33 CFR 334.1060 - Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area.

Code of Federal Regulations, 2013 CFR

2013-07-01

... the Oakland Army Base; restricted area. 334.1060 Section 334.1060 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1060 Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area. (a) The area...

33 CFR 334.1060 - Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area.

Code of Federal Regulations, 2012 CFR

2012-07-01

... the Oakland Army Base; restricted area. 334.1060 Section 334.1060 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1060 Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area. (a) The area...

33 CFR 334.1060 - Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the Oakland Army Base; restricted area. 334.1060 Section 334.1060 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1060 Oakland Outer Harbor adjacent to the Oakland Army Base; restricted area. (a) The area...

78 FR 50098 - Notice of Inventory Completion: St. Joseph County Sheriff's Department, Centreville, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-16

....R50000] Notice of Inventory Completion: St. Joseph County Sheriff's Department, Centreville, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The St. Joseph County Sheriff's Department has... control of these human remains should submit a written request to the St. Joseph County Sheriff's...

33 CFR 334.1030 - Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Alameda Facility, Naval Supply Center, Oakland; restricted area. 334.1030 Section 334.1030 Navigation and... RESTRICTED AREA REGULATIONS § 334.1030 Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area. (a) The area. Within 100 feet of the Alameda Facility wharf. (b) The regulations...

33 CFR 334.1030 - Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Alameda Facility, Naval Supply Center, Oakland; restricted area. 334.1030 Section 334.1030 Navigation and... RESTRICTED AREA REGULATIONS § 334.1030 Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area. (a) The area. Within 100 feet of the Alameda Facility wharf. (b) The regulations...

33 CFR 334.1030 - Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Alameda Facility, Naval Supply Center, Oakland; restricted area. 334.1030 Section 334.1030 Navigation and... RESTRICTED AREA REGULATIONS § 334.1030 Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area. (a) The area. Within 100 feet of the Alameda Facility wharf. (b) The regulations...

33 CFR 334.1030 - Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Alameda Facility, Naval Supply Center, Oakland; restricted area. 334.1030 Section 334.1030 Navigation and... RESTRICTED AREA REGULATIONS § 334.1030 Oakland Inner Harbor adjacent to Alameda Facility, Naval Supply Center, Oakland; restricted area. (a) The area. Within 100 feet of the Alameda Facility wharf. (b) The regulations...

78 FR 72911 - Changes in Flood Hazard Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-04

.... Midland Fields, MI 48640. 333 West Ellsworth Street, Midland, MI 48640. Oakland City of Troy (13- The....fema.gov/ City of Troy, Troy, MI 48084. lomc. 500 West Big Beaver Road, Troy, MI 48084. Missouri...:// January 30, 2014 290869 Areas of Lincoln Presiding Troy, MO 63379. www.msc.fema.gov/ County (13-07...

Superfund Record of Decision (EPA Region 5): Cemetery Dump Site, Oakland County, Michigan, September 1985. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1985-09-11

The Cemetery Dump Site is located in Oakland County, Michigan, approximately 35 miles northwest of Detroit. The 4-acre site was once used as a sand and gravel pit and has been backfilled and cleared. Citizen reports allege that approximately 300 to 600 barrels were dumped and buried onsite in the late 1960s or early 1970s. In September 1981, the Michigan Department of Natural Resources excavated and transported offsite approximately 20 to 30 barrel fragments. Analysis of the barrel contents indicated the presence of paint sludges, solvents, PCBs, and oils. This ROD is a source-control remedial action that includes excavation andmore » disposal of approximately 250 drums at an offsite RCRA facility. Total capital cost for the selected remedial action is estimated to be $1,883,261. Any additional remedial actions will be addressed in a separate Record of Decision upon completion of the RI/FS.« less

77 FR 61657 - Indiana Northeastern Railroad Company-Abandonment Exemption-In Branch and St. Joseph Counties, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... Northeastern Railroad Company--Abandonment Exemption--In Branch and St. Joseph Counties, MI Indiana... Branch County, Mich., and milepost 100.69 near Sturgis in St. Joseph County, Mich. (the Line). The Line... .'' Decided: October 2, 2012. By the Board, Rachel D. Campbell, Director, Office of Proceedings. Derrick A...

1988-91 Agreement between Oakland University and the Oakland University Chapter, American Association of University Professors.

ERIC Educational Resources Information Center

American Association of Univ. Professors, Washington, DC.

This document presents the 1988-91 agreement between Oakland University (Michigan) and the Oakland University Chapter of the American Association of University Professors. The following 32 articles are detailed: definitions; recognition; work of the bargaining unit; academic titles; association rights; University management; faculty employment,…

Geostatistical analysis of effective vertical hydraulic conductivity and presence of confining layers in the Shallow Glacial Drift Aquifer, Oakland County, Michigan

USGS Publications Warehouse

Bissell, E.G.; Aichele, Stephen S.

2004-01-01

About 400,000 residents of Oakland County, Mich., rely on ground water for their primary drinking-water supply. More than 90 percent of these residents draw ground water from the shallow glacial drift aquifer. Understanding the vertical hydraulic conductivity of the shallow glacial drift aquifer is important both in identifying areas of ground-water recharge and in evaluating susceptibility to contamination. The geologic environment throughout much of the county, however, is poorly understood and heterogeneous, making conventional aquifer mapping techniques difficult. Geostatistical procedures are therefore used to describe the effective vertical hydraulic conductivity of the top 50 ft of the glacial deposits and to predict the probability of finding a potentially protective confining layer at a given location. The results presented synthesize the available well-log data; however, only about 40 percent of the explainable variation in the dataset is accounted for, making the results more qualitative than quantitative. Most of the variation in the effective vertical hydraulic conductivity cannot be explained with the well-log data currently available (as of 2004). Although the geologic environment is heterogeneous, the quality-assurance process indicated that more than half of the wells in the county’s Wellkey database (statewide database for monitoring drinking-water wells) had inconsistent identifications of lithology.

Faculty Agreement 1983-1985: Oakland University and the Oakland University Chapter of the American Association of University Professors.

ERIC Educational Resources Information Center

American Association of Univ. Professors, Washington, DC.

The collective bargaining agreement between Oakland University and the Oakland University Chapter (370 members) of the American Association of University Professors (AAUP) covering the period March 1, 1983-August 14, 1985 is presented. Items covered in the agreement include: definitions and recognition of AAUP; work of the bargaining unit;…

Superfund Record of Decision (EPA Region 5): Rose Township Cemetery Dump Site, Oakland County, Michigan. (Second remedial action), September 1989. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1989-09-29

The 4-acre Cemetery Dump site is a former sand and gravel pit in Rose Township, Oakland County, Michigan. During the late 1960s or early 1970s, approximately 300 to 600 drums, containing paint sludges, solvents, PCBs, and oils, were illegally dumped and buried onsite. A 1985 Record of Decision (ROD) addressed the soil cleanup which included excavation and offsite disposal of visually-contaminated soil and drum fragments. Soil which was not visually contaminated was sampled and backfilled with clean soil into the excavated areas. Subsequent soil and ground water sampling indicated that zinc contaminants in the ground water exceed the Federal secondarymore » MCL, a nonenforceable standard based on taste and odor, not protection of health. Zinc contamination, however, is most likely a result of monitoring well construction materials. No other contaminants exceed Federal or State environmental standards.« less

Dance and History in Inner-City Oakland

ERIC Educational Resources Information Center

Goodwin, Avilee

2010-01-01

In this article, the author shares how she was able to help young choreographers in East Oakland, California, to find their own creative voices, choose new movement styles and discover dance as a way to express something important and meaningful about their lives through their two-year dance history project. East Oakland School of the Arts (EOSA)…

Effects of urban land-use change on streamflow and water quality in Oakland County, Michigan, 1970-2003, as inferred from urban gradient and temporal analysis

USGS Publications Warehouse

Aichele, Stephen S.

2005-01-01

This apparent contradiction may be caused by the differences in the changes measured in each analysis. The change-through-time approach describes change from a fixed starting point of approximately 1970; the gradient approach describes the cumulative effect of all change up to approximately 2000. These findings indicate that although urbanization in Oakland County results in most of the effects observed in the literature, as evidenced in the gradient approach, relatively few of the anticipated effects have been observed during the past three decades. This relative stability despite rapid land-cover change may be related to efforts to mitigate the effects of development and a general decrease in the density of new residential development. It may also be related to external factors such as climate variability and reduced atmospheric deposition of specific chemicals. 

78 FR 57789 - Establishment and Modification of Class E Airspace; Oakland, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-20

... Class E airspace at Metropolitan Oakland International Airport, Oakland, CA, to accommodate aircraft... at Metropolitan Oakland International Airport. This action also modifies Class E surface airspace.... This improves the safety and management of Instrument Flight Rules (IFR) operations at the airport...

Water Quality in Courtland Creek, East Oakland, California

NASA Astrophysics Data System (ADS)

Bracho, H.; Ahumada, A.; Hernandez, G.; Quintero, D.; Ramirez, J.; Ramirez, L.; Pham, T.; Holt, J.; Johnson, A.; Rubio, E.; Ponce, X.; Medina, S.; Limon, S.

2013-12-01

Courtland Creek is a tributary of the larger East Creek system that runs southeast from the Oakland Hills down to the San Leandro Bay in Oakland, California. In an effort to assess the overall health of Courtland Creek our team conducted a water quality research study. Stream water samples were collected from 4 sites between MacArthur Avenue (describe geographically as not all readers are familiar with Oakland geography) and Thompson Avenue (describe geographically as not all readers are familiar with Oakland geography) at accessible sections of this largely culverted stream. Dissolved oxygen, ammonia, nitrite, nitrate, phosphate, and chlorine concentrations in were measured using wet chemistry procedures. Analysis of collected samples indicates that dissolved oxygen levels in the stream are sufficient for invertebrates, ranging from 5 and 9 parts per million (ppm). Nitrate levels were significantly high, with concentrations ranging from 15 and 40 ppm. Other chemical species associated with waste products--ammonia, nitrite, and phosphate--also were present, but at low concentrations. Small amounts of chlorine also were found in waters of the creek system. The presence of high concentrations of nitrate, together with chlorine, suggests that untreated sewage may be leaking into Courtland Creek at an unidentified location.

33 CFR 165.1190 - Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., Oakland Estuary, Alameda, CA. 165.1190 Section 165.1190 Navigation and Navigable Waters COAST GUARD... § 165.1190 Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA. (a) Location. The following area is a security zone: All navigable waters of the Oakland Estuary, California, from the surface to...

33 CFR 165.1190 - Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., Oakland Estuary, Alameda, CA. 165.1190 Section 165.1190 Navigation and Navigable Waters COAST GUARD... § 165.1190 Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA. (a) Location. The following area is a security zone: All navigable waters of the Oakland Estuary, California, from the surface to...

33 CFR 165.1190 - Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., Oakland Estuary, Alameda, CA. 165.1190 Section 165.1190 Navigation and Navigable Waters COAST GUARD... § 165.1190 Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA. (a) Location. The following area is a security zone: All navigable waters of the Oakland Estuary, California, from the surface to...

33 CFR 165.1190 - Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., Oakland Estuary, Alameda, CA. 165.1190 Section 165.1190 Navigation and Navigable Waters COAST GUARD... § 165.1190 Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA. (a) Location. The following area is a security zone: All navigable waters of the Oakland Estuary, California, from the surface to...

33 CFR 165.1190 - Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., Oakland Estuary, Alameda, CA. 165.1190 Section 165.1190 Navigation and Navigable Waters COAST GUARD... § 165.1190 Security Zone; San Francisco Bay, Oakland Estuary, Alameda, CA. (a) Location. The following area is a security zone: All navigable waters of the Oakland Estuary, California, from the surface to...

Water resources in a rapidly growing region-Oakland County, Michigan

USGS Publications Warehouse

Aichele, Stephen S.

2005-01-01

Despite considerable expansion of urban areas, streamflow characteristics at most sites have not been affected. However, at several sites in areas of the county that are both supplied by ground water and sewered, statistically significant downward trends in low-flow stream discharges have been noted between 1970 and 2003. Stream chemistry, compared to a previous study of county water resources prepared in 1972, has generally improved, with marked decreases in concentrations of nitrogen, phosphorus, and sulfate. Chloride concentrations, however, have increased dramatically in river and lake water across the county. Detectable concentrations of personal-care products, flame retardants, and petroleum fuel compounds were identified at all river sites sampled. 

78 FR 68995 - Safety Zone: Vessel Removal From the Oakland Estuary, Alameda, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-18

...-AA00 Safety Zone: Vessel Removal From the Oakland Estuary, Alameda, CA AGENCY: Coast Guard, DHS. ACTION... waters of the Oakland Estuary just north of the Park Street Bridge in Alameda, CA in support of the Oakland Estuary Closure for the Vessel Removal Project on November 4, 2013 through November 22, 2013. This...

Taxi Regulatory Revision in Oakland and Berkeley, California : Two Case Studies

DOT National Transportation Integrated Search

1983-06-01

A case study was performed of regulatory changes pertaining to taxicabs in Oakland and neighboring Berkeley, CA. In Oakland rates were approximately doubled and limits on the number of taxi permits removed. Companies were allowed to levy a per-trip s...

Human-Centered Design as an Approach for Place-Based Innovation in Public Health: A Case Study from Oakland, California.

PubMed

Vechakul, Jessica; Shrimali, Bina Patel; Sandhu, Jaspal S

2015-12-01

This case study provides a high-level overview of the human-centered design (HCD) or "design thinking" process and its relevance to public health. The Best Babies Zone (BBZ) initiative is a multi-year project aimed at reducing inequities in infant mortality rates. In 2012, BBZ launched pilot programs in three US cities: Cincinnati, Ohio; New Orleans, Louisiana; and Oakland, California. The Alameda County Public Health Department (ACPHD), the lead for the Oakland BBZ site, identified HCD as a promising approach for addressing the social and economic conditions that are important drivers of health inequities. HCD is a process for creating innovative products, services, and strategies that prioritizes the needs of the intended population. ACPHD partnered with the Gobee Group (a social innovation design consultancy) to develop the Design Sprint. The Design Sprint was a 12-week pilot in which 14 professionals from nine organizations used the HCD process to develop concepts for stimulating a vibrant local economy in the Oakland Best Babies Zone. Thirty- to sixty-minute semi-structured interviews were conducted with all 14 individuals involved in the Design Sprint. With the exception of one interview, the interviews were audio-recorded, transcribed, and inductively coded to identify themes. Our experience suggests that HCD can: enhance community engagement; expedite the timeframe for challenge identification, program design, and implementation; and create innovative programs that address complex challenges.

Agreement between Oakland University and the Oakland University Chapter, American Association of University Professors, 1985-88.

ERIC Educational Resources Information Center

American Association of Univ. Professors, Washington, DC.

The collective bargaining agreement between Oakland University and the University's chapter (370 members) of the American Association of University Professors (AAUP) covering the period 1985-1988 is presented. Items covered in the agreement include: definitions and recognition of AAUP, academic titles, AAUP rights, university management,…

Case Study: OCO, Oakland Community Organizations. Strong Neighborhoods, Strong Schools. The Indicators Project on Education Organizing.

ERIC Educational Resources Information Center

Gold, Eva; Simon, Elaine; Brown, Chris

This report describes Oakland, California's Oakland Community Organizations (OCO), which is committed to the improvement of long-term prospects for families and youth living in the Oakland flatlands neighborhoods. These are low- to moderate-income, traditionally African American communities where numerous immigrant groups have settled in recent…

Multiple Landslide-Hazard Scenarios Modeled for the Oakland-Berkeley Area, Northern California

USGS Publications Warehouse

Pike, Richard J.; Graymer, Russell W.

2008-01-01

With the exception of Los Angeles, perhaps no urban area in the United States is more at risk from landsliding, triggered by either precipitation or earthquake, than the San Francisco Bay region of northern California. By January each year, seasonal winter storms usually bring moisture levels of San Francisco Bay region hillsides to the point of saturation, after which additional heavy rainfall may induce landslides of various types and levels of severity. In addition, movement at any time along one of several active faults in the area may generate an earthquake large enough to trigger landslides. The danger to life and property rises each year as local populations continue to expand and more hillsides are graded for development of residential housing and its supporting infrastructure. The chapters in the text consist of: *Introduction by Russell W. Graymer *Chapter 1 Rainfall Thresholds for Landslide Activity, San Francisco Bay Region, Northern California by Raymond C. Wilson *Chapter 2 Susceptibility to Deep-Seated Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike and Steven Sobieszczyk *Chapter 3 Susceptibility to Shallow Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Kevin M. Schmidt and Steven Sobieszczyk *Chapter 4 Landslide Hazard Modeled for the Cities of Oakland, Piedmont, and Berkeley, Northern California, from a M=7.1 Scenario Earthquake on the Hayward Fault Zone by Scott B. Miles and David K. Keefer *Chapter 5 Synthesis of Landslide-Hazard Scenarios Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike The plates consist of: *Plate 1 Susceptibility to Deep-Seated Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike, Russell W. Graymer, Sebastian Roberts, Naomi B. Kalman, and Steven Sobieszczyk *Plate 2 Susceptibility to Shallow Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Kevin M. Schmidt and Steven

Historical vegetation change in Oakland and its implications for urban forest management

Treesearch

David J. Nowak

1993-01-01

The history of Oakland, California's urban forest was researched to determine events that could influence future urban forests. Vegetation in Oakland has changed drastically from a preurbanized area with approximately 2% tree cover to a present tree cover of 19%. Species composition of trees was previously dominated by coast live oak (Quercus agrifolia...

71. MISSISSIPPI, LOWNDES CO. COLUMBUSSTEENS MAP OF LOWNDES COUNTY, 1931 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

71. MISSISSIPPI, LOWNDES CO. COLUMBUS-STEENS MAP OF LOWNDES COUNTY, 1931 Detail of ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Orig. scale: I in. to I mi. Includes Columbus and area NE to steens. Extent: 12 mi. East-West, 9 mi. North-South. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

Oakland Unified School District Community Schools: Supporting Students, Teachers, and Families. Knowledge Brief

ERIC Educational Resources Information Center

John W. Gardner Center for Youth and Their Communities, 2015

2015-01-01

Oakland is home to one of the most demographically diverse populations in the country, with residents of different racial, ethnic, national, linguistic, and other cultural groups. In response to these vast disparities, in 2010, Oakland Unified School District (OUSD) launched an initiative to transform all district schools into full service…

70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, 1931 Detail of ROAD OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Detail of section NW of Columbus (12 mi. N, 9 mi. W), including Plymouth, Waverly, Barton, Buttahatchie R. Orig. scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

78 FR 74007 - Amendment of Class E Airspace; Grand Rapids, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-10

... coordinates for Gerald R. Ford International Airport, formerly known as Kent County International Airport... Gerald R. Ford International Airport, Grand Rapids, MI, formerly called Kent County International Airport... authority as it amends controlled airspace at Gerald R. Ford International Airport, Grand Rapids, MI. List...

76 FR 20071 - Environmental Impact Statement: Oakland and Genesee Counties, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... pavement rehabilitation projects, safety improvement projects, intersection operation projects, and... when the narrow boulevard was found to be equal from a traffic standpoint and acceptable from a design...

Rainfall interception of three trees in Oakland, California

Treesearch

Qingfu Xiao; E. Gregory McPherson

2011-01-01

A rainfall interception study was conducted in Oakland, California to determine the partitioning of rainfall and the chemical composition of precipitation, throughfall, and stemflow. Rainfall interception measurements were conducted on a gingko (Ginkgo biloba) (13.5 m tall deciduous tree), sweet gum (Liquidambar styraciflua) (8...

Reflections of a Former Oakland Public School Parent.

ERIC Educational Resources Information Center

Abron, JoNina M.

1997-01-01

Shares the perspectives of a black parent, herself a teacher at a school founded by the Black Panthers, whose daughter was educated in the public schools of Oakland (California) and Kalamazoo (Michigan) between 1986 and 1996. Many negative experiences, some centered around language, are described. (SLD)

33 CFR 334.1050 - Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of...

Code of Federal Regulations, 2011 CFR

2011-07-01

... the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. 334..., DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1050 Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. (a...

33 CFR 334.1050 - Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of...

Code of Federal Regulations, 2014 CFR

2014-07-01

... the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. 334..., DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1050 Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. (a...

33 CFR 334.1050 - Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of...

Code of Federal Regulations, 2010 CFR

2010-07-01

... the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. 334..., DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1050 Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. (a...

33 CFR 334.1050 - Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of...

Code of Federal Regulations, 2012 CFR

2012-07-01

... the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. 334..., DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1050 Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. (a...

33 CFR 334.1050 - Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of...

Code of Federal Regulations, 2013 CFR

2013-07-01

... the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. 334..., DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1050 Oakland Outer Harbor adjacent to the Military Ocean Terminal, Bay Area, Pier No. 8 (Port of Oakland Berth No. 10); restricted area. (a...

Vocational Education: Cause or Cure for Youth Unemployment? A Report to the Citizens of Oakland, Calif.

ERIC Educational Resources Information Center

National Association for the Advancement of Colored People Legal Defense and Educational Fund, New York, NY.

This is an evaluation report on the vocational education program in the majority black school system of Oakland, California. An overview of the employment problems in Oakland and a description of the report's preparation are contained in an introduction. Chapter two examines the relationship of the vocational education program to planning,…

Examples of Integrated Academic and Vocational Curriculum from High School Academies in the Oakland Unified School District.

ERIC Educational Resources Information Center

de Leeuw, David; And Others

This report provides examples of curricular integration produced by teachers in three career academies in Oakland, California. It describes ways in which academic and vocational teachers have created explicit connections between their separate subjects. Following a preface, "Oakland Academies Magnet Programs--An Overview" (Allie…

Making Curiosity Accessible: Lynne Cutler--Oakland Public Library

ERIC Educational Resources Information Center

Library Journal, 2004

2004-01-01

Lynne Cutler's driving force is the intense curiosity that led her to audit 24 additional courses while studying librarianship. It is what drives her to make Oakland Public Library's services available to those with disabilities, so that everyone can have "access to all the things in life I treasure, like books, words, music, art,…

Digging West Oakland : what archaeologists found under the Cypress Freeway.

DOT National Transportation Integrated Search

2005-01-01

In 1989, the Loma Prieta earthquake destroyed 1.25 miles of the double-deck Cypress Freeway in Oakland, California. In realigning and rebuilding the freeway, the California Department of Transportation (Caltrans) undertook to identify any important a...

Geologic Map and Map Database of the Oakland Metropolitan Area, Alameda, Contra Costa, and San Francisco Counties, California

USGS Publications Warehouse

Graymer, R.W.

2000-01-01

Introduction This report contains a new geologic map at 1:50,000 scale, derived from a set of geologic map databases containing information at a resolution associated with 1:24,000 scale, and a new description of geologic map units and structural relationships in the mapped area. The map database represents the integration of previously published reports and new geologic mapping and field checking by the author (see Sources of Data index map on the map sheet or the Arc-Info coverage pi-so and the textfile pi-so.txt). The descriptive text (below) contains new ideas about the Hayward fault and other faults in the East Bay fault system, as well as new ideas about the geologic units and their relations. These new data are released in digital form in conjunction with the Federal Emergency Management Agency Project Impact in Oakland. The goal of Project Impact is to use geologic information in land-use and emergency services planning to reduce the losses occurring during earthquakes, landslides, and other hazardous geologic events. The USGS, California Division of Mines and Geology, FEMA, California Office of Emergency Services, and City of Oakland participated in the cooperative project. The geologic data in this report were provided in pre-release form to other Project Impact scientists, and served as one of the basic data layers for the analysis of hazard related to earthquake shaking, liquifaction, earthquake induced landsliding, and rainfall induced landsliding. The publication of these data provides an opportunity for regional planners, local, state, and federal agencies, teachers, consultants, and others outside Project Impact who are interested in geologic data to have the new data long before a traditional paper map could be published. Because the database contains information about both the bedrock and surficial deposits, it has practical applications in the study of groundwater and engineering of hillside materials, as well as the study of geologic hazards and

School-Based Intervention for Nutrition Promotion in Mi Yun County, Beijing, China: Does a Health-Promoting School Approach Improve Parents' Knowledge, Attitudes and Behaviour?

ERIC Educational Resources Information Center

Wang, Dongxu; Stewart, Donald; Chang, Chun

2016-01-01

Purpose: The purpose of this paper is to assess whether the school-based nutrition programme using the health-promoting school (HPS) framework was effective to improve parents' knowledge, attitudes and behaviour (KAB) in relation to nutrition in rural Mi Yun County, Beijing. Design/methodology/approach: A cluster-randomised intervention trial…

78 FR 65380 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... the University of Michigan, Ann Arbor, MI. The human remains were removed from Alpena, Isabella, Grand... removed from the Devil River Mound site (20AL1) in Alpena County, MI. A resident of Ossineke, MI...

The Impact of Oakland Freedom School's Summer Youth Program on the Psychosocial Development of African American Youth

ERIC Educational Resources Information Center

Bethea, Sharon L.

2012-01-01

The present investigation considers the program outcomes of one community youth project, Leadership Excellence Inc., Oakland Freedom Schools. Oakland Freedom Schools are culturally relevant 6-week summer Language Arts enrichment programs for primarily inner-city African American youth aged 5 to 14 years. In this study, 79 African American youth…

Spatial and temporal variations in land development and impervious surface creation in Oakland County, Michigan, 1945-2005

NASA Astrophysics Data System (ADS)

Aichele, Stephen S.; Andresen, Jeffrey A.

2013-04-01

SummaryImpervious surface has been recognized as a key indicator of watershed health and function. The rapid expansion of impervious surface associated with periurban development following the Second World War resulted in concerns that impervious surface would alter flow characteristics, water quality, sediment, and stream morphology. These effects have been documented in studies across many disciplines. Unfortunately, impervious surface is difficult to measure directly, and other forms of land-use data are often substituted as surrogates. This paper highlights the shortcomings in land-use data, particularly parcel-based land-use data, as a surrogate for impervious surface in a periurban environment. Periurban development has changed substantially in the last several decades. This study investigates changes in the form of periurban development in Oakland County, Michigan, from 1945 to 2005, with an emphasis on the accumulation of impervious surface. We first evaluate patterns in the sizes of parcels being developed to residential uses. Using an impervious surface map derived from aerial imagery, we then calculate amount of impervious surface created by different forms of development, both in parcels of similar sizes developed at different times, and across parcel sizes for the period of the study. The results indicate substantial variability in impervious surface within periurban residential development, from 5.4% of parcel area to 25.4% of total parcel area depending on parcel size. Even within relatively specific categories (for example, residential parcels less than 743 square metre) impervious surface varied between 18.5% and 34.6% of the parcel area between 1945 and 2000. Since 1980, the trend has been toward larger parcel sizes with lower impervious surface ratios. The overall effect is that land is being developed at a rate substantially greater than the rate impervious surface is being created. The bias created by the trend to larger parcel sizes with

Oakland Crack Task Force: A Portrait of Community Mobilization.

ERIC Educational Resources Information Center

Sutton, Donald; Baker, Ralph F.

The Oakland Crack Task Force (OCTF) was created by concerned citizens to combat the problems caused by crack cocaine and ensure the future existence of the family, especially the black family, using community resources and no outside funding. Goals are to educate the community about crack; identify and access prevention, intervention, treatment,…

Teaching and Learning in Oakland Community Schools. Knowledge Brief

ERIC Educational Resources Information Center

John W. Gardner Center for Youth and Their Communities, 2015

2015-01-01

This brief is part of a series that shares findings from a research collaboration between the John W. Gardner Center for Youth and Their Communities at Stanford University and Oakland Unified School District (OUSD) focused on understanding implementation of the community school model in the district. Community schools aim to address students'…

Organizational Structures to Support Oakland Community Schools. Knowledge Brief

ERIC Educational Resources Information Center

John W. Gardner Center for Youth and Their Communities, 2015

2015-01-01

This brief is part of a series that shares findings from a research collaboration between the John W. Gardner Center for Youth and Their Communities at Stanford University and Oakland Unified School District (OUSD) focused on understanding implementation of the community school model in the district. This brief highlights findings related to…

Airspace Analysis for Phase II of the Regional Airport Plan Update Program.

DTIC Science & Technology

1979-03-01

Base; and Hamilton, Sonoma County , and Napa County Airports) is comparable in terms of airspace capacity. Note that if the aircraft redistributed to the...Oakland ARTCC (Oakland Center) Napa County Airport Sonoma County Airport (at Santa Rosa) Stockton Approach Control Livermore Municipal Airport... Sonoma County Airport The ATC Tower located at these airports exercises control of aircraft within the Airport Traffic Areas. Rules of aircraft

Interview with Violet Oaklander, Author of "Windows to Our Children."

ERIC Educational Resources Information Center

Campbell, Chari A.

1993-01-01

Presents interview with Dr. Violent Oaklander, experienced child therapist and author of "Windows to Our Children: A Gestalt Therapy with Children and Adolescents." Discusses critical components of successful therapy with children. Explains how developmental stage of child affects therapeutic process, and provides specific examples of working with…

Properties of Anchor Rods Removed from San Francisco-Oakland Bay Bridge

DOT National Transportation Integrated Search

2015-08-01

In March 2013, the construction contractor for the new self-anchored suspension bridge between San Francisco and Oakland, California, tensioned the threaded rods between the bearings/shear keys and the concrete pier cap. Within days of completion, it...

33 CFR 165.1187 - Security Zones; Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay...

Code of Federal Regulations, 2011 CFR

2011-07-01

... Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay, California. 165.1187 Section 165.1187... Limited Access Areas Eleventh Coast Guard District § 165.1187 Security Zones; Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay, California. (a) Location. All waters extending...

33 CFR 165.1187 - Security Zones; Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay...

Code of Federal Regulations, 2010 CFR

2010-07-01

... Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay, California. 165.1187 Section 165.1187... Limited Access Areas Eleventh Coast Guard District § 165.1187 Security Zones; Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay, California. (a) Location. All waters extending...

77 FR 38472 - Amendment of Class D Airspace; Pontiac, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-28

...-1142; Airspace Docket No. 11-AGL-22] Amendment of Class D Airspace; Pontiac, MI AGENCY: Federal... Class D airspace within the Pontiac, MI, area by changing the name of the airport from [[Page 38473... County International Airport and adjusting the geographic coordinates within Class D airspace to coincide...

A Tale of Two Cities: Access to Care and Services Among African-American Transgender Women in Oakland and San Francisco

PubMed Central

Cruz, Taylor M.; Iwamoto, Mariko; Sakata, Maria

2015-01-01

Abstract Purpose: The San Francisco Bay Area attracts people from all over the country due to the perception of lesbian, gay, bisexual, and transgender (LGBT) acceptance and affirmation. African-American transgender women are severely marginalized across society and as such have many unmet health and social service needs. This study sought to quantitatively assess unmet needs among African-American transgender women with a history of sex work by comparing residents of Oakland versus San Francisco. Methods: A total of 235 African-American transgender women were recruited from San Francisco (n=112) and Oakland (n=123) through community outreach and in collaboration with AIDS service organizations. Participants were surveyed regarding basic, health, and social needs and HIV risk behaviors. Pearson Chi-squared tests and a linear regression model examined associations between city of residence and unmet needs. Results: While participants from both cities reported unmet needs, Oakland participants had a greater number of unmet needs in receiving basic assistance, mental health treatment, and health care services. Oakland participants also reported less transgender community identification but higher social support from the family. Conclusion: These findings demonstrate the enormity of African-American transgender women's needs within the Bay Area. Greater resources are needed for social service provision targeting this marginalized group of people, particularly in Oakland. PMID:26788672

Beyond the Factory Model: Oakland Teachers Learn How to Blend

ERIC Educational Resources Information Center

Jacobs, Joanne

2014-01-01

This article describes an Oakland Unified schools program of "blended learning" that is designed to reach students who are academically all over the map. Blended learning combines brick-and-mortar schooling with online education "with some element of student control over time, place, path, and/or pace" of learning. The program…

ETV REPORT: REMOVAL OF ARSENIC IN DRINKING WATER - PALL CORPORATION MICROZA. MICROFILTRATION SYSTEM

EPA Science Inventory

Verification testing of the Pall Corporation Microza. Microfiltration System for arsenic removal was conducted at the Oakland County Drain Commissioner (OCDC) Plum Creek Development well station located in Oakland County, Michigan from August 19 through October 8, 2004. The sourc...

77 FR 5170 - Amendment of Class D Airspace; Jackson, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-02

...-1143; Airspace Docket No. 11-AGL-23] Amendment of Class D Airspace; Jackson, MI AGENCY: Federal... Class D airspace within the Jackson, MI, area by changing the name of the airport from Jackson County... Class D airspace to coincide with the FAA's aeronautical database. This is an administrative change and...

Integrated Services and Supports in Oakland Community Schools. Knowledge Brief

ERIC Educational Resources Information Center

John W. Gardner Center for Youth and Their Communities, 2015

2015-01-01

This brief is part of a series that shares findings from a research collaboration between the John W. Gardner Center for Youth and Their Communities at Stanford University and Oakland Unified School District (OUSD) focused on understanding implementation of the community school model in the district. This brief highlights findings related to…

5. GENERAL VIEW SHOWING ORIGINAL SETTING AT WHARFSIDE WITH CONTAINERIZED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. GENERAL VIEW SHOWING ORIGINAL SETTING AT WHARFSIDE WITH CONTAINERIZED FREIGHT LOADING EQUIPMENT AT PORT OF OAKLAND FACILITY - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

11. RAILROAD BRIDGE MISSISSIPPI, MONROE CO., AMORY Onehalf mi. S ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. RAILROAD BRIDGE MISSISSIPPI, MONROE CO., AMORY One-half mi. S of MS. 6, 1.5 mi. NW of Amory. Aerial view, close-up, from SE of St. Louis and San Francisco RR bridge. David Kaminsky, Architectural Photography, photographer, August 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

A Creek to Bay Biological Assessment in Oakland, California

NASA Astrophysics Data System (ADS)

Ahumada, E.; Ramirez, N.; Lopez, A.; Avila, M.; Ramirez, J.; Arroyo, D.; Bracho, H.; Casanova, A.; Pierson, E.

2011-12-01

In 2007, the Surface Water Ambient Monitoring Program (SWAMP) assessed the impact of trash on water quality in the Peralta Creek which is located in the Fruitvale district of Oakland, CA. This 2011 follow-up study will take further steps in evaluating the physical and biological impacts of pollution and human development on Peralta Creek and in the San Leandro Bay, where the Creek empties into the larger San Francisco Bay estuary. This study will utilize two forms of biological assessment in order to determine the level of water quality and ecosystem health of Peralta Creek and San Leandro Bay in Oakland, California. A Rapid Bioassesment Protocal (RBP) will be used as the method of biological assessment for Peralta Creek. RBP uses a biotic index of benthic macroinvertebrates to provide a measure of a water body's health. Larval trematodes found in two mud snails (Ilynassa obsoleta and Cerithidea californica) will be used to evaluate the health of the San Leandro Bay. Due to the complex life cycle of trematodes, the measure of trematode diversity and richness in host species serves as an indicator of estuarine health (Huspeni 2005). We have completed the assessment of one section of Peralta Creek, located at 2465 34th Avenue, Oakland, CA 94601. Abundance results indicate a moderately healthy creek because there were high levels of pollution tolerant benthic macroinvertebrates. The tolerant group of benthic macroinvertebrates includes such organisms as flatworms, leeches, and scuds. This is possibly due to this section of the creek being pumped up to the surface from culverts impacting the macroinvertebrate's life cycle. Another contributing factor to creek health is the amount of organic debris found in the creek, which inhibits the flow and oxygenation of the water, allowing for more pollution tolerant aquatic insects to persist. Further investigation is being conducted to fully assess the Peralta Creek watershed; from the preliminary results one can surmise that

Spatial variations of particulate matter and air toxics in communities adjacent to the Port of Oakland.

PubMed

Fujita, Eric M; Campbell, David E; Arnott, W Patrick; Lau, Virginia; Martien, Philip T

2013-12-01

The Bay Area Air Quality Management District (BAAQMD) sponsored the West Oakland Monitoring Study (WOMS) to provide supplemental air quality monitoring that will be used by the BAAQMD to evaluate local-scale dispersion modeling of diesel emissions and other toxic air contaminants for the area within and around the Port of Oakland. The WOMS was conducted during two seasonal periods of 4 weeks in summer 2009 and winter 2009/2010. Monitoring data showed spatial patterns of pollutant concentrations that were generally consistent with proximity to vehicle traffic. Concentrations of directly emitted pollutants were highest on heavily traveled roads with consistently lower concentrations away from the roadways. Pollutants that have higher emission rates from diesel trucks (nitric oxide, black carbon) tended to exhibit sharper gradients than pollutants that are largely associated with gasoline vehicles, such as carbon monoxide and volatile organic compounds, including benzene, toluene, ethylbenzene, and xylenes (BTEX). BTEX concentrations in West Oakland were similar to those measured at the three air toxics monitoring network sites in the Bay Area (San Francisco, Fremont, and San Jose). Aldehyde levels were higher in Fremont and San Jose than in West Oakland, reflecting greater contributions from photo-oxidation of hydrocarbons downwind of the Bay Area. A 2005 modeling-based health risk assessment of diesel particulate matter concentrations is consistent with aerosol carbon concentrations measured during the WOMS after adjusting for recent mitigation measures and improved estimates of heavy-duty truck traffic volumes.

Violent Injury and Neighborhood Racial/Ethnic Diversity in Oakland, California.

PubMed

Berezin, Joshua; Gale, Sara; Nuru-Jeter, Amani; Lahiff, Maureen; Auerswald, Colette; Alter, Harrison

2017-12-01

Racial and ethnic segregation has been linked to a number of deleterious health outcomes, including violence. Previous studies of segregation and violence have focused on segregation between African Americans and Whites, used homicide as a measure of violence, and employed segregation measures that fail to take into account neighborhood level processes. We examined the relationship between neighborhood diversity and violent injury in Oakland, California. Violent injuries from the Alameda County Medical Center Trauma Registry that occurred between 1998 and 2002 were geocoded. A local measure of diversity among African American, White, Hispanic, and Asian populations that captured interactions across census block group boundaries was calculated from 2000 U.S. Census data and a Geographic Information System. The relationship between violent injuries and neighborhood level of diversity, adjusted for covariates, was analyzed with zero-inflated negative binomial regression. There was a significant and inverse association between level of racial and ethnic diversity and rate of violent injury (IRR 0.30; 95% CI: 0.13-0.69). There was a similar relationship between diversity and violent injury for predominantly African American block groups (IRR 0.23; 95% CI: 0.08-0.62) and predominantly Hispanic block groups (IRR 0.08; 95% CI: 0.01-0.76). Diversity was not significantly associated with violent injury in predominantly White or Asian block groups. Block group racial and ethnic diversity is associated with lower rates of violent injury, particularly for predominantly African American and Hispanic block groups.

Superfund Record of Decision Amendment (EPA Region 5): Springfield Township Dump, Davisburg, MI, June 10, 1998

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This decision document presents the amendments to the remedial action for the Springfield Township Dump site, Oakland County, Michigan. The amended remedial action changes the selected method of addressing PCB-laden soils and also changes certain soil and groundwater cleanup standards previously selected in the 1990 Record of Decision (ROD) to reflect current state standards: The groundwater and soil vapor extraction and treatment systems and the arsenic and lead groundwater cleanup standards identified as part of the selected remedy in the 1990 ROD and in the 1993 Explanation of Significant Difference remain unchanged.

Arsenic in ground water in Tuscola County, Michigan

USGS Publications Warehouse

Haack, Sheridan K.; Rachol, Cynthia M.

2000-01-01

Previous studies of ground-water resources in Michigan by the Michigan Department of Community Health (MDCH), the Michigan Department of Environmental Quality (MDEQ), and the U.S. Geological Survey (USGS) indicate that in several counties in the southeastern part of the State the concentrations of arsenic in ground water may exceed the U.S. Environmental Protection Agency (USEPA) maximum contaminant level (MCL) of 50 micrograms per liter [µg/L]. This MCL was established in 1986. The Safe Drinking Water Act, as amended in 1996, requires USEPA to revise this standard in 2000. In June 2000, the USEPA proposed a revised MCL of 5 µg/L. In 1996, the USGS, in cooperation with the MDEQ and the Health Departments of Genesee, Huron, Lapeer, Livingston, Oakland, Sanilac, Shiawassee, Tuscola and Washtenaw counties, began a study of the factors controlling arsenic occurrence and concentrations in ground water in southeastern Michigan. This study is one of four USGS Drinking Water Initiative projects throughout the United States.

Particulate Concentration Levels in Chinatown, Oakland, California

NASA Astrophysics Data System (ADS)

Chen, B.; Yeung, A.; Yu, J. F.

2007-12-01

Chinatown is located near the center of the busy business district of downtown Oakland, California. It is one of the most inhabited and congested areas in the City of Oakland, averaging 4,000 vehicles and 3,000 pedestrians per hour at a key intersection in the center of the neighborhood. Particles produced by automobiles and construction can settle into the bronchi of lungs and induce asthma attacks, irritate cardiovascular tissue, and possibly lead to lung cancer and death. Particulate pollution is a serious problem that is estimated to cause between 20,000 and 50,000 deaths per year in the US alone. Hence, evaluation of the air quality of the Chinatown neighborhood is important, because it helps to address issues that are of great concern to residents of the area. The primary goal of our project was to measure particulate concentration levels at various intersections in Oakland's Chinatown to determine if the air quality met U.S. EPA standards, and to take note of any trends that may occur over a period of months. We were primarily concerned with particles that are 2.5 micrometers diameter and smaller, as smaller particles are easily inhaled and directly affect the respiratory system. We were interested in identifying any intersections that may have had significantly higher levels than other intersections. Using a map of Chinatown, we chose 12 intersections and made measurements at these points over the course of six months, beginning in February and ending in July of 2007. Particulate matter measurements were made using a FLUKE 893 Particle Counter. Measurements recorded on the first day of our study, February 4, 2007, which was the day of an annual street festival, yielded the highest values for particulate matter concentration in our dataset. This was followed by a significant drop in concentration the following week, and then a gradual increase of concentration as the months progressed. No one location yielded values significantly higher than any other, and

Documents related to Proposed Class II injection well permit modification MI-147-2D-0014

EPA Pesticide Factsheets

Public notice for proposed modification of permit number MI-147-2D-0014 (St. Clair County, MI). The proposed modification would change the injection zone, the construction, and reduce the injection pressure.

Characterizing Soil Lead Contamination Near Streams in Oakland, California

NASA Astrophysics Data System (ADS)

Tanouye, D.

2017-12-01

Lead (Pb) contamination of soils, groundwater, and surface waters is a major concern because of the potential health risks related to accumulation of high levels of lead in blood. This is a pervasive issue in many low-income neighborhoods throughout the United States, and is documented to be particularly acute in West Oakland, California. The fate and transport of lead in the environment is largely dependent on how it will bind to various solids and compounds in solution. These adsorption mechanisms are a principal aspect of metal dissolution and chemical speciation. Stream channels are natural drainage areas for urban runoff, and may represent a hot spot for increased levels of lead. This study evaluates the environmental conditions at 15 sites near streams in West Oakland using in-situ soil sampling with the handheld X-Ray Fluorescence (XRF) analyzer to measure concentrations of lead in soil. Results from this study suggest that the levels of lead in soils near stream channels are generally lower than the regional regulatory screening level of 80 milligrams per kilogram (mg/kg), but the highest concentrations are found near stream banks. The spatial distribution can be explained by a contaminant transport process related to the presence of fluvial channels.

31. OBLIQUE AERIAL VIEW TO THE NORTHEAST, SHOWING THE FEDERAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. OBLIQUE AERIAL VIEW TO THE NORTHEAST, SHOWING THE FEDERAL CHANNEL IN RELATION TO DOWNTOWN OAKLAND AND LAKE MERRITT. Date and time of photography "12-9-98 10:54." - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

GIS Spatial Analysis of Water Quality at Courtland Creek in Oakland, California

NASA Astrophysics Data System (ADS)

Matias, F.; Perez, L.; Martinez, E.; Rivera Soto, E.; McDonald, K.; Garcia, D.; Ruiz, I.

2015-12-01

Courtland Creek is a channelized stream that traverses residential and industrial sections of East Oakland, California. Segments of the creek are exposed on the surface and have been designated as City of Oakland park land. Since 2012, the quality of creek waters has been monitored through measurement and analysis of nutrient and other possible contaminant levels in samples collected in these exposed segments. Throughout the three-year period during which monitoring efforts have been undertaken, high concentration levels of nitrate have been observed. The primary aim of our research is to gain an overall indication of creek health in relation to its surrounding environment through the use of Geographic Information Systems (GIS) analysis of nutrient concentrations at the four sites. Investigating the relationship between Courtland Creek and the environmental factors influencing its health will enable us to develop a better sense of the actions that can be taken by the City of Oakland to create sustainable park land and healthy communities. During the summer of 2015, our group continued to monitor levels of ammonia, phosphate and nitrate at four different sites along the creek, and benthic macroinvertebrates were sampled at one of these sites. Preliminary analysis of benthic macroinvertebrate data indicates that Courtland Creek is in poor health ecologically. Nitrate concentration levels measured during the study period were lower than those detected in previous years but still indicate inputs other than those associated with natural processes. The high nitrate concentration levels may be the result of human and animal waste pollution, as supported by data obtained during a recent Environmental Protection Agency (EPA) - led E. coli survey that included the watershed within which Courtland Creek is situated.

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

78 FR 40076 - Proposed Establishment and Modification of Class E Airspace; Oakland, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-03

...; Oakland, CA AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to establish Class E airspace extending upward from 700 feet above... instrument approach procedures at the airport. This action would also modify Class E surface airspace...

76 FR 9402 - Michigan Air-Line Railway Co.-Abandonment Exemption-in Oakland County, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-17

... offer of financial assistance (OFA) under 49 CFR 1152.27(b)(2) will be due no later than 10 days after... fee. See 49 CFR 1002.2(f)(25). All interested persons should be aware that, following abandonment of... be accompanied by a $250 filing fee. See 49 CFR 1002.2(f)(27). All filings in response to this notice...

76 FR 43743 - Michigan Air-Line Railway Co.-Abandonment Exemption-in Oakland County, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

.... Any offer of financial assistance (OFA) under 49 CFR 1152.27(b)(2) will be due no later than 10 days...,500 filing fee. See 49 CFR 1002.2(f)(25). All interested persons should be aware that, following... banking under 49 CFR 1152.29 will be due no later than August 10, 2011. Each trail use request must be...

4. EAST FACE. VEGETATIVE SCREENS BUFFER THE BUILDING FROM INDUSTRIAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. EAST FACE. VEGETATIVE SCREENS BUFFER THE BUILDING FROM INDUSTRIAL AREAS TO THE SOUTH OF OARB. - Oakland Army Base, General Purpose Administration Building, Chungking & Algiers Streets, Oakland, Alameda County, CA

1. CONTEXT VIEW OF BUILDING 746 FROM EAST K STREET, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. CONTEXT VIEW OF BUILDING 746 FROM EAST K STREET, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Gymnasium-Cafeteria-Theater, East K Street between Eleventh & Twelfth Streets, Oakland, Alameda County, CA

18. GENERAL VIEW, LOOKING NORTHEAST TO SOUTHWEST, SHOWING RELATIONSHIP TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. GENERAL VIEW, LOOKING NORTHEAST TO SOUTHWEST, SHOWING RELATIONSHIP TO OFFICE AREA TO STAIRS - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

4. NORTH SIDE, OBLIQUE VIEW, FROM INTERSECTION OF G AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. NORTH SIDE, OBLIQUE VIEW, FROM INTERSECTION OF G AND 5TH STREETS, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Storehouse, Between G & H Streets, & Fifth & Sixth Streets, Oakland, Alameda County, CA

2. EXTERIOR OBLIQUE VIEW OF BUILDING 746 FROM EAST K ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. EXTERIOR OBLIQUE VIEW OF BUILDING 746 FROM EAST K STREET, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Gymnasium-Cafeteria-Theater, East K Street between Eleventh & Twelfth Streets, Oakland, Alameda County, CA

1. WEST AND NORTH (OBLIQUE VIEW) SIDES, FROM ACROSS 4TH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. WEST AND NORTH (OBLIQUE VIEW) SIDES, FROM ACROSS 4TH STREET, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Warehouse, North of A Street, between Fourth & Maritime Streets, Oakland, Alameda County, CA

4. INTERIOR VIEW STEAM EQUIPMENT AND OFFICE OF BUILDING 842, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. INTERIOR VIEW STEAM EQUIPMENT AND OFFICE OF BUILDING 842, LOOKING EAST-NORTHEAST. - Oakland Naval Supply Center, Academic Instruction Building, Off East K Street near Twelfth Street, Oakland, Alameda County, CA

Constructing Identity through Negotiation for Cambodian Adult English Language Learners in East Oakland

ERIC Educational Resources Information Center

Washington, Brad D.

2016-01-01

This study engages with a participatory oral history project that explores 3 themes. First, Cambodian participants included in the study will narrate from their perspectives how the evolution of social engagement and identity among African American and Cambodian refugee communities residing in historically Black neighborhoods of Oakland,…

16. DETAIL VIEW OF NORTHWEST SIDE LOADING DOCKS, SHOWING SUSPENDER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. DETAIL VIEW OF NORTHWEST SIDE LOADING DOCKS, SHOWING SUSPENDER BARS AND ORIGINAL SHIPLAP SIDING - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

17. GENERAL VIEW, LOOKING NORTHEAST TO SOUTHWEST, SHOWING RELATIONSHIP OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. GENERAL VIEW, LOOKING NORTHEAST TO SOUTHWEST, SHOWING RELATIONSHIP OF TRUSS TO COLUMNS AND BAYS - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

22. SIDE WALL CONSTRUCTION, NORTH TRAINING WALL, LOOKING WEST FROM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. SIDE WALL CONSTRUCTION, NORTH TRAINING WALL, LOOKING WEST FROM THE SAME POINT AS VIEW NO. 21. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

Community Schools as Urban District Reform: Analyzing Oakland's Policy Landscape through Oral Histories

ERIC Educational Resources Information Center

Trujillo, Tina M.; Hernández, Laura E.; Jarrell, Tonja; Kissell, René

2014-01-01

The purpose of this article is to investigate the multiple political histories that have coalesced to produce support for or resistance to the Oakland Unified School District's full-service community schools policy. It analyzes oral history interview data from eight stakeholders who represent the district's major constituencies to explore the…

2. BUILDING 324, WEST SIDE, FROM NEAR SOUTHWEST CORNER OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. BUILDING 324, WEST SIDE, FROM NEAR SOUTHWEST CORNER OF BUILDING 322, LOOKING EAST. - Oakland Naval Supply Center, Commanding Officers Residences, Between E & F Streets, West of Fourth Street, Oakland, Alameda County, CA

12. VIEW OF CANOPY OVER NORTHWEST LOADING PLATFORM, RUNNING NEARLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. VIEW OF CANOPY OVER NORTHWEST LOADING PLATFORM, RUNNING NEARLY THE ENTIRE LENGTH OF THE BUILDING - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

14. GENERAL OBLIQUE VIEW OF WEST CORNER OF SHED, OBSTRUCTED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. GENERAL OBLIQUE VIEW OF WEST CORNER OF SHED, OBSTRUCTED BY LATE METAL BUILDING, LOOKING EAST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

28. EAST END OF THE NORTH TRAINING WALL AT THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

28. EAST END OF THE NORTH TRAINING WALL AT THE FISHING PIER, FROM THE WATER, LOOKING NORTH-NORTHEAST. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

1. WEST SIDE AND ENTRY, FROM ACROSS 3RD STREET, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. WEST SIDE AND ENTRY, FROM ACROSS 3RD STREET, LOOKING EAST. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

2. SOUTH SIDE, FROM PARK ACROSS PARKING LOT/F STREET, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. SOUTH SIDE, FROM PARK ACROSS PARKING LOT/F STREET, LOOKING NORTH. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

15. MASONRY DETAIL NO. 1, NORTH TRAINING WALL, LOOKING DOWN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. MASONRY DETAIL NO. 1, NORTH TRAINING WALL, LOOKING DOWN UPON THE WALL SURFACE FROM THE ADJACENT RIPRAP. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

5. INTERIOR, STORAGE AREA AT WESTERN END, FROM SOUTH OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. INTERIOR, STORAGE AREA AT WESTERN END, FROM SOUTH OF LOADING DOCK WELL, LOOKING NORTH. - Oakland Naval Supply Center, Coffee Roasting Plant, East of Fourth Street, between J & K, Oakland, Alameda County, CA

6. GENERAL WIDE VIEW SHOWING EAST (SOUTHEAST) SIDE, LOOKING WEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. GENERAL WIDE VIEW SHOWING EAST (SOUTHEAST) SIDE, LOOKING WEST ACROSS TURNING BASIN; FREIGHTER LOADING IN FOREGROUND - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

3. OBLIQUE GENERAL VIEW SHOWING EAST CORNER OF SHED, WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. OBLIQUE GENERAL VIEW SHOWING EAST CORNER OF SHED, WITH RAILROAD TRACKS PASSING UNDER DERRICK ALONG WHARF - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

25. VIEW OF EARTHQUAKEDAMAGED TRUSS MEMBER AT #070, SUPPORTED BY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. VIEW OF EARTHQUAKE-DAMAGED TRUSS MEMBER AT #070, SUPPORTED BY TEMPORARY BRACING, LOOKING NORTHEAST TO SOUTHWEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

13. DETAIL VIEW OF LOADING DOCK CANOPY, SHOWING TWIN TIMBER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. DETAIL VIEW OF LOADING DOCK CANOPY, SHOWING TWIN TIMBER SUPPORT MEMBERS AND SUSPENDER BAR MOUNTING HARDWARE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

Rockfaced, coursed ashlar wing wall on the southwest corner of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Rock-faced, coursed ashlar wing wall on the southwest corner of the bridge, facing west. - Oakland Avenue Viaduct, Oakland Avenue spanning U.S. Route 62 (State Route 2302) & Pine Run, Sharon, Mercer County, PA

4. CENTER COURTYARD, FROM WEST SIDE OF SOUTHEAST PORTION OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. CENTER COURTYARD, FROM WEST SIDE OF SOUTHEAST PORTION OF BUILDING, LOOKING WEST. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

7. INTERIOR, KITCHEN SOUTH OF CENTRAL EASTWEST CORRIDOR, FROM ENTRY, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. INTERIOR, KITCHEN SOUTH OF CENTRAL EAST-WEST CORRIDOR, FROM ENTRY, LOOKING SOUTH. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

16. MASONRY DETAIL NO. 2, NORTH TRAINING WALL, SHOWING THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. MASONRY DETAIL NO. 2, NORTH TRAINING WALL, SHOWING THE RUBBLE CORE WHERE THE FACING STONES HAVE BEEN REMOVED. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

1. WEST AND SOUTH SIDES, FROM MEDIAN IN B STREET ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. WEST AND SOUTH SIDES, FROM MEDIAN IN B STREET WEST OF 4TH STREET, LOOKING NORTHEAST. - Oakland Naval Supply Center, Firehouse, East of Fourth Street, between A & B Streets, Oakland, Alameda County, CA

Oakland and San Francisco Create Course Pathways through Common Core Mathematics. White Paper

ERIC Educational Resources Information Center

Daro, Phil

2014-01-01

The Common Core State Standards for Mathematics (CCSS-M) set rigorous standards for each of grades 6, 7 and 8. Strategic Education Research Partnership (SERP) has been working with two school districts, Oakland Unified School District and San Francisco Unified School District, to evaluate extant policies and practices and formulate new policies…

1. General view, east end and north side. View to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. General view, east end and north side. View to southwest toward San Francisco-Oakland Bay Bridge, along general alignment of former Interurban Electric Railway main line. Note truncated catenary support arms on power poles; these originally carried overhead power supply catenary line for the electrically-powered interurban cars. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA

322. Dennis Hill, Photographer May 1998 VIEW OF GIRDER SPANS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

322. Dennis Hill, Photographer May 1998 VIEW OF GIRDER SPANS, OAKLAND APPROACH AT TRANSITION TO DOUBLE-DECK ROADWAY, FACING WEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

27. A VIEW TOWARD THE FISHING PIER AT THE EAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. A VIEW TOWARD THE FISHING PIER AT THE EAST END OF THE NORTH TRAINING WALL, SHOWING SIDE WALL CONSTRUCTION. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

14. A CLOSER VIEW OF THE NORTH WALL TOP SURFACE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. A CLOSER VIEW OF THE NORTH WALL TOP SURFACE MASONRY, LOOKING EAST FROM A POINT NEAR THE PREVIOUS VIEW. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

3. WESTERN STORAGE AREA, FROM EAST WALL APPROXIMATELY 50 FEET ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. WESTERN STORAGE AREA, FROM EAST WALL APPROXIMATELY 50 FEET NORTH OF SOUTH WALL, LOOKING WEST. - Oakland Naval Supply Center, Reserve Materials Storehouse, Between I & J Streets, between Fourth & Fifth Streets, Oakland, Alameda County, CA

78 FR 72706 - Notice of Inventory Completion: Michigan State Police, Houghton Lake Post, Houghton Lake, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-03

... remains were collected during road construction in Saginaw County by a private citizen and stored in a... City Road, Houghton Lake, MI 48629, telephone (989) 422-6923, email [email protected] Road, Houghton Lake, MI 48629, telephone (989) 422-6923, email [email protected] , by January 2...

9. GENERAL OBLIQUE VIEW OF SOUTH CORNER OF SHED WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. GENERAL OBLIQUE VIEW OF SOUTH CORNER OF SHED WITH DERRICK AND RAILWAY PASS-TROUGH ON WHARF, LOOKING NORTH - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, IN CONTEXT WITH LOADING YARD AND DERRICK, LOOKING WEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

7. GENERAL VIEW OF SOUTHEAST SIDE OF SHED, SHOWING ALL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. GENERAL VIEW OF SOUTHEAST SIDE OF SHED, SHOWING ALL EIGHTEEN LOADING BAYS, LOOKING WEST FROM ACROSS TURNING BASIN - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

19. GENERAL VIEW, LOOKING SOUTHWEST TO NORTHEAST, SHOWING ENCLOSED OFFICE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. GENERAL VIEW, LOOKING SOUTHWEST TO NORTHEAST, SHOWING ENCLOSED OFFICE UNITS FLANKING OVERHEAD PORT AT NORTHEAST END OF BUILDING - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

356. Delineator Unknown March 1946 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

356. Delineator Unknown March 1946 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; GENERAL DATA; PLAT III - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

16. Photographic copy of photograph (from original 4 x 5 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken prior to June 1942 by unknown photographer. SOUTHWEST BIRDS-EYE VIEW OF WAREHOUSES (BLDGS. 802-805). - Oakland Army Base, Warehouse Type, Tobruk Street, between Warehouse Road & Fifteenth Street, Oakland, Alameda County, CA

75 FR 7536 - Environmental Impact Statement: Ottawa County, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-19

... Baird District Library, 123 Exchange St., Spring Lake. Robinson Township Hall, 12010 120th Ave., Grand... request. FOR FURTHER INFORMATION CONTACT: Ruth Hepfer, Area Engineer at FHWA Michigan Division, 315 W. Allegan Street, Room 201; Lansing, MI 48933; by phone at (517) 702-1847, or email at Ruth[email protected

26. INTERIOR VIEW SHOWING ROW OF TIMBER SUPPORT TOWERS BUILT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. INTERIOR VIEW SHOWING ROW OF TIMBER SUPPORT TOWERS BUILT AS TEMPORARY TRUSS REINFORCEMENT (NOTE STEEL STRUCTURES ATOP TIMBER BRACING) - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

5. SOUTHEAST CORNER AND COURTYARD OPENING, FROM F STREET SIDEWALK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. SOUTHEAST CORNER AND COURTYARD OPENING, FROM F STREET SIDEWALK SOUTHEAST OF BUILDING, LOOKING NORTH-NORTHWEST. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

6. NORTH SIDE, FROM APPROXIMATELY 25 FEET SOUTHEAST OF SOUTHWEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. NORTH SIDE, FROM APPROXIMATELY 25 FEET SOUTHEAST OF SOUTHWEST CORNER OF BUILDING 320, LOOKING SOUTH. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

8. INTERIOR, FIRE ALARM CONTROL ROOM (NORTH OF MAIN GARAGE), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. INTERIOR, FIRE ALARM CONTROL ROOM (NORTH OF MAIN GARAGE), FROM ENTRYWAY, LOOKING NORTH, SHOWING ADDITIONAL 'GAMEWELL' FIRE ALARM SYSTEMS. - Oakland Naval Supply Center, Firehouse, East of Fourth Street, between A & B Streets, Oakland, Alameda County, CA

3. BUILDING 522, EAST SIDE, FROM ACROSS 6TH STREET AT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. BUILDING 522, EAST SIDE, FROM ACROSS 6TH STREET AT ITS INTERSECTION WITH G STREET, LOOKING NORTHWEST. - Oakland Naval Supply Center, Aeronautical Materials Storehouses, Between E & G Streets, between Fourth & Sixth Streets, Oakland, Alameda County, CA

4. BUILDING 313, EAST AND NORTH SIDES, OBLIQUE VIEW, FROM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. BUILDING 313, EAST AND NORTH SIDES, OBLIQUE VIEW, FROM INTERSECTION OF C AND 4TH STREETS, LOOKING SOUTHWEST. - Oakland Naval Supply Center, General Storehouses, Between Third & Fourth Streets, North of A Street, Oakland, Alameda County, CA

33 CFR 165.1192 - Security Zones; Waters surrounding San Francisco International Airport and Oakland International...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zones; Waters... Security Zones; Waters surrounding San Francisco International Airport and Oakland International Airport, San Francisco Bay, California. (a) Locations. The following areas are security zones: (1) San...

33 CFR 165.1192 - Security Zones; Waters surrounding San Francisco International Airport and Oakland International...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zones; Waters... Security Zones; Waters surrounding San Francisco International Airport and Oakland International Airport, San Francisco Bay, California. (a) Locations. The following areas are security zones: (1) San...

33 CFR 165.1192 - Security Zones; Waters surrounding San Francisco International Airport and Oakland International...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zones; Waters... Security Zones; Waters surrounding San Francisco International Airport and Oakland International Airport, San Francisco Bay, California. (a) Locations. The following areas are security zones: (1) San...

33 CFR 165.1192 - Security Zones; Waters surrounding San Francisco International Airport and Oakland International...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zones; Waters... Security Zones; Waters surrounding San Francisco International Airport and Oakland International Airport, San Francisco Bay, California. (a) Locations. The following areas are security zones: (1) San...

33 CFR 165.1192 - Security Zones; Waters surrounding San Francisco International Airport and Oakland International...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zones; Waters... Security Zones; Waters surrounding San Francisco International Airport and Oakland International Airport, San Francisco Bay, California. (a) Locations. The following areas are security zones: (1) San...

10. WIDE GENERAL VIEW OF SHED SHOWING SOUTHWEST FACADE AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. WIDE GENERAL VIEW OF SHED SHOWING SOUTHWEST FACADE AND TRUCK PLATFORM/STAGING AREA AT SOUTHWEST END OF BUILDING, LOOKING NORTHWEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

5. WEST SIDE, FROM ACROSS 3RD STREET, LOOKING EAST, WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. WEST SIDE, FROM ACROSS 3RD STREET, LOOKING EAST, WITH NAVY EXCHANGE RETAIL SERVICE AT TOP OF STAIRS. - Oakland Naval Supply Center, Administration Building-Cafeteria, East of Third Street between D & E Streets, Oakland, Alameda County, CA

29. 4TH STREET FROM NEAR ITS INTERSECTION WITH J STREET, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. 4TH STREET FROM NEAR ITS INTERSECTION WITH J STREET, LOOKING NORTH, WITH WAREHOUSE 333 AT LEFT AND WAREHOUSES 433, 432 & 431 AT RIGHT. - Oakland Naval Supply Center, Maritime Street at Seventh Street, Oakland, Alameda County, CA

22. DETAIL INTERIOR VIEW OF OFFICE, SHOWING ORIGINAL SASH WINDOWS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. DETAIL INTERIOR VIEW OF OFFICE, SHOWING ORIGINAL SASH WINDOWS AND DUCT WORK (OTHER OFFICE INTERIORS ARE GREATLY ALTERED FROM THE ORIGINAL) - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

8. FIRST FLOOR INTERIOR, ONE OF THREE MAIN REFRIGERATIONFREEZER ROOMS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. FIRST FLOOR INTERIOR, ONE OF THREE MAIN REFRIGERATION-FREEZER ROOMS (NO. 4), FROM CENTRAL CORRIDOR OPPOSITE ENTRANCE, LOOKING WEST. - Oakland Naval Supply Center, Cold Storage Warehouse, South of C Street between First & Second Street, Oakland, Alameda County, CA

7. Photographic copy (reduced to 4 x 5 from 8 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photographic copy (reduced to 4 x 5 from 8 X 10 black and white paper reproduction in 1941 appraisal by E.E. Malloy at the Engineering Office, Oakland Army Base, California). Photograph taken between June 1940 and January 1941 by unknown photographer. PARTIAL SOUTH ELEVATION OF VEHICLE MAINTENANCE SHOP (BLDG. 99). - Oakland Army Base, Vehicle Maintenance Shop, Attu Street & Corregidor Avenue, Oakland, Alameda County, CA

6. Photographic copy of photograph (from original 4 x 5 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken April 6, 1942 by unknown photographer. SOUTH AND EAST SIDES OBLIQUE VIEW OF PRIVATE VEHICLE INSPECTION BUILDING (VEHICLE SHED, BLDG. 4). - Oakland Army Base, Private Vehicle Inspection Building, Africa Street & Bataan Avenue, Oakland, Alameda County, CA

11. Photographic copy of photograph (from original 4 x 5 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken January 31, 1942 by unknown photographer. EAST AND NORTH SIDES OBLIQUE VIEW OF EXCHANGE CAFETERIA (BLDG. 60). - Oakland Army Base, Private Exchange Cafeteria, Bataan Avenue & Attu Street, facing Post Headquarters Building, Oakland, Alameda County, CA

21. DETAIL EXTERIOR VIEW OF TWOSTORY OFFICE AT NORTH CORNER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. DETAIL EXTERIOR VIEW OF TWO-STORY OFFICE AT NORTH CORNER OF BUILDING, SHOWING STAIRWAY, SASH WINDOWS, AND TYPICAL COLUMN WITH ROUND CONCRETE BASE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

15. GENERAL VIEW OF NORTHWEST SIDE OF SHED, WITH AN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. GENERAL VIEW OF NORTHWEST SIDE OF SHED, WITH AN OBLIQUE VIEW OF THE STEP-DOWN ROOF AND TWO BANKS OF CLEARSTORY LIGHTS - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ONE-STORY OFFICES, SHOWING TYPICAL COLUMN BASE WITH TIMBER BOLTED TO STEEL 'L' SHOE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

12. BUILDING 324, INTERIOR, ENTRY HALL AND STAIRWAY, FROM SOUTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. BUILDING 324, INTERIOR, ENTRY HALL AND STAIRWAY, FROM SOUTH ENTRY, LOOKING NORTH, WITH HALL LEADING TO GARAGE TO RIGHT OF STAIRWAY. - Oakland Naval Supply Center, Commanding Officers Residences, Between E & F Streets, West of Fourth Street, Oakland, Alameda County, CA

9. INTERIOR, SECOND FLOOR, NORTHSOUTH CORRIDOR IN WEST WING, FROM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. INTERIOR, SECOND FLOOR, NORTH-SOUTH CORRIDOR IN WEST WING, FROM ITS INTERSECTION WITH MAIN EAST-WEST CORRIDOR AND NEAR STAIRWELL, LOOKING NORTH. - Oakland Naval Supply Center, Lodge-Cafeteria, East of Fifth Street, between D & E Streets, Oakland, Alameda County, CA

8. INTERIOR, EMERGENCY ROOM, NORTHEAST OF MAIN CORRIDOR INTERSECTION (NEAR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. INTERIOR, EMERGENCY ROOM, NORTHEAST OF MAIN CORRIDOR INTERSECTION (NEAR WESTERN, MAIN ENTRY), FROM ENTRY IN NORTHWESTERN CORNER, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

3. SOUTH SIDE, OBLIQUE VIEW, FROM F STREET SIDEWALK, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. SOUTH SIDE, OBLIQUE VIEW, FROM F STREET SIDEWALK, LOOKING NORTHWEST, WITH BUILDING 222 ACROSS 3RD STREET AT LEFT. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

7. INTERIOR, KITCHEN/GALLEY, FROM NEAR SOUTHEAST CORNER OF ROOM, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. INTERIOR, KITCHEN/GALLEY, FROM NEAR SOUTHEAST CORNER OF ROOM, LOOKING WEST, WITH PASSAGES TO REFRIGERATION AND FOOD PREPARATION ROOMS AT CENTER. - Oakland Naval Supply Center, Lodge-Cafeteria, East of Fifth Street, between D & E Streets, Oakland, Alameda County, CA

25. PARK AND ROSE GARDEN ALONG SOUTH SIDE OF F ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. PARK AND ROSE GARDEN ALONG SOUTH SIDE OF F STREET, FROM HALFWAY BETWEEN 3RD AND 4TH STREETS, LOOKING WEST. COMMANDING OFFICER'S RESIDENCES AT FAR RIGHT. - Oakland Naval Supply Center, Maritime Street at Seventh Street, Oakland, Alameda County, CA

Results of bulk sediment analysis and bioassay testing on selected sediments from Oakland Inner Harbor and Alcatraz disposal site, San Francisco, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Word, J Q; Ward, J A; Woodruff, D L

1990-09-01

The Battelle/Marine Sciences Laboratory (MSL) was contracted by the US Army Corps of Engineers, San Francisco District, to perform bulk sediment analysis and oyster larvae bioassays (elutriate) on sediments from Inner Oakland Harbor, California. Analysis of sediment characteristics by MSL indicated elevated priority pollutants, PAHs, pesticides, metals, organotins, and oil and grease concentrations, when compared to Alcatraz Island Dredged Material Disposal Site sediment concentrations. Larvae of the Pacific oyster, Crassostrea gigas, were exposed to seawater collected from the Alcatraz Island Site water, and a series of controls using water and sediments collected from Sequim Bay, Washington. Exposure of larvae tomore » the Alcatraz seawater and the 50% and 100% elutriate concentrations from each Oakland sediment resulted in low survival and a high proportion of abnormal larvae compared to Sequim Bay control exposures. MSL identified that field sample collection, preservation, and storage protocols used by Port of Oakland contractors were inconsistent with standard accepted practices. 23 refs., 10 figs., 40 tabs.« less

72. MISSISSIPPI, MONROE CO. MAP OF MONROE COUNTY, ca. 1925 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

72. MISSISSIPPI, MONROE CO. MAP OF MONROE COUNTY, ca. 1925 Broad side of map of Monroe Co., 'Compliments of Home Mortgage & Realty Co., Amory, Miss.' Orig. scale: ca. 1 in. to 2 mi. No date. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

5. OBLIQUE VIEW OF SOUTH AND EAST FACES. THE REFRIGERATION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. OBLIQUE VIEW OF SOUTH AND EAST FACES. THE REFRIGERATION SPACE PROJECTING FROM THE SOUTH SIDE OF THE BUILDING WAS ADDED IN MARCH 1944. - Oakland Army Base, Private Exchange Cafeteria, Bataan Avenue & Attu Street, facing Post Headquarters Building, Oakland, Alameda County, CA

2. BUILDING 413, WEST SIDE AND OBLIQUE VIEW OF NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. BUILDING 413, WEST SIDE AND OBLIQUE VIEW OF NORTH SIDE, FROM INTERSECTION OF C AND 4TH STREETS, LOOKING SOUTHEAST. - Oakland Naval Supply Center, Heavy Materials & Paint-Oil Storehouses, Between Fourth & Sixth streets, between B & D Streets, Oakland, Alameda County, CA

13. THE SAME NORTH TRAINING WALL TOP SURFACE, LOOKING EAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. THE SAME NORTH TRAINING WALL TOP SURFACE, LOOKING EAST FROM ATOP ADJACENT RIPRAP. THE TRAINING WALL IS TO THE RIGHT OF THE JUMBLED, LIGHT TONED RIPRAP. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

8. BUILDING 332, INTERIOR, HALLWAY NEAR MAIN ENTRANCE IN NORTHWESTERN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. BUILDING 332, INTERIOR, HALLWAY NEAR MAIN ENTRANCE IN NORTHWESTERN PORTION OF BUILDING, LOOKING NORTH, WITH LOBBY TO LEFT AND CONFERENCE ROOM TO RIGHT. - Oakland Naval Supply Center, Dry Provisions Storehouses, Between Third & Fourth Streets, Between G & L Street, Oakland, Alameda County, CA

396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS I; DRG. NO. 68 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; ERECTION; DRG. NO. 43 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

393. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

393. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER-E3; GENERAL DETAILS; DRG. NO. 47 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

369. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

369. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; CAISSON ANCHORAGE; DRG. NO. 22 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS II; DRG. NO. 69 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

398. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

398. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; GENERAL PLAN; TOWER E-9; DRG. NO. 59 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

30. VERTICAL AERIAL VIEW OF THE MOUTH OF THE FEDERAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. VERTICAL AERIAL VIEW OF THE MOUTH OF THE FEDERAL CHANNEL, SCALE 1:14,400. TO THE SOUTH OF THE CHANNEL ARE THE RUNWAYS OF THE FORMER ALAMEDA NAVAL AIR STATION; TO THE NORTH ARE THE BERTHS AND BUILDINGS OF THE FORMER NAVAL SUPPLY CENTER, OAKLAND. Date and time of photography '12-9-98 10:51." - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

Oakland Unified School District Community Schools: Understanding Implementation Efforts to Support Students, Teachers, and Families

ERIC Educational Resources Information Center

Fehrer, Kendra; Leos-Urbel, Jacob

2015-01-01

In 2010, Oakland Unified School District (OUSD) launched an initiative to transform all district schools into full service community schools. The community school design provides integrated supports to students and fosters a school climate conducive to academic, social, and emotional learning. Interventions span in-school and out-of-school time,…

4. FIRST FLOOR INTERIOR, AMMONIA COMPRESSION DYNAMOS IN MACHINERY ROOM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. FIRST FLOOR INTERIOR, AMMONIA COMPRESSION DYNAMOS IN MACHINERY ROOM ALONG SOUTH SIDE OF WESTERN PORTION OF BUILDING, FROM EASTERN ENTRANCE TO MACHINERY ROOM, LOOKING WEST. - Oakland Naval Supply Center, Cold Storage Warehouse, South of C Street between First & Second Street, Oakland, Alameda County, CA

4. DETAIL VIEW OF EAST CORNER, SHOWING RECENT ADDITION OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. DETAIL VIEW OF EAST CORNER, SHOWING RECENT ADDITION OF WINDOWS TO SOUTHEAST SIDE AND RECENT CLADDING IN CONTRAST TO ORIGINAL SHIPLAP SIDING INTACT ON NORTHEAST SIDE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

24. DETAIL VIEW OF COLUMN #072 DEVIATING FROM VERTICAL IN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. DETAIL VIEW OF COLUMN #072 DEVIATING FROM VERTICAL IN ROW OF INTACT COLUMNS, LOOKING NORTHEAST TO SOUTHWEST. (NOTE BOLTED BLOCK SCABBED TO COLUMN AS JOIST/TRUSS SUPPORT) - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

23. DETAIL VIEW OF COLUMN #072, SHOWING TOP LEANING TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. DETAIL VIEW OF COLUMN #072, SHOWING TOP LEANING TO SOUTHEAST (LEFT IN PHOTOGRAPH) WITH STEEL PIPE AS TEMPORARY SUPPORT (SEE PHOTOGRAPH NO. CA-125-A-24 BELOW) - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

11. BUILDING 332, INTERIOR, WESTERN STOREROOM, FROM FIRE DOOR IN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. BUILDING 332, INTERIOR, WESTERN STOREROOM, FROM FIRE DOOR IN EAST WALL, LOOKING WEST, WITH ENCLOSED OFFICE AREA (PHOTOS AA-7 &-8) BEYOND WALL ON RIGHT. - Oakland Naval Supply Center, Dry Provisions Storehouses, Between Third & Fourth Streets, Between G & L Street, Oakland, Alameda County, CA

8. BUILDING 313, INTERIOR, EAST WALL OF EASTERN STOREROOM, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. BUILDING 313, INTERIOR, EAST WALL OF EASTERN STOREROOM, LOOKING NORTHEAST, WITH TIME CLOCK AND FIRE SPRINKLER CONTROLS ON WALL, AND ENTRY TO OFFICE AREA AT LEFT. - Oakland Naval Supply Center, General Storehouses, Between Third & Fourth Streets, North of A Street, Oakland, Alameda County, CA

29. DETAIL, RUINS OF THE NORTH TRAINING WALL AT THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. DETAIL, RUINS OF THE NORTH TRAINING WALL AT THE EAST END, WHERE IT TURNS TO THE NORTH AND IS BURIED. LOOKING WEST FROM THE MIDDLE HARBOR PARK FISHING PIER. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

25. NORTH TRAINING WALL, EAST SECTION, SIDE WALL CONSTRUCTION, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. NORTH TRAINING WALL, EAST SECTION, SIDE WALL CONSTRUCTION, LOOKING WEST FROM A POINT ABOUT 500 FEET FROM THE MIDDLE HARBOR PARK FISHING PIER. (Panoramic view 1 of 2). - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

Where Will Urban High School Teachers for the 21st Century Come From?

ERIC Educational Resources Information Center

Follo, Eric; Hoerr, Bill; Vorheis-Sargent, Ann

2002-01-01

Describes urban teacher supply problem in Oakland County, Michigan. Reviews literature on urban teacher supply problem nationally. Describes Michigan's alternative teacher-certification program and Oakland University's partnership with the Pontiac School District to prepare teachers for teaching in urban schools. (Contains 50 references.) (PKP)

9. INTERIOR, BUILDING 320, MAIN GALLEY, FROM SOUTH WALL OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. INTERIOR, BUILDING 320, MAIN GALLEY, FROM SOUTH WALL OF ROOM, LOOKING NORTH, WITH KITCHEN HOOD OVERHEAD, AND DOOR TO REFRIGERATION ROOM ON OPPOSITE WALL. - Oakland Naval Supply Center, Administration Building-Cafeteria, East of Third Street between D & E Streets, Oakland, Alameda County, CA

11. BUILDING 421, WEST SIDE (OBLIQUE VIEW) AND WESTERN PORTION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. BUILDING 421, WEST SIDE (OBLIQUE VIEW) AND WESTERN PORTION OF SOUTHERN SIDE, FROM SOUTHWESTERN CORNER OF INTERSECTION OF E AND 4TH STREETS, LOOKING NORTHEAST. - Oakland Naval Supply Center, General Storehouses, Between Third & Fourth Streets, North of A Street, Oakland, Alameda County, CA

359. Delineator Unknown April 1935 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

359. Delineator Unknown April 1935 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; BRIDGE RAILWAY SAN FRANCISCO LOOP; DETAILS OF VIADUCT; FINAL REPORT; DRG. NO. 92 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

403. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

403. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; SAN FRANCISCO - OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 2 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; TYPICAL DETAILS; DRG. NO. 42 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

366. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

366. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO CABLE BENT; DRG. NO. 33 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

382. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

382. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; GENERAL ELEVATIONS & SECTIONS; DRG. NO. 28 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

383. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

383. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; TYPICAL BASE DETAILS; DRG. NO. 29 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

399. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

399. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; 504FOOT SPANS; TYPICAL DETAILS; DRG. NO. 64 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

360. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

360. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; TYPICAL SECTIONS; DRG. NO. 13 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

391. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

391. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER E1; GENERAL DETAILS; DRG. NO. 45 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

395. J.L.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

395. J.L.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; TOWER E-4; GENERAL PLAN; DRG. NO. 58 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

361. W.J.M., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

361. W.J.M., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; TYPICAL SECTIONS; DRG. NO. 14 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

386. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

386. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; TYPICAL TOP DETAILS; DRG. NO. 31 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Providing Options for At-Risk Youth: The Health and Media Academies in Oakland. Final Report.

ERIC Educational Resources Information Center

Guthrie, Larry F.; And Others

The Health Academy and the Media Academy, two innovative high school intervention programs for at-risk youth in Oakland (California), are examined. A collaborative effort of the school district, business, and community, the academies are school-within-a-school programs that engage about 120 at-risk students each in specific academic curricula for…

7. Photographic copy of photograph (from original 4 x 5 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken January 28, 1942 by unknown photographer. AT CENTER RIGHT, SOUTH AND EAST SIDES OBLIQUE VIEW OF POST HEADQUARTERS BUILDING (ADMINISTRATION BUILDING, BLDG. 1) TAKEN FROM EAST SIDE OF MARITIME STREET. - Oakland Army Base, Maritime Street at West Grand Avenue, Oakland, Alameda County, CA

402. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

402. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; SAN FRANCISCO - OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 1A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

380. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

380. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CABLES & ATTACHMENTS WEST BAY CROSSING; CABLE BANDS; CONTRACT NO. 6A; DRAWING NO. 3 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

385. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

385. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; ROCKER POSTS AND BEARING; CONTRACT NO. 6; DRAWINGS NO. 42 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

384. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

384. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; BRACING AND ROCKER BRACKET DETAILS; DRG. NO. 30 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

379. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

379. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CABLES AND ATTACHMENTS - WEST BAY CROSSING; SPLAY CASTINGS; CONTRACT NO. 6A; DRAWING NO. 4 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

373. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

373. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; PIER W-4; DETAILS OF A-FRAME; DRG. NO. 19 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

370. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

370. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; PIER W-4; PLANS AND ELEVATIONS; DRG. NO. 17 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

400. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

400. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER E-6 TO E-23; TYPICAL DETAILS; DRG. NO. 52 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Determining Spatial Variability in PM2.5 Source Impacts across Detroit, MI

EPA Science Inventory

Intra-urban variability in air pollution source impacts was investigated using receptor modeling of daily speciated PM_2.5 measurements collected at residential outdoor locations across Detroit, MI (Wayne County) as part of the Detroit Exposure and Aerosol Research Stud...

Safety evaluation of the SCATS control system, final report.

DOT National Transportation Integrated Search

2010-09-01

Since 1992, traffic signals in Oakland County and a portion of Macomb and Wayne Counties of Michigan have been : converted to the Sydney Coordinated Adaptive Traffic System (SCATS). County traffic engineers have been : adjusting various SCATS paramet...

Southeast Michigan Snow and Ice Management (SEMSIM)

DOT National Transportation Integrated Search

2001-07-01

The Southeast Michigan Snow and Ice Management (SEMSIM) partnership includes the Detroit Department of Public Works, the Road Commission of Macomb County, the Road Commission for Oakland County, and the Wayne County Department of Public Services. The...

Addressing Structural Barriers to HIV Care among Triply Diagnosed Adults: Project Bridge Oakland.

PubMed

Powers, Christina; Comfort, Megan; Lopez, Andrea M; Kral, Alex H; Murdoch, Owen; Lorvick, Jennifer

2017-05-01

People who are "triply diagnosed" with HIV, mental health issues, and substance-related disorders face tremendous barriers connecting to and remaining in HIV care. Authors of this article implemented Project Bridge Oakland (PBO), an intervention based on harm reduction and trauma-informed care, to help maintain continuity of care for triply diagnosed adults through cycles of criminal justice involvement. From August 2011 to December 2014, a clinical social worker and an HIV physician provided intensive case management for 19 clients living in Oakland, California. By working with clients across a multitude of community, clinic, and correctional spaces while maintaining a low threshold for services, the social worker was able to engage a severely marginalized population in HIV care. This article details the PBO strategies for assisting with a wide range of services needed for community stabilization, navigating criminal justice involvement, and establishing a therapeutic relationship through mundane practices such as eating and waiting for appointments. This article illustrates how programs aimed at stabilizing triply diagnosed clients in the community and connecting them to HIV care require coordination among providers, outreach to engage clients, ample time to work with them, and flexibility to account for the complexities of their day-to-day lives and experiences. © 2017 National Association of Social Workers.

Ox Mountain Sanitary Landfill Apanolio Canyon Expansion Site, San Mateo County, California. Volume 2. Appendix

DTIC Science & Technology

1989-04-01

old-growth forest located between Sonoma County and the Oregon border. The exact northern limit of the small southern I population is not known...meadow habitat on the inland side of sand dunes at Pt. Reyes (Matin County) and Bodega Bay ( Sonoma County ). Historically, the silverspot also probably...and Sonoma County (6.5 mi. northeast of Penngrove). Collection dates ranged from 27IJanuary to 30 July. Most of the species of Hydrochara are similar

Mobile Motion Capture--MiMiC.

PubMed

Harbert, Simeon D; Jaiswal, Tushar; Harley, Linda R; Vaughn, Tyler W; Baranak, Andrew S

2013-01-01

The low cost, simple, robust, mobile, and easy to use Mobile Motion Capture (MiMiC) system is presented and the constraints which guided the design of MiMiC are discussed. The MiMiC Android application allows motion data to be captured from kinematic modules such as Shimmer 2r sensors over Bluetooth. MiMiC is cost effective and can be used for an entire day in a person's daily routine without being intrusive. MiMiC is a flexible motion capture system which can be used for many applications including fall detection, detection of fatigue in industry workers, and analysis of individuals' work patterns in various environments.

15. BUILDING 324 INTERIOR, MASTER BEDROOM, FROM SOUTHEASTERN CORNER OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. BUILDING 324 INTERIOR, MASTER BEDROOM, FROM SOUTH-EASTERN CORNER OF ROOM, LOOKING NORTHWEST, WITH ENTRY FROM HALLWAY, ENTRY TO BATH AND CLOTHES CLOSET IN SEQUENCE FROM LEFT TO RIGHT. - Oakland Naval Supply Center, Commanding Officers Residences, Between E & F Streets, West of Fourth Street, Oakland, Alameda County, CA

32. OBLIQUE AERIAL VIEW TO THE SOUTHWEST, SHOWING THE FEDERAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. OBLIQUE AERIAL VIEW TO THE SOUTHWEST, SHOWING THE FEDERAL CHANNEL IN RELATION TO SAN FRANCISCO BAY AND SAN BRUNO MOUNTAIN AT TOP CENTER. Date and time of photography "12-9-98 10:58." - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

2. GRADIENT INTO A STREET IN SUPPLY CENTER, FROM SIDEWALK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. GRADIENT INTO A STREET IN SUPPLY CENTER, FROM SIDEWALK ON SOUTHERN SIDE OF BRIDGE AT A POINT EAST OF 4TH STREET, LOOKING WEST, WITH MONUMENT AT END OF A STREET. - Oakland Naval Supply Center, Maritime Street Overpass, Connecting Seventh Street & Third Street, Oakland, Alameda County, CA

404. Delineator Unknown June 1, 1933 STUDY FOR TOP OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

404. Delineator Unknown June 1, 1933 STUDY FOR TOP OF SUSPENSION TOWERS; SAN FRANCISCO OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 7-A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

377. F.A.N. and Q.E.D., Delineators Date Unknown STATE OF CALIFORNIA; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

377. F.A.N. and Q.E.D., Delineators Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; YERBA BUENA CABLE BENT; DRG. NO. 34 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

7. INTERIOR, MAIN GARAGE, SOUTHERN WALL, FROM CLOSE TO WALL, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. INTERIOR, MAIN GARAGE, SOUTHERN WALL, FROM CLOSE TO WALL, LOOKING SOUTH, SHOWING 'GAMEWELL' FIRE ALARM TAPE CONTROL SYSTEM (TECHNOLOGY CIRCA 1910) AT CENTER, AND ENTRY TO OFFICE AT FAR RIGHT. - Oakland Naval Supply Center, Firehouse, East of Fourth Street, between A & B Streets, Oakland, Alameda County, CA

Diagnostic and prognostic potential of serum miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a and miR-429 in ovarian cancer patients.

PubMed

Meng, Xiaodan; Joosse, Simon A; Müller, Volkmar; Trillsch, Fabian; Milde-Langosch, Karin; Mahner, Sven; Geffken, Maria; Pantel, Klaus; Schwarzenbach, Heidi

2015-11-03

Owing to late diagnosis in advanced disease stages, prognosis of patients with epithelial ovarian cancer (EOC) is poor. The quantification of deregulated levels of microRNAs could facilitate earlier diagnosis and improve prognosis of EOC. Seven microRNAs (miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a and miR-429) were quantified in the serum of 180 EOC patients and 66 healthy women by TaqMan PCR microRNA assays. Median follow-up time was 21 months. The effects of miR-7 and miR-429 on apoptosis, cell proliferation, migration and invasion were investigated in two (EOC) cell lines. Serum levels of miR-25 (P=0.0001) and miR-93 (P=0.0001) were downregulated, whereas those of miR-7 (P=0.001) and miR-429 (P=0.0001) were upregulated in EOC patients compared with healthy women. The four microRNAs discriminated EOC patients from healthy women with a sensitivity of 93% and a specificity of 92%. The levels of miR-429 positively correlated with CA125 values (P=0.0001) and differed between FIGO I-II and III-IV stages (P=0.001). MiR-429 was an independent predictor of overall survival (P=0.011). Overexpressed miR-429 in SKOV3 cells led to suppression of cell migration (P=0.037) and invasion (P=0.011). Increased levels of miR-7 were associated with lymph node metastases (P=0.0001) and FIGO stages III-IV (P=0.0001). Overexpressed miR-7 in SKOV3 cells resulted in increased cell migration (P=0.001) and invasion (P=0.011). Additionally, the increased levels of miR-376a correlated with FIGO stages III-IV (P=0.02). Our data indicate the diagnostic potential of miR-7, miR-25, miR-93 and miR-429 in EOC and the prognostic potential of miR-429. This microRNA panel may be promising molecules to be targeted in the treatment of EOC.

8. Photographic copy of photograph (from original 4 x 5 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken May 13, 1942 by unknown photographer. BIRDS-EYE VIEW FROM THE SOUTH OF PRINTING PLANT (STOREHOUSE, BLDG. 85) AT CENTER TOP; STOREHOUSE (BLDG. 88) AT TOP RIGHT; GENERAL PURPOSE ADMINISTRATION BUILDING (BLDG. 90) AT CENTER. - Oakland Army Base, Maritime Street at West Grand Avenue, Oakland, Alameda County, CA

Southeast Michigan Snow and Ice Management (SEMSIM) : final evaluation at end of winter season, year 2004

DOT National Transportation Integrated Search

2004-08-01

In the early spring of 1999, the Detroit Department of Public Works, the Road Commission of Macomb County, the Road Commission for Oakland County, and the Wayne County Department of Public Services formed the Southeast Michigan Snow and Ice Managemen...

392. J.R.L., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

392. J.R.L., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - EAST BAY CROSSING; PIER El; ANCHORAGE STEEL WORK; CONTRACT NO. 7; SUP. DRAWING NO. 72A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; SUSPENDED STRUCTURE; SIDE SPAN TRUSSES AT ANCHORAGES; CONTRACT NO. 6; DRAWING NO. 40 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Becoming a Community School: A Study of Oakland Unified School District Community School Implementation, 2015-2016

ERIC Educational Resources Information Center

Fehrer, Kendra; Leos-Urbel, Jacob; Messner, Erica; Riley, Nicole

2016-01-01

Since 2014, Oakland Unified School District (OUSD) has partnered with the Gardner Center for Youth and Their Communities at Stanford University (Gardner Center) to support OUSD's efforts to assess, enhance, and scale their community schools work. They began by working with the district to develop a System Strategy Map to articulate the district's…

10. BUILDING 332, LARGE STOREROOM IN WESTERN PORTION OF BUILDING, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. BUILDING 332, LARGE STOREROOM IN WESTERN PORTION OF BUILDING, FROM APPROXIMATELY 60 FEET EAST OF WEST WALL AND GARAGE DOOR IN PHOTOGRAPH AA-9, LOOKING EAST, WITH ADJOINING STOREROOMS VISIBLE THROUGH FIRE DOORS. - Oakland Naval Supply Center, Dry Provisions Storehouses, Between Third & Fourth Streets, Between G & L Street, Oakland, Alameda County, CA

401. J.W.P.C., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

401. J.W.P.C., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIERS E6 TO E-22; SEQUENCE OF OPERATION; DETAILS OF EQUIPMENT; DRG. NO. 53 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

387. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

387. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; TOWERS 2, 3, 5 & 6; BRACING DETAILS - LOWER DECK; CONTRACT NO. 6; DRAWING NO. 27 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

367. J.W.G., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

367. J.W.G., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; PIER NO. 5; GENERAL PLAN & ELEVATION; CONTRACT NO. 2; SUP. DRAWING NO. 10A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

390. Delineator Unknown October 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

390. Delineator Unknown October 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - EAST BAY CROSSING; SPANS YBI TO El - E9 TO El ; TYPICAL CROSSSECTION; CONTRACT NO. 7; SUP. DRAWING NO. 82 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Up-Regulation of miR-21, miR-25, miR-93, and miR-106b in Gastric Cancer

PubMed

LArki, Pegah; Ahadi, Alireza; Zare, Ali; Tarighi, Shahriar; Zaheri, Mahrokh; Souri, Mojgan; Zali, Mohammad Reza; Ghaedi, Hamid; Omrani, Mir Davood

2018-06-03

Differential expression profile of microRNAs (miRNAs) could be a diagnosis signature for the monitoring of gastric cancer (GC) progression. In this study, we focus on the comparison of expression levels of miR-21, miR-25, miR-93, miR-106b, and miR-375 during the sequential pattern of GC development, including normal gastric, gastric dysplasia, and GC sample. We used SYBR Green-based quantitative-PCR to quantify miRNAs expression. Our analysis revealed the increased expression levels of miR-21 (p = 0.034), miR-25 (p = 0.0003) miR-93 (p = 0.0406), and miR-106b (p = 0.023) in GC samples. In addition, GC patients with positive lymph node metastasis showed the up-regulation of miR-25, miR-93, and miR-106b (p < 0.05). Our findings suggested that miR-21, miR-25, miR-93, and miR-106b altered expression in GC, and some of them may be further investigated as biomarkers for GC early detection and prognosis prediction.

Lead Concentration Levels in Water Samples Collected in Alameda County, CA

NASA Astrophysics Data System (ADS)

Sethy, D.; Hoang, R.; Yu, I.; Hernandez, N.; Fang, K.; Zhang, W.; Li, J.; Munui, K. N.; Sot, R.; Luong, K.; Bonzo, R.; Sankar, R.; Chiu, D.; Rodriguez, V. A.

2016-12-01

The recent health crisis in Flint, Michigan has attracted an amount of interest in other public utilities' water supplies and infrastructure with regards to concerns over the presence of lead. In an effort to begin assessing the potential for a health crisis similar to that experienced in Flynt, during 2016 our team measured lead concentration levels in water samples by collected in Alameda County. More than 12 sites were selected from which samples were collected. These sites included parks, schools, and private residences. At each site 500mL samples were collected and prepared for later analysis. Samples were subjected to an analytical chemistry technique designed to isolate and concentrate lead to detectable levels of 1 part per billion (ppb). All 8 samples yielded detectable levels of lead; all samples were also well below the EPA regulatory 15 ppb. Two samples collected in West Oakland parks were found to have the highest and lowest levels: DeFremery (4 ppb) and Raimondi (1ppb), respectively. Though preliminary in nature, results from this study suggest that further investigations should be undertaken to assess possible lead contamination associated with drinking water sources in Alameda County.

19. BLUEPRINT, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. BLUEPRINT, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of Ms. 50 Map of Tombigbee River at Waverly, 'Proposed Crossing.' 12 May 1888. Credt: Columbus & Greenville, RR, Columbus, Ms. DWG S-3-343. Sarcone Photography, ColumbuS, Ms. Sept 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

20. BLUEPRINT, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY L.% MI. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. BLUEPRINT, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY L.% MI. S of MS. 50 Proposed bridge, by Phoenis Bridge Co., Phoenixville, Pa. 218-foot turn span, with load bearing. 21 May 1914. Act. size: 16X35 in. Credit: Columbus and Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sept 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

Economic Impact Study, 1992.

ERIC Educational Resources Information Center

Oakland Community Coll., Farmington, MI. Office of Institutional Planning and Analysis.

In an effort to determine the economic benefits that Oakland Community College (OCC) in Michigan had on the local economy of Oakland County and on the state during fiscal year 1991-92, an economic impact study was conducted. The study utilized an economic impact model consisting of three major components: direct institutional expenditures; direct…

394. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

394. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIERS E-2, E-3, E-4, E-5; PLANT LAYOUT AND CAISSON ANCHORAGES; DRG. NO.51 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

414. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

414. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; TYPICAL SECTIONS; SHEET NO. 5; DRAWING NO. C-4028-5R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

371. A.J.M. and D.L.S., Delineators April 1934. STATE OF CALIFORNIA; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

371. A.J.M. and D.L.S., Delineators April 1934. STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; PIER NO. 4; VERTICAL SECTIONS; CONTRACT NO. 2; SUP. DRAWING NO. 17A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

77 FR 36041 - Nevada 5, Inc. and Oakland Transportation Holdings LLC-Control Exemption-GTR Leasing LLC and US...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35635] Nevada 5, Inc. and Oakland Transportation Holdings LLC--Control Exemption--GTR Leasing LLC and US Rail Holdings LLC \\1\\ \\1... date of the exemption (30 days after the verified notice was filed). Applicants represent that: (1) The...

13. RAILROAD BRIDGE MISSISSIPPI, MONROE CO., ABERDEEN 1.5 mi. NW ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. RAILROAD BRIDGE MISSISSIPPI, MONROE CO., ABERDEEN 1.5 mi. NW of Amory. St. Louis and San Francisco RR bridge. Steam locomotive and coal train cross bridge on 10 August 1921. Credit: Owned by Jack Donnell, Columbus, Ms., photographer. Copied by Sarcone Photography, Columbus, Ms. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

Relationship of Shallow Groundwater Quality to Hydraulic Fracturing Activities in Antrim and Kalkaska Counties, MI

NASA Astrophysics Data System (ADS)

Stefansky, J. N.; Robertson, W. M.; Chappaz, A.; Babos, H.; Israel, S.; Groskreutz, L. M.

2015-12-01

Hydraulic fracturing (fracking) of oil and natural gas (O&G) wells is a widely applied technology that can increase yields from tight geologic formations. However, it is unclear how fracking may impact shallow groundwater; previous research into its effects has produced conflicting results. Much of the worry over potential impacts to water quality arises from concerns about the produced water. The water produced from O&G formations is often salty, contains toxic dissolved elements, and can be radioactive. If fracking activities cause or increase connectivity between O&G formations and overlying groundwater, there may be risks to aquifers. As one part of a groundwater quality study in Antrim and Kalkaska Counties, MI, samples were collected from the unconfined glacial aquifer (3-300 m thick) and produced water from the underlying Antrim formation, a shallow (180-670 m deep) natural gas producing black shale. Groundwater samples were collected between 200 to 10,000 m distance from producing Antrim gas wells and from a range of screened intervals (15-95 m). Samples were analyzed for major constituents (e.g., Br, Cl), pH, conductivity, and dissolved oxygen (DO). The specific conductance of groundwater samples ranged from 230-1020 μS/cm; DO ranged from 0.4-100% saturation. Preliminary results show a slight inverse correlation between specific conductance and proximity to producing Antrim wells. The observed range of DO saturation in glacial aquifer groundwater appears to be related to both screened depth of the water wells and proximity to Antrim wells. During sampling, some well owners expressed concerns about the effects of fracking on groundwater quality and reported odd smells and tastes in their water after O&G drilling occurred near their homes. The results of this study and reported observations provide evidence to suggest a potential hydrogeological connection between the Antrim formation and the overlying glacial aquifer in some locations; it also raises

A model of systems integration to facilitate ITS deployment : FAST-TRAC phase IV deliverable

DOT National Transportation Integrated Search

2003-04-01

The FAST-TRAC (Faster and Safer Travel through Traffic Routing and Advanced Controls) Intelligent Transportation Systems (ITS) Field Test in Oakland County, an urbanized county in metropolitan Detroit, represents a large and successful systems integr...

7. FLOOR PLAN, PLOT PLAN, ELEVATIONS, SHEET NO. 11044/1 OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. FLOOR PLAN, PLOT PLAN, ELEVATIONS, SHEET NO. 1-10-44/1 OF 11. (NOTE: BUILDINGS 821-823 WERE CONSTRUCTED FROM A SINGLE SET OF PLANS. SOME DRAWINGS ARE TYPICAL OF ALL 3 STRUCTURES, CERTAIN OTHER DRAWINGS IN THE SAME SET ARE BUILDING-SPECIFIC.) - Oakland Army Base, Storehouse Type, Ukraine & Maritime Streets, Oakland, Alameda County, CA

413. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

413. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; CONTINUOUS SPANS - LONGITUDINAL GIRDERS; SHEET NO. 18; DRAWING NO. C-4028-18R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

363. A.C.S., Delineator March 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

363. A.C.S., Delineator March 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6A; SUPERSTRUCTURE - WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G4866; SHEET NO E3 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

9. Detail, original door in south leanto. The current project ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Detail, original door in south lean-to. The current project will modify this opening to allow handicap access. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA

30. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Detail: Turn machinery. DWG S-3-325. Contr. #7236. Lower right quarter of sheet: Lateral elevation of turn table. July 1914. Credit: Columbus and Greenville RR, Columbus, MS. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

31. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Detail: Turn machinery. DWG S-3-325. Contr. #7236. Upper left quarter of sheet: plan and longitudinal elevation of Wedges. July 1914. Credit: Columbus and Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sep 1978 - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

29. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5. mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5. mi. S of MS. 50 Detail: Turn machinery. DWG S-3-325. Contr. #7236. Lower right quarter of sheet: Lateral elevation of turn table. July 1914. Credit: Columbus and Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

22. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Proposed 218-foot turn span, submitted by the Wisconsin Bridge & Iron Co., Milwaukee, Wisconsin 8 May 1914. Act size: approx. 23x34 in. Credit: Columbus & Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, MS. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

21. RAILROAD, RR. BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. RAILROAD, RR. BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of Ms. 50 Proposed 218-foot turn span, design #2339 of Virginia Bridge and Iron Co., Roanoke, Va. 13 May 1914. Act. size: 16x11 in. Credit: Columbus & Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

27. RAILROAD, RR. BRIDGE MISSISSIPPI, CLAY CO. WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. RAILROAD, RR. BRIDGE MISSISSIPPI, CLAY CO. WAVERLY 1.5 mi. S of MS. 50 Stress Sheet of 1888 Bridge: Conroy & Sinks, Consulting Engineers, Chicago. July 21, 1910. DWG #978. Act. size around border: 18x11 in. Credit: Columbus and Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

75 FR 62571 - Labor Surplus Area Classification Under Executive Orders 12073 and 10582

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-12

... City, MA Michigan Alcona County, MI Alcona County, MI Alger County, MI Alger County, MI Alpena County, MI Alpena County, MI Antrim County, MI Antrim County, MI Arenac County, MI Arenac County, MI Balance...

A Child's Birthright: To Live in a Family. A Vicarious Visit to Macomb-Oakland Regional Center, Mt. Clemens, Michigan.

ERIC Educational Resources Information Center

Rosenau, Nancy, Ed.

This paper recounts the Macomb-Oakland (Michigan) Regional Center's 17-year successful experience in eliminating institutional care for children with developmental disabilities (many of them with severe disabilities) and providing all such children with specialized foster home placements. A paper by the Center's director, Gerald Provencal,…

364. J.G.M., Delineator February 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

364. J.G.M., Delineator February 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6; SUPERSTRUCTURE - WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE CABLE BENT CASTING; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4852 C; SHEET NO. 100 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

378. A.C.S., Delineator March 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

378. A.C.S., Delineator March 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6A; SUPERSTRUCTURE - WEST BAY CROSSING; YERBA BUENA ANCHORAGE & CABLE BENT. AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4866; SHEET NO. E4 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

415. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

415. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; Y.B. ANCHORAGE - FLOOR BEAMS YA-5 AND BENT 3; SHEET NO. 22; DRAWING NO. C-4028-22R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Case-control geographic clustering for residential histories accounting for risk factors and covariates.

PubMed

Jacquez, Geoffrey M; Meliker, Jaymie R; Avruskin, Gillian A; Goovaerts, Pierre; Kaufmann, Andy; Wilson, Mark L; Nriagu, Jerome

2006-08-03

Methods for analyzing space-time variation in risk in case-control studies typically ignore residential mobility. We develop an approach for analyzing case-control data for mobile individuals and apply it to study bladder cancer in 11 counties in southeastern Michigan. At this time data collection is incomplete and no inferences should be drawn - we analyze these data to demonstrate the novel methods. Global, local and focused clustering of residential histories for 219 cases and 437 controls is quantified using time-dependent nearest neighbor relationships. Business address histories for 268 industries that release known or suspected bladder cancer carcinogens are analyzed. A logistic model accounting for smoking, gender, age, race and education specifies the probability of being a case, and is incorporated into the cluster randomization procedures. Sensitivity of clustering to definition of the proximity metric is assessed for 1 to 75 k nearest neighbors. Global clustering is partly explained by the covariates but remains statistically significant at 12 of the 14 levels of k considered. After accounting for the covariates 26 Local clusters are found in Lapeer, Ingham, Oakland and Jackson counties, with the clusters in Ingham and Oakland counties appearing in 1950 and persisting to the present. Statistically significant focused clusters are found about the business address histories of 22 industries located in Oakland (19 clusters), Ingham (2) and Jackson (1) counties. Clusters in central and southeastern Oakland County appear in the 1930's and persist to the present day. These methods provide a systematic approach for evaluating a series of increasingly realistic alternative hypotheses regarding the sources of excess risk. So long as selection of cases and controls is population-based and not geographically biased, these tools can provide insights into geographic risk factors that were not specifically assessed in the case-control study design.

Case-control geographic clustering for residential histories accounting for risk factors and covariates

PubMed Central

2006-01-01

Background Methods for analyzing space-time variation in risk in case-control studies typically ignore residential mobility. We develop an approach for analyzing case-control data for mobile individuals and apply it to study bladder cancer in 11 counties in southeastern Michigan. At this time data collection is incomplete and no inferences should be drawn – we analyze these data to demonstrate the novel methods. Global, local and focused clustering of residential histories for 219 cases and 437 controls is quantified using time-dependent nearest neighbor relationships. Business address histories for 268 industries that release known or suspected bladder cancer carcinogens are analyzed. A logistic model accounting for smoking, gender, age, race and education specifies the probability of being a case, and is incorporated into the cluster randomization procedures. Sensitivity of clustering to definition of the proximity metric is assessed for 1 to 75 k nearest neighbors. Results Global clustering is partly explained by the covariates but remains statistically significant at 12 of the 14 levels of k considered. After accounting for the covariates 26 Local clusters are found in Lapeer, Ingham, Oakland and Jackson counties, with the clusters in Ingham and Oakland counties appearing in 1950 and persisting to the present. Statistically significant focused clusters are found about the business address histories of 22 industries located in Oakland (19 clusters), Ingham (2) and Jackson (1) counties. Clusters in central and southeastern Oakland County appear in the 1930's and persist to the present day. Conclusion These methods provide a systematic approach for evaluating a series of increasingly realistic alternative hypotheses regarding the sources of excess risk. So long as selection of cases and controls is population-based and not geographically biased, these tools can provide insights into geographic risk factors that were not specifically assessed in the case

7. Oblique view, east and north side, view to southwest. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Oblique view, east and north side, view to southwest. Note expansive use of glass to provide natural light to interior. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA

26. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Proposed 218-foot deck, plate-girder turn span, submitted by the Wisconsin Bridge and Iron Co., Milwaukee, Wisc. May 15 1914. Act size: approx. 23x34 in. Credit: columbus and Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

23. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Southern R'wy Co. in Ms. Repairs to Tombigbee River Bridge' gears and turn machinery. DWG # S. 30303. Scale: 1-1/2' = l'. July 21, 1913. Credit: Columbus & Greenville RR, Columbus, Ms. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

25. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Proposed 218-foot turn span, submitted by the American Bridge Co., Pittsburg, Pa., 15 May 1914. Inquiry # P-19242. Act. size: approx: 23x24 in. Credit: Columbus and Greenville RR, columbus, MS. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

MiRAR-miRNA Activity Reporter for Living Cells.

PubMed

Turk, Matthew A; Chung, Christina Z; Manni, Emad; Zukowski, Stephanie A; Engineer, Anish; Badakhshi, Yasaman; Bi, Yumin; Heinemann, Ilka U

2018-06-19

microRNA (miRNA) activity and regulation are of increasing interest as new therapeutic targets. Traditional approaches to assess miRNA levels in cells rely on RNA sequencing or quantitative PCR. While useful, these approaches are based on RNA extraction and cannot be applied in real-time to observe miRNA activity with single-cell resolution. We developed a green fluorescence protein (GFP)-based reporter system that allows for a direct, real-time readout of changes in miRNA activity in live cells. The miRNA activity reporter (MiRAR) consists of GFP fused to a 3′ untranslated region containing specific miRNA binding sites, resulting in miRNA activity-dependent GFP expression. Using qPCR, we verified the inverse relationship of GFP fluorescence and miRNA levels. We demonstrated that this novel optogenetic reporter system quantifies cellular levels of the tumor suppressor miRNA let-7 in real-time in single Human embryonic kidney 293 (HEK 293) cells. Our data shows that the MiRAR can be applied to detect changes in miRNA levels upon disruption of miRNA degradation pathways. We further show that the reporter could be adapted to monitor another disease-relevant miRNA, miR-122. With trivial modifications, this approach could be applied across the miRNome for quantification of many specific miRNA in cell cultures, tissues, or transgenic animal models.

Integrating Academic, Social, and Emotional Learning to Advance Equity and Achievement. Meeting 31 Summary (Oakland, California, December 6-7, 2016)

ERIC Educational Resources Information Center

Knudson, Joel

2016-01-01

The California Collaborative on District Reform convened in Oakland for a two-day meeting, "Integrating Academic, Social, and Emotional Learning to Advance Equity and Achievement." The meeting explored methods to support and integrate academic, social, and emotional learning in K-12 education. Implementation efforts surrounding the…

372. J.W.M., Delineator August 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

372. J.W.M., Delineator August 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6; SUPERSTRUCTURE - WEST BAY CROSSING; OUTSIDE ELEVATION OF HOUSING; CENTER ANCHORAGE - PIER NO. 4; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4854-XI; SHEET NO. E8 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Expedition_55_Education_In-Flight_Oakland_CC_Lake_Orion_HS_2018_107_1025_641759

NASA Image and Video Library

2018-04-18

SPACE STATION CREW MEMBERS DISCUSS LIFE IN SPACE WITH MICHIGAN STUDENTS----- Aboard the International Space Station, Expedition 55 Flight Engineers Drew Feustel and Ricky Arnold of NASA discussed life and research on the orbital outpost during an in-flight educational event April 17 with students from the Lake Orion (pron: OH-ree-on) High School and the Oakland Community College in Lake Orion, Michigan. Feustel, who is a native of Lake Orion, and Arnold, who is a former educator, launched to the station in late March for their mission on the orbital outpost.

Seismic Landslide Hazard for the Cities of Oakland and Piedmont, California

USGS Publications Warehouse

Miles, Scott B.; Keefer, David K.

2001-01-01

This map describes the possible hazard from earthquake-induced landslides for the cities of Oakland and Piedmont, CA. The hazard depicted by this map was modeled for a scenario corresponding to an M=7.1 earthquake on the Hayward, CA fault. This scenario magnitude is associated with complete rupture of the northern and southern segments of the Hayward fault, an event that has an estimated return period of about 500 years. The modeled hazard also corresponds to completely saturated ground-water conditions resulting from an extreme storm event or series of storm events. This combination of earthquake and ground-water scenarios represents a particularly severe state of hazard for earthquake-induced landslides. For dry ground-water conditions, overall hazard will be less, while relative patterns of hazard are likely to change.

MiRNA-miRNA synergistic network: construction via co-regulating functional modules and disease miRNA topological features.

PubMed

Xu, Juan; Li, Chuan-Xing; Li, Yong-Sheng; Lv, Jun-Ying; Ma, Ye; Shao, Ting-Ting; Xu, Liang-De; Wang, Ying-Ying; Du, Lei; Zhang, Yun-Peng; Jiang, Wei; Li, Chun-Quan; Xiao, Yun; Li, Xia

2011-02-01

Synergistic regulations among multiple microRNAs (miRNAs) are important to understand the mechanisms of complex post-transcriptional regulations in humans. Complex diseases are affected by several miRNAs rather than a single miRNA. So, it is a challenge to identify miRNA synergism and thereby further determine miRNA functions at a system-wide level and investigate disease miRNA features in the miRNA-miRNA synergistic network from a new view. Here, we constructed a miRNA-miRNA functional synergistic network (MFSN) via co-regulating functional modules that have three features: common targets of corresponding miRNA pairs, enriched in the same gene ontology category and close proximity in the protein interaction network. Predicted miRNA synergism is validated by significantly high co-expression of functional modules and significantly negative regulation to functional modules. We found that the MFSN exhibits a scale free, small world and modular architecture. Furthermore, the topological features of disease miRNAs in the MFSN are distinct from non-disease miRNAs. They have more synergism, indicating their higher complexity of functions and are the global central cores of the MFSN. In addition, miRNAs associated with the same disease are close to each other. The structure of the MFSN and the features of disease miRNAs are validated to be robust using different miRNA target data sets.

Novel internal regulators and candidate miRNAs within miR-379/miR-656 miRNA cluster can alter cellular phenotype of human glioblastoma.

PubMed

Nayak, Subhashree; Aich, Meghali; Kumar, Anupam; Sengupta, Suman; Bajad, Prajakta; Dhapola, Parashar; Paul, Deepanjan; Narta, Kiran; Purkrait, Suvendu; Mehani, Bharati; Suri, Ashish; Chakraborty, Debojyoti; Mukhopadhyay, Arijit; Sarkar, Chitra

2018-05-16

Clustered miRNAs can affect functioning of downstream pathways due to possible coordinated function. We observed 78-88% of the miR-379/miR-656 cluster (C14MC) miRNAs were downregulated in three sub-types of diffuse gliomas, which was also corroborated with analysis from The Cancer Genome Atlas (TCGA) datasets. The miRNA expression levels decreased with increasing tumor grade, indicating this downregulation as an early event in gliomagenesis. Higher expression of the C14MC miRNAs significantly improved glioblastioma prognosis (Pearson's r = 0.62; p < 3.08e-22). ENCODE meta-data analysis, followed by reporter assays validated existence of two novel internal regulators within C14MC. CRISPR activation of the most efficient internal regulator specifically induced members of the downstream miRNA sub-cluster and apoptosis in glioblastoma cells. Luciferase assays validated novel targets for miR-134 and miR-485-5p, two miRNAs from C14MC with the most number of target genes relevant for glioma. Overexpression of miR-134 and miR-485-5p in human glioblastoma cells suppressed invasion and proliferation, respectively. Furthermore, apoptosis was induced by both miRs, individually and in combination. The results emphasize the tumor suppressive role of C14MC in diffuse gliomas, and identifies two specific miRNAs with potential therapeutic value and towards better disease management and therapy.

75 FR 13576 - Labor Surplus Area Classification Under Executive Orders 12073 and 10582

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-22

.... Crawford County, MI Crawford County, MI. Delta County, MI Delta County, MI. Detroit city, MI Wayne County..., MN Chisago County, MN. Clearwater County, MN Clearwater County, MN. Crow Wing County, MN Crow Wing...

miRNAmeConverter: an R/bioconductor package for translating mature miRNA names to different miRBase versions.

PubMed

Haunsberger, Stefan J; Connolly, Niamh M C; Prehn, Jochen H M

2017-02-15

The miRBase database is the central and official repository for miRNAs and the current release is miRBase version 21.0. Name changes in different miRBase releases cause inconsistencies in miRNA names from version to version. When working with only a small number of miRNAs the translation can be done manually. However, with large sets of miRNAs, the necessary correction of such inconsistencies becomes burdensome and error-prone. We developed miRNAmeConverter , available as a Bioconductor R package and web interface that addresses the challenges associated with mature miRNA name inconsistencies. The main algorithm implemented enables high-throughput automatic translation of species-independent mature miRNA names to user selected miRBase versions. The web interface enables users less familiar with R to translate miRNA names given in form of a list or embedded in text and download of the results. The miRNAmeConverter R package is open source under the Artistic-2.0 license. It is freely available from Bioconductor ( http://bioconductor.org/packages/miRNAmeConverter ). The web interface is based on R Shiny and can be accessed under the URL http://www.systemsmedicineireland.ie/tools/mirna-name-converter/ . The database that miRNAmeConverter depends on is provided by the annotation package miRBaseVersions.db and can be downloaded from Bioconductor ( http://bioconductor.org/packages/miRBaseVersions.db ). Minimum R version 3.3.0 is required. stefanhaunsberger@rcsi.ie. Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

5. Oblique view, west end and south side, view to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Oblique view, west end and south side, view to northeast. Shed-roofed entries on south side of lean-to will be replaced in this project to provide handicap access. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA

Oakland Readers. A Book of Life Stories Told by Students in the Second Start Adult Literacy Program. Levels One-Four.

ERIC Educational Resources Information Center

Lamb, Jessica, Ed.

This set of Oakland Readers consists of four books of oral histories edited on four reading levels. Each book contains life stories told by students in the Second Start Adult Literacy Program. The books are intended for use by tutors and adult students/new readers in adult literacy programs. Life stories of eight students appear in each book. In…

MiRGOFS: A GO-based functional similarity measure for miRNAs, with applications to the prediction of miRNA subcellular localization and miRNA-disease association.

PubMed

Yang, Yang; Fu, Xiaofeng; Qu, Wenhao; Xiao, Yiqun; Shen, Hong-Bin

2018-04-27

Benefiting from high-throughput experimental technologies, whole-genome analysis of microRNAs (miRNAs) has been more and more common to uncover important regulatory roles of miRNAs and identify miRNA biomarkers for disease diagnosis. As a complementary information to the high-throughput experimental data, domain knowledge like the Gene Ontology and KEGG pathway is usually used to guide gene function analysis. However, functional annotation for miRNAs is scarce in the public databases. Till now, only a few methods have been proposed for measuring the functional similarity between miRNAs based on public annotation data, and these methods cover a very limited number of miRNAs, which are not applicable to large-scale miRNA analysis. In this paper, we propose a new method to measure the functional similarity for miRNAs, called miRGOFS, which has two notable features: I) it adopts a new GO semantic similarity metric which considers both common ancestors and descendants of GO terms; II) it computes similarity between GO sets in an asymmetric manner, and weights each GO term by its statistical significance. The miRGOFS-based predictor achieves an F1 of 61.2% on a benchmark data set of miRNA localization, and AUC values of 87.7% and 81.1% on two benchmark sets of miRNA-disease association, respectively. Compared with the existing functional similarity measurements of miRNAs, miRGOFS has the advantages of higher accuracy and larger coverage of human miRNAs (over 1000 miRNAs). http://www.csbio.sjtu.edu.cn/bioinf/MiRGOFS/. yangyang@cs.sjtu.edu.cn or hbshen@sjtu.edu.cn. Supplementary data are available at Bioinformatics online.

miRegulome: a knowledge-base of miRNA regulomics and analysis.

PubMed

Barh, Debmalya; Kamapantula, Bhanu; Jain, Neha; Nalluri, Joseph; Bhattacharya, Antaripa; Juneja, Lucky; Barve, Neha; Tiwari, Sandeep; Miyoshi, Anderson; Azevedo, Vasco; Blum, Kenneth; Kumar, Anil; Silva, Artur; Ghosh, Preetam

2015-08-05

miRNAs regulate post transcriptional gene expression by targeting multiple mRNAs and hence can modulate multiple signalling pathways, biological processes, and patho-physiologies. Therefore, understanding of miRNA regulatory networks is essential in order to modulate the functions of a miRNA. The focus of several existing databases is to provide information on specific aspects of miRNA regulation. However, an integrated resource on the miRNA regulome is currently not available to facilitate the exploration and understanding of miRNA regulomics. miRegulome attempts to bridge this gap. The current version of miRegulome v1.0 provides details on the entire regulatory modules of miRNAs altered in response to chemical treatments and transcription factors, based on validated data manually curated from published literature. Modules of miRegulome (upstream regulators, downstream targets, miRNA regulated pathways, functions, diseases, etc) are hyperlinked to an appropriate external resource and are displayed visually to provide a comprehensive understanding. Four analysis tools are incorporated to identify relationships among different modules based on user specified datasets. miRegulome and its tools are helpful in understanding the biology of miRNAs and will also facilitate the discovery of biomarkers and therapeutics. With added features in upcoming releases, miRegulome will be an essential resource to the scientific community. http://bnet.egr.vcu.edu/miRegulome.

24. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. RAILROAD, RR BRIDGE MISSISSIPPI, CLAY CO., WAVERLY 1.5 mi. S of MS. 50 Southern R'wy; Co., in Ms. Present and proposed draw bridge over Tombigbee River.' Elevations and Plan, with falsework. Wisconsin Bridge and Iron Co., Milwaukee, Wisc., DWG #S. 3-303. Last Revision, Jan. 11 1915. Act. size: 23x34 in. Credit: Columbus and Greenville Rr, Columbus, Ms. Sarcone Photography, Columbus, MS. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

miREE: miRNA recognition elements ensemble

PubMed Central

2011-01-01

Background Computational methods for microRNA target prediction are a fundamental step to understand the miRNA role in gene regulation, a key process in molecular biology. In this paper we present miREE, a novel microRNA target prediction tool. miREE is an ensemble of two parts entailing complementary but integrated roles in the prediction. The Ab-Initio module leverages upon a genetic algorithmic approach to generate a set of candidate sites on the basis of their microRNA-mRNA duplex stability properties. Then, a Support Vector Machine (SVM) learning module evaluates the impact of microRNA recognition elements on the target gene. As a result the prediction takes into account information regarding both miRNA-target structural stability and accessibility. Results The proposed method significantly improves the state-of-the-art prediction tools in terms of accuracy with a better balance between specificity and sensitivity, as demonstrated by the experiments conducted on several large datasets across different species. miREE achieves this result by tackling two of the main challenges of current prediction tools: (1) The reduced number of false positives for the Ab-Initio part thanks to the integration of a machine learning module (2) the specificity of the machine learning part, obtained through an innovative technique for rich and representative negative records generation. The validation was conducted on experimental datasets where the miRNA:mRNA interactions had been obtained through (1) direct validation where even the binding site is provided, or through (2) indirect validation, based on gene expression variations obtained from high-throughput experiments where the specific interaction is not validated in detail and consequently the specific binding site is not provided. Conclusions The coupling of two parts: a sensitive Ab-Initio module and a selective machine learning part capable of recognizing the false positives, leads to an improved balance between

About miRNAs, miRNA seeds, target genes and target pathways.

PubMed

Kehl, Tim; Backes, Christina; Kern, Fabian; Fehlmann, Tobias; Ludwig, Nicole; Meese, Eckart; Lenhof, Hans-Peter; Keller, Andreas

2017-12-05

miRNAs are typically repressing gene expression by binding to the 3' UTR, leading to degradation of the mRNA. This process is dominated by the eight-base seed region of the miRNA. Further, miRNAs are known not only to target genes but also to target significant parts of pathways. A logical line of thoughts is: miRNAs with similar (seed) sequence target similar sets of genes and thus similar sets of pathways. By calculating similarity scores for all 3.25 million pairs of 2,550 human miRNAs, we found that this pattern frequently holds, while we also observed exceptions. Respective results were obtained for both, predicted target genes as well as experimentally validated targets. We note that miRNAs target gene set similarity follows a bimodal distribution, pointing at a set of 282 miRNAs that seems to target genes with very high specificity. Further, we discuss miRNAs with different (seed) sequences that nonetheless regulate similar gene sets or pathways. Most intriguingly, we found miRNA pairs that regulate different gene sets but similar pathways such as miR-6886-5p and miR-3529-5p. These are jointly targeting different parts of the MAPK signaling cascade. The main goal of this study is to provide a general overview on the results, to highlight a selection of relevant results on miRNAs, miRNA seeds, target genes and target pathways and to raise awareness for artifacts in respective comparisons. The full set of information that allows to infer detailed results on each miRNA has been included in miRPathDB, the miRNA target pathway database (https://mpd.bioinf.uni-sb.de).

miRiadne: a web tool for consistent integration of miRNA nomenclature.

PubMed

Bonnal, Raoul J P; Rossi, Riccardo L; Carpi, Donatella; Ranzani, Valeria; Abrignani, Sergio; Pagani, Massimiliano

2015-07-01

The miRBase is the official miRNA repository which keeps the annotation updated on newly discovered miRNAs: it is also used as a reference for the design of miRNA profiling platforms. Nomenclature ambiguities generated by loosely updated platforms and design errors lead to incompatibilities among platforms, even from the same vendor. Published miRNA lists are thus generated with different profiling platforms that refer to diverse and not updated annotations. This greatly compromises searches, comparisons and analyses that rely on miRNA names only without taking into account the mature sequences, which is particularly critic when such analyses are carried over automatically. In this paper we introduce miRiadne, a web tool to harmonize miRNA nomenclature, which takes into account the original miRBase versions from 10 up to 21, and annotations of 40 common profiling platforms from nine brands that we manually curated. miRiadne uses the miRNA mature sequence to link miRBase versions and/or platforms to prevent nomenclature ambiguities. miRiadne was designed to simplify and support biologists and bioinformaticians in re-annotating their own miRNA lists and/or data sets. As Ariadne helped Theseus in escaping the mythological maze, miRiadne will help the miRNA researcher in escaping the nomenclature maze. miRiadne is freely accessible from the URL http://www.miriadne.org. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

miRNA-15a, miRNA-15b, and miRNA-499 are Reduced in Erythrocytes of Pre-Diabetic African-American Adults.

PubMed

Fluitt, Maurice B; Kumari, Namita; Nunlee-Bland, Gail; Nekhai, Sergei; Gambhir, Kanwal K

2016-12-01

The use of circulatory miRNAs as biomarkers and therapeutic targets for T2DM is an explosive area of study. However, no study has investigated circulatory miRNA expression exclusively in African-American adults. The aim of this study was to identify the expression of nine selected miRNAs in erythrocytes of pre-diabetic and type 2 diabetic African-American adults. Patients were recruited from the Howard University Hospital Diabetes Treatment Center following an 8 to 10 hour overnight fast. Expression of the nine selected miRNAs (miRNA-499, miRNA-146, miRNA-126, miRNA-223, miRNA-15a, miRNA-15b, miRNA-224, miRNA-326, and miRNA-375) was evaluated using quantitative real time PCR. miRNA-15a, miRNA-15b, and miRNA-499 were significantly reduced in erythrocytes of pre-diabetic African-American adults. In the T2DM group, we found significant correlations between miRNA-15a and BMI (r=0.59, p=0.04), miRNA-15a and weight (r=0.52, p=0.01), and miRNA-15b and diastolic blood pressure (r=-0.52, p=0.02). In the pre-diabetic group, we found significant correlations between miRNA-15b and weight (r=0.90, p=0.02) and miRNA-499 and HbA1c (r=-0.89, p=0.01). To our knowledge, this is the first study investigating miRNA expression in erythrocytes of non-diabetic high-risk obese--pre-diabetic and type 2 diabetic African-American adults. The findings of this study are consistent with previous reports of reduced expression of miRNA-15a, miRNA-15b, and miRNA-499 in human plasma or serum and in animal models. The current findings support the use of circulating miRNA-15a, miRNA-15b, and miRNA-499 as potential biomarkers for T2DM in African-American adults.

miRNA-15a, miRNA-15b, and miRNA-499 are Reduced in Erythrocytes of Pre-Diabetic African-American Adults

PubMed Central

Fluitt, Maurice B.; Kumari, Namita; Nunlee-Bland, Gail; Nekhai, Sergei; Gambhir, Kanwal K.

2017-01-01

Aims The use of circulatory miRNAs as biomarkers and therapeutic targets for T2DM is an explosive area of study. However, no study has investigated circulatory miRNA expression exclusively in African-American adults. The aim of this study was to identify the expression of nine selected miRNAs in erythrocytes of pre-diabetic and type 2 diabetic African-American adults. Main Methods Patients were recruited from the Howard University Hospital Diabetes Treatment Center following an 8 to 10 hour overnight fast. Expression of the nine selected miRNAs (miRNA-499, miRNA-146, miRNA-126, miRNA-223, miRNA-15a, miRNA-15b, miRNA-224, miRNA-326, and miRNA-375) was evaluated using quantitative real time PCR. Key Findings miRNA-15a, miRNA-15b, and miRNA-499 were significantly reduced in erythrocytes of pre-diabetic African-American adults. In the T2DM group, we found significant correlations between miRNA-15a and BMI (r=0.59, p=0.04), miRNA-15a and weight (r=0.52, p=0.01), and miRNA-15b and diastolic blood pressure (r=−0.52, p=0.02). In the pre-diabetic group, we found significant correlations between miRNA-15b and weight (r=0.90, p=0.02) and miRNA-499 and HbA1c (r=−0.89, p=0.01). Significance To our knowledge, this is the first study investigating miRNA expression in erythrocytes of non-diabetic high-risk obese--pre-diabetic and type 2 diabetic African-American adults. The findings of this study are consistent with previous reports of reduced expression of miRNA-15a, miRNA-15b, and miRNA-499 in human plasma or serum and in animal models. The current findings support the use of circulating miRNA-15a, miRNA-15b, and miRNA-499 as potential biomarkers for T2DM in African-American adults. PMID:29399662

Suspension Bridge Structural Systems: Cable Suspension & Anchorage; Warren Stiffening ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Suspension Bridge Structural Systems: Cable Suspension & Anchorage; Warren Stiffening Truss; Upper & Lower Decks; Assembled System - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Identification of Four Oxidative Stress-Responsive MicroRNAs, miR-34a-5p, miR-1915-3p, miR-638, and miR-150-3p, in Hepatocellular Carcinoma.

PubMed

Wan, Yong; Cui, Ruixia; Gu, Jingxian; Zhang, Xing; Xiang, Xiaohong; Liu, Chang; Qu, Kai; Lin, Ting

2017-01-01

Increasing evidence suggests that oxidative stress plays an essential role during carcinogenesis. However, the underlying mechanism between oxidative stress and carcinogenesis remains unknown. Recently, microRNAs (miRNAs) are revealed to be involved in oxidative stress response and carcinogenesis. This study aims to identify miRNAs in hepatocellular carcinoma (HCC) cells which might involve in oxidative stress response. An integrated analysis of miRNA expression signature was performed by employing robust rank aggregation (RRA) method, and four miRNAs (miR-34a-5p, miR-1915-3p, miR-638, and miR-150-3p) were identified as the oxidative stress-responsive miRNAs. Pathway enrichment analysis suggested that these four miRNAs played an important role in antiapoptosis process. Our data also revealed miR-34a-5p and miR-1915-3p, but not miR-150-3p and miR-638, were regulated by p53 in HCC cell lines under oxidative stress. In addition, clinical investigation revealed that these four miRNAs might be involved in oxidative stress response by targeting oxidative stress-related genes in HCC tissues. Kaplan-Meier analysis showed that these four miRNAs were associated with patients' overall survival. In conclusion, we identified four oxidative stress-responsive miRNAs, which were regulated by p53-dependent (miR-34a-5p and miR-1915-3p) and p53-independent pathway (miR-150-3p and miR-638). These four miRNAs may offer new strategy for HCC diagnosis and prognosis.

29. VIEW OF 4TH FLOOR'S TELEPHONE RACKS WITH CABLE TRAYS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. VIEW OF 4TH FLOOR'S TELEPHONE RACKS WITH CABLE TRAYS ABOVE. THESE ARE NEWER APPARATUS AND NOT ORIGINAL. - Pacific Telephone & Telegraph Company Building, 1519 Franklin Street, Oakland, Alameda County, CA

26. Photocopy of photograph (original print in files of Milton ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. Photocopy of photograph (original print in files of Milton T. Pflueger, AIA) Gabriel Moulin Studios, Photographer 1932 WOMEN'S SMOKING ROOM IN BASEMENT - Paramount Theatre, 2025 Broadway, Oakland, Alameda County, CA

29. Photocopy of photograph (original print in files of Milton ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. Photocopy of photograph (original print in files of Milton T. Pflueger, AIA) Gabriel Moulin Studios, Photographer 1932 COSMETIC ROOM, WOMEN'S MEZZANINE LOUNGE - Paramount Theatre, 2025 Broadway, Oakland, Alameda County, CA

Chinese Connections

ERIC Educational Resources Information Center

Skilling, William C.

2012-01-01

When L. Brooks Patterson, the executive of Oakland County, Michigan, publicly called for the county to become the first in America to teach Mandarin Chinese in every public school district, the Oxford Community Schools responded immediately. Over the past four years, the school district of 5,030 students in southeastern Michigan has elevated the…

Computational miRNomics.

PubMed

Allmer, Jens; Yousef, Malik

2016-12-01

Editorial The term MicroRNA or its contraction miRNA currently appears in 21,215 titles of abstracts, published between 1997 and now, available on Pubmed (2016-21-22:12:59 EET). 4,108 of these were published in 2016 alone which signifies the importance of miRNA-related research. MicroRNAs can be detected experimentally using various techniques like directional cloning of endogenous small RNAs but they are time consuming [1]. Additionally, it is necessary for the miRNA and its mRNA target(s) to be co-expressed to infer a functional relationship which is difficult, if not impossible, to achieve [2]. Since experimental approaches are facing such difficulties, they have been complemented by computational approaches [3] thereby defining the field of computational miRNomics. Due to the rapid development in the discipline, it is important to assess the state-of-the-art. In this special issue, several areas of the field are investigated ranging from pre-miRNA detection via machine learning to application of differential expression analysis in plants. First, Saçar Demirci et al. discuss an approach to virus pre-miRNA detection using machine learning [4]. Such approaches are based on parameterization of miRNAs and Yousef et al. discuss how to select among such features [5]. A different computational perspective is provided by Kotipalli et al. who model the kinetics of miRNA genesis and targeting [6]. To fuel more refined future models for genesis and targeting, it is important to establish miRNA and target expression under varying conditions. Zhang et al. [7] and Kanke et al. [8] discuss two approaches to quantify miRNAs and other non-coding short RNAs. Diler et al., finally, discuss actual biological implications of differentially expressed miRNAs [9]. This special issue on computational miRNomics, thus, provides a trajectory from detection of pre-miRNAs to biological implications of differentially expressed miRNAs. Additional topics will be covered in the upcoming second

The Oakland Conglomerate: a Hayward Fault Teconite?

NASA Astrophysics Data System (ADS)

Strayer, L. M.; Allen, J. R.

2008-12-01

The Late Cretaceous Oakland Conglomerate (OC), a coarse-grained cobble and sandstone unit of the Great Valley Sequence is a tectonite. Faulted and shattered cobbles and well developed grain-on-grain contact features between clasts are ubiquitous and penetrative throughout conglomeratic lenses. The OC outcrops east of the Hayward fault (HF) and adjacent to the Chabot fault in the East Bay Hills. It overlies the Knoxville Formation and may have been buried beneath 4-6 km of younger units. The OC is a proximal submarine fan deposit with sediment sourced to the ancestral Klamath and Sierra Nevada. Clast types are dominated by volcanics, granitoids, as well as numerous quartzites, perhaps reflecting complex provenance:Klamath and pre-Sierran arc and pre-Cretaceous Basin and Range. And although there was a significant interval between the Late-K deposition of the OC and the inception of San Andreas faulting in the Bay Area, its 1-2 km proximity to the HF in the Oakland Metropolitan area strongly suggests that much of the brittle-plastic deformation within the OC may be due to earthquakes upon the nearby Hayward fault. Clasts with the OC are frequently shattered, fractured or faulted. Most have grain-on-grain contact features on their surfaces regardless of whether they are matrix or grain supported. Faulting in the cobbles ranges from outcrop scale, penetrative and often conjugate shear fracture sets that run through both cobbles and matrix (if present), to closely spaced en-echelon faults that clearly deform cobbles, and radially shattered specimens with nearly conical conjugate shear fractures that are clearly the result of point loading due to grain-on-grain contact. There are at least 3 types of contact structures, ranging from: 1) Type-H, bright circular halos with little or no surface dimpling, likely the result of intense microfracture at the contact; 2) Type-S, shattered, rounded 'firing-pin' structures that have pulverized, depressed contact that is the locus of

22. TYPICAL FOR THE FIRST FLOOR INTERIORS, ARE PAIRED COLUMN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. TYPICAL FOR THE FIRST FLOOR INTERIORS, ARE PAIRED COLUMN PILASTERS IN KEENE CEMENT PLASTER. BASE OF PILASTER IS SHOWN. - Pacific Telephone & Telegraph Company Building, 1519 Franklin Street, Oakland, Alameda County, CA

The PTTG1-targeting miRNAs miR-329, miR-300, miR-381, and miR-655 inhibit pituitary tumor cell tumorigenesis and are involved in a p53/PTTG1 regulation feedback loop

PubMed Central

Diao, Cai-feng; Li, Jian-wei; Su, Jing-liang; Zhang, Sai

2015-01-01

Deregulation of the pituitary tumor transforming gene (PTTG1), a newly discovered oncogene, is a hallmark of various malignancies, including pituitary tumors. However, the mechanisms regulating PTTG1 expression are still needed to be explored. MicroRNAs (miRNAs) are a novel class of small RNA molecules that act as posttranscriptional regulators of gene expression and can play a significant role in tumor development. Here, we identified a series of miRNAs, namely, miR-329, miR-300, miR-381 and miR-655, which could target PTTG1 messenger RNA and inhibit its expression. Interestingly, all four miRNAs significantly that are downregulated in pituitary tumors were mapped to the 14q32.31 locus, which acts as a tumor suppressor in several cancers. Functional studies show that the PTTG1-targeting miRNAs inhibit proliferation, migration and invasion but induce apoptosis in GH3 and MMQ cells. Furthermore, overexpression of a PTTG1 expression vector lacking the 3′UTR partially reverses the tumor suppressive effects of these miRNAs. Next, we identified the promoter region of PTTG1-targeting miRNAs with binding sites for p53. In our hands, p53 transcriptionally activated the expression of these miRNAs in pituitary tumor cells. Finally, we found that PTTG1 could inhibit p53 transcriptional activity to the four miRNAs. These data indicate the existence of a feedback loop between PTTG1 targeting miRNAs, PTTG1 and p53 that promotes pituitary tumorigenesis. Together, these findings suggest that these PTTG1-targeting miRNAs are important players in the regulation of pituitary tumorigenesis and that these miRNAs may serve as valuable therapeutic targets for cancer treatment. PMID:26320179

The association of circulating miR-30a, miR-29 and miR-133 with white-coat hypertension.

PubMed

Huang, Yu-Qing; Huang, Cheng; Chen, Ji-Yan; Li, Jie; Feng, Ying-Qing

2016-12-01

The aim of the present study was to investigate the association of circulating miRNAs with white-coat hypertension (WCH) and further analyze whether miRNAs could be as potential biomarkers for WCH. Quantitative reverse transcriptase PCR (qRT-PCR) was used to evaluate the expression of selected miRNAs. The area under the receiver-operating characteristic curve was used to evaluate diagnostic accuracy. MiR-30a yielded an AUC of 0.984 (95% CI: 0.001-1.00; p < 0.001) and 0.816 (95% CI: 0.718-0.915; p < 0.001); miR-29 yielded an AUC of 0.955 (95% CI: 0.913-0.998; p < 0.001) and 0.799 (95% CI: 0.697-0.902; p < 0.001); miR-133 yielded an AUC of 0.949 (95% CI: 0.900-0.999; p < 0.001) and 0.713 (95% CI: 0.593-0.834; p < 0.001), respectively. The study suggested that miR-30a, miR-29 and miR-133 have great potential to be noninvasive screening tools for WCH detection.

miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.).

PubMed

Dmitriev, Alexey A; Kudryavtseva, Anna V; Bolsheva, Nadezhda L; Zyablitsin, Alexander V; Rozhmina, Tatiana A; Kishlyan, Natalya V; Krasnov, George S; Speranskaya, Anna S; Krinitsina, Anastasia A; Sadritdinova, Asiya F; Snezhkina, Anastasiya V; Fedorova, Maria S; Yurkevich, Olga Yu; Muravenko, Olga V; Belenikin, Maxim S; Melnikova, Nataliya V

2017-01-01

Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax ( Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl 3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390-TAS3 and GRF5, and miR393-AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants.

Water Quality Investigations at Lake Merritt in Oakland, California

NASA Astrophysics Data System (ADS)

Carter, G.; Casino, C.; Johnson, K.; Huang, J.; Le, A.; Truisi, V. M.; Turner, D.; Yanez, F.; Yu, J. F.; Unigarro, M.; Vue, G.; Garduno, L.; Cuff, K.

2005-12-01

Lake Merritt is a saltwater tidal lagoon that forms a portion of a wildlife refuge in downtown Oakland, California. The general area was designated as the nation's first wildlife refuge in 1869, and is currently the home to over 90 species of migrating waterfowl, as well as a variety of aquatic wildlife. Situated within an area composed of compacted marine sediment located near the center of Oakland, Lake Merritt also serves as a major local catchment basin, receiving significant urban runoff from a 4,650 acre local watershed through 60 storm drains and four culverted creeks. Due to factors related to its geographical location, Lake Merritt has suffered from poor water quality at various times throughout its history. In fact, in May of 1999 the US Environmental Protection Agency designated Lake Merritt as a body of water whose beneficial uses are impaired, mainly due to high levels of trash and low levels of dissolved oxygen. As a contribution to continuing efforts to monitor and assess water quality of the Lake, we began a water quality investigation during the Summer of 2005, which included the measurement of dissolved oxygen concentrations of samples collected near its surface at over 85 different locations. These measurements were made using a sensor attached to a PASCO data- logger. The sensor measures the electric current produced by a chemical reaction in its probe, which is composed of a platinum cathode and a silver anode surrounded by an electrolyte solution. Results of these measurements were statistically analyzed, mapped, and then used in assessing the quality of Lake Merritt's water, particularly in relation to supporting aquatic biota. Preliminary analysis of results obtained so far indicates that the highest quality waters in Lake Merritt occur in areas that are closest to a source of San Francisco Bay water, as well as those areas nearby where water circulation is robust. Significantly high levels of dissolved oxygen were measured in an area that

8. Detail, typical shedroofed entry on south side. The current ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Detail, typical shed-roofed entry on south side. The current project will replace these with similar structures that will allow handicap access to the lean-to portion of the building containing offices, restrooms, and other employee spaces. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA

miRTex: A Text Mining System for miRNA-Gene Relation Extraction

PubMed Central

Li, Gang; Ross, Karen E.; Arighi, Cecilia N.; Peng, Yifan; Wu, Cathy H.; Vijay-Shanker, K.

2015-01-01

MicroRNAs (miRNAs) regulate a wide range of cellular and developmental processes through gene expression suppression or mRNA degradation. Experimentally validated miRNA gene targets are often reported in the literature. In this paper, we describe miRTex, a text mining system that extracts miRNA-target relations, as well as miRNA-gene and gene-miRNA regulation relations. The system achieves good precision and recall when evaluated on a literature corpus of 150 abstracts with F-scores close to 0.90 on the three different types of relations. We conducted full-scale text mining using miRTex to process all the Medline abstracts and all the full-length articles in the PubMed Central Open Access Subset. The results for all the Medline abstracts are stored in a database for interactive query and file download via the website at http://proteininformationresource.org/mirtex. Using miRTex, we identified genes potentially regulated by miRNAs in Triple Negative Breast Cancer, as well as miRNA-gene relations that, in conjunction with kinase-substrate relations, regulate the response to abiotic stress in Arabidopsis thaliana. These two use cases demonstrate the usefulness of miRTex text mining in the analysis of miRNA-regulated biological processes. PMID:26407127

Deficient Differentiation of Mast Cells in the Skin of mi/mi Mice

PubMed Central

Kasugai, Tsutomu; Oguri, Kayoko; Jippo-Kanemoto, Tomoko; Morimoto, Masahiro; Yamatodani, Atsushi; Yoshida, Keiichi; Ebi, Yoshitaka; Isozaki, Koji; Tei, Hideki; Tsujimura, Tohru; Nomura, Shintaro; Okayama, Minoru; Kitamura, Yukihiko

1993-01-01

The staining property of skin mast cells changed from Alcian blue+/berberine sulfate- to Alcian blue +/berberine sulfate+ in the skin of normal (+/+) and Wv/Wv mice. In contrast, this change did not occur in the skin of mi/mi mice. Heparin content and histamine content per a mi/mi skin mast cell were estimated to be 34% and 18% those of a +/+ skin mast cell, respectively. The low heparin content of mi/mi skin mast cells seemed to be consistent with the Alcian blue+/berberine sulfate- staining property. Expression of genes encoding mast cell-specific proteolytic enzymes was examined by Northern blotting and in situ hybridization. Messenger RNA of mast cell carboxypeptidase A was expressed most of all by +/+, WV Wv/W+ and mi/mi skin mast cells, but mRNA of mouse mast cell protease (MMCP)-6 was expressed by approximately a half of +/+ and Wv/Wv skin mast cells and by only 3% of mi/mi skin mast cells. A significant amount of MMCP-2 mRNA was not expressed in the skin of all +/+, Wv/Wv and mi/mi mice. This shows the presence of at least three phenotypes in skin mast cells of mice: berberine sulfate+/MMCP-6+, berberine sulfate+/MMCP-6-, and berberine sulfate-/ MMCP-6-. The in situ hybridization of mRNA of mast cell-specific proteolytic enzymes seemed to be useful to describe abnormalities of mast cell differentiation in the skin of mi/mi mice. ImagesFigure 4Figure 5 PMID:8238251

MicroRNAs modulating angiogenesis: miR-129-1 and miR-133 act as angio-miR in HUVECs.

PubMed

Soufi-Zomorrod, Mina; Hajifathali, Abbas; Kouhkan, Fatemeh; Mehdizadeh, Mahshid; Rad, Seyed Mohammad Ali Hosseini; Soleimani, Masoud

2016-07-01

The sprouting of new blood vessels by angiogenesis is critical in vascular development and homeostasis. Aberrant angiogenesis leads to enormous pathological conditions such as ischemia and cancer. MicroRNAs (also known as miRNAs or miRs) play key roles in regulation of a range of cellular processes by posttranscriptional suppression of their target genes. Recently, new studies have indicated that miRNAs are involved in certain angiogenic settings and signaling pathways use these non-coding RNAs to promote or suppress angiogenic processes. Herein, VEGFR2 and FGFR1 were identified as miR-129-1 and miR-133 targets using bioinformatic algorithms, respectively. Afterwards, using luciferase reporter assay and gene expression analysis at both mRNA and protein levels, VEGFR2 and FGFR1 were validated as miR-129-1 and miR-133 targets. In addition, we showed that miR-129-1 and miR-133 suppress angiogenesis properties such as proliferation rate, cell viability, and migration activity of human umbilical vein endothelial cells (HUVEC) in vitro. We conclude that these miRNAs can suppress key factors of angiogenesis by directly targeting them. These results have important therapeutic implications for a variety of diseases involving deregulation of angiogenesis, including cancer.

3. Historic American Buildings Survey Souther Pacific Railroad Coll. Wharf ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Historic American Buildings Survey Souther Pacific Railroad Coll. Wharf - Opened January 16, 1871 Painting of the Long Wharf - Opened January 16, 1871 - Southern Pacific Mole & Pier, Seventh Street, Oakland, Alameda County, CA

miR-335 and miR-363 regulation of neuroblastoma tumorigenesis and metastasis.

PubMed

Qiao, Jingbo; Lee, Sora; Paul, Pritha; Theiss, Lauren; Tiao, Joshua; Qiao, Lan; Kong, Andrew; Chung, Dai H

2013-08-01

microRNA (miRNA) functions broadly as post-transcriptional regulators of gene expression, and disproportionate miRNAs can result in dysregulation of oncogenes in cancer cells. We have previously shown that gastrin-releasing peptide receptor (GRP-R) signaling regulates tumorigenicity of neuroblastoma cells. Herein, we sought to characterize miRNA profile in GRP-R silenced neuroblastoma cells, and to determine the role of miRNAs on tumorigenicity and metastatic potential. Human neuroblastoma cell lines, BE(2)-C and SK-N-SH, were used for our study. Stably transfected GRP-R silenced cells were assessed for miRNA profiles. Cells were transfected with miR-335, miR-363, or miR-CON, a nontargeting control, and in vitro assays were performed. In vivo functions of miR-335 and miR-363 were also assessed in a spleen-liver metastasis murine model. GRP-R silencing significantly increased expression of miR-335 and miR-363 in BE(2)-C cells. Overexpression of miR-335 and miR-363 decreased tumorigenicity as measured by clonogenicity, anchorage-independent growth, and metastasis determined by cell invasion assay and liver metastasis in vivo. We report, for the first time, that GRP-R-mediated tumorigenicity and increased metastatic potential in neuroblastoma are regulated, in part, by miR-335 and miR-363. A better understanding of the anti-tumor functions of miRNAs could provide valuable insights to discerning molecular mechanisms responsible for neuroblastoma metastasis. Copyright © 2013 Mosby, Inc. All rights reserved.

miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.)

PubMed Central

Zyablitsin, Alexander V.; Rozhmina, Tatiana A.; Speranskaya, Anna S.; Sadritdinova, Asiya F.

2017-01-01

Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390–TAS3 and GRF5, and miR393–AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants. PMID:28299328

Role of miR-27a, miR-181a and miR-20b in gastric cancer hypoxia-induced chemoresistance

PubMed Central

Danza, Katia; Silvestris, Nicola; Simone, Giovanni; Signorile, Michele; Saragoni, Luca; Brunetti, Oronzo; Monti, Manlio; Mazzotta, Annalisa; De Summa, Simona; Mangia, Anita; Tommasi, Stefania

2016-01-01

ABSTRACT Despite the search for new therapeutic strategies for gastric cancer (GC), there is much evidence of progression due to resistance to chemotherapy. Multidrug resistance (MDR) is the ability of cancer cells to survive after exposure to chemotherapeutic agents. The involvement of miRNAs in the development of MDR has been well described but miRNAs able to modulate the sensitivity to chemotherapy by regulating hypoxia signaling pathways have not yet been fully addressed in GC. Our aim was to analyze miR-20b, miR-27a and miR-181a expression with respect to (epirubicin/oxaliplatin/capecitabine (EOX)) chemotherapy regimen in a set of GC patients, in order to investigate whether miRNAs deregulation may influence GC MDR also via hypoxia signaling modulation. Cancer biopsy were obtained from 21 untreated HER2 negative advanced GC patients, retrospectively analyzed. All patients received a first-line chemotherapy (EOX) regimen. MirWalk database was used to identify miR-27a, miR-181a and miR-20b target genes. The expression of miRNAs and of HIPK2, HIF1A and MDR1 genes were detected by real-time PCR. HIPK2 localization was assessed by immunohistochemistry. Our data showed the down-regulation of miR-20b, miR-27a, miR-181a concomitantly to higher levels of MDR1, HIF1A and HIPK2 genes in GC patients with a progressive disease respect to those with a disease control rate. Moreover, immunohistochemistry assay highlighted a higher cytoplasmic HIPK2 staining, suggesting a different role for it. We showed that aberrant expression of miR-20b, miR27a and miR-181a was associated with chemotherapeutic response in GC through HIF1A, MDR1 and HIPK2 genes modulation, suggesting a possible novel therapeutic strategy. PMID:26793992

miR-192, miR-194 and miR-215: a convergent microRNA network suppressing tumor progression in renal cell carcinoma.

PubMed

Khella, H W Z; Bakhet, M; Allo, G; Jewett, M A S; Girgis, A H; Latif, A; Girgis, H; Von Both, I; Bjarnason, G A; Yousef, G M

2013-10-01

MicroRNAs (miRNAs) play a crucial role in tumor progression and metastasis. We, and others, recently identified a number of miRNAs that are dysregulated in metastatic renal cell carcinoma compared with primary renal cell carcinoma. Here, we investigated three miRNAs that are significantly downregulated in metastatic tumors: miR-192, miR-194 and miR-215. Gain-of-function analyses showed that restoration of their expression decreases cell migration and invasion in renal cell carcinoma cell line models, whereas knockdown of these miRNAs resulted in enhancing cellular migration and invasion abilities. We identified three targets of these miRNAs with potential role in tumor aggressiveness: murine double minute 2, thymidylate synthase, and Smad Interacting protein 1/zinc finger E-box binding homeobox 2. We observed a convergent effect (the same molecule can be targeted by all three miRNAs) and a divergent effect (the same miRNA can control multiple targets) for these miRNAs. We experimentally validated these miRNA-target interactions using three independent approaches. First, we observed that miRNA overexpression significantly reduces the mRNA and protein levels of their targets. In the second, we observed significant reduction of the luciferase signal of a vector containing the 3'UTR of the target upon miRNA overexpression. Finally, we show the presence of inverse correlation between miRNA changes and the expression levels of their targets in patient specimens. We also examined the prognostic significance of miR-215 in renal cell carcinoma. Lower expression of miR-215 is associated with significantly reduced disease-free survival time. These findings were validated on an independent data set from The Cancer Genome Atlas. These results can pave the way to the clinical use of miRNAs as prognostic markers and therapeutic targets.

Circulating miR-1, miR-133a, and miR-206 levels are increased after a half-marathon run.

PubMed

Gomes, Clarissa P C; Oliveira, Getúlio P; Madrid, Bibiano; Almeida, Jeeser A; Franco, Octávio L; Pereira, Rinaldo W

2014-11-01

Circulating miRNAs are potential biomarkers that can be important molecules driving cell-to-cell communication. To investigate circulating muscle-specific miRNAs in recreational athletes. Three miRNAs from whole plasma before and after a half-marathon were analyzed by qPCR. MiR-1, -133a, and -206 significantly increased after the race. Increased levels of miRNAs after exercise point to potential biomarkers and to the possibility of being functional players following endurance training. These miRNAs are potential biomarkers of muscle damage or adaptation to exercise.

miR-148a- and miR-216a-regulated oncolytic adenoviruses targeting pancreatic tumors attenuate tissue damage without perturbation of miRNA activity.

PubMed

Bofill-De Ros, Xavier; Gironella, Meritxell; Fillat, Cristina

2014-09-01

Oncolytic virotherapy shows promise for pancreatic ductal adenocarcinoma (PDAC) treatment, but there is the need to minimize associated-toxicities. In the current work, we engineered artificial target sites recognized by miR-216a and/or miR-148a to provide pancreatic tumor-selectivity to replication-competent adenoviruses (Ad-miRTs) and improve their safety profile. Expression analysis in PDAC patients identified miR-148a and miR-216a downregulated in resectable (FC(miR-148a) = 0.044, P < 0.05; FC(miR-216a) = 0.017, P < 0.05), locally advanced (FC(miR-148a) = 0.038, P < 0.001; FC(miR-216a) = 0.001, P < 0.001) and metastatic tumors (FC(miR-148a) = 0.041, P < 0.01; FC(miR-216a) = 0.002, P < 0.001). In mouse tissues, miR-216a was highly specific of the exocrine pancreas whereas miR-148a was abundant in the exocrine pancreas, Langerhans islets, and the liver. In line with the miRNA content and the miRNA target site design, we show E1A gene expression and viral propagation efficiently controlled in Ad-miRT-infected cells. Consequently, Ad-miRT-infected mice presented reduced pancreatic and liver damage without perturbation of the endogenous miRNAs and their targets. Interestingly, the 8-miR148aT design showed repressing activity by all miR-148/152 family members with significant detargeting effects in the pancreas and liver. Ad-miRTs preserved their oncolytic activity and triggered strong antitumoral responses. This study provides preclinical evidences of miR-148a and miR-216a target site insertions to confer adenoviral selectivity and proposes 8-miR148aT as an optimal detargeting strategy for genetically-engineered therapies against PDAC.

287. Dennis Hill, Photographer July 1998 DETAIL VIEW OF UPPER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

287. Dennis Hill, Photographer July 1998 DETAIL VIEW OF UPPER CHORD OF THROUGH TRUSS AT PANEL POINT, FACING NORTHWEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

IVHS Institutional and Legal Issues Program: Review of the FAST-TRAC Operational Test

DOT National Transportation Integrated Search

1994-06-01

The FAST-TRAC (Faster and Safer Travel through Traffic Routing and Advanced Controls) operational test attempted to integrate advanced traveler information systems (ATIS) and advanced traffic management system (ATMS) technologies in Oakland County, M...

miR-200a/miR-141 and miR-205 upregulation might be associated with hormone receptor status and prognosis in endometrial carcinomas.

PubMed

Dong, Ying; Si, Jing-Wen; Li, Wen-Ting; Liang, Li; Zhao, Jian; Zhou, Mei; Li, Dong; Li, Ting

2015-01-01

The aim of this study was to compare the clinicopathological significance of miR-200a/miR-141 and miR-205 expression in endometrioid carcinomas (ECs) versus nonendometrioid carcinomas (NECs) and to assess their correlation with hormone receptor status. miR-200a/miR-141 and miR-205 expression in 154 endometrial cancers was determined by qRT-PCR. The status of estrogen and progesterone receptor (ER/PR) was assessed using immunohistochemistry. miR-200a/miR-141 and miR-205 increased significantly in ECs and in NECs. The expression level of miR-200a was significantly higher in NECs than in ECs (P=0.025). Furthermore, there was a trend that NECs with worse clinicopathological variables had a higher miR-200a expression, while an inverse trend existed in ECs. miR-205 upregulation occurred frequently in NECs without lymph node metastases (P=0.030), whereas such association was not present in ECs. Interestingly, In ECs, miR-200a/miR-141 upregulation occurred frequently in the hormone receptor positive subgroups than the negative subgroups (P<0.05). Similarly, the expression level of miR-205 was higher in the hormone receptor positive subgroups and the association between miR-205 and PR reached statistical significance (P=0.024). In contrast, in NECs, a negative correlation was found between miR-200a/miR-141 and ER or PR status. Meanwhile, in ECs, miR-200a upregulation correlated with prolonged survival in the ER positive subgroup (P=0.046), whereas an inverse trend existed in the ER negative subgroup. Our findings suggest that miR-200a/miR-141 and miR-205 increased significantly in ECs and in NECs. However, they might behave differently in ECs versus NECs. miR-200a/miR-141 and miR-205 might be associated with hormone receptor status in endometrial cancer and may possess prognostic impacts.

miRNAtools: Advanced Training Using the miRNA Web of Knowledge.

PubMed

Stępień, Ewa Ł; Costa, Marina C; Enguita, Francisco J

2018-02-16

Micro-RNAs (miRNAs) are small non-coding RNAs that act as negative regulators of the genomic output. Their intrinsic importance within cell biology and human disease is well known. Their mechanism of action based on the base pairing binding to their cognate targets have helped the development not only of many computer applications for the prediction of miRNA target recognition but also of specific applications for functional assessment and analysis. Learning about miRNA function requires practical training in the use of specific computer and web-based applications that are complementary to wet-lab studies. In order to guide the learning process about miRNAs, we have created miRNAtools (http://mirnatools.eu), a web repository of miRNA tools and tutorials. This article compiles tools with which miRNAs and their regulatory action can be analyzed and that function to collect and organize information dispersed on the web. The miRNAtools website contains a collection of tutorials that can be used by students and tutors engaged in advanced training courses. The tutorials engage in analyses of the functions of selected miRNAs, starting with their nomenclature and genomic localization and finishing with their involvement in specific cellular functions.

Regulation of miRNA Processing and miRNA Mediated Gene Repression in Cancer

PubMed Central

Bajan, Sarah; Hutvagner, Gyorgy

2014-01-01

The majority of human protein-coding genes are predicted to be targets of miRNA-mediated post-transcriptional regulation. The widespread influence of miRNAs is illustrated by their essential roles in all biological processes. Regulated miRNA expression is essential for maintaining cellular differentiation; therefore alterations in miRNA expression patterns are associated with several diseases, including various cancers. High-throughput sequencing technologies revealed low level expressing miRNA isoforms, termed isomiRs. IsomiRs may differ in sequence, length, target preference and expression patterns from their parental miRNA and can arise from differences in miRNA biosynthesis, RNA editing, or SNPs inherent to the miRNA gene. The association between isomiR expression and disease progression is largely unknown. Misregulated miRNA expression is thought to contribute to the formation and/or progression of cancer. However, due to the diversity of targeted transcripts, miRNAs can function as both tumor-suppressor genes and oncogenes as defined by cellular context. Despite this, miRNA profiling studies concluded that the differential expression of particular miRNAs in diseased tissue could aid the diagnosis and treatment of some cancers. PMID:25069508

188. Frank Deras Jr., Photographer April 1998 VIEW OF SUSPENSION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

188. Frank Deras Jr., Photographer April 1998 VIEW OF SUSPENSION BRIDGE, SOUTH SIDE, WITH SAN FRANCISCO IN BACKGROUND, FACING WEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

DISTANT VIEW OF ST. FRANCIS DE SALES CATHEDRAL, LOOKING NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DISTANT VIEW OF ST. FRANCIS DE SALES CATHEDRAL, LOOKING NORTH ALONG MARTIN LUTHER KING JR. WAY FROM 14TH STREET - St. Francis de Sales Church, 2100 Martin Luther King, Jr. Way, Oakland, Alameda County, CA

Privatization and regulation of transport infrastructure in the 1990s : successes ... and bugs to fix for the next millennium

DOT National Transportation Integrated Search

1999-11-01

The FAST-TRAC (Faster and Safer Travel through Traffic Routing and Advanced Controls) Operational Field Test (OFT) is an Intelligent Transportation Systems (ITS) project being conducted in Southeast Michigan, largely within Oakland County. The projec...

Clinical impact of circulating miR-133, miR-1291 and miR-663b in plasma of patients with acute myocardial infarction.

PubMed

Peng, Liu; Chun-guang, Qiu; Bei-fang, Li; Xue-zhi, Ding; Zi-hao, Wang; Yun-fu, Li; Yan-ping, Dang; Yang-gui, Liu; Wei-guo, Li; Tian-yong, Hu; Zhen-wen, Huang

2014-05-01

Acute myocardial infarction (AMI) is one of the leading causes for death in both developed and developing countries and it is the single largest cause of death in the United States, responsible for 1 out of every 6 deaths. The objective of this study was to determine microRNA (miRNA) expression in AMI and determine whether miR-133, miR-1291 and miR-663b could be measured in plasma as a biomarker for recurrence. Patients with AMI and those without AMI were retrospectively recruited for a comparison of their plasma miR-133, miR-1291 and miR-663b expression. miR-133, miR-1291 and miR-663b levels were significantly overexpressed in AMI compared with Non-AMI. MiR-133 showed an AUC of 0.912, with a sensitivity of 81.1% and a specificity of 91.2%. The AUC for miR-1291 was 0.695, with a sensitivity of 78.4% and a specificity of 89.5%. The AUC for miR-663b was 0.611, with a sensitivity of 72.4% and a specificity of 76.5%. This study demonstrated that the levels of miR-133, miR-1291 and miR-663b are associated with AMI. The potential of these miRNAs as biomarkers to improve patient stratification according to the risk of AMI and as circulating biomarkers for the AMI progonos warrants further study. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/8183629061241474.

+2 Valence Metal Concentrations in Lion Creek, Oakland, California

NASA Astrophysics Data System (ADS)

Vazquez, P.; Zedd, T.; Chagolla, R.; Dutton-Starbuck, M.; Negrete, A.; Jinham, M.; Lapota, M.

2012-12-01

Seven major creeks exist within the City of Oakland, California. These creeks all flow in the southwest direction from forested hills down through densely populated streets where they become susceptible to urban runoff. Lion Creek has been diverted to engineered channels and underground culverts and runs directly under our school (Roots International) before flowing into the San Leandro Bay. One branch of the creek begins near an abandoned sulfur mine. Previous studies have shown that extremely high levels of lead, arsenic and iron exist in this portion of the creek due to acid mine drainage. In this study +2 valence heavy metals concentration data was obtained from samples collected from a segment of the creek located approximately 2.8 miles downstream from the mine. Concentrations in samples collected at three different sites along this segment ranged between 50 ppb and 100 ppb. We hypothesize that these levels are related to the high concentration of +2 valence heavy metals at the mining site. To test this hypothesis, we have obtained samples from various locations along the roughly 3.75 miles of Lion Creek that are used to assess changes in heavy metals concentration levels from the mining site to the San Leandro Bay.

Altered expression of four miRNA (miR-1238-3p, miR-202-3p, miR-630 and miR-766-3p) and their potential targets in peripheral blood from vitiligo patients.

PubMed

Shang, Zhiwei; Li, Hongwen

2017-10-01

Vitiligo is an acquired skin disease with pigmentary disorder. Autoimmune destruction of melanocytes is thought to be major factor in the etiology of vitiligo. miRNA-based regulators of gene expression have been reported to play crucial roles in autoimmune disease. Therefore, we attempt to profile the miRNA expressions and predict their potential targets, assessing the biological functions of differentially expressed miRNA. Total RNA was extracted from peripheral blood of vitiligo (experimental group, n = 5) and non-vitiligo (control group, n = 5) age-matched patients. Samples were hybridized to a miRNA array. Box, scatter and principal component analysis plots were performed, followed by unsupervised hierarchical clustering analysis to classify the samples. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was conducted for validation of microarray data. Three different databases, TargetScan, PITA and microRNA.org, were used to predict the potential target genes. Gene ontology (GO) annotation and pathway analysis were performed to assess the potential functions of predicted genes of identified miRNA. A total of 100 (29 upregulated and 71 downregulated) miRNA were filtered by volcano plot analysis. Four miRNA were validated by quantitative RT-PCR as significantly downregulated in the vitiligo group. The functions of predicted target genes associated with differentially expressed miRNA were assessed by GO analysis, showing that the GO term with most significantly enriched target genes was axon guidance, and that the axon guidance pathway was most significantly correlated with these miRNA. In conclusion, we identified four downregulated miRNA in vitiligo and assessed the potential functions of target genes related to these differentially expressed miRNA. © 2017 Japanese Dermatological Association.

1. Photocopy of painting (original painting in possesion of the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Photocopy of painting (original painting in possesion of the family of Judge Jasper Brinton, Cairo, Egypt) Thomas Eakins, Artist 1878 SIDE AND FRONT ELEVATIONS - Brinton 1704 House, Oakland Road (Birmingham Township), Dilworthtown, Chester County, PA

345. Caltrans, Photographer September 20, 1935 "WEST ANCHOR ARM"; DETAIL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

345. Caltrans, Photographer September 20, 1935 "WEST ANCHOR ARM"; DETAIL VIEW OF CANTILEVER TRUSS WEST ANCHOR ARM UNDER CONSTRUCTION. 7-1023 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF CANTILEVER TRUSS CANTILEVER ARM AND SUSPENDED SPAN, NORTH SIDE, FACING SOUTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

MiMiR--an integrated platform for microarray data sharing, mining and analysis.

PubMed

Tomlinson, Chris; Thimma, Manjula; Alexandrakis, Stelios; Castillo, Tito; Dennis, Jayne L; Brooks, Anthony; Bradley, Thomas; Turnbull, Carly; Blaveri, Ekaterini; Barton, Geraint; Chiba, Norie; Maratou, Klio; Soutter, Pat; Aitman, Tim; Game, Laurence

2008-09-18

Despite considerable efforts within the microarray community for standardising data format, content and description, microarray technologies present major challenges in managing, sharing, analysing and re-using the large amount of data generated locally or internationally. Additionally, it is recognised that inconsistent and low quality experimental annotation in public data repositories significantly compromises the re-use of microarray data for meta-analysis. MiMiR, the Microarray data Mining Resource was designed to tackle some of these limitations and challenges. Here we present new software components and enhancements to the original infrastructure that increase accessibility, utility and opportunities for large scale mining of experimental and clinical data. A user friendly Online Annotation Tool allows researchers to submit detailed experimental information via the web at the time of data generation rather than at the time of publication. This ensures the easy access and high accuracy of meta-data collected. Experiments are programmatically built in the MiMiR database from the submitted information and details are systematically curated and further annotated by a team of trained annotators using a new Curation and Annotation Tool. Clinical information can be annotated and coded with a clinical Data Mapping Tool within an appropriate ethical framework. Users can visualise experimental annotation, assess data quality, download and share data via a web-based experiment browser called MiMiR Online. All requests to access data in MiMiR are routed through a sophisticated middleware security layer thereby allowing secure data access and sharing amongst MiMiR registered users prior to publication. Data in MiMiR can be mined and analysed using the integrated EMAAS open source analysis web portal or via export of data and meta-data into Rosetta Resolver data analysis package. The new MiMiR suite of software enables systematic and effective capture of extensive

A path-based measurement for human miRNA functional similarities using miRNA-disease associations

NASA Astrophysics Data System (ADS)

Ding, Pingjian; Luo, Jiawei; Xiao, Qiu; Chen, Xiangtao

2016-09-01

Compared with the sequence and expression similarity, miRNA functional similarity is so important for biology researches and many applications such as miRNA clustering, miRNA function prediction, miRNA synergism identification and disease miRNA prioritization. However, the existing methods always utilized the predicted miRNA target which has high false positive and false negative to calculate the miRNA functional similarity. Meanwhile, it is difficult to achieve high reliability of miRNA functional similarity with miRNA-disease associations. Therefore, it is increasingly needed to improve the measurement of miRNA functional similarity. In this study, we develop a novel path-based calculation method of miRNA functional similarity based on miRNA-disease associations, called MFSP. Compared with other methods, our method obtains higher average functional similarity of intra-family and intra-cluster selected groups. Meanwhile, the lower average functional similarity of inter-family and inter-cluster miRNA pair is obtained. In addition, the smaller p-value is achieved, while applying Wilcoxon rank-sum test and Kruskal-Wallis test to different miRNA groups. The relationship between miRNA functional similarity and other information sources is exhibited. Furthermore, the constructed miRNA functional network based on MFSP is a scale-free and small-world network. Moreover, the higher AUC for miRNA-disease prediction indicates the ability of MFSP uncovering miRNA functional similarity.

133. Dennis Hill, Photographer January 1998 VIEW OF TRANSBAY TERMINAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

133. Dennis Hill, Photographer January 1998 VIEW OF TRANSBAY TERMINAL BUS LOOP FROM HOWARD STREET BETWEEN FIRST AND SECOND STREETS, FACING EAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ANCHOR ARM AT PIERS E- AND E-2, SOUTH SIDE, FACING NORTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

215. Dennis Hill, Photographer May 1998 DETAIL VIEW OF STRAND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

215. Dennis Hill, Photographer May 1998 DETAIL VIEW OF STRAND SHOES AND STORM CABLE EYE BARS IN YERBA BUENA ANCHORAGE, FACING EAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

miR-181a and miR-630 regulate cisplatin-induced cancer cell death.

PubMed

Galluzzi, Lorenzo; Morselli, Eugenia; Vitale, Ilio; Kepp, Oliver; Senovilla, Laura; Criollo, Alfredo; Servant, Nicolas; Paccard, Caroline; Hupé, Philippe; Robert, Thomas; Ripoche, Hugues; Lazar, Vladimir; Harel-Bellan, Annick; Dessen, Philippe; Barillot, Emmanuel; Kroemer, Guido

2010-03-01

MicroRNAs (miRNA) are noncoding RNAs that regulate multiple cellular processes, including proliferation and apoptosis. We used microarray technology to identify miRNAs that were upregulated by non-small cell lung cancer (NSCLC) A549 cells in response to cisplatin (CDDP). The corresponding synthetic miRNA precursors (pre-miRNAs) per se were not lethal when transfected into A549 cells yet affected cell death induction by CDDP, C2-ceramide, cadmium, etoposide, and mitoxantrone in an inducer-specific fashion. Whereas synthetic miRNA inhibitors (anti-miRNAs) targeting miR-181a and miR-630 failed to modulate the response of A549 to CDDP, pre-miR-181a and pre-miR-630 enhanced and reduced CDDP-triggered cell death, respectively. Pre-miR-181a and pre-miR-630 consistently modulated mitochondrial/postmitochondrial steps of the intrinsic pathway of apoptosis, including Bax oligomerization, mitochondrial transmembrane potential dissipation, and the proteolytic maturation of caspase-9 and caspase-3. In addition, pre-miR-630 blocked early manifestations of the DNA damage response, including the phosphorylation of the ataxia-telangiectasia mutated (ATM) kinase and of two ATM substrates, histone H2AX and p53. Pharmacologic and genetic inhibition of p53 corroborated the hypothesis that pre-miR-630 (but not pre-miR-181a) blocks the upstream signaling pathways that are ignited by DNA damage and converge on p53 activation. Pre-miR-630 arrested A549 cells in the G0-G1 phase of the cell cycle, correlating with increased levels of the cell cycle inhibitor p27(Kip1) as well as with reduced proliferation rates and resulting in greatly diminished sensitivity of A549 cells to the late S-G2-M cell cycle arrest mediated by CDDP. Altogether, these results identify miR-181a and miR-630 as novel modulators of the CDDP response in NSCLC.

Decreased miR-128 and increased miR-21 synergistically cause podocyte injury in sepsis.

PubMed

Wang, Shanshan; Wang, Jun; Zhang, Zengdi; Miao, Hongjun

2017-08-01

Glomerular podocytes are injured in sepsis. We studied, in a sepsis patient, whether microRNAs (miRNAs) play a role in the podocyte injury. Podocytes were cultured and treated with lipopolysaccharide (LPS). Filtration barrier function of podocyte was analyzed with albumin influx assay. Nephrin level was analyzed with reverse transcription polymerase chain reaction (RT-PCR) and western blot. MiRNAs were detected using miRNAs PCR Array and in situ hybridization. MiRNA target sites were evaluated with luciferase reporter assays. LPS impaired the filtration barrier function of podocytes. MiR-128 level was decreased and miR-21 level was increased in podocytes in vitro and in the sepsis patient. The decrease in miR-128 was sufficient to induce the loss of nephrin and the impairment of filtration barrier function, while the increase of miR-21 exacerbated the process. Snail and phosphatase and tensin homolog (PTEN) were identified as the targets of miR-128 and miR-21. Decreased miR-128 induced Snail expression, and the increased miR-21 stabilized Snail by regulating the PTEN/Akt/GSK3β pathway. Supplementation of miR-128 and inhibition of miR-21 suppressed Snail expression and prevented the podocyte injury induced by LPS. Our study suggests that decreased miR-128 and increased miR-21 synergistically cause podocyte injury and are the potential therapeutic targets in sepsis.

Identification of direct target genes of miR-7, miR-9, miR-96, and miR-182 in the human breast cancer cell lines MCF-7 and MDA-MB-231.

PubMed

Moazzeni, Hamidreza; Najafi, Ali; Khani, Marzieh

2017-08-01

Some microRNAs have carcinogenic or tumor suppressive effects in breast cancer, which is the most common cancer in women worldwide. MiR-7 and miR-9 are tumor suppressor microRNAs, which induce apoptosis and inhibit proliferation in breast cancer cells. Moreover, miR-96 and miR-182 are onco-microRNAs that increase proliferation, migration, and tumorigenesis in breast cancer cells. This study aimed to identify the direct target genes of these four microRNAs in the human breast cancer cell lines MCF-7 and MDA-MB-231. Initially, bioinformatics tools were used to identify the target genes that have binding sites for miR-7, MiR-9, MiR-96, and miR-182 and are also associated with breast cancer. Subsequently, the findings of the bioinformatics analysis relating to the effects of these four microRNAs on the 3'-UTR activity of the potential target genes were confirmed using the dual luciferase assay in MCF-7 and MDA-MB-231 cells co-transfected with the vectors containing 3'-UTR segments of the target genes downstream of a luciferase coding gene and each of the microRNAs. Finally, the effects of microRNAs on the endogenous expression of potential target genes were assessed by the overexpression of each of the four microRNAs in MCF-7 and MDA-MB-231 cells. Respectively, three, three, three, and seven genes were found to have binding sites for miR-7, miR-9, miR-96, and miR-182 and were associated with breast cancer. The results of empirical studies including dual luciferase assays and real-time PCR confirmed that miR-7 regulates the expression of BRCA1 and LASP1; MiR-9 regulates the expression of AR; miR-96 regulates the expression of ABCA1; and miR-182 regulates the expression of NBN, TOX3, and LASP1. Taken together, our results suggest that the tumor suppressive effects of miR-7 may be mediated partly by regulating the expression of BRCA1 as a tumor suppressor gene in breast cancer. In addition, this microRNA and miR-182 may have effects on the nodal-positivity and tumor

Community-Based Participatory Research and Policy Advocacy to Reduce Diesel Exposure in West Oakland, California

PubMed Central

Gonzalez, Priscilla A.; Garcia, Analilia P.; Gordon, Margaret; Garzón, Catalina; Palaniappan, Meena; Prakash, Swati; Beveridge, Brian

2011-01-01

We conducted a multimethod case study analysis of a community-based participatory research partnership in West Oakland, California, and its efforts to study and address the neighborhood's disproportionate exposure to diesel air pollution. We employed 10 interviews with partners and policymakers, participant observation, and a review of documents. Results of the partnership's truck count and truck idling studies suggested substantial exposure to diesel pollution and were used by the partners and their allies to make the case for a truck route ordinance. Despite weak enforcement, the partnership's increased political visibility helped change the policy environment, with the community partner now heavily engaged in environmental decision-making on the local and regional levels. Finally, we discussed implications for research, policy, and practice. PMID:21551381

Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development

PubMed Central

Granados-López, Angelica Judith; Ruiz-Carrillo, José Luis; Servín-González, Luis Steven; Martínez-Rodríguez, José Luis; Reyes-Estrada, Claudia Araceli; Gutiérrez-Hernández, Rosalinda; López, Jesús Adrián

2017-01-01

Aberrant miRNA expression is well recognized as a cancer hallmark, nevertheless miRNA function and expression does not always correlate in patients tissues and cell lines studies. In addition to this issue, miRNA strand usage conduces to increased cell signaling pathways modulation diversifying cellular processes regulation. In cervical cancer, 20 miRNA families are involved in carcinogenesis induction and development to this moment. These families have 5p and 3p strands with different nucleotide (nt) chain sizes. In general, mature 5p strands are larger: two miRNAs of 24 nt, 24 miRNAs of 23 nt, 35 miRNAs of 22 nt and three miRNAs of 21 nt. On the other hand, the 3p strands lengths observed are: seven miRNAs of 23 nt, 50 miRNAs of 22 nt, six miRNAs of 21 nt and four miRNAs of 20 nt. Based on the analysis of the 20 miRNA families associated with cervical cancer, 67 3p strands and 65 5p strands are selected suggesting selectivity and specificity mechanisms regulating cell processes like proliferation, apoptosis, migration, invasion, metabolism and Warburg effect. The insight reviewed here could be used in the miRNA based therapy, diagnosis and prognosis approaches. PMID:28216603

Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development.

PubMed

Granados-López, Angelica Judith; Ruiz-Carrillo, José Luis; Servín-González, Luis Steven; Martínez-Rodríguez, José Luis; Reyes-Estrada, Claudia Araceli; Gutiérrez-Hernández, Rosalinda; López, Jesús Adrián

2017-02-14

Aberrant miRNA expression is well recognized as a cancer hallmark, nevertheless miRNA function and expression does not always correlate in patients tissues and cell lines studies. In addition to this issue, miRNA strand usage conduces to increased cell signaling pathways modulation diversifying cellular processes regulation. In cervical cancer, 20 miRNA families are involved in carcinogenesis induction and development to this moment. These families have 5p and 3p strands with different nucleotide (nt) chain sizes. In general, mature 5p strands are larger: two miRNAs of 24 nt, 24 miRNAs of 23 nt, 35 miRNAs of 22 nt and three miRNAs of 21 nt. On the other hand, the 3p strands lengths observed are: seven miRNAs of 23 nt, 50 miRNAs of 22 nt, six miRNAs of 21 nt and four miRNAs of 20 nt. Based on the analysis of the 20 miRNA families associated with cervical cancer, 67 3p strands and 65 5p strands are selected suggesting selectivity and specificity mechanisms regulating cell processes like proliferation, apoptosis, migration, invasion, metabolism and Warburg effect. The insight reviewed here could be used in the miRNA based therapy, diagnosis and prognosis approaches.

Effects of diesel particle filter retrofits and accelerated fleet turnover on drayage truck emissions at the Port of Oakland.

PubMed

Dallmann, Timothy R; Harley, Robert A; Kirchstetter, Thomas W

2011-12-15

Heavy-duty diesel drayage trucks have a disproportionate impact on the air quality of communities surrounding major freight-handling facilities. In an attempt to mitigate this impact, the state of California has mandated new emission control requirements for drayage trucks accessing ports and rail yards in the state beginning in 2010. This control rule prompted an accelerated diesel particle filter (DPF) retrofit and truck replacement program at the Port of Oakland. The impact of this program was evaluated by measuring emission factor distributions for diesel trucks operating at the Port of Oakland prior to and following the implementation of the emission control rule. Emission factors for black carbon (BC) and oxides of nitrogen (NO(x)) were quantified in terms of grams of pollutant emitted per kilogram of fuel burned using a carbon balance method. Concentrations of these species along with carbon dioxide were measured in the exhaust plumes of individual diesel trucks as they drove by en route to the Port. A comparison of emissions measured before and after the implementation of the truck retrofit/replacement rule shows a 54 ± 11% reduction in the fleet-average BC emission factor, accompanied by a shift to a more highly skewed emission factor distribution. Although only particulate matter mass reductions were required in the first year of the program, a significant reduction in the fleet-average NO(x) emission factor (41 ± 5%) was observed, most likely due to the replacement of older trucks with new ones.

An Investigation of Air Quality Surrounding Lake Merritt in Oakland, California

NASA Astrophysics Data System (ADS)

Ararso, I.; Casino, N.; Chen, B.; Johnson, J.; Koerber, K. W.; Lau, S.; Truisi, V.; Yanez, M.; Yeung, A.; Unigarro, M.; Vue, G.; Garduno, L.; Cuff, K.

2005-12-01

Lake Merritt is a naturally occurring inlet from the San Francisco Bay that was converted into an urban lake near downtown Oakland in 1860. The Lake itself is located within the Lake Merritt Park and Wildlife Refuge, home to over 90 species of migrating waterfowl, as well as a variety of aquatic wildlife. Its close proximity to downtown, several busy roads, and two major highways makes Lake Merritt a popular destination that is easily accessible to Oakland residents, but also puts it at risk for impaired air quality due to automobile exhaust. In an effort to assess air quality near Lake Merritt, we measured percent oxygen and carbon dioxide (CO2) concentrations in ambient air. These two gases can be used to assess air quality because the significant build up of CO2, which primarily results from the incomplete combustion of personal automobile engines, can result in the reduction of oxygen to concentration levels that are hazardous to human and other life. During the Summer of 2005, air samples from over 90 different locations were collected and used to make these measurements. Measurements were made with PASCO data-loggers attached to sensors that use infrared detectors to measure the amount of energy absorbed by carbon dioxide and oxygen molecules. Results were statistically analyzed, mapped, and used to assess the overall quality of air surrounding the Lake. Preliminary analysis of oxygen data indicates that higher concentration levels occur near sections of the Lake that are furthest removed from major roads, as well as in areas that have significant amounts of vegetation. In fact, the highest value recorded in this study was measured in a sample obtained near a grove of trees in a portion of the park that has the most vegetation, and that is furthest removed from major roads. Air quality here is high primarily due to the absence of CO2 build up associated with automobile traffic. The lowest values recorded were measured in samples collected along a stretch of

Analysis of corridor delay under SCATS control : FAST-TRAC Phase III deliverable

DOT National Transportation Integrated Search

1998-04-01

The study was designed to determine the change in travel time following the implementation of the Sydney Coordinated Adaptive Traffic System (SCATS) in Oakland County, Michigan. A before/after comparison was used to examine the change in travel time ...

348. Caltrans, Photographer December 27, 1935 "TOWER E3"; VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

348. Caltrans, Photographer December 27, 1935 "TOWER E-3"; VIEW OF TOWER E-3 AND CANTILEVER TRUSS EAST ANCHOR ARM UNDER CONSTRUCTION. 7-1128 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

MiRduplexSVM: A High-Performing MiRNA-Duplex Prediction and Evaluation Methodology

PubMed Central

Karathanasis, Nestoras; Tsamardinos, Ioannis; Poirazi, Panayiota

2015-01-01

We address the problem of predicting the position of a miRNA duplex on a microRNA hairpin via the development and application of a novel SVM-based methodology. Our method combines a unique problem representation and an unbiased optimization protocol to learn from mirBase19.0 an accurate predictive model, termed MiRduplexSVM. This is the first model that provides precise information about all four ends of the miRNA duplex. We show that (a) our method outperforms four state-of-the-art tools, namely MaturePred, MiRPara, MatureBayes, MiRdup as well as a Simple Geometric Locator when applied on the same training datasets employed for each tool and evaluated on a common blind test set. (b) In all comparisons, MiRduplexSVM shows superior performance, achieving up to a 60% increase in prediction accuracy for mammalian hairpins and can generalize very well on plant hairpins, without any special optimization. (c) The tool has a number of important applications such as the ability to accurately predict the miRNA or the miRNA*, given the opposite strand of a duplex. Its performance on this task is superior to the 2nts overhang rule commonly used in computational studies and similar to that of a comparative genomic approach, without the need for prior knowledge or the complexity of performing multiple alignments. Finally, it is able to evaluate novel, potential miRNAs found either computationally or experimentally. In relation with recent confidence evaluation methods used in miRBase, MiRduplexSVM was successful in identifying high confidence potential miRNAs. PMID:25961860

miRNAs in brain development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Petri, Rebecca; Malmevik, Josephine; Fasching, Liana

2014-02-01

MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs havemore » been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.« less

Cell type-specific deficiency of c-kit gene expression in mutant mice of mi/mi genotype.

PubMed Central

Isozaki, K.; Tsujimura, T.; Nomura, S.; Morii, E.; Koshimizu, U.; Nishimune, Y.; Kitamura, Y.

1994-01-01

The mi locus of mice encodes a novel member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors (hereafter called mi factor). In addition to microphthalmus, osteopetrosis, and lack of melanocytes, mice of mi/mi genotype are deficient in mast cells. Since the c-kit receptor tyrosine kinase plays an important role in the development of mast cells, and since the c-kit expression by cultured mast cells from mi/mi mice is deficient in both mRNA and protein levels, the mast cell deficiency of mi/mi mice has been attributed at least in part to the deficient expression of c-kit. However, it remained to be examined whether the c-kit expression was also deficient in tissues of mi/mi mice. In the present study, we examined the c-kit expression by mi/mi skin mast cells using in situ hybridization and immunohistochemistry. Moreover, we examined the c-kit expression by various cells other than mast cells in tissues of mi/mi mice. We found that the c-kit expression was deficient in mast cells but not in erythroid precursors, testicular germ cells, and neurons of mi/mi mice. This suggested that the regulation of the c-kit transcription by the mi factor was dependent on cell types. Mice of mi/mi genotype appeared to be a useful model to analyze the function of transcription factors in the whole-animal level. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:7524330

VIEW TO NORTHWEST, SHOWING FACILITY NO. 525 AND HOSPITAL (FACILITY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW TO NORTHWEST, SHOWING FACILITY NO. 525 AND HOSPITAL (FACILITY No. 515) BEYOND. See CA-2398-CP-8 for detail of the stairway in the distance - Hamilton Field, Amphitheater, North Oakland Drive near East Hospital Drive, Novato, Marin County, CA

236. Frank Deras Jr., Photographer May 1998 AERIAL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

236. Frank Deras Jr., Photographer May 1998 AERIAL VIEW OF DECK TRUSS AT YERBA BUENA ISLAND, SOUTH SIDE, WITH TREASURE ISLAND IN BACKGROUND, FACING NORTH NORTHWEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

238. Frank Deras Jr., Photographer May 1998 AERIAL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

238. Frank Deras Jr., Photographer May 1998 AERIAL VIEW OF DECK TRUSS AT YERBA BUENA ISLAND, SOUTH SIDE, WITH TREASURE ISLAND IN BACKGROUND, FACING NORTHWEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

346. Caltrans, Photographer July 8, 1935 "PIER El"; VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

346. Caltrans, Photographer July 8, 1935 "PIER E-l"; VIEW OF PIER E-I, DECK TRUSS, AND CANTILEVER TRUSS ANCHOR ARM UNDER CONSTRUCTION. 5-1583 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

213. Frank Deras Jr., Photographer May 1998 DETAIL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

213. Frank Deras Jr., Photographer May 1998 DETAIL VIEW OF ROCKER ARM IN YERBA BUENA ANCHORAGE, NORTHWEST CORNER (HUMAN SCALE: CHUCK HAZELWOOD, CALTRANS EMPLOYEE), FACING WEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

MiMiR – an integrated platform for microarray data sharing, mining and analysis

PubMed Central

Tomlinson, Chris; Thimma, Manjula; Alexandrakis, Stelios; Castillo, Tito; Dennis, Jayne L; Brooks, Anthony; Bradley, Thomas; Turnbull, Carly; Blaveri, Ekaterini; Barton, Geraint; Chiba, Norie; Maratou, Klio; Soutter, Pat; Aitman, Tim; Game, Laurence

2008-01-01

Background Despite considerable efforts within the microarray community for standardising data format, content and description, microarray technologies present major challenges in managing, sharing, analysing and re-using the large amount of data generated locally or internationally. Additionally, it is recognised that inconsistent and low quality experimental annotation in public data repositories significantly compromises the re-use of microarray data for meta-analysis. MiMiR, the Microarray data Mining Resource was designed to tackle some of these limitations and challenges. Here we present new software components and enhancements to the original infrastructure that increase accessibility, utility and opportunities for large scale mining of experimental and clinical data. Results A user friendly Online Annotation Tool allows researchers to submit detailed experimental information via the web at the time of data generation rather than at the time of publication. This ensures the easy access and high accuracy of meta-data collected. Experiments are programmatically built in the MiMiR database from the submitted information and details are systematically curated and further annotated by a team of trained annotators using a new Curation and Annotation Tool. Clinical information can be annotated and coded with a clinical Data Mapping Tool within an appropriate ethical framework. Users can visualise experimental annotation, assess data quality, download and share data via a web-based experiment browser called MiMiR Online. All requests to access data in MiMiR are routed through a sophisticated middleware security layer thereby allowing secure data access and sharing amongst MiMiR registered users prior to publication. Data in MiMiR can be mined and analysed using the integrated EMAAS open source analysis web portal or via export of data and meta-data into Rosetta Resolver data analysis package. Conclusion The new MiMiR suite of software enables systematic and

Evaluation of miR-182/miR-100 Ratio for Diagnosis and Survival Prediction in Bladder Cancer.

PubMed

Chen, Zhanguo; Wu, Lili; Lin, Qi; Shi, Jing; Lin, Xiangyang; Shi, Liang

2016-09-01

Abnormal expression of microRNAs (miRNAs) plays an important role in development of several cancer types, including bladder cancer (BCa). However, the relationship between the ratio of miR-181/miR-100 and the prognosis of BCa has not been studied yet. The aim of this study was to evaluate the expression of miR-182, miR-100 and their clinical significance in BCa. Upregulation of miR-182 and down-regulation of miR-100 were validated in tissue specimens of 134 BCa cases compared with 148 normal bladder epithelia (NBE) specimens  using TaqMan-based real-time reverse transcription quantitative PCR (RT-qPCR). The diagnostic and prognostic evaluation of miR-182, miR-100, and miR-182/miR-100 ratio was also performed. miR-182 was upregulated in BCa and miR-100 was down-regulated in BCa compared with NBE (P < 0.001). The areas under receiver operating characteristic curves (AUCs-ROC) for miR-182 and miR-100 were 0.913 and 0.810, respectively. However, miR-182/miR-100 ratio increased the diagnostic performance, yielding an AUC of 0.981 (97.01% sensitivity and 90.54% specificity). Moreover, miR-182/miR-100 ratio was associated with pT-stage, histological grade, BCa recurrence and carcinoma in situ (P < 0.05 for all). Multivariate Cox regression analysis indicated that miR-182/miR-100 ratio was an independent prognostic factor for overall survival (Hazard ratio: 7.142; 95% CI: 2.106 - 9.891; P < 0.01). Furthermore, Kaplan-Meier curve analysis revealed that high-level of miR-182/miR-100 ratio was significantly correlated with shortened survival time for BCa patients (P < 0.01). The miR-182/miR-100 ratio may serve as a novel promising biomarker for diagnosis and survival prediction in BCa. Further studies are needed to elucidate the role of miR-182/miR-100 ratio as a non‑invasive diagnostic tool for BCa.

MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer

PubMed Central

Eyking, Annette; Reis, Henning; Frank, Magdalena; Gerken, Guido; Schmid, Kurt W.; Cario, Elke

2016-01-01

Mucinous adenocarcinoma (MAC) represents a distinct histopathological entity of colorectal cancer (CRC), which is associated with disease progression and poor prognosis. Here, we found that expression levels of miR-205 and miR-373 were specifically upregulated only in patients with mucinous colon cancers, but not in CRC that lack mucinous components. To investigate the effects of miR-205 and miR-373 on intestinal epithelial cell (IEC) biology by gain- and loss-of-function experiments in a proof-of-concept approach, we chose previously established in-vitro human Caco-2-based models of differentiated, non-invasive (expressing TLR4 wild-type; termed Caco-2[WT]) versus undifferentiated, invasive (expressing TLR4 mutant D299G; termed Caco-2[D299G]) IEC. Enterocyte-like Caco-2[WT] showed low levels of miR-205 and miR-373 expression, while both miRNAs were significantly upregulated in colorectal carcinoma-like Caco-2[D299G], thus resembling the miRNA expression pattern of paired normal versus tumor samples from MAC patients. Using stable transfection, we generated miR-205- or miR-373-expressing and miR-205- or miR-373-inhibiting subclones of these IEC lines. We found that introduction of miR-205 into Caco-2[WT] led to expansion of mucus-secreting goblet cell-like cells, which was associated with induction of KLF4, MUC2 and TGFβ1 expression. Activation of miR-205 in Caco-2[WT] induced chemoresistance, while inhibition of miR-205 in Caco-2[D299G] promoted chemosensitivity. Caco-2[WT] overexpressing miR-373 showed mitotic abnormalities and underwent morphologic changes (loss of epithelial polarity, cytoskeletal reorganization, and junctional disruption) associated with epithelial-mesenchymal transition and progression to inflammation-associated colonic carcinoma, which correlated with induction of phosphorylated STAT3 and N-CADHERIN expression. Functionally, introduction of miR-373 into Caco-2[WT] mediated loss of cell-cell adhesion and increased proliferation and invasion

MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer.

PubMed

Eyking, Annette; Reis, Henning; Frank, Magdalena; Gerken, Guido; Schmid, Kurt W; Cario, Elke

2016-01-01

Mucinous adenocarcinoma (MAC) represents a distinct histopathological entity of colorectal cancer (CRC), which is associated with disease progression and poor prognosis. Here, we found that expression levels of miR-205 and miR-373 were specifically upregulated only in patients with mucinous colon cancers, but not in CRC that lack mucinous components. To investigate the effects of miR-205 and miR-373 on intestinal epithelial cell (IEC) biology by gain- and loss-of-function experiments in a proof-of-concept approach, we chose previously established in-vitro human Caco-2-based models of differentiated, non-invasive (expressing TLR4 wild-type; termed Caco-2[WT]) versus undifferentiated, invasive (expressing TLR4 mutant D299G; termed Caco-2[D299G]) IEC. Enterocyte-like Caco-2[WT] showed low levels of miR-205 and miR-373 expression, while both miRNAs were significantly upregulated in colorectal carcinoma-like Caco-2[D299G], thus resembling the miRNA expression pattern of paired normal versus tumor samples from MAC patients. Using stable transfection, we generated miR-205- or miR-373-expressing and miR-205- or miR-373-inhibiting subclones of these IEC lines. We found that introduction of miR-205 into Caco-2[WT] led to expansion of mucus-secreting goblet cell-like cells, which was associated with induction of KLF4, MUC2 and TGFβ1 expression. Activation of miR-205 in Caco-2[WT] induced chemoresistance, while inhibition of miR-205 in Caco-2[D299G] promoted chemosensitivity. Caco-2[WT] overexpressing miR-373 showed mitotic abnormalities and underwent morphologic changes (loss of epithelial polarity, cytoskeletal reorganization, and junctional disruption) associated with epithelial-mesenchymal transition and progression to inflammation-associated colonic carcinoma, which correlated with induction of phosphorylated STAT3 and N-CADHERIN expression. Functionally, introduction of miR-373 into Caco-2[WT] mediated loss of cell-cell adhesion and increased proliferation and invasion

miRNAFold: a web server for fast miRNA precursor prediction in genomes.

PubMed

Tav, Christophe; Tempel, Sébastien; Poligny, Laurent; Tahi, Fariza

2016-07-08

Computational methods are required for prediction of non-coding RNAs (ncRNAs), which are involved in many biological processes, especially at post-transcriptional level. Among these ncRNAs, miRNAs have been largely studied and biologists need efficient and fast tools for their identification. In particular, ab initio methods are usually required when predicting novel miRNAs. Here we present a web server dedicated for miRNA precursors identification at a large scale in genomes. It is based on an algorithm called miRNAFold that allows predicting miRNA hairpin structures quickly with high sensitivity. miRNAFold is implemented as a web server with an intuitive and user-friendly interface, as well as a standalone version. The web server is freely available at: http://EvryRNA.ibisc.univ-evry.fr/miRNAFold. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Gene silencing efficiency and INF-β induction effects of splicing miRNA 155-based artificial miRNA with pre-miRNA stem-loop structures.

PubMed

Sin, Onsam; Mabiala, Prudence; Liu, Ye; Sun, Ying; Hu, Tao; Liu, Qingzhen; Guo, Deyin

2012-02-01

Artificial microRNA (miRNA) expression vectors have been developed and used for RNA interference. The secondary structure of artificial miRNA is important for RNA interference efficacy. We designed two groups of six artificial splicing miRNA 155-based miRNAs (SM155-based miRNAs) with the same target in the coding region or 3' UTR of a target gene and studied their RNA silencing efficiency and interferon β (IFN-β) induction effects. SM155-based miRNA with a mismatch at the +1 position and a bulge at the +11, +12 positions in a miRNA precursor stem-loop structure showed the highest gene silencing efficiency and lowest IFN-β induction effect (increased IFN-β mRNA level by 10% in both target cases), regardless of the specificity of the target sequence, suggesting that pSM155-based miRNA with this design could be a valuable miRNA expression vector.

Mi-DISCOVERER: A bioinformatics tool for the detection of mi-RNA in human genome.

PubMed

Arshad, Saadia; Mumtaz, Asia; Ahmad, Freed; Liaquat, Sadia; Nadeem, Shahid; Mehboob, Shahid; Afzal, Muhammad

2010-11-27

MicroRNAs (miRNAs) are 22 nucleotides non-coding RNAs that play pivotal regulatory roles in diverse organisms including the humans and are difficult to be identified due to lack of either sequence features or robust algorithms to efficiently identify. Therefore, we made a tool that is Mi-Discoverer for the detection of miRNAs in human genome. The tools used for the development of software are Microsoft Office Access 2003, the JDK version 1.6.0, BioJava version 1.0, and the NetBeans IDE version 6.0. All already made miRNAs softwares were web based; so the advantage of our project was to make a desktop facility to the user for sequence alignment search with already identified miRNAs of human genome present in the database. The user can also insert and update the newly discovered human miRNA in the database. Mi-Discoverer, a bioinformatics tool successfully identifies human miRNAs based on multiple sequence alignment searches. It's a non redundant database containing a large collection of publicly available human miRNAs.

Mi-DISCOVERER: A bioinformatics tool for the detection of mi-RNA in human genome

PubMed Central

Arshad, Saadia; Mumtaz, Asia; Ahmad, Freed; Liaquat, Sadia; Nadeem, Shahid; Mehboob, Shahid; Afzal, Muhammad

2010-01-01

MicroRNAs (miRNAs) are 22 nucleotides non-coding RNAs that play pivotal regulatory roles in diverse organisms including the humans and are difficult to be identified due to lack of either sequence features or robust algorithms to efficiently identify. Therefore, we made a tool that is Mi-Discoverer for the detection of miRNAs in human genome. The tools used for the development of software are Microsoft Office Access 2003, the JDK version 1.6.0, BioJava version 1.0, and the NetBeans IDE version 6.0. All already made miRNAs softwares were web based; so the advantage of our project was to make a desktop facility to the user for sequence alignment search with already identified miRNAs of human genome present in the database. The user can also insert and update the newly discovered human miRNA in the database. Mi-Discoverer, a bioinformatics tool successfully identifies human miRNAs based on multiple sequence alignment searches. It's a non redundant database containing a large collection of publicly available human miRNAs. PMID:21364831

A New Metamodeling Approach for Time-dependent Reliability of Dynamic Systems with Random Parameters Excited by Input Random Processes

DTIC Science & Technology

2014-04-09

Excited by Input Random Processes Igor Baseski1,2, Dorin Drignei3, Zissimos P. Mourelatos1, Monica Majcher1 Oakland University, Rochester MI 48309 1...CONTRACT NUMBER W56HZV-04-2-0001 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Igor Baseski; Dorin Drignei; Zissimos Mourelatos; Monica

Overexpression of miR-484 and miR-744 in Vero cells alters Dengue virus replication

PubMed Central

Castrillón-Betancur, Juan Camilo; Urcuqui-Inchima, Silvio

2017-01-01

BACKGROUND Dengue is considered one of the world’s most important mosquito-borne diseases. MicroRNAs (miRNAs) are small non-coding single-stranded RNAs that play an important role in the regulation of gene expression in eukaryotes. Although miRNAs possess antiviral activity against many mammalian-infecting viruses, their involvement in Dengue virus (DENV) replication remains poorly understood. OBJECTIVE To determine the role of miR-484 and miR-744 in DENV infection and to examine whether DENV infection alters the expression of both miRNAs. METHODS We used bioinformatics tools to explore the relationship between DENV and cellular miRNAs. We then overexpressed miR-484 or miR-744 in Vero cells to examine their role in DENV replication using flow cytometry, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR), and western blotting. FINDINGS We found several cellular miRNAs that target a conserved region within the 3′ untranslated region (3′ UTR) of the genome of the four DENV serotypes and found that overexpression of miR-484 or miR-744 inhibits infection by DENV-1 to DENV-4. Furthermore, we observed that DENV RNA might be involved in the downregulation of endogenous miR-484 and miR-744. CONCLUSION Our study identifies miR-484 and miR-744 as two possible restriction host factors against DENV infection. However, further studies are needed to directly verify whether miR-484 and miR-744 both have an anti-DENV effect in vivo. PMID:28327787

miR-23a, miR-146a and miR-301a confer predisposition to Vogt-Koyanagi-Harada syndrome but not to Behcet's disease.

PubMed

Hou, Shengping; Ye, Zi; Liao, Dan; Bai, Lin; Liu, Yunjia; Zhang, Jun; Kijlstra, Aize; Yang, Peizeng

2016-01-28

Ninety-eight miRNAs are involved in the immune response. However, the genetic roles of these miRNAs remain unclear in Behcet's disease (BD) and Vogt-Koyanagi-Harada (VKH) syndrome. This study aimed to explore the association and functional roles of copy number variants (CNV) in several miRNAs with BD and VKH syndrome. Genotyping of CNVs was examined by TaqMan PCR. The expression of miR-23a, transfection efficiency and cytokine production were measured by real-time PCR, flow cytometry or ELISA. First, replication and combined studies for miR-23a, miR-146a and miR-301a demonstrated a similar association with VKH syndrome (Combined: P = 5.53 × 10(-8); P = 8.43 × 10(-31); P = 9.23 × 10(-8), respectively). No association of CNVs of the above mentioned miRNAs was observed in BD patients. mRNA expression of miR-23a showed a positive association with its copy numbers. Additionally, individuals with high copy number of miR-23a show an increased production of interleukin-6 (IL-6), but not IL-8 and monocyte chemoattractant protein-1 (MCP-1) by stimulated PBMCs. miR-23a transfected ARPE-19 cells modulated the production of IL-6 and IL-8, but not MCP-1. Our results suggest that CNVs of miR-146a, miR-23a and miR-301a confer susceptibility to VKH syndrome, but not to BD. The contribution of miR-23a to VKH syndrome may be mediated by increasing the production of IL-6.

Hydrostatic pressure as epigenetic modulator in chondrocyte cultures: A study on miRNA-155, miRNA-181a and miRNA-223 expression levels.

PubMed

De Palma, Anna; Cheleschi, Sara; Pascarelli, Nicola Antonio; Giannotti, Stefano; Galeazzi, Mauro; Fioravanti, Antonella

2018-01-03

Mechanical stimuli and hydrostatic pressure (HP) play an important role in the regulation of chondrocytes metabolism. Growing evidence demonstrated the ability of mechanical loading to modulate the expression of microRNA (miRNA) involved in chondrocytes homeostasis and in the pathogenesis of osteoarthritis (OA). The expression of miR-155, miR-181a and miR-223 in normal and OA chondrocyte cultures, and their potential modifications following exposure to three hours of a cyclic HP (1-5 MPa, frequency 0.25 Hz) were investigated. Also evaluated the expression of Chuk, regulator of the NF-kB pathway activation, which is a target gene of miR-223, was evaluated. Chondrocytes were collected immediately after pressurization (T0), and following 12, 24, and 48 h. Total RNA was extracted, reverse transcribed and used for real-time PCR. At basal condition, a significant increase of miR-155 and miR-181a was observed in OA in comparison to normal cells; on the contrary, no differences in miR-223 and Chuk expression levels were detected between normal and OA chondrocytes. miR-155 and miR-181a resulted significantly downregulated immediately after pressurization (T0) in OA cells. The pressure effect on miR-155 and miR-181a levels was maintained over time. No modifications of miR-223 were observed in response to HP, while Chuk levels resulted significantly reduced at T0 and after 12 h. Pressurization did not cause any modifications in normal cells. In conclusion, HP was able to modulate the expression of miRNA associated to OA pathogenesis. The preliminary results about Chuk response to pressure raised interest in its involvement in the possible HP induced NF-kB pathway modulation. Copyright © 2017 Elsevier Ltd. All rights reserved.

Serum miR-146a and miR-223 as potential new biomarkers for sepsis.

PubMed

Wang, Jia-feng; Yu, Man-li; Yu, Guang; Bian, Jin-jun; Deng, Xiao-ming; Wan, Xiao-jian; Zhu, Ke-ming

2010-03-26

Current biomarkers cannot completely distinguish sepsis from systemic inflammatory response syndrome (SIRS) caused by other non-infectious diseases. Circulating microRNAs (miRNAs) are promising biomarkers for several diseases, but their correlation with sepsis is not totally clarified. Seven miRNAs related to inflammation or infection were included in the present study. Serum miRNA expression was investigated in 50 patients diagnosed with sepsis, 30 patients with SIRS and 20 healthy controls to evaluate the diagnostic and prognostic value. Expression levels of serum miRNAs were determined by quantitative PCR using the Qiagen miScript system. Serum CRP and IL-6 levels were determined by enzyme linked immunosorbent assay. Serum miR-146a and miR-223 were significantly reduced in septic patients compared with SIRS patients and healthy controls. The areas under the receiver operating characteristic curve of miR-146a, miR-223 and IL-6 were 0.858, 0.804 and 0.785, respectively. Serum miR-146a and miR-223 might serve as new biomarkers for sepsis with high specificity and sensitivity. (ClinicalTrials.gov number, NCT00862290.). Copyright (c) 2010 Elsevier Inc. All rights reserved.

Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs.

PubMed

Schmidt, Marco F; Korb, Oliver; Abell, Chris

2017-01-01

microRNAs (miRNAs) have been identified as high-value drug targets. A widely applied strategy in miRNA inhibition is the use of antisense agents. However, it has been shown that oligonucleotides are poorly cell permeable because of their complex chemical structure and due to their negatively charged backbone. Consequently, the general application of oligonucleotides in therapy is limited. Since miRNAs' functions are executed exclusively by the Argonaute 2 protein, we therefore describe a protocol for the design of a novel miRNA inhibitor class: antagonists of the miRNA-Argonaute 2 protein complex, so-called anti-miR-AGOs, that not only block the crucial binding site of the target miRNA but also bind to the protein's active site. Due to their lower molecular weight and, thus, more drug-like chemical structure, the novel inhibitor class may show better pharmacokinetic properties than reported oligonucleotide inhibitors, enabling them for potential therapeutic use.

Independent and combined effects of environmental factors and miR-126, miR-143, and miR-145 on the risk of coronary heart disease.

PubMed

Lin, Da-Cen; Lin, Jia-Bing; Chen, Zhou; Chen, Rong; Wan, Chun-Yu; Lin, Shao-Wei; Ruan, Qi-Shuang; Li, Huang-Yuan; Wu, Si-Ying

2017-11-01

To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD). A frequency-matched case-control study (450 patients, 450 controls) was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, miR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction (PCR) in peripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation. Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD. Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level.

Predicted liquefaction in the greater Oakland area and northern Santa Clara Valley during a repeat of the 1868 Hayward Fault (M6.7-7.0) earthquake

USGS Publications Warehouse

Holzer, Thomas L.; Noce, Thomas E.; Bennett, Michael J.

2010-01-01

Probabilities of surface manifestations of liquefaction due to a repeat of the 1868 (M6.7-7.0) earthquake on the southern segment of the Hayward Fault were calculated for two areas along the margin of San Francisco Bay, California: greater Oakland and the northern Santa Clara Valley. Liquefaction is predicted to be more common in the greater Oakland area than in the northern Santa Clara Valley owing to the presence of 57 km2 of susceptible sandy artificial fill. Most of the fills were placed into San Francisco Bay during the first half of the 20th century to build military bases, port facilities, and shoreline communities like Alameda and Bay Farm Island. Probabilities of liquefaction in the area underlain by this sandy artificial fill range from 0.2 to ~0.5 for a M7.0 earthquake, and decrease to 0.1 to ~0.4 for a M6.7 earthquake. In the greater Oakland area, liquefaction probabilities generally are less than 0.05 for Holocene alluvial fan deposits, which underlie most of the remaining flat-lying urban area. In the northern Santa Clara Valley for a M7.0 earthquake on the Hayward Fault and an assumed water-table depth of 1.5 m (the historically shallowest water level), liquefaction probabilities range from 0.1 to 0.2 along Coyote and Guadalupe Creeks, but are less than 0.05 elsewhere. For a M6.7 earthquake, probabilities are greater than 0.1 along Coyote Creek but decrease along Guadalupe Creek to less than 0.1. Areas with high probabilities in the Santa Clara Valley are underlain by young Holocene levee deposits along major drainages where liquefaction and lateral spreading occurred during large earthquakes in 1868 and 1906.

Estimated suspended-sediment loads and yields in the French and Brandywine Creek Basins, Chester County, Pennsylvania, water years 2008-09

USGS Publications Warehouse

Sloto, Ronald A.; Olson, Leif E.

2011-01-01

Turbidity and suspended-sediment concentration data were collected by the U.S. Geological Survey (USGS) at four stream stations--French Creek near Phoenixville, West Branch Brandywine Creek near Honey Brook, West Branch Brandywine Creek at Modena, and East Branch Brandywine Creek below Downingtown--in Chester County, Pa. Sedimentation and siltation is the leading cause of stream impairment in Chester County, and these data are critical for quantifying sediment transport. This study was conducted by the USGS in cooperation with the Chester County Water Resources Authority and the Chester County Health Department. Data from optical turbidity sensors deployed at the four stations were recorded at 15- or 30-minute intervals by a data logger and uploaded every 1 to 4 hours to the USGS database. Most of the suspended-sediment samples were collected using automated samplers. The use of optical sensors to continuously monitor turbidity provided an accurate estimate of sediment fluctuations without the collection and analysis costs associated with intensive sampling during storms. Turbidity was used as a surrogate for suspended-sediment concentration (SSC), which is a measure of sedimentation and siltation. Regression models were developed between SSC and turbidity for each of the monitoring stations using SSC data collected from the automated samplers and turbidity data collected at each station. Instantaneous suspended-sediment loads (SSL) were computed from time-series turbidity and discharge data for the 2008 and 2009 water years using the regression equations. The instantaneous computations of SSL were summed to provide daily, storm, and water year annual loads. The annual SSL contributed from each basin was divided by the upstream drainage area to estimate the annual sediment yield. For all four basins, storms provided more than 96 percent of the annual SSL. In each basin, four storms generally provided over half the annual SSL each water year. Stormflows with the

Serum-based six-miRNA signature as a potential marker for EC diagnosis: Comparison with TCGA miRNAseq dataset and identification of miRNA-mRNA target pairs by integrated analysis of TCGA miRNAseq and RNAseq datasets.

PubMed

Sharma, Priyanka; Saraya, Anoop; Sharma, Rinu

2018-01-30

To evaluate the diagnostic potential of a six microRNAs (miRNAs) panel consisting of miR-21, miR-144, miR-107, miR-342, miR-93 and miR-152 for esophageal cancer (EC) detection. The expression of miRNAs was analyzed in EC sera samples using quantitative real-time PCR. Risk score analysis was performed and linear regression models were then fitted to generate the six-miRNA panel. In addition, we made an effort to identify significantly dysregulated miRNAs and mRNAs in EC using the Cancer Genome Atlas (TCGA) miRNAseq and RNAseq datasets, respectively. Further, we identified significantly correlated miRNA-mRNA target pairs by integrating TCGA EC miRNAseq dataset with RNAseq dataset. The panel of circulating miRNAs showed enhanced sensitivity (87.5%) and specificity (90.48%) in terms of discriminating EC patients from normal subjects (area under the curve [AUC] = 0.968). Pathway enrichment analysis for potential targets of six miRNAs revealed 48 significant (P < 0.05) pathways, viz. pathways in cancer, mRNA surveillance, MAPK, Wnt, mTOR signaling, and so on. The expression data for mRNAs and miRNAs, downloaded from TCGA database, lead to identification of 2309 differentially expressed genes and 189 miRNAs. Gene ontology and pathway enrichment analysis showed that cell-cycle processes were most significantly enriched for differentially expressed mRNA. Integrated analysis of TCGA miRNAseq and RNAseq datasets resulted in identification of 53 063 significantly and negatively correlated miRNA-mRNA pairs. In summary, a novel and highly sensitive signature of serum miRNAs was identified for EC detection. Moreover, this is the first report identifying miRNA-mRNA target pairs from EC TCGA dataset, thus providing a comprehensive resource for understanding the interactions existing between miRNA and their target mRNAs in EC. © 2018 John Wiley & Sons Australia, Ltd.

75 FR 3962 - Notice of Availability of a Record of Decision (ROD) for the Proposed Bay Area Rapid Transit...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-25

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Notice of Availability of a Record of... Airport (OAK), Oakland, Alameda County, CA AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... public that it has issued a ROD for the proposed construction and operation of the proposed BART...

Contextual view of Fyffe Avenue and Boone Drive. Dispensary (Naval ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Contextual view of Fyffe Avenue and Boone Drive. Dispensary (Naval Medical Center Oakland and Dental Clinic San Francisco Branch Clinics, Building no. 417) is shown at left. Camera facing northwest. - Naval Supply Annex Stockton, Rough & Ready Island, Stockton, San Joaquin County, CA

Contextual view of Fyffe Avenue and Boone Drive. Dispensary (Naval ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Contextual view of Fyffe Avenue and Boone Drive. Dispensary (Naval Medical Center Oakland and Dental Clinic San Francisco Branch Clinics, building no. 417) is shown at the center. Camera facing northeast. - Naval Supply Annex Stockton, Rough & Ready Island, Stockton, San Joaquin County, CA

9. Photocopy of original construction drawing, dated February 1932 (original ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of original construction drawing, dated February 1932 (original print in possession of Veterans Administration, Oakland Branch, Pittsburgh, Pennsylvania). DRAWING 103-26 -- PLOT PLAN -- SHOWING NEW CONSTRUCTION - VA Medical Center, Aspinwall Division, 5103 Delafield Avenue (O'Hara Township), Aspinwall, Allegheny County, PA

300. Frank Deras Jr., Photographer June 1998 VIEW OF THROUGH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

300. Frank Deras Jr., Photographer June 1998 VIEW OF THROUGH TRUSS AND DECK TRUSS SPANS AT PIERS E-8 THROUGH E-l 1, SOUTH SIDE, FACING NORTH-NORTHEAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Evaluation of user perceptions and behaviors of Fast-Trac : pilot study results

DOT National Transportation Integrated Search

1996-01-01

The purpose of the User Perceptions and Behaviors evaluation component of FAST-TRAC is to understand how users perceive and value the in-vehicle navigation system, ALI-SCOUT, and to determine how the system is used in the Oakland County study area. S...

156. Frank Deras Jr., Photographer December 1997 DETAIL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

156. Frank Deras Jr., Photographer December 1997 DETAIL VIEW OF STRAND SHOE AND EYE BAR AT SAN FRANCISCO ANCHORAGE (HUMAN SCALE: TERRY COSTA, CALTRANS EMPLOYEE), FACING SOUTHEAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

MitomiRs in human inflamm-aging: a hypothesis involving miR-181a, miR-34a and miR-146a.

PubMed

Rippo, Maria Rita; Olivieri, Fabiola; Monsurrò, Vladia; Prattichizzo, Francesco; Albertini, Maria Cristina; Procopio, Antonio Domenico

2014-08-01

Mitochondria are intimately involved in the aging process. The decline of autophagic clearance during aging affects the equilibrium between mitochondrial fusion and fission, leading to a build-up of dysfunctional mitochondria, oxidative stress, chronic low-grade inflammation, and increased apoptosis rates, the main hallmarks of aging. Current research suggests that a large number of microRNAs (miRs or miRNAs) are differentially expressed during cell aging. Other lines of evidence indicate that several miRs likely share in "inflamm-aging", an aging-related state characterized by systemic chronic inflammation that in turn provides a biological background favoring susceptibility to age-related diseases and disabilities. Interestingly, miRs can modulate mitochondrial activity, and a discrete miR set has recently been identified in mitochondria of different species and cell types (mitomiRs). Here we show that some mitomiRs (let7b, mir-146a, -133b, -106a, -19b, -20a, -34a, -181a and -221) are also among the miRs primarily involved in cell aging and in inflamm-aging. Of note, Ingenuity Pathway Analysis (IPA) of aging-related mitomiR targets has disclosed a number of resident mitochondrial proteins playing large roles in energy metabolism, mitochondrial transport and apoptosis. Among these, Bcl-2 family members--which are critically involved in maintaining mitochondrial integrity--may play a role in controlling mitochondrial function and dysfunction during cellular aging, also considering that Bcl-2, the master member of the family, is an anti-oxidant and anti-apoptotic factor and regulates mitochondrial fission/fusion and autophagy. This intriguing hypothesis is supported by several observations: i) in endothelial cells undergoing replicative senescence (HUVECs), a well-established model of cell senescence, miR-146a, miR-34a, and miR-181a are over-expressed whereas their target Bcl-2 is down-regulated; ii) IPA of the miR-146a, miR-34a and miR-181a network shows that they

Combination of miRNA499 and miRNA133 Exerts a Synergic Effect on Cardiac Differentiation

PubMed Central

Pisano, Federica; Altomare, Claudia; Cervio, Elisabetta; Barile, Lucio; Rocchetti, Marcella; Ciuffreda, Maria Chiara; Malpasso, Giuseppe; Copes, Francesco; Mura, Manuela; Danieli, Patrizia; Viarengo, Gianluca; Zaza, Antonio; Gnecchi, Massimiliano

2015-01-01

Several studies have demonstrated that miRNA are involved in cardiac development, stem cell maintenance, and differentiation. In particular, it has been shown that miRNA133, miRNA1, and miRNA499 are involved in progenitor cell differentiation into cardiomyocytes. However, it is unknown whether different miRNA may act synergistically to improve cardiac differentiation. We used mouse P19 cells as a cardiogenic differentiation model. miRNA499, miRNA1, or miRNA133 were transiently over-expressed in P19 cells individually or in different combinations. The over-expression of miRNA499 alone increased the number of beating cells and the association of miRNA499 with miRNA133 exerted a synergistic effect, further increasing the number of beating cells. Real-time polymerase chain reaction showed that the combination of miRNA499 + 133 enhanced the expression of cardiac genes compared with controls. Western blot and immunocytochemistry for connexin43 and cardiac troponin T confirmed these findings. Importantly, caffeine responsiveness, a clear functional parameter of cardiac differentiation, was increased by miRNA499 in association with miRNA133 and was directly correlated with the activation of the cardiac troponin I isoform promoter. Cyclic contractions were reversibly abolished by extracellular calcium depletion, nifedipine, ryanodine, and IP3R blockade. Finally, we demonstrated that the use of miRNA499 + 133 induced cardiac differentiation even in the absence of dimethyl sulfoxide. Our results show that the areas spontaneously contracting possess electrophysiological and pharmacological characteristics compatible with true cardiac excitation-contraction coupling. The translational relevance of our findings was reinforced by the demonstration that the over-expression of miRNA499 and miRNA133 was also able to induce the differentiation of human mesenchymal stromal cells toward the cardiac lineage. Stem Cells 2015;33:1187–1199 PMID:25534971

Diagnostic accuracy of serum miR-122 and miR-199a in women with endometriosis.

PubMed

Maged, Ahmed M; Deeb, Wesam S; El Amir, Azza; Zaki, Sherif S; El Sawah, Heba; Al Mohamady, Maged; Metwally, Ahmed A; Katta, Maha A

2018-04-01

To evaluate the value of serum microRNA-122 (miR-122) and miR-199a as reliable noninvasive biomarkers in the diagnosis of endometriosis. During 2015-2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin-6 (IL-6) was detected by enzyme-linked immunosorbent assay and miR-122 and miR-199a expression was measured by quantitative real-time polymerase chain reaction. The serum and PF levels of IL-6, miR-122, and miR-199a were significantly higher in women with endometriosis than in controls (P<0.001 for all comparisons). Serum miR-122 expression was positively correlated with serum IL-6 (r=0.597), PF IL-6 (r=0.603), PF miR-122 (r=0.934), serum miR-199a (r=0.727), and PF miR-199a (r=0.653). Serum miR-199a expression was positively correlated with serum IL-6 (r=0.677), PF IL-6 (r=0.678), PF miR-122 (r=0.744), and PF miR-199a (r=0.932). Serum miR-122 and miR-199a had a sensitivity of 95.6% and 100.0%, and a specificity of 91.4% and 100%, respectively, for the detection of endometriosis. Serum miR-122 and miR-199a were significantly increased in endometriosis, indicating that these microRNAs might serve as biomarkers for the diagnosis of endometriosis. © 2017 International Federation of Gynecology and Obstetrics.

DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

EPA Science Inventory

This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs

PubMed Central

Nepal, Chirag; Coolen, Marion; Hadzhiev, Yavor; Cussigh, Delphine; Mydel, Piotr; Steen, Vidar M.; Carninci, Piero; Andersen, Jesper B.; Bally-Cuif, Laure; Müller, Ferenc; Lenhard, Boris

2016-01-01

MicroRNAs (miRNAs) play a major role in the post-transcriptional regulation of target genes, especially in development and differentiation. Our understanding about the transcriptional regulation of miRNA genes is limited by inadequate annotation of primary miRNA (pri-miRNA) transcripts. Here, we used CAGE-seq and RNA-seq to provide genome-wide identification of the pri-miRNA core promoter repertoire and its dynamic usage during zebrafish embryogenesis. We assigned pri-miRNA promoters to 152 precursor-miRNAs (pre-miRNAs), the majority of which were supported by promoter associated post-translational histone modifications (H3K4me3, H2A.Z) and RNA polymerase II (RNAPII) occupancy. We validated seven miR-9 pri-miRNAs by in situ hybridization and showed similar expression patterns as mature miR-9. In addition, processing of an alternative intronic promoter of miR-9–5 was validated by 5′ RACE PCR. Developmental profiling revealed a subset of pri-miRNAs that are maternally inherited. Moreover, we show that promoter-associated H3K4me3, H2A.Z and RNAPII marks are not only present at pri-miRNA promoters but are also specifically enriched at pre-miRNAs, suggesting chromatin level regulation of pre-miRNAs. Furthermore, we demonstrated that CAGE-seq also detects 3′-end processing of pre-miRNAs on Drosha cleavage site that correlates with miRNA-offset RNAs (moRNAs) production and provides a new tool for detecting Drosha processing events and predicting pre-miRNA processing by a genome-wide assay. PMID:26673698

miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

DOE Office of Scientific and Technical Information (OSTI.GOV)

McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk; Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL; Patel, Daksha, E-mail: d.patel@qub.ac.uk

2014-01-05

A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in themore » cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.« less

miRNA Expression Profile after Status Epilepticus and Hippocampal Neuroprotection by Targeting miR-132

PubMed Central

Jimenez-Mateos, Eva M.; Bray, Isabella; Sanz-Rodriguez, Amaya; Engel, Tobias; McKiernan, Ross C.; Mouri, Genshin; Tanaka, Katsuhiro; Sano, Takanori; Saugstad, Julie A.; Simon, Roger P.; Stallings, Raymond L.; Henshall, David C.

2011-01-01

When an otherwise harmful insult to the brain is preceded by a brief, noninjurious stimulus, the brain becomes tolerant, and the resulting damage is reduced. Epileptic tolerance develops when brief seizures precede an episode of prolonged seizures (status epilepticus). MicroRNAs (miRNAs) are small, noncoding RNAs that function as post-transcriptional regulators of gene expression. We investigated how prior seizure preconditioning affects the miRNA response to status epilepticus evoked by intra-amygdalar kainic acid in mice. The miRNA was extracted from the ipsilateral CA3 subfield 24 hours after focal-onset status epilepticus in animals that had previously received either seizure preconditioning (tolerance) or no preconditioning (injury), and mature miRNA levels were measured using TaqMan low-density arrays. Expression of 21 miRNAs was increased, relative to control, after status epilepticus alone, and expression of 12 miRNAs was decreased. Increased miR-132 levels were matched with increased binding to Argonaute-2, a constituent of the RNA-induced silencing complex. In tolerant animals, expression responses of >40% of the injury-group-detected miRNAs differed, being either unchanged relative to control or down-regulated, and this included miR-132. In vivo microinjection of locked nucleic acid-modified oligonucleotides (antagomirs) against miR-132 depleted hippocampal miR-132 levels and reduced seizure-induced neuronal death. Thus, our data strongly suggest that miRNAs are important regulators of seizure-induced neuronal death. PMID:21945804

American Recovery and Reinvestment Act (ARRA) FEMP Technical Assistance Federal Aviation Administration – Project 209 Control Tower and Support Building Oakland, CA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arends, J.; Sandusky, William F.

2010-03-01

This report represents findings of a design review team that evaluated construction documents (at the 70% level) and operating specifications for a new control tower and support building that will be build at Oakland, California by the Federal Aviation Administration (FAA). The focus of the review was to identify measures that could be incorporated into the final design and operating specification that would result in additional energy savings for the FAA that would not have otherwise occurred.

Differentiation of umbilical cord derived mesenchymal stem cells to hepatocyte cells by transfection of miR-106a, miR-574-3p, and miR-451.

PubMed

Khosravi, Maryam; Azarpira, Negar; Shamdani, Sara; Hojjat-Assari, Suzzan; Naserian, Sina; Karimi, Mohammad Hossein

2018-08-15

Studying the profile of micro RNAs (miRs) elucidated the highest expressed miRs in hepatic differentiation. In this study, we investigated to clarify the role of three embryonic overexpressed miRs (miR-106a, miR-574-3p and miR-451) during hepatic differentiation of human umbilical cord derived mesenchymal stem cells (UC-MSCs). We furthermore, aimed to explore whether overexpression of any of these miRs alone is sufficient to induce the differentiation of the UC-MSCs into hepatocyte-like cells. UC-MSCs were transfected either alone or together with miR-106a, miR-574-3p and miR-451 and their potential hepatic differentiation and alteration in gene expression profile, morphological changes and albumin secretion ability were investigated. We found that up-regulation of any of these three miRs alone cannot induce expression of all hepatic specific genes. Transfection of each miR alone, led to Sox17, FoxA2 expression that are related to initiation step of hepatic differentiation. However, concurrent ectopic overexpression of three miRs together can induce UC-MSCs differentiation into functionally mature hepatocytes. These results show that miRs have the capability to directly convert UC-MSCs to a hepatocyte phenotype in vitro. Copyright © 2018. Published by Elsevier B.V.

miRNet - dissecting miRNA-target interactions and functional associations through network-based visual analysis

PubMed Central

Fan, Yannan; Siklenka, Keith; Arora, Simran K.; Ribeiro, Paula; Kimmins, Sarah; Xia, Jianguo

2016-01-01

MicroRNAs (miRNAs) can regulate nearly all biological processes and their dysregulation is implicated in various complex diseases and pathological conditions. Recent years have seen a growing number of functional studies of miRNAs using high-throughput experimental technologies, which have produced a large amount of high-quality data regarding miRNA target genes and their interactions with small molecules, long non-coding RNAs, epigenetic modifiers, disease associations, etc. These rich sets of information have enabled the creation of comprehensive networks linking miRNAs with various biologically important entities to shed light on their collective functions and regulatory mechanisms. Here, we introduce miRNet, an easy-to-use web-based tool that offers statistical, visual and network-based approaches to help researchers understand miRNAs functions and regulatory mechanisms. The key features of miRNet include: (i) a comprehensive knowledge base integrating high-quality miRNA-target interaction data from 11 databases; (ii) support for differential expression analysis of data from microarray, RNA-seq and quantitative PCR; (iii) implementation of a flexible interface for data filtering, refinement and customization during network creation; (iv) a powerful fully featured network visualization system coupled with enrichment analysis. miRNet offers a comprehensive tool suite to enable statistical analysis and functional interpretation of various data generated from current miRNA studies. miRNet is freely available at http://www.mirnet.ca. PMID:27105848

miRSponge: a manually curated database for experimentally supported miRNA sponges and ceRNAs.

PubMed

Wang, Peng; Zhi, Hui; Zhang, Yunpeng; Liu, Yue; Zhang, Jizhou; Gao, Yue; Guo, Maoni; Ning, Shangwei; Li, Xia

2015-01-01

In this study, we describe miRSponge, a manually curated database, which aims at providing an experimentally supported resource for microRNA (miRNA) sponges. Recent evidence suggests that miRNAs are themselves regulated by competing endogenous RNAs (ceRNAs) or 'miRNA sponges' that contain miRNA binding sites. These competitive molecules can sequester miRNAs to prevent them interacting with their natural targets to play critical roles in various biological and pathological processes. It has become increasingly important to develop a high quality database to record and store ceRNA data to support future studies. To this end, we have established the experimentally supported miRSponge database that contains data on 599 miRNA-sponge interactions and 463 ceRNA relationships from 11 species following manual curating from nearly 1200 published articles. Database classes include endogenously generated molecules including coding genes, pseudogenes, long non-coding RNAs and circular RNAs, along with exogenously introduced molecules including viral RNAs and artificial engineered sponges. Approximately 70% of the interactions were identified experimentally in disease states. miRSponge provides a user-friendly interface for convenient browsing, retrieval and downloading of dataset. A submission page is also included to allow researchers to submit newly validated miRNA sponge data. Database URL: http://www.bio-bigdata.net/miRSponge. © The Author(s) 2015. Published by Oxford University Press.

miRSponge: a manually curated database for experimentally supported miRNA sponges and ceRNAs

PubMed Central

Wang, Peng; Zhi, Hui; Zhang, Yunpeng; Liu, Yue; Zhang, Jizhou; Gao, Yue; Guo, Maoni; Ning, Shangwei; Li, Xia

2015-01-01

In this study, we describe miRSponge, a manually curated database, which aims at providing an experimentally supported resource for microRNA (miRNA) sponges. Recent evidence suggests that miRNAs are themselves regulated by competing endogenous RNAs (ceRNAs) or ‘miRNA sponges’ that contain miRNA binding sites. These competitive molecules can sequester miRNAs to prevent them interacting with their natural targets to play critical roles in various biological and pathological processes. It has become increasingly important to develop a high quality database to record and store ceRNA data to support future studies. To this end, we have established the experimentally supported miRSponge database that contains data on 599 miRNA-sponge interactions and 463 ceRNA relationships from 11 species following manual curating from nearly 1200 published articles. Database classes include endogenously generated molecules including coding genes, pseudogenes, long non-coding RNAs and circular RNAs, along with exogenously introduced molecules including viral RNAs and artificial engineered sponges. Approximately 70% of the interactions were identified experimentally in disease states. miRSponge provides a user-friendly interface for convenient browsing, retrieval and downloading of dataset. A submission page is also included to allow researchers to submit newly validated miRNA sponge data. Database URL: http://www.bio-bigdata.net/miRSponge. PMID:26424084

Expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells, follicular fluid, and serum of women with polycystic ovary syndrome (PCOS).

PubMed

Naji, Mohammad; Nekoonam, Saeid; Aleyasin, Ashraf; Arefian, Ehsan; Mahdian, Reza; Azizi, Elham; Shabani Nashtaei, Maryam; Amidi, Fardin

2018-01-01

Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that affects women in reproductive age. MicroRNAs (miRNAs) play crucial roles in normal function of female reproductive system and folliculogenesis. Deregulated expression of miRNAs in PCOS condition may be significantly implicated in the pathogenesis of PCOS. We determined relative expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells (GLCs), follicular fluid (FF), and serum of PCOS patients. Human subjects were divided into PCOS (n = 20) and control (n = 21) groups. GLCs, FF, and serum were isolated and stored. RNA isolation was performed and cDNA was reversely transcribed using specific stem-loop RT primers. Relative expression of miRNAs was calculated after normalization against U6 expression. Correlation of miRNAs' expression level with basic clinical features and predictive value of miRNAs in FF and serum were appraised. Relative expression of miR-145 and miR-182 in GLCs was significantly decreased in PCOS, but miR-182 in FF of PCOS patients revealed up-regulated levels. Significant correlations between level of miRNAs in FF and serum and hormonal profile of subjects were observed. MiR-182 in FF showed a significant predictive value with AUC of 0.73, 76.4% sensitivity, and 70.5% specificity which was improved after combination of miR-182 and miR-145. A significant dysregulation of miR-145 and miR-182 in GLCs of PCOS may indicate their involvement in pathogenesis of PCOS. Differential up-regulation of miR-182 in FF of PCOS patients with its promising predictive values for discrimination of PCOS reinforced the importance of studying miRNAs' profile in FF.

miRNA Expression profile after status epilepticus and hippocampal neuroprotection by targeting miR-132.

PubMed

Jimenez-Mateos, Eva M; Bray, Isabella; Sanz-Rodriguez, Amaya; Engel, Tobias; McKiernan, Ross C; Mouri, Genshin; Tanaka, Katsuhiro; Sano, Takanori; Saugstad, Julie A; Simon, Roger P; Stallings, Raymond L; Henshall, David C

2011-11-01

When an otherwise harmful insult to the brain is preceded by a brief, noninjurious stimulus, the brain becomes tolerant, and the resulting damage is reduced. Epileptic tolerance develops when brief seizures precede an episode of prolonged seizures (status epilepticus). MicroRNAs (miRNAs) are small, noncoding RNAs that function as post-transcriptional regulators of gene expression. We investigated how prior seizure preconditioning affects the miRNA response to status epilepticus evoked by intra-amygdalar kainic acid in mice. The miRNA was extracted from the ipsilateral CA3 subfield 24 hours after focal-onset status epilepticus in animals that had previously received either seizure preconditioning (tolerance) or no preconditioning (injury), and mature miRNA levels were measured using TaqMan low-density arrays. Expression of 21 miRNAs was increased, relative to control, after status epilepticus alone, and expression of 12 miRNAs was decreased. Increased miR-132 levels were matched with increased binding to Argonaute-2, a constituent of the RNA-induced silencing complex. In tolerant animals, expression responses of >40% of the injury-group-detected miRNAs differed, being either unchanged relative to control or down-regulated, and this included miR-132. In vivo microinjection of locked nucleic acid-modified oligonucleotides (antagomirs) against miR-132 depleted hippocampal miR-132 levels and reduced seizure-induced neuronal death. Thus, our data strongly suggest that miRNAs are important regulators of seizure-induced neuronal death. Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Bay Area Tumor Institute NCORP | Division of Cancer Prevention

Cancer.gov

The Bay Area Tumor Institute NCI Community Oncology Research Program (BATI NCORP) is a consortium of three community and county hospitals and four private physician practices in Greater Oakland, California. It was founded in 1979 as a CGOP and converted to CCOP status in 1989. |

Narcolepsy patients' blood-based miRNA expression profiling: miRNA expression differences with Pandemrix vaccination.

PubMed

Mosakhani, N; Sarhadi, V; Panula, P; Partinen, M; Knuutila, S

2017-11-01

Narcolepsy is a neurological sleep disorder characterized by excessive daytime sleepiness and nighttime sleep disturbance. Among children and adolescents vaccinated with Pandemrix vaccine in Finland and Sweden, the number of narcolepsy cases increased. Our aim was to identify miRNAs involved in narcolepsy and their association with Pandemrix vaccination. We performed global miRNA proofing by miRNA microarrays followed by RT-PCR verification on 20 narcolepsy patients (Pandemrix-associated and Pandemrix-non-associated) and 17 controls (vaccinated and non-vaccinated). Between all narcolepsy patients and controls, 11 miRNAs were differentially expressed; 17 miRNAs showed significantly differential expression between Pandemrix-non-associated narcolepsy patients and non-vaccinated healthy controls. MiR-188-5p and miR-4499 were over-expressed in narcolepsy patients vs healthy controls. Two miRNAs, miR-1470 and miR-4455, were under-expressed in Pandemrix-associated narcolepsy patients vs Pandemrix-non-associated narcolepsy patients. We identified miRNA expression patterns in narcolepsy patients that linked them to mRNA targets known to be involved in brain-related pathways or brain disorders. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

miR-205 and miR-200c: Predictive Micro RNAs for Lymph Node Metastasis in Triple Negative Breast Cancer

PubMed Central

Yilmaz, Ismail; Narli, Gizem; Haholu, Aptullah; Kucukodaci, Zafer; Demirel, Dilaver

2014-01-01

Purpose We examined expression profiles of 16 micro RNAs (miRNAs) in triple negative breast cancers to identify their potential as biomarkers for lymph node metastasis. Methods The expression profiles of miR-9, miR-21, miR-30a, miR-30d, miR-31, miR-34a, miR-34c, miR-100, miR-122, miR-125b, miR-146a, miR-146b, miR-155, miR-181a, miR-200c, and miR-205 were examined by using real-time quantitative reverse transcription polymerase chain reaction in tumor samples and corresponding benign breast tissues. Their associations with histopathological features and prognostic parameters were assessed. Results When compared with the expression in benign breast tissues, seven of the miRNAs (miR-31, miR-205, miR-34a, miR-146a, miR-125b, miR-34c, and miR-181a) were downregulated more than 1.5-fold in tumor tissues, whereas, only miR-21 was found to be upregulated more than 1.5-fold in tumor tissues. Although miR-200c levels were decreased only 1.12-fold in tumor tissues, the reduced expressions of miR-200c and miR-205 were significantly associated with lymph node metastasis (p=0.021 and p=0.016, respectively). Conclusion Our results demonstrate that miR-205 and miR-200c expression levels may be useful in predicting lymph node metastasis in triple negative breast cancer patients. PMID:25013435

Expression Levels and Clinical Significance of miR-21-5p, miR-let-7a, and miR-34c-5p in Laryngeal Squamous Cell Carcinoma

PubMed Central

Gioacchini, Federico M.; Çeka, Artan; Rubini, Corrado; Ferrante, Luigi; Procopio, Antonio D.; Olivieri, Fabiola

2017-01-01

Objective Altered microRNAs (miRNAs) expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). The aim of this study was to determine the role and clinical value of three LSCC-related miRs, such as miR-21-5p, miR-let-7a, and miR-34c-5p in a homogeneous cohort of patients with primary LSCC treated by primary surgery. Methods Expression levels of miR-21-5p, miR-let-7a, and miR-34c-5p were detected in 43 pairs of LSCC and adjacent normal tissues by reverse-transcription quantitative PCR. Overall survival and disease-free survival were evaluated using the Kaplan–Meier method, and multivariate analysis was performed using the Cox proportional hazard analysis. Results miR-21-5p is significantly upregulated, while miR-let-7a is significantly downregulated in LSCC tumor tissues compared with the corresponding adjacent normal tissues. The downregulation of miR-34c-5p expression significantly correlated with a shorter disease-free survival and, in the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence. Conclusions miR-21-5p, miR-let-7a, and miR-34c-5p seem to play a critical role in LSCC carcinogenesis and might have a diagnostic and prognostic clinical value. The miR-let-7a levels could have a predictive role for lymph node metastases and miR-34c-5p might be a promising biomarker of patient outcome. PMID:29082244

miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai

2011-03-25

Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target themore » retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.« less

47 CFR 90.311 - Frequencies.

Code of Federal Regulations, 2011 CFR

2011-10-01

... MOBILE RADIO SERVICES Authorization in the Band 470-512 MHz (UHF-TV Sharing) § 90.311 Frequencies. (a.... NJ Pittsburgh, PA 470.30625 to 472.99375 473.30625 to 475.99375 Los Angeles, CA 470.05625 to 472....99375 479.30625 to 481.99375 16 Boston, MADallas/Fort Worth, TX Detroit, MI San Francisco/Oakland, CA...

47 CFR 90.311 - Frequencies.

Code of Federal Regulations, 2013 CFR

2013-10-01

... MOBILE RADIO SERVICES Authorization in the Band 470-512 MHz (UHF-TV Sharing) § 90.311 Frequencies. (a.... NJ Pittsburgh, PA 470.30625 to 472.99375 473.30625 to 475.99375 Los Angeles, CA 470.05625 to 472....99375 479.30625 to 481.99375 16 Boston, MADallas/Fort Worth, TX Detroit, MI San Francisco/Oakland, CA...

MiR-578 and miR-573 as potential players in BRCA-related breast cancer angiogenesis

PubMed Central

Danza, Katia; Summa, Simona De; Pinto, Rosamaria; Pilato, Brunella; Palumbo, Orazio; Merla, Giuseppe; Simone, Gianni; Tommasi, Stefania

2015-01-01

The involvement of microRNA (miRNAs), a new class of small RNA molecules, in governing angiogenesis has been well described. Our aim was to investigate miRNA-mediated regulation of angiogenesis in a series of familial breast cancers stratified by BRCA1/2 mutational status in BRCA carriers and BRCA non-carriers (BRCAX). Affymetrix GeneChip miRNA Arrays were used to perform miRNA expression analysis on 43 formalin-fixed paraffin-embedded (FFPE) tumour tissue familial breast cancers (22 BRCA 1/2-related and 21 BRCAX). Pathway enrichment analysis was carried out with the DIANA miRPath v2.0 web-based computational tool, and the miRWalk database was used to identify target genes of deregulated miRNAs. An independent set of 8 BRCA 1/2-related and 11 BRCAX breast tumors was used for validation by Real-Time PCR. In vitro analysis on HEK293, MCF-7 and SUM149PT cells were performed to best-clarify miR-573 and miR-578 role. A set of 16 miRNAs differentially expressed between BRCA 1/2-related and BRCAX breast tumors emerged from the profile analysis. Among these, miR-578 and miR-573 were found to be down-regulated in BRCA 1/2-related breast cancer and associated to the Focal adhesion, Vascular Endothelial Growth Factor (VEGF) and Hypoxia Inducible Factor-1 (HIF-1) signaling pathways. Our data highlight the role of miR-578 and miR-573 in controlling BRCA 1/2-related angiogenesis by targeting key regulators of Focal adhesion, VEGF and HIF-1 signaling pathways. PMID:25333258

Expression of miR-155, miR-146a, and miR-326 in T1D patients from Chile: relationship with autoimmunity and inflammatory markers.

PubMed

García-Díaz, Diego F; Pizarro, Carolina; Camacho-Guillén, Patricia; Codner, Ethel; Soto, Néstor; Pérez-Bravo, Francisco

2018-02-01

Objective The aim of this research was to analyze the expression profile of miR-155, miR-146a, and miR-326 in peripheral blood mononuclear cells (PBMC) of 47 patients with type 1 diabetes mellitus (T1D) and 39 control subjects, as well as the possible association with autoimmune or inflammatory markers. Subjects and methods Expression profile of miRs by means of qPCR using TaqMan probes. Autoantibodies and inflammatory markers by ELISA. Statistical analysis using bivariate correlation. Results The analysis of the results shows an increase in the expression of miR-155 in T1D patients in basal conditions compared to the controls (p < 0.001) and a decreased expression level of miR-326 (p < 0.01) and miR-146a (p < 0.05) compared T1D patients to the controls. miR-155 was the only miRs associated with autoinmmunity (ZnT8) and inflammatory status (vCAM). Conclusion Our data show a possible role of miR-155 related to autoimmunity and inflammation in Chilean patients with T1D.

Hydrogeology of the surficial and intermediate aquifers of central Sarasota County, Florida

USGS Publications Warehouse

Duerr, A.D.; Wolansky, R.M.

1986-01-01

The geohydrologic units underlying a 300 sq mi area in central Sarasota County, Florida, consist of the surficial aquifer, intermediate aquifers (Tamiami-upper Hawthorn and lower Hawthorn-upper Tampa aquifers) and confining units, the Floridan aquifer system, and the sub-Floridan confining unit. The saturated thickness of the surficial aquifer ranges from about 40 to 75 ft and the water table is generally within 5 ft of land surface. The Tamiami-upper Hawthorn is the uppermost intermediate aquifer. The top of the aquifer ranges from about 50 ft to about 75 below sea level and has an average thickness of about 100 ft. The lower Hawthorne-upper Tampa aquifer is the lowermost intermediate aquifer. The top of the aquifer ranges from about 190 to about 220 ft below sea level and its thickness ranges from about 200 to 250 ft. The quality of water in the surficial and the two intermediate aquifers is acceptable for potable use except near the coast. Water from the Floridan aquifer system is used primarily for agricultural purposes because it is too mineralized for most other uses; therefore, the surficial and intermediate aquifers are developed for water supply. The artesian pressure of the various aquifers generally increases with depth. A more detailed geohydrologic description is presented for the Ringling-MacArthur Reserve, a 51 sq mi area in the central part of the county that may be used by Sarasota County as a future water supply. Average annual rainfall is 56 inches and evapotranspiration is about 42 in at the Reserve. The area has a high water table, many sloughs and swamps, and undeveloped land, making it an attractive site as a potential source of water. (Author 's abstract)

Dysregulation of miR-31 and miR-21 induced by zinc deficiency promotes esophageal cancer

PubMed Central

Croce, Carlo M; Fong, Louise Y.Y

2012-01-01

Zinc deficiency (ZD) increases the risk of esophageal squamous cell carcinoma (ESCC). In a rat model, chronic ZD induces an inflammatory gene signature that fuels ESCC development. microRNAs regulate gene expression and are aberrantly expressed in cancers. Here we investigated whether chronic ZD (23 weeks) also induces a protumorigenic microRNA signature. Using the nanoString technology, we evaluated microRNA profiles in ZD esophagus and six additional tissues (skin, lung, pancreas, liver, prostate and peripheral blood mononuclear cells [PBMC]). ZD caused overexpression of inflammation genes and altered microRNA expression across all tissues analyzed, predictive of disease development. Importantly, the inflammatory ZD esophagus had a distinct microRNA signature resembling human ESCC or tongue SCC miRNAomes with miR-31 and miR-21 as the top-up-regulated species. Circulating miR-31 was also the top-up-regulated species in PBMCs. In ZD esophagus and tongue, oncogenic miR-31 and miR-21 overexpression was accompanied by down-regulation of their respective tumor-suppressor targets PPP2R2A and PDCD4. Importantly, esophageal miR-31 and miR-21 levels were directly associated with the appearance of ESCC in ZD rats, as compared with their cancer-free Zn-sufficient or Zn-replenished counterparts. In situ hybridization analysis in rat and human tongue SCCs localized miR-31 to tumor cells and miR-21 to stromal cells. In regressing tongue SCCs from Zn-supplemented rats, miR-31 and miR-21 expression was concomitantly reduced, establishing their responsiveness to Zn therapy. A search for putative microRNA targets revealed a bias toward genes in inflammatory pathways. Our finding that ZD causes miR-31 and miR-21 dysregulation associated with inflammation provides insight into mechanisms whereby ZD promotes ESCC. PMID:22689922

Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita.

PubMed

Cui, Ruqiang; Zhang, Lei; Chen, Yuyan; Huang, Wenkun; Fan, Chengming; Wu, Qingsong; Peng, Deliang; da Silva, Washington; Sun, Xiaotang

2017-05-01

The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein sequence had 72% amino acid similarity with that of Ditylenchus destructor Dd-AChE-3. Phylogenetic analyses using 41 AChEs from 24 species showed that Mi-ACE-3 formed a cluster with 4 other nematode AChEs. Our results revealed that the Mi-ace-3 cloned in this study, which is orthologous to Caenorhabditis elegans AChE, belongs to the nematode ACE-3/4 subgroup. There was a significant reduction in the number of galls in transgenic tobacco roots when Mi-ace-1, Mi-ace-2, and Mi-ace-3 were knocked down simultaneously, whereas little or no effect were observed when only one or two of these genes were knocked down. This is an indication that the functions of these three genes are redundant. Copyright © 2017. Published by Elsevier Inc.

Ancient human miRNAs are more likely to have broad functions and disease associations than young miRNAs.

PubMed

Patel, Vir D; Capra, John A

2017-08-31

microRNAs (miRNAs) are essential to the regulation of gene expression in eukaryotes, and improper expression of miRNAs contributes to hundreds of diseases. Despite the essential functions of miRNAs, the evolutionary dynamics of how they are integrated into existing gene regulatory and functional networks is not well understood. Knowledge of the origin and evolutionary history a gene has proven informative about its functions and disease associations; we hypothesize that incorporating the evolutionary origins of miRNAs into analyses will help resolve differences in their functional dynamics and how they influence disease. We computed the phylogenetic age of miRNAs across 146 species and quantified the relationship between human miRNA age and several functional attributes. Older miRNAs are significantly more likely to be associated with disease than younger miRNAs, and the number of associated diseases increases with age. As has been observed for genes, the miRNAs associated with different diseases have different age profiles. For example, human miRNAs implicated in cancer are enriched for origins near the dawn of animal multicellularity. Consistent with the increasing contribution of miRNAs to disease with age, older miRNAs target more genes than younger miRNAs, and older miRNAs are expressed in significantly more tissues. Furthermore, miRNAs of all ages exhibit a strong preference to target older genes; 93% of validated miRNA gene targets were in existence at the origin of the targeting miRNA. Finally, we find that human miRNAs in evolutionarily related families are more similar in their targets and expression profiles than unrelated miRNAs. Considering the evolutionary origin and history of a miRNA provides useful context for the analysis of its function. Consistent with recent work in Drosophila, our results support a model in which miRNAs increase their expression and functional regulatory interactions over evolutionary time, and thus older miRNAs have increased

Three novel serum biomarkers, miR-1, miR-133a, and miR-206 for Limb-girdle muscular dystrophy, Facioscapulohumeral muscular dystrophy, and Becker muscular dystrophy.

PubMed

Matsuzaka, Yasunari; Kishi, Soichiro; Aoki, Yoshitsugu; Komaki, Hirofumi; Oya, Yasushi; Takeda, Shin-Ichi; Hashido, Kazuo

2014-11-01

Muscular dystrophies are a clinically and genetically heterogeneous group of inherited myogenic disorders. In clinical tests for these diseases, creatine kinase (CK) is generally used as diagnostic blood-based biomarker. However, because CK levels can be altered by various other factors, such as vigorous exercise, etc., false positive is observed. Therefore, three microRNAs (miRNAs), miR-1, miR-133a, and miR-206, were previously reported as alternative biomarkers for duchenne muscular dystrophy (DMD). However, no alternative biomarkers have been established for the other muscular dystrophies. We, therefore, evaluated whether these miR-1, miR-133a, and miR-206 can be used as powerful biomarkers using the serum from muscular dystrophy patients including DMD, myotonic dystrophy 1 (DM1), limb-girdle muscular dystrophy (LGMD), facioscapulohumeral muscular dystrophy (FSHD), becker muscular dystrophy (BMD), and distal myopathy with rimmed vacuoles (DMRV) by qualitative polymerase chain reaction (PCR) amplification assay. Statistical analysis indicated that all these miRNA levels in serum represented no significant differences between all muscle disorders examined in this study and controls by Bonferroni correction. However, some of these indicated significant differences without correction for testing multiple diseases (P < 0.05). The median values of miR-1 levels in the serum of patients with LGMD, FSHD, and BMD were approximately 5.5, 3.3 and 1.7 compared to that in controls, 0.68, respectively. Similarly, those of miR-133a and miR-206 levels in the serum of BMD patients were about 2.5 and 2.1 compared to those in controls, 1.03 and 1.32, respectively. Taken together, our data demonstrate that levels of miR-1, miR-133a, and miR-206 in serum of BMD and miR-1 in sera of LGMD and FSHD patients showed no significant differences compared with those of controls by Bonferroni correction. However, the results might need increase in sample sizes to evaluate these three miRNAs as

28. VIEW OF THE SOLDERING NICHE FORMED WITH BRICKS. THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

28. VIEW OF THE SOLDERING NICHE FORMED WITH BRICKS. THE BACK OF THE NICHE IS CEMENT FINISHED. THE BOTTOM HAS A 1 INCH THICK ASBESTOS SHELF. THIS PHOTO WAS TAKEN AT THE 3RD FLOOR. - Pacific Telephone & Telegraph Company Building, 1519 Franklin Street, Oakland, Alameda County, CA

13. Photocopy of original construction drawing, dated November 1932 (original ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Photocopy of original construction drawing, dated November 1932 (original print in possession of Veterans Administration, Oakland Branch, Pittsburgh, Pennsylvania). DRAWING 32-3 -- ADMINISTRATION BUILDING, BUILDING NO. 32 -- FIRST AND SECOND FLOOR PLANS. - VA Medical Center, Aspinwall Division, Administration Building, 5103 Delafield Avenue, Aspinwall, Allegheny County, PA

12. Photocopy of original construction drawing, dated November 1932 (original ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Photocopy of original construction drawing, dated November 1932 (original print in possession of Veterans Administration, Oakland Branch, Pittsburgh, Pennsylvania). DRAWING 32-2 -- ADMINISTRATION BUILDING, BUILDING NO. 32 -- BASEMENT PLAN AND CUPOLA DETAIL. - VA Medical Center, Aspinwall Division, Administration Building, 5103 Delafield Avenue, Aspinwall, Allegheny County, PA

20. TYPICAL VIEW OF FRONT WINDOWS FROM 4TH TO 9TH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. TYPICAL VIEW OF FRONT WINDOWS FROM 4TH TO 9TH FLOOR WITH WHITE GLAZED TERRA COTTA SILL AND HEADERS. MULLIONS ARE ORANGE BROWN BRICKS LIKE THE WALLS. BRICKS ARE IN FLEMISH BOND PATTERN. - Pacific Telephone & Telegraph Company Building, 1519 Franklin Street, Oakland, Alameda County, CA

Instructional Program Planning for Outdoor Education.

ERIC Educational Resources Information Center

Garbutt, Barbara; And Others

A guide on instructional program planning for outdoor education, prepared for Oakland County (Michigan) teachers, contains suggestions for program planning and lists information sources on suitable outdoor activities. The purpose of outdoor education is defined as being: "to enrich, vitalize and complement content areas of the school…

Novel Triazole linked 2-phenyl benzoxazole derivatives induce apoptosis by inhibiting miR-2, miR-13 and miR-14 function in Drosophila melanogaster.

PubMed

Mondal, Tanmoy; Lavanya, A V S; Mallick, Akash; Dadmala, Tulshiram L; Kumbhare, Ravindra M; Bhadra, Utpal; Bhadra, Manika Pal

2017-06-01

Apoptosis is an important phenomenon in multi cellular organisms for maintaining tissue homeostasis and embryonic development. Defect in apoptosis leads to a number of disorders like- autoimmune disorder, immunodeficiency and cancer. 21-22 nucleotides containing micro RNAs (miRNAs/miRs) function as a crucial regulator of apoptosis alike other cellular pathways. Recently, small molecules have been identified as a potent inducer of apoptosis. In this study, we have identified novel Triazole linked 2-phenyl benzoxazole derivatives (13j and 13h) as a negative regulator of apoptosis inhibiting micro RNAs (miR-2, miR-13 and miR-14) in a well established in vivo model Drosophila melanogaster where the process of apoptosis is very similar to human apoptosis. These compounds inhibit miR-2, miR-13 and miR-14 activity at their target sites, which induce an increased caspase activity, and in turn influence the caspase dependent apoptotic pathway. These two compounds also increase the mitochondrial reactive oxygen species (ROS) level to trigger apoptotic cell death.

Bioinformatics of cardiovascular miRNA biology.

PubMed

Kunz, Meik; Xiao, Ke; Liang, Chunguang; Viereck, Janika; Pachel, Christina; Frantz, Stefan; Thum, Thomas; Dandekar, Thomas

2015-12-01

MicroRNAs (miRNAs) are small ~22 nucleotide non-coding RNAs and are highly conserved among species. Moreover, miRNAs regulate gene expression of a large number of genes associated with important biological functions and signaling pathways. Recently, several miRNAs have been found to be associated with cardiovascular diseases. Thus, investigating the complex regulatory effect of miRNAs may lead to a better understanding of their functional role in the heart. To achieve this, bioinformatics approaches have to be coupled with validation and screening experiments to understand the complex interactions of miRNAs with the genome. This will boost the subsequent development of diagnostic markers and our understanding of the physiological and therapeutic role of miRNAs in cardiac remodeling. In this review, we focus on and explain different bioinformatics strategies and algorithms for the identification and analysis of miRNAs and their regulatory elements to better understand cardiac miRNA biology. Starting with the biogenesis of miRNAs, we present approaches such as LocARNA and miRBase for combining sequence and structure analysis including phylogenetic comparisons as well as detailed analysis of RNA folding patterns, functional target prediction, signaling pathway as well as functional analysis. We also show how far bioinformatics helps to tackle the unprecedented level of complexity and systemic effects by miRNA, underlining the strong therapeutic potential of miRNA and miRNA target structures in cardiovascular disease. In addition, we discuss drawbacks and limitations of bioinformatics algorithms and the necessity of experimental approaches for miRNA target identification. This article is part of a Special Issue entitled 'Non-coding RNAs'. Copyright © 2014 Elsevier Ltd. All rights reserved.

[The role of miRNA in endometrial cancer in the context of miRNA 205].

PubMed

Wilczyński, Miłosz; Danielska, Justyna; Dzieniecka, Monika; Malinowski, Andrzej

2015-11-01

MiRNAs are small, non-coding molecules of ribonucleic acids of approximately 22 bp length, which serve as regulators of gene expression and protein translation due to interference with messenger RNA (mRNA). MiRNAs, which take part in the regulation of cell cycle and apoptosis, may be associated with carcinogenesis. Aberrant expression of miRNAs in endometrial cancer might contribute to the endometrial cancer initiation or progression, as well as metastasis formation, and may influence cancer invasiveness. Specific-miRNAs expressed in endometrial cancer tissues may serve as diagnostic markers of the disease, prognostic biomarkers, or play an important part in oncological therapy We aimed to describe the role of miRNAs in endometrial cancer with special consideration of miRNA 205.

ARNetMiT R Package: association rules based gene co-expression networks of miRNA targets.

PubMed

Özgür Cingiz, M; Biricik, G; Diri, B

2017-03-31

miRNAs are key regulators that bind to target genes to suppress their gene expression level. The relations between miRNA-target genes enable users to derive co-expressed genes that may be involved in similar biological processes and functions in cells. We hypothesize that target genes of miRNAs are co-expressed, when they are regulated by multiple miRNAs. With the usage of these co-expressed genes, we can theoretically construct co-expression networks (GCNs) related to 152 diseases. In this study, we introduce ARNetMiT that utilize a hash based association rule algorithm in a novel way to infer the GCNs on miRNA-target genes data. We also present R package of ARNetMiT, which infers and visualizes GCNs of diseases that are selected by users. Our approach assumes miRNAs as transactions and target genes as their items. Support and confidence values are used to prune association rules on miRNA-target genes data to construct support based GCNs (sGCNs) along with support and confidence based GCNs (scGCNs). We use overlap analysis and the topological features for the performance analysis of GCNs. We also infer GCNs with popular GNI algorithms for comparison with the GCNs of ARNetMiT. Overlap analysis results show that ARNetMiT outperforms the compared GNI algorithms. We see that using high confidence values in scGCNs increase the ratio of the overlapped gene-gene interactions between the compared methods. According to the evaluation of the topological features of ARNetMiT based GCNs, the degrees of nodes have power-law distribution. The hub genes discovered by ARNetMiT based GCNs are consistent with the literature.

Identification of targets of miRNA-221 and miRNA-222 in fulvestrant-resistant breast cancer

PubMed Central

Liu, Pengfei; Sun, Manna; Jiang, Wenhua; Zhao, Jinkun; Liang, Chunyong; Zhang, Huilai

2016-01-01

The present study aimed to identify the differentially expressed genes (DEGs) regulated by microRNA (miRNA)-221 and miRNA-222 that are associated with the resistance of breast cancer to fulvestrant. The GSE19777 transcription profile was downloaded from the Gene Expression Omnibus database, and includes data from three samples of antisense miRNA-221-transfected fulvestrant-resistant MCF7-FR breast cancer cells, three samples of antisense miRNA-222-transfected fulvestrant-resistant MCF7-FR cells and three samples of control inhibitor (green fluorescent protein)-treated fulvestrant-resistant MCF7-FR cells. The linear models for microarray data package in R/Bioconductor was employed to screen for DEGs in the miRNA-transfected cells, and the pheatmap package in R was used to perform two-way clustering. Pathway enrichment was conducted using the Gene Set Enrichment Analysis tool. Furthermore, a miRNA-messenger (m) RNA regulatory network depicting interactions between miRNA-targeted upregulated DEGs was constructed and visualized using Cytoscape. In total, 492 and 404 DEGs were identified for the antisense miRNA-221-transfected MCF7-FR cells and the antisense miRNA-222-transfected MCF7-FR cells, respectively. Genes of the pentose phosphate pathway (PPP) were significantly enriched in the antisense miRNA-221-transfected MCF7-FR cells. In addition, components of the Wnt signaling pathway and cell adhesion molecules (CAMs) were significantly enriched in the antisense miRNA-222-transfected MCF7-FR cells. In the miRNA-mRNA regulatory network, miRNA-222 was demonstrated to target protocadherin 10 (PCDH10). The results of the present study suggested that the PPP and Wnt signaling pathways, as well as CAMs and PCDH10, may be associated with the resistance of breast cancer to fulvestrant. PMID:27895744

miRNA-216 and miRNA-499 target cyb561d2 in zebrafish in response to fipronil exposure.

PubMed

Zhou, Yongyong; Huang, Hannian; Zhang, Kai; Ding, Xianfeng; Jia, Longlue; Yu, Liang; Zhu, Guonian; Guo, Jiangfeng

2016-07-01

MicroRNA (miRNA) can regulate the expression of its target gene by mediating mRNA cleavage or by translational repression at a post-transcriptional level. Usually, one miRNA may regulate many genes as its targets, while one gene may also be targeted by many miRNAs. We previously demonstrated that cyb561d2, whose protein product is involved in cell defense, and chemical stress, is targeted by miR-155 in adult zebrafish (Danio rerio) when exposed to fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl) phenyl]-4-[(trifluoromethyl) sulphinyl]-1H-pyrazole-3-carbonitrile). Microcosm Targets prediction showed that the cyb561d2 gene is also highly possibly targeted by miR-194a, miR-216b, miR-429, and miR-499. These interactions need to be further validated experimentally. In this study, we evaluated the effects of fipronil on miR-194a, miR-216b, miR-429, miR-499 and cyb561d2 in zebrafish and investigated whether these four miRNAs could regulate the expression of cyb561d2 in both mRNA and protein levels. The expression of cyb561d2 was upregulated in both mRNA and protein level in a dose-dependent manner upon stimulation of fipronil, and miR-216b and miR-499 were downregulated concurrently, whereas there was no significant changes were observed in the expression level of miR-194a and miR-429. The dual luciferase report assay demonstrated that miR-216b and miR-499 interacted with cyb561d2 3'-untranslated regions (3'-UTR), miR-194a and miR-429 did not stimulate degradation of cyb561d2 mRNA. The expression of cyb561d2 was reduced in both mRNA and protein level when ZF4 cells were transfected with miR-499 mimic, whereas expression level of both mRNA and protein was increased when endogenous miR-499 was inhibited by transfection with miR-499 inhibitor. Likewise, the mRNA and protein level of cyb561d2 was affected by treatment with the mimics and the inhibitor of miR-216b. In contrast, when ZF4 cells were transfected with a mimic of miR-194a or miR-429, the expression of cyb561d2

Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines.

PubMed

Mundalil Vasu, Mahesh; Anitha, Ayyappan; Takahashi, Taro; Thanseem, Ismail; Iwata, Keiko; Asakawa, Tetsuya; Suzuki, Katsuaki

2016-01-01

Evidence suggests neuroprotective effects of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), on the developed neurons in the adult brain. In contrast, the drug may be deleterious to immature or undifferentiated neural cells, although the mechanism is unclear. Recent investigations have suggested that microRNAs (miRNA) may be critical for effectiveness of psychotropic drugs including SSRI. We investigated whether fluoxetine could modulate expressions of neurologically relevant miRNAs in two neuroblastoma SK-N-SH and SH-SY5Y cell lines. Initial screening results revealed that three (miR-489, miR-572 and miR-663a) and four (miR-320a, miR-489, miR-572 and miR-663a) miRNAs were up-regulated in SK-N-SH cells and SH-SY5Y cells, respectively, after 24 hours treatment of fluoxetine (1-25 μM). Cell viability was reduced according to the dose of fluoxetine. The upregulation of miR-572 and miR-663a was consistent in both the SH-SY5Y and SK-N-SH cells, confirmed by a larger scale culture condition. Our data is the first in vitro evidence that fluoxetine could increase the expression of miRNAs in undifferentiated neural cells, and that putative target genes of those miRNAs have been shown to be involved in fundamental neurodevelopmental processes.

Introduction of hsa-miR-103a and hsa-miR-1827 and hsa-miR-137 as new regulators of Wnt signaling pathway and their relation to colorectal carcinoma.

PubMed

Fasihi, Ali; M Soltani, Bahram; Atashi, Amir; Nasiri, Shirzad

2018-07-01

Wnt signaling is hyper-activated in most of human cancers including colorectal carcinoma (CRC). Therefore, the introduction of new regulators for Wnt pathway possesses promising diagnostic and therapeutic applications in cancer medicine. Bioinformatics analysis introduced hsa-miR-103a, hsa-miR-1827, and hsa-miR-137 as potential regulators of Wnt signaling pathway. Here, we intended to examine the effect of these human miRNAs on Wnt signaling pathway components, on the cell cycle progression in CRC originated cell lines and their expression in CRC tissues. RT-qPCR results indicated upregulation of hsa-miR-103a, hsa-miR-1827, and downregulation of hsa-miR-137 in CRC tissues. Overexpression of hsa-miR-103a and hsa-miR-1827 in SW480 cells resulted in elevated Wnt activity, detected by both Top/Flash assay and RT-qPCR analysis. Inhibition of Wnt signaling by using PNU-74654 or IWP-2 small molecules suggested that these miRNAs exerts their effect at the β-catenin degradation complex level. Then, RT-qPCR, dual luciferase assay, and western blotting analysis indicated that APC and APC2 transcripts were targeted by hsa-miR-103a, hsa-miR-1827 while, Wnt3a and β-catenin genes were upregulated. However, hsa-miR-137 downregulated Wnt3a and β-catenin genes. Further, hsa-miR-103a and hsa-miR-1827 overexpression resulted in cell cycle progression and reduced apoptotic rate in SW480 cells, unlike hsa-miR-137 overexpression which resulted in cell cycle suppression, detected by flowcytometry and Anexin analysis. Overall, our data introduced hsa-miR-103a, hsa-miR-1827 as onco-miRNAs and hsa-miR-137 as tumor suppressor which exert their effect through regulation of Wnt signaling pathway in CRC and introduced them as potential target for therapy. © 2017 Wiley Periodicals, Inc.

So, Why Sol-Mi? American Music Education

ERIC Educational Resources Information Center

Bennett, Peggy D.

2005-01-01

Walk into any primary grade music class in the U.S., and you will likely hear teacher and students singing a musical greeting, such as "Good morning boys and girls" (sol-mi-mi-sol-sol-mi) and the response "Good morning Miss Purdy" (sol-mi-mi-sol-mi-mi). Since about the 1970s, teachers have been beginning and ending music class for young children…

CID-miRNA: A web server for prediction of novel miRNA precursors in human genome

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tyagi, Sonika; Vaz, Candida; Gupta, Vipin

2008-08-08

microRNAs (miRNA) are a class of non-protein coding functional RNAs that are thought to regulate expression of target genes by direct interaction with mRNAs. miRNAs have been identified through both experimental and computational methods in a variety of eukaryotic organisms. Though these approaches have been partially successful, there is a need to develop more tools for detection of these RNAs as they are also thought to be present in abundance in many genomes. In this report we describe a tool and a web server, named CID-miRNA, for identification of miRNA precursors in a given DNA sequence, utilising secondary structure-based filteringmore » systems and an algorithm based on stochastic context free grammar trained on human miRNAs. CID-miRNA analyses a given sequence using a web interface, for presence of putative miRNA precursors and the generated output lists all the potential regions that can form miRNA-like structures. It can also scan large genomic sequences for the presence of potential miRNA precursors in its stand-alone form. The web server can be accessed at (http://mirna.jnu.ac.in/cidmirna/)« less

MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.

PubMed

James, Amanda Marie; Baker, Meredith B; Bao, Gang; Searles, Charles D

2017-01-01

MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro . The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples.

MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA

PubMed Central

James, Amanda Marie; Baker, Meredith B.; Bao, Gang; Searles, Charles D.

2017-01-01

MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro. The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples. PMID:28255356

Induced miR-99a expression represses Mtor cooperatively with miR-150 to promote regulatory T-cell differentiation

PubMed Central

Warth, Sebastian C; Hoefig, Kai P; Hiekel, Anian; Schallenberg, Sonja; Jovanovic, Ksenija; Klein, Ludger; Kretschmer, Karsten; Ansel, K Mark; Heissmeyer, Vigo

2015-01-01

Peripheral induction of regulatory T (Treg) cells provides essential protection from inappropriate immune responses. CD4+ T cells that lack endogenous miRNAs are impaired to differentiate into Treg cells, but the relevant miRNAs are unknown. We performed an overexpression screen with T-cell-expressed miRNAs in naive mouse CD4+ T cells undergoing Treg differentiation. Among 130 candidates, the screen identified 29 miRNAs with a negative and 10 miRNAs with a positive effect. Testing reciprocal Th17 differentiation revealed specific functions for miR-100, miR-99a and miR-10b, since all of these promoted the Treg and inhibited the Th17 program without impacting on viability, proliferation and activation. miR-99a cooperated with miR-150 to repress the expression of the Th17-promoting factor mTOR. The comparably low expression of miR-99a was strongly increased by the Treg cell inducer “retinoic acid”, and the abundantly expressed miR-150 could only repress Mtor in the presence of miR-99a. Our data suggest that induction of Treg cell differentiation is regulated by a miRNA network, which involves cooperation of constitutively expressed as well as inducible miRNAs. PMID:25712478

78 FR 50445 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-19

... School, 758 Eastern Ave. NE., Grand Rapids, 13000666 Lexington School, 45 Lexington, NW., Grand Rapids... Fremont High School, 204 E. Main, Fremont, 13000669 Oakland County Lower Trout Lake Bathhouse Complex and... Residential Historic District, Bounded by A, South, 56th & 70th Sts., Lincoln, 13000675 Sheldon Memorial Art...

Alpha-1 antitrypsin augmentation therapy decreases miR-199a-5p, miR-598 and miR-320a expression in monocytes via inhibition of NFκB.

PubMed

Hassan, Tidi; de Santi, Chiara; Mooney, Catherine; McElvaney, Noel G; Greene, Catherine M

2017-10-23

Alpha-1 antitrypsin (AAT) augmentation therapy involves infusion of plasma-purified AAT to AAT deficient individuals. Whether treatment affects microRNA expression has not been investigated. This study's objectives were to evaluate the effect of AAT augmentation therapy on altered miRNA expression in monocytes and investigate the mechanism. Monocytes were isolated from non-AAT deficient (MM) and AAT deficient (ZZ) individuals, and ZZs receiving AAT. mRNA (qRT-PCR, microarray), miRNA (miRNA profiling, qRT-PCR), and protein (western blotting) analyses were performed. Twenty one miRNAs were differentially expressed 3-fold between ZZs and MMs. miRNA validation studies demonstrated that in ZZ monocytes receiving AAT levels of miR-199a-5p, miR-598 and miR-320a, which are predicted to be regulated by NFκB, were restored to levels similar to MMs. Validated targets co-regulated by these miRNAs were reciprocally increased in ZZs receiving AAT in vivo and in vitro. Expression of these miRNAs could be increased in ZZ monocytes treated ex vivo with an NFκB agonist and decreased by NFκB inhibition. p50 and p65 mRNA and protein were significantly lower in ZZs receiving AAT than untreated ZZs. AAT augmentation therapy inhibits NFκB and decreases miR-199a-5p, miR-598 and miR-320a in ZZ monocytes. These NFκB-inhibitory properties may contribute to the anti-inflammatory effects of AAT augmentation therapy.

Downregulation of miR-133 and miR-590 contributes to nicotine-induced atrial remodelling in canines.

PubMed

Shan, Hongli; Zhang, Yong; Lu, Yanjie; Zhang, Ying; Pan, Zhenwei; Cai, Benzhi; Wang, Ning; Li, Xuelian; Feng, Tieming; Hong, Yuan; Yang, Baofeng

2009-08-01

The present study was designed to decipher molecular mechanisms underlying nicotine's promoting atrial fibrillation (AF) by inducing atrial structural remodelling. The canine model of AF was successfully established by nicotine administration and rapid pacing. The atrial fibroblasts isolated from healthy dogs were treated with nicotine. The role of microRNAs (miRNAs) on the expression and regulation of transforming growth factor-beta1 (TGF-beta1), TGF-beta receptor type II (TGF-betaRII), and collagen production was evaluated in vivo and in vitro. Administration of nicotine for 30 days increased AF vulnerability by approximately eight- to 15-fold in dogs. Nicotine stimulated remarkable collagen production and atrial fibrosis both in vitro in cultured canine atrial fibroblasts and in vivo in atrial tissues. Nicotine produced significant upregulation of expression of TGF-beta1 and TGF-betaRII at the protein level, and a 60-70% decrease in the levels of miRNAs miR-133 and miR-590. This downregulation of miR-133 and miR-590 partly accounts for the upregulation of TGF-beta1 and TGF-betaRII, because our data established TGF-beta1 and TGF-betaRII as targets for miR-133 and miR-590 repression. Transfection of miR-133 or miR-590 into cultured atrial fibroblasts decreased TGF-beta1 and TGF-betaRII levels and collagen content. These effects were abolished by the antisense oligonucleotides against miR-133 or miR-590. The effects of nicotine were prevented by an alpha7 nicotinic acetylcholine receptor antagonist. We conclude that the profibrotic response to nicotine in canine atrium is critically dependent upon downregulation of miR-133 and miR-590.

Trends in maternal and infant health in poor urban neighborhoods: good news from the 1990s, but challenges remain.

PubMed

Howell, Embry M; Pettit, Kathryn L S; Kingsley, G Thomas

2005-01-01

During the 1990s, numerous public policy changes occurred that may have affected the health of mothers and infants in low-income neighborhoods. This article examines trends in key maternal and child health indicators to determine whether disparities between high-poverty neighborhoods and other neighborhoods have declined. Using neighborhood-level vital statistics and U.S. Census data, we categorized "neighborhoods" (Census tracts) as being high poverty (greater than 30% of population below the federal poverty level in 1990) or not. We compared trends in four key indicators--births to teenagers, late prenatal care, low birth-weight; and infant mortality--over the 1990s among high-poverty and other neighborhoods in Cuyahoga County, Ohio; Denver, Colorado; Marion County, Indiana; and Oakland, California. In all four metropolitan areas, trends in high-poverty neighborhoods were more favorable than in other neighborhoods. The most consistently positive trend was the reduction in the rate of teen births. The metropolitan areas with the most intensive programs to improve maternal and child health--Cuyahoga County and Oakland-saw the most consistent improvement across all indicators. Still, great disparities between high-poverty and other neighborhoods remain, and only Oakland shows promise of achieving some of the Healthy People 2010 maternal and child health goals in its high-poverty neighborhoods. While there has been a reduction in maternal and infant health disparities between high-poverty and other neighborhoods, much work remains to eliminate disparities and achieve the 2010 goals. Small area data are useful in isolating the neighborhoods that should be targeted. Experience from the 1990s suggests that a combination of several intensive interventions can be effective at reducing disparities.

miR-200b and miR-200c as prognostic factors and mediators of gastric cancer cell progression.

PubMed

Tang, Hailin; Deng, Min; Tang, Yunyun; Xie, Xinhua; Guo, Jiaoli; Kong, Yanan; Ye, Feng; Su, Qi; Xie, Xiaoming

2013-10-15

The purpose of this study was to investigate the clinicopathologic significance and potential role of miR-200b and miR-200c in the development and progression of gastric cancer. We examined miR-200b and miR-200c expression in 36 paired normal and stomach tumor specimens, as well as gastric cancer cell lines, by quantitative real-time PCR. In addition, miR-200b and miR-200c were detected by ISH using gastric cancer tissue microarrays, and the association between miR-200b and miR-200c levels and clinicopathologic factors and prognosis were analyzed. A luciferase assay was conducted for target evaluation. The functional effects of miR-200b and miR-200c on gastric cancer cells were validated by a cell proliferation assay and cell invasion and migration assays. miR-200b and miR-200c were downregulated in the gastric cancer specimens and cell lines tested. miR-200b and miR-200c levels were significantly correlated with the clinical stage, T stage, lymph node metastasis, and survival of patients. Ectopic expression of miR-200b and miR-200c impaired cell growth and invasion. In addition, when overexpressed, miR-200b and miR-200c commonly directly targeted DNMT3A, DNMT3B, and SP1 (a transactivator of the DNMT1 gene), which resulted in marked reduction of the expression of DNA methyltransferases DNMT1, DNMT3A, and DNMT3B at the protein level. This effect, in turn, led to a decrease in global DNA methylation and reexpression of p16, RASS1A1, and E-cadherin via promoter DNA hypomethylation. Our findings suggest that miR-200b and miR-200c, as valuable markers of gastric cancer prognosis, may be a promising approach to human gastric cancer treatment. ©2013 AACR.

Role of miR-383 and miR-146b in different propensities to obesity in male mice.

PubMed

Xia, Shu-Fang; Duan, Xiao-Mei; Cheng, Xiang-Rong; Chen, Li-Mei; Kang, Yan-Jun; Wang, Peng; Tang, Xue; Shi, Yong-Hui; Le, Guo-Wei

2017-08-01

The study was designed to investigate the possible mechanisms of hepatic microRNAs (miRs) in regulating local thyroid hormone (TH) action and ultimately different propensities to high-fat diet (HFD)-induced obesity. When obesity-prone (OP) and obesity-resistant (OR) mice were fed HFD for 7 weeks, OP mice showed apparent hepatic steatosis, with significantly higher body weight and lower hepatic TH receptor b (TRb) expression and type 1 deiodinase (DIO1) activity than OR mice. Next-generation sequencing technology revealed that 13 miRs in liver were dysregulated between the two phenotypes, of which 8 miRs were predicted to target on Dio1 or TRb When mice were fed for 17 weeks, OR mice had mild hepatic steatosis and increased Dio1 and TRb expression than OP mice, with downregulation of T3 target genes (including Srebp1c , Acc1 , Scd1 and Fasn ) and upregulation of Cpt1α , Atp5c1 , Cox7c and Cyp7a1 A stem-loop qRT-PCR analysis confirmed that the levels of miR-383, miR-34a and miR-146b were inversely correlated with those of DIO1 or TRb. Down-regulated expression of miR-383 or miR-146b by miR-383 inhibitor (anti-miR-383) or miR-146b inhibitor (anti-miR-146b) in free fatty acid-treated primary mouse hepatocytes led to increased DIO1 and TRb expressions, respectively, and subsequently decreased cellular lipid accumulation, while miR-34a inhibitor (anti-miR-34a) transfection had on effects on TRb expression. Luciferase reporter assay illustrated that miR-146b could directly target TRb 3'untranslated region (3'UTR). These findings suggested that miR-383 and miR-146b might play critical roles in different propensities to diet-induced obesity via targeting on Dio1 and TRb , respectively. © 2017 Society for Endocrinology.

miRNA 206 and miRNA 574-5p are highly expression in coronary artery disease

PubMed Central

Zhou, Jianqing; Shao, Guofeng; Chen, Xiaoliang; Yang, Xi; Huang, Xiaoyan; Peng, Ping; Ba, Yanna; Zhang, Lin; Jehangir, Tashina; Bu, Shizhong; Liu, Ningsheng; Lian, Jiangfang

2015-01-01

Coronary artery disease (CAD) is the leading cause of human morbidity and mortality worldwide. Innovative diagnostic biomarkers are a pressing need for this disease. miRNAs profiling is an innovative method of identifying biomarkers for many diseases and could be proven as a powerful tool in the diagnosis and treatment of CAD. We performed miRNA microarray analysis from the plasma of three CAD patients and three healthy controls. Subsequently, we performed quantitative real-time PCR (qRT-PCR) analysis of miRNA expression in plasma of another 67 CAD patients and 67 healthy controls. We identified two miRNAs (miR-206 and miR-574-5p) that were significantly up-regulated in CAD patients as compared with healthy controls (P<0.05). The receiver operating characteristic (ROC) curves indicated these two miRNAs had great potential to provide sensitive and specific diagnostic value for CAD. PMID:26685009

miRWalk--database: prediction of possible miRNA binding sites by "walking" the genes of three genomes.

PubMed

Dweep, Harsh; Sticht, Carsten; Pandey, Priyanka; Gretz, Norbert

2011-10-01

MicroRNAs are small, non-coding RNA molecules that can complementarily bind to the mRNA 3'-UTR region to regulate the gene expression by transcriptional repression or induction of mRNA degradation. Increasing evidence suggests a new mechanism by which miRNAs may regulate target gene expression by binding in promoter and amino acid coding regions. Most of the existing databases on miRNAs are restricted to mRNA 3'-UTR region. To address this issue, we present miRWalk, a comprehensive database on miRNAs, which hosts predicted as well as validated miRNA binding sites, information on all known genes of human, mouse and rat. All mRNAs, mitochondrial genes and 10 kb upstream flanking regions of all known genes of human, mouse and rat were analyzed by using a newly developed algorithm named 'miRWalk' as well as with eight already established programs for putative miRNA binding sites. An automated and extensive text-mining search was performed on PubMed database to extract validated information on miRNAs. Combined information was put into a MySQL database. miRWalk presents predicted and validated information on miRNA-target interaction. Such a resource enables researchers to validate new targets of miRNA not only on 3'-UTR, but also on the other regions of all known genes. The 'Validated Target module' is updated every month and the 'Predicted Target module' is updated every 6 months. miRWalk is freely available at http://mirwalk.uni-hd.de/. Copyright © 2011 Elsevier Inc. All rights reserved.

Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals

PubMed Central

Atanasov, Janina; Groher, Florian

2017-01-01

Abstract Synthetic RNA-based systems have increasingly been used for the regulation of eukaryotic gene expression. Due to their structural properties, riboregulators provide a convenient basis for the development of ligand-dependent controllable systems. Here, we demonstrate reversible conditional control of miRNA biogenesis with an aptamer domain as a sensing unit connected to a natural miRNA precursor for the first time. For the design of the pre-miR switch, we replaced the natural terminal loop with the TetR aptamer. Thus, the TetR aptamer was positioned close to the Dicer cleavage sites, which allowed sterical control over pre-miR processing by Dicer. Our design proved to be highly versatile, allowing us to regulate the biogenesis of three structurally different miRNAs: miR-126, -34a and -199a. Dicer cleavage was inhibited up to 143-fold via co-expression of the TetR protein, yet could be completely restored upon addition of doxycycline. Moreover, we showed the functionality of the pre-miR switches for gene regulation through the interaction of the respective miRNA with its specific target sequence. Our designed device is capable of robust and reversible control of miRNA abundance. Thus, we offer a novel investigational tool for functional miRNA analysis. PMID:29036355

Downregulation of miR-99a/let-7c/miR-125b miRNA cluster predicts clinical outcome in patients with unresected malignant pleural mesothelioma

PubMed Central

Genova, Carlo; Mora, Marco; Dal Bello, Maria Giovanna; Vanni, Irene; Alama, Angela; Rijavec, Erika; Biello, Federica; Barletta, Giulia; Merlo, Domenico Franco; Valentino, Alessandro; Ferro, Paola; Ravetti, Gian Luigi; Stigliani, Sara; Vigani, Antonella; Fedeli, Franco; Beer, David G.; Roncella, Silvio; Grossi, Francesco

2017-01-01

Malignant pleural mesothelioma (MPM) is an aggressive tumor with a dismal overall survival (OS) and to date no molecular markers are available to guide patient management. This study aimed to identify a prognostic miRNA signature in MPM patients who did not undergo tumor resection. Whole miRNA profiling using a microarray platform was performed using biopsies on 27 unresected MPM patients with distinct clinical outcome: 15 patients had short survival (OS<12 months) and 12 patients had long survival (OS>36 months). Three prognostic miRNAs (mir-99a, let-7c, and miR-125b) encoded at the same cluster (21q21) were selected for further validation and tested on publicly available miRNA sequencing data from 72 MPM patients with survival data. A risk model was built based on these 3 miRNAs that was validated by quantitative PCR in an independent set of 30 MPM patients. High-risk patients had shorter median OS (7.6 months) as compared with low-risk patients (median not reached). In the multivariate Cox model, a high-risk score was independently associated with shorter OS (HR=3.14; 95% CI, 1.18–8.34; P=0.022). Our study identified that the downregulation of the miR-99a/let-7/miR-125b miRNA cluster predicts poor outcome in unresected MPM. PMID:28978143

Circulating microRNA miR-21-5p, miR-150-5p and miR-30e-5p correlate with clinical status in late onset myasthenia gravis.

PubMed

Sabre, Liis; Maddison, Paul; Sadalage, Girija; Ambrose, Philip Alexander; Punga, Anna Rostedt

2018-05-08

There are no biomarkers for late onset myasthenia gravis (LOMG; onset >50 years). We evaluated circulating microRNA in a discovery cohort of 4 LOMG patients and 4 healthy controls and in a prospective diagnostic validation cohort of 73 LOMG patients (48 male) with longitudinal follow-up samples. In immunosuppression naïve patients, levels of miRNAs miR-150-5p, miR-21-5p and miR-30e-5p decreased in parallel with clinical improvement after initiation of immunosuppression and their levels positively correlated with the clinical MG composite score. Levels of miR-150-5p and miR-21-5p were lower in patients with ocular compared to generalized LOMG. Circulating miR-150-5p, miR-21-5p and miR-30e-5p correlate with the clinical course in LOMG. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Web-based NGS data analysis using miRMaster: a large-scale meta-analysis of human miRNAs.

PubMed

Fehlmann, Tobias; Backes, Christina; Kahraman, Mustafa; Haas, Jan; Ludwig, Nicole; Posch, Andreas E; Würstle, Maximilian L; Hübenthal, Matthias; Franke, Andre; Meder, Benjamin; Meese, Eckart; Keller, Andreas

2017-09-06

The analysis of small RNA NGS data together with the discovery of new small RNAs is among the foremost challenges in life science. For the analysis of raw high-throughput sequencing data we implemented the fast, accurate and comprehensive web-based tool miRMaster. Our toolbox provides a wide range of modules for quantification of miRNAs and other non-coding RNAs, discovering new miRNAs, isomiRs, mutations, exogenous RNAs and motifs. Use-cases comprising hundreds of samples are processed in less than 5 h with an accuracy of 99.4%. An integrative analysis of small RNAs from 1836 data sets (20 billion reads) indicated that context-specific miRNAs (e.g. miRNAs present only in one or few different tissues / cell types) still remain to be discovered while broadly expressed miRNAs appear to be largely known. In total, our analysis of known and novel miRNAs indicated nearly 22 000 candidates of precursors with one or two mature forms. Based on these, we designed a custom microarray comprising 11 872 potential mature miRNAs to assess the quality of our prediction. MiRMaster is a convenient-to-use tool for the comprehensive and fast analysis of miRNA NGS data. In addition, our predicted miRNA candidates provided as custom array will allow researchers to perform in depth validation of candidates interesting to them. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

MiR-126 and miR-126* regulate shear-resistant firm leukocyte adhesion to human brain endothelium

PubMed Central

Cerutti, Camilla; Edwards, Laura J.; de Vries, Helga E.; Sharrack, Basil; Male, David K.; Romero, Ignacio A.

2017-01-01

Leukocyte adhesion to brain endothelial cells, the blood-brain barrier main component, is a critical step in the pathogenesis of neuroinflammatory diseases such as multiple sclerosis (MS). Leukocyte adhesion is mediated mainly by selectins, cell adhesion molecules and chemokines induced by pro-inflammatory cytokines such as TNFα and IFNγ, but the regulation of this process is not fully clear. This study investigated the regulation of firm leukocyte adhesion to human brain endothelium by two different brain endothelial microRNAs (miRs), miR-126 and miR-126*, that are downregulated by TNFα and IFNγ in a human brain endothelial cell line, hCMEC/D3. Using a leukocyte adhesion in vitro assay under shear forces mimicking blood flow, we observed that reduction of endothelial miR-126 and miR-126* enhanced firm monocyte and T cell adhesion to hCMEC/D3 cells, whereas their increased expression partially prevented THP1, Jurkat and primary MS patient-derived PBMC firm adhesion. Furthermore, we observed that miR-126* and miR-126 downregulation increased E-selectin and VCAM1, respectively, while miR-126 overexpression reduced VCAM1 and CCL2 expression by hCMEC/D3 cells, suggesting that these miRs regulate leukocyte adhesion by modulating the expression of adhesion-associated endothelial mRNA targets. Hence, human brain endothelial miR-126 and miR-126* could be used as a therapeutic tool to reduce leukocyte adhesion and thus reduce neuroinflammation. PMID:28358058

Streamflow, water quality, and aquatic macroinvertebrates of selected streams in Fairfax County, Virginia, 2007-12

USGS Publications Warehouse

Jastram, John D.

2014-01-01

few exceptions. Nitrogen concentrations in monthly samples were generally low and dominated by nitrate. Exceptions to the generally low N concentrations occurred at Captain Hickory Run, which had a median total N concentration of approximately 4.9 milligrams per liter (mg/L), compared to the network-wide median of approximately 1.7 mg/L, and at Popes Head Creek Tributary, where total N concentrations spiked to 6–8 mg/L during low-flow periods in August or September of each year. Phosphorus concentrations in monthly samples were generally low and dominated by the dissolved fraction. Two monitoring stations in the network, Flatlick Branch and Frog Branch, are notable for having median total P concentrations that were, on average, approximately three times greater than the median total P concentration of 0.02 mg/L observed at the other 12 stations in the network. Suspended-sediment and nutrient loads and yields were similar to those of urbanized watersheds in other studies, although the yields from these urbanized basins were greater than, or within, the upper quartile of yields observed throughout the Chesapeake Bay watershed. Annual suspended-sediment loads ranged from 289–10,275 tons, with a median of 1,311 tons, and corresponding yields ranged from 107–2,827 tons per square mile (ton/mi2), with a median of 277 ton/mi2. Annual total N loads ranged from 8,014–36,413 pounds, with a median of 21,314 pounds, and corresponding yields ranged from 3,361–8,360 pounds per square mile (lb/mi2), with a median of 6,200 lb/mi2. Annual total P loads ranged from 380–6,558 pounds, with a median of 1,874 pounds, and corresponding yields ranged from 140–1,562 lb/mi2, with a median of 543 lb/mi2. Benthic macroinvertebrate community metrics indicated that streams throughout Fairfax County are generally of poor health. One station, Castle Creek, was an exception with results indicating relatively high quality aquatic health. Six additional monitoring stations were added to

miRNA Signatures of Insulin Resistance in Obesity.

PubMed

Jones, Angela; Danielson, Kirsty M; Benton, Miles C; Ziegler, Olivia; Shah, Ravi; Stubbs, Richard S; Das, Saumya; Macartney-Coxson, Donia

2017-10-01

Extracellular microRNAs (miRNAs) represent functional biomarkers for obesity and related disorders; this study investigated plasma miRNAs in insulin resistance phenotypes in obesity. One hundred seventy-five miRNAs were analyzed in females with obesity (insulin sensitivity, n = 11; insulin resistance, n = 19; type 2 diabetes, n = 15) and without obesity (n = 12). Correlations between miRNA level and clinical parameters and levels of 15 miRNAs in a murine obesity model were investigated. One hundred six miRNAs were significantly (adjusted P ≤ 0.05) different between controls and at least one obesity phenotype, including miRNAs with the following attributes: previously reported roles in obesity and altered circulating levels (e.g., miR-122, miR-192); known roles in obesity but no reported changes in circulating levels (e.g., miR-378a); and no current reported role in, or association with, obesity (e.g., miR-28-5p, miR-374b, miR-32). The miRNAs in the latter group were found to be associated with extracellular vesicles. Forty-eight miRNAs showed significant correlations with clinical parameters; stepwise regression retained let-7b, miR-144-5p, miR-34a, and miR-532-5p in a model predictive of insulin resistance (R 2  = 0.57, P = 7.5 × 10 -8 ). Both miR-378a and miR-122 were perturbed in metabolically relevant tissues in a murine model of obesity. This study expands on the role of extracellular miRNAs in insulin-resistant phenotypes of obesity and identifies candidate miRNAs not previously associated with obesity. © 2017 The Obesity Society.

Bone-related Circulating MicroRNAs miR-29b-3p, miR-550a-3p, and miR-324-3p and their Association to Bone Microstructure and Histomorphometry.

PubMed

Feichtinger, Xaver; Muschitz, Christian; Heimel, Patrick; Baierl, Andreas; Fahrleitner-Pammer, Astrid; Redl, Heinz; Resch, Heinrich; Geiger, Elisabeth; Skalicky, Susanna; Dormann, Rainer; Plachel, Fabian; Pietschmann, Peter; Grillari, Johannes; Hackl, Matthias; Kocijan, Roland

2018-03-20

The assessment of bone quality and the prediction of fracture risk in idiopathic osteoporosis (IOP) are complex prospects as bone mineral density (BMD) and bone turnover markers (BTM) do not indicate fracture-risk. MicroRNAs (miRNAs) are promising new biomarkers for bone diseases, but the current understanding of the biological information contained in the variability of miRNAs is limited. Here, we investigated the association between serum-levels of 19 miRNA biomarkers of idiopathic osteoporosis to bone microstructure and bone histomorphometry based upon bone biopsies and µCT (9.3 μm) scans from 36 patients. Four miRNAs were found to be correlated to bone microarchitecture and seven miRNAs to dynamic histomorphometry (p < 0.05). Three miRNAs, namely, miR-29b-3p, miR-324-3p, and miR-550a-3p showed significant correlations to histomorphometric parameters of bone formation as well as microstructure parameters. miR-29b-3p and miR-324-p were found to be reduced in patients undergoing anti-resorptive therapy. This is the first study to report that serum levels of bone-related miRNAs might be surrogates of dynamic histomorphometry and potentially reveal changes in bone microstructure. Although these findings enhance the potential value of circulating miRNAs as bone biomarkers, further experimental studies are required to qualify the clinical utility of miRNAs to reflect dynamic changes in bone formation and microstructure.

A Values-Based Motivational Interviewing (MI) Intervention for Pediatric Obesity: Study Design and Methods for MI Values

PubMed Central

Bean, Melanie K.; Mazzeo, Suzanne E.; Stern, Marilyn; Bowen, Deborah; Ingersoll, Karen

2011-01-01

To reduce pediatric obesity in clinical settings, multidisciplinary behaviorally-based treatment programs are recommended. High attrition and poor compliance are two difficulties frequently encountered in such programs. A brief, empathic and directive clinical intervention, Motivational Interviewing (MI), might help address these motivational and behavioral issues, ultimately resulting in more positive health outcomes. The efficacy of MI as an adjunct in the treatment of pediatric obesity remains relatively understudied. MI Values was developed to implement within an existing multidisciplinary treatment program for obese, ethnically diverse adolescents, the T.E.E.N.S. Program (Teaching, Encouragement, Exercise, Nutrition, Support). T.E.E.N.S. participants who consent to MI Values are randomized to either MI or an education control condition. At weeks 1 and 10 of T.E.E.N.S. participation, the subset of participants assigned to the MI condition engage in individual MI sessions and control participants view health education videos. All MI sessions are audiotaped and coded to monitor treatment fidelity, which has been satisfactory thus far. Participants complete comprehensive assessments at baseline, 3-and 6-month follow-up. We hypothesize that MI participants will demonstrate greater reductions in Body Mass Index (BMI) percentile, improved diet and physical activity behaviors, better compliance with T.E.E.N.S., and lower attrition than participants in the control group. We present study design and methods for MI Values as well as data on feasibility of recruitment methods and treatment integrity. At study completion, findings will contribute to the emerging literature examining the efficacy of MI in the treatment of pediatric obesity. PMID:21554994

Characterization of the mammalian miRNA turnover landscape

PubMed Central

Guo, Yanwen; Liu, Jun; Elfenbein, Sarah J.; Ma, Yinghong; Zhong, Mei; Qiu, Caihong; Ding, Ye; Lu, Jun

2015-01-01

Steady state cellular microRNA (miRNA) levels represent the balance between miRNA biogenesis and turnover. The kinetics and sequence determinants of mammalian miRNA turnover during and after miRNA maturation are not fully understood. Through a large-scale study on mammalian miRNA turnover, we report the co-existence of multiple cellular miRNA pools with distinct turnover kinetics and biogenesis properties and reveal previously unrecognized sequence features for fast turnover miRNAs. We measured miRNA turnover rates in eight mammalian cell types with a combination of expression profiling and deep sequencing. While most miRNAs are stable, a subset of miRNAs, mostly miRNA*s, turnovers quickly, many of which display a two-step turnover kinetics. Moreover, different sequence isoforms of the same miRNA can possess vastly different turnover rates. Fast turnover miRNA isoforms are enriched for 5′ nucleotide bias against Argonaute-(AGO)-loading, but also additional 3′ and central sequence features. Modeling based on two fast turnover miRNA*s miR-222-5p and miR-125b-1-3p, we unexpectedly found that while both miRNA*s are associated with AGO, they strongly differ in HSP90 association and sensitivity to HSP90 inhibition. Our data characterize the landscape of genome-wide miRNA turnover in cultured mammalian cells and reveal differential HSP90 requirements for different miRNA*s. Our findings also implicate rules for designing stable small RNAs, such as siRNAs. PMID:25653157

miRvestigator: web application to identify miRNAs responsible for co-regulated gene expression patterns discovered through transcriptome profiling.

PubMed

Plaisier, Christopher L; Bare, J Christopher; Baliga, Nitin S

2011-07-01

Transcriptome profiling studies have produced staggering numbers of gene co-expression signatures for a variety of biological systems. A significant fraction of these signatures will be partially or fully explained by miRNA-mediated targeted transcript degradation. miRvestigator takes as input lists of co-expressed genes from Caenorhabditis elegans, Drosophila melanogaster, G. gallus, Homo sapiens, Mus musculus or Rattus norvegicus and identifies the specific miRNAs that are likely to bind to 3' un-translated region (UTR) sequences to mediate the observed co-regulation. The novelty of our approach is the miRvestigator hidden Markov model (HMM) algorithm which systematically computes a similarity P-value for each unique miRNA seed sequence from the miRNA database miRBase to an overrepresented sequence motif identified within the 3'-UTR of the query genes. We have made this miRNA discovery tool accessible to the community by integrating our HMM algorithm with a proven algorithm for de novo discovery of miRNA seed sequences and wrapping these algorithms into a user-friendly interface. Additionally, the miRvestigator web server also produces a list of putative miRNA binding sites within 3'-UTRs of the query transcripts to facilitate the design of validation experiments. The miRvestigator is freely available at http://mirvestigator.systemsbiology.net.

miR-137 and miR-491 Negatively Regulate Dopamine Transporter Expression and Function in Neural Cells.

PubMed

Jia, Xiaojian; Wang, Feng; Han, Ying; Geng, Xuewen; Li, Minghua; Shi, Yu; Lu, Lin; Chen, Yun

2016-12-01

The dopamine transporter (DAT) is involved in the regulation of extracellular dopamine levels. A 40-bp variable-number tandem repeat (VNTR) polymorphism in the 3'-untranslated region (3'UTR) of the DAT has been reported to be associated with various phenotypes that are involved in the aberrant regulation of dopaminergic neurotransmission. In the present study, we found that miR-137 and miR-491 caused a marked reduction of DAT expression, thereby influencing neuronal dopamine transport. Moreover, the regulation of miR-137 and miR-491 on this transport disappeared after the DAT was silenced. The miR-491 seed region that is located on the VNTR sequence in the 3'UTR of the DAT and the regulatory effect of miR-491 on the DAT depended on the VNTR copy-number. These data indicate that miR-137 and miR-491 regulate DAT expression and dopamine transport at the post-transcriptional level, suggesting that microRNA may be targeted for the treatment of diseases associated with DAT dysfunction.

MiRNAs in bone diseases.

PubMed

Moore, Benjamin T; Xiao, Peng

2013-01-01

MicroRNAs (miRNAs), which mainly inhibit protein expression by targeting the 3'UTR (untranslated region) of mRNAs, are known to play various roles in the pathogenesis of many different types of diseases. Specifically, in bone diseases, recent emphasis has been placed on the involvement of miRNAs in the differentiation and proliferation of bone and cartilage cells, particularly with regards to how these mechanisms contribute to bone homeostasis. In this review, we summarize miRNAs that are important in the differentiation and proliferation of bone cells, and specific miRNAs associated with bone diseases, such as osteoporosis, osteoarthritis and rheumatoid arthritis. This review also provides the perspective that miRNA studies will identify not only new mechanisms in basic bone research, but also potential novel diagnostic biomarkers and drug targets for bone diseases.

Shallow-depth location and geometry of the Piedmont Reverse splay of the Hayward Fault, Oakland, California

USGS Publications Warehouse

Catchings, Rufus D.; Goldman, Mark R.; Trench, David; Buga, Michael; Chan, Joanne H.; Criley, Coyn J.; Strayer, Luther M.

2017-04-18

The Piedmont Thrust Fault, herein referred to as the Piedmont Reverse Fault (PRF), is a splay of the Hayward Fault that trends through a highly populated area of the City of Oakland, California (fig. 1A). Although the PRF is unlikely to generate a large-magnitude earthquake, slip on the PRF or high-amplitude seismic energy traveling along the PRF may cause considerable damage during a large earthquake on the Hayward Fault. Thus, it is important to determine the exact location, geometry (particularly dip), and lateral extent of the PRF within the densely populated Oakland area. In the near surface, the PRF juxtaposes Late Cretaceous sandstone (of the Franciscan Complex Novato Quarry terrane of Blake and others, 1984) and an older Pleistocene alluvial fan unit along much of its mapped length (fig. 1B; Graymer and others, 1995). The strata of the Novato Quarry unit vary greatly in strike (NW, NE, and E), dip direction (NE, SW, E, and NW), dip angle (15° to 85°), and lithology (shale and sandstone), and the unit has been intruded by quartz diorite in places. Thus, it is difficult to infer the structure of the fault, particularly at depth, with conventional seismic reflection imaging methods. To better determine the location and shallow-depth geometry of the PRF, we used high-resolution seismic imaging methods described by Catchings and others (2014). These methods involve the use of coincident P-wave (compressional wave) and S-wave (shear wave) refraction tomography and reflection data, from which tomographic models of P- and S-wave velocity and P-wave reflection images are developed. In addition, the coincident P-wave velocity (VP) and S-wave velocity (VS) data are used to develop tomographic models of VP/VS ratios and Poisson’s ratio, which are sensitive to shallow-depth faulting and groundwater. In this study, we also compare measurements of Swave velocities determined from surface waves with those determined from refraction tomography. We use the combination of

Identification of cancer-related miRNA-lncRNA biomarkers using a basic miRNA-lncRNA network.

PubMed

Zhang, Guangle; Pian, Cong; Chen, Zhi; Zhang, Jin; Xu, Mingmin; Zhang, Liangyun; Chen, Yuanyuan

2018-01-01

LncRNAs are regulatory noncoding RNAs that play crucial roles in many biological processes. The dysregulation of lncRNA is thought to be involved in many complex diseases; lncRNAs are often the targets of miRNAs in the indirect regulation of gene expression. Numerous studies have indicated that miRNA-lncRNA interactions are closely related to the occurrence and development of cancers. Thus, it is important to develop an effective method for the identification of cancer-related miRNA-lncRNA interactions. In this study, we compiled 155653 experimentally validated and predicted miRNA-lncRNA associations, which we defined as basic interactions. We next constructed an individual-specific miRNA-lncRNA network (ISMLN) for each cancer sample and a basic miRNA-lncRNA network (BMLN) for each type of cancer by examining the expression profiles of miRNAs and lncRNAs in the TCGA (The Cancer Genome Atlas) database. We then selected potential miRNA-lncRNA biomarkers based on the BLMN. Using this method, we identified cancer-related miRNA-lncRNA biomarkers and modules specific to a certain cancer. This method of profiling will contribute to the diagnosis and treatment of cancers at the level of gene regulatory networks.

Proteomics for understanding miRNA biology

PubMed Central

Huang, Tai-Chung; Pinto, Sneha M.; Pandey, Akhilesh

2013-01-01

MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology. PMID:23125164

Serum miR-29a and miR-122 as Potential Biomarkers for Non-Alcoholic Fatty Liver Disease (NAFLD).

PubMed

Jampoka, Kanisa; Muangpaisarn, Puth; Khongnomnan, Kritsada; Treeprasertsuk, Sombat; Tangkijvanich, Pisit; Payungporn, Sunchai

2018-05-30

Non-alcoholic fatty liver disease (NAFLD) is an over accumulation of triglyceride in the liver without alcohol consumption which its major cause is from insulin resistance. Patients with NAFLD can develop to be liver fibrosis, cirrhosis and hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) are non-coding RNAs that regulate post-transcriptional gene silencing. Previous research reported that miR-29 family (a, b and c) and miR-122 have an important role in regulating insulin resistance related to NAFLD. The purpose of this study was to investigate that miR-29 and miR-122 can be possible biomarkers for non-invasive diagnosis of NAFLD. Serum samples were collected from 58 NAFLD patients and 34 healthy controls. MiRNAs were extracted from serum by using microRNA purification kit followed by polyuridylation, reverse transcription and quantitative real-time PCR. Also, we analyzed the correlation between miR-29 and miR-122 and level of liver inflammation in NAFLD patients. We found that the serum miR-29a levels in NAFLD patients were significantly lower (P = 0.006) than the control group, while miR-29c levels were unchanged, and miR-29b levels were undetectable. However, we found that serum miR-122 levels in NAFLD patients were significantly higher (P < 0.001) than those found in the control group. For miR-29a, the area under curve (AUC) was 0.679 (P = 0.0065) with 60.87% sensitivity and 82.35% specificity. For miR-122, the AUC was 0.831 (P < 0.0001) with 75.00% sensitivity and 82.35% specificity. Interestingly, the level of serum miR-122 were significantly different between patients with not steatohepatitis (NAS < 4) and steatohepatitis (NAS ≥ 4), indicating that the levels of miR-122 were related to the severity of NAFLD. The levels of miR-29a and miR-122 might be beneficial and compelling as possible biomarkers for non-invasive diagnosis of NAFLD. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

78 FR 53272 - Approval and Promulgation of Air Quality Implementation Plans; Michigan; Redesignation of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-29

... ENVIRONMENTAL PROTECTION AGENCY 40 CFR Parts 52 and 81 [EPA-R05-OAR-2011-0673; FRL-9900-49-Region5... Arbor Area to Attainment of the 1997 Annual Standard and the 2006 24-Hour Standard for Fine Particulate... nonattainment area (Livingston, Macomb, Monroe, Oakland, St. Clair, Washtenaw, and Wayne Counties) to attainment...