LINC Complexes Form by Binding of Three KASH Peptides to Domain Interfaces of Trimeric SUN Proteins

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sosa, Brian A.; Rothballer, Andrea; Kutay, Ulrike

Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope and are composed of KASH and SUN proteins residing in the outer and inner nuclear membrane, respectively. LINC formation relies on direct binding of KASH and SUN in the perinuclear space. Thereby, molecular tethers are formed that can transmit forces for chromosome movements, nuclear migration, and anchorage. We present crystal structures of the human SUN2-KASH1/2 complex, the core of the LINC complex. The SUN2 domain is rigidly attached to a trimeric coiled coil that prepositions it to bind three KASH peptides. The peptides bind in three deep and expansivemore » grooves formed between adjacent SUN domains, effectively acting as molecular glue. In addition, a disulfide between conserved cysteines on SUN and KASH covalently links both proteins. The structure provides the basis of LINC complex formation and suggests a model for how LINC complexes might arrange into higher-order clusters to enhance force-coupling.« less

Perispeckles are major assembly sites for the exon junction core complex

PubMed Central

Daguenet, Elisabeth; Baguet, Aurélie; Degot, Sébastien; Schmidt, Ute; Alpy, Fabien; Wendling, Corinne; Spiegelhalter, Coralie; Kessler, Pascal; Rio, Marie-Christine; Le Hir, Hervé; Bertrand, Edouard; Tomasetto, Catherine

2012-01-01

The exon junction complex (EJC) is loaded onto mRNAs as a consequence of splicing and regulates multiple posttranscriptional events. MLN51, Magoh, Y14, and eIF4A3 form a highly stable EJC core, but where this tetrameric complex is assembled in the cell remains unclear. Here we show that EJC factors are enriched in domains that we term perispeckles and are visible as doughnuts around nuclear speckles. Fluorescence resonance energy transfer analyses and EJC assembly mutants show that perispeckles do not store free subunits, but instead are enriched for assembled cores. At the ultrastructural level, perispeckles are distinct from interchromatin granule clusters that may function as storage sites for splicing factors and intermingle with perichromatin fibrils, where nascent RNAs and active RNA Pol II are present. These results support a model in which perispeckles are major assembly sites for the tetrameric EJC core. This subnuclear territory thus represents an intermediate region important for mRNA maturation, between transcription sites and splicing factor reservoirs and assembly sites. PMID:22419818

Nup93, a Vertebrate Homologue of Yeast Nic96p, Forms a Complex with a Novel 205-kDa Protein and Is Required for Correct Nuclear Pore Assembly

PubMed Central

Grandi, Paola; Dang, Tam; Pané, Nelly; Shevchenko, Andrej; Mann, Matthias; Forbes, Douglass; Hurt, Ed

1997-01-01

Yeast and vertebrate nuclear pores display significant morphological similarity by electron microscopy, but sequence similarity between the respective proteins has been more difficult to observe. Herein we have identified a vertebrate nucleoporin, Nup93, in both human and Xenopus that has proved to be an evolutionarily related homologue of the yeast nucleoporin Nic96p. Polyclonal antiserum to human Nup93 detects corresponding proteins in human, rat, and Xenopus cells. Immunofluorescence and immunoelectron microscopy localize vertebrate Nup93 at the nuclear basket and at or near the nuclear entry to the gated channel of the pore. Immunoprecipitation from both mammalian and Xenopus cell extracts indicates that a small fraction of Nup93 physically interacts with the nucleoporin p62, just as yeast Nic96p interacts with the yeast p62 homologue. However, a large fraction of vertebrate Nup93 is extracted from pores and is also present in Xenopus egg extracts in complex with a newly discovered 205-kDa protein. Mass spectrometric sequencing of the human 205-kDa protein reveals that this protein is encoded by an open reading frame, KIAAO225, present in the human database. The putative human nucleoporin of 205 kDa has related sequence homologues in Caenorhabditis elegans and Saccharomyces cerevisiae. To analyze the role of the Nup93 complex in the pore, nuclei were assembled that lack the Nup93 complex after immunodepletion of a Xenopus nuclear reconstitution extract. The Nup93-complex–depleted nuclei are clearly defective for correct nuclear pore assembly. From these experiments, we conclude that the vertebrate and yeast pore have significant homology in their functionally important cores and that, with the identification of Nup93 and the 205-kDa protein, we have extended the knowledge of the nearest-neighbor interactions of this core in both yeast and vertebrates. PMID:9348540

Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.

PubMed

Cevher, Murat A; Shi, Yi; Li, Dan; Chait, Brian T; Malik, Sohail; Roeder, Robert G

2014-12-01

The evolutionarily conserved Mediator complex is a critical coactivator for RNA polymerase II (Pol II)-mediated transcription. Here we report the reconstitution of a functional 15-subunit human core Mediator complex and its characterization by functional assays and chemical cross-linking coupled to MS (CX-MS). Whereas the reconstituted head and middle modules can stably associate, basal and coactivator functions are acquired only after incorporation of MED14 into the bimodular complex. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. Altogether, our analyses identify MED14 as both an architectural and a functional backbone of the Mediator complex. We further establish a conditional requirement for metazoan-specific MED26 that becomes evident in the presence of heterologous nuclear factors. This general approach paves the way for systematic dissection of the multiple layers of functionality associated with the Mediator complex.

Mediator-dependent Nuclear Receptor Functions

PubMed Central

Chen, Wei; Roeder, Robert

2011-01-01

As gene-specific transcription factors, nuclear hormone receptors are broadly involved in many important biological processes. Their function on target genes requires the stepwise assembly of different coactivator complexes that facilitate chromatin remodeling and subsequent preinitiation complex (PIC) formation and function. Mediator has proved to be a crucial, and general, nuclear receptor-interacting coactivator, with demonstrated functions in transcription steps ranging from chromatin remodeling to subsequent PIC formation and function. Here we discuss (i) our current understanding of pathways that nuclear receptors and other interacting cofactors employ to recruit Mediator to target gene enhancers and promoters, including conditional requirements for the strong NR-Mediator interactions mediated by the NR AF2 domain and the MED1 LXXLLL motifs and (ii) mechanisms by which Mediator acts to transmit signals from enhancer-bound nuclear receptors to the general transcription machinery at core promoters to effect PIC formation and function. PMID:21854863

Repair pathways independent of the Fanconi anemia nuclear core complex play a predominant role in mitigating formaldehyde-induced DNA damage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Noda, Taichi; Department of Dermatology, School of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521; Takahashi, Akihisa

2011-01-07

The role of the Fanconi anemia (FA) repair pathway for DNA damage induced by formaldehyde was examined in the work described here. The following cell types were used: mouse embryonic fibroblast cell lines FANCA{sup -/-}, FANCC{sup -/-}, FANCA{sup -/-}C{sup -/-}, FANCD2{sup -/-} and their parental cells, the Chinese hamster cell lines FANCD1 mutant (mt), FANCGmt, their revertant cells, and the corresponding wild-type (wt) cells. Cell survival rates were determined with colony formation assays after formaldehyde treatment. DNA double strand breaks (DSBs) were detected with an immunocytochemical {gamma}H2AX-staining assay. Although the sensitivity of FANCA{sup -/-}, FANCC{sup -/-} and FANCA{sup -/-}C{sup -/-}more » cells to formaldehyde was comparable to that of proficient cells, FANCD1mt, FANCGmt and FANCD2{sup -/-} cells were more sensitive to formaldehyde than the corresponding proficient cells. It was found that homologous recombination (HR) repair was induced by formaldehyde. In addition, {gamma}H2AX foci in FANCD1mt cells persisted for longer times than in FANCD1wt cells. These findings suggest that formaldehyde-induced DSBs are repaired by HR through the FA repair pathway which is independent of the FA nuclear core complex. -- Research highlights: {yields} We examined to clarify the repair pathways of formaldehyde-induced DNA damage. Formaldehyde induces DNA double strand breaks (DSBs). {yields} DSBs are repaired through the Fanconi anemia (FA) repair pathway. {yields} This pathway is independent of the FA nuclear core complex. {yields} We also found that homologous recombination repair was induced by formaldehyde.« less

Beyond tRNA cleavage: novel essential function for yeast tRNA splicing endonuclease unrelated to tRNA processing

PubMed Central

Dhungel, Nripesh; Hopper, Anita K.

2012-01-01

Pre-tRNA splicing is an essential process in all eukaryotes. In yeast and vertebrates, the enzyme catalyzing intron removal from pre-tRNA is a heterotetrameric complex (splicing endonuclease [SEN] complex). Although the SEN complex is conserved, the subcellular location where pre-tRNA splicing occurs is not. In yeast, the SEN complex is located at the cytoplasmic surface of mitochondria, whereas in vertebrates, pre-tRNA splicing is nuclear. We engineered yeast to mimic the vertebrate cell biology and demonstrate that all three steps of pre-tRNA splicing, as well as tRNA nuclear export and aminoacylation, occur efficiently when the SEN complex is nuclear. However, nuclear pre-tRNA splicing fails to complement growth defects of cells with defective mitochondrial-located splicing, suggesting that the yeast SEN complex surprisingly serves a novel and essential function in the cytoplasm that is unrelated to tRNA splicing. The novel function requires all four SEN complex subunits and the catalytic core. A subset of pre-rRNAs accumulates when the SEN complex is restricted to the nucleus, indicating that the SEN complex moonlights in rRNA processing. Thus, findings suggest that selection for the subcellular distribution of the SEN complex may reside not in its canonical, but rather in a novel, activity. PMID:22391451

Nuclear thermal propulsion test facility requirements and development strategy

NASA Technical Reports Server (NTRS)

Allen, George C.; Warren, John; Clark, J. S.

1991-01-01

The Nuclear Thermal Propulsion (NTP) subpanel of the Space Nuclear Propulsion Test Facilities Panel evaluated facility requirements and strategies for nuclear thermal propulsion systems development. High pressure, solid core concepts were considered as the baseline for the evaluation, with low pressure concepts an alternative. The work of the NTP subpanel revealed that a wealth of facilities already exists to support NTP development, and that only a few new facilities must be constructed. Some modifications to existing facilities will be required. Present funding emphasis should be on long-lead-time items for the major new ground test facility complex and on facilities supporting nuclear fuel development, hot hydrogen flow test facilities, and low power critical facilities.

The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression

PubMed Central

Schneider, Maren; Hellerschmied, Doris; Schubert, Tobias; Amlacher, Stefan; Vinayachandran, Vinesh; Reja, Rohit; Pugh, B. Franklin; Clausen, Tim; Köhler, Alwin

2015-01-01

Summary Nuclear pore complexes (NPCs) influence gene expression besides their established function in nuclear transport. The TREX-2 complex localizes to the NPC basket and affects gene-NPC interactions, transcription, and mRNA export. How TREX-2 regulates the gene expression machinery is unknown. Here, we show that TREX-2 interacts with the Mediator complex, an essential regulator of RNA Polymerase (Pol) II. Structural and biochemical studies identify a conserved region on TREX-2, which directly binds the Mediator Med31/Med7N submodule. TREX-2 regulates assembly of Mediator with the Cdk8 kinase and is required for recruitment and site-specific phosphorylation of Pol II. Transcriptome and phenotypic profiling confirm that TREX-2 and Med31 are functionally interdependent at specific genes. TREX-2 additionally uses its Mediator-interacting surface to regulate mRNA export suggesting a mechanism for coupling transcription initiation and early steps of mRNA processing. Our data provide mechanistic insight into how an NPC-associated adaptor complex accesses the core transcription machinery. PMID:26317468

Flexible Stoichiometry and Asymmetry of the PIDDosome Core Complex by Heteronuclear NMR Spectroscopy and Mass Spectrometry

PubMed Central

Nematollahi, Lily A.; Garza-Garcia, Acely; Bechara, Chérine; Esposito, Diego; Morgner, Nina; Robinson, Carol V.; Driscoll, Paul C.

2015-01-01

Homotypic death domain (DD)–DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing five PIDD-DDs in one layer, five RAIDD-DDs in a second layer and an additional two RAIDD-DDs. We addressed complex formation between PIDD-DD and RAIDD-DD in solution using heteronuclear nuclear magnetic resonance (NMR) spectroscopy, nanoflow electrospray ionization mass spectrometry and size-exclusion chromatography with multi-angle light scattering. The DDs assemble into complexes displaying molecular masses in the range 130–158 kDa and RAIDD-DD:PIDD-DD stoichiometries of 5:5, 6:5 and 7:5. These data suggest that the crystal structure is representative of only the heaviest species in solution and that two RAIDD-DDs are loosely attached to the 5:5 core. Two-dimensional 1H,15N-NMR experiments exhibited signal loss upon complexation consistent with the formation of high-molecular-weight species. 13C-Methyl-transverse relaxation optimized spectroscopy measurements of the PIDDosome core exhibit signs of differential line broadening, cross-peak splitting and chemical shift heterogeneity that reflect the presence of non-equivalent sites at interfaces within an asymmetric complex. Experiments using a mutant RAIDD-DD that forms a monodisperse 5:5 complex with PIDD-DD show that the spectroscopic signature derives from the quasi- but non-exact equivalent environments of each DD. Since this characteristic was previously demonstrated for the complex between the DDs of CD95 and FADD, the NMR data for this system are consistent with the formation of a structure homologous to the PIDDosome core. PMID:25528640

"What--me worry?" "Why so serious?": a personal view on the Fukushima nuclear reactor accidents.

PubMed

Gallucci, Raymond

2012-09-01

Infrequently, it seems that a significant accident precursor or, worse, an actual accident, involving a commercial nuclear power reactor occurs to remind us of the need to reexamine the safety of this important electrical power technology from a risk perspective. Twenty-five years since the major core damage accident at Chernobyl in the Ukraine, the Fukushima reactor complex in Japan experienced multiple core damages as a result of an earthquake-induced tsunami beyond either the earthquake or tsunami design basis for the site. Although the tsunami itself killed tens of thousands of people and left the area devastated and virtually uninhabitable, much concern still arose from the potential radioactive releases from the damaged reactors, even though there was little population left in the area to be affected. As a lifelong probabilistic safety analyst in nuclear engineering, even I must admit to a recurrence of the doubt regarding nuclear power safety after Fukushima that I had experienced after Three Mile Island and Chernobyl. This article is my attempt to "recover" my personal perspective on acceptable risk by examining both the domestic and worldwide history of commercial nuclear power plant accidents and attempting to quantify the risk in terms of the frequency of core damage that one might glean from a review of operational history. © 2012 Society for Risk Analysis.

Reconstitution of active human core Mediator complex reveals a pivotal role of the MED14 subunit

PubMed Central

Cevher, Murat A.; Shi, Yi; Li, Dan; Chait, Brian T.; Malik, Sohail; Roeder, Robert G.

2014-01-01

The evolutionarily conserved Mediator complex is a critical coactivator for RNA polymerase II (Pol II)-mediated transcription. Here, we report the reconstitution of a functional 15-subunit human core Mediator complex and its characterization by functional assays and chemical cross-linking coupled to mass spectrometry (CX-MS). Whereas the reconstituted head and middle modules can stably associate, only with incorporation of MED14 into the bi-modular complex does it acquire basal and coactivator functions. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. Altogether, our analyses identify MED14 as both an architectural and a functional backbone of the Mediator complex. We further establish a conditional requirement for metazoan-specific MED26 that becomes evident in the presence of heterologous nuclear factors. This general approach paves the way for systematically dissecting the multiple layers of functionalities associated with the Mediator complex. PMID:25383669

AMP-activated protein kinase is involved in the activation of the Fanconi anemia/BRCA pathway in response to DNA interstrand crosslinks.

PubMed

Chun, Min Jeong; Kim, Sunshin; Hwang, Soo Kyung; Kim, Bong Sub; Kim, Hyoun Geun; Choi, Hae In; Kim, Jong Heon; Goh, Sung Ho; Lee, Chang-Hun

2016-08-16

Fanconi anemia complementation group (FANC) proteins constitute the Fanconi Anemia (FA)/BRCA pathway that is activated in response to DNA interstrand crosslinks (ICLs). We previously performed yeast two-hybrid screening to identify novel FANC-interacting proteins and discovered that the alpha subunit of AMP-activated protein kinase (AMPKα1) was a candidate binding partner of the FANCG protein, which is a component of the FA nuclear core complex. We confirmed the interaction between AMPKα and both FANCG using co-immunoprecipitation experiments. Additionally, we showed that AMPKα interacted with FANCA, another component of the FA nuclear core complex. AMPKα knockdown in U2OS cells decreased FANCD2 monoubiquitination and nuclear foci formation upon mitomycin C-induced ICLs. Furthermore, AMPKα knockdown enhanced cellular sensitivity to MMC. MMC treatment resulted in an increase in AMPKα phosphorylation/activation, indicating AMPK is involved in the cellular response to ICLs. FANCA was phosphorylated by AMPK at S347 and phosphorylation increased with MMC treatment. MMC-induced FANCD2 monoubiquitination and nuclear foci formation were compromised in a U2OS cell line that stably overexpressed the S347A mutant form of FANCA compared to wild-type FANCA-overexpressing cells, indicating a requirement for FANCA phosphorylation at S347 for proper activation of the FA/BRCA pathway. Our data suggest AMPK is involved in the activation of the FA/BRCA pathway.

Nuclear Thermal Propulsion: A Joint NASA/DOE/DOD Workshop

NASA Technical Reports Server (NTRS)

Clark, John S. (Editor)

1991-01-01

Papers presented at the joint NASA/DOE/DOD workshop on nuclear thermal propulsion are compiled. The following subject areas are covered: nuclear thermal propulsion programs; Rover/NERVA and NERVA systems; Low Pressure Nuclear Thermal Rocket (LPNTR); particle bed reactor nuclear rocket; hybrid propulsion systems; wire core reactor; pellet bed reactor; foil reactor; Droplet Core Nuclear Rocket (DCNR); open cycle gas core nuclear rockets; vapor core propulsion reactors; nuclear light bulb; Nuclear rocket using Indigenous Martian Fuel (NIMF); mission analysis; propulsion and reactor technology; development plans; and safety issues.

Structure and reconstitution of yeast Mpp6-nuclear exosome complexes reveals that Mpp6 stimulates RNA decay and recruits the Mtr4 helicase.

PubMed

Wasmuth, Elizabeth V; Zinder, John C; Zattas, Dimitrios; Das, Mom; Lima, Christopher D

2017-07-25

Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase. Mpp6 interacts with the nine-subunit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 and recruits Mtr4, but it is less clear if these cofactors work together. Using biochemistry with Saccharomyces cerevisiae proteins, we show that Rrp47 and Mpp6 stimulate exosome-mediated RNA decay, albeit with unique dependencies on elements within the nuclear exosome. Mpp6-exosomes can recruit Mtr4, while Mpp6 and Rrp47 each contribute to Mtr4-dependent RNA decay, with maximal Mtr4-dependent decay observed with both cofactors. The 3.3 Å structure of a twelve-subunit nuclear Mpp6 exosome bound to RNA shows the central region of Mpp6 bound to the exosome core, positioning its Mtr4 recruitment domain next to Rrp6 and the exosome central channel. Genetic analysis reveals interactions that are largely consistent with our model.

Nuclear reactor apparatus

DOEpatents

Wade, Elman E.

1978-01-01

A lifting, rotating and sealing apparatus for nuclear reactors utilizing rotating plugs above the nuclear reactor core. This apparatus permits rotation of the plugs to provide under the plug refueling of a nuclear core. It also provides a means by which positive top core holddown can be utilized. Both of these operations are accomplished by means of the apparatus lifting the top core holddown structure off the nuclear core while stationary, and maintaining this structure in its elevated position during plug rotation. During both of these operations, the interface between the rotating member and its supporting member is sealingly maintained.

Mitotic phosphorylation of SUN1 loosens its connection with the nuclear lamina while the LINC complex remains intact.

PubMed

Patel, Jennifer T; Bottrill, Andrew; Prosser, Suzanna L; Jayaraman, Sangeetha; Straatman, Kees; Fry, Andrew M; Shackleton, Sue

2014-01-01

At the onset mitosis in higher eukaryotes, the nuclear envelope (NE) undergoes dramatic deconstruction to allow separation of duplicated chromosomes. Studies have shown that during this process of nuclear envelope breakdown (NEBD), the extensive protein networks of the nuclear lamina are disassembled through phosphorylation of lamins and several inner nuclear membrane (INM) proteins. The LINC complex, composed of SUN and nesprin proteins, is involved in multiple interactions at the NE and plays vital roles in nuclear and cellular mechanics by connecting the nucleus to the cytoskeleton. Here, we show that SUN1, located in the INM, undergoes mitosis-specific phosphorylation on at least 3 sites within its nucleoplasmic N-terminus. We further identify Cdk1 as the kinase responsible for serine 48 and 333 phosphorylation, while serine 138 is phosphorylated by Plk1. In mitotic cells, SUN1 loses its interaction with N-terminal domain binding partners lamin A/C, emerin, and short nesprin-2 isoforms. Furthermore, a triple phosphomimetic SUN1 mutant displays increased solubility and reduced retention at the NE. In contrast, the central LINC complex interaction between the SUN1 C-terminus and the KASH domain of nesprin-2 is maintained during mitosis. Together, these data support a model whereby mitotic phosphorylation of SUN1 disrupts interactions with nucleoplasmic binding partners, promoting disassembly of the nuclear lamina and, potentially, its chromatin interactions. At the same time, our data add to an emerging picture that the core LINC complex plays an active role in NEBD.

77 FR 30435 - In-core Thermocouples at Different Elevations and Radial Positions in Reactor Core

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-23

... NUCLEAR REGULATORY COMMISSION 10 CFR Part 50 [Docket No. PRM-50-105; NRC-2012-0056] In-core Thermocouples at Different Elevations and Radial Positions in Reactor Core AGENCY: Nuclear Regulatory Commission... of operating licenses for nuclear power plants (``NPP'') to operate NPPs with in-core thermocouples...

Dynamic nuclear polarization assisted spin diffusion for the solid effect case.

PubMed

Hovav, Yonatan; Feintuch, Akiva; Vega, Shimon

2011-02-21

The dynamic nuclear polarization (DNP) process in solids depends on the magnitudes of hyperfine interactions between unpaired electrons and their neighboring (core) nuclei, and on the dipole-dipole interactions between all nuclei in the sample. The polarization enhancement of the bulk nuclei has been typically described in terms of a hyperfine-assisted polarization of a core nucleus by microwave irradiation followed by a dipolar-assisted spin diffusion process in the core-bulk nuclear system. This work presents a theoretical approach for the study of this combined process using a density matrix formalism. In particular, solid effect DNP on a single electron coupled to a nuclear spin system is considered, taking into account the interactions between the spins as well as the main relaxation mechanisms introduced via the electron, nuclear, and cross-relaxation rates. The basic principles of the DNP-assisted spin diffusion mechanism, polarizing the bulk nuclei, are presented, and it is shown that the polarization of the core nuclei and the spin diffusion process should not be treated separately. To emphasize this observation the coherent mechanism driving the pure spin diffusion process is also discussed. In order to demonstrate the effects of the interactions and relaxation mechanisms on the enhancement of the nuclear polarization, model systems of up to ten spins are considered and polarization buildup curves are simulated. A linear chain of spins consisting of a single electron coupled to a core nucleus, which in turn is dipolar coupled to a chain of bulk nuclei, is considered. The interaction and relaxation parameters of this model system were chosen in a way to enable a critical analysis of the polarization enhancement of all nuclei, and are not far from the values of (13)C nuclei in frozen (glassy) organic solutions containing radicals, typically used in DNP at high fields. Results from the simulations are shown, demonstrating the complex dependences of the DNP-assisted spin diffusion process on variations of the relevant parameters. In particular, the effect of the spin lattice relaxation times on the polarization buildup times and the resulting end polarization are discussed, and the quenching of the polarizations by the hyperfine interaction is demonstrated.

Multiple TPR motifs characterize the Fanconi anemia FANCG protein.

PubMed

Blom, Eric; van de Vrugt, Henri J; de Vries, Yne; de Winter, Johan P; Arwert, Fré; Joenje, Hans

2004-01-05

The genome protection pathway that is defective in patients with Fanconi anemia (FA) is controlled by at least eight genes, including BRCA2. A key step in the pathway involves the monoubiquitylation of FANCD2, which critically depends on a multi-subunit nuclear 'core complex' of at least six FANC proteins (FANCA, -C, -E, -F, -G, and -L). Except for FANCL, which has WD40 repeats and a RING finger domain, no significant domain structure has so far been recognized in any of the core complex proteins. By using a homology search strategy comparing the human FANCG protein sequence with its ortholog sequences in Oryzias latipes (Japanese rice fish) and Danio rerio (zebrafish) we identified at least seven tetratricopeptide repeat motifs (TPRs) covering a major part of this protein. TPRs are degenerate 34-amino acid repeat motifs which function as scaffolds mediating protein-protein interactions, often found in multiprotein complexes. In four out of five TPR motifs tested (TPR1, -2, -5, and -6), targeted missense mutagenesis disrupting the motifs at the critical position 8 of each TPR caused complete or partial loss of FANCG function. Loss of function was evident from failure of the mutant proteins to complement the cellular FA phenotype in FA-G lymphoblasts, which was correlated with loss of binding to FANCA. Although the TPR4 mutant fully complemented the cells, it showed a reduced interaction with FANCA, suggesting that this TPR may also be of functional importance. The recognition of FANCG as a typical TPR protein predicts this protein to play a key role in the assembly and/or stabilization of the nuclear FA protein core complex.

AMP-activated protein kinase is involved in the activation of the Fanconi anemia/BRCA pathway in response to DNA interstrand crosslinks

PubMed Central

Hwang, Soo Kyung; Kim, Bong Sub; Kim, Hyoun Geun; Choi, Hae In; Kim, Jong Heon; Goh, Sung Ho; Lee, Chang-Hun

2016-01-01

Fanconi anemia complementation group (FANC) proteins constitute the Fanconi Anemia (FA)/BRCA pathway that is activated in response to DNA interstrand crosslinks (ICLs). We previously performed yeast two-hybrid screening to identify novel FANC-interacting proteins and discovered that the alpha subunit of AMP-activated protein kinase (AMPKα1) was a candidate binding partner of the FANCG protein, which is a component of the FA nuclear core complex. We confirmed the interaction between AMPKα and both FANCG using co-immunoprecipitation experiments. Additionally, we showed that AMPKα interacted with FANCA, another component of the FA nuclear core complex. AMPKα knockdown in U2OS cells decreased FANCD2 monoubiquitination and nuclear foci formation upon mitomycin C-induced ICLs. Furthermore, AMPKα knockdown enhanced cellular sensitivity to MMC. MMC treatment resulted in an increase in AMPKα phosphorylation/activation, indicating AMPK is involved in the cellular response to ICLs. FANCA was phosphorylated by AMPK at S347 and phosphorylation increased with MMC treatment. MMC-induced FANCD2 monoubiquitination and nuclear foci formation were compromised in a U2OS cell line that stably overexpressed the S347A mutant form of FANCA compared to wild-type FANCA-overexpressing cells, indicating a requirement for FANCA phosphorylation at S347 for proper activation of the FA/BRCA pathway. Our data suggest AMPK is involved in the activation of the FA/BRCA pathway. PMID:27449087

Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane

PubMed Central

Mitchell, Jana M.; Mansfeld, Jörg; Capitanio, Juliana; Kutay, Ulrike

2010-01-01

Nuclear pore complexes (NPCs) control the movement of molecules across the nuclear envelope (NE). We investigated the molecular interactions that exist at the interface between the NPC scaffold and the pore membrane. We show that key players mediating these interactions in mammalian cells are the nucleoporins Nup155 and Nup160. Nup155 depletion massively alters NE structure, causing a dramatic decrease in NPC numbers and the improper targeting of membrane proteins to the inner nuclear membrane. The role of Nup155 in assembly is likely closely linked to events at the membrane as we show that Nup155 interacts with pore membrane proteins Pom121 and NDC1. Furthermore, we demonstrate that the N terminus of Pom121 directly binds the β-propeller regions of Nup155 and Nup160. We propose a model in which the interactions of Pom121 with Nup155 and Nup160 are predicted to assist in the formation of the nuclear pore and the anchoring of the NPC to the pore membrane. PMID:20974814

Structure of a Yeast Dyn2-Nup159 Complex and Molecular Basis for Dynein Light Chain-Nuclear Pore Interaction*

PubMed Central

Romes, Erin M.; Tripathy, Ashutosh; Slep, Kevin C.

2012-01-01

The nuclear pore complex gates nucleocytoplasmic transport through a massive, eight-fold symmetric channel capped by a nucleoplasmic basket and structurally unique, cytoplasmic fibrils whose tentacles bind and regulate asymmetric traffic. The conserved Nup82 complex, composed of Nsp1, Nup82, and Nup159, forms the unique cytoplasmic fibrils that regulate mRNA nuclear export. Although the nuclear pore complex plays a fundamental, conserved role in nuclear trafficking, structural information about the cytoplasmic fibrils is limited. Here, we investigate the structural and biochemical interactions between Saccharomyces cerevisiae Nup159 and the nucleoporin, Dyn2. We find that Dyn2 is predominantly a homodimer and binds arrayed sites on Nup159, promoting the Nup159 parallel homodimerization. We present the first structure of Dyn2, determined at 1.85 Å resolution, complexed with a Nup159 target peptide. Dyn2 resembles homologous metazoan dynein light chains, forming homodimeric composite substrate binding sites that engage two independent 10-residue target motifs, imparting a β-strand structure to each peptide via antiparallel extension of the Dyn2 core β-sandwich. Dyn2 recognizes a highly conserved QT motif while allowing sequence plasticity in the flanking residues of the peptide. Isothermal titration calorimetric analysis of the comparative binding of Dyn2 to two Nup159 target sites shows similar affinities (18 and 13 μm), but divergent thermal binding modes. Dyn2 homodimers are arrayed in the crystal lattice, likely mimicking the arrayed architecture of Dyn2 on the Nup159 multivalent binding sites. Crystallographic interdimer interactions potentially reflect a cooperative basis for Dyn2-Nup159 complex formation. Our data highlight the determinants that mediate oligomerization of the Nup82 complex and promote a directed, elongated cytoplasmic fibril architecture. PMID:22411995

Transcription factor FoxA (HNF3) on a nucleosome at an enhancer complex in liver chromatin.

PubMed

Chaya, D; Hayamizu, T; Bustin, M; Zaret, K S

2001-11-30

Nucleosome-like particles and acetylated histones occur near active promoters and enhancers, and certain transcription factors can recognize their target sites on the surface of a nucleosome in vitro; yet it has been unclear whether transcription factors can occupy target sites on nucleosomes in native chromatin. We developed a method for sequential chromatin immunoprecipitation of distinct nuclear proteins that are simultaneously cross-linked to nucleosome-sized genomic DNA segments. We find that core histone H2A co-occupies, along with the FoxA (hepatocyte nuclear factor-3) transcription factor, DNA for the albumin transcriptional enhancer in native liver chromatin, where the enhancer is active. Because histone H2A on nuclear DNA is only known to exist in nucleosomes, we conclude that transcription factors can form a stable complex on nucleosomes at an active enhancer element in vivo.

Impact of thermal spectrum small modular reactors on performance of once-through nuclear fuel cycles with low-enriched uranium

DOE PAGES

Brown, Nicholas R.; Worrall, Andrew; Todosow, Michael

2016-11-18

Small modular reactors (SMRs) offer potential benefits, such as enhanced operational flexibility. However, it is vital to understand the holistic impact of SMRs on nuclear fuel cycle performance. The focus of this paper is the fuel cycle impacts of light water SMRs in a once-through fuel cycle with low-enriched uranium fuel. A key objective of this paper is to describe preliminary example reactor core physics and fuel cycle analyses conducted in support of the U.S. Department of Energy, Office of Nuclear Energy, Fuel Cycle Options Campaign. The hypothetical light water SMR example case considered in these preliminary scoping studies ismore » a cartridge type one-batch core with slightly less than 5.0% enrichment. Challenges associated with SMRs include increased neutron leakage, fewer assemblies in the core (and therefore fewer degrees of freedom in the core design), complex enrichment and burnable absorber loadings, full power operation with inserted control rods, the potential for frequent load-following operation, and shortened core height. Each of these will impact the achievable discharge burnup in the reactor and the fuel cycle performance. This paper summarizes a list of the factors relevant to SMR fuel, core, and operation that will impact fuel cycle performance. The high-level issues identified and preliminary scoping calculations in this paper are intended to inform on potential fuel cycle impacts of one-batch thermal spectrum SMRs. In particular, this paper highlights the impact of increased neutron leakage and reduced number of batches on the achievable burnup of the reactor. Fuel cycle performance metrics for a hypothetical example SMR are compared with those for a conventional three-batch light water reactor in the following areas: nuclear waste management, environmental impact, and resource utilization. The metrics performance for such an SMR is degraded for the mass of spent nuclear fuel and high-level waste disposed of, mass of depleted uranium disposed of, land use per energy generated, and carbon emissions per energy generated. Finally, it is noted that the features of some SMR designs impact three main aspects of fuel cycle performance: (1) small cores which means high leakage (there is a radial and axial component), (2) no boron which means heterogeneous core and extensive use of control rods and BPs, and (3) single batch cores. But not all of the SMR designs have all of these traits. As a result, the approach used in this study is therefore a bounding case and not all SMRs may be affected to the same extent.« less

The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly

PubMed Central

Makio, Tadashi; Stanton, Leslie H.; Lin, Cheng-Chao; Goldfarb, David S.; Weis, Karsten

2009-01-01

We have established that two homologous nucleoporins, Nup170p and Nup157p, play an essential role in the formation of nuclear pore complexes (NPCs) in Saccharomyces cerevisiae. By regulating their synthesis, we showed that the loss of these nucleoporins triggers a decrease in NPCs caused by a halt in new NPC assembly. Preexisting NPCs are ultimately lost by dilution as cells grow, causing the inhibition of nuclear transport and the loss of viability. Significantly, the loss of Nup170p/Nup157p had distinct effects on the assembly of different architectural components of the NPC. Nucleoporins (nups) positioned on the cytoplasmic face of the NPC rapidly accumulated in cytoplasmic foci. These nup complexes could be recruited into new NPCs after reinitiation of Nup170p synthesis, and may represent a physiological intermediate. Loss of Nup170p/Nup157p also caused core and nucleoplasmically positioned nups to accumulate in NPC-like structures adjacent to the inner nuclear membrane, which suggests that these nucleoporins are required for formation of the pore membrane and the incorporation of cytoplasmic nups into forming NPCs. PMID:19414608

Nuclear pasta in protoneutron stars: simulations of neutrino emission from nucelar de-excitation

NASA Astrophysics Data System (ADS)

Witt, Matthew Charles; Newton, William

2017-01-01

Nuclear pasta is an exotic phase of matter with densities near ρ ≈ ρ0 = 1014 g cm-3 that consists of complex structures with geometries resembling spaghetti, lasagna, gnocchi, and other types of pasta. It is predicted to appear in the inner crust of neutron stars, protoneutron stars, and the collapsing cores of massive stars. It is hypothesized that nuclear pasta has a significant effect on transport and neutrino scattering properties of neutron and protoneutron stars. If this is true, then it is possible to find observational signatures of nuclear pasta. We present a calculation of neutrino emmissivity of pasta phases due to de-excitation of neutrons. We discuss observational implications on the neutrino signal of protoneutron stars.

Assessment of the Neutronic and Fuel Cycle Performance of the Transatomic Power Molten Salt Reactor Design

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robertson, Sean; Dewan, Leslie; Massie, Mark

This report presents results from a collaboration between Transatomic Power Corporation (TAP) and Oak Ridge National Laboratory (ORNL) to provide neutronic and fuel cycle analysis of the TAP core design through the Department of Energy Gateway for Accelerated Innovation in Nuclear (GAIN) Nuclear Energy Voucher program. The TAP concept is a molten salt reactor using configurable zirconium hydride moderator rod assemblies to shift the neutron spectrum in the core from mostly epithermal at beginning of life to thermal at end of life. Additional developments in the ChemTriton modeling and simulation tool provide the critical moderator-to-fuel ratio searches and time-dependent parametersmore » necessary to simulate the continuously changing physics in this complex system. The implementation of continuous-energy Monte Carlo transport and depletion tools in ChemTriton provide for full-core three-dimensional modeling and simulation. Results from simulations with these tools show agreement with TAP-calculated performance metrics for core lifetime, discharge burnup, and salt volume fraction, verifying the viability of reducing actinide waste production with this concept. Additional analyses of mass feed rates and enrichments, isotopic removals, tritium generation, core power distribution, core vessel helium generation, moderator rod heat deposition, and reactivity coeffcients provide additional information to make informed design decisions. This work demonstrates capabilities of ORNL modeling and simulation tools for neutronic and fuel cycle analysis of molten salt reactor concepts.« less

Post impact behavior of mobile reactor core containment systems

NASA Technical Reports Server (NTRS)

Puthoff, R. L.; Parker, W. G.; Vanbibber, L. E.

1972-01-01

The reactor core containment vessel temperatures after impact, and the design variables that affect the post impact survival of the system are analyzed. The heat transfer analysis includes conduction, radiation, and convection in addition to the core material heats of fusion and vaporization under partially burial conditions. Also, included is the fact that fission products vaporize and transport radially outward and condense outward and condense on cooler surfaces, resulting in a moving heat source. A computer program entitled Executive Subroutines for Afterheat Temperature Analysis (ESATA) was written to consider this complex heat transfer analysis. Seven cases were calculated of a reactor power system capable of delivering up to 300 MW of thermal power to a nuclear airplane.

Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cells.

PubMed

Li, LiQi; Jothi, Raja; Cui, Kairong; Lee, Jan Y; Cohen, Tsadok; Gorivodsky, Marat; Tzchori, Itai; Zhao, Yangu; Hayes, Sandra M; Bresnick, Emery H; Zhao, Keji; Westphal, Heiner; Love, Paul E

2011-02-01

The nuclear adaptor Ldb1 functions as a core component of multiprotein transcription complexes that regulate differentiation in diverse cell types. In the hematopoietic lineage, Ldb1 forms a complex with the non-DNA-binding adaptor Lmo2 and the transcription factors E2A, Scl and GATA-1 (or GATA-2). Here we demonstrate a critical and continuous requirement for Ldb1 in the maintenance of both fetal and adult mouse hematopoietic stem cells (HSCs). Deletion of Ldb1 in hematopoietic progenitors resulted in the downregulation of many transcripts required for HSC maintenance. Genome-wide profiling by chromatin immunoprecipitation followed by sequencing (ChIP-Seq) identified Ldb1 complex-binding sites at highly conserved regions in the promoters of genes involved in HSC maintenance. Our results identify a central role for Ldb1 in regulating the transcriptional program responsible for the maintenance of HSCs.

Identification of a Functional, CRM-1-Dependent Nuclear Export Signal in Hepatitis C Virus Core Protein

PubMed Central

Cerutti, Andrea; Maillard, Patrick; Minisini, Rosalba; Vidalain, Pierre-Olivier; Roohvand, Farzin; Pecheur, Eve-Isabelle; Pirisi, Mario; Budkowska, Agata

2011-01-01

Hepatitis C virus (HCV) infection is a major cause of chronic liver disease worldwide. HCV core protein is involved in nucleocapsid formation, but it also interacts with multiple cytoplasmic and nuclear molecules and plays a crucial role in the development of liver disease and hepatocarcinogenesis. The core protein is found mostly in the cytoplasm during HCV infection, but also in the nucleus in patients with hepatocarcinoma and in core-transgenic mice. HCV core contains nuclear localization signals (NLS), but no nuclear export signal (NES) has yet been identified. We show here that the aa(109–133) region directs the translocation of core from the nucleus to the cytoplasm by the CRM-1-mediated nuclear export pathway. Mutagenesis of the three hydrophobic residues (L119, I123 and L126) in the identified NES or in the sequence encoding the mature core aa(1–173) significantly enhanced the nuclear localisation of the corresponding proteins in transfected Huh7 cells. Both the NES and the adjacent hydrophobic sequence in domain II of core were required to maintain the core protein or its fragments in the cytoplasmic compartment. Electron microscopy studies of the JFH1 replication model demonstrated that core was translocated into the nucleus a few minutes after the virus entered the cell. The blockade of nucleocytoplasmic export by leptomycin B treatment early in infection led to the detection of core protein in the nucleus by confocal microscopy and coincided with a decrease in virus replication. Our data suggest that the functional NLS and NES direct HCV core protein shuttling between the cytoplasmic and nuclear compartments, with at least some core protein transported to the nucleus. These new properties of HCV core may be essential for virus multiplication and interaction with nuclear molecules, influence cell signaling and the pathogenesis of HCV infection. PMID:22039426

Structure and reconstitution of yeast Mpp6-nuclear exosome complexes reveals that Mpp6 stimulates RNA decay and recruits the Mtr4 helicase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wasmuth, Elizabeth V.; Zinder, John C.; Zattas, Dimitrios

Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase. Mpp6 interacts with the nine-subunit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 and recruits Mtr4, but it is less clear if these cofactors work together. Using biochemistry with Saccharomyces cerevisiae proteins, we show that Rrp47 and Mpp6 stimulate exosome-mediated RNA decay, albeit with unique dependencies on elements within the nuclear exosome. Mpp6-exosomes can recruit Mtr4, while Mpp6 and Rrp47 each contribute to Mtr4-dependent RNA decay, with maximal Mtr4-dependent decay observed with bothmore » cofactors. The 3.3 Å structure of a twelve-subunit nuclear Mpp6 exosome bound to RNA shows the central region of Mpp6 bound to the exosome core, positioning its Mtr4 recruitment domain next to Rrp6 and the exosome central channel. Genetic analysis reveals interactions that are largely consistent with our model.« less

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex.

PubMed

Obado, Samson O; Field, Mark C; Rout, Michael P

2017-07-04

The core architecture of the eukaryotic cell was established well over one billion years ago, and is largely retained in all extant lineages. However, eukaryotic cells also possess lineage-specific features, frequently keyed to specific functional requirements. One quintessential core eukaryotic structure is the nuclear pore complex (NPC), responsible for regulating exchange of macromolecules between the nucleus and cytoplasm as well as acting as a nuclear organizational hub. NPC architecture has been best documented in one eukaryotic supergroup, the Opisthokonts (e.g. Saccharomyces cerevisiae and Homo sapiens), which although compositionally similar, have significant variations in certain NPC subcomplex structures. The variation of NPC structure across other taxa in the eukaryotic kingdom however, remains poorly understood. We explored trypanosomes, highly divergent organisms, and mapped and assigned their NPC proteins to specific substructures to reveal their NPC architecture. We showed that the NPC central structural scaffold is conserved, likely across all eukaryotes, but more peripheral elements can exhibit very significant lineage-specific losses, duplications or other alterations in their components. Amazingly, trypanosomes lack the major components of the mRNA export platform that are asymmetrically localized within yeast and vertebrate NPCs. Concomitant with this, the trypanosome NPC is ALMOST completely symmetric with the nuclear basket being the only major source of asymmetry. We suggest these features point toward a stepwise evolution of the NPC in which a coating scaffold first stabilized the pore after which selective gating emerged and expanded, leading to the addition of peripheral remodeling machineries on the nucleoplasmic and cytoplasmic sides of the pore.

Fuel Cycle Performance of Thermal Spectrum Small Modular Reactors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Worrall, Andrew; Todosow, Michael

2016-01-01

Small modular reactors may offer potential benefits, such as enhanced operational flexibility. However, it is vital to understand the holistic impact of small modular reactors on the nuclear fuel cycle and fuel cycle performance. The focus of this paper is on the fuel cycle impacts of light water small modular reactors in a once-through fuel cycle with low-enriched uranium fuel. A key objective of this paper is to describe preliminary reactor core physics and fuel cycle analyses conducted in support of the U.S. Department of Energy Office of Nuclear Energy Fuel Cycle Options Campaign. Challenges with small modular reactors include:more » increased neutron leakage, fewer assemblies in the core (and therefore fewer degrees of freedom in the core design), complex enrichment and burnable absorber loadings, full power operation with inserted control rods, the potential for frequent load-following operation, and shortened core height. Each of these will impact the achievable discharge burn-up in the reactor and the fuel cycle performance. This paper summarizes the results of an expert elicitation focused on developing a list of the factors relevant to small modular reactor fuel, core, and operation that will impact fuel cycle performance. Preliminary scoping analyses were performed using a regulatory-grade reactor core simulator. The hypothetical light water small modular reactor considered in these preliminary scoping studies is a cartridge type one-batch core with 4.9% enrichment. Some core parameters, such as the size of the reactor and general assembly layout, are similar to an example small modular reactor concept from industry. The high-level issues identified and preliminary scoping calculations in this paper are intended to inform on potential fuel cycle impacts of one-batch thermal spectrum SMRs. In particular, this paper highlights the impact of increased neutron leakage and reduced number of batches on the achievable burn-up of the reactor. Fuel cycle performance metrics for a small modular reactor are compared to a conventional three-batch light water reactor in the following areas: nuclear waste management, environmental impact, and resource utilization. Metrics performance for a small modular reactor are degraded for mass of spent nuclear fuel and high level waste disposed, mass of depleted uranium disposed, land use per energy generated, and carbon emission per energy generated« less

Regulated transport into the nucleus of herpesviridae DNA replication core proteins.

PubMed

Gualtiero, Alvisi; Jans, David A; Camozzi, Daria; Avanzi, Simone; Loregian, Arianna; Ripalti, Alessandro; Palù, Giorgio

2013-09-16

The Herpesvirdae family comprises several major human pathogens belonging to three distinct subfamilies. Their double stranded DNA genome is replicated in the nuclei of infected cells by a number of host and viral products. Among the latter the viral replication complex, whose activity is strictly required for viral replication, is composed of six different polypeptides, including a two-subunit DNA polymerase holoenzyme, a trimeric primase/helicase complex and a single stranded DNA binding protein. The study of herpesviral DNA replication machinery is extremely important, both because it provides an excellent model to understand processes related to eukaryotic DNA replication and it has important implications for the development of highly needed antiviral agents. Even though all known herpesviruses utilize very similar mechanisms for amplification of their genomes, the nuclear import of the replication complex components appears to be a heterogeneous and highly regulated process to ensure the correct spatiotemporal localization of each protein. The nuclear transport process of these enzymes is controlled by three mechanisms, typifying the main processes through which protein nuclear import is generally regulated in eukaryotic cells. These include cargo post-translational modification-based recognition by the intracellular transporters, piggy-back events allowing coordinated nuclear import of multimeric holoenzymes, and chaperone-assisted nuclear import of specific subunits. In this review we summarize these mechanisms and discuss potential implications for the development of antiviral compounds aimed at inhibiting the Herpesvirus life cycle by targeting nuclear import of the Herpesvirus DNA replicating enzymes.

Core filaments of the nuclear matrix

PubMed Central

1990-01-01

The nuclear matrix is concealed by a much larger mass of chromatin, which can be removed selectively by digesting nuclei with DNase I followed by elution of chromatin with 0.25 M ammonium sulfate. This mild procedure removes chromatin almost completely and preserves nuclear matrix morphology. The complete nuclear matrix consists of a nuclear lamina with an interior matrix composed of thick, polymorphic fibers and large masses that resemble remnant nucleoli. Further extraction of the nuclear matrices of HeLa or MCF-7 cells with 2 M sodium chloride uncovered a network of core filaments. A few dark masses remained enmeshed in the filament network and may be remnants of the nuclear matrix thick fibers and nucleoli. The highly branched core filaments had diameters of 9 and 13 nm measured relative to the intermediate filaments. They may serve as the core structure around which the matrix is constructed. The core filaments retained 70% of nuclear RNA. This RNA consisted both of ribosomal RNA precursors and of very high molecular weight hnRNA with a modal size of 20 kb. Treatment with RNase A removed the core filaments. When 2 M sodium chloride was used directly to remove chromatin after DNase I digestion without a preceding 0.25 M ammonium sulfate extraction, the core filaments were not revealed. Instead, the nuclear interior was filled with amorphous masses that may cover the filaments. This reflected a requirement for a stepwise increase in ionic strength because gradual addition of sodium chloride to a final concentration of 2 M without an 0.25 M ammonium sulfate extraction uncovered core filaments. PMID:2307700

Developmental Loss of Photosystem II Activity and Structure in a Chloroplast-Encoded Tobacco Mutant, Lutescens-11

PubMed Central

Chia, Catherine P.; Duesing, John H.; Arntzen, Charles J.

1986-01-01

Lutescens-1, a tobacco mutant with a maternally inherited dysfunction, displayed an unusual developmental phenotype. In vivo measurement of chlorophyll fluorescence revealed deterioration in photosystem II (PSII) function as leaves expanded. Analysis of thylakoid membrane proteins by polyacrylamide gel electrophoresis indicated the physical loss of nuclear- and chloroplast-encoded polypeptides comprising the PSII core complex concomitant with loss of activity. Freeze fracture electron micrographs of mutant thylakoids showed a reduced density, compared to wild type, of the EFs particles which have been shown previously to be the structural entity containing PSII core complexes and associated pigment-proteins. The selective loss of PSII cores from thylakoids resulted in a higher ratio of antenna chlorophyll to reaction centers and an altered 77 K chlorophyll fluorescence emission spectra; these data are interpreted to indicate functional isolation of light-harvesting chlorophyll a/b complexes in the absence of PSII centers. Examination of PSII reaction centers (which were present at lower levels in mutant membranes) by monitoring the light-dependent phosphorylation of PSII polypeptides and flash-induced O2 evolution patterns demonstrated that the PSII cores which were assembled in mutant thylakoids were functionally identical to those of wild type. We conclude that the lutescens-1 mutation affected the correct stoichiometry of PSII centers, in relation to other membrane constituents, by disrupting the proper assembly and maintenance of PSII complexes in lutescens-1 thylakoid membranes. Images Fig. 4 Fig. 5 Fig. 6 Fig. 7 PMID:16664990

FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3.

PubMed

Wilson, J B; Yamamoto, K; Marriott, A S; Hussain, S; Sung, P; Hoatlin, M E; Mathew, C G; Takata, M; Thompson, L H; Kupfer, G M; Jones, N J

2008-06-12

Fanconi anemia (FA) is a human disorder characterized by cancer susceptibility and cellular sensitivity to DNA crosslinks and other damages. Thirteen complementation groups and genes are identified, including BRCA2, which is defective in the FA-D1 group. Eight of the FA proteins, including FANCG, participate in a nuclear core complex that is required for the monoubiquitylation of FANCD2 and FANCI. FANCD2, like FANCD1/BRCA2, is not part of the core complex, and we previously showed direct BRCA2-FANCD2 interaction using yeast two-hybrid analysis. We now show in human and hamster cells that expression of FANCG protein, but not the other core complex proteins, is required for co-precipitation of BRCA2 and FANCD2. We also show that phosphorylation of FANCG serine 7 is required for its co-precipitation with BRCA2, XRCC3 and FANCD2, as well as the direct interaction of BRCA2-FANCD2. These results argue that FANCG has a role independent of the FA core complex, and we propose that phosphorylation of serine 7 is the signalling event required for forming a discrete complex comprising FANCD1/BRCA2-FANCD2-FANCG-XRCC3 (D1-D2-G-X3). Cells that fail to express either phospho-Ser7-FANCG, or full length BRCA2 protein, lack the interactions amongst the four component proteins. A role for D1-D2-G-X3 in homologous recombination repair (HRR) is supported by our finding that FANCG and the RAD51-paralog XRCC3 are epistatic for sensitivity to DNA crosslinking compounds in DT40 chicken cells. Our findings further define the intricate interface between FANC and HRR proteins in maintaining chromosome stability.

Nuclear mRNA Surveillance Mechanisms: Function and Links to Human Disease.

PubMed

Singh, Pragyan; Saha, Upasana; Paira, Sunirmal; Das, Biswadip

2018-05-11

Production of export-competent mRNAs involves transcription and a series of dynamic processing and modification events of pre-messenger RNAs in the nucleus. Mutations in the genes encoding the transcription and mRNP processing machinery and the complexities involved in the biogenesis events lead to the formation of aberrant messages. These faulty transcripts are promptly eliminated by the nuclear RNA exosome and its cofactors to safeguard the cells and organisms from genetic catastrophe. Mutations in the components of the core nuclear exosome and its cofactors lead to the tissue-specific dysfunction of exosomal activities, which are linked to diverse human diseases and disorders. In this article, we examine the structure and function of both the yeast and human RNA exosome complex and its cofactors, discuss the nature of the various altered amino acid residues implicated in these diseases with the speculative mechanisms of the mutation-induced disorders and project the frontier and prospective avenues of the future research in this field. Copyright © 2018 Elsevier Ltd. All rights reserved.

Nuclear Involvement in the Appearance of a Chloroplast-Encoded 32,000 Dalton Thylakoid Membrane Polypeptide Integral to the Photosystem II Complex 1

PubMed Central

Leto, Kenneth J.; Keresztes, Aron; Arntzen, Charles J.

1982-01-01

The genetic locus for the high chlorophyll fluorescent photosystem II-deficient maize mutant hcf*-3 has been definitively located to the nuclear genome. Fluorography of lamellar polypeptides labeled with [35S]methionine in vivo revealed the specific loss of a heavily labeled 32,000 dalton thylakoid membrane polypeptide as well as its chloroplast encoded precursor species at 34,000 daltons. Examination of freeze-fractured mesophyll and bundle sheath thylakoids from hcf*-3 revealed that both plastid types lacked the large EFs particles believed to consist of the photosystem II reaction center-core complex and associated light harvesting chlorophyll-proteins. The present evidence suggests that the synthesis or turnover/integration of the chloroplast-encoded 34,000 to 32,000 dalton polypeptide is under nuclear control, and that these polyipeptides are integral components of photosystem II which may be required for the assembly or structural stabilization of newly formed photosystem II reaction centers in both mesophyll and bundle sheath chloroplasts. Images PMID:16662421

Autonomous Control of Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Basher, H.

2003-10-20

A nuclear reactor is a complex system that requires highly sophisticated controllers to ensure that desired performance and safety can be achieved and maintained during its operations. Higher-demanding operational requirements such as reliability, lower environmental impacts, and improved performance under adverse conditions in nuclear power plants, coupled with the complexity and uncertainty of the models, necessitate the use of an increased level of autonomy in the control methods. In the opinion of many researchers, the tasks involved during nuclear reactor design and operation (e.g., design optimization, transient diagnosis, and core reload optimization) involve important human cognition and decisions that maymore » be more easily achieved with intelligent methods such as expert systems, fuzzy logic, neural networks, and genetic algorithms. Many experts in the field of control systems share the idea that a higher degree of autonomy in control of complex systems such as nuclear plants is more easily achievable through the integration of conventional control systems and the intelligent components. Researchers have investigated the feasibility of the integration of fuzzy logic, neural networks, genetic algorithms, and expert systems with the conventional control methods to achieve higher degrees of autonomy in different aspects of reactor operations such as reactor startup, shutdown in emergency situations, fault detection and diagnosis, nuclear reactor alarm processing and diagnosis, and reactor load-following operations, to name a few. With the advancement of new technologies and computing power, it is feasible to automate most of the nuclear reactor control and operation, which will result in increased safety and economical benefits. This study surveys current status, practices, and recent advances made towards developing autonomous control systems for nuclear reactors.« less

Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the cellular NXF1-p15 machinery.

PubMed

Yang, Ching-Chun; Huang, Er-Yi; Li, Hung-Cheng; Su, Pei-Yi; Shih, Chiaho

2014-01-01

Hepatitis B virus (HBV) core protein (HBc) can shuttle between nucleus and cytoplasm. Cytoplasm-predominant HBc is clinically associated with severe liver inflammation. Previously, we found that HBc arginine-rich domain (ARD) can associate with a host factor NXF1 (TAP) by coimmunoprecipitation. It is well known that NXF1-p15 heterodimer can serve as a major export receptor of nuclear mRNA as a ribonucleoprotein complex (RNP). In the NXF1-p15 pathway, TREX (transcription/export) complex plays an important role in coupling nuclear pre-mRNA processing with mRNA export in mammalian cells. Here, we tested the hypothesis whether HBc and HBV specific RNA can be exported via the TREX and NXF1-p15 mediated pathway. We demonstrated here that HBc can physically and specifically associate with TREX components, and the NXF1-p15 export receptor by coimmunoprecipitation. Accumulation of HBc protein in the nucleus can be induced by the interference with TREX and NXF1-p15 mediated RNA export machinery. HBV transcripts encodes a non-spliced 3.5 kb pregenomic RNA (pgRNA) which can serve as a template for reverse transcription. Cytoplasmic HBV pgRNA appeared to be reduced by siRNA treatment specific for the NXF1-p15 complex by quantitative RT-qPCR and Northern blot analyses. This result suggests that the pgRNA was also exported via the NXF1-p15 machinery. We entertain the hypothesis that HBc protein can be exported as an RNP cargo via the mRNA export pathway by hijacking the TREX and NXF1-p15 complex. In our current and previous studies, HBc is not required for pgRNA accumulation in the cytoplasm. Furthermore, HBc ARD can mediate nuclear export of a chimeric protein containing HBc ARD in a pgRNA-independent manner. Taken together, it suggests that while both pgRNA and HBc protein exports are dependent on NXF1-p15, they are using the same export machinery in a manner independent of each other.

Design requirements for innovative homogeneous reactor, lesson learned from Fukushima accident

NASA Astrophysics Data System (ADS)

Arbie, Bakri; Pinem, Suryan; Sembiring, Tagor; Subki, Iyos

2012-06-01

The Fukushima disaster is the largest nuclear accident since the 1986 Chernobyl disaster, but it is more complex as multiple reactors and spent fuel pools are involved. The severity of the nuclear accident is rated 7 in the International Nuclear Events Scale. Expert said that "Fukushima is the biggest industrial catastrophe in the history of mankind". According to Mitsuru Obe, in The Wall Street Journal, May 16th of 2011, TEPCO estimates the nuclear fuel was exposed to the air less than five hours after the earthquake struck. Fuel rods melted away rapidly as the temperatures inside the core reached 2800 C within six hours. In less than 16 hours, the reactor core melted and dropped to the bottom of the pressure vessel. The information should be evaluated in detail. In Germany several nuclear power plant were shutdown, Italy postponed it's nuclear power program and China reviewed their nuclear power program. Different news come from Britain, in October 11, 2011, the Safety Committee said all clear for nuclear power in Britain, because there are no risk of strong earthquake and tsunami in the region. Due to this severe fact, many nuclear scientists and engineer from all over the world are looking for a new approach, such as homogeneous reactor which was developed in Oak Ridge National Laboratory in 1960-ies, during Dr. Alvin Weinberg tenure as the Director of ORNL. The paper will describe the design requirement that will be used as the basis for innovative homogeneous reactor. Innovative Homogeneous Reactor is expected to reduce core melt by two decades (4), since the fuel is intermix homogeneously with coolant and secondly we eliminate the used fuel rod which need to be cooled for a long period of time. In order to be successful for its implementation of the innovative system, testing and validation, three phases of development will be introduced. The first phase is Low Level Goals is really the proof of concept;the Medium Level Goal is Technical Goalsand the High Level Goals which is Business Goals.

Bcs1p can rescue a large and productive cytochrome bc(1) complex assembly intermediate in the inner membrane of yeast mitochondria.

PubMed

Conte, Laura; Trumpower, Bernard L; Zara, Vincenzo

2011-01-01

The yeast cytochrome bc(1) complex, a component of the mitochondrial respiratory chain, is composed of ten distinct protein subunits. In the assembly of the bc(1) complex, some ancillary proteins, such as the chaperone Bcs1p, are actively involved. The deletion of the nuclear gene encoding this chaperone caused the arrest of the bc(1) assembly and the formation of a functionally inactive bc(1) core structure of about 500-kDa. This immature bc(1) core structure could represent, on the one hand, a true assembly intermediate or, on the other hand, a degradation product and/or an incorrect product of assembly. The experiments here reported show that the gradual expression of Bcs1p in the yeast strain lacking this protein was progressively able to rescue the bc(1) core structure leading to the formation of the functional homodimeric bc(1) complex. Following Bcs1p expression, the mature bc(1) complex was also progressively converted into two supercomplexes with the cytochrome c oxidase complex. The capability of restoring the bc(1) complex and the supercomplexes was also possessed by the mutated yeast R81C Bcsp1. Notably, in the human ortholog BCS1L, the corresponding point mutation (R45C) was instead the cause of a severe bc(1) complex deficiency. Differently from the yeast R81C Bcs1p, two other mutated Bcs1p's (K192P and F401I) were unable to recover the bc(1) core structure in yeast. This study identifies for the first time a productive assembly intermediate of the yeast bc(1) complex and gives new insights into the molecular mechanisms involved in the last steps of bc(1) assembly. Copyright © 2010 Elsevier B.V. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, Nicholas R.; Worrall, Andrew; Todosow, Michael

Small modular reactors (SMRs) offer potential benefits, such as enhanced operational flexibility. However, it is vital to understand the holistic impact of SMRs on nuclear fuel cycle performance. The focus of this paper is the fuel cycle impacts of light water SMRs in a once-through fuel cycle with low-enriched uranium fuel. A key objective of this paper is to describe preliminary example reactor core physics and fuel cycle analyses conducted in support of the U.S. Department of Energy, Office of Nuclear Energy, Fuel Cycle Options Campaign. The hypothetical light water SMR example case considered in these preliminary scoping studies ismore » a cartridge type one-batch core with slightly less than 5.0% enrichment. Challenges associated with SMRs include increased neutron leakage, fewer assemblies in the core (and therefore fewer degrees of freedom in the core design), complex enrichment and burnable absorber loadings, full power operation with inserted control rods, the potential for frequent load-following operation, and shortened core height. Each of these will impact the achievable discharge burnup in the reactor and the fuel cycle performance. This paper summarizes a list of the factors relevant to SMR fuel, core, and operation that will impact fuel cycle performance. The high-level issues identified and preliminary scoping calculations in this paper are intended to inform on potential fuel cycle impacts of one-batch thermal spectrum SMRs. In particular, this paper highlights the impact of increased neutron leakage and reduced number of batches on the achievable burnup of the reactor. Fuel cycle performance metrics for a hypothetical example SMR are compared with those for a conventional three-batch light water reactor in the following areas: nuclear waste management, environmental impact, and resource utilization. The metrics performance for such an SMR is degraded for the mass of spent nuclear fuel and high-level waste disposed of, mass of depleted uranium disposed of, land use per energy generated, and carbon emissions per energy generated. Finally, it is noted that the features of some SMR designs impact three main aspects of fuel cycle performance: (1) small cores which means high leakage (there is a radial and axial component), (2) no boron which means heterogeneous core and extensive use of control rods and BPs, and (3) single batch cores. But not all of the SMR designs have all of these traits. As a result, the approach used in this study is therefore a bounding case and not all SMRs may be affected to the same extent.« less

Two-Dimensional Neutronic and Fuel Cycle Analysis of the Transatomic Power Molten Salt Reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Betzler, Benjamin R.; Powers, Jeffrey J.; Worrall, Andrew

2017-01-15

This status report presents the results from the first phase of the collaboration between Transatomic Power Corporation (TAP) and Oak Ridge National Laboratory (ORNL) to provide neutronic and fuel cycle analysis of the TAP core design through the Department of Energy Gateway for Accelerated Innovation in Nuclear, Nuclear Energy Voucher program. The TAP design is a molten salt reactor using movable moderator rods to shift the neutron spectrum in the core from mostly epithermal at beginning of life to thermal at end of life. Additional developments in the ChemTriton modeling and simulation tool provide the critical moderator-to-fuel ratio searches andmore » time-dependent parameters necessary to simulate the continuously changing physics in this complex system. Results from simulations with these tools show agreement with TAP-calculated performance metrics for core lifetime, discharge burnup, and salt volume fraction, verifying the viability of reducing actinide waste production with this design. Additional analyses of time step sizes, mass feed rates and enrichments, and isotopic removals provide additional information to make informed design decisions. This work further demonstrates capabilities of ORNL modeling and simulation tools for analysis of molten salt reactor designs and strongly positions this effort for the upcoming three-dimensional core analysis.« less

Nuclear envelopathies: a complex LINC between nuclear envelope and pathology.

PubMed

Janin, Alexandre; Bauer, Delphine; Ratti, Francesca; Millat, Gilles; Méjat, Alexandre

2017-08-30

Since the identification of the first disease causing mutation in the gene coding for emerin, a transmembrane protein of the inner nuclear membrane, hundreds of mutations and variants have been found in genes encoding for nuclear envelope components. These proteins can be part of the inner nuclear membrane (INM), such as emerin or SUN proteins, outer nuclear membrane (ONM), such as Nesprins, or the nuclear lamina, such as lamins A and C. However, they physically interact with each other to insure the nuclear envelope integrity and mediate the interactions of the nuclear envelope with both the genome, on the inner side, and the cytoskeleton, on the outer side. The core of this complex, called LINC (LInker of Nucleoskeleton to Cytoskeleton) is composed of KASH and SUN homology domain proteins. SUN proteins are INM proteins which interact with lamins by their N-terminal domain and with the KASH domain of nesprins located in the ONM by their C-terminal domain.Although most of these proteins are ubiquitously expressed, their mutations have been associated with a large number of clinically unrelated pathologies affecting specific tissues. Moreover, variants in SUN proteins have been found to modulate the severity of diseases induced by mutations in other LINC components or interactors. For these reasons, the diagnosis and the identification of the molecular explanation of "nuclear envelopathies" is currently challenging.The aim of this review is to summarize the human diseases caused by mutations in genes coding for INM proteins, nuclear lamina, and ONM proteins, and to discuss their potential physiopathological mechanisms that could explain the large spectrum of observed symptoms.

Supernovae neutrino pasta interaction

NASA Astrophysics Data System (ADS)

Lin, Zidu; Horowitz, Charles; Caplan, Matthew; Berry, Donald; Roberts, Luke

2017-01-01

In core-collapse supernovae, the neutron rich matter is believed to have complex structures, such as spherical, slablike, and rodlike shapes. They are collectively called ``nuclear pasta''. Supernovae neutrinos may scatter coherently on the ``nuclear pasta'' since the wavelength of the supernovae neutrinos are comparable to the nuclear pasta scale. Consequently, the neutrino pasta scattering is important to understand the neutrino opacity in the supernovae. In this work we simulated the ``nuclear pasta'' at different temperatures and densities using our semi-classical molecular dynamics and calculated the corresponding static structure factor that describes ν-pasta scattering. We found the neutrino opacities are greatly modified when the ``pasta'' exist and may have influence on the supernovae neutrino flux and average energy. Our neutrino-pasta scattering effect can finally be involved in the current supernovae simulations and we present preliminary proto neutron star cooling simulations including our pasta opacities.

Facile CO Cleavage by a Multimetallic CsU2 Nitride Complex.

PubMed

Falcone, Marta; Kefalidis, Christos E; Scopelliti, Rosario; Maron, Laurent; Mazzanti, Marinella

2016-09-26

Uranium nitrides are important materials with potential for application as fuels for nuclear power generation, and as highly active catalysts. Molecular nitride compounds could provide important insight into the nature of the uranium-nitride bond, but currently little is known about their reactivity. In this study, we found that a complex containing a nitride bridging two uranium centers and a cesium cation readily cleaved the C≡O bond (one of the strongest bonds in nature) under ambient conditions. The product formed has a [CsU2 (μ-CN)(μ-O)] core, thus indicating that the three cations cooperate to cleave CO. Moreover, the addition of MeOTf to the nitride complex led to an exceptional valence disproportionation of the CsU(IV) -N-U(IV) core to yield CsU(III) (OTf) and [MeN=U(V) ] fragments. The important role of multimetallic cooperativity in both reactions is illustrated by the computed reaction mechanisms. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comments on the feasibility of developing gas core nuclear reactors. [for manned interplanetary spacecraft propulsion

NASA Technical Reports Server (NTRS)

Rom, F. E.

1969-01-01

Recent developments in the fields of gas core hydrodynamics, heat transfer, and neutronics indicate that gas core nuclear rockets may be feasible from the point of view of basic principles. Based on performance predictions using these results, mission analyses indicate that gas core nuclear rockets may have the potential for reducing the initial weight in orbit of manned interplanetary vehicles by a factor of 5 when compared to the best chemical rocket systems. In addition, there is a potential for reducing total trip times from 450 to 500 days for chemical systems to 250 to 300 days for gas core systems. The possibility of demonstrating the feasibility of gas core nuclear rocket engines by means of a logical series of experiments of increasing difficulty that ends with ground tests of full scale gas core reactors is considered.

BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks

PubMed Central

Pichierri, Pietro; Franchitto, Annapaola; Rosselli, Filippo

2004-01-01

Fanconi anaemia (FA) and Bloom syndrome (BS) are autosomal recessive diseases characterised by chromosome fragility and cancer proneness. Here, we report that BLM and the FA pathway are activated in response to both crosslinked DNA and replication fork stall. We provide evidence that BLM and FANCD2 colocalise and co-immunoprecipitate following treatment with either DNA crosslinkers or agents inducing replication arrest. We also find that the FA core complex is necessary for BLM phosphorylation and assembly in nuclear foci in response to crosslinked DNA. Moreover, we show that knock-down of the MRE11 complex, whose function is also under the control of the FA core complex, enhances cellular and chromosomal sensitivity to DNA interstrand crosslinks in BS cells. These findings suggest the existence of a functional link between BLM and the FA pathway and that BLM and the MRE11 complex are in two separated branches of a pathway resulting in S-phase checkpoint activation, chromosome integrity and cell survival in response to crosslinked DNA. PMID:15257300

Kilopower: Small and Affordable Fission Power Systems for Space

NASA Technical Reports Server (NTRS)

Mason, Lee; Palac, Don; Gibson, Marc

2017-01-01

The Nuclear Systems Kilopower Project was initiated by NASA's Space Technology Mission Directorate Game Changing Development Program in fiscal year 2015 to demonstrate subsystem-level technology readiness of small space fission power in a relevant environment (Technology Readiness Level 5) for space science and human exploration power needs. The Nuclear Systems Kilopower Project centerpiece is the Kilopower Reactor Using Stirling Technology (KRUSTY) test, which consists of the development and testing of a fission ground technology demonstrator of a 1 kWe-class fission power system. The technologies to be developed and validated by KRUSTY are extensible to space fission power systems from 1 to 10 kWe, which can enable higher power future potential deep space science missions, as well as modular surface fission power systems for exploration. The Kilopower Project is cofounded by NASA and the Department of Energy National Nuclear Security Administration (NNSA).KRUSTY include the reactor core, heat pipes to transfer the heat from the core to the power conversion system, and the power conversion system. Los Alamos National Laboratory leads the design of the reactor, and the Y-12 National Security Complex is fabricating it. NASA Glenn Research Center (GRC) has designed, built, and demonstrated the balance of plant heat transfer and power conversion portions of the KRUSTY experiment. NASA MSFC developed an electrical reactor simulator for non-nuclear testing, and the design of the reflector and shielding for nuclear testing. In 2016, an electrically heated non-fissionable Depleted Uranium (DU) core was tested at GRC in a configuration identical to the planned nuclear test. Once the reactor core has been fabricated and shipped to the Device Assembly Facility at the NNSAs Nevada National Security Site, the KRUSTY nuclear experiment will be assembled and tested. Completion of the KRUSTY experiment will validate the readiness of 1 to 10 kWe space fission technology for NASAs future requirements for sunlight-independent space power. An early opportunity for demonstration of In-Situ Resource Utilization (ISRU) capability on the surface of Mars is currently being considered for 2026 launch. Since a space fission system is the leading option for power generation for the first Mars human outpost, a smaller version of a planetary surface fission power system could be built to power the ISRU demonstration and ensure its end-to-end validity. Planning is underway to start the hardware development of this subscale flight demonstrator in 2018.

Performance potential of gas-core and fusion rockets - A mission applications survey.

NASA Technical Reports Server (NTRS)

Fishbach, L. H.; Willis, E. A., Jr.

1971-01-01

This paper reports an evaluation of the performance potential of five nuclear rocket engines for four mission classes. These engines are: the regeneratively cooled gas-core nuclear rocket; the light bulb gas-core nuclear rocket; the space-radiator cooled gas-core nuclear rocket; the fusion rocket; and an advanced solid-core nuclear rocket which is included for comparison. The missions considered are: earth-to-orbit launch; near-earth space missions; close interplanetary missions; and distant interplanetary missions. For each of these missions, the capabilities of each rocket engine type are compared in terms of payload ratio for the earth launch mission or by the initial vehicle mass in earth orbit for space missions (a measure of initial cost). Other factors which might determine the engine choice are discussed. It is shown that a 60 day manned round trip to Mars is conceivable.-

Cytoplasmic FANCA-FANCC Complex Interacts and Stabilizes the Cytoplasm-dislocalized Leukemic Nucleophosmin Protein (NPMc)*

PubMed Central

Du, Wei; Li, Jie; Sipple, Jared; Chen, Jianjun; Pang, Qishen

2010-01-01

Eight of the Fanconi anemia (FA) proteins form a core complex that activates the FA pathway. Some core complex components also form subcomplexes for yet-to-be-elucidated functions. Here, we have analyzed the interaction between a cytoplasmic FA subcomplex and the leukemic nucleophosmin (NPMc). Exogenous NPMc was degraded rapidly in FA acute myeloid leukemia bone marrow cells. Knockdown of FANCA or FANCC in leukemic OCI/AML3 cells induced rapid degradation of endogenous NPMc. NPMc degradation was mediated by the ubiquitin-proteasome pathway involving the IBR-type RING-finger E3 ubiquitin ligase IBRDC2, and genetic correction of FA-A or FA-C lymphoblasts prevented NPMc ubiquitination. Moreover, cytoplasmic FANCA and FANCC formed a cytoplasmic complex and interacted with NPMc. Using a patient-derived FANCC mutant and a nuclearized FANCC, we demonstrated that the cytoplasmic FANCA-FANCC complex was essential for NPMc stability. Finally, depletion of FANCA and FANCC in NPMc-positive leukemic cells significantly increased inflammation and chemoresistance through NF-κB activation. Our findings not only reveal the molecular mechanism involving cytoplasmic retention of NPMc but also suggest cytoplasmic function of FANCA and FANCC in NPMc-related leukemogenesis. PMID:20864535

Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex.

PubMed

Weick, Eva-Maria; Puno, M Rhyan; Januszyk, Kurt; Zinder, John C; DiMattia, Michael A; Lima, Christopher D

2018-06-14

The ribonucleolytic RNA exosome interacts with RNA helicases to degrade RNA. To understand how the 3' to 5' Mtr4 helicase engages RNA and the nuclear exosome, we reconstituted 14-subunit Mtr4-containing RNA exosomes from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and human and show that they unwind structured substrates to promote degradation. We loaded a human exosome with an optimized DNA-RNA chimera that stalls MTR4 during unwinding and determined its structure to an overall resolution of 3.45 Å by cryoelectron microscopy (cryo-EM). The structure reveals an RNA-engaged helicase atop the non-catalytic core, with RNA captured within the central channel and DIS3 exoribonuclease active site. MPP6 tethers MTR4 to the exosome through contacts to the RecA domains of MTR4. EXOSC10 remains bound to the core, but its catalytic module and cofactor C1D are displaced by RNA-engaged MTR4. Competition for the exosome core may ensure that RNA is committed to degradation by DIS3 when engaged by MTR4. Copyright © 2018 Elsevier Inc. All rights reserved.

Some features of the effect the pH value and the physicochemical properties of boric acid have on mass transfer in a VVER reactor's core

NASA Astrophysics Data System (ADS)

Gavrilov, A. V.; Kritskii, V. G.; Rodionov, Yu. A.; Berezina, I. G.

2013-07-01

Certain features of the effect of boric acid in the reactor coolant of nuclear power installations equipped with a VVER-440 reactor on mass transfer in the reactor core are considered. It is determined that formation of boric acid polyborate complexes begins under field conditions at a temperature of 300°C when the boric acid concentration is equal to around 0.065 mol/L (4 g/L). Operations for decontaminating the reactor coolant system entail a growth of corrosion product concentration in the coolant, which gives rise to formation of iron borates in the zones where subcooled boiling of coolant takes place and to the effect of axial offset anomalies. A model for simulating variation of pressure drop in a VVER-440 reactor's core that has invariable parameters during the entire fuel campaign is developed by additionally taking into account the concentrations of boric acid polyborate complexes and the quantity of corrosion products (Fe, Ni) represented by the ratio of their solubilities.

Turbulence coefficients and stability studies for the coaxial flow or dissimiliar fluids. [gaseous core nuclear reactors

NASA Technical Reports Server (NTRS)

Weinstein, H.; Lavan, Z.

1975-01-01

Analytical investigations of fluid dynamics problems of relevance to the gaseous core nuclear reactor program are presented. The vortex type flow which appears in the nuclear light bulb concept is analyzed along with the fluid flow in the fuel inlet region for the coaxial flow gaseous core nuclear reactor concept. The development of numerical methods for the solution of the Navier-Stokes equations for appropriate geometries is extended to the case of rotating flows and almost completes the gas core program requirements in this area. The investigations demonstrate that the conceptual design of the coaxial flow reactor needs further development.

Ultrahigh temperature vapor core reactor-MHD system for space nuclear electric power

NASA Technical Reports Server (NTRS)

Maya, Isaac; Anghaie, Samim; Diaz, Nils J.; Dugan, Edward T.

1991-01-01

The conceptual design of a nuclear space power system based on the ultrahigh temperature vapor core reactor with MHD energy conversion is presented. This UF4 fueled gas core cavity reactor operates at 4000 K maximum core temperature and 40 atm. Materials experiments, conducted with UF4 up to 2200 K, demonstrate acceptable compatibility with tungsten-molybdenum-, and carbon-based materials. The supporting nuclear, heat transfer, fluid flow and MHD analysis, and fissioning plasma physics experiments are also discussed.

Tetratricopeptide-motif-mediated interaction of FANCG with recombination proteins XRCC3 and BRCA2.

PubMed

Hussain, Shobbir; Wilson, James B; Blom, Eric; Thompson, Larry H; Sung, Patrick; Gordon, Susan M; Kupfer, Gary M; Joenje, Hans; Mathew, Christopher G; Jones, Nigel J

2006-05-10

Fanconi anaemia is an inherited chromosomal instability disorder characterised by cellular sensitivity to DNA interstrand crosslinkers, bone-marrow failure and a high risk of cancer. Eleven FA genes have been identified, one of which, FANCD1, is the breast cancer susceptibility gene BRCA2. At least eight FA proteins form a nuclear core complex required for monoubiquitination of FANCD2. The BRCA2/FANCD1 protein is connected to the FA pathway by interactions with the FANCG and FANCD2 proteins, both of which co-localise with the RAD51 recombinase, which is regulated by BRCA2. These connections raise the question of whether any of the FANC proteins of the core complex might also participate in other complexes involved in homologous recombination repair. We therefore tested known FA proteins for direct interaction with RAD51 and its paralogs XRCC2 and XRCC3. FANCG was found to interact with XRCC3, and this interaction was disrupted by the FA-G patient derived mutation L71P. FANCG was co-immunoprecipitated with both XRCC3 and BRCA2 from extracts of human and hamster cells. The FANCG-XRCC3 and FANCG-BRCA2 interactions did not require the presence of other FA proteins from the core complex, suggesting that FANCG also participates in a DNA repair complex that is downstream and independent of FANCD2 monoubiquitination. Additionally, XRCC3 and BRCA2 proteins co-precipitate in both human and hamster cells and this interaction requires FANCG. The FANCG protein contains multiple tetratricopeptide repeat motifs (TPRs), which function as scaffolds to mediate protein-protein interactions. Mutation of one or more of these motifs disrupted all of the known interactions of FANCG. We propose that FANCG, in addition to stabilising the FA core complex, may have a role in building multiprotein complexes that facilitate homologous recombination repair.

Magnetic nuclear core restraint and control

DOEpatents

Cooper, Martin H.

1979-01-01

A lateral restraint and control system for a nuclear reactor core adaptable to provide an inherent decrease of core reactivity in response to abnormally high reactor coolant fluid temperatures. An electromagnet is associated with structure for radially compressing the core during normal reactor conditions. A portion of the structures forming a magnetic circuit are composed of ferromagnetic material having a curie temperature corresponding to a selected coolant fluid temperature. Upon a selected signal, or inherently upon a preselected rise in coolant temperature, the magnetic force is decreased a given amount sufficient to relieve the compression force so as to allow core radial expansion. The expanded core configuration provides a decreased reactivity, tending to shut down the nuclear reaction.

Magnetic nuclear core restraint and control

DOEpatents

Cooper, Martin H.

1978-01-01

A lateral restraint and control system for a nuclear reactor core adaptable to provide an inherent decrease of core reactivity in response to abnormally high reactor coolant fluid temperatures. An electromagnet is associated with structure for radially compressing the core during normal reactor conditions. A portion of the structures forming a magnetic circuit are composed of ferromagnetic material having a curie temperature corresponding to a selected coolant fluid temperature. Upon a selected signal, or inherently upon a preselected rise in coolant temperature, the magnetic force is decreased a given amount sufficient to relieve the compression force so as to allow core radial expansion. The expanded core configuration provides a decreased reactivity, tending to shut down the nuclear reaction.

METHOD AND APPARATUS FOR EARTH PENETRATION

DOEpatents

Adams, W.M.

1963-12-24

A nuclear reactor apparatus for penetrating into the earth's crust is described. The apparatus comprises a cylindrical nuclear core operating at a temperature that is higher than the melting temperature of rock. A high-density ballast member is coupled to the nuclear core such that the overall density of the core-ballast assembly is greater than the density of molten rock. The nuclear core is thermally insulated so that its heat output is constrained to flow axially, with radial heat flow being minimized. In operation, the apparatus is placed in contact with the earth's crust at the point desired to be penetrated. The heat output of the reactor melts the underlying rock, and the apparatus sinks through the resulting magma. The fuel loading of the reactor core determines the ultimate depth of crust penetration. (AEC)

Adenovirus core protein VII contains distinct sequences that mediate targeting to the nucleus and nucleolus, and colocalization with human chromosomes.

PubMed

Lee, Tim W R; Blair, G Eric; Matthews, David A

2003-12-01

During adenovirus infection, following capsid dissociation, core protein VII enters the host cell nucleus complexed with adenovirus DNA. In order to determine whether protein VII may have an active role in this nuclear import, regions of the preVII gene were amplified by PCR, and further oligonucleotide mutants were designed with site-directed mutation of codons for the basic amino acids arginine and lysine. Fragments were cloned into a mammalian expression plasmid to express the peptides as N-terminal fusions to enhanced green fluorescent protein. Results demonstrate that preVII protein contains both nuclear and nucleolar targeting sequences. Such signals may be important in the delivery of adenovirus DNA to the host cell nucleus during adenovirus infection. Furthermore, the data suggest that protein VII may bind to human chromosomes by means of two distinct domains, one sharing homology with the N-terminal regulatory tail of histone H3.

Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cells

PubMed Central

Li, LiQi; Jothi, Raja; Cui, Kairong; Lee, Jan Y; Cohen, Tsadok; Gorivodsky, Marat; Tzchori, Itai; Zhao, Yangu; Hayes, Sandra M; Bresnick, Emery H; Zhao, Keji; Westphal, Heiner; Love, Paul E

2013-01-01

The nuclear adaptor Ldb1 functions as a core component of multiprotein transcription complexes that regulate differentiation in diverse cell types. In the hematopoietic lineage, Ldb1 forms a complex with the non–DNA-binding adaptor Lmo2 and the transcription factors E2A, Scl and GATA-1 (or GATA-2). Here we demonstrate a critical and continuous requirement for Ldb1 in the maintenance of both fetal and adult mouse hematopoietic stem cells (HSCs). Deletion of Ldb1 in hematopoietic progenitors resulted in the downregulation of many transcripts required for HSC maintenance. Genome-wide profiling by chromatin immunoprecipitation followed by sequencing (ChIP-Seq) identified Ldb1 complex–binding sites at highly conserved regions in the promoters of genes involved in HSC maintenance. Our results identify a central role for Ldb1 in regulating the transcriptional program responsible for the maintenance of HSCs. PMID:21186366

Proceedings of the 7th International Meeting on Nuclear Reactor Thermal-Hydraulics NURETH-7. Sessions 17-24

DOE Office of Scientific and Technical Information (OSTI.GOV)

Block, R.C.; Feiner, F.

1995-09-01

Technical papers accepted for presentation at the Seventh International Topical Meeting on Nuclear Reactor Thermal-Hydraulics are included in the present Proceedings. Except for the invited papers in the plenary session, all other papers are contributed papers. The topics of the meeting encompass all major areas of nuclear thermal-hydraulics, including analytical and experimental works on the fundamental mechanisms of fluid flow and heat transfer, the development of advanced mathematical and numerical methods, and the application of advancements in the field in the development of novel reactor concepts. Because of the complex nature of nuclear reactors and power plants, several papers dealmore » with the combined issues of thermal-hydraulics and reactor/power-plant safety, core neutronics and/or radiation. The participation in the conference by the authors from several countries and four continents makes the Proceedings a comprehensive review of the recent progress in the field of nuclear reactor thermal-hydraulics worldwide. Individual papers have been cataloged separately.« less

Modeling and simulation challenges pursued by the Consortium for Advanced Simulation of Light Water Reactors (CASL)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Turinsky, Paul J., E-mail: turinsky@ncsu.edu; Kothe, Douglas B., E-mail: kothe@ornl.gov

The Consortium for the Advanced Simulation of Light Water Reactors (CASL), the first Energy Innovation Hub of the Department of Energy, was established in 2010 with the goal of providing modeling and simulation (M&S) capabilities that support and accelerate the improvement of nuclear energy's economic competitiveness and the reduction of spent nuclear fuel volume per unit energy, and all while assuring nuclear safety. To accomplish this requires advances in M&S capabilities in radiation transport, thermal-hydraulics, fuel performance and corrosion chemistry. To focus CASL's R&D, industry challenge problems have been defined, which equate with long standing issues of the nuclear powermore » industry that M&S can assist in addressing. To date CASL has developed a multi-physics “core simulator” based upon pin-resolved radiation transport and subchannel (within fuel assembly) thermal-hydraulics, capitalizing on the capabilities of high performance computing. CASL's fuel performance M&S capability can also be optionally integrated into the core simulator, yielding a coupled multi-physics capability with untapped predictive potential. Material models have been developed to enhance predictive capabilities of fuel clad creep and growth, along with deeper understanding of zirconium alloy clad oxidation and hydrogen pickup. Understanding of corrosion chemistry (e.g., CRUD formation) has evolved at all scales: micro, meso and macro. CFD R&D has focused on improvement in closure models for subcooled boiling and bubbly flow, and the formulation of robust numerical solution algorithms. For multiphysics integration, several iterative acceleration methods have been assessed, illuminating areas where further research is needed. Finally, uncertainty quantification and data assimilation techniques, based upon sampling approaches, have been made more feasible for practicing nuclear engineers via R&D on dimensional reduction and biased sampling. Industry adoption of CASL's evolving M&S capabilities, which is in progress, will assist in addressing long-standing and future operational and safety challenges of the nuclear industry. - Highlights: • Complexity of physics based modeling of light water reactor cores being addressed. • Capability developed to help address problems that have challenged the nuclear power industry. • Simulation capabilities that take advantage of high performance computing developed.« less

Nuclear Neutrino Spectra in Late Stellar Evolution

NASA Astrophysics Data System (ADS)

Misch, G. Wendell; Sun, Yang; Fuller, George

2018-05-01

Neutrinos are the principle carriers of energy in massive stars, beginning from core carbon burning and continuing through core collapse and after the core bounce. In fact, it may be possible to detect neutrinos from nearby pre-supernova stars. Therefore, it is of great interest to understand the neutrino energy spectra from these stars. Leading up to core collapse, beginning around core silicon burning, nuclei become dominant producers of neutrinos, particularly at high neutrino energy, so a systematic study of nuclear neutrino spectra is desirable. We have done such a study, and we present our sd-shell model calculations of nuclear neutrino energy spectra for nuclei in the mass number range A = 21 - 35. Our study includes neutrinos produced by charged lepton capture, charged lepton emission, and neutral current nuclear deexcitation. Previous authors have tabulated the rates of charged current nuclear weak interactions in astrophysical conditions, but the present work expands on this not only by providing neutrino energy spectra, but also by including the heretofore untabulated neutral current de-excitation neutrino pairs.

Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing

PubMed Central

Kwak, Hojoong; Fuda, Nicholas J.; Core, Leighton J.; Lis, John T.

2014-01-01

Transcription regulation occurs frequently through promoter-associated pausing of RNA polymerase II (Pol II). We developed a Precision nuclear Run-On and sequencing assay (PRO-seq) to map the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution. Pol II accumulates immediately downstream of promoters, at intron-exon junctions that are efficiently used for splicing, and over 3' poly-adenylation sites. Focused analyses of promoters reveal that pausing is not fixed relative to initiation sites nor is it specified directly by the position of a particular core promoter element or the first nucleosome. Core promoter elements function beyond initiation, and when optimally positioned they act collectively to dictate the position and strength of pausing. We test this ‘Complex Interaction’ model with insertional mutagenesis of the Drosophila Hsp70 core promoter. PMID:23430654

Evaluating nuclear physics inputs in core-collapse supernova models

NASA Astrophysics Data System (ADS)

Lentz, E.; Hix, W. R.; Baird, M. L.; Messer, O. E. B.; Mezzacappa, A.

Core-collapse supernova models depend on the details of the nuclear and weak interaction physics inputs just as they depend on the details of the macroscopic physics (transport, hydrodynamics, etc.), numerical methods, and progenitors. We present preliminary results from our ongoing comparison studies of nuclear and weak interaction physics inputs to core collapse supernova models using the spherically-symmetric, general relativistic, neutrino radiation hydrodynamics code Agile-Boltztran. We focus on comparisons of the effects of the nuclear EoS and the effects of improving the opacities, particularly neutrino--nucleon interactions.

Effect of bisecting GlcNAc and core fucosylation on conformational properties of biantennary complex-type N-glycans in solution.

PubMed

Nishima, Wataru; Miyashita, Naoyuki; Yamaguchi, Yoshiki; Sugita, Yuji; Re, Suyong

2012-07-26

The introduction of bisecting GlcNAc and core fucosylation in N-glycans is essential for fine functional regulation of glycoproteins. In this paper, the effect of these modifications on the conformational properties of N-glycans is examined at the atomic level by performing replica-exchange molecular dynamics (REMD) simulations. We simulate four biantennary complex-type N-glycans, namely, unmodified, two single-substituted with either bisecting GlcNAc or core fucose, and disubstituted forms. By using REMD as an enhanced sampling technique, five distinct conformers in solution, each of which is characterized by its local orientation of the Manα1-6Man glycosidic linkage, are observed for all four N-glycans. The chemical modifications significantly change their conformational equilibria. The number of major conformers is reduced from five to two and from five to four upon the introduction of bisecting GlcNAc and core fucosylation, respectively. The population change is attributed to specific inter-residue hydrogen bonds, including water-mediated ones. The experimental NMR data, including nuclear Overhauser enhancement and scalar J-coupling constants, are well reproduced taking the multiple conformers into account. Our structural model supports the concept of "conformer selection", which emphasizes the conformational flexibility of N-glycans in protein-glycan interactions.

Core Physics and Kinetics Calculations for the Fissioning Plasma Core Reactor

NASA Technical Reports Server (NTRS)

Butler, C.; Albright, D.

2007-01-01

Highly efficient, compact nuclear reactors would provide high specific impulse spacecraft propulsion. This analysis and numerical simulation effort has focused on the technical feasibility issues related to the nuclear design characteristics of a novel reactor design. The Fissioning Plasma Core Reactor (FPCR) is a shockwave-driven gaseous-core nuclear reactor, which uses Magneto Hydrodynamic effects to generate electric power to be used for propulsion. The nuclear design of the system depends on two major calculations: core physics calculations and kinetics calculations. Presently, core physics calculations have concentrated on the use of the MCNP4C code. However, initial results from other codes such as COMBINE/VENTURE and SCALE4a. are also shown. Several significant modifications were made to the ISR-developed QCALC1 kinetics analysis code. These modifications include testing the state of the core materials, an improvement to the calculation of the material properties of the core, the addition of an adiabatic core temperature model and improvement of the first order reactivity correction model. The accuracy of these modifications has been verified, and the accuracy of the point-core kinetics model used by the QCALC1 code has also been validated. Previously calculated kinetics results for the FPCR were described in the ISR report, "QCALC1: A code for FPCR Kinetics Model Feasibility Analysis" dated June 1, 2002.

NUCLEAR REACTOR CORE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Preece, G.E.; Bell, F.R.; Page, R.W.

1963-03-01

A nuclear reactor core is described. It contains fuel in the form of blocks or pellets that have a grooved, wrinkled, or corrugated surface to provide a greater radiating surface area. The surfaces of spaces in the core are correspondingly corrugated for maximum heat exchange area. (C.E.S.)

LeMMINGs - I. The eMERLIN legacy survey of nearby galaxies. 1.5-GHz parsec-scale radio structures and cores

NASA Astrophysics Data System (ADS)

Baldi, R. D.; Williams, D. R. A.; McHardy, I. M.; Beswick, R. J.; Argo, M. K.; Dullo, B. T.; Knapen, J. H.; Brinks, E.; Muxlow, T. W. B.; Aalto, S.; Alberdi, A.; Bendo, G. J.; Corbel, S.; Evans, R.; Fenech, D. M.; Green, D. A.; Klöckner, H.-R.; Körding, E.; Kharb, P.; Maccarone, T. J.; Martí-Vidal, I.; Mundell, C. G.; Panessa, F.; Peck, A. B.; Pérez-Torres, M. A.; Saikia, D. J.; Saikia, P.; Shankar, F.; Spencer, R. E.; Stevens, I. R.; Uttley, P.; Westcott, J.

2018-05-01

We present the first data release of high-resolution (≤0.2 arcsec) 1.5-GHz radio images of 103 nearby galaxies from the Palomar sample, observed with the eMERLIN array, as part of the LeMMINGs survey. This sample includes galaxies which are active (low-ionization nuclear emission-line regions [LINER] and Seyfert) and quiescent (H II galaxies and absorption line galaxies, ALGs), which are reclassified based upon revised emission-line diagrams. We detect radio emission ≳0.2 mJy for 47/103 galaxies (22/34 for LINERS, 4/4 for Seyferts, 16/51 for H II galaxies, and 5/14 for ALGs) with radio sizes typically of ≲100 pc. We identify the radio core position within the radio structures for 41 sources. Half of the sample shows jetted morphologies. The remaining half shows single radio cores or complex morphologies. LINERs show radio structures more core-brightened than Seyferts. Radio luminosities of the sample range from 1032 to 1040 erg s-1: LINERs and H II galaxies show the highest and lowest radio powers, respectively, while ALGs and Seyferts have intermediate luminosities. We find that radio core luminosities correlate with black hole (BH) mass down to ˜107 M⊙, but a break emerges at lower masses. Using [O III] line luminosity as a proxy for the accretion luminosity, active nuclei and jetted H II galaxies follow an optical Fundamental Plane of BH activity, suggesting a common disc-jet relationship. In conclusion, LINER nuclei are the scaled-down version of FR I radio galaxies; Seyferts show less collimated jets; H II galaxies may host weak active BHs and/or nuclear star-forming cores; and recurrent BH activity may account for ALG properties.

Analysis of C/E results of fission rate ratio measurements in several fast lead VENUS-F cores

NASA Astrophysics Data System (ADS)

Kochetkov, Anatoly; Krása, Antonín; Baeten, Peter; Vittiglio, Guido; Wagemans, Jan; Bécares, Vicente; Bianchini, Giancarlo; Fabrizio, Valentina; Carta, Mario; Firpo, Gabriele; Fridman, Emil; Sarotto, Massimo

2017-09-01

During the GUINEVERE FP6 European project (2006-2011), the zero-power VENUS water-moderated reactor was modified into VENUS-F, a mock-up of a lead cooled fast spectrum system with solid components that can be operated in both critical and subcritical mode. The Fast Reactor Experiments for hybrid Applications (FREYA) FP7 project was launched in 2011 to support the designs of the MYRRHA Accelerator Driven System (ADS) and the ALFRED Lead Fast Reactor (LFR). Three VENUS-F critical core configurations, simulating the complex MYRRHA core design and one configuration devoted to the LFR ALFRED core conditions were investigated in 2015. The MYRRHA related cores simulated step by step design peculiarities like the BeO reflector and in pile sections. For all of these cores the fuel assemblies were of a simple design consisting of 30% enriched metallic uranium, lead rodlets to simulate the coolant and Al2O3 rodlets to simulate the oxide fuel. Fission rate ratios of minor actinides such as Np-237, Am-241 as well as Pu-239, Pu-240, Pu-242 and U-238 to U-235 were measured in these VENUS-F critical assemblies with small fission chambers in specially designed locations, to determine the spectral indices in the different neutron spectrum conditions. The measurements have been analyzed using advanced computational tools including deterministic and stochastic codes and different nuclear data sets like JEFF-3.1, JEFF-3.2, ENDF/B7.1 and JENDL-4.0. The analysis of the C/E discrepancies will help to improve the nuclear data in the specific energy region of fast neutron reactor spectra.

IMPROVEMENTS RELATING TO NUCLEAR REACTOR CORE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bell, F.R.

1963-03-01

A nuclear reactor core composed of a number of stacked horizontal layers is described. Each layer is made up of elements of moderator material of equal height and of generally hexagonal cross-section. Each element has holes containing nuclear fuel and separate ones for coolant. (C.E.S.)

Simulation numerique de l'effet du reflecteur radial sur les cellules rep en utilisant les codes DRAGON et DONJON

NASA Astrophysics Data System (ADS)

Bejaoui, Najoua

The pressurized water nuclear reactors (PWRs) is the largest fleet of nuclear reactors in operation around the world. Although these reactors have been studied extensively by designers and operators using efficient numerical methods, there are still some calculation weaknesses, given the geometric complexity of the core, still unresolved such as the analysis of the neutron flux's behavior at the core-reflector interface. The standard calculation scheme is a two steps process. In the first step, a detailed calculation at the assembly level with reflective boundary conditions, provides homogenized cross-sections for the assemblies, condensed to a reduced number of groups; this step is called the lattice calculation. The second step uses homogenized properties in each assemblies to calculate reactor properties at the core level. This step is called the full-core calculation or whole-core calculation. This decoupling of the two calculation steps is the origin of methodological bias particularly at the interface core reflector: the periodicity hypothesis used to calculate cross section librairies becomes less pertinent for assemblies that are adjacent to the reflector generally represented by these two models: thus the introduction of equivalent reflector or albedo matrices. The reflector helps to slowdown neutrons leaving the reactor and returning them to the core. This effect leads to two fission peaks in fuel assemblies localised at the core/reflector interface, the fission rate increasing due to the greater proportion of reentrant neutrons. This change in the neutron spectrum arises deep inside the fuel located on the outskirts of the core. To remedy this we simulated a peripheral assembly reflected with TMI-PWR reflector and developed an advanced calculation scheme that takes into account the environment of the peripheral assemblies and generate equivalent neutronic properties for the reflector. This scheme is tested on a core without control mechanisms and charged with fresh fuel. The results of this study showed that explicit representation of reflector and calculation of peripheral assembly with our advanced scheme allow corrections to the energy spectrum at the core interface and increase the peripheral power by up to 12% compared with that of the reference scheme.

Testing of an Integrated Reactor Core Simulator and Power Conversion System with Simulated Reactivity Feedback

NASA Technical Reports Server (NTRS)

Bragg-Sitton, Shannon M.; Hervol, David S.; Godfroy, Thomas J.

2009-01-01

A Direct Drive Gas-Cooled (DDG) reactor core simulator has been coupled to a Brayton Power Conversion Unit (BPCU) for integrated system testing at NASA Glenn Research Center (GRC) in Cleveland, OH. This is a closed-cycle system that incorporates an electrically heated reactor core module, turbo alternator, recuperator, and gas cooler. Nuclear fuel elements in the gas-cooled reactor design are replaced with electric resistance heaters to simulate the heat from nuclear fuel in the corresponding fast spectrum nuclear reactor. The thermodynamic transient behavior of the integrated system was the focus of this test series. In order to better mimic the integrated response of the nuclear-fueled system, a simulated reactivity feedback control loop was implemented. Core power was controlled by a point kinetics model in which the reactivity feedback was based on core temperature measurements; the neutron generation time and the temperature feedback coefficient are provided as model inputs. These dynamic system response tests demonstrate the overall capability of a non-nuclear test facility in assessing system integration issues and characterizing integrated system response times and response characteristics.

Testing of an Integrated Reactor Core Simulator and Power Conversion System with Simulated Reactivity Feedback

NASA Technical Reports Server (NTRS)

Bragg-Sitton, Shannon M.; Hervol, David S.; Godfroy, Thomas J.

2010-01-01

A Direct Drive Gas-Cooled (DDG) reactor core simulator has been coupled to a Brayton Power Conversion Unit (BPCU) for integrated system testing at NASA Glenn Research Center (GRC) in Cleveland, Ohio. This is a closed-cycle system that incorporates an electrically heated reactor core module, turboalternator, recuperator, and gas cooler. Nuclear fuel elements in the gas-cooled reactor design are replaced with electric resistance heaters to simulate the heat from nuclear fuel in the corresponding fast spectrum nuclear reactor. The thermodynamic transient behavior of the integrated system was the focus of this test series. In order to better mimic the integrated response of the nuclear-fueled system, a simulated reactivity feedback control loop was implemented. Core power was controlled by a point kinetics model in which the reactivity feedback was based on core temperature measurements; the neutron generation time and the temperature feedback coefficient are provided as model inputs. These dynamic system response tests demonstrate the overall capability of a non-nuclear test facility in assessing system integration issues and characterizing integrated system response times and response characteristics.

Nuclear waste disposal utilizing a gaseous core reactor

NASA Technical Reports Server (NTRS)

Paternoster, R. R.

1975-01-01

The feasibility of a gaseous core nuclear reactor designed to produce power to also reduce the national inventories of long-lived reactor waste products through nuclear transmutation was examined. Neutron-induced transmutation of radioactive wastes is shown to be an effective means of shortening the apparent half life.

Structural characterization of recombinant IAV polymerase reveals a stable complex between viral PA-PB1 heterodimer and host RanBP5.

PubMed

Swale, Christopher; Monod, Alexandre; Tengo, Laura; Labaronne, Alice; Garzoni, Frédéric; Bourhis, Jean-Marie; Cusack, Stephen; Schoehn, Guy; Berger, Imre; Ruigrok, Rob W H; Crépin, Thibaut

2016-04-20

The genome of influenza A virus (IAV) comprises eight RNA segments (vRNA) which are transcribed and replicated by the heterotrimeric IAV RNA-dependent RNA-polymerase (RdRp). RdRp consists of three subunits (PA, PB1 and PB2) and binds both the highly conserved 3'- and 5'-ends of the vRNA segment. The IAV RdRp is an important antiviral target, but its structural mechanism has remained largely elusive to date. By applying a polyprotein strategy, we produced RdRp complexes and define a minimal human IAV RdRp core complex. We show that PA-PB1 forms a stable heterodimeric submodule that can strongly interact with 5'-vRNA. In contrast, 3'-vRNA recognition critically depends on the PB2 N-terminal domain. Moreover, we demonstrate that PA-PB1 forms a stable and stoichiometric complex with host nuclear import factor RanBP5 that can be modelled using SAXS and we show that the PA-PB1-RanPB5 complex is no longer capable of 5'-vRNA binding. Our results provide further evidence for a step-wise assembly of IAV structural components, regulated by nuclear transport mechanisms and host factor binding.

A High-Throughput Cell-Based Screen Identified a 2-[(E)-2-Phenylvinyl]-8-Quinolinol Core Structure That Activates p53

PubMed Central

Bechill, John; Zhong, Rong; Zhang, Chen; Solomaha, Elena

2016-01-01

p53 function is frequently inhibited in cancer either through mutations or by increased degradation via MDM2 and/or E6AP E3-ubiquitin ligases. Most agents that restore p53 expression act by binding MDM2 or E6AP to prevent p53 degradation. However, fewer compounds directly bind to and activate p53. Here, we identified compounds that shared a core structure that bound p53, caused nuclear localization of p53 and caused cell death. To identify these compounds, we developed a novel cell-based screen to redirect p53 degradation to the Skip-Cullin-F-box (SCF) ubiquitin ligase complex in cells expressing high levels of p53. In a multiplexed assay, we coupled p53 targeted degradation with Rb1 targeted degradation in order to identify compounds that prevented p53 degradation while not inhibiting degradation through the SCF complex or other proteolytic machinery. High-throughput screening identified several leads that shared a common 2-[(E)-2-phenylvinyl]-8-quinolinol core structure that stabilized p53. Surface plasmon resonance analysis indicated that these compounds bound p53 with a KD of 200 ± 52 nM. Furthermore, these compounds increased p53 nuclear localization and transcription of the p53 target genes PUMA, BAX, p21 and FAS in cancer cells. Although p53-null cells had a 2.5±0.5-fold greater viability compared to p53 wild type cells after treatment with core compounds, loss of p53 did not completely rescue cell viability suggesting that compounds may target both p53-dependent and p53-independent pathways to inhibit cell proliferation. Thus, we present a novel, cell-based high-throughput screen to identify a 2-[(E)-2-phenylvinyl]-8-quinolinol core structure that bound to p53 and increased p53 activity in cancer cells. These compounds may serve as anti-neoplastic agents in part by targeting p53 as well as other potential pathways. PMID:27124407

Core protein: a pleiotropic keystone in the HBV lifecycle

PubMed Central

Zlotnick, Adam; Venkatakrishnan, Balasubramanian; Tan, Zhenning; Lewellyn, Eric; Turner, William; Francis, Samson

2015-01-01

Hepatitis B Virus (HBV) is a small virus whose genome has only four open reading frames. We argue that the simplicity of the virion correlates with a complexity of functions for viral proteins. We focus on the HBV core protein (Cp), a small (183 residue) protein that self-assembles to form the viral capsid. However, its functions are a little more complicated than that. In an infected cell Cp modulates every step of the viral lifecycle. Cp is bound to nuclear viral DNA and affects its epigenetics. Cp correlates with RNA specificity. Cp assembles specifically on a reverse transcriptase-viral RNA complex or, apparently, nothing at all. Indeed Cp has been one of the model systems for investigation of virus self-assembly. Cp participates in regulation of reverse transcription. Cp signals completion of reverse transcription to support virus secretion. Cp carries both nuclear localization signals and HBV surface antigen (HBsAg) binding sites; both of these functions appear to be regulated by contents of the capsid. Cp can be targeted by antivirals -- while self-assembly is the most accessible of Cp activities, we argue that it makes sense to engage the broader spectrum of Cp function. This article forms part of a symposium in Antiviral Research on “From the discovery of the Australia antigen to the development of new curative therapies for hepatitis B: an unfinished story.” PMID:26129969

Molten salt considerations for accelerator-driven subcritical fission to close the nuclear fuel cycle

NASA Astrophysics Data System (ADS)

Sooby, Elizabeth; Adams, Marvin; Baty, Austin; Gerity, James; McIntyre, Peter; Melconian, Karie; Phongikaroon, Supathorn; Pogue, Nathaniel; Sattarov, Akhdiyor; Simpson, Michael; Tripathy, Prabhat; Tsevkov, Pavel

2013-04-01

The host salt selection, molecular modeling, physical chemistry, and processing chemistry are presented here for an accelerator-driven subcritical fission in a molten salt core (ADSMS). The core is fueled solely with the transuranics (TRU) and long-lived fission products (LFP) from used nuclear fuel. The neutronics and salt composition are optimized to destroy the transuranics by fission and the long-lived fission products by transmutation. The cores are driven by proton beams from a strong-focusing cyclotron stack. One such ADSMS system can destroy the transuranics in the used nuclear fuel produced by a 1GWe conventional reactor. It uniquely provides a method to close the nuclear fuel cycle for green nuclear energy.

Metrics for the technical performance evaluation of light water reactor accident-tolerant fuel

DOE PAGES

Bragg-Sitton, Shannon M.; Todosow, Michael; Montgomery, Robert; ...

2017-03-26

The safe, reliable, and economic operation of the nation’s nuclear power reactor fleet has always been a top priority for the nuclear industry. Continual improvement of technology, including advanced materials and nuclear fuels, remains central to the industry’s success. Enhancing the accident tolerance of light water reactors (LWRs) became a topic of serious discussion following the 2011 Great East Japan Earthquake, resulting tsunami, and subsequent damage to the Fukushima Daiichi nuclear power plant complex. The overall goal for the development of accident-tolerant fuel (ATF) for LWRs is to identify alternative fuel system technologies to further enhance the safety, competitiveness, andmore » economics of commercial nuclear power. Designed for use in the current fleet of commercial LWRs or in reactor concepts with design certifications (GEN-III+), fuels with enhanced accident tolerance would endure loss of active cooling in the reactor core for a considerably longer period of time than the current fuel system while maintaining or improving performance during normal operations. The complex multiphysics behavior of LWR nuclear fuel in the integrated reactor system makes defining specific material or design improvements difficult; as such, establishing desirable performance attributes is critical in guiding the design and development of fuels and cladding with enhanced accident tolerance. Research and development of ATF in the United States is conducted under the U.S. Department of Energy (DOE) Fuel Cycle Research and Development Advanced Fuels Campaign. The DOE is sponsoring multiple teams to develop ATF concepts within multiple national laboratories, universities, and the nuclear industry. Concepts under investigation offer both evolutionary and revolutionary changes to the current nuclear fuel system. This study summarizes the technical evaluation methodology proposed in the United States to aid in the optimization and prioritization of candidate ATF designs.« less

Nuclear reactor internals alignment configuration

DOEpatents

Gilmore, Charles B [Greensburg, PA; Singleton, Norman R [Murrysville, PA

2009-11-10

An alignment system that employs jacking block assemblies and alignment posts around the periphery of the top plate of a nuclear reactor lower internals core shroud to align an upper core plate with the lower internals and the core shroud with the core barrel. The distal ends of the alignment posts are chamfered and are closely received within notches machined in the upper core plate at spaced locations around the outer circumference of the upper core plate. The jacking block assemblies are used to center the core shroud in the core barrel and the alignment posts assure the proper orientation of the upper core plate. The alignment posts may alternately be formed in the upper core plate and the notches may be formed in top plate.

Nuclear fuel elements having a composite cladding

DOEpatents

Gordon, Gerald M.; Cowan, II, Robert L.; Davies, John H.

1983-09-20

An improved nuclear fuel element is disclosed for use in the core of nuclear reactors. The improved nuclear fuel element has a composite cladding of an outer portion forming a substrate having on the inside surface a metal layer selected from the group consisting of copper, nickel, iron and alloys of the foregoing with a gap between the composite cladding and the core of nuclear fuel. The nuclear fuel element comprises a container of the elongated composite cladding, a central core of a body of nuclear fuel material disposed in and partially filling the container and forming an internal cavity in the container, an enclosure integrally secured and sealed at each end of said container and a nuclear fuel material retaining means positioned in the cavity. The metal layer of the composite cladding prevents perforations or failures in the cladding substrate from stress corrosion cracking or from fuel pellet-cladding interaction or both. The substrate of the composite cladding is selected from conventional cladding materials and preferably is a zirconium alloy.

Do All Dinoflagellates have an Extranuclear Spindle?

PubMed

Moon, Eunyoung; Nam, Seung Won; Shin, Woongghi; Park, Myung Gil; Coats, D Wayne

2015-11-01

The syndinean dinoflagellates are a diverse assemblage of alveolate endoparasites that branch basal to the core dinoflagellates. Because of their phylogenetic position, the syndineans are considered key model microorganisms in understanding early evolution in the dinoflagellates. Closed mitosis with an extranuclear spindle that traverses the nucleus in cytoplasmic grooves or tunnels is viewed as one of the morphological features shared by syndinean and core dinoflagellates. Here we describe nuclear morphology and mitosis in the syndinean dinoflagellate Amoebophrya sp. from Akashiwo sanguinea, a member of the A. ceratii complex, as revealed by protargol silver impregnation, DNA specific fluorochromes, and transmission electron microscopy. Our observations show that not all species classified as dinoflagellates have an extranuclear spindle. In Amoebophrya sp. from A. sanguinea, an extranuclear microtubule cylinder located in a depression in the nuclear surface during interphase moves into the nucleoplasm via sequential membrane fusion events and develops into an entirely intranuclear spindle. Results suggest that the intranuclear spindle of Amoebophrya spp. may have evolved from an ancestral extranuclear spindle and indicate the need for taxonomic revision of the Amoebophryidae. Copyright © 2015 Elsevier GmbH. All rights reserved.

Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments

PubMed Central

Kozłowska, Małgorzata; Tarczewska, Aneta; Jakób, Michał; Bystranowska, Dominika; Taube, Michał; Kozak, Maciej; Czarnocki-Cieciura, Mariusz; Dziembowski, Andrzej; Orłowski, Marek; Tkocz, Katarzyna; Ożyhar, Andrzej

2017-01-01

Nucleoplasmins are a nuclear chaperone family defined by the presence of a highly conserved N-terminal core domain. X-ray crystallographic studies of isolated nucleoplasmin core domains revealed a β-propeller structure consisting of a set of five monomers that together form a stable pentamer. Recent studies on isolated N-terminal domains from Drosophila 39-kDa FK506-binding protein (FKBP39) and from other chromatin-associated proteins showed analogous, nucleoplasmin-like (NPL) pentameric structures. Here, we report that the NPL domain of the full-length FKBP39 does not form pentameric complexes. Multi-angle light scattering (MALS) and sedimentation equilibrium ultracentrifugation (SE AUC) analyses of the molecular mass of the full-length protein indicated that FKBP39 forms homotetrameric complexes. Molecular models reconstructed from small-angle X-ray scattering (SAXS) revealed that the NPL domain forms a stable, tetrameric core and that FK506-binding domains are linked to it by intrinsically disordered, flexible chains that form tentacle-like segments. Analyses of full-length FKBP39 and its isolated NPL domain suggested that the distal regions of the polypeptide chain influence and determine the quaternary conformation of the nucleoplasmin-like protein. These results provide new insights regarding the conserved structure of nucleoplasmin core domains and provide a potential explanation for the importance of the tetrameric structural organization of full-length nucleoplasmins. PMID:28074868

Nuclear design of a vapor core reactor for space nuclear propulsion

NASA Astrophysics Data System (ADS)

Dugan, Edward T.; Watanabe, Yoichi; Kuras, Stephen A.; Maya, Isaac; Diaz, Nils J.

1993-01-01

Neutronic analysis methodology and results are presented for the nuclear design of a vapor core reactor for space nuclear propulsion. The Nuclear Vapor Thermal Reactor (NVTR) Rocket Engine uses modified NERVA geometry and systems which the solid fuel replaced by uranium tetrafluoride vapor. The NVTR is an intermediate term gas core thermal rocket engine with specific impulse in the range of 1000-1200 seconds; a thrust of 75,000 lbs for a hydrogen flow rate of 30 kg/s; average core exit temperatures of 3100 K to 3400 K; and reactor thermal powers of 1400 to 1800 MW. Initial calculations were performed on epithermal NVTRs using ZrC fuel elements. Studies are now directed at thermal NVTRs that use fuel elements made of C-C composite. The large ZrC-moderated reactors resulted in thrust-to-weight ratios of only 1 to 2; the compact C-C composite systems yield thrust-to-weight ratios of 3 to 5.

Ab Initio Investigations of High-Pressure Melting of Dense Lithium

NASA Astrophysics Data System (ADS)

Clay, Raymond; Morales, Miguel; Bonev, Stanimir

Lithium at ambient conditions is the simplest alkali metal and exhibits textbook nearly-free electron behavior. As the density is increased, however, significant core/valence overlap leads to surprisingly complex chemistry. We have systematically investigated the phase diagram of lithium at pressures ranging between two and six million atmospheres. Through a combination of density functional theory based path-integral and classical molecular dynamics simulations, we have investigated the impact of both nuclear quantum effects and anharmonicity on the melting line and solid phase boundaries. We also investigate how the inclusion of nuclear quantum effects and approximations in the treatment of electronic exchange-correlation impact the robustness of previous predictions of tetrahedral clustering in dense liquid Li. Sandia National Laboratories is a multi-mission laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

Statistical methods for thermonuclear reaction rates and nucleosynthesis simulations

NASA Astrophysics Data System (ADS)

Iliadis, Christian; Longland, Richard; Coc, Alain; Timmes, F. X.; Champagne, Art E.

2015-03-01

Rigorous statistical methods for estimating thermonuclear reaction rates and nucleosynthesis are becoming increasingly established in nuclear astrophysics. The main challenge being faced is that experimental reaction rates are highly complex quantities derived from a multitude of different measured nuclear parameters (e.g., astrophysical S-factors, resonance energies and strengths, particle and γ-ray partial widths). We discuss the application of the Monte Carlo method to two distinct, but related, questions. First, given a set of measured nuclear parameters, how can one best estimate the resulting thermonuclear reaction rates and associated uncertainties? Second, given a set of appropriate reaction rates, how can one best estimate the abundances from nucleosynthesis (i.e., reaction network) calculations? The techniques described here provide probability density functions that can be used to derive statistically meaningful reaction rates and final abundances for any desired coverage probability. Examples are given for applications to s-process neutron sources, core-collapse supernovae, classical novae, and Big Bang nucleosynthesis.

Heterobimetallic Complexes with MIII-(μ-OH)-MII Cores (MIII = Fe, Mn, Ga; MII = Ca, Sr, and Ba): Structural, Kinetic, and Redox Properties

PubMed Central

Park, Young Jun; Cook, Sarah A.; Sickerman, Nathaniel S.; Sano, Yohei; Ziller, Joseph W.

2013-01-01

The effects of redox-inactive metal ions on dioxygen activation were explored using a new FeII complex containing a tripodal ligand with 3 sulfonamido groups. This iron complex exhibited a faster initial rate for the reduction of O2 than its MnII analog. Increases in initial rates were also observed in the presence of group 2 metal ions for both the FeII and MnII complexes, which followed the trend NMe4+ < BaII < CaII = SrII. These studies led to the isolation of heterobimetallic complexes containing FeIII-(μ-OH)-MII cores (MII = Ca, Sr, and Ba) and one with a [SrII(OH)MnIII]+ motif. The analogous [CaII(OH)GaIII]+ complex was also prepared and its solid state molecular structure is nearly identical to that of the [CaII(OH)FeIII]+ system. Nuclear magnetic resonance studies indicated that the diamagnetic [CaII(OH)GaIII]+ complex retained its structure in solution. Electrochemical measurements on the heterobimetallic systems revealed similar one-electron reduction potentials for the [CaII(OH)FeIII]+ and [SrII(OH)FeIII]+ complexes, which were more positive than the potential observed for [BaII(OH)FeIII]+. Similar results were obtained for the heterobimetallic MnII complexes. These findings suggest that Lewis acidity is not the only factor to consider when evaluating the effects of group 2 ions on redox processes, including those within the oxygen-evolving complex of Photosystem II. PMID:24058726

Heterobimetallic Complexes with MIII-(μ-OH)-MII Cores (MIII = Fe, Mn, Ga; MII = Ca, Sr, and Ba): Structural, Kinetic, and Redox Properties.

PubMed

Park, Young Jun; Cook, Sarah A; Sickerman, Nathaniel S; Sano, Yohei; Ziller, Joseph W; Borovik, A S

2013-02-01

The effects of redox-inactive metal ions on dioxygen activation were explored using a new Fe II complex containing a tripodal ligand with 3 sulfonamido groups. This iron complex exhibited a faster initial rate for the reduction of O 2 than its Mn II analog. Increases in initial rates were also observed in the presence of group 2 metal ions for both the Fe II and Mn II complexes, which followed the trend NMe 4 + < Ba II < Ca II = Sr II . These studies led to the isolation of heterobimetallic complexes containing Fe III -( μ -OH)-M II cores (M II = Ca, Sr, and Ba) and one with a [Sr II (OH)Mn III ] + motif. The analogous [Ca II (OH)Ga III ] + complex was also prepared and its solid state molecular structure is nearly identical to that of the [Ca II (OH)Fe III ] + system. Nuclear magnetic resonance studies indicated that the diamagnetic [Ca II (OH)Ga III ] + complex retained its structure in solution. Electrochemical measurements on the heterobimetallic systems revealed similar one-electron reduction potentials for the [Ca II (OH)Fe III ] + and [Sr II (OH)Fe III ] + complexes, which were more positive than the potential observed for [Ba II (OH)Fe III ] + . Similar results were obtained for the heterobimetallic Mn II complexes. These findings suggest that Lewis acidity is not the only factor to consider when evaluating the effects of group 2 ions on redox processes, including those within the oxygen-evolving complex of Photosystem II.

Deflection Measurements of a Thermally Simulated Nuclear Core Using a High-Resolution CCD-Camera

NASA Technical Reports Server (NTRS)

Stanojev, B. J.; Houts, M.

2004-01-01

Space fission systems under consideration for near-term missions all use compact. fast-spectrum reactor cores. Reactor dimensional change with increasing temperature, which affects neutron leakage. is the dominant source of reactivity feedback in these systems. Accurately measuring core dimensional changes during realistic non-nuclear testing is therefore necessary in predicting the system nuclear equivalent behavior. This paper discusses one key technique being evaluated for measuring such changes. The proposed technique is to use a Charged Couple Device (CCD) sensor to obtain deformation readings of electrically heated prototypic reactor core geometry. This paper introduces a technique by which a single high spatial resolution CCD camera is used to measure core deformation in Real-Time (RT). Initial system checkout results are presented along with a discussion on how additional cameras could be used to achieve a three- dimensional deformation profile of the core during test.

Fluorescent protein-tagged Vpr dissociates from HIV-1 core after viral fusion and rapidly enters the cell nucleus.

PubMed

Desai, Tanay M; Marin, Mariana; Sood, Chetan; Shi, Jiong; Nawaz, Fatima; Aiken, Christopher; Melikyan, Gregory B

2015-10-29

HIV-1 Vpr is recruited into virions during assembly and appears to remain associated with the viral core after the reverse transcription and uncoating steps of entry. This feature has prompted the use of fluorescently labeled Vpr to visualize viral particles and to follow trafficking of post-fusion HIV-1 cores in the cytoplasm. Here, we tracked single pseudovirus entry and fusion and observed that fluorescently tagged Vpr gradually dissociates from post-fusion viral cores over the course of several minutes and accumulates in the nucleus. Kinetics measurements showed that fluorescent Vpr released from the cores very rapidly entered the cell nucleus. More than 10,000 Vpr molecules can be delivered into the cell nucleus within 45 min of infection by HIV-1 particles pseudotyped with the avian sarcoma and leukosis virus envelope glycoprotein. The fraction of Vpr from cell-bound viruses that accumulated in the nucleus was proportional to the extent of virus-cell fusion and was fully blocked by viral fusion inhibitors. Entry of virus-derived Vpr into the nucleus occurred independently of envelope glycoproteins or target cells. Fluorescence correlation spectroscopy revealed two forms of nuclear Vpr-monomers and very large complexes, likely involving host factors. The kinetics of viral Vpr entering the nucleus after fusion was not affected by point mutations in the capsid protein that alter the stability of the viral core. The independence of Vpr shedding of capsid stability and its relatively rapid dissociation from post-fusion cores suggest that this process may precede capsid uncoating, which appears to occur on a slower time scale. Our results thus demonstrate that a bulk of fluorescently labeled Vpr incorporated into HIV-1 particles is released shortly after fusion. Future studies will address the question whether the quick and efficient nuclear delivery of Vpr derived from incoming viruses can regulate subsequent steps of HIV-1 infection.

Comprehensive proteomic analysis of the human spliceosome

NASA Astrophysics Data System (ADS)

Zhou, Zhaolan; Licklider, Lawrence J.; Gygi, Steven P.; Reed, Robin

2002-09-01

The precise excision of introns from pre-messenger RNA is performed by the spliceosome, a macromolecular machine containing five small nuclear RNAs and numerous proteins. Much has been learned about the protein components of the spliceosome from analysis of individual purified small nuclear ribonucleoproteins and salt-stable spliceosome `core' particles. However, the complete set of proteins that constitutes intact functional spliceosomes has yet to be identified. Here we use maltose-binding protein affinity chromatography to isolate spliceosomes in highly purified and functional form. Using nanoscale microcapillary liquid chromatography tandem mass spectrometry, we identify ~145 distinct spliceosomal proteins, making the spliceosome the most complex cellular machine so far characterized. Our spliceosomes comprise all previously known splicing factors and 58 newly identified components. The spliceosome contains at least 30 proteins with known or putative roles in gene expression steps other than splicing. This complexity may be required not only for splicing multi-intronic metazoan pre-messenger RNAs, but also for mediating the extensive coupling between splicing and other steps in gene expression.

Feasibility study of full-reactor gas core demonstration test

NASA Technical Reports Server (NTRS)

Kunze, J. F.; Lofthouse, J. H.; Shaffer, C. J.; Macbeth, P. J.

1973-01-01

Separate studies of nuclear criticality, flow patterns, and thermodynamics for the gas core reactor concept have all given positive indications of its feasibility. However, before serious design for a full scale gas core application can be made, feasibility must be shown for operation with full interaction of the nuclear, thermal, and hydraulic effects. A minimum sized, and hence minimum expense, test arrangement is considered for a full gas core configuration. It is shown that the hydrogen coolant scattering effects dominate the nuclear considerations at elevated temperatures. A cavity diameter of somewhat larger than 4 ft (122 cm) will be needed if temperatures high enough to vaporize uranium are to be achieved.

Proteomic Analysis of the Multimeric Nuclear Egress Complex of Human Cytomegalovirus*

PubMed Central

Milbradt, Jens; Kraut, Alexandra; Hutterer, Corina; Sonntag, Eric; Schmeiser, Cathrin; Ferro, Myriam; Wagner, Sabrina; Lenac, Tihana; Claus, Claudia; Pinkert, Sandra; Hamilton, Stuart T.; Rawlinson, William D.; Sticht, Heinrich; Couté, Yohann; Marschall, Manfred

2014-01-01

Herpesviral capsids are assembled in the host cell nucleus before being translocated into the cytoplasm for further maturation. The crossing of the nuclear envelope represents a major event that requires the formation of the nuclear egress complex (NEC). Previous studies demonstrated that human cytomegalovirus (HCMV) proteins pUL50 and pUL53, as well as their homologs in all members of Herpesviridae, interact with each other at the nuclear envelope and form the heterodimeric core of the NEC. In order to characterize further the viral and cellular protein content of the multimeric NEC, the native complex was isolated from HCMV-infected human primary fibroblasts at various time points and analyzed using quantitative proteomics. Previously postulated components of the HCMV-specific NEC, as well as novel potential NEC-associated proteins such as emerin, were identified. In this regard, interaction and colocalization between emerin and pUL50 were confirmed by coimmunoprecipitation and confocal microscopy analyses, respectively. A functional validation of viral and cellular NEC constituents was achieved through siRNA-mediated knockdown experiments. The important role of emerin in NEC functionality was demonstrated by a reduction of viral replication when emerin expression was down-regulated. Moreover, under such conditions, reduced production of viral proteins and deregulation of viral late cytoplasmic maturation were observed. Combined, these data prove the functional importance of emerin as an NEC component, associated with pUL50, pUL53, pUL97, p32/gC1qR, and further regulatory proteins. Summarized, our findings provide the first proteomics-based characterization and functional validation of the HCMV-specific multimeric NEC. PMID:24969177

Solid0Core Heat-Pipe Nuclear Batterly Type Reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ehud Greenspan

This project was devoted to a preliminary assessment of the feasibility of designing an Encapsulated Nuclear Heat Source (ENHS) reactor to have a solid core from which heat is removed by liquid-metal heat pipes (HP).

Comparison of ENDF/B-VII.1 and JEFF-3.2 in VVER-1000 operational data calculation

NASA Astrophysics Data System (ADS)

Frybort, Jan

2017-09-01

Safe operation of a nuclear reactor requires an extensive calculational support. Operational data are determined by full-core calculations during the design phase of a fuel loading. Loading pattern and design of fuel assemblies are adjusted to meet safety requirements and optimize reactor operation. Nodal diffusion code ANDREA is used for this task in case of Czech VVER-1000 reactors. Nuclear data for this diffusion code are prepared regularly by lattice code HELIOS. These calculations are conducted in 2D on fuel assembly level. There is also possibility to calculate these macroscopic data by Monte-Carlo Serpent code. It can make use of alternative evaluated libraries. All calculations are affected by inherent uncertainties in nuclear data. It is useful to see results of full-core calculations based on two sets of diffusion data obtained by Serpent code calculations with ENDF/B-VII.1 and JEFF-3.2 nuclear data including also decay data library and fission yields data. The comparison is based directly on fuel assembly level macroscopic data and resulting operational data. This study illustrates effect of evaluated nuclear data library on full-core calculations of a large PWR reactor core. The level of difference which results exclusively from nuclear data selection can help to understand the level of inherent uncertainties of such full-core calculations.

Nuclear cardiology core syllabus of the European Association of Cardiovascular Imaging (EACVI).

PubMed

Gimelli, Alessia; Neglia, Danilo; Schindler, Thomas H; Cosyns, Bernard; Lancellotti, Patrizio; Kitsiou, Anastasia

2015-04-01

The European Association of Cardiovascular Imaging (EACVI) Core Syllabus for Nuclear Cardiology is now available online. The syllabus lists key elements of knowledge in nuclear cardiology. It represents a framework for the development of training curricula and provides expected knowledge-based learning outcomes to the nuclear cardiology trainees. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2015. For permissions please email: journals.permissions@oup.com.

Realistic Gamow shell model for resonance and continuum in atomic nuclei

NASA Astrophysics Data System (ADS)

Xu, F. R.; Sun, Z. H.; Wu, Q.; Hu, B. S.; Dai, S. J.

2018-02-01

The Gamow shell model can describe resonance and continuum for atomic nuclei. The model is established in the complex-moment (complex-k) plane of the Berggren coordinates in which bound, resonant and continuum states are treated on equal footing self-consistently. In the present work, the realistic nuclear force, CD Bonn, has been used. We have developed the full \\hat{Q}-box folded-diagram method to derive the realistic effective interaction in the model space which is nondegenerate and contains resonance and continuum channels. The CD-Bonn potential is renormalized using the V low-k method. With choosing 16O as the inert core, we have applied the Gamow shell model to oxygen isotopes.

Square lattice honeycomb reactor for space power and propulsion

NASA Astrophysics Data System (ADS)

Gouw, Reza; Anghaie, Samim

2000-01-01

The most recent nuclear design study at the Innovative Nuclear Space Power and Propulsion Institute (INSPI) is the Moderated Square-Lattice Honeycomb (M-SLHC) reactor design utilizing the solid solution of ternary carbide fuels. The reactor is fueled with solid solution of 93% enriched (U,Zr,Nb)C. The square-lattice honeycomb design provides high strength and is amenable to the processing complexities of these ultrahigh temperature fuels. The optimum core configuration requires a balance between high specific impulse and thrust level performance, and maintaining the temperature and strength limits of the fuel. The M-SLHC design is based on a cylindrical core that has critical radius and length of 37 cm and 50 cm, respectively. This design utilized zirconium hydrate to act as moderator. The fuel sub-assemblies are designed as cylindrical tubes with 12 cm in diameter and 10 cm in length. Five fuel subassemblies are stacked up axially to form one complete fuel assembly. These fuel assemblies are then arranged in the circular arrangement to form two fuel regions. The first fuel region consists of six fuel assemblies, and 18 fuel assemblies for the second fuel region. A 10-cm radial beryllium reflector in addition to 10-cm top axial beryllium reflector is used to reduce neutron leakage from the system. To perform nuclear design analysis of the M-SLHC design, a series of neutron transport and diffusion codes are used. To optimize the system design, five axial regions are specified. In each axial region, temperature and fuel density are varied. The axial and radial power distributions for the system are calculated, as well as the axial and radial flux distributions. Temperature coefficients of the system are also calculated. A water submersion accident scenario is also analyzed for these systems. Results of the nuclear design analysis indicate that a compact core can be designed based on ternary uranium carbide square-lattice honeycomb fuel, which provides a relatively high thrust to weight ratio. .

CORE (Common Operating Response Environment) Software Technology Suite

ScienceCinema

Gelston, Gariann; Rohlfing, Kerrie

2018-05-30

Agencies that oversee complex, multi-stakeholder programs need efficient, secure ways to link people and knowledge within and across organizations. The Common Operating Response Environment (CORE), a software suite developed by PNNL researchers does just that. The CORE tool—which is customizable for a multitude of uses—facilitates situational awareness by integrating diverse data streams without the need to reformat them, summarizing that information, and providing users with the information they need to rapidly understand and appropriately respond to situations. It is mobile device-ready, has a straightforward interface for ease of use across organizations and skill sets, and is incredibly configurable to the needs of each specific user, whether they require data summaries for high-level decision makers or tactical maps, operational data, or weather information for responders in the field. Information can be input into CORE and queried in a variety of ways—using customized forms, reports, visuals, or other organizational templates—according to the needs of each user’s organization, teams, and business processes. CORE data forms, for instance, could be accessed and used in real-time to capture information about vessels being inspected for nuclear material.

A multi-locus analysis of phylogenetic relationships within grass subfamily Pooideae (Poaceae) inferred from sequences of nuclear single copy gene regions compared with plastid DNA.

PubMed

Hochbach, Anne; Schneider, Julia; Röser, Martin

2015-06-01

To investigate phylogenetic relationships within the grass subfamily Pooideae we studied about 50 taxa covering all recognized tribes, using one plastid DNA (cpDNA) marker (matK gene-3'trnK exon) and for the first time four nuclear single copy gene loci. DNA sequence information from two parts of the nuclear genes topoisomerase 6 (Topo6) spanning the exons 8-13 and 17-19, the exons 9-13 encoding plastid acetyl-CoA-carboxylase (Acc1) and the partial exon 1 of phytochrome B (PhyB) were generated. Individual and nuclear combined data were evaluated using maximum parsimony, maximum likelihood and Bayesian methods. All of the phylogenetic results show Brachyelytrum and the tribe Nardeae as earliest diverging lineages within the subfamily. The 'core' Pooideae (Hordeeae and the Aveneae/Poeae tribe complex) are also strongly supported, as well as the monophyly of the tribes Brachypodieae, Meliceae and Stipeae (except PhyB). The beak grass tribe Diarrheneae and the tribe Duthieeae are not monophyletic in some of the analyses. However, the combined nuclear DNA (nDNA) tree yields the highest resolution and the best delimitation of the tribes, and provides the following evolutionary hypothesis for the tribes: Brachyelytrum, Nardeae, Duthieeae, Meliceae, Stipeae, Diarrheneae, Brachypodieae and the 'core' Pooideae. Within the individual datasets, the phylogenetic trees obtained from Topo6 exon 8-13 shows the most interesting results. The divergent positions of some clone sequences of Ampelodesmos mauritanicus and Trikeraia pappiformis, for instance, may indicate a hybrid origin of these stipoid taxa. Copyright © 2015 Elsevier Inc. All rights reserved.

Optimize out-of-core thermionic energy conversion for nuclear electric propulsion

NASA Technical Reports Server (NTRS)

Morris, J. F.

1977-01-01

Current designs for out of core thermionic energy conversion (TEC) to power nuclear electric propulsion (NEP) were evaluated. Approaches to improve out of core TEC are emphasized and probabilities for success are indicated. TEC gains are available with higher emitter temperatures and greater power densities. Good potentialities for accommodating external high temperature, high power density TEC with heat pipe cooled reactors exist.

Impacts of Vehicle (In)Security

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chugg, J.; Rohde, K.

Nuclear and radioactive material is routinely transported worldwide every day. Since 2010, the complexity of the transport vehicle to support such activities has grown exponentially. Many core functions of a vehicle are now handled by small embedded computer modules with more being added each year to enhance the owner’s experience and convenience. With a system as complex as today’s automobile, the potential for cyber security issues is certain. Hackers have begun exploring this new domain with public information increasingly disseminated. Because vehicles are allowed into and around secure nuclear facilities, the potential for using a vehicle as a new cybermore » entry point or vector into the facility is now plausible and must be mitigated. In addition, compromising such a vehicle could aide in illicit removal of nuclear material, putting sensitive cargo at risk. Because cyber attacks can now be introduced using vehicles, cyber security, needs to be integrated into an organization’s design basis threat document. Essentially, a vehicle now extends the perimeter for which security professionals are responsible.Electronic Control Units (ECU) responsible for handling all core and ancillary vehicle functions are interconnected using the controller area network (CAN) bus. A typical CAN network in a modern automobile contains 50 or more ECUs. The CAN protocol now supports a wide variety of areas, including automotive, road transportation, rail transportation, industrial automation, power generation, maritime, military vehicles, aviation, and medical devices. In many ways, the nuclear industry is employing the CAN bus protocol or other similar broadcast serial networks. This paper will provide an overview of the current state of automobile and CAN Bus security, as well as an overview of what has been publicly disclosed by many research organizations. It will then present several hypotheses of how vehicle security issues may impact nuclear activities. An initial discussion of how a vehicle can be used as a new threat vector to penetrate secure facilities will be presented. This includes how a modern automobile can be used as the exploitation mechanism for nearby devices such as laptops, cell phones, and wireless access points. Additional discussion will highlight how vehicle security might impact transportation of nuclear material through remote exploitation of a moving vehicle. The final discussion will include what possible implications might be relative to the physical protection systems at nuclear facilities. The audience will also be given details regarding the complexity of attack, thus implying the likelihood of successful exploitation, and information on how such attacks may be mitigated. Emerging security products for automobiles will be discussed and other mitigation methods will be detailed (e.g. disabling vehicle cellular modems). As a result, the audience will have a greater understanding of how to add vehicle security as a part of a comprehensive nuclear security policy.Finally, this paper will highlight the similarities between CAN Bus and other broadcast serial bus networks such as Profibus or DeviceNet, helping educate the reader on how susceptible this type of networking is to nefarious attacks and how it might affect components connected to many different nuclear systems, including control systems, safety systems, emergency systems, and support systems.« less

ACCUMULATION OF PHOTOSYSTEM ONE1, a Member of a Novel Gene Family, Is Required for Accumulation of [4Fe-4S] Cluster–Containing Chloroplast Complexes and Antenna Proteins

PubMed Central

Amann, Katrin; Lezhneva, Lina; Wanner, Gerd; Herrmann, Reinhold G.; Meurer, Jörg

2004-01-01

To investigate the nuclear-controlled mechanisms of [4Fe-4S] cluster assembly in chloroplasts, we selected Arabidopsis thaliana mutants with a decreased content of photosystem I (PSI) containing three [4Fe-4S] clusters. One identified gene, ACCUMULATION OF PHOTOSYSTEM ONE1 (APO1), belongs to a previously unknown gene family with four defined groups (APO1 to APO4) only found in nuclear genomes of vascular plants. All homologs contain two related motifs of ∼100 amino acid residues that could potentially provide ligands for [4Fe-4S] clusters. APO1 is essentially required for photoautotrophic growth, and levels of PSI core subunits are below the limit of detection in the apo1 mutant. Unlike other Arabidopsis PSI mutants, apo1 fails to accumulate significant amounts of the outer antenna subunits of PSI and PSII and to form grana stacks. In particular, APO1 is essentially required for stable accumulation of other plastid-encoded and nuclear-encoded [4Fe-4S] cluster complexes within the chloroplast, whereas [2Fe-2S] cluster–containing complexes appear to be unaffected. In vivo labeling experiments and analyses of polysome association suggest that translational elongation of the PSI transcripts psaA and psaB is specifically arrested in the mutant. Taken together, our findings suggest that APO1 is involved in the stable assembly of several [4Fe-4S] cluster–containing complexes of chloroplasts and interferes with translational events probably in association with plastid nucleoids. PMID:15494558

System Design for a Nuclear Electric Spacecraft Utilizing Out-of-core Thermionic Conversion

NASA Technical Reports Server (NTRS)

Estabrook, W. C.; Phillips, W. M.; Hsieh, T.

1976-01-01

Basic guidelines are presented for a nuclear space power system which utilizes heat pipes to transport thermal power from a fast nuclear reactor to an out of core thermionic converter array. Design parameters are discussed for the nuclear reactor, heat pipes, thermionic converters, shields (neutron and gamma), waste heat rejection systems, and the electrical bus bar-cable system required to transport the high current/low voltage power to the processing equipment. Dimensions are compatible with shuttle payload bay constraints.

Update and evaluation of decay data for spent nuclear fuel analyses

NASA Astrophysics Data System (ADS)

Simeonov, Teodosi; Wemple, Charles

2017-09-01

Studsvik's approach to spent nuclear fuel analyses combines isotopic concentrations and multi-group cross-sections, calculated by the CASMO5 or HELIOS2 lattice transport codes, with core irradiation history data from the SIMULATE5 reactor core simulator and tabulated isotopic decay data. These data sources are used and processed by the code SNF to predict spent nuclear fuel characteristics. Recent advances in the generation procedure for the SNF decay data are presented. The SNF decay data includes basic data, such as decay constants, atomic masses and nuclide transmutation chains; radiation emission spectra for photons from radioactive decay, alpha-n reactions, bremsstrahlung, and spontaneous fission, electrons and alpha particles from radioactive decay, and neutrons from radioactive decay, spontaneous fission, and alpha-n reactions; decay heat production; and electro-atomic interaction data for bremsstrahlung production. These data are compiled from fundamental (ENDF, ENSDF, TENDL) and processed (ESTAR) sources for nearly 3700 nuclides. A rigorous evaluation procedure of internal consistency checks and comparisons to measurements and benchmarks, and code-to-code verifications is performed at the individual isotope level and using integral characteristics on a fuel assembly level (e.g., decay heat, radioactivity, neutron and gamma sources). Significant challenges are presented by the scope and complexity of the data processing, a dearth of relevant detailed measurements, and reliance on theoretical models for some data.

Structural materials for Gen-IV nuclear reactors: Challenges and opportunities

NASA Astrophysics Data System (ADS)

Murty, K. L.; Charit, I.

2008-12-01

Generation-IV reactor design concepts envisioned thus far cater toward a common goal of providing safer, longer lasting, proliferation-resistant and economically viable nuclear power plants. The foremost consideration in the successful development and deployment of Gen-IV reactor systems is the performance and reliability issues involving structural materials for both in-core and out-of-core applications. The structural materials need to endure much higher temperatures, higher neutron doses and extremely corrosive environment, which are beyond the experience of the current nuclear power plants. Materials under active consideration for use in different reactor components include various ferritic/martensitic steels, austenitic stainless steels, nickel-base superalloys, ceramics, composites, etc. This paper presents a summary of various Gen-IV reactor concepts, with emphasis on the structural materials issues depending on the specific application areas. This paper also discusses the challenges involved in using the existing materials under both service and off-normal conditions. Tasks become increasingly complex due to the operation of various fundamental phenomena like radiation-induced segregation, radiation-enhanced diffusion, precipitation, interactions between impurity elements and radiation-produced defects, swelling, helium generation and so forth. Further, high temperature capability (e.g. creep properties) of these materials is a critical, performance-limiting factor. It is demonstrated that novel alloy and microstructural design approaches coupled with new materials processing and fabrication techniques may mitigate the challenges, and the optimum system performance may be achieved under much demanding conditions.

Core Versus Nuclear Gauge Methods of Determining Soil Bulk Density and Moisture Content

Treesearch

Jacqueline G. Steele; Jerry L. Koger; Albert C. Trouse; Donald L. Sirois

1983-01-01

Soil bulk and moisture content measurements were obtained using two nuclear gauge systems and those compared to those obtained from soil cores. The soils, a Hiwassee sandy loam, a Lakeland loamy sand, and a Loyd clay, were free of organic matter and uniform in mechanical composition. The regression equations developed for the nuclear guages for the first phase of the...

Method for automatically scramming a nuclear reactor

DOEpatents

Ougouag, Abderrafi M.; Schultz, Richard R.; Terry, William K.

2005-12-27

An automatically scramming nuclear reactor system. One embodiment comprises a core having a coolant inlet end and a coolant outlet end. A cooling system operatively associated with the core provides coolant to the coolant inlet end and removes heated coolant from the coolant outlet end, thus maintaining a pressure differential therebetween during a normal operating condition of the nuclear reactor system. A guide tube is positioned within the core with a first end of the guide tube in fluid communication with the coolant inlet end of the core, and a second end of the guide tube in fluid communication with the coolant outlet end of the core. A control element is positioned within the guide tube and is movable therein between upper and lower positions, and automatically falls under the action of gravity to the lower position when the pressure differential drops below a safe pressure differential.

Fissioning uranium plasmas and nuclear-pumped lasers

NASA Technical Reports Server (NTRS)

Schneider, R. T.; Thom, K.

1975-01-01

Current research into uranium plasmas, gaseous-core (cavity) reactors, and nuclear-pumped lasers is discussed. Basic properties of fissioning uranium plasmas are summarized together with potential space and terrestrial applications of gaseous-core reactors and nuclear-pumped lasers. Conditions for criticality of a uranium plasma are outlined, and it is shown that the nonequilibrium state and the optical thinness of a fissioning plasma can be exploited for the direct conversion of fission fragment energy into coherent light (i.e., for nuclear-pumped lasers). Successful demonstrations of nuclear-pumped lasers are described together with gaseous-fuel reactor experiments using uranium hexafluoride.

Inorganic biochemistry with short-lived radioisotopes as nuclear probes

NASA Astrophysics Data System (ADS)

Tröger, W.; Butz, T.

2000-12-01

Metal ions are ubiquitous in the biosphere. In living organisms metalloproteins with specifically designed metal cores perform vital chemical processes. On the other hand, several heavy metals are detrimental to living organisms and nature has developed effective enzymatic detoxification systems which convert toxic metal ions to less toxic species. The nuclear spectroscopy technique Time Differential Perturbed Angular Correlation (TDPAC) of γ-rays uses radioactive isotopes as nuclear probes in these metal cores to obtain a better understanding of the structural and functional significance of these metal cores by monitoring the nuclear quadrupole interaction of the TDPAC probe. Since this technique is based on the nuclear decay, it is also applicable under physiological conditions, i.e., especially at picomolar concentrations. For these studies an indispensable prerequisite is the production of the TDPAC probes with highest possible specific activity and purity as is done by the on-line mass separator ISOLDE at CERN in Geneva.

Cytosolic iron chaperones: Proteins delivering iron cofactors in the cytosol of mammalian cells.

PubMed

Philpott, Caroline C; Ryu, Moon-Suhn; Frey, Avery; Patel, Sarju

2017-08-04

Eukaryotic cells contain hundreds of metalloproteins that are supported by intracellular systems coordinating the uptake and distribution of metal cofactors. Iron cofactors include heme, iron-sulfur clusters, and simple iron ions. Poly(rC)-binding proteins are multifunctional adaptors that serve as iron ion chaperones in the cytosolic/nuclear compartment, binding iron at import and delivering it to enzymes, for storage (ferritin) and export (ferroportin). Ferritin iron is mobilized by autophagy through the cargo receptor, nuclear co-activator 4. The monothiol glutaredoxin Glrx3 and BolA2 function as a [2Fe-2S] chaperone complex. These proteins form a core system of cytosolic iron cofactor chaperones in mammalian cells. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Promyelocytic Leukemia (Pml) Nuclear Bodies Are Protein Structures That Do Not Accumulate RNA

PubMed Central

Boisvert, François-Michel; Hendzel, Michael J.; Bazett-Jones, David P.

2000-01-01

The promyelocytic leukemia (PML) nuclear body (also referred to as ND10, POD, and Kr body) is involved in oncogenesis and viral infection. This subnuclear domain has been reported to be rich in RNA and a site of nascent RNA synthesis, implicating its direct involvement in the regulation of gene expression. We used an analytical transmission electron microscopic method to determine the structure and composition of PML nuclear bodies and the surrounding nucleoplasm. Electron spectroscopic imaging (ESI) demonstrates that the core of the PML nuclear body is a dense, protein-based structure, 250 nm in diameter, which does not contain detectable nucleic acid. Although PML nuclear bodies contain neither chromatin nor nascent RNA, newly synthesized RNA is associated with the periphery of the PML nuclear body, and is found within the chromatin-depleted region of the nucleoplasm immediately surrounding the core of the PML nuclear body. We further show that the RNA does not accumulate in the protein core of the structure. Our results dismiss the hypothesis that the PML nuclear body is a site of transcription, but support the model in which the PML nuclear body may contribute to the formation of a favorable nuclear environment for the expression of specific genes. PMID:10648561

Role of nuclear reactions on stellar evolution of intermediate-mass stars

NASA Astrophysics Data System (ADS)

Möller, H.; Jones, S.; Fischer, T.; Martínez-Pinedo, G.

2018-01-01

The evolution of intermediate-mass stars (8 - 12 solar masses) represents one of the most challenging subjects in nuclear astrophysics. Their final fate is highly uncertain and strongly model dependent. They can become white dwarfs, they can undergo electron-capture or core-collapse supernovae or they might even proceed towards explosive oxygen burning and a subsequent thermonuclear explosion. We believe that an accurate description of nuclear reactions is crucial for the determination of the pre-supernova structure of these stars. We argue that due to the possible development of an oxygen-deflagration, a hydrodynamic description has to be used. We implement a nuclear reaction network with ∼200 nuclear species into the implicit hydrodynamic code AGILE. The reaction network considers all relevant nuclear electron captures and beta-decays. For selected relevant nuclear species, we include a set of updated reaction rates, for which we discuss the role for the evolution of the stellar core, at the example of selected stellar models. We find that the final fate of these intermediate-mass stars depends sensitively on the density threshold for weak processes that deleptonize the core.

Constraints on the symmetry energy from neutron star observations

NASA Astrophysics Data System (ADS)

Newton, W. G.; Gearheart, M.; Wen, De-Hua; Li, Bao-An

2013-03-01

The modeling of many neutron star observables incorporates the microphysics of both the stellar crust and core, which is tied intimately to the properties of the nuclear matter equation of state (EoS). We explore the predictions of such models over the range of experimentally constrained nuclear matter parameters, focusing on the slope of the symmetry energy at nuclear saturation density L. We use a consistent model of the composition and EoS of neutron star crust and core matter to model the binding energy of pulsar B of the double pulsar system J0737-3039, the frequencies of torsional oscillations of the neutron star crust and the instability region for r-modes in the neutron star core damped by electron-electron viscosity at the crust-core interface. By confronting these models with observations, we illustrate the potential of astrophysical observables to offer constraints on poorly known nuclear matter parameters complementary to terrestrial experiments, and demonstrate that our models consistently predict L < 70 MeV.

Thermal Hydraulics Design and Analysis Methodology for a Solid-Core Nuclear Thermal Rocket Engine Thrust Chamber

NASA Technical Reports Server (NTRS)

Wang, Ten-See; Canabal, Francisco; Chen, Yen-Sen; Cheng, Gary; Ito, Yasushi

2013-01-01

Nuclear thermal propulsion is a leading candidate for in-space propulsion for human Mars missions. This chapter describes a thermal hydraulics design and analysis methodology developed at the NASA Marshall Space Flight Center, in support of the nuclear thermal propulsion development effort. The objective of this campaign is to bridge the design methods in the Rover/NERVA era, with a modern computational fluid dynamics and heat transfer methodology, to predict thermal, fluid, and hydrogen environments of a hypothetical solid-core, nuclear thermal engine the Small Engine, designed in the 1960s. The computational methodology is based on an unstructured-grid, pressure-based, all speeds, chemically reacting, computational fluid dynamics and heat transfer platform, while formulations of flow and heat transfer through porous and solid media were implemented to describe those of hydrogen flow channels inside the solid24 core. Design analyses of a single flow element and the entire solid-core thrust chamber of the Small Engine were performed and the results are presented herein

Plant nuclei can contain extensive grooves and invaginations

NASA Technical Reports Server (NTRS)

Collings, D. A.; Carter, C. N.; Rink, J. C.; Scott, A. C.; Wyatt, S. E.; Allen, N. S.; Brown, C. S. (Principal Investigator)

2000-01-01

Plant cells can exhibit highly complex nuclear organization. Through dye-labeling experiments in untransformed onion epidermal and tobacco culture cells and through the expression of green fluorescent protein targeted to either the nucleus or the lumen of the endoplasmic reticulum/nuclear envelope in these cells, we have visualized deep grooves and invaginations into the large nuclei of these cells. In onion, these structures, which are similar to invaginations seen in some animal cells, form tubular or planelike infoldings of the nuclear envelope. Both grooves and invaginations are stable structures, and both have cytoplasmic cores containing actin bundles that can support cytoplasmic streaming. In dividing tobacco cells, invaginations seem to form during cell division, possibly from strands of the endoplasmic reticulum trapped in the reforming nucleus. The substantial increase in nuclear surface area resulting from these grooves and invaginations, their apparent preference for association with nucleoli, and the presence in them of actin bundles that support vesicle motility suggest that the structures might function both in mRNA export from the nucleus and in protein import from the cytoplasm to the nucleus.

Plant Nuclei Can Contain Extensive Grooves and InvaginationsW⃞W⃞

PubMed Central

Collings, David A.; Carter, Crystal N.; Rink, Jochen C.; Scott, Amie C.; Wyatt, Sarah E.; Allen, Nina Strömgren

2000-01-01

Plant cells can exhibit highly complex nuclear organization. Through dye-labeling experiments in untransformed onion epidermal and tobacco culture cells and through the expression of green fluorescent protein targeted to either the nucleus or the lumen of the endoplasmic reticulum/nuclear envelope in these cells, we have visualized deep grooves and invaginations into the large nuclei of these cells. In onion, these structures, which are similar to invaginations seen in some animal cells, form tubular or planelike infoldings of the nuclear envelope. Both grooves and invaginations are stable structures, and both have cytoplasmic cores containing actin bundles that can support cytoplasmic streaming. In dividing tobacco cells, invaginations seem to form during cell division, possibly from strands of the endoplasmic reticulum trapped in the reforming nucleus. The substantial increase in nuclear surface area resulting from these grooves and invaginations, their apparent preference for association with nucleoli, and the presence in them of actin bundles that support vesicle motility suggest that the structures might function both in mRNA export from the nucleus and in protein import from the cytoplasm to the nucleus. PMID:11148288

Nuclear fuel element

DOEpatents

Armijo, Joseph S.; Coffin, Jr., Louis F.

1980-04-29

A nuclear fuel element for use in the core of a nuclear reactor is disclosed and has an improved composite cladding comprised of a moderate purity metal barrier of zirconium metallurgically bonded on the inside surface of a zirconium alloy tube. The metal barrier forms a shield between the alloy tube and a core of nuclear fuel material enclosed in the composite cladding. There is a gap between the cladding and the core. The metal barrier forms about 1 to about 30 percent of the thickness of the composite cladding and has low neutron absorption characteristics. The metal barrier serves as a preferential reaction site for gaseous impurities and fission products and protects the alloy tube from contact and reaction with such impurities and fission products. Methods of manufacturing the composite cladding are also disclosed.

Nuclear reactor downcomer flow deflector

DOEpatents

Gilmore, Charles B [Greensburg, PA; Altman, David A [Pittsburgh, PA; Singleton, Norman R [Murrysville, PA

2011-02-15

A nuclear reactor having a coolant flow deflector secured to a reactor core barrel in line with a coolant inlet nozzle. The flow deflector redirects incoming coolant down an annulus between the core barrel and the reactor vessel. The deflector has a main body with a front side facing the fluid inlet nozzle and a rear side facing the core barrel. The rear side of the main body has at least one protrusion secured to the core barrel so that a gap exists between the rear side of the main body adjacent the protrusion and the core barrel. Preferably, the protrusion is a relief that circumscribes the rear side of the main body.

HIGH TEMPERATURE, HIGH POWER HETEROGENEOUS NUCLEAR REACTOR

DOEpatents

Hammond, R.P.; Wykoff, W.R.; Busey, H.M.

1960-06-14

A heterogeneous nuclear reactor is designed comprising a stationary housing and a rotatable annular core being supported for rotation about a vertical axis in the housing, the core containing a plurality of radial fuel- element supporting channels, the cylindrical empty space along the axis of the core providing a central plenum for the disposal of spent fuel elements, the core cross section outer periphery being vertically gradated in radius one end from the other to provide a coolant duct between the core and the housing, and means for inserting fresh fuel elements in the supporting channels under pressure and while the reactor is in operation.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Galat, Andrzej, E-mail: galat@dsvidf.cea.fr; Thai, Robert

Highlights: • The hFKBP25 interacts with diverse components of macromolecular entities. • We show that the endogenous human FKBP25 is bound to polyribosomes. • The endogenous hFKBP25 co-immunoprecipitated with nucleosomal proteins. • FKBP25 could induce conformational switch in macromolecular complexes. - Abstract: In this paper, we show some evidence that a member of the FK506-binding proteins, FKBP25 is associated to diverse components that are part of several different intracellular large-molecular mass entities. The FKBP25 is a high-affinity rapamycin-binding immunophilin, which has nuclear translocation signals present in its PPIase domain but it was detected both in the cytoplasm compartment and inmore » the nuclear proteome. Analyses of antiFKBP25-immunoprecipitated proteins have revealed that the endogenous FKBP25 is associated to the core histones of the nucleosome, and with several proteins forming spliceosomal complexes and ribosomal subunits. Using polyclonal antiFKBP25 we have detected FKBP25 associated with polyribosomes. Added RNAs or 0.5 M NaCl release FKBP25 that was associated with the polyribosomes indicating that the immunophilin has an intrinsic capacity to form complexes with polyribonucleotides via its charged surface patches. Rapamycin or FK506 treatments of the polyribosomes isolated from porcine brain, HeLa and K568 cells caused a residual release of the endogenous FKBP25, which suggests that the immunophilin also binds to some proteins via its PPIase cavity. Our proteomics study indicates that the nuclear pool of the FKBP25 targets various nuclear proteins that are crucial for packaging of DNA, chromatin remodeling and pre-mRNA splicing whereas the cytosolic pool of this immunophilin is bound to some components of the ribosome.« less

Design and implementation of a simple nuclear power plant simulator

NASA Astrophysics Data System (ADS)

Miller, William H.

1983-02-01

A simple PWR nuclear power plant simulator has been designed and implemented on a minicomputer system. The system is intended for students use in understanding the power operation of a nuclear power plant. A PDP-11 minicomputer calculates reactor parameters in real time, uses a graphics terminal to display the results and a keyboard and joystick for control functions. Plant parameters calculated by the model include the core reactivity (based upon control rod positions, soluble boron concentration and reactivity feedback effects), the total core power, the axial core power distribution, the temperature and pressure in the primary and secondary coolant loops, etc.

Integrated Modeling and Experiments to Characterize Coupled Thermo-hydro-geomechanical-chemical processes in Hydraulic Fracturing

NASA Astrophysics Data System (ADS)

Viswanathan, H. S.; Carey, J. W.; Karra, S.; Porter, M. L.; Rougier, E.; Kang, Q.; Makedonska, N.; Hyman, J.; Jimenez Martinez, J.; Frash, L.; Chen, L.

2015-12-01

Hydraulic fracturing phenomena involve fluid-solid interactions embedded within coupled thermo-hydro-mechanical-chemical (THMC) processes over scales from microns to tens of meters. Feedbacks between processes result in complex dynamics that must be unraveled if one is to predict and, in the case of unconventional resources, facilitate fracture propagation, fluid flow, and interfacial transport processes. The proposed work is part of a broader class of complex systems involving coupled fluid flow and fractures that are critical to subsurface energy issues, such as shale oil, geothermal, carbon sequestration, and nuclear waste disposal. We use unique LANL microfluidic and triaxial core flood experiments integrated with state-of-the-art numerical simulation to reveal the fundamental dynamics of fracture-fluid interactions to characterize the key coupled processes that impact hydrocarbon production. We are also comparing CO2-based fracturing and aqueous fluids to enhance production, greatly reduce waste water, while simultaneously sequestering CO2. We will show pore, core and reservoir scale simulations/experiments that investigate the contolling mechanisms that control hydrocarbon production.

NUCLEAR REACTOR

DOEpatents

Treshow, M.

1961-09-01

A boiling-water nuclear reactor is described wherein control is effected by varying the moderator-to-fuel ratio in the reactor core. This is accomplished by providing control tubes containing a liquid control moderator in the reactor core and providing means for varying the amount of control moderatcr within the control tubes.

Quenching behavior of molten pool with different strategies – A review

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shrikant,, E-mail: 2014rmt9018@mnit.ac.in; Pandel, U.; Duchaniya, R. K.

After the major severe accident in nuclear reactor, there has been lot of concerns regarding long term core melt stabilization following a severe accident in nuclear reactors. Numerous strategies have been though for quenching and stabilization of core melt like top flooding, bottom flooding, indirect cooling, etc. However, the effectiveness of these schemes is yet to be determined properly, for which, lot of experiments are needed. Several experiments have been performed for coolability of melt pool under bottom flooding as well as for indirect cooling. Besides these tests are very scattered because they involve different simulants material initial temperatures andmore » masses of melt, which makes it very complex to judge the effectiveness of a particular technique and advantage over the other. In this review paper, a study has been carried on different cooling techniques of simulant materials with same mass. Three techniques have been compared here and the results are discussed. Under top flooding technique it took several hours to cool the melt under without decay heat condition. In bottom flooding technique was found to be the best technique among in indirect cooling technique, top flooded technique, and bottom flooded technique.« less

Nuclear core positioning system

DOEpatents

Garkisch, Hans D.; Yant, Howard W.; Patterson, John F.

1979-01-01

A structural support system for the core of a nuclear reactor which achieves relatively restricted clearances at operating conditions and yet allows sufficient clearance between fuel assemblies at refueling temperatures. Axially displaced spacer pads having variable between pad spacing and a temperature compensated radial restraint system are utilized to maintain clearances between the fuel elements. The core support plates are constructed of metals specially chosen such that differential thermal expansion produces positive restraint at operating temperatures.

Fission control system for nuclear reactor

DOEpatents

Conley, G.H.; Estes, G.P.

Control system for nuclear reactor comprises a first set of reactivity modifying rods fixed in a reactor core with their upper ends stepped in height across the core, and a second set of reactivity modifying rods movable vertically within the reactor core and having their lower ends stepped to correspond with the stepped arrangement of the first set of rods, pairs of the rods of the first and second sets being in coaxial alignment.

Assessment of multiple sources of anthropogenic and natural chemical inputs to a morphologically complex basin, Lake Mead, USA

USGS Publications Warehouse

Rosen, Michael R.; Van Metre, P.C.

2010-01-01

Lakes with complex morphologies and with different geologic and land-use characteristics in their sub-watersheds could have large differences in natural and anthropogenic chemical inputs to sub-basins in the lake. Lake Mead in southern Nevada and northern Arizona, USA, is one such lake. To assess variations in chemical histories from 1935 to 1998 for major sub-basins of Lake Mead, four sediment cores were taken from three different parts of the reservoir (two from Las Vegas Bay and one from the Overton Arm and Virgin Basin) and analyzed for major and trace elements, radionuclides, and organic compounds. As expected, anthropogenic contaminant inputs are greatest to Las Vegas Bay reflecting inputs from the Las Vegas urban area, although concentrations are low compared to sediment quality guidelines and to other USA lakes. One exception to this pattern was higher Hg in the Virgin Basin core. The Virgin Basin core is located in the main body of the lake (Colorado River channel) and is influenced by the hydrology of the Colorado River, which changed greatly with completion of Glen Canyon Dam upstream in 1963. Major and trace elements in the core show pronounced shifts in the early 1960s and, in many cases, gradually return to concentrations more typical of pre-1960s by the 1980s and 1990s, after the filling of Lake Powell. The Overton Arm is the sub-basin least effected by anthropogenic contaminant inputs but has a complex 137Cs profile with a series of large peaks and valleys over the middle of the core, possibly reflecting fallout from nuclear tests in the 1950s at the Nevada Test Site. The 137Cs profile suggests a much greater sedimentation rate during testing which we hypothesize results from greatly increased dust fall on the lake and Virgin and Muddy River watersheds. The severe drought in the southwestern USA during the 1950s might also have played a role in variations in sedimentation rate in all of the cores. ?? 2009.

Essential Ingredients in Core-collapse Supernovae

DOE PAGES

Hix, William Raphael; Lentz, E. J.; Endeve, Eirik; ...

2014-03-27

Marking the inevitable death of a massive star, and the birth of a neutron star or black hole, core-collapse supernovae bring together physics at a wide range in spatial scales, from kilometer-sized hydrodynamic motions (eventually growing to gigameter scale) down to femtometer scale nuclear reactions. Carrying 10more » $$^{44}$$ joules of kinetic energy and a rich-mix of newly synthesized atomic nuclei, core-collapse supernovae are the preeminent foundries of the nuclear species which make up ourselves and our solar system. We will discuss our emerging understanding of the convectively unstable, neutrino-driven explosion mechanism, based on increasingly realistic neutrino-radiation hydrodynamic simulations that include progressively better nuclear and particle physics. Recent multi-dimensional models with spectral neutrino transport from several research groups, which slowly develop successful explosions for a range of progenitors, have motivated changes in our understanding of the neutrino reheating mechanism. In a similar fashion, improvements in nuclear physics, most notably explorations of weak interactions on nuclei and the nuclear equation of state, continue to refine our understanding of how supernovae explode. Recent progress on both the macroscopic and microscopic effects that affect core-collapse supernovae are discussed.« less

High-Frequency Fe-H Vibrations in a Bridging Hydride Complex Characterized by NRVS and DFT.

PubMed

Pelmenschikov, Vladimir; Gee, Leland B; Wang, Hongxin; MacLeod, K Cory; McWilliams, Sean F; Skubi, Kazimer L; Cramer, Stephen P; Holland, Patrick L

2018-05-30

High-spin iron species with bridging hydrides have been detected in species trapped during nitrogenase catalysis, but there are few general methods of evaluating Fe-H bonds in high-spin multinuclear iron systems. An 57 Fe nuclear resonance vibrational spectroscopy (NRVS) study on an Fe(μ-H) 2 Fe model complex reveals Fe-H stretching vibrations for bridging hydrides at frequencies greater than 1200 cm -1 . These isotope-sensitive vibrational bands are not evident in infrared (IR) spectra, showing the power of NRVS for identifying hydrides in this high-spin iron system. Complementary density functional theory (DFT) calculations elucidate the normal modes of the rhomboidal iron hydride core. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Benton, J; Wall, D; Parker, E

This paper presents the latest information on one of the Accelerated Highly Enriched Uranium (HEU) Disposition initiatives that resulted from the May 2002 Summit meeting between Presidents George W. Bush and Vladimir V. Putin. These initiatives are meant to strengthen nuclear nonproliferation objectives by accelerating the disposition of nuclear weapons-useable materials. The HEU Transparency Implementation Program (TIP), within the National Nuclear Security Administration (NNSA) is working to implement one of the selected initiatives that would purchase excess Russian HEU (93% 235U) for use as fuel in U.S. research reactors over the next ten years. This will parallel efforts to convertmore » the reactors' fuel core from HEU to low enriched uranium (LEU) material, where feasible. The paper will examine important aspects associated with the U.S. research reactor HEU purchase. In particular: (1) the establishment of specifications for the Russian HEU, and (2) transportation safeguard considerations for moving the HEU from the Mayak Production Facility in Ozersk, Russia, to the Y-12 National Security Complex in Oak Ridge, TN.« less

Chromatin Insulators: A Role in Nuclear Organization and Gene Expression

PubMed Central

Yang, Jingping; Corces, Victor G.

2011-01-01

Chromatin insulators are DNA-protein complexes with broad functions in nuclear biology. Based on the ability of insulator proteins to interact with each other, it was originally thought that insulators form loops that could constitute functional domains of co-regulated gene expression. Nevertheless, data from genome-wide localization studies indicate that insulator proteins can be present in intergenic regions as well as at the 5′, introns or 3′ of genes, suggesting a broader role in chromosome biology. Cells have developed mechanisms to control insulator activity by recruiting specialized proteins or by covalent modification of core components. Recent results suggest that insulators mediate intra- and inter-chromosomal interactions to affect transcription, imprinting and recombination. It is possible that these interactions set up cell-specific blueprints of nuclear organization that may contribute to the establishment of different patterns of gene expression during cell differentiation. As a consequence, disruption of insulator activity could result in the development of cancer or other disease states. PMID:21704228

Spring design for use in the core of a nuclear reactor

DOEpatents

Willard, Jr., H. James

1993-01-01

A spring design particularly suitable for use in the core of a nuclear reactor includes one surface having a first material oriented in a longitudinal direction, and another surface having a second material oriented in a transverse direction. The respective surfaces exhibit different amounts of irraditation induced strain.

Liquid level, void fraction, and superheated steam sensor for nuclear-reactor cores. [PWR; BWR

DOEpatents

Tokarz, R.D.

1981-10-27

This disclosure relates to an apparatus for monitoring the presence of coolant in liquid or mixed liquid and vapor, and superheated gaseous phases at one or more locations within an operating nuclear reactor core, such as pressurized water reactor or a boiling water reactor.

Preliminary analysis of loss-of-coolant accident in Fukushima nuclear accident

DOE Office of Scientific and Technical Information (OSTI.GOV)

Su'ud, Zaki; Anshari, Rio

Loss-of-Coolant Accident (LOCA) in Boiling Water Reactor (BWR) especially on Fukushima Nuclear Accident will be discussed in this paper. The Tohoku earthquake triggered the shutdown of nuclear power reactors at Fukushima Nuclear Power station. Though shutdown process has been completely performed, cooling process, at much smaller level than in normal operation, is needed to remove decay heat from the reactor core until the reactor reach cold-shutdown condition. If LOCA happen at this condition, it will cause the increase of reactor fuel and other core temperatures and can lead to reactor core meltdown and exposure of radioactive material to the environmentmore » such as in the Fukushima Dai Ichi nuclear accident case. In this study numerical simulation has been performed to calculate pressure composition, water level and temperature distribution on reactor during this accident. There are two coolant regulating system that operational on reactor unit 1 at this accident, Isolation Condensers (IC) system and Safety Relief Valves (SRV) system. Average mass flow of steam to the IC system in this event is 10 kg/s and could keep reactor core from uncovered about 3,2 hours and fully uncovered in 4,7 hours later. There are two coolant regulating system at operational on reactor unit 2, Reactor Core Isolation Condenser (RCIC) System and Safety Relief Valves (SRV). Average mass flow of coolant that correspond this event is 20 kg/s and could keep reactor core from uncovered about 73 hours and fully uncovered in 75 hours later. There are three coolant regulating system at operational on reactor unit 3, Reactor Core Isolation Condenser (RCIC) system, High Pressure Coolant Injection (HPCI) system and Safety Relief Valves (SRV). Average mass flow of water that correspond this event is 15 kg/s and could keep reactor core from uncovered about 37 hours and fully uncovered in 40 hours later.« less

Preliminary analysis of loss-of-coolant accident in Fukushima nuclear accident

NASA Astrophysics Data System (ADS)

Su'ud, Zaki; Anshari, Rio

2012-06-01

Loss-of-Coolant Accident (LOCA) in Boiling Water Reactor (BWR) especially on Fukushima Nuclear Accident will be discussed in this paper. The Tohoku earthquake triggered the shutdown of nuclear power reactors at Fukushima Nuclear Power station. Though shutdown process has been completely performed, cooling process, at much smaller level than in normal operation, is needed to remove decay heat from the reactor core until the reactor reach cold-shutdown condition. If LOCA happen at this condition, it will cause the increase of reactor fuel and other core temperatures and can lead to reactor core meltdown and exposure of radioactive material to the environment such as in the Fukushima Dai Ichi nuclear accident case. In this study numerical simulation has been performed to calculate pressure composition, water level and temperature distribution on reactor during this accident. There are two coolant regulating system that operational on reactor unit 1 at this accident, Isolation Condensers (IC) system and Safety Relief Valves (SRV) system. Average mass flow of steam to the IC system in this event is 10 kg/s and could keep reactor core from uncovered about 3,2 hours and fully uncovered in 4,7 hours later. There are two coolant regulating system at operational on reactor unit 2, Reactor Core Isolation Condenser (RCIC) System and Safety Relief Valves (SRV). Average mass flow of coolant that correspond this event is 20 kg/s and could keep reactor core from uncovered about 73 hours and fully uncovered in 75 hours later. There are three coolant regulating system at operational on reactor unit 3, Reactor Core Isolation Condenser (RCIC) system, High Pressure Coolant Injection (HPCI) system and Safety Relief Valves (SRV). Average mass flow of water that correspond this event is 15 kg/s and could keep reactor core from uncovered about 37 hours and fully uncovered in 40 hours later.

Scope Complexity Options Risks Excursions (SCORE) Version 3.0 Mathematical Description.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gearhart, Jared Lee; Samberson, Jonell Nicole; Shettigar, Subhasini

The purpose of the Scope, Complexity, Options, Risks, Excursions (SCORE) model is to estimate the relative complexity of design variants of future warhead options. The results of this model allow those considering these options to understand the complexity tradeoffs between proposed warhead options. The core idea of SCORE is to divide a warhead option into a well- defined set of scope elements and then estimate the complexity of each scope element against a well understood reference system. The uncertainty associated with estimates can also be captured. A weighted summation of the relative complexity of each scope element is used tomore » determine the total complexity of the proposed warhead option or portions of the warhead option (i.e., a National Work Breakdown Structure code). The SCORE analysis process is a growing multi-organizational Nuclear Security Enterprise (NSE) effort, under the management of the NA- 12 led Enterprise Modeling and Analysis Consortium (EMAC), that has provided the data elicitation, integration and computation needed to support the out-year Life Extension Program (LEP) cost estimates included in the Stockpile Stewardship Management Plan (SSMP).« less

Space nuclear reactors — A post-operational disposal strategy

NASA Astrophysics Data System (ADS)

Angelo, Joseph A.; Buden, David

If 100-kWe and multimegawatt-electric class space nuclear reactors are to play a significant role in humanity's push into cislunar and heliocentric space in the next millennium, the obvious advantages of space nuclear power plants should not be denied to space mission planners due to a failure to develop internationally-acceptable post-operational disposal strategies for spent reactor cores. This is true whether the space reactor has shut down at the end of its normal mission lifetime or in response to an onboard system failure/emergency which causes a premature mission termination. Up until now the great majority of aerospace nuclear safety efforts have concentrated on prelaunch, launch and reactor startup activities. In fact, with the exception of the development of the "nuclear safe orbit" (NSO) concept, little technical attention has yet been given to the post-operational disposal of future space reactors. This paper describes the technical alternatives available for the safe, acceptable disposal of space reactors that could be used in a wide variety of space applications in the 21st Century. Post-operational core radioactivity levels for typical advanced design (hundred kWe-class) space reactors are presented as a function of decay time and contrasted to the spent core radionuclide inventory of the SNAP-10A system, the only nuclear reactor operated in space by the United States. The role of a permanent space station, smart robotic systems, and an operating lunar base in support of spent core disposal strategies is also presented, including use of a selected portion of the lunar surface as an internationally-designated spent reactor core repository.

Multiple Core Galaxies: Implications for M31

NASA Technical Reports Server (NTRS)

Smith, B. F.; Miller, R. H.; Cuzzi, Jeffrey N. (Technical Monitor)

1994-01-01

It is generally perceived that two cores cannot survive very long within the nuclear regions of a galaxy. The recent HST discovery of a double nucleus in M31 brings this question into prominence. Physical conditions in the nuclear regions of a typical galaxy help a second core survive so it can orbit for a long time, possibly for thousands of orbits. Given the nearly uniform mass density in a core, tidal forces within a core radius are compressive in all directions and help the core survive the buffeting it takes as it orbits near the center of the galaxy. We use numerical experiments to illustrate these physical principles. Modifications to the experimental method allow the full power of the experiments to be concentrated on the nuclear regions. Spatial resolution of about 0.2 parsec comfortably resolves detail within the 1.4 parsec core radius of the second, but brighter, core (P1) in M31. The same physical principles apply in other astronomical situations, such as dumbbell galaxies, galaxies orbiting near the center of a galaxy cluster, and subclustering in galaxy clusters. The experiments also illustrate that galaxy encounters and merging are quite sensitive to external tidal forces, such as those produced by the gravitational potential in a group or cluster of galaxies.

Substructure of the inner core of the Earth.

PubMed Central

Herndon, J M

1996-01-01

The rationale is disclosed for a substructure within the Earth's inner core, consisting of an actinide subcore at the center of the Earth, surrounded by a subshell composed of the products of nuclear fission and radioactive decay. Estimates are made as to possible densities, physical dimensions, and chemical compositions. The feasibility for self-sustaining nuclear fission within the subcore is demonstrated, and implications bearing on the structure and geodynamic activity of the inner core are discussed. PMID:11607625

Photoenergy Harvesting Organic PV Cells Using Modified Photosynthetic Light-Harvesting Complex for Energy Harvesting Materials

DTIC Science & Technology

2008-07-03

complex is still unclear even in the crystal structure of RC-LH1 core complex from Rhodopseudomonas (Rps.) palustris [1]. In this study, we use a...complex of R. palustris . 16 The NIR absorption spectra of these core complexes on the electrode indicate that these complexes are stable when...as the LH or the core complex. For example, the core complex, isolated from the photosynthetic bacterium, Rps. palustris , was successfully

Emergency heat removal system for a nuclear reactor

DOEpatents

Dunckel, Thomas L.

1976-01-01

A heat removal system for nuclear reactors serving as a supplement to an Emergency Core Cooling System (ECCS) during a Loss of Coolant Accident (LOCA) comprises a plurality of heat pipes having one end in heat transfer relationship with either the reactor pressure vessel, the core support grid structure or other in-core components and the opposite end located in heat transfer relationship with a heat exchanger having heat transfer fluid therein. The heat exchanger is located external to the pressure vessel whereby excessive core heat is transferred from the above reactor components and dissipated within the heat exchanger fluid.

Determination of the neutron activation profile of core drill samples by gamma-ray spectrometry.

PubMed

Gurau, D; Boden, S; Sima, O; Stanga, D

2018-04-01

This paper provides guidance for determining the neutron activation profile of core drill samples taken from the biological shield of nuclear reactors using gamma spectrometry measurements. Thus, it provides guidance for selecting a model of the right form to fit data and using least squares methods for model fitting. The activity profiles of two core samples taken from the biological shield of a nuclear reactor were determined. The effective activation depth and the total activity of core samples along with their uncertainties were computed by Monte Carlo simulation. Copyright © 2017 Elsevier Ltd. All rights reserved.

Nature and morphology of fumed oxides and features of interfacial phenomena

NASA Astrophysics Data System (ADS)

Gun'ko, V. M.; Zarko, V. I.; Goncharuk, O. V.; Matkovsky, A. K.; Remez, O. S.; Skubiszewska-Zięba, J.; Wojcik, G.; Walusiak, B.; Blitz, J. P.

2016-03-01

Individual and complex fumed nanooxides were studied using high-resolution transmission electron microscopy, X-ray diffraction, ultraviolet-visible (UV-vis) spectroscopy, differential scanning calorimetry, nuclear magnetic resonance spectroscopy, adsorption, desorption (evaporation), and quantum chemical methods. For mixed nanooxides in contrast to simple and small nanoparticles of individual silica or titania, complex core-shell nanoparticles (50-200 nm in size) with titania or alumina cores and silica or alumina shells can be destroyed under high-pressure cryogelation (HPCG), mechnochemical activation (MCA) that also affect the structure of aggregates of nanoparticles and agglomerates of aggregates becoming more compacted. This is accompanied by changes in color from white to beige of different tints and changes in the UV-vis spectra in the 300-600 nm range, as well as changes in crystalline structure of alumina. Any treatment of 'soft' nanooxides affects the interfacial behavior of polar and nonpolar adsorbates. For some of them, the hysteresis loops become strongly open. Rearrangement of secondary particles affects the freezing-melting point depression. Clusterization of adsorbates bound in pores causes diminution of heat effects during phase transition (freezing, fusion). Freezing point depression and increasing melting point cause significant hysteresis freezing-melting effects for adsorbates bound to oxide nanoparticles. The study shows that complex nanooxides can be more sensitive to external actions than simple nanooxides such as silica.

Fuel handling system for a nuclear reactor

DOEpatents

Saiveau, James G.; Kann, William J.; Burelbach, James P.

1986-01-01

A pool type nuclear fission reactor has a core, with a plurality of core elements and a redan which confines coolant as a hot pool at a first end of the core separated from a cold pool at a second end of the core by the redan. A fuel handling system for use with such reactors comprises a core element storage basket located outside of the redan in the cold pool. An access passage is formed in the redan with a gate for opening and closing the passage to maintain the temperature differential between the hot pool and the cold pool. A mechanism is provided for opening and closing the gate. A lifting arm is also provided for manipulating the fuel core elements through the access passage between the storage basket and the core when the redan gate is open.

Fuel handling system for a nuclear reactor

DOEpatents

Saiveau, James G.; Kann, William J.; Burelbach, James P.

1986-12-02

A pool type nuclear fission reactor has a core, with a plurality of core elements and a redan which confines coolant as a hot pool at a first end of the core separated from a cold pool at a second end of the core by the redan. A fuel handling system for use with such reactors comprises a core element storage basket located outside of the redan in the cold pool. An access passage is formed in the redan with a gate for opening and closing the passage to maintain the temperature differential between the hot pool and the cold pool. A mechanism is provided for opening and closing the gate. A lifting arm is also provided for manipulating the fuel core elements through the access passage between the storage basket and the core when the redan gate is open.

Solution structure of the core SMN–Gemin2 complex

PubMed Central

Sarachan, Kathryn L.; Valentine, Kathleen G.; Gupta, Kushol; Moorman, Veronica R.; Gledhill, John M.; Bernens, Matthew; Tommos, Cecilia; Wand, A. Joshua; Van Duyne, Gregory D.

2012-01-01

In humans, assembly of spliceosomal snRNPs (small nuclear ribonucleoproteins) begins in the cytoplasm where the multi-protein SMN (survival of motor neuron) complex mediates the formation of a seven-membered ring of Sm proteins on to a conserved site of the snRNA (small nuclear RNA). The SMN complex contains the SMN protein Gemin2 and several additional Gemins that participate in snRNP biosynthesis. SMN was first identified as the product of a gene found to be deleted or mutated in patients with the neurodegenerative disease SMA (spinal muscular atrophy), the leading genetic cause of infant mortality. In the present study, we report the solution structure of Gemin2 bound to the Gemin2-binding domain of SMN determined by NMR spectroscopy. This complex reveals the structure of Gemin2, how Gemin2 binds to SMN and the roles of conserved SMN residues near the binding interface. Surprisingly, several conserved SMN residues, including the sites of two SMA patient mutations, are not required for binding to Gemin2. Instead, they form a conserved SMN/Gemin2 surface that may be functionally important for snRNP assembly. The SMN–Gemin2 structure explains how Gemin2 is stabilized by SMN and establishes a framework for structure–function studies to investigate snRNP biogenesis as well as biological processes involving Gemin2 that do not involve snRNP assembly. PMID:22607171

Prevent, Counter, and Respond - A Strategic Plan to Reduce Global Nuclear Threats (FY 2016-FY2020)

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2015-03-01

NNSA’s second core mission is reducing global nuclear dangers by preventing the acquisition of nuclear weapons or weapons-usable materials, countering efforts to acquire such weapons or materials, and responding to nuclear or radiological incidents. In 2015, NNSA reorganized its nonproliferation activities based on core competencies and realigned its counterterrorism and counterproliferation functions to more efficiently address both current and emerging threats and challenges. The reorganization accompanied the March 2015 release of the first ever Prevent, Counter, and Respond – A Strategic Plan to Reduce Global Nuclear Threats. This report, which NNSA will update annually, highlights key nuclear threat trends andmore » describes NNSA’s integrated threat reduction strategy.« less

Exploring the structure of fucosylated chondroitin sulfate through bottom-up nuclear magnetic resonance and electrospray ionization-high-resolution mass spectrometry approaches.

PubMed

Santos, Gustavo Rc; Porto, Ana Co; Soares, Paulo Ag; Vilanova, Eduardo; Mourão, Paulo As

2017-07-01

Fucosylated chondroitin sulfate (FCS) from sea cucumbers is composed of a chondroitin sulfate (CS) central core and branches of sulfated fucose. The structure of this complex glycosaminoglycan is usually investigated via nuclear magnetic resonance (NMR) analyses of the intact molecule, ergo through a top-down approach, which often yield spectra with intricate sets of signals. Here we employed a bottom-up approach to analyze the FCSs from the sea cucumbers Isostichopus badionotus and Ludwigothurea grisea from their basic constituents, viz. CS cores and sulfated fucose branches, obtained via systematic fragmentation through mild acid hydrolysis. Oligosaccharides derived from the central CS core were analyzed via NMR spectroscopy and the disaccharides produced using chondroitin sulfate lyase via SAX-HPLC. The CS cores from the two species were similar, showing only slight differences in the proportions of 4- or 6-monosulfated and 4,6-disulfated β-d-GalNAc. Sulfated fucose units released from the FCSs were analyzed via NMR and ESI-HRMS spectroscopies. The fucose units from each species presented extensive qualitative differences, but quantitative assessments of these units were hindered, mostly because of their extensive desulfation during the hydrolysis. The bottom-up analysis performed here has proved useful to explore the structure of FCS through a sum-of-the-parts approach in a qualitative manner. We further demonstrate that under specific acidification conditions particular fucose branches can be removed preferentially from FCS. Preparation of derivatives enriched with particular fucose branches could be useful for studies on "structure vs. biological function" of FCS. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Nuclear reactor spacer grid and ductless core component

DOEpatents

Christiansen, David W.; Karnesky, Richard A.

1989-01-01

The invention relates to a nuclear reactor spacer grid member for use in a liquid cooled nuclear reactor and to a ductless core component employing a plurality of these spacer grid members. The spacer grid member is of the egg-shell type and is constructed so that the walls of the cell members of the grid member are formed of a single thickness of metal to avoid tolerance problems. Within each cell member is a hydraulic spring which laterally constrains the nuclear material bearing rod which passes through each cell member against a hardstop in response to coolant flow through the cell member. This hydraulic spring is also suitable for use in a water cooled nuclear reactor. A core component constructed of, among other components, a plurality of these spacer grid members, avoids the use of a full length duct by providing spacer sleeves about the sodium tubes passing through the spacer grid members at locations between the grid members, thereby maintaining a predetermined space between adjacent grid members.

The Impact of the Nuclear Equation of State in Core Collapse Supernovae

NASA Astrophysics Data System (ADS)

Baird, M. L.; Lentz, E. J.; Hix, W. R.; Mezzacappa, A.; Messer, O. E. B.; Liebendoerfer, M.; TeraScale Supernova Initiative Collaboration

2005-12-01

One of the key ingredients to the core collapse supernova mechanism is the physics of matter at or near nuclear density. Included in simulations as part of the Equation of State (EOS), nuclear repulsion experienced at high densities are responsible for the bounce shock, which initially causes the outer envelope of the supernova to expand, as well as determining the structure of the newly formed proto-neutron star. Recent years have seen renewed interest in this fundamental piece of supernova physics, resulting in several promising candidate EOS parameterizations. We will present the impact of these variations in the nuclear EOS using spherically symmetric, Newtonian and General Relativistic neutrino transport simulations of stellar core collapse and bounce. This work is supported in part by SciDAC grants to the TeraScale Supernovae Initiative from the DOE Office of Science High Energy, Nuclear, and Advanced Scientific Computing Research Programs. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for U.S. Department of Energy under contract DEAC05-00OR22725

Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum

PubMed Central

2012-01-01

Background The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes, however, we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleus, which controls the parasite genome in a development- and cell cycle-specific manner through mostly unknown mechanisms. To study this important organelle in detail, we conducted an integrative analysis of the P. falciparum nuclear proteome. Results We combined high accuracy mass spectrometry and bioinformatic approaches to present for the first time an experimentally determined core nuclear proteome for P. falciparum. Besides a large number of factors implicated in known nuclear processes, one-third of all detected proteins carry no functional annotation, including many phylum- or genus-specific factors. Importantly, extensive experimental validation using 30 transgenic cell lines confirmed the high specificity of this inventory, and revealed distinct nuclear localization patterns of hitherto uncharacterized proteins. Further, our detailed analysis identified novel protein domains potentially implicated in gene transcription pathways, and sheds important new light on nuclear compartments and processes including regulatory complexes, the nucleolus, nuclear pores, and nuclear import pathways. Conclusion Our study provides comprehensive new insight into the biology of the Plasmodium nucleus and will serve as an important platform for dissecting general and parasite-specific nuclear processes in malaria parasites. Moreover, as the first nuclear proteome characterized in any protist organism, it will provide an important resource for studying evolutionary aspects of nuclear biology. PMID:23181666

Physics and potentials of fissioning plasmas for space power and propulsion

NASA Technical Reports Server (NTRS)

Thom, K.; Schwenk, F. C.; Schneider, R. T.

1976-01-01

Fissioning uranium plasmas are the nuclear fuel in conceptual high-temperature gaseous-core reactors for advanced rocket propulsion in space. A gaseous-core nuclear rocket would be a thermal reactor in which an enriched uranium plasma at about 10,000 K is confined in a reflector-moderator cavity where it is nuclear critical and transfers its fission power to a confining propellant flow for the production of thrust at a specific impulse up to 5000 sec. With a thrust-to-engine weight ratio approaching unity, the gaseous-core nuclear rocket could provide for propulsion capabilities needed for manned missions to the nearby planets and for economical cislunar ferry services. Fueled with enriched uranium hexafluoride and operated at temperatures lower than needed for propulsion, the gaseous-core reactor scheme also offers significant benefits in applications for space and terrestrial power. They include high-efficiency power generation at low specific mass, the burnup of certain fission products and actinides, the breeding of U-233 from thorium with short doubling times, and improved convenience of fuel handling and processing in the gaseous phase.

Adaptive multi-resolution 3D Hartree-Fock-Bogoliubov solver for nuclear structure

NASA Astrophysics Data System (ADS)

Pei, J. C.; Fann, G. I.; Harrison, R. J.; Nazarewicz, W.; Shi, Yue; Thornton, S.

2014-08-01

Background: Complex many-body systems, such as triaxial and reflection-asymmetric nuclei, weakly bound halo states, cluster configurations, nuclear fragments produced in heavy-ion fusion reactions, cold Fermi gases, and pasta phases in neutron star crust, are all characterized by large sizes and complex topologies in which many geometrical symmetries characteristic of ground-state configurations are broken. A tool of choice to study such complex forms of matter is an adaptive multi-resolution wavelet analysis. This method has generated much excitement since it provides a common framework linking many diversified methodologies across different fields, including signal processing, data compression, harmonic analysis and operator theory, fractals, and quantum field theory. Purpose: To describe complex superfluid many-fermion systems, we introduce an adaptive pseudospectral method for solving self-consistent equations of nuclear density functional theory in three dimensions, without symmetry restrictions. Methods: The numerical method is based on the multi-resolution and computational harmonic analysis techniques with a multi-wavelet basis. The application of state-of-the-art parallel programming techniques include sophisticated object-oriented templates which parse the high-level code into distributed parallel tasks with a multi-thread task queue scheduler for each multi-core node. The internode communications are asynchronous. The algorithm is variational and is capable of solving coupled complex-geometric systems of equations adaptively, with functional and boundary constraints, in a finite spatial domain of very large size, limited by existing parallel computer memory. For smooth functions, user-defined finite precision is guaranteed. Results: The new adaptive multi-resolution Hartree-Fock-Bogoliubov (HFB) solver madness-hfb is benchmarked against a two-dimensional coordinate-space solver hfb-ax that is based on the B-spline technique and a three-dimensional solver hfodd that is based on the harmonic-oscillator basis expansion. Several examples are considered, including the self-consistent HFB problem for spin-polarized trapped cold fermions and the Skyrme-Hartree-Fock (+BCS) problem for triaxial deformed nuclei. Conclusions: The new madness-hfb framework has many attractive features when applied to nuclear and atomic problems involving many-particle superfluid systems. Of particular interest are weakly bound nuclear configurations close to particle drip lines, strongly elongated and dinuclear configurations such as those present in fission and heavy-ion fusion, and exotic pasta phases that appear in neutron star crust.

Epigenetic regulation of vascular smooth muscle cell function in atherosclerosis.

PubMed

Findeisen, Hannes M; Kahles, Florian K; Bruemmer, Dennis

2013-04-01

Epigenetics involve heritable and acquired changes in gene transcription that occur independently of the DNA sequence. Epigenetic mechanisms constitute a hierarchic upper-level of transcriptional control through complex modifications of chromosomal components and nuclear structures. These modifications include, for example, DNA methylation or post-translational modifications of core histones; they are mediated by various chromatin-modifying enzymes; and ultimately they define the accessibility of a transcriptional complex to its target DNA. Integrating epigenetic mechanisms into the pathophysiologic concept of complex and multifactorial diseases such as atherosclerosis may significantly enhance our understanding of related mechanisms and provide promising therapeutic approaches. Although still in its infancy, intriguing scientific progress has begun to elucidate the role of epigenetic mechanisms in vascular biology, particularly in the control of smooth muscle cell phenotypes. In this review, we will summarize epigenetic pathways in smooth muscle cells, focusing on mechanisms involved in the regulation of vascular remodeling.

Epigenetic regulation of vascular smooth muscle cell function in atherosclerosis.

PubMed

Findeisen, Hannes M; Kahles, Florian K; Bruemmer, Dennis

2013-05-01

Epigenetics involve heritable and acquired changes in gene transcription that occur independently of the DNA sequence. Epigenetic mechanisms constitute a hierarchic upper-level of transcriptional control through complex modifications of chromosomal components and nuclear structures. These modifications include, for example, DNA methylation or post-translational modifications of core histones; they are mediated by various chromatin-modifying enzymes; and ultimately they define the accessibility of a transcriptional complex to its target DNA. Integrating epigenetic mechanisms into the pathophysiologic concept of complex and multifactorial diseases such as atherosclerosis may significantly enhance our understanding of related mechanisms and provide promising therapeutic approaches. Although still in its infancy, intriguing scientific progress has begun to elucidate the role of epigenetic mechanisms in vascular biology, particularly in the control of smooth muscle cell phenotypes. In this review, we will summarize epigenetic pathways in smooth muscle cells, focusing on mechanisms involved in the regulation of vascular remodeling.

The eukaryotic RNA exosome: same scaffold but variable catalytic subunits.

PubMed

Lykke-Andersen, Søren; Tomecki, Rafal; Jensen, Torben Heick; Dziembowski, Andrzej

2011-01-01

The RNA exosome is a versatile ribonucleolytic protein complex that participates in a multitude of cellular RNA processing and degradation events. It consists of an invariable nine-subunit core that associates with a variety of enzymatically active subunits and co-factors. These contribute to or even provide the catalytic activity and substrate specificity of the complex. The S. cerevisiae exosome has been intensively studied since its discovery in 1997 and thus serves as the archetype of eukaryotic exosomes. Notably, its catalytic potential, derived exclusively from associated subunits, differs between the nuclear and cytoplasmic versions of the complex. The same holds true for other eukaryotes, however, recent discoveries from various laboratories including our own have revealed that there are variations on this theme. Here, we review the latest findings concerning catalytic subunits of eukaryotic exosomes, and we discuss the apparent need for differential composition and subcellular distribution of exosome variants.

A Weak Bar Potential and Massive Core in the Seyfert 2 Galaxy NGC 3079: CO(1--0) observations using the Nobeyama Millimeter Array

NASA Astrophysics Data System (ADS)

Koda, J.; Sofue, Y.; Kohno, K.; Okumura, S. K.; Irwin, Judith A.

We present our recent 12CO (1-0) observations in the central molecular disk of the Hα/radio lobe galaxy NGC 3079 with the Nobeyama Millimeter Array. We show four kinematically distinct components in the observed molecular disk: a main disk, spiral arms, a nuclear disk and a nuclear core. We discuss their possible origins using a simple orbit-analysis model in a weak bar potential. We show that three of the four components are well-understood by typical gaseous orbits in a weak bar, such as gaseous x1- and x2-orbits. The main disk and spiral arms are well-understood as the gaseous x1-orbits and their associated crowding, respectively. The nuclear disk is naturally explained by the x2-orbits. However, the nuclear core, showing a high velocity of about 200kmps at a radius of about 100pc, cannot be explained by those gaseous orbits in a bar. Furthermore, no other orbits, derived by bars, cannot be responsible for the nuclear core. Thus we discuss that this component should be attributed to a central massive core with a dynamical mass of about 109Msun within the central 100pc radius. This mass is three orders of magnitude more massive than that of a central black hole in this galaxy. More detailed descriptions are presented in Koda et al. (2002).

High resolution imaging of galaxy cores

NASA Technical Reports Server (NTRS)

Crane, P.; Stiavelli, M.; King, I. R.; Deharveng, J. M.; Albrecht, R.; Barbieri, C.; Blades, J. C.; Boksenberg, A.; Disney, M. J.; Jakobsen, P.

1993-01-01

Surface photometry data obtained with the Faint Object Camera of the Hubble Space Telescope in the cores of ten galaxies is presented. The major results are: (1) none of the galaxies show truly 'isothermal' cores, (2) galaxies with nuclear activity show very similar light profiles, (3) all objects show central mass densities above 10 exp 3 solar masses/cu pc3, and (4) four of the galaxies (M87, NGC 3862, NGC 4594, NGC 6251) show evidence for exceptional nuclear mass concentrations.

Horizontal baffle for nuclear reactors

DOEpatents

Rylatt, John A.

1978-01-01

A horizontal baffle disposed in the annulus defined between the core barrel and the thermal liner of a nuclear reactor thereby physically separating the outlet region of the core from the annular area below the horizontal baffle. The horizontal baffle prevents hot coolant that has passed through the reactor core from thermally damaging apparatus located in the annulus below the horizontal baffle by utilizing the thermally induced bowing of the horizontal baffle to enhance sealing while accommodating lateral motion of the baffle base plate.

Exploratory study of several advanced nuclear-MHD power plant systems.

NASA Technical Reports Server (NTRS)

Williams, J. R.; Clement, J. D.; Rosa, R. J.; Yang, Y. Y.

1973-01-01

In order for efficient multimegawatt closed cycle nuclear-MHD systems to become practical, long-life gas cooled reactors with exit temperatures of about 2500 K or higher must be developed. Four types of nuclear reactors which have the potential of achieving this goal are the NERVA-type solid core reactor, the colloid core (rotating fluidized bed) reactor, the 'light bulb' gas core reactor, and the 'coaxial flow' gas core reactor. Research programs aimed at developing these reactors have progressed rapidly in recent years so that prototype power reactors could be operating by 1980. Three types of power plant systems which use these reactors have been analyzed to determine the operating characteristics, critical parameters and performance of these power plants. Overall thermal efficiencies as high as 80% are projected, using an MHD turbine-compressor cycle with steam bottoming, and slightly lower efficiencies are projected for an MHD motor-compressor cycle.

An improved heat transfer configuration for a solid-core nuclear thermal rocket engine

NASA Technical Reports Server (NTRS)

Clark, John S.; Walton, James T.; Mcguire, Melissa L.

1992-01-01

Interrupted flow, impingement cooling, and axial power distribution are employed to enhance the heat-transfer configuration of a solid-core nuclear thermal rocket engine. Impingement cooling is introduced to increase the local heat-transfer coefficients between the reactor material and the coolants. Increased fuel loading is used at the inlet end of the reactor to enhance heat-transfer capability where the temperature differences are the greatest. A thermal-hydraulics computer program for an unfueled NERVA reactor core is employed to analyze the proposed configuration with attention given to uniform fuel loading, number of channels through the impingement wafers, fuel-element length, mass-flow rate, and wafer gap. The impingement wafer concept (IWC) is shown to have heat-transfer characteristics that are better than those of the NERVA-derived reactor at 2500 K. The IWC concept is argued to be an effective heat-transfer configuration for solid-core nuclear thermal rocket engines.

A new equation of state for core-collapse supernovae based on realistic nuclear forces and including a full nuclear ensemble

NASA Astrophysics Data System (ADS)

Furusawa, S.; Togashi, H.; Nagakura, H.; Sumiyoshi, K.; Yamada, S.; Suzuki, H.; Takano, M.

2017-09-01

We have constructed a nuclear equation of state (EOS) that includes a full nuclear ensemble for use in core-collapse supernova simulations. It is based on the EOS for uniform nuclear matter that two of the authors derived recently, applying a variational method to realistic two- and three-body nuclear forces. We have extended the liquid drop model of heavy nuclei, utilizing the mass formula that accounts for the dependences of bulk, surface, Coulomb and shell energies on density and/or temperature. As for light nuclei, we employ a quantum-theoretical mass evaluation, which incorporates the Pauli- and self-energy shifts. In addition to realistic nuclear forces, the inclusion of in-medium effects on the full ensemble of nuclei makes the new EOS one of the most realistic EOSs, which covers a wide range of density, temperature and proton fraction that supernova simulations normally encounter. We make comparisons with the FYSS EOS, which is based on the same formulation for the nuclear ensemble but adopts the relativistic mean field theory with the TM1 parameter set for uniform nuclear matter. The new EOS is softer than the FYSS EOS around and above nuclear saturation densities. We find that neutron-rich nuclei with small mass numbers are more abundant in the new EOS than in the FYSS EOS because of the larger saturation densities and smaller symmetry energy of nuclei in the former. We apply the two EOSs to 1D supernova simulations and find that the new EOS gives lower electron fractions and higher temperatures in the collapse phase owing to the smaller symmetry energy. As a result, the inner core has smaller masses for the new EOS. It is more compact, on the other hand, due to the softness of the new EOS and bounces at higher densities. It turns out that the shock wave generated by core bounce is a bit stronger initially in the simulation with the new EOS. The ensuing outward propagations of the shock wave in the outer core are very similar in the two simulations, which may be an artifact, though, caused by the use of the same tabulated electron capture rates for heavy nuclei ignoring differences in the nuclear composition between the two EOSs in these computations.

Identification of both copy number variation-type and constant-type core elements in a large segmental duplication region of the mouse genome

PubMed Central

2013-01-01

Background Copy number variation (CNV), an important source of diversity in genomic structure, is frequently found in clusters called CNV regions (CNVRs). CNVRs are strongly associated with segmental duplications (SDs), but the composition of these complex repetitive structures remains unclear. Results We conducted self-comparative-plot analysis of all mouse chromosomes using the high-speed and large-scale-homology search algorithm SHEAP. For eight chromosomes, we identified various types of large SD as tartan-checked patterns within the self-comparative plots. A complex arrangement of diagonal split lines in the self-comparative-plots indicated the presence of large homologous repetitive sequences. We focused on one SD on chromosome 13 (SD13M), and developed SHEPHERD, a stepwise ab initio method, to extract longer repetitive elements and to characterize repetitive structures in this region. Analysis using SHEPHERD showed the existence of 60 core elements, which were expected to be the basic units that form SDs within the repetitive structure of SD13M. The demonstration that sequences homologous to the core elements (>70% homology) covered approximately 90% of the SD13M region indicated that our method can characterize the repetitive structure of SD13M effectively. Core elements were composed largely of fragmented repeats of a previously identified type, such as long interspersed nuclear elements (LINEs), together with partial genic regions. Comparative genome hybridization array analysis showed that whereas 42 core elements were components of CNVR that varied among mouse strains, 8 did not vary among strains (constant type), and the status of the others could not be determined. The CNV-type core elements contained significantly larger proportions of long terminal repeat (LTR) types of retrotransposon than the constant-type core elements, which had no CNV. The higher divergence rates observed in the CNV-type core elements than in the constant type indicate that the CNV-type core elements have a longer evolutionary history than constant-type core elements in SD13M. Conclusions Our methodology for the identification of repetitive core sequences simplifies characterization of the structures of large SDs and detailed analysis of CNV. The results of detailed structural and quantitative analyses in this study might help to elucidate the biological role of one of the SDs on chromosome 13. PMID:23834397

Sensitivity analysis of Monju using ERANOS with JENDL-4.0

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tamagno, P.; Van Rooijen, W. F. G.; Takeda, T.

2012-07-01

This paper deals with sensitivity analysis using JENDL-4.0 nuclear data applied to the Monju reactor. In 2010 the Japan Atomic Energy Agency - JAEA - released a new set of nuclear data: JENDL-4.0. This new evaluation is expected to contain improved data on actinides and covariance matrices. Covariance matrices are a key point in quantification of uncertainties due to basic nuclear data. For sensitivity analysis, the well-established ERANOS [1] code was chosen because of its integrated modules that allow users to perform a sensitivity analysis of complex reactor geometries. A JENDL-4.0 cross-section library is not available for ERANOS. Therefore amore » cross-section library had to be made from the original nuclear data set, available as ENDF formatted files. This is achieved by using the following codes: NJOY, CALENDF, MERGE and GECCO in order to create a library for the ECCO cell code (part of ERANOS). In order to make sure of the accuracy of the new ECCO library, two benchmark experiments have been analyzed: the MZA and MZB cores of the MOZART program measured at the ZEBRA facility in the UK. These were chosen due to their similarity to the Monju core. Using the JENDL-4.0 ECCO library we have analyzed the criticality of Monju during the restart in 2010. We have obtained good agreement with the measured criticality. Perturbation calculations have been performed between JENDL-3.3 and JENDL-4.0 based models. The isotopes {sup 239}Pu, {sup 238}U, {sup 241}Am and {sup 241}Pu account for a major part of observed differences. (authors)« less

Electrically Heated Testing of the Kilowatt Reactor Using Stirling Technology (KRUSTY) Experiment Using a Depleted Uranium Core

NASA Technical Reports Server (NTRS)

Briggs, Maxwell H.; Gibson, Marc A.; Sanzi, James

2017-01-01

The Kilopower project aims to develop and demonstrate scalable fission-based power technology for systems capable of delivering 110 kW of electric power with a specific power ranging from 2.5 - 6.5 Wkg. This technology could enable high power science missions or could be used to provide surface power for manned missions to the Moon or Mars. NASA has partnered with the Department of Energys National Nuclear Security Administration, Los Alamos National Labs, and Y-12 National Security Complex to develop and test a prototypic reactor and power system using existing facilities and infrastructure. This technology demonstration, referred to as the Kilowatt Reactor Using Stirling TechnologY (KRUSTY), will undergo nuclear ground testing in the summer of 2017 at the Nevada Test Site. The 1 kWe variation of the Kilopower system was chosen for the KRUSTY demonstration. The concept for the 1 kWe flight system consist of a 4 kWt highly enriched Uranium-Molybdenum reactor operating at 800 degrees Celsius coupled to sodium heat pipes. The heat pipes deliver heat to the hot ends of eight 125 W Stirling convertors producing a net electrical output of 1 kW. Waste heat is rejected using titanium-water heat pipes coupled to carbon composite radiator panels. The KRUSTY test, based on this design, uses a prototypic highly enriched uranium-molybdenum core coupled to prototypic sodium heat pipes. The heat pipes transfer heat to two Advanced Stirling Convertors (ASC-E2s) and six thermal simulators, which simulate the thermal draw of full scale power conversion units. Thermal simulators and Stirling engines are gas cooled. The most recent project milestone was the completion of non-nuclear system level testing using an electrically heated depleted uranium (non-fissioning) reactor core simulator. System level testing at the Glenn Research Center (GRC) has validated performance predictions and has demonstrated system level operation and control in a test configuration that replicates the one to be used at the Device Assembly Facility (DAF) at the Nevada National Security Site. Fabrication, assembly, and testing of the depleted uranium core has allowed for higher fidelity system level testing at GRC, and has validated the fabrication methods to be used on the highly enriched uranium core that will supply heat for the DAF KRUSTY demonstration.

Reactor moderator, pressure vessel, and heat rejection system of an open-cycle gas core nuclear rocket concept

NASA Technical Reports Server (NTRS)

Taylor, M. F.; Whitmarsh, C. L., Jr.; Sirocky, P. J., Jr.; Iwanczyke, L. C.

1973-01-01

A preliminary design study of a conceptual 6000-megawatt open-cycle gas-core nuclear rocket engine system was made. The engine has a thrust of 196,600 newtons (44,200 lb) and a specific impulse of 4400 seconds. The nuclear fuel is uranium-235 and the propellant is hydrogen. Critical fuel mass was calculated for several reactor configurations. Major components of the reactor (reflector, pressure vessel, and waste heat rejection system) were considered conceptually and were sized.

Nup153 and Nup98 bind the HIV-1 core and contribute to the early steps of HIV-1 replication

DOE Office of Scientific and Technical Information (OSTI.GOV)

Di Nunzio, Francesca, E-mail: francesca.di-nunzio@pasteur.fr; Fricke, Thomas; Miccio, Annarita

The early steps of HIV-1 replication involve the entry of HIV-1 into the nucleus, which is characterized by viral interactions with nuclear pore components. HIV-1 developed an evolutionary strategy to usurp the nuclear pore machinery and chromatin in order to integrate and efficiently express viral genes. In the current work, we studied the role of nucleoporins 153 and 98 (Nup153 and Nup98) in infection of human Jurkat lymphocytes by HIV-1. We showed that Nup153-depleted cells exhibited a defect in nuclear import, while depletion of Nup 98 caused a slight defect in HIV integration. To explore the biochemical viral determinants formore » the requirement of Nup153 and Nup98 during HIV-1 infection, we tested the ability of these nucleoporins to interact with HIV-1 cores. Our findings showed that both nucleoporins bind HIV-1 cores suggesting that this interaction is important for HIV-1 nuclear import and/or integration. Distribution analysis of integration sites in Nup153-depleted cells revealed a reduced tendency of HIV-1 to integrate in intragenic sites, which in part could account for the large infectivity defect observed in Nup153-depleted cells. Our work strongly supports a role for Nup153 in HIV-1 nuclear import and integration. - Highlights: ► We studied the role of Nup98 and Nup153 in HIV-1 infection. ► Nup98 binds the HIV-1 core and is involved in HIV-1 integration. ► Nup153 binds the HIV-1 core and is involved in HIV-1 nuclear import. ► Depletion of Nup153 decreased the integration of HIV-1 in transcriptionally active sites.« less

On M31's Double Nucleus

NASA Technical Reports Server (NTRS)

Miller, R. H.; Smith, B. F.; Cuzzi, Jeffrey (Technical Monitor)

1995-01-01

The recent HST discovery of a double nucleus in M31 brings into prominence the question how long, a second core can survive within the nuclear regions of a galaxy. Physical conditions in the nuclear regions of a typical galaxy help a second core survive, so it can orbit for a long time. possibly for thousands of orbits. Given the nearly uniform mass density in a core, tidal forces within a core radius are compressive in all directions and help the core survive the buffeting it takes as it orbits near the center of the galaxy. We use numerical experiments to illustrate these physical principles. Our method allows the full power of the experiments to be concentrated on the nuclear regions. Spatial resolution of about 0.2 pc comfortably resolves detail within the 1.4 parsec core radius of the second, but brighter core (P1) in M31. We use these physical principles to discuss M31's double nucleus, but they apply to other galaxies as well. and in other astronomical situations such as dumbbell galaxies. galaxies orbiting near the center of a galaxy cluster, and subclustering in galaxy clusters. The experiments also illustrate that galaxy encounters and merging are quite sensitive to external tidal forces, such as those produced by the gravitational potential in a group or cluster of galaxies.

Structural insights into the histone H1-nucleosome complex

PubMed Central

Zhou, Bing-Rui; Feng, Hanqiao; Kato, Hidenori; Dai, Liang; Yang, Yuedong; Zhou, Yaoqi; Bai, Yawen

2013-01-01

Linker H1 histones facilitate formation of higher-order chromatin structures and play important roles in various cell functions. Despite several decades of effort, the structural basis of how H1 interacts with the nucleosome remains elusive. Here, we investigated Drosophila H1 in complex with the nucleosome, using solution nuclear magnetic resonance spectroscopy and other biophysical methods. We found that the globular domain of H1 bridges the nucleosome core and one 10-base pair linker DNA asymmetrically, with its α3 helix facing the nucleosomal DNA near the dyad axis. Two short regions in the C-terminal tail of H1 and the C-terminal tail of one of the two H2A histones are also involved in the formation of the H1–nucleosome complex. Our results lead to a residue-specific structural model for the globular domain of the Drosophila H1 in complex with the nucleosome, which is different from all previous experiment-based models and has implications for chromatin dynamics in vivo. PMID:24218562

Uranium droplet core nuclear rocket

NASA Technical Reports Server (NTRS)

Anghaie, Samim

1991-01-01

Uranium droplet nuclear rocket is conceptually designed to utilize the broad temperature range ofthe liquid phase of metallic uranium in droplet configuration which maximizes the energy transfer area per unit fuel volume. In a baseline system dissociated hydrogen at 100 bar is heated to 6000 K, providing 2000 second of Isp. Fission fragments and intense radian field enhance the dissociation of molecular hydrogen beyond the equilibrium thermodynamic level. Uranium droplets in the core are confined and separated by an axisymmetric vortex flow generated by high velocity tangential injection of hydrogen in the mid-core regions. Droplet uranium flow to the core is controlled and adjusted by a twin flow nozzle injection system.

Fast-acting nuclear reactor control device

DOEpatents

Kotlyar, Oleg M.; West, Phillip B.

1993-01-01

A fast-acting nuclear reactor control device for moving and positioning a fety control rod to desired positions within the core of the reactor between a run position in which the safety control rod is outside the reactor core, and a shutdown position in which the rod is fully inserted in the reactor core. The device employs a hydraulic pump/motor, an electric gear motor, and solenoid valve to drive the safety control rod into the reactor core through the entire stroke of the safety control rod. An overrunning clutch allows the safety control rod to freely travel toward a safe position in the event of a partial drive system failure.

MODERATOR ELEMENTS FOR UNIFORM POWER NUCLEAR REACTOR

DOEpatents

Balent, R.

1963-03-12

This patent describes a method of obtaining a flatter flux and more uniform power generation across the core of a nuclear reactor. The method comprises using moderator elements having differing moderating strength. The elements have an increasing amount of the better moderating material as a function of radial and/or axial distance from the reactor core center. (AEC)

Reactivity control assembly for nuclear reactor. [LMFBR

DOEpatents

Bollinger, L.R.

1982-03-17

This invention, which resulted from a contact with the United States Department of Energy, relates to a control mechanism for a nuclear reactor and, more particularly, to an assembly for selectively shifting different numbers of reactivity modifying rods into and out of the core of a nuclear reactor. It has been proposed heretofore to control the reactivity of a breeder reactor by varying the depth of insertion of control rods (e.g., rods containing a fertile material such as ThO/sub 2/) in the core of the reactor, thereby varying the amount of neutron-thermalizing coolant and the amount of neutron-capturing material in the core. This invention relates to a mechanism which can advantageously be used in this type of reactor control system.

Comparative assessment of out-of-core nuclear thermionic power systems

NASA Technical Reports Server (NTRS)

Estabrook, W. C.; Koenig, D. R.; Prickett, W. Z.

1975-01-01

The hardware selections available for fabrication of a nuclear electric propulsion stage for planetary exploration were explored. The investigation was centered around a heat-pipe-cooled, fast-spectrum nuclear reactor for an out-of-core power conversion system with sufficient detail for comparison with the in-core system studies completed previously. A survey of competing power conversion systems still indicated that the modular reliability of thermionic converters makes them the desirable choice to provide the 240-kWe end-of-life power for at least 20,000 full power hours. The electrical energy will be used to operate a number of mercury ion bombardment thrusters with a specific impulse in the range of about 4,000-5,000 seconds.

A New Capability for Nuclear Thermal Propulsion Design

DOE Office of Scientific and Technical Information (OSTI.GOV)

Amiri, Benjamin W.; Nuclear and Radiological Engineering Department, University of Florida, Gainesville, FL 32611; Kapernick, Richard J.

2007-01-30

This paper describes a new capability for Nuclear Thermal Propulsion (NTP) design that has been developed, and presents the results of some analyses performed with this design tool. The purpose of the tool is to design to specified mission and material limits, while maximizing system thrust to weight. The head end of the design tool utilizes the ROCket Engine Transient Simulation (ROCETS) code to generate a system design and system design requirements as inputs to the core analysis. ROCETS is a modular system level code which has been used extensively in the liquid rocket engine industry for many years. Themore » core design tool performs high-fidelity reactor core nuclear and thermal-hydraulic design analysis. At the heart of this process are two codes TMSS-NTP and NTPgen, which together greatly automate the analysis, providing the capability to rapidly produce designs that meet all specified requirements while minimizing mass. A PERL based command script, called CORE DESIGNER controls the execution of these two codes, and checks for convergence throughout the process. TMSS-NTP is executed first, to produce a suite of core designs that meet the specified reactor core mechanical, thermal-hydraulic and structural requirements. The suite of designs consists of a set of core layouts and, for each core layout specific designs that span a range of core fuel volumes. NTPgen generates MCNPX models for each of the core designs from TMSS-NTP. Iterative analyses are performed in NTPgen until a reactor design (fuel volume) is identified for each core layout that meets cold and hot operation reactivity requirements and that is zoned to meet a radial core power distribution requirement.« less

Unified ab initio approaches to nuclear structure and reactions

DOE PAGES

Navratil, Petr; Quaglioni, Sofia; Hupin, Guillaume; ...

2016-04-13

The description of nuclei starting from the constituent nucleons and the realistic interactions among them has been a long-standing goal in nuclear physics. In addition to the complex nature of the nuclear forces, with two-, three- and possibly higher many-nucleon components, one faces the quantum-mechanical many-nucleon problem governed by an interplay between bound and continuum states. In recent years, significant progress has been made in ab initio nuclear structure and reaction calculations based on input from QCD-employing Hamiltonians constructed within chiral effective field theory. After a brief overview of the field, we focus on ab initio many-body approaches—built upon the no-core shell model—that are capable of simultaneously describing both bound and scattering nuclear states, and present results for resonances in light nuclei, reactions important for astrophysics and fusion research. In particular, we review recent calculations of resonances in the 6He halo nucleus, of five- and six-nucleon scattering, and an investigation of the role of chiral three-nucleon interactions in the structure of 9Be. Further, we discuss applications to the 7Bemore » $${({\\rm{p}},\\gamma )}^{8}{\\rm{B}}$$ radiative capture. Lastly, we highlight our efforts to describe transfer reactions including the 3H$${({\\rm{d}},{\\rm{n}})}^{4}$$He fusion.« less

The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families.

PubMed

Tuppen, Helen A L; Hogan, Vanessa E; He, Langping; Blakely, Emma L; Worgan, Lisa; Al-Dosary, Mazhor; Saretzki, Gabriele; Alston, Charlotte L; Morris, Andrew A; Clarke, Michael; Jones, Simon; Devlin, Anita M; Mansour, Sahar; Chrzanowska-Lightowlers, Zofia M A; Thorburn, David R; McFarland, Robert; Taylor, Robert W

2010-10-01

Isolated complex I deficiency is the most frequently observed oxidative phosphorylation defect in children with mitochondrial disease, leading to a diverse range of clinical presentations, including Leigh syndrome. For most patients the genetic cause of the biochemical defect remains unknown due to incomplete understanding of the complex I assembly process. Nonetheless, a plethora of pathogenic mutations have been described to date in the seven mitochondrial-encoded subunits of complex I as well as in 12 of the nuclear-encoded subunits and in six assembly factors. Whilst several mitochondrial DNA mutations are recurrent, the majority of these mutations are reported in single families. We have sequenced core structural and functional nuclear-encoded subunits of complex I in a cohort of 34 paediatric patients with isolated complex I deficiency, identifying pathogenic mutations in 6 patients. These included a novel homozygous NDUFS1 mutation in an Asian child with Leigh syndrome, a previously identified NDUFS8 mutation (c.236C>T, p.P79L) in a second Asian child with Leigh-like syndrome and six novel, compound heterozygous NDUFS2 mutations in four white Caucasian patients with Leigh or Leigh-like syndrome. Three of these children harboured an identical NDUFS2 mutation (c.875T>C, p.M292T), which was also identified in conjunction with a novel NDUFS2 splice site mutation (c.866+4A>G) in a fourth Caucasian child who presented to a different diagnostic centre, with microsatellite and single nucleotide polymorphism analyses indicating that this was due to an ancient common founder event. Our results confirm that NDUFS2 is a mutational hotspot in Caucasian children with isolated complex I deficiency and recommend the routine diagnostic investigation of this gene in patients with Leigh or Leigh-like phenotypes.

Application of a Virtual Reactivity Feedback Control Loop in Non-Nuclear Testing of a Fast Spectrum Reactor

NASA Technical Reports Server (NTRS)

Bragg-Sitton, Shannon M.; Forsbacka, Matthew

2004-01-01

For a compact, fast-spectrum reactor, reactivity feedback is dominated by core deformation at elevated temperature. Given the use of accurate deformation measurement techniques, it is possible to simulate nuclear feedback in non-nuclear electrically heated reactor tests. Implementation of simulated reactivity feedback in response to measured deflection is being tested at the NASA Marshall Space Flight Center Early Flight Fission Test Facility (EFF-TF). During tests of the SAFE-100 reactor prototype, core deflection was monitored using a high resolution camera. "virtual" reactivity feedback was accomplished by applying the results of Monte Carlo calculations (MCNPX) to core deflection measurements; the computational analysis was used to establish the reactivity worth of van'ous core deformations. The power delivered to the SAFE-100 prototype was then dusted accordingly via kinetics calculations, The work presented in this paper will demonstrate virtual reactivity feedback as core power was increased from 1 kilowatt(sub t), to 10 kilowatts(sub t), held approximately constant at 10 kilowatts (sub t), and then allowed to decrease based on the negative thermal reactivity coefficient.

An underground nuclear power station using self-regulating heat-pipe controlled reactors

DOEpatents

Hampel, V.E.

1988-05-17

A nuclear reactor for generating electricity is disposed underground at the bottom of a vertical hole that can be drilled using conventional drilling technology. The primary coolant of the reactor core is the working fluid in a plurality of thermodynamically coupled heat pipes emplaced in the hole between the heat source at the bottom of the hole and heat exchange means near the surface of the earth. Additionally, the primary coolant (consisting of the working fluid in the heat pipes in the reactor core) moderates neutrons and regulates their reactivity, thus keeping the power of the reactor substantially constant. At the end of its useful life, the reactor core may be abandoned in place. Isolation from the atmosphere in case of accident or for abandonment is provided by the operation of explosive closures and mechanical valves emplaced along the hole. This invention combines technology developed and tested for small, highly efficient, space-based nuclear electric power plants with the technology of fast- acting closure mechanisms developed and used for underground testing of nuclear weapons. This invention provides a nuclear power installation which is safe from the worst conceivable reactor accident, namely, the explosion of a nuclear weapon near the ground surface of a nuclear power reactor. 5 figs.

Underground nuclear power station using self-regulating heat-pipe controlled reactors

DOEpatents

Hampel, Viktor E.

1989-01-01

A nuclear reactor for generating electricity is disposed underground at the bottom of a vertical hole that can be drilled using conventional drilling technology. The primary coolant of the reactor core is the working fluid in a plurality of thermodynamically coupled heat pipes emplaced in the hole between the heat source at the bottom of the hole and heat exchange means near the surface of the earth. Additionally, the primary coolant (consisting of the working flud in the heat pipes in the reactor core) moderates neutrons and regulates their reactivity, thus keeping the power of the reactor substantially constant. At the end of its useful life, the reactor core may be abandoned in place. Isolation from the atmosphere in case of accident or for abandonment is provided by the operation of explosive closures and mechanical valves emplaced along the hole. This invention combines technology developed and tested for small, highly efficient, space-based nuclear electric power plants with the technology of fast-acting closure mechanisms developed and used for underground testing of nuclear weapons. This invention provides a nuclear power installation which is safe from the worst conceivable reactor accident, namely, the explosion of a nuclear weapon near the ground surface of a nuclear power reactor.

Fabrication and Testing of a Modular Micro-Pocket Fission Detector Instrumentation System for Test Nuclear Reactors

NASA Astrophysics Data System (ADS)

Reichenberger, Michael A.; Nichols, Daniel M.; Stevenson, Sarah R.; Swope, Tanner M.; Hilger, Caden W.; Roberts, Jeremy A.; Unruh, Troy C.; McGregor, Douglas S.

2018-01-01

Advancements in nuclear reactor core modeling and computational capability have encouraged further development of in-core neutron sensors. Measurement of the neutron-flux distribution within the reactor core provides a more complete understanding of the operating conditions in the reactor than typical ex-core sensors. Micro-Pocket Fission Detectors have been developed and tested previously but have been limited to single-node operation and have utilized highly specialized designs. The development of a widely deployable, multi-node Micro-Pocket Fission Detector assembly will enhance nuclear research capabilities. A modular, four-node Micro-Pocket Fission Detector array was designed, fabricated, and tested at Kansas State University. The array was constructed from materials that do not significantly perturb the neutron flux in the reactor core. All four sensor nodes were equally spaced axially in the array to span the fuel-region of the reactor core. The array was filled with neon gas, serving as an ionization medium in the small cavities of the Micro-Pocket Fission Detectors. The modular design of the instrument facilitates the testing and deployment of numerous sensor arrays. The unified design drastically improved device ruggedness and simplified construction from previous designs. Five 8-mm penetrations in the upper grid plate of the Kansas State University TRIGA Mk. II research nuclear reactor were utilized to deploy the array between fuel elements in the core. The Micro-Pocket Fission Detector array was coupled to an electronic support system which has been specially developed to support pulse-mode operation. The Micro-Pocket Fission Detector array composed of four sensors was used to monitor local neutron flux at a constant reactor power of 100 kWth at different axial locations simultaneously. The array was positioned at five different radial locations within the core to emulate the deployment of multiple arrays and develop a 2-dimensional measurement of neutron flux in the reactor core.

Numerical studies of nonspherical carbon combustion models

NASA Astrophysics Data System (ADS)

Mueller, E.; Arnett, W. D.

1982-10-01

First results of axisymmetric numerical studies of the final evolution of degenerate C + O cores are reported. The two-dimensional convective flow is treated without a phenomenological theory of convection. The computations show that, in the beginning, the nuclear burning propagates slowly outward from the center of the star in a spherical combustion front. Small-scale eddies form, giving rise to bumps in the front. The bumps grow into blobs and eventually into fingers, which steadily elongate relative to the rest of the combustion front. This behavior is not well described by either the detonation or deflagration models, being more complex than either.

Core Histones and HIRIP3, a Novel Histone-Binding Protein, Directly Interact with WD Repeat Protein HIRA

PubMed Central

Lorain, Stéphanie; Quivy, Jean-Pierre; Monier-Gavelle, Frédérique; Scamps, Christine; Lécluse, Yann; Almouzni, Geneviève; Lipinski, Marc

1998-01-01

The human HIRA gene has been named after Hir1p and Hir2p, two corepressors which together appear to act on chromatin structure to control gene transcription in Saccharomyces cerevisiae. HIRA homologs are expressed in a regulated fashion during mouse and chicken embryogenesis, and the human gene is a major candidate for the DiGeorge syndrome and related developmental disorders caused by a reduction to single dose of a fragment of chromosome 22q. Western blot analysis and double-immunofluorescence experiments using a specific antiserum revealed a primary nuclear localization of HIRA. Similar to Hir1p, HIRA contains seven amino-terminal WD repeats and probably functions as part of a multiprotein complex. HIRA and core histone H2B were found to physically interact in a yeast double-hybrid protein interaction trap, in GST pull-down assays, and in coimmunoprecipitation experiments performed from cellular extracts. In vitro, HIRA also interacted with core histone H4. H2B- and H4-binding domains were overlapping but distinguishable in the carboxy-terminal region of HIRA, and the region for HIRA interaction was mapped to the amino-terminal tail of H2B and the second α helix of H4. HIRIP3 (HIRA-interacting protein 3) is a novel gene product that was identified from its HIRA-binding properties in the yeast protein interaction trap. In vitro, HIRIP3 directly interacted with HIRA but also with core histones H2B and H3, suggesting that a HIRA-HIRIP3-containing complex could function in some aspects of chromatin and histone metabolism. Insufficient production of HIRA, which we report elsewhere interacts with homeodomain-containing DNA-binding factors during mammalian embryogenesis, could perturb the stoichiometric assembly of multimolecular complexes required for normal embryonic development. PMID:9710638

Apparatus for controlling nuclear core debris

DOEpatents

Jones, Robert D.

1978-01-01

Nuclear reactor apparatus for containing, cooling, and dispersing reactor debris assumed to flow from the core area in the unlikely event of an accident causing core meltdown. The apparatus includes a plurality of horizontally disposed vertically spaced plates, having depressions to contain debris in controlled amounts, and a plurality of holes therein which provide natural circulation cooling and a path for debris to continue flowing downward to the plate beneath. The uppermost plates may also include generally vertical sections which form annular-like flow areas which assist the natural circulation cooling.

Gfr Core Neutronics Studies at CEA

NASA Astrophysics Data System (ADS)

Bosq, J. C.; Brun-Magaud, V.; Rimpault, G.; Tommasi, J.; Conti, A.; Garnier, J. C.

2006-04-01

The Gas cooled Fast Reactor (GFR) is a high priority in the CEA R&D program on Future Nuclear Energy Systems. After preliminary neutronics and thermo-aerolic studies, a first He-cooled 2400MWth core design based on a series of carbide CERCER plates arranged in an hexagonal wrapper were selected. Although GFR subassembly and core design studies are still at an early stage of development, it is nonetheless possible to identify a number of nuclear data needs that could have some impact on the actual design: new materials, decay heat contributors….

Three-dimensional pin-to-pin analyses of VVER-440 cores by the MOBY-DICK code

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lehmann, M.; Mikolas, P.

1994-12-31

Nuclear design for the Dukovany (EDU) VVER-440s nuclear power plant is routinely performed by the MOBY-DICK system. After its implementation on Hewlett Packard series 700 workstations, it is able to perform routinely three-dimensional pin-to-pin core analyses. For purposes of code validation, the benchmark prepared from EDU operational data was solved.

Packed rod neutron shield for fast nuclear reactors

DOEpatents

Eck, John E.; Kasberg, Alvin H.

1978-01-01

A fast neutron nuclear reactor including a core and a plurality of vertically oriented neutron shield assemblies surrounding the core. Each assembly includes closely packed cylindrical rods within a polygonal metallic duct. The shield assemblies are less susceptible to thermal stresses and are less massive than solid shield assemblies, and are cooled by liquid coolant flow through interstices among the rods and duct.

GAS COOLED NUCLEAR REACTORS

DOEpatents

Long, E.; Rodwell, W.

1958-06-10

A gas-cooled nuclear reactor consisting of a graphite reacting core and reflector structure supported in a containing vessel is described. A gas sealing means is included for sealing between the walls of the graphite structure and containing vessel to prevent the gas coolant by-passing the reacting core. The reacting core is a multi-sided right prismatic structure having a pair of parallel slots around its periphery. The containing vessel is cylindrical and has a rib on its internal surface which supports two continuous ring shaped flexible web members with their radially innermost ends in sealing engagement within the radially outermost portion of the slots. The core structure is supported on ball bearings. This design permits thermal expansion of the core stracture and vessel while maintainirg a peripheral seal between the tvo elements.

The TOC complex: preprotein gateway to the chloroplast.

PubMed

Andrès, Charles; Agne, Birgit; Kessler, Felix

2010-06-01

Photosynthetic eukaryotes strongly depend on chloroplast metabolic pathways. Most if not all involve nuclear encoded proteins. These are synthesized as cytosolic preproteins with N-terminal, cleavable targeting sequences (transit peptide). Preproteins are imported by a major pathway composed of two proteins complexes: TOC and TIC (Translocon of the Outer and Inner membranes of the Chloroplasts, respectively). These selectively recognize the preproteins and facilitate their transport across the chloroplast envelope. The TOC core complex consists of three types of components, each belonging to a small family: Toc34, Toc75 and Toc159. Toc34 and Toc159 isoforms represent a subfamily of the GTPase superfamily. The members of the Toc34 and Toc159 subfamily act as GTP-dependent receptors at the chloroplast surface and distinct members of each occur in defined, substrate-specific TOC complexes. Toc75, a member of the Omp85 family, is conserved from prokaryotes and functions as the unique protein-conducting channel at the outer membrane. In this review we will describe the current state of knowledge regarding the composition and function of the TOC complex.

Parameter study of dual-mode space nuclear fission solid core power and propulsion systems, NUROC3A. AMS report No. 1239c

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, W.W.; Layton, J.P.

1976-09-13

The three-volume report describes a dual-mode nuclear space power and propulsion system concept that employs an advanced solid-core nuclear fission reactor coupled via heat pipes to one of several electric power conversion systems. The NUROC3A systems analysis code was designed to provide the user with performance characteristics of the dual-mode system. Volume 3 describes utilization of the NUROC3A code to produce a detailed parameter study of the system.

Water-stable fac-{TcO₃}⁺ complexes - a new field of technetium chemistry.

PubMed

Braband, Henrik

2011-01-01

The development of technetium chemistry has been lagging behind that of its heavier congener rhenium, primarily because the inherent radioactivity of all Tc isotopes has limited the number of laboratories that can study the chemistry of this fascinating element. Although technetium is an artificial element, it is not rare. Significant amounts of the isotope (99)Tc are produced every day as a fission byproduct in nuclear power plants. Therefore, a fundamental understanding of the chemistry of (99)Tc is essential to avoid its release into the environment. In this article the chemistry of technetium at its highest oxidation state (+VII) is reviewed with a special focus on recent developments which make water-stable complexes of the general type [TcO(3)(tacn-R)](+) (tacn-R = 1,4,7-triazacyclononane or derivatives) accessible. Complexes containing the fac-{TcO(3)}(+) core display a unique reactivity. In analogy to [OsO(4)] and [RuO(4)], complexes containing the fac-{TcO(3)}(+) core undergo with alkenes metal-mediated, vicinal cis-dihydroxylation reactions (alkene-glycol interconversion) in water via a (3+2)-cycloaddition reaction. Therefore, water-stable fac-{(99m)TcO(3)}(+) complexes pave the way for a new labeling strategy for radiopharmaceutical applications, based on (3+2)-cycloaddition reactions. This new concept for the labeling of biomolecules with small [(99m)TcO(3)(tacn-R)](+)-type complexes by way of a (3+2)-cycloaddition with alkenes is discussed in detail. The herein reported developments in high-valent technetium chemistry create a new field of research with this artificial element. This demonstrates the potential of fundamental research to provide new impetus of innovation for the development of new methods for radiopharmaceutical applications.

Dual annular rotating "windowed" nuclear reflector reactor control system

DOEpatents

Jacox, Michael G.; Drexler, Robert L.; Hunt, Robert N. M.; Lake, James A.

1994-01-01

A nuclear reactor control system is provided in a nuclear reactor having a core operating in the fast neutron energy spectrum where criticality control is achieved by neutron leakage. The control system includes dual annular, rotatable reflector rings. There are two reflector rings: an inner reflector ring and an outer reflector ring. The reflectors are concentrically assembled, surround the reactor core, and each reflector ring includes a plurality of openings. The openings in each ring are capable of being aligned or non-aligned with each other. Independent driving means for each of the annular reflector rings is provided so that reactor criticality can be initiated and controlled by rotation of either reflector ring such that the extent of alignment of the openings in each ring controls the reflection of neutrons from the core.

77 FR 36014 - Initial Test Program of Emergency Core Cooling Systems for Boiling-Water Reactors

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... NUCLEAR REGULATORY COMMISSION [NRC-2012-0134] Initial Test Program of Emergency Core Cooling... for public comment draft regulatory guide (DG), DG-1277, ``Initial Test Program of Emergency Core... acceptable to implement with regard to initial testing features of emergency core cooling systems (ECCSs) for...

Polynuclear Hydroxido-Bridged Complexes of Platinum(IV) with Terminal Nitrato Ligands.

PubMed

Vasilchenko, Danila; Berdugin, Semen; Tkachev, Sergey; Baidina, Iraida; Romanenko, Galina; Gerasko, Olga; Korenev, Sergey

2015-05-18

For the first time the polynuclear hydroxido-bridged platinum(IV) nitrato complexes with nuclearity higher than two were isolated from nitric acid solutions of [Pt(H2O)2(OH)4] and crystallized as supramolecular compounds of macrocyclic cavitands cucurbit[n]uril (CB[n], n = 6,8) and 18-crown-6 ether: [Pt4(μ3-OH)2(μ2-OH)4(NO3)10]·CB[6]·25H2O (I), [Pt6(μ3-OH)4(μ2-OH)6(NO3)12](NO3)2·CB[8]·50H2O (II), and [H3O⊂18-crown-6]2[Pt2(μ2-OH)2(NO3)8][Pt4(μ3-OH)2(μ2-OH)4(NO3)10] (III). The isolation of the compounds in the single crystalline state allows the determination of the structure of the tetranuclear and hexanuclear complexes [Pt4(μ3-OH)2(μ2-OH)4(NO3)10] and [Pt6(μ3-OH)4(μ2-OH)6(NO3)12](2+), which have been previously unknown in the solid state. Stability of Ptx(OH)y cores of the polynuclear nitrato complexes toward alkaline hydrolysis was verified by (195)Pt NMR spectroscopy. Analysis of (195)Pt NMR spectra of the compound III reveals that addition of every Pt(μ-OH)2Pt ring results in ∼260 ppm downfield shift relative to the mononuclear form, which allows the prediction of signal positions for complexes of higher nuclearity.

Reconstructed plutonium fallout in the GV7 firn core from Northern Victoria Land, East Antarctica

NASA Astrophysics Data System (ADS)

Hwang, H.; Han, Y.; Kang, J.; Lee, K.; Hong, S.; Hur, S. D.; Narcisi, B.; Frezzotti, M.

2017-12-01

Atmospheric nuclear explosions during the period from the 1940s to the 1980s are the major anthropogenic source of plutonium (Pu) in the environment. In this work, we analyzed fg g-1 levels of artificial Pu, released predominantly by atmospheric nuclear weapons tests. We measured 351 samples which collected a 78 m-depth fire core at the site of GV7 (S 70°41'17.1", E 158°51'48.9", 1950 m a.s.l.), Northern Victoria Land, East Antarctica. To determine the Pu concentration in the samples, we used an inductively coupled plasma sector field mass spectrometry coupled with an Apex high-efficiency sample introduction system, which has the advantages of small sample consumption and simple sample preparation. We reconstructed the firn core Pu fallout record for the period after 1954 CE shows a significant fluctuation in agreement with past atmospheric nuclear testing. These data will contribute to ice core research by providing depth-age information.

Analysis of the return to power scenario following a LBLOCA in a PWR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Macian, R.; Tyler, T.N.; Mahaffy, J.H.

1995-09-01

The risk of reactivity accidents has been considered an important safety issue since the beginning of the nuclear power industry. In particular, several events leading to such scenarios for PWR`s have been recognized and studied to assess the potential risk of fuel damage. The present paper analyzes one such event: the possible return to power during the reflooding phase following a LBLOCA. TRAC-PF1/MOD2 coupled with a three-dimensional neutronic model of the core based on the Nodal Expansion Method (NEM) was used to perform the analysis. The system computer model contains a detailed representation of a complete typical 4-loop PWR. Thus,more » the simulation can follow complex system interactions during reflooding, which may influence the neutronics feedback in the core. Analyses were made with core models bases on cross sections generated by LEOPARD. A standard and a potentially more limiting case, with increased pressurizer and accumulator inventories, were run. In both simulations, the reactor reaches a stable state after the reflooding is completed. The lower core region, filled with cold water, generates enough power to boil part of the incoming liquid, thus preventing the core average liquid fraction from reaching a value high enough to cause a return to power. At the same time, the mass flow rate through the core is adequate to maintain the rod temperature well below the fuel damage limit.« less

Nuclear quantum effects on structure and transport properties of dense liquid helium

NASA Astrophysics Data System (ADS)

Kang, Dongdong; Dai, Jiayu; Yuan, Jianmin

2015-11-01

Transport properties of dense liquid helium under the conditions of planet's core and cool atmosphere of white dwarfs are important for determining the structure and evolution of these astrophysical objects. We have investigated these properties of dense liquid helium by using the improved centroid path-integral simulations combined with density functional theory. The results show that with the inclusion of nuclear quantum effects (NQEs), the self-diffusion is largely higher while the shear viscosity is notably lower than the results of without the inclusion of NQEs due to the lower collision cross sections even when the NQEs have little effects on the static structures. The potential surface of helium atom along the simulation trajectory is quite different between MD and PIMD simulations. We have shown that the quantum nuclear character induces complex behaviors for ionic transport properties of dense liquid helium. NQEs bring more fluctuations of local electronic density of states than the classical treatment. Therefore, in order to construct more reasonable structure and evolution model for the planets and WDs, NQEs must be reconsidered when calculating the transport properties at certain temperature and density conditions.

A prototype experiment for cooperative monitoring of nuclear reactors with cubic meter scale antineutrino detectors

NASA Astrophysics Data System (ADS)

Bernstein, A.; Allen, M.; Bowden, N.; Brennan, J.; Carr, D. J.; Estrada, J.; Hagmann, C.; Lund, J. C.; Madden, N. W.; Winant, C. D.

2005-09-01

Our Lawrence Livermore National Laboratory/Sandia National Laboratories collaboration has deployed a cubic-meter-scale antineutrino detector to demonstrate non-intrusive and automatic monitoring of the power levels and plutonium content of a nuclear reactor. Reactor monitoring of this kind is required for all non-nuclear weapons states under the Nuclear Nonproliferation Treaty (NPT), and is implemented by the International Atomic Energy Agency (IAEA). Since the antineutrino count rate and energy spectrum depend on the relative yields of fissioning isotopes in the reactor core, changes in isotopic composition can be observed without ever directly accessing the core. Data from a cubic meter scale antineutrino detector, coupled with the well-understood principles that govern the core's evolution in time, can be used to determine whether the reactor is being operated in an illegitimate way. Our group has deployed a detector at the San Onofre reactor site in California to demonstrate this concept. This paper describes the concept and shows preliminary results from 8 months of operation.

The host galaxy/AGN connection in nearby early-type galaxies. Is there a miniature radio-galaxy in every "core" galaxy?

NASA Astrophysics Data System (ADS)

Balmaverde, B.; Capetti, A.

2006-02-01

This is the second of a series of three papers exploring the connection between the multiwavelength properties of AGN in nearby early-type galaxies and the characteristics of their hosts. We selected two samples with 5 GHz VLA radio flux measurements down to 1 mJy, reaching levels of radio luminosity as low as 1036 erg s-1. In Paper I we presented a study of the surface brightness profiles for the 65 objects with available archival HST images out of the 116 radio-detected galaxies. We classified early-type galaxies into "core" and "power-law" galaxies, discriminating on the basis of the slope of their nuclear brightness profiles, following the Nukers scheme. Here we focus on the 29 core galaxies (hereafter CoreG). We used HST and Chandra data to isolate their optical and X-ray nuclear emission. The CoreG invariably host radio-loud nuclei, with an average radio-loudness parameter of Log R = L5 {GHz} / LB ˜ 3.6. The optical and X-ray nuclear luminosities correlate with the radio-core power, smoothly extending the analogous correlations already found for low luminosity radio-galaxies (LLRG) toward even lower power, by a factor of ˜ 1000, covering a combined range of 6 orders of magnitude. This supports the interpretation of a common non-thermal origin of the nuclear emission also for CoreG. The luminosities of the nuclear sources, most likely dominated by jet emission, set firm upper limits, as low as L/L_Edd ˜ 10-9 in both the optical and X-ray band, on any emission from the accretion process. The similarity of CoreG and LLRG when considering the distributions host galaxies luminosities and black hole masses, as well as of the surface brightness profiles, indicates that they are drawn from the same population of early-type galaxies. LLRG represent only the tip of the iceberg associated with (relatively) high activity levels, with CoreG forming the bulk of the population. We do not find any relationship between radio-power and black hole mass. A minimum black hole mass of M_BH = 108 M⊙ is apparently associated with the radio-loud nuclei in both CoreG and LLRG, but this effect must be tested on a sample of less luminous galaxies, likely to host smaller black holes. In the unifying model for BL Lacs and radio-galaxies, CoreG likely represent the counterparts of the large population of low luminosity BL Lac now emerging from the surveys at low radio flux limits. This suggests the presence of relativistic jets also in these quasi-quiescent early-type "core" galaxies.

Experimental investigations of a uranium plasma pertinent to a self-sustaining plasma source

NASA Technical Reports Server (NTRS)

Schneider, R. T.

1971-01-01

The research is pertinent to the realization of a self-sustained fissioning plasma for applications such as nuclear propulsion, closed cycle MHD power generation using a plasma core reactor, and heat engines such as the nuclear piston engine, as well as the direct conversion of fission energy into optical radiation (nuclear pumped lasers). Diagnostic measurement methods and experimental devices simulating plasma core reactor conditions are discussed. Studies on the following topics are considered: (1) ballistic piston compressor (U-235); (2) high pressure uranium plasma (natural uranium); (3) sliding spark discharge (natural uranium); (4) fission fragment interaction (He-3 and U-235); and (5) nuclear pumped lasers (He-3 and U-235).

A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs

PubMed Central

Gromadzka, Agnieszka M.; Steckelberg, Anna-Lena; Singh, Kusum K.; Hofmann, Kay; Gehring, Niels H.

2016-01-01

The export of messenger RNAs (mRNAs) is the final of several nuclear posttranscriptional steps of gene expression. The formation of export-competent mRNPs involves the recruitment of export factors that are assumed to facilitate transport of the mature mRNAs. Using in vitro splicing assays, we show that a core set of export factors, including ALYREF, UAP56 and DDX39, readily associate with the spliced RNAs in an EJC (exon junction complex)- and cap-dependent manner. In order to elucidate how ALYREF and other export adaptors mediate mRNA export, we conducted a computational analysis and discovered four short, conserved, linear motifs present in RNA-binding proteins. We show that mutation in one of the new motifs (WxHD) in an unstructured region of ALYREF reduced RNA binding and abolished the interaction with eIF4A3 and CBP80. Additionally, the mutation impaired proper localization to nuclear speckles and export of a spliced reporter mRNA. Our results reveal important details of the orchestrated recruitment of export factors during the formation of export competent mRNPs. PMID:26773052

DOE Office of Scientific and Technical Information (OSTI.GOV)

Montemayor, Eric J.; Didychuk, Allison L.; Liao, Honghong

U6 small nuclear RNA (snRNA) is a key component of the active site of the spliceosome, a large ribonucleoprotein complex that catalyzes the splicing of precursor messenger RNA. Prior to its incorporation into the spliceosome, U6 is bound by the protein Prp24, which facilitates unwinding of the U6 internal stem-loop (ISL) so that it can pair with U4 snRNA. A previously reported crystal structure of the `core' of the U6 small nuclear ribonucleoprotein (snRNP) contained an ISL-stabilized A62G mutant of U6 bound to all four RNA-recognition motif (RRM) domains of Prp24 [Montemayoret al.(2014),Nature Struct. Mol. Biol.21, 544–551]. The structure revealedmore » a novel topology containing interlocked rings of protein and RNA that was not predicted by prior biochemical and genetic data. Here, the crystal structure of the U6 snRNP core with a wild-type ISL is reported. This complex crystallized in a new space group, apparently owing in part to the presence of an intramolecular cross-link in RRM1 that was not observed in the previously reported U6-A62G structure. The structure exhibits the same protein–RNA interface and maintains the unique interlocked topology. However, the orientation of the wild-type ISL is altered relative to the A62G mutant structure, suggesting inherent structural dynamics that may facilitate its pairing with U4. Consistent with their similar architectures in the crystalline state, the wild-type and A62G variants of U6 exhibit similar Prp24-binding affinities and electrophoretic mobilities when analyzed by gel-shift assay.« less

Double-clad nuclear fuel safety rod

DOEpatents

McCarthy, William H.; Atcheson, Donald B.; Vaidyanathan, Swaminathan

1984-01-01

A device for shutting down a nuclear reactor during an undercooling or overpower event, whether or not the reactor's scram system operates properly. This is accomplished by double-clad fuel safety rods positioned at various locations throughout the reactor core, wherein melting of a secondary internal cladding of the rod allows the fuel column therein to shift from the reactor core to place the reactor in a subcritical condition.

Double-clad nuclear-fuel safety rod

DOEpatents

McCarthy, W.H.; Atcheson, D.B.

1981-12-30

A device for shutting down a nuclear reactor during an undercooling or overpower event, whether or not the reactor's scram system operates properly. This is accomplished by double-clad fuel safety rods positioned at various locations throughout the reactor core, wherein melting of a secondary internal cladding of the rod allows the fuel column therein to shift from the reactor core to place the reactor in a subcritical condition.

Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors.

PubMed

Becker, Thomas; Pfannschmidt, Sylvia; Guiard, Bernard; Stojanovski, Diana; Milenkovic, Dusanka; Kutik, Stephan; Pfanner, Nikolaus; Meisinger, Chris; Wiedemann, Nils

2008-01-04

The translocase of the outer membrane (TOM complex) is the central entry gate for nuclear-encoded mitochondrial precursor proteins. All Tom proteins are also encoded by nuclear genes and synthesized as precursors in the cytosol. The channel-forming beta-barrel protein Tom40 is targeted to mitochondria via Tom receptors and inserted into the outer membrane by the sorting and assembly machinery (SAM complex). A further outer membrane protein, Mim1, plays a less defined role in assembly of Tom40 into the TOM complex. The three receptors Tom20, Tom22, and Tom70 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N terminus in the case of Tom20 and Tom70 (signal-anchored) or in the C-terminal portion in the case of Tom22 (tail-anchored). Insertion of the precursor of Tom22 into the outer membrane requires pre-existing Tom receptors while the import pathway of the precursors of Tom20 and Tom70 is only poorly understood. We report that Mim1 is required for efficient membrane insertion and assembly of Tom20 and Tom70, but not Tom22. We show that Mim1 associates with SAM(core) components to a large SAM complex, explaining its role in late steps of the assembly pathway of Tom40. We conclude that Mim1 is not only required for biogenesis of the beta-barrel protein Tom40 but also for membrane insertion and assembly of signal-anchored Tom receptors. Thus, Mim1 plays an important role in the efficient assembly of the mitochondrial TOM complex.

Dependence of weak interaction rates on the nuclear composition during stellar core collapse

NASA Astrophysics Data System (ADS)

Furusawa, Shun; Nagakura, Hiroki; Sumiyoshi, Kohsuke; Kato, Chinami; Yamada, Shoichi

2017-02-01

We investigate the influences of the nuclear composition on the weak interaction rates of heavy nuclei during the core collapse of massive stars. The nuclear abundances in nuclear statistical equilibrium (NSE) are calculated by some equation of state (EOS) models including in-medium effects on nuclear masses. We systematically examine the sensitivities of electron capture and neutrino-nucleus scattering on heavy nuclei to the nuclear shell effects and the single-nucleus approximation. We find that the washout of the shell effect at high temperatures brings significant change to weak rates by smoothing the nuclear abundance distribution: the electron capture rate decreases by ˜20 % in the early phase and increases by ˜40 % in the late phase at most, while the cross section for neutrino-nucleus scattering is reduced by ˜15 % . This is because the open-shell nuclei become abundant instead of those with closed neutron shells as the shell effects disappear. We also find that the single-nucleus description based on the average values leads to underestimations of weak rates. Electron captures and neutrino coherent scattering on heavy nuclei are reduced by ˜80 % in the early phase and by ˜5 % in the late phase, respectively. These results indicate that NSE like EOS accounting for shell washout is indispensable for the reliable estimation of weak interaction rates in simulations of core-collapse supernovae.

Fractal analysis and its impact factors on pore structure of artificial cores based on the images obtained using magnetic resonance imaging

NASA Astrophysics Data System (ADS)

Wang, Heming; Liu, Yu; Song, Yongchen; Zhao, Yuechao; Zhao, Jiafei; Wang, Dayong

2012-11-01

Pore structure is one of important factors affecting the properties of porous media, but it is difficult to describe the complexity of pore structure exactly. Fractal theory is an effective and available method for quantifying the complex and irregular pore structure. In this paper, the fractal dimension calculated by box-counting method based on fractal theory was applied to characterize the pore structure of artificial cores. The microstructure or pore distribution in the porous material was obtained using the nuclear magnetic resonance imaging (MRI). Three classical fractals and one sand packed bed model were selected as the experimental material to investigate the influence of box sizes, threshold value, and the image resolution when performing fractal analysis. To avoid the influence of box sizes, a sequence of divisors of the image was proposed and compared with other two algorithms (geometric sequence and arithmetic sequence) with its performance of partitioning the image completely and bringing the least fitted error. Threshold value selected manually and automatically showed that it plays an important role during the image binary processing and the minimum-error method can be used to obtain an appropriate or reasonable one. Images obtained under different pixel matrices in MRI were used to analyze the influence of image resolution. Higher image resolution can detect more quantity of pore structure and increase its irregularity. With benefits of those influence factors, fractal analysis on four kinds of artificial cores showed the fractal dimension can be used to distinguish the different kinds of artificial cores and the relationship between fractal dimension and porosity or permeability can be expressed by the model of D = a - bln(x + c).

Thermal barrier and support for nuclear reactor fuel core

DOEpatents

Betts, Jr., William S.; Pickering, J. Larry; Black, William E.

1987-01-01

A thermal barrier/core support for the fuel core of a nuclear reactor having a metallic cylinder secured to the reactor vessel liner and surrounded by fibrous insulation material. A top cap is secured to the upper end of the metallic cylinder that locates and orients a cover block and post seat. Under normal operating conditions, the metallic cylinder supports the entire load exerted by its associated fuel core post. Disposed within the metallic cylinder is a column of ceramic material, the height of which is less than that of the metallic cylinder, and thus is not normally load bearing. In the event of a temperature excursion beyond the design limits of the metallic cylinder and resulting in deformation of the cylinder, the ceramic column will abut the top cap to support the fuel core post.

Evolution of an RNP assembly system: A minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster

PubMed Central

Kroiss, Matthias; Schultz, Jörg; Wiesner, Julia; Chari, Ashwin; Sickmann, Albert; Fischer, Utz

2008-01-01

In vertebrates, assembly of spliceosomal uridine-rich small nuclear ribonucleoproteins (UsnRNPs) is mediated by the SMN complex, a macromolecular entity composed of the proteins SMN and Gemins 2–8. Here we have studied the evolution of this machinery using complete genome assemblies of multiple model organisms. The SMN complex has gained complexity in evolution by a blockwise addition of Gemins onto an ancestral core complex composed of SMN and Gemin2. In contrast to this overall evolutionary trend to more complexity in metazoans, orthologs of most Gemins are missing in dipterans. In accordance with these bioinformatic data a previously undescribed biochemical purification strategy elucidated that the dipteran Drosophila melanogaster contains an SMN complex of remarkable simplicity. Surprisingly, this minimal complex not only mediates the assembly reaction in a manner very similar to its vertebrate counterpart, but also prevents misassembly onto nontarget RNAs. Our data suggest that only a minority of Gemins are required for the assembly reaction per se, whereas others may serve additional functions in the context of UsnRNP biogenesis. The evolution of the SMN complex is an interesting example of how the simplification of a biochemical process contributes to genome compaction. PMID:18621711

78 FR 63516 - Initial Test Program of Emergency Core Cooling Systems for New Boiling-Water Reactors

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-24

... NUCLEAR REGULATORY COMMISSION [NRC-2012-0134] Initial Test Program of Emergency Core Cooling....79.1, ``Initial Test Program of Emergency Core Cooling Systems for New Boiling-Water Reactors.'' This... emergency core cooling systems (ECCSs) for boiling- water reactors (BWRs) whose licenses are issued after...

Structural basis for MTR4-ZCCHC8 interactions that stimulate the MTR4 helicase in the nuclear exosome-targeting complex.

PubMed

Puno, M Rhyan; Lima, Christopher D

2018-06-12

The nuclear exosome-targeting (NEXT) complex functions as an RNA exosome cofactor and is involved in surveillance and turnover of aberrant transcripts and noncoding RNAs. NEXT is a ternary complex composed of the RNA-binding protein RBM7, the scaffold zinc-knuckle protein ZCCHC8, and the helicase MTR4. While RNA interactions with RBM7 are known, it remains unclear how NEXT subunits collaborate to recognize and prepare substrates for degradation. Here, we show that MTR4 helicase activity is enhanced when associated with RBM7 and ZCCHC8. While uridine-rich substrates interact with RBM7 and are preferred, optimal activity is observed when substrates include a polyadenylated 3' end. We identify a bipartite interaction of ZCCHC8 with MTR4 and uncover a role for the conserved C-terminal domain of ZCCHC8 in stimulating MTR4 helicase and ATPase activities. A crystal structure reveals that the ZCCHC8 C-terminal domain binds the helicase core in a manner that is distinct from that observed for Saccharomyces cerevisiae exosome cofactors Trf4p and Air2p. Our results are consistent with a model whereby effective targeting of substrates by NEXT entails recognition of elements within the substrate and activation of MTR4 helicase activity. Copyright © 2018 the Author(s). Published by PNAS.

Understanding Core-Collapse Supernovae

NASA Astrophysics Data System (ADS)

Hix, W. R.; Lentz, E. J.; Baird, M.; Messer, O. E. B.; Mezzacappa, A.; Lee, C.-T.; Bruenn, S. W.; Blondin, J. M.; Marronetti, P.

2010-03-01

Our understanding of core-collapse supernovae continues to improve as better microphysics is included in increasingly realistic neutrino-radiationhydrodynamic simulations. Recent multi-dimensional models with spectral neutrino transport, which slowly develop successful explosions for a range of progenitors between 12 and 25 solar mass, have motivated changes in our understanding of the neutrino reheating mechanism. In a similar fashion, improvements in nuclear physics, most notably explorations of weak interactions on nuclei and the nuclear equation of state, continue to refine our understanding of how supernovae explode. Recent progresses on both the macroscopic and microscopic effects that affect core-collapse supernovae are discussed.

10 CFR 50.46 - Acceptance criteria for emergency core cooling systems for light-water nuclear power reactors.

Code of Federal Regulations, 2014 CFR

2014-01-01

... light-water nuclear power reactors. 50.46 Section 50.46 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC... reactors. (a)(1)(i) Each boiling or pressurized light-water nuclear power reactor fueled with uranium oxide... evaluation model. This section does not apply to a nuclear power reactor facility for which the...

10 CFR Appendix A to Part 110 - Illustrative List of Nuclear Reactor Equipment Under NRC Export Licensing Authority

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Illustrative List of Nuclear Reactor Equipment Under NRC... List of Nuclear Reactor Equipment Under NRC Export Licensing Authority Note: A nuclear reactor... core of a nuclear reactor and capable of withstanding the operating pressure of the primary coolant. (2...

10 CFR 50.46 - Acceptance criteria for emergency core cooling systems for light-water nuclear power reactors.

Code of Federal Regulations, 2013 CFR

2013-01-01

... light-water nuclear power reactors. 50.46 Section 50.46 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC... reactors. (a)(1)(i) Each boiling or pressurized light-water nuclear power reactor fueled with uranium oxide... evaluation model. This section does not apply to a nuclear power reactor facility for which the...

10 CFR 50.46 - Acceptance criteria for emergency core cooling systems for light-water nuclear power reactors.

Code of Federal Regulations, 2012 CFR

2012-01-01

... light-water nuclear power reactors. 50.46 Section 50.46 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC... reactors. (a)(1)(i) Each boiling or pressurized light-water nuclear power reactor fueled with uranium oxide... evaluation model. This section does not apply to a nuclear power reactor facility for which the...

Characterizing core-periphery structure of complex network by h-core and fingerprint curve

NASA Astrophysics Data System (ADS)

Li, Simon S.; Ye, Adam Y.; Qi, Eric P.; Stanley, H. Eugene; Ye, Fred Y.

2018-02-01

It is proposed that the core-periphery structure of complex networks can be simulated by h-cores and fingerprint curves. While the features of core structure are characterized by h-core, the features of periphery structure are visualized by rose or spiral curve as the fingerprint curve linking to entire-network parameters. It is suggested that a complex network can be approached by h-core and rose curves as the first-order Fourier-approach, where the core-periphery structure is characterized by five parameters: network h-index, network radius, degree power, network density and average clustering coefficient. The simulation looks Fourier-like analysis.

Nuclear design analysis of square-lattice honeycomb space nuclear rocket engine

NASA Astrophysics Data System (ADS)

Widargo, Reza; Anghaie, Samim

1999-01-01

The square-lattice honeycomb reactor is designed based on a cylindrical core that is determined to have critical diameter and length of 0.50 m and 0.50 c, respectively. A 0.10-cm thick radial graphite reflector, in addition to a 0.20-m thick axial graphite reflector are used to reduce neutron leakage from the reactor. The core is fueled with solid solution of 93% enriched (U, Zr, Nb)C, which is one of several ternary uranium carbides that are considered for this concept. The fuel is to be fabricated as 2 mm grooved (U, Zr, Nb)C wafers. The fuel wafers are used to form square-lattice honeycomb fuel assemblies, 0.10 m in length with 30% cross-sectional flow area. Five fuel assemblies are stacked up axially to form the reactor core. Based on the 30% void fraction, the width of the square flow channel is about 1.3 mm. The hydrogen propellant is passed through these flow channels and removes the heat from the reactor core. To perform nuclear design analysis, a series of neutron transport and diffusion codes are used. The preliminary results are obtained using a simple four-group cross-section model. To optimize the nuclear design, the fuel densities are varied for each assembly. Tantalum, hafnium and tungsten are considered and used as a replacement for niobium in fuel material to provide water submersion sub-criticality for the reactor. Axial and radial neutron flux and power density distributions are calculated for the core. Results of the neutronic analysis indicate that the core has a relatively fast spectrum. From the results of the thermal hydraulic analyses, eight axial temperature zones are chosen for the calculation of group average cross-sections. An iterative process is conducted to couple the neutronic calculations with the thermal hydraulics calculations. Results of the nuclear design analysis indicate that a compact core can be designed based on ternary uranium carbide square-lattice honeycomb fuel. This design provides a relatively high thrust to weight ratio.

Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation

PubMed Central

Zhang, Yi; Ng, Huck-Hui; Erdjument-Bromage, Hediye; Tempst, Paul; Bird, Adrian; Reinberg, Danny

1999-01-01

ATP-dependent nucleosome remodeling and core histone acetylation and deacetylation represent mechanisms to alter nucleosome structure. NuRD is a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. The histone deacetylases HDAC1 and HDAC2 and the histone binding proteins RbAp48 and RbAp46 form a core complex shared between NuRD and Sin3-histone deacetylase complexes. The histone deacetylase activity of the core complex is severely compromised. A novel polypeptide highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain-containing protein, MBD3, were found to be subunits of the NuRD complex. MTA2 modulates the enzymatic activity of the histone deacetylase core complex. MBD3 mediates the association of MTA2 with the core histone deacetylase complex. MBD3 does not directly bind methylated DNA but is highly related to MBD2, a polypeptide that binds to methylated DNA and has been reported to possess demethylase activity. MBD2 interacts with the NuRD complex and directs the complex to methylated DNA. NuRD may provide a means of gene silencing by DNA methylation. PMID:10444591

A new equation of state Based on Nuclear Statistical Equilibrium for Core-Collapse Simulations

NASA Astrophysics Data System (ADS)

Furusawa, Shun; Yamada, Shoichi; Sumiyoshi, Kohsuke; Suzuki, Hideyuki

2012-09-01

We calculate a new equation of state for baryons at sub-nuclear densities for the use in core-collapse simulations of massive stars. The formulation is the nuclear statistical equilibrium description and the liquid drop approximation of nuclei. The model free energy to minimize is calculated by relativistic mean field theory for nucleons and the mass formula for nuclei with atomic number up to ~ 1000. We have also taken into account the pasta phase. We find that the free energy and other thermodynamical quantities are not very different from those given in the standard EOSs that adopt the single nucleus approximation. On the other hand, the average mass is systematically different, which may have an important effect on the rates of electron captures and coherent neutrino scatterings on nuclei in supernova cores.

Dual annular rotating [open quotes]windowed[close quotes] nuclear reflector reactor control system

DOEpatents

Jacox, M.G.; Drexler, R.L.; Hunt, R.N.M.; Lake, J.A.

1994-03-29

A nuclear reactor control system is provided in a nuclear reactor having a core operating in the fast neutron energy spectrum where criticality control is achieved by neutron leakage. The control system includes dual annular, rotatable reflector rings. There are two reflector rings: an inner reflector ring and an outer reflector ring. The reflectors are concentrically assembled, surround the reactor core, and each reflector ring includes a plurality of openings. The openings in each ring are capable of being aligned or non-aligned with each other. Independent driving means for each of the annular reflector rings is provided so that reactor criticality can be initiated and controlled by rotation of either reflector ring such that the extent of alignment of the openings in each ring controls the reflection of neutrons from the core. 4 figures.

POWER GENERATION FROM LIQUID METAL NUCLEAR FUEL

DOEpatents

Dwyer, O.E.

1958-12-23

A nuclear reactor system is described wherein the reactor is the type using a liquid metal fuel, such as a dispersion of fissile material in bismuth. The reactor is designed ln the form of a closed loop having a core sectlon and heat exchanger sections. The liquid fuel is clrculated through the loop undergoing flssion in the core section to produce heat energy and transferrlng this heat energy to secondary fluids in the heat exchanger sections. The fission in the core may be produced by a separate neutron source or by a selfsustained chain reaction of the liquid fuel present in the core section. Additional auxiliary heat exchangers are used in the system to convert water into steam which drives a turbine.

Biogenesis of light harvesting proteins.

PubMed

Dall'Osto, Luca; Bressan, Mauro; Bassi, Roberto

2015-09-01

The LHC family includes nuclear-encoded, integral thylakoid membrane proteins, most of which coordinate chlorophyll and xanthophyll chromophores. By assembling with the core complexes of both photosystems, LHCs form a flexible peripheral moiety for enhancing light-harvesting cross-section, regulating its efficiency and providing protection against photo-oxidative stress. Upon its first appearance, LHC proteins underwent evolutionary diversification into a large protein family with a complex genetic redundancy. Such differentiation appears as a crucial event in the adaptation of photosynthetic organisms to changing environmental conditions and land colonization. The structure of photosystems, including nuclear- and chloroplast-encoded subunits, presented the cell with a number of challenges for the control of the light harvesting function. Indeed, LHC-encoding messages are translated in the cytosol, and pre-proteins imported into the chloroplast, processed to their mature size and targeted to the thylakoids where are assembled with chromophores. Thus, a tight coordination between nuclear and plastid gene expression, in response to environmental stimuli, is required to adjust LHC composition during photoacclimation. In recent years, remarkable progress has been achieved in elucidating structure, function and regulatory pathways involving LHCs; however, a number of molecular details still await elucidation. In this review, we will provide an overview on the current knowledge on LHC biogenesis, ranging from organization of pigment-protein complexes to the modulation of gene expression, import and targeting to the photosynthetic membranes, and regulation of LHC assembly and turnover. Genes controlling these events are potential candidate for biotechnological applications aimed at optimizing light use efficiency of photosynthetic organisms. This article is part of a Special Issue entitled: Chloroplast biogenesis. Copyright © 2015 Elsevier B.V. All rights reserved.

Thermionic nuclear reactor with internal heat distribution and multiple duct cooling

DOEpatents

Fisher, C.R.; Perry, L.W. Jr.

1975-11-01

A Thermionic Nuclear Reactor is described having multiple ribbon-like coolant ducts passing through the core, intertwined among the thermionic fuel elements to provide independent cooling paths. Heat pipes are disposed in the core between and adjacent to the thermionic fuel elements and the ribbon ducting, for the purpose of more uniformly distributing the heat of fission among the thermionic fuel elements and the ducts.

FUEL ELEMENTS FOR THERMAL-FISSION NUCLEAR REACTORS

DOEpatents

Flint, O.

1961-01-10

Fuel elements for thermal-fission nuclear reactors are described. The fuel element is comprised of a core of alumina, a film of a metal of the class consisting of copper, silver, and nickel on the outer face of the core, and a coating of an oxide of a metal isotope of the class consisting of Un/sup 235/, U/ sup 233/, and Pu/sup 239/ on the metal f ilm.

An historical collection of papers on nuclear thermal propulsion

NASA Astrophysics Data System (ADS)

The present volume of historical papers on nuclear thermal propulsion (NTP) encompasses NTP technology development regarding solid-core NTP technology, advanced concepts from the early years of NTP research, and recent activities in the field. Specific issues addressed include NERVA rocket-engine technology, the development of nuclear rocket propulsion at Los Alamos, fuel-element development, reactor testing for the Rover program, and an overview of NTP concepts and research emphasizing two decades of NASA research. Also addressed are the development of the 'nuclear light bulb' closed-cycle gas core and a demonstration of a fissioning UF6 gas in an argon vortex. The recent developments reviewed include the application of NTP to NASA's Lunar Space Transportation System, the use of NTP for the Space Exploration Initiative, and the development of nuclear rocket engines in the former Soviet Union.

ELECTRON-CAPTURE AND β-DECAY RATES FOR sd-SHELL NUCLEI IN STELLAR ENVIRONMENTS RELEVANT TO HIGH-DENSITY O–NE–MG CORES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suzuki, Toshio; Toki, Hiroshi; Nomoto, Ken’ichi, E-mail: suzuki@phys.chs.nihon-u.ac.jp

Electron-capture and β-decay rates for nuclear pairs in the sd-shell are evaluated at high densities and high temperatures relevant to the final evolution of electron-degenerate O–Ne–Mg cores of stars with initial masses of 8–10 M{sub ⊙}. Electron capture induces a rapid contraction of the electron-degenerate O–Ne–Mg core. The outcome of rapid contraction depends on the evolutionary changes in the central density and temperature, which are determined by the competing processes of contraction, cooling, and heating. The fate of the stars is determined by these competitions, whether they end up with electron-capture supernovae or Fe core-collapse supernovae. Since the competing processes aremore » induced by electron capture and β-decay, the accurate weak rates are crucially important. The rates are obtained for pairs with A = 20, 23, 24, 25, and 27 by shell-model calculations in the sd-shell with the USDB Hamiltonian. Effects of Coulomb corrections on the rates are evaluated. The rates for pairs with A = 23 and 25 are important for nuclear Urca processes that determine the cooling rate of the O–Ne–Mg core, while those for pairs with A = 20 and 24 are important for the core contraction and heat generation rates in the core. We provide these nuclear rates at stellar environments in tables with fine enough meshes at various densities and temperatures for studies of astrophysical processes sensitive to the rates. In particular, the accurate rate tables are crucially important for the final fates of not only O–Ne–Mg cores but also a wider range of stars, such as C–O cores of lower-mass stars.« less

Melting and vibrational properties of planetary materials under deep Earth conditions

NASA Astrophysics Data System (ADS)

Jackson, Jennifer

2013-06-01

The large chemical, density, and dynamical contrasts associated with the juxtaposition of a liquid iron-dominant alloy and silicates at Earth's core-mantle boundary (CMB) are associated with a rich range of complex seismological features. For example, seismic heterogeneity at this boundary includes small patches of anomalously low sound velocities, called ultralow-velocity zones. Their small size (5 to 40 km thick) and depth (about 2800 km) present unique challenges for seismic characterization and geochemical interpretation. In this contribution, we will present recent nuclear resonant inelastic x-ray scattering measurements on iron-bearing silicates, oxides, and metals, and their application towards our understanding of Earth's interior. Specifically, we will present measurements on silicates and oxide minerals that are important in Earth's upper and lower mantles, as well as iron to over 1 megabar in pressure. The nuclear resonant inelastic x-ray scattering method provides specific vibrational information, e.g., the phonon density of states, and in combination with compression data permits the determination of sound velocities and other vibrational information under high pressure and high temperature. For example, accurate determination of the sound velocities and density of chemically complex Earth materials is essential for understanding the distribution and behavior of minerals and iron-alloys with depth. The high statistical quality of the data in combination with high energy resolution and a small x-ray focus size permit accurate evaluation of the vibrational-related quantities of iron-bearing Earth materials as a function of pressure, such as the Grüneisen parameter, thermal pressure, sound velocities, and iron isotope fractionation quantities. Finally, we will present a novel method detecting the solid-liquid phase boundary of compressed iron at high temperatures using synchrotron Mössbauer spectroscopy. Our approach is unique because the dynamics of the iron atoms are monitored. This process is described by the Lamb-Mössbauer factor, which is related to the mean-square displacement of the iron atoms. We will discuss the implications of our results as they relate to Earth's core and core-mantle boundary regions.

Enhancing oxidative stability in heated oils using core/shell structures of collagen and α-tocopherol complex.

PubMed

Gim, Seo Yeong; Hong, Seungmi; Kim, Jisu; Kwon, YongJun; Kim, Mi-Ja; Kim, GeunHyung; Lee, JaeHwan

2017-11-15

In this study, collagen mesh structure was prepared by carrying α-tocopherol in the form of core/shell complex. Antioxidant properties of α-tocopherol loaded carriers were tested in moisture added bulk oils at 140°C. From one gram of collagen core/shell complex, 138mg α-tocopherol was released in medium chain triacylglycerol (MCT). α-Tocopherol was substantially protected against heat treatment when α-tocopherol was complexed in collagen core/shell. Oxidative stability in bulk oil was significantly enhanced by added collagen mesh structure or collagen core/shell complex with α-tocopherol compared to that in control bulk oils (p<0.05), although no significant difference was observed between oils containing collagen mesh structure and collagen core/shell with α-tocopherol (p>0.05). Results of DPPH loss in methanol demonstrated that collagen core/shell with α-tocopherol had significantly (p<0.05) higher antioxidant properties than collagen mesh structure up to a certain period. Therefore, collagen core/shell complex is a promising way to enhance the stability of α-tocopherol and oxidative stability in oil-rich foods prepared at high temperature. Copyright © 2017 Elsevier Ltd. All rights reserved.

Inhibition of U snRNP assembly by a virus-encoded proteinase.

PubMed

Almstead, Laura L; Sarnow, Peter

2007-05-01

It has been proposed that defects in the assembly of spliceosomal uridine-rich small nuclear ribonucleoprotein (U snRNP) complexes could account for the death of motor neurons in spinal muscular atrophy (SMA). We discovered that infection of cultured cells with poliovirus results in the specific cleavage of the host factor Gemin3 by a virus-encoded proteinase, 2A(pro). Gemin3 is a component of the macromolecular SMN complex that mediates assembly of U snRNP complexes by aiding the heptameric oligomerization of Sm proteins onto U snRNAs. Using in vitro Sm core assembly assays, we found that lowering the intracellular amounts of Gemin3 by either poliovirus infection or small interfering RNA (siRNA)-mediated knockdown of Gemin3 resulted in reduced assembly of U snRNPs. Immunofluorescence analyses revealed a specific redistribution of Sm proteins from the nucleoplasm to the cytoplasmic periphery of the nucleus in poliovirus-infected cells. We propose that defects in U snRNP assembly may be shared features of SMA and poliomyelitis.

The effect of peculiar complex core balance training on isokinetic muscle functions of the knee and lumbus.

PubMed

Lee, Myungsun; Han, Gunsoo

2016-04-01

[Purpose] This study aimed to investigate the effect of peculiar complex core balance training on the isokinetic muscle function of the knee joint and lumbus to provide fundamental data for establishing a training program that focuses on improving the performance and prevention of injury by developing the core and low extremity muscles. [Subjects and Methods] The participants in this study included a total of ten high school athletes involved in a throwing event for over five years. The subjects were randomly divided into two groups: The experimental group (N=5) and the control group (N=5). The experimental group underwent peculiar complex core balance training. [Results] According to the analysis of covariance, there was a significant effect of peculiar complex core balance training. Therefore, the isokinetic muscle function of the knee joint and lumbus in the experimental group participating in peculiar complex core balance training was significantly increased compared to the control group. [Conclusion] It is concluded that peculiar complex core balance training had a positive effect on the isokinetic muscle function of the knee and lumbus in throwing event athletes.

NEW EQUATIONS OF STATE IN SIMULATIONS OF CORE-COLLAPSE SUPERNOVAE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hempel, M.; Liebendoerfer, M.; Fischer, T.

2012-03-20

We discuss three new equations of state (EOS) in core-collapse supernova simulations. The new EOS are based on the nuclear statistical equilibrium model of Hempel and Schaffner-Bielich (HS), which includes excluded volume effects and relativistic mean-field (RMF) interactions. We consider the RMF parameterizations TM1, TMA, and FSUgold. These EOS are implemented into our spherically symmetric core-collapse supernova model, which is based on general relativistic radiation hydrodynamics and three-flavor Boltzmann neutrino transport. The results obtained for the new EOS are compared with the widely used EOS of H. Shen et al. and Lattimer and Swesty. The systematic comparison shows that themore » model description of inhomogeneous nuclear matter is as important as the parameterization of the nuclear interactions for the supernova dynamics and the neutrino signal. Furthermore, several new aspects of nuclear physics are investigated: the HS EOS contains distributions of nuclei, including nuclear shell effects. The appearance of light nuclei, e.g., deuterium and tritium, is also explored, which can become as abundant as alphas and free protons. In addition, we investigate the black hole formation in failed core-collapse supernovae, which is mainly determined by the high-density EOS. We find that temperature effects lead to a systematically faster collapse for the non-relativistic LS EOS in comparison with the RMF EOS. We deduce a new correlation for the time until black hole formation, which allows the determination of the maximum mass of proto-neutron stars, if the neutrino signal from such a failed supernova would be measured in the future. This would give a constraint for the nuclear EOS at finite entropy, complementary to observations of cold neutron stars.« less

Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

PubMed

Pasion, S G; Forsburg, S L

1999-12-01

The minichromosome maintenance (MCM) proteins MCM2-MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear throughout the cell cycle. In studying the mechanism that regulates assembly of the MCM complex, we analyzed the cis and trans elements required for nuclear localization of a single subunit, Mcm2p. Mutation of any single mcm gene leads to redistribution of wild-type MCM subunits to the cytoplasm, and this redistribution depends on an active nuclear export system. We identified the nuclear localization signal sequences of Mcm2p and showed that these are required for nuclear targeting of other MCM subunits. In turn, Mcm2p must associate with other MCM proteins for its proper localization; nuclear localization of MCM proteins thus requires assembly of MCM proteins in a complex. We suggest that coupling complex assembly to nuclear targeting and retention ensures that only intact heterohexameric MCM complexes remain nuclear.

Nuclear Localization of Schizosaccharomyces pombe Mcm2/Cdc19p Requires MCM Complex Assembly

PubMed Central

Pasion, Sally G.; Forsburg, Susan L.

1999-01-01

The minichromosome maintenance (MCM) proteins MCM2–MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear throughout the cell cycle. In studying the mechanism that regulates assembly of the MCM complex, we analyzed the cis and trans elements required for nuclear localization of a single subunit, Mcm2p. Mutation of any single mcm gene leads to redistribution of wild-type MCM subunits to the cytoplasm, and this redistribution depends on an active nuclear export system. We identified the nuclear localization signal sequences of Mcm2p and showed that these are required for nuclear targeting of other MCM subunits. In turn, Mcm2p must associate with other MCM proteins for its proper localization; nuclear localization of MCM proteins thus requires assembly of MCM proteins in a complex. We suggest that coupling complex assembly to nuclear targeting and retention ensures that only intact heterohexameric MCM complexes remain nuclear. PMID:10588642

Identifying Dynamic Protein Complexes Based on Gene Expression Profiles and PPI Networks

PubMed Central

Li, Min; Chen, Weijie; Wang, Jianxin; Pan, Yi

2014-01-01

Identification of protein complexes from protein-protein interaction networks has become a key problem for understanding cellular life in postgenomic era. Many computational methods have been proposed for identifying protein complexes. Up to now, the existing computational methods are mostly applied on static PPI networks. However, proteins and their interactions are dynamic in reality. Identifying dynamic protein complexes is more meaningful and challenging. In this paper, a novel algorithm, named DPC, is proposed to identify dynamic protein complexes by integrating PPI data and gene expression profiles. According to Core-Attachment assumption, these proteins which are always active in the molecular cycle are regarded as core proteins. The protein-complex cores are identified from these always active proteins by detecting dense subgraphs. Final protein complexes are extended from the protein-complex cores by adding attachments based on a topological character of “closeness” and dynamic meaning. The protein complexes produced by our algorithm DPC contain two parts: static core expressed in all the molecular cycle and dynamic attachments short-lived. The proposed algorithm DPC was applied on the data of Saccharomyces cerevisiae and the experimental results show that DPC outperforms CMC, MCL, SPICi, HC-PIN, COACH, and Core-Attachment based on the validation of matching with known complexes and hF-measures. PMID:24963481

Mathematical Modeling Of A Nuclear/Thermionic Power Source

NASA Technical Reports Server (NTRS)

Vandersande, Jan W.; Ewell, Richard C.

1992-01-01

Report discusses mathematical modeling to predict performance and lifetime of spacecraft power source that is integrated combination of nuclear-fission reactor and thermionic converters. Details of nuclear reaction, thermal conditions in core, and thermionic performance combined with model of swelling of fuel.

Neutrino-pair emission from nuclear de-excitation in core-collapse supernova simulations

NASA Astrophysics Data System (ADS)

Fischer, T.; Langanke, K.; Martínez-Pinedo, G.

2013-12-01

We study the impact of neutrino-pair production from the de-excitation of highly excited heavy nuclei on core-collapse supernova simulations, following the evolution up to several 100 ms after core bounce. Our study is based on the agile-boltztransupernova code, which features general relativistic radiation hydrodynamics and accurate three-flavor Boltzmann neutrino transport in spherical symmetry. In our simulations the nuclear de-excitation process is described in two different ways. At first we follow the approach proposed by Fuller and Meyer [Astrophys. J.AJLEEY0004-637X10.1086/170317 376, 701 (1991)], which is based on strength functions derived in the framework of the nuclear Fermi-gas model of noninteracting nucleons. Second, we parametrize the allowed and forbidden strength distributions in accordance with measurements for selected nuclear ground states. We determine the de-excitation strength by applying the Brink hypothesis and detailed balance. For both approaches, we find that nuclear de-excitation has no effect on the supernova dynamics. However, we find that nuclear de-excitation is the leading source for the production of electron antineutrinos as well as heavy-lepton-flavor (anti)neutrinos during the collapse phase. At sufficiently high densities, the associated neutrino spectra are influenced by interactions with the surrounding matter, making proper simulations of neutrino transport important for the determination of the neutrino-energy loss rate. We find that, even including nuclear de-excitations, the energy loss during the collapse phase is overwhelmingly dominated by electron neutrinos produced by electron capture.

Structures of p -shell double-Λ hypernuclei studied with microscopic cluster models

NASA Astrophysics Data System (ADS)

Kanada-En'yo, Yoshiko

2018-03-01

0 s -orbit Λ states in p -shell double-Λ hypernuclei (Z Λ Λ A ), Li Λ Λ 8 , Li Λ Λ 9 , Be Λ Λ 10 ,11 ,12 , B Λ Λ 12 ,13 , and C Λ Λ 14 are investigated. Microscopic cluster models are applied to core nuclear part and a potential model is adopted for Λ particles. The Λ -core potential is a folding potential obtained with effective G -matrix Λ -N interactions, which reasonably reproduce energy spectra of Z Λ A -1 . System dependence of the Λ -Λ binding energies is understood by the core polarization energy from nuclear size reduction. Reductions of nuclear sizes and E 2 transition strengths by Λ particles are also discussed.

Impact of New Nuclear Data Libraries on Small Sized Long Life CANDLE HTGR Design Parameters

NASA Astrophysics Data System (ADS)

Liem, Peng Hong; Hartanto, Donny; Tran, Hoai Nam

2017-01-01

The impact of new evaluated nuclear data libraries (JENDL-4.0, ENDF/B-VII.0 and JEFF-3.1) on the core characteristics of small-sized long-life CANDLE High Temperature Gas-Cooled Reactors (HTGRs) with uranium and thorium fuel cycles was investigated. The most important parameters of the CANDLE core characteristics investigated here covered (1) infinite multiplication factor of the fresh fuel containing burnable poison, (2) the effective multiplication factor of the equilibrium core, (3) the moving velocity of the burning region, (4) the attained discharge burnup, and (5) the maximum power density. The reference case was taken from the current JENDL-3.3 results. For the uranium fuel cycle, the impact of the new libraries was small, while significant impact was found for thorium fuel cycle. The findings indicated the needs of more accurate nuclear data libraries for nuclides involved in thorium fuel cycle in the future.

An assessment of coupling algorithms for nuclear reactor core physics simulations

DOE PAGES

Hamilton, Steven; Berrill, Mark; Clarno, Kevin; ...

2016-04-01

This paper evaluates the performance of multiphysics coupling algorithms applied to a light water nuclear reactor core simulation. The simulation couples the k-eigenvalue form of the neutron transport equation with heat conduction and subchannel flow equations. We compare Picard iteration (block Gauss–Seidel) to Anderson acceleration and multiple variants of preconditioned Jacobian-free Newton–Krylov (JFNK). The performance of the methods are evaluated over a range of energy group structures and core power levels. A novel physics-based approximation to a Jacobian-vector product has been developed to mitigate the impact of expensive on-line cross section processing steps. Furthermore, numerical simulations demonstrating the efficiency ofmore » JFNK and Anderson acceleration relative to standard Picard iteration are performed on a 3D model of a nuclear fuel assembly. Both criticality (k-eigenvalue) and critical boron search problems are considered.« less

An assessment of coupling algorithms for nuclear reactor core physics simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamilton, Steven; Berrill, Mark; Clarno, Kevin

This paper evaluates the performance of multiphysics coupling algorithms applied to a light water nuclear reactor core simulation. The simulation couples the k-eigenvalue form of the neutron transport equation with heat conduction and subchannel flow equations. We compare Picard iteration (block Gauss–Seidel) to Anderson acceleration and multiple variants of preconditioned Jacobian-free Newton–Krylov (JFNK). The performance of the methods are evaluated over a range of energy group structures and core power levels. A novel physics-based approximation to a Jacobian-vector product has been developed to mitigate the impact of expensive on-line cross section processing steps. Furthermore, numerical simulations demonstrating the efficiency ofmore » JFNK and Anderson acceleration relative to standard Picard iteration are performed on a 3D model of a nuclear fuel assembly. Both criticality (k-eigenvalue) and critical boron search problems are considered.« less

An assessment of coupling algorithms for nuclear reactor core physics simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamilton, Steven, E-mail: hamiltonsp@ornl.gov; Berrill, Mark, E-mail: berrillma@ornl.gov; Clarno, Kevin, E-mail: clarnokt@ornl.gov

This paper evaluates the performance of multiphysics coupling algorithms applied to a light water nuclear reactor core simulation. The simulation couples the k-eigenvalue form of the neutron transport equation with heat conduction and subchannel flow equations. We compare Picard iteration (block Gauss–Seidel) to Anderson acceleration and multiple variants of preconditioned Jacobian-free Newton–Krylov (JFNK). The performance of the methods are evaluated over a range of energy group structures and core power levels. A novel physics-based approximation to a Jacobian-vector product has been developed to mitigate the impact of expensive on-line cross section processing steps. Numerical simulations demonstrating the efficiency of JFNKmore » and Anderson acceleration relative to standard Picard iteration are performed on a 3D model of a nuclear fuel assembly. Both criticality (k-eigenvalue) and critical boron search problems are considered.« less

Experimental Criticality Benchmarks for SNAP 10A/2 Reactor Cores

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krass, A.W.

2005-12-19

This report describes computational benchmark models for nuclear criticality derived from descriptions of the Systems for Nuclear Auxiliary Power (SNAP) Critical Assembly (SCA)-4B experimental criticality program conducted by Atomics International during the early 1960's. The selected experimental configurations consist of fueled SNAP 10A/2-type reactor cores subject to varied conditions of water immersion and reflection under experimental control to measure neutron multiplication. SNAP 10A/2-type reactor cores are compact volumes fueled and moderated with the hydride of highly enriched uranium-zirconium alloy. Specifications for the materials and geometry needed to describe a given experimental configuration for a model using MCNP5 are provided. Themore » material and geometry specifications are adequate to permit user development of input for alternative nuclear safety codes, such as KENO. A total of 73 distinct experimental configurations are described.« less

Development concept for a small, split-core, heat-pipe-cooled nuclear reactor

NASA Technical Reports Server (NTRS)

Lantz, E.; Breitwieser, R.; Niederauer, G. F.

1974-01-01

There have been two main deterrents to the development of semiportable nuclear reactors. One is the high development costs; the other is the inability to satisfy with assurance the questions of operational safety. This report shows how a split-core, heat-pipe cooled reactor could conceptually eliminate these deterrents, and examines and summarizes recent work on split-core, heat-pipe reactors. A concept for a small reactor that could be developed at a comparatively low cost is presented. The concept would extend the technology of subcritical radioisotope thermoelectric generators using 238 PuO2 to the evolution of critical space power reactors using 239 PuO2.

Initial Coupling of the RELAP-7 and PRONGHORN Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

J. Ortensi; D. Andrs; A.A. Bingham

2012-10-01

Modern nuclear reactor safety codes require the ability to solve detailed coupled neutronic- thermal fluids problems. For larger cores, this implies fully coupled higher dimensionality spatial dynamics with appropriate feedback models that can provide enough resolution to accurately compute core heat generation and removal during steady and unsteady conditions. The reactor analysis code PRONGHORN is being coupled to RELAP-7 as a first step to extend RELAP’s current capabilities. This report details the mathematical models, the type of coupling, and the testing results from the integrated system. RELAP-7 is a MOOSE-based application that solves the continuity, momentum, and energy equations inmore » 1-D for a compressible fluid. The pipe and joint capabilities enable it to model parts of the power conversion unit. The PRONGHORN application, also developed on the MOOSE infrastructure, solves the coupled equations that define the neutron diffusion, fluid flow, and heat transfer in a full core model. The two systems are loosely coupled to simplify the transition towards a more complex infrastructure. The integration is tested on a simplified version of the OECD/NEA MHTGR-350 Coupled Neutronics-Thermal Fluids benchmark model.« less

Construction of hybrid photosynthetic units using peripheral and core antennae from two different species of photosynthetic bacteria: detection of the energy transfer from bacteriochlorophyll a in LH2 to bacteriochlorophyll b in LH1.

PubMed

Fujii, Ritsuko; Shimonaka, Shozo; Uchida, Naoko; Gardiner, Alastair T; Cogdell, Richard J; Sugisaki, Mitsuru; Hashimoto, Hideki

2008-01-01

Typical purple bacterial photosynthetic units consist of supra-molecular arrays of peripheral (LH2) and core (LH1-RC) antenna complexes. Recent atomic force microscopy pictures of photosynthetic units in intact membranes have revealed that the architecture of these units is variable (Scheuring et al. (2005) Biochim Bhiophys Acta 1712:109-127). In this study, we describe methods for the construction of heterologous photosynthetic units in lipid-bilayers from mixtures of purified LH2 (from Rhodopseudomonas acidophila) and LH1-RC (from Rhodopseudomonas viridis) core complexes. The architecture of these reconstituted photosynthetic units can be varied by controlling ratio of added LH2 to core complexes. The arrangement of the complexes was visualized by electron-microscopy in combination with Fourier analysis. The regular trigonal array of the core complexes seen in the native photosynthetic membrane could be regenerated in the reconstituted membranes by temperature cycling. In the presence of added LH2 complexes, this trigonal symmetry was replaced with orthorhombic symmetry. The small lattice lengths for the latter suggest that the constituent unit of the orthorhombic lattice is the LH2. Fluorescence and fluorescence-excitation spectroscopy was applied to the set of the reconstituted membranes prepared with various proportions of LH2 to core complexes. Remarkably, even though the LH2 complexes contain bacteriochlorophyll a, and the core complexes contain bacteriochlorophyll b, it was possible to demonstrate energy transfer from LH2 to the core complexes. These experiments provide a first step along the path toward investigating how changing the architecture of purple bacterial photosynthetic units affects the overall efficiency of light-harvesting.

Flowing gas, non-nuclear experiments on the gas core reactor

NASA Technical Reports Server (NTRS)

Kunze, J. F.; Suckling, D. H.; Copper, C. G.

1972-01-01

Flow tests were conducted on models of the gas core (cavity) reactor. Variations in cavity wall and injection configurations were aimed at establishing flow patterns that give a maximum of the nuclear criticality eigenvalue. Correlation with the nuclear effect was made using multigroup diffusion theory normalized by previous benchmark critical experiments. Air was used to simulate the hydrogen propellant in the flow tests, and smoked air, argon, or freon to simulate the central nuclear fuel gas. All tests were run in the down-firing direction so that gravitational effects simulated the acceleration effect of a rocket. Results show that acceptable flow patterns with high volume fraction for the simulated nuclear fuel gas and high flow rate ratios of propellant to fuel can be obtained. Using a point injector for the fuel, good flow patterns are obtained by directing the outer gas at high velocity along the cavity wall, using louvered or oblique-angle-honeycomb injection schemes.

Influence of flow constraints on the properties of the critical endpoint of symmetric nuclear matter

NASA Astrophysics Data System (ADS)

Ivanytskyi, A. I.; Bugaev, K. A.; Sagun, V. V.; Bravina, L. V.; Zabrodin, E. E.

2018-06-01

We propose a novel family of equations of state for symmetric nuclear matter based on the induced surface tension concept for the hard-core repulsion. It is shown that having only four adjustable parameters the suggested equations of state can, simultaneously, reproduce not only the main properties of the nuclear matter ground state, but the proton flow constraint up its maximal particle number densities. Varying the model parameters we carefully examine the range of values of incompressibility constant of normal nuclear matter and its critical temperature, which are consistent with the proton flow constraint. This analysis allows us to show that the physically most justified value of nuclear matter critical temperature is 15.5-18 MeV, the incompressibility constant is 270-315 MeV and the hard-core radius of nucleons is less than 0.4 fm.

Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes*

PubMed Central

Gupta, Kushol; Martin, Renee; Sharp, Robert; Sarachan, Kathryn L.; Ninan, Nisha S.; Van Duyne, Gregory D.

2015-01-01

The survival motor neuron (SMN) protein forms the oligomeric core of a multiprotein complex required for the assembly of spliceosomal small nuclear ribonucleoproteins. Deletions and mutations in the SMN1 gene are associated with spinal muscular atrophy (SMA), a devastating neurodegenerative disease that is the leading heritable cause of infant mortality. Oligomerization of SMN is required for its function, and some SMA patient mutations disrupt the ability of SMN to self-associate. Here, we investigate the oligomeric nature of the SMN·Gemin2 complexes from humans and fission yeast (hSMN·Gemin2 and ySMN·Gemin2). We find that hSMN·Gemin2 forms oligomers spanning the dimer to octamer range. The YG box oligomerization domain of SMN is both necessary and sufficient to form these oligomers. ySMN·Gemin2 exists as a dimer-tetramer equilibrium with Kd = 1.0 ± 0.9 μm. A 1.9 Å crystal structure of the ySMN YG box confirms a high level of structural conservation with the human ortholog in this important region of SMN. Disulfide cross-linking experiments indicate that SMN tetramers are formed by self-association of stable, non-dissociating dimers. Thus, SMN tetramers do not form symmetric helical bundles such as those found in glycine zipper transmembrane oligomers. The dimer-tetramer nature of SMN complexes and the dimer of dimers organization of the SMN tetramer provide an important foundation for ongoing studies to understand the mechanism of SMN-assisted small nuclear ribonucleoprotein assembly and the underlying causes of SMA. PMID:26092730

Nuclear safety considerations in the conceptual design of a fast reactor for space electric power and propulsion

NASA Technical Reports Server (NTRS)

Hsieh, T.-M.; Koenig, D. R.

1977-01-01

Some nuclear safety aspects of a 3.2 mWt heat pipe cooled fast reactor with out-of-core thermionic converters are discussed. Safety related characteristics of the design including a thin layer of B4C surrounding the core, the use of heat pipes and BeO reflector assembly, the elimination of fuel element bowing, etc., are highlighted. Potential supercriticality hazards and countermeasures are considered. Impacts of some safety guidelines of space transportation system are also briefly discussed, since the currently developing space shuttle would be used as the primary launch vehicle for the nuclear electric propulsion spacecraft.

Droplet Core Nuclear Rocket (DCNR)

NASA Technical Reports Server (NTRS)

Anghaie, Samim

1991-01-01

The most basic design feature of the droplet core nuclear reactor is to spray liquid uranium into the core in the form of droplets on the order of five to ten microns in size, to bring the reactor to critical conditions. The liquid uranium fuel ejector is driven by hydrogen, and more hydrogen is injected from the side of the reactor to about one and a half meters from the top. High temperature hydrogen is expanded through a nozzle to produce thrust. The hydrogen pressure in the system can be somewhere between 50 and 500 atmospheres; the higher pressure is more desirable. In the lower core region, hydrogen is tangentially injected to serve two purposes: (1) to provide a swirling flow to protect the wall from impingement of hot uranium droplets: (2) to generate a vortex flow that can be used for fuel separation. The reactor is designed to maximize the energy generation in the upper region of the core. The system can result in and Isp of 2000 per second, and a thrust-to-weight ratio of 1.6 for the shielded reactor. The nuclear engine system can reduce the Mars mission duration to less than 200 days. It can reduce the hydrogen consumption by a factor of 2 to 3, which reduces the hydrogen load by about 130 to 150 metric tons.

Convective cooling in a pool-type research reactor

NASA Astrophysics Data System (ADS)

Sipaun, Susan; Usman, Shoaib

2016-01-01

A reactor produces heat arising from fission reactions in the nuclear core. In the Missouri University of Science and Technology research reactor (MSTR), this heat is removed by natural convection where the coolant/moderator is demineralised water. Heat energy is transferred from the core into the coolant, and the heated water eventually evaporates from the open pool surface. A secondary cooling system was installed to actively remove excess heat arising from prolonged reactor operations. The nuclear core consists of uranium silicide aluminium dispersion fuel (U3Si2Al) in the form of rectangular plates. Gaps between the plates allow coolant to pass through and carry away heat. A study was carried out to map out heat flow as well as to predict the system's performance via STAR-CCM+ simulation. The core was approximated as porous media with porosity of 0.7027. The reactor is rated 200kW and total heat density is approximately 1.07+E7 Wm-3. An MSTR model consisting of 20% of MSTR's nuclear core in a third of the reactor pool was developed. At 35% pump capacity, the simulation results for the MSTR model showed that water is drawn out of the pool at a rate 1.28 kg s-1 from the 4" pipe, and predicted pool surface temperature not exceeding 30°C.

Analysis of loss-of-coolant accident for a fast-spectrum lithium-cooled nuclear reactor for space-power applications

NASA Technical Reports Server (NTRS)

Turney, G. E.; Petrik, E. J.; Kieffer, A. W.

1972-01-01

A two-dimensional, transient, heat-transfer analysis was made to determine the temperature response in the core of a conceptual space-power nuclear reactor following a total loss of reactor coolant. With loss of coolant from the reactor, the controlling mode of heat transfer is thermal radiation. In one of the schemes considered for removing decay heat from the core, it was assumed that the 4 pi shield which surrounds the core acts as a constant-temperature sink (temperature, 700 K) for absorption of thermal radiation from the core. Results based on this scheme of heat removal show that melting of fuel in the core is possible only when the emissivity of the heat-radiating surfaces in the core is less than about 0.40. In another scheme for removing the afterheat, the core centerline fuel pin was replaced by a redundant, constant temperature, coolant channel. Based on an emissivity of 0.20 for all material surfaces in the core, the calculated maximum fuel temperature for this scheme of heat removal was 2840 K, or about 90 K less than the melting temperature of the UN fuel.

Reactors as a Source of Antineutrinos: Effects of Fuel Loading and Burnup for Mixed-Oxide Fuels

NASA Astrophysics Data System (ADS)

Bernstein, Adam; Bowden, Nathaniel S.; Erickson, Anna S.

2018-01-01

In a conventional light-water reactor loaded with a range of uranium and plutonium-based fuel mixtures, the variation in antineutrino production over the cycle reflects both the initial core fissile inventory and its evolution. Under an assumption of constant thermal power, we calculate the rate at which antineutrinos are emitted from variously fueled cores, and the evolution of that rate as measured by a representative ton-scale antineutrino detector. We find that antineutrino flux decreases with burnup for low-enriched uranium cores, increases for full mixed-oxide (MOX) cores, and does not appreciably change for cores with a MOX fraction of approximately 75%. Accounting for uncertainties in the fission yields in the emitted antineutrino spectra and the detector response function, we show that the difference in corewide MOX fractions at least as small as 8% can be distinguished using a hypothesis test. The test compares the evolution of the antineutrino rate relative to an initial value over part or all of the cycle. The use of relative rates reduces the sensitivity of the test to an independent thermal power measurement, making the result more robust against possible countermeasures. This rate-only approach also offers the potential advantage of reducing the cost and complexity of the antineutrino detectors used to verify the diversion, compared to methods that depend on the use of the antineutrino spectrum. A possible application is the verification of the disposition of surplus plutonium in nuclear reactors.

Telomere dysfunction and cell survival: Roles for distinct TIN2-containing complexes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Sahn-ho; Davalos, Albert R.; Heo, Seok-Jin

Telomeres are maintained by three DNA binding proteins (TRF1, TRF2 and POT1), and several associated factors. One factor, TIN2, binds TRF1 and TRF2 directly and POT1 indirectly. Along with two other proteins, TPP1 and hRap1, these form a soluble complex that may be the core telomere maintenance complex. It is not clear whether sub-complexes also exist in vivo. We provide evidence for two TIN2 sub-complexes with distinct functions in human cells. We isolated these two TIN2 sub-complexes from nuclear lysates of unperturbed cells and cells expressing TIN2 mutants TIN2-13, TIN2-15C, which cannot bind TRF2 or TRF1, respectively. In cells withmore » wild-type p53 function, TIN2-15C was more potent than TIN2-13 in causing telomere uncapping and eventual growth arrest. In cells lacking p53 function, TIN2-15C was more potent than TIN2-13 in causing telomere dysfunction and cell death. Our findings suggest that distinct TIN2 complexes exist, and that TIN2-15C-sensitive subcomplexes are particularly important for cell survival in the absence of functional p53.« less

10 CFR 50.46 - Acceptance criteria for emergency core cooling systems for light-water nuclear power reactors.

Code of Federal Regulations, 2011 CFR

2011-01-01

... light-water nuclear power reactors. 50.46 Section 50.46 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC... reactors. (a)(1)(i) Each boiling or pressurized light-water nuclear power reactor fueled with uranium oxide... behavior of the reactor system during a loss-of-coolant accident. Comparisons to applicable experimental...

Hybrid Reduced Order Modeling Algorithms for Reactor Physics Calculations

NASA Astrophysics Data System (ADS)

Bang, Youngsuk

Reduced order modeling (ROM) has been recognized as an indispensable approach when the engineering analysis requires many executions of high fidelity simulation codes. Examples of such engineering analyses in nuclear reactor core calculations, representing the focus of this dissertation, include the functionalization of the homogenized few-group cross-sections in terms of the various core conditions, e.g. burn-up, fuel enrichment, temperature, etc. This is done via assembly calculations which are executed many times to generate the required functionalization for use in the downstream core calculations. Other examples are sensitivity analysis used to determine important core attribute variations due to input parameter variations, and uncertainty quantification employed to estimate core attribute uncertainties originating from input parameter uncertainties. ROM constructs a surrogate model with quantifiable accuracy which can replace the original code for subsequent engineering analysis calculations. This is achieved by reducing the effective dimensionality of the input parameter, the state variable, or the output response spaces, by projection onto the so-called active subspaces. Confining the variations to the active subspace allows one to construct an ROM model of reduced complexity which can be solved more efficiently. This dissertation introduces a new algorithm to render reduction with the reduction errors bounded based on a user-defined error tolerance which represents the main challenge of existing ROM techniques. Bounding the error is the key to ensuring that the constructed ROM models are robust for all possible applications. Providing such error bounds represents one of the algorithmic contributions of this dissertation to the ROM state-of-the-art. Recognizing that ROM techniques have been developed to render reduction at different levels, e.g. the input parameter space, the state space, and the response space, this dissertation offers a set of novel hybrid ROM algorithms which can be readily integrated into existing methods and offer higher computational efficiency and defendable accuracy of the reduced models. For example, the snapshots ROM algorithm is hybridized with the range finding algorithm to render reduction in the state space, e.g. the flux in reactor calculations. In another implementation, the perturbation theory used to calculate first order derivatives of responses with respect to parameters is hybridized with a forward sensitivity analysis approach to render reduction in the parameter space. Reduction at the state and parameter spaces can be combined to render further reduction at the interface between different physics codes in a multi-physics model with the accuracy quantified in a similar manner to the single physics case. Although the proposed algorithms are generic in nature, we focus here on radiation transport models used in support of the design and analysis of nuclear reactor cores. In particular, we focus on replacing the traditional assembly calculations by ROM models to facilitate the generation of homogenized cross-sections for downstream core calculations. The implication is that assembly calculations could be done instantaneously therefore precluding the need for the expensive evaluation of the few-group cross-sections for all possible core conditions. Given the generic natures of the algorithms, we make an effort to introduce the material in a general form to allow non-nuclear engineers to benefit from this work.

10 CFR Appendix A to Part 50 - General Design Criteria for Nuclear Power Plants

Code of Federal Regulations, 2012 CFR

2012-01-01

... Heat Removal 34 Emergency Core Cooling 35 Inspection of Emergency Core Cooling System 36 Testing of Emergency Core Cooling System 37 Containment Heat Removal 38 Inspection of Containment Heat Removal System 39 Testing of Containment Heat Removal System 40 Containment Atmosphere Cleanup 41 Inspection of...

10 CFR Appendix A to Part 50 - General Design Criteria for Nuclear Power Plants

Code of Federal Regulations, 2011 CFR

2011-01-01

... Heat Removal 34 Emergency Core Cooling 35 Inspection of Emergency Core Cooling System 36 Testing of Emergency Core Cooling System 37 Containment Heat Removal 38 Inspection of Containment Heat Removal System 39 Testing of Containment Heat Removal System 40 Containment Atmosphere Cleanup 41 Inspection of...

Identification of a major polypeptide of the nuclear pore complex

PubMed Central

1982-01-01

The nuclear pore complex is a prominent structural component of the nuclear envelope that appears to regulate nucleoplasmic molecular movement. Up to now, none of its polypeptides have been defined. To identify possible pore complex proteins, we fractionated rat liver nuclear envelopes and microsomal membranes with strong protein perturbants into peripheral and intrinsic membrane proteins, and compared these fractions on SDS gels. From this analysis, we identified a prominent 190-kilodalton intrinsic membrane polypeptide that occurs specifically in nuclear envelopes. Lectin binding studies indicate that this polypeptide (gp 190) is the major nuclear envelope glycoprotein. Upon treatment of nuclear envelopes with Triton X-100, gp 190 remains associated with a protein substructure of the nuclear envelope consisting of pore complexes and nuclear lamina. We prepared monospecific antibodies to gp 190 for immunocytochemical localization. Immunofluorescence staining of tissue culture cells suggests that gp 190 occurs exclusively in the nucleus during interphase. This polypeptide becomes dispersed throughout the cell in mitotic prophase when the nuclear envelope is disassembled, and subsequently returns to the nuclear surfaces during telophase when the nuclear envelope is reconstructed. Immunoferritin labeling of Triton-treated rat liver nuclei demonstrates that gp 190 occurs exclusively in the nuclear pore complex, in the regions of the cytoplasmic (and possibly nucleoplasmic) pore complex annuli. A polypeptide that cross-reacts with gp 190 is present in diverse vertebrate species, as shown by antibody labeling of nitrocellulose SDS gel transfers. On the basis of its biochemical characteristics, we suggest that gp 190 may be involved in anchoring the pore complex to nuclear envelope membranes. PMID:7153248

The effect of peculiar complex core balance training on isokinetic muscle functions of the knee and lumbus

PubMed Central

Lee, Myungsun; Han, Gunsoo

2016-01-01

[Purpose] This study aimed to investigate the effect of peculiar complex core balance training on the isokinetic muscle function of the knee joint and lumbus to provide fundamental data for establishing a training program that focuses on improving the performance and prevention of injury by developing the core and low extremity muscles. [Subjects and Methods] The participants in this study included a total of ten high school athletes involved in a throwing event for over five years. The subjects were randomly divided into two groups: The experimental group (N=5) and the control group (N=5). The experimental group underwent peculiar complex core balance training. [Results] According to the analysis of covariance, there was a significant effect of peculiar complex core balance training. Therefore, the isokinetic muscle function of the knee joint and lumbus in the experimental group participating in peculiar complex core balance training was significantly increased compared to the control group. [Conclusion] It is concluded that peculiar complex core balance training had a positive effect on the isokinetic muscle function of the knee and lumbus in throwing event athletes. PMID:27190470

RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination.

PubMed

Snedden, Donald D; Bertke, Michelle M; Vernon, Dominic; Huber, Paul W

2013-07-01

The 3' untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transported to the opposite, animal, hemisphere. A set of proteins that cross-link to the localization elements of vegetally localized RNAs are also cross-linked to PHAX and An1 mRNAs, demonstrating that the composition of RNP complexes that form on these localization elements is highly conserved irrespective of the final destination of the RNA. The ability of RNAs to bind this core group of proteins is correlated with localization activity. Staufen1, which binds to Vg1 and VegT mRNAs, is not associated with RNAs localized to the animal hemisphere and may determine, at least in part, the direction of RNA movement in Xenopus oocytes.

Los Alamos, Toshiba probing Fukushima with cosmic rays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morris, Christopher

2014-06-16

Los Alamos National Laboratory has announced an impending partnership with Toshiba Corporation to use a Los Alamos technique called muon tomography to safely peer inside the cores of the Fukushima Daiichi reactors and create high-resolution images of the damaged nuclear material inside without ever breaching the cores themselves. The initiative could reduce the time required to clean up the disabled complex by at least a decade and greatly reduce radiation exposure to personnel working at the plant. Muon radiography (also called cosmic-ray radiography) uses secondary particles generated when cosmic rays collide with upper regions of Earth's atmosphere to create imagesmore » of the objects that the particles, called muons, penetrate. The process is analogous to an X-ray image, except muons are produced naturally and do not damage the materials they contact. Muon radiography has been used before in imaginative applications such as mapping the interior of the Great Pyramid at Giza, but Los Alamos's muon tomography technique represents a vast improvement over earlier technology.« less

Density matrix-based time-dependent configuration interaction approach to ultrafast spin-flip dynamics

NASA Astrophysics Data System (ADS)

Wang, Huihui; Bokarev, Sergey I.; Aziz, Saadullah G.; Kühn, Oliver

2017-08-01

Recent developments in attosecond spectroscopy yield access to the correlated motion of electrons on their intrinsic timescales. Spin-flip dynamics is usually considered in the context of valence electronic states, where spin-orbit coupling is weak and processes related to the electron spin are usually driven by nuclear motion. However, for core-excited states, where the core-hole has a nonzero angular momentum, spin-orbit coupling is strong enough to drive spin-flips on a much shorter timescale. Using density matrix-based time-dependent restricted active space configuration interaction including spin-orbit coupling, we address an unprecedentedly short spin-crossover for the example of L-edge (2p→3d) excited states of a prototypical Fe(II) complex. This process occurs on a timescale, which is faster than that of Auger decay (∼4 fs) treated here explicitly. Modest variations of carrier frequency and pulse duration can lead to substantial changes in the spin-state yield, suggesting its control by soft X-ray light.

Los Alamos, Toshiba probing Fukushima with cosmic rays

ScienceCinema

Morris, Christopher

2018-01-16

Los Alamos National Laboratory has announced an impending partnership with Toshiba Corporation to use a Los Alamos technique called muon tomography to safely peer inside the cores of the Fukushima Daiichi reactors and create high-resolution images of the damaged nuclear material inside without ever breaching the cores themselves. The initiative could reduce the time required to clean up the disabled complex by at least a decade and greatly reduce radiation exposure to personnel working at the plant. Muon radiography (also called cosmic-ray radiography) uses secondary particles generated when cosmic rays collide with upper regions of Earth's atmosphere to create images of the objects that the particles, called muons, penetrate. The process is analogous to an X-ray image, except muons are produced naturally and do not damage the materials they contact. Muon radiography has been used before in imaginative applications such as mapping the interior of the Great Pyramid at Giza, but Los Alamos's muon tomography technique represents a vast improvement over earlier technology.

Emergency deployable core catcher

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rosewell, M.P.

An emergency melt down core catcher apparatus for a nuclear reactor having a retrofitable eutectic solute holding vessel connected to a core containment vessel with particle transferring fluid and particles or granules of solid eutectic solute materials contained therein and transferable by automatically operated valve means to transport and position the solid eutectic solute material in a position below the core to catch and react with any partial or complete melt down of the fuel core.

Seed and blanket fuel arrangement for dual-phase nuclear reactors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Congdon, S.P.; Fawcett, R.M.

1992-09-22

This patent describes a fuel management method for a dual-phase nuclear reactor, it comprises: installing a fuel bundle at a first core location accessed by coolant through a relatively small aperture, each of the bundles having a predetermined group of fuel elements; operating the reactor a first time; shutting down the reactor; reinstalling the fuel bundle at a second core location accessed by coolant through a relatively large aperture; and operating the reactor a second time.

Comparative efficacy of ways of long-term usage of start-up boilers in the scheme of nuclear power plant

NASA Astrophysics Data System (ADS)

Khroustalev, V. A.; Simonyan, A. A.

2017-11-01

There was carried out an analysis of technical characteristics of boiler houses in a number of Russian NPPs. We justified the possibility of their usage for autonomous generation of electrical energy and improvement of maneuvering properties of power complexes as a single object of regulation, as well as the possibility of increasing the total generation capacity of NPP power units during peak hours. Then the selection of the main equipment of house boiler for its autonomous work was done. There were composed basic thermal diagrams of the power complex on the basis of NPP start-up boiler (SUB) and the satellite turbine. The article also considers some options of reconstruction of SUB into the heat-recovery boiler. The developed power complexes are designed to be used on the basis of the two-loop NPP with pressurized power reactors (PWR). They can be applied with serial and projected domestic NPP units with the aim of getting more power, improving the plant capacity factor (PCF), as well as with the aim of NPP participation in the regulation of the load curve above the nominal value with partial replacement of new construction. The power complexes can be a relevant solution in the light of the energy strategy of the Russian Federation, which is aimed at, firstly, further improvement of efficiency and safety at the NPP, and, secondly, solving the problem of adequate maneuverability and ensuring the adjustment range limits in power systems with high share of nuclear power plants. Implementation of new hybrid thermal diagrams allows simultaneous increase in the safety of NPP, and usage of nuclear power plants emergency frequency control in power systems by fast load drop and rise by -4÷+2 % of the nominal value. Due to the usage of different fuels in power complexes, uranium loading in the core of reactor facilities and gas in SUB, there was proposed and formalized the criterion of “thermoeconomic index”. This criterion represents the ratio of the gross receipt from the sale of electricity to the total cost of fuel of all kinds, spent on ensuring power efficiency.

Core-shell polymer nanoparticles for prevention of GSH drug detoxification and cisplatin delivery to breast cancer cells

NASA Astrophysics Data System (ADS)

Surnar, Bapurao; Sharma, Kavita; Jayakannan, Manickam

2015-10-01

Platinum drug delivery against the detoxification of cytoplasmic thiols is urgently required for achieving efficacy in breast cancer treatment that is over expressed by glutathione (GSH, thiol-oligopeptide). GSH-resistant polymer-cisplatin core-shell nanoparticles were custom designed based on biodegradable carboxylic functional polycaprolactone (PCL)-block-poly(ethylene glycol) diblock copolymers. The core of the nanoparticle was fixed as 100 carboxylic units and the shell part was varied using various molecular weight poly(ethylene glycol) monomethyl ethers (MW of PEGs = 100-5000 g mol-1) as initiator in the ring-opening polymerization. The complexation of cisplatin aquo species with the diblocks produced core-shell nanoparticles of 75 nm core with precise size control the particles up to 190 nm. The core-shell nanoparticles were found to be stable in saline solution and PBS and they exhibited enhanced stability with increase in the PEG shell thickness at the periphery. The hydrophobic PCL layer on the periphery of the cisplatin core behaved as a protecting layer against the cytoplasmic thiol residues (GSH and cysteine) and exhibited <5% of drug detoxification. In vitro drug-release studies revealed that the core-shell nanoparticles were ruptured upon exposure to lysosomal enzymes like esterase at the intracellular compartments. Cytotoxicity studies were performed both in normal wild-type mouse embryonic fibroblast cells (Wt-MEFs), and breast cancer (MCF-7) and cervical cancer (HeLa) cell lines. Free cisplatin and polymer drug core-shell nanoparticles showed similar cytotoxicity effects in the HeLa cells. In MCF-7 cells, the free cisplatin drug exhibited 50% cell death whereas complete cell death (100%) was accomplished by the polymer-cisplatin core-shell nanoparticles. Confocal microscopic images confirmed that the core-shell nanoparticles were taken up by the MCF-7 and HeLa cells and they were accumulated both at the cytoplasm as well at peri-nuclear environments. The present investigation lays a new foundation for the polymer-based core-shell nanoparticles approach for overcoming detoxification in platinum drugs for the treatment of GSH over-expressed breast cancer cells.Platinum drug delivery against the detoxification of cytoplasmic thiols is urgently required for achieving efficacy in breast cancer treatment that is over expressed by glutathione (GSH, thiol-oligopeptide). GSH-resistant polymer-cisplatin core-shell nanoparticles were custom designed based on biodegradable carboxylic functional polycaprolactone (PCL)-block-poly(ethylene glycol) diblock copolymers. The core of the nanoparticle was fixed as 100 carboxylic units and the shell part was varied using various molecular weight poly(ethylene glycol) monomethyl ethers (MW of PEGs = 100-5000 g mol-1) as initiator in the ring-opening polymerization. The complexation of cisplatin aquo species with the diblocks produced core-shell nanoparticles of 75 nm core with precise size control the particles up to 190 nm. The core-shell nanoparticles were found to be stable in saline solution and PBS and they exhibited enhanced stability with increase in the PEG shell thickness at the periphery. The hydrophobic PCL layer on the periphery of the cisplatin core behaved as a protecting layer against the cytoplasmic thiol residues (GSH and cysteine) and exhibited <5% of drug detoxification. In vitro drug-release studies revealed that the core-shell nanoparticles were ruptured upon exposure to lysosomal enzymes like esterase at the intracellular compartments. Cytotoxicity studies were performed both in normal wild-type mouse embryonic fibroblast cells (Wt-MEFs), and breast cancer (MCF-7) and cervical cancer (HeLa) cell lines. Free cisplatin and polymer drug core-shell nanoparticles showed similar cytotoxicity effects in the HeLa cells. In MCF-7 cells, the free cisplatin drug exhibited 50% cell death whereas complete cell death (100%) was accomplished by the polymer-cisplatin core-shell nanoparticles. Confocal microscopic images confirmed that the core-shell nanoparticles were taken up by the MCF-7 and HeLa cells and they were accumulated both at the cytoplasm as well at peri-nuclear environments. The present investigation lays a new foundation for the polymer-based core-shell nanoparticles approach for overcoming detoxification in platinum drugs for the treatment of GSH over-expressed breast cancer cells. Electronic supplementary information (ESI) available: TGA profile and DSC thermogram of all polymers, DLS data, AFM image, 1H-NMR, 13C-NMR, and MALDI spectra of all polymers and monomers. See DOI: 10.1039/c5nr04963f

What is an Oceanic Core Complex?

NASA Astrophysics Data System (ADS)

Schroeder, T.; Cheadle, M. J.

2007-12-01

The Mid-Atlantic Ridge (MAR) 75km north and south of the 15-20 Fracture Zone (FZ) has produced upper oceanic lithosphere composed dominantly of mantle peridotite with gabbro intrusions. In the absence of diapirism, mantle peridotite can only be exposed on the seafloor by extensional faulting, thus the sea floor geology and bathymetry provide widespread evidence for extensive low-angle faulting. However, only 3% of the seafloor in this region has the domal morphology characteristic of features that have been termed oceanic core complexes; suggesting that other processes, in addition to low-angle faulting, are responsible for the generation of domal core complexes. Most low-angle faults near the 15-20 FZ form gently dipping (10-15°), 10-15km-wide dip slopes on the flanks of 2000m relief bathymetric ridges that are up to 15-40km long (parallel to the MAR). Core recovered from ODP Leg 209 drill holes in these ridges is dominantly peridotite with small (<50m thick) gabbro intrusions. The peridotite is cut by a very high density of brittle faults dipping at both steep and gentle angles. Several holes also contain long-lived shear zones/faults in their upper reaches in which strain was localized at granulite facies, indicated by mylonitic olivine and cpx, and remained active during cooling to sub-greenschist grade, indicated by cross-cutting of progressively lower-grade syn-deformation mineral assemblages. These observations suggest that seafloor spreading is largely accommodated here by slip on low-angle faults, and that these faults are correctly termed detachment faults. Holes drilled into a domal oceanic core complex north of the 15-20 FZ during Leg 209 (ODP Site 1275) recovered dominantly gabbro and not mantle peridotite. This hole is cut by significantly fewer brittle and ductile faults than the peridotite drilled at the non-core-complex detachment fault sites. The detachment fault in the upper reaches (50m) of Site 1275 was localized at temperatures near feldspar's ductile-to-brittle transition, indicated by cataclasis with minor crystal plastic flow in plagioclase, and a lack of pervasive pure-ductile deformation. Amphibole-plagioclase thermometry in the fault yields equilibrium temperatures from 600-650°C, compared to equilibrium temperatures of 750-850°C for the gabbro outside the fault. The presence of talc- chlorite schists and cataclasites cutting the higher-temperature deformation textures indicate fault activity down- temperature from amphibolite through greenschist facies. This core-complex-bounding fault contrasts with the fault that bounds the Atlantis Bank Core Complex on the Southwest Indian Ridge (SWIR). There, the fault is 100m thick and strain was initially localized at granulite grade (>800°C) (Mehl & Hirth, 2007); significantly hotter than the Site 1275 fault. Therefore, the formation of core-complex morphology does not seem to depend on the initial faulting conditions. Both oceanic core complexes that have been drilled besides Site 1275, Atlantis Massif at 30°N (IODP Hole 1309D) on the MAR and Atlantis Bank on the SWIR (ODP Hole 735B), are also comprised dominantly of gabbro. This suggests that magma supply may be an essential requirement for core complex formation and raises the question whether all domal oceanic core complexes are cored by gabbro? We also ask whether the term 'oceanic core complex' should be restricted to these domal features and not applied to detachment-bound, non- domal, peridotite-cored ridges; or if these should be considered two sub-classes of oceanic core complexes.

Proteasome dynamics between proliferation and quiescence stages of Saccharomyces cerevisiae.

PubMed

Yedidi, Ravikiran S; Fatehi, Amatullah K; Enenkel, Cordula

The ubiquitin-proteasome system (UPS) plays a critical role in cellular protein homeostasis and is required for the turnover of short-lived and unwanted proteins, which are targeted by poly-ubiquitination for degradation. Proteasome is the key protease of UPS and consists of multiple subunits, which are organized into a catalytic core particle (CP) and a regulatory particle (RP). In Saccharomyces cerevisiae, proteasome holo-enzymes are engaged in degrading poly-ubiquitinated substrates and are mostly localized in the nucleus during cell proliferation. While in quiescence, the RP and CP are sequestered into motile and reversible storage granules in the cytoplasm, called proteasome storage granules (PSGs). The reversible nature of PSGs allows the proteasomes to be transported back into the nucleus upon exit from quiescence. Nuclear import of RP and CP through nuclear pores occurs via the canonical pathway that includes the importin-αβ heterodimer and takes advantage of the Ran-GTP gradient across the nuclear membrane. Dependent on the growth stage, either inactive precursor complexes or mature holo-enzymes are imported into the nucleus. The present review discusses the dynamics of proteasomes including their assembly, nucleo-cytoplasmic transport during proliferation and the sequestration of proteasomes into PSGs during quiescence. [Formula: see text].

Abnormal XPD-induced nuclear receptor transactivation in DNA repair disorders: trichothiodystrophy and xeroderma pigmentosum.

PubMed

Zhou, Xiaolong; Khan, Sikandar G; Tamura, Deborah; Ueda, Takahiro; Boyle, Jennifer; Compe, Emmanuel; Egly, Jean-Marc; DiGiovanna, John J; Kraemer, Kenneth H

2013-08-01

XPD (ERCC2) is a DNA helicase involved in nucleotide excision repair and in transcription as a structural bridge tying the transcription factor IIH (TFIIH) core with the cdk-activating kinase complex, which phosphorylates nuclear receptors. Mutations in XPD are associated with several different phenotypes, including trichothiodystrophy (TTD), with sulfur-deficient brittle hair, bone defects, and developmental abnormalities without skin cancer, xeroderma pigmentosum (XP), with pigmentary abnormalities and increased skin cancer, or XP/TTD with combined features, including skin cancer. We describe the varied clinical features and mutations in nine patients examined at the National Institutes of Health who were compound heterozygotes for XPD mutations but had different clinical phenotypes: four TTD, three XP, and two combined XP/TTD. We studied TFIIH-dependent transactivation by nuclear receptor for vitamin D (VDR) and thyroid in cells from these patients. The vitamin D stimulation ratio of CYP24 and osteopontin was associated with specific pairs of mutations (reduced in 5, elevated in 1) but not correlated with distinct clinical phenotypes. Thyroid receptor stimulation ratio for KLF9 was not significantly different from normal. XPD mutations frequently were associated with abnormal VDR stimulation in compound heterozygote patients with TTD, XP, or XP/TTD.

The RNA Export Factor, Nxt1, Is Required for Tissue Specific Transcriptional Regulation

PubMed Central

Jiang, Jianqiao; White-Cooper, Helen

2013-01-01

The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this gene in tissue specific transcription. We show that many Drosophila melanogaster testis-specific mRNAs require Nxt1 for their accumulation. The transcripts that require Nxt1 also depend on a testis-specific transcription complex, tMAC. We show that loss of Nxt1 leads to reduced transcription of tMAC targets. A reporter transcript from a tMAC-dependent promoter is under-expressed in Nxt1 mutants, however the same transcript accumulates in mutants if driven by a tMAC-independent promoter. Thus, in Drosophila primary spermatocytes, the transcription factor used to activate expression of a transcript, rather than the RNA sequence itself or the core transcription machinery, determines whether this expression requires Nxt1. We additionally find that transcripts from intron-less genes are more sensitive to loss of Nxt1 function than those from intron-containing genes and propose a mechanism in which transcript processing feeds back to increase activity of a tissue specific transcription complex. PMID:23754955

A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs.

PubMed

Gromadzka, Agnieszka M; Steckelberg, Anna-Lena; Singh, Kusum K; Hofmann, Kay; Gehring, Niels H

2016-03-18

The export of messenger RNAs (mRNAs) is the final of several nuclear posttranscriptional steps of gene expression. The formation of export-competent mRNPs involves the recruitment of export factors that are assumed to facilitate transport of the mature mRNAs. Using in vitro splicing assays, we show that a core set of export factors, including ALYREF, UAP56 and DDX39, readily associate with the spliced RNAs in an EJC (exon junction complex)- and cap-dependent manner. In order to elucidate how ALYREF and other export adaptors mediate mRNA export, we conducted a computational analysis and discovered four short, conserved, linear motifs present in RNA-binding proteins. We show that mutation in one of the new motifs (WxHD) in an unstructured region of ALYREF reduced RNA binding and abolished the interaction with eIF4A3 and CBP80. Additionally, the mutation impaired proper localization to nuclear speckles and export of a spliced reporter mRNA. Our results reveal important details of the orchestrated recruitment of export factors during the formation of export competent mRNPs. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

A new approach to nuclear fuel safeguard enhancement through radionuclide profiling

NASA Astrophysics Data System (ADS)

Peterson, Aaron Dawon

The United States has led the effort to promote peaceful use of nuclear power amongst states actively utilizing it as well as those looking to deploy the technology in the near future. With the attraction being demonstrated by various countries towards nuclear power comes the concern that a nation may have military aspirations for the use of nuclear energy. The International Atomic Energy Agency (IAEA) has established nuclear safeguard protocols and procedures to mitigate nuclear proliferation. The work herein proposed a strategy to further enhance existing safeguard protocols by considering safeguard in nuclear fuel design. The strategy involved the use of radionuclides to profile nuclear fuels. Six radionuclides were selected as identifier materials. The decay and transmutation of these radionuclides were analyzed in reactor operation environment. MCNPX was used to simulate a reactor core. The perturbation in reactivity of the core due to the loading of the radionuclides was insignificant. The maximum positive and negative reactivity change induced was at day 1900 with a value of 0.00185 +/- 0.00256 and at day 2000 with -0.00441 +/- 0.00249, respectively. The mass of the radionuclides were practically unaffected by transmutation in the core; the change in radionuclide inventory was dominated by natural decay. The maximum material lost due to transmutation was 1.17% in Eu154. Extraneous signals from fission products identical to the radionuclide compromised the identifier signals. Eu154 saw a maximum intensity change at EOC and 30 days post-irradiation of 1260% and 4545%, respectively. Cs137 saw a minimum change of 12% and 89%, respectively. Mitigation of the extraneous signals is cardinal to the success of the proposed strategy. The predictability of natural decay provides a basis for the characterization of the signals from the radionuclide.

A 60-year record of 129I in Taal Lake sediments (Philippines): Influence of human nuclear activities at low latitude regions.

PubMed

Zhang, Luyuan; Hou, Xiaolin; Li, Hong-Chun; Xu, Xiaomei

2018-02-01

The influence of human nuclear activities on environmental radioactivity is not well known at low latitude regions that are distant from nuclear test sites and nuclear facilities. A sediment core collected from Taal Lake in the central Philippines was analyzed for 129 I and 127 I to investigate this influence in a low-latitude terrestrial system. A baseline of 129 I/ 127 I atomic ratios was established at (2.04-5.14) × 10 -12 in the pre-nuclear era in this region. Controlled by the northeasterly equatorial trade winds, increased 129 I/ 127 I ratios of (20.1-69.3) × 10 -12 suggest that atmospheric nuclear weapons tests at the Pacific Proving Grounds in the central Pacific Ocean was the major source of 129 I in the sediment during 1956-1962. The 129 I/ 127 I ratios, up to 157.5 × 10 -12 after 1964, indicate a strong influence by European nuclear fuel reprocessing plants. The East Asian Winter Monsoon is found to be the dominant driving force in the atmospheric dispersion of radioactive iodine ( 129 I) from the European nuclear fuel reprocessing plants to Southeast Asia, which is also important for dispersion of other airborne pollutants from the middle-high to low latitude regions. A significant 129 I/ 127 I peak at 42.8 cm in the Taal Lake core appears to be the signal of the Chernobyl accident in 1986. In addition, volcanic activities are reflected in the iodine isotope profiles in the sediment core, suggesting the potential of using iodine isotopes as an indicator of volcanic eruptions. Copyright © 2017 Elsevier Ltd. All rights reserved.

Light clusters in nuclear matter: Excluded volume versus quantum many-body approaches

NASA Astrophysics Data System (ADS)

Hempel, Matthias; Schaffner-Bielich, Jürgen; Typel, Stefan; Röpke, Gerd

2011-11-01

The formation of clusters in nuclear matter is investigated, which occurs, e.g., in low-energy heavy-ion collisions or core-collapse supernovae. In astrophysical applications, the excluded volume concept is commonly used for the description of light clusters. Here we compare a phenomenological excluded volume approach to two quantum many-body models, the quantum statistical model and the generalized relativistic mean-field model. All three models contain bound states of nuclei with mass number A≤4. It is explored to which extent the complex medium effects can be mimicked by the simpler excluded volume model, regarding the chemical composition and thermodynamic variables. Furthermore, the role of heavy nuclei and excited states is investigated by use of the excluded volume model. At temperatures of a few MeV the excluded volume model gives a poor description of the medium effects on the light clusters, but there the composition is actually dominated by heavy nuclei. At larger temperatures there is a rather good agreement, whereas some smaller differences and model dependencies remain.

Idaho National Laboratory 2015-2023 Ten-Year Site Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sheryl Morton; Elizabeth Connell; Bill Buyers

2013-09-01

This Idaho National Laboratory (INL) Ten-Year Site Plan (TYSP) describes the strategy for accomplishing the long-term objective of sustaining the INL infrastructure to meet the Department of Energy Office of Nuclear Energy (DOE-NE) mission: to promote nuclear power as a resource capable of making major contributions in meeting the nation’s energy supply, environmental and energy security needs. This TYSP provides the strategy for INL to accomplish its mission by: (1) linking R&D mission goals to core capabilities and infrastructure requirements; (2) establishing a ten-year end-state vision for INL facility complexes; (3) identifying and prioritizing infrastructure needs and capability gaps; (4)more » establishing maintenance and repair strategies that allow for sustainment of mission-critical (MC) facilities; and (5) applying sustainability principles to each decision and action. The TYSP serves as the infrastructure-planning baseline for INL; and, though budget formulation documents are informed by the TYSP, it is not itself a budget document.« less

Thermionic system evaluated test (TSET) facility description

NASA Astrophysics Data System (ADS)

Fairchild, Jerry F.; Koonmen, James P.; Thome, Frank V.

1992-01-01

A consortium of US agencies are involved in the Thermionic System Evaluation Test (TSET) which is being supported by the Strategic Defense Initiative Organization (SDIO). The project is a ground test of an unfueled Soviet TOPAZ-II in-core thermionic space reactor powered by electrical heat. It is part of the United States' national thermionic space nuclear power program. It will be tested in Albuquerque, New Mexico at the New Mexico Engineering Research Institute complex by the Phillips Laboratoty, Sandia National Laboratories, Los Alamos National Laboratory, and the University of New Mexico. One of TSET's many objectives is to demonstrate that the US can operate and test a complete space nuclear power system, in the electrical heater configuration, at a low cost. Great efforts have been made to help reduce facility costs during the first phase of this project. These costs include structural, mechanical, and electrical modifications to the existing facility as well as the installation of additional emergency systems to mitigate the effects of utility power losses and alkali metal fires.

The Aviation Paradox: Why We Can 'Know' Jetliners But Not Reactors.

PubMed

Downer, John

2017-01-01

Publics and policymakers increasingly have to contend with the risks of complex, safety-critical technologies, such as airframes and reactors. As such, 'technological risk' has become an important object of modern governance, with state regulators as core agents, and 'reliability assessment' as the most essential metric. The Science and Technology Studies (STS) literature casts doubt on whether or not we should place our faith in these assessments because predictively calculating the ultra-high reliability required of such systems poses seemingly insurmountable epistemological problems. This paper argues that these misgivings are warranted in the nuclear sphere, despite evidence from the aviation sphere suggesting that such calculations can be accurate. It explains why regulatory calculations that predict the reliability of new airframes cannot work in principle, and then it explains why those calculations work in practice. It then builds on this explanation to argue that the means by which engineers manage reliability in aviation is highly domain-specific, and to suggest how a more nuanced understanding of jetliners could inform debates about nuclear energy.

NUCLEAR REACTOR CORE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bell, F.R.

1963-02-01

A nuclear reactor core composed of a number of identical elements of solid moderator material fitted together was designed. Each moderator element is apertured to provide channels for fuel and coolant. The elements have an external shape which permits them to be stacked in layers with similar elements, with the surfaces of adjacent elements fitting and in contact with each other. The cross section of the element is of a general hexagonal shape with identations and protrusions, so that the elements can be fitted together. The described core should not be liable to fracture under transverse loading. Specific arrangements ofmore » moderator elements and fuel and coolant apertures are described. (M.P.G.)« less

Calculation of the neutron diffusion equation by using Homotopy Perturbation Method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koklu, H., E-mail: koklu@gantep.edu.tr; Ozer, O.; Ersoy, A.

The distribution of the neutrons in a nuclear fuel element in the nuclear reactor core can be calculated by the neutron diffusion theory. It is the basic and the simplest approximation for the neutron flux function in the reactor core. In this study, the neutron flux function is obtained by the Homotopy Perturbation Method (HPM) that is a new and convenient method in recent years. One-group time-independent neutron diffusion equation is examined for the most solved geometrical reactor core of spherical, cubic and cylindrical shapes, in the frame of the HPM. It is observed that the HPM produces excellent resultsmore » consistent with the existing literature.« less

Induction simulation of gas core nuclear engine

NASA Technical Reports Server (NTRS)

Poole, J. W.; Vogel, C. E.

1973-01-01

The design, construction and operation of an induction heated plasma device known as a combined principles simulator is discussed. This device incorporates the major design features of the gas core nuclear rocket engine such as solid feed, propellant seeding, propellant injection through the walls, and a transpiration cooled, choked flow nozzle. Both argon and nitrogen were used as propellant simulating material, and sodium was used for fuel simulating material. In addition, a number of experiments were conducted utilizing depleted uranium as the fuel. The test program revealed that satisfactory operation of this device can be accomplished over a range of operating conditions and provided additional data to confirm the validity of the gas core concept.

Laser cutting apparatus for nuclear core fuel subassembly

DOEpatents

Walch, Allan P.; Caruolo, Antonio B.

1982-02-23

The object of the invention is to provide a system and apparatus which employs laser cutting to disassemble a nuclear core fuel subassembly. The apparatus includes a gantry frame (C) which straddles the core fuel subassembly (14), an x-carriage (22) travelling longitudinally above the frame which carries a focus head assembly (D) having a vertically moving carriage (46) and a laterally moving carriage (52), a system of laser beam transferring and focusing mirrors carried by the x-carriage and focusing head assembly, and a shroud follower (F) and longitudinal follower (G) for following the shape of shroud (14) to maintain a beam focal point (44) fixed upon the shroud surface for accurate cutting.

Molecular structure of the pyruvate dehydrogenase complex from Escherichia coli K-12.

PubMed

Vogel, O; Hoehn, B; Henning, U

1972-06-01

The pyruvate dehydrogenase core complex from E. coli K-12, defined as the multienzyme complex that can be obtained with a unique polypeptide chain composition, has a molecular weight of 3.75 x 10(6). All results obtained agree with the following numerology. The core complex consists of 48 polypeptide chains. There are 16 chains (molecular weight = 100,000) of the pyruvate dehydrogenase component, 16 chains (molecular weight = 80,000) of the dihydrolipoamide dehydrogenase component, and 16 chains (molecular weight = 56,000) of the dihydrolipoamide dehydrogenase component. Usually, but not always, pyruvate dehydrogenase complex is produced in vivo containing at least 2-3 mol more of dimers of the pyruvate dehydrogenase component than the stoichiometric ratio with respect to the core complex. This "excess" component is bound differently than are the eight dimers in the core complex.

Trace element fluxes during the last 100 years in sediment near a nuclear power plant

NASA Astrophysics Data System (ADS)

Bojórquez-Sánchez, S.; Marmolejo-Rodríguez, A. J.; Ruiz-Fernández, A. C.; Sánchez-González, A.; Sánchez-Cabeza, J. A.; Bojórquez-Leyva, H.; Pérez-Bernal, L. H.

2017-11-01

The Salada coastal lagoon is located in Veracruz (Mexico) near the Laguna Verde Nuclear Power Plant (LVNPP). Currently, the lagoon receives the cooling waters used in the LVNPP. To evaluate the fluxes and mobilization of trace elements due to human activities in the area, two sediment cores from the coastal flood plains of Salada Lagoon were analysed. Cores were collected using PVC tubes. Sediments cores were analysed every centimetre for dating (210Pb by alpha detector) and trace metal analysis using ICP-Mass Spectrometry. The dating of both sediment cores covers the period from 1900 to 2013, which includes the construction of the LVNPP (1970's). The Normalized Enrichment Factor shows enrichment of Ag, As and Cr in both sediment cores. These enrichments correspond to the extent of mining activity (which reached a maximum in the 1900's) and to the geological setting of the coastal zone. The profiles of the element fluxes in both sediment cores reflected the construction and operation of the LVNPP; however, the elements content did not show evidence of pollution coming from the LVNPP.

Nuclear Pore Complexes: Global Conservation and Local Variation.

PubMed

Holzer, Guillaume; Antonin, Wolfram

2018-06-04

Nuclear pore complexes are the transport gates to the nucleus. Most proteins forming these huge complexes are evolutionarily conserved, as is the eightfold symmetry of these complexes. A new study reporting the structure of the yeast nuclear pore complex now shows striking differences from its human counterpart. Copyright © 2018 Elsevier Ltd. All rights reserved.

United States Air Force Graduate Student Summer Support Program (1987). Program Technical Report. Volume 1.

DTIC Science & Technology

1987-12-01

developed for a large percentage of the participants in the Summer Faculty Research Program in 1979-1983 period through an AFOSR Minigrant Program . On 1...Analysis of a Bimodal Nuclear Rocket Core by Dav,, C. Carpenter ABSTRACT The framework for a general purpose finite element analysis code was developed ...to study the 2-D temperature distribution in a hot-channel S hexagonal fuel element in the core of a bimodal nuclear’ rocket. Prelim- inary thermal

Review of coaxial flow gas core nuclear rocket fluid mechanics

NASA Technical Reports Server (NTRS)

Weinstein, H.

1976-01-01

Almost all of the fluid mechanics research associated with the coaxial flow gas core reactor ended abruptly with the interruption of NASA's space nuclear program because of policy and budgetary considerations in 1973. An overview of program accomplishments is presented through a review of the experiments conducted and the analyses performed. Areas are indicated where additional research is required for a fuller understanding of cavity flow and of the factors which influence cold and hot flow containment. A bibliography is included with graphic material.

Nuclear reactor fuel element

DOEpatents

Johnson, Carl E.; Crouthamel, Carl E.

1980-01-01

A nuclear reactor fuel element is described which has an outer cladding, a central core of fissionable or mixed fissionable and fertile fuel material and a layer of oxygen gettering material on the inner surface of the cladding. The gettering material reacts with oxygen released by the fissionable material during irradiation of the core thereby preventing the oxygen from reacting with and corroding the cladding. Also described is an improved method for coating the inner surface of the cladding with a layer of gettering material.

Muon trackers for imaging a nuclear reactor

NASA Astrophysics Data System (ADS)

Kume, N.; Miyadera, H.; Morris, C. L.; Bacon, J.; Borozdin, K. N.; Durham, J. M.; Fuzita, K.; Guardincerri, E.; Izumi, M.; Nakayama, K.; Saltus, M.; Sugita, T.; Takakura, K.; Yoshioka, K.

2016-09-01

A detector system for assessing damage to the cores of the Fukushima Daiichi nuclear reactors by using cosmic-ray muon tomography was developed. The system consists of a pair of drift-tube tracking detectors of 7.2× 7.2-m2 area. Each muon tracker consists of 6 x-layer and 6 y-layer drift-tube detectors. Each tracker is capable of measuring muon tracks with 12 mrad angular resolutions, and is capable of operating under 50-μ Sv/h radiation environment by removing gamma induced background with a novel time-coincidence logic. An estimated resolution to observe nuclear fuel debris at Fukushima Daiichi is 0.3 m when the core is imaged from outside the reactor building.

Theory of long-range interactions for Rydberg states attached to hyperfine-split cores

NASA Astrophysics Data System (ADS)

Robicheaux, F.; Booth, D. W.; Saffman, M.

2018-02-01

The theory is developed for one- and two-atom interactions when the atom has a Rydberg electron attached to a hyperfine-split core state. This situation is relevant for some of the rare-earth and alkaline-earth atoms that have been proposed for experiments on Rydberg-Rydberg interactions. For the rare-earth atoms, the core electrons can have a very substantial total angular momentum J and a nonzero nuclear spin I . In the alkaline-earth atoms there is a single (s ) core electron whose spin can couple to a nonzero nuclear spin for odd isotopes. The resulting hyperfine splitting of the core state can lead to substantial mixing between the Rydberg series attached to different thresholds. Compared to the unperturbed Rydberg series of the alkali-metal atoms, the series perturbations and near degeneracies from the different parity states could lead to qualitatively different behavior for single-atom Rydberg properties (polarizability, Zeeman mixing and splitting, etc.) as well as Rydberg-Rydberg interactions (C5 and C6 matrices).

Analysis of Radionuclide Releases from the Fukushima Dai-Ichi Nuclear Power Plant Accident Part I

NASA Astrophysics Data System (ADS)

Le Petit, G.; Douysset, G.; Ducros, G.; Gross, P.; Achim, P.; Monfort, M.; Raymond, P.; Pontillon, Y.; Jutier, C.; Blanchard, X.; Taffary, T.; Moulin, C.

2014-03-01

Part I of this publication deals with the analysis of fission product releases consecutive to the Fukushima Dai-ichi accident. Reactor core damages are assessed relying on radionuclide detections performed by the CTBTO radionuclide network, especially at the particulate station located at Takasaki, 210 km away from the nuclear power plant. On the basis of a comparison between the reactor core inventory at the time of reactor shutdowns and the fission product activities measured in air at Takasaki, especially 95Nb and 103Ru, it was possible to show that the reactor cores were exposed to high temperature for a prolonged time. This diagnosis was confirmed by the presence of 113Sn in air at Takasaki. The 133Xe assessed release at the time of reactor shutdown (8 × 1018 Bq) turned out to be in the order of 80 % of the amount deduced from the reactor core inventories. This strongly suggests a broad meltdown of reactor cores.

NUCLEAR REACTOR CORE DESIGN

DOEpatents

Mahlmeister, J.E.; Peck, W.S.; Haberer, W.V.; Williams, A.C.

1960-03-22

An improved core design for a sodium-cooled, graphitemoderated nuclear reactor is described. The improved reactor core comprises a number of blocks of moderator material, each block being in the shape of a regular prism. A number of channels, extending the length of each block, are disposed around the periphery. When several blocks are placed in contact to form the reactor core, the channels in adjacent blocks correspond with each other to form closed conduits extending the length of the core. Fuel element clusters are disposed in these closed conduits, and liquid coolant is forced through the annulus between the fuel cluster and the inner surface of the conduit. In a preferred embodiment of the invention, the moderator blocks are in the form of hexagonal prisms with longitudinal channels cut into the corners of the hexagon. The main advantage of an "edge-loaded" moderator block is that fewer thermal neutrons are absorbed by the moderator cladding, as compared with a conventional centrally loaded moderator block.

Gradual collapse of nuclear wave functions regulated by frequency tuned X-ray scattering.

PubMed

Ignatova, Nina; Cruz, Vinícius V; Couto, Rafael C; Ertan, Emelie; Zimin, Andrey; Guimarães, Freddy F; Polyutov, Sergey; Ågren, Hans; Kimberg, Victor; Odelius, Michael; Gel'mukhanov, Faris

2017-03-07

As is well established, the symmetry breaking by isotope substitution in the water molecule results in localisation of the vibrations along one of the two bonds in the ground state. In this study we find that this localisation may be broken in excited electronic states. Contrary to the ground state, the stretching vibrations of HDO are delocalised in the bound core-excited state in spite of the mass difference between hydrogen and deuterium. The reason for this effect can be traced to the narrow "canyon-like" shape of the potential of the state along the symmetric stretching mode, which dominates over the localisation mass-difference effect. In contrast, the localisation of nuclear motion to one of the HDO bonds is preserved in the dissociative core-excited state . The dynamics of the delocalisation of nuclear motion in these core-excited states is studied using resonant inelastic X-ray scattering of the vibrationally excited HDO molecule. The results shed light on the process of a wave function collapse. After core-excitation into the state of HDO the initial wave packet collapses gradually, rather than instantaneously, to a single vibrational eigenstate.

Connecting Core Percolation and Controllability of Complex Networks

PubMed Central

Jia, Tao; Pósfai, Márton

2014-01-01

Core percolation is a fundamental structural transition in complex networks related to a wide range of important problems. Recent advances have provided us an analytical framework of core percolation in uncorrelated random networks with arbitrary degree distributions. Here we apply the tools in analysis of network controllability. We confirm analytically that the emergence of the bifurcation in control coincides with the formation of the core and the structure of the core determines the control mode of the network. We also derive the analytical expression related to the controllability robustness by extending the deduction in core percolation. These findings help us better understand the interesting interplay between the structural and dynamical properties of complex networks. PMID:24946797

Nuclear pore complex tethers to the cytoskeleton.

PubMed

Goldberg, Martin W

2017-08-01

The nuclear envelope is tethered to the cytoskeleton. The best known attachments of all elements of the cytoskeleton are via the so-called LINC complex. However, the nuclear pore complexes, which mediate the transport of soluble and membrane bound molecules, are also linked to the microtubule network, primarily via motor proteins (dynein and kinesins) which are linked, most importantly, to the cytoplasmic filament protein of the nuclear pore complex, Nup358, by the adaptor BicD2. The evidence for such linkages and possible roles in nuclear migration, cell cycle control, nuclear transport and cell architecture are discussed. Copyright © 2017. Published by Elsevier Ltd.

Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane.

PubMed

Zara, Vincenzo; Conte, Laura; Trumpower, Bernard L

2009-04-01

The assembly status of the cytochrome bc(1) complex has been analyzed in distinct yeast deletion strains in which genes for one or more of the bc(1) subunits were deleted. In all the yeast strains tested, a bc(1) sub-complex of approximately 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis. The subsequent molecular characterization of this sub-complex, carried out in the second dimension by SDS/PAGE and immunodecoration, revealed the presence of the two catalytic subunits, cytochrome b and cytochrome c(1), associated with the noncatalytic subunits core protein 1, core protein 2, Qcr7p and Qcr8p. Together, these bc(1) subunits build up the core structure of the cytochrome bc(1) complex, which is then able to sequentially bind the remaining subunits, such as Qcr6p, Qcr9p, the Rieske iron-sulfur protein and Qcr10p. This bc(1) core structure may represent a true assembly intermediate during the maturation of the bc(1) complex; first, because of its wide distribution in distinct yeast deletion strains and, second, for its characteristics of stability, which resemble those of the intact homodimeric bc(1) complex. By contrast, the bc(1) core structure is unable to interact with the cytochrome c oxidase complex to form respiratory supercomplexes. The characterization of this novel core structure of the bc(1) complex provides a number of new elements clarifying the molecular events leading to the maturation of the yeast cytochrome bc(1) complex in the inner mitochondrial membrane.

Evidence that assembly of the yeast cytochrome bc1 complex involves formation of a large core structure in the inner mitochondrial membrane

PubMed Central

Zara, Vincenzo; Conte, Laura; Trumpower, Bernard L.

2009-01-01

The assembly status of the cytochrome bc1 complex has been analyzed in distinct yeast deletion strains in which genes for one or more of the bc1 subunits had been deleted. In all the yeast strains tested a bc1 sub-complex of about 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis. The subsequent molecular characterization of this sub-complex, carried out in the second dimension by SDS-PAGE and immunodecoration, revealed the presence of the two catalytic subunits cytochrome b and cytochrome c1, associated with the non catalytic subunits core protein 1, core protein 2, Qcr7p and Qcr8p. Altogether these bc1 subunits build up the core structure of the cytochrome bc1 complex which is then able to sequentially bind the remaining subunits, such as Qcr6p, Qcr9p, the Rieske iron-sulfur protein and Qcr10p. This bc1 core structure may represent a true assembly intermediate during the maturation of the bc1 complex, first because of its wide distribution in distinct yeast deletion strains and second for its characteristics of stability which resemble those of the intact homodimeric bc1 complex. Differently from this latter, however, the bc1 core structure is not able to interact with the cytochrome c oxidase complex to form respiratory supercomplexes. The characterization of this novel core structure of the bc1 complex provides a number of new elements for clarification of the molecular events leading to the maturation of the yeast cytochrome bc1 complex in the inner mitochondrial membrane. PMID:19236481

Subparsec-scale structure and evolution of Centaurus A (NGC5128).

PubMed Central

Jauncey, D L; Tingay, S J; Preston, R A; Reynolds, J E; Lovell, J E; McCulloch, P M; Tzioumis, A K; Costa, M E; Murphy, D W; Meier, D L; Jones, D L; Amy, S W; Biggs, J D; Blair, D G; Clay, R W; Edwards, P G; Ellingsen, S P; Ferris, R H; Gough, R G; Harbison, P; Jones, P A; King, E A; Kemball, A J; Migenes, V; Nicolson, G D; Sinclair, M W; Van Ommen, T; Wark, R M; White, G L

1995-01-01

We present a series of 8.4-GHz very-long-baseline radio interferometry images of the nucleus of Centaurus A (NGC5128) made with a Southern Hemisphere array, representing a 3.3-year monitoring effort. The nuclear radio jet is approximately 50 milliarcseconds in extent, or at the 3.5-megaparsec distance of NGC5128, approximately 1 parsec in length. Subluminal motion is seen and structural changes are observed on time scales shorter than 4 months. High-resolution observations at 4.8 and 8.4 GHz made in November 1992 reveal a complex morphology and allow us to unambiguously identify the self-absorbed core located at the southwestern end of the jet. PMID:11607599

Subparsec-scale structure and evolution of Centaurus A (NGC5128).

PubMed

Jauncey, D L; Tingay, S J; Preston, R A; Reynolds, J E; Lovell, J E; McCulloch, P M; Tzioumis, A K; Costa, M E; Murphy, D W; Meier, D L; Jones, D L; Amy, S W; Biggs, J D; Blair, D G; Clay, R W; Edwards, P G; Ellingsen, S P; Ferris, R H; Gough, R G; Harbison, P; Jones, P A; King, E A; Kemball, A J; Migenes, V; Nicolson, G D; Sinclair, M W; Van Ommen, T; Wark, R M; White, G L

1995-12-05

We present a series of 8.4-GHz very-long-baseline radio interferometry images of the nucleus of Centaurus A (NGC5128) made with a Southern Hemisphere array, representing a 3.3-year monitoring effort. The nuclear radio jet is approximately 50 milliarcseconds in extent, or at the 3.5-megaparsec distance of NGC5128, approximately 1 parsec in length. Subluminal motion is seen and structural changes are observed on time scales shorter than 4 months. High-resolution observations at 4.8 and 8.4 GHz made in November 1992 reveal a complex morphology and allow us to unambiguously identify the self-absorbed core located at the southwestern end of the jet.

Activities for Challenging Gifted Learners by Increasing Complexity in the Common Core

ERIC Educational Resources Information Center

McKeone, Alyssa; Caruso, Lenora; Bettle, Kailyn; Chase, Ashley; Bryson, Bridget; Schneider, Jean S.; Rule, Audrey C.

2015-01-01

Gifted learners need opportunities for critical and creative thinking to stretch their minds and imaginations. Strategies for increasing complexity in the four core areas of language arts, mathematics, science, and social studies were addressed using the Common Core and Iowa Core Standards through several methods. Descriptive adjective object…

Characterization of Hepatitis C Virus Core Protein Multimerization and Membrane Envelopment: Revelation of a Cascade of Core-Membrane Interactions ▿

PubMed Central

Ai, Li-Shuang; Lee, Yu-Wen; Chen, Steve S.-L.

2009-01-01

The molecular basis underlying hepatitis C virus (HCV) core protein maturation and morphogenesis remains elusive. We characterized the concerted events associated with core protein multimerization and interaction with membranes. Analyses of core proteins expressed from a subgenomic system showed that the signal sequence located between the core and envelope glycoprotein E1 is critical for core association with endoplasmic reticula (ER)/late endosomes and the core's envelopment by membranes, which was judged by the core's acquisition of resistance to proteinase K digestion. Despite exerting an inhibitory effect on the core's association with membranes, (Z-LL)2-ketone, a specific inhibitor of signal peptide peptidase (SPP), did not affect core multimeric complex formation, suggesting that oligomeric core complex formation proceeds prior to or upon core attachment to membranes. Protease-resistant core complexes that contained both innate and processed proteins were detected in the presence of (Z-LL)2-ketone, implying that core envelopment occurs after intramembrane cleavage. Mutations of the core that prevent signal peptide cleavage or coexpression with an SPP loss-of-function D219A mutant decreased the core's envelopment, demonstrating that SPP-mediated cleavage is required for core envelopment. Analyses of core mutants with a deletion in domain I revealed that this domain contains sequences crucial for core envelopment. The core proteins expressed by infectious JFH1 and Jc1 RNAs in Huh7 cells also assembled into a multimeric complex, associated with ER/late-endosomal membranes, and were enveloped by membranes. Treatment with (Z-LL)2-ketone or coexpression with D219A mutant SPP interfered with both core envelopment and infectious HCV production, indicating a critical role of core envelopment in HCV morphogenesis. The results provide mechanistic insights into the sequential and coordinated processes during the association of the HCV core protein with membranes in the early phase of virus maturation and morphogenesis. PMID:19605478

Integrative structure and functional anatomy of a nuclear pore complex

NASA Astrophysics Data System (ADS)

Kim, Seung Joong; Fernandez-Martinez, Javier; Nudelman, Ilona; Shi, Yi; Zhang, Wenzhu; Raveh, Barak; Herricks, Thurston; Slaughter, Brian D.; Hogan, Joanna A.; Upla, Paula; Chemmama, Ilan E.; Pellarin, Riccardo; Echeverria, Ignacia; Shivaraju, Manjunatha; Chaudhury, Azraa S.; Wang, Junjie; Williams, Rosemary; Unruh, Jay R.; Greenberg, Charles H.; Jacobs, Erica Y.; Yu, Zhiheng; de La Cruz, M. Jason; Mironska, Roxana; Stokes, David L.; Aitchison, John D.; Jarrold, Martin F.; Gerton, Jennifer L.; Ludtke, Steven J.; Akey, Christopher W.; Chait, Brian T.; Sali, Andrej; Rout, Michael P.

2018-03-01

Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

Integrative structure and functional anatomy of a nuclear pore complex.

PubMed

Kim, Seung Joong; Fernandez-Martinez, Javier; Nudelman, Ilona; Shi, Yi; Zhang, Wenzhu; Raveh, Barak; Herricks, Thurston; Slaughter, Brian D; Hogan, Joanna A; Upla, Paula; Chemmama, Ilan E; Pellarin, Riccardo; Echeverria, Ignacia; Shivaraju, Manjunatha; Chaudhury, Azraa S; Wang, Junjie; Williams, Rosemary; Unruh, Jay R; Greenberg, Charles H; Jacobs, Erica Y; Yu, Zhiheng; de la Cruz, M Jason; Mironska, Roxana; Stokes, David L; Aitchison, John D; Jarrold, Martin F; Gerton, Jennifer L; Ludtke, Steven J; Akey, Christopher W; Chait, Brian T; Sali, Andrej; Rout, Michael P

2018-03-22

Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

Convective cooling in a pool-type research reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sipaun, Susan, E-mail: susan@nm.gov.my; Usman, Shoaib, E-mail: usmans@mst.edu

2016-01-22

A reactor produces heat arising from fission reactions in the nuclear core. In the Missouri University of Science and Technology research reactor (MSTR), this heat is removed by natural convection where the coolant/moderator is demineralised water. Heat energy is transferred from the core into the coolant, and the heated water eventually evaporates from the open pool surface. A secondary cooling system was installed to actively remove excess heat arising from prolonged reactor operations. The nuclear core consists of uranium silicide aluminium dispersion fuel (U{sub 3}Si{sub 2}Al) in the form of rectangular plates. Gaps between the plates allow coolant to passmore » through and carry away heat. A study was carried out to map out heat flow as well as to predict the system’s performance via STAR-CCM+ simulation. The core was approximated as porous media with porosity of 0.7027. The reactor is rated 200kW and total heat density is approximately 1.07+E7 Wm{sup −3}. An MSTR model consisting of 20% of MSTR’s nuclear core in a third of the reactor pool was developed. At 35% pump capacity, the simulation results for the MSTR model showed that water is drawn out of the pool at a rate 1.28 kg s{sup −1} from the 4” pipe, and predicted pool surface temperature not exceeding 30°C.« less

The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events.

PubMed

Sørensen, Brian B; Ehrnsberger, Hans F; Esposito, Silvia; Pfab, Alexander; Bruckmann, Astrid; Hauptmann, Judith; Meister, Gunter; Merkl, Rainer; Schubert, Thomas; Längst, Gernot; Melzer, Michael; Grasser, Marion; Grasser, Klaus D

2017-02-01

We identify proteins that associate with the THO core complex, and show that the TEX1 and MOS11 components functionally interact, affecting mRNA export and splicing as well as plant development. TREX (TRanscription-EXport) is a multiprotein complex that plays a central role in the coordination of synthesis, processing and nuclear export of mRNAs. Using targeted proteomics, we identified proteins that associate with the THO core complex of Arabidopsis TREX. In addition to the RNA helicase UAP56 and the mRNA export factors ALY2-4 and MOS11 we detected interactions with the mRNA export complex TREX-2 and multiple spliceosomal components. Plants defective in the THO component TEX1 or in the mRNA export factor MOS11 (orthologue of human CIP29) are mildly affected. However, tex1 mos11 double-mutant plants show marked defects in vegetative and reproductive development. In tex1 plants, the levels of tasiRNAs are reduced, while miR173 levels are decreased in mos11 mutants. In nuclei of mos11 cells increased mRNA accumulation was observed, while no mRNA export defect was detected with tex1 cells. Nevertheless, in tex1 mos11 double-mutants, the mRNA export defect was clearly enhanced relative to mos11. The subnuclear distribution of TEX1 substantially overlaps with that of splicing-related SR proteins and in tex1 plants the ratio of certain alternative splicing events is altered. Our results demonstrate that Arabidopsis TEX1 and MOS11 are involved in distinct steps of the biogenesis of mRNAs and small RNAs, and that they interact regarding some aspects, but act independently in others.

Liquid uranium alloy-helium fission reactor

DOEpatents

Minkov, V.

1984-06-13

This invention describes a nuclear fission reactor which has a core vessel and at least one tandem heat exchanger vessel coupled therewith across upper and lower passages to define a closed flow loop. Nuclear fuel such as a uranium alloy in its liquid phase fills these vessels and flow passages. Solid control elements in the reactor core vessel are adapted to be adjusted relative to one another to control fission reaction of the liquid fuel therein. Moderator elements in the other vessel and flow passages preclude fission reaction therein. An inert gas such as helium is bubbled upwardly through the heat exchanger vessel operable to move the liquid fuel upwardly therein and unidirectionally around the closed loop and downwardly through the core vessel. This helium gas is further directed to heat conversion means outside of the reactor vessels to utilize the heat from the fission reaction to generate useful output. The nuclear fuel operates in the 1200 to 1800/sup 0/C range, and even higher to 2500/sup 0/C.

Modeling Transients and Designing a Passive Safety System for a Nuclear Thermal Rocket Using Relap5

NASA Astrophysics Data System (ADS)

Khatry, Jivan

Long-term high payload missions necessitate the need for nuclear space propulsion. Several nuclear reactor types were investigated by the Nuclear Engine for Rocket Vehicle Application (NERVA) program of National Aeronautics and Space Administration (NASA). Study of planned/unplanned transients on nuclear thermal rockets is important due to the need for long-term missions. A NERVA design known as the Pewee I was selected for this purpose. The following transients were run: (i) modeling of corrosion-induced blockages on the peripheral fuel element coolant channels and their impact on radiation heat transfer in the core, and (ii) modeling of loss-of-flow-accidents (LOFAs) and their impact on radiation heat transfer in the core. For part (i), the radiation heat transfer rate of blocked channels increases while their neighbors' decreases. For part (ii), the core radiation heat transfer rate increases while the flow rate through the rocket system is decreased. However, the radiation heat transfer decreased while there was a complete LOFA. In this situation, the peripheral fuel element coolant channels handle the majority of the radiation heat transfer. Recognizing the LOFA as the most severe design basis accident, a passive safety system was designed in order to respond to such a transient. This design utilizes the already existing tie rod tubes and connects them to a radiator in a closed loop. Hence, this is basically a secondary loop. The size of the core is unchanged. During normal steady-state operation, this secondary loop keeps the moderator cool. Results show that the safety system is able to remove the decay heat and prevent the fuel elements from melting, in response to a LOFA and subsequent SCRAM.

LINCing complex functions at the nuclear envelope

PubMed Central

Rothballer, Andrea; Schwartz, Thomas U.; Kutay, Ulrike

2013-01-01

Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the double membrane of the nuclear envelope (NE) and physically connect nuclear structures to cytoskeletal elements. LINC complexes are envisioned as force transducers in the NE, which facilitate processes like nuclear anchorage and migration, or chromosome movements. The complexes are built from members of two evolutionary conserved families of transmembrane (TM) proteins, the SUN (Sad1/UNC-84) domain proteins in the inner nuclear membrane (INM) and the KASH (Klarsicht/ANC-1/SYNE homology) domain proteins in the outer nuclear membrane (ONM). In the lumen of the NE, the SUN and KASH domains engage in an intimate assembly to jointly form a NE bridge. Detailed insights into the molecular architecture and atomic structure of LINC complexes have recently revealed the molecular basis of nucleo-cytoskeletal coupling. They bear important implications for LINC complex function and suggest new potential and as yet unexplored roles, which the complexes may play in the cell. PMID:23324460

Logging-while-coring method and apparatus

DOEpatents

Goldberg, David S.; Myers, Gregory J.

2007-11-13

A method and apparatus for downhole coring while receiving logging-while-drilling tool data. The apparatus includes core collar and a retrievable core barrel. The retrievable core barrel receives core from a borehole which is sent to the surface for analysis via wireline and latching tool The core collar includes logging-while-drilling tools for the simultaneous measurement of formation properties during the core excavation process. Examples of logging-while-drilling tools include nuclear sensors, resistivity sensors, gamma ray sensors, and bit resistivity sensors. The disclosed method allows for precise core-log depth calibration and core orientation within a single borehole, and without at pipe trip, providing both time saving and unique scientific advantages.

Logging-while-coring method and apparatus

DOEpatents

Goldberg, David S.; Myers, Gregory J.

2007-01-30

A method and apparatus for downhole coring while receiving logging-while-drilling tool data. The apparatus includes core collar and a retrievable core barrel. The retrievable core barrel receives core from a borehole which is sent to the surface for analysis via wireline and latching tool The core collar includes logging-while-drilling tools for the simultaneous measurement of formation properties during the core excavation process. Examples of logging-while-drilling tools include nuclear sensors, resistivity sensors, gamma ray sensors, and bit resistivity sensors. The disclosed method allows for precise core-log depth calibration and core orientation within a single borehole, and without at pipe trip, providing both time saving and unique scientific advantages.

Molecular Structure of the Pyruvate Dehydrogenase Complex from Escherichia coli K-12

PubMed Central

Vogel, Otto; Hoehn, Barbara; Henning, Ulf

1972-01-01

The pyruvate dehydrogenase core complex from E. coli K-12, defined as the multienzyme complex that can be obtained with a unique polypeptide chain composition, has a molecular weight of 3.75 × 106. All results obtained agree with the following numerology. The core complex consists of 48 polypeptide chains. There are 16 chains (molecular weight = 100,000) of the pyruvate dehydrogenase component, 16 chains (molecular weight = 80,000) of the dihydrolipoamide dehydrogenase component, and 16 chains (molecular weight = 56,000) of the dihydrolipoamide dehydrogenase component. Usually, but not always, pyruvate dehydrogenase complex is produced in vivo containing at least 2-3 mol more of dimers of the pyruvate dehydrogenase component than the stoichiometric ratio with respect to the core complex. This “excess” component is bound differently than are the eight dimers in the core complex. Images PMID:4556465

Common and distinctive localization patterns of Crumbs polarity complex proteins in the mammalian eye.

PubMed

Kim, Jin Young; Song, Ji Yun; Karnam, Santi; Park, Jun Young; Lee, Jamie J H; Kim, Seonhee; Cho, Seo-Hee

2015-01-01

Crumbs polarity complex proteins are essential for cellular and tissue polarity, and for adhesion of epithelial cells. In epithelial tissues deletion of any of three core proteins disrupts localization of the other proteins, indicating structural and functional interdependence among core components. Despite previous studies of function and co-localization that illustrated the properties that these proteins share, it is not known whether an individual component of the complex plays a distinct role in a unique cellular and developmental context. In order to investigate this question, we primarily used confocal imaging to determine the expression and subcellular localization of the core Crumbs polarity complex proteins during ocular development. Here we show that in developing ocular tissues core Crumbs polarity complex proteins, Crb, Pals1 and Patj, generally appear in an overlapping pattern with some exceptions. All three core complex proteins localize to the apical junction of the retinal and lens epithelia. Pals1 is also localized in the Golgi of the retinal cells and Patj localizes to the nuclei of the apically located subset of progenitor cells. These findings suggest that core Crumbs polarity complex proteins exert common and independent functions depending on cellular context. Copyright © 2015 Elsevier B.V. All rights reserved.

RADIATION FACILITY FOR NUCLEAR REACTORS

DOEpatents

Currier, E.L. Jr.; Nicklas, J.H.

1961-12-12

A radiation facility is designed for irradiating samples in close proximity to the core of a nuclear reactor. The facility comprises essentially a tubular member extending through the biological shield of the reactor and containing a manipulatable rod having the sample carrier at its inner end, the carrier being longitudinally movable from a position in close proximity to the reactor core to a position between the inner and outer faces of the shield. Shield plugs are provided within the tubular member to prevent direct radiation from the core emanating therethrough. In this device, samples may be inserted or removed during normal operation of the reactor without exposing personnel to direct radiation from the reactor core. A storage chamber is also provided within the radiation facility to contain an irradiated sample during the period of time required to reduce the radioactivity enough to permit removal of the sample for external handling. (AEC)

A New Innovative Spherical Cermet Nuclear Fuel Element to Achieve an Ultra-Long Core Life for use in Grid-Appropriate LWRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Senor, David J.; Painter, Chad L.; Geelhood, Ken J.

2007-12-01

Spherical cermet fuel elements are proposed for use in the Atoms For Peace Reactor (AFPR-100) concept. AFPR-100 is a small-scale, inherently safe, proliferation-resistant reactor that would be ideal for deployment to nations with emerging economies that decide to select nuclear power for the generation of carbon-free electricity. The basic concept of the AFPR core is a water-cooled fixed particle bed, randomly packed with spherical fuel elements. The flow of coolant within the particle bed is at such a low rate that the bed does not fluidize. This report summarizes an approach to fuel fabrication, results associated with fuel performance modeling,more » core neutronics and thermal hydraulics analyses demonstrating a ~20 year core life, and a conclusion that the proliferation resistance of the AFPR reactor concept is high.« less

An End-To-End Test of A Simulated Nuclear Electric Propulsion System

NASA Technical Reports Server (NTRS)

VanDyke, Melissa; Hrbud, Ivana; Goddfellow, Keith; Rodgers, Stephen L. (Technical Monitor)

2002-01-01

The Safe Affordable Fission Engine (SAFE) test series addresses Phase I Space Fission Systems issues in it particular non-nuclear testing and system integration issues leading to the testing and non-nuclear demonstration of a 400-kW fully integrated flight unit. The first part of the SAFE 30 test series demonstrated operation of the simulated nuclear core and heat pipe system. Experimental data acquired in a number of different test scenarios will validate existing computational models, demonstrated system flexibility (fast start-ups, multiple start-ups/shut downs), simulate predictable failure modes and operating environments. The objective of the second part is to demonstrate an integrated propulsion system consisting of a core, conversion system and a thruster where the system converts thermal heat into jet power. This end-to-end system demonstration sets a precedent for ground testing of nuclear electric propulsion systems. The paper describes the SAFE 30 end-to-end system demonstration and its subsystems.

Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopy.

PubMed

Scheuring, Simon; Busselez, Johan; Lévy, Daniel

2005-01-14

We have studied photosynthetic membranes of wild type Rhodobacter blasticus, a closely related strain to the well studied Rhodobacter sphaeroides, using atomic force microscopy. High-resolution atomic force microscopy topographs of both cytoplasmic and periplasmic surfaces of LH2 and RC-LH1-PufX (RC, reaction center) complexes were acquired in situ. The LH2 is a nonameric ring inserted into the membrane with the 9-fold axis perpendicular to the plane. The core complex is an S-shaped dimer composed of two RCs, each encircled by 13 LH1 alpha/beta-heterodimers, and two PufXs. The LH1 assembly is an open ellipse with a topography-free gap of approximately 25 A. The two PufXs, one of each core, are located at the dimer center. Based on our data, we propose a model of the core complex, which provides explanation for the PufX-induced dimerization of the Rhodobacter core complex. The QB site is located facing a approximately 25-A wide gap within LH1, explaining the PufX-favored quinone passage in and out of the core complex.

Few-body semiclassical approach to nucleon transfer and emission reactions

NASA Astrophysics Data System (ADS)

Sultanov, Renat A.; Guster, D.

2014-04-01

A three-body semiclassical model is proposed to describe the nucleon transfer and emission reactions in a heavy-ion collision. In this model the two heavy particles, i.e. nuclear cores A1(ZA1, MA1) and A2(ZA2, MA2), move along classical trajectories {{R}_1}( t ) and {{R}_2}( t ) respectively, while the dynamics of the lighter neutron (n) is considered from a quantum mechanical point of view. Here, Mi are the nucleon masses and Zi are the Coulomb charges of the heavy nuclei (i = 1, 2). A Faddeev-type semiclassical formulation using realistic paired nuclear-nuclear potentials is applied so that all three channels (elastic, rearrangement and break-up) are described in a unified manner. In order to solve the time-dependent equations the Faddeev components of the total three-body wave-function are expanded in terms of the input and output channel target eigenfunctions. In the special case, when the nuclear cores are identical (A1 ≡ A2) and also the two-level approximation in the expansion over the target (subsystem) functions is used, the time-dependent semiclassical Faddeev equations are resolved in an explicit way. To determine the realistic {{R}_1}( t ) and {{R}_2}( t ) trajectories of the nuclear cores, a self-consistent approach based on the Feynman path integral theory is applied.

Global risk of radioactive fallout after nuclear reactor accidents

NASA Astrophysics Data System (ADS)

Lelieveld, J.; Kunkel, D.; Lawrence, M. G.

2011-11-01

Reactor core meltdowns of nuclear power plants are rare, yet the consequences are catastrophic. But what is meant by "rare"? And what can be learned from the Chernobyl and Fukushima incidents? Here we assess the risk of exposure to radioactivity due to atmospheric dispersion of gases and particles following severe nuclear accidents, using particulate 137Cs and gaseous 131I as proxies for the fallout. It appears that previously the occurrence of major accidents and the risks of radioactive contamination have been underestimated. Using a global model of the atmosphere we compute that on average, in the event of a core melt of any nuclear power plant worldwide, more than 90% of emitted 137Cs would be transported beyond 50km and about 50% beyond 1000 km distance. This corroborates that such accidents have large-scale and trans-boundary impacts. Although the emission strengths and atmospheric removal processes of 137Cs and 131I are quite different, the radioactive contamination patterns over land and the human deposition exposure are computed to be similar. High human exposure risks occur around reactors in densely populated regions, notably in southern Asia where a core melt can subject 55 million people to radioactive contamination. The recent decision by Germany to phase out its nuclear reactors will reduce the national risk, though a large risk will still remain from the reactors in neighbouring countries.

Global risk of radioactive fallout after nuclear reactor accidents

NASA Astrophysics Data System (ADS)

Kunkel, D.; Lelieveld, J.; Lawrence, M. G.

2012-04-01

Reactor core meltdowns of nuclear power plants are rare, yet the consequences are catastrophic. But what is meant by "rare"? And what can be learned from the Chernobyl and Fukushima incidents? Here we assess the risk of exposure to radioactivity due to atmospheric dispersion of gases and particles following severe nuclear accidents, using particulate 137Cs and gaseous 131I as proxies for the fallout. It appears that previously the occurrence of major accidents and the risks of radioactive contamination have been underestimated. Using a global model of the atmosphere we compute that on average, in the event of a core melt of any nuclear power plant worldwide, more than 90 % of emitted 137Cs would be transported beyond 50 km and about 50 % beyond 1000 km distance. This corroborates that such accidents have large-scale and trans-boundary impacts. Although the emission strengths and atmospheric removal processes of 137Cs and 131I are quite different, the radioactive contamination patterns over land and the human deposition exposure are computed to be similar. High human exposure risks occur around reactors in densely populated regions, notably in southern Asia where a core melt can subject 55 million people to radioactive contamination. The recent decision by Germany to phase out its nuclear reactors will reduce the national risk, though a large risk will still remain from the reactors in neighbouring countries.

Design of a Resistively Heated Thermal Hydraulic Simulator for Nuclear Rocket Reactor Cores

NASA Technical Reports Server (NTRS)

Litchford, Ron J.; Foote, John P.; Ramachandran, Narayanan; Wang, Ten-See; Anghaie, Samim

2007-01-01

A preliminary design study is presented for a non-nuclear test facility which uses ohmic heating to replicate the thermal hydraulic characteristics of solid core nuclear reactor fuel element passages. The basis for this testing capability is a recently commissioned nuclear thermal rocket environments simulator, which uses a high-power, multi-gas, wall-stabilized constricted arc-heater to produce high-temperature pressurized hydrogen flows representative of reactor core environments, excepting radiation effects. Initially, the baseline test fixture for this non-nuclear environments simulator was configured for long duration hot hydrogen exposure of small cylindrical material specimens as a low cost means of evaluating material compatibility. It became evident, however, that additional functionality enhancements were needed to permit a critical examination of thermal hydraulic effects in fuel element passages. Thus, a design configuration was conceived whereby a short tubular material specimen, representing a fuel element passage segment, is surrounded by a backside resistive tungsten heater element and mounted within a self-contained module that inserts directly into the baseline test fixture assembly. With this configuration, it becomes possible to create an inward directed radial thermal gradient within the tubular material specimen such that the wall-to-gas heat flux characteristics of a typical fuel element passage are effectively simulated. The results of a preliminary engineering study for this innovative concept are fully summarized, including high-fidelity multi-physics thermal hydraulic simulations and detailed design features.

Nuclear-Localized and Deregulated Calcium- and Calmodulin-Dependent Protein Kinase Activates Rhizobial and Mycorrhizal Responses in Lotus japonicus[W

PubMed Central

Takeda, Naoya; Maekawa, Takaki; Hayashi, Makoto

2012-01-01

The common symbiosis pathway is at the core of symbiosis signaling between plants and soil microbes. In this pathway, calcium- and calmodulin-dependent protein kinase (CCaMK) plays a crucial role in integrating the signals both in arbuscular mycorrhizal symbiosis (AMS) and in root nodule symbiosis (RNS). However, the molecular mechanism by which CCaMK coordinates AMS and RNS is largely unknown. Here, we report that the gain-of-function (GOF) variants of CCaMK without the regulatory domains activate both AMS and RNS signaling pathways in the absence of symbiotic partners. This activation requires nuclear localization of CCaMK. Enforced nuclear localization of the GOF-CCaMK variants by fusion with a canonical nuclear localization signal enhances signaling activity of AMS and RNS. The GOF-CCaMK variant triggers formation of a structure similar to the prepenetration apparatus, which guides infection of arbuscular mycorrhizal fungi to host root cells. In addition, the GOF-CCaMK variants without the regulatory domains partly restore AMS but fail to support rhizobial infection in ccamk mutants. These data indicate that AMS, the more ancient type of symbiosis, can be mainly regulated by the kinase activity of CCaMK, whereas RNS, which evolved more recently, requires complex regulation performed by the regulatory domains of CCaMK. PMID:22337918

Simple and complex hyperplastic papillary proliferations of the endometrium: a clinicopathologic study of nine cases of apparently localized papillary lesions with fibrovascular stromal cores and epithelial metaplasia.

PubMed

Lehman, M B; Hart, W R

2001-11-01

The clinicopathologic features of nine cases of papillary proliferation of the endometrium devoid of malignant nuclear features were studied. The patients ranged in age from 33 to 71 years (median 57 years). All were postmenopausal, except the youngest. The most common symptom was postmenopausal bleeding. Two patients were receiving hormonal replacement therapy and two were taking megestrol acetate. Two lesions were incidental findings in a hysterectomy specimen. Seven were diagnosed in endometrial biopsy or curettage specimens. In six cases (67%) the lesion involved an endometrial polyp. In all cases the papillae had fibrovascular stromal cores and variable degrees of branching. Two architectural patterns were found. A simple papillary pattern with involvement of only a few glands and little epithelial proliferation occurred in five cases, including three that were entirely intracystic. A complex papillary pattern with more extensive involvement of endometrial glands, a greater degree of branching of the papillae, and cellular tufting occurred in four cases. One or more metaplastic epithelial changes occurred in all cases, including endocervical-type mucinous metaplasia in nine cases (90%), eosinophilic cell change in eight (89%), ciliated cell change in seven (70%), focal squamous metaplasia in two cases (22%), and hobnail cell change in two (22%). Mitotic figures were found in three cases. In four lesions (44%), all with a complex papillary pattern, the proliferating cells had mild nuclear atypia. Three of these patients underwent hysterectomy within 5 months. Simple nonpapillary hyperplasia and two endometrial polyps were found in one patient, complex nonpapillary hyperplasia in one, and atrophic endometrium in the other. Two patients had additional endometrial samplings within 4 months that contained small residual simple papillary lesions. One of these had another biopsy at 16 months that showed only atrophy. One patient had no subsequent diagnostic or therapeutic procedures. One patient was a recent case. Of the three patients with intact uteri and appreciable follow-up, all were alive and well at 14, 96, and 102 months, respectively. We conclude that these papillary proliferations are a form of hyperplasia that is closely associated with endometrial epithelial metaplasia. Polypectomy and/or curettage may be effective in removing them because they often are localized lesions. Although all of our patients had an uneventful outcome, the number of cases is small. Our findings question the validity of diagnosing endometrial lesions as well-differentiated carcinoma solely because of a complex papillary architectural pattern.

Constraining Nuclear Weak Interactions in Astrophysics and New Many-Core Algorithms for Neuroevolution

NASA Astrophysics Data System (ADS)

Sullivan, Christopher James

Weak interactions involving atomic nuclei are critical components in a broad range of as- trophysical phenomenon. As allowed Gamow-Teller transitions are the primary path through which weak interactions in nuclei operate in astrophysical contexts, the constraint of these nuclear transitions is an important goal of nuclear astrophysics. In this work, the charged current nuclear weak interaction known as electron capture is studied in the context of stellar core-collapse supernovae (CCSNe). Specifically, the sensitivity of the core-collapse and early post-bounce phases of CCSNe to nuclear electron capture rates are examined. Electron capture rates are adjusted by factors consistent with uncer- tainties indicated by comparing theoretical rates to those deduced from charge-exchange and beta-decay measurements. With the aide of such sensitivity studies, the diverse role of electron capture on thousands of nuclear species is constrained to a few tens of nuclei near N 50 and A 80 which dictate the primary response of CCSNe to nuclear electron capture. As electron capture is shown to be a leading order uncertainty during the core-collapse phase of CCSNe, future experimental and theoretical efforts should seek to constrain the rates of nuclei in this region. Furthermore, neutral current neutrino-nuclear interactions in the tens-of-MeV energy range are important in a variety of astrophysical environments including core-collapse super- novae as well as in the synthesis of some of the solar systems rarest elements. Estimates for inelastic neutrino scattering on nuclei are also important for neutrino detector construction aimed at the detection of astrophysical neutrinos. Due to the small cross sections involved, direct measurements are rare and have only been performed on a few nuclei. For this rea- son, indirect measurements provide a unique opportunity to constrain the nuclear transition strength needed to infer inelastic neutrino-nucleus cross sections. Herein the (6Li, 6Li‧) inelastic scattering reaction at 100 MeV/u is shown to indirectly select the relevant transitions for inelastic neutrino-nucleus scattering. Specifically, the probes unique selectivity of isovector- spin transfer excitations (Delta S = 1, DeltaT = 1, DeltaTz = 0) is demonstrated, thereby allowing the extraction of Gamow-Teller transition strength in the inelastic channel. Finally, the development and performance of a newly established technique for the sub- field of artificial intelligence known as neuroevolution is described. While separate from the physics that is discussed, these algorithmic advancements seek to improve the adoption of machine learning in the scientific domain by enabling neuroevolution to take advantage of modern heterogeneous compute architectures. Because the evolution of neural network pop- ulations offloads the choice of specific details about the neural networks to an evolutionary search algorithm, neuroevolution can increase the accessibility of machine learning. However, the evolution of neural networks through parameter and structural space presents a novel di- vergence problem when mapping the evaluation of these networks to many-core architectures. The principal focus of the algorithm optimizations described herein are on improving the feed-forward evaluation time when tens-to-hundreds of thousands of heterogeneous neural networks are evaluated concurrently.

Cooling system for a nuclear reactor

DOEpatents

Amtmann, Hans H.

1982-01-01

A cooling system for a gas-cooled nuclear reactor is disclosed which includes at least one primary cooling loop adapted to pass coolant gas from the reactor core and an associated steam generator through a duct system having a main circulator therein, and at least one auxiliary cooling loop having communication with the reactor core and adapted to selectively pass coolant gas through an auxiliary heat exchanger and circulator. The main and auxiliary circulators are installed in a common vertical cavity in the reactor vessel, and a common return duct communicates with the reactor core and intersects the common cavity at a junction at which is located a flow diverter valve operative to effect coolant flow through either the primary or auxiliary cooling loops.

Nuclear reactor composite fuel assembly

DOEpatents

Burgess, Donn M.; Marr, Duane R.; Cappiello, Michael W.; Omberg, Ronald P.

1980-01-01

A core and composite fuel assembly for a liquid-cooled breeder nuclear reactor including a plurality of elongated coextending driver and breeder fuel elements arranged to form a generally polygonal bundle within a thin-walled duct. The breeder elements are larger in cross section than the driver elements, and each breeder element is laterally bounded by a number of the driver elements. Each driver element further includes structure for spacing the driver elements from adjacent fuel elements and, where adjacent, the thin-walled duct. A core made up of the fuel elements can advantageously include fissile fuel of only one enrichment, while varying the effective enrichment of any given assembly or core region, merely by varying the relative number and size of the driver and breeder elements.

NUCLEAR REACTOR CONTROL SYSTEM

DOEpatents

Howard, D.F.; Motta, E.E.

1961-06-27

A method for controlling the excess reactivity in a nuclear reactor throughout the core life while maintaining the neutron flux distribution at the desired level is described. The control unit embodies a container having two electrodes of different surface area immersed in an electrolytic solution of a good neutron sbsorbing metal ion such as boron, gadolinium, or cadmium. Initially, the neutron absorber is plated on the larger electrode to control the greater neutron flux of a freshly refueled core. As the fuel burns up, the excess reactivity decreases and the neutron absorber is then plated onto the smaller electrode so that the number of neutrons absorbed also decreases. The excess reactivity in the core may thus be maintained without the introduction of serious perturbations in the neutron flux distributibn.

Reactivity control assembly for nuclear reactor

DOEpatents

Bollinger, Lawrence R.

1984-01-01

Reactivity control assembly for nuclear reactor comprises supports stacked above reactor core for holding control rods. Couplers associated with the supports and a vertically movable drive shaft have lugs at their lower ends for engagement with the supports.

Nuclear reactor neutron shielding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Speaker, Daniel P; Neeley, Gary W; Inman, James B

A nuclear reactor includes a reactor pressure vessel and a nuclear reactor core comprising fissile material disposed in a lower portion of the reactor pressure vessel. The lower portion of the reactor pressure vessel is disposed in a reactor cavity. An annular neutron stop is located at an elevation above the uppermost elevation of the nuclear reactor core. The annular neutron stop comprises neutron absorbing material filling an annular gap between the reactor pressure vessel and the wall of the reactor cavity. The annular neutron stop may comprise an outer neutron stop ring attached to the wall of the reactormore » cavity, and an inner neutron stop ring attached to the reactor pressure vessel. An excore instrument guide tube penetrates through the annular neutron stop, and a neutron plug comprising neutron absorbing material is disposed in the tube at the penetration through the neutron stop.« less

Symplectic no-core configuration interaction framework for ab initio nuclear structure. II. Structure of rotational states

NASA Astrophysics Data System (ADS)

Caprio, Mark A.; McCoy, Anna E.; Dytrych, Tomas

2017-09-01

Rotational band structure is readily apparent as an emergent phenomenon in ab initio nuclear many-body calculations of light nuclei, despite the incompletely converged nature of most such calculations at present. Nuclear rotation in light nuclei can be analyzed in terms of approximate dynamical symmetries of the nuclear many-body problem: in particular, Elliott's SU (3) symmetry of the three-dimensional harmonic oscillator and the symplectic Sp (3 , R) symmetry of three-dimensional phase space. Calculations for rotational band members in the ab initio symplectic no-core configuration interaction (SpNCCI) framework allow us to directly examine the SU (3) and Sp (3 , R) nature of rotational states. We present results for rotational bands in p-shell nuclei. Supported by the US DOE under Award No. DE-FG02-95ER-40934 and the Czech Science Foundation under Grant No. 16-16772S.

REACTOR CONTROL ROD OPERATING SYSTEM

DOEpatents

Miller, G.

1961-12-12

A nuclear reactor control rod mechanism is designed which mechanically moves the control rods into and out of the core under normal conditions but rapidly forces the control rods into the core by catapultic action in the event of an emergency. (AEC)

Selection of Nuclear Fuel for TREAT: UO 2 vs U 3O 8

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glazoff, Michael Vasily; Van Rooyen, Isabella Johanna; Coryell, Benjamin David

The Transient Reactor Test (TREAT) that resides at the Materials and Fuels Complex (MFC) at Idaho National Laboratory (INL), first achieved criticality in 1959, and successfully performed many transient tests on nuclear fuel until 1994 when its operations were suspended. Resumption of operations at TREAT was approved in February 2014 to meet the U.S. Department of Energy (DOE) Office of Nuclear Energy’s objectives in transient testing of nuclear fuels. The National Nuclear Security Administration’s is converting TREAT from its existing highly enriched uranium (HEU) core to a new core containing low enriched uranium (LEU) (i.e., U-235< 20% by weight). Themore » TREAT Conversion project is currently progressing with conceptual design phase activities. Dimensional stability of the fuel element assemblies, predictable fuel can oxidation and sufficient heat conductivity by the fuel blocks are some of the critical performance requirements of the new LEU fuel. Furthermore, to enable the design team to design fuel block and can specifications, it is amongst the objectives to evaluate TREAT LEU fuel and cladding material’s chemical interaction. This information is important to understand the viability of Zr-based alloys and fuel characteristics for the fabrication of the TREAT LEU fuel and cladding. Also, it is very important to make the right decision on what type of nuclear fuel will be used at TREAT. In particular, one has to consider different oxides of uranium, and most importantly, UO 2 vs U 3O 8. In this report, the results are documented pertaining to the choice mentioned above (UO 2 vs U 3O 8). The conclusion in favor of using UO 2 was made based on the analysis of historical data, up-to-date literature, and self-consistent calculations of phase equilibria and thermodynamic properties in the U-O and U-O-C systems. The report is organized as follows. First, the criteria that were used to make the choice are analyzed. Secondly, existing historical data and current literature were reviewed. This analysis was supplemented by the construction and examination of the U-O and U-O-C phase diagrams at pressure close to negligent, thereby mimicking the conditions in which nuclear fuel is supposed to function inside the zirconium-based cladding in the reactor. Finally, our conclusion in favor of the UO 2 down selection was summarized and explained in the last Section of this document.« less

Mechanistic Insights from Structural Analyses of Ran-GTPase-Driven Nuclear Export of Proteins and RNAs.

PubMed

Matsuura, Yoshiyuki

2016-05-22

Understanding how macromolecules are rapidly exchanged between the nucleus and the cytoplasm through nuclear pore complexes is a fundamental problem in biology. Exportins are Ran-GTPase-dependent nuclear transport factors that belong to the karyopherin-β family and mediate nuclear export of a plethora of proteins and RNAs, except for bulk mRNA nuclear export. Exportins bind cargo macromolecules in a Ran-GTP-dependent manner in the nucleus, forming exportin-cargo-Ran-GTP complexes (nuclear export complexes). Transient weak interactions between exportins and nucleoporins containing characteristic FG (phenylalanine-glycine) repeat motifs facilitate nuclear pore complex passage of nuclear export complexes. In the cytoplasm, nuclear export complexes are disassembled, thereby releasing the cargo. GTP hydrolysis by Ran promoted in the cytoplasm makes the disassembly reaction virtually irreversible and provides thermodynamic driving force for the overall export reaction. In the past decade, X-ray crystallography of some of the exportins in various functional states coupled with functional analyses, single-particle electron microscopy, molecular dynamics simulations, and small-angle solution X-ray scattering has provided rich insights into the mechanism of cargo binding and release and also begins to elucidate how exportins interact with the FG repeat motifs. The knowledge gained from structural analyses of nuclear export is being translated into development of clinically useful inhibitors of nuclear export to treat human diseases such as cancer and influenza. Copyright © 2015 Elsevier Ltd. All rights reserved.

Muon trackers for imaging a nuclear reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kume, N.; Miyadera, H.; Morris, C. L.

A detector system for assessing damage to the cores of the Fukushima Daiichi nuclear reactors by using cosmic-ray muon tomography was developed. Furthermore, the system consists of a pair of drift-tube tracking detectors of 7.2× 7.2-m 2 area. In each muon tracker there consists 6 x-layer and 6 y-layer drift-tube detectors. Each tracker is capable of measuring muon tracks with 12 mrad angular resolutions, and is capable of operating under 50-μ Sv/h radiation environment by removing gamma induced background with a novel time-coincidence logic. An estimated resolution to observe nuclear fuel debris at Fukushima Daiichi is 0.3 m when themore » core is imaged from outside the reactor building.« less

Muon trackers for imaging a nuclear reactor

DOE PAGES

Kume, N.; Miyadera, H.; Morris, C. L.; ...

2016-09-21

A detector system for assessing damage to the cores of the Fukushima Daiichi nuclear reactors by using cosmic-ray muon tomography was developed. Furthermore, the system consists of a pair of drift-tube tracking detectors of 7.2× 7.2-m 2 area. In each muon tracker there consists 6 x-layer and 6 y-layer drift-tube detectors. Each tracker is capable of measuring muon tracks with 12 mrad angular resolutions, and is capable of operating under 50-μ Sv/h radiation environment by removing gamma induced background with a novel time-coincidence logic. An estimated resolution to observe nuclear fuel debris at Fukushima Daiichi is 0.3 m when themore » core is imaged from outside the reactor building.« less

Nuclear reactor safety research since three mile island.

PubMed

Mynatt, F R

1982-04-09

The Three Mile Island nuclear power plant accident has resulted in redirection of reactor safety research priorities. The small release to the environment of radioactive iodine-13 to 17 curies in a total radioactivity release of 2.4 million to 13 million curies-has led to a new emphasis on the physical chemistry of fission product behavior in accidents; the fact that the nuclear core was severely damaged but did not melt down has opened a new accident regime-that of the degraded core; the role of the operators in the progression and severity of the accident has shifted emphasis from equipment reliability to human reliability. As research progresses in these areas, the technical base for regulation and risk analysis will change substantially.

Wave propagation simulation in the upper core of sodium-cooled fast reactors using a spectral-element method for heterogeneous media

NASA Astrophysics Data System (ADS)

Nagaso, Masaru; Komatitsch, Dimitri; Moysan, Joseph; Lhuillier, Christian

2018-01-01

ASTRID project, French sodium cooled nuclear reactor of 4th generation, is under development at the moment by Alternative Energies and Atomic Energy Commission (CEA). In this project, development of monitoring techniques for a nuclear reactor during operation are identified as a measure issue for enlarging the plant safety. Use of ultrasonic measurement techniques (e.g. thermometry, visualization of internal objects) are regarded as powerful inspection tools of sodium cooled fast reactors (SFR) including ASTRID due to opacity of liquid sodium. In side of a sodium cooling circuit, heterogeneity of medium occurs because of complex flow state especially in its operation and then the effects of this heterogeneity on an acoustic propagation is not negligible. Thus, it is necessary to carry out verification experiments for developments of component technologies, while such kind of experiments using liquid sodium may be relatively large-scale experiments. This is why numerical simulation methods are essential for preceding real experiments or filling up the limited number of experimental results. Though various numerical methods have been applied for a wave propagation in liquid sodium, we still do not have a method for verifying on three-dimensional heterogeneity. Moreover, in side of a reactor core being a complex acousto-elastic coupled region, it has also been difficult to simulate such problems with conventional methods. The objective of this study is to solve these 2 points by applying three-dimensional spectral element method. In this paper, our initial results on three-dimensional simulation study on heterogeneous medium (the first point) are shown. For heterogeneity of liquid sodium to be considered, four-dimensional temperature field (three spatial and one temporal dimension) calculated by computational fluid dynamics (CFD) with Large-Eddy Simulation was applied instead of using conventional method (i.e. Gaussian Random field). This three-dimensional numerical experiment yields that we could verify the effects of heterogeneity of propagation medium on waves in Liquid sodium.

Prism. Volume 4, Number 1

DTIC Science & Technology

2012-01-01

Fukushima Daiichi Nuclear Power Plant cannot be regarded as a natural disaster .”12 the report stated that Fukushima was a man-made disaster that... disaster at the Fukushima Daiichi Nuclear Power Plant on March 11, 2012, was a stark reminder that the residual risk of a core meltdown is not so low as...resulting tsunami led to the meltdown of three nuclear reactors at the Fukushima Daiichi Nuclear Power Plant. In the

Monomeric RC-LH1 core complexes retard LH2 assembly and intracytoplasmic membrane formation in PufX-minus mutants of Rhodobacter sphaeroides.

PubMed

Adams, Peter G; Mothersole, David J; Ng, Irene W; Olsen, John D; Hunter, C Neil

2011-09-01

In the model photosynthetic bacterium Rhodobacter sphaeroides domains of light-harvesting 2 (LH2) complexes surround and interconnect dimeric reaction centre-light-harvesting 1-PufX (RC-LH1-PufX) 'core' complexes, forming extensive networks for energy transfer and trapping. These complexes are housed in spherical intracytoplasmic membranes (ICMs), which are assembled in a stepwise process where biosynthesis of core complexes tends to dominate the early stages of membrane invagination. The kinetics of LH2 assembly were measured in PufX mutants that assemble monomeric core complexes, as a consequence of either a twelve-residue N-terminal truncation of PufX (PufXΔ12) or the complete removal of PufX (PufX(-)). Lower rates of LH2 assembly and retarded maturation of membrane invagination were observed for the larger and less curved ICM from the PufX(-) mutant, consistent with the proposition that local membrane curvature, initiated by arrays of bent RC-LH1-PufX dimers, creates a favourable environment for stable assembly of LH2 complexes. Transmission electron microscopy and high-resolution atomic force microscopy were used to examine ICM morphology and membrane protein organisation in these mutants. Some partitioning of core and LH2 complexes was observed in PufX(-) membranes, resulting in locally ordered clusters of monomeric RC-LH1 complexes. The distribution of core and LH2 complexes in the three types of membrane examined is consistent with previous models of membrane curvature and domain formation (Frese et al., 2008), which demonstrated that a combination of crowding and asymmetries in sizes and shapes of membrane protein complexes drives membrane organisation. 2011 Elsevier B.V. All rights reserved.

Briefing Book. Volume 1: The Evolution of the Nuclear Non-Proliferation Regime (Fourth Edition).

DTIC Science & Technology

1998-01-01

usually termed) nuclear reactors. The first of these is that they contain a core or mass of fissile material (the fuel ) which may weigh tens of tons... HTGR is cooled with helium gas and moderated with graphite. Highly enriched uranium is used as fuel (93 per cent U-235), though this may be mixed with...to convert U-238 in a blanket around the core into Pu-239 at a rate faster than its own consumption of fissile material. They thus produce more fuel

Energy production using fission fragment rockets

NASA Astrophysics Data System (ADS)

Chapline, G.; Matsuda, Y.

1991-08-01

Fission fragment rockets are nuclear reactors with a core consisting of thin fibers in a vacuum, and which use magnetic fields to extract the fission fragments from the reactor core. As an alternative to ordinary nuclear reactors, fission fragment rockets would have the following advantages: approximately twice the efficiency if the fission fragment energy can be directly converted into electricity; reduction of the buildup of a fission fragment inventory in the reactor could avoid a Chernobyl type disaster; and collection of the fission fragments outside the reactor could simplify the waste disposal problem.

Nuclear engine flow reactivity shim control

DOEpatents

Walsh, J.M.

1973-12-11

A nuclear engine control system is provided which automatically compensates for reactor reactivity uncertainties at the start of life and reactivity losses due to core corrosion during the reactor life in gas-cooled reactors. The coolant gas flow is varied automatically by means of specially provided control apparatus so that the reactor control drums maintain a predetermined steady state position throughout the reactor life. This permits the reactor to be designed for a constant drum position and results in a desirable, relatively flat temperature profile across the core. (Official Gazette)

Nuclear reactor fuel element with vanadium getter on cladding

DOEpatents

Johnson, Carl E.; Carroll, Kenneth G.

1977-01-01

A nuclear reactor fuel element is described which has an outer cladding, a central core of fissionable or mixed fissionable and fertile fuel material and a layer of vanadium as an oxygen getter on the inner surface of the cladding. The vanadium reacts with oxygen released by the fissionable material during irradiation of the core to prevent the oxygen from reacting with and corroding the cladding. Also described is a method for coating the inner surface of small diameter tubes of cladding with a layer of vanadium.

Comparison of MELCOR and SCDAP/RELAP5 results for a low-pressure, short-term station blackout at Browns Ferry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbajo, J.J.

1995-12-31

This study compares results obtained with two U.S. Nuclear Regulatory Commission (NRC)-sponsored codes, MELCOR version 1.8.3 (1.8PQ) and SCDAP/RELAP5 Mod3.1 release C, for the same transient - a low-pressure, short-term station blackout accident at the Browns Ferry nuclear plant. This work is part of MELCOR assessment activities to compare core damage progression calculations of MELCOR against SCDAP/RELAP5 since the two codes model core damage progression very differently.

Evaluation Metrics Applied to Accident Tolerant Fuels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shannon M. Bragg-Sitton; Jon Carmack; Frank Goldner

2014-10-01

The safe, reliable, and economic operation of the nation’s nuclear power reactor fleet has always been a top priority for the United States’ nuclear industry. Continual improvement of technology, including advanced materials and nuclear fuels, remains central to the industry’s success. Decades of research combined with continual operation have produced steady advancements in technology and have yielded an extensive base of data, experience, and knowledge on light water reactor (LWR) fuel performance under both normal and accident conditions. One of the current missions of the U.S. Department of Energy’s (DOE) Office of Nuclear Energy (NE) is to develop nuclear fuelsmore » and claddings with enhanced accident tolerance for use in the current fleet of commercial LWRs or in reactor concepts with design certifications (GEN-III+). Accident tolerance became a focus within advanced LWR research upon direction from Congress following the 2011 Great East Japan Earthquake, resulting tsunami, and subsequent damage to the Fukushima Daiichi nuclear power plant complex. The overall goal of ATF development is to identify alternative fuel system technologies to further enhance the safety, competitiveness and economics of commercial nuclear power. Enhanced accident tolerant fuels would endure loss of active cooling in the reactor core for a considerably longer period of time than the current fuel system while maintaining or improving performance during normal operations. The U.S. DOE is supporting multiple teams to investigate a number of technologies that may improve fuel system response and behavior in accident conditions, with team leadership provided by DOE national laboratories, universities, and the nuclear industry. Concepts under consideration offer both evolutionary and revolutionary changes to the current nuclear fuel system. Mature concepts will be tested in the Advanced Test Reactor at Idaho National Laboratory beginning in Summer 2014 with additional concepts being readied for insertion in fiscal year 2015. This paper provides a brief summary of the proposed evaluation process that would be used to evaluate and prioritize the candidate accident tolerant fuel concepts currently under development.« less

Evaluating the core microbiota in complex communities: A systematic investigation.

PubMed

Astudillo-García, Carmen; Bell, James J; Webster, Nicole S; Glasl, Bettina; Jompa, Jamaluddin; Montoya, Jose M; Taylor, Michael W

2017-04-01

The study of complex microbial communities poses unique conceptual and analytical challenges, with microbial species potentially numbering in the thousands. With transient or allochthonous microorganisms often adding to this complexity, a 'core' microbiota approach, focusing only on the stable and permanent members of the community, is becoming increasingly popular. Given the various ways of defining a core microbiota, it is prudent to examine whether the definition of the core impacts upon the results obtained. Here we used complex marine sponge microbiotas and undertook a systematic evaluation of the degree to which different factors used to define the core influenced the conclusions. Significant differences in alpha- and beta-diversity were detected using some but not all core definitions. However, findings related to host specificity and environmental quality were largely insensitive to major changes in the core microbiota definition. Furthermore, none of the applied definitions altered our perception of the ecological networks summarising interactions among bacteria within the sponges. These results suggest that, while care should still be taken in interpretation, the core microbiota approach is surprisingly robust, at least for comparing microbiotas of closely related samples. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

Acid-switched Eu(III) coordination inside reverse aggregates: Insights into a synergistic liquid-liquid extraction system

DOE PAGES

Ellis, Ross J.

2016-08-09

Determining the structure of complex solutions bearing metal ions is challenging, but crucial for developing important technologies such as liquid-liquid extraction for metal refining and separation purposes. Herein, the structure of an organic Eu(III) solution consisting a binary mixture of lipophilic ligands di-2-ethylhexyl phosphoric acid (HDEHP) and tetraoctyl diglycolamide (TODGA) in dodecane is studied using synchrotron small angle X-ray scattering (SAXS) and X-ray absorption fine structure spectroscopy (EXAFS). This system is of technological importance in f-element separation for nuclear fuel cycle applications, where extraction is controlled by varying nitric acid concentration. Extraction is promoted at low and high concentration, butmore » is retarded at intermediate concentration, leading to a U-shaped function; the structural origins of which we investigate. At the nanoscale, the solution is apparently comprised of reverse micelles with polar cores of approximately 1 nm in size, and these remain virtually unchanged as acid concentration is varied. Inside the polar cores, the coordination environment of Eu(III) switches from a 9-coordinate [Eu(TODGA) 3] 3+ motif at high acid, to a 6-coordinate HDEHP-dominated complex resembling Eu(HDEHP·DEHP) 3 at low acid. The results show that extraction is controlled within the coordination sphere, where it is promoted under conditions that favor coordination of either one of the two organic ligands, but is retarded under conditions that encourage mixed complexes. Lastly, our results link solution structure with ion transport properties in a technologically-important liquid-liquid ion extraction system.« less

Unsymmetrical Bimetallic Complexes with MII–(μ-OH)–MII Cores (MIIMIII = FeIIFeIII, MnIIFeIII, MnIIMnIII): Structural, Magnetic, and Redox Properties

PubMed Central

Sano, Yohei; Weitz, Andrew C.; Ziller, Joseph W.; Hendrich, Michael P.; Borovik, A.S.

2013-01-01

Heterobimetallic cores are important unit within the active sites of metalloproteins, but are often difficult to duplicate in synthetic systems. We have developed a synthetic approach for the preparation of a complex with a MnII–(μ-OH)–FeIII core, in which the metal centers have different coordination environments. Structural and physical data support the assignment of this complex as a heterobimetallic system. Comparison with the analogous homobimetallic complexes, those containing MnII–(μ-OH)–MnIII and FeII–(μ-OH)–FeIII cores, further supports this assignment. PMID:23992041

Numerical simulations of relativistic heavy-ion reactions

NASA Astrophysics Data System (ADS)

Daffin, Frank Cecil

Bulk quantities of nuclear matter exist only in the compact bodies of the universe. There the crushing gravitational forces overcome the Coulomb repulsion in massive stellar collapses. Nuclear matter is subjected to high pressures and temperatures as shock waves propagate and burn their way through stellar cores. The bulk properties of nuclear matter are important parameters in the evolution of these collapses, some of which lead to nucleosynthesis. The nucleus is rich in physical phenomena. Above the Coulomb barrier, complex interactions lead to the distortion of, and as collision energies increase, the destruction of the nuclear volume. Of critical importance to the understanding of these events is an understanding of the aggregate microscopic processes which govern them. In an effort to understand relativistic heavy-ion reactions, the Boltzmann-Uehling-Uhlenbeck (Ueh33) (BUU) transport equation is used as the framework for a numerical model. In the years since its introduction, the numerical model has been instrumental in providing a coherent, microscopic, physical description of these complex, highly non-linear events. This treatise describes the background leading to the creation of our numerical model of the BUU transport equation, details of its numerical implementation, its application to the study of relativistic heavy-ion collisions, and some of the experimental observables used to compare calculated results to empirical results. The formalism evolves the one-body Wigner phase-space distribution of nucleons in time under the influence of a single-particle nuclear mean field interaction and a collision source term. This is essentially the familiar Boltzmann transport equation whose source term has been modified to address the Pauli exclusion principle. Two elements of the model allow extrapolation from the study of nuclear collisions to bulk quantities of nuclear matter: the modification of nucleon scattering cross sections in nuclear matter, and the compressibility of nuclear matter. Both are primarily subject to the short- range portion of the inter-nucleon potential, and do not show strong finite-size effects. To that end, several useful observables are introduced and their behavior, as BUU model parameters are changed, explored. The average, directed, in-plane, transverse momentum distribution in rapidity is the oldest of the observables presented in this work. Its slope at mid- rapidity is called the flow of the event, and well characterizes the interplay of repulsive and attractive elements of the dynamics of the events. The BUU model has been quite successful in its role of illuminating the physics of intermediate energy heavy-ion collisions. Though current numerical implementations suffer from some shortcomings they have nonetheless served the community well.

Nuclear system that burns its own wastes shows promise

NASA Technical Reports Server (NTRS)

Atchison, K.

1975-01-01

A nuclear fission energy system, capable of eliminating a significant amount of its radioactive wastes by burning them, is described. A theoretical investigation of this system conducted by computer analysis, is based on use of gaseous fuel nuclear reactors. Gaseous core reactors using a uranium plasma fuel are studied along with development for space propulsion.

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.

PubMed

Nguyen, Thi Hoang Duong; Galej, Wojciech P; Fica, Sebastian M; Lin, Pei-Chun; Newman, Andrew J; Nagai, Kiyoshi

2016-02-01

The spliceosome is formed on pre-mRNA substrates from five small nuclear ribonucleoprotein particles (U1, U2, U4/U6 and U5 snRNPs), and numerous non-snRNP factors. Saccharomyces cerevisiae U4/U6.U5 tri-snRNP comprises U5 snRNA, U4/U6 snRNA duplex and approximately 30 proteins and represents a substantial part of the spliceosome before activation. Schizosaccharomyces pombe U2.U6.U5 spliceosomal complex is a post-catalytic intron lariat spliceosome containing U2 and U5 snRNPs, NTC (nineteen complex), NTC-related proteins (NTR), U6 snRNA, and an RNA intron lariat. Two recent papers describe near-complete atomic structures of these complexes based on cryoEM single-particle analysis. The U4/U6.U5 tri-snRNP structure provides crucial insight into the activation mechanism of the spliceosome. The U2.U6.U5 complex reveals the striking architecture of NTC and NTR and important features of the group II intron-like catalytic RNA core remaining after spliced mRNA is released. These two structures greatly advance our understanding of the mechanism of pre-mRNA splicing. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

PROCESS FOR JACKETING A CORE

DOEpatents

Last, G.A.

1960-07-19

A process is given for enclosing the uranium core of a nuclear fuel element by placing the core in an aluminum cup and closing the open end of the cup over the core. As the metal of the cup is brought together in a weld over the center of the end of the core, it is extruded inwardly as internal projection into a central recess in the core and outwardly as an external projection. Thus oxide inclusions in the weld of the cup are spread out into the internal and external projections and do not interfere with the integrity of the weld.

Evidence for a Role of Chloroplastic m-Type Thioredoxins in the Biogenesis of Photosystem II in Arabidopsis1[C][W][OPEN

PubMed Central

Wang, Peng; Liu, Jun; Liu, Bing; Feng, Dongru; Da, Qingen; Wang, Peng; Shu, Shengying; Su, Jianbin; Zhang, Yang; Wang, Jinfa; Wang, Hong-Bin

2013-01-01

Chloroplastic m-type thioredoxins (TRX m) are essential redox regulators in the light regulation of photosynthetic metabolism. However, recent genetic studies have revealed novel functions for TRX m in meristem development, chloroplast morphology, cyclic electron flow, and tetrapyrrole synthesis. The focus of this study is on the putative role of TRX m1, TRX m2, and TRX m4 in the biogenesis of the photosynthetic apparatus in Arabidopsis (Arabidopsis thaliana). To that end, we investigated the impact of single, double, and triple TRX m deficiency on chloroplast development and the accumulation of thylakoid protein complexes. Intriguingly, only inactivation of three TRX m genes led to pale-green leaves and specifically reduced stability of the photosystem II (PSII) complex, implying functional redundancy between three TRX m isoforms. In addition, plants silenced for three TRX m genes displayed elevated levels of reactive oxygen species, which in turn interrupted the transcription of photosynthesis-related nuclear genes but not the expression of chloroplast-encoded PSII core proteins. To dissect the function of TRX m in PSII biogenesis, we showed that TRX m1, TRX m2, and TRX m4 interact physically with minor PSII assembly intermediates as well as with PSII core subunits D1, D2, and CP47. Furthermore, silencing three TRX m genes disrupted the redox status of intermolecular disulfide bonds in PSII core proteins, most notably resulting in elevated accumulation of oxidized CP47 oligomers. Taken together, our results suggest an important role for TRX m1, TRX m2, and TRX m4 proteins in the biogenesis of PSII, and they appear to assist the assembly of CP47 into PSII. PMID:24151299

Automatic safety rod for reactors

DOEpatents

Germer, John H.

1988-01-01

An automatic safety rod for a nuclear reactor containing neutron absorbing material and designed to be inserted into a reactor core after a loss-of-core flow. Actuation is based upon either a sudden decrease in core pressure drop or the pressure drop decreases below a predetermined minimum value. The automatic control rod includes a pressure regulating device whereby a controlled decrease in operating pressure due to reduced coolant flow does not cause the rod to drop into the core.

Identifying protein complex by integrating characteristic of core-attachment into dynamic PPI network.

PubMed

Shen, Xianjun; Yi, Li; Jiang, Xingpeng; He, Tingting; Yang, Jincai; Xie, Wei; Hu, Po; Hu, Xiaohua

2017-01-01

How to identify protein complex is an important and challenging task in proteomics. It would make great contribution to our knowledge of molecular mechanism in cell life activities. However, the inherent organization and dynamic characteristic of cell system have rarely been incorporated into the existing algorithms for detecting protein complexes because of the limitation of protein-protein interaction (PPI) data produced by high throughput techniques. The availability of time course gene expression profile enables us to uncover the dynamics of molecular networks and improve the detection of protein complexes. In order to achieve this goal, this paper proposes a novel algorithm DCA (Dynamic Core-Attachment). It detects protein-complex core comprising of continually expressed and highly connected proteins in dynamic PPI network, and then the protein complex is formed by including the attachments with high adhesion into the core. The integration of core-attachment feature into the dynamic PPI network is responsible for the superiority of our algorithm. DCA has been applied on two different yeast dynamic PPI networks and the experimental results show that it performs significantly better than the state-of-the-art techniques in terms of prediction accuracy, hF-measure and statistical significance in biology. In addition, the identified complexes with strong biological significance provide potential candidate complexes for biologists to validate.

Safety and core design of large liquid-metal cooled fast breeder reactors

NASA Astrophysics Data System (ADS)

Qvist, Staffan Alexander

In light of the scientific evidence for changes in the climate caused by greenhouse-gas emissions from human activities, the world is in ever more desperate need of new, inexhaustible, safe and clean primary energy sources. A viable solution to this problem is the widespread adoption of nuclear breeder reactor technology. Innovative breeder reactor concepts using liquid-metal coolants such as sodium or lead will be able to utilize the waste produced by the current light water reactor fuel cycle to power the entire world for several centuries to come. Breed & burn (B&B) type fast reactor cores can unlock the energy potential of readily available fertile material such as depleted uranium without the need for chemical reprocessing. Using B&B technology, nuclear waste generation, uranium mining needs and proliferation concerns can be greatly reduced, and after a transitional period, enrichment facilities may no longer be needed. In this dissertation, new passively operating safety systems for fast reactors cores are presented. New analysis and optimization methods for B&B core design have been developed, along with a comprehensive computer code that couples neutronics, thermal-hydraulics and structural mechanics and enables a completely automated and optimized fast reactor core design process. In addition, an experiment that expands the knowledge-base of corrosion issues of lead-based coolants in nuclear reactors was designed and built. The motivation behind the work presented in this thesis is to help facilitate the widespread adoption of safe and efficient fast reactor technology.

Re-refinement of the spliceosomal U4 snRNP core-domain structure

PubMed Central

Li, Jade; Leung, Adelaine K.; Kondo, Yasushi; Oubridge, Chris; Nagai, Kiyoshi

2016-01-01

The core domain of small nuclear ribonucleoprotein (snRNP), comprised of a ring of seven paralogous proteins bound around a single-stranded RNA sequence, functions as the assembly nucleus in the maturation of U1, U2, U4 and U5 spliceosomal snRNPs. The structure of the human U4 snRNP core domain was initially solved at 3.6 Å resolution by experimental phasing using data with tetartohedral twinning. Molecular replacement from this model followed by density modification using untwinned data recently led to a structure of the minimal U1 snRNP at 3.3 Å resolution. With the latter structure providing a search model for molecular replacement, the U4 core-domain structure has now been re-refined. The U4 Sm site-sequence AAUUUUU has been shown to bind to the seven Sm proteins SmF–SmE–SmG–SmD3–SmB–SmD1–SmD2 in an identical manner as the U1 Sm-site sequence AAUUUGU, except in SmD1 where the bound U replaces G. The progression from the initial to the re-refined structure exemplifies a tortuous route to accuracy: where well diffracting crystals of complex assemblies are initially unavailable, the early model errors are rectified by exploiting preliminary interpretations in further experiments involving homologous structures. New insights are obtained from the more accurate model. PMID:26894541

First In-Core Simultaneous Measurements of Nuclear Heating and Thermal Neutron Flux Obtained With the Innovative Mobile Calorimeter CALMOS Inside the OSIRIS Reactor

NASA Astrophysics Data System (ADS)

Carcreff, Hubert; Salmon, Laurent; Bubendorff, Jacques; Lepeltier, Valérie

2016-10-01

Nuclear heating inside a MTR reactor has to be known in order to design and run irradiation experiments which have to fulfill target temperature constraints. This measurement is usually carried out by calorimetry. The innovative calorimetric system, CALMOS, has been studied and built in 2011 for the 70MWth OSIRIS reactor operated by CEA. Thanks to a new type of calorimetric probe, associated to a specific displacement system, it provides measurements along the fissile height and above the core. Calorimeter working modes, measurement procedures, main modeling and experimental results and expected advantages of this new technique have been already presented in previous papers. However, these first in-core measurements were not performed beyond 6 W · g-1, due to an inside temperature limitation imposed by a safety authority requirement. In this paper, we present the first in-core simultaneous measurements of nuclear heating and conventional thermal neutron flux obtained by the CALMOS device at 70 MW nominal reactor power. For the first time, this experimental system was operated in nominal in-core conditions, with nominal neutron flux up to 2.7 1014 n · cm-2 · s-1 and nuclear heating up to 12 W · g-1. After a brief reminder of the calorimetric cell configuration and displacement system specificities, first nuclear heating distributions at nominal power are presented and discussed. In order to reinforce the heating evaluation, a comparison is made between results obtained by the probe calibration coefficient and the zero methods. Thermal neutron flux evaluation from SPND signal processing required a specific TRIPOLI-4 Monte Carlo calculation which has been performed with the precise CALMOS cell geometry. In addition, the Finite Element model for temperatures map prediction inside the calorimetric cell has been upgraded with recent experimental data obtained up to 12 W · g-1. Finally, the experience feedback led us to improvement perspectives. A second device is currently under manufacturing and main technical options are presented.

Research on fission fragment excitation of gases and nuclear pumping of lasers

NASA Technical Reports Server (NTRS)

Schneider, R. T.; Davie, R. N.; Davis, J. F.; Fuller, J. L.; Paternoster, R. R.; Shipman, G. R.; Sterritt, D. E.; Helmick, H. H.

1974-01-01

Experimental investigations of fission fragment excited gases are reported along with a theoretical analysis of population inversions in fission fragment excited helium. Other studies reported include: nuclear augmentation of gas lasers, direct nuclear pumping of a helium-xenon laser, measurements of a repetitively pulsed high-power CO2 laser, thermodynamic properties of UF6 and UF6/He mixtures, and nuclear waste disposal utilizing a gaseous core reactor.

Optimization of 200 MWth and 250 MWt Ship Based Small Long Life NPP

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fitriyani, Dian; Su'ud, Zaki

2010-06-22

Design optimization of ship-based 200 MWth and 250 MWt nuclear power reactors have been performed. The neutronic and thermo-hydraulic programs of the three-dimensional X-Y-Z geometry have been developed for the analysis of ship-based nuclear power plant. Quasi-static approach is adopted to treat seawater effect. The reactor are loop type lead bismuth cooled fast reactor with nitride fuel and with relatively large coolant pipe above reactor core, the heat from primary coolant system is directly transferred to watersteam loop through steam generators. Square core type are selected and optimized. As the optimization result, the core outlet temperature distribution is changing withmore » the elevation angle of the reactor system and the characteristics are discussed.« less

Fabrication and testing of a 4-node micro-pocket fission detector array for the Kansas State University TRIGA Mk. II research nuclear reactor

NASA Astrophysics Data System (ADS)

Reichenberger, Michael A.; Nichols, Daniel M.; Stevenson, Sarah R.; Swope, Tanner M.; Hilger, Caden W.; Unruh, Troy C.; McGregor, Douglas S.; Roberts, Jeremy A.

2017-08-01

Advancements in nuclear reactor core modeling and computational capability have encouraged further development of in-core neutron sensors. Micro-Pocket Fission Detectors (MPFDs) have been fabricated and tested previously, but successful testing of these prior detectors was limited to single-node operation with specialized designs. Described in this work is a modular, four-node MPFD array fabricated and tested at Kansas State University (KSU). The four sensor nodes were equally spaced to span the length of the fuel-region of the KSU TRIGA Mk. II research nuclear reactor core. The encapsulated array was filled with argon gas, serving as an ionization medium in the small cavities of the MPFDs. The unified design improved device ruggedness and simplified construction over previous designs. A 0.315-in. (8-mm) penetration in the upper grid plate of the KSU TRIGA Mk. II research nuclear reactor was used to deploy the array between fuel elements in the core. The MPFD array was coupled to an electronic support system which has been developed to support pulse-mode operation. Neutron-induced pulses were observed on all four sensor channels. Stable device operation was confirmed by testing under steady-state reactor conditions. Each of the four sensors in the array responded to changes in reactor power between 10 kWth and full power (750 kWth). Reactor power transients were observed in real-time including positive transients with periods of 5, 15, and 30 s. Finally, manual reactor power oscillations were observed in real-time.

Gas-cooled nuclear reactor

DOEpatents

Peinado, Charles O.; Koutz, Stanley L.

1985-01-01

A gas-cooled nuclear reactor includes a central core located in the lower portion of a prestressed concrete reactor vessel. Primary coolant gas flows upward through the core and into four overlying heat-exchangers wherein stream is generated. During normal operation, the return flow of coolant is between the core and the vessel sidewall to a pair of motor-driven circulators located at about the bottom of the concrete pressure vessel. The circulators repressurize the gas coolant and return it back to the core through passageways in the underlying core structure. If during emergency conditions the primary circulators are no longer functioning, the decay heat is effectively removed from the core by means of natural convection circulation. The hot gas rising through the core exits the top of the shroud of the heat-exchangers and flows radially outward to the sidewall of the concrete pressure vessel. A metal liner covers the entire inside concrete surfaces of the concrete pressure vessel, and cooling tubes are welded to the exterior or concrete side of the metal liner. The gas coolant is in direct contact with the interior surface of the metal liner and transfers its heat through the metal liner to the liquid coolant flowing through the cooling tubes. The cooler gas is more dense and creates a downward convection flow in the region between the core and the sidewall until it reaches the bottom of the concrete pressure vessel when it flows radially inward and up into the core for another pass. Water is forced to flow through the cooling tubes to absorb heat from the core at a sufficient rate to remove enough of the decay heat created in the core to prevent overheating of the core or the vessel.

CRM1 plays a nuclear role in transporting snoRNPs to nucleoli in higher eukaryotes.

PubMed

Verheggen, Celine; Bertrand, Edouard

2012-03-01

Here, we review the sn- and sno-RNA transport pathways in S. cerevisiae and humans, aiming at understanding how they evolved and how common factors can have distinct functions depending on the RNA they bind. We give a particular emphasis on Tgs1, the cap hypermethylase that is conserved from yeast to humans and appears to play a central role in both sn- and sno-RNA biogenesis. In yeast, Tgs1 hypermethylates sn- and sno-RNAs in the nucleolus. In humans, Tgs1 occurs in two forms: a long isoform (Tgs1 LF), which locates in the cytoplasm and Cajal bodies, which is predominantly associated with snRNAs and a short isoform (Tgs1 SF), which is nuclear and mainly associates with snoRNAs. We show that Tgs1 LF is exported by CRM1 and that interaction with CRM1 competes for binding with the C-terminal domain of the core protein Nop58, which contains the Nucleolar localization signal of Box C/D snoRNPs (NoLS). Our data suggest a model where CRM1 removes Tgs1 LF from snoRNPs, thereby promoting nucleolar targeting via activation of their NoLS. In this review, we argue that CRM1, while first described as an export receptor, can also control the composition of nucleoplasmic complexes. Thus, it could coordinate the fate of these complexes with the general nucleo-cytoplasmic trafficking.

Determination of Irreducible Water Saturation from nuclear magnetic resonance based on fractal theory — a case study of sandstone with complex pore structure

NASA Astrophysics Data System (ADS)

Peng, L.; Pan, H.; Ma, H.; Zhao, P.; Qin, R.; Deng, C.

2017-12-01

The irreducible water saturation (Swir) is a vital parameter for permeability prediction and original oil and gas estimation. However, the complex pore structure of the rocks makes the parameter difficult to be calculated from both laboratory and conventional well logging methods. In this study, an effective statistical method to predict Swir is derived directly from nuclear magnetic resonance (NMR) data based on fractal theory. The spectrum of transversal relaxation time (T2) is normally considered as an indicator of pore size distribution, and the micro- and meso-pore's fractal dimension in two specific range of T2 spectrum distribution are calculated. Based on the analysis of the fractal characteristics of 22 core samples, which were drilled from four boreholes of tight lithologic oil reservoirs of Ordos Basin in China, the positive correlation between Swir and porosity is derived. Afterwards a predicting model for Swir based on linear regressions of fractal dimensions is proposed. It reveals that the Swir is controlled by the pore size and the roughness of the pore. The reliability of this model is tested and an ideal consistency between predicted results and experimental data is found. This model is a reliable supplementary to predict the irreducible water saturation in the case that T2 cutoff value cannot be accurately determined.

Biochemical and proteomic analysis of spliceosome factors interacting with intron-1 of human papillomavirus type-16.

PubMed

Martínez-Salazar, Martha; López-Urrutia, Eduardo; Arechaga-Ocampo, Elena; Bonilla-Moreno, Raul; Martínez-Castillo, Macario; Díaz-Hernández, Job; Del Moral-Hernández, Oscar; Cedillo-Barrón, Leticia; Martines-Juarez, Víctor; De Nova-Ocampo, Monica; Valdes, Jesús; Berumen, Jaime; Villegas-Sepúlveda, Nicolás

2014-12-05

The human papillomavirus type 16 (HPV-16) E6/E7 spliced transcripts are heterogeneously expressed in cervical carcinoma. The heterogeneity of the E6/E7 splicing profile might be in part due to the intrinsic variation of splicing factors in tumor cells. However, the splicing factors that bind the E6/E7 intron 1 (In-1) have not been defined. Therefore, we aimed to identify these factors; we used HeLa nuclear extracts (NE) for in vitro spliceosome assembly. The proteins were allowed to bind to an RNA/DNA hybrid formed by the In-1 transcript and a 5'-biotinylated DNA oligonucleotide complementary to the upstream exon sequence, which prevented interference in protein binding to the intron. The hybrid probes bound with the nuclear proteins were coupled to streptavidin magnetic beads for chromatography affinity purification. Proteins were eluted and identified by mass spectrometry (MS). Approximately 170 proteins were identified by MS, 80% of which were RNA binding proteins, including canonical spliceosome core components, helicases and regulatory splicing factors. The canonical factors were identified as components of the spliceosomal B-complex. Although 35-40 of the identified factors were cognate splicing factors or helicases, they have not been previously detected in spliceosome complexes that were assembled using in vivo or in vitro models. Copyright © 2014 Elsevier B.V. All rights reserved.

Topics in Core-Collapse Supernova Theory: The Formation of Black Holes and the Transport of Neutrinos

NASA Astrophysics Data System (ADS)

O'Connor, Evan Patrick

Core-Collapse Supernovae are one of the most complex astrophysical systems in the universe. They deeply entwine aspects of physics and astrophysics that are rarely side by side in nature. To accurately model core-collapse supernovae one must self-consistently combine general relativity, nuclear physics, neutrino physics, and magneto-hydrodynamics in a symmetry-free computational environment. This is a challenging task, as each one of these aspects on its own is an area of great study. We take an open approach in an effort to encourage collaboration in the core-collapse supernovae community. In this thesis, we develop a new open-source general-relativistic spherically-symmetric Eulerian hydrodynamics code for studying stellar collapse, protoneutron star formation, and evolution until black hole formation. GR1D includes support for finite temperature equations of state and an efficient and qualitatively accurate treatment of neutrino leakage. GR1D implements spherically-symmetric rotation, allowing for the study of slowly rotating stellar collapse. GR1D is available at http://www.stellarcollapse.org. We use GR1D to perform an extensive study of black hole formation in failing core-collapse supernovae. Over 100 presupernova models from various sources are used in over 700 total simulations. We systematically explore the dependence of black hole formation on the input physics: initial zero-age main sequence (ZAMS) mass and metallicity, nuclear equation of state, rotation, and stellar mass loss rates. Assuming the core-collapse supernova mechanism fails and a black hole forms, we find that the outcome, for a given equation of state, can be estimated, to first order, by a single parameter, the compactness of the stellar core at bounce. By comparing the protoneutron star structure at the onset of gravitational instability with solutions of the Tolman-Oppenheimer-Volkof equations, we find that thermal pressure support in the outer protoneutron star core is responsible for raising the maximum protoneutron star mass by up to 25% above the cold neutron star value. By artificially increasing neutrino heating, we find the critical neutrino heating efficiency required for exploding a given progenitor structure and connect these findings with ZAMS conditions. This establishes, albeit approximately, for the first time based on actual collapse simulations, the mapping between ZAMS parameters and the outcome of core collapse. We also use GR1D to study proposed progenitors of long-duration gamma-ray bursts. We find that many of the proposed progenitors have core structures similar to garden-variety core-collapse supernovae. These are not expected to form black holes, a key ingredient of the collapsar model of long-duration gamma-ray bursts. The small fraction of proposed progenitors that are compact enough to form black holes have fast rotating iron cores, making them prone to a magneto-rotational explosion and the formation of a protomagnetar rather than a black hole. Finally, we present preliminary work on a fully general-relativistic neutrino transport code and neutrino-interaction library. Following along with the trends explored in our black hole formation study, we look at the dependence of the neutrino observables on the bounce compactness. We find clear relationships that will allow us to extract details of the core structure from the next galactic supernova. Following the open approach of GR1D, the neutrino transport code will be made open-source upon completion. The open-source neutrino-interaction library, NuLib, is already available at http://www.nulib.org.

The Conceptual Complexity of Vocabulary in Elementary-Grades Core Science Program Textbooks

ERIC Educational Resources Information Center

Fitzgerald, W. Jill; Elmore, Jeff; Kung, Melody; Stenner, A. Jackson

2017-01-01

The researchers explored the conceptual complexity of vocabulary in contemporary elementary-grades core science program textbooks to address two research questions: (1) Can a progression of concepts' complexity level be described across grades? (2) Was there gradual developmental growth of the most complex concepts' networks of associated concepts…

Effects of Oxidation on Oxidation-Resistant Graphite

DOE Office of Scientific and Technical Information (OSTI.GOV)

Windes, William; Smith, Rebecca; Carroll, Mark

2015-05-01

The Advanced Reactor Technology (ART) Graphite Research and Development Program is investigating doped nuclear graphite grades that exhibit oxidation resistance through the formation of protective oxides on the surface of the graphite material. In the unlikely event of an oxygen ingress accident, graphite components within the VHTR core region are anticipated to oxidize so long as the oxygen continues to enter the hot core region and the core temperatures remain above 400°C. For the most serious air-ingress accident which persists over several hours or days the continued oxidation can result in significant structural damage to the core. Reducing the oxidationmore » rate of the graphite core material during any air-ingress accident would mitigate the structural effects and keep the core intact. Previous air oxidation testing of nuclear-grade graphite doped with varying levels of boron-carbide (B4C) at a nominal 739°C was conducted for a limited number of doped specimens demonstrating a dramatic reduction in oxidation rate for the boronated graphite grade. This report summarizes the conclusions from this small scoping study by determining the effects of oxidation on the mechanical strength resulting from oxidation of boronated and unboronated graphite to a 10% mass loss level. While the B4C additive did reduce mechanical strength loss during oxidation, adding B4C dopants to a level of 3.5% or more reduced the as-fabricated compressive strength nearly 50%. This effectively minimized any benefits realized from the protective film formed on the boronated grades. Future work to infuse different graphite grades with silicon- and boron-doped material as a post-machining conditioning step for nuclear components is discussed as a potential solution for these challenges in this report.« less

Design process of the nanofluid injection mechanism in nuclear power plants

NASA Astrophysics Data System (ADS)

Kang, Myoung-Suk; Jee, Changhyun; Park, Sangjun; Bang, In Choel; Heo, Gyunyoung

2011-04-01

Nanofluids, which are engineered suspensions of nanoparticles in a solvent such as water, have been found to show enhanced coolant properties such as higher critical heat flux and surface wettability at modest concentrations, which is a useful characteristic in nuclear power plants (NPPs). This study attempted to provide an example of engineering applications in NPPs using nanofluid technology. From these motivations, the conceptual designs of the emergency core cooling systems (ECCSs) assisted by nanofluid injection mechanism were proposed after following a design framework to develop complex engineering systems. We focused on the analysis of functional requirements for integrating the conventional ECCSs and nanofluid injection mechanism without loss of performance and reliability. Three candidates of nanofluid-engineered ECCS proposed in previous researches were investigated by applying axiomatic design (AD) in the manner of reverse engineering and it enabled to identify the compatibility of functional requirements and potential design vulnerabilities. The methods to enhance such vulnerabilities were referred from TRIZ and concretized for the ECCS of the Korean nuclear power plant. The results show a method to decouple the ECCS designs with the installation of a separate nanofluids injection tank adjacent to the safety injection tanks such that a low pH environment for nanofluids can be maintained at atmospheric pressure which is favorable for their injection in passive manner.

Design process of the nanofluid injection mechanism in nuclear power plants

PubMed Central

2011-01-01

Nanofluids, which are engineered suspensions of nanoparticles in a solvent such as water, have been found to show enhanced coolant properties such as higher critical heat flux and surface wettability at modest concentrations, which is a useful characteristic in nuclear power plants (NPPs). This study attempted to provide an example of engineering applications in NPPs using nanofluid technology. From these motivations, the conceptual designs of the emergency core cooling systems (ECCSs) assisted by nanofluid injection mechanism were proposed after following a design framework to develop complex engineering systems. We focused on the analysis of functional requirements for integrating the conventional ECCSs and nanofluid injection mechanism without loss of performance and reliability. Three candidates of nanofluid-engineered ECCS proposed in previous researches were investigated by applying axiomatic design (AD) in the manner of reverse engineering and it enabled to identify the compatibility of functional requirements and potential design vulnerabilities. The methods to enhance such vulnerabilities were referred from TRIZ and concretized for the ECCS of the Korean nuclear power plant. The results show a method to decouple the ECCS designs with the installation of a separate nanofluids injection tank adjacent to the safety injection tanks such that a low pH environment for nanofluids can be maintained at atmospheric pressure which is favorable for their injection in passive manner. PMID:21711896

Design process of the nanofluid injection mechanism in nuclear power plants.

PubMed

Kang, Myoung-Suk; Jee, Changhyun; Park, Sangjun; Bang, In Choel; Heo, Gyunyoung

2011-04-27

Nanofluids, which are engineered suspensions of nanoparticles in a solvent such as water, have been found to show enhanced coolant properties such as higher critical heat flux and surface wettability at modest concentrations, which is a useful characteristic in nuclear power plants (NPPs). This study attempted to provide an example of engineering applications in NPPs using nanofluid technology. From these motivations, the conceptual designs of the emergency core cooling systems (ECCSs) assisted by nanofluid injection mechanism were proposed after following a design framework to develop complex engineering systems. We focused on the analysis of functional requirements for integrating the conventional ECCSs and nanofluid injection mechanism without loss of performance and reliability. Three candidates of nanofluid-engineered ECCS proposed in previous researches were investigated by applying axiomatic design (AD) in the manner of reverse engineering and it enabled to identify the compatibility of functional requirements and potential design vulnerabilities. The methods to enhance such vulnerabilities were referred from TRIZ and concretized for the ECCS of the Korean nuclear power plant. The results show a method to decouple the ECCS designs with the installation of a separate nanofluids injection tank adjacent to the safety injection tanks such that a low pH environment for nanofluids can be maintained at atmospheric pressure which is favorable for their injection in passive manner.

Abnormal XPD-induced nuclear receptor transactivation in DNA repair disorders: trichothiodystrophy and xeroderma pigmentosum

PubMed Central

Zhou, Xiaolong; Khan, Sikandar G; Tamura, Deborah; Ueda, Takahiro; Boyle, Jennifer; Compe, Emmanuel; Egly, Jean-Marc; DiGiovanna, John J; Kraemer, Kenneth H

2013-01-01

XPD (ERCC2) is a DNA helicase involved in nucleotide excision repair and in transcription as a structural bridge tying the transcription factor IIH (TFIIH) core with the cdk-activating kinase complex, which phosphorylates nuclear receptors. Mutations in XPD are associated with several different phenotypes, including trichothiodystrophy (TTD), with sulfur-deficient brittle hair, bone defects, and developmental abnormalities without skin cancer, xeroderma pigmentosum (XP), with pigmentary abnormalities and increased skin cancer, or XP/TTD with combined features, including skin cancer. We describe the varied clinical features and mutations in nine patients examined at the National Institutes of Health who were compound heterozygotes for XPD mutations but had different clinical phenotypes: four TTD, three XP, and two combined XP/TTD. We studied TFIIH-dependent transactivation by nuclear receptor for vitamin D (VDR) and thyroid in cells from these patients. The vitamin D stimulation ratio of CYP24 and osteopontin was associated with specific pairs of mutations (reduced in 5, elevated in 1) but not correlated with distinct clinical phenotypes. Thyroid receptor stimulation ratio for KLF9 was not significantly different from normal. XPD mutations frequently were associated with abnormal VDR stimulation in compound heterozygote patients with TTD, XP, or XP/TTD. PMID:23232694

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

PubMed

Lee, Yool; Jang, A Reum; Francey, Lauren J; Sehgal, Amita; Hogenesch, John B

2015-08-29

Regulated nuclear translocation of the PER/CRY repressor complex is critical for negative feedback regulation of the circadian clock of mammals. However, the precise molecular mechanism is not fully understood. Here, we report that KPNB1, an importin β component of the ncRNA repressor of nuclear factor of activated T cells (NRON) ribonucleoprotein complex, mediates nuclear translocation and repressor function of the PER/CRY complex. RNAi depletion of KPNB1 traps the PER/CRY complex in the cytoplasm by blocking nuclear entry of PER proteins in human cells. KPNB1 interacts mainly with PER proteins and directs PER/CRY nuclear transport in a circadian fashion. Interestingly, KPNB1 regulates the PER/CRY nuclear entry and repressor function, independently of importin α, its classical partner. Moreover, inducible inhibition of the conserved Drosophila importin β in lateral neurons abolishes behavioral rhythms in flies. Collectively, these data show that KPNB1 is required for timely nuclear import of PER/CRY in the negative feedback regulation of the circadian clock.

A case study for retaining nuclear power experience

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beckjord, E.S.

1996-12-31

Nuclear engineering departments at U.S. universities are rethinking curricula to focus on essentials. Prospective engineers must know nuclear engineering disciplines, but knowing how their engineering forebears solved important problems will empower them even more by learning some history along with engineering. I suggest a way to retain experience, giving an example: the emergency core cooling system (ECCS) controversy and resolution.

First results from GeMS/GSAOI for project SUNBIRD: Supernovae UNmasked By Infra-Red Detection

NASA Astrophysics Data System (ADS)

Kool, E. C.; Ryder, S.; Kankare, E.; Mattila, S.; Reynolds, T.; McDermid, R. M.; Pérez-Torres, M. A.; Herrero-Illana, R.; Schirmer, M.; Efstathiou, A.; Bauer, F. E.; Kotilainen, J.; Väisänen, P.; Baldwin, C.; Romero-Cañizales, C.; Alberdi, A.

2018-02-01

Core collapse supernova (CCSN) rates suffer from large uncertainties as many CCSNe exploding in regions of bright background emission and significant dust extinction remain unobserved. Such a shortfall is particularly prominent in luminous infrared galaxies (LIRGs), which have high star formation (and thus CCSN) rates and host bright and crowded nuclear regions, where large extinctions and reduced search detection efficiency likely lead to a significant fraction of CCSNe remaining undiscovered. We present the first results of project SUNBIRD (Supernovae UNmasked By Infra-Red Detection), where we aim to uncover CCSNe that otherwise would remain hidden in the complex nuclear regions of LIRGs, and in this way improve the constraints on the fraction that is missed by optical seeing-limited surveys. We observe in the near-infrared 2.15 μm Ks-band, which is less affected by dust extinction compared to the optical, using the multiconjugate adaptive optics imager GeMS/GSAOI on Gemini South, allowing us to achieve a spatial resolution that lets us probe close in to the nuclear regions. During our pilot program and subsequent first full year we have discovered three CCSNe and one candidate with projected nuclear offsets as small as 200 pc. When compared to the total sample of LIRG CCSNe discovered in the near-IR and optical, we show that our method is singularly effective in uncovering CCSNe in nuclear regions and we conclude that the majority of CCSNe exploding in LIRGs are not detected as a result of dust obscuration and poor spatial resolution.

The WPI reactor-readying for the next generation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bobek, L.M.

1993-01-01

Built in 1959, the 10-kW open-pool nuclear training reactor at Worcester Polytechnic Institute (WPI) was one of the first such facilities in the nation located on a university campus. Since then, the reactor and its related facilities have been used to train two generations of nuclear engineers and scientists for the nuclear industry. With the use of nuclear technology playing an increasing role in many segments of the economy, WPI with its nuclear reactor facility is committed to continuing its mission of training future nuclear engineers and scientists. The WPI reactor includes a 6-in. beam port, graphite thermal column, andmore » in-core sample facility. The reactor, housed in an open 8000-gal tank of water, is designed so that the core is readily accessible. Both the control console and the peripheral counting equipment used for student projects and laboratory exercises are located in the reactor room. This arrangement provides convenience and flexibility in using the reactor for foil activations in neutron flux measurements, diffusion measurements, radioactive decay measurements, and the neutron activation of samples for analysis. In 1988, the reactor was successfully converted to low-enriched uranium fuel.« less

Roles of nuclear weak rates on the evolution of degenerate cores in stars

NASA Astrophysics Data System (ADS)

Suzuki, Toshio; Tsunodaa, Naofumi; Tsunoda, Yuhsuke; Shimizu, Noritaka; Otsuka, Takaharu

2018-01-01

Electron-capture and β-decay rates in stellar environments are evaluated with the use of new shell-model Hamiltonians for sd-shell and pf-shell nuclei as well as for nuclei belonging to the island of inversion. Important role of the nuclear weak rates on the final evolution of stellar degenerate cores is presented. The weak interaction rates for sd-shell nuclei are calculated to study nuclear Urca processes in O-Ne-Mg cores of stars with 8-10 M⊙ (solar mass) and their effects on the final fate of the stars. Nucleosynthesis of iron-group elements in Type Ia supernova explosions are studied with the weak rates for pf-shell nuclei. The problem of the neutron-rich iron-group isotope over-production compared to the solar abundances is shown to be nearly solved with the use of the new rates and explosion model of slow defraglation with delayed detonation. Evaluation of the weak rates is extended to the island of inversion and the region of neutron-rich nuclei near 78Ni, where two major shells contribute to their configurations.

Minimization of the energy loss of nuclear power plants in case of partial in-core monitoring system failure

NASA Astrophysics Data System (ADS)

Zagrebaev, A. M.; Ramazanov, R. N.; Lunegova, E. A.

2017-01-01

In this paper we consider the optimization problem minimize of the energy loss of nuclear power plants in case of partial in-core monitoring system failure. It is possible to continuation of reactor operation at reduced power or total replacement of the channel neutron measurements, requiring shutdown of the reactor and the stock of detectors. This article examines the reconstruction of the energy release in the core of a nuclear reactor on the basis of the indications of height sensors. The missing measurement information can be reconstructed by mathematical methods, and replacement of the failed sensors can be avoided. It is suggested that a set of ‘natural’ functions determined by means of statistical estimates obtained from archival data be constructed. The procedure proposed makes it possible to reconstruct the field even with a significant loss of measurement information. Improving the accuracy of the restoration of the neutron flux density in partial loss of measurement information to minimize the stock of necessary components and the associated losses.

A new baryonic equation of state at sub-nuclear densities for core-collapse simulations

NASA Astrophysics Data System (ADS)

Furusawa, Shun; Yamada, Shoichi; Sumiyoshi, Kohsuke; Suzuki, Hideyuki

2012-11-01

We construct a new equation of state for baryons at sub-nuclear densities for the use in core-collapse simulations of massive stars. The formulation is based on the nuclear statistical equilibrium description and the liquid drop approximation of nuclei. The model free energy to minimize is calculated by using relativistic mean field theory for nucleons and the mass formula for nuclei with atomic number up to ~ 1000. We have also taken into account the pasta phase. We find that the free energy and other thermodynamical quantities are not very different from those given in the standard EOSs that adopt the single nucleus approximation. On the other hand, the average mass is systematically different, which may have an important effect to the rates of electron captures and coherent neutrino scatterings on nuclei in supernova cores. It is also interesting that the root mean square of the mass number is not very different from the average mass number, since the former is important for the evaluation of coherent scattering rates on nuclei but has been unavailable so far.

Evaluation of external hazards to nuclear power plants in the United States

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kimura, C.Y.; Budnitz, R.J.

1987-12-01

The Lawrence Livermore National Laboratory (LLNL) has performed a study of the risk of core damage to nuclear power plants in the United States due to externally initiated events. The broad objective has been to gain an understanding of whether or not each external initiator is among the major potential accident initiators that may pose a threat of severe reactor core damage or of large radioactive release to the environment from the reactor. Four external hazards were investigated in this report. These external hazards are internal fires, high winds/tornadoes, external floods, and transportation accidents. Analysis was based on two figures-of-merit,more » one based on core damage frequency and the other based on the frequency of large radioactive releases. Using these two figures-of-merit as evaluation criteria, it has been feasible to ascertain whether the risk from externally initiated accidents is, or is not, an important contributor to overall risk for the US nuclear power plants studied. This has been accomplished for each initiator separately. 208 refs., 17 figs., 45 tabs.« less

Possible generation of heat from nuclear fusion in Earth's inner core.

PubMed

Fukuhara, Mikio

2016-11-23

The cause and source of the heat released from Earth's interior have not yet been determined. Some research groups have proposed that the heat is supplied by radioactive decay or by a nuclear georeactor. Here we postulate that the generation of heat is the result of three-body nuclear fusion of deuterons confined in hexagonal FeDx core-centre crystals; the reaction rate is enhanced by the combined attraction effects of high-pressure (~364 GPa) and high-temperature (~5700 K) and by the physical catalysis of neutral pions: 2 D +  2 D +  2 D → 2 1 H +  4 He + 2  + 20.85 MeV. The possible heat generation rate can be calculated as 8.12 × 10 12  J/m 3 , based on the assumption that Earth's primitive heat supply has already been exhausted. The H and He atoms produced and the anti-neutrino are incorporated as Fe-H based alloys in the H-rich portion of inner core, are released from Earth's interior to the universe, and pass through Earth, respectively.

First in-core simultaneous measurements of nuclear heating and thermal neutron flux obtained with the innovative mobile calorimeter CALMOS inside the OSIRIS reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lepeltier, Valerie; Bubendorff, Jacques; Carcreff, Hubert

2015-07-01

Nuclear heating inside a MTR reactor has to be known in order to design and to run irradiation experiments which have to fulfill target temperature constraints. This measurement is usually carried out by calorimetry. The innovative calorimetric system, CALMOS, has been studied and built in 2011 for the 70 MWth OSIRIS reactor operated by CEA. Thanks to a new type of calorimetric probe, associated to a specific displacement system, it provides measurements along the fissile height and above the core. This development required preliminary modelling and irradiation of mock-ups of the calorimetric probe in the ex-core area, where nuclear heatingmore » rate does not exceed 2 W.g{sup -1}. The calorimeter working modes, the different measurement procedures allowed with such a new probe, the main modeling and experimental results and expected advantages of this new technique have been already presented. However, these first in-core measurements were not performed beyond 6 W.g{sup -1}, due to an inside temperature limitation imposed by a safety authority requirement. In this paper, we present the first in-core simultaneous measurements of nuclear heating and conventional thermal neutron flux obtained by the CALMOS device at the 70 MW nominal reactor power. For the first time, this experimental system was operated in nominal in-core conditions, with nominal neutron flux up to 2.7 10{sup 14} n.cm{sup -2}.s{sup -1} and nuclear heating up to 12 W.g{sup -1}. A comprehensive measurement campaign carried out from 2013 to 2015 inside all accessible irradiation locations of the core, allowed to qualify definitively this new device, not only in terms of measurement ability but also in terms of reliability. After a brief reminder of the calorimetric cell configuration and displacement system specificities, first nuclear heating distributions at nominal power are presented and discussed. In order to reinforce the heating evaluation, a systematic comparison is made between results obtained by different methods, the probe calibration coefficient and the zero method. Thermal neutron flux evaluation from the SPND signal processing required a specific TRIPOLI-4 Monte Carlo calculation which has been performed with the precise CALMOS cell geometry. In addition, the Finite Element model for temperatures map prediction inside the calorimetric cell has been upgraded with the recent experimental data obtained up to 12 W.g{sup -1}. The Kc coefficient, taking into account nonlinearities with regard to the calibration, has been reevaluated so as to make relevant measurements up to the nominal reactor power. Finally, the experience feedback acquired until now with this first CALMOS version led us to improvement perspectives. A second device is currently under manufacturing and main technical options chosen for this second version are presented. (authors)« less

Optimize out-of-core thermionic energy conversion for nuclear electric propulsion

NASA Technical Reports Server (NTRS)

Morris, J. F.

1978-01-01

Thermionic energy conversion (TEC) potentialities for nuclear electric propulsion (NEP) are examined. Considering current designs, their limitations, and risks raises critical questions about the use of TEC for NEP. Apparently a reactor cooled by hotter-than-1675 K heat pipes has good potentialities. TEC with higher temperatures and greater power densities than the currently proposed 1650 K, 5-to-6 W/sq cm version offers substantial gains. Other approaches to high-temperature electric isolation appear also promising. A high-power-density, high-temperature TEC for NEP appears, therefore, attainable. It is recommended to optimize out-of-core thermionic energy conversion for nuclear electric propulsion. Although current TEC designs for NEP seem unnecessary compared with Brayton versions, large gains are apparently possible with increased temperatures and greater power densities.

Open cycle gas core nuclear rockets

NASA Technical Reports Server (NTRS)

Ragsdale, Robert

1991-01-01

The open cycle gas core engine is a nuclear propulsion device. Propulsion is provided by hot hydrogen which is heated directly by thermal radiation from the nuclear fuel. Critical mass is sustained in the uranium plasma in the center. It has typically 30 to 50 kg of fuel. It is a thermal reactor in the sense that fissions are caused by absorption of thermal neutrons. The fast neutrons go out to an external moderator/reflector material and, by collision, slow down to thermal energy levels, and then come back in and cause fission. The hydrogen propellant is stored in a tank. The advantage of the concept is very high specific impulse because you can take the plasma to any temperature desired by increasing the fission level by withdrawing or turning control rods or control drums.

The basic route of the nuclear translocation porcine growth hormone (GH)-growth hormone receptor (GHR) complex (pGH/GHR) in porcine hepatocytes.

PubMed

Hainan, Lan; Huilin, Liu; Khan, Mahamad; Xin, Zheng; YuJiang, Yang; Hui, Zhang; Naiquan, Yao

2018-06-08

Traditional views suggest that growth hormone and the growth hormone receptor (GH/GHR complex) exert their functions only on the plasma membrane. This paradigm, however, has been challenged by recent new findings that the GH/GHR complex could translocate into cell nuclei where they could still exhibit important physiological functions. We also reported the nuclear localization of porcine GH/GHR and their potential functions in porcine hepatocytes. However, the basic path of pGH/GHR's nuclear translocation remains unclear. Combining previous research results and our current findings, we proposed two basic routes of pGH/GHR's nuclear transportation as follows: 1) after pGH binding to GHR, pGH/GHR enters into the cytoplasm though clathrin- or caveolin-mediated endocytosis, then the pGH/GHR complex enters into early endosomes (Rab5-positive), and the endosome carries the GH/GHR complex to the endoplasmic reticulum (ER). After endosome docking on the ER, the endosome starts fission, and the pGH/GHR complex enters into the ER lumen. Then the pGH/GHR complex transports into the cytoplasm, possibly by the ERAD pathway. Subsequently, the pGH/GHR complex interacts with IMPα/β, which, in turn, mediates GH/GHR nuclear localization; 2) pGH binds with the GHR on the cell membrane and, subsequently, pGH/GHR internalizes into the cell and enters into the endosome (this endosome may belong to a class of endosomes called envelope-associated endosomes (NAE)). Then, the endosome carries the pGH/GHR to the nuclear membrane. After docking on the nuclear membrane, the pGH/GHR complex fuses with the nuclear membrane and then enters into the cell nucleus. Copyright © 2018 Elsevier Inc. All rights reserved.

Third-generation site characterization: Cryogenic core collection, nuclear magnetic resonance, and electrical resistivity

NASA Astrophysics Data System (ADS)

Kiaalhosseini, Saeed

In modern contaminant hydrology, management of contaminated sites requires a holistic characterization of subsurface conditions. Delineation of contaminant distribution in all phases (i.e., aqueous, non-aqueous liquid, sorbed, and gas), as well as associated biogeochemical processes in a complex heterogeneous subsurface, is central to selecting effective remedies. Arguably, a factor contributing to the lack of success of managing contaminated sites effectively has been the limitations of site characterization methods that rely on monitoring wells and grab sediment samples. The overarching objective of this research is to advance a set of third-generation (3G) site characterization methods to overcome shortcomings of current site characterization techniques. 3G methods include 1) cryogenic core collection (C3) from unconsolidated geological subsurface to improve recovery of sediments and preserving key attributes, 2) high-throughput analysis (HTA) of frozen core in the laboratory to provide high-resolution, depth discrete data of subsurface conditions and processes, 3) resolution of non-aqueous phase liquid (NAPL) distribution within the porous media using a nuclear magnetic resonance (NMR) method, and 4) application of a complex resistivity method to track NAPL depletion in shallow geological formation over time. A series of controlled experiments were conducted to develop the C 3 tools and methods. The critical aspects of C3 are downhole circulation of liquid nitrogen via a cooling system, the strategic use of thermal insulation to focus cooling into the core, and the use of back pressure to optimize cooling. The C3 methods were applied at two contaminated sites: 1) F.E. Warren (FEW) Air Force Base near Cheyenne, WY and 2) a former refinery in the western U.S. The results indicated that the rate of core collection using the C3 methods is on the order of 30 foot/day. The C3 methods also improve core recovery and limits potential biases associated with flowing sands. HTA of frozen core was employed at the former refinery and FEW. Porosity and fluid saturations (i.e., aqueous, non-aqueous liquid, and gas) from the former refinery indicate that given in situ freezing, the results are not biased by drainage of pore fluids from the core during sample collection. At FEW, a comparison between the results of HTA of the frozen core collected in 2014 and the results of site characterization using unfrozen core, (second-generation (2G) methods) at the same locations (performed in 2010) indicate consistently higher contaminant concentrations using C 3. Many factors contribute to the higher quantification of contaminant concentrations using C3. The most significant factor is the preservation of the sediment attributes, in particular, pore fluids and volatile organic compounds (VOCs) in comparison to the unfrozen conventional sediment core. The NMR study was performed on laboratory-fabricated sediment core to resolve NAPL distribution within the porous media qualitatively and quantitatively. The fabricated core consisted of Colorado silica sand saturated with deionized water and trichloroethylene (TCE). The cores were scanned with a BRUKER small-animal scanner (2.3 Tesla, 100 MHz) at 20 °C and while the core was frozen at -25 °C. The acquired images indicated that freezing the water within the core suppressed the NMR signals of water-bound hydrogen. The hydrogen associated with TCE was still detectable since the TCE was in its liquid state (melting point of TCE is -73 °C). Therefore, qualitative detection of TCE within the sediment core was performed via the NMR scanning by freezing the water. A one-dimensional NMR scanning method was used for quantification of TCE mass distribution within the frozen core. However, the results indicated inconsistency in estimating the total TCE mass within the porous media. Downhole NMR logging was performed at the former refinery in the western U.S. to detect NAPL and to discriminate NAPL from water in the formation. The results indicated that detection of NMR signals to discriminate NAPL from water is compromised by the noise stemming from the active facilities and/or power lines passing over the site. A laboratory experiment was performed to evaluate the electrical response of unconsolidated porous media through time (30 days) while NAPL was being depleted. Sand columns (Colorado silica sand) contaminated with methyl tert-butyl ether (MTBE, a light non-aqueous phase liquid (LNAPL)) were studied. A multilevel electrode system was used to measure electrical resistivity of impacted sand by imposing alternative current. The trend of reduction in resistivity through the depth of columns over time followed depletion of LNAPL by volatilization. Finally, a field experiment was performed at the former refinery in the western U.S. to track natural losses of LNAPL over time. Multilevel systems consisting of water samplers, thermocouples, and electrodes were installed at a clean zone (background zone) and an LNAPL-impacted zone. In situ measurements of complex resistivity and temperature were taken and water sampling was performed for each depth (from 3 to 14 feet below the ground surface at one-foot spacing) within almost a year. At both locations, the results indicated decreases in apparent resistivity below the water table over time. This trend was supported by the geochemistry of the pore fluids. Overall, results indicate that application of the electrical resistivity method to track LNAPL depletion at field sites is difficult due to multiple conflicting factors affecting the geoelectrical response of LNAPL-impacted zones over time.

Transmutation of Isotopes --- Ecological and Energy Production Aspects

NASA Astrophysics Data System (ADS)

Gudowski, Waclaw

2000-01-01

This paper describes principles of Accelerator-Driven Transmutation of Nuclear Wastes (ATW) and gives some flavour of the most important topics which are today under investigations in many countries. An assessment of the potential impact of ATW on a future of nuclear energy is also given. Nuclear reactors based on self-sustained fission reactions --- after spectacular development in fifties and sixties, that resulted in deployment of over 400 power reactors --- are wrestling today more with public acceptance than with irresolvable technological problems. In a whole spectrum of reasons which resulted in today's opposition against nuclear power few of them are very relevant for the nuclear physics community and they arose from the fact that development of nuclear power had been handed over to the nuclear engineers and technicians with some generically unresolved problems, which should have been solved properly by nuclear scientists. In a certain degree of simplification one can say, that most of the problems originate from very specific features of a fission phenomenon: self-sustained chain reaction in fissile materials and very strong radioactivity of fission products and very long half-life of some of the fission and activation products. And just this enormous concentration of radioactive fission products in the reactor core is the main problem of managing nuclear reactors: it requires unconditional guarantee for the reactor core integrity in order to avoid radioactive contamination of the environment; it creates problems to handle decay heat in the reactor core and finally it makes handling and/or disposal of spent fuel almost a philosophical issue, due to unimaginable long time scales of radioactive decay of some isotopes. A lot can be done to improve the design of conventional nuclear reactors (like Light Water Reactors); new, better reactors can be designed but it seems today very improbable to expect any radical change in the public perception of conventional nuclear power. In this context a lot of hopes and expectations have been expressed for novel systems called Accelerator-Driven Systems, Accelerator-Driven Transmutation of Waste or just Hybrid Reactors. All these names are used for description of the same nuclear system combining a powerful particle accelerator with a subcritical reactor. A careful analysis of possible environmental impact of ATW together with limitation of this technology is presented also in this paper.

P- T- t constraints on the development of the Doi Inthanon metamorphic core complex domain and implications for the evolution of the western gneiss belt, northern Thailand

NASA Astrophysics Data System (ADS)

Macdonald, A. S.; Barr, S. M.; Miller, B. V.; Reynolds, P. H.; Rhodes, B. P.; Yokart, B.

2010-01-01

The western gneiss belt in northern Thailand is exposed within two overlapping Cenozoic structural domains: the extensional Doi Inthanon metamorphic core complex domain located west of the Chiang Mai basin, and the Mae Ping strike-slip fault domain located west of the Tak batholith. New P- T estimates and U-Pb and 40Ar/ 39Ar age determinations from the Doi Inthanon domain show that the gneiss there records a complex multi-stage history that can be represented by a clockwise P- T- t path. U-Pb zircon and titanite dating of mylonitic calc-silicate gneiss from the Mae Wang area of the complex indicates that the paragneissic sequence experienced high-grade, medium-pressure metamorphism (M1) in the Late Triassic - Early Jurassic (ca. 210 Ma), in good agreement with previously determined zircon ages from the underlying core orthogneiss exposed on Doi Inthanon. Late Cretaceous monazite ages of 84 and 72 Ma reported previously from the core orthogneiss are attributed to a thermal overprint (M2) to upper-amphibolite facies in the sillimanite field. U-Pb zircon and monazite dating of granitic mylonite from the Doi Suthep area of the complex provides an upper age limit of 40 Ma (Late Eocene) for the early stage(s) of development of the actual core complex, by initially ductile, low-angle extensional shearing under lower amphibolite-facies conditions (M3), accompanied by near-isothermal diapiric rise and decompression melting. 40Ar/ 39Ar laserprobe dating of muscovite from both Doi Suthep and Doi Inthanon provided Miocene ages of ca. 26-15 Ma, representing cooling through the ca. 350 °C isotherm and marking late-stage development of the core complex by detachment faulting of the cover rocks and isostatic uplift of the sheared core zone and mantling gneisses in the footwall. Similarities in the thermochronology of high-grade gneisses exposed in the core complex and shear zone domains in the western gneiss belt of northern Thailand (and also in northern Vietnam, Laos, Yunnan, and central Myanmar) suggest a complex regional response to indentation of Southeast Asia by India.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dahabieh, Matthew S., E-mail: dahabieh@interchange.ubc.ca; Ooms, Marcel, E-mail: marcel.ooms@mssm.edu; Malcolm, Tom, E-mail: tmalc1@yahoo.com

Transcription from the HIV-1 long terminal repeat (LTR) is mediated by numerous host transcription factors. In this study we characterized an E-box motif (RBE1) within the core promoter that was previously implicated in both transcriptional activation and repression. We show that RBE1 is a binding site for the RBF-2 transcription factor complex (USF1, USF2, and TFII-I), previously shown to bind an upstream viral element, RBE3. The RBE1 and RBE3 elements formed complexes of identical mobility and protein constituents in gel shift assays, both with Jurkat T-cell nuclear extracts and recombinant USF/TFII-I. Furthermore, both elements are regulators of HIV-1 expression; mutationsmore » in LTR-luciferase reporters and in HIV-1 molecular clones resulted in decreased transcription, virion production, and proviral expression in infected cells. Collectively, our data indicate that RBE1 is a bona fide RBF-2 binding site and that the RBE1 and RBE3 elements are necessary for mediating proper transcription from the HIV-1 LTR.« less

Web-based training course for evaluating radiological dose assessment in NRC's license termination process.

PubMed

Lepoire, D; Richmond, P; Cheng, J-J; Kamboj, S; Arnish, J; Chen, S Y; Barr, C; McKenney, C

2008-08-01

As part of the requirement for terminating the licenses of nuclear power plants or other nuclear facilities, license termination plans or decommissioning plans are submitted by the licensee to the U.S. Nuclear Regulatory Commission (NRC) for review and approval. Decommissioning plans generally refer to the decommissioning of nonreactor facilities, while license termination plans specifically refer to the decommissioning of nuclear reactor facilities. To provide a uniform and consistent review of dose modeling aspects of these plans and to address NRC-wide knowledge management issues, the NRC, in 2006, commissioned Argonne National Laboratory to develop a Web-based training course on reviewing radiological dose assessments for license termination. The course, which had first been developed in 2005 to target specific aspects of the review processes for license termination plans and decommissioning plans, evolved from a live classroom course into a Web-based training course in 2006. The objective of the Web-based training course is to train NRC staff members (who have various relevant job functions and are located at headquarters, regional offices, and site locations) to conduct an effective review of dose modeling in accordance with the latest NRC guidance, including NUREG-1757, Volumes 1 and 2. The exact size of the staff population who will receive the training has not yet been accurately determined but will depend on various factors such as the decommissioning activities at the NRC. This Web-based training course is designed to give NRC staff members modern, flexible access to training. To this end, the course is divided into 16 modules: 9 core modules that deal with basic topics, and 7 advanced modules that deal with complex issues or job-specific topics. The core and advanced modules are tailored to various NRC staff members with different job functions. The Web-based system uses the commercially available software Articulate, which incorporates audio, video, and animation in slide presentations and has glossary, document search, and Internet connectivity features. The training course has been implemented on an NRC system that allows staff members to register, select courses, track records, and self-administer quizzes.

Radio-Frequency Driven Dielectric Heaters for Non-Nuclear Testing in Nuclear Core Development

NASA Technical Reports Server (NTRS)

Sims, William Herbert, III (Inventor); Godfroy, Thomas J. (Inventor); Bitteker, Leo (Inventor)

2006-01-01

Apparatus and methods are provided through which a radiofrequency dielectric heater has a cylindrical form factor, a variable thermal energy deposition through variations in geometry and composition of a dielectric, and/or has a thermally isolated power input.

Rift Valley fever phlebovirus NSs protein core domain structure suggests molecular basis for nuclear filaments

PubMed Central

Miller, Ona K; Potter, Jane A; Vijayakrishnan, Swetha; Bhella, David; Naismith, James H; Elliott, Richard M

2017-01-01

Rift Valley fever phlebovirus (RVFV) is a clinically and economically important pathogen increasingly likely to cause widespread epidemics. RVFV virulence depends on the interferon antagonist non-structural protein (NSs), which remains poorly characterized. We identified a stable core domain of RVFV NSs (residues 83–248), and solved its crystal structure, a novel all-helical fold organized into highly ordered fibrils. A hallmark of RVFV pathology is NSs filament formation in infected cell nuclei. Recombinant virus encoding the NSs core domain induced intranuclear filaments, suggesting it contains all essential determinants for nuclear translocation and filament formation. Mutations of key crystal fibril interface residues in viruses encoding full-length NSs completely abrogated intranuclear filament formation in infected cells. We propose the fibrillar arrangement of the NSs core domain in crystals reveals the molecular basis of assembly of this key virulence factor in cell nuclei. Our findings have important implications for fundamental understanding of RVFV virulence. PMID:28915104

Rift Valley fever phlebovirus NSs protein core domain structure suggests molecular basis for nuclear filaments.

PubMed

Barski, Michal; Brennan, Benjamin; Miller, Ona K; Potter, Jane A; Vijayakrishnan, Swetha; Bhella, David; Naismith, James H; Elliott, Richard M; Schwarz-Linek, Ulrich

2017-09-15

Rift Valley fever phlebovirus (RVFV) is a clinically and economically important pathogen increasingly likely to cause widespread epidemics. RVFV virulence depends on the interferon antagonist non-structural protein (NSs), which remains poorly characterized. We identified a stable core domain of RVFV NSs (residues 83-248), and solved its crystal structure, a novel all-helical fold organized into highly ordered fibrils. A hallmark of RVFV pathology is NSs filament formation in infected cell nuclei. Recombinant virus encoding the NSs core domain induced intranuclear filaments, suggesting it contains all essential determinants for nuclear translocation and filament formation. Mutations of key crystal fibril interface residues in viruses encoding full-length NSs completely abrogated intranuclear filament formation in infected cells. We propose the fibrillar arrangement of the NSs core domain in crystals reveals the molecular basis of assembly of this key virulence factor in cell nuclei. Our findings have important implications for fundamental understanding of RVFV virulence.

Design Study of Modular Nuclear Power Plant with Small Long Life Gas Cooled Fast Reactors Utilizing MOX Fuel

NASA Astrophysics Data System (ADS)

Ilham, Muhammad; Su'ud, Zaki

2017-01-01

Growing energy needed due to increasing of the world’s population encourages development of technology and science of nuclear power plant in its safety and security. In this research, it will be explained about design study of modular fast reactor with helium gas cooling (GCFR) small long life reactor, which can be operated over 20 years. It had been conducted about neutronic design GCFR with Mixed Oxide (UO2-PuO2) fuel in range of 100-200 MWth NPPs of power and 50-60% of fuel fraction variation with cylindrical pin cell and cylindrical balance of reactor core geometry. Calculation method used SRAC-CITATION code. The obtained results are the effective multiplication factor and density value of core reactor power (with geometry optimalization) to obtain optimum design core reactor power, whereas the obtained of optimum core reactor power is 200 MWth with 55% of fuel fraction and 9-13% of percentages.

Scale Model Test and Transient Analysis of Steam Injector Driven Passive Core Injection System for Innovative-Simplified Nuclear Power Plant

NASA Astrophysics Data System (ADS)

Ohmori, Shuichi; Narabayashi, Tadashi; Mori, Michitsugu

A steam injector (SI) is a simple, compact and passive pump and also acts as a high-performance direct-contact compact heater. This provides SI with capability to serve also as a direct-contact feed-water heater that heats up feed-water by using extracted steam from turbine. Our technology development aims to significantly simplify equipment and reduce physical quantities by applying "high-efficiency SI", which are applicable to a wide range of operation regimes beyond the performance and applicable range of existing SIs and enables unprecedented multistage and parallel operation, to the low-pressure feed-water heaters and emergency core cooling system of nuclear power plants, as well as achieve high inherent safety to prevent severe accidents by keeping the core covered with water (a severe accident-free concept). This paper describes the results of the scale model test, and the transient analysis of SI-driven passive core injection system (PCIS).

The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles

PubMed Central

Topalidou, Irini; Cattin-Ortolá, Jérôme; MacCoss, Michael J.

2016-01-01

The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP) complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment. PMID:27191843

Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture

PubMed Central

Ding, Zhao-Ying; Huang, Yu-Cheng; Lee, Myong-Chol; Tseng, Min-Jen; Chi, Ya-Hui

2017-01-01

Linker of nucleoskeleton and cytoskeleton (LINC) complexes spanning the nuclear envelope (NE) contribute to nucleocytoskeletal force transduction. A few NE proteins have been found to regulate the LINC complex. In this study, we identify one, Kuduk (Kud), which can reside at the outer nuclear membrane and is required for the development of Drosophila melanogaster ovarian follicles and NE morphology of myonuclei. Kud associates with LINC complex components in an evolutionarily conserved manner. Loss of Kud increases the level but impairs functioning of the LINC complex. Overexpression of Kud suppresses NE targeting of cytoskeleton-free LINC complexes. Thus, Kud acts as a quality control mechanism for LINC-mediated nucleocytoskeletal connections. Genetic data indicate that Kud also functions independently of the LINC complex. Overexpression of the human orthologue TMEM258 in Drosophila proved functional conservation. These findings expand our understanding of the regulation of LINC complexes and NE architecture. PMID:28716842

The origin of the mid-infrared nuclear polarization of active galactic nuclei

NASA Astrophysics Data System (ADS)

Lopez-Rodriguez, E.; Alonso-Herrero, A.; Diaz-Santos, T.; Gonzalez-Martin, O.; Ichikawa, K.; Levenson, N. A.; Martinez-Paredes, M.; Nikutta, R.; Packham, C.; Perlman, E.; Almeida, C. Ramos; Rodriguez-Espinosa, J. M.; Telesco, C. M.

2018-05-01

We combine new (NGC 1275, NGC 4151, and NGC 5506) and previously published (Cygnus A, Mrk 231, and NGC 1068) sub-arcsecond resolution mid-infrared (MIR; 8-13 μm) imaging- and spectro-polarimetric observations of six Seyfert galaxies using CanariCam on the 10.4-m Gran Telescopio CANARIAS. These observations reveal a diverse set of physical processes responsible for the nuclear polarization, and permit characterization of the origin of the MIR nuclear polarimetric signature of active galactic nuclei (AGN). For all radio quiet objects, we found that the nuclear polarization is low (<1 per cent), and the degree of polarization is often a few per cent over extended regions of the host galaxy where we have sensitivity to detect such extended emission (i.e., NGC 1068 and NGC 4151). We suggest that the higher degree of polarization previously found in lower resolution data arises only on the larger-than-nuclear scales. Only the radio-loud Cygnus A exhibits significant nuclear polarization (˜11 per cent), attributable to synchrotron emission from the pc-scale jet close to the core. We present polarization models that suggest that the MIR nuclear polarization for highly obscured objects arises from a self-absorbed MIR polarized clumpy torus and/or dichroism from the host galaxy, while for unabsorbed cores, MIR polarization arises from dust scattering in the torus and/or surrounding nuclear dust.

Impact of the High Flux Isotope Reactor HEU to LEU Fuel Conversion on Cold Source Nuclear Heat Generation Rates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chandler, David

2014-03-01

Under the sponsorship of the US Department of Energy National Nuclear Security Administration, staff members at the Oak Ridge National Laboratory have been conducting studies to determine whether the High Flux Isotope Reactor (HFIR) can be converted from high enriched uranium (HEU) fuel to low enriched uranium (LEU) fuel. As part of these ongoing studies, an assessment of the impact that the HEU to LEU fuel conversion has on the nuclear heat generation rates in regions of the HFIR cold source system and its moderator vessel was performed and is documented in this report. Silicon production rates in the coldmore » source aluminum regions and few-group neutron fluxes in the cold source moderator were also estimated. Neutronics calculations were performed with the Monte Carlo N-Particle code to determine the nuclear heat generation rates in regions of the HFIR cold source and its vessel for the HEU core operating at a full reactor power (FP) of 85 MW(t) and the reference LEU core operating at an FP of 100 MW(t). Calculations were performed with beginning-of-cycle (BOC) and end-of-cycle (EOC) conditions to bound typical irradiation conditions. Average specific BOC heat generation rates of 12.76 and 12.92 W/g, respectively, were calculated for the hemispherical region of the cold source liquid hydrogen (LH2) for the HEU and LEU cores, and EOC heat generation rates of 13.25 and 12.86 W/g, respectively, were calculated for the HEU and LEU cores. Thus, the greatest heat generation rates were calculated for the EOC HEU core, and it is concluded that the conversion from HEU to LEU fuel and the resulting increase of FP from 85 MW to 100 MW will not impact the ability of the heat removal equipment to remove the heat deposited in the cold source system. Silicon production rates in the cold source aluminum regions are estimated to be about 12.0% greater at BOC and 2.7% greater at EOC for the LEU core in comparison to the HEU core. Silicon is aluminum s major transmutation product and affects mechanical properties of aluminum including density, neutron irradiation hardening, swelling, and loss of ductility. Because slightly greater quantities of silicon will be produced in the cold source moderator vessel for the LEU core, these effects will be slightly greater for the LEU core than for the HEU core. Three-group (thermal, epithermal, and fast) neutron flux results tallied in the cold source LH2 hemisphere show greater values for the LEU core under both BOC and EOC conditions. The thermal neutron flux in the LH2 hemisphere for the LEU core is about 12.4% greater at BOC and 2.7% greater at EOC than for the HEU core. Therefore, cold neutron scattering will not be adversely affected and the 4 12 neutrons conveyed to the cold neutron guide hall for research applications will be enhanced.« less

Innovative nuclear thermal propulsion technology evaluation - Results of the NASA/DOE task team study

NASA Technical Reports Server (NTRS)

Howe, Steven D.; Borowski, Stanley; Motloch, Chet; Helms, Ira; Diaz, Nils; Anghaie, Samim; Latham, Thomas

1991-01-01

In response to findings from two NASA/DOE nuclear propulsion workshops, six task teams were created to continue evaluation of various propulsion concepts, from which evolved an innovative concepts subpanel to evaluate thermal propulsion concepts which did not utilize solid fuel. This subpanel endeavored to evaluate each concept on a level technology basis, and to identify critical issues, technologies, and early proof-of-concept experiments. Results of the concept studies including the liquid core fission, the gas core fission, the fission foil reactors, explosively driven systems, fusion, and antimatter are presented.

Satellite nuclear power station: An engineering analysis

NASA Technical Reports Server (NTRS)

Williams, J. R.; Clement, J. D.; Rosa, R. J.; Kirby, K. D.; Yang, Y. Y.

1973-01-01

A nuclear-MHD power plant system which uses a compact non-breeder reactor to produce power in the multimegawatt range is analyzed. It is shown that, operated in synchronous orbit, the plant would transmit power safely to the ground by a microwave beam. Fuel reprocessing would take place in space, and no radioactive material would be returned to earth. Even the effect of a disastrous accident would have negligible effect on earth. A hydrogen moderated gas core reactor, or a colloid-core, or NERVA type reactor could also be used. The system is shown to approach closely the ideal of economical power without pollution.

BOLD VENTURE COMPUTATION SYSTEM for nuclear reactor core analysis, Version III

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vondy, D.R.; Fowler, T.B.; Cunningham, G.W. III.

1981-06-01

This report is a condensed documentation for VERSION III of the BOLD VENTURE COMPUTATION SYSTEM for nuclear reactor core analysis. An experienced analyst should be able to use this system routinely for solving problems by referring to this document. Individual reports must be referenced for details. This report covers basic input instructions and describes recent extensions to the modules as well as to the interface data file specifications. Some application considerations are discussed and an elaborate sample problem is used as an instruction aid. Instructions for creating the system on IBM computers are also given.

Native structure of a type IV secretion system core complex essential for Legionella pathogenesis.

PubMed

Kubori, Tomoko; Koike, Masafumi; Bui, Xuan Thanh; Higaki, Saori; Aizawa, Shin-Ichi; Nagai, Hiroki

2014-08-12

Bacterial type IV secretion systems are evolutionarily related to conjugation systems and play a pivotal role in infection by delivering numerous virulence factors into host cells. Using transmission electron microscopy, we report the native molecular structure of the core complex of the Dot/Icm type IV secretion system encoded by Legionella pneumophila, an intracellular human pathogen. The biochemically isolated core complex, composed of at least five proteins--DotC, DotD, DotF, DotG, and DotH--has a ring-shaped structure. Intriguingly, morphologically distinct premature complexes are formed in the absence of DotG or DotF. Our data suggest that DotG forms a central channel spanning inner and outer membranes. DotF, a component dispensable for type IV secretion, plays a role in efficient embedment of DotG into the functional core complex. These results highlight a common scheme for the biogenesis of transport machinery.

Rapid screening for nuclear genes mutations in isolated respiratory chain complex I defects.

PubMed

Pagniez-Mammeri, Hélène; Lombes, Anne; Brivet, Michèle; Ogier-de Baulny, Hélène; Landrieu, Pierre; Legrand, Alain; Slama, Abdelhamid

2009-04-01

Complex I or reduced nicotinamide adenine dinucleotide (NADH): ubiquinone oxydoreductase deficiency is the most common cause of respiratory chain defects. Molecular bases of complex I deficiencies are rarely identified because of the dual genetic origin of this multi-enzymatic complex (nuclear DNA and mitochondrial DNA) and the lack of phenotype-genotype correlation. We used a rapid method to screen patients with isolated complex I deficiencies for nuclear genes mutations by Surveyor nuclease digestion of cDNAs. Eight complex I nuclear genes, among the most frequently mutated (NDUFS1, NDUFS2, NDUFS3, NDUFS4, NDUFS7, NDUFS8, NDUFV1 and NDUFV2), were studied in 22 cDNA fragments spanning their coding sequences in 8 patients with a biochemically proved complex I deficiency. Single nucleotide polymorphisms and missense mutations were detected in 18.7% of the cDNA fragments by Surveyor nuclease treatment. Molecular defects were detected in 3 patients. Surveyor nuclease screening is a reliable method for genotyping nuclear complex I deficiencies, easy to interpret, and limits the number of sequence reactions. Its use will enhance the possibility of prenatal diagnosis and help us for a better understanding of complex I molecular defects.

The Common Core State Standards' Quantitative Text Complexity Trajectory: Figuring out How Much Complexity Is Enough

ERIC Educational Resources Information Center

Williamson, Gary L.; Fitzgerald, Jill; Stenner, A. Jackson

2013-01-01

The Common Core State Standards (CCSS) set a controversial aspirational, quantitative trajectory for text complexity exposure for readers throughout the grades, aiming for all high school graduates to be able to independently read complex college and workplace texts. However, the trajectory standard is presented without reference to how the…

Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway.

PubMed

Jiang, Xiao-Hua; Xie, Yu-Tao; Cai, Ya-Ping; Ren, Jing; Ma, Tao

2017-05-25

Hepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in various liver cells, and explored the molecular mechanism underlying HCV-related hepatocarcinogenesis. Compared with the empty vector control, HCV core protein and NS4B demonstrated the following characteristics in the Huh7 cells: significantly enhanced β-catenin/Tcf-dependent transcriptional activity (F = 40.87, P < 0.01); increased nuclear translocation of β-catenin (F = 165.26, P < 0.01); upregulated nuclear β-catenin, cytoplasmic β-catenin, Wnt1, c-myc, and cyclin D1 protein expression (P < 0.01); and promoted proliferation of Huh7 cells (P < 0.01 or P < 0.05). Neither protein enhanced β-catenin/Tcf-dependent transcriptional activity in the LO2 cells (F = 0.65, P > 0.05), but they did significantly enhance Wnt3a-induced β-catenin/Tcf-dependent transcriptional activity (F = 64.25, P < 0.01), and promoted the nuclear translocation of β-catenin (F = 66.54, P < 0.01) and the Wnt3a-induced proliferation of LO2 cells (P < 0.01 or P < 0.05). Moreover, activation of the Wnt/β-catenin signaling pathway was greater with the core protein than with NS4B (P < 0.01 or P < 0.05). HCV core protein and NS4B directly activate the Wnt/β-catenin signaling pathway in Huh7 cells and LO2 cells induced by Wnt3a. These data suggest that HCV core protein and NS4B contribute to HCV-associated hepatocellular carcinogenesis.

AGC-2 Irradiation Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rohrbaugh, David Thomas; Windes, William; Swank, W. David

The Next Generation Nuclear Plant (NGNP) will be a helium-cooled, very high temperature reactor (VHTR) with a large graphite core. In past applications, graphite has been used effectively as a structural and moderator material in both research and commercial high temperature gas cooled reactor (HTGR) designs.[ , ] Nuclear graphite H 451, used previously in the United States for nuclear reactor graphite components, is no longer available. New nuclear graphites have been developed and are considered suitable candidates for the new NGNP reactor design. To support the design and licensing of NGNP core components within a commercial reactor, a completemore » properties database must be developed for these current grades of graphite. Quantitative data on in service material performance are required for the physical, mechanical, and thermal properties of each graphite grade with a specific emphasis on data related to the life limiting effects of irradiation creep on key physical properties of the NGNP candidate graphites. Based on experience with previous graphite core components, the phenomenon of irradiation induced creep within the graphite has been shown to be critical to the total useful lifetime of graphite components. Irradiation induced creep occurs under the simultaneous application of high temperatures, neutron irradiation, and applied stresses within the graphite components. Significant internal stresses within the graphite components can result from a second phenomenon—irradiation induced dimensional change. In this case, the graphite physically changes i.e., first shrinking and then expanding with increasing neutron dose. This disparity in material volume change can induce significant internal stresses within graphite components. Irradiation induced creep relaxes these large internal stresses, thus reducing the risk of crack formation and component failure. Obviously, higher irradiation creep levels tend to relieve more internal stress, thus allowing the components longer useful lifetimes within the core. Determining the irradiation creep rates of nuclear grade graphites is critical for determining the useful lifetime of graphite components and is a major component of the Advanced Graphite Creep (AGC) experiment.« less

Integrally cored ceramic investment casting mold fabricated by ceramic stereolithography

NASA Astrophysics Data System (ADS)

Bae, Chang-Jun

Superalloy airfoils are produced by investment casting (IC), which uses ceramic cores and wax patterns with ceramic shell molds. Hollow cored superalloy airfoils in a gas turbine engine are an example of complex IC parts. The complex internal hollow cavities of the airfoil are designed to conduct cooling air through one or more passageways. These complex internal passageways have been fabricated by a lost wax process requiring several processing steps; core preparation, injection molding for wax pattern, and dipping process for ceramic shell molds. Several steps generate problems such as high cost and decreased accuracy of the ceramic mold. For example, costly tooling and production delay are required to produce mold dies for complex cores and wax patterns used in injection molding, resulting in a big obstacle for prototypes and smaller production runs. Rather than using separate cores, patterns, and shell molds, it would be advantageous to directly produce a mold that has the casting cavity and the ceramic core by one process. Ceramic stereolithography (CerSLA) can be used to directly fabricate the integrally cored ceramic casting mold (ICCM). CerSLA builds ceramic green objects from CAD files from many thin liquid layers of powder in monomer, which are solidified by polymerization with a UV laser, thereby "writing" the design for each slice. This dissertation addresses the integrally cored casting ceramic mold (ICCM), the ceramic core with a ceramic mold shell in a single patternless construction, fabricated by ceramic stereolithography (CerSLA). CerSLA is considered as an alternative method to replace lost wax processes, for small production runs or designs too complex for conventional cores and patterns. The main topic is the development of methods to successfully fabricate an ICCM by CerSLA from refractory silica, as well as related issues. The related issues are the segregation of coarse fused silica powders in a layer, the degree of segregation parameter to prevent segregation, and sintering and cristobalite transformation in fused silica compacts.

Radial blanket assembly orificing arrangement

DOEpatents

Patterson, J.F.

1975-07-01

A nuclear reactor core for a liquid metal cooled fast breeder reactor is described in which means are provided for increasing the coolant flow through the reactor fuel assemblies as the reactor ages by varying the coolant flow rate with the changing coolant requirements during the core operating lifetime. (auth)

Nuclear equation of state for core-collapse supernova simulations with realistic nuclear forces

NASA Astrophysics Data System (ADS)

Togashi, H.; Nakazato, K.; Takehara, Y.; Yamamuro, S.; Suzuki, H.; Takano, M.

2017-05-01

A new table of the nuclear equation of state (EOS) based on realistic nuclear potentials is constructed for core-collapse supernova numerical simulations. Adopting the EOS of uniform nuclear matter constructed by two of the present authors with the cluster variational method starting from the Argonne v18 and Urbana IX nuclear potentials, the Thomas-Fermi calculation is performed to obtain the minimized free energy of a Wigner-Seitz cell in non-uniform nuclear matter. As a preparation for the Thomas-Fermi calculation, the EOS of uniform nuclear matter is modified so as to remove the effects of deuteron cluster formation in uniform matter at low densities. Mixing of alpha particles is also taken into account following the procedure used by Shen et al. (1998, 2011). The critical densities with respect to the phase transition from non-uniform to uniform phase with the present EOS are slightly higher than those with the Shen EOS at small proton fractions. The critical temperature with respect to the liquid-gas phase transition decreases with the proton fraction in a more gradual manner than in the Shen EOS. Furthermore, the mass and proton numbers of nuclides appearing in non-uniform nuclear matter with small proton fractions are larger than those of the Shen EOS. These results are consequences of the fact that the density derivative coefficient of the symmetry energy of our EOS is smaller than that of the Shen EOS.

Foundations of Nuclear Geophysics

NASA Astrophysics Data System (ADS)

Herndon, J. M.; Hollenbach, D. F.

2002-05-01

Herndon suggested that the inner core of the Earth consists, not of partially crystallized iron metal, but of nickel silicide. He has shown by fundamental mass ratios that i) the Earth as a whole, especially the inner 82%, has a state of oxidation like primitive enstatite chondrites, and ii) the lower mantle and core are similar in composition to the Abee enstatite chondrite. By analogy with Abee data, CaS and MgS precipitates from the core are expected to collect at the core-mantle boundary and, significantly, a major fraction of the actinides are expected to precipitate from the core and to collect at the center of the Earth. Herndon demonstrated the feasibility of a nuclear fission reactor at the center of the Earth as the energy source for the geomagnetic field and described a natural mechanism that would lead to variations in energy production and thus variations in the geomagnetic field. Hollenbach and Herndon produced numerical simulations of the operation of the geo-reactor over the lifetime of the Earth using the state-of-the-art, validated, industry standard SCALE code package developed at Oak Ridge National Laboratory. The results clearly demonstrate that such a geo-reactor would i) function as a fast-neutron breeder reactor; ii) under appropriate conditions, operate over the entire period of geologic time; iii) function in such a manner as to yield variable and/or intermittent output; iv) generate energy at levels in the range generally accepted by the geophysics community; and, v) produce He-3 and He-4 in ratios that are in the range observed from deep-mantle sources. Deep-source He-3, the authors submit, is evidence of in-core sustained nuclear fission, rather than the out-gassing of primordial He-3; which in turn is evidence of large amounts of uranium residing in the Earth's core; which in turn is evidence that the core has a state of oxidation like the corresponding matter in primitive enstatite chondrites. The factors affecting He-3/He-4 ratios, their causes and implications, will be discussed in the presentation. Also, the current state of investigations into additional deep-Earth nuclear fission signatures will be presented. References: J. M. Herndon, Proc. R. Roc. London, Ser. A, 368 (1979) 495; J. Geomagn. Geoelectr. 45 (1993) 423; Proc. R. Soc. London, Ser. A, 445 (1994) 453; Proc. Nat. Acad. Sci. (USA) 93 (1996) 646. Hollenbach, D. F. and J. M. Herndon, Proc. Nat. Acad. Sci. (USA) 98 (2001) 11085.

Equation of state for neutron stars. Some recent developments

NASA Astrophysics Data System (ADS)

Haensel, P.; Fortin, M.

2017-12-01

Calculations using the chiral effective field theory (ChEFT) indicate that the four-body force contribution to the equation of state (EOS) of pure neutron matter (PNM) at the nuclear density n 0 is negligibly small. However, the overall uncertainty in the EOS of PNM at n 0 remains ∼ 20%. Relativistic mean field (RMF) calculations with in-medium scaling, and including hyperons and Δ resonances, can be made consistent with recent nuclear and astrophysical constraints. Dirac-Brueckner-Hartree-Fock calculations with some medium dependence of the nuclear interaction yield neutron star (NS) models with hyperonic cores consistent with 2 M⊙ stars and agreeing with the saturation parameters of nuclear matter. Many unified EOS for the NS crust and core were calculated, and are reviewed here. The effect of the finite size of baryons on the EOS, its treatment via the excluded-volume approximation, and its relevance for the hypothetical hybrid-star twins at ∼ 2 M⊙ are dicussed.

End-to-End Demonstrator of the Safe Affordable Fission Engine (SAFE) 30: Power Conversion and Ion Engine Operation

NASA Technical Reports Server (NTRS)

Hrbud, Ivana; VanDyke, Melissa; Houts, Mike; Goodfellow, Keith; Schafer, Charles (Technical Monitor)

2001-01-01

The Safe Affordable Fission Engine (SAFE) test series addresses Phase 1 Space Fission Systems issues in particular non-nuclear testing and system integration issues leading to the testing and non-nuclear demonstration of a 400-kW fully integrated flight unit. The first part of the SAFE 30 test series demonstrated operation of the simulated nuclear core and heat pipe system. Experimental data acquired in a number of different test scenarios will validate existing computational models, demonstrated system flexibility (fast start-ups, multiple start-ups/shut downs), simulate predictable failure modes and operating environments. The objective of the second part is to demonstrate an integrated propulsion system consisting of a core, conversion system and a thruster where the system converts thermal heat into jet power. This end-to-end system demonstration sets a precedent for ground testing of nuclear electric propulsion systems. The paper describes the SAFE 30 end-to-end system demonstration and its subsystems.

Benchmark gas core critical experiment.

NASA Technical Reports Server (NTRS)

Kunze, J. F.; Lofthouse, J. H.; Cooper, C. G.; Hyland, R. E.

1972-01-01

A critical experiment with spherical symmetry has been conducted on the gas core nuclear reactor concept. The nonspherical perturbations in the experiment were evaluated experimentally and produce corrections to the observed eigenvalue of approximately 1% delta k. The reactor consisted of a low density, central uranium hexafluoride gaseous core, surrounded by an annulus of void or low density hydrocarbon, which in turn was surrounded with a 97-cm-thick heavy water reflector.

Minimizing or eliminating refueling of nuclear reactor

DOEpatents

Doncals, Richard A.; Paik, Nam-Chin; Andre, Sandra V.; Porter, Charles A.; Rathbun, Roy W.; Schwallie, Ambrose L.; Petras, Diane S.

1989-01-01

Demand for refueling of a liquid metal fast nuclear reactor having a life of 30 years is eliminated or reduced to intervals of at least 10 years by operating the reactor at a low linear-power density, typically 2.5 kw/ft of fuel rod, rather than 7.5 or 15 kw/ft, which is the prior art practice. So that power of the same magnitude as for prior art reactors is produced, the volume of the core is increased. In addition, the height of the core and it diameter are dimensioned so that the ratio of the height to the diameter approximates 1 to the extent practicable considering the requirement of control and that the pressure drop in the coolant shall not be excessive. The surface area of a cylinder of given volume is a minimum if the ratio of the height to the diameter is 1. By minimizing the surface area, the leakage of neutrons is reduced. By reducing the linear-power density, increasing core volume, reducing fissile enrichment and optimizing core geometry, internal-core breeding of fissionable fuel is substantially enhanced. As a result, core operational life, limited by control worth requirements and fuel burnup capability, is extended up to 30 years of continuous power operation.

Thermal margin protection system for a nuclear reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Musick, C.R.

1974-02-12

A thermal margin protection system for a nuclear reactor is described where the coolant flow flow trip point and the calculated thermal margin trip point are switched simultaneously and the thermal limit locus is made more restrictive as the allowable flow rate is decreased. The invention is characterized by calculation of the thermal limit Locus in response to applied signals which accurately represent reactor cold leg temperature and core power; cold leg temperature being corrected for stratification before being utilized and reactor power signals commensurate with power as a function of measured neutron flux and thermal energy added to themore » coolant being auctioneered to select the more conservative measure of power. The invention further comprises the compensation of the selected core power signal for the effects of core radial peaking factor under maximum coolant flow conditions. (Official Oazette)« less

Heat pipe nuclear reactor for space power

NASA Technical Reports Server (NTRS)

Koening, D. R.

1976-01-01

A heat-pipe-cooled nuclear reactor has been designed to provide 3.2 MWth to an out-of-core thermionic conversion system. The reactor is a fast reactor designed to operate at a nominal heat-pipe temperature of 1675 K. Each reactor fuel element consists of a hexagonal molybdenum block which is bonded along its axis to one end of a molybdenum/lithium-vapor heat pipe. The block is perforated with an array of longitudinal holes which are loaded with UO2 pellets. The heat pipe transfers heat directly to a string of six thermionic converters which are bonded along the other end of the heat pipe. An assembly of 90 such fuel elements forms a hexagonal core. The core is surrounded by a thermal radiation shield, a thin thermal neutron absorber, and a BeO reflector containing boron-loaded control drums.

Integrity of nuclear genomic deoxyribonucleic acid in cooked meat: Implications for food traceability.

PubMed

Aslan, O; Hamill, R M; Sweeney, T; Reardon, W; Mullen, A M

2009-01-01

It is essential to isolate high-quality DNA from muscle tissue for PCR-based applications in traceability of animal origin. We wished to examine the impact of cooking meat to a range of core temperatures on the quality and quantity of subsequently isolated genomic (specifically, nuclear) DNA. Triplicate steak samples were cooked in a water bath (100 degrees C) until their final internal temperature was 75, 80, 85, 90, 95, or 100 degrees C, and DNA was extracted. Deoxyribonucleic acid quantity was significantly reduced in cooked meat samples compared with raw (6.5 vs. 56.6 ng/microL; P < 0.001), but there was no relationship with cooking temperature. Quality (A(260)/A(280), i.e., absorbance at 260 and 280 nm) was also affected by cooking (P < 0.001). For all 3 genes, large PCR amplicons (product size >800 bp) were observed only when using DNA from raw meat and steak cooked to lower core temperatures. Small amplicons (<200 bp) were present for all core temperatures. Cooking meat to high temperatures thus resulted in a reduced overall yield and probable fragmentation of DNA to sizes less than 800 bp. Although nuclear DNA is preferable to mitochondrial DNA for food authentication, it is less abundant, and results suggest that analyses should be designed to use small amplicon sizes for meat cooked to high core temperatures.

Using the sound of nuclear energy

DOE PAGES

Garrett, Steven; Smith, James; Smith, Robert; ...

2016-08-01

The generation of sound by heat has been documented as an “acoustical curiosity” since a Buddhist monk reported the loud tone generated by a ceremonial rice-cooker in his diary, in 1568. Over the last four decades, significant progress has been made in understanding “thermoacoustic processes,” enabling the design of thermoacoustic engines and refrigerators. Motivated by the Fukushima nuclear reactor disaster, we have developed and tested a thermoacoustic engine that exploits the energy-rich conditions in the core of a nuclear reactor to provide core condition information to the operators without a need for external electrical power. The heat engine is self-poweredmore » and can wirelessly transmit the temperature and reactor power level by generation of a pure tone which can be detected outside the reactor. We report here the first use of a fission-powered thermoacoustic engine capable of serving as a performance and safety sensor in the core of a research reactor and present data from the hydrophones in the coolant (far from the core) and an accelerometer attached to a structure outside the reactor. These measurements confirmed that the frequency of the sound produced indicates the reactor’s coolant temperature and that the amplitude (above an onset threshold) is related to the reactor’s operating power level. Furthermore, these signals can be detected even in the presence of substantial background noise generated by the reactor’s fluid pumps.« less

Using the sound of nuclear energy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garrett, Steven; Smith, James; Smith, Robert

The generation of sound by heat has been documented as an “acoustical curiosity” since a Buddhist monk reported the loud tone generated by a ceremonial rice-cooker in his diary, in 1568. Over the last four decades, significant progress has been made in understanding “thermoacoustic processes,” enabling the design of thermoacoustic engines and refrigerators. Motivated by the Fukushima nuclear reactor disaster, we have developed and tested a thermoacoustic engine that exploits the energy-rich conditions in the core of a nuclear reactor to provide core condition information to the operators without a need for external electrical power. The heat engine is self-poweredmore » and can wirelessly transmit the temperature and reactor power level by generation of a pure tone which can be detected outside the reactor. We report here the first use of a fission-powered thermoacoustic engine capable of serving as a performance and safety sensor in the core of a research reactor and present data from the hydrophones in the coolant (far from the core) and an accelerometer attached to a structure outside the reactor. These measurements confirmed that the frequency of the sound produced indicates the reactor’s coolant temperature and that the amplitude (above an onset threshold) is related to the reactor’s operating power level. Furthermore, these signals can be detected even in the presence of substantial background noise generated by the reactor’s fluid pumps.« less

Sub-saturation matter in compact stars: Nuclear modelling in the framework of the extended Thomas-Fermi theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aymard, François; Gulminelli, Francesca; Margueron, Jérôme

A recently introduced analytical model for the nuclear density profile [1] is implemented in the Extended Thomas-Fermi (ETF) energy density functional. This allows to (i) shed a new light on the issue of the sign of surface symmetry energy in nuclear mass formulas, as well as to (ii) show the importance of the in-medium corrections to the nuclear cluster energies in thermodynamic conditions relevant for the description of core-collapse supernovae and (proto)-neutron star crust.

Nuclear astrophysics and electron beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwenk, A.

Electron beams provide important probes and constraints for nuclear astrophysics. This is especially exciting at energies within the regime of chiral effective field theory (EFT), which provides a systematic expansion for nuclear forces and electroweak operators based on quantum chromodynamics. This talk discusses some recent highlights and future directions based on chiral EFT, including nuclear structure and reactions for astrophysics, the neutron skin and constraints for the properties of neutron-rich matter in neutron stars and core-collapse supernovae, and the dark matter response of nuclei.

Sub-saturation matter in compact stars: Nuclear modelling in the framework of the extended Thomas-Fermi theory

NASA Astrophysics Data System (ADS)

Aymard, François; Gulminelli, Francesca; Margueron, Jérôme

2015-02-01

A recently introduced analytical model for the nuclear density profile [1] is implemented in the Extended Thomas-Fermi (ETF) energy density functional. This allows to (i) shed a new light on the issue of the sign of surface symmetry energy in nuclear mass formulas, as well as to (ii) show the importance of the in-medium corrections to the nuclear cluster energies in thermodynamic conditions relevant for the description of core-collapse supernovae and (proto)-neutron star crust.

Where do the Neutrinos go?

NASA Astrophysics Data System (ADS)

Freedman, Stuart

2011-10-01

Everybody knows that nuclear physics is the study the kind of matter found inside the atomic nucleus whether they it is at the center of atoms or the core of neutron stars. Nevertheless, nuclear physicists have made important discoveries about the neutrino. Figuring out where the neutrinos go in nuclear physics has challenged nuclear scientists, policy makers and those responsible for funding the enterprise. I will consider these and other challenges and how insightful scientific management has contributed the feast of wonderful discoveries about the neutrino.

The nuclear activity and central structure of the elliptical galaxy NGC 5322

NASA Astrophysics Data System (ADS)

Dullo, Bililign T.; Knapen, Johan H.; Williams, David R. A.; Beswick, Robert J.; Bendo, George; Baldi, Ranieri D.; Argo, Megan; McHardy, Ian M.; Muxlow, Tom; Westcott, J.

2018-04-01

We have analysed a new high-resolution e-MERLIN 1.5 GHz radio continuum map together with HST and SDSS imaging of NGC 5322, an elliptical galaxy hosting radio jets, aiming to understand the galaxy's central structure and its connection to the nuclear activity. We decomposed the composite HST + SDSS surface brightness profile of the galaxy into an inner stellar disc, a spheroid, and an outer stellar halo. Past works showed that this embedded disc counter-rotates rapidly with respect to the spheroid. The HST images reveal an edge-on nuclear dust disc across the centre, aligned along the major-axis of the galaxy and nearly perpendicular to the radio jets. After careful masking of this dust disc, we find a central stellar mass deficit Mdef in the spheroid, scoured by SMBH binaries with final mass MBH such that Mdef/MBH ˜ 1.3-3.4. We propose a three-phase formation scenario for NGC 5322, where a few (2-7) `dry' major mergers involving SMBHs built the spheroid with a depleted core. The cannibalism of a gas-rich satellite subsequently creates the faint counter-rotating disc and funnels gaseous material directly on to the AGN, powering the radio core with a brightness temperature of TB, core ˜ 4.5 × 107 K and the low-power radio jets (Pjets ˜ 7.04 × 1020 W Hz-1), which extend ˜1.6 kpc. The outer halo can later grow via minor mergers and the accretion of tidal debris. The low-luminosity AGN/jet-driven feedback may have quenched the late-time nuclear star formation promptly, which could otherwise have replenished the depleted core.

Geoantineutrino spectrum and slow nuclear burning on the boundary of the liquid and solid phases of the Earth's core

NASA Astrophysics Data System (ADS)

Rusov, V. D.; Pavlovich, V. N.; Vaschenko, V. N.; Tarasov, V. A.; Zelentsova, T. N.; Bolshakov, V. N.; Litvinov, D. A.; Kosenko, S. I.; Byegunova, O. A.

2007-09-01

We give an alternative description of the data produced in the KamLAND experiment. Assuming the existence of a natural nuclear reactor on the boundary of the liquid and solid phases of the Earth's core, a geoantineutrino spectrum is obtained. This assumption is based on the experimental results of V. Anisichkin and his collaborators on the interaction of uranium dioxide and uranium carbide with iron-nickel and silica-alumina melts at high pressure (5-10 GPa) and temperature (1600-2200°C), which led to the proposal of the existence of an actinide shell in the Earth's core. We describe the operating mechanism of this reactor as solitary waves of nuclear burning in 238U and/or 232Th medium, in particular, as neutron fission progressive waves of Feoktistov and/or Teller et al. type. Next, we propose a simplified model for the accumulation and burn-up kinetics in Feoktistov's U-Pu fuel cycle. We also apply this model for numerical simulations of neutron fission wave in a two-phase UO2/Fe medium on the surface of the Earth's solid core. The proposed georeactor model offers a mechanism for the generation of 3He. The 3He/4He distribution in the Earth's interior is calculated, which in turn can be used as a natural quantitative criterion of the georeactor thermal power. Finally, we give a tentative estimation of the geoantineutrino intensity and spectrum on the Earth's surface. For this purpose we use the O'Nions et al. geochemical model of mantle differentiation and crust growth complemented by a nuclear energy source (georeactor with power of 30 TW).

Spectral and kinetic effects accompanying the assembly of core complexes of Rhodobacter sphaeroides.

PubMed

Freiberg, Arvi; Chenchiliyan, Manoop; Rätsep, Margus; Timpmann, Kõu

2016-11-01

In the present work, spectral and kinetic changes accompanying the assembly of the light-harvesting 1 (LH1) complex with the reaction center (RC) complex into monomeric RC-LH1 and dimeric RC-LH1-PufX core complexes of the photosynthetic purple bacterium Rhodobacter sphaeroides are systematically studied over the temperature range of 4.5-300K. The samples were interrogated with a combination of optical absorption, hole burning, fluorescence excitation, steady state and picosecond time resolved fluorescence spectroscopy. Fair additivity of the LH1 and RC absorption spectra suggests rather weak electronic coupling between them. A low-energy tail revealed at cryogenic temperatures in the absorption spectra of both monomeric and dimeric core complexes is proved to be due to the special pair of the RC. At selected excitation intensity and temperature, the fluorescence decay time of core complexes is shown to be a function of multiple factors, most importantly of the presence/absence of RCs, the supramolecular architecture (monomeric or dimeric) of the complexes, and whether the complexes were studied in a native membrane environment or in a detergent - purified state. Copyright © 2016 Elsevier B.V. All rights reserved.

The neutrino opacity of neutron rich matter

NASA Astrophysics Data System (ADS)

Alcain, P. N.; Dorso, C. O.

2017-05-01

The study of neutron rich matter, present in neutron star, proto-neutron stars and core-collapse supernovae, can lead to further understanding of the behavior of nuclear matter in highly asymmetric nuclei. Heterogeneous structures are expected to exist in these systems, often referred to as nuclear pasta. We have carried out a systematic study of neutrino opacity for different thermodynamic conditions in order to assess the impact that the structure has on it. We studied the dynamics of the neutrino opacity of the heterogeneous matter at different thermodynamic conditions with semiclassical molecular dynamics model already used to study nuclear multifragmentation. For different densities, proton fractions and temperature, we calculate the very long range opacity and the cluster distribution. The neutrino opacity is of crucial importance for the evolution of the core-collapse supernovae and the neutrino scattering.

Has First-Grade Core Reading Program Text Complexity Changed across Six Decades?

ERIC Educational Resources Information Center

Fitzgerald, Jill; Elmore, Jeff; Relyea, Jackie Eunjung; Hiebert, Elfrieda H.; Stenner, A. Jackson

2016-01-01

The purpose of the study was to address possible text complexity shifts across the past six decades for a continually best-selling first-grade core reading program. The anthologies of one publisher's seven first-grade core reading programs were examined using computer-based analytics, dating from 1962 to 2013. Variables were Overall Text…

The novel implicit LU-SGS parallel iterative method based on the diffusion equation of a nuclear reactor on a GPU cluster

NASA Astrophysics Data System (ADS)

Zhang, Jilin; Sha, Chaoqun; Wu, Yusen; Wan, Jian; Zhou, Li; Ren, Yongjian; Si, Huayou; Yin, Yuyu; Jing, Ya

2017-02-01

GPU not only is used in the field of graphic technology but also has been widely used in areas needing a large number of numerical calculations. In the energy industry, because of low carbon, high energy density, high duration and other characteristics, the development of nuclear energy cannot easily be replaced by other energy sources. Management of core fuel is one of the major areas of concern in a nuclear power plant, and it is directly related to the economic benefits and cost of nuclear power. The large-scale reactor core expansion equation is large and complicated, so the calculation of the diffusion equation is crucial in the core fuel management process. In this paper, we use CUDA programming technology on a GPU cluster to run the LU-SGS parallel iterative calculation against the background of the diffusion equation of the reactor. We divide one-dimensional and two-dimensional mesh into a plurality of domains, with each domain evenly distributed on the GPU blocks. A parallel collision scheme is put forward that defines the virtual boundary of the grid exchange information and data transmission by non-stop collision. Compared with the serial program, the experiment shows that GPU greatly improves the efficiency of program execution and verifies that GPU is playing a much more important role in the field of numerical calculations.

Coated ceramic breeder materials

DOEpatents

Tam, Shiu-Wing; Johnson, Carl E.

1987-01-01

A breeder material for use in a breeder blanket of a nuclear reactor is disclosed. The breeder material comprises a core material of lithium containing ceramic particles which has been coated with a neutron multiplier such as Be or BeO, which coating has a higher thermal conductivity than the core material.

Coated ceramic breeder materials

DOEpatents

Tam, Shiu-Wing; Johnson, Carl E.

1987-04-07

A breeder material for use in a breeder blanket of a nuclear reactor is disclosed. The breeder material comprises a core material of lithium containing ceramic particles which has been coated with a neutron multiplier such as Be or BeO, which coating has a higher thermal conductivity than the core material.

Super-resolution microscopy reveals LINC complex recruitment at nuclear indentation sites.

PubMed

Versaevel, Marie; Braquenier, Jean-Baptiste; Riaz, Maryam; Grevesse, Thomas; Lantoine, Joséphine; Gabriele, Sylvain

2014-12-08

Increasing evidences show that the actin cytoskeleton is a key parameter of the nuclear remodeling process in response to the modifications of cellular morphology. However, detailed information on the interaction between the actin cytoskeleton and the nuclear lamina was still lacking. We addressed this question by constraining endothelial cells on rectangular fibronectin-coated micropatterns and then using Structured Illumination Microscopy (SIM) to observe the interactions between actin stress fibers, nuclear lamina and LINC complexes at a super-resolution scale. Our results show that tension in apical actin stress fibers leads to deep nuclear indentations that significantly deform the nuclear lamina. Interestingly, indented nuclear zones are characterized by a local enrichment of LINC complexes, which anchor apical actin fibers to the nuclear lamina. Moreover, our findings indicate that nuclear indentations induce the formation of segregated domains of condensed chromatin. However, nuclear indentations and condensed chromatin domains are not irreversible processes and both can relax in absence of tension in apical actin stress fibers.

Nipah and Hendra Virus Nucleoproteins Inhibit Nuclear Accumulation of Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 by Interfering with Their Complex Formation

PubMed Central

Sugai, Akihiro; Sato, Hiroki; Takayama, Ikuyo; Yoneda, Misako

2017-01-01

ABSTRACT Henipaviruses, such as Nipah (NiV) and Hendra (HeV) viruses, are highly pathogenic zoonotic agents within the Paramyxoviridae family. The phosphoprotein (P) gene products of the paramyxoviruses have been well characterized for their interferon (IFN) antagonist activity and their contribution to viral pathogenicity. In this study, we demonstrated that the nucleoprotein (N) of henipaviruses also prevents the host IFN signaling response. Reporter assays demonstrated that the NiV and HeV N proteins (NiV-N and HeV-N, respectively) dose-dependently suppressed both type I and type II IFN responses and that the inhibitory effect was mediated by their core domains. Additionally, NiV-N prevented the nuclear transport of signal transducer and activator of transcription 1 (STAT1) and STAT2. However, NiV-N did not associate with Impα5, Impβ1, or Ran, which are members of the nuclear transport system for STATs. Although P protein is known as a binding partner of N protein and actively retains N protein in the cytoplasm, the IFN antagonist activity of N protein was not abolished by the coexpression of P protein. This suggests that the IFN inhibition by N protein occurs in the cytoplasm. Furthermore, we demonstrated that the complex formation of STATs was hampered in the N protein-expressing cells. As a result, STAT nuclear accumulation was reduced, causing a subsequent downregulation of interferon-stimulated genes (ISGs) due to low promoter occupancy by STAT complexes. This novel route for preventing host IFN responses by henipavirus N proteins provides new insight into the pathogenesis of these viruses. IMPORTANCE Paramyxoviruses are well known for suppressing interferon (IFN)-mediated innate immunity with their phosphoprotein (P) gene products, and the henipaviruses also possess P, V, W, and C proteins for evading host antiviral responses. There are numerous studies providing evidence for the relationship between viral pathogenicity and antagonistic activities against IFN responses by P gene products. Meanwhile, little attention has been paid to the influence of nucleoprotein (N) on host innate immune responses. In this study, we demonstrated that both the NiV and HeV N proteins have antagonistic activity against the JAK/STAT signaling pathway by preventing the nucleocytoplasmic trafficking of STAT1 and STAT2. This inhibitory effect is due to an impairment of the ability of STATs to form complexes. These results provide new insight into the involvement of N protein in viral pathogenicity via its IFN antagonism. PMID:28835499

Nipah and Hendra Virus Nucleoproteins Inhibit Nuclear Accumulation of Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 by Interfering with Their Complex Formation.

PubMed

Sugai, Akihiro; Sato, Hiroki; Takayama, Ikuyo; Yoneda, Misako; Kai, Chieko

2017-11-01

Henipaviruses, such as Nipah (NiV) and Hendra (HeV) viruses, are highly pathogenic zoonotic agents within the Paramyxoviridae family. The phosphoprotein (P) gene products of the paramyxoviruses have been well characterized for their interferon (IFN) antagonist activity and their contribution to viral pathogenicity. In this study, we demonstrated that the nucleoprotein (N) of henipaviruses also prevents the host IFN signaling response. Reporter assays demonstrated that the NiV and HeV N proteins (NiV-N and HeV-N, respectively) dose-dependently suppressed both type I and type II IFN responses and that the inhibitory effect was mediated by their core domains. Additionally, NiV-N prevented the nuclear transport of signal transducer and activator of transcription 1 (STAT1) and STAT2. However, NiV-N did not associate with Impα5, Impβ1, or Ran, which are members of the nuclear transport system for STATs. Although P protein is known as a binding partner of N protein and actively retains N protein in the cytoplasm, the IFN antagonist activity of N protein was not abolished by the coexpression of P protein. This suggests that the IFN inhibition by N protein occurs in the cytoplasm. Furthermore, we demonstrated that the complex formation of STATs was hampered in the N protein-expressing cells. As a result, STAT nuclear accumulation was reduced, causing a subsequent downregulation of interferon-stimulated genes (ISGs) due to low promoter occupancy by STAT complexes. This novel route for preventing host IFN responses by henipavirus N proteins provides new insight into the pathogenesis of these viruses. IMPORTANCE Paramyxoviruses are well known for suppressing interferon (IFN)-mediated innate immunity with their phosphoprotein (P) gene products, and the henipaviruses also possess P, V, W, and C proteins for evading host antiviral responses. There are numerous studies providing evidence for the relationship between viral pathogenicity and antagonistic activities against IFN responses by P gene products. Meanwhile, little attention has been paid to the influence of nucleoprotein (N) on host innate immune responses. In this study, we demonstrated that both the NiV and HeV N proteins have antagonistic activity against the JAK/STAT signaling pathway by preventing the nucleocytoplasmic trafficking of STAT1 and STAT2. This inhibitory effect is due to an impairment of the ability of STATs to form complexes. These results provide new insight into the involvement of N protein in viral pathogenicity via its IFN antagonism. Copyright © 2017 American Society for Microbiology.

A Proteasome Cap Subunit Required for Spindle Pole Body Duplication in Yeast

PubMed Central

McDonald, Heather B.; Byers, Breck

1997-01-01

Proteasome-mediated protein degradation is a key regulatory mechanism in a diversity of complex processes, including the control of cell cycle progression. The selection of substrates for degradation clearly depends on the specificity of ubiquitination mechanisms, but further regulation may occur within the proteasomal 19S cap complexes, which attach to the ends of the 20S proteolytic core and are thought to control entry of substrates into the core. We have characterized a gene from Saccharomyces cerevisiae that displays extensive sequence similarity to members of a family of ATPases that are components of the 19S complex, including human subunit p42 and S. cerevisiae SUG1/ CIM3 and CIM5 products. This gene, termed PCS1 (for proteasomal cap subunit), is identical to the recently described SUG2 gene (Russell, S.J., U.G. Sathyanarayana, and S.A. Johnston. 1996. J. Biol. Chem. 271:32810– 32817). We have shown that PCS1 function is essential for viability. A temperature-sensitive pcs1 strain arrests principally in the second cycle after transfer to the restrictive temperature, blocking as large-budded cells with a G2 content of unsegregated DNA. EM reveals that each arrested pcs1 cell has failed to duplicate its spindle pole body (SPB), which becomes enlarged as in other monopolar mutants. Additionally, we have shown localization of a functional Pcs1–green fluorescent protein fusion to the nucleus throughout the cell cycle. We hypothesize that Pcs1p plays a role in the degradation of certain potentially nuclear component(s) in a manner that specifically is required for SPB duplication. PMID:9151663

Genetics Home Reference: mitochondrial complex III deficiency

MedlinePlus

... DNA packaged in chromosomes within the cell nucleus (nuclear DNA). It is not clear why the severity ... deficiency Genetic Testing Registry: Mitochondrial complex III deficiency, nuclear type 2 Genetic Testing Registry: Mitochondrial complex III ...

Monomeric RC-LH1 core complexes retard LH2 assembly and intracytoplasmic membrane formation in PufX-minus mutants of Rhodobacter sphaeroides

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, Peter G.; Mothersole, David J.; Ng, Irene W.

2011-01-01

In the model photosynthetic bacterium Rhodobacter sphaeroides domains of light-harvesting 2 (LH2) complexes surround and interconnect dimeric reaction centre–light-harvesting 1–PufX (RC–LH1–PufX) ‘core’ complexes, forming extensive networks for energy transfer and trapping. These complexes are housed in spherical intracytoplasmic membranes (ICMs), which are assembled in a stepwise process where biosynthesis of core complexes tends to dominate the early stages of membrane invagination. The kinetics of LH2 assembly were measured in PufX mutants that assemble monomeric core complexes, as a consequence of either a twelve-residue N-terminal truncation of PufX (PufXΔ12) or the complete removal of PufX (PufX -). Lower rates of LH2more » assembly and retarded maturation of membrane invagination were observed for the larger and less curved ICM from the PufX - mutant, consistent with the proposition that local membrane curvature, initiated by arrays of bent RC–LH1–PufX dimers, creates a favourable environment for stable assembly of LH2 complexes. Transmission electron microscopy and high-resolution atomic force microscopy were used to examine ICM morphology and membrane protein organisation in these mutants. Some partitioning of core and LH2 complexes was observed in PufX - membranes, resulting in locally ordered clusters of monomeric RC–LH1 complexes. The distribution of core and LH2 complexes in the three types of membrane examined is consistent with previous models of membrane curvature and domain formation (Frese et al., 2008), which demonstrated that a combination of crowding and asymmetries in sizes and shapes of membrane protein complexes drives membrane organisation.« less

Interactions of Escherichia coli σ70 within the transcription elongation complex

PubMed Central

Daube, Shirley S.; von Hippel, Peter H.

1999-01-01

A functional transcription elongation complex can be formed without passing through a promoter by adding a complementary RNA primer and core Escherichia coli RNA polymerase in trans to an RNA-primed synthetic bubble-duplex DNA framework. This framework consists of a double-stranded DNA sequence with an internal noncomplementary DNA “bubble” containing a hybridized RNA primer. On addition of core polymerase and the requisite NTPs, the RNA primer is extended in a process that manifests most of the properties of in vitro transcription elongation. This synthetic elongation complex can also be assembled by using holo rather than core RNA polymerase, and in this study we examine the interactions and fate of the σ70 specificity subunit of the holopolymerase in the assembly process. We show that the addition of holopolymerase to the bubble-duplex construct triggers the dissociation of the sigma factor from some complexes, whereas in others the RNA oligomer is released into solution instead. These results are consistent with an allosteric competition between σ70 and the nascent RNA strand within the elongation complex and suggest that both cannot be bound to the core polymerase simultaneously. However, the dissociation of σ70 from the complex can also be stimulated by binding of the holopolymerase to the DNA bubble duplex in the absence of a hybridized RNA primer, suggesting that the binding of the core polymerase to the bubble-duplex construct also triggers a conformational change that additionally weakens the sigma–core interaction. PMID:10411885

Propellant actuated nuclear reactor steam depressurization valve

DOEpatents

Ehrke, Alan C.; Knepp, John B.; Skoda, George I.

1992-01-01

A nuclear fission reactor combined with a propellant actuated depressurization and/or water injection valve is disclosed. The depressurization valve releases pressure from a water cooled, steam producing nuclear reactor when required to insure the safety of the reactor. Depressurization of the reactor pressure vessel enables gravity feeding of supplementary coolant water through the water injection valve to the reactor pressure vessel to prevent damage to the fuel core.

A Review of Gas-Cooled Reactor Concepts for SDI Applications

DTIC Science & Technology

1989-08-01

710 program .) Wire- Core Reactor (proposed by Rockwell). The wire- core reactor utilizes thin fuel wires woven between spacer wires to form an open...reactor is based on results of developmental studies of nuclear rocket propulsion systems. The reactor core is made up of annular fuel assemblies of...XE Addendum to Volume II. NERVA Fuel Development , Westinghouse Astronuclear Laboratory, TNR-230, July 15’ 1972. J I8- Rover Program Reactor Tests

Main steam-line break core shroud loading calculations for BWRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shoop, U.; Feltus, M.A.; Baratta, A.J.

1995-12-31

In July 1994, the U.S. Nuclear regulatory Commission sent out Generic Letter 94-03 to all boiling water reactors in the United States, informing them of intergranular stress corrosion cracking of core shrouds found in 2 reactors. The letter directed all to perform safety analysis of the BWR units. Penn State performed scoping calculations to determine the forces experienced by the core shroud during a main-stream line break transient.

Analytical measurements of fission products during a severe nuclear accident

NASA Astrophysics Data System (ADS)

Doizi, D.; Reymond la Ruinaz, S.; Haykal, I.; Manceron, L.; Perrin, A.; Boudon, V.; Vander Auwera, J.; tchana, F. Kwabia; Faye, M.

2018-01-01

The Fukushima accident emphasized the fact that ways to monitor in real time the evolution of a nuclear reactor during a severe accident remain to be developed. No fission products were monitored during twelve days; only dose rates were measured, which is not sufficient to carry out an online diagnosis of the event. The first measurements were announced with little reliability for low volatile fission products. In order to improve the safety of nuclear plants and minimize the industrial, ecological and health consequences of a severe accident, it is necessary to develop new reliable measurement systems, operating at the earliest and closest to the emission source of fission products. Through the French program ANR « Projet d'Investissement d'Avenir », the aim of the DECA-PF project (diagnosis of core degradation from fission products measurements) is to monitor in real time the release of the major fission products (krypton, xenon, gaseous forms of iodine and ruthenium) outside the nuclear reactor containment. These products are released at different times during a nuclear accident and at different states of the nuclear core degradation. Thus, monitoring these fission products gives information on the situation inside the containment and helps to apply the Severe Accident Management procedures. Analytical techniques have been proposed and evaluated. The results are discussed here.

Multi-layered nanoparticles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process.

PubMed

Akita, Hidetaka; Kudo, Asako; Minoura, Arisa; Yamaguti, Masaya; Khalil, Ikramy A; Moriguchi, Rumiko; Masuda, Tomoya; Danev, Radostin; Nagayama, Kuniaki; Kogure, Kentaro; Harashima, Hideyoshi

2009-05-01

Efficient targeting of DNA to the nucleus is a prerequisite for effective gene therapy. The gene-delivery vehicle must penetrate through the plasma membrane, and the DNA-impermeable double-membraned nuclear envelope, and deposit its DNA cargo in a form ready for transcription. Here we introduce a concept for overcoming intracellular membrane barriers that involves step-wise membrane fusion. To achieve this, a nanotechnology was developed that creates a multi-layered nanoparticle, which we refer to as a Tetra-lamellar Multi-functional Envelope-type Nano Device (T-MEND). The critical structural elements of the T-MEND are a DNA-polycation condensed core coated with two nuclear membrane-fusogenic inner envelopes and two endosome-fusogenic outer envelopes, which are shed in stepwise fashion. A double-lamellar membrane structure is required for nuclear delivery via the stepwise fusion of double layered nuclear membrane structure. Intracellular membrane fusions to endosomes and nuclear membranes were verified by spectral imaging of fluorescence resonance energy transfer (FRET) between donor and acceptor fluorophores that had been dually labeled on the liposome surface. Coating the core with the minimum number of nucleus-fusogenic lipid envelopes (i.e., 2) is essential to facilitate transcription. As a result, the T-MEND achieves dramatic levels of transgene expression in non-dividing cells.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorr, Kent A.; Ostrom, Michael J.; Freeman-Pollard, Jhivaun R.

CH2M Hill Plateau Remediation Company (CHPRC) designed, constructed, commissioned, and began operation of the largest groundwater pump and treatment facility in the U.S. Department of Energy’s (DOE) nationwide complex. This one-of-a-kind groundwater pump and treatment facility, located at the Hanford Nuclear Reservation Site (Hanford Site) in Washington State, was built to an accelerated schedule with American Recovery and Reinvestment Act (ARRA) funds. There were many contractual, technical, configuration management, quality, safety, and Leadership in Energy and Environmental Design (LEED) challenges associated with the design, procurement, construction, and commissioning of this $95 million, 52,000 ft groundwater pump and treatment facility tomore » meet DOE’s mission objective of treating contaminated groundwater at the Hanford Site with a new facility by June 28, 2012. The project team’s successful integration of the project’s core values and green energy technology throughout design, procurement, construction, and start-up of this complex, first-of-its-kind Bio Process facility resulted in successful achievement of DOE’s mission objective, as well as attainment of LEED GOLD certification, which makes this Bio Process facility the first non-administrative building in the DOE Office of Environmental Management complex to earn such an award.« less

Tissue-Specific Regulation of Chromatin Insulator Function

PubMed Central

Matzat, Leah H.; Dale, Ryan K.; Moshkovich, Nellie; Lei, Elissa P.

2012-01-01

Chromatin insulators organize the genome into distinct transcriptional domains and contribute to cell type–specific chromatin organization. However, factors regulating tissue-specific insulator function have not yet been discovered. Here we identify the RNA recognition motif-containing protein Shep as a direct interactor of two individual components of the gypsy insulator complex in Drosophila. Mutation of shep improves gypsy-dependent enhancer blocking, indicating a role as a negative regulator of insulator activity. Unlike ubiquitously expressed core gypsy insulator proteins, Shep is highly expressed in the central nervous system (CNS) with lower expression in other tissues. We developed a novel, quantitative tissue-specific barrier assay to demonstrate that Shep functions as a negative regulator of insulator activity in the CNS but not in muscle tissue. Additionally, mutation of shep alters insulator complex nuclear localization in the CNS but has no effect in other tissues. Consistent with negative regulatory activity, ChIP–seq analysis of Shep in a CNS-derived cell line indicates substantial genome-wide colocalization with a single gypsy insulator component but limited overlap with intact insulator complexes. Taken together, these data reveal a novel, tissue-specific mode of regulation of a chromatin insulator. PMID:23209434

Determination of parameters of a nuclear reactor through noise measurements

DOEpatents

Cohn, C.E.

1975-07-15

A method of measuring parameters of a nuclear reactor by noise measurements is described. Noise signals are developed by the detectors placed in the reactor core. The polarity coincidence between the noise signals is used to develop quantities from which various parameters of the reactor can be calculated. (auth)

Many-particle theory of nuclear system with application to neutron-star matter and other systems

NASA Technical Reports Server (NTRS)

Yang, C. H.

1978-01-01

General problems in nuclear-many-body theory were considered. Superfluid states of neutron star matter and other strongly interacting many-fermion systems were analyzed by using the soft-core potential of Reid. The pion condensation in neutron star matter was also treated.

77 FR 41670 - Definition of Terms

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

... cryptography'', 2. On page 642, add the term ``Explosives'', 3. On page 650, add the term ``Nuclear reactor... ``Commerce Control List''. * * * * * Nuclear reactor. (Cat 0 and 2) includes the items within or attached directly to the reactor vessel, the equipment which controls the level of power in the core, and the...

Heat dissipating nuclear reactor with metal liner

DOEpatents

Gluekler, E.L.; Hunsbedt, A.; Lazarus, J.D.

1985-11-21

A nuclear reactor containment including a reactor vessel disposed within a cavity with capability for complete inherent decay heat removal in the earth and surrounded by a cast steel containment member which surrounds the vessel is described in this disclosure. The member has a thick basemat in contact with metal pilings. The basemat rests on a bed of porous particulate material, into which water is fed to produce steam which is vented to the atmosphere. There is a gap between the reactor vessel and the steel containment member. The containment member holds any sodium or core debris escaping from the reactor vessel if the core melts and breaches the vessel.

Realistic Testing of the Safe Affordable Fission Engine (SAFE-100) Thermal Simulator Using Fiber Bragg Gratings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stinson-Bagby, Kelly L.; Fielder, Robert S.; Van Dyke, Melissa K.

2004-02-04

The motivation for the reported research was to support NASA space nuclear power initiatives through the development of advanced fiber optic sensors for space-based nuclear power applications. Distributed high temperature measurements were made with 20 FBG temperature sensors installed in the SAFE-100 thermal simulator at the NASA Marshal Space Flight Center. Experiments were performed at temperatures approaching 800 deg. C and 1150 deg. C for characterization studies of the SAFE-100 core. Temperature profiles were successfully generated for the core during temperature increases and decreases. Related tests in the SAFE-100 successfully provided strain measurement data.

Effect of buoyancy on fuel containment in an open-cycle gas-core nuclear rocket engine.

NASA Technical Reports Server (NTRS)

Putre, H. A.

1971-01-01

Analysis aimed at determining the scaling laws for the buoyancy effect on fuel containment in an open-cycle gas-core nuclear rocket engine, so conducted that experimental conditions can be related to engine conditions. The fuel volume fraction in a short coaxial flow cavity is calculated with a programmed numerical solution of the steady Navier-Stokes equations for isothermal, variable density fluid mixing. A dimensionless parameter B, called the Buoyancy number, was found to correlate the fuel volume fraction for large accelerations and various density ratios. This parameter has the value B = 0 for zero acceleration, and B = 350 for typical engine conditions.

Heat dissipating nuclear reactor with metal liner

DOEpatents

Gluekler, Emil L.; Hunsbedt, Anstein; Lazarus, Jonathan D.

1987-01-01

Disclosed is a nuclear reactor containment including a reactor vessel disposed within a cavity with capability for complete inherent decay heat removal in the earth and surrounded by a cast steel containment member which surrounds the vessel. The member has a thick basemat in contact with metal pilings. The basemat rests on a bed of porous particulate material, into which water is fed to produce steam which is vented to the atmosphere. There is a gap between the reactor vessel and the steel containment member. The containment member holds any sodium or core debris escaping from the reactor vessel if the core melts and breaches the vessel.

FUEL ELEMENTS FOR NUCLEAR REACTORS

DOEpatents

Blainey, A.; Lloyd, H.

1961-07-11

A method of sheathing a tubular fuel element for a nuclear reactor is described. A low melting metal core member is centered in a die, a layer of a powdered sheathing substance is placed on the bottom of the die, the tubular fuel element is inserted in the die, the space between the tubular fuel element and the die walls and core member is filled with the same powdered sheathing substance, a layer of the same substance is placed over the fissile material, and the charge within the die is subjected to pressure in the direction of the axis of the fuel element at the sintering temperature of the protective substance.

Crystal structure of tetra-kis-(μ3-2-{[1,1-bis-(hy-droxy-meth-yl)-2-oxidoeth-yl]imino-meth-yl}phenolato)tetra-copper(II) ethanol monosolvate 2.5-hydrate.

PubMed

Wang, Weilun; Ran, Jingwen

2015-05-01

The title compound, [Cu4(C11H13NO4)4]·CH3CH2OH·2.5H2O, is an electronically neutral tetra-nuclear copper(II) complex with a cubane-like Cu4O4 core. The complete molecule has point group symmetry 2. The phenol hy-droxy group and one of the three alcohol hy-droxy groups of each 2-{[tris-(hy-droxy-meth-yl)meth-yl]imino-meth-yl}phenol ligand are depro-ton-ated, while the secondary amine and the other two hy-droxy groups remain unchanged. The Cu(II) atoms in the Cu4O4 core are connected by four μ3-O atoms from the deprotonated alcohol hy-droxy groups. Each of the penta-coordinated Cu(II) ions has an NO4 distorted square-pyramidal environment through coordination to the tridentate Schiff base ligands. The Cu-N/O bond lengths span the range 1.902 (4)-1.955 (4) Å, similar to values reported for related structures. There are O-H⋯O hydrogen-bond inter-actions between the complex molecules and the ethanol and water solvent molecules, leading to the formation of a three-dimensional network. The ethanol solvent molecule is disordered about a twofold rotation axis. One of the two independent water molecules is also located on this twofold rotation axis and shows half-occupancy.

Possible generation of heat from nuclear fusion in Earth’s inner core

PubMed Central

Fukuhara, Mikio

2016-01-01

The cause and source of the heat released from Earth’s interior have not yet been determined. Some research groups have proposed that the heat is supplied by radioactive decay or by a nuclear georeactor. Here we postulate that the generation of heat is the result of three-body nuclear fusion of deuterons confined in hexagonal FeDx core-centre crystals; the reaction rate is enhanced by the combined attraction effects of high-pressure (~364 GPa) and high-temperature (~5700 K) and by the physical catalysis of neutral pions: 2D + 2D + 2D → 21H + 4He + 2  + 20.85 MeV. The possible heat generation rate can be calculated as 8.12 × 1012 J/m3, based on the assumption that Earth’s primitive heat supply has already been exhausted. The H and He atoms produced and the anti-neutrino are incorporated as Fe-H based alloys in the H-rich portion of inner core, are released from Earth’s interior to the universe, and pass through Earth, respectively. PMID:27876860

Development and preliminary verification of the 3D core neutronic code: COCO

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, H.; Mo, K.; Li, W.

As the recent blooming economic growth and following environmental concerns (China)) is proactively pushing forward nuclear power development and encouraging the tapping of clean energy. Under this situation, CGNPC, as one of the largest energy enterprises in China, is planning to develop its own nuclear related technology in order to support more and more nuclear plants either under construction or being operation. This paper introduces the recent progress in software development for CGNPC. The focus is placed on the physical models and preliminary verification results during the recent development of the 3D Core Neutronic Code: COCO. In the COCO code,more » the non-linear Green's function method is employed to calculate the neutron flux. In order to use the discontinuity factor, the Neumann (second kind) boundary condition is utilized in the Green's function nodal method. Additionally, the COCO code also includes the necessary physical models, e.g. single-channel thermal-hydraulic module, burnup module, pin power reconstruction module and cross-section interpolation module. The preliminary verification result shows that the COCO code is sufficient for reactor core design and analysis for pressurized water reactor (PWR). (authors)« less

Student Reading Growth Illuminates the Common Core Text-Complexity Standard: Raising Both Bars

ERIC Educational Resources Information Center

Williamson, Gary L.; Fitzgerald, Jill; Stenner, Jackson A.

2014-01-01

The Common Core State Standards (CCSS) establish a challenging text-complexity standard for all high school graduates to read at college and workplace text-complexity levels. We argue that implementation of the CCSS standard requires concurrent examination of historical student reading-growth trends. An example of a historical student average…

Structural and functional organization of the ESCRT-I trafficking complex

PubMed Central

Kostelansky, Michael S.; Sun, Ji; Lee, Sangho; Kim, Jaewon; Ghirlando, Rodolfo; Hierro, Aitor; Emr, Scott D.; Hurley, James H.

2006-01-01

Summary The Endosomal Sorting Complex Required for Transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 Å resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEVdomain, the Vps28 C-domain, and other domains project to bind their partners. PMID:16615894

Crystal structure of the Xpo1p nuclear export complex bound to the SxFG/PxFG repeats of the nucleoporin Nup42p.

PubMed

Koyama, Masako; Hirano, Hidemi; Shirai, Natsuki; Matsuura, Yoshiyuki

2017-10-01

Xpo1p (yeast CRM1) is the major nuclear export receptor that carries a plethora of proteins and ribonucleoproteins from the nucleus to cytoplasm. The passage of the Xpo1p nuclear export complex through nuclear pore complexes (NPCs) is facilitated by interactions with nucleoporins (Nups) containing extensive repeats of phenylalanine-glycine (so-called FG repeats), although the precise role of each Nup in the nuclear export reaction remains incompletely understood. Here we report structural and biochemical characterization of the interactions between the Xpo1p nuclear export complex and the FG repeats of Nup42p, a nucleoporin localized at the cytoplasmic face of yeast NPCs and has characteristic SxFG/PxFG sequence repeat motif. The crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex identified three binding sites for the SxFG/PxFG repeats on HEAT repeats 14-20 of Xpo1p. Mutational analyses of Nup42p showed that the conserved serines and prolines in the SxFG/PxFG repeats contribute to Xpo1p-Nup42p binding. Our structural and biochemical data suggest that SxFG/PxFG-Nups such as Nup42p and Nup159p at the cytoplasmic face of NPCs provide high-affinity docking sites for the Xpo1p nuclear export complex in the terminal stage of NPC passage and that subsequent disassembly of the nuclear export complex facilitates recycling of free Xpo1p back to the nucleus. © 2017 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

A core hSSB1–INTS complex participates in the DNA damage response

PubMed Central

Zhang, Feng; Ma, Teng; Yu, Xiaochun

2013-01-01

Summary Human single-stranded DNA-binding protein 1 (hSSB1) plays an important role in the DNA damage response and the maintenance of genomic stability. It has been shown that the core hSSB1 complex contains hSSB1, INTS3 and C9orf80. Using protein affinity purification, we have identified integrator complex subunit 6 (INTS6) as a major subunit of the core hSSB1 complex. INTS6 forms a stable complex with INTS3 and hSSB1 both in vitro and in vivo. In this complex, INTS6 directly interacts with INTS3. In response to the DNA damage response, along with INTS3 and hSSB1, INTS6 relocates to the DNA damage sites. Moreover, the hSSB1–INTS complex regulates the accumulation of RAD51 and BRCA1 at DNA damage sites and the correlated homologous recombination. PMID:23986477

Inflammation and regeneration in the dentin-pulp complex: a double-edged sword.

PubMed

Cooper, Paul R; Holder, Michelle J; Smith, Anthony J

2014-04-01

Dental tissue infection and disease result in acute and chronic activation of the innate immune response, which is mediated by molecular and cellular signaling. Different cell types within the dentin-pulp complex are able to detect invading bacteria at all stages of the infection. Indeed, at relatively early disease stages, odontoblasts will respond to bacterial components, and as the disease progresses, core pulpal cells including fibroblasts, stems cells, endothelial cells, and immune cells will become involved. Pattern recognition receptors, such as Toll-like receptors expressed on these cell types, are responsible for detecting bacterial components, and their ligand binding leads to the activation of the nuclear factor-kappa B and p38 mitogen-activated protein (MAP) kinase intracellular signaling cascades. Subsequent nuclear translocation of the transcription factor subunits from these pathways will lead to proinflammatory mediator expression, including increases in cytokines and chemokines, which trigger host cellular defense mechanisms. The complex molecular signaling will result in the recruitment of immune system cells targeted at combating the invading microbes; however, the trafficking and antibacterial activity of these cells can lead to collateral tissue damage. Recent evidence suggests that if inflammation is resolved relatively low levels of proinflammatory mediators may promote tissue repair, whereas if chronic inflammation ensues repair mechanisms become inhibited. Thus, the effects of mediators are temporal context dependent. Although containment and removal of the infection are keys to enable dental tissue repair, it is feasible that the development of anti-inflammatory and immunomodulatory approaches, based on molecular, epigenetic, and photobiomodulatory technologies, may also be beneficial for future endodontic treatments. Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.

Support arrangement for core modules of nuclear reactors

DOEpatents

Bollinger, Lawrence R.

1987-01-01

A support arrangement is provided for the core modules of a nuclear reactor which provides support access through the control drive mechanisms of the reactor. This arrangement provides axial support of individual reactor core modules from the pressure vessel head in a manner which permits attachment and detachment of the modules from the head to be accomplished through the control drive mechanisms after their leadscrews have been removed. The arrangement includes a module support nut which is suspended from the pressure vessel head and screw threaded to the shroud housing for the module. A spline lock prevents loosening of the screw connection. An installation tool assembly, including a cell lifting and preloading tool and a torquing tool, fits through the control drive mechanism and provides lifting of the shroud housing while disconnecting the spline lock, as well as application of torque to the module support nut.

Support arrangements for core modules of nuclear reactors. [PWR

DOEpatents

Bollinger, L.R.

1983-11-03

A support arrangement is provided for the core modules of a nuclear reactor which provides support access through the control drive mechanisms of the reactor. This arrangement provides axial support of individual reactor core modules from the pressure vessel head in a manner which permits attachment and detachment of the modules from the head to be accomplished through the control drive mechanisms after their leadscrews have been removed. The arrangement includes a module support nut which is suspended from the pressure vessel head and screw threaded to the shroud housing for the module. A spline lock prevents loosening of the screw connection. An installation tool assembly, including a cell lifting and preloading tool and a torquing tool, fits through the control drive mechanism and provides lifting of the shroud housing while disconnecting the spline lock, as well as application of torque to the module support nut.

Effects of the Application of the New Nuclear Data Library ENDF/B to the Criticality Analysis of AP1000

NASA Astrophysics Data System (ADS)

Kuntoro, Iman; Sembiring, T. M.; Susilo, Jati; Deswandri; Sunaryo, G. R.

2018-02-01

Calculations of criticality of the AP1000 core due to the use of new edition of nuclear data library namely ENDF/B-VII and ENDF/B-VII.1 have been done. This work is aimed to know the accuracy of ENDF/B-VII.1 compared to ENDF/B-VII and ENDF/B-VI.8. in determining the criticality parameter of AP1000. Analysis ws imposed to core at cold zero power (CZP) conditions. The calculations have been carried out by means of MCNP computer code for 3 dimension geometry. The results show that criticality parameter namely effective multiplication factor of the AP1000 core are higher than that ones resulted from ENDF/B-VI.8 with relative differences of 0.39% for application of ENDF/B-VII and of 0.34% for application of ENDF/B-VII.1.

Post-Cold War Science and Technology at Los Alamos

NASA Astrophysics Data System (ADS)

Browne, John C.

2002-04-01

Los Alamos National Laboratory serves the nation through the development and application of leading-edge science and technology in support of national security. Our mission supports national security by: ensuring the safety, security, and reliability of the U.S. nuclear stockpile; reducing the threat of weapons of mass destruction in support of counter terrorism and homeland defense; and solving national energy, environment, infrastructure, and health security problems. We require crosscutting fundamental and advanced science and technology research to accomplish our mission. The Stockpile Stewardship Program develops and applies, advanced experimental science, computational simulation, and technology to ensure the safety and reliability of U.S. nuclear weapons in the absence of nuclear testing. This effort in itself is a grand challenge. However, the terrorist attack of September 11, 2001, reminded us of the importance of robust and vibrant research and development capabilities to meet new and evolving threats to our national security. Today through rapid prototyping we are applying new, innovative, science and technology for homeland defense, to address the threats of nuclear, chemical, and biological weapons globally. Synergistically, with the capabilities that we require for our core mission, we contribute in many other areas of scientific endeavor. For example, our Laboratory has been part of the NASA effort on mapping water on the moon and NSF/DOE projects studying high-energy astrophysical phenomena, understanding fundamental scaling phenomena of life, exploring high-temperature superconductors, investigating quantum information systems, applying neutrons to condensed-matter and nuclear physics research, developing large-scale modeling and simulations to understand complex phenomena, and exploring nanoscience that bridges the atomic to macroscopic scales. In this presentation, I will highlight some of these post-cold war science and technology advances including our national security contributions, and discuss some of challenges for Los Alamos in the future.

Influence of the chelator structures on the stability of Re and Tc tricarbonyl complexes with iminodiacetic acid tridentate ligands: a computational study.

PubMed

Hernández-Valdés, Daniel; Rodríguez-Riera, Zalua; Díaz-García, Alicia; Benoist, Eric; Jáuregui-Haza, Ulises

2016-08-01

The development of novel radiopharmaceuticals for nuclear medicine based on M(CO)3 (M = Tc, Re) complexes has attracted great attention. The versatility of this core and the easy production of the fac-[M(CO)3(H2O)3](+) precursor could explain this interest. The main characteristics of these tricarbonyl complexes are the high substitution stability of the three CO ligands and the corresponding lability of the coordinated water molecules, yielding, via easy exchange of a variety of bi- and tridentate ligands, complexes xof very high kinetic stability. Here, a computational study of different tricarbonyl complexes of Re(I) and Tc(I) was performed using density functional theory. The solvent effect was simulated using the polarizable continuum model. These structures were used as a starting point to investigate the relative stabilities of tricarbonyl complexes with various tridentate ligands. These complexes included an iminodiacetic acid unit for tridentate coordination to the fac-[M(CO)3](+) moiety (M = Re, Tc), an aromatic ring system bearing a functional group (-NO2, -NH2, and -Cl) as a linking site model, and a tethering moiety (a methylene, ethylene, propylene butylene, or pentylene bridge) between the linking and coordinating sites. The optimized complexes showed geometries comparable to those inferred from X-ray data. In general, the Re complexes were more stable than the corresponding Tc complexes. Furthermore, using NH2 as the functional group, a medium length carbon chain, and ortho substitution increased complex stability. All of the bonds involving the metal center presented a closed shell interaction with dative or covalent character, and the strength of these bonds decreased in the sequence Tc-CO > Tc-O > Tc-N.

Biogenesis of the yeast cytochrome bc1 complex.

PubMed

Zara, Vincenzo; Conte, Laura; Trumpower, Bernard L

2009-01-01

The mitochondrial respiratory chain is composed of four different protein complexes that cooperate in electron transfer and proton pumping across the inner mitochondrial membrane. The cytochrome bc1 complex, or complex III, is a component of the mitochondrial respiratory chain. This review will focus on the biogenesis of the bc1 complex in the mitochondria of the yeast Saccharomyces cerevisiae. In wild type yeast mitochondrial membranes the major part of the cytochrome bc1 complex was found in association with one or two copies of the cytochrome c oxidase complex. The analysis of several yeast mutant strains in which single genes or pairs of genes encoding bc1 subunits had been deleted revealed the presence of a common set of bc1 sub-complexes. These sub-complexes are represented by the central core of the bc1 complex, consisting of cytochrome b bound to subunit 7 and subunit 8, by the two core proteins associated with each other, by the Rieske protein associated with subunit 9, and by those deriving from the unexpected interaction of each of the two core proteins with cytochrome c1. Furthermore, a higher molecular mass sub-complex is that composed of cytochrome b, cytochrome c1, core protein 1 and 2, subunit 6, subunit 7 and subunit 8. The identification and characterization of all these sub-complexes may help in defining the steps and the molecular events leading to bc1 assembly in yeast mitochondria.

Macromolecular Transport between the Nucleus and the Cytoplasm: Advances in Mechanism and Emerging Links to Disease

PubMed Central

Tran, Elizabeth J.; King, Megan C.; Corbett, Anita H.

2014-01-01

Transport of macromolecules between the cytoplasm and the nucleus is critical for the function of all eukaryotic cells. Large macromolecular channels termed nuclear pore complexes that span the nuclear envelope mediate the bidirectional transport of cargoes between the nucleus and cytoplasm. However, the influence of macromolecular trafficking extends past the nuclear pore complex to transcription and RNA processing within the nucleus and signaling pathways that reach into the cytoplasm and beyond. At the Mechanisms of Nuclear Transport biennial meeting held from October 18-23, 2013 in Woods Hole, MA, researchers in the field met to report on their recent findings. The work presented highlighted significant advances in understanding nucleocytoplasmic trafficking including how transport receptors and cargoes pass through the nuclear pore complex, the many signaling pathways that impinge on transport pathways, interplay between the nuclear envelope, nuclear pore complexes, and transport pathways, and numerous links between transport pathways and human disease. The goal of this review is to highlight newly emerging themes in nuclear transport and underscore the major questions that are likely to be the focus of future research in the field. PMID:25116306

The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants.

PubMed

Poulet, Axel; Duc, Céline; Voisin, Maxime; Desset, Sophie; Tutois, Sylvie; Vanrobays, Emmanuel; Benoit, Matthias; Evans, David E; Probst, Aline V; Tatout, Christophe

2017-02-01

The linker of nucleoskeleton and cytoskeleton (LINC) complex is an evolutionarily well-conserved protein bridge connecting the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data support its function in nuclear morphology and meiosis, its involvement in chromatin organisation has not been studied in plants. Here, 3D imaging methods have been used to investigate nuclear morphology and chromatin organisation in interphase nuclei of the model plant Arabidopsis thaliana in which heterochromatin clusters in conspicuous chromatin domains called chromocentres. Chromocentres form a repressive chromatin environment contributing to transcriptional silencing of repeated sequences, a general mechanism needed for genome stability. Quantitative measurements of the 3D position of chromocentres indicate their close proximity to the nuclear periphery but that their position varies with nuclear volume and can be altered in specific mutants affecting the LINC complex. Finally, we propose that the plant LINC complex contributes to proper heterochromatin organisation and positioning at the nuclear periphery, since its alteration is associated with the release of transcriptional silencing as well as decompaction of heterochromatic sequences. © 2017. Published by The Company of Biologists Ltd.

Tory II-A: a nuclear ramjet test reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hadley, J.W.

Declassified 28 Nov 1973. The first test reactor in the Pluto program, leading to development of a nuclear ramjet engine, is called Tory II-A. While it is not an actual prototype engine, this reactor embodies a core design which is considered feasible for an engine, and operation of the reactor will provide a test of that core type as well as more generalized values in reactor design and testing. The design of Tory II-A and construction of the reactor and of its test facility are described. Operation of the Tory II-A core at a total power of 160 megawatts, withmore » 800 pounds of air per second passing through the core and emerging at a temperature of 2000 deg F, is the central objective of the test program. All other reactor and facility components exist to support operation of the core, and preliminary steps in the test program itself will be directed primarily toward ensuring attalnment of full-power operation and collection of meaningful data on core behavior during that operation. The core, 3 feet in diameter and 41/2 feet long, will be composed of bundled ceramic tubes whose central holes will provide continuous air passages from end to end of the reactor. These tubes are to be composed of a homogeneous mixture of UO/sub 2/ fuel and BeO moderator, compacted and sintered to achieve high strength and density. (30 references) (auth)« less

Understanding the haling power depletion (HPD) method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Levine, S.; Blyth, T.; Ivanov, K.

2012-07-01

The Pennsylvania State Univ. (PSU) is using the university version of the Studsvik Scandpower Code System (CMS) for research and education purposes. Preparations have been made to incorporate the CMS into the PSU Nuclear Engineering graduate class 'Nuclear Fuel Management' course. The information presented in this paper was developed during the preparation of the material for the course. The Haling Power Depletion (HPD) was presented in the course for the first time. The HPD method has been criticized as not valid by many in the field even though it has been successfully applied at PSU for the past 20 years.more » It was noticed that the radial power distribution (RPD) for low leakage cores during depletion remained similar to that of the HPD during most of the cycle. Thus, the Haling Power Depletion (HPD) may be used conveniently mainly for low leakage cores. Studies were then made to better understand the HPD and the results are presented in this paper. Many different core configurations can be computed quickly with the HPD without using Burnable Poisons (BP) to produce several excellent low leakage core configurations that are viable for power production. Once the HPD core configuration is chosen for further analysis, techniques are available for establishing the BP design to prevent violating any of the safety constraints in such HPD calculated cores. In summary, in this paper it has been shown that the HPD method can be used for guiding the design for the low leakage core. (authors)« less

78 FR 64027 - Preoperational Testing of Emergency Core Cooling Systems for Pressurized-Water Reactors

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-25

...The U.S. Nuclear Regulatory Commission (NRC) is issuing a revision to regulatory guide (RG), 1.79, ``Preoperational Testing of Emergency Core Cooling Systems for Pressurized-Water Reactors.'' This RG is being revised to incorporate guidance for preoperational testing of new pressurized water reactor (PWR) designs.