanning Moose Brook at former Boston & Maine Railroad, Gorham, Coos County, NH

  13. Tissue engineering with recombinant human platelet-derived growth factor BB for implant site development.

    PubMed

    Nevins, Marc L; Reynolds, Mark A

    2011-03-01

    Currently, PDGF-BB is FDA-approved for periodontal regeneration as part of a dental bone-filling device only. Although this device uses beta-TCP as the scaffold carrier, there has been considerable clinical interest in combining this growth factor with other bone replacement grafts, particularly bone allografts. This article reports on clinical experiences using rhPDGF-BB with bone allografts for implant site development. After careful evaluation of clinical parameters and consideration of current and emerging evidence, the off-label use of rhPDGF-BB was determined in the following case reports to be consistent with good clinical practice and in the patient's best interest. Clinical, radiographic, and histologic observations from the following selected cases are presented to illustrate treatment outcomes achieved using this combination strategy: ridge preservation for extraction sockets with alveolar wall defects; ridge preservation for extraction sockets minimally invasive techniques; lateral ridge augmentation; and sinus augmentation. All of the cases presented and reviewed were surgically managed using 0.5 ml of 0.3 mg/ml of rhPDGF delivered using a particulate bone allograft (FDBA or DFDBA) as a scaffold. Controlled clinical trials are necessary to establish the relative effectiveness of rhPDGF-BB combined with different mammalian scaffolds for alveolar augmentation.

  14. Measurement Properties of the Smartphone-Based B-B Score in Current Shoulder Pathologies

    PubMed Central

    Pichonnaz, Claude; Duc, Cyntia; Gleeson, Nigel; Ancey, Céline; Jaccard, Hervé; Lécureux, Estelle; Farron, Alain; Jolles, Brigitte M.; Aminian, Kamiar

    2015-01-01

    This study is aimed at the determination of the measurement properties of the shoulder function B-B Score measured with a smartphone. This score measures the symmetry between sides of a power-related metric for two selected movements, with 100% representing perfect symmetry. Twenty healthy participants, 20 patients with rotator cuff conditions, 23 with fractures, 22 with capsulitis, and 23 with shoulder instabilities were measured twice across a six-month interval using the B-B Score and shoulder function questionnaires. The discriminative power, responsiveness, diagnostic power, concurrent validity, minimal detectable change (MDC), minimal clinically important improvement (MCII), and patient acceptable symptom state (PASS) were evaluated. Significant differences with the control group and significant baseline—six-month differences were found for the rotator cuff condition, fracture, and capsulitis patient groups. The B-B Score was responsive and demonstrated excellent diagnostic power, except for shoulder instability. The correlations with clinical scores were generally moderate to high, but lower for instability. The MDC was 18.1%, the MCII was 25.2%, and the PASS was 77.6. No floor effect was observed. The B-B Score demonstrated excellent measurement properties in populations with rotator cuff conditions, proximal humerus fractures, and capsulitis, and can thus be used as a routine test to evaluate those patients. PMID:26506355

  15. Measurement properties of the smartphone-based B-B Score in current shoulder pathologies.

    PubMed

    Pichonnaz, Claude; Duc, Cyntia; Gleeson, Nigel; Ancey, Céline; Jaccard, Hervé; Lécureux, Estelle; Farron, Alain; Jolles, Brigitte M; Aminian, Kamiar

    2015-10-22

    This study is aimed at the determination of the measurement properties of the shoulder function B-B Score measured with a smartphone. This score measures the symmetry between sides of a power-related metric for two selected movements, with 100% representing perfect symmetry. Twenty healthy participants, 20 patients with rotator cuff conditions, 23 with fractures, 22 with capsulitis, and 23 with shoulder instabilities were measured twice across a six-month interval using the B-B Score and shoulder function questionnaires. The discriminative power, responsiveness, diagnostic power, concurrent validity, minimal detectable change (MDC), minimal clinically important improvement (MCII), and patient acceptable symptom state (PASS) were evaluated. Significant differences with the control group and significant baseline-six-month differences were found for the rotator cuff condition, fracture, and capsulitis patient groups. The B-B Score was responsive and demonstrated excellent diagnostic power, except for shoulder instability. The correlations with clinical scores were generally moderate to high, but lower for instability. The MDC was 18.1%, the MCII was 25.2%, and the PASS was 77.6. No floor effect was observed. The B-B Score demonstrated excellent measurement properties in populations with rotator cuff conditions, proximal humerus fractures, and capsulitis, and can thus be used as a routine test to evaluate those patients.

  16. Treatment with GAD65 or BSA does not protect against diabetes in BB rats.

    PubMed

    Petersen, J S; Mackay, P; Plesner, A; Karlsen, A; Gotfredsen, C; Verland, S; Michelsen, B; Dyrberg, T

    1997-01-01

    The M(r) 65,000 isoform of glutamic acid decarboxylase (GAD65) has been implicated as the initiating islet cell antigen in the pathogenesis of diabetes, primarily based on studies in non-obese diabetic (NOD) mice. To test the role of this islet cell autoantigen in the pathogenesis of spontaneously occurring diabetes in another animal model, purified recombinant human islet GAD65 was injected i.v. at 200 micrograms/animal into 18-day-old diabetes-prone BB rats. For controls, bovine serum albumin (BSA), which has also been implicated in the pathogenesis of diabetes, or buffer alone was injected into age matched BB rats. At 210 days of age there were no differences in diabetes incidence in the 3 groups, i.e. 73% (11 of 15) in the GAD65-treated, 81% (13 of 16) in the BSA-treated and 65% (11 of 17) in the buffer-treated animals, or in the median age at onset of disease, i.e. 79 days (range 65-111), 87 days (range 60-107) and 86 days (range 74-109), respectively. The lack of protection against diabetes following GAD65 treatment could hypothetically be explained by no or by an aberrant expression of GAD in BB-rat islet cells. However, immunohistochemistry of pancreata and immunoblotting analysis of isolated islets showed that the expression of GAD65 and GAD67 was similar in BB and Lewis rats. In conclusion, these data indicate that neither GAD65 nor BSA autoimmunity is important for the development of diabetes in BB rats, in contrast to the situation in NOD mice, and further emphasizes that extrapolation from only one animal model to autoimmune diabetes in general may not be appropriate.

  17. Myofibroblast-derived PDGF-BB Promotes Hedgehog Survival Signaling in Cholangiocarcinoma Cells

    PubMed Central

    Fingas, C D; Bronk, S F; Werneburg, N W; Mott, J L; Guicciardi, M E; Cazanave, S C; Mertens, J C; Sirica, A E; Gores, G J

    2011-01-01

    Cholangiocarcinoma (CCA) cells paradoxically express the death ligand TRAIL, and, therefore, are dependent upon potent survival signals to circumvent TRAIL cytotoxicity. CCAs are also highly desmoplastic cancers with a tumor microenvironment rich in myofibroblasts (MFBs). Herein, we examine a role for MFB-derived CCA survival signals. We employed human KMCH-1, KMBC, HuCCT-1, TFK-1, and Mz-ChA-1 CCA cells as well as human primary hepatic stellate and myofibroblastic LX-2 cells for these studies. In vivo experiments were conducted using a syngeneic rat orthotopic CCA model. Co-culturing CCA cells with myofibroblastic human primary HSCs or LX-2 cells significantly decreased TRAIL-induced apoptosis in CCA cells, a cytoprotective effect abrogated by neutralizing PDGF-BB-antiserum. Cytoprotection by PDGF-BB was dependent upon Hedgehog (Hh) signaling as it was abolished by the smoothened (the transducer of Hh signaling) inhibitor cyclopamine. PDGF-BB induced PKA-dependent trafficking of smoothened to the plasma membrane resulting in GLI2 nuclear translocation and activation of a consensus GLI reporter gene-based luciferase assay. A genome-wide mRNA expression analysis identified 67 target genes to be commonly up- (50 genes) or downregulated (17 genes) by both SHH and PDGF-BB in a cyclopamine-dependent manner in CCA cells. Finally, in a rodent CCA in vivo-model, cyclopamine administration increased apoptosis in CCA cells resulting in tumor suppression. Conclusions Myofibroblast-derived PDGF-BB protects CCA cells from TRAIL cytotoxicity by a Hh signaling-dependent process. These results have therapeutical implications for the treatment of human cholangiocarcinoma. PMID:22038837

  18. Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derived Growth Factor-BB in Pichia pastoris.

    PubMed

    Dai, Mengmeng; Yu, Changming; Fang, Ting; Fu, Ling; Wang, Jing; Zhang, Jun; Ren, Jun; Xu, Junjie; Zhang, Xiaopeng; Chen, Wei

    2015-01-01

    Yeast Pichia pastoris is a widely used system for heterologous protein expression. However, post-translational modifications, especially glycosylation, usually impede pharmaceutical application of recombinant proteins because of unexpected alterations in protein structure and function. The aim of this study was to identify glycosylation sites on recombinant human platelet-derived growth factor-BB (rhPDGF-BB) secreted by P. pastoris, and investigate possible effects of O-linked glycans on PDGF-BB functional activity. PDGF-BB secreted by P. pastoris is very heterogeneous and contains multiple isoforms. We demonstrated that PDGF-BB was O-glycosylated during the secretion process and detected putative O-glycosylation sites using glycosylation staining and immunoblotting. By site-directed mutagenesis and high-resolution LC/MS analysis, we, for the first time, identified two threonine residues at the C-terminus as the major O-glycosylation sites on rhPDGF-BB produced in P. pastoris. Although O-glycosylation resulted in heterogeneous protein expression, the removal of glycosylation sites did not affect rhPDGF-BB mitogenic activity. In addition, the unglycosylated PDGF-BBΔGly mutant exhibited the immunogenicity comparable to that of the wild-type form. Furthermore, antiserum against PDGF-BBΔGly also recognized glycosylated PDGF-BB, indicating that protein immunogenicity was unaltered by glycosylation. These findings elucidate the effect of glycosylation on PDGF-BB structure and biological activity, and can potentially contribute to the design and production of homogeneously expressed unglycosylated or human-type glycosylated PDGF-BB in P. pastoris for pharmaceutical applications.

  19. Induction of cell migration in vitro by an electrospun PDGF-BB/PLGA/PEG-PLA nanofibrous scaffold.

    PubMed

    Zhao, Xia; Hadjiargyrou, Michael

    2011-12-01

    Electrospinning is a versatile technique used to fabricate potential tissue engineering scaffolds with a structure similar to the native extracellular matrix (ECM). In this study, Platelet-Derived Growth Factor (PDGF)-BB with BSA as a carrier protein was incorporated into an electrospun PLGA/PEG-PLA composite scaffold for induction of cell migration, an early process necessary for tissue regeneration and wound healing. Incorporating PDGF-BB into the fibers did not change the overall morphology of the scaffold, with the exception of a slight increase (approximately 12%) in the number of fibers with diameters ranging from 1-100 nm. Following a strong burst of release during the initial 24 hours, approximately 20% of the total incorporated PDGF-BB was released from the scaffold over 5 days, as determined by ELISA. The presence of the released PDGF-BB was also confirmed via SDS-PAGE. Using an in vitro agarose-cell migration assay with MC3T3 pre-osteoblastic cells, the preserved bioactivity of the released PDGF-BB was demonstrated via its ability to stimulate robust cell migration, equivalent to that of pure unincorporated (control) PDGF-BB. Overall, this study demonstrates that it is feasible to incorporate and deliver bioactive PDGF-BB via an electrospun scaffold for potential tissue repair applications, especially as a potent inducer of cell migration.

  20. Greenhouse-Selected Resistance to Cry3Bb1-Producing Corn in Three Western Corn Rootworm Populations

    PubMed Central

    Meihls, Lisa N.; Higdon, Matthew L.; Ellersieck, Mark R.; Tabashnik, Bruce E.; Hibbard, Bruce E.

    2012-01-01

    Transgenic corn producing the Bacillus thuringiensis (Bt) toxin Cry3Bb1 has been useful for controlling western corn rootworm, Diabrotica virgifera virgifera LeConte, one of the most economically important crop pests in the United States. However, rapid evolution of resistance by this beetle to Bt corn producing Cry3Bb1 has been reported previously from the laboratory, greenhouse, and field. Here we selected in the greenhouse for resistance to Cry3Bb1 corn in three colonies of WCR derived from Kansas, Minnesota, and Wisconsin, respectively. Three generations of rearing on Cry3Bb1 corn significantly increased larval survival on Cry3Bb1 corn, resulting in similar survival in the greenhouse for selected colonies on Cry3Bb1 corn and isoline corn that does not produce Bt toxin. After four to seven generations of rearing on Cry3Bb1 corn, survival in the field on Cry3Bb1 corn relative to isoline corn more than doubled for selected colonies (72%) compared with control colonies (33%). For both selected and control colonies, survival in the field was significantly lower on Cry3Bb1 corn than on isoline corn. On isoline corn, most fitness components were similar for selected colonies and control colonies. However, fecundity was significantly lower for selected colonies than control colonies, indicating a fitness cost associated with resistance. The rapid evolution of resistance by western corn rootworm to Bt corn reported here and previously underlines the importance of effective resistance management for this pest. PMID:23284656

  1. Fast and stable evaluation of box-splines via the BB-form

    NASA Astrophysics Data System (ADS)

    Kim, Minho; Peters, Jörg

    2009-04-01

    To repeatedly evaluate linear combinations of box-splines in a fast and stable way, in particular along knot planes, the box-spline is converted to and tabulated as piecewise polynomial in BB-form (Bernstein-Bézier-form). We show that the BB-coefficients can be derived and stored as integers plus a rational scale factor and derive a hash table for efficiently accessing the polynomial pieces. This pre-processing, the resulting evaluation algorithm and use in a widely-used ray-tracing package are illustrated for splines based on two trivariate box-splines: the seven-directional box-spline on the Cartesian lattice and the six-directional box-spline on the face-centered cubic lattice.

  2. Transition-metal-free B-B and B-interelement reactions with organic molecules.

    PubMed

    Cuenca, Ana B; Shishido, Ryosuke; Ito, Hajime; Fernández, Elena

    2017-01-23

    This review is a guided tour along the activation modes and reactivity of B-B, B-Si, B-N, B-S, B-Se and B-P reagents, in the absence of any transition metal complex. Here are disclosed the general concepts related to the homolytic and heterolytic cleavage of B-B and B-interelement bonds, as well as the generation of new C-B and C-interelement bonds, in a selective way. The greener consequences of these novel routes facilitate the gram scale preparations of target functionalised organic compounds. Intrinsic data about the suggested mechanisms and spectroscopic evidence that supports the innovative theories are provided along the review. Since this is a stimulating area of work that has emerged within the last decade, this overview serves as the basis to understand the new trends and hopefully to generate inspiration for future discoveries in the field.

  3. "13 BB star" - broadband seismic array at the edge of East European Craton in Poland

    NASA Astrophysics Data System (ADS)

    Polkowski, Marcin; Grad, Marek; Wilde-Piórko, Monika; Suchcicki, Jerzy; Arant, Tadeusz

    2014-05-01

    "13 BB star" is a dense array of 13 Reftek 151-120 broadband seismometers located in unpopulated forests in northern Poland - just on the edge of East European Craton. The shape of array was designed as a "star" with one central station and 12 station located on two circles (radius of about 30 and 60 km) around it. This geometry allows us to study seismic waves (in particular surface waves) incoming from all azimuths. Our "13 BB star" array stations are self powered (solar panels and batteries), equipped with local storage system and on-line transmission of seismic and auxiliary data using 2G and 3G cellular network. We also developed on-line application for monitoring array status (transmission, connections, power, temperature, etc.) and on-line data visualization. Main advantages of our array are: 1) 120s broadband seismometers recording with 100Hz sampling, 2) density of array - distances between neighboring stations does not exceed 30 km, 3) station placement in underground wells, 4) stations located in forests far (usually 2-3 km) from industries, roads, villages and other human activity. "13 BB star" started operating in July 2013 and since that time recorded several local, regional, and teleseismic events proving good array functionality. The scientific aim of the "13 BB star" project is development of a quantitative model of the lithosphere-asthenosphere-structure in the marginal zone of the East European Craton in northern Poland. New acquired data will be analyzed using integrated seismic methods, which will yield images of lithosphere-asthenosphere system hitherto unknown for this area. The knowledge of detailed structure of the cratonic lithosphere-asthenosphere system is crucial for the better understanding of the regional, as well as global mantle dynamics and evolution of the Earth's interior. This work was supported by NCN-grant DEC 2011/02/A/ST10/00284.

  4. Aptamer-functionalized silver nanoparticles for scanometric detection of platelet-derived growth factor-BB.

    PubMed

    Hu, Hongting; Li, Hui; Zhao, Yaju; Dong, Shiyu; Li, Wei; Qiang, Weibing; Xu, Danke

    2014-02-17

    In this work, we reported a scanometric assay system based on the aptamer-functionalized silver nanoparticles (apt-AgNPs) for detection of platelet-derived growth factor-BB (PDGF-BB) protein. The aptamer and ssDNA were bound with silver nanoparticles by self-assembly of sulfhydryl group at 5' end to form the apt-AgNPs probe. The apt-AgNPs probe can catalyze the reduction of metallic ions in color agent to generate metal deposition that can be captured both by human eyes and a flatbed scanner. Two different color agents, silver enhancer solution and color agent 1 (10 mM HAuCl4+2 mM hydroquinone) were used to develop silver and gold shell on the surface of AgNPs separately. The results demonstrated that the formation of Ag core-Au shell structure had some advantages especially in the low concentrations. The apt-AgNPs probe coupled with color agent 1 showed remarkable superiority in both sensitivity and detection limit compared to the apt-AuNPs system. The apt-AgNPs system also produced a wider linear range from 1.56 ng mL(-1) to 100 ng mL(-1) for PDGF-BB with the detection limit lower than 1.56 ng mL(-1). The present strategy was applied to the determination of PDGF-BB in 10% serum, and the results showed that it had good specificity in complex biological media.

  5. Iptakalim inhibits PDGF-BB-induced human airway smooth muscle cells proliferation and migration.

    PubMed

    Liu, Wenrui; Kong, Hui; Zeng, Xiaoning; Wang, Jingjing; Wang, Zailiang; Yan, Xiaopei; Wang, Yanli; Xie, Weiping; Wang, Hong

    2015-08-15

    Chronic airway diseases are characterized by airway remodeling which is attributed partly to the proliferation and migration of airway smooth muscle cells (ASMCs). ATP-sensitive potassium (KATP) channels have been identified in ASMCs. Mount evidence has suggested that KATP channel openers can reduce airway hyperresponsiveness and alleviate airway remodeling. Opening K(+) channels triggers K(+) efflux, which leading to membrane hyperpolarization, preventing Ca(2+)entry through closing voltage-operated Ca(2+) channels. Intracellular Ca(2+) is the most important regulator of muscle contraction, cell proliferation and migration. K(+) efflux decreases Ca(2+) influx, which consequently influences ASMCs proliferation and migration. As a KATP channel opener, iptakalim (Ipt) has been reported to restrain the proliferation of pulmonary arterial smooth muscle cells (PASMCs) involved in vascular remodeling, while little is known about its impact on ASMCs. The present study was designed to investigate the effects of Ipt on human ASMCs and the mechanisms underlying. Results obtained from cell counting kit-8 (CCK-8), flow cytometry and 5-ethynyl-2'-deoxyuridine (EdU) incorporation showed that Ipt significantly inhibited platelet-derived growth factor (PDGF)-BB-induced ASMCs proliferation. ASMCs migration induced by PDGF-BB was also suppressed by Ipt in transwell migration and scratch assay. Besides, the phosphorylation of Ca(2+)/calmodulin-dependent kinase II (CaMKII), extracellular regulated protein kinases 1/2 (ERK1/2), protein kinase B (Akt), and cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) were as well alleviated by Ipt administration. Furthermore, we found that the inhibition of Ipt on the PDGF-BB-induced proliferation and migration in human ASMCs was blocked by glibenclamide (Gli), a selective KATP channel antagonist. These findings provide a strong evidence to support that Ipt antagonize the proliferating and migrating effects of PDGF-BB on

  6. Full characterization of holographic reflection gratings recorded on BB640 emulsions.

    PubMed

    Ulibarrena, Manuel; Carretero, Luis; Madrigal, Roque; Blaya, Salvador; Fimia, Antonio

    2004-07-20

    A complete characterization of unslanted holographic reflection gratings with high diffraction efficiency recorded on ultrafine grain emulsion BB640 has been achieved. By use of a wavelength-dependent absorption coefficient, the diffraction efficiency, the replay wavelength, and the spectral bandwidth of each recording has been obtained. Corresponding index modulation, absorption, and effective thickness have been obtained with high accuracy by fitting the experimental data with Kogelnik's theory.

  7. Implications of final L3 measurement of {sigma}{sub tot}({gamma}{gamma}{yields}bb)

    SciTech Connect

    Chyla, Jiri

    2006-02-01

    The excess of data on the total cross section of bb production in {gamma}{gamma} collisions over QCD predictions, observed by L3, OPAL and DELPHI Collaborations at LEP2, has so far defied explanation. The recent final analysis of L3 data has brought important new information concerning the dependence of the observed excess on the {gamma}{gamma} collisions energy W{sub {gamma}}{sub {gamma}}. The implications of this dependence are discussed.

  8. Key rate for calibration robust entanglement based BB84 quantum key distribution protocol

    SciTech Connect

    Gittsovich, O.; Moroder, T.

    2014-12-04

    We apply the approach of verifying entanglement, which is based on the sole knowledge of the dimension of the underlying physical system to the entanglement based version of the BB84 quantum key distribution protocol. We show that the familiar one-way key rate formula holds already if one assumes the assumption that one of the parties is measuring a qubit and no further assumptions about the measurement are needed.

  9. Multimodal Broadband Vibrational Sum Frequency Generation (MM-BB-V-SFG) Spectrometer and Microscope.

    PubMed

    Lee, Christopher M; Kafle, Kabindra; Huang, Shixin; Kim, Seong H

    2016-01-14

    A broadband sum frequency generation (BB-SFG) spectrometer with multimodal (MM) capabilities was constructed, which could be routinely reconfigured for tabletop experiments in reflection, transmission, and total internal reflection (TIR) geometries, as well as microscopic imaging. The system was constructed using a Ti:sapphire amplifier (800 nm, pulse width = 85 fs, repetition rate = 2 kHz), an optical parameter amplification (OPA) system for production of broadband IR pulses tunable between 1000 and 4000 cm(-1), and two Fabry-Pérot etalons arranged in series for production of narrowband 800 nm pulses. The key feature allowing the MM operation was the nearly collinear alignment of the visible (fixed, 800 nm) and infrared (tunable, 1000-4000 cm(-1)) pulses which were spatially separated. Physical insights discussed in this paper include the comparison of spectral bandwidth produced with 40 and 85 fs pump beams, the improvement of spectral resolution using etalons, the SFG probe volume in bulk analysis, the normalization of SFG signals, the stitching of multiple spectral segments, and the operation in different modes for air/liquid and adsorbate/solid interfaces, bulk samples, as well as spectral imaging combined with principle component analysis (PCA). The SFG spectral features obtained with the MM-BB-SFG system were compared with those obtained with picosecond-scanning-SFG system and high-resolution BB-SFG system (HR-BB-SFG) for dimethyl sulfoxide, α-pinene, and various samples containing cellulose (purified commercial products, Cladophora cell wall, cotton and flax fibers, and onion epidermis cell wall).

  10. Structure-activity relationship of (-) mammea A/BB derivatives against Leishmania amazonensis.

    PubMed

    Brenzan, Mislaine Adriana; Nakamura, Celso Vataru; Dias Filho, Benedito Prado; Ueda-Nakamura, Tânia; Young, Maria Claudia M; Côrrea, Arlene Gonçalves; Alvim, Joel; dos Santos, Adriana Oliveira; Cortez, Diógenes Aparício Garcia

    2008-11-01

    To study the structure-activity relationship of coumarin (-) mammea A/BB isolated from the CH(2)Cl(2) extract of Calophyllum brasiliense leaves, we evaluated the antileishmanial activity of natural, synthetic and derivatives of this coumarin, against promastigote and intracellular amastigote forms of Leishmania amazonensis, and their cytotoxicity to J774G8 murine macrophages. The derivatives were obtained by hydrogenation and methoxylation reactions. The compound structures were elucidated on the basis of spectroscopic data. The compounds 5,7-dihydroxy-8-(2-methylbutanoyl)-6-(3-methylbutyl)-4-phenyl-chroman-2-one (3), 7-hydroxy-5-methoxy-8-(2-methylbutanoyl)-6-(3-methylbut-2-en-1-yl)-4-phenylcoumarin (4) and 5,7-dimethoxy-8-(1-methoxy-2-methylbutyl)-6-(3-methylbut-2-en-1-yl)-4 phenylcoumarin (6) were more biologically active than the compound (-) mammea A/BB (1) (7.4 microM), with IC(50) values from 0.9, 2.4 and 1.9 microM respectively; compound (3) displayed the highest activity. The compounds mammea B/BB (2), 5,7-dimethoxy-8-(2-methylbutanoyl)-6-(3-methylbut-2-en-1-yl)-4-phenylcoumarin (5) and 5,7-dihydroxy-4-phenylcoumarin (7) were less active than (-) mammea A/BB (1), with IC(50) of 30.1, 15.1 and 60.2 microM respectively; compound (7) showed the lowest antileishmanial activity of all. Compounds (1), (3), (4) and (6) were active not only against promastigote forms of L. amazonensis, but also against intracellular amastigote forms with IC(50) of 14.3, 0.6, 34.0 and 22.2 microM, respectively. Interestingly, compound (3) showed the most antileishmanial activity of all. This study demonstrated that several aspects of the structure were important for antileishmanial activity.

  11. Fibronectin peptides that bind PDGF-BB enhance survival of cells and tissue under stress

    PubMed Central

    Lin, Fubao; Zhu, Jia; Tonnesen, Marcia G.; Taira, Breena R.; McClain, Steve A.; Singer, Adam J.; Clark, Richard A.F.

    2013-01-01

    Stressors after injury from a multitude of factors can lead to cell death. We have identified four fibronectin (FN) peptides, two from the first FN type III repeat (FNIII1), one from the 13th FN type III repeat (FNIII13), and one from FN variable region (IIICS), that when tethered to a surface acted as platelet-derived growth factor-BB (PDGF-BB) enhancers to promote cell survival. One of the FNIII1 peptides and its smallest (14mer) bioactive form (P12) were also active in solution. Specifically, P12 bound PDGF-BB (KD = 200nM), enhanced adult human dermal fibroblast (AHDF) survival under serum starvation, oxidative or endoplasmic reticulum (ER) stressors, and limited burn injury progression in a rat hot comb model. Furthermore, P12 inhibited ER stress-induced c-Jun N-terminal kinase (JNK) activation. Although many growth factors have been found to bind FN directly or indirectly, this is the first report to identify peptide sequences of growth factor-binding sites in FN. The finding of these novel peptides further delineated how the extracellular matrix protein FN can support cell survival. Since the peptide P12 is active in either soluble form or tethered to a substrate, it will have multifactorial uses as a bioactive in tissue engineering. PMID:24126844

  12. Abrogation of apoptosis through PDGF-BB-induced sulfated glycosaminoglycan synthesis and secretion.

    PubMed

    Cartel, Nicholas J; Post, Martin

    2005-02-01

    Platelet-derived growth factor (PDGF)-BB-stimulated glycosaminoglycan (GAG) synthesis/secretion in fetal lung fibroblasts is dependent on sequential activation of the PDGF beta-receptor, phosphatidylinositol 3-kinase (PI3K), the serine/threonine kinase Akt-1,2, and the GTPase Rab3D. Because the Akt pathway has been implicated in cell survival mechanisms, we investigated whether the pathway regulating GAG synthesis/secretion was antiapoptotic. PDGF-BB treatment protected fetal lung fibroblasts against serum starvation-induced apoptosis, whereas wortmannin, an inhibitor of PI3K, abrogated this protective effect. Transfection of constitutively active Akt into fetal lung fibroblasts also safeguarded the cells from apoptosis induced by serum starvation. To determine whether the antiapoptotic response was due, at least in part, to GAGs, we treated lung fibroblasts with beta-D-xyloside as well as with topically applied GAGs, specifically those produced by fetal lung fibroblasts. beta-D-xyloside increased GAG synthesis/secretion and diminished apoptosis. Application of sulfated GAGs, chondroitin sulfate, and heparan sulfate, but not nonsulfated hyaluronan, also resulted in diminished apoptosis. Moreover, topically applied sulfated GAGs increased Bcl-associated death promoter phosphorylation and diminished caspase-3 and -7 cleavage, indicating an antiapototic response. These data are compatible with the PDGF-BB-GAG signaling pathway regulating programmed fibroblast death in the fetal lung.

  13. Depressed glucose utilization in lungs of BB wistar spontaneously diabetic rats

    SciTech Connect

    Uhal, B.D.; Moxley, M.A.; Longmore, W.J.

    1986-03-05

    Lungs of BB wistar spontaneously diabetic rats were perfused with (/sup 14/C(U))glucose in modified Krebs Ringer bicarbonate medium for 1.5 hours. Lungs from non-diabetic BB Wistar rats were perfused simultaneously and served as controls. The perfusions were terminated by rapid freezing of the tissue in liquid N/sub 2/ followed by separation of surfactant and residual lung fractions. The rates of glucose incorporation into surfactant DSPC, PG, and PE were decreased 4.7, 2.4 and 2.5-fold, respectively, in lungs of spontaneously diabetic rats when expressed as final product specific activities. The rate of glucose incorporation into residual PC was also reduced by 2.3-fold. Expressed as moles incorporated per gram wet weight of lung, incorporations into surfactant DSPC, PG and residual PC were also reduced by 4.1, 6.3 and 3.8-fold respectively. These data; (1) agree with previous studies of the lungs of streptozotocin and alloxan-diabetic rats; (2) show that the depressed glucose utilization for lipid synthesis observed previously is not due to streptozotocin or alloxan toxicity; (3) suggest that the BB Wistar rat will provide a useful model for the study of the effects of insulin-dependent diabetes on lung metabolism.

  14. Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells.

    PubMed

    Wang, Ying; Wang, Yan; Liu, Dan; Wang, Wang; Zhao, Huan; Wang, Min; Yin, Hongping

    2015-07-10

    CPS-F, a polysaccharide derived from Cordyceps sinensis, is a potential anti-inflammatory and anti-oxidative agent. We demonstrated that CPS-F not only inhibits platelet-derived growth factor BB (PDGF-BB)-induced intracellular reactive oxygen species (ROS) generation, and up-regulation of tumor necrosis factor-α (TNF-α), TNF-α receptor 1 (TNFR1), and monocyte chemotactic protein-1 (MCP-1), but also acts synergistically in combination with MAPK/ERK inhibitor U0126 and PI3K/Akt inhibitor LY294002. Additionally, up-regulation of pro-inflammatory factors was reversed by use of a combination of CPS-F and NADPH oxidase (NOX) inhibitor diphenyleneiodonium chloride (DPI) or silencing of NOX1. Furthermore, CPS-F prevents the PDGF receptor β (PDGFRβ) promoter activity induced by PDGF-BB in transfected cells and ameliorates increased levels of TNF-α, TNFR1, and MCP-1 when PDGFRβ is silenced, thereby suggesting that CPS-F possesses a bidirectional regulatory function. Our findings suggest CPS-F may exert its therapeutic effect for the treatment of glomerulonephritis related to human mesangial cells (HMCs) through the ERK1/2/Akt pathways.

  15. Inclusive b and bb¯ production with quasi-multi-Regge kinematics at the Tevatron

    NASA Astrophysics Data System (ADS)

    Kniehl, B. A.; Saleev, V. A.; Shipilova, A. V.

    2010-05-01

    We consider b-jet hadroproduction in the quasi-multi-Regge-kinematics approach based on the hypothesis of gluon and quark Reggeization in t-channel exchanges at high energies. The preliminary data on inclusive b-jet and bb¯-dijet production taken by the CDF Collaboration at the Fermilab Tevatron are well described without adjusting parameters. We find the main contribution to inclusive b-jet production to be the scattering of a Reggeized gluon and a Reggeized b quark to a b quark, which is described by the effective Reggeon-Reggeon-quark vertex. The main contribution to bb¯-pair production arises from the scattering of two Reggeized gluons to a bb¯ pair, which is described by the effective Reggeon-Reggeon-quark-quark vertex. Our analysis is based on the Kimber-Martin-Ryskin prescription for unintegrated gluon and quark distribution functions using as input the Martin-Roberts-Stirling-Thorne collinear parton distribution functions of the proton.

  16. Empigen BB: a useful detergent for solubilization and biochemical analysis of keratins.

    PubMed

    Lowthert, L A; Ku, N O; Liao, J; Coulombe, P A; Omary, M B

    1995-01-05

    Intermediate filament (IF) proteins make up some of the most insoluble proteins known, and within the IF protein family, keratins are the least soluble. We compared the efficiency of nonionic, cationic, mixed nonionic and anionic, and zwitterionic detergents in solubilizing keratins from insect cells that express recombinant human keratins and from human colonic cell lines and normal keratinocytes. The cationic detergent cetyltrimethylammonium bromide was similar to the zwitterionic detergent Empigen BB in its ability to efficiently solubilize keratins, but the latter detergent was superior in that it maintained antibody reactivity and allowed for immunoprecipitation of the keratins. Although Nonidet-P40 partially solubilizes keratins, Empigen BB solubilizes a significant amount of keratins not solubilized by Nonidet-P40. In the case of vimentin, differences in solubilization efficiency among the detergents was not as dramatic as with keratins. Our results show that Empigen BB solubilizes a significant amount of epidermal and glandular keratins while preserving antigenicity. This detergent should prove useful for carrying out biochemical and molecular studies on these proteins and may be similarly beneficial for other IF proteins.

  17. The VMC Survey. XXII. Hierarchical Star Formation in the 30 Doradus-N158–N159–N160 Star-forming Complex

    NASA Astrophysics Data System (ADS)

    Sun, Ning-Chen; de Grijs, Richard; Subramanian, Smitha; Cioni, Maria-Rosa L.; Rubele, Stefano; Bekki, Kenji; Ivanov, Valentin D.; Piatti, Andrés E.; Ripepi, Vincenzo

    2017-02-01

    We study the hierarchical stellar structures in a ∼1.5 deg2 area covering the 30 Doradus-N158–N159–N160 star-forming complex with the VISTA Survey of Magellanic Clouds. Based on the young upper main-sequence stars, we find that the surface densities cover a wide range of values, from log({{Σ }}\\cdot pc2) ≲ ‑2.0 to log({{Σ }}\\cdot pc2) ≳ 0.0. Their distributions are highly non-uniform, showing groups that frequently have subgroups inside. The sizes of the stellar groups do not exhibit characteristic values, and range continuously from several parsecs to more than 100 pc the cumulative size distribution can be well described by a single power law, with the power-law index indicating a projected fractal dimension D2 = 1.6 ± 0.3. We suggest that the phenomena revealed here support a scenario of hierarchical star formation. Comparisons with other star-forming regions and galaxies are also discussed.

  18. Broad Balmer Wings in BA Hyper/Supergiants Distorted by Diffuse Interstellar Bands: Five Examples in the 30 Doradus Region from the VLT-FLAMES Tarantula Survey

    NASA Astrophysics Data System (ADS)

    Walborn, Nolan R.; Sana, Hugues; Evans, Christopher J.; Taylor, William D.; Sabbi, Elena; Barbá, Rodolfo H.; Morrell, Nidia I.; Maíz Apellániz, Jesús; Sota, Alfredo; Dufton, Philip L.; McEvoy, Catherine M.; Clark, J. Simon; Markova, Nevena; Ulaczyk, Krzysztof

    2015-08-01

    Extremely broad emission wings at Hβ and Hα have been found in VLT-FLAMES Tarantula Survey data for five very luminous BA supergiants in or near 30 Doradus in the Large Magellanic Cloud. The profiles of both lines are extremely asymmetrical, which we have found to be caused by very broad diffuse interstellar bands (DIBs) in the longward wing of Hβ and the shortward wing of Hα. These DIBs are well known to interstellar but not to many stellar specialists, so that the asymmetries may be mistaken for intrinsic features. The broad emission wings are generally ascribed to electron scattering, although we note difficulties for that interpretation in some objects. Such profiles are known in some Galactic hyper/supergiants and are also seen in both active and quiescent Luminous Blue Variables (LBVs). No prior or current LBV activity is known in these 30 Dor stars, although a generic relationship to LBVs is not excluded; subject to further observational and theoretical investigation, it is possible that these very luminous supergiants are approaching the LBV stage for the first time. Their locations in the HRD and presumed evolutionary tracks are consistent with that possibility. The available evidence for spectroscopic variations of these objects is reviewed, while recent photometric monitoring does not reveal variability. A search for circumstellar nebulae has been conducted, with an indeterminate result for one of them.

  19. BROAD BALMER WINGS IN BA HYPER/SUPERGIANTS DISTORTED BY DIFFUSE INTERSTELLAR BANDS: FIVE EXAMPLES IN THE 30 DORADUS REGION FROM THE VLT-FLAMES TARANTULA SURVEY

    SciTech Connect

    Walborn, Nolan R.; Sana, Hugues; Sabbi, Elena E-mail: hsana@stsci.edu; and others

    2015-08-10

    Extremely broad emission wings at Hβ and Hα have been found in VLT-FLAMES Tarantula Survey data for five very luminous BA supergiants in or near 30 Doradus in the Large Magellanic Cloud. The profiles of both lines are extremely asymmetrical, which we have found to be caused by very broad diffuse interstellar bands (DIBs) in the longward wing of Hβ and the shortward wing of Hα. These DIBs are well known to interstellar but not to many stellar specialists, so that the asymmetries may be mistaken for intrinsic features. The broad emission wings are generally ascribed to electron scattering, although we note difficulties for that interpretation in some objects. Such profiles are known in some Galactic hyper/supergiants and are also seen in both active and quiescent Luminous Blue Variables (LBVs). No prior or current LBV activity is known in these 30 Dor stars, although a generic relationship to LBVs is not excluded; subject to further observational and theoretical investigation, it is possible that these very luminous supergiants are approaching the LBV stage for the first time. Their locations in the HRD and presumed evolutionary tracks are consistent with that possibility. The available evidence for spectroscopic variations of these objects is reviewed, while recent photometric monitoring does not reveal variability. A search for circumstellar nebulae has been conducted, with an indeterminate result for one of them.

  20. Assessment of fitness costs in Cry3Bb1 resistant and susceptible western corn rootworm (Coleoptera:Chrysomelidae) laboratory colonies

    EPA Science Inventory

    Maize production in the United States is dominated by plants genetically modified with transgenes from Bacillus thuringiensis (Bt). Varieties of Bt maize expressing Cry3Bb endotoxins that specifically target corn rootworms (genus Diabrotica) have proven highly efficacious. Howeve...

  1. Iptakalim inhibits PDGF-BB-induced human airway smooth muscle cells proliferation and migration

    SciTech Connect

    Liu, Wenrui; Kong, Hui; Zeng, Xiaoning; Wang, Jingjing; Wang, Zailiang; Yan, Xiaopei; Wang, Yanli; Xie, Weiping Wang, Hong

    2015-08-15

    Chronic airway diseases are characterized by airway remodeling which is attributed partly to the proliferation and migration of airway smooth muscle cells (ASMCs). ATP-sensitive potassium (K{sub ATP}) channels have been identified in ASMCs. Mount evidence has suggested that K{sub ATP} channel openers can reduce airway hyperresponsiveness and alleviate airway remodeling. Opening K{sup +} channels triggers K{sup +} efflux, which leading to membrane hyperpolarization, preventing Ca{sup 2+}entry through closing voltage-operated Ca{sup 2+} channels. Intracellular Ca{sup 2+} is the most important regulator of muscle contraction, cell proliferation and migration. K{sup +} efflux decreases Ca{sup 2+} influx, which consequently influences ASMCs proliferation and migration. As a K{sub ATP} channel opener, iptakalim (Ipt) has been reported to restrain the proliferation of pulmonary arterial smooth muscle cells (PASMCs) involved in vascular remodeling, while little is known about its impact on ASMCs. The present study was designed to investigate the effects of Ipt on human ASMCs and the mechanisms underlying. Results obtained from cell counting kit-8 (CCK-8), flow cytometry and 5-ethynyl-2′-deoxyuridine (EdU) incorporation showed that Ipt significantly inhibited platelet-derived growth factor (PDGF)-BB-induced ASMCs proliferation. ASMCs migration induced by PDGF-BB was also suppressed by Ipt in transwell migration and scratch assay. Besides, the phosphorylation of Ca{sup 2+}/calmodulin-dependent kinase II (CaMKII), extracellular regulated protein kinases 1/2 (ERK1/2), protein kinase B (Akt), and cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) were as well alleviated by Ipt administration. Furthermore, we found that the inhibition of Ipt on the PDGF-BB-induced proliferation and migration in human ASMCs was blocked by glibenclamide (Gli), a selective K{sub ATP} channel antagonist. These findings provide a strong evidence to support that Ipt

  2. Sandwich-format electrochemiluminescence assay for PDGF-BB using quantum dots-dendrimer nanocomposites as probe.

    PubMed

    Zhang, Jing-Jing; Cao, Jun-Tao; Shi, Gui-Fang; Liu, Yan-Ming; Chen, Yong-Hong; Ren, Shu-Wei

    2015-08-15

    This work describes a novel electrochemiluminescence aptasensor for highly sensitive detection of platelet-derived growth factor BB (PDGF-BB) using aptamer functionalized CdS quantum dots-polyamidoamine as probe (CdS QDs-PAMAM-Apt). CdS QDs-PAMAM nanocomposites were synthesized by one-pot synthesis in methanol. The prepared nanocomposites were linked with the NH2-aptamer 2 (Apt2) of PDGF-BB to form the CdS QDs-PAMAM-Apt2 probe by glutaraldehyde as coupling reagent. For constructing the aptasensor, MWCNTs-chitosan composites and NH2-aptamer 1 (Apt1) with the same base sequence as Apt2 were immobilized on the electrode by the self-assembled method to recognize the target protein PDGF-BB. In the presence of PDGF-BB, the structure of sandwiched format was formed between the Apt1 and the CdS QDs-PAMAM-Apt2 probe, thereby resulting in a proportional increase of ECL emission. Thanks to the efficient and stable ECL emission of CdS QDs-PAMAM dendrimer and the advantage of MWCNTs for accelerating the electron transfer, the highly sensitive detection of PDGF-BB with a detection limit of 0.13pM was achieved. The linear range is from 0.5pM to 1nM. The present protocol was applied to the analysis of PDGF-BB in human serum samples. The recoveries of PDGF-BB in human serum samples are 87.2-113% and RSD values are less than 3.6%.

  3. 1993/03 Baccalaureate and Beyond Longitudinal Study (B&B:93/03). Methodology Report. NCES 2006-166

    ERIC Educational Resources Information Center

    Wine, Jennifer S.; Cominole, Melissa B.; Wheeless, Sara; Dudley, Kristin; Franklin, Jeff

    2005-01-01

    This report describes the procedures and results of the full-scale implementation of the B&B:93/03 study. Students who earned a bachelor's degree in 1992-93 were first interviewed in 1993 and then subsequently in 1994 and 1997. This is the final follow-up interview of the B&B:93 cohort, 10 years following completion of the bachelor's…

  4. PDGF-BB-mediated activation of p42(MAPK) is independent of PDGF beta-receptor tyrosine phosphorylation.

    PubMed

    Cartel, N J; Liu, J; Wang, J; Post, M

    2001-10-01

    Herein, we investigated the activity of mitogen-activated protein kinase (MAPK), a key component of downstream signaling events, which is activated subsequent to platelet-derived growth factor (PDGF)-BB stimulation. Specifically, p42(MAPK) activity peaked 60 min after addition of PDGF-BB, declined thereafter, and was determined not to be a direct or necessary component of glycosaminoglycan (GAG) synthesis. PDGF-BB also activated MAPK kinase 2 (MAPKK2) but had no effect on MAPKK1 and Raf-1 activity. Chemical inhibition of Janus kinase, phosphatidylinositol 3-kinase, Src kinase, or tyrosine phosphorylation inhibition of the PDGF beta-receptor (PDGFR-beta) did not abrogate PDGF-BB-induced p42(MAPK) activation or its threonine or tyrosine phosphorylation. A dominant negative cytoplasmic receptor for hyaluronan-mediated motility variant 4 (RHAMMv4), a regulator of MAPKK-MAPK interaction and activation, did not inhibit PDGF-BB-induced p42(MAPK) activation nor did a construct expressing PDGFR-beta with cytoplasmic tyrosines mutated to phenylalanine. However, overexpression of a dominant negative PDGFR-beta lacking the cytoplasmic signaling domain abrogated p42(MAPK) activity. These results suggest that PDGF-BB-mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation.

  5. Differences in acid tolerance between Bifidobacterium breve BB8 and its acid-resistant derivative B. breve BB8dpH, revealed by RNA-sequencing and physiological analysis.

    PubMed

    Yang, Xu; Hang, Xiaomin; Tan, Jing; Yang, Hong

    2015-06-01

    Bifidobacteria are common inhabitants of the human gastrointestinal tract, and their application has increased dramatically in recent years due to their health-promoting effects. The ability of bifidobacteria to tolerate acidic environments is particularly important for their function as probiotics because they encounter such environments in food products and during passage through the gastrointestinal tract. In this study, we generated a derivative, Bifidobacterium breve BB8dpH, which displayed a stable, acid-resistant phenotype. To investigate the possible reasons for the higher acid tolerance of B. breve BB8dpH, as compared with its parental strain B. breve BB8, a combined transcriptome and physiological approach was used to characterize differences between the two strains. An analysis of the transcriptome by RNA-sequencing indicated that the expression of 121 genes was increased by more than 2-fold, while the expression of 146 genes was reduced more than 2-fold, in B. breve BB8dpH. Validation of the RNA-sequencing data using real-time quantitative PCR analysis demonstrated that the RNA-sequencing results were highly reliable. The comparison analysis, based on differentially expressed genes, suggested that the acid tolerance of B. breve BB8dpH was enhanced by regulating the expression of genes involved in carbohydrate transport and metabolism, energy production, synthesis of cell envelope components (peptidoglycan and exopolysaccharide), synthesis and transport of glutamate and glutamine, and histidine synthesis. Furthermore, an analysis of physiological data showed that B. breve BB8dpH displayed higher production of exopolysaccharide and lower H(+)-ATPase activity than B. breve BB8. The results presented here will improve our understanding of acid tolerance in bifidobacteria, and they will lead to the development of new strategies to enhance the acid tolerance of bifidobacterial strains.

  6. OpaR Controls a Network of Downstream Transcription Factors in Vibrio parahaemolyticus BB22OP

    PubMed Central

    Kernell Burke, Alison; Guthrie, Leah T. C.; Modise, Thero; Cormier, Guy; Jensen, Roderick V.; McCarter, Linda L.; Stevens, Ann M.

    2015-01-01

    Vibrio parahaemolyticus is an emerging world-wide human pathogen that is associated with food-borne gastroenteritis when raw or undercooked seafood is consumed. Expression of virulence factors in this organism is modulated by the phenomenon known as quorum sensing, which permits differential gene regulation at low versus high cell density. The master regulator of quorum sensing in V. parahaemolyticus is OpaR. OpaR not only controls virulence factor gene expression, but also the colony and cellular morphology associated with growth on a surface and biofilm formation. Whole transcriptome Next Generation sequencing (RNA-Seq) was utilized to determine the OpaR regulon by comparing strains BB22OP (opaR+, LM5312) and BB22TR (∆opaR1, LM5674). This work, using the published V. parahaemolyticus BB22OP genome sequence, confirms and expands upon a previous microarray analysis for these two strains that used an Affymetrix GeneChip designed from the closely related V. parahaemolyticus RIMD2210633 genome sequence. Overall there was excellent correlation between the microarray and RNA-Seq data. Eleven transcription factors under OpaR control were identified by both methods and further confirmed by quantitative reverse transcription PCR (qRT-PCR) analysis. Nine of these transcription factors were demonstrated to be direct OpaR targets via in vitro electrophoretic mobility shift assays with purified hexahistidine-tagged OpaR. Identification of the direct and indirect targets of OpaR, including small RNAs, will enable the construction of a network map of regulatory interactions important for the switch between the nonpathogenic and pathogenic states. PMID:25901572

  7. Poly I:C induces development of diabetes mellitus in BB rat.

    PubMed

    Sobel, D O; Newsome, J; Ewel, C H; Bellanti, J A; Abbassi, V; Creswell, K; Blair, O

    1992-04-01

    Polyinosinic polycytidilic acid (poly I:C), an inducer of alpha-interferon, accelerates the development of diabetes in diabetes-prone (DP) BioBreeding (BB) rats. This study investigates the effect of administering poly I:C to a diabetes-resistant (DR) strain of BB rats. We compared the incidence of diabetes, the degree of insulitis, the number of NK cells, helper-inducer cells, cytotoxic-suppressor cells, Ia+ T cells, RT6.1+ T cells, and NK cell bioactivity in DR rats treated with saline and with a 5 micrograms/g body wt (poly-5) dose and a 10 micrograms/g body wt (poly-10) dose of poly I:C. The incidence of diabetes was also compared with that of DP rats receiving poly-5. We found that both doses of poly I:C significantly induce the development of diabetes in the DR BB rat. However, treatment of DR rats with the higher dose induces a greater rate of development of diabetes and earlier onset of diabetes than the lower poly-5 dose. The rate of diabetes development and the mean age of onset were similar in poly-10-treated DR and poly-5-treated DP rats. A significant degree of insulitis occurred in all the poly I:C-treated DR rats, even those not developing diabetes. Peripheral blood NK cell number was greater in poly I:C than in saline-treated rats, after 2 wk of treatment and when killed. The percentage of OX19+ peripheral blood mononuclear cells expressing RT6.1 allotype or Ia antigen were similar in poly I:C- and saline-treated rats.(ABSTRACT TRUNCATED AT 250 WORDS)

  8. Atorvastatin calcium inhibits phenotypic modulation of PDGF-BB-induced VSMCs via down-regulation the Akt signaling pathway.

    PubMed

    Chen, Shuang; Liu, Baoqin; Kong, Dehui; Li, Si; Li, Chao; Wang, Huaqin; Sun, Yingxian

    2015-01-01

    Plasticity of vascular smooth muscle cells (VSMCs) plays a central role in the onset and progression of proliferative vascular diseases. In adult tissue, VSMCs exist in a physiological contractile-quiescent phenotype, which is defined by lack of the ability of proliferation and migration, while high expression of contractile marker proteins. After injury to the vessel, VSMC shifts from a contractile phenotype to a pathological synthetic phenotype, associated with increased proliferation, migration and matrix secretion. It has been demonstrated that PDGF-BB is a critical mediator of VSMCs phenotypic switch. Atorvastatin calcium, a selective inhibitor of 3-hydroxy-3-methyl-glutaryl l coenzyme A (HMG-CoA) reductase, exhibits various protective effects against VSMCs. In this study, we investigated the effects of atorvastatin calcium on phenotype modulation of PDGF-BB-induced VSMCs and the related intracellular signal transduction pathways. Treatment of VSMCs with atorvastatin calcium showed dose-dependent inhibition of PDGF-BB-induced proliferation. Atorvastatin calcium co-treatment inhibited the phenotype modulation and cytoskeleton rearrangements and improved the expression of contractile phenotype marker proteins such as α-SM actin, SM22α and calponin in comparison with PDGF-BB alone stimulated VSMCs. Although Akt phosphorylation was strongly elicited by PDGF-BB, Akt activation was attenuated when PDGF-BB was co-administrated with atorvastatin calcium. In conclusion, atorvastatin calcium inhibits phenotype modulation of PDGF-BB-induced VSMCs and activation of the Akt signaling pathway, indicating that Akt might play a vital role in the modulation of phenotype.

  9. PSM, a mediator of PDGF-BB-, IGF-I-, and insulin-stimulated mitogenesis.

    PubMed

    Riedel, H; Yousaf, N; Zhao, Y; Dai, H; Deng, Y; Wang, J

    2000-01-06

    PSM/SH2-B has been described as a cellular partner of the FcepsilonRI receptor, insulin receptor (IR), insulin-like growth factor-I (IGF-I) receptor (IGF-IR), and nerve growth factor receptor (TrkA). A function has been proposed in neuronal differentiation and development but its role in other signaling pathways is still unclear. To further elucidate the physiologic role of PSM we have identified additional mitogenic receptor tyrosine kinases as putative PSM partners including platelet-derived growth factor (PDGF) receptor (PDGFR) beta, hepatocyte growth factor receptor (Met), and fibroblast growth factor receptor. We have mapped Y740 as a site of PDGFR beta that is involved in the association with PSM. We have further investigated the putative role of PSM in mitogenesis with three independent experimental strategies and found that all consistently suggested a role as a positive, stimulatory signaling adapter in normal NIH3T3 and baby hamster kidney fibroblasts. (1) PSM expression from cDNA using an ecdysone-regulated transient expression system stimulated PDGF-BB-, IGF-I-, and insulin- but not EGF-induced DNA synthesis in an ecdysone dose-responsive fashion; (2) Microinjection of the (dominant negative) PSM SH2 domain interfered with PDGF-BB- and insulin-induced DNA synthesis; and (3) A peptide mimetic of the PSM Pro-rich putative SH3 domain-binding region interfered with PDGF-BB-, IGF-I-, and insulin- but not with EGF-induced DNA synthesis in NIH3T3 fibroblasts. This experiment was based on cell-permeable fusion peptides with the Drosophila antennapedia homeodomain which effectively traverse the plasma membrane of cultured cells. These experimental strategies independently suggest that PSM functions as a positive, stimulatory, mitogenic signaling mediator in PDGF-BB, IGF-I, and insulin but not in EGF action. This function appears to involve the PSM SH2 domain as well as the Pro-rich putative SH3 domain binding region. Our findings support the model that PSM

  10. Computerised emission and susceptibility MIL.STD testing with automated NB/BB detection

    NASA Astrophysics Data System (ADS)

    Vanessen, J. C.

    1990-09-01

    Automation of Electromagnetic Compatibility (EMC) testing is becoming common at many EMC test facilities. Commercial automated systems have become available in the past few years. The test and operations section has developed its own EMC automation to enhance and aid in testing. A complete overview of the automated EMC test facility in operation for emission and susceptibility measurements is presented. It includes a hardware description, the program structure and some of the methods required to complete such a program on the equipment chosen, including the Narrow Band (NB) and Broad Band (BB).

  11. Monte-Carlo Modeling of BB Emissivity and Its Monitoring Techniques in Support of the CLARREO Mission

    NASA Astrophysics Data System (ADS)

    Hanssen, L. M.; Prokhorov, A.; Wilthan, B.; Mekhontsev, S.; Khromchenko, V.; Zeng, J.

    2009-12-01

    A chief goal of the Climate Absolute Radiance and Refractivity Observatory (CLARREO) mission is the measurement of earth’s infrared emitted radiance. The primary method envisaged for SI traceability is through an onboard variable temperature reference blackbody (BB) source. The two quantities that determine the BB spectral radiance, via the Planck equation, are the BB temperature and its effective spectral emissivity. The temperature is determined through the monitoring of built in fixed-point cells. The emissivity can be determined pre-flight through both modeling and measurement, while it will be monitored on-orbit using one of several candidate methods. The Optical Technology Division at NIST has begun a comprehensive effort to support the establishment of the traceability path via both experimental calibrations and modeling. In this paper we present the results a pair of demonstration studies, based on Monte-Carlo modeling, of both the pre-flight determination and on-orbit monitoring of the CLARREO reference BB’s infrared spectral emissivity. (HU) For the pre-flight demonstration, test BB units from the University of Wisconsin’s Space Science and Engineering Center (SSEC) and Harvard University were modeled and measured experimentally. For input to the Monte-Carlo BB model, samples of the BB coatings were measured using NIST’s facilities for infrared spectral reflectance and bi-directional reflectance distribution function (BRDF). For cross-validation purposes, modeling results are compared to values obtained by separate measurements. Monte-Carlo modeling of two on-board BB emissivity monitoring schemes has also been performed. One involves monitoring the reflected radiation of the reference BB cavity, having been irradiated by quantum cascade laser sources. The second involves monitoring the reflected radiation, in the case of illumination by a thermal source that nearly fills the hemisphere in front of the cavity aperture. In each case the reflected

  12. Improved measurement of the bb production cross section in 920 GeV fixed-target proton-nucleus collisions

    SciTech Connect

    Abt, I.; Kisel, I.; Adams, M.; Cruse, C.; Ehret, K.; Funcke, M.; Schwenninger, B.; Wegener, D.; Agari, M.; Bauer, C.; Braeuer, M.; Hofmann, W.; Jagla, T.; Knoepfle, K.T.; Pleier, M.A.; Reeves, K.; Sanchez, F.; Schmelling, M.; Schwingenheuer, B.; Sciacca, F.

    2006-03-01

    A new measurement of the bb production cross section in 920 GeV proton-nucleus collisions is presented by the HERA-B Collaboration. The bb production is tagged via inclusive bottom quark decays into J/{psi} mesons by exploiting the longitudinal separation of J/{psi}{yields}l{sup +}l{sup -} decay vertices from the primary proton-nucleus interaction point. Both e{sup +}e{sup -} and {mu}{sup +}{mu}{sup -} channels are reconstructed for a total of 83{+-}12 inclusive b{yields}J/{psi}X events found. The combined analysis yields a bb to prompt J/{psi} cross section ratio of ({delta}{sigma}(bb)/{delta}{sigma}{sub J/{psi}})=0.032{+-}0.005{sub stat}{+-}0.004{sub =} s{sub ys} measured in the x{sub F} acceptance (-0.35bb)=14.9{+-}2.2{sub stat}{+-}2.4{sub sys} nucleon in the total phase space.

  13. BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties.

    PubMed

    Kayano, Anderson M; Simões-Silva, Rodrigo; Medeiros, Patrícia S M; Maltarollo, Vinícius G; Honorio, Kathia M; Oliveira, Eliandre; Albericio, Fernando; da Silva, Saulo L; Aguiar, Anna Caroline C; Krettli, Antoniana U; Fernandes, Carla F C; Zuliani, Juliana P; Calderon, Leonardo A; Stábeli, Rodrigo G; Soares, Andreimar M

    2015-11-01

    This study describes the biochemical and functional characterization of a new metalloproteinase named BbMP-1, isolated from Bothrops brazili venom. BbMP-1 was homogeneous on SDS-PAGE, presented molecular mass of 22,933Da and pI 6.4. The primary structure was partially elucidated with high identity with others metalloproteinases from Viperidae venoms. The enzymatic activity on azocasein was evaluated in different experimental conditions (pH, temperature). A significant reduction in enzyme activity after exposure to chelators of divalent cations (EDTA), reducing agents (DTT), pH less than 5.0 or temperatures higher than 45 °C was observed. BbMP-1 showed activity on fibrinogen degrading Aα chain quickly and to a lesser extent the Bβ chain. Also demostrated to be weakly hemorrhagic, presenting however, significant myotoxic and edematogenic activity. The in vitro activity of BbMP-1 against Plasmodium falciparum showed an IC50 of 3.2 ± 2.0 μg/mL. This study may help to understand the pathophysiological effects induced by this group of toxin and their participation in the symptoms observed in cases of snake envenomation. Moreover, this result is representative for this group of proteins and shows the biotechnological potential of BbMP-1 by the demonstration of its antiplasmodial activity.

  14. Intramuscular and topical treatment of cutaneous leishmaniasis lesions in mice infected with Leishmania amazonensis using coumarin (-) mammea A/BB.

    PubMed

    Tiuman, Tatiana Shioji; Brenzan, Mislaine Adriana; Ueda-Nakamura, Tânia; Filho, Benedito Prado Dias; Cortez, Diógenes Aparicio Garcia; Nakamura, Celso Vataru

    2012-10-15

    Treatment of cutaneous leishmaniasis remains limited to a few available options. Recent studies showed in vitro antileishmanial activity of (-) mammea A/BB, a coumarin isolated from leaves of Calophyllum brasiliense. Moreover, the dichloromethane crude extract and hexane fraction from this plant demonstrated in vivo activity in mice infected with Leishmania amazonensis. We evaluated the antileishmanial activity of (-) mammea A/BB in the L. amazonensis BALB/c mice model. The animals were given intramuscular and topical treatment with (-) mammea A/BB for 30 consecutive days. The results demonstrated that 18mg/kg/d intramuscularly or 0.2% topically of (-) mammea A/BB significantly reduced the size of skin lesions in footpads of mice compared with those in the control group (p<0.05). The activity of Glucantime(®) (corresponding to 27mg/kg/d of pentavalent antimony) administered intramuscularly was similar to that of (-) mammea A/BB (p<0.05) by both routes of administration. The histopathological evaluation showed no changes in the organs analyzed. These results indicate that the coumarin obtained from C. brasiliense is the antileishmanially active compound and can be used to control the development of cutaneous leishmaniasis lesions caused by L. amazonensis.

  15. Major histocompatibility complex gene product expression on pancreatic beta cells in acutely diabetic BB rats.

    PubMed Central

    Issa-Chergui, B.; Yale, J. F.; Vigeant, C.; Seemayer, T. A.

    1988-01-01

    Type I diabetes mellitus was induced in young, diabetes-prone BB rats by the passive transfer of concanavalin A-activated T lymphocytes from the spleens of acutely diabetic BB rats. The pancreas of the recipients was examined 1-2 days after the onset of glycosuria by immunocytochemistry by means of monoclonal antibodies for determining whether 1) Class I and/or II major histocompatibility gene complex (MHC) products were expressed on beta cells and 2) the mononuclear cell infiltrates were represented by T cells. Marked expression of Class I MHC gene products was evident on beta cells. In contrast, Class II MHC gene products were not identified on normal-appearing beta cells. Dendritic cells dispersed throughout the acinar and interstitial pancreas were markedly increased in number. The mononuclear cell infiltrate contained few cells (1-15%) recognized by a pan-T cell marker. Although it is possible that this passive transfer model might differ considerably from the spontaneously occurring diabetic state in the rat, this study suggests that 1) Class I, rather than Class II, MHC gene expression may be pivotal to beta-cell injury in diabetic rats, and 2) non-T cells may constitute an effector cell population central to beta-cell necrosis in Type I diabetes mellitus. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:3276208

  16. The matrix metalloproteinase inhibitor BB-1101 prevents experimental autoimmune uveoretinitis (EAU).

    PubMed

    Wallace, G R; Whiston, R A; Stanford, M R; Wells, G M; Gearing, A J; Clements, J M

    1999-12-01

    EAU is characterized by breakdown of the blood-retinal barrier and extravasation of leucocytes into retinal tissue leading to destruction of photoreceptor cells. Matrix metalloproteinases (MMP) have been implicated in trafficking of cells into tissues, but their role in inflammatory eye disease is unclear. A synthetic MMP inhibitor, BB-1101, was administered subcutaneously, from either day 0 or day 7, to Lewis rats challenged with bovine S-antigen to induce EAU. When given up to day 14, BB-1101 reduced the incidence of disease and delayed the day of onset of clinical disease. When administered from day 7 until day 21, EAU was completely abrogated. A quantitative polymerase chain reaction (PCR) assay showed an increase of both matrilysin (MMP-7), neutrophil collagenase (MMP-8) and macrophage metalloproteinase (MMP-12) in retinas from EAU animals compared with naive controls. These enzymes are produced by activated leucocytes and act on components of the basement membrane. These results therefore implicate these MMP as integral to the development of pathology in EAU.

  17. The matrix metalloproteinase inhibitor BB-1101 prevents experimental autoimmune uveoretinitis (EAU)

    PubMed Central

    Wallace, G R; Whiston, R A; Stanford, M R; Wells, G M A; Gearing, A J H; Clements, J M

    1999-01-01

    EAU is characterized by breakdown of the blood–retinal barrier and extravasation of leucocytes into retinal tissue leading to destruction of photoreceptor cells. Matrix metalloproteinases (MMP) have been implicated in trafficking of cells into tissues, but their role in inflammatory eye disease is unclear. A synthetic MMP inhibitor, BB-1101, was administered subcutaneously, from either day 0 or day 7, to Lewis rats challenged with bovine S-antigen to induce EAU. When given up to day 14, BB-1101 reduced the incidence of disease and delayed the day of onset of clinical disease. When administered from day 7 until day 21, EAU was completely abrogated. A quantitative polymerase chain reaction (PCR) assay showed an increase of both matrilysin (MMP-7), neutrophil collagenase (MMP-8) and macrophage metalloproteinase (MMP-12) in retinas from EAU animals compared with naive controls. These enzymes are produced by activated leucocytes and act on components of the basement membrane. These results therefore implicate these MMP as integral to the development of pathology in EAU. PMID:10594553

  18. Inclusive b-jet and bb¯-dijet production at the LHC via Reggeized gluons

    NASA Astrophysics Data System (ADS)

    Saleev, V. A.; Shipilova, A. V.

    2012-08-01

    We study inclusive b-jet and bb¯-dijet production at the CERN LHC invoking the hypothesis of gluon Reggeization in t-channel exchanges at high energy. The b-jet cross section includes contributions from open b-quark production and from b-quark production via gluon-to-bottom-pair fragmentation. The transverse-momentum distributions of inclusive b-jet production measured with the ATLAS detector at the CERN LHC in different rapidity ranges are calculated both within multi-Regge kinematics and quasi-multi-Regge kinematics. The bb¯-dijet cross section is calculated within quasi-multi-Regge kinematics as a function of the dijet invariant mass Mjj, the azimuthal angle between the two jets Δϕ, and the angular variable χ. At the numerical calculation, we adopt the Kimber-Martin-Ryskin and Blümlein prescriptions to derive unintegrated gluon distribution function of the proton from its collinear counterpart for which we use the Martin-Roberts-Stirling-Thorne set. We find good agreement with measurements by the ATLAS and CMS Collaborations at the LHC at the hadronic c.m. energy of S=7TeV.

  19. The cellular proteome is affected by a gelsolin (BbGEL1) during morphological transitions in aerobic surface versus liquid growth in the entomopathogenic fungus Beauveria bassiana.

    PubMed

    He, Pu-Hong; Dong, Wei-Xia; Chu, Xin-Ling; Feng, Ming-Guang; Ying, Sheng-Hua

    2016-11-01

    The gelsolin superfamily includes seven protein members: gelsolin, villin, adseverin, CapG, advillin, supervillin and flightless I. The gelsolin proteins are actin-binding proteins that contain three or six gelsolin-like domains, and they play important roles in remodelling actin dynamics and cellular processes in eukaryotes. The entomopathogenic fungus Beauveria bassiana expresses a unique CapG protein (BbGEL1) that contains three gelsolin-like domains. BbGEL1p is associated with actin during mycelial growth and plays an important role in fungal morphological transitions under both aerobic and submerged conditions. The ΔBbGEL1 mutant displays abnormal spore-producing structures that reduce the conidial and blastospore yields by approximately 70% and 90% respectively. The virulence of the ΔBbGEL1 mutant is notably reduced as indicated by topical and intrahemocoel injection assays. Two comparative proteomics analyses indicated that BbGEL1 has significantly different roles in the development of conidia and blastospores, and the results revealed the potential targets of BbGEL1 in the corresponding developmental processes. Additionally, as an overlapping downstream protein of BbGEL1, the hydrophobin-like protein gene BbHyd3 is required for conidiation but has a negative role in blastospore formation. Our findings indicate that in addition to its function as an actin-interacting protein, BbGEL1 contributes to fungal morphological transitions via broad genetic pathways.

  20. Pseudomonas syringae Type III Effector HopBB1 Promotes Host Transcriptional Repressor Degradation to Regulate Phytohormone Responses and Virulence.

    PubMed

    Yang, Li; Teixeira, Paulo José Pereira Lima; Biswas, Surojit; Finkel, Omri M; He, Yijian; Salas-Gonzalez, Isai; English, Marie E; Epple, Petra; Mieczkowski, Piotr; Dangl, Jeffery L

    2017-02-08

    Independently evolved pathogen effectors from three branches of life (ascomycete, eubacteria, and oomycete) converge onto the Arabidopsis TCP14 transcription factor to manipulate host defense. However, the mechanistic basis for defense control via TCP14 regulation is unknown. We demonstrate that TCP14 regulates the plant immune system by transcriptionally repressing a subset of the jasmonic acid (JA) hormone signaling outputs. A previously unstudied Pseudomonas syringae (Psy) type III effector, HopBB1, interacts with TCP14 and targets it to the SCF(COI1) degradation complex by connecting it to the JA signaling repressor JAZ3. Consequently, HopBB1 de-represses the TCP14-regulated subset of JA response genes and promotes pathogen virulence. Thus, HopBB1 fine-tunes host phytohormone crosstalk by precisely manipulating part of the JA regulon to avoid pleiotropic host responses while promoting pathogen proliferation.

  1. A first-look atmospheric modeling study of the young directly imaged planet-mass companion, ROXS 42Bb

    SciTech Connect

    Currie, Thayne; Daemgen, Sebastian; Burrows, Adam

    2014-06-01

    We present and analyze JK{sub s}L' photometry and our previously published H-band photometry and K-band spectroscopy for ROXs 42Bb, an object Currie et al. first reported as a young directly imaged planet-mass companion. ROXs 42Bb exhibits IR colors redder than field L dwarfs but consistent with other planet-mass companions. From the H{sub 2}O-2 spectral index, we estimate a spectral type of L0 ± 1; weak detections/non-detections of the CO bandheads, Na I, and Ca I support evidence for a young, low surface gravity object primarily derived from the H{sub 2}(K) index. ROXs 42Bb's photometry/K-band spectrum are inconsistent with limiting cases of dust-free atmospheres (COND) and marginally inconsistent with the AMES/DUSTY models and the BT-SETTL models. However, ROXS 42Bb data are simultaneously fit by atmosphere models incorporating several micron-sized dust grains entrained in thick clouds, although further modifications are needed to better reproduce the K-band spectral shape. ROXs 42Bb's best-estimated temperature is T {sub eff} ∼ 1950-2000 K, near the low end of the empirically derived range in Currie et al. For an age of ∼1-3 Myr and considering the lifetime of the protostar phase, ROXs 42Bb's luminosity of log(L/L {sub ☉}) ∼ –3.07 ± 0.07 implies a mass of 9{sub −3}{sup +3} M{sub J} , making it one of the lightest planetary-mass objects yet imaged.

  2. A First-look Atmospheric Modeling Study of the Young Directly Imaged Planet-mass Companion, ROXs 42Bb

    NASA Astrophysics Data System (ADS)

    Currie, Thayne; Burrows, Adam; Daemgen, Sebastian

    2014-06-01

    We present and analyze JKsL' photometry and our previously published H-band photometry and K-band spectroscopy for ROXs 42Bb, an object Currie et al. first reported as a young directly imaged planet-mass companion. ROXs 42Bb exhibits IR colors redder than field L dwarfs but consistent with other planet-mass companions. From the H2O-2 spectral index, we estimate a spectral type of L0 ± 1; weak detections/non-detections of the CO bandheads, Na I, and Ca I support evidence for a young, low surface gravity object primarily derived from the H2(K) index. ROXs 42Bb's photometry/K-band spectrum are inconsistent with limiting cases of dust-free atmospheres (COND) and marginally inconsistent with the AMES/DUSTY models and the BT-SETTL models. However, ROXS 42Bb data are simultaneously fit by atmosphere models incorporating several micron-sized dust grains entrained in thick clouds, although further modifications are needed to better reproduce the K-band spectral shape. ROXs 42Bb's best-estimated temperature is T eff ~ 1950-2000 K, near the low end of the empirically derived range in Currie et al. For an age of ~1-3 Myr and considering the lifetime of the protostar phase, ROXs 42Bb's luminosity of log(L/L ⊙) ~ -3.07 ± 0.07 implies a mass of 9^{+3}_{-3} MJ , making it one of the lightest planetary-mass objects yet imaged.

  3. Phase diagram of the B-B2O3 system at 5 GPa: experimental and theoretical studies.

    PubMed

    Solozhenko, Vladimir L; Kurakevych, Oleksandr O; Turkevich, Vladimir Z; Turkevich, Dmitry V

    2008-05-29

    X-ray diffraction with synchrotron radiation has been used to study in situ the chemical interaction of beta-rhombohedral boron with boron (III) oxide and phase relations in the B-B2O3 system at pressures up to 6 GPa in the temperature range from 300 to 2800 K. The B-B2O3 system has been thermodynamically analyzed, and its equilibrium phase diagram at 5 GPa has been constructed. Only one thermodynamically stable boron suboxide, B6O, exists in the system. It forms eutectic equilibria with boron and B2O3.

  4. PI3K/Akt responses to oxytocin stimulation in Caco2BB gut cells.

    PubMed

    Klein, Benjamin Y; Tamir, Hadassah; Welch, Martha G

    2011-11-01

    Recently, we discovered oxytocin receptor (OTR) expression in the developing gut villus epithelium that emerges in villus-crypt junctions after weaning. Oxytocin (OT) and OTR regulate many physiological functions in various tissues; however, their function in gut epithelium is unknown. We explored responses of PI3K and Akt phosphoisoforms to OT stimuli in the Caco2BB human gut cell line. In Caco2BB cells, PI3K and pAkt levels peaked at 62.5  nM OT. At higher concentrations, PI3K decreased more gradually than pAkt(S473) suggesting that the pAkt(S473) response is separate from PI3K. At ≤7.8  nM OT, pAkt(T308) increased while pAkt(S473) decreased. Using a specific OTR antagonist, we demonstrated that responses of pAkt(T308) to OT depend on OTR in contrast to the partial OTR-dependence of the pAkt(S473) response. Differential pAkt phosphoisoform responses included pAkt phosphoserine 473 persistently free of phosphothreonine 308. The reduction in PI3K after 62.5  nM OT for 30  min coincided with OTR internalization. The PI3K/Akt activation profile was somewhat different in other cell lines (MCF-7 breast cancer cells, HT29 gut cells), which have PI3K activating mutations, that were examined to establish experimental parameters. In Caco2BB cells, the divergent effects of OT upon pAkt phosphoisoforms suggests separate sub-pathways; pAkt (T308) activation depends on OTR via the PI3K pathway and pAkt(S473) presumably results from its specific kinase mTORC2 (mammalian target of rapamycin complex 2). Thus, OT may modulate gut cell functions downstream of mTOR complexes (e.g., translation control as suggested by others in uterine cells). We will next explore OT-stimulated kinase activities downstream of mTOR related to pAkt phosphoisoforms.

  5. T cell dysfunction in the diabetes-prone BB rat. A role for thymic migrants that are not T cell precursors

    SciTech Connect

    Georgiou, H.M.; Lagarde, A.C.; Bellgrau, D.

    1988-01-01

    Diabetes-prone BB (BB-DP) rats express several T cell dysfunctions which include poor proliferative and cytotoxic responses to alloantigen. The goal of this study was to determine the origin of these T cell dysfunctions. When BB-DP rats were thymectomized, T cell depleted, and transplanted with neonatal thymus tissue from diabetes-resistant and otherwise normal DA/BB F1 rats, the early restoration of T cell function proceeded normally on a cell-for-cell basis; i.e., peripheral T cells functioned like those from the thymus donor. Because the thymus in these experiments was subjected to gamma irradiation before transplantation and there was no evidence of F1 chimerism in the transplanted BB-DP rats, it appeared that the BB-DP T cell precursors could mature into normally functioning T cells if the maturation process occurred in a normal thymus. If the F1 thymus tissue was treated with dGua before transplantation, the T cells of these animals functioned poorly like those from untreated BB-DP rats. dGua poisons bone marrow-derived cells, including gamma radiation-resistant cells of the macrophage/dendritic cell lineages, while sparing the thymic epithelium. Therefore, the reversal of the T cell dysfunction depends on the presence in the F1 thymus of gamma radiation-resistant, dGua-sensitive F1 cells. Conversely, thymectomized and T cell-depleted F1 rats expressed T cell dysfunction when transplanted with gamma-irradiated BB thymus grafts. T cell responses were normal in animals transplanted with dGua-treated BB thymus grafts. With increasing time after thymus transplantation, T cells from all animals gradually expressed the functional phenotype of the bone marrow donor. Taken together these results suggest that BB-DP bone marrow-derived cells that are not T cell precursors influence the maturation environment in the thymus of otherwise normal BB-DP T cell precursors.

  6. Prevention of diabetes mellitus in the BB/W rat with Cyclosporin-A.

    PubMed Central

    Like, A. A.; Dirodi, V.; Thomas, S.; Guberski, D. L.; Rossini, A. A.

    1984-01-01

    Autoimmune diabetes mellitus occurs spontaneously in 40-60% of a colony of BioBreeding/Worcester rats. Pretreatment of susceptible animals for 10-day intervals prior to 70 days of age with Cyclosporin-A (CSA) significantly reduced the frequency and delayed the onset of diabetes. The relatively narrow time frame of successful treatment suggests that effector cells responsible for beta cell destruction in this model of Type I diabetes may be activated during this period of time prior to the onset of overt hyperglycemia. CSA administration did not protect against the occurrence of lymphocytic thyroiditis or autoantibodies directed against smooth muscle or thyroid colloid, suggesting that these BB immunologic phenomena may be controlled by a distinct series of immunologic events. PMID:6385729

  7. Heavy quarkonium ππ transitions and a possible bb¯qq¯ state

    NASA Astrophysics Data System (ADS)

    Guo, F.-K.; Shen, P.-N.; Chiang, H.-C.; Ping, R.-G.

    2005-11-01

    ππ transitions of heavy quarkonia, especially the ϒ(3S)→ϒ(1S)ππ decay process, are revisited. In the framework of the Chiral Unitary Theory (ChUT), the S wave ππ final state interaction (FSI) is included. It is found that when an additional intermediate state with J=1 and I=1 is introduced, not only the ππ invariant mass spectrum and the cosθπ∗ distribution in the ϒ(3S)→ϒ(1S)ππ process can simultaneously be well explained, but also a consistent description for other bottomonia ππ transitions can be obtained. As a consequence, the mass and the width of the intermediate state are predicted. From the quark content analysis, this state should be a bb¯qq¯ state.

  8. Production of haploids from anther culture of banana [Musa balbisiana (BB)].

    PubMed

    Assani, A; Bakry, F; Kerbellec, F; Haïcour, R; Wenzel, G; Foroughi-Wehr, B

    2003-02-01

    We report here, for the first time, the production of haploid plants of banana Musa balbisiana (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype Pisang klutuk, 12 from Pisang batu, seven from Pisang klutuk wulung and four from Tani. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes.

  9. The discovery and development of the BB rat colony: an animal model of spontaneous diabetes mellitus.

    PubMed

    Chappel, C I; Chappel, W R

    1983-07-01

    The BB rat model of spontaneous diabetes mellitus was discovered in 1974 in Ottawa in a colony of specific pathogen-free Wistar rats. Investigations to determine the cause of rapid weight loss and death in a few weanling rats from this colony revealed polydypsia, polyuria, glucosuria, ketonuria, and hyperglycemia. These signs regressed and normal weight gain occurred when daily insulin therapy was given. Histologic studies of the pancreas of affected animals showed fibrosis and absence of beta cells. The original colony was established by crossbreeding the clinically normal parents of the diabetic animals. Approximately 10% of the offspring of these matings became diabetic. This incidence was increased to approximately 25% by father-daughter mating, suggesting a genetic component in the etiology.

  10. Comparison of direct, rehalogenating, and solvent bleaching processes with BB-640 plates

    NASA Astrophysics Data System (ADS)

    Ulibarrena, Manuel; Mendez, Maria J.; Carretero, Luis; Madrigal, Roque; Fimia, Antonio

    2002-07-01

    Bleaching is one of the key processes in the production of phase holograms with fine grain silver halide emulsions. This step was widely studied for discontinued Kodak, Agfa, and Ilford emulsions, and most of those results have been optimized with small changes for presently available holographic emulsions. A study of the different bleaching techniques with BB-640 holographic emulsions is presented. Some interesting new features found are the high-diffraction efficiencies achieved with direct rehalogenating bleaching and with reversal bleaching, with higher values and better sensitivities than those of classical fixation-free rehalogenating bleaching. In particular, we have found that the highest diffraction efficiency and the lowest scattering is obtained with AAC developer and reversal bleach R-9, with diffraction efficiency values of 82%.

  11. Thick phase holographic gratings recorded on BB-640 and PFG-01 silver halide materials

    NASA Astrophysics Data System (ADS)

    Neipp, Cristian; Márquez, Andrés; Pascual, Inmaculada; Beléndez, Augusto

    2003-09-01

    Photographic emulsions are still widely used to record holographic optical elements. In particular, if high diffraction efficiencies are needed, phase volume holograms are preferably recorded on these emulsions. Two particular techniques producing high-quality volume phase holograms are fixation-free rehalogenating bleaching and the use of silver-halide-sensitized gelatin. In this work we compare these two particular techniques applied to three different red-sensitive emulsions: Agfa 8E75 HD, BB-640, and Slavich PFG-01 emulsions. The differences between these emulsions determine the particular experimental conditions needed to record high-quality volume holograms on them. In this work these differences are analysed, and also their influence on the experimental procedure.

  12. Nonlinear effects on holographic reflection gratings recorded with BB640 emulsions.

    PubMed

    Ulibarrena, Manuel; Carretero, Luis; Madrigal, Roque; Blaya, Salvador; Fimia, Antonio

    2003-08-11

    Nonlinear recording effects on holographic re ection gratings recorded on BB640 emulsions have been studied. Using an analytical approach to the characteristic curve of this emulsions, the density profiles recorded in the developed emulsions that correspond to the original light pattern generated by the holographic setup have been obtained. The final spatial profiles of the refraction index resulting from different exposure levels and different bleaching processes have been evaluated studying the experimental diffraction efficiency and comparing it with Kogelnik's theory. The quality of the fittings of this model with the experimental spectral responses of the final gratings has been used as a measure of the accordance between the original sinusoidal and periodical light distribution and the obtained modulation profile of the processed hologram. The range of applicability of the theoretical model to the experimental results has been evaluated using different bleaching processes.

  13. Quorum sensing in metal tolerance of Acinetobacter junii BB1A is associated with biofilm production.

    PubMed

    Sarkar, Suchitra; Chakraborty, Ranadhir

    2008-05-01

    Acinetobacter junii strain BB1A, a novel metal-tolerant bacterium, produced biofilm in the presence of added ions such as Ni(2+), AsO(2)(-), Cd(2+) and Hg(2+) on surfaces such as glass and polystyrene. Generation of a metal-sensitive and adhesion-deficient mutant by transposition of Tn5-mob in the A. junii genome has putatively confirmed the association of metal tolerance with the production of biofilm. The requirement of a critical cell density for biofilm formation and presence of acyl-homoserine lactone-like autoinducer molecules in the cell-free supernatant indicated the phenomenon of quorum sensing. Addition of a natural quorum-sensing inhibitor (garlic extract) or synthetic quorum-sensing inhibitor (4-nitro-pyridine oxide) significantly inhibited cell growth and biofilm formation in the presence of metal/metalloid ions.

  14. Experimental tests of hidden variable theories from dBB to stochastic electrodynamics

    NASA Astrophysics Data System (ADS)

    Genovese, Marco; Brida, Giorgio; Gramegna, Marco; Piacentini, Fabrizio; Predazzi, Enrico; Ruo-Berchera, Ivano

    2007-05-01

    The studies concerning the possible existence of a deterministic theory, of which quantum mechanics would be an approximation, date to the celebrated 1935 Einstein-Podolsky-Rosen paper. Since Bell's proposal of 1964 various experiments were addressed to a general experimental test of local hidden variable theories, leading to strong indications favourable to Standard Quantum Mechanics. Nevertheless, detection loophole still persists. After a short presentation of recent PDC photon experiments, we will present our recent works in this field and, in particular, a conclusive negative test of stochastic electrodynamics. Finally, we will also mention possible tests of non-local deterministic models and give some detail on our test of the dBB model.

  15. On the optimality of individual entangling-probe attacks against BB84 quantum key distribution

    NASA Astrophysics Data System (ADS)

    Herbauts, I. M.; Bettelli, S.; Hã¼bel, H.; Peev, M.

    2008-02-01

    Some MIT researchers [Phys. Rev. A 75, 042327 (2007)] have recently claimed that their implementation of the Slutsky-Brandt attack [Phys. Rev. A 57, 2383 (1998); Phys. Rev. A 71, 042312 (2005)] to the BB84 quantum-key-distribution (QKD) protocol puts the security of this protocol “to the test” by simulating “the most powerful individual-photon attack” [Phys. Rev. A 73, 012315 (2006)]. A related unfortunate news feature by a scientific journal [G. Brumfiel, Quantum cryptography is hacked, News @ Nature (april 2007); Nature 447, 372 (2007)] has spurred some concern in the QKD community and among the general public by misinterpreting the implications of this work. The present article proves the existence of a stronger individual attack on QKD protocols with encrypted error correction, for which tight bounds are shown, and clarifies why the claims of the news feature incorrectly suggest a contradiction with the established “old-style” theory of BB84 individual attacks. The full implementation of a quantum cryptographic protocol includes a reconciliation and a privacy-amplification stage, whose choice alters in general both the maximum extractable secret and the optimal eavesdropping attack. The authors of [Phys. Rev. A 75, 042327 (2007)] are concerned only with the error-free part of the so-called sifted string, and do not consider faulty bits, which, in the version of their protocol, are discarded. When using the provably superior reconciliation approach of encrypted error correction (instead of error discard), the Slutsky-Brandt attack is no more optimal and does not “threaten” the security bound derived by Lütkenhaus [Phys. Rev. A 59, 3301 (1999)]. It is shown that the method of Slutsky and collaborators [Phys. Rev. A 57, 2383 (1998)] can be adapted to reconciliation with error correction, and that the optimal entangling probe can be explicitly found. Moreover, this attack fills Lütkenhaus bound, proving that it is tight (a fact which was not

  16. De Novo Whole-Genome Sequence of Xylella fastidiosa subsp. multiplex Strain BB01 Isolated from a Blueberry in Georgia, USA

    PubMed Central

    Van Horn, Christopher; Chang, Chung-Jan

    2017-01-01

    ABSTRACT This study reports a de novo-assembled draft genome sequence of Xylella fastidiosa subsp. multiplex strain BB01 causing blueberry bacterial leaf scorch in Georgia, USA. The BB01 genome is 2,517,579 bp, with a G+C content of 51.8%, 2,943 open reading frames (ORFs), and 48 RNA genes. PMID:28183766

  17. Sustaining neovascularization of a scaffold through staged release of vascular endothelial growth factor-A and platelet-derived growth factor-BB.

    PubMed

    Davies, Neil H; Schmidt, Christian; Bezuidenhout, Deon; Zilla, Peter

    2012-01-01

    Tissue regeneration into a three-dimensional scaffold requires the stimulation of blood vessel ingrowth. We have employed a freely interconnecting porous scaffold developed by us to determine the utility of a covalently bound heparin surface coating for the delivery of vascular endothelial growth factor (VEGF) and platelet-derived growth factor BB (PDGF-BB) in vivo. The heparin surface was shown to release VEGF far more rapidly than PDGF-BB in vitro (VEGF: 75 ng/h for 24 h; PDGF-BB: 86 pg/h for >7 days). In rat subcutaneous implants, at 10 days the heparin surface alone increased vessel ingrowth substantially (p<0.05 vs. unmodified scaffold), release of VEGF resulted in a further increase (p<0.05 vs. heparinized scaffold), whereas PDGF-BB had no additional effect. The increase induced by the combination of growth factors was similar to VEGF alone. After 2 months, PDGF-BB, but not VEGF delivery, resulted in a substantial increase in vascularization above that induced by heparin (p<0.05). At the longer time point the combination of growth factors was similar to PDGF-BB. However, only the combination of growth factors significantly elevated the number of ingrowing arterioles (p<0.05 vs. heparinized scaffold). Thus, the covalent modification of a porous scaffold with heparin allows for the differential release of VEGF and PDGF-BB that results in both a rapid and sustained increase in scaffold vascularization.

  18. 40 CFR 174.518 - Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Bacillus thuringiensis Cry3Bb1 protein... PLANT-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.518 Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance. Residues of Bacillus...

  19. 40 CFR 174.518 - Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in corn; exemption from the requirement of a tolerance. 174.518 Section 174.518 Protection of... Cry3Bb1 protein in corn; exemption from the requirement of a tolerance. Residues of Bacillus thuringiensis Cry3Bb1 protein in corn are exempt from the requirement of a tolerance when used as...

  20. 40 CFR 174.518 - Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in corn; exemption from the requirement of a tolerance. 174.518 Section 174.518 Protection of... Cry3Bb1 protein in corn; exemption from the requirement of a tolerance. Residues of Bacillus thuringiensis Cry3Bb1 protein in corn are exempt from the requirement of a tolerance when used as...

  1. 40 CFR 174.518 - Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in corn; exemption from the requirement of a tolerance. 174.518 Section 174.518 Protection of... Cry3Bb1 protein in corn; exemption from the requirement of a tolerance. Residues of Bacillus thuringiensis Cry3Bb1 protein in corn are exempt from the requirement of a tolerance when used as...

  2. 40 CFR 174.518 - Bacillus thuringiensis Cry3Bb1 protein in corn; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... in corn; exemption from the requirement of a tolerance. 174.518 Section 174.518 Protection of... Cry3Bb1 protein in corn; exemption from the requirement of a tolerance. Residues of Bacillus thuringiensis Cry3Bb1 protein in corn are exempt from the requirement of a tolerance when used as...

  3. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling.

    PubMed

    Barrett, Angela; Evans, Ian M; Frolov, Antonina; Britton, Gary; Pellet-Many, Caroline; Yamaji, Maiko; Mehta, Vedanta; Bandopadhyay, Rina; Li, Ningning; Brandner, Sebastian; Zachary, Ian C; Frankel, Paul

    2014-06-15

    DOK1 regulates platelet-derived growth factor (PDGF)-BB-stimulated glioma cell motility. Mechanisms regulating tumour cell motility are essential for invasion and metastasis. We report here that PDGF-BB-mediated glioma cell invasion and migration are dependent on the adaptor protein downstream of kinase 1 (DOK1). DOK1 is expressed in several glioma cell lines and in tumour biopsies from high-grade gliomas. DOK1 becomes tyrosine phosphorylated upon PDGF-BB stimulation of human glioma cells. Knockdown of DOK1 or expression of a DOK1 mutant (DOK1FF) containing Phe in place of Tyr at residues 362 and 398, resulted in inhibition of both the PDGF-BB-induced tyrosine phosphorylation of p130Cas (also known as BCAR1) and the activation of Rap1. DOK1 colocalises with tyrosine phosphorylated p130Cas at the cell membrane of PDGF-BB-treated cells. Expression of a non-tyrosine-phosphorylatable substrate domain mutant of p130Cas (p130Cas15F) inhibited PDGF-BB-mediated Rap1 activation. Knockdown of DOK1 and Rap1 inhibited PDGF-BB-induced chemotactic cell migration, and knockdown of DOK1 and Rap1 and expression of DOK1FF inhibited PDGF-mediated three-dimensional (3D) spheroid invasion. These data show a crucial role for DOK1 in the regulation of PDGF-BB-mediated tumour cell motility through a p130Cas-Rap1 signalling pathway. [Corrected

  4. 78 FR 13742 - BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that BB&T Capital...

  5. 76 FR 55720 - BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-08

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that BB&T Capital...

  6. Activation of bovine monocytes and neutrophils by the Bb fragment of complement factor B: demonstration by the uptake of 3H-deoxyglucose.

    PubMed Central

    Sethi, M S; Tabel, H; Misra, V

    1990-01-01

    The Bb fragment is the enzymatically active split product of bovine complement factor B. The Bb fragment was obtained after zymosan treatment of fresh bovine serum and fractionation of the treated serum, first over diethylaminoethyl-Sephacel and then over an affinity column made up of monoclonal antibody to bovine Bb, coupled to cyanogen-bromide-activated Sepharose. Purified Bb has a molecular weight of 64,000, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The ability of purified Bb to activate phagocytes was assessed. The activation assay was based on the principle that the primary source of energy for the phagocytes is obtained from glucose. 3H-deoxyglucose, a nonmetabolizable analogue of glucose, was used to obtain the quantitative measurement of the activation process. The activation by Bb was shown by the uptake of the labelled deoxyglucose in the phagocytic cells and was comparable to the activation caused by phorbol myristate acetate and N-formyl-L-methionyl-L-leucyl-L-phenylalanine, run in parallel. These data showed that fragment Bb activates bovine monocytes and neutrophils and also suggested that, when generated after complement activation, Bb may stimulate monocytes and neutrophils for enhanced phagocytosis. Images Fig. 1. Fig. 2. PMID:2306658

  7. 2008/09 Baccalaureate and Beyond Longitudinal Study (B&B:08/09). Full-Scale Methodology Report. NCES 2014-041

    ERIC Educational Resources Information Center

    Wine, Jennifer; Janson, Natasha; Siegel, Peter; Bennett, Christopher

    2013-01-01

    This report documents the methodological procedures and evaluations of the 2008/09 Baccalaureate and Beyond Longitudinal Study (B&B:08/09). RTI, with the assistance of MPR, conducted B&B:08/09 for the National Center for Education Statistics (NCES) of the U.S. Department of Education. Chapter 1 describes the background, legislative…

  8. A Multifunctional Role for Adjuvant Anti-4-1BB Therapy in Augmenting Antitumor Response by Chimeric Antigen Receptor T Cells.

    PubMed

    Mardiana, Sherly; John, Liza B; Henderson, Melissa A; Slaney, Clare Y; von Scheidt, Bianca; Giuffrida, Lauren; Davenport, Alexander J; Trapani, Joseph A; Neeson, Paul J; Loi, Sherene; Haynes, Nicole M; Kershaw, Michael H; Beavis, Paul A; Darcy, Phillip K

    2017-03-15

    Adoptive immunotherapy utilizing chimeric antigen receptor (CAR) T cells has demonstrated high success rates in hematologic cancers, but results against solid malignancies have been limited to date, due in part to the immunosuppressive tumor microenvironment. Activation of the 4-1BB (CD137) pathway using an agonistic α-4-1BB antibody is known to provide strong costimulatory signals for augmenting and diversifying T-cell responses. We therefore hypothesized that a combination of α-4-1BB and CAR T-cell therapy would result in improved antitumor responses. Using a human-Her2 self-antigen mouse model, we report here that α-4-1BB significantly enhanced CAR T-cell efficacy directed against the Her2 antigen in two different established solid tumor settings. Treatment also increased the expression of IFNγ and the proliferation marker Ki67 in tumor-infiltrating CAR T cells when combined with α-4-1BB. Strikingly, α-4-1BB significantly reduced host immunosuppressive cells at the tumor site, including regulatory T cells and myeloid-derived suppressor cells, correlating with an increased therapeutic response. We conclude that α-4-1BB has a multifunctional role for enhancing CAR T-cell responses and that this combination therapy has high translational potential, given current phase I/II clinical trials with α-4-1BB against various types of cancer. Cancer Res; 77(6); 1296-309. ©2017 AACR.

  9. Selection for Cry3Bb1 resistance in a genetically diverse population of nondiapausing Western Corn Rootworm (Coleoptera: Chrysomelidae)

    EPA Science Inventory

    Five short-diapause laboratory lines of western corn rootworm (Diabrotica virgifera virgifera) were selected for resistance to MON863, a variety of corn genetically modified with the Bacillus thuringiensis (Bt) transgene that expresses the Cry3Bb1 endotoxin. Three of the selecte...

  10. Isolation of transcripts from Diabrotica virgifera virgifera LeConte responsive to the Bacillus thuringiensis toxin Cry3Bb1

    EPA Science Inventory

    Crystal proteins derived from Bacillus thuringiensis (Bt) have been widely used as a method of insect pest management for several decades. In recent years, a transgenic corn expressing the Cry3Bb1 toxin has been successfully used for protection against corn rootworm larvae (Genus...

  11. NV Proteins of Fish Novirhabdovirus Recruit Cellular PPM1Bb Protein Phosphatase and Antagonize RIG-I-Mediated IFN Induction.

    PubMed

    Biacchesi, Stéphane; Mérour, Emilie; Chevret, Didier; Lamoureux, Annie; Bernard, Julie; Brémont, Michel

    2017-03-09

    Non virion (NV) protein expression is critical for fish Novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV), in vivo pathogenesis. However, the mechanism by which NV promotes the viral replication is still unclear. We developed an approach based on reverse genetics and interactomic and identified several NV-associated cellular partners underlying cellular pathways as potential viral targets. Among these cell partners, we showed that NV proteins specifically interact with a protein phosphatase, Mg(2+)/Mn(2+)-dependent, 1Bb (PPM1Bb) and recruit it in the close vicinity of mitochondria, a subcellular compartment important for retinoic acid-inducible gene-I- (RIG-I)-mediated interferon induction pathway. PPM1B proteins belong to the PP2C family of serine/threonine (Ser/Thr) protein phosphatase and have recently been shown to negatively regulate the host antiviral response via dephosphorylating Traf family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1). We demonstrated that NV proteins and PPM1Bb counteract RIG-I- and TBK1-dependent interferon (IFN) and IFN-stimulated gene promoter induction in fish cells and, hence, the establishment of an antiviral state. Furthermore, the expression of VHSV NV strongly reduced TBK1 phosphorylation and thus its activation. Our findings provide evidence for a previously undescribed mechanism by which a viral protein recruits PPM1Bb protein phosphatase to subvert innate immune recognition.

  12. Finished Genome Sequence of Bacillus cereus Strain 03BB87, a Clinical Isolate with B. anthracis Virulence Genes.

    PubMed

    Johnson, Shannon L; Minogue, Timothy D; Teshima, Hazuki; Davenport, Karen W; Shea, April A; Miner, Haven L; Wolcott, Mark J; Chain, Patrick S G

    2015-01-15

    Bacillus cereus strain 03BB87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. Here we present the finished genome sequence of that pathogen, including a 5.46-Mb chromosome and two plasmids (209 and 52 Kb, respectively).

  13. Preparation of biologically active platelet-derived growth factor type BB from a fusion protein expressed in Escherichia coli

    SciTech Connect

    Hoppe, J.; Weich, H.A.; Eichner, W. )

    1989-04-04

    Preparations of the mitogen platelet-derived growth factor (PDGF) from human platelets contain two related polypeptides termed A chain and B chain. PDGF-B is highly homologous to a portion of p28{sup v-sis}, the transforming protein of simian sarcoma virus. The authors have studied the mitogenic potential of a PDGF-BB-like homodimer by expressing the sequence coding for the mature part of PDGF-B in Escherichia coli. Expression was achieved as cro-{beta}-gal-PDGF-B fusion protein which was exclusively found in the inclusion bodies. A monomeric PDGF-B fragment shortened by 12 amino acid residues from the NH{sub 2} terminus was excised from the fusion protein by CNBr cleavage. After protection of thiols by S-sulfonation, this fragment was purified by gel permeation chromatography and reversed-phase high-performance liquid chromatography. This monomeric protein was dimerized in the presence of a mixture of reduced and oxidized glutathione to yield biologically active rPDGF-BB with an overall yield of {approx}0.7 mg of rPDGF-BB/L of culture. Escherichia coli rPDGF-BB stimulated ({sup 3}H)thymidine incorporation into AKR2B fibroblast at concentrations of about 1 ng/mL.

  14. Myricitrin inhibits PDGF-BB-stimulated vascular smooth muscle cell proliferation and migration through suppressing PDGFRβ/Akt/Erk signaling.

    PubMed

    Li, Jie; Zhang, Mei; Ma, Juanjuan

    2015-01-01

    Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) and the stimulation of platelet-derived growth factor (PDGF)-BB play major pathological processes involved in the development of cardiovascular diseases. As a result, the use of anti-proliferative and anti-migratory agents for VSMCs offers promise for the treatment of vascular disorders. Myricitrin is a naturally occurring phenolic compound which possesses antioxidant and anti-inflammatory activity. In this study, we investigate the inhibitory effect of myricitrin on PDGF-BB-induced VSMCs proliferation and migration. In accordance with these findings, myricitrin induced the arrest of cell cycle progression at G0/G1 phase. Myricitrin also decreased the expressions of G0/G1 specific regulatory proteins including cyclin D1, cyclin-dependent kinases (CDK) 4, cyclin E and CDK2, as well as increased the expression of p21 in PDGF-BB-induced VSMCs. Moreover, myricitrin inhibited PDGF-BB-induced phosphorylation of PDGFRβ, Akt and Erk1/2. These results suggest that myricitrin plays an important role in prevention of VSMCs proliferation and migration through the G0/G1 cell cycle arrest by PDGF signaling pathway. Thus, myricitrin is effective in reducing atherosclerotic process by blocking proliferation of VSMCs.

  15. Umbilical cord serum creatine kinase BB in the diagnosis of brain damage in the newborn: problems in interpretation.

    PubMed Central

    Kumpel, B; Wood, S M; Anthony, P P; Brimblecombe, F S

    1983-01-01

    Raised values of creatine kinase BB (CKBB) in umbilical cord serum were obtained with some normal babies and those with fetal distress. Further investigation showed that the umbilical artery and vein tissue contain high CKBB activity, indicating that some cord blood samples may not solely reflect CKBB liberated from the brain. PMID:6859922

  16. NV Proteins of Fish Novirhabdovirus Recruit Cellular PPM1Bb Protein Phosphatase and Antagonize RIG-I-Mediated IFN Induction

    PubMed Central

    Biacchesi, Stéphane; Mérour, Emilie; Chevret, Didier; Lamoureux, Annie; Bernard, Julie; Brémont, Michel

    2017-01-01

    Non virion (NV) protein expression is critical for fish Novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV), in vivo pathogenesis. However, the mechanism by which NV promotes the viral replication is still unclear. We developed an approach based on reverse genetics and interactomic and identified several NV-associated cellular partners underlying cellular pathways as potential viral targets. Among these cell partners, we showed that NV proteins specifically interact with a protein phosphatase, Mg2+/Mn2+-dependent, 1Bb (PPM1Bb) and recruit it in the close vicinity of mitochondria, a subcellular compartment important for retinoic acid-inducible gene-I- (RIG-I)-mediated interferon induction pathway. PPM1B proteins belong to the PP2C family of serine/threonine (Ser/Thr) protein phosphatase and have recently been shown to negatively regulate the host antiviral response via dephosphorylating Traf family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1). We demonstrated that NV proteins and PPM1Bb counteract RIG-I- and TBK1-dependent interferon (IFN) and IFN-stimulated gene promoter induction in fish cells and, hence, the establishment of an antiviral state. Furthermore, the expression of VHSV NV strongly reduced TBK1 phosphorylation and thus its activation. Our findings provide evidence for a previously undescribed mechanism by which a viral protein recruits PPM1Bb protein phosphatase to subvert innate immune recognition. PMID:28276468

  17. Greenhouse-selected resistance to Cry3Bb1-producing corn in three western corn rootworm populations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Transgenic corn producing the Bacillus thuringiensis (Bt) toxin Cry3Bb1 has been useful for controlling western corn rootworm, Diabrotica virgifera virgifera LeConte, one of the most economically important crop pests in the United States. However, rapid evolution of resistance by this beetle to Bt c...

  18. Finished Genome Sequence of Bacillus cereus Strain 03BB87, a Clinical Isolate with B. anthracis Virulence Genes

    DOE PAGES

    Johnson, Shannon L.; Minogue, Timothy D.; Teshima, Hazuki; ...

    2015-01-15

    Bacillus cereus strain 03BB87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. Here we present the finished genome sequence of that pathogen, including a 5.46-Mb chromosome and two plasmids (209 and 52 Kb, respectively).

  19. Assessment of fitness costs in Cry3Bb1 resistant and susceptible western corn rootworm (Coleoptera: Chrysomelidae) laboratory colonies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize production in the United States is dominated by plants genetically modified with transgenes from Bacillus thuringiensis (Bt). Varieties of Bt maize expressing Cry3Bb d endotoxins that specifically target corn rootworms (genus Diabrotica) have proven highly efficacious. However, development of ...

  20. 2008-09 Baccalaureate and beyond Longitudinal Study (B&B:08/09). First Look. NCES 2011-236

    ERIC Educational Resources Information Center

    Cataldi, Emily Forrest; Green, Caitlin; Henke, Robin; Lew, Terry; Woo, Jennie; Shepherd, Bryan; Siegel, Peter

    2011-01-01

    This "First Look" presents findings from the 2008-09 Baccalaureate and Beyond Longitudinal Study (B&B:08/09), which describes the enrollment and employment experiences, 1 year after they graduated, of a national sample of college graduates who completed a bachelor's degree in the 2007-08 academic year. The tables in this report…

  1. Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development

    PubMed Central

    Poulain, Morgane; Ober, Elke A.

    2011-01-01

    The vertebrate liver, pancreas and lung arise in close proximity from the multipotent foregut endoderm. Tissue-explant experiments uncovered instructive signals emanating from the neighbouring lateral plate mesoderm, directing the endoderm towards specific organ fates. This suggested that an intricate network of signals is required to control the specification and differentiation of each organ. Here, we show that sequential functions of Wnt2bb and Wnt2 control liver specification and proliferation in zebrafish. Their combined specific activities are essential for liver specification, as their loss of function causes liver agenesis. Conversely, excess wnt2bb or wnt2 induces ectopic liver tissue at the expense of pancreatic and anterior intestinal tissues, revealing the competence of intestinal endoderm to respond to hepatogenic signals. Epistasis experiments revealed that the receptor frizzled homolog 5 (fzd5) mediates part of the broader hepatic competence of the alimentary canal. fzd5 is required for early liver formation and interacts genetically with wnt2 as well as wnt2bb. In addition, lack of both ligands causes agenesis of the swim bladder, the structural homolog of the mammalian lung. Thus, tightly regulated spatiotemporal expression of wnt2bb, wnt2 and fzd5 is central to coordinating early liver, pancreas and swim bladder development from a multipotent foregut endoderm. PMID:21771809

  2. Different mechanisms influencing permeation of PDGF-AA and PDGF-BB across the blood-brain barrier.

    PubMed

    Kastin, Abba J; Akerstrom, Victoria; Hackler, Laszlo; Pan, Weihong

    2003-10-01

    Platelet-derived growth factor (PDGF) exerts neurotrophic and neuromodulatory effects on the CNS. To determine the permeability of the blood-brain barrier (BBB) to PDGF, we examined the blood-to-brain influx of radioactively labeled PDGF isoforms (PDGF-AA and PDGF-BB) by multiple-time regression analysis after intravenous (i.v.) injection and by in-situ perfusion, and also determined the physicochemical characteristics which affect their permeation across the BBB, including lipophilicity (measured by octanol:buffer partition coefficient), hydrogen bonding (measured by differences in octanol : buffer and isooctane : buffer partition coefficients), serum protein binding (measured by capillary electrophoresis), and stability of PDGF in blood 10 min after i.v. injection (measured by HPLC). After i.v. bolus injection, neither 125I-PDGF-AA nor 125I-PDGF-BB crossed the BBB, their influx rates being similar to that of the vascular marker 99mTc-albumin. 125I-PDGF-AA degraded significantly faster in blood than 125I-PDGF-BB. PDGF-BB, however, was completely bound to a large protein in serum whereas PDGF-AA showed no binding. Thus, degradation might explain the poor blood-to-brain influx of PDGF-AA, whereas protein binding could explain the poor influx of circulating PDGF-BB. Despite their lack of permeation in the intact mouse, both 125I-PDGF-AA and 125I-PDGF-BB entered the brain by perfusion in blood-free buffer, and the significantly faster rate of 125I-PDGF-AA than 125I-PDGF-BB may be explained by the lower hydrogen bonding potential of 125I-PDGF-AA. Thus, the lack of significant distribution of PDGF from blood to brain is not because of the intrinsic barrier function of the BBB but probably because of degradation and protein binding. Information from these studies could be useful in the design of analogues for delivery of PDGF as a therapeutic agent.

  3. Characterization of binding mode of action of a blocking anti-platelet-derived growth factor (PDGF)-B monoclonal antibody, MOR8457, reveals conformational flexibility and avidity needed for PDGF-BB to bind PDGF receptor-β.

    PubMed

    Kuai, Jun; Mosyak, Lidia; Brooks, Jon; Cain, Michael; Carven, Gregory J; Ogawa, Shinji; Ishino, Tetsuya; Tam, May; Lavallie, Edward R; Yang, Zhiyong; Ponsel, Dirk; Rauchenberger, Robert; Arch, Robert; Pullen, Nick

    2015-03-17

    Platelet derived growth factor-BB (PDGF-BB) is an important mitogen and cell survival factor during development. PDGF-BB binds PDGF receptor-β (PDGFRβ) to trigger receptor dimerization and tyrosine kinase activation. We present the pharmacological and biophysical characterization of a blocking PDGF-BB monoclonal antibody, MOR8457, and contrast this to PDGFRβ. MOR8457 binds to PDGF-BB with high affinity and selectivity, and prevents PDGF-BB induced cell proliferation competitively and with high potency. The structural characterization of the MOR8457-PDGF-BB complex indicates that MOR8457 binds with a 2:1 stoichiometry, but that binding of a single MOR8457 moiety is sufficient to prevent binding to PDGFRβ. Comparison of the MOR8457-PDGF-BB structure with that of the PDGFRβ-PDGF-BB complex suggested the potential reason for this was a substantial bending and twisting of PDGF-BB in the MOR8457 structure, relative to the structures of PDGF-BB alone, bound to a PDGF-BB aptamer or PDGFRβ, which makes it nonpermissive for PDGFRβ binding. These biochemical and structural data offer insights into the permissive structure of PDGF-BB needed for agonism as well as strategies for developing specific PDGF ligand antagonists.

  4. Platelet-Derived Growth Factor BB Enhances Osteogenesis of Adipose-Derived But Not Bone Marrow-Derived Mesenchymal Stromal/Stem Cells.

    PubMed

    Hung, Ben P; Hutton, Daphne L; Kozielski, Kristen L; Bishop, Corey J; Naved, Bilal; Green, Jordan J; Caplan, Arnold I; Gimble, Jeffrey M; Dorafshar, Amir H; Grayson, Warren L

    2015-09-01

    Tissue engineering using mesenchymal stem cells (MSCs) holds great promise for regenerating critically sized bone defects. While the bone marrow-derived MSC is the most widely studied stromal/stem cell type for this application, its rarity within bone marrow and painful isolation procedure have motivated investigation of alternative cell sources. Adipose-derived stromal/stem cells (ASCs) are more abundant and more easily procured; furthermore, they also possess robust osteogenic potency. While these two cell types are widely considered very similar, there is a growing appreciation of possible innate differences in their biology and response to growth factors. In particular, reports indicate that their osteogenic response to platelet-derived growth factor BB (PDGF-BB) is markedly different: MSCs responded negatively or not at all to PDGF-BB while ASCs exhibited enhanced mineralization in response to physiological concentrations of PDGF-BB. In this study, we directly tested whether a fundamental difference existed between the osteogenic responses of MSCs and ASCs to PDGF-BB. MSCs and ASCs cultured under identical osteogenic conditions responded disparately to 20 ng/ml of PDGF-BB: MSCs exhibited no difference in mineralization while ASCs produced more calcium per cell. siRNA-mediated knockdown of PDGFRβ within ASCs abolished their ability to respond to PDGF-BB. Gene expression was also different; MSCs generally downregulated and ASCs generally upregulated osteogenic genes in response to PDGF-BB. ASCs transduced to produce PDGF-BB resulted in more regenerated bone within a critically sized murine calvarial defect compared to control ASCs, indicating PDGF-BB used specifically in conjunction with ASCs might enhance tissue engineering approaches for bone regeneration.

  5. Bifidobacterium animalis subsp. lactis BB-12 administration in early childhood: a randomized clinical trial of effects on oral colonization by mutans streptococci and the probiotic.

    PubMed

    Taipale, T; Pienihäkkinen, K; Salminen, S; Jokela, J; Söderling, E

    2012-01-01

    A randomized clinical trial studied the effects of early administration of Bifidobacterium animalis subsp. lactis BB-12 (BB-12) on oral colonization of (1) mutans streptococci (MS), and (2) BB-12. In this double-blind, placebo-controlled study, infants (n = 106) received probiotic bacteria (BB-12 group), xylitol (X group), or sorbitol (S group). Test tablets were administered twice a day (from the age of 1-2 months) with a novel slow-release pacifier or a spoon (daily dose of BB-12 10(10) CFU, polyol 200-600 mg). Samples were collected from mucosa/teeth at the age of 8 months and 2 years for BB- 12 determination (qPCR) and plate culturing of MS (MSB, TYCSB), lactobacilli (Rogosa) and yeasts (Sabouraud). The MS levels of the mothers were determined (Dentocult SM Strip Mutans). The baseline characteristics of the three groups were similar. Mean duration of tablet delivery was 14.9 ± 6.7 months. In all groups, >90% of the mothers showed high MS counts (log CFU ≥5). MS colonization percentages of the children at the age of 2 years were rather low (BB-12 group: 6%; X group: 31%; S group: 10%; p < 0.05). The levels of lactobacilli and yeasts did not differ between the groups. BB-12 cell counts barely exceeding the detection limit were found in three of the oral samples of the 8-month-old children; however, the 2-year samples did not contain BB-12. The early administration of BB-12 did not result in permanent oral colonization of this probiotic or significantly affect MS colonization in the children.

  6. Cry3Bb1-Resistant Western Corn Rootworm, Diabrotica virgifera virgifera (LeConte) Does Not Exhibit Cross-Resistance to DvSnf7 dsRNA

    PubMed Central

    Khajuria, Chitvan; Pleau, Michael; Ilagan, Oliver; Chen, Mao; Jiang, Changjian; Price, Paula; McNulty, Brian; Clark, Thomas; Head, Graham

    2017-01-01

    Background and Methodology There is a continuing need to express new insect control compounds in transgenic maize against western corn rootworm, Diabrotica virgifera virgifera (LeConte) (WCR). In this study three experiments were conducted to determine cross-resistance between the new insecticidal DvSnf7 dsRNA, and Bacillus thuringiensis (Bt) Cry3Bb1; used to control WCR since 2003, with field-evolved resistance being reported. Laboratory susceptible and Cry3Bb1-resistant WCR were evaluated against DvSnf7 dsRNA in larval diet-incorporation bioassays. Additionally, the susceptibility of seven field and one field-derived WCR populations to DvSnf7 (and Cry3Bb1) was assessed in larval diet-overlay bioassays. Finally, beetle emergence of laboratory susceptible and Cry3Bb1-resistant WCR was evaluated with maize plants in the greenhouse expressing Cry3Bb1, Cry34Ab1/Cry35Ab1, or DvSnf7 dsRNA singly, or in combination. Principal Findings and Conclusions The Cry3Bb1-resistant colony had slight but significantly (2.7-fold; P<0.05) decreased susceptibility to DvSnf7 compared to the susceptible colony, but when repeated using a field-derived WCR population selected for reduced Cry3Bb1 susceptibility, there was no significant difference (P<0.05) in DvSnf7 susceptibility compared to that same susceptible population. Additionally, this 2.7-fold difference in susceptibility falls within the range of DvSnf7 susceptibility among the seven field populations tested. Additionally, there was no correlation between susceptibility to DvSnf7 and Cry3Bb1 for all populations evaluated. In greenhouse studies, there were no significant differences (P<0.05) between beetle emergence of susceptible and Cry3Bb1-resistant colonies on DvSnf7 and Cry34Ab1/Cry35Ab1, and between DvSnf7 and MON 87411 (DvSnf7 + Cry3Bb1) for the Cry3Bb1-resistant colony. These results demonstrate no cross-resistance between DvSnf7 and Cry3Bb1 against WCR. Therefore, pyramiding DvSnf7 with Bt proteins such as Cry3Bb1 and

  7. 4-1BB ligand enhances tumor-specific immunity of poxvirus vaccines

    PubMed Central

    Kudo-Saito, Chie; Hodge, James W.; Kwak, Heesun; Kim-Schulze, Seunghee; Schlom, Jeffrey; Kaufman, Howard L.

    2007-01-01

    Purpose Recombinant poxvirus vaccines have been explored as tumor vaccines. The immunogenicity of these vaccines can be enhanced by co-expressing costimulatory molecules and tumor-associated antigens. While the B7-CD28 interaction has been most comprehensively investigated, other costimulatory molecules utilize different signaling pathways and might provide further cooperation in T cell priming and survival. 4-1BB (CD137) is a TNF family member and is critical for activation and long-term maintenance of primed T-cells. This study was conducted to determine if a poxvirus expressing the ligand for 4-1BB (4-1BBL) could further improve the immune and therapeutic responses of a previously reported poxvirus vaccine expressing a triad of costimulatory molecules (B7.1, ICAM-1, and LFA-3). Experimental Design A recombinant vaccinia virus expressing 4-1BBL was generated and characterized in an in vitro infection system. This vaccine was then used alone or in combination with a vaccinia virus expressing CEA, B7.1, ICAM-1, and LFA-3 in CEA-transgenic mice bearing established MC38 tumors. Tumor growth and immune responses against CEA and other tumor-associated antigens were determined. The level of anti-apoptotic proteins in responding T cells was determined by flow cytometry on tetramer selected T cells. Results The combination of 4-1BBL with B7.1-based poxvirus vaccination resulted in significantly enhanced therapeutic effects against CEA-expressing tumors in a CEA transgenic mouse model. This was associated with an increased level of CEA-specific CD4+ and CD8+ T cell responses, induction of antigen spreading to p53 and gp70, increased accumulation of CEA-specific T cells in the tumor microenvironment, and increased expression of bcl-XL and bcl-2 in CD4+ and CD8+ T cells in vaccinated mice. Conclusion 4-1BBL cooperates with B7 in enhancing anti-tumor and immunologic responses using a recombinant poxvirus vaccine model. The inclusion of costimulatory molecules targeting

  8. TRPM7 channel regulates PDGF-BB-induced proliferation of hepatic stellate cells via PI3K and ERK pathways

    SciTech Connect

    Fang, Ling Zhan, Shuxiang; Huang, Cheng; Cheng, Xi; Lv, Xiongwen; Si, Hongfang; Li, Jun

    2013-11-01

    TRPM7, a non-selective cation channel of the TRP channel superfamily, is implicated in diverse physiological and pathological processes including cell proliferation. Recently, TRPM7 has been reported in hepatic stellate cells (HSCs). Here, we investigated the contribution role of TRPM7 in activated HSC-T6 cell (a rat hepatic stellate cell line) proliferation. TRPM7 mRNA and protein were measured by RT-PCR and Western blot in rat model of liver fibrosis in vivo and PDGF-BB-activated HSC-T6 cells in vitro. Both mRNA and protein of TRPM7 were dramatically increased in CCl{sub 4}-treated rat livers. Stimulation of HSC-T6 cells with PDGF-BB resulted in a time-dependent increase of TRPM7 mRNA and protein. However, PDGF-BB-induced HSC-T6 cell proliferation was inhibited by non-specific TRPM7 blocker 2-aminoethoxydiphenyl borate (2-APB) or synthetic siRNA targeting TRPM7, and this was accompanied by downregulation of cell cycle proteins, cyclin D1, PCNA and CDK4. Blockade of TRPM7 channels also attenuated PDGF-BB induced expression of myofibroblast markers as measured by the induction of α-SMA and Col1α1. Furthermore, the phosphorylation of ERK and AKT, associated with cell proliferation, decreased in TRPM7 deficient HSC-T6 cells. These observations suggested that TRPM7 channels contribute to perpetuated fibroblast activation and proliferation of PDGF-BB induced HSC-T6 cells via the activation of ERK and PI3K pathways. Therefore, TRPM7 may constitute a useful target for the treatment of liver fibrosis. - Highlights: • Upregulation of TRPM7 mRNA and protein in the fibrotic livers from CCl{sub 4}-treated rats. • Increasing expression of TRPM7 mRNA and protein during HSC activation. • Blockade of TRPM7 inhibited the PDGF-BB induced proliferation of HSC-T6 cells. • Blockade of TRPM7 decreased α-SMA and Col1α1 expressions in activated HSC-T6 cells. • TRPM7 up-regulation contributes to the activation of ERK and AKT pathways.

  9. Heightened clinical utility of smartphone versus body-worn inertial system for shoulder function B-B score

    PubMed Central

    Aminian, Kamiar; Ancey, Céline; Jaccard, Hervé; Lécureux, Estelle; Duc, Cyntia; Farron, Alain; Jolles, Brigitte M.; Gleeson, Nigel

    2017-01-01

    Background The B-B Score is a straightforward kinematic shoulder function score including only two movements (hand to the Back + lift hand as to change a Bulb) that demonstrated sound measurement properties for patients for various shoulder pathologies. However, the B-B Score results using a smartphone or a reference system have not yet been compared. Provided that the measurement properties are comparable, the use of a smartphone would offer substantial practical advantages. This study investigated the concurrent validity of a smartphone and a reference inertial system for the measurement of the kinematic shoulder function B-B Score. Methods Sixty-five patients with shoulder conditions (with rotator cuff conditions, adhesive capsulitis and proximal humerus fracture) and 20 healthy participants were evaluated using a smartphone and a reference inertial system. Measurements were performed twice, alternating between two evaluators. The B-B Score differences between groups, differences between devices, relationship between devices, intra- and inter-evaluator reproducibility were analysed. Results The smartphone mean scores (SD) were 94.1 (11.1) for controls and 54.1 (18.3) for patients (P < 0.01). The difference between devices was non-significant for the control (P = 0.16) and the patient group (P = 0.81). The analysis of the relationship between devices showed 0.97 ICC, −0.6 bias and −13.2 to 12.0 limits of agreement (LOA). The smartphone intra-evaluator ICC was 0.92, the bias 1.5 and the LOA −17.4 to 20.3. The smartphone inter-evaluator ICC was 0.92, the bias 1.5 and the LOA −16.9 to 20.0. Conclusions The B-B Score results measured with a smartphone were comparable to those of an inertial system. While single measurements diverged in some cases, the intra- and inter-evaluator reproducibility was excellent and was equivalent between devices. The B-B score measured with a smartphone is straightforward and as efficient as a reference inertial system

  10. Recombinant human platelet-derived growth factor-BB versus autologous bone graft in foot and ankle fusion: A systematic review and meta-analysis.

    PubMed

    Sun, Han; Lu, Pei-Pei; Zhou, Ping-Hui; Sun, Si-Wei; Zhang, Hong-Tao; Liu, Yi-Jie; Yang, Xu; Shen, Xiao-Feng; Yang, Hui-Lin

    2017-03-01

    Today, autogenous bone graft (ABG) is still considered as the gold standard for joint fusion. Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) which is of chemotactic and mitogenic to mesenchymal stem cells and possesses outstanding osteogenetic potentials has been used for ankle and foot fusion in recent years. The goal of this article is to evaluate the safety and efficacy of rhPDGF-BB versus ABG in foot and ankle fusion. The PubMed MEDLINE, EMBASE, Web of Science, and Cochrane Library were systematic searched. Finally, three randomized controlled trials (RCTs) with 634 patients were enrolled in this study. Results of radiologic effectiveness which included CT and radiographic union rates revealed that there was no significant difference between rhPDGF-BB approach and ABG approach. Analysis of clinical results held the same outcomes expect that ABG group was superior in long-term Short Form-12 physical component scores. The pooled results also demonstrated that rhPDGF-BB was as safe as ABG in foot and ankle surgery. However, autograft harvesting procedure has some drawbacks such as donor-site pain and morbidity, additional operation time, blood loss, and scarring, which can be overcome by rhPDGF-BB. Thus, rhPDGF-BB is a viable alternative to autograft in foot and ankle fusion surgery. Yet, more high-quality RCTs with long-term follow-up are still required to make the final conclusion.

  11. Crystal structure of LpxC from Pseudomonas aeruginosa complexed with the potent BB-78485 inhibitor

    SciTech Connect

    Mochalkin, Igor; Knafels, John D.; Lightle, Sandra

    2008-04-02

    The cell wall in Gram-negative bacteria is surrounded by an outer membrane comprised of charged lipopolysaccharide (LPS) molecules that prevent entry of hydrophobic agents into the cell and protect the bacterium from many antibiotics. The hydrophobic anchor of LPS is lipid A, the biosynthesis of which is essential for bacterial growth and viability. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) is an essential zinc-dependant enzyme that catalyzes the conversion of UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)glucosamine and acetate in the biosynthesis of lipid A, and for this reason, LpxC is an attractive target for antibacterial drug discovery. Here we disclose a 1.9 A resolution crystal structure of LpxC from Pseudomonas aeruginosa (paLpxC) in a complex with the potent BB-78485 inhibitor. To our knowledge, this is the first crystal structure of LpxC with a small-molecule inhibitor that shows antibacterial activity against a wide range of Gram-negative pathogens. Accordingly, this structure can provide important information for lead optimization and rational design of the effective small-molecule LpxC inhibitors for successful treatment of Gram-negative infections.

  12. Evidence that BosR (BB0647) Is a Positive Autoregulator in Borrelia burgdorferi

    PubMed Central

    Ouyang, Zhiming; Zhou, Jianli

    2016-01-01

    Borrelia burgdorferi survives in nature through a complex tick-mammalian life cycle. During its transit between ticks and mammalian hosts, B. burgdorferi must dramatically alter its outer surface profile in order to interact with and adapt to these two diverse niches. It has been established that the regulator BosR (BB0647) in B. burgdorferi plays important roles in modulating borrelial host adaptation. However, to date, how bosR expression itself is controlled in B. burgdorferi remains largely unknown. Previously, it has been shown that DNA sequences upstream of BosR harbor multiple sites for the binding of recombinant BosR, suggesting that BosR may influence its own expression in B. burgdorferi. However, direct experimental evidence supporting this putative autoregulation of BosR has been lacking. Here, we investigated the expression of bosR throughout the tick-mammal life cycle of B. burgdorferi via quantitative reverse transcription (RT)-PCR analyses. Our data indicated that bosR is expressed not only during mouse infection, but also during the tick acquisition, intermolt, and transmission phases. Further investigation revealed that bosR expression in B. burgdorferi is influenced by environmental stimuli, such as temperature shift and pH change. By employing luciferase reporter assays, we also identified two promoters potentially driving bosR transcription. Our study offers strong support for the long-postulated function of BosR as an autoregulator in B. burgdorferi. PMID:27324485

  13. Comparative Physical Mapping Between Oryza sativa (AA Genome Type) and O. punctata (BB Genome Type)

    PubMed Central

    Kim, HyeRan; Miguel, Phillip San; Nelson, William; Collura, Kristi; Wissotski, Marina; Walling, Jason G.; Kim, Jun Pyo; Jackson, Scott A.; Soderlund, Carol; Wing, Rod A.

    2007-01-01

    A comparative physical map of the AA genome (Oryza sativa) and the BB genome (O. punctata) was constructed by aligning a physical map of O. punctata, deduced from 63,942 BAC end sequences (BESs) and 34,224 fingerprints, onto the O. sativa genome sequence. The level of conservation of each chromosome between the two species was determined by calculating a ratio of BES alignments. The alignment result suggests more divergence of intergenic and repeat regions in comparison to gene-rich regions. Further, this characteristic enabled localization of heterochromatic and euchromatic regions for each chromosome of both species. The alignment identified 16 locations containing expansions, contractions, inversions, and transpositions. By aligning 40% of the punctata BES on the map, 87% of the punctata FPC map covered 98% of the O. sativa genome sequence. The genome size of O. punctata was estimated to be 8% larger than that of O. sativa with individual chromosome differences of 1.5–16.5%. The sum of expansions and contractions observed in regions >500 kb were similar, suggesting that most of the contractions/expansions contributing to the genome size difference between the two species are small, thus preserving the macro-collinearity between these species, which diverged ∼2 million years ago. PMID:17339227

  14. Supercritical carbon dioxide interpolymer complexes improve survival of B. longum Bb-46 in simulated gastrointestinal fluids.

    PubMed

    Thantsha, Mapitsi S; Cloete, Thomas E; Moolman, Francis S; Labuschagne, Philip W

    2009-01-31

    Gastric acidity is the main factor affecting viability of probiotics in the gastrointestinal tract (GIT). This study investigated the survival in simulated gastrointestinal fluids of Bifidobacterium longum Bb-46 encapsulated in interpolymer complexes formed in supercritical carbon dioxide (scCO(2)). Bacteria were exposed sequentially to simulated gastric fluid (SGF, pH 2) for 2 h and simulated intestinal fluid (SIF, pH 6.8) for 6 or 24 h. Total encapsulated bacteria were determined by suspending 1 g of product in SIF for 6 h at 37 degrees C prior to plating out. Plates were incubated anaerobically at 37 degrees C for 72 h. The interpolymer complex displayed pH-responsive release properties, with little to no release in SGF and substantial release in SIF. There was a limited reduction in viable counts at the end of exposure period due to encapsulation. Protection efficiency of the interpolymer complex was improved by addition of glyceryl monostearate (GMS). Gelatine capsules delayed release of bacteria from the interpolymer complex thus minimizing time of exposure to the detrimental conditions. Use of poly(caprolactone) (PCL), ethylene oxide-propylene oxide triblock copolymer (PEO-PPO-PEO) decreased the protection efficiency of the matrix. Interpolymer complex encapsulation showed potential for protection of probiotics and therefore for application in food and pharmaceuticals.

  15. Decreased catecholamine secretion from the adrenal medullae of chronically diabetic BB-Wistar rats

    NASA Technical Reports Server (NTRS)

    Wilke, R. A.; Riley, D. A.; Lelkes, P. I.; Hillard, C. J.

    1993-01-01

    Many humans with IDDM eventually lose the capacity to secrete epinephrine from their adrenal medullae. The mechanism for this pathological change is unknown. We hypothesized that this abnormality is attributable to neuropathic changes in the greater splanchnic nerves or in the chromaffin cells that they innervate. To study this hypothesis, we isolated rat adrenal glands, perfused them ex vivo, and measured the epinephrine content of the perfusate under various conditions of stimulation. We used transmural electrical stimulation (20-80 V, at 10 Hz) to induce epinephrine secretion indirectly by selectively activating residual splanchnic nerve terminals within the isolated glands. Under these conditions, epinephrine secretion was severely attenuated in glands from female BB-Wistar rats with diabetes of 4 mo duration compared with their age-matched, nondiabetic controls. These perfused diabetic adrenal medullae also demonstrated decreased catecholamine release in response to direct chromaffin cell depolarization with 20 mM K+, evidence that a functional alteration exists within the chromaffin cells themselves. Nonetheless, total catecholamine content of adrenal medullae from these diabetic rats was not significantly different from controls, indicating that the secretory defect was not simply attributable to a difference in the amount of catecholamines stored and available for release. Herein, we also provide histological evidence of degenerative changes within the cholinergic nerve terminals that innervate these glands.

  16. Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study.

    PubMed

    Merenstein, Daniel J; Tan, Tina P; Molokin, Aleksey; Smith, Keisha Herbin; Roberts, Robert F; Shara, Nawar M; Mete, Mihriye; Sanders, Mary Ellen; Solano-Aguilar, Gloria

    2015-01-01

    Probiotics are live microorganisms that, when administered in sufficient doses, provide health benefits on the host. The United States Food and Drug Administration (FDA) requires phase I safety studies for probiotics when the intended use of the product is as a drug. The purpose of the study was to determine the safety of Bifidobacterium animalis subsp lactis (B. lactis) strain BB-12 (BB-12)-supplemented yogurt when consumed by a generally healthy group of adults who were prescribed a 10-day course of antibiotics for a respiratory infection. Secondary aims were to assess the ability of BB-12 to affect the expression of whole blood immune markers associated with cell activation and inflammatory response. A phase I, double-blinded, randomized controlled study was conducted in compliance with FDA guidelines for an Investigational New Drug (IND). Forty participants were randomly assigned to consume 4 ounces of either BB-12 -supplemented yogurt or non-supplemented control yogurt daily for 10 d. The primary outcome was to assess safety and tolerability, assessed by the number of reported adverse events. A total of 165 non-serious adverse events were reported, with no differences between the control and BB-12 groups. When compared to the control group, B lactis fecal levels were modestly higher in the BB-12-supplemented group. In a small subset of patients, changes in whole blood expression of genes associated with regulation and activation of immune cells were detected in the BB-12-supplemented group. BB-12-supplemented yogurt is safe and well tolerated when consumed by healthy adults concurrently taking antibiotics. This study will form the basis for future randomized clinical trials investigating the potential immunomodulatory effects of BB-12-supplemented yogurt in a variety of disease states.

  17. Search for the standard model Higgs Boson in the pp[over]-->ZH-->nunu[over]bb[over] channel.

    PubMed

    Abazov, V M; Abbott, B; Abolins, M; Acharya, B S; Adams, M; Adams, T; Agelou, M; Agram, J-L; Ahn, S H; Ahsan, M; Alexeev, G D; Alkhazov, G; Alton, A; Alverson, G; Alves, G A; Anastasoaie, M; Andeen, T; Anderson, S; Andrieu, B; Anzelc, M S; Arnoud, Y; Arov, M; Askew, A; Asman, B; Jesus, A C S Assis; Atramentov, O; Autermann, C; Avila, C; Ay, C; Badaud, F; Baden, A; Bagby, L; Baldin, B; Bandurin, D V; Banerjee, P; Banerjee, S; Barberis, E; Bargassa, P; Baringer, P; Barnes, C; Barreto, J; Bartlett, J F; Bassler, U; Bauer, D; Bean, A; Begalli, M; Begel, M; Belanger-Champagne, C; Bellantoni, L; Bellavance, A; Benitez, J A; Beri, S B; Bernardi, G; Bernhard, R; Berntzon, L; Bertram, I; Besançon, M; Beuselinck, R; Bezzubov, V A; Bhat, P C; Bhatnagar, V; Binder, M; Biscarat, C; Black, K M; Blackler, I; Blazey, G; Blekman, F; Blessing, S; Bloch, D; Bloom, K; Blumenschein, U; Boehnlein, A; Boeriu, O; Bolton, T A; Borissov, G; Bos, K; Bose, T; Brandt, A; Brock, R; Brooijmans, G; Bross, A; Brown, D; Buchanan, N J; Buchholz, D; Buehler, M; Buescher, V; Burdin, S; Burke, S; Burnett, T H; Busato, E; Buszello, C P; Butler, J M; Calfayan, P; Calvet, S; Cammin, J; Caron, S; Carvalho, W; Casey, B C K; Cason, N M; Castilla-Valdez, H; Chakrabarti, S; Chakraborty, D; Chan, K M; Chandra, A; Chapin, D; Charles, F; Cheu, E; Chevallier, F; Cho, D K; Choi, S; Choudhary, B; Christofek, L; Claes, D; Clément, B; Clément, C; Coadou, Y; Cooke, M; Cooper, W E; Coppage, D; Corcoran, M; Cousinou, M-C; Cox, B; Crépé-Renaudin, S; Cutts, D; Cwiok, M; da Motta, H; Das, A; Das, M; Davies, B; Davies, G; Davis, G A; De, K; de Jong, P; de Jong, S J; Cruz-Burelo, E De La; Martins, C De Oliveira; Degenhardt, J D; Déliot, F; Demarteau, M; Demina, R; Demine, P; Denisov, D; Denisov, S P; Desai, S; Diehl, H T; Diesburg, M; Doidge, M; Dominguez, A; Dong, H; Dudko, L V; Duflot, L; Dugad, S R; Duperrin, A; Dyer, J; Dyshkant, A; Eads, M; Edmunds, D; Edwards, T; Ellison, J; Elmsheuser, J; Elvira, V D; Eno, S; Ermolov, P; Estrada, J; Evans, H; Evdokimov, A; Evdokimov, V N; Fatakia, S N; Feligioni, L; Ferapontov, A V; Ferbel, T; Fiedler, F; Filthaut, F; Fisher, W; Fisk, H E; Fleck, I; Ford, M; Fortner, M; Fox, H; Fu, S; Fuess, S; Gadfort, T; Galea, C F; Gallas, E; Galyaev, E; Garcia, C; Garcia-Bellido, A; Gardner, J; Gavrilov, V; Gay, A; Gay, P; Gelé, D; Gelhaus, R; Gerber, C E; Gershtein, Y; Gillberg, D; Ginther, G; Gollub, N; Gómez, B; Goussiou, A; Grannis, P D; Greenlee, H; Greenwood, Z D; Gregores, E M; Grenier, G; Gris, Ph; Grivaz, J-F; Grünendahl, S; Grünewald, M W; Guo, F; Guo, J; Gutierrez, G; Gutierrez, P; Haas, A; Hadley, N J; Haefner, P; Hagopian, S; Haley, J; Hall, I; Hall, R E; Han, L; Hanagaki, K; Harder, K; Harel, A; Harrington, R; Hauptman, J M; Hauser, R; Hays, J; Hebbeker, T; Hedin, D; Hegeman, J G; Heinmiller, J M; Heinson, A P; Heintz, U; Hensel, C; Hesketh, G; Hildreth, M D; Hirosky, R; Hobbs, J D; Hoeneisen, B; Hoeth, H; Hohlfeld, M; Hong, S J; Hooper, R; Houben, P; Hu, Y; Hubacek, Z; Hynek, V; Iashvili, I; Illingworth, R; Ito, A S; Jabeen, S; Jaffré, M; Jain, S; Jakobs, K; Jarvis, C; Jenkins, A; Jesik, R; Johns, K; Johnson, C; Johnson, M; Jonckheere, A; Jonsson, P; Juste, A; Käfer, D; Kahn, S; Kajfasz, E; Kalinin, A M; Kalk, J M; Kalk, J R; Kappler, S; Karmanov, D; Kasper, J; Kasper, P; Katsanos, I; Kau, D; Kaur, R; Kehoe, R; Kermiche, S; Kesisoglou, S; Khalatyan, N; Khanov, A; Kharchilava, A; Kharzheev, Y M; Khatidze, D; Kim, H; Kim, T J; Kirby, M H; Klima, B; Kohli, J M; Konrath, J-P; Kopal, M; Korablev, V M; Kotcher, J; Kothari, B; Koubarovsky, A; Kozelov, A V; Kozminski, J; Krop, D; Kryemadhi, A; Kuhl, T; Kumar, A; Kunori, S; Kupco, A; Kurca, T; Kvita, J; Lager, S; Lammers, S; Landsberg, G; Lazoflores, J; Bihan, A-C Le; Lebrun, P; Lee, W M; Leflat, A; Lehner, F; Lesne, V; Leveque, J; Lewis, P; Li, J; Li, Q Z; Lima, J G R; Lincoln, D; Linnemann, J; Lipaev, V V; Lipton, R; Liu, Z; Lobo, L; Lobodenko, A; Lokajicek, M; Lounis, A; Love, P; Lubatti, H J; Lynker, M; Lyon, A L; Maciel, A K A; Madaras, R J; Mättig, P; Magass, C; Magerkurth, A; Magnan, A-M; Makovec, N; Mal, P K; Malbouisson, H B; Malik, S; Malyshev, V L; Mao, H S; Maravin, Y; Martens, M; Mattingly, S E K; McCarthy, R; Meder, D; Melnitchouk, A; Mendes, A; Mendoza, L; Merkin, M; Merritt, K W; Meyer, A; Meyer, J; Michaut, M; Miettinen, H; Millet, T; Mitrevski, J; Molina, J; Mondal, N K; Monk, J; Moore, R W; Moulik, T; Muanza, G S; Mulders, M; Mulhearn, M; Mundim, L; Mutaf, Y D; Nagy, E; Naimuddin, M; Narain, M; Naumann, N A; Neal, H A; Negret, J P; Nelson, S; Neustroev, P; Noeding, C; Nomerotski, A; Novaes, S F; Nunnemann, T; O'dell, V; O'neil, D C; Obrant, G; Oguri, V; Oliveira, N; Oshima, N; Otec, R; Y Garzón, G J Otero; Owen, M; Padley, P; Parashar, N; Park, S-J; Park, S K; Parsons, J; Partridge, R; Parua, N; Patwa, A; Pawloski, G; Perea, P M; Perez, E; Peters, K; Pétroff, P; Petteni, M; Piegaia, R; Pleier, M-A; Podesta-Lerma, P L M; Podstavkov, V M; Pogorelov, Y; Pol, M-E; Pompos, A; Pope, B G; Popov, A V; da Silva, W L Prado; Prosper, H B; Protopopescu, S; Qian, J; Quadt, A; Quinn, B; Rani, K J; Ranjan, K; Ratoff, P N; Renkel, P; Reucroft, S; Rijssenbeek, M; Ripp-Baudot, I; Rizatdinova, F; Robinson, S; Rodrigues, R F; Royon, C; Rubinov, P; Ruchti, R; Rud, V I; Sajot, G; Sánchez-Hernández, A; Sanders, M P; Santoro, A; Savage, G; Sawyer, L; Scanlon, T; Schaile, D; Schamberger, R D; Scheglov, Y; Schellman, H; Schieferdecker, P; Schmitt, C; Schwanenberger, C; Schwartzman, A; Schwienhorst, R; Sengupta, S; Severini, H; Shabalina, E; Shamim, M; Shary, V; Shchukin, A A; Shephard, W D; Shivpuri, R K; Shpakov, D; Siccardi, V; Sidwell, R A; Simak, V; Sirotenko, V; Skubic, P; Slattery, P; Smith, R P; Snow, G R; Snow, J; Snyder, S; Söldner-Rembold, S; Song, X; Sonnenschein, L; Sopczak, A; Sosebee, M; Soustruznik, K; Souza, M; Spurlock, B; Stark, J; Steele, J; Stolin, V; Stone, A; Stoyanova, D A; Strandberg, J; Strang, M A; Strauss, M; Ströhmer, R; Strom, D; Strovink, M; Stutte, L; Sumowidagdo, S; Sznajder, A; Talby, M; Tamburello, P; Taylor, W; Telford, P; Temple, J; Tiller, B; Titov, M; Tokmenin, V V; Tomoto, M; Toole, T; Torchiani, I; Towers, S; Trefzger, T; Trincaz-Duvoid, S; Tsybychev, D; Tuchming, B; Tully, C; Turcot, A S; Tuts, P M; Unalan, R; Uvarov, L; Uvarov, S; Uzunyan, S; Vachon, B; van den Berg, P J; Kooten, R Van; van Leeuwen, W M; Varelas, N; Varnes, E W; Vartapetian, A; Vasilyev, I A; Vaupel, M; Verdier, P; Vertogradov, L S; Verzocchi, M; Villeneuve-Seguier, F; Vint, P; Vlimant, J-R; Toerne, E Von; Voutilainen, M; Vreeswijk, M; Wahl, H D; Wang, L; Warchol, J; Watts, G; Wayne, M; Weber, M; Weerts, H; Wermes, N; Wetstein, M; White, A; Wicke, D; Wilson, G W; Wimpenny, S J; Wobisch, M; Womersley, J; Wood, D R; Wyatt, T R; Xie, Y; Xuan, N; Yacoob, S; Yamada, R; Yan, M; Yasuda, T; Yatsunenko, Y A; Yip, K; Yoo, H D; Youn, S W; Yu, C; Yu, J; Yurkewicz, A; Zatserklyaniy, A; Zeitnitz, C; Zhang, D; Zhao, T; Zhou, B; Zhu, J; Zielinski, M; Zieminska, D; Zieminski, A; Zutshi, V; Zverev, E G

    2006-10-20

    We report a search for the standard model (SM) Higgs boson based on data collected by the D0 experiment at the Fermilab Tevatron Collider, corresponding to an integrated luminosity of 260 pb(-1). We study events with missing transverse energy and two acoplanar b jets, which provide sensitivity to the ZH production cross section in the nunu[over]bb[over] channel, and to WH production when the lepton from the W-->lnu decay is undetected. The data are consistent with the SM background expectation, and we set 95% C.L. upper limits on sigma(pp[over]-->ZH/WH)xB(H-->bb[over]) from 3.4/8.3 to 2.5/6.3 pb, for Higgs-boson masses between 105 and 135 GeV.

  18. Comparing conventional and supercritical extraction of (-)-mammea A/BB and the antioxidant activity of Calophyllum brasiliense extracts.

    PubMed

    Gonçalves, Renata Menoci; Lemos, Caroline Ortega Terra; Leal, Ivana Correa Ramos; Nakamura, Celso Vataru; Cortez, Diógenes Aparício Garcia; da Silva, Edson Antonio; Cabral, Vladimir Ferreira; Cardozo-Filho, Lúcio

    2013-05-24

    Calophyllum brasiliense is a rich source of bioactive coumarins, xanthones and biflavonoids. The aim of the study was to compare the phenol contents and the antioxidant activity of C. brasiliense extracts obtained by conventional and supercritical fluid extraction (SFE) methods, as well as the quantification of crude extracts and (-)-mammea A/BB yields. Dichloromethane and hexane were used as solvents for the conventional extractions and SFE was developed using supercritical CO₂; the kinetic curves were modeled using a second-order empirical model. The dichloromethane extract presented the best total yield, although it showed the lowest content of (-)-mammea A/BB. The concentration of the coumarin was considerably higher in extracts obtained by the supercritical fluid method and a higher antioxidant activity was assigned to extracts obtained by this technique. Concerning the total phenolic contents, both the dichloro-methane and the supercritical extractions produced satisfactory amounts. The SFE method proved to be more promising than conventional methods.

  19. Advanced Manufacturing Process for Lower Cost Rechargeable Lithium-ion Batteries for DOD Including the BB2590

    DTIC Science & Technology

    2013-11-30

    Nickelate in 18650 Cell 24 8. Installation of Resistance Welder 25 9. Bi-Cell Vacuum Dryer and with Activation Box 26 10. Semi...DOD lithium-ion rechargeable cells/batteries are composed of combinations using Asian 18650 cells including the BB2590. This dependence is due to the...much lower cost of the Asian and particularly the Chinese 18650 cells which are made on very large scale and also with lower labor costs. LithChem

  20. A luminol electrochemiluminescence aptasensor based on glucose oxidase modified gold nanoparticles for measurement of platelet-derived growth factor BB.

    PubMed

    Zhang, Jing-Jing; Cao, Jun-Tao; Shi, Gui-Fang; Huang, Ke-Jing; Liu, Yan-Ming; Ren, Shu-Wei

    2015-01-01

    A sandwich-type luminol electrochemiluminescence (ECL) aptasensor for highly sensitive and selective detection of platelet-derived growth factor BB (PDGF-BB) is fabricated. For this proposed ECL aptasensor, a multilayered AuNPs-electrochemically reduced graphene (AuNPs-EG) nanocomposite film was formed on the GCE surface as the base of the aptasensor through a co-electrodeposition method. The AuNPs-EG composites possess high conductivity to promote the electron transfer at the electrode interface and good biocompatibility and large surface area to capture large amounts of primary aptamer (Apt1), thus amplifying the detection response. Moreover, glucose oxidase (GOD) functionalized AuNPs labeled secondary aptamer (GOD-Apt2-AuNPs) was designed as the signal probe for the sandwiched aptasensor. Enhanced sensitivity was obtained by in situ generation of H2O2 from reaction between GOD and glucose and the excellent catalytic behavior of AuNPs to the ECL of the luminol-H2O2 system. Under the optimal conditions, the as-prepared ECL aptasensor exhibited excellent analytical property for the detection of PDGF-BB in the range from 1.0×10(-13) to 5.0×10(-10) mol L(-1) with a detection limit of 1.7×10(-14) mol L(-1) (S/N=3). The application of the present protocol was demonstrated by analyzing PDGF-BB in human serum and human urine samples with the recoveries from 85.0% to 110%.

  1. A recombination outside the BB deletion refines the location of the X-linked retinitis pigmentosa locus RP3

    SciTech Connect

    Fujita, R.; Bingham, E.; Forsythe, P.; McHenry, C.

    1996-07-01

    Genetic loci for X-linked retinitis pigmentosa (XLRP) have been mapped between Xp11.22 and Xp22.13 (RP2, RP3, RP6, and RP15). The RP3 gene, which is responsible for the predominant form of XLRP in most Caucasian populations, has been localized to Xp21.1 by linkage analysis and the map positions of chromosomal deletions associated with the disease. Previous linkage studies have suggested that RP3 is flanked by the markers DXS1110 (distal) and OTC (proximal). Patient BB was though to have RP because of a lesion at the RP3 locus, in addition to chronic granulomatous disease, Duchenne muscular dystrophy (DMD), mild mental retardation, and the McLeod phenotype. This patient carried a deletion extending {approximately}3 Mb from DMD in Xp21.3 to Xp21.1, with the proximal breakpoint located {approximately}40 kb centromeric to DXS1110. The RP3 gene, therefore, is believed to reside between DXS1110 and the proximal breakpoint of the BB deletion. In order to refine the location of RP3 and to ascertain patients with RP3, we have been analyzing several XLRP families for linkage to Xp markers. Linkage analysis in an American family of 27 individuals demonstrates segregation of XLRP with markers in Xp21.1, consistent with the RP3 subtype. One affected male shows a recombination event proximal to DXS1110. Additional markers within the DXS1110-OTC interval show that the crossover is between two novel polymorphic markers, DXS8349 and M6, both of which are present in BB DNA and lie centromeric to the proximal breakpoint. This recombination places the XLRP mutation in this family outside the BB deletion and redefines the location of RP3. 22 refs., 3 figs., 2 tabs.

  2. Vibrational spectral signatures of crystalline cellulose using high resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS)

    DOE PAGES

    Zhang, Libing; Lu, Zhou; Velarde, Luis; ...

    2015-03-03

    Both the C–H and O–H region spectra of crystalline cellulose were studied using the sub-wavenumber high-resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS) for the first time. The resolution of HR-BB-SFG-VS is about 10-times better than conventional scanning SFG-VS and has the capability of measuring the intrinsic spectral lineshape and revealing many more spectral details. With HR-BB-SFG-VS, we found that in cellulose samples from different sources, including Avicel and cellulose crystals isolated from algae Valonia (Iα) and tunicates (Iβ), the spectral signatures in the O–H region were unique for the two allomorphs, i.e. Iα and Iβ, while the spectral signaturesmore » in the C–H regions varied in all samples examined. Even though the origin of the different spectral signatures of the crystalline cellulose in the O–H and C–H vibrational frequency regions are yet to be correlated to the structure of cellulose, these results lead to new spectroscopic methods and opportunities to classify and to understand the basic crystalline structures, as well as variations in polymorphism of the crystalline cellulose.« less

  3. Vibrational Spectral Signatures of Crystalline Cellulose Using High Resolution Broadband Sum Frequency Generation Vibrational Spectroscopy (HR-BB-SFG-VS)

    SciTech Connect

    Zhang, Libing; Lu, Zhou; Velarde Ruiz Esparza, Luis A.; Fu, Li; Pu, Yunqiao; Ding, Shi-You; Ragauskas, Art J.; Wang, Hongfei; Yang, Bin

    2015-03-03

    Here we reported the first sub-wavenumber high-resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS) study on both the C-H and O-H region spectra of crystalline cellulose. HR-BB-SFG-VS has about 10 times better resolution than the conventional scanning SFG-VS and is known to be able to measure the intrinsic spectral lineshape and to resolve much more spectral details. With HR-BB-SFG-VS, we found that in cellulose from different sources, including Avicel and cellulose crystals isolated from algae Valonia (Iα) and tunicates (Iβ), the spectral signatures in the OH regions were unique for different allomorphs, i.e. Iα and Iβ, while the spectral signatures in the C-H regions varied in all samples examined. Even though the origin of the different behaviors of the crystalline cellulose in the O-H and C-H vibrational frequency regions is yet to be correlated to the structure of cellulose, these results provided new spectroscopic methods and opportunities to classify and understand the basic crystalline structure, as well as variations, in polymorphism of the crystalline cellulose structure.

  4. Hybrid CATV/MMW/BB lightwave transmission system based on fiber-wired/fiber-wireless/fiber-VLLC integrations.

    PubMed

    Li, Chung-Yi; Lu, Hai-Han; Lu, Ting-Chieh; Chu, Chien-An; Chen, Bo-Rui; Lin, Chun-Yu; Peng, Peng-Chun

    2015-12-14

    A hybrid lightwave transmission system for cable television (CATV)/millimeter-wave (MMW)/baseband (BB) signal transmission based on fiber-wired/fiber-wireless/fiber-visible laser light communication (VLLC) integrations is proposed and demonstrated. For down-link transmission, the light is intensity-modulated with 50-550 MHz CATV signal and optically promoted from 25 GHz radio frequency (RF) signal to 10 Gbps/50 GHz and 20 Gbps/100 GHz MMW data signals based on fiber-wired and fiber-wireless integrations. Good performances of carrier-to-noise ratio (CNR), composite second-order (CSO), composite triple-beat (CTB), and bit error rate (BER) are obtained over a 40-km single-mode fiber (SMF) and a 10-m RF wireless transport. For up-link transmission, the light is successfully intensity-remodulated with 5-Gbps BB data stream based on fiber-VLLC integration. Good BER performance is achieved over a 40-km SMF and a 10-m free-space VLLC transport. Such a hybrid CATV/MMW/BB lightwave transmission system is an attractive alternative, it gives the benefits of a communication link for broader bandwidth and higher transmission rate.

  5. Vibrational spectral signatures of crystalline cellulose using high resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS)

    SciTech Connect

    Zhang, Libing; Lu, Zhou; Velarde, Luis; Fu, Li; Pu, Yunqiao; Ding, Shi-You; Ragauskas, Arthur; Wang, Hong-Fei; Yang, Bin

    2015-03-03

    Both the C–H and O–H region spectra of crystalline cellulose were studied using the sub-wavenumber high-resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS) for the first time. The resolution of HR-BB-SFG-VS is about 10-times better than conventional scanning SFG-VS and has the capability of measuring the intrinsic spectral lineshape and revealing many more spectral details. With HR-BB-SFG-VS, we found that in cellulose samples from different sources, including Avicel and cellulose crystals isolated from algae Valonia (Iα) and tunicates (Iβ), the spectral signatures in the O–H region were unique for the two allomorphs, i.e. Iα and Iβ, while the spectral signatures in the C–H regions varied in all samples examined. Even though the origin of the different spectral signatures of the crystalline cellulose in the O–H and C–H vibrational frequency regions are yet to be correlated to the structure of cellulose, these results lead to new spectroscopic methods and opportunities to classify and to understand the basic crystalline structures, as well as variations in polymorphism of the crystalline cellulose.

  6. STAR FORMATION IN 30 DORADUS

    SciTech Connect

    De Marchi, Guido; Spezzi, Loredana; Sirianni, Marco; Andersen, Morten; Paresce, Francesco; Panagia, Nino; Mutchler, Max; Whitmore, Bradley C.; Bond, Howard; Beccari, Giacomo; Balick, Bruce; Dopita, Michael A.; Frogel, Jay A.; Calzetti, Daniela; Marcella Carollo, C.; Disney, Michael J.; Hall, Donald N. B.; Holtzman, Jon A.; Kimble, Randy A.; McCarthy, Patrick J.

    2011-09-20

    Using observations obtained with the Wide-Field Camera 3 on board the Hubble Space Telescope, we have studied the properties of the stellar populations in the central regions of 30 Dor in the Large Magellanic Cloud. The observations clearly reveal the presence of considerable differential extinction across the field. We characterize and quantify this effect using young massive main-sequence stars to derive a statistical reddening correction for most objects in the field. We then search for pre-main-sequence (PMS) stars by looking for objects with a strong (>4{sigma}) H{alpha} excess emission and find about 1150 of them over the entire field. Comparison of their location in the Hertzsprung-Russell diagram with theoretical PMS evolutionary tracks for the appropriate metallicity reveals that about one-third of these objects are younger than {approx}4 Myr, compatible with the age of the massive stars in the central ionizing cluster R 136, whereas the rest have ages up to {approx}30 Myr, with a median age of {approx}12 Myr. This indicates that star formation has proceeded over an extended period of time, although we cannot discriminate between an extended episode and a series of short and frequent bursts that are not resolved in time. While the younger PMS population preferentially occupies the central regions of the cluster, older PMS objects are more uniformly distributed across the field and are remarkably few at the very center of the cluster. We attribute this latter effect to photo-evaporation of the older circumstellar disks caused by the massive ionizing members of R 136.

  7. Prevention of diabetes in the BB rat by essential fatty acid deficiency. Relationship between physiological and biochemical changes

    PubMed Central

    1990-01-01

    Essential fatty acid (EFA) deficiency exerts a striking protective effect in several animal models of autoimmune disease. We now report that EFA deprivation prevents diabetes in the BB rat, an animal model of human insulin-dependent diabetes mellitus. In diabetes-prone (DP)-BB rats, the incidences of spontaneous diabetes and insulitis (the pathological substrate of autoimmune diabetes) were greatly reduced by EFA deficiency. This beneficial effect of the deficiency state was also seen in diabetes-resistant (DR)-BB rats that, after treatment with antibody to eliminate RT6+ T cells, would otherwise have become diabetic. The susceptibility of EFA-deprived DP-BB rats to spontaneous diabetes was restored when they were given dietary supplements of linoleate at 70 d of age (during the usual period of susceptibility), but not when they were repleted beginning at 120 d (after the peak incidence of diabetes). EFA deficiency did lead to growth retardation, but calorically restricted control rats demonstrated that the protective effect of the deficiency state was not a function of decreased weight. To examine the relationship between the biochemical changes of EFA deficiency and its physiological effects in this system, we compared the fatty acid changes that occurred in EFA-deficient animals that did and did not develop diabetes. Nondiabetic animals had significantly lower levels of (n-6) fatty acids (i.e., linoleate and arachidonate) and higher levels of oleate, an (n-9) fatty acid, than did diabetic animals. Levels of 20:3(n-9), the fatty acid that uniquely characterizes EFA deficiency, were similar in both groups, however. Among diabetic EFA-deficient rats, the age at onset of diabetes was found to correlate inversely with the level of (n-6) fatty acids, the least depleted animals becoming diabetic earliest, whereas there was no correlation with levels of 20:3(n-9). Among animals repleted with linoleate beginning at 70 d, restoration of susceptibility to diabetes

  8. Tumor cell and connective tissue cell interactions in human colorectal adenocarcinoma. Transfer of platelet-derived growth factor-AB/BB to stromal cells.

    PubMed Central

    Sundberg, C.; Branting, M.; Gerdin, B.; Rubin, K.

    1997-01-01

    Mechanisms underlying stimulation of platelet-derived growth factor (PDGF) beta-receptors expressed on connective tissue cells in human colorectal adenocarcinoma were investigated in this study. PDGF-AB/BB, but not PDGF receptors, was expressed by tumor cells in situ, as well as in tumor cell isolates of low passage from human colorectal adenocarcinoma. In an experimental co-culture system, conditioned medium from tumor cells only marginally activated PDGF beta-receptors expressed on fibroblasts. In contrast, co-culturing of the two cell types led to a marked PDGF beta-receptor activation. Functional PDGF-AB/BB was found to be associated with heparinase-I-sensitive components on the tumor cell surface. PDGF-AB/BB, isolated from heparinase-I-sensitive cell surface components, induced a marked activation of PDGF beta-receptors. Furthermore, co-culturing tumor cells together with fibroblasts led to a sustained activation of PDGF beta-receptors expressed on fibroblasts. Double immunofluorescence staining of tissue sections from human colorectal adenocarcinoma, combined with computer-aided image analysis, revealed that nonproliferating tumor cells were the predominant cellular source of PDGF-AB/BB in the tumor stroma. In addition, PDGF-AB/BB-expressing tumor cells were found juxtapositioned to microvascular cells expressing activated PDGF beta-receptors. Confocal microscopy revealed a cytoplasmic and cell-membrane-associated expression of PDGF-AB/BB in tumor cells situated in the stroma. In contrast, epithelial cells situated in normal or tumorous acinar structures revealed only a cell-membrane-associated PDGF-AB/BB expression. The is vitro and in situ results demonstrate that tumor cells not only facilitate but also have the ability to modulate connective tissue cell responsiveness to PDGF-AB/BB in a paracrine fashion, through direct cell-cell interactions in human colorectal adenocarcinoma. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:9250160

  9. Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration.

    PubMed

    Kim, Sung Eun; Yun, Young-Pil; Lee, Jae Yong; Shim, June-Sung; Park, Kyeongsoon; Huh, Jung-Bo

    2015-12-01

    The aim of this study was to improve osteoblast function by delivering two growth factors, PDGF-BB and BMP-2, incorporated onto heparinized titanium (Hep-Ti) substrate. To achieve co-delivery of PDGF-BB and BMP-2, the surface of anodized Ti was immobilized with heparin, and then the two growth factors were coated onto the Hep-Ti surface. Incorporation of the two growth factors onto Hep-Ti was evaluated by SEM and XPS. Incorporated PDGF-BB and BMP-2 were released from the Hep-Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. These results suggested that co-delivery of PDGF-BB and BMP-2 using Hep-Ti substrate will be a promising material for the enhancement of osteoblast function and osteointegration.

  10. Independent Action between DvSnf7 RNA and Cry3Bb1 Protein in Southern Corn Rootworm, Diabrotica undecimpunctata howardi and Colorado Potato Beetle, Leptinotarsa decemlineata

    PubMed Central

    Levine, Steven L.; Tan, Jianguo; Mueller, Geoffrey M.; Bachman, Pamela M.; Jensen, Peter D.; Uffman, Joshua P.

    2015-01-01

    In recent years, corn rootworm (CRW)-resistant maize events producing two or more CRW-active Bt proteins have been commercialized to enhance efficacy against the target pest(s) by providing multiple modes of action (MoA). The maize hybrid MON 87411 has been developed that produces the CRW-active Cry3Bb1 Bt protein (hereafter Cry3Bb1) and expresses a RNAi-mediated MoA that also targets CRW. As part of an environmental risk assessment for MON 87411, the potential for an interaction between the CRW-active DvSnf7 RNA (hereafter DvSnf7) and Cry3Bb1 was assessed in 12-day diet incorporation bioassays with the southern corn rootworm (SCR, Diabrotica undecimpunctata howardi). The potential for an interaction between DvSnf7 and Cry3Bb1 was evaluated with two established experimental approaches. The first approach evaluated each substance alone and in combination over three different response levels. For all three response levels, observed responses were shown to be additive and not significantly different from predicted responses under the assumption of independent action. The second approach evaluated the potential for a fixed sub-lethal concentration of Cry3Bb1 to decrease the median lethal concentration (LC50) of DvSnf7 and vice-versa. With this approach, the LC50 value of DvSnf7 was not altered by a sub-lethal concentration of Cry3Bb1 and vice-versa. In addition, the potential for an interaction between the Cry3Bb1 and DvSnf7 was tested with Colorado potato beetle (CPB, Leptinotarsa decemlineata), which is sensitive to Cry3Bb1 but not DvSnf7. CPB assays also demonstrated that DvSnf7 does not alter the activity of Cry3Bb1. The results from this study provide multiple lines of evidence that DvSnf7 and Cry3Bb1 produced in MON 87411 have independent action. PMID:25734482

  11. Effect of recombinant human platelet-derived growth factor-BB-coated sutures on Achilles tendon healing in a rat model: A histological and biomechanical study

    PubMed Central

    Cummings, Stephen H; Grande, Daniel A; Hee, Christopher K; Kestler, Hans K; Roden, Colleen M; Shah, Neil V; Razzano, Pasquale; Dines, David M; Chahine, Nadeen O

    2012-01-01

    Purpose: Repairing tendon injuries with recombinant human platelet-derived growth factor-BB has potential for improving surgical outcomes. Augmentation of sutures, a critical component of surgical tendon repair, by coating with growth factors may provide a clinically useful therapeutic device for improving tendon repair. Therefore, the purpose of this study was to (a) coat Vicryl sutures with a defined dose of recombinant human platelet-derived growth factor-BB without additional coating excipients (e.g. gelatin), (b) quantify the recombinant human platelet-derived growth factor-BB released from the suture, and (c) use the recombinant human platelet-derived growth factor-BB-coated sutures to enhance tendon repair in a rat Achilles tendon transection model. Methods: Vicryl sutures were coated with 0, 0.3, 1.0, and 10.0 mg/mL concentrations of recombinant human platelet-derived growth factor-BB using a dip-coating process. In vitro release was quantified by an enzyme-linked immunosorbent assay. Acutely transected rat Achilles tendons were repaired using one of the four suture groups (n = 12 per group). Four weeks following repair, the tensile biomechanical and histological (i.e. collagen organization and angiogenesis) properties were determined. Results: A dose-dependent bolus release of recombinant human platelet-derived growth factor-BB occurred within the first hour in vitro, followed by a gradual release over 48 h. There was a significant increase in ultimate tensile strength (p < 0.01) in the two highest recombinant human platelet-derived growth factor-BB dose groups (1.9 ± 0.5 and 2.1 ± 0.5 MPa) relative to controls (1.0 ± 0.2 MPa). The modulus significantly increased (p = 0.031) with the highest recombinant human platelet-derived growth factor-BB dose group (7.2 ± 3.8 MPa) relative to all other groups (control: 3.5 ± 0.9 MPa). No significant differences were identified for the maximum load or stiffness. The histological collagen and angiogenesis scores

  12. Independent action between DvSnf7 RNA and Cry3Bb1 protein in southern corn rootworm, Diabrotica undecimpunctata howardi and Colorado potato beetle, Leptinotarsa decemlineata.

    PubMed

    Levine, Steven L; Tan, Jianguo; Mueller, Geoffrey M; Bachman, Pamela M; Jensen, Peter D; Uffman, Joshua P

    2015-01-01

    In recent years, corn rootworm (CRW)-resistant maize events producing two or more CRW-active Bt proteins have been commercialized to enhance efficacy against the target pest(s) by providing multiple modes of action (MoA). The maize hybrid MON 87411 has been developed that produces the CRW-active Cry3Bb1 Bt protein (hereafter Cry3Bb1) and expresses a RNAi-mediated MoA that also targets CRW. As part of an environmental risk assessment for MON 87411, the potential for an interaction between the CRW-active DvSnf7 RNA (hereafter DvSnf7) and Cry3Bb1 was assessed in 12-day diet incorporation bioassays with the southern corn rootworm (SCR, Diabrotica undecimpunctata howardi). The potential for an interaction between DvSnf7 and Cry3Bb1 was evaluated with two established experimental approaches. The first approach evaluated each substance alone and in combination over three different response levels. For all three response levels, observed responses were shown to be additive and not significantly different from predicted responses under the assumption of independent action. The second approach evaluated the potential for a fixed sub-lethal concentration of Cry3Bb1 to decrease the median lethal concentration (LC50) of DvSnf7 and vice-versa. With this approach, the LC50 value of DvSnf7 was not altered by a sub-lethal concentration of Cry3Bb1 and vice-versa. In addition, the potential for an interaction between the Cry3Bb1 and DvSnf7 was tested with Colorado potato beetle (CPB, Leptinotarsa decemlineata), which is sensitive to Cry3Bb1 but not DvSnf7. CPB assays also demonstrated that DvSnf7 does not alter the activity of Cry3Bb1. The results from this study provide multiple lines of evidence that DvSnf7 and Cry3Bb1 produced in MON 87411 have independent action.

  13. Blocking the 4-1BB Pathway Ameliorates Crystalline Silica-induced Lung Inflammation and Fibrosis in Mice

    PubMed Central

    Li, Chao; Du, Sitong; Lu, Yiping; Lu, Xiaowei; Liu, Fangwei; Chen, Ying; Weng, Dong; Chen, Jie

    2016-01-01

    Long term pulmonary exposure to crystalline silica leads to silicosis that manifests progressive interstitial fibrosis, eventually leading to respiratory failure and death. Despite efforts to eliminate silicosis, clinical cases continue to occur in both developing and developed countries. The exact mechanisms of crystalline silica-induced pulmonary fibrosis remain elusive. Herein, we find that 4-1BB is induced in response to crystalline silica injury in lungs and that it is highly expressed during development of experimental silicosis. Therefore, we explore the role of 4-1BB pathway during crystalline silica-induced lung injury and find that a specific inhibitor blocking the pathway could effectively alleviate crystalline silica-induced lung inflammation and subsequent pulmonary fibrosis in vivo. Compared to controls, the treated mice exhibited reduced Th1 and Th17 responses. The concentrations of pro-inflammatory cytokines in bronchoalveolar lavage fluid (BALF), including tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-17A following crystalline silica challenge were also reduced in inhibitor-treated mice. Although there was no significant alteration in Th2 cytokines of IL-4 and IL-13, another type of pro-fibrogenic cell, regulatory T cell (Treg) was significantly affected. In addition, one of the major participants in fibrogenesis, fibrocyte recruited less due to the blockade. Furthermore, we demonstrated the decreased fibrocyte recruitment was associated with chemokine reductions in lung. Our study discovers the 4-1BB pathway signaling enhances inflammatory response and promotes pulmonary fibrosis induced by crystalline silica. The findings here provide novel insights into the molecular events that control crystalline silica-induced lung inflammation and fibrosis through regulating Th responses and the recruitment of fibrocytes in crystalline silica-exposed lung. PMID:27698940

  14. Expression of platelet-derived growth factor BB, erythropoietin and erythropoietin receptor in canine and feline osteosarcoma

    PubMed Central

    Meyer, F.R.L.; Steinborn, R.; Grausgruber, H.; Wolfesberger, B.; Walter, I.

    2015-01-01

    The discovery of expression of the erythropoietin receptor (EPO-R) on neoplastic cells has led to concerns about the safety of treating anaemic cancer patients with EPO. In addition to its endocrine function, the receptor may play a role in tumour progression through an autocrine mechanism. In this study, the expression of EPO, EPO-R and platelet-derived growth factor BB (PDGF-BB) was analysed in five feline and 13 canine osteosarcomas using immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR). EPO expression was positive in all tumours by IHC, but EPO mRNA was only detected in 38% of the canine and 40% of the feline samples. EPO-R was expressed in all samples by quantitative RT-PCR (RT-qPCR) and IHC. EPO-R mRNA was expressed at higher levels in all feline tumours, tumour cell lines, and kidney when compared to canine tissues. PDGF-BB expression was variable by IHC, but mRNA was detected in all samples. To assess the functionality of the EPO-R on tumour cells, the proliferation of canine and feline osteosarcoma cell lines was evaluated after EPO administration using an alamarBlue assay and Ki67 immunostaining. All primary cell lines responded to EPO treatment in at least one of the performed assays, but the effect on proliferation was very low indicating only a weak responsiveness of EPO-R. In conclusion, since EPO and its receptor are expressed by canine and feline osteosarcomas, an autocrine or paracrine tumour progression mechanism cannot be excluded, although in vitro data suggest a minimal role of EPO-R in osteosarcoma cell proliferation. PMID:26189892

  15. Characterisation of matrix metalloproteinases and the effects of a broad-spectrum inhibitor (BB-1101) in peripheral nerve regeneration.

    PubMed

    Demestre, M; Wells, G M; Miller, K M; Smith, K J; Hughes, R A C; Gearing, A J; Gregson, N A

    2004-01-01

    The effect of treatment with a broad-spectrum inhibitor (BB1101) of the matrix metalloproteinases (MMPs) on nerve regeneration and functional recovery after nerve crush was examined. Drug treatment had no effect on latency but from 63 days the compound muscle action potential was significantly increased and was no different to that in the sham-operated controls at 72 days. Levels of MMP mRNA expression, and the localisation and activity of MMP proteins, were examined in rats for a 2 month period following a nerve crush injury, and compared with sham-operated controls. The mRNA of all the MMPs studied was up-regulated by 5-10 days after nerve crush, and they remained up-regulated for 40-63 days, except for MMP-9 which was down-regulated by 10 days. MMP immunoreactivity was localised to Schwann cells, macrophages and endothelial cells, and with the exception of membrane type 1-MMP (MT1-MMP), it was more intense after nerve crush compared with sham-operated controls. Regenerating axons showed immunoreactivity for MMP-2 and MMP-3. In situ zymography confirmed that the activity of MMPs in the nerve was increased following crush but that the activity was greatly reduced in rats treated with BB-1101. Thus despite the inhibition of MMPs by BB-1101, the drug did not appear to essentially affect nerve degeneration or regeneration following nerve crush but that it could be beneficial in promoting the more effective reinnervation of muscles possibly by actions at the level of the muscles.

  16. Search for a Transit of Alpha Centauri Bb, the First Earth-mass Exoplanet Orbiting a Sun-like Star

    NASA Astrophysics Data System (ADS)

    Ehrenreich, David

    2012-10-01

    The eagerly awaited detection of an Earth-mass planet using the radial-velocity technique has been announced last week {Dumusque et al. 2012}. This planet, Alpha Cen Bb orbits our close neighbor alpha Cen B and has the potential to become a true Rosetta stone in exoplanet science, if its transiting nature were revealed. HST/STIS is the only facility able to detect a transit from such a small planet at a high confidence level. We propose to quasi-continuously monitor the expected transit window of Alpha Cen B b during 26 hours {16 orbits} to encompass the orbital solutions allowed by the published radial-velocity dataset, that we reanalyzed for this purpose. If alpha Cen Bb is transiting, our proposed observations will not only confirm the existence of the planet but also yield for the first time the density of an Earth-mass exoplanet and constrain its interior composition. Our observations using the G750M grating will provide the first spectrum of a terrestrial exoplanet, with possibly detectable water features. In the case of an exosphere tail, detection of the sodium doublet would be achievable, depending on the tail extension. Finally, the transit light curve shape will constrain alpha Cen Bb's orbital eccentricity. This would be an important constraint for planetary formation and evolution models in binary systems. If no transit is detected, our observations will still be important: detection of p-mode oscillations and granulation is expected for alpha Cen A and B, which will constrain convection and radiative transport modeling in the atmosphere of cool main-sequence stars.

  17. Immune Modulation through 4-1BB Enhances SIV Vaccine Protection in Non-Human Primates against SIVmac251 Challenge

    PubMed Central

    Morrow, Matthew P.; Jure-Kunkel, Maria N.; Weiner, David B.

    2011-01-01

    Costimulatory molecules play a central role in the development of cellular immunity. Understanding how costimulatory pathways can be directed to positively influence the immune response may be critical for the generation of an effective HIV vaccine. Here, we evaluated the ability of intravenous administration of a blocking monoclonal antibody (mAb) directed against the negative costimulatory molecule CTLA-4, and an agonist mAb directed against the positive costimulatory molecule 4-1BB, either alone or in combination, to augment intramuscular SIV DNA immunizations. We then tested the ability these of these responses to impact a high-dose SIVmac251 challenge. Following immunization, the groups infused with the anti-4-1BB mAb exhibited enhanced IFN-γ responses compared to the DNA vaccine only group. Interestingly, although CTLA-4 blockade alone did not enhance IFN-γ responses it did increase the proliferative capacity of the CD4+ and CD8+ T cells. The combination of both mAbs enhanced the magnitude of the polyfunctional CD8+ T cell response. Following challenge, the group that received both mAbs exhibited a significant, ∼2.0 log, decrease in plasma viral load compared to the naïve group the included complete suppression of viral load in some animals. Furthermore, the use of the CTLA-4 blocking antibody resulted in significantly higher viral loads during chronic infection compared to animals that received the 4-1BB mAb, likely due to the higher CD4+ T cell proliferative responses which were driven by this adjuvant following immunization. These novel studies show that these adjuvants induce differential modulation of immune responses, which have dramatically different consequences for control of SIV replication, suggesting important implications for HIV vaccine development. PMID:21935390

  18. Folic acid inhibits dedifferentiation of PDGF-BB-induced vascular smooth muscle cells by suppressing mTOR/P70S6K signaling

    PubMed Central

    Pan, Sunlei; Lin, Hui; Luo, Hangqi; Gao, Feidan; Meng, Liping; Zhou, Changzuan; Jiang, Chengjian; Guo, Yan; Ji, Zheng; Chi, Jufang; Guo, Hangyuan

    2017-01-01

    Objective: Folic acid (FA) supplementation reduces the risk of atherosclerosis and stroke. Phenotypic change from differentiated to dedifferentiated vascular smooth muscle cells (VSMCs) plays an important role in atherosclerosis development; however, the exact mechanisms remain unknown. This study aimed to assess whether FA through mammalian target of rapamycin (mTOR)/P70S6K signaling inhibits platelet derived growth factor (PDGF-BB) induced VSMC dedifferentiation. Methods: VSMCs from primary cultures were identified by morphological observation and α-smooth muscle actin (α-SM-actin, α-SMA) immunocytochemistry. Then, VSMCs were induced by PDGF-BB and treated with varying FA concentrations. Rapamycin and MHY-1485 were used to inhibit or activate the mTOR/P70S6K pathway, respectively. Next, MTT, Transwell, and wound healing assays were employed to assess proliferation and migration of VSMCs. In addition, Western blotting was used to evaluate protein levels of α-SMA, calponin, osteopontin, mTOR, p-mTOR, P70S6K and p-P70S6K in VSMCs. Results: VSMCs showed phenotypic alteration from differentiated to dedifferentiated cells in response to PDGF-BB. MTT, Transwell and wound healing assays showed that FA markedly inhibited proliferation and migration in PDGF-BB-induced VSMCs, in a time and concentration-dependent manner. FA treatment increased the expression levels of the contractile phenotype marker proteins α-SMA and calponin compared with VSMCs stimulated by PDGF-BB alone. Furthermore, FA significantly suppressed mTOR and P70S6K phosphorylation compared with PDGF-BB alone. Similar to FA, downregulation of mTOR signaling by rapamycin inhibited VSMC dedifferentiation. In contrast, upregulation of mTOR signaling by MHY-1485 reversed the FA-induced inhibition of VSMC dedifferentiation. Conclusion: Folic acid inhibits dedifferentiation of PDGF-BB-induced VSMCs by suppressing mTOR/P70S6K signaling. PMID:28386356

  19. Temporary, but Essential Requirement of CD8+ T Cells Early in the Pathogenesis of Diabetes in BB Rats as Revealed by Thymectomy and CD8 Depletion

    PubMed Central

    Groen, Herman; Klatter, Flip; Pater, Jennie; Nieuwenhuis, Paul; Rozing, Jan

    2003-01-01

    Autoimmunity-prone BB rats demonstrate a T lymphocytopenia and abnormal T cell subset distribution. To test whether the life span of all T cells or only of certain subsets is reduced in BB rats, we thymectomised 8-week-old BB and PVG rats and subsequently assessed size and composition of the T cell population over a 6-week-period. In both strains, thymectomy (Tx) was followed by a decrease in peripheral T cell numbers, which was proportionally larger in BB rats. The decline of the Thy-1+ recent thymic migrant (RTM) T cell phenotype was similar in both strains. BB rats showed a rapid preferential loss of CD8+ and CD45RC+ T cells, whereas the relative loss of RT6+ T cells was proportional to that of all T cells and not significantly different from that in PVG rats. Tx at 8-week did not prevent diabetes. Tx of 4-week-old BB rats revealed essentially the same changes in peripheral T cell subset distribution as in 8-week-old animals. However, Tx at week 4 did prevent diabetes. Since this raised the possibility of a temporary requirement of CD8+ T cells for the development of diabetes, we performed CD8 depletions during different pre-diabetic intervals. We found that CD8 depletion from 4 to 8 and 4 to 14 weeks, but not from 8 to 14 weeks of age prevented diabetes. We conclude that the protective effect of early adult Tx is, at least in part, due to the rapid loss of CD8+ T cells, and that these cells are only required between 4 and 8 weeks of age for diabetes to develop in BB rats. PMID:14768945

  20. Mating Success, Longevity, and Fertility of Diabrotica virgifera virgifera LeConte (Chrysomelidae: Coleoptera) in Relation to Body Size and Cry3Bb1-Resistant and Cry3Bb1-Susceptible Genotypes

    PubMed Central

    French, Bryan Wade; Hammack, Leslie; Tallamy, Douglas W.

    2015-01-01

    Insect resistance to population control methodologies is a widespread problem. The development of effective resistance management programs is often dependent on detailed knowledge regarding the biology of individual species and changes in that biology associated with resistance evolution. This study examined the reproductive behavior and biology of western corn rootworm beetles of known body size from lines resistant and susceptible to the Cry3Bb1 protein toxin expressed in transgenic Bacillus thuringiensis maize. In crosses between, and within, the resistant and susceptible genotypes, no differences occurred in mating frequency, copulation duration, courtship duration, or fertility; however, females mated with resistant males showed reduced longevity. Body size did not vary with genotype. Larger males and females were not more likely to mate than smaller males and females, but larger females laid more eggs. Moderately strong, positive correlation occurred between the body sizes of successfully mated males and females; however, weak correlation also existed for pairs that did not mate. Our study provided only limited evidence for fitness costs associated with the Cry3Bb1-resistant genotype that might reduce the persistence in populations of the resistant genotype but provided additional evidence for size-based, assortative mating, which could favor the persistence of resistant genotypes affecting body size. PMID:26569315

  1. An observational study of the eclipsing nova-like variable BH Lyncis (= PG 0818 + 513)

    NASA Astrophysics Data System (ADS)

    Dhillon, V. S.; Jones, D. H. P.; Marsh, T. R.; Smith, R. C.

    1992-09-01

    We present photometric and spectroscopic observations of the novalike variable BH Lyn. The continuum and line light curves exhibit deep eclipses which recur with a period of 3.74 hr. The spectra have single-peaked Balmer and He I emission lines which exhibit a double-peaked structure near the inferior conjunction of the emission-line source. Radial velocity measurements show large phase shifts in all the emission lines. We construct light-center and Doppler maps of the system which suggest that the line emission is largely nondisk in origin. We compare and contrast BH Lyn with a number of other eclipsing novalike variables. The objects show basically the same behavior, and we conclude that the emission lines must be produced by a similar (unknown) mechanism.

  2. THE RED NOVA-LIKE VARIABLE IN M31-A BLUE CANDIDATE IN QUIESCENCE

    SciTech Connect

    Shara, Michael M.; Zurek, David; Prialnik, Dina; Yaron, Ofer; Kovetz, Attay

    2010-12-10

    M31-RV was an extraordinarily luminous ({approx}10{sup 6} L{sub sun}) eruptive variable, displaying very cool temperatures (roughly 1000 K) as it faded. While this object's peak luminosity matched or exceeded those of the brightest known classical novae, its red colors and cool spectra were very different from those of classical novae. The photometric behavior of M31-RV (and several other very red novae, i.e., luminous eruptive red variables) has led to several models of this apparently new class of astrophysical object. We list these models, which predict very red eruptions and very red remnants decades after the eruptions. One of the most detailed models is that of 'mergebursts'. Mergebursts are (hypothetical) mergers of close binary stars, predicted to rival or exceed the brightest classical novae in luminosity, but to be much cooler and redder than classical novae, and to become slowly hotter and bluer as they age. This prediction suggests two stringent and definitive tests of the mergeburst hypothesis. First, there should always be a cool red remnant, and NOT a hot blue remnant at the site of such an outburst. Second, the inflated envelope of a mergeburst event should be slowly contracting; hence, it must display a slowly rising effective temperature. We have searched the location of M31-RV in multiple observatory archives. Our search revealed a luminous, UV-bright object within 0.''4 (1.5{sigma} of the astrometric position) of M31-RV in archival WFPC2 images taken 10 years after the outburst. Recent Hubble imagery, 20 years after the outburst, determines that this object is still hot and fading; it remains much too hot to be a mergeburst. Furthermore, the effective temperature of this object is declining, contrary to the prediction for mergebursts. If we have correctly identified M31-RV's remnant, it cannot be a mergeburst-but its behavior is consistent with theoretical nova models which erupt on a low-mass white dwarf. Future Hubble UV and visible images could determine if the M31-RV analogs (in M85 and in M99) are also much more blue than mergeburst theory predicts, and if they, too, are cooling in contradiction to mergeburst theory.

  3. DASCH DISCOVERY OF A POSSIBLE NOVA-LIKE OUTBURST IN A PECULIAR SYMBIOTIC BINARY

    SciTech Connect

    Tang Sumin; Grindlay, Jonathan E.; Moe, Maxwell; Kurucz, Robert L.; Quinn, Samuel N.; Servillat, Mathieu; Orosz, Jerome A.

    2012-06-01

    We present photometric and spectroscopic observations of a peculiar variable (designated DASCH J075731.1+201735 or J0757) discovered from our DASCH project using the digitized Harvard College Observatory archival photographic plates. It brightened by about 1.5 mag in B within a year starting in 1942, and then slowly faded back to its pre-outburst brightness from 1943 to 1950s. The mean brightness level was stable before and after the outburst, and ellipsoidal variations with a period of P = 119.18 {+-} 0.07 days are seen, suggesting that the star is tidally distorted. Radial-velocity measurements indicate that the orbit is nearly circular (e = 0.02 {+-} 0.01) with a spectroscopic period that is the same as the photometric period. The binary consists of a 1.1 {+-} 0.3 M{sub Sun} M0III star, and a 0.6 {+-} 0.2 M{sub Sun} companion, very likely a white dwarf (WD). Unlike other symbiotic binaries, there is no sign of emission lines or a stellar wind in the spectra. With an outburst timescale of {approx}10 years and estimated B-band peak luminosity M{sub B} {approx} 0.7, J0757 is different from any other known classic or symbiotic novae. The most probable explanation of the outburst is hydrogen shell burning on the WD, although an accretion-powered flare cannot be ruled out.

  4. Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system.

    PubMed

    Khan, Sehroon; Nadir, Sadia; Lihua, Guo; Xu, Jianchu; Holmes, Keith A; Dewen, Qiu

    2016-01-01

    An insect-toxic protein, Bb70p, was purified from Beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. Bb70p has a high affinity for anion exchangers and 2D electrophoresis results revealed a single spot with a molecular weight of 35.5 kDa and an iso-electric point of ∼4.5. Bb70p remains active from 4 to 60°C, within a pH range of 4-10, but is more active in slightly acidic pH. A pure protein, Bb70p does not have any carbohydrate side chains. The protein caused high mortality by intra-haemocelic injection into Galleria mellonella with LD50 of 334.4 μg/g body weight and activates the phenol oxidase cascade. With a partial amino acid sequence comparison using the NCBI database, we showed no homology to known toxin proteins of entomopathogenic fungi. Thus, Bb70p appears to be an insect toxin protein, demonstrating novelty. Identification of this insect-toxic protein presents potential to enhance the virulence of B. bassiana through genetic manipulation.

  5. Electroluminescent TCC, C3dg and fB/Bb epitope assays for profiling complement cascade activation in vitro using an activated complement serum calibration standard.

    PubMed

    van Vuuren, B Jansen; Bergseth, G; Mollnes, T E; Shaw, A M

    2014-01-15

    Electroluminescent assays for epitopes on the complement components C3dg, terminal complement complex (TCC) and factor B/Bb (fB/Bb) have been developed with capture and detection antibodies to produce detection limits C3dg=91±9ng/mL, TCC=3±0.1ng/mL and fB=55.7±0.1ng/mL. The assay performance was assessed against a series of zymosan and heat aggregated IgG (HAIgG) in vitro activations of complement using a calibrated activated complement serum (ACS) as calibration standard. The ACS standard was stable within 20% accuracy over a 6-month period with freeze-thaw cycles as required. Differential activation of the complement cascade was observed for TCC showing a pseudo-first order formation half-life of 3.5h after activation with zymosan. The C3dg activation fragment indicates a 10% total activation for both activation agents. The kinetic-epitope analysis for fB indicates that the capture epitope is on the fB/Bb protein fragment which can then become covered by the formation of C3bBb or C3bBbP complexes during the time course of the cascade.

  6. The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and -BB and inhibits the binding of PDGF to its receptors.

    PubMed Central

    Raines, E W; Lane, T F; Iruela-Arispe, M L; Ross, R; Sage, E H

    1992-01-01

    Interactions among growth factors, cells, and extracellular matrix are critical to the regulation of directed cell migration and proliferation associated with development, wound healing, and pathologic processes. Here we report the association of PDGF-AB and -BB, but not PDGF-AA, with the extracellular glycoprotein SPARC. Complexes of SPARC and 125I-labeled PDGF-BB or -AB were specifically immunoprecipitated by anti-SPARC immunoglobulins. 125I-PDGF-BB and -AB also bound specifically to SPARC that was immobilized on microtiter wells or bound to nitrocellulose after transfer from SDS/polyacrylamide gels. The binding of PDGF-BB to SPARC was pH-dependent; significant binding was detectable only above pH 6.6. The interaction of SPARC with specific dimeric forms of PDGF affected the activity of this mitogen. SPARC inhibited the binding of PDGF-BB and PDGF-AB, but not PDGF-AA, to human dermal fibroblasts in a dose-dependent manner. The expression of SPARC and PDGF was minimal in most normal adult tissues but was increased after injury. Enhanced expression of both PDGF-B chain and SPARC was seen in advanced lesions of atherosclerosis. We suggest that the coordinate expression of SPARC and PDGF-B-containing dimers following vascular injury may regulate the activity of specific dimeric forms of PDGF in vivo. Images PMID:1311092

  7. Apamin inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and migration through suppressions of activated Akt and Erk signaling pathway.

    PubMed

    Kim, Jung-Yeon; Kim, Kyung-Hyun; Lee, Woo-Ram; An, Hyun-Jin; Lee, Sun-Jae; Han, Sang-Mi; Lee, Kwang-Gill; Park, Yoon-Yub; Kim, Kee-Sik; Lee, Young-Soo; Park, Kwan-Kyu

    2015-07-01

    The increased proliferation and migration of vascular smooth muscle cells (VSMC) are key process in the development of atherosclerosis lesions. Platelet-derived growth factor (PDGF) initiates a multitude of biological effects that contribute to VSMC proliferation and migration. Apamin, a component of bee venom, has been known to block the Ca(2+)-activated K(+) channels. However, the effects of apamin in the regulation PDGF-BB-induced VSMC proliferation and migration has not been identified. In this study, we investigate the inhibitory effect of apamin on PDGF-BB-induced VSMC proliferation and migration. Apamin suppressed the PDGF-BB-induced VSMC proliferation and migration with no apparent cytotoxic effect. In accordance with these findings, apamin induced the arrest of cell cycle progression at G0/G1 phase. Apamin also decreased the expressions of G0/G1 specific regulatory proteins including proliferating cell nuclear antigen (PCNA), cyclin D1, cyclin-dependent kinases (CDK) 4, cyclin E and CDK2, as well as increased the expression of p21(Cip1) in PDGF-BB-induced VSMC. Moreover, apamin inhibited PDGF-BB-induced phosphorylation of Akt and Erk1/2. These results suggest that apamin plays an important role in prevention of vascular proliferation and migration through the G0/G1 cell cycle arrest by PDGF signaling pathway. Thus, apamin may be a promising candidate for the therapy of atherosclerosis.

  8. Cordyceps sinensis polysaccharide CPS-2 protects human mesangial cells from PDGF-BB-induced proliferation through the PDGF/ERK and TGF-β1/Smad pathways.

    PubMed

    Wang, Ying; Liu, Dan; Zhao, Huan; Jiang, Huixing; Luo, Chen; Wang, Min; Yin, Hongping

    2014-02-15

    CPS-2, a Cordyceps sinensis polysaccharide, has been demonstrated to have significant therapeutic activity against chronic renal failure. However, little is known about the underlying molecular mechanism. In this study, we found that CPS-2 could inhibit PDGF-BB-induced human mesangial cells (HMCs) proliferation in a dose-dependent manner. In addition, CPS-2 notably suppressed the expression of α-SMA, PDGF receptor-beta (PDGFRβ), TGF-β1, and Smad 3 in PDGF-BB-treated HMCs. Furthermore, PDGF-BB-stimulated ERK activation was significantly inhibited by CPS-2, and this inhibitory effect was synergistically potentiated by U0126. CPS-2 could prevent the PDGFRβ promoter activity induced by PDGF-BB, and return expression of PDGFRβ, TGF-β1, and TGFβRI to normal levels while cells were under PDGFRβ and ERK silencing conditions and transfected with DN-ERK. Taken together, these findings demonstrated that CPS-2 reduces PDGF-BB-induced cell proliferation through the PDGF/ERK and TGF-β1/Smad pathways, and it may have bi-directional regulatory effects on the PDGF/ERK cellular signaling pathway.

  9. Determination of soyasaponins Ba and Bb in human serum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

    PubMed

    Jin, Micong; Yang, Yiwen; Su, Baogen; Ren, Qilong

    2007-02-01

    A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS-MS) has been developed and validated for the determination of soyasaponins Ba and Bb in human serum using glycyrrhizin as internal standard (I.S.). Soyasaponins Ba and Bb were extracted from human serum by liquid-liquid extraction and cleaned up by C(18) solid-phase extraction (SPE), followed by separation on a C(18) reversed-phase column using acetonitrile/water containing 0.025% acetic acid as a mobile phase for gradient elution. Soyasaponins Ba and Bb, and I.S. were ionized by negative ion pneumatically assisted electrospray and detected by HPLC-MS-MS in the multiple-reaction monitoring (MRM) mode using precursor-->product ion combinations at m/z 958-->940, 942-->924 and 822-->351, respectively. The calibration curves were linear (r(2)>0.991) in the concentration range of 0.5-100.0 ng/mL, with lower limits of quantification of 0.5 and 0.3 ng/mL for soyasaponins Ba and Bb, respectively, in human serum. Intra-day and inter-day relative standard deviations (R.S.D.) were less than 7.9 and 11.3%, respectively. The mean recoveries of soyasaponins Ba and Bb ranged from 92 to 101% and from 85 to 94%, respectively.

  10. Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by suppression of PI3K/Akt and MAPK pathways

    SciTech Connect

    Chan, Chi-Ming; Fang, Jia-You; Lin, Hsin-Huang; Yang, Chi-Yea; Hung, Chi-Feng

    2009-10-09

    Retinal pigment epithelial (RPE) cells play a dominant role in the development of proliferative vitreoretinopathy (PVR), which is the leading cause of failure in retinal reattachment surgery. Several studies have shown that platelet-derived growth factor (PDGF) exhibits chemotaxis and proliferation effects on RPE cells in PVR. In this study, the inhibitory effect of lycopene on PDGF-BB-induced ARPE19 cell migration is examined. In electric cell-substrate impedance sensing (ECIS) and Transwell migration assays, significant suppression of PDGF-BB-induced ARPE19 cell migration by lycopene is observed. Cell viability assays show no cytotoxicity of lycopene on RPE cells. Lycopene shows no effect on ARPE19 cell adhesion and is found to inhibit PDGF-BB-induced tyrosine phosphorylation and the underlying signaling pathways of PI3K, Akt, ERK and p38 activation. However, PDGF-BB and lycopene show no effects on JNK activation. Taken together, our results demonstrate that lycopene inhibits PDGF-BB-induced ARPE19 cell migration through inhibition of PI3K/Akt, ERK and p38 activation.

  11. A nutritious medida (Sudanese cereal thin porridge) prepared by fermenting malted brown rice flour with Bifidobacterium longum BB 536.

    PubMed

    Kabier, Barka M; Mustafa, Suhaimi; Kharidah, Muhammad; Suraini, Abd-Aziz; Abdul Manap, Yazid

    2004-09-01

    The nutritive value of spontaneously fermented brown rice flour medida, a Sudanese cereal thin porridge, is low. This study was carried out to improve the nutritional quality of medida. The flour was soaked and malted at 30◦C to optimise the protein content. Flour malted for two days had the highest protein content. Skim milk was added to the malted brown rice flour medida and fermented using Bifidobacterium longum BB 536. Maximum count of B. longum BB 536 up to 9 log CFU/ ml was attained at 4.6 final fermentation pH. The resultant viscosity was similar to that of the spontaneously fermented brown rice flour medida. There was significant (P< 0.01) increase in both the energy density and the protein content, having increased 12 folds and 24 folds, respectively. The essential amino acids including lysine and methionine were highly augmented. The resultant medida have stable flowing characteristics and meet the whole protein and energy requirements for infants and children aged 1 - 10 years old.

  12. The Kallikrein Inhibitor from Bauhinia bauhinioides (BbKI) shows antithrombotic properties in venous and arterial thrombosis models.

    PubMed

    Brito, Marlon V; de Oliveira, Cleide; Salu, Bruno R; Andrade, Sonia A; Malloy, Paula M D; Sato, Ana C; Vicente, Cristina P; Sampaio, Misako U; Maffei, Francisco H A; Oliva, Maria Luiza V

    2014-05-01

    The Bauhinia bauhinioides Kallikrein Inhibitor (BbKI) is a Kunitz-type serine peptidase inhibitor of plant origin that has been shown to impair the viability of some tumor cells and to feature a potent inhibitory activity against human and rat plasma kallikrein (Kiapp 2.4 nmol/L and 5.2 nmol/L, respectively). This inhibitory activity is possibly responsible for an effect on hemostasis by prolonging activated partial thromboplastin time (aPTT). Because the association between cancer and thrombosis is well established, we evaluated the possible antithrombotic activity of this protein in venous and arterial thrombosis models. Vein thrombosis was studied in the vena cava ligature model in Wistar rats, and arterial thrombosis in the photochemical induced endothelium lesion model in the carotid artery of C57 black 6 mice. BbKI at a concentration of 2.0 mg/kg reduced the venous thrombus weight by 65% in treated rats in comparison to rats in the control group. The inhibitor prolonged the time for total artery occlusion in the carotid artery model mice indicating that this potent plasma kallikrein inhibitor prevented thrombosis.

  13. SARM modulates MyD88-mediated TLR activation through BB-loop dependent TIR-TIR interactions.

    PubMed

    Carlsson, Emil; Ding, Jeak Ling; Byrne, Bernadette

    2016-02-01

    Toll-like receptors (TLRs) recognise invading pathogens and initiate an innate immune response by recruiting intracellular adaptor proteins via heterotypic Toll/interleukin-1 receptor (TIR) domain interactions. Of the five TIR domain-containing adaptor proteins identified, Sterile α- and armadillo-motif-containing protein (SARM) is functionally unique; suppressing immune signalling instead of promoting it. Here we demonstrate that the recombinantly expressed and purified SARM TIR domain interacts with both the major human TLR adaptors, MyD88 and TRIF. A single glycine residue located in the BB-loop of the SARM TIR domain, G601, was identified as essential for interaction. A short peptide derived from this motif was also found to interact with MyD88 in vitro. SARM expression in HEK293 cells was found to significantly suppress lipopolysaccharide (LPS)-mediated upregulation of inflammatory cytokines, IL-8 and TNF-α, an effect lost in the G601A mutant. The same result was observed with cytokine activation initiated by MyD88 expression and stimulation of TLR2 with lipoteichoic acid (LTA), suggesting that SARM is capable of suppressing both TRIF- and MyD88- dependent TLR signalling. Our findings indicate that SARM acts on a broader set of target proteins than previously thought, and that the BB-loop motif is functionally important, giving further insight into the endogenous mechanisms used to suppress inflammation in immune cells.

  14. Irisin reverses platelet derived growth factor-BB-induced vascular smooth muscle cells phenotype modulation through STAT3 signaling pathway.

    PubMed

    Song, Haibo; Xu, Jia; Lv, Nan; Zhang, Yuzhu; Wu, Fei; Li, Huanjie; Shao, Lei; Mu, Qian; Wang, Fang; Tang, Dongqi; Fang, Xu

    2016-10-14

    Vascular smooth muscle cells (VSMCs) phenotype modulation toward a synthetic phenotype is the main cause of cardiovascular disease. As a newly discovered myokine, Irisin is thought to be a promising candidate for the treatment of metabolic disturbances, as well as cardiovascular disease. However, no evidence has been shown for the direct effect of Irisin on VSMCs phenotype modulation and its underling mechanisms. The aim of this study was to explore the effect of Irisin on VSMCs phenotype modulation and the mechanisms involved. In the present study, it was found that Irisin restored the PDGF-BB-induced VSMCs phenotype modulation which exhibited down-regulation of smooth muscle cells (SMC) expression and up-regulation of matrix synthesis related marker expression, as well as proliferative phenotype. Moreover, our research demonstrated that Irisin further activated STAT3 signaling pathways. Finally, by applying an STAT3 inhibitor, WP1066, we revealed the roles of STAT3 in the PDGF-BB-induced VSMCs phenotype modulation when they were treated with Irisin. Taken together, these results demonstrated that Irisin may play a crucial role in regulating VSMCs phenotype modulation via the STAT3 signaling pathway.

  15. Infection of peripancreatic lymph nodes but not islets precedes Kilham rat virus-induced diabetes in BB/Wor rats.

    PubMed Central

    Brown, D W; Welsh, R M; Like, A A

    1993-01-01

    A parvovirus serologically identified as Kilham rat virus (KRV) reproducibly induces acute type I diabetes in diabetes-resistant BB/Wor rats. The tissue tropism of KRV was investigated by in situ hybridization with a digoxigenin-labelled plasmid DNA probe containing approximately 1.6 kb of the genome of the UMass isolate of KRV. Partial sequencing of the KRV probe revealed high levels of homology to the sequence of minute virus of mice (89%) and to the sequence of H1 (99%), a parvovirus capable of infecting rats and humans. Of the 444 bases sequenced, 440 were shared by H1. KRV mRNA and DNA were readily detected in lymphoid tissues 5 days postinfection but were seldom seen in the pancreas. High levels of viral nucleic acids were observed in the thymus, spleen, and peripancreatic and cervical lymph nodes. The low levels of infection observed in the pancreas involved essentially only endothelial and interstitial cells. Beta cells of the pancreas were not infected with KRV. These findings suggest that widespread infection of peripancreatic and other lymphoid tissues but not pancreatic beta cells by KRV triggers autoimmune diabetes by perturbing the immune system of genetically predisposed BB/Wor rats. Images PMID:8371347

  16. Penetrating head injuries in children due to BB and pellet guns: a poorly recognized public health risk.

    PubMed

    Kumar, Ravi; Kumar, Ramesh; Mallory, Grant W; Jacob, Jeffrey T; Daniels, David J; Wetjen, Nicholas M; Foy, Andrew B; O'Neill, Brent R; Clarke, Michelle J

    2015-10-23

    OBJECT Nonpowder guns, defined as spring- or gas-powered BB or pellet guns, can be dangerous weapons that are often marketed to children. In recent decades, advances in compressed-gas technology have led to a significant increase in the power and muzzle velocity of these weapons. The risk of intracranial injury in children due to nonpowder weapons is poorly documented. METHODS A retrospective review was conducted at 3 institutions studying children 16 years or younger who had intracranial injuries secondary to nonpowder guns. RESULTS The authors reviewed 14 cases of intracranial injury in children from 3 institutions. Eleven (79%) of the 14 children were injured by BB guns, while 3 (21%) were injured by pellet guns. In 10 (71%) children, the injury was accidental. There was 1 recognized assault, but there were no suicide attempts; in the remaining 3 patients, the intention was indeterminate. There were no mortalities among the patients in this series. Ten (71%) of the children required operative intervention, and 6 (43%) were left with permanent neurological injuries, including epilepsy, cognitive deficits, hydrocephalus, diplopia, visual field cut, and blindness. CONCLUSIONS Nonpowder guns are weapons with the ability to penetrate a child's skull and brain. Awareness should be raised among parents, children, and policy makers as to the risk posed by these weapons.

  17. Impaired nitric oxide production in coronary endothelial cells of the spontaneously diabetic BB rat is due to tetrahydrobiopterin deficiency.

    PubMed Central

    Meininger, C J; Marinos, R S; Hatakeyama, K; Martinez-Zaguilan, R; Rojas, J D; Kelly, K A; Wu, G

    2000-01-01

    Endothelial cells (EC) from diabetic BioBreeding (BB) rats have an impaired ability to produce NO. This deficiency is not due to a defect in the constitutive isoform of NO synthase in EC (ecNOS) or alterations in intracellular calcium, calmodulin, NADPH or arginine levels. Instead, ecNOS cannot produce sufficient NO because of a deficiency in tetrahydrobiopterin (BH(4)), a cofactor necessary for enzyme activity. EC from diabetic rats exhibited only 12% of the BH(4) levels found in EC from normal animals or diabetes-prone animals which did not develop disease. As a result, NO synthesis by EC of diabetic rats was only 18% of that for normal animals. Increasing BH(4) levels with sepiapterin increased NO production, suggesting that BH(4) deficiency is a metabolic basis for impaired endothelial NO synthesis in diabetic BB rats. This deficiency is due to decreased activity of GTP-cyclohydrolase I, the first and rate-limiting enzyme in the de novo biosynthesis of BH(4). GTP-cyclohydrolase activity was low because of a decreased expression of the protein in the diabetic cells. PMID:10861247

  18. Forsythoside A Inhibits BVDV Replication via TRAF2-Dependent CD28–4-1BB Signaling in Bovine PBMCs

    PubMed Central

    Song, Quan-Jiang; Weng, Xiao-Gang; Cai, Dong-Jie; Zhang, Wang; Wang, Jiu-Feng

    2016-01-01

    Bovine viral diarrhea virus (BVDV), the causative agent of bovine viral diarrhea/mucosal disease (BVD/MD), is an important pathogen of cattle and other wild animals throughout the world. BVDV infection typically leads to an impaired immune response in cattle. In the present study, we investigated the effect of Forsythoside A (FTA) on BVDV infection of bovine peripheral blood mononuclear cells (PBMCs). We found that Forsythoside A could not only promote proliferation of PBMCs and T cells activation but also inhibit the replication of BVDV as well as apoptosis induced by BVDV. FTA treatment could counteract the BVDV-induced overproduction of IFN-γ to maintain the immune homeostasis in bovine PBMCs. At same time, FTA can enhance the secretion of IL-2. What’s more, BVDV promotes the expression of CD28, 4-1BB and TRAF-2, which can be modulated by FTA. Our data suggest that FTA protects PBMCs from BVDV infection possibly via TRAF2-dependent CD28–4-1BB signaling, which may activate PBMCs in response to BVDV infection. Therefore, this aids in the development of an effective adjuvant for vaccines against BVDV and other specific FTA-based therapies for preventing BVDV infection. PMID:27617959

  19. Stem Cells Cultured on Beta Tricalcium Phosphate (β-TCP) in Combination with Recombinant Human Platelet-Derived Growth Factor - BB (rh-PDGF-BB) for the Treatment of Human Infrabony Defects.

    PubMed

    Dhote, Roshani; Charde, Priti; Bhongade, Manohar; Rao, Jyotsana

    2015-01-01

    Knowledge gained from the field of tissue engineering, helped to develop a biological substitute that promotes tissue regeneration. The usual biological substitute consists of stem cells, growth factors and an appropriate scaffold. The present randomized controlled clinical and radiographic study was undertaken to evaluate the effectiveness of mesenchymal stem cells cultured on beta tricalcium phosphate (β-TCP) in combination with rh-PDGF-BB in treatment of infrabony defect in humans. A total of 24 infrabony defects in 14 systemically healthy patients were selected for the present study. The selected defects exhibited a probing pocket depth (PPD) of ≥ 5 mm and depth of infrabony component ≥ 3 mm as assessed by clinical and radiographic measurements and later confirmed by intrasurgical measurement. Baseline measurements included were Plaque Index (PI), Papillary Bleeding Index (PBI), Probing Pocket Depth (PPD), Relative gingival marginal level (RGML), Relative Clinical Attachment Level (R-CAL) and Radiographic Defect Depth (DD) and linear bone growth (LBG). 6 weeks after initial therapy, the defects were randomly assigned to either test group or control group. The control group was treated by an open flap debridement (OFD) only, while the test group was treated by a Stem cells cultured on β-TCP in combination with rh-PDGF-BB. All the measurements recorded preoperatively were repeated at 6 months after the surgery. The efficacy of each treatment modality was investigated through statistical analysis. Mean probing pocket depth reduction was significantly greater in test group (4.50 ± 1.08 mm) compared to the OFD group (3.50 ± 0.90 mm). Mean gains in clinical attachment level was 3.91 ± 1.37 mm in the test group and 2.08 ± 0.90 mm in the control group. The mean increase in gingival recession (GR) was less in test group (0.58 ± 0.79 mm) compared to OFD group (1.4 ± 0.66 mm). Radiographic defect depth reduction was greater in the test group (3.50 ± 0.67 mm

  20. Bacteriocin production by strain Lactobacillus delbrueckii ssp. bulgaricus BB18 during continuous prefermentation of yogurt starter culture and subsequent batch coagulation of milk.

    PubMed

    Simova, E D; Beshkova, D M; Angelov, M P; Dimitrov, Zh P

    2008-06-01

    By screening for bacteriocin-producing lactic acid bacteria of 1,428 strains isolated from authentic Bulgarian dairy products, Lb. bulgaricus BB18 strain obtained from kefir grain was selected. Out of 11 yogurt starters containing Lb. bulgaricus BB18 and S. thermophilus strains resistant to bacteriocin secreted by Lb. bulgaricus BB18 a yogurt culture (S. thermophilus 11A+Lb. bulgaricus BB18) with high growth and bacteriocinogenic activity in milk was selected. Continuous (pH-stat 5.7) prefermentation processes were carried out in milk at 37 degrees C in a 2l MBR bioreactor (MBR AG, Zurich, Switzerland) with an IMCS controller for agitation speed, temperature, dissolved oxygen, CO2 and pH. Prefermented milk with pH 5.7 coagulated in a thermostat at 37 degrees C until pH 4.8-4.9. S. thermophilus 11A and Lb. bulgaricus BB18 grew independently in a continuous mode at similar and sufficiently high-dilution rates (D=1.83 h(-1)-S. thermophilus 11A; D=1.80 h(-1)-Lb. bulgaricus BB18). The yogurt cultures developed in a stream at a high-dilution rate (D=2.03-2.28 h(-1)). The progress of both processes (growth and bacteriocin production) depended on the initial ratio between the two microorganisms. The continuous prefermentation process promoted conditions for efficient fermentation and bacteriocinogenesis of the starter culture during the batch process: strong reduction of the times for bacteriocin production and coagulation of milk (to 4.5-5.0 h); high cell productivity (lactobacilli-4x10(12) CFU ml(-1), streptococci-6x10(12) CFU ml(-1)); high productivity of bacteriocins (4,500 BU ml(-1))-1.7 times higher than the bacteriocinogenic activity of the batch starter culture.

  1. The role of NK cell activity in the pathogenesis of poly I:C accelerated and spontaneous diabetes in the diabetes prone BB rat.

    PubMed

    Sobel, D O; Azumi, N; Creswell, K; Holterman, D; Blair, O C; Bellanti, J A; Abbassi, V; Hiserodt, J C

    1995-12-01

    The development of insulin dependent diabetes mellitus (IDDM) and diabetes in the diabetes prone (DP) BB rat animal model of IDDM is thought to be due to an autoimmune process. Natural killer (NK) cells have been implicated but not proven to play a pathogenetic role in BB rats due to the increased NK cell number and activity found in these animals. We have recently reported that poly I:C, an inducer of cytokines and a potent enhancer of NK cell function, accelerates the development of diabetes in DP BB rats and induces diabetes in diabetes resistant (DR) BB rats. Since we have further demonstrated that poly I:C administration to BB rats increases NK cell number and levels of inducers of NK cell activity, interferon-alpha and IL-6 which is described therein, we tested the hypothesis that NK cell activity plays an important role in poly I:C accelerated disease. The role of NK cells in poly I:C accelerated diabetes and spontaneous diabetes was examined by determining whether selective depletion of NK cells using a rat NK cell specific antibody (anti-NKR-P1 antibody) alters the development of diabetes. The treatment of BB rats with anti-NKR-P1 antibody resulted in a significantly lower mean NK cell activity of splenic mononuclear cells than that found in control animals. However, the development of diabetes and degree of insulitis was not significantly different between treatment groups. BB rats administered anti-NKR-P1 antibody with poly I:C had a lower mean splenocyte NK cell activity and lower mean NK cell number within the peripheral blood and inflamed islets than rats administered poly I:C alone. However, anti-NKR-P1 antibody administration did not alter the accelerated development of diabetes or the degree of insulitis in poly I:C treated animals. These data document that NK cells do not play a major role in the pathogenesis of poly I:C accelerated diabetes or spontaneous diabetes in the DP BB rat.

  2. Camptothecin inhibits platelet-derived growth factor-BB-induced proliferation of rat aortic vascular smooth muscle cells through inhibition of PI3K/Akt signaling pathway

    SciTech Connect

    Park, Eun-Seok; Kang, Shin-il; Yoo, Kyu-dong; Lee, Mi-Yea; Yoo, Hwan-Soo; Hong, Jin-Tae; Shin, Hwa-Sup; Kim, Bokyung; Yun, Yeo-Pyo

    2013-04-15

    The abnormal proliferation of vascular smooth muscle cells (VSMCs) in arterial wall is a major cause of vascular disorders such as atherosclerosis and restenosis after angioplasty. In this study, we investigated not only the inhibitory effects of camptothecin (CPT) on PDGF-BB-induced VSMC proliferation, but also its molecular mechanism of this inhibition. CPT significantly inhibited proliferation with IC50 value of 0.58 μM and the DNA synthesis of PDGF-BB-stimulated VSMCs in a dose-dependent manner (0.5–2 μM ) without any cytotoxicity. CPT induced the cell cycle arrest at G0/G1 phase. Also, CPT decreased the expressions of G0/G1-specific regulatory proteins including cyclin-dependent kinase (CDK)2, cyclin D1 and PCNA in PDGF-BB-stimulated VSMCs. Pre-incubation of VSMCs with CPT significantly inhibited PDGF-BB-induced Akt activation, whereas CPT did not affect PDGF-receptor beta phosphorylation, extracellular signal-regulated kinase (ERK) 1/2 phosphorylation and phospholipase C (PLC)-γ1 phosphorylation in PDGF-BB signaling pathway. Our data showed that CPT pre-treatment inhibited VSMC proliferation, and that the inhibitory effect of CPT was enhanced by LY294002, a PI3K inhibitor, on PDGF-BB-induced VSMC proliferation. In addition, inhibiting the PI3K/Akt pathway by LY294002 significantly enhanced the suppression of PCNA expression and Akt activation by CPT. These results suggest that the anti-proliferative activity of CPT is mediated in part by downregulating the PI3K/Akt signaling pathway. - Highlights: ► CPT inhibits proliferation of PDGF-BB-induced VSMC without cytotoxicity. ► CPT arrests the cell cycle in G0/G1 phase by downregulation of cyclin D1 and CDK2. ► CPT significantly attenuates Akt phosphorylation in PDGF-BB signaling pathway. ► LY294002 enhanced the inhibitory effect of CPT on VSMC proliferation. ► Thus, CPT is mediated by downregulating the PI3K/Akt signaling pathway.

  3. Control of human endometrial stromal cell motility by PDGF-BB, HB-EGF and trophoblast-secreted factors.

    PubMed

    Schwenke, Maren; Knöfler, Martin; Velicky, Philipp; Weimar, Charlotte H E; Kruse, Michelle; Samalecos, Annemarie; Wolf, Anja; Macklon, Nick S; Bamberger, Ana-Maria; Gellersen, Birgit

    2013-01-01

    Human implantation involves extensive tissue remodeling at the fetal-maternal interface. It is becoming increasingly evident that not only trophoblast, but also decidualizing endometrial stromal cells are inherently motile and invasive, and likely contribute to the highly dynamic processes at the implantation site. The present study was undertaken to further characterize the mechanisms involved in the regulation of endometrial stromal cell motility and to identify trophoblast-derived factors that modulate migration. Among local growth factors known to be present at the time of implantation, heparin-binding epidermal growth factor-like growth factor (HB-EGF) triggered chemotaxis (directed locomotion), whereas platelet-derived growth factor (PDGF)-BB elicited both chemotaxis and chemokinesis (non-directed locomotion) of endometrial stromal cells. Supernatants of the trophoblast cell line AC-1M88 and of first trimester villous explant cultures stimulated chemotaxis but not chemokinesis. Proteome profiling for cytokines and angiogenesis factors revealed neither PDGF-BB nor HB-EGF in conditioned media from trophoblast cells or villous explants, while placental growth factor, vascular endothelial growth factor and PDGF-AA were identified as prominent secretory products. Among these, only PDGF-AA triggered endometrial stromal cell chemotaxis. Neutralization of PDGF-AA in trophoblast conditioned media, however, did not diminish chemoattractant activity, suggesting the presence of additional trophoblast-derived chemotactic factors. Pathway inhibitor studies revealed ERK1/2, PI3 kinase/Akt and p38 signaling as relevant for chemotactic motility, whereas chemokinesis depended primarily on PI3 kinase/Akt activation. Both chemotaxis and chemokinesis were stimulated upon inhibition of Rho-associated, coiled-coil containing protein kinase. The chemotactic response to trophoblast secretions was not blunted by inhibition of isolated signaling cascades, indicating activation of

  4. Human monocyte spreading induced by factor Bb of the alternative pathway of complement activation. A possible role for C5 in monocyte spreading

    PubMed Central

    1981-01-01

    The central serine esterase of the alternative pathway of complement (APC) activation, activated factor B (Bb), has been shown recently to induce murine macrophages and human monocytes to become spread on a glass substrata. It has also been established that to induce the spreading reaction, the catalytic site of the Bb enzyme must be structurally intact since treatment of Bb with heat (56 degrees C for 30 min) or diisopropylfluorophosphate (10(-3) M) destroyed both enzymatic and spreading activities. In the C3b,Bb complex, Bb exhibits restricted substrate specificity for C3 and C5. With this in mind, the role of C3 and C5 in the monocyte spreading reaction was explored in the present study. Expression of C3 and C5 on the surface of human peripheral blood monocytes was investigated by the direct fluorescent antibody technique employing fluorescein isothiocyanate-conjugated anti- C3 or C5 F(ab')2 antibody fragments. It was found that C3 and C5 were present on 6 +/- 7% of freshly prepared monocytes and that expression of C5, but not C3, increased to 70 +/- 6% when monocytes were incubated for 3 d in serum-free medium. Biosynthesis of C5 was indicated when it was found that under serum-free conditions, monocytes incorporated [3H]leucine into immunoprecipitable C5 with an apparent mol wt of 180,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The role of C3 and C5 in the monocyte spreading reaction induced by factor Bb was explored by testing for the ability of anti-C3 and anti- C5 Fab' antibody fragments to block monocyte spreading. It was found that anti-C5 Fab' inhibited by up to 100% the 3-h human monocyte spreading reaction induced by Bb; in contrast, anti-C3 Fab' or anti-C4 Fab' inhibited by less than 10%. That the inhibitory effect of anti-C5 Fab' was exerted directly on the monocyte was established when it was found that the 3-h monocyte spreading reaction was significantly inhibited by pretreating monocytes with anti-C5 Fab' for 20 min and then

  5. Anomalous D-Log E curve with high contrast developer Kodak D8 on ultra fine grain emulsion BB640.

    PubMed

    Ulibarrena, M; Mendez, M; Blaya, S; Fimia, A

    2001-12-03

    D-Log E curves, also known as H-D curves, are used since the XIX century as a tool for describing the characteristics of silver halide emulsions. This curve has a very standard shape, with a linear region, a toe, a shoulder and a solarization region. In this work we present a distortion of the usual curve due to the action of a high contrast developer, Kodak D8, on an ultra fine grain emulsion, BB640\\cite{ov04}. The solarization effect is replaced by a linear zone where developed densities increase with increasing exposures, until all silver halide present in the emulsion is reduced by developer D8 to metallic silver. Densities higher than 11 have been obtained.

  6. Two-loop radiative corrections of electroweak mixing angle and branching fraction for Z going to bb

    NASA Astrophysics Data System (ADS)

    Huang, Yi-Cheng

    In this thesis I develop a numerical technique which is based on the Mellin-Barnes representation to calculate two-loop Feynman integrals. The resulting complex integrals of high dimensions are being applied with some treatments, such as the variable transform, reduction formulas, etc, to improve the convergence of the integrals. The approach is adopted to compute the two-loop radiative corrections of the electroweak mixing angle, sin theta W, and the hadronic branching ratio Rb for the process Z → bb¯. I focus on contributions with an internal fermion sub-loop using the on-shell renormalization scheme. The results will help to derive improved constraints on the Higgs particle.

  7. Combined search for the standard model Higgs boson decaying to bb using the D0 run II data set.

    PubMed

    Abazov, V M; Abbott, B; Acharya, B S; Adams, M; Adams, T; Alexeev, G D; Alkhazov, G; Alton, A; Alverson, G; Askew, A; Atkins, S; Augsten, K; Avila, C; Badaud, F; Bagby, L; Baldin, B; Bandurin, D V; Banerjee, S; Barberis, E; Baringer, P; Bartlett, J F; Bassler, U; Bazterra, V; Bean, A; Begalli, M; Bellantoni, L; Beri, S B; Bernardi, G; Bernhard, R; Bertram, I; Besançon, M; Beuselinck, R; Bhat, P C; Bhatia, S; Bhatnagar, V; Blazey, G; Blessing, S; Bloom, K; Boehnlein, A; Boline, D; Boos, E E; Borissov, G; Bose, T; Brandt, A; Brandt, O; Brock, R; Bross, A; Brown, D; Brown, J; Bu, X B; Buehler, M; Buescher, V; Bunichev, V; Burdin, S; Buszello, C P; Camacho-Pérez, E; Casey, B C K; Castilla-Valdez, H; Caughron, S; Chakrabarti, S; Chakraborty, D; Chan, K M; Chandra, A; Chapon, E; Chen, G; Chevalier-Théry, S; Cho, D K; Cho, S W; Choi, S; Choudhary, B; Cihangir, S; Claes, D; Clutter, J; Cooke, M; Cooper, W E; Corcoran, M; Couderc, F; Cousinou, M-C; Croc, A; Cutts, D; Das, A; Davies, G; de Jong, S J; De La Cruz-Burelo, E; Déliot, F; Demina, R; Denisov, D; Denisov, S P; Desai, S; Deterre, C; Devaughan, K; Diehl, H T; Diesburg, M; Ding, P F; Dominguez, A; Dubey, A; Dudko, L V; Duggan, D; Duperrin, A; Dutt, S; Dyshkant, A; Eads, M; Edmunds, D; Ellison, J; Elvira, V D; Enari, Y; Evans, H; Evdokimov, A; Evdokimov, V N; Facini, G; Feng, L; Ferbel, T; Fiedler, F; Filthaut, F; Fisher, W; Fisk, H E; Fortner, M; Fox, H; Fuess, S; Garcia-Bellido, A; García-González, J A; García-Guerra, G A; Gavrilov, V; Gay, P; Geng, W; Gerbaudo, D; Gerber, C E; Gershtein, Y; Ginther, G; Golovanov, G; Goussiou, A; Grannis, P D; Greder, S; Greenlee, H; Grenier, G; Gris, Ph; Grivaz, J-F; Grohsjean, A; Grünendahl, S; Grünewald, M W; Guillemin, T; Gutierrez, G; Gutierrez, P; Hagopian, S; Haley, J; Han, L; Harder, K; Harel, A; Hauptman, J M; Hays, J; Head, T; Hebbeker, T; Hedin, D; Hegab, H; Heinson, A P; Heintz, U; Hensel, C; Heredia De La Cruz, I; Herner, K; Hesketh, G; Hildreth, M D; Hirosky, R; Hoang, T; Hobbs, J D; Hoeneisen, B; Hogan, J; Hohlfeld, M; Howley, I; Hubacek, Z; Hynek, V; Iashvili, I; Ilchenko, Y; Illingworth, R; Ito, A S; Jabeen, S; Jaffré, M; Jayasinghe, A; Jeong, M S; Jesik, R; Jiang, P; Johns, K; Johnson, E; Johnson, M; Jonckheere, A; Jonsson, P; Joshi, J; Jung, A W; Juste, A; Kaadze, K; Kajfasz, E; Karmanov, D; Kasper, P A; Katsanos, I; Kehoe, R; Kermiche, S; Khalatyan, N; Khanov, A; Kharchilava, A; Kharzheev, Y N; Kiselevich, I; Kohli, J M; Kozelov, A V; Kraus, J; Kulikov, S; Kumar, A; Kupco, A; Kurča, T; Kuzmin, V A; Lammers, S; Landsberg, G; Lebrun, P; Lee, H S; Lee, S W; Lee, W M; Lei, X; Lellouch, J; Li, D; Li, H; Li, L; Li, Q Z; Lim, J K; Lincoln, D; Linnemann, J; Lipaev, V V; Lipton, R; Liu, H; Liu, Y; Lobodenko, A; Lokajicek, M; Lopes de Sa, R; Lubatti, H J; Luna-Garcia, R; Lyon, A L; Maciel, A K A; Madar, R; Magaña-Villalba, R; Malik, S; Malyshev, V L; Maravin, Y; Martínez-Ortega, J; McCarthy, R; McGivern, C L; Meijer, M M; Melnitchouk, A; Menezes, D; Mercadante, P G; Merkin, M; Meyer, A; Meyer, J; Miconi, F; Mondal, N K; Mulhearn, M; Nagy, E; Naimuddin, M; Narain, M; Nayyar, R; Neal, H A; Negret, J P; Neustroev, P; Nguyen, H T; Nunnemann, T; Orduna, J; Osman, N; Osta, J; Padilla, M; Pal, A; Parashar, N; Parihar, V; Park, S K; Partridge, R; Parua, N; Patwa, A; Penning, B; Perfilov, M; Peters, Y; Petridis, K; Petrillo, G; Pétroff, P; Pleier, M-A; Podesta-Lerma, P L M; Podstavkov, V M; Popov, A V; Prewitt, M; Price, D; Prokopenko, N; Qian, J; Quadt, A; Quinn, B; Rangel, M S; Ranjan, K; Ratoff, P N; Razumov, I; Renkel, P; Ripp-Baudot, I; Rizatdinova, F; Rominsky, M; Ross, A; Royon, C; Rubinov, P; Ruchti, R; Sajot, G; Salcido, P; Sánchez-Hernández, A; Sanders, M P; Santos, A S; Savage, G; Sawyer, L; Scanlon, T; Schamberger, R D; Scheglov, Y; Schellman, H; Schlobohm, S; Schwanenberger, C; Schwienhorst, R; Sekaric, J; Severini, H; Shabalina, E; Shary, V; Shaw, S; Shchukin, A A; Shivpuri, R K; Simak, V; Skubic, P; Slattery, P; Smirnov, D; Smith, K J; Snow, G R; Snow, J; Snyder, S; Söldner-Rembold, S; Sonnenschein, L; Soustruznik, K; Stark, J; Stoyanova, D A; Strauss, M; Suter, L; Svoisky, P; Takahashi, M; Titov, M; Tokmenin, V V; Tsai, Y-T; Tschann-Grimm, K; Tsybychev, D; Tuchming, B; Tully, C; Uvarov, L; Uvarov, S; Uzunyan, S; Van Kooten, R; van Leeuwen, W M; Varelas, N; Varnes, E W; Vasilyev, I A; Verdier, P; Verkheev, A Y; Vertogradov, L S; Verzocchi, M; Vesterinen, M; Vilanova, D; Vokac, P; Wahl, H D; Wang, M H L S; Wang, R-J; Warchol, J; Watts, G; Wayne, M; Weichert, J; Welty-Rieger, L; White, A; Wicke, D; Williams, M R J; Wilson, G W; Wobisch, M; Wood, D R; Wyatt, T R; Xie, Y; Yamada, R; Yang, S; Yang, W-C; Yasuda, T; Yatsunenko, Y A; Ye, W; Ye, Z; Yin, H; Yip, K; Youn, S W; Yu, J M; Zennamo, J; Zhao, T; Zhao, T G; Zhou, B; Zhu, J; Zielinski, M; Zieminska, D; Zivkovic, L

    2012-09-21

    We present the results of the combination of searches for the standard model Higgs boson produced in association with a W or Z boson and decaying into bb using the data sample collected with the D0 detector in pp collisions at √s = 1.96 TeV at the Fermilab Tevatron Collider. We derive 95% C.L. upper limits on the Higgs boson cross section relative to the standard model prediction in the mass range 100 GeV ≤ M(H) ≤ 150 GeV, and we exclude Higgs bosons with masses smaller than 102 GeV at the 95% C.L. In the mass range 120 GeV ≤ M(H) ≤145 GeV, the data exhibit an excess above the background prediction with a global significance of 1.5 standard deviations, consistent with the expectation in the presence of a standard model Higgs boson.

  8. Combined search for the standard model Higgs boson decaying to a bb pair using the full CDF data set.

    PubMed

    Aaltonen, T; Álvarez González, B; Amerio, S; Amidei, D; Anastassov, A; Annovi, A; Antos, J; Apollinari, G; Appel, J A; Arisawa, T; Artikov, A; Asaadi, J; Ashmanskas, W; Auerbach, B; Aurisano, A; Azfar, F; Badgett, W; Bae, T; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Barria, P; Bartos, P; Bauce, M; Bedeschi, F; Behari, S; Bellettini, G; Bellinger, J; Benjamin, D; Beretvas, A; Bhatti, A; Binkley, M E; Bisello, D; Bizjak, I; Bland, K R; Blumenfeld, B; Bocci, A; Bodek, A; Bortoletto, D; Boudreau, J; Boveia, A; Brigliadori, L; Bromberg, C; Brucken, E; Budagov, J; Budd, H S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Calamba, A; Calancha, C; Camarda, S; Campanelli, M; Campbell, M; Canelli, F; Carls, B; Carlsmith, D; Carosi, R; Carrillo, S; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavaliere, V; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, K; Chokheli, D; Chung, W H; Chung, Y S; Ciocci, M A; Clark, A; Clarke, C; Compostella, G; Convery, M E; Conway, J; Corbo, M; Cordelli, M; Cox, C A; Cox, D J; Crescioli, F; Cuevas, J; Culbertson, R; Dagenhart, D; d'Ascenzo, N; Datta, M; de Barbaro, P; Dell'Orso, M; Demortier, L; Deninno, M; Devoto, F; d'Errico, M; Di Canto, A; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Donati, S; Dong, P; Dorigo, M; Dorigo, T; Ebina, K; Elagin, A; Eppig, A; Erbacher, R; Errede, S; Ershaidat, N; Eusebi, R; Farrington, S; Feindt, M; Fernandez, J P; Ferrazza, C; Field, R; Flanagan, G; Forrest, R; Frank, M J; Franklin, M; Freeman, J C; Funakoshi, Y; Furic, I; Gallinaro, M; Garcia, J E; Garfinkel, A F; Garosi, P; Gerberich, H; Gerchtein, E; Giagu, S; Giakoumopoulou, V; Giannetti, P; Gibson, K; Ginsburg, C M; Giokaris, N; Giromini, P; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldin, D; Goldschmidt, N; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Grinstein, S; Grosso-Pilcher, C; Group, R C; Guimaraes da Costa, J; Hahn, S R; Halkiadakis, E; Hamaguchi, A; Han, J Y; Happacher, F; Hara, K; Hare, D; Hare, M; Harr, R F; Hatakeyama, K; Hays, C; Heck, M; Heinrich, J; Herndon, M; Hewamanage, S; Hocker, A; Hopkins, W; Horn, D; Hou, S; Hughes, R E; Hurwitz, M; Husemann, U; Hussain, N; Hussein, M; Huston, J; Introzzi, G; Iori, M; Ivanov, A; James, E; Jang, D; Jayatilaka, B; Jeans, D T; Jeon, E J; Jindariani, S; Jones, M; Joo, K K; Jun, S Y; Junk, T R; Kamon, T; Karchin, P E; Kasmi, A; Kato, Y; Ketchum, W; Keung, J; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kim, Y J; Kimura, N; Kirby, M; Klimenko, S; Knoepfel, K; Kondo, K; Kong, D J; Konigsberg, J; Kotwal, A V; Kreps, M; Kroll, J; Krop, D; Kruse, M; Krutelyov, V; Kuhr, T; Kurata, M; Kwang, S; Laasanen, A T; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; LeCompte, T; Lee, E; Lee, H S; Lee, J S; Lee, S W; Leo, S; Leone, S; Lewis, J D; Limosani, A; Lin, C-J; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, C; Liu, H; Liu, Q; Liu, T; Lockwitz, S; Loginov, A; Lucchesi, D; Lueck, J; Lujan, P; Lukens, P; Lungu, G; Lys, J; Lysak, R; Madrak, R; Maeshima, K; Maestro, P; Malik, S; Manca, G; Manousakis-Katsikakis, A; Margaroli, F; Marino, C; Martínez, M; Mastrandrea, P; Matera, K; Mattson, M E; Mazzacane, A; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Mesropian, C; Miao, T; Mietlicki, D; Mitra, A; Miyake, H; Moed, S; Moggi, N; Mondragon, M N; Moon, C S; Moore, R; Morello, M J; Morlock, J; Movilla Fernandez, P; Mukherjee, A; Muller, Th; Murat, P; Mussini, M; Nachtman, J; Nagai, Y; Naganoma, J; Nakano, I; Napier, A; Nett, J; Neu, C; Neubauer, M S; Nielsen, J; Nodulman, L; Noh, S Y; Norniella, O; Oakes, L; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Orava, R; Ortolan, L; Pagan Griso, S; Pagliarone, C; Palencia, E; Papadimitriou, V; Paramonov, A A; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Pianori, E; Pilot, J; Pitts, K; Plager, C; Pondrom, L; Poprocki, S; Potamianos, K; Prokoshin, F; Pranko, A; Ptohos, F; Punzi, G; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Renton, P; Rescigno, M; Riddick, T; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rodriguez, T; Rogers, E; Rolli, S; Roser, R; Ruffini, F; Ruiz, A; Russ, J; Rusu, V; Safonov, A; Sakumoto, W K; Sakurai, Y; Santi, L; Sato, K; Saveliev, V; Savoy-Navarro, A; Schlabach, P; Schmidt, A; Schmidt, E E; Schwarz, T; Scodellaro, L; Scribano, A; Scuri, F; Seidel, S; Seiya, Y; Semenov, A; Sforza, F; Shalhout, S Z; Shears, T; Shepard, P F; Shimojima, M; Shochet, M; Shreyber-Tecker, I; Simonenko, A; Sinervo, P; Sliwa, K; Smith, J R; Snider, F D; Soha, A; Sorin, V; Song, H; Squillacioti, P; Stancari, M; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Strycker, G L; Sudo, Y; Sukhanov, A; Suslov, I; Takemasa, K; Takeuchi, Y; Tang, J; Tecchio, M; Teng, P K; Thom, J; Thome, J; Thompson, G A; Thomson, E; Tipton, P; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Totaro, P; Trovato, M; Ukegawa, F; Uozumi, S; Varganov, A; Vázquez, F; Velev, G; Vellidis, C; Vidal, M; Vila, I; Vilar, R; Vizán, J; Vogel, M; Volpi, G; Wagner, P; Wagner, R L; Wakisaka, T; Wallny, R; Wang, S M; Warburton, A; Waters, D; Wester, W C; Whiteson, D; Wicklund, A B; Wicklund, E; Wilbur, S; Wick, F; Williams, H H; Wilson, J S; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, H; Wright, T; Wu, X; Wu, Z; Yamamoto, K; Yamato, D; Yang, T; Yang, U K; Yang, Y C; Yao, W-M; Yeh, G P; Yi, K; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanetti, A; Zeng, Y; Zhou, C; Zucchelli, S

    2012-09-14

    We combine the results of searches for the standard model (SM) Higgs boson based on the full CDF Run II data set obtained from sqrt[s]=1.96  TeV pp collisions at the Fermilab Tevatron corresponding to an integrated luminosity of 9.45  fb(-1). The searches are conducted for Higgs bosons that are produced in association with a W or Z boson, have masses in the range 90-150  GeV/c(2), and decay into bb pairs. An excess of data is present that is inconsistent with the background prediction at the level of 2.5 standard deviations (the most significant local excess is 2.7 standard deviations).

  9. Existence and stability, and discrete BB and rank conditions, for general mixed-hybrid finite elements in elasticity

    NASA Technical Reports Server (NTRS)

    Xue, W.-M.; Atluri, S. N.

    1985-01-01

    In this paper, all possible forms of mixed-hybrid finite element methods that are based on multi-field variational principles are examined as to the conditions for existence, stability, and uniqueness of their solutions. The reasons as to why certain 'simplified hybrid-mixed methods' in general, and the so-called 'simplified hybrid-displacement method' in particular (based on the so-called simplified variational principles), become unstable, are discussed. A comprehensive discussion of the 'discrete' BB-conditions, and the rank conditions, of the matrices arising in mixed-hybrid methods, is given. Some recent studies aimed at the assurance of such rank conditions, and the related problem of the avoidance of spurious kinematic modes, are presented.

  10. Non Target Effect of Cry1 Ab and Cry Ab x Cry3 Bb1 Bt Transgenic Maize on Orius Insidiosus (Hemiptera: Anthocoridae) Abundance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Non-target effects of Cry1Ab x CP4 EPSPS and Cry1Ab + Cry3Bb1 x CP4 EPSPS Bt transgenic new maize hybrids on insidious flower bugs [Orius insidiosus (Say)] was studied in Nebraska (Mead, C lay Center, and Concord) during 2007 and 2008. The Bt effect was compared to CP4 EPSPS maize (isoline), convent...

  11. A 16-amino acid peptide from human alpha2-macroglobulin binds transforming growth factor-beta and platelet-derived growth factor-BB.

    PubMed Central

    Webb, D. J.; Roadcap, D. W.; Dhakephalkar, A.; Gonias, S. L.

    2000-01-01

    Alpha2-macroglobulin (alpha2M) is a major carrier of transforming growth factor-beta (TGF-beta) in vitro and in vivo. By screening glutathione S-transferase (GST) fusion proteins with overlapping sequences, we localized the TGFbeta-binding site to aa 700-738 of the mature human alpha2M subunit. In separate experiments, we screened overlapping synthetic peptides corresponding to aa 696-777 of alpha2M and identified a single 16-mer (718-733) that binds TGF-beta1. Platelet-derived growth factor-BB (PDGF-BB) bound to the same peptide, even though TGF-beta and PDGF-BB share almost no sequence identity. The sequence of the growth factor-binding peptide, WDLVVVNSAGVAEVGV, included a high proportion of hydrophobic amino acids. The analogous peptide from murinoglobulin, a human alpha2M homologue that does not bind growth factors, contained only three nonconservative amino acid substitutions; however, the MUG peptide failed to bind TGF-beta1 and PDGF-BB. These results demonstrate that a distinct and highly-restricted site in alpha2M, positioned near the C-terminal flank of the bait region, mediates growth factor binding. At least part of the growth factor-binding site is encoded by exon 18 of the alpha2M gene, which is notable for a 5' splice site polymorphism that has been implicated in Alzheimer's Disease. PMID:11106172

  12. Evodiamine inhibits PDGF-BB-induced proliferation of rat vascular smooth muscle cells through the suppression of cell cycle progression and oxidative stress

    PubMed Central

    Ge, Xie; Chen, Si-Yu; Liu, Mei; Liang, Ting-Ming; Liu, Chang

    2016-01-01

    Vascular smooth muscle cell (VSMC) proliferation is a key event in the development of in-stent restenosis. Evodiamine is an indole alkaloid extracted from the Chinese medicine, evodia, and has been shown to inhibit tumor cell proliferation and protect the cardiovascular system. However, whether evodiamine affects VSMC proliferation remains to be elucidated. Therefore, the present study examined the effects and the mechanisms of action of evodiamine on the proliferation of rat VSMCs. The cells were treated with evodiamine alone or in combination with platelet-derived growth factor-BB (PDGF-BB) stimulation. It was found that evodiamine inhibited PDGF-BB-induced VSMC proliferation in a dose-dependent manner, without inducing cell death. Evodiamine also retarded cell cycle progression, evidenced by the suppression of the expression of cell cycle-promoting cyclin proteins and cyclin-dependent kinases. In addition, evodiamine attenuated the PDGF-BB-induced phosphorylation of mitogen-activated protein kinases p38 and extracellular signal-regulated kinases 1/2, however, it had no effect on the phosphorylation of Akt. Evodiamine also inhibited the increase of reactive oxygen species generation and upregulated the mRNA expression levels of genes encoding antioxidant enzymes. These findings provide important insights into the mechanisms underlying the vasoprotective actions of evodiamine and suggest that it may be a useful therapeutic agent for the treatment of vascular occlusive disease. PMID:27748810

  13. Genetically modified human placenta‑derived mesenchymal stem cells with FGF‑2 and PDGF‑BB enhance neovascularization in a model of hindlimb ischemia.

    PubMed

    Yin, Tao; He, Sisi; Su, Chao; Chen, Xiancheng; Zhang, Dongmei; Wan, Yang; Ye, Tinghong; Shen, Guobo; Wang, Yongsheng; Shi, Huashan; Yang, Li; Wei, Yuquan

    2015-10-01

    Ischemic diseases represent a challenging worldwide health burden. The current study investigated the therapeutic potential of genetically modified human placenta‑derived mesenchymal stem cells (hPDMSCs) with basic fibroblast growth factor (FGF2) and platelet‑derived growth factor‑BB (PDGF‑BB) genes in hindlimb ischemia. Mesenchymal stem cells obtained from human term placenta were transfected ex vivo with adenoviral bicistronic vectors carrying the FGF2 and PDGF‑BB genes (Ad‑F‑P). Unilateral hindlimb ischemia was surgically induced by excision of the right femoral artery in New Zealand White rabbits. Ad‑F‑P genetically modified hPDMSCs, Ad‑null (control vector)‑modified hPDMSCs, unmodified hPDMSCs or media were intramuscularly implanted into the ischemic limbs 7 days subsequent to the induction of ischemia. Four weeks after cell therapy, angiographic analysis revealed significantly increased collateral vessel formation in the Ad‑F‑P‑hPDMSC group compared with the control group. Histological examination revealed markedly increased capillary and arteriole density in the Ad‑F‑P‑hPDMSC group. The xenografted hPDMSCs survived in the ischemic tissue for at least 4 weeks subsequent to cell therapy. The current study demonstrated that the combination of hPDMSC therapy with FGF2 and PDGF‑BB gene therapy effectively induced collateral vessel formation and angiogenesis, suggesting a novel strategy for therapeutic angiogenesis.

  14. A coleopteran cadherin fragment synergizes toxicity of Bacillus thuringiensis toxins Cry3Aa, Cry3Bb, and Cry8Ca against lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae).

    PubMed

    Park, Youngjin; Hua, Gang; Taylor, Milton D; Adang, Michael J

    2014-11-01

    The lesser mealworm, Alphitobius diaperinus, is a serious cosmopolitan pest of commercial poultry facilities because of its involvement in structural damage to poultry houses, reduction in feed conversion efficiency, and transfer of avian and human pathogens. Cry3Aa, Cry3Bb, and Cry8Ca insecticidal proteins of Bacillus thuringiensis are used to control coleopteran larvae. Cadherins localized in the midgut epithelium function as receptors for Cry toxins in lepidopteran, coleopteran, and dipteran insects. Previously, we demonstrated that the truncated cadherin (DvCad1) from Diabrotica virgifera virgifera, which consists of the C-terminal cadherin repeats (CR) 8-10 and expressed in Escherichia coli, enhanced Cry3Aa and Cry3Bb toxicity against several coleopteran species. Here we report that the DvCad1-CR8-10 enhances Cry3Aa, Cry3Bb, and Cry8Ca toxicity to lesser mealworm. Previously, by an enzyme linked immunosorbent microplate assay, we demonstrated that the DvCad1-CR8-10 binds activated-Cry3Aa (11.8 nM), -Cry3Bb (1.4nM), and now report that CR8-10 binds activated-Cry8Ca (5.7 nM) toxin. The extent of Cry toxins enhancement by DvCad1-CR8-10, which ranged from 3.30- to 5.93-fold, may have practical application for lesser mealworm control in preventing avian and human pathogen transfer in poultry facilities.

  15. The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and -BB and inhibits the binding of PDGF to its receptors

    SciTech Connect

    Raines, E.W.; Lane, T.F.; Iruela-Arispe, M.L.; Ross, R.; Sage, E.H. )

    1992-02-15

    Interactions among growth factors, cells, and extracellular matrix are critical to the regulation of directed cell migration and proliferation associated with development wound healing, and pathologic processes. Here the authors report the association of PDGF-AB and -BB, but not PDGF-AA, with the extracellular glycoprotein SPARC. Complexes of SPARC and {sup 125}I-labeled PDGF-BB or -AB were specifically immunoprecipitated by anti-SPARC immunoglobulins. {sup 125}I-PDGF-BB and -AB also bound specifically to SPARC that was immobilized on microtiter wells or bound to nitrocellulose after transfer from SDS/polyacrylamide gels. The binding of PDGF-BB to SPARC was pH-dependent; significant binding was detectable only above pH 6.6. Enhanced expression of both PDGF-B chain and SPARC was seen in advanced lesions of atherosclerosis. They suggest that the coordinate expression of SPARC and PDGF-B-containing dimers following vascular injury may regulate the activity of specific dimeric forms of PDGF in vivo.

  16. A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex.

    PubMed

    Lu, Lihua; Mao, Zhifeng; Kang, Tian-Shu; Leung, Chung-Hang; Ma, Dik-Lung

    2016-11-15

    The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300pM (R(2)=0.992), with a limit of detection for PDGF-BB of 10pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.

  17. In vitro evaluation of gastrointestinal survival of Lactobacillus amylovorus DSM 16698 alone and combined with galactooligosaccharides, milk and/or Bifidobacterium animalis subsp. lactis Bb-12.

    PubMed

    Martinez, Rafael C R; Aynaou, Azz-Eddine; Albrecht, Simone; Schols, Henk A; De Martinis, Elaine C P; Zoetendal, Erwin G; Venema, Koen; Saad, Susana M I; Smidt, Hauke

    2011-09-15

    Probiotic properties of Lactobacillus amylovorus DSM 16698 were previously demonstrated in piglets. Here, its potential as a human probiotic was studied in vitro, using the TIM-1 system, which is fully validated to simulate the human upper gastrointestinal tract. To evaluate the effect of the food matrix composition on the survival of L. amylovorus DSM 16698 in TIM-1, the microorganism was inoculated alone or with prebiotic galactooligosaccharides (GOS), partially skimmed milk (PSM) and/or commercial probiotic Bifidobacterium animalis subsp. lactis Bb-12 (Bb-12). Samples were collected from TIM-1 for six hours, at one-hour intervals and L. amylovorus populations were enumerated on MRS agar plates with confirmation of identity of selected isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting. The cumulative survival for L. amylovorus alone (control) was 30% at the end of the experiment (t=6h). Co-administration of L. amylovorus with GOS, PSM and/or Bb-12 increased its survival in comparison with the control significantly from the 4th hour after ingestion onwards (P<0.05). Furthermore, by the use of High Performance Anion Exchange Chromatography, both L. amylovorus and Bb-12 were observed to promptly degrade GOS compounds in samples collected from TIM-1, as assessed at t=2h. Hence, food matrix composition interfered with survival and growth of L. amylovorus during passage through TIM-1, providing leads towards optimization of probiotic properties in vivo.

  18. Selection for Resistance to the Cry3Bb1 Protein in a Genetically Diverse Population of Non-diapausing Western Corn Rootworm

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The western corn rootworm (Diabrotica virgifera virgifera) is an economically important pest of maize in North America. In 2003, the U. S. Environmental Protection Agency approved the commercial use of Monsanto’s Bt maize expressing the Cry3Bb1 protein, which is toxic to pest Diabrotica beetles. How...

  19. Baccalaureate and Beyond: A First Look at the Employment Experiences and Lives of College Graduates, 4 Years On (B&B:08/12). NCES 2014-141

    ERIC Educational Resources Information Center

    Cataldi, Emily Forrest; Siegel, Peter; Shepherd, Bryan; Cooney, Jennifer

    2014-01-01

    This report presents initial findings about the employment outcomes of bachelor's degree recipients approximately 4 years after they completed their 2007-08 degrees. These findings are based on data from the second follow-up of the Baccalaureate and Beyond Longitudinal Study (B&B:08/12), a nationally representative longitudinal sample survey…

  20. Selection for Cry3Bb1 resistance in a genetically diverse population of non-diapausing western corn rootworm (Coleoptera: Chrysomelidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Five short-diapause laboratory lines of western corn rootworm (Diabrotica virgifera virgifera) were selected for resistance to MON863, a variety of corn genetically modified with the Bacillus thuringiensis (Bt) transgene that expresses the Cry3Bb1 d-endotoxin. Three of the selected lines were develo...

  1. 2008/12 Baccalaureate and Beyond Longitudinal Study (B&B:08/12). Data File Documentation. NCES 2015-141

    ERIC Educational Resources Information Center

    Cominole, Melissa; Shepherd, Bryan; Siegel, Peter

    2015-01-01

    This publication describes the methods and procedures used in the 2008/12 Baccalaureate and Beyond Longitudinal Study (B&B:08/12). These graduates, who completed the requirements for a bachelor's degree during the 2007-08 academic year, were first interviewed as part of the 2008 National Postsecondary Student Aid Study (NPSAS:08), and then…

  2. Targeting the PDGF-B/PDGFR-β Interface with Destruxin A5 to Selectively Block PDGF-BB/PDGFR-ββ Signaling and Attenuate Liver Fibrosis.

    PubMed

    Wang, Xingqi; Wu, Xuefeng; Zhang, Aihua; Wang, Shiyu; Hu, Chunhui; Chen, Wei; Shen, Yan; Tan, Renxiang; Sun, Yang; Xu, Qiang

    2016-05-01

    PDGF-BB/PDGFR-ββ signaling plays very crucial roles in the process of many diseases such as liver fibrosis. However, drug candidates with selective affinities for PDGF-B/PDGFR-β remain deficient. Here, we identified a natural cyclopeptide termed destruxin A5 that effectively inhibits PDGF-BB-induced PDGFR-β signaling. Interestingly and importantly, the inhibitory mechanism is distinct from the mechanism of tyrosine kinase inhibitors because destruxin A5 does not have the ability to bind to the ATP-binding pocket of PDGFR-β. Using Biacore T200 technology, thermal shift technology, microscale thermophoresis technology and computational analysis, we confirmed that destruxin A5 selectively targets the PDGF-B/PDGFR-β interaction interface to block this signaling. Additionally, the inhibitory effect of destruxin A5 on PDGF-BB/PDGFR-ββ signaling was verified using in vitro, ex vivo and in vivo models, in which the extent of liver fibrosis was effectively alleviated by destruxin A5. In summary, destruxin A5 may represent an efficacious and more selective inhibitor of PDGF-BB/PDGFR-ββ signaling.

  3. 1993/03 Baccalaureate and Beyond Longitudinal Study (B&B:93/03) Field Test Methodology Report. Working Paper Series. NCES 2004-02

    ERIC Educational Resources Information Center

    Wine, J.; Cominole, M.; Carwile, S.; Franklin, J.; Carley-Baxter, L.; and Wheeless, S.

    2004-01-01

    The 1993/03 Baccalaureate and Beyond Longitudinal Study (B&B:93/03), sponsored by the National Center for Education Statistics (NCES), U.S. Department of Education, followed a cohort of students who earned bachelor's degrees during the 1992?93 academic year. These students were first interviewed in 1993, as part of the 1993 National Postsecondary…

  4. Cross-feeding between Bifidobacterium longum BB536 and acetate-converting, butyrate-producing colon bacteria during growth on oligofructose.

    PubMed

    Falony, Gwen; Vlachou, Angeliki; Verbrugghe, Kristof; De Vuyst, Luc

    2006-12-01

    In vitro coculture fermentations of Bifidobacterium longum BB536 and two acetate-converting, butyrate-producing colon bacteria, Anaerostipes caccae DSM 14662 and Roseburia intestinalis DSM 14610, with oligofructose as the sole energy source, were performed to study interspecies interactions. Two clearly distinct types of cross-feeding were identified. A. caccae DSM 14662 was not able to degrade oligofructose but could grow on the fructose released by B. longum BB536 during oligofructose breakdown. R. intestinalis DSM 14610 could degrade oligofructose, but only after acetate was added to the medium. Detailed kinetic analyses of oligofructose breakdown by the last strain revealed simultaneous degradation of the different chain length fractions, in contrast with the preferential degradation of shorter fractions by B. longum BB536. In a coculture of both strains, initial oligofructose degradation and acetate production by B. longum BB536 took place, which in turn also allowed oligofructose breakdown by R. intestinalis DSM 14610. These and similar cross-feeding mechanisms could play a role in the colon ecosystem and contribute to the combined bifidogenic/butyrogenic effect observed after addition of inulin-type fructans to the diet.

  5. Left-right asymmetric expression of BbPtx, a Ptx-related gene, in a lancelet species and the developmental left-sidedness in deuterostomes.

    PubMed

    Yasui, K; Zhang, S; Uemura, M; Saiga, H

    2000-01-01

    The long-standing question of how asymmetric development or asymmetric body structures in lancelets (amphioxus) are phylogenetically related to the body plan of other animals is still untouched. Three anterior structures, the preoral pit, club-shaped gland and mouth, are remarkable asymmetric features in developing lancelets that all open on the left side of the body. A Ptx-related gene, BbPtx is the first identified transcription factor gene with an asymmetrical expression pattern in lancelets similar to that in vertebrates, and thus it may provide a clue for the above question. Expression of the BbPtx gene is first detected at the dorsal margin of the blastopore in early mid-gastrulae and then becomes restricted to the left anterodorsal wall of the primitive gut and to the developing left somitocoelomic system. Expression continues on the left side in the developing preoral pit, club-shaped gland and mouth as well as in the mesoderm at the caudal end. Unlike D-Ptx1 in Drosophila, BbPtx is not coexpressed with a fork head gene in lancelets; instead the two genes are expressed in a complementary fashion on the left side of the embryo. The expression pattern of BbPtx is not compatible with the calcichordate hypothesis of Jefferies, in which the proposed ancestor of chordates rotated its tail 90 degrees counterclockwise in relation to the head/trunk. The expression of both BbPtx and vertebrate Pitx2 in tissues derived from the coelom implies that the left-right asymmetric development has a common origin between cephalochordates and vertebrates. Considering the development of the coelom in deuterostomes, however, left-right asymmetric development involving Pitx2-related genes is rather likely to be a primitive character shared among deuterostomes.

  6. Differential impact of CD27 and 4-1BB costimulation on effector and memory CD8 T cell generation following peptide immunization.

    PubMed

    Willoughby, Jane E; Kerr, Jonathan P; Rogel, Anne; Taraban, Vadim Y; Buchan, Sarah L; Johnson, Peter W M; Al-Shamkhani, Aymen

    2014-07-01

    The factors that determine differentiation of naive CD8 T cells into memory cells are not well understood. A greater understanding of how memory cells are generated will inform of ways to improve vaccination strategies. In this study, we analyzed the CD8 T cell response elicited by two experimental vaccines comprising a peptide/protein Ag and an agonist that delivers a costimulatory signal via CD27 or 4-1BB. Both agonists increased expansion of Ag-specific CD8 T cells compared with Ag alone. However, their capacity to stimulate differentiation into effector and memory cells differed. CD27 agonists promoted increased expression of perforin and the generation of short-lived memory cells, whereas stimulation with 4-1BB agonists favored generation of stable memory. The memory-promoting effects of 4-1BB were independent of CD4 T cells and were the result of programing within the first 2 d of priming. Consistent with this conclusion, CD27 and 4-1BB-stimulated CD8 T cells expressed disparate amounts of IL-2, IFN-γ, CD25, CD71, and Gp49b as early as 3 d after in vivo activation. In addition, memory CD8 T cells, generated through priming with CD27 agonists, proliferated more extensively than did 4-1BB-generated memory cells, but these cells failed to persist. These data demonstrate a previously unanticipated link between the rates of homeostatic proliferation and memory cell attrition. Our study highlights a role for these receptors in skewing CD8 T cell differentiation into effector and memory cells and provides an approach to optimize vaccines that elicit CD8 T cell responses.

  7. Ellagic acid inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and prevents atheroma formation in streptozotocin-induced diabetic rats.

    PubMed

    Rani, Uma P; Kesavan, Rushendhiran; Ganugula, Raghu; Avaneesh, T; Kumar, Uday P; Reddy, G Bhanuprakash; Dixit, Madhulika

    2013-11-01

    Plant-derived polyphenolic compounds have beneficial health effects. In the present study, we determined the ability of ellagic acid (EA) to prevent platelet-derived growth factor-BB (PDGF-BB)-induced proliferation of primary cultures of rat aortic smooth muscle cells (RASMCs). We also determined the ability of EA to prevent atherosclerosis in streptozotocin-induced diabetic rats. Proliferation of cells was measured via Alamar Blue assay and through propidium iodide-based cell cycle analysis in flow cytometer. Reactive oxygen species (ROS) were measured via 2',7'-dichlorofluorescin diacetate and Amplex red methods. Expression of proliferation markers and activation of kinases were assessed by immunoblot analysis. Cotreatment of primary cultures of RASMCs with 25 μmol/L of EA significantly reduced PDGF-BB (20 ng/ml)-induced proliferation by blocking S-phase entry. EA effectively blocked PDGF receptor-β (PDGFR-β) tyrosine phosphorylation, generation of intracellular ROS and downstream activation of extracellular signal-regulated kinase 1/2. It also blocked PDGF-BB-induced expression of cyclin D1. Computational molecular docking of EA with the PDGFR-β-PDGF-BB complex revealed two putative inhibitor binding sites which showed similar binding energies with the known PDGFR-β inhibitor AG1295. In diabetic rats, supplementation of diet with 2% EA significantly blocked diabetes-induced medial thickness, and lipid and collagen deposition in the arch of aorta. These were assessed through haematoxylin and eosin, Oil Red O and Masson's trichome staining, respectively. EA treatment also blocked cyclin D1 expression in medial smooth muscle cells in experimental animals. Thus, EA is effective in reducing atherosclerotic process by blocking proliferation of vascular smooth muscle cells.

  8. Protein-templated cobaltous phosphate nanocomposites for the highly sensitive and selective detection of platelet-derived growth factor-BB.

    PubMed

    He, Linghao; Zhang, Shuai; Ji, Hongfei; Wang, Minghua; Peng, Donglai; Yan, Fufeng; Fang, Shaoming; Zhang, Hongzhong; Jia, Chunxiao; Zhang, Zhihong

    2016-05-15

    We synthesized novel Co3(PO4)2-based nanocomposites with 3D porous architectures via self-assembly; here, bovine serum albumin (BSA) and aptamer were used as organic phases to produce Co3(PO4)2@BSA and Co3(PO4)2@Apt nanocomposites, respectively. The formation mechanism of Co3(PO4)2-based nanocomposites was described based on characterizations of their physio-chemical performance, and the developed nanocomposites were applied as scaffold materials to construct a novel electrochemical aptasensor and detect platelet-derived growth factor-BB (PDGF-BB). The PDGF-BB targeting aptamer must be immobilized onto the Co3(PO4)2@BSA-modified electrode to detect PDGF-BB, whereas Co3(PO4)2@Apt-based aptasensor may be directly used to determine the target protein. Electrochemical impedance spectroscopy results showed that the developed Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors present highly sensitive detection ability toward PDGF-BB. Due to the special nanoflower structure, the Co3(PO4)2@BSA-based aptasensor features a detection limit of 3.7 pg mL(-1); while the limit of detection of the Co3(PO4)2@Apt-based aptasensor is 61.5 pg mL(-1), which is the possible bioactivity loss of the aptamer in Co3(PO4)2@Apt nanocomposite. The two detection limits obtained are still much lower than or comparable with those of previously reported aptasensors. The Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors showed high selectivity, stability, and applicability for detecting the desired protein. This finding indicates that the Co3(PO4)2-based nanocomposites could be used as an electrochemical biosensor for various detection procedures in the biomedical field.

  9. The site-directed mutation I(L177)H in Rhodobacter sphaeroides reaction center affects coordination of P(A) and B(B) bacteriochlorophylls.

    PubMed

    Vasilieva, L G; Fufina, T Y; Gabdulkhakov, A G; Leonova, M M; Khatypov, R A; Shuvalov, V A

    2012-08-01

    To explore the influence of the I(L177)H single mutation on the properties of the nearest bacteriochlorophylls (BChls), three reaction centers (RCs) bearing double mutations were constructed in the photosynthetic purple bacterium Rhodobacter sphaeroides, and their properties and pigment content were compared with those of the correspondent single mutant RCs. Each pair of the mutations comprised the amino acid substitution I(L177)H and another mutation altering histidine ligand of BChl P(A) or BChl B(B). Contrary to expectations, the double mutation I(L177)H+H(L173)L does not bring about a heterodimer RC but causes a 46nm blue shift of the long-wavelength P absorbance band. The histidine L177 or a water molecule were suggested as putative ligands for P(A) in the RC I(L177)H+H(L173)L although this would imply a reorientation of the His backbone and additional rearrangements in the primary donor environment or even a repositioning of the BChl dimer. The crystal structure of the mutant I(L177)H reaction center determined to a resolution of 2.9Å shows changes at the interface region between the BChl P(A) and the monomeric BChl B(B). Spectral and pigment analysis provided evidence for β-coordination of the BChl B(B) in the double mutant RC I(L177)H+H(M182)L and for its hexacoordination in the mutant reaction center I(L177)H. Computer modeling suggests involvement of two water molecules in the β-coordination of the BChl B(B). Possible structural consequences of the L177 mutation affecting the coordination of the two BChls P(A) and B(B) are discussed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.

  10. Platelet-derived growth factor-BB induces cystathionine γ-lyase expression in rat mesangial cells via a redox-dependent mechanism

    PubMed Central

    Hassan, Mohamed I; Boosen, Meike; Schaefer, Liliana; Kozlowska, Jowita; Eisel, Florian; von Knethen, Andreas; Beck, Martina; Hemeida, Ramadan A M; El-Moselhy, Mohamed A M; Hamada, Farid M A; Beck, Karl-Friedrich; Pfeilschifter, Josef

    2012-01-01

    BACKGROUND AND PURPOSE So far, there is only limited information about the regulation of the endogenous synthesis of hydrogen sulfide (H2S), an important gaseous signalling molecule. This study was done to evaluate the redox-dependent signalling events that regulate the expression of the H2S synthesising enzyme cystathionine-γ-lyase (CSE) in rat mesangial cells. EXPERIMENTAL APPROACH The effects of platelet-derived growth factor (PDGF)-BB and antioxidants on CSE expression and activity in cultured rat renal mesangial cells were assessed. Activity of nuclear factor erythroid-2-related factor-2 (Nrf2) was measured as the binding capacity to a radiolabelled consensus element by electrophoretic mobility shift assay (EMSA). Furthermore, CSE and Nrf2 expression was analysed in a rat model of anti-Thy-1-induced glomerulonephritis by immunohistochemistry. KEY RESULTS Treatment of mesangial cells with PDGF-BB resulted in a marked time- and dose-dependent up-regulation of CSE mRNA and protein levels, as well as CSE activity accompanied with increased formation of reactive oxygen species. Remarkably, co-administration of antioxidants, such as N-acetylcysteine, ebselen or diphenylene iodonium chloride, drastically reduced PDGF-BB-induced CSE expression. PDGF-BB induced binding of Nrf2 to a corresponding consensus antioxidant element in a redox-dependent manner. Furthermore, PDGF-BB-induced CSE expression in mouse mesangial cells was completely abolished in Nrf2 knockout mice compared with wild-type mice. In a rat model of anti-Thy-1-induced proliferative glomerulonephritis, we observed a marked up-regulation of CSE protein paralleled by a stabilization of Nrf2 protein. CONCLUSIONS AND IMPLICATIONS PDGF-BB regulated CSE via a redox-mediated activation of Nrf2. Such action would aid the resolution of glomerular inflammatory diseases. LINKED ARTICLE This article is commented on by Gallyas, pp. 2228–2230 of this issue. To view this commentary visit http://dx.doi.org/10.1111/j

  11. The type Ia supernova SNLS-03D3bb from a super-Chandrasekhar-mass white dwarf star.

    PubMed

    Howell, D Andrew; Sullivan, Mark; Nugent, Peter E; Ellis, Richard S; Conley, Alexander J; Le Borgne, Damien; Carlberg, Raymond G; Guy, Julien; Balam, David; Basa, Stephane; Fouchez, Dominique; Hook, Isobel M; Hsiao, Eric Y; Neill, James D; Pain, Reynald; Perrett, Kathryn M; Pritchet, Christopher J

    2006-09-21

    The accelerating expansion of the Universe, and the need for dark energy, were inferred from observations of type Ia supernovae. There is a consensus that type Ia supernovae are thermonuclear explosions that destroy carbon-oxygen white dwarf stars that have accreted matter from a companion star, although the nature of this companion remains uncertain. These supernovae are thought to be reliable distance indicators because they have a standard amount of fuel and a uniform trigger: they are predicted to explode when the mass of the white dwarf nears the Chandrasekhar mass of 1.4 solar masses (M(o)). Here we show that the high-redshift supernova SNLS-03D3bb has an exceptionally high luminosity and low kinetic energy that both imply a super-Chandrasekhar-mass progenitor. Super-Chandrasekhar-mass supernovae should occur preferentially in a young stellar population, so this may provide an explanation for the observed trend that overluminous type Ia supernovae occur only in 'young' environments. As this supernova does not obey the relations that allow type Ia supernovae to be calibrated as standard candles, and as no counterparts have been found at low redshift, future cosmology studies will have to consider possible contamination from such events.

  12. A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing.

    PubMed

    Prapa, Malvina; Caldrer, Sara; Spano, Carlotta; Bestagno, Marco; Golinelli, Giulia; Grisendi, Giulia; Petrachi, Tiziana; Conte, Pierfranco; Horwitz, Edwin M; Campana, Dario; Paolucci, Paolo; Dominici, Massimo

    2015-09-22

    Chimeric antigen receptor (CAR)-expressing T cells are a promising therapeutic option for patients with cancer. We developed a new CAR directed against the disialoganglioside GD2, a surface molecule expressed in neuroblastoma and in other neuroectoderm-derived neoplasms. The anti-GD2 single-chain variable fragment (scFv) derived from a murine antibody of IgM class was linked, via a human CD8α hinge-transmembrane domain, to the signaling domains of the costimulatory molecules 4-1BB (CD137) and CD3-ζ. The receptor was expressed in T lymphocytes by retroviral transduction and anti-tumor activities were assessed by targeting GD2-positive neuroblastoma cells using in vitro cytotoxicity assays and a xenograft model. Transduced T cells expressed high levels of anti-GD2 CAR and exerted a robust and specific anti-tumor activity in 4- and 48-hour cultures with neuroblastoma cells. Cytotoxicity was associated with the release of pro-apoptotic molecules such as TRAIL and IFN-γ. These results were confirmed in a xenograft model, where anti-GD2 CAR T cells infiltrating tumors and persisting into blood circulation induced massive apoptosis of neuroblastoma cells and completely abrogated tumor growth. This anti-GD2 CAR represents a powerful new tool to redirect T cells against GD2. The preclinical results of this study warrant clinical testing of this approach in neuroblastoma and other GD2-positive malignancies.

  13. The type Ia supernova SNLS-03D3bb from a super-Chandrasekhar-masswhite dwarf star

    SciTech Connect

    Howell, D.Andrew; Sullivan, Mark; Nugent, Peter E.; Ellis,Richard S.; Conley, Alexander J.; Le Borgne, Damien; Carlberg, RaymondG.; Guy, Julien; Balam, David; Basa, Stephane; Fouchez, Dominique; Hook,Isobel M.; Hsiao, Eric Y.; Neill, James D.; Pain, Reynald; Perrett,Kathryn M.; Pritchet, Christopher J.

    2006-02-01

    The acceleration of the expansion of the universe, and theneed for Dark Energy, were inferred from the observations of Type Iasupernovae (SNe Ia) 1;2. There is consensus that SNeIa are thermonuclearexplosions that destroy carbon-oxygen white dwarf stars that accretematter from a companion star3, although the nature of this companionremains uncertain. SNe Ia are thought to be reliable distance indicatorsbecause they have a standard amount of fuel and a uniform trigger theyare predicted to explode when the mass of the white dwarf nears theChandrasekhar mass 4 - 1.4 solar masses. Here we show that the highredshift supernova SNLS-03D3bb has an exceptionally high luminosity andlow kinetic energy that both imply a super-Chandrasekhar mass progenitor.Super-Chandrasekhar mass SNeIa shouldpreferentially occur in a youngstellar population, so this may provide an explanation for the observedtrend that overluminous SNe Ia only occur in young environments5;6. Sincethis supernova does not obey the relations that allow them to becalibrated as standard candles, and since no counterparts have been foundat low redshift, future cosmology studies will have to considercontamination from such events.

  14. Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC).

    PubMed

    Yamagishi, Hiroshi; Tanaka, Yoshiyuki; Terachi, Toru

    2014-11-01

    Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.

  15. PI3K is involved in PDGF-beta receptor upregulation post-PDGF-BB treatment in mouse HSC.

    PubMed

    Lechuga, Carmen G; Hernández-Nazara, Zamira H; Hernández, Elizabeth; Bustamante, Marcia; Desierto, Gregory; Cotty, Adam; Dharker, Nachiket; Choe, Moran; Rojkind, Marcos

    2006-12-01

    Increased expression of PDGF-beta receptors is a landmark of hepatic stellate cell activation and transdifferentiation into myofibroblasts. However, the molecular mechanisms that regulate the fate of the receptor are lacking. Recent studies suggested that N-acetylcysteine enhances the extracellular degradation of PDGF-beta receptor by cathepsin B, thus suggesting that the absence of PDGF-beta receptors in quiescent cells is due to an active process of elimination and not to a lack of expression. In this communication we investigated further molecular mechanisms involved in PDGF-beta receptor elimination and reappearance after incubation with PDGF-BB. We showed that in culture-activated hepatic stellate cells there is no internal protein pool of receptor, that the protein is maximally phosphorylated by 5 min and completely degraded after 1 h by a lysosomal-dependent mechanism. Inhibition of receptor autophosphorylation by tyrphostin 1296 prevented its degradation, but several proteasomal inhibitors had no effect. We also showed that receptor reappearance is time and dose dependent, being more delayed in cells treated with 50 ng/ml (48 h) compared with 10 ng/ml (24 h).

  16. 4-1BB Costimulation Ameliorates T Cell Exhaustion Induced by Tonic Signaling of Chimeric Antigen Receptors

    PubMed Central

    Long, Adrienne H.; Haso, Waleed M.; Shern, Jack F.; Wanhainen, Kelsey M.; Murgai, Meera; Ingaramo, Maria; Smith, Jillian P.; Walker, Alec J.; Kohler, M. Eric; Venkateshwara, Vikas R.; Kaplan, Rosandra N.; Patterson, George H.; Fry, Terry J.; Orentas, Rimas J.; Mackall, Crystal L.

    2015-01-01

    Chimeric antigen receptors (CARs) targeting CD19 have mediated dramatic anti-tumor responses in hematologic malignancies, but tumor regression has rarely occurred using CARs targeting other antigens. It remains unknown whether the impressive effects of CD19 CARs relate to greater susceptibility of hematologic malignancies to CAR therapies, or superior functionality of the CD19 CAR itself. We discovered that tonic CAR CD3ζ phosphorylation, triggered by antigen-independent clustering of CAR scFvs, can induce early exhaustion of CAR T cells that limits anti-tumor efficacy. Such activation is present to varying degrees in all CARs studied, with the exception of the highly effective CD19 CAR. We further identify that CD28 costimulation augments, while 4-1BB costimulation ameliorates, exhaustion induced by persistent CAR signaling. Our results provide biological explanations for the dramatic anti-tumor effects of CD19 CARs and for the observations that CD19.BBz CAR T cells are more persistent than CD19.28z CAR T cells in clinical trials. PMID:25939063

  17. Mammea E/BB, an isoprenylated dihydroxycoumarin protonophore that potently uncouples mitochondrial electron transport, disrupts hypoxic signaling in tumor cells.

    PubMed

    Du, Lin; Mahdi, Fakhri; Jekabsons, Mika B; Nagle, Dale G; Zhou, Yu-Dong

    2010-11-29

    The mammea-type coumarin mammea E/BB (1) was found to inhibit both hypoxia-induced and iron chelator-induced hypoxia-inducible factor-1 (HIF-1) activation in human breast tumor T47D cells with IC(50) values of 0.96 and 0.89 μM, respectively. Compound 1 suppressed the hypoxic induction of secreted VEGF protein (T47D cells) and inhibited cell viability/proliferation in four human tumor cell lines. Compound 1 (at 5 and 20 μM) inhibited human breast tumor MDA-MB-231 cell migration. While the mechanisms that underlie their biological activities have remained unknown, prenylated mammea coumarins have been shown to be cytotoxic to human tumor cells, suppress tumor growth in animal models, and display a wide variety of antimicrobial effects. Mechanistic studies revealed that 1 appears to exert an assemblage of cellular effects by functioning as an anionic protonophore that potently uncouples mitochondrial electron transport and disrupts mitochondrial signaling in human tumor cell lines.

  18. Effects of (-) mammea A/BB isolated from Calophyllum brasiliense leaves and derivatives on mitochondrial membrane of Leishmania amazonensis.

    PubMed

    Brenzan, M A; Santos, A O; Nakamura, C V; Filho, B P Dias; Ueda-Nakamura, T; Young, M C M; Côrrea, A G; Júnior, J Alvim; Morgado-Díaz, J A; Cortez, D A G

    2012-02-15

    We have previously demonstrated antileishmanial activity on Leishmania amazonensis of the natural (1-2), synthetic (7) and derivatives of coumarin (-) mammea A/BB (3-6) isolated from the dichloromethane extract of Calophyllum brasiliense leaves. The aim of the present study was to evaluate morphological and ultrastructural alterations in Leishmania amazonensis induced by these compounds. In promastigote forms, all seven compounds produced significant morphological and ultrastructural alterations, as revealed by scanning and transmission electron microscopy. The compound 5,7-dihydroxy-8-(2-methylbutanoyl)-6-(3-methylbutyl)-4-phenyl-chroman-2-one (3), the most active antileishmanial with LD₅₀ of 0.9 μM), induced cell shrinkage and a rounded appearance of the cells. Parasites incubated in the presence of compound (3) showed ultrastructural changes, such as the appearance of mitochondrial swelling with a reduction in the density of the mitochondrial matrix and the presence of vesicles inside the mitochondrion, indicating damage and significant change in this organelle; abnormal chromatin condensation, alterations in the nuclear envelope, intense atypical cytoplasmic vacuolization, and the appearance of autophagic vacuoles were also observed. In addition, the compound (3) may be acting to depolarize the mitochondrial membrane potential of the cells, leading to death of the parasite.

  19. Mammea E/BB, An Isoprenylated Dihydroxycoumarin Protonophore that Potently Uncouples Mitochondrial Electron Transport Disrupts Hypoxic Signaling in Tumor Cells

    PubMed Central

    Du, Lin; Mahdi, Fakhri; Jekabsons, Mika B.; Nagle, Dale G.; Zhou, Yu-Dong

    2010-01-01

    The mammea-type coumarin mammea E/BB (1) was found to inhibit both hypoxia-induced and iron chelator-induced hypoxia-inducible factor-1 (HIF-1) activation in human breast tumor T47D cells with IC50 values of 0.96 and 0.89 µM, respectively. Compound 1 suppressed the hypoxic induction of secreted VEGF protein (T47D cells) and inhibited cell viability/proliferation in four human tumor cell lines. Compound 1 (at 5 and 20 µM) inhibited human breast tumor MDA-MB-231 cell migration. While the mechanisms that underlay their biological activities have remained unknown, prenylated mammea coumarins have been shown to be cytotoxic to human tumor cells, suppress tumor growth in animal models, and display a wide variety of antimicrobial effects. Mechanistic studies revealed that 1 appears to exert an assemblage of cellular effects by functioning as an anionic protonophore that potently uncouples mitochondrial electron transport and disrupts mitochondrial signaling in human tumor cell lines. PMID:20929261

  20. Effect of probiotic Bifidobacterium longum BB536 [corrected] in relieving clinical symptoms and modulating plasma cytokine levels of Japanese cedar pollinosis during the pollen season. A randomized double-blind, placebo-controlled trial.

    PubMed

    Xiao, J Z; Kondo, S; Yanagisawa, N; Takahashi, N; Odamaki, T; Iwabuchi, N; Iwatsuki, K; Kokubo, S; Togashi, H; Enomoto, K; Enomoto, T

    2006-01-01

    Probiotic microorganisms have been shown to be effective in the treatment of allergic inflammation and food allergy, but their efficacy remains controversial. This study tested the effect of a yogurt supplemented with a probiotic strain Bifidobacterium longum BB536 in the treatment of Japanese cedar pollinosis (JCPsis). Forty subjects with a clinical history of JCPsis were given yoghurt either containing BB536 (BB536 yoghurt) or without BB536 (placebo yoghurt) at 2 X 100 g per day for 14 weeks, in a randomized, double-blind, placebo-controlled trial. Subjective symptoms and self-care measures were recorded daily and blood samples were taken before and during the intervention (at weeks 4, 9, and 14) to measure the blood parameter levels related to JCPsis. Yoghurt supplemented with BB536 significantly alleviated eye symptoms compared with placebo yoghurt (odds ratio 0.31; 95% confidence interval 0.10-0.97; p = 0.044). Although no statistically significant differences were detected, nasal symptoms such as itching, rhinorrhea, and blockage, as well as throat symptoms tended to be relieved with the BB536 yoghurt. BB536 tended to suppress the decreasing blood levels of interferon-gamma (IFN-y) and the increasing blood eosinophil rates; a significantly higher IFN-gamma level was observed for the difference from baseline at week 4. A decreased trend in the difference from baseline levels of JCP-specific IgE levels was also observed at week 4 in the BB536 group compared with the placebo group. In conclusion, these results suggest that intake of BB536-supplemented yoghurt may relieve JCPsis symptoms, probably through a modulating effect on Th balance.

  1. Near-resonant rovibronic Raman scattering from 0 g + ( bb) valence state via the D0 u + ion-pair state in iodine molecule

    NASA Astrophysics Data System (ADS)

    Baturo, V. V.; Cherepanov, I. N.; Lukashov, S. S.; Petrov, A. N.; Poretsky, S. A.; Pravilov, A. M.

    2016-12-01

    Near-resonant Raman scattering from the electronic excited {I_2}( {0_g^ + ( {bb} )xrightarrow{{hv}}D0_u^ + to X0_g^ + } ) state via the intermediate ion-pair D0 u + state to the X one is observed for the first time. The Raman scattering follows a laser pulse. Its intensity I R is inversely proportional to the squared value of detuning from the resonant D, 22, 51 ← 0 g +( bb), 7, 52 transition, (Δν2)2, according to the theory of near-resonant Raman scattering. The ratio of Raman D → X scattering intensity to that of the D0 u +, ν D = 22, J D = 51 → X0 g + luminescence, I R / I D‒ X < 1.5 × 10-4 for Δν2 > 0.5 cm-1. The Raman and luminescence spectra are found to be identical.

  2. BoBB, software to assess soil erosion risk - introduction of the tool and its use to evaluate appropriate crops and farming practices on endangered field plots

    NASA Astrophysics Data System (ADS)

    Devátý, Jan; Dostál, Tomáš; Hösl, Rosemarie; Strauss, Peter; Novotný, Ivan

    2013-04-01

    BoBB (Bodenerosion, Beratung, Berechnung) is simple software to support instant assessment of soil erosion hazard on agricultural fields. The program is profile-oriented, implementing the RUSLE model with slight changes allowing it to assess and compare different farming practices especially the soil-conservation field management. The input parameters datasets are supplied with necessary data for territory and natural conditions of Upper Austria but are generally usable for Central Europe. The software was developed on Federal Agency for Water Management, Petzenkirchen, Austria in 2011 - 2012. BAW and CTU in Prague are recently cooperating on validation and practical applicability approval of the model. Basic validation was done by comparing the outputs of the BoBB software with outputs of the original RUSLE model calculated by the RUSLE1 (USDA, 1998) and RUSLE2 (USDA, 2005) softwares. Further evaluation was performed to test the possibilities of BoBB to reveal field plots endangered by soil erosion. First, testing areas were selected out of a map of soil erosion risk, which had been calculated for the whole territory of the Czech Republic using a combination of the USLE approach and a GIS approach referring to the best available data set. This map in 10x10 meters resolution is used as basic source for assessment of soil erosion hazard and for necessity of GAEC requirements (Good agricultural practices assessment for agricultural subsidy policy) and is therefore accepted as standard at state level. Characteristic profiles were selected within defined testing areas and soil erosion hazard, determined by the USLE approach and BoBB have then been compared. Second, a comparison of BoBB outputs and database of soil erosion events (http://me.vumop.cz) was carried out. The database is created and maintained by the Czech Institute of Soil Conservation as a unique tool for soil erosion mapping and documentation. It was launched in 2010 and recently contains approximately

  3. Cholera toxin treatment of vascular smooth muscle cells decreases smooth muscle α-actin content and abolishes the platelet-derived growth factor-BB-stimulated DNA synthesis

    PubMed Central

    Sachinidis, Agapios; Seul, Claudia; Gouni-Berthold, Ioanna; Seewald, Stefan; Ko, Yon; Vetter, Hans; Fingerle, Jürgen; Hoppe, Jürgen

    2000-01-01

    The second messenger cyclic AMP regulates diverse biological processes such as cell morphology and cell growth. We examined the role of the second messenger cyclic AMP on rat aortic vascular smooth muscle cell (VSMC) morphology and the intracellular transduction pathway mediated by platelet-derived growth factor β-receptor (PDGF-Rβ). The effect of PDGF-BB on VSMCs growth was assessed by [3H]-thymidine incorporation. Tyrosine phosphorylation of PDGF-Rβ, PLC-γ1, ERK1 and ERK2, p125FAK and paxillin as well as Sm α-actin was examined by the chemiluminescence Western blotting method. Actin mRNA level was quantitated by Northern blotting. Visualization of Sm α-actin filaments, paxillin and PDGF-Rβ was performed by immunfluorescence microscopy. Cholera toxin (CTX; 10 nM) treatment lead to a large and sustained increase in the cyclic AMP concentration after 2 h which correlated with change of VSMC morphology including complete disruption of the Sm α-actin filament array and loss of focal adhesions. Treatment of VSMCs with CTX did not influence tyrosine phosphorylation of p125FAK and paxillin but decreased the content of a Sm α-actin protein. Maximal decrease of 70% was observed after 24 h of treatment. CTX also caused a 90% decrease of the actin mRNA level. CTX treatment completely abolished PDGF-BB stimulated DNA-synthesis although PDGF-Rβ level and subcellular distribution and translocation was not altered. Furthermore CTX attenuated the PDGF-BB-induced tyrosine phosphorylation of the PDGF-Rβ, PI 3′-K, PLC-γ1 and ERK1/2 indicating an action of cyclic AMP on PDGF-β receptor. We conclude that although cyclic AMP attenuates the PDGF-Rβ mediated intracellular transduction pathway, an intact actin filament may be required for the PDGF-BB-induced DNA synthesis in VSMCs. PMID:10928958

  4. Laboratory toxicity studies demonstrate no adverse effects of Cry1Ab and Cry3Bb1 to larvae of Adalia bipunctata (Coleoptera: Coccinellidae): the importance of study design.

    PubMed

    Alvarez-Alfageme, Fernando; Bigler, Franz; Romeis, Jörg

    2011-06-01

    Scientific studies are frequently used to support policy decisions related to transgenic crops. Schmidt et al., Arch Environ Contam Toxicol 56:221-228 (2009) recently reported that Cry1Ab and Cry3Bb were toxic to larvae of Adalia bipunctata in direct feeding studies. This study was quoted, among others, to justify the ban of Bt maize (MON 810) in Germany. The study has subsequently been criticized because of methodological shortcomings that make it questionable whether the observed effects were due to direct toxicity of the two Cry proteins. We therefore conducted tritrophic studies assessing whether an effect of the two proteins on A. bipunctata could be detected under more realistic routes of exposure. Spider mites that had fed on Bt maize (events MON810 and MON88017) were used as carriers to expose young A. bipunctata larvae to high doses of biologically active Cry1Ab and Cry3Bb1. Ingestion of the two Cry proteins by A. bipunctata did not affect larval mortality, weight, or development time. These results were confirmed in a subsequent experiment in which A. bipunctata were directly fed with a sucrose solution containing dissolved purified proteins at concentrations approximately 10 times higher than measured in Bt maize-fed spider mites. Hence, our study does not provide any evidence that larvae of A. bipunctata are sensitive to Cry1Ab and Cry3Bb1 or that Bt maize expressing these proteins would adversely affect this predator. The results suggest that the apparent harmful effects of Cry1Ab and Cry3Bb1 reported by Schmidt et al., Arch Environ Contam Toxicol 56:221-228 (2009) were artifacts of poor study design and procedures. It is thus important that decision-makers evaluate the quality of individual scientific studies and do not view all as equally rigorous and relevant.

  5. Potent costimulation of human CD8 T cells by anti-4-1BB and anti-CD28 on synthetic artificial antigen presenting cells.

    PubMed

    Rudolf, Despina; Silberzahn, Tobias; Walter, Steffen; Maurer, Dominik; Engelhard, Johanna; Wernet, Dorothee; Bühring, Hans-Jörg; Jung, Gundram; Kwon, Byoung S; Rammensee, Hans-Georg; Stevanović, Stefan

    2008-02-01

    The in vitro generation of cytotoxic T lymphocytes (CTLs) for anticancer immunotherapy is a promising approach to take patient-specific therapy from the bench to the bedside. Two criteria must be met by protocols for the expansion of CTLs: high yield of functional cells and suitability for good manufacturing practice (GMP). The antigen presenting cells (APCs) used to expand the CTLs are the key to achieving both targets but they pose a challenge: Unspecific stimulation is not feasible because only memory T cells are expanded and not rare naïve CTL precursors; in addition, antigen-specific stimulation by cell-based APCs is cumbersome and problematic in a clinical setting. However, synthetic artificial APCs which can be loaded reproducibly with MHC-peptide monomers and antibodies specific for costimulatory molecules could resolve these problems. The purpose of this study was to investigate the potential of complex synthetic artificial APCs in triggering the costimulatory molecules CD28 and 4-1BB on the T cell. Anti-4-1BB antibodies were added to an established system of microbeads coated with MHC-peptide monomers and anti-CD28. Triggering via CD28 and 4-1BB resulted in strong costimulatory synergy. The quantitative ratio between these signals determined the outcome of the stimulation with optimal results when anti-4-1BB and anti-CD28 were applied in a 3:1 ratio. Functional CTLs of an effector memory subtype (CD45RA(-) CCR7(-)) were generated in high numbers. We present a highly defined APC platform using off-the-shelf reagents for the convenient generation of large numbers of antigen-specific CTLs.

  6. Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury.

    PubMed

    Yan, Gaoliang; Wang, Qingjie; Hu, Shengda; Wang, Dong; Qiao, Yong; Ma, Genshan; Tang, Chengchun; Gu, Yuchun

    2015-10-01

    The increased proliferation and migration of vascular smooth muscle cells (VSMCs) are key events in the development of artery restenosis following percutaneous coronary intervention. Digoxin has long been used in the treatment of heart failure and has been shown to inhibit the proliferation of cancer cells through multiple pathways. However, the potential role of digoxin in the regulation of VSMC proliferation and migration and its effectiveness in the treatment of cardiovascular diseases, such as restenosis, remains unexplored. In the present study, we demonstrate that digoxin-induced growth inhibition is associated with the downregulation of CDK activation and the restoration of p27Kip1 levels in platelet-derived growth factor (PDGF)-stimulated VSMCs. In addition, we found that digoxin restored the PDGF‑BB-induced inhibition of integrin linked kinase (ILK) expression and prevented the PDGF‑BB-induced activation of glycogen synthase kinase (GSK)-3β. Furthermore, digoxin inhibited adhesion molecule and extracellular matrix relative protein expression. Finally, we found that digoxin significantly inhibited neointima formation, accompanied by a decrease in cell proliferation following vascular injury in rats. These effects of digoxin were shown to be mediated, at least in part, through an increase in ILK/Akt signaling and a decrease in GSK-3β signaling in PDGF‑BB-stimulated VSMCs. In conclusion, our data demonstrate that digoxin exerts an inhibitory effect on the PDGF‑BB-induced proliferation, migration and phenotypic modulation of VSMCs, and prevents neointima formation in rats. These observations indicate the potential therapeutic application of digoxin in the treatment of cardiovascular diseases, such as restenosis.

  7. In Vitro Effects of PDGF Isoforms (AA, BB, AB and CC) on Migration and Proliferation of SaOS-2 Osteoblasts and on Migration of Human Osteoblasts.

    PubMed

    Colciago, Alessandra; Celotti, Fabio; Casati, Lavinia; Giancola, Rinaldo; Castano, Stefano M; Antonini, Guido; Sacchi, Maria Cristina; Negri-Cesi, Paola

    2009-12-01

    PDGF is a major constituent of platelet rich plasma (PRP), responsible of chemotactic and possibly of mitogenic effects of PRP on osteoblasts. PDGF family includes 5 isoforms: PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, all expressed in platelets except PDGF-DD. Aim of this study was to analyze the effect of recombinant hPDGF-A, -AB, -B and -C, on migration and proliferation of a human osteoblastic cell line, SaOS-2. Preliminary observations on cell migration were also done in primary cultures of human osteoblasts. In vitro microchemotaxis and (3)H-thymidine mitogenic assays were used. While PDGF-AB is active at concentrations present in PRP, PDGF-AA and BB are chemotactic only at much higher doses. PDGF-C is totally inactive alone or together with the active isoforms. PDGF-AA, PDGF-BB and PDGF-C stimulate SaOS-2 proliferation only at the highest dose tested, while PDGF-AB is ineffective. Primary osteoblasts are less sensitive than SaOS-2 and progressively lose responsiveness with increasing passages in culture, in line with loss of cell differentiation. The different PDGF isoforms act differentially on osteoblasts, the-AB isoform appearing the major responsible of the PRP chemiotaxis. PDGF, at the concentrations present in PRP, does not affect cell proliferation.

  8. Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.

    PubMed

    Fujii, Takaaki; Onimaru, Mitsuho; Yonemitsu, Yoshikazu; Kuwano, Hiroyuki; Sueishi, Katsuo

    2008-06-01

    3-Hydroxy-3-methyl-glutaryl CoA reductase inhibitors, or statins, have pleiotropic effects and can protect the vasculature in a manner independent of their lipid-lowering effect. The effectiveness of statins in reducing the risk of coronary events has been shown even in patients with diabetes, and their effects on diabetic complications have been reported. Using a model of severe hindlimb ischemia in streptozotocin-induced diabetic mice (STZ-DM), we investigated the effects and mechanisms of statin therapy in diabetic angiopathy in ischemic hindlimbs. As a result, STZ-DM mice frequently lost their hindlimbs after induced ischemia, whereas non-DM mice did not. Supplementation with statins significantly prevented autoamputation. We previously showed that diabetic vascular complications are caused by impaired expression of PDGF-BB, but statin therapy did not enhance PDGF-BB expression. Statins helped enhance endogenous endothelial nitric oxide (NO) synthase (eNOS) expression. Furthermore, the inhibition of NO synthesis by the administration of N(omega)-nitro-l-arginine methyl ester impaired the ability of statins to prevent STZ-DM mouse limb autoamputation, indicating that the therapeutic effect of statins in hindlimb ischemia in STZ-DM mice occurs via the eNOS/NO pathway. A combination therapy of statins and PDGF-BB gene supplementation was more effective for diabetic angiopathy than either therapy alone. In conclusion, these findings indicate that statin therapy might be useful for preventing intractable diabetic foot disease in patients with diabetic angiopathy.

  9. Carboxylic acids in the hindgut of rats fed highly soluble inulin and Bifidobacterium lactis (Bb-12), Lactobacillus salivarius (UCC500) or Lactobacillus rhamnosus (GG)

    PubMed Central

    Nilsson, Ulf; Nyman, Margareta

    2007-01-01

    Background Propionic and butyric acids are important nutrients for the mucosal cells and may therefore increase the nutritional status and reduce the permeability of the colonic mucosa. These acids have also been suggested to counteract diseases in the colon, e.g. ulcerative colitis and colon cancer. Different substrates lead to different amounts and patterns of carboxylic acids (CAs). Objective To study the effect of probiotics on CA formation in the hindgut of rats given inulin. Design The rats were given inulin, marketed as highly soluble by the producer, together with the probiotic bacteria Bifidobacterium lactis (Bb-12), Lactobacillus salivarius (UCC500) or Lactobacillus rhamnosus (GG), or a mixture of all three. Results Rats fed inulin only had comparatively high proportions of propionic and butyric acids throughout the hindgut. When diets were supplemented with Bb-12 and UCC500, the caecal pool of CAs increased compared with inulin only. In the caecum the proportion of butyric acid generally decreased when the rats were fed probiotics. In the distal colon the proportion of propionic and butyric acid was lower, while that of lactic acid was generally higher. The caecal pH in rats fed GG and Bb-12 was lower than expected from the concentration of CAs. Further, rats fed GG had the lowest weight gain and highest caecal tissue weight. Conclusions It is possible to modify the formation of CAs by combining inulin with probiotics. Different probiotics had different effects.

  10. Decreased susceptibility to 4'-deoxy-6'-N-methylamikacin (BB-K311) conferred by a mutant plasmid in Escherichia coli.

    PubMed

    Perlin, M H; Lerner, S A

    1982-07-01

    Escherichia coli MP6 contains a plasmid that encodes aminoglycoside 3'-phosphotransferase II, which phosphorylates kanamycin and confers high-level kanamycin resistance, Amikacin is a minor substrate of this enzyme, but MP6 is susceptible to amikacin. Strain MP10 has a spontaneous mutation in the plasmid of MP6 that increases the aminoglycoside 3'-phosphotransferase II activity not only against kanamycin but also against amikacin. This mutation is also responsible for the appearance of resistance to amikacin in MP10. Resistance to 4'-deoxy-6'-N-methylamikacin (BB-K311) by enzymatic modification has not been reported previously. As with amikacin, MP6 was susceptible to BB-K311 and its aminoglycoside 3'-phosphotransferase II did not phosphorylate this amikacin derivative appreciably. We found that the plasmid-borne mutation in MP10, however, localized by being cloned with a 3.7-megadalton HindIII fragment containing the aminoglycoside 3'-phosphotransferase II gene, resulted in increased phosphorylation of BB-K311 and resistance to it. Thus, the mutation distinguishing MP6 and MP10 has increased the activity of an existing aminoglycoside-modifying enzyme and produced new bacterial resistance to two previously minor substrates of the enzyme.

  11. Persimmon peel extract attenuates PDGF-BB-induced human aortic smooth muscle cell migration and invasion through inhibition of c-Src activity.

    PubMed

    Son, Joe Eun; Hwang, Mun Kyung; Lee, Eunjung; Seo, Sang Gwon; Kim, Jong-Eun; Jung, Sung Keun; Kim, Jong Rhan; Ahn, Gwang-Hwan; Lee, Ki Won; Lee, Hyong Joo

    2013-12-15

    The unregulated migration and invasion of human aortic smooth muscle cells (HASMCs) into the intima is a crucial step in the development of atherosclerosis. Recently, the oriental persimmon extract (Diospyros kaki Thunb. cv. Fuyu) has been investigated for its anti-atherogenic properties, but the molecular mechanisms involved remain unclear. We investigated the inhibitory effects of persimmon peel and flesh extract on the platelet-derived growth factor (PDGF) BB-induced MMP-1 expression using Western blot, and abnormal migration and invasion of HASMCs using a modified Boyden chamber assay and a wound healing assay. We also evaluated the inhibitory effects of persimmon peel extract on aortic vessel thickening using a rat aortic sprouting assay. Persimmon peel (PPE), but not flesh extract (PFE), inhibited PDGF-BB-induced MMP-1 expression, cell migration and invasion in HASMCs, while suppressing the rat aortic sprouting. Western blot and in vitro kinase assay data demonstrated that PPE inhibited Src kinase activity and subsequently attenuated PDGF-BB-induced phosphorylation of MAPK and Akt signalling pathways. Taken together, our results indicate that persimmon peel might possess a potential anti-atherogenic effect through attenuation of ASMCs migration and invasion and aortic sprouting by direct inhibition of the c-Src kinase activity.

  12. Vertical ridge augmentation using an equine block infused with recombinant human platelet-derived growth factor-BB: a histologic study in a canine model.

    PubMed

    Simion, Massimo; Nevins, Myron; Rocchietta, Isabella; Fontana, Filippo; Maschera, Emilio; Schupbach, Peter; Kim, David M

    2009-06-01

    This preclinical study evaluated the efficacy of purified recombinant human platelet-derived growth factor (rhPDGF-BB), combined with a novel equine hydroxyapatite and collagen (eHAC) bone block, in providing vertical bone regeneration in critical-size defects simulating localized mandibular alveolar bone atrophy. In addition, the impact of barrier membrane placement in growth factor-mediated bone regeneration was also studied. Bilateral posterior mandibular defects simulating severe localized bony atrophy were created in 12 adult foxhounds following removal of all four mandibular premolars. Three months later, the defects were grafted as follows: group A: eHAC block alone; group B: eHAC block + collagen membrane; group C: eHAC block + rhPDGF-BB; group D: eHAC block + rhPDGF-BB + membrane. The animals were sacrificed after 5 months and the grafted areas were examined histologically, radiographically, and clinically. Groups A and B (controls) exhibited little to no vertical bone regeneration. Group C demonstrated significant vertical bone regeneration, with dense, well-vascularized bone, high bone-to-implant contact, and accelerated replacement of graft particles with newly formed bone. In group D, with the imposition of a barrier membrane, robust bone regeneration was less evident when compared to group C. As in the first study in this series, the importance of the periosteum as a source of osteoprogenitor cells in growth factor-mediated regenerative procedures is examined.

  13. Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 cell surface hydrophobicity and survival of the cells under adverse environmental conditions.

    PubMed

    Shakirova, Laisana; Grube, Mara; Gavare, Marita; Auzina, Lilija; Zikmanis, Peteris

    2013-01-01

    Changes in the cell surface hydrophobicity (CSH) of probiotic bacteria Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 and the survival of these cells were examined in response to varied cultivation conditions and adverse environmental conditions. An inverse linear relationship (P < 0.01) was detected between the CSH of intact L. acidophilus La5 and B. lactis Bb12 and survival of cells subjected to subsequent freezing/thawing, long-term storage or exposure to mineral and bile acids. The observed relationships were supported by significant correlations between the CSH and changes in composition of the cell envelopes (proteins, lipids and carbohydrates) of L. acidophilus La5 and B. lactis Bb12 examined using FT-IR spectroscopy and conventional biochemical analysis methods. The results also suggest that the estimates of hydrophobicity, being a generalized characteristic of cell surfaces, are important parameters to predict the ability of intact probiotic bacteria to endure extreme environments and therefore should be monitored during cultivation. A defined balance of cell components, which can be characterized by the reduced CSH values, apparently helps to ensure the resistance, improved viability and hence the overall probiotic properties of bacteria.

  14. Knockdown of fbxl10/kdm2bb rescues chd7 morphant phenotype in a zebrafish model of CHARGE syndrome

    PubMed Central

    Balow, Stephanie A.; Pierce, Lain X.; Zentner, Gabriel E.; Conrad, Patricia A.; Davis, Stephani; Sabaawy, Hatem E.; McDermott, Brian M.; Scacheri, Peter C.

    2013-01-01

    CHARGE syndrome is a sporadic autosomal-dominant genetic disorder characterized by a complex array of birth defects so named for its cardinal features of ocular coloboma, heart defects, choanal atresia, growth retardation, genital abnormalities, and ear abnormalities. Approximately two-thirds of individuals clinically diagnosed with CHARGE syndrome have heterozygous loss-of-function mutations in the gene encoding chromodomain helicase DNA-binding protein 7 (CHD7), an ATP-dependent chromatin remodeler. To examine the role of Chd7 in development, a zebrafish model was generated through morpholino (MO)-mediated targeting of the zebrafish chd7 transcript. High doses of chd7 MO induce lethality early in embryonic development. However, low dose-injected embryos are viable, and by 4 days post-fertilization, morphant fish display multiple defects in organ systems analogous to those affected in humans with CHARGE syndrome. The chd7 morphants show elevated expression of several potent cell-cycle inhibitors including ink4ab (p16/p15), p21 and p27, accompanied by reduced cell proliferation. We also show that Chd7 is required for proper organization of neural crest-derived craniofacial cartilage structures. Strikingly, MO-mediated knockdown of the jumonji domain-containing histone demethylase fbxl10/kdm2bb, a repressor of ribosomal RNA (rRNA) genes, rescues cell proliferation and cartilage defects in chd7 morphant embryos and can lead to complete rescue of the CHARGE syndrome phenotype. These results indicate that CHARGE-like phenotypes in zebrafish can be mitigated through modulation of fbxl10 levels and implicate FBXL10 as a possible therapeutic target in CHARGE syndrome. PMID:23920116

  15. Impact of the Bt corn proteins Cry34/35Ab1 and Cry3Bb1, alone or pyramided, on western corn rootworm (Coleoptera: Chrysomelidae) beetle emergence in the field

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a major pest of corn, Zea mays L. This study compared the effect of the Bt proteins Cry34/35Ab1, Cry3Bb1, singly expressed, and Cry3Bb1 plus Cry34/35Ab1 in a pyramid, with a near-isoline control, on D. virgifera adult emergence in fie...

  16. Search for bb¯ decay of the Stand Model Higgs boson produced in association with a vector boson (W/Z) with the ATLAS detector

    NASA Astrophysics Data System (ADS)

    Ming, Yao

    This dissertation presents a search for the bb¯ decay of the Standard Model Higgs boson. The analysis is performed with the ATLAS experiment, using the full dataset delivered by the LHC and recorded by ATLAS detector during LHC Run 1. The integrated luminosities used are 4.7 fb-1 at √s = 7 TeV, and 20.3 fb-1 at √s = 8 TeV. The processes considered in this analysis are associated (W/Z)H production, where W → ℓnunu, Z → ℓℓ and Z → nunu. Based on the number of leptons (ℓ), the events used in this analysis are divided into zero, one, and two lepton channels. In this analysis, no significant excess is observed above the Standard Model backgrounds. For mH = 125 GeV, a 95% CL upper limit of 1.4 times the Standard Model expectation is set on the cross section times branching ratio for pp → (W/Z )(H → bb¯). The corresponding expected limit is 1.3 in the absence of signal. The ratio of the measured signal yield to the Standard Model expectation is found to be mu = 0.2 +/- 0.5(stat.) +/- 0.4(syst.). This analysis procedure is validated by a measurement of the yield of diboson production(WZ and ZZ), with Z → bb¯. The ratio of observed diboson signal strength to the Standard Model expectation is found to be muD = 0.93 +/- 0.21, which is consistent with the Standard Model expectation of muD = 1.

  17. Assessment of prey-mediated effects of the coleopteran-specific toxin Cry3Bb1 on the generalist predator Atheta coriaria (Coleoptera: Staphylinidae).

    PubMed

    García, M; Ortego, F; Castañera, P; Farinós, G P

    2012-06-01

    A laboratory study was carried out to assess the potential prey-mediated effects of Cry3Bb1-expressing Bt maize on the fitness and predatory ability of Atheta coriaria Kraatz (Coleoptera: Staphylinidae), using Tetranychus urticae Koch (Acari: Tetranychidae) as prey. The concentration of Cry3Bb1 toxin through the trophic chain significantly decreased from Bt maize (21.7 μg g(-1) FW) to mites (5.6 μg g(-1) FW) and then to A. coriaria adults (1.4 μg g(-1) FW), but not from mites to A. coriaria L1-L3 larvae (4.1-4.6 μg g(-1) FW). Interestingly, the toxin levels detected in A. coriaria larvae represent more than 20% of the concentration found in Bt maize, and the toxin was detected up to 48 h after exposure. To our knowledge, this is the highest level of exposure ever reported in a predatory beetle to the Cry3Bb1 protein. When A. coriaria larvae were reared on Bt-fed mites, Bt-free mites or rearing food, no significant differences among treatments were observed in development, morphological measurements of sclerotized structures and body weight. Moreover, no negative effects on reproductive parameters were reported in adults feeding on Bt-fed prey after 30 days of treatment, and survival was not affected after 60 days of exposure. Similarly, predatory ability and prey consumption of A. coriaria larvae and adults were not affected by exposure to the toxin. All together, these results indicate a lack of adverse effects on A. coriaria, a species commonly used as a biological control agent. The use of A. coriaria as a surrogate species for risk assessment of GM crops that express insecticidal proteins is discussed.

  18. Combined Transcriptome and Proteome Analysis of Bifidobacterium animalis subsp. lactis BB-12 Grown on Xylo-Oligosaccharides and a Model of Their Utilization▿ †

    PubMed Central

    Gilad, Ofir; Jacobsen, Susanne; Stuer-Lauridsen, Birgitte; Pedersen, Martin Bastian; Garrigues, Christel; Svensson, Birte

    2010-01-01

    Recent studies have demonstrated that xylo-oligosaccharides (XOS), which are classified as emerging prebiotics, selectively enhance the growth of bifidobacteria in general and of Bifidobacterium animalis subsp. lactis strains in particular. To elucidate the metabolism of XOS in the well-documented and widely used probiotic strain B. animalis subsp. lactis BB-12, a combined proteomic and transcriptomic approach was applied, involving DNA microarrays, real-time quantitative PCR (qPCR), and two-dimensional difference gel electrophoresis (2D-DIGE) analyses of samples obtained from cultures grown on either XOS or glucose. The analyses show that 9 of the 10 genes that encode proteins predicted to play a role in XOS catabolism (i.e., XOS-degrading and -metabolizing enzymes, transport proteins, and a regulatory protein) were induced by XOS at the transcriptional level, and the proteins encoded by three of these (β-d-xylosidase, sugar-binding protein, and xylose isomerase) showed higher abundance on XOS. Based on the obtained results, a model for the catabolism of XOS in BB-12 is suggested, according to which the strain utilizes an ABC (ATP-binding cassette) transport system (probably for oligosaccharides) to bind XOS on the cell surface and transport them into the cell. XOS are then degraded intracellularly through the action of xylanases and xylosidases to d-xylose, which is subsequently metabolized by the d-fructose-6-P shunt. The findings obtained in this study may have implications for the design of a synbiotic application containing BB-12 and the XOS used in the present study. PMID:20851982

  19. Effect of Lactobacillus rhamnosus LGG® and Bifidobacterium animalis ssp. lactis BB-12® on health-related quality of life in college students affected by upper respiratory infections.

    PubMed

    Smith, Tracey J; Rigassio-Radler, Diane; Denmark, Robert; Haley, Timothy; Touger-Decker, Riva

    2013-06-01

    College students are susceptible to upper respiratory infections (URI) due to inadequate sleep, stress and close living quarters. Certain probiotic strains modulate immune function and may improve health-related quality of life (HRQL) during URI. The present study recruited apparently healthy college students and assessed the effect of probiotics on HRQL outcomes (i.e. self-reported duration, symptom severity and functional impairment of URI) in those who developed URI. Missed school and work days due to URI were also considered. Subjects (n 231) were apparently healthy college students living on campus in residence halls at the Framingham State University (Framingham, MA, USA), and were randomised to receive placebo (n 117) or probiotic-containing powder (daily dose of minimum 1 billion colony-forming units of each Lactobacillus rhamnosus LGG® (LGG®) and Bifidobacterium animalis ssp. lactis BB-12® (BB-12®); n 114) for 12 weeks. Subjects completed The Wisconsin Upper Respiratory Symptom Survey-21 to assess HRQL during URI. The final analyses included 198 subjects (placebo, n 97 and probiotics, n 101). The median duration of URI was significantly shorter by 2 d and median severity score was significantly lower by 34% with probiotics v. placebo (P,0·001), indicating a higher HRQL during URI. Number of missed work days was not different between groups (P=0·429); however, the probiotics group missed significantly fewer school days (mean difference = 0·2 d) compared to the placebo group (P=0·002). LGG® and BB-12® may be beneficial among college students with URI for mitigating decrements in HRQL. More research is warranted regarding mechanisms of action associated with these findings and the cost-benefit of prophylactic supplementation.

  20. Search for resonant pair production of neutral long-lived particles decaying to bb in pp collisions at square root(S)=1.96 TeV.

    PubMed

    Abazov, V M; Abbott, B; Abolins, M; Acharya, B S; Adams, M; Adams, T; Aguilo, E; Ahsan, M; Alexeev, G D; Alkhazov, G; Alton, A; Alverson, G; Alves, G A; Ancu, L S; Andeen, T; Anzelc, M S; Aoki, M; Arnoud, Y; Arov, M; Arthaud, M; Askew, A; Asman, B; Atramentov, O; Avila, C; Backusmayes, J; Badaud, F; Bagby, L; Baldin, B; Bandurin, D V; Banerjee, S; Barberis, E; Barfuss, A-F; Bargassa, P; Baringer, P; Barreto, J; Bartlett, J F; Bassler, U; Bauer, D; Beale, S; Bean, A; Begalli, M; Begel, M; Belanger-Champagne, C; Bellantoni, L; Bellavance, A; Benitez, J A; Beri, S B; Bernardi, G; Bernhard, R; Bertram, I; Besançon, M; Beuselinck, R; Bezzubov, V A; Bhat, P C; Bhatnagar, V; Blazey, G; Blessing, S; Bloom, K; Boehnlein, A; Boline, D; Bolton, T A; Boos, E E; Borissov, G; Bose, T; Brandt, A; Brock, R; Brooijmans, G; Bross, A; Brown, D; Bu, X B; Buchholz, D; Buehler, M; Buescher, V; Bunichev, V; Burdin, S; Burnett, T H; Buszello, C P; Calfayan, P; Calpas, B; Calvet, S; Cammin, J; Carrasco-Lizarraga, M A; Carrera, E; Carvalho, W; Casey, B C K; Castilla-Valdez, H; Chakrabarti, S; Chakraborty, D; Chan, K M; Chandra, A; Cheu, E; Cho, D K; Choi, S; Choudhary, B; Christoudias, T; Cihangir, S; Claes, D; Clutter, J; Cooke, M; Cooper, W E; Corcoran, M; Couderc, F; Cousinou, M-C; Crépé-Renaudin, S; Cutts, D; Cwiok, M; Das, A; Davies, G; De, K; de Jong, S J; De La Cruz-Burelo, E; Devaughan, K; Déliot, F; Demarteau, M; Demina, R; Denisov, D; Denisov, S P; Desai, S; Diehl, H T; Diesburg, M; Dominguez, A; Dorland, T; Dubey, A; Dudko, L V; Duflot, L; Duggan, D; Duperrin, A; Dutt, S; Dyshkant, A; Eads, M; Edmunds, D; Ellison, J; Elvira, V D; Enari, Y; Eno, S; Escalier, M; Evans, H; Evdokimov, A; Evdokimov, V N; Facini, G; Ferapontov, A V; Ferbel, T; Fiedler, F; Filthaut, F; Fisher, W; Fisk, H E; Fortner, M; Fox, H; Fu, S; Fuess, S; Gadfort, T; Galea, C F; Garcia-Bellido, A; Gavrilov, V; Gay, P; Geist, W; Geng, W; Gerber, C E; Gershtein, Y; Gillberg, D; Ginther, G; Gómez, B; Goussiou, A; Grannis, P D; Greder, S; Greenlee, H; Greenwood, Z D; Gregores, E M; Grenier, G; Gris, Ph; Grivaz, J-F; Grohsjean, A; Grünendahl, S; Grünewald, M W; Guo, F; Guo, J; Gutierrez, G; Gutierrez, P; Haas, A; Haefner, P; Hagopian, S; Haley, J; Hall, I; Hall, R E; Han, L; Harder, K; Harel, A; Hauptman, J M; Hays, J; Hebbeker, T; Hedin, D; Hegeman, J G; Heinson, A P; Heintz, U; Hensel, C; Heredia-De La Cruz, I; Herner, K; Hesketh, G; Hildreth, M D; Hirosky, R; Hoang, T; Hobbs, J D; Hoeneisen, B; Hohlfeld, M; Hossain, S; Houben, P; Hu, Y; Hubacek, Z; Huske, N; Hynek, V; Iashvili, I; Illingworth, R; Ito, A S; Jabeen, S; Jaffré, M; Jain, S; Jakobs, K; Jamin, D; Jesik, R; Johns, K; Johnson, C; Johnson, M; Johnston, D; Jonckheere, A; Jonsson, P; Juste, A; Kajfasz, E; Karmanov, D; Kasper, P A; Katsanos, I; Kaushik, V; Kehoe, R; Kermiche, S; Khalatyan, N; Khanov, A; Kharchilava, A; Kharzheev, Y N; Khatidze, D; Kim, T J; Kirby, M H; Kirsch, M; Klima, B; Kohli, J M; Konrath, J-P; Kozelov, A V; Kraus, J; Kuhl, T; Kumar, A; Kupco, A; Kurca, T; Kuzmin, V A; Kvita, J; Lacroix, F; Lam, D; Lammers, S; Landsberg, G; Lebrun, P; Lee, W M; Leflat, A; Lellouch, J; Li, J; Li, L; Li, Q Z; Lietti, S M; Lim, J K; Lincoln, D; Linnemann, J; Lipaev, V V; Lipton, R; Liu, Y; Liu, Z; Lobodenko, A; Lokajicek, M; Love, P; Lubatti, H J; Luna-Garcia, R; Lyon, A L; Maciel, A K A; Mackin, D; Mättig, P; Magaña-Villalba, R; Magerkurth, A; Mal, P K; Malbouisson, H B; Malik, S; Malyshev, V L; Maravin, Y; Martin, B; McCarthy, R; McGivern, C L; Meijer, M M; Melnitchouk, A; Mendoza, L; Menezes, D; Mercadante, P G; Merkin, M; Merritt, K W; Meyer, A; Meyer, J; Mitrevski, J; Mondal, N K; Moore, R W; Moulik, T; Muanza, G S; Mulhearn, M; Mundal, O; Mundim, L; Nagy, E; Naimuddin, M; Narain, M; Neal, H A; Negret, J P; Neustroev, P; Nilsen, H; Nogima, H; Novaes, S F; Nunnemann, T; Obrant, G; Ochando, C; Onoprienko, D; Orduna, J; Oshima, N; Osman, N; Osta, J; Otec, R; Otero Y Garzón, G J; Owen, M; Padilla, M; Padley, P; Pangilinan, M; Parashar, N; Park, S-J; Park, S K; Parsons, J; Partridge, R; Parua, N; Patwa, A; Pawloski, G; Penning, B; Perfilov, M; Peters, K; Peters, Y; Pétroff, P; Piegaia, R; Piper, J; Pleier, M-A; Podesta-Lerma, P L M; Podstavkov, V M; Pogorelov, Y; Pol, M-E; Polozov, P; Popov, A V; da Silva, W L Prado; Protopopescu, S; Qian, J; Quadt, A; Quinn, B; Rakitine, A; Rangel, M S; Ranjan, K; Ratoff, P N; Renkel, P; Rich, P; Rijssenbeek, M; Ripp-Baudot, I; Rizatdinova, F; Robinson, S; Rominsky, M; Royon, C; Rubinov, P; Ruchti, R; Safronov, G; Sajot, G; Sánchez-Hernández, A; Sanders, M P; Sanghi, B; Savage, G; Sawyer, L; Scanlon, T; Schaile, D; Schamberger, R D; Scheglov, Y; Schellman, H; Schliephake, T; Schlobohm, S; Schwanenberger, C; Schwienhorst, R; Sekaric, J; Severini, H; Shabalina, E; Shamim, M; Shary, V; Shchukin, A A; Shivpuri, R K; Siccardi, V; Simak, V; Sirotenko, V; Skubic, P; Slattery, P; Smirnov, D; Snow, G R; Snow, J; Snyder, S; Söldner-Rembold, S; Sonnenschein, L; Sopczak, A; Sosebee, M; Soustruznik, K; Spurlock, B; Stark, J; Stolin, V; Stoyanova, D A; Strandberg, J; Strang, M A; Strauss, E; Strauss, M; Ströhmer, R; Strom, D; Stutte, L; Sumowidagdo, S; Svoisky, P; Takahashi, M; Tanasijczuk, A; Taylor, W; Tiller, B; Titov, M; Tokmenin, V V; Torchiani, I; Tsybychev, D; Tuchming, B; Tully, C; Tuts, P M; Unalan, R; Uvarov, L; Uvarov, S; Uzunyan, S; van den Berg, P J; Van Kooten, R; van Leeuwen, W M; Varelas, N; Varnes, E W; Vasilyev, I A; Verdier, P; Vertogradov, L S; Verzocchi, M; Vilanova, D; Vint, P; Vokac, P; Voutilainen, M; Wagner, R; Wahl, H D; Wang, M H L S; Warchol, J; Watts, G; Wayne, M; Weber, G; Weber, M; Welty-Rieger, L; Wenger, A; Wetstein, M; White, A; Wicke, D; Williams, M R J; Wilson, G W; Wimpenny, S J; Wobisch, M; Wood, D R; Wyatt, T R; Xie, Y; Xu, C; Yacoob, S; Yamada, R; Yang, W-C; Yasuda, T; Yatsunenko, Y A; Ye, Z; Yin, H; Yip, K; Yoo, H D; Youn, S W; Yu, J; Zeitnitz, C; Zelitch, S; Zhao, T; Zhou, B; Zhu, J; Zielinski, M; Zieminska, D; Zivkovic, L; Zutshi, V; Zverev, E G

    2009-08-14

    We report on a first search for resonant pair production of neutral long-lived particles (NLLP) which each decay to a bb pair, using 3.6 fb(-1) of data recorded with the D0 detector at the Fermilab Tevatron collider. We search for pairs of displaced vertices in the tracking detector at radii in the range 1.6-20 cm from the beam axis. No significant excess is observed above background, and upper limits are set on the production rate in a hidden-valley benchmark model for a range of Higgs boson masses and NLLP masses and lifetimes.

  1. Measurement of the e;{+}e;{-}-->bb[over ] Cross Section between sqrt[s]=10.54 and 11.20 GeV.

    PubMed

    Aubert, B; Bona, M; Karyotakis, Y; Lees, J P; Poireau, V; Prencipe, E; Prudent, X; Tisserand, V; Garra Tico, J; Grauges, E; Lopez, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Cahn, R N; Jacobsen, R G; Kerth, L T; Kolomensky, Yu G; Lynch, G; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabe, T; Hawkes, C M; Soni, N; Watson, A T; Koch, H; Schroeder, T; Walker, D; Asgeirsson, D J; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Sharma, V; Campagnari, C; Hong, T M; Kovalskyi, D; Mazur, M A; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Martinez, A J; Schalk, T; Schumm, B A; Seiden, A; Wilson, M G; Winstrom, L O; Cheng, C H; Doll, D A; Echenard, B; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Bloom, P C; Ford, W T; Gaz, A; Hirschauer, J F; Nagel, M; Nauenberg, U; Smith, J G; Ulmer, K A; Wagner, S R; Ayad, R; Soffer, A; Toki, W H; Wilson, R J; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Karbach, M; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Kobel, M J; Mader, W F; Nogowski, R; Schubert, K R; Schwierz, R; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Verderi, M; Clark, P J; Playfer, S; Watson, J E; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Santoro, V; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Adametz, A; Marks, J; Schenk, S; Uwer, U; Klose, V; Lacker, H M; Bard, D J; Dauncey, P D; Nash, J A; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Cochran, J; Crawley, H B; Dong, L; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Arnaud, N; Béquilleux, J; D'Orazio, A; Davier, M; Firmino da Costa, J; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Touramanis, C; Bevan, A J; Clarke, C K; George, K A; Di Lodovico, F; Sacco, R; Sigamani, M; Cowan, G; Flaecher, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Denig, A G; Fritsch, M; Gradl, W; Schott, G; Alwyn, K E; Bailey, D; Barlow, R J; Chia, Y M; Edgar, C L; Jackson, G; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Dallapiccola, C; Li, X; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Sciolla, G; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, G; Lista, L; Monorchio, D; Onorato, G; Sciacca, C; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; Losecco, J M; Wang, W F; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Sekula, S J; Wong, Q K; Blount, N L; Brau, J; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Castelli, G; Gagliardi, N; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Del Amo Sanchez, P; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; Hamon, O; Leruste, Ph; Ocariz, J; Perez, A; Prendki, J; Sitt, S; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Carpinelli, M; Cervelli, A; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J J; Lopes Pegna, D; Lu, C; Olsen, J; Smith, A J S; Telnov, A V; Anulli, F; Baracchini, E; Cavoto, G; Del Re, D; Di Marco, E; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Jackson, P D; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Renga, F; Voena, C; Ebert, M; Hartmann, T; Schröder, H; Waldi, R; Adye, T; Franek, B; Olaiya, E O; Wilson, F F; Emery, S; Escalier, M; Esteve, L; Ganzhur, S F; Hamel de Monchenault, G; Kozanecki, W; Vasseur, G; Yèche, Ch; Zito, M; Chen, X R; Liu, H; Park, W; Purohit, M V; White, R M; Wilson, J R; Allen, M T; Aston, D; Bartoldus, R; Bechtle, P; Benitez, J F; Bertsche, K; Cai, Y; Cenci, R; Coleman, J P; Convery, M R; Decker, F J; Dingfelder, J C; Dorfan, J; Dubois-Felsmann, G P; Dunwoodie, W; Ecklund, S; Erickson, R; Field, R C; Fisher, A; Fox, J; Gabareen, A M; Gowdy, S J; Graham, M T; Grenier, P; Hast, C; Innes, W R; Iverson, R; Kaminski, J; Kelsey, M H; Kim, H; Kim, P; Kocian, M L; Kulikov, A; Leith, D W G S; Li, S; Lindquist, B; Luitz, S; Luth, V; Lynch, H L; Macfarlane, D B; Marsiske, H; Messner, R; Muller, D R; Neal, H; Nelson, S; Novokhatski, A; O'Grady, C P; Ofte, I; Perazzo, A; Perl, M; Ratcliff, B N; Rivetta, C; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Seeman, J; Snyder, A; Su, D; Sullivan, M K; Suzuki, K; Swain, S K; Thompson, J M; Va'vra, J; Van Winkle, D; Wagner, A P; Weaver, M; West, C A; Wienands, U; Wisniewski, W J; Wittgen, M; Wittmer, W; Wright, D H; Wulsin, H W; Yan, Y; Yarritu, A K; Yi, K; Yocky, G; Young, C C; Ziegler, V; Burchat, P R; Edwards, A J; Majewski, S A; Miyashita, T S; Petersen, B A; Wilden, L; Ahmed, S; Alam, M S; Ernst, J A; Pan, B; Saeed, M A; Zain, S B; Spanier, S M; Wogsland, B J; Eckmann, R; Ritchie, J L; Ruland, A M; Schilling, C J; Schwitters, R F; Drummond, B W; Izen, J M; Lou, X C; Bianchi, F; Gamba, D; Pelliccioni, M; Bomben, M; Bosisio, L; Cartaro, C; Della Ricca, G; Lanceri, L; Vitale, L; Azzolini, V; Lopez-March, N; Martinez-Vidal, F; Milanes, D A; Oyanguren, A; Albert, J; Banerjee, Sw; Bhuyan, B; Choi, H H F; Hamano, K; Kowalewski, R; Lewczuk, M J; Nugent, I M; Roney, J M; Sobie, R J; Gershon, T J; Harrison, P F; Ilic, J; Latham, T E; Mohanty, G B; Band, H R; Chen, X; Dasu, S; Flood, K T; Pan, Y; Pierini, M; Prepost, R; Vuosalo, C O; Wu, S L

    2009-01-09

    We report e;{+}e;{-}-->bb[over ] cross section measurements by the BABAR experiment performed during an energy scan in the range of 10.54 to 11.20 GeV at the SLAC PEP-II e;{+}e;{-} collider. A total relative error of about 5% is reached in more than 300 center-of-mass energy steps, separated by about 5 MeV. These measurements can be used to derive precise information on the parameters of the Upsilon(10860) and Upsilon(11020) resonances. In particular we show that their widths may be smaller than previously measured.

  2. Impact of wind on ambient noise recorded by the "13 BB star" seismic array in northern Poland

    NASA Astrophysics Data System (ADS)

    Lepore, Simone; Markowicz, Krzysztof; Grad, Marek

    2016-04-01

    Seismic interferometry and beam forming techniques were applied to ambient noise recorded during January 2014 at the "13 BB star" array, composed of thirteen seismic stations located in northern Poland, with the aim of evaluating the azimuth of noise sources and the velocities of surface waves. After normalizing the raw recordings in time and frequency domain, the spectral characteristics of the ambient noise were studied to choose a frequency band suitable for the waves' retrieval. To get the velocity of surface waves by seismic interferometry, the crosscorrelation between all station pairs was analysed for the vertical and horizontal components in the 0.05-0.1 Hz, 0.1-1 Hz and 1 10 Hz frequency bands. For each pair, the crosscorrelation was applied to one hour recordings extracted from the ambient noise. The obtained traces were calculated for a complete day, and then summed together: the daily results were stacked for the whole January 2014. In the lowest frequency range, most of the energy is located around the 3.0 km/s line, meaning that the surface waves coming from the uppermost mantle will be retrieved. The intermediate frequency range shows most of the energy between the 2.0 km/s and 1.5 km/s lines: consequently, surface waves originating from the crust will be retrieved. In the highest frequency range, the surface waves are barely visible on the crosscorrelation traces, implying that the associated energy is strongly attenuated. The azimuth variation associated to the noise field was evaluated by means of the beam forming method, using the data from the whole array for all the three components. To that, the beam power was estimated in a small range of frequencies every day for the whole month. For each day, one hour long results of beam forming applications were stacked together. To avoid aliasing and near field effects, the minimum frequency was set at 0.05 Hz and the maximum to 0.1 Hz. In this frequency band, the amplitude maximum was sought

  3. Guanine nucleotide binding regulatory proteins and adenylate cyclase in livers of streptozotocin- and BB/Wor-diabetic rats. Immunodetection of Gs and Gi with antisera prepared against synthetic peptides.

    PubMed Central

    Lynch, C J; Blackmore, P F; Johnson, E H; Wange, R L; Krone, P K; Exton, J H

    1989-01-01

    Adenylate cyclase in liver plasma membranes from streptozotocin-diabetic (STZ) or BB/Wor spontaneously diabetic rats showed increased responsiveness to GTP, glucagon, fluoroaluminate, and cholera toxin. Basal or forskolin-stimulated activity was unchanged in STZ rats, but increased in BB/Wor rats. No change in the alpha-subunit of Gi (alpha i) was observed in STZ or BB/Wor rats using pertussis toxin-stimulated [32P]ADP-ribosylation. Immunodetection using antibodies against the COOH-terminal decapeptides of alpha T and alpha i-3 showed no change in alpha i in STZ rats and a slight decrease in BB/Wor rats. Angiotensin II inhibition of hepatic adenylate cyclase was not altered in either diabetic rat. In both models of diabetes, Gs alpha-subunits were increased as measured by cholera toxin-stimulated [32P]-ADP-ribosylation of 43-47.5-kD peptides, reconstitution with membranes from S49 cyc- cells or immunoreactivity using antibodies against the COOH-terminal decapeptide of alpha s. These data indicate that STZ-diabetes increases hepatic Gs but does not change Gi or adenylate cyclase catalytic activity. In contrast, BB/Wor rats show increased hepatic Gs and adenylate cyclase. These changes could explain the increase in hepatic cAMP and related dysfunctions observed in diabetes. Images PMID:2498395

  4. Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma.

    PubMed

    Neri, Shinya; Miyashita, Tomoyuki; Hashimoto, Hiroko; Suda, Yoshitaka; Ishibashi, Masayuki; Kii, Hiroaki; Watanabe, Hirotada; Kuwata, Takeshi; Tsuboi, Masahiro; Goto, Koichi; Menju, Toshi; Sonobe, Makoto; Date, Hiroshi; Ochiai, Atsushi; Ishii, Genichiro

    2017-06-01

    Cancer-associated fibroblast (CAF)-dependent local invasion is the process by which cancer cells invade the extracellular matrix using tracks that have been physically remodeled by CAFs. In the present study, we investigated the process by which the epithelial-mesenchymal transition (EMT) of cancer cells affect CAF-dependent local invasion. Using an in vitro collagen invasion assay, we showed cancer cells undergoing EMT to promote the matrix-remodeling ability of CAFs and thereby enhance CAF-dependent local cancer cell invasion. Platelet-derived growth factor (PDGF)-BB secretion was significantly elevated in cancer cells undergoing EMT, and this induced an increase in the invasion ability of both CAFs and cancer cells. Conversely, knockdown of PDGF-B expression in cancer cells undergoing EMT, or treatment with a PDGF-receptor inhibitor, decreased the invasion ability of both CAFs and cancer cells. By analyzing the gene expression profiles of 442 patients with lung adenocarcinomas, we established that high expression of PDGF-B and presentation of mesenchymal-like tumors were significantly associated with a high rate of disease recurrence and poor patient prognosis. Thus, cancer cells undergoing EMT may accelerate their own ability to invade local tissues via PDGF-BB secretion to promote CAF matrix remodeling. Therefore, targeting PDGF signaling between cancer cells undergoing EMT and CAFs is a promising therapeutic target to inhibit cancer progression and improve patient prognosis.

  5. Effect of yoghurt containing Bifidobacterium lactis Bb12® on faecal excretion of secretory immunoglobulin A and human beta-defensin 2 in healthy adult volunteers

    PubMed Central

    2011-01-01

    Background Probiotics are used to provide health benefits. The present study tested the effect of a probiotic yoghurt on faecal output of beta-defensin and immunoglobulin A in a group of young healthy women eating a defined diet. Findings 26 women aged 18-21 (median 19) years residing in a hostel were given 200 ml normal yoghurt every day for a week, followed by probiotic yoghurt containing Bifidobacterium lactis Bb12® (109 in 200 ml) for three weeks, followed again by normal yoghurt for four weeks. Stool samples were collected at 0, 4 and 8 weeks and assayed for immunoglobulin A and human beta-defensin-2 by ELISA. All participants tolerated both normal and probiotic yoghurt well. Human beta-defensin-2 levels in faeces were not altered during the course of the study. On the other hand, compared to the basal sample, faecal IgA increased during probiotic feeding (P = 0.0184) and returned to normal after cessation of probiotic yoghurt intake. Conclusions Bifidobacterium lactis Bb12® increased secretory IgA output in faeces. This property may explain the ability of probiotics to prevent gastrointestinal and lower respiratory tract infections. PMID:22196482

  6. Synergistic effect of deoxyspergualin (DSP) and cyclosporin A (CsA) in the prevention of spontaneous autoimmune diabetes in BB rats

    PubMed Central

    DI MARCO, R; ZACCONE, P; MAGRO, G; GRASSO, S; LUNETTA, M; BARCELLINI, W; NICOLOSI, V M; MERONI, P L; NICOLETTI, F

    1996-01-01

    Dose-dependent side effects are frequently observed with immunosuppressive drugs of potential relevance for the immunotherapy of insulin-dependent diabetes mellitus (IDDM), such as CsA and DSP. If CsA and DSP acted synergistically in vivo, their combined use would allow using each compound at lower doses than those required when each drug is given in monotherapy. Consequently, dose-dependent side effects could be reduced and the therapeutic activity maintained or even enforced. Toward this end we studied the effects of combined treatment with CsA and DSP on the course of IDDM in the diabetes-prone (DP)-BB rat. The results show that two ‘low’ doses of CsA (2mg/kg) and DSP (1mg/kg) that are clinically ineffective in suppressing IDDM development in BB rats when administered alone under a prolonged prophylactic regimen (30–105 days old), may successfully prevent, but not cure, the disease when given contemporaneously under the same experimental conditions. The combined treatment was well tolerated, and no side effects were noticed. These data suggest that the combined use of CsA and DSP may deserve consideration for its possible application in the prevention/treatment of human IDDM and other autoimmune diseases. PMID:8706343

  7. Characterisation of the Poly-(Vinylpyrrolidone)-Poly-(Vinylacetate-Co-Crotonic Acid) (PVP:PVAc-CA) Interpolymer Complex Matrix Microparticles Encapsulating a Bifidobacterium lactis Bb12 Probiotic Strain.

    PubMed

    Mamvura, C I; Moolman, F S; Kalombo, L; Hall, A N; Thantsha, M S

    2011-06-01

    The method of producing poly-(vinylpyrrolidone)-poly-(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex matrix microparticles in supercritical carbon dioxide (scCO2), encapsulating bacteria, has recently been developed. This study was aimed at probing the external and internal structure of these microparticles, which can be used in food. The encapsulation efficiency and distribution of encapsulated Bifidobacterium lactis Bb12 within these microparticles were also investigated. Scanning electron microscopy (SEM) revealed irregular, mostly small, smooth microparticles with no visible bacterial cells on the surface. However, some of the microparticles appeared to have porous surfaces. The results of a Microtrac S3500 particle size analyzer showed that the PVP:PVAc-CA interpolymer complex matrix microparticles encapsulating B. lactis Bb12 had an average particle size of 166.1 μm (<350 μm designated standard size for microparticles). The D 10, D 50 and D 90 values for these microparticles were 48.16, 166.06 and 382.55 μm, respectively. Both SEM and confocal laser scanning microscopy showed a high density of bacterial cells within the microparticles. An average encapsulation efficiency of 96% was achieved. Consequently, the microparticles have the potential to be evenly distributed in foods, deliver adequate amounts of probiotics and produce minimal adverse effects on the texture and mouth feel of the foods into which they are incorporated.

  8. In vitro interaction of Metarhizium anisopliae Ma9236 and Beauveria bassiana Bb9205 with Heterorhabditis bacteriophora HNI0100 for the control of Plutella xylostella.

    PubMed

    Correa-Cuadros, J P; Sáenz-Aponte, A; Rodríguez-Bocanegra, M X

    2016-01-01

    The diamondback moth (Plutella xylostella) is a major pest of broccoli crops in Colombia. To control P. xylostella, we evaluated the interaction of Beauveria bassiana Bb9205 and Metarhizium anisopliae Ma9236 with Heterorhabditis bacteriophora HNI0100 and its bacterial symbiont Photorhabdus luminescens HNI0100. We used antagonism and disk diffusion assays with fungal extracts to test the interaction between symbiotic bacterium and fungi. P. luminescens inhibited the growth of B. bassiana and M. anisopliae up to 40% by the secretion of secondary metabolites, whereas fungal extracts did not inhibit P. luminescens; this explains the in vivo interactions of these biological control agents. To test the interaction between fungi and nematodes, we first inoculated the fungi followed by the nematodes on different days (0, 2, 4, and 6). We identified the type of interaction using the formula by Nishimatsu and Jackson (J Econ Entomol 91:410-418, 1998) and established that on days 0, 2 and 4 there was an antagonistic interaction, while a synergistic interaction occurred on day 6. Therefore, the use of the interaction between H. bacteriophora HNI0100 with M. anisopliae Ma9236 and B. bassiana Bb9205 is an innovative alternative for the control of P. xylostella.

  9. Risk assessment of Soulatrolide and Mammea (A/BA+A/BB) coumarins from Calophyllum brasiliense by a toxicogenomic and toxicological approach.

    PubMed

    Gomez-Verjan, J C; Estrella-Parra, E; Vazquez-Martinez, E R; Gonzalez-Sanchez, I; Guerrero-Magos, G; Mendoza-Villanueva, D; Isus, L; Alfaro, A; Cerbón-Cervantes, M; Aloy, P; Reyes-Chilpa, R

    2016-05-01

    Calophyllum brasiliense (Calophyllaceae) is a tropical rain forest tree distributed in Central and South America. It is an important source of tetracyclic dipyrano coumarins (Soulatrolide) and Mammea type coumarins. Soulatrolide is a potent inhibitor of HIV-1 reverse transcriptase and displays activity against Mycobacterium tuberculosis. Meanwhile, Mammea A/BA and A/BB, pure or as a mixture, are highly active against several human leukemia cell lines, Trypanosoma cruzi and Leishmania amazonensis. Nevertheless, there are few studies evaluating their safety profile. In the present work we performed toxicogenomic and toxicological analysis for both type of compounds. Soulatrolide, and the Mammea A/BA + A/BB mixture (2.1) were slightly toxic accordingly to Lorke assay classification (DL50 > 3000 mg/kg). After a short-term administration (100 mg/kg/daily, orally, 1 week) liver toxicogenomic analysis revealed 46 up and 72 downregulated genes for Mammea coumarins, and 665 up and 1077 downregulated genes for Soulatrolide. Gene enrichment analysis identified transcripts involved in drug metabolism for both compounds. In addition, network analysis through protein-protein interactions, tissue evaluation by TUNEL assay, and histological examination revealed no tissue damage on liver, kidney and spleen after treatments. Our results indicate that both type of coumarins displayed a safety profile, supporting their use in further preclinical studies to determine its therapeutic potential.

  10. Co-stimulation through the CD137/4-1BB pathway protects human melanoma tumor-infiltrating lymphocytes from activation-induced cell death and enhances anti-tumor effector function

    PubMed Central

    Hernandez-Chacon, Jessica Ann; Li, Yufeng; Wu, Richard C.; Bernatchez, Chantale; Wang, Yijun; Weber, Jeffrey; Hwu, Patrick; Radvanyi, Laszlo

    2011-01-01

    Adoptive T-cell therapy (ACT) using expanded tumor-infiltrating lymphocytes (TIL) with high-dose IL-2 is a promising form of immunotherapy for Stage IV melanoma having clinical response rates of 50% or more. One of the major problems preventing further success of this therapy is that the current protocols used to highly expand TIL for infusion drive CD8+ T cells to differentiate into effector cells losing key co-stimulatory molecules such as CD28 and CD27. This has been associated with a lack of persistence in vivo for reasons not entirely clear. In this study, we demonstrate that while human melanoma CD8+ TIL lost CD27 and CD28 expression during the rapid expansion for ACT, they gained expression of the alternative co-stimulatory molecule CD137/4-1BB, and to a lesser extent CD134/OX40. Post-REP TIL were found to be highly sensitive to activation-induced cell death (AICD) when re-activated through the TCR with low levels of OKT3 antibody. However, co-ligation of 4-1BB using two different agonistic anti-4-1BB antibodies potently prevented AICD of post-REP CD8+ TIL, including those specific for MART-1, and facilitated even further cell expansion. This was correlated with increased levels of bcl-2 and bcl-xL together with decreased bim expression. 4-1BB-co-stimulated post-REP TIL also expressed increased levels of the cytolytic granule proteins and exhibited enhanced CTL activity against melanoma cells. Lastly, post-REP CD8+ TIL were protected from cell death by anti-4-1BB ligation when exposed to HLA-matched melanoma cells. Our results indicate that 4-1BB co-stimulation may significantly improve TIL survival during melanoma ACT and boost anti-tumor cytolytic activity. PMID:21389874

  11. Effect of Photobiomodulation on Transforming Growth Factor-β1, Platelet-Derived Growth Factor-BB, and Interleukin-8 Release in Palatal Wounds After Free Gingival Graft Harvesting: A Randomized Clinical Study

    PubMed Central

    Lutfioğlu, Muge; Aydogdu, Ahmet; Saygun, N. Isil; Serdar, Muhittin A.

    2016-01-01

    Abstract Objective: This study evaluated the impact of photobiomodulation (PBM) on the healing of the donor palatal area following free gingival graft (FGG) harvesting by examining changes in transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF)-BB, and interleukin (IL)-8 levels in palatal wound fluid (PWF). Material and methods: Thirty patients were selected and randomly assigned to receive PBM (laser group) or PBM sham (sham group) in the palatine area after FGG harvesting. A neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (1064 nm) was applied to the test sites immediately after surgery and every 24 h thereafter for 4 days. PWF was collected on Days 7 and 12, and PWF TGF-β1, PDGF-BB, and IL-8 levels were analyzed by enzyme-linked immunosorbent assays (ELISA). Results: PWF TGF-β1, PDGF-BB, and IL-8 levels were significantly lower on Day 12 than on Day 7 for both groups. PWF TGF-β1, PDGF-BB, and IL-8 levels of the laser group were significantly higher than those of sham group on Day 7 (p < 0.05). PWF TGF-β1 levels were also significantly higher in laser group than in the sham group on Day 12; however, differences in PDGF-BB and IL-8 levels between groups on Day 12 were statistically nonsignificant. Conclusions: Observed increases in PWF TGF-β1, PDGF-BB, and IL-8 levels suggest that PBM may accelerate wound healing by stimulating production of selected mediators. PMID:27088277

  12. A Small Molecule that Mimics the BB-Loop in the Toll/IL-1 Receptor Domain of MyD88 Attenuates Staphylococcal Enterotoxin B Induced Pro-Inflammatory Cytokine Production and Toxicity in Mice

    DTIC Science & Technology

    2011-06-01

    to allow the cells to swell. 50 µl of 10% Nonidet P - 40 was added to the cells and briefly centrifuged. The nuclear pellet was resuspended in...protein 10 (IP-10). A conserved sequence, (F/Y)-(V/L/I)-( P /G), called the BB- loop appears in the TIR domain of most members of the TLR/IL-1R family...from 7 structural analogs based on the tripeptide sequence of the BB-loop [(F/Y)-(V/L/I)-( P /G)] of the Toll/IL-1 receptor (TIR) domain binding

  13. Cross section for bb¯ production via dielectrons in d + Au collisions at sNN=200 GeV

    DOE PAGES

    Adare, A.; Aidala, C.; Ajitanand, N. N.; ...

    2015-01-26

    We report a measurement of e⁺e⁻ pairs from semileptonic heavy-flavor decays in d+Au collisions at √sNN = 200 GeV. Thus, exploring the mass and transverse-momentum dependence of the yield, the bottom decay contribution can be isolated from charm, and quantified by comparison to PYTHIA and MC@NLO simulations. The resulting bb-production cross section is σdAubb=1.37±0.28(stat)±0.46(syst) mb, which is equivalent to a nucleon-nucleon cross section of σNNbb =3.4 ± 0.8(stat)±1.1(syst) µb.

  14. 1H, 13C, and 15N resonance assignments for the protein coded by gene locus BB0938 of Bordetella bronchiseptica

    SciTech Connect

    Rossi, Paolo; Ramelot, Theresa A.; Xiao, Rong; Ho, Chi K.; Ma, LiChung; Acton, Thomas; Kennedy, Michael A.; Montelione, Gaetano

    2005-11-01

    The product of gene locus BB0938 from Bordetella bronchiseptica (Swiss-Prot ID: Q7WNU7-BORBR; NESG target ID: BoR11; Wunderlich et al., 2004; Pfam ID: PF03476) is a 128-residue protein of unknown function. This broadly conserved protein family is found in eubacteria and eukaryotes. Using triple resonance NMR techniques, we have determined 98% of backbone and 94% of side chain 1H, 13C, and 15N resonance assignments. The chemical shift and 3J(HN?Ha) scalar coupling data reveal a b topology with a seven-residue helical insert, ??????????. BMRB deposit with accession number 6693. Reference: Wunderlich et al. (2004) Proteins, 56, 181?187.

  15. Treatment of a large postextraction buccal wall defect with mineralized allograft, β-TCP, and rhPDGF-BB: a growth factor-mediated bone regenerative approach.

    PubMed

    Snyder, Mark B

    2012-12-01

    Buccal wall defects following tooth removal are frequent in the anterior portions of the mandible and maxilla. Common reasons for such defects include thin buccal bone, preexisting periodontal disease, bundle bone resorption, difficult orthodontic movement, and traumatic extractions. Regeneration of the postextraction defect with vital, well-vascularized, dense bone is critical to a successful implant-supported restoration. This case report examines the effectiveness of using a composite graft of freeze-dried bone allograft and β-tricalcium phosphate plus recombinant human platelet-derived growth factor BB to regenerate healthy, dense bone in a large mandibular anterior buccal wall defect. The importance of access to the overlying periosteum as a readily available source of osteogenic cells in growth factor-mediated bone regenerative procedures is emphasized.

  16. Search for Higgs bosons decaying to bb and produced in association with W bosons in pp collisions at square root of s = 1.96 TeV.

    PubMed

    Abulencia, A; Acosta, D; Adelman, J; Affolder, T; Akimoto, T; Albrow, M G; Ambrose, D; Amerio, S; Amidei, D; Anastassov, A; Anikeev, K; Annovi, A; Antos, J; Aoki, M; Apollinari, G; Arguin, J-F; Arisawa, T; Artikov, A; Ashmanskas, W; Attal, A; Azfar, F; Azzi-Bacchetta, P; Azzurri, P; Bacchetta, N; Bachacou, H; Badgett, W; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Baroiant, S; Bartsch, V; Bauer, G; Bedeschi, F; Behari, S; Belforte, S; Bellettini, G; Bellinger, J; Belloni, A; Ben-Haim, E; Benjamin, D; Beretvas, A; Beringer, J; Berry, T; Bhatti, A; Binkley, M; Bisello, D; Bishai, M; Blair, R E; Blocker, C; Bloom, K; Blumenfeld, B; Bocci, A; Bodek, A; Boisvert, V; Bolla, G; Bolshov, A; Bortoletto, D; Boudreau, J; Bourov, S; Boveia, A; Brau, B; Bromberg, C; Brubaker, E; Budagov, J; Budd, H S; Budd, S; Burkett, K; Busetto, G; Bussey, P; Byrum, K L; Cabrera, S; Campanelli, M; Campbell, M; Canelli, F; Canepa, A; Carlsmith, D; Carosi, R; Carron, S; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chang, S H; Chapman, J; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, I; Cho, K; Chokheli, D; Chou, J P; Chu, P H; Chuang, S H; Chung, K; Chung, W H; Chung, Y S; Ciljak, M; Ciobanu, C I; Ciocci, M A; Clark, A; Clark, D; Coca, M; Connolly, A; Convery, M E; Conway, J; Cooper, B; Copic, K; Cordelli, M; Cortiana, G; Cruz, A; Cuevas, J; Culbertson, R; Cyr, D; DaRonco, S; D'Auria, S; D'onofrio, M; Dagenhart, D; de Barbaro, P; De Cecco, S; Deisher, A; De Lentdecker, G; Dell'Orso, M; Demers, S; Demortier, L; Deng, J; Deninno, M; De Pedis, D; Derwent, P F; Dionisi, C; Dittmann, J R; DiTuro, P; Dörr, C; Dominguez, A; Donati, S; Donega, M; Dong, P; Donini, J; Dorigo, T; Dube, S; Ebina, K; Efron, J; Ehlers, J; Erbacher, R; Errede, D; Errede, S; Eusebi, R; Fang, H C; Farrington, S; Fedorko, I; Fedorko, W T; Feild, R G; Feindt, M; Fernandez, J P; Field, R; Flanagan, G; Flores-Castillo, L R; Foland, A; Forrester, S; Foster, G W; Franklin, M; Freeman, J C; Fujii, Y; Furic, I; Gajjar, A; Gallinaro, M; Galyardt, J; Garcia, J E; Garcia Sciverez, M; Garfinkel, A F; Gay, C; Gerberich, H; Gerchtein, E; Gerdes, D; Giagu, S; di Giovanni, G P; Giannetti, P; Gibson, A; Gibson, K; Ginsburg, C; Giokaris, N; Giolo, K; Giordani, M; Giunta, M; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldschmidt, N; Goldstein, J; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Gotra, Y; Goulianos, K; Gresele, A; Griffiths, M; Grinstein, S; Grosso-Pilcher, C; Grundler, U; Guimaraes da Costa, J; Haber, C; Hahn, S R; Hahn, K; Halkiadakis, E; Hamilton, A; Han, B-Y; Handler, R; Happacher, F; Hara, K; Hare, M; Harper, S; Harr, R F; Harris, R M; Hatakeyama, K; Hauser, J; Hays, C; Hayward, H; Heijboer, A; Heinemann, B; Heinrich, J; Hennecke, M; Herndon, M; Heuser, J; Hidas, D; Hill, C S; Hirschbuehl, D; Hocker, A; Holloway, A; Hou, S; Houlden, M; Hsu, S-C; Huffman, B T; Hughes, R E; Huston, J; Ikado, K; Incandela, J; Introzzi, G; Iori, M; Ishizawa, Y; Ivanov, A; Iyutin, B; James, E; Jang, D; Jayatilaka, B; Jeans, D; Jensen, H; Jeon, E J; Jones, M; Joo, K K; Jun, S Y; Junk, T R; Kamon, T; Kang, J; Karagoz-Unel, M; Karchin, P E; Kato, Y; Kemp, Y; Kephart, R; Kerzel, U; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, M S; Kim, S B; Kim, S H; Kim, Y K; Kirby, M; Kirsch, L; Klimenko, S; Klute, M; Knuteson, B; Ko, B R; Kobayashi, H; Kondo, K; Kong, D J; Konigsberg, J; Kordas, K; Korytov, A; Kotwal, A V; Kovalev, A; Kraus, J; Kravchenko, I; Kreps, M; Kreymer, A; Kroll, J; Krumnack, N; Kruse, M; Krutelyov, V; Kuhlmann, S E; Kusakabe, Y; Kwang, S; Laasanen, A T; Lai, S; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; Lazzizzera, I; Lecci, C; LeCompte, T; Lee, J; Lee, J; Lee, S W; Lefèvre, R; Leonardo, N; Leone, S; Levy, S; Lewis, J D; Li, K; Lin, C; Lin, C S; Lindgren, M; Lipeles, E; Liss, T M; Lister, A; Litvintsev, D O; Liu, T; Liu, Y; Lockyer, N S; Loginov, A; Loreti, M; Loverre, P; Lu, R-S; Lucchesi, D; Lujan, P; Lukens, P; Lungu, G; Lyons, L; Lys, J; Lysak, R; Lytken, E; Mack, P; MacQueen, D; Madrak, R; Maeshima, K; Maksimovic, P; Manca, G; Margaroli, F; Marginean, R; Marino, C; Martin, A; Martin, M; Martin, V; Martínez, M; Maruyama, T; Matsunaga, H; Mattson, M E; Mazini, R; Mazzanti, P; McFarland, K S; McGivern, D; McIntyre, P; McNamara, P; McNulty, R; Mehta, A; Menzemer, S; Menzione, A; Merkel, P; Mesropian, C; Messina, A; von der Mey, M; Miao, T; Miladinovic, N; Miles, J; Miller, R; Miller, J S; Mills, C; Milnik, M; Miquel, R; Miscetti, S; Mitselmakher, G; Miyamoto, A; Moggi, N; Mohr, B; Moore, R; Morello, M; Movilla Fernandez, P; Mülmenstädt, J; Mukherjee, A; Mulhearn, M; Muller, Th; Mumford, R; Murat, P; Nachtman, J; Nahn, S; Nakano, I; Napier, A; Naumov, D; Necula, V; Neu, C; Neubauer, M S; Nielsen, J; Nigmanov, T; Nodulman, L; Norniella, O; Ogawa, T; Oh, S H; Oh, Y D; Okusawa, T; Oldeman, R; Orava, R; Osterberg, K; Pagliarone, C; Palencia, E; Paoletti, R; Papadimitriou, V; Papikonomou, A; Paramonov, A A; Parks, B; Pashapour, S; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Piedra, J; Pitts, K; Plager, C; Pondrom, L; Pope, G; Portell, X; Poukhov, O; Pounder, N; Prakoshyn, F; Pronko, A; Proudfoot, J; Ptohos, F; Punzi, G; Pursley, J; Rademacker, J; Rahaman, A; Rakitin, A; Rappoccio, S; Ratnikov, F; Reisert, B; Rekovic, V; van Remortel, N; Renton, P; Rescigno, M; Richter, S; Rimondi, F; Rinnert, K; Ristori, L; Robertson, W J; Robson, A; Rodrigo, T; Rogers, E; Rolli, S; Roser, R; Rossi, M; Rossin, R; Rott, C; Ruiz, A; Russ, J; Rusu, V; Ryan, D; Saarikko, H; Sabik, S; Safonov, A; Sakumoto, W K; Salamanna, G; Salto, O; Saltzberg, D; Sanchez, C; Santi, L; Sarkar, S; Sato, K; Savard, P; Savoy-Navarro, A; Scheidle, T; Schlabach, P; Schmidt, E E; Schmidt, M P; Schmitt, M; Schwarz, T; Scodellaro, L; Scott, A L; Scribano, A; Scuri, F; Sedov, A; Seidel, S; Seiya, Y; Semenov, A; Semeria, F; Sexton-Kennedy, L; Sfiligoi, I; Shapiro, M D; Shears, T; Shepard, P F; Sherman, D; Shimojima, M; Shochet, M; Shon, Y; Shreyber, I; Sidoti, A; Sill, A; Sinervo, P; Sisakyan, A; Sjolin, J; Skiba, A; Slaughter, A J; Sliwa, K; Smirnov, D; Smith, J R; Snider, F D; Snihur, R; Soderberg, M; Soha, A; Somalwar, S; Sorin, V; Spalding, J; Spinella, F; Squillacioti, P; Stanitzki, M; Staveris-Polykalas, A; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Stuart, D; Suh, J S; Sukhanov, A; Sumorok, K; Sun, H; Suzuki, T; Taffard, A; Tafirout, R; Takashima, R; Takeuchi, Y; Takikawa, K; Tanaka, M; Tanaka, R; Tecchio, M; Teng, P K; Terashi, K; Tether, S; Thom, J; Thompson, A S; Thomson, E; Tipton, P; Tiwari, V; Tkaczyk, S; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Tönnesmann, M; Torre, S; Torretta, D; Tourneur, S; Trischuk, W; Tsuchiya, R; Tsuno, S; Turini, N; Ukegawa, F; Unverhau, T; Uozumi, S; Usynin, D; Vacavant, L; Vaiciulis, A; Vallecorsa, S; Varganov, A; Vataga, E; Velev, G; Veramendi, G; Veszpremi, V; Vickey, T; Vidal, R; Vila, I; Vilar, R; Vollrath, I; Volobouev, I; Würthwein, F; Wagner, P; Wagner, R G; Wagner, R L; Wagner, W; Wallny, R; Walter, T; Wan, Z; Wang, M J; Wang, S M; Warburton, A; Ward, B; Waschke, S; Waters, D; Watts, T; Weber, M; Wester, W C; Whitehouse, B; Whiteson, D; Wicklund, A B; Wicklund, E; Williams, H H; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, C; Worm, S; Wright, T; Wu, X; Wynne, S M; Yagil, A; Yamamoto, K; Yamaoka, J; Yamashita, Y; Yang, C; Yang, U K; Yao, W M; Yeh, G P; Yoh, J; Yorita, K; Yoshida, T; Yu, I; Yu, S S; Yun, J C; Zanello, L; Zanetti, A; Zaw, I; Zetti, F; Zhang, X; Zhou, J; Zucchelli, S

    2006-03-03

    We present a search for Higgs bosons decaying into bb and produced in association with W bosons in pp collisions at square root of s = 1.96 TeV. This search uses 320 pb(-1) of the data set accumulated by the upgraded Collider Detector at Fermilab. Events are selected that have a high-transverse momentum electron or muon, missing transverse energy, and two jets, at least one of which is consistent with the hadronization of a b quark. Both the number of events and the dijet mass distribution are consistent with standard model background expectations, and we set 95% confidence level upper limits on the production cross section times branching ratio for the Higgs boson or any new particle with similar decay kinematics. These upper limits range from 10 pb for mH = 110 GeV/c2 to 3 pb for mH = 150 GeV/c2.

  17. Search for the standard model Higgs boson decaying to a bb pair in events with two oppositely charged leptons using the full CDF data set.

    PubMed

    Aaltonen, T; Álvarez González, B; Amerio, S; Amidei, D; Anastassov, A; Annovi, A; Antos, J; Apollinari, G; Appel, J A; Arisawa, T; Artikov, A; Asaadi, J; Ashmanskas, W; Auerbach, B; Aurisano, A; Azfar, F; Badgett, W; Bae, T; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Barria, P; Bartos, P; Bauce, M; Bedeschi, F; Behari, S; Bellettini, G; Bellinger, J; Benjamin, D; Beretvas, A; Bhatti, A; Binkley, M E; Bisello, D; Bizjak, I; Bland, K R; Blumenfeld, B; Bocci, A; Bodek, A; Bortoletto, D; Boudreau, J; Boveia, A; Brigliadori, L; Bromberg, C; Brucken, E; Budagov, J; Budd, H S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Calamba, A; Calancha, C; Camarda, S; Campanelli, M; Campbell, M; Canelli, F; Carls, B; Carlsmith, D; Carosi, R; Carrillo, S; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavaliere, V; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, K; Chokheli, D; Chung, W H; Chung, Y S; Ciocci, M A; Clark, A; Clarke, C; Compostella, G; Convery, M E; Conway, J; Corbo, M; Cordelli, M; Cox, C A; Cox, D J; Crescioli, F; Cuevas, J; Culbertson, R; Dagenhart, D; d'Ascenzo, N; Datta, M; de Barbaro, P; Dell'Orso, M; Demortier, L; Deninno, M; Devoto, F; d'Errico, M; Di Canto, A; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Donati, S; Dong, P; Dorigo, M; Dorigo, T; Ebina, K; Elagin, A; Eppig, A; Erbacher, R; Errede, S; Ershaidat, N; Eusebi, R; Farrington, S; Feindt, M; Fernandez, J P; Ferrazza, C; Field, R; Flanagan, G; Forrest, R; Frank, M J; Franklin, M; Freeman, J C; Funakoshi, Y; Furic, I; Gallinaro, M; Garcia, J E; Garfinkel, A F; Garosi, P; Gerberich, H; Gerchtein, E; Giagu, S; Giakoumopoulou, V; Giannetti, P; Gibson, K; Ginsburg, C M; Giokaris, N; Giromini, P; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldin, D; Goldschmidt, N; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Grinstein, S; Grosso-Pilcher, C; Group, R C; Guimaraes da Costa, J; Hahn, S R; Halkiadakis, E; Hamaguchi, A; Han, J Y; Happacher, F; Hara, K; Hare, D; Hare, M; Harr, R F; Hatakeyama, K; Hays, C; Heck, M; Heinrich, J; Herndon, M; Hewamanage, S; Hocker, A; Hopkins, W; Horn, D; Hou, S; Hughes, R E; Hurwitz, M; Husemann, U; Hussain, N; Hussein, M; Huston, J; Introzzi, G; Iori, M; Ivanov, A; James, E; Jang, D; Jayatilaka, B; Jeans, D T; Jeon, E J; Jindariani, S; Jones, M; Joo, K K; Jun, S Y; Junk, T R; Kamon, T; Karchin, P E; Kasmi, A; Kato, Y; Ketchum, W; Keung, J; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kim, Y J; Kimura, N; Kirby, M; Klimenko, S; Knoepfel, K; Kondo, K; Kong, D J; Konigsberg, J; Kotwal, A V; Kreps, M; Kroll, J; Krop, D; Kruse, M; Krutelyov, V; Kuhr, T; Kurata, M; Kwang, S; Laasanen, A T; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; LeCompte, T; Lee, E; Lee, H S; Lee, J S; Lee, S W; Leo, S; Leone, S; Lewis, J D; Limosani, A; Lin, C-J; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, C; Liu, H; Liu, Q; Liu, T; Lockwitz, S; Loginov, A; Lucchesi, D; Lueck, J; Lujan, P; Lukens, P; Lungu, G; Lys, J; Lysak, R; Madrak, R; Maeshima, K; Maestro, P; Malik, S; Manca, G; Manousakis-Katsikakis, A; Margaroli, F; Marino, C; Martínez, M; Mastrandrea, P; Matera, K; Mattson, M E; Mazzacane, A; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Mesropian, C; Miao, T; Mietlicki, D; Mitra, A; Miyake, H; Moed, S; Moggi, N; Mondragon, M N; Moon, C S; Moore, R; Morello, M J; Morlock, J; Movilla Fernandez, P; Mukherjee, A; Muller, Th; Murat, P; Mussini, M; Nachtman, J; Nagai, Y; Naganoma, J; Nakano, I; Napier, A; Nett, J; Neu, C; Neubauer, M S; Nielsen, J; Nodulman, L; Noh, S Y; Norniella, O; Oakes, L; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Orava, R; Ortolan, L; Pagan Griso, S; Pagliarone, C; Palencia, E; Papadimitriou, V; Paramonov, A A; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Pianori, E; Pilot, J; Pitts, K; Plager, C; Pondrom, L; Poprocki, S; Potamianos, K; Prokoshin, F; Pranko, A; Ptohos, F; Punzi, G; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Renton, P; Rescigno, M; Riddick, T; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rodriguez, T; Rogers, E; Rolli, S; Roser, R; Ruffini, F; Ruiz, A; Russ, J; Rusu, V; Safonov, A; Sakumoto, W K; Sakurai, Y; Santi, L; Sato, K; Saveliev, V; Savoy-Navarro, A; Schlabach, P; Schmidt, A; Schmidt, E E; Schwarz, T; Scodellaro, L; Scribano, A; Scuri, F; Seidel, S; Seiya, Y; Semenov, A; Sforza, F; Shalhout, S Z; Shears, T; Shepard, P F; Shimojima, M; Shochet, M; Shreyber-Tecker, I; Simonenko, A; Sinervo, P; Sliwa, K; Smith, J R; Snider, F D; Soha, A; Sorin, V; Song, H; Squillacioti, P; Stancari, M; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Strycker, G L; Sudo, Y; Sukhanov, A; Suslov, I; Takemasa, K; Takeuchi, Y; Tang, J; Tecchio, M; Teng, P K; Thom, J; Thome, J; Thompson, G A; Thomson, E; Tipton, P; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Totaro, P; Trovato, M; Ukegawa, F; Uozumi, S; Varganov, A; Vázquez, F; Velev, G; Vellidis, C; Vidal, M; Vila, I; Vilar, R; Vizán, J; Vogel, M; Volpi, G; Wagner, P; Wagner, R L; Wakisaka, T; Wallny, R; Wang, S M; Warburton, A; Waters, D; Wester, W C; Whiteson, D; Wicklund, A B; Wicklund, E; Wilbur, S; Wick, F; Williams, H H; Wilson, J S; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, H; Wright, T; Wu, X; Wu, Z; Yamamoto, K; Yamato, D; Yang, T; Yang, U K; Yang, Y C; Yao, W-M; Yeh, G P; Yi, K; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanetti, A; Zeng, Y; Zhou, C; Zucchelli, S

    2012-09-14

    We present a search for the standard model Higgs boson produced in association with a Z boson in data collected with the CDF II detector at the Tevatron, corresponding to an integrated luminosity of 9.45  fb(-1). In events consistent with the decay of the Higgs boson to a bottom-quark pair and the Z boson to electron or muon pairs, we set 95% credibility level upper limits on the ZH production cross section times the H→bb branching ratio as a function of Higgs boson mass. At a Higgs boson mass of 125  GeV/c(2), we observe (expect) a limit of 7.1 (3.9) times the standard model value.

  18. Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification.

    PubMed

    Huang, Ke-Jing; Shuai, Hong-Lei; Zhang, Ji-Zong

    2016-03-15

    A highly sensitive and ultrasensitive electrochemical aptasensor for platelet-derived growth factor BB (PDGF-BB) detection is fabricated based on layered molybdenum selenide-graphene (MoSe2-Gr) composites and Exonuclease III (Exo III)-aided signal amplification. MoSe2-Gr is prepared by a simple hydrothermal method and used as a promising sensing platform. Exo III has a specifical exo-deoxyribonuclease activity for duplex DNAs in the direction from 3' to 5' terminus, however its activity is limited on the duplex DNAs with more than 4 mismatched terminal bases at 3' ends. Herein, aptamer and complementary DNA (cDNA) sequences are designed with four thymine bases on 3' ends. In the presence of target protein, the aptamer associates with it and facilitates the formation of duplex DNA between cDNA and signal DNA. The duplex DNA then is digested by Exo III and releases cDNA, which hybridizes with signal DNA to perform a new cleavage process. Nevertheless, in the absence of target protein, the aptamer hybridizes with cDNA will inhibit the Exo III-assisted nucleotides cleavage. The signal DNA then hybridizes with capture DNA on the electrode. Subsequently, horse radish peroxidase is fixed on electrode by avidin-biotin reaction and then catalyzes hydrogen peroxide and hydroquinone to produce electrochemical response. Therefore, a bridge can be established between the concentration of target protein and the degree of the attenuation of the obtained signal, providing a quantitative measure of target protein with a broad detection range of 0.0001-1 nM and a detection limit of 20 fM.

  19. Treatment of periodontal intrabony defects using β-TCP alone or in combination with rhPDGF-BB: a randomized controlled clinical and radiographic study.

    PubMed

    Maroo, Sneha; Murthy, K Raja V

    2014-01-01

    The need to increase the predictability of periodontal regeneration has encouraged clinicians and researchers to employ cell-stimulating proteins in combination with osteoconductive scaffolds, based on the principles of tissue engineering. The purpose of this clinical and radiographic study was to compare the regenerative potential of the combination of β-tricalcium phosphate (β-TCP) and recombinant human platelet-derived growth factor BB (rhPDGF-BB) in the grafting of intraosseous defects with the established technique of bone grafting with (β-TCP alone. A total of 30 sites from 15 patients with infrabony defects in two different quadrants were selected, and the sites were randomly divided into test sites (rhPDGF + β-TCP) and control sites (β-TCP alone) using a split-mouth design. Clinical parameters, including probing pocket depth, clinical attachment level, and gingival recession, were recorded at baseline, 6 months, and 9 months. Radiographic evaluation was carried out to evaluate defect fill, change in alveolar crest height, and percentage of defect fill at baseline, 6 months, and 9 months. Both the experimental groups showed statistically significant reduction in probing pocket depth and gain in clinical attachment level. On intergroup comparison, sites treated with rhPDGF + β-TCP demonstrated a significantly greater pocket depth reduction (P < .05) and greater gain in clinical attachment level (P < .01). Mean percentage defect fill was significantly greater in test sites as compared with control sites at 6 and 9 months (P < .01). rhPDGF + β-TCP-treated sites demonstrated a significant gain in mean alveolar crest height at 6 and 9 months (P < .05), while β-TCP-treated sites demonstrated crestal resorption. Both groups demonstrated potential in enhancing periodontal regeneration; however, on comparison between the two groups, the results obtained by rhPDGF + β-TCP were significantly better with respect to both clinical and radiographic parameters.

  20. Human corneal fibroblast migration and ECM synthesis during stromal repair: Role played by PDGF-BB, bFGF, and TGFβ1.

    PubMed

    Gallego-Muñoz, Patricia; Ibares-Frías, Lucía; Garrote, José A; Valsero-Blanco, María Cruz; Cantalapiedra-Rodríguez, Roberto; Merayo-Lloves, Jesús; Martínez-García, M Carmen

    2016-11-15

    The development of treatments that modulate corneal wound healing to avoid fibrosis during tissue repair is important for the restoration of corneal transparency after an injury. To date, few studies have studied the influence of growth factors (GFs) on human corneal fibroblast (HCF) expression of extracellular matrix (ECM) proteins such as collagen types I and III, proteoglycans such as perlecan, or proteins implicated in cellular migration such as α5β1-integrin and syndecan-4. Using in vitro HCFs, we developed a mechanical wound model to study the influence of the GFs basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB), and transforming growth factor beta 1 (TGFβ1) on ECM protein production and cellular migration. Our results show that mechanical wounding provokes the autocrine release of bFGF and TGFβ1 at different time points during the wound closure. The HCF response to PDGF-BB was a rapid closure due to fast cellular migration associated with a high focal adhesion replacement and a high expression of collagen and proteoglycans, producing a non-fibrotic healing. bFGF stimulated non-fibrotic ECM production and limited the migration process. Finally, TGFβ1 induced expression of the fibrotic markers collagen type III and α5β1 integrin, and it inhibited cellular migration due to the formation of focal adhesions with a low turnover rate. The novel in vitro HCF mechanical wound model can be used to understand the role played by GFs in human corneal repair. The model can also be used to test the effects of different treatments aimed at improving the healing process.

  1. Decomposition rates and residue-colonizing microbial communities of Bacillus thuringiensis insecticidal protein Cry3Bb-expressing (Bt) and non-Bt corn hybrids in the field.

    PubMed

    Xue, Kai; Serohijos, Raquel C; Devare, Medha; Thies, Janice E

    2011-02-01

    Despite the rapid adoption of crops expressing the insecticidal Cry protein(s) from Bacillus thuringiensis (Bt), public concern continues to mount over the potential environmental impacts. Reduced residue decomposition rates and increased tissue lignin concentrations reported for some Bt corn hybrids have been highlighted recently as they may influence soil carbon dynamics. We assessed the effects of MON863 Bt corn, producing the Cry3Bb protein against the corn rootworm complex, on these aspects and associated decomposer communities by terminal restriction fragment length polymorphism (T-RFLP) analysis. Litterbags containing cobs, roots, or stalks plus leaves from Bt and unmodified corn with (non-Bt+I) or without (non-Bt) insecticide applied were placed on the soil surface and at a 10-cm depth in field plots planted with these crop treatments. The litterbags were recovered and analyzed after 3.5, 15.5, and 25 months. No significant effect of treatment (Bt, non-Bt, and non-Bt+I) was observed on initial tissue lignin concentrations, litter decomposition rate, or bacterial decomposer communities. The effect of treatment on fungal decomposer communities was minor, with only 1 of 16 comparisons yielding separation by treatment. Environmental factors (litterbag recovery year, litterbag placement, and plot history) led to significant differences for most measured variables. Combined, these results indicate that the differences detected were driven primarily by environmental factors rather than by any differences between the corn hybrids or the use of tefluthrin. We conclude that the Cry3Bb corn tested in this study is unlikely to affect carbon residence time or turnover in soils receiving these crop residues.

  2. Analysis of the CD2 and spliceosomal Sm B/B' polyproline-arginine motifs defined by a monoclonal antibody using a phage-displayed random peptide library.

    PubMed

    Monos, Dimitri; Heliopoulos, John; Argyris, Elias; Cordopatis, Paul; Zompra, Aikaterini; Kamoun, Malek

    2006-01-01

    The cytoplasmic region of the CD2 receptor of lymphocytes contains proline-rich motifs, which are involved in T cell activation and interleukin-2 production. An intracellular CD2 binding protein, CD2BP2, interacts with two tandem PPPPGHR segments of the CD2 tail. CD2BP2 contains a GYF (glycine-tyrosine-phenylalanine) domain that confers binding to these proline-rich sequences. Monoclonal antibody 3E10 that was previously raised against a peptide containing the CD2 PPPPGHR segment reacts with the native CD2 molecule and spliceosomal Sm B/B' proteins. To identify the exact epitope on the CD2 peptide recognized by 3E10, a phage-displayed combinatorial peptide library was used. Analysis of the selected clones revealed that the mAb 3E10 binds preferentially to the motif PxxPPGxR. Experiments using amino acid substitutions with synthetic peptides confirmed the reactivity of mAb 3E10 with this motif. In addition, we show that several similarities exist between this motif and the CD2BP2-GFY recognition motif PPGxR/K. Binding of antibody 3E10 indicates some degree of degeneracy, which is consistent with its ability to recognize structurally related polyproline-arginine motifs found in intracellular proteins including Sm B/B' proteins and other RNA binding proteins. Thus, mAb 3E10 can be used to specifically identify a sub-class of proline-rich motifs, and as such can be used to study the potential role of these proline-rich sequences in mediating protein-protein interactions.

  3. Polybrominated diphenyl ethers (PBDEs) and 2,2',4,4',5,5'-hexabromobiphenyl (BB-153) in landfill leachate in Cape Town, South Africa.

    PubMed

    Daso, Adegbenro P; Fatoki, Olalekan S; Odendaal, James P; Olujimi, Olanrewaju O

    2013-01-01

    An assessment of the concentrations of selected polybrominated diphenyl ether (PBDE) congeners as well as BB 153 in leachate samples collected from three landfill sites within the city of Cape Town was conducted. A liquid-liquid extraction technique was employed for the isolation of all the target compounds from the leachate samples. Extracts obtained were further subjected to multi-layer column chromatography employing different forms of silica gel. The prepared samples were analysed using a high capillary gas chromatograph equipped with a micro-electron capture detector (GC-μECD). The overall mean concentrations of the total PBDEs, including BDE 209 ranged between 5.65 and 2,240, 0.28-20.5 and 1.66-1,170 ng/l for Bellville, Coastal Park, and Vissershok landfill sites, respectively. The mean concentrations of BB 153, which were generally low in most of the samples analysed, were 70.4, 7.14 and 8.16 ng/l for Bellville, Coastal Park and Vissershok sites, respectively. The influence of precipitation on the characteristics and quantity of leachate produced from the landfill sites investigated was most pronounced during the August/September sampling regime. Generally, the trend observed in this study clearly indicated a wide variation in the levels of these contaminants in all the landfill sites studied from one sampling period to the other. However, the principal component analysis revealed that the release of these contaminants might be associated with two or three possible sources. This study further confirmed the relevance of landfill leachate as an important source of PBDE contamination of the environment, especially the groundwater and surface water sources.

  4. The broad-spectrum metalloproteinase inhibitor BB-94 inhibits growth, HER3 and Erk activation in fulvestrant-resistant breast cancer cell lines.

    PubMed

    Kirkegaard, Tove; Yde, Christina W; Kveiborg, Marie; Lykkesfeldt, Anne E

    2014-07-01

    Breast cancer cells can switch from estrogen receptor α (ER)- to human epidermal growth factor receptor (HER)-driven cell growth upon acquiring antiestrogen resistance. HER ligands are cleaved by metalloproteinases leading to release of active HER ligands, activation of HER receptors and consequently increased cell growth. In this study, we investigated the importance of HER receptors, in particular HER3, and HER ligand shedding for growth and signaling in human MCF-7 breast cancer cells and MCF-7-derived sublines resistant to the antiestrogen fulvestrant. The HER3/HER4 ligand heregulin 1β induced phosphorylation of HER3, Akt and Erk, and partly rescued fulvestrant-inhibited growth of MCF-7 cells. HER3 ligands were found to be produced and shed from the fulvestrant-resistant cells as conditioned medium from fulvestrant-resistant MCF-7 cells induced phosphorylation of HER3 and Akt in MCF-7 cells. This was prevented by treatment of resistant cells with the metalloproteinase inhibitor TAPI-2. Only the broad-spectrum metalloproteinase inhibitor BB-94, and not the more selective inhibitors GM6001 or TAPI-2, which inhibited shedding of the HER ligands produced by the fulvestrant-resistant cells, was able to inhibit growth and activation of HER3 and Erk in resistant cells. Compared to MCF-7, fulvestrant-resistant cells have increased HER3 phosphorylation, but knockdown of HER3 had no inhibitory effect on resistant cell growth. The EGFR inhibitor gefitinib exhibited only a minor growth inhibition, whereas the pan-HER inhibitor CI-1033 exerted growth arrest. Thus, neither HER3 nor EGFR alone are the main driver of fulvestrant-resistant cell growth and treatment should target both receptors. Ligand shedding is not a treatment target, as receptor activation occurred, independent of release of ligands. Only the broad-spectrum metalloproteinase inhibitor BB-94 could abrogate HER3 and Erk activation in the resistant cells, which stresses the complexity of the resistance

  5. Evaluation of the state-of-the-art measurement capabilities for selected PBDEs and decaBB in plastic by the international intercomparison CCQM-P114.

    PubMed

    Zeleny, R; Voorspoels, S; Ricci, M; Becker, R; Jung, C; Bremser, W; Sittidech, M; Panyawathanakit, N; Wong, W F; Choi, S M; Lo, K C; Yeung, W Y; Kim, D H; Han, J; Ryu, J; Mingwu, S; Chao, W; Schantz, M M; Lippa, K A; Matsuyama, S

    2010-02-01

    An international intercomparison involving eight national metrology institutes (NMIs) was conducted to establish their current measurement capabilities for determining five selected congeners from the brominated flame retardant classes polybrominated diphenyl ethers and polybrominated biphenyls. A candidate reference material consisting of polypropylene fortified with technical mixtures of penta-, octa- and decabromo diphenyl ether and decabromo biphenyl, which was thoroughly assessed for material homogeneity and stability, was used as study material. The analytical procedures applied by the participants differed with regard to sample pre-treatment, extraction, clean-up, employed calibrants and type of calibration procedure as well as regarding analytical methods used for separation, identification and quantification of the flame retardant congeners (gas chromatography coupled to an electron capture detector (GC-ECD), gas chromatography-mass spectrometry in the electron ionisation mode (GC-EI-MS), gas chromatography-mass spectrometry in the electron capture negative ionisation mode (GC-ECNI-MS), and liquid chromatography-inductive coupled plasma-mass spectrometry (LC-ICP-MS)). The laboratory means agreed well with relative standard deviations of the mean of means of 1.9%, 4.8%, 5.5% and 5.4% for brominated diphenyl ether (BDE) 47, 183 and 209 and for the brominated biphenyl (BB) congener 209, respectively. For BDE 206, a relative standard deviation of 28.5% was obtained. For all five congeners, within-laboratory relative standard deviations of six measurements obtained under intermediate precision conditions were between 1% and 10%, and reported expanded measurements uncertainties typically ranged from 4% to 10% (8% to 14% for BDE 206). Furthermore, the results are in good agreement with those obtained in the characterization exercise for determining certified values for the flame retardant congeners in the same material. The results demonstrate the state

  6. Combined Search for the Standard Model Higgs Boson Decaying to bb̄ Using the D0 Run II Data Set

    DOE PAGES

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; ...

    2012-09-20

    We present the results of the combination of searches for the standard model Higgs boson produced in association with a W or Z boson and decaying into bb̄ using the data sample collected with the D0 detector in pp̄ collisions at √s=1.96 TeV at the Fermilab Tevatron Collider. We derive 95% C.L. upper limits on the Higgs boson cross section relative to the standard model prediction in the mass range 100 GeV≤MH≤150 GeV, and we exclude Higgs bosons with masses smaller than 102 GeV at the 95% C.L. In the mass range 120 GeV≤MH≤145 GeV, the data exhibit an excessmore » above the background prediction with a global significance of 1.5 standard deviations, consistent with the expectation in the presence of a standard model Higgs boson.« less

  7. TIR/BB-loop mimetic AS-1 attenuates cardiac ischemia/reperfusion injury via a caveolae and caveolin-3-dependent mechanism

    PubMed Central

    Hu, Yuanping; Zhang, Meiling; Shen, Xin; Dai, Guoliang; Ren, Danyang; Que, Linli; Ha, Tuanzhu; Li, Chuanfu; Xu, Yong; Ju, Wenzheng; Li, Yuehua

    2017-01-01

    AS-1, the TIR/BB loop mimetic, plays a protective role in cardiac ischemia/reperfusion (I/R) but the molecular mechanism remains unclear. The muscle specific caveolin3 (Cav-3) and the caveolae have been found to be critical for cardioprotection. This study aimed to evaluate our hypothesis that caveolae and Cav-3 are essential for AS-1-induced cardioprotection against myocardial I/R injury. To address these issues, we analyzed the involvement of Cav-3 in AS-1 mediated cardioprotection both in vivo and in vitro. We demonstrate that AS-1 administration significantly decreased infarct size, improved cardiac function after myocardial I/R and modulated membrane caveolae and Cav-3 expression in the myocardium. For in vitro studies, AS-1 treatment prevented Cav-3 re-distribution induced by H/R injury. In contrast, disruption of caveolae by MCD treatment or Cav-3 knockdown abolished the protection against H/R-induced myocytes injury by AS-1. Our findings reveal that AS-1 attenuates myocardial I/R injury through caveolae and Cav-3 dependent mechanism. PMID:28291255

  8. Microencapsulation of L. acidophilus (La-05) and B. lactis (Bb-12) and evaluation of their survival at the pH values of the stomach and in bile.

    PubMed

    Fávaro-Trindade, C S; Grosso, C R F

    2002-01-01

    Microcapsules were prepared using the probiotic microorganisms Lactobacillus acidophilus (La-05) and Bifidobacterium lactis (Bb-12) and the spray drying technique and cellulose acetate phthalate as the wall material. This study evaluated the resistance of these microorganisms to drying at three temperatures and also the in vitro tolerance of the free and microencapsulated form to pH values and bile concentrations similar to those found in the human stomach and intestine. With an air entry temperature of 130 degrees C and exit temperature of 75 degrees C, the number of viable cells of B. lactis was practically unaltered, whereas the population of L. acidophilus was reduced by two logarithmic cycles. B. lactis was more resistant to the drying process than L. acidophilus under all conditions tested. The morphology of the microcapsules was determined by scanning electron microscopy and the microcapsules presented a rounded external surface containing concavities, a continuous wall with no apparent porosity, average size of 22 microm, moisture content varying from 5.3 to 3.2% and water activity between 0.230 and 0.204. After inoculation into HCl solutions with pH values adjusted to 1 and 2, incubated anaerobically at 37 degrees C, and plated after 0, 1 and 2 h of incubation, microcapsules were effective in protecting the microorganisms, while the populations of both free microorganisms were eliminated after only 1 h at the acidic conditions. Microencapsulated B. lactis and L. acidophilus, both free and microencapsulated, were also resistant after 12h to bile solutions.

  9. Combined Search for the Standard Model Higgs Boson Decaying to bb̄ Using the D0 Run II Data Set

    SciTech Connect

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Alverson, G.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Bose, T.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Brown, J.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chakraborty, D.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Chevalier-Théry, S.; Cho, D. K.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M.-C.; Croc, A.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duggan, D.; Duperrin, A.; Dutt, S.; Dyshkant, A.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, A.; Evdokimov, V. N.; Facini, G.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garcia-Bellido, A.; García-González, J. A.; García-Guerra, G. A.; Gavrilov, V.; Gay, P.; Geng, W.; Gerbaudo, D.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Golovanov, G.; Goussiou, A.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J.-F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Hagopian, S.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kaadze, K.; Kajfasz, E.; Karmanov, D.; Kasper, P. A.; Katsanos, I.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kulikov, S.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Landsberg, G.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Lubatti, H. J.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Maravin, Y.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Naimuddin, M.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Padilla, M.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M.-A.; Podesta-Lerma, P. L. M.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Rangel, M. S.; Ranjan, K.; Ratoff, P. N.; Razumov, I.; Renkel, P.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Salcido, P.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schlobohm, S.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Shivpuri, R. K.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Smith, K. J.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Takahashi, M.; Titov, M.; Tokmenin, V. V.; Tsai, Y.-T.; Tschann-Grimm, K.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verdier, P.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Wang, R.-J.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; White, A.; Wicke, D.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yang, W.-C.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2012-09-20

    We present the results of the combination of searches for the standard model Higgs boson produced in association with a W or Z boson and decaying into bb̄ using the data sample collected with the D0 detector in pp̄ collisions at √s=1.96 TeV at the Fermilab Tevatron Collider. We derive 95% C.L. upper limits on the Higgs boson cross section relative to the standard model prediction in the mass range 100 GeV≤MH≤150 GeV, and we exclude Higgs bosons with masses smaller than 102 GeV at the 95% C.L. In the mass range 120 GeV≤MH≤145 GeV, the data exhibit an excess above the background prediction with a global significance of 1.5 standard deviations, consistent with the expectation in the presence of a standard model Higgs boson.

  10. Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line.

    PubMed

    Rungrojsakul, Methee; Saiai, Aroonchai; Ampasavate, Chadarat; Anuchapreeda, Songyot; Okonogi, Siriporn

    2016-01-01

    Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription factor that stimulates cell proliferation. The ethanol saraphi seed (ESS) extract was fractionated using n-hexane, ethyl acetate, n-butanol and water to obtain n-hexane saraphi seed (HSS), ethyl acetate saraphi seed (EASS), n-butanol saraphi seed (BSS), and water saraphi seed (WSS) extracts, respectively. The ESS, HSS and EASS extracts had strong cytotoxic effects on K562 cells in the MTT assay. All three fractions decreased WT1 protein levels and decreased total cell numbers. The HSS extract decreased the WT1 protein levels in a time- and dose-dependent manner. HPLC and NMR analyses indicated that the active compound of HSS was mammea E/BB. M. siamensis seeds are thus identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression.

  11. Generation and characterization of ABBV642, a dual variable domain immunoglobulin molecule (DVD-Ig) that potently neutralizes VEGF and PDGF-BB and is designed for the treatment of exudative age-related macular degeneration.

    PubMed

    Ding, Kun; Eaton, Lucia; Bowley, Diana; Rieser, Matthew; Chang, Qing; Harris, Maria C; Clabbers, Anca; Dong, Feng; Shen, Jikui; Hackett, Sean F; Touw, Debra S; Bixby, Jacqueline; Zhong, Suju; Benatuil, Lorenzo; Bose, Sahana; Grinnell, Christine; Preston, Gregory M; Iyer, Ramesh; Sadhukhan, Ramkrishna; Marchie, Susan; Overmeyer, Gary; Ghayur, Tariq; van Riet, Deborah A; Tang, Shibo; Campochario, Peter A; Gu, Jijie

    Exudative age-related macular degeneration (AMD) is the most common cause of moderate and severe vision loss in developed countries. Intraocular injections of vascular endothelial growth factor (VEGF or VEGF-A)-neutralizing proteins provide substantial benefit, but frequent, long-term injections are needed. In addition, many patients experience initial visual gains that are ultimately lost due to subretinal fibrosis. Preclinical studies and early phase clinical trials suggest that combined suppression of VEGF and platelet-derived growth factor-BB (PDGF-BB) provides better outcomes than suppression of VEGF alone, due to more frequent regression of neovascularization (NV) and suppression of subretinal fibrosis. We generated a dual variable domain immunoglobulin molecule, ABBV642 that specifically and potently binds and neutralizes VEGF and PDGF-BB. ABBV642 has been optimized for treatment of exudative AMD based on the following design characteristics: 1) high affinity binding to all VEGF-A isoforms and both soluble and extracellular matrix (ECM)-associated PDGF-BB; 2) potential for extended residence time in the vitreous cavity to decrease the frequency of intraocular injections; 3) rapid clearance from systemic circulation compared with molecules with wild type Fc region for normal FcRn binding, which may reduce the risk of systemic complications; and 4) low risk of potential effector function. The bispecificity of ABBV642 allows for a single injection of a single therapeutic agent, and thus a more streamlined development and regulatory path compared with combination products. In a mouse model of exudative AMD, ABBV642 was observed to be more effective than aflibercept. ABBV642 has potential to improve efficacy with reduced injection frequency in patients with exudative AMD, thereby reducing the enormous disease burden for patients and society.

  12. Influence of the addition of Lactobacillus acidophilus La-05, Bifidobacterium animalis subsp. lactis Bb-12 and inulin on the technological, physicochemical, microbiological and sensory features of creamy goat cheese.

    PubMed

    Barbosa, Ilsa C; Oliveira, Maria E G; Madruga, Marta S; Gullón, Beatriz; Pacheco, Maria T B; Gomes, Ana M P; Batista, Ana S M; Pintado, Maria M E; Souza, Evandro L; Queiroga, Rita C R E

    2016-10-12

    The effects of the addition of Lactobacillus acidophilus LA-05, Bifidobacterium animalis subsp. lactis BB-12 and inulin on the quality characteristics of creamy goat cheese during refrigerated storage were evaluated. The manufactured cheeses included the addition of starter culture (Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris - R-704) (CC); starter culture, L. acidophilus LA-05 and inulin (CLA); starter culture, B. lactis BB-12 and inulin (CBB); or starter culture, L. acidophilus LA-05, B. lactis BB-12 and inulin (CLB). In the synbiotic cheeses (CLA, CBB and CLB), the counts of L. acidophilus LA-05 and B. lactis BB-12 were greater than 6log CFU g(-1), the amount of inulin was greater than 6 g per 100 g, and the firmness was reduced. The cheeses evaluated had high brightness values (L*), with a predominance of yellow (b*). CC had higher contents of proteins, lipids and minerals compared to the other cheeses. There was a decrease in the amount of short-chain fatty acids (SCFAs) and an increase of medium-chain (MCFAs) and long-chain fatty acids (LCFAs) in the synbiotic cheeses compared to CC. The amount of conjugated linoleic acid increased in CLA, CBB and CLB. The highest depth of proteolysis and the greatest changes in the release of free amino acids were found in CLB. The addition of inulin and probiotics, alone or in co-culture, did not affect the cheese acceptance. Inulin and probiotics can be used together for the production of creamy goat cheese without negatively affecting the general quality characteristics of the product, and to add value because of its synbiotic potential.

  13. Effect of the probiotic strain Bifidobacterium animalis subsp. lactis, BB-12®, on defecation frequency in healthy subjects with low defecation frequency and abdominal discomfort: a randomised, double-blind, placebo-controlled, parallel-group trial.

    PubMed

    Eskesen, Dorte; Jespersen, Lillian; Michelsen, Birgit; Whorwell, Peter J; Müller-Lissner, Stefan; Morberg, Cathrine M

    2015-11-28

    The aim of the present study was to investigate the effect of Bifidobacterium animalis subsp. lactis, BB-12®, on two primary end points - defecation frequency and gastrointestinal (GI) well-being - in healthy adults with low defecation frequency and abdominal discomfort. A total of 1248 subjects were included in a randomised, double-blind, placebo-controlled trial. After a 2-week run-in period, subjects were randomised to 1 or 10 billion colony-forming units/d of the probiotic strain BB-12® or a matching placebo capsule once daily for 4 weeks. Subjects completed a diary on bowel habits, relief of abdominal discomfort and symptoms. GI well-being, defined as global relief of abdominal discomfort, did not show significant differences. The OR for having a defecation frequency above baseline for ≥50% of the time was 1·31 (95% CI 0·98, 1·75), P=0·071, for probiotic treatment overall. Tightening the criteria for being a responder to an increase of ≥1 d/week for ≥50 % of the time resulted in an OR of 1·55 (95% CI 1·22, 1·96), P=0·0003, for treatment overall. A treatment effect on average defecation frequency was found (P=0·0065), with the frequency being significantly higher compared with placebo at all weeks for probiotic treatment overall (all P<0·05). Effects on defecation frequency were similar for the two doses tested, suggesting that a ceiling effect was reached with the one billion dose. Overall, 4 weeks' supplementation with the probiotic strain BB-12® resulted in a clinically relevant benefit on defecation frequency. The results suggest that consumption of BB-12® improves the GI health of individuals whose symptoms are not sufficiently severe to consult a doctor (ISRCTN18128385).

  14. Impact of the Bt Corn Proteins Cry34/35Ab1 and Cry3Bb1, Alone or Pyramided, on Western Corn Rootworm (Coleoptera: Chrysomelidae) Beetle Emergence in the Field.

    PubMed

    Hitchon, A J; Smith, J L; French, B W; Schaafsma, A W

    2015-08-01

    Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a major pest of corn, Zea mays L. The effect of the Bt proteins Cry34/35Ab1 and Cry3Bb1, alone or pyramided in corn hybrids on D. v. virgifera adult emergence was evaluated in field experiments for 3 yr. Experiments were infested artificially with 2,500 viable D. v. virgifera eggs per row meter of corn. The reduction in beetle emergence compared with non-Bt controls, from Cry34/35Ab1, Cry3Bb1, and the pyramided hybrids ranged from 64.3 to 97.4%, 91.1 to 95.2%, and 98.1 to 99.6%, respectively. The sex ratio of emerged beetles was usually female-biased from the Cry3Bb1 and pyramided treatments, but not from Cry34/35Ab1 treatment alone. Emergence from all Bt hybrids was delayed compared with the control, with the delay longest from the pyramided hybrid. In 2013, three egg infestation levels were tested, with density-dependent mortality observed at 1,250 viable eggs per row meter. The effect of Bt proteins on the emergence timing and sex ratio of D. v. virgifera may impact the suitability of resistance management plans, specifically the effectiveness of the refuge strategy. Susceptible males emerging from refuge might not be synchronized to mate with potentially resistant females emerging later from Bt corn hybrids.

  15. Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota.

    PubMed

    Martinez, Rafael C R; Cardarelli, Haíssa R; Borst, Wendy; Albrecht, Simone; Schols, Henk; Gutiérrez, Odette P; Maathuis, Annet J H; de Melo Franco, Bernadette D G; De Martinis, Elaine C P; Zoetendal, Erwin G; Venema, Koen; Saad, Susana M I; Smidt, Hauke

    2013-04-01

    A validated in vitro model of the large intestine (TIM-2), set up with human or pig faeces, was used to evaluate the impact of potentially probiotic Lactobacillus amylovorus DSM 16698, administered alone (i), in the presence of prebiotic galactooligosaccharides (GOS) (ii), and co-administered with probiotic Bifidobacterium animalis ssp. lactis Bb-12 (Bb-12) (iii) on GOS degradation, microbial growth (L. amylovorus, lactobacilli, bifidobacteria and total bacteria) and metabolite production. High performance anion exchange chromatography revealed that GOS degradation was more pronounced in TIM-2 inoculated with pig faeces than with human faeces. Denaturing gradient gel electrophoresis profiling of PCR-amplified 16S rRNA genes detected a more complex Lactobacillus spp. community in pig faecal material than in human faecal inoculum. According to 16S rRNA gene-targeted qPCR, GOS stimulated the growth of lactobacilli and bifidobacteria in faecal material from both materials. The cumulative production of short chain fatty acids and ammonia was higher (P < 0.05) for pig than for human faeces. However, lactate accumulation was higher (P < 0.05) in the human model and increased after co-administration with GOS and Bb-12. This study reinforced the notion that differences in microbiota composition between target host organisms need to be considered when animal data are extrapolated to human, as is often done with pre- and probiotic intervention studies.

  16. Seasonal changes of the ambient noise recorded by the "13 BB star" array in northern Poland within the Trans European Suture Zone

    NASA Astrophysics Data System (ADS)

    Lepore, Simone; Grad, Marek

    2016-04-01

    The variations in the azimuth of ambient noise sources, as well as the coherence of the average velocity of surface waves arrivals, were evaluated by applying beam forming and seismic interferometry techniques to the recordings carried out during 2014 at the "13 BB star" array composed of thirteen broadband seismic stations located in northern Poland within the Trans European Suture Zone. The evaluation of the beam power for the whole array each five days for the horizontal and vertical components led to the estimation of the azimuth variation of noise sources during the entire 2014. Fifty days represent a reasonable period to observe seasonal variations of the azimuth in time. The analysis of the azimuths makes evident the strongest beam power associated to noise does not show a preferred direction. The azimuth is predominantly fluctuating between the North Sea and the Baltic Sea: nevertheless, secondary sources like the Atlantic Sea, the Mediterranean Sea and the Black Sea were also noticed. To put in evidence the seasonal variations, the amplitude associated to the principal source was evaluated for the three components. It shows high values in January, March, April, July, August and November, whereas it is low in the remaining months. The analysis of the crosscorrelation between all the station pairs, obtained from the stacking of daily traces for January, April and September 2014 in the 0.1 1 Hz frequency band, allowed the estimation of precise values of velocities of surface waves. The best resolution to retrieve the surface waves is achieved in April, whereas in January and September several higher modes are still present in the traces. The fastest arrivals of surface waves are between ~7 s at ~20 km distance and ~40 s at ~120 km with an average velocity of ~3 km/s. The second group of arrivals is located between ~10 s at ~20 km distance and ~60 s at ~120 km: accordingly, the average velocity is ~2 km/s. The third group of arrivals, between ~13 s at 20 km

  17. 4-1BB ligand as an effective multifunctional immunomodulator and antigen delivery vehicle for the development of therapeutic cancer vaccines

    PubMed Central

    Sharma, Rajesh K.; Schabowsky, Rich-Henry; Srivastava, Abhishek K.; Elpek, Kutlu G.; Madireddi, Shravan; Zhao, Hong; Zhong, Zhenping; Miller, Robert W.; MacLeod, Kathryn J.; Yolcu, Esma S.; Shirwan, Haval

    2010-01-01

    Therapeutic subunit vaccines based on tumor-associated antigens (TAAs) represent an attractive approach for the treatment of cancer. However, poor immunogenicity of TAAs requires potent adjuvants for therapeutic efficacy. We recently proposed the TNF family costimulatory ligands as potential adjuvants for therapeutic vaccines, and hence generated a soluble form of 4-1BBL chimeric with streptavidin (SA-4-1BBL) that has pleiotropic effects on cells of innate, adaptive, and regulatory immunity. We herein tested whether these effects can translate into effective cancer immunotherapy when SA-4-1BBL was also used as a vehicle to deliver TAAs in vivo to DCs constitutively expressing the 4-1BB receptor. SA-4-1BBL was internalized by DCs upon receptor binding, and immunization with biotinylated antigens conjugated to SA-4-1BBL resulted in increased antigen uptake and cross-presentation by DCs, leading to the generation of effective T cell immune responses. Conjugate vaccines containing human papilloma virus 16 (HPV-16) E7 oncoprotein or survivin as a self TAA had potent therapeutic efficacy against TC-1 cervical and 3LL lung carcinoma tumors, respectively. Therapeutic efficacy of the vaccines was associated with increased CD4+ T and CD8+ T cell effector and memory responses and higher intratumoral CD8+ T effector/CD4+CD25+Foxp3+ T regulatory cell ratio. Thus, potent pleiotropic immune functions of SA-4-1BBL combined with its ability to serve as a vehicle to increase delivery of antigens to DCs in vivo endow this molecule with the potential to serve as an effective immunomodulatory component of therapeutic vaccines against cancer and chronic infections. PMID:20406989

  18. AhR-dependent secretion of PDGF-BB by human classically activated macrophages exposed to DEP extracts stimulates lung fibroblast proliferation.

    PubMed

    Jaguin, Marie; Fardel, Olivier; Lecureur, Valérie

    2015-06-15

    Lung diseases are aggravated by exposure to diesel exhaust particles (DEPs) found in air pollution. Macrophages are thought to play a crucial role in lung immune response to these pollutants, even if the mechanisms involved remain incompletely characterized. In the present study, we demonstrated that classically and alternative human macrophages (MΦ) exhibited increased secretion of PDGF-B in response to DEP extract (DEPe). This occurred via aryl hydrocarbon receptor (AhR)-activation because DEPe-induced PDGF-B overexpression was abrogated after AhR expression knock-down by RNA interference, in both M1 and M2 polarizing MΦ. In addition, TCDD and benzo(a)pyrene, two potent AhR ligands, also significantly increased mRNA expression of PDGF-B in M1 MΦ, whereas some weak ligands of AhR did not. We next evaluated the impact of conditioned media (CM) from MΦ culture exposed to DEPe or of recombinant PDGF-B onto lung fibroblast proliferation. The tyrosine kinase inhibitor, AG-1295, prevents phosphorylations of PDGF-Rβ, AKT and ERK1/2 and the proliferation of MRC-5 fibroblasts induced by recombinant PDGF-B and by CM from M1 polarizing MΦ, strongly suggesting that the PDGF-BB secreted by DEPe-exposed MΦ is sufficient to activate the PDGF-Rβ pathway of human lung fibroblasts. In conclusion, we demonstrated that human MΦ, whatever their polarization status, secrete PDGF-B in response to DEPe and that PDGF-B is a target gene of AhR. Therefore, induction of PDGF-B by DEP may participate in the deleterious effects towards human health triggered by such environmental urban contaminants.

  19. Multiple assays indicate varying levels of cross resistance of Cry3Bb1-selected field populations of the western corn rootworm to mCry3A, eCry3.1Ab, and Cry34/35Ab1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Minnesota populations of the western corn rootworm (WCR) surviving Cry3Bb1-expressing corn in the field and WCR populations assumed to be susceptible to all Bt proteins were evaluated for susceptibility to Cry3Bb1, mCry3A, eCry3.1Ab, and Cry34/35Ab1 in diet assays and three different plant-based ass...

  20. The IberArray BB seismic network of Topo-Iberia: new constraints revealing the deep structure of the Iberian Peninsula and North Morocco

    NASA Astrophysics Data System (ADS)

    Gallart, J.; Diaz Cusi, J.; Villasenor, A.; Mancilla, F. D. L.; Bonatto, L.; Schimmel, M.; El moudnib, L.

    2014-12-01

    Topo-Iberia has been a large-scale Spanish project running from 2007 to 2013 that integrated more than 150 researchers on Earth Sciences. One of its key assets was the management of an observatory platform, named IberArray, aimed to provide new geophysical datasets (seismic, GPS, MT) to constrain the structure of Iberia with unprecedented resolution. The IberArray seismic pool was composed by 70+ BB stations, covering the study area in 3 deployments with a site-density of 60km x 60km. The data base holds ~300 sites, including the permanent networks in the area. Hence it forms a unique seismic database in Europe that allow for multiple analyses to constrain the complex geodinamics of the Western Mediterranean. A summary of new results coming from different techniques is presented here. The SKS splitting analysis has provided a spectacular image of the rotation of the fast velocity direction along the Gibraltar Arc. In central and northern Iberia, the fast polarization directions are close to EW, consistently with global mantle flow models considering contributions of surface plate motion, density variations and net lithosphere rotation. Those results suggest an asthenospheric origin of the observed anisotropy related to present-day mantle flow. Receiver functions have revealed the crustal thickness variations beneath the Rif and southern Iberia, including a crustal root beneath the Rif. The Variscan Iberian massif shows a flat Moho discontinuity, while the areas reworked in the Alpine orogeny show a slightly thicker crust. Beneath N Iberia, the imbrication of the Iberian and Eurasian crusts results in complex receiver functions. Depths exceeding 45 km are observed along the Pyrenean range, while the crust thins to values of 26-28 km close to the Atlantic coasts. The geometry of the 410-km and 660-km discontinuities has been investigated using novel cross-correlation/stacking techniques. Ambient noise tomography allows to identify the main sedimentary basins and to

  1. AhR-dependent secretion of PDGF-BB by human classically activated macrophages exposed to DEP extracts stimulates lung fibroblast proliferation

    SciTech Connect

    Jaguin, Marie; Fardel, Olivier; Lecureur, Valérie

    2015-06-15

    Lung diseases are aggravated by exposure to diesel exhaust particles (DEPs) found in air pollution. Macrophages are thought to play a crucial role in lung immune response to these pollutants, even if the mechanisms involved remain incompletely characterized. In the present study, we demonstrated that classically and alternative human macrophages (MΦ) exhibited increased secretion of PDGF-B in response to DEP extract (DEPe). This occurred via aryl hydrocarbon receptor (AhR)-activation because DEPe-induced PDGF-B overexpression was abrogated after AhR expression knock-down by RNA interference, in both M1 and M2 polarizing MΦ. In addition, TCDD and benzo(a)pyrene, two potent AhR ligands, also significantly increased mRNA expression of PDGF-B in M1 MΦ, whereas some weak ligands of AhR did not. We next evaluated the impact of conditioned media (CM) from MΦ culture exposed to DEPe or of recombinant PDGF-B onto lung fibroblast proliferation. The tyrosine kinase inhibitor, AG-1295, prevents phosphorylations of PDGF-Rβ, AKT and ERK1/2 and the proliferation of MRC-5 fibroblasts induced by recombinant PDGF-B and by CM from M1 polarizing MΦ, strongly suggesting that the PDGF-BB secreted by DEPe-exposed MΦ is sufficient to activate the PDGF-Rβ pathway of human lung fibroblasts. In conclusion, we demonstrated that human MΦ, whatever their polarization status, secrete PDGF-B in response to DEPe and that PDGF-B is a target gene of AhR. Therefore, induction of PDGF-B by DEP may participate in the deleterious effects towards human health triggered by such environmental urban contaminants. - Highlights: • PDGF-B expression and secretion are increased by DEPe exposure in human M1 and M2 MΦ. • DEPe-induced PDGF-B expression is aryl-hydrocarbon-dependent. • DEPe-exposed M1 MΦ secrete sufficient PDGF-B to increase lung fibroblast proliferation.

  2. THE RADIAL VELOCITY DETECTION OF EARTH-MASS PLANETS IN THE PRESENCE OF ACTIVITY NOISE: THE CASE OF {alpha} CENTAURI Bb

    SciTech Connect

    Hatzes, Artie P.

    2013-06-20

    We present an analysis of the publicly available HARPS radial velocity (RV) measurements for {alpha} Cen B, a star hosting an Earth-mass planet candidate in a 3.24 day orbit. The goal is to devise robust ways of extracting low-amplitude RV signals of low-mass planets in the presence of activity noise. Two approaches were used to remove the stellar activity signal which dominates the RV variations: (1) Fourier component analysis (pre-whitening), and (2) local trend filtering (LTF) of the activity using short time windows of the data. The Fourier procedure results in a signal at P = 3.236 days and K = 0.42 m s{sup -1}, which is consistent with the presence of an Earth-mass planet, but the false alarm probability for this signal is rather high at a few percent. The LTF results in no significant detection of the planet signal, although it is possible to detect a marginal planet signal with this method using a different choice of time windows and fitting functions. However, even in this case the significance of the 3.24 day signal depends on the details of how a time window containing only 10% of the data is filtered. Both methods should have detected the presence of {alpha} Cen Bb at a higher significance than is actually seen. We also investigated the influence of random noise with a standard deviation comparable to the HARPS data and sampled in the same way. The distribution of the noise peaks in the period range 2.8-3.3 days has a maximum of Almost-Equal-To 3.2 days and amplitudes approximately one-half of the K-amplitude for the planet. The presence of the activity signal may boost the velocity amplitude of these signals to values comparable to the planet. It may be premature to attribute the 3.24 day RV variations to an Earth-mass planet. A better understanding of the noise characteristics in the RV data as well as more measurements with better sampling will be needed to confirm this exoplanet.

  3. Growth factors in porcine full and partial thickness burn repair. Differing targets and effects of keratinocyte growth factor, platelet-derived growth factor-BB, epidermal growth factor, and neu differentiation factor.

    PubMed Central

    Danilenko, D. M.; Ring, B. D.; Tarpley, J. E.; Morris, B.; Van, G. Y.; Morawiecki, A.; Callahan, W.; Goldenberg, M.; Hershenson, S.; Pierce, G. F.

    1995-01-01

    The topical application of recombinant growth factors such as epidermal growth factor, platelet-derived growth factor-BB homodimer (rPDGF-BB), keratinocyte growth factor (rKGF), and neu differentiation factor has resulted in significant acceleration of healing in several animal models of wound repair. In this study, we established highly reproducible and quantifiable full and deep partial thickness porcine burn models in which burns were escharectomized 4 or 5 days postburn and covered with an occlusive dressing to replicate the standard treatment in human burn patients. We then applied these growth factors to assess their efficacy on several parameters of wound repair: extracellular matrix and granulation tissue production, percent reepithelialization, and new epithelial area. In full thickness burns, only rPDGF-BB and the combination of rPDGF-BB and rKGF induced significant changes in burn repair. rPDGF-BB induced marked extracellular matrix and granulation tissue production (P = 0.013) such that the burn defect was filled within several days of escharectomy, but had no effect on new epithelial area or reepithelialization. The combination of rPDGF-BB and rKGF in full thickness burns resulted in a highly significant increase in extracellular matrix and granulation tissue area (P = 0.0009) and a significant increase in new epithelial area (P = 0.007), but had no effect on reepithelialization. In deep partial thickness burns, rKGF induced the most consistent changes. Daily application of rKGF induced a highly significant increase in new epithelial area (P < 0.0001) but induced only a modest increase in reepithelialization (83.7% rKGF-treated versus 70.2% control; P = 0.016) 12 days postburn. rKGF also doubled the number of fully reepithelialized burns (P = 0.02) at 13 days postburn, at least partially because of marked stimulation of both epidermal and follicular proliferation as assessed by proliferating cell nuclear antigen expression. In situ hybridization for

  4. Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

    PubMed Central

    2012-01-01

    Background There are not reported regarding the protocols for obtaining platelet concentrates (PC) in cats for medical purposes. The objectives of this study were: 1) to describe a manual method for producing two kinds of PC in cats (PC-A and PC-B), 2) to describe the cellular population of the PC, 3) to measure and compare the effect of calcium gluconate (CG) and bovine thrombin (BT) on the temporal release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor type BB (PDGF-BB) at 3 and 12 hours post-activation and 4) to establish correlations between the cellular population of both PCs and the concentration of growth factors (GF). Blood samples were taken from 16 cats for complete blood count, plasma collection and PC preparation. The PC were arbitrarily divided into two fractions, specifically, PC-A (lower fraction) and PC-B (upper fraction). Results The platelet counts were significantly different (P<0.05) between the PC and whole blood but not between the PC fractions. The TGF-β1 concentration efficiencies for PC-A and PC-B activated with CG were 42.86% and 46.54%, and activated with BT were 42.88% and 54.64%, respectively. The PDGF-BB concentration efficiencies for PC-A and PC-B activated with CG were 61.36% and 60.61%, and activated with BT were 65.64% and 72.12%, respectively. The temporal release of GFs showed no statistically significant difference (P>0.05) between the activating substances at the time or for any PC fraction. Conclusions Whatever the activation means, these preparations of cat PC provide significant concentrations of platelets and GFs for possible clinical or experimental use. PMID:23131192

  5. B-B bond activation and NHC ring-expansion reactions of diboron(4) compounds, and accurate molecular structures of B2(NMe2)4, B2eg2, B2neop2 and B2pin2.

    PubMed

    Eck, Martin; Würtemberger-Pietsch, Sabrina; Eichhorn, Antonius; Berthel, Johannes H J; Bertermann, Rüdiger; Paul, Ursula S D; Schneider, Heidi; Friedrich, Alexandra; Kleeberg, Christian; Radius, Udo; Marder, Todd B

    2017-03-14

    In this detailed study we report on the structures of the widely employed diboron(4) compounds bis(pinacolato)diboron (B2pin2) and bis(neopentyl glycolato)diboron (B2neop2), as well as bis(ethylene glycolato)diboron (B2eg2) and tetrakis(dimethylamino)diboron (B2(NMe2)4), and their reactivity, along with that of bis(catecholato)diboron (B2cat2) with backbone saturated and backbone unsaturared N-heterocyclic carbenes (NHCs) of different steric demand. Depending on the nature of the diboron(4) compound and the NHC used, Lewis-acid/Lewis-base adducts or NHC ring-expansion products stemming from B-B and C-N bond activation have been observed. The corresponding NHC adducts and NHC ring-expanded products were isolated and characterised via solid-state and solution NMR spectroscopy and X-ray diffraction. In general, we observed B-B bond and C-N bond activation at low temperature for B2eg2, at room temperature for B2neop2 and at higher temperature for B2cat2. The reactivity strongly depends on steric effects of the NHCs and the diboron(4) compounds, as well as on the corresponding Lewis-basicity and Lewis-acidity. Our results provide profound insight into the chemistry of these diboron(4) reagents with the nowadays ubiquitous NHCs, the stability of the corresponding NHC adducts and on B-B bond activation using Lewis-bases in general. We demonstrate that B-B bond activation may be triggered even at temperatures as low as -40 °C to -30 °C without any metal species involved. For example, the reactions of B2eg2 with sterically less demanding NHCs such as Me2Im(Me) and iPr2Im in solution led to the corresponding ring-expanded products at low temperatures. Furthermore, boronium [L2B(OR)2](+) and borenium [LB(OR)2](+) cations have been observed from the reaction of the bis-borate B2eg3 with the NHCs iPr2Im and Me2Im(Me), which led to the conclusion that the activation of bis-borates with NHCs (or Lewis-bases in general) might be a facile and simple route to access such species.

  6. Search for the Standard Model Higgs boson produced in association with top quarks and decaying into bb¯ in pp collisions at √s = 8 TeV with the ATLAS detector

    SciTech Connect

    Aad, G.

    2015-07-29

    In this study, a search for the Standard Model Higgs boson produced in association with a top-quark pair, tt¯H, is presented. The analysis uses 20.3 fb–1 of pp collision data at √s=8 TeV, collected with the ATLAS detector at the Large Hadron Collider during 2012. The search is designed for the H→bb¯ decay mode and uses events containing one or two electrons or muons. In order to improve the sensitivity of the search, events are categorised according to their jet and b-tagged jet multiplicities. A neural network is used to discriminate between signal and background events, the latter being dominated by tt¯+jets production. In the single-lepton channel, variables calculated using a matrix element method are included as inputs to the neural network to improve discrimination of the irreducible tt¯+bb¯ background. No significant excess of events above the background expectation is found and an observed (expected) limit of 3.4 (2.2) times the Standard Model cross section is obtained at 95 % confidence level. The ratio of the measured tt¯H signal cross section to the Standard Model expectation is found to be μ=1.5±1.1 assuming a Higgs boson mass of 125GeV.

  7. Search for the Standard Model Higgs boson produced in association with top quarks and decaying into bb¯ in pp collisions at √s = 8 TeV with the ATLAS detector

    DOE PAGES

    Aad, G.

    2015-07-29

    In this study, a search for the Standard Model Higgs boson produced in association with a top-quark pair, tt¯H, is presented. The analysis uses 20.3 fb–1 of pp collision data at √s=8 TeV, collected with the ATLAS detector at the Large Hadron Collider during 2012. The search is designed for the H→bb¯ decay mode and uses events containing one or two electrons or muons. In order to improve the sensitivity of the search, events are categorised according to their jet and b-tagged jet multiplicities. A neural network is used to discriminate between signal and background events, the latter being dominatedmore » by tt¯+jets production. In the single-lepton channel, variables calculated using a matrix element method are included as inputs to the neural network to improve discrimination of the irreducible tt¯+bb¯ background. No significant excess of events above the background expectation is found and an observed (expected) limit of 3.4 (2.2) times the Standard Model cross section is obtained at 95 % confidence level. The ratio of the measured tt¯H signal cross section to the Standard Model expectation is found to be μ=1.5±1.1 assuming a Higgs boson mass of 125GeV.« less

  8. The Photospheric Properties of the Underlying White Dwarf in the Nova-like Variable TT ARIETIS during its 1983 Low State

    NASA Astrophysics Data System (ADS)

    Fabian, D.; Nguyen, Q.; Belle, K.; Haung, M.; Sion, E. M.

    1995-12-01

    During 1983, TT Arietis entered a low state of visual magnitude 16 during which accretion declined to an extremely low level and the underlying white dwarf accreter was exposed both in the optical (e.g. the Stark-broadened wings of He II 4686dot {A}) and in the far ultraviolet (Shafter et al. 1985, ApJ, 290, 707). We have analyzed the low resolution IUE SWP spectra of TT Ari obtained during this low state with a grid of high gravity, solar composition model atmospheres convolved with IUE resolution, constructed with TLUSTY and SYNSPEC (Hubeny 1988, Comput.Phys.Comm. 52, 103; Hubeny 1995, TLUSTY and SYNSPEC USERS MANUAL, in press). We present the resulting photospheric parameters of the hot white dwarf, preliminary abundances of its accreted atmosphere, and a quantitative constraint on its global rotational velocity. This work has been supported by NASA LTSA grant NAGW-3726 and by a summer research grant from the NASA Delaware Space Grant Colleges Consortium.

  9. Search for a new resonance decaying to a W or Z boson and a Higgs boson in the ℓℓ/ℓν/νν + bb¯ final states with the ATLAS detector

    SciTech Connect

    Aad, G.

    2015-06-16

    A search for a new resonance decaying to a W or Z boson and a Higgs boson in the ℓℓ/ℓν/νν+bb¯ final states is performed using 20.3 fb-1 of pp collision data recorded at √s = 8 TeV with the ATLAS detector at the Large Hadron Collider. The search is conducted by examining the WH / ZH invariant mass distribution for a localized excess. Thus, no significant deviation from the Standard Model background prediction is observed. The results are interpreted in terms of constraints on the Minimal Walking Technicolor model and on a simplified approach based on a phenomenological Lagrangian of Heavy Vector Triplets.

  10. Search for Higgs Boson pair production in the γγbb¯ final state using pp collision data at s=8TeV from the ATLAS Detector

    DOE PAGES

    Aad, G.; Abbott, B.; Abdallah, J.; ...

    2015-02-26

    Searches are performed for resonant and nonresonant Higgs boson pair production in the γγbb¯ final state using 20  fb⁻¹ of proton-proton collisions at a center-of-mass energy of 8 TeV recorded with the ATLAS detector at the CERN Large Hadron Collider. A 95% confidence level upper limit on the cross section times branching ratio of nonresonant production is set at 2.2 pb, while the expected limit is 1.0 pb. The difference derives from a modest excess of events, corresponding to 2.4 standard deviations from the background-only hypothesis. The limit observed in the search for a narrow X → hh resonance ranges betweenmore » 0.7 and 3.5 pb as a function of the resonance mass.« less

  11. Search for Higgs boson pair production in the γγbb[over ¯] final state using pp collision data at sqrt[s]=8  TeV from the ATLAS detector.

    PubMed

    Aad, G; Abbott, B; Abdallah, J; Abdel Khalek, S; Abdinov, O; Aben, R; Abi, B; Abolins, M; AbouZeid, O S; Abramowicz, H; Abreu, H; Abreu, R; Abulaiti, Y; Acharya, B S; Adamczyk, L; Adams, D L; Adelman, J; Adomeit, S; Adye, T; Agatonovic-Jovin, T; Aguilar-Saavedra, J A; Agustoni, M; Ahlen, S P; Ahmadov, F; Aielli, G; Akerstedt, H; Åkesson, T P A; Akimoto, G; Akimov, A V; Alberghi, G L; Albert, J; Albrand, S; Alconada Verzini, M J; Aleksa, M; Aleksandrov, I N; Alexa, C; Alexander, G; Alexandre, G; Alexopoulos, T; Alhroob, M; Alimonti, G; Alio, L; Alison, J; Allbrooke, B M M; Allison, L J; Allport, P P; Almond, J; Aloisio, A; Alonso, A; Alonso, F; Alpigiani, C; Altheimer, A; Alvarez Gonzalez, B; Alviggi, M G; Amako, K; Amaral Coutinho, Y; Amelung, C; Amidei, D; Amor Dos Santos, S P; Amorim, A; Amoroso, S; Amram, N; Amundsen, G; Anastopoulos, C; Ancu, L S; Andari, N; Andeen, T; Anders, C F; Anders, G; Anderson, K J; Andreazza, A; Andrei, V; Anduaga, X S; Angelidakis, S; Angelozzi, I; Anger, P; Angerami, A; Anghinolfi, F; Anisenkov, A V; Anjos, N; Annovi, A; Antonaki, A; Antonelli, M; Antonov, A; Antos, J; Anulli, F; Aoki, M; Aperio Bella, L; Apolle, R; Arabidze, G; Aracena, I; Arai, Y; Araque, J P; Arce, A T H; Arguin, J-F; Argyropoulos, S; Arik, M; Armbruster, A J; Arnaez, O; Arnal, V; Arnold, H; Arratia, M; Arslan, O; Artamonov, A; Artoni, G; Asai, S; Asbah, N; Ashkenazi, A; Åsman, B; Asquith, L; Assamagan, K; Astalos, R; Atkinson, M; Atlay, N B; Auerbach, B; Augsten, K; Aurousseau, M; Avolio, G; Azuelos, G; Azuma, Y; Baak, M A; Baas, A; Bacci, C; Bachacou, H; Bachas, K; Backes, M; Backhaus, M; Backus Mayes, J; Badescu, E; Bagiacchi, P; Bagnaia, P; Bai, Y; Bain, T; Baines, J T; Baker, O K; Balek, P; Balli, F; Banas, E; Banerjee, Sw; Bannoura, A A E; Bansal, V; Bansil, H S; Barak, L; Baranov, S P; Barberio, E L; Barberis, D; Barbero, M; Barillari, T; Barisonzi, M; Barklow, T; Barlow, N; Barnett, B M; Barnett, R M; Barnovska, Z; Baroncelli, A; Barone, G; Barr, A J; Barreiro, F; Barreiro Guimarães da Costa, J; Bartoldus, R; Barton, A E; Bartos, P; Bartsch, V; Bassalat, A; Basye, A; Bates, R L; Batkova, L; Batley, J R; Battaglia, M; Battistin, M; Bauer, F; Bawa, H S; Beau, T; Beauchemin, P H; Beccherle, R; Bechtle, P; Beck, H P; Becker, K; Becker, S; Beckingham, M; Becot, C; Beddall, A J; Beddall, A; Bedikian, S; Bednyakov, V A; Bee, C P; Beemster, L J; Beermann, T A; Begel, M; Behr, K; Belanger-Champagne, C; Bell, P J; Bell, W H; Bella, G; Bellagamba, L; Bellerive, A; Bellomo, M; Belotskiy, K; Beltramello, O; Benary, O; Benchekroun, D; Bendtz, K; Benekos, N; Benhammou, Y; Benhar Noccioli, E; Benitez Garcia, J A; Benjamin, D P; Bensinger, J R; Benslama, K; Bentvelsen, S; Berge, D; Bergeaas Kuutmann, E; Berger, N; Berghaus, F; Beringer, J; Bernard, C; Bernat, P; Bernius, C; Bernlochner, F U; Berry, T; Berta, P; Bertella, C; Bertoli, G; Bertolucci, F; Bertsche, D; Besana, M I; Besjes, G J; Bessidskaia Bylund, O; Bessner, M F; Besson, N; Betancourt, C; Bethke, S; Bhimji, W; Bianchi, R M; Bianchini, L; Bianco, M; Biebel, O; Bieniek, S P; Bierwagen, K; Biesiada, J; Biglietti, M; Bilbao De Mendizabal, J; Bilokon, H; Bindi, M; Binet, S; Bingul, A; Bini, C; Black, C W; Black, J E; Black, K M; Blackburn, D; Blair, R E; Blanchard, J-B; Blazek, T; Bloch, I; Blocker, C; Blum, W; Blumenschein, U; Bobbink, G J; Bobrovnikov, V S; Bocchetta, S S; Bocci, A; Bock, C; Boddy, C R; Boehler, M; Boek, T T; Bogaerts, J A; Bogdanchikov, A G; Bogouch, A; Bohm, C; Bohm, J; Boisvert, V; Bold, T; Boldea, V; Boldyrev, A S; Bomben, M; Bona, M; Boonekamp, M; Borisov, A; Borissov, G; Borri, M; Borroni, S; Bortfeldt, J; Bortolotto, V; Bos, K; Boscherini, D; Bosman, M; Boterenbrood, H; Boudreau, J; Bouffard, J; Bouhova-Thacker, E V; Boumediene, D; Bourdarios, C; Bousson, N; Boutouil, S; Boveia, A; Boyd, J; Boyko, I R; Bracinik, J; Brandt, A; Brandt, G; Brandt, O; Bratzler, U; Brau, B; Brau, J E; Braun, H M; Brazzale, S F; Brelier, B; Brendlinger, K; Brennan, A J; Brenner, R; Bressler, S; Bristow, K; Bristow, T M; Britton, D; Brochu, F M; Brock, I; Brock, R; Bromberg, C; Bronner, J; Brooijmans, G; Brooks, T; Brooks, W K; Brosamer, J; Brost, E; Brown, J; Bruckman de Renstrom, P A; Bruncko, D; Bruneliere, R; Brunet, S; Bruni, A; Bruni, G; Bruschi, M; Bryngemark, L; Buanes, T; Buat, Q; Bucci, F; Buchholz, P; Buckingham, R M; Buckley, A G; Buda, S I; Budagov, I A; Buehrer, F; Bugge, L; Bugge, M K; Bulekov, O; Bundock, A C; Burckhart, H; Burdin, S; Burghgrave, B; Burke, S; Burmeister, I; Busato, E; Büscher, D; Büscher, V; Bussey, P; Buszello, C P; Butler, B; Butler, J M; Butt, A I; Buttar, C M; Butterworth, J M; Butti, P; Buttinger, W; Buzatu, A; Byszewski, M; Cabrera Urbán, S; Caforio, D; Cakir, O; Calafiura, P; Calandri, A; Calderini, G; Calfayan, P; Calkins, R; Caloba, L P; Calvet, D; Calvet, S; Camacho Toro, R; Camarda, S; Cameron, D; Caminada, L M; Caminal Armadans, R; Campana, S; Campanelli, M; Campoverde, A; Canale, V; Canepa, A; Cano Bret, M; Cantero, J; Cantrill, R; Cao, T; Capeans Garrido, M D M; Caprini, I; Caprini, M; Capua, M; Caputo, R; Cardarelli, R; Carli, T; Carlino, G; Carminati, L; Caron, S; Carquin, E; Carrillo-Montoya, G D; Carter, J R; Carvalho, J; Casadei, D; Casado, M P; Casolino, M; Castaneda-Miranda, E; Castelli, A; Castillo Gimenez, V; Castro, N F; Catastini, P; Catinaccio, A; Catmore, J R; Cattai, A; Cattani, G; Caughron, S; Cavaliere, V; Cavalli, D; Cavalli-Sforza, M; Cavasinni, V; Ceradini, F; Cerio, B; Cerny, K; Cerqueira, A S; Cerri, A; Cerrito, L; Cerutti, F; Cerv, M; Cervelli, A; Cetin, S A; Chafaq, A; Chakraborty, D; Chalupkova, I; Chang, P; Chapleau, B; Chapman, J D; Charfeddine, D; Charlton, D G; Chau, C C; Chavez Barajas, C A; Cheatham, S; Chegwidden, A; Chekanov, S; Chekulaev, S V; Chelkov, G A; Chelstowska, M A; Chen, C; Chen, H; Chen, K; Chen, L; Chen, S; Chen, X; Chen, Y; Cheng, H C; Cheng, Y; Cheplakov, A; Cherkaoui El Moursli, R; Chernyatin, V; Cheu, E; Chevalier, L; Chiarella, V; Chiefari, G; Childers, J T; Chilingarov, A; Chiodini, G; Chisholm, A S; Chislett, R T; Chitan, A; Chizhov, M V; Chouridou, S; Chow, B K B; Chromek-Burckhart, D; Chu, M L; Chudoba, J; Chwastowski, J J; Chytka, L; Ciapetti, G; Ciftci, A K; Ciftci, R; Cinca, D; Cindro, V; Ciocio, A; Cirkovic, P; Citron, Z H; Citterio, M; Ciubancan, M; Clark, A; Clark, P J; Clarke, R N; Cleland, W; Clemens, J C; Clement, C; Coadou, Y; Cobal, M; Coccaro, A; Cochran, J; Coffey, L; Cogan, J G; Coggeshall, J; Cole, B; Cole, S; Colijn, A P; Collot, J; Colombo, T; Colon, G; Compostella, G; Conde Muiño, P; Coniavitis, E; Conidi, M C; Connell, S H; Connelly, I A; Consonni, S M; Consorti, V; Constantinescu, S; Conta, C; Conti, G; Conventi, F; Cooke, M; Cooper, B D; Cooper-Sarkar, A M; Cooper-Smith, N J; Copic, K; Cornelissen, T; Corradi, M; Corriveau, F; Corso-Radu, A; Cortes-Gonzalez, A; Cortiana, G; Costa, G; Costa, M J; Costanzo, D; Côté, D; Cottin, G; Cowan, G; Cox, B E; Cranmer, K; Cree, G; Crépé-Renaudin, S; Crescioli, F; Cribbs, W A; Crispin Ortuzar, M; 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    2015-02-27

    Searches are performed for resonant and nonresonant Higgs boson pair production in the γγbb[over ¯] final state using 20  fb^{-1} of proton-proton collisions at a center-of-mass energy of 8 TeV recorded with the ATLAS detector at the CERN Large Hadron Collider. A 95% confidence level upper limit on the cross section times branching ratio of nonresonant production is set at 2.2 pb, while the expected limit is 1.0 pb. The difference derives from a modest excess of events, corresponding to 2.4 standard deviations from the background-only hypothesis. The limit observed in the search for a narrow X→hh resonance ranges between 0.7 and 3.5 pb as a function of the resonance mass.

  12. Search for the standard model Higgs boson decaying to a bb pair in events with one charged lepton and large missing transverse energy using the full CDF data set.

    PubMed

    Aaltonen, T; Álvarez González, B; Amerio, S; Amidei, D; Anastassov, A; Annovi, A; Antos, J; Apollinari, G; Appel, J A; Arisawa, T; Artikov, A; Asaadi, J; Ashmanskas, W; Auerbach, B; Aurisano, A; Azfar, F; Badgett, W; Bae, T; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Barria, P; Bartos, P; Bauce, M; Bedeschi, F; Behari, S; Bellettini, G; Bellinger, J; Benjamin, D; Beretvas, A; Bhatti, A; Binkley, M E; Bisello, D; Bizjak, I; Bland, K R; Blumenfeld, B; Bocci, A; Bodek, A; Bortoletto, D; Boudreau, J; Boveia, A; Brigliadori, L; Bromberg, C; Brucken, E; Budagov, J; Budd, H S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Calamba, A; Calancha, C; Camarda, S; Campanelli, M; Campbell, M; Canelli, F; Carls, B; Carlsmith, D; Carosi, R; Carrillo, S; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavaliere, V; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, K; Chokheli, D; Chung, W H; Chung, Y S; Ciocci, M A; Clark, A; Clarke, C; Compostella, G; Convery, M E; Conway, J; Corbo, M; Cordelli, M; Cox, C A; Cox, D J; Crescioli, F; Cuevas, J; Culbertson, R; Dagenhart, D; d'Ascenzo, N; Datta, M; de Barbaro, P; Dell'Orso, M; Demortier, L; Deninno, M; Devoto, F; d'Errico, M; Di Canto, A; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Donati, S; Dong, P; Dorigo, M; Dorigo, T; Ebina, K; Elagin, A; Eppig, A; Erbacher, R; Errede, S; Ershaidat, N; Eusebi, R; Farrington, S; Feindt, M; Fernandez, J P; Ferrazza, C; Field, R; Flanagan, G; Forrest, R; Frank, M J; Franklin, M; Freeman, J C; Funakoshi, Y; Furic, I; Gallinaro, M; Garcia, J E; Garfinkel, A F; Garosi, P; Gerberich, H; Gerchtein, E; Giagu, S; Giakoumopoulou, V; Giannetti, P; Gibson, K; Ginsburg, C M; Giokaris, N; Giromini, P; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldin, D; Goldschmidt, N; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Grinstein, S; Grosso-Pilcher, C; Group, R C; Guimaraes da Costa, J; Hahn, S R; Halkiadakis, E; Hamaguchi, A; Han, J Y; Happacher, F; Hara, K; Hare, D; Hare, M; Harr, R F; Hatakeyama, K; Hays, C; Heck, M; Heinrich, J; Herndon, M; Hewamanage, S; Hocker, A; Hopkins, W; Horn, D; Hou, S; Hughes, R E; Hurwitz, M; Husemann, U; Hussain, N; Hussein, M; Huston, J; Introzzi, G; Iori, M; Ivanov, A; James, E; Jang, D; Jayatilaka, B; Jeans, D T; Jeon, E J; Jindariani, S; Jones, M; Joo, K K; Jun, S Y; Junk, T R; Kamon, T; Karchin, P E; Kasmi, A; Kato, Y; Ketchum, W; Keung, J; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kim, Y J; Kimura, N; Kirby, M; Klimenko, S; Knoepfel, K; Kondo, K; Kong, D J; Konigsberg, J; Kotwal, A V; Kreps, M; Kroll, J; Krop, D; Kruse, M; Krutelyov, V; Kuhr, T; Kurata, M; Kwang, S; Laasanen, A T; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; LeCompte, T; Lee, E; Lee, H S; Lee, J S; Lee, S W; Leo, S; Leone, S; Lewis, J D; Limosani, A; Lin, C-J; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, C; Liu, H; Liu, Q; Liu, T; Lockwitz, S; Loginov, A; Lucchesi, D; Lueck, J; Lujan, P; Lukens, P; Lungu, G; Lys, J; Lysak, R; Madrak, R; Maeshima, K; Maestro, P; Malik, S; Manca, G; Manousakis-Katsikakis, A; Margaroli, F; Marino, C; Martínez, M; Mastrandrea, P; Matera, K; Mattson, M E; Mazzacane, A; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Mesropian, C; Miao, T; Mietlicki, D; Mitra, A; Miyake, H; Moed, S; Moggi, N; Mondragon, M N; Moon, C S; Moore, R; Morello, M J; Morlock, J; Movilla Fernandez, P; Mukherjee, A; Muller, Th; Murat, P; Mussini, M; Nachtman, J; Nagai, Y; Naganoma, J; Nakano, I; Napier, A; Nett, J; Neu, C; Neubauer, M S; Nielsen, J; Nodulman, L; Noh, S Y; Norniella, O; Oakes, L; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Orava, R; Ortolan, L; Pagan Griso, S; Pagliarone, C; Palencia, E; Papadimitriou, V; Paramonov, A A; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Pianori, E; Pilot, J; Pitts, K; Plager, C; Pondrom, L; Poprocki, S; Potamianos, K; Prokoshin, F; Pranko, A; Ptohos, F; Punzi, G; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Renton, P; Rescigno, M; Riddick, T; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rodriguez, T; Rogers, E; Rolli, S; Roser, R; Ruffini, F; Ruiz, A; Russ, J; Rusu, V; Safonov, A; Sakumoto, W K; Sakurai, Y; Santi, L; Sato, K; Saveliev, V; Savoy-Navarro, A; Schlabach, P; Schmidt, A; Schmidt, E E; Schwarz, T; Scodellaro, L; Scribano, A; Scuri, F; Seidel, S; Seiya, Y; Semenov, A; Sforza, F; Shalhout, S Z; Shears, T; Shepard, P F; Shimojima, M; Shochet, M; Shreyber-Tecker, I; Simonenko, A; Sinervo, P; Sliwa, K; Smith, J R; Snider, F D; Soha, A; Sorin, V; Song, H; Squillacioti, P; Stancari, M; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Strycker, G L; Sudo, Y; Sukhanov, A; Suslov, I; Takemasa, K; Takeuchi, Y; Tang, J; Tecchio, M; Teng, P K; Thom, J; Thome, J; Thompson, G A; Thomson, E; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Totaro, P; Trovato, M; Ukegawa, F; Uozumi, S; Varganov, A; Vázquez, F; Velev, G; Vellidis, C; Vidal, M; Vila, I; Vilar, R; Vizán, J; Vogel, M; Volpi, G; Wagner, P; Wagner, R L; Wakisaka, T; Wallny, R; Wang, S M; Warburton, A; Waters, D; Wester, W C; Whiteson, D; Wicklund, A B; Wicklund, E; Wilbur, S; Wick, F; Williams, H H; Wilson, J S; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, H; Wright, T; Wu, X; Wu, Z; Yamamoto, K; Yamato, D; Yang, T; Yang, U K; Yang, Y C; Yao, W-M; Yeh, G P; Yi, K; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanetti, A; Zeng, Y; Zhou, C; Zucchelli, S

    2012-09-14

    We present a search for the standard model Higgs boson produced in association with a W boson in sqrt[s]=1.96  TeV pp collision data collected with the CDF II detector at the Tevatron corresponding to an integrated luminosity of 9.45  fb(-1). In events consistent with the decay of the Higgs boson to a bottom-quark pair and the W boson to an electron or muon and a neutrino, we set 95% credibility level upper limits on the WH production cross section times the H→bb branching ratio as a function of Higgs boson mass. At a Higgs boson mass of 125  GeV/c(2), we observe (expect) a limit of 4.9 (2.8) times the standard model value.

  13. Search for a new resonance decaying to a W or Z boson and a Higgs boson in the ℓℓ/ℓν/νν + bb¯ final states with the ATLAS detector

    DOE PAGES

    Aad, G.

    2015-06-16

    A search for a new resonance decaying to a W or Z boson and a Higgs boson in the ℓℓ/ℓν/νν+bb¯ final states is performed using 20.3 fb-1 of pp collision data recorded at √s = 8 TeV with the ATLAS detector at the Large Hadron Collider. The search is conducted by examining the WH / ZH invariant mass distribution for a localized excess. Thus, no significant deviation from the Standard Model background prediction is observed. The results are interpreted in terms of constraints on the Minimal Walking Technicolor model and on a simplified approach based on a phenomenological Lagrangian ofmore » Heavy Vector Triplets.« less

  14. Evaluation of corn hybrids expressing Cry1F, cry1A.105, Cry2Ab2, Cry34Ab1/Cry35Ab1, and Cry3Bb1 against southern United States insect pests.

    PubMed

    Siebert, M W; Nolting, S P; Hendrix, W; Dhavala, S; Craig, C; Leonard, B R; Stewart, S D; All, J; Musser, F R; Buntin, G D; Samuel, L

    2012-10-01

    Studies were conducted across the southern United States to characterize the efficacy of multiple Bacillus thuringiensis (Bt) events in a field corn, Zea mays L., hybrid for control of common lepidopteran and coleopteran pests. Cry1F protein in event TC1507 and Cry1A.105 + Cry2Ab2 proteins in event MON 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. Cry34Ab1/Cry35Ab1 proteins in event DAS-59122-7 and Cry3Bb1 in event MON 88017 were evaluated against the larvae of Mexican corn rootworm, Diabrotica virgifera zeae Krysan and Smith, which occur below-ground. Field corn hybrids containing Cry1F, Cry1A.105 + Cry2Ab2, Cry34Ab1/Cry35Ab1, and Cry3Bb1 insecticidal proteins (SmartStax) consistently demonstrated reductions in plant injury and/or reduced larval survivorship as compared with a non-Bt field corn hybrid. Efficacy provided by a field corn hybrid with multiple Bt proteins was statistically equal to or significantly better than corn hybrids containing a single event active against target pests. Single event field corn hybrids provided very high levels of control of southwestern corn borer, Diatraea grandiosella (Dyar), lesser cornstalk borer, Elasmopalpus lignosellus (Zeller), and fall armyworm, Spodoptera frugiperda (J.E. Smith), and were not significantly different than field corn hybrids with multiple events. Significant increases in efficacy were observed for a field corn hybrid with multiple Bt events for sugarcane borer, Diatraea saccharalis (F.), beet armyworm, Spodoptera exigua (Hübner), corn earworm, Helicoverpa zea (Boddie), and Mexican corn rootworm. Utilization of field corn hybrids containing multiple Bt events provides a means for managing insect resistance to Bt proteins and reduces non-Bt corn refuge requirements.

  15. A comprehensive proteomics and genomics analysis reveals novel transmembrane proteins in human platelets and mouse megakaryocytes including G6b-B, a novel immunoreceptor tyrosine-based inhibitory motif protein.

    PubMed

    Senis, Yotis A; Tomlinson, Michael G; García, Angel; Dumon, Stephanie; Heath, Victoria L; Herbert, John; Cobbold, Stephen P; Spalton, Jennifer C; Ayman, Sinem; Antrobus, Robin; Zitzmann, Nicole; Bicknell, Roy; Frampton, Jon; Authi, Kalwant S; Martin, Ashley; Wakelam, Michael J O; Watson, Stephen P

    2007-03-01

    The platelet surface is poorly characterized due to the low abundance of many membrane proteins and the lack of specialist tools for their investigation. In this study we identified novel human platelet and mouse megakaryocyte membrane proteins using specialist proteomics and genomics approaches. Three separate methods were used to enrich platelet surface proteins prior to identification by liquid chromatography and tandem mass spectrometry: lectin affinity chromatography, biotin/NeutrAvidin affinity chromatography, and free flow electrophoresis. Many known, abundant platelet surface transmembrane proteins and several novel proteins were identified using each receptor enrichment strategy. In total, two or more unique peptides were identified for 46, 68, and 22 surface membrane, intracellular membrane, and membrane proteins of unknown subcellular localization, respectively. The majority of these were single transmembrane proteins. To complement the proteomics studies, we analyzed the transcriptome of a highly purified preparation of mature primary mouse megakaryocytes using serial analysis of gene expression in view of the increasing importance of mutant mouse models in establishing protein function in platelets. This approach identified all of the major classes of platelet transmembrane receptors, including multitransmembrane proteins. Strikingly 17 of the 25 most megakaryocyte-specific genes (relative to 30 other serial analysis of gene expression libraries) were transmembrane proteins, illustrating the unique nature of the megakaryocyte/platelet surface. The list of novel plasma membrane proteins identified using proteomics includes the immunoglobulin superfamily member G6b, which undergoes extensive alternate splicing. Specific antibodies were used to demonstrate expression of the G6b-B isoform, which contains an immunoreceptor tyrosine-based inhibition motif. G6b-B undergoes tyrosine phosphorylation and association with the SH2 domain-containing phosphatase

  16. Antiproliferation effect of imatinib mesylate on MCF7, T-47D tumorigenic and MCF 10A nontumorigenic breast cell lines via PDGFR-β, PDGF-BB, c-Kit and SCF genes

    PubMed Central

    Kadivar, Ali; Kamalidehghan, Behnam; Akbari Javar, Hamid; Karimi, Benyamin; Sedghi, Reihaneh; Noordin, Mohamed Ibrahim

    2017-01-01

    Recent cancer molecular therapies are targeting main functional molecules to control applicable process of cancer cells. Attractive targets are established by receptor tyrosine kinases, such as platelet-derived growth factor receptors (PDGFRs) and c-Kit as mostly irregular signaling, which is due to either over expression or mutation that is associated with tumorigenesis and cell proliferation. Imatinib mesylate is a selective inhibitor of receptor tyrosine kinase, including PDGFR-β and c-Kit. In this research, we studied how imatinib mesylate would exert effect on MCF7 and T-47D breast cancer and MCF 10A epithelial cell lines, the gene and protein expression of PDGFR-β, c-Kit and their relevant ligands platelet-derived growth factor (PDGF)-BB and stem cell factor (SCF). The MTS assay was conducted in therapeutic relevant concentration of 2–10 µM for 96, 120 and 144 h treatment. In addition, apoptosis induction and cytostatic activity of imatinib mesylate were investigated with the terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL and cell cycle assays, respectively, in a time-dependent manner. Comparative real-time PCR and Western blot analysis were conducted to evaluate the expression and regulation of imatinib target genes and proteins. Our finding revealed that imatinib mesylate antiproliferation effect, apoptosis induction and cytostatic activity were significantly higher in breast cancer cell lines compared to MCF 10A. This effect might be due to the expression of PDGFR-β, PDGF-BB, c-Kit and SCF, which was expressed by all examined cell lines, except the T-47D cell line which was not expressed c-Kit. However, examined gene and proteins expressed more in cancer cell lines. Therefore, imatinib mesylate was more effective on them. It is concluded that imatinib has at least two potential targets in both examined breast cancer cell lines and can be a promising drug for targeted therapy to treat breast cancer. PMID:28260860

  17. Occurrence of selected polybrominated diphenyl ethers and 2,2',4,4',5,5'-hexabromobiphenyl (BB-153) in sewage sludge and effluent samples of a wastewater-treatment plant in Cape Town, South Africa.

    PubMed

    Daso, Adegbenro P; Fatoki, Olalekan S; Odendaal, James P; Olujimi, Olanrewaju O

    2012-04-01

    The reuse of treated effluent from wastewater treatment plants (WWTPs) as alternative water source for sport-field or landscape irrigation, agricultural, and other industrial purposes is growing significantly. Similarly, the application of treated sludge (biosolid) to agricultural soils is now being considered globally as the most economic means of sludge disposal. However, the presence of emerging organic contaminants in these matrices, including polybrominated diphenyl ethers (PBDEs), which are potential endocrine disruptors, portends a high health risk to humans and the environment in general. In this study, effluent and sewage sludge samples collected from a WWTP were analysed for some selected PBDE congeners (BDE congeners 28, 47, 99 100 153 154 183, and 209) as well as BB-153 using a high-capillary gas chromatograph equipped with an electron capture detector. The sum of the eight PBDE congeners ranged from 369 to 4370, 19.2 to 2640, and 90.4 to 15,100 ng/l for raw water, secondary effluent, and final effluent, respectively. A similar result was observed for sewage sludge samples, which ranged between 13.1 and 652 ng/g dry weight (dw). The results obtained for BB-153 were generally lower compared with those found for most PBDE congeners. These ranged from ND to 18.4 ng/l and ND to 9.97 ng/g dw for effluents and sewage sludge, respectively. In both matrices, BDE 47 and 209 congeners were found to contribute significantly to the overall sum of PBDEs. The reuse of the treated effluent, particularly for agricultural purposes, could enhance the possibility of these contaminants entering into the food chain, thus causing undesirable health problems in exposed subjects.

  18. THE SPECTROSCOPIC ORBITS OF FIVE γ DORADUS STARS

    SciTech Connect

    Fekel, Francis C.; Henry, Gregory W.; Pourbaix, Dimitri E-mail: gregory.w.henry@gmail.com

    2016-02-15

    We have determined the spectroscopic orbits of five γ Dor variables, HD 776, HD 6568, HD 17310, HD 19684, and HD 62196. Their orbital periods range from 27.8 to 1163 days and their eccentricities from 0.01 to 0.65. Of the five systems, only HD 19684 shows lines of its binary companion, but those lines are always so weak and blended with the lines of the primary that we were unable to measure them satisfactorily. The velocity residuals of the orbital fits were searched for periodicities associated with pulsation. No clear, convincing case for velocity periodicities in the residuals was found in four of the five stars. However, for HD 17310 we identified a period of 2.13434 days, a value in agreement with the largest amplitude period previously found photometrically for that star. The velocity residuals of HD 62196 have a long-term trend suggesting that it is a triple system.

  19. The Spectroscopic Orbits of Five γ Doradus Stars

    NASA Astrophysics Data System (ADS)

    Fekel, Francis C.; Henry, Gregory W.; Pourbaix, Dimitri

    2016-02-01

    We have determined the spectroscopic orbits of five γ Dor variables, HD 776, HD 6568, HD 17310, HD 19684, and HD 62196. Their orbital periods range from 27.8 to 1163 days and their eccentricities from 0.01 to 0.65. Of the five systems, only HD 19684 shows lines of its binary companion, but those lines are always so weak and blended with the lines of the primary that we were unable to measure them satisfactorily. The velocity residuals of the orbital fits were searched for periodicities associated with pulsation. No clear, convincing case for velocity periodicities in the residuals was found in four of the five stars. However, for HD 17310 we identified a period of 2.13434 days, a value in agreement with the largest amplitude period previously found photometrically for that star. The velocity residuals of HD 62196 have a long-term trend suggesting that it is a triple system.

  20. γ Doradus Pulsations in the Eclipsing Binary Star KIC 6048106

    NASA Astrophysics Data System (ADS)

    Lee, Jae Woo

    2016-12-01

    We present the Kepler photometry of KIC 6048106, which is exhibiting the O’Connell effect and multiperiodic pulsations. Including a starspot on either of the components, light-curve synthesis indicates that this system is a semi-detached Algol with a mass ratio of 0.211, an orbital inclination of 73.°9, and a large temperature difference of 2534 K. To examine in detail both the spot variations and pulsations, we separately analyzed the Kepler time-series data at the interval of an orbital period in an iterative way. The results reveal that the variable asymmetries of the light maxima can be interpreted as the changes with time of a magnetic cool spot on the secondary component. Multiple frequency analyses were performed in the outside-eclipse light residuals after removal of the binarity effects from the observed Kepler data. We detected 30 frequencies with signal to noise amplitude ratios larger than 4.0, of which six (f 2-f 6 and f 10) can be identified as high-order (17 ≤ n ≤ 25) low-degree (ℓ = 2) gravity-mode pulsations that were stable during the observing run of 200 days. In contrast, the other frequencies may be harmonic and combination terms. For the six frequencies, the pulsation periods and pulsation constants are in the ranges of 0.352-0.506 days and 0.232-0.333 days, respectively. These values and the position on the Hertzsprung-Russell diagram demonstrate that the primary star is a γ Dor variable. The evolutionary status and the pulsation nature of KIC 6048106 are discussed.

  1. Monoclonal antibody specific to a subclass of polyproline-Arg motif provides evidence for the presence of an snRNA-free spliceosomal Sm protein complex in vivo: implications for molecular interactions involving proline-rich sequences of Sm B/B' proteins.

    PubMed

    Filali, M; Qiu, J; Awasthi, S; Fischer, U; Monos, D; Kamoun, M

    1999-08-01

    The human spliceosomal Sm B/B' proteins are essential for the biogenesis of the snRNP particles. B/B' proteins contain several clusters of the PPPPGM/IR sequence, which occurs within the C-terminus of Sm B/B'. This sequence is very similar to the PPPPPGHR sequence of the cytoplasmic tail of the CD2 receptor and closely resembles the class II of SH3 ligands, suggesting a similarly important role. We report that a monoclonal antibody (3E10) against the PPPPPGHR sequence recognizes spliceosomal Sm B/B' proteins. Proteins that are specifically immunoprecipitated by 3E10 include Sm B, B', D1, D2, D3, E, F, and G. However, unlike Y12 and other anti-Sm immunoprecipitates, 3E10 immunoprecipitates appear to lack the U1 snRNP-specific proteins A and C and U snRNAs. These findings indicate that 3E10 recognizes a subset of Sm protein core and suggest the presence of snRNA-free Sm protein complex(es) in vivo. We propose that the epitope binding for 3E10 may become unaccessible upon interactions of Sm proteins and their subsequent incorporation into the core particles. The Sm proline-rich sequences may have an important role in mediating protein-protein interactions necessary for the proper snRNP core assembly or function, or both. To our knowledge, 3E10 is the first well characterized mAb specific for a subclass of polyproline-arg motif recognizing Sm B/B' and CD2 proteins. 3E10 antibody can be used to further characterize the nature of protein components in the snRNA-free Sm subcore protein complex(es) that are formed during the snRNP core assembly steps.

  2. Using new non-invasive quick method to detect Borrelia Burgdorferi (B.B.) infection from specific parts of the heart in "seemingly normal" ECGs, and from the ECGs of Atrial Fibrillation (AF), a majority of AF ECGs are found to have: 1) Significant B.B. infection, 2) Markedly increased ANP, 3) Increased Cardiac Troponin I & 4) Markedly reduced Taurine. These 4 factors were mainly localized at infected areas of the SA node area, R-&L-Atria & pulmonary veins at the L-atrium.

    PubMed

    Omura, Yoshiaki; Lu, Dominic; Jones, Marilyn K; Nihrane, Abdallah; Duvvi, Harsha; Yapor, Dario; Shimotsuura, Yasuhiro; Ohki, Motomu

    2015-01-01

    Lyme disease is found in a majority of people we tested. Once Borrelia Burgdorferi (B.B.) spirochete enters human body, it not only causes pain by infecting joints, but it also often enters the brain and the heart. Infection of brain can be quickly detected from the pupil and infection of the heart by ECGs non-invasively. By evaluating recorded ECGs of atrial fibrillation (AF), using U.S. patented non-invasive highly sensitive electromagnetic field (EMF) resonance phenomenon between 2 identical molecules or between a molecule and its antibody, we examined 25 different AF patients' ECGs and found the majority of them suffer from various degrees of B.B. spirochete infection in SA node areas, also in the right & left atria, and pulmonary vein near and around its junction at left atrium & lesser degrees of infection at the AV node & His Bundle. When B.B. infection reaches over 224-600ng or higher at these areas, AF often appears in the majority of all AF analyzed. In order to develop AF, the 4 abnormal factors must be present simultaneously: 1) B.B. infection must be increased to 224-600ng or higher, 2) Atrial Natriuretic Peptide (ANP) must be markedly reduced from normal value of less than 4ng to over 100-400ng, 3) A significant increase of Cardiac Troponin I from normal value of less than 3ng to over 12ng and 4) Taurine must also be markedly reduced from normal value of 4-6ng to 0.25ng. These 4 changes were mainly found only at infected sites of the SA node area, both atria and between the end of the T wave & the beginning of the SA node area, which corresponds to U waves at recorded ECG. Origin of the U wave is mainly due to abnormal electrical potential of pulmonary vein at L-atrium. If all 4 factors do not occur at the infection site, no AF will develop. In seemingly normal ECGs, if using this method, one can detect invisible B.B. infection in early stages. Long before AF appears, AF can be prevented by improved treatment with Amoxicillin 500ng 3 times

  3. Typical values of the electric drift E × B/B2 in the inner radiation belt and slot region as determined from Van Allen Probe measurements

    NASA Astrophysics Data System (ADS)

    Lejosne, Solène; Mozer, F. S.

    2016-12-01

    The electric drift E × B/B2 plays a fundamental role for the description of plasma flow and particle acceleration. Yet it is not well-known in the inner belt and slot region because of a lack of reliable in situ measurements. In this article, we present an analysis of the electric drifts measured below L 3 by both Van Allen Probes A and B from September 2012 to December 2014. The objective is to determine the typical components of the equatorial electric drift in both radial and azimuthal directions. The dependences of the components on radial distance, magnetic local time, and geographic longitude are examined. The results from Van Allen Probe A agree with Van Allen Probe B. They show, among other things, a typical corotation lag of the order of 5 to 10% below L 2.6, as well as a slight radial transport of the order of 20 m s-1. The magnetic local time dependence of the electric drift is consistent with that of the ionosphere wind dynamo below L 2 and with that of a solar wind-driven convection electric field above L 2. A secondary longitudinal dependence of the electric field is also found. Therefore, this work also demonstrates that the instruments on board Van Allen Probes are able to perform accurate measurements of the electric drift below L 3.

  4. Aging affect the anti-tumor potential of dendritic cell vaccination, but it can be overcome by co-stimulation with anti-OX40 or anti-4-1BB.

    PubMed

    Sharma, Sanjay; Dominguez, Ana Lucia; Lustgarten, Joseph

    2006-01-01

    It has been well established that there is a decline in immune function with age resulting in a diminished capacity to respond to infections or tumors. Although many studies have demonstrated the efficacy of autologous dendritic cells (DC) vaccines in stimulating an anti-tumor immune response in the young, almost none of these reports consider the effect that aging has on the immune system or test whether DC-vaccination is effective in old hosts. In this study we compared the efficacy of DC-vaccination in young and old mice. Our results showed that DC-vaccination in young animals induced an anti-tumor response resulting in approximately 60% tumor growth inhibition, while minimal protection was observed in old animals. DC vaccination plus rIL-2 further enhanced the anti-tumor response in young animals (approximately 70-75% inhibition), while ineffective in old animals. In contrast, co-administration of anti-OX-40 or anti-4-1BB mAbs vigorously enhanced the anti-tumor immune response in both young (approximately 85-90% inhibition) and old mice (approximately 70-75% inhibition). Our data indicate that although old mice have a decline in immune function, they have the capacity to develop strong anti-tumor responses as long as they are provided with efficient co-stimulation.

  5. Search for the standard model Higgs boson in ZH→ℓ(+)ℓ(-)bb production with the D0 detector in 9.7 fb(-1) of pp collisions at √s = 1.96 TeV.

    PubMed

    Abazov, V M; Abbott, B; Acharya, B S; Adams, M; Adams, T; Alexeev, G D; Alkhazov, G; Alton, A; Alverson, G; Askew, A; Atkins, S; Augsten, K; Avila, C; Badaud, F; Bagby, L; Baldin, B; Bandurin, D V; Banerjee, S; Barberis, E; Baringer, P; Bartlett, J F; Bassler, U; Bazterra, V; Bean, A; Begalli, M; Bellantoni, L; Beri, S B; Bernardi, G; Bernhard, R; Bertram, I; Besançon, M; Beuselinck, R; Bhat, P C; Bhatia, S; Bhatnagar, V; Blazey, G; Blessing, S; Bloom, K; Boehnlein, A; Boline, D; Boos, E E; Borissov, G; Bose, T; Brandt, A; Brandt, O; Brock, R; Bross, A; Brown, D; Brown, J; Bu, X B; Buehler, M; Buescher, V; Bunichev, V; Burdin, S; Buszello, C P; Camacho-Pérez, E; Casey, B C K; Castilla-Valdez, H; Caughron, S; Chakrabarti, S; Chakraborty, D; Chan, K M; Chandra, A; Chapon, E; Chen, G; Chevalier-Théry, S; Cho, D K; Cho, S W; Choi, S; Choudhary, B; Cihangir, S; Claes, D; Clutter, J; Cooke, M; Cooper, W E; Corcoran, M; Couderc, F; Cousinou, M-C; Croc, A; Cutts, D; Das, A; Davies, G; de Jong, S J; De La Cruz-Burelo, E; Déliot, F; Demina, R; Denisov, D; Denisov, S P; Desai, S; Deterre, C; Devaughan, K; Diehl, H T; Diesburg, M; Ding, P F; Dominguez, A; Dubey, A; Dudko, L V; Duggan, D; Duperrin, A; Dutt, S; Dyshkant, A; Eads, M; Edmunds, D; Ellison, J; Elvira, V D; Enari, Y; Evans, H; Evdokimov, A; Evdokimov, V N; Facini, G; Feng, L; Ferbel, T; Fiedler, F; Filthaut, F; Fisher, W; Fisk, H E; Fortner, M; Fox, H; Fuess, S; Garcia-Bellido, A; García-González, J A; García-Guerra, G A; Gavrilov, V; Gay, P; Geng, W; Gerbaudo, D; Gerber, C E; Gershtein, Y; Ginther, G; Golovanov, G; Goussiou, A; Grannis, P D; Greder, S; Greenlee, H; Grenier, G; Gris, Ph; Grivaz, J-F; Grohsjean, A; Grünendahl, S; Grünewald, M W; Guillemin, T; Gutierrez, G; Gutierrez, P; Hagopian, S; Haley, J; Han, L; Harder, K; Harel, A; Hauptman, J M; Hays, J; Head, T; Hebbeker, T; Hedin, D; Hegab, H; Heinson, A P; Heintz, U; Hensel, C; Heredia-De La Cruz, I; Herner, K; Hesketh, G; Hildreth, M D; Hirosky, R; Hoang, T; Hobbs, J D; Hoeneisen, B; Hogan, J; Hohlfeld, M; Howley, I; Hubacek, Z; Hynek, V; Iashvili, I; Ilchenko, Y; Illingworth, R; Ito, A S; Jabeen, S; Jaffré, M; Jayasinghe, A; Jeong, M S; Jesik, R; Jiang, P; Johns, K; Johnson, E; Johnson, M; Jonckheere, A; Jonsson, P; Joshi, J; Jung, A W; Juste, A; Kaadze, K; Kajfasz, E; Karmanov, D; Kasper, P A; Katsanos, I; Kehoe, R; Kermiche, S; Khalatyan, N; Khanov, A; Kharchilava, A; Kharzheev, Y N; Kiselevich, I; Kohli, J M; Kozelov, A V; Kraus, J; Kulikov, S; Kumar, A; Kupco, A; Kurča, T; Kuzmin, V A; Lammers, S; Landsberg, G; Lebrun, P; Lee, H S; Lee, S W; Lee, W M; Lei, X; Lellouch, J; Li, D; Li, H; Li, L; Li, Q Z; Lim, J K; Lincoln, D; Linnemann, J; Lipaev, V V; Lipton, R; Liu, H; Liu, Y; Lobodenko, A; Lokajicek, M; Lopes de Sa, R; Lubatti, H J; Luna-Garcia, R; Lyon, A L; Maciel, A K A; Madar, R; Magaña-Villalba, R; Malik, S; Malyshev, V L; Maravin, Y; Martínez-Ortega, J; McCarthy, R; McGivern, C L; Meijer, M M; Melnitchouk, A; Menezes, D; Mercadante, P G; Merkin, M; Meyer, A; Meyer, J; Miconi, F; Mondal, N K; Mulhearn, M; Nagy, E; Naimuddin, M; Narain, M; Nayyar, R; Neal, H A; Negret, J P; Neustroev, P; Nguyen, H T; Nunnemann, T; Orduna, J; Osman, N; Osta, J; Padilla, M; Pal, A; Parashar, N; Parihar, V; Park, S K; Partridge, R; Parua, N; Patwa, A; Penning, B; Perfilov, M; Peters, Y; Petridis, K; Petrillo, G; Pétroff, P; Pleier, M-A; Podesta-Lerma, P L M; Podstavkov, V M; Popov, A V; Prewitt, M; Price, D; Prokopenko, N; Qian, J; Quadt, A; Quinn, B; Rangel, M S; Ranjan, K; Ratoff, P N; Razumov, I; Renkel, P; Ripp-Baudot, I; Rizatdinova, F; Rominsky, M; Ross, A; Royon, C; Rubinov, P; Ruchti, R; Sajot, G; Salcido, P; Sánchez-Hernández, A; Sanders, M P; Santos, A S; Savage, G; Sawyer, L; Scanlon, T; Schamberger, R D; Scheglov, Y; Schellman, H; Schlobohm, S; Schwanenberger, C; Schwienhorst, R; Sekaric, J; Severini, H; Shabalina, E; Shary, V; Shaw, S; Shchukin, A A; Shivpuri, R K; Simak, V; Skubic, P; Slattery, P; Smirnov, D; Smith, K J; Snow, G R; Snow, J; Snyder, S; Söldner-Rembold, S; Sonnenschein, L; Soustruznik, K; Stark, J; Stoyanova, D A; Strauss, M; Suter, L; Svoisky, P; Takahashi, M; Titov, M; Tokmenin, V V; Tsai, Y-T; Tschann-Grimm, K; Tsybychev, D; Tuchming, B; Tully, C; Uvarov, L; Uvarov, S; Uzunyan, S; Van Kooten, R; van Leeuwen, W M; Varelas, N; Varnes, E W; Vasilyev, I A; Verdier, P; Verkheev, A Y; Vertogradov, L S; Verzocchi, M; Vesterinen, M; Vilanova, D; Vokac, P; Wahl, H D; Wang, M H L S; Warchol, J; Watts, G; Wayne, M; Weichert, J; Welty-Rieger, L; White, A; Wicke, D; Williams, M R J; Wilson, G W; Wobisch, M; Wood, D R; Wyatt, T R; Xie, Y; Yamada, R; Yang, S; Yang, W-C; Yasuda, T; Yatsunenko, Y A; Ye, W; Ye, Z; Yin, H; Yip, K; Youn, S W; Yu, J M; Zennamo, J; Zhao, T; Zhao, T G; Zhou, B; Zhu, J; Zielinski, M; Zieminska, D; Zivkovic, L

    2012-09-21

    We present a search for the standard model (SM) Higgs boson produced in association with a Z boson in 9.7 fb(-1) of pp collisions collected with the D0 detector at the Fermilab Tevatron Collider at √s = 1.96 TeV. Selected events contain one reconstructed Z→e(+)e(-) or Z→μ(+)μ(-) candidate and at least two jets, including at least one jet identified as likely to contain a b quark. To validate the search procedure, we also measure the cross section for ZZ production in the same final state. It is found to be consistent with its SM prediction. We set upper limits on the ZH production cross section times branching ratio for H→bb at the 95% C.L. for Higgs boson masses 90 ≤ M(H) ≤ 150 GeV. The observed (expected) limit for M(H) = 125 GeV is 7.1 (5.1) times the SM cross section.

  6. Third-generation CD28/4-1BB chimeric antigen receptor T cells for chemotherapy relapsed or refractory acute lymphoblastic leukaemia: a non-randomised, open-label phase I trial protocol

    PubMed Central

    Tang, Xiao-Yi; Sun, Yao; Zhang, Ang; Hu, Guo-Liang; Cao, Wei; Wang, Dan-Hong; Zhang, Bin; Chen, Hu

    2016-01-01

    Introduction There is no curative treatment available for patients with chemotherapy relapsed or refractory CD19+ B cells-derived acute lymphoblastic leukaemia (r/r B-ALL). Although CD19-targeting second-generation (2nd-G) chimeric antigen receptor (CAR)-modified T cells carrying CD28 or 4-1BB domains have demonstrated potency in patients with advanced B-ALL, these 2 signalling domains endow CAR-T cells with different and complementary functional properties. Preclinical results have shown that third-generation (3rd-G) CAR-T cells combining 4-1BB and CD28 signalling domains have superior activation and proliferation capacity compared with 2nd-G CAR-T cells carrying CD28 domain. The aim of the current study is therefore to investigate the safety and efficacy of 3rd-G CAR-T cells in adults with r/r B-ALL. Methods and analysis This study is a phase I clinical trial for patients with r/r B-ALL to test the safety and preliminary efficacy of 3rd-G CAR-T cells. Before receiving lymphodepleting conditioning regimen, the peripheral blood mononuclear cells from eligible patients will be leukapheresed, and the T cells will be purified, activated, transduced and expanded ex vivo. On day 6 in the protocol, a single dose of 1 million CAR-T cells per kg will be administrated intravenously. The phenotypes of infused CAR-T cells, copy number of CAR transgene and plasma cytokines will be assayed for 2 years after CAR-T infusion using flow cytometry, real-time quantitative PCR and cytometric bead array, respectively. Moreover, several predictive plasma cytokines including interferon-γ, interleukin (IL)-6, IL-8, Soluble Interleukin (sIL)-2R-α, solubleglycoprotein (sgp)130, sIL-6R, Monocyte chemoattractant protein (MCP1), Macrophage inflammatory protein (MIP1)-α, MIP1-β and Granulocyte-macrophage colony-stimulating factor (GM-CSF), which are highly associated with severe cytokine release syndrome (CRS), will be used to forecast CRS to allow doing earlier intervention, and CRS will

  7. Umbilical cord blood regulatory T-cell expansion and functional effects of tumor necrosis factor receptor family members OX40 and 4-1BB expressed on artificial antigen-presenting cells

    PubMed Central

    Harker-Murray, Paul; Porter, Stephen B.; Merkel, Sarah C.; Londer, Aryel; Taylor, Dawn K.; Bina, Megan; Panoskaltsis-Mortari, Angela; Rubinstein, Pablo; Van Rooijen, Nico; Golovina, Tatiana N.; Suhoski, Megan M.; Miller, Jeffrey S.; Wagner, John E.; June, Carl H.; Riley, James L.

    2008-01-01

    Previously, we showed that human umbilical cord blood (UCB) regulatory T cells (Tregs) could be expanded approximately 100-fold using anti-CD3/28 monoclonal antibody (mAb)–coated beads to provide T-cell receptor and costimulatory signals. Because Treg numbers from a single UCB unit are limited, we explored the use of cell-based artificial antigen-presenting cells (aAPCs) preloaded with anti-CD3/28 mAbs to achieve higher levels of Treg expansion. Compared with beads, aAPCs had similar expansion properties while significantly increasing transforming growth factor β (TGF-β) secretion and the potency of Treg suppressor function. aAPCs modified to coexpress OX40L or 4-1BBL expanded UCB Tregs to a significantly greater extent than bead- or nonmodified aAPC cultures, reaching mean expansion levels exceeding 1250-fold. Despite the high expansion and in contrast to studies using other Treg sources, neither OX40 nor 4-1BB signaling of UCB Tregs reduced in vitro suppression. UCB Tregs expanded with 4-1BBL expressing aAPCs had decreased levels of proapoptotic bim. UCB Tregs expanded with nonmodified or modified aAPCs versus beads resulted in higher survival associated with increased Treg persistence in a xeno-geneic graft-versus-host disease lethality model. These data offer a novel approach for UCB Treg expansion using aAPCs, including those coexpressing OX40L or 4-1BBL. PMID:18645038

  8. Diagnostic accuracy of heart fatty acid binding protein (H-FABP) and glycogen phosphorylase isoenzyme BB (GPBB) in diagnosis of acute myocardial infarction in patients with acute coronary syndrome

    PubMed Central

    Cubranic, Zlatko; Madzar, Zeljko; Matijevic, Sanja; Dvornik, Stefica; Fisic, Elizabeta; Tomulic, Vjekoslav; Kunisek, Juraj; Laskarin, Gordana; Kardum, Igor; Zaputovic, Luka

    2012-01-01

    Introduction: This study aimed to assess whether heart fatty acid-binding protein (H-FABP) and glycogen phosphorylase isoenzyme BB (GPBB) could be used for the accurate diagnosis of acute myocardial infarction (AMI) in acute coronary syndrome (ACS) patients. Materials and methods: The study included 108 ACS patients admitted to a coronary unit within 3 h after chest pain onset. AMI was distinguished from unstable angina (UA) using a classical cardiac troponin I (cTnI) assay. H-FABP and GPBB were measured by ELISA on admission (0 h) and at 3, 6, 12, and 24 h after admission; their accuracy to diagnose AMI was assessed using statistical methods. Results: From 92 patients with ACS; 71 had AMI. H-FABP and GPBB had higher peak value after 3 h from admission than cTnI (P = 0.001). Both markers normalized at 24 h. The area under the receiver operating characteristic curves was significantly greater for both markers in AMI patients than in UA patients at all time points tested, including admission (P < 0.001). At admission, the H-FABP (37%) and GPBB (40%) sensitivities were relatively low. They increased at 3 and 6 h after admission for both markers and decreased again after 24 h. It was 40% for H-FABP and approximately 2-times lower for GPBB (P < 0.01). In AMI patients, both biomarkers had similar specificities, positive- and negative-predictive values, positive and negative likelihood ratios, and risk ratios for AIM. Conclusion: H-FABP and GPBB can contribute to early AMI diagnosis and can distinguish AMI from UA. PMID:22838188

  9. Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 modulate gene expression of subset specific transcription factors and cytokines in peripheral blood mononuclear cells of obese and overweight people.

    PubMed

    Zarrati, Mitra; Shidfar, Farzad; Nourijelyani, Keramat; Mofid, Vahid; Hossein zadeh-Attar, Mohammad Javad; Bidad, Katayoon; Najafi, Forouzan; Gheflati, Zahra; Chamari, Maryam; Salehi, Eisa

    2013-01-01

    Probiotics are believed to have interaction with immune cells through sustained effects on gene expression of different cytokines and transcription factors. The present randomized doubled-blind controlled clinical trial was performed recruiting 75 individuals with BMI 25-35, who were randomly assigned to the following three groups: Group 1 (n = 25) who consumed regular yogurt as part of a low calorie diet [RLCD], group 2 (n = 25) who received probiotic yogurt with a LCD [PLCD] and group 3 (n = 25) who consumed probiotic yogurt without LCD [PWLCD] for 8 week. Participants in PLCD and PWLCD groups received 200 g/day yogurt containing Lactobacillus acidophilus La5, Bifidobacterium Bb12, and lactobacillus casei DN001 10(8) cfu/gr. The expression of the FOXP3, T-bet, GATA3, TNF-α, IFN-γ, TGF-β, and ROR-γt in PBMCs genes were assessed, before and after intervention. In three groups, ROR-γt expression was reduced (P = 0.007) and FOXP3 was increased (P < 0.001). The expression of TNFα, TGFβ, and GATA3 genes did not change among all groups after intervention. Interestingly, the expression of T-bet gene, which was significantly decreased in PLCD and PWLCD groups (P < 0.001), whereas gene expression of IFN-γ decreased in all three groups. Our results suggest that weight loss diet and probiotic yogurt had synergistic effects on T-cell subset specific gene expression in peripheral blood mononuclear cells among overweight and obese individuals.

  10. BB Potentials in Quenched Lattice QCD

    SciTech Connect

    William Detmold; Kostas Orginos; Martin J. Savage

    2007-12-01

    The potentials between two B-mesons are computed in the heavy-quark limit using quenched lattice QCD at $m_\\pi\\sim 400~{\\rm MeV}$. Non-zero central potentials are clearly evident in all four spin-isospin channels, (I,s_l) = (0,0) , (0,1) , (1,0) , (1,1), where s_l is the total spin of the light degrees of freedom. At short distance, we find repulsion in the $I\

  11. Chorioretinitis sclopetaria from BB ex memoria.

    PubMed

    Otto, C S; Nixon, K L; Mazzoli, R A; Raymond, W R; Ainbinder, D J; Hansen, E A; Krolicki, T J

    2001-01-01

    Chorioretinitis sclopetaria presents a characteristic pattern of choroidal and retinal changes caused by a high velocity projectile passing into the orbit, in close proximity to the globe. While it is unlikely that a patient should completely forget the trauma causing such damage, preserved or compensated visual function may blur the patient's memory of these events over time. Characteristic physical findings help to clarify the antecedent history. Despite the lack of an acknowledged history of ocular trauma or surgery, in our case, the characteristic ocular findings discovered at presentation allowed for recognition of the underlying etiology. Because of good visual function, the patient had completely forgotten about the trauma that occurred 12 years earlier. Strabismus surgery was performed for treatment of the presenting symptomatic diplopia. The pathognomonic findings in chorioretinitis sclopetaria are invaluable in correctly diagnosing this condition, especially when a history of ocular trauma is unavailable.

  12. Biochemical and kinetic characterisation of a novel xylooligosaccharide-upregulated GH43 β-d-xylosidase/α-l-arabinofuranosidase (BXA43) from the probiotic Bifidobacterium animalis subsp. lactis BB-12

    PubMed Central

    2013-01-01

    The Bifidobacterium animalis subsp. lactis BB-12 gene BIF_00092, assigned to encode a β-d-xylosidase (BXA43) of glycoside hydrolase family 43 (GH43), was cloned with a C-terminal His-tag and expressed in Escherichia coli. BXA43 was purified to homogeneity from the cell lysate and found to be a dual-specificity exo-hydrolase active on para-nitrophenyl-β-d-xylopyranoside (pNPX), para-nitrophenyl-α-L-arabinofuranoside (pNPA), β-(1 → 4)-xylopyranosyl oligomers (XOS) of degree of polymerisation (DP) 2–4, and birchwood xylan. A phylogenetic tree of the 92 characterised GH43 enzymes displayed five distinct groups (I − V) showing specificity differences. BXA43 belonged to group IV and had an activity ratio for pNPA:pNPX of 1:25. BXA43 was stable below 40°C and at pH 4.0–8.0 and showed maximum activity at pH 5.5 and 50°C. Km and kcat for pNPX were 15.6 ± 4.2 mM and 60.6 ± 10.8 s-1, respectively, and substrate inhibition became apparent above 18 mM pNPX. Similar kinetic parameters and catalytic efficiency values were reported for β-d-xylosidase (XynB3) from Geobacillus stearothermophilus T‒6 also belonging to group IV. The activity of BXA43 for xylooligosaccharides increased with the size and was 2.3 and 5.6 fold higher, respectively for xylobiose and xylotetraose compared to pNPX. BXA43 showed clearly metal inhibition for Zn2+ and Ag+, which is different to its close homologues. Multiple sequence alignment and homology modelling indicated that Arg505Tyr506 present in BXA43 are probably important for binding to xylotetraose at subsite +3 and occur only in GH43 from the Bifidobacterium genus. PMID:24025736

  13. A Generally Applicable Computer Algorithm Based on the Group Additivity Method for the Calculation of Seven Molecular Descriptors: Heat of Combustion, LogPO/W, LogS, Refractivity, Polarizability, Toxicity and LogBB of Organic Compounds; Scope and Limits of Applicability.

    PubMed

    Naef, Rudolf

    2015-10-07

    A generally applicable computer algorithm for the calculation of the seven molecular descriptors heat of combustion, logPoctanol/water, logS (water solubility), molar refractivity, molecular polarizability, aqueous toxicity (protozoan growth inhibition) and logBB (log (cblood/cbrain)) is presented. The method, an extendable form of the group-additivity method, is based on the complete break-down of the molecules into their constituting atoms and their immediate neighbourhood. The contribution of the resulting atom groups to the descriptor values is calculated using the Gauss-Seidel fitting method, based on experimental data gathered from literature. The plausibility of the method was tested for each descriptor by means of a k-fold cross-validation procedure demonstrating good to excellent predictive power for the former six descriptors and low reliability of logBB predictions. The goodness of fit (Q²) and the standard deviation of the 10-fold cross-validation calculation was >0.9999 and 25.2 kJ/mol, respectively, (based on N = 1965 test compounds) for the heat of combustion, 0.9451 and 0.51 (N = 2640) for logP, 0.8838 and 0.74 (N = 1419) for logS, 0.9987 and 0.74 (N = 4045) for the molar refractivity, 0.9897 and 0.77 (N = 308) for the molecular polarizability, 0.8404 and 0.42 (N = 810) for the toxicity and 0.4709 and 0.53 (N = 383) for logBB. The latter descriptor revealing a very low Q² for the test molecules (R² was 0.7068 and standard deviation 0.38 for N = 413 training molecules) is included as an example to show the limits of the group-additivity method. An eighth molecular descriptor, the heat of formation, was indirectly calculated from the heat of combustion data and correlated with published experimental heat of formation data with a correlation coefficient R² of 0.9974 (N = 2031).

  14. Search for the Standard Model Higgs Boson in ZH→l⁺l⁻bb̄ Production with the D0 Detector in 9.7 fb⁻¹ of pp̄ Collisions at √s=1.96 TeV

    DOE PAGES

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; ...

    2012-09-20

    We present a search for the standard model (SM) Higgs boson produced in association with a Z boson in 9.7 fb⁻¹ of pp̄ collisions collected with the D0 detector at the Fermilab Tevatron Collider at √s=1.96 TeV. Selected events contain one reconstructed Z→e⁺e⁻ or Z→μ⁺μ⁻ candidate and at least two jets, including at least one jet identified as likely to contain a b quark. To validate the search procedure, we also measure the cross section for ZZ production in the same final state. It is found to be consistent with its SM prediction. We set upper limits on the ZHmore » production cross section times branching ratio for H→bb̄ at the 95% C.L. for Higgs boson masses 90≤MH≤150 GeV. The observed (expected) limit for MH=125 GeV is 7.1 (5.1) times the SM cross section.« less

  15. Search for the Standard Model Higgs Boson in ZH→l⁺l⁻bb̄ Production with the D0 Detector in 9.7 fb⁻¹ of pp̄ Collisions at √s=1.96 TeV

    SciTech Connect

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Alverson, G.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Bose, T.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Brown, J.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chakraborty, D.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Chevalier-Théry, S.; Cho, D. K.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M.-C.; Croc, A.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duggan, D.; Duperrin, A.; Dutt, S.; Dyshkant, A.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, A.; Evdokimov, V. N.; Facini, G.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garcia-Bellido, A.; García-González, J. A.; García-Guerra, G. A.; Gavrilov, V.; Gay, P.; Geng, W.; Gerbaudo, D.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Golovanov, G.; Goussiou, A.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J.-F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Hagopian, S.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kaadze, K.; Kajfasz, E.; Karmanov, D.; Kasper, P. A.; Katsanos, I.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kulikov, S.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Landsberg, G.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Lubatti, H. J.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Maravin, Y.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Naimuddin, M.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Padilla, M.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M.-A.; Podesta-Lerma, P. L. M.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Rangel, M. S.; Ranjan, K.; Ratoff, P. N.; Razumov, I.; Renkel, P.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Salcido, P.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schlobohm, S.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Shivpuri, R. K.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Smith, K. J.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Takahashi, M.; Titov, M.; Tokmenin, V. V.; Tsai, Y.-T.; Tschann-Grimm, K.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verdier, P.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; White, A.; Wicke, D.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yang, W.-C.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2012-09-20

    We present a search for the standard model (SM) Higgs boson produced in association with a Z boson in 9.7 fb⁻¹ of pp̄ collisions collected with the D0 detector at the Fermilab Tevatron Collider at √s=1.96 TeV. Selected events contain one reconstructed Z→e⁺e⁻ or Z→μ⁺μ⁻ candidate and at least two jets, including at least one jet identified as likely to contain a b quark. To validate the search procedure, we also measure the cross section for ZZ production in the same final state. It is found to be consistent with its SM prediction. We set upper limits on the ZH production cross section times branching ratio for H→bb̄ at the 95% C.L. for Higgs boson masses 90≤MH≤150 GeV. The observed (expected) limit for MH=125 GeV is 7.1 (5.1) times the SM cross section.

  16. On the source of the flaring activity in AB Doradus: the UV spectral signatures

    NASA Astrophysics Data System (ADS)

    Gómez de Castro, Ana I.

    2002-05-01

    AB Dor is a young, 30-Myr-old, low-mass star with a short rotation period and strong flaring activity. In this work, a detailed analysis of the flaring activity is carried out based on ultraviolet spectral tracers of hot (T ~105 ) plasma, namely HST /GHRS profiles of the Civ[uv1] and Siiv[uv1] lines. The quiescent component has been subtracted out and the spectral signature of the flares is analysed in detail. A total of nine events are detected in 10.63h. The e-folding time for flares has been derived when possible, yielding values between 300 and 1200s. It is shown that the Civ[uv1] and Siiv[uv1] profiles associated with the flaring activity can be classified into three main groups: narrow redshifted profiles, `broad' profiles and double-peaked profiles. Therefore, the spectroscopic signature of flares in hot (T ~105 K) plasma is not always the same. As a consequence, it is highly likely that different mechanisms are involved in the flaring activity of AB Dor. The most frequently observed profiles are the narrow redshifted profiles; six out of the nine events display this type of profile. An analysis of the velocity field at the stellar surface shows up that they are most likely associated with matter infall. Henceforth, it is feasible that this type of flares are associated with downward flowing material in post-flare magnetic loops , as frequently observed in the Sun. The broad profiles have a FWHM~=300kms-1 and are very asymmetric with an extended blue wing reaching 400kms-1 . These profiles have been observed during the strongest flare in the monitoring when the line flux rose up to three times the quiescent value. The large width and asymmetry of the profiles are best explained if this type of flares are associated with gas flows in curved magnetic structures. Three possible mechanisms are analysed: (1) the development of thermal instabilities in large magnetic loops, (2) gas infall and (3) the interaction between the fast and slow components of the stellar wind. The last mechanism seems to be the most feasible given the short duration of the flares. Finally, a possible absorption caused by the hot component of `sling-shot' prominences has been searched for in the Civ[uv1] and Siiv[uv1] profiles. Two of the three strongest Hα prominences (A and H) seem to have been detected. The most compelling evidence corresponds to A, when an absorption component is observed moving from blue to redshifted velocities from φ =10440.978 to φ =10441.010. The strength of the absorption at φ =10441.010 is 0.2×10-12 ergs-1 cm-2 and has a FWHM=86kms-1 .

  17. Accurate radio and optical positions for the radio star HD 36705 (AB Doradus)

    NASA Technical Reports Server (NTRS)

    White, Graeme L.; Jauncey, David L.; Batty, Michael J.; Peters, W. L.; Gulkis, S.

    1988-01-01

    Arc-second position measurements of the active star HD 36705 (AB Dor) and of the variable radio source found nearby are presented. These measurements show that the radio source is clearly identified with HD 36705 and not with the nearby red-dwarf star Rst 137B.

  18. Studies at high frequencies of the 30 Doradus and RCW 57 regions.

    NASA Astrophysics Data System (ADS)

    Sabalisck, N. S. P.; Abraham, Z.

    1990-11-01

    The authors present maps of the 30 Dor region at 22 GHz and of the RCW 57 region at 43 GHz. The data are compared with maps at lower frequencies and similar resolutions. In the 30 Dor region 3 supernova remnants of plerionic type were detected: N 1578, MC 78 and MC 89.

  19. R136 - the Core of the Ionizing Cluster of 30-DORADUS

    NASA Astrophysics Data System (ADS)

    Melnick, J.

    1983-06-01

    I first read that R136 contained a supermassive object some time ago in the Sunday edition of the Chilean daily newspaper EI Mercurio, where it was announced that ... "European astronomers discover the most massive star in the Universe!". Since EI Mercurio is almost always wrang I did not take the announcement very seriously until the paper by Feitzinger and Co-workers (henceforth the Bochum graup) appeared in Astronomy and Astrophysics in 1980. More or less simultaneously with the publication of these optical observations, a graup of observers from the University of Wisconsin, headed by J. Cassinelli (henceforth the Wisconsin group) reached the same Conclusion on the basis of ultraviolet observations obtained with the International Ultraviolet Explorer (IUE).

  20. The nature of R136a, the superluminous central object of the 30 Doradus nebula

    NASA Technical Reports Server (NTRS)

    Cassinelli, J. P.; Savage, B. D.; Fitzpatrick, E. L.; Ebbets, D. C.

    1983-01-01

    UV data show R136a to be an extremely luminous object having spectral characteristics similar to that of O3 stars. It is suggested that the bright component of R136a is either a single, supermassive hydrogen-burning star, or a compact multiple system of supermassive stars. In the former case, the observational data imply a mass exceeding that of the most massive stars known by a factor of 20 and raise important theoretical problems concerning stellar formation and stability. Formation may occur through ordinary collapse in a region with peculiar dust properties, or through coalescence in the core of a compact cluster.

  1. Hubble Tarantula Treasury Project V. The Star Cluster Hodge 301: The Old Face of 30 Doradus

    NASA Astrophysics Data System (ADS)

    Cignoni, M.; Sabbi, E.; van der Marel, R. P.; Lennon, D. J.; Tosi, M.; Grebel, E. K.; Gallagher, J. S., III; Aloisi, A.; de Marchi, G.; Gouliermis, D. A.; Larsen, S.; Panagia, N.; Smith, L. J.

    2016-12-01

    Based on color-magnitude diagrams (CMDs) from the Hubble Space Telescope Hubble Tarantula Treasury Project (HTTP) survey, we present the star formation history of Hodge 301, the oldest star cluster in the Tarantula Nebula. The HTTP photometry extends faint enough to reach, for the first time, the cluster pre-main sequence (PMS) turn-on, where the PMS joins the main sequence. Using the location of this feature, along with synthetic CMDs generated with the latest PARSEC models, we find that Hodge 301 is older than previously thought, with an age between 26.5 and 31.5 Myr. From this age, we also estimate that between 38 and 61 Type II supernovae exploded in the region. The same age is derived from the main sequence turn-off, whereas the age derived from the post-main sequence stars is younger and between 20 and 25 Myr. Other relevant parameters are a total stellar mass of ≈8800 ± 800 M ⊙ and average reddening E(B - V) ≈ 0.22-0.24 mag, with a differential reddening δE(B - V) ≈ 0.04 mag. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA Inc., under NASA contract NAS 5-26555.

  2. Near-uniform internal rotation of the main-sequence γ Doradus pulsator KIC 7661054

    NASA Astrophysics Data System (ADS)

    Murphy, Simon J.; Fossati, Luca; Bedding, Timothy R.; Saio, Hideyuki; Kurtz, Donald W.; Grassitelli, Luca; Wang, Edric S.

    2016-06-01

    We used Kepler photometry to determine the internal rotation rate of KIC 7661054, a chemically normal γ Dor star on the main sequence at spectral type F2.5 V. The core rotation period of 27.25 ± 0.06 d is obtained from the rotational splittings of a series of dipole g modes. The surface rotation period is calculated from a spectroscopic projected rotation velocity and a stellar radius computed from models. Literature data, obtained without inclusion of macroturbulence as a line-broadening mechanism, imply that the surface rotates much more quickly than the core, while our detailed analysis suggests that the surface may rotate slightly more quickly than the core and that the rotation profile is uniform within the 1σ uncertainties. We discuss the pitfalls associated with the determination of surface rotation rates of slow rotators from spectroscopy in the absence of asteroseismic constraints. A broad signal is observed at low frequency, which we show cannot be attributed to rotation, contrary to previous suggestions concerning the origin of such signals.

  3. Kepler Eclipsing Binaries with Delta Scuti/Gamma Doradus Pulsating Components I: KIC 9851944

    NASA Astrophysics Data System (ADS)

    Guo, Zhao; Gies, Douglas R.; Matson, Rachel A.; García Hernández, Antonio

    2016-07-01

    KIC 9851944 is a short-period (P = 2.16 days) eclipsing binary in the Kepler field of view. By combining the analysis of Kepler photometry and phase-resolved spectra from Kitt Peak National Observatory and Lowell Observatory, we determine the atmospheric and physical parameters of both stars. The two components have very different radii (2.27 R ⊙, 3.19 R ⊙) but close masses (1.76 M ⊙, 1.79 M ⊙) and effective temperatures (7026, 6902 K), indicating different evolutionary stages. The hotter primary is still on the main sequence (MS), while the cooler and larger secondary star has evolved to the post-MS, burning hydrogen in a shell. A comparison with coeval evolutionary models shows that it requires solar metallicity and a higher mass ratio to fit the radii and temperatures of both stars simultaneously. Both components show δ Scuti-type pulsations, which we interpret as p-modes and p and g mixed modes. After a close examination of the evolution of δ Scuti pulsational frequencies, we make a comparison of the observed frequencies with those calculated from MESA/GYRE.

  4. Brown Dwarfs in Young Moving Groups from Pan-STARRS1. I. AB Doradus

    NASA Astrophysics Data System (ADS)

    Aller, Kimberly M.; Liu, Michael C.; Magnier, Eugene A.; Best, William M. J.; Kotson, Michael C.; Burgett, William S.; Chambers, Kenneth C.; Hodapp, Klaus W.; Flewelling, Heather; Kaiser, Nick; Metcalf, Nigel; Tonry, John L.; Wainscoat, Richard J.; Waters, Christopher

    2016-04-01

    Substellar members of young (≲150 Myr) moving groups are valuable benchmarks to empirically define brown dwarf evolution with age and to study the low-mass end of the initial mass function. We have combined Pan-STARRS1 (PS1) proper motions with optical-IR photometry from PS1, Two Micron All Sky Survey (2MASS), and WISE to search for substellar members of the AB Dor Moving Group within ≈50 pc and with spectral types of late M to early L, corresponding to masses down to ≈30 MJup at the age of the group (≈125 Myr). Including both photometry and proper motions allows us to better select candidates by excluding field dwarfs whose colors are similar to young AB Dor Moving Group members. Our near-IR spectroscopy has identified six ultracool dwarfs (M6-L4 ≈30-100 MJup) with intermediate surface gravities (int-g) as candidate members of the AB Dor Moving Group. We find another two candidate members with spectra showing hints of youth but consistent with field gravities. We also find four field brown dwarfs unassociated with the AB Dor Moving Group, three of which have int-g gravity classification. While signatures of youth are present in the spectra of our ≈125 Myr objects, neither their J - K nor W1 - W2 colors are significantly redder than field dwarfs with the same spectral types, unlike younger ultracool dwarfs. We also determined PS1 parallaxes for eight of our candidates and one previously identified AB Dor Moving Group candidate. Although radial velocities (and parallaxes, for some) are still needed to fully assess membership, these new objects provide valuable insight into the spectral characteristics and evolution of young brown dwarfs.

  5. Looking on the bright side: The story of AA Doradus as revealed by its cool companion

    NASA Astrophysics Data System (ADS)

    Vučković, M.; Østensen, R. H.; Németh, P.; Bloemen, S.; Pápics, P. I.

    2016-02-01

    The effects of irradiation on the secondary stars of close binary systems are crucial for reliably determining the system parameters and for understanding the close binary evolution. They affect the stellar structure of the irradiated star and are reflected in the appearance of characteristic features in the spectroscopic and photometric data of these systems. We aim to study the light that originates from the irradiated side of the low-mass component of a close binary eclipsing system, which comprises a hot subdwarf primary and a low mass companion, to precisely interpret their high precision photometric and spectroscopic data, and accurately determine their system and surface parameters. We reanalyse the archival high-resolution time-resolved VLT/UVES spectra of AA Dor system, where irradiation features have already been detected. After removing the predominant contribution of the hot subdwarf primary, the residual spectra reveal more than 100 emission lines from the heated side of the secondary, which show maximum intensity close to the phases around the secondary eclipse. We analyse the residual spectrum to model the irradiation of the low-mass secondary. We perform a detailed analysis of 22 narrow emission lines of the irradiated secondary, mainly of O ii, with a few significant C ii lines. Their phase profiles constrain the emission region of the heated side to a radius ≥95% of the radius of the secondary, while the shape of their velocity profiles reveals two distinct asymmetry features, one at the quadrature and the other at the secondary eclipse. In addition, we identify weaker emission signatures originating from more than 70 lines, including lines from He i, N ii, Si iii, Ca ii, and Mg ii. From the emission lines of the heated side of the secondary star, we determine the radial velocity semi-amplitude of the centre-of-light and correct it to the centre-of-mass of the secondary which, in turn, gives accurate masses of both components of the AA Dor system. The resulting masses M1 = 0.46 ± 0.01 M⊙ and M2 = 0.079 ± 0.002 M⊙ are in perfect accordance with those of a canonical hot subdwarf primary and a low mass that is just at the substellar limit for the companion. We also compute a first generation atmosphere model of the low mass secondary, which includes irradiation effects and matches the observed spectrum well. We find an indication of an extended atmosphere of the irradiated secondary star. Based on observations collected at the European Southern Observatory, Chile. Program ID: 066.D-1800.

  6. The eclipsing system V404 Lyr: Light-travel times and γ Doradus pulsations

    SciTech Connect

    Lee, Jae Woo; Kim, Seung-Lee; Hong, Kyeongsoo; Lee, Chung-Uk; Koo, Jae-Rim E-mail: slkim@kasi.re.kr E-mail: leecu@kasi.re.kr

    2014-08-01

    We present the physical properties of V404 Lyr exhibiting eclipse timing variations and multiperiodic pulsations from all historical data including the Kepler and SuperWASP observations. Detailed analyses of 2922 minimum epochs showed that the orbital period has varied through a combination of an upward-opening parabola and two sinusoidal variations, with periods of P {sub 3} = 649 days and P {sub 4} = 2154 days and semi-amplitudes of K {sub 3} = 193 s and K {sub 4} = 49 s, respectively. The secular period increase at a rate of +1.41 × 10{sup –7} days yr{sup –1} could be interpreted as a combination of the secondary to primary mass transfer and angular momentum loss. The most reasonable explanation for both sinusoids is a pair of light-travel-time effects due to two circumbinary objects with projected masses of M {sub 3} = 0.47 M {sub ☉} and M {sub 4} = 0.047 M {sub ☉}. The third-body parameters are consistent with those calculated using the Wilson-Devinney binary code. For the orbital inclinations i {sub 4} ≳ 43°, the fourth component has a mass within the hydrogen-burning limit of ∼0.07 M {sub ☉}, which implies that it is a brown dwarf. A satisfactory model for the Kepler light curves was obtained by applying a cool spot to the secondary component. The results demonstrate that the close eclipsing pair is in a semi-detached, but near-contact, configuration; the primary fills approximately 93% of its limiting lobe and is larger than the lobe-filling secondary. Multiple frequency analyses were applied to the light residuals after subtracting the synthetic eclipsing curve from the Kepler data. This revealed that the primary component of V404 Lyr is a γ Dor type pulsating star, exhibiting seven pulsation frequencies in the range of 1.85-2.11 day{sup –1} with amplitudes of 1.38-5.72 mmag and pulsation constants of 0.24-0.27 days. The seven frequencies were clearly identified as high-order low-degree gravity-mode oscillations which might be excited through tidal interaction. Only eight eclipsing binaries have been known to contain γ Dor pulsating components and, therefore, V404 Lyr will be an important test bed for investigating these rare and interesting objects.

  7. Testing Theoretical Evolutionary Models with AB Doradus C and the Initial Mass Function

    NASA Astrophysics Data System (ADS)

    Luhman, K. L.; Potter, D.

    2006-02-01

    We assess the constraints on the evolutionary models of young low-mass objects that are provided by the measurements of the companion AB Dor C by Close and coworkers and by a new comparison of model-derived IMFs of star-forming regions to the well-calibrated IMF of the solar neighborhood. After performing an independent analysis of all of the imaging and spectroscopic data for AB Dor C that were obtained by Close, we find that AB Dor C (which has no methane) is not detected at a significant level (S/N~1.2) in the SDI data when one narrowband image is subtracted from another but that it does appear in the individual SDI frames, as well as the images at J, H, and Ks. Although our broadband photometry for AB Dor C is consistent with that of Close, the uncertainties that we measure are larger. Using the age of τ=75-150 Myr recently estimated for AB Dor by Luhman and coworkers, the luminosity predicted by the models of Chabrier and Baraffe is consistent with the value that we estimate from the photometry for AB Dor C. We measure a spectral type of M6+/-1 from the K-band spectrum of AB Dor C, which is earlier than the value of M8+/-1 reported by Close and is consistent with the model predictions when a dwarf temperature scale is adopted. In a test of these evolutionary models at much younger ages, we show that the low-mass IMFs that they produce for star-forming regions are similar to the IMF of the solar neighborhood. If the masses of the low-mass stars and brown dwarfs in these IMFs of star-forming regions were underestimated by a factor of 2 as suggested by Close, then the IMF characterizing the current generation of Galactic star formation would have to be radically different from the IMF of the solar neighborhood. Based on observations made with ESO telescopes at the Paranal Observatories under program ID 60.A-9026. This publication makes use of data products from the Two Micron All Sky Survey, which is a joint project of the University of Massachusetts and the Infrared Processing and Analysis Center/California Institute of Technology, funded by the National Aeronautics and Space Administration and the National Science Foundation.

  8. Extracting bb Higgs Decay Signals using Multivariate Techniques

    SciTech Connect

    Smith, W Clarke; /George Washington U. /SLAC

    2012-08-28

    For low-mass Higgs boson production at ATLAS at {radical}s = 7 TeV, the hard subprocess gg {yields} h{sup 0} {yields} b{bar b} dominates but is in turn drowned out by background. We seek to exploit the intrinsic few-MeV mass width of the Higgs boson to observe it above the background in b{bar b}-dijet mass plots. The mass resolution of existing mass-reconstruction algorithms is insufficient for this purpose due to jet combinatorics, that is, the algorithms cannot identify every jet that results from b{bar b} Higgs decay. We combine these algorithms using the neural net (NN) and boosted regression tree (BDT) multivariate methods in attempt to improve the mass resolution. Events involving gg {yields} h{sup 0} {yields} b{bar b} are generated using Monte Carlo methods with Pythia and then the Toolkit for Multivariate Analysis (TMVA) is used to train and test NNs and BDTs. For a 120 GeV Standard Model Higgs boson, the m{sub h{sup 0}}-reconstruction width is reduced from 8.6 to 6.5 GeV. Most importantly, however, the methods used here allow for more advanced m{sub h{sup 0}}-reconstructions to be created in the future using multivariate methods.

  9. ASASSN-17bb: Discovery of a Probable Supernova

    NASA Astrophysics Data System (ADS)

    Brimacombe, J.; Brown, J. S.; Stanek, K. Z.; Holoien, T. W.-S.; Kochanek, C. S.; Shields, J.; Thompson, T. A.; Shappee, B. J.; Prieto, J. L.; Bersier, D.; Dong, Subo; Bose, S.; Chen, Ping; Koff, R. A.; Masi, G.; Post, R. S.; Stone, G.

    2017-01-01

    During the ongoing All Sky Automated Survey for SuperNovae (ASAS-SN or "Assassin"), using data from the quadruple 14-cm "Brutus" telescope in Haleakala, Hawaii, we discovered a new transient sources, most likely a supernova, in the galaxy 2MASX J15204087+043933.

  10. bb¯ spectroscopy from the Υ(3S) state

    NASA Astrophysics Data System (ADS)

    Heintz, U.; Lee-Franzini, J.; Lovelock, D. M. J.; Narain, M.; Schamberger, R. D.; Willins, J.; Yanagisawa, C.; Franzini, P.; Kanekal, S.; Tuts, P. M.; Wu, Q. W.

    1992-09-01

    Using the CUSB-II detector at CESR we have observed electric dipole transitions between the Υ and χb states, both in exclusive and inclusive channels. We have measured their branching ratios and find B(Υ(3S)-->χb(2P2,1,0)γ)=(11.1+/-0.5+/-0.4)%, (11.5+/-0.5+/-0.5)%, (6.0+/-0.4+/-0.6)%. We have measured the center of gravity of the χb(2P) states to be (10259.5+/-0.4+/-1.0) MeV and their fine-structure mass splittings to be (13.5+/-0.4+/-0.5) MeV between the J=2 and J=1 states and (23.5+/-0.7+/-0.7) MeV between J=1 and J=0, leading to a fine-structure ratio of 0.574+/-0.024+/-0.02. The measured fine-structure splitting implies that the spin-orbit interaction dominates over the tensor interaction and that the long-range confining potential is due to an effective scalar exchange. From the measured branching ratios we infer the hadronic widths of the χb(2P) states and find them to be consistent with QCD predictions. We use them to derive values of αs. We have observed the suppressed transition Υ(3S)-->χb(1P)γ and the π0π0 transitions from the Υ(3S). We find B(Υ(3S)-->Υ(1S)π0π0)=(2.2+/-0.4+/-0.3)% and B(Υ(3S)-->Υ(2S)π0π0)=(1.7+/-0.5+/-0.2)%. We also present a measurement of the dipion invariant-mass spectrum from the transition Υ(3S)-->Υ(1S)π0π0.

  11. Anatomy of new physics in B-B mixing

    SciTech Connect

    Lenz, A.; Nierste, U.; Charles, J.; Descotes-Genon, S.; Kaufhold, C.; T'Jampens, S.; Lacker, H.; Monteil, S.; Niess, V.

    2011-02-01

    We analyze three different new physics scenarios for {Delta}F=2 flavor-changing neutral currents in the quark sector in the light of recent data on neutral-meson mixing. We parametrize generic new physics contributions to B{sub q}-B{sub q} mixing, q=d, s, in terms of one complex quantity {Delta}{sub q}, while three parameters {Delta}{sub K}{sup tt}, {Delta}{sub K}{sup ct}, and {Delta}{sub K}{sup cc} are needed to describe K-K mixing. In scenario I, we consider uncorrelated new physics contributions in the B{sub d}, B{sub s}, and K sectors. In this scenario, it is only possible to constrain the parameters {Delta}{sub d} and {Delta}{sub s} whereas there are no nontrivial constraints on the kaon parameters. In scenario II, we study the case of minimal flavor violation (MFV) and small bottom Yukawa coupling, where {Delta}{identical_to}{Delta}{sub d}={Delta}{sub s}={Delta}{sub K}{sup tt}. We show that {Delta} must then be real, so that no new CP phases can be accommodated, and express the remaining parameters {Delta}{sub K}{sup cc} and {Delta}{sub K}{sup ct} in terms of {Delta} in this scenario. Scenario III is the generic MFV case with large bottom Yukawa couplings. In this case, the kaon sector is uncorrelated to the B{sub d} and B{sub s} sectors. As in the second scenario one has {Delta}{sub d}={Delta}{sub s{identical_to}{Delta}}, however, now with a complex parameter {Delta}. Our quantitative analyses consist of global Cabibbo-Kobayashi-Maskawa (CKM) fits within the Rfit frequentist statistical approach, determining the standard model parameters and the new physics parameters of the studied scenarios simultaneously. We find that the recent measurements indicating discrepancies with the standard model are well accommodated in Scenarios I and III with new mixing phases, with a slight preference for Scenario I that permits different new CP phases in the B{sub d} and B{sub s} systems. Within our statistical framework, we find evidence of new physics in both B{sub d} and B{sub s} systems. The standard model hypothesis {Delta}{sub d}={Delta}{sub s}=1 is disfavored with p-values of 3.6{sigma} and 3.3{sigma} in Scenarios I and III, respectively. We also present an exhaustive list of numerical predictions in each scenario. In particular, we predict the CP phase in B{sub s}{yields}J/{psi}{phi} and the difference between the B{sub s} and B{sub d} semileptonic asymmetries, which will be both measured by the LHCb experiment.

  12. Submillimeter line emission from LMC 30 Doradus: The impact of a starburst on a low-metallicity environment

    NASA Astrophysics Data System (ADS)

    Pineda, J. L.; Mizuno, N.; Röllig, M.; Stutzki, J.; Kramer, C.; Klein, U.; Rubio, M.; Kawamura, A.; Minamidani, T.; Benz, A.; Burton, M.; Fukui, Y.; Koo, B.-C.; Onishi, T.

    2012-08-01

    Context. The 30 Dor region in the Large Magellanic Cloud (LMC) is the most vigorous star-forming region in the Local Group. Star formation in this region is taking place in low-metallicity molecular gas that is exposed to an extreme far-ultraviolet (FUV) radiation field powered by the massive compact star cluster R136. 30 Dor is therefore ideally suited to study the conditions in which stars formed at earlier cosmological times. Aims: Observations of (sub)mm and far-infrared (FIR) spectral lines of the main carbon-carrying species, CO, [C i] and [C ii], which originate in the surface layers of molecular clouds illuminated by the FUV radiation of young stars, can be used to constrain the physical and chemical state of the star-forming ISM. Methods: We used the NANTEN2 telescope to obtain high-angular resolution observations of the 12CO J = 4 → 3, J = 7 → 6, and 13CO J = 4 → 3 rotational lines and [C i] 3P1- 3P0 and 3P2- 3P1 fine-structure submillimeter transitions in 30 Dor-10, the brightest CO and FIR-emitting cloud at the center of the 30 Dor region. We derived the physical and chemical properties of the low-metallicity molecular gas using an excitation/radiative transfer code and found a self-consistent solution of the chemistry and thermal balance of the gas in the framework of a clumpy cloud PDR model. We compared the derived properties with those in the N159W region, which is exposed to a more moderate far-ultraviolet radiation field compared with 30 Dor-10, but has similar metallicity. We also combined our CO detections with previously observed low-J CO transitions to derive the CO spectral-line energy distribution in 30 Dor-10 and N159W. Results: The separate excitation analysis of the submm CO lines and the neutral carbon fine structure lines shows that the mid-J CO and [C i]-emitting gas in the 30 Dor-10 region has a temperature of about 160 K and a H2 density of about 104 cm-3. We find that the molecular gas in 30 Dor-10 is warmer and has a lower beam filling factor compared to that of N159W, which might be a result of the effect of a strong FUV radiation field heating and disrupting the low-metallicity molecular gas. We use a clumpy PDR model (including the [C ii] line intensity reported in the literature) to constrain the FUV intensity to about χ0 ≈ 3100 and an average total H density of the clump ensemble of about 105 cm-3 in 30 Dor-10.

  13. The γ Doradus CoRoT target HD 49434. I. Results from the ground-based campaign

    NASA Astrophysics Data System (ADS)

    Uytterhoeven, K.; Mathias, P.; Poretti, E.; Rainer, M.; Martín-Ruiz, S.; Rodríguez, E.; Amado, P. J.; Le Contel, D.; Jankov, S.; Niemczura, E.; Pollard, K. R.; Brunsden, E.; Paparó, M.; Costa, V.; Valtier, J.-C.; Garrido, R.; Suárez, J. C.; Kilmartin, P. M.; Chapellier, E.; Rodríguez-López, C.; Marin, A. J.; Aceituno, F. J.; Casanova, V.; Rolland, A.; Olivares, I.

    2008-10-01

    Context: We present an extensive ground-based photometric and spectroscopic campaign of the γ Dor CoRoT target HD 49434. This campaign was a preparatory step of the CoRoT satellite observations, which occurred between October 2007 and March 2008. Aims: With satellite data, detection of low-degree pulsation modes only is achievable, and, as no filters are available, with poor identification. Ground-based data promise eventually to identify additional modes and provide extra input for the identification: spectroscopic data allows the detection of high-degree modes and an estimate of the azimuthal number m. We attempt to detect and identify as many pulsation modes as possible from the ground-based dataset of the γ Dor star HD 49434, and anticipate the CoRoT results. Methods: We searched for frequencies in the multi-colour variations, the pixel-to-pixel variations across the line profiles, and the moments variations in a large dataset, consisting of both multi-colour photometric and spectroscopic data from different observatories, using different frequency analysis methods. We performed a tentative mode identification of the spectroscopic frequencies using the Moment Method and the Intensity Period Search Method. We also completed an abundance analysis. Results: The frequency analysis clearly indicates the presence of four frequencies in the 0.2-1.7 d-1 interval, as well as six frequencies in the 5-12 d-1 domain. The low frequencies are typical of γ Dor variables, while the high frequencies are common to δ Sct pulsators. We propose that the frequency 2.666 d-1 is the rotational frequency. All modes, for which an identification was possible, appear to be high-degree modes (3 ≤ ell ≤ 8). We did not find evidence for a possible binary nature of the star HD 49434. The element abundances that we derived are consistent with values obtained in previous analyses. Conclusions: We classify the γ Dor star HD 49434 as a hybrid pulsator, which pulsates simultaneously in p- and g-modes. This implies that HD 49434 is an extremely interesting target for asteroseismic modelling. Based on observations made with ESO Telescopes at the La Silla Observatory under the ESO Large Programme: LP178.D-0361, and on data collected at the Centro Astronómico Hispano Alemán (CAHA) at Calar Alto, operated jointly by the Max-Planck Institut für Astronomie and the Instituto de Astrofísica de Andalucía (CSIC). Also based on observations obtained at Observatorio de Sierra Nevada (Spain), at Observatorio Astronómico Nacional San Pedro Mártir (Mexico), at the Piszkéstetõ Mountain Station of Konkoly Observatory (Hungary), at Observatoire de Haute Provence (France) and at Mount John University Observatory (New Zealand). Tables [see full textsee full textsee full text], [see full textsee full textsee full text] and [see full textsee full textsee full text] are only available in electronic form at http://www.aanda.org