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Sample records for ob opredelenii yaderno-fizicheskikh

  1. Walraven photometry of nearby southern OB associations

    NASA Technical Reports Server (NTRS)

    De Geus, E. J.; Lub, J.; Van De Grift, E.

    1990-01-01

    Homogeneous Walraven (VBLUW) photometry is presented for 5260 stars in the regions of five nearby southern OB associations: Scorpio Centaurus (Sco OB2), Orion OB1, Canis Major OB1, Monoceros OB1, and Scutum OB2. Derived V and (B - V) in the Johnson system are included.

  2. Effects of acute exercise over heart proteome from monogenic obese (ob/ob) mice.

    PubMed

    Petriz, Bernardo A; Cunha, Verusca N; Villeth, Gabriela R C; Mehta, Ângela; Rocha, Luiz A O; Silva, Osmar N; Almeida, Jeeser A; Morais, Pamella K; Simões, Herbert G; Franco, Octávio L

    2013-04-01

    Exercise is recognized to prevent and attenuate several metabolic and cardiovascular disorders. Obesity is commonly related to cardiovascular diseases, frequently resulting in heart failure and death. To elucidate the effects of acute exercise in heart tissue from obese animals, 12-week-old C57BL6/J obese (ob/ob) and non-obese (ob/OB) mice were submitted to a single bout of swimming and had their hearts analyzed by proteomic techniques. Mice were divided into three groups: control (ob/ob, n = 3; ob/OB, n = 3); a moderate intensity consisting of 20 min of swimming around 90% of Maximal Lactate Steady State (ob/ob, n = 3; ob/OB, n = 3), and a high intensity exercise performed as an incremental overload test (ob/ob, n = 3; ob/OB, n = 3). Obesity modulations were analyzed by comparing ob/ob and ob/OB control groups. Differential 2-DE analysis revealed that single session of exercise was able to up-regulate: myoglobin (ob/ob), aspartate aminotransferase (ob/OB) and zinc finger protein (ob/OB) and down-regulate: nucleoside diphosphate kinase B (ob/OB), mitochondrial aconitase (ob/ob and ob/OB) and fatty acid binding protein (ob/ob). Zinc finger protein and α-actin were up-regulated by the effect of obesity on heart proteome. These data demonstrate the immediate response of metabolic and stress-related proteins after exercise so as contractile protein by obesity modulation on heart proteome.

  3. Are Field OB Stars Alone?

    NASA Astrophysics Data System (ADS)

    Oey, Sally

    2005-07-01

    This SNAP program offers an inexpensive, simple program to search for low-mass companions of field OB stars. Do field OB stars exist in true isolation, as suggested by a recent Galactic study, or are they the tip of the iceberg on a small cluster of low-mass stars as predicted by the cluster mass function and stellar IMF? Short ACS/WFC V and I observations proposed here may easily resolve this issue for field OB stars in the Small Magellanic Cloud. Truly isolated OB stars represent a theoretical challenge and variation from clusters, in mode of star formation, and have important consequences for our understanding of the field stellar population in galaxies. Small clusters around the field OB stars, on the other hand, may confirm the universality of the stellar clustering law and IMF.

  4. Longitudinal evaluation of hepatic lipid deposition and composition in ob/ob and ob/+ control mice.

    PubMed

    Ye, Qiong; Danzer, Carsten Friedrich; Fuchs, Alexander; Vats, Divya; Wolfrum, Christian; Rudin, Markus

    2013-09-01

    Obesity is associated with insulin resistance (IR) and hepatosteatosis. Understanding the link between IR and hepatosteatosis could be relevant to chronic clinical outcomes. The objective of this study was to quantitatively assess lipid deposition (fractional lipid mass, fLM) and composition (fraction of polyunsaturated lipids, fPUL and mean chain length, MCL) in livers of ob/ob mice, a genetic model of obesity and mild diabetes, and ob/+ heterozygous control animals in a noninvasive manner using (1) H-MRS at 9.4T. For accurate quantification, intensity values were corrected for differences in T2 values while T1 effects were considered minimal due to the long TR values used. Values of fLM, fPUL and MCL were derived from T2 -corrected signal intensities of lipids and water resonance. Hepatic lipid signals were compared with fasted plasma insulin, glucose and lipid levels. Statistically significant correlations between fPUL and fasting plasma insulin/glucose levels were found in adolescent ob/ob mice. A similar correlation was found between fLM and fasting plasma insulin levels; however, the correlation between fLM and fasting plasma glucose levels was less obvious in adolescent ob/ob mice. These correlations were lost in adult ob/ob mice. The study showed that in adolescent ob/ob mice, there was an obvious link between lipid deposition/composition in the liver and plasma insulin/glucose levels. This correlation was lost in adult animals, probably due to the limited lipid storage capacity of the liver.

  5. Peripheral nervous system insulin resistance in ob/ob mice

    PubMed Central

    2013-01-01

    Background A reduction in peripheral nervous system (PNS) insulin signaling is a proposed mechanism that may contribute to sensory neuron dysfunction and diabetic neuropathy. Neuronal insulin resistance is associated with several neurological disorders and recent evidence has indicated that dorsal root ganglion (DRG) neurons in primary culture display altered insulin signaling, yet in vivo results are lacking. Here, experiments were performed to test the hypothesis that the PNS of insulin-resistant mice displays altered insulin signal transduction in vivo. For these studies, nondiabetic control and type 2 diabetic ob/ob mice were challenged with an intrathecal injection of insulin or insulin-like growth factor 1 (IGF-1) and downstream signaling was evaluated in the DRG and sciatic nerve using Western blot analysis. Results The results indicate that insulin signaling abnormalities documented in other “insulin sensitive” tissues (i.e. muscle, fat, liver) of ob/ob mice are also present in the PNS. A robust increase in Akt activation was observed with insulin and IGF-1 stimulation in nondiabetic mice in both the sciatic nerve and DRG; however this response was blunted in both tissues from ob/ob mice. The results also suggest that upregulated JNK activation and reduced insulin receptor expression could be contributory mechanisms of PNS insulin resistance within sensory neurons. Conclusions These findings contribute to the growing body of evidence that alterations in insulin signaling occur in the PNS and may be a key factor in the pathogenesis of diabetic neuropathy. PMID:24252636

  6. Ice Jams the Ob River

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Russia's Ob River flows from south to north, and each summer, it thaws in the same direction. The result is that an ice jam sits downstream from thawed portions of the river, which is laden with heavy runoff from melted snow. On June 29, 2007, the Moderate Resolution Imaging Spectroradiometer (MODIS) flying on NASA's Terra satellite captured this image of the almost completely thawed Ob River. The scene is typical for early summer. South of the ice jam, the Gulf of Ob is swollen with pent-up run-off, and upstream from that, the river is widened as well. Unable to carve through frozen land, the river has little choice but to overflow its banks. For a comparison of early summer and autumn conditions, see Flooding on the Ob River in the Earth Observatory's Natural Hazards section. Besides the annual overflow, this image captures other circumstances of early summer. Sea ice is retreating from the Kara Sea. A lingering line of snow cover snakes its way along the Ob River, to the west. And while the land is lush and green in the south, it appears barren and brown in the north. Near the mouth of the river and the Kara Sea, the land is cold-adapted tundra, with diminutive plants and a short growing season. Just as the ice plugging the river had yet to thaw in the Far North's short summer, the tundra had not yet to greened up either. In this image it still appears lifeless beige. NASA image courtesy Jeff Schmaltz, MODIS Rapid Response Team, Goddard Space Flight Center

  7. Ice Jams the Ob River

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Russia's Ob River flows from south to north, and each summer, it thaws in the same direction. The result is that an ice jam sits downstream from thawed portions of the river, which is laden with heavy runoff from melted snow. On June 29, 2007, the Moderate Resolution Imaging Spectroradiometer (MODIS) flying on NASA's Terra satellite captured this image of the almost completely thawed Ob River. The scene is typical for early summer. South of the ice jam, the Gulf of Ob is swollen with pent-up run-off, and upstream from that, the river is widened as well. Unable to carve through frozen land, the river has little choice but to overflow its banks. For a comparison of early summer and autumn conditions, see Flooding on the Ob River in the Earth Observatory's Natural Hazards section. Besides the annual overflow, this image captures other circumstances of early summer. Sea ice is retreating from the Kara Sea. A lingering line of snow cover snakes its way along the Ob River, to the west. And while the land is lush and green in the south, it appears barren and brown in the north. Near the mouth of the river and the Kara Sea, the land is cold-adapted tundra, with diminutive plants and a short growing season. Just as the ice plugging the river had yet to thaw in the Far North's short summer, the tundra had not yet to greened up either. In this image it still appears lifeless beige. NASA image courtesy Jeff Schmaltz, MODIS Rapid Response Team, Goddard Space Flight Center

  8. Adrenalectomy fails to stimulate brown adipose tissue metabolism in ob/ob mice fed glucose.

    PubMed

    Kim, H K; Romsos, D R

    1988-11-01

    Adrenalectomy arrests the development of obesity in ob/ob mice fed nonpurified high-starch diets partly by stimulating the low thermogenic activity of brown adipose tissue (BAT). However, adrenalectomy fails to suppress the development of obesity in ob/ob mice fed a purified high-glucose diet. Effects of adrenalectomy on BAT metabolism in ob/ob mice fed purified high-starch or high-glucose diets were therefore examined. Adrenalectomy markedly decreased the efficiency of energy retention and increased BAT metabolism (as assessed by GDP binding to BAT mitochondria, GDP-inhibitable acetate- or chloride-induced mitochondrial swelling, and by rates of norepinephrine turnover in BAT) in ob/ob mice fed a high-starch purified diet but had only minimal effects on energy efficiency or BAT metabolism in ob/ob mice fed a high-glucose purified diet. Plasma insulin concentrations decreased and thyroxine concentrations increased in adrenalectomized ob/ob mice fed the high-starch diet; changes in these hormones were less pronounced in adrenalectomized ob/ob mice fed the high-glucose diet. Consumption of glucose mimics effects of adrenal secretions on BAT metabolism in ob/ob mice.

  9. Effect of leptin administration on myelination in ob/ob mouse cerebrum after birth.

    PubMed

    Hashimoto, Ryuju; Matsumoto, Akihiro; Udagawa, Jun; Hioki, Kyoji; Otani, Hiroki

    2013-01-09

    Brain weight and size are known to be reduced in adult leptin-deficient Lep/Lep (OB) mice when compared with the wild-type (+/+) mice (C57BL/6: B6). We here analyzed leptin's effects on myelination by examining morphometrically the myelin sheath (MS) in the cerebrum of postnatal day (P) 14 and P28 OB that had received leptin 1 nmol/capita/day from P7 to P14 or P28 (OB+lep), in comparison with OB and B6. We examined myelin basic protein (MBP) mRNA levels and the differentiation of oligodendrocytes by comparing the number of oligodendrocyte precursor cells (OPCs) and the mature oligodendrocytes in the cerebrum between OB, OB+lep, and B6 on P14 and P28. MBP-mRNA expression was lower in OB than in B6 on P14 and P28. On P14, it was higher in OB+lep than in OB but was still lower than in B6, whereas on P28 it was even higher in OB+lep than in B6. On P28, the radii of myelinated axons were larger in OB than in B6 and OB+lep. The MS on P28 was significantly thinner in OB than in B6, but there was no significant difference between OB and OB+lep. There were significantly fewer mature oligodendrocytes in OB and OB+lep than in B6 on P28, whereas on P14 there were significantly fewer OPCs in OB and OB+lep than in B6. Our results suggested that leptin regulates the myelination of oligodendrocytes and that the replenishment of leptin in OB recovered myelination but did not affect the differentiation of OPCs from P7 to P28.

  10. Effect of leptin treatment on mitochondrial function in obese leptin-deficient ob/ob mice.

    PubMed

    Holmström, Maria H; Tom, Robby Zachariah; Björnholm, Marie; Garcia-Roves, Pablo M; Zierath, Juleen R

    2013-09-01

    Leptin stimulates peripheral lipid oxidation, but the influence on mitochondrial function is partly unknown. We investigated tissue-specific mitochondrial function in leptin-deficient obese C57BL/6J-ob/ob mice compared to lean littermates following leptin treatment. Lean and obese ob/ob mice were treated with saline or leptin for 5 days. At day six, liver, extensor digitorum longus (EDL) and soleus muscle were dissected and mitochondrial respiration analyzed in freshly dissected tissues. Expression of key proteins in the regulation of mitochondrial function was determined. In liver, mitochondrial respiration was reduced in ob/ob mice compared to lean mice. Expression of mitochondrial transcription factor A (TFAM) was decreased in ob/ob mice, but increased with leptin treatment. In glycolytic EDL muscle, mitochondrial respiration was increased in ob/ob mice. Protein markers of complex II, IV and ATP synthase were increased in EDL muscle from both saline- and leptin-treated ob/ob mice. TFAM protein abundance was decreased, while dynamin-1-like protein was increased in EDL muscle from saline-treated ob/ob mice and restored by leptin treatment. In oxidative soleus muscle, mitochondrial respiration and electron transport system protein abundance were unchanged, while TFAM was reduced in ob/ob mice. In conclusion, leptin-deficient ob/ob mice display tissue-specific mitochondrial adaptations under basal conditions and in response to leptin treatment. Mitochondrial respiration was decreased in liver, increased in glycolytic muscle and unaltered in oxidative muscle from ob/ob mice. Insight into the tissue-specific regulation of mitochondrial function in response to energy supply and demand may provide new opportunities for the treatment of insulin resistance. Copyright © 2013 Elsevier Inc. All rights reserved.

  11. Sequentially triggered star formation in OB associations

    NASA Astrophysics Data System (ADS)

    Preibisch, Thomas; Zinnecker, Hans

    We discuss observational evidence for sequential and triggered star formation in galactic and extragalactic OB associations. We will first review in detail the star formation process in the Scorpius-Centaurus OB association, the nearest OB association to the Sun, where several recent extensive studies have provided comprehensive information on the stellar content and the ages of the different OB subgroups. These data have allowed us to reconstruct the star formation history of the association in some detail and provided important insight into the activity in the rho Oph and Lupus dark clouds, and with the origin of several young stellar groups in the southern sky. After discussing evidence for triggered star formation in and around various other Galactic OB associations (e.g. Ori OB1, Per OB2) we will compare the observational results with recent models of rapid star formation in the turbulent interstellar medium (cf. Briceno et al. chapter in Protostars and Planets V, in press).

  12. Role of estrogen receptor signaling in skeletal response to leptin in female ob/ob mice.

    PubMed

    Turner, Russell T; Philbrick, Kenneth A; Kuah, Amida F; Branscum, Adam J; Iwaniec, Urszula T

    2017-06-01

    Leptin, critical in regulation of energy metabolism, is also important for normal bone growth, maturation and turnover. Compared to wild type (WT) mice, bone mass is lower in leptin-deficient ob/ob mice. Osteopenia in growing ob/ob mice is due to decreased bone accrual, and is associated with reduced longitudinal bone growth, impaired cancellous bone maturation and increased marrow adipose tissue (MAT). However, leptin deficiency also results in gonadal dysfunction, disrupting production of gonadal hormones which regulate bone growth and turnover. The present study evaluated the role of increased estrogen in mediating the effects of leptin on bone in ob/ob mice. Three-month-old female ob/ob mice were randomized into one of the 3 groups: (1) ob/ob + vehicle (veh), (2) ob/ob + leptin (leptin) or (3) ob/ob + leptin and the potent estrogen receptor antagonist ICI 182,780 (leptin + ICI). Age-matched WT mice received vehicle. Leptin (40 µg/mouse, daily) and ICI (10 µg/mouse, 2×/week) were administered by subcutaneous injection for 1 month and bone analyzed by X-ray absorptiometry, microcomputed tomography and static and dynamic histomorphometry. Uterine weight did not differ between ob/ob mice and ob/ob mice receiving leptin + ICI, indicating that ICI successfully blocked the uterine response to leptin-induced increases in estrogen levels. Compared to leptin-treated ob/ob mice, ob/ob mice receiving leptin + ICI had lower uterine weight; did not differ in weight loss, MAT or bone formation rate; and had higher longitudinal bone growth rate and cancellous bone volume fraction. We conclude that increased estrogen signaling following leptin treatment is dispensable for the positive actions of leptin on bone and may attenuate leptin-induced bone growth. © 2017 Society for Endocrinology.

  13. OB or Not OB: Idiosyncratic utilization of the tRNA-binding OB-fold domain in unicellular, pathogenic eukaryotes.

    PubMed

    Kapps, Delphine; Cela, Marta; Théobald-Dietrich, Anne; Hendrickson, Tamara; Frugier, Magali

    2016-12-01

    In this review, we examine the so-called OB-fold, a tRNA-binding domain homologous to the bacterial tRNA-binding protein Trbp111. We highlight the ability of OB-fold homologs to bind tRNA species and summarize their distribution in evolution. Nature has capitalized on the advantageous effects acquired when an OB-fold domain binds to tRNA by evolutionarily selecting this domain for fusion to different enzymes. Here, we review our current understanding of how the complexity of OB-fold-containing proteins and enzymes developed to expand their functions, especially in unicellular, pathogenic eukaryotes.

  14. Gender-specific differences in diabetic neuropathy in BTBR ob/ob mice

    PubMed Central

    O’Brien, Phillipe D.; Hur, Junguk; Robell, Nicholas J.; Hayes, John M.; Sakowski, Stacey A.; Feldman, Eva L.

    2015-01-01

    Aims To identify a female mouse model of diabetic peripheral neuropathy (DPN), we characterized DPN in female BTBR ob/ob mice and compared their phenotype to non-diabetic and gender-matched controls. We also identified dysregulated genes and pathways in sciatic nerve (SCN) and dorsal root ganglia (DRG) of female BTBR ob/ob mice to determine potential DPN mechanisms. Methods Terminal neuropathy phenotyping consisted of examining latency to heat stimuli, sciatic motor and sural sensory nerve conduction velocities (NCV), and intraepidermal nerve fiber (IENF) density. For gene expression profiling, DRG and SCN were dissected, RNA was isolated and processed using microarray technology and differentially expressed genes were identified. Results Similar motor and sensory NCV deficits were observed in male and female BTBR ob/ob mice at study termination; however, IENF density was greater in female ob/ob mice than their male counterparts. Male and female ob/ob mice exhibited similar weight gain, hyperglycemia, and hyperinsulinemia compared to non-diabetic controls, although triglycerides were elevated more so in males than in females. Transcriptional profiling of nerve tissue from female mice identified dysregulation of pathways related to inflammation. Conclusions Similar to males, female BTBR ob/ob mice display robust DPN, and pathways related to inflammation are dysregulated in peripheral nerve. PMID:26525588

  15. The Nearest OB Association: Scorpius-Centaurus (Sco OB2)

    NASA Astrophysics Data System (ADS)

    Preibisch, T.; Mamajek, E.

    2008-12-01

    We summarize observational results on the stellar population and star formation history of the Scorpius-Centaurus OB Association (Sco OB2), the nearest region of recent massive star formation. It consists of three subgroups, Upper Scorpius (US), Upper Centaurus-Lupus (UCL), and Lower Centaurus-Crux (LCC) which have ages of about 5, 17, and 16 Myr. While the high- and intermediate mass association members have been studied for several decades, the low-mass population remained mainly unexplored until rather recently. In Upper Scorpius, numerous studies, in particular large multi-object spectroscopic surveys, have recently revealed hundreds of low-mass association members, including dozens of brown dwarfs. The investigation of a large representative sample of association members provided detailed information about the stellar population and the star formation history. The empirical mass function could be established over the full stellar mass range from 0.1 M_⊙ up to 20 M_⊙, and was found to be consistent with recent determinations of the field initial mass function. A narrow range of ages around 5 Myr was found for the low-mass stars, the same age as had previously (and independently) been derived for the high-mass members. This supports earlier indications that the star formation process in US was triggered, and agrees with previous conjectures that the triggering event was a supernova- and wind-driven shock-wave originating from the nearby UCL group. In the older UCL and LCC regions, large numbers of low-mass members have recently been identified among X-ray and proper-motion selected candidates. In both subgroups, low-mass members have also been serendipitously discovered through investigations of X-ray sources in the vicinity of better known regions (primarily the Lupus and TW Hya associations). While both subgroups appear to have mean ages of ˜16 Myr, they both show signs of having substructure. Their star-formation histories may be more complex than that

  16. Chemical evolution of OB associations

    NASA Technical Reports Server (NTRS)

    Schramm, D. N.; Olive, K. A.

    1982-01-01

    It is determined that the existence of Al-26 and Pd-107 in meteorites in the early solar system indicates that our solar system probably formed inside an OB association that had been contaminated by the debris of at least one supernova. In addition to these radioactive tracers, the contamination of the material out of which the solar system formed would have significantly enriched the heavy element composition of the solar system relative to that of the average interstellar medium. It is found that the solar system would be enriched in those isotopes which are produced by the more massive stars, such as O-16, C-12, Ne-20, and some other r-process material. It is proposed that specific isotopic ratios and elemental ratios reflecting these differences would include the Ne-20/Ne-22 ratio, which would be higher in the solar system than in the interstellar medium and the cosmic rays, the C-12/C-13 ratio which would be higher in the solar system than in the interstellar medium, and the oxygen-to-carbon ratio, which would also be higher in the solar system than in the typical interstellar medium.

  17. Hydrogen sulfide improves diabetic wound healing in ob/ob mice via attenuating inflammation.

    PubMed

    Zhao, Huichen; Lu, Shengxia; Chai, Jiachao; Zhang, Yuchao; Ma, Xiaoli; Chen, Jicui; Guan, Qingbo; Wan, Meiyan; Liu, Yuantao

    2017-09-01

    The proposed mechanisms of impaired wound healing in diabetes involve sustained inflammation, excess oxidative stress and compromised agiogenesis. Hydrogen sulfide (H2S) has been reported to have multiple biological activities. We aim to investigate the role of H2S in impaired wound healing in ob/ob mice and explore the possible mechanisms involved. Full-thickness skin dorsal wounds were created on ob/ob mice and C57BL/6 mice. Cystathionine-γ-lyase (CSE) expression and H2S production were determined in granulation tissues of the wounds. Effects of NaHS on wound healing were evaluated. Inflammation and angiogenesis in granulation tissues of the wounds were examined. CSE expression, and H2S content were significantly reduced in granulation tissues of wounds in ob/ob mice compared with control mice. NaHS treatment significantly improved wound healing in ob/ob mice, which was associated with reduced neutrophil and macrophage infiltration, decreased production of tumor necrosis factor (TNF)-α, interleukin (IL)-6. NaHS treatment decreased metalloproteinase (MMP)-9, whereas increased collagen deposition and vascular-like structures in granulation tissues of wounds in ob/ob mice. CSE down-regulation may play a role in the pathogenesis of diabetic impaired wound healing. Exogenous H2S could be a potential agent to improve diabetic impaired wound healing by attenuating inflammation and increasing angiogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Impairment of peripheral circadian clocks precedes metabolic abnormalities in ob/ob mice.

    PubMed

    Ando, Hitoshi; Kumazaki, Masafumi; Motosugi, Yuya; Ushijima, Kentarou; Maekawa, Tomohiro; Ishikawa, Eiko; Fujimura, Akio

    2011-04-01

    Recent studies have demonstrated relationships between the dysfunction of circadian clocks and the development of metabolic abnormalities, but the chicken-and-egg question remains unresolved. To address this issue, we investigated the cause-effect relationship in obese, diabetic ob/ob mice. Compared with control C57BL/6J mice, the daily mRNA expression profiles of the clock and clock-controlled genes Clock, Bmal1, Cry1, Per1, Per2, and Dbp were substantially dampened in the liver and adipose tissue, but not the hypothalamic suprachiasmatic nucleus, of 10-wk-old ob/ob mice. Four-week feeding of a low-calorie diet and administration of leptin over a 7-d period attenuated, to a significant and comparable extent, the observed metabolic abnormalities (obesity, hyperglycemia, hyperinsulinemia, and hypercholesterolemia) in the ob/ob mice. However, only leptin treatment improved the impaired peripheral clocks. In addition, clock function, assessed by measuring levels of Per1, Per2, and Dbp mRNA at around peak times, was also reduced in the peripheral tissues of 3-wk-old ob/ob mice without any overt metabolic abnormalities. Collectively these results indicate that the impairment of peripheral clocks in ob/ob mice does not result from metabolic abnormalities but may instead be at least partially caused by leptin deficiency itself. Further studies are needed to clarify how leptin deficiency affects peripheral clocks.

  19. Ob/ob Mouse Livers Show Decreased Oxidative Phosphorylation Efficiencies and Anaerobic Capacities after Cold Ischemia

    PubMed Central

    Tagaloa, Sherry; Zhang, Linda; Dare, Anna J.; MacDonald, Julia R.; Yeong, Mee-Ling; Bartlett, Adam S. J. R.; Phillips, Anthony R. J.

    2014-01-01

    Background Hepatic steatosis is a major risk factor for graft failure in liver transplantation. Hepatic steatosis shows a greater negative influence on graft function following prolonged cold ischaemia. As the impact of steatosis on hepatocyte metabolism during extended cold ischaemia is not well-described, we compared markers of metabolic capacity and mitochondrial function in steatotic and lean livers following clinically relevant durations of cold preservation. Methods Livers from 10-week old leptin-deficient obese (ob/ob, n = 9) and lean C57 mice (n = 9) were preserved in ice-cold University of Wisconsin solution. Liver mitochondrial function was then assessed using high resolution respirometry after 1.5, 3, 5, 8, 12, 16 and 24 hours of storage. Metabolic marker enzymes for anaerobiosis and mitochondrial mass were also measured in conjunction with non-bicarbonate tissue pH buffering capacity. Results Ob/ob and lean mice livers showed severe (>60%) macrovesicular and mild (<30%) microvesicular steatosis on Oil Red O staining, respectively. Ob/ob livers had lower baseline enzymatic complex I activity but similar adenosine triphosphate (ATP) levels compared to lean livers. During cold storage, the respiratory control ratio and complex I-fueled phosphorylation deteriorated approximately twice as fast in ob/ob livers compared to lean livers. Ob/ob livers also demonstrated decreased ATP production capacities at all time-points analyzed compared to lean livers. Ob/ob liver baseline lactate dehydrogenase activities and intrinsic non-bicarbonate buffering capacities were depressed by 60% and 40%, respectively compared to lean livers. Conclusions Steatotic livers have impaired baseline aerobic and anaerobic capacities compared to lean livers, and mitochondrial function indices decrease particularly from after 5 hours of cold preservation. These data provide a mechanistic basis for the clinical recommendation of shorter cold storage durations in steatotic donor

  20. Ob/ob mouse livers show decreased oxidative phosphorylation efficiencies and anaerobic capacities after cold ischemia.

    PubMed

    Chu, Michael J J; Hickey, Anthony J R; Tagaloa, Sherry; Zhang, Linda; Dare, Anna J; MacDonald, Julia R; Yeong, Mee-Ling; Bartlett, Adam S J R; Phillips, Anthony R J

    2014-01-01

    Hepatic steatosis is a major risk factor for graft failure in liver transplantation. Hepatic steatosis shows a greater negative influence on graft function following prolonged cold ischaemia. As the impact of steatosis on hepatocyte metabolism during extended cold ischaemia is not well-described, we compared markers of metabolic capacity and mitochondrial function in steatotic and lean livers following clinically relevant durations of cold preservation. Livers from 10-week old leptin-deficient obese (ob/ob, n = 9) and lean C57 mice (n = 9) were preserved in ice-cold University of Wisconsin solution. Liver mitochondrial function was then assessed using high resolution respirometry after 1.5, 3, 5, 8, 12, 16 and 24 hours of storage. Metabolic marker enzymes for anaerobiosis and mitochondrial mass were also measured in conjunction with non-bicarbonate tissue pH buffering capacity. Ob/ob and lean mice livers showed severe (>60%) macrovesicular and mild (<30%) microvesicular steatosis on Oil Red O staining, respectively. Ob/ob livers had lower baseline enzymatic complex I activity but similar adenosine triphosphate (ATP) levels compared to lean livers. During cold storage, the respiratory control ratio and complex I-fueled phosphorylation deteriorated approximately twice as fast in ob/ob livers compared to lean livers. Ob/ob livers also demonstrated decreased ATP production capacities at all time-points analyzed compared to lean livers. Ob/ob liver baseline lactate dehydrogenase activities and intrinsic non-bicarbonate buffering capacities were depressed by 60% and 40%, respectively compared to lean livers. Steatotic livers have impaired baseline aerobic and anaerobic capacities compared to lean livers, and mitochondrial function indices decrease particularly from after 5 hours of cold preservation. These data provide a mechanistic basis for the clinical recommendation of shorter cold storage durations in steatotic donor livers.

  1. Ablation of ghrelin receptor in leptin-deficient ob/ob mice has paradoxical effects on glucose homeostasis when compared with ablation of ghrelin in ob/ob mice

    USDA-ARS?s Scientific Manuscript database

    The orexigenic hormone ghrelin is important in diabetes because it has an inhibitory effect on insulin secretion. Ghrelin ablation in leptin-deficient ob/ob (Ghrelin(-/-):ob/ob) mice increases insulin secretion and improves hyperglycemia. The physiologically relevant ghrelin receptor is the growth ...

  2. Leptin differentially regulates STAT3 activation in the ob/ob mice adipose mesenchymal stem cells

    USDA-ARS?s Scientific Manuscript database

    Leptin-deficient genetically obese ob/ob mice exhibit adipocyte hypertrophy and hyperplasia as well as elevated adipose tissue and systemic inflammation. Studies have shown that multipotent stem cells isolated from adult adipose tissue can differentiate into adipocytes ex vivo and thereby contribute...

  3. Efficient Method of Genotyping Ob/Ob Mice Using High Resolution Melting Analysis

    PubMed Central

    Kurtz, Nichole; Spyropoulos, Demetri D.; Chavin, Kenneth D.

    2013-01-01

    Objective Direct health care costs of obesity continue to grow throughout the world and research on obesity disease models are on the rise. The ob/ob mouse is a well-characterized model of obesity and associated risk factors. Successful breeding and backcrossing onto different backgrounds are essential to create knockout models. Ob/ob mice are sterile and heterozygotes must be identified by genotyping to maintain breeding colonies. Several methods are employed to detect the ob mutant allele, a single nucleotide polymorphism (SNP). Gel based methods are time consuming and inconsistent, and non-gel based assays rely upon expensive and complex reagents or instruments. A fast, high-throughput, cost effective, and consistent method to identify Lepob mutation is much needed. Design and Methods Primers to produce an amplicon for High Resolution Melting Analysis (HRM) of the Lepob SNP were designed and validated. Results Fluorescence normalized high resolution melting curve plots delineated ob/+, ob/ob, and WT genotypes. Genotypes were also confirmed phenotypically. Conclusions HRM of the Lepob SNP allows closed-tube identification of the Lepob mutation using a real-time PCR machine now common to most labs/departments. Advantages of this method include assay sensitivity/accuracy, low cost dyes, less optimization, and cost effectiveness as compared to other genotyping techniques. PMID:24236058

  4. Complete Genome Sequence of Escherichia coli Strain M8, Isolated from ob/ob Mice

    PubMed Central

    Siddharth, Jay; Membrez, Mathieu; Chakrabarti, Anirikh; Betrisey, Bertrand; Chou, Chieh Jason

    2017-01-01

    ABSTRACT Escherichia coli is one of the common inhabitants of the mammalian gastrointestinal track. We isolated a strain from an ob/ob mouse and performed whole-genome sequencing, which yielded a chromosome of ~5.1 Mb and three plasmids of ~160 kb, ~6 kb, and ~4 kb. PMID:28572322

  5. Gut microbiota-associated bile acid deconjugation accelerates hepatic steatosis in ob/ob mice.

    PubMed

    Park, M-Y; Kim, S J; Ko, E K; Ahn, S-H; Seo, H; Sung, M-K

    2016-09-01

    Nonalcoholic hepatic fat accumulation has been hypothesized to be associated with alterations in gut microbiota composition, although mechanistic explanations for this link are largely insufficient. The aim of this study was to elucidate the microbiota-driven mechanisms involved in the development of nonalcoholic hepatic steatosis. Ob/ob mice and their wild-type lean control mice were fed an AIN-93G diet for 12 weeks. Faecal microbiota composition, faecal bile acid (BA) profile and intestinal and hepatic markers of BA metabolism were analysed. Ob/ob mice had significantly less faecal taurine-conjugated BAs compared to their lean controls. The proportions of butyrate-producing bacteria were lower in ob/ob mice compared to those in lean mice. Intestinal expression of farnesoid X receptor (FXR) mRNA was significantly higher, whereas hepatic expression of cholesterol-7α-hydroxylase 1 (CYP7A1) and small heterodimer partner (SHP) were significantly lower in ob/ob mice compared to those in control mice. Microbiota-associated BAs deconjugation may induce nonalcoholic fatty liver disease (NAFLD) by activating intestinal FXR signalling and blocking hepatic FXR-SHP pathway, thereby accelerating fat synthesis. We provided evidences that changes in the gut microbiota and their metabolites can alter the profile of BAs, thereby providing a mechanism by which an altered microbiota profile contributes to the development of NAFLD. © 2016 The Society for Applied Microbiology.

  6. The MicrOBS study around Taiwan

    NASA Astrophysics Data System (ADS)

    Liang, C.; Lee, C.

    2007-12-01

    Since 2005, we have managed 16 MicrOBS, designed by the French IFREMER, to study the earthquake and crustal structute around Taiwan. The instrument itself is very light (20 kgs) and easy to operate. This OBS has a measuring period up to 2 weeks in the seafloor. We have conducted the works, aiming to get a better understanding of the ¡§marine¡¨ earthquakes, from the in situ small event to the big earthquake. For example, a study of the seismic gaps in the offshore region becomes workable as compare with the time when we only have the land seismological instruments. We have also used this instrument to obtain a detail study of the gas hydrate and free gas zone. Whenever a large seismic source is available, we then conduct the local and regional crustal studies, such as in the Philippine Sea and South China Sea. In the last 3 years, we have directed 285 deployments of MicrOBS around Taiwan. More than 80 per cent of the natural earthquakes in Taiwan (in an average of about 15,000 events of Mm greater than 2 events per year) were occurred in offshore area. Big earthquakes (i.e. Mm greater than 6) are more often happen in the offshore area than that in the onshore region. Therefore, the tools (MicrOBS and OBS) to measure these ¡§marine¡¨ earthquakes become essential to understand the character and origin of crustal dynamics, particularly in the seismogenic zone.

  7. Sustained activation of PPARα by endogenous ligands increases hepatic fatty acid oxidation and prevents obesity in ob/ob mice.

    PubMed

    Huang, Jiansheng; Jia, Yuzhi; Fu, Tao; Viswakarma, Navin; Bai, Liang; Rao, M Sambasiva; Zhu, Yijun; Borensztajn, Jayme; Reddy, Janardan K

    2012-02-01

    Obesity, a major health concern, results from an imbalance between energy intake and expenditure. Leptin-deficient ob/ob mice are paradigmatic of obesity, resulting from excess energy intake and storage. Mice lacking acyl-CoA oxidase 1 (Acox1), the first enzyme of the peroxisomal fatty acid β-oxidation system, are characterized by increased energy expenditure and a lean body phenotype caused by sustained activation of peroxisome proliferator-activated receptor α (PPARα) by endogenous ligands in liver that remain unmetabolized in the absence of Acox1. We generated ob/ob mice deficient in Acox1 (Acox1(-/-)) to determine how the activation of PPARα by endogenous ligands might affect the obesity of ob/ob mice. In contrast to Acox1(-/-) (14.3±1.2 g at 6 mo) and the Acox1-deficient (ob/ob) double-mutant mice (23.8±4.6 g at 6 mo), the ob/ob mice are severely obese (54.3±3.2 g at 6 mo) and had significantly more (P<0.01) epididymal fat content. The resistance of Acox1(-/-)/ob/ob mice to obesity is due to increased PPARα-mediated up-regulation of genes involved in fatty acid oxidation in liver. Activation of PPARα in Acox1-deficient ob/ob mice also reduces serum glucose and insulin (P<0.05) and improves glucose tolerance and insulin sensitivity. Further, PPARα activation reduces hepatic steatosis and increases hepatocellular regenerative response in Acox1(-/-)/ob/ob mice at a more accelerated pace than in mice lacking only Acox1. However, Acox1(-/-)/ob/ob mice manifest hepatic endoplasmic reticulum (ER) stress and also develop hepatocellular carcinomas (8 of 8 mice) similar to those observed in Acox1(-/-) mice (10 of 10 mice), but unlike in ob/ob (0 of 14 mice) and OB/OB (0 of 6 mice) mice, suggesting that superimposed ER stress and PPARα activation contribute to carcinogenesis in a fatty liver. Finally, absence of Acox1 in ob/ob mice can impart resistance to high-fat diet (60% fat)-induced obesity, and their liver had significantly (P<0.01) more cell

  8. Effects of adrenalectomy on energy balance in obese (ob/ob) mice fed high carbohydrate or high fat diets.

    PubMed

    Grogan, C K; Kim, H K; Romsos, D R

    1987-06-01

    We reported previously that adrenalectomy reduced the energy density of body weight gain (an indicator of proportional gain in lean and fat tissue) and the efficiency of energy retention in obese (ob/ob) mice to values approximating those in lean mice, but that adrenalectomy had much less influence on these parameters in ob/ob mice fed a purified high fat diet. To determine if fat was the exclusive factor in the purified high fat diet that negated effects of adrenalectomy, ob/ob mice were fed a purified high carbohydrate (glucose) diet identical in composition to the high fat diet, except for the fat/carbohydrate ratio. Responses of adrenalectomized ob/ob mice fed the purified high glucose diet from 4 to 7 wk of age mimicked those of mice fed the purified high fat diet, not those of mice fed the high carbohydrate nonpurified diet. Plasma glucose responses to a glucose load in adrenalectomized ob/ob mice paralleled the diet-dependent changes in energy balance. These results demonstrate that diet composition interacts with adrenal secretions to influence energy and glucose metabolism in ob/ob mice; consumption of either a purified high glucose or high fat diet negates the beneficial effects of adrenalectomy on energy and glucose metabolism observed when adrenalectomized ob/ob mice consume a nonpurified diet.

  9. Captopril Normalizes Insulin Signaling and Insulin-Regulated Substrate Metabolism in Obese (ob/ob) Mouse Hearts

    PubMed Central

    Tabbi-Anneni, Imene; Buchanan, Jonathan; Cooksey, Robert C.; Abel, E. Dale

    2008-01-01

    The goal of this study was to determine whether inhibiting the renin-angiotensin system would restore insulin signaling and normalize substrate use in hearts from obese ob/ob mice. Mice were treated for 4 wk with Captopril (4 mg/kg·d). Circulating levels of free fatty acids, triglycerides, and insulin were measured and glucose tolerance tests performed. Rates of palmitate oxidation and glycolysis, oxygen consumption, and cardiac power were determined in isolated working hearts in the presence and absence of insulin, along with levels of phosphorylation of Akt and AMP-activated protein kinase (AMPK). Captopril treatment did not correct the hyperinsulinemia or impaired glucose tolerance in ob/ob mice. Rates of fatty acid oxidation were increased and glycolysis decreased in ob/ob hearts, and insulin did not modulate substrate use in hearts of ob/ob mice and did not increase Akt phosphorylation. Captopril restored the ability of insulin to regulate fatty acid oxidation and glycolysis in hearts of ob/ob mice, possibly by increasing Akt phosphorylation. Moreover, AMPK phosphorylation, which was increased in hearts of ob/ob mice, was normalized by Captopril treatment, suggesting that in addition to restoring insulin sensitivity, Captopril treatment improved myocardial energetics. Thus, angiotensin-converting enzyme inhibitors restore the responsiveness of ob/ob mouse hearts to insulin and normalizes AMPK activity independently of effects on systemic metabolic homeostasis. PMID:18450963

  10. Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice.

    PubMed

    Bassi, M; Furuya, W I; Menani, J V; Colombari, D S A; do Carmo, J M; da Silva, A A; Hall, J E; Moreira, T S; Wenker, I C; Mulkey, D K; Colombari, E

    2014-05-01

    Leptin, an adipocyte-derived hormone, is suggested to participate in the central control of breathing. We hypothesized that leptin may facilitate ventilatory responses to chemoreflex activation by acting on respiratory nuclei of the ventrolateral medulla. The baseline ventilation and the ventilatory responses to CO2 were evaluated before and after daily injections of leptin into the retrotrapezoid nucleus/parafacial respiratory group (RTN/pFRG) for 3 days in obese leptin-deficient (ob/ob) mice. Male ob/ob mice (40-45 g, n = 7 per group) received daily microinjections of vehicle or leptin (1 μg per 100 nL) for 3 days into the RTN/pFRG. Respiratory responses to CO2 were measured by whole-body plethysmography. Unilateral microinjection of leptin into the RTN/pFRG in ob/ob mice increased baseline ventilation (VE ) from 1447 ± 96 to 2405 ± 174 mL min(-1) kg(-1) by increasing tidal volume (VT ) from 6.4 ± 0.4 to 9.1 ± 0.8 mL kg(-1) (P < 0.05). Leptin also enhanced ventilatory responses to 7% CO2 (Δ = 2172 ± 218 mL min(-1) kg(-1) , vs. control: Δ = 1255 ± 105 mL min(-1) kg(-1) ), which was also due to increased VT (Δ = 4.71 ± 0.51 mL kg(-1) , vs. control: Δ = 2.27 ± 0.20 mL kg(-1) ), without changes in respiratory frequency. Leptin treatment into the RTN/pFRG or into the surrounding areas decreased food intake (83 and 70%, respectively), without significantly changing body weight. The present results suggest that leptin acting in the respiratory nuclei of the ventrolateral medulla improves baseline VE and VT and facilitates respiratory responses to hypercapnia in ob/ob mice. © 2014 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

  11. Effects of resveratrol on ovarian response to controlled ovarian hyperstimulation in ob/ob mice.

    PubMed

    Cabello, Estefanía; Garrido, Pablo; Morán, Javier; González del Rey, Carmen; Llaneza, Plácido; Llaneza-Suárez, David; Alonso, Ana; González, Celestino

    2015-02-01

    To evaluate antidiabetic and anti-inflammatory effects of resveratrol on the ovarian response to controlled ovarian hyperstimulation (COH) in obesity-related infertility. Experimental. University laboratory. Sixteen female ob/ob mice and 16 female C57BL/6J mice undergoing COH. Wild-type placebo group; wild-type resveratrol group; ob/ob mice placebo group; ob/ob mice resveratrol group. Resveratrol 3.75 mg/kg daily for 20 days and undergoing COH protocol. Body and reproductive system weight, food intake, fasting blood glucose, plasma insulin and T levels, and Homeostatic Index of Insulin Resistance; interleukin-6 and tumor necrosis factor-α levels in adipose tissue by Western blot; assessment of quality and quantity of oocytes retrieved; and quantitative analysis of ovarian follicles. Plasma insulin and T levels decreased and Homeostatic Index of Insulin Resistance improved in ob/ob mice treated with resveratrol. Interleukin-6 and tumor necrosis factor-α levels were significantly reverted back to near normalcy after resveratrol treatment in obese mice. Administration of resveratrol resulted in a significantly higher number of oocytes collected in wild-type mice. The number of primary, growing, preovulatory, and atretic follicles was found to be decreased in the group of obese mice treated with resveratrol when compared with the obese control group. Resveratrol administration could exert benefits against loss of ovarian follicles, and these actions may be mediated, at least in part, via anti-inflammatory, insulin-sensitizing, and antihyperandrogenism effects. These observations further validate the therapeutic potential of resveratrol to preserve ovarian reserve in conditions associated with obesity. Our results suggest the possible clinical use of resveratrol to enhance the ovarian response to COH in normal-weight females. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  12. Selective T-type calcium channel blockade alleviates hyperalgesia in ob/ob mice.

    PubMed

    Latham, Janelle R; Pathirathna, Sriyani; Jagodic, Miljen M; Choe, Won Joo; Levin, Michaela E; Nelson, Michael T; Lee, Woo Yong; Krishnan, Kathiresan; Covey, Douglas F; Todorovic, Slobodan M; Jevtovic-Todorovic, Vesna

    2009-11-01

    Morbid obesity may be accompanied by diabetes and painful diabetic neuropathy, a poorly understood condition that is manifested by mechanical or thermal allodynia and hyperalgesia. Recent studies have highlighted the importance of T-type calcium channels (T-channels) in peripheral nociception; therefore, our goal was to examine the function of these channels in the pathophysiology and development of painful diabetic neuropathy. In vivo testing of mechanical and thermal sensation, morphometric peripheral nerve studies, and electrophysiological and biochemical measurements were used to characterize the role of T-channels and the development of painful diabetic neuropathy in leptin-deficient (ob/ob) mice. We found that ob/ob mice developed significant mechanical and thermal hypersensitivity early in life that coincided with hyperglycemia and was readily reversed with insulin therapy. These disturbances were accompanied by significant biophysical and biochemical modulation of T-channels in dorsal root ganglion neurons as measured by a large increase in the amplitude of T-currents and the expression of mRNA. The most prevalent subtype, alpha1H (Ca(v)3.2), was most strongly affected. Moreover, (3beta,5alpha,17beta)-17-hydroxyestrane-3-carbonitrile (ECN), a novel neuroactive steroid and selective T-channel antagonist, provided dose-dependent alleviation of neuropathic thermal and mechanical hypersensitivity in diabetic ob/ob mice. Our results indicate that pharmacological antagonism of T-channels is potentially an important novel therapeutic approach for the management of painful diabetic neuropathy.

  13. Nobiletin improves hyperglycemia and insulin resistance in obese diabetic ob/ob mice.

    PubMed

    Lee, Young-Sil; Cha, Byung-Yoon; Saito, Kiyoto; Yamakawa, Hiroshi; Choi, Sun-Sil; Yamaguchi, Kohji; Yonezawa, Takayuki; Teruya, Toshiaki; Nagai, Kazuo; Woo, Je-Tae

    2010-06-01

    Nobiletin is a polymethoxylated flavone found in certain citrus fruits that exhibits various pharmacological effects including anti-inflammatory, antitumor and neuroprotective properties. The present study investigated the effects of nobiletin on insulin sensitivity in obese diabetic ob/ob mice, and the possible mechanisms involved. The ob/ob mice were treated with nobiletin (200mg/kg) for 5 weeks. Nobiletin significantly improved the plasma glucose levels, homeostasis model assessment index, glucose tolerance in an oral glucose tolerance test and plasma adiponectin levels. In white adipose tissue (WAT), nobiletin significantly decreased the mRNA expression levels of inflammatory adipokines such as interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 and increased the mRNA expression levels of adiponectin, peroxisome proliferator-activated receptor (PPAR)-gamma and its target genes. At the same time, nobiletin increased the glucose transporter (Glut) 4 expression levels in the whole plasma membrane, and Glut1 and phospho-Akt expression in the whole cell lysates in WAT and muscle. Nobiletin also increased Glut4 protein expression level in the whole cell lysates of the muscle. Taken together, the present results suggest that nobiletin improved the hyperglycemia and insulin resistance in obese diabetic ob/ob mice by regulating expression of Glut1 and Glut4 in WAT and muscle, and expression of adipokines in WAT.

  14. Cordyceps militaris alleviates non-alcoholic fatty liver disease in ob/ob mice

    PubMed Central

    Choi, Ha-Neul; Jang, Yang-Hee; Kim, Min-Joo; Seo, Min Jeong; Kang, Byoung Won; Jeong, Yong Kee

    2014-01-01

    BACKGROUND/OBJECTIVES Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolic syndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris (C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris water extract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), and proinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulin resistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatic total lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS These results indicate that C. militaris can exert protective effects against development of NAFLD, partly by reducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice. PMID:24741401

  15. Longitudinal evaluation of intramyocellular lipids (IMCLs) in tibialis anterior muscle of ob/ob and ob/+ control mice using a cryogenic surface coil at 9.4 T.

    PubMed

    Ye, Qiong; Danzer, Carsten Friedrich; Fuchs, Alexander; Krek, Wilhelm; Mueggler, Thomas; Baltes, Christof; Rudin, Markus

    2011-12-01

    Insulin resistance is a central feature of type II diabetes and is associated with alterations in skeletal muscle lipid metabolism, which manifest themselves, in part, in increased intramyocellular lipid (IMCL) accumulation. The objective of this study was to assess noninvasively the levels of IMCL longitudinally in the tibialis anterior muscle of Lep(ob) /Lep(ob) (ob/ob) mice, a genetic model of obesity and mild diabetes, and Lep(ob) /+ (ob/+) heterozygous control animals, using (1) H MRS at 9.4 T. The use of a cryogenic surface coil transceiver leads to significant increases in sensitivity. Method implementation included the assessment of the reproducibility and spatial heterogeneity of the IMCL signal and the determination of T(2) relaxation times, as IMCL levels were expressed relative to the total creatine signal, and therefore the signal ratios had to be corrected for differences in T(2) relaxation. IMCL levels were found to be significantly higher in ob/ob mice relative to ob/+ heterozygous control mice that do not develop disease. An increase in IMCL levels was observed for ob/ob mice until weeks 16/17; after this time point, IMCL levels decreased again, reaching final levels that were slightly higher than the initial values. These noninvasively detected alterations in skeletal muscle lipid metabolism in ob/ob mice were accompanied by a transient increase in plasma insulin concentrations. This study indicates that IMCL may be reliably assessed in mouse tibialis anterior muscle using a cryogenic surface coil, implying that (1) H MRS at 9.4 T represents a useful technology for the noninvasive measurement of changes in lipid metabolism in the skeletal muscle that accompany obesity.

  16. Switching performance of OBS network model under prefetched real traffic

    NASA Astrophysics Data System (ADS)

    Huang, Zhenhua; Xu, Du; Lei, Wen

    2005-11-01

    Optical Burst Switching (OBS) [1] is now widely considered as an efficient switching technique in building the next generation optical Internet .So it's very important to precisely evaluate the performance of the OBS network model. The performance of the OBS network model is variable in different condition, but the most important thing is that how it works under real traffic load. In the traditional simulation models, uniform traffics are usually generated by simulation software to imitate the data source of the edge node in the OBS network model, and through which the performance of the OBS network is evaluated. Unfortunately, without being simulated by real traffic, the traditional simulation models have several problems and their results are doubtable. To deal with this problem, we present a new simulation model for analysis and performance evaluation of the OBS network, which uses prefetched IP traffic to be data source of the OBS network model. The prefetched IP traffic can be considered as real IP source of the OBS edge node and the OBS network model has the same clock rate with a real OBS system. So it's easy to conclude that this model is closer to the real OBS system than the traditional ones. The simulation results also indicate that this model is more accurate to evaluate the performance of the OBS network system and the results of this model are closer to the actual situation.

  17. Alpha-1 adrenoceptors in brown adipose tissue of lean and ob/ob mice

    SciTech Connect

    Behrens-Zaror, G.; Himms-Hagen, J.

    1986-03-01

    Obese (ob/ob) mice have a low capacity to increase thyroxine 5'-deiodinase (T4 5'-D) in brown adipose tissue (BAT) when exposed to cold. This effect is mediated by alpha-1 (A-1) adrenoceptors. The authors objective was to find out whether BAT of the ob/ob mouse has normal A-1 receptors. Saturation analysis of binding of (3H)-WB4101 at 0.05 nM to 10 ..mu..M to crude membrane preparations (100,000 g pellets from Polytron homogenates) using the LIGAND program of Munson and Rodbard, showed two populations of binding sites in BAT of lean (+/+, 11-15 wk old) mice. Acute exposure (12 h, 14/sup 0/C) or acclimation to cold (3 wk, 14/sup 0/C) did not alter affinity or concentration of sites. Displacement with yohimbine and prazosin indicated binding of WB4101 to A-1 receptors. Very young (5 wk) lean (+/.) and obese mice had similar affinity constants (lean 0.13 +/- 0.043 and 34.2 +/- 14.9; obese, 0.12 +/- 0.028 and 20.9 +/- 5.48 nM) and concentrations (lean 22.4 +/- 3.8 and 647 +/- 137; obese, 28.6 +/- 4.6 and 547 +/- 105 fmol/mg protein) of sites. Old (1 yr) mice had high affinity sites similar to those in younger animals (KD lean 0.19 +/- 0.028, obese, 0.25 +/- 0.075; Bmax lean, 60.2 +/- 12.1; obese, 63.1 +/- 13.5 fmol/mg protein). The authors conclude that the ob/ob mouse has normal high affinity A-1 receptors in BAT. Anomalous properties of low affinity binding in old ob/ob mice could not be characterized because of high nonspecific binding. BAT of the ob/ob mouse does not lack A-1 receptors but may have a post-receptor alteration in the A-1 adrenoceptor-mediated response.

  18. Short-term lenalidomide (Revlimid) administration ameliorates cardiomyocyte contractile dysfunction in ob/ob obese mice.

    PubMed

    Li, Linlin; Hua, Yinan; Dong, Maolong; Li, Quan; Smith, Derek T; Yuan, Ming; Jones, Kyla R; Ren, Jun

    2012-11-01

    Lenalidomide is a potent immunomodulatory agent capable of downregulating proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) and upregulating anti-inflammatory cytokines. Lenalidomide has been shown to elicit cardiovascular effects, although its impact on cardiac function remains obscure. This study was designed to examine the effect of lenalidomide on cardiac contractile function in ob/ob obese mice. C57BL lean and ob/ob obese mice were given lenalidomide (50 mg/kg/day, p.o.) for 3 days. Body fat composition was assessed by dual-energy X-ray absorptiometry. Cardiomyocyte contractile and intracellular Ca(2+) properties were evaluated. Expression of TNF-α, interleukin-6 (IL-6), Fas, Fas ligand (FasL), the short-chain fatty acid receptor GPR41, the NFκB regulator IκB, endoplasmic reticulum (ER) stress, the apoptotic protein markers Bax, Bcl-2, caspase-8, tBid, cytosolic cytochrome C, and caspase-12; and the stress signaling molecules p38 and extracellular signal-regulated kinase (ERK) were evaluated by western blot. ob/ob mice displayed elevated serum TNF-α and IL-6 levels, fat composition and glucose intolerance, the effects of which except glucose intolerance and fat composition were attenuated by lenalidomide. Cardiomyocytes from ob/ob mice exhibited depressed peak shortening (PS) and maximal velocity of shortening/relengthening, prolonged time-to-PS and time-to-90% relengthening as well as intracellular Ca(2+) mishandling, which were ablated by lenalidomide. Western blot analysis revealed elevated levels of TNF-α, IL-6, Fas, Bip, Bax, caspase-8, tBid, cleaved caspase-3 caspase-12, cytochrome C, phosphorylation of p38, and ERK in ob/ob mouse hearts, the effects of which with the exception of Bip, Bax, and caspase-12 were alleviated by lenalidomide. Taken together, these data suggest that lenalidomide is protective against obesity-induced cardiomyopathy possibly through antagonism of cytokine/Fas-induced activation of stress signaling and

  19. Mouth of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  20. Mouth of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  1. Selective T-Type Calcium Channel Blockade Alleviates Hyperalgesia in ob/ob Mice

    PubMed Central

    Latham, Janelle R.; Pathirathna, Sriyani; Jagodic, Miljen M.; Joo Choe, Won; Levin, Michaela E.; Nelson, Michael T.; Yong Lee, Woo; Krishnan, Kathiresan; Covey, Douglas F.; Todorovic, Slobodan M.; Jevtovic-Todorovic, Vesna

    2009-01-01

    OBJECTIVE Morbid obesity may be accompanied by diabetes and painful diabetic neuropathy, a poorly understood condition that is manifested by mechanical or thermal allodynia and hyperalgesia. Recent studies have highlighted the importance of T-type calcium channels (T-channels) in peripheral nociception; therefore, our goal was to examine the function of these channels in the pathophysiology and development of painful diabetic neuropathy. RESEARCH DESIGN AND METHODS In vivo testing of mechanical and thermal sensation, morphometric peripheral nerve studies, and electrophysiological and biochemical measurements were used to characterize the role of T-channels and the development of painful diabetic neuropathy in leptin-deficient (ob/ob) mice. RESULTS We found that ob/ob mice developed significant mechanical and thermal hypersensitivity early in life that coincided with hyperglycemia and was readily reversed with insulin therapy. These disturbances were accompanied by significant biophysical and biochemical modulation of T-channels in dorsal root ganglion neurons as measured by a large increase in the amplitude of T-currents and the expression of mRNA. The most prevalent subtype, α1H (Cav3.2), was most strongly affected. Moreover, (3β,5α,17β)-17-hydroxyestrane-3-carbonitrile (ECN), a novel neuroactive steroid and selective T-channel antagonist, provided dose-dependent alleviation of neuropathic thermal and mechanical hypersensitivity in diabetic ob/ob mice. CONCLUSIONS Our results indicate that pharmacological antagonism of T-channels is potentially an important novel therapeutic approach for the management of painful diabetic neuropathy. PMID:19651818

  2. Enhanced Hypertrophy In ob/ob Mice Due To An Impairment in Expression Of Atrial Natriuretic Peptide

    PubMed Central

    Mascareno, Eduardo; Beckles, Daniel; Dhar-Mascareno, Manya; Siddiqui, M.A.Q.

    2009-01-01

    Rationale We investigated the molecular mechanism(s) that play a role in leptin signaling during the development of left ventricular hypertrophy (LVH) due to pressure overload. To this end, ob/ob leptin deficient and C57BL/6J control mice were subjected transverse aortic constriction (TAC). Methods Control sham C57BL/6J and ob/ob mice, along with C57BL/6J and ob/ob leptin deficient mice were subjected transverse aortic constriction (TAC) for 15 days and then evaluated for morphological, physiological, and molecular changes associated with pressure overload hypertrophy. Results Evaluation by echocardiography revealed a significant increase in left ventricular mass (LVmass) and wall thickness in ob/ob mice subjected to transverse aortic constriction (TAC) as compared to C57BL/6J. Analysis of the expression of molecular markers of LVH, such as atrial natriuretic peptide (ANP), revealed a blunted increase in the level of ANP in ob/ob mice as compared to C57BL/6J mice. We observed that leptin plays a role in modulating the transcriptional activity of the promoter of the ANP gene. Leptin acts by regulating NFATc4, a member of the nuclear factor activated T cell (NFAT) family of transcription factors in cardiomyocytes. Our in vivo studies revealed that ob/ob mice subjected to TAC failed to activate the NFATc4 in the heart, however, intraperitoneal injection of leptin in ob/ob mice restored the NFATc4 DNA-binding activity and induced expression of the ANP gene. Conclusion This study establishes the role of leptin as an anti-hypertrophic agent during pressure overload hypertrophy, and suggests that a key molecular event is the leptin mediated activation of NFATc4 that regulates the transcriptional activation of the ANP gene promoter. PMID:19560554

  3. Enhanced hypertrophy in ob/ob mice due to an impairment in expression of atrial natriuretic peptide.

    PubMed

    Mascareno, Eduardo; Beckles, Daniel; Dhar-Mascareno, Manya; Siddiqui, M A Q

    2009-01-01

    We investigated the molecular mechanism(s) that play a role in leptin signaling during the development of left ventricular hypertrophy (LVH) due to pressure overload. To this end, ob/ob leptin deficient and C57BL/6J control mice were subjected transverse aortic constriction (TAC). Control sham C57BL/6J and ob/ob mice, along with C57BL/6J and ob/ob leptin deficient mice were subjected transverse aortic constriction (TAC) for 15 days and then evaluated for morphological, physiological, and molecular changes associated with pressure overload hypertrophy. Evaluation by echocardiography revealed a significant increase in left ventricular mass (LVmass) and wall thickness in ob/ob mice subjected to transverse aortic constriction (TAC) as compared to C57BL/6J. Analysis of the expression of molecular markers of LVH, such as atrial natriuretic peptide (ANP), revealed a blunted increase in the level of ANP in ob/ob mice as compared to C57BL/6J mice. We observed that leptin plays a role in modulating the transcriptional activity of the promoter of the ANP gene. Leptin acts by regulating NFATc4, a member of the nuclear factor activated T cell (NFAT) family of transcription factors in cardiomyocytes. Our in vivo studies revealed that ob/ob mice subjected to TAC failed to activate the NFATc4 in the heart, however, intraperitoneal injection of leptin in ob/ob mice restored the NFATc4 DNA-binding activity and induced expression of the ANP gene. This study establishes the role of leptin as an anti-hypertrophic agent during pressure overload hypertrophy, and suggests that a key molecular event is the leptin mediated activation of NFATc4 that regulates the transcriptional activation of the ANP gene promoter.

  4. A very low carbohydrate ketogenic diet improves glucose tolerance in ob/ob mice independently of weight loss.

    PubMed

    Badman, Michael K; Kennedy, Adam R; Adams, Andrew C; Pissios, Pavlos; Maratos-Flier, Eleftheria

    2009-11-01

    In mice of normal weight and with diet-induced obesity, a high-fat, low-carbohydrate ketogenic diet (KD) causes weight loss, reduced circulating glucose and lipids, and dramatic changes in hepatic gene expression. Many of the effects of KD are mediated by fibroblast growth factor 21 (FGF21). We tested the effects of KD feeding on ob/ob mice to determine if metabolic effects would occur in obesity secondarily to leptin deficiency. We evaluated the effect of prolonged KD feeding on weight, energy homeostasis, circulating metabolites, glucose homeostasis, and gene expression. Subsequently, we evaluated the effects of leptin and fasting on FGF21 expression in ob/ob mice. KD feeding of ob/ob mice normalized fasting glycemia and substantially reduced insulin and lipid levels in the absence of weight loss. KD feeding was associated with significant increases in lipid oxidative genes and reduced expression of lipid synthetic genes, including stearoyl-coenzyme A desaturase 1, but no change in expression of inflammatory markers. In chow-fed ob/ob mice, FGF21 mRNA was elevated 10-fold compared with wild-type animals, and no increase from this elevated baseline was seen with KD feeding. Administration of leptin to chow-fed ob/ob mice led to a 24-fold induction of FGF21. Fasting also induced hepatic FGF21 in ob/ob mice. Thus, KD feeding improved ob/ob mouse glucose homeostasis without weight loss or altered caloric intake. These data demonstrate that manipulation of dietary macronutrient composition can lead to marked improvements in metabolic profile of leptin-deficient obese mice in the absence of weight loss.

  5. Flooding of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    A mixture of heavy rainfall, snowmelt, and ice jams in late May and early June of this year caused the Ob River and surrounding tributaries in Western Siberia to overflow their banks. The flooding can be seen in thess image taken on June 16, 2002, by the MODIS (Moderate Resolution Imaging Spectroradiometer) instrument aboard the Terra satellite. Last year, the river flooded farther north. Normally, the river resembles a thin black line, but floods have swollen the river considerably. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  6. Flooding of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    A mixture of heavy rainfall, snowmelt, and ice jams in late May and early June of this year caused the Ob River and surrounding tributaries in Western Siberia to overflow their banks. The flooding can be seen in thess image taken on June 16, 2002, by the MODIS (Moderate Resolution Imaging Spectroradiometer) instrument aboard the Terra satellite. Last year, the river flooded farther north. Normally, the river resembles a thin black line, but floods have swollen the river considerably. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  7. Dietary glucose increases plasma insulin and decreases brown adipose tissue thermogenic activity in adrenalectomized ob/ob mice.

    PubMed

    Nei, Y M; Romsos, D R

    1991-09-01

    The purpose of this study was to determine whether consumption of a high glucose diet would increase plasma insulin concentrations and decrease brown adipose tissue metabolism in adrenalectomized ob/ob mice previously fed a high starch diet. Male sham-operated and adrenalectomized ob/ob and lean mice were fed a high starch diet for 12 d, then switched to a high glucose diet for the last 2 or 4 d of the 14- or 16-d feeding trials. Adrenalectomized ob/ob mice consumed 16% more energy and gained 50% more weight without an increase in oxygen consumption when switched from a high starch diet to a high glucose diet. Within 2 d after the switch to the high glucose diet, plasma insulin concentrations increased by 70% without any change in plasma glucose concentrations; brown adipose tissue metabolism, as assessed by GDP binding to brown adipose tissue mitochondria, was decreased by 26% 4 d after the diet switch. Sham-operated ob/ob and lean mice and adrenalectomized lean mice were minimally affected by the switch to the high glucose diet. The increase in plasma insulin concentrations in adrenalectomized ob/ob mice induced by the high glucose diet may contribute to the observed depression in brown adipose tissue metabolism.

  8. Hormone-sensitive lipase deficiency suppresses insulin secretion from pancreatic islets of Lep{sup ob/ob} mice

    SciTech Connect

    Sekiya, Motohiro; Yahagi, Naoya; Tamura, Yoshiaki; Okazaki, Hiroaki; Igarashi, Masaki; Ohta, Keisuke; Takanashi, Mikio; Kumagai, Masayoshi; Takase, Satoru; Nishi, Makiko; Takeuchi, Yoshinori; Izumida, Yoshihiko; Kubota, Midori; Ohashi, Ken; Iizuka, Yoko; Yagyu, Hiroaki; Gotoda, Takanari; Nagai, Ryozo; Shimano, Hitoshi; Yamada, Nobuhiro; and others

    2009-09-25

    It has long been a matter of debate whether the hormone-sensitive lipase (HSL)-mediated lipolysis in pancreatic {beta}-cells can affect insulin secretion through the alteration of lipotoxicity. We generated mice lacking both leptin and HSL (Lep{sup ob/ob}/HSL{sup -/-}) and explored the role of HSL in pancreatic {beta}-cells in the setting of obesity. Lep{sup ob/ob}/HSL{sup -/-} developed elevated blood glucose levels and reduced plasma insulin levels compared with Lep{sup ob/ob}/HSL{sup +/+} in a fed state, while the deficiency of HSL did not affect glucose homeostasis in Lep{sup +/+} background. The deficiency of HSL exacerbated the accumulation of triglycerides in Lep{sup ob/ob} islets, leading to reduced glucose-stimulated insulin secretion. The deficiency of HSL also diminished the islet mass in Lep{sup ob/ob} mice due to decreased cell proliferation. In conclusion, HSL affects insulin secretary capacity especially in the setting of obesity.

  9. American ginseng berry juice intake reduces blood glucose and body weight in ob/ob mice.

    PubMed

    Xie, J T; Wang, C Z; Ni, M; Wu, J A; Mehendale, S R; Aung, H H; Foo, A; Yuan, C S

    2007-10-01

    Diabetes is a serious chronic metabolic disease and has a significant impact on patients' lives and the health care system. We previously observed that the organic solvent extract of American ginseng berry possessed significant antidiabetic effects in obese diabetic ob/ob mice after intraperitoneal injection. If American ginseng berry is useful as a dietary supplement, simple preparation and oral intake would be a convenient, safe, and practical means for consumers. In this study, the simply prepared berry juice was first analyzed using high-performance liquid chromatography, and then administered orally in the ob/ob mice. The animals received daily berry juice 0.6 mL/kg or vehicle for 10 consecutive days. The results indicated that oral juice administration significantly lowered fasting blood glucose levels, and this effect continued for at least 10 d after cessation of the treatment. Data from intraperitoneal glucose tolerance test demonstrated that there was a notable improvement in glucose tolerance in the juice treated group. In addition, the berry juice significantly reduced body weight. Our data suggest that ginseng berry juice, as a dietary supplement, may have functional efficacy in consumers with diabetes.

  10. A HIPPARCOS Census of the Nearby OB Associations

    NASA Astrophysics Data System (ADS)

    de Zeeuw, P. T.; Hoogerwerf, R.; de Bruijne, J. H. J.; Brown, A. G. A.; Blaauw, A.

    1999-01-01

    A comprehensive census of the stellar content of the OB associations within 1 kpc from the Sun is presented, based on Hipparcos positions, proper motions, and parallaxes. It is a key part of a long-term project to study the formation, structure, and evolution of nearby young stellar groups and related star-forming regions. OB associations are unbound ``moving groups,'' which can be detected kinematically because of their small internal velocity dispersion. The nearby associations have a large extent on the sky, which traditionally has limited astrometric membership determination to bright stars (V<~6 mag), with spectral types earlier than ~B5. The Hipparcos measurements allow a major improvement in this situation. Moving groups are identified in the Hipparcos Catalog by combining de Bruijne's refurbished convergent point method with the ``Spaghetti method'' of Hoogerwerf & Aguilar. Astrometric members are listed for 12 young stellar groups, out to a distance of ~650 pc. These are the three subgroups Upper Scorpius, Upper Centaurus Lupus, and Lower Centaurus Crux of Sco OB2, as well as Vel OB2, Tr 10, Col 121, Per OB2, alpha Persei (Per OB3), Cas-Tau, Lac OB1, Cep OB2, and a new group in Cepheus, designated as Cep OB6. The selection procedure corrects the list of previously known astrometric and photometric B- and A-type members in these groups and identifies many new members, including a significant number of F stars, as well as evolved stars, e.g., the Wolf-Rayet stars gamma^2 Vel (WR 11) in Vel OB2 and EZ CMa (WR 6) in Col 121, and the classical Cepheid delta Cep in Cep OB6. Membership probabilities are given for all selected stars. Monte Carlo simulations are used to estimate the expected number of interloper field stars. In the nearest associations, notably in Sco OB2, the later-type members include T Tauri objects and other stars in the final pre-main-sequence phase. This provides a firm link between the classical high-mass stellar content and ongoing low

  11. Automated identification of OB associations in M31

    NASA Technical Reports Server (NTRS)

    Magnier, Eugene A.; Battinelli, Paolo; Lewin, Walter H. G.; Haiman, Zoltan; Paradijs, Jan Van; Hasinger, Guenther; Pietsch, Wolfgang; Supper, Rodrigo; Truemper, Joachim

    1993-01-01

    A new identification of OB associations in M31 has been performed using the Path Linkage Criterion (PLC) technique of Battinelli (1991). We found 174 associations with a very small contamination (less than 5%) by random clumps of stars. The expected total number and average size of OB associations in the region of M 31 covered by our data set (Magnier et al. 1992) are approximately 280 and approximately 90 pc, respectively. M31 associations therefore have sizes similar to those of OB associations observed in nearby galaxies, so that we can consider them to be classical OB associations. This list of OB associations will be used for the study of the spatial distribution of OB associations and their correlation with other objects. Taking into account the fact that we do not cover the entire disk of M31, we extrapolate a total number of association in M31 of approximately 420.

  12. Automated identification of OB associations in M31

    NASA Astrophysics Data System (ADS)

    Magnier, Eugene A.; Battinelli, Paolo; Lewin, Walter H. G.; Haiman, Zoltan; van Paradijs, Jan; Hasinger, Guenther; Pietsch, Wolfgang; Supper, Rodrigo; Truemper, Joachim

    1993-10-01

    A new identification of OB associations in M31 has been performed using the Path Linkage Criterion (PLC) technique of Battinelli (1991). We found 174 associations with a very small contamination (less than 5%) by random clumps of stars. The expected total number and average size of OB associations in the region of M 31 covered by our data set (Magnier et al. 1992) are approximately 280 and approximately 90 pc, respectively. M31 associations therefore have sizes similar to those of OB associations observed in nearby galaxies, so that we can consider them to be classical OB associations. This list of OB associations will be used for the study of the spatial distribution of OB associations and their correlation with other objects. Taking into account the fact that we do not cover the entire disk of M31, we extrapolate a total number of association in M31 of approximately 420.

  13. Automated identification of OB associations in M31

    NASA Technical Reports Server (NTRS)

    Magnier, Eugene A.; Battinelli, Paolo; Lewin, Walter H. G.; Haiman, Zoltan; Paradijs, Jan Van; Hasinger, Guenther; Pietsch, Wolfgang; Supper, Rodrigo; Truemper, Joachim

    1993-01-01

    A new identification of OB associations in M31 has been performed using the Path Linkage Criterion (PLC) technique of Battinelli (1991). We found 174 associations with a very small contamination (less than 5%) by random clumps of stars. The expected total number and average size of OB associations in the region of M 31 covered by our data set (Magnier et al. 1992) are approximately 280 and approximately 90 pc, respectively. M31 associations therefore have sizes similar to those of OB associations observed in nearby galaxies, so that we can consider them to be classical OB associations. This list of OB associations will be used for the study of the spatial distribution of OB associations and their correlation with other objects. Taking into account the fact that we do not cover the entire disk of M31, we extrapolate a total number of association in M31 of approximately 420.

  14. Multicolor Polarization Study of ARA OB1

    NASA Astrophysics Data System (ADS)

    Waldhausen, Silvia; Martínez, Ruben E.; Feinstein, Carlos

    1999-06-01

    We present the results of a multicolor polarimetric study of stars in the association Ara OB1. Several objects belonging to the clusters NGC 6204, 6193, and 6167 and the local field were observed as part of a global study of the region. The polarimetry shows that the orientation of the polarimetric vectors of each star is very similar within each cluster. The average values are 35.5d+/-15.1d and 52.2d+/-16 deg for NGC 6204 and 6193, respectively. An average value of 106.5d+/-9.9d is found for NGC 6167, but the angle distribution is asymmetric, and a second component can be fitted to the angle histogram (P.A.~120.8d+/-11.6d), showing a behavior not observed in NGC 6204 and 6193. So, we suggest that some of the observed stars perhaps belong to another stars grouping, located behind NGC 6167 and between a dust layer with a different orientation of the grains. The large difference in the polarization angle between NGC 6167 and the other two clusters could also be explained because NGC 6167 is supposed to be at the center of a gas-expanding structure and it is possible that this cluster was the origin of the star formation process in the Ara OB1 association, triggered by stellar winds or supernova explosions.

  15. New style OBS to the abyss

    NASA Astrophysics Data System (ADS)

    Shiobara, H.; Ito, A.; Sugioka, H.; Shinohara, M.

    2013-12-01

    Generally, we have been trying to widen observation ranges at the seafloor in multi dimensions, such as term of observation, period, area and strength of signal. One of them is a development of the ocean bottom seismometer (OBS) usable even at the bottom of the trench, which has been a target since 1989. Several trials encountered some troubles that would come from the mechanical design and parts used in our standard OBS. To clear this problem, we developed the New design Ultra Deep OBS (NUDOBS) and have started test observations. This OBS is a completely new concept design that can be deployed up to 10000 m depth without using any underwater cables and the traditional anchor releasing system. Its maximum observation term is about one year. The NUDOBS equips 3-component omni-directional geophones (15 Hz) with no gimbal and the 3-axis MEMS accelerometer. The 6-ch data recorder for OBS (LS9100-T6, Hakusan), 21 DD size Li-cells are installed in the titanium pressure case (Ø200×H490) with these sensors. The main point of the NUDOBS lies at the 3-stage mechanical action, the deployment stage, the observation stage and the recovery stage. The newly developed acoustic transponder that enables the two-step action controls transitions between stages twice. To reduce the noise by the bottom current, there is no mechanical coupling of the pressure case with other parts except a thin umbilical rope during the observation stage. The whole system of the NUDOBS is like as a small mooring buoy using 6 glass-sphere floats for the deep water (~12000 m). The deployment was performed to release these floats, the 20-m rope and the main unit of the NUDOBS at last. The descending speed is about 80 m/min. The NUDOBS, although it is still a prototype, has been tested twice. The first one was at the Philippine Sea site (4900 m depth) with other instruments those requires the ROV from Nov. 2012 until Feb. 2013. In this observation, we could watch two transitions of the NUDOBS by video

  16. Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob/ob Mice

    PubMed Central

    Sáinz, Neira; Rodríguez, Amaia; Catalán, Victoria; Becerril, Sara; Ramírez, Beatriz; Gómez-Ambrosi, Javier; Frühbeck, Gema

    2010-01-01

    Obese leptin-deficient ob/ob mice exhibit a low-grade chronic inflammation together with a low muscle mass. Our aim was to analyze the changes in muscle expression levels of genes related to oxidative stress and inflammatory responses in leptin deficiency and to identify the effect of in vivo leptin administration. Ob/ob mice were divided in three groups as follows: control ob/ob, leptin-treated ob/ob (1 mg/kg/d) and leptin pair-fed ob/ob mice. Gastrocnemius weight was lower in control ob/ob than in wild type mice (P < .01) exhibiting an increase after leptin treatment compared to control and pair-fed (P < .01) ob/ob animals. Thiobarbituric acid reactive substances, markers of oxidative stress, were higher in serum (P < .01) and gastrocnemius (P = .05) of control ob/ob than in wild type mice and were significantly decreased (P < .01) by leptin treatment. Leptin deficiency altered the expression of 1,546 genes, while leptin treatment modified the regulation of 1,127 genes with 86 of them being involved in oxidative stress, immune defense and inflammatory response. Leptin administration decreased the high expression of Crybb1, Hspb3, Hspb7, Mt4, Cat, Rbm9, Serpinc1 and Serpinb1a observed in control ob/ob mice, indicating that it improves inflammation and muscle loss. PMID:20671928

  17. Effects of AMPK activation on insulin sensitivity and metabolism in leptin-deficient ob/ob mice.

    PubMed

    Zachariah Tom, Robby; Garcia-Roves, Pablo M; Sjögren, Rasmus J O; Jiang, Lake Q; Holmström, Maria H; Deshmukh, Atul S; Vieira, Elaine; Chibalin, Alexander V; Björnholm, Marie; Zierath, Juleen R

    2014-05-01

    AMP-activated protein kinase (AMPK) is a heterotrimeric complex, composed of a catalytic subunit (α) and two regulatory subunits (β and γ), which act as a metabolic sensor to regulate glucose and lipid metabolism. A mutation in the γ3 subunit (AMPKγ3(R225Q)) increases basal AMPK phosphorylation, while concomitantly reducing sensitivity to AMP. AMPKγ3(R225Q) (γ3(R225Q)) transgenic mice are protected against dietary-induced triglyceride accumulation and insulin resistance. We determined whether skeletal muscle-specific expression of AMPKγ3(R225Q) prevents metabolic abnormalities in leptin-deficient ob/ob (ob/ob-γ3(R225Q)) mice. Glycogen content was increased, triglyceride content was decreased, and diacylglycerol and ceramide content were unaltered in gastrocnemius muscle from ob/ob-γ3(R225Q) mice, whereas glucose tolerance was unaltered. Insulin-stimulated glucose uptake in extensor digitorum longus muscle during the euglycemic-hyperinsulinemic clamp was increased in lean γ3(R225Q) mice, but not in ob/ob-γ3(R225Q) mice. Acetyl-CoA carboxylase phosphorylation was increased in gastrocnemius muscle from γ3(R225Q) mutant mice independent of adiposity. Glycogen and triglyceride content were decreased after leptin treatment (5 days) in ob/ob mice, but not in ob/ob-γ3(R225Q) mice. In conclusion, metabolic improvements arising from muscle-specific expression of AMPKγ3(R225Q) are insufficient to ameliorate insulin resistance and obesity in leptin-deficient mice. Central defects due to leptin deficiency may override any metabolic benefit conferred by peripheral overexpression of the AMPKγ3(R225Q) mutation.

  18. Localization of the human OB gene (OBS) to chromosome 7q32 by fluorescence in situ hybridization

    SciTech Connect

    Geffroy, S.; Duban, B.; Martinville, B. de

    1995-08-10

    An important gene involved in the pathogenesis of obesity is the product of the human homologue of the murine obese gene (gene symbol OBS). Using fluorescence in situ hybridization (FISH), we have localized the human OB gene to human chromosome 7, specifically to region 7q32.1. The FISH data of human OBS provide a gene-associated marker for genetic mapping. 8 refs., 1 fig.

  19. The origin of OB runaway stars.

    PubMed

    Fujii, Michiko S; Portegies Zwart, Simon

    2011-12-09

    About 20% of all massive stars in the Milky Way have unusually high velocities, the origin of which has puzzled astronomers for half a century. We argue that these velocities originate from strong gravitational interactions between single stars and binaries in the centers of star clusters. The ejecting binary forms naturally during the collapse of a young (≤1 million years old) star cluster. This model replicates the key characteristics of OB runaways in our galaxy, and it explains the presence of runaway stars of ≥100 solar masses (M(⊙)) around young star clusters, such as R136 and Westerlund 2. The high proportion and the distributions in mass and velocity of runaways in the Milky Way are reproduced if the majority of massive stars are born in dense and relatively low-mass (5000 to 10,000 M(⊙)) clusters.

  20. The Origin of OB Runaway Stars

    NASA Astrophysics Data System (ADS)

    Fujii, Michiko S.; Portegies Zwart, Simon

    2011-12-01

    About 20% of all massive stars in the Milky Way have unusually high velocities, the origin of which has puzzled astronomers for half a century. We argue that these velocities originate from strong gravitational interactions between single stars and binaries in the centers of star clusters. The ejecting binary forms naturally during the collapse of a young (≲1 milion years old) star cluster. This model replicates the key characteristics of OB runaways in our galaxy, and it explains the presence of runaway stars of ≳100 solar masses (M⊙) around young star clusters, such as R136 and Westerlund 2. The high proportion and the distributions in mass and velocity of runaways in the Milky Way are reproduced if the majority of massive stars are born in dense and relatively low-mass (5000 to 10,000 M⊙) clusters.

  1. Open clusters in Auriga OB2

    NASA Astrophysics Data System (ADS)

    Marco, Amparo; Negueruela, Ignacio

    2016-06-01

    We study the area around the H II region Sh 2-234, including the young open cluster Stock 8, to investigate the extent and definition of the association Aur OB2 and the possible role of triggering in massive cluster formation. We obtained Strömgren and J, H, KS photometry for Stock 8 and Strömgren photometry for two other cluster candidates in the area, which we confirm as young open clusters and name Alicante 11 and Alicante 12. We took spectroscopy of ˜33 early-type stars in the area, including the brightest cluster members. We calculate a common distance of 2.80^{+0.27}_{-0.24} kpc for the three open clusters and surrounding association. We derive an age 4-6 Ma for Stock 8, and do not find a significantly different age for the other clusters or the association. The star LS V +34°23, with spectral type O8 II(f), is likely the main source of ionization of Sh 2-234. We observe an important population of pre-main-sequence stars, some of them with discs, associated with the B-type members lying on the main sequence. We interpret the region as an area of recent star formation with some residual and very localized ongoing star formation. We do not find evidence for sequential star formation on a large scale. The classical definition of Aur OB2 has to be reconsidered, because its two main open clusters, Stock 8 and NGC 1893, are not at the same distance. Stock 8 is probably located in the Perseus arm, but other nearby H II regions whose distances also place them in this arm show quite different distances and radial velocities and, therefore, are not connected.

  2. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers

  3. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers

  4. Odor-Induced Neuronal Rhythms in the Olfactory Bulb Are Profoundly Modified in ob/ob Obese Mice

    PubMed Central

    Chelminski, Yan; Magnan, Christophe; Luquet, Serge H.; Everard, Amandine; Meunier, Nicolas; Gurden, Hirac; Martin, Claire

    2017-01-01

    Leptin, the product of the Ob(Lep) gene, is a peptide hormone that plays a major role in maintaining the balance between food intake and energy expenditure. In the brain, leptin receptors are expressed by hypothalamic cells but also in the olfactory bulb, the first central structure coding for odors, suggesting a precise function of this hormone in odor-evoked activities. Although olfaction plays a key role in feeding behavior, the ability of the olfactory bulb to integrate the energy-related signal leptin is still missing. Therefore, we studied the fate of odor-induced activity in the olfactory bulb in the genetic context of leptin deficiency using the obese ob/ob mice. By means of an odor discrimination task with concomitant local field potential recordings, we showed that ob/ob mice perform better than wild-type (WT) mice in the early stage of the task. This behavioral gain of function was associated in parallel with profound changes in neuronal oscillations in the olfactory bulb. The distribution of the peaks in the gamma frequency range was shifted toward higher frequencies in ob/ob mice compared to WT mice before learning. More notably, beta oscillatory activity, which has been shown previously to be correlated with olfactory discrimination learning, was longer and stronger in expert ob/ob mice after learning. Since oscillations in the olfactory bulb emerge from mitral to granule cell interactions, our results suggest that cellular dynamics in the olfactory bulb are deeply modified in ob/ob mice in the context of olfactory learning. PMID:28154537

  5. Effect of Leptin Replacement on PCSK9 in ob/ob Mice and Female Lipodystrophic Patients

    PubMed Central

    Levenson, Amy E.; Haas, Mary E.; Miao, Ji; Brown, Rebecca J.; de Ferranti, Sarah D.; Muniyappa, Ranganath

    2016-01-01

    Leptin treatment has beneficial effects on plasma lipids in patients with lipodystrophy, but the underlying mechanism is unknown. Proprotein convertase subtilisin/kexin type 9 (PCSK9) decreases low-density lipoprotein (LDL) clearance, promotes hypercholesterolemia, and has recently emerged as a novel therapeutic target. To determine the effect of leptin on PCSK9, we treated male and female ob/ob mice with leptin for 4 days via sc osmotic pumps (∼24 μg/d). Leptin reduced body weight and food intake in all mice, but the effects of leptin on plasma PCSK9 and lipids differed markedly between the sexes. In male mice, leptin suppressed PCSK9 but had no effect on plasma triglycerides or cholesterol. In female mice, leptin suppressed plasma triglycerides and cholesterol but had no effect on plasma PCSK9. In parallel, we treated female lipodystrophic patients (8 females, ages 5–23 y) with sc metreleptin injections (∼4.4 mg/d) for 4–6 months. In this case, leptin reduced plasma PCSK9 by 26% (298 ± 109 vs 221 ± 102 ng/mL; n = 8; P = .008), and the change in PCSK9 was correlated with a decrease in LDL cholesterol (r2 = 0.564, P = .03). In summary, in leptin-deficient ob/ob mice, the effects of leptin on PCSK9 and plasma lipids appeared to be independent of one another and strongly modified by sex. On the other hand, in lipodystrophic females, leptin treatment reduced plasma PCSK9 in parallel with LDL cholesterol. PMID:26824363

  6. Effect of Leptin Replacement on PCSK9 in ob/ob Mice and Female Lipodystrophic Patients.

    PubMed

    Levenson, Amy E; Haas, Mary E; Miao, Ji; Brown, Rebecca J; de Ferranti, Sarah D; Muniyappa, Ranganath; Biddinger, Sudha B

    2016-04-01

    Leptin treatment has beneficial effects on plasma lipids in patients with lipodystrophy, but the underlying mechanism is unknown. Proprotein convertase subtilisin/kexin type 9 (PCSK9) decreases low-density lipoprotein (LDL) clearance, promotes hypercholesterolemia, and has recently emerged as a novel therapeutic target. To determine the effect of leptin on PCSK9, we treated male and female ob/ob mice with leptin for 4 days via sc osmotic pumps (∼24 μg/d). Leptin reduced body weight and food intake in all mice, but the effects of leptin on plasma PCSK9 and lipids differed markedly between the sexes. In male mice, leptin suppressed PCSK9 but had no effect on plasma triglycerides or cholesterol. In female mice, leptin suppressed plasma triglycerides and cholesterol but had no effect on plasma PCSK9. In parallel, we treated female lipodystrophic patients (8 females, ages 5-23 y) with sc metreleptin injections (∼4.4 mg/d) for 4-6 months. In this case, leptin reduced plasma PCSK9 by 26% (298 ± 109 vs 221 ± 102 ng/mL; n = 8; P = .008), and the change in PCSK9 was correlated with a decrease in LDL cholesterol (r(2) = 0.564, P = .03). In summary, in leptin-deficient ob/ob mice, the effects of leptin on PCSK9 and plasma lipids appeared to be independent of one another and strongly modified by sex. On the other hand, in lipodystrophic females, leptin treatment reduced plasma PCSK9 in parallel with LDL cholesterol.

  7. Photometry of stars in the Cas OB5 Associations

    NASA Astrophysics Data System (ADS)

    Tanriver, Mehmet; Keskin, Ahmet

    2016-07-01

    OB associations are a grouping of very young associations, contain 10-100 very hot massive stars, spectral types O and B. Also, the OB associations contain low and intermediate mass stars, too. Association members are believed to form within the same small volume inside a giant molecular cloud. Once the surrounding dust and gas is blown away, the remaining stars become not tied up and begin to drift separately. It is believed that the majority of all stars in the Milky Way were formed in OB associations. O type stars are short-lived, and will be at an end as supernovae after roundly a million years. OB associations are generally only a few million years in age or less. In this study, the photometry of UU Cas and field star which been Cas OB5 association member was carried out. Light curves and color diagrams are given in the study.

  8. Anti-obesity and anti-diabetic effects of a standardized potato extract in ob/ob mice

    PubMed Central

    KU, SAE KWANG; SUNG, SOO HYUN; CHOUNG, JAI JUN; CHOI, JAE-SUK; SHIN, YONG KOOK; KIM, JOO WAN

    2016-01-01

    The potato (Solanum tuberosum) has been cultivated globally for food for millenia. Potato contains proteinase inhibitor II, which catalyzes the release of cholecystokinin (CCK), leading to delayed gastric emptying in humans. The present study investigated the anti-obesity effects of Slendesta™ Potato Extract (SLD), a standardized potato extract containing 5% proteinase inhibitor II, in the ob/ob obese mice. Three doses of SLD (50, 150 or 300 mg/kg) were orally administered to ob/ob mice once a day for 28 days, whereas control mice were administered distilled water. Four weeks after SLD treatment, the changes in body weight, food consumption, epididymal fat weight, serum chemistry, insulin, leptin and adiponectin contents, and fat histopathology were determined and compared with ob/ob mice treated with 750 mg/kg conjugate linoleic acid. As a result of SLD treatment in the obese mice, body weight, food consumption, epididymal fat, serum biochemistry, histomorphological changes of fat and pancreas were significantly and dose-dependently decreased compared with ob/ob control mice. These obesity and type 2 diabetes associated alterations were significantly inhibited after SLD treatment for 28 days. Thus, the present results indicate that SLD has potential as an alternative therapeutic agent for obesity. PMID:27347062

  9. Leptin Increases Striatal Dopamine D2 Receptor Binding in Leptin-Deficient Obese (ob/ob) Mice

    SciTech Connect

    Pfaffly, J.; Michaelides, M.; Wang, G-J.; Pessin, J.E.; Volkow, N.D.; Thanos, P.K.

    2010-06-01

    Peripheral and central leptin administration have been shown to mediate central dopamine (DA) signaling. Leptin-receptor deficient rodents show decreased DA D2 receptor (D2R) binding in striatum and unique DA profiles compared to controls. Leptin-deficient mice show increased DA activity in reward-related brain regions. The objective of this study was to examine whether basal D2R-binding differences contribute to the phenotypic behaviors of leptin-deficient ob/ob mice, and whether D2R binding is altered in response to peripheral leptin treatment in these mice. Leptin decreased body weight, food intake, and plasma insulin concentration in ob/ob mice but not in wild-type mice. Basal striatal D2R binding (measured with autoradiography [{sup 3}H] spiperone) did not differ between ob/ob and wild-type mice but the response to leptin did. In wild-type mice, leptin decreased striatal D2R binding, whereas, in ob/ob mice, leptin increased D2R binding. Our findings provide further evidence that leptin modulates D2R expression in striatum and that these effects are genotype/phenotype dependent.

  10. OB protein binds specifically to the choroid plexus of mice and rats.

    PubMed

    Devos, R; Richards, J G; Campfield, L A; Tartaglia, L A; Guisez, Y; van der Heyden, J; Travernier, J; Plaetinck, G; Burn, P

    1996-05-28

    Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor.

  11. Apolipoprotein A-I gene transfer exerts immunomodulatory effects and reduces vascular inflammation and fibrosis in ob/ob mice.

    PubMed

    Spillmann, Frank; De Geest, Bart; Muthuramu, Ilayaraja; Amin, Ruhul; Miteva, Kapka; Pieske, Burkert; Tschöpe, Carsten; Van Linthout, Sophie

    2016-01-01

    Obesity is associated with vascular inflammation, fibrosis and reduced high-density lipoproteins (HDL)-cholesterol. We aimed to investigate whether adenoviral gene transfer with human apolipoprotein (apo) A-I (Ad.A-I), the main apo of HDL, could exert immunomodulatory effects and counteract vascular inflammation and fibrosis in ob/ob mice. Ad.A-I transfer was performed in 8 weeks (w) old ob/ob mice, which were sacrificed 7 w later. The aorta was excised for mRNA analysis and the spleen for splenocyte isolation for subsequent flow cytometry and co-culture with murine fibroblasts. HDL was added to mononuclear cells (MNC) and fibroblasts to assess their impact on adhesion capacity and collagen deposition, respectively. Ad.A-I led to a 1.8-fold (p < 0.05) increase in HDL-cholesterol versus control ob/ob mice at the day of sacrifice, which was paralleled by a decrease in aortic TNF-α and VCAM-1 mRNA expression. Pre-culture of MNC with HDL decreased their adhesion to TNF-α-activated HAEC. Ad.A-I exerted immunomodulatory effects as evidenced by a downregulation of aortic NOD2 and NLRP3 mRNA expression and by a 12 %, 6.9 %, and 15 % decrease of the induced proliferation/activity of total splenic MNC, CD4+, and CD8+ cells in ob/ob Ad.A-I versus control ob/ob mice, respectively (p < 0.05). Ad.A-I further reduced aortic collagen I and III mRNA expression by 62 % and 66 %, respectively (p < 0.0005), and abrogated the potential of ob/ob splenocytes to induce the collagen content in murine fibroblasts upon co-culture. Finally, HDL decreased the TGF-ß1-induced collagen deposition of murine fibroblasts in vitro. Apo A-I transfer counteracts vascular inflammation and fibrosis in ob/ob mice.

  12. PPARα-Deficient ob/ob Obese Mice Become More Obese and Manifest Severe Hepatic Steatosis Due to Decreased Fatty Acid Oxidation.

    PubMed

    Gao, Qian; Jia, Yuzhi; Yang, Gongshe; Zhang, Xiaohong; Boddu, Prajwal C; Petersen, Bryon; Narsingam, Saiprasad; Zhu, Yi-Jun; Thimmapaya, Bayar; Kanwar, Yashpal S; Reddy, Janardan K

    2015-05-01

    Obesity poses an increased risk of developing metabolic syndrome and closely associated nonalcoholic fatty liver disease, including liver cancer. Satiety hormone leptin-deficient (ob/ob) mice, considered paradigmatic of nutritional obesity, develop hepatic steatosis but are less prone to developing liver tumors. Sustained activation of peroxisome proliferator-activated receptor α (PPARα) in ob/ob mouse liver increases fatty acid oxidation (FAO), which contributes to attenuation of obesity but enhances liver cancer risk. To further evaluate the role of PPARα-regulated hepatic FAO and energy burning in the progression of fatty liver disease, we generated PPARα-deficient ob/ob (PPARα(Δ)ob/ob) mice. These mice become strikingly more obese compared to ob/ob littermates, with increased white and brown adipose tissue content and severe hepatic steatosis. Hepatic steatosis becomes more severe in fasted PPARα(Δ)ob/ob mice as they fail to up-regulate FAO systems. PPARα(Δ)ob/ob mice also do not respond to peroxisome proliferative and mitogenic effects of PPARα agonist Wy-14,643. Although PPARα(Δ)ob/ob mice are severely obese, there was no significant increase in liver tumor incidence, even when maintained on a diet containing Wy-14,643. We conclude that sustained PPARα activation-related increase in FAO in fatty livers of obese ob/ob mice increases liver cancer risk, whereas deletion of PPARα in ob/ob mice aggravates obesity and hepatic steatosis. However, it does not lead to liver tumor development because of reduction in FAO and energy burning. Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

  13. A deep PSPC obervation of the Cyg OB2 association

    NASA Technical Reports Server (NTRS)

    Waldron, Wayne

    1995-01-01

    The Cyg OB2 Association contains six of the fifteen most luminous stars in the Galaxy as well as the most luminous and most heavily reddened Galactic star, Cyg OB2 #12. In addition, the association contains a number of Wolf-Rayet stars, at least one early-type binary (Cyg OB2 #5), and a trapezium-like system (Cyg OB2 #8). Though not physically associated with the association, the X-ray source Cyg X-3 lies within a half degree of the center of the association. The association is a known source of X-rays. The X-ray emission from this region includes a contribution from Cyg X-3, as well as coronal emission from OB stars and hot diffuse gas. The region was extensively sampled by the HEAO-2 IPC (136 ks, which includes a single pointing of 57 ks on the central region of the association). A 3.6 ks PSPC observation of this region was obtained in the AO1 pointing phase. In addition, the four brightest OB stars (Cyg OB2 #5, #8A, #9, and #12) have been extensively monitored with the VLA. These stars show evidence of strong radio variability, and nonthermal radio emission characteristics. Several of these radio observations were taken within about a month of an IPC observation, and the two PSPC observations. We obtained a 19.5 ks exposure of the Cyg OB2 Association using the ROSAT PSPC instrument. Scientific tasks included: (1) extracting the PSPC spectral distributions of the brightest OB stars; (2) comparing the 19.5 ks spectral data with the 3.6 ks spectral data; (3) investigating the long term X-ray variability (15 years); and (4) comparing the X-ray and radio variability.

  14. New OBS network deployment offshore Ireland

    NASA Astrophysics Data System (ADS)

    Le Pape, Florian; Bean, Chris; Craig, David; Jousset, Philippe; Horan, Clare; Hogg, Colin; Donne, Sarah; McCann, Hannah; Möllhoff, Martin; Kirk, Henning; Ploetz, Aline

    2016-04-01

    With the presence of the stormy NE Atlantic, Ireland is ideally located to investigate further our understanding of ocean generated microseisms and use noise correlation methods to develop seismic imaging in marine environments as well as time-lapse monitoring. In order to study the microseismic activity offshore Ireland, 10 Broad Band Ocean Bottom Seismographs (OBSs) units including hydrophones have been deployed in January 2016 across the shelf offshore Donegal and out into the Rockall Trough. This survey represents the first Broadband passive study in this part of the NE Atlantic. The instruments will be recovered in August 2016 providing 8 months worth of data to study microseisms but also the offshore seismic activity in the area. One of the main goal of the survey is to investigate the spatial and temporal distributions of dominant microseism source regions, close to the microseism sources. Additionally we will study the coupling of seismic and acoustic signals at the sea bed and its evolution in both the deep water and continental shelf areas. Furthermore, the survey also aims to investigate further the relationship between sea state conditions (e.g. wave height, period), seafloor pressure variations and seismic data recorded on both land and seafloor. Finally, the deployed OBS network is also the first ever attempt to closely monitor local offshore earthquakes in Ireland. Ireland seismicity although relatively low can reduce slope stability and poses the possibility of triggering large offshore landslides and local tsunamis.

  15. Additional red and reddened stars in Cyg OB2 association

    NASA Technical Reports Server (NTRS)

    Parthasarathy, M.; Jain, S. K.

    1989-01-01

    Several new red and reddened stars are detected in the most heavily reddened associations Cyg OB2. About 47 IRAS sources are detected in Cyg OB2. Their flux distributions, and colors, suggest that they are young stellar objects embedded in dust envelopes or disks (some of them may be proto stars) and are most likely members of the Cyg OB2 association. The large values of the flux ratio L sub IR/L sub VIS suggests that the central objects are obscured because of very large extinction.

  16. TCP over OBS - fixed-point load and loss.

    PubMed

    Cameron, Craig; Le Vu, Hai; Choi, Jung; Bilgrami, Syed; Zukerman, Moshe; Kang, Minho

    2005-11-14

    The sending rate of commonly used TCP protocols is tightly coupled to packet loss within the network: a high rate of packet loss will cause a sender to slow down, thereby reducing the network load and decreasing subsequent packet loss rates. In this paper, we combine a widely verified source rate TCP model with an Optical Burst Switching (OBS) loss model, to find fixed-point input loads and loss rates for an OBS link carrying TCP traffic. In doing so, we show that if OBS networks are to be efficiently used to carry TCP traffic, many wavelengths with full wavelength conversion are required.

  17. The interstellar medium in the ARA OB1 field

    NASA Astrophysics Data System (ADS)

    Rizzo, J. R.; Bajaja, E.

    1994-09-01

    We report here the results of a detailed study of two surveys of H I, at lambda = 21 cm, in the field of the stellar association Ara OB1. Subtracting the background with a procedure developed here, we were able to identify two H I shell-like features, at roughly the same position but at different velocities, whose parameters were determined. On the basis of their kinematical distances, two concentrations of OB stars (one at 1400 pc, belonging to Ara OB1, and another at 2500 pc) might be the progenitors of these shells. The nearest H I shell may have triggered a process of star formation.

  18. New Results on the Nearest OB Association: Sco-Cen (Sco OB2)

    NASA Astrophysics Data System (ADS)

    Mamajek, Eric E.

    2013-01-01

    The Scorpius-Centaurus OB association (Sco OB2) is the nearest site of recent massive star formation to the Sun. The primary stellar groups in the Sco-Cen complex (including OB subgroups Upper Sco, Upper Cen Lup, and Lower Cen Cru, the neighboring molecular cloud complexes Lup, Cha, CrA, Oph, and dispersed young groups Eta Cha, Epsilon Cha, TW Hya, and Beta Pic) have been participants in a complex episode of stellar birth (and some stellar death) over the past ~20 Myr. Here I summarize some recent results on the Sco-Cen complex from the U. Rochester group: (1) isochronal analysis of the HR diagram positions for >1 Msun stars in the Upper Scorpius subgroup shows it to be twice as old as previously thought (11 Myr vs. 5 Myr), (2) analysis of high resolution optical echelle spectra show that the subgroups are approximately solar in composition, (3) surveys for lower mass members are showing that the complex shows more substructure than previously recognized, including at least one new subgroup ("Lower Sco"), and the velocity and age data for the nearest OB subgroup Lower Cen Cru argue for a bifurcation into a younger 10 Myr) southern part ("Crux") and an older 20 Myr) northern part ("Lower Centaurus"), (4) an eclipsing, multi-ring dust disk system was serendipitously discovered in the SuperWASP and ASAS light curve for the newly discovered K5-type Sco-Cen member 1SWASP J140747.93-394542.6. With regard to some recent results by other investigators, we find that (1) attempts by some authors to subsume the Sco-Cen subgroups into a single sample of a single age are unnecessarily mixing samples with a wide range in ages, and (2) I have been unable to replicate the expansion age determinations claimed by some investigators for the TW Hya and Beta Pic groups (both purported to have expansion ages of 8 and 12 Myr, respectively), which have been used by some investigators to independently age-date the Sco-Cen subgroups. We acknowledge support from NSF grant AST-1008908 and the

  19. BOREAS TF-9 SSA-OBS Branch Level Flux Data

    NASA Technical Reports Server (NTRS)

    Rayment, Mark B.; Jarvis, Paul G.; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor)

    2000-01-01

    The BOREAS TF-9 team collected data that describe carbon dioxide and water vapor fluxes from foliage at the BOREAS SSA-OBS site from 07-April through 23-November-1996. The data are available in tabular ASCII files.

  20. obs4MIPS: Satellite Datasets for Model Evaluation

    NASA Astrophysics Data System (ADS)

    Ferraro, R.; Waliser, D. E.; Gleckler, P. J.

    2013-12-01

    This poster will review the current status of the obs4MIPs project, whose purpose is to provide a limited collection of well-established and documented datasets for comparison with Earth system models. These datasets have been reformatted to correspond with the CMIP5 model output requirements, and include technical documentation specifically targeted for their use in model output evaluation. There are currently over 50 datasets containing observations that directly correspond to CMIP5 model output variables. We will review the rational and requirements for obs4MIPs contributions, and provide summary information of the current obs4MIPs holdings on the Earth System Grid Federation. We will also provide some usage statistics, an update on governance for the obs4MIPs project, and plans for supporting CMIP6.

  1. Defective Phagocytosis of Apoptotic Cells by Macrophages in Atherosclerotic Lesions of ob/ob Mice and Reversal by a Fish Oil Diet

    PubMed Central

    Li, Suzhao; Sun, Yu; Liang, Chien-Ping; Thorp, Edward B.; Han, Seongah; Jehle, Andreas W.; Saraswathi, Viswanathan; Pridgen, Brian; Kanter, Jenny E.; Li, Rong; Welch, Carrie L.; Hasty, Alyssa H.; Bornfeldt, Karin E.; Breslow, Jan L.; Tabas, Ira; Tall, Alan R.

    2009-01-01

    Rationale The complications of atherosclerosis are a major cause of death and disability in Type 2 diabetes. Defective clearance of apoptotic cells by macrophages (efferocytosis) is thought to lead to increased necrotic core formation and inflammation in atherosclerotic lesions. Objective To determine if there is defective efferocytosis in a mouse model of obesity and atherosclerosis. Methods and Results We quantified efferocytosis in peritoneal macrophages and in atherosclerotic lesions of obese ob/ob or ob/ob;Ldlr−/− mice and littermate controls. Peritoneal macrophages from ob/ob and ob/ob;Ldlr−/− mice showed impaired efferocytosis, reflecting defective PI3K activation during uptake of apoptotic cells. Membrane lipid composition of ob/ob and ob/ob;Ldlr−/− macrophages showed an increased content of saturated fatty acids (FAs), and decreased n-3 FAs ((Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA)) compared to controls. A similar defect in efferocytosis was induced by treating control macrophages with saturated free FA (FFA)/BSA complexes, while the defect in ob/ob macrophages was reversed by treatment with EPA/BSA or by feeding ob/ob mice a fish oil diet rich in n-3 FAs. There was also defective macrophage efferocytosis in atherosclerotic lesions of ob/ob;Ldlr−/− mice and this was reversed by a fish oil-rich diet. Conclusions The findings suggest that in obesity and Type 2 diabetes elevated levels of saturated FAs and/or decreased levels of n-3 FAs contribute to decreased macrophage efferocytosis. Beneficial effects of fish oil diets in atherosclerotic cardiovascular disease may involve improvements in macrophage function related to reversal of defective efferocytosis, and could be particularly important in Type 2 diabetes and obesity. PMID:19834009

  2. Brown Adipose Tissue Transplantation Reverses Obesity in Ob/Ob Mice.

    PubMed

    Liu, Xiaomeng; Wang, Siping; You, Yilin; Meng, Minghui; Zheng, Zongji; Dong, Meng; Lin, Jun; Zhao, Qianwei; Zhang, Chuanhai; Yuan, Xiaoxue; Hu, Tao; Liu, Lieqin; Huang, Yuanyuan; Zhang, Lei; Wang, Dehua; Zhan, Jicheng; Jong Lee, Hyuek; Speakman, John R; Jin, Wanzhu

    2015-07-01

    Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased β3-adrenergic receptor and fatty acid oxidation related gene expression in subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT.

  3. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  4. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  5. OB associations in four stellar fields of M 31

    NASA Astrophysics Data System (ADS)

    Battinelli, P.

    1992-05-01

    The problem of the real spatial distribution of OB stars in M 31, with particular attention on whether these stars follow a hierarchical distribution in agreement with the scenario of star formation proposed by Elmegreen & Elmegreen (1983), is addressed. The first step in this direction would be to provide an objective identification of the OB associations in M 31 and then to study their spatial distribution. Unfortunately, this task is made difficult by the fact that the catalogs of OB stars in M 31 available in the literature are mainly limited to supergiant stars. The recent PLC identification technique for OB associations (Battinelli, 1991) is therefore applied in four stellar fields of M 31 for which high-resolution photometric data ensure that almost all of the OB stars have been identified. Results are in agreement with other works in the literature and show that the sizes of the OB associations in these fields of M 31 are similar to the sizes of their counterparts in the Milky Way and in the Magellanic Clouds.

  6. Multiplicity Survey of Bright Stars in the Nearby OB Associations

    NASA Astrophysics Data System (ADS)

    Balega, Yu. Yu.; Dyachenko, V. V.; Maksimov, A. F.; Malogolovets, E. V.; Rastegaev, D. A.

    2017-06-01

    We present the results of a speckle interferometric multiplicity survey of bright members of six OB associations: Per OB2, Per OB3 (α Per), CasTau, Lac OB1, Cep OB2, and Cep OB6.The observations were made with the diffraction spatial resolution of the 6-m BTA telescope in the period 2007-2016. To prepare the sample of 390 targets, we made use of the association member lists formed by de Zeeuw et al. (1999) using the Hipparcos astrometric data. The majority of the targets in the list (65%) fall into the category of intermediate-mass stars A5-B4, while only 77 objects (20%) refer to massive O and early B stars. We discovered 35 new binaries among the association members. Using the results of speckle interferometry, together with the literature and catalogue data on wide astrometric binaries, spectroscopic pairs and occultation binaries, we found that the average fraction of binaries and multiples (frequency of multiple systems) in the six associations is 0.28. The average number of components per star is 0.34. We established that the mass ratio distribution of the studied systems follows f(q)∝ qγ with γ = -0.7±0.2, in the range q = 0.3-1.0. Below q = 0.3, the difficulties of the detection of low-mass companions lead to uncertainties in the distribution.

  7. Quantifying the Components of the Field OB Star Population

    NASA Astrophysics Data System (ADS)

    Chen, Xinyi; Oey, M. S.; Lamb, Joel B.; Kushner, Cole

    2016-01-01

    Massive stars have long been thought to form in clusters. However, recent observations suggest some massive stars form in extremely sparse environments. Could these massive stars form alone? Clarifying the nature of field OB stars is central to this question. Defining these as OB stars that are isolated from other OB stars, the field comprises three distinct populations: "tip-of-the-iceberg stars", which are in small, but normal, clusters that only have one OB star; runaway stars; and any stars that form in situ in the field. The relative frequencies of each category are unknown. To study the population and obtain estimates of the frequencies, we examine 134 field OB stars from the Runaways and Isolated O-Type Star Spectroscopic Survey of the SMC (RIOTS4; Lamb et al. 2015) in the bar region of the Small Magellanic Cloud. These stars are at least 28 pc in projection away from other OB stars. We use OGLE-III (Udalski et al. 2008) I-band images to search for small, potential clusters around our target stars, using friends-of-friends and nearest-neighbor algorithms. We estimate the relative frequencies of tip-of-the-iceberg stars and isolated stars, some of which may have formed in situ. Thanks to Radek Poleski for help with the OGLE-III database. This work was supported by the NSF grants AST-0907758 and AST-1514838.

  8. Kefir improves fatty liver syndrome by inhibiting the lipogenesis pathway in leptin-deficient ob/ob knockout mice.

    PubMed

    Chen, H-L; Tung, Y-T; Tsai, C-L; Lai, C-W; Lai, Z-L; Tsai, H-C; Lin, Y-L; Wang, C-H; Chen, C-M

    2014-09-01

    Fatty liver disease is commonly associated with obesity, insulin resistance and diabetes. Severe fatty liver is sometimes accompanied by steatohepatitis and may lead to the development of hepatocellular carcinoma. At present, there is no effective treatment for non-alcoholic fatty liver disease (NAFLD); thus, recent investigations have focused on developing effective therapeutics to treat this condition. This study aimed to evaluate the effects of kefir on the hepatic lipid metabolism of ob/ob mice, which are commonly used to model fatty liver disease. In this study, we used leptin receptor-deficient ob/ob mice as an animal disease model of NAFLD. Six-week-old ob/ob mice were orally administered the dairy product kefir (140 mg kg(-1) of body weight (BW) per day) for 4 weeks. The data demonstrated that kefir improved fatty liver syndrome on BW, energy expenditure and basal metabolic rate by inhibiting serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities (P<0.05) and by decreasing the triglyceride (TG) and total cholesterol (TC) contents of the liver (P<0.05). Oral kefir administration also significantly reduced the macrovesicular fat quantity in liver tissue. In addition, kefir markedly decreased the expression of the genes sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) (P<0.05) but not the expression of peroxisome proliferator-activated receptor α (PPARα) or hepatic carnitine palmitoyltransferase-1α (CPT1α) in the livers of ob/ob mice. On the basis of these results, we conclude that kefir improves NAFLD on BW, energy expenditure and basal metabolic rate by inhibiting the lipogenesis pathway and that kefir may have the potential for clinical application to the prevention or treatment of NAFLD.

  9. Tumour necrosis factor-α inhibition with lenalidomide alleviates tissue oxidative injury and apoptosis in ob/ob obese mice.

    PubMed

    Zhu, Xiaoling; Jiang, Shasha; Hu, Nan; Luo, Fuling; Dong, Hailong; Kang, Yu-Ming; Jones, Kyla R; Zou, Yunzeng; Xiong, Lize; Ren, Jun

    2014-07-01

    Lenalidomide (Revlimid; Selleck Chemicals, Houston, TX, USA), an analogue of thalidomide, possesses potent cytokine modulatory capacity through inhibition of cytokines such as tumour necrosis factor (TNF)-α, a cytokine pivotal for the onset and development of complications in obesity and diabetes mellitus. The present study was designed to evaluate the effect of lenalidomide on oxidative stress, protein and DNA damage in multiple organs in an ob/ob murine model of obesity. To this end, C57BL/6 lean and ob/ob obese mice were administered lenalidomide (50 mg/kg per day, p.o.) for 5 days. Oxidative stress, protein and DNA damage were assessed using the conversion of reduced glutathione (GSH) to oxidized glutathione (GSSG), carbonyl formation and Comet assay, respectively. Apoptosis was evaluated using caspase 3 activity, and levels of Bax, Bcl-2, Bip, caspase 8, caspase 9 and TNF-α were assessed using western blot analysis. Lenalidomide treatment did not affect glucose clearance in lean or ob/ob mice. Obese mice exhibited a reduced GSH/GSSG ratio in the liver, gastrocnemius skeletal muscle and small intestine, as well as enhanced protein carbonyl formation, DNA damage and caspase 3 activity in the liver, kidney, skeletal muscle and intestine; these effects were alleviated by lenalidomide, with the exception of obesity-associated DNA damage in the liver and kidney. Western blot analysis revealed elevated TNF-α, Bax, Bcl-2, Bip, caspase 8 and caspase 9 in ob/ob mice with various degrees of reversal by lenalidomide treatment. Together, these data indicate that lenalidomide protects against obesity-induced tissue injury and protein damage, possibly in association with antagonism of cytokine production and cytokine-induced apoptosis and oxidative stress.

  10. Properties of M 31 OB associations.

    NASA Astrophysics Data System (ADS)

    Haiman, Z.; Magnier, E. A.; Battinelli, P.; Lewin, W. H. G.; van Paradijs, J.; Hasinger, G.; Pietsch, W.; Supper, R.; Truemper, J.

    1994-10-01

    Magnier et al. (1993) recently identified 174 OB associations in M 31 using the PLC method (Battinelli 1991) with the Magnier et al. (1992) catalogue. The objectivity of the PLC method combined with the depth and accuracy of the Magnier et al. BVRI CCD data make these identifications suitable for photometric studies. We have studied the following properties of the 11 richest associations, 9 groups of associations, the eastern and western arms of M 31, and the entire sample of associations: the extinction E_B-V_, the slope of the differential luminosity function (dLF) in B and V, and the slope of the initial mass function (IMF). The data are not of sufficient quality to determine the ages of associations more accurately than to show that they are younger than a few 10^7^ years old. The extinction ranges between 0.20 and 0.41 magnitudes (+/-0.05mag); the western arm is significantly more reddened than the eastern arm. We have also found evidence of a difference between the young stellar populations of the eastern and western arms. The difference is most pronounced in the slopes of the B band dLF of the two arms (east: 0.46+/-0.02, west: 0.79+/-0.04, {DELTA}: 0.33+/-0.045). There is also a marginal difference seen in both the slope of the V band dLF (east: 0.48+/-0.04, west: 0.64+/-0.05, {DELTA}: 0.16+/-0.064) and the slope of the IMF (east: -2.09+/-0.30, west: -3.02+/-0.33, {DELTA}: 0.93+/-0.45). The combined weight of these three pieces of evidence suggests a real difference between the stellar populations of the two arms. It is possible that the difference is not physical, but an observational effect due to, e.g., the different reddening, varying photometric scatter between the two arms or different resolution of the images.

  11. e-MERLIN 21 cm constraints on the mass-loss rates of OB stars in Cyg OB2

    NASA Astrophysics Data System (ADS)

    Morford, J. C.; Fenech, D. M.; Prinja, R. K.; Blomme, R.; Yates, J. A.

    2016-11-01

    We present e-MERLIN 21 cm (L-band) observations of single luminous OB stars in the Cygnus OB2 association, from the Cyg OB2 Radio Survey Legacy programme. The radio observations potentially offer the most straightforward, least model-dependent, determinations of mass-loss rates, and can be used to help resolve current discrepancies in mass-loss rates via clumped and structured hot star winds. We report here that the 21 cm flux densities of O3 to O6 supergiant and giant stars are less than ˜70 μJy. These fluxes may be translated to `smooth' wind mass-loss upper limits of ˜4.4-4.8 × 10-6 M⊙ yr -1 for O3 supergiants and ≲2.9 × 10-6 M⊙ yr -1 for B0 to B1 supergiants. The first ever resolved 21 cm detections of the hypergiant (and luminous blue variable candidate) Cyg OB2 #12 are discussed; for multiple observations separated by 14 d, we detect an ˜69 per cent increase in its flux density. Our constraints on the upper limits for the mass-loss rates of evolved OB stars in Cyg OB2 support the model that the inner wind region close to the stellar surface (where Hα forms) is more clumped than the very extended geometric region sampled by our radio observations.

  12. Gender-Specific Metabolomic Profiling of Obesity in Leptin-Deficient ob/ob Mice by 1H NMR Spectroscopy

    PubMed Central

    Kim, Sang-Woo; Jung, Youngae; Bae, Hyun-Whee; Lee, Daeyoup; Park, Sung Goo; Lee, Chul-Ho; Hwang, Geum-Sook; Chi, Seung-Wook

    2013-01-01

    Despite the numerous metabolic studies on obesity, gender bias in obesity has rarely been investigated. Here, we report the metabolomic analysis of obesity by using leptin-deficient ob/ob mice based on the gender. Metabolomic analyses of urine and serum from ob/ob mice compared with those from C57BL/6J lean mice, based on the 1H NMR spectroscopy in combination with multivariate statistical analysis, revealed clear metabolic differences between obese and lean mice. We also identified 48 urine and 22 serum metabolites that were statistically significantly altered in obese mice compared to lean controls. These metabolites are involved in amino acid metabolism (leucine, alanine, ariginine, lysine, and methionine), tricarbocylic acid cycle and glucose metabolism (pyruvate, citrate, glycolate, acetoacetate, and acetone), lipid metabolism (cholesterol and carnitine), creatine metabolism (creatine and creatinine), and gut-microbiome-derived metabolism (choline, TMAO, hippurate, p-cresol, isobutyrate, 2-hydroxyisobutyrate, methylamine, and trigonelline). Notably, our metabolomic studies showed distinct gender variations. The obese male mice metabolism was specifically associated with insulin signaling, whereas the obese female mice metabolism was associated with lipid metabolism. Taken together, our study identifies the biomarker signature for obesity in ob/ob mice and provides biochemical insights into the metabolic alteration in obesity based on gender. PMID:24098417

  13. Carnosine Attenuates the Development of both Type 2 Diabetes and Diabetic Nephropathy in BTBR ob/ob Mice

    PubMed Central

    Albrecht, Thomas; Schilperoort, Maaike; Zhang, Shiqi; Braun, Jana D.; Qiu, Jiedong; Rodriguez, Angelica; Pastene, Diego O.; Krämer, Bernhard K.; Köppel, Hannes; Baelde, Hans; de Heer, Emile; Anna Altomare, Alessandra; Regazzoni, Luca; Denisi, Alessandra; Aldini, Giancarlo; van den Born, Jacob; Yard, Benito A.; Hauske, Sibylle J.

    2017-01-01

    We previously demonstrated that polymorphisms in the carnosinase-1 gene (CNDP1) determine the risk of nephropathy in type 2 diabetic patients. Carnosine, the substrate of the enzyme encoded by this gene, is considered renoprotective and could possibly be used to treat diabetic nephropathy (DN). In this study, we examined the effect of carnosine treatment in vivo in BTBR (Black and Tan, BRachyuric) ob/ob mice, a type 2 diabetes model which develops a phenotype that closely resembles advanced human DN. Treatment of BTBR ob/ob mice with 4 mM carnosine for 18 weeks reduced plasma glucose and HbA1c, concomitant with elevated insulin and C-peptide levels. Also, albuminuria and kidney weights were reduced in carnosine-treated mice, which showed less glomerular hypertrophy due to a decrease in the surface area of Bowman’s capsule and space. Carnosine treatment restored the glomerular ultrastructure without affecting podocyte number, resulted in a modified molecular composition of the expanded mesangial matrix and led to the formation of carnosine-acrolein adducts. Our results demonstrate that treatment with carnosine improves glucose metabolism, albuminuria and pathology in BTBR ob/ob mice. Hence, carnosine could be a novel therapeutic strategy to treat patients with DN and/or be used to prevent DN in patients with diabetes. PMID:28281693

  14. Modulation of hepatic microsomal triglyceride transfer protein (MTP) induced by S-nitroso-N-acetylcysteine in ob/ob mice.

    PubMed

    Oliveira, Claudia P M S; Alves, Venâncio A F; Lima, Vicência M R; Stefano, José Tadeu; Debbas, Victor; Sá, Sandra Valéria; Wakamatsu, Alda; Corrêa-Giannella, Maria Lúcia; de Mello, Evandro Sobroza; Havaki, Sofia; Tiniakos, Dina G; Marinos, Evangelos; de Oliveira, Marcelo G; Giannella-Neto, Daniel; Laurindo, Francisco R; Caldwell, Stephen; Carrilho, Flair J

    2007-07-15

    We evaluated the effects of a potent NO donor, S-nitroso-N-acetylcysteine (SNAC), on microsomal triglyceride transfer protein (MTP) expression in ob/ob mice. NAFLD was induced in male ob/ob mice using a methionine-choline deficient diet (MCD) concomitantly with oral SNAC fed solution (n=5) or vehicle (control; n=5) by gavage daily for 4 weeks. Livers were collected for histology and for assessing MTP by RT-qPCR, Western blot, immunohistochemistry and immunogold electron microscopy analyses. Histological analysis showed diffuse macro and microvesicular steatosis, moderate hepatocellular ballooning and moderate inflammatory infiltrate in ob/ob mice fed the MCD diet. With SNAC, mice showed a marked reduction in liver steatosis (p<0.01), in parenchymal inflammation (p=0.02) and in MTP protein immunoexpression in zone III (p=0.05). Moreover, SNAC caused reduction of MTP protein in Western blot analysis (p<0.05). In contrast, MTP mRNA content was significantly higher (p<0.05) in mice receiving SNAC. Immuno-electron microscopy showed MTP localized in the rough endoplasmic reticulum of hepatocytes in both treated and untreated groups. However with SNAC treatment, MTP was also observed surrounding fat globules. Histological improvement mediated by a nitric oxide donor is associated with significantly altered expression and distribution of MTP in this animal model of fatty liver disease. Further studies are in progress to examine possible mechanisms and to develop SNAC as a possible therapy for human fatty liver disease.

  15. Reduced histone H3K9 acetylation of clock genes and abnormal glucose metabolism in ob/ob mice.

    PubMed

    Ishikawa-Kobayashi, Eiko; Ushijima, Kentarou; Ando, Hitoshi; Maekawa, Tomohiro; Takuma, Masashi; Furukawa, Yusuke; Fujimura, Akio

    2012-10-01

    Recent chronobiological studies found significant correlation between lack of clock function and metabolic abnormalities. We previously showed that clock gene expressions were dampened in the peripheral tissues of obese and diabetic ob/ob mice. However, the molecular mechanism of the disturbance remained to be determined. In this study, we demonstrated for the first time that acetylation levels of histone H3 lysine 9 (H3K9) at the promoter regions of clock genes, such as Dbp, Per2, and Bmal1, in the adipose tissue of ob/ob mice were significantly reduced compared with those of its control C57BL/6J mice. Treatment with histone deacetylase (HDAC) inhibitors increased Dbp, but not Per2 or Bmal1, mRNA expression in adipose tissue, and it decreased blood glucose in these animals. In addition, 2-deoxyglucose uptake activity was significantly suppressed by silencing Dbp expression in cultured adipocytes. These results suggest that reduced H3K9 acetylation and subsequent decreased mRNA expression of the Dbp gene in adipose tissue are involved in the mechanism of development of abnormal glucose metabolism in ob/ob mice.

  16. Stromgren and H beta photometry of OB associations and open clusters. I - ARA OB1

    NASA Astrophysics Data System (ADS)

    Kaltcheva, Nadia T.; Georgiev, Leonid N.

    1992-11-01

    Stromgren and H beta photometry of 38 stars in the field of Ara OB1 is presented. The excesses, the absolute magnitudes and the temperatures of the stars have been obtained. The masses and the ages are also estimated, based on the models of Maeder and Meynet. Two different groups have been distinguished by their space locations at V0 - MV = 10.23 and 12.01 mag (assuming a normal extinction law). The more distant group is younger and more massive. The magnitude and the excess of the WR star MR62 point to its probable membership of this group. There are good reasons to suspect a value of the total-to-selective absorption ratio that is greater than the normal 3.2, which leads to a significant reduction of the true distance moduli.

  17. Melatonin reduces obesity and restores adipokine patterns and metabolism in obese (ob/ob) mice.

    PubMed

    Favero, Gaia; Stacchiotti, Alessandra; Castrezzati, Stefania; Bonomini, Francesca; Albanese, Massimo; Rezzani, Rita; Rodella, Luigi Fabrizio

    2015-10-01

    The increasing incidence of obesity, leading to metabolic complications, is now recognized as a major public health problem. The adipocytes are not merely energy-storing cells, but they play crucial roles in the development of the so-called metabolic syndrome due to the adipocyte-derived bioactive factors such as adipokines, cytokines, and growth factors. The dysregulated production and secretion of adipokines seen in obesity is linked to the pathogenesis of the metabolic disease processes. In this study, we hypothesized that dietary melatonin administration would support an anti-inflammatory response and play an important role in energy metabolism in subcutaneous and visceral adipose tissues of obese mice and so may counteract some of the disruptive effects of obesity. Lean and obese mice (ob/ob) received melatonin or vehicle in drinking water for 8 weeks. Thereafter, they were evaluated for morphologic alteration, inflammatory cell infiltration, and the adipokine patterns in visceral and subcutaneous white fat depots. In obese mice treated with vehicle, we observed a significant increase in fat depots, inflammation, and a dysregulation of the adipokine network. In particular, we measured a significant reduction of adiponectin and an increase of tumor necrosis factor α, resistin, and visfatin in adipose tissue deposits. These changes were partially reversed when melatonin was supplemented to obese mice. Melatonin supplementation by regulating inflammatory infiltration ameliorates obesity-induced adipokine alteration, whereas melatonin administration in lean mice was unaffected. Thus, it is likely that melatonin would be provided in supplement form to control some of the disruptive effects on the basis of obesity pathogenic process.

  18. Hypothalamic Leptin Gene Therapy Reduces Bone Marrow Adiposity in ob/ob Mice Fed Regular and High-Fat Diets

    PubMed Central

    Lindenmaier, Laurence B.; Philbrick, Kenneth A.; Branscum, Adam J.; Kalra, Satya P.; Turner, Russell T.; Iwaniec, Urszula T.

    2016-01-01

    Low bone mass is often associated with elevated bone marrow adiposity. Since osteoblasts and adipocytes are derived from the same mesenchymal stem cell (MSC) progenitor, adipocyte formation may increase at the expense of osteoblast formation. Leptin is an adipocyte-derived hormone known to regulate energy and bone metabolism. Leptin deficiency and high-fat diet-induced obesity are associated with increased marrow adipose tissue (MAT) and reduced bone formation. Short-duration studies suggest that leptin treatment reduces MAT and increases bone formation in leptin-deficient ob/ob mice fed a regular diet. Here, we determined the long-duration impact of increased hypothalamic leptin on marrow adipocytes and osteoblasts in ob/ob mice following recombinant adeno-associated virus (rAAV) gene therapy. Eight- to 10-week-old male ob/ob mice were randomized into four groups: (1) untreated, (2) rAAV-Lep, (3) rAAV-green fluorescent protein (rAAV-GFP), or (4) pair-fed to rAAV-Lep. For vector administration, mice were injected intracerebroventricularly with either rAAV-leptin gene therapy (rAAV-Lep) or rAAV-GFP (9 × 107 particles) and maintained for 30 weeks. In a second study, the impact of increased hypothalamic leptin levels on MAT was determined in mice fed high-fat diets; ob/ob mice were randomized into two groups and treated with either rAAV-Lep or rAAV-GFP. At 7 weeks post-vector administration, half the mice in each group were switched to a high-fat diet for 8 weeks. Wild-type (WT) controls included age-matched mice fed regular or high-fat diet. High-fat diet resulted in a threefold increase in MAT in WT mice, whereas MAT was increased by leptin deficiency up to 50-fold. Hypothalamic leptin gene therapy increased osteoblast perimeter and osteoclast perimeter with minor change in cancellous bone architecture. The gene therapy decreased MAT levels in ob/ob mice fed regular or high-fat diet to values similar to WT mice fed regular diet. These findings suggest

  19. Consumption of a glucose diet enhances the sensitivity of pancreatic islets from adrenalectomized genetically obese (ob/ob) mice to glucose-induced insulin secretion.

    PubMed

    Mistry, A M; Chen, N G; Lee, Y S; Romsos, D R

    1995-03-01

    Consumption of a glucose diet for 4 d markedly elevates plasma insulin concentrations in adrenalectomized ob/ob mice. The present study examined regulation of insulin secretion from perifused pancreatic islets of female adrenalectomized genetically obese (ob/ob) and lean mice fed a glucose diet for 4 d. These mice were fed a high carbohydrate commercial diet for 21 d, or the high carbohydrate commercial diet for 17 d and a purified high glucose diet for the last 4 d of the 21-d feeding period. Adrenalectomy equalized plasma insulin concentrations, pancreatic islet size, rates of insulin secretion in response to 20 mmol/L glucose and insulin mRNA relative abundance in ob/ob and lean mice fed the commercial diet, but the threshold for glucose-induced insulin secretion determined by a linear glucose gradient remained lower in islets from adrenalectomized ob/ob mice than in those from lean mice (3.8 +/- 0.1 vs. 4.9 +/- 0.2 mmol/L glucose), and addition of acetylcholine to the perifusate lowered the threshold to only 2.0 +/- 0.1 mmol/L glucose in islets from ob/ob mice vs. 3.3 +/- 0.1 mmol/L glucose in lean mice. Switching from the commercial diet to the glucose diet for 4 d increased plasma insulin concentrations -10-fold in islets from adrenalectomized ob/ob mice without affecting islet size, 20 mmol/L glucose-induced insulin secretion or insulin mRNA abundance. Consumption of the glucose diet did, however, markedly lower the threshold for glucose-induced insulin secretion in islets from adrenalectomized ob/ob mice to approximate the abnormally low glucose thresholds in intact ob/ob mice.(ABSTRACT TRUNCATED AT 250 WORDS)

  20. Pentoxifylline aggravates fatty liver in obese and diabetic ob/ob mice by increasing intestinal glucose absorption and activating hepatic lipogenesis

    PubMed Central

    Massart, J; Robin, MA; Noury, F; Fautrel, A; Lettéron, P; Bado, A; Eliat, PA; Fromenty, B

    2012-01-01

    BACKGROUND AND PURPOSE Pentoxifylline is in clinical trials for non-alcoholic fatty liver disease and diabetic nephropathy. Metabolic and hepatic effects of pentoxifylline were assessed in a murine model of obesity and type 2 diabetes. EXPERIMENTAL APPROACH Pentoxifylline (100 mg·kg−1·day−1) was administered for 4 days or 3 weeks in lean and obese/diabetic ob/ob mice. Plasma lipids, glucose, other metabolites and relevant enzymes were measured by standard assays. Hepatic lipids in vivo were assessed with magnetic resonance spectroscopy and by histology. Hepatic extracts were also analysed with RT-PCR and Western blotting. KEY RESULTS Four days of pentoxifylline treatment slightly increased liver lipids in ob/ob mice. After 3 weeks, pentoxifylline exacerbated fatty liver and plasma transaminases in ob/ob mice but did not induce liver steatosis in lean mice. Plasma glucose was highest in fed, but not fasted, ob/ob mice treated with pentoxifylline. During the first 10 min of an oral glucose tolerance test, blood glucose increased more rapidly in pentoxifylline-treated mice. Jejunal expression of glucose transporter 2 isoform was increased in pentoxifylline-treated obese mice. Hepatic activity of carbohydrate response element binding protein (ChREBP) increased after pentoxifylline in ob/ob, but not lean, mice. Hepatic expression of lipogenic enzymes was highest in pentoxifylline-treated ob/ob mice. However, pentoxifylline reduced markers of oxidative stress and inflammation in ob/ob liver. CONCLUSION AND IMPLICATIONS Pentoxifylline exacerbated fatty liver in ob/ob mice through enhanced intestinal glucose absorption, increased postprandial glycaemia and activation of hepatic lipogenesis. Long-term treatment with pentoxifylline could worsen fatty liver in some patients with pre-existing hyperglycaemia. PMID:21740407

  1. No evidence of disk destruction by OB stars

    NASA Astrophysics Data System (ADS)

    Richert, Alexander J. W.; Feigelson, Eric

    2015-01-01

    It has been suggested that the hostile environments observed in massive star forming regions are inhospitable to protoplanetary disks and therefore to the formation of planets. The Orion Proplyds show disk evaporation by extreme ultraviolet (EUV) photons from Theta1 Orionis C (spectral type O6). In this work, we examine the spatial distributions of disk-bearing and non-disk bearing young stellar objects (YSOs) relative to OB stars in 17 massive star forming regions in the MYStIX (Massive Young Star-Forming Complex Study in Infrared and X-ray) survey. Any tendency of disky YSOs, identified by their infrared excess, to avoid OB stars would reveal complete disk destruction.We consider a sample of MYStIX that includes 78 O3-O9 stars, 256 B stars, 5,606 disky YSOs, and 5,794 non-disky YSOs. For each OB star, we compare the cumulative distribution functions of distances to disky and non-disky YSOs. We find no significant avoidance of OB stars by disky YSOs. This result indicates that OB stars are not sufficiently EUV-luminous and long-lived to completely destroy a disk within its ordinary lifetime. We therefore conclude that massive star forming regions are not clearly hostile to the formation of planets.

  2. Intra-islet lesions and lobular variations in β-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas

    PubMed Central

    Parween, Saba; Kostromina, Elena; Nord, Christoffer; Eriksson, Maria; Lindström, Per; Ahlgren, Ulf

    2016-01-01

    The leptin deficient ob/ob mouse is a widely used model for studies on initial aspects of metabolic disturbances leading to type 2 diabetes, including insulin resistance and obesity. Although it is generally accepted that ob/ob mice display a dramatic increase in β-cell mass to compensate for increased insulin demand, the spatial and quantitative dynamics of β-cell mass distribution in this model has not been assessed by modern optical 3D imaging techniques. We applied optical projection tomography and ultramicroscopy imaging to extract information about individual islet β-cell volumes throughout the volume of ob/ob pancreas between 4 and 52 weeks of age. Our data show that cystic lesions constitute a significant volume of the hyperplastic ob/ob islets. We propose that these lesions are formed by a mechanism involving extravasation of red blood cells/plasma due to increased islet vessel blood flow and vessel instability. Further, our data indicate that the primary lobular compartments of the ob/ob pancreas have different potentials for expanding their β-cell population. Unawareness of the characteristics of β-cell expansion in ob/ob mice presented in this report may significantly influence ex vivo and in vivo assessments of this model in studies of β-cell adaptation and function. PMID:27713548

  3. OB protein binds specifically to the choroid plexus of mice and rats.

    PubMed Central

    Devos, R; Richards, J G; Campfield, L A; Tartaglia, L A; Guisez, Y; van der Heyden, J; Travernier, J; Plaetinck, G; Burn, P

    1996-01-01

    Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8643634

  4. Substructure In The Scorpius-Centaurus OB Association

    NASA Astrophysics Data System (ADS)

    Pecaut, Mark J.; Mamajek, Eric E.

    2016-07-01

    OB associations are remnants of star formation on a large scale, producing everything from O- and B-type stars, down to the lowest mass brown dwarfs. OB associations represent the typical mode of star formation in the Galaxy. But is this process monolithic? We present the results of a survey for new, solar mass (0.7 - 1.3 Msun) members of Sco-Cen, the nearest OB Association to the Sun. We identify 150 new members and place the known B/A/F/G/K/M-type members on the Hertzsprung-Russell diagram. From these data, we construct an age map of Sco-Cen, occupying approximately 2,000 square degrees on the sky. These results indicate there is substantial substructure in Sco-Cen, and present the possibility that star formation on the largest scales can be considered a collection of many individual, small-scale star formation events along a giant molecular cloud.

  5. Study of the ice conditions in the Gulf of Ob

    NASA Astrophysics Data System (ADS)

    Zyryanov, D. V.; Smirnov, V. N.

    2006-03-01

    The results of theoretical studies of fracture lines in the ice floes of the Gulf of Ob as a function of the coefficient of internal sliding are presented in this paper. An estimate of the fractal dimensions of the emerging topological structures and the results of the calculation of stresses in the ice cover of the Gulf of Ob are presented as functions of different directions of external forcing caused by the joint action of winds and currents on the ice. The geographical locations of the zones of possible loads are shown, together with the estimates of the hummock sizes in the zones of the maximal compression of the ice floes in the Gulf of Ob.

  6. OB associations and giant molecular clouds in the galaxy

    NASA Technical Reports Server (NTRS)

    Williams, Jonathan; Mckee, Chris

    1993-01-01

    Giant molecular clouds (GMC's) are the sites of all OB star formation in the Galaxy. These OB stars typically form in large associations and photoionize the surrounding gas, eventually destroying the clouds from which they were born. CO surveys have revealed the distribution of GMC's in the Galaxy, and radio observations provide data on the distribution of associations. These results are extrapolated to determine Galactic mean distribution functions of each and then combined to determine how GMC's and OB associations are correlated. The resulting probability distribution of luminosity given cloud mass implies that although most of the molecular mass of the Galaxy is in massive star forming complexes, a large number of clouds above which massive star formation is extremely likely and abundant and below which it is almost certainly absent.

  7. Loss of FXR protects against diet-induced obesity and accelerates liver carcinogenesis in ob/ob mice.

    PubMed

    Zhang, Yanqiao; Ge, Xuemei; Heemstra, Lydia A; Chen, Wei-Dong; Xu, Jiesi; Smith, Joseph L; Ma, Huiyan; Kasim, Neda; Edwards, Peter A; Novak, Colleen M

    2012-02-01

    Farnesoid X receptor (FXR) is known to play important regulatory roles in bile acid, lipid, and carbohydrate metabolism. Aged (>12 months old) Fxr(-/-) mice also develop spontaneous liver carcinomas. In this report, we used three mouse models to investigate the role of FXR deficiency in obesity. As compared with low-density lipoprotein receptor (Ldlr) knockout (Ldlr(-/-)) mice, the Ldlr(-/-)Fxr(-/-) double-knockout mice were highly resistant to diet-induced obesity, which was associated with increased expression of genes involved in energy metabolism in the skeletal muscle and brown adipose tissue. Such a striking effect of FXR deficiency on obesity on an Ldlr(-/-) background led us to investigate whether FXR deficiency alone is sufficient to affect obesity. As compared with wild-type mice, Fxr(-/-) mice showed resistance to diet-induced weight gain. Interestingly, only female Fxr(-/-) mice showed significant resistance to diet-induced obesity, which was accompanied by increased energy expenditure in these mice. Finally, we determined the effect of FXR deficiency on obesity in a genetically obese and diabetic mouse model. We generated ob(-/-)Fxr(-/-) mice that were deficient in both Leptin and Fxr. On a chow diet, ob(-/-)Fxr(-/-) mice gained less body weight and had reduced body fat mass as compared with ob/ob mice. In addition, we observed liver carcinomas in 43% of young (<11 months old) Ob(-/-)Fxr(-/-) mice. Together these data indicate that loss of FXR prevents diet-induced or genetic obesity and accelerates liver carcinogenesis under diabetic conditions.

  8. OB association members in the ACT and TRC catalogues

    NASA Astrophysics Data System (ADS)

    Hoogerwerf, Ronnie

    2000-03-01

    The Hipparcos Catalogue contains members of nearby OB associations brighter than 12th magnitude in V. However, membership lists are complete only to magnitude V=7.3. In this paper we discuss whether proper motions listed in the `Astrographic Catalogue+Tycho' reference catalogue (ACT) and the Tycho Reference Catalogue (TRC), which are complete to V~10.5mag, can be used to find additional association members. Proper motions in the ACT/TRC have an average accuracy of ~3masyr-1. We search for ACT/TRC stars which have proper motions consistent with the spatial velocity of the Hipparcos members of the nearby OB associations already identified by de Zeeuw et al. These stars are first selected using a convergent-point method, and then subjected to further constraints on the proper-motion distribution, magnitude and colour to narrow down the final number of candidate members. Monte Carlo simulations show that the proper-motion distribution, magnitude, and colour constraints remove ~97per cent of the field stars, while at the same time retain more than 90per cent of the cluster stars. The procedure has been applied to five nearby associations: the three subgroups of Sco OB2, plus Per OB3 and Cep OB6. In all cases except Cep OB6, we find evidence for new association members fainter than the completeness limit of the Hipparcos Catalogue. However, narrow-band photometry and/or radial velocities are needed to pinpoint the cluster members, and to study their physical characteristics.

  9. Molecular outflows in the Monoceros OB1 molecular cloud

    NASA Technical Reports Server (NTRS)

    Margulis, Michael; Lada, Charles J.; Snell, Ronald L.

    1988-01-01

    Observations of J = 1-0 emission from CO in nine suspected molecular outflows in the Monoceros OB1 molecular cloud are presented. It is found that, if the five sources which are confirmed to be outflows conserve momentum as they evolve, they will sweep up at least 0.6 percent of the mass of the entire cloud before coming into pressure equilibrium with the ambient gas. This number indicates that it should take at most 160 episodes of similar outflow activity in order to sweep up the bulk of the Mon OB1 cloud to highly supersonic speeds.

  10. Radial system of dark globules in Ser OB2

    NASA Astrophysics Data System (ADS)

    Gyulbudaghian, A. L.

    2012-09-01

    A radial system illuminated by bright, central OB stars is discussed. 12CO(1-0) observations are made of three globules from this system. Three molecular clouds are detected which are dark globules of a radial system in Ser OB2. A group of IR stars near the boundary of a globule with a rim is studied. One of the IR stars with bright filaments is a YSO (young stellar object). The radial system contains two bright, Mshaped fronts located at the tip of a jet, which may have emerged from the Wolf-Rayet star WR113. Similar M-shaped fronts are also located near the object SNO 85.

  11. Marine TAIGER OBS Experiment and its future prospects

    NASA Astrophysics Data System (ADS)

    Lee, C.; Wang, T.; van Avendonk, H. J.; Huang, Y.; Lin, J.; Lallemand, S.; Klingelhoeher, F.

    2009-12-01

    A total of 260 OBSs were deployed in the marine TAIGER program from late March to late July, 2009. These data were collected by US Columbia University’s R/V Langseth as the big-power seismic shooting ship and 10 Taiwanese ships to take terms for supporting of the OBS experiment in the entire seismic cruises. The OBS were provided by the National Taiwan Ocean University, French IFREMER and Scripps Institution of Oceanography. During these 4 months, we have worked around Taiwan in the South China Sea, Luzon Arc, East Taiwan and West Philippine Basin. All efforts are put together by many earth scientists from Taiwan, USA and France under one major purpose, to get a better understanding of the Taiwan mountain building processes. As a result, these new data will provide as a base to combine with many other disciplinal studies, such as the multi-channel seismic, land recorded seismometer data, gravity and magnetic as well as the natural earthquake data recorded by the OBS during the experiment time. Four very preliminary OBS data analyses will be presented in the same T25 postal section. Beside the research, we also carried out our teaching to our students on board a Taiwanese student training ship, Yu-Yin No.2. Therefore, an educational post is also to be shown in the ED01 section. Even the data analyses are in an early stage, but we are exciting about it. For example, 3 OBS profiles (T4, T5 and T6) in the East Taiwan were shot twice in normal and reversed directions with different shot intervals (30 and 60 seconds per shot). This exercise will be important to interpret the complicate collision/subduction structures in the East Taiwan. Two OBS profiles (T1 and T2) in the Luzon Arc were shot 5 times in the separated R/V Langseth cruises (due to the typhoon effects), again with different shot intervals (20 and 60 seconds per shot). These will provide us more opportunities to examine the collisional features in between Taiwan and Luzon. One OBS long profile (550 km) was

  12. Reservation information sharing enhancement for deflection routing in OBS network.

    PubMed

    Gao, Donghui; Zhang, Hanyi; Zhou, Zhiyu

    2005-03-07

    The resource contention problem is critical in Just-Enough-Time (JET) based optical burst switching (OBS) networks. Although deflection routing (DR) reduces the contention probability in some degree, it does not give much improvement under heavy traffic load. This paper analyzed the inducement causing contention in OBS networks, and proposed Resource Information Sharing Enhancement (RISE) scheme. Theoretical analysis shows that this scheme achieves shorter length of the detour path than normal DR. We simulated this scheme on both full mesh network and practical 14-node NSFNET. The simulation results show that it gives at best 2 orders magnitude improvement in reducing the burst contention probability over its previous routing approaches.

  13. The SGLT2 inhibitor empagliflozin ameliorates early features of diabetic nephropathy in BTBR ob/ob type 2 diabetic mice with and without hypertension.

    PubMed

    Gembardt, Florian; Bartaun, Christoph; Jarzebska, Natalia; Mayoux, Eric; Todorov, Vladimir T; Hohenstein, Bernd; Hugo, Christian

    2014-08-01

    Diabetic nephropathy is the leading cause of end-stage renal disease in humans in the Western world. The recent development of Na+-glucose cotransporter 2 (SGLT2) inhibitors offers a new antidiabetic therapy via enhanced glucose excretion. Whether this strategy exerts beneficial effects on the development of type 2 diabetic nephropathy is still largely unclear. We investigated the effects of the specific SGLT2 inhibitor empagliflozin in BTBR.Cg-Lep<ob>/WiscJ (BTBR ob/ob) mice, which spontaneously develop type 2 diabetic nephropathy. In the first experiment, BTBR ob/ob mice received either a diet containing 300 ppm empagliflozin or equicaloric placebo chow for 12 wk. In the second experiment, BTBR ob/ob mice received 1 μg·kg body wt(-1)·day(-1) ANG II to induce arterial hypertension and were separated into the same two diet groups for 6 wk. In both experiments, empagliflozin treatment enhanced glucosuria, thereby lowering blood glucose. Independently of hypertension, empagliflozin reduced albuminuria in diabetic mice. However, empagliflozin treatment affected diabetes-related glomerular hypertrophy, markers of renal inflammation, and mesangial matrix expansion only in BTBR ob/ob mice without hypertension. In summary, empagliflozin demonstrated significant antihyperglycemic effects, differentially ameliorating early features of diabetic nephropathy in BTBR ob/ob mice with and without hypertension.

  14. Mining the Obscured OB Star Population in Carina

    NASA Astrophysics Data System (ADS)

    Smith, Michael

    2016-04-01

    Massive OB stars are very influential objects in the ecology of galaxies like our own. Current catalogues of Galactic OB stars are heavily biased towards bright (g < 13) objects, only typically including fainter objects when found in prominent star clusters (Garmany et al., 1982; Reed, 2003; Maíz-Apellaniz et al., 2004). Exploitation of the VST Photometric Hα Survey (VPHAS+) allows us to build a robust catalogue of photometrically-selected OB stars across the entire Southern Galactic plane, both within clusters and in the field, down to ∼20th magnitude in g. For the first time, a complete accounting of the OB star runaway phenomenon becomes possible. Along with making the primary selection using VPHAS+ colours, I have performed Markov-Chain Monte Carlo fitting of the spectral energy distributions of the selected stars by combining VPHAS+ u, g, r, i with published J, H, K photometry. This gives rough constraints on effective temperature and distance, whilst delivering much more precise reddening parameters A0 and RV - allowing us to build a much richer picture of how extinction and extinction laws vary across the Galactic Plane. My thesis begins with a description of the method of photometric selection of OB star candidates and its validation across a 2 square degree field including the well-known young massive star cluster Westerlund 2 (Mohr-Smith et al., 2015). Following on from this I present spectroscopy with AAOmega of 283 candidates identified by our method, which confirms that ∼94% of the sample are the expected O and early B stars. I then develop this method further and apply it to a Galactic Plane strip of 42 square-degrees that runs from the Carina Arm tangent region to the much studied massive cluster in NGC 3603. A new aspect I attend to in this expansion of method is tightening up the uniform photometric calibration of the data, paying particular attention to the always-challenging u band. This leads to a new and reliable catalogue of 5915 OB

  15. Hepatic VLDL production in ob/ob mice is not stimulated by massive de novo lipogenesis but is less sensitive to the suppressive effects of insulin.

    PubMed

    Wiegman, Coen H; Bandsma, Robert H J; Ouwens, Margriet; van der Sluijs, Fjodor H; Havinga, Rick; Boer, Theo; Reijngoud, Dirk-Jan; Romijn, Johannes A; Kuipers, Folkert

    2003-05-01

    Type 2 diabetes in humans is associated with increased de novo lipogenesis (DNL), increased fatty acid (FA) fluxes, decreased FA oxidation, and hepatic steatosis. In this condition, VLDL production is increased and resistant to suppressive effects of insulin. The relationships between hepatic FA metabolism, steatosis, and VLDL production are incompletely understood. We investigated VLDL-triglyceride and -apolipoprotein (apo)-B production in relation to DNL and insulin sensitivity in female ob/ob mice. Hepatic triglyceride (5-fold) and cholesteryl ester (15-fold) contents were increased in ob/ob mice compared with lean controls. Hepatic DNL was increased approximately 10-fold in ob/ob mice, whereas hepatic cholesterol synthesis was not affected. Basal rates of hepatic VLDL-triglyceride and -apoB100 production were similar between the groups. Hyperinsulinemic clamping reduced VLDL-triglyceride and -apoB100 production rates by approximately 60% and approximately 75%, respectively, in lean mice but only by approximately 20% and approximately 20%, respectively, in ob/ob mice. No differences in hepatic expression of genes encoding apoB and microsomal triglyceride transfer protein were found. Hepatic expression and protein phosphorylation of insulin receptor and insulin receptor substrate isoforms were reduced in ob/ob mice. Thus, strongly induced hepatic DNL is not associated with increased VLDL production in ob/ob mice, possibly related to differential hepatic zonation of apoB synthesis (periportal) and lipid accumulation (perivenous) and/or relatively low rates of cholesterogenesis. Insulin is unable to effectively suppress VLDL-triglyceride production in ob/ob mice, presumably because of impaired insulin signaling.

  16. OB-stars as extreme condition test beds

    NASA Astrophysics Data System (ADS)

    Puls, Joachim; Sundqvist, Jon O.; Rivero González, Jorge G.

    2011-07-01

    Massive stars are inherently extreme objects, in terms of radiation, mass loss, rotation, and sometimes also magnetic fields. Concentrating on a (personally biased) subset of processes related to pulsations, rapid rotation and its interplay with mass-loss, and the bi-stability mechanism, we will discuss how active (and normal) OB stars can serve as appropriate laboratories to provide further clues.

  17. Recent Seismic Experiments of OBS in the South China Sea

    NASA Astrophysics Data System (ADS)

    Ruan, A.; Li, J.; Wu, Z.

    2012-12-01

    Since 2006 some research institutions of China have carried out some important seismic experiments by using ocean bottom seismometer(OBS) in the South China Sea (SCS) and obtained many concrete progresses in modeling the crustal structure of SCS and also in understanding of its formation and evolution as well. In 2006 three wide-angle profiles were completed in the northern margin, named OBS2006-1 across the northwestern sub-basin, OBS2006-2 parallel to the sea basin boundary and OBS2006-3 across the Dongsha Rise and Chaoshan Depression respectively. In 2010 two wide- angle profiles were completed, named OBS2010-1 and OBS 2010-2 both perpendicular to the northern off-shore faulting system. During 2009-2011 four wide-angle profiles were completed in the southern margin, named OBS973-1 from southern margin to the southwestern sub-basin, OBS973-2 from Liyue Bank to the southwestern sub-basin and OBS973-3 from Xisha to the southwestern sub-basin, OBS2011-2 from Xisha to Hainan Island respectively. In 2011 two 3D seismic array of OBS were completed in the Zhongnan-Changlong sea mount chain and Huangyan-Zhenbei sea mount chain respectively. Here we present some primary but important results as follows. (1) The velocity model of OBS2006-1 indicates that the crust under the continental slope decreases from 21km to 11km, and to 7.7km in the northwestern sub-basin with Moho depth ascends from 21km to 11km. The tectonic geometry and velocity structure of the northwestern sub-basin and its margins on both sides shows symmetrical and conjugate and indicates pure shear mode of continental margin rifting mechanism. (2) The velocity model of OBS2006-3 reveals remarkable thickness with maximum 8 km of the Mesozoic sediment in Chaoshan Depression in which velocity increases downward from 4.4 km/s at top to 5.3 km/s at the bottom. The buried depth of Moho decreases from 24-25 km under Dongsha Rise to 17 km in the lower slope and an obviously velocity abnormal is detected in the upper

  18. Discussion - Winds and magnetic fields of active OB stars

    NASA Astrophysics Data System (ADS)

    Bouret, Jean-Claude; Cidale, Lydia

    2011-07-01

    The discussion on winds and magnetic fields of active OB stars was carried out by S. Owoki, G. Wade, M. Cantiello, O. Kochukhov, M. Smith, C. Neiner, T. Rivinius, H. Henrichs and R. Townsend. The topics were the ability to detect small and large scale magnetic fields in massive stars and the need to consider limits on photometric variability of the star surface brightness.

  19. Gender preferences in ObGyn clinic vs private patients.

    PubMed

    Johnson, Amy M; Schnatz, Peter F

    2009-04-01

    To determine whether clinic patients, compared to private practice patients, are more likely to prefer seeing a female physician. Connecticut women were surveyed from August through September 2001, inclusive, about the attributes they desire in an ObGyn provider. Clinic patient responses were analyzed in comparison to private patients. A total of 262 women completed the survey (32 clinic and 232 private practice patients). Demographics of the subgroups differed, as the clinic patients more likely to be younger, not married, and were primarily Hispanic. Clinic patients reported a decreased opportunity to choose the gender of their provider (33.4% vs 62.8%) and were more likely to prefer a female provider (79.3% vs 36.9%). Unlike the private patients, most clinic patients responded that female providers are more knowledgeable and understanding of women's health issues. Clinic patients have a stronger preference for female ObGyn's compared to private patients. Medical students who rotate through outpatient ObGyn clinic's are likely to have different patient encounter experiences than students who spend time in private ObGyn offices.

  20. OB/OOCH Upstream 2007: Reflections on an Upstream Journey

    ERIC Educational Resources Information Center

    Edmonstone, Laura

    2008-01-01

    In this article, the author shares her experience of spending time at Camp Oochigeas as an Outward Bound (OB) instructor. The author was there as part of a team, working in a program that partnered three strong organizations with a wealth of knowledge and experience in working with kids. Camp Oochigeas operates a volunteer-based program for kids…

  1. Traveltime tomographic inversion of obs data, offshore western Svalbard

    NASA Astrophysics Data System (ADS)

    Madrussani, G.; Rossi, G.; Camerlenghi, A.

    2003-04-01

    We present the results of the tomographic inversion of the data acquired within the HYDRATECH project offshore western Svalbard in the immediate vicinity of the Knipovich Ridge system. The data acquisition encompassed continuous single-channel high-resolution seismic reflection profiling, simultaneous multicomponent-OBS recording, echo-sounding. The acoustic source consisted of an array of two sleeve guns. The OBS instruments are from IFREMER, GEOMAR and Tromsø University: they were separated by 400 m, while the spacing between parallel seismic profiles was set to 200 m, crossing the OBS stations. The seismic lines, spanning a relevant azimuth interval, are typically about 10 km long, resulting in offsets of more than 5 km from the OBS instruments. The seismic profiles show a well-stratified sequence of reflections down to about 400-500 ms below the seabed, dipping southwestwards. The BSR is mostly evident on the downslope profiles at about 250 ms below sea-bed, being parallel to the sea floor reflection, with opposite polarity, and causing a change in amplitude and frequency content, and sometimes also in polarity. In order to define the velocity changes above and below the BSR, and therefore to identify the presence of gas hydrates and free gas below them, a total number of ten events were chosen (4 above the BSR and 6 below it). The picked horizons were selected among many others, based on the velocity profile obtained at the university of Birmingham through 2-D inversion of one OBS data (Westbrook et al., this session). To help the picking of the corresponding events on the OBS data, we built a preliminary model, based on the travel-times picked on the single channel data, converted to depth using initially the same one dimension velocity function and then the models obtained throughout the tomographic iterations. The travel-times so calculated and projected on the OBS gathers, allowed an easier identification of the events, and the use of a semi

  2. The massive star population of Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Drew, Janet E.; Mohr-Smith, Michael

    2015-05-01

    We have compiled a significantly updated and comprehensive census of massive stars in the nearby Cygnus OB2 association by gathering and homogenizing data from across the literature. The census contains 169 primary OB stars, including 52 O-type stars and 3 Wolf-Rayet stars. Spectral types and photometry are used to place the stars in a Hertzsprung-Russell diagram, which is compared to both non-rotating and rotating stellar evolution models, from which stellar masses and ages are calculated. The star formation history and mass function of the association are assessed, and both are found to be heavily influenced by the evolution of the most massive stars to their end states. We find that the mass function of the most massive stars is consistent with a `universal' power-law slope of Γ = 1.3. The age distribution inferred from stellar evolutionary models with rotation and the mass function suggest the majority of star formation occurred more or less continuously between 1 and 7 Myr ago, in agreement with studies of low- and intermediate-mass stars in the association. We identify a nearby young pulsar and runaway O-type star that may have originated in Cyg OB2 and suggest that the association has already seen its first supernova. Finally we use the census and mass function to calculate the total mass of the association of 16 500^{+3800}_{-2800} M⊙, at the low end, but consistent with, previous estimates of the total mass of Cyg OB2. Despite this Cyg OB2 is still one of the most massive groups of young stars known in our Galaxy making it a prime target for studies of star formation on the largest scales.

  3. ObsPy: A Python Toolbox for Seismology

    NASA Astrophysics Data System (ADS)

    Wassermann, J. M.; Krischer, L.; Megies, T.; Barsch, R.; Beyreuther, M.

    2013-12-01

    Python combines the power of a full-blown programming language with the flexibility and accessibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. ObsPy is a community-driven, open-source project extending Python's capabilities to fit the specific needs that arise when working with seismological data. It a) comes with a continuously growing signal processing toolbox that covers most tasks common in seismological analysis, b) provides read and write support for many common waveform, station and event metadata formats and c) enables access to various data centers, webservices and databases to retrieve waveform data and station/event metadata. In combination with mature and free Python packages like NumPy, SciPy, Matplotlib, IPython, Pandas, lxml, and PyQt, ObsPy makes it possible to develop complete workflows in Python, ranging from reading locally stored data or requesting data from one or more different data centers via signal analysis and data processing to visualization in GUI and web applications, output of modified/derived data and the creation of publication-quality figures. All functionality is extensively documented and the ObsPy Tutorial and Gallery give a good impression of the wide range of possible use cases. ObsPy is tested and running on Linux, OS X and Windows and comes with installation routines for these systems. ObsPy is developed in a test-driven approach and is available under the LGPLv3 open source licence. Users are welcome to request help, report bugs, propose enhancements or contribute code via either the user mailing list or the project page on GitHub.

  4. Normalization of adiponectin concentrations by leptin replacement in ob/ob mice is accompanied by reductions in systemic oxidative stress and inflammation.

    PubMed

    Frühbeck, Gema; Catalán, Victoria; Rodríguez, Amaia; Ramírez, Beatriz; Becerril, Sara; Portincasa, Piero; Gómez-Ambrosi, Javier

    2017-06-05

    The circulating concentrations of adiponectin, an antidiabetic adipokine, have been shown to be reduced in obesity, in relation to an increase in inflammation. The aim of the present work was to assess the effect of leptin replacement on adiponectin levels and expression as well as on markers of oxidative stress and inflammation in leptin-deficient ob/ob mice. Twelve-week-old male mice (n = 7-10 per group) were treated with either saline (wild type and ob/ob mice) or leptin (ob/ob mice) for 18 days. A third group of ob/ob mice was treated with saline and pair-fed to the amount of food consumed by the leptin-treated group. Leptin replacement restored values of adiponectin (P < 0.001), reduced circulating 8-isoprostane and serum amyloid A (SAA) levels (P < 0.05 for both), and significantly downregulated the increased gene expression of osteopontin (Spp1, P < 0.05), Saa3 (P < 0.05), Cd68 (P < 0.01), Il6 (P < 0.01) and NADPH oxidase (Nox1 and Nox2, P < 0.01) in the perirenal WAT and Spp1 (P < 0.05) in the liver of ob/ob mice. In cultured adipocytes from ob/ob mice, leptin increased (P < 0.05) the mRNA expression and secretion of adiponectin. We concluded that circulating concentrations of adiponectin are positively regulated by leptin and ameliorate obesity-associated oxidative stress and inflammation in mice.

  5. iPLA2β deficiency attenuates obesity and hepatic steatosis in ob/ob mice through hepatic fatty-acyl phospholipid remodeling.

    PubMed

    Deng, Xiuling; Wang, Jiliang; Jiao, Li; Utaipan, Tanyarath; Tuma-Kellner, Sabine; Schmitz, Gerd; Liebisch, Gerhard; Stremmel, Wolfgang; Chamulitrat, Walee

    2016-05-01

    PLA2G6 or GVIA calcium-independent PLA2 (iPLA2β) is identified as one of the NAFLD modifier genes in humans, and thought to be a target for NAFLD therapy. iPLA2β is known to play a house-keeping role in phospholipid metabolism and remodeling. However, its role in NAFLD pathogenesis has not been supported by results obtained from high-fat feeding of iPLA2β-null (PKO) mice. Unlike livers of human NAFLD and genetically obese rodents, fatty liver induced by high-fat diet is not associated with depletion of hepatic phospholipids. We therefore tested whether iPLA2β could regulate obesity and hepatic steatosis in leptin-deficient mice by cross-breeding PKO with ob/ob mice to generate ob/ob-PKO mice. Here we observed an improvement in ob/ob-PKO mice with significant reduction in serum enzymes, lipids, glucose, insulin as well as improved glucose tolerance, and reduction in islet hyperplasia. The improvement in hepatic steatosis measured by liver triglycerides, fatty acids and cholesterol esters was associated with decreased expression of PPARγ and de novo lipogenesis genes, and the reversal of β-oxidation gene expression. Notably, ob/ob livers contained depleted levels of lysophospholipids and phospholipids, and iPLA2β deficiency in ob/ob-PKO livers lowers the former, but replenished the latter particularly phosphatidylethanolamine (PE) and phosphatidylcholine (PC) that contained arachidonic (AA) and docosahexaenoic (DHA) acids. Compared with WT livers, PKO livers also contained increased PE and PC containing AA and DHA. Thus, iPLA2β deficiency protected against obesity and ob/ob fatty liver which was associated with hepatic fatty-acyl phospholipid remodeling. Our results support the deleterious role of iPLA2β in severe obesity associated NAFLD.

  6. Coupling parameters of the MIT OBS at two nearshore sites

    NASA Astrophysics Data System (ADS)

    Tréhu, Anne M.; Solomon, Sean C.

    1981-03-01

    A model representing the coupling of an ocean-bottom seismometer (OBS) to the seafloor as a mass-spring-dashpot system satisfactorily explains the results of transient tests performed on different instruments during the Lopez Island intercomparison test. In this paper, we compare the results obtained for the MIT OBS at Lopez Island to results from similar tests at a dockside site at Woods Hole, Massachusetts. The vertical instrument response at the Lopez Island site shows a highly damped resonance at a frequency of 22 Hz, whereas the response at the Woods Hole site shows a marked resonance at 13 Hz. The difference between the responses at the two sites can be qualitatively attributed to the difference between the surficial sediments.

  7. The B Fields in OB Stars (BOB) Survey

    NASA Astrophysics Data System (ADS)

    Morel, T.; Castro, N.; Fossati, L.; Hubrig, S.; Langer, N.; Przybilla, N.; Schöller, M.; Carroll, T.; Ilyin, I.; Irrgang, A.; Oskinova, L.; Schneider, F. R. N.; Díaz, S. Simon; Briquet, M.; González, J. F.; Kharchenko, N.; Nieva, M.-F.; Scholz, R.-D.; de Koter, A.; Hamann, W.-R.; Herrero, A.; Maíz Apellániz, J.; Sana, H.; Arlt, R.; Barbá, R.; Dufton, P.; Kholtygin, A.; Mathys, G.; Piskunov, A.; Reisenegger, A.; Spruit, H.; Yoon, S.-C.

    2015-01-01

    The B fields in OB stars (BOB) survey is an ESO large programme collecting spectropolarimetric observations for a large number of early-type stars in order to study the occurrence rate, properties, and ultimately the origin of magnetic fields in massive stars. As of July 2014, a total of 98 objects were observed over 20 nights with FORS2 and HARPSpol. Our preliminary results indicate that the fraction of magnetic OB stars with an organised, detectable field is low. This conclusion, now independently reached by two different surveys, has profound implications for any theoretical model attempting to explain the field formation in these objects. We discuss in this contribution some important issues addressed by our observations (e.g., the lower bound of the field strength) and the discovery of some remarkable objects.

  8. An infrared supershell surrounding the Cygnus OB1 association

    NASA Astrophysics Data System (ADS)

    Saken, Jon M.; Shull, J. M.; Garmany, Catharine D.; Nichols-Bohlin, Joy; Fesen, Robert A.

    1992-10-01

    New studies are reported of a large, 2 x 5 deg peanut-shaped cavity in the far-infrared emission seen using IRAS data for the Cygnus X region. A more complete and better defined infrared supershell than reported by Lozinskaya and Repin (1990) is found and connected to the Cyg OB1 association. It is shown that the cavity represents the early stages of a superbubble produced by the winds and possible SNe from 10 to 20 massive stars. The locations and properties of these stars are used to estimate the energy deposition rate and to understand the manner in which supershells form and propagate. In Cyg OB1, spatially distributed subclustering appears to have played an important role in determining the nonspherical morphology of the superbubble.

  9. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.

    PubMed Central

    Tsien, H C; Brusseau, G A; Hanson, R S; Waclett, L P

    1989-01-01

    The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 microM or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene. Images PMID:2515801

  10. An infrared supershell surrounding the Cygnus OB1 association

    NASA Technical Reports Server (NTRS)

    Saken, Jon M.; Shull, J. M.; Garmany, Catharine D.; Nichols-Bohlin, Joy; Fesen, Robert A.

    1992-01-01

    New studies are reported of a large, 2 x 5 deg peanut-shaped cavity in the far-infrared emission seen using IRAS data for the Cygnus X region. A more complete and better defined infrared supershell than reported by Lozinskaya and Repin (1990) is found and connected to the Cyg OB1 association. It is shown that the cavity represents the early stages of a superbubble produced by the winds and possible SNe from 10 to 20 massive stars. The locations and properties of these stars are used to estimate the energy deposition rate and to understand the manner in which supershells form and propagate. In Cyg OB1, spatially distributed subclustering appears to have played an important role in determining the nonspherical morphology of the superbubble.

  11. An infrared supershell surrounding the Cygnus OB1 association

    NASA Technical Reports Server (NTRS)

    Saken, Jon M.; Shull, J. M.; Garmany, Catharine D.; Nichols-Bohlin, Joy; Fesen, Robert A.

    1992-01-01

    New studies are reported of a large, 2 x 5 deg peanut-shaped cavity in the far-infrared emission seen using IRAS data for the Cygnus X region. A more complete and better defined infrared supershell than reported by Lozinskaya and Repin (1990) is found and connected to the Cyg OB1 association. It is shown that the cavity represents the early stages of a superbubble produced by the winds and possible SNe from 10 to 20 massive stars. The locations and properties of these stars are used to estimate the energy deposition rate and to understand the manner in which supershells form and propagate. In Cyg OB1, spatially distributed subclustering appears to have played an important role in determining the nonspherical morphology of the superbubble.

  12. Analysis of Hybrid Buffering and Retransmission in OBS Networks.

    PubMed

    Vanitha, D Veera; Sabrigiriraj, M

    2015-01-01

    Burst contention is a major problem in the Optical Burst Switching (OBS) networks. Due to inadequate contention resolution techniques, the burst loss is prominent in OBS. In order to resolve contention fiber delay lines, wavelength converters, deflection routing, burst segmentation, and retransmission are used. Each one has its own limitations. In this paper, a new hybrid scheme is proposed which combines buffering and retransmission, which increases the mean number of bursts processed in the system. In this hybrid method, retransmission with controllable arrival and uncontrollable arrival is analyzed. Normally all the bursts reach the first hop and few of them go for second hop to reach destination. After all the bursts reach the destination the server may go for maintenance activity or wait for the arrival of next burst. We model it as a batch arrival single server retrial queue with buffer. Numerical results are analyzed to show the mean number of bursts processed in the system with uncontrollable arrival and controllable arrivals.

  13. OB stars in the Leading Arm of the Magellanic Stream

    NASA Astrophysics Data System (ADS)

    Moni Bidin, C.; Casetti-Dinescu, D. I.; Méndez, R. A.; Girard, T. M.; Vieira, K.; Korchagin, V. I.; van Altena, W. F.

    2015-05-01

    We present our spectroscopic program aimed to study some new interesting features recently discovered in the Magellanic Cloud System. These were revealed by the spatial distribution of OB-type candidate stars selected based on UV, optical, and IR photometry and proper motions from existing large-area catalogs. As a pilot study of our project, we are studying OB-star candidates in the Leading Arm (LA) of the Magellanic Stream, a gaseous tidal structure with no stellar counterpart known so far. Our targets group in three clumps near regions of high HI density in the LA. If confirmed, these young stars would evidence recent star formation in the LA, and they would help better understand and constrain the formation of the LA and its interactions with the Milky Way.

  14. Converting near-bottom OBS measurements into suspended sediment concentrations

    USGS Publications Warehouse

    Xu, J. P.

    1997-01-01

    A method of estimating the mass proportion of nonhomogeneous sediment constituents in suspension is presented. This method provides a more accurate conversion of OBS measurements (volts) to suspended sediment concentrations (grams per liter). Given a set of calibration coefficients of end-members, the method allows one to obtain the spatially and temporally varying in situ coefficients that are used in the conversion. Field and/or laboratory experiments are needed to verify the method.

  15. FIVE MORE MASSIVE BINARIES IN THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Kiminki, Daniel C.; Kobulnicky, Henry A.; Gilbert, Ian; Bird, Sarah; Chunev, Georgi

    2009-06-15

    We present the orbital solutions for four OB spectroscopic binaries, MT145, GSC 03161 - 00815, 2MASS J20294666+4105083, and Schulte 73, and the partial orbital solution to the B spectroscopic binary, MT372, as part of an ongoing study to determine the distribution of orbital parameters for massive binaries in the Cygnus OB2 Association. MT145 is a new, single-lined, moderately eccentric (e = 0.291 {+-} 0.009) spectroscopic binary with period of 25.140 {+-} 0.008 days. GSC 03161 - 00815 is a slightly eccentric (e = 0.10 {+-} 0.01), eclipsing, interacting and double-lined spectroscopic binary with a period of 4.674 {+-} 0.004 days. 2MASS J20294666+4105083 is a moderately eccentric (e = 0.273 {+-} 0.002) double-lined spectroscopic binary with a period of 2.884 {+-} 0.001 days. Schulte 73 is a slightly eccentric (e = 0.169 {+-} 0.009), double-lined spectroscopic binary with a period of 17.28 {+-} 0.03 days and the first 'twin' in our survey with a mass ratio of q = 0.99 {+-} 0.02. MT372 is a single-lined, eclipsing system with a period of 2.228 days and low eccentricity (e {approx} 0). Of the now 18 known OB binaries in Cyg OB2, 14 have periods and mass ratios. Emerging evidence also shows that the distribution of log(P) is flat and consistent with 'Oepik's Law'.

  16. A spectroscopic and photometric survey of OB+ stars

    NASA Technical Reports Server (NTRS)

    Drilling, J. S.

    1987-01-01

    Image-tube spectra (with dispersions of 40-90 A/mm) and UBV photometry of OB+ stars have been obtained at Cerro Tololo and Kitt Peak. The survey is complete to galactic latitudes of + or - 30 deg for galactic longitudes of between 300 and 60 deg. Most of the stars are found to be O-type and B-type supergiants, and 378 new O-type and B-type detections have been identified.

  17. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  18. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  19. PHOTOEVAPORATING PROPLYD-LIKE OBJECTS IN CYGNUS OB2

    SciTech Connect

    Wright, Nicholas J.; Drake, Jeremy J.; Guarcello, Mario G.; Hora, Joseph L.; Drew, Janet E.; Gutermuth, Robert A.; Kraemer, Kathleen E.

    2012-02-20

    We report the discovery of 10 proplyd-like objects in the vicinity of the massive OB association Cygnus OB2. They were discovered in IPHAS H{alpha} images and are clearly resolved in broadband Hubble Space Telescope/Advanced Camera for Surveys, near-IR, and Spitzer mid-IR images. All exhibit the familiar tadpole shape seen in photoevaporating objects such as the Orion proplyds, with a bright ionization front at the head facing the central cluster of massive stars and a tail stretching in the opposite direction. Many also show secondary ionization fronts, complex tail morphologies, or multiple heads. We consider the evidence that these are either proplyds or 'evaporating gaseous globules' (EGGs) left over from a fragmenting molecular cloud, but find that neither scenario fully explains the observations. Typical sizes are 50,000-100,000 AU, larger than the Orion proplyds, but in agreement with the theoretical scaling of proplyd size with distance from the ionizing source. These objects are located at projected separations of {approx}6-14 pc from the OB association, compared to {approx}0.1 pc for the Orion proplyds, but are clearly being photoionized by the {approx}65 O-type stars in Cyg OB2. Central star candidates are identified in near- and mid-IR images, supporting the proplyd scenario, though their large sizes and notable asymmetries are more consistent with the EGG scenario. A third possibility is therefore considered that these are a unique class of photoevaporating partially embedded young stellar objects that have survived the destruction of their natal molecular cloud. This has implications for the properties of stars that form in the vicinity of massive stars.

  20. A Preliminary OBS Data Analysis in the Luzon Arc

    NASA Astrophysics Data System (ADS)

    Wu, H.; Chen, H.; Liang, C.; Lee, C.; Wang, T.

    2009-12-01

    During Leg 1 and 4 of the TAIGER experiment (April-July, 2009), 2 OBS profiles (T2 and T1) were shot by R/V Langseth in the Luzon Arc. Because of the influence by the typhoon and the OBS ship arrangement, these 2 lines were shot 5 times. The T2 lines consists of the MGL0908_01 and MGL0908_09. In addition, a line with different angle, the MGL0905_27, was shot during Leg 1. The T1 line includes the MGL0905_25, MGL0908_05 and MGL0908_07. Along the MGL0908_01 line, we have used 12 American OBSs, the rest 4 sections (a total of 55 OBSs) are the Taiwanese OBSs. A preliminary analysis of the Taiwanese 4 sections, we can demonstrate the major features such as the Manila subduction zone, Luzon arc and part of the West Philippine Basin. We need to combine with the American section in order to improve the understanding of the deep structure in the Luzon Arc. Drs. Char-Shine Liu, Francis Wu, Shu-Kun Hsu and Kirk MacIntash were the chief scientists of Leg 1 and 4 of TAIGER cruise on board R/V Langseth. We provided several ships to support the OBS experiment. These data were collected by a multi-ship operation.

  1. Development of an anorthositic lunar regolith simulant: OB-1

    NASA Astrophysics Data System (ADS)

    Battler, Melissa Marie

    With the expected return of astronauts to the Moon before 2020 and construction of a permanent lunar base, there is a renewed need to understand the lunar surface environment. It is necessary to determine optimal methods for operations in the lunar regolith at selected landing sites. Regolith is the unconsolidated, abrasive material blanketing the lunar surface (up to ˜15 m thick), and will be the point of contact for humans and infrastructure. It is unlike any material naturally occurring on Earth. This thesis describes the development of the anorthositic physical/mechanical lunar regolith simulant "OB-I", designed for testing robotic lunar drilling and excavation equipment. The simulant comprises terrestrial anorthosite and slag, which have been comminuted and sized with jaw, roll, and ring crushers. OB-1 has been characterized using optical and electron microscopy, X-ray fluorescence spectroscopy, and various particle sizing methods. The resultant grain shapes, size distributions, and mineralogies are comparable to Apollo 16 regolith samples, making the OB-1 simulant appropriate for validating robotic lunar equipment on Earth.

  2. ObsPy: A Python Toolbox for Seismology

    NASA Astrophysics Data System (ADS)

    Krischer, Lion; Megies, Tobias; Sales de Andrade, Elliott; Barsch, Robert; MacCarthy, Jonathan

    2017-04-01

    In recent years the Python ecosystem evolved into one of the most powerful and productive scientific environments across disciplines. ObsPy (https://www.obspy.org) is a fully community-driven, open-source project dedicated to providing a bridge for seismology into that ecosystem. It does so by offering Read and write support for essentially every commonly used data format in seismology with a unified interface and automatic format detection. This includes waveform data (MiniSEED, SAC, SEG-Y, Reftek, …) as well as station (SEED, StationXML, …) and event meta information (QuakeML, ZMAP, …). Integrated access to the largest data centers, web services, and real-time data streams (FDSNWS, ArcLink, SeedLink, ...). A powerful signal processing toolbox tuned to the specific needs of seismologists. Utility functionality like travel time calculations with the TauP method, geodetic functions, and data visualizations. ObsPy has been in constant development for more than seven years and is developed and used by scientists around the world with successful applications in all branches of seismology. Additionally it nowadays serves as the foundation for a large number of more specialized packages. This presentation will give a short overview of the capabilities of ObsPy and point out several representative or new use cases. Additionally we will discuss the road ahead as well as the long-term sustainability of open-source scientific software.

  3. Non-thermal emission in Cyg OB2

    NASA Astrophysics Data System (ADS)

    Fenech, D.; Morford, J.; Prinja, R.

    2017-03-01

    We report here on the first results from the Cyg OB2 Radio Survey (COBRaS), which is a UCL-led e-MERLIN legacy project to provide a deep-field radio mapping of the Cygnus OB2 association. The project has been awarded a total allocation of 252 hours at C-band (5 GHz) and 42 hours at L-band (1.6 GHz) to image the core of the cluster. We discuss in particular the presence of non-thermal radio emission at 20 cm (L-band), and its potential as a highly efficient way to identify binaries via single-epoch observations, particularly for colliding-wind binaries. COBRaS data will provide a powerful tool for establishing binary incidence in Cyg OB2, specifically in the difficult intermediate-period range (1-100 yr). Ultimately, we aim to assemble a substantial and uniquely sensitive radio dataset, which will be exploited to address several fundamentally important areas of stellar astrophysics, including mass-loss, binary frequency, stellar cluster dynamics, and triggered star-formation.

  4. ADDITIONAL MASSIVE BINARIES IN THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Kiminki, Daniel C.; Kobulnicky, Henry A.; Ewing, Ian; Lundquist, Michael; Alexander, Michael; Vargas-Alvarez, Carlos; Choi, Heather; Bagley Kiminki, Megan M.; Henderson, C. B.

    2012-03-01

    We report the discovery and orbital solutions for two new OB binaries in the Cygnus OB2 Association, MT311 (B2V + B3V) and MT605 (B0.5V + B2.5:V). We also identify the system MT429 as a probable triple system consisting of a tight eclipsing 2.97 day B3V+B6V pair and a B0V at a projected separation of 138 AU. We further provide the first spectroscopic orbital solutions to the eclipsing, double-lined, O-star binary MT696 (O9.5V + B1:V), the double-lined, early B binary MT720 (B0-1V + B1-2V), and the double-lined, O-star binary MT771 (O7V + O9V). These systems exhibit orbital periods between 1.5 days and 12.3 days, with the majority having P <6 days. The two new binary discoveries and six spectroscopic solutions bring the total number of known massive binaries in the central region of the Cygnus OB2 Association to 20, with all but two having full orbital solutions.

  5. A SAMPLE OF OB STARS THAT FORMED IN THE FIELD

    SciTech Connect

    Oey, M. S.; Lamb, J. B.; Kushner, C. T.; Pellegrini, E. W.; Graus, A. S.

    2013-05-01

    We present a sample of 14 OB stars in the Small Magellanic Cloud that meet strong criteria for having formed under extremely sparse star-forming conditions in the field. These stars are a minimum of 28 pc in projection from other OB stars, and they are centered within symmetric, round H II regions. They show no evidence of bow shocks, implying that the targets are not transverse runaway stars. Their radial velocities relative to local H I also indicate that they are not line-of-sight runaway stars. A friends-of-friends analysis shows that nine of the objects present a few low-mass companion stars, with typical mass ratios for the two highest-mass stars of around 0.1. This further substantiates that these OB stars formed in place, and that they can and do form in extremely sparse conditions. This poses strong constraints on theories of star formation and challenges proposed relations between cluster mass and maximum stellar mass.

  6. Leptin Reduces the Expression and Increases the Phosphorylation of the Negative Regulators of GLUT4 Traffic TBC1D1 and TBC1D4 in Muscle of ob/ob Mice

    PubMed Central

    Sáinz, Neira; Rodríguez, Amaia; Catalán, Victoria; Becerril, Sara; Ramírez, Beatriz; Lancha, Andoni; Burgos-Ramos, Emma; Gómez-Ambrosi, Javier; Frühbeck, Gema

    2012-01-01

    Leptin improves insulin sensitivity in skeletal muscle. Our goal was to determine whether proteins controlling GLUT4 traffic are altered by leptin deficiency and in vivo leptin administration in skeletal muscle of wild type and ob/ob mice. Leptin-deficient ob/ob mice were divided in three groups: control, leptin-treated (1 mg/kg/d) and leptin pair-fed ob/ob mice. Microarray analysis revealed that 1,546 and 1,127 genes were regulated by leptin deficiency and leptin treatment, respectively. Among these, we identified 24 genes involved in intracellular vesicle-mediated transport in ob/ob mice. TBC1 domain family, member 1 (Tbc1d1), a negative regulator of GLUT4 translocation, was up-regulated (P = 0.001) in ob/ob mice as compared to wild types. Importantly, leptin treatment reduced the transcript levels of Tbc1d1 (P<0.001) and Tbc1d4 (P = 0.004) in the leptin-treated ob/ob as compared to pair-fed ob/ob animals. In addition, phosphorylation levels of TBC1D1 and TBC1D4 were enhanced in leptin-treated ob/ob as compared to control ob/ob (P = 0.015 and P = 0.023, respectively) and pair-fed ob/ob (P = 0.036 and P = 0.034, respectively) mice. Despite similar GLUT4 protein expression in wild type and ob/ob groups a different immunolocalization of this protein was evidenced in muscle sections. Leptin treatment increased GLUT4 immunoreactivity in gastrocnemius and extensor digitorum longus sections of leptin-treated ob/ob mice. Moreover, GLUT4 protein detected in immunoprecipitates from TBC1D4 was reduced by leptin replacement compared to control ob/ob (P = 0.013) and pair-fed ob/ob (P = 0.037) mice. Our findings suggest that leptin enhances the intracellular GLUT4 transport in skeletal muscle of ob/ob animals by reducing the expression and activity of the negative regulators of GLUT4 traffic TBC1D1 and TBC1D4. PMID:22253718

  7. Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor gamma.

    PubMed Central

    De Vos, P; Lefebvre, A M; Miller, S G; Guerre-Millo, M; Wong, K; Saladin, R; Hamann, L G; Staels, B; Briggs, M R; Auwerx, J

    1996-01-01

    The ob gene product, leptin, is a signaling factor regulating body weight and energy balance. ob gene expression in rodents is increased in obesity and is regulated by feeding patterns and hormones, such as insulin and glucocorticoids. In humans with gross obesity, ob mRNA levels are higher, but other modulators of human ob expression are unknown. In view of the importance of peroxisome proliferator-activated receptor gamma (PPARgamma) in adipocyte differentiation, we analyzed whether ob gene expression is subject to regulation by factors activating PPARs. Treatment of rats with the PPARalpha activator fenofibrate did not change adipose tissue and body weight and had no significant effect on ob mRNA levels. However, administration of the thiazolidinedione BRL49653, a PPARgamma ligand, increased food intake and adipose tissue weight while reducing ob mRNA levels in rats in a dose-dependent manner. The inhibitory action of the thiazolidinedione BRL49653 on ob mRNA levels was also observed in vitro. Thiazolidinediones reduced the expression of the human ob promoter in primary adipocytes, however, in undifferentiated 3T3-L1 preadipocytes lacking endogenous PPARgamma, cotransfection of PPARgamma was required to observe the decrease. In conclusion, these data suggest that PPARgamma activators reduce ob mRNA levels through an effect of PPARgamma on the ob promoter. PMID:8770873

  8. Insulin sensitivity improvement of fermented Korean Red Ginseng (Panax ginseng) mediated by insulin resistance hallmarks in old-aged ob/ob mice

    PubMed Central

    Cheon, Jeong-Mu; Kim, Dae-Ik; Kim, Kil-Soo

    2015-01-01

    Background The biological actions of various ginseng extracts have been studied for treating obesity and diabetes mellitus. However, few studies have evaluated the effects of fermented Korean Red Ginseng (Panax ginseng Meyer) on metabolic syndrome. The present study evaluated the antiobesity and antidiabetic effects of fermented red ginseng (FRG) on old-aged, obese, leptin-deficient (B6.V-Lepob, “ob/ob”) mice. Methods The animals were divided into three groups and given water containing 0%, 0.5%, and 1.0% FRG for 16 wk. The effect of FRG on ob/ob mice was determined by measuring changes in body weight, levels of blood glucose, serum contents of triglycerides, total cholesterol and free fatty acids, messenger RNA (mRNA) expressions of key factors associated with insulin action, such as insulin receptor (IR), lipoprotein lipase (LPL), glucose transporter 1 and 4 (GLUT1 and GLUT4), peroxisome proliferators-activated receptor gamma (PPAR-γ), and phosphoenolpyruvate carboxykinase (PEPCK) in the liver and in muscle, and histology of the liver and pancreas. Results FRG-treated mice had decreased body weight and blood glucose levels compared with control ob/ob mice. However, anti-obesity effect of FRG was not evident rather than hypoglycemic effect in old aged ob/ob mice. The hyperlipidemia in control group was attenuated in FRG-treated ob/ob mice. The mRNA expressions of IR, LPL, GLUT1, GLUT4, PPAR-γ, and PEPCK in the liver and in muscle were increased in the FRG-treated groups compared with the control group. Conclusion These results suggest that FRG may play a vital role in improving insulin sensitivity relative to reducing body weight in old-aged ob/ob mice. PMID:26869825

  9. Anorectic, thermogenic and anti-obesity activity of a selective orexin-1 receptor antagonist in ob/ob mice.

    PubMed

    Haynes, Andrea C; Chapman, Helen; Taylor, Colleen; Moore, Gary B T; Cawthorne, Michael A; Tadayyon, Mohammad; Clapham, John C; Arch, Jonathan R S

    2002-03-15

    A single dose of the orexin-1 (OX1) receptor antagonist 1-(2-methylbenzoxazol-6-yl)-3-[1,5] naphthyridin-4-yl urea hydrochloride (SB-334867-A) reduces orexin-A-induced feeding and natural feeding in Sprague Dawley rats. In this study, the anti-obesity effects of SB-334867-A were determined in genetically obese (ob/ob) mice dosed with SB-334867-A (30 mg/kg, i.p.) once daily for 7 days, and then twice daily for a further 7 days. SB-334867-A reduced cumulative food intake and body weight gain over 14 days. Total fat mass gain, determined by Dual Emission X-ray Absorptiometry, was reduced, while gain in fat-free mass was unchanged. Fasting (5 h) blood glucose was also reduced at the end of the study, with a trend to reduced plasma insulin. Interscapular brown adipose tissue (BAT) weight was reduced, the tissue was noticeably darker in colour and quantitative PCR (TaqMan) analysis of this tissue showed a trend to an increase in uncoupling protein-1 mRNA expression, suggesting that SB-334867-A might stimulate thermogenesis. This was confirmed in a separate study in which a single dose of SB-334867-A (30 mg/kg, i.p.) increased metabolic rate over 4 h in ob/ob mice. OX1 receptor mRNA was detected in BAT, and its expression was increased by 58% by treatment with SB-334867-A. This is the first demonstration that OX1 receptor antagonists have potential as both anti-obesity and anti-diabetic agents.

  10. Cygnus OB2 DANCe: A high-precision proper motion study of the Cygnus OB2 association

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Bouy, Herve; Drew, Janet E.; Sarro, Luis Manuel; Bertin, Emmanuel; Cuillandre, Jean-Charles; Barrado, David

    2016-08-01

    We present a high-precision proper motion study of 873 X-ray and spectroscopically selected stars in the massive OB association Cygnus OB2 as part of the DANCe project. These were calculated from images spanning a 15 yr baseline and have typical precisions <1 mas yr-1. We calculate the velocity dispersion in the two axes to be σ _α (c) = 13.0^{+0.8}_{-0.7} and σ _δ (c) = 9.1^{+0.5}_{-0.5} km s-1, using a two-component, two-dimensional model that takes into account the uncertainties on the measurements. This gives a three-dimensional velocity dispersion of σ3D = 17.8 ± 0.6 km s-1 implying a virial mass significantly larger than the observed stellar mass, confirming that the association is gravitationally unbound. The association appears to be dynamically unevolved, as evidenced by considerable kinematic substructure, non-isotropic velocity dispersions and a lack of energy equipartition. The proper motions show no evidence for a global expansion pattern, with approximately the same amount of kinetic energy in expansion as there is in contraction, which argues against the association being an expanded star cluster disrupted by process such as residual gas expulsion or tidal heating. The kinematic substructures, which appear to be close to virial equilibrium and have typical masses of 40-400 M⊙, also do not appear to have been affected by the expulsion of the residual gas. We conclude that Cyg OB2 was most likely born highly substructured and globally unbound, with the individual subgroups born in (or close to) virial equilibrium, and that the OB association has not experienced significant dynamical evolution since then.

  11. Leptin normalizes photic synchronization in male ob/ob mice, via indirect effects on the suprachiasmatic nucleus.

    PubMed

    Grosbellet, Edith; Gourmelen, Sylviane; Pévet, Paul; Criscuolo, François; Challet, Etienne

    2015-03-01

    Mounting evidence indicates a strong link between metabolic diseases and circadian dysfunctions. The metabolic hormone leptin, substantially increased in dietary obesity, displays chronobiotic properties. Here we investigated whether leptin is involved in the alteration of timing associated with obesity, via direct or indirect effects on the suprachiasmatic nucleus (SCN), the site of the master clock. Photic synchronization was studied in obese ob/ob mice (deficient in leptin), either injected or not with high doses of recombinant murine leptin (5 mg/kg). This was performed first at a behavioral level, by shifting the light-dark cycle and inducing phase shifts by 30-minute light pulses and then at molecular levels (c-FOS and P-ERK1/2). Moreover, to characterize the targets mediating the chronomodulatory effects of leptin, we studied the induction of phosphorylated signal transducer and activator of transcription 3 (P-STAT3) in the SCN and in different structures projecting to the SCN, including the medial hypothalamus. Ob/ob mice showed altered photic synchronization, including augmented light-induced phase delays. Acute leptin treatment normalized the photic responses of the SCN at both the behavioral and molecular levels (decrease of light-induced c-FOS). Leptin-induced P-STAT3 was modulated by light in the arcuate nucleus and both the ventromedial and dorsomedial hypothalamic nuclei, whereas its expression was independent of the presence of leptin in the SCN. These results suggest an indirect action of leptin on the SCN, possibly mediated by the medial hypothalamus. Taken together, these results highlight a central role of leptin in the relationship between metabolic disturbances and circadian disruptions.

  12. Close binary systems in OB-associations regions. V. the star V456 Cyg in direction of Cyg OB1

    NASA Astrophysics Data System (ADS)

    Zakirov, M. M.; Eshankulova, M. U.

    2006-10-01

    New UBVR photoelectric observations of the eclipsing variable star V456 Cyg of Algol type were obtained at the Maidanak Observatory in 1999-2003. The new ephemeris of the binary is: Min I=JDH2444911.3592(7) + {0.89119484 (14)d}\\cdot{E} + {0.15(1)}d\\cdot 10-9{\\cdot E2}. All of the light curves of the star V456 Cyg were solved by Lavrov's direct method, and elements of the photometric orbit were calculated. The results obtained in R-curve are different from data for other ones. We estimated the absolute basic parameters of the components: M1=2.0M⊙, M2 = 1.7M⊙, R1=1.7R⊙, R2 = 1.6R⊙, M1, bol = +1.7m, M2,bol = +2.5m. The components do not reach ZAMS in the H-R diagram and their age is 107 yrs. The distance for the star V456 Cyg is about 600 pc. The star lies in a well-maked OB-stars group separated by a distance of about 500 pc in the direction of the OB-association Cyg OB1.

  13. Study of Eclipsing Binary and Multiple Systems in OB Associations IV: Cas OB6 Member DN Cas

    NASA Astrophysics Data System (ADS)

    Bakış, V.; Bakış, H.; Bilir, S.; Eker, Z.

    2016-09-01

    An early-type, massive, short-period (Porb=2d.310951) eclipsing spectroscopic binary DN Cas has been re-visited with new spectral and photometric data. The masses and radii of the components have been obtained as M1=19.04± 0.07 M⊙, M2=13.73± 0.05 M⊙ and R1=7.22± 0.06 R⊙, R2=5.79± 0.06 R⊙, respectively. Both components present synchronous rotation (Vrot1=160 km s-1, Vrot2=130 km s-1) with their orbit. Orbital period analysis yielded a physically bound additional component in the system with a minimum mass of M3=0.88 M⊙ orbiting in an eccentric orbit (e = 0.37 ± 0.2) with an orbital period of P 12 = 42 ± 9 yr. High precision absolute parameters of the system allowed us to derive a distance to DN Cas as 1.7 ± 0.2 kpc which locates the system within the borders of the Cas OB6 association (d = 1.8 kpc). The space velocities and the age of DN Cas are in agreement with those of Cas OB6. The age of DN Cas (τ = 3-5 Myr) is found to be 1-2 Myr older than the embedded clusters (IC 1795, IC 1805, and IC 1848) in the Cas OB6 association, which implies a sequential star formation in the association.

  14. Pre- and postprandial expression of the leptin receptor splice variants OB-Ra and OB-Rb in murine peripheral tissues.

    PubMed

    Frühbeck, G; Gómez-Ambrosi, J; Martínez, J A

    1999-01-01

    Leptin receptors (OB-R) are widely distributed in peripheral tissues. However, the RT-PCR data published on the distribution of OB-R are not always consistent. The present study was undertaken in order to test whether the different muscle fiber type profile or the acute nutritional status in which tissue samples were excised from animals may influence OB-R expression. Six 12-week-old male Swiss-Webster mice were killed by decapitation either 1 h after feeding or after a 16-h fast, and the kidneys, testes, brown adipose tissue, gastrocnemius (G), soleus (SOL) and extensor digitorum longus (EDL) muscles were dissected out. In parallel, muscle fibers obtained from other animals were classified on the basis of differences in the staining intensity for myofibrillar adenosinetriphosphatase. The expression of OB-R isoforms was assessed by RT-PCR and ethidium bromide staining. The signal for OB-Ra and OB-Rb was detected in all tissues examined. No differences were observed in samples obtained from either fed or fasted mice. G, SOL and EDL muscles showed the same pattern of OB-R expression. Neither the short-term nutritional changes of the animal as regards to the pre- versus the postprandial-state nor differences in muscle fiber type had any influence on the qualitative expression of the OB-R splice variants a and b in the murine tissues studied. However, quantitative differences cannot be ruled out.

  15. Beneficial Effects of Supplementation of the Rare Sugar "D-allulose" Against Hepatic Steatosis and Severe Obesity in Lep(ob)/Lep(ob) Mice.

    PubMed

    Itoh, Kouichi; Mizuno, Shodo; Hama, Sayuri; Oshima, Wataru; Kawamata, Miku; Hossain, Akram; Ishihara, Yasuhiro; Tokuda, Masaaki

    2015-07-01

    A rare sugar, D-allulose (also called D-psicose), has recently been applied as a food supplement in view of controlling diabetes and obesity in Japan. D-allulose has been proven to have unique effects against hyperglycemia and hyperlipidemia in a number of studies using several species of rats and mice. However, the antiobesity effects of D-allulose have not yet been assessed in Lep(ob)/Lep(ob) (ob/ob) mice. Therefore, this study explored the dietary supplemental effects of this sugar in leptin-deficient ob/ob mice. Consequently, the subchronic ingestion of D-allulose in ob/ob mice for 15 wk significantly decreased the body and liver weights, and the loss of body weight was involved in the reduction of the total fat mass, including abdominal visceral fat, and not fat-free body mass, including muscle. Furthermore, D-allulose improved hepatic steatosis, as evaluated using hepatic histological studies and MRI. In the normal mice, none of these parameters were influenced by the single or long-term ingestion of D-allulose. These results indicate that dietary supplementation of D-allulose especially influences postprandial hyperglycemia and obesity-related hepatic steatosis, without exercise therapy or dietary restriction. Therefore, D-allulose may be useful as a supplement for preventing and improving obesity and obesity-related disorders.

  16. Long-term administration of PACAP receptor antagonist, PACAP(6-27), impairs glucose tolerance and insulin sensitivity in obese diabetic ob/ob mice.

    PubMed

    Green, Brian D; Irwin, Nigel; Cassidy, Roslyn S; Gault, Victor A; Flatt, Peter R

    2006-09-01

    Pituitary adenylate cyclase-activating peptide (PACAP) is a ubiquitous peptide of the glucagon superfamily that is involved in glucose homeostasis and regulation of insulin secretion. This study employed the PACAP receptor antagonist, PACAP(6-27) to evaluate the role of endogenous PACAP in genetic obesity-related diabetes and related metabolic abnormalities using ob/ob mice. Acute in vivo antagonistic potency of PACAP(6-27) was confirmed in ob/ob mice by blockade of the insulin-releasing action but not hyperglycaemia. In longer-term studies, ob/ob mice were given once daily injections of PACAP(6-27) or vehicle for 14 days. Feeding activity, body weight, basal plasma glucose and plasma insulin concentrations were not significantly affected by chronic PACAP(6-27) treatment. However, PACAP(6-27) treatment impaired glucose tolerance, insulin sensitivity and the glycaemic response to feeding. Plasma glucagon and lipids were unchanged. These observations indicate a role of endogenous PACAP for normal glucose homeostasis, but indicate a minor involvement in the regulation of insulin secretion in ob/ob mice.

  17. Hepatic Macrosteatosis Is Partially Converted to Microsteatosis by Melatonin Supplementation in ob/ob Mice Non-Alcoholic Fatty Liver Disease

    PubMed Central

    Lavazza, Antonio; Golic, Igor; Aleksic, Marija; Korac, Aleksandra; Rodella, Luigi Fabrizio; Rezzani, Rita

    2016-01-01

    Background Obesity is a common risk factor for non-alcoholic fatty liver disease (NAFLD). Currently, there are no specific treatments against NAFLD. Thus, examining any molecule with potential benefits against this condition emerged melatonin as a molecule that influences metabolic dysfunctions. The aim of this study was to determine whether melatonin would function against NAFDL, studying morphological, ultrastuctural and metabolic markers that characterize the liver of ob/ob mice. Methods Lean and ob/ob mice were supplemented with melatonin in the drinking water for 8 weeks. Histology and stereology were performed to assess hepatic steatosis and glycogen deposition. Ultrastructural features of mitochondria, endoplasmic reticulum (ER) and their juxtapositions were evaluated in livers of all experimental groups. Furthermore, hepatic distribution and expression of markers of ER and mitochondria (calnexin, ATP sintase β, GRP78 and CHOP) and metabolic dysfunction (RPB4, β-catenin) and cellular longevity (SIRT1) were analyzed. Results Melatonin significantly reduced glycemia, identified also by a decrease of hepatic RBP4 expression, reversed macrosteatosis in microsteatosis at the hepatic pericentral zone, enlarged ER-mitochondrial distance and ameliorated the morphology and organization of these organelles in ob/ob mouse liver. Furthermore, in ob/ob mice, calnexin and ATP synthase β were partially restored, GRP78 and CHOP decreased in periportal and midzonal hepatocytes and β-catenin expression was, in part, restored in peripheral membranes of hepatocytes. Melatonin supplementation to ob/ob mice improves hepatic morphological, ultrastructural and metabolic damage that occurs as a result of NAFLD. Conclusions Melatonin may be a potential adjuvant treatment to limit NAFLD and its progression into irreversible complications. PMID:26824477

  18. Leptin deficient ob/ob mice and diet-induced obese mice responded differently to Roux-en-Y bypass surgery.

    PubMed

    Hao, Z; Münzberg, H; Rezai-Zadeh, K; Keenan, M; Coulon, D; Lu, H; Berthoud, H-R; Ye, J

    2015-05-01

    Weight regain contributes to the therapeutic failure in 15-20% of type 2 diabetic patients after Roux-en-Y gastric bypass surgery (RYGB), and the mechanism remains largely unknown. This study was conducted to explore the mechanism of weight regain. Wild-type (WT) diet-induced obese (DIO) mice were used to mimic human obesity, and ob/ob mice were used for leptin deficiency-induced obesity. Two groups of mice were compared in weight regain for 10 months after RYGB. Weight loss, food intake, fecal energy loss and energy expenditure were monitored in the study of weight regain. Fasting insulin, insulin tolerance and homeostatic model assessment-insulin resistance were tested for insulin sensitivity under the weight regain. Weight loss from RYGB and calorie restriction was compared for the impact in insulin sensitivity. In WT mice, RYGB induced a sustained weight loss and insulin sensitization over the sham operation in this 10-month study. However, RYGB failed to generate the same effects in leptin-deficient ob/ob mice, which suffered a weight regain over the pre-surgery level. In ob/ob mice, body weight was reduced by RYGB transiently in the first week, recovered in the second week and increased over the baseline thereafter. Weight loss was induced by RYGB relative to that of sham mice, but the loss was not sufficient to keep body weight below the pre-surgery levels. In addition, insulin sensitivity was not improved by the weight loss. The response to RYGB was improved in ob/ob mice by 2 weeks of leptin treatment. Weight loss from calorie restriction had an equivalent effect on insulin sensitization compared with that of RYGB. Those data demonstrate that ob/ob mice and DIO mice responded differently to RYGB surgery, suggesting that leptin may be one of the factors required for RYGB to prevent weight regain and diabetes recurrence.

  19. Leptin deficient ob/ob mice and diet-induced obese mice responded differently to Roux-en-Y bypass surgery

    PubMed Central

    Hao, Z; Münzberg, H; Rezai-Zadeh, K; Keenan, M; Coulon, D; Lu, H; Berthoud, H-R; Ye, J

    2015-01-01

    OBJECTIVE Weight regain contributes to the therapeutic failure in 15–20% of type 2 diabetic patients after Roux-en-Y gastric bypass surgery (RYGB), and the mechanism remains largely unknown. This study was conducted to explore the mechanism of weight regain. RESEARCH DESIGN Wild-type (WT) diet-induced obese (DIO) mice were used to mimic human obesity, and ob/ob mice were used for leptin deficiency-induced obesity. Two groups of mice were compared in weight regain for 10 months after RYGB. Weight loss, food intake, fecal energy loss and energy expenditure were monitored in the study of weight regain. Fasting insulin, insulin tolerance and homeostatic model assessment-insulin resistance were tested for insulin sensitivity under the weight regain. Weight loss from RYGB and calorie restriction was compared for the impact in insulin sensitivity. RESULTS In WT mice, RYGB induced a sustained weight loss and insulin sensitization over the sham operation in this 10-month study. However, RYGB failed to generate the same effects in leptin-deficient ob/ob mice, which suffered a weight regain over the pre-surgery level. In ob/ob mice, body weight was reduced by RYGB transiently in the first week, recovered in the second week and increased over the baseline thereafter. Weight loss was induced by RYGB relative to that of sham mice, but the loss was not sufficient to keep body weight below the pre-surgery levels. In addition, insulin sensitivity was not improved by the weight loss. The response to RYGB was improved in ob/ob mice by 2 weeks of leptin treatment. Weight loss from calorie restriction had an equivalent effect on insulin sensitization compared with that of RYGB. CONCLUSION Those data demonstrate that ob/ob mice and DIO mice responded differently to RYGB surgery, suggesting that leptin may be one of the factors required for RYGB to prevent weight regain and diabetes recurrence. PMID:25349056

  20. Development of Hydrophone and Its Aapplications Use in OBS

    NASA Astrophysics Data System (ADS)

    Lin, C. R.; Kuo, B. Y.; Wang, C. C.; Chang, H. K.; Jang, J. P.

    2014-12-01

    The seismic wave is translated to T-wave when earthquakes occur below seafloor with a traveling speed of 1500 m/s in the SOFAR channel which centers roughly at 1000 m water depths (Tolstoy and Ewing, 1950). Many studies have shown that hydrophone can be set up in the SOFAR channel to collect acoustic data to investigate plate movement (Fox and Dziak, 1999), seismisity (Fox et.al, 2001), characteristic of T-wave (Park et.al, 2001), seafloor volcanism (Nicolas, 1989), among others. Institute of Earth Sciences of Academia Sinica has been collaborating with Taiwan Ocean Research Institute (TORI) and the Institute of Undersea Technology (IUT) of the National Sun Yat-sen University (NSYSU) to succefully build a new wide-band OBS (Yardbird). We hope that Yardbird is not only an OBS but also a multi-function underwater recording plotform. Therefor we are developing a low-power consumption, inexpensive hydrophone to catch acoustic signals. The protoypte of it has passed a series of tests and got some seismic seafloor data. This paper describes the design goal of hydrophone, component specifications, field data analyzed, and discusses the future directions of instrument development. Keywords: T-wave; Hydrophone; OBS; acoustic signal Referance: 1 Fox, C. G. and R. P. Dziak, Intenal deformation of the Gorda Plate observedb y hydroacoustimc onitoring, J . Geophys Res., 104, 17603-17615, 1999. 2 Fox, C. G., H. Matsumoto and T.K.Lau, Monitoring Pacific Ocean seismicity from an autonomous hydrophone array. , J . Geophys. Res., 106,4183-4206, 2001. 3 Nicolas, A., Structures of ophiolites and dynamics of oceanic lithosphere. Dordrecht ; Boston : Kluwer Academic Publishers, 1989. 4. Park, M., R.I. Odom, and D.J. Soukup (2001). Modal scattering: a key to understanding oceanic T-waves, Geophys. Res. Lett. 28, 3401-3404. 5. Tolstoy, I. and M. Ewing (1950). The T phase of shallow-focus earthquakes, Bull. Seismol. Soc. Am. 40, 25-51.

  1. OBS FOMAR POOL: Gibraltar and ALERTES-RIM experiments.

    NASA Astrophysics Data System (ADS)

    Pazos, Antonio; Martín Davila, Jose; Buforn, Elisa; Cabieces, Roberto; Santos, Jose; Sandoval, Nicolas; Roca, Antoni; Dahm, Torsten

    2016-04-01

    The Eurasian-African plate boundary crosses the called "Ibero-Maghrebian" region from the San Vicente Cape (SW Portugal) to Tunisia including the south Iberia, Alboran Sea, and northern of Morocco and Algeria. The low convergence rate at this plate boundary produces a continuous moderate seismic activity of low magnitude and shallow depth, where the occurrence of large earthquakes is separated by long time intervals, even with associated tsunamis, like the 1755 Lisbon earthquake. In this region, there are also intermediate and very deep earthquakes. Due to the fact that part of the seismic activity is located at marine areas, and also because of the poor geographic azimuthal coverage at some zones provided by the land stations (specially in the SW of the San Vicente Cape area), Royal Spanish Navy Observatory (ROA) acquired three "LOSTERN" broad band (CMG-40T sensors) OBS, manufactured by KUM (Kiel, Germany), and, more recently (2014), the Complutense University of Madrid (UCM) acquired another three with Trillium 120 sensors. All of them conform the OBS FOMAR pool. Since January to November 2014, the FOMAR pool has been deployed along the Gibraltar strait (Gibraltar experiment), in collaboration with SECEGSA (Spanish society to study the fix communication through the Gibraltar Strait), to study the local microseismicity in the Gibraltar strait area. Also, since September 2015, the FOMAR pool has been deployed for 8 months in SW of the San Vicente Cape with an hexagonal array configuration as a part of ALERTES-RIM project. In this work the some preliminary results of the Gibraltar strait and ALERTES-RIM OBS experiment are shown.

  2. The distance to the Galactic center determined by OB stars

    NASA Astrophysics Data System (ADS)

    Branham, Richard L.

    2014-09-01

    The OB stars are concentrated near the Galactic plane and should permit a determination of the distance to the Galactic center. van Leeuwen's new reduction of the Hipparcos catalog provides, after 824 Gould belt stars have been excluded, 6288 OB stars out to 1 kpc and Westin's compilation an additional 112 stars between 1 kpc and 3 kpc. The reduction model involves 14 unknowns: the Oort A and B constants, the distance to the Galactic center R 0, 2 second-order partial derivatives, the 3 components of solar motion, a K term, a first order partial derivative for motion perpendicular to the Galactic plane, a second-order partial for acceleration perpendicular to the plane, two terms for a possible expansion of the OB stars, and a C constant. The model is nonlinear, and the unknowns are calculated by use the simplex algorithm for nonlinear adjustment applied to 14313 equations of condition, 12694 in proper motion and 1619 in radial velocity. Various solutions were tried: an L1 solution, a least squares solution with modest (2.7 %) trim of the data, and two robust least squares solutions (biweight and Welsch weighting) with more extreme trimming. The Welsch solution seems to give the best results and calculates a distance to the Galactic center 6.72±0.39 kpc. Statistical tests show that the data are homogeneous, that the reduction model seems adequate and conforms with the assumptions used in its derivation, and that the post-fit residuals are random. Inclusion of more terms, such as streaming motion induced by Galactic density waves, degrades the solution.

  3. Determining OBS Instrument Orientations: A Comparison of Algorithms

    NASA Astrophysics Data System (ADS)

    Doran, A. K.; Laske, G.

    2015-12-01

    The alignment of the orientation of the horizontal seismometer components with the geographical coordinate system is critical for a wide variety of seismic analyses, but the traditional deployment method of ocean bottom seismometers (OBS) precludes knowledge of this parameter. Current techniques for determining the orientation predominantly rely on body and surface wave data recorded from teleseismic events with sufficiently large magnitudes. Both wave types experience lateral refraction between the source and receiver as a result of heterogeneity and anisotropy, and therefore the arrival angle of any one phase can significantly deviate from the great circle minor arc. We systematically compare the results and uncertainties obtained through current determination methods, as well as describe a new algorithm that uses body wave, surface wave, and differential pressure gauge data (where available) to invert for horizontal orientation. To start with, our method is based on the easily transportable computer code of Stachnik et al. (2012) that is publicly available through IRIS. A major addition is that we utilize updated global dispersion maps to account for lateral refraction, as was done by Laske (1995). We also make measurements in a wide range of frequencies, and analyze surface wave trains of repeat orbits. Our method has the advantage of requiring fewer total events to achieve high precision estimates, which is beneficial for OBS deployments that can be as short as weeks. Although the program is designed for the purpose of use with OBS instruments, it also works with standard land installations. We intend to provide the community with a program that is easy to use, requires minimal user input, and is optimized to work with data cataloged at the IRIS DMC.

  4. Application of the full waveform inversion to OBS data sets

    NASA Astrophysics Data System (ADS)

    Lee, D.; Chung, W.; Shin, S.; Lim, C.; Kwon, J.

    2016-12-01

    Full Waveform Inversion technology is the most active area of research in the field of seismic data processing as of recent. A model data is created through numerical modeling by assuming the velocity model of a certain subsurface medium; then, the model data and the observed data obtained from the seismic prospecting are compared by setting the objective function. The process of calculating the gradient direction with the minimum objective function and renewing the velocity model of the subsurface medium in the gradient direction is repeated. When the objective function reaches a certain threshold value, the iterative calculation is terminated. Such automated process is called waveform inversion, and through this method, the inferred velocity model of the subsurface medium can be assumed through the velocity distribution of the underground medium in which the actual prospecting was conducted. Most of the waveform inversions use a wave equation which assumes the acoustic medium, and in the case of using the elastic wave data obtained on land, use a wave equation which assumes elastic medium. According to the recent development of prospecting equipment, the acquisition of OBS data, a multicomponent data, is frequently carried out in the oceans as well. In order to effectively process the OBS data obtained in the ocean, the characteristics of acoustic medium and elastic medium should be concurrently considered, and to this end, a wave equation at the acoustic-elastic coupled media or a staggered-grid finite-difference method(FDM) can be applied. In this study, we developed a waveform inversion algorithm that can invert the OBS data based on the a staggered-grid FDM, and verified the inversion result by using the Marmousi 2 model with a small number of geophones, revised as shown in Fig. 1. Through this, we confirmed that the velocities of P wave and S wave could be estimated by applying OBS data to the waveform inversion. Also, it is deemed that in the future, the

  5. The Dynamics of Star Clusters and OB Associations

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas

    2017-06-01

    The formation and evolution of star clusters is fundamental to our understanding of star formation, the evolution of binary and planetary systems, and the formation of long-lived open and globular clusters. Our understanding of these systems is being revolutionised by new data from Gaia and large-scale RV surveys. I will present multiple kinematic studies of star clusters and OB associations that constrain their kinematic state, reveal evidence for cluster expansion and dispersal, expose considerable kinematic substructure, and provide observations of the disruption of binary systems.

  6. A Search for Pulsar Companions to OB Runaway Stars

    NASA Technical Reports Server (NTRS)

    Kaspi, V. M.

    1995-01-01

    We have searched for radio pulsar companions to 40 nearby OB runaway stars. Observations were made at 474 and 770 MHz with the NRAO 140 ft telescope. The survey was sensitive to long- period pulsars with flux densities of 1 mJy or more. One previously unknown pulsar was discovered, PSRJ2044+4614, while observing towards target O star BD+45,3260. Follow-up timing observations of the pulsar measured its position to high precision, revealing a 9' separation between the pulsar and the target star, unequivocally indicating they are not associated.

  7. A New Subdwarf-OB Pulsator J23341+4622

    NASA Astrophysics Data System (ADS)

    Pakštiene, E.; Qvam, J. K. T.; Østensen, R. H.; Telting, J. H.

    2014-12-01

    A new sdOB variable star, J23341+4622 (SDSS J233406.10+462249.3), was discovered during photometric observations with the Nordic Optical Telescope (La Palma). We found its main pulsation frequency at 7422(±9) μHz with an amplitude of 4.5(±0.5) mma. The star is possibly a complex pulsator, as we found another significant peak at 7759(±11) μHz with an amplitude of 2.0(±0.3) mma and a possible subharmonic of the main frequency at 3508 μHz with an amplitude of 2.3 mma.

  8. Leptin stimulates bone formation in ob/ob mice at doses having minimal impact on energy metabolism.

    PubMed

    Philbrick, Kenneth A; Wong, Carmen P; Branscum, Adam J; Turner, Russell T; Iwaniec, Urszula T

    2017-03-01

    Leptin, the protein product of the ob gene, is essential for normal bone growth, maturation and turnover. Peripheral actions of leptin occur at lower serum levels of the hormone than central actions because entry of leptin into the central nervous system (CNS) is limited due to its saturable transport across the blood-brain barrier (BBB). We performed a study in mice to model the impact of leptin production associated with different levels of adiposity on bone formation and compared the response with well-established centrally mediated actions of the hormone on energy metabolism. Leptin was infused (0, 4, 12, 40, 140 or 400 ng/h) for 12 days into 6-week-old female ob/ob mice (n = 8/group) using sc-implanted osmotic pumps. Treatment resulted in a dose-associated increase in serum leptin. Bone formation parameters were increased at EC50 infusion rates of 7-17 ng/h, whereas higher levels (EC50, 40-80 ng/h) were required to similarly influence indices of energy metabolism. We then analyzed gene expression in tibia and hypothalamus at dose rates of 0, 12 and 140 ng/h; the latter dose resulted in serum leptin levels similar to WT mice. Infusion with 12 ng/h leptin increased the expression of genes associated with Jak/Stat signaling and bone formation in tibia with minimal effect on Jak/Stat signaling and neurotransmitters in hypothalamus. The results suggest that leptin acts peripherally to couple bone acquisition to energy availability and that limited transport across the BBB insures that the growth-promoting actions of peripheral leptin are not curtailed by the hormone's CNS-mediated anorexigenic actions. © 2017 Society for Endocrinology.

  9. Dietary Capsaicin Improves Glucose Homeostasis and Alters the Gut Microbiota in Obese Diabetic ob/ob Mice.

    PubMed

    Song, Jun-Xian; Ren, Hui; Gao, Yuan-Feng; Lee, Chong-You; Li, Su-Fang; Zhang, Feng; Li, Long; Chen, Hong

    2017-01-01

    Background: The effects of capsaicin on obesity and glucose homeostasis are still controversial and the mechanisms underlying these effects remain largely unknown. This study aimed to investigate the potential relationship between the regulation of obesity and glucose homeostasis by dietary capsaicin and the alterations of gut microbiota in obese diabetic ob/ob mice. Methods: The ob/ob mice were subjected to a normal, low-capsaicin (0.01%), or high-capsaicin (0.02%) diet for 6 weeks, respectively. Obesity phenotypes, glucose homeostasis, the gut microbiota structure and composition, short-chain fatty acids, gastrointestinal hormones, and pro-inflammatory cytokines were measured. Results: Both the low- and high-capsaicin diets failed to prevent the increase in body weight, adiposity index, and Lee's obesity index. However, dietary capsaicin at both the low and high doses significantly inhibited the increase of fasting blood glucose and insulin levels. These inhibitory effects were comparable between the two groups. Similarly, dietary capsaicin resulted in remarkable improvement in glucose and insulin tolerance. In addition, neither the low- nor high-capsaicin diet could alter the α-diversity and β-diversity of the gut microbiota. Taxonomy-based analysis showed that both the low- and high-capsaicin diets, acting in similar ways, significantly increased the Firmicutes/Bacteroidetes ratio at the phylum level as well as increased the Roseburia abundance and decreased the Bacteroides and Parabacteroides abundances at the genus level. Spearman's correlation analysis revealed that the Roseburia abundance was negatively while the Bacteroides and Parabacteroides abundances were positively correlated to the fasting blood glucose level and area under the curve by the oral glucose tolerance test. Finally, the low- and high-capsaicin diets significantly increased the fecal butyrate and plasma total GLP-1 levels, but decreased plasma total ghrelin, TNF-α, IL-1β, and IL-6

  10. The Seismic Broad Band Western Mediterranean (wm) Network and the Obs Fomar Pool: Current state and Obs activities.

    NASA Astrophysics Data System (ADS)

    Pazos, Antonio; Davila, Jose Martin; Buforn, Elisa; Bezzeghoud, Mourad; Harnafi, Mimoun; Mattesini, Mauricio; Caldeira, Bento; Hanka, Winfried; El Moudnib, Lahcen; Strollo, Angelo; Roca, Antoni; Lopez de Mesa, Mireya; Dahm, Torsten; Cabieces, Roberto

    2016-04-01

    The Western Mediterranean (WM) seismic network started in 1996 as an initiative of the Royal Spanish Navy Observatory (ROA) and the Universidad Complutense de Madrid (UCM), with the collaboration of the GeoForschungsZentrum (GFZ) of Potsdam. A first broad band seismic station (SFUC) was installed close to Cádiz (South Spain). Since then, additional stations have been installed in the Ibero-Moghrebian region. In 2005, the "WM" code was assigned by the FDSN and new partners were jointed: Evora University (UEVO, Portugal), the Scientifique Institute of Rabat (ISRABAT, Morocco), and GFZ. Now days, the WM network is composed by 15 BB stations, all of them with Streckaisen STS-2 or STS-2.5 sensors, Quanterra or Earthdata digitizers and SeiscomP. Most them have co-installed a permanent geodetic GPS stations, and some them also have an accelerometer. There are 10 stations deployed in Spanish territory (5 in the Iberian peninsula, 1 in Balearic islands and 4 in North Africa Spanish places) with VSAT or Internet communications, 2 in Portugal (one of them without real time), and 3 in Morocco (2 VSAT and 1 ADSL). Additionally, 2 more stations (one in South Spain and one in Morocco) will be installed along this year. Additionally ROA has deployed a permanent real time VBB (CMG-3T: 360s) station at the Alboran Island. Due to the fact that part of the seismic activity is located at marine areas, and also because of the poor geographic azimuthal coverage at some zones provided by the land stations (specially in the SW of the San Vicente Cape area), ROA and UCM have acquired six broad band "LOBSTERN" OBS, manufactured by KUM (Kiel, Germany), conforming the OBS FOMAR pool. Three of them with CMG-40T sensor and the other with Trillium 120. These OBS were deployed along the Gibraltar strait since January to November 2014 to study the microseismicity in the Gibraltar strait area. In September 2015 FOMAR network has been deployed in SW of the San Vicente Cape for 8 months as a part of

  11. The effect of leptin replacement on sleep-disordered breathing in the leptin-deficient ob/ob mouse.

    PubMed

    Pho, H; Hernandez, A B; Arias, R S; Leitner, E B; Van Kooten, S; Kirkness, J P; Schneider, H; Smith, P L; Polotsky, V Y; Schwartz, A R

    2016-01-01

    Obese leptin-deficient (ob/ob) mice demonstrate defects in upper airway structural and neuromuscular control. We hypothesized that these defects predispose to upper airway obstruction during sleep, and improve with leptin administration. High-fidelity polysomnographic recordings were conducted to characterize sleep and breathing patterns in conscious, unrestrained ob/ob mice (23 wk, 67.2 ± 4.1 g, n = 13). In a parallel-arm crossover study, we compared responses to subcutaneous leptin (1 μg/h) vs. vehicle on respiratory parameters during NREM and REM sleep. Upper airway obstruction was defined by the presence of inspiratory airflow limitation (IFL), as characterized by an early inspiratory plateau in airflow at a maximum level (V̇Imax) with increasing effort. The severity of upper airway obstruction (V̇Imax) was assessed along with minute ventilation (V̇E), tidal volume (VT), respiratory rate (RR), inspiratory duty cycle, and mean inspiratory flow at each time point. IFL occurred more frequently in REM sleep (37.6 ± 0.2% vs. 1.1 ± 0.0% in NREM sleep, P < 0.001), and leptin did not alter its frequency. V̇Imax (3.7 ± 1.1 vs. 2.7 ± 0.8 ml/s, P < 0.001) and V̇E increased (55.4 ± 22.0 vs. 39.8 ± 16.4 ml/min, P < 0.001) with leptin vs. vehicle administration. The increase in V̇E was due to a significant increase in VT (0.20 ± 0.06 vs. 0.16 ± 0.05 ml, P < 0.01) rather than RR. Increases in V̇E were attributable to increases in mean inspiratory flow (2.5 ± 0.8 vs. 1.8 ± 0.6 ml/s, P < 0.001) rather than inspiratory duty cycle. Similar increases in V̇E and its components were observed in non-flow-limited breaths during NREM and REM sleep. These responses suggest that leptin stabilized pharyngeal patency and increased drive to both the upper airway and diaphragm during sleep.

  12. The effect of leptin replacement on sleep-disordered breathing in the leptin-deficient ob/ob mouse

    PubMed Central

    Pho, H.; Hernandez, A. B.; Arias, R. S.; Leitner, E. B.; Van Kooten, S.; Kirkness, J. P.; Schneider, H.; Smith, P. L.; Polotsky, V. Y.

    2015-01-01

    Obese leptin-deficient (ob/ob) mice demonstrate defects in upper airway structural and neuromuscular control. We hypothesized that these defects predispose to upper airway obstruction during sleep, and improve with leptin administration. High-fidelity polysomnographic recordings were conducted to characterize sleep and breathing patterns in conscious, unrestrained ob/ob mice (23 wk, 67.2 ± 4.1 g, n = 13). In a parallel-arm crossover study, we compared responses to subcutaneous leptin (1 μg/h) vs. vehicle on respiratory parameters during NREM and REM sleep. Upper airway obstruction was defined by the presence of inspiratory airflow limitation (IFL), as characterized by an early inspiratory plateau in airflow at a maximum level (V̇imax) with increasing effort. The severity of upper airway obstruction (V̇imax) was assessed along with minute ventilation (V̇e), tidal volume (VT), respiratory rate (RR), inspiratory duty cycle, and mean inspiratory flow at each time point. IFL occurred more frequently in REM sleep (37.6 ± 0.2% vs. 1.1 ± 0.0% in NREM sleep, P < 0.001), and leptin did not alter its frequency. V̇imax (3.7 ± 1.1 vs. 2.7 ± 0.8 ml/s, P < 0.001) and V̇e increased (55.4 ± 22.0 vs. 39.8 ± 16.4 ml/min, P < 0.001) with leptin vs. vehicle administration. The increase in V̇e was due to a significant increase in VT (0.20 ± 0.06 vs. 0.16 ± 0.05 ml, P < 0.01) rather than RR. Increases in V̇e were attributable to increases in mean inspiratory flow (2.5 ± 0.8 vs. 1.8 ± 0.6 ml/s, P < 0.001) rather than inspiratory duty cycle. Similar increases in V̇e and its components were observed in non-flow-limited breaths during NREM and REM sleep. These responses suggest that leptin stabilized pharyngeal patency and increased drive to both the upper airway and diaphragm during sleep. PMID:26472867

  13. A novel technique for resolving burst contention in OBS networks

    NASA Astrophysics Data System (ADS)

    Garg, Amit Kumar; Kaler, R. S.

    2010-03-01

    In this article, in order to resolve burst contention (burst dropping), a new scheme for an optical burst switching (OBS) network has been proposed which is based on the integration of wavelength selection and burst assignment. The proposed scheme provides proportional differentiated services in bufferless OBS networks by dynamically assigning more and longer periods of wavelengths to higher priority classes. In addition, burst head packets (BHPs) are buffered electrically so that fiber delay lines are unnecessary at core nodes. As a result, BHP of a lower priority class is buffered at the core router when it cannot find an available wavelength. It has an opportunity to reschedule its burst to the wavelengths that have been assigned to higher priority classes but have not yet been reserved. The proposed integrated scheme not only provides proportional differentiated services but also achieves lower average dropping probability without any preemption or segmentation mechanisms. Compared with existing approaches, the proposed integrated scheme does not need to generate any special packets and needs to maintain only a few parameters at core nodes.The proposed scheme has several attractive features such as fast, simple implementation and improved burst dropping performance. Extensive simulation results have been used to demonstrate the effectiveness of the proposed scheme in comparison to traditional schemes.

  14. Analysis of methane biodegradation by Methylosinus trichosporium OB3b

    PubMed Central

    Rodrigues, Andréa dos Santos; Salgado, Belkis Valdman e Andréa Medeiros

    2009-01-01

    The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h-1, 0.6830 gcells/gCH4, 73.73% and 2.7732.10-3 gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated. PMID:24031362

  15. A new Shallow Water Cabled OBS System off California

    NASA Astrophysics Data System (ADS)

    Rademacher, Horst; Pearcey, Chris; Mangano, Giorgio; Guralp, Cansun; Pearce, Nathan

    2014-05-01

    During the summer and fall of 2013 we installed a turnkey cabled network of four combination broadband velocity/acceleration ocean bottom sensors (OBS) on the sea floor near Point Buchon in the Northeastern Pacific Ocean off the coast of Central California. We implemented a novel network design by daisy-chaining the instruments to one single multistranded cable. The signals of each station are digitized in-situ and then transmitted via dedicated optical fiber links inside the cable to a shore station. From there they are fed in real time via a cell phone modem into several seismic networks in California. The goal of this dense network is to monitor the microseismicity of two offshore faults running parallel to the strike of the San Andreas Fault. However, because the network is installed in rather shallow water near the coast, the action of waves and swell at the sea surface affect the sensor registrations much stronger as compared to the typical deep water installation of OBS equipment. We will report about the challenges of installing and maintaining the network and present some initial results.

  16. Microseismicity of the frontal Hellenic arc according to OBS observations

    NASA Astrophysics Data System (ADS)

    Kovachev, S. A.; Kuzin, I. P.; Soloviev, S. L.

    1992-01-01

    Five ocean bottom seismographs (OBS) of the pop-up and tethered types were installed southeast of the island of Crete at depth ranging from 1700 to 4000 m, and worked continuously for one week (July 2-9, 1988). Some one thousand microearthquakes with local magnitude ML from approximately 0 to 1.9 were registered. A total of 105 quakes were recorded by three or more instruments. Foci of these microearthquakes in the earth's crust and upper mantle are concentrated off the eastern flank of Crete along the contact between two blocks of the Hellenic arc. The en-echelon arrangement of the Pliny and Strabo deep-water trenches to the south of Crete is accompanied by a twofold Benioff zone. The seismic zones dip under the Hellenic arc with the same angle of 60° and are also en-echelon. The Strabo zone is probably the younger with a maximum focal depth of 70 km against 120 km for Pliny zone. Seismic activity in the crust beneath the island is revealed to extend along the entire slope of the Pliny trench, but there is a principal maximum of activity at ±5 km, a minimum at 15-20 km, and a second maximum at 20-30 km. These findings are in agreement with results from OBS observations made to north of Crete in 1987.

  17. OT1_mrubio_1: Clumping in OB-star winds

    NASA Astrophysics Data System (ADS)

    Rubio, M.

    2010-07-01

    Massive stars, their nature and evolution, play a important role at all stages of the Universe. Through their radiatively driven winds they influence on the dynamics and energetics of the interstellar medium. The winds of OB stars are the most studied case. Commonly, the mass-loss rates of luminous OB stars are inferred from several types of measurements, the strengths of UV P Cygni lines, H-alpha emission and radio and FIR continuum emission. Recent evidence indicates that currently accepted mass-loss rates may need to be revised downwards when small-scale density inhomogeneities (clumping) are taken into account. We argue that only a consistent treatment of ALL possible diagnostics, scanning different parts of the winds, and analyzed by means of state of the art model atmospheres, will permit the determination of true mass-loss rates. To this end we have assembled a variety of multi-wavelength data, but one crucial observational set is missing: far-IR diagnostics of free-free emission, which uniquely constrain the clumping properties of the wind at intermediate heights. We propose, therefore, to use PACS photometric mode to fill this crucial gap, studying the 70 and 110 micron fluxes of a carefully selected sample of 29 O4-B8 stars. These observations will provide the missing information to derive the clumping properties of the entire outflow, to understand the wind physics, and to obtain reliable massloss rates.

  18. OT2_mrubio_2: Clumping in OB-star winds

    NASA Astrophysics Data System (ADS)

    Rubio, M.

    2011-09-01

    Massive stars, their nature and evolution, play a important role at all stages of the Universe. Through their radiatively driven winds they influence on the dynamics and energetics of the interstellar medium. The winds of OB stars are the most studied case. Commonly, the mass-loss rates of luminous OB stars are inferred from several types of measurements, the strengths of UV P Cygni lines, H-alpha emission and radio and FIR continuum emission. Recent evidence indicates that currently accepted mass-loss rates may need to be revised downwards when small-scale density inhomogeneities (clumping) are taken into account. We argue that only a consistent treatment of ALL possible diagnostics, scanning different parts of the winds, and analyzed by means of state of the art model atmospheres, will permit the determination of true mass-loss rates. To this end we have assembled a variety of multi-wavelength data, but one crucial observational set is missing: far-IR diagnostics of free-free emission, which uniquely constrain the clumping properties of the wind at intermediate heights. We propose, therefore, to use PACS photometric mode to fill this crucial gap, studying the 70 and 110 micron fluxes of a carefully selected sample of 29 O4-B8 stars. These observations will provide the missing information to derive the clumping properties of the entire outflow, to understand the wind physics, and to obtain reliable massloss rates.

  19. Young Stars in the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    Hartigan, Patrick; Cleeves, Ilse

    2009-08-01

    Cygnus OB2 is by far the dominant region of massive star formation within 2-3 kpc of the Sun, but its stellar content is almost completely unknown owing to large amounts of visual extinction. Using NEWFIRM last fall we surveyed a large area of over two square degrees centered on the main cluster of O and B stars, and found the central part of the cluster to be teeming with at least several hundred, and probably over a thousand stars with near-IR excesses typical of circumstellar disks. With this proposal we aim to begin the process of acquiring spectral types for the entire sample of IR-excess and X-ray bright stars. This census will lead to masses, ages, and accretion rates for each object. With over 100 O stars, Cyg OB2 dwarfs the young clusters in Orion, and promises to provide a truly unique large sample of young stars that can address many of the major outstanding questions of star formation, such as IMF variations, binarity, triggering, mass segregation, accretion history, and disk evolution in a statistically significant way.

  20. Reduction of obesity, as induced by leptin, reverses endothelial dysfunction in obese (Lep(ob)) mice

    NASA Technical Reports Server (NTRS)

    Winters, B.; Mo, Z.; Brooks-Asplund, E.; Kim, S.; Shoukas, A.; Li, D.; Nyhan, D.; Berkowitz, D. E.

    2000-01-01

    Obesity is a major health care problem and is associated with significant cardiovascular morbidity. Leptin, a neuroendocrine hormone released by adipose tissue, is important in modulating obesity by signaling satiety and increasing metabolism. Moreover, leptin receptors are expressed on vascular endothelial cells (ECs) and mediate angiogenesis. We hypothesized that leptin may also play an important role in vasoregulation. We investigated vasoregulatory mechanisms in the leptin-deficient obese (ob/ob) mouse model and determined the influence of leptin replacement on endothelial-dependent vasorelaxant responses. The direct effect of leptin on EC nitric oxide (NO) production was also tested by using 4, 5-diaminofluorescein-2 diacetate staining and measurement of nitrate and nitrite concentrations. Vasoconstrictor responses to phenylephrine, norepinephrine, and U-46619 were markedly enhanced in aortic rings from ob/ob mice and were modulated by NO synthase inhibition. Vasorelaxant responses to ACh were markedly attenuated in mesenteric microvessels from ob/ob mice. Leptin replacement resulted in significant weight loss and reversal of the impaired endothelial-dependent vasorelaxant responses observed in ob/ob mice. Preincubation of ECs with leptin enhanced the release of NO production. Thus leptin-deficient ob/ob mice demonstrate marked abnormalities in vasoregulation, including impaired endothelial-dependent vasodilation, which is reversed by leptin replacement. These findings may be partially explained by the direct effect of leptin on endothelial NO production. These vascular abnormalities are similar to those observed in obese, diabetic, leptin-resistant humans. The ob/ob mouse may, therefore, be an excellent new model for the study of the cardiovascular effects of obesity.

  1. Variability Survey of Massive Stars in OB-Associations: Preliminary Results on the Cygnus Region

    NASA Astrophysics Data System (ADS)

    Laur, J.; Tuvikene, T.; Eenmäe, T.; Kolka, I.; Leedjärv, L.

    We present V- and I-passband photometry of massive stars in the Cyg OB1 and Cyg OB2 associations, based on about 80 observing nights spanning 300 days in the 2011 season. The variability of 22 supergiants and 48 OB-stars with luminosity classes III--V is analyzed. We report two new variable OB stars and 15 variable supergiants of which four are new discoveries. The light variations of Schulte 12 are interpreted as microvariability. We also present light curves of the red supergiants BC Cyg and BI Cyg which exhibit brightness drop of more than 0.4 mag during the season.

  2. Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice

    PubMed Central

    Hue, Jin-Joo; Lee, Ki Nam; Jeong, Jae-Hwang; Lee, Sang-Hwa; Lee, Young Ho; Jeong, Seong-woon; Nam, Sang Yoon; Yun, Young Won

    2009-01-01

    This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DG-CLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism. PMID:19687618

  3. Dietary Cooked Navy Beans and Their Fractions Attenuate Colon Carcinogenesis in Azoxymethane-Induced Ob/Ob Mice

    PubMed Central

    Bobe, Gerd; Barrett, Kathleen G.; Mentor-Marcel, Roycelynn A.; Saffiotti, Umberto; Young, Matthew R.; Colburn, Nancy H.; Albert, Paul S.; Bennink, Maurice R.; Lanza, Elaine

    2008-01-01

    Based on the protective effects of cooked dry bean consumption in a human intervention study, we evaluated which fraction of cooked dry beans is responsible for its cancer-preventive effects. Cooked navy beans (whole beans), the insoluble fraction (bean residue) or soluble fraction of the 60% (vol:vol) ethanol extract of cooked navy beans (bean extract), or a modified AIN-93G diet (16.6% fat including 12.9% lard) as control diet were fed to 160 male obese ob/ob mice after 2 azoxymethane injections. In comparison to control-fed mice, dysplasia, adenomas, or adenocarcinomas were detected in fewer mice on either bean fraction diet (percent reduction from control: whole beans 54%, P = 0.10; bean residue 81%, P = 0.003 ; bean extract 91%, P = 0.007) , and any type of colon lesions, including focal hyperplasia, were found in fewer mice on each of the 3 bean diets percent reduction from control: whole bean 56%, P = 0.04; bean residue 67%, P = 0.01; bean extract 87%, P = 0.0003. These results suggest that both the soluble and the insoluble fraction of the extract contribute to the cancer-protective effect of cooked navy beans. PMID:18444172

  4. Cosmography of OB stars in the solar neighbourhood

    NASA Astrophysics Data System (ADS)

    Bouy, H.; Alves, J.

    2015-12-01

    We construct a 3D map of the spatial density of OB stars within 500 pc from the Sun using the Hipparcos catalogue and find three large-scale stream-like structures that allow a new view on the solar neighbourhood. The spatial coherence of these blue streams and the monotonic age sequence over hundreds of parsecs suggest that they are made of young stars, similar to the young streams that are conspicuous in nearby spiral galaxies. The three streams are 1) the Scorpius to Canis Majoris stream, covering 350 pc and 65 Myr of star formation history; 2) the Vela stream, encompassing at least 150 pc and 25 Myr of star formation history; and 3) the Orion stream, including not only the well-known Orion OB1abcd associations, but also a large previously unreported foreground stellar group lying only 200 pc from the Sun. The map also reveals a remarkable and previously unknown nearby OB association, between the Orion stream and the Taurus molecular clouds, which might be responsible for the observed structure and star formation activity in this cloud complex. This new association also appears to be the birthplace of Betelgeuse, as indicated by the proximity and velocity of the red giant. If this is confirmed, it would solve the long-standing puzzle of the origin of Betelgeuse. The well-known nearby star-forming low-mass clouds, including the nearby T and R associations Lupus, Cha, Oph, CrA, Taurus, Vela R1, and various low-mass cometary clouds in Vela and Orion, appear in this new view of the local neighbourhood to be secondary star formation episodes that most likely were triggered by the feedback from the massive stars in the streams. We also recover well-known star clusters of various ages that are currently cruising through the solar neighbourhood. Finally, we find no evidence of an elliptical structure such as the Gould belt, a structure we suggest is a 2D projection effect, and not a physical ring. Table 3 is available in elctronic form at http://www.aanda.org

  5. The Ob Estuary (Kara Sea) Coastal Dynamics Interannual Variability Assessment

    NASA Astrophysics Data System (ADS)

    Kopa-Ovdienko, N.; Volobueva, N.; Ogorodov, S.

    2012-04-01

    There are several promising gas fields in the Ob Estuary. For their development comprehensive research, including coastal dynamics assessment, is required. To estimate current and speak about future coastal dynamics rates we need to investigate its interannual variability connecting it with variability of major coastal dynamics factors. It is known that in this region one of the main coastal dynamics factors is wave action, which, in turn, depends on wind velocity and direction. Wind data for ice-free period were derived from nearby hydro-meteorological stations. The research was conducted for two sections of the Ob Estuary coast. One section is located on the eastern coast of the estuary (Taz peninsula); the other is located on the western one (Yamal peninsula). Each of these sections is 10 km length. For key-sites of the coast wave-dangerous rhumbs were determined. For ice-free period of each year accumulated summer storm duration (ASSD) - number of hours with strong winds (≥10 m/s) from wave-dangerous directions - was calculated for both sections of coast. Supposing that coast retreat rate is proportional to number of hours with strong winds from wave-dangerous rhumbs, we can approximately assess interannual variability of coast retreat rate. Our research revealed that: 1)There are significant (from hours to a few hundreds of hours) differences in ASSD from year to year due to short ice-free period and high variability of wind velocity and direction. So, we can expect sharp interannual differences in coastal retreat rate. 2)The duration of strong winds from wave-dangerous rhumbs during ice-free period is more on the eastern coast of the estuary than on the western. This goes from the predominance of northern and northwestern winds during ice-free period. These winds cause waves leading to coastal erosion on the eastern coast of estuary and don't cause such waves on the western one. However, we can't draw conclusions about the comparative rates of coastal

  6. IFN-γ secretion in gut of Ob/Ob mice after vertical sleeve gastrectomy and its function in weight loss mechanism.

    PubMed

    Du, Jin-Peng; Wang, Geng; Hu, Chao-Jie; Wang, Qing-Bo; Li, Hui-Qing; Xia, Wen-Fang; Shuai, Xiao-Ming; Tao, Kai-Xiong; Wang, Guo-Bin; Xia, Ze-Feng

    2016-06-01

    Vertical sleeve gastrectomy (VSG) is becoming more and more popular among the world. Despite its dramatic efficacy, however, the mechanism of VSG remains largely undetermined. This study aimed to test interferon (IFN)-γ secretion n of mesenteric lymph nodes in obese mice (ob/ob mice), a model of VSG, and its relationship with farnesoid X receptor (FXR) expression in the liver and small intestine, and to investigate the weight loss mechanism of VSG. The wild type (WT) mice and ob/ob mice were divided into four groups: A (WT+Sham), B (WT+VSG), C (ob/ob+Sham), and D (ob/ob+VSG). Body weight values were monitored. The IFN-γ expression in mesenteric lymph nodes of ob/ob mice pre- and post-operation was detected by flow cytometry (FCM). The FXR expression in the liver and small intestine was detected by Western blotting. The mouse AML-12 liver cells were stimulated with IFN-γ at different concentrations in vitro. The changes of FXR expression were also examined. The results showed that the body weight of ob/ob mice was significantly declined from (40.6±2.7) g to (27.5±3.8) g on the 30th day after VSG (P<0.05). At the same time, VSG induced a higher level secretion of IFN-γ in mesenteric lymph nodes of ob/ob mice than that pre-operation (P<0.05). The FXR expression levels in the liver and small intestine after VSG were respectively 0.97±0.07 and 0.84±0.07 fold of GAPDH, which were significantly higher than pre-operative levels of 0.50±0.06 and 0.48±0.06 respectively (P<0.05). After the stimulation of AML-12 liver cells in vitro by different concentrations of IFN-γ (0, 10, 25, 50, 100, and 200 ng/mL), the relative FXR expression levels were 0.22±0.04, 0.31±0.04, 0.39±0.05, 0.38±0.05, 0.56±0.06, and 0.35±0.05, respectively, suggesting IFN-γ could distinctly promote the FXR expression in a dose-dependent manner in comparison to those cells without IFN-γ stimulation (P<0.05). It was concluded that VSG induces a weight loss in ob/ob mice by increasing IFN

  7. Terminal velocities of the winds from rapidly rotating OB stars

    NASA Technical Reports Server (NTRS)

    Friend, David B.

    1990-01-01

    This paper presents measurements of terminal velocities of OB stars which are rapid rotators, based on archival high-dispersion IUE spectra of the C IV resonance doublet. The terminal velocities of the most rapidly rotating stars appear to be systematically lower than those of the less rapidly rotating stars (at least for the cooler stars), although the number of very rapid rotators is only three. The modified line-radiation driven wind model of Friend and Abbott, which takes into account the finite size of the star as well as its rotation, predicts that the terminal velocity should drop with increasing rotational velocity. However, when a smaller but very homogeneous subset of the data is used (BO giants only), the correlation between terminal velocity and rotational velocity disappears.

  8. Massive star population in M 31 OB associations

    NASA Astrophysics Data System (ADS)

    Cananzi, K.

    1992-06-01

    From data taken at the 3.60 m CFHT in the U, B, V and I bands and in interference filters centered on the HeII, CIII lines and at 4749 A (continuum), we perform stellar photometry on several OB associations in the Andromeda galaxy, using DAOPHOT. This allows us to build H-R diagrams from which we deduce for V magnitudes between 16 and 22, the slope of the differential Luminosity Function (LF) and derive from it an Initial Mass Function (IMF). We used the HeII, CIII, and 'continuum' narrow bands to detect the Wolf-Rayet candidates by an 'ON LINE-OFF LINE' method. In the absence of confirmation spectra, we retain only 3 probable ones, plus 5 recently confirmed in two of our common fields by Armandroff and Massey and 2 already found by Moffat and Shara (1987), to obtain an estimation of the ratio WR/O.

  9. Quantitative spectroscopy of OB stars: from dwarfs to supergiants

    NASA Astrophysics Data System (ADS)

    Przybilla, N.; Nieva, M. F.; Firnstein, M.; Butler, K.

    2013-02-01

    We discuss recent progress made in the spectral modelling of OB stars from the main sequence to evolved phases as BA-type supergiants. Non-LTE line-formation computations can now reproduce observed spectra over the entire optical and near-IR wavelength range with high confidence, facilitating stellar atmospheric parameters and elemental abundances to be determined at high accuracy and precision. An overview is given how the fundamental stellar parameters of single stars determined in our new approach compare to high-precision data derived from detached eclipsing massive binary stars. Finally, the observational constraints for a sample of Galactic objects are put in context with state-of-the-art evolution models for rotating massive stars.

  10. New Hα stars. Cyg OB7 region. I.

    NASA Astrophysics Data System (ADS)

    Melikian, N. D.; Karapetian, A. A.

    2010-12-01

    Preliminary results from a search for emission stars in the neighborhood of Cyg OB7 are reported. A new region of size 14×14 arc min lies 12 arc min to the south of HH 448. 17 new objects have been found, of which one is related to a nebula with an interesting shape and, apparently, the characteristics of a cometary nebula. Inside the nebula there are 4 faint, compact formations with very high proper motions. The spectrum of a star shows that it is of spectral class K9-M0 and is a T Tau type star at a distance of 220 pc. Most of the discovered stars with a strong or intermediate Ha emission line intensity are presumably also T Tau stars. This region appears to be a continuation of the T association around HH 448.

  11. New Emission Stars in B Cyg OB7

    NASA Astrophysics Data System (ADS)

    Melikian, N. D.; Karapetian, A. A.; Gomez, J.

    2016-12-01

    This is a continuation of a search for and study of emission objects in known dark clouds and star formation regions. New results are presented from a search for emission stars in the region of Cyg OB7 where 36 new emission stars have been discovered previously. The current observations were made adjacent to previously studied regions in the vicinity of the object HH 448. 26 new emission stars were found in three small regions with a combined area of 0.11 sq. deg. On an (H-K)-(J-H) two-color diagram these stars lie among the classical T Tau stars (CTTS) and T Tau stars with weak lines (WTTS). A strong change in brightness was recorded for one of the emission stars.

  12. Effects of CP-900691, a novel peroxisome proliferator-activated receptor α, agonist on diabetic nephropathy in the BTBR ob/ob mouse.

    PubMed

    Askari, Bardia; Wietecha, Tomasz; Hudkins, Kelly L; Fox, Edward J; O'Brien, Kevin D; Kim, Jinkyu; Nguyen, Tri Q; Alpers, Charles E

    2014-08-01

    Piperidine-based peroxisome proliferator-activated receptor-α agonists are agents that are efficacious in improving lipid, glycemic, and inflammatory indicators in diabetes and obesity. This study sought to determine whether CP-900691 ((S)-3-[3-(1-carboxy-1-methyl-ethoxy)-phenyl]-piperidine-1-carboxylic acid 4-trifluoromethyl-benzyl ester; CP), a member of this novel class of agents, by decreasing plasma triglycerides, could prevent diabetic nephropathy in the Black and Tan, BRachyuric (BTBR) ob/ob mouse model of type 2 diabetes mellitus. Four-week old female BTBR WT and BTBR ob/ob mice received either regular chow or one containing CP (3 mg/kg per day) for 14 weeks. CP elevated plasma high-density lipoprotein, albuminuria, and urinary excretion of 8-epi PGF(2α), a product of the nonenzymatic metabolism of arachidonic acid and whose production is elevated in oxidative stress, in BTBR WT mice. In BTBR ob/ob mice, CP reduced plasma triglycerides and non-esterified fatty acids, fasting blood glucose, body weight, and plasma interleukin-6, while concomitantly improving insulin resistance. Despite these beneficial metabolic effects, CP had no effect on elevated plasma insulin, 8-epi PGF(2α) excretion, and albuminuria, and surprisingly, did not ameliorate the development of diabetic nephropathy, having no effect on the accumulation of renal macrophages, glomerular hypertrophy, and increased mesangial matrix expansion. In addition, CP did not increase plasma high-density lipoprotein in BTBR ob/ob mice, while paradoxically increasing total cholesterol levels. These findings indicate that 8-epi PGF(2α), possibly along with hyperinsulinemia and inflammatory and dysfunctional lipoproteins, is integral to the development of diabetic nephropathy and should be considered as a potential target of therapy in the treatment of diabetic nephropathy.

  13. The PPAR-γ agonist, darglitazone, restores acute inflammatory responses to cerebral hypoxia–ischemia in the diabetic ob/ob mouse

    PubMed Central

    Kumari, Rashmi; Willing, Lisa B; Patel, Shyama D; Krady, J Kyle; Zavadoski, William J; Gibbs, E Michael; Vannucci, Susan J; Simpson, Ian A

    2010-01-01

    Diabetes is an increased risk factor for stroke and results in increased brain damage in experimental animals and humans. The precise mechanisms are unclear, but our earlier studies in the db/db mice suggested that the cerebral inflammatory response initiating recovery was both delayed and diminished in the diabetic mice compared with the nondiabetic db/+ mice. In this study, we investigated the actions of the peroxisome proliferator-activated receptor (PPAR)-γ agonist darglitazone in treating diabetes and promoting recovery after a hypoxic-ischemic (H/I) insult in the diabetic ob/ob mouse. Male ob/+ and ob/ob mice received darglitazone (1 mg/kg) for 7 days before induction of H/I. Darglitazone restored euglycemia and normalized elevated corticosterone, triglycerides, and very-low-density lipoprotein levels. Darglitazone dramatically reduced the infarct size in the ob/ob mice at 24 h of recovery compared with the untreated group (30±13% to 3.3±1.6%, n=6 to 8) but did not show any significant effect in the ob/+ mice. Microglial and astrocytic activation monitored by cytokine expression (interleukin-1β and tumor necrosis factor-α) and in situ hybridization studies (bfl1 and glial fibrillary acidic protein) suggest a biphasic inflammatory response, with darglitazone restoring the compromised proinflammatory response(s) in the diabetic mouse at 4 h but suppressing subsequent inflammatory responses at 8 and 24 h in both control and diabetic mice. PMID:19861974

  14. High aminopeptidase A activity contributes to blood pressure control in ob/ob mice by AT2 receptor-dependent mechanism.

    PubMed

    Morais, Rafael L; Hilzendeger, Aline M; Visniauskas, Bruna; Todiras, Mihail; Alenina, Natalia; Mori, Marcelo A; Araújo, Ronaldo C; Nakaie, Clovis R; Chagas, Jair R; Carmona, Adriana K; Bader, Michael; Pesquero, João B

    2017-03-01

    Obesity is assumed to be a major cause of human essential hypertension; however, the mechanisms responsible for weight-related increase in blood pressure (BP) are not fully understood. The prevalence of hypertension induced by obesity has grown over the years, and the role of the renin-angiotensin-aldosterone system (RAAS) in this process continues to be elucidated. In this scenario, the ob/ob mice are a genetic obesity model generally used for metabolic disorder studies. These mice are normotensive even though they present several metabolic conditions that predispose them to hypertension. Although the normotensive trait in these mice is associated with the poor activation of sympathetic nervous system by the lack of leptin, we demonstrated that ob/ob mice present massively increased aminopeptidase A (APA) activity in the circulation. APA enzyme metabolizes angiotensin (ANG) II into ANG III, a peptide associated with intrarenal angiotensin type 2 (AT2) receptor activation and induction of natriuresis. In these mice, we found increased ANG-III levels in the circulation, high AT2 receptor expression in the kidney, and enhanced natriuresis. AT2 receptor blocking and APA inhibition increased BP, suggesting the ANG III-AT2 receptor axis as a complementary BP control mechanism. Circulating APA activity was significantly reduced by weight loss independently of leptin, indicating the role of fat tissue in APA production. Therefore, in this study we provide new data supporting the role of APA in BP control in ob/ob mouse strain. These findings improve our comprehension about obesity-related hypertension and suggest new tools for its treatment.NEW & NOTEWORTHY In this study, we reported an increased angiotensin III generation in the circulation of ob/ob mice caused by a high aminopeptidase A activity. These findings are associated with an increased natriuresis found in these mice and support the role of renin-angiotensin-aldosterone system as additional mechanism regulating

  15. Cygnus OB2 - Archaeology Of Our Closest Massive Star Factory

    NASA Astrophysics Data System (ADS)

    Van Der Veen, Erik

    2011-01-01

    Cygnus OB2 is the nearest example of a massive star-forming region, at only 1.45kpc. Despite its status and importance, we still lack a basic understanding of this complex. Practically all of its 50+ O-type stars and some of its B-type stars have been scoped, but low-mass members remain poorly studied. An extensive set of new spectra collected using the FAST and HectoSpec instruments at the Fred Lawrence Whipple Observatory is allowing for a much more detailed study of the association. Optical spectra of pre-main sequence objects, vital in developing our global understanding of star formation and the products thereof, are being analyzed in order to characterize masses and velocities of individual objects within the region. The level of reddening of the spectra is first identified, providing a more reliable estimate of spectral type than photometry alone, and from which stellar mass and temperatures are derived. Velocities are then obtained via cross-correlation and line centroiding techniques. Combining these two results will map out the distribution of velocities as a function of stellar mass. The end goal of this study is to understand the dynamics and boundedness of the cluster, and to diagnose the presence of any sub-clustering and mass segregation. In this way, Cygnus OB2 is poised to become a stepping stone with which to extend our detailed understanding of Gould Belt star forming regions down to the lowest mass stars to much more massive clusters and starbursts.

  16. Leptin administration restores the altered adipose and hepatic expression of aquaglyceroporins improving the non-alcoholic fatty liver of ob/ob mice.

    PubMed

    Rodríguez, Amaia; Moreno, Natalia R; Balaguer, Inmaculada; Méndez-Giménez, Leire; Becerril, Sara; Catalán, Victoria; Gómez-Ambrosi, Javier; Portincasa, Piero; Calamita, Giuseppe; Soveral, Graça; Malagón, María M; Frühbeck, Gema

    2015-07-10

    Glycerol is an important metabolite for the control of lipid accumulation in white adipose tissue (WAT) and liver. We aimed to investigate whether exogenous administration of leptin improves features of non-alcoholic fatty liver disease (NAFLD) in leptin-deficient ob/ob mice via the regulation of AQP3 and AQP7 (glycerol channels mediating glycerol efflux in adipocytes) and AQP9 (aquaglyceroporin facilitating glycerol influx in hepatocytes). Twelve-week-old male wild type and ob/ob mice were divided in three groups as follows: control, leptin-treated (1 mg/kg/d) and pair-fed. Leptin deficiency was associated with obesity and NAFLD exhibiting an AQP3 and AQP7 increase in WAT, without changes in hepatic AQP9. Adipose Aqp3 and hepatic Aqp9 transcripts positively correlated with markers of adiposity and hepatic steatosis. Chronic leptin administration (4-weeks) was associated with improved body weight, whole-body adiposity, and hepatosteatosis of ob/ob mice and to a down-regulation of AQP3, AQP7 in WAT and an up-regulation of hepatic AQP9. Acute leptin stimulation in vitro (4-h) induced the mobilization of aquaglyceroporins towards lipid droplets (AQP3) and the plasma membrane (AQP7) in murine adipocytes. Our results show that leptin restores the coordinated regulation of fat-specific AQP7 and liver-specific AQP9, a step which might prevent lipid overaccumulation in WAT and liver in obesity.

  17. Mogat1 deletion does not ameliorate hepatic steatosis in lipodystrophic (Agpat2−/−) or obese (ob/ob) mice

    PubMed Central

    Agarwal, Anil K.; Tunison, Katie; Dalal, Jasbir S.; Yen, Chi-Liang Eric; Farese, Robert V.; Horton, Jay D.; Garg, Abhimanyu

    2016-01-01

    Reducing triacylglycerol (TAG) in the liver continues to pose a challenge in states of nonalcoholic hepatic steatosis. Monoacylglycerol O-acyltransferase (MOGAT) enzymes convert monoacylglycerol (MAG) to diacylglycerol, a precursor for TAG synthesis, and are involved in a major pathway of TAG synthesis in selected tissues, such as small intestine. MOGAT1 possesses MGAT activity in in vitro assays, but its physiological function in TAG metabolism is unknown. Recent studies suggest a role for MOGAT1 in hepatic steatosis in lipodystrophic [1-acylglycerol-3-phosphate O-acyltransferase (Agpat)2−/−] and obese (ob/ob) mice. To test this, we deleted Mogat1 in the Agpat2−/− and ob/ob genetic background to generate Mogat1−/−;Agpat2−/− and Mogat1−/−;ob/ob double knockout (DKO) mice. Here we report that, despite the absence of Mogat1 in either DKO mouse model, we did not find any decrease in liver TAG by 16 weeks of age. Additionally, there were no measureable changes in plasma glucose (diabetes) and insulin resistance. Our data indicate a minimal role, if any, of MOGAT1 in liver TAG synthesis, and that TAG synthesis in steatosis associated with lipodystrophy and obesity is independent of MOGAT1. Our findings suggest that MOGAT1 likely has an alternative function in vivo. PMID:26880786

  18. Leptin administration restores the altered adipose and hepatic expression of aquaglyceroporins improving the non-alcoholic fatty liver of ob/ob mice

    PubMed Central

    Rodríguez, Amaia; Moreno, Natalia R.; Balaguer, Inmaculada; Méndez-Giménez, Leire; Becerril, Sara; Catalán, Victoria; Gómez-Ambrosi, Javier; Portincasa, Piero; Calamita, Giuseppe; Soveral, Graça; Malagón, María M.; Frühbeck, Gema

    2015-01-01

    Glycerol is an important metabolite for the control of lipid accumulation in white adipose tissue (WAT) and liver. We aimed to investigate whether exogenous administration of leptin improves features of non-alcoholic fatty liver disease (NAFLD) in leptin-deficient ob/ob mice via the regulation of AQP3 and AQP7 (glycerol channels mediating glycerol efflux in adipocytes) and AQP9 (aquaglyceroporin facilitating glycerol influx in hepatocytes). Twelve-week-old male wild type and ob/ob mice were divided in three groups as follows: control, leptin-treated (1 mg/kg/d) and pair-fed. Leptin deficiency was associated with obesity and NAFLD exhibiting an AQP3 and AQP7 increase in WAT, without changes in hepatic AQP9. Adipose Aqp3 and hepatic Aqp9 transcripts positively correlated with markers of adiposity and hepatic steatosis. Chronic leptin administration (4-weeks) was associated with improved body weight, whole-body adiposity, and hepatosteatosis of ob/ob mice and to a down-regulation of AQP3, AQP7 in WAT and an up-regulation of hepatic AQP9. Acute leptin stimulation in vitro (4-h) induced the mobilization of aquaglyceroporins towards lipid droplets (AQP3) and the plasma membrane (AQP7) in murine adipocytes. Our results show that leptin restores the coordinated regulation of fat-specific AQP7 and liver-specific AQP9, a step which might prevent lipid overaccumulation in WAT and liver in obesity. PMID:26159457

  19. Oral Administration of Apple Procyanidins Ameliorates Insulin Resistance via Suppression of Pro-Inflammatory Cytokine Expression in Liver of Diabetic ob/ob Mice.

    PubMed

    Ogura, Kasane; Ogura, Masahito; Shoji, Toshihiko; Sato, Yuichi; Tahara, Yumiko; Yamano, Gen; Sato, Hiroki; Sugizaki, Kazu; Fujita, Naotaka; Tatsuoka, Hisato; Usui, Ryota; Mukai, Eri; Fujimoto, Shimpei; Inagaki, Nobuya; Nagashima, Kazuaki

    2016-11-23

    Procyanidins, the main ingredient of apple polyphenols, are known to possess antioxidative and anti-inflammatory effects associated closely with the pathophysiology of insulin resistance and type 2 diabetes. We investigated the effects of orally administered apple procyanidins (APCs) on glucose metabolism using diabetic ob/ob mice. We found no difference in body weight or body composition between mice treated with APCs and untreated mice. A 4 week oral administration of APCs containing water [0.5% (w/v)] ameliorated glucose tolerance, insulin resistance, and hepatic gluconeogenesis in ob/ob mice. APCs also suppressed the increase in the level of the pancreatic β-cell. Insulin-stimulated Akt phosphorylation was significantly enhanced; pro-inflammatory cytokine expression levels were significantly decreased, and c-Jun N-terminal kinase phosphorylation was downregulated in the liver of those mice treated with APCs. In conclusion, APCs ameliorate insulin resistance by improving hepatic insulin signaling through suppression of hepatic inflammation in ob/ob mice, which may be a mechanism with possible beneficial health effects of APCs in disturbed glucose metabolism.

  20. Leptin Administration Favors Muscle Mass Accretion by Decreasing FoxO3a and Increasing PGC-1α in ob/ob Mice

    PubMed Central

    Sáinz, Neira; Rodríguez, Amaia; Catalán, Victoria; Becerril, Sara; Ramírez, Beatriz; Gómez-Ambrosi, Javier; Frühbeck, Gema

    2009-01-01

    Absence of leptin has been associated with reduced skeletal muscle mass in leptin-deficient ob/ob mice. The aim of our study was to examine the effect of leptin on the catabolic and anabolic pathways regulating muscle mass. Gastrocnemius, extensor digitorum longus and soleus muscle mass as well as fiber size were significantly lower in ob/ob mice compared to wild type littermates, being significantly increased by leptin administration (P<0.001). This effect was associated with an inactivation of the muscle atrophy-related transcription factor forkhead box class O3 (FoxO3a) (P<0.05), and with a decrease in the protein expression levels of the E3 ubiquitin-ligases muscle atrophy F-box (MAFbx) (P<0.05) and muscle RING finger 1 (MuRF1) (P<0.05). Moreover, leptin increased (P<0.01) protein expression levels of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), a regulator of muscle fiber type, and decreased (P<0.05) myostatin protein, a negative regulator of muscle growth. Leptin administration also activated (P<0.01) the regulators of cell cycle progression proliferating cell nuclear antigen (PCNA) and cyclin D1, and increased (P<0.01) myofibrillar protein troponin T. The present study provides evidence that leptin treatment may increase muscle mass of ob/ob mice by inhibiting myofibrillar protein degradation as well as enhancing muscle cell proliferation. PMID:19730740

  1. Neuroglial metabolic compartmentation underlying leptin deficiency in the obese ob/ob mice as detected by magnetic resonance imaging and spectroscopy methods

    PubMed Central

    Delgado, Teresa C; Violante, Inês R; Nieto-Charques, Laura; Cerdán, Sebastián

    2011-01-01

    Manganese-Enhanced Magnetic Resonance Imaging (MEMRI), 1H and 13C High-Resolution-Magic Angle Spinning (HR-MAS) Spectroscopy, and genomic approaches were used to compare cerebral activation and neuronal and glial oxidative metabolism in ad libitum fed C57BL6/J leptin-deficient, genetically obese ob/ob mice. T1-weighted Magnetic Resonance Images across the hypothalamic Arcuate and the Ventromedial nuclei were acquired kinetically after manganese infusion. Neuroglial compartmentation was investigated in hypothalamic biopsies after intraperitoneal injections of [1-13C]glucose or [2-13C]acetate. Total RNA was extracted to determine the effects of leptin deficiency in the expression of representative genes coding for regulatory enzymes of hypothalamic energy pathways and glutamatergic neurotransmission. Manganese-Enhanced Magnetic Resonance Imaging revealed enhanced cerebral activation in the hypothalamic Arcuate and Ventromedial nuclei of the ob/ob mice. 13C HR-MAS analysis showed increased 13C accumulation in the hypothalamic glutamate and glutamine carbons of ob/ob mice after the administration of [1-13C]glucose, a primarily neuronal substrate. Hypothalamic expression of the genes coding for glucokinase, phosphofructokinase, pyruvate dehydrogenase, and glutamine synthase was not significantly altered while pyruvate kinase expression was slightly upregulated. In conclusion, leptin deficiency associated with obesity led to increased cerebral activation in the hypothalamic Arcuate and Ventromedial nuclei, concomitant with significant increases in neuronal oxidative metabolism and glutamatergic neurotransmission. PMID:21971349

  2. DUSTY OB STARS IN THE SMALL MAGELLANIC CLOUD. I. OPTICAL SPECTROSCOPY REVEALS PREDOMINANTLY MAIN-SEQUENCE OB STARS

    SciTech Connect

    Sheets, Holly A.; Bolatto, Alberto D.; Van Loon, Jacco Th.; Oliveira, Joana M.; Sandstrom, Karin; Simon, Joshua D.; Barba, Rodolfo H.

    2013-07-10

    We present the results of optical spectroscopic follow-up of 125 candidate main sequence OB stars in the Small Magellanic Cloud (SMC) that were originally identified in the S{sup 3}MC infrared imaging survey as showing an excess of emission at 24 {mu}m indicative of warm dust, such as that associated with a transitional or debris disks. We use these long-slit spectra to investigate the origin of the 24 {mu}m emission and the nature of these stars. A possible explanation for the observed 24 {mu}m excess, that these are emission line stars with dusty excretion disks, is disproven for the majority of our sources. We find that 88 of these objects are normal stars without line emission, with spectral types mostly ranging from late-O to early-B; luminosity classes from the literature for a sub-set of our sample indicate that most are main-sequence stars. We further identify 17 emission-line stars, 7 possible emission-line stars, and 5 other objects with forbidden-line emission in our sample. We discover a new O6 Iaf star; it exhibits strong He II 4686 A emission but relatively weak N III 4640 A emission which we attribute to the lower nitrogen abundance in the SMC. Two other objects are identified with planetary nebulae, one with a young stellar object, and two with X-ray binaries. To shed additional light on the nature of the observed 24 {mu}m excess we use optical and infrared photometry to estimate the dust properties of the objects with normal O and B star spectra and compare these properties to those of a sample of hot spots in the Galactic interstellar medium (ISM). We find that the dust properties of the dusty OB star sample resemble the properties of the Galactic sample of hot spots. Some may be runaway systems with bow-shocks resulting from a large velocity difference between star and ISM. We further investigate the nature of these dusty OB stars in a companion paper presenting mid-infrared spectroscopy and additional imaging.

  3. Performance analysis of OVPN over OBS/MPLS architecture with different priorities traffic

    NASA Astrophysics Data System (ADS)

    Ji, Wei; Zhang, Min; Ye, Peida

    2005-11-01

    The implementing of optical virtual private networks (OVPNs) over an optical burst-switching (OBS) network based on MPLS is discussed. The network architecture, the core and access nodes faculty are described. We also propose a new model with three traffic priorities, which used to analysis the delay and blocking performance of OVPN over OBS/MPLS network based on queuing theory.

  4. SmedOB1 is Required for Planarian Homeostasis and Regeneration

    PubMed Central

    Yin, Shanshan; Huang, Yan; Zhangfang, Yingnan; Zhong, Xiaoqin; Li, Pengqing; Huang, Junjiu; Liu, Dan; Songyang, Zhou

    2016-01-01

    The planarian flatworm is an emerging model that is useful for studying homeostasis and regeneration due to its unique adult stem cells (ASCs). Previously, planaria were found to share mammalian TTAGGG chromosome ends and telomerases; however, their telomere protection proteins have not yet been identified. In Schmidtea mediterranea, we identified a homologue of the human protection of telomeres 1 (POT1) with an OB-fold (SmedOB1). SmedOB1 is evolutionarily conserved among species and is ubiquitously expressed throughout the whole body. Feeding with SmedOB1 double-stranded RNAs (dsRNAs) led to homeostasis abnormalities in the head and pharynx. Furthermore, several ASC progeny markers were downregulated, and regeneration was impaired. Here we found that SmedOB1 is required for telomeric DNA-protein complex formation and it associates with the telomere TTAGGG sequence in vitro. Moreover, DNA damage and apoptosis signals in planarian were significantly affected by SmedOB1 RNAi. We also confirmed these phenotypes in Dugesia japonica, another flatworm species. Our work identified a novel telomere-associated protein SmedOB1 in planarian, which is required for planarian homeostasis and regeneration. The phylogenetic and functional conservations of SmedOB1 provide one mechanism by which planarians maintain telomere and genome stability to ensure their immortality and shed light on the regeneration medicine of humans. PMID:27654173

  5. The Puppis region and the last crusade for faint OB stars

    NASA Astrophysics Data System (ADS)

    Orsatti, Ana M.

    1992-08-01

    UBV photoelectric and photographic measurements of OB stars from a list of 397 OB stars and 5 early-type supergiants and from the Luminous Stars Survey are presented. The galactic distribution of the OB stars in the region shows concentrations around the open clusters Ruprecht 44 and Ruprecht 55, and the presence of an important grouping of young stars located far below the plane. The distribution in latitude shows that young stars in the region are not restricted to a thin sheet around the plane but are spread over negative latitudes reaching at least b = -5 deg. In longitude, the OB distribution exhibits a concentration of Ob stars in the interval 244-251 deg; this is argued to be due to the presence of the local arm extension.

  6. Analysis of the kinematic structure in the Cygnus OB1 association

    NASA Astrophysics Data System (ADS)

    Costado, M. T.; Alfaro, E. J.; González, M.; Sampedro, L.

    2017-03-01

    The Cygnus OB1 association is part of a larger star-forming complex located in the direction of Cygnus, but whose main sub-systems may be distributed at different distances from the sun. We have collected radial velocity (RV) data for more than 300 stars in an area of 5 x 5 squared degrees centered in the Cygnus OB1 association from the literature. This area also covers part of the Cygnus OB3 and OB9 associations, because of the diffuse limits between them. In this poster, we present the results of a clustering analysis in the subspace of the phase space formed by angular coordinates and RV in the field of the Cygnus OB1 association using the current available data. Three main groups have been detected corresponding to different RV and distances.

  7. Selective activation of FGFR4 by an FGF19 variant does not improve glucose metabolism in ob/ob mice.

    PubMed

    Wu, Xinle; Ge, Hongfei; Lemon, Bryan; Weiszmann, Jennifer; Gupte, Jamila; Hawkins, Nessa; Li, Xiaofan; Tang, Jie; Lindberg, Richard; Li, Yang

    2009-08-25

    FGF19 is a hormone that regulates bile acid and glucose homeostasis. Progress has been made in identifying cofactors for receptor activation. However, several functions of FGF19 have not yet been fully defined, including the actions of FGF19 on target tissues, its FGF receptor specificity, and the contributions of other cofactors, such as heparin. Here, we explore the requirements for FGF19-FGFR/co-receptor interactions and signaling in detail. We show that betaKlotho was essential for FGF19 interaction with FGFRs 1c, 2c, and 3c, but FGF19 was able to interact directly with FGFR4 in the absence of betaKlotho in a heparin-dependent manner. Further, FGF19 activated FGFR4 signaling in the presence or absence of betaKlotho, but activation of FGFRs 1c, 2c, or 3c was completely betaKlotho dependent. We then generated an FGF19 molecule, FGF19dCTD, which has a deletion of the C-terminal region responsible for betaKlotho interaction. We determined that betaKlotho-dependent FGFR1c, 2c, and 3c interactions and activation were abolished, and betaKlotho-independent FGFR4 activation was preserved; therefore, FGF19dCTD is an FGFR4-specific activator. This unique FGF19 molecule specifically activated FGFR4-dependent signaling in liver and suppressed CYP7A1 expression in vivo, but was unable to activate signaling in adipose where FGFR4 expression is very low. Interestingly, unlike FGF19, treatment of ob/ob mice with FGF19dCTD failed to improve glucose levels and insulin sensitivity. These results suggest that FGF19-regulated liver bile acid metabolism could be independent of its glucose-lowering effect, and direct FGFR activation in adipose tissue may play an important role in the regulation of glucose homeostasis.

  8. Irradiated interfaces in the Ara OB1, Carina, Eagle Nebula, and Cyg OB2 massive star formation regions

    DOE PAGES

    Hartigan, P.; Palmer, J.; Cleeves, L. I.

    2012-09-05

    Regions of massive star formation offer some of the best and most easily-observed examples of radiation hydrodynamics. Boundaries where fully-ionized H II regions transition to neutral/molecular photodissociation regions (PDRs) are of particular interest because marked temperature and density contrasts across the boundaries lead to evaporative flows and fluid dynamical instabilities that can evolve into spectacular pillar-like structures. Furthermore, when detached from their parent clouds, pillars become ionized globules that often harbor one or more young stars. H2 molecules at the interface between a PDR and an H II region absorb ultraviolet light from massive stars, and the resulting fluoresced infraredmore » emission lines are an ideal way to trace this boundary independent of obscuring dust. This paper presents H2 images of four regions of massive star formation that illustrate different types of PDR boundaries. The Ara OB1 star formation region contains a striking long wall that has several wavy structures which are present in H2, but the emission is not particularly bright because the ambient UV fluxes are relatively low. In contrast, the Carina star formation region shows strong H2 fluorescence both along curved walls and at the edges of spectacular pillars that in some cases have become detached from their parent clouds. The less-spectacular but more well-known Eagle Nebula has two regions that have strong fluorescence in addition to its pillars. And while somewhat older than the other regions, Cyg OB2 has the highest number of massive stars of the regions surveyed and contains many isolated, fluoresced globules that have head–tail morphologies which point towards the sources of ionizing radiation. Our images provide a collection of potential astrophysical analogs that may relate to ablated interfaces observed in laser experiments of radiation hydrodynamics.« less

  9. Candidate X-Ray-emitting OB Stars in MYStIX Massive Star-forming Regions

    NASA Astrophysics Data System (ADS)

    Povich, Matthew S.; Busk, Heather A.; Feigelson, Eric D.; Townsley, Leisa K.; Kuhn, Michael A.

    2017-03-01

    Massive O-type and early B-type (OB) stars in the nearby Galaxy remain incompletely cataloged due to high extinction, bright visible and infrared nebular emission in H ii regions, and high field star contamination. These difficulties are alleviated by restricting the search to stars with X-ray emission. Using the X-ray point sources from the Massive Young Star-forming Complex Study in Infrared and X-Rays (MYStIX) survey of OB-dominated regions, we identify 98 MYStIX candidate OB (MOBc) stars by fitting their 1-8 μm spectral energy distributions (SEDs) with reddened stellar atmosphere models. We identify 27 additional MOBc stars based on JHK S photometry of X-ray stars lacking SED fitting. These candidate OB stars indicate that the current census of stars earlier than B1, taken across the 18 MYStIX regions studied, is less than 50% complete. We also fit the SEDs of 239 previously published OB stars to measure interstellar extinction and bolometric luminosities, revealing six candidate massive binary systems and five candidate O-type (super)giants. As expected, candidate OB stars have systematically higher extinction than previously published OB stars. Notable results for individual regions include identification of the OB population of a recently discovered massive cluster in NGC 6357, an older OB association in the M17 complex, and new massive luminous O stars near the Trifid Nebula. In several relatively poorly studied regions (RCW 38, NGC 6334, NGC 6357, Trifid, and NGC 3576), the OB populations may increase by factors of ≳ 2.

  10. The CIDA Variability Survey of Orion OB1. I. The Low-Mass Population of Ori OB1a and 1b

    NASA Astrophysics Data System (ADS)

    Briceño, Cesar; Calvet, Nuria; Hernández, J.; Vivas, A. K.; Hartmann, Lee; Downes, J. J.; Berlind, Perry

    2005-02-01

    We present results of a large-scale, multiepoch optical survey of the Orion OB1 association, carried out with the QUEST camera at the Venezuela National Astronomical Observatory. We identify for the first time the widely spread low-mass, young population in the Ori OB1a and OB1b subassociations. Candidate members were picked up by their variability in the V band and position in color-magnitude diagrams. We obtained spectra to confirm membership. In a region spanning ~68 deg2, we found 197 new young stars; of these, 56 are located in the Ori OB1a subassociation and 141 in Ori OB1b. The spatial distribution of the low-mass young stars is spatially coincident with that of the high-mass members but suggests a much sharper edge to the association. Comparison with the spatial extent of molecular gas and extinction maps indicates that the subassociation Ori OB1b is concentrated within a ringlike structure of radius ~2°(~15 pc at 440 pc), centered roughly on the star ɛ Ori in the Orion belt. The ring is apparent in 13CO and corresponds to a region with an extinction AV>=1. The stars exhibiting strong Hα emission, an indicator of active accretion, are found along this ring, whereas the center is populated with weak Hα-emitting stars. In contrast, Ori OB1a is located in a region devoid of gas and dust. We identify a grouping of stars within a ~3 deg2 area located in Ori OB1a, roughly clustered around the B2 star 25 Ori. The Herbig Ae/Be star V346 Ori is also associated with this grouping, which could be an older analog of σ Ori. Using several sets of evolutionary tracks, we find an age of 7-10 Myr for Ori OB1a and of ~4-6 Myr for Ori OB1b, consistent with previous estimates from OB stars. Indicators such as the equivalent width of Hα and near-IR excesses show that the number of accreting low-mass stars decreases sharply between Ori OB1b and Ori OB1a. These results indicate that although a substantial fraction of accreting disks remain at ages ~5 Myr, inner disks are

  11. Design, synthesis, and application of OB2C combinatorial peptide and peptidomimetic libraries.

    PubMed

    Liu, Ruiwu; Shih, Tsung-Chieh; Deng, Xiaojun; Anwar, Lara; Ahadi, Sara; Kumaresan, Pappanaicken; Lam, Kit S

    2015-01-01

    The "one-bead two-compound" (OB2C) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. An OB2C library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. When live cells are incubated with such OB2C libraries, every bead will be coated with a monolayer of cells. The cell membranes of the captured cells facing the bead surface are exposed to the library compounds tethered to each bead. A specific biochemical or cellular response can be detected with an appropriate reporter system. The OB2C method enables investigators to rapidly discover synthetic molecules that not only interact with cell-surface receptors but can also stimulate or inhibit downstream cell signaling. To demonstrate this powerful method, one OB2C peptide library and two OB2C peptidomimetic libraries were synthesized and screened against Molt-4 lymphoma cells to discover "death ligands." Apoptosis of the bead-bound cells was detected with immunocytochemistry using horseradish peroxidase (HRP)-conjugated anti-cleaved caspase-3 antibody and 3,3'-diaminobenzidine as a substrate. Two novel synthetic "death ligands" against Molt-4 cells were discovered using this OB2C library approach.

  12. Binary frequency and origin of the OB runaway stars

    SciTech Connect

    Gies, D.R.; Bolton, C.T.

    1986-06-01

    A radial velocity survey of the bright northern OB runaway stars has been undertaken to determine the frequency of binary stars in this high-velocity group. A total of 634 high-dispersion spectrograms of 36 proposed runaway stars were obtained over a two year period. Approximately half of the stars are velocity-variable; these include seven spectroscopic binaries, one possible beta-Cephei variable, and 10 stars with emission lines in their spectra. The latter group contains seven of the eight Be stars observed and three luminous O stars, and it is argued that their variability is caused by nonradial pulsation. Fifteen of the program stars have a peculiar radial velocity greater than 30 km/s; five others are probable runaways on the basis of distance from the galactic plane and proper motion. Only two of the confirmed and probable runaways, HD 3950 and HD 198846 (Y Cyg), are binaries, and both are double-lines systems. New orbital elements are presented for HD 3950 and the five new binary systems found among the low-velocity stars. The statisical methods used to assess velocity variability and periodic signals are described in detail. The higher fractional abundance of runaways among more massive stars, their binary frequency, and the properties of the runaway binaries suggest that they obtained their high velocities through single-binary, binary-binary, or n-body close encounters during the early dynamical evolution of associations. 208 references.

  13. D Recording of a 19-CENTURY OB River Ship

    NASA Astrophysics Data System (ADS)

    Pushkarev, A. A.; Zaytceva, O. V.; Vavulin, M. V.; Skorobogatova, A. Y.

    2016-06-01

    A 3D recording of a 19-century wooden ship discovered on the bank of the river Ob (Western Siberia) was performed in autumn 2015. The archaeologized ship was partly under water, partly lying ashore, buried under fluvial deposits. The 3D recording was performed in October, when the water level was at its lowest after clearing the area around the ship. A 3D recording at the place of discovery was required as part of the ship museumification and reconstruction project. The works performed were primarily aimed at preserving as much information about the object as possible. Given the location and peculiar features of the object, a combination of close-range photogrammetry and aerial photography was considered to be the best possible solution for creating a high-quality 3D model. The dismantled ship was delivered to Nizhnevartovsk Museum of Local History in October 2015. The ship is going to be reassembled using the created 3D model to be exhibited in the museum. The resulting models are also going to be used to make a virtual 3D reconstruction of the ship in the future. We shot a stereoscopic video for Nizhnevartovsk Museum of Local History to let visitors see the place of discovery and explore the ship in greater details. Besides, 3D printing allowed for creating a miniature of the ship, which is also going to be included in the exposition devoted to this unique discovery.

  14. A Photometric Survey of Ori OB1b

    NASA Astrophysics Data System (ADS)

    Merritt, Allison T.; Sherry, W.

    2011-01-01

    Several mechanisms have been suggested to describe the formation of sub-stellar mass objects (SSMOs), specifically brown dwarfs. Each proposed mechanism predicts a unique spatial distribution of the brown dwarfs relative to the O and B stars of the association. We have 9 square degrees of optical (VRI) data and 7 square degrees of NIR (JHK) data of Orion OB1b. The purpose of the survey is to obtain the photometric data that will allow us to determine the spatial distribution of brown dwarfs in this region and constrain the various formation theories. We present an overview of the survey, with an emphasis on the NIR data, as well as color-magnitude diagrams. This research was supported by the NOAO/KPNO Research Experiences for Undergraduates (REU) Program which is funded by the National Science Foundation Research Experiences for Undergraduates Program and the Department of Defense ASSURE program through Scientific Program Order No. 13 (AST-0754223) of the Cooperative Agreement No. AST-0132798 between the Association of Universities for Research in Astronomy (AURA) and the NSF.

  15. Possible detection of π^0^-decay γ-ray emission from CYG OB2 by EGRET.

    NASA Astrophysics Data System (ADS)

    Chen, W.; White, R. L.; Bertsch, D.

    1996-12-01

    We report possible detection of π^0^-decay radiation from Cyg OB2, a nearby (1.7kpc) massive OB star association. The EGRET flux (>100MeV) maps clearly indicate a point source whose error circle includes both Cyg OB2 and Cyg X-3. We show that Cyg X-3 is unlikely to be the counterpart for the EGRET source, because of the marginal spatial consistency and the lack of the 4.8-hour modulation seen in other high energy emissions from Cyg X-3. If confirmed, this will be the first detection by EGRET of massive stars.

  16. COSMIC ORIGINS SPECTROGRAPH OBSERVATIONS OF TRANSLUCENT CLOUDS: Cyg OB2 8A

    SciTech Connect

    Snow, Theodore P.; Destree, Joshua D.; Burgh, Eric B.; Ferguson, Ryan M.; Danforth, Charles W.; Cordiner, Martin E-mail: destree@colorado.ed E-mail: ryan.m.ferguson@colorado.ed E-mail: martin.cordiner@nasa.go

    2010-09-10

    Data from the Cosmic Origins Spectrograph (COS) are presented for the first highly reddened target (Cyg OB2 8A) under the COS Science Team's guaranteed time allocation. Column densities of ionic, atomic, and molecular species are reported and implications are discussed. Data from Cyg OB2 8A demonstrate the ability to analyze highly reddened interstellar sight lines with the COS that were unavailable to previous UV instruments. Measured column densities indicate that the Cyg OB2 8A line of sight contains multiple diffuse clouds rather than a dominant translucent cloud.

  17. Attenuated pain response of obese mice (B6.Cg-lep(ob)) is affected by aging and leptin but not sex.

    PubMed

    Rodgers, Helen M; Liban, Suadi; Wilson, Linda M

    2014-01-17

    Genetically obese mice (B6.Cg-lep(ob)) manifest decreased responses to noxious thermal stimuli (hotplate test) suggesting endogenous analgesia (Roy et al., 1981). To examine further the analgesic response of these mice, we conducted 4 experiments. Experiment 1 assessed the response of ob/ob mice to tail flick, another noxious thermal test. Tail-flick testing was performed on B6.Cg-lep(ob) mice (n=14) and B6.Cg-lep(OB/?) (n=12) across a range of temperatures. Ob/ob mice exhibited longer latencies than control mice at all temperatures tested. In Experiment 2, potential sex differences were examined. Tail-flick latencies in male and female ob/ob mice (n=6/group) did not differ. The final 2 experiments examined factors that could modulate endogenous analgesia. Experiment 3 assessed the effects of aging in ob/ob mice (n=10/group). Older mice displayed longer tail-flick latencies than did younger mice. Experiment 4 examined the effect of leptin administration in the leptin-deficient ob/ob mice. Two groups (n=10/group) of ob/ob mice received osmotic pump implants filled with either leptin or vehicle, and were tail-flick tested at days 7 and 14 post-implantation. Ob/ob mice receiving leptin showed shorter latencies than did vehicle-receiving ob/ob mice. Taken together, these results support earlier reports of heightened analgesia in ob/ob mice and suggest that aging further reduces the already impaired pain response. Furthermore, leptin deficiency partially contributes to decreased pain sensation of ob/ob mice. © 2013.

  18. IGFBP2 is neither sufficient nor necessary for the physiological actions of leptin on glucose homeostasis in male ob/ob mice.

    PubMed

    Neumann, Ursula H; Chen, Sam; Tam, Yuen Yi C; Baker, Robert K; Covey, Scott D; Cullis, Pieter R; Kieffer, Timothy J

    2014-03-01

    The ability of leptin to improve metabolic abnormalities in models of leptin deficiency, lipodystrophy, and even type 1 diabetes is of significant interest. However, the mechanism by which leptin mediates these effects remains ill-defined. Leptin was recently reported to regulate insulin-like growth factor-binding protein-2 (IGFBP2), and adenoviral overexpression of pharmacological levels of IGFBP2 ameliorates diabetic symptoms in many models of diabetes. We sought to determine the role of physiological levels of IGFBP2 in the glucoregulatory action of leptin. To investigate whether physiological levels of IGFBP2 are sufficient to mimic the action of leptin, we treated male ob/ob mice with low-dose IGFBP2 adenovirus (Ad-IGFBP2) or low-dose leptin. Despite similar levels of circulating IGFBP2, leptin but not Ad-IGFBP2 lowered body weight and plasma insulin and improved glucose and insulin tolerance. To elucidate the role of IGFBP2 in normal glucose homeostasis, we knocked down IGFBP2 in male C57BL/6 mice using small interfering RNA to determine whether this would recapitulate any aspect of the ob/ob phenotype. Despite successful IGFBP2 knockdown, body weight, blood glucose, and plasma insulin were unchanged. Finally, to determine whether IGFBP2 is required for the glucoregulatory actions of leptin, we prevented leptin-mediated increases in IGFBP2 in male ob/ob mice using RNA interference. Even though increases in IGFBP2 were blocked, the ability of leptin to decrease body weight, blood glucose, and plasma insulin levels were unaltered. In conclusion, physiological levels of IGFBP2 are neither sufficient to mimic nor required for the physiological action of leptin.

  19. Impaired contractile responses and altered expression and phosphorylation of Ca2+ sensitization proteins in gastric antrum smooth muscles from ob/ob mice

    PubMed Central

    Bhetwal, Bhupal P.; An, Changlong; Baker, Salah A.; Lyon, Kristin L.

    2013-01-01

    Diabetic gastroparesis is a common complication of diabetes, adversely affecting quality of life with symptoms of abdominal discomfort, nausea, and vomiting. The pathogenesis of this complex disorder is not well understood, involving abnormalities in the extrinsic and enteric nervous systems, interstitial cells of Cajal (ICCs), smooth muscles and immune cells. The ob/ob mouse model of obesity and diabetes develops delayed gastric emptying, providing an animal model for investigating how gastric smooth muscle dysfunction contributes to the pathophysiology of diabetic gastroparesis. Although ROCK2, MYPT1, and CPI-17 activities are reduced in intestinal motility disorders, their functioning has not been investigated in diabetic gastroparesis. We hypothesized that reduced expression and phosphorylation of the myosin light chain phosphatase (MLCP) inhibitory proteins MYPT1 and CPI-17 in ob/ob gastric antrum smooth muscles could contribute to the impaired antrum smooth muscle function of diabetic gastroparesis. Spontaneous and carbachol- and high K+-evoked contractions of gastric antrum smooth muscles from 7 to 12 week old male ob/ob mice were reduced compared to age- and strain-matched controls. There were no differences in spontaneous and agonist-evoked intracellular Ca2+ transients and myosin light chain kinase expression. The F-actin:G-actin ratios were similar. Rho kinase 2 (ROCK2) expression was decreased at both ages. Basal and agonist-evoked MYPT1 and myosin light chain 20 phosphorylation, but not CPI-17 phosphorylation, was reduced compared to age-matched controls. These findings suggest that reduced MLCP inhibition due to decreased ROCK2 phosphorylation of MYPT1 in gastric antrum smooth muscles contributes to the antral dysmotility of diabetic gastroparesis. PMID:23576331

  20. Yhhu981, a novel compound, stimulates fatty acid oxidation via the activation of AMPK and ameliorates lipid metabolism disorder in ob/ob mice

    PubMed Central

    Zeng, Hong-liang; Huang, Su-ling; Xie, Fu-chun; Zeng, Li-min; Hu, You-hong; Leng, Ying

    2015-01-01

    Aim: Defects in fatty acid metabolism contribute to the pathogenesis of insulin resistance and obesity. In this study, we investigated the effects of a novel compound yhhu981 on fatty acid metabolism in vitro and in vivo. Methods: The capacity to stimulate fatty acid oxidation was assessed in C2C12 myotubes. The fatty acid synthesis was studied in HepG2 cells using isotope tracing. The phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) was examined with Western blot analysis. For in vivo experiments, ob/ob mice were orally treated with yhhu981 acutely (300 mg/kg) or chronically (150 or 300 mg·kg−1·d−1 for 22 d). On the last day of treatment, serum and tissue samples were collected for analysis. Results: Yhhu981 (12.5–25 μmol/L) significantly increased fatty acid oxidation and the expression of related genes (Sirt1, Pgc1α and Mcad) in C2C12 myotubes, and inhibited fatty acid synthesis in HepG2 cells. Furthermore, yhhu981 dose-dependently increased the phosphorylation of AMPK and ACC in both C2C12 myotubes and HepG2 cells. Compound C, an AMPK inhibitor, blocked fatty acid oxidation in yhhu981-treated C2C12 myotubes and fatty acid synthesis decrease in yhhu981-treated HepG2 cells. Acute administration of yhhu981 decreased the respiratory exchange ratio in ob/ob mice, whereas chronic treatment with yhhu981 ameliorated the lipid abnormalities and ectopic lipid deposition in skeletal muscle and liver of ob/ob mice. Conclusion: Yhhu981 is a potent compound that stimulates fatty acid oxidation, and exerts pleiotropic effects on lipid metabolism by activating AMPK. PMID:25732571

  1. Study of eclipsing binary and multiple systems in ob associations. II. The cygnus ob region: V443 Cyg, V456 Cyg, and V2107 Cyg

    SciTech Connect

    Bakış, V.; Bakış, H.; Hensberge, H.; Bilir, S.; Yılmaz, F.; Kıran, E.; Demircan, O.; Zejda, M.; Mikulášek, Z.

    2014-06-01

    Three presumably young eclipsing binary systems in the direction of the Cygnus OB1, OB3, and OB9 associations are studied. Component spectra are reconstructed and their orbits are determined using light curves and spectra disentangling techniques. V443 Cyg and V456 Cyg have circular orbits while the light curve of V2107 Cyg imposes a slightly eccentric orbit (e = 0.045 ± 0.03). V443 Cyg harbors F-type stars, not young early-A stars as previously suggested in the literature based solely on photometry. It appears to be situated in the foreground (distance 0.6 ± 0.2 kpc) of the young stellar populations in Cygnus. V456 Cyg, at a distance of 0.50 ± 0.03 kpc, consists of a slightly metal-weak A-type star and an early-F star. The age of both systems, on or very near to the main sequence, remains uncertain by an order of magnitude. V2107 Cyg is a more massive system (8.9 ± 2 and 4.5 ± 1.2 M {sub ☉}) at 1.5 ± 0.5 kpc and, also kinematically, a strong candidate-member of Cyg OB1. The more massive component is slightly evolved and appears to undergo non-radial βCep-type pulsations. The Doppler signal of the secondary is barely detectable. A more extensive, asteroseismological study is necessary to fix masses more precisely. Nevertheless, the position of the primary in the H-R diagram confines the age reasonably well to 20 ± 5 Myr, indicating that for Cyg OB1 has a similar extent of star formation history as that established for Cyg OB2.

  2. Divergent effects of a CLA-enriched beef diet on metabolic health in ApoE-/- and ob/ob mice.

    PubMed

    Reynolds, Clare M; Toomey, Sinead; McBride, Rachael; McMonagle, Jolene; Morine, Melissa J; Belton, Orina; Moloney, Aidan P; Roche, Helen M

    2013-02-01

    Conjugated linoleic acid (CLA) is found naturally in meat and dairy products, and represents a potential therapeutic functional nutrient. However, given the discrepancies in isomer composition and concentration, controversy surrounds its proposed antidiabetic, antiobesity effects. This study focused on the effects of CLA-enriched beef (composed predominantly of c9, t11-CLA) in two separate models of metabolic disease: proatherosclerotic ApoE(-/-) mice and diabetic, leptin-deficient ob/ob mice. Animals were fed CLA-enriched beef for 28 days, and markers of the metabolic syndrome and atherosclerosis were assessed. Comprehensive hepatic transcriptomic analysis was completed to understand divergent metabolic effects of CLA. CLA-enriched beef significantly reduced plasma glucose, insulin, nonesterified fatty acid and triacylglycerol and increased adiponectin levels in ob/ob mice. In contrast, plasma lipid profiles and glucose homeostasis deteriorated and promoted atherosclerosis following the CLA-enriched beef diet in ApoE(-/-) mice. Hepatic transcriptomic profiling revealed divergent effects of CLA-enriched beef on insulin signaling and lipogenic pathways, which were adversely affected in ApoE(-/-) mice. This study demonstrated clear divergence in the effects of CLA. CLA-enriched beef improved metabolic flexibility in ob/ob mice, resulting in enhanced insulin sensitivity. However, CLA-enriched diet increased expression of lipogenic genes, resulting in inefficient fatty acid storage which increases lipotoxicity in peripheral organs, and led to profound metabolic dysfunction in ApoE(-/-) mice. While CLA may have potential health effects, in some circumstances, caution must be exercised in presenting this bioactive lipid as a potential functional food for the treatment of metabolic disease.

  3. Arctigenin, a natural compound, activates AMP-activated protein kinase via inhibition of mitochondria complex I and ameliorates metabolic disorders in ob/ob mice.

    PubMed

    Huang, S-L; Yu, R-T; Gong, J; Feng, Y; Dai, Y-L; Hu, F; Hu, Y-H; Tao, Y-D; Leng, Y

    2012-05-01

    Arctigenin is a natural compound that had never been previously demonstrated to have a glucose-lowering effect. Here it was found to activate AMP-activated protein kinase (AMPK), and the mechanism by which this occurred, as well as the effects on glucose and lipid metabolism were investigated. 2-Deoxyglucose uptake and AMPK phosphorylation were examined in L6 myotubes and isolated skeletal muscle. Gluconeogenesis and lipid synthesis were evaluated in rat primary hepatocytes. The acute and chronic effects of arctigenin on metabolic abnormalities were observed in C57BL/6J and ob/ob mice. Changes in mitochondrial membrane potential were measured using the J-aggregate-forming dye, JC-1. Analysis of respiration of L6 myotubes or isolated mitochondria was conducted in a channel oxygen system. Arctigenin increased AMPK phosphorylation and stimulated glucose uptake in L6 myotubes and isolated skeletal muscles. In primary hepatocytes, it decreased gluconeogenesis and lipid synthesis. The enhancement of glucose uptake and suppression of hepatic gluconeogenesis and lipid synthesis by arctigenin were prevented by blockade of AMPK activation. The respiration of L6 myotubes or isolated mitochondria was inhibited by arctigenin with a specific effect on respiratory complex I. A single oral dose of arctigenin reduced gluconeogenesis in C57BL/6J mice. Chronic oral administration of arctigenin lowered blood glucose and improved lipid metabolism in ob/ob mice. This study demonstrates a new role for arctigenin as a potent indirect activator of AMPK via inhibition of respiratory complex I, with beneficial effects on metabolic disorders in ob/ob mice. This highlights the potential value of arctigenin as a possible treatment of type 2 diabetes.

  4. Anti-obesity effects of KR-66195, a synthetic DPP-IV inhibitor, in diet-induced obese mice and obese-diabetic ob/ob mice.

    PubMed

    Lee, Eun Young; Kim, Yeon Wook; Oh, Hyunhee; Choi, Cheol Soo; Ahn, Jin Hee; Lee, Byung-Wan; Kang, Eun Seok; Cha, Bong Soo; Lee, Hyun Chul

    2014-06-01

    We investigated whether KR-66195, a new synthetic dipeptidyl dipeptidase IV inhibitor, could prevent weight gain, as well as improving glycemic control in diet-induced obese (DIO) and ob/ob mice. Male C57BL/6 mice were randomly assigned to the following groups: chow diet, high-fat diet, and high-fat diet with KR-66195. After KR-66195 treatment for eight weeks, intraperitoneal glucose tolerance tests were performed. A pair-feeding study was performed to investigate the mechanisms of the anti-obesity effects of KR-66195 in DIO mice. Female ob/ob mice were treated with KR-66195 for three weeks and compared to the vehicle-treated group. In DIO mice, KR-66195 treatment increased the plasma glucagon-like peptide (GLP)-1 levels and improved glucose tolerance. This treatment also reduced body weight gain (5.38±0.94 g vs. 12.08±0.55 g, P<0.01) and food intake (2.41±0.09 g vs. 2.79±0.11 g, P<0.05). In ob/ob mice, KR-66195 treatment for three weeks resulted in comparable effects in DIO mice. In the pair-feeding study, KR-66195-treated mice exhibited a 16% increase in energy expenditure (kcal/h/kg lean body mass) without significant differences in body weight or activities compared with pair-fed mice. These results suggest that KR-66195 prevented weight gain in DIO mice by decreasing food intake, as well as increasing energy expenditure. KR-66195 markedly increased plasma levels of GLP-1, resulting in the probable improvement in glucose tolerance and reduced body weight and food intake. Thus, KR-66195 might be further developed as a therapeutic drug to treat obesity, as well as diabetes. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Post-T Tauri Stars in the Nearest OB Association

    NASA Astrophysics Data System (ADS)

    Mamajek, Eric E.; Meyer, Michael R.; Liebert, James

    2002-09-01

    We present results of a spectroscopic survey of X-ray- and proper-motion-selected samples of late-type stars in the Lower Centaurus-Crux (LCC) and Upper Centaurus-Lupus (UCL) subgroups of the nearest OB association: Scorpius-Centaurus. The primary goals of the survey are to determine the star formation history of the OB subgroups and to assess the frequency of accreting stars in a sample dominated by ``post-T Tauri'' pre-main-sequence (PMS) stars. We investigate two samples: (1) proper-motion candidates from the ACT Catalog and Tycho Reference Catalog (TRC) with X-ray counterparts in the ROSAT All-Sky Survey (RASS) Bright Source Catalog and (2) G- and K-type stars in the Hipparcos catalog found to be candidate members by de Zeeuw et al. We obtained optical spectra of 130 candidates with the Siding Spring 2.3 m dual-beam spectrograph. PMS stars were identified by (1) strong Li λ6707 absorption, (2) subgiant surface gravities, (3) proper motions consistent with Sco-Cen membership, and (4) H-R diagram positions consistent with being PMS. We find 93% of the RASS-ACT/TRC stars to be probable PMS members, compared with 73% of the Hipparcos candidates. We demonstrate that measuring the gravity-sensitive band ratio of Sr II λ4077 to Fe I λ4071 is a valuable means of discriminating PMS and zero-age main-sequence (ZAMS) stars. Using secular parallaxes and Hipparcos, Tycho-2, and Two Micron All Sky Survey photometry, we construct an H-R diagram. Depending on the choice of published evolutionary tracks, we find the mean ages of the PMS populations to range between 17 and 23 Myr for LCC and 15 and 22 Myr for UCL. Taking into account observational errors, it appears that 95% of the low-mass star formation in each subgroup must have occurred in less than 8 Myr (LCC) and 12 Myr (UCL). Using the Bertelli et al. tracks, we find main-sequence turnoff ages for Hipparcos B-type members to be 16+/-1 Myr for LCC and 17+/-1 Myr for UCL. Contrary to previous findings, it appears that

  6. Tugboat model for OB binaries, X-ray stars and pulsars.

    PubMed

    Helfand, D J; Tademaru, E

    1977-05-12

    An examination of the kinematical properties of binary OB stars, binary X-ray sources and pulsars suggests an evolutionary sequence linking an apparent low-velocity class of pulsars to the binary nature of their extreme Population I progenitors.

  7. Mass loss from very luminous OB stars and the Cygnus superbubble

    NASA Astrophysics Data System (ADS)

    Abbott, D. C.; Bieging, J. H.; Churchwell, E.

    1981-11-01

    A description is presented of radio continuum measurements of mass loss from OB stars made at a wavelength of 6 cm with the Very Large Array (VLA). Five of the six most luminous members of the stellar association Cyg OB2 were detected. The considered investigation concentrates on the most luminous stars known in the Galaxy. The stars were selected because they would exhibit the most extreme consequences of mass loss and because they tend to be the brightest radio sources. The new mass loss rates are used to predict the properties of the interstellar bubble produced by the stellar winds of Cyg OB2. The results support the interpretation of Cash et al. (1980) that X-ray emission in the direction of Cygnus indicates a superbubble whose hot interior cavity contains the Cyg OB2 association.

  8. Determination of OB/OD/SF Emission Factors using Unmanned Aerial Systems

    EPA Science Inventory

    Instrumented, unmanned aerial systems (UASs) have been used successfully in eight campaigns since 2010 to determine emission factors from open burning (OB), open detonation (OD), and static firing (SF) demilitarization activities. These systems have sampled directly from the plu...

  9. Spectroscopic Confirmation of the Dragonfish Association: The Galaxy's Most Luminous OB Association

    NASA Astrophysics Data System (ADS)

    Rahman, Mubdi; Moon, Dae-Sik; Matzner, Christopher D.

    2011-12-01

    Young OB associations with masses greater than 104 M ⊙ have been inferred to exist in the Galaxy but have largely evaded detection. Recently, a candidate OB association has been identified within the most luminous star-forming complex in the Galaxy, the Dragonfish Nebula. We identify 18 young, massive stars with near-infrared spectroscopy from a sample of 50 members within the candidate OB association, including 15 O-type and 3 luminous blue variables or Wolf-Rayet stars. This number matches the expected yield of massive stars from the candidate association, confirming its existence and ability to power the parent star-forming complex. These results demonstrate the existence of a 105 M ⊙ OB association, more powerful than any previously known in the Galaxy, comparable in mass only to Westerlund 1. Further, the results also validate the color selection method used to identify the association, adding credence to others discovered in the same way.

  10. DEBRIS DISKS IN THE UPPER SCORPIUS OB ASSOCIATION

    SciTech Connect

    Carpenter, John M.; Hillenbrand, Lynne A.; Mamajek, Eric E.; Meyer, Michael R.

    2009-11-10

    We present MIPS 24 mum and 70 mum photometry for 205 members of the Upper Scorpius OB Association. These data are combined with published MIPS photometry for 15 additional association members to assess the frequency of circumstellar disks around 5 Myr old stars with spectral types between B0 and M5. Twelve stars have a detectable 70 mum excess, each of which also has a detectable 24 mum excess. A total of 54 stars are identified with a 24 mum excess more than 32% above the stellar photosphere. The MIPS observations reveal 19 excess sources-8 A/F/G stars and 11 K/M stars-that were not previously identified with an 8 mum or 16 mum excess. The lack of short-wavelength emission and the weak 24 mum excess suggests that these sources are debris systems or the remnants of optically thick primordial disks with inner holes. Despite the wide range of luminosities of the stars hosting apparent debris systems, the excess characteristics are consistent with all stars having dust at similar orbital radii after factoring in variations in the radiation blowout particle size with spectral type. The results for Upper Sco are compared to similar photometric surveys from the literature to re-evaluate the evolution of debris emission. After considering the completeness limits of published surveys and the effects of stellar evolution on the debris luminosity, we find that the magnitude of the 24 mum excess around F-type stars increases between ages of 5 and 17 Myr as found by previous studies, but at approx<2.6sigma confidence. For B7-A9 and G0-K5 stars, any variations in the observed 24 mum excess emission over this age range are significant at less than 2sigma confidence.

  11. Comparative Precise Parameters for OB Stars in Three Galaxies

    NASA Astrophysics Data System (ADS)

    Walborn, Nolan

    2014-10-01

    The chemical abundances, wind terminal velocities, and mass-loss rates of OB stars in the Small and Large Magellanic Clouds will be determined homogeneously from high-resolution spectroscopic data in the Mikulski Archive; and they will be further compared with analogous determinations in the Solar Neighborhood. As is well known, the three systems offer a metallicity sequence with values in solar units generally given as 0.2, 0.5, and 1, respectively, which should have corresponding effects on the metallic-line-driven winds. However, the quantitative basis for that general result can and should be improved for various reasons. For instance, it is based on heterogeneous analyses, some dated, of data with varying quality. Moreover, there is not a single metallicity but different relative values for different elements, seldom available for individual stars, with CNO significantly affected by internal evolutionary processes. We propose advances with state-of-the-art analyses of the best data, primarily from STIS and COS in the UV, but also incorporating FUSE observations of the same stars, and IUE high-resolution of a few. We shall also analyze correlative groundbased optical data. J-CB and collaborators have already published recent results for Galactic supergiants and SMC dwarfs, while work on the SMC giants/supergiants is in progress. We shall build upon that work with further Galactic and SMC data, and especially with the still relatively small but significant LMC UV sample, with detailed spectral-type matching insofar as possible. We shall also produce an atlas of all spectra analyzed, to be placed in the Archive as a high-level product to guide future work.

  12. Variability survey of brightest stars in selected OB associations

    NASA Astrophysics Data System (ADS)

    Laur, Jaan; Kolka, Indrek; Eenmäe, Tõnis; Tuvikene, Taavi; Leedjärv, Laurits

    2017-02-01

    Context. The stellar evolution theory of massive stars remains uncalibrated with high-precision photometric observational data mainly due to a small number of luminous stars that are monitored from space. Automated all-sky surveys have revealed numerous variable stars but most of the luminous stars are often overexposed. Targeted campaigns can improve the time base of photometric data for those objects. Aims: The aim of this investigation is to study the variability of luminous stars at different timescales in young open clusters and OB associations. Methods: We monitored 22 open clusters and associations from 2011 to 2013 using a 0.25-m telescope. Variable stars were detected by comparing the overall light-curve scatter with measurement uncertainties. Variability was analysed by the light curve feature extraction tool FATS. Periods of pulsating stars were determined using the discrete Fourier transform code SigSpec. We then classified the variable stars based on their pulsation periods and available spectral information. Results: We obtained light curves for more than 20 000 sources of which 354 were found to be variable. Amongst them we find 80 eclipsing binaries, 31 α Cyg, 13 β Cep, 62 Be, 16 slowly pulsating B, 7 Cepheid, 1 γ Doradus, 3 Wolf-Rayet and 63 late-type variable stars. Up to 55% of these stars are potential new discoveries as they are not present in the Variable Star Index (VSX) database. We find the cluster membership fraction for variable stars to be 13% with an upper limit of 35%. Variable star catalogue (Tables A.1-A.10) and light curves are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/598/A108

  13. Abortion training to be required in standard Ob / Gyn curriculum.

    PubMed

    1995-02-24

    On February 15, (1995) the Accreditation Council for Graduate Medical Education announced that it will now require medical schools seeking accreditation to provide abortion training for all residents in obstetrics and gynecology. The new "Program Requirements for Residency Education in Obstetrics and Gynecology," approved unanimously, will take effect on January 1, 1996. According to the Council, the newly issued standards are the first to refer specifically to abortion. The language states, "Experience with induced abortion must be part of residency training, except for residents with moral or religious objections .... Experience with management of complications of abortion must be provided to all residents." The Council also mandates that if a medical school itself has "a religious, moral or legal" objection to teaching the procedure, it must "ensure that residents ... who do not have a religious or moral objection receive education and experience in performing abortion at another institution." Other revisions provide for expanded resident education in "primary and preventive care," due to the fact that many women rely on their obstetricians and gynecologists as their primary care physicians, as well as additional training experience in family planning, including "all reversible methods of contraception" and sterilization. In order to be certified by the American Board of Obstetrics and Gynecology, ob/gyns must graduate from an accredited residency program. In addition, teaching hospitals must be accredited to secure federal reimbursements for the medical services patients receive from residents. The Accreditation Council for Graduate Medical Education operates under the aegis of the American Medical Association, the American Board of Medical Specialties, the American Hospital Association, the Association of American Medical Colleges, and the Council of Medical Specialty Societies. Both the American Board of Obstetrics and Gynecology and the American College of

  14. ObsPack: a framework for the preparation, delivery, and attribution of atmospheric greenhouse gas data

    NASA Astrophysics Data System (ADS)

    Masarie, K. A.; Peters, W.; Jacobson, A. R.; Tans, P. P.

    2014-09-01

    Observation Package (ObsPack) is a framework designed to bring together atmospheric greenhouse gas observations from a variety of sampling platforms, prepare them with specific applications in mind, and package and distribute them in a self-consistent and well-documented product. Data products created using the ObsPack framework (called "ObsPack products") are intended to support carbon cycle modeling studies and represent the next generation of value-added greenhouse gas observation products modeled after the cooperative GLOBALVIEW products introduced in 1996. Depending on intended use, ObsPack products may include data in their original form reformatted using the ObsPack framework or may contain derived data consisting of averages, subsets or smoothed representations of original data. All products include extensive ancillary information (metadata) intended to help ensure the data are used appropriately, their calibration and quality assurance history are clearly described, and that individuals responsible for the measurements (data providers or principal investigators (PIs)) are properly acknowledged for their work. ObsPack products are made freely available using a distribution strategy designed to improve communication between data providers and product users. The strategy includes a data usage policy that requires users to directly communicate with data providers and an automated e-mail notification system triggered when a product is accessed. ObsPack products will be assigned a unique Digital Object Identifier (DOI) to ensure each product can be unambiguously identified in scientific literature. Here we describe the ObsPack framework and its potential role in supporting the evolving needs of both data providers and product users.

  15. Intrinsic ultraviolet (912-3200 A) energy distribution of OB stars

    NASA Technical Reports Server (NTRS)

    Longo, Renata; Stalio, Roberto; Polidan, Ronald S.; Rossi, Lucio

    1989-01-01

    Low-dispersion 500-3200-A spectra of 20 OB stars are used to derive three individual extinction curves in the wavelength range 912-1200 A. The dependence of the intrinsic UV fluxes of OB stars on both spectral type and luminosity is demonstrated. UV color indices are proposed. The results confirm the previous finding that O star spectra are not significantly bluer than the spectrum of the B0 main-sequence star Upsilon Ori.

  16. ObsPack: a framework for the preparation, delivery, and attribution of atmospheric greenhouse gas measurements

    NASA Astrophysics Data System (ADS)

    Masarie, K. A.; Peters, W.; Jacobson, A. R.; Tans, P. P.

    2014-12-01

    Observation Package (ObsPack) is a framework designed to bring together atmospheric greenhouse gas observations from a variety of sampling platforms, prepare them with specific applications in mind, and package and distribute them in a self-consistent and well-documented product. Data products created using the ObsPack framework (called "ObsPack products") are intended to support carbon cycle modeling studies and represent a next generation of value-added greenhouse gas observation products modeled after the cooperative GLOBALVIEW products introduced in 1996. Depending on intended use, ObsPack products may include data in their original form reformatted using the ObsPack framework or may contain derived data consisting of averages, subsets, or smoothed representations of original data. All products include extensive ancillary information (metadata) intended to help ensure the data are used appropriately, their calibration and quality assurance history are clearly described, and that individuals responsible for the measurements (data providers or principal investigators (PIs)) are properly acknowledged for their work. ObsPack products are made freely available using a distribution strategy designed to improve communication between data providers and product users. The strategy includes a data usage policy that requires users to directly communicate with data providers and an automated e-mail notification system triggered when a product is accessed. ObsPack products will be assigned a unique digital object identifier (DOI) to ensure each product can be unambiguously identified in scientific literature. Here we describe the ObsPack framework and its potential role in supporting the evolving needs of both data providers and product users.

  17. A Catalog of New Spectroscopically Confirmed Massive OB Stars in Carina

    NASA Astrophysics Data System (ADS)

    Alexander, Michael J.; Hanes, Richard J.; Povich, Matthew S.; McSwain, M. Virginia

    2016-12-01

    The Carina star-forming region is one of the largest in the Galaxy, and its massive star population is still being unveiled. The large number of stars combined with high, and highly variable, interstellar extinction makes it inherently difficult to find OB stars in this type of young region. We present the results of a spectroscopic campaign to study the massive star population of the Carina Nebula, with the primary goal to confirm or reject previously identified Carina OB star candidates. A total of 141 known O- and B-type stars and 94 candidates were observed, of which 73 candidates had high enough signal-to-noise ratio to classify. We find 23 new OB stars within the Carina Nebula, a 32% confirmation rate. One of the new OB stars has blended spectra and is suspected to be a double-lined spectroscopic binary (SB2). We also reclassify the spectral types of the known OB stars and discover nine new SB2s among this population. Finally, we discuss the spatial distribution of these new OB stars relative to known structures in the Carina Nebula.

  18. Novel leptin OB3 peptide-induced signaling and progression in thyroid cancers: Comparison with leptin

    PubMed Central

    Hsieh, Meng-Ti; Lai, Hsuan-Yu; Ke, Chien-Chih; Crawford, Dana R.; Lee, Oscar K.; Fu, Earl; Mousa, Shaker A.; Grasso, Patricia; Liu, Leroy F.; Chang, Heng-Yu; Tang, Heng-Yuan; Lin, Hung-Yun; Davis, Paul J.

    2016-01-01

    Obesity results in increased secretion of cytokines from adipose tissue and is a risk factor for various cancers. Leptin is largely produced by adipose tissue and cancer cells. It induces cell proliferation and may serve to induce various cancers. OB3-leptin peptide (OB3) is a new class of functional leptin peptide. However, its mitogenic effect has not been determined. In the present study, because of a close link between leptin and the hypothalamic-pituitary-thyroid axis, OB3 was compared with leptin in different thyroid cancer cells for gene expression, proliferation and invasion. Neither agent stimulated cell proliferation. Leptin stimulated cell invasion, but reduced adhesion in anaplastic thyroid cancer cells. Activated ERK1/2 and STAT3 contributed to leptin-induced invasion. In contrast, OB3 did not affect expression of genes involved in proliferation and invasion. In vivo studies in the mouse showed that leptin, but not OB3, significantly increased circulating levels of thyrotropin (TSH), a growth factor for thyroid cancer. In summary, OB3 is a derivative of leptin that importantly lacks the mitogenic effects of leptin on thyroid cancer cells. PMID:27050378

  19. Ablation of Ghrelin O-Acyltransferase Does Not Improve Glucose Intolerance or Body Adiposity in Mice on a Leptin-Deficient ob/ob Background

    PubMed Central

    Kirchner, Henriette; Heppner, Kristy M.; Holland, Jenna; Kabra, Dhiraj; Tschöp, Matthias H.; Pfluger, Paul T.

    2013-01-01

    Type 2 Diabetes is a global health burden and based on current estimates will become an even larger problem in the future. Developing new strategies to prevent and treat diabetes is a scientific challenge of high priority. The stomach hormone ghrelin has been associated with playing a role in the regulation of glucose homeostasis. However, its precise mechanism and impact on whole glucose metabolism remains to be elucidated. This study aims to clarify the role of the two ghrelin isoforms acyl- and desacyl ghrelin in regulating glucose homeostasis. Therefore ghrelin activating enzyme Ghrelin-O-acyltransferase (GOAT) was ablated in leptin-deficient ob/ob mice to study whether specific acyl ghrelin deficiency or desacyl ghrelin abundance modifies glucose tolerance on a massively obese background. As targeted deletion of acyl ghrelin does not improve glucose homeostasis in our GOAT-ob/ob mouse model we conclude that neither acyl ghrelin nor the increased ratio of desacyl/acyl ghrelin is crucial for controlling glucose homeostasis in the here presented model of massive obesity induced by leptin deficiency. PMID:23630616

  20. Pycnogenol supplementation promotes lipolysis via activation of cAMP-dependent PKA in ob/ob mice and primary-cultured adipocytes.

    PubMed

    Ho, Jin-Nyoung; Kim, Ok-Kyung; Nam, Da-Eun; Jun, Woojin; Lee, Jeongmin

    2014-01-01

    This study investigated the PKA-dependent inhibitory effect of pycnogenol (Pyc) on lipolysis using ob/ob mice and primary mouse adipocytes. Supplementation of Pyc at 30 mg/kg significantly reduced body weight gain and visceral fat mass. The serum and hepatic triglyceride (TG) and total cholesterol (TC) levels were reduced by Pyc supplementation, and high density lipoprotein (HDL)-cholesterol level significantly increased. In addition, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA levels increased with Pyc supplementation in adipose tissue of ob/ob mice. The treatment of primary cultured adipocytes with Pyc at 100 μg/mL significantly increased glycerol release, cAMP level by reduction of phosphodiestersae-3B (PDE3B), and HSL levels, but decreased protein levels of perilipin A and fatty acid synthetase (FAS). The PKA inhibitor (H89) clearly blocked the cellular levels of perilipin A and HSL, suggesting that Pyc promotes lipolysis of adipocytes through activation of cAMP-dependent PKA, resulting in induction of HSL and reduction of perilipin A. Therefore, this study may elucidate the possible mechanism of Pyc, which is a candidate for weight loss through stimulation of lipolysis.

  1. Detached binary system MQ Centauri and its relation with Crux OB1 Association

    NASA Astrophysics Data System (ADS)

    Tunc, Efecan; Bakış, Volkan

    2016-07-01

    In this study, the astrophysical characteristics of eclipsing binary system MQ Cen and its relation with Crux OB1 association has been investigated by means of analysing high resolution (R ˜48000) spectra and light curves in Strömgren bands. Analysis of the available data yielded high precision parameters of the system which allowed us to derive reliable age and distance. The Fourier disentangling method was applied to the composite spectra of MQ Cen for reconstructing the component spectra. The modeling of the high resolution spectra revealed that the secondary component of MQ Cen rotates slower than the synchronous rotation velocity unlike the primary star which is in synchronous rotation. The photometric distance of the binary was found to be consistent with the distance given for Crux OB1 associaton (2.7 kpc) by Kaltcheva and Georgiev (1993). Space velocity of the system also supports the membership of MQ Cen to the Crux OB1 association. Age estimation was made by comparing the positions of components on HR diagram with isochrones. Conclusion shows that MQ Cen is older than the age determined for Crux OB1 association in the literature. This result implies that MQ Cen may belong to one of the older subgroups in the Crux OB1 association complex and the stellar formation in the complex started long ago than previously predicted. The evolutionary status of the component stars suggest that both components have reached the final stage of their Main Sequence lifetime and proceed to the Giant Branch. KEYWORDS: Absolute parameters, Cru OB1, eclipsing binaries, MQ Cen, OB associations, spectral disentangling.

  2. Molecular Clouds and OB Association in the Sco-Cen-Lup Region

    NASA Astrophysics Data System (ADS)

    Tachihara, K.; Neuhäuser, R.; Toyoda, S.; Onishi, T.; Mizuno, A.; Fukui, Y.

    With the NANTEN radio telescope, we have surveyed more than 500 deg2 around the Scorpius-Centaurus-Lupus region in 12CO, and the molecular gas distribution has been revealed. Total mass of ~ 104 Msolar and ~ 100 of small clouds have been detected as the Lupus cloud complex. There are two subgroups of Sco OB2 association, Upper-Sco and Upper-Cen-Lup (e.g., de Geus 1989), and many star-forming scenarios controlled by the OB association are suggested (e.g., de Geus 1992). In the association, a large number of coexisting T Tauri stars (TTSs) have been found with the ROSAT All Sky Survey (Krautter et al. 1997; Wichmann et al. 1997; Preibisch et al. 1999). On the other hand, active cluster formation is taking place in ρ Oph cloud core and Lupus 3 (Tachihara et al. 1996; 2000). In order to understand the star-formation history and the effect of OB association on cloud formation and dissipation, we investigate the distribution of the TTSs, molecular clouds, and OB stars. The distribution of the OB stars and the molecular clouds show clear anti-correlation. On the other hand, considerable fractions of the TTSs distribute away from the molecular clouds (so-called ``isolated TTSs''). These suggest that molecular clouds have been dissipated quickly due to the OB stars. On the other hand, there is an expanding HI shell centered in Upper-Sco, and the Lupus cloud complex exists between the shell and the Upper-Cen-Lup. The young star clusters are located at the edge of the shell, which suggests triggered star formation. Molecular clouds and star-formation seem to be affected strongly by the present OB stars and the past supernova explosion.

  3. Towards a better way of quantifying uncertainty in the E-OBS temperature dataset

    NASA Astrophysics Data System (ADS)

    Cornes, Richard; van den Besselaar, Else; Jones, Phil; van der Schrier, Gerard; Verver, Ge

    2016-04-01

    E-OBS is a widely used gridded dataset that provides daily resolution data from 1950 to "present-day" across Europe and is constructed by interpolating station data contained in the ECA&D database. While E-OBS provides several gridded variables, the focus in this study is uncertainty in the gridded temperature data. A measure of error is currently included in the dataset that aims to provide an estimate of the uncertainty of the data at each grid-box for each day. This uncertainty estimate is mainly a function of the density of the input station data and is particularly important as the quantity of these data vary spatially and temporally; this has a large effect on the reliability of the gridded data. However, the uncertainty measure currently provided in E-OBS remains one of the least-used components of the dataset by end-users. This uncertainty calculation is based on a combination of monthly error values, using Bayesian standard error estimates derived from monthly climatology values, and daily error values derived from interpolation variance. In this analysis we present an alternative way of quantifying uncertainty in the E-OBS data for the three temperature variables (maximum, minimum and mean daily temperature) using a multiple realization (ensemble) approach. The established E-OBS gridding method is retained but an ensemble of grids is produced using conditional stochastic simulation. This ensemble approach would seem to be the best way of incorporating uncertainty measures into the existing E-OBS gridding scheme, whilst providing a measure of uncertainty that should be of use to many users of the E-OBS data.

  4. Parameterization of DFTB3/3OB for Sulfur and Phosphorus for Chemical and Biological Applications

    PubMed Central

    2015-01-01

    We report the parametrization of the approximate density functional tight binding method, DFTB3, for sulfur and phosphorus. The parametrization is done in a framework consistent with our previous 3OB set established for O, N, C, and H, thus the resulting parameters can be used to describe a broad set of organic and biologically relevant molecules. The 3d orbitals are included in the parametrization, and the electronic parameters are chosen to minimize errors in the atomization energies. The parameters are tested using a fairly diverse set of molecules of biological relevance, focusing on the geometries, reaction energies, proton affinities, and hydrogen bonding interactions of these molecules; vibrational frequencies are also examined, although less systematically. The results of DFTB3/3OB are compared to those from DFT (B3LYP and PBE), ab initio (MP2, G3B3), and several popular semiempirical methods (PM6 and PDDG), as well as predictions of DFTB3 with the older parametrization (the MIO set). In general, DFTB3/3OB is a major improvement over the previous parametrization (DFTB3/MIO), and for the majority cases tested here, it also outperforms PM6 and PDDG, especially for structural properties, vibrational frequencies, hydrogen bonding interactions, and proton affinities. For reaction energies, DFTB3/3OB exhibits major improvement over DFTB3/MIO, due mainly to significant reduction of errors in atomization energies; compared to PM6 and PDDG, DFTB3/3OB also generally performs better, although the magnitude of improvement is more modest. Compared to high-level calculations, DFTB3/3OB is most successful at predicting geometries; larger errors are found in the energies, although the results can be greatly improved by computing single point energies at a high level with DFTB3 geometries. There are several remaining issues with the DFTB3/3OB approach, most notably its difficulty in describing phosphate hydrolysis reactions involving a change in the coordination number of

  5. Quantification of the full length leptin receptor (OB-Rb) in human brown and white adipose tissue.

    PubMed

    Kutoh, E; Boss, O; Levasseur, F; Giacobino, J P

    1998-01-01

    Levels of expression of the leptin receptor (OB-R) splice variants have been studied in human omental white and perirenal brown adipose tissues by reverse transcription-PCR. The level of mRNA expression of the full length form (OB-Rb) was approximately 15% of that of the sum of all splice variants in white or brown adipose tissue. In an attempt to quantify the gene expression of OB-Rb in human white adipose tissue, a quantitative competitive PCR technique was developed, using oligonucleotide primers designed for OB-Rb and an internal standard for a "MIMIC" competition strategy. The levels of expression of OB-Rb mRNA in the omental fat of lean and obese patients were compared and no difference could be observed between the two groups. The quantitative RT-PCR technique allows for a fast and accurate measurement of the expression of the OB-Rb mRNA in small tissue samples.

  6. Efforts to Improve Immunization Coverage during Pregnancy among Ob-Gyns

    PubMed Central

    Jones, Katherine M.; Carroll, Sarah; Hawks, Debra; McElwain, Cora-Ann; Schulkin, Jay

    2016-01-01

    Background. Influenza and Tdap vaccines are vital factors for improving maternal and neonatal health outcomes. Methods. A prospective, longitudinal study was conducted to determine whether the American College of Obstetricians and Gynecologists' (ACOG's) efforts to increase ob-gyn use of their immunization toolkits and vaccination administration were successful. Pre- and postintervention questionnaires were mailed to a random sample of 1,500 ACOG members between August 2012 and July 2015. Results. Significantly more postintervention survey ob-gyns reported that they received the immunization toolkits than preintervention survey ob-gyns (84.5% versus 67.0%, p < .001). The large majority of ob-gyns from both surveys (76.9% versus 78.9%) reported that they offered or planned to offer influenza vaccinations to their patients for the 2012-2013 and 2014-2015 flu seasons. Postintervention survey respondents were significantly more likely than preintervention survey participants to report that they routinely offer Tdap vaccinations to all patients during pregnancy (76.8% versus 59.3%, p < .001). Conclusion. ACOG's efforts to improve ob-gyn use of immunization toolkits and vaccine administration appear to have been successful in several ways. ACOG's toolkits are an example of an effective intervention to overcome barriers to offering vaccines and help improve influenza and Tdap immunization coverage for pregnant women. PMID:26924918

  7. Leptin promotes proliferation and metastasis of human gallbladder cancer through OB-Rb leptin receptor.

    PubMed

    Zou, Hao; Liu, Yunxia; Wei, Dong; Wang, Tao; Wang, Kun; Huang, Songquan; Liu, Lixin; Li, Yuehua; Ge, Jiayun; Li, Xiao; Zhu, Hong; Wang, Lianmin; Zhao, Songling; Zhang, Xiaowen; Wang, Lin

    2016-07-01

    Emerging evidence has shown that leptin, an adipocyte-derived cytokine that is closely associated with obesity, play a significant role in carcinogenesis and tumorigenesis. However, its impact on gallbladder cancer (GBC) remains unclear. In this study, we firstly found that leptin and its functional receptor OB-Rb were significantly co-expressed in human GBC tissues and cell lines, the content of which were higher than those in normal human gallbladder tissues. Treatment with leptin promoted the proliferation, migration and invasion of GBC cells, which were attenuated by OB-Rb shRNA. Blocking in the G2/M period of cell cycle, increasing of MMP3 and MMP9, increasing of VEGF-C/D, activation of SOCS3/JAK2/p-STAT3 pathway was demonstrated after treatment with leptin. All of these positive responses were attenuated by OB-Rb receptor shRNA. Taken together, our findings suggest that leptin promoted the proliferation, migration and invasion of GBC cells by increasing OB-Rb expression through the SOCS3/JAK2/p-STAT3 signal pathway. Targeting the leptin/OB-Rb axis could be an attractive therapeutic strategy for treatment of GBC.

  8. Spectroscopy of Intermediate Mass Members of the Orion OB1a and b associations

    NASA Astrophysics Data System (ADS)

    Walter, Frederick M.; Sherry, William; Kim, Serena; Brittain, Sean

    2010-08-01

    OB associations are ideal sites for probing the formation of low mass stars. There has been little systematic study of the low mass stars in the largest nearby OB association, Orion OB1, aside from the Orion Nebula and σ Ori clusters. In an attempt to map the mass function and its spatial variation from 40 to below 0.01 M_⊙, we have completed a near-IR (JHK) survey of about 18 square degrees in the Orion OB1a and OB1b associations using the CPAPIR camera on the SMARTS/CTIO 1.5m telescope, supplemented by a VRI survey using the Steward Observatory Bok/90Prime and the KPNO 4m/MOSAIC cameras, as well as deep 4m/NEWFIRM images. The photometry reveals thousands of candidate association members. We request time with HYDRA to obtain spectra of the brighter candidate members (V<19), in order to confirm youth and membership from Li abundances, chromospheric activity, and radial velocities.

  9. Experimental demonstration of OSPF-TE extensions in muiti-domain OBS networks connected by GMPLS network

    NASA Astrophysics Data System (ADS)

    Tian, Chunlei; Yin, Yawei; Wu, Jian; Lin, Jintong

    2008-11-01

    The interworking network of Generalized Multi-Protocol Label Switching (GMPLS) and Optical Burst Switching (OBS) is attractive network architecture for the future IP/DWDM network nowadays. In this paper, OSPF-TE extensions for multi-domain Optical Burst Switching networks connected by GMPLS controlled WDM network are proposed, the corresponding experimental results such as the advertising latency are also presented by using an OBS network testbed. The experimental results show that it works effectively on the OBS/GMPLS networks.

  10. Modeling the potential radionuclide transport by the Ob and Yenisey Rivers to the Kara Sea.

    PubMed

    Paluszkiewicz, T; Hibler, L F; Richmond, M C; Bradley, D J; Thomas, S A

    2001-01-01

    A major portion of the former Soviet Union (FSU) nuclear program is located in the West Siberian Basin. Among the many nuclear facilities are three production reactors and the spent nuclear fuel reprocessing sites, Mayak, Tomsk-7, and Krasnoyarsk-26, which together are probably responsible for the majority of the radioactive contamination found in the Ob and Yenisey River systems that feed into the Arctic Ocean through the Kara Sea. This manuscript describes ongoing research to estimate radionuclide fluxes to the Kara Sea from these river systems. Our approach is to apply a hierarchy of simple models that use existing and forthcoming data to quantify the transport and fate of radionuclide contaminants via various environmental pathways. We present an initial quantification of the contaminant inventory, hydrology, meteorology, and sedimentology of the Ob River system and preliminary conclusions from portions of the Ob River model.

  11. An X-ray and Infrared Hunt for New Candidate Galactic OB Stars

    NASA Astrophysics Data System (ADS)

    Povich, Matthew S.; Alexander, Michael J.; Busk, Heather; Hanes, Richard J.; Feigelson, Eric; McSwain, M. Virginia; Townsley, Leisa K.

    2016-01-01

    Most young, massive OB stars produce X-ray emission through a variety of wind-driven shock processes, and individual massive stars are detectable out to several kpc distances in the Galactic plane using high-resolution imaging observations from the Chandra X-ray Observatory. We have developed a technique to identify known and new candidate OB stars by fitting model stellar atmospheres to the broadband infrared spectral energy distributions of X-ray-identified stars. Using this technique, we identified 94 candidate O- and early B-type stars in the Carina Nebula and an additional 98 candidates in 11 other Galactic Massive Star-Forming Regions. Visible-light and near-infrared follow-up spectroscopy of these candidates is ongoing, and initial results indicate that a majority of candidate massive stars will be spectroscopically confirmed as OB stars.

  12. Evaluation of metal content in perch of the Ob River basin

    NASA Astrophysics Data System (ADS)

    Osipova, N. A.; Stepanova, K. D.; Matveenko, I. A.

    2015-11-01

    The geochemical features of river perch in the River Ob basin have been studied (the upper and middle reaches of the Ob River and the lower reach of the Tom River). The contents of Ag, Bi, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Sn, W, Zn, Hg in perch's soft tissue are defined by the methods of ICP AES and stripping voltammetry, that of mercury in bones - by the atomic absorption method using mercury analyzer PA-915+. The distribution series of metal absolute concentrations in perch's soft tissue from the Ob River basin are plotted: Fe > Zn > Cu > Mn, typical for uncontaminated or slightly metal contaminated water bodies. In soft tissue of the studied samples the metal content does not exceed the permissible values. The mercury content in bones of studied samples is in the range 0,036-0,556 mg/kg. The mercury concentration is higher in bones in comparison with soft tissue in all samples.

  13. Gamma ray bursts versus OB associations: do they trigger star formation?

    NASA Astrophysics Data System (ADS)

    Efremov, Yu. N.; Ehlerová, S.; Palouš , J.

    1999-10-01

    We discuss differences in shapes, expansion velocities and fragmentation times of structures created by an energy deposition from a single Gamma-Ray Burst (GRB) and an OB association to the ISM. After the initial inflation, supershells produced by GRBs are almost static or slowly expanding, contrary to more rapidly expanding supershells created by OB associations. We discuss the position of the energy source relative to the symmetry plane of the galaxy: observed arc-like structures can be the most dense part of structures formed by an expansion from a source above or below the galactic plane. Arcs may also form if the expansion takes place inside a giant HI cloud. We try to reproduce the size, the age, and the average distance between OB associations in the Sextant region at the edge of LMC 4.

  14. Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b

    SciTech Connect

    Stein, Lisa Y.; Yoon, Sukhwan; Semrau, Jeremy D.; DiSpiritto, Alan A.; Crombie, Andrew; Murrell, J.; Vuilleumier, Stephane; Kalyuzhnaya, Marina G.; Den Camp, Huub J. M. Op; Bringel, Francoise O.; Bruce, David; Cheng, Jan-Fang; Copeland, A; Goodwin, Lynne A.; Han, Cliff; Hauser, Loren John; Jetten, MSM; Lajus, Aurelie; Lapidus, Alla L.; Lucas, Susan; Medigue, Claudine; Woyke, Tanja; Zeytun, Ahmet; Klotz, Martin G

    2010-01-01

    Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.

  15. Very Low Mass Objects in Orion OB1a and b

    NASA Astrophysics Data System (ADS)

    Walter, Frederick M.; Faherty, Jacqueline K.; Sherry, William H.; Brittain, Sean

    2009-02-01

    We present initial results of a search for the substellar mass population of the Orion OB1 a and b associations, based on a deep optical/near-IR survey. We are complete to about 1 magnitude fainter than the 2MASS survey at K. Global comparison of the K vs. J-K color-magnitude diagram shows an excess of objects with K>14.4 (the substellar limit in Orion OB1b) and 0.8OB1b. Near-IR spectra confirm spectral types near M7, and show the triangular H-band continuum shape associated with young (low gravity) objects. Space densities of these objects exceed a few hundred/deg2 near δ Orionis.

  16. Gas flow and generation of x ray emission in WR+OB binaries

    NASA Technical Reports Server (NTRS)

    Usov, V. V.

    1991-01-01

    The supersonic flow of the ionized gas in WR+OB binaries and X-ray generation are considered. X-ray emission is caused by gas heating up to temperatures of 10(exp 7) to 10(exp 8) K behind the front of shock waves. These are found in the collision of gas flowing out from the WR star with either the OB star's surface or the gas of the OB star's wind. The distribution of temperature and concentration behind the shock front are obtained. Using these distributions, the spectral power of bremsstrahlung X-ray emission of hot gas is calculated. Possible reasons that lead to a considerable difference between the observed parameters of X-ray emission of the WR binary of V 444 Cygni and the theoretically expected are discussed.

  17. The performance study of oxide by-passed(OB) lateral double diffused MOSFET

    NASA Astrophysics Data System (ADS)

    Tang, Pan-pan

    2016-10-01

    An SOI LDMOS device structure with Oxide By-passed(OB) was investigated and its breakdown mechanism and characteristic of structure was analyzed. Its performance was verified by 3D numerical simulation with SILVACO TCAD software. The simulated results show that the electrical field element of the device is modulated by the concept of similar Superjunction(SJ) structure. Compared with the SJ LDMOS device, OB LDMOS obtains the same breakdown voltage, simultaneously the specific on-resistance of the OB LDMOS reduces from 3.81mΩ·cm2 to 1.96mΩ·cm2, except for achieving comparable performance and overcoming the high aspect ratio of fabrication structure and the difficulty of accurate concentration match of SJ LDMOS.

  18. Long-Acting PASylated Leptin Ameliorates Obesity by Promoting Satiety and Preventing Hypometabolism in Leptin-Deficient Lep(ob/ob) Mice.

    PubMed

    Bolze, Florian; Morath, Volker; Bast, Andrea; Rink, Nadine; Schlapschy, Martin; Mocek, Sabine; Skerra, Arne; Klingenspor, Martin

    2016-01-01

    Body weight loss of Lep(ob/ob) mice in response to leptin is larger than expected from the reduction in energy intake alone, suggesting a thermogenic action of unknown magnitude. We exploited the superior pharmacological properties of a novel long-acting leptin prepared via PASylation to study the contribution of its anorexigenic and thermogenic effects. PASylation, the genetic fusion of leptin with a conformationally disordered polypeptide comprising 600 Pro/Ala/Ser (PAS) residues, provides a superior way to increase the hydrodynamic volume of the fusion protein, thus retarding kidney filtration and extending plasma half-life. Here a single PAS(600)-leptin injection (300 pmol/g) resulted in a maximal weight reduction of 21% 6 days after application. The negative energy balance of 300 kJ/(4 d) was driven by a decrease in energy intake, whereas energy expenditure remained stable. Mice that were food restricted to the same extent showed an energy deficit of only 220 kJ/(4 d) owing to recurring torpor bouts. Therefore, the anorexigenic effect of PAS(600)-leptin contributes 75% to weight loss, whereas the thermogenic action accounts for 25% by preventing hypometabolism. In a second experiment, just four injections of PAS(600)-leptin (100 pmol/g) administered in 5- to 6-day intervals rectified the Lep(ob/ob) phenotype. In total, 16 nmol of PAS(600)-leptin per mouse triggered a weight loss of 43% within 20 days and normalized hypothermia and glucose homeostasis as well as hepatic steatosis. The beneficial properties of PAS(600)-leptin are substantiated by a comparison with previous studies in which approximately 400 nmol (∼25-fold) unmodified leptin was mandatory to achieve similar improvements.

  19. Interferometric imaging of crustal structure from wide-angle multicomponent OBS-airgun data

    NASA Astrophysics Data System (ADS)

    Shiraishi, K.; Fujie, G.; Sato, T.; Abe, S.; Asakawa, E.; Kodaira, S.

    2015-12-01

    In wide-angle seismic surveys with ocean bottom seismograph (OBS) and airgun, surface-related multiple reflections and upgoing P-to-S conversions are frequently observed. We applied two interferometric imaging methods to the multicomponent OBS data in order to highly utilize seismic signals for subsurface imaging.First, seismic interferometry (SI) is applied to vertical component in order to obtain reflection profile with multiple reflections. By correlating seismic traces on common receiver records, pseudo seismic data are generated with virtual sources and receivers located on all original shot positions. We adopt the deconvolution SI because source and receiver spectra can be canceled by spectral division. Consequently, gapless reflection images from just below the seafloor to the deeper are obtained.Second, receiver function (RF) imaging is applied to multicomponent OBS data in order to image P-to-S conversion boundary. Though RF is commonly applied to teleseismic data, our purpose is to extract upgoing PS converted waves from wide-angle OBS data. The RF traces are synthesized by deconvolution of radial and vertical components at same OBS location for each shot. Final section obtained by stacking RF traces shows the PS conversion boundaries beneath OBSs. Then, Vp/Vs ratio can be estimated by comparing one-way traveltime delay with two-way traveltime of P wave reflections.We applied these methods to field data sets; (a) 175 km survey in Nankai trough subduction zone using 71 OBSs with from 1 km to 10 km intervals and 878 shots with 200 m interval, and (b) 237 km survey in northwest pacific ocean with almost flat layers before subduction using 25 OBSs with 6km interval and 1188 shots with 200 m interval. In our study, SI imaging with multiple reflections is highly applicable to OBS data even in a complex geological setting, and PS conversion boundary is well imaged by RF imaging and Vp/Vs ratio distribution in sediment is estimated in case of simple structure.

  20. VizieR Online Data Catalog: OB association members in ACT+TRC Catalogs (Hoogerwerf, 2000)

    NASA Astrophysics Data System (ADS)

    Hoogerwerf, R.

    2000-05-01

    The Hipparcos Catalogue (Cat. I/239) contains members of nearby OB associations brighter than 12th magnitude in V. However, membership lists are complete only to magnitude V=7.3. In this paper we discuss whether proper motions listed in the `Astrographic Catalogue+Tycho' reference catalogue (ACT, Cat. I/246) and the Tycho Reference Catalogue (TRC, Cat. I/250), which are complete to V~10.5mag, can be used to find additional association members. Proper motions in the ACT/TRC have an average accuracy of ~3mas/yr. We search for ACT/TRC stars which have proper motions consistent with the spatial velocity of the Hipparcos members of the nearby OB associations already identified by de Zeeuw et al. (1999, Cat. J/AJ/117/354). These stars are first selected using a convergent-point method, and then subjected to further constraints on the proper-motion distribution, magnitude and colour to narrow down the final number of candidate members. Monte Carlo simulations show that the proper-motion distribution, magnitude, and colour constraints remove ~97% of the field stars, while at the same time retain more than 90% of the cluster stars. The procedure has been applied to five nearby associations: the three subgroups of Sco OB2, plus Per OB3 and Cep OB6. In all cases except Cep OB6, we find evidence for new association members fainter than the completeness limit of the Hipparcos Catalogue. However, narrow-band photometry and/or radial velocities are needed to pinpoint the cluster members, and to study their physical characteristics. (1 data file).

  1. ObRb downregulation increases breast cancer cell sensitivity to tamoxifen.

    PubMed

    Qian, Yingying; Shi, Dongmin; Qiu, Jinrong; Zhu, Fang; Qian, Jing; He, Shaohua; Shu, Yongqian; Yin, Yongmei; Chen, Xiaofeng

    2015-09-01

    Leptin is a potent adipokine that plays an important role in the progression of breast cancer and interferes with the action of tamoxifen. We investigated the molecular mechanism underlying the effect of leptin on tamoxifen resistance in breast cancer cells that express leptin receptor (ObRb), and evaluated the impact of ObRb suppression on tamoxifen treatment in MCF-7 and tamoxifen-resistant (TAM-R) cells. Leptin-induced signaling pathway activation was examined by qRT-PCR and Western blotting. Chromatin immunoprecipitation assays were performed to further examine the binding of estrogen receptor (ER) α on the promoter of cyclin D1 (CCND1) gene. The effects of combined ObRb knockdown and tamoxifen treatment were evaluated in MCF-7 and TAM-R cells. We found that the enhanced proliferation effects induced by leptin were related to extracellular-signal-regulated kinase (ERK) 1/2 and signal transducers and activators of transcription (STAT) 3 signaling pathway activation and CCND1 upregulation. Leptin enhanced CCND1 gene transcription by inducing the binding of ERα to the promoter of CCND1 gene. ObRb knockdown significantly enhanced the inhibitory effects of tamoxifen on TAM-R cell proliferation and survival. This study suggested that long-term endocrine therapy facilitates leptin and ObRb overexpression in breast cancer cells, which attenuates the inhibitory effect of tamoxifen by activating both the ERK1/2 and STAT3 signaling pathways and upregulating CCND1 gene expression. Combination therapy involving ObRb knockdown and tamoxifen treatment may be an alternative therapeutic option for tamoxifen-resistant breast cancer.

  2. Reconstruction of Ob River, Russia, discharge from ring widths of floodplain trees

    NASA Astrophysics Data System (ADS)

    Agafonov, Leonid I.; Meko, David M.; Panyushkina, Irina P.

    2016-12-01

    The Ob is the third largest Eurasian river supplying heat and freshwater to the Arctic Ocean. These inputs influence water salinity, ice coverage, ocean temperatures and ocean circulation, and ultimately the global climate system. Variability of Ob River flow on long time scales is poorly understood, however, because gaged flow records are short. Eleven tree-ring width chronologies of Pinus sibirica and Larix sibirica are developed from the floodplain of the Lower Ob River, analyzed for hydroclimatic signal and applied as predictors in a regression model to reconstruct 8-month average (December-July) discharge of the Ob River at Salekhard over the interval 1705-2012 (308 yrs). Correlation analysis suggests the signal for discharge comes through air temperature: high discharge and floodplain water levels favor cool growing-season air temperature, which limits tree growth for the sampled species at these high latitudes. The reconstruction model (R2 = 0.31, 1937-2009 calibration period) is strongly supported by cross-validation and analysis of residuals. Correlation of observed with reconstructed discharge improves with smoothing. The long-term reconstruction correlates significantly with a previous Ob River reconstruction from ring widths of trees outside the Ob River floodplain and extends that record by another century. Results suggest that large multi-decadal swings in discharge have occurred at irregular intervals, that variations in the 20th and 21st centuries have been within the envelope of natural variability of the past 3 centuries, and that discharge data for 1937-2009 underestimate both the variability and persistence of discharge in the last 3 centuries. The reconstruction gives ecologists, climatologists and water resource planners a long-term context for assessment of climate change impacts.

  3. Near Infrared Diffuse Interstellar Bands Toward the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    Hamano, Satoshi; Kobayashi, Naoto; Kondo, Sohei; Sameshima, Hiroaki; Nakanishi, Kenshi; Ikeda, Yuji; Yasui, Chikako; Mizumoto, Misaki; Matsunaga, Noriyuki; Fukue, Kei; Yamamoto, Ryo; Izumi, Natsuko; Mito, Hiroyuki; Nakaoka, Tetsuya; Kawanishi, Takafumi; Kitano, Ayaka; Otsubo, Shogo; Kinoshita, Masaomi; Kawakita, Hideyo

    2016-04-01

    We obtained the near-infrared (NIR) high-resolution (R ≡ λ/Δλ ∼ 20,000) spectra of the seven brightest early-type stars in the Cygnus OB2 association for investigating the environmental dependence of diffuse interstellar bands (DIBs). The WINERED spectrograph mounted on the Araki 1.3 m telescope in Japan was used to collect data. All 20 of the known DIBs within the wavelength coverage of WINERED (0.91 < λ < 1.36 μm) were clearly detected along all lines of sight because of their high flux density in the NIR wavelength range and the large extinction. The equivalent widths (EWs) of DIBs were not correlated with the column densities of C2 molecules, which trace the patchy dense component, suggesting that the NIR DIB carriers are distributed mainly in the diffuse component. On the basis of the correlations among the NIR DIBs both for stars in Cyg OB2 and stars observed previously, λλ10780, 10792, 11797, 12623, and 13175 are found to constitute a “family,” in which the DIBs are correlated well over the wide EW range. In contrast, the EW of λ10504 is found to remain almost constant over the stars in Cyg OB2. The extinction estimated from the average EW of λ10504 (AV ∼ 3.6 mag) roughly corresponds to the lower limit of the extinction distribution of OB stars in Cyg OB2. This suggests that λ10504 is absorbed only by the foreground clouds, implying that the carrier of λ10504 is completely destroyed in Cyg OB2, probably by the strong UV radiation field. The different behaviors of the DIBs may be caused by different properties of the DIB carriers.

  4. Views of American OB/GYNs on the ethics of prenatal whole-genome sequencing.

    PubMed

    Bayefsky, Michelle J; White, Amina; Wakim, Paul; Hull, Sara Chandros; Wasserman, David; Chen, Stephanie; Berkman, Benjamin E

    2016-12-01

    Given public demand for genetic information, the potential to perform prenatal whole-genome sequencing (PWGS) non-invasively in the future, and decreasing costs of whole-genome sequencing, it is likely that OB/GYN practice will include PWGS. The goal of this project was to explore OB/GYNs' views on the ethical issues surrounding PWGS and their preparedness for counseling patients on its use. A national survey was administered to 2500 members of American Congress of Obstetricians and Gynecologists. A total of 1114 respondents completed the survey (response rate = 45%). OB/GYNs are most concerned with ordering non-medical fetal genetic information, are worried about increasing parental anxiety, and feel it is appropriate to be directive when counseling parents about PWGS. Furthermore, most OB/GYNs have limited knowledge of genetics, rely heavily on genetic counselors and would like more guidance regarding the clinical adoption of PWGS. OB/GYNs do not completely accept or reject PWGS, but a substantial number have significant ethical and practical concerns. They are most concerned with issues that will directly affect their practices and interactions with patients, such as increasing parental anxiety and costs of care. Professional guidance would be instrumental in directing the adoption of PWGS and alleviating the ethical burden posed by PWGS on individual OB/GYNs. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

  5. NEAR INFRARED DIFFUSE INTERSTELLAR BANDS TOWARD THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Hamano, Satoshi; Kondo, Sohei; Sameshima, Hiroaki; Nakanishi, Kenshi; Kawakita, Hideyo; Kobayashi, Naoto; Ikeda, Yuji; Yasui, Chikako; Mizumoto, Misaki; Matsunaga, Noriyuki; Fukue, Kei; Yamamoto, Ryo; Izumi, Natsuko; Mito, Hiroyuki; Nakaoka, Tetsuya; Kawanishi, Takafumi; Kitano, Ayaka; Otsubo, Shogo; Kinoshita, Masaomi

    2016-04-10

    We obtained the near-infrared (NIR) high-resolution (R ≡ λ/Δλ ∼ 20,000) spectra of the seven brightest early-type stars in the Cygnus OB2 association for investigating the environmental dependence of diffuse interstellar bands (DIBs). The WINERED spectrograph mounted on the Araki 1.3 m telescope in Japan was used to collect data. All 20 of the known DIBs within the wavelength coverage of WINERED (0.91 < λ < 1.36 μm) were clearly detected along all lines of sight because of their high flux density in the NIR wavelength range and the large extinction. The equivalent widths (EWs) of DIBs were not correlated with the column densities of C{sub 2} molecules, which trace the patchy dense component, suggesting that the NIR DIB carriers are distributed mainly in the diffuse component. On the basis of the correlations among the NIR DIBs both for stars in Cyg OB2 and stars observed previously, λλ10780, 10792, 11797, 12623, and 13175 are found to constitute a “family,” in which the DIBs are correlated well over the wide EW range. In contrast, the EW of λ10504 is found to remain almost constant over the stars in Cyg OB2. The extinction estimated from the average EW of λ10504 (A{sub V} ∼ 3.6 mag) roughly corresponds to the lower limit of the extinction distribution of OB stars in Cyg OB2. This suggests that λ10504 is absorbed only by the foreground clouds, implying that the carrier of λ10504 is completely destroyed in Cyg OB2, probably by the strong UV radiation field. The different behaviors of the DIBs may be caused by different properties of the DIB carriers.

  6. TCP traffic carrying capabilities of OBS-based hypercubes for datacenters

    NASA Astrophysics Data System (ADS)

    Argibay-Losada, Pablo Jesus; Qiao, Chunming; Peng, Limei; Tang, Wan

    2011-11-01

    High power consumption due to O/E/O processing in many conventional electronic datacenter networks can be greatly decreased by a suitable use of all-optical switching technologies. OBS is specially suited to perform this task given its bursty-data traffic friendly mode of operation. In this paper, we evaluate through analysis and simulation the performance of both OBS and electronic networks when used to carry TCP flows inside a 6D-hypercube, a highly symmetrical topology representative of datacenter networks.

  7. Analyzing the Effects of Nitrogen Deficiency on the PHB Production of Methylosinus trichosporium OB3b

    NASA Astrophysics Data System (ADS)

    Kyauk, E.

    2011-12-01

    Polyhydroxybutyrate (PHB) is a biodegradable thermoplastic that is produced by various microorganisms. Because of its potential to replace conventional plastics, it has been closely researched in the past few years. Methanotrophic bacteria, bacteria that consume methane, produce this bioplastic when it lacks certain nutrients. The utilization of methane to produce PHB shows much promise as methane is a cheap, plentiful gas. In this study, we observed the methanotroph, Methylosinus trichosporium OB3b , and its yield of PHB in the absence of nitrogen. The optical density of Methylosinus trichosporium OB3b was measured in order to observe cell growth and PHB production patterns over a 48 hour period.

  8. Differentiated service in OBS networks using a dynamic FDL bank partitioning algorithm.

    PubMed

    Yonggyu, Lee; Kim, Namuk; Kim, Jaegwan; Ahn, Junseop; Kang, Minho

    2007-05-14

    In order to solve the differentiated service problem in optical burst switching (OBS) networks, we propose a dynamic fiber delay line (FDL) bank partitioning algorithm, which divides a FDL bank into several groups, using a feed-forward output buffering architecture. In the analysis, three classes and groups are considered for traffic and FDL, respectively, and each group is assigned to each class. This paper shows that the loss differentiation in OBS networks is easily accomplished in Poisson traffic environments when our dynamic algorithm is adopted.

  9. OBS development for long term observation in the Marmara Sea, NW Turkey

    NASA Astrophysics Data System (ADS)

    Takahashi, Narumi; Shimizu, Satoshi; Maekawa, Takuya; Kalafat, Dogan; Pinar, Ali; Citak, Seckin; Kaneda, Yoshiyuki

    2015-04-01

    We have carried out a collaboration study between Japan and Turkey since 2013, which is one of SATREPS projects, "Earthquake and Tsunami Disaster Mitigation in The Marmara Region and Disaster Education in Turkey". The main objective of this project is to reduce risk brought by earthquakes and tsunamis. In particular, the North Anatolian Fault system runs through the Marmara sea and it is expected that the seismic gap exists there according to past seismic studies. The details of seismicity distribution in the Marmara Sea is, however, still insufficient to construct fault model along the active faults. Therefore, we prepare ten ocean bottom seismographs (OBSs) to realize long term observation. We aim to identify size and depth of seismogenic zones using micro seismicity. In addition, we need to cover relative broad area from off-shore Istanbul city to the western end of the Marmara Sea. To clear these conditions, OBS specifications we need are high dynamic range and low instrument noise to observe micro seismicity, low electrical consumption to realize long term observation of over one year, high cost performance to cover the broad area for OBS installation, low cost implementation, and good operability to treat by relatively small number of persons. All items, which are three components velocity sensor, batteries, a recorder, a GPS receiver, a transponder and its transducer to control OBS retrieval, a flasher and a beacon, are installed in the 17 inches glass sphere. The natural frequency of the velocity sensor is 4.5 Hz and the frequency range of our OBS is from 4.5 Hz to 250 Hz. Data sampling is selectable among 100 Hz, 250 Hz and 500 Hz. Because our OBS is deployed by free fall, accuracy of the OBS clock is essentially one of important factors, and it is less than 0.1 ppm. And the resolution of A/D conversion performed on the recorder is 24 bit and we keep the dynamic range of over 135 dB. These data is stored on a semiconductor memory and the capacity is over

  10. Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds.

    PubMed

    Park, Young-Jun; Hol, Wim G J

    2012-11-01

    Trypanosomatids form a group of protozoa which contain parasites of human, animals and plants. Several of these species cause major human diseases, including Trypanosoma brucei which is the causative agent of human African trypanosomiasis, also called sleeping sickness. These organisms have many highly unusual features including a unique U-insertion/deletion RNA editing process in the single mitochondrion. A key multi-protein complex, called the ∼20S editosome, or editosome, carries out a cascade of essential RNA-modifying reactions and contains a core of 12 different proteins of which six are the interaction proteins A1 to A6. Each of these interaction proteins comprises a C-terminal OB-fold and the smallest interaction protein A6 has been shown to interact with four other editosome OB-folds. Here we report the results of a "linked OB-fold" approach to obtain a view of how multiple OB-folds might interact in the core of the editosome. Constructs with variants of linked domains in 25 expression and co-expression experiments resulted in 13 soluble multi-OB-fold complexes. In several instances, these complexes were more homogeneous in size than those obtained from corresponding unlinked OB-folds. The crystal structure of A3(OB) linked to A6 could be elucidated and confirmed the tight interaction between these two OB domains as seen also in our recent complex of A3(OB) and A6 with nanobodies. In the current crystal structure of A3(OB) linked to A6, hydrophobic side chains reside in well-defined pockets of neighboring OB-fold domains. When analyzing the available crystal structures of editosome OB-folds, it appears that in five instances "Pocket 1" of A1(OB), A3(OB) and A6 is occupied by a hydrophobic side chain from a neighboring protein. In these three different OB-folds, Pocket 1 is formed by two conserved sequence motifs and an invariant arginine. These pockets might play a key role in the assembly or mechanism of the editosome by interacting with hydrophobic

  11. Chronic treatment with exendin(9-39)amide indicates a minor role for endogenous glucagon-like peptide-1 in metabolic abnormalities of obesity-related diabetes in ob/ob mice.

    PubMed

    Green, B D; Irwin, N; Gault, V A; Bailey, C J; O'Harte, F P M; Flatt, P R

    2005-05-01

    Glucagon-like peptide-1 (GLP-1) is a potent insulinotropic hormone proposed to play a role in both the pathophysiology and treatment of type 2 diabetes. This study has employed the GLP-1 receptor antagonist, exendin-4(9-39)amide (Ex(9-39)) to evaluate the role of endogenous GLP-1 in genetic obesity-related diabetes and related metabolic abnormalities using ob/ob and normal mice. Acute in vivo antagonistic potency of Ex(9-39) was confirmed in ob/ob mice by blockade of the insulin-releasing and anti-hyperglycaemic actions of intraperitoneal GLP-1. In longer term studies, ob/ob mice were given once daily injections of Ex(9-39) or vehicle for 11 days. Feeding activity, body weight, and both basal and glucose-stimulated insulin secretion were not significantly affected by chronic Ex(9-39) treatment. However, significantly elevated basal glucose concentrations and impaired glucose tolerance were evident at 11 days. These disturbances in glucose homeostasis were independent of changes of insulin sensitivity and reversed by discontinuation of the Ex(9-39) for 9 days. Similar treatment of normal mice did not affect any of the parameters measured. These findings illustrate the physiological extrapancreatic glucose-lowering actions of GLP-1 in ob/ob mice and suggest that the endogenous hormone plays a minor role in the metabolic abnormalities associated with obesity-related diabetes.

  12. Advective transport observations with MODPATH-OBS--documentation of the MODPATH observation process

    USGS Publications Warehouse

    Hanson, R.T.; Kauffman, L.K.; Hill, M.C.; Dickinson, J.E.; Mehl, S.W.

    2013-01-01

    The MODPATH-OBS computer program described in this report is designed to calculate simulated equivalents for observations related to advective groundwater transport that can be represented in a quantitative way by using simulated particle-tracking data. The simulated equivalents supported by MODPATH-OBS are (1) distance from a source location at a defined time, or proximity to an observed location; (2) time of travel from an initial location to defined locations, areas, or volumes of the simulated system; (3) concentrations used to simulate groundwater age; and (4) percentages of water derived from contributing source areas. Although particle tracking only simulates the advective component of conservative transport, effects of non-conservative processes such as retardation can be approximated through manipulation of the effective-porosity value used to calculate velocity based on the properties of selected conservative tracers. This program can also account for simple decay or production, but it cannot account for diffusion. Dispersion can be represented through direct simulation of subsurface heterogeneity and the use of many particles. MODPATH-OBS acts as a postprocessor to MODPATH, so that the sequence of model runs generally required is MODFLOW, MODPATH, and MODPATH-OBS. The version of MODFLOW and MODPATH that support the version of MODPATH-OBS presented in this report are MODFLOW-2005 or MODFLOW-LGR, and MODPATH-LGR. MODFLOW-LGR is derived from MODFLOW-2005, MODPATH 5, and MODPATH 6 and supports local grid refinement. MODPATH-LGR is derived from MODPATH 5. It supports the forward and backward tracking of particles through locally refined grids and provides the output needed for MODPATH_OBS. For a single grid and no observations, MODPATH-LGR results are equivalent to MODPATH 5. MODPATH-LGR and MODPATH-OBS simulations can use nearly all of the capabilities of MODFLOW-2005 and MODFLOW-LGR; for example, simulations may be steady-state, transient, or a combination

  13. BOREAS TF-9 SSA-OBS Tower Flux, Meteorological, and Soil Temperature Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Massheder, Jonathan M.; Moncrieff, John B.; Rayment, Mark B.; Jarvis, Paul G.

    2000-01-01

    The BOREAS TF-9 team collected energy, carbon dioxide, and water vapor flux data at the BOREAS SSA-OBS site during the growing season of 1994 and most of the year for 1996. From the winter of 1995 to 1996, soil temperature data were also collected and provided. The data are available in tabular ASCII files.

  14. BOREAS TF-7 SSA-OBS Tower Flux and Meteorological Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Pattey, Elizabeth; Desjardins, Raymond L.

    2000-01-01

    The BOREAS TF-7 team collected meteorological data as well as energy, carbon dioxide, water vapor, methane, and nitrous oxide flux data at the BOREAS SSA-OBS site. The data were collected from 24-May to 19-Sep-1994. The data are available in tabular ASCII files.

  15. BOREAS TF-3 NSA-OBS Tower Flux, Meteorological, and Soil Temperature Data

    NASA Technical Reports Server (NTRS)

    Wofsy, Steven; Sutton, Doug; Goulden, Mike; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor)

    2000-01-01

    The BOReal Ecosystem-Atmosphere Study Tower Flux (BOREAS TF-3) team collected tower flux, surface meteorological, and soil temperature data at the BOREAS Northern Study Area-Old Black Spruce (NSA-OBS) site continuously from the March 1994 through October 1996. The data are available in tabular ASCII files.

  16. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b

    DOE PAGES

    Gu, Wenyu; Farhan Ul Haque, Muhammad; DiSpirito, Alan A.; ...

    2016-05-12

    It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium andmore » samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.« less

  17. AmeriFlux CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce

    DOE Data Explorer

    Black, T. Andrew [The University of British Columbia

    2017-01-01

    This is the AmeriFlux version of the carbon flux data for the site CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce. Site Description - 53.98717° N, 105.11779° W, elavation of 628.94 m, BOREAS 1994, 1996, BERMS climate measurements began Dec. 1996 and flux measurements in Apr. 1999

  18. Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47T

    SciTech Connect

    Elkins, James G; Lochner, Adriane; Hamilton-Brehm, Scott; Walston Davenport, Karen; Podar, Mircea; Brown, Steven D; Land, Miriam L; Hauser, Loren John; Klingeman, Dawn Marie; Raman, Babu; Goodwin, Lynne A.; Tapia, Roxanne; Meincke, Linda; Detter, J C; Bruce, David; Han, Cliff; Palumbo, Anthony Vito; Cottingham, Robert W; Keller, Martin; Graham, David E

    2010-01-01

    Caldicellulosiruptor obsidiansis OB47T (ATCC BAA-2073; JCM 16842) is an extremely thermophilic, anaerobic bacterium capable of hydrolyzing plant-derived polymers through the expression of multidomain/multifunctional hydrolases. The complete genome sequence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignocellulosic biomass hydrolysis at high temperatures.

  19. X-Ray Emission from Massive Stars in Cyg OB2

    NASA Astrophysics Data System (ADS)

    Rauw, G.; Nazé, Y.; Wright, N. J.; Drake, J. J.; Guarcello, M. G.; Prinja, R. K.; Peck, L. W.; Albacete Colombo, J. F.; Herrero, A.; Kobulnicky, H. A.; Sciortino, S.; Vink, J. S.

    2015-11-01

    We report on the analysis of the Chandra-ACIS data of O, B, and WR stars in the young association Cyg OB2. X-ray spectra of 49 O-stars, 54 B-stars, and 3 WR-stars are analyzed and for the brighter sources, the epoch dependence of the X-ray fluxes is investigated. The O-stars in Cyg OB2 follow a well-defined scaling relation between their X-ray and bolometric luminosities: {log}\\\\frac{{L}{{X}}}{{L}{bol}}=-7.2+/- 0.2. This relation is in excellent agreement with the one previously derived for the Carina OB1 association. Except for the brightest O-star binaries, there is no general X-ray overluminosity due to colliding winds in O-star binaries. Roughly half of the known B-stars in the surveyed field are detected, but they fail to display a clear relationship between LX and Lbol. Out of the three WR stars in Cyg OB2, probably only WR 144 is itself responsible for the observed level of X-ray emission, at a very low {log}\\\\frac{{L}{{X}}}{{L}{bol}}=-8.8+/- 0.2. The X-ray emission of the other two WR-stars (WR 145 and 146) is most probably due to their O-type companion along with a moderate contribution from a wind-wind interaction zone.

  20. THE PROTOPLANETARY DISKS IN THE NEARBY MASSIVE STAR-FORMING REGION CYGNUS OB2

    SciTech Connect

    Guarcello, M. G.; Drake, J. J.; Wright, N. J.; Hora, J. L.; Aldcroft, T.; Fruscione, A.; Kashyap, V. L.; Drew, J. E.; Gutermuth, R. A.; Naylor, T.; King, R.; Garcia-Alvarez, D.

    2013-08-20

    The formation of stars in massive clusters is one of the main modes of the star formation process. However, the study of massive star-forming regions is hampered by their typically large distances to the Sun. One exception to this is the massive star-forming region Cygnus OB2 in the Cygnus X region, at the distance of {approx}1400 pc. Cygnus OB2 hosts very rich populations of massive and low-mass stars, being the best target in our Galaxy to study the formation of stars, circumstellar disks, and planets in the presence of massive stars. In this paper, we combine a wide and deep set of photometric data, from the r band to 24 {mu}m, in order to select the disk-bearing population of stars in Cygnus OB2 and identify the class I, class II, and stars with transition and pre-transition disks. We selected 1843 sources with infrared excesses in an area of 1 Degree-Sign Multiplication-Sign 1 Degree-Sign centered on Cyg OB2 in several evolutionary stages: 8.4% class I, 13.1% flat-spectrum sources, 72.9% class II, 2.3% pre-transition disks, and 3.3% transition disks. The spatial distribution of these sources shows a central cluster surrounded by an annular overdensity and some clumps of recent star formation in the outer region. Several candidate subclusters are identified, both along the overdensity and in the rest of the association.

  1. QoS Provisioning for TT-TR Based OBS Ring Networks

    NASA Astrophysics Data System (ADS)

    Peng, Limei; Kim, Young-Chon; Tang, Wan; Youn, Chan-Hyun

    2011-06-01

    Optical burst switching (OBS) is one of the most promising switching technologies. This paper studies the efficient OBS ring network using the TT-TR based node architecture. The TT-TR based OBS ring network can use the network resources very efficiently, but the network performance is restricted due to the limited network resources. Moreover, different network demands have different network resource requirements. It is very important to satisfy their different requirements while using the limited network resources efficiently. In this paper, Quality of Service (QoS) provisioning mechanisms are studied to solve the above mentioned problems. Two kinds of network services, namely the real-time traffic and the best-effort traffic, are considered in this paper. The real-time service is delay-sensitive that should be transmitted in a real-time manner and cannot tolerate high transmission delay; while the best-effort traffic has no strict limitation in transmission delay or loss, but the best effort are made to improve their transmission reliability. According to their different servicing requirements, different QoS provisioning mechanisms are proposed to serve them in the OBS unidirectional and bidirectional ring networks, respectively. The proposed mechanisms are evaluated and analyzed through simulation. The simulation results show that even though the proposed mechanisms cannot get absolute zero-delay or zero-loss for real-time traffic or best-effort traffic, their different requirements for delay or loss are satisfied to some significant degree.

  2. SPECTROSCOPIC CONFIRMATION OF THE DRAGONFISH ASSOCIATION: THE GALAXY'S MOST LUMINOUS OB ASSOCIATION

    SciTech Connect

    Rahman, Mubdi; Moon, Dae-Sik; Matzner, Christopher D.

    2011-12-20

    Young OB associations with masses greater than 10{sup 4} M{sub Sun} have been inferred to exist in the Galaxy but have largely evaded detection. Recently, a candidate OB association has been identified within the most luminous star-forming complex in the Galaxy, the Dragonfish Nebula. We identify 18 young, massive stars with near-infrared spectroscopy from a sample of 50 members within the candidate OB association, including 15 O-type and 3 luminous blue variables or Wolf-Rayet stars. This number matches the expected yield of massive stars from the candidate association, confirming its existence and ability to power the parent star-forming complex. These results demonstrate the existence of a 10{sup 5} M{sub Sun} OB association, more powerful than any previously known in the Galaxy, comparable in mass only to Westerlund 1. Further, the results also validate the color selection method used to identify the association, adding credence to others discovered in the same way.

  3. TPP1 OB-fold domain controls telomere maintenance by recruiting telomerase to chromosome ends

    PubMed Central

    Zhong, Franklin L.; Batista, Luis F.Z.; Freund, Adam; Pech, Matthew F.; Venteicher, Andrew S.; Artandi, Steven E.

    2012-01-01

    Telomere synthesis in cancer cells and stem cells involves trafficking of telomerase to Cajal bodies, and telomerase is thought to be recruited to telomeres through interactions with telomere-binding proteins. Here, we show that the OB-fold domain of the telomere-binding protein TPP1 recruits telomerase to telomeres through an association with the telomerase reverse transcriptase, TERT. When tethered away from telomeres and other telomere-binding proteins, the TPP1 OB-fold domain is sufficient to recruit telomerase to a heterologous chromatin locus. A minimal TPP1 OB-fold serves to inhibit telomere maintenance by blocking access of telomerase to its cognate binding site at telomeres. We identify specific loop residues within the TPP1 OB-fold necessary for association with telomerase, and critical residues in TERT, including those mutated in a subset of pulmonary fibrosis patients. These data define a potential interface for telomerase-TPP1 interaction required for telomere maintenance and implicate defective telomerase recruitment in telomerase-related disease. PMID:22863003

  4. OBS seismic data preliminary results: Manila subduction zone (21°N)

    NASA Astrophysics Data System (ADS)

    Zhao, M.; Liu, S.; Sun, L.; Sibuet, J. C.; Zhang, J.; Chen, C.; Qiu, X.

    2016-12-01

    A two-dimensional ocean bottom seismometer (OBS) survey was performed in 2015, along an E-W trending line (OBS2015-2) located in front of the Manila subduction zone. The preliminary results show that OBS data are of high quality for the whole experiment. Seismic phases (such as Pg and PmP) are very clear in the OBSs' record sections. They are further identified by forward modeling using the Rayinvr software. The reflective seismic phases from the Moho interface (PmP) are observed in all OBS sections. The identification of these PmP phases used simultaneously with Fast and Tomo2D tomography results shows that the nature of the crust is essentially continental, not oceanic, except perhaps close to the trench. The obvious PmP phases in the incoming plate along the Manila Trench subduction zone, will further provide good constraints concerning the nature and evolution of the incoming plate in the Manila subduction zone. This research was granted by the Natural Science Foundation of China (91428204, 41176053) and the scientific cruise (NORC2015-8) by the R/V Shiyan 2.

  5. Ionizing Sources of Extragalactic HII Regions: Tight Clusters vs. Distributed OB Associations

    NASA Astrophysics Data System (ADS)

    Evans, Jessica Marie; Chu, Y.

    2011-01-01

    Extragalactic giant HII regions (EGHRs) are sites of active, concentrated star formation, providing ideal labs to analyze starburst phenomenon. The most interesting starburst issue is whether a tight cluster forms and later becomes a globular cluster or distributed OB associations form and later disperse. The two nearest EGHRs serve as standards for each; 30 Dor in the Large Magellanic Cloud hosts a dominant cluster and NGC 604 in M33 hosts multiple OB associations. To investigate the mode of star formation in EGHRs, we have searched the Hubble Legacy Archives (HLA) and identified a sample of >50 EGHRs in galaxies closer than 15 Mpc, limiting our selection by an H-alpha luminosity threshold of 1.5 x 1040 ergs s-1. Binned images of 30 Dor and NGC 604 in continuum bands are used to simulate clusters and distributed OB associations at distances of the EGHRs in our HLA sample. We have analyzed the continuum images of the EGHRs and compared them with the simulated images of 30 Dor and NGC 604 in order to assess whether the ionizing source is dominated by a tight cluster or distributed OB associations. We have also correlated the modes of star formation in EGHRs with the types of their host galaxies and their location within them. Implications of our results on starbursts and formation of EGHRs will be discussed.

  6. Degradation of trichloroethylene by methanol-growth cultures of Methylosinus trichosporium OB3b PP358

    SciTech Connect

    Fitch, M.W.; Georgiou, G.; Speitel, G.E. Jr.

    1996-03-01

    Methylosinus trichosporium OB3b is a methanotrophic bacteria which rapidly degrades chlorinated solvents including trichloroethylene. This report focuses on continuous growth of Methylosinus trichosporium PP358 and the influence of growth conditions on TCE degradation and transformation capacity. 20 refs., 2 tabs.

  7. Unified study of Quality of Service (QoS) in OPS/OBS networks

    NASA Astrophysics Data System (ADS)

    Hailu, Dawit Hadush; Lema, Gebrehiwet Gebrekrstos; Yekun, Ephrem Admasu; Kebede, Samrawit Haylu

    2017-07-01

    With the growth of Internet traffic, an inevitable use of optical networks provide a large bandwidth, fast data transmission rates and Quality of Service (QoS) support. Currently, Optical Burst Switched (OBS)/Optical Packet Switched (OPS) networks are under study as future solutions for addressing the increase demand of Internet traffic. However, due to their high blocking probability in the intermediate nodes they have been delayed in the industries. Packet loss in OBS/OPS networks is mainly occur due to contention. Hence, the contribution of this study is to analyze the file loss ratio (FLR), packet overhead and number of disjoint paths, and processing delay over Coded Packet Transport (CPT) scheme for OBS/OPS network using simulation. The simulations show that CPT scheme reduces the FLR in OBS/OPS network for the evaluated scenarios since the data packets are chopped off into blocks of the data packet for transmission over a network. Simulation results for secrecy and survivability are verified with the help of the analytical model to define the operational range of CPT scheme.

  8. AN R- AND I-BAND PHOTOMETRIC VARIABILITY SURVEY OF THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Henderson, C. B.; Stanek, K. Z.; Pejcha, O.; Prieto, J. L.

    2011-06-01

    We present a catalog of photometrically variable stars discovered within two 21.'3 x 21.'3 fields centered on the Cygnus OB2 association (Cyg OB2). There have hitherto been no deep optical variability studies of Cyg OB2, despite it being replete with early-type massive stars, likely due to the high and variable extinction (up to A{sub V} {approx} 20) that permeates much of the region. Here, we provide results of the first variability study with this combination of spatial coverage ({approx}0.5 deg) and photometric depth (R {approx} 21 mag). We find 121 stars to be variable in both R and I bands, 115 of them newly discovered. Of the 121 variables, we identify 27 eclipsing binaries and eclipsing-binary candidates, 52 pulsating variables, and 20 potential Herbig Ae/Be stars. Confirming both the status and the cluster membership of the Herbig Ae/Be stars would address the uncertainty regarding the age and star formation history of Cyg OB2. We match our catalog to known variables and binaries in the region, Two Micron All Sky Survey near-IR data, and Chandra X-ray observations to find counterparts to new variables in other wavelengths.

  9. How to Prepare Your Teen for That First Ob-Gyn Visit

    MedlinePlus

    ... finding a new one," Jacobstein said. "By being open and discussing the experience with your daughter, you can help to make the first visit to an ob-gyn a more comfortable one," she added. SOURCE: LifeBridge Health, news release, May 2017 HealthDay Copyright ( ...

  10. PREDICTING GAIA’S PARALLAX DISTANCE TO THE CYGNUS OB2 ASSOCIATION WITH ECLIPSING BINARIES

    SciTech Connect

    Kiminki, Daniel C.; Kobulnicky, Henry A.; Álvarez, Carlos A. Vargas; Alexander, Michael J.; Lundquist, Michael J.

    2015-10-01

    The Cygnus OB2 Association is one of the nearest and largest collections of massive stars in the Galaxy. Situated at the heart of the “Cygnus X” complex of star-forming regions and molecular clouds, its distance has proven elusive owing to the ambiguous nature of kinematic distances along this ℓ ≃ 80° sightline and the heavy, patchy extinction. In an effort to refine the three-dimensional geometry of key Cygnus X constituents, we have measured distances to four eclipsing double-lined OB-type spectroscopic binaries that are probable members of Cyg OB2. We find distances of 1.33 ± 0.17, 1.32 ± 0.07, 1.44 ± 0.18, and 1.32 ± 0.13 kpc toward MT91 372, MT91 696, CPR2002 A36, and Schulte 3, respectively. We adopt a weighted average distance of 1.33 ± 0.06 kpc. This agrees well with spectrophotometric estimates for the Association as a whole and with parallax measurements of protostellar masers in the surrounding interstellar clouds, thereby linking the ongoing star formation in these clouds with Cyg OB2. We also identify Schulte 3C (O9.5V), a 4″ visual companion to the 4.75 day binary Schulte 3(A+B), as a previously unrecognized Association member.

  11. Purification and characterization of oligonucleotide binding (OB)-fold protein from medicinal plant Tinospora cordifolia.

    PubMed

    Amir, Mohd; Haque, Md Anzarul; Wahiduzzaman; Dar, Mohammad Aasif; Islam, Asimul; Ahmad, Faizan; Hassan, Md Imtaiyaz

    2016-01-01

    The oligonucleotide binding fold (OB-fold) is a small structural motif present in many proteins. It is originally named for its oligonucleotide or oligosaccharide binding properties. These proteins have been identified as essential for replication, recombination and repair of DNA. We have successfully purified a protein contains OB-fold from the stem of Tinospora cordifolia, a medicinal plants of north India. Stems were crushed and centrifuged, and fraction obtained at 60% ammonium sulphate was extensively dialyzed and applied to the weak anion exchange chromatography on Hi-Trap DEAE-FF in 50mM Tris-HCl buffer at pH 8.0. Eluted fractions were concentrated and applied to gel filtration column to get pure protein. We observed a single band of 20-kDa on SDS-PAGE. Finally, the protein was identified as OB-fold by MALDI-TOF. The purified OB-fold protein was characterized for its secondary structural elements using circular dichroism (CD) in the far-UV region. Generally the OB-fold has a characteristic feature as five-stranded beta-sheet coiled to form a closed beta- barrel. To estimate its chemical stability, guanidinium chloride-induced denaturation curve was followed by observing changes in the far-UV CD as a function of the denaturant concentration. Analysis of this denaturation curve gave values of 8.90±0.25kcalmol(-1) and 3.78±0.18M for ΔGD° (Gibbs free energy change at 25°C) and Cm (midpoint of denaturation), respectively. To determine heat stability parameters of OB-fold protein, differential scanning calorimetry was performed. Calorimetric values of ΔGD°, Tm (midpoint of denaturation), ΔHm (enthalpy change at Tm), and ΔCp (constant-pressure heat capacity change) are 9.05±0.27kcalmol(-1), 85.2±0,3°C, 105±4kcalmol(-1) and 1.6±0.08kcalmol(-1)K(-1). This is the first report on the isolation, purification and characterization of OB-fold protein from a medicinal plant T. cordifolia.

  12. The deep OB star population in Carina from the VST Photometric Hα Survey (VPHAS+)

    NASA Astrophysics Data System (ADS)

    Mohr-Smith, M.; Drew, J. E.; Napiwotzki, R.; Simón-Díaz, S.; Wright, N. J.; Barentsen, G.; Eislöffel, J.; Farnhill, H. J.; Greimel, R.; Monguió, M.; Kalari, V.; Parker, Q. A.; Vink, J. S.

    2017-02-01

    Massive OB stars are critical to the ecology of galaxies and yet our knowledge of OB stars in the Milky Way, fainter than V ∼ 12, remains patchy. Data from the VST Photometric Hα Survey (VPHAS+) permit the construction of the first deep catalogues of blue excess-selected OB stars, without neglecting the stellar field. A total of 14 900 candidates with 2MASS cross-matches are blue-selected from a 42 deg2 region in the Galactic plane, capturing the Carina Arm over the Galactic longitude range 282° ≲ ℓ ≲ 293°. Spectral energy distribution fitting is performed on these candidates' combined VPHAS+ u, g, r, i and 2MASS J, H, K magnitudes. This delivers effective temperature constraints, statistically separating O from early-B stars and high-quality extinction parameters, A0 and RV (random errors typically ∼0.1). The high-confidence O-B2 candidates number 5915 and a further 5170 fit to later B spectral type. Spectroscopy of 276 of the former confirms 97 per cent of them. The fraction of emission-line stars among all candidate B stars is 7-8 per cent. Greyer (RV > 3.5) extinction laws are ubiquitous in the region, over the distance range 2.5-3 to ∼10 kpc. Near prominent massive clusters, RV tends to rise, with particularly large and chaotic excursions to RV ∼ 5 seen in the Carina Nebula. The data reveal a hitherto unnoticed association of 108 O-B2 stars around the O5If+ star LSS 2063 (ℓ = 289.77°, b = -1.22°). Treating the OB star scaleheight as a constant within the thin disc, we find an orderly mean relation between extinction (A0) and distance in the Galactic longitude range, 287.6° < ℓ < 293.5°, and infer the subtle onset of thin-disc warping. A halo around NGC 3603, roughly a degree in diameter, of ∼500 O-B2 stars with 4 < A0(mag) < 7 is noted.

  13. The HII Regions and OB Stars of M33 and NCG 6822.

    NASA Astrophysics Data System (ADS)

    Patel, Kanan

    1995-01-01

    We have used Hα and photometric data for two morphologically distinct Local Group galaxies, the spiral M33 and dwarf irregular NGC 6822, to study the distribution of the luminous blue O and B (OB) stars and HII regions in the galaxies as well as to determine whether individual regions of the galaxies are separately and/or collectively in a state of ionization balance. In the case of M33, we have concentrated on the inner 1 kpc region of the galaxy. Using the H alpha data, three distinct ionized gas environments (bright, halo and field) defined by the surface brightness of the Hα emission have been identified. We find that ~50% of the OB stars are located in the field, so that 1/2 of the lifetime of OB stars must be spent outside recognizable HII regions. We show that a possible origin for the large field OB population is that they were born in and subsequently percolated out of the ~10 ^3 giant molecular clouds with masses _sp{~}>10 ^3Modot predicted to exist within the inner kpc of the galaxy. Using UBV photometry and stellar ionization models, we predict Hα fluxes in the bright, halo and field regions. Our findings suggest that star formation rates obtained from luminosities must underestimate the true star formation rate within the inner region of M33. In the case of NGC 6822, four distinct components of the Hα emission (bright, halo, diffuse and field) differentiated by their surface brightnesses have been identified. We find that only 1/4 of the OB stars are found in the combined bright halo regions, suggesting that OB stars spend roughly 3/4 of their lifetimes outside "classical" HII regions. Molecular cloud lifetimes after forming OB stars could be as low as ~1 -3 times 10^6 yrs or 1/4 the typical main sequence lifetimes of OB stars if stars escape from bright HII regions by destroying their parent clouds. Additionally, the field population of OB stars cannot have originated in and percolated out of existing HII regions. Comparing the observed H

  14. Improving Depression Treatment for Women: Integrating a Collaborative Care Depression Intervention into OB-GYN Care

    PubMed Central

    LaRocco-Cockburn, Anna; Reed, Susan D.; Melville, Jennifer; Croicu, Carmen; Russo, Joan; Inspektor, Michal; Edmondson, Eddie; Katon, Wayne

    2013-01-01

    Background Women have higher rates of depression and often experience depression symptoms during critical reproductive periods, including adolescence, pregnancy, postpartum, and menopause. Collaborative care intervention models for mood disorders in patients receiving care in an OB-GYN clinic setting have not been evaluated. Study design and methodology for a randomized, controlled trial of collaborative care depression management versus usual care in OB-GYN clinics and the details of the adapted collaborative care intervention and model implementation are described in this paper. Methods Women over age 18 years with clinically significant symptoms of depression, as measured by a Patient Health Questionnaire-9 (PHQ-9) score ≥10 and a clinical diagnosis of major depression or dysthymia, were randomized to the study intervention or to usual care and were followed for 18 months. The primary outcome assessed was change over time in the SCL-20 depression scale between baseline and 12 months. Baseline Results 205 women were randomized: 57% white, 20% African American, 9% Asian or Pacific Islander, 7% Hispanic, and 6% Native American. Mean age was 39 years. 4.6% were pregnant and 7.5% were within 12 months postpartum. The majority were single, (52%), and 95% had at least the equivalent of a high school diploma. Almost all patients met DSM IV criteria for major depression (99%) and approximately 33% met criteria for dysthymia. Conclusions An OB-GYN collaborative care team including a social worker, psychiatrist and OB-GYN physician who met weekly and used an electronic tracking system for patients were essential elements of the proposed depression care treatment model described here. Further study of models that improve quality of depression care that are adapted to the unique OB-GYN setting are needed. PMID:23939510

  15. Radial velocities of a sample of ob stars in the Sco-Cen association

    NASA Astrophysics Data System (ADS)

    Jilinski, E.; de La Reza, R.; Cunha, K.

    2003-08-01

    Radial velocities (RV) were derived for a sample of OB stars belonging to Lower Centaurus Crux (LCC), Upper Centaurus Lupus (UCL) and Upper Scorpius (US) sub-groups of the Sco-Cen OB association. The knowledge of RV of OB stars of these sub-groups is important for membership determinations and the study of the past evolution and formation of these sub-groups. To measure RV of some stars belonging to the above mentioned sub-groups, 67 exposures of 56 stars were obtained between May 17, 2002 and July 7, 2002 with the 1.52 m ESO telescope equipped with FEROS echelle spectrograph (resolving power 48000 and spectral range 3550 - 9210 Å). RV were measured line by line. The cross-correlation technique is usually used for precise RV determinations of late type stars. Its application for the measurements of RV of OB stars can be problematical. On the one hand, spectra of early type stars show few absorption lines and these lines are intrinsically broad (up to a few hundreds km/sec). They are often broadened by stellar rotation and sometimes they also show variability. On the other hand, the cross-correlation peak is very broad and contains important sub-structures caused by the mixing of spectral lines of different widths. Moreover, a lot of OB stars are binaries and their broadened lines do not permit to observe them as double-lined binaries. The resulting mean values were compared with other observations in order to find new binary systems or to obtain more accurate RV for single stars. The values of the internal and external precisions of line by line measured RV are estimated as +/- 3.1 km/sec and +/- 5 km/sec. The results of these observations permitted us to recognize some stars (HD 120307, HD 116087, HD 139365 and HD 142990) as being probable spectral binaries due to their significant RV variations.

  16. Interstellar extinction toward the Cas OB6 association: Where is the dust?

    NASA Technical Reports Server (NTRS)

    Hanson, Margaret Murray; Clayton, Geoffrey C.

    1993-01-01

    We have completed a multiband (ultraviolet, optical, and near-infrared) study of the interstellar extinction properties of nine massive stars in IC 1805 and IC 1848, which are both part of Cas OB6 in the Perseus spiral arm. Our analysis includes determination of absolute extinction over the wavelength range from 3 micrometers to 1250 A. We have attempted to distinguish between foreground dust and dust local to Cas OB6. This is done by quantitatively comparing extinction laws of the least reddened sightlines (sampling mostly foreground dust) versus the most reddened sightlines (sampling a larger fraction of the dust in the Cas OB6 region). We have combined previous investigations to better understand the evolution of the interstellar medium in this active star forming region. We found no variation of extinction curve behavior between moderately reddend and heavily reddened Cas OB6 stars. None of the curves show any significant deviation from the Cardelli-Clayton-Mathis (CCM) R(sub upsilon)-dependent extinction. They are all consistent with that seen from diffuse dust. Most or all of the dust along the line of sight may be foreground to Cas OB6. Massive star forming regions can show significant deviations from CCM behavior which have been attributed to processing of the dust grains. Any dust local to the association must exist far from the hot stars in IC 1805 and IC 1848. A previous episode of star formation may have already cleared out the region of most of the gas and dust. Evidence for this can be seen in H I and IRAS data of the region.

  17. Effectiveness of a Labor Cervical Exam Model in Family Medicine and OB-GYN Residents.

    PubMed

    Nitsche, Joshua F; Fino, Nora F; Palomo, Jose M; Perdomo, Abraham Portillo; Brost, Brian C

    2017-05-01

    Labor cervical exam accuracy is an essential skill for family medicine and OB-GYN residents to master. To determine the effectiveness of simulation on labor cervical exam training, family medicine and OB-GYN residents were trained using a self-constructed PVC pipe-based cervical exam model during a short and intensive simulation workshop or "boot camp." A task trainer was constructed that allows for the blind examination of cervical dilation and effacement. This model was used in the training of first-year family medicine and OB-GYN residents during an 8 day simulation course. A longitudinal comparison of pre- and post-training accuracy was performed. Using a cohort design, the post-training accuracy of first-year family medicine and OB-GYN residents (interns) was also compared to second-fourth year OB-GYN residents. Use of the model by interns (n=25) resulted in significant improvements in the accuracy of their assessments of cervical dilation, but not effacement, and decreased intra-rater variability. When compared to the second-fourth year residents (n=25) who received traditional training, but not simulation training, interns were significantly more accurate and showed less intra-rater variability in their assessments of both dilation and effacement immediately after training compared to their senior colleagues. Training with the cervical exam model improved interns' accuracy and precision immediately after an 8-day simulation course. Use of this model in resident education may aid in the early stages of training and benefit more experienced trainees by augmenting traditional clinical training.

  18. Photoionization of the diffuse interstellar medium and galactic halo by OB associtations

    NASA Technical Reports Server (NTRS)

    Dove, James B.; Shull, J. Michael

    1994-01-01

    Assuming smoothly varying H I distributions in te Galactic disk, we have calculated the geometry of diffuse II regions due to OB associations in the Galactic plane. Near the solar circle, OB associations with a Lyman continuum (Lyc) photon luminosity Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1), produce H II regions that are density bounded in the vertical direction (H II chimneys) allowing Lyc to escape the gaseous disk and penetrate into the Galactic halo. We provide analytic formulae for the Lyc escape fraction as functions of S(sub 0) O-star catalog of Garmany and a new Lyc stellar stellar Lyc stellar flux calibration, we find a production rate of Lyc photons by OB associations within 2.5 kpc of Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1). Integrating the fraction of Lyc photons that escape the disk over our adopted luminosity function of OB associations, we estimate that approximately 7% of the ionizing photons, or Phi(sub Lyc) = 2.3 x 10(exp 6) cm(exp -2) s(exp -1), escape each side of the H I disk layer and penetrate the diffuse ionized medium ('Reynolds layer'). This flux is sufficient to explain the potoionization of this, although we have not constructed a model for the observed H-alpha emission and pulsar dispersion measures that is fully consistent with the absorption rate of Lyc in the H II layer. Since our quiescent model does not account for the effects of dynamic chimneys and superbubbles, which should enhance Lyc escape, we conclude the O stars are the probable source of ionizing radiation for the Reynolds layer. For a random distribution of OB associations throughout the disk, the Lyc flux is nearly uniform for heights Z is greater than approximately 0.8 kpc above the midplane.

  19. New OB star candidates in the Carina Arm around Westerlund 2 from VPHAS+

    NASA Astrophysics Data System (ADS)

    Mohr-Smith, M.; Drew, J. E.; Barentsen, G.; Wright, N. J.; Napiwotzki, R.; Corradi, R. L. M.; Eislöffel, J.; Groot, P.; Kalari, V.; Parker, Q. A.; Raddi, R.; Sale, S. E.; Unruh, Y. C.; Vink, J. S.; Wesson, R.

    2015-07-01

    O and early B stars are at the apex of galactic ecology, but in the Milky Way, only a minority of them may yet have been identified. We present the results of a pilot study to select and parametrize OB star candidates in the Southern Galactic plane, down to a limiting magnitude of g = 20. A 2 deg2 field capturing the Carina Arm around the young massive star cluster, Westerlund 2, is examined. The confirmed OB stars in this cluster are used to validate our identification method, based on selection from the (u - g, g - r) diagram for the region. Our Markov Chain Monte Carlo fitting method combines VPHAS+ u, g, r, i with published J, H, K photometry in order to derive posterior probability distributions of the stellar parameters log (Teff) and distance modulus, together with the reddening parameters A0 and RV. The stellar parameters are sufficient to confirm OB status while the reddening parameters are determined to a precision of σ(A0) ˜ 0.09 and σ(RV) ˜ 0.08. There are 489 objects that fit well as new OB candidates, earlier than ˜B2. This total includes 74 probable massive O stars, 5 likely blue supergiants and 32 reddened subdwarfs. This increases the number of previously known and candidate OB stars in the region by nearly a factor of 10. Most of the new objects are likely to be at distances between 3 and 6 kpc. We have confirmed the results of previous studies that, at these longer distances, these sight lines require non-standard reddening laws with 3.5 < RV < 4.

  20. ObsPy: A Python toolbox for seismology - Sustainability, New Features, and Applications

    NASA Astrophysics Data System (ADS)

    Krischer, L.; Megies, T.; Sales de Andrade, E.; Barsch, R.; MacCarthy, J.

    2016-12-01

    ObsPy (https://www.obspy.org) is a community-driven, open-source project dedicated to offer a bridge for seismology into the scientific Python ecosystem. Amongst other things, it provides Read and write support for essentially every commonly used data format in seismology with a unified interface. This includes waveform data as well as station and event meta information. A signal processing toolbox tuned to the specific needs of seismologists. Integrated access to the largest data centers, web services, and databases. Wrappers around third party codes like libmseed and evalresp. Using ObsPy enables users to take advantage of the vast scientific ecosystem that has developed around Python. In contrast to many other programming languages and tools, Python is simple enough to enable an exploratory and interactive coding style desired by many scientists. At the same time it is a full-fledged programming language usable by software engineers to build complex and large programs. This combination makes it very suitable for use in seismology where research code often must be translated to stable and production ready environments, especially in the age of big data. ObsPy has seen constant development for more than six years and enjoys a large rate of adoption in the seismological community with thousands of users. Successful applications include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. Additionally it sparked the development of several more specialized packages slowly building a modern seismological ecosystem around it. We will present a short overview of the capabilities of ObsPy and point out several representative use cases and more specialized software built around ObsPy. Additionally we will discuss new and upcoming features, as well as the sustainability of open-source scientific software.

  1. Improving depression treatment for women: integrating a collaborative care depression intervention into OB-GYN care.

    PubMed

    LaRocco-Cockburn, Anna; Reed, Susan D; Melville, Jennifer; Croicu, Carmen; Russo, Joan E; Inspektor, Michal; Edmondson, Eddie; Katon, Wayne

    2013-11-01

    Women have higher rates of depression and often experience depression symptoms during critical reproductive periods, including adolescence, pregnancy, postpartum, and menopause. Collaborative care intervention models for mood disorders in patients receiving care in an OB-GYN clinic setting have not been evaluated. Study design and methodology for a randomized controlled trial of collaborative care depression management versus usual care in OB-GYN clinics and the details of the adapted collaborative care intervention and model implementation are described in this paper. Women over age 18 years with clinically significant symptoms of depression, as measured by a Patient Health Questionnaire-9 (PHQ-9) score ≥10 and a clinical diagnosis of major depression or dysthymia, were randomized to the study intervention or to usual care and were followed for 18 months. The primary outcome assessed was change over time in the SCL-20 depression scale between baseline and 12 months. Two hundred five women were randomized: 57% white, 20% African American, 9% Asian or Pacific Islander, 7% Hispanic, and 6% Native American. Mean age was 39 years. 4.6% were pregnant and 7.5% were within 12 months postpartum. The majority were single (52%), and 95% had at least the equivalent of a high school diploma. Almost all patients met DSM IV criteria for major depression (99%) and approximately 33% met criteria for dysthymia. An OB-GYN collaborative care team, including a social worker, a psychiatrist, and an OB-GYN physician, who met weekly and used an electronic tracking system for patients was the essential element of the proposed depression care treatment model described here. Further study of models that improve quality of depression care that are adapted to the unique OB-GYN setting is needed. © 2013 Elsevier Inc. All rights reserved.

  2. ObsPy: A Python Toolbox for Seismology and Seismological Observatories

    NASA Astrophysics Data System (ADS)

    Krischer, Lion; Megies, Tobias; Barsch, Robert; Beyreuther, Moritz; Wassermann, Joachim

    2013-04-01

    Python combines the power of a full-blown programming language with the flexibility and accessibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. ObsPy extends Python's capabilities to fit the specific needs that arise when working with seismological data. It a) comes with a continuously growing signal processing toolbox that covers most tasks common in seismological analysis, b) provides read and write support for many common waveform, station and event metadata formats and c) enables access to various data centers, webservices and databases to retrieve waveform data and station/event metadata. In combination with mature and free Python packages like NumPy, SciPy, Matplotlib, IPython and PyQt, ObsPy makes it possible to develop complete workflows in Python, ranging from reading locally stored data or requesting data from one or more different data centers via signal analysis and data processing to visualization in GUI and web applications, output of modified/derived data and the creation of publication-quality figures. All functionality is extensively documented and the ObsPy Tutorial and Gallery give a good impression of the wide range of possible use cases. ObsPy is tested and running on Linux, OS X and Windows and comes with installation routines for these systems. ObsPy is developed in a test-driven approach and is available under the GPL/LGPLv3 open source licences. Users are welcome to request help, report bugs, propose enhancements or contribute code via either the user mailing list or the project page on GitHub.

  3. Structure-Function Analysis of the OB and Latch Domains of Chlorella Virus DNA Ligase*

    PubMed Central

    Samai, Poulami; Shuman, Stewart

    2011-01-01

    Chlorella virus DNA ligase (ChVLig) is a minimized eukaryal ATP-dependent DNA sealing enzyme with an intrinsic nick-sensing function. ChVLig consists of three structural domains, nucleotidyltransferase (NTase), OB-fold, and latch, that envelop the nicked DNA as a C-shaped protein clamp. The OB domain engages the DNA minor groove on the face of the duplex behind the nick, and it makes contacts to amino acids in the NTase domain surrounding the ligase active site. The latch module occupies the DNA major groove flanking the nick. Residues at the tip of the latch contact the NTase domain to close the ligase clamp. Here we performed a structure-guided mutational analysis of the OB and latch domains. Alanine scanning defined seven individual amino acids as essential in vivo (Lys-274, Arg-285, Phe-286, and Val-288 in the OB domain; Asn-214, Phe-215, and Tyr-217 in the latch), after which structure-activity relations were clarified by conservative substitutions. Biochemical tests of the composite nick sealing reaction and of each of the three chemical steps of the ligation pathway highlighted the importance of Arg-285 and Phe-286 in the catalysis of the DNA adenylylation and phosphodiester synthesis reactions. Phe-286 interacts with the nick 5′-phosphate nucleotide and the 3′-OH base pair and distorts the DNA helical conformation at the nick. Arg-285 is a key component of the OB-NTase interface, where it forms a salt bridge to the essential Asp-29 side chain, which is imputed to coordinate divalent metal catalysts during the nick sealing steps. PMID:21527793

  4. ObsPy: A Python Toolbox for Seismology - Recent Developments and Applications

    NASA Astrophysics Data System (ADS)

    Megies, T.; Krischer, L.; Barsch, R.; Sales de Andrade, E.; Beyreuther, M.

    2014-12-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project dedicated to building a bridge for seismology into the scientific Python ecosystem. It offersa) read and write support for essentially all commonly used waveform, station, and event metadata file formats with a unified interface,b) a comprehensive signal processing toolbox tuned to the needs of seismologists,c) integrated access to all large data centers, web services and databases, andd) convenient wrappers to legacy codes like libtau and evalresp.Python, currently the most popular language for teaching introductory computer science courses at top-ranked U.S. departments, is a full-blown programming language with the flexibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. Together with packages like NumPy, SciPy, Matplotlib, IPython, Pandas, lxml, and PyQt, ObsPy enables the construction of complete workflows in Python. These vary from reading locally stored data or requesting data from one or more different data centers through to signal analysis and data processing and on to visualizations in GUI and web applications, output of modified/derived data and the creation of publication-quality figures.ObsPy enjoys a large world-wide rate of adoption in the community. Applications successfully using it include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. All functionality is extensively documented and the ObsPy tutorial and gallery give a good impression of the wide range of possible use cases.We will present the basic features of ObsPy, new developments and applications, and a roadmap for the near future and discuss the sustainability of our open-source development model.

  5. Effects of an acute bout of exercise on serum soluble leptin receptor (sOB-R) levels.

    PubMed

    Yang, Chung Bing; Chuang, Chung-Chief; Kuo, Chung Sen; Hsu, Chin Hsing; Tsao, Te Hung

    2014-01-01

    We investigated the effects of an acute bout of exercise on serum soluble leptin receptor (sOB-R) concentrations. Eighteen male participants completed two different exercise sessions with intensities of 25% and 65% maximal aerobic capacity (VO2max). In addition to the energy expenditure during exercise sessions being measured, blood samples were collected before exercise, and immediately, at 24 h, and at 48 h post-exercise to analyse sOB-R, leptin and insulin levels. At 24 h post-exercise, sOB-R and leptin concentrations at the 65% VO2max were significantly different from those at the 25% VO2max. Leptin levels at 48 h post-exercise were also significantly lower for the 65% VO2max than for the 25% VO2max (P < 0.01). In the 65% VO2max session, the energy expenditure during exercise was significantly associated with leptin concentrations at 24 h and 48 h and sOB-R concentrations at 24 h post-exercise. However, no correlations were found between sOB-R and leptin at the three post-exercise time points. In conclusion, an acute bout of exercise with 920 kcal of output resulted in an increase in sOB-R levels at 24 h post-exercise. However, the changes in sOB-R levels due to an acute bout of exercise might not contribute to the delayed decrease observed for leptin.

  6. Immunoneutralization of endogenous glucagon reduces hepatic glucose output and improves long-term glycemic control in diabetic ob/ob mice.

    PubMed

    Sørensen, Heidi; Brand, Christian L; Neschen, Susanne; Holst, Jens Juul; Fosgerau, Keld; Nishimura, Erica; Shulman, Gerald I

    2006-10-01

    In type 2 diabetes, glucagon levels are elevated in relation to the prevailing insulin and glucose levels. The relative hyperglucagonemia is linked to increased hepatic glucose output (HGO) and hyperglycemia. Antagonizing the effects of glucagon is therefore considered an attractive target for treatment of type 2 diabetes. In the current study, effects of eliminating glucagon signaling with a glucagon monoclonal antibody (mAb) were investigated in the diabetic ob/ob mouse. Acute effects of inhibiting glucagon action were studied by an oral glucose tolerance test (OGTT) and by measurement of HGO. In addition, the effects of subchronic (5 and 14 days) glucagon mAb treatment on plasma glucose, insulin, triglycerides, and HbA1c (A1C) levels were investigated. Glucagon mAb treatment reduced the area under the curve for glucose after an OGTT, reduced HGO, and increased the rate of hepatic glycogen synthesis. Glucagon mAb treatment for 5 days lowered plasma glucose and triglyceride levels, whereas 14 days of glucagon mAb treatment reduced A1C. In conclusion, acute and subchronic neutralization of endogenous glucagon improves glycemic control, thus supporting the contention that glucagon antagonism may represent a beneficial treatment of diabetes.

  7. Marked augmentation of PLGA nanoparticle-induced metabolically beneficial impact of γ-oryzanol on fuel dyshomeostasis in genetically obese-diabetic ob/ob mice.

    PubMed

    Kozuka, Chisayo; Shimizu-Okabe, Chigusa; Takayama, Chitoshi; Nakano, Kaku; Morinaga, Hidetaka; Kinjo, Ayano; Fukuda, Kotaro; Kamei, Asuka; Yasuoka, Akihito; Kondo, Takashi; Abe, Keiko; Egashira, Kensuke; Masuzaki, Hiroaki

    2017-11-01

    Our previous works demonstrated that brown rice-specific bioactive substance, γ-oryzanol acts as a chaperone, attenuates exaggerated endoplasmic reticulum (ER) stress in brain hypothalamus and pancreatic islets, thereby ameliorating metabolic derangement in high fat diet (HFD)-induced obese diabetic mice. However, extremely low absorption efficiency from intestine of γ-oryzanol is a tough obstacle for the clinical application. Therefore, in this study, to overcome extremely low bioavailability of γ-oryzanol with super-high lipophilicity, we encapsulated γ-oryzanol in polymer poly (DL-lactide-co-glycolide) (PLGA) nanoparticles (Nano-Orz), and evaluated its metabolically beneficial impact in genetically obese-diabetic ob/ob mice, the best-known severest diabetic model in mice. To our surprise, Nano-Orz markedly ameliorated fuel metabolism with an unexpected magnitude (∼1000-fold lower dose) compared with regular γ-oryzanol. Furthermore, such a conspicuous impact was achievable by its administration once every 2 weeks. Besides the excellent impact on dysfunction of hypothalamus and pancreatic islets, Nano-Orz markedly decreased ER stress and inflammation in liver and adipose tissue. Collectively, nanotechnology-based developments of functional foods oriented toward γ-oryzanol shed light on the novel approach for the treatment of a variety of metabolic diseases in humans.

  8. Carnosic acid attenuates obesity-induced glucose intolerance and hepatic fat accumulation by modulating genes of lipid metabolism in C57BL/6J-ob/ob mice.

    PubMed

    Park, Mi-Young; Sung, Mi-Kyung

    2015-03-15

    Carnosic acid (CA), a major bioactive component of rosemary (Rosmarinus officinalis) leaves, is known to possess antioxidant and anti-adipogenic activities. In this study it was hypothesized that CA would ameliorate obesity-induced glucose intolerence and hepatic fat accumulation, and possible mechanisms are suggested. It was observed that a 0.02% (w/w) CA diet effectively decreased body weight, liver weight and blood triglyceride (TG) and total cholesterol levels (P < 0.05) compared with the control diet. CA at 0.02% significantly improved glucose tolerance, and hepatic TG accumulation was reduced in a dose-dependent manner. Hepatic lipogenic-related gene (L-FABP, SCD1 and FAS) expression decreased whereas lipolysis-related gene (CPT1) expression increased in animals fed the 0.02% CA diet (P < 0.05). Long-chain fatty acid content and the ratio of C18:1/C18:0 fatty acids were decreased in adipose tissue of animals fed the 0.02% CA diet (P < 0.05). Serum inflammatory mediators were also decreased significantly in animals fed the 0.02% CA diet compared with those of the obese control group (P < 0.05). These results suggest that CA is an effective anti-obesity agent that regulates fatty acid metabolism in C57BL/6J-ob/ob mice. © 2014 Society of Chemical Industry.

  9. Possible Detection of Pi(exp 0)-Decay Gamma-Ray Emission from Cyg OB2 by EGRET

    NASA Technical Reports Server (NTRS)

    Chen, W.; White, R. L.; Bertsch, D.

    1996-01-01

    We report possible detection of pi (exp 0) decay radiation from Cyg OB2, a nearby (1.7 kpc) massive OB star association. The EGRET flux (greater than 100 MeV) maps clearly indicate a point source whose error circle includes both Cyg OB2 and Cyg X-3. We show that Cyg X-3 is unlikely to be the counterpart for the EGRET source, because of the marginal spatial consistency and the lack of the 4.8 hour modulation seen in other high energy emissions from Cyg X-3. If confirmed, this will be the first detection by EGRET of massive stars.

  10. Red spectra of the brightest stars in Cygnus OB2 - Possible detection of two new interstellar bands

    NASA Astrophysics Data System (ADS)

    Torres-Dodgen, Ana V.

    1990-12-01

    The stellar and interstellar spectra of the 12 brightest stars in the Cygnus OB2 association are described. The observations were made in the wavelength range 5800 A-8500 A, at about 7 A resolution. Two new lines, centered near 7430 A and 7932 A, may be previously undetected interstellar bands. The former may be formed in the interstellar medium as well as in the dust environment of Cyg OB2, while the latter may be formed only in the dust environment of Cyg OB2.

  11. A Business Case Analysis (BCA) of the One Box - One Wire (OB1) Joint Combined Technology Demonstration (JCTD)

    DTIC Science & Technology

    2009-03-01

    BLDG A - SECRET Rei ana Below " Internet G Main Distribution Type-1 Frame ( MDF )Encryptor ••CIPR OB1 - SwitchNIPR Router B .,....UNCLASS Type-1 Router...Encryptor G OB1-PC G Switch Router Router Coalition Server SECRET RELFarm COALITION Enclave BLDG B - SECRET ana Below Router " MDF Switch " G B Server...Server Switch Below EnclaveFarm Router US Only SCIF BLDG C - TS/SCI and Below Switch Server Rm MDF ., OB1 - Switch G Router B ~~~~~torG " B" JWICS G Server

  12. Proposal of a multi-layer network architecture for OBS/GMPLS network interworking

    NASA Astrophysics Data System (ADS)

    Guo, Hongxiang; Tsuritani, Takehiro; Yin, Yawei; Otani, Tomohiro; Wu, Jian

    2007-11-01

    In order to enable the existing optical circuit switching (OCS) network to support both wavelength and subwavelength granularities, this paper proposes overlay-based multi-layer network architecture for interworking the generalized multi-protocol label switching (GMPLS) controlled OCS network with optical burst switching (OBS) networks. A dedicated GMPLS border controller with necessary GMPLS extensions, including group label switching path (LSP) provisioning, node capability advertisement, and standard wavelength label as well as wavelength availability advertisement, is introduced in this multi-layer network to enable a simple but flexible interworking operation. The feasibility of this proposal is experimentally confirmed by demonstrating an OBS/GMPLS testbed, in which the extended node capability advertisement and group LSP functions successfully enabled the burst header packet (BHP) and data burst (DB) to transmit over a GMPLS-controlled transparent OCS network.

  13. BOREAS TF-3 Automated Chamber CO2 Flux Data from the NSA-OBS

    NASA Technical Reports Server (NTRS)

    Goulden, Michael L.; Crill, Patrick M.; Hall, Forrest G. (Editor); Conrad, Sara (Editor)

    2000-01-01

    The BOReal Ecosystem Atmosphere Study Tower Flux (BOREAS TF-3) and Trace Gas Biogeochemistry (TGB-1) teams collected automated CO2 chamber flux data in their efforts to fully describe the CO2 flux at the Northern Study Area-Old Black Spruce (NSA-OBS) site. This data set contains fluxes of CO2 at the NSA-OBS site measured using automated chambers. In addition to reporting the CO2 flux, it reports chamber air temperature, moss temperature, and light levels during each measurement. The data set covers the period from 23-Sep-1995 through 26-Oct-1995 and from 28-May-1996 through 21-Oct-1996. The data are stored in tabular ASCII files.

  14. Discharges and yields of suspended sediment in the Ob' and Yenisey Rivers of Siberia

    USGS Publications Warehouse

    Bobrovitskaya, N.N.; Zubkova, C.; Meade, R.H.

    1996-01-01

    The northward-flowing rivers of Siberia deliver immense quantities of water but only relatively small quantities of sediment to the Arctic Ocean. The relatively low delivery of sediment to the ocean by these rivers is explained by the large areas of forest and swamp in their basins. In the Ob' River, sediment yields tend to increase between the headwaters and Kamen' na Obi. Further downstream, sediment yields gradually decrease. Near the mouth of the Ob' River, at Salekhard, sediment yield has remained constant at about 5.3 t km-2 year-1. In the lower Yenisey River, the already small natural sediment yield of 5.4 t km-2 year-1 has been decreased several fold by the construction of massive reservoirs on the main stem and on the tributary Angara River, and presently equals 1.8 t km-2 year-1.

  15. Organic carbon flow in the Ob, Yenisey Rivers and Kara Sea of the Arctic region.

    PubMed

    Krishnamurthy, R V; Machavaram, M; Baskaran, M; Brooks, J M; Champs, M A

    2001-09-01

    Stable carbon isotope and elemental C/N ratios of the organic fraction of a set of samples along a transect in the Ob and Yenisey Rivers into the Kara Sea in the Arctic were measured. Previously, the concentrations of 239,240Pu and 137Cs in these same samples had been determined. The coupled measurements were carried out to assess possible connectivity between organic carbon flow into the Kara Sea and transport of radioactive nuclides in this marine environment. Organic carbon flow into the Kara Sea is influenced significantly by terrigenous sources carried by the Ob and Yenisey Rivers. The carbon isotope-organic carbon relationship provides evidence that a rich source of terrigenous carbon exists in the riverine system. A weak, but significant relationship between stable carbon isotope ratio and 137Cs suggests that most of the 137Cs is derived from riverine particles, as compared to Pu which is also derived from in situ scavenging within the water column.

  16. BOREAS TGB-8 Photosynthetic Rate Data over the SSA-OBS and the SSA-OJP

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Conrad, Sara K. (Editor); Lerdau, Manuel

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set includes measurements of photosynthetic rates at mature jack pine and black spruce sites. The data were collected at the OJP and OBS tower flux locations in the BOREAS SSA. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  17. Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst.

    PubMed

    Hwang, In Yeub; Hur, Dong Hoon; Lee, Jae Hoon; Park, Chang-Ho; Chang, In Seop; Lee, Jin Won; Lee, Eun Yeol

    2015-03-01

    Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30°C. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.

  18. Mobile Health Apps in OB-GYN-Embedded Psychiatric Care: Commentary.

    PubMed

    Mehralizade, Aydan; Schor, Shayna; Coleman, Chad M; Oppenheim, Claire E; Denckla, Christy A; Borba, Christina Pc; Henderson, David C; Wolff, James; Crane, Sarah; Nettles-Gomez, Pamela; Pal, Avik; Milanovic, Snezana

    2017-10-06

    This paper explores the potential benefits of the use of mobile health (mHealth) apps in obstetrician-gynecologist (OB-GYN)-embedded psychiatric clinics in the United States. First, we highlight the increasing trend of integrating mental health care within the OB-GYN context. Second, we provide examples of successful uses of mHealth in the global health context and highlight the dearth of available research in the United States. Finally, we provide a summary of the shortcomings of currently available apps and describe the upcoming trial of a novel app currently underway at the Mother-Child Wellness Clinical and Research Center at Boston Medical Center. ©Aydan Mehralizade, Shayna Schor, Chad M Coleman, Claire E Oppenheim, Christy A Denckla, Christina PC Borba, David C Henderson, James Wolff, Sarah Crane, Pamela Nettles-Gomez, Avik Pal, Snezana Milanovic. Originally published in JMIR Mhealth and Uhealth (http://mhealth.jmir.org), 06.10.2017.

  19. The Copper Chelator Methanobactin from Methylosinus Trichosporium OB3b Binds Copper(I)

    SciTech Connect

    Hakemian,A.; Tinberg, C.; Kondapali, K.; Tesler, J.; Hoffman, B.; Stemmler, T.; Rosenzweig, A.

    2005-01-01

    The oxidation state of copper bound to methanobactin, a small siderophore-like molecule from the methanotroph Methylosinus trichosporium OB3b, was investigated. Purified methanobactin loaded with Cu(II) exhibits a weak EPR signal probably due to adventitious Cu(II). The EPR signal intensity increases significantly upon addition of the strong oxidant nitric acid. Features of the X-ray absorption near edge spectrum, including a 1s {yields} 4p transition at 8985 eV, further indicate the presence of Cu(I). EXAFS data were best fit using a multiple scattering model generated from previously reported crystallographic parameters. These results establish definitively that M. trichosporium OB3b methanobactin binds Cu(I) and suggest that methanobactin itself reduces Cu(II) to Cu(I).

  20. A burst segmentation-deflection routing contention resolution mechanism in OBS networks

    NASA Astrophysics Data System (ADS)

    Guan, Ai-hong; Wang, Bo-yun

    2012-01-01

    One of the key problems to hinder the realization of optical burst switching (OBS) technology in the core networks is the losses due to the contention among the bursts at the core nodes. Burst segmentation is an effective contention resolution technique used to reduce the number of packets lost due to the burst losses. In our work, a burst segmentation-deflection routing contention resolution mechanism in OBS networks is proposed. When the contention occurs, the bursts are segmented according to the lowest packet loss probability of networks firstly, and then the segmented burst is deflected on the optimum routing. An analytical model is proposed to evaluate the contention resolution mechanism. Simulation results show that high-priority bursts have significantly lower packet loss probability and transmission delay than the low-priority. And the performance of the burst lengths, in which the number of segments per burst distributes geometrically, is more effective than that of the deterministically distributed burst lengths.

  1. Ara OB1 - A stellar association formed by the action of an energetic event?

    NASA Technical Reports Server (NTRS)

    Arnal, E. M.; Cersosimo, J. C.; May, J.; Bronfman, L.

    1987-01-01

    Neutral hydrogen (at the wavelength of 21 cm) and carbon monoxide (at 2.6 mm) line observations of the interstellar medium in the neighborhood of the association Ara OB1 are reported. The observed H I distribution indicates the presence of an expanding structure. The total H I mass associated with such feature amounts to 4800 solar masses, and its momentum and kinetic energy are about 40,000 solar mass km/s and 3 x 10 to the 48th erg, respectively. The H I structure, 42 pc in diameter, expands at a speed of 10 km/s. A possible origin for the expanding H I structure, and a genetic link between such structure and Ara OB1 are proposed.

  2. Molecular Clouds Toward a New OB Association in Pup-CMa

    NASA Astrophysics Data System (ADS)

    May, J.; Gyulbudaghian, A. L.; Alvarez, H.

    2005-10-01

    We have mapped 16 molecular clouds toward a new OB association in the Pup-CMa region to derive their physical properties. The observations were carried out in the 12CO (J = 1 0) line with the Southern millimetre-wave Telescope at Cerro Tololo, Chile. Distances have been determined kinematically using the rotation curve of Brand with R⊙ = 8.5 kpc and V⊙ = 220 km/s. Masses have been derived adopting a CO luminosity to H2 conversion factor X = 3.8 . 1020 molecules cm-2 (K km/s)-1. The observed mean radial velocity of the clouds is comparable with the mean radial velocity of stars composing an OB association in Pup-CMa; it is in favor of the close connection of clouds with these stars.

  3. OB-Rb gene transfer to leptin-resistant islets reverses diabetogenic phenotype

    PubMed Central

    Wang, May-Yun; Koyama, Kazunori; Shimabukuro, Michio; Newgard, Christopher B.; Unger, Roger H.

    1998-01-01

    In obese Zucker diabetic fatty (ZDF) rats with mutant leptin receptors, pancreatic islets have an ≈50-fold increase in fat (TG), overproduce nitric oxide (NO), and lack a normal proinsulin mRNA response to fatty acids. We overexpressed the wild-type full-length “b” isoform of the leptin receptor (OB-Rb) in ZDF islets by perfusing ZDF pancreata with recombinant adenovirus containing the cDNA encoding OB-Rb. In cultured islets isolated from these animals, leptin lowered islet TG by 87% and completely blocked TG formation from free fatty acids. Overproduction of NO was reduced, and the preproinsulin mRNA response to free fatty acids was restored. This establishes defective leptin action as the proximate cause of lipotoxic diabetes in ZDF rats. PMID:9435258

  4. On the Nature of Anomalous Reddening of the Hypergiant Cygnus OB2 #12

    NASA Astrophysics Data System (ADS)

    Maryeva, O. V.; Chentsov, E. L.; Goranskij, V. P.; Dyachenko, V. V.; Karpov, S. V.

    2017-06-01

    To explain the nature of the anomalously high reddening (AV≃ 10 mag) towards one of the most luminous stars in the Galaxy - Cyg OB2 #12 (B5 Ia-0), also known as MT304, we carried out spectro-photometric observations of 24 stars located in its vicinity. We included five new B-stars to the members of Cygnus OB2, and for five more photometrically selected stars we spectroscopically confirmed their membership. We constructed the map of interstellar extinction within 2'.5 radius and found that interstellar extinction increases towards MT304. The increase of reddening suggests that the reddening excess may be caused by the circumstellar shell ejected by the star during its evolution. We also report the detection of a second component of MT304, and discovery of an even fainter third component, based on data of speckle interferometric observations taken with the Russian 6-m telescope.

  5. ARA OB1 - A stellar association formed by the action of an energetic event?

    NASA Astrophysics Data System (ADS)

    Arnal, E. M.; Cersosimo, J. C.; May, J.; Bronfman, L.

    1987-03-01

    Neutral hydrogen (at λ = 21 cm) and carbon monoxide (at λ = 2.6 mm) line observations of the interstellar medium in the neighbourhood of the association Ara OB1 are reported. The observed H I distribution indicates the presence of an expanding structure. The total H I mass associated with such feature amounts to 4800 solar masses, and its momentum and kinetic energy are ≡4×104M_sun; km s-1 and 3×1048erg, respectively. The H I structure, 42 pc in diameter, expands at a speed of 10 km s-1. A possible origin for the expanding H I structure, and a genetic link between such structure and Ara OB1 are proposed.

  6. ObStruct: a method to objectively analyse factors driving population structure using Bayesian ancestry profiles.

    PubMed

    Gayevskiy, Velimir; Klaere, Steffen; Knight, Sarah; Goddard, Matthew R

    2014-01-01

    Bayesian inference methods are extensively used to detect the presence of population structure given genetic data. The primary output of software implementing these methods are ancestry profiles of sampled individuals. While these profiles robustly partition the data into subgroups, currently there is no objective method to determine whether the fixed factor of interest (e.g. geographic origin) correlates with inferred subgroups or not, and if so, which populations are driving this correlation. We present ObStruct, a novel tool to objectively analyse the nature of structure revealed in Bayesian ancestry profiles using established statistical methods. ObStruct evaluates the extent of structural similarity between sampled and inferred populations, tests the significance of population differentiation, provides information on the contribution of sampled and inferred populations to the observed structure and crucially determines whether the predetermined factor of interest correlates with inferred population structure. Analyses of simulated and experimental data highlight ObStruct's ability to objectively assess the nature of structure in populations. We show the method is capable of capturing an increase in the level of structure with increasing time since divergence between simulated populations. Further, we applied the method to a highly structured dataset of 1,484 humans from seven continents and a less structured dataset of 179 Saccharomyces cerevisiae from three regions in New Zealand. Our results show that ObStruct provides an objective metric to classify the degree, drivers and significance of inferred structure, as well as providing novel insights into the relationships between sampled populations, and adds a final step to the pipeline for population structure analyses.

  7. Signals embedded in the OBS records, in light of Gabor Spectral Analysis

    NASA Astrophysics Data System (ADS)

    Chang, T.; Wang, Y.; Chang, C.; Lee, C.

    2005-12-01

    Since the last decades, seismological survey has been expanded to marine area, with goal of making up the deficiency of seismogenic study outside the land. Although teleseismic data can resolve plate boundaries location and certain seismic parameters for great earthquake, local seismogenic frame can be merely emerged by the seismic network in situ. The Ocean Bottom Seismometer (OBS), therefore, is developing for this kind of purpose and becoming an important facility for seismological study. This work introduces a synthesized spectral method to analyze the seismograms recorded by 15 OBS deployed at the Okinawa trough in 14 days (Nov. 19 ~Dec. 2, 2003). Geological background of Okinawa trough is well known to correspond with the back-arc spreading in the regime of the Philippine Sea plate subducting northward beneath the Eurasia plate. As the complex affections at sea bottom, for instance, strong current, slope slumping, turbidite flow, and even sea animal attack, the OBS seismogram show a rather noisy sequence in comparison with the record on land. However, hundreds of tectonic earthquake can be extracted from such noisy records (done by Drs. Lin and Sibuet). Our job is to sort out the signals with the distinguishable sources by means of a systematically spectral analysis. The continuous wavelet transform and short-term Fourier transform, both taking Gaussian function as kernel, are synthesized as the Gabor transform in data process. The use of a limited Gaussian window along time axis with negligible low frequency error can largely enhance the stability of discrete Fourier spectrum. With a proper window factor selection, the Gabor transform can improve the resolution of spectrogram in time domain. We have converted the OBS records into spectrograms to detect the variation of signal causes. Up-to-date, some tremors signals and strong current oscillations have been told apart from these continuous records with varied frequency composing. We anticipate the further

  8. Escape fraction of ionizing photons during reionization: Effects due to supernova feedback and runaway ob stars

    SciTech Connect

    Kimm, Taysun; Cen, Renyue

    2014-06-20

    The fraction of hydrogen ionizing photons escaping from galaxies into the intergalactic medium is a critical ingredient in the theory of reionization. We use two zoomed-in, high-resolution (4 pc), cosmological radiation hydrodynamic simulations with adaptive mesh refinement to investigate the impact of two physical mechanisms (supernova, SN, feedback, and runaway OB stars) on the escape fraction (f {sub esc}) at the epoch of reionization (z ≥ 7). We implement a new, physically motivated SN feedback model that can approximate the Sedov solutions at all (from the free expansion to snowplow) stages. We find that there is a significant time delay of about ten million years between the peak of star formation and that of escape fraction, due to the time required for the build-up and subsequent destruction of the star-forming cloud by SN feedback. Consequently, the photon number-weighted mean escape fraction for dwarf galaxies in halos of mass 10{sup 8}-10{sup 10.5} M {sub ☉} is found to be 〈f{sub esc}〉∼11%, although instantaneous values of f {sub esc} > 20% are common when star formation is strongly modulated by the SN explosions. We find that the inclusion of runaway OB stars increases the mean escape fraction by 22% to 〈f{sub esc}〉∼14%. As SNe resulting from runaway OB stars tend to occur in less dense environments, the feedback effect is enhanced and star formation is further suppressed in halos with M{sub vir}≳10{sup 9} M{sub ⊙} in the simulation with runaway OB stars compared with the model without them. While both our models produce enough ionizing photons to maintain a fully ionized universe at z ≤ 7 as observed, a still higher amount of ionizing photons at z ≥ 9 appears necessary to accommodate the high observed electron optical depth inferred from cosmic microwave background observations.

  9. Communities of bacteria and viruses in waters of the Gulf of Ob and Taz Estuary.

    PubMed

    Pavlova, M A; Makarevich, P R; Shirokolobova, T I

    2016-11-01

    The study of the most abundant components in freshwater plankton in the Gulf of Ob and Taz Estuary in the summer-autumn season has demonstrated that the abundance and biomass of bacteria are stable and typical for mesotrophic waters during active microalgae vegetation. The abundance of viral particles varies in the range which is reported for unproductive or medium-productive water bodies. The environmental factors affecting affect the development and patterns of bacterio- and virioplankton distribution are considered.

  10. A Mathematical Model for the Reduction Stage of the CAS-OB Process

    NASA Astrophysics Data System (ADS)

    Sulasalmi, Petri; Visuri, Ville-Valtteri; Kärnä, Aki; Järvinen, Mika; Ollila, Seppo; Fabritius, Timo

    2016-12-01

    This paper proposes a novel method for modeling the reduction stage of the CAS-OB process (composition adjustment by sealed argon bubbling-oxygen blowing). Our previous study proposed a model for the heating stage of the CAS-OB process; the purpose of the present study is to extend this work toward a more comprehensive model for the process in question. The CAS-OB process is designed for homogenization and control of the composition and temperature of steel. During the reduction stage, the steel phase is stirred intensely by employing the gas nozzles at the bottom of the ladle, which blow argon gas. It is assumed that the reduction rate of the top slag is dictated by the formation of slag droplets at the steel-slag interface. Slag droplets, which are generated due to turning of the steel flow in the spout, contribute mainly by increasing the interfacial area between the steel and slag phases. This phenomenon has been taken into account based on our previous study, in which the droplet size distribution and generation rate at different steel flow velocities. The reactions considered between the slag and steel phases are assumed to be mass transfer controlled and reversible. We validated the results from the model against the measurements from the real CAS-OB process. The results indicate that the model accurately predicts the end compositions of slag and steel. Moreover, it was discovered that the cooling rate of steel during the gas stirring given by the model is consistent with the results reported in the literature.

  11. Alma Observations Of Circumstellar Disks In The Upper Scorpius Ob Association

    NASA Astrophysics Data System (ADS)

    Barenfeld, Scott

    2016-07-01

    We present ALMA observations of 106 G-, K-, and M-type stars in the Upper Scorpius OB Association hosting circumstellar disks. With these data, we measure the total dust masses of these disks. We find that disk dust masses in Upper Scorpius are a factor of 4.5 times lower than those in the younger Taurus star forming region, clear evidence of disk evolution between 1-2 Myr and 5-10 Myr.

  12. The large scale view of the young stellar populations in the Orion OB1 Association

    NASA Astrophysics Data System (ADS)

    Briceno, Cesar; Calvet, Nuria

    2016-06-01

    The Orion OB1 association, at ~400 pc and with a wide range of ages (~1-10 Myr) and environmental conditions, is an ideal place to look at how stars form, first evolve and disperse among the general population of field stars. Also to study disk dispersal and the duration of the planet formation phase.However, despite spanning nearly 200 deg2 on the sky, almost all we know about Orion comes from studies of a limited fraction of the entire region, mostly of the youngest objects (~<1 Myr) in the A and B molecular clouds and the ~3 Myr old sigma Ori cluster.We will present here the results of our 180 sq deg photometric multi-epoch survey across the Orion OB1 association, using the known variability of T Tauri stars to pick them among the general field population, and following with spectroscopy to confirm members and characterize them.The ~2000 newly identified young low-mass stars are mostly located away from the molecular clouds, across tens of sq. deg. in the Orion OB1a and OB1b sub-associations, with ages in the range ~4-10 Myr. But within this general population we identify a significant fraction concentrated in distinct overdensities, most notably the ~7 Myr old 25 Orionis cluster. These stellar aggregates point to a previously unknown degree of substructure that has survived the dissipation of the parent molecular clouds. We also find that the Orion Nebula Cluster is surrounded by a few sq.deg. halo of young stars, as has been suggested by recent sudies.

  13. VizieR Online Data Catalog: Deep OB star population in Carina (Mohr-Smith+, 2017)

    NASA Astrophysics Data System (ADS)

    Mohr-Smith, M.; Drew, J. E.; Napiwotzki, R.; Simon-Diaz, S.; Wright, N. J.; Barentsen, G.; Eisloeffel, J.; Farnhill, H. J.; Greimel, R.; Monguio, M.; Kalari, V.; Parker, Q. A.; Vink, J. S.

    2016-11-01

    The sky positions and photometric measurements from VPHAS+ (and 2MASS) for 14900 candidate OB stars blue-selected from the Carina region of the Galactic plane are set out in the first 24 columns. The optical magnitudes are expressed in the Vega system. These are followed by the parameters and their error estimates obtained from main-sequence fits to the optical/NIR magnitudes, along with the chi-squared measure of goodness of fit (columns 25 to 33). For the 3 degrees of freedom involved, chi-squared values up to 7.82 may be regarded as 'good fits': 5915 objects are good fits in this sense and have estimated effective temperatures high enough to place them into the O-B2 range. The fitting process delivers effective temperatures, (highly uncertain) distance moduli, and the extinction parameters, A0 and RV. Subsequent columns (34 to 37) identify which candidates are: good O-B2 fits; likely to be emission line stars (based on r-Halpha excess); candidate sub-luminous stars; candidate higher-luminosity evolved objects. The 'Notes' in column 38 provide information on cross-identifications to known objects, where these exist. Columns 39 to 49 present spectroscopically-derived parameters and associated errors for the subset of 276 stars with spectra. (1 data file).

  14. Galactic OB associations in the northern Milky Way Galaxy. I - Longitudes 55 deg to 150 deg

    NASA Technical Reports Server (NTRS)

    Garmany, C. D.; Stencel, R. E.

    1992-01-01

    The literature on all OB associations was reviewed, and their IRAS point source content was studied, between galactic longitude 55 and 150 deg. Only one third of the 24 associations listed by Ruprecht et al. (1981) have been the subject of individual studies designed to identify the brightest stars. Distances to all of these were recomputed using the method of cluster fitting of the B main sequence stars, which makes it poossible to reexamine the absolute magnitude calibration of the O stars, as well as for the red supergiant candidate stars. Also examined was the composite HR diagram for these associations. Associations with the best defined main sequences, which also tend to contain very young clusters, referred to here as OB clusters, have extremely few evolved B and A or red supergiants. Associations with poorly defined main sequences and few OB clusters have many more evolved stars. They also show an effect in the upper HR diagram referred to as a ledge by Fitzpatrick and Garmany (1990) in similar data for the Large Magellanic Cloud. It is suggested that the differences in the associations are not just observational selection effects but represent real differences in age and formation history.

  15. B fields in OB stars (BOB): Concluding the FORS 2 observing campaign

    NASA Astrophysics Data System (ADS)

    Schöller, M.; Hubrig, S.; Fossati, L.; Carroll, T. A.; Briquet, M.; Oskinova, L. M.; Järvinen, S.; Ilyin, I.; Castro, N.; Morel, T.; Langer, N.; Przybilla, N.; Nieva, M.-F.; Kholtygin, A. F.; Sana, H.; Herrero, A.; Barbá, R. H.; de Koter, A.; BOB Collaboration

    2017-03-01

    Aims: The B fields in OB stars (BOB) Collaboration is based on an ESO Large Programme to study the occurrence rate, properties, and ultimately the origin of magnetic fields in massive stars. Methods: In the framework of this program, we carried out low-resolution spectropolarimetric observations of a large sample of massive stars using FORS 2 installed at the ESO VLT 8 m telescope. Results: We determined the magnetic field values with two completely independent reduction and analysis pipelines. Our in-depth study of the magnetic field measurements shows that differences between our two pipelines are usually well within 3σ errors. From the 32 observations of 28 OB stars, we were able to monitor the magnetic fields in CPD -57° 3509 and HD 164492C, confirm the magnetic field in HD 54879, and detect a magnetic field in CPD -62° 2124. We obtain a magnetic field detection rate of 6 ± 3% for the full sample of 69 OB stars observed with FORS 2 within the BOB program. For the preselected objects with a v sin i below 60 km s-1, we obtain a magnetic field detection rate of 5 ± 5%. We also discuss X-ray properties and multiplicity of the objects in our FORS 2 sample with respect to the magnetic field detections. Based on observations made with ESO Telescopes at the La Silla Paranal Observatory under program ID 191.D-0255(E,G).

  16. Large Scale Variability Surveys from Venezuela: Orion OB1 and beyond

    NASA Astrophysics Data System (ADS)

    Briceño, C.; Calvet, N.; Vivas, A. K.; Hartmann, L.

    We present our scheme and initial results for variability surveys spanning hundreds of square degrees near the celestial equator, carried out with an 8k x 8k CCD Mosaic Camera optimized for drift-scanning, installed on the 1m Schmidt telescope at the Venezuela National Astronomical Observatory. In the Orion OB1 association, one of the nearest and most active regions of star formation, we are conducting a 120 sqr.deg. VRIHalpha survey to map the low mass young stellar population in this region. The absence of dust and gas around the young stars in the ˜ 10 Myr Ori OB 1a sub-association suggests that star formation is a rapid process, and that molecular clouds do not last more than a few million years after the first stars are born. The lack of accretion indicators or near IR emission from inner dusty disks among stars in Ori OB 1a suggests that significant disk dissipation has occurred in a few Myr, possibly due to the coagulation/agglomeration of dust particles into larger bodies like planetesimals or planets. The results of our variability surveys will be made available through a massive database equipped with web-based data mining tools, as part of the effort leading to the International Virtual Observatory.

  17. Imaging beneath an opaque basaltic layer using densely sampled wide-angle OBS data

    SciTech Connect

    Samson, C.; Barton, P.J.; Karwatowski, J.

    1995-05-01

    A combined reflection/refraction (wide-angle) seismic survey was conducted on the continental shelf north-west of Britain, using a conventional streamer with an air gun source, and static ocean-bottom seismometers (OBS) to record wide-angle energy. The shallow structure down to a basaltic layer was reasonably well imaged on the stacked reflection section. The basalts, however, proved to be opaque to the conventional reflection method and prevented the imaging of deeper horizons, where an important velocity inversion was anticipated. This paper reports on the processing, modeling and interpretation of the densely sampled wide-angle OBS data that were coincident with the reflection profile. Eleven OBS instruments were deployed along a 75 km line and recorded signal from a powerful 149 liter (9100 in.{sup 3}) air gun array fired every 50 m. Data processing was performed using a standard industrial reflection seismic software package prior to first-arrival picking. Processing steps included geometry definition, trace summation and display of the data using various scaling algorithms. An initial model was constructed from 1D velocity-time profiles digitized every 4 km along the stacked section. First arrival travel time modeling rapidly converged to a detailed model of the structure of the top 5 km of the crust. Modeling revealed the existence of a buried low-velocity Mesozoic sedimentary basin, of a prominent basement horst and of a normal fault penetrating to the basement.

  18. CLUSTERING BETWEEN HIGH-MASS X-RAY BINARIES AND OB ASSOCIATIONS IN THE MILKY WAY

    SciTech Connect

    Bodaghee, A.; Tomsick, J. A.; Rodriguez, J.

    2012-01-10

    We present the first direct measurement of the spatial cross-correlation function of high-mass X-ray binaries (HMXBs) and active OB star-forming complexes in the Milky Way. This result relied on a sample containing 79 hard X-ray-selected HMXBs and 458 OB associations. Clustering between the two populations is detected with a significance above 7{sigma} for distances <1 kpc. Thus, HMXBs closely trace the underlying distribution of the massive star-forming regions that are expected to produce the progenitor stars of HMXBs. The average offset of 0.4 {+-} 0.2 kpc between HMXBs and OB associations is consistent with being due to natal kicks at velocities of the order of 100 {+-} 50 km s{sup -1}. The characteristic scale of the correlation function suggests an average kinematical age (since the supernova phase) of {approx}4 Myr for the HMXB population. Despite being derived from a global view of our Galaxy, these signatures of HMXB evolution are consistent with theoretical expectations as well as observations of individual objects.

  19. VizieR Online Data Catalog: OB stars in Milky Way (Lynga, 1964)

    NASA Astrophysics Data System (ADS)

    Lynga, G.

    2009-10-01

    The list below shows precise coordinates and IDs for the southern OB stars found by Lynga (1964MeLu2.141....1L). In general I relied on Lynga's CPD or CD identification to identify the stars. It was usually obvious when these were incorrect, either by star-color or motion, or the fact that it was not included in any later surveys (per the Reed OB stars catalogue, V/125). I used Lynga's charts if available to identify the stars in 2002, and re-examined some of these for the present revision. All the CD names from Lyngas catalog where checked -- the italic font used for CD numbers in his printed list is not always distinctive. Lynga missed a few CD/CPD identifications. Coordinates are now from UCAC2 or Tycho-2. I show complete HD, CD, and CPD names, since the latter two are often very similar and are mixed-up in the literature as well as in the source list. I give approximate V magnitudes either from published photometry (via VizieR items II/168, II/215, or V/125) or from ASAS-3 (http://www.astrouw.edu.pl/asas/?page=aasc). The Reed OB stars catalogue lacks magnitudes for many of the fainter stars. (1 data file).

  20. A model for the infrared emission from an OB star cluster environment

    NASA Technical Reports Server (NTRS)

    Leisawitz, D.

    1990-01-01

    Researchers developed an interactive radiative transfer code that predicts the infrared emission from an HII region containing diffuse ionized and atomic gas and dense molecular clouds. This model complements the investigation of the redistribution of OB star luminosity in the interstellar medium (Leisawitz and Hauser 1988, Ap. J., 332, 954). The model can be used as a diagnostic tool to probe the radiation field and matter density in an HII region, place constraints on the proximity and orientation of an illuminated molecular cloud with respect to the ionizing stars, test for the presence of small, transiently heated dust grains, and determine whether the dust-to-gas ratio is normal. Predictions of the model agree qualitatively and quantitatively with observations of blister-type HII regions ionized by well-studied OB clusters in which the distribution of dense neutral material is known. This is illustrated by a model for Infrared Astronomy Satellite (IRAS) observations of the region around NGC 7380 (S142). Researchers plan to use the model in a survey of regions of massive star formation in the outer Galaxy to study OB stars embedded to various degrees in their parental molecular clouds.

  1. On the nature of high reddening of Cygnus OB2 #12 hypergiant

    NASA Astrophysics Data System (ADS)

    Maryeva, O. V.; Chentsov, E. L.; Goranskij, V. P.; Dyachenko, V. V.; Karpov, S. V.; Malogolovets, E. V.; Rastegaev, D. A.

    2016-05-01

    To explain the nature of the high reddening (AV ≃ 10 mag) towards one of the most luminous stars in the Galaxy - Cyg OB2 #12 (B5 Ia-0), also known as MT304, we carried out spectrophotometric observations of 24 stars located in its vicinity. We included five new B-stars among the members of Cygnus OB2, and for five more photometrically selected stars we spectroscopically confirmed their membership. We constructed the map of interstellar extinction within 2.5 arcmin radius and found that interstellar extinction increases towards MT304. According to our results the most reddened OB-stars in the association after MT304 are J203240.35+411420.1 and J203239.90+411436.2, located about 15 arcsec away from it. Interstellar extinction towards these stars is about 9 mag. The increase of reddening towards MT304 suggests that the reddening excess may be caused by the circumstellar shell ejected by the star during its evolution. This shell absorbs 1 mag, but its chemical composition and temperature are unclear. We also report the detection of a second component of MT304, and discovery of an even fainter third component, based on data of speckle interferometric observations taken with the Russian 6-m telescope.

  2. Human obesity reduces the number of hepatic leptin receptor (ob-R) expressing NK cells.

    PubMed

    Lautenbach, Anne; Breitmeier, Dirk; Kuhlmann, Susanne; Nave, Heike

    2011-01-01

    In the industrialized world, obesity is an increasing socioeconomic health problem. Obese subjects have a higher risk of developing several types of cancer. NK cells are an integral component of the innate immune system, able to destruct tumor cells. The adipokine leptin plays a crucial role in the development of obesity and its related diseases. Peripheral leptin signaling is modulated by the liver. The aim of this study was to evaluate the number of hepatic NK cells (CD56+) and the number of leptin-receptor positive (Ob-R+) cells in the livers of five normal-weight and five obese humans. Livers were removed during autopsy and accurately defined sections were stained immunohistochemically and CD56+, Ob-R+, and double-positive cells were quantified. Results revealed a dramatic reduction of NK cells and Ob-R-expressing NK cells in the livers of obese individuals. The present study demonstrates, for the first time, body-weight-dependent numbers of hepatic NK cells. This supports the hypothesis of obesity-associated alterations of immune cell numbers in different human organs.

  3. Obesity epidemic: how to make a difference in a busy OB/GYN practice.

    PubMed

    Shaw, Kate A; Caughey, Aaron B; Edelman, Alison B

    2012-06-01

    At just one-third of the American population, those with a normal body mass index are now in the minority in the United States, whereas 68% are overweight or obese. The key to reducing the prevalence of obesity and improving the health of our population is, of course, screening and prevention. Screening (as simple as a weight and height) is effective, inexpensive, and already part of the routine vital signs taken at every visit. However, providers often avoid tackling the issue of weight due to a misperception that treatment is not effective, or from fear of causing offense or compromising rapport. However, clearly more harm is done by not discussing this important health issue. Cardiovascular disease remains the number 1 killer of women, and obesity is the leading modifiable risk factor. Beyond heart disease, obesity has implications for every visit type seen in the OB/GYN office, from contraception to pregnancy to abnormal bleeding to cancer. In addition, maternal obesity adversely affects future generations, making the impact of obesity a never-ending cycle. OB/GYNs are often the only physicians that reproductive-aged women see, and, thus, OB/GYNs have the opportunity to provide a potentially life-altering intervention. Effective treatment is available and includes lifestyle changes, behavioral counseling, medication, and bariatric surgery. Time is always a limitation in a busy practice but becoming more comfortable with how to approach patients, the language to use and tailoring counseling can save time increase impact.

  4. High Resolution Spectroscopy of C_2 and CN in the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    McCall, Benjamin J.; Oka, Takeshi

    2000-08-01

    The unexpected detection of a large column density of hhh along the lines of sight to Cygnus OB2 #12 and Cygnus OB2 #5 cannot be explained by the standard models of diffuse cloud chemistry, which imply unreasonably long absorption path lengths (hundreds of parsecs). In order to gather more information about the physical condition of the diffuse gas in these lines of sight, we propose to obtain high resolution (R 120 000) visible spectra of several stars in the Cygnus OB2 association, including #12 and #5. The observed rotational distribution of the diatomics çand CN will enable us to estimate the kinetic temperature and number density of the molecular gas. In addition, the high resolution of the HRS at HET will allow us to study the velocity distribution of both the atomic (K I) and molecular (çand CN) gas along these lines of sight. Together with our previous observations of hhh, the temperatures, number densities, and velocity distributions from the proposed observations will seriously constrain theoretical models of these sightlines, such as that recently proposed by Cecchi-Pestellini and Dalgarno.

  5. Diacylglycerol production induced by growth hormone in Ob1771 preadipocytes arises from phosphatidylcholine breakdown

    SciTech Connect

    Catalioto, R.M.; Ailhaud, G.; Negrel, R. )

    1990-12-31

    Growth Hormone has recently been shown to stimulate the formation of diacylglycerol in Ob1771 mouse preadipocyte cells without increasing inositol lipid turnover. Addition of growth hormone to Ob1771 cells prelabelled with ({sup 3}H)glycerol or ({sup 3}H)choline led to a rapid, transient and stoechiometric formation of labelled diacylglycerol and phosphocholine, respectively. In contrast, no change was observed in the level of choline and phosphatidic acid whereas the release of water-soluble metabolites in ({sup 3}H)ethanolamine prelabelled cells exposed to growth hormone was hardly detectable. Stimulation by growth hormone of cells prelabelled with (2-palmitoyl 9, 10 ({sup 3}H))phosphatidylcholine also induced the production of labelled diacyglycerol. Pertussis toxin abolished both diacylglycerol and phosphocholine formation induced by growth hormone. It is concluded that growth hormone mediates diacylglycerol production in Ob1771 cells by means of phosphatidylcholine breakdown involving a phospholipase C which is likely coupled to the growth hormone receptor via a pertussis toxin-sensitive G-protein.

  6. Photometric Survey of Intermediate Mass Members of the Orion OB1 Association

    NASA Astrophysics Data System (ADS)

    Sherry, William; Walter, Frederick; Brittain, Sean; Kim, Jinyoung Serena

    2009-08-01

    We propose to survey 15 deg^2 of the OB1a and OB1b groups of the Orion OB1 association to measure UBVRI photometry of high and intermediate mass members of the association. This survey will address two goals. First, it will extend the photometric coverage of association members to cover the full spectrum of masses between 0.01 M_⊙ and 40 M_⊙ - extant photometry misses most stars in the range 1 M_⊙ to 3 M_⊙ (10

  7. CHARACTERIZATION OF THE YOUNG OPEN CLUSTER G144.9+0.4 IN THE CAMELOPARDALIS OB1 ASSOCIATION

    SciTech Connect

    Lin, Chien-Cheng; Chen, W. P.; Panwar, Neelam

    2013-10-01

    Our star-count analysis of the Two Micron All Sky Survey point sources resulted in an identification of the star cluster G144.9+0.4. The cluster was found, but not characterized, by Glushkova et al. We show that the cluster is physically associated with the Cam OB1 association at a distance of about 1 kpc and with an age of 1-2 Myr. Pre-main sequence stars are identified on the basis of photometric and proper motion data. A total of 91 additional OB star candidates was found in subgroups 1A and 1B, a significant increase from the currently known 43 OB stars. The OB members show an age spread that indicates a sustained star formation for at least the last 10-15 Myr. The young cluster G144.9+0.4 represents the latest episode of sequential star formation in this cloud complex.

  8. 25 Orionis: A Kinematically Distinct 10 Myr Old Group in Orion OB1a

    NASA Astrophysics Data System (ADS)

    Briceño, César; Hartmann, Lee; Hernández, Jesús; Calvet, Nuria; Vivas, A. Katherina; Furesz, Gabor; Szentgyorgyi, Andrew

    2007-06-01

    We report here on the photometric and kinematic properties of a well-defined group of nearly 200 low-mass pre-main-sequence stars, concentrated within ~1° of the early-B star 25 Ori, in the Orion OB1a subassociation. We refer to this stellar aggregate as the 25 Orionis group. The group also harbors the Herbig Ae/Be star V346 Ori and a dozen other early-type stars with photometry, parallaxes, and some with IR excess emission, indicative of group membership. The number of high- and low-mass stars is in agreement with expectations from a standard initial mass function. The velocity distribution for the low-mass stars shows a narrow peak at 19.7 km s-1, offset ~-10 km s-1 from the velocity characterizing the younger stars of the Ori OB1b subassociation, and -4 km s-1 from the velocity of widely spread young stars of the Ori OB1a population; this result provides new and compelling evidence that the 25 Ori group is a distinct kinematic entity, and that considerable space and velocity structure is present in the Ori OB1a subassociation. The low-mass members follow a well-defined band in the color-magnitude diagram, consistent with an isochronal age of ~7-10 Myr. The ~2 time drop in the overall Li I equivalent widths and accretion fraction between the younger Ori OB1b and the 25 Ori group is consistent with the latter being significantly older. In a simple-minded kinematic evolution scenario, the 25 Ori group may represent the evolved counterpart of the younger σ Ori cluster. The 25 Ori stellar aggregate is the most populous ~10 Myr sample yet known within 500 pc, setting it as an excellent laboratory to study the evolution of solar-like stars and protoplanetary disks. Based on observations obtained at the Llano del Hato National Astronomical Observatory of Venezuela, operated by CIDA for the Ministerio de Ciencia y Tecnología the MMT Observatory, a joint facility of the Smithsonian Institution and the University of Arizona; and the Fred Lawrence Whipple Observatory of

  9. OB stars at the lowest Local Group metallicity. GTC-OSIRIS observations of Sextans A

    NASA Astrophysics Data System (ADS)

    Camacho, I.; Garcia, M.; Herrero, A.; Simón-Díaz, S.

    2016-01-01

    Context. Massive stars play an important role in the chemical and dynamical evolution of the Universe. The first metal-poor stars may have started the reionization of the Universe. To understand these early epochs it is necessary to know the behavior and the physical properties of massive stars in very metal-poor environments. We focus on the massive stellar content of the metal-poor irregular galaxy Sextans A. Aims: Our aim is to find and classify OB stars in Sextans A, so as to later determine accurate stellar parameters of these blue massive stars in this low-metallicity region (Z ~ 0.1 Z⊙). Methods: Using UBV photometry, the reddening-free index Q and GALEX imaging, we built a list of blue massive star candidates in Sextans A. We obtained low-resolution (R ~ 1000) GTC-OSIRIS spectra for a fraction of them and carried out spectral classification. For the confirmed O-stars, we derived preliminary stellar parameters. Results: The target selection criteria and observations were successful and have produced the first spectroscopic atlas of OB-type stars in Sextans A. From the whole sample of 18 observed stars, 12 were classified as early OB-types, including 5 O-stars. The radial velocities of all target stars are in agreement with their Sextans A membership, although three of them show significant deviations. We determined the stellar parameters of the O-type stars using the stellar atmosphere code FASTWIND and revisited the sub-SMC temperature scale. Two of the O-stars are consistent with relatively strong winds and enhanced helium abundances, although results are not conclusive. We discuss the position of the OB stars in the HRD. Initial stellar masses run from slightly below 20 up to 40 solar masses. Conclusions: The target selection method worked well for Sextans A. The stellar temperatures are consistent with findings in other galaxies. Some of the targets deserve follow-up spectroscopy because of indications of a runaway nature, an enhanced helium abundance

  10. OB Runaway Stars inside the Supernova Remnants S147 and IC443

    NASA Astrophysics Data System (ADS)

    Dincel, B.; Neuhauser, R.; Yerli, S. K.; Ankay, A.; Pannicke, A.; Sasaki, M.

    2016-06-01

    We present first results of a long term study: Searching for OB-type runaway stars inside supernova remnants (SNRs). We identified spectral types and measured radial velocities (RV) by optical spectroscopic observations and we found OB runaway stars inside SNR S147 and IC443. HD 37424 is a B0.5V type star with a peculiar velocity of 74.0 +/- 8 km/s. Tracing back the past trajectories via Monte Carlo simulations, we found that HD 37424 was located at the same position as the central source PSR J0538+2817 30 +/- 4 kyr ago. This position is only ~4 arcmin away from the geometrical center of the SNR. So, we suggest that HD 37424 was the pre-supernova binary companion to the progenitor of the pulsar and the SNR. We found a distance of 1333+103-112 pc to the SNR. The age is 30 +/- 4 kyr and the total visual extinction towards the center is 1.28 +/-0.06 mag. The zero age main sequence progenitor mass should be greater than 13 Solar Masses. We calculated the pre--supernova binary parameters for different progenitor masses. The values found for the Roche Lobe radii suggest that it was an interacting binary in the late stages of the progenitor. This is the first OB runaway star ever found which is directly linked to a neutron star (NS) and a SNR. Another OB runaway star we found is the B0II/III type star HD 254577 inside SNR IC443. The proper motion of the star is significantly larger than the average proper motion of the other members of GEM OB1 association. The peculiar velocity of the star is 35 +/- 7 km/s at 1.5 kpc. The bow shock direction of the pulsar wind nebula shows that the NS and HD 254577 may have a common origin; binary supernova disruption. Unlike S147, the explosion center we found is far away from the geometrical center, close to the eastern edge of the remnant. But the relation to the SNR is still possible. This source provided us with important information of SNR expansion in the medium with inhomogeneous density distribution.

  11. MUG-OBS - Multiparameter Geophysical Ocean Bottom System : a new instrumental approach to monitor earthquakes.

    NASA Astrophysics Data System (ADS)

    Hello, Y.; Yegikyan, M.; Charvis, P.; Verfaillie, R.; Philippe, O.

    2015-12-01

    There are several attempts to monitor real time seismic activity, using regional scale wired nodes, such as Neptune in Canada and in the U.S, Antares in France or DONET in Japan.On another hand there are also initiatives in deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight 6-months deployments) and the Osisec program in Ecuador (four 6-months deployments). These OBSs are autonomous, they are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data.Among the most recent alternative we developed a 3-years autonomy OBS equipped with a Nanometrics Trillium 120 s, a triaxial accelerometer, a differential, an absolute pressure gauge, and a hydrophone. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a seismic crisis) utilizing one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years (when an OBS is redeployed on the same site, it will not land in the same place), it is a perfect tool to monitor slow seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. A new version is being developed; for remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two- way Iridium

  12. A M2FS Spectroscopic Study of Low-mass Young Stars in Orion OB1

    NASA Astrophysics Data System (ADS)

    Kaleida, Catherine C.; Briceno, Cesar; Calvet, Nuria; Mateo, Mario L.; Hernandez, Jesus

    2015-01-01

    Surveys of pre-main sequence stars in the ~4-10 Myr range provide a window into the decline of the accretion phase of stars and the formation of planets. Nearby star clusters and stellar associations allow for the study of these young stellar populations all the way down to the lowest mass members. One of the best examples of nearby 4-10 Myr old stellar populations is the Orion OB1 association. The CIDA Variability Survey of Orion OB1 (CVSO - Briceño et al. 2001) has used the variability properties of low-mass pre-main-sequence (PMS) stars to identify hundreds of K and M-type stellar members of the Orion OB1 association, a number of them displaying IR-excess emission and thought to be representative of more evolved disk-bearing young stars. Characterizing these young, low-mass objects using spectroscopy is integral to understanding the accretion phase in young stars. We present preliminary results of a spectroscopic survey of candidate and confirmed Orion OB1 low-mass members taken during November 2014 and February 2014 using the Michigan/Magellan Fiber Spectrograph (M2FS), a PI instrument on the Magellan Clay Telescope (PI: M. Matteo). Target fields located in the off-cloud regions of Orion were identified in the CVSO, and observed using the low and high-resolution modes of M2FS. Both low and high-resolution spectra are needed in order to confirm membership and derive masses, ages, kinematics and accretion properties. Initial analysis of these spectra reveal many new K and M-type members of the Orion OB1 association in these low extinction, off-cloud areas. These are the more evolved siblings of the youngest stars still embedded in the molecular clouds, like those in the Orion Nebula Cluster. With membership and spectroscopic indicators of accretion we are building the most comprehensive stellar census of this association, enabling us to derive a robust estimate of the fraction of young stars still accreting at a various ages, a key constraint for the end of

  13. Integration of a permanent OBS offshore NE Iberian Peninsula to the Catalan Seismic Network

    NASA Astrophysics Data System (ADS)

    Frontera, T.; Olivera, C.; Jara, J. A.; Goula, X.; Ugalde, A.

    2009-04-01

    On August 2005 a permanent ocean bottom seismometer (OBS) and a differential pressure gauge (DPG) were installed inside the security perimeter of the Casablanca oil platform (40 km offshore Tarragona, NE Spain), within the framework of a project which has the aim of improving the knowledge of the seismicity and seismic risk in the region. This project is being carried out by the Institut Geològic de Catalunya (IGC) and the Observatori de l'Ebre, in collaboration with the Spanish oil company Repsol Investigaciones Petrolíferas. The sensors were submerged at about 400 m to the SW of the oil platform and were deposited at about 150 m in depth. Data are digitized on-site and are transmitted through a submarine cable to the platform, where they are recorded. In July 2007 via satellite signal transmission was implemented to have continuous and real time data, which allowed integrating the OBS into the Catalan Seismic Network. Since 1999 the objectives of the Catalan Seismic Network are, on the one hand, providing rapid information for Civil Defence Services and society in general and, on the other hand, to obtain systematically high quality data for the scientific community. This real time system is based on a VSAT seismic network and it has been implemented in Catalonia (Spain) by the IGC. The project of the network was planned to create robust, high performance field infrastructures through the installation of up to 21 stations equipped with three component broad band sensors and a high dynamic range and it has been developed in several steps. In 2009, 18 stations are operative: 14 broad band and 3 accelerometers on land and one broad band OBS. The stations are based on VSAT platforms that are transmitting continuously almost real time seismic data via satellite to the IGC hub. Once at seismic data reception centre data are continuously archived and processed with an automatic system. A study of the OBS signal in terms of noise has been made and compared to the

  14. VizieR Online Data Catalog: Properties of OB associations in IC 1613 (Garcia+, 2010)

    NASA Astrophysics Data System (ADS)

    Garcia, M.; Herrero, A.; Castro, N.; Corral, L.; Rosenberg, A.

    2014-06-01

    To understand the structure and evolution of massive stars, systematic surveys of the Local Group galaxies have been undertaken, to find these objects in environments of different chemical abundances. We focus on the metal-poor irregular galaxy IC 1613 to analyze the stellar and wind structure of its low-metallicity massive stars. We ultimately aim to study the metallicity-dependent driving mechanism of the winds of blue massive stars and use metal-poor massive stars of the Local Volume as a proxy for the stars in the early Universe. In a previous paper we produced a list of OB associations in IC 1613. Their properties are not only a powerful aid towards finding the most interesting candidate massive stars, but also reveal the structure and recent star formation history of the galaxy. We characterize these OB associations and study their connection with the galactic global properties. The reddening-free Q parameter is a powerful tool in the photometric analysis of young populations of massive stars, since it exhibits a smaller degree of degeneracy with OB spectral types than the B-V color. The color-magnitude diagram (Q vs. V) of the OB associations in IC 1613 is studied to determine their age and mass, and confirm the population of young massive stars. We identified more than 10 stars with M>=50M⊙. Spectral classification available for some of them confirm their massive nature, yet we find the common discrepancy with the spectroscopically derived masses. There is a general increasing trend of the mass of the most massive member with the number of members of each association, but not with the stellar density. The average diameter of the associations of this catalog is 40pc, half the historically considered typical size of OB associations. Size increases with the association population. The distribution of the groups strongly correlates with that of neutral and ionized hydrogen. We find the largest dispersion of association ages in the bubble region of the galaxy

  15. Increased hepatic oxidative metabolism distinguishes the action of Peroxisome proliferator-activated receptor δ from Peroxisome proliferator-activated receptor γ in the ob/ob mouse

    PubMed Central

    2009-01-01

    Background The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors and members of the nuclear receptor superfamily. The PPAR family consists of three members: PPARα, PPARγ, and PPARδ. PPARδ controls the transcription of genes involved in multiple physiological pathways, including cellular differentiation, lipid metabolism and energy homeostasis. The receptor is expressed almost ubiquitously, with high expression in liver and skeletal muscle. Although the physiological ligands of PPARδ remain undefined, a number of high affinity synthetic ligands have been developed for the receptor as a therapeutic target for type 2 diabetes mellitus, dyslipidemia and the metabolic syndrome. Methods In this study, the metabolic role of PPARδ activation has been investigated in liver, skeletal muscle, blood serum and white adipose tissue from ob/ob mice using a high affinity synthetic ligand and contrasted with PPARγ activation. To maximize the analytical coverage of the metabolome, 1H-nuclear magnetic resonance (1H-NMR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and ultra performance liquid chromatography-mass spectrometry (UPLC-MS) were used to examine metabolites from tissue extracts. Results Analysis by multivariate statistics demonstrated that PPARδ activation profoundly affected glycolysis, gluconeogenesis, the TCA cycle and linoleic acid and α-linolenic acid essential fatty acid pathways. Conclusions Although activation of both PPARδ and PPARγ lead to increased insulin sensitivity and glucose tolerance, PPARδ activation was functionally distinct from PPARγ activation, and was characterized by increased hepatic and peripheral fatty acid oxidative metabolism, demonstrating the distinctive catabolic role of this receptor compared with PPARγ. PMID:19968882

  16. Winds of low-metallicity OB-type stars: HST-COS spectroscopy in IC 1613

    SciTech Connect

    Garcia, Miriam; Najarro, Francisco; Herrero, Artemio; Urbaneja, Miguel Alejandro

    2014-06-10

    We present the first quantitative ultraviolet spectroscopic analysis of resolved OB stars in IC 1613. Because of its alleged very low metallicity (≲1/10 Z {sub ☉}, from H II regions), studies in this Local Group dwarf galaxy could become a significant step forward from the Small Magellanic Cloud (SMC) toward the extremely metal-poor massive stars of the early universe. We present HST-COS data covering the ∼1150-1800 Å wavelength range with resolution R ∼ 2500. We find that the targets do exhibit wind features, and these are similar in strength to SMC stars. Wind terminal velocities were derived from the observed P Cygni profiles with the Sobolev plus Exact Integration method. The v {sub ∞}-Z relationship has been revisited. The terminal velocity of IC 1613 O stars is clearly lower than Milky Way counterparts, but there is no clear difference between IC 1613 and SMC or LMC analog stars. We find no clear segregation with host galaxy in the terminal velocities of B-supergiants, nor in the v {sub ∞}/v {sub esc} ratio of the whole OB star sample in any of the studied galaxies. Finally, we present the first evidence that the Fe-abundance of IC 1613 OB stars is similar to the SMC, which is in agreement with previous results on red supergiants. With the confirmed ∼1/10 solar oxygen abundances of B-supergiants, our results indicate that IC 1613's α/Fe ratio is sub-solar.

  17. A Spitzer view of the giant molecular cloud Mon OB1 East/NGC 2264

    SciTech Connect

    Rapson, V. A.; Pipher, J. L.; Gutermuth, R. A.; Megeath, S. T.; Allen, T. S.; Myers, P. C.; Allen, L. E.

    2014-10-20

    We present Spitzer 3.6, 4.5, 5.8, 8.0, and 24 μm images of the Mon OB1 East giant molecular cloud, which contains the young star forming region NGC 2264, as well as more extended star formation. With Spitzer data and Two Micron All Sky Survey photometry, we identify and classify young stellar objects (YSOs) with dusty circumstellar disks and/or envelopes in Mon OB1 East by their infrared-excess emission and study their distribution with respect to cloud material. We find a correlation between the local surface density of YSOs and column density of molecular gas as traced by dust extinction that is roughly described as a power law in these quantities. NGC 2264 follows a power-law index of ∼2.7, exhibiting a large YSO surface density for a given gas column density. Outside of NGC 2264 where the surface density of YSOs is lower, the power law is shallower and the region exhibits a larger gas column density for a YSO surface density, suggesting the star formation is more recent. In order to measure the fraction of cloud members with circumstellar disks/envelopes, we estimate the number of diskless pre-main-sequence stars by statistical removal of background star detections. We find that the disk fraction of the NGC 2264 region is 45%, while the surrounding, more distributed regions show a disk fraction of 19%. This may be explained by the presence of an older, more dispersed population of stars. In total, the Spitzer observations provide evidence for heterogenous, non-coeval star formation throughout the Mon OB1 cloud.

  18. Nature vs. Nurture: The influence of OB star environments on proto-planetary disk evolution.

    NASA Astrophysics Data System (ADS)

    Bouwman, Jeroen; Feigelson, Eric; Getman, Kostantin; Henning, Thomas; Lawson, Warrick; Linz, Hendrik; Luhman, Kevin; Roccatagliata, Veronica; Sicilia Aguilar, Aurora; Townsley, Leisa; Wang, Junfeng

    2006-05-01

    A natural approach for understanding the origin and diversity of planetary systems is to study the birth sites of planetary systems under varying environmental conditions. Dust grains in protoplanetary disks, the building blocks of planets, are structurally and chemically altered, and grow through coagulation into planetesimals. The disk geometry may change from a flaring to a more flattened structure, gaps may develop under the gravitational influence of protoplanets, and eventually the disk will dissipate, terminating the planet formation process. While the infrared properties of disks in quiet cloud environments have been extensively studied, investigations under the conditions of strong UV radiation and stellar winds in the proximity of OB stars have been limited. We propose a combined IRAC/IRS study of a large, well-defined and unbiased X-ray selected sample of pre-main-sequence stars in three OB associations: Pismis 24 in NGC 6357, NGC 2244 in the Rosette Nebula, and IC 1795 in the W3 complex. The samples are based on recent Chandra X-ray Observatory studies which reliably identify hundreds of cluster members and were carefully chosen to avoid high infrared nebular background. A new Chandra exposure of IC 1795 is requested, and an optical followup to characterise the host stars is planned. Modelling the Spitzer findings will provide the composition and size of dust present as well as the geometry, mass, and gaps in the global structure of the disk. As hundreds of cluster members will be covered with IRAC and dozens with IRS, good statistics on the disk evolution and dispersal as a function of location with respect to OB stars will be obtained. Comparison of disk properties within our sample and with existing Spitzer studies of quiescent star-forming regions should significantly advance the aim of characterising the influence of the environment on the evolution of protoplanetary disks. This effort relies on a powerful synergy between the Chandra and Spitzer

  19. OB Associations at the Upper End of the Milky Way Luminosity Function

    NASA Astrophysics Data System (ADS)

    Rahman, Mubdi; Matzner, Christopher D.; Moon, Dae-Sik

    2013-04-01

    The Milky Way's most luminous, young, and massive (M gsim 104 M ⊙) star clusters and OB associations have largely evaded detection despite knowledge of their surrounding H II regions. We search for these clusters and associations within the 40 star-forming complexes from Rahman & Murray in the 13 most luminous Wilkinson Microwave Anisotropy Probe (WMAP) free-free emission sources of the Galaxy. Selecting for objects with the dust-reddened colors of OB stars, we identify new candidate associations using the Two Micron All Sky Survey point-source catalog. In 40 star-forming complexes searched, 22 contain cluster/association candidates with sizes and masses in the range of 3'-26' and 102.3-105 M ⊙. Of the 22 candidates, at least seven have estimated masses >~ 104 M ⊙, doubling the number of such massive clusters known in the Galaxy. Only one of the searched WMAP sources remains without a candidate. Applying our method to a statistically similar set of test locations, we estimate that 3.0 ± 0.6 of our 22 candidate associations are unrelated to the star-forming complexes. In addition, the apparent extinctions of our candidate associations correlate well with the predictions from a Galactic model. These facts, along with the clear detection of a known OB association and the previous spectral verification of one cluster found by this method, validate our method. In eight of the most luminous WMAP sources, the candidate associations can account for the observed free-free flux. With our new compilation, the Galactic census of young, massive stellar associations may now be about half complete.

  20. MUG-OBS - Multiparameter Geophysical Ocean Bottom System : a new instrumental approach to monitor earthquakes.

    NASA Astrophysics Data System (ADS)

    hello, yann; Charvis, Philippe; Yegikyan, Manuk; verfaillie, Romain; Rivet, Diane

    2016-04-01

    Real time monitoring of seismic activity is a major issue for early warning of earthquakes and tsunamis. It can be done using regional scale wired nodes, such as Neptune in Canada and in the U.S, or DONET in Japan. Another approach to monitor seismic activity at sea is to deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four time 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight time 6-months deployments) and the Osisec program in Ecuador (four time 6-months deployments). These autonomous OBSs are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data. Among the most recent alternative we developed a 3/4 years autonomy ocean bottom system with 9 channels (?) allowing the acquisition of different seismic or environmental parameters. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a crisis) using one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years, it is a perfect tool to monitor large seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. For remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two-way Iridium satellite communication. After a period of 3 years the main station is self-recovered by

  1. OB ASSOCIATIONS AT THE UPPER END OF THE MILKY WAY LUMINOSITY FUNCTION

    SciTech Connect

    Rahman, Mubdi; Matzner, Christopher D.; Moon, Dae-Sik

    2013-04-01

    The Milky Way's most luminous, young, and massive (M {approx}> 10{sup 4} M{sub Sun }) star clusters and OB associations have largely evaded detection despite knowledge of their surrounding H II regions. We search for these clusters and associations within the 40 star-forming complexes from Rahman and Murray in the 13 most luminous Wilkinson Microwave Anisotropy Probe (WMAP) free-free emission sources of the Galaxy. Selecting for objects with the dust-reddened colors of OB stars, we identify new candidate associations using the Two Micron All Sky Survey point-source catalog. In 40 star-forming complexes searched, 22 contain cluster/association candidates with sizes and masses in the range of 3'-26' and 10{sup 2.3}-10{sup 5} M{sub Sun }. Of the 22 candidates, at least seven have estimated masses {approx}> 10{sup 4} M{sub Sun }, doubling the number of such massive clusters known in the Galaxy. Only one of the searched WMAP sources remains without a candidate. Applying our method to a statistically similar set of test locations, we estimate that 3.0 {+-} 0.6 of our 22 candidate associations are unrelated to the star-forming complexes. In addition, the apparent extinctions of our candidate associations correlate well with the predictions from a Galactic model. These facts, along with the clear detection of a known OB association and the previous spectral verification of one cluster found by this method, validate our method. In eight of the most luminous WMAP sources, the candidate associations can account for the observed free-free flux. With our new compilation, the Galactic census of young, massive stellar associations may now be about half complete.

  2. SPITZER IMAGING OF THE NEARBY RICH YOUNG CLUSTER, Cep OB3b

    SciTech Connect

    Allen, Thomas S.; Kryukova, Erin; Thomas Megeath, S.; Gutermuth, Robert A.; Pipher, Judith L.; Naylor, Tim; Jeffries, R. D.; Wolk, Scott J.; Spitzbart, Brad; Muzerolle, James

    2012-05-10

    We map the full extent of a rich massive young cluster in the Cep OB3b association with the Infrared Array Camera and Multi-band Imaging Photometer System instruments aboard the Spitzer Space Telescope and the ACIS instrument aboard the Chandra X-Ray Observatory. At 700 pc, it is revealed to be the second nearest large (>1000 member), young (<5 Myr) cluster known. In contrast to the nearest large cluster, the Orion Nebula Cluster, Cep OB3b is only lightly obscured and is mostly located in a large cavity carved out of the surrounding molecular cloud. Our infrared and X-ray data sets, as well as visible photometry from the literature, are used to take a census of the young stars in Cep OB3b. We find that the young stars within the cluster are concentrated in two sub-clusters; an eastern sub-cluster, near the Cep B molecular clump, and a western sub-cluster, near the Cep F molecular clump. Using our census of young stars, we examine the fraction of young stars with infrared excesses indicative of circumstellar disks. We create a map of the disk fraction throughout the cluster and find that it is spatially variable. Due to these spatial variations, the two sub-clusters exhibit substantially different average disk fractions from each other: 32% {+-} 4% and 50% {+-} 6%. We discuss whether the discrepant disk fractions are due to the photodestruction of disks by the high mass members of the cluster or whether they result from differences in the ages of the sub-clusters. We conclude that the discrepant disk fractions are most likely due to differences in the ages.

  3. ObsPy: A Python toolbox for seismology - Current state, applications, and ecosystem around it

    NASA Astrophysics Data System (ADS)

    Krischer, L.; Megies, T.; Sales de Andrade, E.; Barsch, R.; Beyreuther, M.

    2015-12-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project offering a bridge for seismology into the scientific Python ecosystem. It provides read and write support for essentially all commonly used waveform, station, and event metadata formats with a unified interface, a comprehensive signal processing toolbox tuned to the needs of seismologists, integrated access to all large data centers, web services and databases, and convenient wrappers to third party codes like libmseed and evalresp. Python, in contrast to many other languages and tools, is simple enough to enable an exploratory and interactive coding style desired by many scientists. At the same time it is a full-fledged programming language usable by software engineers to build complex and large programs. This combination makes it very suitable for use in seismology where research code often has to be translated to stable and production ready environments. It furthermore offers many freely available high quality scientific modules covering most needs in developing scientific software.ObsPy has been in constant development for more than 5 years and nowadays enjoys a large rate of adoption in the community with thousands of users. Successful applications include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. Additionally it sparked the development of several more specialized packages slowly building a modern seismological ecosystem around it.This contribution will give a short introduction and overview of ObsPy and highlight a number of us cases and software built around it. We will furthermore discuss the issue of sustainability of scientific software.

  4. An analysis of HST UV spectra of Cyg OB2 stars

    NASA Astrophysics Data System (ADS)

    Herrero, A.; Puls, J.; Corral, L. J.; Kudritzki, R. P.; Villamariz, M. R.

    2001-02-01

    As a first step of a vigorous program to investigate the Wind Momentum - Luminosity Relationship (WLR) of Galactic O-stars by analyzing stars belonging to the same cluster we present UV HST observations of six supergiants and one giant in the Cyg OB2 association. Terminal and turbulent wind velocities, velocity laws and metal ion column densities are derived and mean ionization fractions are estimated. Turbulent velocities are mostly in the range 10-14% of v_infty . The terminal velocities agree well with the average v_infty vs. spectral class relationship compiled by Kudritzki & Puls (\\cite{kp00}). We compare the observed v_infty vs. escape velocity (depending on the diagnostics of the stellar mass) correlation with the predictions of the radiatively driven wind theory and find better agreement with the spectroscopic masses rather than with the evolutionary ones. The beta velocity field exponents are in the range 0.7-0.8, without any trend towards larger values. We show that for a single luminosity class there is a tight relationship between T_eff and (the mean density at the point in the stellar wind, where half the wind terminal velocity is reached). In consequence, the ionization fractions show the same trend with both, T_eff and : we find that N V increases with T_eff, Si Iv decreases and C Iv does not clearly correlate. As a byproduct, we also derive interestellar H I column densities towards Cyg OB2, which turn out to be quite large. For one object (Cyg OB2#2) we find inconsistencies making the association membership questionable. Partly based on INES data from the IUE satellite.

  5. ObsPy: A Python toolbox for seismology - Current state, applications, and ecosystem around it

    NASA Astrophysics Data System (ADS)

    Lecocq, Thomas; Megies, Tobias; Krischer, Lion; Sales de Andrade, Elliott; Barsch, Robert; Beyreuther, Moritz

    2016-04-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project offering a bridge for seismology into the scientific Python ecosystem. It provides * read and write support for essentially all commonly used waveform, station, and event metadata formats with a unified interface, * a comprehensive signal processing toolbox tuned to the needs of seismologists, * integrated access to all large data centers, web services and databases, and * convenient wrappers to third party codes like libmseed and evalresp. Python, in contrast to many other languages and tools, is simple enough to enable an exploratory and interactive coding style desired by many scientists. At the same time it is a full-fledged programming language usable by software engineers to build complex and large programs. This combination makes it very suitable for use in seismology where research code often has to be translated to stable and production ready environments. It furthermore offers many freely available high quality scientific modules covering most needs in developing scientific software. ObsPy has been in constant development for more than 5 years and nowadays enjoys a large rate of adoption in the community with thousands of users. Successful applications include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. Additionally it sparked the development of several more specialized packages slowly building a modern seismological ecosystem around it. This contribution will give a short introduction and overview of ObsPy and highlight a number of use cases and software built around it. We will furthermore discuss the issue of sustainability of scientific software.

  6. The INGV's new OBS/H: Analysis of the signals recorded at the Marsili submarine volcano

    NASA Astrophysics Data System (ADS)

    D'Alessandro, Antonino; D'Anna, Giuseppe; Luzio, Dario; Mangano, Giorgio

    2009-05-01

    The ocean bottom seismometer with hydrophone deployed on the flat top of the Marsili submarine volcano (790 m deep) by the Gibilmanna OBS Lab (CNT-INGV) from 12th to 21st July, 2006, recorded more than 1000 transient seismic signals. Nineteen of these signals were associated with tectonic earthquakes: 1 teleseismic, 8 regional (located by INGV) and 10 small local seismic events (non located earthquakes). The regional events were used to determine sensor orientation. By comparing the signals recorded with typical volcanic seismic activity, we were able to group all the other signals into three categories: 817 volcano-tectonic type B (VT-B) events, 159 occurrences of high frequency tremor (HFT) and 32 short duration events (SDE). Small-magnitude VT-B swarms, having a frequency band of 2-6 Hz and a mean length of about 30 s, were almost all recorded during the first 7 days. During the last 2 days, the OBS/H mainly recorded HFT events with frequencies of over 40 Hz and of a few minutes in length. Signals that have similar features in frequency and time domain are generally associated with hydrothermal activity. During the last two days a signal was recorded that had a frequency content similar to that of VT-B events was recorded. It will be referred to as continuous volcanic tremor (CVT). The SDE signals, characterized by a quasi-monochromatic waveform and having an exponential decaying envelope, may have been generated by oscillations of resonant bodies excited by magmatic or hydrothermal activity. By applying polarization and parametric spectral analyses, we inferred that the VT-B were probably multi P-phase events having shallow sources that were situated in narrow azimuthal windows in relation to the positions of the OBS/H. The parametric spectral analysis of the SDE signals allowed us to determine their dominant complex frequencies with high accuracy; these frequencies are distributed in two distinct clusters on the complex plane.

  7. Interstellar Environments and Dust Properties toward Cygnus OB2 No. 12: A Reassessment

    NASA Astrophysics Data System (ADS)

    Whittet, D. C. B.

    2015-10-01

    The B-type hypergiant Cygnus OB2 no. 12 is a popular target for studies of interstellar phenomena at visible-infrared wavelengths because of its exceptional brightness for a star dimmed by some 10 mag of visual extinction. A lack of detectable ice absorption has led investigators to regard the line of sight as a standard for studies of the “diffuse” interstellar medium (ISM), an assumption challenged both by observations of molecular gas toward the star and by uncertainties concerning the degree to which such a luminous object may affect its local environment. This paper presents a reassessment of the nature of the material responsible for extinction toward Cyg OB2 no. 12. The excess relative to other cluster members appears to occur in translucent clumps within an extensive network of clouds in the region. Attenuation of the ambient radiation field is sufficient in the cores of the clumps to support the presence of gas-phase molecules, but not to sustain detectable ice formation. In general, the optical properties of dust in the clumps are closely similar to those observed in typical diffuse interstellar material, with the notable exception of an unusually low value for the wavelength of maximum polarization. The implied enhancement of polarization by small grains is attributed to increased alignment efficiency in an enhanced magnetic field. This caveat apart, the results of the current paper provide reassurance that Cyg OB2 no. 12 is, indeed, an appropriate choice for studies that target diffuse and translucent phases of the ISM.

  8. INTERSTELLAR ENVIRONMENTS AND DUST PROPERTIES TOWARD CYGNUS OB2 NO. 12: A REASSESSMENT

    SciTech Connect

    Whittet, D. C. B.

    2015-10-01

    The B-type hypergiant Cygnus OB2 no. 12 is a popular target for studies of interstellar phenomena at visible-infrared wavelengths because of its exceptional brightness for a star dimmed by some 10 mag of visual extinction. A lack of detectable ice absorption has led investigators to regard the line of sight as a standard for studies of the “diffuse” interstellar medium (ISM), an assumption challenged both by observations of molecular gas toward the star and by uncertainties concerning the degree to which such a luminous object may affect its local environment. This paper presents a reassessment of the nature of the material responsible for extinction toward Cyg OB2 no. 12. The excess relative to other cluster members appears to occur in translucent clumps within an extensive network of clouds in the region. Attenuation of the ambient radiation field is sufficient in the cores of the clumps to support the presence of gas-phase molecules, but not to sustain detectable ice formation. In general, the optical properties of dust in the clumps are closely similar to those observed in typical diffuse interstellar material, with the notable exception of an unusually low value for the wavelength of maximum polarization. The implied enhancement of polarization by small grains is attributed to increased alignment efficiency in an enhanced magnetic field. This caveat apart, the results of the current paper provide reassurance that Cyg OB2 no. 12 is, indeed, an appropriate choice for studies that target diffuse and translucent phases of the ISM.

  9. A Multi-Fiber Spectroscopic Search for Low-mass Young Stars in Orion OB1

    NASA Astrophysics Data System (ADS)

    Loerincs, Jacqueline; Briceno, Cesar; Calvet, Nuria; Mateo, Mario L.; Hernandez, Jesus

    2017-01-01

    We present here results of a low resolution spectroscopic followup of candidate low-mass pre-main sequence stars in the Orion OB1 association. Our targets were selected from the CIDA Variability Survey of Orion (CVSO), and we used the Michigan/Magellan Fiber Spectrograph (M2FS) on the Magellan Clay 6.5m telescope to obtain spectra of 500 candidate T Tauri stars distributed in seven 0.5 deg diameter fields, adding to a total area of ~5.5 deg2. We identify young stars by looking at the distinctive Hα 6563 Å emission and Lithium Li I 6707 Å absorption features characteristic of young low mass pre-main sequence stars. Furthermore, by measuring the strength of their Hα emission lines, confirmed T Tauri stars can be classified as either Classical T Tauris (CTTS) or Weak-line T Tauris (WTTS), which give indication of whether the star is actively accreting material from a gas and dust disk surrounding the star, which may be the precursor of a planetary system. We confirm a total of 90 T Tauri stars, of which 50% are newly identified young members of Orion; out of the 49 new detections,15 are accreting CTTS, and of these all but one are found in the OB1b sub-region. This result is in line with our previous findings that this region is much younger than the more extended Orion OB1a sub-association. The M2FS results add to our growing census of young stars in Orion, that is allowing us to characterize in a systematic and consistent way the distribution of stellar ages across the entire complex, in order to building a complete picture of star formation in this, one of nearest most active sites of star birth.

  10. The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs

    PubMed Central

    Kolar, Carol; Yan, Ying; Borgstahl, Gloria E.O.; Ouellette, Michel M.

    2015-01-01

    The POT1 protein plays a critical role in telomere protection and telomerase regulation. POT1 binds single-stranded 5’-TTAGGGTTAG-3’ and forms a dimer with the TPP1 protein. The dimer is recruited to telomeres, either directly or as part of the Shelterin complex. Human POT1 contains two Oligonucleotide/Oligosaccharide Binding (OB) fold domains, OB1 and OB2, which make physical contact with the DNA. OB1 recognizes 5’-TTAGGG whereas OB2 binds to the downstream TTAG-3’. Studies of POT1 proteins from other species have shown that some of these proteins are able to recognize a broader variety of DNA ligands than expected. To explore this possibility in humans, we have used SELEX to reexamine the sequence-specificity of the protein. Using human POT1 as a selection matrix, high-affinity DNA ligands were selected from a pool of randomized single-stranded oligonucleotides. After six successive rounds of selection, two classes of high-affinity targets were obtained. The first class was composed of oligonucleotides containing a cognate POT1 binding sites (5’-TTAGGGTTAG-3’). The second and more abundant class was made of molecules that carried a novel non-telomeric consensus: 5’-TNCANNAGKKKTTAGG-3’ (where K=G/T and N=any base). Binding studies showed that these non-telomeric sites were made of an OB1-binding motif (TTAGG) and a non-telomeric motif (NT motif), with the two motifs recognized by distinct regions of the OB1 domain. POT1 interacted with these non-telomeric binding sites with high affinity and specificity, even when bound to its dimerization partner TPP1. This intrinsic ability of POT1 to recognize NT motifs raises the possibility that the protein may fulfill additional functions at certain non-telomeric locations of the genome, in perhaps gene transcription, replication, or repair. PMID:25934589

  11. Ocean Basalt Simulator version 1 (OBS1): Trace element mass balance in adiabatic melting of a pyroxenite-bearing peridotite

    NASA Astrophysics Data System (ADS)

    Kimura, J. I.; Kawabata, H.

    2014-12-01

    We present a new numerical mass balance model for estimating the source conditions of a primary ocean basalt from adiabatic melting of a pyroxenite-bearing peridotite. The Ocean Basalt Simulator version 1 (OBS1) uses a thermodynamic model of adiabatic melting of a pyroxenite-bearing peridotite with experimentally parameterized liquidus-solidus and melting intervals and thermodynamically/experimentally parameterized source mineralogy of pyroxenite and peridotite. OBS1 uses a sequence of adiabatic melting with pyroxenite melting, melt metasomatism in the host peridotite, and melting of the metasomatized peridotite. OBS1 explores (1) the fractions of peridotite and pyroxenite, (2) mantle potential temperature Tp, (3) depth of termination of melting Pmt, (4) degree of melting F, and (5) residual mode Xa of the sources. The model also examines the mass balance of 26 incompatible trace elements in the sources and in the generated basalt. OBS1 can also calculate Sr-Nd-Hf-Pb isotope compositions in the sources and in the melt. OBS1 is coded in an Excel spreadsheet and runs with VBA macros. Using OBS1, we examine the source conditions of the mid oceanic ridge basalts (MORBs), plume-influenced ocean ridge basalts in Galápagos, Loihi-Loa-Koolau basalts in the Hawaiian hotspot, high-mu (HIMU) basalt from the St. Helena hotspot, and Shatsky Rise and Jurassic Mikabu oceanic plateau basalts and picrites. OBS1 is a unique model that provides an opportunity to examine the source mantle conditions using incompatible trace elements and relevant isotopes, which are key factors in global mantle recycling.

  12. Detection of H3+ in the diffuse interstellar medium toward Cygnus OB2 No. 12.

    PubMed

    McCall, B J; Geballe, T R; Hinkle, K H; Oka, T

    1998-03-20

    The molecular ion H3+ is considered the cornerstone of interstellar chemistry because it initiates the reactions responsible for the production of many larger molecules. Recently discovered in dense molecular clouds, H3+ has now been observed in the diffuse interstellar medium toward Cygnus OB2 No. 12. Analysis of H3+ chemistry suggests that the high H3+ column density (3.8 x 10(14) per square centimeter) is due not to a high H3+ concentration but to a long absorption path. This and other work demonstrate the ubiquity of H3+ and its potential as a probe of the physical and chemical conditions in the interstellar medium.

  13. Nature vs. Nurture: The influence of OB star environments on proto-planetary disk evolution

    NASA Astrophysics Data System (ADS)

    Bouwman, Jeroen

    2006-09-01

    We propose a combined IRAC/IRS study of a large, well-defined and unbiased X-ray selected sample of pre-main-sequence stars in three OB associations: Pismis 24 in NGC 6357, NGC 2244 in the Rosette Nebula, and IC 1795 in the W3 complex. The samples are based on recent Chandra X-ray Observatory studies which reliably identify hundreds of cluster members and were carefully chosen to avoid high infrared nebular background. A new Chandra exposure of IC 1795 is requested, and an optical followup to characterise the host stars is planned.

  14. Technical Memorandum: A Training Evaluation Framework for PSTC MilObs Course

    DTIC Science & Technology

    2009-09-01

    préparent à partir en mission en tant qu’observateurs militaires des Nations Unies (UNMO ou Ob mil) entreprennent un certain nombre d’activités...un certain nombre de processus psychologiques au cours de ce scénario, tels que les aspects de l’intensité morale (proximité physique de la victime...entreprennent un certain nombre d’activités d’entraînement destinées à améliorer et développer les compétences propres aux Opérations de paix des

  15. Synthetic filter photometry and evolutionary status of two Be stars in the association Per OB1

    NASA Technical Reports Server (NTRS)

    Torres, Ana V.

    1987-01-01

    Stromgren and H-beta colors have been determined from spectrophotometric observations for two Be stars without published photometry in the association Per OB1: HD 12856 (B0 pe) and HD 13890 (B1 III:pe). Stellar parameters and improved spectral types are then derived from the color indices using the calibrations of Jakobsen (1986), and independently from the BCD classification method. The intrinsic properties of HD 12856 and HD 13890 are compared with those of normal B stars and are used to estimate their evolutionary status.

  16. Luminosity functions and color-magnitude diagrams for three OB associations in the Large Magellanic Cloud

    NASA Technical Reports Server (NTRS)

    Degioia-Eastwood, K.; Meyers, R. P.; Jones, D. P.

    1993-01-01

    Using the point spread function photometry program DAOPHOT, we have used UBV CCD photometry to construct color-magnitude diagrams and luminosity functions for three OB associations in the Large Magellanic Cloud. The region LH 76 appears to be completely coeval; the region LH 13 shows some evidence for noncoevality which will need to be checked with spectra of the stars in question. The region LH 105, which lies on the southern edge of 30 Doradus, shows significant contamination by an underlying older population, possibly from previous star forming events. The luminosity functions, which serve as the first step toward determining the initial mass function in these regions, are calculated.

  17. Synthetic filter photometry and evolutionary status of two Be stars in the association Per OB1

    NASA Technical Reports Server (NTRS)

    Torres, Ana V.

    1987-01-01

    Stromgren and H-beta colors have been determined from spectrophotometric observations for two Be stars without published photometry in the association Per OB1: HD 12856 (B0 pe) and HD 13890 (B1 III:pe). Stellar parameters and improved spectral types are then derived from the color indices using the calibrations of Jakobsen (1986), and independently from the BCD classification method. The intrinsic properties of HD 12856 and HD 13890 are compared with those of normal B stars and are used to estimate their evolutionary status.

  18. BOREAS TF-8 NSA-OJP and SSA-OBS Ceilometer Data

    NASA Technical Reports Server (NTRS)

    Moore, Kathleen E.; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Fitzjarrald, David R.

    2000-01-01

    The BOREAS TF-8 team used ceilometers to collect data on the fraction of the sky covered with clouds and the cloud height. Included with these data is the surface-based lifting condensation level, derived from temperature and humidity values acquired at the flux tower at the NSA-OJP site. Ceilo-meter data were collected at the NSA-OJP site in 1994 and at the NSA-OJP and SSA-OBS sites in 1996. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884).

  19. Mechanisms of action of otilonium bromide (OB) in human cultured smooth muscle cells and rat colonic strips.

    PubMed

    Martínez-Cutillas, M; Gil, V; Gallego, D; Mañé, N; Martín, M T; Jiménez, M

    2013-12-01

    The pharmacological properties of otilonium bromide (OB) have been investigated using different experimental models, techniques, and conditions, and consequently, the results are not always easy to compare. The aim of the present work was to investigate the pharmacological properties of OB in human cultured colonic smooth muscle cells (HCSMCs), which is the main target of the drug 'in vivo'. Rat colonic strips were used to confirm the pharmacological properties. Human cultured colonic smooth muscle cells were studied using the calcium imaging technique. Microelectrodes and muscle bath experiments were performed in rat colonic strips. Otilonium bromide (OB) concentration dependently inhibited nifedipine-sensitive calcium transients induced by KCl (EC50  = 3.6 μM) and BayK8644 (EC50  = 4.0 μM). All the following experiments were performed in the presence of nifedipine. In HCSMC, carbachol-induced calcium transients were inhibited by OB (EC50  = 8.4 μM). Carbachol evoked 1-a smooth muscle depolarization (10 mV) that was antagonized by 100 μM OB; and 2-a contraction that was inhibited by OB (EC50  = 13.0 μM). 'Non-nitrergic (L-NNA 1 mM) non-purinergic (MRS2500 1 μM)' conditions were used to elicit endogenous excitatory responses. Electrical field stimulation caused 1-an atropine-sensitive excitatory junction potential that was inhibited by OB (EC50  = 8.9 μM) and 2-an atropine-sensitive contraction that was inhibited by OB (EC50  = 7.3 μM). In HCSMC, neurokinin A (NKA) and CaCl2 induced calcium transients that were inhibited by OB (NKA: EC50  = 11.7 μM; CaCl2 : EC50  = 17.5 μM). Otilonium bromide causes inhibition of L-/T-type calcium channels, muscarinic, and tachykininergic responses that acting together explain the pharmacological properties of the compound. © 2013 John Wiley & Sons Ltd.

  20. Stellar Parameters of A- and B-type Members of the Scorpius-Centaurus OB Association

    NASA Astrophysics Data System (ADS)

    Eckelkamp, Grant; Smith, Skylar; Pecaut, Mark; Mamajek, Eric E.

    2017-01-01

    Advances in high-contrast imaging and radio interferometry are allowing new avenues for circumstellar disk studies as well as direct imaging exoplanet searches. The most promising targets in these studies are intermediate mass B/A/F type members of the Scorpius-Centaurus OB association at a distance of ~100-200 pc and an age of 10-20 Myr old. However, many of the most interesting stellar members of Sco-Cen have poorly constrained stellar properties, including luminosities, distances and masses. In order to address this, we have obtained low resolution spectra from the SMARTS 1.5 m telescope and have obtained new spectral classifications for 106 stars. Using these new spectral classifications along with visual and near-infrared photometry from GAIA, Hipparcos, Tycho2, APASS and 2MASS, we obtained new reddening and extinction estimates. We then placed the stars on a Hertzsprung-Russell diagram and compared with stellar evolutionary tracks to obtain individual masses and ages. These improved stellar parameters will provide essential data for improving our understanding of high contrast imaging targets of B/A/F type members within the nearest OB association. We acknowledge support from NSF awards AST-1008908 and AST-1313029.

  1. BOREAS TGB-8 Starch Concentration Data Over the SSA-OBS and the SSA-OJP

    NASA Technical Reports Server (NTRS)

    Lerdau, Manuel; Hall, Forrest G. (Editor); Conrad, Sara K. (Editor)

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set includes measurements of starch concentrations in foliar samples at mature jack pine and black spruce sites. The data were collected at the OJP and OBS tower flux locations in the BOREAS SSA. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  2. THE ORIGIN OF OB CLUSTERS: FROM 10 pc TO 0.1 pc

    SciTech Connect

    Liu Hauyu Baobab; Wang Ke; Ho, Paul T. P.; Zhang Qizhou; Quintana-Lacaci, Guillermo; Li Zhiyun; Zhang Zhiyu E-mail: kwang@cfa.harvard.edu E-mail: quintana@iram.es E-mail: zl4h@virginia.edu

    2012-01-20

    We observe the 1.2 mm continuum emission around the OB cluster-forming region G10.6-0.4, using the MAMBO-2 bolometer array of the IRAM 30 m telescope and the Submillimeter Array (SMA). Comparison of the Spitzer 24 {mu}m and 8 {mu}m images with our 1.2 mm continuum maps reveal an ionization front of an H II region, the photon-dominated layer, and several 5 pc scale filaments that follow the outer edge of the photon-dominated layer. The filaments, which are resolved in the MAMBO-2 observations, show regularly spaced parsec-scale molecular clumps, embedded with a cluster of dense molecular cores as shown in the SMA 0.87 mm observations. Toward the center of the G10.6-0.4 region, the combined SMA+IRAM 30 m continuum image reveals several parsec-scale protrusions. They may continue down to within 0.1 pc of the geometric center of a dense 3 pc scale structure, where a 200 M{sub Sun} OB cluster resides. The observed filaments may facilitate mass accretion onto the central cluster-forming region in the presence of strong radiative and mechanical stellar feedback. Their filamentary geometry may also facilitate fragmentation. We did not detect any significant polarized emission at 0.87 mm in the inner 1 pc region with SMA.

  3. BOREAS TGB-8 Monoterpene Concentration Data over the SSA-OBS

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Conrad, Sara K. (Editor); Lerdau, Manuel

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set contains measurements of monoterpene concentrations in collected foliar gas emissions and foliar samples. The data were collected at the OJP and OBS tower flux sites in the SSA and were the locus for the monoterpene emission measurements. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  4. Trichloroethylene degradation using recombinant bacteria expressing the soluble methane monooxygenase from methylosinus trichosporium OB3b

    SciTech Connect

    Jahng, D.; Kim, C.; Wood, T.K.

    1995-12-01

    Soluble methane monooxygenase (sMMO) from M. trichosporium OB3b has the ability to degrade many halogenated aliphatic compounds that are found in contaminated soil and groundwater. For efficient trichloroethylene (TCE) degradation in a foreign host, efforts are being made to improve inconsistent and low sMMO activity of the recombinant strain constructed previously (Pseudomonas putida F1/pSMMO20). Additional smmo-containing recombinant strains have been constructed including various Pseudomonas, Agrobacterium, and Rhizobium strains. Recombinant facultative methylotrophs containing the smmo locus were also constructed through electroporation and tri-parental mating using a new plasmid pSMMO50. TCE degradation by these recombinant strains was examined. The effect of metal ions on in vitro sMMO activity was also discerned to optimize the expression medium. Among the metal ions examined, Cu(I), Cu(II), Ni(II), and Zn(II) inhibited sMMO purified from trichosporium OB3b, and the effect of the metal ions on each of the components of sMMO will also be discussed. In addition, the post-segregational killing locus (hok/sok) from E. coli plasmid R1 was inserted downstream of the smmo locus to stabilize the recombinant plasmid in these host cells, and chemostat cultures were used to optimize expression of active sMMO by varying the growth rate.

  5. Accretion and outflow in the proplyd-like objects near Cygnus OB2

    SciTech Connect

    Guarcello, M. G.; Drake, J. J.; Wright, N. J.; García-Alvarez, D.; Kraemer, K. E.

    2014-09-20

    Cygnus OB2 is the most massive association within 2 kpc from the Sun, hosting hundreds of massive stars, thousands of young low mass members, and some sights of active star formation in the surrounding cloud. Recently, 10 photoevaporating proplyd-like objects with tadpole-shaped morphology were discovered in the outskirts of the OB association, approximately 6-14 pc away from its center. The classification of these objects is ambiguous, being either evaporating residuals of the parental cloud that are hosting a protostar inside or disk-bearing stars with an evaporating disk, such as the evaporating proplyds observed in the Trapezium Cluster in Orion. In this paper, we present a study based on low-resolution optical spectroscopic observations made with the Optical System for Imaging and low Resolution Integrated Spectroscopy, mounted on the 10.4 m Gran Telescopio CANARIAS, of two of these protostars. The spectrum of one of the objects shows evidence of accretion but not of outflows. In the latter object, the spectra show several emission lines indicating the presence of an actively accreting disk with outflow. We present estimates of the mass loss rate and the accretion rate from the disk, showing that the former exceeds the latter as observed in other known objects with evaporating disks. We also show evidence of a strong variability in the integrated flux observed in these objects as well as in the accretion and outflow diagnostics.

  6. Phenotypic characterization of copper-resistant mutants of Methylosinus trichosporium OB3b.

    PubMed Central

    Fitch, M W; Graham, D W; Arnold, R G; Agarwal, S K; Phelps, P; Speitel, G E; Georgiou, G

    1993-01-01

    Cultures of Methylosinus trichosporium OB3b grown in the presence of very low concentrations of copper synthesize a soluble methane monooxygenase (sMMO) that efficiently catalyzes the oxidation of trichloroethylene and other organic pollutants. Recently, we isolated five M. trichosporium OB3b mutants that express sMMO activity when grown in the presence of elevated copper concentrations (P.A. Phelps, S. K. Agarwal, G. E. Speitel, Jr., and G. Georgiou, Appl. Environ. Microbiol. 58:3701-3708, 1992). Here we show that, in contrast to the results for the wild-type cells, the addition of copper to mutant cultures grown on methane and nitrate as the nitrogen source has no noticeable effect on the growth rate and sMMO expression. In vitro experiments indicated that the copper-resistant phenotype does not arise from an increased stability of sMMO to copper deactivation. Furthermore, the mutant cultures exhibit altered speciation of copper in the extracellular fluid and have substantially decreased levels of cell-associated copper. On the basis of these results, we propose that the mutant phenotype arises from defects in copper uptake and metabolism rather than from changes in sMMO expression or enzyme stability. Images PMID:8215352

  7. ObsPy: a bridge for seismology into the scientific Python ecosystem

    NASA Astrophysics Data System (ADS)

    Krischer, Lion; Megies, Tobias; Barsch, Robert; Beyreuther, Moritz; Lecocq, Thomas; Caudron, Corentin; Wassermann, Joachim

    2015-01-01

    The Python libraries NumPy and SciPy are extremely powerful tools for numerical processing and analysis well suited to a large variety of applications. We developed ObsPy (http://obspy.org), a Python library for seismology intended to facilitate the development of seismological software packages and workflows, to utilize these abilities and provide a bridge for seismology into the larger scientific Python ecosystem. Scientists in many domains who wish to convert their existing tools and applications to take advantage of a platform like the one Python provides are confronted with several hurdles such as special file formats, unknown terminology, and no suitable replacement for a non-trivial piece of software. We present an approach to implement a domain-specific time series library on top of the scientific NumPy stack. In so doing, we show a realization of an abstract internal representation of time series data permitting I/O support for a diverse collection of file formats. Then we detail the integration and repurposing of well established legacy codes, enabling them to be used in modern workflows composed in Python. Finally we present a case study on how to integrate research code into ObsPy, opening it to the broader community. While the implementations presented in this work are specific to seismology, many of the described concepts and abstractions are directly applicable to other sciences, especially to those with an emphasis on time series analysis.

  8. Analysis of the kinematic structure of the Cygnus OB1 association

    NASA Astrophysics Data System (ADS)

    Costado, M. T.; Alfaro, E. J.; González, M.; Sampedro, L.

    2017-03-01

    The main objective of this study is the characterization of the velocity field in the Cygnus OB1 association using the radial velocity data currently available in the literature. This association is part of a larger star-forming complex located in the direction of the Cygnus region, but whose main subsystems may be distributed at different distances from the sun. We have collected radial velocity data for more than 300 stars in the area of 5 × 5 deg2 centred on the Cygnus OB1 association. We present the results of a kinematic clustering analysis in the subspace of the phase space formed by angular coordinates and radial velocity using two independent methodologies. We have found evidence of structure in the phase space with the detection of two main groups, corresponding to different radial velocity and distance values, belonging to the association, and associated with two main shells defined by the Hα emission. A third grouping well separated from the other two in velocity appears to occupy the whole region associated with what has been called 'common shell'.

  9. Analysis of interstellar extinction towards the hypergiant Cyg OB2 No. 12

    NASA Astrophysics Data System (ADS)

    Maryeva, O. V.; Chentsov, E. L.; Goranskij, V. P.; Karpov, S. V.

    The Cyg OB2 stellar association hosts an entire zoo of unique objects, and among them -- an enigmatic star Cyg OB2 No. 12 (Schulte 12, MT 304). MT 304 is enigmatic not only due to its highest luminosity (according to various estimates, it is one of the brightest stars in the Galaxy), but also because its reddening is anomalously large, greater than the mean reddening in the association. To explain the nature of anomalous reddening (AV≃ 10 mag) of MT 304, we carried out spectrophotometric observations of 25 stars located in its vicinity. We mapped interstellar extinction within the 2.5 arcmin radius and found it to increase towards MT 304. According to our results, the two most reddened stars in the association after MT 304 are J203240.35+411420.1 and J203239.90+411436.2, both located about 15 arcsec from it. Interstellar extinction AV towards these stars is about 9 mag. Our results favor the hypothesis of the circumstellar nature of reddening excess. In the second part of the paper we present the results of our modeling of MT 282 (B1 IV) and MT 343 (B1 V), which belong to the older population of the association and have ages greater than 10 Myr.

  10. A model for the infrared emission from an OB star cluster environment

    NASA Technical Reports Server (NTRS)

    Leisawitz, D.

    1991-01-01

    A model for the infrared emission from the neighborhood of an OB star cluster is described. The distribution of gas and dust around the stars, properties of the dust, and the cluster and interstellar radiation fields are variable. The model can be applied to regions around clusters embedded to various degrees in their parental molecular clouds (i.e., compact H II regions, blister-type H II regions, and the tenuous H II regions ionized by naked O stars). The model is used to simulate IRAS observations of a typical blister H II region. Infrared surface brightness and spectral energy distributions are predicted and the impact of limited spatial resolution is illustrated. The model results are shown to be consistent with observations of the exemplary outer Galaxy OB cluster NGC 7380. It is planned to use the model as a diagnostic tool to probe the physical conditions and dust properties in star-formation regions and, ultimately, in an interpretation of the spectral energy distributions of spiral galaxies.

  11. Molecular Gas toward the Gemini OB1 Molecular Cloud Complex. I. Observation Data

    NASA Astrophysics Data System (ADS)

    Wang, Chen; Yang, Ji; Xu, Ye; Li, Facheng; Su, Yang; Zhang, Shaobo

    2017-05-01

    We present a large-scale mapping toward the GEM OB1 association in the galactic anti-center direction. The 9° × 6.°5 area was mapped in 12CO, 13CO, and C18O with ˜50″ angular resolution at 30″ sampling. The region was divided into four main components based on spatial distribution and velocity: the Gemini OB1 Giant Molecular Cloud (GGMC) Complex, the Lynds Dark Clouds and the West Front Clouds, the Swallow and Horn, and the Remote Clouds. The GGMC Complex is located in the Perseus arm, while the Lynds Dark Clouds and the West Front Clouds are located in the Local arm. Swallow and Horn are revealed for the first time in this paper. The two clouds have a similar velocity interval ([11, 21] km s-1) and have similar sizes (0.6 and 0.8 deg2). We analyzed the structure of these clouds in detail and calculated their parameters (mass, temperature, etc.). Two elongated structures were discovered in a longitude-velocity map in the velocity interval [11, 30] km s-1. We also found an interesting filament that shows a 0.8 km s-1 pc-1 gradient perpendicular to the direction of the long axis.

  12. La binaria LSS 3074 y su entorno: ?`una nueva asociación OB?

    NASA Astrophysics Data System (ADS)

    Niemela, V.; Morrell, N.; Corti, M.

    En este trabajo presentamos un nuevo análisis orbital de LSS~3074, junto con tipos espectrales y velocidades radiales de estrellas que podrían constituir con ella una nueva asociación OB. La estrella O4f LSS3074 fue descubierta como binaria espectroscópica de corto período y líneas dobles por Morrell & Niemela (1990, ASP Conf. Ser. 7, 57). Posteriormente, Haefner et~al.(1994, IBVS 3969) encontraron variaciones fotométricas, estimando una inclinación orbital entre 50o y 55o. Teniendo en cuenta la importancia de obtener valores empíricos para las masas de estrellas O tempranas, y considerando la gran dispersión existente entre los valores observados y su discrepancia con los predichos por los modelos teóricos, hemos obtenido nuevas observaciones espectroscópicas de este sistema, con el propósito de mejorar los elementos orbitales derivados en la solución preliminar. Además, como las estrellas O tempranas suelen formar parte de cúmulos y asociaciones OB, hemos llevado a cabo una investigación espectroscópica de varias estrellas tempranas que podrían estar físicamente relacionadas con LSS~3074.

  13. Cool neutral hydrogen in the direction of an anonymous OB association

    SciTech Connect

    Bania, T.M.

    1983-08-01

    H I self-absorption is seen in the direction l = 55./sup 0/6 probably physically associated with an anonymous OB association which has the Cepheid GY Sagittae as a member. The cool H I is in two clouds at least 15 pc in diameter located 3.25 kpc from the Sun. If their temperature is approx. =50 K, the cloud masses are approx. =10/sup 3/ M/sub sun/. The neutral atomic hydrogen clouds are probably warm envelopes surrounding cold molecular cloud cores because CO observations in this region show two molecular clouds nearly coincident with the absorbing H i gas. Since the OB association is only approx. =10/sup 7/ years old, these clouds are likely to be part of the original cloud complex from which the stellar cluster formed. The H i clouds are part of the larger Arecibo survey of self-absorption which suggests that many of the Arecibo clouds are associated with heretofore unidentified star clusters. Even if this is generally not the case, the Arecibo objects have accurate kinematic distances and thus provide a new sample of cool H I clouds whose thermodynamic properties can be studied.

  14. Parametrization of DFTB3/3OB for Magnesium and Zinc for Chemical and Biological Applications

    PubMed Central

    2015-01-01

    We report the parametrization of the approximate density functional theory, DFTB3, for magnesium and zinc for chemical and biological applications. The parametrization strategy follows that established in previous work that parametrized several key main group elements (O, N, C, H, P, and S). This 3OB set of parameters can thus be used to study many chemical and biochemical systems. The parameters are benchmarked using both gas-phase and condensed-phase systems. The gas-phase results are compared to DFT (mostly B3LYP), ab initio (MP2 and G3B3), and PM6, as well as to a previous DFTB parametrization (MIO). The results indicate that DFTB3/3OB is particularly successful at predicting structures, including rather complex dinuclear metalloenzyme active sites, while being semiquantitative (with a typical mean absolute deviation (MAD) of ∼3–5 kcal/mol) for energetics. Single-point calculations with high-level quantum mechanics (QM) methods generally lead to very satisfying (a typical MAD of ∼1 kcal/mol) energetic properties. DFTB3/MM simulations for solution and two enzyme systems also lead to encouraging structural and energetic properties in comparison to available experimental data. The remaining limitations of DFTB3, such as the treatment of interaction between metal ions and highly charged/polarizable ligands, are also discussed. PMID:25178644

  15. A model for the infrared emission from an OB star cluster environment

    NASA Technical Reports Server (NTRS)

    Leisawitz, D.

    1991-01-01

    A model for the infrared emission from the neighborhood of an OB star cluster is described. The distribution of gas and dust around the stars, properties of the dust, and the cluster and interstellar radiation fields are variable. The model can be applied to regions around clusters embedded to various degrees in their parental molecular clouds (i.e., compact H II regions, blister-type H II regions, and the tenuous H II regions ionized by naked O stars). The model is used to simulate IRAS observations of a typical blister H II region. Infrared surface brightness and spectral energy distributions are predicted and the impact of limited spatial resolution is illustrated. The model results are shown to be consistent with observations of the exemplary outer Galaxy OB cluster NGC 7380. It is planned to use the model as a diagnostic tool to probe the physical conditions and dust properties in star-formation regions and, ultimately, in an interpretation of the spectral energy distributions of spiral galaxies.

  16. A CANDIDATE FOR THE MOST LUMINOUS OB ASSOCIATION IN THE GALAXY

    SciTech Connect

    Rahman, Mubdi; Matzner, Christopher; Moon, Dae-Sik

    2011-02-20

    The Milky Way harbors giant H II regions, which may be powered by star complexes more luminous than any known Galactic OB association. Being across the disk of the Galaxy, however, these brightest associations are severely extinguished and confused. We present a search for one such association toward the most luminous H II region in the recent catalog by Murray and Rahman, which, at {approx}9.7 kpc, has a recombination rate of {approx}7 x 10{sup 51} s{sup -1}. Prior searches have identified only small-scale clustering around the rim of this shell-like region, but the primary association has not previously been identified. We apply a near-infrared color selection and find an overdensity of point sources toward its southern central part. The colors and magnitudes of these excess sources are consistent with O- and early B-type stars at extinctions 0.96 < A{sub K} < 1.2, and they are sufficiently numerous (406 {+-} 102 after subtraction of field sources) to ionize the surrounding H II region, making this a candidate for the most luminous OB association in the Galaxy. We reject an alternate theory, in which the apparent excess is caused by localized extinction, as inconsistent with source demographics.

  17. Hard X-ray Flux from Low-Mass Stars in the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    Caramazza, M.; Drake, J. J.; Micela, G.; Flaccomio, E.

    2009-05-01

    We investigate the X-ray emission in the 20-40 keV band expected from the flaring low-mass stellar population in Cygnus OB2 assuming that the observed soft X-ray emission is due to a superposition of flares and that the ratio of hard X-ray to soft X-ray emission is described by a scaling found for solar flares by Isola and co-workers. We estimate a low-mass stellar hard X-ray flux in the 20-40 keV band in the range ~7×1031-7×1033 erg/s and speculate the limit of this values. Hard X-ray emission could lie at a level not much below the current observed flux upper limits for Cygnus OB2. Simbol-X, with its broad energy band (10-100 keV) and its sensitivity should be able to detect this emission and would provide insights into the hard X-ray production of flares on pre-main sequence stars.

  18. Interstellar NaI absorption towards the stellar association ARA OB1

    NASA Astrophysics Data System (ADS)

    Whiteoak, J. B.; Gardner, F. F.

    Observations have been made of the interstellar D-line absorption of Na I towards a loose grouping of OB stars (containing the association Ara OB1 and probably the H-alpha emission regions RCW 107 and 108) centered at 1 = 337.5 deg and b = - 0.5 deg. The individual absorption spectra contain several velocity components which show well-defined groupings at - 37, -18 and 0 km/s. The corresponding median optical depths are 2, 7 and 6. The evidence indicates that both the -37 and -18 km/s Na I clouds are located in the Carina-Sagittarius spiral arm. Several H II regions are located at similar longitudes, but most are close to zero latitude and are more distant than the arm. The association contains the galactic cluster NGC 61993. The results suggest that the turbulence seen optically towards this cluster has been caused by the interaction between two cloud groups moving towards each other at velocities of 10 to 15 km/s.

  19. Characterization of a young open cluster G144.9+0.4 in Cam OB1

    NASA Astrophysics Data System (ADS)

    Lin, Chien-Cheng; Chen, Wen-Ping; Panwar, Neelam

    2013-06-01

    Open clusters are good laboratories to study stellar evolution and dynamical interaction among member stars. We are carrying out a program to identify uncharted clusters using sky-survey data. Our preliminary result is finding stellar density enhancement using the 2MASS Point Source Catalog in Galactic latitude |b| < 50 deg. One such density peak (G144.9+0.4), located in the Cam OB1 association, is a young star cluster centered at (ℓ,b) = (144.904,+0.434), first recognized but not well characterized, by Glushkova et al. [8]. We used the PPMXL proper motions to constrain the membership. Our analysis yields a distance of 1.0 kpc. G144.9+0.4 is hence physically related to the Cam OB1 association. Based on the 2MASS and WISE colors, seven classical T-Tauri Star candidates are found within the cluster, though, three of these have proper motions inconsistent with members. One of the kinematic members has been confirmed by spectroscopy to be a CTTS.

  20. Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation.

    PubMed

    Sullivan, J P; Dickinson, D; Chase, H A

    1998-01-01

    One of the most problematic groups of the USEPA and EU priority pollutants are the halogenated organic compounds. These substances have a wide range of industrial applications, such as solvents and cleaners. Inadequate disposal techniques and accidental spillages have led to their detection in soil, groundwater, and river sediments. Persistence of these compounds in the environment has resulted from low levels of biodegradation due to chemical structural features that preclude or retard biological attack. Research has indicated the idea that treatment systems based on methanotrophic co-metabolic transformation may be a cost-effective and efficient alternative to physical methods because of the potential for high transformation rates, the possibility of complete compound degradation without the formation of toxic metabolites, applicability to a broad spectrum of compounds, and the use of a widely available and inexpensive growth substrate. A substantial amount of work concerning methanotrophic cometabolic transformations has been carried out using the soluble form of methane monooxygenase (sMMO) from the obligate methanotroph Methylosinus trichosporium OB3b. This NADH-dependent monooxygenase is derepressed when cells are grown under copper stress. sMMO has a wider specificity than the particulate form. sMMO has been shown to degrade trichloroethylene (TCE) at a rate of at least one order of magnitude faster than obtained with other mixed and pure cultures, suggesting it has a wider application to bioremediation. Furthermore, sMMO catalyzes an unusually wide range of oxidation reactions, including the hydroxylation of alkanes, epoxidation of alkenes, ethers, halogenated methanes, cyclic and aromatic compounds including compounds, that are resistant to degradation in the environment. However, the practical application of methantrophs and Methylosinus trichosporium OB3b to the treatment of chlorinated organics has met with mixed success. Although oxidation rates are

  1. Differential regulation of insulin receptor substrates-1 and -2 (IRS-1 and IRS-2) and phosphatidylinositol 3-kinase isoforms in liver and muscle of the obese diabetic (ob/ob) mouse.

    PubMed Central

    Kerouz, N J; Hörsch, D; Pons, S; Kahn, C R

    1997-01-01

    Intracellular insulin signaling involves a series of alternative and complementary pathways created by the multiple substrates of the insulin receptor (IRS) and the various isoforms of SH2 domain signaling molecules that can interact with these substrates. In this study, we have evaluated the roles of IRS-1 and IRS-2 in signaling to the phosphatidylinositol (PI) 3-kinase pathway in the ob/ob mouse, a model of the insulin resistance of obesity and non-insulin-dependent diabetes mellitus. We find that the levels of expression of both IRS-1 and IRS-2 are decreased approximately 50% in muscle, whereas in liver the decrease is significantly greater for IRS-2 (72%) than for IRS-1 (29%). This results in differential decreases in IRS-1 and IRS-2 phosphorylation, docking of the p85alpha regulatory subunit of PI 3-kinase, and activation of this enzyme in these two insulin target tissues. In ob/ob liver there is also a change in expression of the alternatively spliced isoforms of the regulatory subunits for PI 3-kinase that was detected at the protein and mRNA level. This resulted in a 45% decrease in the p85alpha form of PI 3-kinase, a ninefold increase in the AS53/p55alpha, and a twofold increase in p50alpha isoforms. Thus, there are multiple alterations in the early steps of insulin signaling in the ob/ob mouse, with differential regulation of IRS-1 and IRS-2, various PI 3-kinase regulatory isoforms, and a lack of compensation for the decrease in insulin signaling by any of the known alternative pathways at these levels. PMID:9399964

  2. Seismicity and Attenuation of the Continental Margin Off New York and Southern/ New England from OBS Data

    NASA Astrophysics Data System (ADS)

    Ten Brink, U. S.; Flores, C. H.; McGuire, J. J.; Collins, J. A.

    2014-12-01

    Earthquake data recorded during two ocean-bottom-seismometer (OBS) deployments on the continental slope off New York and Southern New England are used to evaluate micro-seismicity and attenuation of the continental margin. The deployments were partly motivated by the detection by land seismometers of several ML2.5-3.8 continental margin earthquakes in this region during the previous four years. The data come from a five-month-long cluster deployment (1 km radius) of eleven short-period OBS in July 2012 at a water depth of ~835 m and a subsequent nine-month deployment of a network of five short-period OBS spaced 50 km apart at water depths between 900-2400 m. The land-based seismic network did not identify seismic activity on the margin during the two deployments. The OBS network located only one earthquake of ML~1 near the shelf edge, suggesting that seismic activity of the margin is probably successfully monitored by land seismometers. A successful land-based detection of earthquakes on the margin may indicate that low seismic attenuation extends from the Eastern U.S. to the continental shelf and slope. To better quantify the attenuation of the margin we derive the peak spectral attenuation of 6-8 ML2.8-4.1 earthquakes recorded by the OBS during the two deployments. These earthquakes were located on land close to shore and offshore between New Jersey and Nova Scotia, and propagation paths are almost entirely within the continental margin. Onshore earthquakes of ML ≤2.6 were not detected by the OBS. Information about the seismicity and attenuation of the margin will be used to evaluate the probability of earthquake-generated landslides.

  3. Ocean Basalt Simulator version 1 (OBS1): Trace element mass balance in adiabatic melting of a pyroxenite-bearing peridotite

    NASA Astrophysics Data System (ADS)

    Kimura, Jun-Ichi; Kawabata, Hiroshi

    2015-01-01

    present a new numerical trace element mass balance model for adiabatic melting of a pyroxenite-bearing peridotite for estimating mantle potential temperature, depth of melting column, and pyroxenite fraction in the source mantle for a primary ocean basalt/picrite. The Ocean Basalt Simulator version 1 (OBS1) uses a thermodynamic model of adiabatic melting of a pyroxenite-bearing peridotite with experimentally/thermodynamically parameterized liquidus-solidus intervals and source mineralogy. OBS1 can be used to calculate a sequence of adiabatic melting with two melting models, including (1) melting of peridotite and pyroxenite sources with simple mixing of their fractional melts (melt-melt mixing model), and (2) pyroxenite melting, melt metasomatism in the host peridotite, and melting of the metasomatized peridotite (source-metasomatism model). OBS1 can be used to explore (1) the fractions of peridotite and pyroxenite, (2) mantle potential temperature, (3) pressure of termination of melting, (4) degree of melting, and (5) residual mode of the sources. In order to constrain these parameters, the model calculates a mass balance for 26 incompatible trace elements in the sources and in the generated basalt/picrite. OBS1 is coded in an Excel spreadsheet and runs with VBA macros. Using OBS1, we examine the source compositions and conditions of the mid-oceanic ridge basalts, Loihi-Koolau basalts in the Hawaiian hot spot, and Jurassic Shatsky Rise and Mikabu oceanic plateau basalts and picrites. The OBS1 model shows the physical conditions, chemical mass balance, and amount of pyroxenite in the source peridotite, which are keys to global mantle recycling.

  4. A Joint Monte Carlo Inversion of Seafloor Compliance, Receiver Functions and Rayleigh Wave Dispersion at OBS Stations off New Zealand

    NASA Astrophysics Data System (ADS)

    Ball, J. S.; Sheehan, A. F.; Stachnik, J. C.; Collins, J. A.

    2012-12-01

    Surface wave dispersion measurements, receiver functions and seafloor compliance data were jointly inverted to produce shear velocity profiles at Ocean Bottom Seismic (OBS) stations off the South Island of New Zealand. Receiver function analysis of OBS data presents a technical challenge due largely to the presence of multiply scattered reverberations in the water and low-velocity sediments at OBS sites. Teleseismic arrivals from interfaces of tectonic interest are obscured by sediment-converted shear waves and water column multiples. Mitigation of these effects typically requires accurate characterization of sediment layer shear velocities and thicknesses. The seismic properties of the sediment column can be probed using shallow mode-converted shear arrivals and the seafloor's response to ocean loading from infragravity waves (seafloor compliance). Data for this study were acquired in 2009-2010 by the Marine Observations of Anisotropy Near Aotearoa (MOANA) experiment, which deployed 30 broadband OBS and differential pressure gauges (DPGs) off the South Island of New Zealand. Seafloor compliance was calculated from MOANA DPG pressure and OBS vertical component acceleration power spectra at periods between 50-250s. The resulting compliance values range from 10-10-10-8 Pa-1 and are sensitive to changes in shear modulus at depths that increase with forcing period. Receiver functions were estimated using events of high SNR from epicentral distances of 30-90 degrees. Forward modeling utilized full-wavefield synthetic seismograms to account for the OBS location at the boundary between acoustic and elastic media. Rayleigh wave group velocity dispersion curves at periods from 6-30s were derived from ambient noise tomography. A Bayesian Markov Chain Monte Carlo algorithm was implemented to estimate shear velocity profiles, crustal and sediment thicknesses using these combined observations. Preliminary sediment thickness estimates range from 400m on the Challenger plateau to

  5. VizieR Online Data Catalog: Cep OB2 Herschel/PACS view (Sicilia-Aguilar+, 2015)

    NASA Astrophysics Data System (ADS)

    Sicilia-Aguilar, A.; Roccatagliata, V.; Getman, K.; Riviere-Marichalar, P.; Birnstiel, T.; Merin, B.; Fang, M.; Henning, T.; Eiroa, C.; Currie, T.

    2015-02-01

    The Cep OB2 clusters, Tr 37 (centered at 21:38:09, +57:26:48, J2000) and NGC 7160 (centered at 21:53:40, 62:36:10, J2000), were observed with the ESA Herschel Space Observatory using the Photodetector Array Camera and Spectrometer (PACS), as part of the open time (OT) program "Disk dispersal in Cep OB2" (PI A. Sicilia-Aguilar). We obtained a total of 23h observing time in parallel mode at 70 and 160um. Observations took place between November 2012 and January 2013. (4 data files).

  6. MARS - CheObs ed. -- A flexible Software Framework for future Cherenkov Telescopes

    NASA Astrophysics Data System (ADS)

    Bretz, T.; Dorner, D.

    2010-04-01

    In gamma-ray astronomy, a new ground-based project named DWARF (Dedicated multiWavelength Agn Research Facility) is entering the field. It is a Cherenkov telescope project aimed at long-term monitoring of the brightest AGNs in the TeV energy range. One of the former HEGRA telescopes is being refurbished and upgraded with a Geigermode-APD camera. It is planned to be operated as a robotic telescope on the Canary Island of La Palma. Using new technologies, an improvement in sensitivity and an energy threshold of 400GeV are expected. Future plans foresee more small Cherenkov telescopes around the globe enabling for the first time 24 h monitoring in the VHE range. Long-term observations of the brightest AGNs provide the possibility to search for orbital modulation of blazar emission due to super-massive black hole binaries, to study the statistics of flares and their physical origin, and to correlate the data with corresponding data from the neutrino observatory IceCube to search for evidence of hadronic emission processes. For this project, a flexible and user friendly software package is available: Modular Analysis and Reconstruction Software - Cherenkov Observatory edition (MARS - CheObs ed.). The package provides a framework for any event-based analysis. For the application in the Imaging Air Cherenkov Technique, various methods and algorithms are available. Currently, it is being used for the MAGIC telescope. To allow for automatic analysis, MARS - CheObs ed. includes an automation concept which allows not only for automatic processing of the data, but also for automatic production of simulated data. For the DWARF project, a simulation program (ceres) has been developed and included in the software package. Using this, a design study for the technical upgrades of the telescope was performed. The simulation of the showers in the atmosphere is performed using the CORSIKA package. The output of this is fed into the telescope simulation ceres. Proper simulations are

  7. Trace metals in the Ob and Yenisei Rivers' Estuaries (the Kara Sea).

    NASA Astrophysics Data System (ADS)

    Demina, L. L.

    2014-12-01

    Behavior of some trace metals (Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni and Pb) in water column (soluble <0.45 µm and particulate fractions) and bottom sediments (surface and cores) along the two transects from the Ob River and Yenisei River Estuaries to the Kara Sea was studied. The length of both transects was about 700 km. Water depth was 12-63 m, O2 dissolved :5.36-9.55 ml l-1. Along the transects salinity increased from 0.07 to 34.2 psu, while the SPM' concentration decreased from 10.31 to 0.31 mg/l. Total suspended particulate matter load is more than one order of magnitude higher in the Ob River Estuary comparing to that of the Yenisei River. It has led to a significant difference between the suspended trace metals' concentrations (µg/l) in water of the two estuaries. With salinity increase along transects Fe susp., Mn susp. and Zn susp. decreased by a factor of 100-500, that has led to a growth of a relative portion of dissolved trace metals followed by their bioaccumulation (Demina et al., 2010). A strong direct correlation between suspended Cu, Fe and SPM mass concentration was found. For the first time along the Yenisei River' Estuary -the Kara Sea transect a direct positive correlation between Cu suspended and volume concentration of SPM (mg/ml3) was found, that was attributed to contribution of phytoplankton aggregates in the SPM composition. A trend of relationship between content of suspended As and pelitic fraction (2-10 µm) of SPM was firstly found in theses basins also. Study of trace metal speciation in the bottom sediments (adsorbed, associated with Fe-Mn (oxyhydr)oxides, organic matter and fixed in the mineral lattice or refractory) has revealed the refractory fraction to be prevailing (70-95% total content) for Fe, Zn, Cu, Co, Ni, Cr, Cd and Pb. That means that toxic heavy metals were not available for bottom fauna. Mn was predominantly found in the adsorbed and (oxyhydr)oxides geochemically labile forms, reflecting the redox condition change

  8. Earth's crust model of the South-Okhotsk Basin by wide-angle OBS data

    NASA Astrophysics Data System (ADS)

    Kashubin, Sergey N.; Petrov, Oleg V.; Rybalka, Alexander V.; Milshtein, Evgenia D.; Shokalsky, Sergey P.; Verba, Mark L.; Petrov, Evgeniy O.

    2017-07-01

    Deep seismic studies of the Sea of Okhotsk region started in late 1950s. Since that time, wide-angle reflection and refraction data on more than two dozen profiles were acquired. Only five of those profiles either crossed or entered the deep-water area of the South-Okhotsk Basin (also known as the Kuril Basin or the South-Okhotsk Deep-Water Trough). Only P-waves were used to develop velocity-interface models in all the early research. Thus, all seismic and geodynamic models of the Okhotsk region were based only on the information on compressional waves. Nevertheless, the use of Vp/Vs ratio in addition to P-wave velocity allows discriminating felsic and mafic crustal layers with similar Vp values. In 2007 the Russian seismic service company Sevmorgeo acquired multi-component data with ocean bottom seismometers (OBS) along the 1700-km-long north-south 2-DV-M Profile. Only P-wave information was used previously to develop models for the entire profile. In this study, a multi-wave processing, analysis, and interpretation of the OBS data are presented for the 550-km-long southern segment of this Profile that crosses the deep-water South-Okhotsk Basin. Within this segment 50 seismometers were deployed with nominal OBS station spacing of 10-12 km. Shot point spacing was 250 m. Not only primary P-waves and S-waves but also multiples and P-S, S-P converted waves were analyzed in this study to constrain velocity-interface models by means of travel time forward modeling. In offshore deep seismic studies, thick water layer hinders an estimation of velocities in the sedimentary cover and in the upper consolidated crust. Primarily, this is due to the fact that refracted waves propagating in low-velocity solid upper layers interfere with high-amplitude direct water wave. However, in multi-component measurements with ocean bottom seismometers, it is possible to use converted and multiple waves for velocity estimations in these layers. Consequently, one can obtain P- and S

  9. First Results From the SAGER-OBS Deep Seismic Cruise (July/August 2006) Offshore Sumatra

    NASA Astrophysics Data System (ADS)

    Klingelhoefer, F.; Dessa, J.; Permana, H.; Graindorge, D.; Dean, S.; White, N.; Carton, H.; Singh, S.; Aouji, O.; Aryawan, K. G.; Begot, J.; Beguery, L.; Burchell, A.; Chaubey, A. K.; Chauhan, A.; Crozon, J.; Daniel, R.; Fernagu, P.; Galih, D. R.; Greenroyd, C. J.; Laesanpura, A.; Pelleau, P.; Prihantono, J.; Royle, G.; Shankar, U.

    2006-12-01

    The 26th December 2004 great Sumatra earthquake (Mw=9.1) is among the 4 largest earthquakes ever recorded and the largest of the last 40~years. It initiated at a depth of 20-30~km and ruptured about 1300~km of the Indo-Australian/Sunda plate boundary, from the vicinity of Simeulue Island up to the north of Andaman Islands. The SAGER program is part of an ongoing international effort to study the rupture zone of this great earthquake. The main objective of the SAGER-OBS cruise on board R/V Marion Dufresne (July 7th to August 9th 2006) was the acquisition of deep penetrating wide-angle seismic data to constrain the crustal and upper mantle structures in the epicentral area and in the zone of greatest co-seismic slip. Forward and inverse modeling of the data will help to identify potential heterogeneities along the subduction thrust, such as subducted topographical features, to obtain the velocity structure within the subducting lithosphere and overlying deep fore-arc crustal zones. The cruise was performed simultaneously with the acquisition of coincident reflection seismic profiles by the Western-Geco/Schlumberger's "Searcher" seismic vessel on the same profiles (Singh et al., 2006). During the cruise, 56 ocean-bottom seismometers (OBS) were deployed successively along two profiles located north of Simeulue Island (250~km length) and north of Sumatra, close to the limit of Indonesian waters (520~km-long profile). The two profiles are sub-parallel (oriented NE-SW), roughly perpendicular to the margin, and extend well onto the oceanic basin; the northern one crosses the submerged arc north of Sumatra. Seismic shots along the profiles were generated using a 8260~cu inch air-gun array. All OBS's were successfully recovered. The resulting data quality is good with deep penetrating arrivals on most of the instruments. Additionally, shots fired by the second vessel while acquiring the coincident reflection profiles were recorded by the instruments deployed along the

  10. Magnetic field measurements and wind-line variability of OB-type stars

    NASA Astrophysics Data System (ADS)

    Schnerr, R. S.; Henrichs, H. F.; Neiner, C.; Verdugo, E.; de Jong, J.; Geers, V. C.; Wiersema, K.; van Dalen, B.; Tijani, A.; Plaggenborg, B.; Rygl, K. L. J.

    2008-06-01

    Context: The first magnetic fields in O- and B-type stars that do not belong to the Bp-star class, have been discovered. The cyclic UV wind-line variability, which has been observed in a significant fraction of early-type stars, is likely to be related to such magnetic fields. Aims: We attempt to improve our understanding of massive-star magnetic fields, and observe twenty-five carefully-selected, OB-type stars. Methods: Of these stars we obtain 136 magnetic field strength measurements. We present the UV wind-line variability of all selected targets and summarise spectropolarimetric observations acquired using the MUSICOS spectropolarimeter, mounted at the TBL, Pic du Midi, between December 1998 and November 2004. From the average Stokes I and V line profiles, derived using the LSD method, we measure the magnetic field strengths, radial velocities, and first moment of the line profiles. Results: No significant magnetic field is detected in any OB-type star that we observed. Typical 1σ errors are between 15 and 200 G. A possible magnetic-field detection for the O9V star 10 Lac remains uncertain, because the field measurements depend critically on the fringe-effect correction in the Stokes V spectra. We find excess emission in UV-wind lines, centred about the rest wavelength, to be a new indirect indicator of the presence of a magnetic field in early B-type stars. The most promising candidates to host magnetic fields are the B-type stars δ Cet and 6 Cep, and a number of O stars. Conclusions: Although some O and B stars have strong dipolar field, which cause periodic variability in the UV wind-lines, such strong fields are not widespread. If the variability observed in the UV wind-lines of OB stars is generally caused by surface magnetic fields, these fields are either weak (⪉few hundred G) or localised. Figures [see full textsee full textsee full textsee full textsee full textsee full text]-[see full textsee full textsee full textsee full textsee full textsee

  11. Modulation of murine bone marrow-derived CFU-F and CFU-OB by in vivo bisphosphonate and fluoride treatments.

    PubMed

    Chou, M-Y; Yan, D; Jafarov, T; Everett, E T

    2009-05-01

    Bisphosphonates (BPN) have actions on a variety of cell types including: osteoclasts, osteoblasts, osteocytes, and endothelial cells. The objectives of this report are to review the current state of understanding of the effects of BPNs on orthodontic tooth movement and to provide evidence on BPN's in vivo effects on bone marrow-derived osteoprogenitor cells. Mice from the C3H/HeJ (C3H), C57BL/6J (B6), FVB/NJ (FVB), and BALB/cByJ (BALB) strains were treated for 3 weeks with 0, 3, 30, or 150 mcg/kg/week alendronate (ALN) administered subcutaneous alone or in combination with 50 ppm fluoride (F). Bone marrow cells were harvested and subjected to in vitro colony-forming unit fibroblast (CFU-F) and colony-forming unit osteoblasts (CFU-OB) assays. Baseline differences in CFU-F, CFU-OB/ALP+, and CFU-OB/total were observed among the four strains. Strain-specific responses to ALN and F treatments were observed for CFU-F, CFU-OB/ALP+, and CFU-OB/total. F treatment alone resulted in decreases in CFU-F (p = 0.013), CFU-OB/ALP+ (p = 0.005), and CFU-OB/total (p = 0.003) in the C3H strain. CFU-F (p = 0.036) were decreased by F in the B6 strain. No significant (NS) effects of F were observed for FVB and BALB. ALN treatment resulted in a significant decrease in CFU-F (p = 0.0014) and CFU-OB/total (p = 0.028) in C3H only. ALN treatment had NS effect on CFU-OB/ALP+ in all four strains. Genetic factors appear to play a role in ALN's effects on CFU-F and CFU-OB/total but not on CFU-OB/ALP+.

  12. A photometric study of the Orion OB 1 association. 1: Observational data

    NASA Technical Reports Server (NTRS)

    Warren, W. H., Jr.; Hesser, J. E.

    1976-01-01

    An extensive catalog of observational data is presented for stars in the region of the young stellar association Orion OB 1. In addition to new photoelectric observations obtained on the uvbyB and UBV systems, photoelectric and spectroscopic data were compiled for the stars observed and for several bright members of the association having available photometric indices. Mean weighted values were computed for the uvbyB and UBV data and are tabulated in summary tables which include all references for individual values. These tables are expected to be reasonably complete for association members earlier than spectral type A0. From an analysis of currently available proper motion, radial velocity, and photometric data, membership criteria were derived and qualitative membership probabilities for 526 stars were summarized. A set of charts is included for assistance in identification of the program stars in all regions of the association.

  13. Islet adaptation to obesity and insulin resistance in WNIN/GR-Ob rats.

    PubMed

    Singh, Himadri; Ganneru, Sireesha; Malakapalli, Venkata; Chalasani, Maniprabha; Nappanveettil, Giridharan; Bhonde, Ramesh R; Venkatesan, Vijayalakshmi

    2014-01-01

    WNIN/GR-Ob mutant rat is a novel animal model to study metabolic syndrome (obesity, insulin resistance, hyperinsulinemia, impaired glucose tolerance and cardiovascular diseases). We have investigated the islet characteristics of obese mutants at different age groups (1, 6 and 12 months) to assess the islet changes in response to early and chronic metabolic stress. Our data demonstrates altered islet cell morphology and function (hypertrophy, fibrotic lesions, vacuolation, decreased stimulation index, increased TNFα, ROS and TBARS levels) in mutants as compared to controls. Furthermore, network analysis (gene-gene interaction) studied in pancreas demonstrated increased inflammation as a key factor underlying obesity/metabolic syndrome in mutants. These observations pave way to explore this model to understand islet adaptation in response to metabolic syndrome.

  14. Modeling the Radio Emission from Cyg OB2 No. 5: A Quadruple System?

    NASA Astrophysics Data System (ADS)

    Kennedy, M.; Dougherty, S. M.; Fink, A.; Williams, P. M.

    2010-02-01

    Fifty observations at frequencies between 1.4 GHz and 43 GHz of the 6.6 day O6.5-7+O5.5-6 binary Cyg OB2 No. 5 using the Very Large Array over 20 years are re-examined. The aim is to determine the location and character of the previously detected variable radio emission. The radio emission from the system consists of a primary component that is associated with the binary, and a non-thermal source (NE), 0farcs8 to the NE of the binary that has been ascribed to a wind-collision region (WCR) between the stellar winds of the binary and that of a B-type star (Star D) to the NE. Previous studies have not accounted for the potential contribution of NE to the total radio emission, most especially in observations where the primary and NE sources are not resolved as separate sources. NE shows no evidence of variation in 23 epochs where it is resolved separately from the primary radio component, demonstrating that the variable emission arises in the primary component. Since NE is non-variable, the radio flux from the primary can now be well determined for the first time, most especially in observations that do not resolve both the primary and NE components. The variable radio emission from the primary component has a period of 6.7 ± 0.3 years which is described by a simple model of a non-thermal source orbiting within the stellar wind envelope of the binary. Such a model implies the presence of a third, unresolved stellar companion (Star C) orbiting the 6.6 day binary with a period of 6.7 years and independent of Star D to the NE. The variable non-thermal emission arises from either a WCR between Star C and the binary system, or possibly from Star C directly. The model gives a mass-loss rate of 3.4 × 10-5 M sun yr-1 for Cyg OB2 No. 5, unusually high for an Of supergiant and comparable to that of WR stars, and consistent with an unusually strong He I 1.083 µm emission line, also redolent of WR stars. An examination of radial velocity observations available from the

  15. A FORMATION SCENARIO OF YOUNG STELLAR GROUPS IN THE REGION OF THE SCORPIO CENTAURUS OB ASSOCIATION

    SciTech Connect

    Ortega, V. G.; Jilinski, E.; De la Reza, R.; Bazzanella, B.

    2009-04-15

    The main objective of this work is to investigate the role played by Lower Centaurus Crux (LCC) and Upper Centaurus Lupus (UCL), both subcomponents of the Scorpio Centaurus OB association (Sco-Cen), in the formation of the groups {beta} Pictoris, TW Hydrae, and the {eta} Chamaeleontis cluster. The dynamical evolution of all the stellar groups involved and of the bubbles and shells blown by LCC and UCL are calculated, and followed from the past to the present. This leads to a formation scenario in which (1) the groups {beta} Pictoris, TW Hydrae were formed in the wake of the shells created by LCC and UCL, (2) the young cluster {eta} Chamaeleontis was born as a consequence of the collision of the shells of LCC and UCL, and (3) the formation of Upper Scorpius (US), the other main subcomponent of the Sco-Cen association, may have been started by the same process that created {eta} Chamaeleontis.

  16. Search for old neutron stars in molecular clouds: Cygnus rift and Cygnus OB7.

    NASA Astrophysics Data System (ADS)

    Belloni, T.; Zampieri, L.; Campana, S.

    1997-03-01

    We present the results of a systematic search for old isolated neutron stars (ONSs) in the direction of two giant molecular clouds in Cygnus (Rift and OB7). From theoretical calculations, we expect the detection of a large number of ONSs with the PSPC on board ROSAT. By analyzing the PSPC pointings in the direction of the clouds, we find four sources characterized by count rates (~10^-3^ct/s) and spectral properties consistent with the hypothesis that the X-ray radiation is produced by ONSs and also characterized by the absence of any measurable optical counterpart within their error circle in the digitized red plates of the Palomar All Sky Survey. The importance of follow-up deep observations in the direction of these ONS candidates is discussed. The observational and theoretical approach presented here could be fruitfully applied also to the systematic search for ONSs in other regions of the Galaxy.

  17. MODELING THE RADIO EMISSION FROM Cyg OB2 NO. 5: A QUADRUPLE SYSTEM?

    SciTech Connect

    Kennedy, M.; Dougherty, S. M.; Fink, A.; Williams, P. M. E-mail: sean.dougherty@nrc.c E-mail: pmw@roe.ac.u

    2010-02-01

    Fifty observations at frequencies between 1.4 GHz and 43 GHz of the 6.6 day O6.5-7+O5.5-6 binary Cyg OB2 No. 5 using the Very Large Array over 20 years are re-examined. The aim is to determine the location and character of the previously detected variable radio emission. The radio emission from the system consists of a primary component that is associated with the binary, and a non-thermal source (NE), 0.''8 to the NE of the binary that has been ascribed to a wind-collision region (WCR) between the stellar winds of the binary and that of a B-type star (Star D) to the NE. Previous studies have not accounted for the potential contribution of NE to the total radio emission, most especially in observations where the primary and NE sources are not resolved as separate sources. NE shows no evidence of variation in 23 epochs where it is resolved separately from the primary radio component, demonstrating that the variable emission arises in the primary component. Since NE is non-variable, the radio flux from the primary can now be well determined for the first time, most especially in observations that do not resolve both the primary and NE components. The variable radio emission from the primary component has a period of 6.7 +- 0.3 years which is described by a simple model of a non-thermal source orbiting within the stellar wind envelope of the binary. Such a model implies the presence of a third, unresolved stellar companion (Star C) orbiting the 6.6 day binary with a period of 6.7 years and independent of Star D to the NE. The variable non-thermal emission arises from either a WCR between Star C and the binary system, or possibly from Star C directly. The model gives a mass-loss rate of 3.4 x 10{sup -5} M{sub sun} yr{sup -1} for Cyg OB2 No. 5, unusually high for an Of supergiant and comparable to that of WR stars, and consistent with an unusually strong He I 1.083 mum emission line, also redolent of WR stars. An examination of radial velocity observations available

  18. Les aspects psychosociaux de l’obésité chez les enfants et les adolescents

    PubMed Central

    Nieman, Peter; LeBlanc, Claire MA

    2012-01-01

    RÉSUMÉ En plus de donner des conseils aux familles au sujet de l’activité physique régulière et d’une saine alimentation, les cliniciens doivent déterminer les facteurs psychosociaux qui contribuent à l’obésité des enfants ou des adolescents et les aider à y faire face. Les personnes touchées peuvent souffrir de dépression, de mauvaise estime de soi, d’intimidation et de préjugés liés au poids, qui sont tous des expériences qui peuvent compliquer l’obtention des résultats de santé souhaités. Les cliniciens devraient tenter de déterminer les facteurs stressants sous-jacents et s’assurer de la mise en œuvre de conseils pertinents.

  19. A photometric study of the Orion OB 1 association. I - Observational data. II - Photometric analysis

    NASA Technical Reports Server (NTRS)

    Warren, W. H., Jr.; Hesser, J. E.

    1977-01-01

    A catalog of observational data is presented for stars in the region of the young stellar association Orion OB 1. Photoelectric observations obtained in the uvby-beta and UBV systems are compiled along with previous photoelectric and spectroscopic data for all these stars as well as for several bright members of the association with available photometric indices. Mean weighted values are computed for the photometric data and summarized in tables expected to be reasonably complete for association members earlier than spectral type A0. Membership criteria are derived, and qualitative membership probabilities summarized, for the 526 stars in the final program. The analytical procedures are discussed for association stars of B, intermediate, and AF types. Effects of the nebular environment and various calibrations of Balmer-line and four-color indices are considered for the determination of absolute magnitudes for the B-type stars.

  20. A Catalog of OB Associations in the spiral galaxy NGC 300

    NASA Astrophysics Data System (ADS)

    Pietrzyński, G.; Gieren, W.; Fouqué, P.; Pont, F.

    2001-05-01

    We present results of a search for OB associations in NGC 300. Using an automatic and objective method (PLC technique) 117 objects were found. Statistical tests indicate that our sample is contaminated by less than 10 detections due to random concentrations of blue stars. Spatial distributions of detected associations and H II regions are strongly correlated. The size distribution reveals a significant peak at about 60 mu rad which corresponds to 125 parsecs if a distance modulus of 26.66 mag is assumed. Besides the objects with sizes corresponding to typical associations we also found several much larger objects. A second level application of our detection method revealed that most of these are composed of smaller subgroups, with sizes of about 100 pc. Based in part on observations obtained with the 1.3~m Warsaw telescope at the Las Campanas Observatory of the Carnegie Institution of Washington, and on observations obtained at the European Southern Observatory, La Silla, Chile.

  1. BOREAS RSS-17 Xylem Flux Density Measurements at the SSA-OBS Site

    NASA Technical Reports Server (NTRS)

    Zimmerman, Reiner; Way, JoBea; McDonald, Kyle; Nickeson, Jaime (Editor); Hall, Forrest G. (Editor); Smith, David E. (Technical Monitor)

    2000-01-01

    As part of its efforts to determine environmental and phenological states from radar imagery, the Boreal Ecosystem-Atmosphere Study (BOREAS) Remote Sensing Science (RSS)-17 team collected in situ tree xylem flow measurements for one growing season on five Picea mariana (black spruce) trees. The data were collected to obtain information on the temporal and spatial variability in water uptake by trees in the Southern Study Area-Old Black Spruce (SSA-OBS) stand in the BOREAS SSA. Temporally, the data were collected in 30-minute intervals for 120 days from 31 May 1994 until 27 September 1994. The data are stored in tabular ASCII files. The xylem flux data are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). The data files are available on a CD-ROM (see document number 20010000884).

  2. Low energy gamma ray emission from the Cygnus OB2 association

    NASA Technical Reports Server (NTRS)

    Chen, Wan; White, Richard L.

    1992-01-01

    According to our newly developed model of gamma-ray emission from chaotic early-type stellar winds, we predict the combined gamma-ray flux from the circumstellar winds of many very luminous early-type stars in the Cyg OB2 association can be detectable by the Energetic Gamma Ray Experiment Telescope (EGRET) (and maybe also by OSSE) on CGRO. Due to different radiation mechanisms, the gamma-ray spectrum from stellar winds can be quite different from that of CYG X-3; this spectral difference and the time-variation of Cyg X-3 flux will help to distinguish the gamma-ray components from different sources in this small region, which is spatially unresolvable by CGRO.

  3. A new composite assembly mechanism for supporting QoS in OBS networks

    NASA Astrophysics Data System (ADS)

    Guan, Ai-hong; Hu, Fang; Li, Wan-chong

    2014-01-01

    To provide the differential quality of service (QoS) for different classes of packets and reduce the packet loss probability (PLP), a novel priority-based composite assembly scheme for optical burst switching (OBS) networks is proposed. The low and high packet classes are aggregated into a single burst simultaneously, and the highest-priority packets are placed in the middle, while the low-priority packets are at the tail and head of the burst. The priority is lowered gradually from the middle to the ends. Simulation results demonstrate that the proposed assembly strategy not only guarantees the integrity of the high-priority bursts, but also significantly reduces the average end-to-end delay of the bursts and the PLP of network. So it can adapt to the flexible network with QoS requirement.

  4. OB associations and the nonuniversality of the cosmic abundances - Implications for cosmic rays and meteorites

    NASA Technical Reports Server (NTRS)

    Olive, K. A.; Schramm, D. N.

    1982-01-01

    The formation of the solar system inside an OB association is examined with particular attention to the elemental abundances which would have been ejected by the association's first few supernovae. It is found that the solar system material may have been significantly contaminated by these supernovae and thus the average interstellar composition may differ from the solar system composition. In particular, we find that many of the so-called isotopic and elemental abundance anomalies (e.g., Ne, C, O, s-process/r-process, etc.) found in meteoritic inclusions and in cosmic rays may be more representative of the average interstellar abundance. In other words, it may be that the average solar system abundances are what is 'anomalous'.

  5. Ultraviolet Imaging Telescope photometry of massive stars - The OB association NGC 206 in M31

    NASA Technical Reports Server (NTRS)

    Hill, Jesse K.; Pfarr, Barbara B.; Bohlin, Ralph C.; Isensee, Joan E.; O'Connell, Robert W.; Neff, Susan G.; Roberts, Morton S.; Smith, Andrew M.; Stecher, Theodore P.

    1992-01-01

    The Ultraviolet Imaging Telescope (UIT) obtained UV images of the giant M31 OB association NGC 206. Magnitudes in bands at 1520 and 2490 A were obtained for 30 massive stars, which demonstrate the effectiveness of UIT for photometry of moderately crowded hot stars to V about 21. The UV colors and magnitudes observed for stars in NGC 206 place them in the region of the color magnitude diagram occupied by evolutionary models for 30-60 solar mass stars, after correcting for extinction. The brighter stars are systematically redder than the fainter stars, indicating that they are supergiants of age about 4 Myr, while the fainter, bluer stars are nearer age zero. The relative numbers of probable supergiants measured by us and the number of probable main-sequence O stars measured from optical images are in agreement with the relative lifetimes. Calculated UIT colors are presented for a library of standard star spectra constructed from IUE and ground-based observations.

  6. Orion's Cloak - A rapidly expanding shell of gas centered on the Orion OB1 association

    NASA Technical Reports Server (NTRS)

    Cowie, L. L.; Songaila, A.; York, D. G.

    1979-01-01

    The structure of the interstellar gas surrounding the Orion OB1 association and the neighboring lambda Orionis association is detailed. UV absorption lime spectra of various ionization stages of C, N, Si and S in the directions of 12 stars were obtained by means of the spectrometer on board the Copernicus satellite. The presence of a shell of material surrounding the two associations and expanding at 100 to 120 km/sec, designated Orion's Cloak, was revealed, together with sporadically occurring higher column density matter at lower velocities. Results are interpreted to indicate the presence of a rapidly moving radiative shock outside the H II region of the association stars and inside this feature, a lower velocity, higher column density cloud which appears to be directly ionized by association stars. It is suggested that the gas features are caused by the effects of a recent supernova and of multiple supernovae, stellar winds and rocket-accelerated clouds in addition to stellar ionization.

  7. Present and future gamma-ray probes of the Cygnus OB2 environment

    SciTech Connect

    Anchordoqui, Luis A.; Goldberg, Haim; Moore, Russell D.; Palomares-Ruiz, Sergio; Torres, Diego F.; Weiler, Thomas J.

    2009-11-15

    The MAGIC Collaboration has provided new observational data pertaining to the TeV J2032+4130 gamma-ray source (within the Cygnus OB2 region), for energies E{sub {gamma}}>400 GeV. It is then appropriate to update the impact of these data on gamma-ray production mechanisms in stellar associations. We consider two mechanisms of gamma-ray emission, pion production and decay (PION) and photoexcitation of high-energy nuclei followed by prompt photoemission from the daughter nuclei (A*). We find that while the data can be accommodated with either scenario, the A* features a spectral bump, corresponding to the threshold for exciting the giant dipole resonance, which can serve to discriminate between them. We comment on neutrino emission and detection from the region if the PION and/or A* processes are operative. We also touch on the implications for this analysis of future Fermi and Cerenkov Telescope array data.

  8. Kinematics of the galaxy from OB stars with proper motions from the Gaia DR1 catalogue

    NASA Astrophysics Data System (ADS)

    Bobylev, V. V.; Bajkova, A. T.

    2017-03-01

    We consider two samples of OB stars with different distance scales that we have studied previously. The first and second samples consist of massive spectroscopic binaries with photometric distances and distances determined from interstellar calcium lines, respectively. The OB stars are located at heliocentric distances up to 7 kpc. We have identified them with the Gaia DR1 catalogue. Using the proper motions taken from the Gaia DR1 catalogue is shown to reduce the random errors in the Galactic rotation parameters compared to the previously known results. By analyzing the proper motions and parallaxes of 208 OB stars from the Gaia DR1 catalogue with a relative parallax error of less than 200%, we have found the following kinematic parameters: ( U, V)⊙ = (8.67, 6.63)± (0.88, 0.98) km s-1, Ω0 = 27.35 ± 0.77 km s-1 kpc-1, Ω'0 = -4.13 ± 0.13 km s-1 kpc-2, and Ω″0 = 0.672 ± 0.070 km s-1 kpc-3, the Oort constants are A = -16.53 ± 0.52 km s-1 kpc-1 and B = 10.82 ± 0.93 km s-1 kpc-1, and the linear circular rotation velocity of the local standard of rest around the Galactic rotation axis is V 0 = 219 ± 8 km s-1 for the adopted R 0 = 8.0 ± 0.2 kpc. Based on the same stars, we have derived the rotation parameters only from their line-of-sight velocities. By comparing the estimated values of Ω'0, we have found the distance scale factor for the Gaia DR1 catalogue to be close to unity: 0.96. Based on 238 OB stars of the combined sample with photometric distances for the stars of the first sample and distances in the calcium distance scale for the stars of the second sample, line-of-sight velocities, and proper motions from the Gaia DR1 catalogue, we have found the following kinematic parameters: ( U, V, W)⊙ = (8.19, 9.28, 8.79)± (0.74, 0.92, 0.74) km s-1, Ω0 = 31.53 ± 0.54 km s-1 kpc-1, Ω'0 = -4.44 ± 0.12 km s-1 kpc-2, and Ω″0 = 0.706 ± 0.100 km s-1 kpc-3; here, A = -17.77 ± 0.46 km s-1 kpc-1, B = 13.76 ± 0.71 km s-1 kpc-1, and V 0 = 252 ± 8 km s-1.

  9. Nonalcoholic steatohepatitis (NASH) in ob/ob mice treated with yo jyo hen shi ko (YHK): effects on peroxisome proliferator-activated receptors (PPARs) and microsomal triglyceride transfer protein (MTP).

    PubMed

    Stefano, José Tadeu; de Oliveira, Claudia Pinto Marques Souza; Corrêa-Giannella, Maria Lúcia; de Lima, Vicência Mara Rodrigues; de Sá, Sandra Valéria; de Oliveira, Ellen Pierre; de Mello, Evandro Sobroza; Giannella-Neto, Daniel; Alves, Venâncio Avancini Ferreira; Carrilho, Flair José

    2007-12-01

    YHK has antioxidant properties, has a hypoglycemic effect, and may reduce plasma lipid levels. In this study, we examined the hepatic expression of PPAR-alpha and -gamma and MTP in ob/ob mice receiving or not receiving YHK. Ob/ob mice were assigned to receive oral YHK (20 mg/kg/day) fed solution (methionine/choline-deficient [MCD] diet+YHK group) or vehicle (MCD group) by gavage for 4 weeks. Liver fragments were collected for histologic examination and mRNA isolation. PPAR-alpha and -gamma and MTP gene expression was examined by RT-qPCR. YHK treatment was associated with NASH prevention, weight loss, and reduction of visceral fat and of serum concentrations of aminotransferases in comparison to the MCD group. YHK promoted an increment in PPAR-alpha and MTP and a decrement in PPAR-gamma mRNA contents. These findings suggest that modulation of PPAR-alpha and -gamma and MTP RNA expression may be implicated in the protective effect of YHK in experimental NASH, limiting hepatocyte lipid accumulation.

  10. Proper motions of five OB stars with candidate dusty bow shocks in the Carina Nebula

    NASA Astrophysics Data System (ADS)

    Kiminki, Megan M.; Smith, Nathan; Reiter, Megan; Bally, John

    2017-06-01

    We constrain the proper motions of five OB stars associated with candidate stellar wind bow shocks in the Carina Nebula using Hubble Space Telescope ACS imaging over 9-10 yr baselines. These proper motions allow us to directly compare each star's motion to the orientation of its candidate bow shock. Although these stars are saturated in our imaging, we assess their motion by the shifts required to minimize residuals in their airy rings. The results limit the direction of each star's motion to sectors less than 90° wide. None of the five stars are moving away from the Carina Nebula's central clusters as runaway stars would be, confirming that a candidate bow shock is not necessarily indicative of a runaway star. Two of the five stars are moving tangentially relative to the orientation of their candidate bow shocks, both of which point at the OB cluster Trumpler 14. In these cases, the large-scale flow of the interstellar medium, powered by feedback from the cluster, appears to dominate over the motion of the star in producing the observed candidate bow shock. The remaining three stars all have some component of motion towards the central clusters, meaning that we cannot distinguish whether their candidate bow shocks are indicators of stellar motion, of the flow of ambient gas or of density gradients in their surroundings. In addition, these stars' lack of outward motion hints that the distributed massive-star population in Carina's South Pillars region formed in place, rather than migrating out from the association's central clusters.

  11. On the observability of bow shocks of Galactic runaway OB stars

    NASA Astrophysics Data System (ADS)

    Meyer, D. M.-A.; van Marle, A.-J.; Kuiper, R.; Kley, W.

    2016-06-01

    Massive stars that have been ejected from their parent cluster and supersonically sailing away through the interstellar medium (ISM) are classified as exiled. They generate circumstellar bow-shock nebulae that can be observed. We present two-dimensional, axisymmetric hydrodynamical simulations of a representative sample of stellar wind bow shocks from Galactic OB stars in an ambient medium of densities ranging from nISM = 0.01 up to 10.0 cm- 3. Independently of their location in the Galaxy, we confirm that the infrared is the most appropriated waveband to search for bow shocks from massive stars. Their spectral energy distribution is the convenient tool to analyse them since their emission does not depend on the temporary effects which could affect unstable, thin-shelled bow shocks. Our numerical models of Galactic bow shocks generated by high-mass ( ≈ 40 M⊙) runaway stars yield H α fluxes which could be observed by facilities such as the SuperCOSMOS H-Alpha Survey. The brightest bow-shock nebulae are produced in the denser regions of the ISM. We predict that bow shocks in the field observed at H α by means of Rayleigh-sensitive facilities are formed around stars of initial mass larger than about 20 M⊙. Our models of bow shocks from OB stars have the emission maximum in the wavelength range 3 ≤ λ ≤ 50 μm which can be up to several orders of magnitude brighter than the runaway stars themselves, particularly for stars of initial mass larger than 20 M⊙.

  12. SCUBA and HIRES Results for Protostellar Cores in the MON OB1 Dark Cloud

    NASA Astrophysics Data System (ADS)

    Wolf-Chase, G.; Moriarty-Schieven, G.; Fich, M.; Barsony, M.

    1999-05-01

    We have used HIRES-processing of IRAS data and point-source modelling techniques (Hurt & Barsony 1996; O'Linger 1997; Barsony et al. 1998), together with submillimeter continuum imaging using the Submillimeter Common-User Bolometer Array (SCUBA) on the 15-meter James Clerk Maxwell Telescope (JCMT), to search CS cores in the Mon OB1 dark cloud (Wolf-Chase, Walker, & Lada 1995; Wolf-Chase & Walker 1995) for deeply embedded sources. These observations, as well as follow-up millimeter photometry at the National Radio Astronomy Observatory (NRAO) 12-meter telescope on Kitt Peak, have lead to the identification of two Class 0 protostellar candidates, which were previously unresolved from two brighter IRAS point sources (IRAS 06382+0939 & IRAS 06381+1039) in this cloud. Until now, only one Class 0 object had been confirmed in Mon OB1; the driving source of the highly-collimated outflow NGC 2264 G (Ward-Thompson, Eiroa, & Casali 1995; Margulis et al. 1990; Lada & Fich 1996), which lies well outside the extended CS cores. One of the new Class 0 candidates may be an intermediate-mass source associated with an H_2O maser, and the other object is a low-mass source which may be associated with a near-infrared jet, and possibly with a molecular outflow. We report accurate positions for the new Class 0 candidates, based on the SCUBA images, and present new SEDs for these sources, as well as for the brighter IRAS point sources. A portion of this work was performed while GWC held a President's Fellowship from the University of California. MB and GWC gratefully acknowledge financial support from MB's NSF CAREER Grant, AST97-9753229.

  13. Rewriting the Star-Formation History of the Nearest OB Association

    NASA Astrophysics Data System (ADS)

    Mamajek, Eric E.; Pecaut, Mark J.; Nguyen, Duy C.; Bubar, Eric J.

    2013-07-01

    The Sco-Cen (Sco OB2) OB association is the nearest region of recent massive star formation to the Sun. Sco-Cen is important for understanding the star-formation history of giant molecular cloud complexes, constraining circumstellar disk evolution, and providing samples of age-dated substellar objects and imaged planetary companions. Here we summarize some recent results on the classic Sco-Cen subgroups Upper Sco (US), Upper Cen-Lup (UCL), and Lower Cen-Cru (LCC): (1) isochronal analysis of the >1 Msun stars in the Upper Scorpius subgroup shows it to be twice as old as previously thought (˜11 Myr vs. ˜5 Myr), (2) analysis of high resolution optical spectra of FGK-type Sco-Cen members are consistent with the subgroups having solar metallicity, (3) we briefly describe a new subgroup dubbed "Lower Sco". Lastly, we find that the disk census of Sco-Cen members taken together are consistent with a protoplanetary disk fraction e-folding decay timescale of ˜4.5 Myr. This e-folding timescale is nearly twice that inferred previously for a large sample of nearby young stellar groups (~2.5 Myr ; see review of Mamajek 2009, AIPC, 1158, 3), but is consistent with the recent findings of Bell et al. (2013, MNRAS, in press; arxiv/1306.3237), which revised the ages of several benchmark clusters older by roughly a factor of two. The near doubling of the protoplanetary disk decay timescale may have important implications for inferring the lifetimes of early evolutionary stages of protostars, as well the efficiency of formation and subsequent migrational evolution of young gas giant planets.

  14. Hydrodynamic effects on microcapillary motility and chemotaxis assays of Methylosinus trichosporium OB3b.

    PubMed Central

    Shonnard, D R; Taylor, R T; Tompson, A; Knapp, R B

    1992-01-01

    A study of the random motility and chemotaxis of Methylosinus trichosporium OB3b was conducted by using Palleroni-chamber microcapillary assay procedures. Under the growth conditions employed, this methanotroph was observed qualitatively with a microscope to be either slightly motile or essentially nonmotile. However, the cells did not not respond in the microcapillary assays in the manner expected for nonmotile Brownian particles. As a consequence, several hydrodynamic effects on these Palleroni microcapillary assays were uncovered. In the random-motility microcapillary assay, nondiffusive cell accumulations occurred that were strongly dependent upon cell concentration. An apparent minimal random-motility coefficient (mu) for this bacterial cell of 1.0 x 10(-7) cm2/s was estimated from microcapillary assays. A simple alternative spectrophotometric assay, based upon gravitational settling, was developed and shown to be an improvement over the Palleroni microcapillary motility assay for M. trichosporium OB3b in that it yielded a more-accurate threefold-lower random-motility coefficient. In addition, it provided a calculation of the gravitational-settling velocity. In the chemotaxis microcapillary assay, the apparent chemotactic responses were strongest for the highest test-chemical concentrations in the microcapillaries, were correlated with microcapillary fluid density, and were strongly dependent upon the microcapillary volume. A simple method to establish the maximal concentration of a chemical that can be tested and to quantify any contributions of abiotic convection is described. Investigators should be aware of the potential problems due to density-driven convection when using these commonly employed microcapillary assays for studying cells which have low motilities. PMID:1444383

  15. Cerium Regulates Expression of Alternative Methanol Dehydrogenases in Methylosinus trichosporium OB3b

    PubMed Central

    Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan; Turpin, Erick A.; DiSpirito, Alan A.

    2015-01-01

    Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a “copper switch.” At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availability of copper. In many methanotrophs there are also multiple methanol dehydrogenases (MeDHs), one based on Mxa and another based on Xox. Mxa-MeDH is known to have calcium in its active site, while Xox-MeDHs have been shown to have rare earth elements in their active site. We show here that the expression levels of Mxa-MeDH and Xox-MeDH in Methylosinus trichosporium OB3b significantly decreased and increased, respectively, when grown in the presence of cerium but the absence of copper compared to the absence of both metals. Expression of sMMO and pMMO was not affected. In the presence of copper, the effect of cerium on gene expression was less significant, i.e., expression of Mxa-MeDH in the presence of copper and cerium was slightly lower than in the presence of copper alone, but Xox-MeDH was again found to increase significantly. As expected, the addition of copper caused sMMO and pMMO expression levels to significantly decrease and increase, respectively, but the simultaneous addition of cerium had no discernible effect on MMO expression. As a result, it appears Mxa-MeDH can be uncoupled from methane oxidation by sMMO in M. trichosporium OB3b but not from pMMO. PMID:26296730

  16. Colliding filaments and a massive dense core in the Cygnus OB 7 molecular cloud

    SciTech Connect

    Dobashi, Kazuhito; Shimoikura, Tomomi; Akisato, Ko; Ohashi, Kenjiro; Nakagomi, Keisuke; Matsumoto, Tomoaki; Saito, Hiro

    2014-12-10

    We report the results of molecular line observations carried out toward a massive dense core in the Cyg OB 7 molecular cloud. The core has an extraordinarily large mass (∼1.1 × 10{sup 4} M {sub ☉}) and size (∼2 × 5 pc{sup 2}), but there is no massive young star forming therein. We observed this core in various molecular lines such as C{sup 18}O(J = 1-0) using the 45 m telescope at Nobeyama Radio Observatory. We find that the core has an elongated morphology consisting of several filaments and core-like structures. The filaments are massive (10{sup 2}-10{sup 3} M {sub ☉}), and they are apparently colliding with one another. Some candidates for young stellar objects are distributed around their intersection, suggesting that the collisions of the filaments may have influenced their formation. To understand the formation and evolution of such colliding filaments, we performed numerical simulations using the adaptive mesh refinement technique, adopting the observed core parameters (the mass and size) as the initial conditions. The results indicate that the filaments are formed as seen in other earlier simulations for small cores in the literature, but we could not reproduce the collisions of the filaments simply by assuming a large initial mass and size. We find that collisions of the filaments occur only when there is a large velocity gradient in the initial core, in a sense compressing it. We suggest that the observed core was actually compressed by an external effect, e.g., shocks from nearby supernova remnants, including HB 21 which has been suggested to be interacting with the Cyg OB 7 molecular cloud.

  17. γ-ray production in young open clusters: Berk 87, Cyg OB2 and Westerlund 2

    NASA Astrophysics Data System (ADS)

    Bednarek, W.

    2007-11-01

    Young open clusters are sites of cosmic ray acceleration as indicated by recent detections of the TeV γ-ray sources in the directions of two open clusters (Cyg OB2 and Westerlund 2). In fact, up to now a few different scenarios for acceleration of particles inside open clusters have been considered, i.e. shocks in massive star winds, pulsars and their nebulae, supernova shocks, massive compact binaries. Here we consider in detail the radiation processes due to both electrons and hadrons accelerated inside the open cluster. As a specific scenario, we apply the acceleration process at the shocks arising in the winds of Wolf-Rayet (WR) type stars. Particles diffuse through the medium of the open cluster during the activity time of the acceleration scenario defined by the age of the WR star. They interact with the matter and radiation, at first inside the open cluster and, later in the dense surrounding clouds. We calculate the broad-band spectrum in different processes for three exemplary open clusters (Berk 87, Cyg OB2, Westerlund 2) for which the best observational constraints on the spectra are at present available. It is assumed that the high-energy phenomena, observed from the X-ray up to the GeV-TeV γ-ray energies, are related to each other. We conclude that the most likely description of the radiation processes in these objects is achieved in the hybrid (leptonic-hadronic) model in which leptons are responsible for the observed X-ray and GeV γ-ray emission and hadrons are responsible for the TeV γ-ray emission.

  18. Distribution of global fallouts cesium-137 in taiga and tundra catenae at the Ob River basin

    NASA Astrophysics Data System (ADS)

    Semenkov, I. N.; Usacheva, A. A.; Miroshnikov, A. Yu.

    2015-03-01

    The classification of soil catenae at the Ob River basin is developed and applied. This classification reflects the diverse geochemical conditions that led to the formation of certain soil bodies, their combinations and the migration fields of chemical elements. The soil and geochemical diversity of the Ob River basin catenae was analyzed. The vertical and lateral distribution of global fallouts cesium-137 was studied using the example of the four most common catenae types in Western Siberia tundra and taiga. In landscapes of dwarf birches and dark coniferous forests on gleysols, cryosols, podzols, and cryic-stagnosols, the highest 137Cs activity density and specific activity are characteristic of the upper soil layer of over 30% ash, while the moss-grass-shrub cover is characterized by low 137Cs activity density and specific activity. In landscapes of dwarf birches and pine woods on podzols, the maximum specific activity of cesium-137 is typical for moss-grass-shrub cover, while the maximum reserves are concentrated in the upper soil layer of over 30% ash. Bog landscapes and moss-grass-shrub cover are characterized by a minimum activity of 137Cs, and its reserves in soil generally decrease exponentially with depth. The cesium-137 penetration depth increases in oligotrophic histosols from northern to middle taiga landscapes from 10-15 to 40 cm. 137Cs is accumulated in oligotrophic histosols for increases in pH from 3.3 to 4.0 and in concretionary interlayers of pisoplinthic-cryic-histic-stagnosols. Cryogenic movement, on the one hand, leads to burying organic layers enriched in 137Cs and, on the other hand, to deducing specific activity when mixed with low-active material from lower soil layers.

  19. Cerium regulates expression of alternative methanol dehydrogenases in Methylosinus trichosporium OB3b.

    PubMed

    Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan; Turpin, Erick A; DiSpirito, Alan A; Semrau, Jeremy D

    2015-11-01

    Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a "copper switch." At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availability of copper. In many methanotrophs there are also multiple methanol dehydrogenases (MeDHs), one based on Mxa and another based on Xox. Mxa-MeDH is known to have calcium in its active site, while Xox-MeDHs have been shown to have rare earth elements in their active site. We show here that the expression levels of Mxa-MeDH and Xox-MeDH in Methylosinus trichosporium OB3b significantly decreased and increased, respectively, when grown in the presence of cerium but the absence of copper compared to the absence of both metals. Expression of sMMO and pMMO was not affected. In the presence of copper, the effect of cerium on gene expression was less significant, i.e., expression of Mxa-MeDH in the presence of copper and cerium was slightly lower than in the presence of copper alone, but Xox-MeDH was again found to increase significantly. As expected, the addition of copper caused sMMO and pMMO expression levels to significantly decrease and increase, respectively, but the simultaneous addition of cerium had no discernible effect on MMO expression. As a result, it appears Mxa-MeDH can be uncoupled from methane oxidation by sMMO in M. trichosporium OB3b but not from pMMO.

  20. Stagnant Shells in the Vicinity of the Dusty Wolf-Rayet-OB Binary WR 112

    NASA Astrophysics Data System (ADS)

    Lau, R. M.; Hankins, M. J.; Schödel, R.; Sanchez-Bermudez, J.; Moffat, A. F. J.; Ressler, M. E.

    2017-02-01

    We present high spatial resolution mid-infrared images of the nebula around the late-type carbon-rich Wolf-Rayet (WC)-OB binary system WR 112 taken by the recently upgraded VLT spectrometer and imager for the mid-infrared (VISIR) with the PAH1, Ne ii_2, and Q3 filters. The observations reveal a morphology resembling a series of arc-like filaments and broken shells. Dust temperatures and masses are derived for each of the identified filamentary structures, which exhibit temperatures ranging from {179}-6+8 K at the exterior W2 filament to {355}-25+37 K in the central 3″. The total dust mass summed over the features is 2.6 ± 0.4 × 10-5 M⊙. A multi-epoch analysis of mid-IR photometry of WR 112 over the past ˜20 years reveals no significant variability in the observed dust temperature and mass. The morphology of the mid-IR dust emission from WR 112 also exhibits no significant expansion from imaging data taken in 2001, 2007, and 2016, which disputes the current interpretation of the nebula as a high expansion velocity (˜1200 km s-1) “pinwheel”-shaped outflow driven by the central WC-OB colliding-wind binary. An upper limit of ≲120 km s-1 is derived for the expansion velocity assuming a distance of 4.15 kpc. The upper limit on the average total mass-loss rate from the central 3″ of WR 112 is estimated to be ≲8 × 10-6 M⊙ year-1. We leave its true nature as an open question, but propose that the WR 112 nebula may have formed in the outflow during a previous red or yellow supergiant phase of the central Wolf-Rayet star.

  1. Metabolic profiling of ob/ob mouse fatty liver using HR-MAS (1)H-NMR combined with gene expression analysis reveals alterations in betaine metabolism and the transsulfuration pathway.

    PubMed

    Gogiashvili, Mikheil; Edlund, Karolina; Gianmoena, Kathrin; Marchan, Rosemarie; Brik, Alexander; Andersson, Jan T; Lambert, Jörg; Madjar, Katrin; Hellwig, Birte; Rahnenführer, Jörg; Hengstler, Jan G; Hergenröder, Roland; Cadenas, Cristina

    2017-02-01

    Metabolic perturbations resulting from excessive hepatic fat accumulation are poorly understood. Thus, in this study, leptin-deficient ob/ob mice, a mouse model of fatty liver disease, were used to investigate metabolic alterations in more detail. Metabolites were quantified in intact liver tissues of ob/ob (n = 8) and control (n = 8) mice using high-resolution magic angle spinning (HR-MAS) (1)H-NMR. In addition, after demonstrating that HR-MAS (1)H-NMR does not affect RNA integrity, transcriptional changes were measured by quantitative real-time PCR on RNA extracted from the same specimens after HR-MAS (1)H-NMR measurements. Importantly, the gene expression changes obtained agreed with those observed by Affymetrix microarray analysis performed on RNA isolated directly from fresh-frozen tissue. In total, 40 metabolites could be assigned in the spectra and subsequently quantified. Quantification of lactate was also possible after applying a lactate-editing pulse sequence that suppresses the lipid signal, which superimposes the lactate methyl resonance at 1.3 ppm. Significant differences were detected for creatinine, glutamate, glycine, glycolate, trimethylamine-N-oxide, dimethylglycine, ADP, AMP, betaine, phenylalanine, and uridine. Furthermore, alterations in one-carbon metabolism, supported by both metabolic and transcriptional changes, were observed. These included reduced demethylation of betaine to dimethylglycine and the reduced expression of genes coding for transsulfuration pathway enzymes, which appears to preserve methionine levels, but may limit glutathione synthesis. Overall, the combined approach is advantageous as it identifies changes not only at the single gene or metabolite level but also deregulated pathways, thus providing critical insight into changes accompanying fatty liver disease. Graphical abstract A Evaluation of RNA integrity before and after HR-MAS (1)H-NMR of intact mouse liver tissue. B Metabolite concentrations and gene

  2. Controlling the onset of OB/OM in a semiconductor quantum well system in an inverted Y-type configuration

    NASA Astrophysics Data System (ADS)

    Raheli, Ali; Hamedi, H. R.; Sahrai, M.

    2016-01-01

    The problem of optical bistability (OB) and optical multistability (OM) is numerically investigated in a four-level inverted Y-type semiconductor quantum well (SQW) structure immersed in a unidirectional ring cavity. In the four-level SQW system under consideration, a closed loop configuration is coupled to the upper level through a tunable probe field. We show that the OB threshold intensity can be controlled via the intensity of coupling fields which gives rise to the absorption variation of the probe field. In addition, due to the existence of the closed-loop configuration, the OB and OM behaviors of the proposed SQW medium are dependent on the relative phase of the applied fields. It is found that the OB can be switched to OM or vice versa by properly adjusting the relative phase of the applied fields. The results may provide new possibilities in real experiments for realizing an all-optical switching or coding element in a solid-state platform.

  3. The gas-dust complex NGC 7822 + S 171 (W1) linked to the CEP OB4 association

    NASA Astrophysics Data System (ADS)

    Lozinskaya, T. A.; Sitnik, T. G.; Toropova, M. S.

    1987-10-01

    The results of investigations of the gas-and-dust complex connected with the young association Cep OB4 and the cluster Be 59 are presented. Monochromatic photographs of the bright emission nebulae S 171 (W1) and NGC 7822 are obtained. The expansion velocity of a faint extended shell around W1 and Cep OB4 is confirmed to be equal to 30 - 40 km s-1. IR observations are presented. A second "inner" shell is revealed. The numerous molecular and dust clouds, emission nebulae and two faint extended shells in this region lead to the conclusion that they form a single complex associated with Cep OB4 and the young cluster Be 59. The shell-type morphology of the complex seems to be created by the stellar wind of Cep OB4 and Be 59. The expansion velocity could be explained by a supernova explosion about 3×105years ago or by a short-lived (or invisible) source of a strong stellar wind.

  4. Methanol suppression of trichloroethylene degradation by Methylosinus trichosporium (OB3b) and methane-oxidizing mixed cultures

    SciTech Connect

    Eng, W.; Palumbo, A.V.; Strandberg, G.W.; Sriharan, S.

    1991-12-31

    The effect of methanol on trichloroethylene (TCE) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. Methanol was found to relieve growth inhibition of Methylosinus trichosporium (OB3b) at high (14 mg/L) TCE concentrations. Degradation of TCE was determined by both radiolabeling and gas chromatography techniques. When cultures were grown on methanol over 10 to 14 d with 0.3 mg/L TCE, OB3b degraded 16.89 {+-} 0.82% (mean {+-} SD) of the TCE, and a mixed culture (DT type II) degraded 4.55 {+-} 0.11%. Mixed culture (JS type I) degraded 4.34 {+-} 0.06% of the TCE. When grown on methane with 0.3 mg/L TCE, 32.93 {+-} 2.01% of the TCE was degraded by OB3b, whereas the JS culture degraded 24.3 {+-} 1.38% of the TCE, and the DT culture degraded 34.3 {+-} 2.97% of the TCE. The addition of methanol to cultures grown on methane reduced TCE degradation to 16.21 {+-} 1.17% for OB3b and to 5.08 {+-} 0.56% for JS. Although methanol reduces the toxicity of TCE to the cultures, biodegradation of TCE cannot be sustained in methanol-grown cultures. Since high TCE concentrations appear to inhibit methane uptake and growth, we suggest the primary toxicity of TCE is directed towards the methane monooxygenase.

  5. Lithospheric structure beneath the extinct ridge of South China Sea: Constraints from Rayleigh wave phase velocity tomography using OBS data

    NASA Astrophysics Data System (ADS)

    Yang, T.; Le, B. M.; passive-Source Seismic Team, S.

    2016-12-01

    What would happen when a mid-ocean-ridge stops spreading? Global occurrences of such ridges appear to indicate that magmatic activities had continued for million years after ridges were abandoned and often formed seamount chains over ridges. The extinct ridge and the seamount chain at the South China Sea represent one classic example of such ridges. To understand this unique process and the lithospheric and deep mantle structure, we carry out a Rayleigh wave phase velocity tomography using data from a passive-source OBS array experiment in South China Sea from 2012 to 2013. We correct OBS clock errors by using Scholte waves retrieved through cross-correlating hydrophone records of each OBS pair. 60 regional and teleseismic events with high quality Rayleigh waves are selected and their dispersion curves at the OBS array are used to inverse the phase velocities of periods from 15 s to 100 s. The shear wave velocity model derived from phase velocities of all periods shows a strong low-velocity zone situated beneath the seamounts starting at about 30 km depth. The lithosphere thickness of the extinct ridge inferred from this model provide insights on the cooling process and magmatism at this unique oceanic setting. In addition, our model images the tear of the subducting South China Sea plate beneath the Manila trench and Luzon island, which is clearly generated by the subduction of the extinct ridge and overriding seamounts.

  6. Crystal Structures of RMI1 and RMI2, Two OB-Fold Regulatory Subunits of the BLM Complex

    SciTech Connect

    Wang, Feng; Yang, Yuting; Singh, Thiyam Ramsing; Busygina, Valeria; Guo, Rong; Wan, Ke; Wang, Weidong; Sung, Patrick; Meetei, Amom Ruhikanta; Lei, Ming

    2010-11-05

    Mutations in BLM, a RecQ-like helicase, are linked to the autosomal recessive cancer-prone disorder Bloom's syndrome. BLM associates with topoisomerase (Topo) III{alpha}, RMI1, and RMI2 to form the BLM complex that is essential for genome stability. The RMI1-RMI2 heterodimer stimulates the dissolution of double Holliday junction into non-crossover recombinants mediated by BLM-Topo III{alpha} and is essential for stabilizing the BLM complex. However, the molecular basis of these functions of RMI1 and RMI2 remains unclear. Here we report the crystal structures of multiple domains of RMI1-RMI2, providing direct confirmation of the existence of three oligonucleotide/oligosaccharide binding (OB)-folds in RMI1-RMI2. Our structural and biochemical analyses revealed an unexpected insertion motif in RMI1N-OB, which is important for stimulating the dHJ dissolution. We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance.

  7. Work and Family Research in IO/OB: Content Analysis and Review of the Literature (1980-2002)

    ERIC Educational Resources Information Center

    Eby, Lillian T.; Casper, Wendy J.; Lockwood, Angie; Bordeaux, Chris; Brinley, Andi

    2005-01-01

    This monograph reviews 190 work-family studies published in IO/OB journals from 1980 to 2002. The results of a content analysis are presented which catalog these articles with respect to the study focus, nature and direction of the proposed effects, and predictor, criterion, and mediator variables examined. Then a narrative review of the articles…

  8. Work and Family Research in IO/OB: Content Analysis and Review of the Literature (1980-2002)

    ERIC Educational Resources Information Center

    Eby, Lillian T.; Casper, Wendy J.; Lockwood, Angie; Bordeaux, Chris; Brinley, Andi

    2005-01-01

    This monograph reviews 190 work-family studies published in IO/OB journals from 1980 to 2002. The results of a content analysis are presented which catalog these articles with respect to the study focus, nature and direction of the proposed effects, and predictor, criterion, and mediator variables examined. Then a narrative review of the articles…

  9. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b

    SciTech Connect

    Gu, Wenyu; Farhan Ul Haque, Muhammad; DiSpirito, Alan A.; Semrau, Jeremy D.

    2016-05-12

    It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.

  10. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b

    SciTech Connect

    Gu, Wenyu; Farhan Ul Haque, Muhammad; DiSpirito, Alan A.; Semrau, Jeremy D.

    2016-05-12

    It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.

  11. The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs.

    PubMed

    Choi, Kyung H; Lakamp-Hawley, Amanda S; Kolar, Carol; Yan, Ying; Borgstahl, Gloria E O; Ouellette, Michel M

    2015-08-01

    The POT1 protein plays a critical role in telomere protection and telomerase regulation. POT1 binds single-stranded 5'-TTAGGGTTAG-3' and forms a dimer with the TPP1 protein. The dimer is recruited to telomeres, either directly or as part of the Shelterin complex. Human POT1 contains two Oligonucleotide/Oligosaccharide Binding (OB) fold domains, OB1 and OB2, which make physical contact with the DNA. OB1 recognizes 5'-TTAGGG whereas OB2 binds to the downstream TTAG-3'. Studies of POT1 proteins from other species have shown that some of these proteins are able to recognize a broader variety of DNA ligands than expected. To explore this possibility in humans, we have used SELEX to reexamine the sequence-specificity of the protein. Using human POT1 as a selection matrix, high-affinity DNA ligands were selected from a pool of randomized single-stranded oligonucleotides. After six successive rounds of selection, two classes of high-affinity targets were obtained. The first class was composed of oligonucleotides containing a cognate POT1 binding sites (5'-TTAGGGTTAG-3'). The second and more abundant class was made of molecules that carried a novel non-telomeric consensus: 5'-TNCANNAGKKKTTAGG-3' (where K = G/T and N = any base). Binding studies showed that these non-telomeric sites were made of an OB1-binding motif (TTAGG) and a non-telomeric motif (NT motif), with the two motifs recognized by distinct regions of the OB1 domain. POT1 interacted with these non-telomeric binding sites with high affinity and specificity, even when bound to its dimerization partner TPP1. This intrinsic ability of POT1 to recognize NT motifs raises the possibility that the protein may fulfill additional functions at certain non-telomeric locations of the genome, in perhaps gene transcription, replication, or repair. Copyright © 2015 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  12. Waveform Inversion of OBS Data and Illumination/Resolution Analyses on Marine Seismic Data Acquisitions by the Adjoint Wavefield Method

    NASA Astrophysics Data System (ADS)

    Chen, H.; Li, K.

    2012-12-01

    We applied a wave-equation based adjoint wavefield method for seismic illumination/resolution analyses and full waveform inversion. A two-way wave-equation is used to calculate directional and diffracted energy fluxes for waves propagating between sources and receivers to the subsurface target. The first-order staggered-grid pressure-velocity formulation, which lacks the characteristic of being self-adjoint is further validated and corrected to render the modeling operator before its practical application. Despite most published papers on synthetic kernel research, realistic applications to two field experiments are demonstrated and emphasize its practical needs. The Fréchet sensitivity kernels are used to quantify the target illumination conditions. For realistic illumination measurements and resolution analyses, two completely different survey geometries and nontrivial pre-conditioning strategies based on seismic data type are demonstrated and compared. From illumination studies, particle velocity responses are more sensitive to lateral velocity variations than pressure records. For waveform inversion, the more accurately estimated velocity model obtained the deeper the depth of investigation would be reached. To achieve better resolution and illumination, closely spaced OBS receiver interval is preferred. Based on the results, waveform inversion is applied for a gas hydrate site in Taiwan for shallow structure and BSR detection. Full waveform approach potentially provides better depth resolution than ray approach. The quantitative analyses, a by-product of full waveform inversion, are useful for quantifying seismic processing and depth migration strategies.llumination/resolution analysis for a 3D MCS/OBS survey in 2008. Analysis of OBS data shows that pressure (top), horizontal (middle) and vertical (bottom) velocity records produce different resolving power for gas hydrate exploration. ull waveform inversion of 8 OBS data along Yuan-An Ridge in SW Taiwan

  13. Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.

    PubMed

    Wang, Li Kai; Nair, Pravin A; Shuman, Stewart

    2008-08-22

    NAD(+)-dependent DNA ligases (LigAs) are ubiquitous in bacteria and essential for growth. LigA enzymes have a modular structure in which a central catalytic core composed of nucleotidyltransferase and oligonucleotide-binding (OB) domains is linked via a tetracysteine zinc finger to distal helix-hairpin-helix (HhH) and BRCT (BRCA1-like C-terminal) domains. The OB and HhH domains contribute prominently to the protein clamp formed by LigA around nicked duplex DNA. Here we conducted a structure-function analysis of the OB and HhH domains of Escherichia coli LigA by alanine scanning and conservative substitutions, entailing 43 mutations at 22 amino acids. We thereby identified essential functional groups in the OB domain that engage the DNA phosphodiester backbone flanking the nick (Arg(333)); penetrate the minor grove and distort the nick (Val(383) and Ile(384)); or stabilize the OB fold (Arg(379)). The essential constituents of the HhH domain include: four glycines (Gly(455), Gly(489), Gly(521), Gly(553)), which bind the phosphate backbone across the minor groove at the outer margins of the LigA-DNA interface; Arg(487), which penetrates the minor groove at the outer margin on the 3 (R)-OH side of the nick; and Arg(446), which promotes protein clamp formation via contacts to the nucleotidyltransferase domain. We find that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation.

  14. Methanobactin from Methylocystis sp. strain SB2 affects gene expression and methane monooxygenase activity in Methylosinus trichosporium OB3b.

    PubMed

    Farhan Ul-Haque, Muhammad; Kalidass, Bhagyalakshmi; Vorobev, Alexey; Baral, Bipin S; DiSpirito, Alan A; Semrau, Jeremy D

    2015-04-01

    Methanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. When Methylosinus trichosporium OB3b was grown in the presence of 1 μM CuCl2, expression of mmoX, encoding a subunit of the hydroxylase component of sMMO, was very low. mmoX expression increased, however, when methanobactin from Methylocystis sp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated with M. trichosporium OB3b. If M. trichosporium OB3b was grown in the absence of CuCl2, the mmoX expression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure of Methylosinus trichosporium OB3b to SB2-Mb had no effect on expression of mbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2. mbnA expression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin "piracy" may be commonplace.

  15. Temperature, gravity, and bolometric correction scales for non-supergiant OB stars

    NASA Astrophysics Data System (ADS)

    Nieva, M.-F.

    2013-02-01

    Context. Precise and accurate determinations of the atmospheric parameters effective temperature and surface gravity are mandatory to derive reliable chemical abundances in OB stars. Furthermore, fundamental parameters like distances, masses, radii, luminosities can also be derived from the temperature and gravity of the stars. Aims: Atmospheric parameters recently determined at high precision with several independent spectroscopic indicators in non-local thermodynamic equilibrium, with typical uncertainties of ~300 K for temperature and of ~0.05 dex for gravity, are employed to calibrate photometric relationships. This is in order to investigate whether a faster tool to estimate atmospheric parameters can be provided. Methods: Temperatures and gravities of 30 calibrators, i.e. well-studied OB main sequence to giant stars in the solar neighbourhood, are compared to reddening-independent quantities of the Johnson and Strömgren photometric systems, assuming normal reddening. In addition, we examine the spectral and luminosity classification of the star sample and compute bolometric corrections. Results: Calibrations of temperatures and gravities are proposed for various photometric indices and spectral types. Once the luminosity of the stars is well known, effective temperatures can be determined at a precision of ~400 K for luminosity classes III/IV and ~800 K for luminosity class V. Furthermore, surface gravities can reach internal uncertainties as low as ~0.08 dex when using our calibration to the Johnson Q-parameter. Similar precision is achieved for gravities derived from the β-index and the precision is lower for both atmospheric parameters when using the Strömgren indices [c1] and [u - b] . In contrast, external uncertainties are larger for the Johnson than for the Strömgren calibrations. Our uncertainties are smaller than typical differences among other methods in the literature, reaching values up to ± 2000 K for temperature and ± 0.25 dex for gravity

  16. The 2.35 year itch of Cygnus OB2 #9. II. Radio monitoring

    NASA Astrophysics Data System (ADS)

    Blomme, R.; Nazé, Y.; Volpi, D.; De Becker, M.; Prinja, R. K.; Pittard, J. M.; Parkin, E. R.; Absil, O.

    2013-02-01

    Context. Cyg OB2 #9 is one of a small set of non-thermal radio emitting massive O-star binaries. The non-thermal radiation is due to synchrotron emission in the colliding-wind region. Cyg OB2 #9 has only recently been discovered to be a binary system, and a multi-wavelength campaign was organized to study its 2011 periastron passage. Aims: We want to better determine the parameters of this system and model the wind-wind collision. This will lead to a better understanding of the Fermi mechanism that accelerates electrons up to relativistic speeds in shocks and its occurrence in colliding-wind binaries. We report here on the results of the radio observations obtained in the monitoring campaign and present a simple model to interpret the data. Methods: We used the Expanded Very Large Array (EVLA) radio interferometer to obtain 6 cm and 20 cm continuum fluxes during the Cyg OB2 #9 periastron passage in 2011. We introduce a simple model to solve the radiative transfer in the stellar winds and the colliding-wind region, and thus determine the expected behaviour of the radio light curve. Results: The observed radio light curve shows a steep drop in flux sometime before periastron. The fluxes drop to a level that is comparable to the expected free-free emission from the stellar winds, suggesting that the non-thermal emitting region is completely hidden at that time. After periastron passage, the fluxes slowly increase. We use the asymmetry of the light curve to show that the primary has the stronger wind. This is somewhat unexpected if we use the astrophysical parameters based on theoretical calibrations. But it becomes entirely feasible if we take into account that a given spectral type-luminosity class combination covers a range of astrophysical parameters. The colliding-wind region also contributes to the free-free emission, which can help explain the high values of the spectral index seen after periastron passage. Combining our data with older Very Large Array (VLA

  17. Fault Characterization in the Sea of Marmara (Turkey) Using OBS and Land Seismic Stations

    NASA Astrophysics Data System (ADS)

    Pinar, Ali; Yamamoto, Yojiro; Comoglu, Mustafa; Polat, Remzi; Turhan, Fatih; Takahashi, Narumi; Kalafat, Dogan; Citak, Seckin

    2016-04-01

    The fault segments of the North Anatolian fault (NAF) occurring between Tekirdag basin and Kumburgaz basin are investigated using 15 Ocean Bottom Seismic (OBS) stations. The OBS stations were deployed closely around the fault trace of NAF. During the observation period from September, 2014 until July, 2015 more than one thousand microearthquakes were determined. No uniform seismicity pattern was observed along strike and along dip of the fault segments in an area spanning 100 km from East to West of Marmara Sea. The western fault segments exhibit relatively higher and deeper seismic activity while the eastern segment show shallower and relatively lower seismic activity. Integrating the first motion polarity data from the land based stations of Kandilli Observatory and Earthquake Research Institute (KOERI) with the polarity data acquired from the OBS stations the focal mechanisms of 173 micro-earthquakes were determined. Most of the fault plane solutions indicate predominantly strike-slip mechanism. Several clusters of events are identified along the E-W extending NAF. We derive a focal mechanism for the individual events whenever the number of the polarities are sufficient. In addition, simultaneous inversion of the polarities in a cluster are done to retrieve a stress tensor along with focal mechanisms of the individual events in a cluster. A unique cluster of focal mechanisms was obtained from the events taking place in Western High (WH) region located between Tekirdag Basin (TB) and Central Basin (CB). Several features of this cluster are noticeable; 1) the site is the most seismically active part in Marmara Sea, 2) the site is the locus of the deepest events in the Sea of Marmara, 3) the shallower part of this segment is seismically less active, 4) two subgroups of P-axes of focal mechanisms exist; one oriented NW-SE and other oriented in N-S direction despite the proximity of the location of the events giving clues on the faulting dynamics. The N-S oriented P

  18. Focal mechanism and stress inversion in the Mendocino Triple Junction from OBS data

    NASA Astrophysics Data System (ADS)

    Li, D.; McGuire, J. J.; Liu, Y.

    2016-12-01

    The Mendocino Triple Junction (MTJ) is at the south end of the Cascadia subduction zone, where the North American, Gorda and Pacific Plates intersect. This region contains many active faults and experiences strong internal deformation, resulting in many small-to-intermediate size earthquakes. In 1992, A Mw7.2 thrust earthquake occurred on a shallow dipping fault parallel to the subduction interface, suggesting a relatively weak thrust fault that ruptures under the inferred trench-parallel compressive stress. This contrasts to the high strength of the subducting Gorda plate [Choy and McGarr, 2002] where most of the seismicity occurs. A Mw 5.7 rupture of the Mendocino Transform Fault that occurred just offshore on January 28, 2015 (See Gong and McGuirethis section). However, we did not identify any thrust mechanism aftershock during the following two weeks, using data acquired during the Cascadia Initiative ocean-bottom seismometers (OBS) Expedition 2012-2013, 2014-2015 as well as from the PBO and NCSN onshore networks, We obtain focal mechanisms for earthquakes near the MTJ with a cutoff magnitude of Ml 2.0. The OBS data improve both the number and resolution of the focal mechanisms compared to the onshore only catalogs. The associated azimuth distribution of selected earthquakes is suitable for resolving the focal mechanisms with the first P-arrival polarity [Hardebeck and Shear, 2002]. We also invert the principle stress orientations with the NCSN focal mechanisms from 1977 to 2016, using the method introduced by Hardebeck and Micheal, [2006]. To distinguish the spatial variation, we divide the earthquakes into three groups in depth, overlying continent, oceanic crust and oceanic mantle. We further divide each group into four subareas, based on the distance to Mendocino transform fault. Our preliminary results show that there are both vertical and lateral rotations of the principle compressive stress axes. Similar to previous studies, the orientation of

  19. Ambient noise cross-correlations applied to reservoir scale OBS-recordings

    NASA Astrophysics Data System (ADS)

    Weemstra, C.; Goertz, A.; Artman, B.; Boschi, L.

    2010-12-01

    Surface waves extracted from the ambient seismic wavefield via interferometry can be used for velocity inversion. In order to invert shear wave velocities at the reservoir scale, Bussat & Kugler (2009) adapted this approach to Scholte waves at frequencies up to 1 Hz. These waves were extracted from comparatively short OBS recordings, ranging from several hours to a few days. We have extended the ambient noise surface-wave analysis of this data set to the three seismic components. This passive seismic data set was acquired in April/May 2007. It is recorded over a ˜220 km2 survey area at an average depth of 360 m, offshore Norway. Data was recorded at 117 seabed locations by 16 ocean-bottom seismometers (OBS), equipped with a broadband seismometer and a differential pressure gauge (DPG). The instruments have a flat response to particle velocity between 240 s and 50 Hz and data was acquired with a sampling rate of 125 Hz. The stations were systematically relocated after 1 to 2 days of recording except for two stations which were recording continuously. The main energy in the data below 5 Hz stems from swell noise, ocean microseisms and Scholte waves traveling along the seabed. After filtering the multicomponent data between 0.01 and 10.0 Hz, we analyze them with respect to azimuthal variation of the incident wavefield in order to find directions of possible dominant sources that may violate the assumption of a diffuse wavefield and impact the retrieval of interstation Green’s functions. Different temporal normalization techniques are compared with respect to their effect on the Green’s function gathers. The ultimate goal of our effort is to understand the effect of temporal normalization on the decay of amplitude with distance, i.e. the relation between temporal normalization and attenuation of the obtained Green’s functions. Synthetic data are used to test this effect. We seek processing techniques that preserve the attenuation characteristics of the

  20. Constraining formation of the Eggvin Bank (West of Jan Mayen, N. Atlantic) from OBS data

    NASA Astrophysics Data System (ADS)

    Tan, P.; Breivik, A. J.; Mjelde, R.; Azuma, R.

    2015-12-01

    The anomalously high magma flux in the Eggvin Bank area has triggered new research efforts to better understand the crustal development in this area. The Eggvin Bank is located between the Jan Mayen Island and the west coast of Greenland. Some proposed origins of the Eggvin Bank are: a distinct plume located beneath Jan Mayen; an extension of the Iceland plume; minor spreading or leakage along West Jan Mayen Fracture Zone (WJMFZ); intruded continental crust extending from Jan Mayen Microcontinent (JMMC); and rifted Greenland sub-continental lithospheric mantle. In this first modern refraction seismic study of the Eggvin Bank, we present a 2D velocity model based on OBS data. The OBSs were deployed approx. N-S over the Eggvin Bank with good data quality constrained by 4 OBSs. The air-gun array used during OBS shooting produced good quality reflection data. Three distinct seamounts are observed along the profile: the northern seamount (water depth 730m), has a flat top with a thin sedimentary veneer on top, which indicates it has been eroded at sea surface; while the southern two seamounts, one (water depth 550m) is less flat with around 100m thick sedimentary units on top, another one is rounded with tiny sedimentary veneer on top having the shallowest water depth (460m). This could suggest that the southern seamounts are younger, since they are shallower but without obvious signs that they were subaerially exposed. However, increased cooling of the lithosphere across the WJMFZ in the north may also contribute to depth differences. A normal fault offsetting sedimentary strata (~300 m) in the Greenland Basin indicates recent tectonic activity north of the Eggvin Bank. The velocity modeling shows crustal thickness with large variations, ranging from 8 km to 14 km, where crustal thickness changes of 4-5 km are associated with 20-30 km wide segments with thick crust under the seamounts. The crust consists of three oceanic crustal layers: upper crust (2.8km/s-4.8km

  1. Exploring the Full Stellar Population of the Upper Scorpius OB Association

    NASA Astrophysics Data System (ADS)

    Preibisch, Thomas; Brown, Anthony G. A.; Bridges, Terry; Guenther, Eike; Zinnecker, Hans

    2002-07-01

    We investigate the stellar population and star formation history of the Upper Scorpius OB association, the most nearby region of recent massive star formation, over the full stellar mass range from 0.1 to 20 Msolar. The first part of this paper describes an extension of our large spectroscopic survey (Preibisch et al., published in 2001) for low-mass pre-main-sequence (PMS) stars in Upper Scorpius. Using the multiobject spectrograph 2dF at the Anglo-Australian Telescope, we obtained spectra of 469 stars with magnitudes R=12.5-18.0 in a 6 deg2 area. Among these, we find 68 new PMS stars, nearly all of them M-type stars, by their strong lithium absorption lines. The total area covered by our 2dF survey is now 9 deg2 and contains 166 new PMS stars. Combining these results with our earlier investigation (Preibisch & Zinnecker) yields a sample of 250 PMS stars in the mass range ~0.1 to ~2 Msolar. The location of these stars in the HR diagram suggests a mean age of 5 Myr without a significant age spread. In the second part of this paper, we also consider the population of 114 high-mass members identified in detailed Hipparcos studies. We construct a combined HR diagram for the 364 high- and low-mass members and find that the whole stellar population is very well characterized by a very narrow age distribution around 5 Myr. We estimate individual masses for all members and construct an empirical mass function covering the mass range from 0.1 up to 20 Msolar. A power-law fit to the mass function gives a slope of α~-2.6 above ~2 Msolar and a much flatter slope (α~-0.9) below ~0.6 Msolar. The initial mass function of Upper Sco is not identical, but within the errors consistent with recent determinations of the field initial mass function. There is certainly no deficit of low-mass stars in the Upper Sco OB association, but rather a small excess of low-mass stars. Our results on the stellar age distribution confirm earlier indications that the star formation process in Upper

  2. The Ara OB1a association. Stellar population and star formation history

    NASA Astrophysics Data System (ADS)

    Baume, G.; Carraro, G.; Comeron, F.; de Elía, G. C.

    2011-07-01

    Context. The Ara OB1a association is a nearby complex in the fourth Galactic quadrant where a number of young/embedded star clusters are projected close to more evolved, intermediate age clusters. It is also rich in interstellar matter, and contains evidence of the interplay between massive stars and their surrounding medium, such as the rim HII region NGC 6188. Aims: We provide robust estimates of the fundamental parameters (age and distance) of the two most prominent stellar clusters, NGC 6167 and NGC 6193, that may be used as a basis for studing the star formation history of the region. Methods: The study is based on a photometric optical survey (UBVIHα) of NGC 6167 and NGC 6193 and their nearby field, complemented with data from 2MASS-VVV, UCAC3, and IRAC-Spitzer in this region. Results: We produce a uniform photometric catalogue and estimate more robustly the fundamental parameters of NGC 6167 and NGC 6193, in addition to the IRAS 16375-4854 source. As a consequence, all of them are located at approximately the same distance from the Sun in the Sagittarius-Carina Galactic arm. However, the ages we estimate differ widely: NGC 6167 is found to be an intermediate-age cluster (20-30 Myr), NGC 6193 a very young one (1-5 Myr) with PMS, Hα emitters and class II objects, and the IRAS 16375-4854 source is the youngest of the three containing several YSOs. Conclusions: These results support a picture in which Ara OB1a is a region where star formation has proceeded for several tens of Myr until the present. The difference in the ages of the different stellar groups can be interpreted as a consequence of a triggered star formation process. In the specific case of NGC 6193, we find evidence of possible non-coeval star formation. Two catalogs are only available at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/531/A73

  3. Photoluminescence properties of Pr3+ doped Bi2ZnOB2O6 microcrystals and PMMA-based composites

    NASA Astrophysics Data System (ADS)

    Jaroszewski, K.; Chrunik, M.; Głuchowski, P.; Coy, E.; Maciejewska, B.; Jastrzab, R.; Majchrowski, A.; Kasprowicz, D.

    2016-12-01

    Photoluminescence properties of red-emitting Pr3+-doped Bi2ZnOB2O6 microcrystalline powder and PMMA-based composite materials were reported. Bi2ZnOB2O6:Pr3+ powders were synthesized by means of the modified Pechini method. The morphology and crystallographic structure of Bi2ZnOB2O6:Pr3+ microcrystals were investigated by XRD and HRTEM. The PMMA-based composite materials were prepared by embedding of Bi2ZnOB2O6:Pr3+ powder in the PMMA matrix. The vibrational properties of the powder and composite systems were investigated by μ-Raman spectroscopy. Emission spectra of the samples were measured under blue (451.6 nm) and UV (320 nm) excitation. Both, the powder and composite samples show enhancement of red emission (1D2 → 3H4) and quenching of greenish-blue luminescence from 3P0 level of Pr3+ ions after excitation in UV and VIS caused by the 3P0 ∼ 1D2 non-radiative relaxation of Pr3+ ion by low-lying charge transfer state and non-radiative de-excitation through Pr3+-Bi3+ energy transfer. The response of the powder and composite samples to pulsed excitation at 451.6 nm was measured by monitoring emission from the 1D2 level (595 nm/3P0 → 3H6 transition). The determined lifetimes τ1 and τ2 of the red emission at 595 nm (1D2 → 3H4 transition) of the composite are significantly longer in comparison to powder samples. Moreover, because of the good nonlinear optical properties of the Bi2ZnOB2O6 crystals and effective luminescence of the Pr3+-doped Bi2ZnOB2O6 powders and composites, they can be very useful as bi-functional materials in the new generation of optoelectronic devices.

  4. A real-time method to reduce ballistocardiogram artifacts from EEG during fMRI based on optimal basis sets (OBS).

    PubMed

    Wu, Xia; Wu, Tong; Zhan, Zhichao; Yao, Li; Wen, Xiaotong

    2016-04-01

    The simultaneous acquisition of electroencephalogram (EEG) and functional magnetic resonance imaging (fMRI) provides both high temporal and spatial resolution when measuring brain activity. A real-time analysis during a simultaneous EEG-fMRI acquisition is essential when studying neurofeedback and conducting effective brain activity monitoring. However, the ballistocardiogram (BCG) artifacts which are induced by heartbeat-related electrode movements in an MRI scanner severely contaminate the EEG signals and hinder a reliable real-time analysis. The optimal basis sets (OBS) method is an effective candidate for removing BCG artifacts in a traditional offline EEG-fMRI analysis, but has yet to be applied to a real-time EEG-fMRI analysis. Here, a novel real-time technique based on OBS method (rtOBS) is proposed to remove BCG artifacts on a moment-to-moment basis. Real-time electrocardiogram R-peak detection procedure and sliding window OBS method were adopted. A series of simulated data was constructed to verify the feasibility of the rtOBS technique. Furthermore, this method was applied to real EEG-fMRI data to remove BCG artifacts. The results of both simulated data and real EEG-fMRI data from eight healthy human subjects demonstrate the effectiveness of rtOBS in both the time and frequency domains. A comparison between rtOBS and real-time averaged artifact subtraction (rtAAS) was conducted. The results suggest the efficacy and advantage of rtOBS in the real-time removal of BCG artifacts. In this study, a novel real-time OBS technique was proposed for the real-time removal of BCG artifacts. The proposed method was tested using simulated data and applied to real simultaneous EEG-fMRI data. The results suggest the effectiveness of this method. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. New Low-Mass Wide Companions to Members of the Sco-Cen OB Association

    NASA Astrophysics Data System (ADS)

    Finn, Molly; Mamajek, Eric E.; Luhman, Kevin; Murphy, Simon

    2017-01-01

    We have conducted a survey for wide common proper motion companions to 512 members of the Upper Cen-Lup (UCL) and Lower Cen-Cru (LCC) subgroups of the Sco-Cen OB association using astrometry and photometry from the SPM4 catalog. Companion candidates were selected within 3 arcminutes (23 kau = 0.11 pc at d = 130 pc). We find that 12% of the Sco-Cen members have stellar companions in the separation range 3-20 kau. Optical spectra of 16 candidate companions were taken with the new COSMOS spectrograph on the Blanco 4-m telescope and the Wide Field Spectrograph on the Australian National University 2.3-m telescope. The spectroscopic survey yielded 14 M-type stars with Na I surface gravity index indicative of pre-MS status, of which half were Li-rich (along with two background giant interlopers). Seven stars in the range M1-M4.5 appear to be Li depleted, but otherwise seem to be pre-MS due to their chromospheric activity, low surface gravity via the Na I index, and their co- movement with other young stars in Sco-Cen. We suggest that these stars represent the Li depletion boundary for UCL and LCC. We acknowledge support from NSF award AST-1313029 and the REU Site in Physics and Astrophysics at the University of Rochester supported by NSF award PHY-1156339.

  6. VizieR Online Data Catalog: 2007-2016 Pulkovo Uranian satellites obs. (Ershova+, 2017)

    NASA Astrophysics Data System (ADS)

    Ershova, A. P.; Roshchina, E. A.; Izmailov, I. S.

    2016-11-01

    Observations of the main Uranian satellites were made with the 26-inch refractor (D/F=65cm/1041.3cm) at the Pulkovo Observatory in 2007-2016. The CCD camera FLI Pro Line was used (3056x3056px, each pixel of 12-microns). The field of view -- 12'x12', focal plane scale -- 19.8"/mm, scale on the CCD frames -- 0.24"/px. The UCAC4 catalog was used as a reference. Coordinates' epoch is the J2000.0. Average values of Standart Error of the Mean (SEM) of RA and DEC for each satellite are shown in table below. Satellite RASEM DECSEM ------------------------------------------------ Ariel (U1) 0.06" 0.06" Umbriel (U2) 0.05" 0.07" Titania (U3) 0.02" 0.02" Oberon (U4) 0.02" 0.02" ------------------------------------------------ Coordinates with their errors are shown in the "obsperr.dat" file. The "obs.dat" file was made according to MPC format and contains data in the same order as "obsperr.dat". Both files contain date of observation and satellite index so sit is easy to compare strings. (2 data files).

  7. Multiple kinematical populations in Vela OB2 from Gaia DR1 data

    NASA Astrophysics Data System (ADS)

    Damiani, F.; Prisinzano, L.; Jeffries, R. D.; Sacco, G. G.; Randich, S.; Micela, G.

    2017-06-01

    Context. Recent results using radial-velocity measurements from the Gaia-ESO Survey have led to the discovery of multiple kinematic populations across the Vela OB2 association. We present here a proper-motion study of the same region. Aims: Our aim is to test whether or not the radial-velocity populations have a counterpart in proper-motion space, and if so, how the two sets of kinematical data complement each other. Methods: This work is based on parallaxes and proper motions from the TGAS catalogue, as part of Gaia DR1. Results: Two distinct proper-motion populations are found dispersed across 5 degrees (or 30 pc at their likely distances). Their detailed correspondence to the radial-velocity populations could not be tested because of the paucity of common objects. However, compelling indications are found that one of the new proper-motion populations consists mostly of members of the young cluster NGC 2547, and the other is related to the γ2 Vel cluster. Constraints on the age of the two populations, both of which appear to be only 10-35 Myr old, and their possible mutual interactions within the last 1.5 Myr are discussed.

  8. THE GALACTIC SPATIAL DISTRIBUTION OF OB ASSOCIATIONS AND THEIR SURROUNDING SUPERNOVA-GENERATED SUPERBUBBLES

    SciTech Connect

    Higdon, J. C.; Lingenfelter, R. E. E-mail: rlingenfelter@ucsd.edu

    2013-10-01

    The Galactic spatial distribution of OB associations and their surrounding superbubbles (SBs) reflect the distribution of a wide range of important processes in our Galaxy. In particular, it can provide a three-dimensional measure not only of the major source distribution of Galactic cosmic rays, but also the Galactic star formation distribution, the Lyman continuum ionizing radiation distribution, the core-collapse supernova distribution, the neutron star and stellar black hole production distribution, and the principal source distribution of freshly synthesized elements. Thus, we construct a three-dimensional spatial model of the massive-star distribution based primarily on the emission of the H II envelopes that surround the giant SBs and are maintained by the ionizing radiation of the embedded O stars. The Galactic longitudinal distribution of the 205 μm N II radiation, emitted by these H II envelopes, is used to infer the spatial distribution of SBs. We find that the Galactic SB distribution is dominated by the contribution of massive-star clusters residing in the spiral arms.

  9. A novel scheme of signaling protocol based on mobile agent and OBS

    NASA Astrophysics Data System (ADS)

    Mao, Minjing; Liu, Deming; Li, Wei

    2005-11-01

    A novel scheme of signaling protocol based on mobile agent and OBS which runs over a core DWDM network is promoted. Design of optical wavelength channels in the scheme is to choose 1310nm band to be control optical wavelength channel to transmit BHPs and 1550nm band to be burst packets optical wavelength channels to transmit BPs. Referring to RSVP-TE in DCM ASON, significations and formats of signaling messages in the protocol are defined. The key design of scheme is to utilize the structure of active packets in the application of mobile agent MANet to encapsulate signaling messages and traffic together into one BHP to shorten the set-up time of an optical path. Such structure is also used to process distributed disposals to alleviate the workload of one node in the network to promote cooperating calculation ability of each node in the network. The communication between a source node and a destination node through one or multiple intermediate nodes is presented as an example to describe the processing procedures of the protocol. The scheme is implemented in a wavelength router, which is briefly introduced.

  10. BOREAS TE-1 CO2 and CH4 Flux Data Over the SSA-OBS Site

    NASA Technical Reports Server (NTRS)

    Anderson, Darwin; Papagno, Andrea; Hall, Forrest G. (Editor); Newcomer, Jeffrey A. (Editor)

    2000-01-01

    The BOREAS TE-1 team collected various data to characterize the soil-plant systems in the BOREAS SSA. Particular emphasis was placed on nutrient biochemistry, the stores and transfers of organic carbon, and how the characteristics were related to measured methane fluxes. The overall transect in the Prince Albert National Park (Saskatchewan, Canada) included the major plant communities and related soils that occurred in that section of the boreal forest. Soil physical, chemical, and biological measurements along the transect were used to characterize the static environment, which allowed them to be related to methane fluxes. Chamber techniques were used to provide a measure of methane production/uptake. Chamber measurements coupled with flask sampling were used to determine the seasonality of methane fluxes. This particular data set contains carbon dioxide and methane flux values from the SSA-OBS site. The data were collected from 09-Jun to 04-Sep-1994. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  11. Availability and characteristics of abortion training in US ob-gyn residency programs: a national survey.

    PubMed

    Turk, Jema K; Preskill, Felisa; Landy, Uta; Rocca, Corinne H; Steinauer, Jody E

    2014-04-01

    To assess the availability and characteristics of abortion training in US ob-gyn residency programs. We surveyed fourth-year residents at US residency programs by email regarding availability and type of abortion training, procedural experience and self-assessed competence in abortion skills. We conducted multivariable, ordinal logistic regression with general estimating equations to determine individual-level and resident-reported, program-level correlates of quantity of uterine evacuation procedures done during residency. Three hundred sixty-two residents provided data, representing 161 of the 240 residency programs contacted. Access to training in elective abortion was available to most respondents: 54% reported routine training--where abortion training was routinely scheduled; 30% reported opt-in training--where training was available but not routinely integrated; and 16% reported that elective abortion training was not available. Residents in programs with routine elective abortion training and those who intended to do abortions before residency did a greater number of first-trimester manual uterine aspiration and second-trimester dilation and evacuation procedures than those without routine training. Similarly, routine, integrated training, even for indications other than elective abortion, correlated with more clinical experience (all p<.01, odds ratio and confidence interval shown below). There is a strong independent relationship between routine training and greater clinical experience with uterine aspiration procedures. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. New Version of SeismicHandler (SHX) based on ObsPy

    NASA Astrophysics Data System (ADS)

    Stammler, Klaus; Walther, Marcus

    2016-04-01

    The command line version of SeismicHandler (SH), a scientific analysis tool for seismic waveform data developed around 1990, has been redesigned in the recent years, based on a project funded by the Deutsche Forschungsgemeinschaft (DFG). The aim was to address new data access techniques, simplified metadata handling and a modularized software design. As a result the program was rewritten in Python in its main parts, taking advantage of simplicity of this script language and its variety of well developed software libraries, including ObsPy. SHX provides an easy access to waveforms and metadata via arclink and FDSN webservice protocols, also access to event catalogs is implemented. With single commands whole networks or stations within a certain area may be read in, the metadata are retrieved from the servers and stored in a local database. For data processing the large set of SH commands is available, as well as the SH scripting language. Via this SH language scripts or additional Python modules the command set of SHX is easily extendable. The program is open source, tested on Linux operating systems, documentation and download is found at URL "https://www.seismic-handler.org/".

  13. Central stars of planetary nebulae. II. New OB-type and emission-line stars

    NASA Astrophysics Data System (ADS)

    Weidmann, W. A.; Gamen, R.

    2011-07-01

    Context. There are more than 3000 confirmed and probably known Galactic planetary nebulae (PNe), but central star spectroscopic information is available for only 13% of them. Aims: We have undertaken a spectroscopic survey of the central stars in PNe to identify their spectral types. Methods: We performed spectroscopic observations at low resolution with the 2-m telescope at CASLEO, Argentina. Results: We present the spectra of 46 central stars of PNe, most of them are OB-type and emission-line stars. Based on data collected at the Complejo Astronómico El Leoncito (CASLEO), which is operated under agreement between the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina y Universidades Nacionales de La Plata, Córdoba y San Juan, Argentina.The reduced spectra (FITS files) are available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/531/A172

  14. Isolation and Purification of Cu-free Methanobactin from Methylosinus trichosporium OB3b

    SciTech Connect

    M Pesch; I Christl; K Barmettler; S Kraemer; R Kretzschmar

    2011-12-31

    The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS. The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements, and UV-vis spectra indicated that methanobactin was only present in one of the HPLC fractions. On a mass basis, methanobactin carbon contributed only 32% to the total organic carbon isolated with resin extraction. Our spectroscopic results implied that besides methanobactin, the organic compounds in the resin extract comprised breakdown products of methanobactin as well as polysaccharide-like substances. Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin. The proposed HPLC purification procedure is suitable for semi-preparative work and provides copper-free methanobactin.

  15. The OB hospitalist and the risk manager: ready for prime time.

    PubMed

    Veltman, Larry

    2011-01-01

    In 1996, Wachter and Goldman described a new model of care in which hospital-based physicians provided patients' inpatient care in lieu of the patient's primary physician.(1) They termed these physicians hospitalists. The hospitalist movement had taken hold, and by 1999, 65% of internists had hospitalists in their community and 28% reported using them for inpatient care.(2) In 2003, Louis Weinstein, in an article entitled "The Laborist: A New Focus of Practice for the Obstetrician"(3) advocated for the adoption of the hospitalist model to obstetrical care. In a 2010 study, of 28,545 members of the American College of Obstetricians and Gynecologists (ACOG) contacted in a national survey, 7,044 clinicians responded, which yielded a response rate of 25%. Of the respondents, 1,020 clinicians (15% of respondents, 3.6% of the entire sample) described themselves as obstetrics/gynecology hospitalists or laborists.(4) According to the web site www.obgynhospitalist.com, there are at least 115 hospitals in the country that utilize a laborist or OB hospitalist model of care.(5). © 2011 American Society for Healthcare Risk Management of the American Hospital Association.

  16. H-alpha Monitoring of the Star Field around Cygnus OB2